LOCUS       DAOPXU010000001        73371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_226_length_73371_cov_12.524715, whole genome shotgun sequence.
ACCESSION   DAOPXU010000001 DAOPXU010000000
VERSION     DAOPXU010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 73371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 73371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73371
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_226_length_73371_cov_12.524715"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..234
                     /locus_tag="PKK03_00005"
     CDS             37..234
                     /locus_tag="PKK03_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82797.1"
                     /translation="MDKILIYNDRHAFREWLGKCGAESDGIWILFSKNEKIVTLSAAE
                     ALEKALCHGWTDGQIQSPDDI"
     gene            366..1472
                     /locus_tag="PKK03_00010"
     CDS             366..1472
                     /locus_tag="PKK03_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82798.1"
                     /translation="MKIYLRLIVASLILAFSILADAQSDFRRGYIITNDDDTLVGFID
                     FRGYTSNSQKCGFKPAENAPPSVFKPGDIKAYRIMDDKYYVSRPVETEEGQKEMFLEY
                     LIKGEASIYYYRDNKGEHYLIDKRGGPLTELPYHKEEAIIDEVPRFLESTKHIGLLKA
                     YMSDCPALFNRIESLKKPDHDNLIALAKDYHQLICNDNSCIVYSKRKPALGVAAEPAV
                     IFNWFRYGTGIYTGTGISLHFWLPRSSERLYFRTGYMYTRGVLYQRRNDEMEWIGIER
                     SIHRIPVQFEYLVPGRVVQPRFNYGINLIGTDDYDKAFDQTAGAGLLVKVYNQLYISG
                     GVEADWTPILLSLMMDFDIELVSLSLNFGLYYRF"
     gene            1508..1960
                     /locus_tag="PKK03_00015"
     CDS             1508..1960
                     /locus_tag="PKK03_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82799.1"
                     /translation="MSNRFIIHRGELVFEEDCLLIKDGQPAWFRIASIVSLIAIMLYG
                     VLVIIKYFRGDDSSDLWIGVTIISLGIPAMIVQSKISYDSKIEYKHIRSIKIRRNLTD
                     QLLADILLNNRKKRRIVLDSEDLGRFGRDHLDKLIEEFNDKQVQTIPA"
     gene            2044..2583
                     /locus_tag="PKK03_00020"
     CDS             2044..2583
                     /locus_tag="PKK03_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018107424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNX82800.1"
                     /translation="MKGINPLLALFALIIFTAFSSCASIPKGATAVSPFDKERYLGKW
                     YEIARLDFKFERNLNNTTAEYSLNGNGTIKVDNQGYNTVREERTQAIGKAKFAGDDKV
                     AMLKVSFFGPFYSGYNVIALDDEYRYALVAGESLKYMWILSRETTIPDEIKTKYLKLA
                     EEIGYNTSELIWVEHNNSR"
     gene            2594..3145
                     /locus_tag="PKK03_00025"
     CDS             2594..3145
                     /locus_tag="PKK03_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82801.1"
                     /translation="MEFDRQRVTETARREIMAGKSKEEVFRDIYASFNDPRLHEPIAR
                     IVQYIPEPARIRKYGIINTIFMFLLAAICIALIIRSQYTGMVIPAILLYLVSTRQTRY
                     YQWITFVGGLLLIISLTMAITGWPEVKENPLLTLGLATLTGVLFILFGILMPRLLTPG
                     YRAVEETTTDSDGRTVTHKKLIF"
     gene            3197..3682
                     /locus_tag="PKK03_00030"
     CDS             3197..3682
                     /locus_tag="PKK03_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNX82802.1"
                     /translation="MKKLEDILLLGNPLLYEVSEPVLESERDLLPGWKADLHGVMEEV
                     RAKYHFGRGIAAPQLGIMKRVIYINTGKPLLIINPELHDLGDEMMELWDDCMCLPNLL
                     VKVRRNRSLTLRYLDERWENSEWHVTDDLAELVQHEYDHLNGVLCTMRAIDGKSFRWK
                     T"
     gene            3818..4531
                     /locus_tag="PKK03_00035"
     CDS             3818..4531
                     /locus_tag="PKK03_00035"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNX82803.1"
                     /translation="MTATTTTTAASAAATATATATVIGATGLIGGHLAELLTKDDYFK
                     KVRLITRRMHENTPPGAEVVIIDFNDPVAFRSAVAGSDAVFCAIGTTRKKVKGDMDAY
                     RKVDHDIPVNTARHCQEAGCRHFALVSSVGASAKSGNFYLKFKGEVEEAVALMSIPSV
                     SVFRPSMLMGKRNEFRLAEEITKIFAPPLSFLFPAKYRPIKGHDVAQAMIAAAKRAEP
                     GFRVYHFNEMKALIKDREV"
     gene            4565..5038
                     /locus_tag="PKK03_00040"
     CDS             4565..5038
                     /locus_tag="PKK03_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX82804.1"
                     /translation="MEVNIRRAVEEDFPAILKLIKELAEYERSPEAVTNTVEQMRREQ
                     NHFRCFVADNAEDGIMGMALYFFAYFTWVGKSIYLEDIIVSKLFRNRKIGAALMQRVM
                     QEARDENCKRVRWQVLDWNEPAIGFYRKCGAEISGDWLNCTFDEKGISLFNEMMS"
     gene            5038..5727
                     /locus_tag="PKK03_00045"
     CDS             5038..5727
                     /locus_tag="PKK03_00045"
                     /inference="COORDINATES: protein motif:HMM:NF027752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5034 domain-containing protein"
                     /protein_id="HNX82805.1"
                     /translation="MRIDIRHQAGGFAGALRIYSGRTMKVTLLLSVIFILKTITSCID
                     PAEWDFELSFNKITITNLDNSGFYPTETNSDIMYAPAVAFTVTLSDETILASGVNRRS
                     NNSFTGFTPAYAMSPEPRYYPLNRITGISIVTLEAMSPEIPAGTDVTGLFAACVPRFN
                     APGFLYIDSSELLSAIDRDFYPDEPSVTFQLFCREEIAGDKAQFEINITLSDESTLSA
                     TTNLITLVRNE"
     gene            5738..6601
                     /locus_tag="PKK03_00050"
     CDS             5738..6601
                     /locus_tag="PKK03_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82806.1"
                     /translation="MRRYAKIMMFLLPAVAVSSLLPAQGRFIEKKEASLWFGVRDITR
                     DSLANGANLDFLNYLHSSDEMAEYQYLGLSAHLWFRGNWEADFKVAMYDDFAPTSLNI
                     KAIWMPYKYLGLTAGFYSYPQLMNEFNMFHRLSDEGWYGDTDSNFRQRRVHETGIMAG
                     VAVPVSYRFIHLNLCLNGGISTLSKFREKVTQKMINGNMRREIRYSTKNSPALFFFPE
                     AELGLDCFRIGSSTLGVRGHASWFTVKRSVDYKMTIWEWTYDNPMQEDVANPAHSFSK
                     FEFDAGLYLTW"
     gene            6699..7931
                     /locus_tag="PKK03_00055"
     CDS             6699..7931
                     /locus_tag="PKK03_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HNX82807.1"
                     /translation="MSELINNSSERKKRLKTLILRLHQGEAEEKVREDLLSTLSQIPY
                     GEVVEVEQELISEGLPEEEVLRLCDAHSAVLHGNVDLSAAKKIPAGHPVDVLINENKE
                     LRKVVAEIENLISAISYDSGPVKDTEKFRLTGLFNSLWDVDKHYQRKEYLLFPYLERQ
                     GITGPPKVMWGKHDEIRELIKGGIELLRSGEITREELVASAEIVLLPAAQGVKDMTTK
                     EEEILFPMALDNLNEADWYAIQKQSLEIGYCLYDPRVTWKPEWAQEDILHEMNKNADT
                     VQLPSGSFTIEELLAILNRLPIDMTFVDRNDKVKYFSQSEERIFQRNRAILNRDVRHC
                     HPPASAHIVDKIIEDFRSGKASRAPFWINIGGKMIYIEYFALRNDKGEYLGTLEVSHN
                     VTPYRELEGEQRILSYSK"
     gene            7934..8455
                     /locus_tag="PKK03_00060"
     CDS             7934..8455
                     /locus_tag="PKK03_00060"
                     /inference="COORDINATES: protein motif:HMM:NF020553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HNX82808.1"
                     /translation="MDQNKIIISPKTKVLALIEAYPQLENTLISYVPVFEKLKNPVLR
                     RTVAKIATLQQAAVIGNVKVEELINVLRKEVGQDLIAGAAEQELNTVMPSWFDPALVK
                     QETDIRPMLAAGEQPVNQVMADLNTLEAGHIYKVIAPFVPAPLIEKAASLNIDHWLKQ
                     EADELFNVYFRRS"
     gene            8471..9352
                     /locus_tag="PKK03_00065"
     CDS             8471..9352
                     /locus_tag="PKK03_00065"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNX82809.1"
                     /translation="MEIKSDHTDKVYSYLALRKAVGWIGILLPIVLVLGHLIIFDGES
                     ALTNMSVYYHTGMRDVFVGAICAIALFLFFYRGYDRWDNRAADLAGLCALGVAFFPTV
                     EEGSWDWVAWIHFTSAACFLVILALISIFLFTRGEKHPTEMKKKRNLIYRACGIVMLA
                     ALASIEVFFLFFDGVNSDSRFVLIAETITLMAFGISWLTKGGTLYPDKPIKKNDMEND
                     EKLIRVFVAPEPTVMLLLEMLEETGIKGLIKNDSGLGYLGAVPQVMDLYILEEDLEKA
                     TPLINEFKEKHNSEKDS"
     gene            9366..11030
                     /locus_tag="PKK03_00070"
     CDS             9366..11030
                     /locus_tag="PKK03_00070"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNX82810.1"
                     /translation="MKKTFLPAAIILVLIAVSCNVSTQTSDMIITNARIWTGNAVQPY
                     AEAMAVSGDTIIAIGSNREVMKYKSGSGEVIDLGGRFVAPGFIDSHLHLLQGGSNLAS
                     VQLRDASTPEEFISRIKEYAATLKPGTWILGGDWDGIGWESLPQKDWIDSVTPDNPVF
                     VSRLDGHMALANSLAMKLAGVDRKVKDVSGGTITRDGSGEPTGIFKDNAMDLISVRIP
                     EQSEEEVDRAIVAAMHYLASNGVTSAHAVDAAAYSDALERVRARGEQITRLYYLQTIS
                     RWKDMKEQVERDGRGDRWISTGGVKGFVDGSLGSHTAAFLEPYADLQTDTGLFVNSEE
                     DLYNWILESDKAGLQVVIHAIGDEAINFLLNAYEKVAAVNGERDRRFRIEHSQHIAPA
                     DIERYGKLKVIASMQPYHAIDDGRWAEEYIGPERVKTTYAFRSLLDSGATLAFGSDWF
                     VAPATPLEGIYAATTRRTLDGKNPGGWVPEQKITVEEALKAYTINAAYASFEEDFKGT
                     LEPGKLADFVVLDRDITAIDPVEIWNVKTQQTWVGGKKVFDRTEAR"
     gene            11042..12208
                     /locus_tag="PKK03_00075"
     CDS             11042..12208
                     /locus_tag="PKK03_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNX82811.1"
                     /translation="MIITRQLIRTAAVMMLLLPVLNCQNTDAQMKRARDYGIEIGILR
                     TGSYNAITDVPGVKVGQVTLKEGDSVRTGVTAILPHEGNIFQQKVPAGIFVGNGFGKL
                     AGYTQVEELGNLETPVVLTNTLSVPTAVAAVIEHTLSQPGNEGVTSVNAVVGETNDAR
                     INDIRGRHITTEHVLEAIRSAAGGPVAEGNVGAGTGTVCFGFKGGIGTSSRVLPVTRG
                     GYTVGVLVQTNYGGILQVNGVPVGQELGRYSFRTTVNEYKEDGSCMIVVMTDAPLTSR
                     NLKRLGSRAFAGMMRTGASGSNGSGDYIIAVSTAEELRIPYSSESMYEQWHEIRNDEM
                     DLLFQAAAEATEEAIINSLFAAETTKARNGMTVEALPVEKTLEIMRKYNRLKER"
     gene            12338..13393
                     /locus_tag="PKK03_00080"
     CDS             12338..13393
                     /locus_tag="PKK03_00080"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNX82812.1"
                     /translation="MFTGLDSLINAEFQAQEPGGALLVMKGDSAVYLSSFGLADMVTG
                     EKITENTLFNLGSISKTFVANGILILQEEGKLSVEDPLSKYFPEFKRRELAKKVTVMH
                     LLTHTSGLPDNRPVSENPEFFLTAKDYENWAPVTQAENLVFEPGEKYEYSNPAFNGLA
                     LIIEKVSGQKWQDFIAERIFKPAGMATSTITDGPHPETGVSHAYEMTGGKWAESDYGE
                     FPTFPAAGNGGVWSSVRELALYEKAIRENRFLGAELTGKSRTICRPDTWAGNESPFIG
                     YSWFIGEEQILGQANVFGVKFVYHTGDQGGFRAFHFSVPEKDLLYVGLFNRPPEDMDR
                     IIIKGLSILKENNWLDN"
     gene            13478..14434
                     /locus_tag="PKK03_00085"
     CDS             13478..14434
                     /locus_tag="PKK03_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HNX82813.1"
                     /translation="MTIQILLLLAGLALLIISAEGLVGGATSLARRFNISDLTIGLTV
                     VAFGTSAPELVVNLIASSSDHQDIVFGNILGSNNFNLYIILGIAGLILPLKVHISTVW
                     REIPMSLVALVIFFFMVNIPSAGGGRMLGRTDGLILLALFVSFQYYIFHQIKNLEREP
                     SEHKNPGPLYRTIGFIVLGLAGLVIGGRLVVTNAVFIAKAMGVSEKVIGLTIIAAGTS
                     LPELATSLVAAFRKNPDIAVGNVVGSNIFNILLIGGLVSVVRPVAYNPVFNTDFYILA
                     AGTVLLFITMFTSQRHKLDRWEAGLLLAVYVGYTTWLIMKEV"
     gene            complement(14455..16323)
                     /locus_tag="PKK03_00090"
     CDS             complement(14455..16323)
                     /locus_tag="PKK03_00090"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNX82814.1"
                     /translation="MNTEPTLKLNPFFRTAATVALIIAFVAPVCSQPSGGPYGPVRQV
                     WPVPEKAGNTFYVAPDGNPGASGISAAAPSTIEAAIAKATTGDVIIMRGGTYRTGDLV
                     LNQGITIQPYGDELPVLKGTGVATQWNDLGNGLWVTKWDRLFPSAPADWWQRLRSGKE
                     TPLHRFNDDMVFIDGRFLQSAGYEGEVNENTFFIDYSTGLVYIGVDPKDKLVEITAYN
                     IGIHRITGEYNGRPSDGRGPVIRGITFTQYAYRAIEIDGKDPEGISPEAEHGKDVIGT
                     TLEHCTISFCSRVAAYLRGDRLTIRNCRVSDTSTEGIYVLSSSDVLLEKNIFTRNNIE
                     RISGYYPAAVKIFNQCYRVTCNDNLVTDLPFSNGIWYDVGNVDAVFTNNWVENVGLTG
                     RAVSYLQTWPSESGFFFEISKGAVCAGNVFVNCELGIHILNSCDAVMYNNTLVNSTAC
                     ISRSERSAVGDHFGWHPATGPGVDERDGHVFVNNLMVADATLNRPHLFVWQRAMLCGR
                     LRDPQFSTIDNNVYVRTGERKEMPLIVWSPVQNEQCIAEMNSPEEINKILPGFETKSI
                     LIKDYSTFMSPELKNFRPLPSFYLNARPAPFPQEVMSVFRLKKKSTPWVGAYPPQL"
     gene            16393..17232
                     /locus_tag="PKK03_00095"
     CDS             16393..17232
                     /locus_tag="PKK03_00095"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNX82815.1"
                     /translation="MKTKSIIPGVIILLASISAGGQDLQVKQSLAEIEWNIKNITAGV
                     ILKSARARLFDSDQAIYVVETDTTAGRFEIGVAAGDKAIVTSAYAAGEGVLAAINGTF
                     FNMEKGYNVHYVKVDDTVVSVTDENEYGIRATGVFTATGEAVDIASWGPEREDAGYLN
                     AEDAIVTGPLLTDNGKDIAIDSTNFNKNRHPRSLLGITGEGHVLFIAVDGRQSGYADG
                     MSLFELRQLARSLGCTEILNLDGGGSTTLYVKGEGVSGVVNKPSGRAERPVPSIVFLK
                     KIE"
     gene            17251..17832
                     /locus_tag="PKK03_00100"
     CDS             17251..17832
                     /locus_tag="PKK03_00100"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNX82816.1"
                     /translation="MSYTLKPGEKAIDFRLPATDGREYSLADFDDHPYLVVFFTCNHC
                     PYVIGSDEVTRATAEKFRDRGVAFVGINSNSEHTYEEDSFPNMVKRMKEHNFPWKYLY
                     DRDQQAAKDYGALKTPHFFVFNRDRVLAYTGRGVDSPRDTTRMTVNDLDRALEELTTG
                     RPISVAVTNPIGCNIKWEGREAKWMPPEACDLV"
     gene            17867..18754
                     /locus_tag="PKK03_00105"
     CDS             17867..18754
                     /locus_tag="PKK03_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX82817.1"
                     /translation="MSTPAIQISDLTKRYGKDRGIENINLQVEEGEIFGFIGPNGAGK
                     STTIRVLLNLLFPTGGSARIMGLDILRDSKKIRAQTGYIPSEVNPWPDMTAREFLRYS
                     ASFYGIAHDAKKIPELMEMFDVEPGRKISDLSSGNRKKLSIIQSFLHTPALLIVDEPT
                     TGLDPLMQSRFFDILRSENRRGMTVFFSSHSLSDVQMLCGRVAIIREGRIVNVEAIET
                     LRKKQLKKIRIEFVDRIPEILTGMPGMKNGVNVSGKAMEFIWSGDMNGLIAALSGKEI
                     ADMTVEEPSLEEIFMHYYN"
     gene            18769..19578
                     /locus_tag="PKK03_00110"
     CDS             18769..19578
                     /locus_tag="PKK03_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNX82818.1"
                     /translation="MNRNLFLREFRSNATGLLIWTAIIAFLTTATMSLYSIFLENNTK
                     ILAMISILPEGALQFKGVSNVNDLFSVLGFYSANNVIYMLVLGSIYSIVLSSNILLKE
                     EYGRTAEFLLSWPVTRGQVFFSKLAVVVINILAINLIITVAGYISLETCKKEPYSVDA
                     FFIMSVYTLLLHFFFATLGLFMSVLVKKPKPITTLGVAWVMVLYFIFTISNITPDLSW
                     AGYVSPYKFIDLDVVNPAYALDLVNIMYFTLFSFLLIFFSLRIYLRKDIYL"
     gene            20268..21185
                     /locus_tag="PKK03_00115"
     CDS             20268..21185
                     /locus_tag="PKK03_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNX82819.1"
                     /translation="MTRPAWPGLVPYRDFLGSNLEILDIETGLRRVVFQAPNSIQAPN
                     WTPDGRYLIYNSEGLLYRFDLESGRPEVINSGFATSNNNDHVLSFDGKMLGISHHAAE
                     EDNKSIIYIMPAEGGTPERITAKGPSYLHGWSPDGRWLTYTAERNGDYDIYKISVKKR
                     KEIRLTDTSGLDDGSEYSPDGKYIYFNSARTGTMKLWRMKPDGSKQEQLTFDEYNDWF
                     PHISPDGKQIVFLSFMPEVKADDHPFYKHVYIRKILIPGADAGRQKFAPLTPEVIVYL
                     YGGQGTINVPSWSPDGKKIAFVSNSGPTE"
     gene            21190..22944
                     /locus_tag="PKK03_00120"
     CDS             21190..22944
                     /locus_tag="PKK03_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HNX82820.1"
                     /translation="MIRIRKITNPFHEANTRKIEAVKEILRKQFPAISEKKIGELETQ
                     FVNPLSKKYQTAVFVAEDIHEAVRAFAVLLFMPDLQFCYLDYIAASPDSSSSGLGSAL
                     YERIREEAISLDAIGLFFECLPDDPVLCHDPETLEQNRKRLAFYERFGARPVAGTLYE
                     TPVKPEDNCPPYLVFDGLGHRTAVSRDELRGIVRAILERKYGDYSGEEYNNMVINSIT
                     DDPVAIRPPRYTRRKILPEPTAAIPEKKKIWLAVNDRHTIHHVRERGYVESPVRVKSI
                     LKELDKSGLFRAGRVREYPDRHILDVHDRNYFNYFRKVSRNIPRGQSVYPYVFPIRYA
                     SRAPKDLSVRAGYYCFDTFTPLNQDAFLAARWGVNCVMSAADELLAGTRYAYVLTRPP
                     GHHTERSVFGGFCYFNNCAIAAHYLSRSGRVAILDIDYHHGNGQQQIFYGRSDVLTLS
                     IHGHPSFAYPYFSGFEEEKGEGKGLGFNHNFPLPEKVTHEQYVKVLSRALNLIKKFKT
                     DYLIVALGLDPAKGDPTGTWSFTHNNFTANGEMIGSMKLPTLVVQEGGYRNQSLGINA
                     RAFFTGLHRAHNGKNPMK"
     gene            22941..23801
                     /locus_tag="PKK03_00125"
     CDS             22941..23801
                     /locus_tag="PKK03_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82821.1"
                     /translation="MKKSIFTMTGIKRYLLVAGILLVAVILLANKLVIWGAAFIAAGL
                     IILGIQQLIQLNVKVSKFEDNISGLQEKNQDLAKMNEKLIEENAFLKERHFQITQIKS
                     ILEMNLFEIDTRFKRTVNRTEMLNDREIRHIGSLGVSLKAKYGIDLKELRFKYMPESD
                     ELIVANINPRFLSFGNRKLEWEFFETLEYRRQLPLTTKRWMTSDDLAEKADRLKEEIR
                     LETEHSLESGPEEFSWIMEPIRENVENTIRIMFRGICSNIRIAELADNSFVPLEGIRL
                     ERLEEQKKLK"
     gene            23809..25758
                     /locus_tag="PKK03_00130"
     CDS             23809..25758
                     /locus_tag="PKK03_00130"
                     /inference="COORDINATES: protein motif:HMM:NF025861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HNX82822.1"
                     /translation="MKRIWIAALVVIALASGCATSGTVDMASPDGRIVVAFNLLEGGQ
                     PVYSVTYRDTAVITEGKLGLIRSDAGFSSGLRLNSVTGPEEVKENYTLLHGKKRDASY
                     HAFRYTVHLSGASGEAMDIIFQVSDEGLAFCYKFPGVSEEIKYITAEKSSFGFAEGAL
                     AWIQPRAKAKTGWNQSNPSYEENYLMEVPLANVAADTNGWVFPALYRSGGCWVNLTES
                     WPDRNYCGSHLAKGEGANEMMIAFPEPTEGYTGGSVFPESKLPWQTPWRIVTMGSTPG
                     AIAESTHGTDLALPPAEGEFSYAKPGRAAWSWALMKDGSVNYEEQKRFIAYAAEMGWE
                     YCLIDVNWDQTIGWDRIEELAKLAQAKKVGLILWYNSAGDWNTVTYHPKDRLLTHESR
                     EEEFSRLESLGIKGIKVDFFGGDGQSMMDYYQDILADAYRHKLVVNCHGSTLPRGLHR
                     TWPNLVSMEAIRGFEYATFDQGTADLVASKATTLAFTRNAFDPMDFTPVCFTEYDNNK
                     RVTGNGAELALAVLFLSGVQHYAETPQGMATVPDYVREMMKEIPVSWDETMFIDGHPG
                     RYIVLARRSGNVWYYAGVNAQKDTLGLNLKLPFAGGISGTIITEGETLRSFTQDTVVP
                     DGSGMATVQIVPGGGFVIRFEKPAL"
     gene            25770..28133
                     /locus_tag="PKK03_00135"
     CDS             25770..28133
                     /locus_tag="PKK03_00135"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HNX82823.1"
                     /translation="MKKNILYLLLSLLTATGCDMPDKSPGDNLLWYDKPAAEWTEALP
                     VGNGRLGGMLYGDPSVEHLQVNEESLWGGVKVPNNNPGALENLPRIRKLILDGNIPEA
                     YELAEEHLSGIPGKTWSYQTLGDIFFRFADTAATVTDYRRELNLSTGIASVSFKRNGK
                     KVEYEVFVPAHEDLIVITMKSQEEGGLDMQIMMTRPRDAEITVTENRIDMEGQVVDLV
                     DPERGPAGEHMKFYASLIITNCDGILTPEENSLGYSGGKEITLFFNARTDYNIDYLNF
                     DRAIDPRKICTEAISKASWTSMSRIRNGHKTEHSSMFNRVKLSLGGDPALNELPTDER
                     LRRFQAGGDDPGLIATYFQYGRYLLMGSSRYPAQLPANLQGIWNKDINAPWNSDYHTN
                     INLQMNYWPAEVCNLTETAGPLIRFMEKLTGPGSVTARDTYGAKGWTLHHTTDVFGKT
                     AVLDGVYWGMFPLGGAWMTLPLWEHYEFTNDTAYLREHAYPVMKGAADFIGTFLVPDS
                     QGRLVTVPSYSPENSYIDPVSGKEFKLTYAPSMDNQIIREVLGNCRKASAILGVDREW
                     ADSMQFILDRVPPDRVTSNGTIMEWIEEFKEAEPGHRHMSHLFALHPGAQITPETPEL
                     YAAARRTLERRLENGGGHTGWSRAWIINFYARLLDGDEAYKHLRQLLEKSTLPNLFDN
                     HPPFQIDGNFGGTAGIAEMLLQSQNGVVQILPALPKAWTEGEVSGLKARGNFEIGIEW
                     LGGVAEKITVKSLSGAPLNLAYRGNINSMETEKGIVYKFDSNLKWIE"
     gene            28121..28669
                     /locus_tag="PKK03_00140"
     CDS             28121..28669
                     /locus_tag="PKK03_00140"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNX82824.1"
                     /translation="MDRVIFFIALSLPVIFFSKQSLFEPHSHGFTRFFSWECIIILFV
                     FNYTAWFKEPFSARQIVSWLLLIVSLWFLIESVVRIRRARKPGVVRVDEKLFRFEQTT
                     EMVTSGIYRYIRHPMYSSLLFLTWGIWFKQPLWYLLPVAFLASWLLYLTARRDEKECQ
                     AYFGEKYTEYMKETKRLIPFIM"
     gene            complement(28698..31202)
                     /locus_tag="PKK03_00145"
     CDS             complement(28698..31202)
                     /locus_tag="PKK03_00145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNX82825.1"
                     /translation="MKKLTGLLLLMLIAVASTAQNKIVCNDWRISGVDQLFLPFFADQ
                     PSVDGKKFDTAALLENVQADDADLKSWPVVTLPDDSLLTSVNRDNQLVLMAGFISSDR
                     WTKASVNISTNAVFELSLNGKKVKSQGKPSDKPVKIDLTLGTGIHEVGLKLISTEKTL
                     RFAADVSAANDSAVLSWSTNPARTLTINDILEGVSISGARLSPSGKYVLIQTTEILPG
                     SGKPQSSLQVYDMELNKNLFALRNNALRADWMPKSDRFYYQIDREGTSDVIVYDMKTG
                     TESVAASGLKSPGRIVWSPAEDYFVYSRNEEAEKTGDLKRVFDNEDRIPGTRNRSYLY
                     IYNSATKVSQPITAGSSSATLHAISPDGRRILFSVSRQDYSEVPFSKQTLYELEISTM
                     RLDTIWIDKLYGGSCQYSPDGTQLLVSGGPETFGSLGVKVSDGRIPNSYDTQLYLFDL
                     KTRKAEPLTRNFDPAAGTARWAPDGKIYLTATERDYVNLYRLDPKRRNFTKIDVPVEV
                     ITSVDYAEKSNVALLRGTGISTPVKLYAIDLSTGKSSLLSDPGADQLAYVLKGRTEEW
                     NFKSSRGITIYGRVYYPVNYDPAKKYPVIVNFYGGTTPTDRSFGGRYPKEVWAAEGYM
                     VYVLQPSGSTGFGQDFSALHVNGWGREAIDDIIEGTKKFLAAFPSADAANVGCVGASY
                     GGFTTMMLQTRTDIFRTAIAHAGISDITSYWGEGYWGYSYSSGATKGSYPWNRRDIYV
                     DNSPLFNADKFSNSILLLHGTADTNVPVGESLQYYAALKILGKDVEMVLVSGENHHIL
                     DYKKRIEWHNTIMSWFDMKLRNRPQHWNEMYPDKNL"
     gene            complement(31254..32828)
                     /locus_tag="PKK03_00150"
     CDS             complement(31254..32828)
                     /locus_tag="PKK03_00150"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HNX82826.1"
                     /translation="MKAKFEVRFSKIIYVLVSVTVAALSGCSGENSSGSPSDQKVILY
                     AEFMHEVNSFSPVITKEVNFKSDHLFYGEDVSASAVKEDKQLAGFLSAVEKSGRGRIR
                     AIPVLQAKSMSGGPVDSLFYRKIKNLIIDAVTGQEKVDGIYLSLHGAMGVQGMFDPEG
                     DLISSLRDTLGTDVKIAVTFDLHANVTRRRAENADIIVGYHTNPHRDHFKTAFKAGDL
                     LIRTVMGEIEPVMVVNKMKLLKGGGINVDFLPPFNKIFSAMKKMEKQEDVLSVSFFPV
                     HIWIDDPELGYSTIAITNDKADLSRELADEISEMAWAVRDVPQPEGNSPGEAVAIAQQ
                     KKVARALGTLVFCDVSDAVGTGTPGESTWILKALVEKGSELTSYIALRDEAAALEAWN
                     SNIGDEVTLTVGGKIDTVYNQPFTYNGVVTLKQETSFGKTLIVKQNGIHLIISELPMA
                     SRTPADFKDLGLSIWKADIVVVKNLFPFRYNYLLYNRKTVNVISPGLSNTDPFALNYV
                     HIPRPIYPLDRIDTWR"
     gene            32940..33536
                     /locus_tag="PKK03_00155"
     CDS             32940..33536
                     /locus_tag="PKK03_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82827.1"
                     /translation="MKAEKRIVVTILMLMTVVVVVAQDGSRAFGEVSLAANNAGMYVL
                     GGWALANMAAGAYGWAAFEGEKKYFSQMNLFWNIVNLSIAGFALYSNYSTDLLSLGDG
                     EIMTKHLKTERLFLINSGLDVAYMGAGILMRHLGTGSERRGDLLKGYGNAVILQGGFL
                     LVFDIVMYFIFRDIRPAVIELTSALQQGTLHSGIRIAF"
     gene            complement(33645..34535)
                     /locus_tag="PKK03_00160"
     CDS             complement(33645..34535)
                     /locus_tag="PKK03_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82828.1"
                     /translation="MTALLTCMAASAQYTIGHARMELRDESRGNRRVKFEAYYPVTAQ
                     AGSAAPSGMTGCKFPVICFAHGYQHPGDQYGNLVNILVPAGYVMLSLTTFEGPLPSHI
                     GYADEVRFLAASVAGLGNDPASPLYGIADTICCLMGHSMGGGAMFHAAAGNADADAVI
                     ALTPYQIRPSAIEAASLVMVPTLIFSGTSDCITSPEKNHQPMYERSGAENKTYISIIN
                     GSHCGMGDSRKCFTAERLAGCRQGLNTEQQSAILARYMVPWLDCFLKGEKEQGALFNQ
                     TLASDSAVNWLRSRPLPEPE"
     gene            34710..35513
                     /locus_tag="PKK03_00165"
     CDS             34710..35513
                     /locus_tag="PKK03_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82829.1"
                     /translation="MDIKQPDLAAGNIYAMEKTLFDLLVWLWISVAVVIFIVLLFITA
                     PFGRHSKNTWGVTIPDRMGWFLMEIPGPVIYLSLVIAGIGPKTLTVWIITGLYLLHYT
                     NRAIVYPLRIRTRGKQMPLVVAIMAVLFNFVNAGFLGYFAGSLQTHYTDAWLTDPRFL
                     TGLLIFIAGMAINMTSDNRLIHLRKKSGDGYQIPRGGLFERISCPNLYGEIVEWGGYA
                     LLCWSLPALSFFLWTFSNLVPRALSHHRWYRSHFSDYPTGRKAVLPYIL"
     gene            35538..36281
                     /locus_tag="PKK03_00170"
     CDS             35538..36281
                     /locus_tag="PKK03_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82830.1"
                     /translation="MKGSYLNWKGRFLWIAIFAISMGFLEAIVVVYLRKLYYPDGFSF
                     PLRLMSADLVTAEWIREITTLVMLAGIGIIAGRNGLQRLFYALFAFGIWDIVYYAALR
                     IFLGWPETLMTWDLLFLIPVSWLGPVLAPVINSLTMIMMALLFIGRQEKGFYIRPGLS
                     DWVLIVSGAVVILYTYLVDYSRLLFESGALSAKESPEAGERLAVMITTYVPDHYKWTL
                     FIIGEALIIAATINVLIRSRKYSKDETVN"
     gene            36265..38361
                     /locus_tag="PKK03_00175"
     CDS             36265..38361
                     /locus_tag="PKK03_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNX82831.1"
                     /translation="MKQLTNLTALIMLLLILPGCGDKKSKSAEATDPWLWLEEVDAEK
                     SLDWAREQNRLSDEVLTVRPEFTELRERFLGVLNDRDRIIYPGITGEMSYNLWQDEKN
                     ERGLWRRMSKQDYLAGKDVWETVLDLDLLSAEEGKKWVFGGASWLGPENRYCLLWLSD
                     GGKDESYIREFDAVAKKFVGGGFSIPESKGSAAWVDRDHILVATNYGPGTMTSSGYPA
                     VAKLWKRGEDPANAVIMHRTDTANMGIFVNGGEVNGKQYVFLNENVGFYESSAYLLTP
                     EGMKALDIPKDVETRGMFREAMLLYLQSDWTLSGMTYKKGTLVSFDIPSFLSGTAVVK
                     TLYEPGERESLAGVMSTRDFIVIATMENVQGKLWIMRPSGDSWVTEAVPVPEFGTVGL
                     AGSDDRSNDYFFAFSNPVTPSTLYYGDGKLIRPIRKQKDYFNATGVKVDQYEVASKDG
                     TKIPYFIVYPAEMAPDCSNPTLVYAYGGFNSATRPGYSSITGIGWIEKGGVYVIANIR
                     GGGEFGPEWHHSALKEKRQNAYDDLFAVAEDLISRKITSAERMGFYGWSNGGLLAGVA
                     MTQRPDLWNAVVIGAPLLDMKRYNKLLAGASWMDEYGNPDVPADWEFISKYSPYQNLK
                     TDAKYPTPLFVTSTRDDRVHPGHARKMAAKMIEMGHPVLYHETIEGGHGAASTNAQEA
                     EMEALIFTYLNMKLGK"
     gene            38717..39070
                     /locus_tag="PKK03_00180"
     CDS             38717..39070
                     /locus_tag="PKK03_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNX82832.1"
                     /translation="MTRTKHVLNALLCLMIILASCGGGKGTEAKKAPEAVAETATVSL
                     SIEGMTCTGCENTICTSLEKVPGVKSVTASHTDGKAVIEFEPGKADTAMLKEAVNGAG
                     YKALKISAVADGEKE"
     gene            39115..40092
                     /locus_tag="PKK03_00185"
     CDS             39115..40092
                     /locus_tag="PKK03_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNX82833.1"
                     /translation="MSRDKKEEGNRSNDLDRVIENSRRDFLRKLTLAGAVTVTGGAAI
                     YSGHRFEKAKHREGYVKVLTEDNRLVEIPVEQMRDFKPDLRMLQSRGREGIAGKRWVM
                     VIDLSKCRNARKCVEACQSAHHLRPYEYHINTLVMQESVNTPAFFMPKPCQHCDNPPC
                     VSVCPVDATFKRQDGIVLIDNDRCIGCRFCMAACPYSARIFHWQEPSKHEEDGNKEYD
                     VELNVPQKKGTISKCLFSADRLREGMLPYCVSACPNGVFYFGDENEDAVTNGTTKETV
                     RFSELIEKNGGYRLMPELGTQPRVYYLPPKNRIFEFEQGEKREYKQEKA"
     gene            40105..41448
                     /gene="nrfD"
                     /locus_tag="PKK03_00190"
     CDS             40105..41448
                     /gene="nrfD"
                     /locus_tag="PKK03_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNX82834.1"
                     /translation="MDEIRFNTPHEKGKIDSLASDILKSVRINAGFLLWMTFLFTLLA
                     ACLFAYIIQLRKGLGVTGLRDFTSWGMYIANFVFFVATSLCGMLISSVLGLIGYNWVK
                     PISRIAEIIAIAFAAVAGLVIISDMGRPDRLPYVFLFGRVQSPILWDVTVVTTYFVLS
                     MLLWFFPMIPDLAIAKDRLEGRPKFLIRAYELLSFRWMHHEVQYRILGQAVRILLILI
                     IPTAFAIHTVTSWLFAVTPRTGWDSTIFGPYFISGAFVSGAAAVIIAMYFFRVNYKLE
                     KYLNARVFERMGRVMILVAIVYLYFNLNEFLVPAYKLKTGDAIHLRELFTGHYAMLFW
                     GIQLIGLVIPILLLLFRQMRKPLPMMIISLFVLCASWFKRYIIVVPPQAHPNLPVQNV
                     PVEWMVYSPTLIETAVTVASIVLVLIIITILSKLFPVIPIWEMAEEKEDAERIIQ"
     gene            41468..42415
                     /locus_tag="PKK03_00195"
     CDS             41468..42415
                     /locus_tag="PKK03_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNX82835.1"
                     /translation="MKYTTGVIICLMLLPSFARGQDWVVPDDRKGRLSTFPFTDDTRK
                     EGEKLYQVNCHSCHGDPGRNNFIALQPPPGDPATEKIQRNSDGEIFHKVSAGRGQMPS
                     FRSVLTTDEIWKVVSFIRSFNRSYVQKVMQVITSSAYPGAIISMKLAYDEATKQVLVI
                     ASATSETGTVPVTGAGVRLLAARYFGKMAVDEEKTTDVNGVATFSVPEGLRGDTAGNI
                     GFSAMFTDEATFGSVSKDTVLMAGMPVIPVSLTANRAMWNTVRKAPVWIILAYGLGVL
                     AVWGFIVLVMFKLRDIFTVGTALSENISASHGQTEPTKN"
     gene            42421..42807
                     /locus_tag="PKK03_00200"
     CDS             42421..42807
                     /locus_tag="PKK03_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82836.1"
                     /translation="MKNLAPIGRILFALPFAVFGINHLLMMDYYLGMLTSFIPLGGYT
                     IILTGLVMIIVSISIITKKFIRLSTLILAGLLLIFILTIHLPHLFDGTDRVPIMIALL
                     KDISLMGGSLMIAGYCAEEETKKAEK"
     gene            42804..43586
                     /locus_tag="PKK03_00205"
     CDS             42804..43586
                     /locus_tag="PKK03_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNX82837.1"
                     /translation="MKIFKVAALAFFIFLFSPAGAQEIKTAGYKQPEPEVGVIEHLGD
                     TIPLDLWFYNENSEQVTLRSLIDRPTILSFVYFDCPNLCSPLMDGIADVVGKMDMTLG
                     TDYKIITISFNTSDTPEKAKEKKQNFVQRISKENQKDWIYLTGTQENISAITAAAGFK
                     YTPQGLDFAHPSVITVVSPAGKITRYLYGLTYLPFDVKMAVIEAQKGIARPTANRILE
                     YCFAYNPASKTYTLQVTRIVGIITIFFLVLIMIILLARRRKK"
     gene            43598..45217
                     /locus_tag="PKK03_00210"
     CDS             43598..45217
                     /locus_tag="PKK03_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNX82838.1"
                     /translation="MGNETQPSYLEYQGRHKGLAGWLLSTDHKRIGLLYLYSIVVFFF
                     VAAFLGLLMRIELIAPGKTIMEPQTYNGLFTIHGIIMIFVIVIPGLAAVFGNFFLPIL
                     IGARDVAFPKLNLFSWYLYITGAVLGVMSQFMGSGPPDTGWTFYVPYSVESATSVIWA
                     LTAAFILGFSSILTGLNFIVTIHRMRAPGMKWFRMPLFPWALYATAWIQVLATPIVGI
                     TLLMVIAERVLNIGFFDPALGGDPVLFQHLFWIYSHPAVYIMILPAMGVVTEIFPTFS
                     QKPVFGYGAIVVSSLAIAFVGYFVWGHHMFTSGMSYYARWFFSFLTFIVAVPSAIKVF
                     NWISTMYGGSIDLKPPLLYAISFIFLFMIGGFTGLVLGSLATNVQTHDTAFVVAHFHY
                     IIFGGMGFAFFAGMHYWYPKMFGKMYNNRVANIAWGFNFVGFNLLYFPHFIIGIQGMP
                     RRSYDYLPQFHTGHFLSTIGAFILIAGLLLMLYNFIRAGRRGEKAPANPWHGVTLEWK
                     IPSPPPHENFDEIPEITSRPYFFGSKEKTNQ"
     gene            45224..45838
                     /locus_tag="PKK03_00215"
     CDS             45224..45838
                     /locus_tag="PKK03_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HNX82839.1"
                     /translation="MTVHPTHTEHIDSETGKLGMWIFLFTELFLFGGLFLVYAIFRAK
                     YSPEFHHAAEELNAFIGTINTVFLLVSSMTVAGALTAMQKGDRRMTLLLLTVTIMLAG
                     LFMVNKYFEWSHKFEYGIFPGSPVLKNMPHGELLFFGLYYMMTGLHALHVLVGMVLLS
                     INVVKVKAGTVNSERFLMLENSALYWHLVDLIWIFLFPLLYLIT"
     gene            45846..46130
                     /locus_tag="PKK03_00220"
     CDS             45846..46130
                     /locus_tag="PKK03_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNX82840.1"
                     /translation="MRDEKNHISTYRSLVTVLVILLALTTISVLITGIHLGPVTVAVA
                     LLIASVKVAIVITQFMHLKFENLFLKLAVSGVFTLFVLVIVITFIDYFFR"
     gene            46142..47089
                     /gene="coxB"
                     /locus_tag="PKK03_00225"
     CDS             46142..47089
                     /gene="coxB"
                     /locus_tag="PKK03_00225"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNX82841.1"
                     /translation="MFTGASNLAEGVDKAFLFIFIIAFIFIIGISAFMIWTVIHYSRK
                     KGREAKQFSGSMKMEVLWTVIPTILVMLMFWIGWKGFAPMRKVPADAMVVTAIGRMWE
                     WEFVYSDSLRSKELVLPINKPVKLDLVAEDVNHSLFIPAFRVKEDVIPGYDNWLWFTP
                     NYIGDYEILCTEYCGLLHSAMVSKTRIVEQEEFDRWYEELKVASFMPEHEGLKLLRST
                     GCLACHSLDGSKLVGPSFAGLYGSQREVVSGGSAKSVTADEAYITRSIYDPNGEIVEG
                     YARNLMQSYSELLTEEQVGIITEYLRELVEGDGQKEADL"
     gene            47109..47969
                     /locus_tag="PKK03_00230"
     CDS             47109..47969
                     /locus_tag="PKK03_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HNX82842.1"
                     /translation="MKTTRQPVCDIAELSRWKLSLAVAFSAVTGYLLFSGGDPGGAVL
                     TAAGVFLLSAGASALNQYTERESDALMTRTAGRPLPSGRMAPPAAMLAAAVMLAAGIA
                     FLAGGGTWPVLLGLLNVVLYNVIYTGLKKKTTLAIIPGALVGAVPPLIGYTAAGGAIA
                     DTRIILFALFMFLWQMPHFWLLLVRYGEEYEKAGIKTLHPVMSDKAIMRLVTGWITGS
                     SLLLWILTAIFMPFSRTASIILLLLNAAFILGFARITAIGSGRTGPEYTFMAFNSFSV
                     LVMVILIAFA"
     gene            48078..49640
                     /locus_tag="PKK03_00235"
     CDS             48078..49640
                     /locus_tag="PKK03_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNX82843.1"
                     /translation="MKKTLSISLMLMVCIATWSQPLKSDEIDKLVNRTLETFNVPGIA
                     VAVVKDDQVVHMKGYGVISITTGKKTDENTLFGIASNSKAFTSAALGILVDEGRITWE
                     TKVIDIIPEFRLYNSYVTEDFNIKDLLTHRSGMGLGAGDLMLWPDSSAFTTEEIIHNL
                     RYLKQTSSFRTKYDYDNLLYLVAGEVVRRVSGQGWDEFVESRIMKPLGMTESAASLNR
                     VRDRSNMIDAHVPVEGVLQVVPMYASTLLDPAGGIMSSVADMSKWVMMQLNRGKYGEG
                     NSKQMLSERVHREMWTPQTIIPVRNPGPYRSHFSAYGLGWGLTDVAGYLQASHTGGLA
                     GIVTQVTLIPELKLGIIVFTNQQEGSAFTAITNTIKDGYLGVKGNDWVKTLKENVDRN
                     RDEAKKITDEIWAKIEAQRLKSASAGSNAIYTGTYNDVWFGDIIISDEGGKLRFRSVK
                     SPKMRGEMLYYTGNTFVVKWDDRSMDADAWAVFTLDKEGKAEAFSMDAISPLTDFSYD
                     FQDLSPKRKEVR"
     gene            49637..51196
                     /locus_tag="PKK03_00240"
     CDS             49637..51196
                     /locus_tag="PKK03_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNX82844.1"
                     /translation="MRRAVSLILLLALAVAVSGQPLSSKKIDRLTTRVIKEFNVPGVA
                     VAVVKDGKVIHMKGYGVRSIASGQKTDEHTLFAIASNTKAFTAATLGILVDEGRLRWD
                     TKVTEIIPEFRLYNSYVTEDFFIKDLLTHRSGMGEGAGDLMGWPDSAMFTADEIIHNL
                     RYLKQASSFRTRYDYDNLLYIVAGEVVKRVSGQSWEEFAESRIMKPLGMNESAASFRR
                     IKDLTNIIDAHVMVDGRLKVVPKQEAKRHNSVGGIYSNVSDMSKWVILQLNSGRHGSS
                     AGEKIFSVEAHREMWTPQTVKTVSTSGSYRSNFAFYGLGWNLTDVNGYLQVYHTGSHA
                     GIETRVTMLPELSLGIIVFTNQQVPEAHEAITNSIIDGYLGIRGNDHIMKLREKVERA
                     QAMARKITDEIWATVEEKRKESSPGPGTGSFAGTYSDAWFGDIIISAEDGRLRIRAEK
                     SPRMRGRMFHYTANTFIVKWDDRSMDADAFAVFTLDREGKPVSFTMEAISPLTDFSYD
                     FQDLCLVRREK"
     gene            51261..52349
                     /locus_tag="PKK03_00245"
     CDS             51261..52349
                     /locus_tag="PKK03_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HNX82845.1"
                     /translation="MTFIHLSVWISALIGVIIIAWIRSFDIYEKETFIAMLWAFLAGG
                     ITSVIVALGAYEILRIFGLDDEVISNTLGSLLVIGPVEEFAKLIGLVVVYSLIRKQFN
                     ELTDGIIYMSCVALGFSIIENYFYANSGENSQYLLVYRAFISTPAHISFSVIIGYAWY
                     RYKKENKPFGTVIVALLVASLLHGVFDALAFSPWFNFMLLVYLWLIIRQSLRLVQYTN
                     VISPFRPGFEALLEAPSGETADGIECPNCGSSAPKELFRNSFFTACRCDNCGYHIASR
                     NDIRKIFRIFAPEYKRLAKKLVPARFSDGRTVMSVYGSAFFGNSGNPGFFRVSDVAGR
                     LQAINDDLLDRFRKRSFISANLLKRFFE"
     gene            52361..53494
                     /gene="nagA"
                     /locus_tag="PKK03_00250"
     CDS             52361..53494
                     /gene="nagA"
                     /locus_tag="PKK03_00250"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HNX82846.1"
                     /translation="MSYRRFRMYTLFYNARFFTGTGLVDGKELIVRDNRIEGFADQGS
                     VSADAHRIDCGGNLVVAGLIDLQIAGGGGYLFSSNPAPEALDMIIRSIVSAGTTGFLL
                     ALPTNSREVYRAAFLTVSGCTNPAVMGLHMEGPFISNARRGAHAPELIMKPVPENVTS
                     LLDESGGTIRMMTVAPEICTPEVITLLRERGITVAAGHSNATFREAMAGFEMGIETTT
                     HLFNAMSPLHHRDPGLPGAAFLSETACASIIADGIHVDYAMVRIASKIMKERLFLVSD
                     AVEENDRGAYRHVKKSDRFTLPDGTLSGAKLTMLDAVRNSINRAGIEKYEAFRMATLY
                     PARLIRAGDRGMIEPGARADLVIVDNEMKLRGVCLNGITEFKK"
     gene            53499..54449
                     /locus_tag="PKK03_00255"
     CDS             53499..54449
                     /locus_tag="PKK03_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNX82847.1"
                     /translation="MALLGIDLGGTKVAFALFDRGGNIRAREVLPLDDRRGGEVGKLI
                     AVNAALKLAAAEGEGDPVEAIGISVPGISRRTKGSVWAPNIQGWDDYPLLKEVTTVTG
                     RIPVIIESDRSCYISGEIWKGNARGCRDAIFLAVGTGIGAGIAVNGGLLHGADDIAGA
                     IGWMALSRPFRSKYTICGNFEYFASGEGIARQAAEYLKKHERYQGDLRKGPGAKVTTH
                     DVFAAREKGDVAASAIIDLAVEYWGMAAANLVSLFNPEKIIFGGGVFGPAVELIPAIY
                     QEAEKWAQPISIRQVSFEPSALGGDAGVYGAGYLAMKQIG"
     gene            54464..55627
                     /locus_tag="PKK03_00260"
     CDS             54464..55627
                     /locus_tag="PKK03_00260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX82848.1"
                     /translation="MKGTYNRQLVFWTACLGMLLFGIGLIMLGSVLPDLRVRHSLDAV
                     EAGTLLSVLPIGIIFGSLLFGPVCDRYGYRVLLAVSALLMFAGFEGLAFTRTAWILTL
                     SVFLFGLGGGAINGATNAVVADISTTGKGAALSILGVFFGIGALGMPLLLSLLKATFE
                     FQVIVAATGMLALVAAVLFAVVRFPAAKQAEGISMKQVGALFRDVTLLLIAFFLFFQS
                     SFEGLINNWTTTYLMERVAAPQEAALMGLSAYVAGMTVMRLLIGSVLRKMPEKQLLYI
                     SFGITLAALLLFSLSRSVIPAAAGLFMLGAGLAAGFPTMLGIVGNRYPGLSGTAFSFV
                     LVIGLLGNMLVNYSMGIVAENAGIKHLTTFTYAELAILVVMATAIFGRLKMNK"
     gene            55654..56415
                     /locus_tag="PKK03_00265"
     CDS             55654..56415
                     /locus_tag="PKK03_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HNX82849.1"
                     /translation="MLALEWLNNARNVMNNIEETQMENIRKAAEVMADTIECGRWVHT
                     FGCGHATLPIEEMYPRIGGFVGFHPMIEIPLSFFTHITGEMGVHQFVFLERVEGYGAE
                     IMKGYTFDTRDTMWLFSHSGINNVNIDIALESKKRGMKVVVFGSAAAAKGKKTRHSSG
                     KTIFDIADIVVDTCAPLGDASVELKNHYDRIGPVSTMAFVTCVWMTITTVAEILADRG
                     VKLYIHPSHNVPGDNTARERLDEALAEYKRRVAGV"
     gene            56428..57456
                     /locus_tag="PKK03_00270"
     CDS             56428..57456
                     /locus_tag="PKK03_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNX82850.1"
                     /translation="MKPLDRTIGFIRGTAVDRVPFHPILMRFAACHAGVRYRDFCLSP
                     AHKCIANIRCAEDFRSDWVNTMSDPWAEAEAFGTVLSYPDDDLPKVERHAIEEISDID
                     RMTVLRPGDHRRMLARVEEIEIYRRDTAGVLFICGWVEGPLAAWCDIRDINMAMTDLY
                     EYPAEVHKALDIITESAMAFITPQVSAGAHCIGIGDSVCSLISPELYDEFCFEREKNL
                     VEHIHSLGALAKIHICGNISAILPRVIGTGADIIDIDHRTGPVTEVLGLLMPGQVFSG
                     KSDPVSVVQDGDTESIRSSCRSFFDEAGERAILSAGCEVTPGTPAENLRFFSGMAGEL
                     LPAAGRKV"
     gene            57514..58386
                     /locus_tag="PKK03_00275"
     CDS             57514..58386
                     /locus_tag="PKK03_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNX82851.1"
                     /translation="MSRNVALVLSSGGARGFAHIGAIKVLEDNGYTITSVAGTSMGAL
                     VGGIYAAGRLKEFEEWVSELDVMEVLKLTDISISSKGFVKGNRVIEKIREIVHDHNIE
                     ELRIPYCAVATDVIRGRERIFTSGSMFNAIRASISIPTFFQPFRMDNDYYVDGGLVNP
                     IPINRVKRIKGDLLAVVNVNADIPHVSIGTADQDENRAKGYLRRIRNINAHADRHIPK
                     NKKDDIGILNLNHRSISIMLNQIASLTLMNNHVDIMVQVSKESYGIFDFYKAAEIIRE
                     GEHATALALGVQLP"
     gene            58477..59376
                     /locus_tag="PKK03_00280"
     CDS             58477..59376
                     /locus_tag="PKK03_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82852.1"
                     /translation="MKRSYLMLCLVLLITAPAMFGQKPIKVLEDSIQFGNYLYPGFNV
                     TIPEANYDKILKDWIKTQESGTKSKVQTEGGEMTIFGAIVKEVSPAPVNIYSRIMNED
                     TLSRMLVCIELKKDQYVEAASGDIQLTSAKNYLKEFAKRQYMDFIKEELAAEEKILRE
                     LKQDLGSLENSKARTQRTARNNRNTVNDEQEKLLVKHNELSLLSNEIINKNNEMIAMP
                     VGPGRDAMATQVKELEKRRKKLQKEISKGESRINKARSAIDQADRAIPRNDDEQAVMK
                     SKIDAQQAVVQTFIDKLNNVKLY"
     gene            59425..59751
                     /locus_tag="PKK03_00285"
     CDS             59425..59751
                     /locus_tag="PKK03_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82853.1"
                     /translation="MENQEKKETYQQKIQEQLDEWRADIDRLKEKAKNATAESKLKYQ
                     ENIDKLEVKMDEGKSKLKDLKESSAEAWDAVKDGADSIWDTMKATFAEVKDRLRDKDD
                     DDDKKA"
     gene            59906..60316
                     /locus_tag="PKK03_00290"
     CDS             59906..60316
                     /locus_tag="PKK03_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82854.1"
                     /translation="MKKFVTIGIVVLLIAAAVIFWWRHYFVFGEGVKAGDLNYLVRKG
                     YVFKTWEGKLIQTGFKTPTPGAIQSNEFEFSITNDSLATVLERASGKFVELRYKEYIA
                     PLPWRGMSKYVVIEILDIKDSEQEQQTLIPYQAI"
     gene            60339..61097
                     /locus_tag="PKK03_00295"
     CDS             60339..61097
                     /locus_tag="PKK03_00295"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HNX82855.1"
                     /translation="MTRTRTAALVVLLIMLSGCAELLNTLKTISAVKQLTEADIVAGL
                     REALSVGARNAAGRLASQDGYWGDPAVRIPLPDEAKVIVENIGRIPGGQQLIDNVVLS
                     INRAAEDAAREVAPIFVNSVTQMTITDGYNILHGADNAATVYLRNTTWNGLYDLYKPK
                     ISASTGKEIVAGVSAQDSWNTLTGKWNQVANSVAGRLAGFKPVNTDLDDFLTRKALEG
                     VFLKLEGEELKIRKEVSARVTPILREVFGTLDQQ"
     gene            61176..62087
                     /locus_tag="PKK03_00300"
     CDS             61176..62087
                     /locus_tag="PKK03_00300"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HNX82856.1"
                     /translation="MRKITIVLSLALALTGINPAASGQDADTTQGWHKSGVFSLNMSQ
                     ANFTNWAAGGQNSLALNGLVNLGANYRMGKSAWDNALIIGYGKMVQKGNDLGWMKTDD
                     RIDFQSKYGRKATEKWFYSGLMSFKTQMDNGYNYPDTDNKISGLLSPAYLLFSLGMDY
                     KPNPNFSVFLSPLTSKNTIVMDDDLSMAGAFGVEPGKNFRPELGAYANIAYKKDEIVK
                     NVNFMTKLDLFTNYLKNPQNIDISWEALLVLKVNEFISATVNTLLLYDDDILIKIDEG
                     SEGEPVLGKRAQFKEVIGVGFTYKFIK"
     gene            62112..62753
                     /locus_tag="PKK03_00305"
     CDS             62112..62753
                     /locus_tag="PKK03_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNX82857.1"
                     /translation="MKSITLFAIMTLITGILTGQAVQDIKLNAPDMERGLTIMKAFEK
                     RASATSFSSRDLSLQDLSDLLWASNGINRPESGKRTAPTAVNAQDVDIYVLLRSGAYL
                     YDHKTHTLKGIAAGDNRKLVSDRQTTFANAPVMLVLVSDVSRFRGDDDARKLSWGAMD
                     AGIVSQNVSLFCAGAGLVTRPRASMDIEGLKKLLQLNGTQHPLLNNPVGYPNE"
     gene            62766..63386
                     /gene="upp"
                     /locus_tag="PKK03_00310"
     CDS             62766..63386
                     /gene="upp"
                     /locus_tag="PKK03_00310"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNX82858.1"
                     /translation="MLKLINHPLITHKLSLIRRKETGTRDFRQNISEIAGLMAYEITR
                     DLPLREIVIDTPLSSCSTAELSREIILVPVLRAGLGMVDGITALITTARVGHIGLVRD
                     HETLAPMEYYAKFPADISEAVVMVLDPMLATGGSASAAISAVKKRGAVTIRLVCIVGA
                     PEGVTRVEKDHPDVDIYLAALDSHLNENGYIVPGLGDAGDRLFGTV"
     gene            63426..64679
                     /locus_tag="PKK03_00315"
     CDS             63426..64679
                     /locus_tag="PKK03_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HNX82859.1"
                     /translation="MGLKRRLIIMNFLQFFIWGSWLLTIGAYWFQNKNWSGTDFGAIF
                     STMGLASLFMPAIAGIIADRWINAEKLYGIFHIAGAVLLSLIPAINNPDTFFWVLLLT
                     MMFYMPTLALAITVAYSSMKNSGMDIIRDYPPVRVWGTIGFIVALWVVSLLGFETSAL
                     QFYVAAAASLILGLYAFTLPKCPPLAKGHKKKLIEELGLNAFKLFKSTKMALFFIFAM
                     LLGGALQLTNAYGDTFLHDFAKIPAYKDLLAVKYPAIIMSVSQISETLFILTIPFFLK
                     RFGIKKVMLMSMVAWVLRFGLFGVGNPAEGLWMIILSCIIYGMAFDFFNISGSLFVET
                     QSTPDIRASAQGLFMMMTNGFGAFLGSRISGIVIDKYFILEGGAFDWKGIWFAFAGYA
                     LVVAVLFAIFFRHKHVPEETASVSH"
     gene            complement(64842..65738)
                     /gene="zupT"
                     /locus_tag="PKK03_00320"
     CDS             complement(64842..65738)
                     /gene="zupT"
                     /locus_tag="PKK03_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HNX82860.1"
                     /translation="MDSSRLLFAFGITLFAGLATGIGGLIAFFAKRTKTTFLAFSLGF
                     SAGVMIFISFTEILTGAGDIMRQNYDANTAAWLTFIAFVAGIGLSAAIDKILPSEGNP
                     HELKRVEQMHPESRDPDKPECQGRKNQNRGGHDTGNQNRRGHSIRDRKLMRIGTFTAI
                     AIGVHNFPEGIATFMSAMSDVTVGISIAVAVAIHNVPEGIAVSVPVYYATGSRRKALA
                     WSVLSGFSEPIGALAGYFILTLFNTGISLGYVFAMVAGIMVYISFDELLPAAHKYGKH
                     HVAIYGLISGMIMIGISLILLG"
     gene            65838..66452
                     /locus_tag="PKK03_00325"
     CDS             65838..66452
                     /locus_tag="PKK03_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNX82861.1"
                     /translation="MILQLTMNLWDYYRILGVKQNATDDEIRKAYRKKAMEYHPDRNS
                     SPDSQEMFIRITEAYEYLISHPYRRNITEEEVRRNYQAWIDYRREEARKRAEAYARAS
                     YAEFRKSPIYKSTTVIDGTIVFLGLILATMVIFTTIYGYIYRMKVADSPKEEPSITLA
                     AVSLIIGISYMIISVIYLSAWIAQQKKKRRISEDGAQKEDKESV"
     gene            66418..66930
                     /locus_tag="PKK03_00330"
     CDS             66418..66930
                     /locus_tag="PKK03_00330"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNX82862.1"
                     /translation="MEHRKKIRNPFDADQNMCFGCGPKNHVGLKLLFEEDDEKVYASW
                     VPDRQFQGYINVLHGGIIATLLDEVSAWCVYLKACTSGVTSSMTVRYLRPVHISKGTV
                     TIEAMITKKGEKNALISAALHDGEGKLCAEAELDYFIYPEKIARDRYHYPGREAFMYQ
                     SVPPGSPSGQ"
     gene            complement(66887..67912)
                     /gene="tsaD"
                     /locus_tag="PKK03_00335"
     CDS             complement(66887..67912)
                     /gene="tsaD"
                     /locus_tag="PKK03_00335"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNX82863.1"
                     /translation="MAPQITILAIESSCDDTSAAVICDGFVLSNLIANQDVHKAYGGV
                     VPELASRAHQANIVPVVDQALARAGKRADEVSAVAFTQGPGLLGSLLVGTSFAKGFAL
                     ARNIPLIAVNHLQAHVMSHFAMEHGKENRIPQFPFLCLLVSGGHTQLIVIRDHLVMEL
                     IGNTIDDAAGEAFDKCAKVMGLPYPGGPVIDRLASVGNPDAFRFAKPRIQGLNFSFSG
                     LKTSFLYFLRDRVAEDPGFIGKNLNDLAASLQRTIIEILIGKLVRASRETGIREIGIA
                     GGVSANGGLRRAATREAEKRGWNLFLPEFRYTTDNAAMIGITGYYKYLRAEFTDHSVI
                     PVARIDT"
     gene            67965..72488
                     /locus_tag="PKK03_00340"
     CDS             67965..72488
                     /locus_tag="PKK03_00340"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNX82864.1"
                     /translation="MAKIIKKIVRVLLIIAAVLVVVPLILFLLLQAPRIQTWTVSKVT
                     NAVSGKTGAEISIGRVSYSLWHEIVLDDVLFRDLNGDTIVAARKVDLRLREIRPSHRI
                     FKFGTIDVHEPDFRMIKDTAGVMNLTAYLRSLKSDRERDSTGGIDIRFADIDVLNGSF
                     TLADMSDTIGVVAGSVNYMNMHIRHITGKVRDLRIIPDSVSMVIRGLAFHEAGGFVSR
                     SLDMNMAVTESSLYFRDIDLLTDSSAISAEKILLMPREEDSWKDFINDVRIDFLFNNS
                     IVNTSDISYFVRPLAGISEEVSFSGRVAGTVAELKGKNIRIDYEASTRLRFDFDISGL
                     PEINDSYLFISFTDMKTRAADIERFTVPGRKPLRLPEVAHDLGLVTYKGSFTGFTTDF
                     VSFGRITTERGTFDTDLSLKPDGRNTFGFRGSLRTRAVDLGYMAKNSEMFGGLWMHAE
                     VDGTMKSFRHLSANINGVIDSVEVNKYLYRNISVSGTYADRIWDGSVEVNEPNIEMSL
                     LGRFDLEKSQPEFDFTMNLADADLHTLNLVKKDSVFRATALVTANFRGNSTENLEGDL
                     RLINSTLENSNGKLNIYDFLVSSEIVSGEPVLRLRSDFADADVRGQYSVDAIKSSVSE
                     IMAGLFPSRFNKPVAKKGESTPEAMFSLDARIKRIDRLNEFLGTGLGISEGTHVTGHF
                     WSDRPEVIAGIKADAVTYMGIRMGRLNMTGSLKDGSLKLDMTADTVMLPDKSELGNFA
                     LGATSCNDTTDLGMNWDNHDNGRTLGEVKARGFFSHGVGNLPSLTVAVLPTGLTVNSS
                     QWNISPARIVIDSVSTFFDNILVSSRTRYFSLGGSLSKDPEEKLTLAFEGLNMSYLNN
                     LLRGKQDETEEPTQMKFEGILSGNLTLSDVFNNLLFESRINVTDFRLNDNNYGLMTII
                     SEWDPRRKVAVINVGNDFAGRKLIDVSGTYSPSARVIDIAASAFDLPLDVLNPFLSSF
                     ASDIRGFGTGTIRLKGKPRQLEMTGAVMAHDASMKIDFLQTRYSFSDSVRFSPSGIIL
                     RNINIYDAQKNQGMINGMITHRAFKNFGVSLDINMDKMLVLNTRPKDNDSFYGTAYAS
                     GYAGIRGNDEKLVFNISARTAPNTEFYVPLNSSASVSDYPFIEFLKPAGETGEQEQPD
                     RQSSFVRQEEGSKMELNFDLDVTPEAEVQLILDETAGGVIRGKGEGLLNISLNSRGDM
                     RMAGDYVISDGDYLFTLGSILNKRFAVEEGGTVSWNGAIEDASLDIRALYKTKASLSE
                     IFGEEEFTELKAKMPVECILSLSGRLLNPAISFDISLPTADERTRELLRMAIDTEEEL
                     SRQFLYLLVMNSFYPDPSLYNSGTTTGTPVAQGGYAVSPIVGVTTTTEMLSNQLSNWL
                     SQISNDFDIGFSYRPGSELTDQEVELALSTQLLNDKVTVNGNVDVRGNQSNTTASNIS
                     GEFTVEVRLTDMLRFKVFNRSNHNLYYQVHPYTQGVGLFYRRDFSTLKDLFIKPEEKK
                     RKNIESEGETGQN"
     gene            72615..73298
                     /locus_tag="PKK03_00345"
     CDS             72615..73298
                     /locus_tag="PKK03_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNX82865.1"
                     /translation="MISAAILLQGTPATEAAEEATMSLADLYLAGGWVMYLITLLSFV
                     ALYIFIERYLVIKKASKGDANFMNRIKDYMYEGKVDAALQLCRKTNSPQARMVEKGIT
                     RLGRPLADVTAAIENVGRLEVHKLEKRFPTLATIAGAAPLMGFFGTVIGMVQAFYEMS
                     LAGNSLDISTLSGGIYTALITTVGGLILGILAYFGYNQLVVKVENVVFQLETTSTEFL
                     DILNEPVKQ"
BASE COUNT        18540 a        17369 c        19936 g        17526 t
ORIGIN      
        1 aataatttag taccataata aacccttcga ctgctcatgg acaaaatatt gatttataat
       61 gacaggcatg ccttccggga atggcttggc aagtgcgggg cagagagtga tggcatatgg
      121 atactcttta gcaagaatga aaaaattgtc acactttcag ccgcagaggc attggagaag
      181 gcgctctgtc acggatggac agatggtcag atacaatcac cggacgacat atgaggcaat
      241 atggcaaaat gaaatagcta aaaaatacag gaaagcactg gagctggacg ccggcaataa
      301 ggatatcaag aaattactcc ccggactcga atcaaaataa tctacctgaa taacaacgga
      361 caaaaatgaa aatctacctg agattaatag ttgcatctct catccttgca ttttcaattc
      421 ttgctgatgc ccagtctgac ttcaggcgag ggtacatcat cacgaatgat gatgacaccc
      481 tggtgggctt tattgatttc agaggctata caagcaacag tcagaaatgc ggtttcaagc
      541 ctgctgaaaa tgcacctcct tcagtattta aaccaggtga catcaaggca taccggataa
      601 tggatgataa atattatgtt tcaagaccgg ttgaaacaga agagggacaa aaggagatgt
      661 tcctcgagta tcttattaaa ggagaagcga gcatctatta ttaccgtgac aacaagggtg
      721 agcactacct tattgacaag cggggcggac cgctgacaga gctcccctac cataaggaag
      781 aggctattat cgacgaagtc ccgcgtttcc tggaatcaac aaaacacatc ggtcttctga
      841 aagcctatat gagtgattgc ccggcactct ttaaccggat tgaatcactg aaaaaaccgg
      901 atcatgacaa tctgatagcc ctggcaaaag actatcacca gttaatatgc aatgacaaca
      961 gttgtattgt atattccaag agaaaacctg cactgggagt ggctgctgaa ccagccgtca
     1021 ttttcaactg gttcaggtac gggaccggca tatacacagg aacaggcatt tcacttcatt
     1081 tctggttgcc gcgcagcagc gaaagactgt attttagaac cggatacatg tacaccagag
     1141 gagttcttta ccaacgacgt aatgatgaga tggagtggat aggtatcgaa cgttctatac
     1201 acaggattcc ggttcagttt gagtacctgg ttccggggag agtggtgcaa cccaggttta
     1261 attacggaat taatctgatt ggtacggatg attatgataa agcattcgat cagacggccg
     1321 gagcgggttt actggtgaag gtctacaacc agttatacat aagtggaggg gttgaagcag
     1381 attggactcc tatcctgtta tcactgatga tggattttga catcgagttg gtatctctct
     1441 cgttaaactt cggtttatat tacaggtttt agtatcacag gtttacagac tgaaagtacg
     1501 gaataaaatg agtaacagat tcattatcca tcgtggtgag ctggtctttg aggaagattg
     1561 tctgctcatc aaagacgggc agcctgcctg gttcaggatt gccagcattg tcagcctgat
     1621 agccataatg ctatatggag tactggttat catcaagtac ttcaggggcg atgactcttc
     1681 agatctttgg atcggtgtaa cgataatcag cctgggaatt ccggccatga ttgttcagtc
     1741 gaaaatttct tatgacagca agatagaata caagcacata cgtagtatca aaattcgaag
     1801 gaatcttaca gaccaactgc ttgcggatat tttgctcaac aaccgtaaaa agcgcaggat
     1861 agtgcttgac agtgaagatc tcgggcggtt cgggagagat caccttgaca agctgattga
     1921 agaattcaat gacaaacagg tgcagacaat tcctgcctga agtccgcaat aatttgtacc
     1981 ttgcacatga atcagaccag gttgtgaaat acctcaacaa cagaatctcc aaacattcat
     2041 agcatgaaag gaataaatcc gctacttgca ttatttgccc tgataatttt tacagccttc
     2101 agttcatgtg cctcgatacc caaaggtgct acagctgtca gcccatttga taaagaacgc
     2161 tacctcggta aatggtacga gattgccagg cttgatttca aatttgagcg gaacctgaac
     2221 aacaccaccg ctgaatattc ccttaacggg aacggaacga taaaggttga caaccaggga
     2281 tacaacacag tcagggaaga acggacccag gccattggca aggcgaagtt tgcaggtgac
     2341 gacaaggttg ccatgcttaa ggtttcattc tttggtcctt tctattcagg atacaatgtc
     2401 atagctctgg atgatgaata caggtatgcc ctggtagcag gagagagcct gaagtatatg
     2461 tggatactct cacgcgaaac caccatccct gacgaaatca aaacgaagta tctgaagctg
     2521 gcagaggaaa taggttacaa cacgtctgag cttatttggg tagagcataa caactccaga
     2581 taataggatc atcatggaat tcgacaggca aagggtcact gaaactgcca ggcgagagat
     2641 tatggccggc aaatccaagg aggaggtctt cagggatata tacgcctctt tcaatgatcc
     2701 gaggttgcat gaaccaatcg caaggatagt tcagtatatc ccggaaccgg cgagaatcag
     2761 gaaatacggg ataatcaata ccatattcat gtttctgctt gccgccatct gtattgcact
     2821 gataatcagg tcacaataca ctggcatggt tataccggcc atcctgctgt atctcgtctc
     2881 cacccgccag acaaggtact atcaatggat aacgtttgtc ggcggactgt tgttaatcat
     2941 ctccctgaca atggccatta ccggctggcc ggaagtaaaa gagaatcctt tgctgacgct
     3001 tggcctggca actctgacgg gagtgctgtt cattctgttc gggatactca tgcccaggct
     3061 actcactccc ggctatagag cggttgaaga gacgacaact gacagtgacg ggcggacggt
     3121 tacgcataaa aagttgattt tctgacatat attcaatcaa caaccaccta tttcagactc
     3181 ataccgcagg aacatcatga aaaaacttga agatatactg cttcttggca atccgcttct
     3241 gtatgaagtg agtgaaccgg ttcttgagag tgaaagagat cttctgccag gatggaaggc
     3301 ggacctccac ggcgtaatgg aggaggtgcg ggcgaagtat cacttcggcc ggggcatagc
     3361 agcgcctcag ctcggaatta tgaagagggt gatatacatc aatacaggaa agcccctgct
     3421 catcatcaac cctgagcttc atgacctcgg cgatgagatg atggaactgt gggatgactg
     3481 catgtgcctg cccaacctgc tggtgaaggt aagacgtaac cgcagcctga cactgagata
     3541 cctcgacgag cgctgggaga acagcgaatg gcatgttacc gatgacctcg ccgagctggt
     3601 tcagcatgag tatgatcatc tgaacggcgt tctttgcaca atgcgggcca tcgacgggaa
     3661 gtcgttcagg tggaaaacgt agagagattt gagatttgcg atttgagatt tgagaattga
     3721 gaattgagat tgcaggtgga tacagaagct gccatgtaag tgacaactga actcacggcg
     3781 ataatggtta tctttgatta ctaaaacagg ataacctatg accgcgacaa ccaccaccac
     3841 cgcagcatcc gcagcagcta cagctacagc tacagcaaca gttatcggtg ccaccggcct
     3901 gatcggcggt cacctggcag agctccttac aaaagatgac tacttcaaga aagtacggct
     3961 gataaccagg agaatgcatg aaaacacccc gccgggtgct gaggtggtta tcatcgactt
     4021 taatgatccg gttgctttca ggtcggctgt tgctggcagt gatgcagtat tctgtgccat
     4081 cggcaccacc cggaagaagg taaagggcga catggatgcc taccggaagg ttgaccatga
     4141 cattcccgtg aacaccgccc ggcactgcca ggaagccggt tgccggcact ttgcgcttgt
     4201 ctcctcggtc ggggcatcag caaagagcgg caatttctac ctgaagttca agggtgaggt
     4261 tgaggaggct gttgcgctga tgagcatccc ctccgtttcc gttttccggc cttccatgct
     4321 aatgggcaag cggaatgagt tccgcctggc ggaggagatc acaaagattt tcgctcctcc
     4381 cctctccttt cttttcccgg caaaatacag gcccataaaa ggtcatgatg tggcacaggc
     4441 catgatcgct gccgcgaaaa gggcagagcc gggattcagg gtatatcact tcaacgagat
     4501 gaaggctctc ataaaggaca gggaagtctg accacatacc ttaacgcgaa tattatccac
     4561 accaatggaa gtaaatatca gaagagcggt tgaagaggat ttcccggcaa tcctgaaact
     4621 tataaaagag ctggctgagt atgagcggtc tcctgaagcc gtgaccaata ctgtggagca
     4681 gatgcgccgc gaacagaacc acttccgctg tttcgtggcg gataatgcag aggatggcat
     4741 tatgggcatg gcgctttact ttttcgccta tttcacctgg gtggggaaat cgatctacct
     4801 cgaggatatc attgtcagca agctgttccg taaccggaaa ataggtgcag ccctcatgca
     4861 gagggttatg caggaagccc gggacgagaa ctgcaagaga gtccggtggc aggtgctcga
     4921 ctggaatgag ccggccatcg gtttctaccg gaagtgcggc gccgagatca gcggcgactg
     4981 gctgaactgc acctttgacg aaaaggggat ctctctcttc aacgaaatga tgtcatgatg
     5041 agaattgata ttaggcatca ggcaggcgga tttgccggag cactgagaat atactccggc
     5101 agaaccatga aggtcacgct gctgctatca gtcatattca ttcttaaaac tataaccagc
     5161 tgtattgatc cggcggaatg ggattttgag ttaagtttca acaaaattac catcactaac
     5221 ctcgacaatt ccggatttta tcccacggag accaacagcg atatcatgta tgccccggct
     5281 gtggctttca ctgtgaccct ttccgacgag acaatcctgg cttcaggcgt taacaggcga
     5341 agcaataaca gctttaccgg attcacccct gcatacgcca tgtctccgga acccaggtat
     5401 tatccgctga accggatcac aggaatcagc attgtcacac ttgaggctat gtcacctgaa
     5461 attcctgccg gcaccgatgt cacaggtctg ttcgcagcct gcgtgccccg attcaatgca
     5521 cctggtttcc tttacattga ttcctcggag cttctatcag ccatagaccg ggacttttac
     5581 cccgatgaac cgtcggtcac ctttcagttg ttctgccggg aggaaattgc aggcgacaag
     5641 gcgcaatttg aaatcaacat aaccctgtcg gacgaaagca cactgtctgc aaccaccaac
     5701 ctgataaccc ttgtcaggaa cgaatagcac atatgcaatg agacgctatg ccaaaataat
     5761 gatgttcctt ctgcctgcgg ttgccgtttc ttccctcctt ccggcccagg gccggttcat
     5821 cgaaaagaaa gaggcatcac tctggtttgg cgtgagggat ataacccgcg acagcctggc
     5881 taacggcgcc aacctggatt ttctgaacta tcttcactcc tcggatgaga tggctgaata
     5941 ccagtatctc ggtctttcgg cacatctctg gttccggggc aactgggagg cggattttaa
     6001 ggtggcgatg tatgatgatt ttgcgcctac cagcctgaac attaaggcta tatggatgcc
     6061 atataaatat ctggggttaa cggccggatt ctacagctat cctcagctga tgaatgagtt
     6121 taatatgttt caccgccttt ccgacgaagg gtggtatggt gacacggaca gcaacttcag
     6181 gcaaaggcgg gttcatgaaa caggaatcat ggcaggggtg gcagtgccgg tcagttaccg
     6241 tttcatccac ctcaaccttt gtctgaatgg cggtatcagc acgctttcaa aattccgtga
     6301 gaaagtgaca cagaaaatga tcaatggcaa catgaggcgt gagatcaggt acagtacgaa
     6361 aaactccccg gcgctcttct tcttccccga agcagaactg ggccttgact gtttcaggat
     6421 cggcagttca acactgggtg tcagaggcca tgccagctgg tttacagtta aaaggtctgt
     6481 tgactacaag atgacaatat gggaatggac ctatgataat ccaatgcagg aggatgttgc
     6541 caatccggca cacagtttct ctaaatttga atttgatgca ggtctttacc tgacctggtg
     6601 aagtatgttt cagagcaccc ggattgcatc gtcatatcgc agagattttg cttttaactt
     6661 aatagttcta attttgtttt agactaaaac ctgtcccaat gagcgaactg atcaacaatt
     6721 catcggaaag aaagaagagg cttaaaacac ttatactcag gcttcaccag ggcgaagccg
     6781 aagaaaaggt ccgggaggat cttttaagca ccctcagcca gatcccatac ggagaggttg
     6841 ttgaggttga gcaggagctg atcagcgaag gtctcccgga ggaagaggtg ctgaggctct
     6901 gcgatgccca ctcggcagtg cttcacggca acgtcgatct ctcggcggca aaaaagatac
     6961 cggcaggaca cccggttgat gtgctcatca atgaaaacaa ggagttgagg aaggttgttg
     7021 ccgagataga gaaccttata tcagccatca gctatgattc aggtccggta aaggacacag
     7081 aaaaattcag gctgaccggc ctgttcaaca gcctgtggga tgttgacaag cattaccagc
     7141 gcaaggaata cctcctcttc ccgtacctcg agaggcaggg gatcaccggt ccgcccaagg
     7201 ttatgtgggg caagcatgat gagatcaggg aactgataaa ggggggcatc gagctccttc
     7261 gcagcggcga gatcacacgt gaggaactgg tagcatcagc tgagattgtg cttcttccgg
     7321 ctgcacaggg ggtgaaagat atgacaacaa aggaggagga gatactcttc ccgatggcgc
     7381 ttgacaacct caacgaggct gactggtatg ccattcagaa gcagtcgctt gagatagggt
     7441 actgccttta tgacccgagg gtaacctgga aacctgaatg ggcgcaggaa gacatcctcc
     7501 atgagatgaa caagaatgca gacacagttc agctaccctc gggcagcttc acaattgaag
     7561 agttgctggc catacttaac aggctgccga tagacatgac cttcgtcgac cgcaatgaca
     7621 aggtaaagta tttctcgcag agcgaagaga ggatcttcca gcggaacagg gccatcctga
     7681 acagggatgt gaggcactgc catccgcctg ccagcgcaca catagtggat aagatcatcg
     7741 aggatttcag gtcgggcaag gcttcgaggg cccccttctg gatcaatatc ggcggaaaga
     7801 tgatctacat cgaatacttc gccctgcgaa acgataaggg agagtacctg ggcacgctgg
     7861 aggtgtcgca caatgttacc ccctacaggg agcttgaggg agagcaaagg atattatcat
     7921 attcaaaata accatggatc agaacaagat aatcataagt cccaagacta aggtgctggc
     7981 gctgattgaa gcctacccgc agcttgagaa tacgctgata agctatgtgc cggtgtttga
     8041 gaagctgaag aacccggttc tgaggaggac ggttgcgaag attgccaccc ttcagcaggc
     8101 ggcggtcatt ggcaacgtga aggttgagga gctcataaat gtgttgcgga aagaagtcgg
     8161 gcaggacctt atagccggag ctgctgagca ggagctgaat accgtcatgc cttcatggtt
     8221 tgatccggct ctggtgaagc aggagactga catcaggcct atgctggctg ccggcgaaca
     8281 accggttaac caggtgatgg ctgacctgaa cacgctggag gcgggtcaca tatacaaagt
     8341 aattgcgccg tttgttccgg cacctctgat agagaaggcc gccagcctga acatcgatca
     8401 ttggctcaaa caggaggctg atgagctatt caacgtctat ttcaggcgga gctgaccggt
     8461 cagggtattc atggaaataa aatcagatca tacggacaag gtgtactcat acctggcgct
     8521 gcggaaggcg gtgggctgga taggcatcct tcttcccatt gtgctggtgc tgggccatct
     8581 catcattttc gacggtgaga gtgcgcttac gaacatgagc gtttattatc atacaggtat
     8641 gcgcgatgtg tttgtgggtg caatctgtgc aattgcgctg ttcctgtttt tttacagggg
     8701 ttatgacaga tgggacaaca gagctgctga cctggcaggt ttatgcgccc ttggtgtggc
     8761 attttttccc actgtggagg aaggatcatg ggattgggtg gcatggattc atttcacttc
     8821 tgcagcctgt ttccttgtga tactggcgct gatttcgata ttccttttca cccgtggtga
     8881 gaagcatccc actgagatga agaagaagag gaacctgata tacagggcat gcggcatcgt
     8941 tatgctggca gctctggctt caatcgaggt ctttttcctg ttcttcgacg gggttaattc
     9001 cgattcaagg tttgtgctta ttgcagagac cataactctt atggcgttcg gcatctcgtg
     9061 gcttacgaaa ggcggaacgc tctatccgga taaaccaata aagaaaaacg atatggaaaa
     9121 cgatgaaaaa ctgatcaggg tctttgttgc ccctgagcct acagtcatgc tgctgcttga
     9181 gatgcttgaa gagacaggca taaagggact gataaagaat gactcgggac tcggatacct
     9241 cggagcagtg cctcaggtca tggatctgta tatcctggag gaggacctgg agaaggccac
     9301 tccccttatt aacgagttca aggaaaaaca caattccgaa aaggattcct aataacaact
     9361 cctgtatgaa aaagacattt ttacctgcag caatcatact ggtgctgatt gctgtctcgt
     9421 gcaacgtttc aactcaaaca tctgatatga tcattacaaa tgccaggatc tggacgggga
     9481 acgcggtaca gccttatgcg gaggctatgg cggtctccgg tgacactatt attgcaattg
     9541 gaagcaaccg cgaggtaatg aagtacaaat caggttccgg tgaagttatt gacctcgggg
     9601 gcaggtttgt tgccccggga tttattgact cgcacctgca tctgctccag ggaggatcaa
     9661 acctggcctc ggtgcagctt agggatgcgt caacacctga ggagtttatc agccgtataa
     9721 aggaatatgc cgcaacactg aagcccggaa catggattct cggtggcgac tgggacggga
     9781 ttggctggga gagcctcccg cagaaggact ggattgactc tgtaaccccg gataatcctg
     9841 tgtttgtgtc gcggcttgac ggacatatgg cgctggccaa ctcgctggcc atgaagcttg
     9901 caggtgttga ccgcaaggta aaggatgtct ccggcggcac aatcacacgg gacggaagcg
     9961 gtgagccgac gggtattttc aaggacaatg ccatggatct gatatctgtc aggattccgg
    10021 aacagtccga ggaggaggtt gacagggcca ttgttgcggc gatgcattac ctggcatcca
    10081 acggtgtcac ttctgcgcat gcagttgatg cagccgctta ttctgatgcc cttgaacggg
    10141 tacgtgcgcg gggcgaacag ataacccgcc tctattacct gcagacaatc agcaggtgga
    10201 aggatatgaa ggagcaggtt gagcgtgacg gacgcggtga caggtggatc agcaccggtg
    10261 gagtcaaggg attcgttgac ggctcgctgg gctcgcacac tgcggcgttc ctggagcctt
    10321 atgctgatct tcagacagat acaggcctct ttgtgaacag tgaggaggac ctctacaact
    10381 ggattctgga atccgacaaa gccgggctgc aggttgtgat tcatgcaatc ggtgacgagg
    10441 ccatcaattt ccttcttaac gcctatgaga aggtggctgc cgttaacggg gagcgtgaca
    10501 ggcggttcag gatcgagcac tcgcagcata ttgcacccgc ggatattgag cggtacggga
    10561 agctgaaggt gattgccagc atgcagccat atcatgccat cgatgacggc cgttgggctg
    10621 aggaatacat cggtccggaa cgggtgaaga caacctatgc attcaggtca ctgcttgaca
    10681 gcggggcaac ccttgccttc gggagcgact ggttcgttgc gccggcaaca ccgcttgaag
    10741 ggatatatgc cgccacgaca cgccgaacac ttgacggcaa aaatcccggc ggctgggtcc
    10801 cggaacaaaa gataaccgtt gaggaggcac tgaaggcata tacgatcaat gccgcctatg
    10861 catctttcga ggaggatttc aaagggacac ttgagcccgg aaagctggcc gatttcgttg
    10921 tgcttgaccg tgatatcacc gcgattgatc ctgtggagat ctggaatgtg aagacacagc
    10981 agacctgggt aggcgggaaa aaggtgtttg accggacgga agcacggtaa ccgcggcacg
    11041 catgataata accagacaac ttatcaggac ggcggcagtg atgatgctgt tgctgcctgt
    11101 cctgaactgt cagaacactg atgctcagat gaagagggcc agagactacg gaatagagat
    11161 tggcatactg cgtaccggca gttacaatgc cataacggat gtgcccggcg taaaggtggg
    11221 ccaggtgaca ctgaaggaag gcgactcggt aagaacagga gtcaccgcca tcctaccaca
    11281 tgaaggaaat atcttccagc agaaggtccc ggccggaatc ttcgtgggca acgggttcgg
    11341 caagcttgcc gggtacacac aggttgaaga gctgggcaac ctcgagacgc cggtggtgct
    11401 gacaaacacg cttagtgtgc cgactgccgt tgctgcggtg attgagcata ctctgtctca
    11461 accaggcaac gaaggggtga caagtgtcaa cgcagttgtc ggcgagacca atgatgccag
    11521 gatcaatgac atcaggggaa ggcatattac tacggagcat gttctggagg cgatccggag
    11581 tgcggccggc ggtcccgttg ccgagggtaa tgtcggtgca ggcaccggga cagtttgttt
    11641 cgggttcaag ggagggatag gtacatcatc gcgtgtgctg cctgtcaccc ggggaggtta
    11701 tacagttggt gtactcgttc agactaacta cggcgggata ctgcaggtca acggcgttcc
    11761 tgtcgggcag gagctcgggc gttacagctt caggaccacg gtgaacgaat ataaggaaga
    11821 cggttcgtgc atgattgttg tgatgaccga tgcaccgctg acatcgagga acctgaagag
    11881 gcttgggtcc agggcttttg caggcatgat gaggacggga gcatccggat cgaacggcag
    11941 cggggactat atcattgcag tatccacggc tgaggagctt cgtatcccct actcttccga
    12001 atcgatgtac gagcagtggc acgagatcag gaatgatgag atggatcttc ttttccaggc
    12061 tgctgcggaa gcaaccgagg aggccataat aaactcgctc tttgccgcag agacgacaaa
    12121 ggcgaggaac ggcatgaccg tagaagcact gcccgttgaa aagacactgg aaataatgcg
    12181 taaatacaac aggctgaagg agagatgatc acccgagaaa taaacaggaa ataaattcat
    12241 aaaacagcca gataatactc gacggatgag aacagccata atcttttctg cttgccttct
    12301 catggcttca tgtgcaacca aacaaccagg taatgaaatg tttacaggtc ttgattcact
    12361 gattaacgcg gaattccagg cccaagagcc cgggggtgcg ttacttgtca tgaagggaga
    12421 cagtgccgtt tatctcagta gtttcggcct tgccgacatg gtgacagggg aaaagatcac
    12481 cgaaaacacc ctctttaatc tgggctctat atccaagacc ttcgtagcca acgggatact
    12541 gatactgcag gaggaaggga agctctcggt tgaagatccc ctgagcaaat acttccctga
    12601 gtttaagaga agggagttgg ccaaaaaggt gacggtgatg cacctgctga cccacacatc
    12661 gggactccct gacaacaggc ccgtgagcga gaatccggag tttttcctca ctgcgaagga
    12721 ctacgagaac tgggccccgg tgacgcaggc ggagaacctg gtttttgaac ctggagaaaa
    12781 gtatgagtac tccaatcccg cttttaacgg ccttgcactg attatagaga aggtcagcgg
    12841 tcagaagtgg caggatttca ttgctgaaag gattttcaag cctgcaggca tggccaccag
    12901 tacaattact gacggtccgc acccggagac aggtgtttct catgcctacg agatgaccgg
    12961 cggcaaatgg gctgaatctg actacggtga gtttcccacc ttcccggcag ccggcaacgg
    13021 aggagtctgg agttcggtca gggagcttgc cctttacgag aaggccataa gggaaaatcg
    13081 tttcctcggg gcggagctta ccggcaaatc aaggacaatc tgccggcctg atacatgggc
    13141 aggtaacgag tcacccttca ttggttacag ctggttcatc ggcgaggagc agatcctcgg
    13201 gcaggccaac gtattcgggg tgaagttcgt gtatcacacc ggagaccagg gtgggttcag
    13261 ggcgtttcat ttctcggttc cggagaagga tctgctgtat gttggcctgt tcaaccggcc
    13321 gcctgaggat atggaccgga tcataatcaa gggactctct atccttaaag aaaataactg
    13381 gctcgataat taacaggtga tgcatatcat cacaggcttt taacttttta ttgctccggg
    13441 tgttatattt gccgaccgaa aaggaaataa gaaatccatg acaattcaga ttcttctgct
    13501 tctcgcaggt ctggctttgc tgatcatcag tgctgagggg ctggtaggtg gcgccacttc
    13561 actggcaaga aggtttaata tctcagatct tacaatcgga ctgacagttg tagcgttcgg
    13621 cacatcagct cccgagctgg tggtcaacct gattgcatcg tcgtctgacc atcaggacat
    13681 tgtattcggt aacatactcg gaagcaataa cttcaacctc tacatcattc tcggcatcgc
    13741 agggcttatt ttacccctga aggttcatat aagcacagtc tggagggaga tacccatgtc
    13801 tctggtggcg cttgttatct ttttctttat ggtaaatatt ccttcagccg gaggtgggcg
    13861 gatgcttgga aggactgacg ggctgattct gctcgcactg tttgtctctt tccaatacta
    13921 catatttcac cagataaaga atctcgaacg ggagccttcc gaacacaaga atcccgggcc
    13981 tctgtacagg accattggat tcatcgttct tgggcttgcc ggtcttgtta tcggaggcag
    14041 gcttgtagtc acaaatgcag tcttcattgc aaaggcgatg ggggtcagtg agaaggtcat
    14101 cggtctgaca atcattgcgg ccggcacctc tctcccggag cttgccacct ctttagttgc
    14161 cgctttcagg aagaatccgg acatagctgt aggcaatgtc gtcggatcaa atatcttcaa
    14221 catactgctg attggcgggc tggtctccgt agtcaggccg gtggcgtaca atcctgtttt
    14281 caatacggat ttttatatcc ttgccgcggg cactgttctg ctgtttatca cgatgttcac
    14341 ttcacagcgt cacaagcttg acaggtggga ggccgggcta ctcctggctg tgtatgtcgg
    14401 gtacaccacc tggctgataa tgaaagaggt ttaatacttt cttcaatcag tttttcacaa
    14461 ctgaggcggg tatgcgccca cccagggtgt gcttttcttc ttcagcctga aaaccgacat
    14521 cacctcctgt ggaaaaggtg ccggcctggc atttaggtaa aatgacggta gtggcctgaa
    14581 gtttttcagt tccgggctca tgaaagtgct gtaatcctta atcaggatgc ttttcgtctc
    14641 gaacccgggg agtattttgt taatctcttc cggagagttc atttccgcaa tacactgttc
    14701 gttctgcacc gggctccaga caatgagggg catctccttg cgttccccgg tcctcacgta
    14761 cacgttgttg tcaatggtgc tgaactgcgg atcgcgaagg cggccgcaca gcattgcgcg
    14821 ctgccagacg aagagatgag gcctgttcag ggttgcatcg gccaccatca ggttatttac
    14881 aaaaacatga ccgtcacgtt catcgacccc cgggcctgtt gccgggtgcc acccaaagtg
    14941 gtcacccacg gcgctgcgtt cgctccggct tatgcatgcc gtgctgttga cgagggtgtt
    15001 gttatacatt acagcgtcac agctgttgag gatatgtatt ccgagttcac agtttacaaa
    15061 gacgttcccg gcgcagacgg cccctttgga tatctcgaag aagaaaccgc tctcgctcgg
    15121 ccaggtctgg aggtatgata ccgcccttcc ggtcagcccg acgttttcaa cccagttgtt
    15181 ggtaaagacc gcatccacat tgccgacatc ataccagatc ccgtttgaga agggaaggtc
    15241 ggtcaccagg ttgtcattgc aggtaacccg gtagcactgg ttgaatatct tcacggctgc
    15301 cggatagtat ccgctgatgc gttcgatgtt gttccgtgtg aagatgtttt tttccagcag
    15361 gacatcggat gaactgagta cataaattcc ctcggtgctg gtgtcactca ccctgcaatt
    15421 gcgtattgta agtctgtctc cccgcaggta tgctgcaacg cgtgagcaga atgaaatggt
    15481 gcagtgttcc agggtagtgc caatcacatc ctttccgtgt tcagcttcag gtgatattcc
    15541 ttccgggtct ttcccgtcga tctcaattgc cctgtatgcg tattgggtaa aggtgatacc
    15601 tcttatcacc gggccgcgtc cgtcggaagg tctcccgttg tattcgcccg tgatgcgatg
    15661 gatgccgatg ttataagcgg ttatctcaac cagcttgtct ttcgggtcaa cgccgatata
    15721 aaccaggcct gtggaataat ctatgaagaa agtgttctca ttcacttcgc cctcgtaacc
    15781 ggcagactgc aggaaccgtc cgtcaatgaa taccatgtca tcattgaacc tgtgaagggg
    15841 agtctccttc ccgctcctca gtctctgcca ccagtcggcc ggagctgaag ggaagagacg
    15901 gtcccacttt gtgacccaca gtccgttgcc aaggtcattc cattgtgtag ccacacctgt
    15961 tcccttaagt acaggaagtt catcgccgta aggctggata gtgataccct ggttcagcac
    16021 caggtcacct gtcctgtatg tgccgcctcg catgatgatg acatctccgg tggttgcttt
    16081 tgcaatggca gcctcaattg ttgagggtgc tgcggcgctt ataccggatg cgccggggtt
    16141 gccgtcggga gcgacgtaga acgtgttgcc ggctttctca ggcaccggcc agacctgcct
    16201 taccggtccg tatggtccgc ctgaaggttg tgagcaaaca ggagccacga aggcaattat
    16261 caatgcaacg gttgctgccg tccggaaaaa aggatttaac tttaatgtgg gttctgtgtt
    16321 catattatat ggagttataa tcatgaaacc gatttcataa agttatgaat ctttaaagta
    16381 tcagttaata caatgaagac taaaagtatc attccaggag tcataatttt actggcatca
    16441 atatctgccg gaggacagga cctacaggtc aaacagtctc tggcagagat tgaatggaac
    16501 attaagaata ttaccgcggg ggtaattctg aagtcggccc gggcaaggct gttcgactct
    16561 gaccaggcga tatatgtcgt tgaaacagat accactgccg ggcggtttga gataggggtg
    16621 gctgccgggg acaaggctat cgtcaccagt gcatatgcgg ccggcgaggg agtattggcg
    16681 gccatcaacg gcaccttttt caacatggaa aaaggttata atgttcacta cgtcaaggta
    16741 gacgataccg tggtttcggt gaccgatgag aacgagtacg gaataagggc taccggggtt
    16801 ttcactgcca ccggcgaggc agttgatatc gcgagctggg ggccggagcg tgaggatgcc
    16861 ggatatctga acgctgagga tgccattgtc acaggtccgc ttcttaccga taacggaaag
    16921 gatattgcca ttgacagcac caatttcaac aagaaccgtc atccgcggtc gctcctgggc
    16981 atcaccggag agggccatgt acttttcatt gccgttgacg gcaggcagtc tggctatgcc
    17041 gacggtatga gcctcttcga gctgagacaa ctggcgagga gcctgggatg caccgaaatc
    17101 ctcaatcttg acggcggcgg gtcaacaact ctgtacgtta agggcgaggg ggtcagcggc
    17161 gtggtcaaca aaccatccgg aagagctgag agaccagtcc cgagcattgt tttcttaaaa
    17221 aagattgaat aaccagaaaa atgattaaaa atgagttata cactgaaacc tggtgaaaaa
    17281 gccatagact tccggcttcc cgccactgac ggcagggaat acagcctggc tgacttcgat
    17341 gatcacccgt accttgtggt cttctttaca tgcaatcact gtccatatgt gatcgggtcg
    17401 gacgaagtta caagggccac agcggaaaag ttccgtgaca gaggggtggc tttcgttggc
    17461 atcaactcca acagtgaaca cacatatgag gaggacagct tcccaaacat ggtgaaaagg
    17521 atgaaggagc acaatttccc gtggaaatac ctgtatgaca gggatcagca ggcagcgaag
    17581 gattacggtg cactgaagac accgcacttc tttgtattca accgtgacag ggtgctggcc
    17641 tataccggca gaggcgttga ctctccgaga gacaccacgc ggatgaccgt caatgacctc
    17701 gacagggcgc tggaggaact tacaacgggc aggccgatca gcgttgcagt caccaatccc
    17761 atcgggtgca acatcaagtg ggaagggcgt gaagccaagt ggatgcctcc cgaagcctgt
    17821 gacctggtct gagtattgtt gccgccacct gagcctgtaa cacgatatga gcacacccgc
    17881 catacaaatc tcggatctta ccaagagata cggcaaagac cggggcattg agaacataaa
    17941 ccttcaggtg gaggagggtg agatctttgg gttcatcggt cctaacggcg ccggcaaatc
    18001 gactaccata cgggtgctgc tgaaccttct gttcccgacg ggcggaagcg cacgtatcat
    18061 gggactcgac atattacggg attcgaagaa gataagggcg cagacaggct acatcccctc
    18121 tgaagtcaac ccctggcctg acatgactgc cagggaattt cttcgttaca gtgcctcatt
    18181 ctacggtatc gcgcatgacg cgaagaaaat ccctgaactg atggagatgt ttgatgttga
    18241 acccgggaga aaaatatcag acctctcatc gggtaacagg aagaagctct ccattatcca
    18301 gagttttctt cacactcccg cgttgctcat tgtcgatgag cctaccacag ggcttgaccc
    18361 gctgatgcaa tcgaggtttt tcgatattct tcgttctgag aacaggagag ggatgaccgt
    18421 ttttttttcc tcccactcgc tcagcgatgt gcagatgctc tgcgggcggg tggccattat
    18481 aagggaaggc aggatcgtca atgtggaagc aatcgagacc ctgaggaaga aacagctgaa
    18541 aaagatcagg attgaattcg tagacaggat acccgaaatt ctgaccggca tgccgggaat
    18601 gaaaaatggt gtcaatgtct cgggtaaagc catggaattt atctggtcag gcgatatgaa
    18661 cggcctgatt gctgctctaa gcggaaagga aattgctgac atgactgtag aggagccttc
    18721 gcttgaggaa atctttatgc actactacaa ttagtccgcg atgatcagat gaacaggaat
    18781 ctgtttctca gggagtttcg cagcaatgca acaggactgc tgatatggac ggcaataatc
    18841 gccttcctca ccactgccac catgtctcta tacagcatct tcctcgaaaa caacacgaag
    18901 atactggcca tgatcagcat cctccccgaa ggcgcattgc agttcaaggg tgtttccaat
    18961 gtaaacgatc tcttttccgt ccttggtttc tattccgcca ataatgtgat ttacatgctg
    19021 gtactgggga gcatctactc aattgtactc tcttccaaca tcctcctgaa ggaggagtat
    19081 ggcagaaccg cagagttcct gctttcatgg ccggtgacaa gggggcaggt cttcttcagc
    19141 aaactggccg tggttgtcat caacattttg gcaatcaatc tgataataac cgttgccggg
    19201 tacatatcac ttgagacctg taagaaggaa ccctatagtg ttgatgcgtt ttttattatg
    19261 tcagtataca ccctcctgct tcactttttt ttcgcaacgc ttggtctctt catgtcagtg
    19321 cttgtaaaaa agccaaagcc gataaccaca ctgggtgtag cgtgggtcat ggttctctat
    19381 ttcatcttca ccatttccaa tatcacacct gatctttcat gggcaggata tgtcagtccc
    19441 tacaaattca ttgatcttga tgtggttaac cccgcatacg cgttggacct ggtaaatatt
    19501 atgtacttca ccctgttttc gtttttactg atcttctttt ccctgaggat atatctcagg
    19561 aaggatattt atctttagaa agagttttca gtccggaaga aaaactaaat tgttcgggcg
    19621 gaggatcaat gaatcagtca attatggcaa tcacgaaaat cacacatata cttatggcga
    19681 ttacacttct ggtatccggc cctctgtggt cacagcaata ccagggaagc acttttgacg
    19741 aaagcggcga cgtcggaagt tgtgggatca aaggatcgac tgaatttctg ccggagagtc
    19801 aggaatatct tctgaccggg tcaggcggca acatctggtt tagctctgac cagtttcatt
    19861 ttacatggaa gaagatggag gacgatttca tcctcagtgc cgatgtgagg ttcgtcggtt
    19921 cagggaagca tgagcacaga aaaaccggat ggatgatcag ggaagggctt ggcggtgcct
    19981 cgccccatat cagtgcggca gtgcacggcg acgggctggc ctcgctgcaa tacagagttg
    20041 cagagggtgg agagacatac gagagaaggt ctccggttgt tgcccctgat attatccgga
    20101 ttgaaaggga aggtgacctg ttcatcttct ccgctgcagt cagggggagg ccatttgaca
    20161 ccgtggctgt aactcaggca tcgctggccg gcgaggtgta tgccggcctg tttgtctgct
    20221 ctcacgataa caccgttacg gaaagtgctg tattttcaaa tgtacggatt acaagaccgg
    20281 catggccagg cctagtacct taccgcgatt ttctggggag caatcttgag atcctggata
    20341 ttgagacagg ccttcgcagg gtggtcttcc aggcaccaaa ttcgatacag gcgcccaact
    20401 ggacgcctga cggcaggtac ctgatataca atagtgaagg tttgctctac cgctttgacc
    20461 ttgaatcagg caggccggag gtgatcaatt caggatttgc gaccagcaac aacaatgacc
    20521 atgtgctgtc atttgacgga aagatgctgg gcatcagcca ccatgcagca gaagaggaca
    20581 acaaatcgat tatatacatc atgcctgctg aggggggcac tccggaacgg atcaccgcga
    20641 agggtccctc ctatcttcac ggctggtcgc ctgacggacg ctggctgacc tataccgcag
    20701 agcgaaacgg tgactatgac atttataaaa tctctgtgaa gaagagaaaa gagatcaggc
    20761 tcaccgatac ctccggcctg gatgacgggt ctgagtactc acctgacggc aagtatatct
    20821 atttcaattc agcccgcacc ggtaccatga agctctggcg gatgaaacct gacggttcga
    20881 agcaggagca gcttacattt gatgaataca acgactggtt tccgcacatc tcaccggacg
    20941 ggaagcagat tgtcttcctc tcattcatgc cggaagtgaa agccgatgat cacccgtttt
    21001 acaaacacgt gtatatcagg aaaatattga ttccgggagc tgacgccggc cggcagaagt
    21061 ttgcgcccct gacacctgag gtaattgttt atctttacgg cggacaggga actatcaatg
    21121 tccccagctg gtcccctgac ggaaaaaaga tagcatttgt aagcaactcc ggtccgaccg
    21181 aatgaccgta tgataagaat aagaaagata accaatccct ttcacgaggc caataccagg
    21241 aagattgagg cagtgaagga gatactgcgc aaacagtttc cggcaataag cgaaaaaaag
    21301 atcggtgagc tggagaccca gttcgtcaat cctctcagca agaagtatca gacagcggtt
    21361 ttcgtcgcag aggatattca tgaggcagtc agggcgtttg ccgtacttct cttcatgcct
    21421 gacctgcagt tctgttatct cgattacatt gcagcatcac cagacagctc atcgtccggc
    21481 ctggggagtg cactctatga acggataagg gaggaggcca tatcgcttga tgcaattggt
    21541 ctcttcttcg aatgccttcc ggatgatccg gttttatgcc atgatccgga gacgctggag
    21601 cagaaccgga aaagactggc tttctatgag cggttcggag ccaggcctgt cgccggcaca
    21661 ctctatgaaa ctcctgtcaa acccgaagac aattgtcctc cctacctggt ctttgacggg
    21721 cttggccatc gcactgcagt ctcacgtgat gagcttcgcg gaattgtaag ggcaatcctt
    21781 gaaagaaaat atggcgatta ttccggtgag gaatataaca acatggttat taacagcata
    21841 accgatgatc cggtggctat caggccgccg cgttatacac gcaggaagat tctgcctgag
    21901 ccgacagccg ccataccgga aaagaaaaag atatggcttg cagtcaacga caggcacaca
    21961 attcatcatg tcagggaacg gggctatgtg gaatcgcccg taagggtaaa gagcattctt
    22021 aaggagctgg ataaaagcgg gttgttcagg gcaggcaggg tgcgtgagta ccctgacagg
    22081 cacatacttg atgtgcatga caggaattac ttcaactatt tcaggaaggt aagccggaac
    22141 atacccaggg ggcagtcggt ctacccctat gtatttccca tcaggtatgc ttcgagagcg
    22201 ccaaaagatc tctcggtcag ggccggttat tactgttttg acacctttac gcccctgaac
    22261 caggatgcat tcctggcagc ccgctggggt gtgaactgtg taatgagcgc agctgacgaa
    22321 ctgttggcag gaacacggta tgcctatgtc cttaccaggc cgccgggcca ccatactgag
    22381 aggtctgtct tcggcgggtt ctgttacttc aacaactgcg cgatagcagc ccattacctt
    22441 agtcgcagcg gaagggtggc catcctcgac atagactatc atcacggcaa cgggcagcag
    22501 cagatcttct acggcagaag cgatgtgctc accctttcca ttcacgggca tccctccttt
    22561 gcgtatccct atttctccgg atttgaagag gagaagggcg aagggaaagg gctgggattc
    22621 aatcacaact tcccccttcc ggagaaagtg acacatgaac agtacgtgaa ggtgctgagc
    22681 cgggcactta acctgatcaa gaaatttaag actgattacc tgatagttgc gcttggtctt
    22741 gatcccgcca aaggtgatcc gacagggaca tggagtttta cacacaacaa cttcacggca
    22801 aacggggaga tgataggcag catgaagctg ccgacgcttg tggttcagga ggggggttac
    22861 cggaaccagt cgcttggcat aaatgcccgt gccttcttca ccggactgca cagggcacat
    22921 aacggtaaaa acccaatgaa atgaagaaat cgatatttac aatgaccgga ataaagcgtt
    22981 acctgctggt tgcgggaata ctgctggtgg ccgtgatact gcttgcaaac aagctggtaa
    23041 tatggggcgc cgcattcatt gccgccgggc tgattatcct cgggatacag cagttgatcc
    23101 agctgaacgt gaaggtctca aagttcgagg ataacatctc cggccttcag gaaaagaatc
    23161 aggatctggc gaagatgaat gagaagctga tagaggaaaa tgcgttcctc aaggagcggc
    23221 atttccagat cacgcagata aagagcatcc tcgagatgaa tctcttcgag attgacacca
    23281 ggttcaagcg gacggtcaac aggacggaga tgctgaatga ccgtgagatc aggcatatcg
    23341 gaagcctggg tgtctcgctc aaggccaagt acggcattga tcttaaggag ctcaggttca
    23401 agtatatgcc tgaaagtgat gagctgattg tggccaatat caacccgcgg tttctttcat
    23461 tcggcaatcg caagcttgag tgggagttct tcgagacgct ggagtacagg aggcagttgc
    23521 ccctgacgac gaagaggtgg atgaccagtg atgacctggc ggagaaggcc gacaggctga
    23581 aggaggaaat tcgcctggag acagagcatt cgcttgagag cgggccggaa gagttttcat
    23641 ggatcatgga acccatccgg gagaacgtag agaatactat aaggataatg ttcaggggta
    23701 tatgcagcaa tatccgcata gcagaactgg ctgacaacag tttcgtaccg ctggaaggaa
    23761 tcaggctgga aagactggaa gaacaaaaga aactcaaata gtttttcaat gaaaaggatt
    23821 tggattgcag ccctcgtggt gattgccctc gcctcaggct gcgcgacttc aggaacggtt
    23881 gacatggcca gtcctgatgg aaggatagtg gtggcgttta acctgctcga gggcgggcaa
    23941 ccggtatatt cggtcacata tcgtgatacg gctgtgatta ctgaaggcaa actcgggctt
    24001 atcaggagtg atgccggctt ttcgtcgggt ctgaggctga attcagtgac aggaccggag
    24061 gaggttaagg agaattatac cctgctgcac ggcaaaaaaa gggatgcatc ctaccatgca
    24121 ttcaggtaca ctgtccacct ttcaggtgca tccggtgagg caatggatat tattttccag
    24181 gtttcggatg aagggttggc attctgctat aaatttcccg gtgtgtctga agagattaaa
    24241 tacatcacag ctgaaaagag ctcattcggt tttgcagagg gtgcgctggc atggatccag
    24301 ccgcgtgcca aagcaaagac gggctggaat cagtcaaatc cctcttatga agagaactac
    24361 ctgatggagg tgccgctggc caacgtggct gctgacacca acggatgggt cttcccggca
    24421 ctatacagat cgggcggttg ctgggtgaac ctgacggaaa gctggcctga caggaactac
    24481 tgcggcagcc acctggcaaa aggagaagga gccaatgaaa tgatgattgc cttccctgag
    24541 cccactgaag gttatacagg cggttccgtt ttcccggaat caaagctgcc atggcagaca
    24601 ccctggagga tagtgacgat gggcagcacc cccggtgcca tagcagagtc aacccatggc
    24661 actgacctcg ctcttccgcc agctgaaggt gaattcagtt atgccaaacc cggaagggcg
    24721 gcatggagct gggccctgat gaaagacgga tcagtcaact atgaggagca gaaacgtttc
    24781 attgcctacg ctgctgagat gggatgggaa tattgcctta tagacgtcaa ctgggatcag
    24841 acaatcggct gggacaggat agaggagctg gcgaagctgg cacaggcaaa aaaggtaggc
    24901 ctgatcttgt ggtacaactc agcaggcgac tggaatacag ttacatatca cccgaaggac
    24961 aggttgctga ctcatgaatc cagggaagag gaattcagca ggctggagag tcttgggata
    25021 aaaggaatca aggtcgactt ttttggcggt gacggacagt cgatgatgga ttattaccag
    25081 gatatccttg ccgatgcata caggcataaa ctggttgtga attgtcacgg atctaccctg
    25141 ccgcgcggcc tgcaccgtac atggccgaat cttgtttcga tggaagcgat acgcggattt
    25201 gagtatgcca cctttgacca ggggacagct gacctggtag catcaaaagc cacaacactc
    25261 gcatttaccc gcaatgcctt cgatccgatg gacttcactc cggtctgttt taccgagtat
    25321 gataataata aaagggtgac aggcaacggg gccgagctgg cgctggccgt gctcttcctt
    25381 tcaggggtgc agcattatgc agagacacct caggggatgg caaccgttcc ggactatgtc
    25441 agggagatga tgaaggagat cccggtaagc tgggatgaga caatgtttat agacggtcat
    25501 cccggaagat acatcgtgct cgcgaggaga tcagggaatg tctggtacta tgcaggtgtg
    25561 aatgctcaga aggatactct cgggctaaat ctgaaactac cctttgccgg gggcatttca
    25621 ggaactatca ttaccgaggg tgagacactc cggtcattca cgcaggatac tgtcgtgcct
    25681 gacgggtcag gaatggcaac cgttcagata gtgcccggcg gaggctttgt tatcaggttt
    25741 gaaaaacctg cgttataatt tcagaaccga tgaagaaaaa catactgtac ctgttgcttt
    25801 cacttttaac agctaccggc tgtgatatgc ctgacaaatc tcccggagac aacctcctat
    25861 ggtatgataa acctgccgcg gagtggaccg aggctcttcc ggtagggaac ggacggctgg
    25921 ggggaatgct ctacggtgat ccctcggttg agcatctgca ggtgaatgag gagtcgctct
    25981 ggggaggtgt aaaggtaccc aacaacaatc caggcgcgct tgagaatctg ccacggatca
    26041 ggaaactgat ccttgatgga aatattccgg aggcttatga actcgctgaa gaacatctgt
    26101 caggtatacc gggaaaaaca tggtcatacc agaccctggg agacattttc ttcaggtttg
    26161 cagacactgc agccactgtg actgattacc gccgggaact gaatctttcg accggtattg
    26221 cttccgtcag ctttaaaaga aatgggaaaa aagttgagta tgaagtcttc gttcctgccc
    26281 acgaggatct gattgtaatc acaatgaagt cgcaggaaga gggtgggctt gatatgcaga
    26341 tcatgatgac caggccccgg gatgctgaga taacagttac tgagaacagg attgacatgg
    26401 aggggcaggt tgttgacctg gttgaccctg agagaggacc ggcaggtgag cacatgaaat
    26461 tttatgcatc actgataata acaaactgtg acggtatcct gaccccggaa gaaaacagcc
    26521 ttggctattc cggaggcaag gagatcacgc tcttcttcaa tgcccgcact gactacaata
    26581 ttgattatct caactttgac cgggcgattg acccgcgaaa gatatgcact gaggcaatca
    26641 gcaaggcatc atggacatcg atgagccgga tacgtaacgg gcataaaaca gagcactcat
    26701 ccatgttcaa cagggtgaag ctctcgctgg gcggcgatcc tgcactgaat gagctcccca
    26761 ctgatgaacg tctgaggaga tttcaggccg gtggtgatga tcccgggctg attgccacat
    26821 actttcagta cgggcgttac ctgctgatgg gatcatcacg ttatcctgcg caactgcctg
    26881 ccaatcttca gggcatatgg aacaaggata tcaatgcccc ctggaactcg gattaccaca
    26941 caaacatcaa ccttcagatg aactactggc cggctgaagt gtgtaatctg accgaaactg
    27001 caggtccgtt gatacgtttc atggagaaat taaccggtcc gggaagtgtc actgcacgtg
    27061 atacatacgg tgcgaagggg tggactttgc atcacacaac ggatgtcttc ggcaagactg
    27121 cggtgcttga cggtgtatac tgggggatgt ttcccctggg gggagcatgg atgacccttc
    27181 cgctatggga acactatgaa tttacaaatg atactgctta tctcagggag catgcttatc
    27241 ctgtcatgaa gggtgctgcg gatttcatag ggacattcct ggttcctgac agccagggcc
    27301 gtcttgtaac ggttccctcc tactcgcctg agaacagtta cattgatccc gtttcgggaa
    27361 aggagttcaa gctgacctac gctccgtcga tggacaacca gatcatccgc gaggttctcg
    27421 gcaactgccg gaaggcttct gcaatccttg gtgttgaccg ggagtgggct gactccatgc
    27481 aattcattct tgacagggtc cctcccgaca gggtaacatc gaacggtaca atcatggagt
    27541 ggattgagga gtttaaggag gccgagccgg ggcacaggca catgtcgcat ctctttgccc
    27601 tgcatcccgg ggcgcagatt accccggaga ctccggagct gtatgctgct gcgcgccgca
    27661 cgcttgagcg acgtcttgaa aacggcggag gacataccgg gtggagcagg gcatggataa
    27721 tcaacttcta cgcaagactt cttgacggcg atgaggcgta caaacatctc aggcagttgc
    27781 ttgagaaatc gactttgccg aacctgtttg ataaccaccc ccctttccag atcgatggca
    27841 atttcggcgg gaccgccggt attgccgaga tgcttctgca gagccagaac ggggtggtgc
    27901 agatactccc tgcactgcca aaggcatgga ctgaaggaga agtgtcagga ctgaaggcca
    27961 ggggcaattt cgagatcggt atagagtggc tcggcggtgt tgctgagaag atcacggtga
    28021 agtcactttc aggtgcgccc ctgaacctcg cctatcgcgg aaatattaac tccatggaaa
    28081 cggaaaaggg tattgtatac aaatttgaca gcaacctgaa atggatagag taattttctt
    28141 catcgccctc agcctgccgg ttatcttctt ctcaaagcag agcctctttg agccgcacag
    28201 ccacggattc acgaggttct tcagctggga atgcattatt attctttttg tgtttaatta
    28261 cactgcatgg tttaaggagc cattctcagc caggcagata gtatcctggc tgttgcttat
    28321 cgtctcgctg tggttcttga ttgagtcggt ggtgcggata aggcgcgcca ggaagcctgg
    28381 tgttgtcagg gtcgacgaga agctgttcag gttcgagcag accaccgaaa tggtcacctc
    28441 aggaatatac aggtacatca ggcatccgat gtattcatct ctgctgtttc ttacctgggg
    28501 catatggttc aagcagccgc tctggtatct gctgccagta gcatttctgg cttcatggct
    28561 gctgtatctc actgcccgcc gggacgagaa agagtgtcag gcgtactttg gagagaagta
    28621 cactgaatac atgaaagaga cgaagaggct catccccttc attatgtgag cccgcaggaa
    28681 gcgcggatgg ttaacagtca gagattcttg tcagggtaca tctcgttcca gtgctgaggc
    28741 cggttcctga gtttcatgtc aaaccatgac atgattgtgt tatgccattc aatacgcttt
    28801 ttatagtcaa gaatgtgatg gttttcgcca ctgacgagga ccatctccac atccttgccc
    28861 aggattttca gggctgcata gtactgcagg ctctcaccta ccggcacgtt tgtgtcagcc
    28921 gttccgtgga ggagcagtat tgagttactg aatttgtcag cgttgaataa cgggctgtta
    28981 tcgacgtaaa tatcgcgcct gttccaggga tagcttccct tggtggcacc cgaactgtag
    29041 gaataacccc agtatccttc accccagtag cttgtaatgt cactgatgcc ggcatgggca
    29101 attgcagtcc ggaagatatc agtacgggtc tgcagcatca tagtggtgaa tcctccatat
    29161 gaagcgccga cacatccgac atttgcagca tcggcagagg ggaacgctgc caggaacttc
    29221 tttgtacctt caattatgtc atcaattgcc tcgcgccccc agccgtttac gtgcagggct
    29281 gagaaatcct gcccgaatcc tgtcgatccc gagggctgca ggacataaac catatacccc
    29341 tcggcagccc atacttcctt tggataacgt cccccgaaag acctgtcggt gggagttgtc
    29401 ccgccataga agtttacaat cacggggtac tttttggccg ggtcataatt taccgggtag
    29461 tatacccttc catagatggt tattcccctg gagcttttga aattccattc ttcagtccgc
    29521 cctttcagca catatgccag ctggtctgca cccggatcag agagcaagga tgatttcccg
    29581 gttgagaggt caattgcata aagttttacc ggagtgctga tgcctgttcc gcggagtaaa
    29641 gcaacattac ttttttccgc atagtcaacg gaagtgatga cctccaccgg cacatcgatt
    29701 tttgtaaagt tcctcctttt cgggtcaagc ctgtagaggt tcacgtagtc gcgttccgtg
    29761 gcagtaagat atatcttgcc gtccggggcc cagcgtgcag tcccggctgc cgggtcaaaa
    29821 ttgcgtgtca gaggctcggc ttttctggtc ttaaggtcaa agagataaag ctgtgtatca
    29881 tagctgtttg gaatccggcc gtcgctaacc ttcaccccca ggctgccgaa agtctccggt
    29941 ccgcccgata cgagcagttg cgtaccgtca ggtgagtact ggcatgaacc gccgtacagc
    30001 ttgtcaatcc agattgtatc aaggcgcatt gtactgattt ccagttcata gagagtctgt
    30061 tttgagaaag gaacctcgct gtagtcctgt cgtgaaacgg agaagagaat ccgccggccg
    30121 tcagggctga ttgcgtgcag cgttgctgaa cttgatccgg cggtaatagg ctgtgacacc
    30181 ttcgtggcag agttatagat atacagatag ctccggttgc gagttccggg aatacggtcc
    30241 tcattgtcaa aaacccgttt cagatcacct gttttctcag cctcctcatt ccgtgagtac
    30301 acaaaataat cttctgccgg tgaccagaca atgcggccgg gcgatttcag gccggatgcg
    30361 gcaacgctct ccgtaccggt cttcatgtca tacactatca catcactcgt cccctccctg
    30421 tcaatctggt aataaaagcg gtcggatttt ggcatccagt cggcacggag ggcattgttt
    30481 cgcagagcga agaggttttt gttcagctcc atatcataaa cctgtaatga gctctggggt
    30541 ttcccgctgc ccggcaaaat ctcggttgtc tggattagaa catatttgcc tgaaggagaa
    30601 agtcttgctc ctgatatgct tactccttca aggatatcgt tgattgtaag tgtccgggcg
    30661 gggttcgtgc tccatgacag cactgccgaa tcgtttgctg ctgatacgtc agcggcgaac
    30721 ctgagtgttt tttccgtgct gatgagcttc agccctacct catgaattcc tgttcccagt
    30781 gtcaggtcaa tcttaactgg cttgtccgaa ggtttgccct gcgatttcac ctttttaccg
    30841 ttgaggctga gctcaaagac tgcattggtt gaaatattaa ccgaagcttt ggtccaccgg
    30901 tcgctgctga tgaaaccggc catcaggaca agctggttgt ccctgttgac tgaagtgaga
    30961 aggctgtcgt ctggcagggt tacaacgggc catgatttta ggtctgcatc atcagcctgt
    31021 acattctcga gaagggctgc cgtatcaaat tttttcccgt caaccgatgg ctgatcagcg
    31081 aagaacggca gaaagagctg gtcaacgccg gagattctcc agtcattaca tacgattttg
    31141 ttttgtgctg ttgatgctac tgcaatcagc ataagtaata aaaggccggt gagttttttc
    31201 attctatttg atttttattg atttcggttg tgtccgggag aaatccaaat aacctatctc
    31261 caggtgtcaa tcctgtcaag cggatagatc ggtcttggga tatgcacata gtttagtgcg
    31321 aacggatcag tgttgctcag tccgggtgat atcacattga cagtcttcct gttgtaaagg
    31381 aggtagttgt acctgaacgg gaataggttc ttcacaacca cgatatcggc tttccagatt
    31441 gagagcccaa ggtctttgaa gtcggccggc gttcttgatg ccatcggcag ttcagagata
    31501 ataaggtgta tgccgttttg tttcactatg agtgtctttc cgaatgaagt ctcctgcttg
    31561 agggtcacaa caccgttgta tgtgaagggc tggttataga cggtgtctat cttccctccc
    31621 actgtcagtg tcacctcatc gccgatattg ctgttccatg cttcgagtgc ggcagcctca
    31681 tcgcgcaggg cgatatatga ggtcagctca ctgcccttct ccacaagggc tttcagtatc
    31741 caggtgcttt ctccgggggt tccggtacct acggcatcgg agacatcgca gaagacaagt
    31801 gtcccaagtg cccgtgcaac cttcttctgt tgtgcaattg cgaccgcctc tccggggctg
    31861 tttccttcag gctgcggcac atcccgcact gcccaggcca tctccgagat ttcatcggcc
    31921 agttcccgtg acaggtcagc cttgtcgttt gtgattgcga ttgtactgta acccagctca
    31981 ggatcatcta tccagatatg gaccgggaag aatgagacgg aaagaacatc ttcctgtttc
    32041 tccatctttt tcattgcaga aaatatcttg ttgaacgggg gaaggaaatc gacattgatg
    32101 cctcctccct tgaggagctt catcttgttt accaccataa ccggttcaat ttcacccata
    32161 acagttctta tcaggagatc acctgcctta aaggctgtct tgaaatggtc acggtgggga
    32221 tttgtatggt atccgacaat tatgtctgcg ttctcagccc tgcgccgggt gacattggca
    32281 tggaggtcaa aggttaccgc aattttcaca tccgtgccaa gcgtatccct taatgaactg
    32341 atcaggtcac cttccgggtc aaacatcccc tgcactccca ttgcgccgtg aagcgagagg
    32401 tagataccat caaccttctc ctgcccggta accgcatcaa tgatgaggtt ttttattttc
    32461 ctgtagaaaa ggctgtctac cggtccgcca gacattgatt tagcctggag tacgggtatt
    32521 gccctgatcc tgcctctgcc gcttttctca actgcggaaa ggaagcctgc cagttgtttg
    32581 tcctctttaa ctgccgaagc ggagacatcc tcgccgtaaa aaaggtggtc agatttgaag
    32641 ttgacttctt tagttattac aggagagaaa ctgttgactt catgcatgaa ttcagcatat
    32701 agaattacct tctggtctga ggggctcccg gatgaatttt ctccggagca gcccgataag
    32761 gcagcgactg tcaccgaaac cagtacataa atgattttac tgaaacggac ttcaaatttg
    32821 gctttcatga atattggtat attggacaaa aataggaaaa acaggattta ccttgcctgt
    32881 gcgggtaatg ttaaacaata tatgtttaaa atggttttaa gcatcaaaag ggaacactaa
    32941 tgaaggcaga aaagaggatt gttgttacaa tactgatgct aatgactgtt gttgtggtgg
    33001 tggctcagga cggtagccgt gcattcggag aagtcagcct tgctgccaat aatgcaggga
    33061 tgtatgttct gggaggctgg gcactggcaa atatggcggc gggtgcttac ggatgggctg
    33121 catttgaggg agaaaaaaag tatttcagtc agatgaacct gttctggaat attgtcaacc
    33181 tgtcaattgc aggatttgct ctgtacagca actacagcac tgacctgcta tcccttggcg
    33241 atggtgagat catgacgaag catctcaaaa cggaaaggct ttttctgata aactcaggtc
    33301 ttgatgtggc atatatgggt gcggggattc tgatgaggca cctcggtact ggttcggaga
    33361 ggagaggtga tctgcttaaa ggctacggca acgcagtgat actacagggc ggtttcctcc
    33421 tggtgttcga cattgtgatg tatttcatct ttcgtgatat aagacccgcc gttatcgaat
    33481 taacctcagc gttgcagcag ggaactctgc attctggtat cagaattgca ttctgaccag
    33541 ccggatcccg ggaatcagca tgacttccac aatgagtatc cctgttagtt ggtgtagtgg
    33601 cagtgtcctg gagcacagcc aggtaacggt ttattattca aaagtcattc cggttcggga
    33661 agagggcggg agcgcagcca gttaaccgct gagtctgaag ccagggtttg gttgaaaagt
    33721 gctccctgtt ccttctcccc tttaaggaag caatccagcc acgggaccat ataccttgca
    33781 agaatggcgg actgctgttc agtattcagt ccctgcctgc agccagcaag tctctcggca
    33841 gtgaagcatt tgcggctgtc gcccatgccg caatgtgaac cattgattat cgagatataa
    33901 gtcttgtttt ccgcacccga ccgctcatac atcggctggt gattcttttc aggagaggtt
    33961 atgcaatcgc tcgtgccgga aaaaatcagt gtagggacca ttacgaggga agcagcttcg
    34021 atggcggaag gcctgatctg ataaggtgta agtgcaatca cggcatcagc atcagcgttg
    34081 ccggcggcgg cgtgaaacat ggcgcctcca cccatgctgt gccccataag gcagcatatg
    34141 gtatctgcaa tcccgtataa aggtgaagca ggatcattgc ccagtccggc cactgatgcc
    34201 gcaagaaacc tgacctcatc ggcgtagcct atgtgagacg gcagcgggcc ttcaaaagtt
    34261 gtcaggctga gcattacata accagccggc accagaatat tcaccaggtt gccgtactgg
    34321 tctcccgggt gctggtatcc gtgagcaaag cagataaccg gaaacttgca gcctgtcatt
    34381 ccggagggcg cagcgctccc cgcctgagcg gtcacagggt agtaagcctc gaatttcacc
    34441 ctcctgtttc cccgggattc atccctgagt tccatcctgg catgaccaat agtatactgc
    34501 gctgatgcgg ccatgcaagt gagcaaagct gtcataagcg ctgcagttct gggtgaaaac
    34561 atattcagcc tccggcatag tatgaaaccc tgcacccctg gtggagggaa gcgccaaatt
    34621 actcaatctt tccttcagcg gcaacgcctg gagagcgcgg atatcatggt tttaaaatga
    34681 ttattatttt tacactgcgc tgcaggagga tggacataaa acagccggac ctggcagcgg
    34741 ggaatattta tgctatggaa aagactctct tcgatctcct cgtctggctg tggatctcag
    34801 ttgccgtggt catttttatt gttctgcttt ttatcacggc gccgttcggc cggcactcga
    34861 agaacacatg gggtgtgaca attcctgacc gcatggggtg gttcctgatg gagatccccg
    34921 gaccggtcat ttacctgtca ctggtcatcg caggcattgg acccaaaacg ttgacagtat
    34981 ggattataac aggcctctac ctgttacact acaccaacag ggccatagta tatcctctga
    35041 gaatcagaac ccgtggaaag cagatgccac tggtggttgc aataatggca gtccttttca
    35101 attttgtcaa cgccggcttc cttggatact ttgcaggctc cctccagacc cactacactg
    35161 atgcctggct taccgatccg cggttcctga cagggcttct gatattcatt gcgggaatgg
    35221 cgataaatat gacctccgac aacaggctta tccacctcag aaaaaagagc ggcgacgggt
    35281 accagatccc ccggggcggt ttgtttgaga ggatctcatg tcccaacctg tatggcgaga
    35341 ttgtggagtg gggagggtat gccctactgt gctggtcgct gccggcactt tcattctttc
    35401 tctggacctt cagcaacctg gtgccgaggg cattgtctca tcacaggtgg tacaggagcc
    35461 atttctccga ttacccgaca ggaaggaaag cagttctccc ttatattttg taatttgccg
    35521 tatcaaaccg gaatgccatg aaaggatcat atttaaactg gaaaggacgt tttctctgga
    35581 tagcaatctt tgccatttct atgggcttcc tggaagcaat tgtagtagtc tatctcagga
    35641 aactgtacta tcctgacgga ttctcttttc cactgcgtct catgtcggcc gacctggtga
    35701 ccgctgaatg gatcagggag attaccaccc tggtaatgct ggccggcata ggcatcattg
    35761 ccggccgcaa cggtttgcag aggctttttt atgccctgtt tgcatttggt atttgggata
    35821 tagtctatta tgcagcgctc aggatttttc tgggctggcc tgagacactc atgacatggg
    35881 atctcctctt cctgatacct gtttcatggc tggggccggt gctggcgccg gtgatcaatt
    35941 cactgacaat gatcatgatg gcgctgcttt tcatagggag acaggaaaaa ggattttaca
    36001 tcaggccggg tctctcagac tgggtactga tagtcagtgg cgcggtggta attctctata
    36061 cttacctcgt tgactattcg cgcctgctgt ttgagagcgg ggccctcagc gcaaaggagt
    36121 cgccggaggc aggagaacgg ctggctgtga tgataacaac ttatgttcct gatcattaca
    36181 aatggactct ctttattatc ggggaagcac tcatcatagc tgcaacaata aacgtattaa
    36241 taagaagtcg caaatattca aaagatgaaa cagttaacta acctgacggc attgattatg
    36301 ctattactga ttttacccgg ctgcggagat aaaaagagca aaagtgcaga ggccactgac
    36361 ccctggctgt ggcttgagga agttgatgcg gaaaagtccc ttgactgggc ccgtgaacag
    36421 aacagactct ctgatgaggt tctgacggtg agaccggaat tcacggagct gcgtgagagg
    36481 ttcctcgggg tgctgaatga cagggacagg attatctacc ccggcattac aggagagatg
    36541 agctataacc tctggcagga cgaaaagaat gagagaggtc tgtggagaag gatgtctaag
    36601 caggattacc ttgcaggaaa ggatgtgtgg gagacggtgc tcgatctgga cctgctttca
    36661 gcggaagaag ggaaaaagtg ggtcttcggc ggagcctcat ggctcgggcc ggaaaaccgt
    36721 tactgtctgc tctggctctc tgacggcgga aaggatgaga gttacatccg tgaatttgat
    36781 gctgtggcaa agaagtttgt cgggggagga ttctccatac cggaaagcaa gggaagcgca
    36841 gcctgggtcg acagggacca tatcctggtt gccaccaact atggtccggg tacgatgacc
    36901 tcgtcgggtt acccggcagt agccaaactg tggaagagag gcgaggatcc ggcaaatgca
    36961 gtcatcatgc acaggactga taccgccaac atgggtattt ttgtcaacgg cggagaggtt
    37021 aacggaaagc agtatgtttt tctgaatgag aatgtcggtt tctatgagtc gtcggcttat
    37081 cttcttaccc ctgagggtat gaaggcactt gacatcccga aggatgttga gacaagggga
    37141 atgttcaggg aggcgatgct gctatacctg cagtctgact ggactctcag cggaatgaca
    37201 tataagaagg gtacgctggt cagttttgat atcccatcgt tcctctcagg tacggctgtg
    37261 gtaaaaacac tttacgagcc cggggagagg gagagtcttg ccggcgtaat gtcaacccgt
    37321 gatttcatcg tgatagcaac gatggaaaac gtccagggaa agctgtggat catgcgtcct
    37381 tccggtgaca gctgggtcac agaggcagtc ccggtgccgg aattcggcac tgtaggcctt
    37441 gccggttcgg atgacaggtc aaatgactac ttcttcgcct tcagcaatcc ggtcactccc
    37501 tcaaccctgt attatggaga cgggaagctg atcaggccga tcaggaagca gaaggattat
    37561 ttcaatgcca caggtgtgaa agttgaccag tatgaagtag catcaaagga tgggacgaag
    37621 attccgtatt tcattgtata cccggcagag atggcccctg actgcagcaa tccgaccctt
    37681 gtttatgcct acggcggttt caactccgcc acccgtccgg gctacagttc catcaccggt
    37741 atcggctgga ttgaaaaggg tggtgtttat gtcattgcca atatacgtgg cggaggtgag
    37801 tttggccctg aatggcacca ttcggctctg aaggagaaga ggcagaatgc ctatgatgat
    37861 ctgtttgccg tggcagagga tcttatctcg agaaagatca cctcggcaga gaggatgggt
    37921 ttctacggct ggagcaacgg cggattgctg gcaggcgtgg ccatgacgca gcggcccgat
    37981 ctctggaatg cggttgtcat cggggctccg ctgcttgaca tgaagcgtta taacaagctg
    38041 ctagccggcg caagctggat ggacgaatac ggcaatcctg acgtgccggc tgactgggag
    38101 ttcatcagca aatactctcc ataccagaat cttaagacgg atgcaaagta tcccactccg
    38161 cttttcgtca cctccacgag ggatgaccgg gtacatccgg gacatgcgcg caagatggcg
    38221 gcaaaaatga ttgaaatggg acatcctgtt ctttatcatg agaccataga gggtggtcac
    38281 ggtgcagcat cgaccaacgc ccaggaggct gagatggaag cgctgatctt tacctatctt
    38341 aatatgaagc tcggcaaata gtcgtcaccg ggcatcctga tcatcagcct gcataaaata
    38401 tggaaggctg ccggttgggg ctccggcagc cttcgctgca ttaaaacgga cttatggaaa
    38461 gaaagaaaga atcagataaa tcaggtacct gaaactcttc aatccggtac cataacagct
    38521 aaccactttt gaaatgtact ttatccatta tgaagatttg atgaagaaaa cgtggccggt
    38581 caatccggat catcattttt taatgatgat aattcagttg aatcgatgat atttcatatg
    38641 atacaatttg ccggtataaa atttttatat atttatagag cctgatttta gattttgtaa
    38701 cctaaccaat accaccatga ccagaacaaa acatgtattg aatgcgcttc tttgcctgat
    38761 gatcattctt gcgtcctgcg gcggcgggaa gggcacggag gcgaagaaag cgcctgaggc
    38821 agtggcggaa acagcaactg taagcctgtc gatagaaggg atgacatgca ccgggtgtga
    38881 aaacaccata tgcaccagcc tggaaaaagt tcccggggtg aagtcggtca ccgcctcaca
    38941 tactgacggc aaggcagtca ttgaattcga accgggaaaa gctgacacgg caatgcttaa
    39001 ggaggcagtg aatggcgccg gatataaagc actgaagatc agcgcagttg ccgacggaga
    39061 gaaggaataa gattccattg cagacaaatc tcttaaacat caagctgatt accaatgtcc
    39121 agagacaaga aggaagaggg taatcgcagc aatgaccttg acagggtaat tgaaaactcc
    39181 aggcgtgatt ttctcaggaa gctcacgctg gcgggagcgg ttaccgtcac gggaggagca
    39241 gcgatttact caggccatcg cttcgagaag gccaagcacc gggaaggtta cgtcaaggta
    39301 ctcaccgaag acaaccgcct tgtggagata cctgttgaac agatgcgcga tttcaagcct
    39361 gacctcagga tgcttcagag ccgtggcagg gagggcatag ccgggaagag gtgggtgatg
    39421 gtaattgacc tttcaaagtg ccgcaatgcc agaaagtgcg ttgaggcatg ccagagcgcc
    39481 catcacctga gaccatatga atatcatata aacactcttg tgatgcagga gtctgtaaac
    39541 actcctgcat tttttatgcc caagccgtgc cagcactgcg acaatccgcc gtgtgtgtcg
    39601 gtatgcccgg tggatgccac cttcaagagg caggacggca ttgtactcat tgacaacgat
    39661 aggtgcatcg gctgccggtt ctgcatggca gcatgcccct attcagcaag gatattccac
    39721 tggcaggagc cttcgaaaca tgaggaagac ggcaataaag aatacgatgt tgagctgaat
    39781 gttccgcaga agaagggtac tatcagcaaa tgtctcttca gtgctgacag actgagggaa
    39841 ggaatgctcc cgtattgcgt ctccgcctgt cccaacggtg tcttctactt cggcgacgag
    39901 aacgaggatg ccgtcaccaa cggcacaacg aaggagacgg ttcgcttcag cgaactcatc
    39961 gagaagaatg gcggttacag gctgatgcct gaactgggga cccagcccag ggtttattat
    40021 ctccctccca agaacaggat ctttgagttt gagcagggag aaaaaagaga gtataagcag
    40081 gaaaaagcat aaaccggctt caccatggat gagatacgct tcaatacccc gcatgagaag
    40141 ggaaagattg attccctggc ctctgacatc ctgaaaagtg tccggatcaa tgccggtttc
    40201 ctgctgtgga tgactttcct tttcacattg ctggccgcct gtctttttgc ctatataata
    40261 cagctccgca agggactggg tgttaccggt cttcgtgatt tcacaagctg ggggatgtac
    40321 atcgccaact ttgtattttt cgtggccacc agtctttgcg gtatgctgat cagctctgtg
    40381 cttggactga taggctacaa ctgggtgaaa cccatttcgc gcatagccga gataatagcc
    40441 attgccttcg ccgcagtggc gggacttgtc ataatctccg acatgggccg tcctgacagg
    40501 cttccctatg ttttcctgtt cggcagggtg caatcgccca tactctggga tgtgactgtg
    40561 gtcaccacct acttcgtgct aagcatgctg ctatggttct ttccgatgat tcctgatctg
    40621 gccattgcga aggaccggct tgaggggaga ccgaaattcc tcatcagggc ttacgagtta
    40681 ctgtcattca ggtggatgca tcatgaagtg caatacagga tactgggcca ggcagtgagg
    40741 atacttctca tactgatcat tccgacagcc tttgcaatcc acactgttac ctcatggctg
    40801 tttgcggtga cgccacgtac cgggtgggac agcaccatct tcgggccata tttcatctca
    40861 ggggcatttg tatccggagc agcagccgtt attattgcca tgtacttctt cagggtgaac
    40921 tacaagttgg aaaagtattt gaatgccagg gtttttgaga gaatgggaag ggtaatgatc
    40981 ctggttgcaa tagtttacct ttattttaat ctgaatgagt tccttgtgcc ggcgtacaag
    41041 ctgaagacgg gtgatgccat acacctgagg gagctgttta caggccatta tgccatgctt
    41101 ttctggggca tccagctgat cgggctggtg attcccattt tattgctgct cttcaggcag
    41161 atgcggaaac ccctgccgat gatgataatc tccctgttcg tgctttgtgc ttcatggttc
    41221 aaaaggtaca tcattgtggt tccgccgcag gctcacccta accttccggt acagaatgtt
    41281 ccggttgagt ggatggttta cagccccact ctcattgaga ccgcagtaac cgtagcctca
    41341 attgtgctgg tgctgattat aatcacaatc ctctccaagc tcttcccggt gataccgata
    41401 tgggagatgg cggaagagaa agaggatgca gaaagaatta tccaataaca gaaaacgctg
    41461 agttgatatg aaatatacaa caggagtgat aatatgcctc atgctgctcc cttcgtttgc
    41521 ccgggggcag gactgggtgg ttcccgatga caggaaaggg agactcagca cattcccgtt
    41581 cacggatgat acgaggaaag agggagagaa attataccag gtcaattgtc attcatgcca
    41641 cggtgatccc ggaaggaaca acttcattgc gcttcagccg cctcccggtg accctgccac
    41701 cgaaaaaatt cagaggaaca gtgacggtga gatctttcac aaggtatccg ccggacgcgg
    41761 gcagatgcca tcattccgga gcgtgctgac caccgatgaa atatggaagg tggtctcctt
    41821 tatacgaagc ttcaaccgca gctacgttca gaaggtaatg caggtgataa cctcctctgc
    41881 atacccgggt gcaattatca gcatgaagct ggcttacgat gaagcgacaa aacaggtcct
    41941 ggtaattgcc tctgccacaa gcgaaaccgg aactgttccc gttaccggag ccggggtgcg
    42001 actgctggcc gcaaggtatt tcgggaagat ggctgtggat gaggaaaaaa caactgacgt
    42061 caacggtgtg gccaccttca gtgttccgga aggtttacgt ggagataccg ctggaaacat
    42121 aggtttcagt gccatgttca ctgacgaagc gactttcggg agtgtctcga aggacacggt
    42181 gctgatggca ggaatgccgg ttatcccggt aagcctcact gccaatagag ccatgtggaa
    42241 cactgtcagg aaggccccgg tctggattat cctggcatac ggactgggag tactggcagt
    42301 ctggggattc atcgttctcg tgatgttcaa actccgcgac atctttactg tgggcactgc
    42361 tctcagtgag aacatttccg cttcacacgg acaaacagaa ccaaccaaaa actgacagat
    42421 atgaagaacc tggccccaat cggacgtatc ctgttcgcgc tgccatttgc tgtgttcggg
    42481 ataaaccatc tccttatgat ggattactac ctgggtatgc tcacctcgtt cattccgctt
    42541 ggcggatata caataattct tacgggactg gtgatgatta tcgtaagcat cagcatcatc
    42601 acaaagaaat ttatcaggct ttccacactg atacttgcag ggctgctgct gatattcatt
    42661 cttacaattc acttgcctca tctgtttgac ggcactgaca gggttcccat aatgattgcc
    42721 ctgctaaagg atatttcgct tatgggaggc tcccttatga ttgcaggata ctgcgctgaa
    42781 gaagaaacaa aaaaagctga aaaatgaaga tatttaaagt tgccgccctg gcatttttta
    42841 tttttctctt ttcgccggca ggcgcacagg agataaaaac agcagggtat aaacaacccg
    42901 aaccggaagt aggtgttatt gaacatcttg gcgataccat tccgcttgac ctctggtttt
    42961 acaatgaaaa cagtgagcag gttacactcc gctcactcat cgaccggcct accatattga
    43021 gcttcgtcta cttcgactgc ccgaaccttt gcagccccct gatggacggc attgccgatg
    43081 tggtaggcaa aatggacatg accctgggta ctgactacaa gatcattacc atcagcttca
    43141 acaccagcga tactccggag aaggcaaagg aaaagaaaca aaacttcgtc cagcggataa
    43201 gcaaggagaa ccagaaggac tggatttacc tgacgggaac tcaggagaac atcagcgcca
    43261 tcacggcagc tgcgggattc aaatacactc ctcaggggct tgattttgcg catccttcgg
    43321 tgataacggt ggtcagtccc gccggcaaga ttacccgtta cctgtacggc cttacatact
    43381 tgccgtttga cgtaaagatg gcagtgattg aggctcagaa ggggattgcc agaccgactg
    43441 ccaaccggat cctcgagtac tgtttcgctt acaatccggc cagcaagacc tacaccctgc
    43501 aggtaacgag aatagtgggt attattacga ttttcttcct tgttctgata atgataattc
    43561 tccttgccag gagaaggaaa aaataagagg gattcttatg ggaaatgaaa cacagccgag
    43621 ttacctggag taccagggca gacacaaggg attggcagga tggctgctaa gcaccgatca
    43681 caagcggatc ggactgctct atctctattc catcgtggtg tttttctttg ttgcagcttt
    43741 ccttgggctg cttatgcgca tcgagctgat agccccggga aagaccatca tggagccaca
    43801 gacgtataac gggctcttca ccattcacgg aatcatcatg atcttcgtga ttgtgattcc
    43861 cggcctggct gcagtcttcg ggaacttttt cctgccgata ctgataggcg ccagggatgt
    43921 ggccttcccg aagctgaacc tcttctcatg gtatctctat ataacgggag ccgtgctggg
    43981 ggttatgtca cagttcatgg ggagcggtcc tccggataca ggctggacct tctatgttcc
    44041 ctacagcgtg gagtcggcca ccagtgtgat atgggcactg acagcagctt ttatcctggg
    44101 attctcgtca attctcacgg gtctgaactt cattgtgacc attcaccgga tgagggcgcc
    44161 ggggatgaag tggttcagga tgccgctctt tccgtgggct ctttatgcaa cggcatggat
    44221 acaggtactt gcgacaccga ttgtgggtat cacgctgctg atggtgatag ccgagagggt
    44281 gctgaacatc gggttctttg atccggcgct gggaggtgac cccgtgctgt tccagcatct
    44341 cttctggatc tattcccacc cggcagtata tatcatgatc cttcccgcca tgggtgtggt
    44401 cacggagatt tttccgacat tcagccagaa gccggtattc ggatacgggg caatagtggt
    44461 ttccagtctt gctattgcct ttgtaggcta tttcgtctgg ggtcaccata tgttcacctc
    44521 agggatgagc tattatgcaa ggtggttttt ctcgttcctt acgtttatcg tggcggtgcc
    44581 cagtgcaata aaggtattca actggatatc aacaatgtat ggaggttcga ttgatctcaa
    44641 gcctcccctg ctctatgcca tctcgttcat cttcctgttc atgatcggcg gtttcaccgg
    44701 cctggtgctc ggttccctgg ccaccaacgt acagactcat gatacagcgt ttgtggtggc
    44761 acactttcat tacataattt tcggggggat gggtttcgcc ttctttgccg gcatgcacta
    44821 ctggtacccg aagatgttcg ggaagatgta caacaaccgg gtggcgaaca ttgcatgggg
    44881 attcaacttc gtcggattca acctgcttta cttcccacat ttcattatcg ggatccaggg
    44941 tatgccgagg cgttcatacg actatctgcc acagttccat acaggccatt tcttatccac
    45001 catcggggca ttcattctga ttgcagggct gttgctgatg ctgtacaatt tcatcagggc
    45061 cgggaggcgt ggtgaaaaag cgccggccaa tccgtggcat ggcgtcacac tcgagtggaa
    45121 gataccctct ccgccgcccc atgagaactt tgatgagata cctgagataa catcgaggcc
    45181 gtatttcttc ggaagcaagg aaaaaacaaa ccagtagtca cccatgactg tacatccgac
    45241 acatacagaa catattgaca gtgaaaccgg taaactggga atgtggatat ttctgtttac
    45301 tgagctgttt ctctttggcg ggctcttcct ggtctatgct attttcaggg cgaagtattc
    45361 cccggagttt catcatgcgg cggaagagct gaatgcgttc atcggaacaa tcaatactgt
    45421 attcctgctt gtcagcagta tgaccgttgc cggtgccctc acagcgatgc agaaaggtga
    45481 caggaggatg actctcctgc ttctcacagt gaccatcatg cttgccggcc ttttcatggt
    45541 caacaagtat tttgaatggt cgcacaagtt cgagtacggc atctttcccg gttcgccggt
    45601 actgaagaac atgcctcacg gcgaactcct cttcttcggg ctttactaca tgatgaccgg
    45661 gttgcacgca ctgcacgtac tggtgggcat ggtgctgctc tcgataaacg ttgtgaaggt
    45721 aaaggccgga actgtcaaca gtgaacgttt cctgatgctt gagaacagcg cgctctactg
    45781 gcatcttgtt gacctgatct ggattttcct tttcccgctt ctttacctta taacctgagt
    45841 atgagatgag agacgaaaaa aaccacatat cgacctacag gtcactggta acagtactgg
    45901 tgatacttct ggcgctgacg acaatatccg tgttgattac agggattcat ctcggtccgg
    45961 tcacagtggc cgttgcgctc ctgattgcaa gtgtcaaggt ggcaatagtg atcacccagt
    46021 tcatgcatct caaattcgag aatcttttcc tgaagctggc agtgtcaggg gtgttcacgc
    46081 tcttcgtgct tgtcattgtg ataacgttca ttgattactt tttcagataa acaattaagt
    46141 tatgttcaca ggtgcatcaa atcttgcaga aggggtagac aaggcgttcc tgttcatctt
    46201 tatcatcgcc tttatcttta tcatcgggat ctcggcattc atgatctgga ctgtcattca
    46261 ttactcaagg aaaaagggca gggaggcgaa gcagttcagc ggcagcatga aaatggaggt
    46321 attgtggact gtcattccca caatactggt gatgctgatg ttctggattg gctggaaggg
    46381 atttgccccg atgcgcaagg tgcctgccga tgcgatggtt gtcacggcga taggcaggat
    46441 gtgggaatgg gaatttgttt acagtgacag cctgcgctcg aaggagctgg tgttgcccat
    46501 caacaaaccg gtgaagcttg acctggtggc tgaggatgtc aatcacagcc tcttcatacc
    46561 ggctttcagg gtaaaggagg atgtgatacc cggttatgac aactggctct ggtttactcc
    46621 caattacata ggtgactacg agatactctg cacagagtac tgtggcctgt tgcattcggc
    46681 aatggtatca aagacgcgga ttgtggagca ggaggagttt gaccgttggt atgaggagct
    46741 gaaggtggca tcatttatgc cggagcacga aggactgaag ctgttgagga gtaccggctg
    46801 cctggcatgc cactcccttg acggatcaaa actcgtgggg ccctcatttg caggacttta
    46861 tggctcgcag cgcgaggtgg tctccggtgg ttcagcaaaa agcgtaaccg ccgatgaggc
    46921 ttatatcacc aggtcaattt atgatcccaa cggcgagatc gtagagggtt acgcgaggaa
    46981 cctgatgcag tcatacagtg agttgctgac tgaagaacag gtgggtatca tcactgaata
    47041 cctcagggaa ctggttgaag gtgacgggca gaaggaggct gacctctgac aatgcagaga
    47101 cttccggtat gaagacaaca aggcaaccgg tatgtgacat tgcggagctt tcaaggtgga
    47161 agctctcgct ggcggttgct ttttccgcag tgacgggtta cttgctcttt tccggcggag
    47221 atccgggcgg agcagtattg acggcagctg gtgtcttcct tctctcggca ggcgcctcag
    47281 ccctcaacca gtatacggag cgtgagagtg atgcgctgat gacccgcact gcgggcaggc
    47341 cgttgccctc ggggagaatg gcgccgcctg ccgccatgct ggcggcggcg gtcatgcttg
    47401 ctgccggaat cgcgtttctg gccggcggcg gcacgtggcc ggtgttgctg ggattactta
    47461 atgttgtatt gtacaacgtg atctacaccg gcctcaagaa gaagaccaca cttgccatca
    47521 tacccggagc ccttgtcgga gccgtgccgc cgctgatagg ctatacggcg gcaggcggcg
    47581 ccatagccga cacacgcatc attctctttg cactcttcat gttcctctgg cagatgcccc
    47641 acttctggct cctcctggta cgttacggcg aagagtatga aaaggcagga ataaaaacgc
    47701 ttcacccggt catgagtgac aaagccataa tgcggctcgt gacagggtgg ataaccggct
    47761 cctccctcct gctctggata cttactgcca tcttcatgcc attcagccgg acggcctcca
    47821 taatcctgct tctgctgaat gcggctttca tcctcggttt tgccaggata accgcgattg
    47881 gttccggcag aaccggacca gagtacacat tcatggcctt caactccttc tcggtactgg
    47941 tgatggtgat actgattgct tttgcctgac ctgtcagtaa gcctgttaat ctcccgattc
    48001 attcatctgc aaaaacaggt tgtccctttt tgggcaattt cgctaacttt attctctcat
    48061 tttaatcaaa ctcagatatg aagaagacac tttccatttc cctgatgctg atggtttgca
    48121 tcgcgacatg gtcgcagccc ctgaagtctg atgagataga caagcttgtt aaccgtacgc
    48181 tggagacctt taatgtgccg ggcattgccg tggccgtggt aaaagatgac caggtagtcc
    48241 acatgaaggg gtacggtgtc atttcaatta ccaccggcaa aaagacagat gaaaacactc
    48301 tttttggcat tgcatccaat tcaaaggctt tcacctcagc cgctctcggg atcctggttg
    48361 acgaaggcag gatcacatgg gaaacgaagg tcattgacat cataccggag ttcagactgt
    48421 ataattccta tgttaccgag gatttcaaca taaaggacct gctgacccac cggagcggga
    48481 tgggtcttgg tgcgggcgac ctgatgctct ggcctgactc atccgcattc accacggagg
    48541 agatcatcca taacctgaga tacctgaaac agacctcgtc attcaggacg aaatatgact
    48601 atgacaacct gctatacctt gttgccggcg aggtggtcag gagggtctcc gggcagggat
    48661 gggatgaatt tgtggagtca aggatcatga aaccgctggg gatgacagag tctgcagcct
    48721 cgctcaacag ggttagagac aggtcgaata tgattgatgc gcacgttcct gtggaaggtg
    48781 ttcttcaggt tgtgcccatg tatgcaagca cccttcttga ccctgccggc ggcatcatga
    48841 gcagcgtggc tgacatgagc aaatgggtga tgatgcagct caaccgggga aaatacggcg
    48901 aaggcaacag caagcagatg ctctccgaga gggtgcatcg tgagatgtgg acgccgcaga
    48961 ccatcatacc ggtccgcaat cccggtcctt acaggagtca tttttcggcc tacggccttg
    49021 gctggggtct caccgatgtg gcaggatacc tgcaggcatc acataccgga ggactggcag
    49081 gcatagtaac ccaggtcacc ctgataccgg agctgaagct gggaataata gtattcacaa
    49141 accagcagga aggatctgct ttcactgcca taaccaacac catcaaggac ggttatctgg
    49201 gggtaaaggg gaacgactgg gtaaaaaccc ttaaggaaaa tgttgaccgg aaccgcgatg
    49261 aggcaaagaa gataaccgac gaaatatggg caaaaattga ggcacagcgc ttaaaaagtg
    49321 cttctgcggg aagcaatgca atttataccg gcacttataa tgacgtgtgg ttcggagaca
    49381 taattatctc ggatgaggga ggaaaactgc gtttccggtc tgtcaaatca ccgaaaatgc
    49441 gcggagagat gctttactac accggcaaca cctttgtcgt gaagtgggat gacaggtcta
    49501 tggatgccga tgcatgggcg gtcttcacgc ttgataagga aggcaaggct gaagcattca
    49561 gtatggatgc catctcgccc ctgactgact tcagctatga cttccaggac ctaagtccga
    49621 agaggaagga ggtcagatga ggagagcggt ttcactgatc ctgctcctgg cccttgcggt
    49681 cgcggtttcg ggtcagcccc tgtcgtcaaa gaagattgac aggctgacca cgagggtgat
    49741 aaaggagttc aatgtcccgg gtgtggccgt ggccgtggta aaggacggaa aggtgattca
    49801 catgaaggga tatggtgtcc gttcaattgc ctcaggtcag aagactgacg aacatactct
    49861 cttcgcgata gcatcgaaca ccaaggcgtt tactgctgcc acgctgggta tcctcgtaga
    49921 tgagggcagg ctcagatggg atacgaaggt gactgagata atccctgaat tcaggctcta
    49981 caactcatat gtaaccgaag acttcttcat aaaggatctg ctgactcacc ggagcggtat
    50041 gggtgaaggt gcgggcgacc tgatgggatg gcctgattcg gcaatgttca ctgcggatga
    50101 gataatacat aacctcaggt atctgaaaca ggcgtcatct ttccggacca ggtacgacta
    50161 cgataacctg ctgtatattg tggccggcga agttgtgaag agggtctcgg ggcagagctg
    50221 ggaggagttt gccgagtcac ggatcatgaa acctctcggg atgaacgaat ctgctgcttc
    50281 cttcaggcgg ataaaagacc ttaccaacat cattgatgcc catgtgatgg ttgacggcag
    50341 gctgaaggtg gttcccaaac aggaagccaa gaggcacaac tcggtgggtg gcatctacag
    50401 caatgtcagt gacatgagca agtgggtcat tctgcagctc aacagcggga ggcatggcag
    50461 cagtgccggg gagaagatct tctcggtgga ggctcacagg gagatgtgga cccctcagac
    50521 cgtgaaaaca gtcagtacat cggggtcata caggagcaac ttcgcattct acggactggg
    50581 atggaacctc accgatgtaa acggatacct gcaggtctat catacgggaa gtcatgcagg
    50641 cattgagaca agggtgacaa tgcttcccga actcagcctg gggattatcg tgttcaccaa
    50701 ccagcaggtg cccgaggcac atgaggcaat caccaattca ataattgacg gctatctcgg
    50761 gataaggggc aatgatcata ttatgaaact cagggagaag gtggaacggg cacaggctat
    50821 ggcaaggaag attaccgatg agatctgggc aactgtagag gaaaagagga aggagtcatc
    50881 tccggggccc ggaaccgggt catttgcagg gacctacagt gatgcctggt tcggcgatat
    50941 aattatcagc gcagaggatg gccggctaag gatcagggct gagaagtcac caaggatgag
    51001 gggcaggatg ttccactata cagccaacac tttcattgtg aagtgggatg acaggagcat
    51061 ggatgccgat gcctttgcgg tattcaccct tgacagggaa ggaaaaccgg tttcgtttac
    51121 catggaagcc atctcccctc tcactgattt cagctatgac tttcaggatc tttgcctcgt
    51181 cagaagggaa aagtgacccg ggttattaga caaccggcgc ataaacatta ttttagcacc
    51241 caaatcagaa aaccgaaccg atgaccttta ttcatttgag cgtctggatc tccgctctga
    51301 taggagttat catcattgca tggatccgca gcttcgatat ctatgaaaaa gagacattca
    51361 ttgccatgct gtgggctttt cttgccggcg gcatcacttc ggtcattgtt gcccttggag
    51421 cctatgagat tctaaggatt tttggtcttg atgatgaggt cataagcaac actctgggtt
    51481 cgctgctggt aataggtccg gttgaggagt tcgcaaagct tatcggcctg gtggttgttt
    51541 attcactgat caggaagcag ttcaatgagc tgactgacgg gatcatctac atgtcatgcg
    51601 tggcgctggg gttttccatc attgagaact acttctatgc caactctggt gaaaacagcc
    51661 agtatctgct tgtctacagg gcttttataa gcacaccggc acatatatca ttcagtgtca
    51721 tcatcggtta cgcctggtac aggtacaaga aggagaacaa gccgttcggc acagtgatag
    51781 ttgcattgct ggttgccagt ctccttcacg gggtctttga cgccctggca ttcagtccgt
    51841 ggttcaactt catgttgctt gtttatctct ggctgataat caggcaatct ctcaggctgg
    51901 tgcagtacac gaatgtcatc tcgcctttca ggcctggttt tgaagcactg ctggaggctc
    51961 cttcgggtga gacagctgac ggtatcgagt gtccgaactg cggttcatcg gcgcccaagg
    52021 agctcttcag aaacagtttc ttcaccgcct gccgctgtga caactgtggt taccacatag
    52081 catcacgcaa tgatatcaga aagatattca ggatatttgc tcccgagtac aaaaggcttg
    52141 caaagaagct tgtgccggcc aggttcagtg acggccgtac cgtaatgagc gtttatggct
    52201 ctgccttctt tggcaacagc ggcaatccag ggtttttcag ggtaagtgat gttgccggaa
    52261 ggttacaggc catcaatgat gacctgctgg acaggttcag gaagcgatca ttcatatcag
    52321 ccaacctgct gaagcggttc tttgaatgaa aaatgagtaa ttgagttatc ggagattcag
    52381 gatgtacact cttttttaca acgcacgctt tttcaccggc acaggcttgg ttgacgggaa
    52441 agaactgatc gtcagggata accgtattga aggttttgcc gatcagggct cagtgtcagc
    52501 agatgcgcac cgcattgact gcggtggcaa cctggtggtc gcaggtctga ttgaccttca
    52561 gatagcggga ggaggaggtt accttttctc ttcaaatcct gcccctgaag cacttgatat
    52621 gataatcaga tcgatagtct cggccggcac aaccggattt cttctcgcgc tgcccaccaa
    52681 cagcagggag gtatacaggg ctgccttcct gacagtctca ggttgtacta atccggcagt
    52741 gatgggcctg catatggagg ggccgttcat aagcaatgca cggcgcgggg cgcacgctcc
    52801 tgagctgata atgaagccgg ttccggaaaa tgtaacttca cttcttgacg aatcgggcgg
    52861 cacgatcagg atgatgacag tggcgccgga gatatgcact ccggaggtga ttacccttct
    52921 cagggagcgc ggcattaccg ttgccgccgg acacagcaac gccacattca gggaagcaat
    52981 ggcaggcttt gagatgggga ttgaaaccac gacgcatttg ttcaatgcga tgtcacctct
    53041 gcatcaccgt gatccgggtc tgcccggggc agcatttctc tctgaaacag catgtgcaag
    53101 tatcatcgca gacggaattc atgtagacta tgccatggtg agaattgcca gtaagataat
    53161 gaaggagagg ctgtttctcg tatccgatgc cgtcgaggag aatgaccgcg gagcatacag
    53221 gcatgtaaag aaatctgacc gttttaccct gcctgacggc actctttcgg gagcgaagct
    53281 gacgatgctt gatgcagtaa gaaacagcat caacagggca ggaatagaaa agtacgaagc
    53341 cttcaggatg gcaacgctgt accctgcaag gctgataagg gccggtgaca ggggaatgat
    53401 tgagccgggt gccagggctg acctggttat tgtggataat gagatgaaac tcaggggagt
    53461 ttgtttaaac ggaataactg aatttaaaaa gtgataaaat ggcattgctg ggaatagatc
    53521 ttggagggac gaaggttgcg tttgcgcttt ttgaccgcgg gggcaacatc agggcaaggg
    53581 aggttttacc cctggatgac cgccggggag gtgaagtggg gaagctgatt gccgtcaatg
    53641 cggcgctcaa gcttgctgcc gccgaaggcg agggtgatcc ggtggaggcg ataggcatat
    53701 cagtgccagg catcagccgg cgcacgaagg ggtcggtatg ggcccccaat attcaaggct
    53761 gggatgacta tcccctgctg aaggaggtta caacagttac aggcaggatc ccggtcatta
    53821 ttgagagcga caggtcatgt tatatctcag gtgagatatg gaaaggtaat gcccgcggct
    53881 gccgtgatgc gattttcctg gctgtaggaa cgggaatagg tgcaggcatt gccgttaacg
    53941 gcgggttgtt gcacggagct gatgatattg ccggggcaat aggctggatg gctctgtcaa
    54001 ggccattcag gagcaagtat accatctgtg gcaacttcga gtatttcgcc tcaggtgaag
    54061 gcattgcccg ccaggctgca gaatacctga agaagcatga aagataccag ggagacctga
    54121 gaaaaggtcc cggtgcgaag gtcaccactc atgatgtgtt cgctgcacgg gagaagggcg
    54181 atgttgcggc ctcggctatc attgacctgg ctgtggagta ctgggggatg gcagcagcga
    54241 acctggtaag tcttttcaat cctgaaaaaa taatatttgg cggcggggta ttcggccctg
    54301 ccgttgagtt gattcctgcc atttaccagg aagcggagaa gtgggcgcag ccgataagca
    54361 tacggcaggt aagttttgag ccctcggcac tgggtggtga cgccggtgtt tacggggcag
    54421 gataccttgc catgaaacag ataggttaac cattcccatg actatgaaag ggacttataa
    54481 cagacaactc gtattctgga cagcatgcct tggcatgctt cttttcggta tcggactgat
    54541 aatgcttggg tccgtgctgc ctgacctgag ggtaaggcat tcgcttgatg cggttgaggc
    54601 cggcactctg ctgtcagtac tccccatcgg gattattttc ggttcacttc tcttcggacc
    54661 ggtatgtgac cggtacggct acagggtgct tctcgctgtg tcggcgctgc tgatgtttgc
    54721 cggatttgag gggcttgcct tcacccgcac cgcatggatc ctgacactgt cagtcttcct
    54781 cttcggtctg ggtgggggag ccatcaacgg agccaccaat gcagtggtgg ctgacatcag
    54841 caccacgggc aagggtgcgg cgctgagcat tctcggtgta ttcttcggca tcggggccct
    54901 gggcatgccg ctgctgctca gtctcctgaa agccaccttt gagttccagg taatagtggc
    54961 cgctacggga atgctggcac tggtcgcagc ggtgctcttc gcagttgtca ggttccctgc
    55021 agccaagcag gcggagggta tttcgatgaa gcaggtcggc gccctcttca gggatgtcac
    55081 ccttctgctg attgcatttt ttctcttctt ccagagcagc ttcgaggggc ttatcaacaa
    55141 ctggaccacc acctacctca tggaaagggt tgccgcaccg caggaggccg cactgatggg
    55201 gctttcggcc tatgttgccg ggatgacggt gatgcggctg ctgatagggt cagtgctgag
    55261 aaagatgcct gagaagcagc ttctctatat ctctttcggc atcacccttg ctgcactgct
    55321 tcttttcagc ctcagccgct cggtgatacc ggcagcggcc gggctcttca tgcttggcgc
    55381 cgggcttgcg gcaggatttc ccaccatgct gggaatcgtc gggaacaggt atcccggtct
    55441 ctcgggtaca gccttcagct tcgtgctggt catagggttg ctgggcaaca tgcttgttaa
    55501 ctactccatg gggatagtgg ctgaaaatgc cggcattaag catctcacca cattcaccta
    55561 tgccgagctg gcgatcctgg tggtcatggc aacggctatt tttggcagac ttaagatgaa
    55621 caaataaata acaaacaact aaaaacaaca gatatgcttg cacttgaatg gctgaataat
    55681 gccagaaatg tgatgaataa catcgaggag acacagatgg agaatatacg caaggctgcg
    55741 gaagtgatgg ctgacaccat tgaatgcgga cggtgggttc acactttcgg atgcggccat
    55801 gccacactgc cgatagagga gatgtatccg aggattggcg gctttgtggg ttttcacccg
    55861 atgatagaga tcccgctcag tttctttacc cacattacgg gagagatggg tgttcaccag
    55921 ttcgtattcc tcgagagggt tgagggttat ggcgccgaga taatgaaggg atacaccttt
    55981 gacacgcgtg acaccatgtg gcttttctcc cactcgggga tcaacaacgt gaacatcgac
    56041 attgccctgg aatcgaagaa aaggggcatg aaggtggttg tgttcggctc agcagctgcc
    56101 gcgaagggaa agaagacaag gcattcctcc ggaaagacca tctttgacat agctgacata
    56161 gtggttgaca cctgtgcacc gctcggcgac gcctcagtag agctcaagaa tcattatgac
    56221 aggattggtc ccgtctccac aatggctttt gtcacctgtg tctggatgac aatcacaacg
    56281 gttgcagaga tactggctga caggggggtt aaactctaca tacatccgag tcacaacgtg
    56341 ccgggagaca acaccgccag ggagaggctt gatgaggccc tggccgagta caagcgcagg
    56401 gttgcaggag tctgaaaggg gaacaccatg aagccgcttg acaggaccat aggatttatc
    56461 aggggcactg ctgttgacag ggtgcccttc catcccatcc tgatgcgttt tgcagcgtgt
    56521 catgcagggg tgcggtaccg ggacttctgc ctcagtcccg ctcacaagtg catagccaat
    56581 atcaggtgtg ctgaagattt caggtctgac tgggtaaaca ccatgtctga tccgtgggca
    56641 gaagctgaag ctttcgggac agtactctcc taccctgatg atgatctgcc gaaggtggag
    56701 cgtcatgcga tagaagagat atctgacatt gacaggatga ccgtgctccg tccgggtgat
    56761 caccggcgga tgctggcccg tgtggaagag atagagatat accggagaga cactgccggc
    56821 gtgcttttca tctgcggctg ggttgagggt ccgcttgcgg cctggtgtga catcagggat
    56881 atcaatatgg cgatgactga cctttacgag tatccggctg aggttcataa agcgctggat
    56941 atcattactg aaagcgccat ggctttcatc accccgcagg tcagtgccgg ggctcactgc
    57001 atcggtatcg gtgattcggt ctgctcactc atctccccgg agctgtatga tgagttctgc
    57061 ttcgaacgcg agaagaacct ggtggaacac atacattcac tgggagcgct ggcaaagatc
    57121 cacatttgcg gcaatatatc ggctatcctt ccgcgggtaa tcggcacagg tgctgacata
    57181 atagatattg atcaccggac aggaccggtg acggaagtgc ttggattatt gatgcccgga
    57241 caggtttttt cgggcaagag tgatccggtt tcggtggtcc aggacggcga cacggaatct
    57301 atccgcagca gttgccggtc attctttgat gaggcaggag agcgtgcaat cctctcggcc
    57361 ggatgtgagg taacgccagg tacaccggcg gaaaacctgc gctttttctc cgggatggcc
    57421 ggggaacttc tcccggctgc cggacgtaag gtttaagctg agttgaaggt ttcaggtcat
    57481 tattagtaac aggataataa actgcagaac aaaatgagca ggaatgtggc actggttctc
    57541 tccagcggcg gggcaagagg cttcgcccac atcggcgcca taaaggtgct ggaggataac
    57601 ggctatacaa tcacctcggt ggcaggcacc tccatgggag cgctggtcgg aggcatttat
    57661 gccgcggggc ggttgaagga attcgaggaa tgggtcagcg aactcgacgt gatggaggta
    57721 ctgaaactga ccgatataag catcagcagc aaaggattcg tcaaggggaa cagggtcatt
    57781 gagaagatca gggagattgt ccatgaccat aacattgaag agctccggat accttactgc
    57841 gcagttgcaa ctgacgtgat ccggggacgt gaacggatct tcacctccgg cagcatgttc
    57901 aatgccatca gggcatcgat atcaatccct acattttttc agcctttcag gatggacaac
    57961 gactattatg ttgacggcgg cctggtcaat cccattccca tcaaccgggt aaagcggata
    58021 aagggagatc ttctggctgt tgtcaatgtg aatgcagaca tcccgcatgt aagtatcggg
    58081 actgcggatc aggatgagaa cagggcgaag gggtatctca ggaggataag gaacatcaat
    58141 gcgcatgcag acagacatat ccctaaaaac aagaaggatg acatcggcat attaaatctc
    58201 aaccaccgca gcataagcat catgcttaac cagattgctt cactgaccct gatgaacaac
    58261 catgttgaca tcatggtaca ggtgtcaaag gaatcatacg gaatctttga tttttacaag
    58321 gctgctgaga taatcaggga aggtgagcat gccaccgcac tggccctggg agtacaattg
    58381 ccgtgacgtg ctgtcaggcc ttcacttttg caggtaaaaa ttgtagattt tcgttaaaag
    58441 aacatatctt tgactattga tctaaactaa ttccagatga aacgatccta tctcatgtta
    58501 tgcctggtac tgttgattac ggcgccggca atgttcgggc agaaaccgat aaaggtgctt
    58561 gaggacagta ttcagtttgg caactacctt tatccgggat tcaatgttac cattcccgaa
    58621 gccaactatg acaagatact caaggactgg ataaagaccc aggaaagcgg caccaagtcg
    58681 aaggtacaga ctgagggcgg agagatgacc attttcgggg ccatagtcaa ggaggtctcc
    58741 cctgccccgg tcaatatcta cagcaggata atgaatgaag ataccctcag caggatgctg
    58801 gtatgcatag agctgaagaa ggaccagtat gtggaagctg cttcaggcga cattcagctg
    58861 acctcagcaa agaattacct gaaagagttt gcaaagaggc agtatatgga cttcataaag
    58921 gaggagcttg ccgccgagga aaagatactg agagagctca aacaggacct tggaagcctg
    58981 gagaacagta aagcccgtac ccagaggacg gccaggaaca acaggaacac cgtgaacgac
    59041 gaacaggaga agctgcttgt caagcataat gagctgagcc ttctgtcaaa tgagatcata
    59101 aacaagaaca acgagatgat agcaatgccg gtgggccccg gcagggatgc catggccacc
    59161 caggtgaagg aactcgagaa gaggcgcaag aagcttcaga aggagatcag caagggagag
    59221 agccgtataa acaaggccag gtcagccatt gaccaggctg acagggctat ccccaggaat
    59281 gatgatgaac aggctgtcat gaaatcaaag attgatgcac agcaggctgt tgttcagacc
    59341 ttcattgaca agctgaacaa cgtcaaactc tactgatcct tcatcggaca attttaattc
    59401 attaaccaat atatcaaatc aattatggaa aaccaggaaa aaaaagagac ctaccagcag
    59461 aagatacagg aacagctgga cgaatggaga gcagatattg acagactgaa agaaaaggca
    59521 aaaaatgcca cagccgaatc aaaactgaaa taccaggaaa acattgacaa gcttgaggtc
    59581 aagatggacg aaggcaagtc gaaactgaaa gaccttaagg aatcaagcgc tgaggcatgg
    59641 gatgccgtga aggatggagc tgattcaata tgggatacca tgaaggcaac ctttgccgag
    59701 gtcaaggaca ggctgaggga caaggacgac gacgacgata agaaagcgtg agtactttct
    59761 tcaaagccgg cagttgacag ttggcagttg gcagtagtga gattttgtag ttaatatgtt
    59821 gtttatcaat gtattacatt aatatgtgac tactggttgc tgattgcaga ctgccgactt
    59881 cagatataat tttatactga caaatatgaa aaaatttgta acaataggaa ttgttgtact
    59941 gctgattgcc gctgcggtta tcttctggtg gcgtcattac tttgtatttg gagagggtgt
    60001 aaaggccggg gacctcaact accttgtcag gaaaggctat gttttcaaga cctgggaagg
    60061 caagctgata cagacaggct tcaagacacc gactcccgga gccattcaga gcaatgagtt
    60121 cgagttcagc ataacgaatg acagtctggc cacggtactt gaaagagcca gtggcaaatt
    60181 cgtagagctc cgctacaagg agtacattgc accgcttcca tggcgtggga tgagcaaata
    60241 tgtggtaata gagatccttg acataaagga ttctgaacag gagcaacaga ccctcatacc
    60301 ctaccaggca atctgaaacc aacaagacat aaaaactgat gaccagaaca agaacagcag
    60361 cactggttgt gttgctgatt atgctgtccg gatgtgctga attactgaac accctgaaga
    60421 ccatatcggc ggtgaagcag cttacggaag cggatatcgt ggcggggctc agggaggctc
    60481 tcagcgtcgg agcgcgcaat gcagccgggc ggctggcatc gcaggacggt tactgggggg
    60541 atccggcagt caggatacca ctgcctgacg aagcgaaggt aatagttgaa aatatcggaa
    60601 ggatacccgg gggacagcag ttgattgaca atgtggtact gagcatcaac agggctgctg
    60661 aggatgcagc ccgcgaagtt gctcccatat tcgtgaattc agtgacacag atgactatca
    60721 ctgacgggta caacatcctg cacggtgctg acaatgcggc cactgtttat cttagaaaca
    60781 ccacatggaa cgggctttat gatctctaca agccgaagat cagcgcatcc accggcaagg
    60841 aaattgtggc aggcgtctca gcacaggatt catggaacac cttgacggga aaatggaacc
    60901 aggtggccaa cagtgtggca ggaaggctgg ccggattcaa accggtcaat accgatcttg
    60961 acgatttcct gactcggaaa gcactcgagg gagtctttct taagcttgaa ggtgaggagc
    61021 tgaagatcag gaaagaggtc tcggccaggg tcactcccat cctcagggaa gtattcggga
    61081 cgcttgacca gcaataagac actcccggac tgtttgatgc aggctcaaca agtttgcacc
    61141 tctgttgtta tatttgcaca ctaaatctga tacaaatgag aaaaataact attgtactgt
    61201 cacttgcact ggcacttaca ggaataaacc cggcggcatc cggccaggat gccgacacca
    61261 cccagggttg gcacaagagc ggagtattct ccctgaacat gtcacaggca aacttcacca
    61321 actgggctgc cggaggtcag aactccttgg ctctgaacgg actggttaat cttggagcaa
    61381 actacaggat gggcaagtca gcctgggaca atgcccttat aataggttac ggaaagatgg
    61441 ttcagaaagg gaatgacctg ggatggatga agacagatga ccgtatcgac tttcagtcaa
    61501 agtatggtcg gaaggcaact gaaaagtggt tttattcggg tctgatgagt ttcaaaacac
    61561 agatggacaa cggatacaat taccctgata cggataacaa gatatcgggt ctgctttcac
    61621 cggcctacct gctgttctcg ttgggtatgg actacaagcc caatccgaat ttttcggtgt
    61681 ttctctcgcc gctgacatca aagaacacca ttgtcatgga tgatgatctt tccatggcgg
    61741 gtgctttcgg agtggaaccc gggaagaatt tcaggccgga gctcggtgca tatgcaaaca
    61801 ttgcctacaa gaaggacgag attgtcaaga acgtcaattt catgacaaag cttgatctct
    61861 tcaccaatta cctgaagaat ccacagaata ttgacatcag ctgggaagcg ctgctggttc
    61921 taaaggtcaa cgaattcatc tctgcaactg taaacacgct cctgctctac gacgatgaca
    61981 tactcatcaa gattgacgag ggtagtgaag gtgagcctgt ccttggcaag cgcgcacagt
    62041 tcaaggaggt catcggcgtg ggtttcacct acaagttcat taaatagaat gtactcatat
    62101 tacgcgacag catgaaaagc ataacacttt ttgcaattat gacgcttatt actggaatcc
    62161 tgacagggca ggcggtgcag gacatcaagc tgaatgcccc tgacatggag agagggctta
    62221 caatcatgaa ggcatttgaa aagagggctt cggccacctc cttcagttcc agggatctct
    62281 ccctgcagga tctctccgac ctgctctggg catcaaacgg catcaacagg ccggaaagcg
    62341 gcaagcgcac ggctcccact gctgtcaatg cacaggatgt tgacatttat gtgctgctca
    62401 ggtccggggc ttacctgtat gatcacaaga ctcatactct aaagggtatt gctgccggtg
    62461 acaacaggaa gctggtttca gacaggcaga ccacgtttgc caatgcgccg gttatgcttg
    62521 tgctggtctc tgacgtatcg cgcttcaggg gtgatgatga tgcccgcaag ctgagctggg
    62581 gtgcaatgga tgcaggaata gtctcacaga atgtcagtct cttctgtgcc ggggcagggc
    62641 ttgttacccg tccgcgtgca tcgatggaca ttgaggggct gaagaagctg ctccagctga
    62701 acggcaccca gcacccgctg ctcaacaatc ctgtgggtta tcccaacgag tagcggctgc
    62761 ccggtatgct taagctgata aatcatcccc tgatcacgca caagctgtcg cttatcagga
    62821 ggaaggagac gggtacacgt gatttccggc agaacatcag tgagatagcc gggctcatgg
    62881 cgtatgagat caccagggac ctcccgctcc gcgagatagt aatagacact cccctttcat
    62941 catgctccac ggctgagctt tcgagagaga tcatactggt gccggtgctg cgtgccggtc
    63001 tgggaatggt tgacggaatc actgccctca ttaccacggc gcgggtaggt catatagggc
    63061 ttgtccgtga ccatgagacg cttgcaccta tggagtatta cgccaagttc cccgctgaca
    63121 tttcagaagc ggttgtgatg gttctcgatc ccatgctggc aaccggcgga agtgcaagcg
    63181 cagccatcag cgcagtgaaa aaaagaggag cggtgaccat caggctggta tgcatcgtgg
    63241 gcgccccgga aggtgtgacc agggtcgaaa aggatcaccc tgacgtggat atttacctgg
    63301 cagcgcttga cagtcacctg aatgaaaacg ggtatatcgt tcccggactg ggtgatgcag
    63361 gagaccggct attcggaaca gtttagttta ctatattcgc agattcacag gaaaaaaaca
    63421 ggattatggg acttaagcgc cggcttatca ttatgaactt cctccagttc tttatctggg
    63481 ggtcatggct cctgaccata ggagcatact ggtttcagaa caaaaactgg tcggggaccg
    63541 atttcggagc catcttttca acaatggggc tggcctccct tttcatgccg gcaatagcgg
    63601 gtatcatagc agacaggtgg atcaacgcgg agaagcttta tggtattttt cacatcgcag
    63661 gggcagtact tctctcactg ataccggcaa ttaacaatcc tgacaccttc ttctgggtgc
    63721 tgctcctcac catgatgttc tacatgccga cactggccct ggcgataacg gtggcctaca
    63781 gctcgatgaa gaacagcggg atggatatta tcagggatta tccgccagtg agggtctggg
    63841 gcaccatagg tttcatcgtg gcgctgtggg tggtgagcct gctgggattc gaaacctctg
    63901 cgttgcagtt ttacgtggct gctgccgctt ccctcatact tgggctttac gcttttaccc
    63961 ttccaaagtg tcctccgctg gcaaaaggtc ataagaagaa gcttatcgag gagctcggac
    64021 tcaatgcatt caagcttttc aagagcacca agatggctct cttcttcatt ttcgcaatgt
    64081 tgctgggtgg tgcgctgcag ctcaccaatg cgtacggaga cactttcctg catgattttg
    64141 caaagattcc tgcctacaag gatcttcttg cagtgaaata cccggccata attatgtctg
    64201 tgtcacagat atcggagacc ctttttatcc tgaccattcc atttttcctg aagcggttcg
    64261 ggataaagaa ggttatgctg atgagcatgg tggcatgggt actccgtttc gggctgttcg
    64321 gcgttggtaa cccggcagaa gggctctgga tgataatcct atcgtgcatc atttacggga
    64381 tggcatttga tttcttcaat atttccggtt cgctcttcgt ggaaacccag agcactcctg
    64441 acatacgtgc aagtgcccag ggacttttca tgatgatgac caacggtttc ggggcatttc
    64501 ttggcagccg catcagcgga atagtgattg acaaatactt catcctggag ggcggagcat
    64561 ttgactggaa gggaatatgg tttgcctttg caggatatgc gctggtggtt gcggttctgt
    64621 tcgccatatt cttcaggcac aaacatgtgc cggaggagac agcttccgtg agtcactgat
    64681 tctttgtccc ggaggagaca gctaccatga gtcagtgacg ctttgcccct gagcgggctg
    64741 gcgcaggctt ccttcgagtc acttattcca tccccctgag cgggcacgca caggtttccg
    64801 gacagctgat gaaggcatgg agaggcttca ggcaggtgtg attacccgag caggatcagg
    64861 ctgataccaa tcattatcat cccgcttatc aaaccataga tggcaacatg gtgtttgcca
    64921 tacttgtggg cggcagggag gagttcatca aaggagatat ataccataat accggcaacc
    64981 atggcaaata catagccaag gcttatgccg gtattgaaga gcgtcaggat gaaatatccg
    65041 gccagtgcgc caatcggttc cgagaatcct gacagaactg accatgcaag cgccttcctt
    65101 cttgaaccag tggcataata gacgggcacc gacacggcga taccctccgg aacattatga
    65161 atggccacag ccactgcaat gctgatgcca acagtcacat cagacatggc tgacatgaat
    65221 gtggcaatcc cttcggggaa attatggacg ccaatggcaa tggcagtgaa tgttccgatc
    65281 cgcatcagct tccggtccct gatgctgtgg cctctgcggt tctggttccc cgtgtcatgc
    65341 ccgccacggt tctgattctt tctcccctgg cattccggtt tatccgggtc gcgtgattcg
    65401 ggatgcatct gctctaccct cttcagttca tggggattgc cctctgacgg caggatcttg
    65461 tctatggctg cagacaggcc aatgcctgcc acaaaggcga tgaatgtcag ccaggctgca
    65521 gtattggcgt cataattctg cctcatgatg tcgccggctc cggtaagtat ctcagtgaat
    65581 gagatgaaaa tcatcactcc tgctgagaat ccaagggaaa aagcaagaaa tgtggtcttg
    65641 gtacgtttgg caaagaaagc tatcaatccg cctatcccgg ttgcaaggcc ggcaaagaga
    65701 gtgattccaa atgcaaagag gagccgggaa ctatccatca gaaggcgtta tttagattga
    65761 gtttaaattg caaaagtaaa agaataacgg gaaaaacaac atgaggggta ttcctgctga
    65821 taaaaaaaga ccttattttg atattgcaat taacgatgaa tctctgggat tactatagga
    65881 tactcggcgt taagcagaat gccactgatg atgagataag aaaggcgtac aggaagaagg
    65941 caatggagta ccatcctgac cggaacagtt cacctgattc ccaggagatg tttatccgca
    66001 taaccgaggc ctatgagtac ctgatatctc atccctaccg gcggaatatc accgaggagg
    66061 aggtgaggcg caattaccag gcatggattg attaccggag agaggaggcg cgcaaacgtg
    66121 cagaggctta tgccagagcc tcatatgcag agttccggaa aagcccgatt tacaaaagta
    66181 cgacagtcat tgacggcacc attgtcttcc tggggctcat tctggcaaca atggtcatat
    66241 tcacaacgat ctacggctac atttatcgaa tgaaagtagc cgattctcca aaggaggaac
    66301 cctcaataac gctggccgcc gtatcactga tcataggcat ttcgtatatg atcatttccg
    66361 taatatacct gagtgcatgg attgcccagc aaaagaagaa aaggagaata tctgaagatg
    66421 gagcacagaa agaagataag gaatccgttt gatgctgacc agaacatgtg tttcggatgc
    66481 ggtccgaaaa atcatgtcgg gctgaagctg ttgtttgagg aggatgatga gaaggtatat
    66541 gcatcatggg tgccggacag gcaattccag ggttacatca atgtgctgca cggaggcatc
    66601 attgccaccc ttctcgatga ggtcagcgcc tggtgtgtct atctgaaggc ctgtacctcc
    66661 ggcgtcacct cttcaatgac ggtcaggtat ctcaggccgg tacatatcag caaaggcact
    66721 gtcaccattg aggcgatgat aacgaagaag ggagaaaaga atgcgctgat ctcagcagcg
    66781 ctgcacgacg gtgaagggaa gctatgtgct gaggctgagc tggactattt catttaccct
    66841 gagaagattg cccgtgaccg gtaccactat ccgggcaggg aggcattcat gtatcaatcc
    66901 gtgccaccgg gatcaccgag tggtcagtga attctgccct caggtacttg tagtaccctg
    66961 taataccgat cattgcggcg ttatctgtag tatacctgaa ttcagggagg aaaaggttcc
    67021 agcccctttt ttcagcctcc cgtgttgctg cacgtctcag gccgccattg gcagagaccc
    67081 ctccggcaat tccgatctcc ctgatgccgg tctccctgga agccctcacc agcttgccaa
    67141 tgaggatctc gataatggtc ctctgcagtg aggccgcaag gtcattgagg ttcttcccga
    67201 tgaaaccggg gtcctctgcc accctgtcac gcaggaaata cagaaaactt gtcttcagtc
    67261 cggaaaaact gaaattaagt ccctgtatcc gcggttttgc gaagcggaat gcgtcagggt
    67321 taccaacgga tgcaagccgg tctatcaccg gtccgcccgg gtaagggagt cccatcacct
    67381 tggcgcattt gtcaaatgcc tcgcctgctg catcatcgat ggtattgccg ataagttcca
    67441 tgaccagatg gtcgcgtatg acgatcagct gagtatggcc gcctgacacc aggaggcaca
    67501 ggaacgggaa ttgcgggata cggttttcct ttccgtgttc catggcgaag tgagacatga
    67561 cgtgtgcctg gaggtgatta acagcaatga gggggatgtt tcgcgccagc gcaaaacctt
    67621 tggcaaatga tgttccgacg aggagtgaac cgagcaatcc gggtccctgt gtgaaggcta
    67681 ctgcgcttac ctcatcagct cttttccctg cccgggcaag cgcctggtca accacaggta
    67741 ctatgtttgc ctggtgggct cttgaggcaa gttcgggcac caccccccca taggccttgt
    67801 gtacatcctg gttggcgatc aggtttgaaa gcacgaatcc gtcgcagatc accgcggcgg
    67861 atgtgtcgtc gcaggatgat tctatcgcga ggatcgtaat ttgtggtgcc atttttgtat
    67921 cactcttttt tataacttcg ccagcggcgg gaatcacttt gtgaatggcc aaaattatta
    67981 aaaaaatcgt cagagtcctt ctcatcatag cagcagtgct ggttgtggtg cccctgattc
    68041 tcttcctgct tcttcaggcg ccgcggattc agacctggac ggtaagcaaa gtgacgaatg
    68101 cagtctccgg gaaaacaggg gctgagatct cgataggcag ggtttcctac tcactgtggc
    68161 atgagattgt gcttgatgat gtgctgttca gggacctgaa cggcgacacc atcgttgcag
    68221 cccgtaaggt tgatctgagg ctgcgtgaga tcagaccgtc acacaggatt tttaaattcg
    68281 gaacaattga cgttcacgag cctgatttca ggatgatcaa ggacactgcc ggggtcatga
    68341 atctgacagc ctacctcagg tcattgaaga gtgaccggga gcgtgacagc acagggggca
    68401 tcgacatacg ttttgctgac attgacgtgc tcaacggatc ctttacactg gctgacatgt
    68461 ctgatacgat cggggttgtc gcgggcagtg tcaactacat gaacatgcac atcagacaca
    68521 tcaccggaaa agtaagggat ctcaggataa tacccgattc tgtaagcatg gtgataagag
    68581 ggctggcgtt tcatgaagcc ggcggatttg taagcaggag tcttgatatg aacatggctg
    68641 tgacagagag cagcctctat ttcagggata ttgacctgct cactgactca tccgccattt
    68701 ctgctgaaaa gatcctgcta atgccccgtg aagaggactc atggaaggat ttcatcaatg
    68761 atgtaaggat tgacttcctg ttcaataatt ccatcgtaaa tacctccgac atctcgtatt
    68821 ttgtcaggcc ccttgcgggg atcagtgagg aagtcagctt ctcgggcagg gtggccggta
    68881 ccgttgcaga gctgaaaggg aagaatatca ggattgacta tgaggcctcc accaggctga
    68941 ggttcgattt tgacatatcc ggactgcctg agataaacga cagttatctg ttcatcagtt
    69001 tcaccgacat gaaaacccgt gccgcggata tcgaacggtt cacggtgccg ggaaggaagc
    69061 ctctcaggct gcctgaagtt gctcatgacc tggggctggt cacatacaag ggaagcttta
    69121 caggattcac cacagatttt gtatccttcg gcaggatcac caccgaaagg ggtacgtttg
    69181 acacagacct ctcacttaag cctgacgggc gaaacacctt cggattcagg ggttctctca
    69241 gaaccagggc tgtagatctc gggtacatgg caaagaacag tgagatgttc ggcgggctgt
    69301 ggatgcatgc agaggttgac ggaacaatga agtcattccg ccatctctca gcaaacatca
    69361 acggggtgat agacagcgtc gaggtaaaca aatacctgta caggaacatc agcgtctcag
    69421 ggacctatgc cgacaggatc tgggacggca gtgttgaggt gaatgaacca aatattgaga
    69481 tgagccttct cggcaggttt gatcttgaaa agagccagcc ggagtttgac ttcaccatga
    69541 accttgccga tgctgacctg cacactctca accttgtaaa aaaagattca gtcttcaggg
    69601 caactgcgct tgtcactgcc aatttcaggg gcaactcaac ggaaaacctg gaaggagacc
    69661 tgaggctcat caactcgact cttgaaaact caaacgggaa gctcaacata tatgacttcc
    69721 ttgtcagttc cgaaatcgtc agcggtgaac ctgtcctgag gctcaggtca gattttgccg
    69781 acgctgatgt gagagggcag tactccgttg atgccataaa aagtagcgtc tcagagataa
    69841 tggcgggcct tttcccttcg aggttcaata agccggtagc aaagaaaggt gagtcaacac
    69901 ctgaagctat gttctccctg gatgccagga tcaagaggat tgacaggctg aatgagtttt
    69961 tagggactgg ccttggtatc tcggaaggga cgcatgtcac aggtcacttc tggtcagacc
    70021 gccctgaggt tatagcaggg attaaggcag atgccgtaac atacatgggt ataagaatgg
    70081 gccggctgaa tatgacaggt tcgttaaagg atggcagcct gaagctggac atgactgcag
    70141 atacagtgat gctgcctgac aaatccgagc tggggaattt tgccctcggc gccacaagct
    70201 gcaatgacac aactgacctg ggcatgaact gggacaatca tgacaacggg cgtacgctgg
    70261 gagaggtcaa ggccagggga tttttcagtc atggtgtcgg caatttgccg tcactgacgg
    70321 ttgcggtgct tcctaccggc ctgactgtca acagttcaca gtggaatatc agtcctgcca
    70381 ggatcgtaat tgactccgtc agcaccttct tcgacaatat actggtaagc agcaggacga
    70441 gatatttcag tctcggaggc agtctctcga aagaccccga ggagaagctg accctggctt
    70501 tcgaggggct taacatgtca tacctgaaca acctcctccg gggaaagcag gatgagacag
    70561 aggaaccgac ccagatgaag tttgaaggaa ttctgagcgg caatctcacc ctgtcagatg
    70621 tttttaataa cctgcttttt gagagcagga taaatgtcac tgacttcagg ctgaatgaca
    70681 acaactatgg cctgatgacc ataatcagcg aatgggatcc gaggagaaag gtggcagtaa
    70741 taaatgtggg caatgacttt gccgggagga agctcattga cgtcagcggt acctattcgc
    70801 cgtcggcgcg tgtgattgac attgcagcat cggcatttga tctcccgctt gacgtgttaa
    70861 atccgtttct cagttctttt gcttcggata tccggggttt cggaacggga accatcaggc
    70921 tgaaggggaa gccacggcag ctggagatga ccggagcggt aatggcgcat gatgcctcga
    70981 tgaagattga ttttcttcag acgcgttaca gtttctctga cagcgtcagg ttttcgcctt
    71041 caggcatcat cctgaggaat atcaatattt atgatgcaca gaagaaccag ggcatgataa
    71101 acggtatgat cacacaccgt gcattcaaga attttggtgt gagccttgac atcaatatgg
    71161 acaaaatgct ggttctcaac accaggccaa aggacaatga cagtttttac ggcaccgcct
    71221 atgcttcggg ttatgccggg ataaggggga atgatgagaa gctggttttc aatatctcgg
    71281 cccggacggc tccgaacacc gaattctatg tgccgttgaa ctcatcggcc agtgtaagtg
    71341 attatccttt cattgagttt ttgaaacctg cgggggagac cggcgagcag gagcagccag
    71401 acaggcagag cagttttgtt aggcaggaag agggaagcaa aatggagctt aacttcgacc
    71461 ttgatgttac gcctgaagcc gaggtacagc tcatccttga cgagacagcc ggaggagtca
    71521 tcaggggcaa gggtgaaggc ctgctgaaca tctccctgaa ctcaaggggt gacatgcgaa
    71581 tggccgggga ctatgtgatc agtgacggag attacctctt cacccttgga agcatactca
    71641 acaagaggtt tgcagtggaa gagggaggga cagtctcgtg gaacggggcc attgaagatg
    71701 ccagcctgga tataagggcc ctttacaaga caaaggcatc gctctcggag atcttcgggg
    71761 aggaggaatt tacggagctg aaagccaaga tgccggtcga atgcattctc agtctctcgg
    71821 gaaggctcct gaatcccgca atcagttttg acatcagcct ccccaccgct gacgagcgta
    71881 cccgtgagtt gctgagaatg gccattgaca ccgaggagga gctcagccgc cagtttcttt
    71941 acctgctggt aatgaacagc ttttaccctg atccttcgct ttacaactca ggcactacca
    72001 caggcacacc ggtggctcag gggggatatg ccgtatcacc gatcgtaggt gtcacaacca
    72061 ccaccgagat gctctccaac cagctcagca actggctgtc acagataagc aatgattttg
    72121 acataggctt cagctacagg cccggaagtg agctcaccga ccaggaggtg gagctggctc
    72181 tctcaacgca gttgctgaat gacaaggtta ccgtcaacgg gaacgtagac gtgcggggaa
    72241 accagtcaaa caccacggcc agcaacataa gcggtgagtt caccgtggag gtcaggctga
    72301 ccgatatgct caggttcaag gtattcaatc gttcgaacca taacctgtat taccaggttc
    72361 atccatacac ccagggggtg gggctctttt acaggcgtga tttcagcacg ctgaaagacc
    72421 ttttcatcaa accggaggaa aagaagagaa aaaacatcga gagtgaaggg gaaaccgggc
    72481 agaactaaca tttcaacaat gaccccgttt tatcaacaat gggatactta caggggatga
    72541 aatccgtttt ttactatatt cgcgcgatga tacgcgaaag caagtcaaac tgttaacatc
    72601 ttaaaccaag gaaaatgatc tcagcagcta ttttattaca gggaacacca gccactgaag
    72661 ccgcagaaga ggcaacaatg tcactggctg atctttacct ggcaggagga tgggtgatgt
    72721 atctcatcac actgctctct tttgttgcac tttacatttt tatcgaaagg tacctggtga
    72781 tcaagaaggc atcaaagggt gatgccaact tcatgaaccg cataaaggac tacatgtatg
    72841 aagggaaggt tgatgcggcg ttgcagctat gcaggaagac caactctccg caggccagga
    72901 tggttgagaa ggggatcacc cgtctcggca ggcctctggc cgatgtgacg gctgcaatag
    72961 agaatgtggg caggctggag gttcacaagc ttgagaagag gttcccgaca ctggcaacaa
    73021 ttgccggagc cgctcccctg atgggattct ttggaacggt cataggtatg gtacaggcat
    73081 tttatgaaat gtcactggca ggaaacagcc ttgacataag caccctcagc ggaggcatct
    73141 acaccgcgct cattaccacg gtgggaggtc tgatacttgg gattctcgcg tatttcgggt
    73201 acaaccagct ggttgtaaag gttgaaaatg ttgtgttcca gctcgaaaca acatcaaccg
    73261 agttccttga catcctcaat gaacctgtaa aacagtaact gatggcaatt tcgaacaaga
    73321 ataaagtcag tgttcagttc agcaatgcag gcatgtctga tattgtgttc c
//
LOCUS       DAOPXU010000002        72755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_231_length_72755_cov_12.601045, whole genome shotgun sequence.
ACCESSION   DAOPXU010000002 DAOPXU010000000
VERSION     DAOPXU010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 72755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 72755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72755
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_231_length_72755_cov_12.601045"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1639
                     /locus_tag="PKK03_00350"
     CDS             <1..1639
                     /locus_tag="PKK03_00350"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX82866.1"
                     /translation="ADMNKKILIFGIILSALMISSCVKDLDVTPKDPTKILAGNLGDN
                     PEYMKMALGKLYASFIISGQGANGGDDIAASDGNFFTTMRALWNLQEITTDEAICAWG
                     DVGIADLNTQTWSPSNPFLTALYQRLALSITYANDFINNAQEYGGANADRYVAEARFL
                     RALAYYWAIDLFGQFPFLTEADGVGKFFPQIGTRGDIFNYVESELLDIENKLGAPKSS
                     YPQADQASAWMLLARLYLNAEVYTGTPRWDDCREYCDKVIASNVYSLATNYRQNFSAD
                     NDGRHNPEMIFAWEQDGIHVQGYVGTTFIIESSSDATYLKAEDYHGLTSNTNWNGNRA
                     RIQFLAKMVDTLEVYDNQPIPASDPTLSLSPDKRVFLKIKKSRNIPSPSASGDYGIGV
                     YKFTARNADGTLPSNYNAAYACTDFPVFRLADAYLMRAEALYRMGGEANIAAAVADIN
                     KVRERAYGNTSGNITVADLDLDFIIDERGREFYYEAQRRTDLVRFGKFTGGDYLWQWK
                     GGAYTGASTSEHLNLFPIPGDELSSNPNYNGVNNPGY"
     gene            1658..2704
                     /locus_tag="PKK03_00355"
     CDS             1658..2704
                     /locus_tag="PKK03_00355"
                     /inference="COORDINATES: protein motif:HMM:NF025650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusE domain-containing protein"
                     /protein_id="HNX82867.1"
                     /translation="MKKNILIYLTFIGLAAFFTSCEKDGEKVIMLSDPTAPVLTTVPD
                     LTFQRANGMNVLEFVGTPVDPGFQASATYYLEACASGTSFADPVTLWTGNQCKSMKAT
                     VSDLNGILIKKFPADASSALDLRLRAVLVVDAGTGAPGTGTNTFTYISDLKSATAALY
                     GLPRLNLIGSGMTQKIESALGDGKYTGLVKFDITKPFTLNDPDAGTNYGATGTTLAVN
                     GAAIVPTANGWHILTVDVNALTYDISEYRIGLVGSATPNGWDSPDQAMEYNPATGTWS
                     ITITLADGEIKFRFNNAWAWNLGYRPGGKDPADLFHNGDNIAVTAGNYTITLTITQPN
                     GPDEAGSCIIVKNN"
     gene            2739..4298
                     /locus_tag="PKK03_00360"
     CDS             2739..4298
                     /locus_tag="PKK03_00360"
                     /inference="COORDINATES: protein motif:HMM:NF027726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusF/SusE family outer membrane protein"
                     /protein_id="HNX82868.1"
                     /translation="MKSILKIMMLPVAAVLILAGCTQETSEIRLDPKLGTTKVFNITS
                     NSATVTGFVVAEGEGFTTRGVCYDKQPAPTTSKSTAPFAGPAINAAYNVTLTDLDFAQ
                     TYFARAFATGPAGTVYGEELTFTTLPVVATVTTVDATDVEGTTAVSGGSVTADGGSAV
                     TARGVCFGTTTKPTIDAPNGKTVDGAGLGSFVSNLSGLKGLTKYYARAYATNSAGTAY
                     GNEITFTTLVSVREWYVPGDYVAASYPGSGLADWAPDKSPMVKSNEANPNNVEGYVYM
                     ANASNNFKFASQPNWDGPNYGASGTAGVLSSDPNAGNNNLPQGFYKLNVNAGVDPMTY
                     TAVAMTWGVIGDINGWSAQVPLTYDPALHVWKGAMHMPVGGWKFRANDNWDYNYGKGT
                     GSNDLVAGSQDNLSITVADDYAITLDLSQPLAYTYRADRWGIVGSATGSWDVDQNMSW
                     DAVNKVFIATIDLTAGEIKFRANDDWGYNFGGSLTDLSSDNAPNIPIAEAGNYKITLD
                     PWARVATVTKN"
     gene            4420..7239
                     /locus_tag="PKK03_00365"
     CDS             4420..7239
                     /locus_tag="PKK03_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNX82869.1"
                     /translation="MKIKIPVLVLIFAGWSLAVSSQVITTDPVFALASAPVVITFHAD
                     RGDMGMKDYTGDDVYAHTGVITNLSSGQSDWKYVVSGTWSTTVPPPDKVKLTRVDANT
                     YTLTISPSIRAFYGVPESETIQKMAFVFRNGNASHTGRDNNGADIFYNVTVEGSYEIL
                     LSQPALYTSLVNTGDVVPVSFSASVCDSLILYQNGVQLKKVTETTLSHTVTAGGSGSY
                     KLLVKAWHNNVMKADSAYYFIRTPTAVAPVPAGLKPGVNITGDNSAAFLLYAPYKSSV
                     FVIGDINNWIHSSEGFMKRSPDGNWYWLEVTGLDPDEEYGFQYLVDETLRIPDPYTTK
                     VLDPWNDKFIDESVYPGLKPYPEGLTNELVSVFRTRPPEYPWKNNDFTVPARDTLVIY
                     EMLVRDFVATHTFKTVRDTLDYLKRLGVNAIELMPVNEFEGNSSWGYNPSMYFAVDKY
                     YGTPDDLKELIDECHGRGIAVIMDIVLNHSYNSNPLVRLYWNSTNNQPAANNPWFNVT
                     TPHYYSFGGSDFNHESNATKALVDSVCHYWIDEFRFDGFRFDFTKGFTNKTSNSDYSV
                     SAYDPSRIAILKRMGDRIRAYKPDALLILEHFADNAEEKELAAYDFFLWGNGKWRYED
                     ASTGNSSDISDASWKLLGFDRPGVIDYMESHDQERIMYLNLTQGKAEGGYNIRDFNTA
                     IKRVKLTATFFLTIPGPKMLWQFQELGYDVILGDGYQRLEEKPVRWDYYTVAVRKNLF
                     DNFAGLIELRKKYPAFSSDNFTLYQSGMTKRLNIVHSDMDVVVLGNFDFFPRTIDPNF
                     TRTGNWYEFFRGTQVEVNASNQNTPISLVQGEYRIYTSKQVTRPSFLLGMEDPLADDG
                     EGLMFDVYPNPFRERAVIKFAGDDQYQPHTVEVISATGAVVQIMNTPAGIDDVILDGS
                     ALDAGVYYLKVTAGRVSAVKRVVRY"
     gene            complement(7386..8756)
                     /locus_tag="PKK03_00370"
     CDS             complement(7386..8756)
                     /locus_tag="PKK03_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNX82870.1"
                     /translation="MKEHIFKILILFIGAATLMVACRPKPAAVIEPLKTTVVHADWTR
                     NMVLYEINTRQFSDEGTFAGVQERLPQLKELGVNVLWFMPIYPIGELNRKGELGSYYS
                     ISDYKGINPEFGTIDDFKALVDTAHAMGFHVILDWVGNHSAWDNPLATEFPEWYEKDS
                     LGKFISPFDWTDVIQFDYTAEPLWDYMVGAMRYWVEEVGIDGYRCDFPGLVPEEFWFR
                     ATTELNSVRPVLMLAEDEEHSYLLERAFDMNYGWAHHHLMNAVAGGQRRPSALDSIIQ
                     YELKRYAPESYRLRFMTNHDENSWNGTIDEKMGEAQKAFACYLFTVPGVPLLYNGQEA
                     DLDKRLEFFKRDPIEWKETELTPFYTKLVHLRTSHPALRHGIEGGSYDVLKTSQKNVV
                     AYKRVKGENEVLTILNISSKPVTVALKDGATAGTYRDLFTDGSVEIAQGRKMQLEPWG
                     YMVLVK"
     gene            complement(8767..10608)
                     /locus_tag="PKK03_00375"
     CDS             complement(8767..10608)
                     /locus_tag="PKK03_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HNX82871.1"
                     /translation="MKRIFQALVLFMIPVLLPAKGQVIERVDPPSWFTGMKNDALQLM
                     VYGREIGSYDVKTDYPGVDVSTLVRTENPNYLFVNLNISDEAVPGIVTLTFTHGRQKL
                     THSYPLLARPDGPARGFNSSDVIYLLMPDRFANGDTSNDNVAGMTEQADRSKPGGRHG
                     GDLKGIEDHLDYLHMLGVTGIWLNPFLENNQQHSSYHGYSTTDFYKSDPRYGSNDEFK
                     NLVTEAHKRGMKVVMDMIFNHTGSFHWWMKDLPSKDWIHQFDEFTRTNYRASTYMDPY
                     AAESDRMLMEKGWFDVTMPDLNQSNPLVETYLIQTSLWWIAFSDLDGIRMDTYPYPQP
                     DMMSRWAKRVTDEFPGFFIVGEVWYDDPAQISYWAYNKKNSDGYRSNLPSVTDFPVCF
                     ATHRAFEGKTNPTDGLSKIYNALSQDYLYPEPLRNVIFLDNHDMTRFYTQTGKSLEAY
                     KLGLSFIMTTRGIPQLYYGTEIVMEGDKSLGDGRLRDDFPGGWPGDARNVFTGTGLTE
                     QEKEALDFTTLILNWRKNKEVIHTGKLKHYIPSDGVYVYFRYDCSESVMVIINANSKE
                     NRAISGEKYAGSLEGFTSGTDIISGRKIDDLKSFTIAPQTAMIIELK"
     gene            complement(10608..12920)
                     /locus_tag="PKK03_00380"
     CDS             complement(10608..12920)
                     /locus_tag="PKK03_00380"
                     /inference="COORDINATES: protein motif:HMM:NF010380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 65 glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HNX82872.1"
                     /translation="MTDSRFQPDEWKIIETRFDPVRGRDSESIFALGNGMMGQRANFE
                     ETYTGDSLRGNYVAGVYYPDKTVVGWWKVGYPEYFAKVPNAPSWTNIIVRIDDEELDL
                     VKCKVKSFRRELDMKEGVLTRMFTAVMPSGREAEVTARRFLSMDEPELAAISYSVRIK
                     RGGGKLELIPWLNADVYNEDANYDEKFWENESASRDGRRSVVVAQTRKTAFVVATAME
                     NIFTMNGKPVKGEPVITTTDKTAHCAFTSACADGDTFTVTTYAAVLSSFNHPVDGIAA
                     KAMATAGKAAAKGFDALLEAHRNAWAQKWEHGDIVIRGDIAAQQGIRFNIFQLNQTYT
                     GEDPRLNIGPKGFTGEKYGGSTYWDTEAFVFPFYLSTSDTHVARNLLLYRYKHLGKAI
                     ENAKKLGFTGGAALYPMVTMNGEECHNEWEITFEEIHRNGAIAYAIFNYVRHTGDQDY
                     LSEYGLEVLIALSRFWAQRSNWSQEKNRYVILGVTGPNEYENNVSNNWYTNTLARWTL
                     KYTSDILDWLRSASKVKYNAVVTRTHLDTQAELNHWKEIIDNMYLPAHHDLGIFLQQD
                     GFMDKLQQTVKDLDPAERPLNQHWSWDRILRSVFIKQADVLQGIYFFEDDFDEDTIRR
                     NFDFYEPRCVHESSLSASIHSVLAARLHDTESAYTLYLRTARLDLDDYNREVKDGLHI
                     TSMAGTWLAIVEGFGGMRIRNERVCLNPVIPAAWESYSFIIRYRNNPFRVEVNGTKVI
                     VVNLSQAEIPLAVYGEQVTLKTGEERRFEK"
     gene            complement(12927..13577)
                     /gene="pgmB"
                     /locus_tag="PKK03_00385"
     CDS             complement(12927..13577)
                     /gene="pgmB"
                     /locus_tag="PKK03_00385"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HNX82873.1"
                     /translation="MMDLKGCIFDLDGVIVDTAKYHFMAWRRLAKELGFIFTLEDNEA
                     LKGVSRMASLEILLSKGNIHATIQEKEELAARKNGWYVEFISGMKPDEILPGSVKLLK
                     TLRKEGILTAIGSASKNAGTILDGINLRDMFDVIIDGNKISKAKPDPEVFLRGAQEMK
                     LSPSKCIVFEDAQAGIEAAIAGGMKCVGVGSPELLGRADLVIPGLNKISLKQLRNL"
     gene            complement(13577..15118)
                     /locus_tag="PKK03_00390"
     CDS             complement(13577..15118)
                     /locus_tag="PKK03_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX82874.1"
                     /translation="MNVIYRRKPRLSGWQIWNMSFGFLGIQFGFALQNGNVSRIFQTL
                     GAEIDNIPILWIAAPVTGLLVQPIVGHMSDRTWNRLGRRRPYFLTGAILASLALAIIT
                     NSPTWIFATVMLWIMDASINISMEPFRAFVGDMLSSEQRTSGFAMQSFFIGVGAVIGS
                     FLPWILANWFNVPEVPAEGKLIPFNLKLSFYIGSAVLLLAVLWTIIRTKEYSPAELEE
                     FEKGEKTARGEPLAEKTVSGQTETVRDINFYRPGIIWFSVGLVLTIILSQVNLEKELY
                     VVTGGISLFGLLQIIAGFLKAGGKGSNGLNSILTDLKHMPATMGQLAVVQFFTWFALF
                     SLWIYTTSAVTSKIYGTSDTASLAYNEGANWVGVLFGVYNGFAALVAFLLPVLAKYTS
                     RRVTHAICLLVGGVSLMSISLINSPGMLILPMLGIGLAWASILSMPYAILTGSLPHNK
                     MGIYMGIFNFFIVIPQILAASILGSMVKHLFSGNTMLALVTGGISMIIAALAVNFVKD
                     VDDRR"
     gene            15460..17070
                     /locus_tag="PKK03_00395"
     CDS             15460..17070
                     /locus_tag="PKK03_00395"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNX82875.1"
                     /translation="MGDFNYEKPFQHSRDVTKYRRISVDHVRMTECCGRKILQVQSEA
                     LRTLARQAFVDVNFYLRTMHLEKLAMILRDPEATDNDRFVAWVMLRNSVISSEGKLPW
                     CQDTGTAIVMAWKGEDVYTGIDDALHLSRGIWETYNEKNLRYSQIVPSSMFAEKNTGS
                     NLPAQIDIASCPGDEYRFLFIAKGGGSANKTFLYQQSKSLLNEASLSRFIREKIEDLG
                     TAACPPYHLAIVIGGTSAEMTLKTVKLASAGYLDDLPTSGNSAGRAFRDLEWEERVEK
                     ICQEYGVGAQFGGKYFVHDVRVIRLPRHAASCPVGIGVSCSADRNIKGKITAEGIFIE
                     QLETEPGKFLPEKAPELDKPVVINLERPMKEILAELSKYPVKTRLSLTGTLIVARDMA
                     HARIKQMIDEGKPMPDYFQNHPVYYAGPAKTPEGMASGSFGPTTAGRMDPYVELFQSL
                     GGSMIMLAKGNRSKQVTDACKKYGGFYLGSVGGPAAILAAENIVSVEVVDFEELGMEA
                     VRKIVVKNMPAFILTDDKGNDFYSGIKQ"
     gene            17288..18952
                     /gene="asnB"
                     /locus_tag="PKK03_00400"
     CDS             17288..18952
                     /gene="asnB"
                     /locus_tag="PKK03_00400"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HNX82876.1"
                     /translation="MCGVVGVFDLKIRAGELRQQVLGMSGKLRHRGPDWSGIYCGEKA
                     IIAHERLSIVDVQSGRQPLYSRNRKLILGVNGEIYNHREIRERYRDSYDFTTKSDCEV
                     ILALYADRGCDFLDELNGIFAFVLYDEENDCYLAARDHIGIIPFYMGWDMYGNLYFAS
                     ELKALEGVCKKIETFPPGHYLYSRDGVMKKWYNRDWTSYDKVEENSTDIVALREALEK
                     AVHRQLMSDVPYGVLLSGGLDSSVISAIAKKYAARRIETDDQADAWWPQLHSFAIGLE
                     GSPDLAAARKVADHIGTVHHEIHITVQEGLDAVRDVIYYLETYDVTTVRASTPMYLMA
                     RVIKSMGVKMVLSGEGADEVFGGYLYFHRAPGPREFHEETVRKLSRLHLYDCLRANKS
                     LAAWGVEGRVPFLDKEFLDVAMRLNPADKMAGNGRMEKWILRKAFEDYLPDDIVWRQK
                     EQFSDGVGYNWIDTLKQLTSEKVTDEMMSTARYRFPLNTPMTREEYFYRSIFTEHFPS
                     ETAALTVPSVPSVACSTAEALAWDEAFRNQNEPSGRAVRNVHIKSW"
     gene            complement(19032..19736)
                     /locus_tag="PKK03_00405"
     CDS             complement(19032..19736)
                     /locus_tag="PKK03_00405"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNX82877.1"
                     /translation="MEEKIYSMPRIGDKAPEFKAVTTQGEINFPSDYSGSWVILFSHP
                     ADFTPVCTSEFMTFASMAAKFEKANTKLVGLSVDGLYSHIAWLRTIKEKIEYKGMKDV
                     EVKFPLIEDITMSVAQKYGMMMPGESSTKAVRAVFFIDPKGVIRTILYYPLSLGRNFE
                     EIYRVVLGLQTADAFSVALPADWQPGDDVIVPTAGSCGVAKERMESKEEMKCYDWFFC
                     TKKLDKQKILDTILKK"
     gene            complement(19778..20278)
                     /locus_tag="PKK03_00410"
     CDS             complement(19778..20278)
                     /locus_tag="PKK03_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNX82878.1"
                     /translation="MAEIGRSIVRMDVEELLTLLNKALADEWLAYYQYWIGAKVVKGP
                     MKDAVIAELELHATEELTHAQLLVGRIIQLGGTPVLSPNEWTKITNCGYDAPEDPFVS
                     VVLDQNIKGEQCAIRVYNKLLALTREADPITYNIILQILTDEVEHEEDLVALKEDFDL
                     MIERRK"
     gene            complement(20326..21798)
                     /locus_tag="PKK03_00415"
     CDS             complement(20326..21798)
                     /locus_tag="PKK03_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HNX82879.1"
                     /translation="MKKKKLTTAAGRPYFENEDSMTAGPRGPVLLQDYYLHEKLAHFN
                     RERIPERVVHAKGTGAFGTFTVTGDITKYTRARLFSKVGNKCRVFVRFSTVGGEKGSA
                     DTERDPRGFAVKFYTEEGNWDLVGNNTPVFFIKDAKKFPDFIHTQKRDPKTNLKSDTM
                     QWDFWSLNPESLHQLMTLFSDRGTPDGYRFMNGYGSHTFSMYNKKNERVWVKFHFKTQ
                     QGNKTLTRQQAEEIKGKDPDYSQRDLVNAIAGKDYPKWTLKIQVMTDAQAKKFKWNPF
                     DVTKVWPHKEFPLIEAGVMELNEIPENYFADVEQAAFAPAHTVDGIGLSPDKMLQGRI
                     FAYPDAHRYRLGANYQQIPVNRPIAPVSHHQRDGHMTVDGNQGSGPNYFPNSFDNIEA
                     DKNYREAAMALDSDVADYFDRNKNDNDHYSQAGALFRKVMTDQERANTVGNLVCALKG
                     VSGPKKKEILMRQLEHFHKADKELAQLVAKGLGIKFPRAK"
     gene            complement(21813..22187)
                     /locus_tag="PKK03_00420"
     CDS             complement(21813..22187)
                     /locus_tag="PKK03_00420"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HNX82880.1"
                     /translation="MIRLLLISAGTVSLALGIIGIFIPVLPTTPFVLLSAGLYVKSSP
                     ELYRRVVNGRITSAYVTPRAQKKAGIWAMAIMWTMILLTFFLWVSNIWLKTALIAVGV
                     TGTIFKIRYFFSQRNNKADSNH"
     gene            complement(22184..22624)
                     /locus_tag="PKK03_00425"
     CDS             complement(22184..22624)
                     /locus_tag="PKK03_00425"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNX82881.1"
                     /translation="MKIEDYGTRLRESGLRVTPQRLAVYEAVDVLHDHPTAEEVSQYI
                     RKKHPEIALGTIYKTLETLAEKAILRRVKTDSGLLRYDAVDDRHHHIYCTHCDRIEDY
                     YDAGLTKMLYDYFEIHRIPGFRIEDIKLQIVGKYTDNRETIRKR"
     gene            22964..24985
                     /locus_tag="PKK03_00430"
     CDS             22964..24985
                     /locus_tag="PKK03_00430"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNX82882.1"
                     /translation="MANRKSLALAALLLIHTAFVNAQVLEPVAWSFKSEKTAENNYDI
                     VMTATIDNTWHLYAMDLPEGGPVATSFTFETPVGYTLEGKPVAVNQPEVKFDNSFGMD
                     IGMHSGTVEFRQKITVSKLPVTVSGFVTFMSCDDTQCLPPRDVEFSVNIGGEVAASAA
                     SAEQAAGTTVPADKPRKGFLKFFLLSMLAGFAGILTPCVFPMIPMTVAFFSQGTGSKG
                     SAIGKALIFGLSIILLYSSLGIIVSLTSAGAGFANTLSTHWIPNLLFFTLFLVFAASF
                     FGAFEIVLPSKWVSTSDSKVDRGGALAAFFLGLTTVLVSFSCTGPIVGALLVEAASGD
                     VLRPTIGMFGFGLAFALPFTLFALFPSWMQKMPKSGGWLNSVKVVLGFIMLAFSLKFA
                     STVDTVYNLGILSRDIYLAIWIVLFVLLGMYLMGKIKFSHDSDVPHIGIFRLVLIIAS
                     FTFALYLVPGLFGAPLTRISALLPPRETSGFNLLKAIGENRGTAAPGALVPGTLAPGT
                     LATAGAALCEEPKYGDFLHFEYGLQGYFDLEQGLACAKKLGKPVLIDFKGHACANCKE
                     MDARVWSDPGVQQRLRDNFVLVALYVDDRTKLPESEVYVSKVDGKQKKTMGKKNEDIE
                     ISMFNTNTLPLYAIVDPDGKPLTETRGTNFDIQAYIDWLDRGVKAFRNQ"
     gene            complement(24988..27261)
                     /locus_tag="PKK03_00435"
     CDS             complement(24988..27261)
                     /locus_tag="PKK03_00435"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNX82883.1"
                     /translation="MKGRLLTTLIISMATVISQGQDADILKYVNPFIGTDKMGHTYPG
                     ATVPFGMVQLSPDTDTLPYEVNGSYNRDVYRYCAGYQYADKTIVGFSHTHFSGTGHSD
                     LGDILLMPTTGPLQLNPGTADAPEKGYRSLFSHSNEKASPGYYSVLLEDHDILAELTA
                     TTRTGVHRYTFNRGGDAHLILDMVHGIYNDAGKNVWTFIRVENDTLVTGYRQTSGWAR
                     TRSLHFAIAFSKPFTSYGFRDDSPARVYRGFWRRFDQRTNFPEAAGRNIRGHFDFDAE
                     PGEQITVRVAISPVSSAGAMANMIAEAPRADFDAARIGAEASWRKELGKVKAEMADEG
                     QMTSFYTSLYHTFLSPTVYTDIDGSYRGLDQNIHRAEGFTNYTTFSLWDTYRALHPLF
                     TIIQQERTADMINSMLAHYDQSVHHMLPVWSHHANENWCMIGYHSVPVLADAIVKKIG
                     GFDHARAAAACVTTARNSWYDGLDDYMKLGYVPDERTGSSVSVTLEYAYDDWCIAQIA
                     GSEDLRREFAERSQNYMNVWDHATGFMRPRKRDGSFRKDFDVLSTHGQGFIEGNAWNY
                     SLYVPHDPAELIRLHGGKKRFTEHLDSLFTMYLPDSFFAETEDITREGIIGNYVHGNE
                     PSHHVPYLYNHAGAPRKTQYRVRQIMDTKYLPAPDGLSGNDDCGQMSAWYIFSALGFY
                     PVAPGSDRYDLGSPLVKEATIALENGKKFTVTAVNQGSKNIYVKKAELNGTKLDRHYI
                     THDEIMNGGSLVFYMSK"
     gene            complement(27340..28374)
                     /locus_tag="PKK03_00440"
     CDS             complement(27340..28374)
                     /locus_tag="PKK03_00440"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HNX82884.1"
                     /translation="MKKYLILPLLAAAMASCATQELFLNVTQPAPVTIAPEIKTVGII
                     DRSTPTDQTKSLDNLDKLLSLEGTDLDSIGTREAIKGVTEELAANDRFNEVKLLNGLQ
                     FRTSSLGGLPVPLTWEQVEMICNENGTQALFALEMYDTDTRVNYSTEPTKIKTPLGSI
                     PALNHIASMETLVKTGWRIYSPSDRAILDEFIVGESIVFAGKGINPVAAVAGMVNRKE
                     AVKEVSRKAGHIYAIRLIPYRLRVTRDYFVKGTGSFKIAKRRAQLGKWDEAAELWQVE
                     TTNPKMKIAGRAHYNMAIISEINGDLDAATSWAQKAYSDYGNKHGLRYVRILENRKYN
                     EEVLKYQEQR"
     gene            complement(28600..30132)
                     /locus_tag="PKK03_00445"
     CDS             complement(28600..30132)
                     /locus_tag="PKK03_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HNX82885.1"
                     /translation="MDTLIIKGSGLTVNDVVNVARHGQKVELHPDARKKILECRAMLE
                     KKIVAHEIMYGVNTGIGEFSEIVLNDDQIKDFQKYLVYNHAAGIGDPAPIEVVRGAML
                     GRINVHAHGNSGCRLEITETYVEMLNKGVTPYACQKGSVGACGDLAPMSQIALLLMGE
                     GKAYYKGELLDGAEAMKRAGIKVPGLLARDGLAAINGSNMLTAMSAIWLVDANNWLKQ
                     AEIAAAMSLEALKANMKPYAPLLHEARGFKGAIRSANAINKCVSGGDLKEGRVKSKVQ
                     DAYSMRSTPQVIGAAHDMLGYARSQVEIELNGVGDNPIFFPDKNLQLSGANFQGSPVA
                     VPMDMAGASVTMVSVMSERRMNRLNNPALSVGLPAFLTKGAGMFSGLMLSQYTADMMI
                     VEQRILSAPASIQSIPAAADQEDFVSMGMNTAIKNNQILDNAYGILGIEYMAAAQALD
                     FRKSEYSFGTGVQKAKDVIRKHVDFLDIDRPLYADHTTMKELVKSCEILHEVEKAVGS
                     LE"
     gene            30442..31815
                     /locus_tag="PKK03_00450"
     CDS             30442..31815
                     /locus_tag="PKK03_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNX82886.1"
                     /translation="MKSRFTSTLLLVIVPAAILLTLAAFNIIRSLPQEDLKGKYQYKD
                     FEPALKCRSCHPGIYEQWSQAMMSQAYTHHWDEIEYFELAVPHAEVNPALKDPVDGCN
                     GCHTPMAYMVGKFPPPRPGEKSMANESVSCEVCHLTQRAQVDPPFNFSYFIEPGRTKY
                     SGREPEIQSPAHKIVQNDFFRTTEFCGNCHNEKNPYGIWVKSTQLEWKEGPYAKEGVR
                     CQDCHMPKGPYILALMGKERDDARLHLFHGAHDPGKVRGTIELRIEPDVRLAEPGENI
                     VFTVALFNQKTGHKFPTGSVEDRIAWLDVEATDAKGKTYHLNVDKKGFEGEEYTISGD
                     YLAYQDMAIPLKLTDFRGVQRDGIPYGNRIFRMPYFDEQGNMTIMQWNTFSQGVDYRI
                     GPRETKVEKFTFKIPYEAAPGEMKVRAVLNYQLLVKPVADYLKVPSDESEIIMVNEHS
                     TRVEILP"
     gene            31840..33042
                     /locus_tag="PKK03_00455"
     CDS             31840..33042
                     /locus_tag="PKK03_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82887.1"
                     /translation="MKTSFYARAAILTATIIMLLPYEQVSAIPAFARKYQISCQVCHS
                     PAAPTLKPFGDEFAGAGFRMTDYESPRYFIQTGDDRLSLFRELPLAIRIDGFATLNFN
                     DAGTVDFGSPFVVKILSGGELSDKLSYYFYFLMNERGHIAGLEDAFLMYSDVLNSGIN
                     LFIGQFQTSDPLFKGELRLTLEPYKIYGVKPSESSVDLKYDRGIMFDKGFSTGTTIVG
                     EIINGCGIGEAGEGYLFDKDKYKNFMLRLNQAIGNSVSIGFFGYTGKEVITDNVSDPY
                     VSEMKMYGPDLSVDIDGKLLLNVQYLWRTDSDVFDGDGEVHLTDVKTQGGFAEVIYAP
                     KGDMSKWYFTGLVNWVDSDLDQLDYQSGTIHAGYLLRRNVRLVTEYTRIFGGNPYGKL
                     SAGFVSAF"
     gene            33146..35032
                     /locus_tag="PKK03_00460"
     CDS             33146..35032
                     /locus_tag="PKK03_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNX82888.1"
                     /translation="MKKLMVIAAVALLASCTVKKEVDQYTIDQFYKNVNTWFSGFSPD
                     ETKILVSSNETGIYNVFEISLGDGSKRQVTSSTDDSYFAVGYVHGTGQILYSADKGGN
                     EINHIYLLGEDGSTVDLTPGDEVKASFNGFSPDKKVMYFSTNGRDPQYFDLYRMNVGE
                     WKPEIIWENTEGFDYSGSTRDRNILALAKSITTSENRLFLFDRVTGRTTEISEADKPG
                     SYSASGFSNDNASFYYTTDVGKEFAYLVKYDIATGGREVVYEADWDVRGCSFSRNEKY
                     RMIMVNEDARIKLKITDQSTGQDVVWPDIQGSNIEDLAISESEKYLLLGLGTSRTPGD
                     LYLYEFGGTDMKKLTNNLNPEINPANLTEAEVVRYASFDGLEIPAIYYKPLTATKKNK
                     VPAMVLVHGGPGGQAMTYYDPLIQYLVNHGYAVLDVNNRGSSGYGKSFFKMDDRNHGD
                     KDLKDCIWGKKWLQTLEYIDSENIGIIGGSYGGFMTMAAMTSAPDEFKVGVNLFGVTN
                     WLRTLKSIPPWWGSFRESLYNEMGDPFTADSVRLYNISPVFHGQNVKHPVMVLQGAND
                     PRVLQVESDEMVEAIRSAGVPVEYVIFPDEGHGFVKKENEIKAYSQIREFLDKYLKGT
                     GEES"
     gene            complement(35139..36374)
                     /gene="hydF"
                     /locus_tag="PKK03_00465"
     CDS             complement(35139..36374)
                     /gene="hydF"
                     /locus_tag="PKK03_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HNX82889.1"
                     /translation="MTAKGRDTKPHVGIFGRRNVGKSSLINALVGQDIAIVSDVAGTT
                     TDPVKKSIEVHGLGPLIFIDTAGIDDTGDLGELRIKKSMASLKLIDLAILVLSDNRFG
                     DHEARLTEEFRKLDMPYVVIHNKSDVTAVTAELREKVMSLTGREVAEYSALHPEKYNP
                     ALLKMIREAMPATALKSHGLIGDLVNPGDIVLLITPIDIEAPEGRLILPQVQVIRDIL
                     DNDCTAIVVKEREVDAFLRRTGIRPALAITDSQVFLKADASIPRDIPLTSFSIILARQ
                     KGDFAEYLRGTPKIGELKDGDRVLLLESCTHNVSCDDIGRVKIPRWLTNFTGRNLEFD
                     NVAGLDNFPRPIEEYALVIQCGGCMVTHRQLLNRLREPKKHSIPITNYGMAIAWVHGI
                     FNRATEPFTGRKAEATDLL"
     gene            complement(36379..37839)
                     /locus_tag="PKK03_00470"
     CDS             complement(36379..37839)
                     /locus_tag="PKK03_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNX82890.1"
                     /translation="MKSNKTMRREKDFLGEKELDENALYGIHSVRARDNFPDQGRFNE
                     EWYRAMAVTKQACYLAAASFFKEAAKEYDLAAINIRVITAEKLGALSAAAGECMAGKH
                     FGHFIVPAISGGAGTSINMNLNEIIANRALQILGRDPGDYDFIDPIEDANIFQSTNDV
                     VPTALRVAAMNLLTEFEESINELRKNIEELEKKYRSVLRIGYTQMQEAVPTTWGRLLG
                     SYSDALSRDWWRVSKCLERIKVVNLGGSAVGTSITVPRFYVNEVVNRLQQLTGLPVTR
                     GENLSDATSNLDPLVEIHGIIKAHAVTMEKMAGDLRILASDLHGRRSVSIPARQAGSS
                     VMPGKINPVIPEFVISCSHRVYSNDQIVSSLAAQGCLELNAYLPLTGHALLESIKTLI
                     AADHSMAENLLSGMEVHSGEAEKQVRSSPAVTTALLPFIGYKKAAALAALMKEERLSI
                     SEANKKLGYVSPEKLSDILRPENLVQGGYRLKDIEE"
     gene            complement(37829..39019)
                     /locus_tag="PKK03_00475"
     CDS             complement(37829..39019)
                     /locus_tag="PKK03_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82891.1"
                     /translation="MEKLNLFFDRIKDLSFWQRLFHWRRVRNLSFDAYEEFRALARET
                     EAVRETTGRLQNRITELSARNENLSQRVREMEVQSARKDAYIEELNGRLREHSAGIQE
                     LTKKVAGFEAIREDNVKTYNENIARLNQTKDSLEAERRRLNEEKLREVADRLEAMKKQ
                     WSEHETDVKNSIIRICDVNHVNYIEKVPFRGNPDNTIEISGEYIIFDAKSPANDDLSN
                     FPTYIKIQADNLKKYASQDNVKKSMFLVVPTNTIDKIKQLTYRLGDYDVFVITRDALE
                     PVILSLKKIEEYDLAEKLSPEDRDSICRIIGKFAHTAKRRIQVDQFFSEEFIDIVLKS
                     QKDLPEDLRKQVIEFEKSEKLNPPTEKRAKIISIDELQKKSEEIDAEAQSKKLSPPVS
                     EDEK"
     gene            complement(39136..40266)
                     /locus_tag="PKK03_00480"
     CDS             complement(39136..40266)
                     /locus_tag="PKK03_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HNX82892.1"
                     /translation="MKNVLSAFGLSMILAISACTQTADYDLKSWPADADPGKVGLLLS
                     ERYLATPHKSSGDVSSLQPPTQIVYSEVCTWLGSIWFAEATGNSELLTKLQSRFEPLF
                     DTITNLLPPPNHVDNNVFGSVPLEFYLINGEKKYLDLGLYYADTQWTLPEDAGPEEKA
                     YADLGYTWQTRIWIDDMFMVTAVQSQAYRATGDIKYIDRAAGEMILYLDTIQLGNGLF
                     YHAPYAHFTWGRGNGWMAAGMSEILRAMPVDHPDRPRIMEGYKKMMATLLIHQAEDGM
                     WRQVIDDPEFWKETSSTAMFTYAMITGVKNGWLDEKTYGAAARKAWLSLVKYIDAEGN
                     VTEVCEGTGTSDDRSHYMNRKRNTGDFHGQAPVLWCAAALLR"
     gene            complement(40277..41149)
                     /locus_tag="PKK03_00485"
     CDS             complement(40277..41149)
                     /locus_tag="PKK03_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNX82893.1"
                     /translation="MDRYTISDLEKLTGIMAGTIRIWERRYHIIKPHRTDTNRRWYDN
                     DDLKRILNISILYRKGVKISKIATFSGDEIEEKVKLLTRESSDTETQLDTLIVAMSDL
                     DEYRVNDILMRSIINKGFEETFTEVVFPFLRRVGIMWHTGSVDIGSEHFVSNIFRKRL
                     ISAIDTLSPPERPDRKKFIMFLPESELHEMGLLFFTYIIRKAGHSVLYLGQSTPFNGL
                     KDLVERWHPDFLVTGALTSLPFGKPEDYLKRISSAFRDQTILVAGILALLAGKMSLDN
                     VIPFDSVNSLKKYL"
     gene            complement(41269..42183)
                     /locus_tag="PKK03_00490"
     CDS             complement(41269..42183)
                     /locus_tag="PKK03_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNX82894.1"
                     /translation="MKKQTLIYGMIALMGIFILTGCEKAEEEMKPVSDMTIAEYAGSD
                     ANFSILVQALTKADLVNILNGTGNFTVFAPTNAAFTSLFNQLGISGIDALTKETLTPI
                     LLYHVLGTEAKSSMISSGYYKTLSPAQGSNLSLKVDVASGVKLNKTANVSTADVDVKN
                     GVIHVIDKVLLPPTVVDQALNNDSFSILVQAVVKANLVETLNGSGPFTIFAPTNAAFE
                     SLFTTLGISGVADLTAEQLVPILTYHVVSGNVLSTQLQAGNVETLNGAISVALSPVPT
                     INGSTKIVATDVQASNGVIHVIDQVLLP"
     gene            complement(42423..43385)
                     /locus_tag="PKK03_00495"
     CDS             complement(42423..43385)
                     /locus_tag="PKK03_00495"
                     /inference="COORDINATES: protein motif:HMM:NF022779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HNX82895.1"
                     /translation="MSRYGTFREFYEKELRPDLESIDLRRRATNKKALTALLITAAAV
                     LAELMLIPGNTAFPKPAPIIITAFAGLVATGIVSKNYRKEYKEKIIARIAGYADEGLT
                     YQPDACITMDDFIRSRIFTLSCDKYSGEDYFRGRIGKTEIEFSEVNARHRSTSGAGSH
                     QRDEYTTIFKGIFIIADFNKNFKTHTVVLPDTAEKIFGKFGQSLQSVSFGRGELIRLE
                     DPRFEKEFCVYGDDQVESRYILTPSLMERILAFREKWNTKVHLSFLDSKVCIAISMNR
                     NLFELRPFKPAADYDFLEETLRFLTLLTEVVDDLNLNTRIWTKE"
     gene            complement(43400..43963)
                     /locus_tag="PKK03_00500"
     CDS             complement(43400..43963)
                     /locus_tag="PKK03_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNX82896.1"
                     /translation="MIYVLIAAAFILLLSALLIFNSLIRRKNDVENSFASIDVMLRKR
                     YDLIPKIVETVKAYMNYERELLTEITELRIKALPQTLTAEERVQVENRLSARLTDLLV
                     AVENYPDLKASTNFLQLQGTLNEVEEQLAASRRAFNAAVTIYNNSIEIFPSNIIASMM
                     QYKRRSFFEIPAAKREEVSSKPPVNMA"
     gene            complement(44028..44720)
                     /locus_tag="PKK03_00505"
     CDS             complement(44028..44720)
                     /locus_tag="PKK03_00505"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNX82897.1"
                     /translation="MKSLLIILTLVIFLPSDQEEVRNTRARSQMVKSQIVARGISDPA
                     TLRAMERVPRHLFVPVALAGSAYSDQPLPIGYNQTISQPFIVAYMTELSKPAKGKKAL
                     EIGTGSGYQAAVLAEIVDTVYTVEIVPELAREAASRLKVLGYGNIVARYGDGYKGWPE
                     HSPFDIILVTAAADHIPGPLKEQLAEGGRLVMPVGNPATVQELIVLTKRKGRITEQRL
                     EPVRFVPLKRLK"
     gene            44857..45549
                     /locus_tag="PKK03_00510"
     CDS             44857..45549
                     /locus_tag="PKK03_00510"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HNX82898.1"
                     /translation="MKKLVILTGAGMSAESGIKTFRDSGGLWEQYKVEEVASIEGWYR
                     NPDLVQKFYNDRRRQLEKAVPNAGHTGLKELEKLFDVHIITQNVDNLHERAGSKSVLH
                     LHGLLTEARSSKNPDSVIDIGYREILPGEKAADGSSLRPNIVWFGEAVPAMEAAADIA
                     SQAELFAVIGTSLNVYPAAGLIHYVPASIPVFLIDPKPVTVYTNHKVDYISEGAGKGV
                     AILAGKLMVFAA"
     gene            45722..47227
                     /locus_tag="PKK03_00515"
     CDS             45722..47227
                     /locus_tag="PKK03_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNX82899.1"
                     /translation="MQAQQNKVVDRIIELGQTDNRTMHHLDVLSNRFGGRLVGSDAYE
                     NAADWCASQFRSWGMEVIMDEAGTVPVGFNRGPWFGRMLSDNGMTLHFATPSYTAGTK
                     GVQRGHVLLEPKSKDEFERMKGVLKGAWVLIGGKNDGWPIDWSPRGDSIRAAAMVYND
                     SISRLNREIMRENMMNRENPPKEPHKLREVPALFYREMCEAGILGVIQSSEVPIRALY
                     DRKNLDRMTFDNLPTVCDIKLDEHQYDLIAQMARERRYFLLEFDIRNHFKPGPVKYYS
                     VIGVIKGKKYPNEYVMMGGHLDAFDVATGGVDDGSGASPTMEAARLIMAAGGKPDRTI
                     LACLWAAEEFGLVGSKHWVETNKDKLPKISNYFNRDGGPMPNTGITVTPAMYDDFEKI
                     CKPLNSINPDFPFTLKKRTDQGRPRPTSAGGSDHAYFAMNGVPTLNFDGPDVKGYNFN
                     YGEIWHTERDTYDKSIPEYQEHTSVVTAVVVYGLANLDHLLSREGLYREEK"
     gene            47254..49197
                     /locus_tag="PKK03_00520"
     CDS             47254..49197
                     /locus_tag="PKK03_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82900.1"
                     /translation="MKHGLAFAAVALLAAVIVAFGQARKVDQSVYVPADPQVVQDQDE
                     MTWDDYRPIPGMNWADPSLKPEREFRMALVAVDFSDQPFVMTLPKGSDPFGNPQIDPV
                     DRSEIPQFYTDFWLKPSAVNHGQTINGYWMEQSRGKFGITQLEAFGPYRMPKPLWAYG
                     LSEWGQNNATPDGSSVRNRMEHDIDSLWHADKGDIKGNYDAILRVYAGYDETGVWQEF
                     GEMKFNSRDEIPAEWGNPNPDKPRWAPTRYVEWTSWKAGQMQWGLSSIRQGENSGTIT
                     HELGHFAFSIGDNNNNPYVKPYRRAGSGPWDMMDRGSFNGPGGPHMRWVVPPVAGAAM
                     PAGLMLRNRLVNRFITEHDLLTVSREGLASSGPVVARITARAVKPLPGEYAGIMVRLD
                     GAEPHDRTPVTDPASDPLSPGRPRFNYYSLEVVQRIGYDSFCPDNGVLLAMNTDGEGG
                     NGGLNGFNCFNWVIDAHPEDINMVDFVKPDGEKVMRTVADYRQLNDALFHAGLNSGSK
                     FEYVDTHNRLHFYVIDIHRNEQGILSYTAGVRSLDSDVVKEPATVSAGKVAQKIAGEG
                     IVEFEVSGDDLCRLHAGVTGDGWEVKLFNEVVAPGDGRTVKIPVYVRAKQGCSRRAEI
                     TLSAVSESDSKYRSAASRKVILQ"
     gene            49286..50281
                     /locus_tag="PKK03_00525"
     CDS             49286..50281
                     /locus_tag="PKK03_00525"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX82901.1"
                     /translation="MNCLLTGSNGFIGRRLVEELSAGGNKVKCLVRSPEKFRELSSLP
                     GVTPIVGDLDAVDVLEEASACCDTVFHLAAYAKPWSKDRSLPYRINVAGTENVLRAAR
                     KAGVRRFVFTSSAAVIGPSPGTEPIDESFLRTVPWFNEYEETKAAAEELVRSYNRDGM
                     ECVIVNPTRVYGPGPINESNSVTKMISLYARGRWRIIPGDGKCVGNYVMVDDVVRGHI
                     LAAQKGRPGEQYILGGENLTFDTFFDTLATLTGRRLWLVKMPVSLMSAAAGVMEWQAS
                     FTGIPPLITPSWIKKYLNHWSLSSRKATEDMGYQPRPFAEGAKITMEWLQSGFER"
     gene            complement(50331..51944)
                     /locus_tag="PKK03_00530"
     CDS             complement(50331..51944)
                     /locus_tag="PKK03_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX82902.1"
                     /translation="MKKVISPFILAALSCTMAGQQAELLKDINAGSESSFIIYMTQLP
                     DKVVFKATNHRNTNNELWVTDGTTDGTFLLKEINPSTTTGSSIGRMVVYKDEAYFSAD
                     DGTHGSELWKTNGTTEGTIMVKDIAPGSTGSKPGHLIEWNNVLYFSAYHPDTGTELWK
                     TEGTTETTLLVKDLTEGAASTQPGYITTGSCGLYFNGLSSLKLYFSDGTPGGTILISD
                     QIRVGQLDEAYFTEFKDEMYFRAKDQISGTGSQLWKVSGNGVIRVTDIVDGTTQFDPR
                     HITVVGNKMFFEAEKTPYGRELWVYDDNGIHMVRDINPDGHGNLSGGFFTGFKNKLVF
                     SAGVTSTGVELWVSDGTESGTKIIKDIWPGIMGSGVVSPVIIGDTLYFSANDGTDGEI
                     WRLLSPDGQPEKFTNVANGGAWGPEFRRINKTFVFNGAIPSTGDELWKMTLSGSTSAG
                     SINAPALALNVYPNPASGNFTVEIPECINLPCLMSICSVSGITVKIMEVTSRSTSVEQ
                     KSDVKLRPGTYIVKCGNGHKTAFTKLVIR"
     gene            complement(51981..53669)
                     /locus_tag="PKK03_00535"
     CDS             complement(51981..53669)
                     /locus_tag="PKK03_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82903.1"
                     /translation="MTTLTYSKRVLTTSLLILIVGFQAGAQRRCEECARHGMLVEFLN
                     PHNQDYEQRLKEWNACMTEQLGDREFREEDSLNPELTDAIDKCKTLDPPEKWYCYPTM
                     MSVYMGERFTNPCFHFLSPIFYAPETDKPPEYIFKGSYEANLKGGRIIEYEEDYKKPV
                     IAKMELRLYYNGNTPELIQEWSSENTINDPRPLFNKLKTPKSLNPIFEEFEKKPVSID
                     VEVPEQEEICEQGKGIIILSGFKDVGGRPSREFNRIVVSIYRGKILNGADSDYGPDYK
                     VFNVGEGEVRVEYQPPEDKDDGYEWLRVYNSCDILPKDKHPLSATAPDELIVDQHFPI
                     FCGFYKGTITVSKRWDYTKDHGKSSTRYSGSQTVSFTGIFKPIPQMEGMEGQPITIFG
                     PDKVTGTWSHKEDRYCSGDCDCTGLVDQEFGSGDFPRESIKGLILITNLFPTDNKVVA
                     DQLGQFGLENWYDIATPTENVPTETRSRHYVKDVGCVWDNSTSTDNLIGADARFKVKD
                     INHLHGDVSWSSSKEATGVRITDMTEAIYEQIPFDPEKDGTDYHYIVRWDLKAL"
     gene            complement(53673..54197)
                     /locus_tag="PKK03_00540"
     CDS             complement(53673..54197)
                     /locus_tag="PKK03_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82904.1"
                     /translation="MKKILSNKIMLSLTLLFTVSCFHSCDWFTTDPDYVGTWQYTEKG
                     YAGEVAINITHTLKLAEGTFEEVVIYRRDNSSAVMSLLGLKGNITVDGDEVLFRLTAL
                     GDCLEDTNDNCTESPEWFPKGSATYNAYLSLGMSESFEGTFEADDDYLWLVRDINNDT
                     DTDDDWEDVEFERI"
     gene            complement(54256..56253)
                     /locus_tag="PKK03_00545"
     CDS             complement(54256..56253)
                     /locus_tag="PKK03_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82905.1"
                     /translation="MCTNCRHIAALLLVQAICAASLSYASEPGSAHAPGTKQWRQYDI
                     NQDGQFDRSDIDGFIDRGGLNIETDLNGDDRKDVEDALALFLELSVMDRSCDRIVNDE
                     DFEPVDPVSVPEAPGISTVRKIVAECVMHAIPNLPPDIEQQAFRSVPQGKILSFDERV
                     FVYQLAGMSGLAQQNLDAASWGYGRAFQTNERSAGSIGSLAFCLAVSDRDAEALLLLA
                     YARSLFRESAPTATSLGWVFARHGQNEEALKYFREAVFYTPGIAQYHMNLGIILLRMG
                     MKREAFEEFREASELDPADAKKLLFWYTTKPPHEPPVKKSLDPEEFKQQIEIERKELQ
                     EEGFTEDEMVTPWDQMSPCEQARTIPELLERRYDEQMQNIAQSYSDALSKRVEGVIMD
                     FWPRWKNMPEDWNRYISGIPVVMRTSTRLTFNSMMEAGHRRASLTRQMGSELLGYSSF
                     FMECALQQARISANEQINSWRDMPLTAESMAELRAEAYQEALEEAINYCYKSQVDQAY
                     RWLTAEASPYGLPKSNIDAIDPQDFLLLFMVIPLECFNIKDYCPDGEGGDTRKPEFPV
                     EHTVSLDLFIVSIEWNTDNNEWEFNIGAGIEVGVTWKPETGFGFQLGAGVDFMGMEEA
                     VYVRIDEGKLTIETEEGFGVGTGFLSAGFEVTTIQATAELY"
     gene            complement(56329..57633)
                     /locus_tag="PKK03_00550"
     CDS             complement(56329..57633)
                     /locus_tag="PKK03_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX82906.1"
                     /translation="MKHSMLFTLVASLLFLLPQEASPQKVRVNVKEKVVEETDHRANR
                     KTDEVIDAGFDKIEEGIGNIFKKKEKKAKEDSSQKEAGEESADPGNTSGRNPTEAKES
                     GQPQLTFKWNRYDFVPGEKIIFEDNQEGEENGEFPSRWDLAGGGNVENASFGDQNVIH
                     FREAGSCIIPFFRETAKDNLPDLFTVEFDCWFEPDEYCQYYVDFWDHKNQSESTISID
                     PLIINANHAGINDVGEGYYPGYEEKGEITSGYWRHVAISFNTRALKVYLDDARVVNVP
                     NLGINPEGLTICCDPMNTVGSQGNNRFIKNIRIAEGAVRLYDKLMQDGKIVASGIRFD
                     VNKATIKPESMGVINEIAKLMKDHPEISFSVEGHTDSDGEEAFNQDLSERRAKAVADQ
                     LAKMGINNSRMTSRGWGESKPSGPNNTSEGKAANRRVEFVKR"
     gene            complement(57827..58579)
                     /locus_tag="PKK03_00555"
     CDS             complement(57827..58579)
                     /locus_tag="PKK03_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNX82907.1"
                     /translation="MRAVIVDDEAPVRSIIATLLKESFPDITVSAATGSVAEGIAAVT
                     EHQPDIVFLDIELPDGLGFELLRQVSPVRFRTIFITGHQEYALDAIKVSALDYILKPV
                     DEDELRAAILKAREIINHEEEQLKLLALRENLQGKKVLKRIILRTAEALQLISVNDII
                     RAEAESNYTHFHLAGGKHIIVSRTIKEYEAMLSGTSLIRVHQSHIVNLNYIDKFYRHD
                     GGYLQLKDGTIIPVSPVLKQKVLQSLNDHLYE"
     gene            complement(58593..60707)
                     /locus_tag="PKK03_00560"
     CDS             complement(58593..60707)
                     /locus_tag="PKK03_00560"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX82908.1"
                     /translation="MVLLLVIPAGHLFSQELADTAAITKTAEEAYTMARRNPDTAIMI
                     SHQALTASRKLNYIKGMADASLALGMAYLAKYNPGDSALYYNSRALELYEASGNITGK
                     ARACYGLSYVYSFRSDHASSEHYINLALKYFEESKDHRGMVNSLNVLAYLARQNQDLE
                     AAKGYLEKAIAIASQVKDTLPLADATNNLGNLYKEMALFSQAIDAYFEALRLWEAKRD
                     TSGMSIAYGSIGLAYYYQKNYDKALEFSRKHLMLSEKRKDLWEVSKICNTIAQIYNSR
                     EAFDSALVYQRKSLVLNRLMHIPVGEASACHNMASTLLLLSQPDSALNYMKMAMDITS
                     SSDLPVPPEFYNTLGNIWQTLGNYDRAYSNSLTAYRLGKEKELPLVVSDATLLLSDIC
                     SKRGMEDKAYRYLKEHMQLSDSISNDEFLKQVTRMEIQYDFDKKEKAAEFERMQERFL
                     SETKIRQQRILLSGLVLLLAIIAILALLYIRHARLKARYTQVDLEQRLLRAQMNPHFI
                     FNSLCAIQDLILKDKPQKANAFLTKIAQLMRSILENTREEYIALDKEIETLKLYMDVQ
                     QLRFENGFSYGIEIDEAIDPENVSVPPMLAQPCVENSIEHGLLPGTKDGRISVRYSLR
                     NGLMMLEVADNGIGREKAAEHKPLMKKESISTRLTEKRLEHFRKILKEKQISYEIIDL
                     YEDGNAAGTRVVMMLPWKKIYA"
     gene            complement(60812..61531)
                     /locus_tag="PKK03_00565"
     CDS             complement(60812..61531)
                     /locus_tag="PKK03_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HNX82909.1"
                     /translation="MTALTNDELIRYQRQLTVAGIGEKGQELLRKASVVVIGAGGLGS
                     PVLMYLAAAGVGRIGIVDSDEISTGNLQRQILYETGDVTRPKAETAAEKLRRINPHTE
                     ALAYSVRFTEENAGGILAGYDIAVDCCDNYSTRYILCDATLKAGIPMVYGAVREFMGQ
                     ASVFNYMGGPSYRDLYPEEMHLKTEDRSGPPGVMGPLPGIIGAVQACEVIKIITGAGD
                     ILSGRLLQADMLDQRIDIISL"
     gene            complement(61555..62166)
                     /locus_tag="PKK03_00570"
     CDS             complement(61555..62166)
                     /locus_tag="PKK03_00570"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNX82910.1"
                     /translation="MENEILRIARRVQAIAQSGIHFAASEFDRERYEELRGLSVELAG
                     TVCDTRPSKIHDLFTNETGFQTPKVDIRSVVLKEGKLLMVREKIDGRYSMPGGFADIN
                     YSPSEVAVKEVREETGLNVRFNRVLAIADTDRHGFPPLPYHFYKIVVLCDLVDGVLRD
                     SIETSEAGFFDFDNLPELSVERNTPAFLEMIRKQLERAETYSD"
     gene            62590..63846
                     /locus_tag="PKK03_00575"
     CDS             62590..63846
                     /locus_tag="PKK03_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNX82911.1"
                     /translation="MKNTFLSLAIIVVAGTTGQMTSCEDQTEKSLPADPVEINLSLKQ
                     REVVASANRFGFDLFRPVVAGEETGTNIMISPFSVTSALSMTLNGASGETFNAVRSAL
                     RYDGQSLEEVNETYRRLVTEMVPVDERVVMEIANSVWAENNFRVKKEFMDALKEWYLA
                     EARNFDVTDPRSVDIINGWIEEKTHDRIQNMLSSLDRDLVMLLVNAVYFNGKWKHQFD
                     INNTAERPFFVTPGNPVKVPVMYQKQKFAMSRQEKVTLVELPYGQGNYSMVVALPDEE
                     IAPSEIVTGLDAGKWEEWMESLSYGPTEVELYMPKFKYEYKRKLNDDLISLGMDPAFA
                     PGIADFSRISDEEIFISFVLHQTFIENKEEGTEAAAATVIGFTRTSLPPEPEVIDINR
                     PFLYFIRETTTGTIVFMGLVADPTGE"
     gene            complement(63900..64613)
                     /locus_tag="PKK03_00580"
     CDS             complement(63900..64613)
                     /locus_tag="PKK03_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007411257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX82912.1"
                     /translation="MIKVTNLVKSYKRKTVLNISELTIHDGEIFGLVGNNGAGKTTLL
                     RLMLDLISPDRGSVTSEIHVVARSEKWKDYTASYLDESFLINYLTPEEFFGFIAEEYG
                     LAPELTEARLAGFSEFFNGEVLGQKKKFIRDFSRGNRQKIGIASALVTEPRVLILDEP
                     FNGLDPSSQIVLKRILSDFNRANGTLMVISSHDLNHITEICTRIAIIEKGVIIRDIQN
                     DGNAMAELASYFSLRKESM"
     gene            complement(64625..66115)
                     /locus_tag="PKK03_00585"
     CDS             complement(64625..66115)
                     /locus_tag="PKK03_00585"
                     /inference="COORDINATES: protein motif:HMM:NF039827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5687 family protein"
                     /protein_id="HNX82913.1"
                     /translation="MKEAGTYLYSHIWKSFIRNERWRRNLITRLLYALVGLNFIFIFL
                     TLGLDVSSTIAGEGREASGKFHEWIVWYLLADYLVRCIIQPVPSLDVVPYLRFRIRRS
                     KLAGNIIIRSLFSLFNFLPLFLILPFVIKVTAPFEGIQAALLFLGGSFLLVIMNNILA
                     LMTGMLTRINPLNWLIPIGIATAFFIFDRMGGSVGDISRSLGMALTEGRPLAYFIPAV
                     LITAAVILINRLLHQYLRVDRGSSVSSYRTHRNAFTGRLARFGDTGRYMSLEMAMILR
                     NKRPRNAMFFVLFFIVYAFIFFLAYDEQERYINMLFTTMFLGLGAMSYGQLLFSWEST
                     YFDGVMARKNDFLNYVRAKYYLQAILTLITFIPIAVLSYISGRISVLLTTALMIYLLG
                     PNSCLTMLLATLNDGKVDLEASTFMNYQGVKGSQFIMTFFFVIIPIAICELTSLVAGE
                     TGGIIVLATLGILFVIFHKWWLKNIIVATFMKRKYKLLEGYRKLSA"
     gene            66241..67140
                     /locus_tag="PKK03_00590"
     CDS             66241..67140
                     /locus_tag="PKK03_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82914.1"
                     /translation="MNLTYIDFLVSAVLATIMFGVGLSINFSDLQKIYRSPKAFILGL
                     VSQMILLPLIVFLIIGFTDLHPTLKVGFIILAACPGGTTSGFVTVLFRGNVALSVSLI
                     AINSLLTLATIPLLVNLALTVFMGKHSPFELPLVASFLQIFFITLLPAITGVLLRYFR
                     EKIADRIQKPVRTVMIILFAGVYIIIAFAKESSGGSGITTEELWKILPYAVLINFAGF
                     AAGMAMGLSGKSGLRTSWTIGVEVALQNTTLALLVAGTLIQSNEMVKPALVYALFSFW
                     TALLYGVVVKWYDGSKLFGEFRK"
     gene            complement(67203..69536)
                     /locus_tag="PKK03_00595"
     CDS             complement(67203..69536)
                     /locus_tag="PKK03_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 protein"
                     /protein_id="HNX82915.1"
                     /translation="MKPPVLICALIAFMLLAACSNDQETAPSLVPQPVEMVLSHGQFR
                     LASGTRISWSGSQEALAAAGQLASELRPATGYELAVNEGIDGDISLLIDVSLEWRSEE
                     YRLKVEKKKVTITAGSGAGLFRGIQTLRQLLPPQAFSHSQEEEVIWTIPCVSITDYPR
                     FGWRGMHLDVSRHFFDKEFVKKYIDILAMHKLNVFHWHLVDDQGWRIEIKKYPGLTEV
                     GAWRVDREDKPWDSRQPQQPGEKATYGGFYTQDDIREIVVYAAERHITVVPEIEMPAH
                     VGSALAACPEYSCTGGPFTVLPGGVWPITDIYCAGKDETFSFIEDVLTEVIDLFPSEY
                     IHIGGDEADKTEWKKCPDCQARIRKEGLKDEHELQSYFVKRIEAFLTSRGRKLIGWDE
                     ILQDGLAPEATVMSWQGFEGGIAAARSGHRAVMAPVSHCYLNVYQGDPATEPESFRGL
                     LTLKKVYSFEPVPPELTADEAKMIIGAQGCLWTEYISDGATAEYMILPRLTALSEVVW
                     SPAGSRDWEGFNHRLPKMMERFDAAGINYSRGSFHVDMSASNDEATAGIILEMTSEQP
                     SPEIRYTTDGSDPVAGSPVYDKPLKMTEKAKVKAAIFREGKMIGGISSKNISINRATG
                     MKVQYNIPFSDRYRGHGDRTLVNGINGSGDFNDGHWQGFEGTDMDVVIDLGKETGITS
                     VSSSFMAAVGSWIFLPRSVEYSCSDDGSNYTPLGTVITEIDPADQGNRTETYFSSFPV
                     VTARYVRVVARGEITCPPWHAGAGSKAWLFCDEIIVE"
     gene            complement(69545..70882)
                     /locus_tag="PKK03_00600"
     CDS             complement(69545..70882)
                     /locus_tag="PKK03_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HNX82916.1"
                     /translation="MRRTILTILLSLVIISLGAHPWKPRHFVIIDSDGGIDDLKAICM
                     LMASPDVRILAITASDGFHKAPVAYEKLRDLADGLYHEGLPVAGPGDAVALIEKMLAS
                     ETTPVKFLAMGSLKTAAVAMEQSPSFAGKVKQIYWSNSGLPGKDGSNYRSDPASAEKV
                     LAGKIPVTVTGGGGEGFYDSGLLGNISMIRSPYAFRVSDLINSMPSHSFAYTAYDEMV
                     PLLLHFPELFSQEGSDKRITYRTPSDINGLRTAALKMLRGETVERMQTIKRMPADTSF
                     YMPDIQPFVSGIRNKYGEDEWTSGVIANELHRHLGVFAIIGVKMGIRAREYFCTGVDE
                     MEVTTHAGSTPPLSCMNDGIQVSTGATPGHGLLTVSPKQPYFPAADFTHKDRTVRITL
                     KNELAERVTAELKEINFIYGLDSDIYWELVRQNSIKYWLQFDRHEIFDIEVIK"
     gene            71084..72523
                     /locus_tag="PKK03_00605"
     CDS             71084..72523
                     /locus_tag="PKK03_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNX82917.1"
                     /translation="MKRKTRILFFIGLVAGMVILAGFGKAVEYTSTNDFCAVCHVHPQ
                     ATTSWKQGIHYATQSGYRTGCAECHLPPKGEGYLVEKAKTGMRDLWSHWTKDSADFDW
                     EAASTLERASKHVYNSSCIHCHENLFPAKLTRDGSEAHLYYKQMTEKGEDLQCISCHL
                     NAGHYIEGYIHGSNTGFGASVARADTLYQYPAKVTGFKDFTEQVPGSAVTFRMIAVPG
                     GEFTMGSPEGEPLRGEDEAERMVKVDSIFIAEVEVTWDEYMEFYRQTASEGRSTDTEG
                     ARAKAAGVDAISGATPPYGKPDQNWGMGQRPAISMSWHAAETYCRWLSKVTGKNYRLP
                     TEAEWEYVCRAGTTTPYFFPGDPAKFEKKGLFGGKPDTSVISTFVIYHGNSPSKTQEP
                     SAVKPNPWGIRNMSGNVAEFCSDIYEGTSEHVIRGGSFRDGAAGVRSAARDHTRTDEW
                     LKTDPQMPKSIWWYSDCFHVGFRVVMDWK"
BASE COUNT        16905 a        18223 c        18677 g        18950 t
ORIGIN      
        1 agcagatatg aataagaaga ttttaatatt cggaattata ctttcagcgc tgatgatctc
       61 atcgtgcgtg aaggacctgg atgtcacccc gaaagacccg acaaagatcc ttgccggtaa
      121 ccttggcgac aatcctgaat acatgaaaat ggcccttgga aagctctatg ccagcttcat
      181 aatctccggg cagggtgcca acgggggtga cgacattgcc gcttctgacg gtaacttctt
      241 cacaaccatg agggctctct ggaacctcca ggagataacc actgacgagg ccatttgtgc
      301 atggggtgat gttggcatag ccgacctgaa cacccagaca tggagcccca gcaacccgtt
      361 ccttacagcg ctctatcaac gtctggcgct cagcatcacc tatgccaatg acttcatcaa
      421 caatgcacag gagtacggtg gtgcaaatgc tgacaggtat gtggctgagg caagattcct
      481 cagggctctt gcatattact gggctattga cctcttcggg cagttcccct tcctgacaga
      541 agctgacggt gtaggtaagt tcttcccgca aattggtacg aggggtgaca tattcaatta
      601 tgtcgagtct gaactgcttg acattgagaa caagcttggt gcaccaaagt cttcatatcc
      661 tcaggcagac caggcatctg catggatgct tctcgcaagg ctctacctga atgctgaggt
      721 ttacacaggc acccccaggt gggatgactg cagggagtac tgtgacaagg tcatcgccag
      781 caacgtctac tcgctggcaa ccaactacag gcagaacttc agcgctgaca acgacggccg
      841 ccacaacccg gagatgatct ttgcatggga gcaggacgga atacacgtgc agggttatgt
      901 aggcactacc tttatcattg aatcatcgag tgatgccaca tatttaaagg ctgaagatta
      961 tcacggcctt acctccaaca ccaactggaa cggcaaccgt gcaaggattc agttcctggc
     1021 aaaaatggtt gatacactcg aagtttatga taaccagccg attccggcca gcgatccgac
     1081 actttcactc agccctgaca agagggtctt cctgaagatc aaaaagtcaa ggaacattcc
     1141 aagcccgtca gccagcggcg actatggcat tggggtctac aaattcactg cacgcaacgc
     1201 agatggcact ttaccttcca actataatgc agcctatgca tgcaccgatt tcccggtatt
     1261 ccgcctggca gatgcatacc tgatgagagc tgaagctctt tacaggatgg gtggtgaggc
     1321 caacattgcc gcagctgttg ctgacatcaa caaggtcagg gaacgcgcct acggtaacac
     1381 cagcggtaat atcaccgttg ctgatcttga cctcgacttc atcattgacg agcgtggacg
     1441 cgaattctac tatgaggctc agagacgcac tgacctggta cgcttcggta aattcaccgg
     1501 tggcgattat ctgtggcagt ggaagggtgg tgcatacaca ggcgccagca cttcggagca
     1561 cctcaatctg ttcccgatcc ccggtgatga actttcatca aacccgaatt acaacggagt
     1621 gaataacccg ggttattaat ccttaatgca aagaaaaatg aagaagaata tcttaatata
     1681 tctgacattc atcggattgg cagcattctt cacctcctgt gaaaaggacg gtgagaaagt
     1741 gatcatgctt tcagatccga cagctccggt gctcaccacg gttcctgacc ttacgtttca
     1801 aagagccaac ggaatgaatg tgcttgaatt tgtcggcaca cctgttgacc ctggcttcca
     1861 ggcctctgca acctattatc ttgaggcatg tgcttcagga acgtctttcg ctgaccccgt
     1921 aaccctctgg acagggaacc agtgcaagtc aatgaaggcc accgtaagtg acctgaatgg
     1981 tatccttatc aagaagtttc ccgcagatgc atcctctgct cttgacctga gactcagggc
     2041 tgtactggtg gttgacgccg gtaccggagc tcccggaaca ggtaccaata ccttcactta
     2101 catctccgat ttgaaaagcg caactgcagc attgtacggt ctgccgcgcc ttaacctcat
     2161 tggttcgggt atgacccaga agatcgagtc agccctcggt gacggcaaat acaccggcct
     2221 tgttaagttc gatatcacaa agccgtttac cctgaatgat ccggatgccg gaaccaatta
     2281 tggtgccaca ggcactaccc tggcagtaaa cggtgcagcc attgttccta ccgctaacgg
     2341 atggcacata ctgaccgtag atgttaatgc cctgacctat gacatctcgg aatacaggat
     2401 tggcctggta ggttcagcta ccccgaacgg ttgggattct ccggaccagg caatggaata
     2461 taatcctgca accggcacat ggtcaattac aataactctc gcggatggtg agatcaagtt
     2521 caggtttaat aatgcatggg catggaacct cggttaccgt ccgggtggta aagatcccgc
     2581 tgacctgttc cataacggtg ataacattgc ggtcactgca ggtaattaca ccatcaccct
     2641 gacgattacc cagcccaacg gtcctgacga agccggttca tgcattattg tgaagaataa
     2701 ctgattcagg gacattataa taaacaagat ttatgaaaat gaaatcaatt ttgaagataa
     2761 tgatgctccc tgtagcagcg gtacttatcc ttgccggttg tactcaggag acatctgaaa
     2821 taagacttga ccccaagctt ggaactacca aggtgttcaa tatcacttca aattcagcaa
     2881 cggtaacagg atttgtcgtg gctgaaggtg agggattcac cacgagaggc gtttgctatg
     2941 acaagcagcc tgctcctacc acttcaaagt cgacggctcc gtttgccggt cctgcgatca
     3001 atgctgcgta taacgtgaca ctgacagacc tggattttgc acagacctat tttgcacgtg
     3061 catttgccac tggtcccgcc ggcactgtct atggagagga actgacattc accactctgc
     3121 ctgtcgtggc aaccgtcacc acagttgatg caactgatgt tgagggtaca accgcagtct
     3181 caggcggcag cgtcactgct gacggagggt ctgctgtcac tgcccgcggt gtctgcttcg
     3241 gaacgacaac caaaccgaca attgacgccc ccaacggcaa gactgttgat ggtgcagggc
     3301 tgggatcatt tgtaagcaat ctttcgggtc ttaagggact gaccaagtac tatgcacgcg
     3361 cttatgccac caacagcgca ggcacagcct acggaaacga gataaccttc accacgctgg
     3421 tgagcgttcg cgaatggtac gttccgggtg attatgtggc tgccagctat ccgggctcag
     3481 gcctggccga ctgggcacct gacaagtcac ctatggtcaa gagcaatgag gctaatccaa
     3541 acaatgttga agggtatgtc tatatggcta acgccagtaa caatttcaag tttgcctcac
     3601 agcccaactg ggacggaccg aattacggtg caagcggaac ggcaggagta cttagctcgg
     3661 atccgaacgc aggaaacaac aacctgcctc agggattcta caagttgaat gtcaatgccg
     3721 gtgttgaccc gatgacctac acggcagttg caatgacctg gggtgtgata ggtgatatca
     3781 acggatggag cgcccaggtg ccactgacct atgatccggc attacacgtc tggaaaggtg
     3841 ccatgcatat gcctgtagga ggatggaagt tcagggccaa tgacaactgg gattacaact
     3901 atggaaaagg caccggcagc aatgacctgg tagcaggtag ccaagacaac ctcagcatta
     3961 cagttgctga tgactatgcc ataacccttg atctcagcca gccgctggct tacacatacc
     4021 gtgctgacag gtggggtata gtaggaagtg ctacaggtag ctgggacgta gatcagaaca
     4081 tgtcatggga tgccgtaaat aaagttttca ttgccactat tgaccttact gcaggtgaaa
     4141 ttaagttcag ggcaaatgat gactggggct ataacttcgg tggaagtctc actgatctca
     4201 gctctgacaa tgcacctaat atcccgattg ccgaagctgg taactataaa attactcttg
     4261 acccgtgggc aagagtggca acagtgacca agaactaagc ggaatagttc cccatgttac
     4321 ggccgcggcc gtagcgttca ttgaaatcaa aaacctcttc tgtgacagga agaggttttt
     4381 gattatattt agcaagattc acactttcaa tcagaaacaa tgaaaatcaa gattcctgta
     4441 ctggtactga tttttgcagg gtggagcctg gccgtgtcat cacaggtgat taccacagat
     4501 ccggtcttcg cccttgcctc agcgccggtg gtgattacgt tccacgctga caggggtgac
     4561 atgggaatga aggattacac cggagacgat gtatacgctc atacaggcgt tatcaccaac
     4621 ctgagctcag gccagtctga ctggaagtat gttgtctcag gaacctggag caccacggtg
     4681 ccgccgcctg acaaagtaaa gctgacgcgt gttgacgcca acacatatac acttaccatt
     4741 tcgccttcaa taagggcatt ttacggcgtc ccggaatcgg agacgattca gaaaatggct
     4801 tttgtgttcc gcaacggcaa cgcctcgcat accggccgtg acaacaatgg tgcggatatt
     4861 ttctacaacg tcacggtgga aggcagttat gaaatcctgc tttcacaacc tgccctttat
     4921 acttccctgg tgaatacagg agatgtggtg cctgtttcat tttctgcttc tgtatgtgat
     4981 tcgcttattc tctaccagaa cggagtacag cttaagaaag tgaccgagac aaccctgagt
     5041 catactgtta ccgccggcgg atcaggaagc tacaaactgc tcgtaaaggc atggcacaac
     5101 aatgtcatga aagctgattc cgcctactat ttcatacgta ctccaactgc ggtggcacct
     5161 gttccggcag ggctgaaacc aggagtgaac atcacgggag acaactcggc ggcattcctt
     5221 ctttatgccc cctacaagag cagcgtcttt gtcattggcg acatcaacaa ctggattcac
     5281 agcagcgagg gtttcatgaa gagaagtcct gacggcaact ggtactggct tgaggtgaca
     5341 ggacttgatc ctgacgagga gtacggattc cagtaccttg ttgatgagac gctccggatt
     5401 cctgatcctt ataccacaaa ggtgcttgac ccatggaatg ataagttcat tgacgaatct
     5461 gtttaccctg gcctcaaacc ctacccggag ggattgacaa atgaacttgt gtcagtcttc
     5521 cggacacgcc ctccggaata cccctggaaa aacaacgact ttacagttcc tgcaagggat
     5581 accctggtaa tttatgagat gctggtgcgt gattttgtgg cgacacacac attcaagact
     5641 gtgcgtgaca cgcttgatta cctgaagcgc ctgggagtta acgccatcga gctgatgcct
     5701 gtgaatgagt tcgagggcaa cagcagctgg ggatacaacc cttcaatgta tttcgcagtg
     5761 gataagtatt acggtacccc tgatgacctg aaagagctga ttgatgaatg tcatggaaga
     5821 ggaatagctg taattatgga cattgtcctc aaccattcat acaattccaa tccgctcgta
     5881 aggctctact ggaacagcac gaacaatcaa cctgcagcga acaatccgtg gtttaatgtc
     5941 acgacacccc attactacag tttcggaggt tccgatttta accatgagag caatgctaca
     6001 aaagcactcg ttgacagtgt atgtcattac tggattgacg agttcaggtt tgacggtttc
     6061 aggttcgact ttaccaaagg gtttaccaat aagaccagta acagcgatta ttcggtatcg
     6121 gcgtatgacc cctcacggat agccattctt aaacggatgg gtgacaggat cagggcttac
     6181 aagcctgatg ccctgctgat actggaacac tttgccgaca acgccgagga aaaggaactg
     6241 gcagcatatg attttttcct ttggggcaat ggaaagtgga gatacgaaga cgcctcaacc
     6301 ggcaactctt cagacatctc tgatgcatcc tggaaacttc tcggttttga caggccgggg
     6361 gtgattgatt acatggagag ccatgaccag gagaggatta tgtacctcaa tcttacccag
     6421 gggaaagctg aaggaggata taatatcagg gatttcaaca ctgccattaa aagggtaaag
     6481 cttacagcca cgttcttcct gacaatacca ggcccgaaga tgctctggca gttccaggaa
     6541 ctggggtatg acgtcatcct cggtgatggc taccagagac ttgaggagaa gcctgtaaga
     6601 tgggattatt atactgttgc cgtcaggaag aatctgttcg acaacttcgc cggtctgata
     6661 gaacttagaa agaagtatcc tgccttctcc tcggacaact tcactctcta ccagagcggg
     6721 atgacgaaga ggctgaacat tgttcattct gacatggatg tggtggttct gggcaacttt
     6781 gactttttcc cccggacgat tgatcctaat ttcacccgca ccggcaattg gtatgagttc
     6841 ttccgcggca cccaggttga ggtgaacgct tctaaccaga atacacccat ctccctcgtg
     6901 cagggagaat accggatcta tacttcaaaa caggttacca ggccgtcgtt cctgcttggt
     6961 atggaggatc cgcttgccga cgacggtgaa ggcctgatgt ttgatgtcta cccgaatccc
     7021 ttccgtgaga gggcagtgat taaattcgcc ggtgacgacc agtatcagcc gcacacggtt
     7081 gaggtgatct ccgccaccgg agctgtggtt cagataatga acacgcccgc gggcatagat
     7141 gatgttatcc tggatggttc tgcacttgac gcgggagtat attacctgaa ggtgacagca
     7201 ggaagggtgt ctgccgtaaa aagggtggta aggtactagg tctgaaggtc tgaaggtctg
     7261 aaggtctgaa ggtcaggaaa attctatcga ccttcagacc ttcagacctt cagaccttca
     7321 gacatcagac ataaaaaacc cggactttgg atccgggttt ttttgtctgg gggaagcctg
     7381 tctgtttact ttaccagtac catatatccc cacggctcca gctgcatctt cctgccctgg
     7441 gcgatctcca cgctgccatc ggtgaaaaga tccctgtagg ttcctgcggt ggctccgtcc
     7501 ttcagcgcca cagtcaccgg cttactgctg atgttgagga tagtcagaac ctcattctca
     7561 ccctttaccc tcttgtatgc gaccacattc ttctggcttg tcttcagcac atcgtatgat
     7621 ccgccttcaa tgccatgacg aagtgccgga tgtgaagtgc gcaggtgaac cagcttcgtg
     7681 tagaagggag taagttcggt ctccttccac tcgatcgggt cgcgcttgaa gaactccagt
     7741 ctcttgtcaa ggtcagcctc ctggccgttg tagagaagag gtacgccggg aacggtaaag
     7801 agatagcagg cgaatgcctt ctgtgcttca cccatcttct cgtcgattgt gccgttccac
     7861 gagttttcgt catggttggt catgaagcgc agcctgtatg actcgggagc ataacgcttc
     7921 agctcatact gaataatgct gtcgagggca gacggtctgc gctggcctcc tgccacggcg
     7981 ttcatcaggt gatgatgcgc ccagccgtag ttcatgtcaa aggcacgctc gagcaggtat
     8041 gaatgctcct catcctctgc tagcatcagc accggcctga ccgagttgag ctcggtagtg
     8101 gccctgaacc agaactcctc aggtaccagt cccgggaagt cacaacggta gccgtcaatg
     8161 ccaacctctt cgacccagta cctcatggca ccaaccatgt aatcccaaag cggctctgca
     8221 gtgtagtcga actggatcac atcggtccag tcaaacggcg agataaattt ccctaaagaa
     8281 tccttttcat accactccgg gaattcggtg gcaagaggat tatcccatgc cgagtgattt
     8341 cctacccagt cgagaatcac gtggaagccc atggcatggg cagtgtcaac aagcgcttta
     8401 aagtcgtcta tagtcccgaa ctcgggattg atccctttat aatcactgat ggaatagtaa
     8461 ctgccgagtt ctcccttcct gtttaattcg ccgatgggat agatgggcat gaaccacaga
     8521 acgttcactc ccagttcctt cagctgagga agtctctcct gcactccggc aaaggtcccc
     8581 tcatcggaga actgccgggt attaatctca tagagaacca tgttacgggt ccagtcggca
     8641 tggacgacag tggtcttcag aggctcaatc accgcggccg gtttcggccg gcaggctacc
     8701 atcagtgttg ctgcaccgat gaacagaatt aaaatcttga aaatgtgctc tttcattgtg
     8761 ataaggttat tttagttcaa taatcattgc tgtctgcggg gctatggtaa acgatttcag
     8821 atcgtcgatc ttccgacctg agatgatatc agttccggag gtgaatcctt ccagtgaccc
     8881 ggcatatttc tcaccgctga tggcacggtt ctccttgctg ttggcgttga ttatcaccat
     8941 tacgctttcg ctgcagtcat atctgaaata gacatagacg ccgtctgaag ggatatagtg
     9001 tttcagtttg ccggtatgta tcacctcctt attcttcctc cagttcagga taagcgttgt
     9061 aaaatcaagt gcttccttct cctgttcagt aaggccggtg cctgtgaata catttctggc
     9121 atcgccgggc catccgcccg ggaagtcatc tctcagccgc ccgtcaccaa ggctcttgtc
     9181 gccttccatg actatctccg tgccgtaata gagctgtggt atgccccttg tggtcatgat
     9241 gaagctcagc cccagcttgt acgcttcgag gcttttgccg gtctgggtgt agaacctcgt
     9301 catgtcatgg ttgtcaagga atatgacgtt cctgagcggt tcggggtaga ggtagtcctg
     9361 cgacagggca ttatagatct tcgagagtcc gtcggtggga ttggtcttgc cttcgaaggc
     9421 cctgtgagtt gcaaagcaga cggggaaatc ggtcaccgat ggcagatttg acctgtaccc
     9481 gtcgctgttt ttcttgttat atgcccagta agagatctga gccgggtcat cataccacac
     9541 ttcgcctaca atgaagaaac cggggaactc atcggtaacc cgctttgccc atcttgacat
     9601 catgtcaggc tgggggtaag ggtaggtatc cattctgatg ccgtcgagat cgctgaaggc
     9661 aatccaccac aggctggtct ggataagata ggtctctaca aggggattgc tctggttgag
     9721 gtcaggcatg gtcacgtcaa accatccttt ttccatcagc atcctgtcgc tctccgcggc
     9781 gtacgggtcc atgtaggtgg aagccctgta attggtgcgg gtgaactcat cgaactgatg
     9841 tatccagtcc tttgacggaa ggtccttcat ccaccagtgg aaagatcctg tatgattgaa
     9901 tatcatatcc attaccactt tcattcctct tttgtgcgct tcggtcacca ggtttttgaa
     9961 ctcgtcattg gatccgtagc gcggatcaga tttgtagaaa tcagttgttg aatagccatg
    10021 gtaggaggag tgctgctggt tattttcaag gaacgggttc agccatattc ctgtaacccc
    10081 cagcatatgg aggtaatcga ggtgatcctc tattcccttc aggtcgcctc cgtgcctgcc
    10141 tcccggcttg ctgcggtcag cctgttccgt catacccgca acgttgtcat tggaggtgtc
    10201 gccgttggcg aaacgatctg gcatcagcag gtagatcacg tctgaggagt tgaatcctct
    10261 tgccggtccg tccggcctgg ccaggagggg atagctgtgt gtaagcttct gcctgccgtg
    10321 ggtgaatgtc agggtcacga ttcccggaac ggcctcatct gagatattca ggttgacaaa
    10381 gaggtagttc gggttttcag tccggaccag agtactgacg tcaactcccg gataatcagt
    10441 tttcacgtcg tatgatccga tctccctgcc gtagaccatc agctgaaggg catcattctt
    10501 catccccgtg aaccatgatg gcgggtcaac gcgttcgata acctgtccct tcgccggcag
    10561 aagtaccgga atcatgaata aaacaagcgc ctgaaatatt cttttcatca tttttcaaat
    10621 ctcctttctt ccccggtttt cagcgtgacc tgctctccgt aaacggcaag aggtatctca
    10681 gcctgtgaga ggttgaccac tatcaccttt gtgccgttga cctcaaccct gaatggattg
    10741 ttccggtatc tgattataaa tgagtatgat tcccacgctg ctggaatgac cggattaaga
    10801 cagacacgct cattgcgtat ccgcatcccg ccgaatccct ccactatggc gagccatgtc
    10861 ccggccatgc tggttatgtg gagtccgtcc ttcacctcac ggttgtagtc atcaaggtcg
    10921 agcctggcgg tgcggaggta cagggtatag gcactctccg tgtcatgcag cctggctgcc
    10981 agcacggagt ggatgcttgc cgagagtgat gattcatgca cgcagcgcgg ctcatagaag
    11041 tcgaaattgc gcctgatggt atcctcgtcg aaatcatcct cgaagaaata gatcccctgg
    11101 agcacatcgg cctgtttgat gaagaccgac cggagaattc ggtcccatga ccagtgctgg
    11161 ttcagaggcc gttcagcggg gtcaaggtct ttgaccgtct gctgcaactt gtccatgaat
    11221 ccgtcctgct gcaaaaagat tccaagatca tggtgcgcag gcaggtacat gttatcaatt
    11281 atctccttcc agtgattcag ttctgcctgt gtatcaagat gtgtgcgggt gacaacggcg
    11341 ttatacttca ccttgctggc tgaccggagc cagtcgagga tgtcagaggt gtatttcagt
    11401 gtccagcgtg ccagcgtgtt tgtataccag ttattgctga cgttgttctc atactcattt
    11461 ggaccggtaa cgccaaggat gacatacctg tttttttcct gtgaccagtt gctcctctgc
    11521 gcccagaagc gcgaaagggc tatcagcacc tcgaggccgt attccgaaag ataatcctgg
    11581 tcgcctgtgt gcctcacata gttgaagata gcataggcga tggcgccgtt gcggtgtatc
    11641 tcctcgaagg ttatctccca ttcattgtgg cactcctcgc cgttcatcgt gaccatcggg
    11701 tacagggctg ccccgcccgt gaatcccagt ttcttcgcgt tctcaatggc tttgcccagg
    11761 tgtttatacc tgtaaagcag caggttgcgt gccacgtgtg tgtctgacgt gctcaggtag
    11821 aagggaaaga cgaaagcctc tgtgtcccag taggtgcttc caccgtactt ttcgccggtg
    11881 aatcccttgg gcccgatgtt cagcctggga tcctcaccgg tatatgtctg gttcagctgg
    11941 aagatgttga accgtattcc ctgctgtgct gctatatcac cacggatgac gatatcacca
    12001 tgttcccact tctgggccca tgcattacgg tgggcctcaa gtaatgcgtc aaagcccttt
    12061 gcggcggcct tgccggccgt agccattgcc ttcgccgcga tcccgtccac gggatggttg
    12121 aaagaggaaa gtaccgcggc atatgtggtc accgtgaagg tgtctccgtc agcacaggcg
    12181 gatgtgaatg cacagtgagc ggttttgtcg gttgtggtaa taaccggttc gccctttacc
    12241 ggcttaccgt tcatggtgaa tatattttcc atcgcagtgg caaccacgaa agctgttttt
    12301 cgcgtctgcg caacaacaac tgagcgtctg ccatcacgtg aagccgattc gttctcccag
    12361 aacttctcgt cgtaattggc atcctcatta tatacatcgg cgttcagcca gggaataagt
    12421 tccaacttac cgccacccct tttgatccgc acgctgtaac tgattgcggc cagttcgggc
    12481 tcatccatcg agaggaaacg cctggcggtc acctctgcct ccctgcccga gggcatcacc
    12541 gctgtgaaca ttcgcgtcag caccccttcc ttcatgtcaa gctccctgcg gaaactcttc
    12601 actttgcact ttacaaggtc aagctcctca tcatcaatgc gtactattat gtttgtccat
    12661 gaaggcgcgt tgggaacctt ggcaaagtat tccgggtaac cgactttcca ccatccgaca
    12721 acggtcttgt caggataata aacaccggcc acgtagttgc cccggagtga gtcgccggta
    12781 tatgtttctt caaagtttgc cctctgtccc atcattccgt tacccagggc aaatatgctc
    12841 tccgaatcgc ggcctctcac gggatcaaac cgtgtctcga tgattttcca ctcatcgggc
    12901 tgaaaacgtg aatcagtcat tttgaattat aagtttctca attgtttcaa actaatttta
    12961 ttaagaccgg gaatgacgag atcagccctg ccaagcagct cggggctgcc tactcccaca
    13021 cacttcattc cgccggcgat tgccgcctct atacctgcct gcgcatcctc gaaaacaatg
    13081 cacttcgagg gcgacagctt catttcctgt gcgcccctga ggaatacctc ggggtcaggc
    13141 ttggccttcg atatcttgtt tccgtcaatg atcacatcaa acatatcacg aaggttaatg
    13201 ccgtcaagga ttgttccggc gtttttgctg gccgagccga tggctgtcag gatgccctct
    13261 ttcctgagcg ttttgaggag cttcacgctg cccgggagga tctcatccgg tttcatgccg
    13321 gagatgaact caacatacca gccgttcttc cttgccgcca gctcctcctt ttcctgtatg
    13381 gtggcatgga tgtttccctt gctgagaagg atctccagtg aagccatccg gctgactccc
    13441 ttgagagcct cattatcttc gagggtgaat ataaaaccga gctcctttgc caggcgtctc
    13501 caggccatga agtgatattt ggcagtgtcg acgataacgc cgtcgagatc aaaaatgcac
    13561 cctttcagat ccatcatcac ctcctgtcgt caacgtcttt gacaaaatta accgcaagag
    13621 ccgcaattat cattgagatc cctccggtca ccagggccag catcgtgttg cctgagaaaa
    13681 ggtgcttgac catgcttccg aggatgctgg ctgcaagtat ctgcgggata acgatgaaga
    13741 agttgaagat gcccatatag atgcccatct tgttgtgagg cagtgatccc gtcagtatgg
    13801 cgtaaggcat tgacaggata cttgcccatg ccagtccgat gccaagcatc gggaggatca
    13861 gcatgccggg tgaattgatc aggctgattg acatcaggct caccccgccc accaggaggc
    13921 agatggcatg agtgaccctg cgtgatgtgt attttgccag gaccgggagc aggaaggcaa
    13981 ccagtgctgc aaatccgttg taaaccccga agagcactcc cacccagttg gcgccttcgt
    14041 tataggcaag ggaggcggtg tcactcgtgc cgtatatttt tgaggtgaca gccgatgttg
    14101 tgtaaatcca cagggagaag agggcaaacc atgtaaagaa ctgcaccacg gcaagctgcc
    14161 ccatggtggc gggcatatgc ttgaggtcgg tgaggatgct gttcagcccg ttgctgccct
    14221 tgccgccggc cttcaggaat cctgcaatta tctggagcaa tccgaagagg gagatacctc
    14281 cggtaacaac gtaaagttcc ttctcgaggt taacctgcga gaggatgatg gtaagcacca
    14341 ggcctacgga gaaccatatg atgccgggac ggtaaaaatt gatgtccctg actgtttctg
    14401 tctgccctga cacggttttc tctgcgagag gctctcccct ggctgtcttt tcgccttttt
    14461 caaactcctc gagttcggca ggtgagtatt cttttgtacg gattatggtc cagagtactg
    14521 caaggagcaa cactgcagaa ccgatgtaga atgaaagttt caggttgaat ggtatcagct
    14581 tgccctcggc gggaacctca ggtacattga accagttggc cagtatccag ggcaggaagc
    14641 tgcctattac ggcgccaacg ccgatgaaga agctctgcat tgcgaaccct gatgttcgct
    14701 gttccgatga gagcatgtca cccacgaatg cccggaacgg ttccatggaa atatttatag
    14761 aggcgtccat gatccacagc atcacagtgg caaaaatcca ggtgggtgag ttggtgatga
    14821 tggccagtgc gagagaggca agaatggcac cggtaaggaa ataaggacga cggcgcccga
    14881 gacggttcca ggtacggtca ctcatgtgac ccacaattgg ctgtaccagc agtcccgtta
    14941 cgggagccgc tatccagagt attggtatat tgtcaatctc cgcgcccaga gtctgaaaaa
    15001 tacgactgac gttgccgttc tgaagggcga aaccaaactg gatacccagg aatccgaagc
    15061 tcatgttcca aatctgccag cctgacaacc gtggcttgcg tctgtagatc acattcataa
    15121 tagttttgct gttagtttat taccctgttc cgataaccta ttcaaaatta gtcgaaaatc
    15181 cggttcggca atagcttcat cgtgcttcta aacgctgatt tttcgggcat ttgcgttggt
    15241 tcagttattt caaacgtttt cggcctgagg cataattttt acagagtctg cttatttaga
    15301 cgcgttataa ataatatatt tcactgttgc cttcccgaac cgccagttgg cggatacggg
    15361 attgatccgc ctcctgtgtc agcgtctcaa cttgctcttg ctatttgcct cctgcgttcc
    15421 tatctttgat agagtaaagc ccttaaaaac aaaacagcca tgggagattt caactatgaa
    15481 aagccattcc agcacagcag ggatgtgaca aagtatcgcc gcatatctgt tgatcatgtc
    15541 aggatgaccg aatgctgcgg caggaagata ctgcaggttc agtccgaagc tctcaggaca
    15601 ctggcacggc aggcatttgt cgatgtaaat ttttacctcc gtacgatgca ccttgaaaag
    15661 ctcgcgatga ttcttcgtga tccggaggcc acggacaatg atcgctttgt tgcgtgggtg
    15721 atgcttcgga actcggtcat ctcgtcggaa ggtaagcttc cgtggtgcca ggacacaggt
    15781 acagccattg tgatggcctg gaagggagag gatgtgtata cgggaataga tgatgcactc
    15841 catctcagcc gtggcatatg ggagacctac aatgaaaaga atctgcgcta ttcacagata
    15901 gttccctctt cgatgttcgc cgagaagaat accggaagca atctcccggc acagattgac
    15961 atcgcctcgt gtccgggaga tgagtatcgt ttcctgttca tcgccaaagg cggtggttct
    16021 gccaacaaga cctttttata ccagcagtcg aagtcgctgc tgaacgaagc ttcgctcagc
    16081 aggttcatcc gggagaagat tgaggatctg ggcaccgcgg cctgtccgcc ttatcatctt
    16141 gccattgtca tcggcggcac ctcggccgag atgactctca agaccgtgaa gctggcctct
    16201 gccggttacc ttgatgacct gccaacctcg ggcaattctg ccggccgcgc attccgtgat
    16261 ttggaatggg aggagagggt tgagaagata tgtcaggagt atggtgtggg tgcacagttt
    16321 ggcggaaagt attttgtgca tgatgtcagg gtaatccgtc tgccgcgtca tgccgcatca
    16381 tgcccggttg gcataggagt cagttgcagc gccgatcgca atatcaaggg gaaaatcact
    16441 gccgaaggca tcttcattga gcagctcgag acagaacccg ggaaattcct tccggagaag
    16501 gctccggagc ttgataaacc ggtggtgatt aaccttgagc gcccaatgaa ggagatactt
    16561 gccgagctga gcaaataccc ggtaaagacg cgccttagtc tcaccggtac cctgattgtt
    16621 gcacgcgaca tggctcatgc gcgtatcaaa cagatgatcg atgagggcaa gccgatgccc
    16681 gactacttcc aaaaccaccc ggtatactat gccgggccgg caaagacgcc ggaggggatg
    16741 gcctcaggct cattcggacc cacgacggcc ggccggatgg atccgtatgt tgaactcttc
    16801 cagagtctcg gcggatcaat gatcatgctt gccaagggga accggtcgaa acaggtgacc
    16861 gatgcttgca aaaagtacgg cggcttttac cttggttctg tcggaggtcc ggccgcaatt
    16921 ctggctgctg aaaacatagt atctgttgag gtggttgact tcgaggagct tggcatggag
    16981 gctgtccgga agatagtagt caaaaatatg cctgctttca tcctgacgga tgataagggg
    17041 aatgattttt attcgggaat aaaacaatga ggaattgcga tttgcgattt gcgatttgcg
    17101 atttgggatt tggggttgcg aattgcgagt tgggaattgc gacctgcgat attgatctca
    17161 acgtttcccg gataagtata taaataatac aaaaaagcgc aaacaggtta aaaaacatta
    17221 ctaaaacgcc ccgcaaacaa caaaaaacaa ttacatttgc tccctttaac tcatatttca
    17281 ttaaattatg tgtggtgttg ttggtgtttt tgacctgaaa atcagggcgg gggagcttcg
    17341 gcagcaggtt ctggggatgt ccggtaaact gaggcatcgc ggcccggact ggtcaggtat
    17401 ctattgtgga gaaaaggcaa taattgccca tgagcgactt tcaatagttg atgtgcagtc
    17461 gggccgccag cccctgtaca gccggaaccg caagcttata ctcggagtaa acggtgaaat
    17521 ctataatcac cgggagatcc gggagcgata cagggacagt tatgatttta caacgaagtc
    17581 agactgcgag gtgattctcg cactctacgc tgacaggggc tgtgatttcc tggatgaact
    17641 taacggaatc ttcgcatttg tgctatatga cgaggagaat gactgttacc tggcagcccg
    17701 tgaccatatt ggcatcatcc cgttctatat gggatgggac atgtacggca acctgtattt
    17761 cgcttctgag ttaaaggcgc tggagggcgt ctgcaagaag atagagactt tcccaccggg
    17821 ccattacctg tacagcaggg atggcgtcat gaaaaaatgg tataatcgtg actggacctc
    17881 ctatgataaa gtggaagaga acagcactga catcgtcgcc cttcgcgagg cgcttgaaaa
    17941 agctgttcac cgccagctga tgtcggatgt accgtatggt gtgctgctgt cgggcgggct
    18001 tgacagttca gttatatccg ctatcgcgaa gaagtatgcc gcccggcgca ttgaaacaga
    18061 tgaccaggct gatgcatggt ggccgcaatt acactccttc gccatcggtc tggagggatc
    18121 gccggacctg gcagcggcac gaaaagtggc tgaccatatc ggtacggttc accatgagat
    18181 acatataacg gtccaggagg gcctggatgc ggtccgcgac gtcatatatt accttgagac
    18241 atatgacgta acaaccgtca gggcctcaac tcccatgtac ctcatggcca gggtgataaa
    18301 atccatggga gtaaagatgg ttctctccgg ggaaggggcc gatgaggtct tcggcgggta
    18361 cctttatttt caccgggcgc cgggcccgcg cgagttccat gaggagacag tgcgcaagct
    18421 ctccaggctc catctctatg actgccttcg tgcaaacaag tcgcttgctg cctggggagt
    18481 ggaggggaga gtgccattcc tcgacaagga gtttcttgac gtggctatga ggcttaatcc
    18541 ggccgacaag atggcaggta acggcaggat ggaaaaatgg attctgagaa aggcttttga
    18601 ggattacctg ccggatgata ttgtgtggcg tcagaaggag cagttctctg acggggtagg
    18661 gtacaactgg attgataccc tgaaacagct gacctccgaa aaggtcaccg atgagatgat
    18721 gtcaacagcc cgttaccggt tccccctgaa cacgccaatg accagggagg agtatttcta
    18781 ccggtcgatt ttcactgagc atttcccgtc tgaaacggct gccctgacag tgccatctgt
    18841 gccctcggtg gcatgcagca cggcagaggc gctggcatgg gacgaggcat tccgcaacca
    18901 gaacgaaccc tcgggccgtg cggtgcgcaa cgtgcatatc aaatcgtggt aaccatctcc
    18961 cgtacagaaa tccctgtaaa ccagaacgta acggcaggtt ttagacctgc cgccacgtgg
    19021 tcaaaacata attatttctt caatatggtg tccaggatct tttgtttgtc aagtttcttg
    19081 gtgcagaaga accagtcgta gcacttcatc tcctccttgc tctccatgcg ctctttggca
    19141 acgccgcatg aaccggctgt gggaacgatg acatcatcac ccggctgcca gtcggcgggc
    19201 agagccactg agaacgcgtc tgcagtctgc aggcccagga ccaccctgta tatctcctcg
    19261 aaattgcggc cgaggctcag gggatagtaa agaatggtgc ggataacacc cttgggatca
    19321 atgaagaaaa cggcgcggac agccttggtg ctgctctcgc ccggcatcat catcccgtat
    19381 ttctgggcaa ctgacattgt aatatcctcg ataagcggga atttcacctc cacatccttc
    19441 atacccttgt actcaatctt ctccttgatt gtgcggagcc atgcaatatg gctgtaaaga
    19501 ccgtcaacag acagacccac cagcttcgtg tttgcctttt cgaactttgc tgccatggag
    19561 gcaaaggtca tgaactccga ggtacacacc ggtgtaaagt cagccggatg gctgaagagg
    19621 atcacccagc tgccggaata gtcagaaggg aaattgatct caccctgtgt ggttactgcc
    19681 ttgaattcag gagccttgtc gccaatacgc ggcattgaat aaattttttc ttccataata
    19741 aattgatgtt tagttaatta attgttattg ataattttta ttttcttctc tcaatcatca
    19801 gatcaaagtc ctccttcagt gcaacgaggt cctcttcatg ttcaacctca tcggtgagga
    19861 tctgaaggat gatgttgtag gttatcgggt ctgcctccct ggtaagagca aggagcttgt
    19921 tataaaccct gatagcgcac tgttctccct tgatgttctg gtcgagtacc actgatacga
    19981 aggggtcttc cggggcgtca tagccgcaat ttgtgatttt agtccactcg ttgggtgaaa
    20041 gcaccggtgt gccgccaagc tgaatgatcc tgccaactag cagctgtgcg tgtgtaagct
    20101 cctccgttgc gtgaagctca agctcagcta ttaccgcatc tttcattggt cccttgacaa
    20161 cctttgcgcc gatccagtac tggtagtagg ccagccactc gtcagccagg gctttgttga
    20221 gtagagtaag aagctcctca acgtccatcc ttacgattga tcttccgatt tctgccataa
    20281 ttctttgaaa tttaaacgtt taaaagttaa tgtattttca ttgtgtcatt tagcccgggg
    20341 aaacttgatg ccgagacctt tggccaccag ctgtgccagt tccttgtcgg ccttatgaaa
    20401 gtgctcaagc tgccgcatta gtatttcctt cttcttcgga ccggaaaccc ccttgagggc
    20461 gcagaccagg ttcccaacgg tgttggctct ctcctggtcg gtcatcacct tcctgaagag
    20521 tgcgcctgcc tgagagtagt ggtcgttgtc atttttattg cggtcgaagt agtcggccac
    20581 atcagaatca agagccatgg cagcttccct gtaattcttg tcagcctcga tgttgtcaaa
    20641 gctgttgggg aagtagttgg ggccggatcc ctggttgccg tcaaccgtca tgtgcccgtc
    20701 gcgctgatga tggcttacgg gcgctatcgg tctgttgacc gggatctgct ggtagttggc
    20761 tccgagcctg tagcggtggg catcggggta tgcgaagata cgtccctgga gcatcttgtc
    20821 aggcgagagg cctattccgt cgactgtatg cgcaggggcg aaggctgcct gttccacatc
    20881 ggcgaagtag ttttcaggta tctcattcag ttccatgacg cctgcctcga tcagcggaaa
    20941 ctccttgtgc ggccatacct tggtcacgtc aaacgggttc cacttgaact tcttcgcctg
    21001 tgcgtcagtc atcacctgga tcttcagagt ccatttcgga tagtctttgc cggcgattgc
    21061 atttacaagg tccctctgcg agtagtcggg atctttgccc ttaatctcct ctgcctgctg
    21121 gcgggtgagg gttttgttgc cctgctgtgt cttgaagtgg aatttgaccc agaccctttc
    21181 attcttttta ttatacattg agaatgtatg gctgccatag ccgttcatga acctgtagcc
    21241 gtcaggagtg cccctgtcac tgaagagggt cattaactgg tgcagacttt cggggttgag
    21301 gctccagaag tcccactgca ttgtgtcgct cttcaggttg gttttcgggt cacgtttctg
    21361 ggtgtgaatg aagtcgggga acttctttgc atccttgatg aagaagaccg gtgtgttgtt
    21421 gcccacgagg tcccagttgc cctcttcggt gtagaacttg acggcgaaac ccctggggtc
    21481 gcgttcggtg tcggcactgc ccttctcacc gcctactgtc gagaagcgga cgaagacgcg
    21541 gcatttattg cccaccttgc tgaacagtct ggcgcgggtg tatttcgtta tgtcacccgt
    21601 tacggtgaaa gtgccgaaag cacctgtacc cttggcatgc acaaccctct cgggtatgcg
    21661 ctcacgattg aagtgagcca gtttctcgtg gagataataa tcctggagaa ggaccggtcc
    21721 cctcggacct gcggtcatgc tgtcttcgtt ctcgaagtac ggcctgccgg ctgctgttgt
    21781 aagcttcttc tttttcatat ttgcggaggt ttttaatgat tgctgtctgc cttattattt
    21841 ctctggctga aaaaatacct tatcttgaag attgttcctg ttactccgac cgcaatcagc
    21901 gctgttttca gccatatgtt gcttacccag aggaagaagg taagaaggat cattgtccac
    21961 atgatggcca tcgcccatat gccggccttc ttctgtgccc gtggagtaac gtaggccgat
    22021 gtgatcctgc cgttgacaac cctgcggtag agttcagggg agctcttgac gtagaggcct
    22081 gccgagagga gcacgaaagg ggttgtgggc agcacgggga tgaaaatccc gatgataccc
    22141 agtgccagtg atactgtccc ggctgatatg agcaggagcc ttatcatctc ttcctgatcg
    22201 tctccctgtt atcagtgtat tttccgacga tctgcagctt gatatcctca atcctgaaac
    22261 cgggaatacg atggatttca aaataatcat agagcatttt cgtcagcccg gcgtcatagt
    22321 aatcctctat gcggtcacag tgggtgcagt aaatatggtg atgcctgtcg tcaacagcat
    22381 catacctgag aaggccgctg tcagtcttga ccctcctgag gatggctttt tcagccagtg
    22441 tctcaagagt tttatagatg gtacccagtg ctatttcagg gtgttttttc ctgatatact
    22501 gactgacctc ctcggcggtg ggatggtcat gaagcacatc tactgcctca taaacagcga
    22561 gtctctgggg tgttaccctc agcccgctct ccctgagcct ggttccgtag tcttcaattt
    22621 tcattaagta agaataattc ttaacaaaga acaaacaaat ataagccgga aaagttacaa
    22681 aacaaaatat ttcttgcccc gttctgttta gaatgtcact gcagggcagc aattcatccg
    22741 ttaagctaaa cagaatataa tttatcgagg acgtccagaa taatctcgtc aacattttcc
    22801 cggttgtcaa ttgccacggt ccgggaaaca gacagccctc ctgttcatgc tttaacattt
    22861 ttgaaaattg gttgttatga tcaaagttag gctatcataa aggcgatgtc aagtatctga
    22921 tttatatatt tatcgttatt tttgtggcct gaaaaaacga gagatggcaa acagaaaatc
    22981 actggcactt gcagcgttgc tgctgataca cacggcgttc gtcaatgcac aggtccttga
    23041 accggtggcg tggagcttca aaagcgagaa gaccgcggaa aacaattatg atattgtcat
    23101 gacagccacc atcgataata catggcatct ttacgccatg gacctccccg agggagggcc
    23161 ggtcgccacc agttttacct ttgaaacgcc cgtgggctat accctggaag gcaaacctgt
    23221 agctgtcaat cagcccgaag tcaagtttga caacagtttc gggatggata tagggatgca
    23281 cagcggcaca gttgagttca gacagaagat aaccgtcagt aagctgccgg taactgtcag
    23341 cggtttcgtt acattcatgt catgcgatga cacacagtgc ctccccccgc gcgatgtcga
    23401 gttctcggtc aatatagggg gtgaggttgc agcatccgcc gcttcagccg aacaggcagc
    23461 aggcaccacc gtaccggctg acaaaccccg caagggtttc ctgaagttct tccttctttc
    23521 gatgctggcc ggcttcgccg ggatactcac cccgtgcgta ttcccgatga tacccatgac
    23581 agtggcattc ttctcacagg gaaccggatc aaagggttcc gctatcggga aggcgctcat
    23641 attcggactc tccataatac ttctttactc gtctctgggc ataatagtat cactcaccag
    23701 cgccggcgcc gggtttgcaa atactctcag cacacactgg atccccaatc ttcttttctt
    23761 tacactcttc ctggtatttg ccgcttcgtt ctttggcgca ttcgagattg ttcttccctc
    23821 aaaatgggtc agcacatctg actccaaagt ggacagggga ggcgcactgg ccgccttctt
    23881 cctcggactg acaacagtgc tggtatcatt ctcatgcacc ggaccgatcg ttggcgcact
    23941 gctcgttgag gcagcatctg gtgatgtcct ccgtcccacg atagggatgt tcggattcgg
    24001 cctcgccttt gcattgcctt tcaccctttt tgccctgttc ccctcatgga tgcagaagat
    24061 gccaaagtct ggcggatggc tcaactcggt taaggtggtg cttggattca tcatgctggc
    24121 cttcagcctc aagttcgcat caacggtcga tacagtctat aaccttggca tcctctcaag
    24181 ggatatctac ctggcaatct ggatcgtgct gttcgtgctg cttggcatgt acctgatggg
    24241 taaaatcaaa ttttcgcatg acagtgacgt gccacatatc ggtatcttca ggctggtgct
    24301 tatcatcgcc tcattcacct tcgccctcta ccttgtaccc ggtcttttcg gtgcaccgct
    24361 gacgcgaatc tctgctcttc ttccccccag ggagacatca ggcttcaacc tgctcaaggc
    24421 catcggcgaa aacaggggga cagccgcacc gggagccctt gtaccgggaa ccctggcgcc
    24481 ggggactctt gcaacagccg gagcagccct ctgcgaggaa ccaaaatacg gcgatttcct
    24541 tcatttcgaa tacggcctgc agggctattt tgacctggaa cagggtctcg catgcgcgaa
    24601 gaaactgggc aagccggtgc tgatcgattt caaggggcat gcctgcgcca actgcaagga
    24661 gatggatgca cgggtatggt ctgatcccgg agtgcagcag agactgcgcg acaactttgt
    24721 tctggtggcg ctgtatgtcg atgaccgcac taaattgcct gagagcgagg tttatgtttc
    24781 aaaggttgac ggcaagcaaa aaaagacaat gggcaagaag aacgaggata ttgaaatctc
    24841 aatgttcaat accaacaccc ttccgctcta tgccatcgtt gacccggacg gtaagccgct
    24901 cacagagacc cgcggaacca atttcgacat ccaggcttat atcgactggc tcgacagggg
    24961 ggtgaaggca ttcaggaatc aataacctta ttttgacatg tagaagacca ggctgccgcc
    25021 gttcattatc tcgtcatggg ttatatagtg gcggtcaagc ttcgtgccgt taagttcagc
    25081 ttttttaacg tatatgttct tcgacccctg gttcacagcc gtcaccgtga atttctttcc
    25141 gttctcaagt gctatggtgg cttcctttac gagcgggctg ccgaggtcat accggtctga
    25201 gcccggagcc accgggtaga aacccagtgc gctgaagatg taccacgcgc tcatctgccc
    25261 gcagtcgtca ttgccgctca gaccgtccgg tgccggcagg tacttggtgt ccatgatctg
    25321 tctcacccgg tactgggtct ttcgaggtgc gccggcgtga ttgtacagat agggtacatg
    25381 gtgcgagggc tcatttccgt gaacatagtt gcctattatt ccctcgcggg tgatatcctc
    25441 ggtttccgcg aagaaactgt caggcaggta catggtgaag agtgaatcga gatgttccgt
    25501 gaatcttttc ttcccgccgt ggaggcggat cagctctgcc gggtcatggg gcacatagag
    25561 gctgtagttc catgcatttc cttcgatgaa tccctgaccg tgcgttgaca gcacatcaaa
    25621 gtccttcctg aagctgccat ctctcttcct ggggcgcatg aatcctgttg catgatccca
    25681 tacgttcatg taattctgcg acctctccgc gaactcccgc ctgaggtctt cgctgccggc
    25741 tatctgtgcg atgcaccagt cgtcataggc gtactccagg gtgacggaga ccgatgatcc
    25801 ggttcgttcg tcaggtacgt atcccagttt catatagtcg tcgaggccgt cataccagct
    25861 gttgcgggca gtggtgacgc aggcagcggc ggcccttgca tgatcaaagc ctccgatctt
    25921 cttcacgatg gcatcagcca gcaccggcac cgagtgatat ccgatcatgc accagttctc
    25981 attggcatga tgtgaccaga cgggaagcat gtgatggacg ctctgatcat aatgtgccag
    26041 catcgaattg atcatgtcag ccgtgcgttc ctgctggatg atggtgaaga gcggatgcag
    26101 cgccctgtag gtatcccaga gtgagaatgt cgtatagttg gtgaatccct ccgcacgatg
    26161 gatgttctgg tcgagtcccc tgtagctacc gtcaatatca gtatagacgg tcggagagag
    26221 gaaagtatgg taaagtgagg tgtagaagct tgtcatctgc ccttcatccg ccatttctgc
    26281 cttcactttt ccaagctctt ttctccatga tgcttcggcg ccaatcctgg cagcgtcaaa
    26341 gtcagcccgt ggcgcctcgg caatcatgtt ggccattgcc ccggctgagc tcaccggcga
    26401 gatggcgacc ctgacagtga tctgctcacc gggttctgca tcgaaatcaa aatgccccct
    26461 gatgttgcgt cctgctgctt cgggaaaatt ggtccgctgg tcaaagcggc gccagaatcc
    26521 cctgtacacc ctggcaggag agtcatcgcg gaagccgtat gatgtgaaag gtttggagaa
    26581 agcaattgcg aaatggagcg acctggtgcg ggcccagccg ctggtctgcc tgtagccggt
    26641 cacgagggtg tcattctcca cccgtatgaa tgtccagacg tttttgcccg cgtcattgta
    26701 gatgccgtgg accatgtcaa ggatgaggtg tgcatcacct cccctgttaa aggtgtaacg
    26761 gtggacccct gtgcgggtgg ttgccgtcag ctcggcaagt atgtcatggt cttccagaag
    26821 aactgaatag tagccgggtg aggccttttc attgctgtgg gaaaatagtg agcggtagcc
    26881 cttttcgggc gcatctgccg tgccgggatt cagctgcagc ggccctgtgg tgggcatcag
    26941 cagtatatca ccgaggtcag aatggcctgt accgctgaaa tgggtgtgtg agaatccgac
    27001 gattgttttg tcagcatact ggtaaccagc gcaatacctg taaacatccc tgttgtaact
    27061 gccgttaacc tcgtagggaa gtgtgtcagt atcgggtgaa agctgcacca tcccgaacgg
    27121 tactgtggcg ccggggtagg tatggcccat cttatcggtg ccgataaagg gattgacata
    27181 tttcaggatg tctgcatcct gtccctggct tatgacggtt gccatgctga tgatcaatgt
    27241 ggtgaggagt ctgcctttca tatctggtct gattgcagcc gtaaaggtaa aaatattggc
    27301 tgcaattctc aggcatgaag gcaggggagg cgggagtctt tacctctgtt cctggtattt
    27361 caggacctcc tcgttgtatt tcctgttttc gaggatgcgc acatatctga gtccgtgttt
    27421 gttgccataa tctgaataag ccttttgagc ccatgatgtt gcagcatcca ggtctccgtt
    27481 tatctcactg ataatagcca tgttataatg tgcccttccg gcgatcttca tcttcgggtt
    27541 tgtggtctcc acctgccaca actcggctgc ttcgtcccat ttgcccagtt gtgcacgccg
    27601 ttttgcgatc ttgaagctgc cagtgccctt gacgaaatag tcacgtgtca cccgcagacg
    27661 gtatgggatc aggcggatgg catagatatg accggctttg cggctcacct ccttcactgc
    27721 ttccttgcgg ttgaccatgc cggcaacggc tgccaccgga ttgatcccct ttcctgcaaa
    27781 cacgattgat tctcctacaa tgaattcatc gagtattgcg cggtctgagg gagaatagat
    27841 tctccagccg gtttttacaa gggtttccat ggaggcgatg tggttcagcg ccggtatcga
    27901 gccgagcgga gtcttgattt ttgtcggctc ggtggaatag ttgactcttg tgtcggtatc
    27961 gtacatttcg agggcgaaga gtgcctgtgt tccgttctcg ttacagatca tctcaacctg
    28021 ttcccaggtc agcgggaccg gcagtccgcc caggctgctg gtcctgaact gcagtccgtt
    28081 gagaagctta acctcgttga agcggtcatt tgcagcaagt tcctccgtga cgcctttgat
    28141 ggcctcccgg gtgccgatgg agtcaaggtc agttccttcg agtgacagca gtttgtcgag
    28201 gttgtcgagt gatttggtct ggtcggttgg tgttgagcgg tcaatgatcc ctacggtttt
    28261 tatctccggt gcgatggtta ccggggccgg ctgggtcacg ttaaggaaga gctcctgggt
    28321 ggcgcatgag gccatagccg cagcaagcag tggcaggata aggtacttct tcatgacgga
    28381 cagtgtttat ttatccaaag gtatacaaca tggaacaata acaaatattg cttatccggt
    28441 gttcgcaaca gttccaaatc cggcagccgt gaggctgccg gggtgggagt ggcctgcccc
    28501 gtctgcaata ctttatgtcc tatagcccaa atcgcaaatc gcaaatcgca aatccacaat
    28561 gctttatggc ctacagccat ctaatcaaca gccaactatt tactccaggc ttcccactgc
    28621 cttctccacc tcatggagta tctcgcatga tttaacgagc tccttcatgg tggtgtggtc
    28681 agcatataga ggacggtcaa tatcgaggaa atcgacgtgt ttgcggatca catccttggc
    28741 tttctgcacc ccggttccga agctgtactc gctctttctg aagtccagtg cctgtgctgc
    28801 agccatatac tctatgccca ggatgccgta tgcattgtca aggatctggt tgttcttaat
    28861 ggccgtgttc attcccatgg agacaaaatc ttcctggtca gcggcagccg ggattgattg
    28921 aattgatgcg ggtgctgaga gtatcctttg ttccacaatc atcatgtcag cagtgtactg
    28981 gctgagcatc agtcccgaga acatacctgc gccttttgta aggaatgccg gcagtccgac
    29041 gctgagggcg ggattgttaa gcctgttcat tcttctctct gacattacgc tcaccatggt
    29101 gacggaggca cctgccatat ccatcggtac ggcaacaggt gatccctgga agttggcacc
    29161 ggagagctgc aggtttttat cagggaagaa tatcgggttg tcaccaacgc cattgagttc
    29221 aatctcaacc tggctgcgtg catatcccag catatcatgt gcagcgccga taacctgcgg
    29281 ggttgatctc attgagtagg catcctgtac ctttgacttg acacgtcctt ccttcaggtc
    29341 gccgcccgaa acgcatttat tgattgcatt ggcgctgcgg atggctcctt tgaaaccgcg
    29401 tgcctcatga agaagcggag catatggttt catattggcc ttgagagcct cgagtgacat
    29461 ggcagcggct atctcagcct gtttcagcca gttattggca tcaaccagcc agatggcgct
    29521 catggcggtg agcatattcg agccgttgat tgctgccagt ccgtcgcgtg ccagcagtcc
    29581 gggtactttg atacctgccc ttttcatggc ttcggcgccg tcgagaagtt ctcccttgta
    29641 ataggctttg ccttcgccca tgaggaggag tgctatctgg gacattggag ccaggtcgcc
    29701 gcaggcgcca acagatcctt tctggcatgc atacggtgtg acacccttgt taagcatctc
    29761 cacgtaagtc tcagtgatct caagacggca gcccgagttg ccgtgggcgt gcacgtttat
    29821 acgaccgagc attgcaccgc ggacaacctc aatcggagcc gggtcaccaa tgccggcagc
    29881 atggttatat acgagatatt tctggaagtc cttgatctgg tcatcattga gaactatctc
    29941 ggaaaactcg cctatgccgg tgttaacccc gtacatgatc tcatgtgcca cgatcttttt
    30001 ctcaagcatg gcgcggcatt cgagaatctt cttgcgggca tcgggatgaa gttccacctt
    30061 ttgtccgtgg cgggcaacat tgacaacgtc attgacggtg agccctgaac ctttaatgat
    30121 taatgtatcc atagatataa gatttttatt tttcagaatt gatgattaat ggcttgttta
    30181 gaaaaagcaa agaggagcgg cgtcaccgtg gtgtgtcgct ttgcgggagg cattcaatac
    30241 aacccggagc ggttgatttt gatcttgtat tccagtaccc ggctgcgtgt catgatgcag
    30301 atttacagca ccaaatatat gaaaagaaat gaatttgcca tgtgacgtaa gttacaggtg
    30361 ttttttttct ctcatatatg tcacaattct acattccgcc ttgatttttt tcctattttt
    30421 taggttttaa ttctctgacc tatgaaaagc agatttacct caactctgct gctggtaatt
    30481 gttcctgcag caatactcct tacactggcc gcctttaaca taatcagatc gctaccgcag
    30541 gaagatctga agggaaagta ccagtacaag gatttcgagc cggctctcaa gtgccgctca
    30601 tgtcacccgg gcatctatga gcaatggtct caggcgatga tgtcacaggc ttacacccat
    30661 cactgggatg aaattgaata ttttgaactt gcagttcccc atgcagaagt taatcctgcc
    30721 ctgaaggatc cggttgacgg ctgcaatggg tgccatacac cgatggcata tatggtcggc
    30781 aagtttcctc ctccgcgccc gggggaaaag agcatggcca atgagtcagt ctcgtgcgag
    30841 gtctgccatc ttacgcagag ggcacaggtt gatccgccct tcaatttcag ctactttatt
    30901 gaacccgggc ggacaaaata cagcggtcgg gagcctgaaa tccagtcgcc tgcacacaag
    30961 attgttcaga atgatttctt ccggacaacc gagttttgcg gcaactgtca caacgagaag
    31021 aatccttacg gcatctgggt gaaaagcacg cagctggagt ggaaggaggg accctatgcc
    31081 aaagagggtg tacggtgtca ggactgccat atgccaaagg gtccgtatat cctggcactg
    31141 atggggaagg agcgcgatga tgcccgcctc cacctgttcc acggagctca tgaccccggc
    31201 aaggtaagag gaaccattga gctacggatt gagcctgacg tacgtctcgc cgagcccggc
    31261 gaaaacatag tctttaccgt tgcgctgttc aaccagaaga cgggacacaa gtttcccacc
    31321 ggatcggtgg aagaccgcat agcctggctc gatgtcgagg ctactgatgc taagggtaaa
    31381 acctatcacc ttaacgttga taaaaaagga tttgaaggcg aggagtatac tatctctggt
    31441 gattaccttg cctaccagga catggccata ccgctgaagc ttactgattt caggggagtg
    31501 cagcgcgacg gcataccata cgggaaccgg atattccgta tgccctattt tgatgagcag
    31561 ggaaatatga caatcatgca atggaacacc ttctcccagg gagtggatta ccgtatcggt
    31621 ccaagggaga ccaaggtgga gaaattcaca ttcaaaatac cctatgaggc tgcacccgga
    31681 gagatgaagg taagagctgt gcttaactac cagctccttg tgaagccggt ggctgactac
    31741 ctgaaagtgc cgtctgatga gtcagagatc ataatggtca atgagcattc aaccagagtc
    31801 gagattcttc cctgataatc aaaccttaaa gaaatttata tgaaaacttc tttctatgcc
    31861 agggctgcca tattgaccgc aacgatcatt atgctgcttc catatgaaca ggtatcggcc
    31921 ataccggcct ttgcccgcaa gtaccagatc agctgccagg tgtgtcattc tccggctgcc
    31981 cctaccctga agccattcgg cgatgagttc gcgggagcag gcttcagaat gactgattat
    32041 gaatcgccac gctatttcat acaaaccgga gacgacaggc tttctttgtt cagggagctt
    32101 ccgcttgcca taagaattga cggatttgct acattgaact tcaatgatgc cggaacagtt
    32161 gatttcgggt cgccgtttgt ggtgaagatc ctttccggcg gtgagctttc cgacaagttg
    32221 tcgtactact tctatttcct gatgaacgag cgtggtcaca tagccggtct cgaggatgca
    32281 tttcttatgt attccgatgt gctcaattca ggcataaacc tgtttatagg ccagtttcag
    32341 acttcagacc cgctgttcaa gggcgagtta cgccttacac tcgaaccgta caagatttac
    32401 ggcgtcaaac catcagaatc ttcggttgac ctgaagtatg accggggaat aatgtttgac
    32461 aaaggctttt ccacagggac caccattgta ggcgagatta tcaacggctg tggcatcgga
    32521 gaggcaggtg aagggtatct ctttgacaag gacaaataca agaatttcat gctcagactg
    32581 aaccaggcca tcggcaacag tgtctcaatc ggcttcttcg gatacaccgg gaaggaggtc
    32641 ataacggaca acgtgtctga tccttacgtt agtgagatga agatgtacgg acctgacctg
    32701 tcagtcgaca tagacggtaa actgctgctg aatgtacaat atttgtggag gactgactca
    32761 gatgtgtttg atggggacgg tgaggttcac ctgacagacg tgaaaacaca gggaggcttc
    32821 gcggaggtga tctatgcgcc taaaggcgat atgagcaaat ggtactttac gggacttgtc
    32881 aactgggttg actcagacct tgaccagctg gattaccagt cagggacgat acatgcgggg
    32941 tatctcctga ggcggaatgt aaggcttgtg accgaataca ccaggatctt cggcgggaat
    33001 ccctacggca agctcagcgc cggctttgtc tcggcattct gatcatgccg gaaatatgta
    33061 aggcgctggt gtaatgatgc cacggaccgg cagcaccacc gaacttttac cgtctttgga
    33121 atgttaattt ccaaatactg aatctatgaa aaaactcatg gtaattgctg ccgttgcatt
    33181 acttgcgtca tgcacggtaa aaaaggaggt cgaccagtat acgattgacc agttctacaa
    33241 gaatgtaaac acatggtttt cgggtttttc cccggacgaa acaaaaatcc tggtatcaag
    33301 caacgagaca ggaatctaca atgtattcga gatcagtctg ggtgacgggt caaagagaca
    33361 ggtcaccagt tcaaccgatg actcgtattt tgctgtcggg tatgttcacg gtacgggaca
    33421 gatcctctat tcagcggata aggggggaaa cgaaatcaat catatatacc tgctgggtga
    33481 ggacggcagc acggttgacc tgactccggg agatgaagta aaggcctcat tcaacggatt
    33541 cagcccggac aaaaaggtga tgtacttctc aaccaacggt cgtgatccgc agtactttga
    33601 tctatacagg atgaatgttg gtgagtggaa acccgagata atctgggaga acaccgaagg
    33661 atttgattat tccgggtcaa ccagggacag gaacattctt gcgctcgcaa aatcaattac
    33721 cacaagtgaa aacaggctct ttctttttga cagggttaca ggcaggacca ctgaaatatc
    33781 agaggcagac aagccgggtt catattccgc atcaggcttc tcgaacgata atgccagttt
    33841 ctactacact accgatgttg gaaaggaatt cgcctacctg gttaagtatg atattgccac
    33901 aggaggacgc gaagtagtat acgaggcgga ctgggatgtg aggggttgca gcttcagcag
    33961 gaatgaaaaa tacaggatga taatggtgaa tgaagatgcg cgtatcaaac ttaagattac
    34021 cgaccagtct accggccagg atgtagtctg gcctgatatc cagggatcca atatcgagga
    34081 cctggcaata tctgagagcg agaaatatct cctgctcgga cttggaacat cacgtactcc
    34141 cggggacctg tacctgtatg agttcggagg caccgatatg aagaagctca ctaataatct
    34201 gaatccggag atcaacccgg ccaacctcac tgaggcggaa gtggtacgtt atgcctcctt
    34261 tgacggtctg gagatacctg caatatatta taagccgctc actgcgacaa agaagaacaa
    34321 ggtccctgcc atggtgctgg tgcacggcgg cccgggcgga caggccatga cttactatga
    34381 cccgctgatc cagtaccttg taaatcacgg gtatgctgtc ctggatgtca acaacagggg
    34441 cagcagcggt tacggaaagt cattcttcaa gatggatgac aggaatcacg gcgataagga
    34501 cctcaaggac tgtatctggg gcaagaagtg gttgcagact cttgaataca ttgacagcga
    34561 gaatatcggc atcataggcg gcagttacgg cggcttcatg accatggcag caatgacatc
    34621 ggctccggat gaattcaaag ttggcgttaa cctcttcgga gtaaccaact ggcttcgcac
    34681 gctgaagtca ataccgccat ggtggggatc attccgcgag tcgttataca acgagatggg
    34741 tgatccgttc acggctgact cagtcaggct atacaatatt tcacccgtgt tccacggtca
    34801 gaatgtgaag catccggtta tggtgctgca gggcgccaat gaccccaggg tgctgcaggt
    34861 tgagtcggat gagatggttg aggccatcag gtctgcaggt gtgccggtgg agtatgtgat
    34921 atttcctgat gaaggtcacg gcttcgtaaa gaaggagaat gagatcaagg catattcaca
    34981 gatccgggaa ttcctcgaca aatacctgaa gggtacaggg gaggagagct gacaccaacc
    35041 aggtctcttc tgacggagta aattccaatg ataacaagaa aatggccggc ggtcagacat
    35101 gatccccggc cttttcttag ccagtgctgc tccttgtgtt acagcaggtc tgtggcttct
    35161 gcctttctgc cggtaaaggg ttcggtggca cggttgaata tgccatgaac ccaggctatt
    35221 gccatcccgt aatttgtaat cgggatgctg tgcttcttcg gctcgcggag cctgttcagc
    35281 agttgccggt gcgtcaccat gcaacctccg cattgaatga caagggcata ttcttcgatc
    35341 ggacgcggga aattatcgag cccggccaca ttgtcaaact caagattcct gccggtaaag
    35401 ttcgtcagcc agcgcgggat cttcaccctt ccgatgtcgt cacatgagac gttatgggtg
    35461 cacgactcca gcagcagcac ccggtctccg tccttcagct cacctatttt cggcgtacca
    35521 cgaagatatt ccgcgaagtc tcctttctgc cgggccagga tgatgctgaa acttgtaaga
    35581 ggtatgtcac gcgggatgga tgcgtcagcc ttgagaaaga cctggctgtc agttatggcc
    35641 agggccggcc tgatgccggt acggcggaga aaagcatcta cttccctctc cttcacgacg
    35701 atagccgtgc agtcgttatc aagtatgtcc cggataacct gcacctgtgg cagtatgagt
    35761 ctcccttccg gagcttcgat gtctatgggg gtgataagca gtacgatgtc acccggattc
    35821 acaaggtcac ctatcagccc gtggctttta agcgctgtgg cgggcatggc ttccctgatc
    35881 atcttcaaaa gtgccggatt gtacttttca gggtgcaatg cggagtattc cgcaacttcc
    35941 cggcctgtga ggctcatgac cttttcgcgc agttccgcgg ttaccgcggt gacatcactc
    36001 ttgttatgga taaccacgta cggcatgtca agtttgcgaa actcctcagt cagcctggct
    36061 tcgtgatcac cgaacctgtt atcggacaga acaaggatgg ccaggtcaat cagcttcagc
    36121 gatgccattg atttttttat tcgaagctca ccaaggtctc ccgtatcatc aatacctgcc
    36181 gtgtcaataa aaattagcgg tcccaggcca tgcacctcga tgctcttttt taccggatca
    36241 gttgttgtgc cggctacatc ggagactatt gctatatcct gcccgacgag ggcattgatg
    36301 agcgagctct tgccaacatt cctccggccg aatattccca catggggttt tgtatccctt
    36361 ccttttgctg tcatcttttt actcttcgat atccttcagg cggtatccgc cctggacaag
    36421 gttttcaggc ctgagtatgt cagagagttt ctccgggctg acatatccca gtttcttgtt
    36481 ggcctcggat atgcttaacc gttcttcctt catcaacgcg gccaatgcgg cagctttttt
    36541 ataccctatg aatggaagca gagcggtagt aacggccgga ctcgacctca cctgtttctc
    36601 tgcctcgcct gaatgcactt ccatccctga aagcaggttt tcagccattg agtggtctgc
    36661 tgctattagt gttttgatgc tttccagcag ggcatgccct gtcagcggaa gataggcatt
    36721 cagttccagg cagccctgtg ctgccagcga ggatacgatc tggtcattgc tgtacacccg
    36781 gtgtgagcag ctgataacaa actcggggat gacggggttg attttgcccg gcatgacaga
    36841 gctgccggcc tgtcttgccg gaatcgacac agaccttctg ccgtgcaggt cagatgccag
    36901 aatgcgcagg tctccagcca ttttctccat ggttacggca tgggctttta tgattccgtg
    36961 gatttccacc aggggatcaa ggtttgaagt ggcatcagag agattttctc ccctggtgac
    37021 aggaagtcct gtgagttgct gcaacctgtt aaccacttcg tttacataga acctgggaac
    37081 agtgatggat gttcccaccg ccgatcctcc gaggttaaca accttgattc gctcgaggca
    37141 tttggatacc ctccaccagt cgcgtgagag agcatcgctg tagctgccca ggagcctgcc
    37201 ccaggtggtg ggtaccgctt cctgcatctg ggtgtaacct atccgaagca ccgaacggta
    37261 ctttttctca agctcttcta tgtttttccg cagttcattt attgactctt cgaactccgt
    37321 gagcaggttc atggcagcca cccggagggc agtgggaacc acgtcatttg tagactggaa
    37381 tatgttggcg tcctcgatag ggtcaatgaa atcataatcg cccggatccc tgccgagaat
    37441 ctgaagagcc cggttggcga ttatctcatt caagttcatg tttatgcttg ttcctgcgcc
    37501 gcctgatatc gccggtacga tgaaatgtcc gaagtgtttc ccggccatgc actccccggc
    37561 agctgcggag agtgcaccga gtttttcggc ggttatgact ctgatattaa ttgcagcaag
    37621 gtcatattct tttgcggctt ccttaaaaaa tgaggcggcc gcaagatagc aggcctgttt
    37681 tgtcactgcc attgccctgt accactcctc attgaatctg ccctgatccg ggaagttatc
    37741 ccgtgccctg actgaatgaa tgccatacag tgcattttca tccagttctt tttctcccag
    37801 aaaatccttt tcgcggcgca tggtcttatt acttttcatc ttcggagaca ggaggtgaaa
    37861 gtttcttcga ctgggcttcc gcatcaattt cctcactctt tttctgcagc tcgtctatcg
    37921 agatgatctt ggctcgtttt tcggtcggag ggttcagttt ttccgatttt tcaaattcga
    37981 tgacctgttt tcggaggtct tccggcagat ctttctggct tttaaggacg atgtcaataa
    38041 attcttccga gaaaaactga tcgacctgta tgcggcgctt ggccgtatgg gcgaacttgc
    38101 cgattatccg gcagatgctg tcacggtcct ccgggctcag cttctctgcc aggtcatact
    38161 cctcgatctt tttaagcgac agtatcaccg gttccagggc atccctggtg atgacgaata
    38221 cgtcatagtc acccagccgg taagtcagct gctttatttt gtcgatggtg ttggtaggca
    38281 caaccaggaa catcgatttc ttgacattat cctggctggc gtatttctta aggttatcgg
    38341 cctggatttt gatgtaggtc gggaagttcg agaggtcgtc gtttgccggg ctcttggcgt
    38401 caaaaatgat atactcgccg ctgatttcga ttgtattgtc tggatttccc ctgaagggta
    38461 ccttttcaat gtagttcaca tggttgacat cgcagatccg gatgattgaa tttttaacgt
    38521 cagtctcatg ctctgaccac tgctttttca ttgcttcgag cctgtcagcc acttccctga
    38581 gtttctcctc gttcagtctt cgccgttcag cctcgaggct gtccttggtc tggttcagcc
    38641 gggcgatgtt ctcgttatag gttttaacgt tgtcttcccg tatggcttcg aaacctgcca
    38701 cttttttcgt tagctcctgt atgccggctg agtgttcccg caggcgtccg ttgagctcct
    38761 cgatgtatgc atccttgcgt gccgactgca cctccatctc acgcaccctc tgggagaggt
    38821 tttcgttgcg tgccgacagt tccgtgatgc ggttctgcag cctgcccgtg gtctccctga
    38881 cagcctctgt ctccctggcg agtgcccgga actcctcgta ggcatcaaag ctgaggttcc
    38941 ggactcttct ccagtggaag agcctctgcc agaagctgag atcctttatc cggtcaaaaa
    39001 agaggttgag tttttccatg aaggcaaagt tagcaaatct gtaatggtca tgtctgaagg
    39061 cagacaatta agttgcaacg cagcagagag cgcttttgcc ggtcatccgg gtttgcctga
    39121 gcggtgacaa ccctgttacc tgagcaatgc cgccgcgcac cagagcacgg gtgcctgtcc
    39181 gtggaagtcg ccggtgttgc gtttccggtt catatagtgg gagcggtcat cgctggttcc
    39241 ggtgccttcg caaacttcgg tcacatttcc ctcggcatca atatatttta ccagggagag
    39301 ccatgctttt ctggccgcag cgccgtatgt tttttcatcg agccagccgt tcttcacccc
    39361 ggtgatcatt gcgtaggtga acatggctgt ggatgaggtt tccttccaga actcaggatc
    39421 gtctattacc tgccgccaca tcccgtcctc tgcctggtga atgaggaggg ttgccatcat
    39481 tttcttatag ccttccataa tccgcggcct gtctgggtga tctacgggca tggcgcgaag
    39541 gatctcagac atgccggcgg ccatccaccc attaccgcgg ccccacgtga aatgggcgta
    39601 gggagcatga tagaagagcc cgtttccgag ctggatggta tccaggtaaa gtatcatttc
    39661 cccggcagcg cggtcaatgt acttaatgtc acctgtagcc ctgtatgcct gagactgcac
    39721 ggcggtgacc atgaacatgt catcaatcca gatgcgtgtc tgccaggtat agccaaggtc
    39781 agcataagcc ttctcctcag gtccggcatc ttccggcagt gtccattgtg tgtctgcata
    39841 gtacaggccc aggtcgagat attttttctc gccgttaatc aaatagaact ccagtggaac
    39901 tgatccgaag acattattgt caacgtgatt tggagggggc agcaggttgg tgatggtgtc
    39961 aaacagcggt tcaaaccggc tctgcagttt agtcagcagt tcactgttgc cggtggcctc
    40021 tgcaaaccat atgctgccga gccaggtgca gacctctgaa tagactatct gggttggggg
    40081 ctgaagggaa gagacatctc cgctgctctt gtgcggggtg gccaggtacc gttcagagag
    40141 aaggagtccc actttcccgg gatcagcgtc agccggccag cttttaagat cataatcagc
    40201 tgtctgtgtg caggcggata ttgccagaat cattgacaga ccgaaagcag aaagaacatt
    40261 tttcatcgga gcggaatcaa aggtattttt tcagtgaatt gaccgaatca aacggaataa
    40321 cattgtcaag ggacattttt ccggcaagga gtgcaagaat tccggcaaca agtattgtct
    40381 gatccctgaa ggcggaggag atgcgtttga gataatcctc cggcttgcca aagggcaggc
    40441 ttgtcagcgc accagtgacc aggaaatcag gatgccatcg ctcaacgaga tccttcaacc
    40501 cgttgaaagg ggttgactgg ccgaggtaca ggacactgtg acccgccttc cggattatgt
    40561 aggtgaagaa gaggagcccc atctcatgca attcactctc cggcaggaac attatgaatt
    40621 ttttacggtc aggccgctcc ggaggtgaga gggtatcgat ggcagagatc agccttttcc
    40681 tgaagatatt tgaaacgaaa tgttcagatc cgatatccac cgaacccgta tgccacataa
    40741 ttccgaccct tctgaggaag gggaaaacca cttcggtaaa ggtctcttcg aaacccttgt
    40801 tgatgatcga acgcatgagt atatcattga cccggtactc atcgaggtct gacatggcta
    40861 caatcagcgt gtcgagctgt gtttccgtgt cggacgattc ccttgtgagc agtttgacct
    40921 tttcttcaat ctcgtctccg ctgaaggttg cgatctttga tattttgaca cccttccggt
    40981 agaggattga gatattgagt attcttttaa gatcatcatt gtcataccat ctgcggttgg
    41041 tatctgtccg gtgaggcttg ataatgtggt accggcgttc ccagatcctg attgtaccgg
    41101 ccatgatgcc ggtgagcttt tccaggtcgc ttattgtgta acggtccatc tgatggtata
    41161 ttccgaggtt aaatatagtt cattgtcagt agtgttccat aatcacctgc aaaaaaagcg
    41221 acgccctgcc tgagcgtcgc cattttcatc gggtgatata ttgatgcctt acggcagaag
    41281 gacctggtca atcacatgga tgacaccatt ggaggcctgc acgtcagttg ccacaatttt
    41341 agtgcttccg ttgattgtgg gcaccgggct gagagccacg ctgattgctc cgttgagagt
    41401 ttcaacgttg cctgcctgaa gctgtgtcga gagaacgttt ccgctgacga catgataggt
    41461 gagtatcgga accaactgct cagcggtaag atcggcgaca ccggatattc caagggttgt
    41521 gaagagagac tcgaaagctg catttgtcgg tgcgaagatc gtgaacggac cggagccgtt
    41581 cagagtctcc accaggttgg ctttcacaac tgcctgtaca agtatgctga atgaatcatt
    41641 gttaagggct tgatctacaa ccgttggagg aagaagaacc ttgtcaataa cgtgtatcac
    41701 accattctta acatcaacat cggcggtaga gacattggcg gttttgttga gtttaacgcc
    41761 ggatgcaaca tcgaccttca ggctgagatt ggagccctgc gccgggctta acgttttata
    41821 atatccggaa gagatcattg atgattttgc ttccgttccc aatacatgat agagaagtat
    41881 aggggtgagg gtttccttgg tgagagcgtc aattccgctt atgccaagct ggttgaagag
    41941 agaggtaaag gctgcattcg tgggtgcaaa taccgtgaaa ttgcctgtac cgttgagtat
    42001 atttacaagg tcagccttgg tgagggcctg aaccagtatc gagaagttcg cgtcagaacc
    42061 agcgtattct gctattgtca tatcagatac cggcttcatt tcttcttctg ccttctcgca
    42121 acctgtaagt ataaagattc ccatcagggc aatcattccg taaataagtg tctgcttttt
    42181 catgatgttc ggttttattg gttattgaat tttgaacata gaacatcatg ggataattaa
    42241 tgttcaagaa aaaatgtaaa atgatataca aaaatacata ctaattctag aaatgaatca
    42301 actaaaactg tgatatacag taaattgata gtgaaatgtt aaaatatgtt aaatgtataa
    42361 gaaaaaggaa gaataagtat acataataaa acaatgacct tccggtgacg ttgtgggtga
    42421 gactattctt tggtccagat gcgggtattg aggttgagat catcaaccac ttccgtaagc
    42481 agcgtcagga accggagggt ttcttccagg aaatcatagt cggccgcagg cttgaacggc
    42541 cggagttcga agaggttcct gttcatgctt atggcaatac agaccttcga gtcgaggaaa
    42601 gagaggtgta cctttgtatt ccacttctcc ctgaaggcaa gtatacgttc catcagggaa
    42661 ggtgtgagga tataccttga ctccacctgg tcatcgccgt agacgcagaa ctccttttcg
    42721 aaccgggggt cttccaggcg aatcagttcg cccctgccga atgaaacaga ctggagagac
    42781 tgtccgaatt tgccgaagat cttttcggca gtgtccggaa gcacaacagt atgtgttttg
    42841 aaattcttat tgaaatctgc gataatgaat attcctttga atatggttgt atactcatcc
    42901 ctctggtggc ttcccgcacc gctggtgctc cggtgccttg cattgacttc ggagaactct
    42961 atctcagtct tgcctatcct gccgcggaaa taatcctctc cgctgtactt gtcgcatgac
    43021 agtgtgaata tcctgcttct tatgaaatca tccatggtga tacaggcgtc aggctggtat
    43081 gtaagtcctt cgtctgcata tcctgcgatg cgtgctatta tcttttcctt gtactccttc
    43141 cggtagtttt tgctgacaat accggtagca accagtcccg cgaaggcggt gatgattatc
    43201 ggtgccggct tcgggaaagc cgtgttgccg ggtatgagca tcagctctgc cagcacggca
    43261 gcggccgtaa taagcagggc ggtaagggcc tttttattgg ttgccctgcg tcgcagatca
    43321 attgattcga ggtcggggcg cagttccttt tcgtaaaatt ccctgaaagt gccgtatctg
    43381 ctcatccttc ttttgtgttt tatgccatat tcacaggcgg tttggagctg acctcttccc
    43441 gttttgctgc ggggatctca aagaaagatc ttctcttata ttgcatcatg gaggcaatga
    43501 tatttgaggg gaagatctca atgctgttgt tatatattgt aacggcagca ttgaatgcgc
    43561 ggcgtgatgc tgcaagctgc tcctccacct cgttgagggt gccctgcaac tgcaggaagt
    43621 tggtgcttgc cttaaggtca gggtaattct ccactgcaac cagcaggtcg gtcagcctgg
    43681 cactgaggcg gttttccacc tgcaccctct cctctgccgt gagtgtttgt ggcagtgcct
    43741 tgatcctcag ttcggtaatc tcggtgagga gctctctttc ataattcatg taagccttga
    43801 cggtttcaac gatcttcggg ataagatcat accttttcct gagcatgacg tcaatggagg
    43861 caaatgaatt ctccacatcg ttctttctcc ggataaggga gttgaagatc agcaatgccg
    43921 aaagcagcag tataaacgct gccgcgataa gtacatatat cattgcgggt gtgttaacag
    43981 gttcagcatg attcagcccg gtcaatgtgg cgggcttgta aaaatagcta ttttaatctt
    44041 ttcagaggaa caaaccgcac cggctcgagc cgctgttcgg ttatcctgcc ctttcttttg
    44101 gtcagcacaa taagctcctg aactgttgcc gggtttccga ccggcatcac cagccgtcct
    44161 ccctctgcca gttgttcctt caggggaccg ggtatatggt cagccgcggc agtcaccagt
    44221 attatgtcaa agggcgagtg ttcaggccaa cccttgtatc cgtcgccgta cctggcaact
    44281 atattgccgt aacccagtac ttttagccgt gaagctgctt ccctggccag ttccggtaca
    44341 atctcaaccg tatagaccgt atcaacaatt tcggccagca cggcagcctg gtaccccgag
    44401 cctgttccta tctccaaggc ctttttacct ttggccggct ttgacagttc agtcatatag
    44461 gcaacaataa aaggttgtga tatggtctgg ttgtaaccta tcggcagtgg ctggtcactg
    44521 tatgctgaac ctgcgagtgc cacaggcacg aacaggtggc gcgggacccg ttccatggct
    44581 ctcagcgtgg cagggtcact gatgccgcgt gcgactatct ggctcttcac catctgactg
    44641 cgggcccggg tgttgcgtac ctcttcctgg tccgatggca ggaagataac gagggttaat
    44701 attatcagca ggctcttcat ctggttccga ctgatacaag ttacaaaaaa atgtccggac
    44761 agtgtatgcc ccgggaaagt acgggtctga tctgggcata tcaataccct gctctccggc
    44821 atattttatt tttcttcttt ttcttaaatt catgacatga aaaaactggt catcttaaca
    44881 ggagccggaa tgagtgccga gagcggcatt aaaacattcc gtgacagcgg aggtttgtgg
    44941 gagcagtaca aggttgagga ggtggcatcc atcgaaggat ggtaccgtaa tccggacctg
    45001 gttcaaaagt tttacaatga tcgccggcgc cagttggaga aggctgttcc gaacgctggc
    45061 catacgggcc tgaaagagct tgaaaagctg tttgatgtgc atattatcac acagaacgtc
    45121 gataacctcc atgagagggc aggcagcaaa agcgtgctcc atcttcacgg tttgctgaca
    45181 gaggcgcgca gcagcaagaa ccctgacagt gtaattgata ttggctatcg cgagattctc
    45241 ccgggtgaaa aggctgctga cgggtcatcg ctgcgtccga acatagtctg gtttggtgag
    45301 gctgttcctg ccatggaggc agcggctgac atcgcctcac aggctgagct atttgccgtg
    45361 attggcacat cgctgaatgt ttacccggct gccggcctga tacattatgt tccggcaagc
    45421 ataccggttt tcctgattga tccgaagccg gttacagttt acacgaacca caaggtcgat
    45481 tatatttccg agggtgcggg caagggtgtt gccattctgg ccggaaaact gatggttttc
    45541 gccgcttaag gcggcatgcc ggtccggata taactttttt taactatccg gtatcataat
    45601 aaggtgctgt tatccttccg tttcttaacc ttaatttata gtttggcggt actaaaaatc
    45661 caaaaggagt gacatgaaaa aactatttct tcccctattg ctgatattgc ttgccacagg
    45721 aatgcaggcc cagcagaaca aggttgttga ccggatcatt gagcttggcc agacagacaa
    45781 ccgtacgatg catcatcttg atgtgctttc aaaccgtttc ggcggaaggc ttgtcggttc
    45841 cgatgcatat gagaatgcag ctgattggtg tgcatcgcaa ttcagaagct ggggcatgga
    45901 ggtgatcatg gacgaagccg gaacagttcc ggtaggattc aaccgtggtc cctggttcgg
    45961 caggatgctc tctgacaacg gaatgacact gcattttgcc actccttcat atacagccgg
    46021 aacaaagggt gttcagcgcg gtcatgttct cctcgagccc aaaagcaagg atgagtttga
    46081 aaggatgaag ggagttctga agggagcttg ggtgcttatc ggcgggaaga atgacggctg
    46141 gcccatcgac tggtcgccca ggggcgacag cataagggct gcagccatgg tttacaatga
    46201 ctccatctcg aggcttaaca gggagatcat gcgtgagaac atgatgaaca gggagaatcc
    46261 cccgaaggaa ccccataagc tcagggaggt gcctgctctc ttctaccgtg agatgtgcga
    46321 ggcaggaatc cttggcgtga ttcagtcttc ggaagtaccc atcagggcgc tttatgacag
    46381 gaagaacctc gacaggatga catttgacaa cctcccgacg gtttgcgaca tcaagcttga
    46441 tgagcaccag tatgatttaa ttgcccagat ggctcgtgag aggagatatt ttctcctcga
    46501 gtttgatata agaaaccact tcaaacccgg accggtgaag tactacagtg tgatcggcgt
    46561 aatcaagggt aagaaatacc cgaacgagta tgtgatgatg ggcggacacc tcgatgcatt
    46621 tgatgttgca accggtggtg tcgacgacgg atcaggcgcc agcccgacca tggaggctgc
    46681 acgcctgata atggctgccg gcggcaagcc tgaccgcacc atcctggcct gcctctgggc
    46741 tgccgaggag ttcgggctgg ttggctcaaa acactgggtt gaaaccaaca aggacaaact
    46801 accgaagatc tccaactact tcaaccgtga cgggggaccg atgccaaaca ccgggattac
    46861 cgtcacaccg gccatgtacg atgattttga aaagatatgc aaaccgctga acagcatcaa
    46921 tcccgacttc cccttcacac ttaagaagcg cactgatcag ggccggcccc gtccgacctc
    46981 ggcaggcggt tccgatcatg catattttgc aatgaacggc gttccaacgc ttaatttcga
    47041 cggtccggat gtcaagggtt acaacttcaa ttacggagag atatggcata ctgaacgcga
    47101 cacatatgac aagagcatcc cggagtacca ggaacatact tcagtggtaa ctgctgtagt
    47161 agtgtacggc cttgccaatc ttgaccacct gctgtcaagg gaagggcttt acagggaaga
    47221 gaaatagtca ataatttaaa taaggggggg gtgatgaagc atggattggc ttttgctgct
    47281 gttgcattgc ttgctgcagt gattgtagct ttcggccagg caagaaaggt tgaccagtct
    47341 gtatatgtgc cggcagatcc gcaggtggtg caggatcagg atgagatgac ctgggatgac
    47401 tacaggccga tccccggtat gaactgggct gacccgtccc tgaagccgga gcgtgagttc
    47461 cggatggccc ttgtggctgt tgacttttct gaccagcctt ttgtgatgac cctgcccaaa
    47521 ggatcagacc cgttcgggaa tccgcagatt gatccggtag accgaagtga gataccgcag
    47581 ttttatactg atttctggct taagccttcc gctgtcaatc atggtcagac cattaacgga
    47641 tactggatgg agcagtcgag gggaaagttc ggaatcacgc agcttgaagc cttcggccca
    47701 taccgaatgc caaagccgct gtgggcttac gggctgagtg agtgggggca aaacaatgcc
    47761 acgccggacg gatccagtgt gcgtaaccgg atggagcacg atattgactc tctctggcat
    47821 gccgacaaag gtgacataaa aggtaattat gacgctatcc tgagagtata tgcagggtat
    47881 gatgagacag gggtctggca ggagtttggt gagatgaagt tcaacagcag ggatgagatt
    47941 ccggctgagt ggggcaatcc aaatcctgac aagccacgct gggctcccac acggtatgtt
    48001 gaatggactt cctggaaggc aggccagatg caatgggggc tttcatccat ccggcagggt
    48061 gagaattcag gcaccatcac ccatgagctt gggcattttg ccttcagcat aggtgacaat
    48121 aacaataatc cgtatgtcaa accttacagg cgagccggat cagggccatg ggatatgatg
    48181 gaccgcggtt cattcaatgg tcccggcggc cctcatatga ggtgggtggt gccacctgtt
    48241 gcgggggcag ctatgccggc aggtctgatg ctgcgcaaca ggcttgtgaa caggtttatt
    48301 accgaacatg atctcctcac cgtgagccgg gaaggactgg cttcttcggg ccctgtggtt
    48361 gctcggatca cggcacgcgc cgttaagccc ctgccgggcg aatatgccgg cataatggtc
    48421 aggctcgacg gtgctgagcc tcatgaccgt acacctgtga cagatccggc ttcagacccg
    48481 ctctctccgg gcaggcccag gttcaattac tactccctcg aagtggtgca gcgcatcggg
    48541 tatgactcat tctgccctga taacggggtg ttgcttgcaa tgaacaccga cggagaggga
    48601 ggcaatggcg gacttaacgg attcaattgc tttaactggg taattgatgc tcaccccgag
    48661 gatatcaata tggtggattt tgttaagcct gatggtgaaa aggtgatgcg tacggtggct
    48721 gactaccggc agcttaatga tgcacttttc catgcagggc tgaattcagg cagcaagttt
    48781 gaatatgttg acacccacaa ccggctccat ttctatgtca ttgacattca tagaaatgaa
    48841 cagggaattc tgtcatacac agccggggtg cggtcacttg attctgatgt tgttaaggaa
    48901 ccggccacgg tctctgccgg gaaggttgct cagaagatcg ccggcgaagg aattgttgag
    48961 ttcgaggttt caggagatga tctgtgcagg ttgcacgccg gtgtcacggg tgacggatgg
    49021 gaggtgaaac tatttaatga ggttgttgcc cccggtgacg ggagaaccgt gaagattccc
    49081 gtttatgtca gggcaaaaca aggctgcagc aggagggctg aaatcaccct ttccgctgtg
    49141 tcggagagcg acagcaaata caggtcagca gcctcgagaa aggtgatact gcaatagctt
    49201 acccggtttt tgcgcggaat taatttttga acatcctgat ttgtggcttc gttcaggtat
    49261 atttgatatc tgaaaggagt ttgatgtgaa ctgtttgctg accggatcga acggatttat
    49321 tggcaggagg ctggttgagg agctctccgc cggcgggaat aaggtgaagt gcctggtgcg
    49381 gtcacctgaa aagttcaggg aactgtcctc ccttcccggg gtgactccga ttgtcggaga
    49441 ccttgatgca gtggatgtac ttgaggaagc ttcggcatgt tgtgacacgg tttttcacct
    49501 tgctgcgtat gccaaaccct ggtcaaagga caggagcctt ccgtacagga tcaatgttgc
    49561 cggaacggag aacgtgctaa gggctgcccg gaaagcaggt gtcaggcgtt ttgtcttcac
    49621 ctcttcggct gcggtgatag ggccttcgcc gggaaccgaa cccatagatg aatcattcct
    49681 gcgtacagtg ccatggttca atgagtacga ggagaccaag gcagctgccg aagagctggt
    49741 gaggtcatac aaccgcgacg gaatggagtg tgtaattgta aatccgacaa gagtttacgg
    49801 tccgggacca atcaatgaga gcaactcggt cacgaagatg atttctcttt atgcacgggg
    49861 gagatggcgc atcattccgg gtgacggcaa atgtgtgggc aactatgtaa tggttgatga
    49921 cgttgtcagg gggcatatac tggctgctca gaagggcagg cctggtgagc aatacatact
    49981 gggcggagag aacctgacct ttgacacgtt ttttgatacc cttgccaccc ttaccggcag
    50041 gcgcctgtgg ttggtaaaga tgcctgtcag cctgatgtcg gcagcagccg gggtgatgga
    50101 atggcaggca tcatttacgg gcataccgcc gctgataact ccgtcatgga ttaagaaata
    50161 cctgaatcac tggagcctta gcagccgtaa ggcgacagag gatatgggct atcagccaag
    50221 gcccttcgcc gaaggcgcaa agattacaat ggaatggctg cagagtggct ttgaaaggta
    50281 aaatgttaag atgagtcagt tgaaaggagt catccccgga tccgggataa ctacctgatc
    50341 acaagttttg tgaatgctgt cttatggccg ttgccgcatt tgacaatgta agttcccggg
    50401 cgaagcttaa catcactttt ttgttcaaca cttgtactcc tgcttgtcac ttccatgatc
    50461 ttaaccgtga tgccggatac gctgcagata gacatcaggc aaggcaaatt aatgcattcc
    50521 ggtatttcaa ccgtgaaatt gccggatgcc ggattagggt atacattcaa tgccagggct
    50581 ggtgcattta ttgacccggc tgaagtactt cctgataatg tcattttcca gagttcatcc
    50641 cccgtggaag gtattgctcc attaaagaca aatgttttgt ttatacgcct gaattcaggc
    50701 ccccaggcgc ctccgttcgc aacattggta aatttttcag gttgtccgtc cggactgagc
    50761 aaacgccata tttctccgtc tgttccgtca tttgcggaaa aataaagtgt gtctcctata
    50821 atgaccgggc tgacaactcc cgaacccatt attcccggcc agatgtcctt tataattttc
    50881 gttcctgatt ctgttccgtc agaaacccag agttcgactc cggttgaagt tactccggca
    50941 gaaaatacaa gtttattttt gaatcccgtg aaaaatcccc ctgataagtt accgtgaccg
    51001 tcagggttta tatccctcac catatgaatt ccgttatcat cataaaccca taattctctt
    51061 ccgtagggtg tcttttcagc ctcaaaaaac attttgttgc cgacaacagt aatatgtctc
    51121 ggatcgaatt gtgttgttcc gtcaacaata tcagtaaccc tgatcacgcc gtttccggat
    51181 accttccaga gttgtgaccc tgttccgctt atctggtctt tagcacggaa atacatttcg
    51241 tctttgaatt ccgtgaaata agcctcatca agttgcccca ccctgatctg gtcggaaatt
    51301 agtatcgtac ctccgggagt tccgtcggag aaatagagtt tcaggcttga caatccgttg
    51361 aagtaaagtc cgcagctgcc tgtggttatg tacccgggtt gcgtcgaagc agcaccttca
    51421 gtcaggtctt ttaccaggag ggttgtctca gtggttcctt ctgtcttcca caattctgtc
    51481 cccgtatcag ggtgataggc actgaaataa agaacattat tccattcgat gagatgtccc
    51541 ggtttacttc ctgtgcttcc aggtgctatg tcctttacca tgatggttcc ctcagtggtg
    51601 ccgttggttt tccatagttc tgaaccatgg gtgccgtcat cggcgctgaa atatgcctca
    51661 tccttataaa ctaccatgcg gccgatactg cttccggttg tggttgaagg gtttatctcc
    51721 ttcagcagga aggttccatc agtggttccg tcggtgaccc acagctcgtt attagtgttt
    51781 ctgtggttgg ttgccttgaa aaccaccttg tccggaagct gcgtcatata gatgataaat
    51841 gaactttcgc tgccggcgtt aatatccttc agcaattcag cctgctgtcc tgccatggtg
    51901 caagagagag cagccaggat aaatggtgat atcacttttt tcatgggatt atctgtttta
    51961 gtttatgagt gctaaacctg tcagagtgct ttgagatccc atctgactat ataatgatag
    52021 tctgtcccgt ccttttccgg gtcaaagggt atttgctcat atatggcctc ggtcatgtct
    52081 gtaattctga caccggtagc ttctttggag gagctccaag acacgtcccc gtgcagatgg
    52141 ttgatgtcct ttaccttaaa tctggcatct gctccgataa ggttgtcggt ggaggttgag
    52201 ttgtcccaga cgcatccgac atccttaaca tagtgtcttg atcttgtctc cgtgggcaca
    52261 ttctcggtgg gtgtggcaat gtcataccag ttttccagtc cgaactggcc cagctggtca
    52321 gcaacaactt tattatcggt tgggaacagg tttgttatca gtatcagacc ctttattgat
    52381 tctcggggaa aatctcctga gccaaactcc tgatcgacca gtccggtgca atcgcagtcg
    52441 ccgctgcagt aacggtcttc tttgtggctc caggtgcctg taactttgtc gggtccgaat
    52501 attgtgattg gctggccctc cattccctcc atctgcggga ttggcttgaa tatcccggtg
    52561 aaagagactg tttgcgaccc agaatatctt gtcgaactct tgccgtgatc ttttgtatag
    52621 tcccatctct tcgaaacagt tatggttccc ttgtagaatc cgcagaagat cgggaaatgc
    52681 tgatcaacaa taagttcatc cggagctgtg gcggacaacg ggtgtttatc ctttggcagg
    52741 atatcgcatg aattataaac tctcagccat tcataaccgt catccttatc ttcaggaggc
    52801 tggtactcga ctctgacctc accttcccct acattaaata ccttgtaatc aggcccataa
    52861 tcgctatcag cgccattgag gatttttcct ctgtagatgc tgaccacaat cctgttgaat
    52921 tcccgtgacg gcctgccgcc aacgtcttta aatcctgaca ggataattat ccctttccct
    52981 tgttcacaga tctcctcctg ttctggcact tcaacgtcaa ttgagacagg tttcttttca
    53041 aattcttcga agatcgggtt gagagatttt ggcgttttaa gcttattaaa gagtggccgt
    53101 ggatcattga ttgtattctc tgatgaccat tcctgaatta gttcaggggt atttccgtta
    53161 tagtaaaggc gaagttccat ttttgctatt acgggcttct tataatcctc ttcatactca
    53221 ataatgcgac cccctttaag atttgcctcg taacttccct tgaagatgta ttccggcggc
    53281 ttgtctgttt cgggggcata aaagatcggg gacaaaaaat ggaagcaggg attggtaaat
    53341 ctttcaccca tgtaaactga catcattgta gggtagcaat accatttttc aggcggatca
    53401 agcgttttgc acttatcaat tgcatcagtc agttccgggt tgagcgaatc ttcttcccgg
    53461 aattcacgat ctccaagctg ctctgtcatg catgcattcc attcctttaa cctttgttca
    53521 tagtcctggt tatgggggtt caggaactct acaagcattc catgccttgc gcattcttca
    53581 catctgcgct gggcacctgc ctggaagccg actatcagga tcagcaacga tgtggtcaga
    53641 accctttttg aataagtcag ggtggtcatg aattaaattc tttcgaattc gacgtcttcc
    53701 cagtcgtcgt ctgtatcagt gtcattgttt atgtcacgaa ccagccacag gtagtcatca
    53761 tctgcttcga acgtgccttc aaatgactct gacattccta gtgaaaggta tgcattatag
    53821 gttgctgatc ctttcgggaa ccactccggc gattcggtgc aattgtcatt tgtgtcttca
    53881 aggcagtctc ctaatgcagt aagcctgaac agcacttcgt cgccatcgac tgtgatattc
    53941 cctttcagcc cgagaagtga cataaccgca gagctgttat cacgtcggta gattacaacc
    54001 tcctcaaatg tgccttcagc aagcttcagg gtgtgtgtta tgtttattgc aacctccccg
    54061 gcatatccct tttcagtgta ttgccaggta ccgacatagt caggatcggt tgtgaaccaa
    54121 tcgcacgagt gaaagcagct caccgtgaaa agaagggtca gtgagagcat gattttgttc
    54181 gacaatattt tcttcatggt atagtattta tggtttttat atgataatag cctggcctta
    54241 tcggccggcg cattttcaat acagttcagc agttgcctgg attgtggtta cctcaaatcc
    54301 ggcactcagg aatccggttc caactccaaa tccctcctca gtttcaattg ttaattttcc
    54361 ttcatcaatt ctgacgtaga cggcctcctc catgcccata aagtcaactc cggcgccaag
    54421 ctgaaatccg aaacctgtct ccggtttcca ggtaacacct acttcaatcc ccgcgcctat
    54481 attgaattcc cactcattgt tatcagtatt ccattcaata ctgacaataa agaggtccag
    54541 gctgacggta tgttccaccg gaaactcagg tttcctggtg tctccgccct ccccgtcagg
    54601 acaataatcc ttgatgttga agcactccag ggggatcacc atgaagagga gtaaaaagtc
    54661 ctgtgggtca atggcatcta tattcgattt tggaagaccg tatgggctgg cttcggcggt
    54721 cagccagcgg tatgcctggt ctacctggga tttataacag taatttattg cctcctccag
    54781 ggcttcctgg tatgcttctg cccttaattc tgccatactt tcggctgtca atggcatatc
    54841 tctccagctg tttatctgct cattggcaga aatccttgcc tgctgcagtg cgcattccat
    54901 aaaaaaggag ctgtatccca gcagttcaga tcccatctga cgggtcagtg atgcccttct
    54961 gtgcccggcc tccatcatgg aattaaatgt caagcgtgtc gaggttctca ttactaccgg
    55021 tatgccggat atatacctgt tccagtcttc aggcatgttt ttccacctgg gccagaagtc
    55081 cattattacg ccctcaactc gctttgacag ggcatcagaa taactctggg caatgttctg
    55141 catctgttcg tcatatcttc tctccagcaa ttcaggaatt gtccgggcct gctcacaagg
    55201 ggacatctgg tcccacgggg tcaccatctc gtcttctgta aatccctctt cctggagctc
    55261 ctttctttca atctcgatct gttgtttgaa ttcttcagga tcaagtgact ttttcacggg
    55321 tggttcatga ggtggttttg ttgtatacca gaacagcagt ttttttgcgt cggcgggatc
    55381 gagttcggaa gcctccctaa actcttcaaa tgcttctctc ttcattccca tccggaggag
    55441 tatgatgccc aggttcatat gatattgtgc tattccgggc gtgtagaaga cagcttcacg
    55501 gaaatatttc agggcttcct cattctggcc gtggcgtgcg aatacccatc cgagagaggt
    55561 agctgtgggg gctgattcgc ggaagaggct cctggcgtat gcaagcagca ggagcgcttc
    55621 agcgtccctg tctgaaacag ccaggcagaa tgcaaggctg ccgattgacc cggcagatct
    55681 ttcatttgtc tgaaatgccc tgccatagcc ccatgaagca gcatcaagat tctgctgggc
    55741 caagcctgac attcctgcca gctggtacac aaagaccctc tcatcaaatg aaagaatctt
    55801 cccctgaggc actgatctga aagcctgctg ctcaatatca ggaggcaggt tcgggatggc
    55861 atgcatcacg cactctgcga caatctttcg taccgtactg atgccggggg cctcgggaac
    55921 cgagaccggg tcaaccggtt caaaatcttc atcattgaca atcctgtcgc aactccggtc
    55981 catgacagaa agttcaagga acagggccag ggcatcctcc acatccttcc tgtcatcccc
    56041 gttaagatcg gtttcaatgt tcagaccacc cctgtcaata aatccgtcaa tatcgcttct
    56101 gtcgaactga ccgtcctggt ttatatcata ctgccgccat tgtttcgtgc ccggggcatg
    56161 agcactcccg ggttcagaag catatgataa agacgcggca cagatggcct ggacaagcag
    56221 cagtgccgca atatgtctgc aattagtaca catctgtcag atctttatga atttgctata
    56281 cacattcgct cggcgcccgg cagagcttca ggcaagcctg gctctgcgtc acctctttac
    56341 gaattcgact ctgcggttag ccgcttttcc ttcggaggta ttattgggcc cggagggctt
    56401 gctttcaccc cagcccctgc tggtcatacg tgagttgttg atgcccatct ttgcaagctg
    56461 gtcggcaact gccttcgccc tgcgttcgga aaggtcctga ttgaatgcct cttcaccgtc
    56521 gctgtcagtg tgcccctcga ctgaaaaact gatttcagga tgatctttca tcagtttggc
    56581 tatttcgttg atgacaccca tggactccgg ttttattgta gccttgttca catcgaaccg
    56641 gatgccactg gcaactattt tgccgtcctg catcagcttg tcataaagcc tgacagctcc
    56701 ttcggctatc ctgatgtttt taatgaacct gttatttccc tgtgatccca cggtgttcat
    56761 cgggtcacag catattgtca gaccctccgg attgattccc aggttgggaa cattgaccac
    56821 tctggcatca tcaaggtaca ccttcagtgc ccttgtattg aagctgattg ccacatggcg
    56881 ccagtatcca ctggtgatct cacctttctc ctcatacccg ggataataac cttcgcccac
    56941 atcattaatt ccggcatgat tggcgttgat aatcagcgga tcaatgctta tcgtgctttc
    57001 actctggttc ttatgatccc agaagtcgac ataatactgg caatattcat cgggttcaaa
    57061 ccagcagtcg aactccaccg taaacaggtc tggcaggtta tcttttgccg tttccctgaa
    57121 aaacggaatg atgcatgaac cggcttccct gaaatggatc acattctggt caccgaagga
    57181 cgcgttttcg acatttcctc ctcctgcaag atcccacctg gatgggaact ccccgttttc
    57241 ctctccctcc tggttatcct cgaagattat cttctcaccc gggacaaagt catacctgtt
    57301 ccatttaaaa gtgagctgtg gctgacctga ttcctttgct tcagtggggt ttctcccgga
    57361 agtattcccg ggatctgcgg attcttctcc tgcttccttt tgagatgagt cttccttcgc
    57421 ctttttctcc ttctttttga aaatatttcc aattccttcc tctatcttgt caaacccggc
    57481 gtcaataacc tcgtcagtct tccggtttgc gcggtggtca gtctcctcca caaccttttc
    57541 ttttacattg accctgactt tctgcgggct ggcttcctgc ggcagcagaa agagcaatga
    57601 agccacaagg gtaaataaca ttgagtgttt catggccttt tggttttttc aatgaaagtt
    57661 agtcaggcgg ccggggcaag tcaacactga atgagcaaag aggggtgatg aatgagtatc
    57721 cggggtattt gggatgtgcg atttgcgatt tgcgatttgc gaattgggat tgctgatacc
    57781 ggctgcctca ggatggaggg gatgtaaaaa ggggggatgc gaagctttat tcgtaaaggt
    57841 gatcgttgag ggactgcaga accttttgct tcaggacagg ggagaccggg atgatggtac
    57901 cgtctttaag ctgcagataa ccaccatcat gcctgtaaaa cttatcgatg taattaaggt
    57961 tcactatgtg tgactggtgg accctgataa ggcttgtgcc gctgagcatt gcctcatatt
    58021 cctttatggt gcgtgagaca atgatatgct ttccgcctgc aaggtgaaag tgtgtgtaat
    58081 tactttccgc ctcagccctt attatatcat ttactgaaat cagttgcagg gcttcagctg
    58141 tcctgaggat gatccgtttc aggacttttt tgccctgcag gttttctctg agagcgagca
    58201 gcttcagttg ctcctcttca tggttaataa tttcacgtgc ctttaagatg gcagctctga
    58261 gttcatcctc atcaaccggc ttaagaatat aatcgagtgc gctgaccttg attgcatcga
    58321 gggcgtactc ctggtgaccg gtgatgaaga ttgtacggaa ccggaccggg gagacctgtc
    58381 tgagcagttc aaagccgaga ccgtccggca gttcgatatc caggaagacg atatcaggct
    58441 gatgttctgt cacagcggca ataccttctg ccactgaccc tgtggcagcg ctgacggtga
    58501 tgtcaggaaa gctctctttc agaagggttg caataatact tcttaccggg gcttcatcat
    58561 caacaatcac tgctctcatg gcatgaaaca ttttatgcgt aaatcttctt ccatggcagc
    58621 atcatcacca ccctggtgcc tgcggcattg ccatcctcgt acaggtcaat gatctcgtaa
    58681 cttatctgtt tctccttcag gatttttctg aagtgttcca gcctcttttc agtcagtctt
    58741 gtagagatgg attccttttt cattaaaggc ttatgctctg ccgccttttc cctgcctatt
    58801 ccattgtcag ccacttccag catcatcagt ccgttcctga gagaatacct tacgcttatc
    58861 cggccgtctt tcgtgccggg cagaagtccg tgttcgatag agttctcaac acagggctgc
    58921 gcaagcatgg gaggcactga aacattctca gggtctatgg cttcgtcaat ttcaatgccg
    58981 taactgaatc cgttttcaaa cctgagctgc tgtacgtcca tgtaaagttt aagagtctct
    59041 atttctttat cgagtgcgat atactcctcc cgggtgttct ccaggatact tcgcatcagc
    59101 tgtgcgatct tggtaagaaa agcattggct ttctgtggtt tgtccttcag gatcagatcc
    59161 tggatggcgc agagcgaatt gaagatgaag tggggattca tctgggcgcg aagcagcctt
    59221 tgttcaaggt ctacctgtgt atacctggcc ttcaggcggg catgcctgat atagagcaat
    59281 gccaggattg cgattattgc cagcaacagg acaaggccgg aaagaagaat cctctgttgc
    59341 ctgatcttgg tttcactcaa gaacctttcc tgcatccttt caaattctgc cgctttctcc
    59401 ttcttgtcaa aatcatactg gatctccatt ctcgtcacct gcttaaggaa ctcgtcattg
    59461 ctgatcgagt cacttagctg catatgctct ttcaggtacc ggtaggcctt atcttccata
    59521 cctcttttgc tgcagatatc gctcagcagc agcgtcgcgt cggagacaac cagcggaagt
    59581 tccttctctt ttcccagcct gtatgcggtg aggctgtttg agtaagccct gtcatagttg
    59641 cccagagttt gccagatgtt accaagggtg ttataaaact ccggcggcac aggcagatct
    59701 gatgacgatg taatgtccat agccattttc atgtaattca gggcggagtc aggctgtgaa
    59761 aggagaagca gtgtggatgc catgttatgg caggcagaag cttctcccac cggaatatgc
    59821 atcaacctgt taagcacaag gcttttacgc tgatagacca gtgcactgtc aaacgcctcc
    59881 cgtgagttgt atatctgagc aatggtattg catatcttgc tcacctccca taagtccttc
    59941 cgcttttctg agagcatcag gtgttttctg ctgaattcca gtgccttgtc ataatttttc
    60001 tgataataat aagccagtcc gatacttccg taagctattg acattcctga ggtgtccctc
    60061 tttgcttccc agagtctgag agcttcaaaa taagcgtcga tggcctgact gaagagggcc
    60121 atttccttat aaagattacc caggttgttt gtggcgtctg caaggggcag tgtgtctttt
    60181 acctgacttg ctatggcaat tgctttctcg agataacctt ttgctgcttc cagatcctga
    60241 ttctgcctgg ccaggtaagc cagcacattc aggctgttga ccatgccgcg gtgatccttt
    60301 gattcttcga agtatttaag agccaggttg atgtaatgtt cggaagatgc atgatcactt
    60361 ctgaaactgt aaacatatga cagaccataa cacgctcgtg ccttgccggt tatattaccg
    60421 gaggcttcat agagctccaa tgcccttgag ttataataga gtgcactgtc acccggattg
    60481 tattttgcga ggtaagccat cccgagtgca agggatgcat cagccattcc tttgatataa
    60541 ttcagctttc gcgaagcagt caatgcctgg tgtgatatca tgattgcggt gtcaggattt
    60601 ctccgggcca tggtataagc ctcttcggca gttttagtta tggctgccgt gtcagccagt
    60661 tcctgggaaa ataaatgacc ggccggaatc accagcaata aaaccaagag tcctgctaag
    60721 gttttataac aagttccagg tgacatattg gtacttcccg gttaacacca gagaactaat
    60781 ttcgtaaaaa aagcccttca ggtcaactgt ttcagaggga aataatatca attctctggt
    60841 caagcatgtc tgcctgcagc aggcggccgg acagtatatc gccggcaccg gtgattatct
    60901 tgatcacctc gcatgcctgc actgccccga ttatgccggg aagggggccc atcacacccg
    60961 gaggacctga tctgtcttcc gtcttcaggt gcatctcctc ggggtagaga tcgcggtaag
    61021 agggcccgcc catgtagttg aataccgatg cctggcccat gaactccctt acagcaccgt
    61081 aaaccatcgg tatgcccgcc ttcagggtag cgtcacacag gatataccgg gttgaataat
    61141 tgtcgcagca gtcgactgcg atatcatatc cggcaagtat ccctccggca ttttcttcag
    61201 tgaaccgcac ggagtaagcc agcgcctccg tatgagggtt gatccttctg agcttttccg
    61261 cggcggtttc ggccttgggt ctggtcacgt cgcctgtctc ataaagtatc tgccgctgga
    61321 ggttgccggt actgatctca tcagaatcca caatgcctat cctgcccacc ccggctgctg
    61381 cgagatacat cagcaccggt gatccgaggc cgccggcacc tatcaccacg acggaagcct
    61441 tcctgagcaa ctcctgtcct ttttcgccga tgccggctac cgtcagttgc ctctggtagc
    61501 ggataagttc gtcattggta agtgcggtca tttctgcgag ggtttgtatt tttctcagtc
    61561 tgaataggtt tcggctcttt caagctgttt gcggatcatt tccagaaatg ccggagtgtt
    61621 gcgttcaacg gagagttcgg ggagattgtc aaaatcaaaa aatcctgctt ccgaggtctc
    61681 gatgctgtct cgcagtacgc cgtcaacgag gtcgcagagg actacaatct tataaaagtg
    61741 gtacggcagg ggcggaaatc cgtgcctgtc tgtatcagct attgccagca ccctgttgaa
    61801 gcggacattg aggccggtct cctcgcggac ctccttcaca gccacttctg agggagagta
    61861 atttatgtca gcaaaaccac ctggcattga atacctgccg tcaatttttt ctctcaccat
    61921 cagtaattta ccttctttca gcaccactga ccttatgtcc actttcggag tctggaatcc
    61981 ggtttcgttg gtaaaaagat catgtatttt cgaaggcctg gtgtcacaga cagtaccggc
    62041 aagttcaacg ctgagtccgc gaagttcttc ataccgctcc ctgtcaaatt cactcgcggc
    62101 aaagtgaatt cctgactgtg ctatagcctg cactcttctt gctatcctga gtatctcgtt
    62161 ttccaatttc agaaatgtcg aatgtatgcg ctaacttatg aaaaaatcat gatatcacca
    62221 gtcattgtct gccggttgct gttgaggggc ggttttaacc agaaacggta cggtttttgt
    62281 gcgttttaac ggtaacccag attatgcagt catgaaaaaa gcgatcccgg gcagtctgct
    62341 tcttatcact gccattccgg taatatttgt gtcatgtgac aggctaaggg atgaaccccg
    62401 gccaggagtt ccggataatt tgctttaagc ctggccggtt ttcccacggt cagacagact
    62461 gaatgacaaa atattttttt gcgagtgggg aaacggggga aaagactgct ccggccagtg
    62521 ccggggcagt cttgttattt gtatggtatt atatttgtgc ataaatgttg taccttaacc
    62581 gggaaaataa tgaaaaacac attcttaagc ctggcaatta ttgtggttgc cggcacgacg
    62641 ggacagatga catcatgtga agatcaaacg gaaaaatccc tcccggccga ccccgtggag
    62701 ataaaccttt ccctgaagca gagagaggtg gtagcctctg caaacaggtt tggctttgac
    62761 ctgttcaggc cggtggtggc cggggaggag acaggtacaa acatcatgat ctctcctttc
    62821 agtgtcacca gcgcactttc aatgactctg aacggtgcct caggagaaac attcaacgcg
    62881 gtcagatcgg ctctgcgcta tgacggccag agccttgagg aggtcaatga gacctatcgc
    62941 aggctagtga cagaaatggt tcctgtggat gagcgggtgg taatggaaat tgccaattca
    63001 gtctgggctg agaacaattt ccgggtgaag aaggaattta tggatgccct gaaggagtgg
    63061 tacctggcgg aagcacggaa ttttgatgtg accgatccac gctcagtgga tatcatcaat
    63121 ggctggattg aggagaagac ccacgacagg attcagaata tgctttcatc acttgaccgt
    63181 gacctggtga tgctgcttgt caatgcggtt tacttcaatg gcaagtggaa gcatcagttt
    63241 gacattaata atactgcaga acgtcctttt ttcgttactc ccgggaaccc cgtcaaggtg
    63301 ccggtgatgt accagaagca gaagtttgcc atgtctcgtc aggagaaggt gacactggtt
    63361 gagttgccgt acgggcaggg caactactcg atggtggtgg ctcttcctga tgaggagatc
    63421 gccccttcgg agattgtcac cggacttgat gccgggaaat gggaggaatg gatggaaagc
    63481 ctgtcatacg ggccgactga ggttgagttg tacatgccga aattcaagta cgaatacaag
    63541 cgtaagctga atgatgatct gatcagcctg gggatggatc cggcttttgc cccgggcatt
    63601 gctgacttca gcaggataag cgacgaggag atattcatct cgttcgttct tcaccagaca
    63661 ttcatcgaga acaaggaaga gggcacggaa gctgctgcgg cgactgtaat agggttcacc
    63721 cgtacatcac ttcctcccga gcccgaggta attgacataa acagaccgtt cctttacttt
    63781 atccgggaga cgactaccgg cacgatagta ttcatggggc tggttgctga tcccacaggg
    63841 gagtgaggaa ttgcgatttg agattttaga tttgcggatt gcgaatagtc atgcctgaat
    63901 tacattgact cttttctgag cgagaagtaa cttgccagtt cagccatggc gttgccgtca
    63961 ttctgaatgt cacgtattat gacccctttt tctatgatgg ctatgcgggt gcatatctct
    64021 gttatgtgat tcagatcatg acttgaaatt accatcagcg tgccgttagc ccggttgaag
    64081 tctgacagga ttcgcttcag aactatctgg gatgagggat ccagtccgtt gaacggttca
    64141 tcgaggatca gtacacgtgg ttcagtcacc aatgccgatg caatcccgat cttctggcgg
    64201 tttcccctgg agaaatccct tatgaatttt ttcttctgac caagaacctc accattgaag
    64261 aattcgctga aaccggcaag cctggcttcg gtcagctccg gtgccaaccc gtattcttcc
    64321 gcaataaagc cgaaaaactc ttcgggagtg agatagttta tcaggaatga ttcgtcaagg
    64381 taagaagccg tataatcctt ccacttttca gaccttgcaa caacatgaat ttctgaggtc
    64441 acgcttcctc tgtctgggct gatcaggtcg agcatcagcc tcaacagcgt agtcttccct
    64501 gcgccgttat tgcctaccag tccgaatatt tctccgtcat ggatggtgag ctcactgatg
    64561 ttgagcacgg tttttcgttt gtagctttta accaggttgg ttaccttaat catattctga
    64621 agtttcaggc tgaaagtttt ctgtatcctt ccagcaactt gtacttacgt ttcatgaagg
    64681 ttgcgacgat gatgttttta agccaccatt tgtgaaagat cacaaacagt attcccaggg
    64741 tggctaaaac gattatgcct ccggtttcgc ccgcaaccag cgatgtcagt tcgcagattg
    64801 caatgggtat tatcacaaaa aagaatgtca ttatgaactg gcttcccttt accccctggt
    64861 agttcatgaa agtacttgct tcaaggtcaa cctttccatc gttaagggtg gccagcaaca
    64921 ttgtaaggca actgttgggg cccaggaggt aaatcattaa tgctgtcgtg agaagtacgc
    64981 ttatcctgcc cgaaatatat gacaggacag ctatcgggat gaaggtgata agagtcagta
    65041 ttgcctgcag gtagtatttt gccctgacat aattcaggaa gtcgtttttc cttgccatta
    65101 caccgtcaaa gtacgtgctc tcccagctga acagcaattg cccgtatgac attgccccaa
    65161 gcccgagaaa cattgtggta aacagcatat tgatgtatct ctcctgctcg tcgtaagcaa
    65221 ggaaaaagat gaaggcgtag acaataaaga acagaacgaa gaacatggcg ttcctcgggc
    65281 gtttgttccg gagtatcatt gccatctcaa gcgacatata cctgcctgta tcgccgaaac
    65341 gtgccaggcg tccggtaaat gcatttcggt gcgtccggta tgaagacact gacgatccgc
    65401 ggtcaacacg caggtattga tgcaataacc ggttgataag gataaccgct gcggttataa
    65461 ggacagcggg aataaaataa gcaaggggtc taccttctgt cagtgccatg ccgagtgagc
    65521 gggaaatgtc gccgacggaa ccgcccatcc tgtcaaaaat gaaaaatgct gtggcaattc
    65581 caatgggaat taaccagttg agcggattta tcctggtaag catcccggtc ataagggcca
    65641 gaatgttgtt cattattacc aggagaaaac tgcctccaag aaaaagcaag gctgcctgga
    65701 ttccctcaaa cggtgccgta accttaatca cgaagggcag tataagaaac aacggaagga
    65761 agttaaaaag tgaaaataag ctcctgataa tgatgttgcc tgcaagctta cttctcctga
    65821 tccggaatct caggtagggt acaacatcca gcgatggtac aggctggatg atacacctca
    65881 cgagatagtc cgcaagaaga taccagacta tccactcatg aaatttacct gatgcctctc
    65941 ttccctcacc ggcaatggtt gaactgacat ccaaacccaa cgtcaggaaa atgaagatga
    66001 aatttaatcc caccagggca tacaaaagcc tcgtgatgag gtttcttctc caacgctcat
    66061 tgcggataaa tgacttccag atgtgagagt agagatacgt tccggcttct ttcactcagc
    66121 ttcctttatg catgccaaat tacagatttt tgtatgctgt tgttgtttcc atcagaagta
    66181 tttcggtcca gaaacgaata atttattgca gtgtgggtaa aatgcttttt ctttacattg
    66241 atgaacctga cctatatcga ttttcttgtt agcgccgtgc tggccaccat catgttcggt
    66301 gtggggctgt ctatcaattt ctccgatctt cagaagattt accggtcgcc gaaggcattc
    66361 atcctcgggc ttgtatcaca gatgatactt ctgccgctga tagtattcct gattataggg
    66421 tttactgacc tccatccaac cctgaaggtc ggttttatca tccttgctgc ctgccccggc
    66481 ggcacgactt cgggttttgt caccgtactg ttcaggggta acgtggctct ctcagtatcg
    66541 ctgattgcca taaacagtct cctgaccctg gcaaccatcc cgctgctggt caatcttgcc
    66601 ctgacagtat tcatggggaa acactcgcca tttgaactgc cattggtggc atcattcctg
    66661 cagattttct tcattaccct tctgccggca ataaccggag tactcctgag gtacttccgg
    66721 gagaagatcg ccgacaggat tcagaagccg gtacggaccg tgatgataat cctgtttgca
    66781 ggagtctata ttatcatagc ttttgcaaag gaatcgagcg gtggttcagg gattacaaca
    66841 gaggaactct ggaagatact tccgtatgcc gtgctgatca attttgccgg atttgctgcc
    66901 gggatggcaa tggggctgtc cggtaaatca gggctgagga catcatggac aataggcgtt
    66961 gaggtggccc tgcagaatac caccctggcg ttgctggtgg ccggcacgct gatacagagc
    67021 aatgagatgg tcaagccggc actggtatat gcgctgtttt cattctggac ggcactgctt
    67081 tacggtgtgg ttgtcaagtg gtacgacggt tcaaaactgt tcggggagtt cagaaagtaa
    67141 aatgaattcc gaattgctca atgtgatcag ggtcacaggc aggacagaat tttacccggg
    67201 ccctattcaa caataatttc atcgcaaaaa agccacgcct tgcttcccgc acccgcatgc
    67261 cacggcggac aggtgatctc gcccctggct accacgcgaa cgtacctggc cgtcacgaca
    67321 gggaaggatg aaaaatatgt ctcagtcctg ttgccctgat cggcagggtc aatctctgtg
    67381 atcactgtgc ccaggggagt atagttgctg ccgtcatccg agcatgagta ttcaacgctg
    67441 cggggcagga agatccagct tccgacggcg gccatgaagg atgatgatac ggaggtgatt
    67501 ccagtctcct ttccaagatc aatcaccaca tccatgtcgg ttccttcaaa tccctgccag
    67561 tgcccatcgt tgaagtcgcc gctgccgttt ataccgttga ccagagtgcg atcgccgtga
    67621 cccctgtatc tgtcagagaa gggaatgttg tattgcacct tcattccggt ggccctgttg
    67681 atactgatgt ttttggagct gatacctcct atcatctttc cctctctgaa gatagcagct
    67741 tttactttgg ctttttcggt catcttcaat ggcttgtcat acactggcga gccggcaaca
    67801 ggatctgacc cgtcagtagt atacctgatc tcgggcgatg gttgctctga agtcatctca
    67861 aggataatcc ctgcagttgc ttcgtcattt gaagcagaca tgtcaacgtg gaaggagccc
    67921 cgggaatagt tgatgcctgc cgcgtcaaac cgttccatca ttttcgggag gcgatggttg
    67981 aatccttccc agtcccggct gccagccggg gaccagacaa cttccgaaag tgccgtaagc
    68041 cttggaagaa tcatatactc tgccgttgcg ccatcagata tgtattcagt ccagaggcat
    68101 ccctgagccc cgatgatcat ttttgcttca tcggctgtaa gctcaggagg tacaggctca
    68161 aacgaataga cctttttaag tgtcagtagt ccgcggaacg attccggttc ggtagcgggg
    68221 tctccctggt aaacgttcag atagcagtgt gaaaccgggg ccatgactgc cctgtggcct
    68281 gacctggcgg ctgcaatgcc tccttcgaat ccctgccatg acattactgt ggcttcggga
    68341 gccagcccgt cctgaagtat ctcatcccac ccgataagct tgcggcctct cgaggtcaga
    68401 aatgcttcga ttcgttttac aaaataactc tgcagctcat gttcgtcctt taatccttct
    68461 ttccgtatcc gtgcctggca atcggggcat tttttccact ccgttttatc ggcctcatcg
    68521 ccgccaatat ggatatattc tgacgggaag agatcaatca cctcagtcag cacatcctca
    68581 atgaaactga atgtctcgtc ctttccggcg cagtatatgt ctgtaatggg ccatactcct
    68641 ccggggagca cggtgaaggg ccctccggtg catgagtatt ccgggcaggc agccagcgca
    68701 gacccgacat gtgccggcat ctctatctcg ggcaccacag tgatatgtct ctcagccgca
    68761 tataccacta tctccctgat atcgtcctgg gtgtagaatc cgccgtaggt tgctttttcc
    68821 ccgggttgct gaggctgcct cgagtcccag ggtttgtcct cgcggtcaac gcgccatgcc
    68881 ccgacttcag tgagccccgg gtactttttt atctctatcc gccatccctg gtcatctacc
    68941 aggtgccagt ggaagacatt aagcttatgc atggcgagga tgtcaatgta tttctttacg
    69001 aactccttgt cgaagaaatg cctgctgaca tcgaggtgca ttcctctcca cccgaagcgc
    69061 gggtagtctg ttatgctgac acaggggatg gtccatatca cctcttcttc ctggctgtgt
    69121 gagaaagcct gcggcgggag cagctgtctc agtgtctgga ttcccctgaa aagccccgct
    69181 cccgatccgg cagtgattgt cactttcttc ttttcaacct ttagccggta ctcttcggat
    69241 ctccactcga gggatacatc aattaacagc gagatatcac catcaatccc ttcgttcaca
    69301 gccagttcat aacctgtagc agggcgcagc tccgaggcaa gctgcccggc tgctgccagt
    69361 gcctcctgac tgcctgacca tgaaatgcgg gtaccggatg caagcctgaa ctggccgtgc
    69421 gacaagacca tttcaacagg ttgcggcaca agagaaggag cagtctcctg gtcattgctg
    69481 catgcggcca gcagcatgaa tgctatcaga gcgcagatca ggaccggcgg tttcataata
    69541 ttgtttattt aattacttca atatcaaata tttcatgcct gtcaaactgc agccagtact
    69601 ttatcgaatt ctgcctgacc agttcccagt agatatcgct gtcgagaccg tatataaagt
    69661 taatttcctt cagttcagcg gtgacccttt cagcgagctc atttttaagt gtgatcctga
    69721 cagttctgtc cttatgtgtg aagtcggcag ccggaaagta aggctgtttc ggactgaccg
    69781 tcagcagtcc gtgccccggg gtggctccgg tgctgacctg gatgccatca ttcatgcaac
    69841 tgagcggggg agttgatccg gcatgtgtgg taacctccat ctcatcaact ccggtgcaga
    69901 aatattcgcg cgccctgatg cccatcttta ccccgataat tgcaaacact ccgaggtggc
    69961 gatgcagttc gttggctatc actcccgagg tccactcatc ctcgccatat ttgtttctta
    70021 ttccggaaac gaaaggctgg atgtcaggca tgtaaaacga tgtgtccgcg ggcatccttt
    70081 ttatggtctg cattctttcc acggtctcgc cgcgaagcat tttaagagct gctgtccgga
    70141 gcccgtttat gtccgacggg gtgcggtatg ttatcctctt atcgctgccc tcctggctga
    70201 atagctccgg gaagtgcagg agcagcggca ccatctcatc ataagcggta tatgcgaaac
    70261 tgtgagatgg cattgagttt atgaggtcac ttaccctgaa tgcatatgga gaacggatca
    70321 tgcttatgtt gcccagaagc ccactatcat aaaacccctc tcctccgcct ccggtcaccg
    70381 ttaccggaat ctttcctgcc agaacctttt ccgctgatgc gggatcagac ctgtaattgc
    70441 ttccatcttt tcccggaagg ccgctgttgc tccagtaaat ctgcttcact ttgccggcga
    70501 atgaggggga ttgttccatt gccactgctg cggttttcag gctgcccatc gcaaggaatt
    70561 tgacaggtgt tgtctctgaa gcaagcatct tttcgatgag agctactgca tccccgggtc
    70621 cggccacagg gaggccttca tgataaagcc cgtcggccag gtcccttagc ttttcgtacg
    70681 ccacgggggc cttgtgaaat ccgtccgaag ccgttattgc cagaatcctt acatccggtg
    70741 atgccatcag catgcatatt gccttcaggt catcaatccc gccgtcgctg tcaattatga
    70801 caaaatgcct gggcttccag gggtgtgctc cgagagaaat aataacaaga gaaagaagta
    70861 tggttagtat tgtacgcctc atttcattgc tttttgccaa aaatagcaaa ttagtgcaga
    70921 aaatgctgaa ggctgcggcc tgactgtgat tcaccgattg atatcttccc ttctttccgg
    70981 gcggctttgg cccctggttc ccccgccgca atctcttcct tatcccacct gcagttgaag
    71041 aaaatctctt ttttactatc ttgacaacct taatttccgt catgtgaaga ggaagacccg
    71101 gatactgttt tttatcggcc ttgtggccgg gatggttatc cttgccggat tcggcaaggc
    71161 agttgaatat acttccacca atgatttctg tgcggtttgc cacgtccatc cgcaggcaac
    71221 gacctcctgg aagcagggta tccactacgc cactcagtcg ggctaccgta ccggctgcgc
    71281 cgaatgtcat cttccgccga agggtgaggg ttacctggtt gagaaggcga agacaggaat
    71341 gcgtgacctg tggagccact ggacaaagga ttctgcagat tttgactggg aggcggcatc
    71401 gacacttgag agagcctcga aacacgttta taattccagt tgcatccatt gccatgaaaa
    71461 tctcttcccg gcaaagctga cacgcgatgg atccgaggct cacctctact acaagcagat
    71521 gactgaaaag ggagaggatt tgcagtgcat aagctgccac ctcaatgccg gacattatat
    71581 cgagggttat atccatggga gcaacaccgg gtttggtgct tctgtggcca gggcggacac
    71641 actctatcag tacccggcca aggtgacggg ctttaaagat tttaccgagc aggtgccggg
    71701 ttcggcagtt accttcagga tgattgcggt gcctggaggt gagttcacca tgggcagtcc
    71761 tgagggtgag ccgctgcgcg gcgaagatga ggctgagagg atggtaaagg tcgattccat
    71821 tttcattgcc gaggtggagg tgacctggga tgagtacatg gagttttaca ggcagacggc
    71881 atcggaaggg aggagtaccg atacggaagg tgcgagggca aaagctgccg gtgttgacgc
    71941 catcagcgga gccacccctc cctatggcaa acccgaccag aactggggta tgggacagcg
    72001 gccggccatc tcgatgagct ggcatgcggc ggagacctat tgcaggtggc tctctaaggt
    72061 taccggcaaa aactacaggc tcccgaccga ggctgagtgg gagtacgtct gccgtgcagg
    72121 gactacaacg ccatacttct tcccgggaga ccctgctaag tttgaaaaaa agggcctttt
    72181 cggaggcaaa cctgatacgt cggtgataag caccttcgtg atctaccacg gcaacagtcc
    72241 ttcaaaaacc caggagccgt cggctgtgaa gcccaacccg tgggggataa ggaacatgag
    72301 cggcaacgtt gctgagttct gttccgatat ctatgaggga accagtgagc atgtcatcag
    72361 gggcggatca ttccgtgacg gggctgctgg tgtccgcagc gctgcccgtg accatacccg
    72421 caccgatgag tggctgaaga ccgacccgca gatgcccaag agcatatggt ggtactctga
    72481 ttgttttcat gttggattcc gggtcgtaat ggattggaaa taaggcagta ggcaataggt
    72541 ggcaggcaat agtgacaacc gagaacatcg caaatcgcag gtcgcaaatc gcaactccag
    72601 atcgcacatc gcacatcgca gatcgcaact ccaaagacct tcagacccca gaccttcaga
    72661 ccccagacct tcagacctca gaccttcaga cctcagaccc cagaccttca gacctcagac
    72721 cttcagacct cagaccttca gacctcagac cttca
//
LOCUS       DAOPXU010000003        59543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_379_length_59543_cov_11.804650, whole genome shotgun sequence.
ACCESSION   DAOPXU010000003 DAOPXU010000000
VERSION     DAOPXU010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 59543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59543
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_379_length_59543_cov_11.804650"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(496..888)
                     /locus_tag="PKK03_00610"
     CDS             complement(496..888)
                     /locus_tag="PKK03_00610"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNX82918.1"
                     /translation="MKASKEEYIRYRIEKAWKTFEDAKLLAKTGSWNSSMNRLYYACF
                     YSVLALFAKHNLNTQTHSGVKTQLSLHFVKPGLLDKELGMIYGDLFDLRQRGDYGDFY
                     DFEEKDIISIIPKVEEFLNTIDDLISMD"
     gene            complement(891..1199)
                     /locus_tag="PKK03_00615"
     CDS             complement(891..1199)
                     /locus_tag="PKK03_00615"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNX82919.1"
                     /translation="MNTTITSRITTLVREIDPSAEVILYGSRARGDYHPESDWDILIL
                     VDSEVDLNYERLFRHKLYDLELETGEVFSTTVYNRADWRTRYWMTPLFKNVSKEGIRI
                     "
     gene            complement(1517..2476)
                     /locus_tag="PKK03_00620"
     CDS             complement(1517..2476)
                     /locus_tag="PKK03_00620"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HNX82920.1"
                     /translation="MNRTFWLIISSLCVFSCKNQEMPVSYLRSDMRIDRLSDSSYLSD
                     VRSMVFDRYLYVTDYERNQIIKLTPQGELAGYLGAKGKGPGEFLGASDIIVQGDSLWV
                     YNDGNGSFEVFRSDGHLQTIRLPDNIDYSGGFNFFLFRNRLFMSSVSETNSLCSFDVK
                     TSAIELFGQPKVFDTDIHTRIRNHRHLSGTDSCIFAVSDNMPVIEKYSHSGQLLETLD
                     YSSIPIVRERLAVISENTTPNGYTLLVEDVCYNDGKLYLLLYANRGNRISCSDILVID
                     VSDRNMSCQRLLNLGDSWYETICVSGIRLYAFSNNGLERFIID"
     gene            complement(2563..3798)
                     /locus_tag="PKK03_00625"
     CDS             complement(2563..3798)
                     /locus_tag="PKK03_00625"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HNX82921.1"
                     /translation="MRILCILIACGTFMLLSCKNKDSSSSQVDLVKLIKLDLSKARSD
                     SMNLSEIATRIEYIPLERNDQALIGHVYDFEITDDCIFVDFEKDLFRYSSDGKFLNRI
                     YTKGRGPAEVQPVCYAISKEDSLVYVFSWDRSLKVFDFNGNYVTSITRPINPEESLPP
                     WRIGIFNNTIFVAVAQEPWVKYIYSCYDMQKDTLHVLYKNIREYKDDQSERNPAIIPY
                     DFHYQINETTLLFKEKFSDTIFTANREFEVNPRYIIYIANDKLAWEVWRDNAMFDISG
                     GPPAGYWMQSFSETDRFLFLMLVSHKGPQVLVTNDRNTGATEMNTLKEFKRAYTRVYL
                     KNDIDNLIPFAPVNENGYFISSGNCMYSIVDGDEFSRYYRNASEATRNDTKYLRQMAP
                     ILDTIDEFSNPVIMKVSLK"
     gene            complement(4120..5277)
                     /locus_tag="PKK03_00630"
     CDS             complement(4120..5277)
                     /locus_tag="PKK03_00630"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HNX82922.1"
                     /translation="MKASLFVLTIFLVFSCGSPDNEIIMIDLENLNGEEITLSEIADE
                     ITYIPLDNSITIGMINSFYNPIVIGNLVYLYDHETGIMLFNRIGELHGIIGTMGRGPG
                     EYVRGNKFAVDENTGRVYVCDAGNIIKIYSGSGSFQRSISLQSYGGSVDAIGLIDSKL
                     FVTYNMQYGDAEFEWVLVDSTGNLLMNKRRTDDFFSSNYLAGGGTYIVGSKLNYWSNF
                     IDTVFSISDDLTINPEFIIKPGDYRLPRSRVEDPLSQLSDYMTIDQIFETRSFITIRY
                     SFYRGKNGCVLVDRYNHDTYLSYWERNGSGGIENDLDGGTKFLPKGYFEENGREYLVG
                     LVEPVTLKTRIAGSEFRDIIPKCPEKKEELERLADNLKDTDNPILVLVRLN"
     gene            complement(5384..6016)
                     /gene="pnuC"
                     /locus_tag="PKK03_00635"
     CDS             complement(5384..6016)
                     /gene="pnuC"
                     /locus_tag="PKK03_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNX82923.1"
                     /translation="MFNIDTTLFELLGYRMSLLELLAVLTGLAAVWYAARAKIITWVF
                     ALVNAVLFFMLYYQVNLYSAMVLQIFFFCNAIYGWFNWRSKSSNGDEPVTLLSHKARV
                     FWVAGIIAMALAMGTVMGKIHLWLPDFFPVRATFIYTDAMIAVMSIAASLFCARLKLE
                     NWILWILVNIMSIAMYAMRGIMLVSVQYIIFLAMAIYGFVQWERKVKLQW"
     gene            6229..8013
                     /locus_tag="PKK03_00640"
     CDS             6229..8013
                     /locus_tag="PKK03_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82924.1"
                     /translation="MSPREVYQSREAEFSTAAEQLRKLSDRLSMARLTAFAGGLVVFA
                     VLLSFSVITAFAILTAALILFAWLVIKYETTEKSRKRFLQLAEINRLELKCLDGDFSG
                     FKTGEEYAERDHPYTYDLDIFGKASLFQFLCRATSRPASDRLAEHLKQPAGPEEILLR
                     QKAIAELQPLTDWRQELMTLGYMNAGAGNDPGPLMQWIGSDDLFRKTGREKVFTGCLS
                     LLAVTAITSVIAGLPAAILAPVFVVNFIFYFTRFRRISRLQEQVSRSSDLLKGYSVII
                     KLIETRNFTSPLLQSLKSTFMEEIAASDRIRQLSKLVGRLDYRLNVLVSAPLNLLFFA
                     DIHFCLALEKWKREHALRIPSWFSSMAEFEALASLANMAFNNPGWVYPQVTEDYFVFR
                     AEDMGHPLIPANRRINNSFATEGAEKAIIVTGSNMSGKSTFLRTCGVNAVLAFAGAPV
                     CASAFTISLVRLHSSMRISDSLEENISSFYAELRRLRAIISGAESDPKVFLLLDEILR
                     GTNSDDRYTGSVALIKQLTGYGAVAMVATHDLRLAGMEQELPQAIENYHFDVKVNDEE
                     LFFDYLLTPGICSSFNASLLMKKMGIKV"
     gene            8246..9463
                     /locus_tag="PKK03_00645"
     CDS             8246..9463
                     /locus_tag="PKK03_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX82925.1"
                     /translation="MIRQDEIAVVIDAQKETLLRQEPGLRRDALTGIPVAESFATIIT
                     GMRRCGKSTIIMQLLHKNHQDTIYLNFDDIRLSGFEAGDFSRLHREIETRTIKTLFFD
                     EIQIVNGWEKYINQLLREGYKVFITGSNASMLSVELGTFLTGRHLSMELFPFSYNEYL
                     RFKGLDSGESSVLDYLKTGGIPEYVKSEIPLILNNLVDDILMRDIAVRHSVRDLASLR
                     QLTAFLINNIGNLISANKLAGLFNTKSPATLLEYFSYLKDAYLVDFIPVFSHSLKVQA
                     RNPKKVYVMDTGLYTENSLSTSENLGRRLENLVFLHLRRRYQHVFYYRNNGECDFITL
                     EKNVVKEAVQVCLAVNEDNFKREYNGLTEAMHNLGINNGCIVTLNQNDIFVENDLKIR
                     MIPVDEYLSGGQD"
     gene            complement(9652..10578)
                     /gene="miaA"
                     /locus_tag="PKK03_00650"
     CDS             complement(9652..10578)
                     /gene="miaA"
                     /locus_tag="PKK03_00650"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008020907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNX82926.1"
                     /translation="MDNPMHDILVITGPTATGKTRLAAMVADRMGGEVISADSRQVYR
                     GMDIGTGKDLSDYTVDGRRVPVHMVDIVDAGYRYNVYEYQRDFLRVFEDMSVRCVFPV
                     VCGGSGMYVDSIVSGYRLVQVPVNAQLRALLAGRSLEELTEILSQYKSLHNKTDVDTV
                     KRAIRAIEIADYYSHHPVDERPFPVRNPLVIAVFVDRETRRKHISERLRSRLEEGMIE
                     EVQRLLVGGIHPDDLIYYGLEYKYITLYLMNRLSREEMVQKLEVEIHRFAKRQMTWFR
                     GMERRGIAINWIDGSMSDPEKTAYIVAKLYHT"
     gene            10658..11239
                     /locus_tag="PKK03_00655"
     CDS             10658..11239
                     /locus_tag="PKK03_00655"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNX82927.1"
                     /translation="MDLRFLYHRTKYFIINPGKAWEVVHREERPMRFVRGSFFMPLII
                     LVSVSAFLGSMFFINTTLKPMYSVLAAANTFLFLYLGVYGSAFVVKEIMKAMDLSHDF
                     LVAFKLVAYSMAPIFLSLTVSRLFESLLFINVLGLYGLYIFWVGMEVMVNPPDHKKLP
                     MIIASVVSMLIIFFLLQVILSKLTQTIYFSIFA"
     gene            11248..11715
                     /gene="smpB"
                     /locus_tag="PKK03_00660"
     CDS             11248..11715
                     /gene="smpB"
                     /locus_tag="PKK03_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNX82928.1"
                     /translation="MRNDNSNIVIKNRRASHDYEFLEKLIAGIKLTGTEIKSIRAGKA
                     NLTDSFCAFRNGELFVTGMHISEYYWGNLNNHDPLGERKLLLTRKELRKWERKVNETG
                     LTIIVTKVFINQRGLAKAEIALAKGKKQYDKRDTLKKRDHDREMDHHVKERGR"
     gene            complement(11716..12432)
                     /locus_tag="PKK03_00665"
     CDS             complement(11716..12432)
                     /locus_tag="PKK03_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNX82929.1"
                     /translation="MDEAKEKDLIKDAFEPRTWNEIHTMDSWRVFKIIAELVEGFEKL
                     SRIGPSVAVFGSARTHFNNKYYKLAEEIAFQLTQKGYGVITGGGPGIMEAANKGAKKG
                     NGKSIGINIDLPFEQKANPYIDRDKLITYDYFFVRKVMFMKYAQGFIVLPGGFGTFDE
                     LFEAITLIQTKKIGRFPIVLVGTKYWSGLFDWVTEVMLNEEHNISPEDLNIFRIVDEA
                     EEAVGYIDQFYSRYLLSPNF"
     gene            complement(12466..15300)
                     /gene="uvrA"
                     /locus_tag="PKK03_00670"
     CDS             complement(12466..15300)
                     /gene="uvrA"
                     /locus_tag="PKK03_00670"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNX82930.1"
                     /translation="MPEQKDEIRVYGARVHNLRNISVTLPRNKLVVVTGLSGSGKSSL
                     TFDTIYAEGQRRYMETLSAYARQFLGGMERPDVDRITGLSPVISIEQKTTNKNPRSTV
                     GTITEIYDFLRLLYARAADAYSYESGEKMVKYTDDQILDLVAERYDQHKVYILAPLIK
                     GRKGHYKELFDQLRRKGFLYARINGEIREITQGLKLDRYKVHFIELVIDKFQVGHVTE
                     KRLHDAVLLALDQGDGIMALLDAGDNSLRFYSRHLMCPVTGISYNEPAPHTFSFNSPQ
                     GACPKCNGLGEISEVDISRMMPDPLLSIRKGGIEPLGKYRNIWIFWMIEALGEKYGFT
                     VDTPVGEIPEDVQNKIFFGSDEVLKLSNTPLGVTSNYFMTFEGVVNYVGNMEAMSGRT
                     ANGTGKSREQYVQYHVCPECNGMRLKKEALWFRIGGKNIGELAAMDLTELRDYLENVT
                     GDMSERQKKIAAEILKEIRSRLGFLLDVGLDYLALNRGARTLSGGESQRIRLATQIGS
                     KLVNVLYILDEPSIGLHQRDNRRLIEALRSLRDAGNSVIVVEHDRDMILSADHIIDIG
                     PGAGVHGGMIVAEGTPEDILKADTLTAAYLNGAKTIEVPAKRRDGNGKFLTITGATGN
                     NLKRVTMKVPLGTFICITGVSGSGKSSLINQTLHPALAKKLHNAGRMPLPYESIEGLE
                     NIDKVIEVDQAPIGRTPRSNPATYTGVFTEIRNLFAMTPDAKVRGFGPGRFSFNIKGG
                     RCEGCEGAGVKTIEMNFLPDVYVMCPDCGGKRYNRETLEVKFKGRSINDVLEMTINDS
                     VEFFKGIPSIYHKIKTLQDVGLGYVRLGQPSTTLSGGESQRVKLATELSRRDTGRTLY
                     ILDEPTTGLHFDDVKVLLEVLGRLVERGNTVMVIEHNMDVIKVADYIVDLGREGGHGG
                     GEIIFSGTPEEIIKQKDNYTGQFLAPVLEH"
     gene            complement(15310..16248)
                     /locus_tag="PKK03_00675"
     CDS             complement(15310..16248)
                     /locus_tag="PKK03_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNX82931.1"
                     /translation="MKITAGKAAIAIMFLLSAVFLFTLLVSADSQQQEATDYEESAPL
                     AVSWAIPENVTFAGEALPLQNFDTRESLDRELNATAYRHGSTLLTIKRSGRYFAEIEK
                     ILKEYGIPDDFKYLACAESDLSNVISPAGATGYWQIMESTGKESGMIINREIDERYDL
                     DKSTRFACKYFLKAYERYGSWTMAAASYNNGFSGLSEQVDIQKETNYYDLLLNEETAR
                     YIFRIVSLKLVMAEPAKYGFNIGPGELYKPIPFREVKIDTAVTSFEQFARQFDTNYKL
                     LKFLNPWLRRPFLTNSEKREYTIRVPEKGARIQAGL"
     gene            complement(16333..17643)
                     /locus_tag="PKK03_00680"
     CDS             complement(16333..17643)
                     /locus_tag="PKK03_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82932.1"
                     /translation="MFEREPNIDIVFRNGLKNLEVLPPADVWDNIPPMPVKHSRLRII
                     SGIAAGVAALVSLSLLATWYVRNNSTPLLLSEVTAPGRDIRDVSLDIAKAAPVTRPAE
                     ARIVNLPATTAPSEPAGLSDMPAMEEAPTELIAYADLPERDVLPDEPVQLFSDEITII
                     NAGRFKGAGENLKTTLPDLKGAKADQRFLLGASLSPAMGFSRSGDDARLAELINSEKS
                     RPAYTTGVSVAYRISDRLSIQSGIGLSSIGQTISDVAVYAGLSDYYSVKSSYLYSVET
                     AAGLILAGNTDLYLADSKARVGTLIPGNMADPSKYRLTQVGSDIQQVFRYLELPVMIR
                     YKLIDRKVGLNVSGGVAYGFLVDNSAYTGEGSDLIRIGHTEGVSNFNLSSQLGLGMEY
                     NISHKLIFNLEPVFRYYVTPLSEISGSLYKPYSVGFSSGFFFKF"
     gene            complement(17636..18163)
                     /locus_tag="PKK03_00685"
     CDS             complement(17636..18163)
                     /locus_tag="PKK03_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX82933.1"
                     /translation="MTGSRRDQELLYRRYSPKLYAVCLQYAGNTEEARDVLQEGFIKI
                     FENLSHFSHEGSFEGWMRRIIVNTALERYRNRYYLNRVDDIDEVTEPEAEPDTDDFAG
                     LEAFDLLNMIMELPPKYRMVFNLYAIEGYSHKEVGEMLGISEGTSKSNLSRAREILQK
                     KVMILTGVTKRMVHV"
     gene            complement(18473..19867)
                     /gene="lpdA"
                     /locus_tag="PKK03_00690"
     CDS             complement(18473..19867)
                     /gene="lpdA"
                     /locus_tag="PKK03_00690"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNX82934.1"
                     /translation="MNEFDLIIIGSGPGGYVAAVRASQLGMKVAVVEKAETGGICLNW
                     GCIPTKALLKSAQVLEYTRHAADYGINAGEAPAPDFGAVIARSRGVADAMSKGVQYLF
                     KKYNVPVISGFGRLKGAGAVEVEAADGTRVSYSAKHIIIATGARSRELPNLRQDGMKI
                     IGYREAMNLPSLPASMVVVGSGAIGSEFAGFYRAMGTEVTLVEFMPRVLPIEDEEVSR
                     TLERAFKKAKMKIMVNSTVEKVDTSGEKCIVTIKTPKGEETVETEIVLSAVGITPNIE
                     GIGLEEAGVKTDRGRVVVDEFYRTSVSGVYAIGDIVPGPALAHVASAEAIVCVEKIAG
                     RDPHPVDYANIPGCTYTTPEVASCGMTEEAAKAAGIETKSGKYMFMASGKASAAGARE
                     GFVKLVFNAADDKLIGGHLIGMNVTEMISSLVTARKMGATAHDLMTSIHPHPTMSEGI
                     MEAAAAALGEAIHG"
     gene            complement(19864..20703)
                     /locus_tag="PKK03_00695"
     CDS             complement(19864..20703)
                     /locus_tag="PKK03_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNX82935.1"
                     /translation="MENLFEYSFVLRSLLGALFASITAGLAGTYIVSRRLVFLSGGVT
                     HASFGGIGLGYYLGINPVVGAAVFGLGSAIGMEYMSARGKMREDSAIGILWALGMAVG
                     IIFIYLTPGYAPNLMSYLFGSILTVTDGDLIALGIISVVLIVYTAVFYRTLLYISFDE
                     NYARTFTRHVDLFRYVSVGLTGLAIVLNIRMAGVIMIIALLTIPANIVMLFTRRYLRI
                     IVLSTVVSFFGISAGFVISWFTNLPTGATIVAVLVLFWLVARGARSLIRSSNIEKLKK
                     TTI"
     gene            complement(20703..21449)
                     /locus_tag="PKK03_00700"
     CDS             complement(20703..21449)
                     /locus_tag="PKK03_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HNX82936.1"
                     /translation="MSILFTIDSVTAGYEEKVVLHDVSMAVEENDFIGIIGPNGGGKT
                     TLLKVILGLLKPLSGRIDYSPGDLRLNQIGYMPQVAQGDTLFPVTVEDVVLSGMMHDR
                     GAMGRMGRTEKKRAAEVMDELGLRPLAHQALNELSGGQLQRVYLARAVSGSPRLLLLD
                     EPATFVDASFEADFYEKLKLLNNRMTIMMVSHDVGTISAWVKSFACVSGKVFYHPHSK
                     ITDEDLRMYGCPIQLVTHGDVPHVVLHKHD"
     gene            complement(21541..22524)
                     /locus_tag="PKK03_00705"
     CDS             complement(21541..22524)
                     /locus_tag="PKK03_00705"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNX82937.1"
                     /translation="MKKISLWLVFIAIAAVSCGPRSPRGEEGRVVTVTIPPFAWFVEQ
                     IGGDDFKVNVLLPPGADHHIWEPLPAQISSLSGSEAFIMNGQLGFEHAWMDRFRQVNP
                     DMKVLDLSRHIKLLGPRHPSGIGVSAPPGAEPQSEGLRSEGLRSEGAGHEEGDHEDHG
                     HAHGGPDPHYWMSPSAAFIMARDVHDFLVGLNPASSEKYDSNLYAINREISEVDSLVR
                     VALGPGPHPTVMIYHPALAYMGRDYGFEQVSFEDEGKSPSPARMKQLIDLARENEIKI
                     IFIQAEYDVRNAESLSRETGARLVVINPMNSDWPEAVMEIARAVGGKDFRF"
     gene            complement(22764..23537)
                     /locus_tag="PKK03_00710"
     CDS             complement(22764..23537)
                     /locus_tag="PKK03_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82938.1"
                     /translation="MKRTVNFISVAVMAIMLSSAGAFGQETSTKTADEKKKQEELQKQ
                     AELQKQAEYQKQQALTEELKRKQADIEAKRKEIQEKMKEIDAKSVEKNKELEFYYQQY
                     ADMSRHAGNLYPDMPVVVMPELNDLNEYIDLGRDAGSNIFLYKGQGLGSRPGSSWNYS
                     RQVMEASFTNEMTMSADDAKSVNLSVSGDCAEGSMAVTIIMPDGKQLSEVVLDENGSL
                     NWRKNFEAGEGNGWKNGKWTFRIKAKEATGNLRISMNSY"
     gene            23672..25033
                     /locus_tag="PKK03_00715"
     CDS             23672..25033
                     /locus_tag="PKK03_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNX82939.1"
                     /translation="MKRQLLFAVSLAALLLFVAVQLYMIRNTWKQKEEIIMMRYSSLS
                     REGLSLLLAKKKSNGFEKAMDITDKFADYLISEELCKLYTSYDSASLSRLALKETYKI
                     MEENEQLTSFFRSYFGNAGYDNDFIPVIAISKLVLLVPSDKMVMADTVYSKPGKTMVL
                     VNTFREEHNNFIIEYHYLLDLTHKNEMVLREALLSFLLIIGSILIVFLVFGLTWRNLL
                     EERRLSELKSDFINNMTHELKTPLSTITVAGRTLEKEQIISDREKVLETAQLIGKQSV
                     HLNQLINTILEVSFLERQEFELDRHKVAIDELLHQVVSSFLTSCDGCAVIEEDYTTNG
                     LEASVDIVYFTTMISNLLSNAVKYCDREPLIRVTAAVSGCDVEITVLDNGVGIPQEHL
                     GHVFEKFYRVPQGNIHKAKGLGLGLYYVRRIAMAHGGDVSVTSKPGRGTTFKIRIPIQ
                     NTI"
     gene            25030..25704
                     /locus_tag="PKK03_00720"
     CDS             25030..25704
                     /locus_tag="PKK03_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX82940.1"
                     /translation="MKLLLAEDDIDFGNILSQYLSMSGFEVTLARNGREAWDRFHDSK
                     PDICVLDIMMPEMDGFTLAEKLRAKDPGIPFIFLTAKSLREDMIRGLKLGADDYITKP
                     FDPEMLLLRINNILRRIYTVAEDEYRISDTTLRYNALELITPAGKEKLTLREAQLMRH
                     LMMNRNKALTREQILTEIWGEDDYFLGRSMDVFISRLRKYIAPDHGLSLRTLRGMGFI
                     LEELRQ"
     gene            complement(25791..28613)
                     /locus_tag="PKK03_00725"
     CDS             complement(25791..28613)
                     /locus_tag="PKK03_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82941.1"
                     /translation="MSEIPVGGTLRLLIQVICITFLSVPVQVQAQAVGGRNDISRFIP
                     GTIPVKSQNWEISRDPVSGYVYFANSAGLIEYNGITARSYPVPDRQGARSVYVNGDGV
                     IFTGSFEDFGFWMKDQSGVLSYTSLAGSADVAENDEIWNIFEIGRTLYFQSFTSVYVY
                     DYNGVKRIPAPSSMLFFFRSGERFIAQSIDEGLYWFDGTGFRFIPGSEIFATHKIHAL
                     IDLGQDEKWICTANDGIFLFDGAKFTPVANEISGYLKEATCNAGLAVNDSLLAFGTIL
                     RGLVLCDRSGRILETYDYSNGLSNNTVLSLYQDAGKGLWVGLDDGANFLDTNSPVAFY
                     ENSGGDLGTIYTVIRDRDYLFLGTNHGLFATDIENVNGSYRFSGLRIIPGTQGQVWKL
                     LQYDGQILCGHNDGTFLIDGLNAVRISDVTGGWSVRPFGEYLVQGTYTGLVSFSRGAR
                     GKWEYCAKIEGYAEPTRFVEIDYLGYVWALHPQKGLYRLELNEEADSVTSSLHFSQAG
                     DSARIISMAMMNNQMVFIAADHLYGFDYERKDFFPVTSLEPGLGEFVKATQIIPFQKN
                     SYWFVLDNRIALFSITRDMQAEKVMEFVHEYADLPWREQQVMSLDSGMLLIPTRQAFS
                     IYDVDRLVNSSEASALAVSRLVFSGGNRSATLFPQSGDERQVPSRANNLTVYIANPSG
                     FDREVREYLYRITEMGEEWYRTATDNFSLLNLKHGEYHLQVREAAGRGMTETRFTIRR
                     PFALTGWALVLYLLAASAITAAGIMFFRSKLEGHRRMIEYEVGKNRLESELDYKSYEL
                     MLTMRYLIRKTDTLRELRDKLETAKESSVKMPARFIREMEQIIDHGLDTQTEEWQNVM
                     KNLKLSQEGFFRKLKARYPALTPNDLRLCSYLRMNFTTKEIANLSNISTRAVEIARYR
                     LRSKLNLSHDVNLTEFLIHEAEISED"
     gene            complement(28675..32079)
                     /locus_tag="PKK03_00730"
     CDS             complement(28675..32079)
                     /locus_tag="PKK03_00730"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNX82942.1"
                     /translation="MKISNNAGLTVEFLGNGAVSCIHTDTIMISLRQATVYTGAYAGI
                     FLRKRDGAIESHLLTGPGSEGYYRVENSIYSVAGSWDDIGYICSLRLSEKSLSWEWTT
                     ELFNNSGREVTLDLVCLQDAGLKAVSQGLVNEYYVSQYTERRILDDPEAGKVVCCRQN
                     MKESGGWPWLMMASLGRAGSACTDGMQFYGKSYRATGIPEGLLAESLGGEYAGESSVI
                     AIQEEPFVLRAGETRRVRFALTFLHNHPPATSEADLDRLPGLAMEFSHDHSGAAGSLH
                     SEALFSSFREPGGLSGIEGFRKPELNLFSTPVILQAEELTEKEIENFFPGPRRYSEFS
                     GGRLLSFFYDMNRHVMLRGKELLADRPHGHIMHNRAHLLPDESIMSTTAFASGVFNSH
                     ITQGNTNFNTLLSVCNSQFNINPVSGQRIFVVAGGKRMLLGVPSAFEMGLNHCRWLYR
                     HGNSLYQVQTWTSGKVPQVNMDFRVLEGDPVALVVTHDFDELNGWSIRPDAVAGEYIA
                     LPAAGSMISRKFPDARYRITIQYSGSRYRAGGNGFSGKDDVLNDNSLFTVETAPLTRF
                     SMSFTGCVTAGIEPVFFDDADRQFASDAGDASGEWREMSRSLSIRSSDDDISAITEIL
                     PWFGMNAITHFLTPYGLEQFSGAAWGTRDVSQGPVELLIAMGKYEEARNVLTVIFSNQ
                     EPDGGWPQWWMFDSYDEIRADSAHGDVIYWCLIALSSYIRATGDFGFLDKSLPYFQKD
                     NEAQNLINTLREHINRLVKKVTGSFVPGKSLVRYGGGDWNDSLQPVNRELADRLISSW
                     TVEMNYQAFRQYAEVCALAGDHDGAEEMNSCSENIRTDFNRYLLRDGVVAGYGLAGDD
                     GRISLLLHPSDTVTGISYSLLPMERGIVSGIFTPEQAASHLELICRHLTGPDGARLMD
                     RPLRYSGGPEKIFRRAESSTFFGREIGLMYVHEHIRYAESLARTGRAVEFMKALRQAI
                     PVGYRDIVSNSDIRQSNCYYSSSDAIFETRYQADELYNEIIEGKITLRGGWRVYSSGP
                     GIFAGIIMTHLLGIRREWGRLIIDPVMPASLDGLEASMIIMGRTVTFVYHVIEGCCSP
                     KRITVNGSDVVFTPVENQYRPGGAAVDERVFEGMPSGSEDRVEIYL"
     gene            complement(32167..34599)
                     /locus_tag="PKK03_00735"
     CDS             complement(32167..34599)
                     /locus_tag="PKK03_00735"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNX82943.1"
                     /translation="MTFSVKDNNFYLGGRRIFLNSGEIHYFRIRRGLWDKHLDEAVSA
                     GLKAVSTYVPWAWHEEEEGVFDVDGSSCPEKDLTGWLRKCKEHGLHAIVKPGPFILAE
                     FRGAGLPDWFLERYGDGVRMHTRRGVRVMSDGVSLFNKDYLEKVRLWYDQVMPVIRNH
                     EIQAGGSVIMMQVCNEIGVFSWLARQADYGNEVRNRFISWVSGKYGTVSEVNRQWGTS
                     YSSFDEIELPPDGREPYASPADRGRDNAWHSFWRRYYGDYLRMLSSMIRERGVTVPLY
                     HNLPGWIYGNGYEFPVNITMYEDLFRGRSEIIFGVDHIPEYVSYRNLHDDRIINDITL
                     AMQGGGPLFAAEFQSGSREYHVVTNPREMELFYKASIANGLKGWNFYMFSQGRNVSRR
                     GYSGDTFYWFTPLDAEGKRGSAWPAVQKMSHMTEVAGDIIVNSGRRARVCVLFYPHWY
                     ATELERPAGEASGLTYIPSAVRRPAYFDGLLKALQVLNVDYDMADLSTASPESLSQYR
                     QVWVFATDEMDAPAQMTIAGYITGGGHAVIYPYLPDRDTSQQPCKVLRDAIGADPSGT
                     EVTDSPLIDLFDLHDIKCNNPQVVFTSESLSGAEVIARTLRGTPCGFEKRVKKGKVTF
                     IGTWAGFDTEAHRAFYEKLLERSGARLRNAASDNGFIIARERFTGDGRALLFVANYYN
                     EEMSGSVTYTHPASGETVRLPLSGEKISWPPLRAILTPVCLQVAEGVTLLHTTSDLLG
                     LRREGNEIIMTLCGDRDLAGEAVLEGPGALRIKRTEIGGKPAETGVTGGHMVITYSHR
                     HGEELQLRITLG"
     gene            complement(34614..36704)
                     /locus_tag="PKK03_00740"
     CDS             complement(34614..36704)
                     /locus_tag="PKK03_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX82944.1"
                     /translation="MEDTKTSRGKAVQGYDRITFGQLAAYSAGGIIPVALFNIAGQLV
                     GLMGNISLGLSAFWLGLIMIIPRLWDAFSDPIVGHLSDNTRSRFGRRRPFLLIGGIAV
                     AVFFVVMWWIPKGETVHAWFPSETGFQSFQLFYILFALLLFFTATTVFEIPHGALGME
                     MTSDPHERTRLFSGKSFVGNLFAMSTPWLFALANMEVFRGPGGNEADGMRYVSLMIAA
                     VLIPLSFWWTAKLREPGFVRVAKQEKTHFWQDMKVVGTNKNYIYLVLTIFTLAMGFNF
                     VNLLGSYIPIFYVFGGDKVAGARLLGINGTIWAITGVLAVFPLNWISPKLGKRNTLII
                     SMLLMTLAQLSKIVCYNPEHPYLVIIPTIMLSAGMLFFFTLGSSMVGDICDEDELRTG
                     HRTEGSYYSIYWWFIKLGTAFASFVAGVLITLTLFDETQVTKVDGLQGSVRDMQAKVS
                     YWKGYTGDAAAAGTWLKNAQSQAADALKESKDYLVYLESEAVKKPGRKKVITPEIEAQ
                     RKNVLMNANAVTRQNIAALERNIEALASATAGVPAPAADSLIRSTVPLIMQSKLTKAR
                     ISSFDLLVHLRTRSNEQKKSRDHTEHLIGSVAEMNRMIYALEPGSPLDVIETSLAPIS
                     AGVGPLKKQSPYTLLMMRVVEIGLPVLLCLLALVFAFRYSLTEKRTMEIKELLREKRG
                     IGNNGGQAEGLQST"
     gene            complement(36726..39989)
                     /locus_tag="PKK03_00745"
     CDS             complement(36726..39989)
                     /locus_tag="PKK03_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNX82945.1"
                     /translation="MNYRTRKTEALLLLIALTSFAATAQQRTLETFDQASGWSYNISD
                     GVTLNVSAEKGVTGNAVRIDYDFTGGTGFGGIQKMFPINLPENYEFTFWLRAESPSNN
                     FEIKFIDSTGNNVWWVNNRNYDFPQEWTKIRIKKRHISFAWGPTEDHDMKRVDRIEFT
                     VASFVGGKGTLWLDDLKFEPLPPETDVWPEPVMSASSSARGHRPGLAADGSGESFWQS
                     RKAGKQSITLDFKGRREFGGLYIAWFEGSHAEEFDVLLSGDGKRWEKAYSVSPNRGGS
                     SIIRLPEAEAAYLRIDLLKPANDRSYGIREIKIPDVRSTLTSNDFIMYAARNSPAGSY
                     PDYFSGRASYWTVTGVSSDTKEALISEDGMVEAEKARFSLEPMLKTGNTLINHSNVQP
                     EQVMGFPGYSGDYVFLPSVEWRQNGIRFVTGVSSGGKPNDDSELYIGYRFENTLAEPL
                     ELEFYLLLRPYQVNPYYQFLNTVGGIGRIVSVREISKGLISVDGKPVRLTEEYESFGA
                     AKFDEGNPVEMIRMGEMPVSSGVTDPSGMAGGIVKYRIKLKAGESKELFAIIPYHSVI
                     RESSGQGAGENPPQVIRAKSSGEGVMPHTLPQTTAEAGEKFFASADYWNKRTGHIRFD
                     LPLSADRLIDTWRSNLAYILINRDRAGIQPGSRSYDRSWIRDGSLTSSALLKSGIVPE
                     VKEFIEWYAAHQYDNGKVPCVVDFRGSDPVPENDSHGQLIYLIREYFNFTADTAFLRS
                     MNYHVIKAADYIGSLVAERSTDHFRNGNDSVRAYYGLVPESISHEGYSAKPMHSYWDN
                     FFIMKGLKDAVEIQDILGDDEHRAKIALMRDEFRKNLYNSIDLAMKIRGIDYIPGCVE
                     LGDFDATSTTIALTPCNELGNLPVPQVYNTFEQYYEFFRKRRDGLLEWVNYTPYENRL
                     IGSFIMLDQPERAHELTKFLLDDQKPHAWNHWAEVVWKDRRFPGFIGDMPHTWCGSDF
                     INAVRSMFVYENEYDHTLVIAAALYRDWIDAPGGMSVSNLPTYYGDISYSVRKEDNTY
                     RFSITGDLNLPAGGIRIRNFNGGAMPSGVTVNGNELTGFTGRDISVTEVPAEVIIRY"
     gene            complement(40037..41416)
                     /locus_tag="PKK03_00750"
     CDS             complement(40037..41416)
                     /locus_tag="PKK03_00750"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNX82946.1"
                     /translation="MKKIYSILLAALVSAACNQPQQTVFRSQGKVDYVLSSADEAMLD
                     SIQKKTFLFFMGEHHPEWGIVKDRTASWAPASIASTGFGIPSFAIGAERGWITRDEAA
                     QITLNMMNFFVNSLQSPDTLASGYKGFYYHFLRMDTGLREWRCELSSVDTGLLMMGIL
                     FARNYYDRENETESQIRSQASRILGNIEWDFMMMPDTGDYARQISMGWHPESGLHNMG
                     WSGYNEALFLYILAAGTGLDDARESYRSWLSSYKWQTPYEGLSHVAFPPLFGHQFSHC
                     FIDFRGLADEYIRDKGIDYFENSRRATYVQRQYAIDNPHGWVGYDSLCWGITASDGPG
                     DKYNFNGKEFLGYAGRGTSGPDYNYFDDGTIAPYGPLSSLPFAPEIVIPTIRSINEKM
                     GDKIWGRYGYYDSFNLTAGWVNDDFIGIDQGPMLIMIENFRTGLVWNYVMKDTVILEG
                     LQTLGFSYL"
     gene            complement(41423..43387)
                     /locus_tag="PKK03_00755"
     CDS             complement(41423..43387)
                     /locus_tag="PKK03_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNX82947.1"
                     /translation="MKRGLTGAWLIILSLLLFSCDRNDPGGEPGLIQLVRAKVGTVYL
                     DLNNPLTDIPVDRNAVIEFSSALDTAAARKSVFLKKGGVTAIPAAITFIDDNRTVVLQ
                     PSSSLDHLSEYTLEITSTLRGASRETFPGVTYSFTTVNGKMLITTVTVNGESFMPPVR
                     PRNVNLAAVSIVVDFSEELDPADYQGYFYFPAPVQYSLSESNRRVTVTTTSGLDYYKK
                     YAFTVTTGLKGVNGYTFDGFSNSFYTGPDLRPKFPLISDDELLDLVQRQTFRYFYDFA
                     HPSSGLARERNTSGDVVTTGGSGFGIMAMVVAMERGFITRAEGLAHMDKILDFLETCD
                     RYHGVWPHWLNGSTGRIVPFSEKDNGGDLVESSFLIQGLITFRQYLDAGVAAEKLIID
                     RINALWQAVEFDFFTQGQNALYWHWSPNYGFQMNMILRGYNETLICYLLGAGSPTHTI
                     SRDTYRYGYMNDGAIMNGNTYYGIMLPMGWAYGGPLFFTHYSFLGLDPRNLQDTWVNY
                     LEQNTAHSLINWKHCETNPFSYVGYSGECWGLTASDNHQGYSAHSPTNDLGVISPTAA
                     ISSLPYTPEQSMNAIRHFYYILGDRLWGEYGFHDAFNPTEGWWATSYLAIDQGPIICM
                     IENHRTGLLWDLFMSAPEVQTGLDKLGFTY"
     gene            complement(43462..44709)
                     /locus_tag="PKK03_00760"
     CDS             complement(43462..44709)
                     /locus_tag="PKK03_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNX82948.1"
                     /translation="MKRKHLFTSLLLFAVAFTLMLTGCKKDPEPLALESLMAGTIDLN
                     GATSPSNVPVNPTIVATFNVDVDPATATASTITMVQDYDDAPVALTINASGNTITITP
                     TANLGTGALYQLSFGAGLKSVDALAITPLQRTFTTAGTFAPAGAVAHFTFEDNANDII
                     GAWDPVAADIIDITYTASRNAAAGKAATFNGTTSLIEIPNGDLLMNTNSFTIAFWVKT
                     NSTGKTNGHFVMGLAGWNGIQYEIYGGYDGSKFAIQYQHATGTASEDMWFPVEAKYNN
                     LPPDQAWQGWTYAKSLTAAEMMAKLKDNWLNVVYTYNAPTKVGTLYFDGVKMKSFDFN
                     LWPDTDAKKGVTGLKYAGIAADKKWALGFIQGRNNRTISDDWANYADPANQHFKGQLD
                     DIRIWHKALTENEITLMYNSEKP"
     gene            complement(44758..46236)
                     /locus_tag="PKK03_00765"
     CDS             complement(44758..46236)
                     /locus_tag="PKK03_00765"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX82949.1"
                     /translation="MKTKITVSLLLVASLVIATGCEDFLQKDPQGELTQEAFPVTASD
                     ALLATNAVYVSVRNWAYHSGGYPIMDIMSDDAHKGSNPNDQLPTVGPFETFTYTTTQD
                     GLDRWWSALYEGIKRANVVIEKVPSITMEEQLRTRYIAEARFLRGLFYFDLVRAFGGV
                     PKVTTTVPATKIPRSTASEIYELVITDLEYAAQYLPEKSLYGPDDAGRATKGAAKSLL
                     ARVYLFLGDYENAEKYALEVIASDEYDLEDNFADANGKNGEHGVESVFEVGAMEVEGA
                     GGNQYANTQGVRGSPNRGWGFNRPSLDLRNSFEPGDPRLDATIIDLGETLDGILIIGD
                     GTTPDEVVVDGTVTEVECYNQKVWYPGTNTITQWGHNRRLIRYADVLLMAAEALNEND
                     KPGDALLYLNEVRERAREGNSSILPDVTVTGKDDLRTAILEERRHELALEGHRFWDLV
                     RTGRAATVLGPMGFETGKHELFPIPQNEIDISQGSLQQNPEW"
     gene            complement(46242..49256)
                     /locus_tag="PKK03_00770"
     CDS             complement(46242..49256)
                     /locus_tag="PKK03_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX82950.1"
                     /translation="MKEIISILFLILTCAGSLSAQATVQGTVVDDQNLPLPGVSVLVK
                     NTFRGTMSDADGTYTISAQPTDTLVFSMVGMVTQQIPVGTKTRIDVILATETTLMDEV
                     VVIGYGTVRKSDLTGSVSSVKTDDLLKVTSLNAEQGLQGKVTGVQVISTSGAPGAGAA
                     VRVRGVGTFNNSSPIFVVDGVILDDISFLNSADIASMEVLKDASATAIYGSRGANGVI
                     IITTKTGIPGQEKASFNISSEFGIQHLAKKIDLLDGREFATISNEIVAGSYNNVDLVP
                     NTDWQDLVFSTAPIHNHQLSVTGATDLTQYYVGIGAFIQDGIIDKSNYNRITLKLNNT
                     YNLTPFLKLGNNLTIAPYSQQNAPNVTYAVYRAQPVLKPYYDSGHYAVVYNVGNPLAS
                     LEYSNDFRKGVRGVGNIFAEATFLKAITLRSSLGIDASYNKSTSFTPAYTVYNPDGTA
                     SQQQNVLSDIYKGTSDNMNWLWENTVSYRNTFADIHSLDMVAGFTMQESSSEFTSIPG
                     ENVLRDGQDFWYISPSYIYDPANNVNTIQSISNGVDAGLYYSMMSYLFRANYTLNNKY
                     IVTVTFRRDGSSKFSKENRFSNFPSVAFGWNVGNEPFMKSVDWISKLKLRASWGIIGN
                     DKISYYDRYSRVQSSIIAVLGNPDAPVPAATYGKSGNPDLKWESTTQTDIGAEVGMLA
                     NRLTTEFDFYNRVTNDILVELSTPGHLGNGQGQRVRYNAASVLNRGFEFNLNWREKVG
                     EVTYSVGLLGSTIHNEVLEIGGNSGIDSTLIGGYLGDGRPVTLSRVGLPIGAFYGYKT
                     DGIFQNDEELAAYPHSSQAGVGDLRFVDVTGEGTLDGRDRTFIGSPIPKFIFGFNGMI
                     EYNGLDLGVDFQGQTGNKIFNGKEVVRPDPYNFEQHVFDRWTGPGTSNTEPRPSFGGY
                     NYTPSDRFIHDGSFIRLRSLTLGYTLPASWSQKAYIQQFRVYLKGTNLLTMTKFTGYT
                     PEIGSYDVLSNGIDYGAYPITSTYSIGVNLQF"
     gene            complement(49466..52264)
                     /locus_tag="PKK03_00775"
     CDS             complement(49466..52264)
                     /locus_tag="PKK03_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNX82951.1"
                     /translation="MKRIMLLTLLLPAALLVYPQGKKAMTIDDLVTWNRISERVISDD
                     GSLMAFRTEPSQGDPVITLYDGAGSLKATFRCATGASVSSDSRFLFFTIKPPEEEVRA
                     LKLKKAKKEDMPLNKLGIYDVTTGVTDTIGRLKSYSAPARWTGWLAWQSEPLKEKPAT
                     TPDTAARGNSNGKGKKPKGESADNGYTLYIRNLTSGRTDKVRFVTEYLFAEEAEKLMY
                     ATTGEDSISEAGVAIIDLKRGEKTVLYSGKAKYKQLTMDKKGERAAFIISFDEKDKAG
                     NTYSLCTWNGKGLATVAASKGTKGIPEGWIINENARLSFAENSPRLFFGTSPEYKIRD
                     TTILEEERANVDVWHWSEGVLHTAQVIRKPREMKRTYTAVFHTDRGKVVQLATAEMPE
                     VQLPVKGDGAKALGMSSLPYELESMWEGQSGSDLWMVDIMTGTTAKIKENLEARPRFS
                     PGGRYLYWYLGADSSWYSFDVTAGKETRLTTPATLRVWDELNDVPDLPGSYPAAGWLK
                     DDRALLVSDRYDIWSLDPAAASPARNVTSNGRKEGIRYRLLDFDPENDYVDPSEKQYL
                     IGVNESTRAEGFYSLDMRRKDVPVRLTGGDYTLGTPVKARKADRLIYTKEDFSIFPDY
                     LLTDLSFRNEIRLTDANPHQKDYLWGTAELVKWTSLDGRPLEGLLYKPENFDPSKKYP
                     MIVNFYEKSSAELNRHRVPEPGRSTIDYHYYTSNGYLIFNPDVYYIDGYPGQSAYNCV
                     MPGIMSLIEKGFVDEKRIGAQGHSWGGYQVAYLATRTNLFAAIESGAPVVNMYSAYGG
                     IRWESGQNRSMQYEHQQSRIGANIWEAPHLYWENSPLFAIEKVETPILIMANDQDGAV
                     PWYQGIEFFIAMRRLGKPAWLLNYNGEPHWAQKLPNRIDFQARMAQFFNHYLKGEAMP
                     PWMKDGIPATEKEFTLGY"
     gene            52643..53623
                     /locus_tag="PKK03_00780"
     CDS             52643..53623
                     /locus_tag="PKK03_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82952.1"
                     /translation="MNRLLLLAGRLSAMILMSVTSLPALAQGTVVTGIVTDHRNIPIV
                     GVRACQVSSTNCTVSDINGVFHLVLDTGKEKILNVTCPGFNPAEAVISDTTTYPVRIT
                     LIPMYVPEGNYVNTDDYSEPGKIIVRSSLNLSLIFSDFSEFSGLLGTYNTDAMDYFSV
                     TGPEIGASFSGFYTGLGIGFGYRYEDEHDSLAIDLNNTLYKLILGYDVVNSSRIRMTP
                     VLSVNWLKFRLQNYPDERRVPLENYLADREIDLRFNQVVAMAGLNLEYKFYSGINGMS
                     DYWSLGIYGGYAVKLNRKPWIRSDGNRITTDNEIRLKPFTAGISVSFYTR"
     gene            53660..54646
                     /locus_tag="PKK03_00785"
     CDS             53660..54646
                     /locus_tag="PKK03_00785"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNX82953.1"
                     /translation="MKYRRLGKTGFEVSEISLGTWQVGGRWGSKFDYTLAESIIREAI
                     DNGVNFIDTADVYSDGESEKAVGRAVRESGAAVRIATKCGRQINPHISKGYTPEVLRG
                     YVEDSLKRVGTDCIDLIQLHCPPTDVYYRPEIFGEFEKLKEEGKILNLGVSVEKVEEA
                     IKAIEYDNVTTVQIIYNIFRQRPRERFFGMAAEKNIGLIIRVPLASGLLSGKFGPDTR
                     FAPGDHRFFNRSGMFFDKGETFSGVPYDAGLRAVSRLKELFPESTPLAHVALNWILMT
                     GEVSCIIPGASSPGQVRSNIEALVFHGFTEEQKRAIQAVYEEDIKPLVHQLW"
     gene            54871..56376
                     /gene="crtI"
                     /locus_tag="PKK03_00790"
     CDS             54871..56376
                     /gene="crtI"
                     /locus_tag="PKK03_00790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HNX82954.1"
                     /translation="MAGNGTALIIGAGIGGITTSLFLAERGYSVDVYEKNVAAGGRCG
                     QMIRDGHRFDMGATMILMPEVYRDILRSLDLTMEDLNLKPLDDIYTLWFDDGTSLAFT
                     TDRNRMEQQLESIEPGSFARAQEYARTGYELYSIAFKKLLGRNFTRFFQFFNLSNALL
                     LIKLKTYLNHYRYTARFFRSEHLRMAFTFQNIYVGQNPFTSPALFSMIPSAELTEGSM
                     FPVGGMYAIAEKLMTRAIEKGVRFHFARPVTQILVSGNRAAGVTLSDGSVAKADVIIA
                     NADLPYIYRELLPHPNKSKRLERMNYSCSALVLHWGLDKQYPLLGHHSTFLSDSFREG
                     LDRIFRDKSMGDDPCFYIHAPVRTDPTAAPEGCDTLSVAVGVGHLDPDRPQDWQRITD
                     KARDAVFRKLRKIGIDDIEPHIKFEIVTSPATWEESLNVTKGSVFGSLGHNIFQMGYF
                     RPHNRDGHYKNLYFTGGSTHPGNGIPMVLMSARLVADRIMNEQKKEPAGKR"
     gene            56373..57296
                     /locus_tag="PKK03_00795"
     CDS             56373..57296
                     /locus_tag="PKK03_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HNX82955.1"
                     /translation="MKTRDQFTALFESIDFEKIIDHPNILIAANFWDNERYCAARTCY
                     RFMRALDDLIDNHKAAHQGISMDDRESFTSEIRHWMGMLDRQMNKSPFNNELGYTVER
                     FRIPLWSLEYFAESMLYDVIHDGFATLDDFIEYSIGASVAPASIFVHLAGLQEHDGVY
                     LDPPFDVRRTAEPCAMFSYFVHIIRDFRKDQLNNLSYFADDVMTRHGLTRTAMSEMAH
                     GAPLTPGFRAMMREYLDIADCYRADTLIIMDEICPKMEPRYRLSLEIIFELYMMVFER
                     IDPERGTFSTEELNPTPDETRERVWRVISRK"
     gene            complement(57516..58202)
                     /locus_tag="PKK03_00800"
     CDS             complement(57516..58202)
                     /locus_tag="PKK03_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNX82956.1"
                     /translation="MKTRILSVIAILVLSVTALQAQVEFGIVAGPNFQNMVGKDADGD
                     KITNGLLVGFHAGVKANIPFAPDFYFQTGLLFSQKGSKNNIGLIPVKSSGDYNTTTRL
                     SYIELPLHLLFRPELGNGHVLVGFGPYVAYGITGSQKTTVGSFSYEPEIKFKNEIDVS
                     DYLTTAYAYYRGFDAGADIFAGYEFGFGLYAQLNAQLGLLNMVTSVQDYDGESNLKNT
                     GFSVSIGYNF"
     gene            complement(58242..58913)
                     /locus_tag="PKK03_00805"
     CDS             complement(58242..58913)
                     /locus_tag="PKK03_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX82957.1"
                     /translation="MRKIKFGVILVLTGSLLLSGCASMNKTTKGAAVGTAAGGAMGAV
                     IGKAAGNTALGAIIGATVGGATGAIIGREMDKQAAEIEQTVPDAKVERVGEGITVEFS
                     SAVLFGFDQSDLSTDAKANLDKLVKVLNTYPDTNIEVQGHTDSKGSETYNQTLSEKRA
                     GSVSTYLTANQINAERITTKGFGETLPEYDNETEEGRAQNRRVEFLITANEKMIAEAA
                     AEAQK"
     gene            complement(58957..>59543)
                     /locus_tag="PKK03_00810"
     CDS             complement(58957..>59543)
                     /locus_tag="PKK03_00810"
                     /inference="COORDINATES: protein motif:HMM:NF017669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNX82958.1"
                     /translation="GVYFITQNPMDVPASVLAQLGLKVQHALRAFTANDRKDIKLTAE
                     NFPLSDYYRTEEAITSLGTGEALVTALNEKGVPTPLAVMMVRAPMSRMDILTPEEIDS
                     INRSSELVTRYSRVVDPESAAEMLEKKIRAAEQRRSDMEARRADEKGYGRSTGRTTGT
                     GSRKRTATHPVVKVLTSATFIRGVMGILTRAMKK"
BASE COUNT        14628 a        15965 c        13959 g        14991 t
ORIGIN      
        1 aattgtagta cattcttatt tgcgaaaaaa cgtacattgt tatttgccgt ttacacatat
       61 tgtatatcaa ggttttatcc taataataag acgtgttttt attaacatat caagcgtaag
      121 atattaacaa tcaatgcgtt gacatttttt gtcatgtact aagctcattt tattatatta
      181 tgttaaggat ctgatcgcag aaagctatat gagccgtata tcgggcatta aacgtgaccc
      241 agtcaaagtg agaagtattt ttcaggtcaa tagcaaggaa tactgcaacg atggtagcct
      301 aaacctctaa tgttaaggat attaaagagt atgacggcgg tgatatcaca tgtccaacct
      361 tgaatgaata tctggatgcc ctgaaacggc tgatgatcat tgaaaaccaa ccggtatgga
      421 gtacacaaat taatttttta agaacacact gattggaaca ctgcctcaac catggatatc
      481 attgataatc aaaggttaat ccatacttat taaatcgtca atagtattga gaaactcttc
      541 aacttttgga ataatagaaa tgatatcctt ttcttcaaag tcataaaaat caccataatc
      601 tcctctttgt ctcagatcga acaaatctcc atatatcatc cctaattcct tatcaagaag
      661 acctggtttt acaaaatgta gtgataattg ggttttgaca cctgaatggg tttgagtgtt
      721 caggttgtgt ttggcaaata atgcaagtac cgaataaaag caggcataat aaagacgatt
      781 catagaagaa ttccagctcc cagtctttgc cagtaattta gcatcttcaa aagttttcca
      841 agctttctct atacgatatc ttatatactc ttcttttgaa gccttcattt ttatatcctt
      901 ataccttcct ttgacacatt cttaaacaga ggcgtcatcc aatacctggt tctccagtct
      961 gccctgttat aaactgtagt agaaaatact tctccggttt ctagttcaag atcgtataat
     1021 ttatgtctga ataatctttc ataattaagg tcaacttcac tatcaacaag aataagaata
     1081 tcccaatcag actcaggatg gtaatctcct ctggctcttg aaccgtagag tataacctct
     1141 gctgatggat ctatctccct aacaagagtc gtaatccttg aagtgatagt tgtattcatt
     1201 gatctcataa aacaaagtta atgaattaag taattggctg aagttgttca aataatctcc
     1261 aaatattaaa taaccagtag tagtgcgtaa ggaaagaatt ttgcctccgg tttccttcag
     1321 atcccccatc tcacgatgaa cacccctgcc ttcagccaac tcttcccact gtcatggcgt
     1381 gttcgggatt ctcaccctat agttatcgcc cattctgggg gtacacacaa ttcatggcaa
     1441 ggaggtgcag ggacacaaaa gggattaact tatgagaaag gcagaacagg ttggaaatac
     1501 tattcatact tatcctctaa tcaattatga accgttccag accgttatta ctaaatgcat
     1561 aaagcctgat acctgataca cagatggttt cgtaccacga atctccgagg ttgagcagcc
     1621 tttggcaaga catatttcta tcgctcacat caataaccag gatatcacta caggatatcc
     1681 tgttccctct attggcatac aacagaagat aaagtttgcc atcattataa caaacatcct
     1741 caacaagaag agtataacca tttggggttg tattctcact gattacagcg agcctctctc
     1801 tgactatcgg gatgcttgaa taatcgagtg tctctagcag ttgacccgaa tggctgtatt
     1861 tctcaattac cggcatatta tccgagactg caaatatgca ggaatcagtc cctgacaggt
     1921 gccggtgatt ccttatcctg gtatgtatat cagtatcaaa aaccttcggt tgaccaaata
     1981 gttctatagc ggaggtttta acatcaaacg aacagagact atttgtttct gacaccgagc
     2041 tcatgaacag cctgttcctg aagaggaaaa aattgaaacc tccactgtaa tctatattat
     2101 caggaagcct gattgtctgc aggtgaccat ctgatctgaa aacctcaaaa ctaccattac
     2161 catcattgta aacccacagt gaatcaccct gaacaatgat gtctgatgct ccaaggaact
     2221 ctcccggccc tttgcccttt gccccaagat accctgccaa ttctccttgc ggagttagtt
     2281 ttattatctg attccgctca taatctgtaa catacaagta tcgatcaaac accattgatc
     2341 tcacatctga caggtacgac gaatcactta aacggtcaat tctcatgtca gatctgaggt
     2401 atgagaccgg catctcctga ttcttacagg agaacacaca tagtgaggat attattaacc
     2461 agaaagttct attcataggc aatcatttct aatttatcag ccatggtagt aaatgtgccc
     2521 gcattcccgg gctccttgga ttggtctgac aacgtaatag catcatttca agctcacttt
     2581 cataattacc gggttgctga attcatctat tgtatccaat attggagcca tttgccgaag
     2641 gtattttgtg tcattccttg ttgcctcgga agcgttgcgg tagtatcttg aaaattcatc
     2701 tccatcaact atactgtaca tgcaattccc ggacgagata aagtatccat tttcatttac
     2761 aggtgcaaat ggtattagat tatcaatatc atttttcaaa taaaccctgg tatacgctct
     2821 tttgaactcc ttcaaagtat tcatttcagt tgcacctgta ttcctgtcat tggtaaccag
     2881 gacctgcgga cccttgtgcg ataccaacat caagaacaaa aaccggtcag tctcgctgaa
     2941 agactgcatc caatatccgg caggcggacc tccggatata tcaaacattg cattatcacg
     3001 ccacacttcc caggccagtt tatcattggc gatataaata atatacctgg gattaacctc
     3061 aaattcccgg ttcgccgtaa agattgtatc actgaatttt tccttgaaaa ggagggtggt
     3121 ttcgtttatc tgataatgaa agtcatacgg aataattgca ggattcctct cactctgatc
     3181 atccttatat tcccggatat tcttgtacaa aacatgcaac gtgtctttct gcatatcata
     3241 acacgagtaa atgtatttaa cccagggctc ttgggcaact gcaacgaata ttgtattatt
     3301 gaaaattcct atacgccatg gaggtaatga ttcttcagga tttattggcc ttgtgatcga
     3361 ggtgacataa ttcccgttga agtcgaatac ttttaacgat ctgtcccagc taaatacata
     3421 aacaaggctg tcttctttac ttattgcata gcagaccggc tgaacctctg ccggaccacg
     3481 ccccttagta tatatcctgt tcaggaattt accgtcgcta ctatatctga aaagatcttt
     3541 ctcaaaatcg acaaagatgc agtcgtcagt aatctcaaaa tcataaacat gcccaatcaa
     3601 cgcctgatca tttctttcga ggggtatata ttcaattctc gtggcgattt cagacagatt
     3661 cattgaatcg cttctggctt tggagagatc aagtttgatc agttttacaa gatctacctg
     3721 agatgaggaa gagtccttgt ttttgcatga cagcaacata aatgtgccgc aggctatcag
     3781 aatgcacaat attctcatat ctctttatgg ttatttccgt aaattcataa agcatagcga
     3841 cagcttatcc aattgcatac tcccaggaac cggaattaaa aatatcatga cagcatcaga
     3901 tcaggtgtct cccggattat tgaaaggact cattaaatgt acgaaaaaaa taaaaatgag
     3961 gcttaaacta tgttttaact ccatactcag agagccctgc cggtgccgac aaacaacccg
     4021 ggaacccgtc atctgtcagg ttcttgccgc caatcagtaa tttccttgcc tcaaagaaat
     4081 aatagttatc tgcaataaaa gcgaatcctg ctatcattat taattcaatc tgaccagaac
     4141 gaggatcgga ttatctgtat ctttcaggtt gtctgccagt ctttcaagct cttctttctt
     4201 ttccgggcat ttgggaatta tatccctgaa ttcactccct gcaatacgag tttttaaagt
     4261 tactggttca accagtccga ccaggtattc tcttccattc tcttcaaaat aaccctttgg
     4321 cagaaacttt gtgccaccat ccagatcatt ttcaataccg ccacttccat tacgctccca
     4381 ataagacaaa taagtatcat ggttatacct gtcaacaagc acgcatccat tcttccctct
     4441 gtaaaacgag taccttattg ttataaaaga gcgcgtctca aaaatctgat cgatagtcat
     4501 atagtcagat agctggctca gagggtcttc tacccttgat ctgggcaatc tgtaatctcc
     4561 aggtttaata ataaactcag ggttgattgt taagtcatca gaaattgaaa aaacagtgtc
     4621 gatgaaatta ctccaataat taagctttga tccgactatg taagtccctc cgccagcaag
     4681 gtaattgctc gagaaaaagt cgtctgttct tctcttgttc attaacagat tgcccgttga
     4741 atcaactaaa acccattcaa attcagcgtc accatactgc atattgtaag tgacaaataa
     4801 ctttgaatca atcagaccaa tcgcgtcaac actcccgccg taactctgaa gggaaatgct
     4861 tctttgaaat gaacctgaac cactatagat tttaattatg ttaccagcat cgcagacata
     4921 tactctaccg gtattctcat cgactgcaaa tttgttcccc cgtacatact cacccggtcc
     4981 tcttcccata gtaccaatta ttccatgtaa ctctccaatt ctgttaaata acatgattcc
     5041 ggtttcatgg tcatataaat aaacaaggtt cccaataaca atgggattat aaaaagaatt
     5101 aatcatcccg attgtaatac tattatcaag agggatatag gttatttcat cggcgatctc
     5161 cgataatgtg atctcttcac cattgaggtt ttcaagatca atcataataa tctcattgtc
     5221 aggcgaaccg catgaaaaca caagaaaaat agtcaggacg aataaggatg ctttcataat
     5281 gataattcct taaatgcgaa ggagtatcag atgacaaggt aatactatca tggcaaaaca
     5341 cagtagagca gcattaaaat caagagggta attattgccg acattaccac tgcaatttga
     5401 ctttcctttc ccattgtacg aatccgtata tcgccattgc gaggaaaatg atgtattgaa
     5461 ctgaaacgag catgattcca cgcattgcgt acatcgcaat actcatgata ttgacaagta
     5521 tccagagtat ccagttctca agtttcagtc gcgcacagaa gagtgatgcc gcgatgctca
     5581 ttacggcgat catggcgtca gtatagataa acgtagccct tacggggaag aagtcaggca
     5641 gccagagatg gatcttgccc atgaccgtgc ccatggccag agccatggca atgatacccg
     5701 caacccagaa tacccgtgcc ttatgactta agagggtcac aggctcgtcg ccgttgctgc
     5761 tcttacttct ccagttgaac cagccgtaga ttgcattgca gaagaaaaag atctgcagca
     5821 ccatagccga atagagattc acctggtagt aaagcatgaa gaagagaacg gcattgacca
     5881 gtgcaaatac ccaggtgatg atttttgccc tggctgcata ccagacggct gccagcccgg
     5941 tgagcacggc aagcagctcg aggagactca tccggtagcc aaggagttcg aagagggtgg
     6001 tgtcgatgtt gaacataggt ctgaaggtct tgcagttttg cggttctgcg gtttgactta
     6061 tatttattat gccggttata aaggtagtga aagcggcgga gacgtgaagt cgaattttgt
     6121 tggtgaaaag tgcaaaacag gtcgaaatat gttgatggta catgcaaatt tcgactgctt
     6181 ttcaatttcg gctcagtttg attagcttta aaccttcatt tggatgcgat gtcaccacgc
     6241 gaagtttatc aatccaggga ggcggagttc agtaccgccg ccgaacagct gcggaaactg
     6301 tctgaccggc tgtcgatggc acggctcaca gcgtttgcag gaggactggt ggtgttcgct
     6361 gtgcttctgt cattttcggt tatcaccgcc tttgccatcc ttactgctgc cctgatcctt
     6421 ttcgcatggc tggtgataaa atacgaaacg acggaaaaaa gcaggaagag attcctgcaa
     6481 ctggcagaga tcaacaggct ggaactgaaa tgcctggatg gcgatttctc cggctttaaa
     6541 accggtgaag aatatgctga aagagaccac ccctatacat atgaccttga tatcttcggg
     6601 aaggcatcac tcttccagtt tctgtgccgt gccaccagca ggccggcctc tgaccggctg
     6661 gcagagcacc tgaaacaacc tgcgggaccc gaagagattc tgctgcgcca gaaggctatc
     6721 gcagaacttc aaccgctgac agactggcgg caggaactga tgaccctcgg atacatgaac
     6781 gccggcgccg gcaatgaccc tggacccttg atgcaatgga ttggaagtga tgatctattc
     6841 cggaaaaccg gtcgggagaa agtcttcacc ggctgtctca gcctcctggc ggtgacagcc
     6901 ataacatcag tcatagccgg gcttccggct gcgatactcg cccctgtgtt cgtggtgaac
     6961 ttcatctttt acttcacacg gtttaggagg atctcgagac tgcaggaaca ggtgagccgg
     7021 tcgtcagacc tgctgaaagg atactccgtc atcatcaaac tgatagaaac ccgcaacttc
     7081 acttctcccc tgctgcagag cctgaagtcg acatttatgg aggagatcgc agcatcggac
     7141 cgtatcaggc aactttcaaa acttgtcggc aggctcgact acaggctgaa cgtgcttgtt
     7201 tcagcgcctc tcaacctgct tttctttgca gacatacact tctgcctcgc cctggagaag
     7261 tggaaacgcg aacatgccct gcgcataccg tcatggtttt cctcgatggc ggaattcgag
     7321 gcgctggctt cacttgcaaa tatggctttc aacaatccag gctgggttta cccgcaagtg
     7381 acggaggact attttgtttt cagggcggag gatatgggcc acccgctaat ccctgcaaac
     7441 agacgtatca acaatagttt cgccacagag ggagccgaaa aagccatcat agtgaccggg
     7501 tcgaacatgt cgggtaaaag taccttccta cgcacctgcg gagtgaatgc agtactggcc
     7561 tttgccgggg caccggtatg cgcctctgct ttcaccatct cgctggtcag gctccattcc
     7621 agcatgagaa tatcagactc gctcgaagag aatatctcat cattttatgc tgagctgcgg
     7681 cgactcaggg ctatcatctc cggagcagaa tccgatccga aggtattcct gctgctcgat
     7741 gagatcctcc ggggcaccaa ttcagacgac aggtataccg gctcggtggc actgataaaa
     7801 caattgaccg gttacggtgc cgtggccatg gtggcaacgc atgaccttcg cctcgcggga
     7861 atggaacagg agctaccgca agccattgag aactaccatt tcgatgttaa agtcaatgat
     7921 gaagaactct tcttcgacta cctgctcacc cctggtatct gctccagctt caacgcctcg
     7981 ctgctgatga agaagatggg aataaaggtc tgatccgcca gtaggcggac aagggtctga
     8041 agatctgatc ctacagtagg cggacaaggg tctgaaggtc tggagtcttc cagtctttgg
     8101 ttctgtttac cttatttatt gattaagaac ctttggagta tttttccatc taattttctt
     8161 tcagcatact gaaagttttc ttttattata taaaaagata ttatattttt gcacataagt
     8221 ttatctgaat caagaatgtt gacatatgat acggcaggat gaaatagctg tggtaataga
     8281 tgcacagaaa gaaacactat taagacagga gccaggtctt agacgtgacg ccctaaccgg
     8341 aattccggtt gcagaatcat tcgccaccat cataaccggc atgcgaagat gcggcaagag
     8401 tacgatcatt atgcaattac tgcacaagaa ccatcaggat acaatatacc tcaatttcga
     8461 cgatattcgc ctgtcaggat ttgaggcagg cgatttttca agattgcata gagaaataga
     8521 aacaagaaca attaagacac tgttctttga cgaaatacaa atagttaacg ggtgggaaaa
     8581 atacattaac cagctcttgc gtgaaggtta caaggtattt ataacaggtt ccaatgcatc
     8641 gatgctgagt gtggaactcg gaacatttct gacaggaagg cacctgtcaa tggagttatt
     8701 ccctttctcc tacaatgagt accttaggtt taaagggctt gacagcgggg agtcatctgt
     8761 cctggactac ctcaaaacag gtggaatacc ggaatatgtc aaaagcgaaa tccccttgat
     8821 tctgaataat cttgtggatg acattcttat gagagacata gctgtcaggc attcagtaag
     8881 ggatttggca tcattgcgtc agctaactgc ttttctgata aacaacattg gaaatctgat
     8941 atcggcaaat aaactggctg gtttatttaa tacaaaatcc ccggctacct tgcttgaata
     9001 cttttcttac cttaaagacg cttaccttgt tgattttatt cctgttttca gtcattcgtt
     9061 aaaagtgcag gcccgtaatc caaagaaagt ctatgtaatg gataccggac tttatacaga
     9121 gaactctctt tcgaccagtg agaatctcgg aagacgtctg gagaatctcg tttttctgca
     9181 tctgcgacgg agataccaac atgttttcta ttacaggaac aatggagaat gtgattttat
     9241 aacacttgag aagaatgtag tgaaagaggc agtgcaggtt tgtctggccg ttaatgaaga
     9301 taattttaag agggaataca atggtttaac ggaggcaatg cacaatcttg gcatcaataa
     9361 tggatgcata gtaactctca accaaaatga catctttgtg gaaaatgatt tgaaaatcag
     9421 aatgatccct gtcgatgagt atctttccgg cggccaggat tgaaagtctg ccactcctca
     9481 acttctttca gtatagtgga agttatcttt tattatactg aaagataaat atggattatg
     9541 ttcagcttgt tgataatggt ctgagctgta attatacgga gttgaatgat tgcgagatat
     9601 tgaacaaaga aatttatcgt aaaaaccaga atatctctta ttatgttctg attatgtatg
     9661 atataactta gcaactatgt aggcggtttt ctccgggtca ctcatactgc cgtctatcca
     9721 gttaatggca atcccccggc gctccatacc gcggaaccag gtcatctggc gcttcgcaaa
     9781 acgatgtatc tcaacctcca gcttctgaac catctcttcc cgcgagagcc tgttcatcag
     9841 gtaaagggtt atatacttgt attccagccc gtaatagata aggtcatcag gatgaatgcc
     9901 ccctaccagc aaccgctgca cctcctcaat catcccctct tcgagccggg agcgcagcct
     9961 ctcgcttatg tgcttccgcc ttgtctcccg gtcaacaaac actgcaatta ccaacggatt
    10021 tcttacaggg aagggtctct catcaactgg atgatgtgaa taatagtctg caatctcaat
    10081 ggcccgtatc gccctcttca ccgtgtcaac atctgtcttg ttgtgcagac ttttgtactg
    10141 cgagagtatc tcagtcagct cctcaagcga ccgccccgca agcagggcac gcaactgtgc
    10201 attgaccggc acctgcacca gccggtaccc gctcacgatg ctgtcaacat acatccccga
    10261 accgccgcag accaccggaa agacgcacct aaccgacata tcttcaaaca ccctcaggaa
    10321 atcgcgctgg tactcatata cattgtacct gtaccccgca tccacgatat caaccatgtg
    10381 caccggcact ctccggccgt caacggtata atccgacagg tccttgcccg tcccgatatc
    10441 catcccgcga taaacctgcc tcgagtcagc actgatcacc tcccctccca tcctgtcagc
    10501 caccatcgcc gccagcctcg tcttccctgt tgcggtaggg ccggtgataa ccaggatatc
    10561 atgcatcgga ttgtccatca gaaattaaag atagccataa tgaggtcacg gtattgcaat
    10621 tttacttaat tttgccgttc ctaaacaaaa agtggtcatg gacttaaggt tcttatacca
    10681 taggacaaaa tatttcataa taaacccagg caaagcctgg gaggtggtcc accgcgagga
    10741 gagaccgatg aggttcgtca ggggcagctt cttcatgccg ctgataattc tagtctctgt
    10801 gagcgcattc cttgggtcga tgttcttcat caacaccacc ctgaaaccga tgtactcggt
    10861 gctggcggcc gccaacacct tcctcttcct ctaccttggc gtttatggct ctgccttcgt
    10921 ggtgaaggag atcatgaagg caatggatct cagccatgat ttcctggtgg cattcaagct
    10981 ggtagcctac tcaatggccc ccatcttcct gtcactcacc gtcagccgcc tgttcgagtc
    11041 gctgcttttc atcaacgtac tggggctcta cggcctatac atcttctggg taggtatgga
    11101 ggtgatggtc aacccgcctg accacaaaaa gctccctatg ataattgcat cggtggtctc
    11161 catgctgata atcttcttcc tcctccaggt catactgtca aagctgactc agaccatcta
    11221 cttttcaata ttcgcctgat atctccgatg agaaatgata atagcaatat cgtcatcaaa
    11281 aaccggaggg cgtcgcacga ttatgaattc ctcgaaaagc tcattgccgg catcaagctt
    11341 accggcaccg agataaaatc gatccgtgcc gggaaagcca acctgaccga ttctttttgt
    11401 gcgtttcgca acggcgaact ctttgtcacc gggatgcata tctcagagta ctactgggga
    11461 aatctcaaca atcatgatcc gttgggtgaa aggaagcttc tgctgacgcg aaaggaactc
    11521 cgtaagtggg aaagaaaggt caatgagacg ggactgacaa tcatcgtcac caaggtgttc
    11581 atcaaccagc gcgggctggc gaaggcggaa atagcccttg caaagggtaa aaaacagtat
    11641 gacaaacgcg acacccttaa gaaacgtgac cacgatcgcg agatggacca ccacgtaaag
    11701 gaacgcggaa ggtaatcaga agttcggact caggagatac ctcgagtaga actggtcaat
    11761 atagcctaca gcttcctcag cctcatcaac tatccggaaa atattcagat cctctggaga
    11821 aatattatgc tcctcgttca gcatcacctc tgtcacccag tcgaacagcc ctgaccagta
    11881 ctttgtacca accagcacta tcgggaagcg cccgatcttc ttcgtctgaa tcagggtgat
    11941 agcctcgaag agctcatcaa atgtcccgaa accaccgggc agcactatga atccctgggc
    12001 atatttcatg aacatcacct tacggacgaa gaaataatcg tatgtgataa gcttgtcgcg
    12061 gtcaatatag gggttggcct tctgctcaaa cggcaggtca atgttgatac cgattgactt
    12121 gccgttgcct ttcttcgcac ccttgttggc tgcttccatg atgcccggac cgccgccggt
    12181 gatgacgccg taccctttct gggtcagctg aaaggcgatc tcctccgcaa gcttgtaata
    12241 cttgttgttg aaatgggtcc tggcagaacc gaagacggcc acgctcggcc ctatcctcga
    12301 aagcttttca aatccttcaa ccagttccgc aataatcttg aaaaccctcc atgaatccat
    12361 cgtgtgaatc tcattccagg ttcgtggctc aaaggcatcc tttatcaggt ccttctcctt
    12421 agcttcgtcc ataggtaaat atttaggcgt taaatatact cttttctaat gttccagcac
    12481 gggtgccaga aactgtcccg tataattatc cttctgctta attatctctt ccggtgtgcc
    12541 cgagaagatg atctctccgc cgccgtgtcc tccttccctg ccgaggtcaa cgatatagtc
    12601 ggccaccttg attacatcca tgttatgttc tatgaccatc accgtatttc cccgttcgac
    12661 cagccttccc agcacctcca gtaaaacctt cacatcatca aaatgaagcc cggttgttgg
    12721 ctcgtcaagt atatacaggg tacgcccggt gtcgcgcctc gagagctccg ttgccagctt
    12781 gacgcgctgt gactcacctc ctgacagcgt cgtcgacggc tgcccgagcc tgacgtagcc
    12841 taggcccaca tcctgcaggg ttttgatctt atggtatatc gaaggaatcc ccttaaagaa
    12901 ctctactgaa tcattgattg tcatctcaag gacatcattg attgaccttc ccttgaattt
    12961 cacctcgagt gtttccctgt tgtatcgttt tccaccgcag tcggggcaca tcacatatac
    13021 atccggcagg aagttcatct ctatggtctt aacccctgca ccctcgcatc cttcgcacct
    13081 gccacccttg atattgaacg agaagcggcc cgggccgaag cccctgacct tcgcgtcagg
    13141 tgtcattgca aacaggttcc ggatctcagt gaaaaccccg gtatatgttg cagggttcga
    13201 ccttggtgtt cgcccgatgg gcgcctggtc aacctcgatc accttgtcga tgttctccag
    13261 tccttcgatg gactcatacg gcagtggcat cctgcccgca ttgtgcagct tcttcgccag
    13321 tgccgggtga agcgtctggt tgatcagcga cgatttgccg ctgccggata cgccggtgat
    13381 gcagataaag gttccaagcg gcaccttcat ggtcaccctc ttcaggttgt tgccggtggc
    13441 gccggtgatc gtgaggaatt taccgttgcc atccctcctc tttgccggga cctctattgt
    13501 ttttgcacca ttcaggtagg cggcagtgag ggtgtcagcc ttaagtatat cttcaggggt
    13561 gccttcggcg acgatcatcc ctccgtgcac gccggcaccc gggccgatgt caatgatatg
    13621 atctgcgctg aggatcatat cacggtcgtg ctccaccact atcaccgagt tgccagcatc
    13681 acggagactg cgcagagcct cgatcaacct gcggttatca cgctggtgca gcccgatgct
    13741 cggttcatca aggatgtaaa gcacgttgac cagcttcgag cctatctggg ttgccagcct
    13801 gatacgctgg ctctcgccgc cggagagtgt gcgggcacca cggttcagcg ccaggtaatc
    13861 gagccccacg tcaagaagga atccaagacg cgagcgtatc tccttcagta tctcggcggc
    13921 aatcttcttc tgccgttccg acatgtcacc cgtgacgttt tccaggtaat ccctgagttc
    13981 ggtaaggtcc atcgcggcaa gctcacctat gttcttcccg ccgatgcgaa accagagagc
    14041 ctccttcttc agcctcatgc cgttgcactc aggacagaca tggtactgga catattgctc
    14101 cctgctcttg cctgtgccgt tggcagtccg gccgctcatt gcctccatgt tacccacata
    14161 gttgacaact ccctcgaagg tcatgaaata gttagatgtg acccccagcg gagtgtttga
    14221 cagtttcagc acctcgtcgg agccgaaaaa tatcttgttc tgaacatcct cgggaatttc
    14281 acccacgggg gtgtcaacgg tgaagccgta cttctcaccg agggcttcga tcatccagaa
    14341 gatccatatg ttccggtatt tgccgagcgg ctctattccg cccttgcgga tgctgagcaa
    14401 gggatcaggc atcatccggc tgatatccac ttcagaaatc tcacccagcc cgttgcactt
    14461 cgggcatgcg ccctgcggcg aattaaatga gaaagtgtgc ggcgcaggct cattgtacga
    14521 gatgccggtg acgggacaca tcaggtggcg gctgtaaaac ctcaggctgt tatcaccggc
    14581 atcaagcaat gccatgatgc cgtcgccctg gtcaagtgcc agcagtaccg catcgtgaag
    14641 cctcttttca gtcacatggc ccacctggaa cttgtctatt accagctcga tgaaatggac
    14701 cttatacctg tcgagcttca gcccctgggt aatctccctg atctcgccgt tgatgcgggc
    14761 ataaagaaat cccttcctcc tcagttggtc gaaaagctcc ttataatggc cctttctgcc
    14821 cttgatcagg ggtgcgagga tgtaaacctt atgctggtca taccgctcag ctacgagatc
    14881 aagtatctga tcatcagtgt atttgaccat tttctcccct gattcatagg agtatgcatc
    14941 agcagcacgc gcatagagca ggcgcaggaa gtcatagatc tccgtgatgg ttcccactgt
    15001 agagcgggga ttcttgttgg tggtcttctg ttcgatggag atgacaggac tcagcccggt
    15061 aatccgatca acgtcaggcc tctccatccc cccgagaaac tggcgggcgt aggctgacag
    15121 cgtctccatg tatcgtcgct gtccttcggc ataaatggtg tcaaaggtaa gtgacgactt
    15181 cccgctgccg ctgagcccgg tgacgaccac cagtttgttt cgcggcagcg taacgctgat
    15241 attgcgaagg ttatggaccc tcgctccata cactcttatc tcgtctttct gttccggcat
    15301 gggaggtgac tataggcccg cctgtatcct tgcacctttc tccggtaccc tgatggtgta
    15361 ttcacgcttc tcgctgttgg tcaggaaggg tctgcgaagc cagggattca ggaatttcag
    15421 tagcttgtag ttggtgtcaa actgccttgc gaactgttca aagctggtaa cggcggtgtc
    15481 aatcttcacc tccctgaacg gtatcggttt gtaaagctca ccggggccga tgttgaagcc
    15541 gtatttggcc ggttctgcca tgaccagttt gagagacacg atacggaata tatatcttgc
    15601 agtctcctca ttcagaagca ggtcgtagta attcgtctcc ttctggatgt caacctgctc
    15661 gctgaggccg ctgaagccgt tgttgtaaga tgctgccgcc attgtccagc tgccgtaccg
    15721 ctcatatgcc ttcaggaaat acttgcaggc gaagcgcgta gatttgtcga ggtcatacct
    15781 ctcatcgatc tccctgttaa tgatcatgcc ggattccttg ccggtgcttt ccattatctg
    15841 ccagtaaccg gtggcgcctg ccggcgagat aacgttgctc aggtcacttt cggcgcatgc
    15901 caggtatttg aaatcatcag gaataccgta ctccttaagt attttttcta tctctgcgaa
    15961 atagcgtccg gagcgcttga tggtaagcag ggttgacccg tggcggtagg cagtagcatt
    16021 gagctcacgg tcgaggctct cgcgggtatc aaaattctgc agtggcagcg cctctccggc
    16081 gaaagttaca ttttcgggga ttgcccagga gactgcaagc ggcgcactct cctcgtaatc
    16141 agtcgcttcc tgctgctgac tgtcggctga aacaagaagg gtaaaaagaa atactgcgct
    16201 gagcagaaac attatggcga tagcggcttt cccggcggta attttcatga tatggcaatc
    16261 tcagattgaa actgcaaaaa tagccaaaat attgcttttg gggaagcaaa taaagcatga
    16321 gaatcaggga tgtcagaatt tgaagaagaa gcccgaggag aagccgacag aataaggttt
    16381 gtagagagaa cctgagattt cactgagagg agtgacatag tacctgaata caggttccag
    16441 gttgaatatt aacttatgtg aaatattgta ttccatgcca aggcccagct ggctgctgag
    16501 gttaaagttg ctcacccctt ccgtgtggcc gatgcgtatc aggtctgatc cttctccggt
    16561 gtatgctgaa ttgtcaacca ggaacccgta agccactcct cctgagacat taagtcccac
    16621 tttgcggtca atcagcttgt acctgatcat aaccggaagc tcaaggtaac ggaaaacctg
    16681 ctgtatatcc gaacccacct gggtcagcct gtacttggaa gggtcagcca tattgccagg
    16741 gataagagtc ccgaccctgg ccttgctgtc tgcaagataa agatcggtat tgcctgccag
    16801 gatcaggccg gcagcggttt caacgctgta gagataactg ctcttgactg aatagtagtc
    16861 agaaagacct gcataaacag ccacatccga gatcgtctgg cctattgatg acagacctat
    16921 gccggactgt atcgagaggc ggtctgaaat cctgtaggct acagagacac cggttgtgta
    16981 agcgggccgc gacttctcac tgttaatcag ctctgccagc ctggcatcat ctcctgaccg
    17041 tgagaagccc attgccggag agagcgatgc tccaagcagg aaacgctggt ctgccttcgc
    17101 acccttcagg tcaggcaggg tggttttgag gttctcccct gctcccttaa agcggccggc
    17161 gttgataatt gtgatctcat ctgaaaagag ctgtaccggc tcatcgggaa gaacatcccg
    17221 ctccggcaga tcagcatatg cgataagctc tgtgggcgcc tcctccatgg ccggcatgtc
    17281 actgagaccg gctggttctg acggggctgt ggtggccgga agatttacta ttctcgcttc
    17341 agcaggcctg gtgaccggcg ctgctttggc aatatcaagg ctcacatcac ggatatctct
    17401 cccgggggca gtaacctctg agagaagcaa cggagtgctg ttattgcgta cataccatgt
    17461 ggccagaaga gaaagggata ccagcgcggc cacaccggca gcaatgccgg aaatgatccg
    17521 cagacgcgag tgtttcaccg gcatcggcgg gatattgtcc cacacgtctg caggcggaag
    17581 aacctcaagg tttttcaggc cgttgcggaa tacaatgtca atatttggct ccctttcaaa
    17641 catgtaccat tctctttgtg acacccgtaa gtatcatcac cttcttctga agaatctccc
    17701 ttgcacgtga gaggtttgat ttgctggtac cttcggagat acccagcatc tctcccacct
    17761 ccttgtgaga atacccttca atggcataga ggttgaatac catcctgtac ttgggcggca
    17821 gctccatgat catgttcagc agatcaaacg cttccagtcc cgcaaagtca tcggtatcag
    17881 gctcggcttc cggttcggta acctcgtcta tgtcatcaac acggttgaga taatacctgt
    17941 tgcggtaccg ctcaagagca gtgttgacga taatcctcct catccatcct tcaaaagaac
    18001 cttcgtggct gaagtgcgac agattttcaa aaatcttaat gaagccctcc tgaagcacat
    18061 ccctcgcctc ttccgtattg cctgcatact gaaggcaaac ggcataaagc ttcggggagt
    18121 acctccggta gagcaactcc tggtcgcgcc tgctgccggt caaacagccg tctattattt
    18181 ccttgtcctt cgccaaaccc ggttattagt cgagttataa aggtaagaaa taatttccgg
    18241 ccttatcaat agattgatga taaaaagggt cgaaaggttg cgttaatggt agtatttaca
    18301 cttaataaag ttacccgtgg atcaattacc ggggcactgc cgtgaccgat ccactgatga
    18361 ctgactgcca gcggatcaat acccggggca ctgttgtgac cggtgccccg atggctgact
    18421 acccgcggat cacttccccg ggaggtgcag tgaccgatgc cccgatgact gcttacccgt
    18481 ggatggcctc accgagtgcc gcggctgcag cctccatgat cccttccgac attgtgggat
    18541 gaggatggat gctggtcata aggtcatggg ctgtggcccc catcttccgt gccgtcacaa
    18601 gcgatgagat catctcggtg acattcattc ctataaggtg accgcctatc agtttgtcat
    18661 cggcagcatt gaacaccagc ttgacaaacc cctcgcgggc gccggccgcg ctggccttgc
    18721 ccgatgccat gaacatgtat ttccctgact tcgtctctat gcctgccgcc tttgctgcct
    18781 cctctgtcat gccgcacgat gccacctccg gggttgtata tgtgcagccg gggatgtttg
    18841 catagtcaac cggatgagga tcccggccag cgatcttttc aacgcatacg atggcttctg
    18901 ccgaggctac atgcgccagc gcgggacccg gtacaatgtc accgatagca tatacgccgc
    18961 tcaccgacgt gcggtaaaac tcatcaacaa caaccctgcc cctgtctgtc ttcactccgg
    19021 cctcctccag gccgatgcct tcaatgttag gagtgatgcc aaccgccgaa agaactattt
    19081 ctgtctccac tgtctcttcg cctttaggcg tttttattgt cacaatacac ttttcgccgc
    19141 tggtgtcaac cttttccacg gttgagttga ccatgatctt catcttcgct tttttgaacg
    19201 cgcgttccag cgtgcgtgag acttcctcgt cctctatcgg aagtacgcgg ggcatgaact
    19261 ccaccagcgt aacctcagtg cccatggccc tgtagaagcc tgcgaactca cttcctatgg
    19321 cgcctgaacc cactaccacc atcgatgcag gcagcgacgg caggttcatc gcttcgcggt
    19381 agccgataat cttcatgcca tcctgccgga ggttcggcag ctctctcgat cttgctcccg
    19441 tagcaatgat gatatgttta gcactgtaac tcaccctcgt cccgtcagct gcctccacct
    19501 ccacggcgcc ggcgcccttc agccggccaa acccgctgat aacaggcaca ttatacttct
    19561 tgaacagata ctgcaccccc ttgctcatcg catcggccac cccccggctg cgggctatca
    19621 ccgccccgaa atcgggcgcc ggcgcctctc cggcatttat gccgtagtcg gcagcatgcc
    19681 gcgtatattc tagcacctga gcactcttca gcagcgcctt ggtaggtata cagccccagt
    19741 taaggcatat cccacccgtc tccgctttct ccaccacagc aaccttcatc ccaagctgcg
    19801 atgcccttac tgctgccaca tatccgccgg gaccgctccc gatgattatc agatcaaatt
    19861 cattcatatt gtagtctttt taagtttttc aatgttgctg ctcctgataa ggcttcgcgc
    19921 accgcgcgcc accagccaga acaacacaag taccgcaaca attgttgcac ctgtcggcag
    19981 gttggtgaac cacgagatga caaaccccgc gctgatccca aaaaagctga ccaccgtgga
    20041 gagcacgatt atccgcaggt accggcgcgt aaaaagcatc actatattag ccggtatggt
    20101 gagcaatgca atgatcatta tcaccccggc catccttatg ttcaaaacaa tggccagtcc
    20161 ggtcaatcca accgagacgt acctgaaaag gtcaacatga cgcgtgaatg tgcgggcata
    20221 attctcatca aaggagatat agagcagtgt ccggtagaaa accgccgtat agactatcag
    20281 tacaaccgag attatcccca gtgcaatcag gtcaccatca gtcacggtca ggatgctccc
    20341 gaaaaggtaa ctcatcaggt ttggcgcata accgggtgta agatatatga aaatgatgcc
    20401 aaccgccatg cccagcgccc agagtatgcc gatggcactg tcctcgcgca tctttccccg
    20461 cgctgacatg tactccatcc ctattgctga tcctagcccg aagacagctg caccaaccac
    20521 cgggtttatc ccaaggtaat agcccagtcc tattcctccg aaggaggcat gtgtcacccc
    20581 gccgctgaga aaaaccagcc tcctggagac aatgtaggtg cccgcaagcc cggccgtgat
    20641 gctggcaaac aatgccccga gaagcgacct gaggacaaat gaatattcaa agaggttttc
    20701 catcagtcat gtttatggag tacaacatgc ggcacatcac cgtgggtgac aagctggatg
    20761 ggacatccgt acatgcggag atcctcgtcg gtgatcttgc tgtgcgggtg gtagaacacc
    20821 tttccgctga cacacgcaaa cgacttcacc caggcggaga tggtacccac atcgtgcgag
    20881 accatcatga tcgtcatccg gttgttaagc agcttcagtt tttcgtagaa gtcggcctca
    20941 aacgaggcgt ctacaaatgt tgccggttca tcaagcagca gcaaccgtgg tgagcctgac
    21001 acggcccttg ccaggtaaac gcgctgcaac tgcccgcccg acagctcatt cagagcctga
    21061 tgtgccagcg gcctcagccc gagctcatcc atcacttcag ccgcccgctt cttttcagtg
    21121 cgacccatgc ggcccatggc gcccctgtca tgcatcatcc ccgaaagcac aacatcctcc
    21181 acagtcaccg gaaagagggt gtcaccctgt gccacctggg gcatgtaacc tatctggttc
    21241 agccgcaggt caccgggtga atagtcgatc cttccggata gcggtttaag caatccgagg
    21301 atcaccttca ggagagttgt cttgccccct ccgttgggac cgatgatgcc gatgaaatcg
    21361 ttctcctcaa cggccatgct gacatcatga aggacgactt tttcctcgta cccggcggtg
    21421 actgaatcta tggtgaataa tatgctcatg gaggaatttg cgatgcgcga tttgagattt
    21481 gcgatgtgcg atgtgcaatg tgcgacttga gttgttttgt ttaatccggc aatcgaaaat
    21541 ctaaaatcga aaatctttgc cgcctactgc tctggcaatc tccatgacag cctccggcca
    21601 gtcactgttc atggggttga tgaccacaag cctggcgccg gtctcacgcg agagcgactc
    21661 tgcattgcgt acatcatact ccgcctgtat gaagataatc tttatctcat tctcccgggc
    21721 aaggtcaatc agttgcttca tcctcgccgg agagggactc ttcccctcat cctcgaatga
    21781 tacctgctca aacccgtaat cacgacccat gtaggccagc gccgggtgat aaatcatcac
    21841 cgtcgggtgc gggccgggtc ccagagccac ccttaccagc gaatcaacct cagatatctc
    21901 ccggttgatc gcataaaggt ttgaatcata tttttctgac gatgctggat tgagtccgac
    21961 gaggaaatca tgcacgtcac gggccattat aaaagcagca gacggcgaca tccagtaatg
    22021 aggatcgggg ccgccatgcg catgaccgtg atcttcatgg tctccttctt catgtccggc
    22081 accctcagac ctcagacctt cagacctcag accttcagat tgaggctccg cacccggcgg
    22141 ggccgaaacc ccgatgcccg aagggtgtcg gggccccagc aacttaatat gccgtgacag
    22201 atccagtact ttcatgtccg gattgacctg cctgaaacgg tccatccatg catgctcgaa
    22261 accaagctga ccgttcatga tgaaagcctc tgaaccggac agggagctta tctgcgccgg
    22321 aagaggttcc catatatggt gatcggcccc cggcggcagc agaacattca ctttaaaatc
    22381 atctcctccg atctgttcaa caaaccaggc gaacggtggt atggttaccg tgaccactct
    22441 gccctcttcc ccccgagggg agcggggacc gcatgaaacc gcggctattg caataaaaac
    22501 cagccacaat gaaattttct tcatgtctct ttttgtgcag tgcaaaatta acaatttatg
    22561 gaatctaagg gtaaaacgat tcagggacga aacagttcaa cagtatgtta tcaatgcacc
    22621 gggaatacgg ctgacaatgc cgcctgtaca aaagaaacag agacaggtgg tgcatctgtc
    22681 tctgtacatt gacccggaga gctgcaatca gtccgtaaac gctcccctct ggtcggaacc
    22741 aattaaaagc agaaaaccaa aaatcagtaa gagttcattg aaatacgcag gtttcccgtc
    22801 gcctccttgg cttttatcct gaatgtccac ttgccgttct tccagccgtt cccctcaccg
    22861 gcctcaaaat tcttcctcca gttcagactg ccgttctcat caagaacaac ttccgaaagc
    22921 tgctttccgt caggcatgat gatagtcacc gccattgatc cctccgcaca atcacctgac
    22981 acagatagat ttacactttt tgcatcatct gcactcatag tcatctcgtt ggtgaaggag
    23041 gcctccatca cctgacgcga ataattccat gatgaaccag gcctgctgcc aagaccctga
    23101 cccttgtaaa ggaatatatt actccctgca tccctgccca ggtcaatata ctcattcaga
    23161 tcattcagtt caggcattac caccacaggc atatcagggt agaggtttcc cgcatgcctg
    23221 ctcatgtcag catactgctg ataataaaac tcaagctcct tattcttttc gacactcttt
    23281 gcatcaatct ccttcatctt ctcctgtatc tccttcctct tggcctcgat gtctgcctgc
    23341 ttccgcttca attcctcagt gagggcctgc tgcttctggt attcggcctg cttctgaagt
    23401 tctgcctgct tctgcagttc ctcctgcttc ttcttttcat ctgcggtttt ggttgatgtt
    23461 tcctggccaa atgcgcccgc tgatgaaagc attattgcca ttacggcgac actgataaaa
    23521 tttactgttc ttttcatgac tgcccattta atgttcgaca atgtaaaatt acatccactt
    23581 ttcacaggct aaggttaatg ggaggttaat gcaaggttaa tcttctccat cttccgccgg
    23641 caatgattaa ttttgaacaa aagtcggtga aatgaagcgt caactccttt ttgctgtttc
    23701 tcttgcagcc ctgcttctct tcgtagcggt tcagctctat atgattcgca atacctggaa
    23761 acagaaggag gagataatca tgatgagata cagtagcctc tcacgggaag gactctccct
    23821 tttactggca aaaaagaaga gtaatggctt cgagaaggcc atggatatta cggataagtt
    23881 cgctgattac ctgatatcag aggagctctg caagctgtat acatcctatg acagcgcctc
    23941 cctcagcaga cttgccctca aggagacata taagataatg gaggagaacg agcagcttac
    24001 ctcattcttc agatcatatt tcggcaatgc cgggtatgac aacgacttca tcccggtaat
    24061 tgccatctcg aaactggtgc tcctggtacc atctgacaaa atggtgatgg ccgatactgt
    24121 ctattccaag ccgggtaaaa cgatggtgct ggtgaacacc ttccgggagg aacacaacaa
    24181 ctttattatc gagtatcatt acctgctgga cctgacacac aagaacgaga tggtcttaag
    24241 ggaagccctc ctctcctttc tgcttataat cggcagtatc ctgattgtct tcctggtgtt
    24301 cggcctgaca tggagaaacc ttctcgagga gcgaaggctc tcggaactga aaagcgactt
    24361 catcaacaat atgacccacg agctcaagac ccctctctcc accattactg tggccgggag
    24421 aacgcttgag aaggaacaaa tcatctcgga ccgggaaaaa gtgcttgaaa cggcacagct
    24481 gataggcaaa cagagcgtgc atctcaacca gcttattaac acaatcctcg aagtaagttt
    24541 ccttgagagg caggaattcg agcttgaccg gcataaggtg gcaattgatg aactgctcca
    24601 ccaggtggtt tcatcatttc ttacatcatg cgacggttgc gccgtgattg aggaggatta
    24661 caccaccaac ggcctggaag cctctgttga catcgtctat ttcaccacca tgatcagcaa
    24721 cctgctctcc aatgcggtga agtactgcga cagggaaccc cttatccgtg taacagccgc
    24781 cgtatcaggg tgtgacgttg aaataacagt ccttgacaac ggagtgggga tcccgcagga
    24841 acacctcggt catgtattcg agaaattcta cagggtaccc caggggaaca tccacaaggc
    24901 aaaggggctg ggactcggac tttattacgt caggcggatt gccatggcgc acggaggcga
    24961 tgtatccgtc accagcaaac ccggccgcgg caccacattc aaaatcagaa taccaatcca
    25021 gaatacgata tgaaactcct gcttgccgaa gacgatatcg atttcgggaa catactgtca
    25081 cagtacctct ccatgagtgg tttcgaggtt acactggcgc gaaacggccg cgaagcatgg
    25141 gacaggtttc acgattcaaa gccggatatt tgcgtgctcg atatcatgat gcccgaaatg
    25201 gacggattta ccctcgcgga gaagctgagg gcgaaggacc cgggaatacc cttcatcttc
    25261 ctgacggcaa aaagcctgag ggaggatatg atcaggggtc tgaagctcgg ggcagatgac
    25321 tatataacca aaccttttga cccggaaatg ctgctgctcc gcataaacaa catcctccgt
    25381 agaatttaca ccgtggctga agacgaatac cggatctctg acaccaccct caggtacaac
    25441 gcccttgagc tcataacccc tgcgggaaag gagaagctta ccctccggga agcccagctg
    25501 atgaggcacc ttatgatgaa caggaacaag gcacttaccc gcgaacagat actgacagaa
    25561 atatggggtg aggacgacta tttcctgggt cggagcatgg atgtgttcat cagccgcctg
    25621 cgaaaataca tagctcccga ccacggatta agcctccgca ccctgagggg gatgggattc
    25681 atactggaag aactgcgaca ataacactga cacccctgac ggcccggaaa cccggtgcca
    25741 cctgacgtgc agcacaccgg tcacaacgaa aaggccggct ccccaggaac ctagtcttcg
    25801 cttatctccg cctcgtgaat tagaaactct gtcaggttga catcatgact gaggttcagt
    25861 tttgatctca gcctgtaacg cgctatctcc accgcccgcg tggatatgtt tgaaagattg
    25921 gcaatctcct tggtggtaaa gttcatcctc aggtacgaac acagtctcag gtcattaggt
    25981 gtcagtgcag gatacctggc cttcagcttt ctgaagaacc cttcctgcga cagcttcagg
    26041 tttttcatca cgttctgcca ctcctccgtc tgggtgtcaa gtccgtgatc aataatctgt
    26101 tccatctccc tgatgaaacg ggcgggcatc tttacagagc tttccttggc cgtctcaagc
    26161 ttgtcacgca gttccctcag ggtgtctgtc tttcttatga ggtaacgcat ggtaagcatc
    26221 agctcataac tcttgtagtc gagctcgctt tccagcctgt tcttgccaac ctcatattca
    26281 atcatacggc ggtggccctc gagtttggaa cggaaaaaca taatcccggc tgcggtaatt
    26341 gctgaggcag caagaaggta gaggaccagg gcccatccgg tcagggcaaa gggcctcctg
    26401 atggtaaaac gggtctccgt catgcccctg ccggctgcct ctcttacctg gagatgatat
    26461 tcaccatgct tcaggtttaa cagtgagaag ttgtcggtcg ccgttcgata ccactcctct
    26521 cccatctcgg tgatcctgta aagatactct cttacttcgc ggtcaaaccc ggatggattt
    26581 gcaatataga cggtcaggtt attcgccctg ctgggcacct gtctttcatc acctgactgc
    26641 gggaacagcg tggcgctcct gttacccccg ctgaaaacca ggcggctgac ggcaagcgca
    26701 gaggcttccg aactgttgac cagtctgtca acgtcataga tgctgaacgc ctgccgtgta
    26761 ggaatgagta gcatgccgga atccagagac atcacctgct gttcccgcca tggcaggtca
    26821 gcatactcat gcacaaattc catgaccttc tcggcctgca tgtcacgggt gatgctgaac
    26881 agggcaatcc tgttgtcaag cacaaaccag taactgttct tctgaaacgg gattatctgc
    26941 gttgctttga caaactcacc caggcccggc tccagcgatg tcacggggaa aaagtccttc
    27001 cgctcatagt caaagccata taaatgatcc gcggcgatga acaccatctg gttgttcatc
    27061 attgccatgg agatgatcct ggcgctgtcg ccggcctggc tgaaatgaag ggaactggtg
    27121 accgaatcag cctcctcgtt cagctccagc cggtataaac ccttctgtgg atgcagcgcc
    27181 cagacgtacc cgaggtaatc aatctccacg aacctggtgg gttcggcata cccttcaatt
    27241 ttggcgcagt actcccattt gccccttgca cccctactga atgagacgag accagtgtat
    27301 gtaccctgaa ccaggtactc accgaacggc cttacagacc atcccccggt gacatccgaa
    27361 atcctgacgg cgttcagccc gtcaataaga aaggtgccgt cattgtgtcc gcacagtatc
    27421 tgcccgtcat attgcaacag cttccagacc tgcccctggg tcccgggtat gatccgaagt
    27481 ccggagaagc ggtacgatcc gttaacattt tctatgtccg tagcaaaaag tccgtggttt
    27541 gtccccagaa aaagataatc ccggtcacgg ataacagtat atattgttcc aaggtcaccg
    27601 cctgagttct cgtagaacgc cacaggtgaa tttgtatcaa gaaaatttgc accatcatca
    27661 agcccgaccc atagcccctt tccggcatcc tggtacagcg acaatacagt attgttgctc
    27721 agcccgttgg agtaatcata ggtttcaaga atcctgccgc tccggtcaca gagcaccagg
    27781 cccctgagaa tggttccgaa ggcaaggagc gaatcattca cagccagccc ggcattgcag
    27841 gtagcctctt tcaggtaccc cgagatctca ttcgccacag gggtgaactt cgctccgtcg
    27901 aacagaaaaa taccgtcatt ggcagtgcag atccacttct catcctggcc taaatcgatc
    27961 agtgcatgaa tcttgtgcgt ggcaaatatc tcgcttccgg ggataaatct gaaaccggtc
    28021 ccgtcaaacc agtaaagtcc ctcatctatt gattgcgcaa taaacctctc gcctgacctg
    28081 aagaagaaca gcatcgagga tggggccgga atcctcttta ccccgttgta gtcataaaca
    28141 tacacagagg tgaacgactg gaaataaagg gtacggccta tctcgaaaat gttccatatc
    28201 tcgtcgttct cggcaacatc ggccgagccg gccagtgagg tgtatgaaag cactccggac
    28261 tgatccttca tccagaaccc gaaatcttca aaggaaccag tgaaaatcac cccgtcaccg
    28321 ttcacatata cagaccttgc tccctggcgg tcgggcacag ggtaacttct cgcagtaata
    28381 ccgttgtatt cgatcaggcc ggcagaattg gcaaaataga cgtaaccgct gaccgggtcc
    28441 ctgcttatct cccagttctg gctcttcacc gggattgttc cgggaataaa ccggctgatg
    28501 tcattcctgc ctccaactgc ctgggcctga acctgcacag gcactgaaag aaaggtgatg
    28561 caaatgacct gaatcagcag tcttagagtt cccccaactg gtatctctga cataagtccc
    28621 gattgttacg caacttcctg ttaccagtgg taaatatagg aaaaagtcag gatctcagag
    28681 gtatatttca accctgtctt cgctgcctga cggcatacct tcaaagacac gctcatccac
    28741 tgccgcgcca ccgggacggt actggttctc aacaggcgtg aacaccacat cacttccgtt
    28801 aacggtaatc cgtttcgggc tgcaacaccc ttcaatgaca tggtaaacaa aggtcacggt
    28861 ccggcccatg attatcatcg aagcctcaag accgtcaaga gaggccggca tcaccgggtc
    28921 aataattaac ctgccccatt ccctcctgat accaaggaga tgtgtcatga tgataccggc
    28981 aaatatgccc gggccacttg aataaacacg ccaccctcct ctcagggtga ttttaccctc
    29041 aattatctca ttatacagct cgtcagcctg gtatcgtgtt tcaaagatgg cgtcagagct
    29101 gctgtaataa cagtttgact gccttatatc agagttggat acaatgtcac gatacccgac
    29161 gggaattgcc tgccgcaatg ccttcatgaa ctctacggca cggccggtgc gcgcaagaga
    29221 ctcggcgtat cttatgtgtt catgaacata catcaggcct atctccctgc cgaagaacgt
    29281 actgctctct gctctccgga aaatcttctc ggggccgccg ctgtatctca gtggccggtc
    29341 catcagccgg gctccgtcag gtccggtgag gtgcctgcag atcagttcca ggtgacttgc
    29401 tgcctgttca ggggtgaaga tgccgctgac aatcccccgc tccatcggca ggaggctgta
    29461 actgatgcct gtcaccgtat ccgacggatg aagcagcagg ctaatcctgc catcatcacc
    29521 ggcgagaccg taacctgcca caacaccgtc tcttaaaaga tacctgttga agtcagtcct
    29581 gatattctct gagcaactgt tcatctcttc ggcaccgtca tgatcgccgg ccaaagcgca
    29641 aacctccgca tactgcctga atgcctggta gttcatctca accgtccatg atgagatcaa
    29701 cctgtcagcc agctccctgt taaccggctg caatgagtca ttccagtcgc ctcctccgta
    29761 acggaccagc gacttacccg gtacaaacga ccctgtcacc tttttaacca gtcggttaat
    29821 atgttctcta agtgtattta tgagattttg cgcttcatta tctttctgaa agtatggcag
    29881 ggatttatca aggaatccaa aatctccggt tgcccttata taactgctca gggcaatgag
    29941 gcaccagtag ataacgtctc cgtgtgcgct gtctgccctt atctcatcgt agctgtcaaa
    30001 catccaccat tgcggccatc cgccgtcagg ctcctggttt gaaaagatga ctgtaagtac
    30061 atttcttgcc tcctcatact tacccatcgc aatcagcagt tcgacaggtc cctgtgatac
    30121 gtcgcgtgtt ccccacgcgg cgccactgaa ctgctcaagg ccgtaagggg tcagaaaatg
    30181 tgtgattgca ttcatcccga accacgggag tatctctgtt atggcactta tatcatcatc
    30241 actgcttctg attgagagtg aacggctcat ttctctccat tccccggagg catcaccggc
    30301 gtctgaagca aactgcctgt ccgcatcgtc aaagaatacc ggctcaattc cggccgtaac
    30361 acagcccgtg aagctcatgc tgaaccgggt cagcggagca gtctctacag taaacaggga
    30421 gttgtcgttc agcacgtcat ctttccctga aaacccgtta ccgccggcac ggtacctgct
    30481 gccggaatac tgaatggtta tcctgtaacg cgcatccggg aacttccggc tgatcatgct
    30541 tccggcagca ggcaatgcga tatactcccc ggcaacagcg tcaggcctga tactccagcc
    30601 gttgagctca tcaaaatcat gggtaaccac cagtgccaca ggatctcctt cgagaacacg
    30661 gaaatccatg ttgacctgcg gcaccttccc ggatgtccac gtctgcacct ggtacagact
    30721 gttgccatgc cggtacagcc acctgcagtg gttaagtccc atctcgaagg ccgatggtac
    30781 gccgagaagc atcctcttcc cccctgccac cacgaaaatc ctttgccccg aaacgggatt
    30841 gatattgaac tggctgttgc atactgacag aagggtgttg aagttggtat taccctgggt
    30901 gatgtgagag ttgaaaaccc cggatgcaaa cgctgtggtt gacattattg actcatccgg
    30961 aagaagatga gccctgttat gcatgatatg cccgtgggga cggtcagcca gcaattcctt
    31021 ccccctgagc atcacgtgcc ggttcatgtc gtaaaagaat gataacaggc ggcctccgga
    31081 aaattcagaa tacctgcgtg gccccggaaa aaaattctca atctccttct cagtcagctc
    31141 ctctgcctgc aagatgaccg gagtgctgaa aaggttcagc tccggcttcc ggaatccttc
    31201 aatcccgctt aagcctcccg gctcgcgaaa ggaactgaag agagcctcag aatgaaggct
    31261 tccggcagcc ccggaatggt catggctgaa ctccattgcc aggcccggca gcctgtcgag
    31321 gtccgcttcc gaggtggccg gtggatgatt atgcaaaaat gtcagagcaa acctgactct
    31381 ccttgtctct cctgccctga gcacaaatgg ctcctcctga atagcaatta cagatgactc
    31441 accggcatac tcacctccca ggctttccgc cagcaatcct tccggaatgc cggtagcccg
    31501 gtaactcttc ccgtaaaact gcattccgtc agtacaggcg gagccggccc ggcccagaga
    31561 ggccatcatc agccacggcc agccacccga ctccttcatg ttctgacggc agcagaccac
    31621 cttcccggcc tcgggatcat caagtatcct cctctctgta tactggctca catagtactc
    31681 atttacaagc ccctgcgaaa cagccttcaa cccggcatcc tgaagacaga caaggtccag
    31741 ggttacctcc ctgccactgt tgttgaacag ttctgtcgtc cattcccagc tcagactctt
    31801 ttctgacagc ctcagactgc atatatatcc gatgtcatcc cagcttccgg caacgctgta
    31861 tatgctgttc tccacacggt aataaccttc actcccggga ccggtaagca gatggctctc
    31921 aattgcgccg tctctctttc tcagaaaaat tccggcatat gcacctgtat aaaccgttgc
    31981 ctggcggaga ctgatcatta tggtgtcagt atgtatgcat gacaccgctc cgttcccgag
    32041 aaactccacc gtcagtcctg cattgttgct gatcttcatt gcctgtttgt ttttatcata
    32101 accatggtgc ccgtccgttt gtcgttcccg gagcccctcc caggatgaac tgctgccgga
    32161 caccgctcaa cccagggtga ttctgagctg aagttcctct ccgtgtctgt ggctgtaggt
    32221 tatcaccatg tgccccccgg tgacaccggt ctcagcaggt ttgccgccga tctccgtcct
    32281 ttttatccgt aaagctccgg gaccctcgag cacggcctct ccggccagat ctctgtcgcc
    32341 gcatagtgtc atgattatct cattaccctc ccttctgaga ccgagaagat cggaagtggt
    32401 atggagcaat gtcactccct ccgccacctg caggcagaca ggggtaagta ttgccctcag
    32461 cggaggccat gaaatttttt ctccgctcag cggcagtctg accgtttcac ccgatgctgg
    32521 atgagtatag gtgacgctgc cgctcatctc ctcgttgtag tagttagcca cgaagagcag
    32581 tgccctgccg tcaccggtga atctctctct ggcaattatg aatccgttgt cagatgcggc
    32641 attccgaagt ctggcaccag atctctccag taacttctca tagaacgccc tgtgagcctc
    32701 agtgtcaaat ccggcccacg tgccgatgaa tgtgaccttt ccttttttca ccctcttttc
    32761 aaagccgcac ggagtgcccc tgagtgtgcg ggcaatgacc tcggcaccgg acagcgattc
    32821 ggaggtgaat acaacctgcg ggttgttgca cttgatgtca tggagatcaa aaaggtctat
    32881 gagcggtgag tctgtcacct cggtccctga cggatctgcc cctattgcat cacgaagcac
    32941 cttgcagggc tgctgagatg tatccctgtc aggaaggtaa ggatagataa ctgcatggcc
    33001 tccgcctgtg atataaccgg cgatggtcat ctgagccggg gcatccattt cgtcggttgc
    33061 aaaaacccag acctgccgat actgcgaaag tgactcaggc gaagcggtgc tgaggtcagc
    33121 catgtcgtaa tcgacgttca gaacctgcag cgccttcaga aggccgtcaa aatatgcagg
    33181 ccgcctgaca gctgatggta tgtaagtcag tccggaagcc tcaccggccg gcctttcaag
    33241 ttccgtggca taccagtgtg gatagaacag cacgcacacc ctggccctgc ggccggaatt
    33301 tacaattatg tctcctgcca cctctgtcat gtggctcatc ttctgcactg caggccaggc
    33361 gcttccgcgt tttccttctg catcgagtgg cgtgaaccag taaaaagtgt ctcctgagta
    33421 acccctgcgg ctgacgttcc ggccctgcga aaacatgtaa aagttccagc ccttcagccc
    33481 gttggcaatg ctggccttat aaaacagctc catctcgcgc gggtttgtca ctacatgata
    33541 ctcccgtgaa ccgctctgaa actctgccgc aaagagcggc ccgcctccct gcattgccag
    33601 cgtgatgtca ttgataatcc tgtcgtcgtg aagattcctg taagagacat actcgggtat
    33661 gtggtcaacg ccgaagatga tctcactccg gcccctgaag aggtcttcat acattgttat
    33721 gttcaccggg aactcgtagc cgttgccgta gatccatccc gggagattat gatacagggg
    33781 caccgtgaca ccgcgttccc ttatcataga gctcagcatc cggaggtaat ctccgtaata
    33841 tcttctccag aagctgtgcc atgcattgtc acgtccgcgg tcagccggag atgcatatgg
    33901 ttcgcggccg tcaggcggca gttcaatctc gtcaaaactg ctgtacgacg tgccccactg
    33961 cctgttaacc tcactgacag taccgtactt ccctgaaacc catgagatga acctgttcct
    34021 gacctcattg ccgtagtcgg cctgccgggc cagccatgaa aagacaccta tctcattgca
    34081 gacctgcatc ataatgacag aacctccggc ctgaatctca tgattcctga tgacgggcat
    34141 gacctggtca taccacaacc tgactttttc aaggtaatcc ttgttgaaga ggctgacacc
    34201 gtcactcatc accctcaccc cccgtcgggt gtgcatccgc accccgtcac cgtatctttc
    34261 gaggaaccag tcgggcagac ccgcacccct gaactctgca agtataaacg gaccgggctt
    34321 gacgatggcg tgtaaaccat gttctttgca cttgcgaagc cagccggtca gatccttttc
    34381 ggggcaggaa ctgccgtcga catcaaacac accctcctcc tcctcatgcc aggcccatgg
    34441 tacatatgtg ctgactgcct tcaacccggc gctgactgct tcatcaagat gcttgtccca
    34501 caacccgcgc ctgatcctga aatagtgtat ctcgccggaa ttgaggaaaa tcctgcgacc
    34561 gccgagataa aaattgttat ccttaacact gaatgtcata tgctgtattt taatcaggtc
    34621 gactgcaatc cttcagcctg accgccattg ttcccgattc ctcttttttc cctgagcagt
    34681 tcctttatct ccatcgtcct tttctccgta aggctgtaac gaaaggcaaa aaccagagcc
    34741 agcaggcaca gaagaacagg caaaccaatc tccaccaccc gcatcataag gagggtgtaa
    34801 ggcgactgtt tcttgagggg gccgactccg gcactgattg gcgcgaggct ggtttcaatg
    34861 acatcaagtg gtgatccggg ctccagggca tagatcatcc tgttcatctc tgctacgctg
    34921 ccgataaggt gttcagtgtg gtcacggctc ttcttctgtt catttgacct ggtgcggagg
    34981 tggaccagca gatcaaagga gctgatcctt gccttagtaa gctttgattg cataattaac
    35041 ggcactgttg accttatcag gctgtcggct gccggagccg gcacacctgc cgtagcagag
    35101 gcaagagcct caatgttcct ttcgagagct gcaatgtttt gcctggtaac tgcatttgca
    35161 ttcatcagca cgttcttcct ttgagcttca atctcaggag taatgacttt cttcctgccc
    35221 ggttttttaa ctgcctcact ctcaagatat accaggtagt ccttcgattc cttgagtgca
    35281 tcagcagcct gtgactgtgc attcttcagc catgtgccgg cagcagcagc atcacccgtg
    35341 taacctttcc agtaggaaac tttagcctgc atatcgcgaa cgcttccctg aaggccgtca
    35401 acctttgtca cctgtgtctc gtcgaaaaga gtgagggtga taagcactcc tgcaacgaag
    35461 ctggcaaatg ccgtaccgag tttaatgaac caccagtaaa tggagtaata gctcccctcg
    35521 gtgcgatgac cggtacgaag ctcatcctca tcgcatatgt cgcccaccat cgaagatccg
    35581 agagtgaaaa agaagagcat gccggcagaa agcattatgg tgggaatgat taccaggtag
    35641 ggatgctcag gattgtagca tacaatcttt gagagctgtg ccagggtcat gagaagcatg
    35701 gagattatca gagtgttccg cttaccgagt ttcgggctga tccagttgag ggggaagacg
    35761 gcaagcacac ccgtgattgc ccagatcgtt ccgtttatcc ccagcagccg cgcaccggca
    35821 accttgtctc ctccaaagac ataaaatatg ggaatgtatg agcccagcag gttcacaaaa
    35881 ttgaaaccca ttgcaagggt gaagatggtc agcaccaggt atatatagtt cttattggtg
    35941 ccgacaacct tcatatcctg ccagaaatgg gttttctcct gcttcgccac cctgacgaat
    36001 cccggttcgc gcagcttcgc agtccaccag aaagagagcg ggatcagaac agctgcgatc
    36061 atcagggaaa cgtaccgcat cccgtcagct tcgttgcctc caggtccccg gaacacttcc
    36121 atattagcca gggcaaatag ccatggagtg ctcatggcga acagattgcc cacgaaactt
    36181 ttcccgctga agaggcgggt gcgttcgtgg gggtcagatg tcatctccat cccgagcgca
    36241 ccatgaggta tctcaaagac ggttgtggcg gtgaagaaga gcagcagggc aaaaagaata
    36301 tagaataact ggaaactctg gaacccggtc tcactgggga accaggcatg cactgtctcg
    36361 cctttgggaa tccaccacat gaccacaaag aagaccgcaa cggctatacc cccgatcagg
    36421 aggaagggtc tgcgcctgcc gaacctgctc cgcgtgttgt ctgacagatg gcctacgatg
    36481 ggatcagaaa acgcatccca gagcctgggg atgatcatta tgagccccag ccagaaagca
    36541 ctgagaccca ggctgatatt tcccatcaac cccaccagct gaccggcgat gttgaacagc
    36601 gccaccggta tgatgccacc tgcagaatag gctgcgagct gaccgaatgt tatccggtca
    36661 tagccctgca ccgcctttcc gcgactcgtc ttcgtatctt ccatcagaat tcctgttatg
    36721 tgctctcaat atctgattat cacctctgcc gggacctctg taacggagat atccctgccg
    36781 gtgaaacctg ttaactcatt tccgtttaca gtcacccctg acggcatggc gcctccgttg
    36841 aagttgcgta tccttatgcc tcctgcgggc aggttgagat cgcccgtaat actgaaccgg
    36901 tacgtgttat cctccttcct gacggaatag gagatatctc cgtaataggt tggcaggttg
    36961 ctgactgaca tacctcccgg ggcatcaatc cagtcgcggt acagggcggc tgcaattacc
    37021 agggtgtgat catactcgtt ctcataaaca aacattgacc tgacggcatt gatgaaatca
    37081 cttccgcacc atgtgtgagg catgtccccg atgaatcccg gaaagcggcg gtctttccat
    37141 actacctccg cccagtggtt ccatgcatgc ggcttctggt catcaagaag aaatttagtc
    37201 agttcatgag ccctctccgg ctggtcaagc ataatgaatg aacctatgag cctgttttca
    37261 taaggtgtgt aattaaccca ctccagcaat ccgtccctcc tcttcctgaa aaattcgtaa
    37321 tactgctcaa aggtgttgta tacctggggc acggggaggt tccccagctc gttgcacggg
    37381 gtaagggcaa tggttgtcga cgtggcgtca aaatccccga gctccacaca tccggggata
    37441 tagtcaatcc cccttatctt catagcgagg tcaatcgagt tgtataggtt cttccggaac
    37501 tcatcgcgca tcagcgctat cttcgcacgg tgctcatcat cacccagtat gtcctgtatc
    37561 tccacggcat ccttcaggcc cttcataatg aagaaattat cccagtatga gtgcatcggc
    37621 ttagccgaat aaccctcatg gctgattgat tcgggcacca gcccgtaata tgcccttacg
    37681 ctgtcattgc cgttcctgaa atgatctgtt gatctctccg cgacaagtga accgatataa
    37741 tcagccgctt tgatgacatg gtaattcatg gacctcagga atgcggtgtc agcagtgaaa
    37801 ttgaaatact ccctgataag atagatcaac tggccgtggc tgtcattttc aggcacgggg
    37861 tcagaccccc ggaagtcgac aacgcatggc acctttccgt tatcatactg gtgtgctgcg
    37921 taccattcaa tgaactcctt cacctccggg acgatgcctg atttgagaag tgctgatgat
    37981 gtgagcgatc cgtcacggat ccagctcctg tcatacgaac gtgaacccgg ctgtatgccc
    38041 gccctgtccc tgttgatcag aatatacgcc aggtttgacc gccatgtgtc aatcagcctg
    38101 tcagccgaaa gagggagatc aaaacggata tgtcctgtcc tcttgttcca gtagtccgct
    38161 gacgcgaaaa acttctcccc ggcttcagca gttgtctgtg gaagagtgtg cggcataacc
    38221 ccctcacccg atgattttgc cctgatgacc tgaggcggat tttcaccggc cccctgcccg
    38281 gatgattctc ggataacgct gtggtatggg attatggcaa ataattcctt cgactcaccc
    38341 gctttaagct ttatcctgta ctttacaatc ccgccagcca tcccggacgg atccgtcaca
    38401 cccgacgaaa caggcatctc ccccatgcgg atcatctcaa ccgggttgcc ctcatcgaac
    38461 ttcgcggcac caaaggattc atactcctca gtcagcctta caggcttacc gtctactgag
    38521 atcagccctt tgctgatctc cctgaccgac actatccttc cgatgccgcc gacggtgttg
    38581 agaaactggt agtatggatt cacctggtac ggacggagca gaagataaaa ttccaactcc
    38641 agcggctctg ccaaagtgtt ttcaaacctg taaccgatgt aaagctccga gtcgtcattg
    38701 ggcttgccgc ccgaggagac accggtcaca aacctgatcc cgttctgcct ccactcaacg
    38761 gagggaagaa aaacataatc accggaatat ccgggaaagc ccataacctg ctccggctgg
    38821 acgttgctgt gattaataag cgtattcccg gttttaagca tgggctccag tgaaaaccgt
    38881 gctttttcag cctccaccat accgtcctcg cttatcaacg cctccttggt atcgctgctg
    38941 accccggtga ctgtccagta tgaggcacgt ccgctgaaat aatctggata acttcctgct
    39001 ggcgagtttc ttgcagcata cattatgaaa tcattggaag tcagtgtact cctaacgtca
    39061 ggaatcttta tctccctgat gccataggac ctgtcgttgg ccggtttcag aaggtcaatc
    39121 cggagataag ccgcctcggc ttccggaagt ctgattatgc tggagccgcc acggttgggg
    39181 ctgaccgaat atgccttttc ccatcttttt ccgtctcctg aaagcaaaac gtcaaattct
    39241 tcggcatgac tgccttcgaa ccatgcgata taaagccccc cgaactcccg cctccccttg
    39301 aaatcaaggg ttatgctctg tttacccgct ttcctgcttt gccagaagga ctcacctgat
    39361 ccgtcagctg caagccccgg tctgtgaccc ctggctgagg aggaagcact catgaccggc
    39421 tcaggccaga catccgtctc cggaggcagg ggctcaaact tcaggtcgtc gagccacagg
    39481 gttcccttcc ctccgacaaa ggaggcaacg gtgaattcaa tcctgtcgac cctcttcata
    39541 tcgtggtcct ccgtcggtcc ccaggcaaaa ctgatgtgcc ttttctttat gcggatcttc
    39601 gtccactcct gtggaaaatc atagttgcgg ttattgaccc accagacatt attcccggtg
    39661 ctgtcaatga attttatctc gaaattgttc gaaggtgatt cggccctgag ccagaaggtg
    39721 aactcgtagt tctcaggcag gtttatcgga aacatcttct gtatgccgcc aaaacctgtt
    39781 cccccggtaa aatcatagtc aatccttacg gcattgccgg ttaccccttt ttcagcggat
    39841 acattcaggg taacaccgtc agagatgttg tatgaccagc ctgaagcctg atcaaaagtc
    39901 tccagtgtac gttgctgggc tgttgcggca aatgatgtca gtgcaatgag caacagcagg
    39961 gcttccgttt ttctggttct gtaattcatg atacgggttt cggttggcgg tttcaggaag
    40021 gattcaggtt aaagcgctac aggtatgaga atccgagcgt ctgaaggcct tccaggatca
    40081 ccgtatcctt catcacgtag ttccatacca gtccggttct gaagttctct atcattataa
    40141 gcatcggacc ctgatcgatg ccgatgaaat catcatttac ccatcctgcg gtaaggttga
    40201 atgaatcata atacccgtac ctgccccaga ttttgtctcc catcttctca tttattgacc
    40261 ttatggtggg gataacaatc tccggtgcaa atggcagaga ggagagcggt ccgtaaggtg
    40321 cgatggtgcc gtcatcaaag tagttgtaat ccggtccgct ggttcctctg ccggcatagc
    40381 ccaggaactc ctttccgttg aagttgtatt tgtcaccggg cccgtcactt gccgtgatgc
    40441 cccagcagag agaatcatac ccgacccatc cgtgaggatt gtcaatcgca tactgccgct
    40501 gaacgtaggt cgcccgacgc gagttctcga aataatctat tcccttgtcg cgaatgtact
    40561 catcggccaa tcccctgaag tcaatgaagc aatgagagaa ctggtggccg aaaagcggag
    40621 gaaaggccac gtgtgaaagc ccctcatacg gagtctgcca tttgtagctg ctgagccatg
    40681 acctgtagct ttccctggca tcatcaagcc ctgttcctgc agcaaggata taaaggaaca
    40741 gcgcctcatt gtaaccgctc catcccatat tatgaagccc tgactcaggg tgccagccca
    40801 tggatatctg ccttgcatag tcacctgtat caggcatcat catgaagtcc cactcaatat
    40861 tcccgagtat ccgtgacgcc tgtgagcgga tctgactttc tgtttcgttt tcacggtcat
    40921 agtaattcct tgcgaagagt atccccatca taagcagccc ggtatccacc gaggagagtt
    40981 cgcacctcca ttcccggagg cctgtatcca tccggagaaa atgatagtaa aatcccttgt
    41041 agccggaagc cagagtatca ggactctgaa gtgaattcac aaaaaaattc atcatgttga
    41101 gggttatctg tgccgcctca tcccgtgtta tccatcccct ttccgcacca atggcgaacg
    41161 aggggatgcc gaacccggtg gatgctatgc ttgccggcgc ccatgatgcg gtgcggtcct
    41221 tgactatgcc ccactccggg tgatgttcac ccatgaaaaa gaggaaagtc tttttctgaa
    41281 tagaatcaag catcgcctca tctgcggatg acagtacata gtcaaccttg ccctgcgagc
    41341 ggaaaactgt ctgctgaggc tgattgcatg cggctgacac aagtgccgcc agtaaaatgg
    41401 agtatatctt tttcataggt tttcagtatg taaatcccag tttatcgagg ccggtctgca
    41461 cttccggggc tgacatgaat aaatcccaga gtagacctgt acgatggttc tcaatcatgc
    41521 agataattgg tccctggtca atggccaggt atgatgttgc ccaccatcct tcagtcggat
    41581 tgaaggcatc atggaaaccg tactcacccc atagccggtc tccgagaatg taataaaaat
    41641 gcctgattgc attcatcgac tgttccggag tatacggcag tgatgatatg gctgccgttg
    41701 gagagatgac tcccagatca tttgtgggag agtgggcgct gtaaccctgg tggttgtcac
    41761 tggccgtcag tccccagcat tcaccgctgt accccacgta actgaacggg tttgtctcgc
    41821 agtgcttcca gttaataagt gaatgggccg tgttctgttc gaggtaattg acccaggtat
    41881 cctgaaggtt tcgcggatca agccccagga acgaataatg ggtgaagaag agcggaccgc
    41941 catatgccca ccccatcggc agcatgattc cgtagtaggt gtttccgttc attatggcgc
    42001 cgtcattcat gtaaccgtac ctgtaagtat ccctgctgat ggtatgagtg ggtgaccctg
    42061 cacccaggag ataacagatc agtgtttcat tgtagccgcg aaggatcata ttcatctgga
    42121 agccgtagtt gggcgaccag tgccagtaaa gcgcgttctg tccctgtgtg aagaaatcaa
    42181 actcaaccgc ctgccagagt gcatttatgc ggtcaatgat cagcttctcg gctgcgacgc
    42241 ccgcatcgag gtactgcctg aaggtgatca gcccctggat gagaaacgaa ctctcaacca
    42301 ggtcaccgcc gttatccttc tcgctgaacg gcacgatacg gccggtggac ccgttcagcc
    42361 agtgcggcca gacaccgtga taacggtcac atgtctcaag aaaatcaaga attttgtcca
    42421 tgtgagcaag gccctccgcc ctggtaatga accccctctc cattgccaca accatggcca
    42481 tgatgccaaa tccggagcct ccggtggtga caacgtcacc cgaagtgttg cgctcccgcg
    42541 caagcccgct ggaaggatgc gcaaagtcat agaaatatct gaaggtctga cgctgaacca
    42601 gatcaagcag ttcatcgtca cttatgagcg gaaacttggg acggaggtca ggaccggtgt
    42661 aaaagctgtt gctgaaaccg tcaaaggtgt acccgttgac acccttcaac ccggtagtaa
    42721 cggtaaaagc atattttttg taatagtcaa gcccggaagt agtcgtgact gtaaccctcc
    42781 ggttgctttc tgaaagggaa tactgaaccg gggccgggaa ataaaaatat ccctggtaat
    42841 cggccggatc cagctcctcc gaaaaatcga caacaatcga tacagctgcc aggtttacat
    42901 tccgtggcct taccggaggc atgaaggact caccgtttac tgtgacagtg gttatcagca
    42961 ttttaccgtt cacggttgtg aaactgtagg tcacccccgg aaaagtttcg cggcttgcgc
    43021 cgcggagtgt tgaagtgatc tcaagggtat attctgaaag atgatcaagg ctggatgaag
    43081 gctgcagtac cactgtcctg ttgtcatcaa tgaaggtaat ggctgcaggt attgccgtca
    43141 cgcccccctt cttcaggaaa acactctttc gtgccgcggc ggtgtcaaga gcgctgctga
    43201 actcaatcac ggcattcctg tctaccggga tatccgtcag cgggttgttc aggtcaaggt
    43261 ataccgttcc cacctttgcc ctcacaagct ggatcagtcc gggttcacct cccggatcat
    43321 tcctgtcaca tgaaaaaagc aacaatgaca aaatgataag ccacgctccc gttaaccctc
    43381 ttttcatgat ggatcgctct ttaaaaaggg agggtccggg ctcttgccgg gaccctcctg
    43441 ttttccgata gaaaattgat attacggctt ctccgagttg tacatgagtg tgatttcatt
    43501 ctcggtaaga gccttgtgcc atatgcggat gtcatcaagc tggcccttga aatgctggtt
    43561 ggcaggatca gcataattag cccagtcatc gctgatggtc ctgttgttcc ttccctgtat
    43621 gaagccaaga gcccattttt tatcagctgc aattcccgca tacttaagcc cggtcacacc
    43681 tttttttgca tcagtgtcag gccagagatt gaagtcgaag ctcttcatct taacaccgtc
    43741 aaaataaaga gtcccgactt tcgtgggagc gttataggta tatacaacat tcagccagtt
    43801 atccttcagt ttggccatca tttcagcagc tgtcaggctc ttggcatatg tccagccctg
    43861 ccatgcctga tcaggaggaa gattgttgta tttagcttct acggggaacc acatatcttc
    43921 agaagcagtt ccggttgcat gctggtactg gattgcaaat tttgaaccat catagccacc
    43981 ataaatctca tactgtatgc cattccagcc tgccagaccc atgacgaagt gaccattggt
    44041 cttgcctgtg gagtttgttt taacccagaa tgcaatcgta aagctgttgg tgttcatcag
    44101 cagatcaccg ttggggatct ctatgaggct ggtggtgccg ttgaatgtgg ctgccttgcc
    44161 tgcagcagca tttcttgaag cagtgtaggt aatgtcaata atgtcagcgg caacaggatc
    44221 ccaggcgccg atgatgtcat tggcattgtc ctcaaaggtg aagtgtgcaa cggcgcctgc
    44281 cggagcaaaa gttccggccg tggtgaaggt cctctgcagc ggggtaatgg caagggcgtc
    44341 aaccgacttc aggccggcgc cgaagctgag ctgataaaga gcccctgtac caaggtttgc
    44401 cgtcggggtg atggtgatgg tgttccctga tgcgttgatt gtcaaagcaa cgggtgcatc
    44461 atcataatcc tgaaccattg ttatggttga ggcagtagca gttgcaggat caacatcaac
    44521 attgaacgtt gcaacaatgg tcggattgac aggaacgtta gatggcgatg tggcaccgtt
    44581 aagatcaatg gtgccggcca tgagtgactc cagtgccagt ggctccggat ccttcttgca
    44641 gcctgtgagc atcagcgtaa atgccacagc aaacagaagc agacttgtaa aaagatgttt
    44701 tcttttcata aaattcaaga tttaaaattc aacattcatt tgtactatca gagttattta
    44761 ccattcggga ttctgctgaa gagatccctg tgatatatct atttcgttct gaggaatggg
    44821 gaacagctca tgcttcccgg tttcgaatcc catcggaccg aggacagttg ctgccctgcc
    44881 tgtgcggacc aggtcccaga acctgtgccc ttccagcgcc agctcatgcc gcctttcttc
    44941 gaggatggca gtcctgagat catccttccc ggttacggta acatcaggaa ggatggaact
    45001 gttaccctcc cttgcgcgct ccctgacctc attcagataa aggagtgcgt caccgggctt
    45061 gtcgttctcg ttcagcgctt cggcagccat cagcagaaca tcggcatagc gtatgagcct
    45121 gcggttgtga ccccactggg ttatggtgtt tgtgcccgga taccatacct tctggttgta
    45181 gcattcaacc tccgttacgg tgccgtcaac caccacctcg tcaggagtgg ttccgtctcc
    45241 aatgataagg attccgtcaa gagtttcgcc cagatcgatg atggttgcat caagacgagg
    45301 atctcccggc tcaaatgagt tgcgcagatc cagcgatggc ctgttgaaac cccatccccg
    45361 gtttggtgat cccctgactc cctgtgtgtt ggcgtactgg tttcctccgg caccctcaac
    45421 ctccatggct cctacctcga acactgattc aacaccgtgc tcaccgttct tgccgtttgc
    45481 atcggcaaaa ttatcctcca ggtcatattc atcagatgca attacttcaa gtgcatactt
    45541 ctcagcattc tcataatcgc ccaggaaaag atacaccctt gcaagaagcg acttggcggc
    45601 accttttgtg gcacgtccgg catcatccgg gccgtagagg ctcttttcag gcagatactg
    45661 tgcggcatat tcgaggtctg ttatgaccag ttcatatatc tcacttgctg tcgatcgggg
    45721 aattttggtg gccgggactg tggtggtaac ctttggcaca cctccgaaag ccctcacgag
    45781 atcaaagtaa aagagtcccc tgaggaacct ggcttcagca atgtacctcg tgcgcaattg
    45841 ttcttccatg gtaatggacg ggactttctc aatgaccaca ttggctctct tgattccctc
    45901 atagagtgcg ctccaccagc ggtcaagacc gtcctgggtg gttgtatagg tgaatgtctc
    45961 aaacggacca actgtaggga gctggtcatt cgggttactg cctttgtgcg catcatctga
    46021 cattatatcc atgattgggt aacctcctga atgatatgcc cagttccgga cagatacata
    46081 aacagcattg gtggccagca acgcatcaga tgccgtcacc gggaatgcct cctgtgtaag
    46141 ctcaccctgc ggatccttct gcaggaaatc ttcacacccc gtggcaatta caagggatgc
    46201 aacaagaaga agtgatacag ttatcttggt tttcatcttt ctcagaattg caggttaaca
    46261 ccaattgaat atgtagaggt tatgggataa gccccgtaat ctatcccgtt cgagagcaca
    46321 tcataacttc cgatctcggg cgtgtatccg gtgaatttcg tcatggtaag gaggttagtt
    46381 cccttcaggt agaccctgaa ctgctgtata taagctttct gtgaccatga tgcaggcagt
    46441 gtgtacccca gggtaaggct tctcagcctg atgaatgagc catcatggat gaacctgtcg
    46501 gaaggggtgt aattatatcc tccgaaagat ggcctgggct ccgtattgct tgttcccggg
    46561 ccggtccacc ggtcaaaaac atgctgctcg aagttgtagg ggtcaggcct gaccacctcc
    46621 ttcccgttga agatcttgtt gcctgtctgt ccctggaaat caacgcccag atccagcccg
    46681 ttgtactcaa tcattccgtt gaacccgaag atgaacttcg gtatcgggga tccgatgaag
    46741 gtccggtcac ggccgtcaag ggttccttca ccggtgacgt ccacaaacct caggtcacca
    46801 acccctgcct gcgatgagtg cgggtatgcc gccagttcct catcattctg gaaaatgcca
    46861 tcagtcttgt aaccatagaa agctccgatc ggcagcccca ccctgctgag ggtcacaggc
    46921 ctgccgtcac ccaggtaacc gccgatgagg gttgagtcta tcccgctgtt tccgccaatc
    46981 tcaagcacct cgttgtgaat cgttgacccg agtaaaccta ccgaataggt tacctcccca
    47041 accttctccc tccagttgag attaaactca aaacccctgt taagcactga agcagcattg
    47101 taacgaaccc tctgcccctg accgttgcca agatgcccgg gtgtggagag ctcaacaagt
    47161 atgtcattcg tcaccctgtt atagaaatca aactctgttg taagcctgtt ggcaagcatg
    47221 ccaacctcag ctccgatatc cgtctgcgtg gtgctctccc acttcaggtc aggattgccg
    47281 ctcttaccgt aggtggcagc gggaacaggt gcatcaggat tacccaggac ggcaatgatt
    47341 gatgactgaa cccttgaata cctgtcataa tacgatatct tgtcatttcc tatgataccc
    47401 cagctggccc tgagcttcag cttcgaaatc cagtcaactg acttcatgaa cggctcattc
    47461 cccacgttcc atccgaaggc aaccgacggg aaatttgaaa aacggttctc cttcgagaac
    47521 ttcgacgatc cgtcacgtcg gaatgtgaca gtaactatgt atttgttgtt gagagtatag
    47581 ttggctctga acaggtatga catcatcgag tagtacagac ctgcatcaac cccgttcgag
    47641 atagactgaa tggtgtttac attgtttgcc ggatcataga tataagaggg actgatgtac
    47701 cagaaatcct gcccgtcacg gagcacgttc tctcccggga tactggtaaa ctcagatgat
    47761 gactcctgca ttgtgaagcc ggctaccata tcaagtgagt ggatgtcggc aaaagtgttc
    47821 ctgtagctga cagtgttctc ccacaaccag ttcatgttgt cagaggtacc cttgtatatg
    47881 tcgctcagca cattctgctg ctgtgaggct gtgccgtcag gattgtagac tgtataggca
    47941 ggtgtgaaac ttgttgattt gttgtacgat gcatcaattc ccaggcttga cctgagtgtt
    48001 attgctttga gaaatgttgc ttccgcgaaa atattaccca cacctcttac acctttcctg
    48061 aaatcattgg agtactccaa tgaagcaagg ggatttccga cgttgtacac aacagcataa
    48121 tgaccggagt cataatatgg cttcagaacc ggctgtgcac ggtacacggc gtatgtaacg
    48181 ttgggagcgt tctgctgcga atagggagca atcgtcaggt tgttcccaag cttcaggaat
    48241 ggcgtaagat tataggtgtt gttcagcttg agggtaatac ggttgtagtt tgacttgtca
    48301 attatgccat cctggatgaa tgccccaatc cctacataat actgagtcag atcagtagcc
    48361 ccggtcactg agagctgatg attgtgaata ggggctgttg aaaagaccag gtcctgccag
    48421 tcagtgttgg ggaccaggtc gacattattg taggaacccg ccactatttc attggagatt
    48481 gttgcaaact cccttccgtc aagcaggtca atctttttcg caagatgctg tatcccgaat
    48541 tcggaactga tattgaagct ggctttctcc tgaccaggta taccggtctt ggtggtgatt
    48601 ataattaccc cgttggcgcc tcttgacccg tatatagccg ttgcggacgc atccttcagc
    48661 acctccatgg acgctatgtc tgcggagttg agaaatgaga tgtcgtcaag gataacgcca
    48721 tctaccacaa agatgggtga agagttgttg aatgttccta ccccgcgaac cctgacagct
    48781 gctccggcac cgggcgctcc gctggtgctg atcacctgga ctcccgttac ctttccctgc
    48841 agcccctgct cggcattcag cgaggttacc ttcagaagat catcggtctt cacagaactc
    48901 accgagccgg tcaggtcact cttccggaca gtaccgtatc ctatcaccac cacctcatcc
    48961 ataagggtgg tctccgttgc cagaataaca tctatcctgg tttttgtccc taccggtatt
    49021 tgctgtgtga ccatcccaac catcgaaaat acaagcgtgt cagtgggctg ggcagaaatt
    49081 gtgtaggttc cgtctgcatc ggacatcgtc ccccggaagg tgttcttgac cagaaccgat
    49141 acccccggaa gaggcagatt ctgatcatca accaccgttc cctgtaccgt tgcctgggca
    49201 gacagactgc ccgcacaggt gaggatcaga aacagaattg agataatttc tttcatgtcg
    49261 gtcagatttt tatccaagtt aagaagtgtc tgccatcaat taaacaaaca gctaagtcgt
    49321 ttgattttct cattactgaa aagggaacag agggatacct atacaatacc tcaacaatca
    49381 catgcaagac attgtatatg aatttattaa aaacttattt cgggaagtgc cttgtaaaaa
    49441 caaatatccc gggaattgac aaccgtcagt accccagcgt gaactccttt tcagttgcag
    49501 gaataccatc cttcatccag ggaggcatag cctcaccttt caggtaatgg ttgaagaact
    49561 gagccatacg agcctggaag tctatgcgat ttggcagctt ctgtgcccag tgcggctctc
    49621 cgttgtagtt gagaagccat gccggcttgc ccaggcgcct catggctatg aagaattcaa
    49681 tcccctggta ccagggaaca gcaccgtcct gatcattggc cattatgaga atgggtgttt
    49741 caaccttctc aatggcaaag agtggcgagt tctcccagta aagatgcgga gcctcccata
    49801 tattcgcccc tatccggctc tgctgatgct catactgcat tgagcggttc tggcccgact
    49861 cccacctgat gcctccatag gcgctgtaca tgttaaccac aggcgcacct gattcaatgg
    49921 cggcaaaaag attcgtcctc gtggcaagat acgccacctg gtaacctccc cagctgtgcc
    49981 cctgggcacc tatccgcttc tcatctacaa atcccttttc aatgagtgac ataattccgg
    50041 gcattacgca attgtaagcg ctctgacccg ggtacccgtc aatgtaatat acgtcgggat
    50101 tgaagatgag atacccgttg cttgtatagt aatgataatc aattgtcgac ctgccgggct
    50161 caggaaccct gtgccggttg agctccgcgg agctcttctc gtagaaattt acaatcatcg
    50221 gatacttctt tgacgggtca aagttctccg gcttgtaaag gagtccctcc aacggccgcc
    50281 cgtcaagcga ggtccacttc accagttccg ccgttcccca caggtaatcc ttttgatgtg
    50341 gattggcatc tgtcagccgt atctcattcc tgaacgaaag gtccgttagc aggtagtcag
    50401 ggaaaatact gaagtcttcc ttcgtgtaga tcaggcggtc agccttcctg gccttgacag
    50461 gggtgccaag ggtataatct ccccctgtaa gcctgaccgg cacatctttc ctgcgcatgt
    50521 caagcgaata gaagccttca gcccgtgtag attcgtttac accaatcaga tactgttttt
    50581 ccgaaggatc aacgtaatca ttctcaggat cgaagtccag aagccggtat ctgatcccct
    50641 ccttccttcc gttcgaagtc acattccggg cagggctggc tgcggccgga tcaagtgacc
    50701 agatatcata tctgtctgat acaaggagag ccctgtcatc cttcagccat cctgctgccg
    50761 ggtatgaccc tggaagatca gggacatcat tcagttcatc ccataccctg agggttgcag
    50821 gggttgtcag ccttgtctcc ttccctgccg tgacatcaaa ggaataccat gagctgtcag
    50881 caccgaggta ccagtagaga tacctgccgc cgggcgaaaa ccgtggccgt gcttcaagat
    50941 tctcctttat cttcgcagta gtgccggtca tgatatccac catccacaga tcgcttccgg
    51001 attggccctc ccacatcgat tcaagttcat agggaagcga tgacattccc agggcttttg
    51061 cgccatcacc ctttaccgga agctgtacct ccggcatctc tgccgtggcc agctgaacca
    51121 ccttacctct gtcagtatga aagacagctg tatacgttct tttcatctcg cgcggcttcc
    51181 ttatgacctg ggcagtatga agaacaccct ctgaccagtg ccatacgtct acatttgccc
    51241 gctcctcctc cagaatggtg gtgtccctga tcttgtattc aggagatgtg ccgaaaaaga
    51301 gtctcggtga gttctctgcg aagctcagcc tggcattctc gttgatgatc cacccttcag
    51361 gaataccttt tgttcccttc gaagccgcaa ccgtagccag tcccttgccg ttccatgtac
    51421 agagtgaata tgtatttccg gctttgtcct tctcgtcaaa cgagataatg aatgcggccc
    51481 tctccccctt tttgtccatg gtcagctgtt tgtacttcgc cttaccggag taaagcacgg
    51541 tcttctcccc tctcttcagg tcgatgattg ccactcccgc ctcacttatg ctgtcttctc
    51601 cggtggtggc atacatcagc ttctccgcct cctcagcgaa gagatattcg gtcacgaacc
    51661 tgaccttatc tgtccggcca ctcgtaagat tcctgatata aagggtgtaa ccgttgtcag
    51721 ccgactcacc tttcggtttc ttaccctttc cgttactgtt gcccctggcg gctgtatccg
    51781 gcgttgtcgc cggcttctcc ttcagcggct cactctgcca tgccagccat cctgtccacc
    51841 tggcaggggc actgtatgac ttcagcctcc cgatagtgtc agtaactccg gtggtaacat
    51901 cgtatatacc aagcttattc agcggcatat cctccttttt cgcctttttg agctttagtg
    51961 ccctcacctc ctcttcgggc ggtttaattg tgaaaaacag aaacctcgaa tcagatgaga
    52021 ctgatgcccc ggtggcgcac ctgaatgtcg ccttcagtga accggctccg tcataaaggg
    52081 taatcaccgg atcaccctgc gagggctccg tcctgaaagc catcagggag ccgtcatcgg
    52141 aaattaccct ctctgaaata cggttccatg tgaccaggtc atcaattgtc atcgcctttt
    52201 tgccctgcgg ataaaccagc aacgcagccg gcagcaacag ggtgagcaac atgattcttt
    52261 tcataccgcg gataatttgt tcatggctta aaaataacaa tttcctgatg ctgcgaaaga
    52321 tgttttacag accttcagat aattggttcc gcctggtgac tccgggatga tccgtgtctg
    52381 tcccgcacgg gcatctcggt tgcgtggctg gagagttgaa ttcaaggttg tcttctgatt
    52441 gctgaatctg atcgaagatt gatttttact tcatgcagcc ccggcaatac agccggggtt
    52501 gcttttttcc cgccattttc aatacatttg agctgcattt ctgaatcccg atcctgattt
    52561 cagcaacaaa gagacatttg ctggcgccgg gggtacaggg aaggaatccg cacctgataa
    52621 accatttact agaagtgaag ctgtgaacag attactttta cttgccggcc ggctctctgc
    52681 catgatcctg atgtcagtga catctctgcc tgcactcgca cagggaaccg tggttaccgg
    52741 tatagtaacc gaccacagga acattccgat agttggtgtc agggcctgcc aggtcagcag
    52801 cacaaactgc actgtttctg acattaacgg cgtatttcac ctcgttctgg atacaggtaa
    52861 ggaaaagata ctcaatgtca cctgccccgg attcaatccg gcagaagcag tgataagtga
    52921 cacgacaacc tatcctgtaa gaatcaccct cattccaatg tatgtaccgg aagggaatta
    52981 cgtaaatacc gatgactatt cggaaccagg aaaaataatc gtccgttcgt cattaaatct
    53041 cagcctgatt ttcagcgact tttcggaatt cagcggattg ctgggaactt acaacacaga
    53101 tgctatggat tatttctcgg taaccggccc tgaaatagga gcctcatttt ccggattcta
    53161 tacaggatta gggatcggtt tcggataccg ctacgaagat gaacatgact cacttgccat
    53221 tgacctgaat aataccctgt acaaactgat attaggctat gatgttgtga actcctcgag
    53281 gataaggatg acacccgtcc tctcggtcaa ctggctgaag ttcaggctgc agaattaccc
    53341 cgacgaacgg cgggtacccc tcgaaaacta cctggcagac agggagatag atctcaggtt
    53401 taaccaggta gttgcaatgg ccggcctgaa cctcgaatac aagttctaca gcggcataaa
    53461 tggaatgagc gattactggt cattgggcat ttacggaggc tatgccgtta aactgaaccg
    53521 gaagccctgg atccggtcag acggaaaccg gatcacaaca gacaatgaga taaggctgaa
    53581 gccattcaca gcaggaatca gtgtatcatt ttacaccagg tgagtaaaca aatcataatg
    53641 gaaattaaaa accggaaaga tgaagtacag gagattggga aaaacaggat ttgaagtttc
    53701 ggagatttct ctcgggacat ggcaggtagg tggcagatgg gggtcgaaat ttgattatac
    53761 ccttgctgaa tcgataatcc gtgaagcaat tgacaacggt gtcaacttta ttgacactgc
    53821 agacgtatac tccgacggag agagcgaaaa agccgtgggc agagccgtgc gtgaatcggg
    53881 cgccgcggtg cgcattgcta cgaaatgcgg caggcagata aacccgcata taagcaaggg
    53941 atatactccc gaagtactcc gcggctacgt cgaggactca ctgaaaaggg tcggcacaga
    54001 ttgcatcgac ctgatccagc tgcactgtcc tcctacggac gtctattacc ggccggagat
    54061 attcggcgaa tttgaaaagc tgaaggagga aggcaaaatc ctgaacctcg gcgtcagcgt
    54121 cgaaaaggtt gaggaagcaa tcaaggccat cgaatacgat aacgtcacca ccgtccagat
    54181 aatatataac atcttccgcc agcggccccg cgaaaggttc ttcgggatgg ccgccgaaaa
    54241 gaacatcgga ctgatcattc gtgttccgct ggcaagcggc ctgttgtccg ggaaattcgg
    54301 ccctgacacc cgtttcgccc ccggcgatca ccgtttcttt aaccgaagcg gtatgttctt
    54361 cgacaagggg gaaaccttct ctggagtgcc atatgatgcc ggtctccggg ctgtgtcaag
    54421 gctgaaggag cttttcccgg agagcacccc gttggctcat gtggccctga actggatact
    54481 catgaccggg gaggtcagct gcatcatacc cggggcttcc tctcccggac aggtgagatc
    54541 aaacattgaa gcactggtat tccacggttt cacggaagaa cagaaaagag caatccaggc
    54601 ggtctatgag gaggatataa agcccctggt acatcagctc tggtagccgc aatggcagac
    54661 atgacccatg ccaatgccac ctgcagcaac cgaacaaccg ggtctgtgaa tgatatccga
    54721 agggagtttt tccgccactc acctgccata taccgcagtt gacccttatt aaacaggtgt
    54781 tattcgcccg tttgttaata tatttgtttt ctgattgcct aaaaggcgtc atgaaacggg
    54841 aacaaaacac cttattaaat tttatacctg atggctggta acggaacggc gctgataatc
    54901 ggcgccggga ttgggggcat aaccacgtca ttattcctcg cggaacgtgg ctatagcgtc
    54961 gatgtatatg aaaaaaacgt cgccgcaggc ggaagatgcg gacagatgat tcgcgacggg
    55021 caccgttttg acatgggcgc cacaatgatt cttatgccgg aggtataccg cgatattctc
    55081 cggtcactgg acctgaccat ggaggacctg aatctcaaac cgctcgatga tatctacaca
    55141 ctctggtttg acgacggaac aagccttgcc tttacaaccg accgcaatcg gatggagcag
    55201 caactcgaga gtattgagcc gggcagtttc gcaagggcac aggaatatgc cagaacaggt
    55261 tatgaactct atagcattgc ctttaaaaaa ctcctcggaa gaaactttac ccgcttcttt
    55321 cagtttttca atctcagcaa cgcccttctg ctcatcaaac tgaagacata tctcaatcac
    55381 taccgctaca ccgcccgctt tttcaggtca gaacacctcc ggatggcatt tacgttccag
    55441 aatatatacg tcggtcagaa cccgttcacc tctcctgccc tcttctccat gatcccttcc
    55501 gccgaactca cggaaggctc gatgtttccg gtcggaggaa tgtatgccat tgctgaaaaa
    55561 cttatgacaa gggccatcga aaagggcgtc aggttccatt tcgcaagacc ggttacccag
    55621 atcctcgtct ccgggaacag ggccgccggt gtgactctca gcgacggaag cgtggcaaaa
    55681 gccgatgtga tcattgccaa tgccgacctg ccatacatct accgcgaact gctgccacac
    55741 ccgaacaagt cgaagcgcct ggaaaggatg aattactcat gttccgcact ggttctgcac
    55801 tggggactcg ataaacaata ccccctactc gggcatcaca gcacattcct gtctgacagc
    55861 tttcgcgaag gcctggacag gatattcagg gacaagtcaa tgggcgatga cccctgcttc
    55921 tatatccacg ccccggtacg cactgatccc actgccgcac cggaaggttg tgacaccctc
    55981 tccgtagccg tgggcgtcgg gcacctcgat ccggaccgtc cccaggactg gcagcggata
    56041 accgacaagg cccgtgacgc ggtcttcagg aaactgagaa agatcggtat tgacgacata
    56101 gagccacata tcaaattcga gatcgtcacc agcccggcca catgggagga atccctcaac
    56161 gtgacgaagg ggtcagtgtt cggaagcctc ggccataaca tcttccagat gggttacttc
    56221 aggcctcaca acagggacgg acattataaa aacctgtatt tcacaggcgg cagcacccat
    56281 ccgggaaacg gtataccaat ggtgctcatg tccgcaagac tggttgccga caggatcatg
    56341 aacgaacaaa agaaagagcc ggcagggaaa agatgaagac gagggatcaa ttcacggcat
    56401 tgtttgagtc tatagatttc gaaaagatca tagaccaccc aaatatactt attgcggcaa
    56461 acttctggga caacgagagg tactgcgctg cacgcacctg ctaccgtttc atgcgggcgc
    56521 tagatgacct gattgacaac cataaggctg ctcaccaggg aatcagcatg gatgacaggg
    56581 agagctttac ttcagagatc aggcattgga tgggtatgct tgacaggcag atgaacaaat
    56641 cacctttcaa caatgaactg ggttacacgg ttgagagatt caggataccc ctgtggtcac
    56701 ttgaatactt cgctgaatca atgctctatg atgttattca tgacggattt gccacacttg
    56761 atgacttcat tgaatactca ataggcgcat cggtggcgcc ggcatcgata tttgttcacc
    56821 ttgccggcct gcaggagcat gatggcgttt accttgatcc gccgtttgac gtacgccgta
    56881 ctgccgaacc ttgcgcaatg ttcagttact tcgttcatat tatccgcgac ttccgtaagg
    56941 accagcttaa taacctcagc tactttgccg atgatgtcat gacccgtcat gggctcaccc
    57001 ggactgccat gagcgaaatg gctcacggcg ctcccctgac acccggattc agggccatga
    57061 tgcgtgaata ccttgatatt gcagactgtt accgggctga caccctgata attatggatg
    57121 agatatgtcc gaagatggaa cccaggtacc gcttaagcct cgaaatcatc ttcgaactct
    57181 acatgatggt cttcgaacgt attgaccctg aacggggtac cttctccacg gaagaactta
    57241 atcccacccc tgacgagacc agggagaggg tctggagggt catttcaaga aagtagtcgg
    57301 cagtcggcag atatgcgatt tgcgatttgt gatgtgggcg atgcccaaag ggcatcaagg
    57361 attgtagaag tgcgatttgc gatattcccc gcagggaatt aagcattgta gaactgaaca
    57421 ctaatggatg gcgatggtcg tagaccatca agcattgtaa aaggccgcct gccacacggc
    57481 agacggcccc taagacatga tctgtaactg accggctaga aattataacc tatcgaaaca
    57541 ctgaacccgg tgttcttgag gtttgattca ccgtcataat cctgaacact ggtaaccatg
    57601 ttcagtaatc cgagctgcgc attaagctga gcatacagtc cgaaaccaaa ctcataccct
    57661 gcgaaaatgt ctgcaccggc atcaaaacca cgataataag cataagcagt agtcaggtaa
    57721 tcagaaacgt caatctcatt tttaaacttg atctcaggct cgtatgaaaa actgcctaca
    57781 gtggtcttct ggctaccggt tattccgtaa gcgacataag gaccgaaccc gaccagaaca
    57841 tgcccgttgc ccagttcggg cctgaacagc aaatgaaggg gcagttcaat gtaagaaagg
    57901 cgggtggtgg tgttatagtc acctgatgac tttacaggaa tcagaccgat attgttcttc
    57961 gaacccttct gtgaaaaaag cagtccggtc tgaaaataaa aatcaggggc aaatggtatg
    58021 ttcgccttta ctccggcatg aaaccctacc agcagtccgt tggtgatctt gtcgccatcg
    58081 gcgtctttcc ccaccatgtt ctggaagttc ggaccggcaa caataccaaa ttcaacctgc
    58141 gcctggagcg cggtaactga taaaactaaa attgcaatga cagatagaat cctggttttc
    58201 ataatttaac tatttggtta ataaaaactc ttcctttgat tttacttctg agcttctgct
    58261 gctgcctcgg caatcatctt ctcatttgca gtgatcagga actcgaccct gcggttctga
    58321 gccctgccct cttctgtctc attgtcatat tcaggcaagg tttcaccgaa tcccttcgtg
    58381 gtgatacgct cagcgttaat ctgattggct gtcaggtatg ttgacactga accggctctc
    58441 ttctcagaaa gggtctggtt gtatgtttca ctccccttgc tgtcagtatg gccctgcacc
    58501 tcgatattgg tgtcggggta tgtattaagt accttaacca gtttgtcaag attggctttt
    58561 gcatctgtgg agaggtcgga ctgatcgaag ccgaacagca ctgcgctgct gaattcaacc
    58621 gtaatacctt cgccaacgcg ctcaaccttg gcatcaggta cggtctgctc tatctcagca
    58681 gcctgcttgt ccatttcacg gccgattatg gctccggtgg cgccgccaac cgttgcaccg
    58741 ataattgcgc cgagagcagt attgcctgct gccttcccta tcacggcacc cattgcaccg
    58801 ccagccgcag taccaactgc agcacccttt gttgtcttgt tcattgaagc acatcctgaa
    58861 agaagaagtg atccggttag aactagaatt actccgaatt tgatttttct catgtttctc
    58921 attttagatt caataattat caattacaat tcttttctac tttttcattg ccctggtaag
    58981 tatccccatc accccgcgga tgaatgttgc ggaggtcagt acctttacca ccggatgggt
    59041 tgcggtcctc ttcctggatc ctgttccggt tgtccttcct gttgaccgcc cgtaaccctt
    59101 ttcatcagca cgccgcgctt ccatatcgct ccttctctgc tctgccgccc ggatcttctt
    59161 ttccagcatc tctgcggcgc tctccggatc aaccacccgc gaataccttg tcaccagttc
    59221 tgacgaccgg ttgattgagt ctatctcttc cggcgtcagg atatccatcc tgctcatcgg
    59281 ggcacgcacc atcatcactg ccaggggagt gggcacaccc ttttcgttca gcgctgtcac
    59341 cagcgcctca ccggtaccca gggaagtaat tgcctcctca gtcctgtagt aatcggaaag
    59401 cgggaaattt tcagcagtaa gcttgatatc cttcctgtca ttggcagtga aggcacgaag
    59461 cgcatgctgc accttcaggc caagctgtgc cagcaccgaa gccggcacat ccatcgggtt
    59521 ctgggtgatg aagtaaactc cca
//
LOCUS       DAOPXU010000004        51320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_516_length_51320_cov_13.583302, whole genome shotgun sequence.
ACCESSION   DAOPXU010000004 DAOPXU010000000
VERSION     DAOPXU010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 51320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51320
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_516_length_51320_cov_13.583302"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..731)
                     /locus_tag="PKK03_00815"
     CDS             complement(87..731)
                     /locus_tag="PKK03_00815"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNX82959.1"
                     /translation="MEKIKYNYIVIEGNIGAGKTTLASMIARDYNAKLILERFADNPF
                     LPKFYNDPSRYSFPLELSFLADRYRQLKEELVEPDLFKSFTVADYYFMKSLVFSSKTL
                     EQDEFSLYRQIFYIIYSSLPKPDLYVYLHVPPERLLKNIAMRGREYEKSITADYLSGI
                     QESYFTFFRQNKENRYLVLDVSNIDFVACHDDYLKVKEMIFTEPVAQGINLRNF"
     gene            complement(899..3154)
                     /gene="pnp"
                     /locus_tag="PKK03_00820"
     CDS             complement(899..3154)
                     /gene="pnp"
                     /locus_tag="PKK03_00820"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNX82960.1"
                     /translation="MYKLIEKSIDLGDGRSIVIETGKLAKQADGAVVVRMGKTMLLAT
                     VVAAKDAKEDTDFMPLSVEYKEKYAASGRFPGGFLKREARPGDNEILISRLVDRALRP
                     LFPDDYHAEVFVTINLISAEKDIMPDALAGLAASAALAVSDIPFHGPISEVRVSRVNG
                     EFIVNPTFEQVETADLDIIVGATYENIMMVEGEMKEVSEDVMLEALRIAHEAIKPQCV
                     AQKELSELVGKSVKREYCHEKNDEELRKKIWDETYDRCYKLAASGSDKKTRTDGFDAI
                     KAEFLSQFPEDAPADEMLVNRYFHDVEKEAARRLVLDEHRRLDGRKLDEIRPIWCEID
                     PLPAAHGSAIFTRGETQSLTTVTLGTKLDEKMIDEVLKQGSEKFTLHYNFPPFATGEA
                     KAARGTSRREIGHGNLAHRALKNMMPPEETNPYAIRVVSDILESNGSSSMATVCAGTL
                     ALMDAGIKIAKPVSGIAMGLITDKDGKKFAILSDILGDEDHLGDMDFKVTGTRDGITA
                     TQMDIKVDGLSFEVLKQALEQARRGRMHILGIMEQTIAEPRPDLKPHAPRIEKIYIPK
                     EMIGAVIGPGGKIIQDIQATTGATIVIEEINGQGMVDVFGDNYEVIRAALDRINAICA
                     VPEVGKVYTGKVKTITQFGAFVEILPNKDGLLHISEMDWKKVGTVEELFKEGDIVEVQ
                     LIEVDQKTGKLRLSRKPLIPKPEGWVEPPKREHTERRDYQGSRDGNRSDRGDRGNREH
                     RSHDRRRDNNR"
     gene            complement(3245..3514)
                     /gene="rpsO"
                     /locus_tag="PKK03_00825"
     CDS             complement(3245..3514)
                     /gene="rpsO"
                     /locus_tag="PKK03_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNX82961.1"
                     /translation="MYLTTEKKQEFFKTFGSSELDTGSAEGQIALFSYRISHLTEHLK
                     KNKKDFSTQRALTTLVGKRRHLLDYLKVKDISRYREIIKALNLRK"
     gene            complement(3743..5065)
                     /locus_tag="PKK03_00830"
     CDS             complement(3743..5065)
                     /locus_tag="PKK03_00830"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNX82962.1"
                     /translation="MKEEFLHWLWKNLFFEGGSLCDREAGPIEVITPGEYNRDSGPDF
                     FNTRLNIGGTEWAGNTEIHVNASDWYRHGHHTDHAYDNVILHLVYNEDADVYTASGRR
                     LITARPVFDPVLWENYLDFMNNPSPLPCSGLIGFSDGFRIKHWLWSVAVERLERKHGE
                     IREILAKTGNDWEETLYRLVTRYFGFRVNTDPFEILAARLPLKIIRKHADQLMQVEAL
                     LYGTAGLLDGALFREAVSDGYYLLLSREYRVLRAKYSLQPVDGWLWKFHRLRPANFPT
                     VRLSQLAALLSHSDGLFSRVLGCSDRESLRALLSVSASSYWNSHYQFGREAPPVAGRA
                     GKQSVDLLIINAIAPLLFVYGKVRQQQEWCDRAVEILDSLPPEENSVVTDFARAGLKP
                     ESAFASQALLELRNLRCRYHRCLDCTIGSSLIAMGQKIRRSDSLFLEP"
     gene            5272..6588
                     /locus_tag="PKK03_00835"
     CDS             5272..6588
                     /locus_tag="PKK03_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX82963.1"
                     /translation="MKTLTKALAVLVLFSMAINAEAQIKVDLKKKLEQQVNRRLNQKA
                     DQAIDKALDTVEDSIRGDVKKDGNAGGNATGQNSGNAQPQGKSGQNADAQSGGQQEPA
                     FQSYSKYDFIPGEKVIFYDDFSQDAVGDFPALWNTNGSAEVVTTNLFPGKWMRFVMNQ
                     CVWTDELLKLPENYTIEYDVIPQGGLEGEGMSGWHMKLMQAKNAKAWDGGTQPGNGGF
                     EFRIEYFGRPSYHTWLYGQECSVSNLSGYVEGEQYKQKINKLYHIAIWIQKTRIRVYQ
                     DQNKIIDLPRAFPAGCVKPDRIRFEYGAAMISNIRIAVGAPDMRNKLITEGKLVTYGI
                     YFDVNKDVVKPESYGTLKEIATVLQENPDVKVKIVGHTDSDGADASNLDLSKRRGASV
                     KNELVKNFGIEASRLESDGMGESQPVAPNDTPANKALNRRVELIKL"
     gene            6644..7639
                     /locus_tag="PKK03_00840"
     CDS             6644..7639
                     /locus_tag="PKK03_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82964.1"
                     /translation="MKRILLILVAAGIVLTGAAQTRTQEYMKKIPALPKDSCNATRSS
                     VEGFVSQVSALRDELVTEIEGIREAVDSHMESNADVAEANAMKQMAQMYGMSQADINK
                     LKNAKDMSDADKQALANKMVMQQANMSMDEAKNLSKMSEAGRKAYAEAYAVEAMATAQ
                     NDPNQEAKSNNAKNMYQSGVAQQAAISKINEITTRIAALYSPIEKDPERQKMLDRMKA
                     WNNQLTSMMGIVSDGEARIMDSLSLRIQNEQIAYCNKYTPQYRAALRKHLQTVKASMP
                     DYKKFGDINAETTKIQTGIDMPEEGKELASLEAIADYLRALEEVYRYKLYYSDEY"
     gene            7693..9801
                     /locus_tag="PKK03_00845"
     CDS             7693..9801
                     /locus_tag="PKK03_00845"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX82965.1"
                     /translation="MRHILVLLMLSVSLLMSGQTGQSTDQIRQRMAEIRRTTNWDDPA
                     AAAKANAEIKKLAAQMGGGQPPVSFGSGQAGKAEPATPETIAVKSSDVTTGNIAAIAQ
                     RFYSRSYKTLDAAGKTQFDQHFREAAADDFTFRSVRKLSGIGGVLISMGDDHNLACVY
                     LAAAVKAMPDDTLSVNNFGAYLRIIDSVKASIPVLLYADKLYGESPIVLTQLGCSYLE
                     LGHEKEAESYLKEALEYNPGFGQAHTALCELYIRQGRLEDALVELFAGVKGMGASYAR
                     ASGNYAYLQQQAEKAGENGNQSAKEAFWDETRNQISPDDALAPLVPSVDRLKMPSFPT
                     CQKITDWMEGGGYGTSVQTYNRMHSLLMKFSEELLQVHKELPELPPNAVLRDYPNERF
                     ALDCITEYFFRESEEEAHDFNDAYDAIMDEVNAEAEAYLQNKETYTKEYVRCAEGCGG
                     DGYCIEECHRVYCTKECPAANIYNKSLQGHWEDYLYQFNETVDRQKEILNDLYEFSAR
                     WFSKIESPYWSKIYAYEIQRVALSAIMNVYSAHARPFQWPAHNDCGTDCSAYANPAPP
                     PPEDAEEKEPKGNECPEDKKLNIGLGICSIALDCESAEFGCSAGAAVSIKRNFKNKST
                     STFLGVGAEAGLGFAHAEANAGVTMTRHDNGDLDVGVKAELTGTVGVGPGSTGKNYEM
                     NVTVMEGLKTETKDVIGIGF"
     gene            9852..10838
                     /locus_tag="PKK03_00850"
     CDS             9852..10838
                     /locus_tag="PKK03_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82966.1"
                     /translation="MKKTLFCMLMLAAALSSPAQDNASLADRPGTFEILSRTDYAWHE
                     CGFTQADMTANLERIKDVIEVVRQNPVLSDIKGFAARARLHTMSMTCKREVWYGVPVR
                     VAFEICTFFYNKEGKVVYNTMEPPSWSLCTNDFIPGWSDIFDIKYSYFTVPLRKKTVE
                     PGIDVYDGECWVLFNPDRPPYWIPVTVEEAFRAAREFSAKETNEVAAAYNRQFLDQEW
                     AAIPEEDRQKPAYFGGGLARVSASPGYGGQDSIFPQIMKVNPAYPDRTLPHSAIQFMW
                     FRSVQNKQYMKKQLDECREYLKKERTSGCDLTRFELSFGMTDIRNLAEEIGR"
     gene            10975..12195
                     /locus_tag="PKK03_00855"
     CDS             10975..12195
                     /locus_tag="PKK03_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX82967.1"
                     /translation="MSSDWKKVFAIIFSGQLVSTLSSYVVGYAVIFWLSVETRSAEVL
                     AYATIASLLPQMVLGLFTGVFIDRWNRKRTMIFADLFIALCTLVLASLFWIAEPKVFY
                     VYLLLMIRSAGMAFHVPAMQASVPLLAPQDKLMKIAGINNMIHSVSTIASPALAALLI
                     SLFKLQWILLIDVAGAVIAVATLLMVHIPDPEKLEEVKPHLWRELLEGFKEIYSKPGL
                     LWLFILALTAMFFIMPVAALFPLMTLEHFSGTTYMMSIVEIAWGIGMLIGGAIIGYKR
                     LNNYKVVLINLTYLALGLTFVFSGILPPTGFAWFAGVTAIGGISMTLYSGSFTVIVQT
                     MVEPAALGRVFSLYGSITLLPSMIGLLATGFIADSIGIANAFIISGTGIALMGISAFM
                     IPPIRQMVRSEMKP"
     gene            complement(12320..13147)
                     /gene="pyrF"
                     /locus_tag="PKK03_00860"
     CDS             complement(12320..13147)
                     /gene="pyrF"
                     /locus_tag="PKK03_00860"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNX82968.1"
                     /translation="MTSRELFHHILNKKSFLCVGLDPEISKLPASLGGLEEPIFEFNR
                     RIIDATHRYTVAYKPNFAFYESYGARGMAALEKTVRYINKLDTNIFIIADAKRGDIGN
                     TSKMYARAVFGEMPFDAVTVAPYMGEDSVTPFLSYGGKWAIILALTSNRGADDFQYHP
                     GTGQRLFEKVLNVSQRWGTTDNLMYVVGATRAEMLADIRKIVPDHFLLVPGVGAQGGS
                     LEEVARYGINNQCGLLVNSSRGIIYADSSENFDAVAGEKAREMQEEMARCLAGRFKI"
     gene            complement(13159..14244)
                     /gene="prfA"
                     /locus_tag="PKK03_00865"
     CDS             complement(13159..14244)
                     /gene="prfA"
                     /locus_tag="PKK03_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNX82969.1"
                     /translation="MANNLILDRLIGVKKRFVEVGELLTSPDVMSDMKRYIKLNKEYK
                     ELKPVVEAYEKYKSAIDNLESARELLAAEKDEEMREMAKAEIDTLLQSLPDMEEDIRY
                     LLLPADPEDEKNAVMEIRAGTGGDEASIFAGDLFRMYSKYIEAQGWKVDMNYYTEGTA
                     GGYKEIVFNVIGDGVYGILKYESGVHRVQRVPQTETQGRIHTSAATVAVLPEAGEFDI
                     EIRNEDIRKDTYCSSGPGGQSVNTTYSAIRLTHIPTGVVVTCQDEKSQIKNLEKAMRE
                     LRTRLYNLEYQKYLDEISHKRKTMVSTGDRSAKIRTYNYPQGRVTDHRINTTLYNLPS
                     VLDGNIQELLDKLQMAENAERLKESNY"
     gene            complement(14373..15542)
                     /locus_tag="PKK03_00870"
     CDS             complement(14373..15542)
                     /locus_tag="PKK03_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNX82970.1"
                     /translation="MTDRSRYALRGVSSGKAEVHAAISRVDKGLFPRAFCKIIPDMLG
                     GDPAWCNIMHADGAGTKSSLAYIYWKETGDLSVWKGIAQDAVVMNLDDLLCVGVTENI
                     LLSSTIGRNKNLVPGEVIKALIEGTEKFLARMRDAGIGIWSTGGETADVGDLVRTVIV
                     DSTVTARMARADVIDNANIRAGDVIVGLASYGQTTYESEYNGGMGSNGLTSARHDVFG
                     KYLAGLYPESYDRGIPGELVYSGPMRLTDPTAVPGINAGRLVLSPTRTYAPVIRKVLD
                     VMRPAIHGMVHCSGGGQTKIMHFIDNLHVVKDNLFPVPPLFELIQKSSGTPWEEMYHV
                     FNMGHRMELYVNEADAAAIIDIASSFNLDARVVGRCEAAESRGLTISTDRGKFIY"
     gene            complement(15564..17540)
                     /locus_tag="PKK03_00875"
     CDS             complement(15564..17540)
                     /locus_tag="PKK03_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX82971.1"
                     /translation="MNRIIILIIAVTLLCPSVLQAQKQKTERAYAAYNAGSYFEAIDY
                     FKDVYSKTRDKQLKADMVFMVSECYRLINDPKNAELWYKRAVRSPFPKPEAQYWLAES
                     NKKLGRYPQAIEEFREYRRLVPGDPRTEQQIRSCELAIQWQSAPESYNVEELKEANTG
                     DSDFSPAYGRDDYGLVWFTSSRDDARGNKEHGATGQSFTDIFETRLDKKGKWSTPVPV
                     DFLNSEFEDGTPSFSADYSEVWFTRCEAGKRESKGCVIMYSTREGTRWSDPVKVELLA
                     DSLVAAHPSISPDGQTLYFVSDMPGGLGGKDIYRTTRTSPGSAWSRPENLGTDINTRG
                     NELFPFSRENGTLYFASDGHIGMGGLDIFKATPQGGESWSVENMRPPINSPADDFGIT
                     FEGDAEKGIFSSARKGRGDDDLFTFEMPPLLFSITGLVKDEKTGQPVQGATVQLIASD
                     GANMQTETGNAGDFRFSLRPEVDYIFLASKKGYLNGKEKETTRGQDKSRDFMATIQMT
                     PIDRPIELPNIFYDFGKWDLRPESMVSLDKLVETLVDNPTVTIELMSHTDSRDTEEFN
                     LDLSQKRAQSVVDYLIGKGIAADRLTARGYGETSPKVVDDEIMKDAPFLRLGASLTEQ
                     YINGLASDEQKEIAHQVNRRTEFRVLRTDYEPAK"
     gene            complement(17696..19885)
                     /locus_tag="PKK03_00880"
     CDS             complement(17696..19885)
                     /locus_tag="PKK03_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNX82972.1"
                     /translation="MAELRFSALKELFNRDPVEVSIPAAPVSTYFSENVFDKRKMERF
                     LSREAYRAVITAIDEGTQINRQIADQVATGMQAWAMEHGVTHFTHWFHPLTDATAEKH
                     DAFVGRGELGSVIEVFSGEVLVQQEPDASSFPSGGIRNTFEARGYTAWDVSSPAFIVG
                     NTLCIPTVFVSYRGDALDYKTPLLRSLAALDKAAVEVCHLFDKDVKKVIATLGWEQEY
                     FLIDEALYYARPDLMLADRTLMGHPSSKDQQLSDHYFGSIPDRVKAFMKEFEYEAYKL
                     GIPVKTRHNEVAPNQFECAPVFEEANLAVDHNQLIMDVMRRVARKHKFRVLFHEKPFA
                     EVNGSGKHNNWSMMTDAGVNLLSPGKNPKTNMQFLTFLVNVIKAVNDNQELIRASVTN
                     ENNSYRLGGHEAPPSIISVFLGSYLTEILDGIASKVKDKKMTPQEKTEIKLGIGKIPG
                     IFLDNTDRNRTSPFAFTGNRFEFRAVGSSANCSSAMIVLNAAVARQLKAFAVEVNAII
                     DRGGKRDEAIFQVLKNCIIDSKRIRFDGDGYSAEWHKEAIRRGLSDEPSVPKTLKTYE
                     NSSSRETLVGTGVLTARELESRVEVELEKFTKKLQIEARVLGDLAINHIVPTAVNYMT
                     SLVDNVKGLREIFDENEFIRLAGARKELIVTISDHISMIKKLVTEMIDQRRKANVIPD
                     LYKKALAYESKVKPYLEEIRTHIDKLELVVDNELWPLPKYRELLFTR"
     gene            complement(20009..22756)
                     /gene="fdhF"
                     /locus_tag="PKK03_00885"
     CDS             complement(20009..22756)
                     /gene="fdhF"
                     /locus_tag="PKK03_00885"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNX82973.1"
                     /translation="MEKIAYIDNRPFAIEPGETILQFVRRNSGHDVIPTLCQAENLDN
                     YGSCRICSVEVALKEGGPGKVMASCHTPVAPGYYVYPSTDRIRRLRKNILELVLSEYP
                     PERLIPEPGMLPTEFQTVVAAAGSPEVRYHRNVTHHEKDRSRPYVWSDLTECIKCYRC
                     VRACDELQAEHVLGIYGRGDDSRIIKGFDQSFRDSPCVSCGACVQTCPTNALHDRYSV
                     KTLKADRIVRTTCTYCGVGCNLDVKVADGQVRGIQAPLDSATNRGHTCVKGRYAFEFY
                     NHPERLRSPMVRKNGELKEVSWDEAYEYTAKRFREIKDKYGPDALAGISSARCTNEEN
                     YLMQKFFRIVIGTNNIDGCARVCHAPTAMGMQWAYGTGAATNSVNEIYKTGCILIIGA
                     NPSSAHPVTGARLRSVVETGTPLIVIDPLKIEIARLAKHHLQINPGTNVAVLNLFARY
                     LLTEGFIDSEFIAKRTEGWDEFRQYLERLDVDELERICGVDREKVRAAAIEYGSAKSA
                     MEFHGLGVTEHWQATKAITLISNIAMMTGNIGREGTGVNPLRGQNNVQGAADMGIQPH
                     QGAGYLDVTRPENIALYNAYYGAEHPAVPGYRIPEMFTAAARGDLKALWIMGEDILQT
                     DPNTCHVKSSLQGLDLLVVQELFMTETARIADVIFPASSFLEKEGTFTNGERRIQKVQ
                     RAVEPLEGTKPDGQIVVDMMNRMGYPQKGYSADVILKEIAGIVPFFKGVRWDELGNSG
                     KQWPVREDGTDTQILHTETFKRGKGRFHSWDYDVSPELNENASRFPYILTTGRLLEHY
                     NSGTMTRRTPNSELVTEDILFVHPYDAGAKGLVSGDYARIFSARGITTMKVKVTDVVK
                     PGVIYTTFHFPEAAINFLTSGIGDEFTLTPEYKVVAVDFEKSQYGIFTRAECRTIIKT
                     D"
     gene            complement(22759..24387)
                     /locus_tag="PKK03_00890"
     CDS             complement(22759..24387)
                     /locus_tag="PKK03_00890"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNX82974.1"
                     /translation="MSRNLRGLSARRDLTDSLYESIAGVSGDDDNARNENLKAIAGEF
                     MTGDAAILSSSTFYDFLRPAHSGKKVFMCDGTACLTSGRSEDVRRSLLESYREDEIGT
                     MTCLGHCHSNTAFLVNGNIRLCGSDADPSSMSIGSNADMPALLTPSGDTGNYWSLAEK
                     WTGRTDDALRELEASGLRGRGGAGFPFHVKVRSSRDAAADRKYVVCNGDEGDPGAFSD
                     KWLLEERPHAVLFGMMMTGIITGADTGVVYIRGEYPLAVRRMREAVTEIEKAGIATGT
                     EGNQITRFRFHVVEGSGAYICGEETSLLNSLEGLRPEVRTRPPFPAVYGLFGRPTVLS
                     NVETFANIHYILEEGGAAWAAMGTEKSPGPKLVSLDGAFKKPGLLEVKMGTPMRTVID
                     ELGGGTRYPVKAFQVGGPLGGLVPASRIDDLTLDFESFAREGFLLGHAGIVSVPADFP
                     VIKLLEHLFEFTRKESCGKCFPCRLGSARGYELLAGAIEKGEKIDRQLFDDLLETMQL
                     GSLCALGGGLPLPVRNALQYFAEELKDYFTVREA"
     gene            24577..26697
                     /locus_tag="PKK03_00895"
     CDS             24577..26697
                     /locus_tag="PKK03_00895"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNX82975.1"
                     /translation="MAILQSLREKAGPLLAIVIGFSLLLFVVSDFFGSNTSQRRKANK
                     YYQLATIDGETVSYQDFEARVQELVDIYKMSGSSEVNDEMMQSLREQVWQQMLSEKLM
                     GKTYRQVGLGVSPDEVEMLVFGDDPHPIVRQLFTDPGTGTFNESFLVNFLKATETDDA
                     TKRYWLFFEDQIINDRLNTKLVNLMSKGLYVTGKQSEFENSLNGNTVSFSYMVRNYAA
                     IPDSLVKVTPDEIRSYYDKHKDNFKRGATRDMEYITFEIAPSEADMKETETWAINEKE
                     NFATATDVIQYINLTADTRHTGFYLTTEDLPESLRPLAEAGDKSAVAGPYLENGSYKL
                     ARIIDIAERPDSVRAAHILLSPNPTRPLAQVRKEADSLIALVRSGIDFNVLAMANSDD
                     QGSAQVGGDLGWFSEGMMIIPFNNACFSNKKGDIVTAETTYGIHVIKILDQSRRVRKY
                     DIGIVDRQVIPSSMTTQRIYSEASQFAGTNDTYEKFNKAVAEGNLNKKLALNVTPDQK
                     ELPGLTQSRGLVMALFQNSKAGSIVLDNSSQAVFELPDQYVVAYCSRAQEEGVAPVED
                     VASDIRFILAKKKKAEVIVAEMKKLEAEGKNLNEIAAHYNTTVQDASGINFRSYSIPG
                     AGIEPALISAASASEAGKLSKPVEGNNGVYIFMVSDAAPVVAEELAMVKERMNSSYQI
                     RASYEAYQALRDKNEVKDMRYKFY"
     gene            complement(26775..28532)
                     /locus_tag="PKK03_00900"
     CDS             complement(26775..28532)
                     /locus_tag="PKK03_00900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HNX82976.1"
                     /translation="MKLLTSRELARAANLDIPGGVVIAKALMQIFRYNKLNRVYTNTY
                     DNDPAVFINSILDHLDIRYDIPDKDLENIPLTGPFITVSNHPFGGIDALILLKILARR
                     RSDVRVMANFLLQKIDPLKHLIVPADAEGESHEGMTSRKGMEEAMSHVREGHGLALFP
                     AGEVSTYQSESGLIADMEWQEQILREVRMAEVPVIPVYFHGTNSRWYHILGRIHPLLS
                     TAKLGSELFNKRHKIIRVRIGRSLSVSEQAGFGDIARFGRYLRARTYSLGSSLEAKPF
                     FSGFMPRRNRRQEPVAPPSDRATLAAEINNIRRTYQLFTVSNFVVLFAPTRAIPNLIR
                     EIGRMREVTFRAVGEGTNRATDLDEYDFYYHHLVIWDEAAGVLVGAYRIGKGREIMSQ
                     YGVKGFYINSLFRIRKSFEPVLAESLELGRSFIVQEYQKKAMPLFLLWKGLLSVLLQN
                     AGYRYLIGPVSISNEFSAFSKSLIVEFVRNNFFDREMAMHIRPRKKFSPQIDKRIDSE
                     IIISSAERDISKVEKVVSDVDNGYRIPVLLKKYLEVNARIIGFNVDPDFNNCLDGLIM
                     LDVYNLPAGFVSSLSREKE"
     gene            complement(28486..29958)
                     /locus_tag="PKK03_00905"
     CDS             complement(28486..29958)
                     /locus_tag="PKK03_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNX82977.1"
                     /translation="MAVFTPLRRWQPAFRPFSRQPAGKQLLALTERIVKGPLFGCRMC
                     GNCLLQETAFICHMACPKGIRNGPCGGVNNGYCYVDPARRCAWHLIYERSRRMHREHR
                     LLEVLPPVDWDRAGTETWGEVVRTARKTGIGKILVPSRRKGRTMGNMLEESVFKPIRQ
                     PDWWKGDTEYHPPVVSGPQSALEAELRSGRFVVTAEVTPPLTAATARLREKILTVMPY
                     VSAINFTDGSSAVPRMSSAACSAVAASLGADPVMQISARDNNRNNLQSLAIGANALGV
                     YNILCLSGDSPRVGPAPRSNMNIIDVDSVQMLWILRRMRDEGIYLDGREIKNRPKLFL
                     GAAAAPFALDPVMQAIRIRKKVNAGAQFLQTNLVFDPDRLDPWLEQLDMENLLDKVFI
                     LVGISPLKSLSIARYLNEKIPGVTVPERVMQRLADAGERFAEESIAITVETLLRLKSK
                     KGISGVHIMPLGWEDALPHIIEKAAHETTDLKGAGQGRQS"
     gene            complement(29966..31291)
                     /gene="xylA"
                     /locus_tag="PKK03_00910"
     CDS             complement(29966..31291)
                     /gene="xylA"
                     /locus_tag="PKK03_00910"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HNX82978.1"
                     /translation="MAKKSKSEIFPGIGKIQYEGRDSKNPLAFKWYDPEAKVGGKKME
                     DILRFAIAYWHSFCGDGTDTFGAKTRDFPYDGLEGDDKIKVKLDMAFEFFEKIGAPYY
                     CFHDVDLVGDDGSVFEIEKRLEKWVPVIKARQKETGMKLLWGTANVFGNPRYMNGAAT
                     NPDFNVLTNVAVQLKNAIDATIALGGQGYVFWGGREGYLTLHNTDMKRELEHLAMMLT
                     MARDYARKNGFKGKFYIEPKPMEPTKHQYDYDSATVIGFLRHYGLDKDFALNVEVNHA
                     TLAGHSFQHDLQTAADAGMLGSIDANRGDYQNGWDTDEFPTNINEITEAMLVIMMAGG
                     FKTGGINFDAHVRRNSTDPEDLFIAHINGMDTFARGLVIADRILKDSDYLKMRKARYA
                     SFDKGSGALFEKGKLSLGDLRELAKKVGEPKKISGKQELYEQLINMYII"
     gene            complement(31302..32789)
                     /locus_tag="PKK03_00915"
     CDS             complement(31302..32789)
                     /locus_tag="PKK03_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNX82979.1"
                     /translation="MLLLGIDLGSSSVKVSVIDGEKGRAISSASYPPSEMEISAPAPG
                     WAEQDPELWWKNFTLALAACLKPLGTKRKDIGAIGISYQMHGLVVVDKNRKVLRPSII
                     WCDSRAVETGERAFDRLGKDFCLHHLLNSPGNFTASKLAWVKENEPDIYRQIFKIMLP
                     GDYLAMRLTNEIKTSVSGLSEGIFWDFMRNEISEELLDHYGISPDLLADAVPSFSLQG
                     ELTAAVAADLGLTPGIPVSYRAGDQPNNALSLNVLKPGEVATTAGTSGVIYGVTDRTQ
                     FDPLSRINTFLHVNHTERLTRLGVLLCINGTGILNSWLKKNTGAGLNYPEMNDLAAAV
                     EPGSDGLIILPFGNGAERMFRNREIGASVHGLNFNIHRQGHLYRAAQEAIAYSFRFGL
                     EIMKETGINPTVIRAGESNMFLSKVFRDVLSSLTSTVITLYNTDGSVGAARGAGIGCG
                     FYRTDEEAFSGLEVTGSSEPDPRLAMIYDARYNEWLDILENHLYE"
     gene            complement(32802..33836)
                     /locus_tag="PKK03_00920"
     CDS             complement(32802..33836)
                     /locus_tag="PKK03_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNX82980.1"
                     /translation="MNDRHWDTLLKMIRGEKTQEPPVGFIIDSPWLPGWYGTTILEYF
                     CDPATWFEANLHAMKVFPDVMFIPGFWSEYGMCTEPSAFGTKMVWTENDFPHPVKLNC
                     TTDAVASLAKPCVKTDGLLPFVISRMAKYQDRVKQYGCHYRFASSRGPLNIASFLFGT
                     TDFMMALALTPDEAQKALTVITDFVIDWLELQYEKFPDIEGVLLLDDIVGFLGQPDFE
                     TFVLPHLKRIYSTFNPKVRFFHNDAAGLVTAPYLKEIGINLFNFSFNHSITEMRKLSG
                     DGVILLGNLPPRDVLAMGTPADVEAGVISMMREAGDTRGIIWSCGGGMPPDASTENIR
                     TFITAVRNFS"
     gene            complement(33867..36023)
                     /locus_tag="PKK03_00925"
     CDS             complement(33867..36023)
                     /locus_tag="PKK03_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HNX82981.1"
                     /translation="MHKILTVLLTILLLAPVAPAQADDGYRLWLGYKPVTDRARLKEY
                     QRRLENVMITGNSPVLRTAHDEFIAGLAELTGLKITEVNSLEGRGIVIAGTFSSSPFV
                     ASLLPDVVPGTTGEEGYIIRSIRQGRRNITVVASEGDRGVLYGLFHLLRIISTESPTG
                     NLDILERPATALRLLNHWDNLDGTVERGYAGGSIWNWHLLPGYISPRYTDYARANASI
                     GINGTVLTNVNANALVLTPQYLGKVAALADVMRPYGIKVYLTARFSAPQETGNLPTSD
                     PLDPAVAAWWKEKAAEIYSLIPDFGGFLVKANSEGQPGPQNYGRSHADGANMLADAVA
                     PYGGVVMWRAFVYDNNVPVDRAKQAYNEFVPLDGKFRDNVLVQVKNGPIDFQPREPFH
                     PLFGAMTSTPLMMEFQITQEYLGCATHLVYLAPLFSEVLDSDTYAAGPGSEVSKVIDG
                     SLHNHTLTGIAGVANIGNDRNWTGHLFGQANWYAFGRLAWNPELSPAGIASEWIRMTI
                     THDPDITGGIEEMMLGSREAAVNYMTPLGLHHIMAEGHHWGPGPWVDRGRPDWTSVYY
                     HRADTAGVGFDRTTSGSDAVSQYFPPLDSLFNDPQACPENLLLWFHHLPWYHVMKSGR
                     TLWDEMCLKYQEGVNTVNEYRTLWESLRGRIDDGQFEHVRQMLDIQADEARWWKDACL
                     LYFQTFSRMPFPDGVEQPQHSLEYYRVLKFHYVPGI"
     gene            complement(36029..37060)
                     /locus_tag="PKK03_00930"
     CDS             complement(36029..37060)
                     /locus_tag="PKK03_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNX82982.1"
                     /translation="MKKKGEVTIYDISRALNISASTVSRGLNNSPQVRKELRKKIMHT
                     AQEMGYQHNKFAANLRQKRTRTLGVIIPRIDSHFMSTVISGMEKVANNSGYQLLISQS
                     EESAALEEANIQALFNSRVDGLLVSLSYETKTKDAFANILRKDIPLVFFDRVFDCGNC
                     VSVVIDNYRAGYEATQHLVDQGCRKIVHVAGSLNRNVYNDRYRGYRQALMDNSMNFDD
                     KMLIITDLGDGSGVSIINHLLNNGTMPDGIFTANDNSAVSLICELKRKGFRVPDDIAM
                     VGFNDDPVSRIVEPNLTTVRYPGRAIGEVAASTMIRILEGTQYEKVNSIILSHELIVR
                     QSSLRKGQS"
     gene            complement(37296..38189)
                     /locus_tag="PKK03_00935"
     CDS             complement(37296..38189)
                     /locus_tag="PKK03_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNX82983.1"
                     /translation="MTKKYDITFVGHMCYDEIVPFGGTPRVAPGSAVLCGAMVAARVG
                     RKVAVVAKMARKDEEILQPMKDLGIDCYLIPSAETSYNRVVHFSANVDEREMTLIKSA
                     GIIDIKEIPELDSTCVHLAGISDTEFDMPLMKEMKARNFPSLSVDMQSFVRHINPVTK
                     NHEFSDVADKKEIAAMMDKLKLDVVEAKLLTGTDDLEKAAIIVESWGCPEVLITHSEG
                     VLARVKGETYYEKFSNNSVAGRTGRGDTTFAAYLSHRLDHEVRESLKFAAALVSIKME
                     TPGPFSGTLDDVYKRLKEKHS"
     gene            complement(38241..40496)
                     /locus_tag="PKK03_00940"
     CDS             complement(38241..40496)
                     /locus_tag="PKK03_00940"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HNX82984.1"
                     /translation="MLTQKLPGETELPDDIPAVKNYYPAPVEKENIALTIATPSLEIA
                     YKFENVKVIRRLISPLLSGGEQTLMPLGVEEYVVENVTGYDQQVTIVIPRPSLVNLQE
                     KELKPTDQDSVFVATAAVKEQKHEEFRLEGIRGIIMGSNESQNRMAIAVTDVPGVAVD
                     TQPYFCLNKYSGDLLLSSDGSFYEKRQPVIRSDYGAAVSLTFSLGPFESKTVPFAVAL
                     DFPVQDYIDGTTFERKYVKSFSNGETRILDLVKIALTSYPQWWKRTVTIQKRIFDLIS
                     STSSYKEDREGALRLTRLMLNELHFPLSNAIVWVDDEKGERARFLECFDYAYIDPSDV
                     DWYSMVLLMLFPKVEKDLCQAFINSILAVDPTPRWYHHHASFVQAQMHFNEHREEYEG
                     VSLRQIRDDFKTKGSVAHDVGAMPKGHPLRNVSDYAWYNNNYWVDLYPKLMMRVLRNV
                     KFTGDMEFLRSNWETMKFGLDSLLKLDFDNDGIPEGHPDDVKNTFDNLVLFGADAYDA
                     TQFLGGCQALIKMAQMLGEKGDEEYIRGKYDKAWNSFEKLWREDVNKKGEKLEYYVTC
                     FDPETGNTNTDVWTNQLDALWYLTAIGEPPAIPEERVKKILKAVYDTNRSYMGWAMCK
                     TKDGEPVESEQGQDVYTTTNYVFAQLLDYYGMVEESKEVYKHMDRVVFKYANTLTTPD
                     NLRAELELEAGEKEPCPHYIVAAYPRPGAVWSHLVMNHIKDLQAKNKTMTIGPEDLMP
                     FYPWLLSDSEE"
     gene            complement(40937..42772)
                     /locus_tag="PKK03_00945"
     CDS             complement(40937..42772)
                     /locus_tag="PKK03_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNX82985.1"
                     /translation="MSKERDDFVAAKIAKMSLLEKCGQLLTFTWRGAIPTPSGIEQIT
                     KLHAGGICLEPYALETCKNLYWGRSQVDPNFKKPDDYFDIAHTWFNSRAFGISVTPEE
                     LTEALNNMQKIAMNRPSGIPLHVTIDMEGDFKNDYTAGGVLQFIPPMGITAIGDVDLA
                     YKIANLMGRQMAAIGVTQFYHPVCDVNINPLNPEIGVRSFGDNVSVISKFIDATVRGY
                     EDAGITATIKHFAGRGDSATDAHDVLDVCRGDKKRMQDVELAAFQAGVDAGASALMTA
                     HTIFPAYDEEYPATLSKKILTDLLRGEMGFDGIIVSDAIGMAAILKKWPLPLACAMAI
                     KAGVDTILLKADDESRSQCLFGIKSAVENGTLPEERVNDAVRRLLCRKYDQGLFEKAG
                     KMDPKETTKIVRSKENRDFSREVAHKALLVVRDDKKLLPLSKDKKILVIEQTIPYEFL
                     GKDLYSHAHMFCEQMVKHSTNLILDDAPFHAEEEDIEEALRLAKEADLVVMTNYYARI
                     EKRGNNTHLARALKAAGHTVVVVTNFPYRKGTTTVADAVVCNFSGTPDSIRASADLLF
                     GAIKPYPTTKMPIDLSAEQNLPEEPDPAPRKSQEKKPGNVWGGKC"
     gene            complement(42844..43965)
                     /locus_tag="PKK03_00950"
     CDS             complement(42844..43965)
                     /locus_tag="PKK03_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNX82986.1"
                     /translation="MKIKTITAIIAIIFMSNIYAMAQKTIAKATKGKFLFGVAVNMQQ
                     VNGNNPIESELIAREFNSIVAENCMKPQPIHPQEDKYNWEDADKFVAFGEKNKQAVIG
                     HCLMWHSQIGRWFFVDEEGKDVSPEVLKERMRQHIYTVVGRYKGRVKGWDVVNEAFED
                     DGSYRNSKFYRILGKDFIKYAFQFAHEADPDTELYYNDYNVETPAKCDAIVELVKELK
                     AAGCRIDAVGSQGHMHMDDPTLEATETSLMKLKAAGVKVHITEWDISILPSPYSGANI
                     STRFTYSKEMDPYREGVPDSVQQEWNKRVLDMFELFLKHNDVVDRVTLWALNDSTSWL
                     NNFPIRGRKDYPVLFDRNNQPKSVVADMIMMAKKYKKKR"
     gene            complement(43994..45460)
                     /locus_tag="PKK03_00955"
     CDS             complement(43994..45460)
                     /locus_tag="PKK03_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX82987.1"
                     /translation="MSLTSQKISVFEKIGYSLGDFAANLVFQTLITYLAYFYTDIYGL
                     KSTDASIVMLIVGLVAAFGFNPLIGILADRTNSRWGKFRPWILFTAIPLGVIAILAFS
                     TPDFSYKGKLIYATTTYTLLLLMYASSNLPYAALSGVITGDMGERNSISSYRFVAVMF
                     AQFFVQVFMLPIIEYVGHGDKAAGIESVMTWMSVIGTVMLIISFLSTRERIVPDVEQQ
                     SSVKDDLSDLSKNRPWLIMLVLTILVFVTLAMKGGAYVYYFNNYVDEAALTRFIHPII
                     TGLSGIGINFFGSDPVSAGFGLFNAGGIIFMIVGISLSKKLADKYGKRDVYKYGLLAA
                     AIFVLIFIFFKPESVILMFTSQILHGFTYGITIPILWAMIADVADYSEWKTNRRATAI
                     IFSAMMVGLKAGLSIGGALVAWILGLYGYIPIEGTVAGAEIIQPETVATGAKMLVSVY
                     PSIPFLIGVGLLFFYVIDKKMEVKIESELHARRGQVVA"
     gene            45887..47272
                     /locus_tag="PKK03_00960"
     CDS             45887..47272
                     /locus_tag="PKK03_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82988.1"
                     /translation="MKRLTVFAISAGIILLGQFCGDPEGPPDPVIEPEINKAVIDPAV
                     SYQEMIGFGGSLTWYSERVTSSPQKEQICKLLFEDLGTDIIRFKNNYYPAGYPGVKSP
                     ATMENSSIKTLWNVTGDLYAMAKQFNPGIQILVSSWTPPSALKSNNNLREGTLKRNNG
                     VFMYEAFGDYWNDLLDNLQFNPDYISIQNEPGWITPDWETCEWRPAETAEFPGYNAAF
                     DAVYNKISTRSNPPMMIGPEAENIGYSGKLGGNTFAVFSDPIKNKESLAAYAYHTYNF
                     PSSALIAQTKSDLNMIRDTYGNKPCIMTEYSNFTWLNTAWFIIQNINEANAAGYIYWL
                     MAWADSNNDSMIKLSPSGAYTLTPFYYVMKHFSKEVDKGYVRIKVLSPLMPMCGFIDP
                     TGKKITVVAVNPNLESAIYDFDVTGKIVKSVRAMQTDAVVSYKTIQPSGPGQRFVLKP
                     QSVTTVVIELQ"
     gene            47294..50278
                     /locus_tag="PKK03_00965"
     CDS             47294..50278
                     /locus_tag="PKK03_00965"
                     /inference="COORDINATES: protein motif:HMM:NF027309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HNX82989.1"
                     /translation="MKKAVAARLGDLQAIFKSVLPSLFLICLPGFSQGETTVIISTEK
                     SDGSFVLASGGRCGSLFISPDEWPGVTRAFTDLQNDLGHITGVIPDLITASKPPRAEF
                     MIIAGTIGKSTTIDRLIKRKKIDADAINGKWESFVIQTVEKPFPGVKSALVIAGSDKR
                     GTIYGIYEISRQAGMSPWHWWADVPVRKSSELYVNPGRHEWGEPSVRYRGIFLNDEYP
                     ALTRWVAYKYGEVTPSANPPIPPGIANYGSEFYSRIFEVLLRLKANYLWPAMWNNAFN
                     EDDLRNAALADEYGIVMGTSHQEPMIRAQKEWDRRYQKTLGSWSWTHHEDTLLKFWRD
                     GIRRNRDFESIITIGLRGADDTEMGPGGPAANIARLEKIVKVQREMIAEEIDNDVTKV
                     PQSWCLYKEVQDYYYEGMRVPDDVTLLWAEDNWGNVRRLPTADERKRPGGAGVYYHFD
                     YHGGPRSYQWINTSPIPKIWDQMSLAKQYGADRIWIVNMGHFRGYEIPMEYFLDLAWD
                     AGSLDSDDMSAWTQQWVEGIFGPEHAGEISEIIREYTRFNGRRKPELLSPFTYNLVNY
                     AEADRIVEEYNILAERAEKIGHLLPAEMKDPYFHLVLFPVRACALVNELYVTAGKNAL
                     YASQGRANAGKMADRSEQLFAADTALMGYYNRVYAGGRWKHFMDQSHLGYIAWNDPPE
                     NSLRHIKLERPAPSLGAIPAVAVEGSAEAWPGPVTPPLLPPFDVFNKQSRYFEVFNRG
                     SHPFEYTASADHPWIILSSSHGIVTEQERVTVSIDWPAVPHGHHNGTVTLTAEGSEMK
                     IAITAFRPEDPDPETVEGFVEADGYVSMEAASYTGRHDTGDRRWEWIEDYGRTHSGMR
                     TSALTDAPPAVPGKDSPVLEYKIYFFSAGEFETVLHMAPSLNFLPERNFRIGLSVDHS
                     DPQLITVVPAGFNAENFNREWEEIVSNSTRYVTGKIKVAGPGSHTLKVWMIDPGMVLE
                     KIVVNTGGLRPSYLGPPESHRN"
     gene            50447..>51320
                     /locus_tag="PKK03_00970"
     CDS             50447..>51320
                     /locus_tag="PKK03_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX82990.1"
                     /translation="MKTKSYLFLATLFVILLTFPLTAQVRLPRLISDGMVLQRGTELK
                     IWGWAAPGENVNVEFAGKQYSTITDDSGNWMIMLPPMEAGGPYIMTLTASNKLTVSDI
                     LIGDVWLCSGQSNMELPVRRVRPLYEAEIAAAENNLIRSFTVPKTFVFTGPESDLPGG
                     KWTAANPETVLDFSAAAWFFAREINQTYRVPVGILLTAFGGSPAEAWVSEESLKAFPE
                     YYDELRKLNEDSYINNIEKEDRQRIADWYSNLQKTDLAYKTEDLRWNDIDLNTDEWSS
                     YTVPGYFSATPLKGI"
BASE COUNT        12422 a        13215 c        12806 g        12877 t
ORIGIN      
        1 aattagagca attatacgat atttctgatt tgtctgcaaa tttaagataa tttttagtca
       61 atacctgcaa ccactgtatg aaaaaatcag aaattccgga ggttgatccc ctgagcgaca
      121 ggttcggtga agatcatttc ctttacttta aggtagtcat catggcatgc aacaaagtcg
      181 atatttgaaa catcaagtac caggtagcgg ttctccttat tctgcctgaa gaaggtgaag
      241 tagctctcct gtattcccga gagataatcg gcagttattg acttttcgta ctccctgccc
      301 ctcattgcaa tgtttttcag cagcctctca gggggtacat gcaggtaaac ataaaggtca
      361 ggcttgggaa gggagctata gattatatag aatatctggc ggtaaaggct gaactcatcc
      421 tgttccaggg ttttggatga gaagacgagc gacttcatga agtaatagtc agccactgtg
      481 aagcttttaa aaagatccgg ttccaccagc tcttccttga gctgcctgta gcggtcggcc
      541 aggaaggaga gttcgagggg gaaggagtac ctagacgggt cattgtagaa tttggggagg
      601 aaaggattat cggcaaaccg ttcaaggatc agtttggcat tgtagtccct tgctatcatt
      661 gatgccaggg tggtctttcc cgcgccgata tttccctcaa ttacaatata gttgtacttt
      721 attttctcca tccctgttgc cacattgcac cggaattcaa aattaacatt taggcttcac
      781 aatgcaaata aatcgaagaa gcgcgtttcc gcgactccgt tgtaacggga aaaagagagg
      841 ctgccccgtt tgagacagcc tccgcctgaa taacgatcct ggatgaataa ccggatttct
      901 accggttgtt gtcgcgacgg cggtcatgcg agcgatgctc acggtttccg cggtcaccgc
      961 gatcactgcg gtttccgtca cgtgatccct gataatccct tctttccgta tgttcacgtt
     1021 tgggcggctc aacccagcct tccggcttcg ggatgagggg cttgcgcgag agcctgagct
     1081 tgccggtctt ctggtcaacc tcaatgagct gcacctcaac aatgtcaccc tccttgaaga
     1141 gctcttcaac agtgcctacc ttcttccagt ccatctctga gatgtgcagg agtccgtcct
     1201 tgttggggag aatctcaaca aacgcaccga actgtgtgat ggtcttcacc ttgcctgtat
     1261 agacctttcc tacctcaggt acggcgcata tcgcgttgat acggtcaagg gccgccctga
     1321 tgacttcgta gttatcgcca aatacatcaa ccattccctg gccgttgatc tcttcgataa
     1381 ctatcgttgc gccggtggtg gcctgtatat cctggatgat ttttccgccg gggcctatca
     1441 cggcaccaat catctccttg ggtatgtaaa tcttctctat tctcggtgca tgaggcttga
     1501 ggtcgggcct gggctcggca atggtctgtt ccatgatgcc gaggatgtgc atcctgccgc
     1561 ggcgtgcctg ttcgagcgcc tgcttaagaa cctcgaacga gagaccgtca accttgatgt
     1621 ccatctgtgt ggcggttatt ccgtcacggg tgcctgtgac cttgaagtcc atatcgccga
     1681 gatgatcttc atcaccgagt atgtctgaaa gaattgcgaa tttcttcccg tccttgtcag
     1741 ttatcagccc catagcgata ccagaaaccg gcttggcgat ttttatgcct gcatccatga
     1801 gtgccagtgt cccggcacag acggttgcca ttgatgatga gccgtttgat tcgaggatat
     1861 ctgataccac gcgtattgcg tacggattgg tctcctcggg gggcatcatg ttcttaagag
     1921 ccctgtgtgc caggtttccg tgacctatct ccctgcggct ggtaccgcgt gctgccttgg
     1981 cttcgccggt ggcgaagggg ggaaagttat agtgaagagt gaatttctcg gaaccctgct
     2041 tgaggacctc gtcaatcatc ttctcgtcaa gcttggtgcc aagggtgaca gtggtgagcg
     2101 actgtgtctc tccgcgggtg aagattgccg agccatgggc tgcaggaagc ggatcaatct
     2161 cgcaccatat ggggcggatc tcatcaagct tgcggccgtc aagacgcctg tgctcatcaa
     2221 gcacaagacg acgggcagct tccttctcca catcatggaa gtacctgttc accagcatct
     2281 catctgcagg tgcatcctca gggaactgtg aaaggaactc agcctttatt gcgtcaaagc
     2341 cgtcagtgcg ggtcttcttg tcagatccgc tggcagccag cttgtaacac ctgtcatagg
     2401 tctcatccca tattttcttg cggagctcct catcattctt ctcatgacag tactctctct
     2461 tcacggactt gccgaccaat tcggagagct ctttctgagc aacgcactgc ggtttgattg
     2521 cctcatgggc aatgcggagt gcctcgagca tgacatcttc tgacacctcc ttcatctcgc
     2581 cctcaaccat catgatattc tcatatgttg ctccgacaat gatgtcaagg tctgcagttt
     2641 ccacctgctc aaatgtggga ttgacgatga actcgccgtt aacccttgaa acccttacct
     2701 ctgatatcgg gccatggaaa ggtatgtcag acactgccag tgccgcagat gcggcgagtc
     2761 cggcaagcgc atcaggcatg atatccttct cggccgagat gaggttaatg gttacaaaca
     2821 cttccgcatg atagtcgtcg gggaacagcg gacgcagcgc cctgtcaacc agtcgtgaga
     2881 tcagaatctc attgtctccg ggccttgcct ctctcttgag gaagcctccg ggaaagcggc
     2941 ccgatgccgc atacttttcc ttgtactcaa cggacagcgg catgaagtct gtgtcttcct
     3001 tagcatcctt ggctgcaacg acggttgcca gaagcatggt cttacccatc cttacaacca
     3061 cagcgccgtc agcctgcttg gccagcttgc ccgtttcaat tactattgac cggccatcgc
     3121 caaggtcaat acttttttcg attaacttat acatctgaat tactgattat tacgggataa
     3181 atgattattc aggctcagac ctctccggga attaaaaaag gcaatctgaa attgcctttt
     3241 ttccttactt gcggaggttc agtgccttga tgatctcacg atacctgctg atatccttga
     3301 cttttagata atcgagcaaa tgccttcttt ttccgaccag agtggtcaac gccctctgtg
     3361 tactgaaatc cttcttgttc ttcttcagat gctcggtgag atgcgaaatc ctgtaggaga
     3421 aaagagcaat ctgaccctca gcactaccag tgtccaactc tgatgatccg aatgtcttga
     3481 aaaattcctg tttcttctcc gtggttaaat acatatcctt atccaataga ttgttaataa
     3541 tttctccaat tttggaacgc aaagatacaa ctctattttg taataattca aacaatttga
     3601 ataatttttt tgtatttaac tccggacagc ctcaccagtg tgcgggggag tgcagcactc
     3661 acgggcttct gtataaaaga cgcaaattta atgccgggag ttgcgcgtgc taaaaaatat
     3721 attttgttgt ggccgaagcg tgtcagggct ccaggaacag cgagtctgac ctcctgatct
     3781 tctggcccat agcgatgagc gatgacccga tggtgcagtc aagacacctg tgatacctgc
     3841 atctcaggtt cctcagctcc agcagagcct gtgaagcgaa ggccgactcc ggcttcagtc
     3901 ccgcccgggc gaagtcggtc accacactgt tctcttccgg gggcagagag tcaaggatct
     3961 ccacagccct gtcacaccac tcctgctgtt gcctgacctt gccgtagacg aataacaacg
     4021 gtgcaatggc gttgatgatg agcaggtcta cggactgttt gccggcgcgg cccgccaccg
     4081 gaggcgcctc gcgtccgaac tggtagtgac tgttccagta agatgaggcg ctgacgctca
     4141 gcagtgcacg gaggctctcc cggtcactgc agccaagcac cctcgagaag agcccgtcac
     4201 tgtggctgag cagtgccgcc agttgggaga gcctgacagt ggggaaattg gcgggccgca
     4261 gcctgtgaaa cttccagagc catccgtcaa cgggctgaag tgagtacttg gcgcgaagca
     4321 cccggtactc cctcgacaac aggagataat aaccgtcgct caccgcctcc ctgaaaagag
     4381 ccccgtcaag caaccccgca gtgccataca gcaaggcctc gacctgcatg agctggtcag
     4441 cgtgctttct tatgatttta agaggaaggc gggctgccag tatctcaaag ggatcagtgt
     4501 ttaccctgaa accgaagtag cgggtgacca gccggtaaag agtctcttcc cagtcattgc
     4561 cggtctttgc cagaatctcc ctgatctcgc catgtttcct ttcgagccgt tcgacggcaa
     4621 cggaccacag ccagtgtttg atcctgaaac cgtcagaaaa tcctatcagt ccgctgcatg
     4681 gcaggggcga aggattattc atgaaatcga ggtagttttc ccacaaaacg ggatcgaaaa
     4741 cggggcgggc tgttatcagc cttcggccgg aagccgtata gacgtctgca tcctcattat
     4801 atacaaggtg cagaatgacg ttgtcataag cgtgatcagt gtgatgcccg tgccggtacc
     4861 aatcagatgc atttacatgg atctcggtgt tcccggccca ttcggtaccg ccgatattca
     4921 gcctcgtgtt gaaaaagtca ggaccggaat cacggttgta ctctcccggg gttattacct
     4981 caatggggcc ggcttccctg tcgcacaggc tcccgccctc aaagaacagg tttttccaca
     5041 gccagtggag gaactcttct ttcatgatag gtaatcttaa gaaatgcagc ctatcaaatt
     5101 taagaacaat gttccaaaag cgcaaaaaaa agctgcatta cgggcagcac ctgaattatt
     5161 tgacgaagca gtttaaaaat gatgtgaatg aaataacaac aacagcaggt tttgctataa
     5221 ttttactatt ccttaaaccc caaaccacaa ttattataat tccataccag tatgaagaca
     5281 cttactaaag ccctggctgt gctggttcta ttcagcatgg ccatcaatgc agaagcccag
     5341 attaaagtag acctgaagaa gaaactcgag cagcaggtca accggcgtct taaccagaaa
     5401 gctgaccagg ccattgacaa ggctcttgat acagttgagg acagtatcag gggtgatgta
     5461 aagaaagacg gcaatgcagg cggcaacgca accggtcaga acagcggcaa tgcccagccg
     5521 cagggcaagt cgggccagaa cgcggatgca cagtccggcg gccagcagga gcctgccttc
     5581 cagtcatatt caaagtatga tttcattccc ggagagaaag taatcttcta tgatgatttc
     5641 agtcaggatg ccgtgggcga tttcccggca ttgtggaaca ccaacggctc tgccgaggtg
     5701 gtgaccacaa atctctttcc ggggaaatgg atgaggttcg tcatgaacca gtgcgtctgg
     5761 acggatgaac ttctcaaact gcctgagaat tatacaatag aatatgatgt gattccccag
     5821 ggcgggcttg aaggcgaggg gatgtcaggg tggcacatga aactcatgca ggctaagaat
     5881 gcgaaggcat gggatggcgg gactcaaccc ggaaacggcg gtttcgaatt caggattgag
     5941 tattttggca ggccaagcta ccacacatgg ctttacgggc aggagtgctc ggtttcaaac
     6001 ctgagcggtt atgttgaagg tgaacagtac aagcagaaga tcaacaagct gtaccatata
     6061 gcaatttgga ttcagaagac aaggatcagg gtttaccagg atcagaacaa gattattgac
     6121 cttccgaggg cattccccgc cgggtgcgtg aagcctgaca ggatccgctt tgaatatggc
     6181 gctgcgatga tttccaacat ccgcatcgcc gtaggcgccc ctgacatgcg caacaagctt
     6241 atcaccgaag gcaaactggt cacttacgga atttacttcg acgtcaacaa ggacgttgtc
     6301 aagcccgaat catacggaac cctgaaggag attgcaacag tactgcagga aaacccggat
     6361 gttaaggtaa agattgtggg ccatactgac tcagacggcg ccgatgcctc caatctcgac
     6421 ctttcaaaga ggcgcggcgc ctcggtgaag aatgagctgg tgaagaactt cggcatagag
     6481 gcctcccgcc tggagtctga cggcatgggt gagagccagc cggtggcgcc gaatgacacc
     6541 ccggccaaca aggcactcaa cagaagggtt gaactgatca aactgtagaa ataatttccc
     6601 tgaactttat cattccggac aaataattaa taactgcaag cagatgaaac ggatactgtt
     6661 gatattagtg gcagccggta ttgttcttac cggagccgca cagacgcgga cacaggaata
     6721 tatgaagaag ataccagccc tcccgaagga ttcatgcaat gcgacaaggt catcggttga
     6781 aggctttgtc agccaggtct cggcactaag ggacgagctc gtcaccgaga tcgaagggat
     6841 aagggaagcg gttgactcgc acatggaatc aaatgccgat gtggcagagg caaatgccat
     6901 gaagcagatg gcacagatgt acggcatgtc acaggctgac atcaacaagt tgaaaaatgc
     6961 aaaggacatg tcagacgctg acaaacaggc ccttgcaaac aagatggtga tgcagcaggc
     7021 caacatgagt atggatgagg caaagaacct ctcgaaaatg agcgaggcag gaaggaaggc
     7081 atatgccgag gcgtatgcgg ttgaagcaat ggcaactgca cagaatgatc cgaaccagga
     7141 agccaaaagc aacaatgcaa agaacatgta ccagagcggt gttgcccagc aggctgcgat
     7201 ctcaaaaatc aatgagataa ccacccggat cgcagccctc tacagcccca ttgaaaaaga
     7261 ccctgaaagg cagaagatgc ttgaccggat gaaagcctgg aacaaccagc tgacttccat
     7321 gatgggaata gtcagtgacg gtgaggctcg cataatggac tcactcagcc tgagaataca
     7381 gaatgagcag atagcttact gtaacaaata cactccccaa tacagggctg ctctgcgaaa
     7441 acatcttcag acagtgaagg catcgatgcc tgactacaag aaattcggag acataaacgc
     7501 ggagacaacc aaaatccaga ccggaattga catgccggaa gaggggaaag aacttgcctc
     7561 cctggaagca atagccgatt acctcagggc attggaggag gtttacaggt acaagctgta
     7621 ctattctgat gaatactgat tatccttctg acagaggttc cgtactgtac atattaatta
     7681 atatttgatt gcatgagaca catacttgtg ttactgatgc tgtctgtatc ccttctgatg
     7741 agcggacaga caggccagtc tacggatcag atcaggcagc ggatggctga gatcaggaga
     7801 accaccaact gggatgaccc tgccgcagcg gcgaaagcca atgccgagat aaaaaaactg
     7861 gcagcacaga tgggcggtgg acaaccccct gtgagtttcg gcagcggaca ggccggaaag
     7921 gcggaaccag ccacgccgga aacaatcgcc gtaaagtcgt ccgatgtcac aaccggaaat
     7981 atagcagcaa tcgcacagcg gttttactcc aggtcataca aaacactgga tgcggccggt
     8041 aaaacacagt tcgaccagca tttcagggaa gcggctgctg atgacttcac attccggtca
     8101 gtaaggaagc tctccggcat cggcggagtg ctcatcagca tgggtgatga ccataacctc
     8161 gcatgcgtgt atcttgcagc agccgtcaaa gccatgcctg atgacacact gagtgtgaat
     8221 aacttcgggg catatctcag aatcattgac tcagtaaaag cttccattcc ggtacttctg
     8281 tatgcagaca aactgtacgg agaaagtccg attgtgctga cacagcttgg ctgcagctac
     8341 ctggaactcg gtcatgagaa ggaagccgag agctatctta aggaagccct ggagtataat
     8401 cccggttttg gtcaggccca tacggctctc tgtgagctgt atatcaggca gggcaggctg
     8461 gaggatgcgc ttgttgagtt gtttgcagga gtaaaaggga tgggggcctc ctatgcaaga
     8521 gcatcaggca actacgccta cctgcagcag caggctgaaa aagcaggtga aaacggcaac
     8581 cagagcgcca aggaagcatt ctgggacgag accaggaacc agatcagtcc cgatgatgcc
     8641 ctcgcacccc tggtgccatc tgttgatcgc cttaaaatgc catcctttcc aacttgccag
     8701 aagataaccg actggatgga gggaggagga tacgggactt ccgtacagac ttacaacagg
     8761 atgcacagtt tgctcatgaa gttttccgaa gagttacttc aggtccataa ggaattgccg
     8821 gagttgcctc ccaatgccgt acttcgtgat tatcccaatg aaagattcgc ccttgactgc
     8881 ataacagagt atttcttcag ggagtcggag gaagaggcac atgacttcaa cgatgcatat
     8941 gatgcaataa tggacgaagt caatgctgag gcagaagcct atcttcagaa caaggagacg
     9001 tacacaaagg agtacgtgcg ttgcgctgag gggtgcggcg gcgacggcta ctgtattgag
     9061 gagtgtcacc gggtatattg cacgaaggaa tgtcctgcag ccaatatcta taataaaagt
     9121 ctgcaggggc actgggaaga ttacctgtac cagttcaatg aaactgtcga caggcagaag
     9181 gagatactga atgacctgta tgaattttca gcccggtggt tctcgaagat tgaaagtcct
     9241 tactggagta agatttatgc ctatgaaata caacgggttg ccctttcggc gatcatgaat
     9301 gtctactcgg cacacgccag acccttccag tggcctgcgc ataatgactg cggcaccgac
     9361 tgctccgcgt atgccaatcc tgcaccgccc cctccggaag atgcggagga aaaggaaccc
     9421 aaagggaatg aatgccctga agataaaaag ctcaatatcg gcctggggat atgcagtatc
     9481 gcacttgatt gtgagagtgc ggaattcggt tgttcggcag gtgccgctgt ttcaataaaa
     9541 agaaatttca aaaataagag cacctccact tttcttggtg tcggcgcaga ggcagggctg
     9601 ggatttgcac acgctgaggc aaatgccggg gttactatga ccaggcatga caacggagac
     9661 ctggatgtgg gcgtaaaggc ggagctaacc ggaactgtcg gcgtgggccc cggatcaaca
     9721 ggcaaaaact atgaaatgaa tgtaaccgtg atggaaggtc tgaaaactga aaccaaggac
     9781 gtgataggca ttggttttta atcattaaat attcccggac tcagggattt acacaaacga
     9841 caagcgctat aatgaaaaag acacttttct gcatgctcat gcttgcagcg gctttgagtt
     9901 cccccgcaca ggataatgcc tcgcttgctg acaggccggg caccttcgag atactcagcc
     9961 gcactgacta cgcctggcac gaatgcggat tcacacaggc tgacatgacc gccaacctcg
    10021 aaagaatcaa ggatgtaatt gaggtggtca ggcagaatcc tgttctcagt gatatcaaag
    10081 gctttgccgc ccgggcaagg ctccatacca tgtcaatgac ctgtaaacgg gaggtgtggt
    10141 atggtgtgcc ggtaagagtc gcctttgaaa tatgtacttt cttttacaat aaggaaggca
    10201 aggtggtcta caatacaatg gagcctccgt cgtggtcact ctgcaccaac gactttattc
    10261 cgggctggtc tgatattttc gacatcaaat attcctactt cactgttccg ttgaggaaga
    10321 aaaccgtgga gcccggcata gatgtctatg acggagaatg ctgggtcctc tttaaccctg
    10381 accgtcctcc ctactggatc ccggtaactg tggaagaggc tttcagggca gcaagagagt
    10441 tctccgcgaa agagacaaat gaggtcgctg cagcatacaa caggcagttt cttgaccagg
    10501 aatgggcagc aataccggag gaggaccggc agaaaccggc atatttcggc ggcgggctgg
    10561 cacgggtatc agcttcgccc ggctatggcg ggcaggacag catattcccg cagatcatga
    10621 aggtgaatcc tgcttaccct gaccggaccc ttccccattc ggcaatacag ttcatgtggt
    10681 tcaggtcggt tcagaacaag cagtacatga agaaacaact cgatgaatgc agggaatatc
    10741 tcaaaaagga acggacaagc ggatgtgacc tgacacgctt tgaactgtca ttcgggatga
    10801 ctgacatccg gaaccttgcg gaggaaatag gcagatgagg taattgtcgg ttctgcaggt
    10861 acgaaatctt gatctcagca aatcaggatc agaaatttct caagtcagaa atattgatca
    10921 actttgcatc ccggaccatt gttttattag tcacaattat tcacgggggt caatatgtcg
    10981 tctgactgga agaaagtttt cgcaataatt ttttccgggc aactggtctc aacgctgagc
    11041 agctatgtgg tgggttatgc cgttatcttc tggctcagtg ttgaaacccg ttctgccgag
    11101 gtgctggcct atgccaccat cgcttccctc ctgcctcaga tggttctcgg cctgttcacc
    11161 ggcgtgttta tcgaccggtg gaaccgcaag cggacaatga tctttgccga cctgttcatt
    11221 gcactctgca cgctggtatt ggcatcactc ttctggattg ctgagccgaa agttttctac
    11281 gtttatctcc ttctgatgat tcgttcggca gggatggctt tccatgtccc ggcaatgcag
    11341 gcttctgtac cactgctggc gccgcaggac aagctgatga agatagccgg gataaataat
    11401 atgattcact ccgtaagcac catagccagt cctgccctgg cggcgctgct catcagcctg
    11461 ttcaagctgc agtggatact gctgatcgat gtggcaggag ccgtcattgc cgtggcaacc
    11521 ctgctgatgg tccatattcc cgatcctgaa aagcttgagg aggttaaacc gcatctctgg
    11581 agggaactgc ttgagggatt taaggagatt tacagcaagc cggggttact gtggctgttc
    11641 atcctggcgc ttactgccat gttcttcatc atgccggtgg cagccctctt tccccttatg
    11701 accctggaac acttcagcgg aacgacgtac atgatgagca tagttgagat agcatgggga
    11761 ataggaatgc ttatcggcgg agccattatc ggatacaagc ggctgaacaa ctacaaggtg
    11821 gtgcttataa acctgaccta ccttgccctt gggctcacct ttgtgttttc gggaatcctg
    11881 ccgccgacag gtttcgcatg gttcgccgga gtgactgcaa taggtggcat ttccatgact
    11941 ctgtattcag gttcattcac agtaatagtg cagactatgg ttgaaccggc agcgctgggg
    12001 agggtatttt cgctttacgg gagcataacc cttcttcctt caatgatagg tcttctggcc
    12061 accggattca tagctgattc aatcggtatc gcaaacgcgt tcattatctc gggcaccggc
    12121 attgccctga tggggatctc tgcattcatg attccgccga tacggcagat ggtaaggtca
    12181 gagatgaagc cataaggagt tgcgaattgc gaattgcgaa ttgcgatgtg cgaattggaa
    12241 acggcgatgt gcgatgtgcg aattgcgatt ctgcgattaa tacagcacat cgcaaatcgc
    12301 aaatctcaaa tctcaaatct caaatcttaa atctcccggc gaggcagcgg gccatctcct
    12361 cctgcatctc gcgggccttt tctccggcaa cggcgtcaaa gttctcggat gaatctgcgt
    12421 agatgatacc ccgcgatgag tttaccagca ggccgcattg attgtttatg ccataccggg
    12481 ctacctcctc aaggctgcct ccctgtgcgc ccacaccggg cacaagaagg aaatgatccg
    12541 gcacaatctt acgaatgtca gcaagcatct cggcacgggt agcacccacc acatacatca
    12601 ggttgtcagt ggtgccccat ctctgtgaca cattcagtac cttttcaaag agcctctgtc
    12661 cggtgccggg atgatactgg aaatcatctg cacccctgtt tgaggtaagc gcaagaatga
    12721 ttgcccattt tcctccgtag gaaagaaacg gcgtaacaga atcttctccc atgtatggtg
    12781 caacggtcac cgcgtcaaac ggcatctctc caaaaaccgc cctcgcgtac atcttcgagg
    12841 tgttgcctat gtcgccccgc ttggcgtcag ctatgatgaa gatatttgtg tcgagcttgt
    12901 tgatatacct cactgttttt tccagtgctg ccatgcccct tgcgccatat gactcataga
    12961 aggcgaagtt tggcttatag gccacggtat acctgtgagt ggcatctatt atcctcctgt
    13021 taaactcgaa aatcggttct tccaggcctc ccaggcttgc cggcagtttg gatatctcgg
    13081 gatcgagccc tacacaaagg aaggacttct tattaagaat atgatggaat aattccctcg
    13141 aggtcatgat aacacatatt aatagttact ctctttcaga cgctcagcgt tctcagccat
    13201 ctgcagcttg tcgagcagct cctgaatgtt cccgtcaaga actgacggca ggttgtaaag
    13261 ggttgtgttg atgcggtggt cagtaacccg gccctgcgga tagttatatg tgcgtatctt
    13321 ggctgacctg tcgcctgttg ataccatcgt cttgcgcttg tgcgaaatct cgtcgaggta
    13381 cttctggtat tcaaggttat atagacgcgt gcgaagctcc ctcatcgcct tctcgaggtt
    13441 cttgatctga gacttttcgt cctggcacgt taccacgacc ccggtgggga tatgtgtcag
    13501 cctgattgct gaataggtag tgttgacgct ctgtccgccg gggcccgatg agcagtaagt
    13561 atccttgcgg atatcctcgt tgcggatctc tatgtcgaac tcgcctgctt caggaagcac
    13621 cgccaccgtt gcagccgatg tgtggatgcg gccctgtgtt tcggtctgcg gcacccgctg
    13681 aacgcggtgg actcccgact catatttcag gatgccataa acaccgtcac ctattacgtt
    13741 gaagactatt tccttgtacc ctccggcagt gccttcggtg taatagttca tatcaacctt
    13801 ccagccctga gcctctatat atttcgaata cattcggaac aggtctcccg cgaagatact
    13861 tgcctcgtca ccccccgtgc cggcacggat ctccatgaca gcattctttt cgtcctccgg
    13921 gtcagcaggc aggagaagat acctgatgtc ctcctccata tccggcagag actgcaaaag
    13981 ggtgtctatc tcggccttgg ccatctcacg catctcctca tccttttcgg cagcgaggag
    14041 ttcccttgca ctctccagat tgtcaatagc ggatttgtat ttctcatacg cctcaaccac
    14101 aggtttgagc tccttgtatt ccttgttaag ctttatatag cgcttcatgt cagacattac
    14161 gtcgggggag gtcaggagct cacccacctc cacaaaacgc ttctttacgc caatcagcct
    14221 gtcaagaata agattgtttg ccatccgatg caaaatttca gggtgcaaag gtaataatat
    14281 taattgcgat ttgcgatttg cgatttgcga tctgagaatt gagaattgca aggtgcgagg
    14341 tgcgattgca gatgaccagg cactgaagaa ggtcagtaaa taaatttccc ccgatcagta
    14401 ctgattgtca gcccccggga ttcggctgcc tcgcagcggc caacaacacg ggcatcaagg
    14461 ttgaatgagg aagcaatatc aatgattgcg gcagcgtcag cctcattaac atacagctcc
    14521 atgcggtgac ccatattgaa cacatggtac atctcctccc acggtgtgcc ggaacttttc
    14581 tgaatcagtt caaacagcgg gggaaccgga aaaagattat ccttgaccac atgcaggtta
    14641 tcaatgaaat gcatgatctt ggtctgccca ccgcctgagc agtgtaccat cccgtgaatg
    14701 gcaggcctca taacgtccag caccttcctg atcaccggcg cataggtgcg tgtgggtgac
    14761 aggaccagcc tgcctgcatt gatgcccggc accgctgtag gatcagtaag gcgcatcggc
    14821 ccgctgtaaa cgagctctcc gggaatacct cggtcatagc tctccggata gaggccggca
    14881 aggtacttgc cgaatacatc gtgccgtgcc gaggtcagcc cgttgcttcc cataccaccg
    14941 ttatactcac tctcataggt tgtctgtccg tacgatgcaa gccccacgat aacatctcca
    15001 gccctgatgt tcgcattgtc aatgacatca gcccgggcca tccgggccgt caccgtggag
    15061 tcaacaatta ccgtccgtac caggtcaccc acatcagccg tctctcctcc cgttgaccat
    15121 attccgatgc cggcgtccct catccgggca aggaatttct ccgtgccttc gatcagggcc
    15181 ttaatcacct caccgggaac aaggttcttg ttcctgccga tagttgatga cagaagaatg
    15241 ttttcagtta ctcctacgca tagcaggtca tcgagattca tcaccacggc gtcctgggct
    15301 atccccttcc agactgacag gtcgcccgtc tccttccagt aaatatatgc cagggacgac
    15361 ttggtccctg cgccgtcggc atgcatgata ttgcaccagg caggatcacc tcccagcatg
    15421 tcaggtatta tcttgcagaa cgcccgggga aagagaccct tgtcaacacg gctgattgca
    15481 gcatgaactt cagccttacc ggaagaaact cctctcaatg catagcgtga tctgtcagtc
    15541 ataatcccgg ccccggatct taattatttt gcaggctcat agtcggtccg cagcaccctg
    15601 aactcggtac ggcggttgac ctgatgggca atctccttct gctcatcgct ggcgaggccg
    15661 ttgatgtact gctcggtgag cgaggcaccc aggcgcagga agggagcatc cttcatgatc
    15721 tcatcatcga ccaccttcgg actggtttcg ccataacccc ttgcagtaag cctgtcagca
    15781 gctattccct tgcctatcag gtaatcaaca accgactgtg ccctcttctg tgagagatcg
    15841 aggttaaact cctcggtgtc acgggagtca gtgtgcgaca taagctcgat ggtcactgtg
    15901 gggttgtcaa cgagggtctc caccagcttg tcaagtgaca ccattgattc aggcctgagg
    15961 tcccacttgc cgaagtcata gaatatattc ggaagctcaa taggtctgtc gatgggggtc
    16021 atctgtatgg tggccatgaa gtcacggctc ttgtcctgtc ccctcgtagt ctctttttcc
    16081 ttcccgttga ggtagccctt cttcgaggca aggaagatat agtcaacctc cggtctcagg
    16141 ctgaacctga agtcgcctgc attgcctgtc tcggtctgca tgttggcgcc gtcgctggca
    16201 atgagctgca cggtggctcc ctgtacgggc tggcccgttt tttcatcctt gacgaggccg
    16261 gtgatgctga aaagcagggg aggcatctcg aaggtgaaga ggtcatcatc gcccctgccc
    16321 ttgcgtgctg agctgaagat acctttctcg gcatcgcctt cgaaggtgat gccgaagtcg
    16381 tctgccgggc tgttgatggg tggcctcatg ttctcaacgc tccagctttc gccgccctgc
    16441 ggggtagcct tgaagatatc gagtccgccc attccgatat gtccgtcgga ggcaaaataa
    16501 agtgttccgt tttcgcgtga gaaggggaag agctcattgc cgcgggtgtt tatgtcagtg
    16561 ccaaggtttt ccgggcgtga ccatgcgctt cccggcgagg tgcgtgtagt gcggtatata
    16621 tccttgccgc caagtcctcc cggcatatcg gagacaaaat agagagtctg cccgtccggg
    16681 gagattgagg ggtgggctgc aacaagggag tctgccagga gttcgacctt taccgggtca
    16741 ctccagcggg taccttcgcg ggttgaatac ataatgacac atcccttgct ctcgcgcttt
    16801 ccggcctcgc agcgggtgaa ccacacttct gagtaatcag cagagaatga aggtgttccg
    16861 tcttcgaact cactgttcag aaaatcaaca ggcaccgggg tgctccactt gcctttcttg
    16921 tcaagcctgg tctcaaagat atctgtgaaa ctctgtccgg tggcgccgtg ttccttgtta
    16981 cccctggcat catcgcgtga ggaggtgaac cataccaggc cgtaatcatc gcggccatag
    17041 gcgggactga aatcagagtc tccggtattt gcttccttca gttcttcgac gttataggat
    17101 tcaggcgccg actgccactg tatggcaagc tcgcaggagc gtatctgctg ctcggtacgc
    17161 ggatctcccg gcacaagcct gcggtattcg cgaaactcct ctatggcctg gggataccgg
    17221 cccagcttct tgttgctttc tgccagccag tactgtgctt caggtttcgg gaaaggtgac
    17281 ctgacagctc ttttgtacca caactcggca ttcttcgggt cattgatgag ccggtagcac
    17341 tctgacacca tgaacaccat gtcagctttc agctgtttat cacgtgtctt cgaatagaca
    17401 tccttgaaat agtctattgc ctcaaaatag ctgccggcat tgtatgcggc gtatgcccgc
    17461 tccgtcttct gcttctgtgc ctgaagcacg gatgggcaga gaagggtaac agcaattatc
    17521 agtattatta ttctgttcat tccggacacg atagtgtttt gtacaaaagt aatgattgtt
    17581 attaatgtta acgttcagcc gtttcattaa ttaccggcac ccggaatctg cagcaaaaat
    17641 aatgccctct tcacagaggg cattggaaac aaaatttata taactaaaca acctgttacc
    17701 gggtaaacag aagctcacgg tatttgggaa ggggccacag ttcgttgtct acaacaagct
    17761 cgagcttatc aatatgggtt ctgatttcct cgagataggg tttgaccttt gactcatagg
    17821 caagcgcttt cttgtacagg tcagggataa catttgcctt cctgcgctga tcgatcatct
    17881 cggtgaccag ttttttgatc atggaaatat ggtcagaaat tgttactatc agttcctttc
    17941 gcgcacctgc cagccgtatg aactcattct cgtcaaatat ttcacggaga cccttgacgt
    18001 tatcaaccag cgaggtcatg tagttcactg ccgtaggcac tatgtgattt atagccaggt
    18061 cacccagcac gcgggcttca atctggagct tcttggtgaa tttttccagt tcaacctcca
    18121 ccctgctttc cagttccctt gcagttaaca ccccggttcc caccagcgtt tcacgcgaag
    18181 atgagttctc gtaagtcttg agggtcttcg ggacgcttgg ttcatccgag aggcccctgc
    18241 gtattgcctc tttgtgccac tcagcgctgt aaccgtcacc atcgaaacga atcctcttcg
    18301 agtcgatgat gcaatttttc agcacctgga agattgcttc gtctctcttt cctccgcggt
    18361 caattattgc attgacctca acggcaaacg ccttcagctg gcgtgccacg gccgcattaa
    18421 gcactatcat tgcagagctg cagttggccg aggagcccac cgccctgaat tcaaacctgt
    18481 tgcctgtgaa ggcaaacgga gatgtacggt tgcggtcagt attgtcaaga aatattcccg
    18541 ggatcttgcc aatgcccagc ttgatctccg tcttctcctg cggggtcatt ttcttgtcct
    18601 tgactttgct tgcaatgcca tcaagtattt cggtaaggta gctgccaagg aagacagaga
    18661 tgatcgacgg gggtgcctca tgacctccca gccggtatga gttgttctca tttgtaacac
    18721 tggcgcgaat cagttcctgg ttgtcattta ccgccttgat cacgttgacc aggaatgtga
    18781 ggaactgcat gttcgtcttg ggattcttgc ccggagagag gagatttaca ccggcatcag
    18841 tcatcatcga ccagttattg tgcttgccgg aaccatttac ctcggcaaag ggtttctcat
    18901 ggaacagcac cctgaacttg tgcttccttg caacccttct cataacgtcc attatcagct
    18961 ggttatgatc gacggccagg ttggcttcct caaacacggg tgcacactcg aactggttgg
    19021 gggccacctc gttgtgacgg gtcttaaccg gtatgccaag cttgtaggcc tcgtactcga
    19081 actccttcat gaaggccttc accctgtcag ggatcgaccc gaaataatgg tcagagagct
    19141 gctgatcctt tgacgagggg tgacccatca gtgtgcggtc agccagcatc aggtcgggac
    19201 gggcataata cagagcctca tcaataagaa aatattcctg ttcccatcca agtgtggcta
    19261 tcaccttttt gacatccttg tcgaacaggt ggcagacctc cacggcggcc ttgtcgagtg
    19321 ctgccagtga cctgagaagt ggagttttgt aatcgagcgc atcacctctg tatgacacga
    19381 agactgtcgg gatacagagg gtatttccga cgatgaatgc cggcgatgat acatcccatg
    19441 cagtatatcc ccttgcctcg aaggtgttcc ggatgccccc gctggggaag cttgaggcat
    19501 caggctcctg ctgcacaagc acctcgcctg aaaagacctc tattacggag cccaactctc
    19561 cccttccgac gaaagcgtca tgtttctccg ccgtggcgtc tgtcagggga tgaaaccagt
    19621 gtgtgaaatg agtcactccg tgctccattg cccacgcctg catgcctgtg gcaacctggt
    19681 cggcaatctg ccggttgatc tgtgtgccct catcaatggc ggttatgacc gccctgtaag
    19741 cctcgcggct taaaaagcgc tccatcttgc gcttgtcaaa gacattctct gaaaaatagg
    19801 tggataccgg cgctgccggg atgctgacct caacaggatc cctgttgaac agttccttca
    19861 atgcactgaa acgtaattct gccatgatct gattttttgt caaaaataga tcatttcaga
    19921 atataccccc tatttttgag ggtatttttt gataatatgc gcgtttttta tcgttaaccc
    19981 cccttttttg aggggttgtt ttgatttttt aatctgtttt tatgattgtg cggcattctg
    20041 cacgggtaaa gatcccgtac tgtgattttt caaaatccac ggccaccacc ttgtactcag
    20101 gggtaagggt gaactcgtca ccgataccgc tggtgaggaa attgatggca gcctcgggga
    20161 aatggaatgt ggtatagatg acgccgggtt tcaccacatc ggtcactttc accttcatgg
    20221 tggtgatgcc gcgcgcagag aagatccttg cataatcacc gctcaccagc cctttggctc
    20281 cggcatcgta cgggtggacg aacagaatat cctccgtcac cagctcgctg ttgggtgtgc
    20341 gcctcgtcat ggtgccgctg ttgtagtgtt caaggaggcg tccggtggta aggatatatg
    20401 ggaatcggga ggcattctca ttcagctcgg gtgagacgtc atagtcccat gaatggaacc
    20461 gtcccttccc ccttttgaat gtttcggtat ggagtatctg cgtgtcagtg ccgtcttccc
    20521 tgacaggcca ctgctttccg ctgttgccaa gctcatccca ccttacacct ttaaaaaatg
    20581 gcactatgcc tgctatctcc ttaagtatta cgtcagccga gtaacccttc tgcgggtaac
    20641 ccatgcggtt catcatgtca accacaatct gcccgtcggg tttggtcccc tccagaggct
    20701 ccaccgccct ctgcaccttc tgtatccttc tctcaccgtt ggtaaaggtg ccttccttct
    20761 ccagaaagga tgatgccggg aagataacgt cagcgatacg ggcagtctcg gtcatgaaga
    20821 gttcctgcac cacaagcagg tcgagtccct gaagcgaact cttgacatga caggtgtttg
    20881 gatcagtctg gagaatgtcc tcgcccataa tccacaatgc cttcaggtct ccccgtgcag
    20941 ctgccgtaaa catctccggg atacggtagc caggcactgc cgggtgctct gcaccgtagt
    21001 aagcgttgta aagggcaatg ttttccggcc tggttacatc aagatatccg gcaccctggt
    21061 gaggctgtat tcccatgtca gccgcaccct gcacgttgtt ctggccccgg agtggattca
    21121 ccccggtccc ttcccttcca atattgccgg tcatcatggc aatgttcgag atcagcgtga
    21181 ttgccttggt agcctgccag tgttcggtca cacccagccc atggaactcc atcgctgact
    21241 tcgccgagcc gtactctatg gctgccgccc tgaccttctc acggtcaaca ccgcagatcc
    21301 tctcaagctc atccacgtca agcctctcca gatattgtct gaactcgtcc catccttcgg
    21361 ttctcttcgc tatgaactcg ctgtcaatga atccctcagt cagcaggtaa cgggcgaaga
    21421 ggttgagcac cgccacgttt gtcccgggat ttatctgtag atggtgcttc gccagccgcg
    21481 caatctcaat cttcagcgga tcaatgacga tgaggggagt accggtctcg accaccgatc
    21541 tgaggcgggc gcccgtaaca gggtgggcag atgaaggatt ggctccaata ataaggatgc
    21601 accctgtttt gtatatctca ttaaccgaat tggttgccgc gccggtaccg taagcccact
    21661 gcattcccat tgccgtcggg gcatgacaga ccctggcaca gccgtcaatg ttattggtac
    21721 cgatgactat cctgaaaaat ttctgcatca ggtagttttc ctcgttggtg cacctggccg
    21781 aggagatgcc tgccagagca tcaggaccgt atttgtcttt tatctccctg aatctttttg
    21841 ctgtgtactc ataggcttca tcccatgaaa cctccttcag ttcaccgttt ttcctcacca
    21901 tgggtgaacg aagcctttca ggatggttat agaactcaaa ggcatatctt cccttgacac
    21961 atgtatgccc ccggttggtg gccgagtcga gcggtgcctg tatgcccctg acctgtccgt
    22021 cggcgacctt gacgtcaagg ttgcatccca ctccgcagta ggtgcatgtt gtacgcacaa
    22081 tcctgtctgc cttcagcgtc ttgacggaat aacggtcatg aagggcgttg gtggggcatg
    22141 tctgcacgca tgcgccgcat gacacgcagg gggagtcacg gaagctctgg tcaaatccct
    22201 ttatgatccg gctgtcgtca cctcttccgt atatgcccag cacatgttca gcctgcagct
    22261 catcacacgc tctgacacaa cggtagcatt tgatgcattc tgtcaggtca gaccagacat
    22321 acgggcggct cctgtccttc tcatgatgtg tgacattgcg gtgataacgc acttccggcg
    22381 aaccggcagc ggccacaacg gtctggaact cggtaggcag catgccgggc tcagggatga
    22441 gcctctccgg agggtattcc gacagcacca gttcgaggat attcttccgc agccgcctga
    22501 tccggtcagt tgacggatag acataataac ccggagccac gggagtatga catgatgcca
    22561 tcacttttcc cgggcctccc tccttcagcg ccacctccac ggaacagatg cggcatgaac
    22621 catagttatc aaggttttca gcctggcata gtgtcggaat cacatcatgc ccgctgtttc
    22681 tgcggacaaa ctgcagaatg gtctcacccg gttcaatggc gaaaggcctg ttgtcaatgt
    22741 atgctatctt ttccatggtc atgcctccct tactgtgaag taatccttaa gttcttccgc
    22801 aaaatactgc agggcgttcc tcaccggaag cggcagacca cctcccaggg cgcagagtga
    22861 cccgagctgc atcgtttcga gaagatcatc aaagagctgc cggtcaatct tttctccttt
    22921 ttcaattgcg ccggcaagca gctcataacc ccgtgccgag cccagtctgc aggggaagca
    22981 cttgccacat gactccttcc tcgtgaactc aaaaaggtgt tccagcaatt ttattaccgg
    23041 gaagtctgca ggcaccgata ctattccggc atggccgaga aggaagccct cgcgggcgaa
    23101 cgactcgaag tcgagtgtca gatcatcaat ccgcgacgca ggtacaagtc cgcccagcgg
    23161 tccgcctacc tggaaggcct tcaccgggta tctcgttccg cctccaagct catcgatcac
    23221 tgttctcatg ggggttccca tcttcacctc cagcagtccg ggttttttga aagccccgtc
    23281 gagtgatacg agttttggcc cgggtgattt ctctgttccc attgctgccc aggcggcacc
    23341 accctcctcc aggatgtagt ggatattggc gaaggtctca acattgctca gcaccgtcgg
    23401 tctgccgaag aggccataga cggccgggaa cggcgggcgt gttcgcactt ccggcctcag
    23461 tccctcgagg gagttcagca aagatgtctc ctctccgcat atgtacgccc ccgatccttc
    23521 caccacgtgg aaacggaaac gggtgatctg attgccttcc gtacctgtcg caattcctgc
    23581 cttttcaatc tctgtgacag cctcccgcat cctccggacc gccagcgggt attctcccct
    23641 gatatagact actcccgtat cagcgcctgt gatgatacca gtcatcatca tgccaaaaag
    23701 tacggcgtgc ggccgttcct cgagcagcca tttgtctgaa aaggctccgg ggtcaccctc
    23761 atcgccgttg cagactacat atttcctgtc agctgcggca tcgcgcgatg agcgcacctt
    23821 tacatgaaac gggaatcctg cgcctccccg gccccggagg cccgaagcct caagctccct
    23881 gagagcatca tcagtcctcc cggtccattt ctctgcaagc gaccagtaat ttcccgtatc
    23941 gcctgaaggc gtcagaagtg caggcatgtc tgcattcgag ccaatgctca ttgatgacgg
    24001 atcggcatca cttccgcaaa gcctgatatt tccattgaca agaaatgcgg tgtttgagtg
    24061 acagtgaccc aggcatgtca tggtgcctat ctcatcctcc ctgtaacttt caagcaggct
    24121 ccgccggaca tcctcgctcc ggcctgatgt aaggcaggca gtgccgtcac acatgaatac
    24181 ctttttcccg gagtgggcag gcctgagaaa atcatagaag gtacttgaac tcaggatggc
    24241 tgcatcaccg gtcatgaatt ccccggcaat ggccttaaga ttctcattac gagcattatc
    24301 atcatcgccg gagactccgg caatactctc gtataagctg tcggtaaggt ctcgcctggc
    24361 agagagaccc ctcaggttcc ttgacatggt tatactgttt ttacttttcc aagatattta
    24421 ctaactgtgc gatatgaccg aaatatgaga aatatcatat ttcccggaag ttaaataaaa
    24481 tgagaagaag gcccgtcctt ttgtttaact gaattttgtt atatttgtcg cctgtatttt
    24541 tcaagaggta aaattcatta tttaattagt attcagatgg caatacttca gagtttaaga
    24601 gagaaggcag gaccgctgct ggcgattgtg attgggtttt ccctgcttct ctttgtggtg
    24661 agtgattttt tcggaagtaa cacttcccag cgccgaaagg caaacaaata ttatcagctt
    24721 gccactattg acggggagac cgtgtcatac caggacttcg aagcccgtgt tcaggaactt
    24781 gtagatattt acaaaatgtc aggatcatca gaagtcaatg atgaaatgat gcagagcctg
    24841 agggaacagg tatggcagca gatgctctca gagaagctga tgggcaagac ctaccggcag
    24901 gttggccttg gcgtcagtcc tgacgaagtg gagatgctcg tctttggtga cgacccccat
    24961 cctattgtca ggcagctttt tactgacccc ggcaccggca ccttcaatga atcattcctt
    25021 gtgaattttc tgaaggccac cgagactgat gacgcaacca aaagatactg gctattcttt
    25081 gaggatcaga taatcaatga caggctcaat accaagctgg tcaacctgat gtcaaagggt
    25141 ctctatgtca ccggcaagca gtcggagttt gagaacagcc taaacgggaa cactgtcagt
    25201 ttcagctata tggtacgcaa ctatgcggcc atacctgaca gtctggtgaa ggttaccccg
    25261 gacgagatca ggagctatta tgacaagcac aaggataact tcaagcgtgg tgccaccagg
    25321 gatatggagt atataacctt tgagattgct ccctcagagg cagacatgaa ggagaccgag
    25381 acctgggcca taaatgagaa ggagaacttc gctacagcca ctgacgtgat tcagtatatc
    25441 aacctgacgg ctgacaccag gcacaccggt ttttatctga caacggagga tctgcccgaa
    25501 agcctgcggc cgctggccga ggcaggtgac aaatctgccg ttgccgggcc ttatcttgag
    25561 aacggcagtt acaagctggc acgcataatt gacatagccg agcgtcctga ttctgtcagg
    25621 gcagcacata ttctgctctc acccaaccca accaggcccc ttgcccaggt aagaaaagaa
    25681 gctgacagcc ttattgcact cgtcaggtca gggattgact tcaacgtact ggcaatggcc
    25741 aactccgatg accagggttc ggcacaggta ggaggcgatc tcggatggtt cagcgaaggg
    25801 atgatgataa tccccttcaa taatgcctgc ttcagtaata agaagggtga cattgtgact
    25861 gcagagacaa cctacggcat acatgtcatc aagatacttg accagagccg cagggtaagg
    25921 aaatatgaca taggcatcgt agaccgccag gtcatcccca gcagcatgac cacgcagcgc
    25981 atctattccg aggcatcaca gtttgccggg accaatgaca catacgagaa gttcaacaag
    26041 gctgtggctg aggggaacct caacaagaaa cttgcgttga acgtgactcc tgaccagaag
    26101 gagcttcccg gcctgacaca gtcaagagga cttgtcatgg ctctcttcca gaacagcaag
    26161 gcgggcagca ttgtccttga caacagcagc caggcagtgt tcgagcttcc tgaccagtat
    26221 gtggttgctt actgcagcag ggcccaggag gagggagttg cacccgtgga ggatgtggct
    26281 tcagatatca ggttcatcct ggcaaagaag aaaaaggcgg aggtcattgt tgccgagatg
    26341 aagaagcttg aagccgaggg taaaaacctc aatgagattg ctgctcatta caacaccacc
    26401 gttcaggatg ccagcggaat caacttcagg tcatactcaa ttcccggcgc cgggatagag
    26461 cccgccctga tatcggcagc atcagcctct gaggccggta aactttcgaa accggttgaa
    26521 ggcaacaacg gcgtttacat attcatggtc agcgacgccg ctcccgtggt ggccgaagaa
    26581 ctggcaatgg tgaaggagag aatgaactcc agctaccaga tccgcgcttc atacgaagcc
    26641 taccaggccc ttcgcgacaa gaacgaggtc aaggatatga gatacaaatt ttattgatat
    26701 ccggtatata aattttatat ggtagcggca ggtctgagac ctgccgctac gtgcataata
    26761 tataatatct attttcattc cttttcccgc gacagggaac tgacaaatcc ggcagggagg
    26821 ttgtaaacat ccagcatgat cagcccgtca aggcagttat tgaaatcagg gtcaacattg
    26881 aagcctatga tccgggcgtt gacctcgagg tatttcttca gcagcaccgg tatgcggtaa
    26941 ccattgtcca catcactcac caccttttcg accttgctga tgtccctttc cgctgatgag
    27001 atgatgatct cactgtcaat acgtttgtct atctgggggc tgaacttctt cctcggcctg
    27061 atgtgcatgg ccatctcccg gtcaaagaag ttgttcctga caaattcgac tatcagggat
    27121 ttggaaaacg ccgagaactc attgctgatg cttaccgggc ctataagata acgatacccg
    27181 gcattctgca gcagtactga cagaagaccc ttccagagaa ggaacagggg catcgccttt
    27241 ttctggtact cctgcactat gaatgaccgt cccagctcga gagactcggc caggacaggt
    27301 tcaaatgact tgcggatcct gaacagggag ttgatataaa aacccttgac accatactgg
    27361 ctcatgatct ctcttccctt tccaatgcgg tatgccccca cgagtacccc ggctgcctca
    27421 tcccagataa ccaggtggtg gtagtaaaaa tcatattcat ccaggtcggt tgccctgttg
    27481 gtcccttcac caactgccct gaaggtcacc tcgcgcatcc tgccgatctc cctgatcaga
    27541 ttcggtatag cccgtgtagg ggcaaaaagg acaacaaagt tactgacggt gaacagttgg
    27601 tatgttcgcc ttatgttatt aatctcagcg gcaagtgttg cccggtcaga cggaggggca
    27661 accggttcct gccgcctgtt gcgccgtggc atgaagccgg aaaagaaagg tttggcctcc
    27721 agggatgaac cgagcgaata ggtgcgggcc ctgaggtagc gtccgaaacg ggctatgtct
    27781 ccgaacccgg cctgctcact gactgacagt gaccgcccga tgcgtaccct gatgatcttg
    27841 tgccgtttgt taaacagttc cgagcccagc tttgcagtgc tcagcaacgg gtgtatgcgc
    27901 cccagtatat gataccacct tgaattggta ccgtgaaagt agaccggaat gaccggcacc
    27961 tcagccatcc ttacctcacg aagtatctgc tcctgccact ccatgtcagc tatcagtcct
    28021 gattccgact ggtatgttga gacctcccct gcggggaaaa gagccaggcc gtggccctcc
    28081 ctgacatgac tcattgcctc ctccatccct tttcgggatg tcattccttc atggctctct
    28141 ccttccgcat cggcaggaac gataagatgc ttcagcggat caatcttctg cagcaggaag
    28201 ttggccatca ccctgacatc agaacgccgg cgggcaagta tcttcaaaag tatcagggcg
    28261 tcaattccac cgaagggatg gtttgagacc gtaatgaagg ggcccgtcag cggaatattt
    28321 tcgaggtcct tgtcgggtat atcgtacctg atgtcaaggt ggtcaaggat ggaattgata
    28381 aagactgccg gatcattgtc ataggtattt gtgtagaccc tgttcagctt gttatagcga
    28441 aatatctgca tcagggcttt ggcaatcacc accccgcccg gtatgtcaag attggcggcc
    28501 ctggccagct cccttgaggt cagtagtttc atgagctgct ttttctatta tatgaggaag
    28561 ggcatcttcc cagcccagtg gcattatatg tacgccactg attccctttt tggacttcag
    28621 cctcaacagt gtctcaacgg tgatggcgat gctctcttcg gcgaaacgtt caccggcatc
    28681 tgccagtctc tgcatcaccc tctccggcac tgtcactccc gggattttct cattcaggta
    28741 ccgggcaatg gagagcgact tcagggggct gatacccaca agaatgaaaa ccttatccag
    28801 gaggttttcc atgtcaagct gttccagcca tggatcgagg cggtcaggat caaataccag
    28861 gtttgtctga aggaactgcg ccccggcatt gactttttta cgtatacgga tggcctgcat
    28921 gaccggatcg agtgcaaatg gggctgcagc agcgccgagg aagagcttcg gcctgttctt
    28981 tatctccctt ccatcgaggt aaatgccttc atcgcgcatc cttctcaata tccagagcat
    29041 ctgcaccgag tcgacgtcga tgatgttcat gttgctccgt ggtgcgggtc cgacccgggg
    29101 gctgtcgccc gagaggcaga gtatgttgta cacccccagg gcattggcgc caatggccag
    29161 agattgcagg ttgttgcggt tgttgtcgcg ggccgagatc tgcatcaccg ggtcggcccc
    29221 gagtgaggct gccacggcgg agcaggcagc gcttgacatt ctcggtaccg ctgatgaccc
    29281 gtcagtaaag ttgattgccg agacataagg catgaccgtc agtatctttt cacgcagcct
    29341 cgccgtggcg gccgtaagcg gcggcgtcac ctcggcagtg acgacgaacc tgccgctgcg
    29401 caactctgcc tcgagtgccg actgaggccc ggaaactacg ggaggatggt actcggtgtc
    29461 acctttccac cagtcgggct gtcggatggg tttgaagaca ctttcctcaa gcatgttgcc
    29521 cattgtccgg cctttcctcc gggaggggac aagaattttc ccgatgcctg tttttcgtgc
    29581 cgtgcgcacc acttcgcccc atgtctccgt tccggccctg tcccagtcaa cgggtggcag
    29641 cacctccaga agcctgtgct cacgatgcat gcgtcgtgac cgctcataaa tcaggtgcca
    29701 ggcgcagcgg cgtgcggggt caacatagca gtagccattg ttcaccccgc cgcatgggcc
    29761 gttgcggatc cccttgggac acgccatatg acagataaat gctgtctcct gcagcaggca
    29821 gttgccgcac atcctgcatc cgaagagcgg acctttgacg atcctctctg tcagtgcaag
    29881 caattgcttt ccggcaggct gcctgctgaa aggacggaat gcaggctgcc agcgccggag
    29941 gggcgtgaag acagccattg atctgtcaga tgatatacat gttaatcagc tgctcataaa
    30001 gctcctgctt gccgctgatc tttttcggct caccaacctt tttggccagc tcacgaagat
    30061 ccccgaggga cagtttgccc ttctcaaaga gagcgccgct gcccttgtca aatgaagcat
    30121 accttgcttt acgcatcttc aggtaatctg aatccttaag gatgcggtct gcaattacca
    30181 ggccacgggc aaaggtgtcc atgccgttga tgtgggcaat gaagagatcc tccggatcag
    30241 tggagttgcg gcgcacatgg gcatcaaaat tgatgccgcc ggttttgaag cctccggcca
    30301 tcatgattac aagcatcgct tcggtgatct cgtttatgtt tgtcgggaac tcgtcggtat
    30361 cccacccgtt ctggtagtct cccctgttgg cgtcaatgct gccgagcatg ccggcatcgg
    30421 ctgccgtctg caggtcatgc tggaaagagt ggccggcaag ggtggcgtgg ttgacctcca
    30481 cgttaagggc gaagtccttg tcgaggccgt agtgcctcag gaagccaatt actgtggctg
    30541 aatcatagtc atattggtgc ttggtgggct ccatgggctt gggctctata tagaacttac
    30601 ccttgaagcc gttcttgcgg gcatagtcac gtgccatggt gagcatcatt gccaggtgct
    30661 cgagctcacg cttcatatca gtgttgtgaa gagtgaggta accctcgcga ccgccccaga
    30721 agacataacc ctgtccgccc agcgcaattg ttgcatcaat ggcattcttt aactgtacag
    30781 caacgttggt gagcacgttg aaatcaggat tggtggcagc gccgttcatg taacggggat
    30841 tcccgaagac gtttgccgtg ccccacagga gtttcattcc tgtctccttc tgcctggcct
    30901 taatgaccgg cacccacttt tcgagacgct tttcaatctc gaagactgat ccgtcgtcac
    30961 caacaaggtc gacatcatgg aaacaataat agggagcgcc tatcttttca aagaattcga
    31021 aagccatgtc aagcttgacc ttgatcttgt catcgccttc caacccgtca tacgggaaat
    31081 cccttgtctt cgcaccgaag gtgtcagtgc cgtctccgca gaaggaatgc cagtaagcta
    31141 tcgcaaagcg aaggatatcc tccatcttct ttccgcccac ctttgcctcg gggtcatacc
    31201 atttaaatgc caggggattc tttgagtccc ttccttcata ctgaatcttc ccgataccgg
    31261 gaaaaatttc gcttttactt ttttttgcca taggtttaaa attattcata aagatgattt
    31321 tcaagaatat cgagccactc attatatctc gcgtcataga tcatagccag gcggggatcg
    31381 ggttcgcttg agcctgtcac ctcgaggccg ctgaaggcct cctcatccgt cctgtagaaa
    31441 ccgcatccta tcccggcgcc acgggctgcc ccgacggagc cgtcggtatt gtacaaagtg
    31501 atcactgtcg aagtcaggct tgaaaggacg tccctgaata ccttgctcag gaacatgttt
    31561 gactcaccgg cacgtatcac cgtgggatta atgcctgtct ccttcattat ctcaaggccg
    31621 aagcggaatg agtaggctat tgcctcctgt gcggcacggt aaagatgtcc ctgccggtgg
    31681 atattgaaat tgaggccatg taccgaagct cctatttccc tgttacggaa catcctttcg
    31741 gctccattgc cgaaggggag gatgatcagt ccgtcacttc cgggctccac tgcagcagcc
    31801 agatcattca tctcagggta gttcagcccg gcgccggtat tcttcttcag ccaggagttg
    31861 agaatgccgg ttccgttgat gcagagcagc acccccaggc gcgtcagcct ttcggtatgg
    31921 ttaacatgca gaaaggtatt gattcttgac agcgggtcaa actgggttct gtcagtaaca
    31981 ccgtagatta cccctgaggt gccggcagtt gtagccacct ctcccggttt caggacgttg
    32041 agtgacaatg cattgtttgg ctggtctccg gccctgtagg ataccggtat gccgggagta
    32101 agcccaaggt cggcagctac tgcagccgtc agctctccct ggagggagaa ggaaggaacg
    32161 gcatcagcaa gcaggtcagg tgagattccg taatggtcaa gtaactcttc cgatatctca
    32221 tttctcatga aatcccagaa aattccttca gaaaggcctg aaacggaggt ctttatctca
    32281 tttgtcaggc gcatggccag gtaatcaccc ggaagcatga tcttgaagat ctgtctgtaa
    32341 atatccggtt cgttctcctt tacccaagca agctttgatg ccgtgaaatt tccgggagag
    32401 ttcagcaaat gatgcaggca gaaatctttt cccagcctgt caaaagctct ttcaccggtc
    32461 tccacggccc ggctgtcaca ccatattatt gaaggacgga gtacctttct gtttttgtca
    32521 accaccacca atccgtgcat ctggtatgaa atgccgattg cacctatgtc cttacgtttg
    32581 gttcccagtg gcttcaggca tgctgccaat gccagggtga agtttttcca ccacagttcg
    32641 ggatcctgtt cggcccagcc gggggcaggt gcggaaatct ccatctctga tggaggataa
    32701 gaagctgagg agatagctct ccctttttct ccgtcaataa cggagacttt tactgatgag
    32761 ctgccaagat ctataccaag taatagcata cgggatttgt attaggaaaa atttcttact
    32821 gcggtgataa atgtacggat attttccgta gaagcatcag gaggcattcc tcccccgcaa
    32881 gaccagataa ttcccctggt gtcaccggcc tcgcgcatca tgctgataac gcctgcctcc
    32941 acatcggccg gagtacccat ggcaagcaca tcccttggag gaaggttgcc cagaaggatg
    33001 acgccatcac cggataactt cctcatctca gtaatgctgt gattgaagct gaagttaaag
    33061 aggtttatgc ctatttcctt caggtatgga gctgttacga ggcctgcagc atcattgtgg
    33121 aagaatctca ctttcggatt gaatgttgag tagatgcgct tcaggtgagg cagcacaaag
    33181 gtctcaaaat caggctgccc caggaagccg acaatgtcat cgaggagcag cactccctca
    33241 atgtcaggga acttttcata ctgcagctca agccagtcaa tgacaaagtc agtgattacc
    33301 gtaagcgctt tctgcgcctc atcgggagtg agcgccagcg ccatcatgaa gtcagttgtg
    33361 ccgaacagaa acgaggcaat gttaagaggg ccccggctgg aggcaaaccg gtaatgacag
    33421 ccgtactgct taaccctgtc ctggtatttg gccatacggc ttatgacaaa cggaaggagc
    33481 ccgtcggtct tcacgcatgg cttcgccagc gaagcaacag catcggtggt gcagttcagt
    33541 ttcaccggat gtggaaaatc gttctcggtc cataccatct tcgtgccgaa ggcagagggt
    33601 tcggtgcaca taccgtactc tgaccagaaa ccgggaatga acattacgtc aggaaatacc
    33661 ttcatggcat gaaggtttgc ctcgaaccat gttgcagggt cacagaagta ttcaagtata
    33721 gtggtgccgt accagccggg aagccaggga ctgtctataa taaatcccac ggggggttcc
    33781 tgcgtttttt ctcctcttat catcttaagg agggtatccc agtgtctgtc attcataagg
    33841 tcaggttatg cttcgttata ggatattcag atgcccggga cataatggaa cttcaatacc
    33901 cggtaatatt ccaggctatg ctgtggctgt tccaccccat cagggaaggg catccgtgaa
    33961 aatgtctgaa aataaaggag acatgcgtcc ttccaccacc gtgcctcatc tgcctgtatg
    34021 tcaagcatct gccgcacgtg ttcaaactgc ccgtcatcaa tccgccccct cagggactcc
    34081 cataatgtac ggtattcatt aactgtattc acaccttcct ggtatttcag gcacatttcg
    34141 tcccacagag tccttcctga tttcatcacg tgataccatg gcaggtggtg gaaccataaa
    34201 agcaggtttt ccgggcatgc ctgcggatca ttgaacagcg agtcaagggg agggaagtat
    34261 tggctcacgg catcacttcc cgaggtggtg cggtcaaatc ccactccggc cgtgtcagca
    34321 cggtgataat agaccgacgt ccagtcgggg cgtccccggt ccacccacgg gcccggtccc
    34381 cagtgatgcc cctcggccat tatgtggtgc aggcccaggg gggtcatgta attcactgct
    34441 gcttcgcgtg aaccgagcat catttcctct attcctccgg ttatgtccgg atcgtgtgtt
    34501 attgtcatcc tgatccactc tgatgctatc cctgcaggtg acagctccgg attccaggcc
    34561 agcctgccga aggcatacca gttggcctgg ccgaagaggt gtccggtcca gttcctgtca
    34621 ttgccgatgt ttgctacccc ggctatgccg gtcagggtgt ggttgtgaag actgccgtca
    34681 atcaccttcg agacctccga tccggggccg gcagcgtatg tgtcgctgtc cagcacctca
    34741 ctgaagaggg gtgcaaggta tacgaggtga gtggcacagc cgagatactc ctgggtaatc
    34801 tggaactcca tcatcagggg ggtagatgtc atggcgccga agagcggatg gaagggttcc
    34861 cttggctgaa aatctatggg gccgttcttg acctgtacca gtacgttgtc cctgaatttg
    34921 ccgtcaagcg gcacgaattc gttatatgcc tgttttgccc tgtcaacagg cacattgttg
    34981 tcatatacaa atgctctcca catgaccacc ccgccatatg gtgcgacggc gtcagcaagc
    35041 atgtttgcac cgtcagcatg agaccggccg taattctgtg gaccgggctg accctcggaa
    35101 ttggccttca cgaggaagcc accgaaatcg ggtataagtg aatagatctc tgccgccttc
    35161 tccttccacc aggctgccac ggcagggtca agcggatcag atgtggggag gttacctgtc
    35221 tcctgaggag cgctgaaacg ggcagtcaga taaaccttta tgccgtaagg cctcataacg
    35281 tcagccaggg cggccacctt cccaaggtac tgaggagtaa gaaccagtgc attggcgttc
    35341 acgttggtga ggactgtccc gttgatgcct attgaggcat tagcccttgc ataatctgtg
    35401 taccgtggtg aaatataacc tggcagaaga tgccagttcc agattgagcc gccggcataa
    35461 ccgcgctcaa cagttccgtc gagattgtcc cagtgattga gaagccgcag tgcggtggcc
    35521 ggacgctcaa ggatgtcaag gttgcctgtc ggcgattcgg tgctgatgat cctgagaaga
    35581 tgaaacagac cgtacaatac cccgcggtcg ccctcggagg caaccactgt aatgtttctc
    35641 cgcccctgcc tgatacttcg tattatatag ccctcctccc ccgtggttcc gggaacgaca
    35701 tcagggagaa gtgaggcaac gaagggcgac gagctgaatg ttcctgcaat gacgataccc
    35761 cggccctcaa gggaattgac ctctgtgatt ttcagcccgg tgagctccgc cagacctgca
    35821 atgaactcat catgggcagt cctcagcacc ggtgagttgc ccgttatcat tacattctct
    35881 aaccttctct ggtactcctt caggcgggcc ctgtcagtta cgggcttata cccgagccag
    35941 agccggtagc cgtcatcagc ctgcgccggt gccaccggtg caagaagaag tatcgtcagg
    36001 agaacagtta agattttatg catggctgtt atgattgacc cttcctcagc gatgactgcc
    36061 tgactataag ttcatggctg aggatgatgg agtttacctt ttcatactgt gtgccttcaa
    36121 gaatccttat cattgtggat gcagccactt cgccaatggc cctgcccggg taacgtaccg
    36181 ttgtcaggtt gggctccacg atccgtgaca ccggatcatc attgaacccc accatggcaa
    36241 tgtcatcagg cacccggaat ccttttcgct tcagttcgca tatgagagat actgcagaat
    36301 tgtcattggc tgtgaagatg ccgtcaggca tggtaccgtt attcagcaga tgattgatta
    36361 tggacacccc cgaaccatct cccaggtcgg taattatgag catcttgtca tcaaagttca
    36421 ttgaattgtc catgagcgcc tgacggtatc cgcgatacct gtcattgtaa acgttcctgt
    36481 tcaggcttcc tgcaacatgc acgatttttc tgcaaccctg gtcaaccagg tgttgtgtgg
    36541 cctcatatcc tgccctgtag ttgtcaatta cgacactgac gcagttgccg caatcaaaca
    36601 ccctgtcaaa gaagaccagg ggtatgtcct ttcgcagaat gttggcaaaa gcatcctttg
    36661 tctttgtttc ataggacagg gagacgagca gaccgtcaac gcggctgttg aacagtgcct
    36721 gtatattggc ttcctctagg gcggcagatt cctcactctg actgataagc agctggtagc
    36781 cggagttgtt tgcaaccttt tccattccag agattacagt ggacatgaaa tggctgtcaa
    36841 tccttggaat tattaccccg agggtcctcg tgcgcttctg tctgaggttt gctgcaaact
    36901 tattatgctg gtagcccatc tcctgggctg tatgcattat ctttttcctc agttccttcc
    36961 ttacctgggg actgttgttg aggccgcgcg agaccgttga agcagagata ttcaaagccc
    37021 tggagatgtc atagattgtg acctcgcctt ttttcttcat tccggtcata attcttcaaa
    37081 ataagtaaga aaaccgctat ttttgaaatt tttgaactat ttttttgcaa tcggttgcgg
    37141 atttaaattt aactgactgt ttatgcaacc ggaaacaata aaatcatctg ttaaagagaa
    37201 gattggctac agttccggtg acagtgcaat tgccttgctg ttccttgcag gccggttggc
    37261 aggagctgtt gccggcccgg ttatacctgt tcctttcagg aatgtttttc tttgagtctt
    37321 ttataaacat catcaagcgt tccgctaaac gggccgggag tctccatttt gattgataca
    37381 agagcagcag caaacttcaa cgactcccgg acctcgtgat ccagtctgtg tgagaggtaa
    37441 gcggcaaatg ttgtatcgcc cctgccggtt ctgccggcta cactgttgtt ggagaatttt
    37501 tcataatagg tttccccttt gactctcgca agcacccctt cggaatgcgt aatcaaaact
    37561 tccggacagc cccatgattc aacgattatt gcagcttttt caagatcatc cgtaccggta
    37621 agaagtttgg cttccacaac atccagtttc agtttgtcca tcattgcagc aatttccttc
    37681 ttgtccgcca catcgctgaa ctcgtggttt tttgtcacgg gattgatatg gcgaacaaag
    37741 ctctgcatat caactgacag gcttgggaaa tttctggctt tcatttcctt catgagtggc
    37801 atgtcaaact cggtatccga aattcccgcc aggtgaacac atgtggaatc caattcggga
    37861 atttccttaa tgtcgattat cccggccgat ttgataaggg tcatttcacg ttcatcgaca
    37921 tttgcggaaa agtgtacaac cctgttatac gaagtttctg cgctgggaat gagataacaa
    37981 tcgatgccca gatctttcat gggctgaagg atctcctcgt ccttcctggc cattttagca
    38041 actacggcaa ccttcctgcc aactcttgcg gcaaccatgg ctccgcataa aacagcgctt
    38101 cccggagcta cacgtggcgt tccaccgaac ggaacgatct catcatagca catgtgcccg
    38161 acaaatgtga tatcatattt tttagtcata ccaaccgact tctgattttt tgtcaatgaa
    38221 ttaatttgtg cttatttact ttattcttcc gagtcactca gcaaccaggg atagaaaggc
    38281 atcaggtctt ccggcccgat tgtcatggtc ttgtttttcg cctgcagatc cttgatgtga
    38341 ttcatcacca ggtgtgacca tacggctccg ggccggggat atgctgccac aatataatgc
    38401 ggacagggtt ctttctcacc ggcttcaagt tccagctctg cccggagatt atccggagtt
    38461 gtcagggtat ttgcatactt aaatactacg cgatccatat gtttgtatac ctccttgctt
    38521 tcctcgacca taccgtaata atccagcaac tgggcaaaaa cataattcgt tgtggtatac
    38581 acatcctgcc cctgttcaga ttcaaccggc tctccatcct ttgttttgca catggcccat
    38641 cccatatacg agcggtttgt gtcataaaca gctttgagaa tctttttaac ccgttcttcc
    38701 ggaattgcgg gaggctctcc aatggctgtc aggtaccata aagcgtcaag ctgattggtc
    38761 catacatccg tattcgtatt tcctgtttca ggatcaaagc aggtaacgta atattcgagt
    38821 ttctcacctt ttttatttac atcctcgcgc cataatttct caaaactgtt ccatgctttg
    38881 tcatactttc cccggatgta ttcctcatcg cccttttcgc caagcatttg ggccattttg
    38941 atcaatgcct ggcatccgcc caggaattgg gttgcatcat acgcatcagc gccgaaaagc
    39001 acaaggttat caaaagtatt tttaacatcg tccggatgac cttcggggat cccgtcgttg
    39061 tcaaaatcaa gtttcagcaa cgaatccagt ccgaacttca tcgtttccca gttcgatctg
    39121 aggaattcca tatcccctgt gaattttaca ttacgtaaca cacgcatcat cagtttggga
    39181 tacagatcga cccagtaatt attattgtac catgcgtaat cactcacgtt gcgaagtgga
    39241 tgacccttgg gcattgcacc gacatcatgg gcgacactgc ctttggtctt gaaatcatcg
    39301 cggatctgcc ttagtgatac accttcgtat tcctcacgat gttcattaaa atgcatctgt
    39361 gcctgcacaa aagaagcgtg gtgatgatac catcgcggtg tcggatctac cgccaggatg
    39421 gaattgataa atgcctggca caggtctttt tcgacctttg ggaaaagcat cagcaatacc
    39481 atcgaatacc aatccacatc cgaaggatcg atatacgcat aatcaaaaca ttccaggaac
    39541 cgggccctct cacctttttc atcatctacc cagacgatag cgttggacaa cggaaaatga
    39601 agttcattca gcatcaacct tgtcagacgc aatgctcctt cacggtcttc cttgtaggaa
    39661 gaagttgaag agatcagatc gaagatccgt ttctggattg taacggtccg tttccaccat
    39721 tgcgggtaac tggtcaatgc aatttttacc aggtccagga ttcgtgtctc accattcgaa
    39781 aagcttttta catattttct ctcaaaagtt gttccgtcaa tataatcctg tacgggaaaa
    39841 tcaagagcca cagcgaaagg aacagtcttt gattcaaatg gtcccaggct aaacgtcaga
    39901 ctgacagctg ctccataatc actgcggata accggctgcc gtttttcata aaagctgcca
    39961 tcgctgctca gcagaagatc accggagtat ttattgaggc agaaataggg ttgggtatcc
    40021 acggcaacgc cgggaacgtc tgtaactgct atcgccatcc tgttctggct ttcattgctg
    40081 cccataatta taccacggat accttccaga cggaactctt catgtttctg ttcttttact
    40141 gcagcagtag ctacaaaaac actgtcctga tcagtgggtt tgagctcctt ttcctggaga
    40201 ttgaccagcg atggtcttgg tattacgatt gtgacctgct gatcgtatcc tgtaacattt
    40261 tcaaccacat attcttcaac accgagtggc atcaatgtct gttccccgcc ggaaagcaaa
    40321 ggagaaatca gtctcctgat gaccttcaca ttttcaaatt tataggcaat ctccagtgag
    40381 ggagttgcaa ttgtcaatgc gatgttctct ttttcaaccg gagcaggata ataattttta
    40441 actgccggga tatcatcggg gagttcagtt tcacccggta atttctgcgt cagcattgta
    40501 caatcatcac ctatacgaat atagggcgcc gagccgaatt gaaccgcttt ctgattcaaa
    40561 aggataagac gaccggcttc gttaaaccgg ttatataaac cgtatggtga aataccgatc
    40621 gaacaggtag gtgcgccttg caatgccaca gtatgcggaa actgaagaca tttggacatt
    40681 ccctggaaat gcactgacat aaaattctct tcataaattt ttgcgatttc agtgccatca
    40741 attgtgtaga tatcctgaat gttgaaggat gctgattttg tcattttatt ctcgcttagt
    40801 gttcgtaatc ataaacataa atggagggca atttgcaggc gccatcaccc tttggattaa
    40861 tctgaacgcc agtgttgaat tttagaatcc tcaccggcgc tcagcatatc agtttaatct
    40921 ttttgcccta aaggacctag cacttccctc cccatacatt cccgggtttt ttctcctgtg
    40981 atttcctggg agcgggatct ggttcttccg gcagattctg ttcagcactc agatcgatcg
    41041 gcatcttagt cgtaggataa ggcttgattg ctccgaaaag gagatcagca gatgctctga
    41101 ttgaatcggg tgtgccggaa aaattacata cgacagcatc ggctaccgta gttgttccct
    41161 tcctgtacgg gaagttggta acgacaacca ccgtatggcc tgcggccttc agcgccctgg
    41221 ccagatgggt attgttgccg cgcttttcaa tacgtgcgta gtagttggtc atgaccacca
    41281 ggtcagcttc ttttgcaagt cgcagtgctt cttcgatgtc ttcctcttca gcatggaacg
    41341 gggcatcgtc gagaatcagg ttagtggaat gcttgaccat ttgctcgcag aacatgtggg
    41401 catgactgta caaatccttt ccgaggaatt cataggggat tgtttgctcg atcaccagga
    41461 ttttcttgtc tttactcagg ggcagcagct ttttatcatc gcggactacc agcagggctt
    41521 tgtgcgcaac ttcccgggaa aagtccctgt tttccttgct tctgacaatt ttggtggtct
    41581 ccttcggatc catcttaccg gctttttcga acagaccctg atcatatttc ctgcagagca
    41641 gacggcggac agcatcgttc acgcgctctt caggcaaagt tccgttctca acggcactct
    41701 tgatgccaaa cagacactgt gagcgggatt cgtcgtcagc tttgagcagg atagtgtcaa
    41761 cacccgcttt gatggccatg gcacatgcga gcgggagtgg ccatttttta aggattgcag
    41821 ccataccgat tgcatcggat actatgattc cgtcaaatcc catttcacct ctcagcagat
    41881 cggtgaggat ctttttcgaa agggttgccg ggtactcttc atcgtaagcc gggaaaatgg
    41941 tatgtgctgt cataagagcc gatgctccgg catcgacacc tgcctgaaat gcagccagct
    42001 caacatcctg catgcgcttc ttatcgccgc ggcacacatc gagcacatca tgagcgtcag
    42061 ttgccgaatc accacggccg gcaaagtgtt tgattgtcgc agtgatacct gcatcctcat
    42121 atccgcggac agtagcatcg atgaattttg agataacgct tacattatca ccaaatgacc
    42181 ggacaccaat ctcaggattc agcggattga tatttacgtc acataccgga tgatagaact
    42241 gggttacgcc aattgcagcc atctgacggc ccatcagatt tgctattttg taggcaagat
    42301 ccacatcccc gatggctgta atacccatcg gaggaataaa ctgaagaaca ccaccggcag
    42361 tataatcgtt cttgaaatca ccttccatat caattgtaac gtgcagagga attcccgaag
    42421 gccggttcat ggcaattttc tgcatattgt ttagcgcttc tgtgagttct tcaggcgtta
    42481 cgcttatgcc aaaggcgcgg gaattgaacc atgtgtgcgc aatgtcgaaa taatcatcgg
    42541 gctttttgaa attcggatca acctgtgagc gtccccaata cagattttta catgtttcaa
    42601 gagcatatgg ttccaggcag atgccgcctg catgcagttt ggtaatttgt tcgatcccag
    42661 atggtgtggg gatagctcct ctccaggtga aggtcagcag ctggccgcat ttttccagca
    42721 ggctcatttt cgcgattttc gctgcaacaa aatcatctct ttcctttgac attgtgtttc
    42781 tctttatagt ttattttctt ttttattagt cacggtctcc ggatgcgatc cggataagct
    42841 tctttatctt tttttcttat atttttttgc catcatgatc atatctgcaa ccacgctttt
    42901 gggctggtta ttacggtcga aaagcacagg ataatccttt ctgccgcgga tcgggaaatt
    42961 gttcagccat gatgtggagt cgttcaatgc ccacagtgtg acgcgatcaa ccacatcatt
    43021 gtgtttcaga aagagttcga acatatcaag tacgcgtttg ttccattcct gttgaactga
    43081 gtcgggcaca ccttcacgat aaggatccat ttcctttgaa taggtaaagc gggtagaaat
    43141 gtttgcgccg gaatacggac ttggaagaat ggaaatatcc cattcagtaa tatgaacttt
    43201 cacaccggca gctttgagtt tcattaagct ggtctctgtt gcttcaagtg taggatcatc
    43261 catgtgcatg tgtccctggg aaccaaccgc atcaattctg catcctgccg ctttgagttc
    43321 ttttacaagt tccacgattg catcgcattt tgccggcgtt tctacattat aatcgttgta
    43381 gtacaattcc gtatccgggt cggcttcatg ggcaaactga aaagcatatt tgatgaagtc
    43441 cttgcccagt atccggtaga atttgctgtt gcggtatgag ccgtcgtctt caaaggcctc
    43501 gtttacaacg tcccagcctt taacacggcc tttatagcga cccacaactg tataaatgtg
    43561 ttgacgcata cgttccttga gaacttccgg ggatacatcc ttaccttctt catctacaaa
    43621 gaaccacctc cctatctggg aatgccacat aaggcaatgc cctattacag cctgtttgtt
    43681 tttttctcca aatgccacaa atttgtctgc atcttcccaa ttgtatttgt cttcctgcgg
    43741 atgaatcggc tgtggtttca tgcagttttc ggcgacaatg gaattgaact cccttgcaat
    43801 caattctgat tctatggggt tattgccgtt aacctgttgc atgttaacag ctactccaaa
    43861 aaggaatttg cctttggtag ccttggctat tgtcttttgc gccatagcgt atatgtttga
    43921 cataaaaata atggcaatta ttgcagtaat agtcttgatt ttcattataa tctatttgtt
    43981 taattataat cggttatgca actacctgtc ccctgcgggc atgcagttct gactcaattt
    44041 ttacctccat ttttttatca ataacataga agaacaataa tccgactccg ataaggaatg
    44101 ggatggacgg atacacactg acaagcattt ttgctcctgt agcgacagtt tcaggctgaa
    44161 taatttctgc cccggcaact gtaccttcta tagggatgta cccatacaga ccgaggatcc
    44221 atgcaacaag ggcgcctcct attgaaagcc ctgccttaag gcctaccatc attgcagaaa
    44281 atattatggc cgttgccctg cggtttgttt tccattctga atagtcagca acatcagcaa
    44341 tcatggccca aagaatgggg atggtaatcc cataagtaaa tccatgcaga atctgcgatg
    44401 tgaacattaa gattacactc tcgggtttga aaaatataaa gatcagaaca aaaatggcag
    44461 cagcaagtaa accatactta taaacatcac gtttaccgta tttatctgcg agcttcttag
    44521 ataatgatat tccgacaatc ataaagatta tccctcctgc attaaataaa ccaaagcctg
    44581 cagaaaccgg gtccgatcca aagaaattga tacctatacc tgacagaccg gtaataatcg
    44641 ggtgaataaa tctggtaagg gcagcttcat ctacgtaatt attaaaataa tacacatagg
    44701 cgcctccttt catcgcaaga gtgacgaaca ccaatatggt taagacaagc attataagcc
    44761 atggtctgtt cttcgaaaga tcagaaaggt catccttaac tgatgattgt tgttccacat
    44821 ccggaacgat acgctctctg gttgaaagaa atgatataat taacattaca gtgccaatga
    44881 ctgacatcca ggtcataact gattcaatcc cggcggcttt atctccatgg cctacatatt
    44941 caataatcgg cagcatgaac acctgtacaa agaactgtgc aaacatcaca gcaacgaaac
    45001 gatacgatga gatactgttt ctttctccca tatcaccggt tatgacaccg ctcagtgctg
    45061 catacggaag attgctggaa gcatacatca acaacaacag agtataggtt gtagttgcat
    45121 agattaactt ccccttgtat gaaaagtccg gcgttgaaaa ggccagtatg gcaataacgc
    45181 caagaggaat agcagtgaat aatatccagg gccggaattt gccccatctc gaattggttc
    45241 tgtcagccaa tatgccaatc agcgggttaa acccaaatgc ggcaaccagg ccgacaatca
    45301 gcattactat cgaggcatcg gtgcttttca gcccataaat gtccgtatag aaataagcta
    45361 aataagtaat cagtgtttgg aaaaccaggt tcgcagcaaa gtctcccaga ctatacccta
    45421 ttttttcgaa cactgagatt ttttgagaag ttaggctcat gcttttttat tattagttgg
    45481 agatttacat acacctgtta atcaacccca ttccacggaa ccagttgatc ccattgaagg
    45541 cggtccaaaa aaaagctttt tcacggagaa aacctttggt aaatctatca atttatttta
    45601 aaattccgtt ttttcaacca tcaaaacaag ggctgaagcc cgggtgatca taacaggtgc
    45661 aggtacccag ggctgaagcc cggacttggt gatgcagtgt tgttatggtt cgtttgaaaa
    45721 gggatacatt gacaattaat cactatcttt gctttaatgt aaaatttatg tggaacgtat
    45781 tgtaaatctg cataagacgg tgctatccgt tttttgaaat cccggcaccc tgattggtac
    45841 aggaccttgt ttgacatggt ccggatgttt aatgaattct gaacttatga aaagactgac
    45901 agtctttgca atttcagcag gaattatcct gcttggccag ttctgcggtg atcctgaggg
    45961 cccgccagat ccggtaattg aacctgaaat aaataaagcc gtaattgatc cggcggtaag
    46021 ttaccaggag atgataggct tcggagggtc acttacatgg tattccgaga gagtcacctc
    46081 aagccctcag aaggagcaga tatgcaagct actcttcgag gacctcggaa ctgatataat
    46141 caggtttaag aacaattact atcctgcagg ataccctggc gtcaaaagcc ctgccaccat
    46201 ggagaacagt tctataaaga cgctgtggaa tgtaaccggt gatttgtatg cgatggctaa
    46261 acagttcaat ccggggatac agatactggt cagctcctgg acccctcctt ctgcgctgaa
    46321 aagcaacaac aacctgcgcg aaggtaccct gaaaaggaat aacggggtgt ttatgtacga
    46381 ggccttcggt gactattgga atgaccttct ggataacctg cagttcaatc ctgactacat
    46441 aagcattcag aatgagccgg gatggattac ccccgactgg gagacctgcg agtggaggcc
    46501 ggcggaaaca gcggaattcc ctggttataa cgctgccttt gacgcagtat acaacaagat
    46561 cagcactcga tcgaacccac cgatgatgat cggacccgag gcagaaaaca taggttacag
    46621 cggtaagctg ggtggcaaca cattcgccgt tttctcagac ccgatcaaga acaaggaatc
    46681 ccttgcagcc tatgcctatc acacctacaa tttcccctcc tcggcgctga ttgcccagac
    46741 aaagtcggat ctcaacatga tacgtgatac atatggcaac aaaccctgca tcatgaccga
    46801 atactccaac ttcacctggc tcaacactgc atggttcatc atccagaaca taaacgaagc
    46861 caatgctgca gggtatatct actggttgat ggcatgggct gactccaaca atgactcaat
    46921 gatcaagctg agcccatcag gagcctatac cctgaccccg ttttactatg tgatgaagca
    46981 cttctcaaag gaagttgata agggctatgt caggattaaa gtattgtcac ccctgatgcc
    47041 gatgtgtgga ttcattgatc cgactggcaa aaaaatcacg gttgttgcgg ttaatccgaa
    47101 ccttgaatct gcaatttacg atttcgatgt gacaggtaag attgtcaagt cagtacgggc
    47161 tatgcagaca gatgctgttg tgtcttataa aacgattcag ccttccggcc ccggacagcg
    47221 ctttgttctg aagcctcaat cggtgacaac tgttgtgatt gagctgcaat aaactgatta
    47281 aaacaatccg tcaatgaaaa aagccgttgc tgccagactc ggtgatttac aggccatttt
    47341 caaatcggtt ctgccatccc ttttcctcat ctgcctgcca ggatttagcc agggagagac
    47401 cactgtgatt atctcaacgg aaaagagcga cggttctttc gtccttgctt ccggaggccg
    47461 gtgtggctca ctcttcatca gccccgatga gtggccgggg gtgacgagag cctttaccga
    47521 cctgcagaat gaccttggcc atattacagg cgtaataccg gatcttatca ccgcctcaaa
    47581 accccccagg gctgaattca tgataatagc agggacgata ggtaaaagca ccacgataga
    47641 ccgcctgatc aaaaggaaaa agattgatgc tgacgccata aatggcaagt gggagtcttt
    47701 tgttatccag actgttgaaa agccctttcc gggagtgaag agtgcgcttg ttattgcagg
    47761 cagtgacaag cggggcacca tctacggaat atacgaaatc tcgcgacagg cagggatgtc
    47821 accatggcac tggtgggctg atgtaccggt acgcaaaagc agtgaactat atgtaaaccc
    47881 tggtcgtcat gaatggggcg aaccctctgt cagataccgc gggatattcc tgaatgatga
    47941 gtacccggcc ctgacaagat gggtagctta taaatatggc gaggtaaccc cctctgccaa
    48001 cccaccgata ccccccggaa tagccaacta tggcagtgag ttctattcaa ggattttcga
    48061 ggtgcttctc cggttgaagg caaactacct ctggccggca atgtggaaca acgcattcaa
    48121 tgaagatgac cttcggaatg ctgccctggc tgacgaatac ggtatcgtca tgggcaccag
    48181 ccaccaggaa ccgatgatca gggcacagaa ggaatgggac cgcaggtatc agaaaaccct
    48241 gggaagctgg agctggacac accatgaaga caccctgttg aaattctggc gtgacggcat
    48301 acgtcggaac agggattttg aaagcatcat taccattgga ctgcgtgggg ctgatgacac
    48361 cgagatggga ccgggcgggc cggcagcaaa tatcgccagg cttgaaaaaa tagtaaaggt
    48421 gcagcgtgag atgatagcgg aggagataga taatgatgtg acgaaggtac cacaatcgtg
    48481 gtgtctctat aaggaggtac aggattatta ctatgagggg atgagggtcc ctgacgacgt
    48541 gaccctgcta tgggctgagg acaactgggg caatgtacgc cgcctgccaa cggccgatga
    48601 gagaaaacgc cccggcggag caggagtcta ctatcatttt gattaccatg gcggcccccg
    48661 cagctaccag tggattaaca ccagtcctat cccgaagatc tgggaccaga tgtcgctggc
    48721 aaagcagtat ggcgccgaca ggatatggat tgtcaatatg ggacacttca ggggatatga
    48781 aatcccgatg gaatacttcc ttgacctggc ctgggatgcc ggttcgctgg acagtgatga
    48841 catgtcggcc tggactcagc agtgggttga gggaattttt ggaccggaac atgccgggga
    48901 gatatcggaa ataatcaggg aatacacccg gttcaatggg cggcggaagc ctgaactgct
    48961 ctcgccgttt acatacaacc ttgtgaatta cgccgaggct gaccggattg tggaggaata
    49021 caatattctg gcagagaggg cggaaaagat agggcacttg ttgcccgcag agatgaagga
    49081 cccgtacttt catcttgtcc tcttccccgt cagggcttgt gcccttgtta acgagctata
    49141 tgtcactgca gggaagaatg ccctgtatgc ttcacaggga agggcaaatg ccgggaagat
    49201 ggctgaccgc tcagagcagc tctttgctgc cgatactgcc ctgatgggat actataaccg
    49261 ggtttatgct ggcggcaggt ggaaacactt catggaccag tcccacctgg gatatatcgc
    49321 ctggaatgat ccccctgaga acagcctcag gcacataaag cttgaacgcc cagccccgtc
    49381 cttaggagca atacctgccg tggcggtgga gggctctgca gaagcatggc cgggaccagt
    49441 gacgccccct ctcctgcccc cgtttgatgt tttcaacaaa cagtcaaggt attttgaggt
    49501 cttcaacagg ggcagccatc cgttcgaata caccgcttcg gctgatcacc cctggatcat
    49561 cctcagcagc agccatggca tagtcactga gcaggaaaga gttaccgtct ccatcgactg
    49621 gccggcagtt cctcacgggc accacaacgg aacagtaacc ctcactgctg aaggaagtga
    49681 gatgaagata gccattacgg ccttccggcc agaggatccc gatcctgaga ctgttgaggg
    49741 attcgttgaa gctgacggct acgtatcaat ggaagctgca agctataccg gcagacatga
    49801 caccggcgac cggcgctggg agtggattga ggattatggc agaacacact ctggcatgag
    49861 gacctcagcc cttacggatg ctccccctgc agtgccgggg aaagactccc ccgtgctgga
    49921 atacaaaata tatttcttct ctgctggtga atttgaaaca gtgctacaca tggctccctc
    49981 actcaacttc ctgcctgaaa ggaacttcag gatagggctg tcagttgatc acagcgaccc
    50041 ccagctaatc accgttgttc ccgcgggatt taacgccgaa aatttcaaca gggaatggga
    50101 agagatagtc agcaacagca cacgctatgt aacaggcaaa atcaaagtag ccggaccagg
    50161 ctcccatacc ctgaaggtgt ggatgattga tccggggatg gtgctggaaa agattgtggt
    50221 caacaccggc ggtctgcggc cgtcatacct gggcccgccg gaaagccaca ggaattgacg
    50281 atttgagatt tgagatttgc gattttgtat tgtatatcat tagcatagaa gaggaaagac
    50341 ataatcgccc gtaaggcatt aagcattgta gacaagacaa tattaccaat aattgcccgc
    50401 agggcataaa gcattgtata acccgtttac cctcacatcc cataccatga aaacaaaatc
    50461 ttatctcttt ttagcgacac tgtttgtaat tctcttaacc tttccgctga ccgcccaggt
    50521 cagacttccg cgtctgatca gcgacggcat ggtgttgcag cgcggtactg aactgaaaat
    50581 atggggctgg gcagcacccg gagaaaatgt gaatgtcgaa tttgccggaa aacaatacag
    50641 cactataact gatgattccg gtaactggat gatcatgctg ccgccaatgg aggccggcgg
    50701 gccatatata atgacactga ccgcaagcaa caaactgact gtcagcgata ttctgattgg
    50761 cgacgtctgg ctctgctccg ggcagtcgaa catggaactg ccggtcaggc gcgtcaggcc
    50821 actttacgag gccgagattg cagcagcaga gaataactta atcaggtcgt ttacagtgcc
    50881 aaagacattt gttttcacag gaccggagag tgatctgccg ggaggcaaat ggacagctgc
    50941 caacccggag acggtccttg atttttcagc tgcagcatgg ttctttgcca gggagataaa
    51001 tcaaacctac agggtgccgg tcggaattct gttaacggcc ttcgggggtt cgcctgcaga
    51061 agcatgggta agcgaggaga gccttaaggc atttcctgaa tactatgatg aactcaggaa
    51121 gctgaacgaa gactcctaca taaacaatat tgaaaaggaa gaccgccagc ggattgctga
    51181 ctggtacagc aatcttcaga agacagattt agcatacaag acagaggatt tgagatggaa
    51241 cgacattgac ctgaacactg atgaatggag cagttatact gttccgggat acttttcggc
    51301 aacacctctg aaagggatca
//
LOCUS       DAOPXU010000005        51287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_517_length_51287_cov_11.512922, whole genome shotgun sequence.
ACCESSION   DAOPXU010000005 DAOPXU010000000
VERSION     DAOPXU010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 51287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51287
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_517_length_51287_cov_11.512922"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..673)
                     /locus_tag="PKK03_00975"
     CDS             complement(122..673)
                     /locus_tag="PKK03_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNX82991.1"
                     /translation="MTEEIKKLWPELEWINDPDLREKTARTWEVALERSVLTAGDLNR
                     IPFTLLCGPDLKVTFMDHKRCVVHVARDAGMKMNEFFRNDLPVNMDVLISGAILADVG
                     KMLEYELDASGKAVQGKYGQYLRHPFSGVSLAEECGVPPEVCHIIAAHAHEGDLVKRT
                     TEAYIVHHADFMTFLPFKSRLIV"
     gene            complement(680..1876)
                     /locus_tag="PKK03_00980"
     CDS             complement(680..1876)
                     /locus_tag="PKK03_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNX82992.1"
                     /translation="MAKRTIVALPGDGIGAVVLKQAVRVLDAAGFDADYVYGDIGWDF
                     WCREGNPLPRRTIDLIAEHKIALFGAITSKPKDDAAAELSPALQGKGFTYSSPIVGLR
                     QHFGLDICVRPCRSYKGNPLNFIRRDKTGNPEEPMVDVVIFRQNTEGLYGGVEWSNPP
                     QQVYDALMTHPKFRENFGWCPKEELAVSTRIFTKKYTERIVRAAFEHAVKFGYKSVTV
                     CEKPNVIRETSGLMWKIAKEMQKNEFPGILLWNTNIDAQMMWLTKNPEDYGVIVAGNM
                     FGDIVSDAFAGLIGGLGFACSAQFSEDGIGVFEPTHGSAPKYAGYEVPIVNPVAMIES
                     ACMMLDYIDEKETATRIRRAIAEVIEEGKVKTYDMMKMTGKPEVVTMGAASTIQMTDA
                     IIARLK"
     gene            2033..3493
                     /locus_tag="PKK03_00985"
     CDS             2033..3493
                     /locus_tag="PKK03_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HNX82993.1"
                     /translation="MGELGEMKPRKLWGYFEEICKIPRLSKNEGRIRAYLLEFAKQHN
                     LEAKEDHVGNILIVREAAPGKEKVMTVVLQSHLDMVGEKNASHPHNWAEDPILPVIDG
                     RWVTASGTTLGADDGIGIAAQMAVLTDPYIQCGRIECLFTVDEESGMTGAINLKPDFF
                     TGRILLNLDSEDEGILFIGCAGGIDTQALLPYTPERITSGHRAMNISVTGLHGGHSGD
                     EIHKGYGNSIKIISRLLQDLEKKYDIRISNFSGGNLRNAIPREAFVTVTFNPTHEENI
                     LADTAAYHTLVRDEYNGLEPDLKVAATPADIPELVIDGGTQHRLLGALSIVPHGVLGW
                     SKEIPDLVETSSNLATIRLTDDNMIHVVTTQRSSSEEAKHYAAMKAETSFDLAGAKVT
                     HSDGYPGWQPNMNSAILRLMIDSYRKLFGKVPQVKAIHAGLECGLILEKYKGIDMISF
                     GPTIRGAHTPEERIEIETVQMFWDLLIDVLRHIPEE"
     gene            complement(3496..3657)
                     /locus_tag="PKK03_00990"
     CDS             complement(3496..3657)
                     /locus_tag="PKK03_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNX82994.1"
                     /translation="MNKYRCPVCGYIYDPEVGDPHSDVEPGTPFEDLPSGWACPVCGA
                     PHDEFEEVS"
     gene            complement(3674..5224)
                     /gene="citF"
                     /locus_tag="PKK03_00995"
     CDS             complement(3674..5224)
                     /gene="citF"
                     /locus_tag="PKK03_00995"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HNX82995.1"
                     /translation="MKLVENAAGRQVPDEINGKEAIPYRGVGKYRPSGRKYAPPIATC
                     IDYPEDNNKVVGSLKEALVRCGLKDGMTVSTHHHLRDGDLVSNQIFTIASEMGVKDLV
                     WLPSASFPCNDPVIQYLEDGTVNRIEGSMNGPLGMFVSEGKMKGMAVLRSHGGRVQAI
                     QDGDVKIDIAILAAPAADSFGNARGTGGPSATGVIGYAKPDYLYADKVIVVTDNLVDL
                     PCFPMEIEGNYVDYVVVVDKIGIPEKIVSGTTQITKSPDRLLIAELTTSFLEKSGLLR
                     NGATMQAGAGGTSLAIAVYVHQLLKKKGWKLSFGFGGSTKYMVDMLEEGQMGYILDAQ
                     AFDLDSVKSIEKNLNHIPYPVFNAYNYHSKGNLTTMMDIMILGATEIDTGFNGNVVTH
                     SDGLLLHGIGGWQNCLHARNVIIPVPLFRDRIPVIVDRVTTLCGPGELIDVIITERGI
                     AINPRRQDLIDSVRGKGLPLVTIEELKDIAEKMCGKPEKAAFDDRVVAAIKWVDGTVI
                     DAVRKVKR"
     gene            complement(5238..6458)
                     /locus_tag="PKK03_01000"
     CDS             complement(5238..6458)
                     /locus_tag="PKK03_01000"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNX82996.1"
                     /translation="MKQAITGNAGPKVRSDIEVTLELTETGGIELNLRSKVKTMYGKA
                     IEEQCRSLLQYFGIENARLSVNDSGALPFVIAARIEAAVKALTGTAKSFIPEMAAENL
                     YASSRDRFRFSRLYLPGNSPGMFLNAGLHSPDGVILDLEDSVAPERKDEARILVRNAL
                     RVVNFYGAERMVRINQGERGLDDLEMLIPQNVHLVLLPKCENAETVRAVDNRIREIKE
                     REGQNEMVFLMPIIESAAGVERAMEIATAAESVVALAIGLEDYTADLGVQRTKEGKES
                     LYARNRLVVASKAAGIQPIDSVFSDVGDMEGLLQNVLSAKAMGFEGMGCIHPRQIAVI
                     REGFSPSPEEMEKAKKIVIAYRDALEKGLGVVALGTKMIDPPVVARAEKTITLAVRLG
                     LLPENWMDLEKSKN"
     gene            complement(6500..7738)
                     /locus_tag="PKK03_01005"
     CDS             complement(6500..7738)
                     /locus_tag="PKK03_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNX82997.1"
                     /translation="MVTTLQDQEQETREDTEHQFVQGLKQFWAFTGFRNATRGNLIMM
                     VIGLIFIYLAIKYEYEPLLLIPIGTGILIGNIPFFTDGGTNLQVGIYQQGSVLNYLYF
                     GVLKGVYPPLIFLGIGAMTDFSSLISNPRLLLLGAAAQVGIFITFIGALLLGFAKPEA
                     GAIGIIGGADGPTAIFLSSRLANGVNVLSDGTTVKNLIGPIAIAAYSYMALVPVIQPP
                     IMRLLTTKKERLIRMKPPRAVTKTEKILFPIFGLLLTCFISPSALPLLGMLFFGNLLK
                     ESGVTKRLAETARTSLIDIVTIMIGLTVGASTQADVFLTKESVLIFGLGAAAFAVATF
                     SGVGFAKIMNLFLPEGDKINPLIGNSGVSAVPDSARVSEMEGLKADKTNHLLMHAMGP
                     NVAGVIGSAIAAGILLSYLG"
     gene            complement(7864..8292)
                     /locus_tag="PKK03_01010"
     CDS             complement(7864..8292)
                     /locus_tag="PKK03_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNX82998.1"
                     /translation="MKNFKFRINGNQYDVEVLEIEGSMAKIEVNGTVYDVEMQREIPK
                     AKTIVTSAPAHARPVKETKPAPESASGKATEIKAPLPGVIIQVLVRPGDDVKPGQTVC
                     TLETMKMENAIKAETGGKVTALNVSPGQSVLQDEVLMVIG"
     gene            complement(8316..8681)
                     /locus_tag="PKK03_01015"
     CDS             complement(8316..8681)
                     /locus_tag="PKK03_01015"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HNX82999.1"
                     /translation="MNWIYLTEGAQALDQLTWTVVVVGIGIVFLSLLIVYWFFRYILT
                     FILNFRLRNFARKKGMDPAEVQTVKTLQSGEVNAAIAMAIYQYFNELHDVESGVVTIK
                     RISRHYSPWSSKLYNMKNL"
     gene            complement(8694..10253)
                     /locus_tag="PKK03_01020"
     CDS             complement(8694..10253)
                     /locus_tag="PKK03_01020"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNX83000.1"
                     /translation="MKSQDRIKQLLDYREKARKGGGEKRIEDQHSKGKLTARERITLL
                     LDEGSFEEFDMFVTHRTEDFGLADKKFLSDGVITGHGTIDGRLVYVFAQDFTVFGGSL
                     SETFAQKICKIMDKAMKAGAPVIGINDSGGARIQEGVRSLAGYAEIFERNILASGVIP
                     QISAIFGPCAGGAVYSPALTDYIIMSRKSSYMFVTGPKVAKAVTGEDITIDDLGGAGV
                     HTSKSGVAHFAVDEEKEGLDLIRKLLSFMPQNNLEEPPQLDCDDPIDRLEDELNDIIP
                     ESPNQPYDVKDVIVRVVDYHDFVEVHEAYAKNIVVGYARFDGMPVGIVANQPAYLAGV
                     LDIEASRKAARFIRFCDAFNIPIVTFVDVPGFLPGSTQEYGGIIIHGAKLMYAYGEAN
                     VPKVTVTLRKSYGGAHCVMSSKQLKGDINYAWPTAEIAVMGPQGAIEVLEGKTISEIA
                     DAAERKEYVAKKEQEYRDKFANPYEAARYGYLDDVIEPRNTRFRVIRALRTLSTKKDP
                     GPMKKHGNIPL"
     gene            complement(10263..10685)
                     /gene="mce"
                     /locus_tag="PKK03_01025"
     CDS             complement(10263..10685)
                     /gene="mce"
                     /locus_tag="PKK03_01025"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNX83001.1"
                     /translation="MKPTHIEHIGIAVNSIADALPFYEKVLGLKCYNIEEVADQKVKT
                     AFFLIGQTKIELLESTDPEGPVGKFIEKRGEGIHHIAFAVDQIEDKLREAEAAGIRLI
                     DSTPRKGAEGLSIAFLHPKSTFGVLTELCEDKNSKNNK"
     gene            complement(10778..12340)
                     /locus_tag="PKK03_01030"
     CDS             complement(10778..12340)
                     /locus_tag="PKK03_01030"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX83002.1"
                     /translation="MKSSALKHIRTVFAVLVLLLFVFLFVDFRGVLGDKAFGPILYLQ
                     FVPSFLKFVIAGVVTSTGFIIVLILTLLTGRTYCSFLCPLGILQDTFSRAGGLVKRRF
                     RKYGYKKPYTILRYSILVITAALLAIGGVYVLTILDPYSTFGRLMTYFVQPVVLVINN
                     LLAGILGKFDIYSVYRVDIKPYELAAYIIPAAFLLLTGLLAFRYGRLYCNTVCPVGTL
                     LGLLSKVSLFRIRFDEDKCTRCGRCAMACKSSCIDFLNKSVDLSRCVNCFNCIDACKE
                     NGMSYGLVRLKRQQMNPDETDASRRGFIAGSLVLLAGGSQLLKAQGTTPSPTKDSTVR
                     EDRLYPVSPPGSSGIEMFTSRCTACTLCVNACPTNVLQPSTNQYGFAGIMQPRMDFHS
                     GFCNYDCTRCTDVCPTGAIMPLMPEAKKLTQIGKAVFIKENCIVNTEKTACGACSEHC
                     PTKACHMVPFEGRLLIPEVKDEICIGCGACEYACPTRPFRAIFVDGNPIHEAASKPET
                     EELKEVPSEFPF"
     gene            complement(12347..13273)
                     /locus_tag="PKK03_01035"
     CDS             complement(12347..13273)
                     /locus_tag="PKK03_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNX83003.1"
                     /translation="MKRRNFLRSSLGAGLAAGAFMSFGGYEKAFAGKRLPSGADYDLI
                     AVMGGEPAAMFDMGIQALGGMGTFVKKGQKVLVKPNIGWDVVPEKGGNTNPALVKRII
                     EHCYRAGAKEVFVFDNTCDNWIKCYKNSAIEQAVKDAGAKMVPGNSESYYQEVTIPNG
                     KKLTKAKVHELVLSSDVFINVPILKHHSSAMMTGALKNMMGVVWDRGYWHRNHLNQCI
                     ADYALYSKKPNLHVMDAYNVMMRNGPRGVSVADLSNMKSLMLSTDWVAIDAAGAKLMG
                     KEPSSVEHIALAAGMGIGKMDLATMNIHRMKM"
     gene            13427..14722
                     /gene="larC"
                     /locus_tag="PKK03_01040"
     CDS             13427..14722
                     /gene="larC"
                     /locus_tag="PKK03_01040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HNX83004.1"
                     /translation="MKILYYDCFSGISGDMNLGALLDLGVSSEILTDGLQKLNTGGWK
                     LDISRDQRHGITGTKVTVITDHNDHEHTYGNAHDHDHHHDHEFNHGHNHKHDHQHNHE
                     HRYDHQHEHRNLADIEKIIKGSGLPAPVSDLAMKIFRHIAEAEAAVHDKPLHEIHFHE
                     VGAIDSIVDIVGAAICLNELGADRVYVSEIELGSGMVRCQHGLLPVPAPATARIISGF
                     PVHIGGVDFEATTPTGAAIIAAIAEPLPPSMRMVIRKSGYGIGQKNNPSRPNILRVSL
                     AETAGEAEKGHSARLVECNMDDMNPEMSEYISGRLFAAGAGDVWFTPVIMKKGRPAFT
                     LSVICEEDQLNAVREILFTESTTIGLRVLSFSKETLHREFEEVETRFGKVTVKKSYFN
                     GRLVSVKPEADRCAAIAATMGIPMKNVIREITSLTREES"
     gene            14719..15522
                     /gene="larE"
                     /locus_tag="PKK03_01045"
     CDS             14719..15522
                     /gene="larE"
                     /locus_tag="PKK03_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HNX83005.1"
                     /translation="MTVALKLDRLKSILSESGSAVIALSGGTDSTFLASVAAEVPGLR
                     MMAVTVSTPYMFSSEVDEASRFCSEKGIAHRQAGFDIPPSVALNPPDRCYLCKKEVMK
                     AISSVAKEEGYAFIFDGTNADDLLDYRPGLRALAEEGIRSPLAEARLTKDEIRALARV
                     AGLEVADRPSNTCLLTRFPHDTEIKPADLRKAGEAERILKEAGFSGARLRVHGELVRL
                     ELMREQFELIALSETRMAIAAALKKLGYRYITIDAEGYRSGSMNKTTKE"
     gene            15519..16265
                     /gene="larB"
                     /locus_tag="PKK03_01050"
     CDS             15519..16265
                     /gene="larB"
                     /locus_tag="PKK03_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HNX83006.1"
                     /translation="MNSSELEKILKEIRTGKVSVEEAMEKLRDFPYTDLGFARIDHHR
                     ELRTGYPEIVYCAGKTPEQVRAIFKAMEGHDNNIIGTRAVKEMFNYISPFFPEAVYYE
                     TARIISIRRKEIVPPVSKIAIITAGTSDIPVAEEAAVTAELLGNEVVRIYDAGVAGIH
                     RLVDKLPEIRNCRVSVVIAGMEGALASVVGGLVNMPVIAVPTSVGYGASFEGISALLA
                     MMTSCAAGVTVVNIDNGFGAGFAASRINRL"
     gene            16271..16912
                     /locus_tag="PKK03_01055"
     CDS             16271..16912
                     /locus_tag="PKK03_01055"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HNX83007.1"
                     /translation="MADELLLSTAYFPPAGYMSLIACSMGVLIEREENYHKQTWRNRC
                     LVMSANGPLPLVVPVLRGSFHKTAIRDLRIDNSRRWKELHLRGIVSGYAAAPFFEFYF
                     DLISAVLSKTHPFLLDLNSEALEAVCRATGIDVPVGYTDRYEPEGTRENDYRYRITPK
                     KPSEIPGYRDLPYTQVFGEKQGFVPGLSIIDMLLNNGPGTRALLLRSLGADNC"
     gene            complement(16918..18432)
                     /locus_tag="PKK03_01060"
     CDS             complement(16918..18432)
                     /locus_tag="PKK03_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83008.1"
                     /translation="MKRKIFLIAASILMLPAWLAGQNMDDALRYSRLFYQGTARFNGM
                     SGAFTALGGDVSAIGLNPAAAGVFRSTEVSITPAVFFRDLNTDWNGYGSDDSYSGLTL
                     GQAGLVSSFRTGSGKGLTNLNLAYTFNRTNNYYRNAVIDGISNNSSMADFWALQATGY
                     RTGELGGTAFMAYDTYLIDTLPNYLDEYASIFSYYGETYPEYGQKLKRVIDNSGYSNE
                     HTIALGASLGDKVYLGAGFGITNVSYTGHYSHLESDDAQNTFDFVNFTYTDHFKATGS
                     GWNFKLGAIVRPFESLRIGVGFTTPTIYTIDEVFYNSLSVKLDNDTPADPSDDANPVV
                     NNEDMTYRYRVTTPYHLNAGIAYQIGNFGLISADYEFIDYATARLSKGADGYNFETEN
                     EDLAAEVGATGNLRLGAEARLGPLYLRGGYSHHGSAFTEGTLNEDASSNGYSAGLGYR
                     QDNFYIDVAMVWLNSYESYMMYPDDSRAVPEYTSDPVNLNIKDKYLSVTLGLKF"
     gene            complement(18513..20000)
                     /locus_tag="PKK03_01065"
     CDS             complement(18513..20000)
                     /locus_tag="PKK03_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83009.1"
                     /translation="MKRFWYIIPAILVITSACSSLRFTGANNDDLYYRPSENPVAVAG
                     PTVQDRNAGQSYYDNIYAGDTLIADEFVPDDEYSAYAAAGEPTIVNNYYGGAADRIYL
                     FNDDYFYPYWRDPFYYSPFSMRLGFGYGYWGSYGSPYSWGYDPFYYDYYSPYSYYSPY
                     YSWGYSPYSYYNYYGGYYGYGYPGYYPWYSGYGYPSHYNDGGYVENIARRGGYSSMSR
                     GSYSNTDIRTKSGYAPSGDMNSRRDVSGTALPAGTGNTPAGSRRTATESSPNREAAAA
                     GTTTGTRRTQDGTVSNSNQGAATVSRPEYTRRESDNTTVQNQTQTRTQSPVRTETTTQ
                     TRTQQQTPVRTQTQTQTVNRPQYQPAERSYTPSYNNPRVSSRPSYNTTRSNSGTTTQS
                     SGTVYNRTSTPRSSSTTPAYTPSRSSSSPVQYSAPSRSSSGSSYSSGSRSSGSSYSSG
                     SRSYSSGSSSSSGGSYSSGSSSSSSSSSSSSSSSSGGRSSSGGRR"
     gene            20200..21675
                     /gene="proS"
                     /locus_tag="PKK03_01070"
     CDS             20200..21675
                     /gene="proS"
                     /locus_tag="PKK03_01070"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNX83010.1"
                     /translation="MAKFLTNREENYSQWYNDLVIKADMAENSAVRGCMVIKPYGYAI
                     WEKIQSELDRMFKATGHVNAYFPLFIPKSFFSREAAHVEGFAKECAVVTHYRLKNSPD
                     GKGIVVDPDARLEEELIVRPTSETIIWNTYKDWIQSYRDLPLLINQWANVVRWEMRTR
                     LFLRTTEFLWQEGHTAHATRQEAEEEAVRILNLYTDFVENYMGVPVIKGVKTESEKFA
                     GAIDTYTIEMLMQDGKALQGGTSHFLGQNFAKAFDVQFTNQEGKLDYVWATSWGVTTR
                     LIGALIMAHADNNGLVLPPKLAPIQVVIVPIYKGNDQLDKISDIAVDIRQQLEKAGLT
                     VKYDNRDTQKPGFKFAEYELKGVPVRLAIGPRDAEEGTVEVARRDTLTKETIRRENVV
                     AHVIGLMEDIQQNIFRKAVEFRENNTFTADTWEEFTDIIENRGGFVLAHWDGTPETEE
                     KIKDLTKATIRAIPLDSREEKGKCILSGNPSNQRVLFARAY"
     gene            21809..22522
                     /locus_tag="PKK03_01075"
     CDS             21809..22522
                     /locus_tag="PKK03_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83011.1"
                     /translation="MADEITQSKILIVDTLKGKSVLKQKVFDNTAVAFMMVKDILKNL
                     ARDVNGNLGGVDQRIRMEYTDRSTFDAQLKVAADLLLFSMHSNIFQFDREHPAWKTAY
                     IQKNKFNAYSGIINIYNFLADSFKYSRLDDLGYLIARIFINHENQYFVEGKRQMGMLF
                     TNYGNEPFSQESLKKIVYTAINYSLEFDLLVPPYDTVKIASVGQAESKIQHSRLITGK
                     RLGFQFNSDDILDDNSKPK"
     gene            22519..24342
                     /locus_tag="PKK03_01080"
     CDS             22519..24342
                     /locus_tag="PKK03_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83012.1"
                     /translation="MNPFTNRTFKTLFFPFLTLFIILHGLPVAAQGTSAPDTLSRAFY
                     DSLKVRAEKRRLTSLLYDMIIVTPAPPGQAREKLASTTAYEAYEGRKIRNIEIIRLDA
                     FGQNIDNPEGKTPSRAERLMNSTYTKTRRFVLIRNLVFKTGDTISSLKLSDNERLLRE
                     LPFIDDSRITVVPADSNYADIAVVVREKYPVGANLRIDDIRSGRVGVFTRNFVGLGHE
                     LEISAPYDYNEYDAPGIGLRYSINNIARTFSRLNMQFSDGLGTTHMGGTFSRDFVTSE
                     TRYAWSASVIFTRTNDEIDTMTVPAPLRFTRQDYRAARSFMLDRNTVTRLIVAGRYMH
                     NNVFRRPSIDEFSYYRFQNYQSVTGSLAVSSQRFLNTSLIYSYGRTEDIPYGYMAELF
                     LGREKNEFKWRNYAGGTVAYGNFFTGVGYLYAGAGISAFYNNGHTEQGMFQGSIRYFT
                     PLARVGQSRVRTFINLNYARGFNRYSDEFLYFRDDGLIRGFRNDSLGGNTRITVSVEP
                     VLFIHKPVIGFRFAVFAFADAGLLSRGRQMGADYYTVAALGAGVRIRNDQLVLNTLQV
                     RIAWYPNSPPWSVSSLVTANSIMRLRPPGFEPGPPGLVPYL"
     gene            24441..25757
                     /gene="tilS"
                     /locus_tag="PKK03_01085"
     CDS             24441..25757
                     /gene="tilS"
                     /locus_tag="PKK03_01085"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNX83013.1"
                     /translation="MLSRFLEFSEREKLFTRGSRLLIAVSGGIDSMVMAWLFREAGIE
                     YSIAHCNFSLRGDESDGDEEFVAAYARTSSISFFSRRFDTMTYAGTHRISVQMAAREL
                     RYDWFRSLIRSEGFDSVAVAHNLNDNVETFLINLSRGTGLHGLTGMSPRTGDIIRPLL
                     FATREEIASYASARGIDFREDRSNAEVKYIRNRIRHKVIPELEKVNPGILAAVADTMK
                     HLSSDSSLVDTYLSQLSGQLFTFSGDSAGVEISSLMALNPLEPHIFGLFRPFGLFPKQ
                     TDEVISLLQSGTGRSVYTATHRLLNDRGRIIISPRISEAPEEYTFNSIDEIRISGLFS
                     ALRITEPSDDALPATHLTASLDLDLVAFPVTVRPWKPGDRFMPLGMKQMKKISDFLID
                     LKVPVTEKEQVLLLLSGSDVMWVMGYRIDDRFRVSEETGKIIVLTL"
     gene            complement(25789..26784)
                     /locus_tag="PKK03_01090"
     CDS             complement(25789..26784)
                     /locus_tag="PKK03_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNX83014.1"
                     /translation="MNTIGTIFRVSLFGESHGPAVGVVIDGCPAGLPFWREELMADLS
                     RRKGLLPGTTTRRETDEPEVISGIFNDCTTGAPLVICTRNNDFRPGDYEQFTSIPRPG
                     HADFTAGFKYKGFSDMRGGGHFSGRLTWGIVAAGYFARKILSPAIISATLVEAGGEKD
                     ITSAIARAMETNDTVGGVVECVVKNVPKGIGEPSFMSVEAALGMIAFGIPAVNGVEFG
                     AGFASSRSYGSLHNDPFIDARGKTSTNNAGGINGGITNGNDLIIRVSVKPAASTGVPQ
                     KTFDTATGEMTDLEITGRHDACIARRIPVILESAVAIGLADLLMIDRGINGMRDR"
     gene            complement(26799..27845)
                     /gene="aroB"
                     /locus_tag="PKK03_01095"
     CDS             complement(26799..27845)
                     /gene="aroB"
                     /locus_tag="PKK03_01095"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNX83015.1"
                     /translation="MNRIRINSPGGESLIILGGTVDDVVTLLPQRGLFIITDRNVQKL
                     YGKKFPPGEVIAIEPGEKSKTLRMVEEICSKMLEAGADRSSFILGFGGGVVCDIAGMV
                     ASVYMRGVRHAFVSTTLLSQVDASIGGKTGVNLGEYKNTIGTFRQPEFVLCDHAMLTT
                     LPEAEVRSGMGELFKHAVIRDRNLFFDISASAGHIIAADTAVTGDLILRAVRVKAGVV
                     RHDPLEKGQRRILNFGHTFGHVIETHSGIPHGVAVAKGMAIAAELSVWKGEMAHSELR
                     LLQTALEKAGLMTCHKLPPDVVSMISRDKKSESGRVNFVLLKSVGRTVIRKISTTELQ
                     AFVNYYNEKESQSQ"
     gene            27963..28607
                     /gene="nth"
                     /locus_tag="PKK03_01100"
     CDS             27963..28607
                     /gene="nth"
                     /locus_tag="PKK03_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNX83016.1"
                     /translation="MRIAERYRGIMEWFEANMPLPETELEYSDPFQLIVAVILSAQCT
                     DRRVNMITPALFARYPDASSMARATQEEIFGLIRSCSYPNNKAKHLRGMAEMIVSRFD
                     GKVPSTREELTMLPGVGRKTANVIASVVWNSPVIAVDTHVFRVARRLGLTPGARNPLD
                     VELRLTREIPEESRPKAHHWLLLHGRYTCTARKPGCQECGLKQWCAWYASHSGK"
     gene            28703..31888
                     /locus_tag="PKK03_01105"
     CDS             28703..31888
                     /locus_tag="PKK03_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNX83017.1"
                     /translation="MEKRKESVNEPEHSAEYLTSLRHLAEIPELAFNSTNQLITLSNL
                     ETQKYIEVNQAFLDTTGYSKEEIIGKTSDDIQLYVDLIQSRKYMRLLSRLKKVSDLEI
                     KLRMRSGDERTFLFSARTVFLGDEIFLITYYNDISALNGGFARAESEKILREIFDSMS
                     SYAIILRREGENKHFIIDFNYRAEDVEQIDRKDVLGKEIGETPFARNETFLSLLENLE
                     SSHRPYKIPVSPDGSDDSGYYIGLLLSSGDILITWEAGKAQKERESEILKQGIVFETF
                     AELLPEMIMEIDTGGHVTFMNTQGMMTLGFDNNDLASGVTLSSLFMPDDLDRLMRELS
                     GLTGPGQITFDEYILVNKRKRNVSVLFHAGPIIIRNRVTGYRCVLTDVSKHKEYEQKL
                     SNEKALLEHLIDAAPEAIVLTDFDGRIIRINQGFTHLFGYDADEAMNRMIDDLVVPEE
                     LRDEAVKIDETALSSGTGYLETIRKDKQGNRINVSLIASSVISNDMTIAFLGIYRDIS
                     REIKSRLMQEIQYNISTVALQDSDIFDIYPTIVKEIGKLWNVNNFFIALYNREKDTLT
                     FPFFADERDHFQETAARNTLTGWVIRNNKAVLLDENDILMIEKTGAISLVGSPCKIWM
                     GVPLRMDDEVIGAICLQDYKKIDAFTNEDLQVFEFIANQIVLTLQRRRMLDNLITARK
                     RAEEAAFSKQQFMSTMSHEIRTPLNEVIGITNLLWQSDPREDQMDYINTLRFSANHLL
                     SLVNDILDYNKMEAGKIVFEKTEFDLTSMLEDIKRSYSHRALEKGIYFTLEKSPDLPV
                     TLIGDPIRLNQILSNLLSNAMKFTSQGGVTLKASVKDRQEGRAVIEFSVADTGIGIAR
                     EKQEEIFESYAQASSDTTRKYGGTGLGLAICKRLVDLQGGTIGVTSEPDNGSVFTFSI
                     EYSMPAAEPRTAERGTASDSMKALVGKKILVAEDNKINFFVANKFLESWGVKVTHVEN
                     GALALEEIRKQDFDLILMDLHMPVMDGIEATRIIRSSTDRKISQIPIVALTAAVMSEA
                     HDKIENLAINDYVLKPFKPKDLYDRILKNSR"
     gene            complement(31911..33113)
                     /locus_tag="PKK03_01110"
     CDS             complement(31911..33113)
                     /locus_tag="PKK03_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX83018.1"
                     /translation="MKKILFSLAVLLASQIMSGQTGYETLLRAITLAGRGEAGEAAAL
                     LAGTDELNGDANLLSVRGDIYLRASMIREARADFMRAENLSQGSGLYGLARCAAAGGD
                     AKTAVSLLEAHLKSALRKPEPEIMLDPSFRSVSSSPEWRALWKKDWYRGDERKSWEID
                     YYLKSGRNDMAAGAYAELASLYPEKPVTEYCHACILMSNGKYREAADILETITGNSDA
                     PAGWLYTLAEAREGEGSAYAAAAIYDRMISAHVADPALLLKRARMLIRSGDREAAKKE
                     MQRYISIDPDNTEALGLIGKTYAEEGAIYEALPYLNDNVDKHPGEASSFRLRGDAWLA
                     ARTWDRAAEDYTMSLDLDPENGNVNLNLGIALINSGRSDDACYYLRKARSLGVKEATD
                     YLARYCIR"
     gene            complement(33119..35725)
                     /locus_tag="PKK03_01115"
     CDS             complement(33119..35725)
                     /locus_tag="PKK03_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNX83019.1"
                     /translation="MRRRFFYALSLLLAVTFSATATHNRAGEITYRQISDLTFEVTVT
                     TFTYTLSKADRPALDVEWGDNSITNVARISETILPNNYKKNVYVAEHTYPGPGVYRIV
                     VQDPNRNFGVENIPNSVNVVFSISTILIVNTAVGRNSTPVLLNPPYDKAALGQIFIHN
                     PAAFDPDGDSLSYKLTVCTREDGKPIQNYVLPPASRKFYVDSISGDLVWDAPTAIGIY
                     NVAMEIQEWRAGIKIGIVVRDMQIEVYETDNKPPVTSQLNDLCVEAGQQVTLDIQATD
                     ADLDSVTLLATSGIFTSTDCPAAFTTLSRIAGHTTARLSWTPCHNAVRHQPYDIIIKA
                     EDSNRELQLVDIDNMSIKVLGPSPVLLTAVPQGKVVQLSWAGYVTDQVAGFSIYRREG
                     ASTLPADPCYDGIPPSAGFVKVGYAGGPGTVSFTDSDDALEAGTEYAYRIVAVYPNGT
                     ESKPSNEIVTSLITGIPLITNVSVRNTHAVNGSLTLAWQKPGRLDTIPANGPYEYLIY
                     RAEGIAGTSWQLIHTLPTTDLNDTVYIDTLINTLDKGWVYKIELYNNAPGNRFLIGDP
                     GIASSLFLTALPGDRKVRFEMTRNVPWINTSYDFYRYNGSTWEPVGSTNQLTFTDGGL
                     VNGTEYCYYVVSRGGYQGTGTPKNLVNLSQRACAVPVDNEPPCTPALTVSSQCDSLYN
                     TVRWTIEDPLCLDDVAGYKLYYKQTTEEDLTLLATINDREVRKYVHSLPDYVAGCYAI
                     STFDQIGNESPLSTMVCVDSCNFYEIPNVFTPNGDDFNDWLLAKTSALVEKVEFRLFN
                     RAGVLVFSTQEPRLNWDGTYKGRVVPTGVYYYDCEVFERRISGLEQFHLSGFIHVFAE
                     KDAGPGIIEGKK"
     gene            complement(35737..36198)
                     /locus_tag="PKK03_01120"
     CDS             complement(35737..36198)
                     /locus_tag="PKK03_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83020.1"
                     /translation="MKTITLTLLLLIHPVHVSLTGIDYDGVNRVWSVFVKVWSDDLEA
                     DMNLGLSNGGEITGSTDERYFMYLSDRVMILEDGKPVNMELLSAGADGLEHRFTLRAK
                     GRKRVKNVAVMNRIMTRLYEDQANMLLFGYGGTEEGYRFTVTDTLKSYLVK"
     gene            complement(36227..37684)
                     /gene="pyk"
                     /locus_tag="PKK03_01125"
     CDS             complement(36227..37684)
                     /gene="pyk"
                     /locus_tag="PKK03_01125"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008566502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNX83021.1"
                     /translation="MARSLLKKRTKIVATISDRNCEPAFLRRLHEAGMDVVRVNSAHL
                     DTDGVRKIVANVRSISDNIAVLLDTKGPEIRTTSCEEPLRFHKGDTVRVSGNSAAGTT
                     REVINVNYCDIAKYVPEGAVILIDDGELEIKITGREGKTLTGRIENDGILGSRKTVNI
                     PQVRINLPSVSERDLGFIDLAVELDIEFIAHSFVRSKEDVLAVQRLLDRRSSAIKIIS
                     KIENQQGVDNLEEIMDHSYGIMVARGDLAIEVPFERIPGIQRNIIARCIEKRKPVIVA
                     TQMLHSMIQNPRPTRAEVSDVANAVYSQTDALMLSGETAAGKYPVEAVRTMTRIASET
                     EKEKDQYIEMPAGTLSGPVSAYLAKVAVKTSIRIKARAILADTVNGTAIRNMAAFRGI
                     NPVIAHCYSHRTMRELALSYGVIPIFIEKKMSVDEFIHVSVRELLRNYTMKDSDIVVV
                     LAGNFSRGTGFSFIEVGTVDYLRERAGVTEQDLMA"
     gene            complement(37692..38120)
                     /gene="aroQ"
                     /locus_tag="PKK03_01130"
     CDS             complement(37692..38120)
                     /gene="aroQ"
                     /locus_tag="PKK03_01130"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNX83022.1"
                     /translation="MTIEIINGPNLNLLGKREPAIYGTEGFESVLNRLKERFPQVSFR
                     YSQSNIEGELVNRIQEAGSTADGIIINPGGYSHTSVAMADAVAAITIPAIEVHISNLL
                     TREEFRHTSLTGRYCRGTIMGMGTDGYRLAAEALIEILKK"
     gene            38225..39133
                     /locus_tag="PKK03_01135"
     CDS             38225..39133
                     /locus_tag="PKK03_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HNX83023.1"
                     /translation="MTISWTDAIRGFGRYLRIERSLSPNSVAAYLADVRRLHDWVESH
                     RPGTWPSALTYRDLSDFVAFIATRDDAVRTQARVISGIRSFCRYMLIENLVTDDPSAL
                     LELPRTGMKLPEVLSVEEIDRMVASIDLSVPAGHRNRAIIETMYGCGLRVSELVNMRL
                     TDIHRGEGFVTVTGKGNKQRLVPIGTVTLKEIDNYMERRMMMPVIIDINILFLNRRGR
                     KLTRVMVFNIVRDCAAAAGITKTISPHTLRHSFATHLVEGGADLRAVQEMLGHESITT
                     TEIYTHIDRSYLRDTVLMYHPRARRR"
     gene            39307..42036
                     /gene="ppdK"
                     /locus_tag="PKK03_01140"
     CDS             39307..42036
                     /gene="ppdK"
                     /locus_tag="PKK03_01140"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018027654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNX83024.1"
                     /translation="MSKVKRVYSFGNKTAEGRADMKNLLGGKGANLAEMCLLGLPVPA
                     GFTITTEVCTEYYENNTQLPAELMPEVWEAMKKTEQAMGMKYGDRENALLVSCRSGAR
                     SSMPGMMDTVLNIGLCSETIPGLIKKTGNPRFVWDSYRRLIMMYADVVMEKAEGIEPA
                     DGKGIRKQLDEMLDEFKHGRGYKSDTDLTSDELKNLAEEFKKRIKKSLGKEFPDDARE
                     QLEGGIKAVFKSWNGKKAVSYRRIEGIPDEWGTAVNVQAMVFGNMGDSSATGVAFTRN
                     PATGENLFYGEWLVNAQGEDVVAGIRTPNPLNDDTKNEQNKHLHSMQESMPGTYKELV
                     DIRNILEKSFHDMLDIEFTIQEGTLYMLQCRAGKRTGTAALNMAMDMLQEGLIDEKTA
                     VMRVDPAQLDELLHPICDPAAEKKVSPLVKGLPAGPGAAVGKIVFTAEDAVAWNRKGE
                     KVILVREETNPEDVEGMRAAEGILTARGGMTSHAALVARGWGKCCIVGAGGLHVDVAG
                     KKAKVTGSDVVLKEGDIITLNGTRGNVYTGSLKLMDASENPRFQSFMKLVDKNRTMGV
                     RTNCDNPDDAKMAREFGAEGIGLFRTEHMFYGKGSEQPLFILRKMILSENETERRQAV
                     NELFPFVKKDMKGTLEAMNGLPVTFRLLDPPLHEFVPQGHEKQAELAKALGISVEAVA
                     KRGEALHESNPMMGHRGVRLGVTYPEVSEMQIRAMFEATAELLKEGKNPLPELMVPVT
                     CDVSELDVTKKIYDRVLAEVLKKFNLSKIDNKYGTMIEIPRATLLADRMAKTAEFFSF
                     GTNDLTQMTFGFSRDDIGGFLHDYLDNKMLAADPFQTIDQDGVGQLITMACVKGRSTR
                     PNLKIGICGEQGGDPASVEFCFKNGLTYVSCSPFRVPIARLAAAQAAIRASRK"
     gene            complement(42234..44660)
                     /locus_tag="PKK03_01145"
     CDS             complement(42234..44660)
                     /locus_tag="PKK03_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNX83025.1"
                     /translation="MKPGKWCWLTAALLCLPVLLYGQRTDFSGLKIFINPGHGGHDGD
                     DRHMIATEFWESEGNLAKGLFLRDLLEARKATVYMSRTTNYTSDDLKLSVIAELANQA
                     NADIFLSIHSNGFDGTRNQPLVLFRGYDNSPVFPAAKSMAQILWEKLYEKSNYWTHSF
                     QWVKGDWTFYPEWGDQVGLGVLRDLTMPGVLSEGSYHDYIPEGWRLRNENHLRHEAWA
                     MLRALQQFYNVNPESLGIIAGTVRDELTSPPYYYGPETTDKNSPINGATVTLKPSGRS
                     VMIDNLNNGFFMFDSLPPGNYEVIASGMTDFYNDTVNAVANSGMTTLADLLPSFDTAR
                     VPVVIEHLPSTTDSLPFNQAFSFRFNVPMNMLAVQSALVFEPEVSVTYEWDEKSRILT
                     VKPKTGYASGTPYLIRLTTAATSRWNVHPAGEYLVSFVTKKRPKLVIDRIWPSQGSAG
                     ITLFPRITILFDAPLSQESATAGIRLLDSQSVPLTKQNEQFKSEGGKGSYTFEPASQL
                     ERNRIYRIEIDASVKDVSGISAGTPVSTTFTTRTEAYQSGSVIESFDNIGAFWDPETS
                     GSTVGTDNPMTTFTASYNTFKVNAPAGRLDYLFTGDNGGICRVFDTGKPLIGNNTSQV
                     FAMWVYGDLSENRLEYWFYSPGSVNQVVPAATINWAGWDLIAIPVSSIGGSGEWQYHS
                     IVVRQNSGGLKSGTMYFDDAMVFTPTGIEVIPEDLTELTVFPNPVAGEGRVSFTLRSE
                     CRVDISLIASDGSLVANIFTGTLGAGEHSIPCSPSQSVAAGVYALRLGISGGEDDAWR
                     YVSRRWVVLK"
     gene            complement(44657..45568)
                     /locus_tag="PKK03_01150"
     CDS             complement(44657..45568)
                     /locus_tag="PKK03_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83026.1"
                     /translation="MRTVILLSALFLTISICAQNIRVRSVKKVSPEESGSYLLSGVVP
                     GSGLLLVSGSGYNGLCLLDTKTGRVQVITGDTGAGYEPAVTADGRKVMYRSDSFIDNV
                     KFSSVSSYDIATGTKELLLEKGRDVLPPAVAGNNVLVRSDKGTQVEQAGPALKSGNNG
                     TFLLTEDLMPVLFRGSERKAMMPNGDGFYIWASLSPDGSKILYNYRGQSSFICDTDGR
                     ILHDLGRINAPKWFNDRLVIGMDDKDDGHRITGSELVYYSLGDKKRVILTSTPDRSEM
                     FPFAAGNRRIAFCTDNGEIYMMKVRIR"
     gene            45713..47329
                     /gene="pckA"
                     /locus_tag="PKK03_01155"
     CDS             45713..47329
                     /gene="pckA"
                     /locus_tag="PKK03_01155"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNX83027.1"
                     /translation="MAEEILKQYGITKVKGIVYNPSYERLYEDELSPGLKGFEKGVLT
                     TTGAVAVDTGIFTGRSPKDKYIVRDSTTENTVWWKSESAPVSDNKSLTNEAWDHCKKI
                     AECQLSGRELYVMDCFSGANRDTRLCVRFVMEVAWQAHFVKNMFIRPTEEELKDFKPD
                     FVVLMASKAVNPEYQKFGMNSENFVLFNLTERMALVGGTWYGGEMKKGIFSVMNYYLP
                     LKGIAAMHCSANVGKKNDVAIFFGLSGTGKTTLSTDPERALIGDDEHGWDDEGIFNFE
                     GGCYAKTINLSKENEPDIYRAIRRDALLENVVVDDKGEIDFASAAKTENTRVSYPIYH
                     IDNIVRPVSRAGHATTVIFLTADAFGVLPPVARLTEEQTQYYFLSGYTAKVAGTERGI
                     KEPMPSFSACFGAAFLLLDPIIYARELTRKMRAHNADAWLVNTGWMGGPYGTGKRIDL
                     PSTRRIIDAILNKTINDVPYTTIPVFNLSIPSSLPGMPSDLLDPGKKWPDYEAWLKAA
                     RELGEKFVKNFSKFTSNVETAKLVSSGPKP"
     gene            47340..47972
                     /gene="plsY"
                     /locus_tag="PKK03_01160"
     CDS             47340..47972
                     /gene="plsY"
                     /locus_tag="PKK03_01160"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNX83028.1"
                     /translation="MVISLSVIAVVLAYLAGSIPSAVWAGKLFHGIDVREHGSGNAGA
                     TNTVRVLGWKTGIPVLIFDLFKGWLAVMLPEFLDAAPEGSEPMMVLQIVCGMAAILGH
                     VFPLFAGFRGGKGVATTFGVLLALHPLLTLTCAGIFLLVLFISGYVSLSSMTAVAMFP
                     ILLVTVFRTPSTLLIMFSIVVAVAVIITHAKNIRRLLRGEEKKFIRKKKD"
     gene            complement(48013..48921)
                     /locus_tag="PKK03_01165"
     CDS             complement(48013..48921)
                     /locus_tag="PKK03_01165"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNX83029.1"
                     /translation="MKNIIFVLLSVILAALQSCNPKGSEYHLYYLGGQSNMDGYGYVA
                     QLPAGLTGEFEGVMIYQGNTVGDNTAVDGRGLWSTLRPGHGAGFVSDGTANTYSDRFG
                     VELTFAERMKELFPDEKIAIVKYSRGGTSIDTTAAGSFGAWLPSYTGGNGINQYDHFL
                     ATVKGAMAAGDIDGDGKKDRLIPSGILWMQGESDANNDYATSVYKENLALLVDSIRKV
                     FSAPGMPVAIGRISDSHNDADSIVWTWGDDVREAQATFVAEDGNAALVTSTDNYGYSD
                     MWHYDSEGYIDLGRKFADAIAGLRQR"
     gene            49028..49603
                     /locus_tag="PKK03_01170"
     CDS             49028..49603
                     /locus_tag="PKK03_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNX83030.1"
                     /translation="MEGIKGTRTEQNLLKAFAGESQAKNRYEFAAKVAKEEGYEQIAA
                     IFLETALQEQQHAKRFFGYLEGGMVEITAAYPAGKTLTTGDNLEAAAAGEHEEWSELY
                     PMFAKVAEEEGFKEIATCFKAISAAEKVHEERYRKLLENVMGNKVFEREEKVFWYCRK
                     CGYVHFGTKPLKNCPACLHPFNYSEVYAQNY"
     gene            49620..50171
                     /locus_tag="PKK03_01175"
     CDS             49620..50171
                     /locus_tag="PKK03_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNX83031.1"
                     /translation="MTDGEEMLSLIRGRQSERKYLDKPVEREKIERITGAGRLSPSAC
                     NGQPWHFIVVDEPALRDEVAAATESVVLRMNSFVREAPVLIVVVREKSNFSSRAGDLI
                     KQKDYSLIDIGIATASIVYQAAAEGLGTCIIGWVDDKRVRKVLGIPASRKVELVISVG
                     YTDNRLRDKSRKPPEEIITYNRY"
     gene            complement(50213..>51287)
                     /locus_tag="PKK03_01180"
     CDS             complement(50213..>51287)
                     /locus_tag="PKK03_01180"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNX83032.1"
                     /translation="PDFNTPDDIKEAAKKAIDDNYSFYPPVPGYNDLREAISRKFKEE
                     NGLDYTPDQIIVSAGGKHSLINVVLSVVDPGEEVILLAPYWVSYLDHITFAEGKPVIV
                     QTSIDSDFKVTPAQLEAALSPRTRLLIFNSPSNPTGMVYTREEMAALVKVLEKYPQVI
                     IISDEIYEHITFSGKHVSLASFSSIYDRVVTVNGVSKGYAMTGWRIGYIGAPLWLAKA
                     CNKLQGQFTSGVSSISQRAALAAVTSKSDSKERMREAFLRRRDLICRLLGEIPGLRVR
                     VPQGAFYVMPDITNFIGRSYDGRVMKNADDLTFFLLAEARVAMVSGTAFGADNCIRIS
                     YATSDDRITEAVRRIREALEKLG"
BASE COUNT        12255 a        13061 c        13463 g        12508 t
ORIGIN      
        1 acacccagac aactgacgtg gagacggaga ccaacggtct gatgacctat gaccgcaaag
       61 tggacaagat gggattcacc aatgtgagga aggcgatgga aggaaatagg cagtaggctg
      121 atcaaacgat cagcctgctt ttgaatggca ggaatgtcat gaagtctgcg tggtggacta
      181 tataggcctc ggtggtgcgt ttgaccaggt caccctcgtg ggcatgcgcc gcaatgatat
      241 ggcacacttc gggcggcacc ccgcactcct ctgccaggct gacgcctgag aacgggtgtc
      301 tcagatactg cccgtacttt ccctgcaccg cttttccgga tgcatcgagt tcatactcca
      361 gcatcttgcc gacatcagcc agtatagcgc ctgagataag cacatccatg ttgaccggca
      421 ggtcattgcg gaagaactcg ttcatcttca ttcctgcgtc gcgggcaacg tggaccacgc
      481 atctcttgtg gtccatgaag gtgactttaa ggtcaggtcc gcagagaagt gtgaatggta
      541 tgcggttcag gtctccggcg gtgagcacgc tccgctcgag ggcgacctcc cacgtgcggg
      601 cggtcttctc tctcaggtca ggatcattga tccattccag ttcaggccac agtttcttta
      661 tctcctcggt catgtcctgt tactttagtc gtgcaatgat ggcatcggtc atctggattg
      721 ttgaggctgc tcccatcgta accacttcgg gcttgcccgt catcttcatc atgtcatagg
      781 tcttgacctt tccttcttcg atgacttcgg cgatagccct gcgtatcctt gtggctgtct
      841 ccttctcgtc tatgtagtca agcatcatgc aagccgactc aatcattgcc acagggttaa
      901 cgataggcac ctcatacccg gcgtatttgg gagctgatcc gtgggtaggt tcaaacaccc
      961 ctatcccgtc ttctgagaac tgtgcgctgc aagcaaatcc gagtccgccg atgagaccgg
     1021 caaacgcatc ggaaacgata tcgccgaaca tattaccggc aactattacg ccataatcct
     1081 caggattctt tgtgagccac atcatctgtg cgtcgatatt tgtgttccag agcaggatgc
     1141 cggggaactc attcttctgc atctcctttg ctatcttcca catgaggccg gatgtttcac
     1201 gtatcacgtt tggtttttcg cagacagtga ctgatttgta tccgaatttc acggcatgtt
     1261 caaaagctgc cctgacgatc cgttcggtat atttctttgt gaagatcctt gttgagactg
     1321 ccagttcctc tttcgggcac catccgaagt tttcacggaa cttgggatgg gtcatcagcg
     1381 catcatagac ctgctgcgga ggattggacc attccacacc accgtagagc ccttcggtat
     1441 tctgcctgaa tatcaccaca tccaccatcg gttcctcggg atttccggtc ttgtcgcggc
     1501 ggatgaagtt gagggggttg cccttgtagc tgcggcacgg cctgacgcag atatcgaggc
     1561 cgaagtgttg cctgaggcca acaatcgggc tgctgtaggt aaagcctttt ccctgtagag
     1621 ccggtgaaag ctcggcagcg gcatcgtctt ttggtttgga ggtgatggca ccgaagagtg
     1681 cgatcttatg ttcagcaatc aggtcaatag tacgccgggg aagaggatta ccctcgcggc
     1741 accagaaatc ccacccgatg tctccgtata cataatcagc atcaaaacct gccgcatcga
     1801 ggacgcggac tgcctgcttc aggacaacgg caccaatgcc gtcacccggc agtgccacga
     1861 tagtacgttt ggccataatt ttattcttta aactgtatta tttcgtttcc cgtaaaaata
     1921 tagtttttcc tgagttaaaa aaaagagttt cagcaggtaa aattcataaa ggttgcaacg
     1981 gaaaaaaact atatttgccg ccaagtcatt caacttaaag aagaagaaca gaatgggtga
     2041 actgggggag atgaagccta ggaagctttg gggatatttt gaggagattt gcaaaatacc
     2101 gaggctgtcg aagaacgaag gaaggatcag ggcatacctg cttgaattcg cgaagcagca
     2161 taaccttgag gcaaaggaag accatgtcgg gaacatattg atagtaaggg aggctgctcc
     2221 gggcaaggag aaggtcatga cagtggttct tcagagccac ctggatatgg taggtgaaaa
     2281 gaatgcctcc catccgcata actgggcaga ggatcctata ttaccggtaa ttgacggcag
     2341 gtgggtcacc gccagcggga cgacacttgg tgctgatgat ggtataggta ttgcagccca
     2401 gatggctgtg cttacagatc cctacattca gtgcggacgg atcgagtgcc tgtttaccgt
     2461 tgatgaggag tcagggatga ccggagcaat caacctcaag cccgattttt tcacaggacg
     2521 catattattg aacctcgact cggaagatga gggcattctc ttcatcggct gtgccggagg
     2581 gattgacacc caggcacttc tgccctacac tccggagagg attacttcag ggcacagggc
     2641 catgaacatc tctgtcaccg gtctgcatgg cggccattca ggcgatgaga tacacaaggg
     2701 ctacggcaat tccataaaga taatatccag gctgttgcag gatcttgaga agaagtatga
     2761 catcaggatc agcaatttct ccggcggcaa cctccgcaat gcaataccga gggaagcgtt
     2821 tgtcacggtg acattcaatc cgacccatga agaaaatata ctggcagaca cggcagctta
     2881 ccataccctt gtcagggatg agtacaacgg cctggagcct gacctgaagg tggcagcaac
     2941 accggcagat attcctgagt tggtcattga cggcggcaca cagcacaggc tgctgggtgc
     3001 attgagcata gtgcctcacg gtgtgctcgg ctggtcaaag gagatacctg acctggttga
     3061 gacatcaagc aacctggcaa cgataaggct caccgatgac aacatgatcc acgttgtcac
     3121 cactcagagg agctcttcag aagaggcaaa gcactatgcc gcgatgaagg ctgagacaag
     3181 ctttgacctc gcaggggcga aggtgaccca ttctgacggt taccccggct ggcagccgaa
     3241 catgaactcg gcaatcctgc ggctgatgat tgattcatac cgcaagcttt tcggaaaggt
     3301 gccgcaggtt aaggccattc atgcaggact tgagtgcgga ctgatcctcg agaaatacaa
     3361 ggggatagac atgatctcat tcgggcctac catcaggggt gctcataccc cagaggagag
     3421 gatagagatt gagactgtcc agatgttctg ggatctgctt atcgatgtgc tgaggcacat
     3481 ccccgaagag taatatcagc tgacctcctc gaattcatca tggggtgcgc cacaaacggg
     3541 gcatgcccat cctgacggaa gatcttcaaa gggtgttccc ggttccacgt cactgtgagg
     3601 gtcacccact tccgggtcat atatgtagcc gcatacgggg catctgtatt tgttcataat
     3661 aattgttttg atattatctc ttcacttttc tgacagcatc aataaccgta ccgtcgaccc
     3721 atttgatggc agccaccacc ctgtcgtcaa aagcagcttt ttccggcttg ccgcacatct
     3781 tctcagcgat atccttcagt tcttcgattg tcaccagggg gagaccctta ccgcggacag
     3841 aatcaatcag gtcctgcctg cgggggttaa tggcaatgcc cctctcggta atgatcacat
     3901 caataagttc gcccggtccg cagagggtgg tcacccggtc tacgatgacc gggatcctgt
     3961 cacggaaaag gggtacggga attatgacgt tgcgtgcatg aaggcagttc tgccatcctc
     4021 cgattccatg caacagaagg ccgtcagagt gggtcaccac attcccgtta aagccggtgt
     4081 caatctcggt tgccccaagg atcatgatgt ccatcatggt ggtaaggttc cccttcgagt
     4141 ggtagttgta ggcgttgaac accgggtagg gaatgtgatt caggtttttt tcgattgact
     4201 tgactgaatc aaggtcaaaa gcctgggcat caaggatata tcccatctgg ccctcctcga
     4261 gcatgtcaac catgtacttg gtgctgccac cgaagccgaa actgagtttc cagccctttt
     4321 tcttcaggag ctggtgcaca tataccgcaa ttgcaagact ggtccctcct gccccggcct
     4381 gcatggttgc cccgttgcgg agcagacccg acttctcgag aaaagatgtt gtcagttctg
     4441 caatcagaag cctgtcaggg ctcttggtga tctgggtggt tcccgagact attttttctg
     4501 gaatacctat tttgtcaacc accaccacat aatcaacata attgccctct atctccatcg
     4561 ggaagcaggg caggtcaacg aggttatcgg tcaccactat caccttgtcg gcatacaggt
     4621 aatcaggctt ggcgtagcct atgacgcctg ttgcggatgg tccgcccgtt cccctggcat
     4681 tgccgaagga gtcagcggcg ggggctgcca gtatggcgat atctatcttc acgtcgccgt
     4741 cctgtattgc ctgtacacgt cctccgtgag agcgcagcac agccatcccc ttcatttttc
     4801 cttctgacac gaacatgccc agcggaccgt tcatgcttcc ttcaatgcgg ttgacagtgc
     4861 cgtcttccag gtattggatg acagggtcat tgcaggggaa tgatgctgac ggcagccaga
     4921 caaggtcttt cacgcccatt tcagaggcga tggtgaatat ctggttgctt accaggtcac
     4981 cgtcacgcag atggtggtga gttgatacgg tcattccgtc tttcagtccg cagcgtacga
     5041 gggcttcctt aagggagcct actaccttgt tgttgtcctc ggggtaatca atacacgtcg
     5101 cgatcggagg tgcatatttc ctgcctgagg ggcggtattt gcccacgccc ctgtaaggga
     5161 tggcttcctt gccgtttatt tcgtccggta cctggcgtcc ggcagcgttt tctacaagtt
     5221 tcatgaccgg taatgaatta attttttgac ttttccagat ccatccagtt ctccggcagc
     5281 aggcccagcc tgacggcgag ggtgatagtc ttttcggccc tggccaccac cggcggatct
     5341 atcatttttg tgccgagagc gacaactccg agtccctttt cgagcgcatc gcggtatgcg
     5401 atgacaatct tctttgcctt ttccatctcc tccggtgagg gggagaatcc ctccctgata
     5461 acggctatct gcctcgggtg gatgcacccc attccctcga agcccatggc ctttgctgag
     5521 agtacgttct gtagcaatcc ttccatgtca ccaacgtcag agaagaccga atcgatcggc
     5581 tgaatgccgg ctgccttgga tgcaaccaca agacggttgc gggcatagag tgactctttg
     5641 ccttcttttg tccgttgcac tccgagatca gctgtgtagt cttccagacc tattgcaagg
     5701 gctactactg attcggcagc agtggcaatt tccatagcgc gttccactcc ggcggcactc
     5761 tcgatgattg gcatcaggaa caccatttca ttctgtcctt ccctttcctt tatttccctg
     5821 attctgttat cgactgcacg tacggtttca gcattttcgc actttgggag aagtaccagg
     5881 tggacgttct ggggtattaa catttcaagg tcatcgagtc cacgttcacc ctggtttatg
     5941 cgcaccattc tctcagcccc gtagaaattg acaaccctga gggcatttct gacgaggatc
     6001 cttgcctcat cctttctttc cggggccact gagtcttcca ggtcaagaat gacgccgtca
     6061 ggtgaatgaa gcccggcgtt gaggaacatt cccgggctgt ttccgggaag gtagagcctc
     6121 gagaacctga atctgtctct tgatgaggca taaaggttct cagcggccat ctccggtatg
     6181 aagctctttg cagtaccggt aagggctttt accgcagctt caatcctggc ggcaattaca
     6241 aacggcagcg cccctgaatc attcaccgag agcctggcat tttcaatgcc gaagtattga
     6301 agaaggctgc gacactgctc ttcgatggct ttgccgtaca tagtctttac cttcgatctg
     6361 agattcagct cgataccgcc tgtttcggtg agttcaagcg ttacttcaat gtcagaccgt
     6421 actttgggcc cggcattacc tgttattgcc tgtttcattt tctgctccct gaaatgaagt
     6481 tcaaagtaaa cttaagaaat tatccgagat agctgagcag tataccggct gcaattgcag
     6541 aaccgataac acctgccacg tttggtccca tcgcgtgcat gaggaggtga ttcgtcttgt
     6601 cagccttcag tccttccatt tccgaaacgc gtgcgctgtc aggcactgca gatactcccg
     6661 aattgccaat cagcggattt atcttgtcgc cttccgggag gaagaggttc atgatcttag
     6721 caaatccgac accggagaat gttgccacag caaatgctgc agcacccagg ccgaaaataa
     6781 gtactgactc cttagtcagg aagacgtcag cctgtgtcga cgcgccaacg gtaagaccga
     6841 tcataatagt gacaatgtct atcagtgatg tgcgtgcagt ctcagccagc ctttttgtga
     6901 cgcctgattc cttgagaagg tttccgaaaa agagcatccc cagcaggggc agtgcgcttg
     6961 gcgagatgaa gcatgtcagc agcaatccga atatcgggaa gaggattttt tcggtcttgg
     7021 tgactgcgcg cgggggcttc atacggatga gtctctcctt tttggtggta aggagtctca
     7081 tgatgggcgg ctgtattacc ggcaccaggg ccatgtatga gtaagccgcg atggctatcg
     7141 ggccaataag gttcttcacg gtggtgccgt cagacagtac gttcacgcca ttggccagcc
     7201 ttgaggacag aaagatagcc gtcgggccgt cagcgccgcc gatgatgcca attgcaccgg
     7261 cctctggttt ggcgaaaccg agcagcagag ctccgataaa ggttatgaat attccaacct
     7321 gtgccgctgc cccgagcaac agtagcctcg ggtttgagat cagcgaagag aagtcggtca
     7381 tcgcaccgat acccaggaag atcaatggag ggtatacccc cttaaggacc ccgaaataga
     7441 gataattcag aacgcttccc tgctgataaa taccaacctg caggttggta ccaccatcag
     7501 tgaagaaggg tatgtttcct atgagaatcc ccgtaccaat ggggattagc agcagaggct
     7561 catactcata tttgattgcc aggtatatga agataaggcc tattaccatc ataatcagat
     7621 ttccccttgt ggcattccgg aaacccgtga atgcccagaa ctgtttgagt ccctgcacaa
     7681 actgatgctc agtatcttcc cgggtttcct gttcctggtc ctgtaaggtt gttaccatgc
     7741 tgatggcgcc tgagtcagcc atggcctgct gcctgtcatg ctcaggtttg acgaatgcca
     7801 gccatgccag tgatgtaatc accagcaggg ccatcattgt ccacatcttt ctccttgcca
     7861 tggctaacct attaccatta gcacttcatc ctggaggact gactggccgg gagaaacgtt
     7921 aagggctgtc acctttccac ctgtctcagc cttaatggcg ttctccatct tcattgtctc
     7981 gagggtgcat actgtctggc ccggtttgac atcgtcaccc ggcctgacga ggacctggat
     8041 aatgactccc ggcagcgggg cttttatctc cgttgccttt ccggaagcag attcgggtgc
     8101 cggctttgtc tcttttaccg gccttgcatg tgccggagct gatgttacga tggttttagc
     8161 tttgggtatc tcgcgctgca tctctacatc atagacggtt ccgttcacct cgatctttgc
     8221 catgcttccc tcgatctcga gaacctcaac atcatactgg tttccgttta tcctgaattt
     8281 gaagtttttc attacactta cgggttattc ttttcttaaa ggtttttcat attgtacaat
     8341 ttggagctcc agggcgaata atgcctggag atgcgcttga tggtcaccac cccgctctcc
     8401 acgtcatgca gctcattgaa atactggtaa atggccattg cgatggctgc attgacctcg
     8461 cctgactgta aagtctttac ggtctgaacc tccgcaggat ccataccctt tttacgggca
     8521 aagttcctga gtctgaagtt caggataaat gtgaggatgt acctgaagaa ccagtatacg
     8581 atcaacaggg agaggaatac tattccgatg cccaccacaa ctacggtcca ggtgagctga
     8641 tcgagggcct gtgcaccctc ggtgagatat atccagttca tgatcatctt ttatcagagt
     8701 ggaatgttgc cgtgtttttt cattggtccc gggtctttct ttgtagagag tgtcctgagg
     8761 gccctgataa ccctgaatct tgtgttgcgg ggttcgatca catcatcgag gtacccgtac
     8821 ctggctgcct cgtaggggtt ggcaaacttg tcgcggtact cctgttcctt tttggccacg
     8881 tactccttgc gttcggcagc gtcagcgatc tcgctgatgg ttttaccttc aaggacctcg
     8941 atggctccct ggggacccat cactgctatc tcagcagttg gccatgcata gttaatatcg
     9001 cctttcagct gcttggatga catcacgcaa tgagctccgc cgtatgattt tcttagggtg
     9061 acggtcacct taggcacatt ggcttcgccg taggcgtaca tcagcttggc tccgtgaata
     9121 ataataccgc catattcctg ggttgagccg ggaaggaatc ccgggacatc gacgaaggtt
     9181 acgatgggga tgttgaatgc atcgcagaat cttatgaaac gtgcagcctt gcgcgatgcc
     9241 tcgatatcaa ggacacctgc gaggtatgcg ggctggtttg caactatgcc gacgggcatg
     9301 ccgtcaaacc tggcataacc gaccactatg ttcttggcat aggcttcatg gacctcgacg
     9361 aagtcatgat agtcgacaac ccttacaatc acatctttaa catcataagg ctggttgggg
     9421 ctttccggta tgatatcatt gagttcatcc tccagcctgt caatcgggtc atcacagtcc
     9481 agctgcgggg gttcctcgag gttattctga ggcatgaagc tcagcagttt cctgattaga
     9541 tcgagtcctt ccttctcctc atcaacggcg aagtgggcca ctccggactt tgaggtatga
     9601 acgccggctc cgccgaggtc gtcaattgta atatcctctc cggtaactgc ttttgccact
     9661 ttgggaccgg tgacgaacat gtagctgctc ttgcggctca tgatgatata gtcagtcagt
     9721 gccggggaat agactgctcc gccggcgcag gggccgaaga tggctgatat ctgagggata
     9781 acccctgaag caaggatatt gcgctcaaat atttccgcgt agcctgcgag gcttctgaca
     9841 ccctcctgga tacgggcacc gccggagtca ttgataccga tgaccggagc gcctgctttc
     9901 atggccttgt ccataatctt gcagatcttc tgggcgaatg tttctgacag agacccgccg
     9961 aagaccgtga aatcctgggc aaagacatag actaatcttc cgtcaatggt gccgtgacct
    10021 gtaatcacac cgtcagagag aaatttcttg tctgccagcc cgaagtcttc ggttctgtgg
    10081 gtaacgaaca tgtcaaactc ctcgaagctg ccttcatcga gcagcagggt aatgcgttcc
    10141 cttgctgtca gcttgccttt ggagtgctga tcctcaattc gtttttcacc gccgccctta
    10201 cgcgctttct ccctgtaatc aaggagttgt ttgattctat cctgactctt cattgaacct
    10261 ggttatttgt tgtttttgct gtttttgtcc tcgcacagtt cggtaagcac accgaaggtt
    10321 gatttgggat gcaggaaagc gatgctcagc ccctcggcgc cttttctggg agtgctgtca
    10381 ataagcctga tacctgcagc ctcggcctcc ctaagtttat cctcaatctg gtctacggca
    10441 aaggcaatgt gatgaattcc ttctcccctt ttctcgatga atttgcccac cggcccttcc
    10501 gggtcagttg actcgagcag ttcaatcttt gtctgcccga tcagaaaaaa agctgtcttg
    10561 actttctggt cagccacttc ctcgatgttg tagcacttca ggcccagaac cttctcatag
    10621 aacggaagtg catcggcaat ggaattgact gcaattccaa tgtgctcaat atgtgtaggt
    10681 ttcataaata atcgttaaaa atagagataa taataagagc atgcaagtta aacctattta
    10741 actcatcctc acacgataaa aatcatgttt tcaggtctca gaaggggaat tcggaaggca
    10801 cttccttcag ctcttcggtc tcaggtttgg aagcagcttc gtggatcggg ttaccgtcaa
    10861 cgaagatggc cctgaagggt cttgtggggc atgcgtactc gcaggctccg caaccaatgc
    10921 atatttcgtc ctttacctca ggtatgagca ggcgtccttc aaagggtacc atatggcatg
    10981 ccttggtggg gcaatgttca gagcaggctc cgcatgcagt cttttcagta ttgacaatgc
    11041 agttttcctt aataaagaca gccttgccga tctgagtcag tttcttggct tcgggcatga
    11101 gtggcatgat ggctcctgtg gggcagacgt cggtacaccg ggtgcaatca tagttgcaga
    11161 agccgctgtg aaaatccatc ctcggctgca taatgccggc aaaaccgtac tggttggttg
    11221 aaggctggag cacatttgtg gggcaggcat tgacgcacag ggtacatgcc gtgcacctgg
    11281 aggtgaacat ttcaatgccg gatgatccgg gaggtgatac cgggtagagc cggtcttccc
    11341 tgactgtaga atcctttgta ggtgagggtg tggtgccctg tgccttcagc aactgcgaac
    11401 cacctgccag gaggacgagt gatccggcaa tgaaaccgcg gcgggaagcg tcagtctcgt
    11461 caggattcat ctgttgccgc ttcagcctta ccaggccgta tgacatgccg ttttccttgc
    11521 atgcgtcaat acagttgaaa cagttgacac agcggctcag gtcaactgat ttgttcagga
    11581 aatctatgca tgatgacttg cacgccattg cacacctgcc gcatcgcgtg cacttatcct
    11641 catcgaatct gattctgaaa agtgacacct tgctcaggag gccaagcagt gttcccacag
    11701 ggcagacagt gttgcaatag agtctgccgt acctgaaggc aagcagcccg gtcagcagca
    11761 ggaatgctgc tggaattatg taagcagcga gctcgtaggg tttgatatcc acgcggtaaa
    11821 ccgaatagat atcgaatttg cccagaattc ctgcgagcag attgttgatg acaagcacca
    11881 ccggctgtac aaagtaggtc atcagcctcc cgaaggtact gtacggatca aggattgtga
    11941 gcacatagac accgccgata gccaggagcg ctgctgttat cacaaggatg gagtagcgga
    12001 ggatggtgta gggttttttg taaccgtact tgcggaaccg cctctttacc agtcctcctg
    12061 cccggctgaa cgtgtcctga agtatgccca gcgggcagag aaatgaacag taagtcctcc
    12121 cggtaaggag ggtcagaata aggacgatta tgaacccggt tgaggtgaca acgccggcaa
    12181 tgacaaactt gaggaatgac ggcacaaact gaagataaag gatcgggccg aaggccttat
    12241 caccaaggac tcccctgaag tcgacaaaca gaaatacaaa cagaagaagt acgaggacag
    12301 caaatactgt acggatgtgt ttcagggcag aggatttcat gaatggttac attttcatcc
    12361 tgtgtatgtt cattgttgca aggtccattt ttcctatgcc cattcctgct gcaagtgcta
    12421 tatgttctac agatgaaggt tcctttccca ttagttttgc acccgctgcg tctatggcca
    12481 cccagtcagt ggagagcatg agtgacttca tgtttgagag gtcagccaca gacaccccac
    12541 ggggtccgtt acgcatcatc acgttatagg catccatgac atggaggttc ggcttcttcg
    12601 aatagagagc atagtcagca atgcactggt tgagatggtt tctgtgccag taaccacggt
    12661 cccataccac ccccatcatg ttcttgaggg cgccggtcat catagccgag ctgtggtgct
    12721 taaggatggg gacgttgatg aagacatcgc tcgaaagaac aagttcgtga actttagctt
    12781 tcgtcagctt cttcccgttg ggaatggtca cctcctggta atagctttcc gagttgcccg
    12841 gcaccatctt tgcgccggca tccttgacgg cctgttcaat ggcggagttc ttgtagcatt
    12901 tgatccagtt atcgcatgta ttgtcaaata caaaaacctc cttcgctcct gcacggtaac
    12961 aatgctcaat aattctcttt acgagagcag ggttggtgtt ccctcctttt tcaggcacca
    13021 cgtcccagcc gatgtttggt ttcacaagca ccttctgtcc tttcttaaca aaagtaccca
    13081 tgccgcccag tgcctgtatg cccatgtcaa acattgcagc cggttctccg cccatgactg
    13141 caataaggtc atagtcagca cctgagggaa gacgcttccc tgcaaatgcc ttctcataac
    13201 cgccaaaact cataaaggct cctgccgcca ggccggcacc aagtgaactt cgtaagaaat
    13261 ttctcctttt cattcccttc tgtttgatat taccccaaaa atgagaggat gcaaagtaaa
    13321 catttttttt aatatatcgg ctccgtattc ttatattagc agcctgagaa tacggtctta
    13381 agcgattatg cgctgccggc aggtgatgtt aaaaaaacta aaaaaaatga agatactgta
    13441 ttatgactgc ttttcaggca tcagcggaga catgaacctg ggtgcgctgc ttgacctggg
    13501 tgtaagcagc gaaatcctga ccgacggtct gcagaaactt aatacgggtg gctggaagct
    13561 ggatatcagt cgggaccaga gacatggcat caccggcact aaagttaccg tcatcacaga
    13621 tcataatgat catgagcaca cgtacggaaa tgctcatgat catgaccatc accatgacca
    13681 tgagttcaat catggtcaca atcataagca cgatcatcag cacaatcatg agcatcgcta
    13741 tgaccatcag catgagcaca ggaaccttgc cgacatagaa aagataatta aggggagcgg
    13801 tctgccggct ccggtcagtg atctggcgat gaagatattc aggcatatag ccgaagctga
    13861 agcagctgtc catgacaagc ccttgcatga gattcatttt catgaggtgg gtgcaattga
    13921 ctccattgtt gacatagtgg gtgcagccat ttgcctcaat gagctcggag cagacagggt
    13981 ttatgtttcg gagattgagc tcgggagcgg catggtaagg tgccagcacg gactgctgcc
    14041 tgtccctgca cctgcaacag cacggatcat cagcggattc ccggttcata tcggcggagt
    14101 ggattttgaa gccaccaccc caaccggggc tgcaattatt gccgccattg cagaaccatt
    14161 gccgccctca atgaggatgg tcatcaggaa gagcggttac ggcattggtc agaagaacaa
    14221 tccttcaagg cccaacatac tcagggttag tctggcggag acagccggtg aagctgagaa
    14281 agggcattcg gcaagacttg ttgaatgcaa tatggatgac atgaatcctg agatgtcgga
    14341 atatatttcc ggcaggcttt tcgccgcagg cgcaggagac gtatggttca caccggtgat
    14401 aatgaaaaag gggcggcctg ccttcaccct gagcgtcata tgtgaggagg atcagctcaa
    14461 tgctgtcagg gagatactgt tcaccgagtc aaccacaatc gggctgaggg tcctgtcctt
    14521 ctccaaggag acgcttcacc gcgaatttga agaggttgaa acaaggtttg gaaaggtaac
    14581 ggtcaagaag tcatacttca acggcaggct ggtctcggtc aagccggaag cggacaggtg
    14641 tgcagccatt gcagccacga tgggaatacc gatgaaaaac gtgatccggg aaataacctc
    14701 gctaaccagg gaagaatcat gaccgttgca ctgaaacttg acagactgaa gtcgatcctt
    14761 tccgaatcag gttcggcagt aatagccctg tcaggaggca ctgacagcac attccttgcc
    14821 agtgttgctg cagaggtgcc cggactccgc atgatggcag tgacggtaag caccccgtat
    14881 atgttctcat cggaggttga tgaggcttcc cgcttctgct cggagaaggg cattgcccac
    14941 cggcaggccg ggtttgacat tccgccttct gttgcgttaa atcctcccga ccggtgttat
    15001 ctgtgcaaga aggaggttat gaaagcgatc tcctctgtgg cgaaggaaga gggatatgca
    15061 tttatctttg acggcaccaa cgcagatgac ctgcttgatt accgtccggg actgagggct
    15121 ctcgcggaag agggtataag aagtccgctt gccgaagccc gtctgacaaa ggatgagata
    15181 agggcgctgg ccagggtggc cggcctcgag gtagctgaca gaccctcgaa cacctgcctg
    15241 ctgacccgct ttccccatga cacggagatt aaacccgccg atctcaggaa ggccggggag
    15301 gctgaaagga tactgaagga agcaggattc tcaggcgccc ggctcagggt gcacggtgaa
    15361 ctggtcaggc ttgagctgat gagagagcag tttgaactga ttgccctgtc tgaaacaaga
    15421 atggcaattg ctgctgcact gaaaaagctt ggttacaggt atataacaat tgacgctgaa
    15481 gggtaccgga gcggaagcat gaacaaaacg acgaaagaat gaattcatca gagcttgaaa
    15541 agatactgaa ggaaatcagg accgggaagg tgagtgttga agaggcgatg gagaagcttc
    15601 gtgactttcc ctatacagac ctgggatttg cacgcattga ccatcaccgg gagctgagga
    15661 cagggtaccc tgaaatagta tactgtgccg gcaaaacccc ggagcaggtc agggccattt
    15721 tcaaggccat ggagggacac gacaataata taataggtac cagggccgta aaggagatgt
    15781 tcaactatat cagtcccttt tttcccgaag cagtctatta tgagactgca cggataatca
    15841 gcataaggag aaaggagata gttccccctg tatcgaaaat agcaatcatt actgccggta
    15901 cctccgacat ccctgttgcc gaggaagctg ccgtcactgc cgagctgctc ggaaacgagg
    15961 tggtaaggat ctatgatgcc ggtgtggccg ggatacacag gctggttgac aagcttcctg
    16021 agataaggaa ctgcagggta tctgtggtga ttgcgggcat ggagggagca cttgcgagcg
    16081 tggttggcgg actggtaaac atgccggtga tagccgtacc caccagtgtg ggttacggtg
    16141 cctcctttga aggtatctct gcgctgctgg caatgatgac aagctgtgca gccggcgtta
    16201 ctgtggtgaa tatagacaac ggtttcgggg caggctttgc ggccagccgg atcaacaggc
    16261 tctgattgcc atggctgatg agctcctcct ttcaaccgca tacttccctc ctgccgggta
    16321 tatgagtctg attgcctgca gcatgggtgt gctgattgaa cgggaggaaa actatcacaa
    16381 gcagacctgg cgtaacaggt gccttgtcat gagtgcaaac ggtccgctgc ccctggttgt
    16441 tccggtactc aggggatcat tccataagac agcgataagg gatctgagga tagacaacag
    16501 cagacgatgg aaggaactgc atctcagggg aattgtttcc ggctatgcgg ccgctccatt
    16561 ctttgagttt tactttgatc tcatatcagc ggtgttgtca aagacacacc catttctgct
    16621 tgatcttaac agtgaggcac ttgaggcggt atgccgggcc acgggcattg atgtgccggt
    16681 cggttacact gaccgctatg agcctgaggg cacacgggaa aatgactaca ggtaccggat
    16741 cactcccaaa aagccgtcag agatacccgg gtacagggat ctgccctata cgcaggtctt
    16801 cggagagaaa caaggtttcg ttccggggct gagcataata gacatgctgc ttaataacgg
    16861 cccagggacc cgagcgttgt tgctgaggtc cctgggagca gacaactgtt aacaacttta
    16921 gaacttcagt cccagagtga ctgacaggta cttatccttt atattcagat taaccggatc
    16981 actggtgtat tccgggactg cccttgaatc atcggggtac atcatgtatg attcatagct
    17041 gttgagccac accattgcca cgtcgatgta gaaattatcc tgcctgtatc ccaaccctgc
    17101 gctgtatccg tttgatgaag catcctcatt cagtgttcct tcagtgaatg ctgaaccgtg
    17161 atggctgtat ccgcccctca ggtagagggg tccgaggcgt gcctcggcgc caagcctgag
    17221 gttgcctgta gcacccacct cggcagccag gtcttcgttt tcggtctcga agttatagcc
    17281 gtcagccccc ttgctaagcc gggcggtggc ataatcgatg aactcataat cagcgctgat
    17341 cagtccgaag ttacctatct ggtacgcgat acctgcattc aggtgataag gggttgtcac
    17401 acggtaacgg tatgtcatat cttcattgtt gacaaccggg ttggcatcgt cagaaggatc
    17461 ggccggtgtg tcattgtcaa gcttgactga cagactgtta taaaaaacct catcgatagt
    17521 gtagatggta ggtgtggtga aacctacacc gatgcggagt gattcgaaag gtcttacaat
    17581 tgctcccagc ttgaagttcc agcctgaacc cgtagccttg aaatgatcgg tataggtgaa
    17641 attcacaaag tcgaaggtat tctgtgcatc atctgactcc agatggctgt aatgaccggt
    17701 atatgagaca ttggtgatac cgaatccggc tcccagataa accttgtcgc caaggctggc
    17761 gccaagggca atggtatgct cattgctgta tcccgagtta tcaatgactc tcttcagttt
    17821 ctgtccatat tcgggataag tttccccgta ataagagaag attgatgcgt attcatcgag
    17881 ataattcgga agggtatcaa taaggtatgt atcatatgcc ataaatgccg taccgccgag
    17941 ttcgccggtt ctgtagccgg tggcctgcag ggcccagaag tcagccatgg aactgttgtt
    18001 gctgatgccg tcaatgactg cattcctgta gtaattattg gttctgttga aggtataggc
    18061 aaggttcagg tttgtgagtc cttttccgct gcctgtacgg aatgatgaaa ccaatccggc
    18121 ctggccgaga gtcagtccgg agtagctgtc gtcagagccg tatccgttcc agtcagtgtt
    18181 caggtcgcgg aagaagacag cgggagtgat tgatacttct gttgagcgga agacgcctgc
    18241 cgctgcgggg ttgagtccaa tcgccgacac gtcgcctccg agtgcggtaa aagcgccgct
    18301 cataccgttg aaccttgcgg ttccctggta gaatagcctt gagtacctca gtgcatcatc
    18361 catgttctgt ccggccagcc aggcaggaag catcagaatt gatgcggcaa tcagaaatat
    18421 ttttcttttc attttcttga attttaaact atacacttcc tgttgctcct gcagactgcg
    18481 gaacatttat cagcatttat tgacctgtat ttttaccttc tgccgcctga gctgctcctg
    18541 ccacctgatg aagaggagga agaggatgat gaggatgatg aggatgatga gcttccggag
    18601 ctatagcttc cgcctgatga actggagctt cccgaactgt agcttctgct tcccgaagag
    18661 tacgatgacc cgctgcttct gcttcccgaa gagtatgagg aaccgctgct gctgcgtgaa
    18721 ggagcagagt actgcaccgg tgatgatgac gaccttgagg gtgtgtatgc cggggtagtg
    18781 ctgctgcttc gcggggtact ggttctgttg tataccgttc cgctgctctg ggttgtggta
    18841 ccgctgttgc tgcgggtagt gttgtatgac ggtcttgagc ttaccctcgg gttgttgtag
    18901 ctcggggtat agcttctctc agcaggctgg tactggggcc tgtttacagt ctgtgtctgt
    18961 gtctgtgtcc tgaccggtgt ctgctgctgt gtccgggtct gagtggtggt ttcagtcctc
    19021 acaggagact gtgtacgtgt ctgagtctgg ttctggacgg tggtgttatc cgattcgcgt
    19081 cttgtatatt caggtcttga gacggttgca gcgccctgat tggaattgct gacagttcca
    19141 tcctgggttc tgcgggtacc ggttgttgtt ccggcagcag cggcttccct gttgggggag
    19201 gattcagtgg ctgtcctcct tgaaccggcg ggagtgttgc ctgtcccggc cggcaatgcg
    19261 gtgcctgaca catcccttct gctgttcatg tcacctgaag gtgcatagcc tgacttggtc
    19321 ctgatatcag tgttggagta tgatcctctt gacatggagc tgtatccgcc tctgcgggcg
    19381 atattttcca catatccgcc atcattgtaa tgtgaggggt atccgtagcc ggagtaccag
    19441 ggatagtaac cggggtatcc gtaaccgtaa tagccaccgt aataattata ataggaatac
    19501 gggctgtaac cccatgagta gtacgggctg tagtatgaat agggagagta ataatcatag
    19561 tagaagggat cataacccca ggaatagggt gatccgtaag atccccagta cccatagccg
    19621 aagccgagtc tcatcgagaa cggtgagtag tagaacggat ctctccagta aggatagaag
    19681 taatcatcat tgaagaggta tatcctgtcg gccgcaccac cgtaatagtt atttactatt
    19741 gtcggttcgc cggctgcagc gtatgccgaa tactcatcat ccggaacgaa ttcgtcagcg
    19801 atgagtgtat ctcctgcata gatattgtca taataggact gtccggcatt cctgtcctgg
    19861 accgtgggac cagccacagc caccggattc tcagacggcc tgtaatacag gtcatcgttg
    19921 ttggctcctg tgaagcggag tgatgaacac gcgcttgtta tcacaagtat tgccgggatt
    19981 atgtaccaaa accttttcat ttcaatctcc ttctggttat ctatccaaaa ttatacaaat
    20041 tccgtaccaa tttcactcag caggccagtt atttataatg caccgaaata agtaaaaact
    20101 cagcatcata atcaggctca ctttccggtt cacaagattt gtctattttt acgccatcat
    20161 ttttgagaat taacaataat acaaaattat aactgaggaa tggccaaatt tcttactaac
    20221 agggaggaga attattcaca gtggtacaat gacctggtga taaaggctga catggctgag
    20281 aactcggccg tgcgtggttg catggtcatc aagccttacg gatacgcgat atgggaaaaa
    20341 atacagtcgg aacttgaccg gatgttcaag gccacaggtc atgtcaatgc atatttcccg
    20401 ctcttcattc ccaaatcatt cttcagccgc gaggctgccc atgtggaggg atttgccaag
    20461 gagtgtgccg ttgtaaccca ttacaggctc aagaactctc cggacggcaa aggcattgtt
    20521 gttgaccctg atgcccgtct ggaggaggaa ctcatcgtga gaccaacatc ggaaacaatt
    20581 atctggaaca catacaagga ctggatccag tcataccgtg acctgccgct gcttatcaat
    20641 cagtgggcca atgtggtaag gtgggagatg cggacgaggc ttttcctccg taccaccgaa
    20701 ttcctgtggc aggagggtca tacggctcac gccacaaggc aggaagccga agaggaggct
    20761 gtaaggatac tgaatctcta tactgacttc gttgagaatt acatgggagt gcctgtaatc
    20821 aagggggtaa agactgagag cgagaagttt gccggtgcca ttgataccta tacaatcgaa
    20881 atgctcatgc aggacggcaa agcgctgcag ggcggaacaa gccacttcct cgggcaaaat
    20941 ttcgcaaagg catttgatgt gcagtttaca aaccaggagg gcaagctcga ttatgtctgg
    21001 gcaacctcat ggggagtcac cacccgcctg atcggcgcac tgataatggc tcacgccgac
    21061 aataacggac tggtgctccc gccgaagctg gctcctatcc aggttgtgat agtcccaatt
    21121 tacaaaggca acgatcagct tgacaaaatc tccgatatcg ccgttgatat caggcaacag
    21181 cttgaaaagg cagggctcac tgtcaagtat gacaaccgtg acacccagaa gcccggattc
    21241 aaatttgctg aatacgagct taaaggagtt ccggtgaggc tggcgatagg tccccgcgat
    21301 gcggaagagg gcaccgtgga ggtagcccgc cgtgacaccc tgaccaagga gaccatcagg
    21361 cgcgaaaacg tcgtggcaca tgtcatcggt ctcatggaag acatacagca gaacattttc
    21421 aggaaagctg ttgaattcag ggaaaacaac accttcacgg ctgatacctg ggaggagttc
    21481 acggacatca tcgaaaacag gggcggcttc gttcttgcac actgggacgg cacaccggag
    21541 actgaagaaa aaataaagga cctcaccaag gccaccatca gggcgatacc ccttgacagc
    21601 agggaagaaa agggcaaatg tatcctttcc ggcaatcctt caaaccagag agtattgttc
    21661 gcaagagcct actaaacaga ttattgcgtc agacaggcat aggtcaccca cggatttact
    21721 ccatgaggta acttatgttt taatggatac cggttgtttt tgtttgaaat aaatctgtaa
    21781 ttttagacaa ttcagcaatt gaacagttat ggcggatgag attacacaaa gcaagattct
    21841 gattgtagac actctgaagg gaaaatctgt actcaagcag aaggtatttg acaataccgc
    21901 agtagccttc atgatggtga aggatatact gaagaatctt gcccgggatg tgaacggcaa
    21961 cctgggtgga gttgaccaga ggatacgcat ggagtacact gacaggagca cctttgatgc
    22021 tcagctcaag gtagcggccg atcttctttt gttcagcatg cactcgaaca ttttccagtt
    22081 tgaccgcgaa catcccgcct ggaagacggc atacatacag aagaacaagt tcaatgcgta
    22141 cagcggcata ataaacatat acaacttcct tgcagattca ttcaagtatt cgaggcttga
    22201 tgaccttggg taccttattg cccggatctt tatcaatcat gagaaccagt attttgtaga
    22261 aggcaagagg cagatgggga tgctgttcac caattacggc aacgaaccct tcagtcagga
    22321 atcgctgaag aagattgttt atacggccat caattactca cttgaatttg accttctggt
    22381 tcctccgtat gacacggtga aaattgcttc agtaggccag gcagaatcaa agatacagca
    22441 ttcacggctg attaccggaa aaagactcgg gttccagttt aactctgatg atattctgga
    22501 tgacaattca aagccaaagt gaatccattc acaaatagga cattcaaaac tttatttttc
    22561 ccctttctga cgctgtttat tatcctgcac gggctccccg tagctgctca gggcaccagt
    22621 gctcccgaca ctctgtcaag ggctttctat gactccctta aggtaagggc tgaaaaacgc
    22681 aggctgacga gtctccttta tgacatgatc attgtcacgc cggctcctcc cgggcaggcg
    22741 agggaaaaac tggccagcac cacggcctat gaagcgtatg agggaaggaa gatccgcaac
    22801 atagagataa tcaggctcga cgcattcgga cagaatatag acaatcctga ggggaagact
    22861 ccttcacgag cggagaggct tatgaactcc acctatacca agaccaggag gtttgtgctg
    22921 atccggaacc tcgtattcaa gacgggcgac acaatttcat cactgaagct gtctgacaat
    22981 gagcgacttc tcagagagct gccttttatt gatgattccc ggatcacggt agtgccggca
    23041 gacagcaatt acgctgacat tgccgttgtt gtgcgtgaga aatacccggt gggcgccaat
    23101 ctgcgcatcg atgacatcag gagcggcagg gtgggcgtct tcacgaggaa ttttgtcgga
    23161 ctgggccatg aactcgagat cagtgctcca tatgactata acgagtacga cgcgcccgga
    23221 atagggctcc ggtattcaat caacaatatt gcccgcacct tttccagact gaacatgcag
    23281 ttttcagacg ggctgggaac cacgcatatg ggaggaacct tcagcaggga ttttgtaaca
    23341 tccgaaacca ggtatgcatg gtcagcttcc gtaatattca cccgtaccaa tgatgaaatt
    23401 gacaccatga ccgtgccggc tcccttgagg ttcaccaggc aggattaccg ggcggcaagg
    23461 tcattcatgc tcgacaggaa cactgtgact aggctgatag tcgcgggcag gtacatgcac
    23521 aacaatgtat tcaggagacc cagcattgat gagttttcat attatcgctt tcagaactac
    23581 cagtcagtca ccgggagcct tgcagtctcc tcccagcgat tcctaaacac atcattgata
    23641 tatagttacg gtcgtactga ggatatcccg tacggttata tggcagaact gttccttggc
    23701 cgtgaaaaga atgaattcaa gtggaggaac tatgcaggag gaacggttgc gtacggcaat
    23761 ttttttaccg gggtcggtta tttgtacgcc ggagccggca tatcagcttt ttacaataac
    23821 gggcacacgg agcagggtat gtttcaggga agcataaggt atttcacccc actggcccgg
    23881 gtggggcagt caagggtccg tactttcata aacctgaact acgccagggg tttcaacagg
    23941 tattccgatg aattcctgta tttcagggat gatggtctta taaggggttt ccgtaatgac
    24001 tccctgggcg gtaacacgcg gatcacagta tcggtggaac cggttctgtt catccataaa
    24061 ccggtgattg gattccggtt tgccgttttt gcctttgccg atgcgggttt attgtccagg
    24121 ggccggcaga tgggagcaga ctattatacc gtagccgctc tcggagccgg ggtcaggatc
    24181 aggaatgacc agcttgtgct taataccctg caggtaagga tcgcgtggta tcccaactct
    24241 cccccgtggt cagtgagctc cctggttact gccaacagta tcatgcgcct caggcctccg
    24301 ggttttgaac ctggtccccc ggggctggtc ccgtacctgt aagcctccgg tgattccgcg
    24361 gacctgccgc atctgagctt tttcgcggaa cgcgacactt ttaccatgat gatttttaac
    24421 tttgcagaaa gcaggtggtt atgctcagca gatttctgga attttccgaa agggaaaagc
    24481 tcttcaccag gggcagcagg ctcctgattg ccgtaagcgg cggcattgac tcaatggtga
    24541 tggcctggct tttcagagag gccggcattg agtactctat tgcccactgc aacttttcac
    24601 tcaggggaga tgagtctgac ggtgatgagg agttcgtcgc ggcatatgcc cgcacaagca
    24661 gcatttcatt cttcagcagg aggtttgata ctatgacata cgccggcact cacaggatct
    24721 cggttcagat ggctgcaaga gaactgcggt acgactggtt caggtctctc atccgctccg
    24781 aaggctttga ttccgtggct gtggcgcata acctgaatga caatgtggaa accttcctga
    24841 tcaacctgtc gaggggaaca ggccttcacg gactgaccgg tatgagcccg cgcacgggag
    24901 acatcatcag acccctgctc tttgcaacca gggaggaaat tgcttcatat gcctccgcca
    24961 ggggcataga tttcagggag gacaggtcaa acgccgaggt aaagtatatc cgtaacagga
    25021 tcagacacaa ggttatcccg gagttggaaa aggtcaaccc cgggattctg gcggcagtgg
    25081 ctgataccat gaagcacctt tcctccgact cgtcactggt ggatacgtat ctttcgcagc
    25141 tcagcggaca gctattcacc ttctctggtg actccgccgg ggttgaaatc agcagtctca
    25201 tggctttgaa tccattggaa ccgcacatct tcgggctttt ccgcccgttc ggactctttc
    25261 cgaaacagac tgatgaggtc atctctcttc tccagtcagg gacaggcagg tctgtttaca
    25321 cggctaccca ccggcttctg aacgacaggg gacggataat catttcaccg cggatatccg
    25381 aagctcctga agaatacaca ttcaattcca ttgacgagat tcgaatatcg ggtctgttca
    25441 gtgccttaag aatcacagag ccttctgacg atgcactgcc cgccactcac ctgaccgcat
    25501 cccttgatct tgacctggtg gctttcccgg tcacggtgag accatggaag ccgggcgaca
    25561 ggtttatgcc actggggatg aaacagatga agaaaatcag tgatttcctg atagatctga
    25621 aagtgccggt gacagagaag gagcaggttt tgctgctgct gtcaggcagt gatgtcatgt
    25681 gggtaatggg ttacaggata gatgaccggt tcagggtcag tgaagagacc gggaagatca
    25741 ttgttcttac cctttgaaca gtcgcacctg gcttccggca aggctcactc agcggtctct
    25801 cattccgttt attcccctgt caatcatcag caggtcagcc aggccaattg ccacggctga
    25861 ttccaggata acagggatgc gccgcgcaat gcatgcatca tgcctgccgg ttatctcaag
    25921 gtcggtcatt tctccggttg ccgtgtcaaa tgttttctgg gggacacctg tacttgcagc
    25981 aggtttgact gagaccctga tgataaggtc attgccgttg gtaattccgc cgttgatgcc
    26041 accggcattg ttggtggatg ttttgcctct tgcatcaata aacgggtcat tgtggagtga
    26101 accatagctt ctcgacgagg cgaatcctgc cccaaactcc accccgttca ctgccggaat
    26161 cccgaaggca atcattccca gggctgcctc cactgacatg aacgagggtt ccccgatgcc
    26221 tttcggaaca tttttcacca cgcactccac cactcctccg acggtgtcat tggtttccat
    26281 tgcccttgca atggccgacg tgatatcctt ctcccctcct gcttccacca gggtggctga
    26341 tattattgca ggcgacagaa tcttgcgggc aaagtaaccg gcagcgacga tgccccatgt
    26401 aaggcggccc gagaaatgtc ctcctccgcg catgtcgctg aaacctttat acttgaatcc
    26461 ggctgtaaag tcagcatgac caggccgcgg tatgctggtg aactgttcat agtcgccggg
    26521 acggaagtcg ttgttgcggg tgcagatcac cagaggggcg cctgttgtac agtcattgaa
    26581 aataccgctt atcacctccg gttcatccgt ctccctgcgg gtggttgttc ccggcagcaa
    26641 tcccttcctc cgggaaaggt ctgccatgag ttcctctcgc cagaagggca gacctgcagg
    26701 gcagccgtca atcaccactc ctactgccgg tccgtgagat tccccgaaca gtgataccct
    26761 gaatattgtt ccgatcgtgt tcatttttca agttataatt actgactttg actttccttt
    26821 tcgttataat agttgacaaa tgcctgcagt tcggtagttg aaatctttct gatgaccgtt
    26881 cttccgactg atttcagcaa aacgaagttc acccggcctg actcactttt cttgtccctg
    26941 gagatcatgc tgaccacgtc tggcggcagt ttatggcagg tcataaggcc ggccttttca
    27001 agggccgtct gcaggagtct gagttcagaa tgagccattt ctcccttcca tactgacaat
    27061 tccgcagcaa tggccattcc ctttgctaca gctactccgt gaggaatccc ggaatgcgtc
    27121 tcaatgacat ggccgaaggt atgcccgaaa ttgagtattc tcctctgccc tttctcaagc
    27181 ggatcatgtc ttaccactcc ggcttttacc ctgacagccc tgaggatcag gtcacctgtt
    27241 acagccgtgt ctgctgcaat gatgtgtccg gccgaagccg agatgtcaaa gaagaggttc
    27301 ctgtcacgaa tgactgcatg cttgaacagt tctcccattc ctgacctcac ctctgcctca
    27361 ggcagtgtgg tcagcattgc atggtcacac agaacgaact ccggctggcg gaaggtaccg
    27421 atggtattct tgtattctcc caggttcacg cccgtcttcc ctccgatact tgcatccacc
    27481 tgcgaaagca gggtggtgga aacgaatgcg tgcctcaccc ctctcatata aaccgaggcc
    27541 accatgcctg caatatcaca taccacccca ccgccaaatc cgaggatgaa tgatgaacgg
    27601 tcggcgcctg cctcaagcat cttactgcag atttcttcca ccatccgcag tgttttgctc
    27661 ttctcccccg gttcaattgc aataacctct ccgggtggga actttttccc gtacaacttt
    27721 tgcacatttc ggtcggttat aatgaagagt cccctctgcg ggagcagagt gacaacatca
    27781 tccactgttc ccccaagtat aataagggat tcccccccgg ggctgtttat tctgatccgg
    27841 ttcattccgg cacaaaataa aaaacagaac cggtttttaa catgatttta ttcagatagt
    27901 ggtacttttt cggcaaaaaa ataaaaaaac agtacattca acccctgaaa cagaagagaa
    27961 gagtgaggat agcagagaga tacagaggga tcatggaatg gtttgaggcg aacatgcctc
    28021 tgccggagac ggagctggag tacagtgatc cttttcagct gattgtggct gtgattctct
    28081 cggcccagtg cactgacagg agggtgaaca tgatcactcc cgctctattt gcgcgctacc
    28141 cggatgcgtc ttcaatggcc agggcaacac aggaagagat attcggactg atcaggagct
    28201 gttcctaccc gaataacaag gcgaagcatc tccgggggat ggctgagatg atcgttagcc
    28261 ggtttgatgg caaggttccc tcaacgcgtg aggaactgac catgttgccc ggggttggca
    28321 ggaagaccgc gaatgtcata gcctcggtgg tatggaacag tccggtcatt gccgttgaca
    28381 cgcacgtctt cagggttgcc cgtcgcctgg ggcttactcc cggggcaagg aatccgcttg
    28441 atgtcgaact caggctgacg cgtgagatac ctgaagaatc aaggccaaag gcacatcact
    28501 ggctgcttct ccacggccgt tatacctgca ctgcacgaaa gcccggatgc caggaatgcg
    28561 gactaaaaca gtggtgcgca tggtacgcat ctcattcggg aaaataagtt ggcgggaaag
    28621 tccgttataa ttcatgcata tatgagtaaa tttgttcagc cggacagcta ttcccctttt
    28681 aatgtataca ccagacatcc agatggaaaa gagaaaagag tcagtcaatg aaccggaaca
    28741 ctccgcggag tacctgacct ctctcagaca tcttgcagaa atccccgagc tggctttcaa
    28801 cagtacgaac cagctgatca ccctgagtaa cctcgagacc cagaagtaca tagaggttaa
    28861 ccaggctttt cttgacacca ccggttacag taaggaggag atcatcggca aaacctccga
    28921 tgacatccag ctatatgtcg atctcattca gagccgcaag tacatgaggc ttctctcgag
    28981 gctgaagaag gtgagtgacc tcgagatcaa actcaggatg cgttcaggtg atgagagaac
    29041 ttttctcttc agtgcccgca ccgttttcct gggcgatgag atattcctca tcacatacta
    29101 taatgacatc agtgcgctga acggaggctt tgcccgggct gagtcggaga agatcctgag
    29161 ggagattttc gattccatga gcagctatgc catcatcctc aggcgggagg gtgaaaacaa
    29221 acatttcatc atcgacttca actacagggc tgaggatgtg gagcagattg accgcaagga
    29281 tgtccttggc aaggagatcg gtgaaacacc gtttgccagg aacgaaacat tcctctcgct
    29341 cctcgagaac ctggaatcaa gccatcgtcc gtacaagata cccgtctccc cggacggcag
    29401 tgacgactca ggatattata tcggattatt gctttcttca ggagacatac ttattacctg
    29461 ggaggccggt aaagcacaga aggaaaggga gagcgaaatc cttaagcagg gaatcgtttt
    29521 cgagaccttc gccgaattgc tccctgagat gataatggag attgacaccg ggggacacgt
    29581 cactttcatg aacacccagg gaatgatgac actggggttt gacaacaacg accttgccag
    29641 cggtgtcaca ctgtcgtcgc tcttcatgcc cgacgatctt gacaggctga tgagggagct
    29701 ctccggcctg accggccccg gccagatcac ctttgatgag tatatcctgg taaacaagcg
    29761 gaaaaggaac gtatcggtac tttttcacgc cggtccgatc atcatcagga acagggtgac
    29821 cggttacagg tgcgtcctga ccgatgtaag caagcacaag gaatatgaac agaaactctc
    29881 gaacgagaag gctctcctgg agcacctgat tgacgctgct cccgaagcca ttgtgctgac
    29941 cgacttcgac gggcgtatca tccggatcaa ccagggtttt acccatctct tcggctatga
    30001 tgccgatgag gcgatgaaca ggatgattga tgacctcgtt gtcccggagg agcttcggga
    30061 tgaagcggta aagattgatg agacggccct ttccagcgga acgggctacc ttgaaacgat
    30121 aaggaaggac aagcagggaa acaggataaa tgtttcactg attgcctcgt cggtcatttc
    30181 aaacgacatg accatagcct tcctgggcat ctaccgtgat atctcgaggg agataaagag
    30241 cagactgatg caggagatcc agtataatat ctctactgtt gcgctgcagg actctgacat
    30301 ctttgacatc tatcctacca tagtaaagga gatcggcaag ctctggaacg tcaacaactt
    30361 tttcatagcg ctttacaaca gggaaaagga tactctcact ttccccttct tcgcagacga
    30421 gcgtgaccat ttccaggaga cggctgcaag gaacaccctc accggatggg ttataaggaa
    30481 caataaggct gtgctgctcg atgagaatga cattctcatg attgagaaga ccggcgccat
    30541 cagcctggtc ggatcacctt gcaaaatctg gatgggtgtg cccctcagga tggatgacga
    30601 agtgatagga gccatctgcc tgcaggacta caagaagatt gatgccttca ccaatgaaga
    30661 cctgcaggtg tttgagttca tcgccaacca gatagtgctt acattacagc gtcgcaggat
    30721 gcttgacaat ctcatcactg cccgcaagag ggccgaggaa gctgctttct ccaagcagca
    30781 gttcatgtct accatgagcc atgagatacg cactccgctg aatgaagtga tcggcattac
    30841 caacctgctg tggcagagtg atccgcgtga agaccagatg gactacataa atacgctgcg
    30901 cttctctgcc aaccacctcc tttcccttgt aaatgacatc cttgattaca acaagatgga
    30961 ggcaggcaag atcgtctttg aaaagaccga gtttgacctg acaagcatgc ttgaagacat
    31021 aaagcggtca tacagccaca gggccctcga gaagggtatt tacttcaccc tggagaagag
    31081 tcctgatctg ccggtgaccc taatcggtga ccccatcaga cttaaccaga tactttccaa
    31141 cctgctttca aacgcaatga aattcacatc gcagggagga gtgacgttga aggcttctgt
    31201 aaaagaccgc caggagggca gggcggtaat agagttctcg gtagctgata cgggcatcgg
    31261 gatagcccgc gagaagcagg aggagatctt tgagagctat gcacaggcat cgtctgacac
    31321 cacccgcaag tatggcggga cgggactggg cctggcaatc tgcaagaggc tggttgatct
    31381 ccagggaggc accatcggcg tgacaagcga gcctgacaat ggttcggttt tcacgttctc
    31441 cattgagtac agcatgccgg ctgcagagcc cagaacggca gagcgcggaa ccgcatcgga
    31501 cagcatgaag gctcttgtcg ggaagaagat tctcgttgcg gaagacaaca agatcaactt
    31561 cttcgttgcc aataaattcc ttgaaagctg gggtgttaag gtcactcacg tcgaaaacgg
    31621 ggcactggcg ctggaggaga tcagaaagca ggacttcgac ctcatactca tggaccttca
    31681 catgcctgtc atggacggca ttgaggctac gaggatcata agaagttcga ctgacaggaa
    31741 gataagccag ataccgattg tggctctcac cgctgcagtg atgtcagagg ctcatgacaa
    31801 gattgagaac ctggctatca atgactacgt gctgaagccg ttcaagccca aagatcttta
    31861 tgacaggata ctgaagaatt caagataggc gctgcaatgg cgcctttttt ttacctgata
    31921 cagtacctgg cgaggtaatc agttgcctcc ttcactccca gactccttgc cttgcggagg
    31981 taatagcagg catcatcact tctgccgctg tttatgagtg caatcccgag gttaaggttc
    32041 acattgccgt tctccggatc caggtcaagg ctcattgtat aatcctcggc agccctgtcc
    32101 caggtacgcg ctgcgagcca cgcgtcaccc cgcagcctga aggaagaggc ctcgccggga
    32161 tgcttgtcga cattgtcatt caggtagggc agcgcttcgt atattgcccc ctcttccgca
    32221 tatgtttttc cgatcagccc gagcgcctcg gtattgtcag gatcaatgga aatatacctc
    32281 tgcatctcct tcttcgcagc ctccctgtct ccggacctga tcagcatgcg tgcccttttg
    32341 agcagcaatg cagggtcagc aacatgggcg ctgatcatcc tgtcatatat ggctgccgca
    32401 gcgtaggcgc ttccctcccc ttcgcgggct tcagcaaggg tatagagcca tcctgccgga
    32461 gcatcactgt tcccggtgat ggtttcaagg atatccgctg cctcacggta cttgccgttg
    32521 ctcatgagga tgcaggcgtg acagtattcg gtaaccggtt tttcaggata gagtgatgcc
    32581 agctcggcgt atgcccccgc tgccatgtca ttgcgtcctg atttaaggta atagtctatc
    32641 tcccagcttt tcctctcatc acccctgtac cagtccttct tccagagcgc tctccactca
    32701 ggtgaggaag agacagacct gaatgaggga tcaagcatga tctccggctc gggcttcctc
    32761 agggcagact taaggtgggc ttccagcaat gagacagcag tctttgcatc gccaccggct
    32821 gcagcacacc tggcaaggcc atagagccct gatccctggc taaggttctc cgccctcatg
    32881 aaatcggctc ttgcctccct gatcatggat gccctgaggt agatgtcacc cctgactgaa
    32941 aggaggttgg cgtcgccatt tagttcatcc gttcctgcga gaagtgctgc agcctctcct
    33001 gcttctcccc tgccggcaag ggtgattgcc ctgaggaggg tctcgtaacc cgtctggcct
    33061 gacataatct gtgaggccag cagaacagcc aggctgaaca gtatcttttt cattgttttc
    33121 attttttacc ttcaataata ccgggccctg catccttttc ggcaaagaca tggataaaac
    33181 cgctcaggtg gaactgttcc agtccgctga tccttctctc gaacacctcg caatcatagt
    33241 agtaaacacc tgtcggcacc accctgccct tatatgttcc gtcccagttc agtctgggct
    33301 cctgggtact gaataccagc actccggctc tgttgaagag cctgaattca accttctcaa
    33361 ccagtgcgga ggtcttcgcc agcagccagt cattgaaatc gtcaccgttg ggtgtgaaga
    33421 cgttcggtat ctcatagaag ttgcatgagt caacacaaac catcgttgac aggggactct
    33481 cgttaccgat ctggtcaaat gttgagatgg catagcagcc tgcaacatag tcaggaagtg
    33541 agtgaacata ttttcttact tccctgtcat ttatggttgc cagcagcgta agatcctcct
    33601 ctgtggtctg tttgtaatag agtttgtatc ctgccacatc atcaagacag agcggatcct
    33661 caattgtcca cctgactgtg ttataaagac tgtcacattg tgatgacacc gtcagggccg
    33721 gcgtgcaggg cggttcattg tcaaccggca ctgcgcatgc acgctgtgag aggtttacca
    33781 ggttcttcgg tgttccggtg ccctgatagc cccccctgga aacaacataa tagcaatact
    33841 cggtgccgtt taccaggccg ccgtcagtaa aggtcagctg gtttgttgaa cccaccggct
    33901 cccaggtcga gccgttgtac ctgtaaaagt cataggaggt gttgatccac ggcacattcc
    33961 gggtcatctc aaagcgaacc tttctgtcgc cgggaagtgc ggtaaggaag agtgacgaag
    34021 ctatgcccgg atcacctatc aggaacctgt ttcccggggc attgttatac agttcaatct
    34081 tgtataccca ccccttgtca agcgtgttga tcagggtgtc aatgtaaacc gtgtcattca
    34141 ggtctgttgt cggaagggta tgtatcagct gccatgaggt gccggctatt ccttctgcac
    34201 ggtaaataag atattcatag ggtccgttgg caggtatggt gtcaaggcgg ccaggcttct
    34261 gccatgcgag ggtcagagat ccgttcacag catgggtgtt ccggaccgag acatttgtga
    34321 tcagggggat acccgtaatc agcgaggtga ctatttcatt ggatggtttg ctctccgtac
    34381 cgtttggata gacggccaca atcctgtatg catattcggt accggcctcc agggcatcgt
    34441 cagaatcggt gaagctgacg gttccgggtc ctccggcgta cccgaccttt acaaatcctg
    34501 ctgacggagg tatgccgtca tagcaagggt cggccggcag ggtggatgca ccctcgcgcc
    34561 tgtagatgct gaaccctgct acctggtcag tgacataccc tgcccaggaa agctgaacca
    34621 ccttgccctg cggcacggct gtcagcagca cgggggaggg tcccagcacc tttatactca
    34681 tgttgtcaat atcaaccagc tgcagctccc tgttggagtc ctctgccttg atgataatat
    34741 catatggctg gtgcctgacc gcattatggc atggtgtcca gctcagccgc gccgtagtat
    34801 ggccggcgat gcgtgacagt gtggtaaagg cagccggaca gtcagtggag gtaaagatgc
    34861 ccgaggttgc cagcagagtc actgaatcaa gatcggcatc ggtggcctgt atgtcgagtg
    34921 taacctgctg gccggcttca acgcacaggt cattaagctg tgatgtcaca ggaggtttgt
    34981 tgtctgtctc gtaaacctca atctgcatgt cgcggaccac gatcccgatc ttgatgcctg
    35041 cgcgccattc ctgtatttcc atggcgacgt tgtagatacc tatggcagtg ggggcatccc
    35101 agaccaggtc acctgatatg gaatcaacat aaaacttacg ggaagcggga ggcagcacat
    35161 aattctgaat cggtttgcca tcctcccttg tgcacacggt gagcttgtat gaaaggctgt
    35221 cgccgtcggg gtcaaaggct gcagggttgt ggatgaatat ctgccccagg gcagccttgt
    35281 cataaggcgg attgagcagc acgggagtgc tgttcctgcc aacggcagtg ttaacaatga
    35341 gtatggtaga gatggagaaa acgacgttta ccgagttggg aatgttttcc actccgaagt
    35401 tccggttcgg gtcctgaacc acaatcctgt aaaccccggg tccgggatag gtatgctccg
    35461 ccacgtagac gttcttcttg tagttgttcg gcagaatggt ctctgagatc cttgccacgt
    35521 tggtgataga gttatcaccc cattcgacgt caagtgccgg tctgtctgcc ttgctcagtg
    35581 tgtaggtgaa tgtggttacc gtcacctcga aggtgaggtc cgatatctgc ctgtatgtaa
    35641 tctcccctgc cctgttgtgt gttgcggtag cgctgaaagt cacagccagc aacaatgaca
    35701 gtgcataaaa aaatcgtctt ctcatctcac tccgtttcat ttcacaagat aactcttcaa
    35761 tgtatcggtt acagtgaacc tgtaaccctc ctctgtacct ccatagccaa agaggagcat
    35821 gtttgcctgg tcttcataca gccgggtcat gatgcggttc atcacggcaa cattcttcac
    35881 tcttttcctg ccctttgccc ggagggtgaa gcgatgttcc agaccgtcag caccggctga
    35941 taacagttcc atgtttacag gtttcccatc ctcaagtatc atgaccctgt cagaaagata
    36001 catgaaatac ctctcatcag tgctgccggt tatttcgccg ccgttgctca gaccaaggtt
    36061 catatcagct tcaaggtcat cactccagac cttaacaaag actgaccaaa ccctgttcac
    36121 gccgtcataa tctatacctg taagtgaaac atgtacagga tggatcagga gcaggagtgt
    36181 gagtgttatt gtcttcatgc cggcacggat ttaattgcag caaaaatcaa gccatcaggt
    36241 cttgttcggt cacccctgcc ctctccctga ggtagtcaac agtgcctacc tcaatgaacg
    36301 agaacccggt gcctctggag aagttgccgg ccagcaccac aactatgtcg ctgtctttca
    36361 tggtgtaatt cctcaggagc tcccttaccg agacatggat gaactcatca actgacattt
    36421 tcttttcaat gaagatgggg attacgccat atgaaagcgc cagttctctc atggtccggt
    36481 gtgaataaca gtgtgcgatg accgggttaa tacccctgaa agcagccatg tttctgatgg
    36541 cagtgccatt gacagtatca gccaggatag ctcttgcctt tattctgata gaggttttca
    36601 cagccacctt tgccaggtaa gctgaaaccg gtccgctcag tgtgccggct ggcatctcga
    36661 tgtactggtc cttttctttc tctgtctcgg aggcaatgcg agtcatggtt ctgaccgcct
    36721 ctacgggata ttttcctgca gcagtctctc cgctgagcat cagggcatcc gtctggctgt
    36781 agacagcgtt ggccacatca ctcacttcgg ctcttgtggg cctgggattc tgaatcatcg
    36841 agtgaagcat ctgcgttgct actatcacag gcttgcgctt ctcaatgcac ctggcgatga
    36901 tgtttcgctg gatgccgggg atccgctcaa agggaacctc gatggcaagg tcacccctgg
    36961 caaccatgat cccgtaagag tgatccatga tctcctcgag attatccacc ccctgctgat
    37021 tctctatctt ggagatgatc tttattgcac tggaccgtcg gtcaagcaga cgctgaaccg
    37081 caagcacatc ctcctttgac ctgacaaatg aatgagctat gaattcaatg tcaagctcaa
    37141 ccgccaggtc tatgaaaccg agatcacgtt cactcaccga aggcaggttt atccttacct
    37201 ggggaatgtt gacagtcttg cgtgaaccca gaataccgtc attttctatt cttccggtca
    37261 gggttttgcc ctcacggccg gtaatcttta tctcaagctc gccgtcatca ataaggatga
    37321 cagctccctc cgggacatac tttgcaatat cacagtagtt aacattgata acctcacggg
    37381 tggtgccggc tgccgaattg ccggagaccc tgactgtgtc gcccttgtgg aatctcaggg
    37441 gttcttcaca tgaggttgtt ctaatctcag gtcccttggt gtcaaggagt acggcaatat
    37501 tgtcagatat gctgcgtacg tttgccacaa tcttccttac gccgtcggta tcaagatggg
    37561 cagaattgac cctgacaaca tccattccgg cttcatggag cctgcgcagg aatgcgggct
    37621 cgcagttcct gtcggaaatg gttgcaacta tcttggttct cttcttgagc aggcttcttg
    37681 ccatagtcag cttatttttt cagaatttcg atgagagcct ctgccgcgag cctgtagccg
    37741 tcagttccca ttcccatgat agtgcccctg cagtaccgcc cggtgaggct tgtatgccgg
    37801 aattcctccc tggtcaggag gtttgagata tgaacctcta ttgccggtat ggtgattgcg
    37861 gcgacagcat cggccatggc cacggatgta tgggaatagc ctccggggtt gatgatgatt
    37921 ccgtctgcag tgcttccggc ttcctgtatc ctgttcacaa gctctccttc aatatttgac
    37981 tgcgagtacc tgaatgagac ctgcgggaaa cgttccttca gccggttgag aacggattca
    38041 aatccttcgg ttccgtaaat ggccggttct ctctttccga ggaggttgag atttggcccg
    38101 ttgataattt ctatcgtcat tttttctgcc acgctgatgg ttgtatctgt cagtacaaaa
    38161 ataaagaaat gaccggattt aagacggtta taagatataa ctttgggagc agcaacatga
    38221 gcctatgaca atatcgtgga cagatgccat caggggtttc ggcaggtatc tcaggatcga
    38281 gcgttcgctt tcgccgaaca gtgttgctgc atacctggct gatgtacgac ggttgcacga
    38341 ctgggtggaa tcacacaggc cggggacctg gccgtcagcg ctcacctaca gggacctgag
    38401 cgatttcgtg gcatttattg caaccaggga cgatgcagtg cggacccagg caagggtcat
    38461 ctcgggaatc aggtcattct gcaggtatat gctcattgaa aacctcgtca ccgatgatcc
    38521 gtctgcgctg ctggagctgc caaggacagg aatgaaactt ccggaggtgc tgtctgtgga
    38581 agagatcgac cggatggttg catcgataga cctgagcgta ccggcaggac acaggaacag
    38641 ggcaattatt gaaaccatgt acggatgcgg gttgagggta tccgaactgg tcaacatgcg
    38701 tctcactgac atccaccgtg gtgagggttt cgttaccgtc accgggaagg gtaacaagca
    38761 aaggttagtg ccgataggca ctgtaacgct aaaggagata gataattaca tggaacgccg
    38821 gatgatgatg ccggtaataa tcgacatcaa catcctgttt ctgaaccgtc ggggcaggaa
    38881 gctgacacgg gtgatggtgt tcaacattgt cagggattgt gctgccgcag ccgggataac
    38941 gaaaacaata agtccgcata ccctccgcca ctcctttgcc acccacctgg ttgagggagg
    39001 cgctgacctt cgtgccgtcc aggagatgct ggggcatgaa tccataacca ccacagagat
    39061 ctacacacat attgaccgca gctacctgcg tgacactgtt ttaatgtatc atcccagggc
    39121 ccgtcgccgg taatgctgca gcttccggac ccggaaacag gctgcttcaa caggcacaaa
    39181 tacattgtct cactgccagg aaaggcaacc actgcaaata ggaaaaagga tatttcttta
    39241 tttactcctt tttattacct ttgcggttcg aaaaaaaaga gattttaatc aataaccaga
    39301 tcgaatatgt caaaagttaa gcgagtttat tccttcggaa acaagacagc cgagggcagg
    39361 gcagatatga aaaatctgct tggcggcaaa ggagccaacc tggctgagat gtgtcttctt
    39421 ggccttccgg taccggcagg gttcacaatc accaccgagg tatgtactga gtattatgag
    39481 aacaacactc agcttccggc agaactgatg ccggaagtat gggaagccat gaaaaagacc
    39541 gagcaggcaa tgggtatgaa atacggtgac agggagaatg ccctccttgt ttcctgccgt
    39601 tcaggtgccc gcagctcgat gcccggcatg atggacactg ttctcaacat tggtctctgc
    39661 tccgagacta tccccggcct tataaaaaag accggcaacc cccgtttcgt atgggattca
    39721 taccgccgcc tgatcatgat gtacgcagac gtggttatgg agaaggctga aggaattgag
    39781 cctgctgacg gcaaaggaat ccgcaagcag cttgacgaga tgcttgacga attcaagcac
    39841 ggcagaggtt acaaatctga caccgatctt acatctgacg agctgaagaa cctggccgag
    39901 gaattcaaga agcgcatcaa aaagtcactc gggaaagagt tcccggatga cgctcgcgag
    39961 caactggaag gtggcatcaa ggccgttttc aagagctgga atggaaagaa ggctgtttca
    40021 taccgcagga ttgaaggaat tccggatgag tggggaacag cagttaacgt gcaggcaatg
    40081 gtgttcggaa acatgggtga ctcatctgcc accggtgttg cattcacgcg taacccggca
    40141 accggcgaga acctcttcta cggagagtgg cttgtaaacg cacagggcga agacgtggtt
    40201 gcaggtatcc gcaccccgaa cccgcttaac gacgacacaa agaacgaaca gaacaaacac
    40261 ctgcacagca tgcaggagtc aatgcccgga acatacaagg aactggttga catcaggaac
    40321 atccttgaga agtcgttcca tgacatgctt gacatcgagt tcaccatcca ggaaggcacc
    40381 ctgtatatgc ttcagtgccg tgccggcaag cgtaccggta ccgctgccct gaacatggca
    40441 atggatatgc ttcaggaagg tcttatcgat gagaagaccg ctgtgatgag ggttgatccc
    40501 gctcagctcg atgaactcct ccacccgatc tgtgacccgg cagccgagaa gaaggtttcc
    40561 ccgctggtca agggtctgcc cgcaggtccc ggtgctgccg taggaaaaat tgtcttcacc
    40621 gctgaggatg cagtggcatg gaaccgcaag ggtgagaagg ttatccttgt ccgcgaggag
    40681 acgaacccag aggatgttga agggatgcgt gcagctgaag gtattctcac cgcacgcggc
    40741 gggatgacct cgcacgctgc cctcgtggca cgcggctggg gcaagtgctg catcgttggt
    40801 gcaggcggcc tccatgttga cgtggcagga aagaaggcta aggtgaccgg atcagatgtg
    40861 gttctgaaag agggtgacat tatcacgctc aacggtacca ggggaaatgt ctatacaggc
    40921 tcactgaaac tgatggatgc ctcagagaat ccgagattcc agtcgttcat gaaactggtc
    40981 gacaagaacc gcaccatggg tgtccgcacc aattgcgaca atcccgatga tgcaaagatg
    41041 gcacgcgaat tcggagccga gggaatcgga ctcttccgta ccgaacatat gttctacggc
    41101 aagggttcag agcagccgct cttcatcctt cgcaaaatga tcctcagcga gaatgagact
    41161 gaacgcaggc aggcagtcaa cgagctcttc cccttcgtga agaaggatat gaaaggcacc
    41221 cttgaggcaa tgaacggact gcccgtgaca ttccgtctcc ttgatccgcc gctccatgag
    41281 tttgttcccc agggacacga aaagcaggct gaactggcaa aggctctcgg catatctgtt
    41341 gaagccgttg ccaaaagagg cgaagctctc catgagtcga acccgatgat gggtcaccgc
    41401 ggcgtccgcc tcggcgttac atatcccgaa gtctcagaga tgcagatcag ggcaatgttc
    41461 gaagctacgg cagaactgct taaggaaggc aagaatccgc tgcctgaact gatggttccc
    41521 gtaacatgcg acgtctccga actggatgtg actaaaaaga tctacgacag agtccttgcc
    41581 gaagtactga agaaattcaa cctcagcaag atagacaaca agtacggaac catgatcgag
    41641 atcccgcgcg ctacgctgct ggcagaccgc atggcaaaga ccgccgagtt cttctcattc
    41701 ggcaccaatg acctgacgca gatgaccttc ggcttcagcc gtgatgacat cggaggcttc
    41761 cttcatgatt atctcgacaa taaaatgctg gcagccgacc cgttccagac tattgatcag
    41821 gacggcgtag gacagctgat caccatggca tgcgtgaagg gccgctcaac ccgtccgaat
    41881 ctcaagatag gtatctgcgg cgagcagggt ggtgacccgg catcagttga gttctgcttc
    41941 aagaacgggc tcacctatgt aagctgttca ccattcaggg tacccatcgc caggctggca
    42001 gcagcacagg cagccatcag ggcatccagg aaataagctt tattcatgat aatgaggaaa
    42061 gggaggcaca ttcggggcct cctttttcgt ttagttggca gttggcagga aatatgtgcg
    42121 atttgcgatt tgcgatttgc gattgccgat ttttcccgag gggttaaagc attgtagtac
    42181 agtaccatat attgtctatt ggtcgtagac ccatcagcat tgtaaccctg ctgctatttc
    42241 aaaaccaccc acctcctgga aacatatctc caggcatcat cttcaccacc gctgattccc
    42301 agcctcaggg catatacccc tgcggcaacc gactgtgacg ggctgcaggg gatagaatgc
    42361 tctccggcgc ccagtgtccc ggtaaagata tttgcaacca aactgccatc agaagcaata
    42421 agactgatat ccacgcgaca ttcagaccgg agcgtgaatg acacccggcc ctcaccagct
    42481 accggattgg gaaaaacagt cagttctgtg aggtcttccg gaatcacctc aatgccggtg
    42541 ggggtgaaaa ccatggcatc atcgaagtac attgtcccgg acttaaggcc gccactgttc
    42601 tgcctgacca caatgctatg atactgccat tcaccgctgc cgccaattga gctgacggga
    42661 atggcaatca ggtcccaccc cgcccagttg atggttgcag ccggcactac ctgattgaca
    42721 gatccgggtg agtagaacca gtattccagc cggttctcgc taagatcgcc ataaacccac
    42781 attgcaaata cctggctggt attgttgcct ataaggggtt taccggtatc aaataccctg
    42841 catatgcctc cgttgtcgcc ggtgaagaga taatcgagcc ggccggccgg ggcattaacc
    42901 ttaaaggtat tatatgaagc agtgaaggtg gtcatcggat tgtcagtccc gacggtactg
    42961 ccgctcgtct cagggtccca gaaagcaccg atattgtcaa atgactcaat gacgctgcct
    43021 gactggtaag cctctgtacg agtggtaaag gttgtactca ccggagtgcc cgcggagatg
    43081 cctgagacat ccttcacaga agcgtcaatc tcaatccggt agatgcggtt ccgttccagc
    43141 tgtgaggcag gctcgaaggt gtagcttccc tttccgccct cacttttaaa ttgctcattc
    43201 tgtttcgtca acgggacaga ctggctgtcg agcagtctga ttccggctgt ggcagactcc
    43261 tggctcaggg gagcgtcaaa gagtattgtt atccttggaa agagagtgat gccggcagag
    43321 ccctgcgaag gccatatcct gtcaatcaca agcttcggtc tttttttcgt tacgaagctt
    43381 accaggtact cccctgcagg atgtacattc cacctggagg tggcagcggt cgtcagccgg
    43441 atgaggtaag gcgtcccgga cgcatatccg gttttcggtt taacagtcag gatcctgctt
    43501 ttttcatccc attcataggt aacgctcact tccggctcga acaccagggc ggactgaaca
    43561 gccagcatgt tcatgggaac attgaatctg aacgagaatg cctggttgaa cggcaggctg
    43621 tcagtcgttg aaggaagatg ttcaatgacc accggcaccc gtgcagtatc aaatgaagga
    43681 agcagatcag cgagtgttgt cattccggaa tttgcaacgg cattaacggt gtcattgtaa
    43741 aaatcagtca tgccgctggc gatgacctca tagttacccg gaggcagcga gtcaaacatg
    43801 aagaagccat tgttgaggtt gtcaatcatg acagaccgcc ctgatggctt cagggtgact
    43861 gttgctccgt taatgggtga gtttttgtct gtcgtttccg gaccataata ataaggtggt
    43921 gaggtcagct cgtcccttac ggttccagcg attatcccca gggattccgg attaacattg
    43981 tagaactgct gcagcgccct gagcattgcc catgcttcgt ggcggaggtg gttttcattc
    44041 cgaagcctcc atccctcggg tatgtaatca tggtatgatc cttccgacag caccccgggc
    44101 atggtgaggt ctctgagaac acccagtccc acctgatctc cccattcggg ataaaaggtc
    44161 cagtcacctt ttacccattg aaatgaatga gtccagtagt tgcttttctc atacagtttt
    44221 tcccacagga tctgtgccat tgatttggca gccgggaaaa caggagagtt gtcatagccc
    44281 ctgaagagta ccagcggctg gttccgggtg ccgtcgaatc cattgctgtg aattgaaaga
    44341 aaaatatcag catttgcctg gttggccaac tcggctatga ctgaaagttt aaggtcatca
    44401 gaggtgtaat ttgtggtcct tgacatgtag acagtggcct tccgggcttc cagcagatcg
    44461 cgcaggaaga gccccttggc aaggttcccc tccgactccc agaattcagt tgcaatcatg
    44521 tgcctgtcat cgccgtcatg ccctccatgg cccggattta taaagatctt cagtccgctg
    44581 aagtccgttc tctggccata caacaatacc ggcaggcaaa gaagagcagc cgtcagccaa
    44641 caccatttcc ccggtttcat ctgatccgta ctttcatcat gtatatctcc ccgttgtcag
    44701 tgcagaaggc aatgcgtctg ttgccggccg cgaacgggaa catctccgac ctgtcaggtg
    44761 tggaggtgag tattacccgt ttcttgtctc caagggaata ataaacaagt tcggatccgg
    44821 ttatcctgtg cccgtcatct ttatcatcca ttcctatcac aagcctgtca ttgaaccact
    44881 taggagcgtt gatcctgcca aggtcatgca ggatcctgcc gtcagtatca cagatgaatg
    44941 aactctgccc ccggtagtta tagaggatct tgctgccgtc aggagagagt gaggcccaga
    45001 tatagaatcc gtcaccgtta ggcatcatgg cttttctctc gctgccgcga aagagcaccg
    45061 gcatcagatc ctccgtcaga aggaaggttc cgttgttgcc ggatttcagg gctggacctg
    45121 cctgttcgac ctgcgttcct ttatccgatc tgacaaggac attgtttccc gcgactgcgg
    45181 gaggaagaac atccctcccc ttctccagga gcaactcttt cgtgcctgtg gcaatgtcat
    45241 aactgcttac agacgagaat ttgacgttat ctatgaaact gtcactcctg tacatgacct
    45301 tccggccgtc agcagtcact gcaggttcat acccggcgcc ggtatcaccg gttatcacct
    45361 gcaccctgcc tgtcttcgta tcaagcagac agagcccgtt gtaaccgctt cccgacacaa
    45421 gcaggagtcc tgacccgggc acaacacctg acagcagata agatccgctc tcttcgggtg
    45481 ataccttctt tacgctcctg accctgatat tttgcgcaca tatgctgatg gtaaggaata
    45541 atgccgataa aagaataact gttctcatgg tggttggtat tgccatctaa agatagtcaa
    45601 taggaagttc gggtgtcact atttcttcat agtctgcttc ttgactttta tcagtgtatt
    45661 tcgttactta atattattaa ctttgatgga catcaaaacc cccgcaccca tgatggcaga
    45721 agaaatcttg aagcaatacg gtattacaaa agtaaaaggg atagtctata atccttccta
    45781 tgaacgtctg tacgaggatg agctctcgcc cggcctgaag ggattcgaga agggtgtact
    45841 gacaaccacc ggtgcggtgg cggtcgatac cggaatattt acgggccgtt cgccaaagga
    45901 taagtacatt gtgcgtgaca gcactactga gaataccgtc tggtggaagt ctgagagtgc
    45961 tcctgtctct gacaacaagt cccttacgaa tgaagcatgg gaccactgca aaaagattgc
    46021 tgagtgtcag ctttccggca gggaactgta cgtcatggac tgcttcagcg gcgcaaacag
    46081 ggatacgagg ctctgcgtac gttttgtaat ggaggtggca tggcaggctc actttgtcaa
    46141 gaacatgttc attcgtccca ccgaagagga gctgaaagat ttcaagcctg attttgtggt
    46201 gttgatggct tcaaaggcgg tgaatcccga atatcagaag ttcggaatga attcggagaa
    46261 ctttgtgctg ttcaacctga ccgagaggat ggcgcttgta ggcggcacat ggtacggagg
    46321 cgaaatgaag aagggtatct tctcggtaat gaactactat ctcccgctga agggcattgc
    46381 cgcaatgcac tgttcggcca acgtcgggaa gaaaaacgat gtggccatct tcttcggcct
    46441 gtccggcacc ggcaagacca ccctctcaac cgatcccgag agggcgctaa tcggtgatga
    46501 tgagcatggc tgggatgatg agggcatctt caacttcgaa gggggttgct atgccaagac
    46561 catcaacctg agcaaggaga atgagcctga catttatcgc gccatcaggc gcgatgcact
    46621 gcttgaaaac gtggttgttg acgacaaggg cgagatcgac ttcgccagtg cagccaagac
    46681 ggaaaacacg cgggtctcct accccatata ccatatagac aatattgtga gacctgtctc
    46741 cagggccggg catgcgacca ccgtcatttt tctcacagca gatgctttcg gggtattgcc
    46801 cccggtcgcc aggctgactg aggagcagac gcagtattac ttcctcagcg gttataccgc
    46861 gaaggtggca ggtacggaaa gagggatcaa ggaacccatg ccctccttct cggcatgctt
    46921 cggggcggca tttctgctgc ttgacccgat catatacgca cgtgaactca cccggaagat
    46981 gagggcgcac aacgctgatg cgtggctggt caacaccgga tggatgggag gaccatacgg
    47041 gacaggcaag cggatagacc tcccttcaac ccgcaggatt attgacgcca tattgaataa
    47101 aaccatcaac gacgtaccgt acactacgat cccggtgttt aacctgagca ttccctcatc
    47161 attgcccggc atgccctctg acctgctcga tcccggcaaa aaatggcctg attatgaggc
    47221 atggctgaag gcagcaaggg agctcggtga aaaattcgtg aagaacttca gcaagttcac
    47281 atcgaacgtc gaaacggcaa aacttgtatc ttcgggacct aagccgtaag agatcgtaaa
    47341 tggtcatatc gttatcagtt attgcagtcg tgctggctta cctggcaggc tcaatcccca
    47401 gtgccgtctg ggcaggaaag ctatttcacg gaattgatgt cagggagcat ggcagcggca
    47461 acgccggagc cacaaacacc gtaagggtgc tcggatggaa gacgggcata cccgttctca
    47521 tatttgacct cttcaaggga tggctggctg taatgctgcc ggaattcctc gacgcggccc
    47581 ctgaaggctc cgagcctatg atggtgcttc agatcgtatg cggaatggca gcaatactgg
    47641 ggcacgtgtt ccctctcttt gccggttttc gcggaggcaa gggggttgca acaacattcg
    47701 gagtcctgct tgcactgcat cctctactga ctctgacatg cgccggcatc tttctgctgg
    47761 tgctgtttat cagcggttac gtctcactca gctccatgac tgccgtggcc atgtttccca
    47821 tattgctggt tacggtgttc cggacaccat ctaccctgct gataatgttc tcgatagtgg
    47881 tggcggtggc ggtgatcatc acccacgcca aaaacatcag gagactcctc aggggcgagg
    47941 agaagaagtt catcaggaag aaaaaggatt agcgacgcct cagtccggca attgcatctg
    48001 aagggttcct gactagcgct gccttagtcc ggcaattgca tcggcgaact tcctgcccag
    48061 gtctatataa ccttcactgt cataatgcca catatcactg taaccgtaat tgtcagttga
    48121 ggttaccagt gcggcattgc cgtcttccgc cacaaacgtc gcctgggctt ccctgacatc
    48181 atcaccccat gtccatacga tgctgtcggc atcgttgtgc gaatcagata tacggcctat
    48241 tgccaccggc attccaggag cggagaaaac tttccggatg ctgtcaacga gcagggccag
    48301 gttctcttta taaacagagg tggcgtaatc attattagcg tcactctctc cctgcatcca
    48361 taggattccg gagggaatca gccggtcctt tttcccatcg ccgtcaatat ctccggcagc
    48421 catcgcacct tttacagtgg cgaggaagtg gtcatactgg ttgattccgt ttcctccggt
    48481 gtatgaaggc agccatgccc cgaaggaacc cgctgctgtt gtgtcaattg aagttccacc
    48541 cctggaatat ttgactattg caatcttttc atcagggaag agctccttca ttctctcagc
    48601 aaaggtgagt tcaacgccaa aacggtctga ataggtgttg gcagtaccat cacttacgaa
    48661 gcctgcgccg tgtcccgggc ggagggtgct ccatagtccg cggccgtcaa cagcggtgtt
    48721 gtctccaaca gtgttcccct gatagatcat gacgccttca aactcgccgg tgagtccggc
    48781 aggaagctgg gccacataac cgtatccatc catatttgac tggcccccaa ggtaatataa
    48841 atgatattca cttccctttg ggttacacga ctgaagcgct gcaagaatta cagaaagcag
    48901 aacaaagatt atatttttca tagttaacag gaatgttttg atggcagata taagttctct
    48961 aaaagtagcc aaaatttcat tatttttatg gtctgacaca cataatcaat cactaaataa
    49021 gagaaagatg gaaggaataa aaggaacccg tacggaacag aatctcctga aggcatttgc
    49081 cggtgaatca caggcaaaaa accgttatga atttgctgcc aaggtggcaa aggaagaggg
    49141 ctatgagcag atagcagcca ttttccttga gacagcgctg caggagcagc agcatgccaa
    49201 gcgtttcttc ggttatcttg aaggaggcat ggttgagata accgctgctt atcccgcagg
    49261 caagacttta accaccggtg ataacctcga ggcagccgct gccggtgagc atgaggaatg
    49321 gtccgagctc tatccgatgt ttgcaaaggt agccgaggag gaaggattca aggaaattgc
    49381 cacctgcttc aaggctatct ctgccgccga gaaggtgcac gaggagcgtt acagaaagct
    49441 gctggagaac gtgatgggca acaaggtttt cgagcgtgag gagaaggtct tctggtactg
    49501 ccgcaagtgc ggttatgtac acttcggcac gaagccgctg aagaactgcc cggcatgcct
    49561 ccatccgttc aattacagcg aggtatacgc acagaactat tgatcttttg cctggtggta
    49621 tgaccgatgg agaagagatg ctcagcctga ttagaggcag gcagagtgag cggaaatatc
    49681 tcgacaagcc cgtcgagagg gaaaagatag aaaggatcac cggggcggga aggctgtcgc
    49741 cctcagcctg caatggccag ccatggcatt tcattgttgt ggatgagccg gcactgcgtg
    49801 atgaggtggc cgctgccacg gaatcggttg ttctgcgaat gaactcattc gtcagggagg
    49861 ctccggtgct gatagtggtg gtccgcgaaa aatcaaattt tagctcacgt gccggagatc
    49921 ttatcaaaca gaaggattat tccctgattg atatcgggat agcgacggca tcaattgttt
    49981 accaggctgc ggccgaagga cttgggactt gcattatcgg atgggttgat gataagcggg
    50041 tcagaaaggt tctgggcatt ccggcatcca gaaaggtaga gcttgtgatt tctgtgggat
    50101 atacagacaa ccgtctgagg gacaaatcgc gcaaaccgcc cgaagaaatt atcacgtata
    50161 acagatattg acaatttgtt cacgtaccgc cgggtctgag acccggcgca aatcatccca
    50221 atttctccaa cgcctccctt atccggcgga cagcctcggt gatgcggtcg tctgaggtgg
    50281 cgtatgagat cctgatgcag ttgtcggcgc cgaaggctgt cccgctgacc atggccaccc
    50341 tggcttccgc caggaggaag aaggtcaggt catcggcatt cttcatcacc ctgccgtcat
    50401 agctcctgcc gataaaattg gtgatgtcag gcatgacata aaatgcgccc tgcggcaccc
    50461 ttaccctgag gcccgggatc tcgcccagca gcctgcatat caggtccctg cggcggagga
    50521 atgcctcgcg catcctctcc ttcgaatcac tcttcgaggt gacggctgcc agggctgccc
    50581 tctgggatat cgaggagacc ccggaggtga actggccctg cagcttgttg caggccttcg
    50641 cgagccatag cggtgcgcca atataaccta ttctccatcc ggtcatggca tagccctttg
    50701 agacgccgtt gacagtcacc accctgtcgt aaatgctgct gaatgaagcc aggctgacat
    50761 gcttgccgga gaaggtgatg tgttcgtaaa tctcatctga tatgatgatc acctgcggat
    50821 acttctcaag aaccttcacc agggcagcca tctcctcgcg ggtgtagacc atacccgtgg
    50881 ggtttgaggg ggagttgaat atgagcagcc tcgtgcgcgg actgagggca gcttcgagct
    50941 gggccggagt gaccttgaag tcgctgtcga tcgatgtctg tactattacc ggtttgcctt
    51001 cggcgaaggt gatatgatca agatagctga cccagtatgg tgccagcaga atcacctcct
    51061 cacccgggtc aaccaccgaa agcacgacat tgataaggct gtgcttccct cctgctgaca
    51121 ctatgatctg gtcaggggta tagtcaagac cgttttcctc tttgaacttg cgggatattg
    51181 cctctcggag atcgttgtag ccgggcacgg gagggtagaa agagtagtta tcatctatgg
    51241 ctttctttgc ggcttccttg atatcatcag gggtgttgaa atcaggc
//
LOCUS       DAOPXU010000006        48888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_579_length_48888_cov_12.948785, whole genome shotgun sequence.
ACCESSION   DAOPXU010000006 DAOPXU010000000
VERSION     DAOPXU010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 48888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48888
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_579_length_48888_cov_12.948785"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PKK03_01185"
     CDS             <1..215
                     /locus_tag="PKK03_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83033.1"
                     /translation="YIAIPSVLAGLIGVWFTVDKWMQNFAVKVPMHWGLFVLCSLAIL
                     FSVALVAAVNYYRFANRNPVEALRYE"
     gene            complement(437..511)
                     /locus_tag="PKK03_01190"
     tRNA            complement(437..511)
                     /locus_tag="PKK03_01190"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(474..476),aa:Pro,seq:tgg)
     gene            630..1190
                     /locus_tag="PKK03_01195"
     CDS             630..1190
                     /locus_tag="PKK03_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNX83034.1"
                     /translation="MKKKLFTMAALMAFILAPALSQKSFYDFTVESIDGQQYSMADLK
                     GKKVMIVNTASKCGHTPQYKDLELLNKAYKQKLVIIGFPANNFMGQEPGSNEEIKQFC
                     DSRYGVTFPMMSKISVKGDDMHPLYRWLTSKSANGVLDSEVSWNFQKYIIDENGKLVG
                     FFKPAVKPLSDEVVNWVTKGQFIQAK"
     gene            1391..2413
                     /locus_tag="PKK03_01200"
     CDS             1391..2413
                     /locus_tag="PKK03_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNX83035.1"
                     /translation="MADLRTRYMGIELKNPIIAGASNMVTDIHKLQKLEQAGVAAIVY
                     KSLFEEQVQLENLELYELKTEYEHRHAEQVTLFPNSHTVPESPEDYLLHLKKAREAVS
                     VPFFASLNAINDEVWVEYAVKAAATGVDGLELNFYSLPDESGKDRETIEKKQVETLRK
                     VKEAVKIPVAVKLTPYYTNTLKFISELDTAGTDAVVVFNRLIQPDIDIYSEKHTMPYN
                     LSSSEDNKLPLRFTGMLHGNVRSSVCTSTGVYSANDVIKMILAGADAVQVVSTLYRNG
                     VEVVESMTGEIEKWMDGHGYKDLDSFRGKLSMKKSANKLPYTRAQYIDFMLNSGDIMK
                     KYRSLS"
     assembly_gap    2482..2581
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2700..3281)
                     /locus_tag="PKK03_01205"
     CDS             complement(2700..3281)
                     /locus_tag="PKK03_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX83036.1"
                     /translation="MARKSTRDMIIDVAAGLFATVGLRRTTMETIAAAAGRGRRTVYM
                     YFRNKAQIYDAVVEKEIRQITGPLSSLARSDGELGTVIHRYGEERMQLLGDLIMRNPL
                     LMKDFSQGHSRIEKLRDKLQKAEMQILIPLFRRHLPETEVSGEISPEDCAGLYLGMLR
                     GNDRLLTKTDGLSEAIRLSSISSWLLLRAISGS"
     gene            3368..5332
                     /locus_tag="PKK03_01210"
     CDS             3368..5332
                     /locus_tag="PKK03_01210"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNX83037.1"
                     /translation="MKRILLVFMCLLPFTAGGQVIVDKPLSARLTGYDIDVTLDPGKH
                     TVSGQMTAWWVNHSSMPVGEAMMHMYLNAFSSNKSTFASGGRWSASGDDGWGWVKIKS
                     MRDGSGNDLMGSCGYVAPDDGNENDKTVLRVVLPEPVAPGDTLVLKIDFISKLPSPIQ
                     RTGYDDDFHFVAQWFPKFGVYETAGMRQRETDGWNCHQFHPNSEFYSNHSVYNVSVTL
                     PSEFVTGSGGKLIEEIDLGGGLKKEVWRAEDIVDFAWTAWPGYKVVTRKWNNVDITFL
                     TSKMGLSKAENHLDAVVYALEYLAARVGPYPWPHLTFVDPPLSGSGAGGMEYTTLFTT
                     MGGGAVPTFMKMPEMVTIHEFGHAYFMGILASNEFEEPWLDEGVNSYWEQRIVDHYYG
                     GGYGLVRLPFLKMSDTDQGRLSYISSSNRTIASNDLPSWMYPHGTYSMMSYNKAATWL
                     HTLQGIIGDETMDNVFREYYRRWAFRHPSGRDFISVVNDVVKKEHGDRFGENMNWFFD
                     QVLYGKGECDYRVSGITSFRIRSYSGVVDGDSVTYARSDRKSDTLYISRVSVERLGEV
                     MLPVEILVRFDNGEEVLEKWDGKTSYKDYEYTGARKVVWAKLDPYDRIDMDVNRINNS
                     WTLEPNRTATRRMLNKFVLLMQMMISIFTL"
     gene            5343..6203
                     /locus_tag="PKK03_01215"
     CDS             5343..6203
                     /locus_tag="PKK03_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83038.1"
                     /translation="MSLFKTLKNGAAAAASSYKLILLIWVTTLVLTLAVGFPLRSFLN
                     MMFGSSLAIERLNDGFDLGIAGDLGKSLGGLMASVSAGTMLISLAGFFIMTFFAGGLF
                     RRFSLAWGGLKVSDFLRASAGNFIPFLKIALLMILIIGAYTFVLIGIPVIAGLIASGS
                     PRQNGKFFYLFYVIWALGLPVWLFVADASRRWIAATGSRKVFRALGAGFRAIRERFWH
                     SYLTVLAVIIFNVIFIAALMWFAATSTPEKGIMVFLFFIATQALFIIRLFMKAWRYAS
                     VCEAVQQVKS"
     gene            6257..8614
                     /locus_tag="PKK03_01220"
     CDS             6257..8614
                     /locus_tag="PKK03_01220"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83039.1"
                     /translation="MKRCTSGIVPAILLVILLFVMPFRLSAQAKQPVLKGTGRVKMFA
                     AQAGLRASSPYKDLHWQYIGPTNISGRCTDVEAVSPRGGQYTIWVGSATGGVWKSVNE
                     GTTFESVFDEMPTASIGDIAIDPKNPDVVWVGTGEANIFRSSNAGCGVFKTTDGGKTW
                     DLMGLENTHTIGRIRVNPGNTDIVYVASPGHEWTPNEERGLYKTADGGKNWTKILSIN
                     QNTGVCDIVLDPRDPNTLYATTWERTRLKWNDPRTFADTRNCGVWKSTDGGKTWKEIN
                     AGLPAPQHRGRIGVDISLSNPDVLYLLLDNYEIAYKAKPGELDSYGRQKQDVIKGATV
                     YRSGDAGASWKQVSGLTGETRVYMEYHSGTYGWVFGQIRVDPKDENTIFTLGIELHKS
                     VDGGATFRTLDGPHADHHGLWIDPNNPNYLLNVQDGGLTISYDKGATWKYPIAELPLA
                     QFYNIAYDFSVPFRVFGSIQDHHSFYGGVDLSRGRDRVRPVEFRNTLGAEGSSHAVDP
                     RDNNTIYSSTFYGALARAEIDNYPASRKDLLPVRYADEPRLRGQWVAPTLLSPHNPDI
                     VYHGMQYVMMSRDKGNTWEFISPDLTFNDPKKQGDISYQTIQTLDESPMRAGLLYAGT
                     DDGRIWRTKDGGKIWTELRPKNVPVRWVSRVVASKYDLGTVYMTQTGRRDDDFQVYVW
                     KSTDFGDTWTDISGNIPVGPVNVIREDPVRKDLLYAGTDAGVYVSKDGGARWEVLGDL
                     PFAYVHDLQIHPRDNMIIIATHGRGMFVLDANPVNEKDKMRKRWY"
     gene            8723..9295
                     /locus_tag="PKK03_01225"
     CDS             8723..9295
                     /locus_tag="PKK03_01225"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HNX83040.1"
                     /translation="MALTTGTNGNGERVKLIRKIHRLTASFLSVALIIVAVSGILLGW
                     KKNSNGYLHPVSHEGTTSDFRQWLPLDSLNALAVRALHATGRKGLSEELDRIDIRPDK
                     GMVKFVFAHHYHGIQLDGATGEVLYVENRRSDLVEDIHDMSVIDRLFGIDGELFKLIY
                     TTFTGLSLLVFTATGIWLRYFSRRGKEKKR"
     gene            complement(9346..10938)
                     /locus_tag="PKK03_01230"
     CDS             complement(9346..10938)
                     /locus_tag="PKK03_01230"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNX83041.1"
                     /translation="MKKTGIISHLFLLMAAVCLLSSCKNETVITFRSLLREMTSAETL
                     TRYPTPDYRLVQFSSYDRRSIHPDSAGWYANNDYTHFIREETTGGRCEYVMLEADGPG
                     AIVRWWMTFGDEDALNSYIRVYLDGRTEPVIEGMAPELVGGGVLAPAPLSTSVSPLTE
                     PQRRGYNLYLPIPFAEKCIITLENDSVLITPQRRKPSIYYNIDVRLYEEGIRVASVKS
                     ETFRDDTLAIAECAADLDRAGSNQTTGKEQRSSDGMFPPGTECRISVTGKNLAVSNLS
                     LSLSGSDTAALLKGTIIRMWFDGHSTVEVPAGNFFGTGYTYNSCHTRFTAAETDGTLS
                     SSWLMPFRDSCRISILNSTDDTIAVKTLVTLAPYRWNENSMHFGASWKEYPGFETAGS
                     ENTGGTGLHTDVNIAGLRGKGLYAGDAVLVFNTADAWWGEGDEKIYTDGETFPSSFGT
                     GTEDYYGYAWCRPELFDHPLIAQPAGYGNFHPGMSVNMRYRILDAIPFRESLRADIEM
                     WHWVKTTIDFSVTAWFYRLPEF"
     gene            11023..11190
                     /locus_tag="PKK03_01235"
     CDS             11023..11190
                     /locus_tag="PKK03_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83042.1"
                     /translation="MNAILYAMRAIVVIHGTIAYCLTLLPIAYWYCLPDPPAGGYGIE
                     PPPTSESQTAY"
     gene            11293..12060
                     /locus_tag="PKK03_01240"
     CDS             11293..12060
                     /locus_tag="PKK03_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNX83043.1"
                     /translation="MAISELMEDLYSRLLQDIRFEEGLKACMNCGICTGICPAAEYYN
                     YDPRRVVDLVQTRNNDVIRELLKSETIWYCGQCMSCKTRCPRGNTPGMVIMALRKLSQ
                     ELGYFTASEKGRQQFAIKRTVGDNVLKHGYCVVSYDVDPEAHPEQGPAWEHYYNHMDD
                     SLARLGGELNGREGPTRLIPDDVLDELKSIFKVTGGQDLYDAIEEASEKKAEEMGMEF
                     KKEGRDFEYFRHVVSADSGCHQNEPEDIRKDVYETGR"
     gene            12044..13105
                     /locus_tag="PKK03_01245"
     CDS             12044..13105
                     /locus_tag="PKK03_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNX83044.1"
                     /translation="MKPEGKKRIWAEYQKEIPDDHFFYVRSCIRQTFFAGSEQYFLKI
                     LRDDLGKDVFEDPRHTTCTGIGYHGDVVPFETIQAVVARHFALATEAGYRHIAISCVT
                     SFGIYSEILETWKHFPETLERTREDLYKATGRTFEVPETISHTSDIIYKFRDQIARKA
                     KYNLVDINTGKPLKVVEHIGCHYSKMFPKYGVGGAEFPHVLVGMVEAWGGDIIDYPER
                     RHCCGFGFRQYLVQANRGYSLFASRKKFESMEPYRPDMIIANCPGCTMFMDRWQYTIR
                     EIDGITYGADGQGIPVLTYEEVAGLILGYNPWELGLQYHNVQSLPLLRRMGIKADPSE
                     RFLAKDGSRLPEPANLING"
     gene            13102..14133
                     /locus_tag="PKK03_01250"
     CDS             13102..14133
                     /locus_tag="PKK03_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX83045.1"
                     /translation="MKTTSAHIVVAGGGVAGMEAAGTLAEMGYRVTMIEKDDEPGGHL
                     LQWHALFPDRRPADEVRNYLRKHIEQRNLSIITGTTVEKIRHHGSGVLVSLSGGTEEK
                     ADAVIVATGFDLFDARRKEEYGYGIYDNVITSAELEGMFNRGRVAKADGSVPARIGLI
                     HCVGSRDEKVNNNHCSKVCCVTAVKQAIEIRKMLPSCEVFCFYMDMRMYGPYYEELYR
                     ESQEFHNVNFIRGKVSEASVNINNRLVIKVEDTLAGRPLKMELDIMVLMVGMEMAGAG
                     MKLAESSGMKRGINRFLETADHHYGNNLSGIKGIFYAGTCTAPMNITDTISHARAAVA
                     ETVRYLNSR"
     gene            14130..14489
                     /locus_tag="PKK03_01255"
     CDS             14130..14489
                     /locus_tag="PKK03_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNX83046.1"
                     /translation="MSIAQSNKFGFTINRSRLIDYDSSDRKVREFITLKEPSFRLCFA
                     CGGCTATCTAGQHTSFNLRRMNTYIRRGEIEPLREEVKRCMLCGKCLLVCPRGVNTRN
                     VVAAAADAIGKLERNEL"
     gene            14479..16377
                     /locus_tag="PKK03_01260"
     CDS             14479..16377
                     /locus_tag="PKK03_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNX83047.1"
                     /translation="MNSELFTLPFNLGLYFILIYSVVRSTVWFRNLSRSDKLRLQRGF
                     FGRPFMDSLREISMESLLHLKIFRKNPVLGYMHMSLAFGWFLLIVFGTIEADFFGEKH
                     LNPPSHAIFFRWFNPDHGAEGFAGIYSFWMDLLLAFILSGLLLAIIKRFTPRLMGMKK
                     TTKHTWTDRIALTSLWLIFPSRLLAESFTCGVHGSGSFLTGNLGNALASFLPAADIAP
                     WFWWLYSLSLGTFFFLLPVSRYFHIPVELFLIFMRNSGISTGDRHSSYTEVQTHACSS
                     CGVCISTCQMSYAAGIHDIQSAYFIKGLRNDRDIAGITANCLMCGRCEEVCPVGIELG
                     PMRLIERRQGERSDRTLKLQEKYSLMRRRSTLERRPTQSYRYLVREEPEKADVLFFAG
                     CMTHLTPSVIVAMKKIMDSAGVRYRFMDENGGVCCGRPLMLAGRDREARELINHNSDV
                     IWKSGAQILVTSCPICYKVFRESYYLEARVMHHSEFINLLIEEGSLRLGYLHKKVVYH
                     DPCELGRGSGIYEEPRAVISYVAELQSSEQERNMSLCCGGSLGNLTLSSGKRAMIAAD
                     AARTLTLGLPDELITACPLCKKTFVQATETKVSDIAELVAAALPAPRRENAKKRPARR
                     HSRATSNG"
     gene            16517..17068
                     /locus_tag="PKK03_01265"
     CDS             16517..17068
                     /locus_tag="PKK03_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83048.1"
                     /translation="MKRIFFAAVVLGVAVFSGLTSCKNDAGLNGQEGKNRKGATVEGE
                     AVVLSIGEAELIEVDDNPQYNTAEWLFKVEKPGRWDVWLSSLTVDTTQLHFAENVIIT
                     AGETKLEKKPVSDRIVLGDKSITEPWYRADSHMGSVFFSKPGEYQVQVISDKVEPHSS
                     DLSQLSIAKHTLINSVILKPKVN"
     gene            complement(17101..20802)
                     /gene="purL"
                     /locus_tag="PKK03_01270"
                     /gene_synonym="purI"
     CDS             complement(17101..20802)
                     /gene="purL"
                     /locus_tag="PKK03_01270"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNX83049.1"
                     /translation="MTSGTVLFFRTSSGNVIAVRAEGQLTRDDTAKLSWLFGNASLME
                     AETVSGTFIGPRREMITPWSTNAVEITQNMAVSGISRIEEYFPAAGENPAHDHMLQRI
                     YHGLDQDIFTVHHEPEPVREIDDIAAYNAQEGLALSAEEIRYLENLSRNLGRKLTDSE
                     VFGFSQVNSEHCRHKIFNGTFIIDGEERKSSLFDLIKRTTKENRNHVVSAYKDNCAFL
                     DGPVTEQFAPATQDKPDYFCAKYIQSVLTLKAETHNFPTTVEPFNGASTGTGGEIRDR
                     IAGGKGSLPIAGTAVYMTSYPRPDGPREWEKYSEPRKWLYQTPEEILIKASNGASDFG
                     NKFGQPLICGSVLTFEHFTDMKRYGFDKVIMLAGGIGLGKKSDSTKNTPEKGDLIVLL
                     GGDNYRIGMGGGAVSSVDTGAYDSAIELNAVQRANPEMQKRVYNALRAMAESDHNPVI
                     SLHDHGAGGHLNCLSELVESTGGTIDISKLPVGDPTLSAREIIGNESQERMGLVIRSS
                     DVDQLTSVARRERAPIYVIGEVTGDHQFTFLNPSGGNRPIDLQLADFFGKPPRTVMND
                     TTMKSLTGDLIYEPSDPARYLDAVLQLEEVACKDWLTNKVDRSVTGRIAVQQCAGELQ
                     LPLNDLGAIALDYRGMNGMATSLGHAPAAGLVSAAAGSVLSIAEALTNIVWAPMPDGL
                     KSVSLSANWMWPCKNPGEDARLYEAVQAASNFAVALGINIPTGKDSLSMTQKYPDGTV
                     YSPGTVIITAAAGVEDIRRITGPVIRNDESASLFWIDMSGMRHEPGGSAFSQTIGSLG
                     TVAPGVADAEYFAATFETLQLMIKDDRIIAGHDISAGGMIVTLLEMCFANREGGMTLN
                     LTEIGETDTVRLLYSQNPGVIIQVRDEAETEEFLYERGVRFASIGHPVRERKLSIVNG
                     DNHFEFDIDRCRSLWYRSSYLLDRRQCGPVLARERYDNHGEKAIKFDLKNFKGSFRSQ
                     GITPDRKATTGVRAAIIREKGVNGDREMAYALWLAGFDVRDVHMTDLISGRETLQDLS
                     MIVFVGGFSNSDVLGSAKGWAGAFRYNEKARIALEDFYMREDTLSLGVCNGCQLMAEL
                     GLIVPEHKEMPRLEHNASHKFESDFLGVKITDGKSVMFTGLEGMELGIWVAHGEGRFS
                     LPLPEKNYNVVMKYSRSHYPANPNGSMYDVAGICSADGRHLAMMPHLERSFMPWQCAY
                     YPADRRNEDITPWMQAFVNARKWVEGKKK"
     gene            complement(20949..21920)
                     /locus_tag="PKK03_01275"
     CDS             complement(20949..21920)
                     /locus_tag="PKK03_01275"
                     /inference="COORDINATES: protein motif:HMM:NF025608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HNX83050.1"
                     /translation="MLTKIRSLFNPSMYQGWGNTRRYFEGWYFKVVNAPETAAFAFIP
                     GISMNEAGNKQAFVQVLDGKKKTAEYFPFESTAFVPSEKNFMVTVGNNFFSASRMRLE
                     LPSVSGELTFTGTREWPNPWYAPGIMGPYTFAPFMECNHGIVSMDHGISGSLVINGEE
                     IDFTGGRGYIEKDWGHSFPSAYIWMQSNHFAANGISFKASVARIPWLTGNFTGFISGL
                     MIDGRLYPFTEYGGSKIVSLQVTRQKVEITFRNRKHTLSVSAPRDTATPLAAPIKGFM
                     GGRIEESMTSAISLMLSETSTGRVIFSGEGRNGSIEISGDPDTLMLH"
     gene            complement(22012..23328)
                     /locus_tag="PKK03_01280"
     CDS             complement(22012..23328)
                     /locus_tag="PKK03_01280"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNX83051.1"
                     /translation="MRQKDAIAVFWFRRDLRLEDNVGLYHALRSGHPVLPVFILDTEI
                     LDRLEEKADRRVAFILAALGEINKKLASAGSSLKVLHRTPVEAFRKILDEYDVKAVFT
                     NRDYEPYATARDEKISLLLKNSAIPFHSFKDQVILEKKEVVKSDGTPYTVFTPYSRAW
                     KKQLTIQDTESFFSEKHAGNFLKLPAARIPGPEEISFTNTDTVSPVPAINQEIIRNYH
                     LTRDIPSLEGTTRLGVHLRFGTVSIRQLVRTALDLNETWLNELIWREFFMSIIWHFPH
                     VTDSSFKARYDAIQWRNNEEEFDRWCNGMTGYPIVDAGMRELNTTGFMHNRVRMITAS
                     FLTKHLLTDWRWGEAYFAGKLLDYELSSNNGNWQWAAGTGCDAAPYFRVFNPAEQTRK
                     FDPQMRYINRWVPELGSPSYPSPMIDHAMARQRALATYASGLGRGD"
     gene            complement(23336..23920)
                     /locus_tag="PKK03_01285"
     CDS             complement(23336..23920)
                     /locus_tag="PKK03_01285"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNX83052.1"
                     /translation="MNAELITAVAIGIGLAASAGFRVFVPMLVAAIAAKTGILPLNDS
                     FQWLSSWPSIAILGTATVVEILAYYIPVVDNLLDTVSTPLAIGAGTLLLTSVLPIDSE
                     LMKWITGAAVGGGSAAVVQSGSALTRLTSTKLTAGLGNPVVATVENVAATGTSILSLV
                     IPFFILALFLLLTILIFTSVRKRLRRSHNENLRA"
     gene            complement(23936..27157)
                     /locus_tag="PKK03_01290"
     CDS             complement(23936..27157)
                     /locus_tag="PKK03_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HNX83053.1"
                     /translation="MNKYLVILLMVFPPLAASGQANPFKIKEKKNGTPVIDLPVIPRQ
                     PVSPGRTVAQSGTPQIRTLVQPVLSGHKERSTQIIRHRGRPVFIEKESRETKASPEDR
                     FYMFLDETKALTGVENPGESFRITGISTDRLGITHIRTVQQFRGVDIYGSEATVHLDG
                     ARERFTGAFHRPDRNTKTTPSVSAATAVRKATEDVSAETVYRKLSQKEQEILDYSAPS
                     TSLVLYSTGEDKYRLAWAVTIRPNFIEEWKYFIDAAGGEIIHKFNSTCSDGAVPAQAY
                     DLNNILQTISVYLESGTYYLINISEHMFNGATDEGVIMTLDANNTSTTDLDYSYITSS
                     NNAWPVRSAVSAHNHAALAYRYFWNTFGRNSINGRGGDIISLVNVAEDDGTSMENAFW
                     NGKAVFYGNGGTSFYSLAGALDVAAHELGHGVVSNTANLEYYGQSGAINETYADIFGS
                     MVDRDDWRIGEDITKTSYSPSGALRNMADPHNGGTSLSHPYWQPKSVSEMYTGSEDNG
                     GVHINSGIGNHAYYLYATAVTKEKAEQVFYKALTDYLTMTSKFIDFRIAVVQAATDLY
                     GASSTEVAKAEEAFTAVGIQQEEQVDKIQDYTVNAGQEYLLNYNTSSSFSGTLYRTSG
                     TEDAPLTSTEMKGKVSVTDDGSLAVFVSTDSKLRGLSPDNASDPGEFMISGQQVWDNV
                     AISKDGKRIAAISIYADTSIYLFDISGAVVRDTVFKLYNPTTSHYNTTAGGVLYADAV
                     EFDITGQYLVYDSYNELTSSTGEDIGYWDIGIINVWDNNENDWGDGTISKLYGSLPED
                     ISIGNPVFSKNSPWIIAFDYIDAANEQYAILAADLNAGLDNLITMNNTLGYPSFSKND
                     DRIAFTKADGLSQSIYTIGLGADKISPAGTESGYIQDARWPVYFATGERALSLPPVAD
                     FTVDIKTGNAPLRVKFFDLSANQPSSWAWTFEGGTPASSTEQNPVISYNTPGTFRVSL
                     AASNNQGSNTSTRNGYIIVSAASAVEETGQDAIMVYPNPVSDILHIISETEYSARLFD
                     LNGRLVHSGTNDQIINMTRMGKGVYLLELTAGGKTTRLKIVRE"
     gene            complement(27154..27564)
                     /locus_tag="PKK03_01295"
     CDS             complement(27154..27564)
                     /locus_tag="PKK03_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83054.1"
                     /translation="MYRILIFCMIMATSCSSPEKLLNSNRGDRIFFGNKGGFTNMSTD
                     YVLFENGSICRLKNDSVIRTGKISREEVREIEVSLDAMNFMSVETDEPGNMTWYVSVV
                     RGDSRNAVQWSDHDRNPQLKELYVRLMNLVKEQQ"
     gene            complement(27675..28922)
                     /locus_tag="PKK03_01300"
     CDS             complement(27675..28922)
                     /locus_tag="PKK03_01300"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNX83055.1"
                     /translation="MKKAVLSIILAISLQSMNGQMQQAIERIAGEYGLMGLSVVAVCD
                     GKITGEWYTGLRDYDRNLPVDENTKYRIASISKFVMTTAMMKLCDQKRLDLDADAGDY
                     LGFRLRNPNHPEKPVTVRMLLLHTGSLNEGSGYDPFLMATYNNVGNPPSFSELFAPGG
                     KYYTDDMWRKEAPGEYYTYCNANFGLAGTIIERITGQRFEEYIQQTLFIPLGISGSYI
                     AEGVTDINNLAVIYGREDGKWIPGTDDYKGMMSSPRDFSGYVTGTNGAVFSPQGGLRI
                     SAAELARIMILHMNKGKYEGKRIISGKSIRLMHRAQFITTPANSDNEGVRAGNRGLSV
                     QILTGSASGSILPEGSHFLGHSGSAYGLVSDFYFDPKKKYGFIFITNGIAGGPEAGSS
                     GSFYNFEEALIRALHENSEMPCK"
     gene            complement(28936..30414)
                     /locus_tag="PKK03_01305"
     CDS             complement(28936..30414)
                     /locus_tag="PKK03_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HNX83056.1"
                     /translation="MPRRITLAAVIAVLTCLPVAAQEPDSLFIGIHQAESQYYSGVFD
                     TASLRSIASPGTPVTLKSRKEGEPAFLVYGWHPYWASDTAFLHYDYSVLTHIAYFSYE
                     VDTETGSYTTLRGWDITPVIDYAHQQGVKVMLTVTNFGSDRNIALLTDTVKQRHLINT
                     LIYQLKMRNGDGVNFDFESVPASMKANMVSFCRRAVRGIKAELPQAEISLATPAVNWS
                     DAWDLNALADICDYLIMMGYNYYWSGSTTAGPVAPLRGENYNITRSIQEDYLDAGVPA
                     SRLLLGMPWYGYDWPVSSSFRKAGTTGTGSSRTYIATIPMVNANGKAFDNDTGVPWMS
                     YQNDTRWRQMWFEDSLSLLLKTDFAKKKNLAGVGIWALSYEAGRHELWNGMKAVIEGT
                     VSSDGSPAMPGTGGSEILSLNPNPVKDKALVSFYLSRPCAMSIAIYDSGGRLVEVTEQ
                     GSLPPGMMTSEIDAEKLAPGIYFCVLYTGEGRSVRRFSVTGN"
     gene            complement(30435..31160)
                     /locus_tag="PKK03_01310"
     CDS             complement(30435..31160)
                     /locus_tag="PKK03_01310"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HNX83057.1"
                     /translation="MTNDVFSKIGSGLTLSQKIERRIEEAIRQKKLLPGSKLPTEKEL
                     CTQFAVSRTALREALRRLSARGLIDIRKGSGMYISELKIEDAINSLHLFYDLRFNSDL
                     ILQIIEVRRLFEPEVARLAARNRTDEDIRTLQKNQHELEKCNPDNTQLEVDLINRFHM
                     NLSKATGNPIVIISLEPVYSLLPRMRNLIYGNIEGEKDYTMKYQSELLEALKEKDSQK
                     AYEVSVVLLERNMEIYKKYFKTN"
     gene            complement(31162..32004)
                     /gene="murQ"
                     /locus_tag="PKK03_01315"
     CDS             complement(31162..32004)
                     /gene="murQ"
                     /locus_tag="PKK03_01315"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HNX83058.1"
                     /translation="MIRQAEKKQDLSITESPSNFEDLDKMTVNELLTNINHEDAKVHV
                     AVKNAIPDIENLVNQIHVRMQKGGRLFYLGAGTSGRLGVLDASEIPPTFGVPDTVVIG
                     LIAGGETALRKAVESAEDDTTKAWSELEIFNINTKDSVIGIAASGTTPYVIGGLQKAR
                     ENGILTGCITCNPKGPIVDVAEYPIVVVVGPEFVTGSTRLKAGTAQKMVLNMITTTLM
                     IKLGRVKGNKMVNMQLTNKKLIERGTRMIVEELNIQREAARILLMEQGSVKKALEFYR
                     ENYR"
     gene            complement(32009..32857)
                     /locus_tag="PKK03_01320"
     CDS             complement(32009..32857)
                     /locus_tag="PKK03_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNX83059.1"
                     /translation="MILIADSGSTKTEWREVSDGVAGKSYISTGINPFFVTGEEMIRL
                     FGKELPELKGAGVSRIFFYGTGVSNASKADIVRAALSSFFGTDKLFIGSDLLGAARSL
                     CQNEPGIACIIGTGSNSCYYNGNEIVANVSPLGYILGDEGGGAVIGRKLVAGVLKKQL
                     PGIVIENFFRAYPFTPAEILDNVYNMPFPNRFLGQFTRFISDNIHVPELQAMITSSFD
                     EFIVRNVLSYPEARRYPVHFTGSIAYHFRPFLEELLLKHRLQPGLFTLTPMENLVKYH
                     IQNSDK"
     gene            33100..36078
                     /locus_tag="PKK03_01325"
     CDS             33100..36078
                     /locus_tag="PKK03_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthan lyase"
                     /protein_id="HNX83060.1"
                     /translation="MAILIAGQQSAAQKAGRAGRLAVSKMEKWVSPVGEGTLPERFVI
                     DSLKVDEEKREINIWFPVAASYNPIREETVTGLNSSVRSALGRKFAGYRINMISNGLS
                     LESLVPNYFRNVMPDDGDRIMPAAEAPAGLIRRVNAVSPSRGLEGRHIALWHSHGYYF
                     DMPLDRWEWQRAKLFGSVEDISVMAYVLPYLTPMLENAGAVVLLPRERDIQVNEVIVD
                     NDISTGASGFVLQIPGEPEKAGKGFLLKDTLFNGDNPFMAGTSLKISSGSALYVPEIP
                     ERGWYGVTVSYPRVPGYKGKAMVRVTHTGGVSEFIVDQSIGGGTWLWLGSFLFGAGAD
                     PLKGSVTISGMDGSAALLDAVRFGGGMGNVARRPAESMISNQWSLNTGSQQETADSAP
                     GPREYSWKLSGKPRFLEGARYWLQYAGMPDSLVYSPNKGRNDYNDDYMSRAEWVNYLL
                     RRPDTTASGGPGIPVDLSFAFHTDAGVTPDDSIIGTLGIYSTITNGGLFPDGTSRLAS
                     RDLTDIVQTQVVEDIRVLFNPEWTRRAMWDRSYYEARKPDVPAMLLELLSHQNLADQR
                     YGFDPRFRFHVSRAVYKGILRYLADTGGREYVVQPLPVSHLAIEPVEGRRVTIRWQPV
                     SDPLESTAAPVSYRVYMRSGDDGFDNGTPVSGTAFVAELPAYNTVYSFRVTAVNEGGE
                     SFPSEELAVGINPAADGAVLIVNGFDRISGPAWFDRDGMAGVAWWDDRGVADRYSFIS
                     TGDQYDFERISPWTDDDNAGWGASYSNDEGRVIPGNTFDFTRVHGESVMAAGKSFFSV
                     SDEVFTGQDFNLSGWCAVDLLFGEEKTTTSAYWPDRKDFRIYTPEFLRALERMQKASL
                     PVFMSGSYPGTDLVMTNDTAVASLVKRTLHFMPRTGHAVRTGSVAATDKAAPVFSGRF
                     EFNAGISDKIYAAEAPDAIEPADKQSVTAFRYLENNTSAAVMYPGDARTFVMGFPFET
                     IISRKDRDDLMKQILDFLLK"
     gene            36131..37582
                     /locus_tag="PKK03_01330"
     CDS             36131..37582
                     /locus_tag="PKK03_01330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018966938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX83061.1"
                     /translation="MSVTATISSLRSPGLVSKVFVVLPPVSKGLPEGAEAGLITSEGF
                     AATDALRKMAATAADSDYVLTYSKGFPLDMGSHSLARMMQICDDTGAGMVYSDYYEKK
                     NDVMSPHPVIDYQEGALRDDFNFGSVILYSGKAFRDACDRMDQEYSFAGLYDLRLKIS
                     QKFPLIHIQELLYTEVETDTRKTGEKQFDYVDPRNRAVQVEMEKACTDHLRCIGGWLP
                     PRFRDVSFDDDCFGYDASVVIPVRNRVKTIGDAIRSVLDQKTRYRFNLIVVDNYSTDG
                     TTEIIRSFAGDSRLVHIIPGRKDLGIGGCWNEAVFSDHCGRFAIQLDSDDLYIDDSVV
                     TRVVDAFYEQQCAMVVGSYRMVNFDLDEIPPGLIDHREWTPDNGRNNALRINGLGAPR
                     AFYTPVLRKIRVPNVSYGEDYAVGLAISRHYRIGRIYEPLYLCRRWDENSDAVLDVAK
                     ANAHNLYKDRIRTIELLARKRMLAGSRKQDLND"
     gene            37575..38513
                     /locus_tag="PKK03_01335"
     CDS             37575..38513
                     /locus_tag="PKK03_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HNX83062.1"
                     /translation="MTDYSQKVRDLFEGQFREWTLARDNYSRLDGVMVRTVSFPGYDI
                     SVQFNPGRITSSAAKVDARSIEARSCFLCEKNRPREQRGVPFGDDYIILINPFPIFRR
                     HLTIVSRCHTPQRIAGSFYIMLSLAQTLPDYVIFYNGPQCGASAPDHLHFQAGNRGFL
                     PIEKDAVNPSLCRVVAEKNGTDLLLWSSYGRGILTLRGSDAEALSAAFARFHDRFALS
                     EPDRPEPMLNILAYHDEGRWTVHIIPRRVHRPRCYFAEGDSRILLSPASVDLGGVFIT
                     PRGEDFEKISAEDITNILAEVCLGEDELKTLTKEIL"
     gene            38510..39853
                     /locus_tag="PKK03_01340"
     CDS             38510..39853
                     /locus_tag="PKK03_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNX83063.1"
                     /translation="MTGKSEPEISVGIVTGSGLTFTLNGDFLAGDSGKMLSGMYTAVA
                     GADGLLLQSEAGSVRAGDEITFTPSDFGSCSFTLHAVTIGVNFHWQRNEDQTFRGNLK
                     FILRGGQVTAVNILPVEEYLVSVISSEMSATSSSELLKAHAVISRSWLLAQMDKTLQL
                     SDTGEKYRTSFITDDEITRWYDREDHRDFDVCADDHCQRYQGITRASTDAVEEAVRIT
                     GGEVLMYGDFICDARYSKCCGGATELFENVWEPVNHPYLRKVIDAGSGARTTDSDLTD
                     EKNATGWITGNPEAFCNTTDRHVLSQVLNNYDQETNDFFRWKVTYRQDELSELVRERT
                     GINFGRITSLEPLDRGTSGRITRLRITGDLKSMVIGKELEIRKALSKTHLYSSAFIVE
                     QSEENGEILFVLKGAGWGHGVGLCQIGAAVMGATGYTYRQILFHYFTGSEIKKLY"
     gene            39855..42326
                     /locus_tag="PKK03_01345"
     CDS             39855..42326
                     /locus_tag="PKK03_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HNX83064.1"
                     /translation="MKRVALTLVLAVLATGAAKADSWIRINQLGYLPGSVKVAVYLSQ
                     VPVAEKSFTVYDAFTDKPVFRGAAEICDGAVWGMAAAARLDFSGLTTPGGYYIIYSRT
                     KSETFRLGANVYDGTADFILNYIRQQRCGYNPFLADSCHRYDGMIVDHPTLSGTPIDV
                     RGGYHDASDHLRYTATTANSVYQMMFAYMMSPGIYGDNYDAAGVAGSNGIPDILDEVK
                     WGLEWLLKMNPDSGMMFNQVADDRDHRGFRLPNLDTTGYGLGPYRPVYFVTGRPQGLA
                     AFKNRTEGVASTAGKFASVFAMGARLFAQSDPLFARTMETRAADAFEFAHTDPGATQT
                     ACNVSPYFYEEDNYVDDMELAAWELWELTGNRYFLEQADYWGTLEPFTPWIEKDTARH
                     YQFYPFVNLGHASLGLSGTEYSVKYTEFMSKGLDIINSRTRDDVFQVRIPFVWCSNNY
                     VAAALTQCRLYREATGDDRFDYMEAAMRDWLFGCNPWGTSMICGLPAGGDYPLYPHSA
                     VTVFLGKTTTGGLVDGPISKAIHDNLRGLTLLRPDPYAAFQGGRTVYHDDIGDYSTNE
                     PTLDGTASLSYYLSTLEKEGRAERAASASDVYDGHGAIIRREAWEKSIHLVFSADEYG
                     EGLEHILNVLEDKNIRGSFFLTGNFLRNRRFDSTVKRMIAEGHYVGPHSDGHLLYIPW
                     ENRDTLLVTKQQFNDDLKRNIAALRKAGLKKEDPRWFLAPYEWYNSAISRWTEDLGMK
                     LVNFTPGTGTNADYTTPDMENYRSSDLLIQRLASFAESSPQGLNGAIILIHPGTAPER
                     TDKLWLRLAELIDYYTSKGYTFNRL"
     gene            42333..>43717
                     /locus_tag="PKK03_01350"
     CDS             42333..>43717
                     /locus_tag="PKK03_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX83065.1"
                     /translation="MAAQEKTRSPWAWIPTLYLAEGLPYVAVNIISVIMYKRMGISNA
                     DIALYTSLLYLPWVIKPLWSPLVDLLKTKRWWIVITQLVLALGFASIALTIPASFFFK
                     ITLAFFWLLAFGSATHDIAADGFYMLGLNTNQQAKYVGIRSTFYRIATITGQGLLVIL
                     AGFLESSSGREPVTLFIEASPQYSKTILQMPHVTENAAKPGDLYFIPDSDSLLVSTQT
                     VDRDSLGILLDSIKSSNLGNGFYKLEEKMAKEKGWWVSNISDPLGAWIRENFGEERGA
                     AASVTGSVAVVPVRISGKPAHGKEVVLNTALKKGDKSIALIQGEHLVFNETNWDKPAY
                     MVFQADSKLTGYTSVTYEGLSGNIPMAWAVTFFVLAGLFLVLSFYHRFVLPKPDSDRP
                     HENLKASDVLKEFGATFASFFRKPQVGAALFFMLTFRFAEAQLLKLINPFLLDPIDKG
                     GLGLTTGEVGFV"
     assembly_gap    43718..43817
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            43860..46868
                     /locus_tag="PKK03_01355"
     CDS             43860..46868
                     /locus_tag="PKK03_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNX83066.1"
                     /translation="MKKDFIFDPLRKELDGDLYTDDIQRILYATDASAYREIPVAVTR
                     PASVSDIRKILAFARGTGGSVIPRGAGTSLAGQVVGPGIVMDISRYLNHILEFNPAER
                     WVRVEPGVVLAELNRYLAPHRLQFGPETSTADRCCIGGMLGNNSCGAHSLIHGSVRDH
                     ILEVDAILSDGSEVHFGALTTDEFRKKCSGDPGLLETKIYRNLQEMLSDQRNAGEIRR
                     EYPHPALRRRNNGYALDALLETDPFTGNGQKFNVCKLLAGSEGTLAFTTSMKLNLVPV
                     TNDRTGLVCAHFGSLQDAVRANIIALKHNPAAVELIDDYILNCTKDNIEQNRNRFFVK
                     GDPKAILLIEITRPTVEEIERTAAAMEKDMVAAGFGYHFPLYTGADEMARVWELRKAG
                     LGVLLNIPGRRKSVQVIEDTAVLPDLFPDYIAEAAGILEKHRLSCAYYAHIATGELHL
                     SPLLDLKDPDDIRTFHGLAEDMAALVKKYRGSLSGEHGDGRLRGEFIPLMLGPHNYAL
                     LKELKKTWDPSGLFNPGKITDTPPITENLRYPAGERRQYPGLVFEFPGEEGLLYAAEK
                     CSGSADCRKSALVGGTMCPTFMATRDEDKSTRARANILREFLTRSDRKNKFDHEEIYR
                     VMDLCLSCKACKAECPSNVDVARLKAEFLQHYYDIHHVPFRAWLIAWLPRLYVPGMMF
                     RPLTNLVTGLPVFKRIIGFSVKRPVPALSPVTLRTWERRHRRKNGKLGASGSDRAAGL
                     SGTSGSQGSSRTENAGIPKGTVCLFADEFTNYNESDIGIKAVMLLERLGYKVIIPRHA
                     ESGRTFLSKGLLRSARKIAVKNIMLLRGVVSDERPLLGIEPSALLAFRDEYPEMAGRT
                     LADDARRLAGNSMLIEEFICREADRGNISAASFTDRPAEILLHGHCQQKAIASTAPTI
                     RMLSLPANYRVKEINDGCCGMAGAFGYEKEHYDLSMKIGEMVLFPAVREATAETVISA
                     PGTSCRHHIMDGTGRKALHPVEVLFDALIK"
     gene            46893..>48888
                     /locus_tag="PKK03_01360"
     CDS             46893..>48888
                     /locus_tag="PKK03_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83067.1"
                     /translation="MSTFGFSKVELAFFERSGRTKMTARMPYIVVDNFPGLGLLTSLR
                     FLEWVAGNPNGVISLPTGKTPEYFIKWTKYLLNNWDSPEARKIREDNGLFLKEKPSLR
                     SLHFVQIDEFYPIDPRQHNSFYDYVRNYYIEGFDLDPARALLINSEEIVLADGKHFSE
                     VFPDNIIDLSLRNREAVGPLEKLQQESLFRIDNWCTGYEKKIRALGGIGFFLGGIGPD
                     GHIAFNTRGSDHNSATRLTATNFETQAASAGDLGGIEVSRTRLVITIGLGTITFNPDA
                     VAIIFAAGDAKALVVRNALENEPDNLYPATVLQGLKSARFYLTAGAAVQLHDSVERYF
                     REGEWTFEKTERAVFDLCQKIEKYAHKLVPEDLKNDEYCSLIPGLSLNTVADVIESTK
                     RKIEAGLRRERNTTFLHTGPHHDDIMLGIFPAIIPQLREITNKFHFSVLTSGFTAVTN
                     TMLLGLLEETQRLISAGMIQMLRYPDFFESGYRFKWDKDVSHYLDKVADKDEAGKLRG
                     LCHRIVRAMVDIYRLKSKDDLNEQIRKNIELIRKSYSGEKNPPDIQRLKGMLREYEEE
                     LVWAHYGVPVRNIEHMRLGFYTGDIFTQQPDRHRDVEPILEYLRRIQPNIISLAFDPE
                     GSGPDTHYKVLQAIAEALRQWGLEKDLSNLRVIGYRNVWFR"
BASE COUNT        11378 a        12342 c        13156 g        11812 t
ORIGIN      
        1 agtacattgc catcccatct gttcttgcag gcctgatcgg ggtttggttt accgtcgaca
       61 aatggatgca gaatttcgcg gtaaaggttc cgatgcactg gggattgttt gtcctttgca
      121 gcctggcaat tctgttttca gtagcgcttg ttgcggcagt gaactattac aggtttgcca
      181 acagaaaccc ggttgaggcg ttaaggtacg agtagggggg gtgtggttat tcgcgagctt
      241 ccggtctgaa gtgattattc gctcgctgga gctcgctcat gatcactttt cgggggggtg
      301 actataagaa aaagcccggt actcgctgcg cgagcttccg gtctttaatt tctttctcgc
      361 tcctgaaagc aagctttcgt cgctcgaaaa aattaaagcc cgctccacct ttcggcgggc
      421 cgggcttttt cttatgtcgg ggtagcaaga ttcgaacttg cggccctctg gtcccaaacc
      481 agatgcgcta accggactgc gctatacccc gaatcggcga tgcaaaaata gaaagatttt
      541 tgtaacttcg gcctcccgga tagttttatt acgggcattg aaaccatttg gcaccaggtt
      601 tgttattact gaattaaatg actgaaaaaa tgaaaaaaaa actctttacc atggcagcac
      661 tgatggcctt catcctggca cccgccctgt ctcagaagtc gttctacgac tttaccgtcg
      721 agtccattga cggtcaacag tacagcatgg ctgacctgaa aggcaaaaag gtgatgatag
      781 tgaatactgc ttcaaaatgc ggccatactc cccagtacaa ggacctcgaa ctgctcaaca
      841 aggcatacaa acagaagctg gtcataatcg gattcccggc gaataatttc atgggccagg
      901 agcccggaag caacgaggaa atcaaacaat tctgcgacag caggtacgga gtgaccttcc
      961 cgatgatgtc aaaaatttcc gtcaagggcg atgacatgca ccccctctac agatggctca
     1021 ccagcaaaag cgcaaacggt gtgctcgact cggaagtatc atggaacttc cagaaatata
     1081 tcatagatga gaacggcaaa ctcgtgggct tcttcaaacc tgcagtcaaa cctctctctg
     1141 acgaggtggt taactgggtt acaaagggac agtttatcca ggcaaaataa attctgacat
     1201 acaaaaattg acggccggca cccccggccg tttttttact gccggcaccc ccggccgttt
     1261 ttttttgcgc cacagccagc gccatgcagt gtaaatccgc cgaccggatc cccagcagcc
     1321 gcaaacgttt taatcagcct cacgccgaac tttttgttta acctgttgtt atacataaaa
     1381 aataaacgtc atggcagatc tgagaacacg ctacatgggc atcgagctga agaaccctat
     1441 tattgctggt gccagcaaca tggtgactga catccacaag ctgcagaaac tggaacaggc
     1501 cggtgttgca gcaatcgtct acaaatctct cttcgaagag caggtacaac tcgagaatct
     1561 tgagctatac gaactcaaaa ctgagtatga gcacagacac gccgaacagg tgacgctgtt
     1621 ccccaactcc catacagtgc cggaatcacc tgaagactat ctccttcacc tgaagaaagc
     1681 ccgggaggct gtatctgttc ccttttttgc cagcctcaat gccattaatg acgaggtttg
     1741 ggttgaatac gcagtaaaag cagcagcaac aggtgttgac ggactggaac tgaatttcta
     1801 ttcgctccct gacgaatcgg gcaaggatcg tgaaacgatc gaaaagaaac aggtggagac
     1861 ccttcgcaag gtaaaagagg ccgtgaagat tccggtggct gtgaagctca ctccatacta
     1921 tacgaacacg cttaaattca tatctgagct tgacacagcc ggcactgacg cggttgttgt
     1981 tttcaacagg ctgattcagc ccgatatcga catatacagt gagaagcata caatgccgta
     2041 taacctcagc agcagcgagg acaacaagct cccgctcagg ttcaccggca tgctgcatgg
     2101 taatgtcagg tcgtccgttt gcacctcaac aggagtttac tcggcaaatg atgttataaa
     2161 gatgatcctg gccggggctg atgcggtgca ggttgtcagc acactctatc gcaacggggt
     2221 tgaagttgta gaatccatga ccggggagat tgaaaaatgg atggacggtc acggttacaa
     2281 ggaccttgac agcttccgcg ggaaactatc aatgaagaaa tcagctaaca agcttcccta
     2341 tacacgtgct cagtatatcg atttcatgct gaactcagga gacataatga agaagtaccg
     2401 ctcgctgagc tgaaacaacc ggtcccgggc attgcagccg gcggtacaaa tgtgccaccc
     2461 cgcggcacaa atgtgccacc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nacaaatgtg ccaccccgcg gcacaaatgt gccaccctgc ggcacaaatg tgccaccccg
     2641 cgacagacat aaaaagtttc tgataaatga ttcttccggc gctgtgacgg gtgccgtgct
     2701 caactgccag agatggccct gagcaacagc catgaagaaa ttgacgagag acggatggcc
     2761 tcactgagac cgtccgtttt tgtcaacagc ctgtcgttac cgcgaagcat cccgaggtag
     2821 agtccggcac aatcctcagg tgatatttcc cctgacacct cagtctccgg caaatgcctt
     2881 ctgaagagag gaataagtat ctgcatctca gccttctgaa gcttgtccct gagcttctct
     2941 atcctgctgt gcccctggga aaaatccttc atcaacaacg gattgcgcat gatcagatcg
     3001 cccagcagtt gcatcctctc ctcaccataa cgatgaatga ctgtcccgag ttccccgtca
     3061 gaccgtgcca gtgagctcag tggtccggtg atctgcctga tctccttctc aaccactgcg
     3121 tcatagatct gggctttgtt cctgaaatac atgtacacag tacgcctgcc acgtccggct
     3181 gcagccgcta tcgtctccat ggtggtcctt cgcaaaccca cggtggcaaa cagcccggca
     3241 gcgacgtcaa ttatcatatc acgtgtactc tttctagcca ttgcacaaaa taagaaatat
     3301 gtgcaaagtt agtaaagcga ggaaattata gtttaatttt aagggtttat tcattaaacg
     3361 gacagtaatg aaacggattt tgctagtttt tatgtgtctg cttccgttca cggcaggcgg
     3421 acaggttatt gttgacaagc ctctgagtgc taggcttaca gggtatgaca ttgacgttac
     3481 ccttgacccg gggaagcaca ctgtcagcgg ccagatgacg gcgtggtggg tcaatcactc
     3541 atccatgccg gtaggggagg cgatgatgca catgtatctc aacgccttca gcagcaacaa
     3601 gagtactttt gcctccggag gaagatggtc tgcatccggt gacgacggat ggggctgggt
     3661 caaaatcaaa tccatgaggg acggcagtgg caatgacctc atgggatcct gtgggtatgt
     3721 tgcacctgat gacggcaatg aaaatgacaa aacggtactg agggttgtcc ttcctgagcc
     3781 tgtggctccc ggagacactc ttgtactgaa gattgatttc atctccaagc tgccatcacc
     3841 gattcagcgg accggatatg atgatgattt tcacttcgtt gcacagtggt tcccgaagtt
     3901 tggggtctat gagacggctg gcatgcggca gcgtgaaact gacggttgga actgtcacca
     3961 gtttcatccc aactcagagt tctattcgaa ccattctgtt tacaacgtgt ccgtcaccct
     4021 tccctccgaa tttgttacag gctcaggggg aaaacttatt gaggagattg atctcggcgg
     4081 tggccttaag aaggaggtat ggagggctga ggatatagta gattttgcat ggaccgcatg
     4141 gccgggatac aaggtcgtga ccaggaaatg gaacaatgtt gacattacct tcctgaccag
     4201 taagatgggt ctgtcaaagg ctgaaaacca cctggatgca gttgtttatg cgcttgagta
     4261 tcttgccgcc agggtagggc cctatccctg gcctcacctg acatttgttg atcctcctct
     4321 ttccggctca ggtgcaggag ggatggagta caccactctg ttcaccacca tgggtggcgg
     4381 cgcagtgccg acatttatga agatgcccga gatggtgaca atacacgagt ttggtcatgc
     4441 atacttcatg ggaatcctcg caagcaatga gtttgaggag ccatggcttg atgaaggcgt
     4501 caattcgtac tgggagcagc gcattgttga ccattactat ggaggcggtt acggactcgt
     4561 caggcttccc ttcctgaaga tgtcagacac tgaccagggg aggctcagct acatcagctc
     4621 atcaaaccgt acaattgcct cgaatgatct cccttcgtgg atgtatcctc acgggaccta
     4681 ttccatgatg tcatacaaca aggcggcaac atggctgcac accctccagg gaattatcgg
     4741 tgatgagacc atggacaatg tcttcaggga atattaccgc cgctgggcct tccgccaccc
     4801 ttcggggcgc gactttattt cagtagtgaa cgatgttgtg aagaaagagc atggtgacag
     4861 gttcggtgag aacatgaact ggttctttga ccaggtgctt tacggcaagg gggaatgtga
     4921 ttacagagtc tcgggaatca caagcttcag gataaggagc tattctggcg tagtagatgg
     4981 cgactcggtg acatacgccc gcagcgaccg taaaagcgac acactttata tctcacgggt
     5041 aagtgtcgag aggttgggtg aagtcatgct gccggttgaa atccttgtca gatttgataa
     5101 cggagaggag gttctagaaa agtgggacgg gaaaacgagt tataaggatt atgagtatac
     5161 aggcgcccgt aaagtggtct gggccaagct cgatccgtat gaccggattg acatggatgt
     5221 gaaccggatc aacaactcat ggacgcttga gccaaaccgg acggccacca ggcgcatgct
     5281 gaacaagttt gttctcctga tgcagatgat gatttcaatt ttcacccttt aaattttccg
     5341 agatgagctt attcaagaca ctgaagaacg gcgctgctgc tgcggcatct tcatataaac
     5401 ttatactcct tatctgggtt acaaccctgg ttcttacgct ggcggtcggg tttccgctac
     5461 gttcgttcct gaatatgatg ttcgggagca gcctggcaat tgagcgtctc aatgacggat
     5521 ttgacctcgg cattgccgga gacctcggca aatccctggg cgggctgatg gcgtcagttt
     5581 ctgccggcac aatgcttatc agccttgccg gtttctttat catgacattc tttgcaggtg
     5641 gtcttttcag gcgtttctcc ctggcatggg gagggctgaa ggtctctgat ttcctgagag
     5701 catcagcagg caacttcatc ccgtttctca agatcgcgct gctgatgatt ctgattatag
     5761 gagcctacac cttcgtgctg attggtattc ccgtgattgc cggcctgatt gcctccggtt
     5821 cgccccgtca gaacgggaag tttttttatc tcttctatgt aatatgggct cttggtctgc
     5881 cggtatggct ttttgtagcc gatgcctcaa ggagatggat agccgccacg ggatcgcgga
     5941 aggtgttcag ggcactcggg gcaggcttca gggcaatcag ggagaggttc tggcactcat
     6001 acctgacagt ccttgccgtc attatcttca atgtaatatt tatcgcggcg ctgatgtggt
     6061 ttgccgccac ttcaactccc gaaaagggaa taatggtttt cctctttttt atcgccaccc
     6121 aggcgctctt catcataagg ctcttcatga aggcctggcg gtacgcctca gtgtgcgaag
     6181 ccgtgcagca ggtgaaaagc tgacggtgtc ctgcaaaaaa cagataaata ataaatatca
     6241 acctaacctg acagtaatga aaagatgcac ctccggaatt gttccggcaa tcttgttggt
     6301 tatccttttg tttgttatgc ctttccggct ctctgcccag gcaaaacagc cggtcctcaa
     6361 gggaaccggg cgtgtgaaga tgttcgccgc ccaggccggt ctgagggctt catcaccata
     6421 taaggacctg cactggcagt acataggtcc gaccaatatc agcggacggt gcactgacgt
     6481 cgaagcagtt tcgcccaggg gcggacaata cacaatctgg gtgggatcag caaccggcgg
     6541 tgtgtggaaa tcagtcaatg agggaaccac ttttgaatcc gtatttgacg agatgcctac
     6601 agcctcaatt ggtgacattg caattgaccc gaagaacccg gatgtcgtat gggttggtac
     6661 cggtgaggcc aatatcttcc gaagctccaa tgcaggatgc ggtgtattta aaaccactga
     6721 cggaggcaag acctgggacc ttatgggcct tgagaacacc cacactatcg ggagaatacg
     6781 ggtcaatccc gggaacactg acattgttta tgttgcctct cccggccatg agtggacccc
     6841 gaacgaggag cgcggattgt ataagaccgc cgatggcggg aagaactgga cgaagatcct
     6901 ctcaataaat cagaataccg gtgtctgtga catagtgctt gatcctcgtg atccgaacac
     6961 gctgtacgct accacctggg aacgtacgag gcttaagtgg aatgatcccc gcacatttgc
     7021 ggatacacgc aattgcggcg tctggaaatc gactgacggc ggcaaaacct ggaaggagat
     7081 caacgcgggg cttcccgcac cgcagcaccg cggtcgcata ggcgtggata tatctctctc
     7141 caatcctgat gtgctttacc tgttgcttga caactacgag atagcctata aggcaaagcc
     7201 cggtgaactt gattcttacg gccgtcagaa gcaggatgtc atcaaaggag ccacggttta
     7261 ccgttcgggt gacgccgggg cgtcatggaa gcaggtcagc gggctcaccg gggaaaccag
     7321 ggtttatatg gagtatcact cgggaactta tggatgggtc ttcgggcaga tcagggttga
     7381 cccgaaggat gagaacacca tatttacgct tggcattgag ctccacaaat cggttgacgg
     7441 aggtgcaacc ttcaggaccc tcgacgggcc ccatgcagac catcacggat tatggataga
     7501 cccgaacaat cccaattacc tgcttaatgt acaggatggc ggcctgacaa tatcgtatga
     7561 caagggtgcc acctggaaat atcctattgc ggagcttcct cttgcacagt tctacaacat
     7621 cgcgtatgac ttcagcgtac ctttcagggt attcggctcg attcaggatc atcacagctt
     7681 ctacgggggt gtagatcttt cgcgcgggcg cgacagggta cgtcccgtgg aattcaggaa
     7741 caccctaggg gcagagggga gttctcatgc ggttgacccg cgcgacaata acacaattta
     7801 ttcaagtacc ttctacggtg cgctggccag ggcagagata gacaattacc cggcaagccg
     7861 caaggacctc cttccggtaa ggtatgccga cgagccgagg ttacgcgggc agtgggttgc
     7921 tcctacgctt ctctcaccgc ataatcccga cattgtttac catgggatgc agtatgtgat
     7981 gatgtcacgc gataagggta acacatggga gttcataagc cctgacctca ccttcaatga
     8041 ccccaaaaag cagggcgaca taagctatca gaccatccag acattggatg aatctcccat
     8101 gagggcagga cttctctatg caggcactga tgacggcagg atctggcgta cgaaagacgg
     8161 cggaaagatc tggaccgagc tcagaccgaa gaatgttccg gtaaggtggg tgtctcgtgt
     8221 ggtggcatca aaatatgatc ttggcacagt gtatatgacc cagaccggca ggcgtgatga
     8281 tgatttccag gtatatgtct ggaaatcaac cgattttggt gatacatgga ctgatatctc
     8341 aggaaatatt cctgtgggac cggtgaatgt catcagggag gatccggtca ggaaggatct
     8401 gctctatgca ggaactgatg ccggtgttta tgtcagcaag gacggtggcg cccgctggga
     8461 ggtgctgggt gatcttcctt tcgcgtatgt gcatgacctg cagattcacc ctcgtgacaa
     8521 tatgataatc attgccacgc acggacgtgg catgttcgtt cttgatgcca atcctgtcaa
     8581 cgagaaagac aagatgagaa aacggtggta ttaaacagct gcctgttgcc ggatttgtct
     8641 gttactgcgg tgtgtgcaac cggattttgg atttccggaa tgatagtgct ttctttatgc
     8701 ccctgaaaga aaaacaggca ggatggcatt gacaacaggt acaaacggaa acggggaaag
     8761 ggtgaaactg atcaggaaaa ttcaccgcct gacagcctca tttctctctg tggccctgat
     8821 catcgtagct gtctcgggga tactcctcgg ctggaaaaag aacagcaacg ggtatctgca
     8881 tccggtctcc catgagggga ctacttctga tttccggcaa tggcttccgc ttgactcgct
     8941 taacgcttta gctgtcaggg cacttcatgc cacaggaaga aaagggctca gcgaggagct
     9001 agacaggatt gacataaggc ctgacaaagg gatggtgaag ttcgtgttcg cacatcacta
     9061 ccatggcatt cagcttgacg gcgcaacagg agaggtgctc tatgtggaga acaggagatc
     9121 agacctggtt gaagacatac atgacatgtc agtgatagac agattgtttg gcattgatgg
     9181 agagttgttc aagcttatct ataccacctt taccggcctg tcattgctgg tgttcacggc
     9241 taccggcata tggctccgat acttttcccg ccgggggaaa gagaaaaaga gatagtctga
     9301 cctcccgggc aagacgacat gctcgaccgc ggccggcgta ctcatttaaa attcaggcag
     9361 cctgtagaac catgcggtga ccgaaaagtc gattgttgtt ttcacccagt gccacatttc
     9421 gatgtcggcc cggagtgatt ccctgaatgg tatcgcatcc agtatcctgt agcgcatatt
     9481 cacgctcatc cccgggtgaa agttcccata tcctgccggt tgtgcaatca gagggtggtc
     9541 aaacagttcc ggacggcacc atgcatatcc gtagtaatcc tccgttcctg ttccgaagga
     9601 tgatgggaaa gtttcgccgt cggtgtagat cttctcatca ccctcgcccc accaggcgtc
     9661 agcagtgttg aaaaccagca ctgcatcacc ggcatataac cctttccccc tgagcccggc
     9721 tatattcacg tcagtgtgca gtcccgttcc cccggtgttt tctgagccgg ctgtttcaaa
     9781 ccccgggtac tccttccatg acgctccgaa atgcattgag ttttcattcc acctgtaggg
     9841 tgccagggta accagcgttt ttacagcaat tgtgtcatcg gtactgttga ggatgcttat
     9901 tctgcagctg tcacggaatg gcatcagcca tgaagagctc agcgtgccgt cggtctcagc
     9961 ggccgtgaag cgggtgtgac aggagttgta tgtgtagccg gtgccgaaga agttgccagc
    10021 cggcacctcc acggtactgt ggccatcgaa ccacatccgg atgatggtcc ctttcagcag
    10081 ggctgcagta tcggatccgg agagactgag tgagaggttg ctgacagcaa ggtttttccc
    10141 cgtaactgat atccggcatt ccgtgccggg agggaacatg ccatcggatg atctctgctc
    10201 tttgcccgtt gtctggttgc tgcccgccct gtcgaggtcc gcagcacatt ctgcgatggc
    10261 aagggtgtca tcacgaaacg tttcgctttt tacagaggca accctgatgc cctcttcata
    10321 tagccggaca tcaatgttgt aataaatcga gggtttgcgt ctctgtggtg tgatgagaac
    10381 ggagtcgttt tcaagggtga tgatgcactt ttcagcaaac ggtatgggaa ggtagaggtt
    10441 gtaaccgcgg cgctgcggtt ccgtcagtgg tgagactgat gtgctcaggg gtgcgggggc
    10501 caggacaccc ccgccaacca gttccggagc catgccttca attaccggtt cggtccggcc
    10561 gtcgagataa accctgatgt atgagttcag ggcgtcttca tctccgaatg tcatccacca
    10621 gcggacgatg gcgccggggc cgtctgcttc gagcattaca tattcacacc ggccgccggt
    10681 agtctcctcg cggatgaaat gggtatagtc attgttggcg taccatcctg cgctgtcagg
    10741 gtggatgctg cgtctgtcgt atgagctgaa ttgcaccagc ctgtagtctg gtgtgggata
    10801 ccgtgtcagc gtctctgcgg aggtcatctc ccggagcagt gaccggaagg tgatcacggt
    10861 ttcatttttg caggaagaga gcagacaaac ggcggccata agcaggaaca ggtggctgat
    10921 gattccggtt tttttcattg ggcaggggtt tgggaataaa gttattcatt cttttacaat
    10981 gctgtatgcc ctgcgggcaa ctcagcggtt tcatatagcc aaatgaatgc aattctgtat
    11041 gccatgcggg caatagtggt aatccatggc actattgcct attgccttac tttgctgccg
    11101 attgcctact ggtactgcct tcccgatccg ccagccggcg gatacgggat tgagccacct
    11161 cctacgtcgg agtctcaaac tgcctactga tactgcccac tgcctgcttc taagtataaa
    11221 tactcccgcc aaaatgggtg ttaatcccgt tcccggcgtc ataagtcatt gctaattttg
    11281 aacttcccta aaatggcaat atccgaactt atggaagatc tctattcaag gctgttgcag
    11341 gacatacgtt tcgaagaggg actgaaggcc tgcatgaact gcgggatatg cacaggcata
    11401 tgtccggcag ccgaatatta caactatgat ccgcgccggg ttgtcgacct cgttcagacc
    11461 cgcaacaatg atgttatcag ggagttgctg aagagcgaga ccatatggta ctgcgggcag
    11521 tgcatgagct gcaagacccg gtgccccagg ggcaacaccc ctggcatggt catcatggcc
    11581 ctccgtaagc tgtcacagga actcggatac ttcactgcat ccgagaaggg gagacagcag
    11641 ttcgccatca agcgtacagt gggcgacaat gttctcaagc atggctactg tgttgtctca
    11701 tatgacgtgg accctgaggc acatccggaa cagggcccgg catgggaaca ctattacaat
    11761 cacatggatg actcgcttgc ccgtctcggc ggtgagctca acggcaggga gggtcccaca
    11821 aggcttatcc ctgatgatgt gctggatgag ctgaagagca tttttaaagt gaccggcgga
    11881 caggatctat atgatgccat cgaagaggcc tccgagaaga aggctgaaga gatgggaatg
    11941 gagttcaaaa aggaagggcg tgattttgaa tacttcaggc atgtggtctc ggccgacagc
    12001 ggctgccacc agaatgaacc tgaagacata aggaaggatg tatatgaaac cggaaggtaa
    12061 gaagaggatc tgggcagaat accagaagga gatccctgac gatcatttct tttatgtcag
    12121 gtcatgcatc aggcagacat tctttgccgg ctcggagcag tatttcctga aaattctccg
    12181 tgatgatctg ggcaaggatg tcttcgagga tccccggcac acaacctgca ctggcattgg
    12241 ctaccacggt gatgttgtgc cgtttgaaac gatacaggct gtagtggcac gccattttgc
    12301 gctcgccacc gaagccgggt acaggcacat agcaatctca tgtgtcacct cttttggcat
    12361 ttattcagag atactcgaga catggaaaca ttttccggag acgcttgaac gaacccgtga
    12421 ggatctgtat aaggccacag ggcgcacatt tgaagttccg gaaaccatat cgcacaccag
    12481 tgacatcatc tataagttca gggatcagat agcccgcaag gcgaagtaca atcttgtcga
    12541 cataaacacc ggcaaaccat tgaaggtggt tgaacacatc ggatgccatt actcgaagat
    12601 gttcccgaag tacggggtgg gcggggctga gttcccgcat gtgctggttg gcatggttga
    12661 agcatggggc ggtgatatca ttgattaccc ggaaaggcgt cactgttgcg gcttcggctt
    12721 cagacagtac ctggtgcagg ccaacagggg ctactcgctc tttgcctcgc gaaagaagtt
    12781 tgagtcgatg gaaccttaca ggcccgatat gataattgcc aactgccctg gctgcaccat
    12841 gttcatggac cgctggcagt acaccatcag ggagattgac ggcattacct acggtgctga
    12901 tggccaggga attcccgtcc tgacttatga ggaggtcgcc ggcctgattc tgggatacaa
    12961 tccatgggag ctcgggcttc agtaccataa tgtgcagtcg cttcccctgc ttcgcaggat
    13021 gggtatcaag gcagatcctt cggagcgctt cctcgcgaag gatggctcca ggctgcccga
    13081 gcctgccaac ctgataaacg gatgaagacc acctcagcgc acatagtagt tgccggaggc
    13141 ggtgttgccg gcatggaagc ggcaggcacc cttgccgaaa tgggctatcg tgtcaccatg
    13201 atcgaaaagg atgacgagcc cggagggcac ctgttgcagt ggcatgctct cttccccgac
    13261 cgccgcccgg cggatgaggt gagaaactat ctcagaaaac atattgagca acgcaacctg
    13321 agcatcatca ccgggaccac ggtagaaaag atcaggcacc atggcagcgg cgtgctcgtg
    13381 agcctctccg gagggactga agagaaggct gatgcggtga tagtggccac cgggtttgac
    13441 ctcttcgatg cccgcaggaa agaggagtac ggttacggga tctacgacaa tgtcattaca
    13501 tccgcagagc tcgaggggat gttcaacagg ggccgcgtgg caaaggcaga cggaagcgtg
    13561 cctgcacgta tcgggcttat tcactgtgtg ggctcacgtg atgagaaggt gaacaacaac
    13621 cactgctcca aggtatgctg cgttaccgcc gtaaaacagg cgatagagat caggaagatg
    13681 cttccctcgt gcgaggtctt ctgtttttac atggacatgc gtatgtacgg tccttactac
    13741 gaggaacttt accgcgagtc acaggagttt cacaacgtta atttcatcag gggcaaggtg
    13801 tcagaagcat ctgtcaatat aaacaacagg ctggtgatca aggtggagga cacccttgca
    13861 ggcagaccgt tgaagatgga acttgacatt atggtactga tggtcgggat ggagatggcc
    13921 ggggccggga tgaagctggc agaatcctcc gggatgaaga gagggatcaa ccggttcctc
    13981 gagaccgctg atcaccacta tgggaacaat ctcagcggga taaagggcat cttttacgca
    14041 ggcacctgca cagctccgat gaatatcacc gataccattt cgcacgcgag ggcggcagtg
    14101 gcggaaacag taagatacct taacagcaga tgagcatcgc gcagtcaaat aagtttggtt
    14161 tcaccatcaa ccggagccgg ctgattgact atgactcctc tgacaggaaa gtacgggaat
    14221 ttatcactct gaaggagcca agtttcaggc tgtgttttgc ctgtggcggg tgtactgcaa
    14281 catgcactgc tggccagcat acctcattta acctgaggcg gatgaacacc tacatcagga
    14341 ggggagagat tgaacctctc cgtgaagagg tgaagcgctg tatgctctgc ggcaaatgcc
    14401 tgcttgtctg tccgcggggg gtaaatacac gcaatgtggt ggcagcggct gccgatgcaa
    14461 taggaaaact ggagcggaat gaactctgag ctgtttacat taccgttcaa cctggggctg
    14521 tacttcatat tgatatacag tgtggtgcga agcaccgtat ggttccggaa cctttcccgc
    14581 tcagacaaac tgcgtctgca gaggggcttc ttcgggaggc cgttcatgga cagtctgaga
    14641 gagatatcca tggagagcct tttgcatctc aagatcttca gaaaaaaccc ggtgctgggg
    14701 tatatgcaca tgagccttgc cttcggctgg ttcctcctga tagtatttgg caccatcgag
    14761 gccgacttct tcggggagaa gcatctcaac ccgccgtcgc acgccatttt cttccgctgg
    14821 tttaatcctg accacggggc tgaagggttt gcaggcatat acagtttctg gatggacctc
    14881 ctgctggcat tcatcctgtc aggactgctg ctggcgatta tcaagagatt cactccccgg
    14941 ctgatgggca tgaagaagac aacgaagcac acctggacag accgcatcgc ccttaccagc
    15001 ctctggctga ttttcccctc gaggctgctg gcagaaagtt tcacctgcgg ggtgcacggt
    15061 agcggcagct tcctgaccgg aaacctgggg aatgcccttg catcgttcct gccggcggca
    15121 gatatagcgc cctggttctg gtggctctat tccctctctc tcggaacatt ctttttcctt
    15181 cttcctgtat caaggtattt tcacataccg gtggagcttt tcctgatctt catgcgcaac
    15241 tccggcatct ccacaggcga caggcattca tcttacaccg aggtgcagac gcatgcctgt
    15301 tcatcatgcg gcgtctgcat cagcacatgc cagatgagct atgctgcggg gatacatgac
    15361 atacagtcgg cgtactttat caagggcctg aggaacgacc gtgacatcgc aggcataaca
    15421 gccaactgcc taatgtgtgg aagatgcgag gaggtatgcc ctgtgggcat cgaacttggt
    15481 cccatgaggc tgattgagcg gagacagggt gagaggagtg acaggaccct gaagctgcag
    15541 gagaaatatt cgctgatgcg aagaagaagc actcttgaga ggagaccgac acagtcatac
    15601 agataccttg tcagggaaga gcctgagaag gccgacgtgc tcttttttgc cggatgcatg
    15661 acccacctca ccccctcagt gattgtcgcc atgaagaaga tcatggatag cgccggggtc
    15721 aggtaccggt tcatggatga aaacggagga gtctgctgcg gaaggccgct gatgcttgcc
    15781 ggaagggaca gggaggcacg ggaactgata aaccataact cagacgttat ctggaaatcg
    15841 ggtgcacaga tactggtcac gtcatgtccc atctgctaca aggtcttcag ggagagctac
    15901 tatcttgaag cccgggtaat gcaccactct gagtttatca acctgcttat agaagaaggc
    15961 tcgctccgtc tcggttacct tcacaaaaag gtggtatacc atgacccgtg cgagcttgga
    16021 cgcggttcag gcatttacga agagcccagg gcggtcataa gctatgtggc agagctgcag
    16081 tcatcggagc aggagaggaa catgtcactc tgctgtggcg gcagcctggg caatctcacc
    16141 ctgtcatccg gaaaacgggc gatgatagct gccgatgcgg cccgcacgct cacccttggt
    16201 ttgcctgatg aactgattac agcctgtccg ctttgcaaga aaacctttgt ccaggccact
    16261 gaaaccaaag ttagcgatat cgccgaactg gtggcggctg ccctcccggc acccagaagg
    16321 gagaacgcaa aaaagagacc tgcaagaaga cactcccggg caaccagcaa tggttgaaaa
    16381 aaaatttagg aggttgcaaa tatttgaatt attgacgctt cgtattccaa ttatttccat
    16441 ttactttggc gccataattt ttcgggataa ctctggaata gtttttgccg gtaataaaaa
    16501 aaacattagt cgaagaatga aaagaatatt ttttgctgca gttgttttgg gtgttgcggt
    16561 attttccggt cttacgtcat gcaagaatga cgccggcctc aacggccagg agggtaagaa
    16621 caggaaaggc gccaccgttg aaggggaggc tgttgttctg agtatcggtg aggcagaact
    16681 tatagaggtt gatgacaacc cgcagtacaa tactgccgaa tggcttttca aggttgagaa
    16741 acccggccgc tgggatgtat ggctttcaag cctgacagtt gacaccaccc agcttcattt
    16801 cgcagagaat gtaattatta cagcaggtga gaccaagctt gaaaagaagc cggtcagtga
    16861 ccggatagtc cttggagaca agagcattac cgaaccctgg tacagggctg actcacatat
    16921 gggatccgta tttttctcaa aacccggtga ataccaggtt caggttatca gtgacaaggt
    16981 agagccacat tcttctgatc tttcacagct cagtattgcg aagcataccc tgatcaattc
    17041 agtgatactg aagcccaagg tcaattaacc gttgctctgg cgcccggggc gctgccaact
    17101 ttactttttc tttccctcga cccatttgcg ggcatttacg aatgcctgca tccacggtgt
    17161 aatgtcttca ttgcgcctgt cagcggggta gtatgcacac tgccaaggca tgaatgacct
    17221 ctcgagatgg ggcatcattg ccaggtgcct gccgtcggca gagcagattc ctgccacatc
    17281 gtacattgac ccattgggat tggccggata gtggctccgg ctgtatttca tcacgacgtt
    17341 ataatttttt tcaggcagcg ggagggagaa gcggccttca ccatgggcca cccatattcc
    17401 cagctccatt ccttcgaggc cggtgaacat aacagatttg ccgtcagtaa tcttcactcc
    17461 caggaagtct gattcgaact tatgagaggc attgtgttcc agcctgggca tctctttgtg
    17521 ttccggcaca atcagcccga gttctgccat cagctggcat ccgttgcaga cgcccagtga
    17581 gagggtgtct tctctcatgt agaaatcttc cagtgctatc cgggccttct cgttgtaccg
    17641 gaatgctccc gcccatcctt tggctgaacc gagaacgtcg gaatttgaga agccgccgac
    17701 gaagacaatc atgctgaggt cctgcagtgt ctcacgtcct gagatgaggt ctgtcatatg
    17761 cacgtccctg acgtcaaatc ccgccagcca gagggcataa gccatctcac ggtcaccgtt
    17821 cacccctttc tccctgatga ttgcggccct gactcccgtg gttgccttcc ggtcaggtgt
    17881 gatgccctgt gaccggaagc tgcctttaaa gttcttcagg tcgaacttaa tcgccttttc
    17941 gccatggttg tcataccgct ccctggcaag tacaggtccg cactgcctgc ggtcaagaag
    18001 gtatgagctc ctgtaccata gcgagcggca acggtcgatg tcaaactcaa aatggttatc
    18061 gccgttgact attgacagtt ttctttctct tacaggatgt ccgatggatg cgaaacgtac
    18121 acctctttca tagaggaatt cctctgtctc agcctcatcc ctgacctgga tgatcacacc
    18181 ggggttctgg ctgtagagca gcctgacggt atcggtctct ccgatctccg taaggttcag
    18241 ggtcattcct ccctcgcggt ttgcaaaaca catctccagg agggtgacaa tcattcctcc
    18301 tgcagagatg tcgtgacctg caatgatcct gtcgtcttta atcatcagtt gcagggtctc
    18361 gaatgtggct gcgaagtact ccgcatctgc cactccgggc gccacggttc ccaggctgcc
    18421 gatggtctgg ctgaacgccg aacctccagg ctcgtgcctc atgccgctca tgtcaatcca
    18481 gaagagtgaa gcggactcat catttcttat caccgggccg gtaatgcgcc ttatatcttc
    18541 taccccggct gcagcggtga tgatgacggt gccgggactg tataccgttc cgtcagggta
    18601 tttctgggtc attgagaggc tgtccttgcc ggtggggata tttattccca gcgctaccgc
    18661 gaagttgctg gccgcctgta ccgcttcata gagtcttgca tcctctcccg ggtttttaca
    18721 cggccacatc cagttggccg acagggagac gctcttcagt ccgtcaggca ttggagccca
    18781 gacgatattt gtgagagctt cggctatcga gaggactgat ccggcggcag cgctgacgag
    18841 gccggctgca ggagcatgac cgagtgaggt ggccatgccg ttcatgcccc ggtaatcgag
    18901 ggctatggcc ccgaggtcat ttagaggaag ttgcagttca ccggcacact gctgtacagc
    18961 tatccttccg gtcacggaac ggtcaacctt gtttgtcagc cagtccttgc atgccacctc
    19021 ctcaagctgt aacactgcat cgagatatct tgccgggtca gaaggctcat agatgagatc
    19081 tccggtcagg ctcttcatgg tggtgtcatt catgaccgtc cgcggaggtt tcccgaagaa
    19141 gtctgctagt tgcaggtcga tgggtctgtt gccgccggac ggattaagga aggtgaactg
    19201 gtggtcacct gttacctcgc cgatgacgta tatgggagcc cgttcgcggc gggccaccga
    19261 agtcagctgg tccacatcgc tgcttcttat cacaaggccc atccgttcct gtgattcgtt
    19321 gcctatgatc tccctggctg agagggtggg gtcacccacc ggcagtttgc tgatgtcaat
    19381 ggtgccgccg gttgattcga ccagttcgga aagacaattg aggtggcctc cggcgccgtg
    19441 gtcatggagt gatatcaccg ggttatggtc actttcggcc atggccctga gggcattgta
    19501 taccctcttc tgcatctcgg gattggcgcg ctgtacggcg ttgagctcga tggcgctgtc
    19561 ataggctccg gtatccaccg aggagacagc ccctccgccc atcccgatcc tgtaattgtc
    19621 gccgccgagg agcacaatca ggtcaccttt ttcgggtgta ttcttggtgc tgtcggattt
    19681 cttcccgaga ccgataccgc ctgcaagcat tatcaccttg tcaaaaccat agcgcttcat
    19741 gtcagtgaaa tgttcgaagg tcagcaccga gccgcagata aggggctggc cgaacttgtt
    19801 accgaagtcg ctggcgccat ttgaagcttt gatcagtatc tcttcaggtg tctggtagag
    19861 ccatttgcgc ggctcactgt acttttccca ttcgcgtggt ccgtcaggcc tggggtagga
    19921 ggtcatatag acggctgtgc cggcaatggg cagggagccc ttgccgcccg cgatacggtc
    19981 acgtatctcg ccgccggtgc cggtggaggc tccgttgaat ggctcaacgg tagtggggaa
    20041 gttatgggtt tcagccttca gggtaagcac cgactggata tattttgcac agaaataatc
    20101 aggtttgtcc tgggttgccg gggcgaactg ttcggtgaca ggcccgtcaa ggaaggcaca
    20161 gttgtctttg tatgccgaaa cgacgtggtt acggttttct ttcgtggtac gtttgatcag
    20221 gtcgaagagt gatgatttgc gttcttctcc gtcaatgatg aatgtgccgt tgaagatctt
    20281 gtgtctgcag tgctcggagt tgacctgtga gaatccgaat acttcgctgt ctgtaagttt
    20341 tcttcccagg ttacggctaa ggttttcaag gtatctgatc tcctcagcgc tcagcgcaag
    20401 tccttcctgt gcattgtatg cagctatgtc atcaatctcc cgtaccggtt ccggctcatg
    20461 gtgcacggta aagatatcct ggtcaagtcc gtgataaatc ctctgaagca tgtgatcatg
    20521 agccgggttt tcaccagcgg ctggaaagta ctcctcgatc cttgagatgc ctgacactgc
    20581 catgttctgt gttatctcca cggcgttcgt gctccagggg gttatcatct cgcgtctggg
    20641 tccgatgaat gtgccactga cggtctccgc ttccatgagg ctggcgttgc cgaaaagcca
    20701 tgagagcttt gccgtgtcat cccgggtgag ttgtccttct gccctgacag cgataacatt
    20761 cccggaggaa gttctgaaga aaagtacggt accagaggtc atagtatgct gatgaatagc
    20821 gaagtaacgg ctgcggtgcc gctactgcag tcgcttccaa agataataaa acaactgttg
    20881 aacctgaaaa ggaatactta gccggataac tattccttta actctccggt ctgaattcct
    20941 gcctgaaatc agtgcagcat aagggtatcc gggtcgcctg atatctcgat ggatccgttt
    21001 ctgccctcac cgctgaagat aacacggccg gttgatgtct cagaaagcat aaggcttatt
    21061 gccgaagtca tgctttcctc tatgcggcct cccatgaatc ctttaatcgg tgctgccagc
    21121 ggtgtcgccg tatccctggg agctgagact gagagggtat gtttcctgtt cctgaatgtg
    21181 atttccactt tctgtcgggt tacctgaagg ctgacgatct ttgatcctcc gtactctgtg
    21241 aaggggtaga gacggccatc gatcatcagt ccgctgataa atcctgtgaa atttcctgtc
    21301 agccatggta tgcgggcaac ggaggccttg aaggagatgc cattggctgc gaagtgattc
    21361 gactgcatcc agatatacgc cgaggggaac gagtggcccc agtctttttc gatatagccc
    21421 ctgccaccgg tgaaatcaat ttcctccccg tttatgacga ggctgccact gatgccgtgg
    21481 tccatgctca cgataccgtg attgcactcc atgaacggtg caaatgtgta cggccccata
    21541 attccggggg cgtaccatgg atttggccat tctctggtac cagtgaaagt aagttctccg
    21601 ctgactgaag gcagttccag tctcatccgg ctcgcagaaa agaaattgtt gccgacagtc
    21661 accatgaagt ttttttcgga tggaacaaat gcggtggatt caaacgggaa atactctgct
    21721 gtttttttct tgccgtcgag gacctgtaca aacgcctgtt tgtttccggc ttcattcatt
    21781 gaaattcccg gaataaacgc aaaggctgcg gtctctggcg cattgaccac cttgaaatac
    21841 cacccctcga aatacctgcg ggtattgccc cagccctggt acatggaagg attaaagagg
    21901 gatctgattt ttgtaagcat aatcacgagg ttcagggtag aggttcagga ctaaagataa
    21961 gcaatttgag aatcgccgct gaaaaacctg aaatccttcc cgtgattgtc ttcaatcgcc
    22021 acggccgagg cccgatgcat aggttgccag ggcgcgctgg cgtgccatcg catggtcaat
    22081 catgggtgaa ggataggagg ggctgccgag ttcgggcacc caccggttta tgtagcgcat
    22141 ctgcggatca aacttacgtg tctgttctgc ggggttgaac accctgaagt agggggcggc
    22201 atcgcagccg gtgccggctg cccactgcca gttgccgtta tttgatgaga gctcgtagtc
    22261 aagtagcttt ccggcgaagt atgcctcgcc ccatcgccag tctgtaagca ggtgctttgt
    22321 caggaagctg gcagtgatca tacggactct gttgtgcatg aagccggtgg tattaagctc
    22381 acgcatgcct gcatccacta tcgggtaacc ggtcatgccg ttgcaccacc tgtcaaactc
    22441 ctcttcgttg ttgcgccact gtattgcgtc atacctggct ttgaatgaac tgtcggttac
    22501 atgcgggaag tgccagatga tgctcatgaa aaattctctc cagatgagct cattcagcca
    22561 ggtttcattg agatcgagtg cggtcctgac cagctgccgg atgctgacgg ttccgaaacg
    22621 caggtgaaca ccaagcctcg ttgtcccctc aagtgaggga atgtcgcggg taaggtgata
    22681 attcctgata atttcctggt ttattgccgg taccggcgaa acagtgtcag tgtttgtgaa
    22741 actgatttct tccggaccgg ggattctggc agccggcaac ttcaagaaat tgccggcatg
    22801 cttttccgag aagaatgatt ccgtgtcctg aatagtgagc tgtttcttcc aggcccgtga
    22861 atatggcgta aaaaccgtat aaggcgtacc gtcagacttg accacctctt tcttctcaag
    22921 gatcacctgg tccttgaatg agtggaacgg aatcgcggag tttttcagga ggagtgaaat
    22981 cttttcatcg cgggcagtgg cgtatggctc ataatcgcgg ttggtgaaaa ccgcctttac
    23041 atcatactca tccaggatct tccggaaggc ttccacgggt gttctgtgaa gaaccttcag
    23101 cgatgagccg gcagacgcaa gctttttatt tatttccccc agcgccgcca ggatgaatgc
    23161 caccctccgg tctgccttct cttcgagcct gtcaaggatt tcggtgtcga gaatgaaaac
    23221 cggcaacacc gggtgccctg acctcagggc atggtaaaga cccacattgt cttccaggcg
    23281 caggtcgcgc ctgaaccaga agactgcaat ggcatccttc tgtcgcataa tctcttcagg
    23341 ctcttaagtt ttcattgtgc gaccggcgca gcctctttct tactgaggta aagatcagga
    23401 ttgtgagcag cagaaagaga gccaggataa aaaacggtat tacaagtgaa aggatggaag
    23461 tgcctgtggc ggccacgttc tctactgttg ctaccaccgg gttgcccagg ccggccgtca
    23521 gcttagtcga ggtgagcctt gtcagggcgc tgccgctctg caccacggca gcgctgccac
    23581 cgcccacggc agcgccggtg atccacttca tgagctcgct gtctatcggc agcaccgagg
    23641 ttagcaggag ggtcccggca cctatcgcca ggggcgtgga gacagtatca agcaggttgt
    23701 caaccaccgg tatgtagtaa gccagtatct cgaccaccgt tgccgttcca agaatggcaa
    23761 tcgaaggcca tgatgacagc cactggaagc tgtcgttcag tggcagtatt ccggttttgg
    23821 ctgcgatggc agcaaccagc attggtacaa acaccctgaa gccggcgctg gccgccagtc
    23881 ctatgcctat tgcaacagca gtgataagtt cagcattcat tgtttctccg gttaattatt
    23941 ctcttacaat tttaagtctg gtggtttttc cgccggcagt cagctcaagg aggtatacac
    24001 ccttgcccat ccgggtcata tttatgatct ggtcatttgt tccgctgtga accagccggc
    24061 cgttcagatc aaagagcctg gctgaatact ctgtctcact gataatatgc aggatgtcac
    24121 tgacaggatt gggatagacc atgattgcat cctgcccggt ctcctcaacc gccgaagctg
    24181 cagatacgat gatgtagcca ttccttgtgc tggtgttgct gccctggtta ttggatgcag
    24241 ccagtgagac cctgaatgtg ccgggagtat tatagctgat gaccggattc tgttccgttg
    24301 aagatgcagg tgttcctcct tcaaaggtcc acgcccacga ggagggctga ttggccgaaa
    24361 ggtcaaagaa cttcacccgg agaggtgcgt ttccggtctt gatgtcaacg gtaaagtcgg
    24421 ccacgggcgg gagagacaac gctctttcac cggtggcaaa gtaaacaggc catctggcat
    24481 cctgaatata ccctgattca gtcccggcgg gagaaatctt gtcagcgccc aatccgatgg
    24541 tatatatgct ctgcgacagc ccgtccgcct tcgtgaaggc aattctgtca tcgttctttg
    24601 agaaggaggg ataaccgagt gtattgttca tggttatgag attatcgagc ccggcattaa
    24661 gatcagcagc caggatggca tattgctcgt tagcagcatc aatataatcg aatgcgatga
    24721 tccagggtga attttttgag aagaccggat tgcctatgct gatatcctcc ggcagggagc
    24781 cgtataattt ggaaatggta ccatcacccc agtcattttc attgttgtcc caaacgttga
    24841 tgatcccgat atcccagtat ccgatgtctt ctccggtgga ggatgtgagt tcgttatagg
    24901 agtcatagac aaggtactgg ccggttatgt cgaattcaac agcatcggca tacagtacgc
    24961 cgcctgccgt ggtgttgtaa tggcttgtcg tgggattgta cagcttgaag acagtgtcac
    25021 ggaccactgc ccccgaaatg tcaaaaagat aaattgatgt gtcagcgtag atgctgattg
    25081 ctgctatcct ctttccgtct tttgaaatgg caacattatc ccagacctgc tgaccggaga
    25141 tcataaactc accgggatcg gaagcattat ctggtgataa tcccctcagt ttgctgtcgg
    25201 tcgatacaaa gacagcaagt gaaccatcat cagtcacact gaccttacct ttcatctccg
    25261 ttgaggtaag gggtgcgtcc tccgtgccgg atgtcctgta taatgttccg gagaaggagg
    25321 agctggtatt gtagttcagg aggtactcct gcccggcatt tacggtataa tcctgtatct
    25381 tatccacctg ttcctcctgc tggatgccta ctgccgtaaa cgcttcctct gccttcgcga
    25441 cttcggttga tgatgccccg tacaggtcag tggctgcctg tacaacagcg atcctgaaat
    25501 cgatgaactt tgaggtcatg gtcaggtaat cggtcagcgc cttgtagaat acctgttcag
    25561 ccttttcctt tgtcacggcg gtggcataga gataataggc atgattcccg ataccgctgt
    25621 ttatatgaac tccgccatta tcctcgctgc cggtgtacat ctcggaaaca cttttcggct
    25681 gccagtacgg atggctgaga gatgtgccac cgttgtgagg gtcagccatg tttctcagcg
    25741 ctcccgaagg ggagtaagat gttttggtta tgtcttcgcc tatgcgccag tcgtccctgt
    25801 cgaccatcga accgaagata tcggcgtacg tttcgttaat ggcgcctgac tgcccgtaat
    25861 attcaagatt cgcagtattt gataccacac cgtgaccgag ttcatgagcg gctacatcaa
    25921 gcgctcctgc cagtgagtag aagcttgtgc caccgttgcc gtagaagaca gccttgccgt
    25981 tccagaatgc attttccatg gaggtgccgt catcctcagc cacattaacg agagagatga
    26041 tgtcaccgcc gcgcccgttg atcgagttgc ggccgaatgt gttccagaaa tacctgtaag
    26101 ccagtgcggc atggttgtgc gccgagaccg cgcttctgac gggccatgcg ttgtttgacg
    26161 aagtgatgta gctgtaatca agatcagttg tggaggtgtt gtttgcgtcc agggtcatta
    26221 tgactccctc gtcagtggcc ccgttgaaca tatgttcaga aatattaata aggtaatacg
    26281 ttcctgactc caggtacacg ctgattgtct gaagtatgtt gttaaggtca tatgcctggg
    26341 cgggcacagc cccgtcagag catgtgctgt taaatttatg gatgatctca cctcctgcgg
    26401 catcaatgaa gtatttccat tcctcgatga agttgggtct tattgtcacg gcccatgcca
    26461 gcctgtactt atcctcacct gttgaataaa gcacaaggga tgttgaggga gctgaataat
    26521 caagtatttc ctgctctttc tgagagagct ttctgtatac tgtctcagcg ctgacatcct
    26581 ctgtcgcctt cctgacggcg gtggcggcac tcactgaagg agttgtcttt gtattcctgt
    26641 caggcctgtg aaaagctccc gtgaaccgct cccttgcgcc atcgagatga acggttgcct
    26701 cagagccgta gatatccact cccctgaact gctgaacggt acggatatgt gtgatgccaa
    26761 gcctgtctgt actgattccg gtaatcctga aggactcccc cgggttttcg accccggtca
    26821 gtgcttttgt ctcatcgaga aacatgtaga acctgtcctc cggtgaggcc tttgtctccc
    26881 tgctttcttt ttcaataaat accggacggc ccctatgcct gattatctga gttgaacgct
    26941 ctttgtgacc tgataatacg ggttgcacaa gagtcctgat ctgaggagta cctgattggg
    27001 caacagtcct gcccggagag actggctgcc ggggtatgac cgggagatct atcaccggtg
    27061 tgccgttctt tttttccttg attttgaatg gattagcctg cccgctggct gccagtggcg
    27121 gaaagaccat tagcagtata acaaggtatt tgttcattgt tgttctttta ccaggttcat
    27181 taatctgaca taaagctcct taagctgagg attacggtca tgatctgacc actgcaccgc
    27241 gttccgtgag tcaccacgca caacacttac ataccaggtc atgtttccgg gttcgtcagt
    27301 ttcaactgac atgaagttca ttgcatcaag ggagacttcg atttccctta cctcttcccg
    27361 gctgatcttt cctgtcctga tcacactgtc atttttcagt ctgcagatac ttccgttttc
    27421 gaaaaggaca taatcggtac tcatatttgt gaatccgccc ttgttgccga aaaagatccg
    27481 gtcacccctg ttgctgttca ggagtttttc aggagatgag caggaggtag ccattatcat
    27541 gcaaaatatt aatattctgt acattggacg agaaattata ttaaatgaaa tatccattat
    27601 cctaaaggtt tacagtttaa tgacgtacgg tcaataaacc gaaagctaaa tatttcaatc
    27661 agtaattcct aaggttattt acacggcatt tcagagtttt cgtgaagcgc cctgatcagg
    27721 gcctcctcga aattgtaaaa cgagcctgat gagcctgctt ccgggcctcc ggcaataccg
    27781 ttggtgataa agatgaatcc gtattttttc ttcggatcga agtagaagtc tgagactaat
    27841 ccgtaagctg atcccgagtg tcccaggaaa tgagaacctt cagggaggat gctgccggag
    27901 gccgatccgg tcagtatctg aacactcagt ccgcgattgc ctgcccgcac cccttcattg
    27961 tcactgttgg cgggtgtggt tatgaattgt gcccggtgca tcagcctgat actcttcccg
    28021 gaaatgatac gcttgccttc atattttcct ttattcatgt gcagtatcat aatgcgtgcc
    28081 agttctgcgg cggagatcct caggccgccc tgcggcgaaa agactgcgcc gttcgttccc
    28141 gtgacataac ccgagaagtc gcgtggtgac gacatcatcc ctttgtagtc atcagttccc
    28201 ggaatccatt taccatcctc cctgccgtaa attaccgcaa ggttgttgat gtcagtcacc
    28261 ccttcggcga tatatgatcc gctgatgccc agcggtataa agagagtctg ctggatatac
    28321 tcctcaaaac gctgtcctgt tatcctctct atgattgtgc ctgccagtcc gaagttggca
    28381 ttgcagtagg tataatattc tccgggagct tcctttctcc acatgtcgtc agtgtaatat
    28441 ttgcctccgg gcgcgaacag ctcgctgaat gaggggggat ttcccacatt gttgtaggtt
    28501 gccatcagga aaggatcata acctgatccc tcattcagtg atccggtatg cagcagcagc
    28561 atccttacag tcacgggctt ctccggatgg ttgggattcc tcagcctgaa tccgaggtaa
    28621 tcaccggcat cagcgtcaag gtccagcctc ttctgatcgc aaagtttcat catggccgtc
    28681 gtcataacga atttggagat ggaggctatc cggtatttgg tgttttcgtc caccgggaga
    28741 ttgcggtcat agtccctcag acctgtgtac cactctcctg tgatcttgcc gtcacacaca
    28801 gcaaccacgg atagtcccat caggccatat tcacctgcta ttctttcaat tgcctgctgc
    28861 atctgtccgt tcattgactg cagggagatg gccaggataa tggaaagtac tgcttttttc
    28921 atatcgttgc attatttagt ttccggtaac tgaaaatcta cgtacagacc tgccttcgcc
    28981 ggtgtaaagc acgcagaagt agataccggg agccagcttt tctgcatcaa tttctgatgt
    29041 catcataccc ggagggaggg agccctgttc ggtcacttcc acaagtcttc cgcctgagtc
    29101 ataaattgca atgctcatgg cgcagggacg ggacagataa aagctgacca gcgccttatc
    29161 ctttaccggg ttcgggttta ggctcagtat ctcgctgcct cctgttcccg gcattgccgg
    29221 gctgccgtcc gaagagactg ttccttcgat cactgccttc attccgttcc acagttcatg
    29281 tcttccggcc tcgtagctaa gcgcccatat gcccactccg gccaggttct ttttttttgc
    29341 gaagtctgtt ttcaggagca ggctgaggct gtcttcgaac cacatttgtc tccaccgggt
    29401 gtcattctgg taactcatcc atggcacgcc tgtgtcgttg tcgaaggctt ttccgttagc
    29461 gtttaccatc ggtatggttg caatgtatgt ccgtgatgaa cctgtcccgg tggttccggc
    29521 ctttctgaag ctgctgctta ccggccagtc atagccgtac catggcatgc cgagcagcag
    29581 cctggaggca gggactccgg catccaggta gtcttcctgg atgctcctgg tgatgttgta
    29641 gttttctccc cgcaggggag ccacgggacc tgcggtggtg gaaccgctcc agtaataatt
    29701 gtagcccatc atgatcaggt agtcacagat gtctgcaagc gcgttcaggt cccatgcgtc
    29761 agaccagttt accgcaggtg ttgccaggga gatctcagcc tgcggcagct cagccttgat
    29821 gcctcggaca gcccttctgc aaaagcttac catattcgcc ttcatggaag cagggacgga
    29881 ttcaaaatca aaatttacac catcgccgtt gcgcatcttc agctggtaga tcagggtatt
    29941 tataaggtgc cgctgtttaa cggtatctgt gagcagggca atattcctgt cagagccgaa
    30001 gttggtaacg gtgagcataa cctttacacc ctgctggtgc gcatagtcta tcaccggggt
    30061 gatgtcccaa cctctaaggg ttgtatatga tccggtttca gtgtctacct cgtaactgaa
    30121 ataggcaata tgggtcagca ctgaataatc atagtgaagg aatgcggtat cgcttgccca
    30181 gtacgggtgc cagccataaa ccaggaaggc aggctccccc tctttcctgc tcttgagtgt
    30241 caccggtgtc ccgggggaag caattgagcg aaggctggca gtgtcaaaga ccccggaata
    30301 gtattggctt tcggcctgat gtatgccgat aaacaggctg tcaggttcct gggcggcaac
    30361 cggtaagcag gtcagaacgg caattactgc tgcaagtgtt atcctgcgcg gcatgcagac
    30421 gattgaaagg tggatcagtt ggttttaaag tactttttat agatctccat gttccgttcc
    30481 agcagaacca cggaaacttc atacgccttc tgggaatcct tctccttcag cgcttccaga
    30541 agctcgctct ggtatttcat ggtataatcc ttttcaccct caatgttgcc gtaaatgaga
    30601 ttcctcatcc ggggcaggag ggagtaaacg ggttccaggc ttatgatcac tatcgggttg
    30661 cctgtggcct ttgacaggtt catgtggaac cggttgataa ggtcaacctc aagctgggtg
    30721 ttgtcaggat tgcatttctc cagttcatgc tgatttttct gcagggtgcg gatgtcctca
    30781 tccgtacggt ttcgagcggc cagcctggcc acttcgggct cgaagagcct gcgaacctca
    30841 attatctgca ggatgaggtc agagttgaac ctgaggtcat agaacagatg gagtgaattg
    30901 atggcatcct cgattttaag ctcagagata tacatcccgc ttcccttgcg gatgtcgatg
    30961 agtccgcgtg cgctgagacg cctgagggct tccctcaggg ctgtccggct cacggcaaac
    31021 tgagtgcaga gctctttttc ggttggcagc ttggagccgg gcaacagctt cttctgtcgt
    31081 atcgcttctt cgattcgtct ctcaatcttc tgactgagtg tcagccccga accgatttta
    31141 ctgaagacat catttgtcat tttacctata gttttccctg taaaactcca gggctttttt
    31201 caccgatccc tgctccataa ggagtatgcg tgcagcttcc ctttgaatat taagctcctc
    31261 cacaatcatt ctcgtgcctc tttcaatcag tttcttgttg gtcagctgca tgttgaccat
    31321 cttgttgccc ttcacccttc cgagcttgat catgagggtg gttgtgatca tgttcagcac
    31381 catcttctgg gcagttccgg ctttcagccg ggtgcttccc gtaacgaatt caggacccac
    31441 cactaccaca atagggtatt cggccacatc cactatgggc cccttggggt tacaggtgat
    31501 gcatccggtc agaatgccat tctctctcgc cttctgcagt ccgccgatca cgtacggggt
    31561 ggttcccgag gcagcgatgc caatgaccga gtccttcgtg ttgatattga atatttcaag
    31621 ctcgctccat gctttcgtgg tgtcgtcttc agctgactcc actgccttgc gaagggcagt
    31681 ttcgccaccg gcgatgagac cgatcaccac cgtatcggga acgccgaatg ttggaggtat
    31741 ttcggaggca tcaagcaccc ccagccttcc gctggtgccc gcgccaagat aaaagaggcg
    31801 gcctcccttc tgcattctga catgtatctg gttaaccaga ttctctatgt cagggatggc
    31861 gttctttacc gctacatgaa cctttgcatc ctcgtggttg atattggtaa gcaactcatt
    31921 gacagtcatc ttgtcaagat cctcaaaatt ggacggcgac tccgtaatgc tcaggtcctg
    31981 ttttttctca gcctgtctga tcatattatt atttatctga attctgaata tggtacttca
    32041 ccaggttttc catgggagta agggtgaaca gccccggctg gagcctgtgc ttcagcagca
    32101 actcttcgag gaacggcctg aaatgataag ctatgcttcc cgtgaagtga accgggtacc
    32161 tgcgtgcctc gggatatgaa agcacattgc ggacaatgaa ctcatcgaag cttgatgtga
    32221 tcatggcctg cagctccggt acatggatat tgtcagagat gaacctggta aactgtccca
    32281 ggaacctgtt cgggaagggc atgttgtaga cgttgtcaag aatctcggcc ggagtgaagg
    32341 gataagccct gaaaaagttc tcgatcacta tgcccggcag ctgcttcttg agcactcccg
    32401 ccaccagctt cctccctatg acagctccgc cgccctcatc accaaggata taacccagtg
    32461 gcgagacatt ggcaacgatc tcattaccat tgtaataaca ggagttggag cctgtgccta
    32521 tgatacaggc tatcccgggt tcattctggc acagcgacct ggctgcaccg agcagatcac
    32581 tgccgatgaa cagcttgtcg gtgccgaaaa atgaagacag agctgccctg acaatatcag
    32641 ccttggaagc attgcttacc ccggtaccgt aaaagaatat ccggctgaca cccgctccct
    32701 tcaactccgg caactctttg ccgaaaagac ggatcatctc ttcacccgtc acaaaaaacg
    32761 gattgattcc tgtagagatg tatgacttcc ctgcaactcc atcgctcacc tctctccatt
    32821 cagtctttgt agagccactg tctgcgatca gtatcatctg tgtgggaaat ttgctttaat
    32881 atttctgctc agcggattac agggacaagt tacgtttttt gaccgcttcg taaccgcaaa
    32941 gtaataacaa atgtaattat ttaaattgaa ttatcagttt tttgtcagat gtatgacata
    33001 tcttaaaaaa ctttctactt ttgaatcatt attactatat tcagcaaaat ctatccggaa
    33061 atgcgaatat cacatcccct tcatttattg gtttttacaa tggcgattct gatcgctggt
    33121 cagcaatctg ccgctcagaa ggccggccgg gccgggagac ttgccgtttc gaagatggag
    33181 aaatgggtca gccctgtcgg ggaaggaact ctcccggagc gatttgtcat tgattcattg
    33241 aaggttgatg aagagaagag ggaaataaat atctggttcc ccgtggccgc ctcatacaac
    33301 ccgatcaggg aagaaaccgt caccggcctt aacagctctg taaggtctgc ccttgggaga
    33361 aaatttgccg gttacagaat caatatgatc tcaaacggct taagcctgga gagcctggtc
    33421 cccaattact tcaggaacgt gatgccggat gacggcgacc gtattatgcc tgccgcagaa
    33481 gcacctgccg ggcttatccg gagagtgaat gctgtctcgc cgtcaagggg actggaggga
    33541 aggcacattg ccctgtggca cagccacgga tattattttg atatgccgct tgacaggtgg
    33601 gagtggcaaa gggcgaagct gttcggcagc gttgaagaca tatcggtaat ggcctatgtg
    33661 ctgccttacc tgactcctat gcttgagaat gcgggcgcag ttgtgcttct gccgcgtgaa
    33721 agggatatcc aggtgaatga ggtcatagta gacaatgaca tatccacagg ggcctccggg
    33781 tttgtattac aaattccggg agaacctgaa aaggcaggaa agggatttct tctgaaggat
    33841 acccttttta atggtgacaa tccgtttatg gcgggcactt cactgaaaat cagcagcggt
    33901 tcggcactat atgttccgga gatccctgag cggggatggt atggtgtgac agtctcatat
    33961 ccgcgggtac ctggttacaa ggggaaggca atggtcaggg tcactcatac cgggggtgtc
    34021 agtgaattca ttgttgacca gtcgatcggg gggggtacgt ggctatggct cggatcattc
    34081 ctcttcggtg caggagctga tcccctgaag ggctcggtga ccatatcggg gatggatggc
    34141 tcagcagcgc tgctggatgc agttcgtttc ggcggcggta tggggaatgt tgcccgcagg
    34201 ccggcagaat caatgatatc aaaccagtgg tcgctaaata ccggaagtca gcaggaaact
    34261 gcagactcag caccgggtcc ccgtgagtac agctggaagc tgagcggcaa acccaggttc
    34321 ctcgagggag caaggtactg gcttcagtat gcgggcatgc ctgactccct ggtttacagt
    34381 cccaacaagg gaaggaacga ctataatgat gattacatgt caagggccga gtgggtcaat
    34441 tacctgctcc gcaggcctga tacaacagct tcgggaggcc cgggcatacc cgttgacctc
    34501 tcgtttgcct ttcatactga tgccggtgtt acaccggatg attcgataat agggacattg
    34561 ggaatatatt caacaataac caatggaggc ctgttccctg acggcaccag ccggctggcc
    34621 agcagggatc tcactgatat cgtacagacc caggtcgttg aagatatcag ggtgcttttc
    34681 aatcccgaat ggacccgccg tgccatgtgg gacaggtcgt attatgaggc ccggaagcct
    34741 gatgtgccag ccatgcttct tgagctttta tcgcaccaga acctggcaga tcagcgttac
    34801 ggttttgatc ccaggttccg ctttcatgta agccgggcag tctacaaggg cattctcagg
    34861 tacctggctg ataccggtgg aagggaatat gtcgttcagc cgctgcctgt ttctcatctg
    34921 gcaattgagc cggttgaagg caggcgggtc accatccggt ggcagccggt atccgatccg
    34981 ctggagtcaa ccgcagctcc ggtttcatac agggtctaca tgcgcagtgg cgatgacgga
    35041 tttgacaacg gcacccctgt ttcaggaacc gcttttgttg ctgaactgcc tgcttacaac
    35101 actgtgtaca gcttccgcgt cacggcagta aatgagggag gcgaaagctt cccgtcagag
    35161 gagctggccg ttggaatcaa tcctgcggct gatggcgctg tcctgatagt aaatggattt
    35221 gatcgcatat cgggccccgc atggtttgac cgtgacggca tggccggggt ggcatggtgg
    35281 gatgatcgtg gtgtggctga cagatacagt ttcattagca caggcgacca gtatgatttt
    35341 gaacggatct cgccatggac cgatgatgat aatgccggct ggggcgcttc atactcaaat
    35401 gacgagggaa gggttattcc gggaaacacg tttgatttca ctcgcgttca cggtgagtca
    35461 gtgatggctg ccgggaaatc attcttctcg gtcagtgatg aggtgtttac aggtcaagat
    35521 tttaatctgt ccggatggtg tgcggttgat ctcctgttcg gggaggaaaa gacaaccaca
    35581 tcggcatact ggcctgacag gaaggatttc cggatctaca ccccagaatt cctgcgtgcc
    35641 cttgagcgga tgcagaaggc atcactcccc gtattcatgt caggttcata ccccggcact
    35701 gacctggtca tgactaatga cactgcagtt gcatcgctgg tgaagagaac cctgcatttc
    35761 atgccccgga ccggtcatgc agtaagaaca ggcagcgtgg ctgccaccga taaggcagca
    35821 cctgttttca gcggccggtt tgaattcaat gccggcattt ccgataagat ttatgctgcc
    35881 gaagcacccg atgccattga gcctgccgac aagcagtcag tcactgcctt caggtacctc
    35941 gaaaacaaca cgtctgcagc tgtgatgtat ccgggtgatg cgagaacctt cgtcatggga
    36001 ttccccttcg agaccatcat cagccggaaa gaccgtgatg accttatgaa acagatactt
    36061 gactttttac tgaaataaca gatatgccat ggataaatcc gtaacctgtt tccttccctt
    36121 ttccgatgcc atgtcggtga ctgctacaat ttcgtcgctg agatccccgg gcctcgtcag
    36181 caaagtgttt gttgtacttc ctccggtgag caaaggactt ccggaggggg cagaggccgg
    36241 gctgatcacc tcggaaggat tcgctgcaac agatgccctc aggaaaatgg cggcaactgc
    36301 tgctgacagt gactatgttc tcacttacag caagggattt ccacttgaca tgggaagcca
    36361 ctcgctggcc aggatgatgc agatatgtga cgacaccggt gcgggaatgg tttattccga
    36421 ttattacgag aagaagaacg atgtcatgtc tccccatccc gtcatcgatt accaggaggg
    36481 ggctcttcgt gacgacttca acttcgggtc agtgatacta tacagcggga aggcgttcag
    36541 ggatgcctgc gaccgcatgg accaggagta cagttttgcc ggcctttacg acctgaggct
    36601 gaaaatatca cagaaatttc ccctgattca tatccaggaa cttctgtata cggaggttga
    36661 gacagacacc cggaaaacgg gggagaagca gtttgactat gttgaccctc gcaacagggc
    36721 tgtacaggta gagatggaga aagcctgcac tgatcacctc aggtgtattg gcggatggct
    36781 ccccccccgg ttccgtgatg tcagctttga tgatgactgt tttgggtacg atgcatcagt
    36841 ggtaattccc gtcaggaaca gggtgaaaac catcggagat gccatcaggt cggtgcttga
    36901 ccagaagacc agataccggt tcaatctcat cgtggttgac aactattcca ctgacggtac
    36961 cacagagatc atcaggtcat ttgccgggga cagccggctg gtacacatca tacccggcag
    37021 gaaggatctt ggcatcgggg ggtgctggaa cgaggcggtc ttcagtgacc actgcggcag
    37081 gtttgcaatt cagcttgaca gcgacgatct gtacatcgat gacagcgtgg tcacccgtgt
    37141 cgtggacgcc ttctatgagc agcagtgtgc aatggttgta ggttcataca ggatggtcaa
    37201 ttttgacctg gatgagattc cacccggtct gatagaccac agggagtgga ctcctgataa
    37261 cggccgcaac aatgcactga ggataaacgg cctcggggca ccgagggcct tttatacacc
    37321 cgtgctcagg aaaatcaggg tccccaatgt aagctacggg gaagattatg cagtgggcct
    37381 tgccatcagc cgccattacc ggataggacg catttacgaa ccgctctacc tgtgccgcag
    37441 gtgggatgaa aactctgatg cggtacttga tgtggcaaag gcaaatgccc acaatcttta
    37501 caaggacaga atacggacaa ttgagctgct ggcccggaaa aggatgctgg ccggcagtcg
    37561 aaaacaggat ttgaatgact gattattcgc agaaggtaag ggatctgttc gaggggcagt
    37621 tcagggaatg gactctggcc cgtgacaatt attcccggct tgacggggtg atggtacgca
    37681 ctgtctcttt ccccggctat gatatttccg tacagtttaa tccgggccgt atcacctcct
    37741 cggcggcaaa agttgatgca aggtccatcg aggcgaggtc atgttttctc tgtgagaaga
    37801 accggccccg ggagcagagg ggcgttccct tcggggatga ctacataatc ctgatcaatc
    37861 cttttccgat tttccgtcgt catctgacta tcgtttcacg atgtcacacc ccgcaacgga
    37921 ttgccggaag cttttatata atgctcagcc tggcgcagac gctccctgat tatgtcatct
    37981 tttacaacgg accgcagtgc ggcgcatcag ctccggatca cctccatttt caggctggca
    38041 acagggggtt cctgccgatt gagaaggatg cagtaaatcc ctcactctgc agggtggttg
    38101 ctgaaaagaa tggtacggat ttgttgctct ggagcagtta cggaaggggc attctgactc
    38161 tccgtggcag tgacgctgaa gccctgagcg cagctttcgc ccggttccat gaccggttcg
    38221 ccctttcgga gcctgaccgg cccgaaccta tgcttaacat cctggcttac catgatgaag
    38281 ggaggtggac ggtgcacatc atccccagga gagtgcacag gccacggtgc tacttcgccg
    38341 aaggcgacag caggatactg ctcagcccgg catcggttga tctgggggga gtcttcatca
    38401 cccccagggg tgaggacttc gaaaagatat cagctgaaga tattacgaac attctcgctg
    38461 aggtatgcct gggggaagat gaattgaaaa ccctgacaaa ggaaatacta tgactggaaa
    38521 aagtgaacct gagatcagcg tagggattgt caccgggagc ggcctgacat tcacccttaa
    38581 cggcgatttc cttgccggcg acagcgggaa gatgctgtct ggtatgtaca ctgccgttgc
    38641 aggcgccgac gggctgctcc tgcagagtga ggccgggtcc gtcagggccg gcgatgagat
    38701 caccttcaca ccgtcggact tcgggagttg cagcttcacc cttcatgccg taaccatcgg
    38761 cgtcaatttt cactggcaga ggaatgagga ccagaccttc aggggtaacc tgaaattcat
    38821 attacgcggc ggacaggtga cagcggtgaa tattcttccc gttgaggagt acctggtgag
    38881 cgtcatatca tcggagatga gcgccacctc gtcatccgag cttctgaagg ctcatgccgt
    38941 catctccagg agctggctgc ttgcccagat ggacaagacc ctgcaactga gcgacaccgg
    39001 agaaaaatac cgcaccagct ttatcacgga cgatgaaatc accaggtggt atgacaggga
    39061 agaccacagg gattttgacg tttgcgctga cgaccattgc cagaggtacc aggggatcac
    39121 aagggcatcg actgacgctg ttgaagaggc tgtgcggatt acaggcggcg aggttctgat
    39181 gtacggggat ttcatttgcg acgcccgtta ttcaaagtgc tgcggcggtg caactgaact
    39241 cttcgaaaat gtatgggagc cggtaaacca tccgtatctc cggaaggtta tcgatgccgg
    39301 aagcggagcc aggaccactg acagtgacct cactgacgaa aagaacgcaa ccgggtggat
    39361 aaccggaaat ccggaggcct tctgcaatac caccgacagg catgtcctct ctcaggtgct
    39421 caacaactac gatcaggaga ccaatgactt tttccgttgg aaggtaacct accgtcagga
    39481 tgagctcagt gaacttgtca gggagaggac aggaattaac ttcgggcgga tcacatcgct
    39541 tgaacctctt gacaggggga cttcgggccg catcacacgg ctcaggatca caggtgacct
    39601 gaaaagtatg gtcatcggca aggagctgga aatcaggaag gcgctttcca aaacacatct
    39661 ttacagctcc gcctttatag tggaacaatc tgaagagaat ggagagatcc tctttgtgct
    39721 gaaaggtgcc gggtggggtc atggggtggg gctgtgccag atcggagctg cggtcatggg
    39781 cgcaaccgga tacacctaca ggcagattct ctttcattac ttcacgggct cagaaatcaa
    39841 aaaactctac tgagatgaaa agagttgctc tcacactcgt gcttgcggta ctggccactg
    39901 gcgctgccaa agctgactca tggataagga tcaatcagct gggttacctt ccgggctcgg
    39961 tcaaggtggc cgtgtatctt tcacaggtgc cggtagcgga aaaatcattc acggtatatg
    40021 atgcattcac tgacaaacct gttttccgtg gtgctgccga gatctgtgac ggagccgtct
    40081 ggggaatggc cgctgccgcc aggcttgact tttcagggct gacaacaccc ggaggatact
    40141 atataattta cagccgtaca aagtcggaaa cctttcgtct cggggcaaat gtatatgacg
    40201 gaacagctga cttcatacta aattacatca ggcagcagcg ttgcgggtat aatccctttc
    40261 ttgctgattc atgtcaccgt tatgacggga tgatagtgga tcacccgaca ctatcaggca
    40321 ccccgataga tgtccgcggc ggttatcacg atgccagtga tcacctgagg tacactgcca
    40381 ctaccgccaa ctcggtatac cagatgatgt tcgcctatat gatgtcgccg ggaatatatg
    40441 gcgacaatta tgatgctgcc ggagtggcag gcagcaatgg catacctgac attctcgacg
    40501 aggtgaaatg gggtctggag tggcttctga agatgaatcc cgacagcggc atgatgttca
    40561 accaggtggc tgatgaccgt gatcacaggg gtttccggct gcccaacctt gatactacag
    40621 gttacggcct cgggccttac aggcccgttt acttcgtgac cggcaggcct caggggctgg
    40681 ccgcgttcaa gaaccgcacc gagggggttg cctccactgc cggtaagttt gcatcggtat
    40741 ttgctatggg cgccaggctc ttcgcccaga gtgatcccct ttttgccagg acaatggaaa
    40801 ccagggcggc cgacgctttt gagtttgccc acacagaccc gggggccacc cagaccgcct
    40861 gcaacgtctc tccttacttc tatgaggaag acaattatgt tgatgatatg gagcttgcgg
    40921 catgggagct gtgggaacta accggcaaca ggtatttcct tgagcaggcc gactactggg
    40981 gaaccctcga gccctttact ccctggattg agaaggatac tgcaagacac taccagttct
    41041 atccgtttgt caacctgggt catgcaagcc tcggtctctc cggcacggag tactcagtta
    41101 agtatactga gttcatgagc aagggactgg acatcatcaa ttcaagaaca agggatgatg
    41161 tcttccaggt aaggataccg tttgtgtggt gttcgaataa ttatgtcgcg gctgctttga
    41221 cccagtgcag actctaccgt gaggcaaccg gggatgaccg ttttgactac atggaggctg
    41281 ccatgcgcga ctggctcttc ggatgcaatc cctggggcac atcaatgatc tgcggattgc
    41341 cggcaggcgg agactacccc ctgtatcccc actcggcagt gacggttttc ctcggaaaga
    41401 caaccaccgg cgggcttgtt gacggtccga taagcaaggc aatccatgat aacctcaggg
    41461 gactgaccct tctcaggcct gatccctatg ctgcattcca gggaggtcgg accgtctatc
    41521 atgatgatat tggtgattac agcaccaacg agccgactct tgacgggacg gcaagcctca
    41581 gctattatct ctcaacactt gagaaggagg ggagagcaga aagggccgcg tcagcatctg
    41641 atgtttatga tgggcatgga gctataataa ggagagaagc atgggagaag agcatccacc
    41701 tggtcttttc tgccgatgag tacggagagg ggcttgagca catactcaat gtactggaag
    41761 acaagaatat cagggggtca tttttcctta ccgggaattt cctgaggaac aggagatttg
    41821 attccacggt aaagaggatg atagcggagg ggcattacgt gggtccccat tcagacgggc
    41881 atcttctcta tattccgtgg gagaaccgtg acaccctgct ggtgacaaag caacagttca
    41941 atgatgacct gaaacgaaat atcgcagcac tcaggaaggc agggctgaag aaggaggatc
    42001 cccgctggtt cctcgcgccg tatgaatggt acaacagcgc aatcagccgg tggacagaag
    42061 atctgggaat gaagcttgtc aatttcaccc ccggaaccgg gaccaacgcc gactacacca
    42121 ctcctgatat ggaaaattac cgttcatcgg acctgctcat tcagaggctg gcctccttcg
    42181 cggagagttc acctcagggg ctgaacgggg caataatcct gatccacccg ggcacggcgc
    42241 cggaacgcac tgacaagctc tggctcaggc tggcggagtt aattgattac tatacatcaa
    42301 aaggatacac attcaacagg ttatagaaca gtatggcagc acaagaaaaa acccgttcgc
    42361 catgggcgtg gataccgacc ctctatctgg ccgaaggctt accctatgtg gcagtcaaca
    42421 ttatatcggt gatcatgtac aagcggatgg gcatctccaa tgccgacatc gcactgtaca
    42481 ccagcctcct ttatctcccg tgggttatca agcccctgtg gagcccgctg gttgacctgc
    42541 ttaaaacaaa acgatggtgg atagtcatca cccagcttgt tcttgccctg ggctttgcca
    42601 gcattgccct gacaattccg gcgtcgttct ttttcaagat caccctggca tttttctggc
    42661 tcctggcatt cgggtcggct acccatgaca tagccgctga cggattctac atgctggggc
    42721 tcaataccaa ccagcaggca aaatatgttg gcatccgaag cacattctac cgtatagcaa
    42781 ctatcaccgg tcagggcctt ctggtaatcc ttgcgggatt ccttgaaagc tcctcgggca
    42841 gggaaccggt aactctcttc atcgaggctt caccacagta ttcgaaaact attctgcaga
    42901 tgccacatgt gacggaaaat gcggcaaagc ccggtgacct gtattttata cctgacagtg
    42961 attcacttct ggtcagcact cagactgtcg acagggacag cctgggcata ctgctcgact
    43021 caatcaaatc ctccaacctc ggcaacggct tctataagct tgaagagaag atggctaagg
    43081 aaaagggttg gtgggtcagt aatatttcag atcccctggg agcctggatc agggaaaact
    43141 tcggagagga gaggggcgct gcagcctctg ttacaggaag cgtggcggtg gtccccgtca
    43201 ggattagcgg gaaacctgct cacgggaaag aggtggttct caacactgcc ctgaaaaagg
    43261 gagataaaag cattgccctt atccagggcg aacacctggt cttcaacgaa acaaactggg
    43321 acaaacctgc ttacatggtg ttccaggctg actcaaagct taccggctat acttcagtaa
    43381 catatgaggg tctgtcagga aacatcccga tggcctgggc agtcaccttc tttgtgcttg
    43441 ccgggttatt ccttgtcctg agtttctatc acaggttcgt tctcccgaaa cctgactctg
    43501 acaggccgca tgaaaacctg aaggcctccg atgtgctgaa ggagttcgga gccacttttg
    43561 cttccttctt caggaaaccg caggtgggcg ccgcgctgtt cttcatgctg acattccgat
    43621 ttgcagaagc tcagttgctt aagcttatca acccgttcct gcttgacccg atagacaagg
    43681 gagggctggg actgacaacc ggcgaggtcg gctttgtnnn nnnnnnnnnn nnnnnnnnnn
    43741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    43801 nnnnnnnnnn nnnnnnnggg aaaaaggctc ccagataata tgaattatgc caacggtaca
    43861 tgaagaagga tttcattttt gaccccctca ggaaagagct cgacggcgat ctttatactg
    43921 atgacattca gcggatcctg tatgccactg atgcctcagc ctacagggag atacctgtgg
    43981 ctgtcaccag gccggcgtcg gtctccgata tcaggaagat cctggcattt gcacgaggca
    44041 cgggcggctc cgtgataccg agaggcgcag gcacctccct ggccggccag gttgtaggcc
    44101 cggggattgt tatggatatt tcgaggtatc tcaaccatat cctggagttt aatcccgctg
    44161 agagatgggt gagagttgaa cccggagtgg ttctcgctga gcttaaccgt tacctggctc
    44221 cccacaggct gcagttcgga ccggagacat cgacagccga caggtgctgc atcggaggaa
    44281 tgcttggcaa caactcgtgc ggagcacatt cactcattca tggcagcgtc agggatcata
    44341 tcctcgaggt tgacgcaatt ctctcagatg gttctgaggt gcatttcggt gcgttgacca
    44401 ctgatgagtt caggaagaaa tgcagcggtg atcccggact tctcgagacg aagatctaca
    44461 ggaatcttca ggaaatgctc tccgatcagc gcaatgccgg tgagatacgc agggagtatc
    44521 cgcatcctgc cctccggagg cgcaacaatg gctatgctct tgatgccctg cttgagaccg
    44581 atccctttac cggtaacggc cagaagttca atgtctgcaa gctccttgca ggttccgaag
    44641 gcaccctggc attcaccacc tcgatgaaac tcaacctggt gccggtgaca aatgaccgga
    44701 ccggcctggt ctgtgcccac ttcggcagcc tgcaggatgc cgtcagggcc aacatcattg
    44761 cgctgaagca taacccggca gcggtggagc tgattgatga ctatatactc aactgcacga
    44821 aggacaatat cgagcagaac aggaaccgtt tctttgtcaa aggagatccg aaagccatac
    44881 tgcttattga gatcaccagg ccgacagttg aggagattga aaggacagcc gccgccatgg
    44941 aaaaagatat ggtcgcggca gggttcggat accacttccc gctgtacacc ggcgctgatg
    45001 agatggcccg ggtatgggaa ctgcgcaagg caggactggg agtgctgctc aacataccgg
    45061 gacggcgaaa gagcgtgcag gtgatcgaag acactgcagt gctgcctgat ctctttcccg
    45121 attatattgc tgaagccgca ggcatcctgg agaaacacag gctcagctgt gcctactatg
    45181 cccacatcgc caccggagaa ttgcatctca gcccgttgct cgacctcaag gatcctgatg
    45241 atatccgtac gttccacggt ctggcggaag atatggctgc cctggtgaag aaataccgcg
    45301 gctcgctcag cggtgaacat ggtgacggca ggctccgcgg cgagtttatc ccgttgatgc
    45361 tcgggcctca taactatgct cttcttaaag agctgaaaaa gacatgggac ccttccggtc
    45421 tgttcaatcc gggcaagatc accgatactc ctccgataac tgaaaacctc cgttacccgg
    45481 ctggcgagag gagacaatat ccggggttgg ttttcgagtt tcccggagag gagggtctgc
    45541 tctatgctgc cgagaaatgc agcggttcag ccgactgccg gaagagtgca ctggttggcg
    45601 gcaccatgtg ccccaccttc atggcaaccc gtgatgagga taagtcgacc cgtgccaggg
    45661 caaacattct cagggagttc ctgacaaggt cagacaggaa gaataagttt gaccacgagg
    45721 agatttatcg tgtgatggat ctatgcctgt catgcaaggc ctgcaaggct gagtgcccgt
    45781 caaatgtcga tgtggccagg ttgaaggcag agttcctgca acactactat gatatacacc
    45841 acgtcccgtt ccgcgcctgg ctgatagcct ggctgccccg tctttacgtt cccgggatga
    45901 tgttcaggcc tcttaccaac ctggtcaccg gcctgccggt attcaaaaga atcatcggtt
    45961 tttctgttaa aaggcctgtt ccggcgcttt caccggtgac actcaggacc tgggaaaggc
    46021 gtcacaggag aaagaacggg aagctgggcg cttcaggcag tgatagagca gccggccttt
    46081 caggcacctc gggaagtcag ggcagcagcc ggacggagaa cgccgggatc ccaaagggta
    46141 ccgtctgcct ctttgccgat gagtttacca actataatga aagtgatata ggcatcaagg
    46201 ctgtcatgct tctcgagaga ctcggttata aggtcattat accgcgccac gctgagagcg
    46261 gccgtacgtt cctctcgaag gggctgctcc ggtcggcacg caaaattgca gtgaagaaca
    46321 ttatgttgct ccgcggtgtt gtttccgatg aaaggccgtt gctgggcata gagccctcag
    46381 ccctgcttgc tttccgcgat gagtaccctg agatggccgg acgaacactt gctgatgatg
    46441 cccggcgcct tgccggcaat tcaatgctga ttgaggagtt catctgcagg gaagctgacc
    46501 gggggaatat atccgcggcc agctttactg accggccggc tgagatactg cttcacggac
    46561 actgtcagca gaaggctata gcctccacgg ctccgactat caggatgctt tcacttccgg
    46621 caaactacag ggtgaaggag ataaatgacg ggtgttgcgg tatggcagga gcattcgggt
    46681 atgagaagga acactatgac ctcagcatga agataggtga gatggtactg ttccctgcgg
    46741 taagggaagc cactgcggag accgtcattt cagcacctgg aacctcttgc cgccaccata
    46801 tcatggacgg tacaggaaga aaagccctgc acccggtgga ggtccttttt gatgcactta
    46861 taaaatgaat tgaaacatta aaacctttga ctatgagtac tttcgggttt agcaaggtgg
    46921 aactggcatt ctttgaacgc tcaggcagaa caaagatgac agcaagaatg ccgtatatag
    46981 ttgtggataa tttcccgggt ctggggcttc ttacctcgct caggttcctt gaatgggtgg
    47041 ccgggaaccc aaacggagta atcagcctgc ctacgggcaa gacacctgaa tatttcatca
    47101 aatggacaaa atacctgctg aataactggg acagtcccga agcccgtaag ataagggagg
    47161 acaacgggct gttcctgaag gagaagccgt cgctgagaag cctccatttc gtacagatcg
    47221 atgagtttta tccgattgac ccgcgccagc acaacagctt ttatgactat gtgcgaaatt
    47281 attatatcga gggatttgac cttgatccgg ccagggcatt gctgatcaac tcggaagaaa
    47341 tagtcctggc tgacgggaag catttttccg aggtttttcc tgacaacatc atcgacctct
    47401 cactccgcaa cagggaagcg gttggtcctc ttgagaaact gcagcaggag tcgctcttca
    47461 ggatagacaa ctggtgcacc ggttatgaaa agaagatccg tgcccttggc ggaatcggct
    47521 tctttcttgg cgggatcggt cctgacgggc acatcgcctt caacaccagg ggaagtgatc
    47581 ataactccgc caccaggctc accgcgacaa acttcgagac acaggcggcc tctgcaggtg
    47641 atcttggcgg gatcgaggtg tcaagaacac gcctggtgat tactatcggc ctgggaacca
    47701 ttacattcaa tcctgatgct gtggcaatca tcttcgccgc aggtgacgcc aaggcactgg
    47761 ttgtgaggaa tgcgcttgag aatgaaccgg acaatctcta tccggccact gtgcttcagg
    47821 gactgaaaag cgccagattc tatctcaccg ccggcgcagc agttcagttg catgactccg
    47881 ttgaaagata cttcagagag ggcgaatgga ccttcgagaa aacagaaagg gcggtcttcg
    47941 acctgtgtca gaagatagag aaatatgcac ataagcttgt tccggaggat cttaagaacg
    48001 atgaatactg ctcactgatc cccggactga gtctcaacac tgttgctgac gtgattgagt
    48061 cgacaaaacg gaagattgaa gccgggctca ggagggaacg gaacaccacc ttccttcata
    48121 ccgggccaca tcatgacgat ataatgctgg gcatcttccc ggccataatt ccgcagttgc
    48181 gggagatcac caacaagttt catttttcgg tgctcacatc gggattcacc gcggtgacaa
    48241 acaccatgct cctgggcctg cttgaggaga cacagcgtct tatctctgca ggaatgatcc
    48301 agatgctgag atatcctgat ttcttcgagt cagggtatag gttcaagtgg gacaaggatg
    48361 tgagtcatta cctcgataag gtggccgaca aggatgaagc aggcaagctc aggggcctct
    48421 gtcacaggat tgtcagggcc atggttgata tttatcgcct gaaatcaaag gatgatctga
    48481 atgaacagat aaggaaaaac attgagctga taaggaagag ctacagtggc gagaagaatc
    48541 cgcctgacat acagaggctt aagggtatgc tcagggaata cgaggaggag cttgtgtggg
    48601 cacactacgg ggtgccggtt cgtaacatag agcacatgag gctgggcttc tataccggag
    48661 atatcttcac ccagcagcct gacaggcacc gcgatgtgga gcccattctt gagtatctca
    48721 gacgtataca gccaaacatc atcagccttg catttgatcc cgagggttcc ggccccgata
    48781 cgcactacaa ggtgctccag gctattgccg aggcactcag gcagtggggc ctggagaagg
    48841 atctttcgaa tctgagggtg ataggttacc gtaacgtctg gttcagat
//
LOCUS       DAOPXU010000007        46163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_643_length_46163_cov_10.644899, whole genome shotgun sequence.
ACCESSION   DAOPXU010000007 DAOPXU010000000
VERSION     DAOPXU010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 46163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46163
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_643_length_46163_cov_10.644899"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..330
                     /locus_tag="PKK03_01365"
     CDS             25..330
                     /locus_tag="PKK03_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNX83068.1"
                     /translation="MNIFVAKLSSVTKAEDLQELFSKFGEVISAKVIMDRETGFSKRY
                     GFVEMADDRAGYEAIEKLNNSELDGSQIIVKRSEPKAEKKPFRPRRDFRDRDREPKY"
     gene            547..1959
                     /locus_tag="PKK03_01370"
     CDS             547..1959
                     /locus_tag="PKK03_01370"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNX83069.1"
                     /translation="MKNLILTLTGGNRKRLILFLLVLLLPVTIFASDGSVPKIGPIRV
                     EFIIFGLILLGVALFHKQTFWVAVTGLTVLMIFKFAFDPAFRLGEHMFGTTPFIEQIM
                     DKDLRQGEWGIILNLLGLLLGFAVLSKIFEESRVPDILPRYLPDDWKGPFLLLIFVFI
                     LSSFLDNIAAALIGGTIALVVFKKKVHIGYIAAIVAASNAGGSGSVVGDTTTTMMWID
                     GVSAFNVLHAYVAAFVALLFFAWFASHQQDRHQRIQKDADTNVRIDWVKIVSVILILA
                     GAIISNILYDMPALGVWIAIVLGAFLRPVPWKEVPGSLKGTIFLLCLVMCASMMPVEE
                     LPDASWVTAFALGALSAVFDNIPLTKLCLDQGHYDWGMLAYAVGFGGSMIWFGSSAGV
                     AITNKFHEARNVVLWLRKGWHVAVAYVLGFFALYLLLGWEPADTKEHKVINCPVPGCP
                     MAGQGEAAEGAVSYLDLINK"
     gene            complement(2068..3534)
                     /gene="nhaD"
                     /locus_tag="PKK03_01375"
     CDS             complement(2068..3534)
                     /gene="nhaD"
                     /locus_tag="PKK03_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HNX83070.1"
                     /translation="MFILMVVLFLLGYLAIALEHPIKVEKAASALLLGSILWALYALS
                     SEQVLAMGFSPSWEELKLMGQQILNNIAPAMTPDQFATSPFRETVELAGHSSTFVKHE
                     LAHHLVDIAEILFFLFGAMTIVEVIDQHQGFTVITNRIATKSKVRLIWIIGFITFFMS
                     AVLDNLTTTIVMIALLRKLISDKPTRWIFASMIVIAANAGGAWSPIGDVTTIMLWIGG
                     QITTVNIIIKVILPSLVNMIVPLAILSFMVKGDVKRPEKAHGSDEITTPFERTFILIL
                     GVSMLLFVPVFKTVTHLPPWVGMIFGLGVLWLVSEIMHRDKEDEIRKKLTIYNIVKKV
                     DTPTIFFFLGILAAVAALQSAGHLSLLAGWLDEKLGDIYLINLAIGAISAVVDNVPLV
                     AGAMGMYEVVTPDMLRMAADPAYAGFFVQDGLFWEFLAYCAGTGGSMLIIGSAAGVAA
                     MGLERIDFIWYMKKISWLALVGYLAGAGVYWLQAQVLA"
     gene            complement(3600..5000)
                     /locus_tag="PKK03_01380"
     CDS             complement(3600..5000)
                     /locus_tag="PKK03_01380"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX83071.1"
                     /translation="MKLTFYGAAREVTGSKILIETEHGKNLLLDCGQFQGKGLETEEM
                     NRALAFAPASIDHIILSHAHIDHSGLIPFLSRNGFTGSVVCTTATRDLCSIMLADSGK
                     IQENDTITFNKKRAKKGLPPVEPLYTQADAVAAMKLFISVAYDRKFRIDDFTTVKFTN
                     TGHMLGSGVVTLELKEKEGIKRLAFTGDIGRPVNRILKSPEPFPQADFLITESTYGDR
                     LHPSLAESDEELFRVVHHTCVEKRGKLIIPAFSVGRTQEIVYSLNNLYNAGRLPKVDI
                     YVDSPLSVNATNIFRMHPDCFNENVREQMLSDPDPFGFNSLFYNTSVEGSKSLNEIRG
                     PVIIISSSGMMEAGRVKHHLANSISNPANTVLVVGYCAPSTLGARIVRGETEVSIHGT
                     KYPVRADIEKIESYSGHGDYSEMADYLSCQDQGKLKKIFLVHGEYETQVRYSSYLESK
                     GFGNIEIPAQGMSYSI"
     gene            5161..5808
                     /locus_tag="PKK03_01385"
     CDS             5161..5808
                     /locus_tag="PKK03_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNX83072.1"
                     /translation="MKLDKIFSYLAPKEGKFYPLFKDASANLIIGAELLVEFMNEPSP
                     EKREILGHKIKETELVGDQIAKNLYKALSGTFITPFDREDIFELIHATDSVLDLIHGA
                     SKETHLYKLREIPSEFREMADVIRKASIEINTAFNGLRDVLNLSKYKDNCHRIGELEE
                     QADDINHRYLAKLFQEEQDAIELIKKKDILAALEKAVDRCDDVSDILSSVIIKIA"
     gene            5820..6866
                     /locus_tag="PKK03_01390"
     CDS             5820..6866
                     /locus_tag="PKK03_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNX83073.1"
                     /translation="MDLSLLIVIIVLAFAFDLINGFHDAANSIATIVSTKVLTPFQAV
                     LWAASFNWLAYLVFELNVADTIAKSVDTSAITLMVILSGILAAIIWNLTTWLLGIPSS
                     SSHTLVGGFSGAAIAYAGWDVVHTGVILKIGAFIFLAPLIGMLFAYIISISLLWLFRR
                     ANPFRLEKKLKVGQLFSSALFSLGHGGNDAQKVMGIIAAALMVYFHGVDASSVPHWAR
                     IEMVDGRMHAIPHWIVLGCYTAISLGTLMGGWRIVKTMGTKITKITPFEGVAAESAGA
                     MLLFGTEAFGIPVSTTHTITGAIMGVGLTKRMTAVRWGVTVNLLYAWILTIPVSMAIA
                     AGVYFLLSAITGLH"
     gene            complement(6874..8706)
                     /locus_tag="PKK03_01395"
     CDS             complement(6874..8706)
                     /locus_tag="PKK03_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNX83074.1"
                     /translation="MNDKHAICRTALALAFFMLTAMLSAQPRGTGHTFTQGNEEFFLD
                     GKPFRIISGEIHPGRVPEEYWRHRIRMAKAMGCNTISAYLFWNHHESEEGIYDFRTGN
                     RDIPRFITTAADEGMWVIIRPGPYVCAEWEFGGIPPYLLRTPGIKVRCMDPVYMRAAE
                     RYISRLADVLRPYLVTRGGPVLMIQIENEYGSYGNDRNYLFALKAIWERSGIDVPFFT
                     GDGPTTYMLEAGTLPGCAVGLDSGSSEKDFELARKMNPGVPVFSSETYPGWLTHWGED
                     WARPDTADLLREVRFLMDNRKSFNFYVIHGGTNFGFTAGANSGGKGYEPDLTSYDYDA
                     PIDEQGRATPKYMALRKLIGSYLPKKVKLPAIPDPVPAMSFPETDLVPFTSVWNNMPE
                     PVLSVQPKPFEAYGQDYGFMLYSTRLTGHKRGKMTVIDLHDYATVFLDGKYIGTLDRR
                     LGINSIELPQADSDFPLLEIFTEGMGRINFAHSMIDRKGITDRVILNGMTLMNWEVRG
                     FPMSDGYVESLEAAGAEMTRPGVFFRGTLKLDQTADTFIDMTNFVKGFVWVNGHNLGR
                     YWEIGPQTRLYCPASWLRNGENEIIVFDLHKTTPGPVRGFHTMN"
     gene            complement(8744..10207)
                     /gene="nrfA"
                     /locus_tag="PKK03_01400"
     CDS             complement(8744..10207)
                     /gene="nrfA"
                     /locus_tag="PKK03_01400"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase"
                     /protein_id="HNX83075.1"
                     /translation="MSITEFISKKPWAGWLLFLATALVVFLLGMLASSIVERRSEAKY
                     AYRPPTDLQPFESDNTVWGKFYPREYKSWLMTSDTSFRSKHAGSKPLDVLATDPELAV
                     LWAGYAFSKDYSQARGHYWAVEDIRNTLRTGAPMTPDAGPQPGTCWACKSPDVPRMIE
                     KMGVESFYKATWASLGPEITNSIGCADCHNPENMTLTITRPALREAFERQGKKIEEAG
                     HQEMRSLVCAQCHVEYYFKGEGKYLTFPWDKGMTVDEIESYYDSYQFADWTHSISKAP
                     MLKAQHPDYELFRMGIHGQRGVACADCHMPFVAEGGVKYTSHHVQSPLNMISSTCAVC
                     HRQSEEELMNNVYERQDKIASLRNYLEEILVSAHYEAKAAWDAGADEAAMKPVLSLIR
                     QSQWRWDFCAASHGGSFHAPIEVASILSNGIMKGLEARIELAKVLASKGVNKVIVPDI
                     SSKAGAQKAIGLDMDALIKEKETFKSQILPTWDRSAV"
     gene            complement(10228..10782)
                     /gene="nrfH"
                     /locus_tag="PKK03_01405"
     CDS             complement(10228..10782)
                     /gene="nrfH"
                     /locus_tag="PKK03_01405"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HNX83076.1"
                     /translation="MSRPNKLIRFFTPPPQWRLPVIMLLGAFTGLAFYLLKISNATSY
                     LSDDPRVCINCHIMTPQFATWSHSSHRSITDCNACHVPHDSFVRKYYFKANDGLRHAT
                     IFTLRNEPQSIYIREPGKRVVQENCLRCHESLFSDGPGRPPEAMEGRRDRHCLDCHRN
                     TPHDRVNSISSTQFAITPTLKDNL"
     gene            complement(10846..11367)
                     /locus_tag="PKK03_01410"
     CDS             complement(10846..11367)
                     /locus_tag="PKK03_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvoyl-dependent arginine decarboxylase"
                     /protein_id="HNX83077.1"
                     /translation="MVKVSQNRIPNEFFVTQGSGCSEYAIHPGSYHMALHDAGISEYN
                     IMNYSSVLPATAKLVTLDEVDMPPYGSELCTIMAVANGYYGEHVSAGVIFAWMYKDDT
                     CEEKVGGLVCELAGYFDMEALEERLYTVLHDLHDRTFSKYYLGEPSVFRESLTIDKRY
                     GTALVALCFVNFI"
     gene            complement(11465..12436)
                     /locus_tag="PKK03_01415"
     CDS             complement(11465..12436)
                     /locus_tag="PKK03_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX83078.1"
                     /translation="MQKRYLLPALTGLIVVLLLLPLQFAAERPMLLLERLFRGGGYLQ
                     IAGIAIFASVMEYNMLMPSRSGWWRRFSWSLFSAVFFLQLTLGLFADKLFLMTGELHL
                     PVPALIISGPLYRGELSVMTLIFLSAVLLSGPAWCSQYCYFGAIDSAVAGKKAPSRPA
                     GHRLALKNSFLILAIAVALLLRISGAGQGFALAAGIGTGVTGILIILLISRRRGKMIH
                     CTVWCPVGTLVSYVKHINPFRMRIESTCISCQLCSSVCRYDALSPDDIARLRPGLTCT
                     LCGDCLAACRHNSITYRFPGVKPDTARKMYITVTVIIYSLVLAMARI"
     gene            12525..15422
                     /locus_tag="PKK03_01420"
     CDS             12525..15422
                     /locus_tag="PKK03_01420"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HNX83079.1"
                     /translation="MIRRLISVIFFLLPAVYDATAEVQEIGTPFIRNFSKADYRASTQ
                     NWAVAQDARGFMYFANNDGLLVYDGVQWHLYQMPNHSMVRSILMGAKGEVFIGAYNDL
                     GKMVPDAGGNLSFHSFREQIPEEYRNFDDVWNICTFRDKTVFQSYYAAYFYRDGTPMT
                     VVAAPSRFQNSYSVRGRLLFNDTGNGLMEFDGTRLVRLPGCESLTGMDIWSILPYGSG
                     NEILICTLGNGLFIHDGVKLRKWDETLGRILSRDQVFSATMIYDDYYVIGTILNGVMI
                     IDREGKIVQHINRKKGLQNNTVLDLFTDRNGNLWLALDNGIDYVTVNSPITFIRSSEG
                     FGAGYASVIHEGKLYLGTNQGLYVRNWSERETGDDFRMIPGTYGQVWYLGVHNGELLC
                     GHNNGTYLISGEKADLISRIPGGWKYMELKNHPGYLIGGTYSGMILFRWEQGTWKFVR
                     QIPGMTESFRVFEEDRQGEIWMSHGFKGIFRVKLSADLDSVTGVRFYDSSDGLPSDFY
                     LGIFRVLGQLVVVSETEIYEYSPEADRFVRSTYFNQLFNPLVAVSYLREDKEGNIWYV
                     AKNRAGVFRLKEDHSYEHVATPFLLLSGRFIHGFESFYPYSPEHVIIGTEDGFAHYSS
                     RTSSLGYEGFSVYITLGEALHHDSVFYYGRTPSSADQEISYRFRYAHNNIRFMFASPV
                     YDNAGNTEYSYRLGGYDDRWSDWSRTYYKEYSNLPDGKFTFHVRARNQLGIESKPDSL
                     DFVIEHPWYKSPVAILAYVIAGMILVLLLARFITWRIELSRRNERMKNSRIYEEKEQE
                     YKRQALESEKEIIRIRNEKLQAEMILRDKELANQAMSLVRKNEFLNQIKDELRNVING
                     CNDRSAGSKVSEILQRIDREVDNNKQREIFESAFDEVHEEFLQGVKTKYPELTPTELR
                     LCAYLKMNLPTKEIAPLLNISVRGVEICRYRIRKKMGITRDVNLTTHLLSI"
     gene            15687..18758
                     /locus_tag="PKK03_01425"
     CDS             15687..18758
                     /locus_tag="PKK03_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX83080.1"
                     /translation="MKQTIMALFFIACSLALNAQGLQIKGVVTSADDGQPVPGVSVFV
                     KGTTTGTMTDMDGYYSLGNVPMNSTLVFSFVGMKAQEVVVTESTTIDIALASDVEMMD
                     EVVVVGYGTQKKSLVTGAIAKVEGDDLRKASDMRVTQALQGKTAGVVITANSGQPGSQ
                     ISVRIRGVGTNGDAEPLYIIDGLPMSGSGTDFLNAADIESIEVLKDAASAAIYGARGA
                     NGVVLITTKTGRTDTKLTVTYDGYYGFQNPWKKMPLLNSREYIMLINEASVNAGLGPR
                     FTQAQIEGFTADTDWQDEMFYYNAPKQNHSLSFTGGSDRLEYSSSLNYFSQEGIVSKG
                     KSSFEKVGYRLNAAGTFGLFKLGGNVNLAGITSKGIATNDRYGLGLNQALNMPPVVPV
                     MFSDGTWGTSEAYGFGLQEITNPVAMASYNNSKTRTYKLIGNLYGEFDFSKVSPALKG
                     LTFRSSFGGEYAIVNTDTYTPEYYLDAMHFSNIDKAYKGLDMWSRWNFENVLTYARTF
                     DVHNLTLMAGTTAFKDMHENLWGSKNDLIFDDFGHSYLDNATDPESANAGGGYNEHTV
                     SSLFARLNYDLKNRYMLTATIRRDGSSRFGSENKYGYFPSASIGWVISREDFMSNLND
                     IFNVLKLRASWGQNGSENIGDFNYTSIIGNGAIYYFGDGKTQYNGTVPTKLANPSLKW
                     EASEQTNIGVDMTTLGSTLTVTLDYYIKKTKDWQVAAPIPILVGNGAPTINGGEVRNS
                     GFEAQVSYRKQIGQLYLGASVNGAFNKSEVLDLQNTEKVLKGGDGGFGQSELIYVKVG
                     LPMGVFFGVQTDGVFQNQTEIDSYTNSTGGKIQPNAKPGDLKFIDENGDGTISDGDRV
                     QIGSPLPDFTGGLNLNAEIYGFDFNMFLYAALGQEVYSATRRYDMNFANYTAEWLDRW
                     TGEGTSDKYPRVTFADNNLNGKTVSDFYLQDGSFVRLRNITLGYSLPQNITKAIKLSK
                     LRLYVSAENLYTFTRYTGYDPEIGGGVFDNGIDRGIYPQPRTIMTGINVTF"
     gene            18781..20391
                     /locus_tag="PKK03_01430"
     CDS             18781..20391
                     /locus_tag="PKK03_01430"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX83081.1"
                     /translation="MKKSIIYSLFFLAVMVAGSSCSKDFLELEPKTGQTEANYYKTES
                     DAFLAMTAVYDAYALQNWQFAPTMADIWSDDAFCGGSDASDMSQWQDMEMFKMEPENN
                     TSADFWSRCYAGIYRANLYLQKQDGIDWKTEGLKERYEAEVKFLRAYLYWDLVRHYGW
                     VPVMTEVLPSVEDYRNLTQSAPSEIFTLIASDLLDAVDVLPNQVTPAEKGRVTKGAVK
                     ALMARIYLYHTGMSAISGLGLTAEQWGDGTAVINKAYVQTALDQVITSGVYSLIPDYA
                     DLFDWDNQNNAEAVLEMQYSDKAKSGDWGGWNINGNFASVWLSVRNPQGDATVFPGWS
                     LAVPTWSLADEYESGDPRKYVTLYDAEEMLTDYNRAFMNTGYFNKKYMARADYQGSGG
                     DPSHNFPRNFMDIRYADVLLMAAEVWLTDNPGKAAGYLSQVRTRALGAAAEKTSITLD
                     DIYHERRVEFGGEGLRKWDLLRRGLDYAGQKINASFNIPAGTPNATDFNPRTFKPESW
                     GMFPIPASEIRNMNSGVLKQMVPAYIGL"
     gene            20415..22169
                     /locus_tag="PKK03_01435"
     CDS             20415..22169
                     /locus_tag="PKK03_01435"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX83082.1"
                     /translation="MKKIRYISRLAGILAVVLAVNACEPQMADKPEVGAAPSSDQLSF
                     TITPGSDEFNFVIENTSTVTGIASWDFGNGTKATGNSNVARYPMPGDYTVAMTLVTRG
                     GLATITKTLTQSETDFSIFTDPVYVNLTGGIDDADGKTWVLDVATPGHLGVGPLDDPT
                     AMTWWAAAPYDKDGTGLMDDEITFKLDGFAVTYDNKGVSYVKAYRANDPALASVYLNP
                     RENKGDWDVDYTTPVSGTFNIIEVSEGNYTITLNGEKPIFPCFDVGAKDNTYTIRKVE
                     ENLLELTCFESYEGWNNWHYYLIPKGYVRPTITYTVNATEGTDNEVSCSVTGYSIPAG
                     QSVTNIAWNFGDGSDEVTAGKDEVVKHVYMRKGTFTVTAKLTTSIGVLTGTKQIVLAN
                     NNSAYVPFLLDMMIVYNDLSEVQVFPLLAENCALDVVDNPEKEYPNKSSKVALFSRTG
                     NQYANAYIRLPAGFRFDLRLQQVFKVMVRGKAGDVILLKLENSDPAEPWKTGTEKTYT
                     IRKDDTWEVAEYDFNGVPGGFDWTGDMYAPNVVTDSRFNHDYYNTLRLMCNSTVADGT
                     HSFYLDELSGPHVEGIHK"
     gene            22251..24509
                     /gene="bglX"
                     /locus_tag="PKK03_01440"
     CDS             22251..24509
                     /gene="bglX"
                     /locus_tag="PKK03_01440"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HNX83083.1"
                     /translation="MKLTRLSLVLSAGLFMLITSSCDKGKSVTDGAEAKADRLVAQMT
                     LDEKIGQMSQLDLGYFMDPEQLKQAVREGRCGSLLNFLGVEQVNEIQRIAVEESRLGI
                     PLIIGRDVIHGYRTVFPIPLGMAASWNPEAVRKAFRISAVEASSDGIKWTFAPMIDIT
                     WDPRWGRIAEGCGEDPYLASTLAAAMVKGIQGDSLTDPTALAACAKHYVGYGFAEAGR
                     DYNTTYIPEPLLRNVVLKPFHAAKEAGAQTFMSAFNDLNGIPTSGNRFTLKKILREEW
                     GFDGFVVSDWASVTEMVNHGFAEDDKQAAEIAINAGVEMEMATTSYYANIKALLDEKK
                     ITMEQIDDAVRDILKVKYRLGLFDNPYTDPEAGKRILDPSFLDHARTVARQSAVLLKN
                     EDAALPLSEDKIASVAVIGPLADAPRDQLGTWCFDGRAENSVTPLKAIRDLLGDKKVN
                     YVQALAYSRDKSKANFSAAVAAARRSDAVLFFGGEEWILSGEGQSRGEINMPGAQTEL
                     LEALAATGKPVILVIMAGRPLAIGREIELSDAVLYAWHAGTMAGPAITDLLFGKESPS
                     GKLPVTFVKGAGQIPFYYYRNNTGRPASDETWTPFDSIPTVNPQSSLGYRTFHLDYGY
                     TPLLPFGYGLSYTTFKYSDLTLSSQVMKIDGTLTVRARVTNTGNMDADEIVQFYVRDR
                     VGSITRPIKELKGYNRLRISRGETTDVEFTLTAADLAFFNGRGEVTEPGKFDVWIGPS
                     SEEGLHADFILE"
     gene            24528..25367
                     /locus_tag="PKK03_01445"
     CDS             24528..25367
                     /locus_tag="PKK03_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNX83084.1"
                     /translation="MIQIGTLFLAVALLAPGCGRSEKPEEFPDKPNTGDNVLVWSDEF
                     DYNGLPDPKKWGYDTDGNATGWGNNESQYYTASRLKNAEVKDGFLSITAHREDFEGFK
                     YTSARLVTRQKGDWLYGRVEVKAKIPDGRGMWPAIWMLPTDWSYGGWPSSGEIDIMEN
                     VGYDPYKIVGTVHTEAYNHSISTQRGATTNVQSCYSDFHVYAIEWEVREIRFYVDDLL
                     YFTFTNEGTGYKTWPFDKRFHLLLNVAVGGNWGGQQGIDDTVFPRSMVIDYVRVYQKK
                     TGK"
     gene            25364..26782
                     /locus_tag="PKK03_01450"
     CDS             25364..26782
                     /locus_tag="PKK03_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HNX83085.1"
                     /translation="MKRLFVLIISILLVSCNRPPENGPGRDDGTGIMAEGWITSPQKG
                     VLFTKHSGELEFMTTANTDPAIVVDTTQRYQEIDGFGNCLTGGSAILLNKMRLAERTV
                     LLRELFSPDGNGIGLSYLRITIGASDLSDRVFTYCDLPPGETDPQMEQFSIEPEKADL
                     IPVLKEILAINPSLKILGSPWTAPLWMKTNNASVGGSLKHEWFDAYAKYFVKYIQAMK
                     DEGIVIDAITVQNEPLHGGNNPSMVMTAADQALFIRQSLGPAFAAAGIKTKIIIYDHN
                     ADRTDYPLAVLNDAGARQYIDGSAFHLYGGSIDALTAVHDAFPAKNLYFTEQWVGGPG
                     NLAEDLKWHVENLIIGATRNWCRNVIEWNLASDPSYDPHTEGGCDRCLGTVTLSGNNV
                     TRNPAYYILAHASRHVRPGSVRVSTNTLGNLPNVAFQTPGGSKVLIVVNTTPDKRSFN
                     VKFRGNTLPLSLDGGAVGTWIW"
     gene            complement(26787..28832)
                     /locus_tag="PKK03_01455"
     CDS             complement(26787..28832)
                     /locus_tag="PKK03_01455"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNX83086.1"
                     /translation="MRGSIIKYIAVHALLLLILPAKLTGQVLTGSNLPIVIISTDGSV
                     TIPNEPKVKATMKIIDRGPGQRNYVTDQDNPLYLNYDGRIGIEIRGSSSQESPKKNYG
                     FTTRMADDATNNNVSLLGMPEENDWILGGMVFDTAFIRDYFCHNLFGKMGNYASRAVY
                     CEVIINNNYRGLYMLQEKLKADDHRIDVIKIGKNDNALPAVSGGYISKADKRSGDDPL
                     AWTMYSFQHSPVEYIHDLPKPEDATAAQTSYISNVFFRLESAANNNDVSVVNGYPTLI
                     DIPSFIDFMIVNEFASNPDAYQYSTYFHKDRNGKLRAGPVWDLDLSFGNDLFQWDMDR
                     SKTNLWYFEDYFYNNGSRFWRDLFYNSQFRCYLSKRWNDLLQPEMPLNRSVREAFLDE
                     TVALISEAVARDCQRWGKQARHAERIEAIKSFMQAREEWMTVGLGSYSTCSNVAVPQL
                     TVTKIMYHPQTSTQFPDGDDLEFMEIRNTGTSTVNLSGIYFMGTGVVYQFPANTTLGP
                     GLCVILASNATIFQAKYGFSPFGQFTRHLSNSGEPLILADAFGNVIDITVYSDETPWP
                     DADGNGAYLRLKDTALDNSLPESWEASSDVITSAGETVADISAILFPNPVSDRLTVRA
                     EPEISSICVYSMGGTILLMQEAGTRETTVDMSAMSPGIYIIRIFTREGSFPRMVVKE"
     gene            29242..31683
                     /gene="thrA"
                     /locus_tag="PKK03_01460"
     CDS             29242..31683
                     /gene="thrA"
                     /locus_tag="PKK03_01460"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HNX83087.1"
                     /translation="MKVLKFGGTSVGSAGAIRTVAGILQGEEGQATAVFSALSGVTDR
                     LTRALLLASEGRLEYHSVIREIERRHLDTASELLDPQRYDGFSGMIAVHLSRLDGILE
                     GISRLGEATSRSSDLVLSFGELMSLDLIELVLEEYGADVRRVDAREMIFTRPVNGSER
                     VDYEATEKAIGKLLPCTNKIIITSGFISTSVKGYPSTLGRGGSDYTAAIIAAAVGAES
                     LEIWTDVSGIYTANPAIAPDACPIAGLTYAEALEISHFGARVIYPPTIEPVMKRNIPV
                     SIRNTFASAEKGTLISSVPTPNGSAVKAVTSVGSVCLVSLTGSGMAGVVGIAARLFTV
                     LAKSNINIILITQASSEQSICIGIDNKDGERACEAIDAGFESEIEAGRVRPALREDGF
                     AIVALVGEGMKHSVGISGQAFAALGRNGINIHAIAQGSSELNISVVVKETDAPKAVNA
                     IHQEFFRSAKRVINIFVVGTGNVGSALVKQVMDRSAWFAGEYQTEFRIVGLANTRKMI
                     IREEGITGPDWKSVMMASDQKSNLESFTGQMFSLNLPNTIFIDNTSSSDVSALYERIL
                     EKSISIVTSNKLAASSSFENYSRLKRTAVRKRVQFGFGSNVGAGLPVIHTIQNMVKTG
                     DRIQRIDAVLSGSLNYIFNCYSNGMRFSEAVLQAVENDYTEPDPLTDLRGLDIARKLL
                     ILVRESGFCLEASEIAFEDFLPGPVPEKYDAERFFTQMREFDDFFEQQREELSLRKEK
                     IRIIACYESGRASIRPVRVSAEHPFYYLEGNDNIVSVYSGRYSLRPLIIKGAGAGADV
                     TAAGIFADILSIVNS"
     gene            31685..32614
                     /locus_tag="PKK03_01465"
     CDS             31685..32614
                     /locus_tag="PKK03_01465"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNX83088.1"
                     /translation="MAGKRIRVKAPATVSNLSCGFDVIGLALDEPYDMVDLELTDNGT
                     VVIDDIRGCGTLSRDPEKNVVGAVLKAASAMAGSSAGFRISIDKGIRPGSGIGSSGAS
                     AAAAAFGANLLLGEKFTPAEMVRLAMEGERLASGSAHADNVAPALLGGITLVRSYDPL
                     DIVGLHVPPELYCVVIHPETEIKTSVARGLLDRQVPLTTAVRQWGNLAGLVAGLYRED
                     YDLIGRSLIDFVAEPKRSVLIRGFDQLKKSAMECGALGAGISGSGPSVFALCRGGDSA
                     ATVMKGMSEAMKSHGIGFDAYVSPVSRRGAQVC"
     gene            32634..33935
                     /gene="thrC"
                     /locus_tag="PKK03_01470"
     CDS             32634..33935
                     /gene="thrC"
                     /locus_tag="PKK03_01470"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNX83089.1"
                     /translation="MKYYNLKDRRVITGFREAVERGISPDMGLFMPERIPRLDDAFFR
                     QLPSMTLPEIGIEVGSLYAGDDIPRKVMRQICEEAFDFPVVIRQVDERKLVLELFHGP
                     TAAFKDFGARFMAGCFRYFSSRDDKRRVVLVATSGDTGGAIASSFLGVEGVDVIILYP
                     SGKVSSLQENQLTTLGRNITAVEVDGSFDDCQAMVKSALAGSANRTNLKLTSANSINI
                     ARLIPQSFYYYYAIGCSGNGRVLSVPSGNFGNITAAAMARRSGLGIDHLVAATNVNRV
                     VPDYLKTGIYAPAVSVATIANAMDVGNPGNFDRLRRVFDDDYGAITGSVSGYWFDDDR
                     IREHIGQEYQRTGYLLDPHGAVASLGLDAYLEHHPGSGGIFVATAHPAKFRDIVEPLT
                     GQTVEIPDSLRNFLAGKKQSVKMKAGPQNIEEYLDDIYQNE"
     gene            33959..35098
                     /locus_tag="PKK03_01475"
     CDS             33959..35098
                     /locus_tag="PKK03_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HNX83090.1"
                     /translation="MGSENREFQIFVKPVGGRCNLRCSYCYYTGHSHQEGTAGGQRMS
                     DEVLETYIKQHIQAAGGGEVFFSWHGGEPVLAGIDFYRRAVALEKRYTPPGCRIVNGI
                     QTNATLIDDAWGRFLKEEGFYVGVSLDGPEWFHDSSRLRTDGRGTFTSVMRGLEILRQ
                     HDVPHEVLCVVSNCNVHASLELYRFFRDLGIEFITFLPLVERKSRNSSEVTGRSVSAA
                     DFGRFLAEVFDGWIANDIGKIKVQVIEEALRTAFGQDHTLCIFNPVCGAVPAVEMNGD
                     FYSCDHFVDGDHLIGNIMNQSLESLLDDPRQKAFGEARKSAMPRYCLECEVLDMCNGE
                     CPRNRFINTPDGEPGLNYLCDGYRYFFNHCRPFINEVARLWKEGS"
     gene            complement(35095..35946)
                     /locus_tag="PKK03_01480"
     CDS             complement(35095..35946)
                     /locus_tag="PKK03_01480"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNX83091.1"
                     /translation="MKRKVDELYDEYRNGNVSRREFMKKLALVTGGTAAAVVILPGCN
                     GATARTAGKSAMKVVGEFITYPGASGDMRAWISIPEGKDKYPGVIVIHENRGLVPHIR
                     EVNNRMAMEGFVALAPDALSPLGGTPEDQDKGREMIGQLDREETIRNLVAAVKYLETH
                     PRTSGKVGCTGFCWGGAMTNQVAVNSPDLDAAVPYYGSTPAPEDVPKIRAKMMAHYAG
                     NDERINAGIPAFEEALKKAGIEYQIFIYEGTQHAFNNDTGERYNKEAADLAWKRTTDF
                     FKETLKS"
     gene            complement(35986..37620)
                     /locus_tag="PKK03_01485"
     CDS             complement(35986..37620)
                     /locus_tag="PKK03_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNX83092.1"
                     /translation="MSEKYSVSPSGEKFPLPKTEDYKTEFENIKKRVKQERAKGREIV
                     VVMGVGFVGAVMAAIVADTVDKKGKPSKFVIGMQRPSVRSFWKIPMLNRGVSPVKAED
                     PEVDPMIARCVKEKKTLMATYTYDVLKLADVVVVDVQCDYIKEELGNVRAGQTDMAAL
                     EASLAVIAEHIPAEALVLIETTVAPGTTEQVAYPIMKKAFSKRGIKTDPLLAHSYERV
                     MPGRNYVASIRDFWRVCSGINEEAKKRVVKFLTEVLNTEKFPLTVMDRPIESETAKIV
                     ENSYRATILAFLDEWSLFAERNGVDLVKVIKAIKMRPTHSNMIFPGPGIGGYCLPKDG
                     GLGVWAYKHILGFEDDIFRITPMAIDINDTRGLHVGQLTRDALRNMGKPIAAADVLVL
                     GAAYREDVGDTRYSGSEIVIRKLAEMGAEIRVHDPYVEHWWEFEQQDTYPAEGQSKAQ
                     FFRNQEKLKEIRIEQNLNKALKGAEAVILAVPHSVYLSLDPDRVVKTIGQPAAIIDCF
                     GILTDEKIRRYFQLGCEVKGLGRGHIQRIKEETRKS"
     gene            37877..38293
                     /locus_tag="PKK03_01490"
     CDS             37877..38293
                     /locus_tag="PKK03_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83093.1"
                     /translation="MKRKSPMNKIMLLLLPAVLIMAASCGKEKQSERFELLTAHAWTS
                     DSLLAGGVDASGTGQMLEKFRGEVTFREDGTGSFGQYTGTWMFADNETSLTIASPELS
                     FPLTTHIVELKATSLKVTFTFPAVPPVNVRMTFKPR"
     gene            38290..40542
                     /locus_tag="PKK03_01495"
     CDS             38290..40542
                     /locus_tag="PKK03_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX83094.1"
                     /translation="MTAFRNWLVFVLFLAAAGAVEGQTIVSGRVTDSQTGESLAGVYV
                     IWGKGSGTSTDNDGHFSFNSDEGTRTIVFRYIGYKSLTEVLDITAGNNLVLDIKLEQE
                     AQSLDQVVVSADRMEQKRSDLTVSMDVIRSERLFRAHITDAQELITKTPGIEVLDGQA
                     SIRGGSGFSYGVGSRVLALIDGLPMMSADAGNIKWHFLPLENLSQVEIIKGASSVLYG
                     SSALNGVINFRTADASNVPVTQFYAETGLFGAPATEEWKWWDSPRLFGSTSFSHLRKI
                     NDTDLGVGVNISSDRGYRKYNDETMGRVSVRLKHYSSRTEGLKYGINLSGGKTYKTDF
                     VLWENALTGGLIQDTATVSRLHGYYFAADPYISLGRRGKVRHDIRLRFQGAGNRFPVR
                     SENNAGSVSIYGEYQFWYRFSDMVSVTAGFSETWSDIRSDFFGDHNGLNLAGFAQAEV
                     HPVSRLKVTGGVRVEQNYLDRDPDRPVPVFRTGLNFQAAKYTFLRASFGQGYRYPSIA
                     EKHASTTLGSVRIFPSPFVRAESGWSSEAGVMQGFMIGRFSGEGDLSLFLSQSSDMIE
                     YIFGVHQDPESGDSDLGFKATNTEYSRIYGTELEMLVSRPFGKVTATVTGGYTYIHPV
                     EFNAITGRSTGVWLKYRRKHTAKIGFLAVSERFEAGLDIYARSKTLNIDNVFLSPLTR
                     EDILPGFFDYWQEHNRGYLLMDCSVGYSLSEALKLSVAVKNITNAEYMGRPGDIQPIR
                     NFSLRLGGRF"
     gene            40658..41341
                     /locus_tag="PKK03_01500"
     CDS             40658..41341
                     /locus_tag="PKK03_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83095.1"
                     /translation="MTDDNDCTTAFTDSLAGRKAIDSSAYDIWSWSLGTDIVTSYIWR
                     GARQGRGPHLQPYAGFSAGPVTGGVWGTFDFNGYREVDLYLAMELPGGFIIGLQDYWM
                     ADLPFSDFTAESGSHVLEASLGYDSEHISLNANYIINEAGGAGSVGDDLYVESRFSFE
                     YFSVFMGAGNGWHTEDGRFMVCNVGIGAEREFQVSDSFSIPVTAQICYNPDSGRAFFA
                     AGLSISTGR"
     gene            41543..42352
                     /locus_tag="PKK03_01505"
     CDS             41543..42352
                     /locus_tag="PKK03_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNX83096.1"
                     /translation="MFEFSEIQNNYFFLPLIGFVIGIFGTILGGGGGFFFIPILTLWF
                     AVPAQTAVMTSLMATLPITLVGSVSHYRRRNIDVSVAAVFIAGGLIGAVAGARITGWI
                     TESQLKVAFGIYSIMMGFGIALRSRRNKEAGKLTEGQNKRDGRSMIAKGSFYGLVAGT
                     ITGAFGTSGTMPVIAGLYSMRIPLKVIIGTSLLVVLFNSIFAISAHLFIGKVDVTLVS
                     FLTAGSVIGALGGPGILSRMKIDNKAESAAAFWYAATMIIIGILMILGGRN"
     gene            42367..43095
                     /locus_tag="PKK03_01510"
     CDS             42367..43095
                     /locus_tag="PKK03_01510"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNX83097.1"
                     /translation="MIRTIYIIILIYFALGGVGFYLINRRKTPEAARESYVKFGTYFL
                     IINLLFFSIILHTMAFRVLTVLIIIAGFTELSTLFVRSERNNVPFFVCSLIIYTVFSI
                     LFFIFGAGTRDQILFAFLVLSIFDSFSQITGQLWGHHKLVPAISPGKTWEGLLGGVLI
                     SLFSAWLLSDLARDTMPEMIVFTIGIPAFAFFGDLLSSVYKRKYKVKDFNRLIPGHGG
                     FLDRFDSLIAGGAWVALYTMIMLH"
     gene            43108..43782
                     /locus_tag="PKK03_01515"
     CDS             43108..43782
                     /locus_tag="PKK03_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83098.1"
                     /translation="MGNTIVLSFVYLAGISMLLIFNELVYRKFSPEGEITRKFAHFSS
                     VLATLPFPYFFPSHWYILVLAVLFAIVLFSTRRGKLLGSIHAIERKSIGSYLLPIAIY
                     VTFLIAELLGNKFLFILPILILAVCDPLAAILGMSNTHFNRRIRIAGITLNKTWLGTG
                     SFLVSSFVISILALYFYREVFDFKTFWLAALVAVVTMFAEMISWRGSDNLSIPLSAVL
                     VLVLCL"
     gene            43892..44491
                     /locus_tag="PKK03_01520"
     CDS             43892..44491
                     /locus_tag="PKK03_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNX83099.1"
                     /translation="MKQIIYKGENILNVPNLISLYRLLAFPVILFMALTGRENWFVIL
                     ICISLVSDIFDGSIARLFKLQTNFGAALDNLADLCTYAMALLGMFLFRWSEIRPHAWI
                     LYIFLAVFILSYLVAFIRFRKIPGLHLYSAVSCGYAQGIFFFILFVFGFYPWLFYLVV
                     VWGIIAYTEKTIVLLKLDDIKRGVKGLYWLIKKERMQAG"
     assembly_gap    44627..44726
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(44883..46142)
                     /locus_tag="PKK03_01525"
     CDS             complement(44883..46142)
                     /locus_tag="PKK03_01525"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX83100.1"
                     /translation="MSESLFQSLKDAFTDKAIDAISRNLGEDASRTKSGLTAMIPTVL
                     GGILGKSANATSAPSWWNTVADLFDGDDDDDLRLGMLGDTKFADAGKGLLGSLFGGNL
                     DSILGSLSGATGLAKDKSGKLLTTVAPMVIGFLSRWAKRKGLSFTGIIGKLLADKSAI
                     LGALPSGIGGGLLGFADKARDAVDDTARKVAGAATQKPKFNWSWLILLLGALILLWLL
                     FGRGCNRTKEKVEDQATEIVNDLDQAASDIKASIKGALNDAGDWVYDLGASIKKKLVD
                     GTEMEIGVNSVEYKILEFIEDANLPIDKTTWFTLDRLYFETGSSTLKAESNSQLDRIA
                     AIMKAYPAVTLKIGGYTDNTGSDVVNNALSSERAETAKAELVKRGVALNRLEAEGYGS
                     QHPVCPANDTPECRAMNRRIDIRVTAK"
BASE COUNT        11159 a        11020 c        12232 g        11652 t
ORIGIN      
        1 attaacagtt taatttattt tcaaatgaac atttttgtag ccaagctgag ttctgtaacc
       61 aaagcagaag accttcagga acttttcagc aaatttgggg aagtaatctc cgccaaagtt
      121 attatggacc gtgaaaccgg cttttcaaag cgttacggtt tcgttgagat ggccgatgac
      181 cgtgccggtt atgaagctat cgaaaagctt aacaactcag agctcgatgg cagtcagatc
      241 atcgtaaaga ggtctgagcc aaaggcagag aagaagcctt tcagaccccg ccgtgatttc
      301 cgcgacaggg accgtgaacc gaaatattag gaaatagaac agaagaggct gtccggtgag
      361 ggcagccttt tttagtcatt caataaaggc aggggaactc tgcctttatt tatataaggt
      421 cagcgaaagc cttaaaaaaa aatcaggaag atttcatttt gcaggttaaa atatctataa
      481 tttcgccctg acaaatttat tccatcctat ccaacctcat caacaaattc ccttaactgt
      541 ttaacgatga aaaacctgat tcttaccctg accggaggta acaggaaaag gctgattctt
      601 ttcctgcttg tcctccttct tccggtaact attttcgcat cagatggttc tgtgcccaag
      661 ataggcccga tacgcgttga gttcataatt ttcggcctga tccttctcgg ggtggcgctt
      721 tttcacaagc agacattctg ggttgcggtc acaggcctta cagttctgat gatcttcaag
      781 tttgcatttg atccggcctt caggctgggc gaacacatgt tcggcaccac accctttatt
      841 gagcagatca tggataagga tctgagacag ggcgagtggg ggatcattct gaatcttctc
      901 ggattgctgc ttggctttgc ggtcctctca aagatatttg aagaatcaag agtccctgac
      961 atccttccaa ggtacctccc ggacgactgg aagggtccct tccttctgtt aatattcgtc
     1021 tttatccttt catcattcct ggataatatt gccgctgctc tcattggagg gacgatagca
     1081 cttgttgtat tcaaaaaaaa ggtgcacatc gggtatattg ccgccattgt tgctgcaagc
     1141 aatgcaggag ggagcggaag tgtagtgggc gatacaacca ctaccatgat gtggattgac
     1201 ggtgtttctg cctttaacgt tttacatgca tacgtggctg ccttcgtcgc cctgctgttc
     1261 ttcgcgtggt ttgcatctca tcagcaggac aggcatcaga ggatacaaaa ggacgcggat
     1321 accaatgtca ggattgactg ggtcaagata gtatctgtca tcctcatcct ggcaggtgca
     1381 ataatctcca acattctcta tgacatgcct gcccttggcg tctggatagc cattgttctt
     1441 ggcgccttcc tgagaccggt tccatggaag gaggttccgg gttcactcaa ggggacaata
     1501 ttcctgttgt gtctggtgat gtgcgcatcg atgatgccgg ttgaagaact tcccgatgcc
     1561 tcatgggtga ctgcgtttgc acttggtgcc ctttcagcag tgtttgacaa tatcccgctc
     1621 acaaagctct gtcttgatca ggggcactat gactggggga tgctggccta cgccgtaggg
     1681 ttcgggggct cgatgatatg gttcggttca tctgccgggg ttgctatcac caacaagttt
     1741 cacgaggcac gcaatgtggt tctctggtta agaaaggggt ggcatgtggc tgttgcctat
     1801 gtcctggggt tctttgccct ttacctgctt ctgggatggg agccggcaga caccaaggag
     1861 cataaggtca tcaactgtcc tgtgcccgga tgcccgatgg caggtcaggg tgaggctgcg
     1921 gagggagcag tcagctacct cgacctgatc aacaaataaa aaggaatcat tcttcctgcc
     1981 ataaatgaaa aaggcaccct tccaggggtg ccattttcat ttttattatg attcgctgtc
     2041 ggggcaaaat ccgattgtac tataacctca cgccaggacc tgtgcctgaa gccagtatac
     2101 tccggcgccg gcaagatagc ctacaagtgc aagccatgaa atcttcttca tgtaccagat
     2161 gaagtcgatt ctctcgagac ccattgcggc cacaccggct gctgaaccga tgatcagcat
     2221 actgccacct gttccggcgc agtaagcgag gaattcccag aagaggccgt cctgcacgaa
     2281 gaagcctgca taagccggat ccgcagccat gcggagcatg tcgggagtga ctacctcgta
     2341 catccccatt gctcccgcca ccagcggaac attgtcaaca actgctgaaa tggcgccgat
     2401 cgccaggttg atcaggtaga tatcaccaag cttctcgtcg agccaaccgg ccagcaggct
     2461 caggtgaccg gcactctgaa gggcggccac tgctgccagt atccccagga agaagaagat
     2521 ggtgggggta tcaaccttct ttacaatgtt gtatatggta agtttcttcc gtatttcatc
     2581 ctccttgtca cggtgcatga tttctgagac aagccacagc actcccagtc cgaagatcat
     2641 acctacccat ggcgggaggt gagttacggt tttgaacacg ggaacgaaga gcagcatgga
     2701 gacgccgaga ataaggatga aggtccgttc aaacggcgtg gtgatctcat ctgagccatg
     2761 tgctttctcg ggccgcttta catctccctt gaccatgaag gacagtatgg caagtggcac
     2821 aatcatgtta acgaggctcg gcaggattac cttgatgatg atgttcaccg ttgtgatctg
     2881 tccgccaatc cagagcataa tcgttgtcac gtcgccgatg ggtgaccatg ccccgcccgc
     2941 attggcggct atcactatca tgctggcaaa gatccagcgc gtgggtttgt ctgagataag
     3001 cttcctcagt aatgctatca ttacgatagt ggttgtcagg ttgtcaagta cagctgacat
     3061 gaagaatgtg atgaaaccga tgatccagat aagccttacc ttactctttg tggcgatcct
     3121 gttggtgatg acggtaaatc cctggtgctg gtcaattacc tccactattg tcatagcgcc
     3181 aaaaaggaag aaaagaattt ctgcaatgtc aacaaggtga tgggcaagct cgtgcttcac
     3241 gaaagtgctg gaatgacctg ccagctcaac cgtttcacga aacggagagg tggcaaactg
     3301 gtcaggtgtc atcgcggggg cgatgttgtt cagaatctgc tgtcccatga gcttcagttc
     3361 ctcccaggag ggagaaaagc ccatcgcaag gacctgttca ctggagaggg cataaagggc
     3421 ccacaggatt gaacccagca aaagggcgct ggcagccttc tctactttaa taggatgctc
     3481 aagtgctatc gccaggtatc ccagcaggaa aagcacaacc attaatataa acatacttca
     3541 gatttttttt tgtgcaaaaa taggatttga ggccgacatt caaacaaatg ttgtaattat
     3601 cagattgagt atgacatacc ctgtgccgga atttctatgt tcccgaagcc ttttgactca
     3661 aggtatgagc tgtacctgac ctgtgtctca tactctccgt gaaccagaaa aattttcttc
     3721 agcttaccct gatcctggca ggagagataa tcggccatct cactgtagtc tccatgcccg
     3781 gagtatgatt ctatcttttc gatatccgcc cttacagggt atttggttcc gtggatcgag
     3841 acttcagtct cacctctgac aattctggct ccgagggtgg aaggcgcgca atagcctacg
     3901 accagtaccg tattggcggg gttgctgatg ctgtttgcca ggtggtgttt gacacggccc
     3961 gcttccatca ttccggatga ggagattatt attaccgggc ccctgatctc gttaaggctt
     4021 ttggaacctt caaccgaggt gttgtagaac aatgagttaa agccgaaggg atcaggatca
     4081 gatagcattt gctcccttac attttcattg aagcagtcgg ggtgcatccg gaagatgttc
     4141 gttgcgttga ccgagagggg gctgtcaacg tagatgtcga cctttggcag gcgaccggca
     4201 ttgtaaagat tgttgagtga atagactatc tcctgtgtcc gccccacgct gaaggcagga
     4261 atgatgagtt tgccccgttt ctcaacgcag gtatggtgaa ctactcggaa aagttcctcg
     4321 tctgactcgg ccagtgaggg atgcagcctg tcgccgtatg ttgattcggt aataaggaag
     4381 tcagcctgcg ggaatggttc cgggctttta agtatcctgt ttaccggcct gccgatgtca
     4441 cctgtgaatg caagtcgttt gataccctct ttttccttca gttccagtgt gaccactccg
     4501 cttcccagca tatgcccggt atttgtgaat ttcacggtgg tgaaatcgtc tatccggaac
     4561 ttgcggtcat aggccacgct gatgaagagc ttcattgcag ccacggcatc tgcctgggta
     4621 tagagaggct ccaccggcgg aagccctttc ttcgccctct tcttgttgaa tgtaatggta
     4681 tcattttcct gtatcttgcc tgagtcagca agcattattg agcacaggtc gcgcgttgcg
     4741 gtagtacata caaccgagcc tgtaaacccg ttccgggaaa gaaatgggat tagtcccgaa
     4801 tggtctatat gggcgtgtga cagaataata tggtcaattg aggcaggagc gaaggccagc
     4861 gccctgttca tctcctctgt ttcaagtcct ttgccctgga actggccgca atcaagcaaa
     4921 aggttcttgc catgctccgt ttcaataagt atcttgctgc ctgtcacctc cctggcagcc
     4981 ccgtaaaatg taagcttcat gccgtatgat tgatttttat gatttgctaa tatagctaaa
     5041 gttgccagaa tctcccctaa attattcttt aacatttaat gaacaatatt tcaaaactcc
     5101 tttaattagt gaagtcattg atatactttt ggcgggtggt aacatttctt acactgactc
     5161 atgaaactcg acaagatctt ttcgtacctg gcccccaagg agggcaagtt ctatccccta
     5221 ttcaaggatg catcggccaa ccttataatt ggggcagagc tgcttgtgga gttcatgaat
     5281 gagccgtcac cggagaagag agagattctt ggccataaaa taaaggagac ggagcttgtt
     5341 ggagaccaga tagccaaaaa cctttacaag gccctgagcg gaacctttat cacacctttt
     5401 gacagggagg atatttttga gctgatccat gccaccgatt ctgtgcttga tctgatccac
     5461 ggtgcatcga aggaaactca cctgtacaag ctcagggaaa taccgtcaga gttcagggag
     5521 atggctgacg tcatcaggaa ggcctccatt gagatcaaca ctgctttcaa cggattgcgt
     5581 gatgtgctca acctttcaaa atataaagac aactgccaca ggatcgggga gcttgaggag
     5641 caggccgatg acatcaacca ccgatacctg gcgaaactgt tccaggaaga gcaggatgcg
     5701 attgaactga taaaaaagaa ggatatcctg gcggcacttg aaaaggctgt cgaccgttgc
     5761 gacgacgtgt ccgacattct ttcatccgta attatcaaga tcgcgtaacc ggcctgcaga
     5821 tggatctttc acttcttatc gtcattatcg ttcttgcgtt cgcatttgac ctgataaacg
     5881 ggtttcacga cgcagcgaac tcaatagcta cgattgtttc gaccaaggtg ctcacacctt
     5941 tccaggcggt gttatgggct gcatcattca actggctcgc ataccttgtt tttgaactta
     6001 atgtggctga caccattgca aagtctgtag acacctcggc cattaccctt atggtcatat
     6061 tgtcaggcat tctggctgcc atcatctgga accttacgac ctggcttctc ggcataccgt
     6121 ccagctcgtc acacaccctt gtgggaggct tttccggtgc tgcaattgca tatgcaggat
     6181 gggatgtggt acatacaggt gtgattttga agataggggc cttcattttc ctggctccgc
     6241 ttatcgggat gttgttcgca tatatcattt ccatttcact tctctggctg ttccgtcgtg
     6301 cgaatccgtt caggctggaa aagaagctca aggtgggaca gctcttttca tcggcccttt
     6361 tcagcctcgg tcacggtgga aatgatgcac agaaggttat gggtatcatt gccgctgctc
     6421 ttatggtcta cttccatgga gttgatgcat catccgttcc tcactgggcg aggatcgaga
     6481 tggttgatgg caggatgcat gcaatacccc actggatagt gctgggttgc tacacggcaa
     6541 tctcccttgg aacgcttatg ggcggctgga ggatagtcaa gaccatggga accaagataa
     6601 cgaagatcac cccgttcgaa ggtgttgcag ccgaatcagc cggagccatg ttgctgttcg
     6661 gaaccgaagc cttcgggata cctgttagta caactcacac aattacgggt gctatcatgg
     6721 gggtaggcct gacaaaaagg atgacggctg tgagatgggg tgtaactgtc aacctgcttt
     6781 atgcctggat acttacgatt cctgtatcga tggcaattgc agccggggtg tacttcctgt
     6841 tgtcagcaat aacgggtctg cactgacctg gtatcagttc atggtatgga atccccttac
     6901 cggcccgggt gtggtcttgt gaagatcaaa cactattatt tcgttttccc cgttcctgag
     6961 ccatgaagcc ggacagtaga gccgtgtctg cggaccgatc tcccagtatc ttcccaggtt
     7021 atgtccgttg acccatacaa atccctttac aaaattggtc atgtcaataa aggtgtctgc
     7081 tgtctgatcg agcttcaggg tgcccctgaa gaatactccg ggcctggtca tttcagctcc
     7141 ggcggcctcc agcgattcaa catagccgtc actcatcggg aaaccgcgca cctcccagtt
     7201 catcagcgtc atcccgttca gtatcacacg gtcggttatt cccttgcggt caatcattga
     7261 atgggcaaag ttgattctcc ccatcccttc ggtaaatatt tccaggagcg gaaaatctga
     7321 atctgcctga ggcagctcaa ttgaattgat tccaagtcgc ctgtcaagtg tcccgatgta
     7381 ttttccgtca agaaagacgg tggcgtagtc atgcaggtca attactgtca ttttgcctct
     7441 cttgtggccc gtcagcctgg tgctgtaaag catgaatccg taatcctgcc cgtatgcttc
     7501 aaacggtttt ggctgaactg acaataccgg ttccggcatg ttgttccata cagaggtgaa
     7561 tggcaccaga tcagtttccg ggaaagacat tgccggtacc ggatccggta ttgccggcag
     7621 ttttactttt ttaggaaggt atgagccgat cagctttctg agggccatgt atttcggggt
     7681 tgctcttccc tgctcgtcta ttggtgcatc atagtcatag ctggtgaggt caggctcgta
     7741 tccctttcct cctgagttgg cccctgccgt gaacccgaag tttgttcctc cgtgtatgac
     7801 ataaaaattg aatgatttcc ggttatccat gagaaatctg acttcgcgca gcagatctgc
     7861 agtgtcgggc cttgcccagt cctctcccca gtgggtcagc catccggggt atgtttcgct
     7921 ggagaagacc gggactcccg gattcatttt acgggccagt tcaaaatcct tctccgagct
     7981 cccggagtcg agtcccacgg cacaacccgg aagtgttcct gcctcgagca tgtatgttgt
     8041 gggcccgtca cctgtgaaaa acgggacgtc aataccgctc cgttcccata tcgccttcag
     8101 ggcgaagagg taattgcggt cattgccgta gcttccgtat tcattttcaa tctggatcat
     8161 cagcaccgga cctccgcgtg tcaccaggta agggcgaagc acatcagcca gccgtgagat
     8221 gtatctttct gctgctctca tatagaccgg atccatgcag cgtactttta tgcccggagt
     8281 gcgaagcagg taaggaggta tgcccccgaa ctcccattcg gcgcagacat atggcccggg
     8341 cctgatgatg acccacattc cttcatcggc tgctgttgtg atgaacctcg ggatatcacg
     8401 attgcctgta cggaagtcat aaatgccctc ttcgctttca tggtggttcc agaagaggta
     8461 tgcggagatg gtgttgcacc ccattgcctt tgccatcctg atcctgtgtc tccagtattc
     8521 ctcaggcacc cttcccgggt gtatctcacc gctgatgatc cggaacggtt tcccgtcaag
     8581 gaagaactcc tcgtttccct gtgtaaaggt atgccctgtc ccgcgtggct gcgcactcag
     8641 catggcagtc agcatgaaga atgccagagc aagtgctgta cgacaaatcg catgcttgtc
     8701 attcatgacc tgtaagatat ttgtttacaa aatgaaatgc ttctcagact gcgctcctgt
     8761 cccacgtcgg gaggatctgg cttttaaagg tctccttttc ctttatgagc gcatccatgt
     8821 caaggccgat ggctttctgt gctccggctt tggatgatat atccggtacg atgactttat
     8881 tgacaccctt tgaagcaagg acctttgcca gttcaatcct ggcttccagt cctttcatga
     8941 taccgttgct gagtatcgag gccacctcaa tgggggcatg gaaggagccg ccgtgacttg
     9001 ctgcgcagaa gtcccatctc cactgcgatt gcctgatcag cgagagaacg ggtttcattg
     9061 cagcctcatc tgctcccgca tcccatgctg cctttgcctc ataatgggct gatacgagta
     9121 tctcctcaag gtagtttctc aggctggcta ttttatcctg cctttcatag acattgttca
     9181 tcagttcttc ctcgctctgc ctgtggcata cagcgcaggt tgacgagatc atgttgagcg
     9241 ggctctgcac gtgatggctg gtgtatttca caccgccctc ggcgacgaaa ggcatgtgac
     9301 agtctgcgca ggcgactccg cgctggccgt ggatgcccat tctgaacagt tcatagtcag
     9361 ggtgctgagc cttcagcatc ggggccttgc tgatggagtg ggtccagtca gcgaactggt
     9421 agctgtcata ataactttcg atttcgtcaa cggtcattcc cttgtcccag gggaaagtca
     9481 ggtattttcc ctcacccttg aagtaatatt ccacatggca ctgggcgcag acgaggctgc
     9541 gcatctcctg gtggcctgcc tcctcaatct ttttgccctg cctttcgaaa gcctccctga
     9601 gtgccgggcg ggttattgtc agggtcatgt tttccgggtt gtggcagtcg gcacaaccta
     9661 ttgagttcgt tatctccgga cccagagagg cccaggtggc cttgtagaag ctctctacgc
     9721 ccattttttc gatcattctg ggtacgtcag ggcttttgca tgcccagcat gttccgggct
     9781 gggggccggc atcaggagtc atgggtgccc cggtgcgcag tgtattacgt atatcctcca
     9841 cagcccagta gtggcccctt gcctgggaat agtcctttga gaatgcatac cctgcccaga
     9901 gcaccgcaag ctcggggtca gtggccagca catccagagg tttgctgccg gcatgcttgc
     9961 tccggaacga ggtgtcagag gtcatgagcc atgacttgta ttccctggga tagaacttgc
    10021 cccatacggt gttgtctgac tcgaaaggct gaaggtcagt gggcggcctg tatgcatatt
    10081 ttgcttccga ccgtcgttca acgatggagg aggccagcat gcccaggagg aacacgacaa
    10141 gagcggtggc gagaaacagg agccatcctg cccatggctt tttggatatg aattccgtga
    10201 ttgacatagg ttaggtatta ataattgtta caggttgtct ttcagggttg gtgttatggc
    10261 aaactgcgtt gatgagatgc tgttgaccct gtcatgcgga gtattgcggt gacagtcgag
    10321 acagtgcctg tcgcgtcttc cctccattgc ttcaggaggt cttccgggac cgtcactgaa
    10381 gagagactca tggcacctga ggcagttttc ctgaaccacg cgtttcccgg gttccctgat
    10441 ataaatggat tgcggttcgt tgcgcagggt aaagattgta gcatgccgga ggccgtcatt
    10501 tgccttgaaa tagtatttcc tgacgaagct gtcatgcggc acatggcatg cattgcagtc
    10561 ggtaatactc cggtgcgagc tgtgcgacca ggtggcgaac tgcggggtca tgatgtggca
    10621 gttgatgcac accctgggat catcagagag ataggaagtg gcattagata tcttcagaag
    10681 ataaaacgcc agacctgtga atgctccaag gagcattatg acaggcagtc gccactgtgg
    10741 cggaggcgtg aagaatctga tcagtttgtt tggtctgctc atggcagtgg cctttttatc
    10801 cggtcaatta tagatcgcaa cctcctcccc ggtatgccgg aaggatcaga tgaaattaac
    10861 gaagcagagc gccacaaggg cagtcccgta cctcttgtcg attgtcagag actccctgaa
    10921 gacgcttggt tctcccaggt aatactttga gaaggttctg tcatgcaggt catggagaac
    10981 agtgtagagt ctctcttcaa gagcctccat atcaaagtag ccggccagtt cacagacgag
    11041 gcctccgacc ttttcctcac aggtatcatc cttgtacatc catgcgaaaa tgactccggc
    11101 tgacacgtgt tcgccgtagt aaccgttggc tactgccatg atcgtacaga gttcggaacc
    11161 gtatgggggc atgtcaactt cgtcaagggt caccagcttt gcggttgcgg gcagcaccga
    11221 agaatagttc ataatgttgt actccgatat acctgcatca tgcagcgcca tgtgatatga
    11281 accgggatgg atggcatact cgctgcatcc tgagccctgt gttacgaaaa actcgttggg
    11341 aattctgttt tgggagactt taaccatgtt gcctttcaat actttagatt tatgccagtc
    11401 ataagaccgg tacaggcaaa tatacctaaa aataaaaaat caatgcaaaa gggatgttta
    11461 tatttcagat ccttgccata gcgaggacga gagagtatat aatcacagtt acggtgatgt
    11521 acattttcct ggcggtatca ggtttcactc ccgggaagcg gtatgttatc gagttgtgcc
    11581 tgcatgctgc aagacagtcg ccgcagagag tgcatgtaag gccgggcctg agcctggcta
    11641 tgtcgtccgg gctgagtgca tcataacggc agacggatga acagagctgg caggagatgc
    11701 aggtggattc aattctcatc ctgaacggat taatatgctt cacgtagcta acaagtgtgc
    11761 ccacgggaca ccagacagtg cagtgaatca tctttcctct ccgccttgaa attaagagaa
    11821 tgatgagtat ccctgtaact cccgtgccaa tgccggctgc cagcgcaaat ccctgtcctg
    11881 ctccgctgat tctcagcaac agtgcgactg caattgccag tatcaggaag ctgtttttca
    11941 gtgccagcct gtggcctgca ggccgggaag gtgccttttt ccctgcaacg gccgaatcaa
    12001 ttgctccgaa atagcagtac tggctgcacc atgccggacc tgagagaagg acagcggaaa
    12061 gaaaaataag tgtcattacg gaaagctctc ccctgtagag aggacctgaa attatgagag
    12121 ccgggacagg caggtgcaat tctccggtca tcaggaaaag cttgtctgca aagaggccca
    12181 gtgtcagctg gaggaagaag acagccgaga agagtgacca gctgaaccgt ctccaccatc
    12241 ctgatctgga tggcataagc atattatact ccatgactga agcaaagatt gcaatcccgg
    12301 ctatctgaag gtaaccgcca cccctgaaca acctctccag gagaagcatc ggcctttcgg
    12361 ccgcaaactg caatggcagc aggagaagca ccacaatcaa gccggtcagg gccggcaaca
    12421 gatacctttt ctgcatagtt ttctgactcc cgcaaaaata ttgaaaaaga ccaaagaggg
    12481 aagctgccga aaaaaatata aattggagaa cctaaaaaca ggttatgatc aggaggttga
    12541 tttcagtaat attttttctc ctccccgcgg tatatgacgc aacagctgag gttcaggaga
    12601 tagggacgcc cttcataagg aacttctcaa aggccgacta ccgggccagc acccagaact
    12661 gggctgtggc acaggatgca aggggattca tgtattttgc caataatgac gggctcctgg
    12721 tttatgacgg ggttcagtgg cacctttacc agatgcccaa ccactcaatg gtcaggtcaa
    12781 ttctgatggg ggcaaagggt gaagtattca ttggagccta taatgacctc ggaaaaatgg
    12841 tgcctgatgc cggcggcaat ctgtcattcc attccttcag ggaacagatt cctgaggaat
    12901 acaggaattt tgacgatgtt tggaacatct gcaccttccg cgacaaaaca gtattccagt
    12961 catattatgc agcctatttt taccgtgacg gaacaccaat gacagttgtg gcggcaccgt
    13021 cacggttcca gaattcatac agtgtcagag gccggctgct gttcaatgat accggaaacg
    13081 gacttatgga gttcgacgga acccggcttg tcagactccc cggatgcgag tccctgaccg
    13141 gaatggatat atggtcaata ctcccttacg gatccgggaa tgagattctg atctgcaccc
    13201 tgggaaacgg tttgtttatt catgacggag tgaagcttcg aaagtgggac gaaacactcg
    13261 gcaggattct gagccgtgac caggtgttca gcgctacaat gatatatgat gattattacg
    13321 tgatcggcac aatccttaac ggcgtgatga tcattgaccg tgagggaaaa attgttcagc
    13381 acatcaacag gaagaagggg ctgcaaaaca acacggtact tgacctgttc accgacagga
    13441 acggcaatct gtggctggcc cttgacaacg gcattgacta cgtgactgtt aattccccca
    13501 ttacctttat ccgcagttct gaaggctttg gcgccggcta tgcctcagtt atccatgagg
    13561 gtaagcttta cctcggaacc aaccagggac tatatgtaag aaactggtcg gagagggaaa
    13621 caggagacga tttcaggatg atacccggaa cctacgggca ggtgtggtac ctgggagtac
    13681 ataacggtga actgctgtgc gggcataata acggcaccta cctgatttca ggagaaaagg
    13741 ctgacctgat cagccgcatc cccggcggat ggaaatacat ggaactgaaa aaccacccag
    13801 gatacctgat aggaggcaca tacagcggaa tgatactttt ccggtgggag cagggcacgt
    13861 ggaaattcgt caggcagata ccgggtatga ccgagtcttt cagggttttc gaggaggaca
    13921 ggcaggggga gatatggatg agtcacggat tcaaggggat attcagggta aagctctcgg
    13981 ctgatctcga ttcggtgacc ggtgtcagat tctatgattc ttcagatggt ctgccctcgg
    14041 atttctatct tggcattttt cgtgtcctgg ggcagctcgt tgttgtgtca gagactgaga
    14101 tctatgaata ttcgcctgag gcagaccggt ttgtcaggtc aacctacttc aaccagcttt
    14161 tcaatcccct ggtggcagtc tcatatctcc gtgaagataa agagggtaac atatggtatg
    14221 tggcaaagaa cagggcaggg gtgttcaggc tgaaggagga tcattcatat gagcatgtcg
    14281 ccactccttt tcttctcctc tctggcaggt tcattcacgg gtttgagtct ttctacccat
    14341 attccccgga gcatgtgatc attggcacgg aagatgggtt tgcccactat tcatcgcgca
    14401 cctcttcact cgggtatgaa ggattttcgg tgtacataac cctcggggag gcgttgcatc
    14461 acgactcggt gttttattat ggccgtactc cctcgtctgc agatcaggag ataagctaca
    14521 ggttcagata tgctcacaac aacatccggt tcatgtttgc ttcgccggtc tatgacaatg
    14581 ccggaaatac cgaatacagt tacaggctgg gagggtatga tgacaggtgg tctgactgga
    14641 gccgtaccta ttataaggag tattccaatc ttcccgacgg aaagttcacc ttccatgtca
    14701 gagcgaggaa tcagctcgga atagagagca agcctgactc acttgacttt gtgatagagc
    14761 atccctggta taagtcgcct gtggcaattc ttgcctatgt cattgcaggg atgatccttg
    14821 tgctgctcct tgcccgtttt atcacctgga ggatagaact ctcaaggagg aatgagagga
    14881 tgaagaacag tcgtatctac gaggagaagg aacaggaata taaaaggcag gcgctggagt
    14941 ccgagaagga gattatcagg atcaggaatg aaaagcttca ggctgagatg atacttcgtg
    15001 acaaagaact tgctaaccag gccatgagcc ttgtgcgtaa aaatgaattt ctcaaccaga
    15061 tcaaggacga gctaaggaat gtgatcaatg gttgcaatga caggtctgcc ggcagcaagg
    15121 tttccgagat tctgcagcgt atagaccgag aggttgacaa caataagcag agagagatat
    15181 ttgaaagtgc ctttgatgag gtacacgagg agtttcttca gggtgtaaaa acaaaatatc
    15241 ccgagctgac tcctaccgaa ctgaggctgt gtgcctacct caaaatgaat ctcccaacca
    15301 aggagattgc ccctctgctt aatatatcag taagaggtgt tgagatctgc aggtacagga
    15361 tcaggaaaaa aatgggcatt accagggacg taaacctcac gacccatctt ctcagcatct
    15421 gaaaaaacaa ggttttgtag tatcacttcc ggcgattggt gtattttata cactcaataa
    15481 ctattttcta tatttcagaa aaacagcatt ataagttttt attgatgtat tattgatgta
    15541 tttaaaaatt atcactgatg tatttttgaa gtaaagcatg gcaggtgcta atcaatttca
    15601 ctttcttaac ttggtactct gcaatctgat ggttttcgga ccatctgata acctgataaa
    15661 ccaaactcta aaactcaaca agatttatga aacagacaat catggctctt tttttcattg
    15721 cctgttccct ggctttaaat gcgcagggac tgcagattaa aggagttgta acgtcagcag
    15781 acgacggaca gcctgttccg ggggtgagtg ttttcgtcaa gggaacaacc accggaacga
    15841 tgactgacat ggacgggtac tacagtctcg gcaatgtacc catgaactca actcttgtct
    15901 tctcctttgt ggggatgaag gcacaggaag ttgtggtgac tgagagcacc accattgaca
    15961 ttgctttggc ctccgatgtt gaaatgatgg acgaggttgt tgttgtggga tatggcaccc
    16021 agaagaagag ccttgttacc ggtgcaattg cgaaggtaga gggtgatgac ctgcgcaagg
    16081 cctctgacat gcgtgtcacc caggctcttc agggcaagac ggccggtgtg gtcatcacgg
    16141 ccaactccgg tcagccgggg tctcagatct cggtacgtat caggggggta ggcaccaacg
    16201 gtgatgccga gcctctttac atcattgacg gactgcccat gagcggatca gggacggatt
    16261 ttctgaacgc ggcagacatt gagtcaatag aagtgctcaa ggatgctgca tcggctgcca
    16321 tctacggtgc acgaggtgcc aacggggttg tactgataac cacaaaaacc ggccgcactg
    16381 acacaaagct gacagtgacc tatgacggtt attatggttt tcagaatccc tggaaaaaga
    16441 tgccgctgct gaattcacgt gaatacataa tgcttattaa tgaggcatca gtcaatgcag
    16501 gtctcgggcc ccgtttcacc caggcacaga tagagggttt tactgccgat actgactggc
    16561 aggatgagat gttttactac aatgccccaa aacagaacca ctccctttct ttcactggcg
    16621 gcagtgacag gctggaatat tcatcctcgc tgaattactt cagccaggaa ggcattgttt
    16681 ccaaaggcaa gtcaagtttt gagaaagtcg gctacagact gaatgcagct ggcaccttcg
    16741 gtctcttcaa actgggagga aatgtaaacc ttgcaggaat aaccagcaag ggtattgcaa
    16801 ccaacgacag gtacggtctt ggcctgaacc aggcccttaa catgccaccg gtggttccgg
    16861 ttatgttttc tgacggtaca tggggcactt ctgaggcata cggattcggt ctgcaggaga
    16921 tcaccaatcc tgtggccatg gcaagttaca ataactcgaa gacaagaacc tacaaactga
    16981 tcggcaacct gtacggcgag ttcgatttca gtaaggtatc cccggcgtta aagggactca
    17041 ctttcaggtc atcattcgga ggagaatatg caatagtcaa tactgacacc tatacaccgg
    17101 aatattacct cgatgccatg cacttcagca atattgacaa ggcttacaaa gggcttgaca
    17161 tgtggtcgag gtggaacttt gaaaacgtac ttacctatgc aaggacgttt gatgttcaca
    17221 acctgaccct catggcaggt acaaccgcgt tcaaggatat gcatgaaaat ctctggggct
    17281 cgaagaatga tctgatattc gatgattttg gacattcata ccttgacaac gcaactgacc
    17341 ccgagagtgc caacgcgggg ggaggttaca atgaacatac tgtctcctcg ctttttgccc
    17401 gtctgaacta tgacctgaag aaccgctaca tgcttactgc cacaatcagg agggacggat
    17461 cttcgaggtt cggaagcgaa aacaaatatg gttacttccc ttctgcctca attgggtggg
    17521 tcatctcacg ggaagacttc atgagcaatc ttaatgatat attcaatgtt ctgaaactca
    17581 gagcaagctg gggccagaac ggaagtgaga atataggcga tttcaattat acttccatta
    17641 tcggtaacgg cgccatatat tattttggtg acgggaagac acagtacaac ggtacggtgc
    17701 ccaccaagct ggccaatcct tccctgaaat gggaggcatc cgagcagacc aacatcggtg
    17761 ttgatatgac gacccttggc agcacactca ctgtcacact tgactactat ataaagaaga
    17821 ccaaggactg gcaggttgct gcacccattc caatacttgt aggcaacgga gctcccacaa
    17881 ttaacggagg cgaagtcagg aactcgggct tcgaagccca ggtctcctac cgtaagcaga
    17941 tcggccagct atacctcgga gcttccgtga acggtgcatt caacaagagt gaggtccttg
    18001 acctgcagaa tacggagaag gttctgaagg gcggtgatgg cggttttggc cagagcgaac
    18061 tgatatatgt aaaggtaggt ctgcctatgg gtgtcttctt cggtgttcag actgacggag
    18121 tcttccagaa ccagacagag atagactcct atacaaacag taccggagga aagattcagc
    18181 caaatgccaa gccgggtgac cttaagttta ttgatgaaaa tggcgatggt acaatcagcg
    18241 atggcgacag ggtccagatc ggctccccgt tgcctgattt caccggcggg cttaacctga
    18301 acgcagagat ctacggcttt gatttcaaca tgttcctcta tgcagccctg ggccaggagg
    18361 tatacagcgc aaccagacgg tatgacatga actttgccaa ctacacagct gaatggctgg
    18421 acaggtggac cggtgaaggc acatctgaca agtacccgag agtcactttt gctgacaaca
    18481 acctgaacgg aaagacagtc agtgatttct atcttcagga cggtagtttc gtcagactca
    18541 gaaacattac cctcgggtat tctctcccgc agaatatcac caaagctatc aagctcagca
    18601 agcttcgtct ctatgtatcg gctgagaacc tctatacatt tacgaggtat acaggctacg
    18661 atcccgagat agggggcggc gtattcgata acggaattga ccgtggcatt tatcctcagc
    18721 cgaggacaat catgaccggt atcaatgtta cattctgaca ccataaaaaa gaacagagtt
    18781 atgaaaaagt caattatata cagtcttttc tttctggctg tgatggtggc aggttcatca
    18841 tgttccaagg atttcctgga gctcgaacca aagaccggtc agacagaagc caactactac
    18901 aagacagagt ctgacgcctt cctggccatg acagctgttt atgatgccta tgcgcttcag
    18961 aactggcagt ttgccccgac aatggctgac atctggtcag atgatgcatt ctgcggcggt
    19021 tctgatgctt ctgacatgag ccagtggcag gatatggaga tgttcaagat ggaacctgag
    19081 aacaatacat ctgcggattt ctggagccgc tgctatgccg gaatctacag ggccaacctg
    19141 taccttcaga agcaggatgg cattgactgg aagacagagg gactcaagga gaggtatgaa
    19201 gctgaggtta aattcctgcg tgcctatctg tactgggatc tggtacgtca ttacgggtgg
    19261 gtgccggtga tgaccgaagt gcttccctcg gttgaggatt acaggaatct tacacaaagt
    19321 gctccttcag agatatttac gctcattgca tcggacctgc ttgatgccgt tgatgttctc
    19381 ccgaaccagg tcacacctgc agaaaagggc cgtgttacaa aaggtgccgt taaggctttg
    19441 atggcccgca tatacctcta tcacaccggt atgagcgcca tcagcggcct gggacttacg
    19501 gcagagcagt ggggtgacgg tactgcagtg atcaataagg cctatgttca gacggctctc
    19561 gaccaggtga taaccagcgg cgtctattcg ctgatacctg attatgctga cctgtttgac
    19621 tgggataacc agaacaatgc cgaggcggta cttgagatgc aatattcaga caaggccaag
    19681 tcaggcgact ggggcggctg gaacatcaat ggtaacttcg catcggtctg gttaagtgtt
    19741 cgtaatcccc agggtgacgc aaccgttttc cccggatggt cacttgcagt gcccacctgg
    19801 agcctggccg acgagtacga atcgggagat ccgaggaagt atgttaccct ctatgatgct
    19861 gaagagatgc tgactgatta caacagggca ttcatgaata ccggctattt caacaagaaa
    19921 tacatggcca gggctgatta ccagggatcg ggaggtgacc caagccataa cttcccacgc
    19981 aacttcatgg atataaggta tgccgacgta ctgctcatgg ctgccgaggt atggctcact
    20041 gacaacccgg gcaaggctgc tggttatctc agccaggtga ggaccagggc actcggtgcc
    20101 gcagctgaaa aaacctctat cacccttgac gacatttatc atgaaagacg cgtcgaattc
    20161 ggaggtgaag ggctgagaaa atgggatctg ctgaggagag gtcttgacta tgcaggacag
    20221 aagatcaacg ccagtttcaa catcccggcc ggtactccca acgctacgga cttcaatccc
    20281 agaaccttca agccggaatc atggggtatg ttcccgattc ccgccagtga gataaggaat
    20341 atgaacagcg gagtgctgaa acagatggtt cccgcctaca ttggcttatg aattatccaa
    20401 taaaaaatac tgtaatgaaa aaaatcagat atataagcag attagcgggc attctggccg
    20461 ttgtgctggc tgtcaatgcc tgcgaacctc agatggcgga caagcccgaa gtcggggctg
    20521 ccccttcatc cgatcagctc agcttcacta taactcccgg aagcgatgag ttcaacttcg
    20581 tgattgaaaa cacctcaaca gtcaccggga ttgcctcatg ggattttggt aacggcacca
    20641 aggccaccgg caactcaaat gtcgccaggt atccgatgcc aggcgattac actgtcgcga
    20701 tgacacttgt tacaagagga gggttggcta ccattacgaa gaccctgaca cagtccgaaa
    20761 cagacttttc catcttcact gatcctgtat atgtcaatct aacaggaggc atagatgatg
    20821 ctgacgggaa aacctgggtc cttgatgtgg ctactcccgg tcacctgggc gttggcccgc
    20881 ttgatgaccc cacagccatg acctggtggg cagcagctcc gtatgacaag gacggcacag
    20941 gcctgatgga cgatgagatc accttcaaac ttgacggttt tgctgttacc tatgacaata
    21001 agggtgtaag ctatgtcaag gcttaccgtg ccaatgaccc ggcactggca tccgtttatc
    21061 tcaatccccg cgaaaataag ggcgactggg acgtagacta cacaacaccg gtgtcaggca
    21121 ctttcaatat catcgaggta tccgaaggca actacaccat caccctgaat ggtgaaaaac
    21181 caattttccc ctgctttgac gttggcgcaa aggataatac ctacaccata cgcaaagttg
    21241 aggaaaacct cctcgaactc acctgcttcg aatcatacga gggttggaac aactggcact
    21301 attacctgat acccaagggt tatgtcaggc cgacaatcac ctatacagta aatgctactg
    21361 aggggactga caatgaggtt tcctgctctg tcacaggtta cagcatacct gcagggcaaa
    21421 gtgttacaaa tattgcctgg aactttggcg acggcagcga tgaagtcacc gccggcaagg
    21481 atgaagtggt gaaacatgtc tacatgcgca agggaacatt caccgtaact gcgaagctga
    21541 caaccagcat tggagtactg accggaacga aacagattgt gctggctaac aacaactctg
    21601 cctatgtgcc attcctcctt gacatgatga tagtctacaa tgacctgagc gaggtgcagg
    21661 tattccccct gcttgcggaa aactgcgccc tggatgtggt cgataatcct gaaaaggaat
    21721 atccgaacaa gtcctcgaag gttgccctct tttcgaggac cggcaaccag tatgccaatg
    21781 catatatcag gctgccggca ggattcaggt ttgacctgag gctgcagcag gtattcaagg
    21841 taatggtacg cggcaaggca ggagatgtta tcctgctcaa acttgagaat tcagacccgg
    21901 ccgaaccctg gaaaaccgga acggagaaaa cttatacgat ccggaaagat gatacctggg
    21961 aagttgcaga gtatgacttc aacggagtac ccggaggatt tgactggaca ggtgatatgt
    22021 atgccccgaa cgtggttact gacagcaggt tcaatcatga ttattacaac acattacgtc
    22081 tgatgtgcaa ttcaacggtt gccgacggca ctcacagttt ctacctggat gagctgtcag
    22141 gcccgcatgt tgagggtatc cataaatagt tgtttttcca tatcattgcc tgccctgaca
    22201 aggggcaggc aatgtttttc atatttttac aaacatttca gagaagacag atgaaactca
    22261 cgagactatc gctggttctg tcagcaggac tttttatgct gattaccagc tcatgtgaca
    22321 agggaaagag tgttactgac ggtgccgaag caaaagctga caggctggtt gcgcagatga
    22381 ccctggatga aaagatcggg cagatgtcgc agcttgacct tggttatttc atggaccctg
    22441 aacaattgaa acaggctgtc agggaaggaa gatgcggttc gctgctgaac ttccttggcg
    22501 ttgaacaggt gaatgagata cagcgcatag ctgttgagga gagcaggctc ggaattcctc
    22561 tgatcatcgg aagggatgtt attcacggtt accgcacggt tttccccatt ccactcggaa
    22621 tggcagcatc atggaaccct gaagctgtca ggaaggcatt ccgcatctct gccgtcgagg
    22681 cttcatccga tggcataaaa tggacctttg ccccgatgat cgacatcaca tgggatcccc
    22741 gctggggaag gatcgcagag ggatgcggcg aggatccgta tcttgcatcg acccttgcag
    22801 ctgcaatggt aaaagggatt cagggtgaca gcctcactga cccaacggca ctggcagcat
    22861 gtgcgaagca ttatgtaggt tacgggtttg ctgaagccgg acgtgattac aacaccacct
    22921 atatccccga accccttctc cggaatgtgg ttctcaaacc tttccatgcc gcgaaggaag
    22981 ccggtgcaca gaccttcatg tcagcattca atgacctgaa cggcatccct acatcaggta
    23041 accggtttac cctgaaaaag atacttaggg aagagtgggg ttttgacggt ttcgtcgtga
    23101 gtgactgggc atctgtcaca gaaatggtca accacggatt cgccgaggat gacaaacagg
    23161 ctgcagagat agcaataaat gccggtgttg aaatggaaat ggccaccacc tcatactacg
    23221 ccaatataaa ggctctgctc gatgaaaaga agataaccat ggagcagatt gacgatgctg
    23281 taagggatat cctcaaggtt aagtacaggc tgggactgtt tgacaacccg tatacagacc
    23341 ctgaagcagg gaagaggata cttgaccctt cattccttga tcatgcccgc actgtggctc
    23401 gccagagcgc tgttctgctt aagaacgagg atgccgcact acctctttct gaagataaga
    23461 tagcctcggt ggctgtgatc ggtccccttg ctgacgcccc gcgtgaccag ctagggacat
    23521 ggtgttttga cggcagggct gagaattccg tgactccgct gaaagcaatc agggatctgc
    23581 tcggtgacaa aaaagtcaat tatgtccagg cgctggcata cagccgtgat aaaagcaagg
    23641 caaacttttc cgcagccgtg gctgctgcgc gaaggtctga tgcagtgctc ttcttcggcg
    23701 gcgaggagtg gatcctctcc ggcgaaggtc agtcgagagg agagataaat atgcccggag
    23761 cgcagaccga gctgcttgaa gcactggctg caacaggcaa gcctgtcata cttgtaatca
    23821 tggcaggacg acccctggcc ataggcaggg aaattgaact ttccgatgcc gtgctttatg
    23881 cctggcatgc aggcaccatg gccggtccgg ctataactga cctgctcttc ggcaaggagt
    23941 ctccttcagg caagctgcct gtcacctttg tcaagggagc aggacagatt ccgttctatt
    24001 attacaggaa caacacagga agaccggcaa gcgatgagac atggacaccg tttgactcca
    24061 tccccacggt caacccgcag agctcactag gataccgtac tttccacctc gactacggct
    24121 acactccgtt gctgcctttc ggttatggtc tgagctacac gacttttaaa tacagtgatc
    24181 tgaccctttc atcgcaggtg atgaagattg acggaacact tactgtcagg gccagggtga
    24241 ccaataccgg gaacatggac gctgatgaga tagtacagtt ctacgttcgc gacagggtcg
    24301 gcagcataac acgcccgatc aaggaactga agggatataa tcgtctccgt atcagcaggg
    24361 gcgaaactac tgatgtcgag tttaccctga cggctgctga ccttgccttt ttcaacggca
    24421 ggggagaggt aaccgaaccg gggaagtttg atgtctggat cggaccaagt tcggaggaag
    24481 ggctccatgc cgactttatt ctggaataga ataatacaat ggcgaagatg atacaaatcg
    24541 gaaccctgtt cctggcggtc gccctcctgg cacccggatg tggccgctca gaaaaaccgg
    24601 aagagtttcc ggataagcca aacacaggag acaatgttct ggtgtggagc gatgagtttg
    24661 attacaacgg gttacctgat ccgaaaaaat ggggctatga tacggacggc aatgccacag
    24721 gctgggggaa caatgaatca cagtattaca cggcttccag gctgaagaac gccgaggtga
    24781 aggacggttt tctctccatt actgcacatc gtgaagattt tgaaggtttt aagtacacct
    24841 cagccaggct agtcacccgt cagaagggcg actggctata tggcagggtg gaggtaaagg
    24901 caaaaattcc tgacgggaga gggatgtggc cggcaatctg gatgctgccc accgactggt
    24961 cttatggcgg ctggccgtcc agcggtgaga tagacatcat ggagaatgtc ggttatgatc
    25021 cttacaagat cgttggcacg gttcacactg aggcatataa tcactctatc agcacacaga
    25081 ggggagcaac caccaatgtt cagtcctgct acagtgactt ccatgtatat gccatagaat
    25141 gggaggtcag ggagatccgg ttttatgttg acgacctgct ttactttact tttacgaatg
    25201 aaggcaccgg ctataagacc tggccttttg acaaacggtt tcatctgttg ctcaatgtag
    25261 cagtgggtgg caactggggg gggcagcagg gtattgatga tacagtcttt ccccggtcaa
    25321 tggttataga ttacgtaagg gtttaccaaa agaaaacagg gaaatgaaaa ggctctttgt
    25381 actgattatc tccatcctgc ttgtcagctg caacaggccg ccggaaaacg gtcctggcag
    25441 ggatgacgga accggaatta tggcggaggg gtggatcacc tcgccgcaga aaggtgtttt
    25501 attcacgaag cattccgggg agcttgagtt catgaccact gccaatactg atccggccat
    25561 cgttgttgac acaacgcagc gttaccagga gatagacggc tttggaaact gcctcaccgg
    25621 aggcagtgcc atccttctca ataaaatgag actggccgag cgcacggttc tgctgcgcga
    25681 gcttttcagt cctgatggaa acggcatcgg cctcagttac ctgagaataa cgattggggc
    25741 ctcggatctc agcgacaggg tctttaccta ctgtgatctg cctccgggcg aaacagatcc
    25801 tcagatggaa caattcagca ttgaaccgga aaaagctgat ctgattcctg tgctgaagga
    25861 gattctggcc atcaatcctt cgcttaagat tttgggctct ccctggacag ccccgctatg
    25921 gatgaagacc aacaatgcat cagtcggagg aagtctgaaa catgaatggt ttgatgccta
    25981 cgcaaagtat tttgtgaagt acatccaggc catgaaggac gaggggattg tcattgatgc
    26041 catcactgtc cagaatgagc ccctccatgg agggaacaat ccaagtatgg tcatgacggc
    26101 tgctgaccag gctctcttca tcaggcagag cctgggtccg gcatttgccg cggcggggat
    26161 aaagaccaaa atcattatct atgaccataa tgctgaccgg acagactatc cccttgccgt
    26221 gttgaatgat gccggggccc ggcagtatat tgacggctcg gcttttcacc tgtacggagg
    26281 ttcaattgac gccctcaccg cagttcatga tgctttcccg gcaaagaacc tttactttac
    26341 cgagcaatgg gtaggcggac ccggcaatct cgccgaagac ctcaaatggc atgttgaaaa
    26401 ccttataatc ggcgctaccc gtaactggtg cagaaacgtg atcgagtgga acctggcctc
    26461 tgatccatcg tatgacccgc atactgaagg aggttgtgac aggtgtcttg gtactgtgac
    26521 cctcagcggg aacaacgtca ccaggaatcc ggcatattat atccttgccc atgcctccag
    26581 gcatgtaagg ccgggatcgg taagggtatc taccaacacc cttggaaacc tgccgaatgt
    26641 tgcctttcag acccccgggg gaagcaaggt gcttattgtt gtgaacacca cacccgacaa
    26701 aagaagcttt aatgtaaagt tcaggggaaa cacacttccg ctcagccttg acggcggcgc
    26761 agtgggtaca tggatttggt aggctgttat tcctttacca ccatccgggg gaaactgccc
    26821 tcgcgggtga aaatccttat gatgtagatc cccggtgaca tggcgctcat gtcgactgtg
    26881 gtctcccggg tgccggcttc ctgcatcagc agtatcgttc ctcccatgct gtaaacgcag
    26941 atactgctta tttcaggttc tgcccttact gtaagccgat cacttaccgg attgggaaag
    27001 aggattgcag agatgtctgc cacagtttca ccggcagagg tgatcacatc agaagaagct
    27061 tcccagcttt ccggcaggct gttgtcaagc gcagtgtctt tcagacggag ataggcgccg
    27121 ttgccgtctg catcgggcca tggagtctcg tctgaataca ctgtaatgtc tatcacgttc
    27181 ccgaaggcat cggcgaggat gaggggctca ccgctgttgg acagatgcct ggtgaactgc
    27241 ccgaagggag agaagccgta ttttgcctgg aagatggtgg catttgatgc cagtatcacg
    27301 cacaggccgg gcccgagggt ggtattggca gggaactgat aaaccactcc ggtacccatg
    27361 aagtatatcc cgctcaggtt aacggtggag gtcccggtat ttcgtatctc catgaactcc
    27421 agatcatctc cgtcaggaaa ctgagtcgag gtctggggat ggtacataat cttcgtaacc
    27481 gtcagttgcg ggactgcaac gttgctgcat gtgctgtagc ttccaagccc gacggtcatc
    27541 cattcttcac gggcctgcat gaatgatttg atggcttcaa ttcgttccgc atgccttgcc
    27601 tgcttgcccc atctctggca gtcgcgtgca acggcctctg atatcagcgc cacagtctcg
    27661 tccagaaacg cctcccttac cgacctgttc aacggcatct ccggctgaag caggtcattc
    27721 cacctttttg agaggtaaca cctgaactgg ctgttataga acagatcgcg ccagaagcgg
    27781 gaaccattgt tgtaaaagta gtcttcaaag taccacaggt tggttttgct ccggtccata
    27841 tcccactgga acaggtcgtt gccaaaggag aggtcaaggt cccagacagg ccctgcgcga
    27901 agtttcccgt tgcggtcctt gtggaaatac gtgctgtact ggtaagcatc gggattggag
    27961 gcaaactcat ttacaatcat gaagtcaatg aaggaaggaa tgtctatcag tgtgggatat
    28021 ccgttcacca cggagacatc gttgttgttc gcggctgatt caagcctgaa gaatacattg
    28081 ctgatatatg atgtctgtgc agcggttgcg tcttcaggtt tgggcaggtc atggatatac
    28141 tccaccgggg aatgctggaa tgaatacatc gtccaggcaa ggggatcatc acctgatctc
    28201 ttgtcagcct ttgatatgta tccgccggat actgcgggaa gtgcgttgtc attcttgccg
    28261 atttttatca cgtcaatcct gtgatcgtcg gctttaagct tctcctggag catgtagagc
    28321 cccctgtagt tgttatttat gatcacctcg caatagactg ccctggaggc ataattgccc
    28381 atcttcccaa aaaggttatg gcagaagtag tcgcggatga aggcggtgtc aaacaccatt
    28441 ccgccgagta tccagtcatt ctcctcgggc atgccgagca ggctgacatt attgttggtg
    28501 gcatcatcag ccatgcgggt tgtgaaaccg tagttcttct tgggtgattc ctgtgaagat
    28561 gaacctctta tctcaatgcc tatcctgccg tcatagttca gatagagagg attatcctgg
    28621 tcggtcacat aattgcgctg tcccggtccc cggtcaatga tcttcattgt ggctttgacc
    28681 tttggctcgt tcgggatggt cacgctgccg tcagtgctga tgatgacaat gggcaggttg
    28741 ctgccggtca gcacctgtcc tgtcagtttc gcaggcagaa tcagaagcaa cagtgcatga
    28801 acagctatat atttgattat gcttcccctc acaggtgcca aaattagcat aattgtgctg
    28861 aaatagaacg tttcacgagc ccggtaaatt attgcgtcgg ataaacctgt aaggagcccc
    28921 cggggcatcg ttgatcaaga gtaaatgagg aaaatatcgg aacttaaata aatatatcgg
    28981 acaagggttt ctggcaaata aattatatat ttgcatcttt aatgctttaa gattggttgc
    29041 gtcccgatcc ataaaagttc tgaaaaggta catcatgtca ggcagaatgc agacagcgat
    29101 gttcaccgtt actacaacca ccatgaccac tcccggcaag ggagtaggta tattcatatg
    29161 atcccagggc ggacagcagt ccgcaaaccc ggctgagccg gagaaaagaa ttccattcca
    29221 ttacttaatc tgaacttttt tatgaaagtc ttgaaatttg gaggtacctc cgtgggaagt
    29281 gccggagcaa tacgcacggt agccgggata ctgcagggcg aggagggtca ggctacggct
    29341 gttttctcgg cattatccgg ggtaactgac aggctgacca gggctcttct tcttgcttcg
    29401 gaggggcgct tggagtatca ttccgtgatc agggaaattg agcggcgcca cctggatact
    29461 gcatctgagc ttctggaccc gcaacgttat gatggctttt ccggtatgat agccgttcat
    29521 ctttcccgcc tggacggtat actggagggt atttcaaggc tgggcgaagc aacatccagg
    29581 tcatcagacc tggtcttaag cttcggcgag ctgatgtcgc tagacctgat tgagctggtt
    29641 ctggaagagt acggtgcaga tgtaaggagg gttgatgcca gggagatgat ttttaccagg
    29701 cctgtcaatg gcagtgaacg ggtcgattac gaggcgaccg aaaaagccat aggaaaattg
    29761 cttccctgta ccaataagat cataatcacc tcaggattca tatccacctc cgtgaaaggt
    29821 tatccttcga cacttgggag ggggggatcg gattatacgg cagctataat cgcagcggct
    29881 gtcggggcgg agtcactcga aatctggact gatgtgagcg gaatttacac agctaacccg
    29941 gcaattgctc cggatgcctg ccccattgcc gggctgacat acgctgaggc gctggagata
    30001 agccatttcg gcgccagggt aatttatcct cccactattg agccggtgat gaaaagaaat
    30061 ataccggtct caatcaggaa tacctttgca tctgcggaga aggggactct catctcatca
    30121 gtgccaacgc caaatggcag tgcagtaaag gcagtcacct cggttggatc tgtctgcctt
    30181 gtctctctta ccggcagcgg aatggccgga gttgtgggta ttgcagcgag actctttacc
    30241 gtcctggcaa agagcaacat caacataata cttatcactc aggcctcatc agagcagtca
    30301 atctgcattg gcatagataa taaagacgga gagagagcct gtgaagctat agatgccggg
    30361 tttgaatcag agattgaagc cgggagggtg agacctgccc tgcgtgagga cggcttcgca
    30421 atagttgcac tggtaggaga gggaatgaaa cattctgtgg ggataagcgg gcaggccttt
    30481 gcagcactgg gcaggaacgg gataaatatt catgccatag cgcagggctc ttcggaactg
    30541 aatatctccg ttgtggtaaa ggaaacagat gctccaaagg ctgtcaatgc catccaccag
    30601 gagttctttc gctcagcgaa aagggtgatt aacatcttcg tggtcggaac aggaaacgtc
    30661 ggttctgccc tggtaaagca ggttatggat cgctcagcct ggtttgcggg agaataccag
    30721 accgagttcc ggatagtcgg tctggccaat accaggaaga tgataatcag ggaggaaggg
    30781 atcacgggtc ccgactggaa gtcggttatg atggccagtg accagaagag caaccttgag
    30841 agctttaccg ggcagatgtt ttcactcaat ctgcccaaca ctattttcat tgacaacact
    30901 tcaagcagcg atgtcagcgc cctttatgaa aggattcttg agaagagtat ctcaatcgtt
    30961 acctcgaaca agcttgctgc ctcatcgtca tttgaaaact acagcagact gaagagaacg
    31021 gcagtaagga agagggtgca attcgggttc gggagcaacg ttggggcggg gctgcctgtc
    31081 attcatacca ttcagaacat ggtaaagacc ggagacagaa tccaaagaat tgatgcagta
    31141 ctctcgggaa gcctcaacta catcttcaac tgctactcca atggaatgag attcagtgaa
    31201 gcggtcctgc aggcagtaga aaacgactat actgaacctg acccgctgac tgacctcagg
    31261 ggattggata ttgccaggaa gctgcttatc cttgtgcgtg aatcaggatt ttgtcttgag
    31321 gcttccgaga tagcgtttga agattttctg ccgggtcccg ttccggaaaa atatgatgct
    31381 gaaaggttct tcacacagat gagggagttt gatgactttt ttgaacaaca gagagaggaa
    31441 ctgtctctca ggaaggaaaa aataagaatc attgcgtgtt atgagtcggg aagggcatcg
    31501 atcaggccgg tgagggtcag tgcggagcat cccttttatt acctggaagg gaatgataac
    31561 attgtgtcag tgtattcagg gaggtattca ctcaggcccc ttataataaa aggggcagga
    31621 gcgggtgcag atgtcactgc agcgggtata tttgcagata tactatctat agtcaacagt
    31681 taacatggca ggaaagagga tcagggtaaa ggcgcctgca acggtatcaa atctcagttg
    31741 cgggtttgat gtgatagggc ttgccctgga tgagccgtat gacatggtcg atcttgaact
    31801 gactgacaac ggcacggttg tcattgatga tatcaggggt tgcggaacac tcagcaggga
    31861 tcctgaaaag aacgtggttg gtgctgtcct taaagcagcc tctgccatgg ccggcagcag
    31921 tgccggattc agaatcagca ttgacaaggg gatcagacct ggcagcggga tagggtcaag
    31981 cggcgccagc gctgcggcag cagccttcgg tgcaaacctc ctgctgggag agaaattcac
    32041 gccggctgaa atggtcaggc ttgcaatgga gggcgaaagg cttgcttccg gctcggctca
    32101 tgccgacaat gtagccccgg cattgctcgg aggaattaca ctggtacgca gttacgatcc
    32161 tctcgatatt gtcggactcc atgttccgcc agaactgtat tgtgtggtta tccatcctga
    32221 gacagagata aagacctcag ttgcaagggg attgcttgac aggcaggtac ccctcaccac
    32281 ggctgtgagg cagtggggca acctggccgg gctggtggcc gggctctaca gggaggatta
    32341 tgatctgata gggcgctctc tcatagattt tgtggcagaa cccaaacggt cagtacttat
    32401 caggggtttt gaccagctga agaagtctgc catggaatgc ggagctctgg gagccggcat
    32461 ctccggttcg gggccatcgg tatttgcact gtgcaggggc ggcgattccg cggcaacggt
    32521 gatgaagggc atgtcggaag caatgaagtc gcatgggatt ggatttgatg cttacgtgtc
    32581 acccgtaagc aggaggggag ctcaggtttg ttgatcggac agttgagaat ggcatgaaat
    32641 attataatct gaaagatcgg cgcgtaatca ccggcttcag ggaagcggta gaaagaggca
    32701 tctccccgga tatggggttg ttcatgcctg aacggatacc ccggttggat gatgctttct
    32761 tcagacagtt gccatcgatg acccttcctg agatcggcat tgaggtcgga tcactttacg
    32821 cgggagatga tataccgcga aaagtgatgc ggcagatatg cgaggaggct tttgacttcc
    32881 cggttgtcat caggcaggtg gatgaacgaa aacttgtcct ggaactgttt catgggccta
    32941 ctgccgcctt caaggatttc ggggcgaggt tcatggcagg ctgcttcagg tatttttcct
    33001 cccgtgatga caaacggagg gtggtgctgg tagccacttc gggcgacacc gggggagcga
    33061 tagcatcaag tttccttggt gtggaagggg tggacgtaat tattctttat ccctctggta
    33121 aggtgagcag cctgcaggag aatcagctta ccaccctcgg ccggaacatc acagccgttg
    33181 aggtggatgg ttcatttgat gattgccagg cgatggtaaa gagtgccctt gccggttctg
    33241 caaaccgcac aaatctaaaa ctcacctctg ccaattctat caacattgcc cggctgatac
    33301 ctcagagctt ttattattat tatgccatag gctgttcagg caacggaagg gtattgtctg
    33361 tcccaagcgg gaattttggc aacataacgg cagcagcaat ggcccggagg tcaggcctcg
    33421 gcattgacca cctggtggct gcaaccaacg tcaacagggt ggtgcctgat tatcttaaaa
    33481 cgggtattta cgctccggct gtatctgtcg ccaccatagc aaatgccatg gatgtaggca
    33541 atcccggcaa tttcgacagg ctcaggaggg tttttgatga cgactatgga gcaattacag
    33601 gttcggtgtc aggttactgg tttgatgatg accggataag ggaacatata ggtcaggagt
    33661 atcaacgcac aggctatctg cttgatcctc acggtgcagt tgcaagcctt ggccttgatg
    33721 cttatctgga acatcacccc gggtcaggag gtatcttcgt ggctactgcc catcctgcca
    33781 aattcaggga tatcgtggag ccgctcacag ggcagaccgt ggagatccct gacagtctga
    33841 gaaacttcct ggccgggaaa aagcagtcgg tgaaaatgaa agccggtccg caaaacatcg
    33901 aagagtacct tgatgacata tatcagaatg aataacaaga tcatgggggt ttcctgacat
    33961 gggttctgaa aaccgcgagt tccagatttt cgtcaagccg gttggcggca gatgcaacct
    34021 caggtgcagt tactgttact acaccggtca cagtcaccag gaaggaacag ccggcgggca
    34081 aaggatgagt gatgaagtgc tggaaacata tataaagcag catattcagg cggctggcgg
    34141 cggagaggtg tttttttcat ggcatggcgg cgagccggtg cttgcgggca ttgacttcta
    34201 caggcgtgct gttgcgcttg aaaagaggta taccccgccg gggtgcagaa tagttaacgg
    34261 catccagacc aatgcaaccc tgattgatga tgcatgggga aggttcctga aagaagaggg
    34321 tttttatgtg ggtgtcagcc ttgacggacc ggagtggttc catgacagca gcaggctcag
    34381 gactgacgga aggggtacat ttacctctgt gatgagagga ctggaaatcc tcaggcagca
    34441 tgatgtgccg cacgaggtgc tctgtgttgt cagcaactgt aacgtgcacg cttcacttga
    34501 gttatacagg tttttcaggg acctggggat tgagtttatt accttccttc ctctggtgga
    34561 gcgaaagagc cggaactcat cagaagtgac cgggagaagt gtcagcgcgg ctgacttcgg
    34621 gagatttctt gctgaggtct tcgacggatg gattgcaaat gatattggaa aaataaaggt
    34681 ccaggtcatt gaggaggcgc tgcgcacagc tttcggccag gatcacacac tttgcatctt
    34741 caatccggtc tgcggggcag taccggcggt ggagatgaat ggagattttt actcctgtga
    34801 tcatttcgtt gacggggatc acctgattgg caatataatg aaccagtcac tggagagcct
    34861 tcttgatgat cccagacaga aagcattcgg tgaagcaagg aaatcagcca tgcctcgcta
    34921 ttgtcttgaa tgtgaggttc ttgatatgtg caatggcgag tgcccgcgca acaggtttat
    34981 caataccccg gacggagaac cgggtctgaa ttacctgtgc gatggataca gatacttctt
    35041 caaccattgc cggccgttca tcaatgaagt ggcaaggctc tggaaggaag ggagttagct
    35101 tttcagtgtt tctttgaaga agtcggttgt ccgtttccag gccaggtcgg cagcctcctt
    35161 gttgtaccgt tctccggtgt cgttgttaaa ggcatgctgt gttccctcat agataaagat
    35221 ctggtattct atgccagcct ttttcagtgc ctcttcaaag gccggtatgc ctgcattgat
    35281 ccgttcgtca ttgccagcat agtgggccat catttttgcc ctgatctttg gcacgtcctc
    35341 cggtgcggga gttgatccgt agtaggggac agctgcatca aggtcaggtg agttgactgc
    35401 aacctggttg gtcattgctc cgccccagca gaagcctgtg cagcccacct tgcctgaagt
    35461 gcggggatgg gtttcgaggt acttcacggc tgccaccagg ttcctgattg tctcctcgcg
    35521 atcaagctgg ccgatcatct cgcgcccctt gtcctgatcc tctggtgttc cgcccagagg
    35581 cgagagggcg tcgggagcca gcgccacgaa tccttccata gccatccggt tgttaacctc
    35641 acggatatgc gggacgagac ccctgttctc gtggataacg atcacgccgg ggtatttgtc
    35701 ctttccttcc gggattgaaa tccatgcgcg catgtcaccg gaagcgcccg gataggtaat
    35761 aaactcgccc accactttca tggcgctttt accggcggtt cgggctgtgg ctccgttgca
    35821 gccgggcagg atcaccacgg ctgcagcggt tcctccggtc accagcgcca gtttcttcat
    35881 gaattcccgc cggcttacat taccgttgcg gtactcatca tacagttcgt ctactttcct
    35941 cttcattggt ttggtgcttt atttggggtt gaatctgtca gttcatcagg actttcgcgt
    36001 ctcttccttt attctctgga tatgtccgcg gccgaggcct ttcacctcgc agcccagctg
    36061 gaaataacgg cggatttttt cgtcagtcag gatgccgaag caatcaatga ttgctgccgg
    36121 ctggcctata gtcttcacca ccctgtcagg atcaaggctg aggtatactg aatgaggcac
    36181 tgccagaatc actgcctcag cgcctttgag agccttattc aggttctgtt caattcgtat
    36241 ctcttttagt ttttcctggt tgcgaaagaa ctgggccttg ctctgccctt cagcaggata
    36301 ggtgtcctgt tgttcgaact cccaccaatg ttcgacatac gggtcatgga cacgaatttc
    36361 agcacccatt tcggcgagtt tgcggatgac aatctcagat ccgctgtagc gggtatcacc
    36421 gacatcttcg cggtaagcgg cgcccagcac cagcacatcg gcggccgcaa tgggtttacc
    36481 catatttcgg agagcgtcgc gtgtcagctg ccccacgtgc agtcctcggg tgtcattgat
    36541 atcgatggcc atcggggtaa tcctgaagat gtcatcctcg aagccaagta tgtgcttgta
    36601 tgcccagacg ccgaggcctc cgtccttcgg gaggcagtat ccgccgatcc cgggtccagg
    36661 gaagatcatg ttgctgtgag tgggtctcat ttttatggct tttatgacct tgacgaggtc
    36721 gaccccgttt cgctctgcga agaggctcca ttcgtcgagg aaggcaagga tggtggcgcg
    36781 gtagctgttc tcgactattt ttgctgtttc agattcaatg ggcctgtcca tcaccgtgag
    36841 aggaaatttt tcagtgttga gcacttcggt caggaatttg acgacccgct tttttgcctc
    36901 ttcgttgatg ccgctgcaga cccgccagaa atcgcggatg ctggccacat aattgcggcc
    36961 cggcatcacc ctttcatagc tgtgcgccag gagcggatct gttttgattc cccgtttgct
    37021 gaaggctttc ttcatgatgg ggtatgccac ctgctcggtg gtgcccggag caacggtggt
    37081 ttctatcagc accagcgctt cggcggggat atgttcggca atgaccgcca gggaagcctc
    37141 gagtgctgcc atgtcagtct gtccggcacg aacgttgcca agttcttcct taatgtagtc
    37201 gcactgaacg tcaaccacca ccacatcggc cagcttgagt acgtcatagg tatatgtcgc
    37261 catgagcgtc ttcttctcct tcacgcaacg ggctatcatc ggatccacct cggggtcttc
    37321 ggccttcacg ggggagacgc cacggttgag cattggtatc ttccagaagc tgcgcacgct
    37381 cggacgttgc attcctataa caaacttgga gggttttcct ttcttgtcaa cggtgtcagc
    37441 aacgatggct gccataacag ccccgacgaa tcccaccccc atgaccacaa ctatctcgcg
    37501 gcctttggca cgttcctgtt ttacgcgttt cttaatgttc tcaaattcag ttttgtaatc
    37561 ttccgttttc ggaaggggaa atttctcgcc gctggggctg acagaatact tttcgctcat
    37621 attgttatct ttaaggtgta ttaatcggat gactaaaatt agtaaaaaga aacctgatgg
    37681 aaattctttc catgttaatt gaaacgcaaa aacacacctg agattatttc agaatggcac
    37741 tcctggttca tttccatatc tgcctggcta tttctgcctg catccatgat tccatatttt
    37801 cttctggctt tcatatcttt gtatctgacc gtcaggattg aactgatata ccgggcggtt
    37861 gttgtattga aggagaatga aaaggaaaag cccgatgaac aagatcatgt tgcttctgtt
    37921 gcctgccgtg ctgataatgg cagcttcctg cggaaaggaa aaacagtcgg agaggtttga
    37981 actcctgact gcacacgcgt ggaccagtga cagtctcctg gcaggtggtg ttgatgccag
    38041 cggaaccggt cagatgcttg aaaagttcag gggagaagtc accttcaggg aagacgggac
    38101 cggatcattt ggtcagtaca cggggacatg gatgttcgcc gacaatgaga caagcctgac
    38161 cattgcctct cccgaactgt cattcccttt gaccactcac attgtggaac tgaaggcaac
    38221 ttccctgaaa gtcaccttta catttcctgc agtcccacct gtcaatgtca gaatgacctt
    38281 caaacccaga tgactgcttt ccggaactgg cttgtttttg tattattcct ggctgccgcc
    38341 ggtgcggttg aaggccagac catagtcagc ggcagggtaa ctgacagtca gacaggtgaa
    38401 tccctggcag gtgtttatgt catctggggt aaaggctccg gaacaagcac tgataacgat
    38461 ggtcatttct catttaactc agatgagggc acccgtacca tagtcttcag atacattggc
    38521 tacaaatcac tgactgaggt acttgacatt actgcgggaa ataatcttgt tctggatatt
    38581 aagctggagc aggaggccca gtcacttgac caggtggttg taagcgccga caggatggag
    38641 cagaagcggt ctgacctcac tgtgtcaatg gatgtgatcc gttcagaacg gctgttccga
    38701 gctcatatca ccgatgcgca ggagctgatc accaaaactc cgggtatcga ggttcttgac
    38761 gggcaggctt cgatacgggg cggcagcggt ttcagctacg gagtcggaag cagggtgctg
    38821 gcgctgattg acgggctgcc gatgatgtcg gctgatgcag gtaatatcaa gtggcatttc
    38881 ctgccccttg aaaacctgtc gcaggttgag ataatcaagg gagcctcctc ggtgctgtac
    38941 ggctcttcgg cgctcaacgg agtcattaac ttcaggactg ctgatgcttc aaatgtgcct
    39001 gtcacacagt tctatgccga gacagggctc tttggtgccc cggccactga agaatggaag
    39061 tggtgggata gcccgaggct tttcggcagt acatcctttt cgcacctgcg aaagattaat
    39121 gacactgatc tgggtgtcgg ggtcaatatc tcctctgaca ggggttacag gaaatacaat
    39181 gatgagacca tggggagggt cagtgtcagg ctgaaacatt acagcagcag gactgagggg
    39241 ttgaaatacg gcataaacct gagcggtggc aagacctaca agactgattt tgtactatgg
    39301 gaaaacgcgc ttaccggcgg gctcatacag gatacggcaa ccgtatcgag gcttcacggg
    39361 tactacttcg ctgcggatcc ctatatctcc ctgggcaggc gcggtaaggt cagacatgac
    39421 atcaggctga ggtttcaggg ggccgggaac aggttccctg taaggagcga gaacaatgca
    39481 ggctcagttt caatatatgg cgagtaccag ttctggtacc gattctctga catggtaagt
    39541 gtcactgctg gtttttcaga aacctggagc gacatacggt ctgatttttt cggtgatcac
    39601 aatggtctga acctggcagg gttcgcccag gcggaagtgc atcctgtcag ccgccttaaa
    39661 gtaacgggag gtgtgcgtgt agaacagaat tatcttgaca gagaccctga caggcctgtg
    39721 ccggtattcc gtacaggact gaacttccag gctgcaaaat atacatttct cagagcctcg
    39781 ttcggccagg gataccgata cccgtcaatt gccgagaagc atgcatcgac caccctcggg
    39841 tccgtaagga ttttccccag tcctttcgtc cgggcggaat cgggctggag ctccgaggcc
    39901 ggggtgatgc agggtttcat gatcggcagg ttcagcggtg aaggtgacct ttcattattc
    39961 ctctcacaga gcagcgatat gatagagtat atcttcggtg tacatcagga ccctgaaagc
    40021 ggcgactctg accttggctt caaggctaca aacacagagt attcaaggat atacggcact
    40081 gagttggaaa tgctggtcag ccggccgttt ggaaaggtta cagccactgt aaccggtggc
    40141 tatacctata ttcacccggt tgagtttaat gccataacgg gacggagcac cggggtgtgg
    40201 ctcaagtatc gcagaaagca cactgcaaag attggttttc ttgctgtctc agaacgtttt
    40261 gaggcaggcc ttgatatata tgcgaggtca aagaccctga acatagataa tgtctttctc
    40321 agcccgttaa ccagggagga tatcctgccc ggttttttcg actactggca ggaacataac
    40381 cggggatatc tgctcatgga ctgcagcgtg ggctacagcc tcagcgaggc gttgaaactg
    40441 tcggtggctg taaagaacat taccaatgcg gaatacatgg ggcgtccagg cgacatacaa
    40501 cccatacgga acttcagcct caggctgggc ggcaggttct gaacattttc gctaaattta
    40561 cctcgtgccc cgtcttcgat tcatagctgc tgcagttatg cttttggtga tctcaccgat
    40621 ggcgacattg tcatacagcc aggagaatta tctgcaaatg acggatgata atgactgcac
    40681 caccgcattc actgactccc tggcaggaag gaaggctatc gattcatcag cttacgacat
    40741 ctggtcctgg agccttggaa cagacattgt gaccagttat atctggcggg gcgccaggca
    40801 gggcaggggg cctcatcttc agccgtacgc cgggttttcg gcaggtccgg ttacaggagg
    40861 ggtgtggggt acttttgatt ttaatggcta cagggaggtt gatctatacc tggcgatgga
    40921 actgccgggc ggattcatca tcggcctcca ggattactgg atggctgacc tgccattttc
    40981 tgattttacg gctgaaagcg gcagtcatgt tctggaggca tcattaggtt atgactcgga
    41041 acacatcagc ctgaatgcaa attacataat taatgaagcc ggaggggcgg gctcagttgg
    41101 agacgacctg tatgttgaga gccggttctc atttgaatac ttctctgttt tcatgggtgc
    41161 cgggaatggg tggcatactg aagatggccg gttcatggta tgcaacgttg gaatcggggc
    41221 ggaacgggag tttcaggtga gtgactcctt ctccattccg gtaacggcac agatatgcta
    41281 caatcctgac agtggaaggg cattcttcgc ggccggttta tccatttcca ccggcaggtg
    41341 accgttaccg ctgcccgcag ggacaggtct gttatccgcc gcagagctaa agaaatcaat
    41401 atttgcctcg ggctcaggtt ttcagaatct aatttctatc tttggaataa tgatatgtat
    41461 cagggatact gatactattc attactaacc tccgaagggt tttccgttta aacaaaatct
    41521 gattctgaag agaagggtga taatgtttga attttccgaa atacagaaca actacttctt
    41581 tttacccctg ataggttttg tgatcggaat ttttggaacc atcctggggg gaggaggcgg
    41641 attttttttc attcccattc ttacactctg gtttgccgta cctgctcaga ctgccgtgat
    41701 gacctcactg atggccacat tgcccataac actggttggt tctgtgagtc attaccgcag
    41761 gagaaacatt gatgtcagcg tagctgctgt tttcattgcc ggcggtctga tcggggctgt
    41821 ggccggtgcc aggattacag gctggattac cgagtcacaa cttaaggttg cctttggcat
    41881 ctattctatt atgatgggat ttggtattgc actgcgttca cggaggaata aagaggcggg
    41941 aaaactgaca gaaggccaga ataagaggga cggccgttcc atgattgcaa aaggatcatt
    42001 ctacggattg gttgcgggaa caataaccgg tgccttcggc acgagcggca caatgcctgt
    42061 aattgccggt ctctattcaa tgagaatacc gctgaaagtt attatcggaa cctccctgct
    42121 cgtggtcctt ttcaacagca tctttgcaat aagcgctcac ctttttatag gtaaggtaga
    42181 cgtcaccctt gtctctttcc ttaccgccgg ttcggtaatc ggtgcgttgg gaggcccggg
    42241 gatactgtca aggatgaaga ttgataataa ggcagagtcg gcagctgcat tctggtatgc
    42301 tgcaacgatg ataattattg gaattctgat gatccttggg gggaggaact gaccatgcat
    42361 ggaaccatga taaggacgat ttacataata atactgattt actttgcttt aggaggcgtg
    42421 ggtttctacc tgatcaacag gagaaagact ccggaagctg caagggagag ctatgttaaa
    42481 ttcggaactt attttctgat catcaatctg ttattcttca gtattatcct ccatacgatg
    42541 gccttcaggg tacttacggt cctgattata atcgctggat tcaccgaact gtccaccctg
    42601 ttcgtcaggt cggagcggaa taatgttccc ttttttgtct gttcattaat aatttacacc
    42661 gttttttcaa tcctcttctt catcttcgga gccggaacac gggaccagat tttatttgcc
    42721 ttcctggtac tttccatttt tgacagtttc agtcagataa ccggccagct gtggggtcat
    42781 cacaaactgg ttcccgcgat tagtccggga aaaacatggg aaggacttct gggtggtgta
    42841 ctgatatcac tattcagtgc ctggctgttg agtgaccttg caagggatac tatgcctgaa
    42901 atgattgtat tcacaatagg cattcctgcc tttgcttttt ttggcgatct gctttcttcg
    42961 gtttacaaac gtaagtataa agtcaaggat ttcaacaggt taatcccggg ccacggaggt
    43021 ttccttgaca ggtttgacag ccttatcgca ggaggtgcct gggtcgcact ctatacgatg
    43081 attatgttac actgagaata aacagatatg ggaaacacaa ttgtcttatc atttgtctac
    43141 ctggccggaa taagtatgtt gctgatcttt aatgaactgg tatacagaaa gtttagtcct
    43201 gaaggtgaga ttacccgaaa gtttgcgcat ttctcatcgg tgcttgcaac cctgccgttt
    43261 ccgtactttt tcccctcgca ctggtatatt ctggtactag cagtattgtt tgccatagtg
    43321 ctgttcagta cccggagagg aaaactgctt gggtcaattc acgcaattga gagaaaatca
    43381 atagggagct atctgttgcc aattgccatt tatgtcacct ttctgattgc ggagctgctc
    43441 ggtaacaagt ttcttttcat cctgcccatt ctgattctgg ctgtctgtga tccgctggca
    43501 gcaattctgg ggatgagtaa cacccacttt aacagaagga tcagaattgc aggaattacc
    43561 ctgaacaaaa cctggctggg aaccggttcg tttctggttt ccagtttcgt tatcagcatc
    43621 ctggctcttt atttttaccg ggaggtgttt gatttcaaga cattctggct tgccgcgctt
    43681 gtggctgttg tgacaatgtt tgccgaaatg ataagctggc ggggttctga taatctgagt
    43741 attccgttga gtgcagttct ggtgctggtt ttatgtcttt aattatgtct gatcatggac
    43801 actcacaatc ttcgtggtat cattggcctg aagggcattc aatgctgaaa atgagttaaa
    43861 taacataatg tcagatcaat aaagcaatat tatgaaacag ataatttata agggagagaa
    43921 tattctcaac gtcccgaacc tgataagcct gtaccggctg ctggcatttc ctgtaatttt
    43981 gtttatggca ttgaccggcc gtgaaaactg gtttgtcatc ctgatttgca tcagtctggt
    44041 cagtgatatt tttgacggca gtattgccag actatttaag ctgcagacca acttcggcgc
    44101 tgcgctcgat aacctggctg acctatgcac ctatgccatg gccctgctgg gcatgtttct
    44161 tttcagatgg tcagagataa ggccgcatgc atggatttta tacattttcc ttgcggtatt
    44221 cattttaagc taccttgttg cttttatccg tttccggaag attcccggtc tgcatctcta
    44281 ttcggcggtg tcatgcggtt atgcccaggg gatattcttc tttatcctgt ttgttttcgg
    44341 gttttatccc tggctgttct accttgtggt tgtttgggga ataattgcct atactgaaaa
    44401 gaccattgtc ctgctgaagc tcgatgatat taaaagaggg gtaaagggat tatactggct
    44461 tataaaaaag gaaaggatgc aggcaggctg agatgttgtg cggtccggtg aggcggatga
    44521 gagctttgcg gtccggtgag gcagatgaga gctttgcggt ccggtgaggc ggatgagagc
    44581 tttgcggtcc ggtgaggcgg atgagagctt tgcggtccgg tgaggcnnnn nnnnnnnnnn
    44641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    44701 nnnnnnnnnn nnnnnnnnnn nnnnnngatg agagctttgc ggtccggtga ggcggatgag
    44761 agctttgcgg tccggtgagg cggatgagag ctgtgcggaa ccagcgggat agctgacaca
    44821 tttacacgca taaaaaaaaa gccacggatt tcgtggcttt tttctgtgca aggtcaggtc
    44881 ctttatttgg ctgtaaccct gatgtcaatc cttctgttca tagccctgca ttcaggtgtg
    44941 tcatttgcag ggcatacagg atgctgtgag ccatagcctt cagcctcgag acggtttagg
    45001 gcaacgcctc tctttaccag ttcggctttg gcagtctcag ctctctcgga tgagagggca
    45061 ttgtttacca catcagaacc cgtattgtcg gtgtagccgc caatcttcag ggtcactgcc
    45121 gggtatgcct tcataattgc agctattctg tcaagctgtg aattcgattc ggctttgagg
    45181 gtactgcttc cggtctcgaa atagagcctg tcaagggtga accatgtagt cttgtcgatc
    45241 ggcagattgg catcttcgat gaactcgagg attttgtact caactgagtt tacgccaatt
    45301 tccatttcag tgccatccac cagtttcttc ttaatgctgg cgcctaggtc ataaacccag
    45361 tcacctgcgt cattaagcgc tcccttgatt gatgctttga tgtcagatgc cgcctggtca
    45421 aggtcattga ctatctcggt agcctggtct tccacctttt cctttgtgcg gttgcatcct
    45481 ctgccgaaca gcagccacag aaggatcagt gcacccaaca ggagaatgag ccatgaccag
    45541 ttgaattttg gtttttgggt agcagctccc gccacttttc ttgctgtgtc atcaacggca
    45601 tctcttgcct tgtcggcaaa gccgagcaga ccgccgccga tacctgacgg cagagcgcca
    45661 aggatggctg atttgtcagc gagcagcttg ccgatgatgc cggtaaatga aagacctttt
    45721 ctcttagccc agcggctcag gaatccgatc accatcgggg caacagtggt cagcagtttc
    45781 ccgcttttat cctttgccag gccggttgcg ccggacagag aaccgaggat ggagtcgaga
    45841 ttgcctccga acaggctgcc caacagtcct ttgccggcat cggcaaattt cgtgtcaccc
    45901 agcatgccga gccgcaggtc atcgtcatcg tcgccatcaa acagatcagc aacggtattc
    45961 caccatgaag gggcggaagt ggcgtttgcg cttttgccca gtatgccacc caggactgtc
    46021 gggatcatag cggtcagtcc tgattttgtt cttgaagcat cttcacccag attacggctg
    46081 atcgcatcga tagccttgtc tgtgaatgcg tcttttaatg attgaaaaag actttctgac
    46141 attgtaagtt gttattagtt agt
//
LOCUS       DAOPXU010000008        41442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_814_length_41442_cov_11.943678, whole genome shotgun sequence.
ACCESSION   DAOPXU010000008 DAOPXU010000000
VERSION     DAOPXU010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 41442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41442
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_814_length_41442_cov_11.943678"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PKK03_01530"
     CDS             <1..638
                     /locus_tag="PKK03_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83101.1"
                     /translation="DSSFSTAFDTTCTLEFIADIDLFVDAGMALKDSLSRATSVRVNT
                     LTVELYSVPWDEFEYHGCFDCDWDEIDYQGLSEDPPQLFPFVIGQKNYNATSKCFILY
                     AKTFWYLSPADVSGFIDDEPTANTLTFEQIEKGDFTWEAAQLSALESVMVMLPAAESW
                     DSLAVSENRAIFQYTVDDLFDGESLEQIWLRADFPIDENVSLRTGKFTPGK"
     gene            complement(669..1058)
                     /locus_tag="PKK03_01535"
     CDS             complement(669..1058)
                     /locus_tag="PKK03_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83102.1"
                     /translation="MKKIILLLLLVLTGVGAFSQTYKSRAATIRRDGFEIDRTERSIV
                     ISDREIVISNYLNGNTEPLRLEVYLTEDKEDRFDGICRHYYCKAKNDKTLSPCKKIVV
                     IRKPYSITLELYLSDDNKYVHDLEING"
     gene            1242..2186
                     /locus_tag="PKK03_01540"
     CDS             1242..2186
                     /locus_tag="PKK03_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNX83103.1"
                     /translation="MKTFVLAAICAVIIFSMPITVSAQSGVSPKKVLEALAGREFKHA
                     LDLAGKVDEPNYSDKSGVTLLMYAAETGAADVCRVLLERGADHSLRTANGTTALYLAA
                     QNGHTGVVRTLLEKGADPDIRPDNGATPLIAASEYGHTDVAALLVEKGADASLQDNHG
                     STALILASQNGNEEIVRILLGKGIDPDIQDLNGSTALILAAQEGQTEIARMLLQSGAD
                     VNRQTLNGNSALILASLKGNMETVQMLLDSGAPVDTKTTTGATALFAAASRGHCQIVS
                     FLLEKGADPMIRDTKDKTAYMYAKDYNVKSVLTSWSKK"
     gene            complement(2191..3672)
                     /locus_tag="PKK03_01545"
     CDS             complement(2191..3672)
                     /locus_tag="PKK03_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HNX83104.1"
                     /translation="MKRLIILLLITVFVVACHRGGQQHDSTPEAPDTGDLTMDAGLQK
                     VFSMRREAILDTIGDGLLILRSDYGFDGGRHEYRAAGNFWYLTGYGQPGALMSLSGTD
                     ARPYTLYTRERSIREIIYSGNLPDPGELLITWAPDTVLDFREADRNIAEAAREGRHIY
                     LDFRDRVFRDHVQEILGTENSAGALLRDINPALSEMRVDKDEYEVMMLQKAVDITGKG
                     LEAVLAECAPGMFEFEMEALLEYEWRRNGSNMPGFESIVGSGENAVTLHYSANNRKME
                     EGDLLLMDVGAEYGYYTADITRTVPVNGRFTEEQRDIYDLVLRAQKAAIGEMKPGAPM
                     TAAHRMATNTIIDGLRSLGLVTDPDCQWQRKFYTVYHICHFLGLDVHDTGTQGIPRSM
                     MSSYLIADTAVGRPLEAGMVLTVEPGIYLRANGLDQLELLYGSEAGEGEIADFISKVR
                     PVYEKYSNIGVRIEDDVLITGTGNTVLSASIPKEPDEIERLMK"
     gene            3777..4658
                     /locus_tag="PKK03_01550"
     CDS             3777..4658
                     /locus_tag="PKK03_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HNX83105.1"
                     /translation="MTMNRTAILFTLVSILILSFRSDKPAYNLYDSEGKNIKYEKMLD
                     RISSADVVFFGELHDNPIAHWLEYEVTADLHKIAGERLVLGAEMFEADNQLLLDEYLG
                     SEYEADKFEAEVKLWKNYRTDYRPLVEFAKKSSLPFIATNVPRRYASMVSRGGFEELD
                     SLSAEAKKYLAPLPVSYDPELKCYKDMLSMHGMPGMGARPNQNLPKAQAIKDATMAHF
                     IAENLPSGKIFIHYNGAYHSDNYLGIVHYLKLYRPGIKIATITTVLQDDITDLEVENR
                     GLADFIIAIPLTMTRTY"
     gene            4772..5506
                     /gene="ric"
                     /locus_tag="PKK03_01555"
     CDS             4772..5506
                     /gene="ric"
                     /locus_tag="PKK03_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HNX83106.1"
                     /translation="MDITAKSNVGDVVRFNFRTSSVFQANNIDYCCGGNKTIDEACKE
                     SGADSDRLIAELRSTAESTDPDTEYINSLSLSELSDYIIRRHHSFVRKNIPFLKEGLD
                     RICEVHGNAHPELFRIRELFNESSGDLTIHMQKEEIMLFPYIQQMEASARGGSPLGKP
                     HFGSVANPVRMMISEHETEGRRFDTIAEISGNYSLPGDACNTYRATLSGLKDFETDLH
                     RHIHLENNILFPKAVEMESGLIGKQS"
     gene            5614..6237
                     /locus_tag="PKK03_01560"
     CDS             5614..6237
                     /locus_tag="PKK03_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNX83107.1"
                     /translation="MQTRVREILDQEAEAVRKIPVTESYSQAIELMHKHVHLLKGKLV
                     TSGMGKAGQIAHDIATTFSSTGTPAVFLHPSEAQHGDLGVLQENDLMLVISNSGRTRE
                     ILELLELKDNLFPNMPVVVISGNNASPLWERASVGLFTGGPAEVCPLGLTPTTSTTVM
                     TVIGDVLVALMIEKIGFTAAEYAKRHHGGYLGYKSRERSGYSGGTIY"
     gene            complement(6231..7022)
                     /locus_tag="PKK03_01565"
     CDS             complement(6231..7022)
                     /locus_tag="PKK03_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HNX83108.1"
                     /translation="MKNSEIIERIKSKKAFISDMDGVIYHGNKLLPGVPEFVEWLKKE
                     GKKFLFLTNSSERTPKELQEKMLRLGIDLDENVFYTSALATAQFLATQKPGGTAYIVG
                     EAGLINAMYNAGYTMNNYNPDYVVVGDTRSYSFEKIEQAVNLVLKGAKLIGTNPDLTG
                     PVENGIIPATKALISPIELSTGRQAYFVGKPNPLMMRIALKKIGCSNEETIIIGDRMD
                     TDIIAGIESEIDTLLVLTGISTLKTADKFAYRPSYILEGVADLVQ"
     gene            7285..8628
                     /locus_tag="PKK03_01570"
     CDS             7285..8628
                     /locus_tag="PKK03_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HNX83109.1"
                     /translation="MTTGATPAPMSRKNYIVGMTVIAIFFFIFGFITWLNGILIPFLR
                     TACELTDFQAYFVTTAFYISYFVMSLPSSAVLKKTGFKNGMSIGLWIMAVGALIFIPA
                     ALARNYAIFLLGLFVQGTGMALLQTASNPYVTIIGPRDSAAKRISMMGIANKFAGAIA
                     PIILGAHIMKDSHVLDRQLAEATDAVMRAGILDQLATRVINPYIIMAVILVALGLLLR
                     FAHLPEVDTDAEESSVGETNVNKSSIWHFPHMLVGAVALFFYVGVEVIAGDTIIRYGQ
                     SLQIDMSSAKYFTSLTMLGMIIGYICGIIFIPRMLSQVNALRISAILGIIFTFGAVLS
                     PAGQQITMPFRDLVTFQAVTLTIPVTVLFVALLGLANALIWPSMWPLALNGVGRFTKT
                     ASALLVMAVVGGAIIPLIYGKLADKFSTQAAYWVCIPCYLILLYYSLHGYRLKGR"
     gene            8638..9738
                     /locus_tag="PKK03_01575"
     CDS             8638..9738
                     /locus_tag="PKK03_01575"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNX83110.1"
                     /translation="METDLKSIFSRFRGEGTFLEGKSFGPGHIHDTFRIITAEPECDN
                     YIIQRLNSNVFRDIPNLQENMERVTGHLRNKIAAMPGSDPKRECLTLVPDRETGRSFI
                     TDADGRYWRMFVFIEDHRSYEKVDSAHKAFEGGRIIGRFQAMLADLPGEPLHETIPDF
                     HNIARRLDAFHRVISRDPAGRVAAVRSEIEEITSREEEMKTIIRLGAAGKIPLRITHN
                     DTKFNNILFDKNDRALLIIDLDTVMPGYIHYDFGDAIRTAANRTEEDAEDLSAVSMDI
                     NIYQAYAEGFLSETRNTLNATEIEYLPFAPKLLTYIMATRFLTDFIDGDNYYKTRHPL
                     HNLQRVRTQITLLRDMEARYDNMVQIIKNITK"
     gene            9735..10415
                     /locus_tag="PKK03_01580"
     CDS             9735..10415
                     /locus_tag="PKK03_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNX83111.1"
                     /translation="MKKIILLFIAVFGLTLSASSQAETAVTDSSKFAAYWWHSKDMYD
                     HLPDIRNEIVFLGNSITDGAEWFELLDNKKCLNRGISADITEGILLRLDAITKLQPKK
                     LFIMIGVNDLSRGMTPDEIIVNYRKILDRVRTESPRTKVYVQSVLPVNPATGQAKNHT
                     NKTPQIMELNGKLKELAAGFGHTYIDLFSLMADPENHLPRSFSVDGLHLTYKAYKTWA
                     DAIKPYVK"
     gene            10570..12126
                     /locus_tag="PKK03_01585"
     CDS             10570..12126
                     /locus_tag="PKK03_01585"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018107237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNX83112.1"
                     /translation="MEINGKYFRSFIEREEALKKQYSAERIKKQHSKGKLTAHERIAL
                     LFDDGTFEEIDAYVTPSDQGVEFGRMELAYGDGVVTGHGKIDGRLVFAFAQDFTIMGG
                     SLGIVHARKIAKVQEMALKMGAPFIGINDSGGARIQEGIASLNGYGVIFNNIIQSSGI
                     IPQISVIMGPAAGGAVYAPALTDWVFMTSNTSYMFVTGPNVVREVLNEEITSEELGGA
                     EVHARKSGVANMIYDDEENTIIAVRKFLSYLPSNNLENPPVATYADETPGINPKLRDI
                     VPDDPNKAYDVRDVINQIVDRNSFFETSELFAGSLVTGFGRLEGKTIGIVANQPKVLA
                     GVLDIDSSVKGARFIRFCDAFNIPILTLEDVPGFLPGKDQEHSGIIRHGAKMLFAYAE
                     ATVPRITVILRKAYGGAYIVMNSKGMGGDFNYAWPSAEIAVMGPDGAVAILYRRELAE
                     AKDPVALKKELVKKYREKVANPFIADEKGFIDEVIDPALTREKVLSAFSALSNKSVKV
                     PSRKHGNMPL"
     gene            12222..13730
                     /locus_tag="PKK03_01590"
     CDS             12222..13730
                     /locus_tag="PKK03_01590"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNX83113.1"
                     /translation="MKKITRILIANRGEIAVRIMRTARKMGIETVAVRTAVEPDAMYL
                     SHADFIHEEDDELAEIPVFLDVEKLIAIALKTKCNAVHPGYGFLAENAYFAERCRDNK
                     ITFIGPSPDAIYKMGNKPVARQIAMKHKIPMAMGSTGSVANEDEAVRHAARIGYPVII
                     KAASGGGGRGMRIVRMESEMEKMFILASREAEKAFNDPSVFIEKYIENPKHIEFQILG
                     DTHGNIIHLGERECSIQRKHQKLLEEAPSPALDEELRAQMGETAVRIARAVGYYSAGT
                     VEFLLDSDRNFYFMEMNTRIQVEHPVTEMITGIDLIEQQIRIASGEELTLRQKDVKLA
                     GWAIECRINAEDVQAGFAPAPGKIEKLSLPEGPGVRIDTGVRAGSSIVASYDSMIAKL
                     IVTGRDRHEAIINMKQALDKIWIKGTKTTLPFFRMLMRHQAFQAGTFTTSFIEKDLDR
                     YHFSSEYEETIAAWIATKLFTEENLSSGKPGISFDQGRELTPWLLRKRLTQF"
     gene            13792..14322
                     /locus_tag="PKK03_01595"
     CDS             13792..14322
                     /locus_tag="PKK03_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNX83114.1"
                     /translation="MTPEKPAIKKLYALSNEGRKFAFSLPLKNVVRVNGRDYSVELRE
                     DEKYGVHVLWKNRRYPVEIVRTKQNRYEILFNDISHTFTVETPVSMQRKKALGTAREK
                     STTLIVRAPMPGRIIDVLVREGAEVVRGEALVILEAMKMQNEIQSVVSGTVVRIGVRS
                     GQNVMKDDILAEIKVR"
     gene            complement(14352..15809)
                     /locus_tag="PKK03_01600"
     CDS             complement(14352..15809)
                     /locus_tag="PKK03_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HNX83115.1"
                     /translation="MSEIKNLEPKLLWKYFYEITQIPRPSKKEHRMTEFMKKFGQEHG
                     LETIVDKTGNVVFRKPATKGMENRKGIVLQAHIDMVPQKNADKKFDFEKDPIETWIDK
                     GWVKAKGTTLGADNGMGVAAAMAVMTDPKLTHGPLEALFTVDEETGMTGAKNLKKGML
                     KGEILLNMDSEDEGELYVGCAGGVDVGATRSYKQEKTPQGMTAYKLSLKGLRGGHSGL
                     DINLGKANANKLMFRFFQQAECDFGMRIAEYSGGDLRNAIPRESFATIVVPSIKKAQF
                     EKFMKGYEKMYVAEFKEVDPDIKLIFKVADEPAKVMNEADQYRIIRGVFVCPNGVQRM
                     SASMPGVVETSNNLSIVRIGNGKFEAFCLCRSSVESAKEATAWKIAAGFQLAGCEVEL
                     SGSYPGWKPDMNSAILSAMKDTYNKLYGRVPEIKAIHAGLECGIIMGVYPKLDSISFG
                     PTIRHPHSPDEKVEIESVGKFWNFLVATLKNAPKA"
     gene            complement(15828..17210)
                     /locus_tag="PKK03_01605"
     CDS             complement(15828..17210)
                     /locus_tag="PKK03_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX83116.1"
                     /translation="MEKIRKTLAESKAARWTALAVVAFTMLCGYYLTDVMAPLKTMLE
                     QQLSWSSSEYGTFTGAYGWFNVFLLMLILGGIILDKMGVRFTGKMATLLMVIGTAIKY
                     WAVSTNMLDGQILFGMKTQVLVASLGFALFGVGVETAGITVSKIIVKWFRGKELALAM
                     GLEMATARLGTMLALQTSAPIAKALGHVSMPILMALILLCIGMIAFFIYCGMDKKLDA
                     SIAEADKGMVKEAEESFQLKDIGFILRNRGWWFIAILCVLFYSAVFPFLKYASDLMVN
                     KFGVDPETAGIIPSLLPLGTMVLTPFFGNLYDRKGKGATIMIIGAVLLIFVHAMFSIP
                     MFNQPWQAVLLIIILGIGFSLVPSAMWPSVPKIIPMKQLGTAYALIFWVQNWGLMGVP
                     MLIGWVLDKYCITGSRMIDGISVPTYNYQVPMMIFTSLGILAILFGFLLKAEDRKKGY
                     GLEMPNIVKE"
     gene            17391..17912
                     /locus_tag="PKK03_01610"
     CDS             17391..17912
                     /locus_tag="PKK03_01610"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HNX83117.1"
                     /translation="MKKLIFVMLLASAGLAVSAQEPAVKWYSIEEAVALAKTSPRPIF
                     VDAYTDWCGWCKKLDKDTFSNPVIAEILNNKYYAVKFDAEGKEPVTFQGRKFVNDGSL
                     GKTHQLAYALLQGKLGYPTVVFLTASSELITPVSGYKTPAQIEPMLSYFAGTSWQKQS
                     YDDFLKTFQGRVQ"
     gene            17983..18537
                     /locus_tag="PKK03_01615"
     CDS             17983..18537
                     /locus_tag="PKK03_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83118.1"
                     /translation="MIPELAQSPYQITPQLEDAMSTTVKYLGRKVKPSSSMLDVGQKN
                     PLTELLEKGDMKLKITNTEGDLDVFTLHDTNIYDYILYSHTIEHQFNPLHTLLELKKV
                     MSDKTLMFIILPRRGKLLWCKGHFHEIDDYRMKLLLERAGLDVVSLELHKAWRPWYFY
                     LTGFKPIMRLFLEYNAYYEVRLHR"
     gene            18604..19674
                     /locus_tag="PKK03_01620"
     CDS             18604..19674
                     /locus_tag="PKK03_01620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX83119.1"
                     /translation="MGNKILYIFPESSTFIRKDIGFLSIHYRVITPEHDWKIKSQVPL
                     NFIRQFFFLCRNLRGSGAVFVMFGGYWSFLPALTGKITGIPVYIIPGGTDCVSFPSLN
                     YGSLRKPVMRTFIRWSFQLCTKLLPVDESLVLSGYTYLPDADYPKQGFMYFFPRLRTP
                     YRVVHNGFDPDFFSTDPAAKRADSFIAVAPVSNMTRVRVKGVDLVIHLARRFTGCTFT
                     LVGVADDVIRALSPLPSNLVVYPWLPKEKFIGLLAGSRFVLQLSVSEGFPNALCEAML
                     CHCIPVGSAVGAIPSIIGDTGFVIASPERKYLEKRFEVILGTESGEQNRLGIMARNRI
                     AEMYHISRREKAFLEILDEIPG"
     gene            complement(19697..20818)
                     /locus_tag="PKK03_01625"
     CDS             complement(19697..20818)
                     /locus_tag="PKK03_01625"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HNX83120.1"
                     /translation="MKKQTVILLAALTLTIAGCSVFSGKSPGDTVAGPLGNKTTVTDG
                     CLVYALPMTVFELDILAEKRTEVPGPYAGYASELIGLDNVITVQTEKWTLAGVRLSAV
                     EELDPSQFYIIQGTALMQTNMLALRKSGLVLDINPDVYSRTTHSEQHGGPDYAGVLFP
                     DRGAYEYVATKTDTAYRLVKVDTAFIRVPYLVQKKKGMSVAEEAREAADRLLELREGR
                     HMILTGETNVFPQDGAALDEINRLEREYMALFAGKSLTETRHFRIWVTPDPEMAGRKT
                     TIFTFSETAGVNTSADGPGEPVLMEITPSGKTRELNMVVRPASPQKQANAADMLYYRV
                     PDVAEINVSLAGENLCTARRLIYQYGSLVTLPANYILGR"
     gene            complement(20827..22356)
                     /locus_tag="PKK03_01630"
     CDS             complement(20827..22356)
                     /locus_tag="PKK03_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNX83121.1"
                     /translation="MKIFTSAEIREIDAATVRLEPVTPAGLMERAATTLFQQVKDIVA
                     PERKINVFAGPGNNGGDGLVLARLMHEDGYAVRVFVVETGAARSSEFSLNAERLERSG
                     VIPLTIADPGQVPAIGRDEVIIDAIFGTGLRKSPSGVAAGVIRRINETGAFVISVDVP
                     SGLFCEDNQASDRDSIVRASKTLTFQFPKLSFMFAGNSDYTGEWEVTDIGLHADTISS
                     MNTPYHYVLRETVRPMIKERNKFDHKGVFGHVLLIAGSYGKAGAATLSAGAALRTGAG
                     LVTVHTPSSAVLALQAALPEAMVSPDSGTGNVTVLPDLGPYDAIGAGPGLGTDPDTVS
                     VLKDLLKNISVPLVLDADALNILAQNREWLDLLPPETVLTPHPGEFRRLTGTESTDFR
                     LMQEQKDFAVRHRCVVVLKGAHTSVALPDGRVFFNSTGNPGMATGGSGDVLTGMITSL
                     LAQGYDPSGAAVTGVYLHGLAGDIALRIHSEESVIAGDILMNIGRAFRETNFNNFNVY
                     L"
     gene            22506..24305
                     /locus_tag="PKK03_01635"
     CDS             22506..24305
                     /locus_tag="PKK03_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNX83122.1"
                     /translation="MLKNHPKGLLVAFFANMGERFGFYTMMAILVLFLQAKYGLAAEK
                     AGDIYSWFYFAIYALALVGGILADSTRKYKTVILFGIIIMFAGYLLMAVPGMPLTFTV
                     IALFTIAFGNGLFKGNLQAVVGQMYDNPQYSKLRDTAFMIFYMGINVGAFFAPFAANG
                     VRNWWLKTNGFAHDGSLPALCHQFAGGQITDPAKLQEFQTLADKVNLAGPVSDLGAFA
                     DSYLDVFSRGYNYAFGIAAVAMVISLLVYITFNKLLPNKEKPVKASADSANTLEFEVR
                     PLIYAAIAMAIFAFGLQFLVGWASGLAFGLFAGFVTWILTTAKKDEYARVSSLILVFV
                     VVIFFWMSFHQNGLTLTLFARDYTAKQVGPFTNLFFTLPSMLAVIGSIAGLVMLVRKN
                     MTGTARIIGGALFVCAMVAAYLFVTKADALNSIEPEVFQSFNPLFIVALTFPVMGVFA
                     WLNKKNIEPSTPKKIGIGMLIAAVGFVVVLLSSVKLPSPASLEGSPVAEALRVTPYWL
                     ISSYLILTVAELFLSPMGLSFVSKVAPSRFQGLMQAGWLLATAVGNKLLFVGTKMWDK
                     VELWQLWTVFIVCCLLSAAFIFSIMKRLENATK"
     gene            24429..24659
                     /locus_tag="PKK03_01640"
     CDS             24429..24659
                     /locus_tag="PKK03_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83123.1"
                     /translation="MNSSKMYDKVFAIFGTVMVFFYFGLAIFVLTTKIFDIDKAMRII
                     LAVPLFIYAIYRVFISYEKIRDSFFSNKDGDE"
     gene            24835..25794
                     /locus_tag="PKK03_01645"
     CDS             24835..25794
                     /locus_tag="PKK03_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNX83124.1"
                     /translation="MNKAKSLTAAVAGVIFLALLNLMPGCKNKGTTALKETPTSGTIR
                     IASDESFRPIIEAELDTFTALYSYAHITPLYMPENELVAFFLNDSVKVIVSSWAPTEE
                     QRKLLLDMQTVVRTTVVAYDALAIVLHRSNPDSLLTYENVKDIFTGAVTDWKQINPSS
                     KLGQTNVVFDNVRSGNIRYFREKFTLPEELLPNFFAVKSNPEVIDYVSRTPGAIGIVS
                     VNWISDEDDPQSFGMLGKIKVAAISQPYLDKTSYYPPLQGHIYEKTYPFVREVNMHSR
                     ESYTGLGSGFVSFVAGEKGQRIILKSGLVPATMPIRIIQVKNE"
     gene            25816..28002
                     /locus_tag="PKK03_01650"
     CDS             25816..28002
                     /locus_tag="PKK03_01650"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNX83125.1"
                     /translation="MRSFKLALLPAAVFCMVFGTGLKAQTLDEARTFTKNEQYDKAEV
                     LFQQLIKNEPTNSKIFFHYGENALLNYFADTISNSLNIAMKEAADIFDKGVAANAADP
                     LNHIGLAKVALYNGQNARAEELRLKAKGLLPPYKKVTKIKDPKGYAYALAKIAESYIR
                     FEQVDTSKALPFVRQALSIDPRNSEVYIIAGDIYDLVNDGSKAIKNYNLAQDWDLKSP
                     AANMKIGSIYVKGRNLTAAIPYYEQAIALDQNYAPAYRELGQLYSMAGRFNDSKKYFE
                     KYLELTQGNIPAKIRYVNALFYAKEYDEVIKNVEEIFAVDQSRTYMNRIAGYSAYEEG
                     NFELAKQYMDKLFANLEADRIIKKDYIYYARILARKNQNYSKLMIDVDNDADELSKLQ
                     ARYVTLKSPAKEQAKASIDALQARVEAGRTQVKKAEAEFAKAFEAYDKAIRFNEEEDL
                     NLMYEKGTIQYGAHMYAEAASTWSQLLEKGRDSENDFLQVGRAFYQDKAFDKADQAFN
                     KMIAKYPDNLQGYLWLANIASAKDPDSELGLAKPRFQMLLNKAAADSVKNVKEIYDAL
                     RYLGYEALQNKRYDEAKAYYNRMMNLSPEYRIRAHSSLSTMYMTMGDYPKAIEENNRI
                     LAIEPGNDGAKSAIQYIAQLQKSAQPKAHPNEITGVISDSNGDPIPGASVRVKDTAAE
                     AWTNARGEYKFVMPEASETLVISAKGYKSIEIPVSKRRTYNATLDK"
     gene            28118..28933
                     /locus_tag="PKK03_01655"
     CDS             28118..28933
                     /locus_tag="PKK03_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNX83126.1"
                     /translation="MSKKTKSSSGVWQSLFAPLAIILAFIVAYLIFTRVLGNPVNFEG
                     GNPKGHPLQGNLLGLMYKGGVIVPVLITIVITLIIYFFERLITLTRVKGRARLNVFVS
                     NVQQMLAEDRIEDAIVACDKQKGSLANVLKAGLSRYRALENDKELEKDQKITSMKQAI
                     EEATALELPVLSRNMVILSTIASISVLIGLIGTVIGMIRAFAALAQSGAPDALALSTG
                     ISEALVNTAFGITGSTLAIIFFNWFSSMIDSYVFKIDEAGFSLTQTFAASIRK"
     gene            28978..29616
                     /locus_tag="PKK03_01660"
     CDS             28978..29616
                     /locus_tag="PKK03_01660"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX83127.1"
                     /translation="MPKIKLGHKSPRIDMTPMVDLFTLLLTFFILTATFRPQESAPVD
                     TPFSVSEKPMMDANVMTVLIAKDNRVFFNVDNGPDTILKFRPKILAEMGKRYNIEFTE
                     DELRLFEKTGTAVGVPIQDMKKYLASKDSDEKIALEKGVPIDSTDNQLALWILFARQI
                     NPAADAMIKGDADVEFPVVREVLDILQDKNVNRFNLITNLRSAEVTEEQIAE"
     gene            29618..30277
                     /locus_tag="PKK03_01665"
     CDS             29618..30277
                     /locus_tag="PKK03_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX83128.1"
                     /translation="MADVQVQEQSKGGKKKPKKGDARVDMTPMVDLMSLLITFFMLTA
                     AFSKPKVMEIILPEKIKKNEKVEPPKIADSRTLNIILGPEDKVYWYPGKADPEVTVLQ
                     ETDFSATGIRQVLLDRNRALFRKIDQFEKDVIAGKINIKQDSMRSAISQLKKDDDTGP
                     IVLIKAYKTAKYKNFVDIMDEMSIVGIARYIFTDIDWIEEKLVVDAINRAAATPSGSA
                     Q"
     gene            30292..31071
                     /locus_tag="PKK03_01670"
     CDS             30292..31071
                     /locus_tag="PKK03_01670"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNX83129.1"
                     /translation="MANEKKRYPSFDDIVFEGRNKEYGAYEIRRKYARTMTMSIIIGI
                     IVVFFAAYIPYIKAKSVAQIKQRDANEVIAEMANDINQEAAAPPPPPPPPPPSEQQTV
                     VKYVAPVIVDTIKPEDASQFMTADDVAETVVDEEVGLEVIEQVQEEVQEEAPREVFVV
                     VEEMPSFPGGDTELFKFIYDNIKYPELAKENNIQGKVILRFCVTYKGTVDQVSVVRGV
                     DPSLDEEAIRVIKMLPLWKPGKQGGKPVNVWYSVPISFQLK"
     gene            complement(31144..31986)
                     /locus_tag="PKK03_01675"
     CDS             complement(31144..31986)
                     /locus_tag="PKK03_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83130.1"
                     /translation="MKRNLFISVLLIASFQAIQGQDLFPDLKGYRMSNDYPVYTPDDL
                     WNYINGAADAYLQLGFLDLNIREYVKGRNSIKAEIYRFGDDARAFGIYSMERSPGYKF
                     INTGVQGYNEEGAVHFYKDCFYVKVMSHSRSAKVNSAMLHLAGLISSGIGGSGEFPAL
                     LGLFPSEGLLKNQETYLLESVLGHEFLRGAFRASYEVEGDRFDIYLFSCEDPADAGAM
                     GAALAGDAWSADEEVFKYAFEDGFNGVLHMARQGGKLILVSGLPFERTPLAERYITLM
                     LGGR"
     gene            complement(31999..33183)
                     /locus_tag="PKK03_01680"
     CDS             complement(31999..33183)
                     /locus_tag="PKK03_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNX83131.1"
                     /translation="MKRRNFFRSSLAGAAGITLMGNRALAVNGKTDEGEIIYRTLGKT
                     NLRLPVVSFGVMRSDNSSLVRAAHEGGIKLFDTAHGYMNGRNEEMLGGFFSTLPRESF
                     VLATKVKPAGVDSRTGLPSKDTTAESFLAMFNLSLSRLKLDYVDILYMHSADSVEMVR
                     HRETVKAMLDLKKQGRVRFIGISTHKNEPLVINTMVEDGIWDVVLTSYNYRQTYLAEM
                     NSAIEKAAAAGIGVVAMKTLAGGWLDRERTKPVNVQAALKWVLSNPNVHTTIPGITAF
                     DQLETDLKIMKDIVLTDQEKADLVAEASLPGLYCNACSNCVPGCPNNLPIPELMRAYM
                     YAYGYHNAAMASELVSETAVEANPCAGCDTCTASCVRGFDIRGKISDISRLANVPYDL
                     LG"
     gene            33349..33996
                     /locus_tag="PKK03_01685"
     CDS             33349..33996
                     /locus_tag="PKK03_01685"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX83132.1"
                     /translation="MGRYIHNLISGGEGLNLDFKYCISDPQKIARTLSAFSNTAGGKL
                     LIGVRDNGSLAGVRSDEEYYMIDAAARLHCDPEVTIRTRNHTVNGKNILEVEVPKSNI
                     IPVKARDEHGRWKAYFRQNDQNFMADRVILQVWRRSVKSRGLLLRFEETENLLLNHLR
                     SEGRITVEEFRNLTGINARRAEKILSDFILCGLITYEASERGIFYRLSTEFGSGR"
     gene            complement(34001..37309)
                     /locus_tag="PKK03_01690"
     CDS             complement(34001..37309)
                     /locus_tag="PKK03_01690"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNX83133.1"
                     /translation="MKKGLKVTILLLLSSLSVFSQVNRYGTPLISRFDASATPGELRN
                     LCITMDSRGVMYFGNESGGIVTYDGSRWSRIATPGIGAVTSLITDTRGIVYAGGRNGF
                     GFLQPDNAGRLTYISLAGLTRSGEGNYSPITSGAADSNHVCFSDGRRLFVSGHKSDSV
                     TVTDLENELGIRNVSALLAFDNRIIIADNQEGLFAFSADRTERIPGGEKAAPARFVRL
                     LPYDRDNLLIGDAQKGLMLFNIRTGTIARLPVVTESKTTVPGGSLTDVVLLPGNLIAA
                     GYSDRGGVYIYSHEGRLLQHISERTAAVAESSVTAMYCNNTSNSQLWFCTSGFINRAS
                     ISMPASGFTPAGGITSVCGTVAEYAGAIYAGAEDGLLVSYTDPSGVRRFRRKGSQGAR
                     VYSLAVVELREGSVLLAATENGLLQIDRNDDLERFLPGLHLTAACNSVRNPGVMVSGS
                     PDGTIRTLIYDGYEWEVRHTISNAVTGRISDIEQTAPGEWWLATSDPASLVRMHCESN
                     DTAFIVYGRDSGVRSDTLNSLAVINDSLYLCTGRGICVYDRQNDTFRKDHNLTGETFD
                     DANIRMVTSTPEREIVISGCDSRNFDAMVTTTRQGHVVFRRQFDFLPDLATAGISYAD
                     GSVWLAKGQNLFVMDKSKLSFRYGDFSTLFTRIITGSGLVLMDGTFYTDTKEGQRIPS
                     PIQPDKPGITLPNSDNSLTFSWTTTSYIDEEKTEYRHRLEGYDDDWSAWEKRNYRDFT
                     NLPSGDYRFVVKARTITGFEGEELSYSFSVRKPWLTSLIARLFYAAVAGWLLFLLIRY
                     FARRLRIRNRRLDSLLRQRNEATARGKNEIEGLEKYAGLVQQTLLPSERRLGEAIPNS
                     FLLNRPRGTVSGDFFWIARQGERSIIAAGDCTGHGVHSALRTVMALCFLDEIADRPGY
                     MSTSEILREFRYRLTRAFSTLPAGEVQHEGIDISLLSVDRGRKTVEYSGAAMQCFRVR
                     EMSDQEKARWDNGEFKPNAGTQVSGKYLLETVYGDRVPLGMHLDGDHVFTQHTWKLEK
                     DSSYYLFTDGYPDQFNGVTGKKFMRRNMRKLILDIQNYPMSKQKEILEERLGSWMGKT
                     QQTDDILVAGLRID"
     gene            37434..41168
                     /locus_tag="PKK03_01695"
     CDS             37434..41168
                     /locus_tag="PKK03_01695"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNX83134.1"
                     /translation="MRVKQRATAVLAAIMLLLNLPDADSQAYAGRTAGDKPGVKVSRQ
                     LSDFIYRSGESEFQTITDPSGKYLRLFLPGHHQSPETGQPELPVWSRLVEVPDGMEVV
                     VSISEVSSRRIRFADRGAPQAELFPAQPARTKNQVRDDRITVKDRKAYGSRNTLMHDT
                     VRVTHEGIFRGRRLANIAVYPAFYNPGGRYVDLITSMKVEIGFRPEATKGEEDTEDPV
                     NKGGFASDSYITGYTDRPVHMIIVTDSIFRKHLEPLVRWKTLKGIRTTVIYRKSGPAD
                     TVYKDLKNRISTVYFSLQEKGIPVHYLMIAGDQSIIPTSRGTTNVSDLYYGEFDGQGD
                     YIPELFIGRLPASDTTQMKGMVKKIIDYETNRFGPAIDNWSRALATAGNAPGFELYMN
                     GQVSYIYNTYLKADKSLNAIGWLYPDAPQKDDSLKTVFNKGVSILNYTGHGEATGFSD
                     PAFRTSMMNEFSNENKYPLIIANACRTAQINVASCFGTAMVATPNKGAIGYIGCTNDS
                     YWSDDFFWSVGPGTPGPNVTFETTGAGAFDRLFHTHGEPPGEWYYTMGQVNFSGNMSV
                     SASTSPRKKYYWETYMLLGDPSLSPVIGRPDTFRIDLPDRVPQQLQVLSFFTGPFAYA
                     ALSDFDTLWDARFVSPSGNISLAVPAGVKDSCLLVVTGQNMLPFYKTIHFGDVPEPFI
                     TVGDIVFDDADGNGNGLPDYSEPVNLQVTVKNIGKMRTSKMTARLSVASGMITVEADT
                     ATIGVLLPGQTRTITGKFIFRVSDSVEDGELAALLLSIKDGSEEYRYGIDMTLNAPEL
                     KIISAIHDDSSLGNSNFLPDPGEKLHLRVRVKNDGSAAATGLVKITPAGPMLTVYQPE
                     LATDSIKPGQVKDIYFETEISPLAGSGKVIPYDVSFICGKYEARGRWSVSTGKTRETW
                     EFDRFDVFPWILNGEYPWIITSSVSYENVFSARSAPIPDKTESILAIYVNNPVADTMS
                     FFVRVSSEPIYDELTFRVDSVTDMQISGDTPWAQRKKVLKPGVHLLEWIYSKDVSLSG
                     GLDGAWLDQVTFPDISFLEADLHIDSVFAPPAEAMLSEVTVKGRVINLGRNALTSFPL
                     AYSINEGELVNETFYRKIDPGDTVDVAFTQKCSLLKDIPYHIHIFSRLPEDGYAGNDT
                     AFVSFVISGTGPEINEDQVILHPNPFNEDFSLEVDFGGSETAGFELFDASGRIVMQSE
                     AELSPGRNRIPFNCRHLGSGIYTLRITYGGRSFSMKAVKK"
     gene            41217..>41442
                     /locus_tag="PKK03_01700"
     CDS             41217..>41442
                     /locus_tag="PKK03_01700"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNX83135.1"
                     /translation="MTGKTPTREEAVELFRRYNRSDSLYKHALSVEAVMRYMARKHGE
                     DEEKWGIIGLVHDLDYEMYPDQHCTMTEKIL"
BASE COUNT        10734 a        10789 c        10059 g         9860 t
ORIGIN      
        1 ctgattcaag cttctcgaca gcttttgaca ctacctgtac cctggaattc attgccgata
       61 tcgatctctt cgtggatgcc ggaatggccc tgaaggattc attaagccgt gcaaccagtg
      121 taagggtcaa cactctcacc gttgagctgt attctgtacc ctgggatgaa ttcgagtacc
      181 atggttgttt cgactgtgac tgggacgaga ttgattatca gggcctgtca gaagatcctc
      241 cgcagctgtt cccctttgtc atcggccaga agaactacaa cgcgacatca aaatgcttca
      301 ttctgtacgc caaaaccttc tggtacctga gtcctgctga tgtttccgga ttcatagatg
      361 atgaaccgac agccaatacc ctgacttttg aacagattga gaagggagat ttcacctggg
      421 aagctgctca gctcagcgca ctggagtcag tcatggtcat gcttcccgcc gcagaaagct
      481 gggattcact tgcggtatca gaaaaccggg caatatttca gtataccgta gatgatctct
      541 ttgacggaga gagcctcgaa caaatatggc tccgtgccga ttttccgatt gatgagaatg
      601 tttcgcttcg aacaggaaaa tttacccctg gtaaatgaac ttgctccgaa aggcggcagg
      661 atgtaccatt aaccgtttat ctccaggtca tggacatact tgttgtcgtc agacaggtac
      721 aactcaaggg tgattgaata cggcttcctg atcaccacaa tcttcttaca aggactcaga
      781 gtcttatcat tctttgcctt gcagtaatag tgcctgcaaa tcccgtcaaa acggtcctcc
      841 ttgtcctcgg taagataaac ctcaagacga agcggctcgg tattcccgtt aaggtagttg
      901 gagataacta tctccctgtc actgatgaca atagacctct cggtccggtc aatctcgaag
      961 ccatctctcc tgatggttgc ggccctgctt ttgtaggtct gcgaaaaggc gccgaccccg
     1021 gtcagcacca aaagaagcag aaggatgatc tttttcatgc caaggatttt tccatatttt
     1081 gacttatcca cttatacgga ccaggtcgta ttttgttctg tcatcactta aacaaaacct
     1141 tcgaaaagca tctgtccgtc aatacactgg cgctgaatgt caaaattgac ttccggattc
     1201 tgggtctcca tgataatttt attccagttt agtccgacac catgaaaaca ttcgtcctcg
     1261 cagcaatctg tgctgttatt atcttttcta tgccaataac tgtctcagca cagtcaggcg
     1321 tttctcccaa gaaagttctt gaagctcttg cgggaaggga attcaaacat gcccttgacc
     1381 tggcaggtaa ggtcgatgaa ccaaattatt ccgacaagtc aggtgtgacg ctgctgatgt
     1441 atgctgcaga aacgggagca gctgatgtat gcagggtatt gctggaaagg ggggcagatc
     1501 acagtctccg tactgcaaac ggcactacgg cactctattt ggcagcccag aacgggcaca
     1561 ccggagtggt cagaaccctc ctcgaaaagg gagctgaccc tgacatacgg cctgataacg
     1621 gcgcaacacc actcatagca gcatcagaat atggccacac tgacgttgca gcccttctgg
     1681 ttgaaaaagg ggcagatgcg tccctgcagg ataaccatgg ctccactgca ctgattctcg
     1741 catcgcagaa cggcaatgaa gaaatagtca ggatcctgtt aggcaagggc attgatccgg
     1801 atatccagga cttgaacgga tccactgcac ttatcctggc cgcccaggaa ggacagactg
     1861 aaatagcaag gatgctgttg caaagcggcg ctgacgtcaa ccggcagacc ctgaacggca
     1921 attccgcgct gatactggct tcattgaagg gcaacatgga aaccgttcag atgctgctgg
     1981 acagcggagc accggtagac acgaagacca ccacgggagc cactgccctc ttcgcagcag
     2041 cctcacgcgg acattgccag atagtaagct ttcttctcga aaagggcgct gaccctatga
     2101 taagggatac aaaagataaa actgcctaca tgtatgccaa ggattacaat gtcaagtctg
     2161 tcctgacatc ctggagcaaa aagtaactac tcacttcata agcctctcta tctcatcagg
     2221 ttccttcggg atggatgctg aaagtacagt gttgccggta ccggtgataa gcacatcatc
     2281 ctctatcctg acgcctatgt tgctatattt ttcgtatacg ggcctcacct tcgagatgaa
     2341 atcggcaatc tccccttccc cggcttcgct gccgtaaagc agctcaagct gatcaagccc
     2401 gtttgcccgg aggtagatac caggctccac cgtaagaacc atacccgcct caagtggtct
     2461 gcccactgct gtatcagcta tgagataact gctcatcatt gaccgtggta ttccctgggt
     2521 cccggtatca tgcacatcaa gtcccaggaa atggcatata tggtagacag tgtagaattt
     2581 gcgctgccac tggcagtcag ggtctgtgac caatcccagg ctacggaggc cgtcaataat
     2641 agtgttggtt gccatcctgt gtgcagcggt catcggcgca ccaggcttca tctcgcctat
     2701 cgcagccttc tgggcccgca ataccaggtc ataaatatct ctctgctcct ccgtgaacct
     2761 tccgttcacg ggaacagtac gggtaatgtc tgcagtgtag tatccgtact cagcaccaac
     2821 atccatcagc agcaggtcgc cctcttccat cttcctgtta ttagccgaat aatggagcgt
     2881 gacggcattc tcccccgaac cgactatcga ctcgaagccg ggcatattgg aaccgttgcg
     2941 ccgccactca tattccagaa gtgcttccat ctcaaactca aacattcccg gagcgcactc
     3001 agccagcact gcttcgagac ccttgccggt aatgtcaaca gccttctgca gcatcattac
     3061 ctcatattca tccttgtcaa ccctcatctc tgacagcgcc ggattgatat ccctgagcaa
     3121 tgcaccggca gaattctcag ttcccagtat ctcctgcaca tgatccctga agacgcggtc
     3181 gcggaagtca aggtagatgt gtcttccctc ccttgcagct tcggcaatgt tcctgtcagc
     3241 ctcacggaag tcaagtacgg tatcaggagc ccatgttatt aaaagttcac ccgggtccgg
     3301 gaggtttccc gaatagatga tttcgcgaat gcttctctcc cttgtataaa gggtgtaagg
     3361 ccgcgcatcc gtgcctgaca gagacataag agctcccggc tgaccgtatc ccgtcaggta
     3421 ccagaagttg cctgctgccc tgtattcatg cctgcctccg tcaaacccat aatctgaccg
     3481 gagtataagc aaaccgtcac caatagtatc aagtatagct tcccgcctca tagagaagac
     3541 tttctgaagc ccggcatcca tcgtcaggtc acctgtatca ggtgcctcag gtgtcgagtc
     3601 atgttgctgc ccgcctcggt ggcaggcaac cacgaaaact gtgataagaa ggagaatgat
     3661 taaccttttc attgaacaat tgttgtatcc ccaaagatat tccaaatcaa tccggaaata
     3721 ttggtttaat ttgttttcaa ttgctaataa atcttaatta tcaaccctta caagccatga
     3781 caatgaacag aaccgccatc ctattcaccc tcgtctccat tctgatcctg tctttcaggt
     3841 cggacaagcc cgcatataac ctttatgatt ctgagggaaa gaatattaaa tatgaaaaga
     3901 tgctggacag aatcagctcg gcagatgtcg tcttcttcgg ggagctgcac gacaatccca
     3961 ttgcccactg gcttgaatat gaggtcactg cagatctcca taagattgca ggagagaggc
     4021 ttgtgctggg cgcagagatg ttcgaggctg acaaccagtt gttgcttgat gaatacctcg
     4081 gatcagaata tgaagctgac aagttcgagg ctgaagttaa actctggaag aattacagga
     4141 cagattacag gccgctggtt gagtttgcga agaaaagcag cctgccattc atagcaacca
     4201 acgtaccgcg caggtatgcc tcaatggtat cccgtggcgg atttgaggag ctggactccc
     4261 tttctgctga ggcgaagaag tatcttgcac cgttaccggt ttcatatgat ccggagctga
     4321 agtgctacaa ggatatgctg tcaatgcacg gtatgccagg gatgggcgcc agaccaaacc
     4381 agaacctccc aaaggcccag gcaatcaagg atgccacaat ggctcacttc attgcggaaa
     4441 acctgccatc gggaaagatc tttatccatt ataacggagc ataccactct gacaactacc
     4501 tggggatagt ccactacctg aaactttaca ggccgggaat aaaaatcgca accattacaa
     4561 ccgtgctcca ggatgatatc actgaccttg aagtggaaaa caggggcctg gctgatttta
     4621 taatagccat acccttgacc atgacacgca catactgacc ctgctccgtc cggacaaaag
     4681 cggaatcttc ttcaatattt aaatatattc tgatccacat atccatatat ttattttata
     4741 tctatctttg tctggttaaa ccaacaggat catggatata acagcaaaga gcaatgtagg
     4801 agacgttgtg cggttcaact tccgtacgtc gtcagtattt caggcaaaca acatagacta
     4861 ctgctgtggc gggaacaaga ccatagatga agcctgcaaa gagtcaggag ccgattcaga
     4921 cagactgatt gccgaactac gcagtacggc agagagcaca gatcccgaca cggaatacat
     4981 caacagcctg tcactgtctg agctcagtga ttatattatt cgcaggcatc actcctttgt
     5041 aaggaagaac atcccgttcc tgaaagaggg gcttgacagg atatgtgagg ttcacggcaa
     5101 tgcccatccg gagcttttcc ggataaggga actgttcaat gagtcatccg gagacctgac
     5161 aattcatatg cagaaagagg agatcatgct gtttccgtac atacagcaga tggaagcatc
     5221 agcccgtggc ggttcaccgc tagggaaacc ccacttcgga tcagttgcga atccggtcag
     5281 gatgatgatt tccgagcatg agaccgaagg ccgcaggttt gatacgatag ctgagatttc
     5341 aggaaattac agccttccgg gtgacgcatg taacacatac agggcaacgc tctccgggct
     5401 gaaagatttc gaaacagatc ttcaccgcca tattcacctg gagaacaaca ttctgtttcc
     5461 gaaagccgtg gaaatggaat caggacttat cgggaaacag tcctgacatt atttttcaga
     5521 gcaataaacc gcagagcgaa aagcaccgtt cttcctgacg gtcattcttc ctgaactcaa
     5581 aaataaatat ctttgggttc ttattccaga accatgcaga ccagagtcag agagatcctt
     5641 gaccaggagg ctgaggccgt caggaagata cctgttacag aaagctacag ccaggcaatt
     5701 gaactgatgc acaaacatgt gcacctgctt aaaggcaaac tggtaacaag cggtatgggt
     5761 aaagcgggac agattgccca tgacattgcc actacattca gctccaccgg aacaccggcg
     5821 gtcttccttc atcccagtga agcccagcac ggtgatctgg gcgtgctcca ggaaaatgac
     5881 cttatgctgg ttatttccaa ttcaggacgc acccgggaga tcctcgagct ccttgaactg
     5941 aaagacaacc tcttcccgaa catgcctgtg gtggtaatct caggcaacaa tgcttcgcca
     6001 ttatgggaac gggccagcgt gggcctcttc acaggcgggc ctgccgaggt gtgcccgctg
     6061 ggactgacac ccacaacctc aaccaccgtc atgaccgtca tcggtgacgt tctggtggcc
     6121 cttatgattg aaaagatcgg cttcaccgcc gcagaatatg ccaaacggca ccatggcgga
     6181 tacctgggtt acaaatcaag ggaacgctcc ggatactccg gaggcaccat ctactgaacc
     6241 agatcagcaa ctccctcgag tatgtatgag ggcctgtagg caaacttgtc agccgtcttg
     6301 agggttgata ttccggtcag caccagcagg gtgtctatct ctgactcaat gccggctatg
     6361 atatccgtat ccatcctgtc gcctatgatg atcgtctcct cgtttgagca ccctattttc
     6421 ttcagggcta tcctcatcat cagcggatta ggtttgccta cgaagtatgc ctgcctgccg
     6481 gttgagagct ctatcggtga gatcagagcc ttggtggccg ggataatgcc attctccacc
     6541 gggcctgtca ggtcaggatt tgttccgatc agcttggcgc ctttaagcac caggttgaca
     6601 gcctgttcta tcttttcgaa actgtagctt cgggtatcac ccacaaccac ataatcagga
     6661 ttgtagttat tcattgtata cccggcattg tacattgcgt tgatcaggcc tgcctctcct
     6721 actatgtaag ccgttcctcc cggcttctgt gtggccagga actgggccgt ggccagcgca
     6781 ctggtataga aaacattctc gtcaagatct attccgagcc tgagcatctt ttcctgaagc
     6841 tccttggggg tcctttcact gctgttggtc aggaaaagaa atttcttccc ctccttcttc
     6901 agccactcca caaattccgg aacgcccgga agcagtttgt tgccatggta tatcacgcca
     6961 tccatgtcac tgataaaggc cttcttgctc tttatccttt caataatctc actgtttttc
     7021 atcattctga tttctggcgc aaaattaaca cttcgctgtc agattgtaat acaggggtgt
     7081 ggccataaat acagacatac gttgagaatt ttgttactca accaggctgc atgccggcca
     7141 gggcggcctc agaccggaag gaaccattat aaatgggaag tgatattgca gtttatttcc
     7201 aaaaagagta gttttgatgc cggcccgacg caaatttcac tgcagcggca aaactaacca
     7261 caaccttaac ataccataaa caagatgacc acaggtgcaa caccggcccc catgagccgc
     7321 aagaattaca tcgttggaat gacggtaatt gccatattct ttttcatctt tggtttcata
     7381 acatggctta acggaatact cattccgttc ctgcgcacag cctgtgaact gactgacttc
     7441 caggcatatt ttgtcactac agctttctac atctcttact tcgtgatgtc actgccatca
     7501 tccgcggtgc tcaagaaaac cggctttaaa aacggcatgt caatcggcct ctggattatg
     7561 gccgtgggtg cactgatatt cattcctgct gcgcttgcgc gaaactacgc catcttcctc
     7621 cttggtctct ttgtccaggg taccgggatg gcccttctcc agaccgcctc aaatccgtat
     7681 gtgacaatca tcggtccgcg tgatagcgcc gccaagcgaa tcagcatgat gggtatcgcc
     7741 aacaagttcg ccggtgcaat agccccgatc atcctcggag cacatataat gaaggactcc
     7801 cacgtgcttg accggcagct ggccgaagcc accgatgccg ttatgcgggc aggcatcctt
     7861 gatcagctcg ccacccgggt tatcaatcca tatataataa tggcagtcat actcgtggcg
     7921 ctgggactac tccttcgctt cgcgcacctg cccgaagtgg ataccgatgc ggaagaatcc
     7981 tccgttggcg aaaccaatgt caataaaagc agcatatggc acttccccca tatgctagtc
     8041 ggcgccgttg ccctcttctt ctatgtaggc gtcgaggtca tcgcaggtga caccataata
     8101 cggtacggac agtcgttgca gattgacatg tcatcagcaa agtacttcac ttcgctcacc
     8161 atgctgggaa tgatcatagg gtatatctgt ggtatcatct tcattcccag aatgctctca
     8221 caggtaaatg ccctgcgcat atctgccata cttgggatca tcttcacctt cggcgccgtt
     8281 ctctcccctg ccgggcagca gatcaccatg ccattcaggg accttgtaac cttccaggct
     8341 gtgacgctca ccatcccggt tactgtgctg tttgtagcac ttctcgggct agccaacgcg
     8401 cttatctggc cgtcaatgtg gcccctggcc cttaacgggg tgggccgttt caccaaaaca
     8461 gcttcagcac tgcttgtgat ggccgtggta ggaggtgcaa tcatacctct catctacggt
     8521 aagctggctg acaaattcag tacccaggct gcttactggg tctgcattcc ctgctacctt
     8581 atcctgctct actactcact gcacggatac aggctgaagg gaagatagaa aaccaccatg
     8641 gaaaccgatc tgaaaagcat attcagccgc ttccgcggcg aaggcacatt tcttgaagga
     8701 aagtctttcg gcccgggcca tatacatgac accttccgga ttataaccgc tgagccggag
     8761 tgtgacaact acatcatcca gcggctgaac agcaacgtct tccgtgacat cccaaacctc
     8821 caggaaaaca tggagagagt caccggccac ctgaggaata aaatcgcagc catgccgggg
     8881 agtgacccga aacgggagtg ccttaccctg gtgcctgaca gggagacagg aaggtctttc
     8941 atcaccgacg cagatggacg ttactggaga atgttcgtat tcatcgaaga ccacaggtca
     9001 tatgaaaagg ttgactccgc acataaggcc tttgagggcg gtagaatcat cggaagattc
     9061 caggccatgc ttgccgatct gcccggagaa cccctgcatg aaacaatacc tgactttcat
     9121 aatatcgcca ggcgccttga tgcctttcac agggtgatca gccgtgatcc cgccggcagg
     9181 gtggcagcag tcaggtcaga gatagaagag attaccagcc gcgaggagga aatgaaaaca
     9241 atcatcaggc tgggagccgc cggaaaaatt ccgctcagga taacccacaa tgataccaag
     9301 ttcaacaata tcctcttcga caagaatgac agggcgctgc tgataatcga tctcgatacg
     9361 gtgatgccgg gttatattca ttacgacttc ggagatgcca tccgcacagc cgccaaccgg
     9421 acagaggaag acgctgaaga cctgtcagcc gtctcgatgg atatcaatat ctaccaggca
     9481 tacgccgaag gcttcctttc ggaaacaagg aatacgctca atgccactga gatcgagtac
     9541 ctccccttcg cgccaaagct gcttacttac ataatggcaa cacggttcct tactgacttt
     9601 attgacggtg acaactacta caagacccgg catcccctcc ataaccttca gcgcgtgagg
     9661 acccagatca cccttctccg cgacatggag gcccggtatg acaatatggt tcagataatc
     9721 aaaaacatta caaaatgaag aaaatcatac tgctgttcat cgccgttttt ggcctgacac
     9781 tctctgccag ctcacaggca gagaccgcgg tgaccgacag ctcgaaattt gccgcctact
     9841 ggtggcacag caaggatatg tatgaccacc ttcccgatat caggaacgag atagtattcc
     9901 ttgggaacag catcactgac ggagccgagt ggttcgagct ccttgacaac aaaaaatgcc
     9961 ttaaccgcgg catcagcgct gatatcacag agggaatact cctccgcctt gacgccataa
    10021 caaagctcca gccgaagaaa ctattcatca tgatcggcgt caatgacctg tcacgcggca
    10081 tgacacctga cgagattatt gtcaactaca ggaaaatcct cgacagggtc aggaccgaat
    10141 cgccacgaac aaaggtatat gtccagagcg ttctgcccgt taatcccgcc accggtcagg
    10201 caaagaacca caccaacaaa actccccaga tcatggagct caatggcaaa ctgaaggagc
    10261 ttgcagccgg attcggccat acctacattg accttttcag cctgatggct gacccggaga
    10321 atcaccttcc gcgaagcttc agcgttgacg ggcttcatct cacatacaag gcttacaaga
    10381 cctgggctga cgccatcaaa ccgtacgtga aataattttc tgttctactt ttatctgcct
    10441 gttttataaa cattaatggc ctggaatctg ttttcgttcg aaaatagatt tgcataaaag
    10501 gggcaaaata atgacttttg tcatgccgtt cacacggcat gagggtcaca ttttgtcaaa
    10561 gattcatcca tggaaataaa cgggaagtat ttcaggtcat tcattgaaag agaggaggcg
    10621 ctgaagaaac agtacagcgc tgaaagaata aaaaagcagc actccaaggg gaaactgact
    10681 gctcatgagc gtatcgcact tctgtttgac gatggcacct tcgaggagat agatgcctat
    10741 gtcaccccct cagaccaggg tgtggaattc ggcagaatgg aactggcata tggcgacgga
    10801 gtggtcaccg gccacggaaa gatagacggc aggcttgtat ttgccttcgc gcaggacttc
    10861 accatcatgg gggggtcact gggcatcgtc cacgcccgga agatagccaa ggtgcaggag
    10921 atggcgctga agatgggggc gcccttcatc ggcatcaatg actcgggcgg ggcaaggata
    10981 caggagggta tagcaagcct taacggttac ggagtcattt tcaacaatat tatccagtcg
    11041 tcaggcataa ttccccagat atctgtcatc atgggaccgg cagcgggtgg agcggtgtat
    11101 gctcctgccc tgactgactg ggtgttcatg accagcaaca caagctatat gttcgtaacg
    11161 ggacccaacg tggtcaggga ggtgcttaac gaggagataa ccagcgagga gcttggcgga
    11221 gccgaggtgc atgcgaggaa gagcggtgta gcgaacatga tctatgatga tgaggaaaac
    11281 acaattatcg ctgtacgtaa gttcctctcc tacctcccgt cgaacaacct ggagaatcca
    11341 ccggttgcca catatgctga tgaaacgccc ggaataaatc cgaagctgcg cgatattgtc
    11401 cccgatgacc ccaacaaggc atatgacgta cgtgacgtaa tcaaccagat agttgaccgt
    11461 aacagcttct ttgagacctc cgagctcttt gcaggaagcc tggttacagg attcggacgt
    11521 cttgagggaa aaaccatcgg cattgtcgcc aaccagccaa aggtgcttgc aggagtgctg
    11581 gacatagatt cttcagtcaa gggcgccagg ttcatccgtt tctgcgatgc attcaacata
    11641 ccgatactta ccctggagga tgtgccggga tttcttccgg gaaaggacca ggaacactca
    11701 ggtatcatcc ggcatggcgc caagatgctc ttcgcatatg cagaggcaac agtgccgcgc
    11761 atcacggtga tactgcgcaa ggcatacggc ggtgcttaca tcgtgatgaa cagcaagggc
    11821 atgggaggcg acttcaatta cgcatggccc tcagccgaga tagccgtgat gggtcctgac
    11881 ggagccgttg caatactata ccgccgtgaa cttgccgagg cgaaggaccc ggtggcactg
    11941 aagaaggaac tggtgaagaa gtaccgtgag aaggtggcta atccgttcat tgccgatgag
    12001 aaaggtttca tcgatgaggt cattgatccg gccctgacac gtgaaaaggt gctttctgct
    12061 ttctctgccc tgagcaacaa gagcgtaaag gtaccgtcac gtaaacatgg gaatatgccg
    12121 ctgtaaggga tttgggattt gcgatttgcg aattgcgaat tgcgagtagc gagttgcgag
    12181 ttgcgttttt tgaattgcca gactggtata aagaattgta aatgaagaaa atcacccgta
    12241 tattgatagc caaccgcggc gagattgcgg tccggataat gcgcaccgcc cggaaaatgg
    12301 gcattgaaac tgtcgccgtg cgcaccgctg ttgaaccgga cgccatgtac ctctcccatg
    12361 cagacttcat ccatgaagag gatgatgaac tggccgagat acccgtattc ctcgacgttg
    12421 aaaaactgat cgccatcgcg ctgaagacaa aatgcaacgc ggtgcacccg ggctatggtt
    12481 ttctggctga gaatgcatac tttgccgaga gatgccgcga caataagatc accttcatcg
    12541 gtccatcacc ggatgccatt tacaagatgg gcaacaagcc ggtggcccgg cagatcgcaa
    12601 tgaagcacaa aatacccatg gcaatgggtt caaccggcag cgttgcaaat gaagacgaag
    12661 ctgtaaggca cgcagccagg ataggctacc cggtcatcat caaggctgcc tccggaggag
    12721 gcgggcgcgg aatgcgcatc gtcaggatgg agtccgaaat ggagaagatg ttcatccttg
    12781 ccagccgcga agccgaaaaa gctttcaatg accccagcgt ctttattgaa aaatatatag
    12841 agaatcccaa acatattgag ttccagatac tgggtgacac ccatggaaac attatccacc
    12901 tgggtgagag ggagtgctcc atccagcgga aacaccagaa gctcctcgag gaggcaccat
    12961 cgccggcact cgatgaggag ctgagggcac agatgggtga aacagccgta agaatcgccc
    13021 gcgccgtagg ctattactca gccggaacgg ttgagttcct tctcgattca gaccgcaatt
    13081 tctacttcat ggagatgaac acccgcatcc aggttgaaca ccctgtcacc gagatgatca
    13141 ccggcatcga tctcattgaa cagcagatac ggatcgcctc cggcgaagag ctgaccctca
    13201 gacagaagga tgtaaaactt gccggatggg ccattgaatg ccgtataaac gctgaggatg
    13261 tacaggcagg ctttgctcct gcaccgggaa aaattgaaaa actgagcctc ccggaagggc
    13321 cgggtgtcag gattgatacc ggcgtacggg ccgggtcatc catcgtggca tcatatgact
    13381 ccatgattgc caaactgatc gtcaccggca gagaccgcca tgaggcgata atcaacatga
    13441 agcaggcgct tgacaagatc tggatcaagg gcaccaagac aaccctcccc ttcttccgga
    13501 tgctgatgcg ccaccaggct ttccaggcag gcaccttcac cacctctttc attgaaaagg
    13561 accttgacag gtaccacttc agcagtgagt acgaagagac catcgcagca tggatagcca
    13621 caaagctctt tacagaggaa aacctctcat caggcaaacc cggcataagc ttcgaccagg
    13681 gcagggaact gacaccctgg ctgctccgaa aaagactgac ccagttttag ccgtgaccaa
    13741 acctcagcag aaccgcaaaa caggatacat ttaaaacccg aaaacagaga gatgacaccc
    13801 gaaaaacctg caataaagaa actgtatgcc ctttccaatg agggccgtaa gtttgccttt
    13861 tcactgccac tgaagaacgt ggtaagggta aacggacggg actacagtgt cgaacttcgc
    13921 gaagacgaga aatacggagt gcacgtgcta tggaagaaca ggcgctaccc cgtggagata
    13981 gtgcggacga agcagaacag gtatgagatc ctcttcaatg acatcagcca cacgttcaca
    14041 gttgagactc ccgtttcaat gcagagaaaa aaggccctgg gaactgcaag ggagaaaagc
    14101 acgaccctga ttgtaagggc acccatgccc ggaaggataa ttgacgtgct tgtccgcgaa
    14161 ggcgccgagg tggtacgcgg agaagctctt gtgatactcg aagccatgaa aatgcagaac
    14221 gagattcagt cggttgtcag cgggactgtc gtcaggatcg gagtcagatc aggccagaac
    14281 gtgatgaagg atgatatcct ggcggagata aaagtcagat agccgccatg gcgttgcccg
    14341 gtgtcagaat ttcatgcctt aggtgcattc ttaagggttg ccaccaggaa attccagaac
    14401 ttgccgaccg attcgatttc caccttctcg tcaggactgt gcgggtggcg gatagtcggg
    14461 ccaaagctga ttgagtcaag cttcggatag acacccatga ttataccgca ctcaaggcct
    14521 gcatgaatgg cttttatctc gggcaccctg ccatagagct tattgtaggt gtccttcatg
    14581 gcactgagga tggcgctgtt catatcaggt ttccatcccg ggtagcttcc cgagagttca
    14641 acctcgcagc ctgcaagctg aaagcctgca gcgatcttcc atgccgtggc ttccttggca
    14701 gactcaacag agcttctgca gaggcagaaa gcctcgaact tgccgtttcc tatcctcact
    14761 atggagaggt tatttgatgt ctcaaccaca cccggcatgc tggcgctcat ccgctgaacg
    14821 ccattggggc atacgaacac tcccctgatg atgcggtact ggtctgcttc attcatcacc
    14881 ttcgcaggtt catcggcaac cttgaaaatc agcttgatgt caggatcaac ttccttgaat
    14941 tcagctacgt acatcttttc ataacccttc atgaatttct cgaactgggc ttttttaatg
    15001 gaaggcacaa caattgttgc gaatgactcc ctggggatgg catttcgcag gtcaccaccg
    15061 ctgtattcgg ctatgcgcat tccgaagtcg cactcagcct gctggaaaaa cctgaacata
    15121 agtttgttgg cgttagcctt gccaagattg atgtcaagcc ctgagtgacc gcccctgagt
    15181 cccttaaggc tcagtttata ggccgtcatt ccctgtggcg tcttctcctg cttgtagctg
    15241 cgcgttgctc ctacgtcaac gccgcctgcg catcccacat aaagttcgcc ctcatcctcc
    15301 gagtccatat tcagaagtat ctctcccttg agcatgccct tcttcaggtt cttcgcacct
    15361 gtcataccgg tttcttcgtc aaccgtgaag agtgcctcaa gcgggccgtg ggtcagtttg
    15421 gggtcagtca tcaccgccat ggcggcagct actcccattc cgttgtcagc gccaagggtt
    15481 gtcccttttg ccttgaccca tcccttgtca atccaggtct caatgggatc tttctcaaaa
    15541 tcaaatttct tgtcggcatt cttctgcggc accatgtcaa tatgagcctg aaggacaatt
    15601 cccttgcggt tctccatccc ctttgtggcc ggttttctga agacgacgtt tccggttttg
    15661 tcaacgatgg tctcaaggcc gtgttcctgc ccgaacttct tcatgaattc cgtcatcctg
    15721 tgctccttct tggacggacg cgggatctgt gttatttcat agaaatattt ccacagaagc
    15781 tttggttcaa gatttttaat ttcactcata actatttctg tttttgatta ttcttttaca
    15841 atgtttggca tctcgagccc gtagcccttc tttctgtctt ctgccttcag caggaagcca
    15901 aagagtatgg ccagaattcc gagtgaggtg aaaatcatca tcgggacctg gtagttgtag
    15961 gtaggcactg agataccgtc aatcatgcgt gaacccgtaa tgcagtattt gtcaagaacc
    16021 cagccaataa gcatcgggac acccatgagg ccccagttct ggacccagaa gataagagca
    16081 tacgccgtcc ccaactgctt catgggtatt atcttgggta ctgatggcca catggctgaa
    16141 ggcacaaggg aaaaaccaat cccgaggata attatcagca gtactgcctg ccagggttga
    16201 ttgaacatag gtattgagaa catggcatga acgaatatca ggagcacagc gccgatgatc
    16261 atgattgtgg ctccctttcc tttcctgtca tacaggttgc cgaagaaagg ggtcagcacc
    16321 attgttccca gaggcagtaa agatggtata atccccgcag tttccggatc aaccccgaac
    16381 ttgtttacca tcaggtcaga tgcatatttg aggaacggaa atacagccga gtagaagagt
    16441 acgcacagaa tggcaataaa ccaccagccc ctgtttctga ggatgaatcc gatatcctta
    16501 agctggaagc tctcctccgc ctctttgacc atccctttgt ctgcctcggc gattgatgcg
    16561 tcaagcttct tatccatacc gcagtagata aagaacgcta tcatgcctat gcagagcaga
    16621 ataagggcca taagaatcgg catggatacg tgtccgagag ccttggctat gggggctgaa
    16681 gtctgaagtg caagcatggt gccgagcctg gctgttgcca tttcaaggcc cattgcaagt
    16741 gccagttcct tacccctgaa ccacttcaca atgattttgg aaactgtaat acctgctgtc
    16801 tcaaccccta ctccgaagag cgcaaaacca aggctggcaa ccagtacctg cgtcttcatc
    16861 ccgaaaagaa tctgtccgtc cagcatattg gttgatacag cccagtactt gattgccgta
    16921 ccgatgacca tcaacagtgt tgccatcttc cccgtgaaac gtacacccat cttgtcaagg
    16981 atgatacccc cgaggatgag catgagcagg aagacattga accagccgta agcacctgta
    17041 aacgttccgt attcgctgct tgaccatgac aactgctgct cgagcatcgt cttaagggga
    17101 gccatcacgt ccgtcaggta ataaccgcag agcatagtaa atgccacaac cgcaagggca
    17161 gtccatctcg ccgccttcga ttcagcaagg gtctttctga ttttttccat tttaatattt
    17221 cgagtttaac atagaataca tggtctgtaa atgtataaaa aattagttcc aataattgcg
    17281 tcaaaaaaca tggacctgaa ctttgtggca ttattttggt tattggactg aaagactaat
    17341 tttgtgcaat tatcgttaaa ttgttcaacc ggatataatt aatgatctat atgaagaaac
    17401 tgatttttgt aatgttactg gcttcagccg ggcttgctgt ttccgcacag gaaccggcag
    17461 tgaagtggta ttcgatagag gaagctgtag ccctggcaaa aacaagcccc aggccaatct
    17521 tcgttgacgc gtacaccgac tggtgcggct ggtgcaagaa actcgacaag gatacttttt
    17581 caaatcctgt gatcgccgag atactcaata ataaatacta tgcggtgaaa ttcgatgccg
    17641 aaggcaagga acctgtcacc ttccagggca ggaaattcgt caatgacgga agccttggca
    17701 agactcatca gctagcctac gctctcctgc aggggaaact cggttacccg accgtggtgt
    17761 ttcttaccgc cagcagtgaa cttatcactc ctgtgtcagg gtataaaacc cccgctcaga
    17821 tcgaaccgat gctcagctac ttcgccggca ccagctggca gaaacagagc tatgatgatt
    17881 tcctgaagac cttccaggga agagtgcagt aatttacctg tgccttttct gaaggtgtac
    17941 cttagccgga cacaaacctg acataaagaa cacaacagcg ccatgatccc tgaattagcc
    18001 cagtcgcctt accagatcac cccgcaactg gaagacgcaa tgagcactac cgtcaaatac
    18061 ctcggaagaa aggttaagcc ctcttcctcc atgctcgatg tgggtcagaa aaatcccctg
    18121 accgaactgc tcgaaaaagg ggacatgaag ctgaagatta caaacacgga aggtgacctg
    18181 gatgtattca cacttcatga caccaatatt tacgactata ttctatacag tcatactatt
    18241 gagcatcagt ttaatccact tcacaccctt ctggaactga aaaaggtgat gtcagataaa
    18301 acgctgatgt tcataatact gccgcgaaga ggaaaactcc tgtggtgcaa gggccatttc
    18361 cacgaaattg atgactacag gatgaagctg ctgcttgagc gggcaggact tgatgttgta
    18421 agccttgaac tgcacaaggc atggagaccg tggtattttt acctcaccgg tttcaaaccc
    18481 atcatgagat tgtttttgga gtacaacgct tactatgagg tcagactgca cagataatct
    18541 cacggaagca attgacttga tactattctc tgcggaacta accgcgtctg tcttttaact
    18601 ttgatgggga acaagatcct atatattttc ccggaaagtt caactttcat cagaaaggat
    18661 atcggctttc tgtcaatcca ttacagggtt ataacccctg aacacgactg gaaaataaaa
    18721 agccaggttc cgctgaattt tatcaggcag tttttctttc tctgccggaa tctccgcggt
    18781 tccggagctg tattcgtaat gttcggtgga tactggtcat tccttccggc actgacggga
    18841 aagatcaccg ggatacccgt atatatcatt cccgggggaa ccgactgcgt ctcatttccc
    18901 tcgcttaact acggcagcct gaggaaacct gtgatgcgaa ccttcatcag atggtcattt
    18961 cagctctgca cgaagctgct gccggttgat gagtcacttg tgctctccgg atacacttac
    19021 ctgcctgacg ctgattaccc gaagcaggga ttcatgtact tcttcccccg gctcaggacc
    19081 ccttaccgcg ttgttcacaa cggcttcgac ccggatttct tcagcactga cccggcagca
    19141 aagagggctg acagcttcat tgcggttgcc ccggtgagca atatgacgcg ggtcagggtg
    19201 aagggcgttg acctggtcat ccatctggcc aggagattca ccggctgcac ctttaccctg
    19261 gtaggcgttg ctgacgatgt tatcagggct ctctcccccc tgccttctaa ccttgtagtc
    19321 tatccctggc tgccaaaaga gaaattcata ggcctgcttg ccgggagccg cttcgttctc
    19381 cagctgtccg tctcggaagg attcccgaat gcactctgtg aagccatgct ttgccactgc
    19441 atcccggtgg gttctgccgt gggagccata ccttcaatta taggtgatac cggatttgtg
    19501 attgccagcc ccgaaaggaa gtacctcgaa aagagattcg aagtgatact gggcacagaa
    19561 tccggggagc agaaccggct tggcatcatg gcacgcaaca ggatagcaga gatgtatcac
    19621 atatcccgca gggaaaaagc tttcctcgaa atccttgatg aaatcccggg ctgaggggaa
    19681 ggtaattctc cctccgctac ctcccgagaa tgtaatttgc aggaagtgtc accaggctgc
    19741 cgtactggta gatcagcctc cgggcagtac agagattctc cccggcaaga gaaacgttta
    19801 tctccgccac gtccggcacc ctgtaataca acatgtcagc tgcattggcc tgtttctgcg
    19861 gggaggcggg tctcacaacc atattcagct ccctggtctt gcctgaaggg gtaatctcca
    19921 tcagtaccgg ttctccggga ccgtctgcag aggtatttac ccctgctgtc tcagagaagg
    19981 tgaaaatggt ggtcttcctg ccagccatct ccgggtcggg agtgacccag atccggaagt
    20041 gcctcgtctc agtgaggctc ttgccggcaa acagcgccat atactccctc tcgagcctgt
    20101 ttatctcatc aagcgcggct ccgtcctgcg ggaagacatt tgtttccccg gtcaggatca
    20161 tatgcctccc ctcgcgcagc tcgagcaacc tgtcggcagc ttcacgggcc tcttccgcca
    20221 ctgacattcc ctttttcttc tgaacaaggt aaggcaccct tatgaaagca gtgtcaacct
    20281 tcaccaggcg gtatgcagtg tcagtcttgg tagcaacata ctcataggct cccctgtcag
    20341 ggaagagcac tcccgcataa tcagggcctc catgctgttc cgaatgcgtc gtacggctgt
    20401 acacgtcagg attgatgtca agcaccaggc cgctcttcct cagagccagc atgtttgtct
    20461 gcatcagggc agtcccctga ataatataga actgtgacgg gtcaagctct tccacggcac
    20521 tcagtcttac accggccagg gtccatttct cggtctgtac ggtaatgaca ttgtccaggc
    20581 caatcagctc agatgcatac ccggcatagg ggccgggcac ctctgtacgt ttttcagcta
    20641 aaatatcaag ctcgaaaaca gtcatcggca gcgcgtagac caggcatccg tctgtaacgg
    20701 tggtcttgtt gccgagcgga ccagccacag tgtcacccgg ggattttccg ctgaacaccg
    20761 agcagcctgc aatcgtcagg gtaagggcag caagaagtat tacagtctgt tttttcatct
    20821 caaccattaa agataaacgt tgaagttgtt aaagtttgtc tcccggaagg ccctcccgat
    20881 attcatcagg atgtcacccg ctatcaccga ctcctctgaa tggatccgca gggctatgtc
    20941 gcctgcaagg ccatgcagat atacacctgt cactgcagcg ccggaggggt catatccctg
    21001 tgccagcagg gaagtgatca tccccgtgag cacatcgccg ctgccgcctg tggccattcc
    21061 cggattgccg gtactgttga agaagaccct tccgtcaggc agagcaacgg aagtgtgtgc
    21121 tcccttgaga accacaacgc accggtgcct tacggcgaag tctttttgtt cctgcatgag
    21181 cctgaagtca gtgctctctg tacccgtcag cctgcggaat tccccggggt ggggtgtcag
    21241 tacagtctcc ggggggagca ggtcaagcca ctccctgttc tgtgccagga tgttcagggc
    21301 atctgcatcc agtaccagcg gtacggatat gttcttaagc agatccttaa gaacggaaac
    21361 tgtatcaggg tcagtgccca gtccggggcc ggcaccaatg gcatcatacg gtcccaggtc
    21421 aggcaatacc gtcacgtttc ccgttccgct gtcaggcgat accatggctt cagggagggc
    21481 cgcctggaga gccagcactg ctgaggatgg agtgtgaaca gtaaccagcc ctgcaccggt
    21541 gcgaagtgct gccccggctg agagcgtggc ggctcctgcc ttgccatagc tgccggcaat
    21601 cagcagaacg tgaccaaaaa cccccttgtg atcaaacttg tttctctcct tgatcatcgg
    21661 cctgacggtt tcgcgcagga cataatgata aggagtgttc atcgacgata tggtatcagc
    21721 atgaagcccg atgtccgtca cctcccattc acccgtgtaa tcactgtttc cggcgaacat
    21781 gaaagagagc ttcgggaact ggaaagtgag cgtttttgag gccctgacaa tactgtccct
    21841 gtctgaagcc tgattatcct cgcagaaaag tcccgagggg acatcaaccg aaattacaaa
    21901 tgcacctgtt tcgtttatgc gtcgtatcac tcctgcagca acccctgaag gggatttacg
    21961 cagaccggtg ccaaagattg catctatgat cacctcgtca cgcccgattg ccggcacctg
    22021 tccgggatcg gcaatggtta agggaattac gccgctgcgc tccaggcgtt cggcattcag
    22081 actgaactca ctgctgcggg ctgcgcctgt ctcaacaaca aataccctta ctgcgtagcc
    22141 gtcctcatgc atcagcctgg ccaggacaag tccgtcgccg ccgttgttac caggccctgc
    22201 aaatacattg atcttccgct ccggggccac aatgtccttt acctgctgga aaagagtcgt
    22261 ggcagcacgc tccataagtc ccgcaggagt gacaggctca agtctgacgg tggcagcatc
    22321 tatttcccgg atttccgctg atgtgaatat cttcatggtg taccacttgc tgatgtggtt
    22381 acaaatgtaa aaatatagac aatgcaaaaa tatcttcctt gtgatttaat tcaatcactg
    22441 ttatttgaaa agattaatat atttgtcacc gtcagaaaga actaaaccaa ctaatacaga
    22501 aaactatgct caagaatcat ccaaaagggc tacttgttgc cttcttcgcg aacatgggtg
    22561 agcggttcgg cttttacacc atgatggcga ttcttgtgct tttcctgcag gccaaatacg
    22621 gtctggcagc ggaaaaggca ggagacattt acagctggtt ctattttgcc atctatgcgc
    22681 ttgccctggt gggcggtata ctggcagact caacacggaa atacaagacg gtgattctgt
    22741 tcggaatcat catcatgttt gcggggtatt tactgatggc tgttcccggc atgccgctca
    22801 cattcacggt cattgccctc ttcaccatcg ccttcggtaa cggcctcttc aagggaaacc
    22861 tccaggctgt tgtgggccag atgtatgaca acccgcagta ttcaaagctg agggacacgg
    22921 cattcatgat tttctacatg ggtatcaacg taggcgcctt cttcgcgccg tttgccgcaa
    22981 acggcgtcag aaactggtgg cttaagacca acggatttgc tcatgacggc agccttcctg
    23041 cactgtgtca ccagtttgcc ggcggccaga ttaccgaccc cgcaaagctg caggagttcc
    23101 agaccctcgc tgacaaggta aaccttgccg gacccgtctc tgacctcgga gcattcgcag
    23161 acagctatct cgatgtcttt tcgagaggtt acaactatgc tttcggaatt gcagcagtag
    23221 caatggtgat atctctgctc gtctatataa ccttcaacaa gcttcttccc aacaaggaaa
    23281 aacctgtcaa ggcatcggct gactctgcca acacccttga gtttgaagtc aggcccctta
    23341 tttatgctgc cattgcgatg gcgatcttcg catttgggct tcagttcctc gtaggatggg
    23401 cgtcaggtct tgctttcggg ctgttcgcag gattcgtaac ttggatcctg acaacggcta
    23461 agaaggacga atatgcccgt gtctcttccc ttatccttgt attcgtggtg gtgatctttt
    23521 tctggatgtc attccaccag aacggtctta cgctgacact atttgcaagg gactacaccg
    23581 cgaagcaggt gggaccattt acgaacctct tctttaccct gccctcaatg cttgccgtta
    23641 taggctcaat cgccggcctg gtgatgctgg tcaggaagaa catgacaggc accgcaagga
    23701 ttatcggagg cgccctgttt gtgtgcgcaa tggttgccgc atatcttttt gtgacaaagg
    23761 cagatgccct gaattcaatc gagcctgagg tgttccagtc attcaatccg ctgttcatcg
    23821 tggcccttac cttccctgta atgggtgtct tcgcatggct taacaagaaa aacattgagc
    23881 cttcaacacc caaaaagata ggtatcggta tgcttatcgc agccgtggga ttcgtggttg
    23941 tcctcctttc gtcagtcaaa ctgccgtcac cggcatcact tgaaggatct ccagttgccg
    24001 aggctctcag ggtaacccct tactggctca tcagcagcta ccttatcctg accgtggcag
    24061 aactgttcct gagtcccatg ggcctctcgt tcgtatcaaa ggtggctccg tcgagattcc
    24121 agggactcat gcaggcaggc tggctccttg caactgctgt cgggaataag ctcctcttcg
    24181 tgggaacgaa aatgtgggac aaggttgagc tgtggcagct ctggaccgtg ttcatcgtct
    24241 gttgcctgct gtcagcagca ttcatattct caataatgaa gagactggaa aacgctacaa
    24301 aatgacggat cgtcagtaac cataagggag agccgcaaac acggctctct tttttttgcc
    24361 aattaatgat tcatgtctgc atgaaaaaaa gatataaata atatatttgt caggttaacc
    24421 tgatcacaat gaacagcagc aaaatgtatg acaaggtctt cgcgatcttc ggaacagtaa
    24481 tggtcttctt ttatttcgga ctggctatct tcgtccttac cacgaagata ttcgacatag
    24541 ataaagccat gaggatcata ctggccgtcc ctctattcat ctatgccatc tacagggttt
    24601 ttatatccta tgaaaagatc agggacagtt ttttcagcaa taaggatggt gatgaataat
    24661 ttaaagaaaa agagagggta acagcattac aaaacggtca tattctattg gtggcacaga
    24721 ctttgaaaac attaattgag gaagaggtga ttttttttaa tatcacggat cccgatttat
    24781 aagcatattt ttattaatat tgtggctcat agcaagttga taagcaaagg aacgatgaat
    24841 aaagcaaaat ccctcacggc agccgttgcc ggagtgatct tcctggcatt gctgaacctg
    24901 atgccgggct gcaagaacaa agggactaca gccttaaagg agaccccgac atcaggcact
    24961 atcagaattg catctgatga atccttcaga cctataatag aggcggaact tgataccttc
    25021 accgctctct actcatatgc ccacatcacc ccgctataca tgccggagaa tgaactggtg
    25081 gccttcttcc tgaatgattc ggtgaaggtg atagtctcat cctgggcacc tacggaagaa
    25141 cagaggaagc tgcttcttga catgcagacc gtggttcgta caaccgttgt ggcatatgac
    25201 gccctggcta tagtcctcca ccgcagcaat cctgattcac tgctgaccta cgagaacgta
    25261 aaggatatct tcaccggcgc agttactgac tggaaacaga tcaacccttc atcaaaactg
    25321 ggtcagacaa acgttgtgtt tgataacgtc aggtccggca acataagata tttcagggaa
    25381 aaattcaccc ttcccgaaga attgctgcca aacttcttcg cagtaaaatc gaatcctgag
    25441 gtaattgatt acgtatcacg tacaccggga gcaataggca tcgtcagtgt caactggata
    25501 agtgacgagg atgacccgca gagctttggc atgctgggca agataaaggt tgcggccatt
    25561 tcacaaccct acctcgataa aacatcatac tatccgcctt tgcagggaca tatttacgaa
    25621 aaaacgtatc cgtttgtgcg tgaggtaaat atgcactcaa gggaatcata tacaggactt
    25681 ggctcgggat ttgtatcttt tgttgcagga gaaaaaggac aaaggataat acttaaatcg
    25741 ggtcttgtac ctgcaacgat gccgattaga attatacagg taaaaaatga gtaactaaac
    25801 ttgataacga tgattatgag aagctttaaa ctcgcgttgc tgcccgcagc ggtattttgc
    25861 atggtctttg gcacggggct caaggctcag accctcgatg aggcaaggac tttcaccaag
    25921 aacgaacagt atgataaggc agaggttctg ttccagcaac tcattaaaaa tgagcctaca
    25981 aacagcaaga tcttcttcca ttacggggaa aacgcactgc tgaactactt tgctgacact
    26041 atctccaact cgctgaatat tgcgatgaag gaagctgccg acatcttcga caagggtgtt
    26101 gcagccaacg ctgctgaccc gctgaaccat atagggcttg caaaggttgc cctgtacaac
    26161 ggccagaacg ccagggcaga ggaactgagg ctgaaagcca agggactcct tcctccctac
    26221 aagaaggtca ccaagatcaa agatccgaaa ggttacgcct atgcccttgc caagattgcc
    26281 gaatcataca tccggtttga gcaggttgac accagcaagg cactcccatt cgtcaggcag
    26341 gcactctcaa ttgatccgag aaacagcgag gtatatatca ttgctggcga catctatgac
    26401 ctggtaaatg acggctcaaa ggctatcaag aactataacc ttgctcagga ctgggatctg
    26461 aaatcccctg cagcaaacat gaaaatcgga agtatctatg ttaagggacg caacctcact
    26521 gcagccatcc cctactatga gcaggccata gctctcgacc agaactatgc acccgcgtac
    26581 agggagctgg gacagcttta ctccatggcc ggaaggttca atgactccaa aaagtacttt
    26641 gagaaatacc tcgaactgac ccagggaaac atccccgcca agatcaggta tgtcaacgcc
    26701 ctgttctatg ccaaggaata tgacgaggtc atcaagaatg ttgaggaaat atttgccgtt
    26761 gaccagtcaa ggacatatat gaaccgtatt gccggttact cagcatatga agagggcaat
    26821 ttcgagcttg caaagcagta tatggacaag ctttttgcca accttgaggc tgaccgcatc
    26881 atcaagaagg actatatcta ctatgcaagg attcttgcca ggaagaacca gaattattcg
    26941 aagcttatga tagacgtcga caatgatgct gacgaactta gtaagctgca ggcaaggtat
    27001 gttacactga agagccctgc caaagagcag gcaaaggcat caattgacgc actgcaggcc
    27061 agggttgaag caggccgcac acaggtcaaa aaagccgaag ccgagtttgc aaaggcattc
    27121 gaagcatatg acaaggcaat cagattcaat gaagaggaag acctgaacct gatgtatgaa
    27181 aagggaacca ttcagtacgg tgcccatatg tatgccgaag cagcatcaac ctggtcacag
    27241 ctccttgaga agggaagaga cagtgaaaac gatttcctgc aggtgggaag ggctttttac
    27301 caggataaag cctttgacaa ggctgaccag gcctttaaca agatgattgc gaaataccct
    27361 gacaacctgc agggttacct ctggctggcc aacattgcct ctgcgaagga tcccgattct
    27421 gaactcggcc ttgcaaaacc gaggtttcag atgctcctca acaaagctgc cgctgactcg
    27481 gtaaagaacg tgaaggaaat atatgacgcc ctccgttatc ttggctatga agccctgcag
    27541 aacaagaggt acgacgaagc aaaggcttac tacaacagga tgatgaacct ctcacccgaa
    27601 tatcgcatca gggcccatag ctcgctgagc accatgtata tgaccatggg tgactatccg
    27661 aaggcaatcg aggaaaacaa caggatcctt gccattgaac ccggaaacga tggcgcaaag
    27721 tcggccatcc agtacatagc ccagcttcag aagagcgcac agcctaaagc acatcccaac
    27781 gaaatcacag gtgtgatctc tgattccaac ggcgatccca tcccgggagc ctcagtaagg
    27841 gttaaggaca ctgctgccga agcctggacc aatgcaaggg gtgaatacaa gtttgtgatg
    27901 ccggaagcat cagagacact tgttatcagc gccaagggct acaagtcgat tgaaatacct
    27961 gtttcaaaga gaaggactta caatgccaca ctcgacaaat gacgcttaat tgttaatgag
    28021 ttataaaatt gatctatttg attttggcta ttgtaaaaca aattgataat aattattttt
    28081 gacacctgtt tacttaaacc aactaaagaa tactaaaatg agtaaaaaaa ccaagtcgtc
    28141 aagtggtgta tggcagtcgc tcttcgcacc cctagcaatc attcttgcat ttattgttgc
    28201 ttaccttatc ttcacccgcg ttctgggtaa cccggttaac tttgaaggtg gcaaccccaa
    28261 gggtcatccc ctgcagggta acctgctcgg tctgatgtac aagggcggtg tcatcgtgcc
    28321 tgttctcatc acaattgtga taacgcttat catttatttc tttgaaaggc tcatcactct
    28381 gacaagggta aaaggaaggg caagacttaa cgtgtttgtc tcaaacgtac agcagatgct
    28441 cgcagaagac aggattgagg atgcaatcgt tgcatgtgac aagcagaagg gttcacttgc
    28501 caatgttctc aaggcaggtc tctcacgcta ccgtgccctg gaaaatgaca aggaactgga
    28561 aaaagaccag aagatcactt caatgaagca ggctattgaa gaggctacag ctctcgagct
    28621 ccctgttctt tcaaggaaca tggtcatcct ctcaacgatt gcttcgatct cagtgcttat
    28681 cggtcttatc ggaacggtta tcgggatgat ccgtgcattc gccgccctgg cacagtcagg
    28741 tgctcctgat gccctcgccc tctcaaccgg tatttccgaa gctcttgtca atacagcatt
    28801 cggtattaca ggctcaaccc tggctatcat cttcttcaac tggttctcat caatgattga
    28861 cagctatgtg tttaaaattg atgaggctgg cttcagtctg acccagacct tcgccgcctc
    28921 tatcagaaaa taacttccga atttattctt ttggtcaaaa aagaaaggaa ttaaaagatg
    28981 ccaaagatta aattaggaca caaatcgcca cgaatcgaca tgacgccgat ggtggacctg
    29041 ttcacactgc tgctcacatt cttcattctg acagcaacgt tcaggcccca ggaatcggct
    29101 cctgttgata cacctttttc cgtatcggag aaacccatga tggatgccaa cgtaatgacg
    29161 gtactcatcg cgaaggacaa ccgggtattt ttcaatgttg acaacggacc ggacaccatt
    29221 ctgaaattcc ggccgaaaat ccttgccgaa atgggcaagc gttacaacat tgaattcacc
    29281 gaggatgaac ttcgcctctt cgagaagacc ggcactgccg tgggcgttcc gatacaggat
    29341 atgaagaaat acctcgcctc gaaggattcg gatgagaaaa ttgcactcga aaaaggtgtg
    29401 cctattgact caaccgataa ccagcttgcc ttgtggattc tctttgcacg ccagatcaac
    29461 cctgcagctg atgcaatgat caagggcgac gcagacgttg aattcccggt tgtaagggaa
    29521 gtacttgata ttctgcagga caagaatgtg aacaggttca acctgatcac gaacctgagg
    29581 tcagctgaag taaccgaaga acaaatagca gaataagatg gcagacgtac aagtccagga
    29641 acaaagcaaa ggcgggaaaa agaaaccaaa aaaaggtgat gcccgcgtgg atatgacgcc
    29701 gatggtagac ctcatgagtc tgctcatcac gttcttcatg ctcacagcag ctttcagcaa
    29761 acccaaggtg atggaaataa tccttcctga aaaaatcaag aagaatgaaa aagtagaacc
    29821 accgaagatt gctgacagcc gaactctcaa tatcatcctc ggtccggaag acaaagtcta
    29881 ctggtacccc ggcaaagcag accctgaagt gacagtactc caggaaactg atttcagcgc
    29941 aacaggaatc cgtcaggtac tccttgaccg gaacagggcc ctgttccgca aaatagacca
    30001 gtttgagaaa gatgttatcg caggtaagat caatatcaaa caggactcca tgagatcagc
    30061 catcagccag ctgaaaaagg atgatgatac agggcctatc gttctaatca aagcctacaa
    30121 gacagccaag tacaaaaact tcgtcgatat catggacgag atgtcaattg tgggtattgc
    30181 ccgatacatt tttactgaca tagactggat tgaggagaaa ctggttgtcg atgccatcaa
    30241 cagggcagca gccactcctt caggttcagc acaataggga gggatacaac tatggcaaac
    30301 gaaaagaaac gatacccaag cttcgatgat atagtattcg aaggcagaaa caaggaatac
    30361 ggcgcatacg aaatccgcag gaaatatgcc aggacaatga caatgtcaat tatcattggc
    30421 atcatcgtgg tcttctttgc agcctacatt ccttatatca aggccaagag tgttgcacag
    30481 atcaagcaga gggacgccaa tgaggtcata gccgaaatgg ccaatgacat caaccaggaa
    30541 gccgctgctc ctcctccgcc tccgccaccc ccacccccgt cagaacagca gacagtggtg
    30601 aaatatgtgg cccctgtaat cgttgatacc atcaaacccg aagacgcatc ccagtttatg
    30661 actgctgacg acgtggccga gaccgtggtt gacgaggaag tgggacttga ggtaatcgaa
    30721 caggtacagg aagaggtaca ggaagaagcc ccgcgcgaag tgtttgtggt ggtagaagag
    30781 atgccttcat tcccgggagg ggataccgag ctcttcaagt tcatctatga caacatcaag
    30841 tatcctgaac ttgccaagga aaacaacatc cagggtaagg tgatacttcg cttctgcgtc
    30901 acatacaagg gcaccgttga ccaggtatcc gtggtcaggg gcgttgaccc gtctctcgat
    30961 gaagaggcaa tcagggtcat caagatgctc ccgctgtgga aacccggtaa acagggcggt
    31021 aaacccgtga acgtgtggta cagcgtaccc atcagcttcc agctcaagta acggaaactg
    31081 aacggatata aaaaaggtgt ctcaattgag gcaccttttt ttatgctgat tcaaatgcac
    31141 agttcagcgg ccgccaagca tcaacgtgat gtaacgctca gccagcggcg tcctctcaaa
    31201 tggcaggccg ctgaccagta tcagctttcc cccctgcctc gccatatgca gtaccccgtt
    31261 gaacccgtcc tcaaaggcat acttgaaaac ctcctcatcg gctgaccatg catctccggc
    31321 aagggcggct cccattgcgc cagcatcagc cggatcttca cagctgaaga gatatatgtc
    31381 aaaccggtct ccctcaacct cgtacgaagc cctgaaggca ccacgaagaa actcgtgccc
    31441 caggacactc tcaagcaggt aggtttcctg gttcttcaga agcccttcgg aaggaaagag
    31501 cccgagcagt gccggaaact cccccgatcc tccaataccg gacgaaataa gacccgcaag
    31561 atggagcatg gcgctgttca cttttgcaga gcgggaatgg ctcataacct tgacataaaa
    31621 acagtctttg tagaaatgaa ccgcaccttc ctcattgtag ccctgcaccc ctgtgttaat
    31681 gaatttatat cccggcgagc gctccatgga gtaaataccg aaagccctgg catcatcacc
    31741 aaaccggtag atctcagcct tgatgctgtt cctgcccttc acatactccc ttatgttcag
    31801 gtcaaggaag ccaagctgaa ggtaggcgtc ggctgcacca ttgatatagt tccacaggtc
    31861 gtcaggagtg taaacgggat agtcattgct catcctgtac cccttcagat ccgggaaaag
    31921 atcctgaccc tgaatagcct gaaatgaggc aatgagcagt acagaaataa aaagatttct
    31981 tttcatcgcg ttgttatttt atcccaggag gtcatacggt acattcgcca gcctggatat
    32041 gtcgctgatc ttacccctga tatcaaatcc ccttacgcag ctggcagtgc atgtgtcaca
    32101 gccggcacag ggattggcct caaccgcagt ctcgctgaca agctctgagg ccatagcggc
    32161 attatgatag ccgtaggcat acatataagc cctcatcagc tccggtatcg gcagattatt
    32221 ggggcagccg gggacacagt tgctgcatgc attgcagtaa agcccgggta atgaggcttc
    32281 agccaccagg tcagcctttt cctgatctgt gagcactatg tccttcataa ttttcagatc
    32341 tgtctccagc tggtcaaacg cagtgattcc gggaatggtg gtatgcacgt tcggatttga
    32401 caatacccac ttcagggcag cctgtacgtt gacaggcttg gttctctccc tgtcaagcca
    32461 tcctccggcc aatgttttca tggctaccac gccaatgccc gctgcggcag ccttctcaat
    32521 ggcgctgttc atctcagcaa ggtaggtctg gcggtagttg tatgatgtga gcacaacatc
    32581 ccatatacca tcttcaacca tggtattgat aaccagcggc tcattcttgt gtgttgatat
    32641 cccaatgaac cgaacccttc cctgcttctt cagatcaagc atcgctttga cagtctccct
    32701 gtgcctgacc atctcaacac tgtctgcaga gtgcatgtaa agaatatcaa catagtcgag
    32761 tttcagcctg ctcaggctga gattaaacat tgccaggaag ctctcggccg tggtatcctt
    32821 tgagggaagc ccggtacggg agtcaacccc cgccggcttc acctttgtgg ctaacacaaa
    32881 tgattcgcgg ggcagagtgc tgaaaaatcc tccgagcatc tcctcgttcc ggccgttcat
    32941 atagccgtga gccgtgtcaa aaagctttat tccaccctca tgggcagccc ttaccagaga
    33001 actgttgtca gacctcatca cgccgaagct gactacgggc agcctgagat tggttttacc
    33061 cagggtcctg tatataatct ctccctcatc tgttttaccg tttacggcca gggcccggtt
    33121 acccatcaat gtgatccctg ctgctcccgc aaggctggat ctgaaaaagt ttctgcgttt
    33181 cattgggaat ttttggtcgt cttctatgat ttctgcaaat atgctcactt cagggcagtg
    33241 ttgataaatt cattctgttc ttatcaccgt gccgttgaga atgagccaat atacaaatat
    33301 tttatcttat tttagcgagg ctgtcgcgca gcaaaaaatc aggcaatcat gggacgatat
    33361 atccacaacc tgatatcagg cggcgagggt ttgaaccttg acttcaagta ctgcatctct
    33421 gacccgcaga agattgcgcg taccctgtca gcattttcaa acactgccgg gggaaagctg
    33481 cttatcggag tcagggacaa cggatcactc gccggcgtaa ggtcagacga ggagtattac
    33541 atgatagacg ccgccgccag gctccattgt gaccccgaag tgactatccg tacaagaaac
    33601 cacactgtta acggcaaaaa catccttgag gtagaggtgc cgaaaagcaa cataataccg
    33661 gtaaaggcca gggatgaaca tggaaggtgg aaagcctatt tcaggcaaaa cgaccagaac
    33721 tttatggctg acagggtgat ccttcaggtg tggcggcgca gcgtaaagtc acgcggactg
    33781 ctcctgaggt ttgaggagac tgaaaacctg cttctgaatc atctgcgaag cgagggcagg
    33841 ataacggtgg aagaattccg gaaccttaca ggtatcaatg ccaggagggc cgaaaagata
    33901 ctcagtgatt ttatcctctg cggcctgatc acctacgagg ccagtgaaag gggaatcttt
    33961 tacaggctca gcactgagtt tggttcaggc aggtgagcaa tcagtcaatt cttaatcctg
    34021 ctaccaatat atcatcagtc tgctgcgttt ttcccatcca gctgccgaga cgctcttcaa
    34081 gtatctcctt ttgtttgctc atcgggtaat tctgtatgtc aagaatcagc ttccgcatgt
    34141 ttctcctcat gaatttctta cctgtgacgc cattgaactg gtcagggtaa ccgtctgtaa
    34201 agagataata tgacgagtcc ttctccagtt tccatgtgtg ctgtgtaaac acgtgatcac
    34261 cgtcaagatg cattcccagc ggcaccctgt cgccgtaaac ggtttccagc agatacttgc
    34321 cgctcacctg tgtccctgca tttggtttga attcgccgtt gtcccatctg gccttctcct
    34381 ggtcactcat ctccctgacc ctgaagcatt gcatggctgc acccgaatac tcaacagtct
    34441 tcctgcccct gtcaaccgag aggagggata tgtcaatgcc ctcatgctgc acttcaccgg
    34501 caggaagggt tgagaaagcc ctggtcagcc tgtacctgaa ttccctcagt atttccgagg
    34561 tgctcatata ccctggtctg tcagctatct catcaaggaa gcaaagtgcc attaccgtac
    34621 gaagtgcaga gtgcaccccg tgacccgtac agtcacctgc agctattatt gacctctcac
    34681 cctgcctggc tatccagaag aagtctccgc tgacagtacc cctaggtctg ttaaggagaa
    34741 aactgttcgg gatggcttct ccaagccgcc tctccgaagg cagcagagtc tgctgaacca
    34801 ggccggcata tttctcaagc ccttcaatct cgttctttcc ccttgccgta gcttcattcc
    34861 tctgcctgag caggctgtca agcctcctgt tccttatgcg cagcctcctt gcaaagtacc
    34921 ttatcaggag gaacaggagc cagcctgcca cggctgcgta gaatagtctt gcaataagtg
    34981 aggtcagcca cggcttcctg acagagaatg aataggaaag ctcctctccc tcaaaccccg
    35041 ttattgttct tgccttgaca acaaaccggt aatcacccga tggcagattg gtgaaatccc
    35101 tgtagttacg tttttcccag gctgaccagt catcatcata accctccagc cggtgcctgt
    35161 actctgtctt ctcctcatcg atataagagg tagtggtcca gctgaaggta aggctgttgt
    35221 cactgttggg aagggtgata cctggtttat caggctgtat tggggacggt atccgttgcc
    35281 cctccttcgt gtcggtataa aaggtgccat ccatgagtac cagtccgctt ccggtaatta
    35341 tcctggtaaa cagcgtgctg aaatcaccgt acctgaacga aagcttcgac ttgtccatta
    35401 caaagagatt ctgccccttt gcaagccaga cgcttccgtc agcatacgat attcccgctg
    35461 ttgccagatc aggcaggaaa tcgaactgac gcctgaagac cacatgaccc tggcgggtgg
    35521 ttgtaaccat agcatcaaag ttgcgcgagt cacagcctga gatgacaatt tccctctccg
    35581 gtgtgctggt caccatcctt atgttggcat catcaaaagt ttctccggtc agattatgat
    35641 ccttcctgaa agtatcattc tgccggtcgt aaacacagat accccttcct gtgcagaggt
    35701 aaagactatc attgatgact gcaagactgt tcagggtgtc agatctcacc ccgctgtccc
    35761 tgccataaac tataaaagca gtgtcatttg actcacagtg cattcttacc agtgatgccg
    35821 gatctgatgt tgcgagccac cattcacccg gtgcggtctg ttcaatatca ctgatacggc
    35881 ctgtaaccgc gttgctgatg gtatgcctga cttcccattc ataaccatca taaatcagcg
    35941 ttctgattgt tccgtcgggg gagcctgata ccatcacccc gggattccgg actgagttgc
    36001 aggcagcagt aaggtgcagc ccgggaagaa aacgctcaag gtcgtcattc ctgtcaatct
    36061 gcaggagtcc gttttctgtt gcagccagca gcacacttcc ttcccggagt tccaccacgg
    36121 ccagtgaata aaccctggct ccctggctcc cttttctcct gaaccgcctc actcctgatg
    36181 ggtcggtata gctgacaaga agaccatctt cagctccggc atatatcgcc ccggcgtatt
    36241 cagcaaccgt accgcagaca gaggttatac ctcctgcagg ggtgaatccg gatgccggca
    36301 ttgatatgga tgccctgttt ataaagccac tggtgcagaa ccacagctgt gaattggacg
    36361 tgttattgca atacatggct gtcactgaag actcagccac tgccgccgtt ctttctgata
    36421 tgtgctggag gagcctcccc tcatgactat agatatagac ccctcccctg tctgaataac
    36481 cggcagcaat caggttgccg gggagcagga caacatcggt gagtgaaccg cccggcaccg
    36541 tggtctttga ttccgtaaca accggaagac gagcgatagt gcctgttctg atgttgaaca
    36601 gcatcagtcc cttttgtgca tcgccgatga gaaggttgtc cctgtcatat ggaagaagcc
    36661 tgacaaacct ggcaggggcc gctttttccc ctcccgggat tctttcggtt ctgtcagcgc
    36721 tgaatgcaaa cagtccctcc tggttgtcag caattattat cctgttgtca aatgcaagca
    36781 atgcagagac atttcgtatc cccaattcat tttccaggtc ggtaactgta actgaatctg
    36841 atttatgacc tgagacaaac agtcttctgc catcactgaa acagacatga ttgctgtcag
    36901 ccgcacctga ggtaatggga ctgtagttcc cctccccgct tctggtaagt cctgcgagtg
    36961 aaatataggt cagtctgcct gcattgtcag gctgcaggaa accgaacccg tttcttcctc
    37021 cggcatagac aattccgcga gtgtcagtga tcagtgaggt aactgctccg atacccgggg
    37081 tggctatcct gctccacctg ctgccgtcat atgtgacaat tccgccggat tcattgccaa
    37141 agtacattac cccgcggctg tccatagtga tacacaggtt gcggagctct cccggagtag
    37201 cggatgcatc aaaccggctg atcaaagggg tgccatacct gttgacctgc gaaaatactg
    37261 acaatgatga taatagaagg agtatcgtaa ccttgaggcc cttcttcatt ccctgaacaa
    37321 tttccctgaa tttatggtta aatatacgaa ttcaattcat ataacggctg aaaccacttc
    37381 cggcatataa attgaggtaa tttttagtta tattggcttc gtatattcag gctatgaggg
    37441 taaaacagag ggcaaccgca gtgttggcgg ccattatgct gctgcttaac ctgcccgatg
    37501 ctgattccca ggcttatgcc ggaaggacgg ccggtgacaa acctggcgta aaagtgtcgc
    37561 gtcagctgtc tgatttcatt taccgctcag gtgaatcaga atttcagacc atcaccgacc
    37621 cttcaggcaa gtacctaagg ctgtttcttc ccgggcacca tcagtctccc gagaccggac
    37681 agcccgagct gcccgtgtgg agccggctgg ttgaagtacc ggatggaatg gaggtggttg
    37741 tctccatctc ggaagtgagt tcacgcagaa tccggttcgc tgaccgggga gcccctcagg
    37801 ccgagctttt cccggcacag ccggcaagga ccaagaatca ggtacgtgat gacaggataa
    37861 ccgtaaagga ccgaaaagcc tatggcagcc ggaacaccct gatgcatgac accgtaaggg
    37921 tgactcatga aggcatattc aggggccgcc gtctggcaaa catcgcagtc tatccggctt
    37981 tctacaatcc agggggcaga tacgttgatc ttatcacgtc aatgaaggtg gagatcgggt
    38041 tcagaccgga agcaacaaag ggtgaagagg atacggagga tccggtcaac aagggcggat
    38101 ttgcttcaga ctcctatatt acaggctaca ctgaccggcc ggttcacatg atcatagtca
    38161 ccgactccat cttccggaaa catcttgagc cactggtcag atggaaaaca ctgaagggaa
    38221 tacgtacaac tgtaatttac aggaaatcag ggcctgctga cacagtatac aaggatctga
    38281 aaaacaggat atcaaccgtt tatttcagtc ttcaggaaaa ggggatcccg gtacattacc
    38341 tgatgatcgc cggcgatcag tccataatac ccacatcacg cggtaccacc aatgtgtcag
    38401 acctttacta cggcgagttt gacggccagg gcgactatat ccccgaactg ttcattggca
    38461 gacttccggc atcagatacc acccagatga aggggatggt caaaaagatt atcgattacg
    38521 agacaaacag gtttggtccc gctattgaca actggtcacg ggcactggcc accgctggaa
    38581 atgcgccggg atttgagctc tatatgaacg gacaggtgtc atacatctac aacacttacc
    38641 tgaaagcaga caaatccctg aatgccatcg gctggctgta tcccgacgct cctcagaagg
    38701 atgactcgct gaagaccgtt ttcaacaagg gagtcagcat acttaattac accggtcacg
    38761 gtgaagccac cggcttcagc gaccctgctt tcagaacctc aatgatgaac gagttcagca
    38821 atgaaaacaa gtaccctctc atcatagcca atgcctgccg tacagcccag ataaacgttg
    38881 catcgtgctt tggcacagcc atggttgcca ccccgaacaa gggggccatc ggttatattg
    38941 gatgtacaaa tgactcttac tggagcgatg atttcttctg gtcagtgggt ccgggaacac
    39001 ccgggccgaa tgtcaccttc gagactaccg gtgcaggcgc ctttgacagg ctgttccata
    39061 cacacggtga gcctccgggc gaatggtact acaccatggg tcaggtaaac ttttccggaa
    39121 acatgtccgt atcggccagt accagtccgc gaaagaagta ttactgggag acgtacatgc
    39181 tgctgggtga tccttccttg tctccggtaa taggaaggcc tgacactttc aggattgacc
    39241 ttccggacag ggtgccccag caactgcagg tccttagttt ctttaccggg ccttttgcat
    39301 atgcagcact gtcagatttc gacacgcttt gggatgccag gttcgtcagt ccttccggga
    39361 acatctctct ggccgtcccg gcaggcgtca aggactcatg cctcctggtg gtcacaggac
    39421 agaatatgct tcctttctac aagaccatcc atttcggtga tgtgcctgaa ccattcataa
    39481 ccgtcggcga catagtattc gatgacgccg atggcaatgg aaacggcctc cccgactaca
    39541 gtgaaccggt gaatctgcag gttacagtga agaatatcgg aaagatgcgt acctcaaaga
    39601 tgacagcccg gctaagcgtc gcctccggga tgataaccgt ggaagctgat actgccacga
    39661 taggggtgtt gctcccggga cagacacgca caataaccgg aaagttcatc ttcagggttt
    39721 cagacagtgt ggaggacggc gagctggctg ccctgctgct cagcatcaag gatggcagcg
    39781 aagagtacag gtacggcatt gatatgactc ttaatgcccc ggaactgaag attatttcag
    39841 ccattcatga cgattcctcg ctgggaaatt ccaatttcct tcccgatccg ggggaaaaac
    39901 tgcatctccg ggtcagggta aaaaatgacg gatcagctgc tgccaccggg ttggtgaaga
    39961 taacccctgc cggaccaatg cttaccgtat accagcccga gctggctact gacagcatca
    40021 aaccagggca ggtgaaggat atttattttg agacagaaat ctcacccctt gccggatccg
    40081 ggaaggttat cccctatgac gtaagcttca tttgcggcaa atatgaggca aggggcaggt
    40141 ggtcagtcag tacggggaaa acacgcgaaa cctgggagtt cgacaggttc gacgtgttcc
    40201 cgtggatact taatggcgaa tacccgtgga taataacatc ctcggtatcg tatgagaacg
    40261 ttttttcagc acggtcagcc cctatacctg acaaaacgga atcgatcctt gccatatatg
    40321 tcaacaatcc ggtagccgac accatgtcat tttttgtacg ggtctcctcc gaacccatat
    40381 atgacgagct taccttcagg gtagacagcg tcactgacat gcagatctcg ggtgacacac
    40441 cctgggcaca aagaaagaaa gtgctgaagc cgggcgtaca cctgctggag tggatatatt
    40501 caaaggatgt ctcactctcg ggcggacttg acggagcatg gctcgaccag gttacattcc
    40561 ccgacatttc gttccttgag gcagacctgc atattgattc ggtctttgct cccccagcag
    40621 aggcaatgct ttcggaggtg accgtgaaag ggagggtgat caaccttgga cgcaacgcac
    40681 tgacaagctt cccgctggca tacagtatca atgaagggga attggtcaat gaaaccttct
    40741 acaggaaaat agacccgggt gacactgttg atgtggcttt cacacagaaa tgcagtctgc
    40801 tgaaggatat accatatcat attcatattt tcagccggct gcctgaagac ggttatgcag
    40861 gaaatgatac cgcattcgtt tcgtttgtca tatccggaac cggaccggaa atcaatgagg
    40921 atcaggtaat cctgcatccc aatcctttca atgaggattt ctcgcttgaa gttgacttcg
    40981 ggggaagtga gacggcaggc tttgaactgt ttgatgcttc gggaaggatc gtcatgcagt
    41041 cagaggctga gctgtcgccc ggcaggaacc ggattccgtt caattgccgg caccttggat
    41101 ctggtatcta tacccttcgg atcacatacg ggggcaggag cttctcgatg aaggcagtta
    41161 aaaagtgact gatcagacgg atcataagtg caattattca aatttaaaat caggaaatga
    41221 caggcaaaac acccaccagg gaagaagctg ttgagctttt caggaggtac aacaggtcag
    41281 acagcctcta caaacacgcc ctatcagttg aggcggtcat gagatacatg gcgcgcaaac
    41341 atggtgagga tgaggaaaag tggggaatca tcggactggt gcatgatctc gattatgaga
    41401 tgtatcctga ccagcactgt accatgacgg agaagatact ta
//
LOCUS       DAOPXU010000009        41219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_817_length_41219_cov_13.445632, whole genome shotgun sequence.
ACCESSION   DAOPXU010000009 DAOPXU010000000
VERSION     DAOPXU010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 41219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41219
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_817_length_41219_cov_13.445632"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..2827)
                     /locus_tag="PKK03_01705"
     CDS             complement(101..2827)
                     /locus_tag="PKK03_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNX83136.1"
                     /translation="MKRILLLSVLAVIQFLPVTAQFLGDGSMGNPYRGTLAGAFTISG
                     TKYFTGTIIIDNEQLTIAPGTILIAATGTSGILVNGTGQLNAQGTSSSRILITSNSDL
                     DGTYGEPTDTWGNITLISSGVSNISYCTIENGRRLATRIGYYGGGLYLGTSSVTVTST
                     TIRNCIAGYGGAIIVAGTTSPVISGCIITGNSAIEQGGAIYVAGGSSPVITNTIIYSN
                     SSLSTTRSGGSVASIASSPKIINTTIAYSSSPAAGGKSVYLENSPDARIINTIIWGSS
                     DNIGLSGTPSSVIEYCAIEGTSLAGCLTLNSSNTDPEGPNFTNPGSGDFTIAFISPCR
                     DSGTDSYPGVTVPLTDYAGGQRIGQTDIGAYEIRYSRWVGRTTDWTRPLGWDKGVRPG
                     TTDVIIPAGVATYPTVAPGPSFTLNAGLTMIIEPGARVTLSSLTNNGTLVLRADANAY
                     ATLITGSYSGAGGNANVDLFLTSGPTGEWWHYIASPATVSKTAFTDIEPELLVRYDES
                     KVLTDVSDGWQWHDGYGGTTPFSNLNAKEGYDVSVASDVIMTYRNLKSLTTSMGRIDL
                     PFSGSGGDTTVFGYSLLGNSLTCGINWDLVTYSHEHKLIRHAYYLRTASGEEASYVNG
                     VGTNGATAHIAPLQGFFVRTRATGTYITIPDNAREHTAAPRFKSAEQIPLIRLQFSSS
                     TQTDETVVRFDPEATTGFDGLLDASKPFSHVKGHIKIYSETGGENYSINAIPWPTQKT
                     SIPLTLGIHEAGTYKISGSQFQTFAGYTVVLTDRLTGSRTGLDGKAGYSFQAAEGTIS
                     GRFTLDIIPAEKKAIAAGEENKPEVIQETSLRIYSAPGRVCILPQGSGWDGTAGKVRI
                     FDITGRVVFMSDNERLYSGELKEFQASGNGGLLIVEVVTGQKRYLEKIVLAR"
     gene            3028..5217
                     /locus_tag="PKK03_01710"
     CDS             3028..5217
                     /locus_tag="PKK03_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX83137.1"
                     /translation="MQPERNTIRRALGLLAESSARETAISIIILIIRALLPLLAVLLL
                     RYFVDQVTGAAGSGMAEGTASEAFSAAPGMETVSGIAGSGTAEGTASEAFSAAPGMET
                     VSGIAGPGTGAGTTAEAAAGTGDLPLGGTGSLQLGGTGSLPRGGDGNLPWGGGMPAIL
                     IWLIAAMAVALLADDLLSYAGTYITRRHSFLVEGHISSLIHNHAGILGLKYFEDPLFH
                     DRLERAARDISWRPAALVSDLILLLRGVISFIAMAFVLRNFGLVPLLILVAVFIPFLL
                     VRARNSSKLWEARKKATADSRQASYFSWLLTGEKPAREVKLFDLGGYFDSLFRKHFRA
                     SKEPELEAVKRNILPESLASLLKVAAFAGVLIYATSRYLASSITAGELAMYLVAFRQA
                     LVYLRDTVTGYSGLNENRLFLNDLFSFLDLRPESEGGSKAPDPGSFREIAAEKVTFTY
                     PGGALPALQDVTVRIGRGEKVAIVGPNGSGKTTLVKLLCRLYEPDKGQVMVNGADASA
                     IEPGSYRKLFSVVFQNFMLYYLSAGDNIRLGDRWPSAIGGQQHYSRVKTGSGTNNCSD
                     EAGGAVTATGRKAEEEIQEPESMAEVRRAAATAGIGGLLEALPEGYDTMLGHHTEGGR
                     ELSWGEWQKIAIARAVYRKSPVLILDEPSSSLDADAEYEIFSDLSRITGGRTCIFISH
                     RLSIVRDADRIIVLDGGRIVETGTHEELMAAEGRYFTMFTRQKSMYR"
     gene            5214..6578
                     /locus_tag="PKK03_01715"
     CDS             5214..6578
                     /locus_tag="PKK03_01715"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNX83138.1"
                     /translation="MTGLEKEAPAEGNEGLQRAVSLLLLLSRMDFSSEQKRLIAEELK
                     SFNEWKLLAGLAVRHGVAALIWQNLSDLGHAGEVPATERTLLEGLRFKSIARVSWITE
                     AAAGVTALLEQEGIKVVLLKGLSLEHTVYGSKGLRQMSDADLLVAPSDALRARDILVR
                     EGFRSMSLKSPLYRHIILDLGNHLPELHRGGISVDLHYRLFGPEGEEMVERAIRDSII
                     TTGVDREPGRNVAVTRESEAVHDTEIGPGLARESVAGADTGSLASSRAGIPGTGTFRI
                     LPPMTAFLALVSHIYKHEIKGEFQLRLWTDIYLLLDRYGKQILISRLAAAAEEAGIRK
                     ETAAVLTVMEQVWGVALPESMAADSSAAQEAAVASFMNRLAHPGSVESISQREMFMRN
                     LKALKSPRKKFIFILGDIFPSVGFMKRRYNCRSKVAAFLFYPHRLGKIPWILGLLRTE
                     KYDN"
     gene            7121..8590
                     /gene="cysS"
                     /locus_tag="PKK03_01720"
     CDS             7121..8590
                     /gene="cysS"
                     /locus_tag="PKK03_01720"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNX83139.1"
                     /translation="MKSALYLYNTLTRKKELFEPLAPPFVGMYVCGPTVYSEAHLGHA
                     RPAVTFDVLYRYLMHLGYNVRYVRNITDVGHLENETDDTGEDKISRRARQEHLEPMEI
                     VQKYMNSFHHAMEKLNTLPPSIEPRATGHIIEQQQLTEKLIGEGYAYEVNGSVYFDVE
                     KYDGRHNYGILSGRVLEDMRANSRALEGQDEKRGQFDFALWKKAAPGHIMRWPSKWSD
                     GFPGWHLECSVMSTKYLGETFDIHGGGMDLIFPHHECEIAQSTAAQGHQPVRYWVHNN
                     MVTINGQKMARSLGNFITLEEMFSGSHKLLARAYSPMTIRFFMLQAQYRSTLDFSNEA
                     LLAASLGLQKLMNAEKTLAGLIPGGSSTADISGIAAKCHEAMSDDLNTPIAIAHLFDA
                     VRIINLVNDGKEKLSAEDIRSLQELFRIFIHDLFGLRAEESAGNDRLEGVLKLLLDIR
                     QEAKAKKEWAVSDRIRDRLSELGITVKDRKDGYDWEITA"
     gene            8587..10119
                     /gene="ispG"
                     /locus_tag="PKK03_01725"
     CDS             8587..10119
                     /gene="ispG"
                     /locus_tag="PKK03_01725"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HNX83140.1"
                     /translation="MSLTPYKSDIIQIGSLTMGGDLPVRVQSMTNTDTLNTASSVSQC
                     IRIIEAGGELVRLTAQGVREAENLAAIKSGLRAAGFETPVCADIHFNPGAAEVAARIV
                     EKVRINPGNYADKRASFIRQEFSDSEWKAELERTRERLMPLIKICTEYGTAVRIGVNH
                     GSLSDRIMTRYGNTPEGMAVSAIEFLKIFRGEGFNRIVVSMKSSDTLTMVMANRLLVK
                     MMFEEGMHYPIHLGITEAGEGEDGRIISAAGTGTLLAEGIGDTVRVSLSEPPEAEIPV
                     ARAIIKAVAGEAGRVKNPVAPLEKRRPGERWFPQVYTRERDRFNDESGAPYTGDVLTA
                     SPDELQATEGRQAYDRILNPVFNCDDPEQLAIEAAALLGRFFIARHPAGLCISNSGAV
                     QGDALRRLAFSVLQATEARITRTRYISCPTCGRTKFNLQEAVRKVKEATAHLTGMKIA
                     VMGCVVNGPGEMAGADYGYVGAGEGKVHIYRGTEAVLKNVPEAEALDKLLELINNDQA
                     RQ"
     gene            complement(10165..12309)
                     /gene="rnr"
                     /locus_tag="PKK03_01730"
     CDS             complement(10165..12309)
                     /gene="rnr"
                     /locus_tag="PKK03_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNX83141.1"
                     /translation="MSRKKPPEKSGIDQKKLIELIVKAMAKNPRQPLNYKQIAAKLKI
                     TEKEEKRALSEALLRMKKDGTADERGHGKYVLKAVGGYVTGTIDLTKFGYAFVITDEM
                     EDDVFIAARNLNTALHGDQVKVLLYARHKGKRPEGEVVEIINRSRATFVGSIELTKQF
                     AFLIPDNKNMPFDLFIPLAKLNGAKQGQKAVARIVEWDQRSRNPVGEIIEVLGNPGEN
                     DTEMHAILAEFGLPNRFDPEIEAAAEKIPAEITAEDISARRDFREIPTFTIDPEDAKD
                     FDDALSMRKLPNGNIEVGVHIADVTHYVRQGSPIEKVAQERATSVYLVDRTIPMLPER
                     LSNHICSLNPSEDKLTYSAVFEMDAKANVVNEWFGRTVISSDMRFSYGEAQKVIDTGE
                     GDMKEQILTLHNLAQLLRSKRFASGSFAFERLEVKFNLSEKGVPLGILFREFGTSNQL
                     IEEFMLLANKRVAEYVGRKLKGKTFVYRIHDKPDPEKLSSFSYFIKRFGYEIDTANVK
                     GLPAAMNKLMEEVSGKKEQNIVETLALRSMAKAVYSTDNIGHYGLAFGHYTHFTSPIR
                     RYPDMMVHRLLTKYLEGQPARDKDKYEKMCEHSSKMERLATDAERASIKYKQVEFMSD
                     KIGKVYEGVISGVTEWGIYVEIIENQCEGMVAIRELQDDYFEYDEENYCIRGRQSGKV
                     YMLGDKISVEVVKADLQKRQLDYRLAGSTEKE"
     gene            12370..12666
                     /locus_tag="PKK03_01735"
     CDS             12370..12666
                     /locus_tag="PKK03_01735"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HNX83142.1"
                     /translation="MIMISLSSIPSHSPSVVYRRINDEYLLIPLTGNIADMDSLYRLT
                     ETGAFIWELIDGERAISDITSKIVEEFDVEADIAEKDALDFFREIQEFLHFRET"
     gene            12663..14024
                     /locus_tag="PKK03_01740"
     CDS             12663..14024
                     /locus_tag="PKK03_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83143.1"
                     /translation="MTVDLVIAGRRIRFHSREGLDVRPDERFSAFTVAAEAEPDLIVD
                     VEPGSGEIPPAAMQVFDAQLMEEVPGGVINSGESFWEIFSGGGVTYARVFLKEPQRTA
                     VLEMPHGEMRWRIRTGEFAISPQLSGGKDQPAAQFTAGPPNTINPLPYPLDGLLLYFL
                     FSRAGDIMIHGSGVSCHGRGWLFTGRSGSGKTTLAQIFDRAGDRIIHDDRLVLRREEG
                     RWVMHNTPVYRNDEPRSAPLDHLWIIRHGSANVSEPVTGAEAVAMILANCIQQNWDRE
                     AAARLAAAADDLASSVRVSRLSFLPEGSIRDYLRLRKEEDISIAAGAAEALLNAGKII
                     TVTAGGYSMWPAIRPGDKVEIEPIGTRVPEIGEIVALKRDGGYVIHRVTGVTAGRNAT
                     LIRTQGDGVTREDEPADAGMIAGIVGSVVRSGRQRQPPTRHLPRWFNRMTARLQCWIS
                     PKV"
     gene            complement(14126..15670)
                     /locus_tag="PKK03_01745"
     CDS             complement(14126..15670)
                     /locus_tag="PKK03_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83144.1"
                     /translation="MRNFLQILAAILLVFSACTKPEPEPNIVVDENGLAENMFTEWPG
                     YDPVISYKFSDDYPDYEMPVRNLPYTGRNLSWTLADGWWSFYAGSKANPLVTEEAVQP
                     MLDRLNTDFAYMRDVMGWPPDMAVQNGYRSAVFLYGSGLSTDDASNTETGGWQSWVSI
                     NNIDWPVVLISYYPIYCYHPDCNYNDIEFQTDAVVHEGIHTMFSSMPGRNNKAWFHEG
                     SNCWLQATMELDRSGTTDYSKLSFGWLAAGSIIAPFIPIECYGGWLADGSFGGPNYEG
                     LNDNTRRILGGIQYSEVFPTFLAEYLGYHSIPWIWQNCRGLVLAGMGTVMGEEQMRRI
                     IQEYRARLCLADLGRYQSAVLQMYRDNMGQRIYSEFSSAPAADWKATPYVSTTPGDDG
                     WLIPEERTLPGWTGANIIPLEVEGESVTVNFDAMKNNMSLQLCYRTAAGKTVYCDPVT
                     GGDCTIVTKVHRPANDVVFAVITNLDYTYSVINQRSRHYSYRLKPGKGVVATADIHSD
                     WFNWSN"
     gene            complement(15734..18016)
                     /locus_tag="PKK03_01750"
     CDS             complement(15734..18016)
                     /locus_tag="PKK03_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83145.1"
                     /translation="MKKIYGNLLIVIMMSSALAGCIKYDTNPDVPWVEPKAVDMSGSG
                     SLKVGFGDDALTTNSTYTWTLSETTMQIDVTVDLDQYVNGDDVIPGGAYEIGNFMLPT
                     ATVNEFAGIFVSELDETTFYAIEPDETVVEEMTSYKPGMWVDGEGYSGGWSTSAMFWQ
                     WYIWEGKLDKDGDEIGYDYSSEDYGNTTGLLVIGSNPANVAAQAGKTVTSKAKIVAGD
                     VTYDWIVNVTFAGEISQKLEYEGMLVRGMGETAVETGSSYEMVADENGIELTFEVNLQ
                     EFIDSEDWQIGGFKADLAAINEYFGSDFEKTTITNFYPVEPDGTAGTAWTSYAPGQWV
                     KADGTATDYSGGAMYWQWYLYEATDYDYDLEGNEGYFLLGANPGNAASLSGESVVSLG
                     KFVVGDKTYNLKVTVNYGEGAVPAENFPDVPLTGAGYPLPYSAGVSGDNQFSWYTDED
                     GVYIFADIYTPKVIEDGAWEVMGFVINPEELQTAFGIEAGSWEISTFYPVEPDGTAGT
                     AWSSYAPGQWVAADGTAANWSTGAMFWQYQVGGTYYDGHSTEGLLLIGTNPDNIADLG
                     GESVVSKAKFFGKDWIVTVRYHSTYPTSGSGNVGPHTYTWALTETGVNIVANCSAALT
                     DESWNWFGFPLNETYINDYYGINIDELSGTIESGQTTNGTDGFYPVSSEGTRLEKWTS
                     YSPGMWFGSDGNAWNWDYKSWGNFWQYYAVTTHDHNIPNLMLFGNFGDVTYNAPGTSG
                     TSRAILNGTLQWNVTINIID"
     gene            18330..20762
                     /locus_tag="PKK03_01755"
     CDS             18330..20762
                     /locus_tag="PKK03_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNX83146.1"
                     /translation="MAVLLLLCSCGSENNSQPITEVPFTRVRLSDSFWAPRIEINRTV
                     SIPSAFRQCEVNGRFDNFALAAGLIEGEHKGDFSFDDTDPYKIIEGASYSLAAHYDPL
                     LDHYLDSVIGLIAAAQEPDGYLTTCVTNKCERLSGWWGSSKWERINSHELYNSGHMYE
                     AAIAHYQSTGKRTFLDVALKNADLICEVFGPGEGQKHVPSGHPIVEMALCKLYKVTGE
                     QKYLDLAKYFVEETGRGTDGHALSEYSQDHMPILEQEEIVGHAVRAGYLYSGVADVAS
                     LTGDKDYFTAIKRIWENMAGRKLYITGGIGSRPQGEGFGPDYELHNMENYCETCASIA
                     NVYWNYRMFLATGEAKYADVYERAMYNGVISGVSLSGDRFFYDNPLESMGQHERQPWF
                     GCACCPGNVTRFMASVPGYMYALQGEDIYVNMYAAGRAIIETPGNRTSIVQTTEYPWD
                     GRIAITVSPDKTSRFSIKFRIPGWAGDRPAGTDLYTYTSKATPVTISINGKKIRGRGK
                     DGYINIDRTWSSNDVVELVLPMEVRRVRANDRVKADAGKLAIERGPVVYCLEGCDQPD
                     GEVFNKFIPADAVFEAVYDKDLLNGVVKLEGPAAQVDTEGRVTESTCTAIPYSTWCNR
                     GAGRMAVWIPTGADGAHPRPVPTIASKAYAIKLTGEPNGWPGFNSPALYRSGEADIPG
                     IPVWGVNDQWEPSGSSDIGKPYYCWFGNRGEDENVAYLFEKPETVSEVQVYWLDFDHY
                     DVNYRVPQSWELFYLSDSDQWLPVDKRGDYTVEKDRYNTLKFSPVNTRGLKITARPQK
                     GASGGIIEWKVQ"
     gene            20805..23885
                     /locus_tag="PKK03_01760"
     CDS             20805..23885
                     /locus_tag="PKK03_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX83147.1"
                     /translation="MNVFLQIKKEKTGIRLLSVVLCFLLFICTGGNASAGQGGAMAPA
                     VRITGIVIDEAGEPVISANVIEKGTVNGTATGLDGRFTLEVSGPESVIVVSFLGYLSQ
                     ELTVGSTRDFRVVLKENVSVLDEVVVIGYGTQKRADVTSSVASVKTEAFNKGAILDAG
                     QLVQGKVAGLQISLPSGNPTASTSVLLRGFSTITGSTDPLILVDGVPGSFSTVAPEDI
                     VSIDVLKDGSSTAIYGTRGTNGVIIITTKGLQKDMAPTIEYSGYVSASSWLKRPDFLN
                     ATELREKWAEGKSWSGANEADFGSDVNWLDEISRNAISHVHNLTLRGGSTQTGYLATL
                     TYDQKQGTMKTSGADNIRARLQIAHSMFDNKLSASFSMIANEEKTDMPNNYDYIYRMA
                     CIQNPTQPVYDEDGNYVERKVYFYDNPVSYLNERIGMTRSRNLRATGGLEFKPTDALT
                     FKAMYTRKGQGYLSGYYYTKKDVSNTEGGDNGYASRYTSDFLYDMAELSANWNRTLGG
                     NHHITAIVGYNYENSTWESFGASNKNFPSDSYTYNSLGRGLGIKEGLASVSSSKSNTK
                     LIGLFTRATYNYDDRYLLMASFRREGSSKFGANNKWGNFPGISVGWRINNEDFMSDVT
                     WLDNLKLRAGFGITGIDASSNYESLASLDYSQYFFYNGNYIQTLEPVRNANPNLRWEK
                     KNEYNVGFDFSVFNSRLNGSVDVYQRDTKDGLYNYEVPSPPYQYRYIYANVCEIRNRG
                     IEVMLNAVPVQTADFNWNTSLTYSSNKNELLSLQNDEFQLTTDYFDTGYTGEPIQTST
                     HRVKEGWALGNFYGLRSVGLNAGGYWLVERYNYEDGVPVSKYYDLAENAGSEDWQILG
                     NGVPKYFLNWNNNFRYKGLDLAVSMRGAFAFQILNYQKMFYGNPNIQYNMLNSAFDEI
                     DIVDPRTGQKTGEKTTISDSQRYLSYYVENGDYWKVDNLTIGYTFNMADIKNIQNLRV
                     YASVFNVATFTGYTGLDPEVRMTYGSGGSIDPGTDERDKYPTVRSFTFGVNMTF"
     gene            23899..25596
                     /locus_tag="PKK03_01765"
     CDS             23899..25596
                     /locus_tag="PKK03_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX83148.1"
                     /translation="MKKFKIGLLIMAAAAVFCCTGCFNLDEEVYSEITEESFTASQED
                     IVALMGGAYVPLQFIMDWQGLFDCQEEPGDIIITPVRPNGWDDGGTYQRMHKHTWTPE
                     AWQSQNVYETCFFGINRANSVKDEIEAGKYEMDEELKAATVDELRAVRALWYSILLDT
                     HGNVPIVTTFSAELPVQATRQQVYDFVVTEFTEILTAGRLSKTADVLSYGRLNHWGVM
                     MALMRVYLNAEVYTGTPQWGKALDYANEIINSGVYSLAADYSDNFKVELGPSNPEVIF
                     AVPYDGKYYSRTFCQSAKWYPPIARNHFGWDYQTWGGSCANPQFIKSYAEGDPRLEKT
                     WMIGPQMEKDDPNTVVWTCLNYLPSLTCKNDAGESMTNIDFGYRVNKFEKDLETEFYW
                     SNDFPYFRLAEAYLAKAECLLRMNQDEGDAVIAVNTVRQRSVDPAPAITLADLKGNTK
                     IKYGKLGWGSLTLQQWKDVKDGVKTWADFPDLENSQPLIQSGSDGAAVTLGGLYDEWG
                     WEFACEAQRRTQMIRFGTYSTRNWFNHDAITDGHTKLFPIPMNALNSNSNLTQNTGY"
     gene            25701..29843
                     /locus_tag="PKK03_01770"
     CDS             25701..29843
                     /locus_tag="PKK03_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNX83149.1"
                     /translation="MIFEAITPVKLPEKYDIQPEPGPEFRQPAPSGSLQAKTAFLIIL
                     WIASLILPAPCLAADQSIPLHFRHFTSAEGLESNSIASICVDRDGCIWAGTRDGIYRY
                     CGDRFERLLTSAASDFRISDVNYMIIDENNDIWIAANPGLFIYDIEEQKYTRISLIAD
                     DGIRITSSVNTVAEDRDGNKWFSTYGDGVLKYDITTATLKRYTFEGTSNYVDKVFVDS
                     DNIVWAALREKGRRLERLNKTTGTFEHVDLRYDDPRGEDDILTIFEDSRRRLWLGTWL
                     KGVQEFDKHSGEVSTNFNPTGEPVMHIHSISEYEPGKLFIGTDEGLILFDILSGNYEY
                     YVPDYSDPATISDKFVYPVTRDHEGGFWIGTYYGGINYISPYDGLFYGNNCSRFTGSK
                     SGSIVSKICEGGNGDIWIGTDDQGLIRYRPSDGSHRHFMPVPGQNSISFHNVHALCFD
                     GDDLWIGTYTGGINIYNTLTGRFRVYSSNSYGGLENMSAYSIFKDRGSAMWIGTMSGI
                     EVYDRENERFTVVRRTGVTVIDICQDKKGFIWFATQGRGLQRYNPQRDEWKEYLPRRA
                     GAYFPEEIVNSLFIDQSDVLWAGTARGLFRYNPVNDNFEYVAVRERDDHICCIEGDNH
                     ILWLSTTNGLLRFDPDNGNCIAYSKPDGLQSDHFIASSGYKASDGRIYFGSVNGFNSF
                     FPHRIIIDNTVPDVVLTGLLVNNETVKASDKTILQKAVNFQDKIELKPDENNIVISYS
                     AISYYAPGKISYSYRLKGYDEEWNYVSNNRTATYTNLSPGNYEFQVRSSNGNDLWGDD
                     YKAIQIYIRPPLMFSPLFIILYIIFSILLLIWLFVFLIRRSEKKNRHKMELLNQQREK
                     ETYDEKIRFFTVIAHEIRTPLSLITAPVERILMMPETRVAKIRDDLNTIDRNSRRLLS
                     LVNQLLDFRKIEQDPDIKLDFTRRNIPALLNSIVETYMPYMTQSGMKVMVSGMEDFEA
                     EVDLEAVTKIMSNLLTNAAKYAGSMVELKCIRNEAEQNFQIIVDDDGCGIPHNERDNI
                     FKPFYRLSESKSGTGIGLSIVKSIVSLHNGTVTVDSAPGQGSTFTVTLPLRQPDNKAG
                     EGDGSKPDDILSDESVRACADGRCSLLIVEDNLEMMVFLYDTLAADYNVLTARNGAEA
                     LELMKANSISILVCDWMMPVMDGIELCKAIRGNLYLSHIPFVMLTAKTDVKAKVEGME
                     AGVDIFIEKPFSVRYLCACIKNLLDMRKTMIEKFSRMPLVPLSGVAPNHSNEEFLSHV
                     NNIIEENISNADLSVDLIVRQMYISRSGFFAKIKTLVDVTPNELIQIIRLKKAAALML
                     ENRYLVSEVCYMVGFRNPSYFSKCFQKQFGMTPVEFIKNHVRTSEEAKPVMNVHSGRP
                     GS"
     gene            29935..33549
                     /gene="metH"
                     /locus_tag="PKK03_01775"
     CDS             29935..33549
                     /gene="metH"
                     /locus_tag="PKK03_01775"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNX83150.1"
                     /translation="MKRSVREALAERILVLDGAMGTMIQRHRLTEEQYRGTQYASHPR
                     SLRGNNDLLSLTQPAIIRDIHLSYLGAGADIISTNTFNANRISLSDYGMEPLVYDMNV
                     AAARLAREAVEQHRTESGRESWVAGSVGPTNRTASMSPDVNDPGARTVTFADLAEAYG
                     EQVRGLIDGGVDILLVETIFDGLNAKAALFAISGILEEKGIDLPVMVSGTITDASGRT
                     LTGQTLEAFLITMSHYPLLSIGLNCALGAEQLRPFIEELAAKAPFFVSAHPNAGLPNQ
                     FAEYDQSPEYMGNIIKSFIQNGWVNIIGGCCGTTPDHIKVIASAAAGQKPRSLPERKH
                     VTALAGLEPLVIRSDSNFVNIGERTNVAGSIKFARLIREGNFAAALDVARDQVEGGAQ
                     VIDICMDDAMIDGAAAMRRFINILMSEPDIAKLPIMVDSSKWEVLEAGLQCIQGKPVV
                     NSISLKEGEEDFLKKARLVRRYGAAAVVMLFDEEGQAATFEKRREVADRSYRLLTEKA
                     GFPPEDIFIDPNILAIATGIEEHNNYAVDYIKATEYIKANLPFARVSGGVSNLSFSFR
                     GNDPVREAMHAVFLYHAIKSGMDMGIVNPGLLKVYTSVEPQLLQLTEDVVMNRRKDAT
                     DRLLQYASTMKGAKQEEGTKHPEWRDGDVVSRIKHALVNGIDTFIEDDVEEARPQYDR
                     ALRVIEGPLMDGMNVVGDLFGSGRMFLPQVVKSARVMKKAVAKLTPHIEAEMKGGEAS
                     SAGKVLLATVKGDVHDIGKNIVGVILSCNNYEVIDLGVMVPAEKILDTAVKENVDFIG
                     VSGLITPSLEEMVHIASLMEERGMKIPLLIGGATTSEIHTAVRIAPVYTYNAVYVKDA
                     SRAVPVMSGLVAPDGSLGRSMKEKYEKLRRDHEASRREKRLVTLPAARANGMKTLQPM
                     PAPAMPGIINSGPVSLRTLAEYIDWTFFFFAWKINGKYPDIFNDPVKGAEAKKLYDDA
                     LVMIERIISGDLMVAAALSGIFPAERHGDDVKLDTAHGEAWLRFLRNQEEKEPGQLNL
                     CLADFVAEKDDHAGLFVVSVKTVDQLPADIAGDDYRTIMLRILADRFAEAAAEWLHRE
                     IRMKHWGYSAGEQLTMAEMFKVRYQGIRPAPGYPACPDHTGKKVLFDILRVPETIGVT
                     LTESFVMVPVSSVCGYIFASPGSSYFNVGTIGRDQLEDYANRCGMTVEEAARWLAPNL
                     "
     gene            33559..34521
                     /locus_tag="PKK03_01780"
     CDS             33559..34521
                     /locus_tag="PKK03_01780"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNX83151.1"
                     /translation="MERTTVIDLIRNSKKPLFTFELLPPLKGHSIERIYRTIESLLEY
                     EPAYINFTSHRNEEVLTERPDGLLERRVTRKRPGTIALAAAVKYKYNVNVVPHVLCGG
                     FTKEETENALIEMNFLGINDVLALRGDPPRGSRVFIPNQGGHRNTNELVQQIADMNRG
                     HYLDPDLKNSAATSFCIGVAGYPEKHFEAPNMTSDMANLKRKVEAGASYIVTQMFFDN
                     AKFYAFRDALRAMGVTVPLIPGIKPVSAMTDVNLLPQTFHIDMPEELVHQLSQCRTDD
                     EAREVGIEWAAVQSRDLLKNEVPGIHYYTLGRSDNIARIVKASF"
     gene            complement(34558..35817)
                     /gene="metK"
                     /locus_tag="PKK03_01785"
     CDS             complement(34558..35817)
                     /gene="metK"
                     /locus_tag="PKK03_01785"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNX83152.1"
                     /translation="MSYLFTSESVSEGHPDKVADQISDALLDNFLAWDPESKVACETL
                     VTTGQVVVAGEVKTKTYVDVQSIIREVINRIGYTKAEYMFDGNSCGIFSAIHEQSPDI
                     SMGVERRKKEEQGAGDQGMMFGYAVDETENFLPLSLDIAHELVYQLAEIRREGKQMKY
                     LRPDSKSQVTIEYNDNGTPKRVHTIVVSTQHDDFDKDEKKMLETIKKDVINILVPRVK
                     VQFGKEVQQLFDGKIKYHVNPTGKFVIGGPHGDSGLTGRKIIVDTYGGRGAHGGGAFS
                     GKDPSKVDRSAAYAARHIAKNMVAAGVAREMLVQVSYAIGVAEPVGIFVNTFGTSKVK
                     QSDGEIAKIIDRMFDLRPAMIEKEYGLRKPIYSETAAYGHMGRVPRTVEKTFKSFNGK
                     PDKKVAVQLFRWEELNKVSSIKKAFGL"
     gene            36546..37304
                     /locus_tag="PKK03_01790"
     CDS             36546..37304
                     /locus_tag="PKK03_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX83153.1"
                     /translation="MKFEYSSASEYEVTLSALLKFLDDLGTEIYLAFDEFQSIASYEE
                     GNAEAMLRTMIQPLKNIHFIFSGSNKHMMHEIFHSSKRPFFASTRIMSLSAISDDSYS
                     RFIGDKFAENKRKVSQEAISFVLDWTRRHTYYTQSVCNTLFSTGAKEIDIRLAKTVCG
                     EILDEQEKTFLQYRHLLTSNQWQLLMAVAKEGKVYKPQSGEFLGKHSLGTPAGSKRAV
                     DALLDKEMIYVESDSNGKYYSVYDLFLSRWLERQ"
     gene            complement(37564..38217)
                     /locus_tag="PKK03_01795"
     CDS             complement(37564..38217)
                     /locus_tag="PKK03_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83154.1"
                     /translation="MCKQTVILIALFLSGLPAFSQEKWFDESNGKPGVEIGYLYLYNN
                     LNADNYFKFKYRNATNNGDASIGGISLKLNIPTKLEYLDITVGSIFVKGLDLLEYGYV
                     NPQYADNHDYIINGGGVYFGVSPGIRGKIFSITTEFGIGFFSFKEYVGIVNISRDPFA
                     DEHNLKASYGPGAISSVSLGIRIWKLGINPSVVAIYSGGAGASFTFYGFNLPITFQF"
     gene            complement(38230..>39372)
                     /locus_tag="PKK03_01800"
     CDS             complement(38230..>39372)
                     /locus_tag="PKK03_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNX83155.1"
                     /translation="EPDEPVIVISENERMIIEPDPDNPVKPSAPTNLIGTQTESGIRL
                     TWTMPAGTDPSNTSGYYIYRKTTGSPAYSLTQNVYGAYNRSYDDNNVEASRSYSYYVA
                     AYYQGLTSDPSNYISITAPNYPKPPTSFDAIQQTRNEIELRWENDHTQYIQETRLSKY
                     IVGVTPGYQFYKSFSPNEHNYIDTEVIAGKTVNYKINHVTSFGESNAKYDFVKVPYRD
                     ISQESPVYIKQIKFTDWTLERWPAGKPEFYIAVSNVDAGKEPYTVQEQIDCQFVDKTN
                     VSQVFYGKEVLKWKPGFWYDMLTFTVLEYDKPSSKYTFEISVAYNQKDEKKQGLQSTG
                     ASDFEITFEDHGEKCGNSYLNYYDQPERWLVFPNYGVQIRVSESDN"
     assembly_gap    39373..39472
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<39473..39764)
                     /locus_tag="PKK03_01805"
     CDS             complement(<39473..39764)
                     /locus_tag="PKK03_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83156.1"
                     /translation="MGKETIGINIWLFVAGLVLWTVVQSCEMKEATSNENVKPVGHYE
                     ENEYNSDFSDFARVVSEALKSDKDFRYMVRDEALQLIDGDYDVLLRHFVDKQV"
     gene            complement(40260..40970)
                     /locus_tag="PKK03_01810"
     CDS             complement(40260..40970)
                     /locus_tag="PKK03_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX83157.1"
                     /translation="MSIVVQNVTKNYGPQKALDDVSFTIPAGEIVGFIGPNGAGKSTM
                     MKIITGFFPADAGQVTVCGLPAGPSAREMRRQIGYLPENNPLYLDMYVKEYLGYVADI
                     YSPGRKSREAVSSVIERTGLTPEQGKKIGALSKGYRQRVGLAQALIHDPKVLILDEAT
                     TGLDPNQIVEIRNLISEAGKEKTVMLSTHIMQEVEAICSRVIIIDHGRIVADGPLDEI
                     SSLAGARLEETFRQLTGK"
BASE COUNT         9943 a        10136 c        10992 g        10048 t
ORIGIN      
        1 cccattgaat atttactcct gcgcctcaaa tataacctgt ttgatcctta tacaattgaa
       61 catttatcaa cttacgatat acccttattc acaatgacga tcatctggcc agtactattt
      121 tctcgagata tctcttttgc ccggtcacca cctccactat caggaggcct ccgttacccg
      181 aggcctggaa ttccttaagc tctccggagt acagacgctc attgtctgac atgaatacaa
      241 ccctgccggt gatatcgaat atcctgactt tgccggcggt gccgtcccat ccgcttccct
      301 gcgggagaat gcacaccctg cccggggcgc tgtaaatcct aagagatgtc tcctgtatta
      361 cttctggttt gttttcttcg cctgcggcga tggccttttt ctctgccggg atgatgtcaa
      421 gtgtgaaacg tcccgagatt gtcccctctg cagcctggaa agagtatccg gcttttccgt
      481 caagacctgt cctgctcccc gtcagcctgt cagtcagaac gacagtgtat ccggcgaagg
      541 tctggaactg gctgccgctg atcttgtatg tcccagcctc gtgaatcccg agggtaagtg
      601 gtatggaagt cttctgcgtg ggccatggta tagcgttgat ggagtagttc tccccgcctg
      661 tttcagaata gatctttatg tgacccttta cgtggctgaa tggtttggat gcgtcgagaa
      721 gcccgtcgaa accggtggtt gcttccggat cgaatctcac caccgtttca tcggtctgtg
      781 tggatgatga aaactgcagt cttataagtg ggatttgttc cgccgatttg aacctcggtg
      841 cggcagtgtg ttcgcgggcg ttgtcaggga tggtgatgta tgtccctgtt gcgcgtgttc
      901 gcacgaagaa tccctgcagc ggtgcaatat gtgccgtggc gccgttcgtt cccacaccat
      961 ttacatatga ggcctcttcg ccagaggcgg tcctcaggta atatgcatgc cttatgagtt
     1021 tatgctcatg tgagtaggtg accaggtccc agttgatgcc gcatgtaagt gagttgccga
     1081 ggagggagta gccaaaaacg gttgtgtcac ctcccgagcc gctgaacgga aggtctatcc
     1141 tgcccatgga ggtggtaaga gacttgaggt tgcggtaggt catgatcacg tctgaagcaa
     1201 cgcttacatc atagccctcc ttcgcgttca ggttgctgaa tggggtggtt ccgccgtacc
     1261 cgtcatgcca ttgccagccg tcagacacgt cggtcagcac tttggattcg tcatagcgta
     1321 ccagcaactc gggctctata tcggtaaagg ctgtttttga cactgttgcg ggtgaggcga
     1381 tatagtgcca ccattcgccg gtgggacctg acgtcaggaa caggtcgacg tttgcatttc
     1441 cgccagcacc tgaatagctt ccggtaataa gtgttgcgta ggcattggca tcagcgcgca
     1501 gaacaagggt gccgttgttg gtcagtgagg agagggttac ccttgcaccg ggctcgataa
     1561 tcattgtcag tcctgcgttc agggtgaagg agggaccggg tgcaacagtg ggataagttg
     1621 ccactcctgc cgggatgatt acgtcggtgg ttcccgggcg cactcccttg tcccagccca
     1681 gcggacgggt ccagtcggtt gtccttccca cccacctgct gtaccttatc tcataggctc
     1741 cgatatcggt ttgtccgatc cgctggccgc cggcatagtc ggttaaaggc acggtaactc
     1801 cggggtagct gtcagtcccg ctgtcgcggc agggagaaat aaaggcaatt gtaaaatcgc
     1861 cgcttcccgg atttgtgaag ttcggaccct ccgggtcagt gttggaactg ttcagggtca
     1921 aacatcctgc cagtgaagtc ccttcgatgg cgcagtattc aatgactgat gaaggagtgc
     1981 cggaaaggcc gatattgtct gagcttcccc agatgattgt atttattatt ctcgcatcgg
     2041 gggagttctc gaggtagact gatttgccgc cggctgcagg agaagaacta taagctatgg
     2101 tggtatttat tatcttcggc gagcttgcta tcgaggctac tgatcctccg cttcgtgtgg
     2161 tcgagaggga gctgttgctg tagatgatgg tgtttgtgat taccggggaa gagcctccgg
     2221 cgacgtagat ggcgcctccc tgttcgatag cggagtttcc ggttatgatg caacctgata
     2281 tcacgggcga agtggttccc gcaactatta ttgcaccgcc gtaaccggcg atgcagttcc
     2341 ggatggtggt gctggtgacg gtgacagagg atgttcccag atagagtccg cctccgtagt
     2401 agccgatcct ggtcgctaac cgccggccgt tctcaatggt acagtagctg atgttgctta
     2461 cccctgagga tatcagggtg atatttcccc atgtgtccgt cggttcgccg taagtgccgt
     2521 caaggtcgct gttggaagta atcagtatcc tgcttgaaga ggtcccctgg gcattgagct
     2581 gaccggtgcc gttaacaagt atcccggagg taccggtggc ggcaatgagt atcgttcccg
     2641 gagcgatggt aagctgttcg ttatcaataa taattgtccc ggtgaaatat ttggtgccgg
     2701 agatggtgaa tgcgcctgcc agggtccccc tgtaaggatt acccatactt ccatcaccca
     2761 ggaattgtgc tgttaccggc aagaactgaa tgacggccag gacagaaagt aacagtattc
     2821 ttttcatgtt aaacccctag tcttatgata cattgcttcc cccaaaaaaa tggctttgac
     2881 ctatctatta ttaaactgac agaagactga attgttgcaa aagtaagaaa tctcatccta
     2941 ttggcgatgt tatgattgaa tttataagtt tcggctctgc cggattggaa tgacacttct
     3001 tgctatattt gtgtatatca ccatcggatg caaccggaga ggaacaccat aaggcgtgcc
     3061 cttggcctcc tggcggagag ctctgcaagg gagactgcaa taagcatcat tattctgatc
     3121 atcagggcat tattaccgct gttggctgtg ttgttgctgc ggtatttcgt tgaccaggtg
     3181 acaggggctg ccggttcggg gatggcagag ggaacagcat cagaggcttt cagtgcggca
     3241 ccgggaatgg aaacagtctc cgggattgcc ggttcgggga cggcagaggg aacagcatca
     3301 gaggctttca gtgcggcacc gggaatggaa acagtctccg ggattgccgg tccggggacg
     3361 ggagcgggaa ccactgctga ggctgctgcc ggaaccgggg atctcccatt gggcggaacc
     3421 ggaagtctcc aattgggcgg aaccggaagt ctcccccggg gcggcgacgg aaatctcccc
     3481 tggggcggcg ggatgccggc aatcctcata tggctcatag ctgccatggc cgttgccctc
     3541 ctggctgacg atctgctctc ctatgccggg acctacatta ccaggcggca ctccttcctg
     3601 gttgaggggc atatctcatc gctgatacat aatcatgccg gtatcctggg actgaagtat
     3661 ttcgaggatc cgctgttcca tgacagactg gagcgggctg caagggacat ttcatggagg
     3721 cccgcagcgc tggtgtcaga cttaatcctg ctgctgcgcg gggtgatctc cttcatcgca
     3781 atggcttttg tcctgaggaa cttcgggctg gtcccgctgc tgatcctggt agctgtcttc
     3841 atccccttcc tgctggtcag ggcccgcaac tcttcgaagc tctgggaggc ccgcaagaag
     3901 gcgacggcag attcacggca ggcgtcatac ttcagctggc tgctcaccgg agagaaaccg
     3961 gccagggagg tgaagctctt tgacctcggc ggttacttcg acagtctctt caggaagcac
     4021 ttcagggcat caaaggagcc ggagcttgaa gcggtgaaga gaaacatcct tcccgaaagc
     4081 ctcgcttcgc tgctcaaggt ggctgctttt gccggggtat tgatctatgc aacgtcgcgg
     4141 tacctcgcct cttcaatcac tgccggcgaa ctggcaatgt accttgtggc attccggcag
     4201 gcgctggttt acctacgcga tactgtcacc ggttattccg gacttaacga gaacaggctc
     4261 ttccttaatg atctcttcag cttcctcgat ctccggcccg aatcggaggg cggaagcaag
     4321 gcgccggacc ctggatcatt cagggagatt gcagcggaga aggtgacttt tacttacccg
     4381 gggggtgcgc tgcctgcgct tcaggatgtg actgtcagga ttggccgggg agagaaggtg
     4441 gcaatagtag ggccaaacgg atcgggcaag accacgctgg ttaagctgct ctgccggctg
     4501 tatgagcctg acaagggaca ggtcatggtc aacggtgccg atgcttcagc cattgaaccc
     4561 ggatcatacc ggaagctttt ctcggtggtg tttcagaatt tcatgctcta ttacctgagc
     4621 gcaggtgaca acataaggct tggagacaga tggccgtcag caattggcgg tcagcagcat
     4681 tattcccggg tgaagacggg atccggcaca aataactgca gtgatgaagc cgggggtgcc
     4741 gtgactgcaa ccggcaggaa ggcggaagag gaaatacagg aaccggaaag catggctgaa
     4801 gtgcggagag ctgctgccac ggcaggaata ggcgggctcc tggaagcgct cccggaaggc
     4861 tatgacacca tgctcgggca tcataccgaa ggaggacggg aactcagctg gggcgagtgg
     4921 cagaagatag ctatcgccag ggcagtttac cgcaaatctc ccgtcctgat acttgatgag
     4981 ccttcctcgt cactggatgc cgacgctgaa tacgagatct tctctgacct ctcccggatc
     5041 accggaggca ggacatgcat cttcatcagt cacaggctct ccattgtgcg tgatgccgac
     5101 aggatcattg tgcttgatgg cgggaggata gtggagaccg ggacacatga agagctgatg
     5161 gctgcggaag gaaggtattt taccatgttt accaggcaga aatcaatgta cagatgaccg
     5221 ggttggagaa agaagcgccg gcagaaggca acgaaggact ccagagggca gtgagcttac
     5281 ttctgctgct gagcagaatg gatttctcct ctgagcaaaa gagactgata gcagaggagc
     5341 ttaagtcgtt taatgagtgg aaactgttag ccggattggc tgtcaggcat ggtgtggcag
     5401 cgctgatctg gcagaattta tctgatctgg ggcatgccgg agaggtgccg gcgacggaaa
     5461 ggactcttct ggaggggttg aggtttaaat cgatagcccg cgtatcatgg atcactgaag
     5521 ctgctgccgg ggtgactgcc ctcctggaac aagagggcat aaaagtggtg ctgctcaagg
     5581 gcctctccct tgagcatact gtctacggct cgaaaggact gcgccagatg agcgatgccg
     5641 atctgcttgt cgcaccttct gatgcactga gagcaaggga catacttgtc agggaaggat
     5701 tccggtccat gtccctgaaa tcgccgctct acaggcacat catccttgac cttggcaatc
     5761 acctgccaga attgcatcgc ggaggtatct ccgtcgatct gcactacagg cttttcggcc
     5821 cggagggcga agagatggtg gagagggcga taagggactc gattattact accggggttg
     5881 accgggaacc gggccgtaat gttgctgtga ccagggaatc agaagctgtc catgatacgg
     5941 aaatcggccc cggactggcc cgggaatcag tcgccggtgc cgacaccggc tccttagcca
     6001 gcagcagagc aggtatcccc ggcaccggaa catttcgcat ccttcccccg atgacggcat
     6061 tcctcgccct ggtaagccac atctacaagc atgagataaa aggggagttt cagttgagac
     6121 tctggacaga catatacctg ttgctggacc ggtatggcaa acaaatactg atatcccggc
     6181 tggctgctgc ggcggaagag gcgggcatca gaaaggagac cgctgctgtt ctcacagtaa
     6241 tggagcaggt atggggagtg gcactgcctg agagcatggc cgctgacagc tctgctgccc
     6301 aggaggctgc cgtggccagt ttcatgaaca ggctggccca tccgggatca gtcgaatcca
     6361 tcagtcagcg cgagatgttc atgaggaacc tgaaggcgct gaagagtccg cgaaaaaagt
     6421 ttattttcat tcttggcgat attttcccct ccgtcggatt catgaaaagg aggtataatt
     6481 gccggtccaa agtggcagct tttctgtttt acccacacag gttaggtaaa attccgtgga
     6541 ttttaggtct cctgcggaca gaaaaatacg acaattaata taaaatagaa ataatcgcta
     6601 aataatttca atttttcttg gaaactgacg agatttatta ttaattttat atcagtcaag
     6661 tttttctatc agtcaatgtc atgtcagggc ttatttagat aatgggttaa cgagcaaaaa
     6721 agatgggagc tatgtgtctg cttttttggt tttataggtt ttaactggca agggcgcttc
     6781 agctgcgcag tctcgctgca tgaaccgtgc tttgcgaatg ctgccggcac ttccacggca
     6841 gctctgcccc gggttacatt gacccggggc agttttttct agcctgctga tgccgccggt
     6901 tgccgggggt tgcctgcggt tgccgggcgt tggtgccgga tgccggtggg gagagggagg
     6961 tgccctggtg cttcaattct ttatgccttg cgggcgtttc agcggtattt cattatgccc
     7021 tgcgggcaat tcaccggtat ctcatgatgc ccttcaggca agtggttttg tgacgccatt
     7081 atgctgcaaa agcattactt ttgcacaaac agccgatgag atgaaaagtg ccctttacct
     7141 atacaatacc ctcacacgca aaaaggaact gtttgaaccc cttgccccgc cctttgtggg
     7201 aatgtatgtc tgcggtccca ccgtctacag cgaggctcat ctcggccatg cccgccctgc
     7261 ggtgaccttc gacgtgctgt acaggtacct gatgcacctc ggctacaatg tgaggtatgt
     7321 gcggaacata accgatgtag ggcatcttga gaatgaaact gatgacacag gagaggacaa
     7381 gatcagccgc cgtgcccgcc aggaacacct cgagccgatg gagatagtcc agaaatacat
     7441 gaacagcttt caccatgcaa tggagaagct aaacactctg cctccgtcca tagaaccccg
     7501 cgcaaccggc cacatcatcg aacagcagca gctgactgaa aagcttatcg gcgagggtta
     7561 cgcctatgag gtgaacggtt ccgtttattt cgacgtggaa aagtatgacg gcaggcataa
     7621 ttacggcatc ctttccggac gtgtacttga agatatgcgt gccaacagcc gcgcactgga
     7681 gggacaggac gagaaacggg gccagttcga cttcgccctg tggaaaaagg ctgcaccagg
     7741 acatataatg agatggcctt cgaaatggag cgatggtttt cccggatggc acctcgagtg
     7801 ctcggtgatg agcacgaaat atttaggaga gacatttgat atccacggcg gcggaatgga
     7861 cctgatcttc ccgcaccacg agtgcgagat tgcccagagc accgctgccc agggacacca
     7921 gccggtgcga tactgggtgc ataacaacat ggtgacaatc aacggccaga agatggcacg
     7981 gtcactcggc aacttcatca ccctcgagga gatgttctcc ggatcgcata agcttctggc
     8041 cagggcatat tcacccatga ccatccgctt cttcatgctt caggctcagt accgcagcac
     8101 gctcgacttc agcaatgagg cgctgctggc agcctcgctg ggactgcaga aactgatgaa
     8161 tgcggagaaa actctggccg ggctgatacc tggcgggagc tccactgctg atataagcgg
     8221 catcgccgcg aagtgccatg aggccatgag cgatgatctg aatacaccca tcgcaattgc
     8281 ccacctcttc gacgcagtca ggatcatcaa cctggtcaac gatgggaagg agaagctttc
     8341 ggcagaggat atcaggtcac tgcaggaact cttccgcata ttcatacatg atctcttcgg
     8401 cctcagggcg gaggagtcgg ccggcaacga caggctggaa ggcgtcctga aactgctcct
     8461 tgacatcagg caggaggcga aggcgaagaa ggagtgggct gtctctgacc gcatacgcga
     8521 caggctgagc gaactgggga tcacggtcaa ggaccgcaag gacgggtacg actgggagat
     8581 aacggcatga gcctcacccc ttacaaatca gatattattc agatcggcag cctgacaatg
     8641 gggggcgatc tccccgtacg cgttcagagc atgaccaaca cagacaccct caacacggct
     8701 tcgagcgtca gccagtgcat ccggatcatt gaggcgggag gtgagctggt gaggcttacg
     8761 gcccagggag tccgggaagc ggagaacctt gctgccatca agagcggact tcgggcggca
     8821 ggatttgaaa cgccggtgtg cgccgatatc cacttcaatc cgggtgctgc cgaggtggct
     8881 gcacggatcg ttgaaaaggt acgcatcaac cctggcaact atgctgacaa acgtgcttca
     8941 ttcatcaggc aggagttctc ggactccgag tggaaggcag aactggaacg caccagggag
     9001 agactgatgc cgctcataaa gatatgtact gaatatggta ccgctgtccg cataggggta
     9061 aaccacggct cgctctctga ccggatcatg acccgctatg gcaacacccc cgaggggatg
     9121 gctgtgtcgg caatagagtt cctgaagata ttcaggggag aaggattcaa caggatcgta
     9181 gtctcaatga agtcttcaga cacactcacg atggttatgg ccaaccggct cctggtgaag
     9241 atgatgtttg aagaggggat gcattacccc attcacctgg gcatcactga ggcgggcgag
     9301 ggcgaggacg ggaggataat ctccgccgcc gggacgggta cgctgctggc tgaggggata
     9361 ggtgatacgg tcagggtatc gctttctgag ccgcccgagg ccgagatacc tgttgcccgc
     9421 gcaatcataa aggctgttgc cggtgaggca ggccgggtga agaatcctgt tgcgccgctg
     9481 gagaagcgaa ggccggggga gagatggttc ccgcaggtat atacccgtga aagagaccgt
     9541 ttcaacgatg agtcaggcgc accatacaca ggtgatgtgc tgaccgcctc acctgatgag
     9601 cttcaggcta cggagggcag gcaggcttat gaccggattc tgaatcccgt tttcaactgc
     9661 gacgatccgg aacagctggc catagaagct gccgcgctgc tcggaagatt ctttattgcg
     9721 cgccatccgg cgggactctg catatcaaac agtggtgcag ttcagggaga tgcactgcga
     9781 aggctggcat tctccgttct tcaggctacc gaggcgagga tcacccgcac caggtacatc
     9841 tcatgcccca cctgcggccg gacgaagttt aacctgcagg aggcggtgcg taaagtgaag
     9901 gaggccacag cacacctcac cggaatgaag atagcagtga tgggatgcgt ggtcaacggc
     9961 cccggcgaga tggccggagc tgattacgga tacgttggcg ccggagaggg gaaagtccac
    10021 atctaccggg gaaccgaagc tgttctcaaa aacgtgcctg aagcagaagc acttgacaaa
    10081 cttcttgagc tgatcaataa cgatcaggcc aggcagtgac cggattaaac acttcagtgc
    10141 acccccggta tttgtcttat caggttattc cttctcagtt gaaccagcca gccggtaatc
    10201 aagttgcctc ttctgaaggt cggctttcac cacctcaacg ctgatcttgt cgccaagcat
    10261 gtaaaccttg cctgactgcc ttccgcgtat gcagtagttt tcctcgtcat attcgaagta
    10321 atcatcctgc aactccctga tggcaaccat tccctcgcac tggttctcaa tgatttcgac
    10381 atagatgccc cattcggtca cgccggagat cacgccctcg tacactttcc cgatcttgtc
    10441 gctcatgaac tccacctgct tgtacttgat cgatgccctc tcggcgtcgg ttgcaaggcg
    10501 ctccatctta gaggagtgtt cgcacatttt ttcatacttg tccttatcgc gtgccggttg
    10561 tccttccaga tattttgtca gcagcctgtg caccatcatg tcggggtacc tgcggatggg
    10621 cgaggtgaag tgggtataat gcccgaaagc gaggccataa tggccgatgt tgtcggtgga
    10681 atagaccgcc ttggccatgg agcgcagggc gagggtctcg acgatgttct gctctttctt
    10741 tcccgagacc tcctccatca gcttgttcat tgctgccggg aggcccttga cgtttgcagt
    10801 gtcaatctca tagccaaacc gcttgatgaa gtagctgaac gagctcagtt tctcaggatc
    10861 gggcttgtca tggatacggt acacaaatgt ttttcctttc agttttctgc cgacatattc
    10921 agccacccgc ttgttggcaa gcagcatgaa ctcttcaatt aactggttgg aagttccgaa
    10981 ctcacggaag agaatgccca ggggcactcc cttctcactc aggttgaact tgacctcgag
    11041 cctctcgaag gcgaacgaac cggaggcgaa acgtttgctt cgcagcagct gtgccaggtt
    11101 gtggagggtc agaatctgct ccttcatatc accctcacct gtgtcaataa ccttctgggc
    11161 ttcgccatat gaaaaccgca tgtctgaact tatgacggtg cgtccgaacc actcattcac
    11221 aacgttagcc ttcgcatcca tctcaaaaac agctgaatag gtcagcttgt cctctgacgg
    11281 gttaagcgag cagatatggt ttgagagcct ctcagggagc atcggtatgg tgcggtcgac
    11341 caggtagacg gaggttgctc tttcctgggc gactttttct atcggtgagc cctgtctgac
    11401 ataatgggtg acgtcggcga tatgcacacc gacctcgatg tttccgttgg gcagtttcct
    11461 catcgagagg gcatcgtcga agtccttggc gtcctcaggg tcaatcgtga aggttgggat
    11521 ttcgcggaaa tcacgcctcg cggatatgtc ctcagcagta atctcagcgg gaatcttttc
    11581 tgccgcagcc tcgatctcgg gatcaaatct gttgggcagc ccgaactcgg ccaggatggc
    11641 atgcatctcc gtatcattct cacccgggtt gccgagcacc tctattatct cgcccaccgg
    11701 gtttcgcgag cgctggtccc actccactat acgcgccacg gccttctgtc cctgctttgc
    11761 cccgttgagt tttgcgaggg gaataaagag gtcgaacggc atgttcttgt tgtcgggtat
    11821 aaggaatgcg aattgtttgg tgagctctat cgatccgaca aaggtagctc gtgaacggtt
    11881 gatgatctct accacttcgc cctcggggcg cttccccttg tggcgggcat acagcagcac
    11941 cttcacctgg tcgccgtgga gcgcggtatt aaggtttcgc gctgcgatga aaacatcatc
    12001 ttccatctca tcggtgatca cgaatgcgta tccgaatttg gtaaggtcga ttgtaccggt
    12061 tacatagccg ccgacggcct taagtacata tttgccgtgc ccacgctcat cggcagtgcc
    12121 gtccttcttc atcctgagaa gggcttcaga gagcgcccgc ttttcctctt tttctgttat
    12181 ttttagcttt gcggcgattt gcttgtagtt taacggttgt cttgggtttt ttgccatcgc
    12241 cttgacgatg agctcaataa gttttttctg atcgatcccg cttttttcgg gtggtttttt
    12301 tcttgacatt tctgctttgt ttagcgattc caaagataga ttgaaattgg ctatctttga
    12361 gttgattata tgataatgat ttcactctca tccataccct cgcactcgcc ctcggtggtg
    12421 taccgcagga tcaatgatga gtacctgctc attccgctga cgggcaacat tgccgacatg
    12481 gattctctgt acaggcttac cgagacaggt gcttttatat gggagttgat tgacggagag
    12541 cgagccatca gtgatataac gtcaaagata gtcgaagagt tcgacgttga ggctgacatt
    12601 gccgaaaaag atgccctgga tttcttcagg gagatacagg aattccttca tttcagggag
    12661 acgtgacggt tgaccttgtc atagccgggc ggcggatacg gtttcactcc cgggagggtt
    12721 tggatgttcg tcccgatgag cggttcagtg cgttcacggt tgcagcggag gctgaacctg
    12781 acctgattgt tgatgttgaa cccggttccg gggagatccc tccggcggcg atgcaggtgt
    12841 ttgatgcaca gctgatggag gaggtgcctg gaggggtaat taattcaggc gaatcgttct
    12901 gggagatttt ttcgggagga ggtgtaacgt atgcgcgtgt attcctgaag gagccgcaga
    12961 ggacggctgt cctggaaatg cctcacggcg aaatgcggtg gcggatccgt acgggtgagt
    13021 tcgccatttc acctcagctc tctggcggta aggatcagcc agcagcacaa tttaccgcag
    13081 gaccacccaa taccattaat cctcttccct atccccttga cggacttctg ctttattttc
    13141 tcttctcccg ggccggagat atcatgatcc acggctcagg ggtgtcatgc cacggccggg
    13201 gatggctctt caccggccgc tcgggaagcg gcaagaccac cctggcacag atatttgacc
    13261 gggcaggcga caggatcatt cacgacgaca ggctggtgct gcgccgtgag gagggcaggt
    13321 gggtgatgca caacacacct gtgtaccgca acgatgaacc tcgttctgcc ccactcgacc
    13381 acctgtggat aataaggcat ggatcagcca atgtgtcgga gcccgtcacc ggggcggagg
    13441 ctgtagcaat gattcttgcc aactgtatcc agcagaactg ggacagggag gcggcagcaa
    13501 ggcttgctgc tgcagctgat gacctggcat cgtcagtccg cgtgagcagg ctctcgttcc
    13561 tgcctgaagg aagcattcgc gattacctgc gcctgaggaa ggaggaggac atttcaattg
    13621 ctgcaggtgc tgccgaggct ctgctgaatg caggcaaaat cattactgtc actgcgggag
    13681 gttacagcat gtggcctgcc atcagaccgg gtgataaggt ggagattgag cctattggca
    13741 caagggtgcc ggagattggt gaaatagttg cgttgaagcg cgacggaggg tatgtgatac
    13801 accgggtaac aggagtgaca gccggcagga atgcaaccct catacgcacg cagggtgatg
    13861 gcgttacccg tgaagatgag ccggctgatg ccggtatgat tgccggaatt gtaggatcag
    13921 tggtacggtc cggcaggcag cgacaaccac ccaccagaca cctgccgcgg tggttcaacc
    13981 ggatgacggc caggctacaa tgctggatat ccccgaaagt atgacggcta ccggcaggcc
    14041 cgacggccat acccgcaaat cccaaaaatt cgtaataatt cgcaaatcgc aaatcgcaaa
    14101 tcgcaattct gcagtccctg atgtcctagt tgctccagtt gaaccagtca gaatgaatgt
    14161 cagcagttgc caccactccc ttgcctggct tgaggcggta ggagtaatga cgggaacgct
    14221 gattgatgac cgaataggta taatcaaggt tggtgatgac cgcaaacacc acatcatttg
    14281 ccggtctgtg aactttggta acaatcgtgc agtcgccccc ggtcaccggg tcacaatata
    14341 cagtcttgcc ggcagcggta cggtaacaaa gctgaagtga catgttattc ttcatggcgt
    14401 caaagttcac agtcacgctt tcaccctcaa cttccagggg aattatattt gcgcctgtcc
    14461 acccgggaag ggttctttcc tccggtatca gccatccatc gtcaccgggg gtggttgaaa
    14521 catagggcgt cgccttccag tctgccgccg gggcggaact gaattcggaa tagatgcgct
    14581 ggcccatatt atccctgtac atctgcagta cggcggactg atagcgtcca aggtcagcca
    14641 ggcagagcct ggcacggtac tcctgtatga tccgcctcat ctgttcttca cccatgaccg
    14701 ttcccatccc tgcaagcaca aggccccggc agttctgcca tatccatggt atgctgtgat
    14761 agcccaggta ctctgcaaga aaggtcggaa acacttcgct gtactggatt cctcccagta
    14821 tgcggcgggt attgtcattc agcccctcgt agttgggacc gccgaaggat ccgtcagcaa
    14881 gccatccgcc atagcattca atcggaatga agggagcgat tatgcttcct gcggccagcc
    14941 acccgaatga aagtttgctg taatcggtgg taccggaacg gtcaagctcc atggttgcct
    15001 gaagccagca gttgctgccc tcgtgaaacc atgccttgtt gttccttccc ggcatggatg
    15061 agaacattgt gtgaatgcct tcatgaacca cggcatcagt ctgaaattca atgtcattat
    15121 aattgcagtc gggatggtag caataaatcg ggtaataaga tatcagtacc accggccagt
    15181 caatattgtt tattgagacc cagctttgcc agccaccggt ttcagtattg gaggcatcat
    15241 cggtgctcag tcccgatccg tagagaaaca cggcgctcct gtagccattc tgcactgcca
    15301 tgtcaggagg ccagcccatc acatcacgca tatacgcgaa gtcggtgttc aggcggtcaa
    15361 gcattggctg cactgcctct tccgttacca gaggatttgc ttttgaccct gcgtaaaaag
    15421 accaccagcc gtcagccagt gtccagctga gattacgtcc ggtgtatggc agatttctga
    15481 ccggcatttc ataatccgga taatcatcgc tgaatttata ggaaatcaca gggtcataac
    15541 cgggccattc tgtaaacatg ttttcagcca gcccgttttc atccaccaca atgtttggtt
    15601 caggttcagg tttggtgcag gcactgaata ccagcaggat tgcagcaagg atttgcagga
    15661 agtttctcat aagcaatgaa tagaaagagc ttaccggccg taaccggtaa gctcggaatt
    15721 gattttaatg atttcagtca attatattaa tggtaacatt ccattgcagt gtaccattca
    15781 ggatagccct ggaggtacct gatgtgcctg gagcattata ggtcacgtca ccaaagtttc
    15841 caaagagcat aaggttggga atattgtgat catgtgtggt aactgcgtag tactgccaga
    15901 aatttcccca tgacttatag tcccagttcc aggcattgcc atcggagcca aaccacatgc
    15961 ccggagaata gctggtccat ttttccagtc tcgttccttc tgagctgaca ggatagaagc
    16021 catcggtgcc gttggtagtc tggcctgatt caattgttcc tgagagctca tctatattga
    16081 taccgtagta atcgtttata taggtctcat tcagcgggaa tccgaaccag ttccatgatt
    16141 catcggtaag agcggcggag cagttggcaa caatgttaac tcctgtttcg gtcagagccc
    16201 atgtataggt atgtggtccc acattgcctg agccgcttgt cgggtaggtg ctgtgatacc
    16261 gtactgtcac gatccagtcc ttgccgaaga atttggcttt tgaaacaact gattcaccgc
    16321 ccaggtccgc aatgttatcc ggattggtac cgatcagcag caatccctcg gtgctgtgcc
    16381 cgtcatagta ggttccgcca acctgatact gccagaacat tgctccggtg ctccagttgg
    16441 ctgccgtgcc gtctgctgca acccactgac cgggagcgta tgatgaccag gctgtcccgg
    16501 cggtgccatc cggttcaaca ggatagaaag tagagatttc ccagctgcct gcttcaatgc
    16561 caaaggctgt ctgcagctcc tccgggttga taacgaagcc catgacctcc catgcgccgt
    16621 cttctataac tttcggggta tatatatcgg cgaagatata gacaccatct tcatcagtgt
    16681 accagctgaa ctggttgtct cctgacacac ctgcgctgta tggcagagga tagccggcac
    16741 ctgtgagagg cacatcgggg aagttttccg cgggcactgc accctcaccg taattcaccg
    16801 taaccttaag gttgtatgtt ttatcaccaa caacaaactt acccagggaa acaactgatt
    16861 cgcctgaaag ggatgctgcg ttgcccggat tggctcccag gaggaagtac ccttcattgc
    16921 cctcaagatc atagtcatag tcagttgctt catacaggta ccactgccag tacattgcac
    16981 ctcctgagta atctgtggct gttccgtctg ccttcaccca ctgacccgga gcatatgatg
    17041 tccaggctgt tccggcggtt ccgtccggct caacgggata gaagtttgtg atagttgttt
    17101 tctcgaagtc ggatccaaag tattcattta ttgcagccag gtcggcttta aatcctccta
    17161 tctgccagtc ttcactgtcg ataaattcct ggaggtttac ctcgaaggtc agttctatgc
    17221 cgttctcatc ggcaaccatc tcataggagc ttcctgtctc cacggctgtt tcacccattc
    17281 cgcgaaccag cattccttca tactccagct tctgtgatat ttcaccggca aaagtgacat
    17341 tgacaatcca gtcataggta acatctccgg ctacgatctt tgccttggaa gtaactgttt
    17401 tcccggcctg ggctgccaca tttgccgggt tgcttccgat aaccaacagg cctgtggtgt
    17461 ttccataatc ttcggaactg tagtcatagc cgatctcgtc gccatccttg tcgagtttgc
    17521 cttcccatat ataccattgc cagaacatgg cgctggttga ccagcctccg gaataacctt
    17581 caccgtcaac ccacatgccg ggcttgtagg aagtcatctc ctcaaccacg gtttcgtcag
    17641 gttcaattgc atagaaggtt gtctcatcaa gttccgagac aaatatcccg gcgaactcat
    17701 tcaccgtagc ggtcggcaac atgaagtttc caatttcata ggcgcccccc ggtatgacat
    17761 cgtcaccatt aacatactga tcaaggtcaa cagtgacatc aatctgcatt gtagtctcgc
    17821 tgagggtcca ggtgtatgtg ctgtttgtgg tcagtgcatc atcaccgaat cccaccttca
    17881 gtgaaccgct tcctgacatg tccactgcct ttggttcaac ccatggtaca tcagggttcg
    17941 tatcatactt gatacagccg gccagggcag atgacatcat aattacaatc agaagatttc
    18001 cgtaaatctt tttcatcatt caatatttag gattaaactt ttatttgccg gttgatttat
    18061 tgttataaaa gtaggcccta ttttaaaaac ggacaggtaa caattgtccg aaaaactata
    18121 ccaataatcc aaatgtgaat ccggactaag atgccctaag tacgtgccaa tgggcacgaa
    18181 agcttttctt tattattgga cgatattgtc agcattaagg acaatgttgc tagtgaggct
    18241 tactcttgtt ccataccttt gagttgctac aattcattcc gttatatact atgcatttca
    18301 aactggtcaa tctatttact tatcctttga tggctgtgct gctgttgctg tgttcatgcg
    18361 gctcagagaa caacagtcag ccgataacgg aagtaccgtt taccagggta cgtctgtcag
    18421 attcattctg ggctcccagg atagagatca atcgcacggt gagcattccc tcggcatttc
    18481 gtcagtgtga agtgaacggc aggtttgaca atttcgccct ggctgcaggg cttatcgagg
    18541 gagagcataa aggcgatttt tcctttgatg acactgatcc gtacaaaata attgaaggag
    18601 cctcatattc gctggctgca cattatgatc cccttcttga ccattacctt gacagcgtca
    18661 tcggcctcat agccgcagcg caggagcctg acggctatct caccacgtgt gtcaccaata
    18721 aatgtgaacg gctgtcgggt tggtggggtt cttcaaaatg ggaaagaatc aacagtcatg
    18781 agctgtataa ttcaggtcac atgtatgagg cagcaattgc gcattaccag tctacaggta
    18841 aacgaacctt ccttgatgtg gcactgaaga atgctgacct tatctgcgag gtgttcggcc
    18901 cgggtgaggg gcagaagcat gtcccctccg gccaccccat agtggagatg gccctttgta
    18961 agttgtacaa ggtcaccggt gaacagaaat accttgacct ggcaaagtat ttcgttgaag
    19021 agaccggccg cgggactgac ggacatgccc ttagcgaata cagccaggat catatgccca
    19081 ttcttgagca ggaggagatt gtggggcatg ccgttcgtgc cggttacctc tattccggag
    19141 tagctgatgt ggcttcactg acaggtgaca aagactactt cacggccatc aaacggatat
    19201 gggagaacat ggcaggcaga aagctctata ttaccggagg cataggttca cgcccgcagg
    19261 gcgaaggatt cggtccggac tatgagctcc ataacatgga aaattactgc gagacctgcg
    19321 cctcaattgc caatgtatac tggaattaca ggatgtttct tgccaccgga gaggcaaagt
    19381 atgccgatgt gtatgagagg gccatgtaca acggtgtcat ttccggggtt tcgctcagcg
    19441 gagaccgttt cttttatgat aacccgctgg agtcaatggg gcagcatgag cggcagccct
    19501 ggttcgggtg tgcatgttgc cccggtaacg taacccgctt catggcatca gtgccgggtt
    19561 atatgtatgc cctgcagggc gaagacatat atgtaaatat gtatgccgcc ggccgtgcaa
    19621 taatagagac acccggaaac aggacttcca ttgtgcagac aactgaatac ccttgggatg
    19681 gaaggattgc catcacagtc agccctgaca aaacctcaag attcagcatt aaattccgca
    19741 ttcccgggtg ggccggagac agacctgcgg gaacggatct ttatacctat acttccaagg
    19801 cgacaccagt aacaataagt atcaatggaa aaaaaatcag gggaaggggg aaggacggat
    19861 acattaatat agacagaacc tggtcttcca atgatgttgt tgaactggtg ctgccaatgg
    19921 aggtacgccg ggtcagagcc aatgacaggg taaaggcaga tgccggcaag ctggcaattg
    19981 aaagggggcc tgttgtctat tgcctggaag gctgcgatca gcctgacggc gaggtattca
    20041 acaagttcat tccggcagac gctgtttttg aagcagttta tgacaaagat ttattgaatg
    20101 gggttgttaa gttggaaggg ccggcagcgc aggttgatac agaaggtaga gttaccgaaa
    20161 gcacctgcac tgccattcct tattccacct ggtgcaacag gggtgccggc cggatggcag
    20221 tgtggattcc caccggcgct gacggggcac atccccggcc tgtccctacc attgcctcaa
    20281 aggcatatgc cattaagctt accggagagc cgaacggatg gcccggcttt aacagtccgg
    20341 ccctgtaccg gagcggagag gctgatattc ccgggatacc cgtctggggt gtgaatgacc
    20401 agtgggagcc ctccggctca tctgatatcg gcaaaccata ttattgctgg tttggcaaca
    20461 ggggcgagga tgaaaacgtg gcttaccttt ttgagaagcc ggagacagtt tcagaagtgc
    20521 aggtatactg gcttgatttt gatcattatg acgtaaacta ccgtgttccg caatcctggg
    20581 aactctttta cctgtctgac agcgaccagt ggcttccggt agacaaaagg ggagattata
    20641 cagttgagaa ggacaggtat aacacgttga agttcagtcc cgtgaatacc cggggtctga
    20701 aaataactgc ccggccgcag aaaggggctt caggaggtat cattgaatgg aaagtgcaat
    20761 aatcattgaa accttaataa tataacctat aaatgtttag aaatatgaat gtattccttc
    20821 aaatcaaaaa agagaaaacc ggcataagac tgctcagtgt ggttctctgt tttctcctgt
    20881 tcatctgtac cgggggtaat gccagtgcag ggcaaggcgg agcaatggcg ccggctgtca
    20941 gaattaccgg tattgtcatt gacgaagccg gggaaccggt aatcagtgcg aatgtcattg
    21001 aaaaaggaac ggtgaacggg acagccacag gtcttgacgg ccgctttacc cttgaggtca
    21061 gcggacctga atcagtgata gttgtctctt tcctcggtta tctgtcacag gagttaaccg
    21121 ttggcagcac aagggatttc cgtgtggttc ttaaggagaa tgtgtctgtc ctggatgagg
    21181 tggtcgtcat aggttacggt acccagaaaa gggctgatgt gactagttcc gtggccagtg
    21241 tgaagacaga agccttcaac aaaggagcaa tcctcgatgc cgggcagctc gttcagggca
    21301 aggtggccgg tctccagata agccttccgt caggtaaccc taccgcatca acatcggtgc
    21361 ttcttcgtgg tttctcaaca atcaccggat caaccgatcc gctgatcctg gtcgatggcg
    21421 ttcccggttc attcagtaca gttgctcctg aagatattgt ctcaatcgac gttctgaagg
    21481 acgggtcttc aacggctatt tacggtaccc gcggcaccaa tggtgtgatc atcataacta
    21541 ccaagggact acagaaggat atggctccca ctattgaata cagcggctat gtctctgcat
    21601 catcatggct taagaggcct gatttcctga atgccactga actgcgcgaa aaatgggctg
    21661 agggcaaatc atggtcagga gcgaacgagg ccgatttcgg ctctgacgtt aactggcttg
    21721 acgagatcag ccgaaacgca atctcgcatg tgcataacct tacactcaga ggaggttcaa
    21781 cccagacagg atacctggcc acactgacat atgatcagaa gcagggcaca atgaaaacct
    21841 caggcgctga caacatccgt gcccgcctgc agattgccca ttcgatgttt gataacaaac
    21901 tcagcgccag tttttccatg attgccaatg aggagaagac agacatgccc aacaattacg
    21961 attacatcta ccgcatggcc tgtattcaga atcctaccca gcctgtatat gatgaagacg
    22021 gcaattatgt tgaaaggaaa gtctatttct atgacaaccc tgtttcatat ctgaatgagc
    22081 gcatagggat gacccgttca cgcaacctgc gtgccaccgg tggacttgag ttcaagccta
    22141 cagatgctct tacattcaag gctatgtata cgcgcaaggg tcagggctat ctttcgggct
    22201 actactacac aaagaaggat gtgagcaaca ccgagggcgg tgacaatggc tatgcctcac
    22261 ggtatacctc agatttcctc tatgatatgg cagagctcag cgccaactgg aacaggaccc
    22321 tgggcggaaa ccatcatatc acggcaattg taggttacaa ctatgagaac agcacatggg
    22381 aaagtttcgg agcttcaaac aagaatttcc cgagtgacag ttatacatac aactctcttg
    22441 gccgtggcct tggaataaag gaaggtcttg cctcggtttc atcgtccaag tcgaatacaa
    22501 aactcatcgg gttatttacc cgtgctacct ataactatga tgaccgttac ctgttaatgg
    22561 catcattccg cagggagggt tcgtcaaagt tcggcgccaa taacaaatgg ggtaatttcc
    22621 ccggcatttc agtgggctgg cgtataaata acgaggactt catgagcgat gtgacctggc
    22681 ttgacaacct caagctgcgt gcaggcttcg gtataaccgg tattgatgca tcctcaaact
    22741 atgaatcact ggcatcactt gactacagtc agtatttttt ctataacggc aattacatcc
    22801 agacgctgga acctgttcgc aacgcgaacc ccaacctgcg ctgggaaaag aagaatgagt
    22861 acaacgtagg atttgacttc agcgtgttta acagcaggct gaacggctcg gtggatgtgt
    22921 atcagcgaga caccaaggac ggtctttaca attatgaagt gccttctccg ccctaccagt
    22981 acaggtatat ttatgccaac gtgtgtgaga tacgtaaccg tggtattgag gttatgctga
    23041 atgcagtacc ggttcagact gctgacttca actggaatac cagcctgaca tactcatcga
    23101 acaagaacga acttctttca cttcagaatg atgagttcca gcttaccact gactacttcg
    23161 atacaggtta cacaggtgag ccaatccaga cctcaaccca ccgagtaaag gaaggctggg
    23221 ctcttggcaa cttctacggc ctgaggtcag tcggcctcaa tgccggaggt tactggctgg
    23281 ttgagcgcta taactatgaa gatggcgttc cggtaagcaa gtattatgac ctggctgaga
    23341 atgccggctc agaagactgg cagattctcg ggaacggtgt tccgaagtac ttcctcaact
    23401 ggaacaacaa cttccggtac aagggtcttg acctggccgt cagcatgaga ggtgcatttg
    23461 cattccagat actgaactat cagaagatgt tctatggcaa ccccaatatc cagtataata
    23521 tgctgaacag cgcttttgac gagatcgata tcgttgatcc tcgtaccggg cagaagacag
    23581 gtgaaaaaac cacaataagt gactcacaac gttacctgag ctattatgtc gagaacggcg
    23641 actactggaa ggttgacaac ctcaccattg gatatacctt taatatggcg gatatcaaga
    23701 acatacagaa cctcagggta tatgcctctg ttttcaatgt tgcaaccttt accggttaca
    23761 ccggtcttga tcccgaggtc aggatgactt acggatcggg cggatcaatt gaccccggga
    23821 cggatgaacg tgataaatat cctaccgtac gctcatttac gttcggtgtg aacatgactt
    23881 tctaaaactg accgcaatat gaaaaaattt aagataggat tattgattat ggcagcagca
    23941 gcagtgttct gctgcacagg ctgcttcaat ctggatgaag aggtttactc cgagattacc
    24001 gaggagtcat tcaccgcatc acaggaagat attgttgccc tgatgggagg tgcttacgta
    24061 ccccttcagt tcatcatgga ctggcagggt ctgtttgatt gccaggagga acccggagat
    24121 atcatcatca ccccggttcg cccgaacgga tgggatgacg gtggtactta ccagcgcatg
    24181 cacaagcaca cctggactcc cgaggcgtgg cagtcgcaga atgtatatga aacatgcttc
    24241 ttcggaatca acagggccaa cagcgtcaag gacgagatag aggcaggcaa gtatgagatg
    24301 gatgaggagc tgaaggctgc aactgtagat gaactgcggg cagtgcgcgc actctggtat
    24361 tccatactgc ttgacactca tggcaatgtg cctattgtga ccaccttctc tgctgaactg
    24421 cctgttcagg ctacccgtca gcaggtttat gactttgtgg tgaccgaatt cactgaaata
    24481 cttacagcag ggcgattgag caagactgcc gatgtgctgt catacggacg tcttaatcac
    24541 tggggtgtga tgatggcact gatgcgcgtt tacctgaacg cagaggtcta tacaggcacc
    24601 cctcaatggg ggaaggcact ggattatgcc aatgagatta tcaattccgg tgtttacagc
    24661 ctggctgctg actactctga taacttcaag gtagagctcg gaccctccaa tcccgaggtg
    24721 atctttgcag ttccatatga cggaaaatat tattccagga ctttctgcca gagcgcaaaa
    24781 tggtatcctc ccatcgcaag gaaccatttc ggatgggact atcagacatg gggcggaagc
    24841 tgcgccaacc cccagtttat caagagttat gctgaaggtg atccccgcct ggaaaagacc
    24901 tggatgatag gtccgcagat ggagaaggat gaccccaata cagtggtctg gacctgtctc
    24961 aattatctcc cttcactcac ctgcaagaat gatgccggcg aatcaatgac aaacattgat
    25021 ttcggctaca gggtcaataa attcgagaag gatctggaga ctgagttcta ctggagcaat
    25081 gactttccgt atttccgcct ggctgaagct tacctggcca aagccgagtg cctgcttcgc
    25141 atgaatcagg atgaaggcga tgcggttatt gcggtcaata ccgtgcgtca gcgttctgtt
    25201 gatccggcgc cggccatcac cctggctgac ctcaagggga acacaaagat caaatacgga
    25261 aaactgggct ggggttcact gaccctgcag caatggaaag atgtcaagga tggtgttaag
    25321 acctgggccg actttccaga tctggagaac tcgcagcctc tcattcagag cggaagtgac
    25381 ggtgcggcag tcaccctggg agggttgtat gacgagtggg gctgggagtt tgcctgcgaa
    25441 gctcagcgtc gcacacagat gatccgcttc ggaacctatt caacaaggaa ctggttcaac
    25501 catgatgcca taactgacgg acataccaaa ctgttcccga ttccgatgaa tgcactgaat
    25561 tccaactcaa acctgacaca gaacacggga tattaggatc ggaccaatga attttataca
    25621 aaatggtact tacataacgg gctgcgttag cagtccgtta tagtttgtac cttaccccgg
    25681 aaatcgtaaa ttgcaggctt ttgatatttg aagcaattac ccccgtgaag ctcccggaaa
    25741 aatacgatat tcaaccagaa cccggacctg aattccggca gcccgcgcct tcaggcagtc
    25801 tgcaggcgaa gacagccttc ctgatcattc tgtggattgc atctctgatt ctgccggcgc
    25861 cctgcctggc tgcggatcag tcaatacccc tgcatttcag gcatttcact tccgcggaag
    25921 gacttgaatc caactcaata gcaagcatat gcgtggacag ggacggatgt atctgggcgg
    25981 gtaccaggga cgggatttac cggtattgcg gcgaccgttt tgagcggctc ctcacatcgg
    26041 ccgcaagtga cttcagaatc tctgatgtga actatatgat catagatgaa aacaatgaca
    26101 tatggattgc ggccaatccg ggattgttca tatatgacat tgaagaacaa aagtatacca
    26161 gaataagcct gatcgctgat gatggtatcc gtattacctc ctcagtcaac accgtggctg
    26221 aggacaggga tggcaacaaa tggttctcaa catatggaga cggagtcctg aaatatgaca
    26281 ttacaaccgc tacactgaag aggtacacct tcgagggtac ctccaattat gttgacaagg
    26341 ttttcgttga ctctgacaat atcgtgtggg cagccctgag ggagaagggg agacggctgg
    26401 aacggcttaa caagacaacc ggaacattcg agcacgtcga cctcaggtat gatgatcccc
    26461 ggggagaaga tgacatactc accatctttg aagacagcag aaggagattg tggctgggca
    26521 cttggcttaa gggtgtccag gaattcgaca agcattcagg agaagtcagc accaacttca
    26581 atcctaccgg cgagcctgtc atgcacatcc actcaatatc cgaatacgag cccggcaaac
    26641 tgttcatcgg cactgacgag gggcttattc tgtttgacat cctgtcaggc aattatgaat
    26701 actacgttcc tgattacagt gaccctgcaa ccataagtga taagttcgtc tatcctgtca
    26761 cccgtgatca cgagggtgga ttctggatag ggacctatta cggagggatc aattatatct
    26821 ctccatatga cggcctcttc tatggcaaca actgctcacg gttcacagga tcaaaatccg
    26881 gaagcatagt gagcaaaatc tgcgaaggag gtaatggtga tatctggatc ggaacggatg
    26941 accaggggct gatcaggtac agaccgtctg atggcagcca caggcatttc atgcccgttc
    27001 cgggtcagaa cagcatctca ttccacaatg ttcatgctct ctgttttgat ggtgatgacc
    27061 tgtggattgg tacttatacc ggcgggataa atatctataa tacccttacg ggcaggttca
    27121 gggtatacag cagcaacagc tacggtggcc ttgaaaacat gagtgcatac tcgattttca
    27181 aagaccgcgg ttctgccatg tggataggca ccatgagcgg cattgaagta tatgaccgtg
    27241 aaaatgaaag gtttaccgtg gtcaggagga caggcgttac cgtgatagat atctgtcagg
    27301 ataagaaagg attcatatgg tttgccaccc agggcagggg cttgcagcga tataatcccc
    27361 aaagggatga gtggaaggag tatctcccgc gtcgcgcagg tgcatatttc cctgaagaaa
    27421 ttgtcaactc actgtttatc gaccaaagtg acgtgctctg ggccggcact gccaggggac
    27481 tgtttcgcta caacccggtg aatgataact ttgaatatgt tgctgtacgt gaaagggatg
    27541 accatatttg ctgcattgaa ggcgataatc atatactgtg gctgtcaacc accaacggtc
    27601 tgctacgctt tgatcccgat aacgggaact gcattgctta cagcaagcct gacggcctgc
    27661 agagcgacca cttcatagct tcatccggct ataaagcaag tgacggcagg atttatttcg
    27721 gatcagtgaa cggattcaac tctttctttc cgcaccggat catcatcgat aatactgttc
    27781 cggatgtggt gcttaccggg ttacttgtca acaatgaaac agtaaaggca tctgataaaa
    27841 ccattctgca gaaggcggtt aatttccagg ataaaattga actgaaaccc gatgagaaca
    27901 atatagtcat ctcctattca gccatatctt attatgctcc agggaagatc agttattcat
    27961 accggcttaa aggctatgat gaagaatgga attatgtctc aaacaacaga acagccacct
    28021 acacgaacct ctccccgggc aactacgagt ttcaggtccg ctcctcgaac ggcaatgact
    28081 tgtggggtga cgactataag gcaatacaga tttatattcg ccctcctctg atgttttcac
    28141 ccttattcat aatcctttat atcatcttct cgatcctgct cctgatatgg ctgtttgtct
    28201 ttcttatccg ccggtcggaa aagaaaaacc ggcataagat ggagttgctg aaccagcaga
    28261 gggagaaaga gacttatgat gagaagatcc ggttctttac tgtcattgcc cacgaaatac
    28321 gcaccccgct ctcactgatc accgccccgg ttgaaaggat tctcatgatg cccgaaacca
    28381 gggttgcgaa gatacgtgat gatcttaata ccattgaccg taacagcagg cgactcctct
    28441 ccctggtgaa tcagctcctt gatttcagga agatagagca ggaccctgat ataaaacttg
    28501 actttacacg gcgtaacatt cccgctcttc ttaattccat tgtggagacc tacatgccat
    28561 atatgaccca gtcagggatg aaagtcatgg tgtccgggat ggaagatttt gaagccgagg
    28621 ttgatcttga agcagtcacc aagataatga gcaacctgct gaccaacgca gccaagtatg
    28681 ccggcagcat ggttgagctg aaatgcatcc gcaatgaggc agagcagaac ttccaaatca
    28741 ttgtggatga tgacggctgc ggcattccgc ataatgagag ggataatatt ttcaaaccct
    28801 tttaccgctt atctgaaagc aagtcaggca ccggtatcgg actgagcatt gtcaaaagca
    28861 ttgtgagcct gcataacgga acagtgacag tagactccgc tccggggcag ggtagtactt
    28921 ttactgttac cctgccgctc aggcaacccg ataataaagc cggagaaggc gacggaagca
    28981 aaccggatga tatcctgagt gatgaatcgg tccgtgcctg tgcagacggg cgctgctcgt
    29041 tgctgattgt cgaggacaat cttgagatga tggtctttct gtatgataca ctggcagctg
    29101 attataacgt gctgacagca cgaaacggtg ctgaggcgct cgaactgatg aaggccaact
    29161 ccatctccat tcttgtgtgt gactggatga tgcccgttat ggacgggatt gagctctgca
    29221 aggcaatacg cggcaacctg tacctgagtc acatcccgtt tgtgatgctg accgcaaaga
    29281 ctgatgtcaa agcaaaggta gagggaatgg aggccggggt tgatattttc atcgagaaac
    29341 ctttctcggt caggtatctc tgtgcgtgta tcaaaaacct tctggatatg cgcaagacca
    29401 tgattgagaa gttctccagg atgccactgg tacccctgag cggcgttgcc cccaaccact
    29461 caaacgagga gttcctctca catgtcaata acattattga agaaaacatt tcaaatgccg
    29521 acctgtctgt tgatctgatt gtcaggcaga tgtacataag ccgttcggga tttttcgcca
    29581 agatcaagac cctggtcgat gtgactccca acgaactgat ccagataatc agactgaaga
    29641 aggctgcagc ccttatgctt gagaaccgtt atcttgtaag cgaggtctgt tatatggtgg
    29701 gatttcgcaa tccatcatat ttctccaagt gttttcaaaa acaatttgga atgactcctg
    29761 ttgaattcat aaagaatcat gtcaggactt cggaggaagc aaagcctgtg atgaatgttc
    29821 attccggcag acctggttct tagtgagaga ttatggtgcc aggtcgctgt acacaacctg
    29881 gttttcatat atcacagctt taagttagct ttgctgaact aaaaccgcca gacattgaaa
    29941 agatctgtca gagaggcact ggctgagagg atacttgtcc tcgacggcgc catgggtacc
    30001 atgatacagc gccacaggct tacagaggag cagtaccgcg gcacgcagta tgccagccac
    30061 ccccggtcac tcaggggcaa caacgacctg ctgtcgctga cacagcccgc cataatcagg
    30121 gatattcacc tgagctacct gggggctggc gctgacatta tcagcaccaa caccttcaat
    30181 gctaacagga tatcgctgtc tgactacggt atggagccac tcgtctatga catgaatgtg
    30241 gctgccgcac ggctggccag ggaagctgtg gagcaacacc gcacagaaag cggtcgcgag
    30301 tcatgggtgg ccggatctgt cggtcccacc aaccgtactg cctcaatgtc acccgatgtg
    30361 aatgatccgg gcgcccggac agtaacattt gccgatcttg cggaggccta cggcgaacag
    30421 gtgcggggac tgattgacgg gggcgtggat atcctgcttg ttgagaccat ctttgacgga
    30481 ctcaatgcca aggcagccct atttgcaata agcgggatcc ttgaagagaa gggtatagac
    30541 cttcccgtca tggtctccgg cacaatcacc gatgccagcg gccgcacact tacggggcaa
    30601 accctcgagg cgttccttat tacaatgtcg cattacccgc tgctgagcat cggtctgaac
    30661 tgtgcccttg gggcggaaca actcaggcca ttcattgagg aactggccgc aaaagccccg
    30721 ttcttcgtct cggcacatcc caacgccggt ctgccgaacc agtttgcgga gtatgaccag
    30781 tcgcccgaat acatgggcaa catcataaaa agcttcatcc agaacggctg ggtgaacatc
    30841 atcggcggct gctgcggcac caccccggac catattaagg ttatagcttc cgccgccgcg
    30901 gggcagaaac ccaggtcact tccggaaagg aaacatgtca cagcacttgc aggcctcgag
    30961 ccgctggtga tccgttctga cagcaacttc gtcaacatag gtgagcggac caacgtggcc
    31021 ggctcaataa agttcgcgcg gctgatccgc gaggggaatt tcgcggcagc gctcgatgtg
    31081 gcgcgtgacc aggtggaggg tggcgcacag gtgattgaca tatgcatgga tgatgccatg
    31141 attgacggcg ccgcagccat gagacggttc atcaacatac tgatgagcga gcctgacatc
    31201 gcgaagcttc ccatcatggt cgattcgtcg aagtgggagg tgctggaggc agggctgcag
    31261 tgcatacagg gcaaaccggt tgtcaactcc atcagcctga aggagggtga ggaggatttc
    31321 ctgaagaaag cccggctggt gaggcgctac ggcgctgccg cggtggtgat gctctttgac
    31381 gaggagggac aggctgccac cttcgaaaaa cgcagggagg tggctgacag atcgtaccgt
    31441 ctgctgacgg agaaagccgg tttcccgccg gaggacatct tcatcgatcc caatatcctg
    31501 gccatcgcca caggcatcga ggagcacaat aactatgcag ttgattacat caaagccact
    31561 gaatatatta aggcaaacct cccctttgcc agggtgagcg gaggagtgag caacctgtcg
    31621 ttctcgttcc gtggaaatga cccggtcagg gaggcaatgc acgcggtgtt cctctatcac
    31681 gccattaaat caggcatgga tatggggatc gttaatcccg gactgctgaa ggtctatacc
    31741 tccgtggagc cgcagctgct gcagctgacg gaggatgtgg tgatgaaccg caggaaggat
    31801 gccaccgaca ggctgctgca gtatgcctcg acgatgaagg gagcgaagca ggaggaggga
    31861 acaaagcacc cggaatggcg cgacggagat gtggtgagcc ggataaagca tgccctggtg
    31921 aacggcatag acacattcat cgaggatgat gtggaggagg cgcgcccgca gtatgacagg
    31981 gcactgcggg tcatcgaggg tccgctgatg gacggtatga acgtggtggg agacctgttc
    32041 ggatcaggaa gaatgttcct gccgcaggtg gtcaagagcg ccagggtgat gaagaaggca
    32101 gtggcaaagc tgactccgca catcgaggcg gagatgaagg gcggagaagc ctcgtctgca
    32161 ggcaaagtgc tgctcgcaac agtcaagggt gatgtgcatg acatcgggaa aaacattgtg
    32221 ggtgtcatac tttcgtgcaa caactacgag gtgatagacc taggtgtgat ggtgcccgcg
    32281 gaaaagatac ttgatacagc cgtaaaggag aatgttgatt tcatcggggt atccgggctg
    32341 atcacccctt cgcttgagga gatggtgcat atcgcatcgc tcatggagga gagggggatg
    32401 aagatcccgt tgctgatagg cggggcaacc acctcggaga tacacacagc ggtcaggata
    32461 gctccggttt acacgtataa cgcagtatat gtgaaggatg catcgcgggc ggtgcctgtt
    32521 atgtcggggc tggtggctcc tgacggctct ctcggcagga gcatgaagga gaagtacgaa
    32581 aagctgcgcc gtgaccatga ggcatcacgc agggagaaga ggctcgtaac attaccggca
    32641 gccagggcca acggtatgaa gaccctgcaa ccgatgcctg cgccggcgat gccgggaata
    32701 ataaactccg gcccagtgag cctgcggacg cttgctgaat acatagactg gacattcttc
    32761 ttcttcgcgt ggaagatcaa cggcaaatat cctgatatct tcaatgatcc cgtaaagggg
    32821 gcagaagcga agaagcttta tgacgatgcc ctggtgatga ttgaaaggat tatcagcggt
    32881 gacctgatgg tggccgcagc gctgtctggg atcttcccgg cggagcggca tggggatgat
    32941 gtgaagctgg ataccgccca cggcgaagcc tggcttcggt tcctgcgtaa ccaggaggag
    33001 aaggaaccgg ggcagcttaa cctttgcctc gcagacttcg tcgcagaaaa ggatgatcac
    33061 gccgggctct tcgtggtgtc ggtaaagacc gtcgatcagc tgccggcaga tattgcggga
    33121 gatgactacc gtaccataat gctgagaata ctcgctgacc gcttcgccga ggcggcagcc
    33181 gaatggctcc atcgcgagat acgcatgaaa cactggggct attcagcagg agagcagctc
    33241 actatggctg agatgttcaa ggtcaggtac cagggcataa ggccggcgcc gggatacccc
    33301 gcatgccccg accataccgg caagaaggtg ctgtttgata tactcagggt gccggagacg
    33361 ataggcgtca cgctgacaga gagttttgtg atggtgcctg tttcttcggt ttgcggttat
    33421 atatttgcat ctcccgggtc atcatacttc aacgtgggta caatcgggcg cgaccagctt
    33481 gaggactatg ccaacaggtg cggcatgacc gttgaggagg cggcaagatg gctggctccg
    33541 aatctttaga tttgcataat ggaacgaacg actgtaattg accttataag gaattcgaaa
    33601 aagccgctct tcacctttga gctgctgccg ccgctgaagg ggcacagcat tgagcgcata
    33661 tacaggacta ttgagtctct gcttgagtat gagccggcct atatcaactt cacctcgcac
    33721 cgcaatgaag aggtgctgac ggagagaccg gacgggcttc tggagaggcg tgtcacgcga
    33781 aaacgcccgg gaacaattgc acttgctgct gcggtgaaat acaagtacaa cgtgaacgtc
    33841 gtgcctcatg ttctgtgcgg cggattcaca aaggaggaga cggagaacgc ccttatagag
    33901 atgaattttc tgggaatcaa tgatgtgctg gcgctccgcg gcgatccgcc ccgtggcagc
    33961 cgtgtattca ttcccaacca gggcggccac cgaaacacca atgagctggt acagcagatt
    34021 gctgacatga accgcggaca ctacctcgat ccggacctta agaacagcgc agcaacgtca
    34081 ttctgcatcg gcgtggccgg atacccggag aagcatttcg aggctcccaa catgaccagt
    34141 gacatggcca accttaagcg taaggtggag gcgggggcga gctacattgt gacacagatg
    34201 ttttttgata atgccaagtt ctatgcgttc cgcgatgccc ttcgcgcaat gggggtgacg
    34261 gtaccgctga ttcccggcat caagccggta tcggcaatga ccgatgtgaa cctcctgcca
    34321 cagacatttc atattgacat gcctgaggag ctggtgcatc agctctcgca gtgccgcact
    34381 gatgatgagg cgagggaggt tggcatagag tgggctgcgg tgcagagccg cgacctcctt
    34441 aaaaacgaag tgccggggat acattactat accctcggca gatcggataa tattgcgcgg
    34501 attgtcaaag cctcattcta gaggccggca gatcccgttc agggatttat agtatttcta
    34561 cagcccgaat gccttcttta ttgagctgac cttgttgagt tcctcccacc tgaagagctg
    34621 gacagctacc ttcttgtcag gtttgccatt gaatgactta aaggttttct caacggttcg
    34681 cggcacgcgg cccatgtgtc cgtaggctgc agtctcgctg tagataggct tgcgcaggcc
    34741 atactccttc tcaatcattg ccggccggag gtcgaacatc ctatcaatga tctttgcgat
    34801 ctcaccgtcg ctctgcttca ccttcgaggt gccgaaggta ttcacgaaga tgccaaccgg
    34861 ttcggccacg ccgatggcat atgaaacctg gacaagcatt tcgcgggcaa cgccggcggc
    34921 aaccatgttc tttgctatat ggcgtgctgc gtatgctgcc gagcggtcga ccttcgaggg
    34981 gtccttgccc gagaatgcgc cgccgccgtg ggcgccgcgg ccgccatatg tgtcaacgat
    35041 gatctttcgg cccgtcaggc cgctgtcgcc gtgaggtccg ccgataacga acttgccggt
    35101 gggattgaca tgatatttga tttttccgtc aaagagctgc tgtacctctt tgccaaactg
    35161 cactttaacc cttggaacga ggatgttgat cacatccttt ttgatcgttt ccagcatctt
    35221 cttttcgtcc ttgtcaaaat catcatgctg ggtggaaacg actatggtgt gcactctctt
    35281 cggggtgccg ttgtcgttat attcaattgt gacctggctc tttgagtcgg gcctgagata
    35341 tttcatctgt ttgccttcgc ggcggatttc tgccagctgg tatacaagct catgggcaat
    35401 atcgagagag agagggagga agttttccgt ctcgtccacg gcgtagccga acatcattcc
    35461 ctggtcgcct gcaccctgtt cttccttctt cctgcgctcg acgcccatgg agatatcggg
    35521 cgactgttca tgtatagcag agaagattcc gcatgagttg ccgtcgaaca tgtactcggc
    35581 ctttgtgtag ccgatgcggt tgatgacttc gcggattatt gactgtacat ctacgtatgt
    35641 ttttgtcttg acctcgccgg caacaaccac ctggcctgtg gtgaccagtg tttcgcatgc
    35701 gacctttgat tcggggtccc atgccaggaa gttatcaaga agcgcgtcgg agatctggtc
    35761 ggctaccttg tcagggtgcc cttcggaaac ggattcagaa gtaaataaat agctcatctg
    35821 ttaatttttt acgtttaaca atggcaggga cggattgttg gcaaagcagc ggttggaaac
    35881 cgttttagca tttttttccg tggttgcaat cagtgcaaat ctgtccacct gtcgtataac
    35941 ggcacaaaga taatataatt tgcgatttgc gaattgcgaa ttgcgaattc ttgcaggtct
    36001 gcgacccacg gagatgtgat gcgggtcatt caagcgaatg agtcagcctg cagcgatggc
    36061 aataatggag tcggcactct tcagttatag aaatcatgca aaatgtatga tacaaaatgt
    36121 atgatacaaa atgtgtataa aatatatata tttgcaatat aaaacgacgt gtcatgactt
    36181 caaagaatcc gtttccggta gtcggatatc atggaaacaa gctgttttgc gacagagaga
    36241 cggaaaccgg gagactttta atgaatctca ccggaggatt gaatacaacc ctgatttccc
    36301 ttcgtagaat gggaaagact gcattaatat atcatgtttt tgataaaata ttaaaagata
    36361 tcagatctgt ttgtctctat gttgatctgt atcccacaag gaacatcagg gatcttacac
    36421 gtcagctggc gacagtttca atgaatgtac tgccacggaa aaaaagggca gggaatcgtt
    36481 ttcttacttt ccttaaagga ttaaggccgg ttattactta tgatcctctg acaggggctc
    36541 cgggggtgaa gtttgaatat tcatctgcct cggaatatga ggttacgctt agtgccctgc
    36601 tgaagtttct tgatgacctc gggactgaaa tttaccttgc attcgatgaa ttccagtcga
    36661 ttgcctcata cgaggagggg aatgccgagg ccatgctaag gactatgatt cagccgctga
    36721 aaaatattca tttcatattc agtggcagca acaagcatat gatgcatgag atctttcaca
    36781 gctcaaagag acctttcttt gcaagtacca gaattatgag cctgtcggct atctctgatg
    36841 atagttatag ccggtttatc ggggataagt ttgcagaaaa caaaagaaag gtatctcagg
    36901 aagcgatcag ttttgtactc gactggacca ggcggcatac atactatacc cagtctgtct
    36961 gcaatactct tttcagcacc ggggcgaaag aaattgatat tcggttagcg aaaacggtgt
    37021 gcggggaaat cctggatgaa caggaaaaga catttctgca ataccgccat ctgcttacat
    37081 ccaaccaatg gcagcttctc atggccgtgg cgaaggaggg gaaggtatat aagccccagt
    37141 caggggaatt ccttggcaaa cattccctcg gaacgccagc gggatcaaag agagctgttg
    37201 atgccctgct tgacaaggag atgatctatg tggagagtga cagcaacggg aagtattatt
    37261 ctgtttatga tctctttctc tcgaggtggc tggagaggca gtaggttttc ggcagtcaga
    37321 agtcaatgtg tttgttgccg gtctcaagcc tgaccgttgc cttgcctgca gcgatggcaa
    37381 tcgtaaggat ggcggttggg ctacaggaat tgcaggaatc cgcatacata ccgtgcccct
    37441 ctgtctccct ggctcggatt cagcaccagt agcaccacac cgcaaggcgt ggaatacgtt
    37501 gttccggatt ttactgaaga tcaggaacag tcaggtggca gacctgaccg ttcctgaagc
    37561 aattcagaat tgaaaggtaa ttggcaggtt gaagccatag aacgtgaaac tcgcccctgc
    37621 gcctcctgag tagatggcaa cgacggatgg attaatcccc agtttccaga ttcttatgcc
    37681 caggctgaca gagctgattg ctcccgggcc gtacgatgct ttcaggttat gctcgtcagc
    37741 aaacgggtcg cggctaatgt ttacaattcc tacatattcc ttgaaagaaa agaaccctat
    37801 tccaaactct gtggtaatgc tgaatatttt acctctgatc ccggggctga ctccaaaata
    37861 aacccctcct ccgttgatta tgtaatcatg attatccgca tactgcgggt ttacataacc
    37921 atactccaac aaatccaggc ctttcacaaa tattgatcca acggtaatat ccaggtattc
    37981 gagttttgtc gggatattta gtttaagact gatgcctccg atacttgcat ctccgttatt
    38041 agtggcattt ctatatttga atttgaaata gttatctgca ttcaggttat tgtaaagata
    38101 caaatacccg atttcaactc cgggtttacc attgctctcg tcaaaccatt tctcctggga
    38161 gaacgcaggg agtccggaca gaaacagggc aattagaatg actgtttgtt tacacatggc
    38221 taattaggtt taattatctg attcactaac acgaatttga acgccataat taggaaatac
    38281 cagccatcgt tcaggttggt cataatagtt caggtacgaa ttaccacatt tttccccatg
    38341 gtcttcaaaa gtaatttcaa aatcagatgc accagtggat tgcagacctt gttttttctc
    38401 gtccttctgg ttatatgcca ccgaaatttc aaaagtatat ttactggaag gcttatcata
    38461 ctcaaggaca gtgaatgtaa gcatatcata ccaaaaacca ggtttccatt taagtacctc
    38521 tttgccgtaa aagacctggg acacattagt cttgtctaca aattgacaat ctatttgttc
    38581 ctgaactgta tatggctctt tgcccgcatc aacattacta actgcaatat agaattcagg
    38641 cttgcctgcc ggccatcttt cgagtgtcca gtccgtaaat tttatctgtt tgatatagac
    38701 cggagactct tgtgatatat cccgatatgg aactttaaca aaatcatatt tggcgtttga
    38761 ttccccaaag gaggttacat gatttatttt gtaattaacc gttttcccgg ctattacttc
    38821 tgtgtcaatg tagttatgtt cattagggga gaaagattta taaaattgat atccaggagt
    38881 aactccgaca atatattttg aaagcctggt ttcctggata tattgagtgt gatcgttttc
    38941 ccatcttaac tcaatctcat tccttgtctg ttgaattgca tcaaatgatg ttggaggttt
    39001 agggtagttt ggggctgtta ttgaaatgta attagatgga tcagaagtaa gcccctggta
    39061 gtatgctgcc acataataag agtaggaacg acttgcttct acattattgt catcataact
    39121 tctgttatat gctccgtaca cattttgtgt cagactgtag gccggagatc ctgtggtttt
    39181 acggtaaatg tagtaaccag aagtgttcga tgggtcggta ccggcaggca ttgtccaggt
    39241 cagcctgatt ccggattctg tctgcgtccc gatcagattg gtcggagcag aaggctttac
    39301 cggattgtcc ggatcaggct caatgatcat tctttcattc tcactgatga caatgaccgg
    39361 ctcatcgggt tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttacctgt
    39481 ttgtctacga agtgtctcaa tagcacatca taatcgccat caattaattg aagcgcttca
    39541 tctctgacca tgtaacgaaa gtctttgtca gatttcaaag cctcactcac cacacgggcg
    39601 aaatcactga aatcagagtt gtactcattt tcctcataat ggccaactgg cttgacattt
    39661 tcattacttg ttgcctcctt catttcgcat gattggacaa cagtccacaa tacaagacct
    39721 gcaacaaaca gccagatatt gattccaatt gtttcttttc ccataattca caagttttaa
    39781 tctgcagcta aaagttatta aaccaaatac ttacattcag tcttactaat tgtattccac
    39841 acaatcaaag atggatgctc tcctttttag gggtgtcatt catccgtacg tgtcgcaatc
    39901 ctggattttg tgacatgaaa tttcaggata tttttatgca gggaactaat ttagacgttc
    39961 tccaaataat ataaataatg ctaaagaggc tctgtatcgt ggcccaatac tcttgttctc
    40021 taaatattga aaattcggtc taagatcttt ttctctcgaa gtggctggag aggcagcaga
    40081 gttcgatcgg cagccaatga attagttacc gggtacagtc cggagagtgg ccagggggag
    40141 ggggtgagtc tgcctttaca attcttgatc ctcttcgagg atctgtttat ataccgcagt
    40201 attacaattc tttatgctca atgagcattc tctggcatga tcgttctgct ggctgcgggc
    40261 tatttcccgg tcagttgtct gaatgtttcc tcgaggcggg cgccggcgag ggaggaaatc
    40321 tcgtccaagg ggccgtcagc cacaatacgg ccgtggtcta ttatgatcac cctgctgcag
    40381 atggcttcca cctcctgcat gatatgtgtc gacagcatca cggtcttttc tttgccggct
    40441 tcggagatga ggttacgtat ctccactatc tggttgggat caagcccggt ggtggcctcg
    40501 tcaagaatca gcaccttcgg atcgtgaatc agtgcctgtg ccagtcccac ccgctgccgg
    40561 taacccttgg agagggcgcc tatcttcttc ccctgttccg gggtgagtcc ggtgcgctca
    40621 ataacggaac tgacagcttc gcggctcttt ctgcccggcg aataaatgtc ggcaacatat
    40681 ccgaggtact ccttcacata catatcgagg tagagagggt tgttctcagg caggtagcct
    40741 atctgccgcc tcatctccct ggctgacgga ccggcgggga gtccgcatac cgtcacctgt
    40801 cccgcgtctg cgggaaagaa gccggtgata attttcatca tcgtcgactt tcctgcgccg
    40861 ttggggccaa tgaaacctac aatctcccct gccgggatgg tgaatgaaac atcatccagg
    40921 gccttttgcg gcccatagtt cttggtgacg ttttgaacaa caatggacat tgttgaattg
    40981 cgatttgcga attgcgaatt gcgattagcg attagcgatt tattcaggta ttaattcgaa
    41041 aatcgaaaat cgaaaatcgc caatttctag ccgtacatga actgaccgag gttggcaaat
    41101 gccgaggtcg ggttcacgca gcagacaggt atcttcgagc tgttggcgat gatgtactgc
    41161 tccttggcgc cccagacata gtcggcaaaa gtgatatcct tggtggtcat gatcagcac
//
LOCUS       DAOPXU010000010        39212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_907_length_39212_cov_13.791889, whole genome shotgun sequence.
ACCESSION   DAOPXU010000010 DAOPXU010000000
VERSION     DAOPXU010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 39212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39212
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_907_length_39212_cov_13.791889"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PKK03_01815"
     CDS             complement(<1..643)
                     /locus_tag="PKK03_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007760248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX83158.1"
                     /translation="MKILIVEDEPRVASFIKEGLEEKEYETVVAYDGITGEVAALKDE
                     VGLVILDLIIPGKSGMDVCRSVKAVRPDLPVLMLTALSTTVDKVRGFDAGADDYLVKP
                     FDFPELLARIKSLLKRSRTATHQTESLRVADLELDLMRKVAIRGGKVIELTAKEFALL
                     EYFMRNSGRVLSRVDISEKVWDITYDTGTNIVEVYVNILRKKIDRDFEPKLIHT"
     gene            835..1788
                     /locus_tag="PKK03_01820"
     CDS             835..1788
                     /locus_tag="PKK03_01820"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNX83159.1"
                     /translation="MTSAIILRQIGILAAIVVIGLIACRARVITAGTKDFLAKLIFYV
                     TLPSMLFTSFSAIEVTPRLLANSLQALLLAVMIMLFMLFAGWLTTVIFGIKGGHGSIF
                     RLHSMLGNIIYLGLPVISAQFGQEGVLYGSIFVLVSNILMWTLGVGIITPAGSFSLKS
                     GVRRIFNINTIAIISGFLLFLFSVKLPQFVLDTVGALGKTNTYLSMIFIAAVMYFADG
                     RKMLRNRNAYLLSFNRLLLVPFILLGLFILFNLLFPGILRKEVVSVMLMQAAMPCMVN
                     VVIMVNILGEDDGTATANVFVSTIFSVITLPLILLSLSLFE"
     gene            complement(1947..2510)
                     /locus_tag="PKK03_01825"
     CDS             complement(1947..2510)
                     /locus_tag="PKK03_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83160.1"
                     /translation="MKINSIRLQALSLIVMAALIIPQTLMAQGSKTNFSGSWTYNAAK
                     SSQPQGQPQGQPQGQGQRPGGPGGGGFGGGDFTVTQEGNLLTVARTMTGPDGTERTTT
                     SKYTLDGKESINTSRMGDSKSFATWSSDGKKLTIKTTRTMSRNGESMTMTSAEVWSLT
                     DGKTLQIETTMSAPNGERKQTSVYNKK"
     gene            2730..3584
                     /locus_tag="PKK03_01830"
     CDS             2730..3584
                     /locus_tag="PKK03_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83161.1"
                     /translation="MKNYLIAGMTALVILTSCEKIDRDAVFVINGKTEFTADDISLYD
                     TSAHILYLKRNHSEFEKIGRGTFAFYDKGEPVFSGEFWPGYLSSMPVGPVIMTAPLAL
                     QKHALKIEVWSRELSPELNNSRLTALLNRNNLLHSGLAVSSVSASVSGSELVFSFTLT
                     NMDQTDLLILDPAKTGPGLFRYFTNGLSLYNPENNNKVFADNIQHSTPEPWNSWSKEW
                     LTLLGAGESKEFTFSYTPGTPLQPGEYKASFEYPGLSYQVGLDELFQDGARVWLGEIG
                     INEIITIQ"
     gene            complement(3632..3808)
                     /locus_tag="PKK03_01835"
     CDS             complement(3632..3808)
                     /locus_tag="PKK03_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNX83162.1"
                     /translation="MDTKSLVLKTLNESGNPMKSAEIAAKAGIEKAEVDKVIKILKKE
                     DKITSPKNCYYSPK"
     gene            3977..4798
                     /locus_tag="PKK03_01840"
     CDS             3977..4798
                     /locus_tag="PKK03_01840"
                     /inference="COORDINATES: protein motif:HMM:NF037749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5602 domain-containing protein"
                     /protein_id="HNX83163.1"
                     /translation="MKRTHKLLALTLAAGVLSFLQACQKDDGFISRSTVDLKNGQAFV
                     NNANPERTFYSSARHIGNGRAQAWVTENREGEPVSVGLTLSAGALTNLPDEMTGFVLE
                     LPKGKGKNFYTFVMLDWNPQGHEPPQIYGLPHFDVHFYIVPDEVRLGMLPEKVTEFEN
                     LPSDEYVPAGYFRGPGFVPFMGVHWLNGASHELHGATFTQTFIWGSYDGEFVFWEPML
                     TRDYLLTRPNEVIGIPQPAAYKRDGWYPTRYEISWSKYGNDYTIALRDLQFRQGQ"
     gene            5070..6725
                     /locus_tag="PKK03_01845"
     CDS             5070..6725
                     /locus_tag="PKK03_01845"
                     /inference="COORDINATES: protein motif:HMM:NF003007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HNX83164.1"
                     /translation="MEVFKNLFTGSGITATILYLGITAFTGILLGRLEIRKLKLGVAG
                     VLFSGLLLAHFGVRTDLHVLTFVRDFGLILFVYAVGIDVGPRFFSSFRREGLKLNLFA
                     AGIVLTGFLTAFLLHLWRDVPPAVISGIMSGAVTNTPGLGAVQQVLTDMGRADDAAQA
                     GLGYAVAYPFGVLGVILAMVLYRYFFRIKINNEVEDFNRQYHQNRVKLESVEIHITNP
                     NIIGRKIRYVKQVVDRELVLSRILRKGTYMVAAEEIVLEDGDILYGVSSAEHADNLRM
                     TMGDVKFADKREITGDLAMFHVLVTNRKLAGKTIEQVGIYRRYEANITRIFRSGLEIL
                     PTRDTTIELGDTVRVVGKRDLLPDIRQELGNSLIELAHPNTVPLFLGIVLGILLGSIP
                     VFIPGLPAPVKLGMAGGPLIVALLFGYRGRIGKLDFYMTPGANMMLRELGIILFLACV
                     GLGSGAELVKTLVNGGYIWLLYGAAITFVPVALATIVGRCMKMNYLQLCGLVSGAMTD
                     PPALEFANSLAPVHAQSTAYATVYPLTMFLRIILAQTLLFLTL"
     gene            6795..7673
                     /locus_tag="PKK03_01850"
     CDS             6795..7673
                     /locus_tag="PKK03_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNX83165.1"
                     /translation="MVMRTIMDNIRTYVLMALGLLIFSVAWTAFLLPHQISGSGVSGL
                     AAIIFYATGIPMGYTYFAINVILILVGLKVLGAGFGIKTIYGIVLGAAMLSVLQPFIP
                     GPVVQEKFMSALIGGGLCGVGLAIIFTQGGSTGGTDIIAMVVTKYRNISLGRVILYCD
                     VVIIGSSYLILTEMEPMQRVETMVYGFVTMAIQAYTLDTILSGNRQSVQVFIFSRHFE
                     QIADQITGKLHRGATVIDGTGWYSKENQKVIITLVRKNEASDLYRIIRSIDDKAFITV
                     ANVMGVYGKGFEELKR"
     gene            complement(7722..7794)
                     /locus_tag="PKK03_01855"
     tRNA            complement(7722..7794)
                     /locus_tag="PKK03_01855"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7759..7761),aa:Gln,seq:ctg)
     gene            7959..8624
                     /locus_tag="PKK03_01860"
     CDS             7959..8624
                     /locus_tag="PKK03_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83166.1"
                     /translation="MKRMRMFLVVACTIAALQPASSQKLETDNLENLVFPKFQESVVR
                     LKNGQSYQAVLNYEKTERQMVVLRNNQLYLFKDHQAVDTIFMAGRIFVPAETGFYELL
                     VNGPVTLFVNHRATLDNTGATLAYGSKTTTAGVTHVNKIFSQDGAINLRIPENFKVAD
                     DTNFLLRVDGQIQKVLNKKQFLKLFPAKTRELEEFISSNNTSFKSQNDLISLVEFCNA
                     LNR"
     gene            complement(8650..10341)
                     /locus_tag="PKK03_01865"
     CDS             complement(8650..10341)
                     /locus_tag="PKK03_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNX83167.1"
                     /translation="MKESEEIIKPDSQHFIEQAIERDLGEGKNSGRVHTRFPPEPNGY
                     LHIGHAKAICLDFGMAEKYGGKCNLRFDDTNPTKEDVEYVDSIKEDIKWLGFTWEDRE
                     FYASDYFGRLWDFAVSLVKRGLAYVDDQSAETISEQKGTPTQPGAESPYRNRSIEENL
                     DLFYRMNDGEFEEGSRVLRARIDMASPNMHMRDPIIYRILKTPHHRTGTTWKVYPMYD
                     FAHGQSDYFEGITHSLCTLEFEVHRPLYDWFIDQLKTNDYRPRQIEFNRLNLTYTVMS
                     KRKLLELVKDGVVSGWDDPRMPTLCGLRRRGYTPESVRRFVDLIGYTKVEAINDVSLL
                     EYAIREDLNKRAPRVFGVLRPLKVVITNYPEDQVEQMEIDNNPEDEAHGKRLVPFSRE
                     IFIEKDDFMENPPNKFFRLAPGQEVRLKGAYIIKCTALLKDNNSEVNEVHCTYDPETR
                     SGGPQSDRKVKGTLHWVSAAHSLDAEVRLYDRLFSDEDPSGHKDIDPREFLNPGSLTI
                     IKGCKVEPSLADVRPLDKFQFQRIGYFCADYDSRPGSLVFNRTVGLKDTWQKINK"
     gene            complement(10516..10866)
                     /gene="folB"
                     /locus_tag="PKK03_01870"
     CDS             complement(10516..10866)
                     /gene="folB"
                     /locus_tag="PKK03_01870"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNX83168.1"
                     /translation="MGVIRIEDMEFYAFHGHYHEEQIVGSHFMVDVTIETDTDKAGKS
                     DELRDTLNYQTAYLIIKREMEKTSNLLEHIATRILEALCSELPGIRKAIVRVSKLNPV
                     MGGRIGRVSVEVSR"
     gene            11026..11622
                     /locus_tag="PKK03_01875"
     CDS             11026..11622
                     /locus_tag="PKK03_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83169.1"
                     /translation="MENEEKLMTGEESLRVISDMISKTRVSVTQASFHLLLWGWLIFA
                     CSLSEFLLWKYAGWADSWYVWFAVIAGVVASFAYGFAKGKRERIFTYGTKIYVWTWIA
                     FFWASVIFFIVFPLETGDVAKYMLLMAGIPLLISGVVLNFRPMMLGAAAFWLLALVAH
                     FGGDTVSGLAMPAAMIIGYLIPGYLLRKRGSHDTFQGA"
     gene            11600..11890
                     /locus_tag="PKK03_01880"
     CDS             11600..11890
                     /locus_tag="PKK03_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNX83170.1"
                     /translation="MTPFRELDPLLHSQLRLAIMSILISVDSADFIYLREKTDATAGN
                     LSVQIGKLKEAGYISVKKSFGESYPQTMCRITDLGRQRFAEYVAALKDYLRV"
     gene            complement(11926..13119)
                     /locus_tag="PKK03_01885"
     CDS             complement(11926..13119)
                     /locus_tag="PKK03_01885"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNX83171.1"
                     /translation="MTTRRSFIRQSGLTAGGLATATALGASVFPAGEGSFRRQEGMKD
                     LKISKMFAEPLSIEMEPFPIALGVMTHLDNAFVTIVLENGIEGYGEAAPIMTISGENQ
                     QTILGTLNSCREFIVGQDINNYRSIAYTLKSAFWAQSVARCAIEMALLDAYTKTLKIP
                     FYRFLGGTDNRIETDYTIAIVPAEQAKKEAIQLAAEGFRVIKTKVGVNLKEDVERVLA
                     IRDGAPECGILIDANQGYSPKTALRFINEVVNQGIFPVMFEQPVHKNDLAGMKYVRDN
                     TEILVGADESVFTRADAINIVRTGCADAINIKLMKSCIIESLDIAAIARSANLKLMIG
                     CMVETNLALGCAVHFAAGVGGFEFIDLDPSFNASECPVSGGPVYKAPLWTIGPEAGLG
                     FSKRK"
     gene            complement(13204..14943)
                     /locus_tag="PKK03_01890"
     CDS             complement(13204..14943)
                     /locus_tag="PKK03_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HNX83172.1"
                     /translation="MIFSKLNLILGTAVTVFLAGCGSNALIKARLEAPPATDAILVNQ
                     AGYLPDATKVALLRADADIFELIDVSTGRNVYGAEPGEPLYWELSGDTVRAADFSAFT
                     TPGTYRICLEGKDICSAPFRIGEDVYSEITRGAVRSYYLNRTGIEITEEFGGKWARPA
                     GHPDTAVIIHSSAASAGRPAGTVISSPGGWYDAGDYNKYIVNSSITNYTLLLFYQMFP
                     EYCRSLELNIPESGNKIPDLVDELLWNLRWYLTMQDPADGGVYHKLTNLAFGGFVMPH
                     EASEPRYVVMKSTAATLDFAAVTAMAYRVFSNDPSVELRALAATCLEASDKAMKWAVA
                     HPDVIYRQPDDVSTGAYGDGNLSDELFWAKAETALAQMEVPGEGFLDGVRAVTPTWNQ
                     SETLGLISLALSSDDSFAALREAAAALLTAYADELAGKSEKCPYRISLDRFAWGSTSD
                     VANQAMIKIVAMKITGSDRYLPSLQADLNWLLGTNPTGYCFVTGFGTLSPLHIHHRPS
                     GADGVPEPVPGFLVGGPNTVVMNDCQPPVERSTFPAKSYSDVECSYSTNEIAINWNAP
                     LVFLLGAMDSMRP"
     gene            complement(14948..17380)
                     /locus_tag="PKK03_01895"
     CDS             complement(14948..17380)
                     /locus_tag="PKK03_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNX83173.1"
                     /translation="MKFGHFDDAAKEYVITSYRTPWPWINYLGNKDFFSIISNTAGGY
                     SFYRDARLRRITRYRYNNVPADDGGKYFYINDNGTVWSPGWKPVKADVENYSCHHGMG
                     YTRIGSEKNGLRADMLFFVPLGDTCEVQRITLRNNGRMPKRIRLFSFVEWCLWNAHDD
                     QTNFQRNFSTGEVEVEDGVIYHKTEYRERRNHYAFYSVNRQVAGFDTDRETFLGLYNG
                     FDRPDTVFAGQPRNSIAHGWSPVASHCLVADLEPGEVKEYVFVLGYVENPDSEKFIAP
                     KVINKARAKAMTGRYDTVEKVDAAFAALMEYWKSQLSVITLTHPDPRLERMVNTWNPY
                     QCMATYNFSRSASYFESGIGRGMGFRDSNQDLAGYVQMIAPQARERIIDIASTQLPDG
                     SAYHQYQPLTRRGNAEIGGNFNDDPLWLIFGVATYLKETGDWSILDETVPFNNNPDDA
                     ASLFEHLRRSFMHVVKNLGPHGLPLIGRADWNDCLNLNCFSSNPDESFQTTENQKGGV
                     AESLFIAAMFVLYGADFVTFARRRGEAQMADDAARQIEGMVKAINAHGWDGEWFLRAY
                     DFYGRKVGSRENEEGKIFIESQGMCIMAGIGLDDGRALKALASVNKHLACEYGIVLNN
                     PAFTKYLLNLGEISSYPEGYKENAGVFCHNNPWIIIAETITGNGHRAFDYYSRITPAY
                     LEEISDLHKTEPYVYSQMIAGKDAFRPGEAKNSWLTGTAAWCYYAVTQYILGIRPDYD
                     GLMVDPCIPADWGGFTVKRRFRGAVYNIEVINPERKSKGVWEVTVDGKKVDSCILPVF
                     PEGTEHNVKIKM"
     gene            complement(17459..19333)
                     /locus_tag="PKK03_01900"
     CDS             complement(17459..19333)
                     /locus_tag="PKK03_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HNX83174.1"
                     /translation="MNLHIIDLGIVILYLLATIFIGNWVSKRASRNIQSYFLGGNSLP
                     WYFLGVSNASGMFDIAGTMLLVYWLSVYGLKSLWIPWLWPVFNQIVLMVFLSAWLRRS
                     NVMTGAEWIKTRFGEGRAANLSHIIVVIFAIVSVIGFLSYGFKGVGKFVAAFLPPLVS
                     NPEYLVTHPQVNVNLYALILMAITTFYVVKGGMFSVVITEVIQYGILTIASIAVGIIA
                     ISQISPDALNSVIPEGWKTMWFGRTTGLDWHTISSAASPKLHSFNDWIKTDGYELFGL
                     FFVMMLFKGILVSAAGPAPNYDMQRILATKNPKEASKMSSLVSVVLNPTRYFMVAGLT
                     ILALVNFDKLYTGSLTNPDFESILPEVLATYVPVGLLGFLLAGLIAAFMSNFAATVNA
                     APAYLVNDIYKRYVNPHASEKTYVRMSYAASLLIVVIGIAVGFLVTSINDVVLWITAA
                     LWGGYTAPNFLKWYWWRFNGYGYFWGMLSGIAAALLLPALNLDLLQNWPLTENFSMNA
                     FPVIFLISLVGSILGSLLTKKEDDETLKRFYRQVRPWGFWKPVEKMVLAEDPSFKPNR
                     DFWRDMFNIAVGIVWQLSLMAFPVFLVIREWKQFYVAVAVMIVTTVILKFTWWDKLKD
                     "
     gene            complement(19361..20107)
                     /locus_tag="PKK03_01905"
     CDS             complement(19361..20107)
                     /locus_tag="PKK03_01905"
                     /inference="COORDINATES: protein motif:HMM:NF040269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutT"
                     /protein_id="HNX83175.1"
                     /translation="MISHKIVPNISVDCVVFGFDFEKLNVLLVKREMVDKKSGKVLIL
                     DHTLAGYHIFEDEDLDSAATRILKGLTGLDNIFLEQFGAFGQLDRVSSPKDKMWLEYI
                     NQGFADRIVTVGYLALIDNTKVTLTLKDRDVSWFPVSMLAEMEMAFDHKYLFYKALEA
                     LQRKVKVEPIAFELLPDYFTLSQLQKLYEALLGTSFDKRNFRKKVAQMHYVIPLKKKQ
                     QGVPHKPAQFYLFSREVYEKTSKGRISFII"
     gene            20530..23637
                     /locus_tag="PKK03_01910"
     CDS             20530..23637
                     /locus_tag="PKK03_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX83176.1"
                     /translation="MSTTMLFAQQRTVTGRVVDDNNAALPGASVVVKGTTIGTATDLN
                     GNFTLPVPSDATTLVITFVGMDAKEVPITAGPINVTLTMSAEEVDAVVVIGYGTIRRS
                     EVTSAIASVKTEEIQDLVVTGVDQALQGKVAGLQVMNNSGQPGGGVSVRVRGITSINS
                     NDPLIVIDGVPFTNNNVENTGYDGLGGSDGQTQNSFLATLNPNDIESIDVLKDASAQA
                     IYGSQAANGVILITTKKGKAGDGKVTYDFSYGLQQVGRTLDVMNLREFAEYQNSVIAE
                     LGSGYIPTEEFADPSLLGEGTDWQDAIFRQGYTMDHQLGISGGKENTSYYFSAGYFDQ
                     KGILIGSDFKRYSTRFNLDSQVKKWLRAGISSNITRAIQNVTLADAAESTIWWATLQN
                     PLIPVRNLDGTWAGNYTVGGYTYTADNPVATSNSRGNKGVNSQIFGNIYADVIFFEGL
                     SLRNEFSYQLGLQNNMAFQYEANIGTRSLQAQLYDSRNNSYYYAVRNYLNYNKNLGKH
                     KLAFTGGHEAQYSYWENMGGKKVDLQNNILDMNAGGTDKLTWDLTGGKGDWAMESYFA
                     RGNYTFDDRYSLSLSYRGDKSSNFGPNKKWGFFPGASVGWTVTNEKFAANWANVMNYM
                     KIRLGAGAVGNQNLPGGAPNPPYTSNVNFWPGPTGFGQVGTASTNFIAGIANPDLGWE
                     SVITYNGGIDFGFLNGKIDLTVDLYKKVTSNMLLFSTGPALLGIGDAWNDLKAPIGNV
                     GQMTNTGIDISLVTRNISKPNFSWNTSFIFSHYKNVLDKLINESSSIDGKLYYDNYLI
                     THTVPGYAVGTFWGLVTDGLFRTETDLANSFPQFGSAVSQNETWLGDVRYKDVHKDQV
                     IDSKDLTFIGSPIPDFTFGLTNTFRYRDLDLSIFLQGSYGAEIFNFMKWQLERMNNVY
                     YNQSTAVMDRYTATNKDGALPRFTNTNTNNTAMSDRYVEDGSYLRIQNITLGYRLPGT
                     VINRAQMSNLRLFVTVQNLYTFTNYTGYDPEVGSYNNSIRLMNVDAGHYPNPRTYMGG
                     VSVEF"
     gene            23657..25261
                     /locus_tag="PKK03_01915"
     CDS             23657..25261
                     /locus_tag="PKK03_01915"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX83177.1"
                     /translation="MKNRFIYFIVILAATLASCSESFTDRPSLSGATVSNYYNTADEV
                     RAATSTIYSGLAWSGYEARALDCIGEILAGNEHTYNGDDTPFQLMTVSATSVRLSDSW
                     KAFYKVNGWTSALIATFEQKIADGGDAAILEPAIAECHFLRGTVYFYIARIWGHAPIV
                     TDPGAIALTGDFNIPRYFQEDVLRFALEELQLAEAGLPETDVPGRVTKYSAKGMMAKL
                     YLYQKDYANAKAKAWEVMESGQYDLFPDYRAMFNSSDNNNNIETLFAVQHQCTMNPWG
                     SGNQLNPDRGPSSLQTDEASMWELYRPSMDLRTAYEPNDKRRAGSMMEHGWNMADWKP
                     QKLPQADGSFSPDDAAYNDFMKDGFRYDTIQDVKQGGTLNGTRSTIAKYVVGPGQKYG
                     GEQVIGMNTGINFMMQRYADILLIYAEATLGEAASTTDATALEAFNKVRLRAGLPVKE
                     VLTLNDIMKERRVEFAFEGDYWFDITRQGFEKAKQIIEAQNRGTISGIVRVTGFTEDK
                     MFLPIPASEVLQDPLLNEDPQPYYTK"
     gene            25273..26502
                     /locus_tag="PKK03_01920"
     CDS             25273..26502
                     /locus_tag="PKK03_01920"
                     /inference="COORDINATES: protein motif:HMM:NF037325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycan-binding surface protein"
                     /protein_id="HNX83178.1"
                     /translation="MKKIINNTLSALLLLLLAGAAGMVFQACEGNEEDMPMPEIFYIR
                     KADPAKSDSLVAHAFMGDNIAIIGRNLGNVDEVWFNDQKAGLNPNLVTPTTIIVSVPQ
                     VIPSTVTNYMYLINMNKKDTLKYTFGVDVPAPLVDRMVCEYVADGETAVIKGNYFVDD
                     PASPLTVLFPGNVPGTIVSFTINEIHVTVPSGAGPGQIQVKSLYGTTRSRFFFRDDRN
                     IILNFDDLTAAGGWRSGVMGNSNPEGISGNYVRFSGALAGGAGVTWNEDGFSFNLWPQ
                     ANGRPDVPVYTGELKDAEIKFEINVVEAWESCALQMIFTPYSVTGANSYIADGSVPRG
                     VWNPWKATGTYKTAGWETVSYPLSEFNYSFDGSVSANKLTADMMRGLTFYVWHGGVAG
                     KDCNPHICIDNIRVVPK"
     gene            26526..28664
                     /locus_tag="PKK03_01925"
     CDS             26526..28664
                     /locus_tag="PKK03_01925"
                     /inference="COORDINATES: protein motif:HMM:NF037325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycan-binding surface protein"
                     /protein_id="HNX83179.1"
                     /translation="MHKIQIKFRNWALLFATICVVGAGLLFTSCEGKEEEDTKVVLQS
                     FGPMPIARGAELKFIGLNLDKVTAVILPDNITISTFTKKEARLLTLTVPQEAVPGYVV
                     LKTPDGDITTKTTIGFSEPISIAGLTPATVKAGDELTITGDYLNLVEEVILTDRVTVT
                     EDDFTSHTRTEIKLVVPAEAQTGKIAVSNGAEEPIIVYSASTLTVTLPAFTTVTPNPV
                     KAGTNLTIAGTNLDLVLSVKLGGGKVIEAANFVSRTATQIVLAVPADTKDGKVILVPA
                     SGVEVSTATDLVMVVPTVSVTPVTLKNGQDITVTGTNLDLVDHVIFGGDKQGTIKAGG
                     TATQILVTVPDDAVDGVVTFVTKADKEIAGPNLTMIVPAFSSFSPTSARANSTVTLSG
                     TDLDLVTKVIFTGGLEGSIGTRTGTSLAVTVPVGAKTGKITIVTKNGTQVVSAIDFTL
                     LANLPTFGSYTETRGEPGKILTINGTNMLLVKELIFPGEKKATAYGSKSDTRIEVYVP
                     EDVTFGTGTIRLVTYEGEEGFFPELYFGGTDPVLNQTFCFFDFNGTGKDSWWGNAIGS
                     GILNDPASSADGTPYWNINGMGGSGWWDGLFFRNGSNNFVTTGVDVNTWAVRFDINVR
                     EAITEGDLRIRLGSYFYHFQPWNGVAGGFKTTGWITVTCPLTGFKTDDGTASIPDAAL
                     GGSEFGMIWSHGVSVKVNMGIDNVRFEPIN"
     gene            28765..32031
                     /locus_tag="PKK03_01930"
     CDS             28765..32031
                     /locus_tag="PKK03_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNX83180.1"
                     /translation="MRLFTRLVLPVVLLLSGAGPAPAQGTKYEAENGTLTGSVSVQTT
                     VAGFSGTGYAGIFENDGDKAAVTFNLSAAGNYDLYIGYAGPYGDKKNILTINSNSIEA
                     SFPASPAFTETACGRVRLKEGSNTISITKSWGWFLLDYFRIEPDTKPDVSVLLPYKLS
                     TQAPHRETRMLWSYLMDSFTHRIHSGAMSLNAKEEAEWLFAQTGRYPALTGLDFMNHT
                     RNYSWYDKSVVVNEARNWYNRNGLVAICWHWRDPSRATEEFYTSGTSFDISKITETTS
                     AEYQAMLSDIDIIAGYLKQLNADKIPVLFRPLHEASGRWFWWGAKGPEPCKALWRLMF
                     DRLVNYHGLKNLIWVWTTDAAPDNLEWYPGDEYVDILGADIYAADGDFSSQLLTYDAI
                     KEKFGGRKLVTLSENGPVPDPERLMADGAHWSWFMPWYGSFIRDGIKNPLSHWQKVMS
                     HDYVVTLDEMPDLKNYPLSDEPDYSNFPQGFFMNGWKPRTAVVPDYTDVPAVTDPVTV
                     AVTVDCSDTVTLVSPYLFGDNANLWTGPMSDNATLMKNITNRDQGVMRGPGGSTSDAF
                     FWNRNTRPPDVPETLLNDPTNKSWPWYGQRAENWTMHVDSFYSILSKANITGMLTVNY
                     GYARYGTSADPVAQAAHMAADWVRYDRGRTKFWEIGNEVYGNWEAGYRIDRSLNKDGQ
                     PEYITPQLYGQHCRVFIDSMRAAAAETGFDIKIGVVMVEAASTHSSWNTGVASRVGDL
                     ADFYIVHSYYTPWNTDSDVATVLNSYKNTEGYKNYVWNAVAAAGMPQLPVALTEYNIF
                     AVGSNQPVSHANGMQAVLVTGEMIRTGYGAACRWDLANGWDNGNDHGMYSYNEPFIPN
                     YTPHPAFYHLYYMRRHTGDVLLRSTVTGAQGVVITPTAFSSGHLGASIVNTTKVHKVV
                     RLNLKDYGVGDRFYTYTLTGTEGQDFSRKVFVNGTGGTLTAGGPDSYETIRADAVVIG
                     DEIRINLPPLSAVYILVEPGTRQLAISNEVTSADPVRSDDSVTIWPNPSEGNFTVTGM
                     PDHVSRMEISDLRGNLLLSRETGRGEHEVTLDTDFVSGIYLVTLFGDNYTVTRKLIIK
                     "
     gene            32047..34899
                     /locus_tag="PKK03_01935"
     CDS             32047..34899
                     /locus_tag="PKK03_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNX83181.1"
                     /translation="MRRSLTVLFTVTALIAALANQSCSRTPYERTDNGVIIRLNGKSD
                     HPGQAVRLVVINERIIRVSAVPSGEFPETASLMAVAQPDDNMDFTIEKGDGLVTISTS
                     SLSAEVSLKTGGVVFTDNDGTVFLSELDGSGRSFMPVMAIGEKGYTVRQQFESYPDEA
                     IYGLGANQTSFMNLKGKDADLFQYNTLAVVPFIISNRNYGILWDNNSRTKYGDIRDWN
                     ELSSLKLYDSQGAENGLTATYADRKTGKKVFTTRNEREISYQFIPDLAEFPEGFNLGD
                     GKVIWEGAFEADTTGIFKFMFNSAGYARLWIDGRLLFDRWRQCWNASTNYFTLNVEQG
                     RRYPLKIEWIPDGGESYIALRYLTPQDGDEQQRISFWSEVADQIDYYFISGENMDEVI
                     RGYRTVTGKAPVMPKWAMGFWQSRQRYTNQEELLGVVREYRKRNIPFDNIVLDWQYWP
                     IDKWGDHQFDATRFPDPDGMIRTLHDDLNARIMISVWPKYYVGTGNYDAMKSKGYLYM
                     RNVEKERKDWIGYLSTFYDAFNADARAAFWDQINNALYSKGIDAWWLDATEPDITSNL
                     PMDERKAMMSPTALGSADRYFNAYSLVQAQGVFEGQRATDPENRVFILTRSAFAGLQR
                     YSAANWSGDIAARWHDMAAQIPCGLNFSMSGIPWWTMDIGGFSVESRYHNPSPADLDE
                     WRELMTRWHQYGAFVPLFRSHGEFPFREMYNTAPENHIAYKTMVEYNQLRYRFMPYIY
                     SLAGQAWLNDYTIMRGLTMDFSLDTAVFDIADQYMFGPSLMVNPVTKYKARSRKVYLP
                     SAYGWYDVRTGRHYAGGTVAEADAPYEWMPLFAREGSVIPTGPEIQYAEEKEADPLTL
                     WVYAGADGAFVLYEDEGDNYNYEDGAYTLIPFTWSEAERTLTIGRREGEYQGMLHERT
                     INVIMVSEYKAVKLDFGRTPDKSVTYSGEEIKVKFE"
     gene            34950..36683
                     /locus_tag="PKK03_01940"
     CDS             34950..36683
                     /locus_tag="PKK03_01940"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNX83182.1"
                     /translation="MKKVITVMTAMLLFAATVAAQSIVPAEWKFLTGDKPEYIDSAKN
                     EYWWKDISPLTVWERQGFPGYDGFAWYRVKVVVPASMKKNAEKYDGLMLNLGKIDDAD
                     ETFFNGHKVGATGKMPPDYAGAYDVPRTYLIPPDYVNWGGKNTIAVRVYDAGGDGGLY
                     GGPAELTNRGNPDLLTLGTLFAQEDMILKGSPDVEILTTVKSEFRKKYRGALTLKIVT
                     DFGEQVYDGYHDVAVKKMSSGNFPFLVKNLKPGFYKATLALQSKMGNKKYSFNFGYEP
                     EKIVSPADPQPDFAEFWQRAKAELATVDPRYNLIRIDSLCTEKHDVYLVEMRSLGNAL
                     IRGWYRVPVKPGRYPAIMQVPGYSSVEVPSYVNYGDDIIGFALNIRGHGNSKDDVNPG
                     FPGYILSNLGDKEKYIYRGAYMDCVRGIDFLCSRPEVDASRIAVEGASQGGALTFATA
                     ALANDRIAVCAPQVPFLSDFRDYFRVANWPGNEFIDLVENKKALTWDQAYYTLSYFDI
                     KNLAPMIKAPLIMGAGLMDEVCPPHINFAAFNLVTAEKKYIVYPYAGHGLPDHFYQAK
                     MDWIRTKLGLN"
     gene            36696..39125
                     /gene="galB"
                     /locus_tag="PKK03_01945"
     CDS             36696..39125
                     /gene="galB"
                     /locus_tag="PKK03_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase GalB"
                     /protein_id="HNX83183.1"
                     /translation="MEGRTKMSGLLIAVGVAILLQGCSQPVPRTHVSFNDGWKFALAD
                     AADASQPGFDDSGWRNLDLPHDWSIEGEFSEANPASPGGGALPGGVGWYRKTFRVNTA
                     DKGRMTYISFDGVYRNSEVWINGNYLGKRPYGYSSFRYDLTQWLKYGEEENVIAVRAD
                     NSAQPNSRWYSGSGIYRNVWLTTTEKIAVDQWGTFVTTPEVSSESALIRVTTQIKNST
                     GSKAEVRLETTVYDSDNKRVVRTETDQVEVPVSGTAVTQELTVRSPRLWSVDNPQLYR
                     VVTTIRSGQLLADRYETVTGIRSFEFDRVKGFILNGEPVVIRGVCNHHDLGCLGAAVN
                     TRAMERQLEILKEMGCNGIRTSHNPPAPELLDLCDRMGFIVMDEAFDIWKVKKTDFDY
                     SLDFDEWHRRDIEAMVLRDRNHPSVFIWSIGNEVMEQWERDGSGEAIATELAGIVRSL
                     DDTRPVTAACNDPVPYNPVIASGALDLIGYNYRDTLWTRFPQAFPEGKFIGTETTSAL
                     ATRGSYDMPSEIVRRWPSRWDQPFRDGNADLTCSAYDNCSAPWGTTHRDSWKLIRDNA
                     FLTGMYIWTGFDYLGEPTPYWWPARSSYFGIIDLAGFPKDVYWFYQSEWTDKDVLHVF
                     PHWNWNPGQTVDVWAFTNCDEVELFLNGSSLGRQSRSCDDFNLVWKVPFEEGTLLGVG
                     YRNGSEIMRSEIRTAGEAAALQLSPDRAEIMADGSDLSFITVTVADKDGNPLPHASNL
                     INFSVGGPGTIAGVDNGSQTSMEPFKADYRKAFNGKCLVVVKAGKEKGTIKLTASSEG
                     LPDATVTMRAR"
BASE COUNT         9579 a         9971 c        10266 g         9396 t
ORIGIN      
        1 gggtgtggat gagcttcggc tcgaagtccc tgtctatctt cttcctgagg atgttaacat
       61 agacctccac gatattggtt ccggtatcat aggttatgtc ccacactttt tccgaaatgt
      121 caacccgtga gaggaccctt ccgctgttgc gcatgaagta ttcaagcaga gcgaattcct
      181 tcgcggtaag ctcaatgact ttgccaccgc ggattgccac cttcctcatg aggtcaagct
      241 ccaggtcagc cacacgcagt gactcggtct ggtgcgttgc agtcctgctt cttttgagca
      301 gcgatttgat acgtgccagg agctccggga agtcaaaagg cttaacaagg taatcatcag
      361 cgcctgcgtc aaagcccctg accttgtcaa cggtggtgct gagcgccgta agcatcagca
      421 ccggaaggtc aggccggact gccttaaccg accggcagac gtccatgccg cttttgccag
      481 ggatgataag gtcaaggatc accaggccaa cctcatcttt cagggcagct acctctcccg
      541 tgataccatc gtatgccaca accgtttcat actccttctc ctcgaggcct tcctttatga
      601 aagaggccac cctgggttcg tcttcaacta tgagtatctt catggggcac cggtgatttt
      661 ggtctctggc ttgctcagcc tggtggttta ttatagcaat cctgcagccc aaaattagta
      721 aaactatttc atggagaaag tgtgacgggg actcttccgt tttcttccgg gagttatctt
      781 tgctgtggtt ttttttgcag accgccgctt aaagtaacat tatacaggat cttaatgacc
      841 tccgccatca tactccgaca gataggcata ctggcagcca tagttgttat cgggctgatt
      901 gcctgccgtg caagggtgat cactgcaggc acaaaggatt tccttgcgaa gctgatcttt
      961 tacgtgacgc ttccatccat gctctttacc agtttcagcg ccatcgaggt caccccgcgg
     1021 ctcctggcca acagcctgca ggcattgctc cttgcggtga tgatcatgct cttcatgctc
     1081 ttcgccggat ggctgactac cgtgatcttc ggaataaagg gggggcatgg ctccatattc
     1141 aggctccaca gcatgcttgg caacataatc tacctgggat tgcctgtgat ctctgcccag
     1201 ttcgggcagg agggagtgct ttacggcagc atcttcgtcc tggtttcaaa catcctgatg
     1261 tggacgctcg gggttggcat tataaccccg gcgggatcat tcagtctgaa atcaggagtt
     1321 agaagaatat ttaatatcaa tacgatagca attatttcag gattcctgct ctttctcttt
     1381 tcagtgaagc ttccgcagtt tgtccttgat acagttggtg cacttggaaa gaccaacacc
     1441 tacctttcaa tgattttcat cgccgcggta atgtattttg ctgatggcag gaagatgctc
     1501 cgcaacagaa atgcatacct gctctcgttc aacaggctgc tgctggtccc atttatcctt
     1561 ctgggattgt tcattctttt caacctgctg tttccgggca tccttcgcaa ggaggtggtc
     1621 tcggtcatgc tgatgcaggc ggcgatgccc tgcatggtca acgtggtaat aatggtgaac
     1681 atactgggtg aggatgacgg cacggcaaca gccaatgtat ttgtatcaac cattttcagc
     1741 gtaataacgc tgccgctcat actgctgagc ctgagtctgt tcgaataatg tttgcgggca
     1801 atggccccgc gaaccacgcc tgatttatgg cgggcagtca catatgcaaa acagggggat
     1861 tgtcaaaatc cccctgcagg ttatgcgggt atgttcattt tattctgcaa gccctgaacc
     1921 ctgaacccgg cctcgcagat gagaaattac ttcttattgt agactgaagt ctgctttctc
     1981 tccccgttgg gagcagacat ggttgtctct atctggagtg ttttgccgtc agtcaggctc
     2041 catacctcag cagaagtcat cgtcattgat tcaccgttcc tggacatggt cctggtggtt
     2101 tttattgtca gcttctttcc gtcagatgac catgtggcaa aagacttgct gtcgcccatg
     2161 cgtgaggtgt tgatactttc tttgccgtca agggtgtatt ttgaggttgt ggtcctttct
     2221 gtaccgtcag ggccggtcat ggttcttgca acggtaagga gattgccttc ctgggtgacg
     2281 gtaaagtcgc cgccgccgaa accaccgccg ccagggcctc cgggacgctg tccctgtccc
     2341 tgaggttgcc cctgaggctg tccctgtggc tggctgctct ttgcggcatt atatgtccag
     2401 ctgccgctga aatttgtctt cgacccctgg gccatcaatg tctggggaat gataagtgct
     2461 gccattacta tcaatgacag agcctgaagc ctgattgaat ttattttcat aattgtcctt
     2521 ttttgcggaa taaatccgga tgtttaacag tcaaaggtat tgcagttaaa gttaagggga
     2581 agttaaattg aaatttattt tttcacccgg gctgtgatca ggtcctgatg acttataacg
     2641 gaagcaagag ggtaattatc aaagccctga caggtctgct ggcatggtat ttgtgcgata
     2701 tttcctgtct aaccaattcc agcctggcca tgaagaacta cctcattgca ggaatgacag
     2761 ctcttgtcat cctgacttcg tgtgaaaaaa ttgacaggga tgccgttttc gttataaacg
     2821 gtaaaactga gtttacggct gatgacataa gtctttatga tacctcggca catatccttt
     2881 acctgaagcg gaatcacagt gaatttgaaa agataggaag aggcaccttc gccttttacg
     2941 ataagggtga acctgttttc tcaggagaat tctggcctgg ttatctcagt tcaatgccgg
     3001 tggggcctgt tatcatgact gctcccctgg cgcttcagaa acatgcgctg aaaattgaag
     3061 tctggtcacg tgagcttagc ccggaactga ataacagcag gctgacagct ctcctgaacc
     3121 gcaacaatct tttgcattcc gggttggccg tatcttcagt ttcggcatcc gtttccggat
     3181 cagaactggt tttcagcttt accctgacca acatggatca gactgatctg cttatacttg
     3241 atccggcgaa aaccggccca ggcctgttca ggtacttcac caacggactg tctctctata
     3301 accctgaaaa caataacaag gtctttgcag ataacattca gcattcgact cctgagccgt
     3361 ggaacagctg gagcaaagaa tggctgacac tcctcggtgc gggagaatcg aaggaattca
     3421 ccttttcata tacacccgga acgccattgc aacccggcga gtacaaggcg tcatttgaat
     3481 acccgggcct ttcataccag gtggggcttg acgagctctt ccaggatgga gccagggtat
     3541 ggctcggaga gatcggaatc aatgagatca taacaattca gtaatcccgc tttcccgccg
     3601 tctccggagg attttgcgga tcgtttcctt tttatttggg agaataataa cagtttttcg
     3661 gtgaggtgat tttatcttcc tttttcagga ttttgatcac cttgtcaacc tcagcttttt
     3721 ctatgcctgc ctttgctgca atttctgcag atttcatcgg atttccggat tcattcagtg
     3781 tcttcagtac cagtgatttc gtatccattt tgatgcggtg ttagtacggt tccaaaatta
     3841 agtgaacatt tctgaattca atttgttttt tctaatcaga aacatgtttc agcttcattg
     3901 tgtaaccggg aaaccacatc aaaaccgggg cagaactgaa aagccataag agtaggaatc
     3961 atttaaattt ccagttatga aaagaactca taaactactg gcgcttacgc tggcagccgg
     4021 agtgctctct tttcttcagg cttgtcagaa ggacgacgga tttatatccc ggtccacagt
     4081 tgatcttaaa aacgggcagg catttgtaaa taatgccaac cctgaacgaa ccttttacag
     4141 cagtgcccgt cacatcggaa acgggcgggc acaggcatgg gtaactgaaa acagggaggg
     4201 tgaacccgtc tctgttggtc tgactctctc agccggagcc cttaccaacc ttccggatga
     4261 aatgacaggg tttgtgcttg agcttccgaa aggaaagggc aagaacttct acacatttgt
     4321 gatgctcgac tggaatccgc agggacatga gccgcctcag atttacggat tgcctcactt
     4381 tgacgtccac ttttacattg ttccggatga ggtacggctg ggaatgcttc ccgagaaggt
     4441 gacggagttt gagaatcttc cgtctgatga atatgtcccg gccggatatt tccgcggacc
     4501 gggcttcgtt ccgtttatgg gtgttcactg gcttaacggg gcatctcacg agcttcatgg
     4561 tgccactttt acccagacat tcatatgggg ctcatacgac ggggagtttg ttttctggga
     4621 gccgatgctg accagggatt acctgctgac ccgtccgaat gaggtaattg gcattccgca
     4681 gcctgctgca tacaagcgtg acggctggta tcccacccga tatgagatct cctggtcaaa
     4741 atacgggaat gattacacca ttgccctgcg tgaccttcag ttccgccagg gccagtgatg
     4801 tatatccgct gacaggtttc gttttattca actccccggc tttttccacg ccggggagtt
     4861 tgaattcaca gacatataat tagccggcat agacaggaag tcttgtaagc atgactctct
     4921 gcccaggaga ttgctcttca cggaaagaat ggcatgacga gaatcagtca ttgactcctt
     4981 aacagttact atatttgggg ccgatgatta aaggtgccgc atccctgaaa ggagcggagt
     5041 accgtatagt atgaacgtga tgcattccta tggaggtatt taaaaacctg tttaccggat
     5101 cggggattac tgcaactata ctctatctcg gcattaccgc ctttacggga atactgctgg
     5161 gaagactgga aatccgaaaa ctaaaactgg gggtcgcagg ggttttgttc tccggactgc
     5221 tgctggcaca cttcggagta aggactgacc tgcatgtgct gacgtttgtc cgcgatttcg
     5281 gcctgatact gtttgtttat gccgtcggaa ttgatgtcgg accaaggttc ttcagcagct
     5341 tccgccggga gggactaaaa ctcaacctct tcgcggcagg gatagtgctg accggatttc
     5401 tgactgcctt tctgctacat ctgtggcggg atgtaccgcc ggcagtgatc tcaggaatta
     5461 tgagcggcgc tgtcaccaat acgcccggac tgggagccgt gcagcaggtg ctgaccgaca
     5521 tgggccgtgc tgatgatgca gcgcaggccg gactgggtta tgcagtggca tatcctttcg
     5581 gagtcctggg ggtgattctg gcaatggttc tctaccgtta ttttttccgc ataaagatta
     5641 ataatgaggt tgaggacttc aaccggcagt accaccagaa ccgggtgaaa cttgagagcg
     5701 ttgagataca cattaccaac ccgaatatca tcggccggaa gatcaggtat gtcaaacagg
     5761 ttgtcgacag ggaactggtc ctttcccgca tactgcgaaa aggaacatat atggttgctg
     5821 ctgaggagat tgtgcttgag gatggagaca tcctgtacgg ggtgtcatca gccgaacatg
     5881 ccgataacct gcgcatgacc atgggcgatg tgaagtttgc agacaagcgt gagatcacgg
     5941 gtgaccttgc aatgttccat gtacttgtca ccaacaggaa gctggcaggg aagacaattg
     6001 aacaggttgg catctacagg aggtatgagg ccaatataac ccgtatattc agatccgggc
     6061 tcgagatatt gcccacccgt gacaccacca ttgagctggg agataccgta cgagtggtag
     6121 gcaagcgtga cctgctgcca gatatacgcc aggaacttgg caactccctg attgaactgg
     6181 cgcatccgaa tactgttccc cttttcctcg ggattgtcct gggaatcctc ctgggaagca
     6241 ttccggtttt tatccccggt cttccggctc cggtcaagct gggtatggcg ggcggacctt
     6301 taatcgtcgc actgctattc gggtacaggg gaaggatagg aaagctcgat ttttacatga
     6361 ctcccggcgc gaacatgatg ctgagggaac tgggcataat tctcttcttg gcttgtgtgg
     6421 gccttggctc gggggctgaa ctggttaaaa ccctggttaa cggcggatat atatggctgc
     6481 tgtacggagc ggctatcacc tttgtccccg tagcgcttgc aaccatcgta ggaagatgca
     6541 tgaagatgaa ctacctccag ctttgcggcc tcgtttcagg ggcgatgaca gaccctccgg
     6601 cacttgaatt tgccaacagt cttgcaccgg tacatgcaca gtcaaccgcc tatgccaccg
     6661 tctaccctct gactatgttt ctgaggatca tcctggccca gacccttctc ttcctgaccc
     6721 tgtaaaggac agtgttcctt tttggtaaag ctggtactgg attatacctt tgtccggtct
     6781 taaaaactga acagatggta atgcgtacaa tcatggacaa tatcaggact tatgttctga
     6841 tggctttggg cctgctgata ttttcagtgg catggactgc cttcctgctg cctcaccaga
     6901 tatccggcag tggcgtctcg ggacttgccg ccatcatctt ctatgcaacc ggcattccga
     6961 tgggctacac ctacttcgcc ataaacgtga tcctgatact ggtaggactg aaggtgctgg
     7021 gtgcgggctt cggcatcaaa accatctatg gtatagtgct gggtgcggcg atgctgtcag
     7081 tgctgcagcc gttcattccc ggccccgttg tgcaggagaa gttcatgtca gccctgattg
     7141 gaggaggttt atgcggagtg gggctggcaa taatattcac ccaggggggc agtacgggcg
     7201 gaacggacat tattgccatg gttgtgacca aataccgcaa tataagcctg ggaagggtta
     7261 ttctttattg tgatgtagtc attataggtt cgtcatatct gatactgact gaaatggaac
     7321 cgatgcagag agtggagaca atggtatacg gttttgttac gatggcaata caggcttata
     7381 cccttgatac aattctttcg ggtaaccggc aatcagtaca ggtgtttatt ttctccaggc
     7441 attttgaaca gatagcagac cagataaccg gtaagcttca ccggggcgct accgttattg
     7501 acggcaccgg atggtacagc aaggagaacc agaaggtaat aatcactctt gtaaggaaga
     7561 atgaggcaag tgatctttac cgtatcatca ggagcataga cgacaaggcg tttattaccg
     7621 ttgccaacgt catgggcgtt tacggcaagg gattcgagga gctgaagagg tgacagccct
     7681 tccgtcgcaa aaaaaaagcc cgctcacgcg ggactttttt gttgctccgt cagggctcga
     7741 acctgaactt ttctgatcca gagtcagacg tgttgccaat tacaccacgg agcactgttt
     7801 tgaggctgca aatatataaa aatttcaact tatagtgtaa cgggatccgt ttttaaggca
     7861 atccggaatt aataaagtga catgctatgg gtctgtacgg agtattttgg ctggtaaatt
     7921 gtaactttga gtttcttggc actggaaata ataagcctat gaaaagaatg agaatgtttt
     7981 tagtggttgc ctgcacaatt gctgcattgc agccggcatc atcacagaag cttgaaaccg
     8041 acaatctcga aaaccttgtg tttcccaaat tccaggaatc ggttgtcaga ctgaagaacg
     8101 gacagtcata ccaggcggtg ctgaattatg agaagaccga aaggcaaatg gttgtgctga
     8161 gaaacaacca gttgtacctc tttaaggatc accaggctgt tgatacaatc tttatggccg
     8221 ggcggatctt cgtacctgcc gaaaccggat tttatgagtt gctggtcaat ggcccggtaa
     8281 cactttttgt caatcacagg gcaacccttg acaatacagg ggccacactg gcctacggat
     8341 caaaaaccac cacagccggc gtgacacatg tgaacaagat tttttcccag gatggcgcca
     8401 tcaatctcag aattccggaa aatttcaagg tggccgacga taccaacttt cttttaaggg
     8461 ttgacggtca gatccagaaa gtcctgaaca aaaaacagtt cctgaaactg ttcccggcaa
     8521 aaaccaggga actggaggag ttcataagca gcaacaatac cagtttcaaa tcacaaaatg
     8581 acctgatcag tctcgttgag ttctgcaacg cgctaaaccg ataggttatg tcaccggcag
     8641 caggcagacc tatttgttga tcttctgcca ggtatccttc agcccaacgg tgcggttaaa
     8701 gacgaggctg cccgggcgac tgtcataatc tgcacagaaa tacccgatcc gctggaactg
     8761 aaacttgtcc agtggcctta cgtcagccag cgaaggttca accttacatc cctttataat
     8821 tgtcagggag ccggggttga ggaactccct cgggtcaata tccttgtggc ctgacggatc
     8881 ctcgtcgctg aagagcctgt cgtaaagcct cacctcggca tcaagtgaat gtgcggctga
     8941 gacccagtgg agtgtgccct ttaccttgcg gtcagactgc ggacctccgc tgcgggtttc
     9001 ggggtcatag gtgcagtgga cttcattcac ctcactgtta ttatctttca ggagagcggt
     9061 gcattttatt atataggcac ctttcaggcg aacctcctgg cccggagcca gccggaagaa
     9121 tttgttggga ggattttcca tgaagtcgtc cttctctata aatatctccc tgctgaaagg
     9181 tactagccgt ttgccatgag cctcatcctc cgggttgttg tctatctcca tctgctcaac
     9241 ctgatcctcc gggtagttgg tgatgaccac cttcagcggc ctgagcacgc caaaaacgcg
     9301 gggagcacgc ttgttcagat cctccctgat agcgtattcc aggagagata catcattgat
     9361 agcttccacc ttggtgtaac caatcagatc aacgaatcgc ctgaccgact ccggtgtgta
     9421 gcctctgcgg cgcagaccgc acagcgttgg catacgcgga tcgtcccatc cgctgaccac
     9481 tccgtccttg accagctcga gcagtttgcg cttgctcatg acagtatagg tcaggttcag
     9541 gcggttgaat tcaatctggc ggggcctgta atcattcgtc ttcagctggt caataaacca
     9601 gtcgtaaagc ggcctgtgaa cctcgaattc aagggtacac agcgagtggg tgatcccttc
     9661 aaagtaatcg ctctgcccgt gggcgaaatc gtacatcggg tagactttcc atgttgtgcc
     9721 ggtccggtga tgaggggtct tgagtattcg gtagatgatg ggatcacgca tgtgcatgtt
     9781 gggcgaagcc atgtcgatcc tggctctcag cacccggctg ccctcctcaa attcaccgtc
     9841 gttcatgcgg tagaaaaggt cgaggttctc ctcaatgctg cggttgcggt acgggctttc
     9901 agcgccgggc tgggtagggg tacccttttg ctccgagatg gtctcggcgc tctggtcatc
     9961 cacatacgcg aggccgcgct ttaccagtga aactgcaaaa tcccacagcc tgccaaaata
    10021 gtctgaagca tagaattcgc ggtcttccca ggtgaatccc aaccacttaa tgtcttcctt
    10081 aatggaatca acatactcta catcctcttt ggtagggttg gtgtcatcaa accgcaggtt
    10141 gcactttccg ccatactttt cggccatgcc gaagtcaagg cagatcgcct ttgcatgccc
    10201 gatatggagg taaccgttcg gctcgggcgg gaaacgggta tggacccggc cgctgttctt
    10261 cccttcaccc aggtccctct cgatggcctg ctcgataaaa tgctgcgaat caggtttgat
    10321 tatctcttca gactctttca tttgctttgt tgatgaaccg tacaaaaata tacggaattg
    10381 gggcacgaaa ttcagacatg gcactttttt gatgaagctg ccggtcagca ggtgtcaccg
    10441 gagacctcag atagcgatgt tcataccatt tatgattgca gtctcatcgg acggctgcag
    10501 aagccgccat gactactacc tggaaacctc cacgctgacc cggcctatcc ttccgcccat
    10561 aacaggattg agcttggaaa ccctgacaat ggccttgcgg atacctggca gctcactgca
    10621 cagggcctca agaatacggg ttgcaatatg ctccaggaga ttggatgtct tctccatctc
    10681 cctcttgatg atcaggtaag ccgtctggta attgagcgta tcccgaagct catcactctt
    10741 cccggccttg tcagtatcgg tctcaatggt cacgtcaacc atgaaatgac tccccacaat
    10801 ctgttcctcg tggtaatgcc cgtggaaggc ataaaactcc atatcttcta tcctgatcac
    10861 tcccatgata attatattat tttatatact attgataaac agatcaatac gaactatttt
    10921 cccgttcagc caaaaaacta ttcagtttat ttggtttata acgtaaacta gtttatattt
    10981 gcatcgaaca atttggttaa aagaaaagta aaattaacaa aagccatgga aaatgaagaa
    11041 aaactgatga ccggggagga gagcctcagg gtcatctctg acatgatcag caagacgagg
    11101 gtgagtgtca cccaggcaag ctttcatctc cttttatggg gatggctgat ttttgcctgc
    11161 agcctttcag agttcctgct atggaagtat gccggctggg ctgactcgtg gtatgtatgg
    11221 tttgcagtca tagcaggggt ggttgcatct ttcgcctatg gttttgcgaa ggggaaacga
    11281 gagaggattt ttacgtacgg gacaaagatt tatgtatgga cctggattgc atttttctgg
    11341 gcctcggtaa tttttttcat agtttttccg cttgagactg gcgatgtggc aaaatacatg
    11401 ctgctgatgg cagggatccc gttgctgatt tcaggagtgg tgctgaattt ccggccgatg
    11461 atgctgggtg cagcagcctt ctggctgctt gcactggttg cgcacttcgg cggtgacact
    11521 gtatcgggac tggcaatgcc ggccgcgatg attatcggat atcttatccc gggttatctt
    11581 ctcagaaaaa ggggcagcca tgacaccttt cagggagctt gatccgctgc tgcattcgca
    11641 actgaggctg gcgataatgt caatactgat aagcgttgac agcgccgatt tcatctatct
    11701 gcgggagaag accgatgcaa cagcgggcaa tctcagcgta cagataggaa agctcaagga
    11761 ggcaggttac atctcggtga agaaaagttt cggagaaagt tacccgcaga cgatgtgccg
    11821 gatcactgac ctgggacgcc agaggtttgc cgaatatgtg gcagcgctga aggattatct
    11881 gagggtctga gggtctgagg gtctgaaggt ctgagggtct gttgcctact tccttttact
    11941 gaatcccagc ccggcttccg ggccgatggt ccagagggga gccttgtaaa ccgggcctcc
    12001 gctgacaggg cactcagatg cattaaatga cggatcgagg tcaatgaatt caaaaccccc
    12061 gacaccggct gcaaaatgaa ccgcacaccc cagcgccaga ttggtctcga ccatgcagcc
    12121 gatcatgagc ttgaggttgg cgctgcgcgc tatggcagct atatccagcg actcaataat
    12181 gcatgacttc atcagcttga tgttgatggc gtcggcacag cccgtacgca caatgttgat
    12241 ggcatcggca cgtgtgaaga ccgactcgtc ggcacccaca aggatctcgg tgttgtcacg
    12301 cacatacttc atcccggcca ggtcattctt gtgcaccggc tgctcaaaca tcaccggaaa
    12361 aatgccttga ttcaccacct cattgatgaa gcgaagcgcc gttttcggcg aataaccctg
    12421 gttggcatct atcagaatcc cgcattcagg ggcaccatcc ctgatggcca gcaccctctc
    12481 gacatcctcc ttcaggttga cgcccacctt tgtctttatc accctgaatc cctcggcagc
    12541 cagctgtatg gcctcctttt ttgcctgttc ggccggcaca atggctatgg tgtaatcagt
    12601 ctcaatcctg ttgtcagttc cgccaaggaa tctgtagaac gggatcttca gggtttttgt
    12661 gtaggcatca agcaaagcca tctcaatggc acaccttgcg acagactgtg cccagaacgc
    12721 actcttaagc gtgtatgcaa ttgacctgta gttgttgata tcctggccca cgatgaattc
    12781 cctgcagctg ttcagtgttc ccaggatagt ctgctggttc tcgccgctga ttgtcatgat
    12841 cggggccgct tccccgtagc cctctatgcc gttctcaagt acaatggtta caaaagcatt
    12901 gtcaagatgt gtcatcacgc caagggcgat aggaaaaggt tccatctcaa tgctcagtgg
    12961 ttcagcaaac atcttgctga tcttaaggtc tttcatccct tcctgtctcc ggaaactgcc
    13021 ttctccggcc ggaaacaccg atgcacccag tgcagtggcg gtggccagcc cgcctgcggt
    13081 cagaccgctt tgcctgataa atgatctcct tgtagtcatt gcctgattgc tttgatccac
    13141 taatctacaa aatcagatcg gatatcctgc aggaatcagg ctatgtttaa tgacacaaat
    13201 cctctagggc ctcatactgt ccattgcgcc aagcaggaat accagcgggg cgttccagtt
    13261 aatggctatc tcatttgtag agtagctgca ttccacgtcc gagtatgact ttgccgggaa
    13321 ggtagatctt tcaacaggcg gctggcagtc gttcatcaca acggtattcg gtccgcctac
    13381 aagaaatccc ggtacaggtt caggcactcc gtcagcgcct gaggggcggt gatggatgtg
    13441 caagggtgaa agggttccga acccggtcac aaagcaatac cccgtcgggt tggtccccag
    13501 cagccagttc aggtcggcct gcagggaggg cagataccgg tcactgccgg taattttcat
    13561 ggcaactatc ttgatcatgg cctggtttgc gacatcggaa gtgcttcccc atgcaaaccg
    13621 gtcaaggctt atgcggtaag ggcatttttc cgatttcccg gcaagctcgt cagcatatgc
    13681 ggtgagcagc gcggcagcag cttctctcag cgctgcgaag gaatcatcac tgctcagcgc
    13741 cagtgagatc agcccgagtg tctccgactg gttccaggtg ggtgtcaccg ccctgacgcc
    13801 atcaaggaat ccttctccgg gaacttccat ttgtgccagt gcggtttcgg cttttgccca
    13861 gaaaagttca tcggagaggt tgccatcacc gtatgcaccg gtgctgacat catcaggctg
    13921 acggtaaatc acatcaggat gagccaccgc ccacttcatc gctttatccg atgcctccag
    13981 gcatgtggcg gcaagggccc tgagctccac tgaagggtca tttgaaaaga cccggtaagc
    14041 cattgccgtc acggctgcaa agtcaagggt ggcggcagtg ctcttcataa caacatatct
    14101 tggttcagaa gcctcatgcg gcattacgaa tccgccaaaa gcgagattgg tcagcttatg
    14161 gtaaaccccg ccgtcagccg ggtcctgcat tgtgagatac catctcaggt tccagagcag
    14221 ttcgtcaacc agatctggta ttttatttcc gctctcagga atattaagtt ccagcgacct
    14281 gcagtattcc gggaacatct ggtaaaaaag aagcagggtg taattggtga tgctgctgtt
    14341 gacgatgtac ttgttgtagt cgcccgcgtc ataccatcct cctgggcttg agatgactgt
    14401 tccggccggc ctgccggccg aagcggctga ggagtgaatg atgaccgcag tatcaggatg
    14461 accggcagga cgcgcccatt tgcctccgaa ttcctccgtg atctcaatcc cggtacggtt
    14521 gaggtagtaa gagcgtaccg ctccgcgcgt gatctcactg tacacatctt cccctatccg
    14581 gaagggggcg gagcagatat cttttccctc caggcagatc ctgtatgtgc caggtgtggt
    14641 aaatgccgag aaatcggccg cacggacagt atcacccgaa agctcccagt agagaggttc
    14701 accgggctct gcgccataca catttctgcc tgtggagacg tcaatcagct caaagatgtc
    14761 agcgtcggct ctcagcagag ccacctttgt cgcatccgga agatacccgg cctggttcac
    14821 aaggatggca tcagtcgccg gaggagcctc aagtcttgcc tttataagtg cattgctccc
    14881 gcaacctgcc aggaaaaccg tgactgctgt tcccaggatt aaatttaatt tactgaatat
    14941 catggcatta catttttatt ttgacattat gttccgttcc ttcaggaaac acgggcagta
    15001 tgcatgaatc gacctttttc ccgtcaacgg taacctccca tactcccttt gatttccgtt
    15061 ccgggttgat tacctcgatg ttgtataccg cacccctgaa cctgcgcttt accgtaaagc
    15121 cgccccagtc ggcagggatg cagggatcaa ccatcagccc gtcatagtct ggccgtatcc
    15181 ccaggatgta ctgtgtcaca gcataatagc accatgcagc agtgcctgtc agccagctgt
    15241 ttttcgcctc accgggacgg aaggcgtcct tccctgctat catctgggag tatacgtaag
    15301 gttccgtttt gtgcaggtca cttatctcct ccaggtaggc tggtgttatc cggctgtagt
    15361 agtcaaatgc ccggtgaccg tttccggtaa tggtctctgc gatgatgatc cacggattat
    15421 tgtggcagaa aacgccggcg ttctccttgt atccctcggg gtagctggaa atctctccca
    15481 ggttgagaag gtactttgtg aaggcggggt tgttgagcac tattccgtac tcgcaggcca
    15541 ggtgtttgtt caccgaggcc agcgccttca gagccctgcc gtcatcaaga ccaatgccgg
    15601 ccatgatgca catcccctgt gattctataa agatttttcc ttcttcgttc tccctgcttc
    15661 caaccttcct tccgtaaaag tcatatgccc tgaggaacca ttcgccgtcc cagccatgag
    15721 catttatggc tttgaccatg ccttcaatct gcctggctgc atcatcagcc atctgcgctt
    15781 ctccccttcg acgggcgaag gtgacaaaat ctgccccgta gagcacgaac atggcggcga
    15841 tgaagagcga ctcagccact cctcccttct ggttttccgt tgtctggaag ctctcatcgg
    15901 gattcgacga gaaacaattc aggttgaggc agtcgttcca gtcggcccgc cctatcagcg
    15961 gcagtccgtg aggtcccagg tttttgacca cgtgcatgaa tgagcggcgc aggtgctcga
    16021 agaggcttgc cgcatcatca ggattattat tgaatggcac cgtctcgtca agtattgacc
    16081 agtctcccgt ctccttaagg tatgttgcca ccccgaaaat cagccatagc gggtcatcat
    16141 tgaagttgcc gccgatctcc gcgttgccgc gcctggtcag cggctggtac tggtgatatg
    16201 cgctgccgtc aggcaactgg gtggaggcga tgtcaataat cctttcgcgc gcctgtggcg
    16261 cgatcatctg aacatatccc gccaggtcct ggttggagtc gcggaatccc atcccgcggc
    16321 ctatgccgga ctcaaaatag gatgcactgc gcgaaaaatt ataggtagcc atgcactggt
    16381 acgggttcca ggtattgacc atccgctcaa gccgcggatc cgggtgggtg agggtgatca
    16441 ccgagagctg actcttccag tattccataa gtgccgcgaa ggcggcatca accttttcca
    16501 cggtgtcata ccgcccggtc attgccttcg cccttgcctt gtttatgacc ttcggggcga
    16561 tgaacttttc actgtctggg ttttcaacat aacccagcac aaagacatat tccttcacct
    16621 cacccggctc aaggtctgca acgaggcagt gtgaggcaac aggcgaccag ccatgggcaa
    16681 tactgttccg cggttgtccg gcgaagaccg tgtcaggcct gtcaaagccg ttgtaaaggc
    16741 ccagaaaagt ttcacggtca gtatcaaaac cggcaacctg ccggttgacc gaatagaaag
    16801 cataatggtt tcgccgctcg cggtactccg tcttgtgata gatgaccccg tcttcaacct
    16861 ccacttctcc ggtggagaag ttgcgctgga agtttgtctg gtcgtcatgt gcgttccaga
    16921 ggcaccactc cacgaacgag aacaggcgga tgcgcttcgg catcctgccg ttgttgcgga
    16981 gggtgattcg ctgcacctca caggtgtcgc ccagcggaac gaagaagagc atgtcggccc
    17041 gcaggccgtt cttttccgaa ccaatccggg tgtaacccat gccatgatga caggagtagt
    17101 tttccacatc ggccttcacc ggtttccagc ccggagacca gacagttccg ttatcattga
    17161 tatagaaata cttgcctccg tcatccgccg gcacattgtt gtacctgtag cgggtgatac
    17221 ggcgaagacg tgcatcacgg taaaagctgt aacccccggc agtgtttgag attatggaga
    17281 agaagtcctt gttgccaagg tagttgatcc agggccaggg tgtacggtaa ctggtgatga
    17341 catactcctt cgcagcgtca tcaaaatgtc cgaatttcat tgtcaggtca gtcttagagt
    17401 ttttttcatt ttcaattccg gcattgcctt gagaccggat acaggtgtca ccaccgggtc
    17461 agtccttaag tttatcccac caggtgaatt tcaggatcac ggtggtaacg atcatcacag
    17521 ccacggcaac gtagaactgt ttccattccc ttatcacaag aaaaacgggg aatgccatta
    17581 gtgagagctg ccatacgata cctacggcaa tattgaacat atcgcgccag aaatccctgt
    17641 tcggtttgaa ggagggatcc tcggcaagga ccattttctc taccggtttc cagaatcccc
    17701 acgggcgtac ctgcctgtag aacctcttca gtgtctcatc gtcttccttt ttggtgagaa
    17761 gtgatcccag gatgcttccg accagtgaga taaggaatat taccgggaag gcattcatgc
    17821 tgaagttctc agtcagcggc cagttctgaa gcaggtcaag gttcagcgca ggcagcagga
    17881 gggcagcggc aatgcctgag agcatacccc agaagtaacc gtagccattg aaccgccacc
    17941 agtaccattt caggaagttg ggggcggtat aaccgcccca cagcgctgct gtgatccaga
    18001 gcacgacatc attgatggat gtgaccagga agcccacggc tattccgatc accactatca
    18061 gcagtgaggc agcgtagctc atgcgcacat aggtcttctc gctggcatga ggattgacgt
    18121 accgcttgta gatatcattc accaggtatg ccggggcggc gttaaccgtg gctgcaaagt
    18181 tgctcatgaa ggcggctatc aggcctgcaa gcaggaaccc gagaagacca accggtacgt
    18241 atgttgccag cacctcaggg aggatgctct caaagtcagg gttggtgagt gaccctgtat
    18301 agagcttgtc gaagttcacc agggcaagga tggtgaggcc tgccaccatg aagtatcggg
    18361 tgggattgag gaccacactt acaagtgagc tcatcttcga cgcttccttc gggtttttgg
    18421 ttgcaaggat ccgctgcatg tcatagtttg gcgccgggcc tgctgcagag acaagtatcc
    18481 ccttgaagag catcatgaca aagaagagcc cgaagagctc gtatccgtca gtctttatcc
    18541 agtcattgaa tgagtggagc ttcggcgaag ccgctgagga aatggtatgc cagtcaagtc
    18601 ctgttgtccg tccgaaccac attgtcttcc atccttcggg aataaccgaa ttgagtgcat
    18661 ccggagagat ctggctgatg gcaattattc ccaccgctat ggatgctatg gtcagtattc
    18721 cgtactgtat gacttcggtg atcaccacgc tgaacatacc acccttgacc acgtagaatg
    18781 tggtaatggc catgaggatg agggcgtaga ggttgacatt cacctgcggg tgagtgacca
    18841 ggtactcagg gtttgaaacc agcggcggga ggaatgctgc cacgaatttg cctaccccct
    18901 tgaatccata ggagaggaat ccgataacgc tgacaatggc aaatatcacc actataatgt
    18961 gtgacaggtt tgcagccctg ccttcaccaa agcgcgtctt gatccactcg gctccggtca
    19021 ttacgttcga tcgtctgagc caggctgaga ggaacaccat aaggacgatc tggttgaaga
    19081 ccggccatag ccagggtatc cagaggctct tgaggccata gaccgagagc cagtagacaa
    19141 gaagcattgt tccggctatg tcaaacatgc ctgaagcgtt ggatacaccc aggaaatacc
    19201 atggaaggga gttgccgccc agaaagtagg actgtatgtt tcttgatgcc ctctttgaga
    19261 cccagttccc gatgaagatg gtggccagga ggtacaggat cactataccc aggtcaatga
    19321 tatgcaagtt catagtggtg gtggttagtt gggtttattg tcagataatg aatgaaatgc
    19381 gtcccttgct ggttttctcg tacacctccc tgctgaagag gtaaaactga gccggtttgt
    19441 gcggcactcc ctgctgtttc ttctttaacg ggatgacgta gtgcatctgc gccacctttt
    19501 tcctgaagtt tctcttgtca aatgatgttc ccagcagtgc ctcgtacagt ttctgaagct
    19561 gtgacagcgt gaagtaatca ggcagcagct cgaaggcgat aggctcaacc ttcaccttgc
    19621 gctgaagggc ctcaagcgcc ttataaaaaa ggtatttatg gtcaaaagcc atctccattt
    19681 ctgctaacat ggagacagga aaccaggaga catcacggtc tttcagggta agtgtgacct
    19741 tggtgttgtc tatcagcgcc aggtaaccta ccgtgacaat gcggtcagca aatccctggt
    19801 tgatatattc aagccacatc ttgtccttgg gcgaggagac gcggtcaagc tggccgaagg
    19861 caccgaactg ctcgaggaag atattgtcaa gccctgtcag ccctttcagt atcctggtgg
    19921 ctgcactgtc aaggtcctca tcctcgaaaa tatggtaacc ggcaagggta tggtcgagga
    19981 tgagcacttt tccgctcttc ttgtccacca tctctctttt gacaagcagg acatttagtt
    20041 tttcaaagtc gaaaccgaac accacacagt cgacagagat gttgggaaca atcttgtggg
    20101 atatcatttt attataccag tgaagttata aaatgactta gtgtacaatg taagaattca
    20161 aagatatttt aaaaatatag cataatcaac accatcagga aaaaagagct gatgaaaata
    20221 ccgggagagg tacgggtgaa gtatctatta atatggtata agatgagctt ccgcaggact
    20281 tgaagtaagt agtaaaacct gttgccccct gacatctgcg taattatttc atagctttgt
    20341 gtatattgta caccaagaaa tgccggtttt gtcttttaag gtgcagtact aatcaaatat
    20401 ttacaataaa gagacaaaaa agtatcagtg ctgcgctgac aagataccgt aactttagaa
    20461 acctaaaact gtacagctat gcaaaacaaa ctcttaagac taacatttct tatttgtctg
    20521 gcagctgtaa tgtcgacaac gatgcttttc gcgcagcaga gaactgtgac gggaagggta
    20581 gttgatgaca acaatgctgc gctgcccgga gcctcagtag tggttaaggg cacgacgata
    20641 gggactgcta ctgatctcaa cgggaatttc accctgccgg taccttctga tgccacaacc
    20701 cttgttatca cattcgtcgg aatggatgca aaggaagtgc ccattactgc aggtcccata
    20761 aacgtcacac tgacgatgtc tgctgaggaa gttgacgctg ttgttgtgat tggttacggt
    20821 acgatcagga gatcggaagt gacatctgca attgcctcgg tcaagactga ggagatccag
    20881 gaccttgttg taaccggcgt tgaccaggcc ctgcagggca aggtggccgg ccttcaggtc
    20941 atgaacaaca gcggtcagcc gggcggcggc gtctcggtaa gggtaagggg tatcacctcc
    21001 atcaacagca atgacccgct gatagtaatt gatggtgttc cctttaccaa caacaatgtt
    21061 gaaaatactg gatatgacgg cctgggaggt tctgacggcc agacccagaa cagcttcctt
    21121 gcaactctga accccaatga tatcgaatca atcgatgtgc tgaaggatgc ttcggcacag
    21181 gctatctacg ggtcgcaggc agccaatggc gtcatcctga taaccaccaa gaaggggaaa
    21241 gccggtgacg ggaaagtcac ctatgatttt tcttatggcc tccagcaggt gggcaggacg
    21301 cttgacgtaa tgaacctccg tgaatttgcc gagtaccaga acagcgtaat cgctgaactt
    21361 ggttccggat acatacccac cgaggagttt gcggatccct ccctgcttgg tgaagggact
    21421 gactggcagg atgcaatatt ccgccaggga tacaccatgg atcaccagct gggcatctca
    21481 ggagggaagg aaaatacaag ctactacttc tctgccggtt actttgacca gaaaggaatc
    21541 ctgattggct cagacttcaa gcgctactca acccggttca accttgacag ccaggtcaag
    21601 aaatggctga gggcagggat cagctcgaac atcaccaggg ctatccagaa cgttaccctc
    21661 gcagatgctg cagagagtac catatggtgg gccacacttc agaaccccct tatcccggtc
    21721 aggaaccttg acggcacatg ggccggcaac tataccgtgg gaggctatac ctacactgct
    21781 gacaaccccg tggcaaccag caacagccgt ggcaacaagg gtgtcaacag ccagatattc
    21841 ggaaacattt atgctgatgt gatattcttc gagggattgt ctctcaggaa tgagttttca
    21901 taccagctcg ggcttcagaa caacatggca ttccagtatg aagcaaatat aggaaccagg
    21961 tcgctgcagg cacagctcta tgacagccgc aacaactcct attattatgc tgtgaggaac
    22021 tacctgaact ataacaagaa cctcgggaag cacaaactgg ccttcaccgg cggtcatgaa
    22081 gcacagtact cctactggga gaacatggga ggaaagaagg ttgacctgca gaacaatatc
    22141 ctcgatatga acgcaggcgg taccgacaag ctgacatggg acctcaccgg cggcaagggt
    22201 gactgggcaa tggagtcata tttcgccagg ggcaattata ccttcgatga tcgttactca
    22261 ctttcgctca gttacagggg tgacaagtca tccaacttcg gcccgaacaa aaagtggggc
    22321 ttcttccccg gtgcatctgt aggatggact gtcacgaacg agaagttcgc cgctaattgg
    22381 gccaatgtaa tgaactacat gaagataaga ctcggcgccg gtgccgtggg taaccagaac
    22441 ctgcccggcg gagctcctaa cccgccttac acgtcaaacg tcaacttctg gcccggcccg
    22501 acaggctttg gccaggtggg gacggcatcg accaacttca tagccggtat cgccaaccct
    22561 gacctcggat gggaatcagt aatcacatac aacggaggga ttgacttcgg ctttctgaac
    22621 ggaaaaattg acctgaccgt tgacctgtac aaaaaggtca catccaacat gcttctcttc
    22681 agcacgggtc ctgccctgct gggtatcggt gatgcatgga acgacctcaa ggcgcccata
    22741 ggaaacgtcg gtcagatgac caacaccggc attgacatca gcctggtgac gcgtaacatc
    22801 tcgaagccca atttctcatg gaacacctcc tttatattct cgcactacaa gaacgttctt
    22861 gacaaactga tcaatgagtc atcatcaata gacggcaagc tctattatga caactacctg
    22921 atcacccata ccgttcccgg ctatgcggtc ggaaccttct gggggctggt gactgacggg
    22981 ctcttccgca cagaaactga ccttgccaac tccttcccgc agttcgggtc agccgtttct
    23041 cagaatgaga catggctggg tgatgtgaga tacaaggatg ttcataagga ccaggtgata
    23101 gattcaaagg accttacttt catcgggagt ccgatacctg acttcacctt cggtctgacc
    23161 aataccttcc gctacaggga tcttgatctc tccatcttcc ttcagggaag ctacggagcc
    23221 gagatattca acttcatgaa atggcagctc gagaggatga acaacgtcta ttacaaccag
    23281 tcgactgctg tcatggaccg ctacactgcc accaacaagg acggagcgct tcccaggttc
    23341 accaacacca acaccaacaa tacggccatg tctgaccgtt atgttgaaga cggctcctac
    23401 ctgcgtatcc agaacatcac tctcggatac aggctgccag gaactgtgat caacagggcg
    23461 cagatgagca acctgagact gttcgttacg gtccagaacc tctacacctt caccaattat
    23521 accggctatg atcctgaagt tggctcttac aacaacagca tcaggctgat gaacgttgat
    23581 gcaggccact accccaatcc gcgcacatac atgggcggtg taagtgttga gttttaatga
    23641 ctataactaa atgatcatga aaaacagatt tatatatttc attgtgatac tggctgcaac
    23701 acttgcatca tgcagtgagt cgttcactga ccgcccgtcg ctttcagggg caaccgtgag
    23761 caactactac aatactgccg atgaggtgag ggctgccacg agcacaatct attcgggact
    23821 ggcatggtcc ggatatgaag cccgtgccct cgactgcatc ggtgaaatcc ttgcaggaaa
    23881 cgagcatacc tataacggag atgacacccc gtttcagctg atgaccgtct ctgccacctc
    23941 ggtgcgtctt tctgactcat ggaaagcctt ttacaaggtc aacggatgga caagcgcact
    24001 gattgccact ttcgagcaga agatagccga cggcggtgac gcggcaatac ttgaaccggc
    24061 aattgccgag tgccatttcc tcaggggaac ggtctatttt tacatcgccc gcatatgggg
    24121 acatgctccc atcgtaaccg acccgggcgc cattgccctg acaggtgatt tcaacattcc
    24181 ccgctatttc caggaagatg ttctgcgctt cgctctcgag gaactgcagc ttgctgaggc
    24241 aggtctgccc gaaacagatg tccccggcag ggtgacaaag tattcggcca aggggatgat
    24301 ggcaaaactc tatctctatc aaaaggatta tgccaatgcc aaggcaaaag cctgggaggt
    24361 aatggagtca gggcagtatg acctcttccc cgattaccgc gccatgttca actcaagcga
    24421 caacaacaac aacatcgaaa ccctctttgc agttcagcac cagtgcacga tgaacccctg
    24481 gggatcaggc aaccagctca acccagaccg cggcccctca agccttcaga ctgatgaagc
    24541 aagtatgtgg gagctttaca gaccatccat ggacctcagg acggcctatg aacccaatga
    24601 caagagacgt gcaggctcga tgatggagca tggctggaac atggctgact ggaagccgca
    24661 gaagcttccg caggctgacg gatcattctc acctgacgat gcagcttaca atgatttcat
    24721 gaaggacggc ttcaggtatg ataccatcca ggatgttaaa cagggaggca cactgaacgg
    24781 aacacgttca accattgcaa aatatgttgt cggtccgggc cagaagtatg gcggtgagca
    24841 ggtcataggc atgaacaccg gcatcaattt catgatgcag cgctatgccg atatccttct
    24901 tatctatgct gaagctaccc tgggtgaagc tgcctcaacc actgacgcaa ctgcccttga
    24961 ggctttcaac aaggtgcgcc ttcgtgccgg tttaccggta aaagaggttc tgacacttaa
    25021 tgatatcatg aaggaacgtc gcgtggagtt cgcctttgaa ggtgattact ggtttgacat
    25081 aacccgtcag ggttttgaaa aggccaaaca gatcattgaa gcacaaaaca ggggaactat
    25141 ttcgggtatt gtccgtgtca caggatttac cgaagacaag atgtttctcc cgatccccgc
    25201 atcggaggtg cttcaggatc ctttgcttaa cgaagatccg caaccctatt acaccaaata
    25261 gagatacctg ttatgaaaaa gataataaac aatacattat cggcattatt gctgctgctc
    25321 cttgcgggag ccgcaggaat ggtgttccag gcatgcgagg gcaatgagga agatatgccg
    25381 atgcctgaga tcttctacat ccggaaggca gacccggcca agtcagactc actggtggcc
    25441 catgcgttca tgggtgacaa catagccata ataggcagga accttggcaa tgttgacgag
    25501 gtctggttca acgaccagaa ggcagggctg aaccccaacc tggtcacacc taccacgatc
    25561 atcgtttcag taccgcaggt aatcccgtca acggttacca actacatgta cctgatcaac
    25621 atgaacaaga aggatacgct gaaatatact ttcggcgttg acgttcccgc tccgctggtt
    25681 gacaggatgg tttgcgagta cgtggctgac ggcgaaacag cagtgatcaa aggcaactac
    25741 ttcgtagatg accctgcttc accgcttacg gtgctcttcc ccggaaatgt ccccggaacc
    25801 atcgtgagtt tcacaatcaa cgagattcac gtgactgttc cgtcaggtgc aggtcccggc
    25861 cagatacagg tcaagtcgct ctacggtacc acccgttcac gcttcttctt ccgtgatgac
    25921 aggaacatca tcctgaattt tgatgacctg acagctgccg gcggatggcg ttcgggagtg
    25981 atgggtaact cgaatcctga aggtatatca ggtaattatg tcaggttctc gggtgctctt
    26041 gccggaggag ccggtgtaac atggaacgag gacggattca gcttcaacct ctggccgcag
    26101 gcaaacggca ggcctgatgt gcctgtttat accggcgaac tgaaggatgc tgagatcaag
    26161 ttcgagataa acgttgttga agcatgggaa tcatgcgctc tgcagatgat cttcactccg
    26221 tactccgtca cgggagcaaa cagctacatt gcggacggct ctgtaccacg gggtgtctgg
    26281 aatccatgga aggcgacggg cacttacaag actgccggct gggaaacagt atcatacccg
    26341 ctctcagagt tcaactacag cttcgacggc tcggtcagcg ccaataagct caccgctgac
    26401 atgatgcgcg ggctgacatt ctatgtctgg cacggcggcg tggcaggcaa agactgcaat
    26461 ccgcacatct gcatagacaa catacgcgtt gtaccgaagt aacatcaacc gaaaaaagaa
    26521 ttgcaatgca taagattcaa ataaaattcc gcaactgggc gcttctcttc gcgacaatat
    26581 gcgtggtggg tgccggtctt ctgttcacct catgcgaggg gaaggaagag gaagatacca
    26641 aggtggttct ccagagcttc gggccaatgc ccatagccag gggagctgaa ctgaaattca
    26701 tcgggctgaa ccttgataag gtgacggcag tgattctgcc tgacaatatc accatttcta
    26761 cattcacaaa aaaggaagcc cgactgctga cactcaccgt accccaggaa gccgtgcccg
    26821 gttatgtggt gctgaaaacg cctgacggtg atatcaccac aaagaccaca atcggtttct
    26881 cggagcctat ctccatcgct ggtttgactc ctgcaacagt caaggcggga gacgaactga
    26941 caatcacagg cgattacctg aacctcgtgg aggaggtgat tctcactgac cgtgttacgg
    27001 ttaccgaaga tgacttcacc agtcatacac gcaccgagat aaagctggtt gtgcctgcag
    27061 aagcccagac aggaaagatt gcagtatcaa acggtgctga ggagccaata attgtgtact
    27121 cagcatcaac cctcacagta accctgcctg catttacaac cgtgactccg aatcccgtca
    27181 aggccggcac caatctcacg attgccggta ccaaccttga cctggtgctc tcagtcaagc
    27241 tcggcggcgg aaaagtgatt gaagcagcga atttcgtctc gcgcaccgcc acacagattg
    27301 ttctggctgt accggctgac acgaaggacg gcaaggttat cctggtgccg gcttcaggtg
    27361 tcgaggtgag cacagcaaca gacctggtaa tggttgtccc gaccgtctcc gtcaccccgg
    27421 tgacgcttaa aaacggtcag gatattacag tcaccggtac caatctcgac ctggttgacc
    27481 atgtcatttt cggcggcgac aaacagggaa caataaaggc aggcggcaca gccacacaga
    27541 ttcttgtgac agttcccgat gacgctgtcg acggtgttgt gaccttcgtc acgaaggctg
    27601 acaaggagat tgccggaccc aatcttacga tgatcgttcc cgcattctca tcgttcagtc
    27661 ccacctcggc aagggccaac tccaccgtca cactttcagg tactgatctt gacctggtga
    27721 ccaaggtaat attcacaggt ggcctggaag gctcaatcgg gacaagaaca ggaacatccc
    27781 tggcagttac agtcccagtt ggtgccaaga caggcaagat caccatcgtg accaaaaacg
    27841 gcactcaggt tgtatctgcc attgacttta cccttcttgc caatcttccg acgttcggtt
    27901 catacaccga aacacgcggc gaacccggta aaatactgac aatcaatggc accaacatgc
    27961 tccttgtcaa ggaactcata ttcccgggcg aaaagaaagc aactgcttac ggatcgaaga
    28021 gcgatactcg gatcgaagta tatgtacctg aagatgtcac ctttggcacc ggcacaataa
    28081 ggcttgtgac ttatgaggga gaagagggat tcttccctga actttacttc ggaggaactg
    28141 atccggtact taaccagaca ttctgcttct ttgacttcaa cggtacgggt aaggactcat
    28201 ggtggggcaa tgccatcgga agcggaatcc ttaatgatcc ggccagctcg gcagacggaa
    28261 ctccatactg gaatatcaac ggtatgggtg gctccggatg gtgggacggt ctcttcttcc
    28321 gcaacggcag caataacttc gtgacaaccg gcgtcgatgt caacacctgg gcagtcaggt
    28381 ttgacataaa cgttcgcgaa gccataaccg aaggcgacct ccgcataagg ctgggaagct
    28441 acttctatca cttccagcca tggaacggcg ttgccggagg atttaagacc accggatgga
    28501 ttactgttac atgtccgctg acaggattca agaccgatga tggcacagca tctattcctg
    28561 atgccgctct cggcggaagt gaattcggaa tgatatggag tcatggtgtt tccgtaaaag
    28621 ttaacatggg aattgataat gttcgctttg aaccgataaa ctaatttgat attcccttat
    28681 acctgataca aagggtatct cagattttat cactaaattg agataccctt tgtttttcaa
    28741 aaacaaagag agcaatttta ccggatgaga ctttttacca ggttagtgtt gcctgttgtt
    28801 ttgcttttgt caggcgccgg accagctcct gcacagggaa cgaaatatga agccgagaac
    28861 ggcaccctga ccggatctgt ttccgtgcag acaaccgttg caggattttc gggtaccggc
    28921 tatgcgggga tattcgaaaa tgacggtgac aaagcggcag ttacattcaa cctgtctgca
    28981 gccggcaatt acgatcttta tataggttac gccggacctt acggcgataa aaagaatatc
    29041 ctcacaatca acagcaacag tattgaggcc tcctttccgg cttcccctgc cttcactgag
    29101 acagcctgcg gaagagttag gcttaaggag ggcagcaata ccatttcaat tacaaagagc
    29161 tggggctggt tcctgcttga ctatttccgc attgaacccg atacgaagcc cgatgtcagt
    29221 gttctgctgc cgtataagct ctcaacccag gcgcctcacc gtgagacccg aatgctctgg
    29281 agttacctga tggacagctt cacgcacagg attcactcag gcgccatgag cctcaacgca
    29341 aaggaagagg ctgaatggct gtttgcccag acaggaaggt acccggcgct gacaggactg
    29401 gactttatga accatacccg caactacagc tggtatgaca aatcggtagt ggtgaatgag
    29461 gccaggaact ggtacaaccg caatggcctt gtggctatat gctggcactg gcgtgacccc
    29521 tcaagggcca ctgaggagtt ttatacgtcg ggcacatcgt ttgacatctc aaagatcaca
    29581 gaaaccacct ctgcggaata ccaggcgatg ctgagtgaca tcgacatcat agccggttac
    29641 ctgaagcaac ttaacgccga taaaattcct gtccttttca gaccgcttca cgaggcctcc
    29701 ggcaggtggt tctggtgggg cgcaaaaggt ccggaaccat gcaaggcgct ctggcggctc
    29761 atgtttgacc gcctggtgaa ttatcatggc ctgaaaaacc ttatctgggt atggacaacc
    29821 gatgccgctc ctgacaacct tgaatggtat cccggtgatg aatatgtaga tatcctgggc
    29881 gctgacatct atgcagctga cggtgacttc agctcacaac tgcttacata tgatgcaata
    29941 aaggagaagt ttggcggcag aaaacttgtc accctgagcg agaacggccc cgttccggat
    30001 cctgaaaggc ttatggctga cggggcccac tggtcgtggt tcatgccctg gtacggctct
    30061 ttcatacgtg acggcatcaa gaatccgctc agccactggc aaaaggtcat gtctcatgac
    30121 tatgtggtca ccctcgacga gatgcctgac ctgaagaact acccgctgag tgacgagcct
    30181 gactacagta atttcccgca gggtttcttc atgaacggat ggaaaccgag gacagctgta
    30241 gtacctgact ataccgatgt gcctgcagtc accgatccgg ttaccgtagc cgttactgtt
    30301 gactgctctg acacggttac gctggtatca ccgtatctct tcggtgacaa tgccaatctc
    30361 tggaccgggc cgatgtctga caatgccacc ctgatgaaga acatcaccaa ccgcgaccag
    30421 ggagttatgc gcgggccggg aggaagcacc tccgatgcct tcttctggaa caggaataca
    30481 aggccgccgg atgtgccgga aactcttctc aacgatccca ccaacaaaag ctggccctgg
    30541 tacggccaga gggcggagaa ctggactatg catgttgatt cattctacag catactgagc
    30601 aaggcgaaca ttaccggaat gctcaccgtc aactatggtt atgcaaggta cgggacaagc
    30661 gccgatccgg tagcacaggc cgcccatatg gcagctgact gggtccggta tgacaggggc
    30721 aggacaaagt tctgggagat agggaacgaa gtgtatggca actgggaggc cggttaccgg
    30781 attgaccgtt cactcaacaa ggacgggcag cctgaataca taacccctca gctatacggg
    30841 cagcactgca gggttttcat tgactccatg agggctgcgg ccgcagagac aggcttcgat
    30901 ataaagattg gagttgttat ggttgaagcc gcctcaacac attcatcgtg gaacaccggc
    30961 gtagcctccc gggttggtga cctggcagat ttttatattg tccacagcta ttatacacca
    31021 tggaacacag actctgatgt ggcaacagtc ctgaattcct acaaaaacac ggaaggatac
    31081 aaaaactatg tctggaacgc cgttgctgcc gccggaatgc cgcagttgcc ggtggctctg
    31141 acggaataca acatctttgc ggtcggctca aaccagcctg tctcccacgc caacggcatg
    31201 caggcagtcc tcgtcactgg cgagatgatc aggacaggct acggtgccgc ctgccgctgg
    31261 gaccttgcca acggctggga taatggcaat gaccacggaa tgtactcata caatgagccg
    31321 tttataccta attacacacc tcacccggca ttttatcatc tttattacat gcgccggcat
    31381 accggtgatg tgctgctgag atcaacagtc accggcgccc agggagtggt catcacacct
    31441 acggcattca gctccggcca cctgggcgct tcaattgtca atacaaccaa ggttcacaag
    31501 gtggttcgcc ttaacctgaa ggattacggg gttggtgacc gcttctacac ctatactctt
    31561 acagggacag aggggcagga tttctcaagg aaggtgttcg tcaacggcac cggcggcaca
    31621 ctgacggccg gaggtcctga cagctatgaa acaatcaggg cggatgcggt tgttatcggc
    31681 gatgagataa ggataaatct tcctcccctt tcggccgtct atattctggt tgagcccggc
    31741 acaaggcagc tggccatcag taacgaggtg acttccgctg acccggtaag gtctgacgat
    31801 tctgtcacaa tctggcctaa tccctccgaa ggtaacttca ccgtcaccgg tatgcctgat
    31861 catgtcagca gaatggagat cagcgatctt cgcggaaacc ttctgctgag cagggaaaca
    31921 ggtcgtggag aacatgaggt tacccttgac acagactttg tttccggtat ttacctggta
    31981 actttattcg gagataacta cactgtaaca aggaaattga taatcaaata ataacccgat
    32041 atgaaaatga gacgatcact gactgtactg tttacagtaa cggcactcat cgcggccctg
    32101 gcgaaccagt cgtgctccag gacaccatac gaacggactg ataacggggt catcattcgg
    32161 ctgaacggta agtctgacca tcccggtcag gctgtgaggc ttgttgtgat aaatgaaagg
    32221 ataatacggg tttctgcagt tccttccggt gagtttccgg aaacagccag cctcatggct
    32281 gtggcgcagc ctgatgacaa tatggatttt accatcgaga agggtgacgg gctggtaacc
    32341 atcagcactt catcgctcag cgccgaggtg tcactgaaga cgggaggagt ggtatttacc
    32401 gataatgacg gaactgtctt tctctctgaa ctggatggca gtgggaggag cttcatgcct
    32461 gtcatggcaa ttggtgaaaa aggttacacc gtcaggcagc agtttgagtc ttatcccgat
    32521 gaagccatct acggcctcgg agccaaccag acctctttca tgaacctgaa ggggaaagac
    32581 gctgatctct ttcaatataa cactctggca gtggtaccat tcattatctc taaccgcaac
    32641 tacgggatcc tctgggacaa taactcgagg acaaaatatg gcgacatccg cgactggaac
    32701 gaactctcct ccctgaaact ttatgacagc cagggtgccg agaacggtct gacagcgacg
    32761 tatgctgacc ggaagacagg aaagaaggta ttcaccaccc ggaacgaaag ggagataagc
    32821 taccagttca tacccgacct tgcagaattt cctgaagggt ttaaccttgg agacggaaag
    32881 gtaatctggg agggggcgtt tgaggctgac acaacaggga ttttcaagtt catgttcaac
    32941 tcggcaggtt atgccagact ctggattgac ggcaggctgc tttttgaccg ctggcgccag
    33001 tgctggaacg cctcaaccaa ctatttcacc ctgaacgttg aacagggccg gcgttatccc
    33061 ctgaagatag agtggatacc tgacgggggc gagtcataca tagccctcag gtatctcacc
    33121 ccccaggatg gtgatgaaca gcagcgcatc tcgttctggt cggaggtggc tgaccagatc
    33181 gactactatt tcatcagcgg cgaaaacatg gatgaggtta tcaggggtta tcgcaccgta
    33241 accgggaaag cccctgtaat gcctaaatgg gccatgggat tctggcagag ccgccagcga
    33301 tacacaaacc aggaggagtt gcttggtgtt gtcagggagt acaggaagag aaacataccg
    33361 tttgacaaca tcgttctcga ctggcaatac tggccgatag acaaatgggg tgatcaccag
    33421 tttgatgcaa cccggttccc tgatcctgac ggcatgatcc ggacactgca tgatgacctg
    33481 aacgcaagaa tcatgatctc tgtctggccg aaatactacg tcggcaccgg aaactatgat
    33541 gccatgaaga gcaagggtta tctctacatg cgcaacgttg agaaggaacg caaggactgg
    33601 atcggatacc tctccacatt ttacgatgca ttcaatgctg atgcaagggc tgcgttctgg
    33661 gatcagatca acaacgcact ctacagcaag ggtatagacg cctggtggct tgacgccacg
    33721 gagccggata taacatccaa cctccccatg gatgagcgca aggcaatgat gagtccgaca
    33781 gcactcggat cggcagacag gtactttaat gcctattcgc tcgtgcaggc tcagggtgtc
    33841 tttgagggac agcgggccac tgacccggag aacagggttt tcatcctcac ccgttcagcc
    33901 ttcgcaggtc tgcagcgcta ctccgctgcc aactggagcg gtgacatagc tgcaaggtgg
    33961 catgacatgg cagcacagat accctgcgga ctgaacttct cgatgtcagg gatcccctgg
    34021 tggaccatgg acataggagg cttctccgtg gagagccgct atcataatcc gtccccggct
    34081 gaccttgacg agtggcgcga gctgatgaca cggtggcacc agtacggggc atttgtaccc
    34141 ctcttccgtt cacacggcga gttccccttc agggaaatgt acaacacagc tcccgaaaac
    34201 catatagcct ataagacaat ggttgaatac aaccaactga gatacaggtt tatgccttat
    34261 atttactcgc tcgccggaca ggcatggctc aatgattaca cgattatgag agggctgacg
    34321 atggatttca gccttgacac tgcggtgttt gacattgccg accagtatat gttcggccct
    34381 tcgctcatgg tcaatcccgt gacgaaatat aaagcaaggt cgcgtaaggt atatctgcca
    34441 tcggcgtatg gctggtatga cgtgcgcacc ggaaggcatt acgccggagg gacagttgcg
    34501 gaagcagatg ctccctatga atggatgccg ctgttcgcca gggaaggatc ggtcattccg
    34561 accgggcctg aaattcagta tgctgaggag aaggaggctg atccactcac attatgggtc
    34621 tatgccggcg ctgacggcgc ctttgtgctg tatgaagatg agggcgacaa ttataattat
    34681 gaggatggag cctataccct catccccttc acctggagcg aggctgaacg taccctgacc
    34741 atcggaagaa gggaggggga ataccaggga atgctccatg agcgaaccat aaatgtcata
    34801 atggtatctg agtataaggc tgtgaagctt gacttcggac gaacccccga caaatcggtt
    34861 acgtattcag gggaggaaat aaaggttaag tttgagtaat ttctgatgtt gaggtataac
    34921 tgagataaac agtttaaatg attgtgggaa tgaagaaagt aatcactgtc atgacggcaa
    34981 tgctgctgtt tgcagcaact gtcgcggcac agagcatcgt gcctgctgag tggaaattcc
    35041 ttacgggaga caagcctgag tatattgact cggcaaagaa cgaatactgg tggaaggata
    35101 tctccccgct taccgtatgg gagaggcagg gcttcccggg ctatgacggg ttcgcctggt
    35161 acagggtgaa ggtggttgtc ccggcttcga tgaaaaagaa tgcagagaag tatgacgggc
    35221 tgatgcttaa cctggggaag attgatgatg ctgacgagac ctttttcaat ggccataagg
    35281 taggggcaac aggcaagatg ccccctgact atgccggagc ctatgatgtg ccccgcacat
    35341 acctgattcc tcctgattac gtgaactggg gcggaaagaa taccattgct gtccgcgttt
    35401 atgacgccgg gggcgacgga ggcctgtatg ggggtccggc cgagctgacc aacaggggca
    35461 atcctgacct gctcaccctt ggtactctgt tcgcccagga ggatatgatc ctgaaaggat
    35521 cgcccgacgt agagatcctg accactgtca aaagtgagtt caggaagaag tacaggggcg
    35581 ctctgacact taagattgtc actgatttcg gtgaacaggt gtatgacggc taccacgatg
    35641 tggccgtgaa aaagatgagc tcaggtaatt tcccgttcct ggtgaaaaac ctgaagccgg
    35701 ggttctataa ggcaacgctt gccctgcaga gcaagatggg caacaagaag tacagcttca
    35761 atttcggtta tgaaccggaa aagatagtgt cgccggcaga cccgcaacct gattttgcgg
    35821 aattctggca aagggcgaag gcggaacttg ccaccgttga tccccggtac aacctgatcc
    35881 ggatagacag cctctgcacg gagaagcatg atgtctacct ggttgagatg cgctcgctcg
    35941 gcaatgccct catcaggggc tggtaccggg tacctgtgaa acccggccgt tatcctgcaa
    36001 ttatgcaggt gccgggttac agctcggtgg aagtcccttc atatgtcaac tatggtgatg
    36061 atattatcgg attcgcactg aatatcaggg ggcacggcaa cagcaaggat gatgtgaacc
    36121 cgggctttcc cggatatata ctcagcaacc ttggagacaa ggaaaagtat atctacaggg
    36181 gagcttatat ggactgcgtc aggggtattg acttcctctg ctcgagaccg gaggttgatg
    36241 cctcgcgcat tgcagttgaa ggggcaagcc agggaggcgc ccttaccttc gccaccgcag
    36301 cgctggccaa tgaccggatc gcggtttgcg caccccaggt gccgttcctg tctgacttca
    36361 gggattattt cagggtggca aactggcctg gtaatgagtt catcgacctc gtcgaaaaca
    36421 aaaaagcgct gacatgggac caggcttact atacgctcag ttattttgac ataaaaaacc
    36481 tcgccccgat gataaaggct cccctgataa tgggagcagg actgatggat gaagtatgcc
    36541 cgccgcatat caacttcgcc gccttcaacc ttgtcactgc tgaaaaaaaa tatatcgttt
    36601 acccgtatgc cggccacggg ctccctgatc acttctacca ggcgaaaatg gactggatca
    36661 ggacaaagct gggattgaac taaaacatta ctgttatgga gggaagaaca aaaatgtcag
    36721 gactgcttat tgcggtcgga gttgctatct tgctgcaggg gtgttcacag cccgtgccgc
    36781 gtactcatgt cagtttcaac gatggatgga aatttgccct ggccgatgct gctgatgcct
    36841 cacaacccgg ttttgacgac agcggatgga ggaatcttga cctgccgcac gactggagta
    36901 ttgagggtga attcagcgag gcgaatcctg cctcgcccgg ggggggcgca cttccgggag
    36961 gagtgggttg gtaccgcaag accttcaggg taaacactgc ggataagggg cggatgactt
    37021 acatctcctt tgacggcgtc tatcgcaaca gcgaggtatg gatcaacgga aactatctcg
    37081 gcaagcggcc atacggctac agctctttca ggtatgacct cacccaatgg cttaagtacg
    37141 gtgaggagga aaacgtgatt gccgtcaggg cagacaactc cgcacagcca aactcaagat
    37201 ggtattcggg ctcaggcata taccgcaatg tctggctgac aaccactgaa aagattgccg
    37261 ttgaccagtg gggcacattt gtcaccaccc cggaggtcag cagtgaatcc gccctcataa
    37321 gggtgaccac ccagatcaaa aacagcaccg gctcgaaggc tgaagtaagg ctggagacca
    37381 ctgtatatga ctctgacaac aagcgcgttg tcagaacgga aactgaccag gtggaggtcc
    37441 ccgtatcagg tacagctgtg acacaggaac tgactgtcag gagtccccgc ctatggtcag
    37501 ttgacaatcc gcagctctac agggtggtaa ccaccatacg ctcaggccag cttcttgctg
    37561 acagatatga gacagttacc ggtataaggt catttgagtt tgaccgggtg aagggtttta
    37621 ttcttaacgg cgagcctgtg gtaatccgcg gagtatgcaa tcatcatgac ctgggctgcc
    37681 tcggagcagc ggtcaatacc cgtgcaatgg aaagacagct tgagatatta aaggaaatgg
    37741 gatgcaacgg catccgtacc tcccataatc ccccggcacc ggagctgctt gacctgtgtg
    37801 acaggatggg attcatcgtt atggatgagg cgtttgatat ctggaaagtg aagaagacag
    37861 acttcgatta cagtcttgat tttgatgaat ggcacaggag ggatattgaa gctatggtgc
    37921 ttcgtgaccg taatcatccg tcggtgttca tatggagcat cggtaatgag gtgatggagc
    37981 agtgggagag ggatggcagc ggtgaggcta tagccactga actggcaggc atagtcagat
    38041 cccttgatga caccaggccg gtgacggcgg catgcaatga tccggttccc tataacccgg
    38101 tcatcgcctc cggcgccctt gaccttatcg gttacaacta ccgcgacacg ctatggaccc
    38161 gcttcccgca ggcattcccg gaaggcaaat tcataggcac ggagaccact tcggccctgg
    38221 ccacccgtgg aagctatgac atgccgtcag agatagtaag gcgctggcct tcacgttggg
    38281 accagccctt cagggacggc aatgctgacc tcacctgctc ggcttatgac aactgctcgg
    38341 caccatgggg cacaacccac cgcgacagct ggaagctgat aagggacaat gcttttctca
    38401 ccggaatgta catatggaca ggctttgact acctcggcga gcctacaccg tactggtggc
    38461 cggcacgaag ctcttacttc ggcatcattg accttgccgg ctttccgaag gatgtctact
    38521 ggttttacca gagcgagtgg accgacaagg atgtgctgca tgtcttcccg cactggaact
    38581 ggaatcctgg tcagacggtt gatgtatggg cgttcaccaa ctgtgacgag gtggaactgt
    38641 tccttaacgg cagttccctt ggccggcaga gcaggagctg cgatgatttc aacctcgtgt
    38701 ggaaggtgcc gttcgaggag ggtaccctcc tgggcgtggg ttacaggaac gggagtgaga
    38761 ttatgagaag tgagatccgg actgccgggg aagctgctgc ccttcagctt tcgcccgaca
    38821 gggcagagat aatggctgac ggatcagatc tgtcattcat tacagtgacg gtcgctgaca
    38881 aagacggcaa tccgttgccg catgcctcta atttgatcaa cttttccgtg gggggcccgg
    38941 gcacgattgc cggtgttgac aacggaagcc agacaagcat ggaacccttt aaggcagatt
    39001 acaggaaagc atttaacggc aaatgccttg tggtggtgaa ggcagggaag gagaagggaa
    39061 cgataaagct caccgcctca tcggaaggac tgcccgatgc aaccgtaaca atgagggcca
    39121 ggtagaaatt tcttttcctg ctgcggctgc agcgggaagg tcagataaat accctcatcc
    39181 cctcatcgta gctctcccgg agctcacggg gg
//
LOCUS       DAOPXU010000011        39034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_912_length_39034_cov_11.474204, whole genome shotgun sequence.
ACCESSION   DAOPXU010000011 DAOPXU010000000
VERSION     DAOPXU010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 39034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39034
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_912_length_39034_cov_11.474204"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..2241
                     /locus_tag="PKK03_01950"
     CDS             112..2241
                     /locus_tag="PKK03_01950"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HNX83184.1"
                     /translation="MTTRNPEDSVHDEQFDGKEKTSESAEENAEMVDAVGQPAAESVT
                     ANGEDIPADAVPDKEEDVASDAVAEEAEEIASEALTDKEKEIASDAVAEEAEEIAAGD
                     AKAEEEAVPEESSPEGDAAETESVNYDEIELPPVDYSGFSRKELVETLALIVENRPPA
                     EIMDDVARIKEYFYRKTKAEFNEKRLNFAKEGGNIDEFRPEPDDLENRIKTILETYRS
                     RRSELNRIQESEKQDNLRKKVEIIEQIKDLVNREEAINKTFQEFRNLQNEWHSIGIVP
                     QTAVKDLWENYHHSVEIFYDYIKINRELRDLDLKKNLEAKIVLCEKAEELLLEPNAVN
                     AFKLLQDFHNQWREIGPVPHENKNDVWERFREATSKVNKRHHEFFEKQKDDQKHNLDA
                     KTALCEKVEEIAAGEILTFADFREKSEAILECQKLWKTIGFAPKKHNNKIYQRFRAAC
                     DTFFEKKRAFFAESKEVQVRNLQLKTELCLKAEALQESSDWKDTSEMLIRLQKEWKDI
                     GPVPKKYSEKVWKRFRKACDTFFTRKSEYFAGRDTSYEDNLKAKLAILDELEAFDPGE
                     DMRAGFDLLKDIQKRWTEIGYVPFNRKEEIARRYKEVLNRQFDKLKLDDEDKNILRYS
                     SKVDSAKSNPRAARKVRGEREKFYSKIKQLESDIVLWENNIGFFAKSANADTMIREVE
                     EKIAEARRNIKILEEKVKLIDSSMYEE"
     gene            2279..3883
                     /locus_tag="PKK03_01955"
     CDS             2279..3883
                     /locus_tag="PKK03_01955"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNX83185.1"
                     /translation="MTDVKYVFVTGGVTSSLGKGIISASLAKLLQARGYSVTIQKLDP
                     YINVDPGTLNPYEHGECYVTVDGAETDLDLGHYERFLNVHTTQANNVTTGRIYQSVIN
                     KERRGDYLGKTVQIIPHITDEIKRSIKIVGSGGNYEIVITEIGGTVGDIESLPYIESV
                     RQMRWELGPQNCVVIHLTLVPFLHASGELKTKPTQHSVKMLLEEGIQPDVLVLRTEHP
                     LTEDLRKKVALFCNVEPGAVVESIDVSTIYEVPLMMQKEMLDLTVLRKLGLSPGEEPS
                     LAEWKEFLHKLKNPAHRVRIALVGKYVELADAYKSISESFIHAGAANEAAAEVTMIHS
                     EEITDANYRTMLDGYDGIVVAPGFGSRGIEGKILTVNYARTNDIPFLGICLGMQCAVI
                     EFARNVLGLEGAHSTEINPKAKHPVIDLMEEQKSITEKGGTMRLGSYKCELLHGSEAW
                     KCYRKEEISERHRHRYEFNNKYLADFRNAGMIACGTNPESGLVEIIEIPSHRWFVGVQ
                     FHPEYSSTVIKPHPLFVGFVRACIRK"
     gene            3912..5897
                     /gene="yidC"
                     /locus_tag="PKK03_01960"
     CDS             3912..5897
                     /gene="yidC"
                     /locus_tag="PKK03_01960"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNX83186.1"
                     /translation="MDRNTVTGIILIFLIFMGFSLYNNHRMGKFFEAEKEYADSLYTA
                     GNYDQAREAYIRALNYKPKDQPTIQKLTDLNKQLGLGSVQQPDSAALTAGTTDTTAAL
                     QGSVPGRVTDSTAFGVFSSRTHGLDEFYTLENDLMVMKIAARGGKPYSVMLKKYSTHD
                     GRPVVLFDGDSTVFGYKFFTLDNKPIRTDELYFTPVGYQGPVVVSGSPASLTMRLEAS
                     NGAYIDFVYTMQPDDYMIGLESVFSSLSSVIPANTTSMALDWKMYIPQQEKGRQNEDM
                     YTGLKYKVYQEDVVDLGGTRMQRQKKDMVTESLMKTDWIAYKDQFFSSVLIADDFFLN
                     AAVSSTNADPTSRYLRFFESEIGVPYAHSDNSTVKMQFYFGPNSFTLMKKYDMQLEEL
                     VFLGRNIIRWINQFVIIPIFNLLDNFIVNYGLIILILTLIIKLVLFPLTHKSFISQAK
                     MRVMKPMVDEIAKKFPKQEDAMKKQQATMDLYKKAGVSPMGGCLPMLLQMPILFAMFR
                     FFPASIELRQQSFLWAQDLSTYDSILNLPFTIPMYGDHVSLFTLLMTASTIVTTKISS
                     PAGGQEQMPGMKTMMYIMPVMFMFILNNFSSGLTYYYFLANVISIIQTQFSRRFVSES
                     EILKKIEENKKKPVKKSKWQQRLEAAAKQSGRPLPKK"
     gene            5996..7036
                     /locus_tag="PKK03_01965"
     CDS             5996..7036
                     /locus_tag="PKK03_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNX83187.1"
                     /translation="MGKKVKNKDIAARLGLSGTLVSLVLNSKADQHGIKKETQERVLA
                     VARQMGYFNAVAEKGEPAPAEEKPGIIGMVVPSMNDPFIYEITPYLQKAFSSIGLGFS
                     VFTRDPDDMRFNRLTTSLKKFFSGMILVGNAADDSVVRALNRDSYPIVLLEKSIKRMR
                     LNTVCTDRIAGAAVMTQHLTSLGLKNLLIVSGEDTALYDKEMLEELRNSTKKSKNFDS
                     VHFTVAGLPKAGEKFDFQAIENFLRPPHRSDAIIVLNSALVFPLYLALQEKNIRIPQD
                     VALVSMEDGIGFDLLTTPVTRLRRPLPAMSLKVANIIWSEVKNQGKSKYKRQVNMSPE
                     LLVRSSCGSYLK"
     gene            7043..9667
                     /locus_tag="PKK03_01970"
     CDS             7043..9667
                     /locus_tag="PKK03_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNX83188.1"
                     /translation="MTRIIRLPALLSLAVLLSCSSGHLIEDKALRRAITDDYLARSAV
                     YSPVRPDLFGMTDTVSRTALREAVTFLLSYMPLSDLAVYEPGYLFENAAAALRARSEM
                     PWGGNVPPGIFLNFVLPPRVNNENPDHFRISCYAELKERVSGLTAMEAALEVNRWCHE
                     KVAYQAADSRTSAPLATMLSARGRCGEESTFTVSALRAVGIPARQVYTPRWAHTDDNH
                     AWVEFWVDGTWHYLGACEPEPVPDRGWFTEPARRAMLVHTKAFGRYDGSEKVIRKETL
                     FSEINTLGRYAETKELNIRVTDGNTVPVPGAMVGYMLYNYAEFYPLTRLQSDRNGRCS
                     LTTGYGTLLVWADDGDRYGFSLASPEDTLVHVAIFPPEAIEETLPLDIIVPEVPEPFP
                     GIDPALVQMNNALNHRGDSVRNAYISSWLAGVSTDEIARNTGLPSDAVSKILRTSMGN
                     YRAIAGFISGAGEKAGLALRILENISEKDLRDTPASVLADHLDNAPDPAPGSDIALYD
                     SYVLSPRISDELLSPFRSQLKALPGELTASFISDPAAAAAWIDTSITITNIENHYSVP
                     IIPGAVLRMRMADSHSRDIFFIALCRNAGVPSRFAPGTGRPQYHSAGEWHDVWFTGDN
                     RPSGTPGFLTFVPGHGSRMNQEPEYHVHFTLARIENGRFNTLDYGYGTKLSDMPVKIP
                     LDPGIYMLTTGNRDENGNVLASVSLLELKAGDEQQLTITPRDLPEIPVKSGKINLTTS
                     VKTFTGESVTLGSLAGKGLVLIWMEPGREPTRHLLNDLPGLKKEFDRWGGSFVFLTDP
                     ERTPGQITRESVTGAPENALFATDPGLGLLQSLSGNISRERSLPVVLYCDSGGEVLFS
                     SEGYRIGTGQQILKKIRE"
     gene            9674..11866
                     /locus_tag="PKK03_01975"
     CDS             9674..11866
                     /locus_tag="PKK03_01975"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNX83189.1"
                     /translation="MNKISFLVVAAVLAASSCSAPRQDDLTALVDPFIGTAAHGHVFP
                     GATTPFGMVQVSPDNGTSGWDWCSGYNYSDTVIAGFSHTHLSGTGIGDLCDILFLPSM
                     EGFNLSLPDSSASLAKLNRVWFSHDAEQSRPGYYAVALAPGVNAELTATPRCGLQRYT
                     AVTGGTMHVLADLGFAVNWDRPVGCLLAWDGTVLSGRRISAGWAEKQYLFFSSNFSKA
                     PASVTMLNAGAVAAGDRAEGRNIACILDFKMKEGEELIIKTGISAVDEAGAAGNLAAE
                     VPGWDFDGTAAAAQQAWQKRLGIISAESADSSLLKVFYTALYHTMIAPNLYTDADGRY
                     RGTDREIHNADGFTNYTVFSLWDTYRAAHPLFTLTAPDMVPDFVNTMLNIRKQQGKLP
                     VWSLAGNETDCMIGYHAVPVIADAVHKKFQGFDYPEALEAALESASLDFRGLNHYRNF
                     GYIPSELENESVAKTMEYAIDDWCISQMASDLGKPELAAEYLRRSHNYRNLFDTTIFF
                     VRGRMSNGSWRPDYDPLYSHHTRSDFTEGNGWQYTWLAPHDVDGLVSLFGSREMFLRR
                     LDSLFMVTEEVKGENSSPDISGLIGQYAHGNEPSHHIAYLFSMAGAPEKTESMVRRIM
                     AEMYTDKKDGICGNEDCGQMSAWYVFSAMGFYPANPADGKFILGSPVLDKAAISLPGG
                     KTFTVVSEGNSPGTVNVSACFLNGEKLDRNYITWKEITAGGELRFVMK"
     regulatory      11922..12135
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12238..14151
                     /locus_tag="PKK03_01980"
     CDS             12238..14151
                     /locus_tag="PKK03_01980"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNX83190.1"
                     /translation="MTVAVRAAAQDSFIEDTVNISAVTVTARASARLSPYTVVKMDAE
                     LLSRHEGGDLASLLQSSSNLFVRRNGNQGLASVSVRGMSGSHTLVTWNDLPLNSPGNG
                     FSDFAIIPLMAASTVRITSGGSDLDDISGYIGGKVELATDPEFDTGMTADVSLGAGSY
                     GELVSSASFTLGGPRFYGRLGAWSGKGTNDFRFINKDAPEGTRHERRSNASFASGGIM
                     GDLAYHKGNSRVSTHLWFNDADRELPGPVTTVQQDFGERQTDRSFRGVMKYSLDGDRL
                     TAGLTAGGSHDFNRYYHMVSSNNGDNSSSSLMLKMRLGYRVTGKTGLVLNAGDTYERA
                     SALNFESREERNAFSASVAVRYNPLPRLNLLLQVRQVAVTGVKVAPEFTAGASWMLSR
                     NGEHLLKMSLSRNTKLACFNDLYWIPGGNPDLEPETSAGGELSWSFAELRPSGDRNTL
                     DLSIYASRVEDLIQWIPGQSGLWHAVNLRSVNVSGLEARAGGQRSPGRWKLNGWMNYA
                     FTRSVIAGSDIANDRSVGSQLIYAPLHHANINLTAGREWLRTGLSAAWESRRFTTSDN
                     TEWLPSSIMTEAFAGAIFPVGPVKLQADLRVNNLFGIPVESVRNYPMPLRTFKIRLTL
                     TWSGKPNENENVL"
     gene            14135..15160
                     /locus_tag="PKK03_01985"
     CDS             14135..15160
                     /locus_tag="PKK03_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83191.1"
                     /translation="MKTYCKIITALTLLLASACEPQEKPASYPEGEGFFVLNEGNYLA
                     GNGTISFYSLETGEIYNDLFGTVNGRALGDIPSFMAVEGSMGYIVVNNSGTIEAIDMA
                     TMESLGTVTGLASPRQMVINEMKGYISSLQDGGIKVLDLDGMAVSDEIEVGSSTEAMV
                     ISAGRLFAAHWSAGSTITVTDLATGNLIRTISVGLEPESMVLDCNGKLWVLCTGGWQG
                     EEIPRIVRINTSTLEKEAELQFRTVDDNPSSLTCNAAGDTLYYLDEGVRRMQVTADAL
                     PSGVFIPAGDRLFYKLIAGPRGMVAVTDAIDYQQKGDLLIYNSRGELIDTEQAGIIPG
                     YMHYMKD"
     gene            15199..17373
                     /locus_tag="PKK03_01990"
     CDS             15199..17373
                     /locus_tag="PKK03_01990"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNX83192.1"
                     /translation="MKRLILIAIVSLSASLLVCGQPEFPSGSPLFPEIGKWHEISVDP
                     QHAEAMRAVNQPKDQPFRFAIPVEVSLTPFNSGFIATRGSETIWVLPVSSKGALSLNV
                     ILSPYNLPRGAYIYVYDHDRQIVRGAFTGESTSGTLSMPVMPVPGERLVLECHFPGTR
                     IPENSVGVTQVAHDFTGFFSPDGIKDFYYGHSDDCEIDLNCSTNPLYLTASRSVVRLL
                     VAGTELCTGVMVNNTGNVSRAYLITANHCIENQSHATNTVFVLNYRSPSCAGPDMTNT
                     YTLTGSELRATNPEIDFALVELNQFPSLVARPYFTGWDITATTPTNTFTLHHPEGDVM
                     KLSIDDNAPVTASYPVEGFVAAGFWRVLRWDLGTTERGSSGAPLFDQNGRLRGTLTGG
                     AATCDDPANDYYAKLIRMFNITTVSSGHLRPWLDPAGTGATLVSGRDPYGYNLSRSDT
                     IGGIPVNDPGTTDAYSSPGWGLATGNNSDVLTRYAEYIPFSGTGEIAWLRLQVSASSF
                     INVADSVRFYIWNGGALPGSVIATRKLRINEAVSGSVLEVDFGRTIKVTGSFYAGYTV
                     YYKNPLNQPQTQFAVARSAPWALPSQNTAYFYSGSAWKPFTQHPSFPMAVSLGIKAVM
                     VENTVLNQLEDPEQETTDLKVYPNPFASEVTFILTERGVTSTTLTIYDNVGRVVSTGE
                     YRNIFPGELTVPLPGLAPGIYHYGLRADSVFYSGTLVKTGTE"
     gene            17370..18506
                     /locus_tag="PKK03_01995"
     CDS             17370..18506
                     /locus_tag="PKK03_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNX83193.1"
                     /translation="MTRILVSVIAAALVLSSCRNAGNNQASGSGVDNPVAGRLTIEKE
                     KNYFRVTVSEPWQNSPGIQHVWYLIPRDSAVPSDIRETQTIRIPVRRMICMSTTHIAM
                     MRALGADSLIAGISGSSLIYDSVVLEGLRRGTIRDVGYEGSLNKELVISLEPDLLMAY
                     GVADPSTGNTSRLSETGVKVFYNADYLEEHPLSRCEWIRLFGLLTGREQRADSIFIRV
                     SGAYRELADKVRNGITSRPDVLLGAPWEDVWYVSPSDSYIGRLIEDAGGHYIYSDMTG
                     PNSVPFSVESVFRRAAEAELWINPGAAENLAGIAAADPRLASLPLFSEGKVWNNRRRV
                     TEEGGNDYWESAVVRPDLLLSDLVSIIHPELMPGYVQFYYRKLE"
     gene            18503..19537
                     /locus_tag="PKK03_02000"
     CDS             18503..19537
                     /locus_tag="PKK03_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNX83194.1"
                     /translation="MREGSTRIAVSMAGLFLLTLILFMADMLIGSTQMAASDVLKALF
                     SSGPAGDEIIVMQFRLPKALTAVLAGAAMSVSGLLMQTLFRNPLAGPDVMGVSSGAGL
                     GVALTLLTLTPLFTLAEGSILAGWGVILAAWAGAGAVMIIIMAVSARMRDIMTVLIVG
                     MLLASAISAVVSLMQYFSNEVMLRTYVIWTMGNLGNISYPQIRALALATAAGLAIAFT
                     MVKPLNAILMGETFSRTVGVRMQRTRIILLASASILAGSVTAFCGPLAFIGVAVPHIA
                     RLLFGTADHRILMPATALTGAVILLLSDIISVMPGGGHVIPVNTVTSLIGIPVVLWII
                     LGKAGLGRRF"
     gene            19537..20544
                     /locus_tag="PKK03_02005"
     CDS             19537..20544
                     /locus_tag="PKK03_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX83195.1"
                     /translation="MRRGGVIAETRDLEIGYMRKKTISVMKDLSLRARRGELVALIGR
                     NGTGKSTLLRTLVGLQPALAGEVMLDGRTLHETGSSQLPRIVSFVSTEPVAVRNIRVR
                     DVVALGRFPYTNWIGTRTESDNIAVIAALEATGIAALGDRSIDSISDGEKQRTLIARS
                     LAQDTGLLVMDEPTAFLDLPSRYGIVSLLRRLTREKGKCVIYSTHDLDTAINEADRIW
                     LMTGDGVTEGAPEDLMLTGSLARAFESPLLSFSVNDGTFSFIRGRQNTIALEGRGKAA
                     KLTTRALARCGYTTDPDAPRIVKIYERGHMTEWVVSDGSNEKRYGSLYDLVTDLPGND
                     Q"
     gene            complement(20538..21965)
                     /locus_tag="PKK03_02010"
     CDS             complement(20538..21965)
                     /locus_tag="PKK03_02010"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HNX83196.1"
                     /translation="MKFRLPNLILPLAFAALTTAASSQTGHSLTVTAQDAPSVKLRLA
                     YHVGNQQYVRDSLVTDSGGRGRFMGSERLPAGVYMIVLPDNLFFEFLIDEDQFFDIRF
                     SKSDPVATISFSGSEENTSFVAYQRKWKSLQEEAMKINGRLGGLQAGSPEASAARKEL
                     TDQEKKMKQYLKETAEANKGTLLGAIAWSVIPVETPAATVPPGTNNPDSVTRLISYLY
                     YKDHFFDHIDFSEPGLIRSPVLGGKLDQFFRQVVVQMPDSINKEADRLLEKSAVDSAM
                     FQYVAVWLMNRYASSEIMGHDAVVVHLADRVYLAGKAPWAAEEYVSDLRKRIERLRPN
                     LIGMKAPELLMGSFAGHYVSLYDVKADFTILYFWEPDCGHCKESTPLLKEYYDMNKAS
                     GIEVFAVCTHHDREKWERYIVDNGLTWINGWDPERMSRFDSLYNVESTPMVYILDRDK
                     KIIAKRLSVEDIPSFIDTYRKYQAH"
     gene            complement(22129..22830)
                     /locus_tag="PKK03_02015"
     CDS             complement(22129..22830)
                     /locus_tag="PKK03_02015"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNX83197.1"
                     /translation="MEITIKEFIKGAQEAQGLTVIIDVFRAFSTACYAFDSGINRLLD
                     VATPEEAFALRDKLKAEVIIAGEQDEKKPAGFDYGNSPTEMLTGSVAGKTMIHCTTAG
                     TKGIINAAGATEILGAGLVNAGAVASYISMIRPEKVTLVAMGYRALESAEEDMLCARY
                     IKDVLEGRESDISKETEALRKGSGSRFFNPANLDFSPPTDFFLCTDLNRFNFVLRAVI
                     TAGGYAEILRISMDH"
     gene            22916..24250
                     /locus_tag="PKK03_02020"
     CDS             22916..24250
                     /locus_tag="PKK03_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNX83198.1"
                     /translation="MRPVGEISQSIVSRAVQGRKAVAERLKKSVPFWIRQRRIIYLLY
                     KGIKRENIYIKASALTFFTILSVIPMVALAFGIAKGFGLSDELRAQIIMQFHNQEQVM
                     NWILDFANKTLEQTSGGWLAGIGVAFLFSTVGQLLRYVENTFNSIWKVDETRVWYRQV
                     TDYLAVIILVPALFVASSSATVLATTRLNDMLSQSDMLEGLKPVVSFLVQLIPFILLC
                     TLSTAAFLAMPNTRVKFRSALVAGLAAGIALQILQIVYVETQMGLTRLGTLYGSFAAI
                     PLLMVWVQMSWVVLLMGAQLSYYLQNITRYEFEFDVQTVSPRQKKRLSLLVMHSLIVD
                     FVKGAKPRAPEQISMELSLPVRTVHECLDLLHEASMVTEVWNEEQEKYVYQPATDINK
                     MTLSFVLEKLESSGSLHKIVINNSDYRKIDTALTRFESLVASSEVNILLRNL"
     gene            complement(24253..24954)
                     /locus_tag="PKK03_02025"
     CDS             complement(24253..24954)
                     /locus_tag="PKK03_02025"
                     /inference="COORDINATES: protein motif:HMM:NF016209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HNX83199.1"
                     /translation="MKTFLRRFENNRDLVILFGTWGIDEKAFSNLCTDNHDFILFYNY
                     SADEPLILPEMKTYRRVTVIGWSLGVWAAEYYSRKLGIKPDLTIAINGTPVAADDKYG
                     IPLRIFEATLENITNYSMGKFYLRLFGTRSQFELNRAYIPTRTEKSLHDELRWLYNRM
                     MEPVETGFVWDYSLIGSEDRVFRASNLTSYWSMHPETRQIMVEAPHYLFDNWNSLEEL
                     IRYVRRFRLSPFRNK"
     gene            25308..26156
                     /locus_tag="PKK03_02030"
     CDS             25308..26156
                     /locus_tag="PKK03_02030"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNX83200.1"
                     /translation="MIPEIISIRHDDGSGFRAAWMDNESGFLHLHPEYEIVLNISGNG
                     TRVAGDSVELFDRYDLVMYGPDLRHFWNFYRKDENETGRHAIMVHFRRDSLGDSMLSQ
                     FEMADLRRLLENAGRGIAFPPETGEAAEEPMRRLLSDTGMKKMVSFFRLLEILCAAPR
                     FKILSGENREGVHEITDSRLTEVCSFVRDNFYRPIPVKEVAVVAKMSPHSFSRFFSRS
                     TGSGFVDYVNQVRTNKACYLLRETDHQINEIVFECGFATVSNFNKQFRKHAGMSARDY
                     RAQYRN"
     gene            26266..28080
                     /locus_tag="PKK03_02035"
     CDS             26266..28080
                     /locus_tag="PKK03_02035"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HNX83201.1"
                     /translation="MGMTITEKIIAAHSKYKSVKPGDIVDITIDARAARDFGGANVVK
                     NLNDFGLKVDDPEKTLFTFDCNPTGSDQKYAVNQHICRQYARGNGIKVYDIDAGIGTH
                     LLIEEGYVWPGATAVSTDSHANILGAVGAFGQGMGDMDIAAAWNSGRVWFKVPASVKL
                     TLKGRMPEGVAAKDVILNLLRMYGANTLLGYSIELYGDAVERMSLDERITIASMATEM
                     GAIIILFPPTPAIIGYCRARARAPFEPVHADADATYAITADIDVSTFVPMVARPGEPH
                     DTVGVSQLPVTRIDSAFIGSCTNGRIEDMRATAEILKGRKVAPGVVLKIVPSTDAVWK
                     QCLDEGLMDIFKDSGALVSNAGCAGCAAGQVGQNGKGEVTVSTGNRNFPGKQGQGSVF
                     LASPAVVAASAIAGYITTPEMIPVTPPEFKFSATQPSAAAPVKSTQTDRPTVIRGKVW
                     LIERDNIDTDMIFHNRYLAITDIREMGQYAFDNLEGYRDFAKKAQAGDIIIAGKNFGS
                     GSSRQQAVDCFVSLGIQAVVAQSFGAIYERNAINAAFPVLTYSSFEKIDLRDQDVISV
                     NILTGEAVNERNGLSTLINPFYDAQYEIYKKGGLLGKI"
     gene            28099..29874
                     /locus_tag="PKK03_02040"
     CDS             28099..29874
                     /locus_tag="PKK03_02040"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HNX83202.1"
                     /translation="MKPRTFVEKVLGAPEGAVVFRKPDLILTHDNTSSIFQTFRKMGG
                     EKVSDPDQMVIVLDHNAPPADAKLATQYQDIRNIVAGQGIRKFYDSGMGICHQMMSNH
                     ARPGMIIVGSDSHTCTAGAFNALAAGIDRTEAAGIWKRGETWFLVPPSMRIDLTGRLR
                     PGVSAKDLSLWIIGMLGSDGANYMSIEFHGDGVASLSVSERMTVANLASEMGAKNAVF
                     PDDEVLQKFIGSNSPGIWADEGAEYKSRISIDLSKVFPLVAAPHHVDNIKSVASVAGT
                     VVHEGLIGTCTNGRIEDLRTAASVLRGAQVKPGFQLHIIPASKEIYLQAIREGIVTDL
                     IEAGANILSPSCGPCLGTGQGIPASGHTVISTANRNFLGRMGNKEASIYLASPATVAA
                     SAVTGVITDPRVTRGDEVFPFNPPQSQTNTIREGENRKEGNVWNYADVDNLNTDQMFA
                     GKHTYSVMSSDAAAILPLLFGDFDPSFSANAKAGDTIIAGDNFGCGSSREHPSVGLAH
                     LGIRAVIIKSVNRIFYRSSINQGLPLIVHREAVEAYRAGDAVEIDFDRGMLKVGEKQF
                     RFEPLPPELKAIIEKKGLVNWMKTA"
     gene            29956..30029
                     /locus_tag="PKK03_02045"
     tRNA            29956..30029
                     /locus_tag="PKK03_02045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29990..29992,aa:Arg,seq:tct)
     gene            30057..31520
                     /locus_tag="PKK03_02050"
     CDS             30057..31520
                     /locus_tag="PKK03_02050"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNX83203.1"
                     /translation="MTEEGGMNTISFRSVLKISGLILLISASSFLLCIPVALIYSEPV
                     SPFLLAFVTALTPGALLYFLINSPLHQKVTSREGYLSVTLGWIMLVISGTFPYIFSHE
                     LKGFVNMLFESVSGYTTTGASILTEVESLSKSVLFWRSLTHWIGGIGIILLVIIVLPS
                     LKVGGYSLFSLESSLKEKILPRTKSIAITVLMIYLLLTALLVILLTAGGMNLFDSICH
                     SFGTVATGGFSTKNTSIAGYSGYIQYVLGIFMFLSAASFVVFYFILKRNFSRVKANEE
                     LWFYLFFTTASVVAVTLVLYTGTERNFEVAFRHAFFQVTSTISTTGFATTDYNVWPQA
                     ALVMIFLLMFAGGSTGSTTGGIKMARHLIALKNLRNVTVRLLHPSAVIPVKLNGQVVP
                     ESINSLMTVFIQLYVIIFIAGTLIILVSGIPAIEAAGASVSALSCVGPGFGASGNMGN
                     YAHFNAVAKLTMVVLMIIGRLEIFTILALFTRTFWKK"
     gene            31618..31809
                     /locus_tag="PKK03_02055"
     CDS             31618..31809
                     /locus_tag="PKK03_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNX83204.1"
                     /translation="MNKGIVKFFNVSKGFGFIKDANSSKEYFVHSTGLIDKIKENDEV
                     TFDLQEGKKGLNAVNVRLA"
     gene            complement(32022..32807)
                     /locus_tag="PKK03_02060"
     CDS             complement(32022..32807)
                     /locus_tag="PKK03_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNX83205.1"
                     /translation="MARSVNEMLCCPSCKGELIHQADSKADRLNHDLLSCSKCDVSYR
                     HFGGYYDFLGGEGLVFKNRREKFFRSVLAKVYTPLTNMMFLLCGGPKSARNEVMGRLR
                     VNDGDTVLETGIGYGENLLWLARRSAGLQLYGLDIQEEMMSHCVSNLNRWKINSELVR
                     ADAQKLPYRDNVFDVVFHLGAINLFEDKGLAIREMIRVAKPGTHIVIADETEKAGRLF
                     NIFTGSAEKVVPPVDLVPAGMKNIALSTIWRGYGYVIEFDVAK"
     gene            32978..33934
                     /locus_tag="PKK03_02065"
     CDS             32978..33934
                     /locus_tag="PKK03_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNX83206.1"
                     /translation="MKVLVTGSSGYLGSIAVSHLVAKGCEVIGLDMKKPDGKVDGDGF
                     RFYECNSTDGSCVRDIFSRELPDAVLHFACSMNRLRNRKEEFGMDVGGSFNVIEAARR
                     TPSVRKFVFSSSAAIYGPAARSELWLDEAEPLNPGHYTYGLNKRLIEQMLFRVNGDHP
                     MRTVSLRICTVVGPRYSRPKSVVSILLRLPFLPESFSRTSVQFLHEDDFNELLNLVLH
                     DSEIEGVFNLAPDSCSVVGEIVPPARFHRFPCSAIKPVMWALWNLRMVNLQPAGLGFC
                     LYPVVLDSKRLARRYDYRFRHSSTESFLLTKERNSLPPVARF"
     gene            complement(33938..34576)
                     /locus_tag="PKK03_02070"
     CDS             complement(33938..34576)
                     /locus_tag="PKK03_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HNX83207.1"
                     /translation="MVNRYIITIGRQLGSGGREIGQKLSARLGIAFYDKELIRIASQQ
                     SGLKEEFFERVDEQKHFSLFPGILGLRTSLTDDFFSNYYLSNESLFRIQSDVMRTLAG
                     EGSCIFVGRCADYVMKDERNCLNLFISADMADRIKRIAISHKLSDTKAKELIERTDKG
                     RSSYYNYFSGKTWGAAESYHLCINSSVLGIDETVRLISHVAGSRFGLTENRE"
     gene            34738..36240
                     /gene="glpK"
                     /locus_tag="PKK03_02075"
     CDS             34738..36240
                     /gene="glpK"
                     /locus_tag="PKK03_02075"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNX83208.1"
                     /translation="MKKKYILSLDQGTTSSRAILFGSDGQPVASSLKEFTQIFPRPGW
                     VEHDPAEIWSTQAGVAAEVVAGSGADCSDIAAIGITNQRETTVVWNRRNGKPVYNAIV
                     WQDRRTADLCDKLRAEGHGAMIAEKTGLVIDAYFSATKVKWILDHVDGARAAAERGDL
                     AFGTIDSWLVWNLTGGRLHVTDVSNASRTMLFNIRSLQWDDVLLSLFEIPSSMLPEVR
                     SSSEVYGYSTGLFASGIPVAGIAGDQQAALLGQMCVDEGMVKNTYGTGCFMMMNTGSE
                     PMRSGNRLLATVAWKIGDDTTYALEGSIFIGGAVVQWLRDGLGIIRQSSEVERLASQV
                     SSAEGVCFVPAFAGLGAPYWNQHARGTITGLTRGTTAAHIARAALESIAFQTLDVLLA
                     MEKDSNLKIKELRVDGGATVNDSLMQFQADILQTDVLRSGVTETTALGAAFLAGLATG
                     FWGGMDELRGLWQAGRHFAPAMKPEERKTLIQEWNRAVMTAGAWADDAGR"
     gene            36289..37443
                     /locus_tag="PKK03_02080"
     CDS             36289..37443
                     /locus_tag="PKK03_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNX83209.1"
                     /translation="MKKTFLLAALMMLIISPGLKAQNADSADVYMITCAPGTAAYSIY
                     GHTALRITMRGTEFDMVYNWGIFDFSTPNFVYRFAKGRLDYKLGAYSYARFLEEYISE
                     GRSVWSQKLNLTTAEKERLFILINENLRPENIKYRYDFFFDNCATRVRDIVAASAADT
                     VIFPVKEKRLSFRQLIDPYQKVLPWLDFGADMLLGLQADRKASVADEMFLPVYLRDNF
                     AGSLIVHDGRQEPLAGPTEKVVDRPVTQTGKKAWVPQAVFWLIFVFVVLVTFVFKRPG
                     LEKVLDYILYFIFALIALLLIFTNIFSEHDALHLNLLFLGINVLITVLFIMMLAGKKS
                     VKLSRLTLTISVLWFPVALIAGQGINPALIPLVLTIMVRLFRHCGFGKSL"
     gene            37536..38531
                     /locus_tag="PKK03_02085"
     CDS             37536..38531
                     /locus_tag="PKK03_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNX83210.1"
                     /translation="MEHTTDIRLLNEKIEKESAFVDLVRQEINKVIVGQRHLTDSLLI
                     GLLSNGHILLEGVPGLAKTLAIKTLASIIDTKFSRIQFTPDLLPADLIGTMIYSQRKE
                     EFVVRKGPIFANFILADEINRSPAKVQSALLEAMQERQVTIGDTTYRLDEPFMVLATQ
                     NPIEQEGTYPLPEAQVDRFMLKVVITYPEKEEERLIIRENLASEFPVAKTILKPEDII
                     RARSVVREVYIDEKIENYILDIVFATRQPDKFGLSKFTPLIAYGASPRAGINLALAAK
                     SYAFIRRRGFVVPEDVRSLCADVLRHRIGLTYEAEAENITQDQIIAEVLNTVEVP"
     gene            38536..38940
                     /locus_tag="PKK03_02090"
     CDS             38536..38940
                     /locus_tag="PKK03_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83211.1"
                     /translation="MKRLQLSLILLTFLIPVKAQEFCGMTEKSIRAVMERDFKGLTPD
                     DNVRNDVYRYLKYHSVDDNETWLIFLDERNRCKGVRITYSNSLYDTKISELNRKYGHD
                     DGGKWSYRLERNRIAVTVHRDEWFFTVTHVRM"
BASE COUNT         9717 a         9604 c        10356 g         9357 t
ORIGIN      
        1 gggtattggt ttgagtttta ccaataatcc attattttcg tggtttgaaa aaaagggggg
       61 aggttcatcc tatcctcctt tgtatgaagc actgaagaaa aaggattaat gatgactaca
      121 agaaatccgg aagactctgt tcatgatgaa caattcgatg gtaaagaaaa gaccagcgaa
      181 tctgcggaag agaatgctga aatggttgac gcggtcgggc agcctgccgc tgaatccgta
      241 accgcaaatg gggaagatat tccagccgat gccgtacctg ataaagagga agatgttgcc
      301 tccgatgccg tagctgagga agctgaagag attgcttccg aagctctgac tgataaagag
      361 aaagaaattg cctccgatgc tgtggctgag gaagctgaag agattgccgc cggcgacgca
      421 aaggcagagg aagaagctgt tccggaggaa tcatcgcctg aaggcgatgc agctgaaacg
      481 gagtcagtca actatgatga gattgagctt cccccggtag actacagcgg attctcaagg
      541 aaggaactcg tggagacgct tgccctcatt gtcgagaacc gtcctcctgc cgagatcatg
      601 gatgatgttg cccggattaa ggagtacttt tacaggaaga caaaggcgga attcaatgag
      661 aagaggctga attttgcaaa ggagggcggg aatattgatg aattcaggcc tgaacccgat
      721 gatctggaga acaggatcaa aaccatcctt gaaacctacc gcagccgcag aagtgaactt
      781 aacaggatac aggagagtga gaaacaggac aatctgcgca aaaaggttga aataatagaa
      841 cagataaaag atctcgtgaa ccgtgaggag gctatcaaca agaccttcca ggagttccgg
      901 aaccttcaga atgaatggca tagcataggc attgtgccgc agacagccgt caaggatctg
      961 tgggagaact atcaccacag tgtcgagatc ttctatgact atataaagat taaccgtgag
     1021 ctgagagacc ttgacctgaa gaagaacctt gaggccaaga ttgttttgtg tgaaaaggct
     1081 gaggaactgc tccttgaacc caatgcggtc aacgccttca aactattgca ggatttccac
     1141 aatcagtggc gtgagatcgg tccggttcct catgaaaaca agaatgacgt ttgggaacgt
     1201 ttccgcgaag caacctcaaa ggtaaacaag aggcatcatg agttcttcga gaagcagaag
     1261 gatgaccaga aacacaacct cgatgccaag accgcacttt gtgagaaggt tgaagagata
     1321 gcagcaggag agatacttac ctttgccgac ttcagggaga agtcagaagc tatacttgaa
     1381 tgtcagaagc tgtggaagac tatcggattt gctcccaaga agcacaacaa caagatttac
     1441 cagcggttca gagcagcctg cgacaccttc tttgaaaaga agcgggcatt ctttgccgag
     1501 agcaaggagg tccaggtaag gaacctccag ctcaagactg aactctgtct caaggctgaa
     1561 gcactgcagg agagcagtga ctggaaggat accagcgaga tgctcatccg gctgcagaaa
     1621 gagtggaagg atatcgggcc cgtcccgaag aaatactccg agaaggtatg gaagcggttc
     1681 cgcaaggcct gtgacacctt ctttacacgg aaatccgaat acttcgcagg gagggatacc
     1741 tcctacgaag ataacctcaa ggccaaactg gcaattctgg atgagcttga ggcatttgac
     1801 ccgggtgagg atatgagggc tggtttcgac ctgctcaagg atatccagaa aaggtggaca
     1861 gagatcggat atgtgccctt caacaggaag gaggagatag cccgcagata caaggaggtt
     1921 ctcaacaggc aattcgacaa gctgaagctt gatgacgagg acaagaatat ccttcgctac
     1981 agcagcaagg ttgacagtgc aaaatcaaat cctcgcgcag caaggaaagt gaggggtgaa
     2041 cgggaaaaat tctacagcaa gatcaaacag cttgagagcg atatagttct ctgggagaat
     2101 aacataggtt tctttgcaaa atcagccaac gctgacacaa tgatcaggga ggtggaagaa
     2161 aagatagccg aagccaggcg gaatatcaag attcttgagg aaaaggtcaa gctgattgac
     2221 agttcaatgt acgaggaata gtaattccca ttcagccaat aacataagag gataagcagt
     2281 gacagacgtt aagtatgttt ttgtaaccgg cggggtcacg tcatcgctgg gcaaaggtat
     2341 catttctgcc agcctcgcga agctccttca ggcgcgcggg tattctgtga ccattcagaa
     2401 gcttgatcct tatataaatg tcgacccggg aacccttaat ccctatgagc atggcgagtg
     2461 ctatgtcacc gttgacggcg ccgagaccga tcttgacctc ggtcattacg aacgtttcct
     2521 gaatgtccac accacccagg caaacaacgt caccacaggg aggatatacc agtcggtaat
     2581 aaacaaggag cgccggggag attatcttgg aaagaccgtg cagatcatac ctcacatcac
     2641 tgatgagatc aagcggagca ttaagattgt gggttcaggt ggcaactatg agattgtcat
     2701 cacggagatt ggcggaacgg tgggtgacat tgaatcgctt ccctatattg agtcagtaag
     2761 gcagatgcgg tgggagctcg gtccccagaa ctgtgtggtg atacacctga cgctggtgcc
     2821 attcctgcat gcttccggtg agcttaagac gaagcccacg cagcattcgg taaaaatgct
     2881 gctggaagag gggatacagc ctgacgtgct ggtgcttcgc accgaacatc ccctgactga
     2941 ggatctgagg aagaaagtgg ccctcttctg caacgttgaa cccggcgccg tggtggagtc
     3001 aattgatgtc tccaccatat atgaggtgcc tctcatgatg cagaaggaga tgcttgacct
     3061 gacggttctg cggaagcttg gcctgtctcc gggcgaggaa ccttcccttg cagaatggaa
     3121 ggaattcctg cataagctca agaaccctgc gcaccgggtg aggattgcgc tggtcggcaa
     3181 gtatgttgag ctggctgacg cctataaatc catctccgag tcgtttatcc atgccggggc
     3241 cgcaaatgag gccgctgcgg aagtgacaat gattcattca gaggagatca ctgatgccaa
     3301 ttacaggaca atgcttgatg gctatgacgg cattgttgtg gcaccggggt ttggttctcg
     3361 gggcatagag ggcaagattc ttacggtgaa ttatgcccgc accaatgata ttcctttcct
     3421 gggaatatgc ctgggtatgc agtgtgctgt gattgaattt gcaaggaatg ttctggggct
     3481 tgagggggct cattccaccg agatcaatcc caaggcaaaa caccctgtca ttgacctgat
     3541 ggaggagcag aaaagcatta cggagaaggg tggaaccatg aggctgggaa gctacaaatg
     3601 cgaactcctt cacggatctg aagcctggaa gtgctacagg aaggaagaga tatctgaacg
     3661 tcaccgtcac aggtacgagt tcaacaataa atacctcgcc gacttccgca atgcaggaat
     3721 gattgcatgc ggcaccaacc ctgagtcagg cctggtcgaa atcattgaga taccgtccca
     3781 caggtggttc gtgggagtgc agtttcatcc agagtacagc agcactgtga ttaaacccca
     3841 ccctctgttc gtcggatttg tcagggcatg tatcagaaaa taataacaat aaaacaggga
     3901 ataaaaaaat aatggataga aatacagtaa ccggcatcat cctgatcttc ctgatcttta
     3961 tgggattcag cctgtacaac aatcacagga tggggaaatt cttcgaggct gagaaggaat
     4021 atgccgattc tctttatacc gccggtaatt atgatcaggc ccgagaggca tatataaggg
     4081 cgcttaatta caaaccaaag gatcagccta caattcaaaa gctaacagac ctgaacaaac
     4141 agctgggact gggaagcgtg cagcagcctg attcagccgc tctgactgcc ggaacaacag
     4201 atactacagc tgccctgcag ggttcagtgc ccgggagggt gactgacagt acggcattcg
     4261 gtgtctttag ttccagaact cacgggctgg atgagtttta tacccttgag aatgacctca
     4321 tggtcatgaa gattgccgcc aggggaggca aaccctattc agtcatgctg aaaaaataca
     4381 gcacgcatga cggtcgtccg gttgtgcttt ttgacggcga ctccaccgtg ttcggctaca
     4441 agttttttac acttgacaac aaacccatac ggactgatga gctttacttc accccggtgg
     4501 gttaccaggg accggtggtt gtcagcggaa gcccggcaag cctgaccatg aggctcgagg
     4561 catcaaacgg cgcatacatt gattttgtct acactatgca gcctgatgat tacatgatcg
     4621 ggttggaatc ggttttcagc tcactctctt ctgtcattcc ggccaatacc acatcgatgg
     4681 cgcttgactg gaagatgtat atcccgcaac aggagaaggg caggcagaat gaagacatgt
     4741 acacgggtct gaagtacaag gtctaccagg aggatgtggt ggaccttggc ggcaccagga
     4801 tgcagagaca gaagaaggat atggtcaccg agagcctgat gaagacagac tggatagcat
     4861 acaaggatca gttcttttca tcggtgctta tagctgatga tttcttcctc aatgcagcgg
     4921 tctcttccac caacgctgat cccacctcca ggtacctgag gttcttcgag tcagagattg
     4981 gtgtgcccta tgcccacagt gacaacagta cggtgaagat gcagttttat ttcggtccca
     5041 acagcttcac cctgatgaaa aagtatgaca tgcagcttga agaactggtt ttcctgggca
     5101 ggaacataat caggtggata aaccagtttg tcatcatacc gatcttcaac cttcttgaca
     5161 actttattgt caactacgga ttaataatat tgatcctgac actgattatc aagctggtcc
     5221 tctttccgct tacgcacaag tcattcatca gccaggccaa gatgagggtg atgaaaccaa
     5281 tggtggatga aattgccaag aagtttccga agcaggagga tgcgatgaag aaacagcagg
     5341 cgacgatgga cctgtacaag aaggcagggg taagtcccat gggcggctgt cttccgatgc
     5401 ttctgcagat gcctatcctg tttgccatgt tccgcttctt ccccgcctcc atcgagctga
     5461 ggcagcagtc attcctctgg gcccaggatc tctcgaccta tgacagcatc cttaatctgc
     5521 ccttcaccat cccgatgtat ggtgatcatg tgagtctctt cacactgctc atgacggctt
     5581 cgacaattgt caccaccaaa ataagcagcc ctgcaggagg gcaggagcag atgccgggga
     5641 tgaaaacgat gatgtacata atgcccgtaa tgttcatgtt tatattgaac aatttctcgt
     5701 cgggtctgac atattattat ttccttgcaa acgtgatttc gataattcag acacagtttt
     5761 cacggcggtt tgtaagtgaa tcagagatac ttaagaagat agaggaaaac aaaaagaagc
     5821 cggtcaaaaa gtcaaaatgg cagcagcgtc ttgaagctgc ggcaaaacaa agcggacgtc
     5881 cattacctaa aaaatagcgg caaacattgc ttttgaacat ttttttaaca aatatgctct
     5941 aaagcatcca tttctaaaaa tataaggcta attttgagct ttaattttaa atgaaatggg
     6001 gaaaaaggtt aaaaacaaag acattgccgc aagactaggt ctttcaggca cgttggtatc
     6061 attggtgctg aacagcaagg ctgatcagca cggaatcaag aaagagacac aggagagggt
     6121 tctcgcggta gcgagacaga tgggttattt caatgctgtt gcagaaaagg gggaacctgc
     6181 accggctgag gagaagcccg ggattatcgg tatggtggtg ccctcaatga atgatccgtt
     6241 catctatgag atcactcctt acctgcaaaa ggctttctca agtatcgggc ttggtttttc
     6301 agtattcaca agagatccgg atgatatgcg cttcaacaga ctcaccacct cgctgaagaa
     6361 gttcttcagc ggtatgatcc tggtgggaaa tgctgccgat gacagcgttg tcagggctct
     6421 gaacagggac tcctatccga tagttctgct ggagaagagt ataaagagga tgcggcttaa
     6481 caccgtctgc actgaccgta ttgcaggcgc tgcggtgatg acacagcatc ttacctcgct
     6541 ggggctgaag aatctgctga tcgtctcggg agaggacact gccttatatg ataaggagat
     6601 gcttgaggag ctcagaaatt caacaaagaa atccaagaac tttgattctg tccacttcac
     6661 cgtggcaggg ctgcccaaag caggggagaa gtttgacttc caggccatag aaaatttcct
     6721 gaggccgcct cacaggagcg acgcgatcat tgtcctcaac tcggcactgg tcttcccgct
     6781 ttaccttgca ctgcaggaaa agaacatccg catacctcag gatgtggcgc tggtatcgat
     6841 ggaagacggc attggcttcg accttctgac cactcccgtc accaggctgc gcaggcctct
     6901 tccggccatg tcgctcaagg tggcaaacat catctggtca gaggttaaaa accagggtaa
     6961 aagcaaatat aagcgtcagg tcaatatgtc tcctgaattg cttgtcagga gcagttgcgg
     7021 ctcttattta aaataatcca taatgacacg aatcatcagg ctgccggctt tattgtcgct
     7081 ggcagtgctg ctttcctgca gcagcggaca tcttattgag gataaagcac tgcgaagggc
     7141 gattactgat gattatcttg ccaggtcagc ggtgtactct ccggtacgcc ctgacctctt
     7201 tggcatgact gatactgtca gcaggacagc gctgcgtgaa gcagtcacct ttctgctttc
     7261 atacatgcca ctgagcgacc tggccgttta tgagcccggt tacctgtttg agaatgcggc
     7321 agctgccctc cgtgcgcgga gtgagatgcc ctggggcggc aatgttccgc cgggcatttt
     7381 ccttaatttt gtgctccccc cgagagtcaa taatgaaaat cctgaccact tcaggattag
     7441 ctgttacgca gagcttaagg agcgtgtcag cggactgacc gcaatggagg ctgcccttga
     7501 ggtcaaccgc tggtgtcatg agaaggtagc ctaccaggca gccgactccc gcacctcggc
     7561 gcctcttgcc acaatgcttt cagcccgtgg cagatgcggg gaggaatcta ccttcaccgt
     7621 atcagcatta cgggccgtgg gtatccctgc ccgccaggtc tacaccccca ggtgggccca
     7681 taccgatgac aatcatgcct gggtggagtt ctgggttgac ggtacatggc attaccttgg
     7741 cgcctgcgag ccggagcctg ttcctgacag gggttggttc accgaacctg cgcgaagggc
     7801 aatgcttgtg cataccaaag catttggcag gtatgatggc agtgagaagg tgatcaggaa
     7861 ggaaacgctt ttttctgaaa ttaataccct cggcaggtat gctgagacaa aagaactgaa
     7921 tatacgggtc actgacggaa atacagttcc tgtgcccgga gcaatggtcg gatacatgct
     7981 ctacaactac gccgagtttt atcccctgac aagacttcag agcgacagaa acggcaggtg
     8041 cagcctgacg acgggttacg gaacattgct tgtatgggct gatgacggcg acagatacgg
     8101 attctccctc gcttcacccg aggacacgct tgtgcatgtg gccatctttc ctccggaggc
     8161 aattgaggaa acccttcctc ttgacattat agtgcctgag gtgcctgagc ccttccccgg
     8221 cattgatccc gccctggttc agatgaataa tgcacttaac cacaggggag acagcgtaag
     8281 gaatgcatat atcagctcct ggcttgccgg tgtcagtact gatgagattg cccgcaatac
     8341 aggtttgcca tccgatgcgg tcagcaaaat actcaggaca tcaatgggca actacagagc
     8401 tattgccgga tttatttccg gtgccggtga aaaggccggg ctggcgctcc gcatacttga
     8461 aaatatttcg gaaaaggatt tgcgtgacac tcctgcatcg gtgcttgccg atcatcttga
     8521 caacgctcct gatccggctc ccgggagtga catagcactg tatgacagct atgttctctc
     8581 gcccaggata tcagatgaac ttctgtcacc tttcaggagc cagcttaaag cactgcccgg
     8641 cgaactgacc gcatcattca tcagtgaccc tgctgcagca gcggcctgga ttgacacttc
     8701 catcaccatt acgaacattg agaaccacta ttcagtgcca atcatccctg gcgctgtgct
     8761 cagaatgcgg atggctgaca gccattcacg ggacatcttc ttcattgcac tgtgcaggaa
     8821 cgccggcgta ccttcacgct ttgcgccggg aaccggaaga ccgcaatatc atagtgccgg
     8881 agaatggcat gacgtatggt tcaccggtga taaccgaccc tcggggacac ccggcttcct
     8941 gacttttgtt cccggccatg gcagcaggat gaatcaggaa ccggaatatc atgtccactt
     9001 cacacttgcc aggattgaaa acggaagatt caataccctt gattacggat acggaacaaa
     9061 gctcagcgac atgccagtta aaattccgct cgatccgggt atttatatgc tcactacggg
     9121 aaacagggat gagaacggaa acgttcttgc ttcggtctcg ttacttgaac tgaaggccgg
     9181 agacgaacag caactgacca ttaccccaag ggatcttccg gagatccctg tgaagagcgg
     9241 gaagataaac ctcacaacct cggtgaaaac attcaccggt gaaagtgtaa ccctcggttc
     9301 cctcgcaggg aagggtcttg tcctgatctg gatggaaccg ggaagggaac ccacccgcca
     9361 tctgctgaat gaccttcccg gactgaagaa agagtttgac agatggggcg gatcttttgt
     9421 atttcttact gacccggaga gaaccccagg acagattacc cgggagtcag tcaccggagc
     9481 gcctgagaat gctctttttg caactgaccc ggggcttggg ctgttgcagt ctctgtccgg
     9541 gaacatatcc cgtgagaggt cattacccgt ggtgctgtac tgtgacagcg ggggagaggt
     9601 gctcttttct tcggaaggat acaggatagg tacaggccag cagatattga agaagatcag
     9661 agaataattt atcatgaata aaatcagctt tctcgttgtg gctgctgttc ttgcagcctc
     9721 gtcatgttca gcgcccagac aggatgacct gaccgctctg gttgatccgt ttataggcac
     9781 tgccgcccac ggtcatgtat tcccgggggc cactacccct ttcggaatgg tgcaggtcag
     9841 tcctgacaac ggaacctcag gatgggactg gtgttcggga tacaactact ctgacacggt
     9901 gattgccgga ttcagtcata cccatctcag cggcaccggt atcggcgacc tgtgtgatat
     9961 cctgtttctg ccgtccatgg agggattcaa tctttcactg cctgacagca gtgcctctct
    10021 ggcaaagctc aacagggtct ggttctctca tgatgccgag caatcaagac cgggatacta
    10081 tgccgtggct cttgctccgg gggttaacgc cgaactcacc gccacaccac ggtgcggttt
    10141 gcaaagatat acagctgtta ctggaggaac catgcatgtg cttgcagacc tgggttttgc
    10201 cgtcaactgg gaccgccccg tcggttgcct gctggcatgg gacggcactg ttctcagcgg
    10261 caggcgcatc tctgcaggat gggctgagaa acagtatctg ttcttctctt caaatttttc
    10321 aaaggcgccc gcctcggtta caatgctcaa cgccggagcg gttgccgcgg gtgacagggc
    10381 agagggcagg aacatagcat gcatccttga ttttaagatg aaggagggtg aggagcttat
    10441 aattaaaacg ggcatctctg ccgttgacga ggcgggcgct gcaggcaacc ttgccgctga
    10501 agttcccgga tgggacttcg atggtaccgc tgctgcagca cagcaggcat ggcagaagag
    10561 gctcggcatt atcagtgccg aatcagctga cagttcactt cttaaggtct tctacaccgc
    10621 cctgtatcat acaatgattg caccgaatct atacaccgat gccgacggca ggtaccgggg
    10681 aacagacagg gaaatacaca atgcggacgg attcaccaat tatacggtct tttcactgtg
    10741 ggatacctat cgtgccgcac acccgctgtt caccctcacc gctccggata tggtgcctga
    10801 ttttgtcaac accatgctca atatcaggaa gcagcaggga aagctgcctg tctggtcact
    10861 cgccggaaat gagaccgact gcatgatcgg gtatcatgca gtgcctgtca ttgccgatgc
    10921 cgtgcacaag aaatttcagg gatttgacta cccggaggca cttgaggctg cattagagtc
    10981 agcatccctg gatttcaggg gtcttaatca ctaccgtaac ttcgggtata ttccttctga
    11041 acttgagaat gagtcggttg caaagacaat ggaatatgcc atcgatgact ggtgcatctc
    11101 acagatggcc tccgacctcg ggaaacctga actggcggct gagtatctga ggcggtcgca
    11161 caactaccgg aacctctttg acacaaccat cttttttgta aggggccgga tgagtaatgg
    11221 cagctggagg cccgactacg atccgctcta ttcgcatcac acccggtccg acttcaccga
    11281 gggaaacggg tggcagtaca catggcttgc gcctcatgat gttgacggcc ttgtatcgct
    11341 tttcgggagc agggagatgt tcctccggcg gcttgattca ctcttcatgg tgaccgagga
    11401 ggttaaggga gagaattcat cgcctgacat cagcggactc atcgggcagt atgcccacgg
    11461 caatgagcct tcgcaccaca ttgcttacct tttctccatg gccggcgcac cggagaagac
    11521 agagtcaatg gtgcggcgca tcatggcgga gatgtacact gacaaaaagg acggcatatg
    11581 cgggaacgag gactgcggac agatgtcagc atggtatgtc ttctcagcca tgggattcta
    11641 tcctgccaat cccgctgacg gaaagttcat cctcggcagt ccggttcttg acaaggccgc
    11701 catttcactt cccgggggaa agacattcac tgtcgtctcc gaaggcaatt caccaggaac
    11761 ggtaaatgtt tcggcctgtt tcctgaacgg tgagaagctt gaccgtaact acatcacctg
    11821 gaaggagata acagcaggtg gtgaacttag gttcgtaatg aaatgaacag tcctgcatca
    11881 cattgttgat aattttcagg ggcgattaac ccttccaatc attacctttg taccattctg
    11941 gtgctgccgg taggtcccgg cagtgaaaag ggaaccctgt cagaatcagg gacagtaccc
    12001 gctgctgtga agcccgttcc gcctgtcggc ggatcattac agccggaatc cttgccactg
    12061 tccgccattt ggcggatggg aaggcccggt gagtagggat gagtcagaag acctgccaga
    12121 tgtttcctgt tgaaggagct ttcgggaaca aaagtgatgg tcaaaaggtc atctgactat
    12181 attaaccgtg cctctgtgcg ttgcctgatc ctgtcagcga ctgccctatt tctgtttatg
    12241 actgtagcag tcagggctgc agcacaggac agtttcattg aagacactgt gaacatatcc
    12301 gcagtcacgg tcacggcaag ggcttccgca cgcctgtcgc cctacacggt ggtgaagatg
    12361 gatgcagaac tgctgtcccg tcatgaggga ggtgaccttg cctctctgct gcaatcatca
    12421 agtaacctgt ttgtaaggcg gaacggtaac cagggactgg catctgtttc cgtcaggggc
    12481 atgtcaggga gccacactct cgtgacatgg aatgaccttc ccctgaattc accaggcaac
    12541 ggcttcagtg attttgcaat cattccgctc atggcggctt caaccgtgag aatcacatcc
    12601 ggcggttcag accttgatga catctcaggt tacataggcg gcaaggtgga gctcgccact
    12661 gaccctgagt tcgataccgg tatgacggct gatgtcagcc ttggtgcagg cagctacggg
    12721 gagttggttt cctccgcctc attcaccctg ggaggtccgc ggttctatgg caggcttggg
    12781 gcatggagcg ggaagggcac caatgatttc aggttcatta acaaagatgc tccggaaggg
    12841 accagacatg aaaggaggag caatgcctcc tttgcgtccg gcggtataat gggtgacctg
    12901 gcataccata agggaaactc ccgggtaagc actcatctgt ggtttaacga tgcggatcgt
    12961 gagctgccgg ggcctgtcac aacggtgcag caggattttg gagagagaca gaccgacaga
    13021 tccttcaggg gggtgatgaa atattcactg gacggagacc ggcttaccgc aggactgaca
    13081 gcaggcggtt cacatgactt caacaggtac tatcacatgg tatcatcaaa caatggtgac
    13141 aacagctctt cttctttaat gctgaagatg cggctgggct acagggtgac cgggaagacc
    13201 gggttggtac tgaacgcggg ggacacgtat gagagggctt cggcgctcaa ctttgaaagc
    13261 cgggaagaaa ggaatgcatt ctctgcatcc gttgctgtaa gatataaccc tcttccccgg
    13321 ctgaatctcc tgctccaggt aagacaggtt gcagtcacag gggttaaggt ggccccggaa
    13381 ttcactgcag gggcatcgtg gatgctctcg cgcaacggag agcatcttct gaagatgagt
    13441 ctcagccgta acacaaagct ggcatgtttc aatgatcttt actggatccc gggcggcaat
    13501 ccggaccttg aacctgagac atcggccggg ggagagctgt catggtcatt tgcagagctc
    13561 aggccttcag gcgaccgcaa tacgcttgac ctttccatct atgcctcaag ggttgaagat
    13621 cttatccagt ggatccccgg acagtcggga ttatggcatg cggtgaatct ccgcagtgtg
    13681 aacgtatcgg gcctcgaggc ccgggcaggc ggccagaggt cgcccggccg gtggaagctg
    13741 aacggatgga tgaactacgc cttcacccgc tcggtgattg ccggttcgga tatcgcaaat
    13801 gacaggtctg tcggaagcca gcttatctat gcgccgcttc atcatgcgaa tatcaacctc
    13861 actgcaggca gggagtggct cagaaccggc ctgtcggctg cctgggagag caggcgtttc
    13921 accacatctg acaacaccga gtggctgcct tcctcaataa tgaccgaagc tttcgcggga
    13981 gccatctttc ctgtcggccc tgtaaaactc caggctgacc tgagggtgaa caacctgttt
    14041 ggtattccgg ttgagagcgt ccgcaattac cccatgccat tgagaacatt taaaatcaga
    14101 ttaaccctga cctggtcagg caaaccaaat gaaaatgaaa acgtactgta agatcattac
    14161 cgctttgact ttactgcttg cttcagcctg tgaaccacag gagaagccgg cctcttaccc
    14221 tgagggagag ggattctttg tacttaatga aggaaactat ctggccggaa acggcacgat
    14281 ctcattttac agccttgaga ccggggagat ttacaatgat ctttttggta ccgtgaacgg
    14341 gagggcgctg ggtgacatac cctcattcat ggcggttgag ggcagcatgg gctacatagt
    14401 tgtcaacaac tccggaacaa tagaagcaat tgacatggca accatggaga gcctcggtac
    14461 tgtcaccggc cttgcctctc ctaggcagat ggtgataaac gaaatgaaag gatacatcag
    14521 cagcctgcag gacggaggga tcaaggttct ggaccttgac ggaatggcag tatctgatga
    14581 aatcgaggtt ggttcatcaa cagaggcgat ggtcatctcg gccggcaggc tctttgcggc
    14641 tcactggtca gcaggaagca ccatcaccgt cacggacctg gccaccggca atctgatcag
    14701 gaccataagc gtcggactcg agccggagag catggttctt gactgcaacg ggaagctgtg
    14761 ggtactctgc accgggggct ggcagggtga agagattcca aggattgtga gaattaatac
    14821 ctccactctt gaaaaggagg ctgaactgca attccgcact gttgatgaca atccctcatc
    14881 actcacctgc aatgcggcag gcgacaccct ctactacctc gatgaagggg tcaggagaat
    14941 gcaggtgact gcagatgcac tgccgtccgg ggtttttatc ccggccggcg acaggttgtt
    15001 ctataagctt attgcaggcc cgcggggcat ggtggcagtc actgatgcca ttgattacca
    15061 gcagaaggga gacctgctga tctacaacag caggggagaa ctaattgaca ccgaacaggc
    15121 gggcataatc cccggttata tgcattatat gaaggattag ctactttttg ctaattttag
    15181 agctttaacc tcaggcaatt gaagagacta attctcatag ccattgtaag cctctccgca
    15241 tcgcttctcg tgtgcgggca accggagttc ccctcaggca gtcctctctt tccggagatc
    15301 ggaaagtggc atgagatcag tgttgatccg cagcatgccg aggctatgcg cgcagtgaac
    15361 cagccaaagg atcagccatt ccggtttgcc attccggttg aagtcagcct caccccgttc
    15421 aactccggat tcattgccac gagaggatca gaaaccatat gggtactgcc tgtgtcctca
    15481 aagggagcac tttcgttaaa tgtcatcctt tcaccttata acctgccccg gggagcctat
    15541 atctatgttt atgatcacga caggcagata gtgcggggag cattcaccgg agagagtact
    15601 tccggcaccc tctccatgcc ggttatgcct gttccggggg aaaggctggt actggaatgc
    15661 cacttccccg gtacccgcat tcctgaaaac tcggtggggg tgacgcaggt ggctcatgat
    15721 ttcacaggct tcttcagtcc tgatggaata aaggatttct attacggaca ctcagacgac
    15781 tgcgagattg acctcaactg ttcaaccaat cccctctatc tgactgcttc ccgctcagtg
    15841 gttcgtctcc tggtggcagg gaccgagctc tgtaccggtg ttatggtaaa caatacagga
    15901 aacgtaagca gggcttacct gatcacggca aatcattgca ttgagaatca gagccatgca
    15961 acgaatacgg tgtttgtcct caattaccgg agtccctcct gcgccggacc ggacatgacc
    16021 aacacctaca cccttaccgg gtcggagctg agagccacca atcctgagat agatttcgcc
    16081 cttgtggaac tgaaccagtt cccgtcactg gtcgcaagac catatttcac cggctgggat
    16141 attacggcca caaccccgac caataccttt accctccatc accccgaggg ggatgtgatg
    16201 aagctttcaa ttgatgataa cgccccggtc acagcgtcat atcctgtgga gggatttgtc
    16261 gcggcaggat tctggcgtgt gctcaggtgg gatctgggga caaccgagag aggatcgtcg
    16321 ggagcgccgc tgttcgacca gaacggaagg ctaaggggaa cccttaccgg aggagcggct
    16381 acctgtgatg atcctgcaaa cgactattat gccaaactga tcagaatgtt caatataacg
    16441 acggtctcct ccggtcatct gaggccatgg cttgatccgg caggcacggg cgccacactg
    16501 gtcagcggcc gtgatcccta cggatataac ctctccaggt cagataccat cggaggtata
    16561 ccggtgaacg accccggcac aactgacgca tactcatccc ccggatgggg ccttgccacc
    16621 ggcaacaatt ctgatgtgct taccaggtat gcagagtata ttcctttctc aggaaccggt
    16681 gagatagcct ggctcaggtt gcaggtctcc gcttcatcat tcatcaatgt tgccgactcg
    16741 gttcgctttt atatatggaa cggaggcgct cttccgggca gcgtcatagc caccagaaaa
    16801 ctccggatca acgaggccgt gagtggctca gtactggaag tcgatttcgg aaggaccata
    16861 aaggtgacag gatcatttta tgccggctac actgtctatt acaaaaatcc gctgaaccag
    16921 ccgcagactc agtttgccgt ggcacggtcg gcaccatggg ccctgccgtc gcagaataca
    16981 gcttacttct atagcggttc agcatggaag ccgttcaccc agcatccttc gttcccgatg
    17041 gctgtttcgc tcgggatcaa ggctgtaatg gttgaaaaca ccgtacttaa ccagcttgaa
    17101 gaccctgaac aggagactac agacctgaag gtttatccga atcctttcgc ctcagaggtg
    17161 acattcatac ttaccgaaag gggagtgacc agcacaaccc ttacgatata tgacaatgtc
    17221 gggagagtag tatcgacagg tgagtacagg aatatcttcc cgggcgagct cacagtacca
    17281 ttaccggggc tggcgcccgg gatttaccac tacggtctga gggctgactc ggttttttac
    17341 tcaggcaccc tcgtaaaaac cggtacggag tgaccaggat tctcgtgtcg gttatcgcag
    17401 cggcactggt cctctcctcg tgccgcaatg cgggaaacaa tcaggcatcg ggatccggcg
    17461 ttgacaatcc tgttgcagga aggttgacca ttgaaaaaga aaagaattat ttcagggtta
    17521 ccgtcagcga accctggcag aattcacccg gaatacagca cgtctggtat ctcatcccgc
    17581 gtgactccgc agtgccctca gatataaggg agactcagac aatcaggata ccggtgagga
    17641 gaatgatatg catgtctaca actcacattg caatgatgcg ggcgcttggg gctgacagcc
    17701 tgattgcagg aatctccggc tcgtcgctga tctatgattc tgttgttctg gaaggtctgc
    17761 gcagaggcac aatccgtgat gtgggttatg aggggagcct gaacaaggag ctggtcattt
    17821 cgctggaacc tgatctgctg atggcctatg gcgttgctga tccctccacc gggaacactt
    17881 ccagactttc cgaaacaggg gtgaaggtct tctacaatgc tgattatctt gaggaacacc
    17941 ctctctccag gtgtgaatgg ataaggcttt tcggcctgct taccggcagg gagcagcggg
    18001 ctgacagtat tttcatccgt gtctcaggtg cgtaccggga gctggcagat aaggtgagga
    18061 acggcataac cagtcgtccg gatgtgcttc tgggtgcacc gtgggaggat gtatggtatg
    18121 tttcaccatc agattcctat atcggcaggc ttattgagga tgcaggaggt cattacatct
    18181 acagtgatat gaccggtccc aattcagtgc ctttctcagt agaatcagtt ttcaggaggg
    18241 ctgcggaggc tgaactgtgg atcaacccgg gggccgcaga gaatctggcc gggatagctg
    18301 ccgctgaccc ccgcctggcc tctctcccgc ttttcagcga agggaaggtg tggaacaacc
    18361 gcaggagagt cacggaagaa ggaggcaatg actactggga gagtgccgtg gtgagacctg
    18421 atctgcttct cagtgacctg gtatcgataa ttcaccctga actgatgccg ggatatgtgc
    18481 aattctacta caggaagctg gaatgaggga gggtagcaca aggattgcgg tttcgatggc
    18541 aggtctcttc ctgttgacgc ttatcctgtt catggcagat atgctgattg gcagtacgca
    18601 gatggctgct tcagatgttt taaaggctct tttcagcagc gggcccgccg gagatgagat
    18661 tattgtgatg cagtttcgtc ttccgaaggc tcttacagct gttcttgccg gcgctgccat
    18721 gtcagtcagc gggttgctta tgcagacgct gttcagaaat ccgcttgccg gtcctgacgt
    18781 gatgggtgta agctccggcg ccggcctggg cgtagccctc acgctgctga cgctcacccc
    18841 gcttttcacc ctggctgagg ggagcattct tgccgggtgg ggagtgatac ttgctgcctg
    18901 ggccggcgcc ggagccgtaa tgataattat catggcggta tcggcacgca tgcgcgatat
    18961 aatgacagta ctgatagtcg gcatgcttct ggccagcgcc atctcggcag tagtgagcct
    19021 gatgcagtac ttcagcaacg aagtgatgct tcgtacctat gtgatctgga cgatgggcaa
    19081 cctcggcaat atttcttatc cgcagatcag ggccctcgcc cttgccaccg ctgccggact
    19141 ggccatagcg tttaccatgg tgaaaccact caatgccata ctgatgggtg agacattctc
    19201 aaggacggtg ggtgtgagaa tgcagagaac acgcattatt ctgctggcgt cggcaagcat
    19261 acttgccggg agtgtgacag ccttctgcgg gccgctggcc tttatcgggg tagccgtgcc
    19321 acatatagcc aggctgctct tcggcacagc tgaccatcgc atactgatgc ctgccaccgc
    19381 actgacagga gcagtgattc tgctgttgag tgacatcatc tcggtgatgc cgggcggcgg
    19441 gcacgtgata ccggtaaata ccgtcacatc actgataggc atcccggtgg tattatggat
    19501 cattctgggc aaagcgggtc ttgggaggag gttctgatga gaagaggagg agtaatagcg
    19561 gaaacacgtg acctggagat aggttatatg cggaagaaaa ccatctcggt tatgaaggat
    19621 ctatcgctcc gggcacgccg cggcgaactg gtggcactca ttggccgcaa cggcacaggc
    19681 aagagcacac tgctacggac actggtaggg ctgcagcctg cccttgctgg cgaggtcatg
    19741 cttgacggca ggacacttca cgaaaccgga tcgtcacagc tgcccaggat tgtcagcttc
    19801 gtctccaccg agccggtggc tgtacggaat atacgggtcc gtgacgtcgt ggcccttgga
    19861 cgattcccgt atacaaactg gataggtacc cggacagaat ctgacaatat agctgtcatt
    19921 gccgcactgg aggctaccgg tattgctgcc ctgggggata ggagcataga cagcatctcc
    19981 gacggcgaaa aacagcgcac acttatagca cgttcacttg cacaggacac cgggctgctg
    20041 gtaatggatg agcctactgc cttccttgac ctcccctcga gatatggaat agtaagtctt
    20101 ctccgccgcc tgacgcggga aaaggggaaa tgtgtgatct attcgacaca tgaccttgat
    20161 acggcaataa atgaggccga caggatatgg ctgatgaccg gtgacggggt aaccgaggga
    20221 gctcccgaag acctgatgct cacgggcagc ctggccagag ctttcgaaag cccgctgctc
    20281 tcattcagcg tcaatgacgg gacattcagc tttatcaggg gcagacagaa caccattgcg
    20341 cttgagggaa ggggaaaggc ggcaaagctt acgacaaggg ctctcgccag atgcggctac
    20401 acaaccgatc ctgatgctcc gcgtatcgta aaaatatatg agagaggtca catgacagag
    20461 tgggtggtca gcgatggaag caatgaaaaa cgatacgggt cactctatga cctggtcacg
    20521 gatctgccgg gtaatgatca atgagcctgg tatttccggt aggtatcgat aaatgaaggg
    20581 atatcctcca ccgagagccg ctttgctatg atctttttgt cgcggtcgag gatatacacc
    20641 attggtgtgg attcaacatt gtaaagggag tcgaacctgc tcatccgctc aggatcccat
    20701 ccgttgatcc atgtcaggcc attgtcgact atatatctct cccatttttc tcggtcatga
    20761 tgagtacaga cggcaaatac ctcgattccc gaagctttgt tcatgtcata atattctttt
    20821 aacagcggtg ttgactcctt gcaatgcccg caatcaggtt cccagaaata caatatcgtg
    20881 aaatcagcct tgacgtcgta caatgagaca taatgaccgg caaatgatcc catgaggagc
    20941 tccggggcct tcatgccgat caggttgggc ctcagccgtt caattctctt tctgaggtct
    21001 gaaacatact cttctgcagc ccatggcgct tttcctgcaa gatataccct gtcagccagg
    21061 tgcaccacaa cggcatcatg acccatgatc tcacttgagg catacctgtt catcagccat
    21121 acagcaacat actggaacat ggcactgtca acagcactct tctcaagaag cctgtcggct
    21181 tccttattga tcgagtcagg catctgtacc accacctgcc tgaagaactg gtcaagtttt
    21241 ccgccaagaa cgggacttcg gatcaggccg ggctcggaaa aatcaatgtg gtcaaagaag
    21301 tgatccttgt aataaagata agagataagg cgcgtcacag agtcgggatt attcgtgccg
    21361 ggaggcactg ttgctgcagg agtctcgaca ggtatcactg accatgctat cgcaccgaga
    21421 agcgttccct tgttggcttc ggcagtctcc ttcaggtatt gcttcatctt cttctcctgg
    21481 tccgtgagct cttttcttgc ggctgatgcc tccgggcttc cggcctgtag cccgccaagc
    21541 ctgccgttta ttttcattgc ctcctcctga agtgatttcc atttcctctg gtacgccaca
    21601 aaactggtgt tctcctcgga accactgaag gagatggtgg caaccggatc gctcttgctg
    21661 aaccggatgt caaaaaactg gtcctcgtca atcaggaact cgaaaaagag attgtcaggc
    21721 aggacaatca tatagacccc tgcaggcagt ctctcacttc ccatgaacct tcccctgccg
    21781 ccggagtccg ttacaaggct gtcgcgtaca tactgctggt tgccgacgtg atatgcaagc
    21841 cttagcttga ctgagggagc gtcctgtgcc gttactgtta aggagtggcc ggtctggctg
    21901 ctggcagcgg ttgtcagtgc tgcaaaagcc aggggcagta taaggttcgg gagtctgaat
    21961 ttcatctgaa aatgattatc accgtattca atatacaatt atcctgccat ttcgtgtggc
    22021 ggtggcagtc atcacatcaa cggggagacc tttggttttc ttgtgtttct cagtcacagg
    22081 gcagctgttt tcagttttcc gctgttccgc tctgcagcat cgtaagtttc agtgatccat
    22141 gctgatcctt agaatctcgg cgtatccgcc ggcggtgatg acagccctga gcacaaagtt
    22201 gaaacggttc aggtcagtgc agaggaagaa gtcggtagga gggctgaagt caagattagc
    22261 cgggttgaag aacctgcttc ccgatccctt cctcagtgcc tctgtttcct ttgatatgtc
    22321 gctttcgcga ccttcgagca cgtcttttat gtacctcgcg cagagcatgt cttcctctgc
    22381 cgattcaagg gccctgtagc ccatggcaac gagggtaacc ttctcgggcc tgatcattga
    22441 gatgtatgag gcgaccgccc ctgcgtttac cagtccggcc ccgaggattt ccgttgctcc
    22501 ggcggcgttg attatgcctt tggttcccgc cgtggtacag tgtatcatcg tcttgccggc
    22561 aactgagcct gtcagcatct cggtcggact gtttccgtag tcgaatccgg caggtttctt
    22621 ttcatcctgt tcacctgcaa taattacctc agcctttagt ttatccctta gtgcaaatgc
    22681 ctcctcaggc gtggcaacgt cgagaagacg gtttatgccg ctgtcaaagg catagcatgc
    22741 ggtgctgaag gcccggaaga catcaatgat aactgtcaga ccctgagcct cctgcgctcc
    22801 tttaataaat tcctttatgg tgatttccat gtcgggtttt tcaatttgtg aagataataa
    22861 tagtgtttga agatcatttc ttaaatttgc attccgttaa taatcagaaa atctggtgag
    22921 acctgttggt gaaataagtc agtcgattgt cagccgtgcc gtacagggcc ggaaggcagt
    22981 tgccgagagg ctgaagaaat cagtcccctt ctggattcgc caaaggcgta ttatatactt
    23041 gctttacaag ggaataaaga gggaaaatat ttatatcaag gcctctgcgc tgacattttt
    23101 cacgatactg tcagtgatac ccatggttgc actggcattc ggcattgcca agggtttcgg
    23161 gctgtctgac gaactgaggg cacagattat aatgcagttt cataaccagg aacaggttat
    23221 gaactggatt cttgattttg ctaacaagac acttgagcag acaagcggag gctggctggc
    23281 agggatagga gtagcctttc tcttttcaac tgtggggcag ctgctgagat atgttgagaa
    23341 caccttcaat tcgatctgga aagttgacga aacccgggtt tggtaccggc aggtgacaga
    23401 ttatcttgca gttatcattc ttgtgccggc tctcttcgta gcttcaagca gcgccacggt
    23461 gcttgccacc acgcgcctga atgacatgct tagccagtca gacatgcttg aagggctcaa
    23521 acccgttgtc tccttcctgg ttcagttgat accgttcata ctgctctgca ctctttccac
    23581 tgctgcattt cttgccatgc ccaataccag ggtcaagttc cgttcggcgc tggttgccgg
    23641 gcttgccgca ggtattgcat tgcagatttt gcagatagtc tatgttgaaa cgcagatggg
    23701 cctcaccagg cttggcactc tctacggaag cttcgctgcc attccgctgc tgatggtctg
    23761 ggtacagatg agctgggtag tgcttctgat gggagcccag ctctcatatt acctccagaa
    23821 tatcacccgc tacgaatttg agtttgatgt tcagacggtt tcacctcgtc agaaaaagag
    23881 gttgtcactg ctggtgatgc attcactgat cgttgatttt gtgaaagggg ccaaacccag
    23941 ggcgccggag cagatctcca tggagctgag cctgccggta cgcactgtcc atgaatgcct
    24001 tgacctgctt catgaggctt ccatggtcac cgaagtatgg aatgaagagc aggaaaaata
    24061 tgtataccag ccggccactg acattaataa aatgactctc tcgttcgtgc ttgaaaagct
    24121 cgagtcatcg ggctcactgc acaaaatagt catcaacaat tccgattacc ggaagataga
    24181 caccgccctg accaggttcg agagcctggt ggcctcatcg gaggtaaata tccttctcag
    24241 gaacctgtaa acctatttgt ttctgaacgg tgagagccgg aatcgtctca cataccttat
    24301 cagttcctcg agtgaattcc agttatcgaa gagatagtgt ggtgcttcaa ccattatctg
    24361 ccttgtctca gggtgcatcg accagtagct tgtaaggttt gaagccctga agacacggtc
    24421 ttccgaaccg ataagcgaat aatcccatac aaacccggtt tccaccggtt ccatcatccg
    24481 gttgtagagc cacctgagct catcgtggag tgatttttca gtgcgtgtag gtatgtaggc
    24541 gcggtttagt tcaaattggc tccttgtccc gaagagcctg aggtagaact tacccatgct
    24601 gtagttcgtg atattttcaa gggtggcttc aaaaatcctg agaggtatgc catacttgtc
    24661 atctgcggct acgggtgttc cattgatggc tatggtaaga tcaggtttta tcccaagctt
    24721 cctgctgtag tactcagcgg cccatacgcc cagtgaccag ccgatgacag taactcgccg
    24781 gtaggtcttc atctccggca gtatcagtgg ctcatcggcc gaatagttgt aaaaaagaat
    24841 aaagtcgtgg ttgtcggtgc agaggttgga gaatgctttt tcatcaattc cccaggtacc
    24901 gaagagaata acaagatctc tgttgttctc aaatctacgg aggaatgttt tcatcgggta
    24961 gaggaaaaga aaaaagagga gagggttacc gtggtacttt aaggaacggg cttatgagat
    25021 aatcccggaa tctactgtct gataaaagga agtaacctat ggtagtcagc ctataggtcc
    25081 atgtgaaagt caatgtctgc aatacaatcc tattgattcc cacaagtaac ccttactctc
    25141 ctcttttatt tcatcacttc cacaaatgta atacatatac ctcaactttg caaacaattt
    25201 cagaaaaaaa ttcaggtagt ttgtaaccgg ttgctaatta gaacaggatt gtgtaatttt
    25261 agactgaata tgatgccaat tattcctctg acattaaatc cattagtatg atacctgaaa
    25321 taatcagcat ccggcatgac gacggctcag gattcagggc cgcatggatg gataacgaga
    25381 gcggctttct ccacctgcat cccgaatatg aaattgttct gaatatttct ggcaacggga
    25441 cccgtgttgc aggtgattcg gtggagctct tcgaccgtta tgacctggtg atgtacggtc
    25501 ctgacctcag gcatttctgg aacttttaca ggaaggatga aaatgaaacg ggcaggcatg
    25561 caataatggt tcacttcagg cgtgattcgc ttggcgattc aatgctttcg caattcgaga
    25621 tggctgacct gaggcggttg ctggaaaatg ccggccgggg gattgccttt ccgcccgaga
    25681 cgggcgaagc tgcagaggag cccatgcggc gcctgctgtc cgataccggg atgaaaaaga
    25741 tggtcagctt cttcaggctg ctcgaaatac tctgtgcggc accgagattc aagattctgt
    25801 caggcgagaa ccgcgaaggg gtgcatgaga ttactgacag tcgcctgact gaagtatgtt
    25861 cttttgtcag ggataatttt taccgcccga ttcctgttaa ggaggtcgcc gtggtggcaa
    25921 agatgagccc ccattcattc agccgcttct tttcacgcag taccggaagc ggatttgtcg
    25981 actatgtgaa ccaggttcgt accaacaagg cctgttacct gctccgggaa accgaccacc
    26041 agataaacga aattgtattt gaatgcggat ttgcaaccgt ttccaacttc aataagcagt
    26101 tccgcaagca tgccgggatg tcagcacgtg actaccgggc gcaataccgg aactgacatc
    26161 ctgcctttct gcgggaaaat cactttcaac tgatgatatt attcatgccg gagtttgatc
    26221 cctattgcta aatttgcctc tgaaccaact aaaaacatct cgatcatggg aatgaccata
    26281 accgaaaaaa tcattgccgc tcactccaaa tacaaaagcg taaaaccggg tgacattgtt
    26341 gacattacaa ttgacgcccg tgcagccagg gacttcggcg gcgccaatgt cgtcaagaac
    26401 ctgaacgatt ttggactgaa ggttgatgac cctgaaaaga ctctcttcac gttcgactgc
    26461 aatcctacag gttcagacca gaaatatgcc gttaaccagc acatatgcag gcagtatgcc
    26521 cgtggtaacg gaataaaggt atatgacatt gatgcaggca taggcacaca ccttctcatc
    26581 gaggagggct atgtatggcc gggagccaca gcggtatcaa ccgattcaca tgcaaacatc
    26641 ctgggtgctg tgggtgcatt cggtcaggga atgggtgaca tggacattgc tgctgcatgg
    26701 aacagcggga gggtatggtt caaggtcccg gcatcggtga agctcacact gaagggcagg
    26761 atgcctgagg gtgtggctgc caaggatgtc attctgaacc tgctgcgcat gtatggcgcc
    26821 aacactctgc tgggttacag cattgagcta tatggtgatg cagtggaacg gatgtcgctt
    26881 gatgaacgga tcaccatagc ctccatggca acagagatgg gagcaatcat aattctcttc
    26941 ccgccaacgc cggcaattat cgggtattgc cgtgctcgcg ctcgcgcacc gtttgaacct
    27001 gtccacgctg acgcggatgc cacctacgca ataacagcag atattgacgt cagcaccttc
    27061 gtaccgatgg tggctcggcc tggcgaacca catgacacgg taggggtcag ccagctcccc
    27121 gtcacaagga tagactctgc ctttatcggc agctgtacca acggccgtat cgaagacatg
    27181 agagcgacgg ctgagatact gaagggcaga aaagtggcac cgggcgtggt actcaagatt
    27241 gtgccatcaa ctgatgccgt atggaaacag tgtcttgatg aaggacttat ggatatcttc
    27301 aaggattcag gggcgctggt ctcaaatgcc ggttgcgctg gatgtgcagc cggacaggta
    27361 ggtcagaacg gcaagggtga ggtcaccgtc agcacaggga accggaattt ccctggcaag
    27421 cagggacagg gaagcgtatt cctcgcgtct ccggccgtag tggcagcatc agcgattgca
    27481 ggatatataa caactcctga aatgattccg gttaccccgc ctgaattcaa attcagcgca
    27541 acacagcctt cggccgctgc ccctgttaag agcacgcaaa ctgacagacc caccgttatc
    27601 cgcggaaagg tatggctgat agaacgtgac aacatcgaca ctgacatgat cttccacaac
    27661 cggtacctgg ccatcactga catacgcgag atgggccagt atgcatttga caacctggaa
    27721 ggttacaggg attttgcaaa gaaggcgcag gcaggcgata tcattattgc cgggaaaaac
    27781 tttggaagcg gcagctcacg ccagcaggcg gttgactgct ttgtctcact gggcatacag
    27841 gccgtcgttg cgcaatcgtt cggcgccatc tatgagcgca atgccattaa cgctgcattc
    27901 cccgtactta catactccag ctttgaaaag atagacctcc gcgaccagga tgtcatatca
    27961 gtcaatattc tgaccggcga ggcagtgaat gaacgcaacg gattatcgac tttaattaac
    28021 ccattctatg atgctcagta tgagatttat aaaaagggtg gtttattagg taaaatttaa
    28081 cgtaagacat atcatcatat gaaaccaagg acatttgttg aaaaggtact gggcgctcct
    28141 gagggggcgg tagtattcag gaaacccgat ctgatactga cgcatgacaa cacctccagc
    28201 atcttccaga cattcagaaa aatgggaggg gagaaggtgt cagatcctga ccagatggtc
    28261 atcgttctcg atcacaatgc cccgcctgct gatgccaaac tggctacgca ataccaggat
    28321 ataaggaaca tagtggcagg acagggaatc aggaagtttt acgactcagg gatgggtatc
    28381 tgccatcaga tgatgtcaaa tcatgcccgt ccgggaatga ttattgtcgg ttctgacagc
    28441 cacacctgta cggcaggagc cttcaatgcc cttgcggctg gaatagaccg caccgaggca
    28501 gcaggaatct ggaagagagg cgaaacctgg tttctcgttc ccccttcaat gaggattgat
    28561 ctgaccggcc ggttacgtcc gggtgtcagc gcaaaggacc tgtcgctgtg gatcattggc
    28621 atgctgggct ctgacggcgc caattacatg tctattgaat ttcatggaga cggggttgct
    28681 tcgctcagcg tgagcgagag aatgactgtt gcaaatcttg catctgagat gggggccaag
    28741 aatgccgtct tcccggatga tgaggtgttg cagaagttca tcggttcaaa cagtcccggc
    28801 atctgggctg atgagggtgc ggaatacaaa agccgcatat ccattgacct gtcgaaggta
    28861 tttccgctgg tggcagctcc tcatcatgtt gacaatataa aatcggttgc ttcggtggcc
    28921 ggcaccgttg tgcatgaagg gctgataggc acctgcacca acggcaggat tgaggatctc
    28981 cggacagcag catctgtgct caggggtgct caggtgaagc cgggcttcca gcttcatatc
    29041 atccctgcat cgaaggagat ttatcttcag gccatcaggg agggtatagt cactgacctg
    29101 atagaggccg gcgccaatat tctcagtccc agctgcggtc cgtgcctggg cacaggccag
    29161 gggatacctg ccagcggtca tacggtcatc tctactgcca acaggaactt cctgggccgg
    29221 atgggtaaca aggaggcttc catttacctt gcttcgcctg ccactgtggc tgcatcggct
    29281 gtcaccgggg ttattaccga ccccagggtt acaaggggtg acgaggtatt tccgttcaat
    29341 cctccccaga gccagaccaa caccatcagg gagggagaga acaggaagga aggcaatgta
    29401 tggaattatg cagatgttga caacctgaac actgaccaga tgtttgccgg caagcacact
    29461 tactcagtca tgagttctga tgcggctgca attctgccat tgctcttcgg cgactttgat
    29521 ccctcgttca gtgcaaatgc aaaagccggt gacactatca ttgccggtga taacttcggg
    29581 tgtggcagct ccagggaaca cccgtcagtg gggctcgccc atctgggaat cagggctgtc
    29641 ataatcaagt cagtcaaccg tatcttctac cgctcctcta taaaccaggg gctgccgctg
    29701 atagtgcacc gggaggctgt ggaggcgtac cgggcaggtg atgcggtgga gatagacttt
    29761 gacaggggta tgctgaaggt aggagagaaa cagttcagat ttgaaccact gcctccggag
    29821 cttaaggcaa tcatagagaa gaagggcctt gtcaactgga tgaagacggc ctgacctccg
    29881 tgcagggtga agttccctgt tgccaaacgg aaaaatctgc ttatatttgc cgcggttttt
    29941 tataaaccgc ggtacggttc cgtagctcag ttggatagag caacagcctt ctaagctgtg
    30001 ggtcgagcgt tcgagtcgct ccggaatcac gaaagcctgg gctatgacgc ccgggcatga
    30061 cagaggaagg tggcatgaac accatcagtt tcagatccgt acttaagatt tccggactca
    30121 tcctgctgat atcagcatca tcgttcctgc tttgcattcc ggtggcgctg atttattctg
    30181 aacctgtcag tccgtttctc cttgcatttg tgacggcatt aactcctggt gcgctcctgt
    30241 acttcctgat aaacagtccg ctgcatcaga aggtaacatc acgcgagggt tatctcagcg
    30301 ttacgctcgg atggattatg ctggtcattt ccggcacctt tccgtatatt ttcagtcatg
    30361 agttgaaggg atttgttaac atgctcttcg agagcgtgtc tggttacact accaccgggg
    30421 catcaattct taccgaggtg gagagtcttt caaaatcagt cctcttctgg cgcagcctga
    30481 cacactggat tggcggtatt ggaataatac tgcttgttat tattgtgctt ccctcactca
    30541 aggtcggagg atacagcctc ttctcgctcg agtcatcgct taaggaaaag atactgccca
    30601 ggaccaaatc aatagccatt accgtattga tgatatatct tctgcttaca gcactcctgg
    30661 taatccttct tactgccggc ggaatgaatc ttttcgacag catctgccac tcattcggca
    30721 cggttgcgac aggagggttt tcaacaaaga acacgagcat cgcagggtac tctggttaca
    30781 tccagtatgt actggggata ttcatgttcc tttctgctgc cagtttcgta gtcttctact
    30841 tcattctgaa acggaacttt tccagggtga aggccaatga ggagctgtgg ttctatctat
    30901 tcttcaccac ggcatcagtt gtggctgtca cgctggttct ctacacaggt actgaaagaa
    30961 attttgaggt ggccttcagg catgcatttt tccaggtaac ttcaaccatc tcaaccacag
    31021 gtttcgctac gactgattat aacgtatggc ctcaggctgc tcttgtcatg atctttctcc
    31081 tgatgtttgc aggcgggtcg accggttcaa ccaccggggg cataaaaatg gcccgtcatc
    31141 tgatcgccct gaagaatctt cgcaatgtga ccgtgaggct tctgcacccc tctgccgtga
    31201 tacctgtcaa gctcaacggc caggtggtgc ctgaaagcat caacagcctg atgactgtat
    31261 ttatccagct ttacgtgatc attttcattg cagggacact gattattctt gtgtcaggca
    31321 tacctgcaat tgaggcggca ggtgcctcgg tatcagccct ttcgtgtgtc ggaccgggat
    31381 tcggggcgtc gggaaatatg ggaaattatg cccacttcaa cgccgttgcc aagctgacca
    31441 tggtggtact gatgattata ggccgtcttg agatatttac gatccttgcg ctcttcaccc
    31501 gtacattctg gaagaaataa atgttgtttc aggcatatca tttctgttct ttttctctta
    31561 aaaagtgtta taatttcaaa aactcgctta cctttgcacc caattaaaaa tcatctcatg
    31621 aataaaggaa ttgttaaatt tttcaatgtg tccaaaggtt ttggattcat taaggacgcc
    31681 aactcatcca aagagtattt tgttcattct accggcctga tcgacaagat caaggagaat
    31741 gacgaggtaa catttgacct ccaggagggc aaaaaagggt taaatgccgt taatgtaaga
    31801 cttgcgtagt taacactata atgtacattt aaataccctg gaggctgcct caatgaaaat
    31861 tgaggcagcc tctgtttctt atagatgtgt cctccggaac agggggactt ttttttgttc
    31921 agggaaacaa ggtatatgat ttacttgtta accatgtttg gtttgcacag gacactaata
    31981 cgcggcgaag gaacaggcag gaagtaaatg attttttcct gctatttggc cacgtcaaat
    32041 tcaatcacat acccgtaccc cctccagatg gtgctgagag cgatgttttt cattccggcc
    32101 ggcacaaggt ccacgggggg taccaccttt tctgccgagc cggtgaaaat attgaacagt
    32161 cttcctgcct tctccgtttc atcggcaatc acaatatggg ttcccggctt tgcaaccctg
    32221 atcatctcgc ggatagccag acccttgtct tcaaagaggt taatggcacc taggtgaaat
    32281 accacatcaa atacattgtc cctgtatggc agtttctgcg catctgcacg gaccagctca
    32341 gaattaatct tccaccggtt caggttactg acacagtgtg acatcatctc ttcctggatg
    32401 tcaaggccat aaagctgaag tcctgctgac cgcctggcaa gccacaataa attctccccg
    32461 taaccaatac ccgtttcaag cacggtatct ccatcattca cccgcagtct gcccatgact
    32521 tcattcctcg cacttttcgg gcctccgcac agaaggaaca tcatgttggt aagaggcgta
    32581 tagaccttcg caagcaccga ccggaaaaac ttttccctcc ggtttttaaa gaccagtccc
    32641 tcgccaccca gaaaatcgta atagccaccg aagtgcctgt agctgacgtc acatttactg
    32701 caactcagaa gatcatgatt caatcggtca gccttactgt ctgcctgatg aatcagctca
    32761 cccttgcatg acgggcagca tagcatttca tttacagatc gtgccatacc ctgtttatta
    32821 ttttatgcac aatttaagat ttattgtgaa attattgaca aggctcttta aaaattctaa
    32881 atttgtatat gtttagatta tattatacaa tcaactgact aatgatcgta tgcaaatatt
    32941 aagacagcct tactgacagt ttcaataagc taatgtaatg aaagtgcttg tcaccggttc
    33001 ttcaggttat cttggctcaa ttgctgtgag tcatcttgtt gcaaaaggat gcgaggtcat
    33061 tggcctggac atgaaaaagc cggacggaaa ggtggacggt gacggtttca ggttttatga
    33121 atgcaattca accgacggca gttgtgtaag ggatattttc tcacgggaac tgcctgatgc
    33181 cgtactccac tttgcctgtt ccatgaacag gttacggaac aggaaagagg agttcggaat
    33241 ggatgtggga ggatcattca atgtgataga ggcagcccgc aggaccccct cggtcaggaa
    33301 gtttgtcttt tccagctcag ctgccatcta cggccctgca gcaaggagtg agctgtggct
    33361 tgacgaggct gagccactaa accccggcca ctatacttac ggcctgaaca agaggctgat
    33421 tgaacagatg cttttccggg taaacggcga ccatccgatg cgaaccgtct cactgagaat
    33481 ctgcacggtg gtagggccgc gctattcaag gcccaagtca gtggtttcca ttctgttaag
    33541 actgcctttc cttccagaat ccttcagcag gacctctgtt cagttccttc atgaggatga
    33601 tttcaacgag cttttaaacc tcgttctgca tgacagcgag attgaaggtg tttttaacct
    33661 ggcacctgac agttgttcgg ttgttggtga aattgttccg cccgcaaggt ttcaccggtt
    33721 cccctgttca gccatcaaac cggttatgtg ggcgctatgg aatctcagga tggtgaacct
    33781 tcagcctgcc ggcctgggat tctgtctgta tccggtggtg cttgactcaa agcgactggc
    33841 acgaaggtat gattacaggt tcaggcactc atcaactgag tcgttcctgc tgacgaagga
    33901 gcgaaacagt ctccccccgg ttgccaggtt ctgattatca ctccctgttt tccgtgagtc
    33961 cgaaacggct tccggccaca tgacttatca gcctgacagt ttcgtcaatc ccgagcacgg
    34021 aagaattgat gcagagatga taagattctg cggcgcccca tgtcttcccg ctgaagtaat
    34081 tgtagtacga tgagcggccc ttgtcagtgc gctctatcag ctcctttgct ttggtgtctg
    34141 acagcttgtg gctgattgcg atacgtttaa tgcggtctgc catgtcagcg ctgataaaca
    34201 ggttcaggca gttccgttcg tccttcatca cataatctgc acacctccca acaaagatgc
    34261 atgatccctc accggccagc gttctcatca cgtcactctg tatcctgaac agggattcgt
    34321 tgctgagata gtaattcgag aagaaatcat cggtcagcga ggtacgcagt cccagtattc
    34381 ccggaaagag gctgaagtgc ttctgctcat caaccctctc gaagaactcc tccttgagcc
    34441 cgctctgctg cgaggcgata cggatcagct ccttgtcata aaacgcaatc cccagcctgg
    34501 cagagagttt ctgtcctatc tcccttccgc cgctgccaag ctgtcttcct atcgtgataa
    34561 tataacggtt taccattggc cggtggattt tatcataccc aaatttaatc tttaaaatta
    34621 atttccggct atgtgcatgc atatattgcg gataaggtac agccggatga aagtgacggc
    34681 attaacccaa aaaatagtac tttagttgcc gtattttccc ggacaggagc atgcaccatg
    34741 aagaagaaat acattctctc ccttgatcag ggaacgacaa gctcgagggc catccttttc
    34801 ggaagcgacg ggcagcctgt agcctcgtcc ctcaaggaat tcacccagat ctttccacgg
    34861 cccggctggg tggagcatga tccagctgag atatggtcca ctcaggccgg tgtggcggcg
    34921 gaagtggttg ccggaagcgg tgctgactgc tctgacattg ctgcaatcgg catcacgaac
    34981 cagcgcgaga caacagtggt atggaacagg agaaacggca agcctgttta caatgccatc
    35041 gtctggcagg accgtcgcac agcggatctg tgtgacaaac tcagggcgga gggccatgga
    35101 gcaatgatag ctgaaaagac aggcctggtg attgacgcct acttctcggc caccaaggta
    35161 aaatggatac ttgaccatgt cgatggtgcc agggcagcgg ctgaaagggg tgaccttgca
    35221 ttcgggacaa tagactcatg gctggtctgg aacctgacag gcggcaggct gcatgtcacg
    35281 gatgtttcaa atgcatcacg caccatgctg ttcaatatcc gcagtctgca atgggatgat
    35341 gtgcttcttt ccctctttga aataccttca tcaatgctcc cggaggtgcg ttcatcatca
    35401 gaggtttacg gttattctac cggccttttt gcctcaggga tccccgtcgc agggatagct
    35461 ggagaccagc aggctgctct tctgggacag atgtgcgttg atgaaggaat ggtcaagaac
    35521 acctatggta ccggttgttt tatgatgatg aataccggca gtgaaccgat gagatccggc
    35581 aacaggctgc ttgctacagt ggcatggaag ataggagatg acacaaccta tgccctcgag
    35641 ggcagtattt ttataggcgg ggcagtggtc cagtggctgc gcgacgggct cggcatcata
    35701 aggcagtcat ctgaagtgga gcgtcttgca tcacaggtaa gctcagcgga gggtgtctgt
    35761 tttgtgccgg cattcgccgg cctgggagcg ccatactgga accagcatgc caggggcact
    35821 attaccggcc tgacacgggg tacaaccgca gcccacatcg cccgtgccgc ccttgaaagc
    35881 atagcatttc agacacttga tgtgttgctt gcgatggaga aggattcgaa tttgaaaata
    35941 aaagagttaa gagtcgacgg cggagcaacg gtcaatgaca gcctgatgca attccaggct
    36001 gatatccttc agacagacgt tcttcgttca ggggtaacgg aaacgacagc attgggggca
    36061 gcctttcttg caggtctggc gacaggattc tggggcggga tggatgagct gcggggtctc
    36121 tggcaggccg gacgtcattt tgcccctgca atgaaaccgg aggaaagaaa gacactcata
    36181 caggaatgga accgtgctgt gatgactgcc ggtgcctggg cagatgatgc cggaagataa
    36241 cgacctgcaa caattaacct gaacgaacct gcataaaaat ataacataat gaaaaagaca
    36301 ttcctcctgg ccgccttgat gatgctgatc atttcaccgg gtcttaaagc ccagaatgct
    36361 gacagtgcag atgtttatat gatcacctgc gcacccggta cagctgccta ctcgatctac
    36421 ggtcatacag cactcaggat aaccatgcgc ggcacagaat tcgacatggt ctataactgg
    36481 ggcatatttg acttttcaac acctaacttc gtatacaggt ttgccaaggg gaggctcgat
    36541 tataagctgg gagcttacag ctatgcaagg ttcctcgaag agtatatttc cgaggggaga
    36601 tcggtatggt cccagaagct aaacctgacc acagcggaga aggagaggct gtttatactg
    36661 atcaatgaga acctcaggcc tgagaacatc aagtacaggt atgacttctt ctttgacaac
    36721 tgcgccaccc gggtacgcga tatcgtcgcc gcctctgcag ctgacaccgt cattttcccg
    36781 gtaaaggaga agcgcctgag cttccgccag cttatcgacc cgtaccagaa ggtacttccg
    36841 tggctggatt tcggcgccga tatgctcctc ggactgcagg ctgacaggaa ggcatcagtg
    36901 gctgatgaga tgttcctgcc cgtatacctg agagacaact tcgccggaag cctaatagtt
    36961 catgacggaa gacaggagcc gctggcaggg ccgacggaga aggtagtgga cagacctgta
    37021 acacagaccg ggaagaaggc atgggtgcca caggctgtat tctggctcat attcgttttt
    37081 gtagtgcttg tgacatttgt ttttaaaaga cccggactgg aaaaggttct ggattacata
    37141 ctctatttca ttttcgcctt aattgcactg ttgctgattt tcacgaatat attctcggaa
    37201 catgatgcac ttcatctcaa cctgctcttc ctgggaataa acgtactgat aacagtactt
    37261 ttcatcatga tgctcgcggg gaaaaaatca gtgaaactga gcaggttaac gttaaccatt
    37321 tcggtccttt ggtttcctgt cgctcttatt gccggccagg gaattaaccc tgcactcata
    37381 ccactggtcc tgacgataat ggttcgactc ttcaggcact gcggtttcgg gaaatcgctt
    37441 taacacatct ttaacaacat ttaacaaggt tttttaatat cagaaatgat ctttgcgaag
    37501 cttttaataa agggcaaaat acaatttaac aacatatgga acatacaacc gacatcaggc
    37561 ttctgaatga aaagattgaa aaggaaagcg cctttgttga tcttgtcagg caggagataa
    37621 acaaggtgat agtggggcag cgtcatctga cagattctct gctcatcggg ctgctttcga
    37681 acggacacat tcttcttgag ggtgttcccg gactggcaaa gactctggcc ataaagaccc
    37741 tcgcgtcgat aattgataca aagttcagca ggattcagtt cactcctgac ctgttaccgg
    37801 ctgacctaat cggaacaatg atctacagcc agaggaaaga ggagttcgtg gtgcgcaagg
    37861 gtccgatctt tgccaatttc atacttgccg acgagattaa ccgctccccg gctaaggtgc
    37921 agagcgccct cctggaggca atgcaggagc ggcaggtgac tattggtgac actacataca
    37981 ggcttgatga gccgttcatg gtgctggcga cgcagaaccc gattgaacag gagggcacct
    38041 atccgctgcc tgaggcacag gttgacaggt tcatgctgaa ggtggtgatc acctaccccg
    38101 agaaggagga ggaacggctt atcataaggg agaacctggc cagcgagttc cccgttgcaa
    38161 aaaccatact gaagccggaa gatataatcc gcgccagaag cgtggtcagg gaagtgtaca
    38221 tcgatgagaa gatcgaaaac tatatcctcg acattgtatt tgccacccgt cagccggaca
    38281 aattcggtct ttcgaagttc acaccgctta tagcctacgg agcctctccc agggcaggga
    38341 taaacctcgc actggctgca aaatcatatg ccttcataag gcgaagggga ttcgtggttc
    38401 ccgaagacgt ccgctctctc tgtgccgatg tcctccgcca ccgcataggt ctgacctatg
    38461 aggctgaggc tgaaaatatc actcaggatc agattattgc agaggtgctt aataccgtcg
    38521 aagtcccgta gaaggatgaa gagattacaa ctgtcactga tcctgctgac atttctgatc
    38581 cctgtaaagg ctcaggaatt ctgtggcatg actgaaaaga gcatccgtgc tgtgatggag
    38641 agggatttca agggtctgac gcctgacgac aatgtcagga atgatgtcta caggtacctt
    38701 aaataccatt cggttgacga caatgagaca tggctgatat tcctcgatga gagaaacagg
    38761 tgcaaagggg tcaggataac atacagtaac agtctttatg atacaaagat cagcgaactg
    38821 aaccggaagt acgggcatga cgatggagga aagtggagtt acaggctgga acggaaccgg
    38881 attgccgtca cggttcaccg tgatgaatgg ttttttactg tcacacatgt gcggatgtaa
    38941 catctgtaag ggaagtggaa acaacagaac tgttaaagaa ggtaaggaag attgagatca
    39001 ggtcgcgcgg gctgacccgc cagatctttg cagg
//
LOCUS       DAOPXU010000012        39023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_913_length_39023_cov_13.261522, whole genome shotgun sequence.
ACCESSION   DAOPXU010000012 DAOPXU010000000
VERSION     DAOPXU010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 39023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39023
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_913_length_39023_cov_13.261522"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..535)
                     /locus_tag="PKK03_02095"
     CDS             complement(110..535)
                     /locus_tag="PKK03_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83212.1"
                     /translation="MEENAASPTVVTVEENRMKKHVNIAGILQIVFGSFLVLGALTIA
                     VLFGFIDQFVDDPTAIKVLGIISTPLVVLMLLFGGGMIAGGIGLFYCKPWARILTLVL
                     GAMGLLNVPIGTLKGVYIIWVLVQSETISLFEKGCPGKV"
     gene            653..880
                     /locus_tag="PKK03_02100"
     CDS             653..880
                     /locus_tag="PKK03_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83213.1"
                     /translation="MKKLLYASACLIITCSFNSCEILGDGCQVCRTVSYENGNAIAWG
                     TEAEYCGQELISIKAIPPSTVSGVTTYWECR"
     gene            1060..1464
                     /locus_tag="PKK03_02105"
     CDS             1060..1464
                     /locus_tag="PKK03_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX83214.1"
                     /translation="MLNITGKRIAIVEDDAASLKYYETLLKETGADVFTYRNGKEFVE
                     AITTGREHFDLVFMDFLVPLVNGIECTRAFRQVNRSAPVILITAYYSEQTKNEAYIAG
                     CTEYNLKPIFPDKIHLILEKYLAPRPAYQMYD"
     gene            complement(1550..4756)
                     /locus_tag="PKK03_02110"
     CDS             complement(1550..4756)
                     /locus_tag="PKK03_02110"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNX83215.1"
                     /translation="MIKKYRLWDNILGWTVFVVASVTYLLTLEPTMSLWDCSEFIASA
                     FKLEVGHPPGAPFFMILGRFFANFAGGDESRVAMMINAMSGLASALTILFLFWTITHL
                     ARRIFLRQESDYTAGRIIAVLGAGLTGSLAYAFSDTFWFSAVEGEVYATSSLFTAVVF
                     WAMLRWEDVADEPHADRWIILIAFMMGLSIGVHLLNLLAIPAIVFIWYFRKYRFSWKG
                     FAVSIGVSILLLVLIMYGIIPGIVRVTSAFELFFVNSLGLPFNSGMYIHLLLFIAALV
                     LSVWYSFTHSDRMRIFLLAGTTLLLSGVWLLTGSLLLNLVFLAAIIYGVWLLSTKQKA
                     ALNTIMTSLLVIMIGYSSFAVILIRSTANPPMNENNPSNPFALLYYLNREQYGRRPLF
                     YGPYYNAPVTDYAKGKPTYNPSGDRYIITSRETETEYDERFMTVFPRMWSDSPDHVRE
                     YERYVGTKGKPVRINDPQTGEAKTLRVPTFGQNLGFMFRHQLGFMYFRYFMWNFSGRQ
                     NDVQSTGGPVNGNWITGLDFLDAPRTGSVKGMPEDMRNDPSRNKYYLLPLLLGLVGFL
                     WHFNRDVRNWWIVLLLFVMTGIAIVIYLNQYPNQPRERDYAYAASFYAFTVWVGLGVL
                     ALFELFRKAAGETPAAVIASVLTFAAVPALMGSQNWDDHDRSGRYLARDVAFNYLNSC
                     APNAIIFTGGDNDTFPLWYAQEVEGVRTDVRVCNLMLLNTDWYIDQMKYKAYTSEPLP
                     VSLQKELYYDGVNNQVAVLEKVREPATAKEVMQFISSGSELSKLKMYGEDMWYIPTRT
                     IRIPVDSAKVLANGTVKPEDADKIVPYIDITLKGSWIMKSQLLVLDILAHNDWDRPIY
                     FVTGQTDDALGLEEYFQLEGLAYRLVPVKGVNKSWFEYGRIDTDILYENMMNRFSWKG
                     ANDPDVYIDFYHNRTLLVVRARLNHARLAGALVAEGDTARAVQVIDRCLELFPVSNVD
                     YDYYFGDIISACFATGRKDKAKELAGEFADYYAERAGYLLDQRPSVAYYAGAEIATGL
                     QMMLQAIKVCYDNGEIALAEDINGKYNELYARYAALNQ"
     gene            4864..7779
                     /locus_tag="PKK03_02115"
     CDS             4864..7779
                     /locus_tag="PKK03_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83216.1"
                     /translation="MKRCIILLIMSLLLMARAGAQYYDTGQDPASLRWLQIRTPHFRV
                     IYPDDFGGEAFRYARLLEESFEKLSVLYPGVRTNIPVIIHSHSMQSNGYVSWAPRRME
                     LYPLPGQDNLPMYPAAQLAVHETAHVLQLGSLNTRGFGRALRFILGEQAVGLSAVQIP
                     AWAFEGDAVYAETATGLSGRGRSNAFIRGAMALTLRPEEIYGYDKMLSGSYRNFTPDH
                     YVFGYLMMNHLRTIDPDTWNRVTERVSSGYFPNPVNTVLTRETGLSKRKLYDSAFAAL
                     ERNWSKSSPEGVKEYTPLSLSRRRNYVSHFTPYRSDDGRIISLRTSLSDPSRFVITEN
                     GGGKELNITTTGYIYPYFFSFSGNTIVWAEQHPDIRWDNRDYSVIKRLDLPGGLITDV
                     TSRTRYTAPDLSPDGRTIVAVSTTPDMVCSLVFLDSHTGEVLMNLVPPESLILQRPAW
                     SSDGREVTMVTLNQQGEGIRTYRPTGKIWTVNLEESHTDIVQAKIHNDTLFFLAQGDG
                     SDNIYRIAGAGPVERVTGSRFGISGFSVTGSELLFSDYTAGGFVIASEKTSATAGPAF
                     VTGHEIFPPVAPMPGKVPGKDPSPPVVHTPAADSLSDTPMPLIPEPRPYRKISNLFNF
                     HSWFPFYADLDEIRTDPTTIRPGLTLMSQNHLSTLISTVGYEYSEGNHYLHSGVTWKG
                     WHPVIDAEAEWGGDQLIISDTSENQPPENPGTDLQLNLSIYDQLWFARGKFRQMLMPA
                     LYISFRNRDTWISAENRFDRDVLSLTGRFYFSNTFRTAYRDINPRWGQVFDLRLTTTP
                     WDTKLYNSKSYARTILFFPGALSNHSLSFRAGWENQAPARKQIYRNTLPWPRGYDYDL
                     VAEKLFSFSADYTMPLFYPDLAAGSFLYLKRIRGTLFYDYSKGVDVRNYDDRSFTAGA
                     DWFSSVGSELMADFYVLRFPFEISAGVRAGYMPREDKYFVNGAFSVNIYGTVLGRER"
     gene            complement(7738..9180)
                     /locus_tag="PKK03_02120"
     CDS             complement(7738..9180)
                     /locus_tag="PKK03_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HNX83217.1"
                     /translation="MDSNEFRRYGHEIVEWIARYFENVEQYPVKSRVRPGEIKNALPE
                     LPPSGSESFDLFMKDFDEVIMPGITHWQSPNFFAYFPANSSPPSVLAEMLTAALGAQC
                     MLWETSPAAAELEERMMEWLRSMTGIPPEFEGVIQDSASSATLAALITAREKVTRFRS
                     NREGLSDAGRLRIYVSTQTHSSVEKGAGIAGFGRDNLVKVAVRSDFSMDPGALEMAIQ
                     KDIAQGYVPCCAAATLGTTGTTAIDPLREIGVICRKHGIWLHVDAALGGTALLLPEMR
                     WMADGIEYVDSLVFNPHKWMFTNFDCSAYFVRDAASLIRSFEILPEYLKTRTRGEVND
                     YRDWGIPLGRRFRALKLWAVIRSYGVEGLQSAIRGHIRLAKMLAGLIEADRNFGLMAP
                     VPLNTVCFRYCPAGMSDKDANTLNEKINHALNDTGKIYLTHTKVEGRYVLRMVTAQTG
                     VAERHILQAWDLIRETASALSSQDGAVDVD"
     gene            complement(9190..9438)
                     /gene="atpC"
                     /locus_tag="PKK03_02125"
     CDS             complement(9190..9438)
                     /gene="atpC"
                     /locus_tag="PKK03_02125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNX83218.1"
                     /translation="MKIEIITPDRKIYTGEIRSVRVPGRKGSFQVLKDHAPIISTLDA
                     GPVIIADDQGTEVRFEITGGVIEVKRNRIILLADSVVQ"
     gene            complement(9457..10965)
                     /gene="atpD"
                     /locus_tag="PKK03_02130"
     CDS             complement(9457..10965)
                     /gene="atpD"
                     /locus_tag="PKK03_02130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNX83219.1"
                     /translation="MAKKTGVISQVIGPVVDVTFDMAGTDMPNILDALEIKRHDGSVL
                     VVECQQHIGEKTVRAIAMDSTDGLQRGMEVVATGQPIVMPVGDQIRGRLMNVTGMPID
                     GIGPLDKKGGYPIHRKPPKYEDLSTEKEVLYTGIKVIDLIEPYSKGGKIGLFGGAGVG
                     KTVVILELINNIAKAYSGLSVFAGVGERTREGNDLLREMLEANVISYGKEFLDDMEKG
                     GWNLDLVDREVLKESKLALVFGQMNEPPGARARVALSGLSVAEYFRDGEGEGTGRDIL
                     FFMDNVFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGVMQERITSTRHGSITSVQAV
                     YVPADDLTDPAPATTFAHLDATTVLSRKISELGIYPAVDPLDSTSRILTPEIVGEEHY
                     RCAQRVKEILQRYNELQDIIAILGMDELSEEDKLVVHRARRVQRFLSQPFHVAEQFTN
                     IPGKLVSIEDTIRGFNMIIDGAVDQYPEAAFMLVGTIEDAIAKGEKLIAESR"
     gene            complement(11041..12015)
                     /locus_tag="PKK03_02135"
     CDS             complement(11041..12015)
                     /locus_tag="PKK03_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNX83220.1"
                     /translation="MFTSEYLRELTDKAIASLGGTPEADRLFEPVRYIMSIGGKRLRP
                     VMCLLSCNIFRDKIDEAVMPAVGIEVFHNFTLIHDDIIDKAEVRRGSPTVHAKWGPDQ
                     AVLSGDVMAFVAVECIGQAPEKALPKALKLFNRTAIEVCTGQQLDLDFEKKGVVSEAE
                     YLRMIELKTAVLVAASLKMGAIIGDASEKDQDLLYEFGRCLGIAFQIQDDVLDTYGDT
                     KLFGKKTGSDIVANKKTYLLVKALEMAGGSRLTRLRKLLGPGNNDPEAKVRDVRAVYD
                     ELEIRLQAENLAYDYFNRAFQALDQVSVNGERKNELKQLAVSLIGRVK"
     gene            complement(12031..12918)
                     /gene="atpG"
                     /locus_tag="PKK03_02140"
     CDS             complement(12031..12918)
                     /gene="atpG"
                     /locus_tag="PKK03_02140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNX83221.1"
                     /translation="MANLKAIRVRLSSVKSTRQITSAMKMVAAAKLRKAQDAIVQLRP
                     YAEKLNEIQAGLSAALRDTETDNIYAGRKKGNGKVLLVVVTSNKGLCGAFNANVIRET
                     RRIMAEKYSQEARRGDLAVMTVGKKANDALRKLKCHIIEDHSDLYTGLTFDRVSALAD
                     KLMRLYVSKEYDTIEIVYNQFKNAAVQKLTDEVFLPVTMEVSSGASAAPADYIYEPDQ
                     VSIVSELIPKTLRIKLYRSLLDSFAAEHGARMTAMHQATDNATELIRDLTLQYNKARQ
                     ASITNQLLEVVSGAEALNG"
     gene            complement(12941..14527)
                     /gene="atpA"
                     /locus_tag="PKK03_02145"
     CDS             complement(12941..14527)
                     /gene="atpA"
                     /locus_tag="PKK03_02145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNX83222.1"
                     /translation="MTNIKPSEVSQILRQQLEGVKTAIDVEEVGTVLQVGDGIARIYG
                     LKNVGSNELIEFVESNIKGIVLNLEEDNVGAVILGPSEKIKEGDIARRTGRISSIMVG
                     EGLLGRVITTTGDPIDGRGPVTGELYEMPFERKAPGVIFRHPVKQPVQTGLKAIDAMI
                     PIGRGQRELIIGDRQTGKTAIAIDTIINQKSEYDKGKPVYCIYVAIGQKGSTVANIAT
                     TLEQYGAMDYTVIVSATAADSAAAQFYAPFAGAAIGEFFRDTGRDALIIYDDLSKQAV
                     SYREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKIIGSDDVAARMNDLPDSIRHLVK
                     GGGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLESSLFNAGIRPAINVGISVSRV
                     GGNAQIKSMKKVAGTLKVDQAQYRELEAFSKFGSDLDPATLAVLDKGAKNVEILKQNQ
                     YSPVKVEEQVAIIYCGTKGLLRDVPVHKVRLFENDFLAFMRDNHGDVLETIKNGIIND
                     EVTGVLDRAAAEVASRYQSR"
     gene            complement(14546..15097)
                     /gene="atpH"
                     /locus_tag="PKK03_02150"
     CDS             complement(14546..15097)
                     /gene="atpH"
                     /locus_tag="PKK03_02150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNX83223.1"
                     /translation="MNSARIAVRYAKALFDLALEGGTVDGVYRDMKALSRLCAMEEVK
                     GVIRNPVIPQQKRKDAIIALAGDDAEKLTVDFIGLMFSHGRGDYLAAAARNFIDLTRR
                     HRGIRQVTVTTAVPVDDATKKEMAALIAGEKGGTIEFNEQIDESIIGGFILRVDDSYI
                     DASVRNRLNRFRKEFSLAGYAEK"
     gene            complement(15102..15614)
                     /gene="atpF"
                     /locus_tag="PKK03_02155"
     CDS             complement(15102..15614)
                     /gene="atpF"
                     /locus_tag="PKK03_02155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNX83224.1"
                     /translation="MLLQSSLASPAIGTVFWTTLIFLILLVLLWKFAWGPIMKAVKAR
                     EDMINNALESAEKAREEMKVLQADNEIILRKAREERDKILRDARVAYDRMMAEAREKG
                     QSESDALVRRARELIEREKVSAIAEVKKEVARLAIEVASKVVGETLKSDTEQQKLIER
                     YINDIEANRN"
     gene            complement(15680..15901)
                     /gene="atpE"
                     /locus_tag="PKK03_02160"
     CDS             complement(15680..15901)
                     /gene="atpE"
                     /locus_tag="PKK03_02160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNX83225.1"
                     /translation="MEEAQAVMSLAAVGAGLAVIGAGLGIGRIGGSAMDAIARQPEAY
                     SKIQLAMIISAALIEGAALFAIVVAMIKG"
     gene            complement(15940..17016)
                     /gene="atpB"
                     /locus_tag="PKK03_02165"
     CDS             complement(15940..17016)
                     /gene="atpB"
                     /locus_tag="PKK03_02165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNX83226.1"
                     /translation="MKNRYLYLLVLVLGLLVCTVPAASSGTMDEEGKAHGGEEVFDAA
                     PFIMEHVADSHEWHILTKKDGTSVAIYLPVIIYDRESGVHFFSSRKIAHGHSYNGFAI
                     AHEGKYAGKIIRVDQEGHHDGSKPPLDFSVTKAVFGMLVAAVILLWLMLSLARTYKKA
                     GTAAPKGLQGVLEPVILFIRDDIAIPNIGDHKYMKFMPYLLTVFFFILINNLLGLVPI
                     FPFGANVTGNVNVTFVLALFTFLITQFSANKSHWRHVFAAPGVPFWLLPIMIPIEIIG
                     LFSKPFALMIRLFANITAGHIIVLSLVTLIFIFKTVLVAPVSIFFVLFMDCIELLVAF
                     LQAYVFTLLSALFIGMATAEPEHH"
     gene            complement(17028..17420)
                     /locus_tag="PKK03_02170"
     CDS             complement(17028..17420)
                     /locus_tag="PKK03_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83227.1"
                     /translation="MFKPLAVRFYFGLAAIAMFFIIMESVLGRFYPSLFNTSAAAFLT
                     AGFTLAAFISLLIFFNGYHSDRERSVFMTLIALGVKMLLSFVLALLFFLVFKNDATGS
                     VILFFILYLAFTIYVVLTFTGVLKKKSD"
     gene            complement(17422..17664)
                     /locus_tag="PKK03_02175"
     CDS             complement(17422..17664)
                     /locus_tag="PKK03_02175"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNX83228.1"
                     /translation="MEQQQPDQKKKENKGREGLDAYSRYSTIAIQMVVIILITSLGGV
                     KLDKLAGTEPILTVVLSLLGVAAAMWLVIKEALRNK"
     gene            complement(17624..18016)
                     /locus_tag="PKK03_02180"
     CDS             complement(17624..18016)
                     /locus_tag="PKK03_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNX83229.1"
                     /translation="MAKIMETDNGAAVNLISQGTEITGDIKSTGDIRIDGILNGNMNT
                     KGKVVIGPTGKVKGEVECKNSEVSGLIDGKITVSQLLNLKASSKINGTIITNKLSIEP
                     GAVFTGNCVMKEGDNGGTAAARPKEEGK"
     gene            complement(18137..19135)
                     /locus_tag="PKK03_02185"
     CDS             complement(18137..19135)
                     /locus_tag="PKK03_02185"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNX83230.1"
                     /translation="MTKIRAAITGINAWTPEYRLTNQELSEMVETTDEWIMQRVGIKE
                     RRILKGEGKGTSDLGEPAVRGLLEKTGTRPEEIDLLICATVTPDMAFPATANIISDKV
                     GIKNAFSFDIGAACSGFLFALQTAAAYVETGRYKKVIVVGTDKMSSITDYTDRTTCTL
                     FGDAAGAVLLEPTYEEFGVMDYIFKTDGSGRKYLHMKAGGSLRPASHDTVGGREHYIY
                     QEGQAVFKFAVSNMADVAAEIMEKNNLGPDDIAWLVPHQANLRIIDATGRRMGLPPEK
                     VMINIQNYGNTTAATIPLVLWEFEKKLKKGDNLILAAFGGGFTWGSIWLRWAYDPA"
     gene            complement(19132..20076)
                     /locus_tag="PKK03_02190"
     CDS             complement(19132..20076)
                     /locus_tag="PKK03_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HNX83231.1"
                     /translation="MKIGLDAMGGDYAPNAVVEGAFDALGQLPSGDRIVLIGDNRRIR
                     EKLALLDADASLFEIVPTSQVIEMSDHPAKAFSQKRDSSIAVGFGMLKSGLIDGFASA
                     GNTGAMMVGASYTVNVIPGVYRPALAALIPNVNGKSSIILDVGINPDSKPDVLLQYGM
                     LGSVYAKHVLGIKEPTVGLLNIGKEKAKGSAAVKAAHELLNEANDVNFRGNIEGHHLF
                     TDEMTDVIVCDGFVGNVVLKMAEKFYVVARSKGIKDSFFDRLNFEVVGGTPVLGINEN
                     VVVGHGISNRMAIMNMLLQTRDVVHADLAGKIREAFGA"
     gene            complement(20105..20290)
                     /gene="rpmF"
                     /locus_tag="PKK03_02195"
     CDS             complement(20105..20290)
                     /gene="rpmF"
                     /locus_tag="PKK03_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNX83232.1"
                     /translation="MPNPKHRHSSTRRDKRRTHYKAVAPTTAACPNCGSAHLYHRVCP
                     ECGYYRGKLAVEKKTTA"
     gene            complement(20308..20829)
                     /locus_tag="PKK03_02200"
     CDS             complement(20308..20829)
                     /locus_tag="PKK03_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNX83233.1"
                     /translation="MPGLYSIPLAGLKEARYTYEFEIGNHFFGSFEESEIKRGELRAV
                     VVLQKCSTHIELDIVINGKAEVMCDRCLEAFYMPVSTANRLFIRQGREWDESDPDMIT
                     MPLDEHEIDLSQFFYEYIHLALPIKRIHPDDADGNSTCNPDMIRKLNDLLVHGEEETD
                     PRWDDLKKLTGNN"
     gene            21011..21346
                     /locus_tag="PKK03_02205"
     CDS             21011..21346
                     /locus_tag="PKK03_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83234.1"
                     /translation="MKEKHGNPFAGIAKFLISLGLIMIVAKLDLLGLGDIPDYFKWQM
                     LLIFFGLWSLISLELVSAVVLFSIGFWFLMPEMAIQLTEPYKKIYWPAVMVLCGVAFM
                     LKPLTKSCK"
     gene            21372..22112
                     /locus_tag="PKK03_02210"
     CDS             21372..22112
                     /locus_tag="PKK03_02210"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HNX83235.1"
                     /translation="MENELKKPARNPETSNRLIIGVLLIIAGLILIVKKSTMLPEPLD
                     HFIDDIIFSWQMLLIVIGVIALTGSDNKTPGIVMISVGGFFLIPELFTDFFRSFNFFW
                     PALFIVLGVVLLVNSKRLVKKLDFSTVNKADYIDYVNIFSGAERQLITENFQGGKITS
                     IFGGGEVDLTRSSMAPGDNVIEITCIFGGTTIIVPESWNVVIEVTPILGGFSDSRKMR
                     GDVIRDNTRSLTIRGTVIFGGGEIKSYK"
     gene            22116..23174
                     /locus_tag="PKK03_02215"
     CDS             22116..23174
                     /locus_tag="PKK03_02215"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNX83236.1"
                     /translation="MKHPLLENRTRLLVWWLAWLILAAGQSLLIHFGYGSPADVAIAD
                     GVISMVIFGLLGLAVWFPVRFLLKEDNQVYTTVVNVLLTGSLTILAWLFGTKFLVRTL
                     VTEKVDYDIFWHSVLVFRATAGVLIFFVMLLVYYLFLSASRLAEKVARQAQLEAQVRE
                     GELKMLRSQINPHFLFNSLNSVSSLTVTNPLKARDMIVKLSDFMRYSLSSRNEQPVTL
                     GNEMESLRLYLQIEKVRFGERLSIEEDISPECLPALMPGMLLQPLYENAVKHGVYEST
                     EEVTIRTTVRKQNEMVIITVTNNVDTDSVVTRKGAGIGLKNVSSRLELFFGDKADLTV
                     NHGDDSFTVIVRFPFRKS"
     gene            23194..23946
                     /locus_tag="PKK03_02220"
     CDS             23194..23946
                     /locus_tag="PKK03_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HNX83237.1"
                     /translation="MKTIRALIIEDEAPARELIRFMLADHPEVEIVGECGDGFCGARE
                     IKEKKPDLIFLDIQMPKLTGFELLEVIDEKPEVIFATAFDQYAIRAFELNAVDYLLKP
                     FSKERFDDALAKALGRLEKEAPDHEKIEKLVAAASEAEGFLTRIVFRKGAGIKIIPLN
                     NVYYLAAEDDYVMIYYSEGKALKPKTMKYYEDHLPPGLFMRVHRSYIVNVEQITRLEP
                     YSKDNYVAVLKSGEKIPVSRAGYKMLREKLSF"
     gene            complement(23943..24257)
                     /gene="yidD"
                     /locus_tag="PKK03_02225"
     CDS             complement(23943..24257)
                     /gene="yidD"
                     /locus_tag="PKK03_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNX83238.1"
                     /translation="MLKSAAKILSFLLLLPVWFYRWFISPWTPASCRHVPTCSQYAID
                     ALRLHGPFTGIMISTGRILRCRPGGTHGYDPVPRFIFRRYRGGGSPLRRAPACNRLKD
                     HD"
     gene            24370..25074
                     /locus_tag="PKK03_02230"
     CDS             24370..25074
                     /locus_tag="PKK03_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNX83239.1"
                     /translation="METMFLQNGAYWIIFIVFMILSWVVSARLKSKFRTYSKVPIASG
                     MTGRDVAEKMLRESGVTDVTVHSVAGELTDHYNPLKKTINLSRDVYSGTSVAAAAVAA
                     HETGHALQHAQSYAWLGMRSKLAPVVSFASNWIQWVLIAGLVLLNSRPEIMLIGIVLF
                     AITTLFSFITLPVEINASKRALAWLSNAGITYSGTDDKAQDALKWAAYTYVIAALGSL
                     ATLIYYIMLFLGARDR"
     gene            complement(25103..25837)
                     /locus_tag="PKK03_02235"
     CDS             complement(25103..25837)
                     /locus_tag="PKK03_02235"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNX83240.1"
                     /translation="MSLVHELEKSGNWLFRRRSWLPATILVPAAVYLFIANRSVIYFN
                     YTWEIVCILVSVAGELLRSYTVGYVPRNTSGRNVIDQLADDLNTKGIYSVIRHPLYAG
                     NFLMWLGPVLFLRSVWWVIVFVLAYWLYYERIIFAEEQYLRRKFGETYDTWAFRVKAV
                     LPSFRHYIKSDLPFSFRNVLKREYNSITNLFLVFAFLDLCRNIAVTGRIYLEPMYITL
                     LVAALIFWAVIRYLVKRTKFLYVEGR"
     gene            complement(25872..27107)
                     /locus_tag="PKK03_02240"
     CDS             complement(25872..27107)
                     /locus_tag="PKK03_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83241.1"
                     /translation="MRGQTTGNNPDTDELYGMKRIFLTMILVTSVAAAVYSQEAVSDD
                     PGAGSGKTDRLTAGGYASWLHTAIFDKPSENWINTGMLHNRLNFKVYAGSRLTFALEI
                     RNRFVTGDMVSLDPSYIDGLSADAGWADLSWNISSGSSYVLNTMIDRAYVDFTAGQLQ
                     IRAGRQRINWSQALIWNPNDLFNTYSFFDFDYVERPGSDALRFIWSTGPSSAVEAAVK
                     VDGNNRITAAALGRFSVLNTDIQFLAGVTDDEYFTAGTGWSGAIGSVSLRGEATLFAP
                     FDGTGGEKSNFITTLGIDKAFSDKVTALAQVMYSSHPADLSGFTDLYGGGLTARQLAF
                     SEFSAAAQVTFVPLPLVNLSLAAIWFPDLDGFYAGPSLDFSLAENVDFSILWQHFNST
                     FGEEKTRINLGFIRIRYSF"
     gene            complement(27082..27777)
                     /locus_tag="PKK03_02245"
     CDS             complement(27082..27777)
                     /locus_tag="PKK03_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX83242.1"
                     /translation="MNVVEINDVYKTYHDTEVEVRAVNGVTLSFTKGDFAVIVGPSGS
                     GKTTLLNLIGGLDQPTTGEIIVAGHRLSELKPSELIDFRMEHVGFVFQSYNLIPVLTA
                     AENVGFIMSLQKWPKEKRMARTGELLEAVGLADRHNSRPSQLSGGQQQRVAVARALAP
                     RPEFILADEPTANLDSKSAETLLDIMEKLNREEEMTFIFSTHDARVMKRARRVITLED
                     GKVVSDERTNHRE"
     gene            complement(27797..29224)
                     /locus_tag="PKK03_02250"
     CDS             complement(27797..29224)
                     /locus_tag="PKK03_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNX83243.1"
                     /translation="MITSIAWKNVWRNRTRSLTVIAAVTVGVFASVFALAAINSSIVQ
                     RIDSAVNEELSHIQVNTKEFRSSSDILNIISEPDKVAETLRKTPGVTDVTGRIIVRGI
                     ASAPSGSAGVEITGIDIEKEKQMFTLSGKLIPGTGSYFGADTKLNSAFIGEKLARELQ
                     IIRYILTAESLTALVDKGVPSAVTDRLKPLEGQRFTTEKKLTEAYYRLLTPKEIKKYG
                     LAIREAAWSYREGSRVILSFLDVNGNQTGAAYRICGIFRTNNDLFEATSVFVPMEELR
                     RLTGLEDGVYHRFIARLEENDMTDRVTPVLKEALPGLEVMNWKEIQVDLAMIADYINQ
                     IYAIFMVLILAALSFGIVNTMLMAVLERTRELGMLSAVGMNRRRIFLMIMLESVFLSV
                     VGGFTGMAVSGIVIGITNHTGINLVKYSEGLEAFGYSAHLYPTIGADFFIILTVLIVI
                     TGILSAVYPARKALQLNPVEALRGE"
     gene            complement(29236..30651)
                     /locus_tag="PKK03_02255"
     CDS             complement(29236..30651)
                     /locus_tag="PKK03_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNX83244.1"
                     /translation="MKDRQLAWRNLWRNRKRTLITAASVFFAIFFALIMRSFQLGTYD
                     KMYSDVIRSYSGYLQLQHPSYLDEPGLDNGLILDPELTRKLEQDPNVEAVVPRIESFA
                     LAAAGNRTQGVMVIGVEPERESKALNIRNRIVRYKLTPGAVEALKGEELPGRLAKNTE
                     LFAGEAFTEAEDLMSVLGISKKDSAGLLPLLRNHTSFPGSYLSAGESGYAIIGNGLSD
                     YLEAVPGDTIVLIGQGYHGTSAAGLYVVKGVVSMPNPEIDNRFVCLTLDDARWLYGAP
                     DMATSAIIAIKDGSDKVLTATQQRLGAVTGEDLAVRNWKEMNALLLNQMEADNRSGAI
                     MIGILYLVIAFGVFGTVLMMMAERRREFGMLVSIGMRKGRLAGIVGLEMLLMGVIGVI
                     AGVIASLPIVFLGHLRPLRFTGEMARMYEDYGFEPVMPMLLPDTYYLWQVVVVLLILA
                     VAIAFSIRKIFKLNVINALRA"
     gene            complement(30679..31455)
                     /locus_tag="PKK03_02260"
     CDS             complement(30679..31455)
                     /locus_tag="PKK03_02260"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNX83245.1"
                     /translation="MKGIPGQTAASLFVLIMLTAVSLPAASQLLSAKEIVTKADEKFN
                     GEKSGFSVMSMTIVRPTWQRTVEFKSWSLGDDYALTLITAPAREKGQTFLKRLTEMWS
                     WNPAISRLIKLPPSMMSQGWMGSDYTNDDILRESSVVNDYDHSLEGEEVVDGRLCHKI
                     RLTAREDAGVLWSHQMWWVDRKDFIVLKAELYDEDGYLVRTERGMDLKTMDGRLIPTT
                     IELVPAEQEGHKTILKITEIRFNISVEESFFSQQNMRTIR"
     gene            complement(31452..32084)
                     /locus_tag="PKK03_02265"
     CDS             complement(31452..32084)
                     /locus_tag="PKK03_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX83246.1"
                     /translation="MSPRSSAQFEEMREEKKALIMNTALEHFSDYGFHATTISHIAKH
                     AGISKGLMYNYFKSKEELLAAIINRSLDEIYRSFNPDRDSTLTPDEFENFIRRIFAIF
                     RENRQFWKLIYRVMLQRGVYEHLSDDPDATFTVGGIPLMEYSAYMAKLMSDYFIRKKE
                     TEGPDFDPVTEMLMFSNTLKGFALTYIFAPGQYPDEYFEKMVETLIRKYR"
     gene            complement(32524..34536)
                     /locus_tag="PKK03_02270"
     CDS             complement(32524..34536)
                     /locus_tag="PKK03_02270"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNX83247.1"
                     /translation="MEQVALYLLKSAAWLAGFTLLYLLFLRRERFFMLKRVYLMTGVV
                     LSILLPLVTLHYRVDLPAPLIQDTQGMASVFPSPAAASAEVTENADPLKWFMLFYAAG
                     ILFLLVRTLKYLFSVYRTVRIGPVNREGDALLVRTEKYTSAFSFFNYVFINPSLSGRE
                     VREILNHEMVHLRQKHWFDLLLVEVMSLMQWVNPFAWICSALVRQNHEHLADEEALQR
                     SSDPAGYRATLLNQVFSARLISLSNSFNFSFNTNRFEMMKKKNYSPCRKLKLLLVLPL
                     TALMMYAFADPVYTYSGKSDSQTAGMIKDIHGTVVKEDGTPLPGVTVNVSGTPLSVTT
                     DESGKFYFSDLPGYAALIFTASGYEPVSITAESARGISVTMKANPLFTTRSPSSGAEI
                     VVIDGIVSDLSLEEVTKRLGKDFGKYTYLMPEAAKAKYGEKGAKGAFEVISRSKAKES
                     GLNLPYPRKVETDFPTFRGESYLAFADWVAEKANYPQEAAAKNISGWARMTYTVETDG
                     SVSQVKPSSDTDPLFAAELTRVMESSPRWKSPANKDANEPFTSEILIRFELPGTVSNG
                     IVYIMVDEMPKFPGGDAALFEFLYKTIQYPAQAKDNGIHGKVILRFIVTSEGNVENVT
                     VVRDVDPLLDNEAVRVMRLCPKWEPGKMKGKPVNIYYSVPISFALK"
     gene            complement(34540..34914)
                     /locus_tag="PKK03_02275"
     CDS             complement(34540..34914)
                     /locus_tag="PKK03_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNX83248.1"
                     /translation="MQLTRAEEQIMQILWDLEEGVVKDIREKFDDPKPARNTISTVLK
                     VLEKKGYVDHRAYANVFLYYPLVSKREYSKNQLFGLLEGYFNNSFPAMTSFFAREKNL
                     SVKDLDRILEEAREELDDDQQE"
     gene            complement(34940..36403)
                     /locus_tag="PKK03_02280"
     CDS             complement(34940..36403)
                     /locus_tag="PKK03_02280"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNX83249.1"
                     /translation="MAIFISVLVLLTSCKDGKDYSPEIDFGGVAYVKVIVQDDLDSVR
                     MDINHGSYYPIQSCLIHRFTLTERRVCEASYSITRPELVWFEFRDTVFTSYMRPGDTL
                     VARVGLDEAGDDNRKISFQMDDPIFAFLQNERSEFGCFYTQSPLAIMAFNTQPETERQ
                     FEDAIKKIDQARQERLKFLEKNWDILPEWFCDLYENEISYFSENMKFYQYYYLKNRNL
                     GSILMPCDVKIYNPEAVLSPLYWRFISDYFLLSDSVDYRLIGPPRLMAYYSKASHNIN
                     SLLKGKILEYFNAYFLYDLYYGADTRKELQLVDSFRIATDFRLTSLQESYIDDRKSQS
                     LKKIEKLEDGNRLKPGSEAPYFYLKDSAGVFHTLSDYRGRLIYLHFWATWCGPCLEEI
                     PSLNKLAENLSGKPIEIINICLDDEYEKWETIIENEKLKGINLICVGSWAADLQEKYS
                     VRGIPHYAIIDTDGLIISENCERPDKVYERLLTLIQQ"
     gene            complement(36611..37804)
                     /locus_tag="PKK03_02285"
     CDS             complement(36611..37804)
                     /locus_tag="PKK03_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX83250.1"
                     /translation="MESILELSESRVTNIFAKQARYLMNQIPGDQRLVLVRGSRGTGK
                     TTLLLQFLRSLGLPSSRAAFISLDDIFFSRHPAEDFTDWFVKRGGEYLFIDEVHRNSH
                     WSLLVKSLYDRYPNLKLLISGSSALQMEKGETDLSRRMLTLNLNELSLREFISMKYDI
                     DLPAISIDELLIDHERIAREVLSYVKPLMIFDEYLKYGAYPYFLEGIRYYHERLSNTV
                     SAVLDSDLPAVSGITYGTILKIRKLLSVLAEAVPFTPNINELSGRVEISRDLLYRYLH
                     LLEQAGLIYQLRRDSRGISIMSKPEKIYLHNTNLSQALTLHKPDKGSERETFFLNQVS
                     ARYTVNYPDEGDFIVDGKYTVEVGGRKKSGKQIAKAENAIVVKDDIEYGSGNIIPLWL
                     FGFLY"
     gene            complement(37940..38827)
                     /locus_tag="PKK03_02290"
     CDS             complement(37940..38827)
                     /locus_tag="PKK03_02290"
                     /inference="COORDINATES: protein motif:HMM:NF015709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HNX83251.1"
                     /translation="MKKLIIVTLIIMLASCATVKQTSYPAEDRSAPSAGNLAAEDSQD
                     DQDNLILATLWFQKSAEMRALFYQCFRNAEVALRENLEMHNEGKPAADSSIPADSGQP
                     GSEKPAAVVLDIDETLLDNSPFQGWQVLRKKAFNNDDWFRWVELARARPLPGAVEFTR
                     YADSLGVEVFYVSNRTVGEMGPTISNMAAWGFANADSTHMLLKETVSSKVERRALIEK
                     DYEILLLVGDNLADLSGIYEKRGPDHGFGAVDADRRLFGTRYIVLPNPMYGNWLNDLL
                     RSAPGSTEREKLLNMLEGF"
BASE COUNT         9426 a        10331 c         9409 g         9857 t
ORIGIN      
        1 aagaaactcc cgtacccgcc gtgccggcac cctgagacct tcagacctca gaccttctga
       61 ccttcagacc ttcagacctc agaccttcag acctcagacc ttcagacctt cagacctttc
      121 cgggacaccc cttttcgaag agggaaatag tctccgactg aacaaggacc catatgatgt
      181 agactccctt gagggtgccg atgggtacgt tgagcaggcc catggccccc aataccagtg
      241 tcagtatcct ggcccacggc ttgcagtaga agaggccgat gccgccggca atcattcctc
      301 cgccgaaaag caacatcagg acaaccagcg gggttgagat tattccgagg accttaatgg
      361 ctgtaggatc atcaacaaac tggtcaatga atccgaacag gactgcaatt gtcagtgcgc
      421 caagaacaag gaatgagccg aaaactatct gcagtatacc agcaatattg acatgcttct
      481 tcatcctgtt ctcttcaact gtcaccactg tgggtgatgc ggcattctct tccataatat
      541 gaacgtttta ggttttctgt tttcagaata atgactccaa tttatgaaaa atgctgcaca
      601 tggcctgcca tactaattgc ttacctttgc cgttaattca gaaatgtcag ccatgaaaaa
      661 attactttac gcttccgctt gtcttataat cacctgttca ttcaattcct gcgaaatcct
      721 gggtgacgga tgccaggtat gccgtacggt ctcttatgag aacggcaatg ctattgcctg
      781 gggtaccgag gctgaatact gcggacagga actgataagc attaaggcaa tccctccctc
      841 cactgtaagt ggggtaacaa cctactggga gtgtcgctga ccctcctata acgctgattt
      901 tatgagcatt ctccaactct tgatttcctg tttaaaagtt gttgttaata agttgttctt
      961 aaagttgaca tttgcggcag aaatgtttaa tcttgcttgt cctcgccgga tatctttacg
     1021 tggagttcgg attattaacc ccgggttcac tttataaaca tgcttaacat tacaggcaag
     1081 cggatagcta ttgttgagga cgatgctgca tccctgaagt attatgagac gctgctcaag
     1141 gagaccggcg ccgatgtttt tacctaccgc aacggaaagg agtttgttga ggctataact
     1201 acaggcaggg aacattttga tcttgttttc atggattttc ttgtgccgct ggtaaacggc
     1261 atcgaatgca ccagggcctt ccgccaggtt aaccgctctg caccggtgat tctcatcact
     1321 gcatattatt ccgaacaaac caaaaacgaa gcatatatag ccggctgtac ggaatacaac
     1381 ctcaaaccca tattccctga caagatccac ctgattcttg agaagtacct ggctcccagg
     1441 ccggcctatc agatgtacga ctgattcaca gccaccagtc gtcagccggc tgccggctgc
     1501 cgaactggtc cttatcctgc cttactgctg actgccaact gcaggttgcc tactgattta
     1561 aagcggcata tctggcataa agctcattgt attttccgtt gatatcttcg gcaagtgcta
     1621 tttcgccgtt atcatagcag actttaatag cctggagcat catctgaagg ccggtggcaa
     1681 tttcagcacc ggcataataa gcgacagacg gcctctggtc aagcaggtat cctgcccgtt
     1741 ctgcataata atcagcaaac tcacctgcca gctccttcgc cttatccttc cgtccggttg
     1801 caaaacaagc cgagataata tcaccgaaat aatagtcata gtctacattt gataccggga
     1861 agagctcaag acacctgtca attacctgca ccgccctggc agtatcaccc tcagccacaa
     1921 gtgccccggc aagcctggca tgattcaggc gtgccctgac cacgaggagt gtccggttat
     1981 gataaaagtc aatgtaaaca tccggatcat tggcaccctt ccagctgaag cggttcatca
     2041 tgttctcgta gagaatgtca gtatcgatgc ggccgtactc aaaccagctc ttgttgaccc
     2101 cttttacggg caccagcctg tacgcaagtc cttcaagctg gaagtattcc tccagaccga
     2161 gggcgtcatc agtctggccg gtgacaaaat agatcggacg gtcccagtcg ttgtgagcca
     2221 ggatatcaag caccaggagc tggctcttca ttatccatga acctttgagc gtaatgtcaa
     2281 tatagggcac aatcttgtca gcatcctccg gtttgactgt gccattggcc agcaccttcg
     2341 cggaatcaac cggtatgcgt atggtgcggg tgggtatata ccacatgtcc tctccgtaca
     2401 tcttgagctt tgacaactcg ctgccgctgg aaatgaattg catcacctct tttgcagttg
     2461 cgggctccct caccttctcg agaacggcaa cctggttatt gactccgtca tagtaaagtt
     2521 ctttctgcag gctgaccggc aatggttcag aagtgtaagc cttgtatttc atctggtcaa
     2581 tgtaccagtc agtgttgagc aacatcaggt tgcagaccct gacatcagtg cggactccct
     2641 ccacctcctg ggcgtaccac agcgggaacg tgtcattgtc tcctccggta aaaattatag
     2701 cattcggagc gcatgaattg aggtagttga aggctacatc ccttgccagg tatctccccg
     2761 agcggtcatg gtcatcccag ttctgcgatc ccatgagggc aggcacagcg gcaaaggtca
     2821 gcacagaggc aatgacggct gcaggcgtct ccccggctgc cttcctgaac agctcaaaaa
     2881 gagccagcac cccgaggccg acccatacag taaaggcata gaatgatgcc gcataggcat
     2941 aatcgcgttc acggggctgg ttcgggtact ggttgaggta tatgacaata gctataccag
     3001 tcattacaaa cagcagaagc actatccacc agttgcgcac atcgcggtta aagtgccaca
     3061 ggaaacccac cagcccgaga agaagaggca gcaggtaata cttgttgcgc gacggatcat
     3121 tacgcatatc ttcaggcatc ccttttaccg atccggtccg gggggcatcc aggaaatcaa
     3181 gccccgttat ccagtttccg ttgaccggcc ctccggtact ctgaacatca ttctgccttc
     3241 cgctgaagtt ccacatgaag taccggaaat acatgaatcc cagctgatga cggaacatga
     3301 acccaaggtt ctgtccgaac gtcggcaccc gcagcgtttt tgcctccccg gtctgggggt
     3361 cattgatccg gacaggtttt cccttcgtac caacatatct ttcatactcc cttacatgat
     3421 ccggagaatc actccacatc ctcgggaaga cagtcataaa cctctcatca tactccgttt
     3481 ctgtctcgcg gctggtgata atatacctgt cgccggaagg attgtaagtc ggcttcccct
     3541 ttgcataatc ggttaccggc gcattgtagt acggcccgta gaaaagcgga cgcctgccgt
     3601 actgctcccg gttaagataa taaagcagcg cgaaggggtt cgaaggatta ttctcattca
     3661 tcggcggatt tgcggtagac cggataagaa tgaccgcaaa gctcgaataa ccgatcatga
     3721 taaccaggag agaggtcatg atggtattca aagccgcttt ctgcttcgtc gagagaagcc
     3781 agacgccata tattattgca gccaggaaaa cgagattgag caacagtgag ccggtaagca
     3841 gccacactcc cgaaaggaga agcgttgtac ctgcaagcag gaatatcctc atcctgtcac
     3901 tgtgtgtgaa gctgtaccat accgacagca ccaacgcggc aatgaagagc aacaggtgga
     3961 tatacatgcc gctgttgaac ggcagcccga ggctgttgac gaagaacagc tcgaatgcgg
     4021 aagtcaccct gacaatacca gggatgatcc cgtacatgat aagcaccagc agcagtatcg
     4081 atacgcctat tgatactgca aaacccttcc atgaaaacct gtacttgcgg aagtaccaga
     4141 taaagacgat ggcaggtatg gcaagcaggt tcagcaggtg tacgccaatg gaaagaccca
     4201 tcatgaaggc gatcagtatg atccatctgt ctgcatgcgg ctcgtctgcc acatcttccc
     4261 atctcagcat tgcccagaat accacagcgg tgaagagcga ggaggtggca taaacttctc
     4321 cctccactgc cgagaaccag aatgtgtcgg agaaggcgta agccagtgaa cctgtcagtc
     4381 cggcaccgag aactgcaatg atccgtcctg cggtgtagtc agattcctgc ctgaggaaga
     4441 tcctgcgggc caggtgggtg atggtccaga aaagaaacag tatcgtcagc gcgctggcca
     4501 gtcctgacat ggcgttgatc atcatggcta cccttgactc atcgcctccg gcgaagttgg
     4561 caaagaacct tccgagtatc atgaaaaacg gagcccccgg ggggtgcccc acctcaagct
     4621 tgaaggccga agcgatgaat tcactgcaat cccagaggct cattgtaggc tcaagtgtca
     4681 acaggtaggt aactgacgcc acaacaaaaa ccgtccagcc aaggatatta tcccaaagcc
     4741 tgtatttctt tatcatattc gtttaatttc cgttccggat aattaccgca cgaaaataat
     4801 aagattttgc cgtgtacccg tagatatcgg ccaattttgg tatttttgga caaaccgccc
     4861 tgcatgaaga gatgtattat tctgctcata atgagcctgc ttttaatggc cagggccgga
     4921 gcgcaatact atgacaccgg acaggatccc gcttctctca ggtggcttca gatacgcaca
     4981 cctcacttca gggtcatcta ccctgacgac ttcggcgggg aagcattccg gtatgcgcgc
     5041 ctcctcgagg aatcatttga aaagctgtct gttctttatc cgggggtaag gacaaacatc
     5101 ccggtaataa tccacagcca ctcgatgcag tcaaacggct atgtgagctg ggcgccccgg
     5161 aggatggaac tgtaccccct gccaggccag gataacctcc cgatgtaccc tgcagcgcag
     5221 cttgcggtgc atgagacggc tcacgtgctc cagctcggct ccctcaacac ccgcggcttt
     5281 ggcagggcgc tgcggttcat cctcggggaa caggcggtgg ggctgagcgc agtgcagata
     5341 cccgcatggg cattcgaggg tgacgcggtc tacgccgaga ccgcaacagg cctctcaggc
     5401 agggggcgga gcaatgcctt catccggggc gccatggcac tgaccctcag accggaagag
     5461 atttacggat acgataaaat gctctcaggc tcataccgga atttcacccc tgaccattat
     5521 gtcttcggat acctcatgat gaaccacctc cggaccattg accccgacac atggaacagg
     5581 gtaacagaaa gggtatcctc aggctatttt cccaatcccg tcaacacagt cctgacaaga
     5641 gagaccggac tcagtaaaag aaagctctat gattctgcct tcgctgccct cgaaagaaac
     5701 tggagcaaaa gcagtcctga aggagtgaag gagtacaccc cgctgagctt aagcaggaga
     5761 aggaattatg tcagccactt caccccctac aggtcggatg acggaaggat aatatccctg
     5821 cggacttccc tgtccgatcc gtcgcggttc gttattaccg aaaatggtgg agggaaagag
     5881 ctgaatatta ccaccaccgg atacatatat ccttactttt tctcgttctc tggtaatact
     5941 atagtatggg cagaacaaca ccctgatata aggtgggata accgtgacta ctcggtgata
     6001 aagaggcttg atctcccggg aggcttaata accgatgtaa ccagccgtac acggtatacc
     6061 gcaccggacc tttcacctga cggacgcacc atagttgccg tttcaaccac ccctgacatg
     6121 gtctgttccc tggttttcct cgactctcat accggagaag tgctgatgaa cctcgttccg
     6181 cccgaaagtc ttatactcca gcgaccggca tggtcatctg acggccgcga agtcaccatg
     6241 gtcaccctga accagcaggg agagggaatc agaacataca ggcccaccgg caagatatgg
     6301 accgtgaacc tggaagagag tcatactgac atagtgcagg cgaagatcca caatgacact
     6361 ctcttcttcc ttgcacaggg agacggctct gacaatattt accgtattgc gggcgccgga
     6421 cctgtggaac gagttacagg ctcccggttc ggaatctccg gattttcagt caccggcagt
     6481 gagctgctct tctctgatta tactgcgggc ggattcgtca ttgcctccga aaagacctca
     6541 gccactgccg gaccggcatt tgtgaccggc catgagattt ttccgccggt ggcgcccatg
     6601 ccaggcaaag ttcccggaaa ggacccgtcc ccgccggtgg tgcatacgcc cgcagctgac
     6661 tccttgagtg acacacccat gccgctaatc cctgaaccca gaccataccg caaaatttca
     6721 aacctgttca atttccatag ctggttcccg ttctatgccg acctggatga gatccggacc
     6781 gatccaacca ccataaggcc gggtctgaca ctgatgtcac agaaccacct gagcaccctt
     6841 atctccaccg tggggtatga atacagtgaa ggcaaccact atctgcactc cggggtcaca
     6901 tggaaaggct ggcacccggt cattgatgca gaggcagaat ggggaggaga ccagctgatc
     6961 ataagtgaca ccagcgagaa ccagccgccg gagaatccgg ggaccgacct gcagctaaac
     7021 ctgagcatct atgaccagtt gtggttcgcg cgcggtaaat tcagacagat gctgatgccc
     7081 gccctataca tcagtttccg caacagggat acctggatat cagccgagaa ccggtttgac
     7141 agggacgtac tcagccttac cggcaggttc tatttctcaa ataccttccg tacggcttac
     7201 cgtgacatca acccgaggtg gggacaggta ttcgatctgc ggctgaccac aacgccatgg
     7261 gacacaaaac tgtacaactc caaaagctat gcccggacaa tcctcttctt tcccggcgca
     7321 ttgtcgaacc acagcctctc cttcagggcc ggctgggaga accaggcccc ggcccggaaa
     7381 cagatttacc gcaacacact tccatggcct cgcggttatg attacgacct ggttgcggaa
     7441 aaactcttct ccttctctgc tgactataca atgccgctct tctatcctga cctggctgca
     7501 ggcagcttcc tgtacctcaa gaggatcagg ggcacactgt tctatgacta ctcaaagggg
     7561 gtggatgtcc gcaactatga tgacaggagc tttaccgccg gagcagactg gttcagctca
     7621 gtgggatcgg agctgatggc tgacttctac gtgctgaggt tcccctttga gataagcgcg
     7681 ggggtacggg cgggctatat gccgcgtgaa gataaatact tcgtgaacgg ggctttttca
     7741 gtcaacatct acggcaccgt cctgggacga gagcgctgac gctgtctccc tgatgagatc
     7801 ccatgcctgc agtatgtgcc gttccgctac gccggtctgg gccgtcacca tcctcagaac
     7861 gtacctgcct tcaacctttg tgtgcgtgag ataaattttc cccgtgtcat tgagggcatg
     7921 gtttattttc tcattcagag tgttggcatc cttatcgctc atgccggcgg ggcagtaccg
     7981 gaagcagacc gtattcagcg gcaccggagc cataagcccg aaattcctgt cggcctcaat
     8041 cagcccggca agcatcttcg ccagccttat atgcccgcgt atcgcagatt gcaggccctc
     8101 aacgccataa gagcggatca ccgcccacag cttcagcgcc ctgaagcgtc tcccgagagg
     8161 gatgccccag tcgcggtaat cattcacctc tcccctggtg cgggtcttaa ggtattcggg
     8221 aagtatctcg aatgagcgga tcagggatgc agcatccctg acaaaatatg cagaacagtc
     8281 gaagttggtg aacatccact tgtggggatt gaatacaagt gagtcaacat attcgatccc
     8341 gtcagccatc cagcgcatct cggggagcag gagggccgtt ccgcccaggg cagcgtcaac
     8401 atgcagccag attccatgtt tacggcagat cacgcctatt tctctcaacg gatcaatggc
     8461 agtggttccc gtggtgccaa gggtggcagc ggcacagcag gggacatacc cttgggcaat
     8521 atctttctgt attgccatct ccagggcccc gggatccatg gaaaagtcac tcctgacagc
     8581 caccttcacc aggttatcgc gtccaaatcc ggcaatccct gcccccttct ccaccgatga
     8641 gtgagtctgg gttgagacat aaatgcggag acggcctgca tcagagagtc cttccctgtt
     8701 ggatcggaaa cgggtgacct tttcccgggc ggttataagc gctgccaggg tagccgatga
     8761 ggcgctgtcc tgaataacac cttcaaattc cggcgggatt cctgtcattg acctgagcca
     8821 ctccatcatc ctctcctcaa gctcagccgc tgccggcgag gtttcccaga gcatgcactg
     8881 cgccccgagg gctgccgtga gcatctcggc aagcaccgaa ggaggactgc tgtttgcggg
     8941 gaaatatgca aagaaattag ggctctgcca atgggtgatg cccggcatga taacctcatc
     9001 aaaatccttc atgaaaagat caaaggattc gcttcctgag ggcggaagct ccggcaatgc
     9061 attctttatc tctcccggtc tgacacgtga ttttaccggg tattgctcca cgttctcaaa
     9121 gtaccgggct atccactcaa cgatctcatg cccgtacctg cgaaactcgt tgctgtccat
     9181 gttactgtgc tattgaacca cggaatcggc caggagaatt atcctgttcc tctttacctc
     9241 aataacgccg ccggtaatct caaacctcac ctcggtgccc tggtcatctg caatgatcac
     9301 cggcccggca tcaagggttg atatgatggg cgcatggtcc ttcagtacct ggaatgagcc
     9361 ctttcttccc ggcactctca ccgagcgtat ctcccccgta tagatcttcc tgtcaggtgt
     9421 aatgatttca atcttcacgt ccggcttttt taatggttac ctgctctctg cgatcagctt
     9481 ttcgcccttg gcgatggcat cctctatggt tccgacgagc atgaaggcag cctcgggata
     9541 ctggtctacg gcgccgtcaa ttatcatgtt gaatcccctg atggtgtctt ctatcgacac
     9601 cagtttaccg ggaatgttgg tgaactgttc ggccacatgg aatggctgtg agaggaacct
     9661 ctgcaccctg cgtgcacggt ggacgacaag cttgtcctcc tcggagagct catccatgcc
     9721 caggatggca atgatgtcct gcaactcatt atatctctgc agtatctcct tgactctctg
     9781 tgcgcagcgg tagtgctcct cgccaacgat ctcgggggta agtattctgg atgttgagtc
     9841 aagagggtct acagccgggt agataccaag ttcggagatc ttacggctca gcaccgtggt
     9901 ggcatcgagg tgggcaaagg ttgttgcagg ggccgggtca gtaaggtcat cggcaggcac
     9961 atagacagcc tgaaccgaag tgattgatcc gtgacgcgta gaggtgatcc tctcctgcat
    10021 cacacccatc tctgttgcaa gggtgggctg gtaacccacg gctgagggca ttcgccccag
    10081 cagggcagac acttctgaac ctgcctgggt gaaacggaag acgttatcca tgaagaagag
    10141 gatatccctt ccagttccct caccctcacc gtcacggaag tattctgcca cggagaggcc
    10201 cgagagagcc acgcgtgcgc gtgcaccggg aggctcgttc atctggccga agacaagagc
    10261 cagcttcgac tccttcagca cctcgcggtc aacgaggtca aggttccagc cgcccttctc
    10321 catatcatca agaaactcct tgccgtatga tattacattg gcttcaagca tctcacggag
    10381 gaggtcattg ccctccctgg tgcgctcacc cacgccggcg aagacggaaa gaccggaata
    10441 ggcctttgca atattgttga taagctcaag gatgacaacg gtctttccca cgcctgctcc
    10501 cccgaagaga ccgatttttc ctcccttgga gtaaggctcg atcaggtcaa taaccttgat
    10561 gccggtataa agcacctcct tctcagtcga caggtcctca tatttcggcg gtttgcggtg
    10621 aatgggatag cctccctttt tgtcaagggg cccgatgccg tcaataggca tgcctgttac
    10681 attcatcagt cgtcctctta tctggtcacc caccggcata actatcggct gcccggtagc
    10741 tacaacctcc atcccgcgct gcagtccgtc ggttgaatcc atggctatgg ccctgacggt
    10801 cttttcacct atgtgctgct gacactccac aaccagcacc gatccgtcat gacgcttgat
    10861 ttcaagcgca tcaaggatgt tcggcatatc ggttccggcc atatcgaagg taacgtcaac
    10921 cacaggcccg atgacctgac ttatcactcc tgttttcttt gccatctctc tgacttaaat
    10981 ctgtttcaat gtaatctgat aatctgctgt ttatatactt tgcccctgac ggggcgaaat
    11041 ttattttacc cttccgatga gactcaccgc aagctgtttc agttcgttct ttctctctcc
    11101 atttaccgat acctggtcaa gcgcctggaa tgccctgtta aaatagtcat aggcaaggtt
    11161 ctcagcctgc agccttattt ccagttcatc atagacagcc ctcacatccc tgactttcgc
    11221 ttccgggtca ttgttgccgg gcccgagaag cttcctgagg cgggtaagcc tgctgccgcc
    11281 ggccatctca agggctttca ccaggaggta ggtcttttta tttgccacta tatcgctgcc
    11341 ggtcttctta ccgaacaact ttgtatcgcc ataggtgtca agaacgtcat cctgaatctg
    11401 gaaggcgatg ccaaggcagc gtccaaattc gtaaagcagg tcctggtctt tttcggaagc
    11461 atctccgatg attgctccca tcttgaggga agcggccacc agcaccgcag tcttcagctc
    11521 tatcatgcga aggtattcgg cttctgacac cacccctttc ttctcaaagt caaggtcgag
    11581 ctgctgaccg gtgcacacct caatggcggt acggttgaag agtttcagcg ccttcggcag
    11641 ggccttttca ggggcctgac ctatgcactc cactgcaacg aatgccatta catcgcctga
    11701 caatacggcc tgatccggac cccacttggc gtgtactgta ggagatcccc gtcttacctc
    11761 agccttgtca atgatatcat catggatcag ggtgaagtta tgaaacacct ctatgcccac
    11821 cgccggcatt actgcctcgt caatcttgtc cctgaagatg ttgcaggaca aaaggcacat
    11881 aaccggtctg agtctctttc ccccgataga catgatgtat cttacgggtt caaagagcct
    11941 gtctgcctcc ggtgtgccac cgaggctggc tattgccttg tctgtcagtt ctctcaggta
    12001 ttcgctggtg aacatgtctg tctccttccg ttatccgttt agcgcttcag cgccgctgac
    12061 cacctcgagc agctggttgg tgatggaggc ctggcgcgcc ttgttgtact gcagggtaag
    12121 gtccctgata agttccgtgg cattgtccgt agcctgatgc atggcagtca tccgtgcacc
    12181 gtgctcagca gcgaacgagt caagaagtga gcggtacagc tttatgcgca atgtcttcgg
    12241 aatgagttca gagacgatgc tgacctggtc aggctcgtat atatagtctg caggcgctgc
    12301 ggaagctcct gacgaaacct ccattgtcac tggcaggaac acctcatcgg tgagcttctg
    12361 taccgctgca tttttaaact ggttgtaaac tatctcgatg gtatcatact ccttcgaaac
    12421 gtagagtctc atcagtttgt cagccagcgc cgagaccctg tcaaatgtca gacccgtgta
    12481 aaggtcactg tgatcctcaa tgatgtggca cttaagcttc ctgagcgcgt cattcgcctt
    12541 cttccccact gtcatcaccg ccagatcacc cctcctggcc tcctggctgt atttttcggc
    12601 catgatccgc ctggtttccc tgatcacgtt ggcattgaat gccccgcaaa gccccttgtt
    12661 ggatgtgacc accacaagca gaacctttcc gttgcccttt ttccttccgg catatatgtt
    12721 atcggtctcg gtgtcgcgca atgcagcgct gagcccggcc tggatctcat tgagcttttc
    12781 ggcataaggc ctgagctgca ctatcgcgtc ctgtgccttg cgcagcttgg cggcagccac
    12841 catcttcatc gcagaggtga tctgcctggt tgatttgacc gagctcagcc tgacccttat
    12901 cgctttcaga ttagccatcc tgtttatcta tcctttgaga ctagcgtgac tggtaccgtg
    12961 atgcaacctc agctgcagcc ctgtcaagca caccggtcac ttcgtcattg atgattccgt
    13021 ttttgatggt ttcaagcacg tcaccgtgat tgtcacgcat gaacgccaga aagtcgtttt
    13081 caaacagcct taccttatgc accggcacat ccctgagcaa tcccttcgtg ccgcagtaaa
    13141 tgatggctac ctgctcctca accttcaccg gtgagtactg gttctgcttc aggatctcaa
    13201 cgttcttggc gcccttgtca agcactgcaa gggtagcggg atcaaggtca gaaccgaatt
    13261 tcgagaaggc ttccagctca cggtattgtg cctggtcaac cttaagcgtt ccggccactt
    13321 tcttcattga tttgatctgg gcgttacctc ctacccttga caccgatatg cccacgttga
    13381 ttgcgggcct gatgcctgcg ttgaagaggc ttgactccag gaatatctgg ccgtcagtga
    13441 ttgatatcac gttggtcggg atatatgctg agacgtcacc tgcctgggtt tcgatgatcg
    13501 ggagggcggt gagtgatccg ccacccttca ccaaatggcg tattgagtcg ggcaggtcat
    13561 tcatccttgc tgccacatcg tctgatccga tgatctttgc tgccctctcg agaaggcgtg
    13621 agtgcaggta aaagacatca ccagggtaag cctcgcggcc gggcggacgg cgaagcagca
    13681 gtgaaacttc gcggtatgaa acagcctgct tggagaggtc gtcataaatg atcagggcat
    13741 cacggccggt gtcacggaag aactcgccaa ttgcggctcc tgcaaaggga gcatagaact
    13801 gtgctgccgc agagtcggca gcagtggcgc ttacaatgac ggtatagtcc atggcgccat
    13861 attgttcgag ggtggttgct atgtttgcca cggttgatcc cttctgccct attgccacat
    13921 agatgcagta taccggtttg cccttgtcat actcgctctt ctggttgatg atggtgtcaa
    13981 tggcgatagc cgtcttgcct gtctgccggt caccgatgat gagctcgcgc tgaccgcggc
    14041 ctattggtat catggcatcg atggccttga ggccggtctg caccggctgt ttcaccgggt
    14101 gacggaagat gacgcccggc gcctttctct caaaaggcat ctcgtaaagc tctcccgtta
    14161 ccgggccgcg gccgtcaatc ggatcaccgg tggtggtgat gaccctgcca agcagaccct
    14221 cacccaccat gatggatgat atcctgccgg tgcggcgggc aatgtcaccc tccttgatct
    14281 tctccgaggg gccgagaata acagctccga cattgtcctc ctcaaggttg agaacaatac
    14341 ccttgatgtt tgactcgacg aactcgatca gctcgttgga tcccacattc ttcaggccgt
    14401 aaatgcgtgc aatgccgtca ccgacctgca acaccgtccc cacttcctca acatcaatgg
    14461 ctgtctttac tccttcaagc tgctgcctta aaatctgtga tacctctgaa ggtttaatat
    14521 tagtcatatc tctgaatctt ataaactact tttcggcata tcctgccagt gaaaactcct
    14581 ttctgaatct gttcagcctg ttccttacgc tggcgtcaat gtatgagtca tcgaccctga
    14641 gaataaatcc gccgataatt gactcatcta tctgctcgtt gaactcaatt gtgcctccct
    14701 tttcacctgc gatcagggca gccatctcct tctttgtcgc atcatcgacg ggaacggcag
    14761 tggtgacggt cacctggcgt atgcccctgt gccggcgggt gaggtcaatg aagttacggg
    14821 ctgccgcagc gagataatca ccccggccgt ggctgaacat caaaccgatg aaatcaaccg
    14881 tcagcttctc agcatcatca ccggcaaggg ctatgattgc atcctttctc ttctgctgcg
    14941 ggataacggg atttctgatc acacctttca cctcctccat ggcacagagg cggcttaatg
    15001 ccttcatgtc cctgtagacg ccgtcaacag tgccgccttc aagggctagg tcgaagagcg
    15061 ccttcgcata tcttacagct atcctggctg aattcatggg atcagttcct gtttgcttcg
    15121 atgtcgttaa tgtatctttc aatcagcttc tgctgctcag tatcgctctt aagggtctct
    15181 cccaccactt ttgaggccac ctcgatggcc agcctggcca cctccttctt aacctcggca
    15241 atggccgaca ccttctcacg ctcgatgagt tccctggctc ggcgcaccag tgcatctgac
    15301 tctgactgcc ccttctccct ggcttcggcc atcatccggt cataggccac acgggcatca
    15361 cgaaggatct tgtccctctc ctcgcgcgcc ttgcgcagga tgatctcatt gtcagcctgc
    15421 agcaccttca tctcctcacg ggccttctcg gctgactcaa gcgcgttgtt gatcatgtct
    15481 tcacgcgcct tgacggcttt catgatgggc ccccatgcaa acttccagag caggacgagc
    15541 aggatgagga atataagcgt tgtccagaag actgtcccta tggcgggact ggcaagactg
    15601 ctttgtagta acattttatg ctgtttttaa aaattcattt gtaaagcctg caaccaaccg
    15661 ttgcaggctt tttttgattt tagcccttta tcattgcgac aacgatagcg aagagggctg
    15721 ccccttcgat aagcgctgcc gagatgatca ttgcgagctg aatcttcgag taagcttcag
    15781 gctgacgtgc gatggcatcc atggccgagc cgccgatgcg gccgatacca agtcctgctc
    15841 cgataactgc aagccctgca cctactgctg ctaatgacat tactgcttgt gcttcttcca
    15901 taatattgaa tttaaaataa caaattgata tgttgacttc taatgatgct ccggttcggc
    15961 tgtggccatc ccgatgaaga gggccgacag aagggtgaat acgtatgcct gcaggaaagc
    16021 caccagcaac tcaatgcaat ccatgaagag cacgaagaag atggaaacag gggccacaag
    16081 caccgttttg aaaatgaata tcagcgtcac aaggctcagc acaataatat gtcctgcagt
    16141 gatattcgca aagagacgga tcataagtgc aaaaggtttc gaaaaaagcc ctatgatctc
    16201 gatgggtatc atgatgggca gcagccagaa gggaacgccg ggagcagcaa agacatgcct
    16261 ccagtgcgac ttgttggcgc tgaactgcgt tatcaggaag gtgaaaagcg ccagcacaaa
    16321 ggtcacattg acattgccgg tgacattggc gccaaacgga aagatcggaa ccagcccgag
    16381 taggttgttt ataagtatga aaaagaaaac ggtaagcaag tagggcatga acttcatgta
    16441 cttgtgatcg ccgatgttcg gaatggcaat gtcatccctg ataaacaaaa tcaccggctc
    16501 aagcaccccc tgaagccctt tcggtgcagc agtgcctgct tttttgtagg tcctggcaag
    16561 tgaaagcatc agccagagaa ggataacggc agccaccagc atcccgaaga cagccttggt
    16621 gaccgaaaag tcaaggggag gtttggaacc gtcatggtga ccctcctggt caaccctgat
    16681 gatcttgccc gcatacttgc cctcatgggc tatggcaaaa ccattgtaac tgtgaccatg
    16741 agcaattttc cgcgatgaga agaaatgaac gcctgactcc ctgtcataaa ttattaccgg
    16801 caggtaaatg gctacacttg ttccgtcctt cttcgtcagg atgtgccact cgtgagagtc
    16861 ggcaacgtgc tccatgatga atggagctgc atcaaatacc tcttcgccgc cgtgagcttt
    16921 gccttcctcg tccatggtgc ccgatgaggc tgccggaacc gtacaaacga gcaaaccaag
    16981 caccagaacc agaagatata aatacctgtt tttcaagcta aatttcttta atctgacttc
    17041 tttttcagga caccggtaaa tgtaagaact acatatatgg tgaacgccaa atatagaata
    17101 aaaaataata ttactgaacc tgttgcgtcg tttttaaaga cgagaaagaa aagcagcgca
    17161 agcacaaaag agagcagcat cttcactccc agcgcaatta gtgtcataaa aacacttctt
    17221 tcccggtcag aatggtatcc gttaaaaaaa attaaaagtg aaataaaggc tgcgagggtg
    17281 aagcctgcgg tgagaaatgc ggcagcggaa gtgttgaaga gagaggggta aaaccttccg
    17341 aggactgatt ccatgatgat gaaaaacatg gcgatggctg caaggccgaa gtagaatctt
    17401 actgccaggg gtttaaacat ctcatttgtt tcttaaggct tccttgatta cgagccacat
    17461 agcagccgcc acgcccagca gtgagagcac aaccgtaagg atcggttccg tgccggcgag
    17521 tttgtcaagt ttgactccgc cgagtgatgt gatgaggata atgaccacca tctggatggc
    17581 gatggtgctg tatctggagt aggcgtcaag gccctccctg cctttatttt ccttcttctt
    17641 ttggtctggc tgctgctgtt ccaccgttat caccctcctt catgacgcag ttgcctgtaa
    17701 atacagctcc cggctctata gacagcttgt tggtaatgat ggtgccgttg attttggatg
    17761 atgctttcag gttaaggagc tgtgacacgg ttatcttgcc gtcaatcagg ccggaaacct
    17821 cggagttttt gcattccact tcacccttta ccttaccggt ggggccgatg accacctttc
    17881 ccttggtgtt catgtttccg ttgaggattc cgtctatgcg gatatcgccg gttgatttta
    17941 tgtcacccgt gatctctgtt ccctggctga tgaggttgac tgcagcgccg ttgtctgtct
    18001 ccattatttt tgccatagtg atttatttat tcccgatttc cctgaaatta tttaacacgt
    18061 aaatataaaa acttattgtg ccggatgagg aaaaaaagag ggttcaaaaa gtaacagggt
    18121 gcaataatcc cttccctcat gccgggtcat aggcccacct gagccagatg ctaccccatg
    18181 tgaatcctcc cccgaaagct gccaggatga ggttgtcacc cttcttcagt ttcttctcga
    18241 actcccagag caccaacggt atggtcgcag cggttgtatt gccgtagttt tgtatgttga
    18301 tcataacctt ttcagggggc aggcccattc tccgcccggt ggcgtctata atcctgaggt
    18361 tagcctggtg aggcacgagc caggcaatgt catcggggcc gaggttattc ttttccatga
    18421 tttcggcagc cacatcggcc atgttggaaa cggcgaactt gaacacggcc tgtccttcct
    18481 ggtatatata atgttcccgt cctcccacgg tatcatgaga tgcgggtctc aaagaacctc
    18541 ctgccttcat gtgaaggtat tttcttcctg acccgtcggt tttgaagata taatccatca
    18601 caccgaattc ctcgtatgtg ggttccagga gtactgctcc cgcggcatca ccgaagaggg
    18661 tgcatgtggt ccggtcggta tagtcagtga tagacgacat cttgtcagtg cctaccacta
    18721 tcaccttttt gtacctccct gtttcgacgt aagcggctgc agtctgaagt gcgaaaagga
    18781 atccggagca ggcagcgcca atgtcaaagc tgaatgcgtt tttgatcccg accttgtccg
    18841 aaatgatgtt ggccgttgcc gggaaggcca tgtccggtgt gacggtcgca cagatgagga
    18901 gatcgatctc ttccgggcgt gtgcctgtct tttcaagcag gcccctgacg gcaggttcgc
    18961 ccaggtcaga tgtgcctttc ccttctccct tcaggattct tctttccttg atgccgactc
    19021 gttgcatgat ccactcgtcg gtggtttcaa ccatttcgga taattcctgg ttggtgagcc
    19081 ggtattccgg tgtccaggca ttgatgcctg ttattgctgc ccttatttta gtcatgctcc
    19141 gaatgcttcc ctgatctttc ctgcaagatc tgcatgcacc acatcgcggg tctggagcag
    19201 catgttcatt atggccatgc ggtttgatat tccatgcccg accactacgt tttcatttat
    19261 gccaagaacc ggcgtacccc ccaccacttc gaaattgagc ctgtcaaaaa agctgtcttt
    19321 gatgcctttt gaccttgcaa caacataaaa tttttcagcc attttaagaa ccacgttccc
    19381 cacgaatccg tcgcacacaa tcacgtctgt catctcatcg gtgaagaggt ggtggccttc
    19441 gatattgccc ctgaagttaa catcattggc ttcattcagg agctcatgtg cagctttcac
    19501 tgcggcagat cccttggctt tctccttccc tatgttgaga aggccaaccg tgggttcttt
    19561 tatgccaagc acgtgctttg catatacact gccaagcatc ccgtactgga gcagaacgtc
    19621 aggtttgctg tcaggattga ttccgacatc aagtatgatt gaagatttcc cgttgacgtt
    19681 tggtatgagt gctgcgagcg ccggtctgta aacgcccgga atgacgttca cggtataact
    19741 tgctcccacc atcattgctc cggtgtttcc ggcgctggca aaaccgtcga tgagaccgct
    19801 cttgagcatt ccgaaaccga ctgcgatcga cgaatcgcgc ttctgcgaaa acgccttggc
    19861 cggatggtct gacatctcaa tcacctgtga agtcggtaca atctcaaaaa gagaagcatc
    19921 ggcatcaagc aatgcaagct tctcccttat ccggcgattg tcaccgataa gcacaatccg
    19981 gtctcctgac ggcagctgtc cgagtgcgtc aaatgctccc tctacgacag cattgggagc
    20041 gtaatcacct cccattgcat caagacctat cttcatcgtt cagatttttt gaacggaaag
    20101 ccggtcaggc tgtcgtcttc ttctcaacgg caagctttcc cctgtagtat ccgcactcag
    20161 ggcagaccct gtggtagaga tgggccgatc cgcagttcgg gcatgcagct gttgtaggag
    20221 ccactgcctt gtagtgtgtt ctcctcttgt ctctcctggt actggagtgt ctgtgttttg
    20281 gatttggcat cgcagtaatg aattgagtta attattccct gtcagttttt tcagatcatc
    20341 ccagcgcgga tcggtttctt cctcaccgtg aaccaggaga tcattcagtt tcctgatcat
    20401 gtccggattg caggtgctgt tgccatccgc atcatcagga tgaatgcgct ttatgggtaa
    20461 cgccaggtgg atgtactcat agaaaaactg actcagatct atttcatgtt catccagcgg
    20521 catcgtaatc atgtccggat cagattcatc ccactccctc ccctgcctta tgaaaagcct
    20581 gttggcagtt gagactggca tgtaaaaggc ttcaagacat ctgtcacaca tcacctcggc
    20641 tttcccgttg atgactatgt caagctcgat gtgagtcgaa catttctgaa gcaccaccac
    20701 cgcacgcaac tctcccctct ttatctcaga ctcctcaaat gacccgaaga aatggttccc
    20761 tatctcaaat tcataggtat atctggcctc cttaagtccc gcgagcggta ttgaatacaa
    20821 tccgggcatc tccctttttt tcggtctgca aaattataaa tcctcttcca ataaaaaaaa
    20881 ttatgaacaa aaaagtaaca ccgcctgttc ccttcgctgt tcaccgatgt atatccgccc
    20941 cccgccggaa atgagtggta cggattgaat caacattaat atttttgagg cgtaaatcaa
    21001 tgcaatcacc atgaaagaga aacacggtaa cccgttcgca ggcatagcaa agttcctgat
    21061 atcactggga cttattatga tagttgcaaa acttgacctt ctcgggctgg gagatattcc
    21121 tgactatttc aagtggcaga tgctgctcat cttcttcgga ctgtggtcac tcatcagcct
    21181 cgaacttgtg tcagcagttg tcctcttttc aataggattc tggtttctca tgcctgaaat
    21241 ggccatacaa ctcaccgagc cctacaaaaa gatctactgg ccggcggtga tggtcctctg
    21301 cggagtggca ttcatgctga aaccacttac aaaaagctgc aaataacagt aacatctaat
    21361 aatctcaaac aatggaaaac gaattgaaaa agccggcaag aaacccggaa acctcgaaca
    21421 ggttgatcat aggggtgctg ctgatcatcg ccggactgat cctcatcgtc aagaaatcga
    21481 caatgttgcc ggaacccctc gatcacttca tcgatgacat catcttcagc tggcagatgc
    21541 tgctgatagt gataggcgtc attgccctca ccggatcaga caacaagact cccgggattg
    21601 taatgatctc cgtgggagga ttcttcctga tcccggaact cttcaccgac ttcttcaggt
    21661 cattcaactt cttctggcct gcactcttca tagtgcttgg cgttgtgctc ctggtgaact
    21721 cgaaacggct ggtgaagaag ctcgacttct caacagtcaa caaggcggac tatattgatt
    21781 acgtaaatat cttcagcgga gccgagcggc aactcatcac cgagaacttc cagggaggca
    21841 agatcacttc catcttcgga ggcggtgagg ttgacctgac ccggtcatca atggcacccg
    21901 gtgacaacgt aattgaaatt acctgtatct ttggtggaac aaccatcatt gttcctgaaa
    21961 gctggaacgt ggtgattgag gtcacaccaa tcctcggggg attttctgac agcaggaaga
    22021 tgaggggtga tgtgatcagg gacaatacca ggtcactcac cataaggggt accgtgatat
    22081 tcggaggtgg agagatcaag agttacaaat aatatatgaa gcatccgtta ctggaaaaca
    22141 gaacgaggct ccttgtgtgg tggctggcct ggctgattct tgcagcaggc cagtcactgc
    22201 ttattcattt tggctatgga agccctgccg atgtggcaat tgccgacgga gtgatttcca
    22261 tggttatttt cggcctcctg ggactggctg tatggttccc ggtaagattc ctcctgaaag
    22321 aggataacca ggtatataca acagtggtaa acgtcctgct gaccggttca cttacgattc
    22381 ttgcatggct cttcggaaca aagttcctcg taaggacgct agttaccgaa aaggtagatt
    22441 atgacatttt ctggcactca gtcctggttt tcagagccac ggccggtgtg ctgatcttct
    22501 tcgtgatgtt gcttgtgtac tacctgttcc tgagcgcctc gaggctggct gaaaaggtgg
    22561 cacggcaggc acagcttgaa gcacaggtac gcgaaggcga gctgaagatg ctccggtcac
    22621 agataaatcc tcacttcctg ttcaacagcc tcaactcagt cagctcgctc accgtgacaa
    22681 atccgctgaa ggccagggat atgattgtaa agctgtctga cttcatgcgc tattctctct
    22741 catcaaggaa tgaacagccg gtgaccctcg ggaatgagat ggaaagcctc aggctttacc
    22801 tgcagatcga aaaggtgaga ttcggggaaa ggctcagcat tgaagaagat atcagcccgg
    22861 aatgcctgcc ggccctgatg ccgggaatgc tcctccagcc tctctatgaa aacgccgtta
    22921 aacacggggt ttacgagtcg accgaggagg ttacaatcag gacaaccgtc agaaagcaga
    22981 atgaaatggt gataatcaca gtgacaaaca acgttgacac tgactccgtt gtaaccagaa
    23041 aaggggcagg cataggcctt aaaaatgtca gcagcaggct tgagctgttc tttggcgaca
    23101 aggctgacct cacggtgaat cacggagatg acagtttcac ggtgattgta aggtttccgt
    23161 tccgaaaatc atgattaaca gcataataaa acaatgaaaa caatcagagc gctgatcatt
    23221 gaagacgaag ccccggcaag ggagctgatc agatttatgc tcgccgatca tcctgaggtg
    23281 gagattgtgg gtgaatgcgg agacggattc tgcggggcca gggagataaa ggaaaagaaa
    23341 cccgatctta tcttcctcga tatacagatg cccaagctga ccggcttcga gctcctcgag
    23401 gtgatagatg aaaaaccgga agtgatattc gccaccgcct ttgaccagta tgccatcagg
    23461 gccttcgaac tgaacgcagt ggactatctc ctcaaacctt tttcaaagga gaggtttgat
    23521 gacgcccttg ccaaggcact gggccggctg gagaaagagg cgcctgacca tgagaaaatt
    23581 gaaaaactgg tggctgcagc ctctgaagct gagggatttc tcaccaggat agtcttcaga
    23641 aaaggggcgg gaatcaagat cattcccctg aataatgtct attacctggc ggccgaggat
    23701 gattatgtga tgatctatta cagcgagggg aaagcgctaa aacccaagac catgaagtat
    23761 tatgaggacc atcttcctcc cggcctgttc atgagagttc accggtcata tatcgtgaac
    23821 gtggaacaga tcacccgcct ggaaccctac agcaaggata actatgttgc cgtactcaag
    23881 tcaggggaaa agatcccggt aagccgcgcc ggttacaaga tgctcaggga gaagctctcc
    23941 ttctagtcat ggtccttcag cctgttgcac gcaggagccc tgcgaagcgg tgatccgcct
    24001 ccccggtatc gcctgaatat gaatctcgga accggatcat agccgtgtgt gcctcccggc
    24061 ctgcaacgca gtatcctgcc tgtcgagatc attattcccg taaacggccc gtgcagcctg
    24121 agcgcatcaa tggcatactg tgaacaggtc gggacatgtc ggcatgaggc aggagtccat
    24181 ggcgaaataa accaccggta aaaccatacc ggcagcagga gcaaaaaaga taatatcttt
    24241 gcggcactct tcagcatcgc ctccaaagat aagctgattc ggcatgacaa aatggcctat
    24301 tttcagaact tctttatgat ggcaggcgtt ttgctgaaga aataagaaac ttaaaattgt
    24361 tctttactga tggaaacaat gtttttgcaa aacggtgcat actggataat attcatagta
    24421 ttcatgatac tgagctgggt tgtcagcgcc agactcaagt cgaaattcag aacctattcg
    24481 aaggtgccga tagcatcagg catgacaggc cgtgatgtgg cggaaaaaat gcttcgcgaa
    24541 agcggtgtga cagacgttac cgttcacagt gtggccggtg aactgactga tcattataat
    24601 cctcttaaaa agaccattaa cctcagccgc gatgtttact ccggaaccag cgttgcagct
    24661 gctgccgtgg cagcccatga aacaggccac gcccttcagc atgcacagtc ttacgcatgg
    24721 ctggggatga ggtcaaaact tgccccggtg gtaagcttcg cctccaactg gatacagtgg
    24781 gtgctcattg ccggacttgt tctgctcaac tccaggcccg agatcatgct gataggaata
    24841 gtgctttttg ccattaccac actgttcagc tttatcaccc tcccggttga gatcaacgcg
    24901 tcgaagaggg cactggcatg gctttcaaat gcaggcataa cctactcggg aacagatgac
    24961 aaggctcagg atgctctcaa atgggctgcc tatacgtatg taatagcggc tctcggctca
    25021 ctcgccaccc tgatctatta cattatgctt ttcctgggag caagggacag gtagaagtcc
    25081 cgtttcgcgc agctccgcga ggtcacctgc cttccacgta caggaacttc gttcttttta
    25141 caaggtacct gatgacagcc cagaagataa gggctgcaac cagcagtgtg atgtacatcg
    25201 gctccaggta aatcctgccg gtgacagcaa tgttccggca gagatcgagg aaagcaaaaa
    25261 caagaaaaag gtttgtaatg ctgttgtact cacgcttgag cacgttcctg aatgagaatg
    25321 gcaggtcaga ctttatgtaa tgcctgaacg aaggaaggac agccttcacc cggaaagccc
    25381 atgtgtcata tgtttccccg aactttcggc gcaggtattg ctcctcggca aagatgatac
    25441 gctcataata aagccagtag gccagaacga aaacaatcac ccaccacact gatctcagga
    25501 aaagcaccgg gccgagccac atcaggaagt tgccggcata caggggatgg cggattaccg
    25561 agtaaatacc ctttgtgttg aggtcgtcag ccaactggtc aatcacattc cggcccgagg
    25621 tgttgcgggg aacatatccg accgtgtagg agcgcagcag ttcaccggcc accgagacaa
    25681 ggatacagac tatctcccag gtatagttga aataaattac gcttcggttt gcaatgaaga
    25741 gatagacagc agccggaaca agaattgttg caggcagcca gcttcttctc ctgaataacc
    25801 agttaccgga tttctcaagt tcatggacaa gtgacattct tacggtgttt ggccgtaaaa
    25861 gtatgaaatt ttcagaaact ataccttatc cgaatgaacc cgaggtttat ccgggttttc
    25921 tcttcaccga aagtgctgtt gaagtgctgc cagagtattg agaagtcaac gttctccgcc
    25981 agcgagaagt ccagggaagg gcctgcatag aaaccgtcga ggtcaggaaa ccagatagct
    26041 gccagtgaca gattcaccag cggaagcggc acaaaggtca cctgcgcagc ggcgctgaat
    26101 tcagaaaatg caagctgcct ggctgtcagg cctccgccat agaggtccgt gaaaccgctc
    26161 aggtctgcag gatggcttga atacataacc tgcgcaagcg cggttacctt gtcggaaaat
    26221 gccttgtcaa ttcccagggt ggtgataaaa ttgctctttt cgccgcctgt gccgtcaaaa
    26281 ggggcaaata aggttgcctc tcctcttaag gatactgatc cgatggcacc tgaccatcct
    26341 gttccggcgg tgaaatattc atcatcagtc accccggcaa gaaactgaat atcagtgttc
    26401 agtacgctga aacgaccgag ggcggcagcg gtgatcctgt tgtttccgtc gaccttcacc
    26461 gccgcctcca ctgcggagga ggggccggtt gaccagataa accgcagggc atcgctgccc
    26521 ggcctctcaa catagtcgaa gtcaaaaaat gaataggtgt tgaacagatc attgggattc
    26581 catataaggg cctggctcca gttgatccgc tgccgcccgg ccctgatctg cagctgccct
    26641 gcggtgaaat cgacataagc cctgtcaatc attgtgttaa ggacatatga actgcccgag
    26701 gagatattcc atgagaggtc cgcccagccc gcgtcggcag acagtccgtc aatatatgac
    26761 gggtcaaggc tcaccatgtc accggtaacg aaccggtttc ttatctccag tgcaaaagtt
    26821 aacctgctgc cggcataaac cttgaaattg agccggttat gaagcatgcc cgtgttgatc
    26881 caattctctg atggtttgtc aaatatggcc gtgtgaagcc acgacgcgta ccctccggcg
    26941 gtgaggcggt cagttttccc ggaaccggct ccgggatcat cactgacggc ctcctgtgaa
    27001 taaactgcag ccgcaactga cgtcaccaaa atcattgtaa gaaaaatccg tttcatgcca
    27061 tacagttcat cggtgtcagg gttattccct gtggtttgtc ctctcatccg ataccacttt
    27121 cccgtcctca agggttatga ccctccttgc cctcttcatc acccttgcgt catgtgtgga
    27181 aaagataaac gtcatctcct cctcgcgatt aagtttttcc attatgtcaa ggagagtttc
    27241 cgctgatttt gaatcgaggt ttgccgtggg ttcgtcagcg aggatgaact cggggcgggg
    27301 tgcgagggca cgggccacgg ccaccctctg ctgctggccg ccggagagct gagagggcct
    27361 gctgttgtgc ctgtcagcga gtcccaccgc ctccagcagt tccccggttc gcgccatgcg
    27421 cttctccttc ggccacttct ggaggctcat gatgaatccg acgttttccg cggcagtgag
    27481 gaccggaatc aggttgtacg actgaaagac aaacccgacg tgctccatgc ggaagtctat
    27541 aagctccgaa ggcttcagct ccgaaagcct gtgccccgca acaatgatct cccctgtggt
    27601 gggctggtca agtcctccga taaggttaag cagggtagtc tttccggaac cggaagggcc
    27661 aacgattacg gcaaaatcac cctttgtgaa actgagtgtg acgccgttaa ccgctcttac
    27721 ttcaacctcc gtatcatggt aggttttgta tacatcattg atctcgacta cgttcattgc
    27781 tatgattatt aattcgttat tctcctctta acgcctctac cggattaagc tgcagcgctt
    27841 tgcgtgccgg atataccgcc gagagaatcc cggtaataac gatcagcacg gtaagtatta
    27901 tgaaaaagtc agctccgatt gtcgggtaaa gatgggccga gtatccgaag gcttccagtc
    27961 cctcggaata cttcaccagg ttgatgcctg tgtggttggt tattccgatt acaattccgc
    28021 tgacagccat gccggtaaat cctcccacta cagacaggaa gactgattcg agcattatca
    28081 tcaggaagat tcgcctgcgg ttcattccca ctgcggagag catgccgagc tcgcgggtgc
    28141 gctccaggac agccatcagc attgtgttga caattccgaa agaaagggcg gcaaggataa
    28201 gaaccatgaa gattgcataa atctggttga tatagtcggc tatcatggca aggtctacct
    28261 gtatctcctt ccagttcatc acctccagcc ccggaagagc ctccttcagc acaggcgtca
    28321 cccggtcagt catgtcattc tcttccagcc gggcgatgaa acggtgatat acaccgtcct
    28381 caaggcctgt cagtctgcgc agctcctcca tggggacgaa gactgacgtt gcctcaaaca
    28441 aatcgttatt cgtgcggaat atgccgcata tccggtatgc cgcaccggtc tggttgccgt
    28501 tcacatccag aaatgagagg atgaccctgg aaccttcgcg gtatgaccag gcagcctcac
    28561 ggattgcaag gccgtacttc ttgatttcct tcggggtgag cagcctgtaa taggcctccg
    28621 tcagcttctt ttccgtagtg aaccgctgac cttcaagcgg tttcagcctg tctgtgacgg
    28681 ctgagggtac tcccttatcc accagggctg taagtgattc tgccgtaagg atatacctga
    28741 tgatctgaag ctcccgtgcc agcttttcac caatgaaggc actgttcagc ttcgtgtcgg
    28801 cgccaaaata acttccggtt ccgggaatca gtttcccgga gagggtgaac atctgcttct
    28861 ccttttcaat gtcaatgccg gtgatttcca ccccggcgct gccagaaggg gcggaagcaa
    28921 tacctctgac aattattctg ccggttacat cagtcacccc gggtgtttta cggagggttt
    28981 ccgcaacctt atcaggctcg gatataatat tcaggatgtc gctgctgctg cggaattcct
    29041 tagtgtttac ctggatatgg gagagctcct cgttgactgc ggagtcaatc ctctgaacaa
    29101 tggagctgtt tatcgcagcc agggcgaaga cgctggcaaa tacgcccact gtcaccgcgg
    29161 ctattacggt caggctcctc gtcctgttcc tccagacgtt cttccaggct attgatgtga
    29221 tcatgctgtg tgcagttagg ctctgagagc gttgattaca ttcagtttaa agattttccg
    29281 gatgctgaag gctatggcaa cggcaaggat aaggagaact accaccacct gccagaggta
    29341 gtatgtgtca ggcagaagca tcggcatgac cggctcaaag ccatagtctt cgtacatgcg
    29401 tgccatctcg cccgtgaatc tgagcggcct taaatggccc agaaaaacta tgggtaaaga
    29461 ggcgataacg ccggcaatga ctccgataac tcccatcaac agcatctcca ggccgacaat
    29521 gcctgccagt ctgcccttgc gcatcccgat tgacacaagc attccgaatt cccttctcct
    29581 ttcggccatc atcataagca ctgttccgaa aacaccgaag gcaatgacga ggtagagtat
    29641 cccgatcata atggctccgc tgcggttatc agcttccatc tggtttaaca gaagggcgtt
    29701 catctctttc cagttgcgga ccgcaaggtc ttcgccggta acggcgccaa gcctttgctg
    29761 ggtggcagtg agtaccttat cacttccatc ctttattgct atgatggctg atgtggccat
    29821 gtccggcgcc ccgtacagcc agcgtgcatc atcgagtgtc aggcatacaa acctgttgtc
    29881 aatttccgga ttgggcatgc ttaccacccc ttttacaaca tagagtcctg ccgctgaggt
    29941 accgtgatac ccctgcccga tcaggacgat ggtgtctccc gggacggcct ccaggtagtc
    30001 ggaaagaccg ttcccgataa tggcataacc agactcaccg gcagacagat aactgccggg
    30061 aaatgaggtg tggttccgca gcaggggaag cagtccggca ctgtcctttt tgcttattcc
    30121 aagaactgac atgaggtctt ccgcctcggt gaacgcttct ccggcaaaca gttccgtatt
    30181 ctttgcaagc ctgcccggca gctcttcgcc cttaagcgcc tcgacagctc cgggagtaag
    30241 cttgtaacgc acaatcctgt ttcttatgtt aagagccttg ctctcacgtt cgggttcaac
    30301 ccctatcacc atcactccct gcgtgcggtt tccggctgca gccagtgcaa atgactctat
    30361 acgaggcaca acggcctcaa cgttcggatc ctgctccaat tttctggtca gttcaggatc
    30421 aaggattagc ccgttgtcaa ggccgggttc atcaaggtat gagggatgct gcagctgcag
    30481 gtaaccggag tatgaccgga tgacatcact gtacatcttg tcataggtgc ccagctggaa
    30541 tgatctcatg atcagtgcaa aaaagattgc aaaaaatacc gatgcggcag tgatgagggt
    30601 tctctttctg tttcgccaga gatttctcca ggcgagctga cggtctttca tgacggtaat
    30661 cctttcatta accgggcact atcttatggt tctcatattc tgctgtgaga aaaagctttc
    30721 ttcaacagag atattaaacc tgatctccgt gattttcagg atggtcttat ggccttcctg
    30781 ctctgccgga accaattcga tggttgtcgg aatcagcctg ccgtccatgg tcttaagatc
    30841 catcccgcgt tcagtcctga caagataacc gtcctcatca taaagttcgg ctttaagaac
    30901 gatgaagtcc ttcctgtcca cccaccacat ctggtggctc catagaaccc cggcatcctc
    30961 cctggccgtc agcctgattt tgtggcataa ccggccatca acaacctctt ccccttcaag
    31021 ggaatgatca taatcattta caactgatga ttccctgagt atgtcatcat ttgtatagtc
    31081 tgaccccatc cagccctgcg acatcattga cggaggaagt ttgatcagcc tgctgatggc
    31141 cgggttccag ctccacatct cggtaagccg tttgaggaag gtctgcccct tttccctggc
    31201 cggcgcagtg atgagagtca gggcatagtc atcacccagc gaccatgatt tgaactccac
    31261 cgtcctctgc caggtgggcc gaacgatggt catgctcatt acgctgaaac ctgacttttc
    31321 gccattgaat ttttcgtcag cctttgtaac gatctccttt gccgacagca gttgcgatgc
    31381 tgccgggagg gatactgccg taagcatgat gagaacaaat aatgaagctg cagtctgtcc
    31441 gggtattcct ttcatctgta tttcctgatt aaagtttcga ccattttttc aaaatattca
    31501 tccggatact gtccgggggc aaatatatag gtcaatgcaa atcccttcag tgtgtttgag
    31561 aacatcagca tctcggttac cggatcaaag tcaggccctt cggtttcctt tttccttatg
    31621 aaataatcgg acatcagttt cgccatatag gctgaatatt ccattagcgg gatacctccc
    31681 acggtgaagg ttgcgtccgg atcatcggaa agatgttcat atactcctct ctgaagcatt
    31741 accctgtaaa tcagtttcca gaattgcctg ttctcacgga agatggcaaa gattcttctt
    31801 atgaaatttt cgaattcgtc gggtgtgagg gtactgtcgc ggtcagggtt gaagctgcgg
    31861 tagatctcat cgagcgaccg gttgatgatt gcagccagga gttcctcctt gcttttgaaa
    31921 taattgtaca tcagtccctt ggagatgccg gcatgtttgg ctatgtgact gatggtggtt
    31981 gcatggaacc cgtagtcaga gaagtgctcc agtgccgtat tcataatgag ggcctttttc
    32041 tcctccctca tctcctcgaa ctgtgcagat gatcttggtg acatgatttt tgattaaacg
    32101 ttcattcaac aaaatcccct ttaaaaaacc gtctttgatt gaacggtcgg tcaaagataa
    32161 agcggaattt tatgatttgc aaattttttt tcaaaaatat gccctgcgag gggcaaagca
    32221 ttgcagatgt gcgatttgag atgtgcgatt tgagattgat gatcatccct gtaggcttaa
    32281 agcattgcag atgtgcgatt tgcgatttgc gattgatgat catccccgca ggcataaagc
    32341 attgcagatg tgcgatttga gatttgtgat ttgcgatctt ccccgtaggg gataaaggat
    32401 tgtagcacat gataatctga cctcccccac ctccggcgcc ggcccgaaaa aaatccggga
    32461 gagataatcc ctcccggata agtttattgc agacgcgtca gatctcccga tgttcatttt
    32521 gctctatttc agggcaaaac tgataggaac ggagtagtat atattaaccg gtttaccttt
    32581 cattttgcct ggttcccatt tggggcagag gcgcattacc ctcaccgctt cgttgtctag
    32641 caggggatcg acgtccctga ccacggtgac attctcaaca ttgccttcag aggtgacaat
    32701 aaaccggaga atgaccttcc catgaatacc attgtcttta gcctgtgccg gatattggat
    32761 tgttttatac agaaattcaa acagcgcagc atcaccgccc ggaaacttcg gcatctcatc
    32821 aaccatgata taaacgattc cgttgctgac tgtcccgggc agttcgaacc tgatcagtat
    32881 ttccgaggtg aacggttcat tggcatcttt atttgcaggg cttttccagc ggggtgaaga
    32941 ttccattacc ctggtaagtt cagcggcaaa gagcgggtca gtgtcggatg acggctttac
    33001 ctgtgagacg gagccgtctg tttctactgt ataagtcatc cttgcccatc cgctgatgtt
    33061 tttggctgca gcctcctgcg gataatttgc cttttctgct acccagtcgg caaatgccag
    33121 gtaactctca cccctgaagg tgggaaaatc cgtctcaacc ttgcgcgggt aaggcaagtt
    33181 caggccggac tcctttgcct tgcttctgga gatcacctcg aaagcacctt ttgctccctt
    33241 ttcaccatat tttgcctttg cagcctcagg catcagatag gtatacttgc caaaatcctt
    33301 gcccagcctc ttcgtcacct cttccagaga caagtctgac acaattccgt caataacaac
    33361 aatttcggca cccgatgaag gactacgtgt tgtgaagagc ggattggcct tcatcgtgac
    33421 tgatatcccg cgcgccgatt ctgcagttat ggatacgggc tcatagccac tggctgtgaa
    33481 tatcagagca gcatatccgg gcaggtcact gaaatagaac ttccctgatt cgtcagttgt
    33541 cacgctcagc ggagttcccg acacattgac tgtgacaccc ggtagagggg tgccgtcctc
    33601 tttgaccacg gtcccgtgaa tatcctttat catacctgcc gtctgactgt cacttttgcc
    33661 tgaataggta taaaccgggt cagcaaatgc gtacatcatt agagccgtca gtggcagtac
    33721 aagaagcagt tttagcttcc tgcagggaga ataatttttc tttttcatca tttcaaacct
    33781 gtttgtgtta aatgaaaagt tgaacgaatt ggataggctt atcagcctgg cgctgaatac
    33841 ctggttgaga agcgttgccc tgtaacctgc cggatcagaa gagcgctgca gcgcctcctc
    33901 atcggcaaga tgctcatgat tctgcctgac aagcgccgaa catatccatg caaatggatt
    33961 cacccattgc atcaggctca tcacttcaac aagcaggaga tcgaaccagt gtttctgcct
    34021 gaggtgcacc atctcatggt taaggatctc ccggacctca cgcccgctca gtgaaggatt
    34081 gataaagaca taattgaaaa atgagaaagc ggaggtatat ttttctgttc ttacaagcag
    34141 tgcatcacct tccctgttta ccggtccgat tctgacggtg cggtagactg aaaagaggta
    34201 cttcagcgtc cggaccagca ggaaaagtat tccggcagca tagaataaca tgaaccactt
    34261 cagcggatct gcattttctg taacctccgc tgatgctgcc gccggtgaag gaaaaaccga
    34321 tgccatcccc tgcgtatcct ggatcagcgg ggcaggaaga tcaaccctgt aatgcagtgt
    34381 taccagcgga agaaggattg agagaaccac tcctgtcata aggtagactc tcttaagcat
    34441 gaaaaacctc tccctcctga gaaagaggag gtataacagc gtaaatccag ccagccaggc
    34501 ggccgatttc agcaggtaga gcgcaacctg ttccatatct tattcctgtt gatcatcgtc
    34561 aagctcttca cgggcctcct cgagtatcct gtcgagatcc ttcaccgaaa gattcttctc
    34621 cctggcaaag aacgaagtca ttgccgggaa tgaattgttg aagtacccct cgagcagccc
    34681 gaacagctgg ttctttgaat actcacgctt ggataccagg ggataataca gaaaaacatt
    34741 cgcataggcc cggtggtcca cataaccttt tttctccagc accttcaaca ccgtggaaat
    34801 tgtatttctc gccggcttcg gatcatcaaa tttctccctg atatccttca ccactccctc
    34861 ctccagatcc cataagatct gcattatctg ctcttcagcc ctggtaagtt gcattgtatc
    34921 gtcttttttt taataaaaat tattgctgaa ttaatgttaa caatcgctca tacactttat
    34981 cgggacgctc gcaattctct gaaattatta atccgtcggt atcaataatt gcatagtgag
    35041 gtattcctct tacggagtat ttttcctgaa ggtcagcagc ccagctcccg acacagataa
    35101 ggtttatccc tttgagcttt tcattttcaa ttattgtctc ccatttttcg tattcatcat
    35161 caagacagat attaataatc tctatcggct tgccggaaag gttttctgcc agtttgttca
    35221 gtgaaggtat ctcctcaagg catggtccgc accaggtagc ccagaaatgc aggtaaatca
    35281 atctgccccg gtaatctgac aatgtatgaa aaacccctgc ggaatcttta agataaaaat
    35341 agggggcttc tgagcctggt ttcagtctgt ttccatcttc aagtttttcg atcttcttaa
    35401 gactctgtga ttttctgtca tcgatgtagc tttcctgcag tgatgtcagc ctgaagtctg
    35461 tcgcaatcct gaacgaatcg accagttgaa gttccttcct ggtgtcagcc ccataataca
    35521 aatcatataa aaaatatgca ttgaaatatt caagtatctt tcctttcagg agcgaattga
    35581 tattatgact cgccttactg taatacgcca tcagccgagg cggaccaatc agacggtaat
    35641 caacggaatc agacaataaa aaatagtcgg atataaaccg ccagtataaa ggagacaaga
    35701 cagcctcagg gttataaatc tttacatcgc acggcataag aatgcttccc aggttccggt
    35761 ttttaagata gtagtattga tagaacttca tgttttctga aaaataggag atttcatttt
    35821 cataaagatc acaaaaccat tcaggaagta tatcccagtt tttttcgagg aatttcagcc
    35881 tctcctgcct ggcctggtct atctttttta tcgcatcctc aaactgcctt tctgtttccg
    35941 gttgagtatt gaaagccatg atagcaagcg ggctctgggt ataaaaacag ccaaactcac
    36001 ttctctcatt ttgcaggaaa gcaaatatgg gatcatccat ctgaaaagag atctttctgt
    36061 tgtcatctcc tgcttcgtca agacctaccc tggcaaccag tgtgtcgcca ggcctcatgt
    36121 aagaggtaaa aaccgtatct ctgaactcaa accagaccag ctcaggcctt gtaatactgt
    36181 aacttgcttc gcagactctt ctttcagtca gagtaaatct gtggatcagg cacgattgta
    36241 taggatagta gctgccatga ttgatatcca tcctgacgga atcaaggtca tcctgaacaa
    36301 tgactttgac atacgccact cctccaaaat caatctcagg agagtaatcc tttccatctt
    36361 tacagcttgt cagtaagaca aggactgaaa taaatatcgc cactcctctc attgggaacc
    36421 catagtagta agttgtacaa aagcaacgcc atgcccgttt ccggacactt cttttctatc
    36481 aatccttcac cacaaatata cgactatatt tatagacggg caagtttatt tataattttt
    36541 cgtctatttt tatagtcgta tcgtaaaaag ggattatccc gtaagaagat tttaggtttc
    36601 aatcagcatt tcaatacaaa aatccaaaga gccagagcgg aattatattg cctgatccat
    36661 attcaatatc atcttttaca acaatagcgt tttcagcttt tgctatttgt ttgccggatt
    36721 tcttccttcc ccctacttca actgtgtact taccgtctac aataaagtcc ccttcgtcag
    36781 ggtaattgac tgtgtacctg gcagacacct ggttcaagaa aaaggtttcg cgctcagatc
    36841 ctttgtcggg cttatggagt gtaagcgctt gtgaaagatt ggtattatgc aggtatattt
    36901 tctctggctt cgacataatg ctgatacccc ttgaatctct cctcaactga tatatcagcc
    36961 ctgcctgttc gaggaggtga agatacctgt acaagaggtc acgtgatatt tctaccctgc
    37021 cactgagttc attgatatta ggcgtgaacg gaactgcttc tgcaagaact gacagaagtt
    37081 tcctgatctt aagaatggtg ccatatgtaa tacctgaaac tgcaggaaga tcagagtcca
    37141 gaaccgcact gaccgtattt gagagccgct catggtaata tcggattcct tccaggaaat
    37201 aaggatatgc tccgtatttc agatactcat caaaaatcat cagtggtttt acatatgata
    37261 atacctctct ggctattctt tcgtgatcaa tcaacaattc atctattgat attgcaggaa
    37321 gatcaatatc atatttcatc gagatgaatt ccctcaggga aagttcattc aaatttaagg
    37381 taagcatcct cctgctgaga tctgtttctc ctttctccat ctgcagcgct gaagatcctg
    37441 aaataagcag tttcagattc ggataacggt catacagtga tttgactaat aaagaccagt
    37501 gagaatttct gtgcacttcg tcaataaaaa ggtattctcc ccctctttta acaaaccaat
    37561 cagtaaaatc ctcggccggg tgccgactga aaaaaatgtc atccaggctt ataaacgcag
    37621 ccctggaaga gggaagtcca agagacctca gaaattgaag caacagtgtg gttttgcccg
    37681 tccctcttga gccacgaacc aacacaagtc tctggtcccc gggtatttgg ttcattagat
    37741 atcgggcctg ttttgcgaaa atgtttgtca ctctggactc agagagctca agaattgatt
    37801 ccataattgt tttgataata aaacacaaat atatattaat tgtggttata aaaaaacatt
    37861 atgacatcga tattgatgtt tttaaacaac aataatattc cggggagatt cgctaatatt
    37921 tagaaatgca ggagtgctct cagaacccct cgagcatgtt cagcaacttc tcccgctctg
    37981 tgctgccggg agctgaccgc agaaggtcat tgagccagtt gccgtacatg ggattgggaa
    38041 gcacgatgta tcttgtgccg aagagccggc ggtctgcatc aacagcccca aagccgtggt
    38101 caggaccccg tttctcatag atgccgctga ggtcggccag gttatcacct accagaagta
    38161 aaatctcata atccttttcg ataagcgccc gcctctccac ctttgaggag acggtttcct
    38221 tcagcagcat gtgtgtgctg tcagcattgg caaatcccca cgctgccata tttgaaatgg
    38281 tcggccccat ctcccccacg gttctgttgg atacatagaa aacctcaacg cccagggaat
    38341 cggcgtacct ggtgaactcc accgcacccg gcagcggcct ggcgcgggcg agctccaccc
    38401 acctgaacca gtcgtcattg ttgaatgcct tcttccgtaa aacctgccat ccctggaagg
    38461 gtgagttgtc gagcagggtc tcgtcaatgt caaggaccac tgctgccggt ttttcagatc
    38521 ctggctggcc tgagtctgcc ggtatggaag agtctgccgc gggttttccc tcattatgca
    38581 tttcaaggtt ttccctcagg gcaacctccg cattccggaa gcattggtag aaaagcgccc
    38641 tcatctcggc cgatttctga aaccagagag ttgccaatat caggttgtcc tgatcatcct
    38701 gcgaatcctc ggcggcaagg ttcccggcag acggtgcgga acggtcctcc gccggataag
    38761 atgtctgctt caccgtggca caggaagcca gcattataat gagtgtaaca ataatgagct
    38821 ttttcatccg tttttattct gttgataata aactaattgc cggaaaccgg tccattttgt
    38881 ttgttagtgc ctaattaaca aataattggc cgatgccgtt tatattgttt gaaagatttt
    38941 taactttaac cgtaccatac caaaaagtaa cctgcaatgt caaccttaaa gctgatgctg
    39001 gggatgatcc cctcaacctc caa
//
LOCUS       DAOPXU010000013        38412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_937_length_38412_cov_13.167010, whole genome shotgun sequence.
ACCESSION   DAOPXU010000013 DAOPXU010000000
VERSION     DAOPXU010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 38412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38412
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_937_length_38412_cov_13.167010"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..109)
                     /locus_tag="PKK03_02295"
     CDS             complement(<1..109)
                     /locus_tag="PKK03_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016110865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNX83252.1"
                     /translation="MVSHEIDYRIFGDDIQFVEIELDPNETIVAEAGTMV"
     gene            complement(251..604)
                     /locus_tag="PKK03_02300"
     CDS             complement(251..604)
                     /locus_tag="PKK03_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNX83253.1"
                     /translation="MKINNNAGIFAPEVQMEGASNVRMKILIGPDDGSGNIIMRHFFV
                     APGGNTPFHNHNYEHVIKIERGKGLAVDEKGVEHEVTEGQSLYVRPGDLHQFKNPFGE
                     TFEFLCIIPNPAKLQ"
     gene            729..1502
                     /locus_tag="PKK03_02305"
     CDS             729..1502
                     /locus_tag="PKK03_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNX83254.1"
                     /translation="MKKIIHLSDLHIGYRNCGKNFRTIIDNIAFLKRPASNYIIVITG
                     DLEENANDPLIAEEAMESVARLGSLGYNVLLVPGNHDYGTGSMGNPDCVPVFKERFYK
                     NPGVVFPKLDIIEGVAFIGLDSTAEELHWHDRIFSEGELGDAQLDRLKELLDGGSLDG
                     LKKVVYMHHHPFDFKLWRHLRDSEKLQEVIENRIDMLLFGHYHRNKTTVGRVYHGVWG
                     IPRCYNAGSSTHKNGNTGYHRVIDLSHPDPRMDYDGMFI"
     gene            complement(1559..2278)
                     /locus_tag="PKK03_02310"
     CDS             complement(1559..2278)
                     /locus_tag="PKK03_02310"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HNX83255.1"
                     /translation="MKYLNLILFAGMVVMNYLANALPLNGKTTGQLSDAYPNLFTPAG
                     VTFSIWGFIYILLLIFCVIQFTTSYQAAISRIGWLFGFTCVFNALWIVAWHYERLPLS
                     LILMLGLLVSLIWINVFIRGLPDAFIKAGFGVYLGWICIATIANVSALLVNYGWGGFG
                     ISEQTWTIIMILVGAVIVSLTIWRLENPFIGLAVVWAFAGIMIKRQADNRAIFITAAV
                     MMVAVAVVLMLAFFRRRLSGV"
     gene            complement(2306..3118)
                     /locus_tag="PKK03_02315"
     CDS             complement(2306..3118)
                     /locus_tag="PKK03_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HNX83256.1"
                     /translation="MQSNFKTFFLLAFLTVLLVVAGRLIGGTQGAVIAFGLALIMNVI
                     SFWFSDKIVLKRYRAREVTREDNSRLYSIVESLVQRSGMPMPKVYIIPDSTPNAFATG
                     RNPRHAAVAATQGILTLLNDQELAGVMAHELTHVKNRDTLTSTVAATLVGAITLLGQM
                     GRSGSGSSRNPLVIIGILLLPLAAMLIRMAVSRTREYSADEGGARMSGYPLGLANALN
                     KLGRGVAANPIDHGKPSDSHLFIVNPFTGGLQSLLSTHPPMAERIRRLQEMG"
     gene            complement(3216..3485)
                     /locus_tag="PKK03_02320"
     CDS             complement(3216..3485)
                     /locus_tag="PKK03_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83257.1"
                     /translation="MKELIHIRTECYSGYRDDETPKCFMWQNTRHEVKEVMDRWYQWD
                     PDQKHPVADYFLVETVAGEQYILKHELEHNKWFLCRPGGESVREC"
     gene            complement(3536..6013)
                     /locus_tag="PKK03_02325"
     CDS             complement(3536..6013)
                     /locus_tag="PKK03_02325"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNX83258.1"
                     /translation="MIFAVRIFLAGVAFMASSLPGLSEVPGITDEISTRQYEGTLSLT
                     GLNDTVIVYRDERGMPHIYAKNEHDLYMATGYITAQERLWQMDLVRRSASGRLAEIFG
                     KSFVQADIFSRCLRIREKARQIIDNEDPRILSSLTAYTDGVNAFIASCGKHLPLEFRL
                     LSYTPEPWTLENIASIIGLLGWNLDCRNLTAELYVYQLVSRIGAEKAAALIPDWQATD
                     AVVYPDFALSDSVILRTREMISSLAGIAELGVSGLSSSNNWAVSGNRSETGMPLMSND
                     MHLNLPSPGIWMQMHQVIPGELNVTGVLIPGAPFIIAGHNSSIAWGMTNFRVDAIDLY
                     AEKLNPDNPGLYLLNGDWREMTKMKEVVNIRGGKADTVTICFTHRGPVISGCIDTDDI
                     SPNLQWIGYDYLMGLHGHENPAISMKWSGYDDSDEVKAVWLINRARGWDDFRVGISLF
                     RAISQNCVYADTMGNIGMSSGGGIPLRNGSAILIRNGETDEYDWKGYVPFDQMPWEFN
                     PASGYVSSANNRIVDDDYPWFISNSFELPYRINRIRTMIGEKEKFGIDDFCMMITDQH
                     SDLARLMTPLILRIREDHVPVSPLEAAMLDTLAAWDYDMNPSLVSPTILEYFRTSFRR
                     NLLADELGELYESMWDISAEYYIYRLLAEGYDGMVDDITTERPETQDDIVRRSFTEAI
                     ASLEKDYGRHPEHWNWGRIHSVTFTHPLGSVKILGSLLHLNSRKYPVGGSDHTVCPFF
                     TYKPGFAAVNGASVRHIFNTADWDASLSVIPGGISGVPGSEFYLSQADDYIDGKFYPD
                     HFSREAVLSSVKYTLMLMPPPQLDITH"
     gene            complement(6236..7222)
                     /locus_tag="PKK03_02330"
     CDS             complement(6236..7222)
                     /locus_tag="PKK03_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83259.1"
                     /translation="MKNEETTNRNNASDDPSGISYAENARRDGIARGVRTTAIISLLI
                     LVAAGVVAWLGFSRHQEKQLALMENERGTFNEMLSVRDSTLNDWLQTFTQIENDLNAL
                     KQKENLITIRNSDNVEFTLTRREQILGDIKSISLRLDENRKKLASLNSQLKNSGREIK
                     GLREMIAGLEARIEGHEKEIAALEQSVTEKDLKIGELNTLTADLQNTVTAREEVITTQ
                     QAEMNRAYIASGSYKDLRDLGIVSKDGGFLGIGRTESLVNDIADSLFAQIDITQLKYI
                     PVNSRDAKLVTEHPTDSYQFVREDDGLVASIEIKDPERFWKISRYAVVELKK"
     gene            complement(7393..8745)
                     /locus_tag="PKK03_02335"
     CDS             complement(7393..8745)
                     /locus_tag="PKK03_02335"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNX83260.1"
                     /translation="MAETHLKIFVVEDDEWYNRLLVHTLSLNADYEIRSFNTAAGFLE
                     ALHEAPDIVTLDYRLPDMNGLDLLKKIREENSEVQVILISEQKDIETVVSLLKLGVFD
                     YLVKTNDIRDRLLFTVQNIRNGIGLKREITSLRQEVQNKYMFRNTIMGESPAVRAVFT
                     LMEKALDTNITVILTGETGTGKELAAKAIHYNSKRKDKPFVPVNVAAIPSELIESELF
                     GHEKGAFTGATFRRTGRFEEADGGTLFLDEIGEMNPALQAKLLRALQEKEFVRIGSNK
                     AIKTDCRIIVATNRNLLEEVKKGNFREDLYYRLRGLPIELPPLRERGNDMLILASHFT
                     ERFCAENGIPLRKFTQSAQQKLLGYPFPGNIRELKSVVELAVTLSENQTIETEDIILD
                     PGNPLSEIMNEELTLRQYEIKIIKAMLRKHNNDIKVVAGKLDIGISTIYRLLKEEKQS
                     "
     gene            complement(8738..11101)
                     /locus_tag="PKK03_02340"
     CDS             complement(8738..11101)
                     /locus_tag="PKK03_02340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX83261.1"
                     /translation="MKRFSIQVKIGLLMVLAVALLAATGYLAYKNLSSIVSSINVDVD
                     PGRKLLTIREISMDLQKAENSFRMYSVTNDEKDLQPYYSVISGIDEKVSRLREECSDS
                     PLLITQTDTITKLIEENIYIWNELLFLNQDHRVIEYLIQLPDRLNIRTEGEQEAERGI
                     LRRVFSRSEKNRIDEQALLTDLDRIEKEDQAVREKLKQQELQLAGTSTRIREQFYDLT
                     AKMEYEVSEILSAKAYEADLLSGRTYRWLAMFLISGILLATLVVFTVTRYANRSEAFR
                     VALQRSRDEAENLARTKEQFMANMSHEIRTPVTSITGFTEQLLHEPLDDNSLGKLKII
                     RSSSLHLEKIINDILDFSKLQEGKMVLEEIPFSIGRITEEVHAMFLDQARKNNTVLSF
                     FIDQDTPPVLIGDPYRLKQVMINLVSNSVKFTAGGTVRISVAGIAKQPGEILAVMEFA
                     DTGIGIDEDKISIIFEDFAQEEMSTARRYGGTGLGLSIVRKLVDLMNGKIEFHSRKNE
                     GTTVRCEIPLRVGEPEQLTGETEPVADVPSELGKLRVLIVDDEEYNRMLFKTIFRRWG
                     VECSEAPDGRAALGVLGRERFDLALMDMRMPGLDGAETTGYIRNELKISESEMPVICV
                     SAGFSEEDISRYRKSGINGFLRKPFSEKSLAETIMSVAGHGGDEANSITPGIDLGDLR
                     HIAGGDEKFVREMLAAFLADADKGIAEMAGLLQTGETGRIAEISHKLIPKFRHTGAEN
                     MASLLTRIEQAARDGGDVRQLEILVRSLVKGFPQVRKEITDYLYENG"
     gene            complement(11252..11395)
                     /locus_tag="PKK03_02345"
     CDS             complement(11252..11395)
                     /locus_tag="PKK03_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83262.1"
                     /translation="MCFSAGASFAGSAYLLVTLVPLFISGIKRMWMFGTLMAASCLVT
                     GIL"
     gene            11477..13372
                     /locus_tag="PKK03_02350"
     CDS             11477..13372
                     /locus_tag="PKK03_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNX83263.1"
                     /translation="MNKLAYLFSLCLFISTLFLSGQAGRFSFALVTDTHIGGNTADED
                     LRRTVEDINDIDSIVFVIVSGDVTEFGSDAELRLAKEILDRLEKPWYIIPGNHDTKWS
                     ESGANSFRVVFGGEAFAFSHGGWLFLGTNSGPNMRMGPGQIPRENIVWLDSVLSIPAN
                     RAMKIISVNHYPLDEGLNNWYELTGRLKQLDTRLALCGHGHSNSVLNFEGIPALMGRS
                     NLRGNHPQGGYNIMTITGDTLLMASVRQPGTGEGTEAATGHGAATMPAWASVRLERQD
                     FSADTTRWPRPDYSMNKRYSQVREVWRFQEKADIGAGTLVTGKLAIITSTAGEVKALS
                     LRNGRLKWSFPTGAKIYGTPASDGKRVIAVSADGMVRAFTLKRGRLLWSFDTQQPVVA
                     SPVISGDRVFIAGSSGRCHALDINSGSVLWTNGDINGFVETIPLIYKDMLIFGTWNNR
                     LYALEIATGVTRWVWNNGYSNRMLSPAACVPVAAGDRLFVVAPDRKMACLDVNTGKLL
                     WQSDLGGATVRESMGLSEDRRLVLAKTMDGKVIGVSASGDSPEILWKSDFNIGYDIAP
                     GVITGRDRIIFIPSDKGIVHGASRDNGTLLWSHRISSCLINNIVPLKGNGLIVNSMDG
                     VVVRLGW"
     gene            13604..14188
                     /locus_tag="PKK03_02355"
     CDS             13604..14188
                     /locus_tag="PKK03_02355"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HNX83264.1"
                     /translation="MPGFIDEFLKSYGPEVTKQMSSNFNVDQGTVQKLIPQLAPLILA
                     GLKKQKDTRGGDERVDHILNKYGDSSVLNNIKDLISNKAQAQDVDPNLGGLLGDAGGL
                     QAAQALARKMNIDSSTIMKMIPALAPLVLGALTKKRDTSGKGISGVGALLDADGDGSI
                     LDDVAGFLLKGGSSTQSRGGGLLGSILGGITRRR"
     gene            14200..14745
                     /locus_tag="PKK03_02360"
     CDS             14200..14745
                     /locus_tag="PKK03_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX83265.1"
                     /translation="MKQPVLFLSVLLVLWITGASYWYVCKIRCDCKKAVAPSVEIQAT
                     PDTTAPTPEQLLLASVNEAKHYLSASGAGKGFFPTSSADGDMSGISDEYLKKLKLVLD
                     NNPAARVEVTGHTDITGTAPFNKELGLRRAEFVKSYLVNAGIKAEQIVTSSKGSSEPA
                     ASNSTPEGRAANRRSEIKVII"
     gene            14751..15470
                     /locus_tag="PKK03_02365"
     CDS             14751..15470
                     /locus_tag="PKK03_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83266.1"
                     /translation="MAERFTADLLFILFVLLVAALLGFLIGYLLERGRCRRRIAVLDE
                     EIEGLKAKIRRLEEEKTALDAKLRLAVDEKITLTAKIDGLEGDKTKLREDTRRMDDEI
                     ASLKLTIDRLEKETVALRAAHDEALKAAEARIVPDTPLAAMAHEEPVAPEVFRPDDLE
                     VVIGIGPKIARLLINRGITTWKALAETSVPYIQEILDKDGGERYRIHNPESWPHQARL
                     LDEGRWDEFRELRERLWTEIK"
     gene            15625..17151
                     /locus_tag="PKK03_02370"
     CDS             15625..17151
                     /locus_tag="PKK03_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNX83267.1"
                     /translation="MTFNEDFFYRPRKYNTIELWKNVTPEQWNDAGWQRKNSIRSVDQ
                     LKRVVRLTDHQESEIRRTIGTLRQEGKEPLRITPYYASLMQEDPFNPVFFGEKSQKRL
                     DPIFWQSVPTPANLMFPDAGVEGAMSEGSRSYGAAYQRYPNRVALFVAENTSCASYCV
                     HCQRAKSLDTTADVNSREVDRGLFYIGYNRNINEVLVTGGDALMISFRRLKYILGELS
                     RIPHLRVIRIATRVPVVLPMLITDELLEMIRTASNRYSKGPEKNVYFMTHINHYQEVT
                     ADLAAAVKKITGHGFTIRNQTVLLNHVNDHYRTLAETFRRMFWIGVHPYYLLQCHKEK
                     GIVHFITPIQVGKIYMKHLQGWISGITVPRYAANIEGGGGKVLLMPSGHDTLNLDTNI
                     DDKISESFANVLTWDHKKIVKYEALGRATREEYEMAVGIMDRFIGRKGVFVPRIIIVD
                     EKGNHLETTNRTKLPTFEKTKKAALLDYELNNGDMPLTNPVDAYDEIEAAFLRSGYAS
                     "
     gene            complement(17246..19399)
                     /locus_tag="PKK03_02375"
     CDS             complement(17246..19399)
                     /locus_tag="PKK03_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNX83268.1"
                     /translation="MDISELKARHRLINRWNYKWTPLDKGYTDKTLYINVGTGEMKEK
                     PVPQVMKEKFIGGKGYGLRLLWDATKPDTRWDDPENEIIISSGPVGGITQYSGSGKSL
                     VVTISPQTDSVMDSNVGGFFGPFLKFSGFDAIELQGKAANDVIVFINGVEHYIEVFEA
                     PAEAIDSHVLTEQLTDMFANDESDRKNIGIVSTGSAADNSLIGMLNFTFYDNKRKKIR
                     MKQAGRGGIGTVFRNKKIKAVVCKIPGVKGNLNNVVDLEAIQERGRRFNREMRELDDK
                     QCRMRQVGTAHLMEIMDDHDLLPTHNYKFGSHKDAPKIDSAVWTSFFTQGIPDGCWIG
                     CNMACAKAIDDYEITTGPYAGQKVIVDGPEYETAAGLGSNGGFFDPRYIIETNFYCDT
                     YGICTITWGTSLAFIQECYENGILNKERTGGLELKFGNIPDALELLHRVSRGEGFGLI
                     AGQGIRRMKKIFTEKGWGDPQFMQDIGMENKGLEYSQYMSKESLAQQGGYAMTNKGPQ
                     HDEAWLIFMDMVNNQIPTFENKAEALHYFPMFRTWFGLVGLCKLPWNDVEPETNAQTD
                     EPAKVPEHVDNYVTIYKSVTGREFDKHRLIEDSERVYNFQRVFNIRRGYGRRKDDDQP
                     YRACGPVTVEEYLSRQERYDKQMKEKIGVDPAGMSTEEKIKVTRQFREKQYDLLRDAV
                     YKRRGWNNNGIPTIEHLKKIGMDFPEVIEVVAPLQ"
     gene            19555..19758
                     /gene="thiS"
                     /locus_tag="PKK03_02380"
     CDS             19555..19758
                     /gene="thiS"
                     /locus_tag="PKK03_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNX83269.1"
                     /translation="MKITLNNEPMTIDGDTVTVERLLELRKYTFRLRIVKINGRIIDR
                     DSYAREMIHDGDTVQVIYLMSGG"
     gene            19755..20210
                     /locus_tag="PKK03_02385"
     CDS             19755..20210
                     /locus_tag="PKK03_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83270.1"
                     /translation="MTAQENNDNGKSRKGDFWQRFTLAALIERMPPWMKNKYILTLLI
                     FIVWIILLDPNNLISRVREIKNRSRLQQEKEYYMSRIEEDRSKLNELRTSNENLEKYA
                     REQYRMKRPDEDLFIIVTPEQERKIRRERKVSKTPKPARRQASEKSQKK"
     gene            complement(20211..20816)
                     /locus_tag="PKK03_02390"
     CDS             complement(20211..20816)
                     /locus_tag="PKK03_02390"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNX83271.1"
                     /translation="MNRITVARNTLILTVMVAAVTLMSSGCGKNKPADTDESAVKKQH
                     GGIAYVELDSVIANFDMAKDKSTELEEKTKSSEAELASKSQAFDRDVRDFQNKVQKGL
                     VTRATAAEMEQTLQQQQQTLLARRDEMAYNLNEESLVAQRQVLEYINSYLVEFNADRG
                     YQYILAKQFPGPVLYSDTTLDVTSDVIAGLNAKYSAEKAKK"
     gene            complement(20990..21991)
                     /locus_tag="PKK03_02395"
     CDS             complement(20990..21991)
                     /locus_tag="PKK03_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNX83272.1"
                     /translation="MPLRAKYLTGLLLTFSLFANSQDPQFSQFYANQLYLAPSFAGAT
                     EDYRLALNWRNQWPSIPGVFHTYSFSFDKAVPNFNSGIGFLATYDVAGSGDLSTTNLG
                     LLYSYDIQVNDEWHIRPGVNFKFTYLGLDLTKLVWNSQITTGGTLPPITPPPFDNVAD
                     IDFAVSGLAYNQKTWGGFNIDHLLRPKTSFWGENTSVPIKVDLFGGFQIISRGRLKKK
                     LEEVLSVAANVMFQADFFQSDIGVYYYKNPLVFGVWYRGIPFATSKDVSAQAGDAIIG
                     MLGIKTDQLNIGLSYDFTISNLITSSGGAYEISLIYSFTSSQVKRGGRIHAIPCPEF"
     gene            complement(22125..23423)
                     /locus_tag="PKK03_02400"
     CDS             complement(22125..23423)
                     /locus_tag="PKK03_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX83273.1"
                     /translation="MTEEQKDNGLEDLFRSKLEENEMVAGSDLEGRVMRRLERREFFR
                     FNISRFNFYYLTAAVTALTVAGVMIFSDPGDEKGPSATGVQQETVTGVTGEAVIAGEG
                     EIAIDDSVQASVAPLTETAAATTSAGVSFDRQPDREPALTFGPPAAETVKVSTIASSG
                     ITAASPAALPEIESSVVSGCVPLHVSFACNAEEGHKILWNFGDGGTSSLRNPDYIYDI
                     PGAYTVTLTVTDRKGLSSSAGIVIEVHGRPEAAFEVRKNDPWNEGDRVEFVNLSTEAV
                     DYLWDFGDGIFSTLKDPSHRYADMGTYNVRLVAWSAEGCADSVTVSDLFTDRGMYIRF
                     PNAFVPNEGGPTGGYYNTRTDEESSVFHPVAAGIEDYNLKIYSKAGMLVFESTETELG
                     WDGYHKGDLCAPGVYVWKVRGTYRNGQKFIMAGDVTLLKY"
     gene            complement(23431..23985)
                     /locus_tag="PKK03_02405"
     CDS             complement(23431..23985)
                     /locus_tag="PKK03_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNX83274.1"
                     /translation="MMSEQHIIDGCIRHNRNAQQALWKQYSGYLLGVCMRYATDRPEA
                     EDMLQEAFLKIYFNVSEYSGTGSFRGWLRKVTVNTAITYYHRNLRHKHQVDIDEYVST
                     ETGTAGFEEDFYNAEDLCRVLAELSPGYRMVFNLYTIEGYKHHEIAEMLGIDVNTSKS
                     QYSRARAILRKKLEDLARMKGKFS"
     gene            24166..27390
                     /locus_tag="PKK03_02410"
     CDS             24166..27390
                     /locus_tag="PKK03_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX83275.1"
                     /translation="MTRILQHPILEIPRNEETAFKYNGTFVKGMKGFTIAAALHQSGY
                     PVHTHSLDNRNRSLECGIGKCGACEMLVDGVVRRICITPVDGVREVAEIPAGYHSATA
                     VKANGETRRVYRTTVAIAGAGPTGLAAREMLNKHGVPNLVIDSNDHIGGQFLMQTHQF
                     FFFEKEKQFGGMRGFDIAKNLAGESHAGIMLNSTIWDILEGKRIAAKNMRTGEIFYVD
                     AQYMIVATGAVPFMPAFGNDDLPGVYTAAVIQKMMNQEFTLLGRNILTVGAGNIGYLT
                     SYQLMQAGAKVKAIIEAMPYEGGFPVQANRVRRLGIPVILSHTLVRAIPNSTNDGICG
                     AVIAESRNFTPVPGTEKIIEGIDAINICTGLIPDNQLLIKGREVFGTKVFAAGDAMRI
                     GEGTTAVLRGKQAAMEIIQEMGVRSNYNEYLSLSKEYIDSQQHPTRIIENPWMPDEAR
                     MKARPFVIIDCLYSFACNPCQFACPHGAITKASPDTVPAIDYDKCTGCLACVYQCPGL
                     AIFGYNTAKNQLFLPVEYFVEEGSEVWLVDNDGKKVGEGVIEKVMMKPNRTNVARVRA
                     ITPAGTDIVSARGFIAKENYPVPVTFTPAASMEAKEYVCHCEDVKLEEIMAVIGDRKY
                     ISVDEIKHTTRLGMGPCRGKRCIKRLSMLLRPAGIEIVGDATPRAPLSNQITMGELFP
                     QDTPEIIVTQGDSRKVKVGALVAGGGITGSALMRYLAEAGMKPVLINHDRGSSWRNIA
                     GGRPNFSVPELSDLARHNHELFRELQKTGEINYREIKYVTFAHDETMMSQLEASLAWS
                     DGEMVMPADYAAKIAPGINPEMKKTYRAALLTNDCWQATPGRVIDLIRHLGIKAGGAV
                     EEDSTVTAVSRNGREYTVTVQNHRGEYVEYETPVFINAMGAHGEEFARSLGLYTGTYG
                     VRHQAFITRRLPMMGPGNTPLPMLIDRRNYKGFIAVYGQQLGETGQIIGCASPAADPA
                     EAGRNLKINSKEFMEIVSEVFTSWLPELSTAGFQSLWSGYYTEPRMYIDPEHGLFLGL
                     RGQGFMLGQYLAKLYVDKLTGREVPAYFSRLSLKGDGLPEKAFK"
     gene            27535..28446
                     /locus_tag="PKK03_02415"
     CDS             27535..28446
                     /locus_tag="PKK03_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNX83276.1"
                     /translation="MFNKFIASILPWFPKKFIWIFSRAYISGVTMADAMRVSRELNAQ
                     GVRVTLDVLGEFIKSLDEAEANKQEYLDLIEETQKNGINGNYSLKPTSFGLLLDKEAC
                     YRLMREIVAKAASYNNFCRIDMEDSPCTDLEIELYRRLHAEFPRNVGLVVQAYLKRTC
                     SDLEKMLDMNTPEIPTNYRLCKGIYIEPAEIAYKKYEEINKHYLEDLEFMFRNKIYVG
                     IATHDKPLIEGAYELIRKYNVPKNMYEFQMLYGVTPKLRQSIVDGGHLMRVYVPFGEK
                     WFGYSTRRLKENPKIASHIIKAIFVKG"
     gene            complement(28476..29246)
                     /locus_tag="PKK03_02420"
     CDS             complement(28476..29246)
                     /locus_tag="PKK03_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83277.1"
                     /translation="MITAFTMFRRGRLTRSFCAGKSERSLLLLLVLLTLVLQSVSAQQ
                     HGSILFHGVIFNASTRQPLPDAHWIIRSRSAGATDSRGMFSFYAHYNDTITFTCIGYR
                     HYTLVVTDTLRAREYTAGIYLPGDTTMIPAVVVIPRLGNIRAEIMAERASPNQDVINA
                     TNNLRMAVYQGLANPPELGDPATNYEVLRQKQRMDAYNKGQIASDRMIGLSPLTLIPL
                     IYLAAKGLPEDPEPPGPHISAREMDYLKQVHDSLLIRQ"
     gene            complement(29185..29757)
                     /locus_tag="PKK03_02425"
     CDS             complement(29185..29757)
                     /locus_tag="PKK03_02425"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNX83278.1"
                     /translation="MNFHDLIEKTRTYRRFDGKHEITADTLASLVDLARLSASGANRQ
                     PLKYILVTDPVQRETLYPCLAWAGYLTTWEGPGNGERPTAYIVILGDKEISESFGVDH
                     GIAAQSIMLGATEAGLGGCIIASVKKERLRAAFDIPKQYEVLLVLALGKPVEEVVIET
                     IRNNDVKYWRDDNSVHHVPKRTLDEIILRR"
     gene            complement(29890..30483)
                     /locus_tag="PKK03_02430"
     CDS             complement(29890..30483)
                     /locus_tag="PKK03_02430"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNX83279.1"
                     /translation="MKFELPPLKYEAGDLAPQLSAETLEYHHGKHHLAYVNNLNNLLP
                     GSPFEEATLETMIRKAEGGIFNNAAQVWNHTFYFASLSPTGKRTPSGTVAKAIDAEYG
                     SFEGFREAFTKAATTLFGSGWAWLCMKPDGRLLITQESNAGNPIRSGLMPLMTCDVWE
                     HAYYIDYRNRRPDYIKAFWELLDWKVIGERYDEALKK"
     gene            complement(30545..31375)
                     /locus_tag="PKK03_02435"
     CDS             complement(30545..31375)
                     /locus_tag="PKK03_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HNX83280.1"
                     /translation="MKLTLGFSPCPNDTFIFEAMVHGRVDTEGLEFDWFLADVEELNR
                     RAVEGSVDITKMSFHAYARTAQKYLILDSGSALGRSNGPLVVSRRPVMPDELDDALIA
                     IPGKYTTANLLFSIFWPGATRKREYLFSDIPGAVLSGEADAGLIIHETRFTFRSMGLT
                     MVADTGAMWEKMTGLPVPLGGIVISRNIDPFVASKVERAIRRSIEYARSSPRESVDFI
                     RQHARETDAEVTREHIKLYVNDFSLSLGEEGRRAIEKLFSVALEKGVANPLPASIFLK
                     "
     gene            complement(31372..32040)
                     /locus_tag="PKK03_02440"
     CDS             complement(31372..32040)
                     /locus_tag="PKK03_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83281.1"
                     /translation="MNNASRDIFIAVAHPSEEEAVRIAASNAGLSCEIIVTGVGGAAM
                     SWALQKRFASGDAPSLVIGAGIAGSYVESIRPGTVVTTSSDCFADMGVDDNGRFVPLF
                     GAGLADPDKPPFSGGRIPCSGRWFDLAVGLMPAVRGATVNMASGSQSVIDRIRRAWDP
                     EIETMEGAWLAYTCAMSGIPWISVRAISNMVEPRNMKNWKITMALGRLQEKMTLLLEL
                     IAKL"
     gene            32123..32533
                     /locus_tag="PKK03_02445"
     CDS             32123..32533
                     /locus_tag="PKK03_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNX83282.1"
                     /translation="MIYLTRRERFSAAHRMFRPDLTDEENMIIYGKCSNPLWHGHNYV
                     LKVTVKGEPDNETGYFMNATRLKEIMTDRVIEKLDHKNMNLETDFMAGKVATTENLAM
                     AIWDELEFPLAEEGATLHCVRVEETENNYVEYYG"
     gene            32536..33171
                     /gene="folE"
                     /locus_tag="PKK03_02450"
     CDS             32536..33171
                     /gene="folE"
                     /locus_tag="PKK03_02450"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNX83283.1"
                     /translation="MEKLNDGSGKLSDGYNKIEKWNSEVIEEIAGYYYKILKLLGEDP
                     DREGLLKTPERVAKALSFLTRGYQSDPEEILRSAIFKEEYRQMVIVKDIDIYSMCEHH
                     MLPFHGKAHVAYIPDGYITGLSKVARVVETYAARLQVQERLTTQIRNCIQDNLKPLGV
                     AVVIEAQHMCMQIRGVQKQNSVTTTSAFTGCFLSNLNTREEFMHLIASRLH"
     gene            33204..34088
                     /gene="fabD"
                     /locus_tag="PKK03_02455"
     CDS             33204..34088
                     /gene="fabD"
                     /locus_tag="PKK03_02455"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNX83284.1"
                     /translation="MKAYVFPGQGAQFPGMGKDLYEKFPVAHDMFEKANALLGFRITD
                     IMFSGTDEELRQTRVTQPAIFLHSVVLTAVAGSAFKPAMVAGHSLGEFSALVAAGALS
                     FEEGLALVAGRASAMQKACGINPSTMAAVLGMDDAVVEEVCAALNETVVPANYNSPGQ
                     IVISGTMEGVSAATEELKKRGAKRIVPLSVSGGFHSPCMEPARKELEEAIRKARFNRP
                     LCPVYQNVTGMPVTEPDMIMANLIAQLTSPVRWTQTVQKMVADGATEFIEAGPGNVLQ
                     GLIRKINPNVAASSIDVI"
     gene            complement(34190..37636)
                     /locus_tag="PKK03_02460"
     CDS             complement(34190..37636)
                     /locus_tag="PKK03_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNX83285.1"
                     /translation="MNDCPEHSPGFTRDVHDQQERLKELACVNRATQIIKERKPVDET
                     LTNLVLGLPDALQYPGYTVASIKFQGRAFETPGFTETEWKLEHGFETIAGKGTVTVCY
                     TRQFLPEDEGPFLKEEDELLGRFACLITGYVNSMLAENALSQESRTEPKMPHDHEGRS
                     IRKLLQQFLDRHNAERDVFHDLMPFMVREILLVASLYDAYSIEGEGRFSDYMLGEYYQ
                     MSLTTIPRITGVSGEDEAFSRLEARHYDMIIIMVGVDKSNPMSLCRKIKQKYPYIPTY
                     LLLNNPGDIPFVQEQKSHGVPFDNLFVWTGESRVFFAMAKLLEDKVNVGNDTRKGLTG
                     VILIIEDAPDYYSTYVPMLYSLVLEQTRILIEDVSSDELYKVLKLRARPKILLATTWE
                     EAMAVYARYKDSLLGVISDMRFPRNGELYKMAGLEMLRMIRAEQPNLPTVLQSSDPEN
                     ARFAAELKANFINKNSETLLQDIKSFITYYLGFGHFVYRDTKGRQIAIAKSMKEFEAF
                     LKTVPEDSLVYHAMKNHFSLWLMARGEVKIAKLINPVKISDFQSLREMREFLLDIIRK
                     RRREMNKGKVVNFDESVMVDESNVVSFAGGSLGGKGRGLAFINTLIYSFELGRLLPGI
                     NIRTPFTTIIGTDEFDQFMDTNQLWETANREKDFSALQKQFLAGTLSDDLENKLRVFI
                     RLNTKPLAVRSSSLFEDSLSQPFSGIFGTYLLPNNHPDPEVRLMQLIGAIKLVFSSIY
                     SDNSRIYFEAVNYKIEQEKMAVVIQAVVGNRYGDIFYPHISGTAQSFNFYPVASMTPH
                     DGFAVAAVGLGQYVLDGERAFRFSPAYPALDIVSIRDITRYSQTEFYAVNLARSEVDL
                     LKGENAGLVKLDISRAESDGTLNHAASVYSIDNETLAPGLSMPGPRVVNFANILKYDY
                     VPLANALKTVLEVVREAFGAPVEIEYAVDLNKDEEGRATFYLLQIKPMVGSGAGYSID
                     PESVYTDDMLLVSRKSMGNGLVEGITDIIYVEPERFDNLKTNEMAAEIDAINRQMLAQ
                     GRKYVLIGPGRWGTKDRFLGIPVEWPQISNAKIIVEVSLPNFHIDASQGSHFFHNVTS
                     MNIGYLAVNEATAEGVIMWDRLSKEKEVGKGTWFRHIRFPKPLVIRMDGRLGMAVISL
                     GV"
     gene            complement(37771..>38412)
                     /locus_tag="PKK03_02465"
     CDS             complement(37771..>38412)
                     /locus_tag="PKK03_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNX83286.1"
                     /translation="EIYGLTKGQYSPTSKKGLTTKTSPYGTIEEPFKIGELTIGANGR
                     FFARTVDYNLQLSNEVYKEAAKFHGTAVVEVIQNCVIFNDGTHDAFTSKENRDDNTIT
                     LRHGEKMIFGKNRDKGIILKDLKLRVVKIGENGITEKDILTHDAHEPNPGIHYRLAGM
                     SLPEYPVALGVIRAISAQTYDAAMMEQHERVQARSKIRTVDELLRSGSTWEVK"
BASE COUNT         9153 a         9931 c        10058 g         9270 t
ORIGIN      
        1 ataccatggt gccggcctcg gcaacgattg tttcattcgg gtcaagctct atctcaacga
       61 actggatatc gtcgccgaag atgcgatagt caatttcatg tgataccata agtcagtgtt
      121 ttaatgattc cgtaacgaag ttatgacgat ccccgcatat tacaaaataa gaaaacaggc
      181 aatccatggc tccggaacat gaggatagcc aatcccgcat atgcaatgtc gggaaggcaa
      241 tagtgaagca ctattgcagt tttgccggat tggggatgat gcaaagaaac tcgaaggttt
      301 ctccgaaggg attcttgaac tggtgaaggt cgcccggcct gacataaagg ctctgaccct
      361 ccgtgacctc atgctccacg cccttctcat ccacggcgag gccctttccg cgctctatct
      421 tgattacatg ctcgtaatta tggttgtgaa acggcgtgtt gcctcccggt gcaacaaaga
      481 agtggcgcat gattatgttg cctgatccgt catcgggacc aatcagtatc ttcatcctga
      541 cattgcttgc accttccatc tgcacctccg gtgcgaagat cccggcattg ttgtttatct
      601 tcatgaccct gttgtatttc ctgtactcaa agttacttca gaaacgcctg acacgcgtaa
      661 aagttttgat caaagatgcc gaaatcgcta cctttatggt ttgtacctgt taattgcctc
      721 accctgacat gaagaagatc atccacctct cggacctgca catcggctac cgcaactgcg
      781 ggaagaattt cagaacaatc attgacaaca ttgcattcct gaagcggccg gcctccaact
      841 atatcatcgt aataacgggt gacctcgagg agaacgccaa tgatccgctg attgcggagg
      901 aggcgatgga gtctgtcgcg aggctcggat cactgggcta caatgtgctc ctggtgccgg
      961 ggaaccacga ctatggtacc ggctcaatgg gaaatcctga ctgcgtaccg gtgtttaagg
     1021 agcggttcta caaaaatccc ggtgtggttt tcccgaagct ggacataatt gagggcgtgg
     1081 ccttcatcgg gctcgattcc acggcagagg agctccactg gcacgaccgt atattctctg
     1141 agggggagct tggtgatgcg cagttggaca ggctgaagga gctgcttgat ggtggctcgc
     1201 ttgacgggct gaaaaaggtg gtctatatgc accatcaccc gtttgatttc aagctgtgga
     1261 ggcatcttcg agacagcgaa aagctgcagg aggtgattga gaaccggatt gacatgctgc
     1321 ttttcggcca ttatcaccgc aacaagacca ccgtggggag ggtttatcac ggggtctggg
     1381 gtatcccgag gtgctacaac gccgggagtt caacgcacaa gaacggaaat acgggttatc
     1441 accgggtgat tgatctttcc cacccggatc caaggatgga ttatgacggg atgttcatct
     1501 gagccgggca acaggcaaca gtgaggttcg ccgcaggcga tcaggcattg tagctccgtt
     1561 atacaccgga aagccggcgg cggaagaatg ccagcatcag caccactgca accgcaacca
     1621 tcatcaccgc agcagtgatg aaaattgccc tgttgtcagc ctgccgtttt atcattatcc
     1681 ctgcaaatgc ccatacaacc gccagtccga tgaatggatt ctccagcctc catatggtca
     1741 gcgaaacgat gaccgcgcca accaaaatca ttatgattgt ccaggtctgt tcactgattc
     1801 cgaatccgcc ccacccgtaa ttcaccagca gtgcactgac attggcaatt gtggcaatgc
     1861 atatccatcc caggtagact ccgaaacccg ccttgataaa tgcatcgggc agccccctga
     1921 tgaacacatt gatccatatc agcgatacaa gcagtcccag catcagtatg agggacagcg
     1981 gcagcctttc ataatgccag gccactatcc acagggcatt gaagacacag gtaaagccaa
     2041 agagccatcc tatgcgcgag atggctgcct ggtaagaggt ggtgaactgg atcacgcaaa
     2101 aaatgagaag caggatatag ataaatcccc atatggagaa agtcacaccg gccggtgtga
     2161 agaggttggg gtaggcatcg gagagctgcc cggttgtctt tccgttcagc ggcagggcgt
     2221 tcgccaggta gttcattacc accataccgg caaagaggat aagattaaga tatttcacag
     2281 tcaggagata tttggttaaa catcatcagc ccatttcctg cagccgcctg atccgctccg
     2341 ccattggcgg gtgggtgctc agcaggctct gcagtccgcc cgtgaaggga ttgacaataa
     2401 agagatggct gtcagaaggt ttgccatgat caatggggtt ggcagccaca ccgcggccca
     2461 gcttgttcag ggcattggcc agtcccaacg gatagcctga catcctggcc ccaccctcgt
     2521 cagccgagta ttccctggtg cgtgacactg ccatcctgat cagcatagcg gcgaggggca
     2581 gaagcagtat tccaatgatt accagaggat tacgacttga gcccgagccg ctgcgaccca
     2641 tctggcccag gagggtgatg gcgcctacaa gtgtggctgc aacagtgctg gtgagggtat
     2701 cgcggttctt tacatgggtc agctcgtggg ccatgacacc tgcaagctcc tggtcattca
     2761 gcagggtcaa tatgccctgt gtggccgcca ctgcggcatg gcgcgggttc ctgccggtgg
     2821 caaaggcgtt gggtgtcgaa tcggggatta tatagacctt tggcattggc attcccgacc
     2881 gctgaacaag cgattcaaca attgagtaga gcctggagtt atcctcacgg gtcacctccc
     2941 gtgcgcggta cctcttcagc acaatcttgt cgctgaacca aaagctgatt acgttcatga
     3001 tcagggccag gccaaatgcg atcaccgcac cctgggttcc ccctatcagc ctgcccgcca
     3061 ccacaagcaa tacagtcaga aaagcaagga gaaaaaatgt tttgaaatta ctttgcatga
     3121 ttctacgatt tgagggttag cttttcacct aaaacagtga caacccgaat ttagaaatta
     3181 ttccggtaca ttggttatga tggtacggat aaaaatcaac actccctgac agattctcct
     3241 ccgggcctgc agagaaacca tttgttgtgc tccagttcat gtttcagaat gtactgttca
     3301 cctgcaacgg tttccaccag gaagtaatct gccaccggat gcttctggtc ggggtcccac
     3361 tggtaccagc ggtccattac ttccttcacc tcgtgcctgg tgttctgcca catgaaacac
     3421 ttgggagtct catcatccct gtagcctgag tagcattcgg tccttatgtg aataagctct
     3481 ttcactctgt cgattttttt tagttgaaag ttcctcattc acaaagatag gcatttcagt
     3541 gagtgatgtc aagctggggc ggaggcatga gcatcagggt gtattttacc gaggagagca
     3601 ccgcctcgcg gctgaaatga tcaggataga atttcccgtc tatgtaatca tccgcctggg
     3661 aaaggtaaaa ctcacttccg ggcacacccg agatacctcc cgggatcact gaaagtgacg
     3721 catcccagtc ggctgtgttg aagatatgtc tgactgatgc cccgtttact gcagcaaatc
     3781 ctggtttata ggtaaagaaa gggcagacag tgtgatcact tcccccgacc gggtatttcc
     3841 tggaattcag atgcaggagc gaacccagta tctttacgga acccagcgga tgggtaaagg
     3901 tgacagagtg tatcctcccc cagttccagt gttccggatg gcggccataa tccttctcaa
     3961 gggaagcgat cgcctcggta aagctccggc gtacaatatc atcctgcgtc tcggggcgtt
     4021 ccgtggtgat gtcatccacc atcccgtcat atccttccgc aagcaacctg tagatgtagt
     4081 attcggctga gatatcccac atcgactcat acagctcacc aagctcatcg gccaggaggt
     4141 ttcgcctgaa gctggtccga aagtattcga gaatggttgg tgagacaagc gaagggttca
     4201 tatcataatc ccaggcggcg agggtgtcaa gcattgctgc ctccagcggt gagaccggga
     4261 catgatcttc ccggatcctc aggatcaaag gggtcatcag ccgtgccagg tcagagtgct
     4321 ggtcagtgat catcatgcag aagtcgtcaa tcccaaactt ttccttctcc cctatcattg
     4381 tcctgatgcg gttgatgcgg tatggcagct cgaagctgtt gctgatgaac catggatagt
     4441 catcgtccac aatcctgttg tttgccgagg agacataacc tgaagcggga ttgaattccc
     4501 agggcatctg atcgaacgga acataccctt tccagtcata ttcatcggtt tcgccgtttc
     4561 tgatcagtat tgcgctgccg tttctgaggg gaattccgcc gccgctgctc atcccgatat
     4621 tgcccattgt gtcagcatat acgcaattct ggctaatggc cctgaacagg ctgatcccga
     4681 ccctgaagtc gtcccatccc ctggctcggt taatcagcca gacggctttc acctcatcgc
     4741 tgtcgtcata tccggaccac ttcatgctga ttgccggatt ctcatggcca tgaagtccca
     4801 tcaggtaatc atacccgatc cattgaaggt tcggagagat gtcgtctgtg tcgatgcaac
     4861 cggatatcac cggccctctg tgggtgaaac agatggtaac cgtatctgcc tttcctcccc
     4921 tgatattcac aacttctttc attttagtca tctccctcca atcaccgttt aaaagataca
     4981 ggccgggatt gtcaggattg agcttttcgg catagaggtc gatggcatcg accctgaagt
     5041 tggtcattcc ccatgctatg gagctgttat gccctgctat gatgaaaggt gcgccgggga
     5101 tcaggactcc tgttacattc agttcaccgg ggatgacctg gtgcatctgc atccatatgc
     5161 cgggcgacgg cagattcagg tgcatgtcat ttgacatcag cggcataccg gtctcgcttc
     5221 tgtttccgga tacagcccag ttattgctgg aagacaatcc ggaaacaccg agttctgcaa
     5281 tgccggccag ggaagatatc atctccctgg tacgcagaat gacgctgtca ctcagggcaa
     5341 aatcaggata taccacagca tccgtcgcct gccagtcagg tatcagcgca gcggcctttt
     5401 cggcaccgat cctgcttacc agctgatata catagagttc agcggtaaga ttgcggcagt
     5461 cgagattcca gcccagcaac ccgataatgc tggcaatgtt ttccagagtc catggctccg
     5521 gagtgtatga gagaagtctg aactcgagtg gcaggtgctt tccgcacgag gcgataaaag
     5581 cattcacacc gtcagtatat gctgtcaggg aactgaggat acggggatct tcattatcaa
     5641 tgatttgtct cgccttttcc cggatacgca ggcacctgga gaatatatca gcctgtacaa
     5701 atgacttccc gaagatctcc gcaaggcgtc cggatgcgct cctgcgaaca aggtccatct
     5761 gccacagcct ctcctgtgca gttatgtagc ccgttgccat gtagaggtca tgctcattct
     5821 ttgcataaat atgcggcatg ccccgctcat cacggtatac aatcacggtg tcattaagcc
     5881 ctgtgagaga cagagtccct tcatattgcc gggttgatat ttcatcagtg atcccgggca
     5941 cttcagacag acccgggagg gaagatgcca tgaacgccac tcctgccagg aatattcgta
     6001 cagcaaagat catcccgcga tgcaatattt cactaaaatt aggatatttt tcagctgaaa
     6061 gcaaataata agacgccttt cagcgtcttt ttaggtcccg gaactctccc tcctgctttc
     6121 gccggcagtt gatagtctca gtcccaactt ctgccgttga ttgtcaaccc tgtagttccg
     6181 gggtatatat tagtgtaatg aaggaaattc caaaggaatc accgtcatcc cggctttatt
     6241 tttttagttc tacaacagca tacctcgata tcttccagaa ccgttcagga tccttgatct
     6301 cgatgctggc aacaaggccg tcatcctcac ggacaaactg atacgaatct gtgggatgtt
     6361 cagtcacaag ttttgcatcc ctggaattca caggtatata tttcagctgg gtgatatcaa
     6421 tctgggcaaa caggctgtca gctatatcat tgaccagtga ttctgttctg ccgattccga
     6481 ggaatccccc gtcctttgag acaatcccca aatctctcag gtccttgtag cttcctgagg
     6541 caatgtaagc cctgttcatc tctgcctgct gggtggtgat aacctcttcc ctcgcagtaa
     6601 cggtgttctg caggtcagca gtgagagtat taagttcacc tatctttaaa tctttttcag
     6661 tgacagactg ttcaagagct gctatctcct tctcatgtcc ctcaatcctg gcttcaaggc
     6721 ctgctatcat ttcgcggagg cctttgatct ccctgcccga attcttcagc tgggaattca
     6781 gggatgcaag tttcttcctg ttttcatcga gccgcaggct gatactctta atgtcaccaa
     6841 gaatctgctc tctcctggtc aaagtgaatt cgacattatc agagttgcgg attgtgataa
     6901 ggttctcttt ttgtttgaga gcattgaggt cattttcaat ctgggtgaac gtctgaagcc
     6961 agtcgttcag ggttgagtca cgaactgaca gcatctcatt gaaggtaccc ctctcgtttt
     7021 ccataagtgc cagctgtttt tcctggtgcc tgctgaaccc tagccatgca acgacgcctg
     7081 ccgcaacgag aattaacagg ctgatgattg cggttgttct gactcctctg gcaatgccgt
     7141 ccctgcgcgc gttttccgca taactgatgc ctgaagggtc atcgctggcg ttattacggt
     7201 tagttgtttc ttcatttttc atgtttttcc ttcttattgg ttttccggcc atgaaaaatg
     7261 caaagggtgt gccgggaatg gtggttagct ataatgtatt gataaccaga gcatccacga
     7321 aacatcgcat catgcgaata tgttcaatat gagaactatt ttctcaaaat gagaaaacag
     7381 gattttgaga tgtcatgatt gcttctcttc tttcaggagc ctgtagattg tggagatccc
     7441 gatatcgagt ttgccggcca ccactttgat atcgttatta tgtttcctga gcatggcctt
     7501 tatgatctta atctcatact gtcgcagcgt cagctcctca ttcatgatct cggagagcgg
     7561 atttcccggg tcaaggatta tatcttcagt ttcgatggtt tggttttctg agagggtgac
     7621 cgcaagttca acaactgatt taagctccct gatgtttccg gggaaagggt atccgagcag
     7681 tttttgctgt gcactctgtg taaatttcct gagggggata ccgttttcgg cacagaatct
     7741 ctctgtaaaa tgactggcca ggataagcat gtcattgcct ctctccctca gtggggggag
     7801 ttctatcggg aggccgcgca gcctgtaata gagatcttca cggaagtttc ccttttttac
     7861 ttcctccagg aggtttctgt tggttgccac aattatccgg caatcggttt taatggcctt
     7921 gttgcttcct atccgcacga actccttctc ctgtagggct cttagcagct ttgcctgcag
     7981 tgcgggattc atctccccta tttcatccag gaaaagggtg cctccgtcag cctcttcaaa
     8041 cctgcctgta cgtctgaatg tggcgcctgt gaaagctcct ttctcatgac cgaaaagttc
     8101 actttcaata agttccgaag gtatagctgc tacgttgact ggcacgaatg gtttgtcctt
     8161 ccgctttgag ttgtaatgaa tcgcctttgc tgccagctcc ttcccggttc cggtctcgcc
     8221 cgtaagtatg actgtgatgt tggtgtcaag ggccttctcc ataagggtaa aaacagccct
     8281 gacggccgga ctctcaccca tgatggtgtt gcggaacata tacttgttct gcacttcctg
     8341 gcgaagagag gttatctccc gcttcagacc gatgccgttc ctgatgttct gaacagtaaa
     8401 gagaagccgg tcacggatat catttgtctt taccagatag tcaaagaccc cgagcttaag
     8461 caggctgacc accgtctcga tatccttctg ttcggaaatg aggataacct gcacctcact
     8521 gttctcctcc cttattttct tcaggagatc gaggccgttc atgtcaggca gcctgtaatc
     8581 gagggttaca atgtcaggcg cttcatgcag ggcctccagg aaaccggcag ccgtattaaa
     8641 gcttctgatc tcataatctg cattcaggct gagggtgtga accaggagcc tgttgtacca
     8701 ttcgtcgtct tcaacaacga agatcttcag atgtgtttca gccattttca taaaggtaat
     8761 ccgtgatctc tttccgcacc tgcgggaatc ctttgacaag tgatctgaca aggatttcaa
     8821 gctgccttac atcgcctcca tccctcgcag cttgctcaat acgggtcaga agggaagcca
     8881 tattttcggc gccggtatgg cggaatttag ggataagttt atgagatatt tccgctatcc
     8941 ttccggtctc acctgtttgt aaaagtccgg ccatctcagc gatgcctttg tcagcatctg
     9001 caaggaacgc agcgagcatt tcccttacaa acttttcatc tccgccggca atatggcgga
     9061 ggtcaccgag atcaatcccc ggcgtaatgg aatttgcctc atcgccgcca tggccggcaa
     9121 cactcataat agtctctgcc aggcttttct ccgagaatgg tttccggaga aaaccgttta
     9181 ttcccgattt gcggtaacga cttatatcct cttcggaaaa accggcactc acgcagatca
     9241 caggcatttc tgattcactt atcttcagct cattgcgtat ataccctgta gtttccgccc
     9301 cgtcgaggcc cggcatgcgc atatccatca gagccaggtc aaacctttcc cttccgagta
     9361 ccccgagggc cgccctccca tccggagctt cggagcattc aacaccccat cgcctgaaaa
     9421 tggttttgaa aagcatccgg ttgtactcct cgtcatcaac aataagcacc ctgagcttgc
     9481 caagctcaga gggtacatct gcaaccggct cagtctcccc ggtcaattgc tccggttcgc
     9541 caaccctgag cggaatctcg cacctcaccg ttgttccttc attcttcctg ctgtggaact
     9601 caatttttcc gttcatcagg tcaacgagct tcctgacgat tgagaggcca agtcctgtcc
     9661 cgccgtatct ccgcgcggta ctcatctctt cctgggcaaa atcctcaaag atgatgctga
     9721 tcttatcctc atctatccca atcccggtat cggcaaactc catcactgcc aatatttctc
     9781 cgggttgttt cgcaataccg gcaactgaaa tcctgaccgt gccgccagcc gtaaacttta
     9841 cagagttgct gaccaggttg atcataacct gcttcagccg gtagggatca ccaattaaaa
     9901 ccgggggggt atcctgatca atgaaaaacg acaggacagt gttgtttttc ctggcctggt
     9961 caaggaacat ggcatgcacc tcctctgtta tccttccgat gctgaaggga atctcctcca
    10021 gcaccatctt cccttcctgc agttttgaga aatccagtat gtcattgatt atcttttcaa
    10081 ggtggaggga tgacgatctg attatcttca gttttccaag ggagttatca tccagcggtt
    10141 catgaagcag ttgttcggta aaccctgtta tggaggtcac cggagtgcgt atctcatgac
    10201 tcatgttggc catgaactgt tccttggttc ttgccaggtt ttcggcttcg tccctggaac
    10261 gttgcagggc gacacgaaag gcttcggacc tgttggcata cctggtgact gtaaagacca
    10321 ccagggtggc caggagtatg cccgagatca ggaacatggc aagccagcgg taggtccttc
    10381 cggaaagaag gtctgcctcg tatgccttgg cactgagtat ttccgatacc tcatattcca
    10441 tcttggcggt gaggtcataa aactgctccc tgatcctggt gctggtgccc gccaactgga
    10501 gttcctgctg tttcaatttc tccctgacag cctgatcctc cttttcaatc ctgtcaaggt
    10561 cagtgaggag agcctgctca tcgatgcggt tcttttcgga gcggctgaag actcgtctta
    10621 gaatccccct ttcagcctcc tgttctccct ccgtcctgat attcagtctg tcaggcagtt
    10681 gaatgaggta ttcaatcacg cggtggtcct ggttcaggaa aagaagctca ttccagatat
    10741 agatattttc ttcgatgagt tttgtaatgg tgtctgtctg agtaatgagg agcggggagt
    10801 cactgcattc ctcccggagt ctgctgactt tttcatcaat cccggaaata actgaataat
    10861 agggctgaag gtctttttca tcattggtga cagagtacat cctgaaactg ttttccgcct
    10921 tctggagatc catggatatc tcgcgtatgg tcaggagctt tctgcccgga tcaacatcaa
    10981 cgttaattga gctgacaata gaagagaggt tcttgtaggc caggtagcca gtggcggcca
    11041 gcaatgcaac cgccagaacc atcagcaagc cgatcttgac ctgtattgag aatctcttca
    11101 cggtggattt attggataca atgaacccta aaactatgaa ataatattgt cagtctgaat
    11161 ttttcctgtc cgggtttgct gaaataatgc agtaaatcac gatgcttata agggctgcaa
    11221 agaagcacca tatcgaggtg aggtactcct tttataatat cccggtcaca aggcatgagg
    11281 cagccatcag ggtcccgaac atccacatcc tctttatgcc ggagatgaat agcggtacaa
    11341 gtgttacaag gaggtaagcg ctgccggcga agcttgcacc tgctgaaaaa cacataaagg
    11401 tacttctttg gaattcagca ccaaattaag aatttcaatg atttcgtggt atattgcttt
    11461 caaaagaaga acgtcgatga acaagcttgc atacctgttt tcactttgtc tctttatcag
    11521 caccctgttt ctcagcgggc aggcaggcag gttcagcttc gccctggtca ctgacaccca
    11581 tataggggga aacaccgcag atgaagacct gcggcggaca gttgaagaca tcaatgacat
    11641 tgactccatt gtctttgtaa tagtatcagg tgacgtgacg gaattcggtt cggatgctga
    11701 gctgcgtctc gcaaaggaaa tcctcgacag gcttgaaaag ccctggtaca tcatccccgg
    11761 caaccatgac accaaatggt ctgagagcgg tgccaacagt ttccgcgtcg tctttggagg
    11821 cgaggccttt gccttcagcc atggcggctg gctttttctc ggtaccaact ccggaccgaa
    11881 catgaggatg ggtccggggc agataccgag ggaaaacatt gtatggcttg attctgttct
    11941 cagcattccg gccaacaggg cgatgaagat tatatcagtc aatcactatc ctcttgacga
    12001 agggctgaac aactggtacg agctgaccgg aaggctgaag cagcttgata cgaggctggc
    12061 cctgtgcggt cacggtcatt cgaacagtgt tctgaatttc gaaggtattc cggctctcat
    12121 gggccgttcc aacctgcgcg ggaaccatcc gcagggagga tacaatatca tgaccatcac
    12181 gggcgacact ctgcttatgg cttcggtcag gcaaccggga acaggtgaag gaacggaggc
    12241 tgcaaccggc cacggtgcag ccacgatgcc tgcctgggcc tcggtaaggc ttgagaggca
    12301 agatttcagc gctgacacca ccagatggcc gaggcctgac tattcaatga ataaacggta
    12361 cagccaggtg cgcgaagtat ggcgtttcca ggaaaaagct gacatcgggg ccggaaccct
    12421 ggttacaggg aagctggcaa tcatcaccag caccgccggt gaggtgaagg cactttccct
    12481 caggaacggg cgtttgaaat ggtccttccc caccggggca aagatttacg gcactcctgc
    12541 ttccgacggg aaaagagtca ttgcagtatc agccgacggc atggtaaggg ccttcaccct
    12601 taagagaggc aggctgctgt ggagttttga cacgcagcaa ccggtggtgg cctccccggt
    12661 catcagcggg gaccgtgttt tcattgcggg ctcgtctggc cgctgtcatg cgcttgacat
    12721 taacagcggt tccgtgctct ggaccaacgg tgacattaac gggttcgttg agaccatacc
    12781 gctgatatac aaggacatgt tgatcttcgg cacatggaac aaccggcttt atgcccttga
    12841 gattgcaaca ggagtcacca ggtgggtatg gaacaacggg tattcaaaca ggatgctctc
    12901 tcctgcggca tgcgtacctg tggctgccgg agaccgtctg ttcgtggtgg cgccagacag
    12961 gaaaatggcc tgtctcgacg tcaataccgg gaaactcctg tggcaatccg acctcggcgg
    13021 agcaaccgtc agggaatcga tggggctctc tgaggacagg agactggtgc tggccaaaac
    13081 gatggacggc aaagtcatcg gtgtcagtgc ttcgggcgac agcccggaga tattatggaa
    13141 atctgatttc aacataggat atgacattgc cccgggagtg atcacagggc gtgacagaat
    13201 cattttcatt ccctctgaca aaggtattgt acatggtgcc tcgcgggata acgggacgct
    13261 gctgtggagc cacaggatct catcatgcct catcaacaac atcgtaccgt tgaaaggcaa
    13321 tggcctgatt gtcaattcca tggacggggt ggtggtcagg ctcggctggt aaggtcggaa
    13381 agccccggct ctttgtcaca agggtctggc tctgtacggt tcgccgaatg gctcatcaat
    13441 ctgaaggtcg ggcttgccga gaggtttagc agggcaaata atggcacgtg ttttgcaaaa
    13501 aaaagatttt gattttagtc gccgagtttt tatctttagt gattaactga tcggattttc
    13561 ttaacgcaaa tatttaacac tttcttaaac aacaacgaaa attatgccag gatttattga
    13621 tgaattcctt aagagctatg gccctgaggt gacaaaacag atgtcaagca acttcaatgt
    13681 tgaccagggc acggttcaga agcttatccc tcaactggcg ccgctgattc ttgcgggtct
    13741 caagaagcaa aaggacacgc gtggcgggga tgagagggta gatcacatcc tcaacaaata
    13801 tggggactcc tccgtgctca acaacattaa agatctgatt tccaacaagg cacaggcaca
    13861 ggatgttgat ccgaacctgg gcgggcttct gggtgatgca ggaggactgc aggctgccca
    13921 ggctctggcc aggaagatga acattgattc ttcgacgatc atgaagatga tcccggcgct
    13981 ggcacccctg gtactgggag cgcttactaa aaaacgtgac accagtggca agggaatctc
    14041 aggtgtaggt gcactcctgg atgctgacgg tgacggcagt atccttgacg acgtggcagg
    14101 attcctgctc aagggcggca gctcaaccca gtcaaggggt ggcggcctcc tcggttcaat
    14161 cctcgggggg atcacccgcc gcaggtaaac cgcgtcacca tgaaacagcc ggtacttttc
    14221 ctgtctgttc tcctggtact ctggataacc ggtgcatcat actggtatgt atgcaagata
    14281 aggtgcgact gcaagaaggc agtcgcacct tccgttgaaa tccaggctac cccggatacc
    14341 acggctccga cccctgaaca gcttctcctc gcctccgtaa atgaggcaaa acattatctc
    14401 tctgcatcag gagccgggaa aggattcttc cccacctcat ccgccgacgg agacatgagc
    14461 ggcataagcg atgaatatct gaagaagctg aaactcgtgc ttgacaataa cccggccgcc
    14521 cgtgtggagg tgaccggtca tactgacatc acgggcacgg ctccattcaa caaagagctc
    14581 ggcctcagaa gggctgaatt cgtaaaatct tacctggtca acgcaggcat aaaagcggaa
    14641 cagattgtga catcatcgaa gggttcatca gaacctgcag cgtcaaacag cacgcccgaa
    14701 gggagagccg ctaacagaag aagtgaaatc aaagtaataa tttaaacatc atggcagaac
    14761 gttttacagc agatctgctg ttcattttat ttgtgctgct ggtggcagca ttgcttggtt
    14821 tcctcatcgg ttacctcctt gaaaggggcc gctgcaggag acggatagcc gtactggatg
    14881 aggaaataga agggctgaag gcgaagatac gcaggctgga ggaggagaag acagcgcttg
    14941 acgcgaagct gagactggca gtggatgaaa aaatcaccct cactgcaaag attgacggtc
    15001 tggagggaga caaaacaaaa ctgcgggagg atactcgcag aatggacgac gagatcgcca
    15061 gcctcaaact gacaatagac aggcttgaga aggaaaccgt ggcgctcagg gcagctcacg
    15121 acgaagcact gaaggcagca gaagcccgta ttgtcccgga tacgcccctg gccgccatgg
    15181 ctcacgagga gccggtggca cctgaggtgt tcagacctga cgaccttgag gtagtgatag
    15241 gaatcgggcc aaagattgca aggctgctca tcaaccgcgg gataacaacc tggaaagcac
    15301 tggcagaaac ctctgttcct tacatccagg agatactcga caaagacggc ggtgagcgtt
    15361 accgtatcca caaccctgag agctggcccc accaggcccg cctccttgat gagggccggt
    15421 gggatgagtt ccgtgaattg cgggaacggc tctggaccga aattaaatag aaatttctgc
    15481 ccttcatgca tgtaacgacg ggtctcagac ccgtcgttac gtggtattat gcacaattcc
    15541 tctttaaaaa gaggagaaaa atcctatatt tgttattcag ggtgtggaat accattggta
    15601 ataacataaa aaaaggaggt gctgatgact tttaacgagg atttctttta ccgtccccgg
    15661 aagtacaata ctattgaatt atggaaaaat gtaacgccag aacagtggaa tgacgctgga
    15721 tggcagagaa agaattcaat caggtctgtt gaccagttga aaagggtggt caggctgact
    15781 gatcaccagg aatcagaaat aagaagaact atcggaacgc tgcggcagga aggcaaggaa
    15841 ccgctcagga tcactcctta ctacgcttcg ctgatgcagg aagatccctt taatcctgta
    15901 ttcttcggag agaaatcgca gaaaaggctc gatcccatat tctggcagag cgtccccact
    15961 cctgccaacc tgatgttccc cgacgccggt gtcgagggag ccatgagcga aggatcacgc
    16021 agctacggtg ctgcctatca gcgctacccg aacagggtgg cgctctttgt tgcagagaac
    16081 accagttgcg catcttattg tgttcattgc cagcgggcaa aatcgctcga caccacagcc
    16141 gacgtgaaca gcagggaagt tgacagggga ttgttttaca taggttacaa ccggaacatc
    16201 aacgaggtgc ttgtcaccgg aggtgatgcg ctgatgatca gcttcaggcg gctgaagtac
    16261 attcttggag aactgagccg cataccccac ctgcgggtca ttaggatagc caccagggtg
    16321 ccggtggtgc tgccgatgct cataactgac gagctcctgg agatgatccg gaccgcatcg
    16381 aacaggtaca gtaaggggcc ggaaaagaat gtctacttca tgacccacat caaccattac
    16441 caggaggtaa cggcagacct ggccgccgcc gtgaagaaga tcaccggcca tggttttact
    16501 atccgcaacc agacggtgct gcttaatcat gtcaatgacc actaccggac cctggcagag
    16561 acattcaggc ggatgttctg gatcggagtc cacccctact acctcctaca gtgccacaag
    16621 gaaaagggga tagtccactt tatcaccccc attcaggtgg gaaagatcta catgaagcac
    16681 ctgcagggat ggataagcgg tatcacggta ccgcgttatg cagccaacat cgagggcggt
    16741 ggcggcaagg tgcttctgat gccatcaggg catgacaccc tgaacctcga tacgaacatc
    16801 gatgacaaga tctccgagag ttttgccaat gtgctcacct gggatcacaa gaagatcgtg
    16861 aaatacgagg ctcttggcag ggcaacaagg gaggagtatg agatggctgt cggcataatg
    16921 gaccggttca ttgggcgcaa gggggtgttc gtaccgagga ttataatagt ggatgaaaag
    16981 gggaaccacc tcgagacaac caaccggaca aagttgccaa cctttgagaa aacaaaaaag
    17041 gcggcgttgc tcgattatga gctgaacaac ggggatatgc ccctgaccaa tccggtggat
    17101 gcatatgatg agattgaggc tgccttcctg cggtcaggtt acgcttcata acgtgatgat
    17161 ctgcatcatt tgcaggtagc ggcgggtctc agacccgccg ctacgtcatc gtatgcagaa
    17221 tacacggttg gcagatgaaa ttcgattatt gcaacggggc aaccacctct atcacctccg
    17281 gaaaatccat tccgattttc ttcaggtgct caatggtcgg tataccgttg ttgttccatc
    17341 cgcggcgctt gtagaccgca tcgcgaagga ggtcatactg cttctcgcgg aactgtctcg
    17401 tcacctttat cttttcttca gtgctcatgc ccgcggggtc aacaccaatc ttctccttca
    17461 tctgcttgtc ataacgctcc tgccgtgaga gatactcctc cactgtgacg ggcccgcatg
    17521 cgcgataagg ctgatcatca tccttgcgcc tcccgtaacc acgacgtatg ttaaacactc
    17581 tctggaagtt atataccctc tccgaatcct ctatcagcct gtgcttgtcg aactccctgc
    17641 ctgtcaccga cttgtagatg gtgacgtagt tgtctacgtg ctcgggcacc tttgccggtt
    17701 catcggtctg ggcattggtt tccggctcca catcgttcca tggcagtttg cagaggccca
    17761 ccaggccgaa ccaggtacgg aacatcggga agtagtgaag cgcttcggcc ttgttctcga
    17821 atgtcgggat ctggttgttg accatatcca tgaagatgag ccatgcctcg tcatgctggg
    17881 gccccttgtt tgtcattgcg tatcctccct gctgggcaag cgactccttc gacatgtact
    17941 gtgaatattc gaggcctttg ttttccattc cgatatcctg catgaactgc gggtcgcccc
    18001 atcccttttc ggtaaaaatc tttttcatcc ggcggatacc ctgtccggct atcaggccga
    18061 atccctcgcc gcgtgacacc ctgtgaagaa gttccagtgc atcaggaatg ttcccgaatt
    18121 tcagctcgag gccgccggtg cgctccttgt tcaggatgcc gttctcatag cactcctgga
    18181 tgaatgccag gcttgtgccc caggtgatgg tgcagatacc gtaggtatcg cagtaaaagt
    18241 ttgtttcaat gatatagcgc ggatcaaaga atcctccgtt ggaacccagg ccggcagctg
    18301 tttcatattc cgggccgtca acaatcacct tctgccctgc atagggtccg gtggttatct
    18361 cgtagtcgtc aatagccttc gcacatgcca tgttgcatcc gatccagcag ccgtcgggaa
    18421 tgccctgggt gaagaaggag gtccatactg cggagtcgat cttcggggca tccttatgtg
    18481 atccgaactt gtagttgtgt gtgggcagca gatcatggtc gtccatgatc tccatcaggt
    18541 gtgccgtacc cacctggcgc atgcggcact gcttgtcgtc aagctcgcgc atctcgcggt
    18601 tgaagcgacg cccgcgctcc tggatggctt caaggtcaac cacattgttg agattaccct
    18661 ttacgccggg aatcttgcag acgactgcct tgatcttttt gttgcggaaa acggtgccga
    18721 tgccgccgcg gccggcctgc ttcatcctga tcttcttgcg cttgttgtca tagaaagtga
    18781 agttgagcat gccgatgagc gagttgtcag ctgcagagcc ggtggaaaca atacctatgt
    18841 tcttcctgtc agactcatca ttggcgaaca tatcggtgag ctgctcggta agcacgtgac
    18901 tgtcaatggc ttcggccggc gcctcgaaga cctcgatgta atgctccaca ccattgatga
    18961 aaacaatgac atcattggcg gccttgccct gcagttcgat ggcatcgaaa cccgagaact
    19021 tcaggaaagg tccgaagaag ccacccacgt tgctgtccat caccgagtca gtctgcggtg
    19081 agatggtaac caccagcgat ttaccggagc ctgaatactg ggtgatgccg ccaacagggc
    19141 ctgacgatat gataatttca ttttcggggt catcccatct ggtatccggc ttggtggcgt
    19201 cccacaggag cctgagacca tatccttttc caccgatgaa tttttccttc atcacctgcg
    19261 gaacaggttt ttccttcatc tcgccggtac cgacgttgat gtacagggtt ttgtcggtat
    19321 agcctttatc aagcggagtc catttgtagt tccacctgtt tattagcctg tgtcttgcct
    19381 tgagttcgct gatgtccata ttatgttttt ctattgtttc cgatgataat tacaaatatc
    19441 tggtataaat atccgtaatc taatgataat agtcaatatg cagccgattg ccagacgcac
    19501 taaaattgcg atttgggatt tgcgatttgc gatatgcatg atacctttgc acgcatgaag
    19561 atcaccctta acaatgaacc gatgaccatt gacggcgaca ccgttaccgt cgagagactg
    19621 cttgagctca ggaaatatac attccggctc aggatagtta agatcaacgg gcggattatt
    19681 gaccgcgaca gctatgcccg ggagatgatc catgacggtg atactgtgca ggtgatctat
    19741 cttatgagcg gaggatgacg gcacaggaga ataatgataa cggcaaaagc cggaaaggcg
    19801 atttctggca gcggttcaca ctggcagccc tgatcgaaag gatgccgcca tggatgaaga
    19861 acaagtatat ccttaccctg ctcatcttca tcgtctggat catcctgctt gaccccaaca
    19921 acctgatttc gcgggtacgc gagataaaaa accgcagcag gcttcagcag gagaaggagt
    19981 actacatgag ccgcatcgag gaagaccgca gcaagctcaa tgagctacgg accagcaacg
    20041 agaaccttga gaaatacgcg cgtgaacagt accgcatgaa aaggcctgac gaggatctct
    20101 tcatcatcgt cacacccgag caggaaagga agatcaggcg tgaacggaag gtgtcaaaaa
    20161 caccgaagcc tgctcgcagg caggcttcgg aaaaatcaca gaaaaagtaa ttatttcttc
    20221 gccttttcgg cgctgtattt ggcgttcagg ccggcgatga catcagatgt gacatcaagt
    20281 gtggtgtcgc tgtagagcac cgggccgggg aactgcttgg ccaggatata ctggtatccg
    20341 cggtcagcat tgaactcaac aaggtaagag ttgatatact cgagcacctg cctctgtgcg
    20401 acaagcgatt cctcattgag gttataagcc atctcgtcac gccttgccag cagtgtctgc
    20461 tgctgctgct gcagtgtctg ctccatctcg gcagcagtgg ccctggtaac cagtcccttc
    20521 tgcaccttgt tctggaagtc tctcacatcg cggtcaaaag cctgcgactt ggaggcgagt
    20581 tcagcctcgg agctcttcgt cttctcctca agttcagttg atttgtcctt tgccatatca
    20641 aagttggcaa tcaccgaatc aagttcaaca tatgctattc cgccgtgttg tttcttcact
    20701 gcggactcat cggtgtcggc cggcttgttc ttgccgcatc cggaagacat gagcgtcacc
    20761 gcggccacca tcactgtcaa tatcagggtg ttccttgcaa cagttattct attcatttca
    20821 gaaattaggt tatggttcaa caatttgttg cacaaacata cataaatttt aaatacgaca
    20881 aagagtgtca gaaagtgcag tcgttgatga gctggagccc ggaatcatga aaaccgggaa
    20941 aggatgtgga tgatgctgac cggtgactgt cggtgtgcgt gggccggaat cagaattccg
    21001 ggcaggggat ggcatggatc cttccgccgc gcttaacctg cgatgaggtg aacgaatata
    21061 tcagcgagat ttcatatgcg ccaccggaag atgtgattag gttcgagatt gtaaagtcat
    21121 agctcagccc tatgttcagc tgatcggtct tgatgcccag catgccgatg attgcatcgc
    21181 ccgcctgggc cgacacatcc ttcgaggtgg caaagggaat acccctgtac caaacgccaa
    21241 acaccaaagg attcttgtaa tagtaaacac cgatgtccga ctggaagaag tcagcctgga
    21301 acatcacatt ggccgcaacg gacaaaactt cctcaagttt cttcttcagg cgcccgcggc
    21361 ttatgatctg gaatccgcca aagagatcga cctttatcgg gaccgatgtg ttctcacccc
    21421 agaaagatgt ttttggtctg agcaggtggt caatattgaa tccgccccat gttttctggt
    21481 tataggcaag tcctgacacg gcgaagtcga tgtcggcaac attgtcaaag ggaggcggtg
    21541 tgatgggggg cagcgttcct ccggtggtta tctggctgtt ccacaccagc ttggtgaggt
    21601 cgaggccaag ataggtgaac ttgaaattta ctccgggcct gatatgccat tcatcattca
    21661 cctggatgtc ataggaatag agcagtccca ggtttgtggt gctcaggtcg ccggagccgg
    21721 caacgtcgta agttgcgagg aatccgattc ccgagttgaa attcggtacc gccttgtcga
    21781 atgaaaagct gtatgtgtgg aatactcctg ggatggaagg ccactggttg cgccagttga
    21841 gtgccagacg gtagtcttcc gttgctcctg caaaagacgg cgccaggtat agctggttgg
    21901 catagaactg cgagaactgt gggtcctgac tattcgcgaa aagcgagaag gttagaagca
    21961 gacctgtcag atatttagct cgtaatggca aaccggatcc gattttagtt acacaaagta
    22021 gctaattata cggccaaacc tcaggaaggt tacatcaatt tatatcaaca atctgcccat
    22081 ataatgggca agatattaac aggtcttcca caaccaacta atagtcaata ttttaaaagc
    22141 gtcacatcac ctgccataat aaatttctgt ccgttccggt aagtgccacg gaccttccac
    22201 acatagactc cgggagcgca tagatcgccc ttgtggtagc catcccatcc cagctcagtc
    22261 tcggtgcttt cgaagaccag cattccggcc ttgctgtata tttttagatt gtaatcttcg
    22321 atgcctgcgg cgacagggtg aaacacggaa ctctcctcgt cagtacgtgt gttgtagtaa
    22381 ccgcctgtcg ggcctccctc attgggaacg aatgcgttgg ggaaccttat gtacattccc
    22441 ctgtcggtga agaggtcgct taccgtgaca gaatcggcgc atccttcagc tgaccaggca
    22501 accagacgga cattgtatgt gcccatatcg gcgtacctgt gtgaaggatc tttgagtgtt
    22561 gagaaaatcc cgtcaccgaa gtcccacaga taatcgaccg cctctgtgga cagattcacg
    22621 aactccaccc tgtcaccctc attccacggg tcattttttc gtacctcgaa ggcggcttca
    22681 ggacggccat gcacctctat tacaatgcca gccgaggatg agagtccctt ccggtcggtg
    22741 acggtcagtg tcacagtgta agcgccgggt atgtcataga tgtagtcagg attcctgagt
    22801 gatgaggttc ccccgtcgcc aaagttccat aagattttat gtccttcttc cgcgttgcac
    22861 gcgaagctga catgtagcgg gacgcacccg gagacaaccg atgattcaat ttccggcaac
    22921 gctgccggcg aggctgcagt gatgccgctg ctggcgatag tgcttacttt tacagtctcc
    22981 gctgccggag gtccgaaggt cagtgccggt tcgcggtcgg gctgcctgtc gaaggataca
    23041 ccggctgatg tcgttgctgc agccgtttca gtcagcgggg caactgatgc ctgaacagag
    23101 tcatctatcg caatctcccc ttccccggca ataactgcct caccggtcac cccggtcaca
    23161 gtctcctgct gtacacccgt tgcggatggc cccttctcgt cgccagggtc ggaaaagatc
    23221 atcactcccg caactgtcag tgccgtcacg gctgcggtga gataatagaa attgaatcgt
    23281 gagatattga aacggaagaa ctcgcgccgt tcgaggcggc gcataacacg gccctccagg
    23341 tcactgccgg caaccatctc attctcttcc agcttgctgc ggaagagatc ctcaagaccg
    23401 ttatctttct gttcctctgt catctcctgt tcatgaaaat ttccctttca tccgtgccag
    23461 gtcttcgagc ttcttcctca gaattgccct ggccctgcta tactgggatt ttgacgtatt
    23521 gacgtctatt ccaagcatct ctgcaatctc atggtgctta tagccctcta ttgtgtagag
    23581 gttaaagacc atcctgtatc ccggtgacag ttcagcgagg accctgcaca gatcctcggc
    23641 gttataaaaa tcctcctcga aaccggctgt tcccgtctcg gtgctgacgt actcatcgat
    23701 gtcgacctgg tgcttgtgcc tcaggttcct gtggtagtac gtgattgccg tgtttaccgt
    23761 cacctttctg agccatcccc tgaaagaacc agtgcccgaa tactcactga cgttgaaata
    23821 tatcttcagg aacgcttcct gcaacatatc ctcggcctcg ggccggtcag tggcgtatct
    23881 catgcagacg cccagcaggt atcctgagta ctgtttccac agggcctgct gtgcatttct
    23941 gttgtgcctg atacagccat caatgatatg ttgttcactc atcatcagct gagcactctg
    24001 agaagacaag ggagactgaa aaatggttgc aagcggtgat attttttttc aaaataagga
    24061 aaaaaaatgt attaaatcat ggaatgcttc tgatatccgt cattgaaata agggcagtgt
    24121 cagaactatt tttgggagtt gaattttaat gtgaaagcaa atcctatgac aagaatatta
    24181 cagcacccga tacttgagat tccccgcaac gaggagaccg cctttaaata taacggaact
    24241 tttgtgaagg ggatgaaggg ttttacgata gctgccgcat tgcaccagtc gggttatcct
    24301 gttcatacgc acagccttga caaccgcaac aggagcctgg agtgtggcat aggcaagtgc
    24361 ggagcatgcg agatgcttgt tgacggcgtg gtcaggcgca tatgcatcac cccggttgac
    24421 ggcgtaaggg aagtggccga gatacctgca ggataccatt cagcaacagc ggtgaaggcc
    24481 aacggcgaaa cgcgcagggt ataccgtacc accgtggcaa ttgccggagc cggcccgaca
    24541 gggctggcag ccagggaaat gcttaacaaa catggcgtgc ctaacctggt gatcgacagc
    24601 aacgatcata tcggcggcca attcctgatg cagacacacc agttcttctt cttcgagaag
    24661 gagaagcaat ttggcgggat gaggggattt gacattgcaa agaacctggc gggggaatca
    24721 cacgcgggca tcatgctcaa ctcgacaata tgggatatac ttgaggggaa aaggatagcc
    24781 gcgaagaaca tgcgtaccgg ggagatattt tacgttgacg cacaatatat gatcgtcgcc
    24841 accggcgcag tccccttcat gccggccttc ggcaacgatg acctgccggg ggtctatacc
    24901 gccgcggtga tccagaaaat gatgaaccag gagttcaccc tgctgggcag gaacatactt
    24961 accgtgggtg ccggcaacat cgggtacctc acctcctacc agctgatgca ggccggtgcg
    25021 aaggtcaaag ccatcataga ggcaatgcca tacgaggggg gcttcccggt gcaggccaac
    25081 agggtgagga ggctgggtat tccggtcatc ctgtcgcaca ccctggtcag ggctattccc
    25141 aacagcacca atgacggcat atgcggggca gtgatagccg aaagccgcaa cttcaccccc
    25201 gtcccgggaa cggagaagat catcgaaggc attgatgcca tcaacatatg tacaggcctt
    25261 atccccgaca accagctcct gatcaagggt cgcgaggtgt tcggcacgaa ggtgttcgcc
    25321 gcaggcgacg ccatgcggat cggtgagggt accacggcag tgcttcgcgg taagcaggcc
    25381 gccatggaga tcatccagga gatgggcgtg cgcagtaact acaacgaata tctctcatta
    25441 tcaaaggaat acattgattc acagcagcac ccgacaagga tcattgagaa cccgtggatg
    25501 cctgacgaag caaggatgaa agcgaggccg ttcgtgataa ttgactgcct atacagtttc
    25561 gcctgtaacc cgtgccagtt cgcctgcccc cacggggcga taaccaaggc gtcgcctgac
    25621 acggtgccgg caatagacta tgacaagtgc acggggtgcc tggcatgcgt ttaccagtgc
    25681 cccgggctgg ctatcttcgg atacaatacc gcgaagaacc agctgttcct gcccgtggag
    25741 tatttcgtgg aggagggcag tgaggtctgg ctcgttgaca atgacggtaa gaaagtcggc
    25801 gagggtgtca tcgagaaggt gatgatgaaa cccaacagga caaatgttgc aagggtaaga
    25861 gccattacac cggcaggcac ggacattgtc agcgccaggg gctttatcgc gaaggagaac
    25921 tatcctgtgc ctgtgacctt cacccctgct gccagcatgg aagcaaagga gtatgtttgc
    25981 cactgtgagg atgtaaagct ggaagagata atggcggtca tcggcgacag gaaatacata
    26041 tctgttgacg agatcaagca tacaacgcgt ctcgggatgg gtccctgccg cggcaaacgg
    26101 tgcataaaga ggctctcgat gcttctcaga ccggcgggca tagagattgt aggtgatgcc
    26161 actcccaggg cccctctctc aaaccagatc accatggggg aactcttccc acaggataca
    26221 cctgagataa ttgtcaccca gggtgacagc aggaaggtga aggtgggagc gctggttgcc
    26281 ggcggaggaa taaccggcag cgcccttatg cgttacctgg ccgaggccgg aatgaaaccc
    26341 gttctcatca accatgaccg cggatcatca tggcgcaaca ttgccggggg aagacccaac
    26401 ttcagcgtac ccgaactgtc tgacctggca aggcataacc atgagctgtt cagggagctt
    26461 cagaagaccg gcgagataaa ctacagggaa atcaaatacg tgaccttcgc acatgacgag
    26521 accatgatga gccagctcga ggcctctctg gcctggtcag acggcgaaat ggtgatgccg
    26581 gccgactatg cggcgaagat tgcaccggga atcaacccgg agatgaagaa gacttacagg
    26641 gcggcgctgc tgaccaacga ttgctggcag gcaacgcccg gcagggtcat tgacctgata
    26701 agacacctgg gaatcaaggc aggaggcgct gtagaagagg acagcacagt cacggcagtg
    26761 agccggaacg gccgcgaata caccgtaacc gtgcagaacc accgcggcga atacgtggag
    26821 tatgagactc ccgtcttcat caatgccatg ggcgctcatg gtgaggagtt tgcccgcagc
    26881 ctggggctat ataccggtac ctacggcgtc cggcaccagg ctttcatcac caggcgactg
    26941 ccgatgatgg gaccgggcaa taccccgctt cctatgctca tagaccgtcg taactacaaa
    27001 ggattcatag cagtatacgg acaacagctg ggtgaaaccg gacagattat tggatgtgca
    27061 tcgcctgcag ccgatcctgc cgaagcggga agaaacctga agataaacag caaagagttc
    27121 atggagatcg tgtcagaggt attcacctca tggcttcccg agctctcaac agcaggattc
    27181 cagtcgctct ggagcggtta ctacactgag ccgcgcatgt acatcgatcc tgaacacggt
    27241 ctcttcctcg ggctgcgcgg gcagggattc atgctgggac agtacctggc gaaactgtat
    27301 gtagataagc tgacggggcg tgaggtgcct gcatacttca gcaggctgtc actcaagggc
    27361 gacgggctgc cggaaaaggc tttcaagtaa aaggcaccat aagcgccggc gagcggcgga
    27421 aatagtctcc ggggattcct tttctccagc cgcgatccgg agtaatcaga caggttatca
    27481 ggtattaacc tttttcatat ctttgagcgc caaaaaaaat aaaagcactt aaagatgttc
    27541 aacaagttta ttgcctctat cctgccctgg ttcccgaaga aattcatctg gatcttttca
    27601 cgcgcctata tctcaggagt gaccatggct gatgccatgc gggtctcacg tgagctgaac
    27661 gcgcagggtg tcagggtgac cctcgacgta ctgggagaat tcatcaagtc gcttgatgag
    27721 gccgaagcca acaagcagga gtatcttgac ctgatagagg agacccagaa aaacgggata
    27781 aacggcaatt actcgctcaa gccgacaagc ttcgggctgc ttcttgacaa ggaggcatgc
    27841 taccggctga tgcgcgagat agtggcaaag gcagcatcat acaacaactt ctgcaggatt
    27901 gacatggaag actctccctg cactgacctg gagattgagc tttacaggcg tcttcatgca
    27961 gagttcccgc gcaatgtggg actggtggtg caggcatacc tgaagcggac ctgcagtgac
    28021 ctggagaaga tgcttgacat gaacacaccg gagataccca ccaactaccg tctctgcaag
    28081 ggcatctaca tcgagccggc tgagatagcc tacaagaagt acgaggagat caacaaacac
    28141 tatcttgagg atctcgaatt catgttccgg aataagatat atgtcggcat tgccactcac
    28201 gacaagccgc tgattgaggg agcatatgag ctgataagga aatacaatgt tcccaaaaat
    28261 atgtacgagt tccagatgct ttacggggtc accccgaagc tgcgccagag catcgttgac
    28321 ggcggtcacc tcatgagggt ctacgtcccg ttcggggaaa aatggttcgg atattcaacc
    28381 aggaggctga aggaaaatcc gaagatagca agccacatta tcaaggcgat ttttgtaaaa
    28441 ggttgattgt caaactattg cttactgcct gatggttatt gcctgataag cagcgagtca
    28501 tgcacctgct tcaggtaatc catctccctg gcggagatgt gcggaccagg aggttcggga
    28561 tcttccggca ggcctttggc agccagatat attaaaggta tcagtgttag cgggctaagc
    28621 ccgatcatcc ggtcagaagc gatctgaccc ttgttatagg catccatcct ttgtttctgc
    28681 ctgagcacct cgtaatttgt tgcagggtct cccagttcag ggggattggc caggccctgg
    28741 tagacggcca ttctgagatt gttggtggca ttgatcacat cctggttcgg ggatgctcgt
    28801 tcagccataa tctcagcccg gatgttgcct aaccgtggga tgacgaccac agcggggatc
    28861 atggtggtgt cacccggaag gtaaatcccg gcagtatatt ctcttgcccg cagggtgtca
    28921 gtcaccacca atgtgtagtg cctgtagccg atacaggtaa aagttatggt atcattataa
    28981 tgtgcataga atgagaacat gcccctgctg tcggtggcgc cggcggaacg gcttcggatg
    29041 atccagtgtg cgtcaggcag gggctggcgt gtggaggcgt taaagatgac gccatggaag
    29101 agtattgatc cgtgctgctg ggcggatacg ctctgcagaa ccagggtgag gagaacgagg
    29161 agcagcagga gtgaccgttc ggatttacct gcgcagaatg atctcgtcaa gcgtcctctt
    29221 cggaacatgg tgaacgctgt tatcatctct ccagtatttg acatcgttat tgcgtatggt
    29281 ctctatgacc acctcttcta ccggtttgcc gagggccagt acaagcagaa cctcatactg
    29341 cttcgggatg tcgaaggcag ctctcagtct ctccttcttt acggatgcaa tgatgcaacc
    29401 tcccaggcct gcttcggtgg ctccgagcat aatgctctgt gcggcaatgc catggtcaac
    29461 gccaaaggat tctgatatct ccttgtcacc cagaatcaca atgtatgctg ttggcctttc
    29521 tccgttgccg ggcccttccc aggtggtcag atatccggcc catgcgagac atgggtaaag
    29581 ggtttcgcgt tgcacaggat cagtgacaag aatatactta agtggctgac gattggcgcc
    29641 ggatgccgac aggcgggcca ggtctacaag agaagccagg gtatcggcag tgatctcatg
    29701 tttaccgtca aaccgtctgt aggtgcgtgt cttttcaatc aggtcatgga agttcatagt
    29761 cggaattttg cgtaaagata gggaaaaaag aaagcgctga tttcaaaacg aagcagcccc
    29821 gtgccggggc tgccagatat cactttcaac aggctgccgg ataatgaatg gcggcagcct
    29881 gacgggtgtc tatttcttca gtgcttcgtc gtaccgctct cctatcacct tccagtcgag
    29941 cagctcccag aatgccttga tgtagtcagg acgtctgttt ctgtagtcaa tataataggc
    30001 atgttcccac acatcgcacg tcataagcgg cattagtcct gatctgatcg ggtttccggc
    30061 atttgattcc tgagttatca acaggcggcc gtcaggcttc atgcacagcc atgcccaccc
    30121 tgaaccgaag agcgttgtgg cggctttggt gaatgcctcc ctgaaacctt cgaaagagcc
    30181 atattcggca tcgatggctt tcgccacggt tcccgatggt gttcgtttgc ctgtgggcga
    30241 cagtgatgca aaataaaaag tatgattcca tacctgtgcg gcgttattga aaataccgcc
    30301 ttcagccttt cggatcatag tctcaagtgt agcctcctcg aagggggaac cggggagaag
    30361 gttattcagg ttattgacat aggccagatg atgcttgcca tgatgatact caagcgtctc
    30421 tgcactcaac tgcggtgcga ggtcacctgc ttcatacttc aacgggggta gttcaaattt
    30481 cataatccgg acagattttt agaattacct gtaaatatac taaaataggt tgatttttaa
    30541 cctgtcattt cagaaatatg cttgccggga gcgggtttgc aacgcctttt tccagggcca
    30601 cggagaacag cttttcaatg gcccggcgtc cctcctctcc cagtgacagg ctgaaatcgt
    30661 tgacatataa ctttatgtgt tcgcgggtca cctcagcgtc agtctcgcgg gcgtgctgcc
    30721 tgataaagtc aaccgattcc cgcgggctgg accgggcata ttcgatgctt cgccgtatgg
    30781 ccctctccac ttttgatgca acaaaaggat caatgttgcg actgatcact attccgccca
    30841 gcgggaccgg cagcccggtc attttctccc acatggcgcc ggtgtcagct accattgtca
    30901 ggcccatgga cctgaaggtg aagcgtgtct cgtgaatgat cagccctgca tcggcttctc
    30961 cggagagcac cgccccgggg atatccgaaa agaggtattc gcgtttgcgc gtggccccgg
    31021 gccagaagat gctgaagagg aggttagcgg tggtgtattt tccgggaatg gcaatgaggg
    31081 catcgtcaag ctcgtcaggc atgacaggcc tgcggctcac caccagcggg ccgttgctgc
    31141 gtcccagggc gctgccggaa tcgagtatca ggtatttttg cgccgtgcgg gcgtatgcgt
    31201 ggaagctcat tttggtgatg tcaacactgc cctccacagc cctccgatta agttcctcaa
    31261 catcagccag aaaccagtcg aattccagtc cctccgtatc tactctccca tgcaccattg
    31321 cctcgaagat gaaggtgtca ttggggcacg gggaaaaacc aagtgtcagt ttcataattt
    31381 tgcaattagc tcaaggagca gggtcatttt ttcctgcagc ctgccaaggg ccattgtaat
    31441 cttccagttc ttcatgttcc ggggctccac catgtttgat attgccctta cagatatcca
    31501 tggtatccct gacattgcac aggtataagc cagccaggcg ccctccattg tctctatctc
    31561 cgggtcccat gccctgcgga tccgatctat gactgactgt gagcccgagg ccatgttgac
    31621 agttgcgccc ctgacggcag gcatcagccc gacggccaga tcaaaccacc tgccgctgca
    31681 gggtatccgt ccgccgctga agggtggttt gtcaggatcg gcaaggcctg caccaaacag
    31741 gggaacgaac ctgccgttat catccacacc catgtcggca aagcagtcag aagaggtggt
    31801 gacaacagtt cccggccgga ttgactctac gtagctgcct gcaataccgg ctccgatcac
    31861 cagtgatggg gcatcgccgg aggcaaacct cttctgcagc gcccatgaca tagccgcccc
    31921 tcccaccccg gtaacaatta tctcacagct caagccggcg ttcgatgctg cgatcctcac
    31981 cgcctcctcc tcggaggggt gcgccacagc tataaatata tcccttgatg cattgttcat
    32041 aggcagcatt agttggagtg ataaatatat tatttttgaa caaaatagtc cctgatccgg
    32101 tcaaccaatt aatttagcgg atatgatcta cctgacgaga agagagcggt tcagcgccgc
    32161 acacagaatg ttccgccctg acctcaccga cgaggagaac atgattatct acggcaagtg
    32221 ttccaatccc ctgtggcacg ggcataacta cgtcctgaag gtaactgtga agggcgaacc
    32281 cgataatgaa acaggttatt tcatgaatgc caccaggctg aaggagataa tgacggacag
    32341 ggtcatcgaa aagctcgacc ataagaatat gaacctggag acagacttca tggcaggtaa
    32401 ggtagccacc accgagaacc ttgccatggc gatatgggat gagcttgagt ttccgctggc
    32461 agaggagggg gctacgctgc attgcgtgag ggtggaagag acagaaaata attatgttga
    32521 atactacgga taaccatgga aaaactaaat gacggcagcg gaaagctgtc tgacggctac
    32581 aacaagatag agaaatggaa ctctgaggtc atagaggaga tcgcaggata ttactacaag
    32641 atactcaagc tccttggaga agatcctgac agggaagggt tgctcaagac gcccgagcgc
    32701 gttgccaagg cgctcagctt cctgacgcgc gggtaccagt ctgaccccga agagatactg
    32761 cgaagcgcca tcttcaaaga ggagtaccgg cagatggtga ttgtgaaaga catagacatt
    32821 tactcaatgt gcgagcatca tatgctgccg tttcacggca aggctcacgt ggcgtacatt
    32881 cccgacggtt atatcaccgg gctgagcaag gtagccaggg tggttgagac ctacgctgcc
    32941 aggctccagg tgcaggagag gctgacaaca cagatacgaa actgcatcca ggataacctg
    33001 aaacctcttg gtgtggctgt agtcattgag gcacagcata tgtgcatgca gatacgaggt
    33061 gtgcagaagc agaactcggt cactacaacc tcggctttca cgggttgttt cctctcgaac
    33121 ctgaacacgc gcgaggagtt catgcacctg atagccagca ggctgcattg aaattattta
    33181 ttcacacaat acctgaatca attatgaagg cttatgtatt ccccgggcag ggcgcgcagt
    33241 tccccggcat gggaaaggat ctctatgaga aatttcccgt ggcgcatgat atgtttgaaa
    33301 aggccaacgc actgcttggc ttccgcataa ctgacatcat gttttcaggc accgacgagg
    33361 agctgaggca gacccgtgtg acgcagcctg cgatttttct tcactcggtg gtactgacgg
    33421 ctgtagccgg aagtgccttt aaaccggcga tggttgccgg ccactcgctg ggtgagtttt
    33481 cagcactggt tgctgccgga gctctgtcct ttgaggaggg actggcgctt gttgccggaa
    33541 gggcctccgc gatgcagaag gcatgcggca ttaacccgtc gaccatggcc gcagtgctgg
    33601 gcatggatga tgcggtggtg gaagaggtat gtgccgcgct gaacgagacc gtggtgccgg
    33661 ccaactacaa cagtcccggc cagatagtga tctccggcac catggaagga gtgtcagcag
    33721 ctacggagga actgaagaag aggggagcaa aacggatcgt gccgctgtca gtgagcggcg
    33781 gattccattc gccatgcatg gaacctgccc gcaaggagct ggaagaggcc atacgaaagg
    33841 cacgcttcaa ccgcccgctt tgccccgtct accagaatgt gacaggcatg ccggtgacgg
    33901 aacctgatat gattatggcg aacctgattg cacaactgac ctcaccggta aggtggacac
    33961 agaccgtaca gaagatggtt gccgacggtg cgactgaatt cattgaagcg ggaccgggca
    34021 acgtgttgca gggattgatc aggaagataa atccgaacgt tgccgcatca tcgattgacg
    34081 tgatatgatg ttacatgcat ggcaggtatg cgaatcttat tccgtagcgg cgggtctgag
    34141 acccgccgtt acctgcatat aatgtaatta cccggataac aggcaattat catacaccca
    34201 atgatatcac cgccatcccc agcctgccgt ccattcgtat gaccaggggt ttgggaaacc
    34261 tgatgtgcct gaaccaggtg cctttaccaa cttccttctc cttactcagc ctgtcccaca
    34321 ttatgacccc ctctgccgtg gcttcgttca cagccaggta accgatgttc atggaggtga
    34381 cattatggaa gaagtgtgat ccctgcgatg catctatatg gaaattaggc aggcttacct
    34441 ccactatgat ctttgcgttt gagatctgcg gccactcaac aggtatcccg aggaaccggt
    34501 ccttggtgcc ccaccggccc ggaccgatga gcacatattt ccttccctgc gccagcatct
    34561 gccggttgat ggcgtcgatc tcggcagcca tctcattggt tttcaggtta tcaaatcttt
    34621 ccggttccac atagatgata tccgtgattc cctccaccag cccgttgccc atgctctttc
    34681 tggataccag cagcatatca tcggtgtata cactttcagg atcgatgctg tagcctgcac
    34741 cgcttccaac catgggcttt atctgcagga ggtagaaggt ggccctcccc tcctcatcct
    34801 tgttcaggtc aacggcatac tctatctcca ccggtgcacc aaaagcttcc ctcaccacct
    34861 caaggaccgt tttcagtgca ttcgccagcg gtacataatc atacttgagg atatttgcaa
    34921 aattgacgac acgcggtccg ggcatggaaa gccccggcgc aagcgtttca ttatcaatac
    34981 tgtatacaga tgcagcatga ttcagggtgc catcgctttc tgccctcgag atatcaagct
    35041 tgaccagtcc ggcattttcg cctttgagaa ggtcaacctc gctgcgggcc aggttgaccg
    35101 cataaaactc cgtctgcgag tacctggtaa tgtccctgat ggagacaatg tcaagagcag
    35161 ggtatgcggg agagaacctg aaagccctct caccgtcaag cacatactgc cccagaccca
    35221 ctgccgccac ggcgaagccg tcatgcgggg tcatgctggc tacaggatag aagttgaatg
    35281 attgtgccgt gccgctgata tggggataaa agatatctcc gtacctgttt cccacaacag
    35341 cctgaatgac cacagccatc ttttcctgct ctatcttgta gtttacggcc tcgaaataga
    35401 tacgtgaatt gtccgaatag atcgaggaga aaacgagttt gatggccccg atgagctgca
    35461 tcagcctcac ctccggatca gggtggttat tgggcaggag gtaagtacca aagattcctg
    35521 aaaagggctg gctcagcgag tcctcgaaca ggcttgacga tctcaccgcc agtggtttgg
    35581 tattgagcct gatgaatacc ctcagtttat tttcgagatc atcactgaga gtgcccgcca
    35641 ggaactgttt ctgcagggcg ctgaaatcct tttcgcggtt tgccgtttcc catagctggt
    35701 tggtatccat gaactggtcg aactcatccg tgccgataat tgtcgtgaag ggcgtcctga
    35761 tgttaattcc aggcagcagg cgcccgagct caaaactgta aatcagggtg ttgatgaatg
    35821 caaggccacg tccctttcct ccaagtgatc caccggcaaa gctcacaacg tttgactcgt
    35881 caaccatcac tgactcgtcg aagtttacca ccttgccctt attcatctcc cggcgtctct
    35941 tcctgatgat gtcaaggagg aactccctca tctccctgag gctctggaag tcagatatct
    36001 tgaccgggtt tatgagcttg gcaatcttca cctctcctct tgccatcagc cagagggaga
    36061 agtggttttt cattgcatgg taaaccagcg aatcctcagg aacggtcttg aggaatgcct
    36121 caaactcctt catcgacttg gctattgcaa tctgcctgcc ttttgtgtca cggtagacga
    36181 agtggccaaa gccgaggtaa taggtaataa atgatttgat atcctgaagc agtgtttcgg
    36241 agttcttgtt gatgaagttg gctttcagtt ctgctgcaaa gcgggcgttt tcggggtctg
    36301 acgactgcag cacggtgggc aggttaggct gttccgccct gatcatccgg agcatttcga
    36361 gcccggccat cttgtaaagc tcaccgtttc gcgggaaccg catgtcagag atgacgccga
    36421 ggaggctatc cttgtacctg gcatatactg ccatggcttc ctcccatgtg gtggccagca
    36481 gtatcttggg tctggccctg agcttaagca ccttgtaaag ctcatcggag gagacatcct
    36541 cgatgaggat ccgtgtctgc tcaaggacaa gggaatagag catcggcacg taggtggaat
    36601 agtagtcagg ggcgtcctcg attatcagta tcacccctgt aagtcccttc cgggtgtcat
    36661 ttccaacatt gaccttgtct tcgagtagtt tcgccatggc gaagaagaca cgtgactcgc
    36721 ctgtccagac gaagaggttg tcaaacggca ctccgtgact cttctgttcc tggacgaagg
    36781 ggatgtcacc ggggttattg agaagcaggt aagtggggat atacgggtat ttctgcttga
    36841 tcttgcggca gaggctcatc gggttgctct tgtctacccc taccataata atgatcatgt
    36901 cataatggcg cgcctcaagg cgcgaaaagg cttcatcctc tcctgatacg ccggtgatgc
    36961 gcgggatggt tgtgaggctc atctggtagt attcaccgag catgtagtca gagaaccgcc
    37021 cttcgccctc tatggagtat gcatcataaa ggctggcaac cagtagtatc tctctgacca
    37081 tgaagggcat aagatcatga aaaacatccc tttcggcatt gtgcctgtca aggaattgct
    37141 gcaacagttt cctgatcgac ctgccttcat gatcatgagg catcttcggt tcagtcctgc
    37201 tctcctgtga cagggcgttt tcagccagca tgctgttgac ataaccggtg atcaggcagg
    37261 caaacctgcc cagaagctca tcttcttcct tcaggaacgg accttcatcc tcagggagaa
    37321 actgccgcgt ataacatact gtcacagttc ctttgcccgc aatagtctca aagccatgct
    37381 caagtttcca ttccgtttcg gtgaagccgg gagtctcaaa tgcgcggccc tggaatttta
    37441 tgcttgccac cgtgtaaccg ggatactgaa gagcatcggg cagcccgagt accaggttcg
    37501 tgagtgtctc atcaacaggc tttctctcct tgataatctg tgtggcacgg ttaacacaag
    37561 ccagctcctt caggcgttcc tgctggtcat gcacgtcacg ggtgaagccg gggctgtgtt
    37621 cggggcaatc attcatctgg gcaccaggta aagtcggata ccaaaataaa caaaaagaga
    37681 atagaatatt acagccggtc ccctgcatta aggaaccggc tgttttttat caccggggag
    37741 agcctggcaa cggtgccgtt tcagtggtga ttacttcacc tcccaggttg atccgcttct
    37801 gagcagttca tccacggttc tgatctttga tctggcctgt acccgctcat gctgctccat
    37861 catggctgcg tcataggtct gtgcactgat agcccttatc acgccaagcg ccacagggta
    37921 ttcaggaagc gacatgcctg cgagcctgta gtgaatgccc gggttgggct catgggcatc
    37981 gtgggtaagg atatccttct cggtaatacc attctcgcca atctttacca ctctcagctt
    38041 gaggtccttc aggatgatac ccttgtcgcg gttcttgcca aagatcatct tttcgccatg
    38101 gcggagagtg atggtattgt cgtcgcggtt ctccttgctg gtgaaagcgt catgagttcc
    38161 gtcattgaag atgacgcagt tctggatgac ctccacaacg gccgtgccgt ggaactttgc
    38221 cgcctccttg taaacctcgt ttgagagctg caggttatag tcgaccgtgc gggcgaagaa
    38281 gcgtccgttg gcgcctattg tcagttcccc gatcttgaag ggctcttcta ttgtgccata
    38341 tggcgaagtt ttggttgtga ggcccttttt cgaggtggga gaatactgcc ctttggtaag
    38401 gccataaatc tc
//
LOCUS       DAOPXU010000014        38241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_947_length_38241_cov_13.842063, whole genome shotgun sequence.
ACCESSION   DAOPXU010000014 DAOPXU010000000
VERSION     DAOPXU010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 38241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38241
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_947_length_38241_cov_13.842063"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..2687
                     /locus_tag="PKK03_02470"
     CDS             45..2687
                     /locus_tag="PKK03_02470"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNX83287.1"
                     /translation="MKRIAFIAAVLLLCTVAMTAAANQDSIPGKTYRARFTSSPPVID
                     GSIDDAAWQAGEWQGDFTQYEPDNGAAPLQKTEFILLYDDNNIYVAFKAWDTSADSIV
                     RRISRRDNGEGDFVGIGFDSYHDLQTGFAFVTNAAGVKNDFIWSQDGRMEDDTWDPIW
                     FTGAKVYEWGWAAEMRIPLTQLRFRISDDGVWGLEVFRRIFRNQEMSVWQHIDRNASG
                     LVHNFGTLSGLTGIKPRKQADITPFVVGSLETFEKDDGNPFATGTNWKYNGGLDAKFG
                     LTNNMTLDLTLNPDFGQVEADPSEVNLTAYETFFEEKRPFFIEGKNITSFRTGLGDGD
                     LGFDNLFYSRRIGRSPHLGVDTDDDEFARVPRVTPIISAAKLTGKTPDGLSIGIVEAV
                     TAEGRAVIDSLGHRSEQTVEPFTNYLVSRVMKDFGGGRTIIGGAFTNTHRFLDNTGID
                     GLVRNANTAGIDFTRYFGEERDWFVSATAAGSNLTGSKTAIEDLQRSSIHYFQRPDAD
                     YVELDPERTSLNGFGGNVQAGKMGGHWNFMAFTYLKSPGLDLNDVGYLQSADGIISGL
                     WSAYRFDKPFSIFRQIRPNANFWTGWDFGGTHLFTGGNLSLYVQFKNLWWTNFGVNYE
                     GNSLSNNLLRGGPAMLYPSSVNSWFNLGTSDSKKVSASVFANASRGAENVSERYSAGI
                     DFTVKPGQSFTASVEPSYSINRNEIQYVTCTDDDERYILSRIDQQIVSMSVRLNYNIT
                     PDLTVQYWGQPFLAAMDYSRFKAVTDPRAETLAGRYHLISEDEMTYDSDDDMYLVDED
                     YDGITDYSFDNPDNNYDQFLSNLVLRWEFSPGSTLFLVWSQTRNYSDETGSFSLSHNL
                     DNLYTSKKPYDVFLVKFTYRFGLR"
     gene            2825..5470
                     /locus_tag="PKK03_02475"
     CDS             2825..5470
                     /locus_tag="PKK03_02475"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNX83288.1"
                     /translation="MKSGLLILFSCITLVLPAQEYEKKEYTASRTENPPVINGVPDDE
                     EWTEGEWAGDFWQYDPAEGAPVSQKTEFKLLYDDYNIYVAIKAYDTAPDSIVSRMTRR
                     DDTEGDEVSVALDSYFDQRTAFGFTVSAAGVKGDLMWIDDGLTEDDSFDPIWYVKTGI
                     FDWGWAAEMRIPLTQLRFSASDQQVWGLEVLREIYRHNETVMWQPIARNASGLVHNAG
                     LLKGLTNIRPRKLFDLTPYGVARLETYEGEQGNPYHDGTDIKGNIGLDAKIGVTNNMI
                     LSLSLNPDFGQVEADPSEVNLTAFETFFSEKRPFFIEGNNITDYNLGLGSGGRGNDNM
                     FYSRRIGRQPRLSHSAGQGEYAYTPAFTPIIGSAKLTGKSAGGLSVGFIEAVTANVST
                     RIHDPLTGETTFRTAEPLTNYGVGRVQKDFSGGKTIIGGMVTSTIRSLDAETEDYFHK
                     SATSAGIDFTRYFGNMNYIFRLRTAVSNVTGTDEMIAGTQRSVIHNFARPDADYLDYD
                     ITRTSLSGFGGNLMAGKIGGNWQFIYLSSWKSPGFEINDIGYMLMADMHMGVGVINYN
                     IYKPFSVFRSMSFGTNLIHLMDFGGHTNAVAVSQSWTANYQNQWRTFLSGQLTSPETD
                     NHLLRGGPAMKMPGQLYLSASIDSDWRKKLSGEVDFSVTRTFNDVMTSYELSFELEYR
                     PFNTLTLNIEPGWSRTVNMMQYVTFRHLEGLNDQSERYIFGSIDQKIYSISIRADFNI
                     TPDLTIQYWGQPFFGSGDYSEFKRITDPVAASLDGRYEMLDGGVITYAIKDRKYSIDE
                     NLDKSPDYNFSNPDFTVSEFLHNLVVRWEFLPGSTAYLVWSQSRDYNTDDGRFSLSRQ
                     SYDLFNATKAHNIFLVKFSYRFGLR"
     gene            complement(5506..7839)
                     /locus_tag="PKK03_02480"
     CDS             complement(5506..7839)
                     /locus_tag="PKK03_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX83289.1"
                     /translation="MKPFRFIISAILLLAAAAGVSAQGTITGRVTGGPEGRPLPDAAV
                     ILDGTRGVTTGSDGRFIIDRVSAGTHTLEARILGYTTRNVTVDVRDGATGTVSFRLEE
                     EFIDAGAVIVTANRGASRIADVPARVTLISSAAVESRPVTTVDEMLSTVPGLNVSRSF
                     GIFSHKSSVTMRGLSGNEQARVLVMIDGVPVNKSDGGSVNWNLIDPAMVDRIEVVKGP
                     ASSMYGSNAMGGAINVITRRPSGELSGKITAGYGTYNTFTGRISLGQNMNPGSDRGFY
                     YLLNGFYRQSDGYITQSEFDQAANPYVVASDMLEYSASLKTGYNIRKGEFIEADFIAY
                     NDRRGTGELVWQQHGNTTDHDTYQFRTRYSVERERLSADVSLFWLQEDYKKVNEYLKD
                     DYTWYNVLSERLDAGLLSSVTWKAGSRHRLSAGTDLKAGSVDAADVYYTSTDIVYNRG
                     RMLSAGLYIQDAFSIIHDRLTLTAGLRYDLSMFRDGAFYIDTPSAETVFMRNIENREM
                     DNVTWGALSPRLALNYTPSEGTRIYLSAGRGFRPSVLDDLCRSGRIKGGFKLANADAS
                     PEYLANIEAGGDFTLSGKLRASLSAWYSRGRDFLYYVNTGDSIDMGYGLRPILVRTNI
                     PLVEITGAEAELNWPVSPAVFLGAAYGYSYSVISDYRPLDAGDPIDLTGNHLTDVPTS
                     TVSLTARWNNRFVNVGLVARYQGVMWVNDQNIFDEVVGSDRYPAYATVDIRLSRVFAE
                     KVSVDLGVQNLPDTKFYDSKGAVCPGRFITLELGYRF"
     gene            complement(7908..8807)
                     /locus_tag="PKK03_02485"
     CDS             complement(7908..8807)
                     /locus_tag="PKK03_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83290.1"
                     /translation="MAKRIQCFALLIIIALAGCVKDNPGTTPLILLKSGGAFTADGSA
                     VAPGGALRFGLSVSGGGGAITNLVVKRISDGVAVIEADKGMYISYGGLDTTLIYTRGY
                     GQTERWVFSVMNSYRDTASVSLTVLKGAGSAWGEISHHQSIRIGLQENTSLPHFVDLH
                     AGIAYDATGVTGHESEVDMAAFWYLTSGTSSPTLTCPAYSSALTYYPLFGSWSAKNQT
                     MYDYYTSDNNLVTPAQFDLATNDSLLVNAYRPGSVSGQSKFAYTGKVVPFRTADGKYG
                     LLRVIRADETPEGEMEIAVKIQK"
     gene            complement(8815..9363)
                     /locus_tag="PKK03_02490"
     CDS             complement(8815..9363)
                     /locus_tag="PKK03_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83291.1"
                     /translation="MKTRFALLMILPLVAVMISGCEKEKENPSVTLGAQANAAVPGFY
                     SVSLDKTYTMSQAADNAGMIDIFCFYEAATGNNIALASPGSGIRDIFTGDDMPDNWGT
                     KNTTYFFQTTLTATQFEAVQDGDALIETSFDTDNARKKAKDVQVGQVWAFRTQDGTYG
                     LLLATAVTQGTDGTVTFRVRTK"
     gene            9548..10324
                     /locus_tag="PKK03_02495"
     CDS             9548..10324
                     /locus_tag="PKK03_02495"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNX83292.1"
                     /translation="MKRKKEKVPGFDDIIFRDRNRDYGAYDLRRRYGSTMSVSLVAGL
                     LVGASFFLVPYFTSDPVEFQPGRRIDVIAMPDPDLPVPPVPPEAPPAPKAPAEAMNNV
                     RFVPPRVVIDELATGEGLMTADLLNRSITDGASTEVIPEDFTAVDPVAPVEPKPRVIV
                     QEMPSFPGGNEALLKYIMEHIVYPPDAVANGIQGTVYLRFVVSSTGEVTGVEVTRSVD
                     PLLDSEAKRVVSSLPRWKPGKQDGNPVPVWYSVPVIFVLR"
     gene            complement(10364..11077)
                     /locus_tag="PKK03_02500"
     CDS             complement(10364..11077)
                     /locus_tag="PKK03_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNX83293.1"
                     /translation="MTRVVAIDDEPLALQLVKGYVEKTPTLELAGTFDNPVDAVAFIQ
                     SAEVDLVLLDIQMPDLTGTELARVIAGGPKVIFTTAYEKYALEGFRLDAVDYLLKPFS
                     YAEFLKAVQKAEKLIAAERSQLPALEVNNDFLFIRSESRIRRINFSEIHYIEGLKDYV
                     KIWLKDERKPVLSLSTLKALESRLPGDRFMRVHRSYIVNLETVKVIERGRIVFGEVRI
                     PVTEQYIENFRKFLDRNFI"
     gene            complement(11090..12160)
                     /locus_tag="PKK03_02505"
     CDS             complement(11090..12160)
                     /locus_tag="PKK03_02505"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNX83294.1"
                     /translation="MAKKRKGLPVLLHATGWIILFILPQFLISGGLFNDGRTTRVVLF
                     NTLVFLILFYTNYFWLVPRLAQKKKWFAFLVLAASLIVVLGYASGKYYENLFAPPREV
                     MERIREANQRTQGDNRMPRRGGGMALYNFFITSFLVSGFAVGIRYAESAVRKEEEIKE
                     LEREKLNSELALLKNQVSPHFFFNTLNNIYSLIEINGKDAQEAVLSLSKMMRYMLYES
                     EQGNTRLSNEIEFMKGYIDLMKLRLSDRVKLTVSFPEDYADQDVPPLLFIPFIENAFK
                     HGVSAVGNSFIDIALGVEDGMITFIAANSIGGRASDGPQVASGIGLDNVRKRLALLYP
                     GRHDLRIDRQESVYTVKLTVRS"
     gene            complement(12207..13427)
                     /locus_tag="PKK03_02510"
     CDS             complement(12207..13427)
                     /locus_tag="PKK03_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX83295.1"
                     /translation="MNPRNLLKVAINALKRNKMRSFLTMLGIIIGVASVITMLAIGQG
                     SKKSIQDQIASMGSNMLFVMPGTMRMGGVQQGSSSSQRLTVADIKAIKMECDAVVAVS
                     PEVRSSGQVVYGNSNWPTTIYGGSEQYLEIRSWKVVSGRNITDTEAEGSAKVCLVGRT
                     VADELFGEGVDPTGETIRFKSIPFKIIGVLEEKGQNSFGQDQDDVIIAPYTTVQKRIL
                     AQNYIQSIQMSARSAEESDLAQSQVEEVLRRTHKLRENEDNDFEVRSQEELATTMTSV
                     TEILTILLGAIAGISLLVGGIGIMNIMYVSVTERTREIGLRMSVGGRSVDILLQFLIE
                     SILLSVFGGLIGIVLGFAASSIVEVLTSWPISVMWGSVILAFAVCTVIGVFFGWYPAR
                     RASELDPIDALRFE"
     gene            complement(13440..14180)
                     /locus_tag="PKK03_02515"
     CDS             complement(13440..14180)
                     /locus_tag="PKK03_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX83296.1"
                     /translation="MKTIIDITTLKKDYHVGEVTVHALRGVDLKINEGEFVAIMGASG
                     SGKSTMLNIIGCLDRPTDGTYFLDGVNVAGMSKNERAAMRNLKLGFVFQSYNLLSRTS
                     ALENVELPLMYNRVISARERREKAVAALEAVGLADRMGHMPNQLSGGQQQRVAIARSL
                     VNDPVVILADEATGNLDTRTSYEIMSLFQDLNAKGKTIVFVTHEPDIARCMTRSVVFR
                     DGHIIREEEVKNRLDAAELLRALPVEQD"
     gene            complement(14183..15613)
                     /locus_tag="PKK03_02520"
     CDS             complement(14183..15613)
                     /locus_tag="PKK03_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX83297.1"
                     /translation="MKRKNTFISIGLLAAVAVTLLSVHSCRKGSTYIYETAKVTKGSI
                     VNTVTATGTLEAITSVVVGTQVSGIVEKLYVDFNSPVKKGTILAELDKTALRSSVQQA
                     LATLDNSKAEMEYQASNYERSKALWEKNLIAKADFDQAKYNYDRSVATLKNSQAQYDK
                     AIVQLGYATIYSPIDGVVMNRAVDEGQTVAASFNTPEIFTIAQDLTQMQVEADIDETD
                     IGQVKTGQRVEFDVDAYAGEIFSGTVEQIRLSPVTTSTVVTYTVIINAPNPDQKLLPG
                     MTANIVIYAEEVKDVLIVPYKAVKFTPDAEFLAQQWKEMASKNGMAGGAGMSGGAGMS
                     GGAGMSGGAGIPGGTGRMPSGMSGSGAPGVAGAGFGMQGMPGGQMPEGFKLPEGFKMP
                     TMVWVKDDKGIHPVPVELGSNDGSNTEVKSGLEEGSEVVTKMTIASAKEKKVQEVSSN
                     PFMPTPPGRRNTQSSSRTGANAATRN"
     gene            complement(15641..16957)
                     /locus_tag="PKK03_02525"
     CDS             complement(15641..16957)
                     /locus_tag="PKK03_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX83298.1"
                     /translation="MRTKRFIAMAAALIISSVAVTAQTKQWSLTDCIGYALEKNIQVR
                     KAGVSVSLGELSLQQSKDNMLPSLSASLSENLGWRLETGASDETSLVGSSRTNASLSS
                     GLTLFNGFQLRNQVRQAGLDLKSLQYSADQTTESVSLNIMSAYLQVLYSEEQVINARN
                     QAEATREELALAAERLTLGAIANSDYLQVKTQLASEESSLATAVNNLKMAKLNLMQLM
                     EYPVDESFEIARPDIDAFISTAPAADAVSVYQEALEVKPQIKIASINRENASIDLEMA
                     RAGFLPSLSLSAGMSTGFTEAAEMGAQLKNGFSPSVGLSASVPIFRNNQNKTAVSRAK
                     YGITTAELDELNTRNQLRKEVEQAVLDAESALISYQAALNRYDSSVETYNLSEEKFKL
                     GAMNSVDFLIQKTNLTAAESNLLQAKYELVYSHKIIDFYRGVALTF"
     gene            complement(17090..18424)
                     /locus_tag="PKK03_02530"
     CDS             complement(17090..18424)
                     /locus_tag="PKK03_02530"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX83299.1"
                     /translation="MRRRNKQSGSIPLRVNTIFRSLRHRNYRLFFSGQSISLIGTWMQ
                     RIALPWVVYRMTDSEVLLGVVGFASSIPSFLLAPFAGVLIDRWSRYRVMLVTQIISMI
                     QAGVLAWLSLSGNLEIWHIVVLSVALGCINSFDMPARHSFVINMVNGKEDIGNAIALN
                     SMMFNGARLIGPSVAGIVLASVGEGACFLINALSYIFVIASLMMMRVTEVREKKERVP
                     MFREMKEGMDYVFGFAPIKHIILLLGLVSLMGASYQVLMPVYAKDILQGDSHTYGFLM
                     GGAGAGALLGAIYLASRDTVLRLGRLIPAATALLSAGLIAISLSSSFFISMFLIFFTG
                     LGMMAHTAASNTILQTIADDDKRGRVMSFYTMALMGTAPFGSLLAGWMAKVLGTPWTI
                     FIGGAVCLLGALVFYRRLPELKRLVRPVYVRMGILPEVAEGIRTASEPGSGT"
     gene            complement(18463..19260)
                     /locus_tag="PKK03_02535"
     CDS             complement(18463..19260)
                     /locus_tag="PKK03_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83300.1"
                     /translation="MMTSLCNRLCSSAAVICGLFLCNSISISGQKIIRDTLTGKKIKE
                     IHRDAFMYNANDTGAVAQKDIPEIQLTALKAKAGAERWWYAWLAGYSAATVGQGVVCF
                     TSEDRILRQDMALGAATTFLGAMGQVITPVLTKNPAYPSYREYASGEASLSADQAAAL
                     LKALSEREKEGRSWKTHAIAGVVNLGSGLITWLGYKRTFMDGLENFAINTLITEAQIW
                     TQPMKAVRDYEKYCQTAGPGTGTMTSRRDGEWSWSIYPGGFHVRYVF"
     gene            complement(19257..20492)
                     /locus_tag="PKK03_02540"
     CDS             complement(19257..20492)
                     /locus_tag="PKK03_02540"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX83301.1"
                     /translation="MKETEFRVYGYRWVMLSVYMLAIAVNQIMWITFAPITKDAAAFF
                     GVTDLQIGLLSMSFMIVYLIVSIPASWIIDTYGIRAGVGAGVVLTGVFGLLRGIFASD
                     YNLLLIFQIGIAVGQPFLLNSITKVASRWFPVSERATASGLGTLAMYFGILLGMSLTP
                     FLVNGRNIDGMLKLYGMVSMATAIIFFILARERPVTPPCRPDQEERALAIDGMRLLFR
                     NRDFLWLMLIFFIGLGVFNSVTTWIENILSPRGFSAEQAGITGGLMILGGVVGALIMP
                     ILSDRFRKRVPFIQLALAGATISLAGVTFTGNYTLLLVSAAAFGFFLLSSGPIGFQYG
                     AEVTFPVSEGTSNGFLLLMGQVSGIAFIFGMDWFKSPVTGSMTMPLSVLCILMLISYF
                     LSSRLKESAFLAGNQKGDV"
     gene            20742..21014
                     /locus_tag="PKK03_02545"
     CDS             20742..21014
                     /locus_tag="PKK03_02545"
                     /inference="COORDINATES: protein motif:HMM:NF009508.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI-like protein"
                     /protein_id="HNX83302.1"
                     /translation="MFYILFYKTVENYIERRAPFREDHLGLVHEFHNDGRMFMAGALA
                     EPADGAVLIFHGDTPEAAHEFVSRDPYVKNGLITEWSVRPWMVVTG"
     gene            21040..24411
                     /locus_tag="PKK03_02550"
     CDS             21040..24411
                     /locus_tag="PKK03_02550"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNX83303.1"
                     /translation="MKNNQSHSGMAINLTGLLMMILLAPALTAGAQDPFMQYPDIQGN
                     IIVFASGGDLWKVPAEGGTACRLTFSDGRESVPEISPDGSLIAFTGEYDGNADVYIMN
                     IDGGNITRLTWHPGTDEVVGWNAVKNKIMFTSGRNSPSRYVKMYLVSPDGTGLEEMIM
                     YDAARGSFSPDGTKIAYNKDSQDNATWKRYRGGRAQEVYIYDLETDKETNISNYDGSD
                     RWPMWIGDKVYFSSDRDRVLNIWSYDPGTGKIEQVTKHTEYDVRHPDFGGSQIVYELG
                     GDIWKLDVTSGVTARVPVKILTDMEERRPFMKDVSRNITRADISPSGNRALIVARGDI
                     FTLPEKEGPVRNLTASPGARDKDAVWSPDGKRIAYFSDASGEYELYVIEPDGKEKPLK
                     LTSLGAGYRHTIKWSPDSKKIAWTDQTLTLWFIDVATKAITKVDKEEYENVDVSLDIK
                     SIFDYSWSPDSRFIVYSKMNEAFMYQLYVYGLEAKSINCISNGLFHDFNPVFTNDGEY
                     IIFISNRRFEPTYCDLEWEMVYQKIAGLYAITLKKDGKSLMPFKSDEEPGATTVVTAP
                     PAIPVAGKGSAAAKGNAAGKTPTAQPAMRAPAAQGQGSTVTVQIDFEGITDRTEALPL
                     EKGNYRSLAVNDRSLFWLNSDEGNFNRFEVAGHGPMNLFSWSFKSAKQSALTDGIDDY
                     RLSADGSTILLRKDGGVSLMPADGGNASPLKLTDLKVWYDPVSEWKQIFNEAWRMERD
                     YYYEPGMHGQDWPAMKQKYEKLADRATCRQDLTFIIGEMIAELNTSHTYVYGGDSKRR
                     ADQVNVGMLGADYSVDSQNNLYRFAKIYREKDWSREAWPPLAKPGINITEGDYLLKVN
                     NTEIKADREVCSYFVGLAGKQVTLTVNSRPTLAGAREVTVVPAGSESSFRYMDWLESN
                     RKAVDKASGGKIGYIYLPDTWNGSATDFPRYFYSQTKKEGLVIDGRFNGGGLDPEIFL
                     QRLQKKPHAFWTRRQSVDQPIPHLAVDAQMALITNRYAGSGGDELPYEFRWFGMGPII
                     GTRTWGGLVGVSQFIELIDGGTITAPDYRIYNDKGEWVVENEGVTPDIIIDVDSKKYS
                     EGYDTQLMKAVEVVMKKISEEPRQWPQHKPYPVDR"
     gene            complement(24500..26968)
                     /locus_tag="PKK03_02555"
     CDS             complement(24500..26968)
                     /locus_tag="PKK03_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX83304.1"
                     /translation="MLFNDHKYGMSAREMKETGSTDSMKSWKRIGFIATVVLVVSFPV
                     YLVRTALEKQGEEREQGPSFTGAATCIECHKREYDLWKGSHHDLAMDIATDSSVLGDF
                     NNYEFRHRGEVHRFFRKGEKYFVNTEGATGRFEDFEVAYTFGYTPLQQYLVPFEGGRL
                     QCLPIAWDTERGRWFHLGDTVYTDEEVGPGNWLHWTRQAQNWNGMCADCHSTNLKKNY
                     NPATKTYNTTWSEIDVSCEACHGPASEHLVWAALPEGSRPADVNTGLIVRTRDLNNEE
                     LLNVCARCHSRRAIMGNYADDNNDLLNYMIPMLTAQPLYHVDGQILDEVYEYGSFTQS
                     RMFEQDVMCSDCHDSHSARTKEPDNRLCLQCHRPDIYDTPRHHFHKMDPAVAGGQLMS
                     KANVYSGNVAGGQPAGKGTQLYRESTGTGRLINRGEPKYVEGTGAQCVNCHMTGRYYM
                     GNDYRRDHSFRIPRPDLTAKTGVPNACNDCHKDKSTAWSQSYILKWYGERTRPHYGET
                     LAAAEKGDRSVISDLVLYSENELFPLMVRATAVRFLGRFNTPESNRAVERALTDPASL
                     VRHTAVMSYNPENIASYEKLMMPLLNDPVRGIRAEAAIRLSEVREDQLSPATRKARRA
                     ALDEYREINLYNADFPGGLFNLGIMYANAGDFPKAEAAYREALGVDDQFYMAKVNLAT
                     VCAQQGRTAEAEKLLRELLRDHPGLHELNSSLAMLLAEQGRYEESREFFLKAARLMPE
                     QTRILYNLGLLENTLGNTARAEEYLLKALNREPGNYDYLYAMATFCLEHGMKDKALKY
                     ARQLADRFPGDQAARYLIDAVGKR"
     gene            complement(26958..27473)
                     /locus_tag="PKK03_02560"
     CDS             complement(26958..27473)
                     /locus_tag="PKK03_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNX83305.1"
                     /translation="MSGRIKDTGSEHRKGRRNFTRRLWKILGIVALAEFLWFILSLLK
                     PSKETHKSRPADTIRVIGSVEEFPAGSVTPDRVNRLYIVREKDGAFLALSLTCPHLGC
                     SVLWNAETQQFDCPCHSSAFDHRGIVLSSPAPRPLDYLPVIIEEGKVKVDLGSMTKRK
                     RFESNQLTYAL"
     gene            complement(27497..28798)
                     /locus_tag="PKK03_02565"
     CDS             complement(27497..28798)
                     /locus_tag="PKK03_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNX83306.1"
                     /translation="MTEHHATEKREKSRFGLILHLHPRKVKASTLRFTLTFGLGGMAA
                     LLVVIQVVTGLLLKFHYEPSPENAYNSILNLQESLLFGKMLRNIHHWSAIAFVWVAFL
                     HMLRTAFTGAYRPPRDGTWITGLALMLLVVLSNFTGYLLPWDQLSYWAVTVGTSLLSY
                     IPLAGEPIRRALLGGAEVGQPTLTNFFNLHTGVIPLMIIALMAWHFWRIRRAGGVIVA
                     DRDKESPMADTKPYLVNREFVVAITLLAAIFIISSLLDAPLRERANPAFSPNPAKAPW
                     YFMGLQELLIHFHPLFAVFILPVTALVAAVWMPYIKSTDRNQGVWFLSPAGIRSARMA
                     AATGMIMAIIFITMSEILPDPEVLLPEVTPLLTTGLVPFILAAGTVWLLLKYLRRRYS
                     LKRSEMIQSIVVLMVVSYAVLTVTGIFFRGGGMNLVWPWNV"
     assembly_gap    29049..29148
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            29149..29223
                     /locus_tag="PKK03_02570"
     tRNA            29149..29223
                     /locus_tag="PKK03_02570"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29184..29186,aa:Val,seq:cac)
     gene            complement(29298..29849)
                     /locus_tag="PKK03_02575"
     CDS             complement(29298..29849)
                     /locus_tag="PKK03_02575"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNX83307.1"
                     /translation="MKENDKGDVSRPTVNEYLDALERLMIIENQPAWSTHIRSSASLR
                     KSPKMHFTDVSLSVAALGADKESLLRDVKFSGFLFESLVTHDMRVYARANDACVCHYS
                     DSSGLEIDCIIQKRNGDWAAFEIKLGMGMINEAASNLKKLIKVLDIKSIGPPKSLNII
                     VGTGVSHTRPDGINVISLASLGV"
     gene            30008..32473
                     /locus_tag="PKK03_02580"
     CDS             30008..32473
                     /locus_tag="PKK03_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNX83308.1"
                     /translation="MMNKNPLSSAITGNMNSPRLLCSRITLTLLFLLPLLSASAEYRG
                     VVYNDLNRNRQHDAGEKGMSGVAVSDGLNVVLTDRQGAFRLPGTPETRFIRITVPAGY
                     ISSGSFYRKADGQESGYDFGLVPFNASGNKTVRFLQLADTETGYDFGWIAPIRDYAAN
                     EEVSFIIHTGDICYEYGLNFHGSNVMAETMGVPVYYCVGNHDLVKGNYGEELFEKNFG
                     PAFYSFDAGNTHFIITPMLSGDYKPSYTKEDVYRWMKNDLRLVDPSKNLVVFNHNLLT
                     MGDKFIYGINEAEHINLGEHNLKAWIYGHWHSNYFIRHGNSGIVSFCSAPPNKGGIDH
                     SVSNFPVYEIDGEGNIAARPRYNYLNNHFAAVSPSGNQAVIDDSGKLLVSVNTYSTVS
                     PTDAAEFMIEGLGEWKALSRESDWNWSGKSDAEDMKRGIPYHINFRVRLKNGDTFSSV
                     QNFIFSEDASIDQSAEVTLKDPLKLKWITNAGTGSGICPPVYSDGTLYVAVYEDYGTN
                     NKIMALDGSTGDLKWQFLTENPVKNTLSLSDGHLFATDEGAIAYSINAGNGELAWKRN
                     LGLSTFPLPVNGGVVHNGVYYCGYGNYLSALDSHDGHLIWKNDSWNGGEGTTSTMAVH
                     GNTLIAGANWRALYGHDLQSGAKKWEVSSDGIRFCNGTAAFIDDTMFFAAERALIRMD
                     PGTGSIYKVNPVPYDMQVASTPLVTDKIIVTGTSAEGLVAWDRYSSKELWKVKPVTSL
                     LYTAPYSKPPASTVETSPIAVGDMIVFGASDGYLYAIDSGNGRIRCKINLGAPVFSTA
                     CLAGDLLYVADFSGNISCFYLKK"
     gene            complement(32617..32979)
                     /locus_tag="PKK03_02585"
     CDS             complement(32617..32979)
                     /locus_tag="PKK03_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83309.1"
                     /translation="MQRKSVYALITAFALLVLLSVIPGCDIETENMITVTMINGSSED
                     VHMWTNGESIDPSNKLAPGKLRNYVWDVKDTENFPEITFTIYAGQNGQTLTSKQFVLP
                     NATGSLTVKYVGGSLVKQ"
     gene            complement(33103..34506)
                     /locus_tag="PKK03_02590"
     CDS             complement(33103..34506)
                     /locus_tag="PKK03_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNX83310.1"
                     /translation="MTDQRSLSRTLGLGYVVIFVIANIIGSGVYKKIAPMADELQSSV
                     WILLVWIAGGLITLFGALSNAELAGMLADTGGDYVYLKKIYKRFFSFLYGWSLFAVIQ
                     TATISSLAYVLVQSLNSVIPIPDLLPALHDFSIGGVFFPFQDFGIKLAAIIVIMLLTL
                     LNMSGLRSGAWTGKAIFFFVCAGLLVIVIAGFTTNNPRPENFMNFRELGEGTVTLSSF
                     YTAMLAAFWAYQGWVSVGFIGGEIRNANNNIPKGIAIGVLVVIAVYVLVNLAYMRIMS
                     IPELVQVHEAGNQIAAIEAVRSLWGQGGVLFISVLILITTMGCLNASILTGSRPYYAM
                     SQERLFFSGIGKLNRFNVPGNSLLWQGIWASVLVLSGTFDQLTDMLIFAVYIFYGAMA
                     TGVFILRRRMPYAHRPYRVWGYPVVTAIFVLFCIGLFFNTIITRPREAMIGLSLILAG
                     IPVYFIFNRKTRDERDG"
     gene            34732..35319
                     /locus_tag="PKK03_02595"
     CDS             34732..35319
                     /locus_tag="PKK03_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83311.1"
                     /translation="MKKPVITLLAMLLCTGATAQWKAQTADTYEKTYRMAFVVSASGT
                     ETLRIMRDVTPGTSTKGANPYDQISGQILLNKNVGTEYSVNTIVLRFDESTKLYIHQP
                     ADLKQGWDANAGRYIIESDWQVWRTGDTRNKGAGRAAAGSGTNLKKEILDMMKSGKTV
                     HCQTILIHKMHDTQTIINSEFKLQNSTRSINYLFQ"
     gene            35394..37286
                     /locus_tag="PKK03_02600"
     CDS             35394..37286
                     /locus_tag="PKK03_02600"
                     /inference="COORDINATES: protein motif:HMM:NF003780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HNX83312.1"
                     /translation="MKKITLVLFVMAMIIGYHPDLFAQKKNRKAESAAQPAADMKPEA
                     QKQVQAATVVPVIVNGEAQKIPAFENAGDWIREDLWVETEFDTDGDGKNDRMHVDVTR
                     PKQTDTEGIKLPVVYESSPYFAGTAGNNKEYFWNVRQELNTVPKPHIYPPQIERRGER
                     PVISNSQVKAWLPYGFAVVHSSAPGTGLSQGCPTIGTRIEALAPKAVIDWLNGRAKGY
                     TTPDGSVEVKAYWATGKVGMIGTSYNGTIPFAAATTGVEGLEAIIPVAPNTSYYHYYR
                     SNGLVRSPGGYLGEDADVLYDFVFSNPDNCDYCDSVVRDGEMAAGLDRITGDYNEFWA
                     KRDLMNYMKPYKAATLMAHAFNDWNVVPSHSYRFIEKLKEMGVPLQIYYHQGSHGGEP
                     PFKMMNRWFTRYLFGIENGVENDPKAWIVREGAERLSPTAYTDYPNPDARPVTLYLTG
                     GAPEAGGLVMTKPAKQEKETLIDNYSFTGSSLAQAEYSNHRLLYLTPVLTEPVHISGV
                     VKIKIRAASSKPAVNLSVWMVALPWDDGRIMRMTESIITRGWADLQNYRSLTESEPLV
                     PGKFYEMSFGLEPDDQIIPAGKQIGLMIFSSDKEFTLHPIPGTELTVDLAGTEVTIPV
                     VGGKIN"
     gene            37469..>38241
                     /locus_tag="PKK03_02605"
     CDS             37469..>38241
                     /locus_tag="PKK03_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNX83313.1"
                     /translation="MKNQVFIILCLLTFAVLSSCSKDNGEEPDLTPENLTLTAKSSEV
                     IAYSNEFGVDLFTKVALAENKNFMLSPLSASAALTMLLNGCAEDTYDQLKATLKYPEQ
                     MTISEINEVYKNLVSQLLVIDPKVKLALANAIFYRQGFTVKPPFLSTMDNDFNAEIAG
                     LDFSLPSALTTINKWASDNTNGKIPEVLNEISPEAVMFIMNALYFKGDWSYQFDKDLT
                     SDRAFYTDGSNSVNVSTMKGEVGSKVTGGAGYSAIELPYG"
BASE COUNT         9349 a         9916 c         9708 g         9168 t
ORIGIN      
        1 gtctttatga cggacttgca tcttgatttt taacccttac tgccatgaaa agaattgcat
       61 ttattgcggc tgtgctactt ctgtgtactg ttgccatgac cgctgcggcg aaccaggact
      121 ccatcccggg aaagacctac cgggcccgat tcacctcctc accacccgta attgacggat
      181 caatcgatga tgccgcgtgg caggccggtg aatggcaggg cgactttacc cagtatgagc
      241 ccgacaacgg agctgctcct ttgcagaaga ccgaattcat cctgctgtat gatgacaaca
      301 acatttacgt tgccttcaaa gcctgggaca caagtgctga cagtatcgtc aggcgcattt
      361 ccagaagaga taatggtgaa ggcgattttg tagggattgg tttcgacagc taccatgacc
      421 tgcagaccgg atttgccttt gtcaccaacg cggcaggagt aaagaatgat ttcatctggt
      481 cacaggacgg ccggatggaa gatgacacct gggatcccat ctggttcacg ggagcaaaag
      541 tctatgaatg gggatgggct gcagagatgc ggatacccct cacccagctg aggttcagga
      601 tctctgacga tggcgtctgg gggctggagg tcttccgtcg catcttccgc aaccaggaga
      661 tgtctgtatg gcagcatatc gaccggaatg cttccggcct ggttcataac ttcggcacac
      721 tctcagggct gacagggatt aagcccagaa aacaggctga catcaccccg tttgtcgtgg
      781 ggagcctgga gacattcgag aaagatgatg gcaacccgtt cgccaccggt accaactgga
      841 aatacaacgg cgggcttgat gccaagttcg gcctgactaa caacatgacc cttgacctta
      901 ccctcaatcc cgatttcggg caggttgagg ctgacccttc agaggtgaat ctcacagcct
      961 atgagacatt ctttgaagag aagaggccgt ttttcattga gggaaaaaat attaccagct
     1021 tcagaacagg tctcggtgac ggagaccttg gctttgacaa tctattttat tcaaggagga
     1081 taggacgaag cccgcacctg ggtgttgaca ctgacgatga tgaattcgcc agggtaccgc
     1141 gcgtcacgcc aatcataagc gccgccaagc tgacggggaa aacgcctgac gggctgtcaa
     1201 ttggaattgt ggaggcagtg acagcagagg gcagggcagt gattgattca ctgggtcaca
     1261 ggagtgagca gacggttgag cctttcacca actaccttgt atcaagggtt atgaaagact
     1321 ttggtggcgg acgcacaatt atcggcggtg cattcacaaa cacccaccgt ttccttgaca
     1381 ataccgggat tgacggtctg gtccgtaatg ccaatacggc cggaattgac ttcacccggt
     1441 atttcggtga agagcgtgac tggttcgtgt cagccactgc agccggaagc aatcttacag
     1501 ggtctaaaac agcaatagag gatcttcagc gctcatcaat acattatttt cagagaccgg
     1561 atgcagatta tgtagagtta gacccggaaa ggacttcact caacggcttc ggaggcaatg
     1621 ttcaggccgg taaaatgggc gggcactgga atttcatggc tttcacatac ctgaaatcac
     1681 ccgggctgga ccttaatgat gtgggatacc ttcagagcgc cgatggaata ataagcggcc
     1741 tgtggtctgc ctacaggttt gacaagccgt tcagtatatt cagacagata aggcccaacg
     1801 ccaacttctg gaccggatgg gacttcgggg ggacccatct gttcactggc gggaacctga
     1861 gcctctatgt tcagttcaaa aacctgtggt ggacaaactt cggagttaat tacgaaggta
     1921 attccctatc caacaacctc ctccgagggg gaccggcaat gctttacccc tcctctgtaa
     1981 acagctggtt caatcttggt acaagcgact caaaaaaggt atccgcatct gtgtttgcca
     2041 acgcatcccg cggagcagaa aatgtctcag aaagatattc ggccggaata gactttaccg
     2101 tgaagcccgg gcagtcattc acagcctccg ttgaaccttc ctattccata aaccgtaacg
     2161 agatacagta tgtaacctgc accgatgacg atgaacgcta tatcctgagc cgtatagatc
     2221 agcagattgt cagtatgtct gtgaggctga actataatat cactcctgac ctgactgtgc
     2281 agtactgggg acagccgttc cttgcagcga tggattacag caggtttaag gcagttacag
     2341 atccgagggc agagacactc gccggtcgtt atcaccttat cagtgaagat gaaatgacct
     2401 atgattctga cgatgacatg tacctggttg acgaagacta tgacggcatt actgactaca
     2461 gttttgacaa tccggacaac aattatgatc agtttctttc gaacctggtt ctcagatggg
     2521 aattcagtcc cgggtcaacc ctgttccttg tctggtcaca gacccggaac tacagtgatg
     2581 aaaccggttc cttttcgctc agtcacaatc ttgacaatct ctacacctct aaaaaaccct
     2641 atgatgtatt cctggtaaaa ttcacatacc gtttcggttt gcggtaatcg ccgctgatcg
     2701 caggtccgga agagcgacgg gtatcactcc gtcgctcttc atgttttaca attcagcccg
     2761 ggaaagattg cagaaatttt ccctaacttt gtattagctt tactgtccct gaatcctcat
     2821 caaaatgaaa agtggccttc tcattctttt ttcatgtatt accctcgttc tgccagccca
     2881 ggagtacgag aaaaaagaat ataccgccag ccgcactgaa aatccgccgg tgatcaacgg
     2941 tgtgcctgac gatgaagaat ggacagaagg tgaatgggcc ggtgacttct ggcagtatga
     3001 cccggccgaa ggggcgcccg tcagtcagaa gacagagttc aaactgcttt atgatgatta
     3061 caatatctat gttgccataa aagcctatga tactgcgcct gatagcatcg tttcacgcat
     3121 gacaaggcgt gatgatactg agggtgacga ggtgtctgta gccctggatt catactttga
     3181 ccagcgcact gcattcggat ttacggtaag cgcggcaggc gtcaagggag acctgatgtg
     3241 gattgatgac gggctgacag aggatgactc attcgatccg atatggtacg tcaaaaccgg
     3301 aatatttgac tggggatggg ccgctgaaat gaggataccg ctcacacagc tccggttctc
     3361 tgccagcgac cagcaggtat gggggctgga ggtgctgagg gaaatatatc gccataatga
     3421 gacggttatg tggcaaccca ttgccaggaa cgcttcaggg ctggttcata atgccgggct
     3481 gcttaaaggc ctcacaaaca tcaggccccg gaagctgttt gacctgaccc cctacggtgt
     3541 ggcaagactg gaaacctatg aaggggaaca gggcaatccg taccatgacg gaactgatat
     3601 caaaggaaat attggccttg atgcaaaaat aggggtgaca aacaacatga tcctcagtct
     3661 gtcacttaat cccgatttcg ggcaggttga ggctgacccg tcagaagtta acctcacagc
     3721 ctttgaaact ttcttcagtg aaaagcggcc tttcttcatt gaaggtaaca acataacgga
     3781 ctacaacctt ggattgggca gcggcggccg cgggaatgac aacatgttct attcgagaag
     3841 aataggcagg cagccacggc tgagccattc agccggccaa ggtgaatatg cctatacccc
     3901 cgcgttcact cccataatcg gatcggcaaa gctgaccggc aaatccgcag gcggactctc
     3961 ggtgggtttc attgaagcgg tgactgcaaa tgtgagtacc aggatccatg atcccctgac
     4021 aggtgagaca acattcagga ctgccgagcc gcttaccaac tacggcgtgg gcagggtgca
     4081 gaaggacttc agcgggggaa aaacaataat cggcgggatg gtgacaagca cgatcagatc
     4141 acttgatgcg gaaaccgagg attacttcca taagtcagca acctctgccg gcatcgactt
     4201 caccagatac ttcggtaaca tgaattacat cttccggttg cgcactgccg tcagtaatgt
     4261 cacaggaacc gatgaaatga ttgccggcac ccagagatct gttatccaca acttcgccag
     4321 gcccgatgca gattatctcg attatgacat cacccgaacg tcacttagcg gattcggagg
     4381 aaaccttatg gcgggcaaga tcgggggcaa ctggcagttt atttacctct cgagctggaa
     4441 atcaccgggc tttgagatca acgatatagg ttatatgctg atggctgaca tgcatatggg
     4501 agtgggagta atcaactata acatttataa gccattcagt gtattcagaa gcatgagctt
     4561 cgggaccaac ctgatacacc tgatggactt cggcggacat accaatgccg tagccgtctc
     4621 ccagtcgtgg acagcaaact accagaacca gtggcggacg ttcctcagcg gccagctgac
     4681 cagtcccgaa actgataacc accttttgcg agggggtccg gcaatgaaaa tgcccggtca
     4741 actgtatctg tcggccagca ttgattctga ctggcggaaa aaactgtcag gtgaggtgga
     4801 tttcagtgtt acccggactt tcaatgatgt gatgaccagc tacgagctga gctttgagct
     4861 ggagtacaga ccgtttaata ccctgactct caatattgag ccagggtgga gccggactgt
     4921 taacatgatg cagtatgtca ccttcaggca tcttgagggt ctgaatgatc aatctgagag
     4981 gtatattttc ggttccatag accagaagat atatagtatt tcgataaggg cagatttcaa
     5041 catcactccc gacctgacaa tccagtactg gggtcagcct ttcttcggat caggtgacta
     5101 cagcgagttc aaacggatca ctgaccctgt tgctgccagc cttgatggcc gctatgagat
     5161 gcttgacggc ggagtgataa cctatgcaat caaggacagg aaatacagta tcgatgagaa
     5221 tctcgacaag tcaccagact ataatttcag caatcccgac ttcactgtaa gtgaattcct
     5281 tcacaacctg gtagtgcgct gggagtttct tcccggatca acagcctatc tcgtctggtc
     5341 acagagcagg gattacaata cggatgatgg ccggttcagt ctcagccggc aatcatatga
     5401 tctcttcaat gccacgaaag cacacaacat ctttctcgta aagttctcgt accgcttcgg
     5461 cttgcgctga ggtgtacggg aaacaccggg ggccattccg tgatatcaga acctgtatcc
     5521 cagctccagg gtgatgaacc tgccggggca gacggcaccc ttactgtcat agaatttggt
     5581 gtcagggagg ttctgtacgc cgaggtcaac tgacaccttt tcagcaaaca cccttgacag
     5641 cctgatgtca accgtggcat atgccgggta acggtcactt ccgacaacct catcgaagat
     5701 attctggtca ttgacccaca tgactccctg gtaacgggca acaaggccga cattgacaaa
     5761 ccggttgttc cagcgagctg tcagtgacac cgtcgaagtg gggacgtctg tcagatggtt
     5821 gccggtcagg tcaatggggt cgccggcgtc aagcggacgg taatcagaaa ttaccgaata
     5881 actgtaaccg tatgctgcgc caagaaatac cgccggtgag accggccagt tcagttcggc
     5941 ttcagcgccg gtgatttcaa caagcgggat gttcgtccgt acaaggatgg gcctgagccc
     6001 gtatcccatg tcgattgagt caccagtgtt tacataataa aggaagtccc tgccacgcga
     6061 ataccacgct gacaaggatg ctctcagttt gcctgaaagg gtgaagtcgc cccctgcctc
     6121 tatgttggcc aggtattcag gcgaagcgtc agcgttcgcc agcttgaatc cgcctttgat
     6181 ccggcctgac ctgcagaggt catcaagcac tgacggacgg aatcctcttc ctgcagagag
     6241 ataaatcctt gtgccctctg aaggggtata gttgagagcc agcctggggc tgagggcacc
     6301 ccaggttaca ttgtccatct cacggttctc aatattcctc atgaagaccg tttcggccga
     6361 gggagtatcg atgtagaatg caccgtcacg gaacattgag aggtcatacc tgagtcctgc
     6421 agtaagggtc agcctgtcat ggatgattga aaaggcatcc tggatataaa gaccggcgct
     6481 aagcatcctg ccccggttgt aaactatatc ggtcgatgtg taatagacgt cagctgcatc
     6541 aacacttcca gccttcaggt cagtgcctgc cgacaaccgg tgccggctgc cggccttcca
     6601 ggtgactgaa gagagcagac ccgcgtcgag tctttccgac aacacattgt accatgtgta
     6661 atcatcctta aggtattcat ttactttttt gtagtcttcc tgcagccaga agaggctcac
     6721 atcagccgaa aggcgttcgc gttcaacgct gtacctggtc ctgaactgat atgtgtcatg
     6781 atcagtggtg ttgccgtgct gctgccacac cagctcaccc gtgccgcgcc tgtcattgta
     6841 tgcaatgaaa tcagcctcga taaattctcc tttccttatg ttgtagcctg ttttgaggct
     6901 ggccgagtac tccagcatgt ctgatgcaac cacatacgga ttggcagcct ggtcgaactc
     6961 cgactgtgtt atgtagccat cgctctggcg gtagaatccg ttgagaaggt aatagaatcc
     7021 cctgtcactg ccgggattca tgttctggcc aaggctgatc cttcctgtga aggtattgta
     7081 tgtgccatag cctgcggtga tcttaccaga tagctcacct gagggcctgc gggtgataac
     7141 gttgatggca cctcccatgg cgttgctgcc gtacattgat gatgcaggcc ccttgaccac
     7201 ctctatcctg tcaaccattg ccgggtcaat aaggttccag ttgactgaac ctccgtctga
     7261 tttattgaca gggacaccgt caatcatgac cagcaccctg gcctgttcgt taccgctgag
     7321 accacgcatg gtgacacttg atttgtgtga aaagatcccg aatgagcggc taacgttaag
     7381 tcctggcaca gttgacagca tctcgtctac ggtggtaacg ggcctcgact cgacagctgc
     7441 ggaactgata agggtgacac gtgccgggac atcagcaatg cggctggcgc cgcgattggc
     7501 ggtgactatc actgcaccgg cgtcaatgaa ctcctcttca agccggaaac tgacagtgcc
     7561 tgttgcaccg tccctgacat ccactgtgac attccgggtg gtgtatccca ggatccttgc
     7621 ctccagggtg tgggttcctg cggataccct gtcaattatg aacctgccat cactgccggt
     7681 ggtcacacct cttgttccgt caagtatcac cgcggcatcc gggagcgggc ggccttccgg
     7741 accgccggtg actctcccgg taatggtacc ctgggcagag acaccggcgg cagcggccag
     7801 aaggagtatt gcagaaataa taaatctgaa gggcttcact attaatggtt ttaatgtatt
     7861 ctgtttccgg atctttacat cgatcttcat ttcgcctgga cttgaatcta tttctggatc
     7921 ttgacagcga tctccatctc cccctccgga gtctcgtctg cacggatgac cctgagaagc
     7981 ccgtatttac cgtcagcggt ccggaaaggc accaccttac cggtgtaggc aaacttcgat
     8041 tgcccgctga cactgccggg ccggtaggcg ttgaccagca ggctgtcatt tgttgcaaga
     8101 tcaaactggg caggagttac aagattgttg tcagacgtgt agtagtcata catagtctgg
     8161 ttcttcgcgc tccatgatcc gaacagggga tagtatgtaa gtgcggagga atatgccgga
     8221 caggtgagag tgggtgatga ggtgccggag gtaagatacc agaaggcggc catgtcaacc
     8281 tctgactcat gtccggtaac accggtagcg tcatatgcga tgccggcgtg aagatcgacg
     8341 aagtgcggca gggaagtgtt ctcctgcaac cctatcctta ttgactggtg atggcttatc
     8401 tcaccccagg cagagccggc gcctttcagc accgtgagtg atactgatgc cgtgtcacgg
     8461 tacgagttca tgactgagaa cacccaccgt tcagtctgac catagcctct ggtatagatc
     8521 agtgtggtgt caagcccccc gtaactgata tacatgcctt tgtcggcttc aatgacagcc
     8581 acaccgtcag agatcctttt aacaaccagg ttggtgatgg ccccgcctcc tccggatacc
     8641 gaaaggccga acctgagtgc tcccccgggt gccactgccg agccgtcagc ggtaaaggct
     8701 ccgcctgact ttagcagtat caggggcgtg gtcccggggt tatccttgac acaaccggct
     8761 aaagcaatga tgatcagtaa cgcgaagcac tgaatccgtt ttgccatggc agaactactt
     8821 tgtccggacc ctgaaggtaa cagtaccgtc tgtgccctgt gtcacggcag tagccagcag
     8881 gagtccgtag gtgccgtcct gggtcctgaa cgcccagacc tgtcccacct ggacatcctt
     8941 tgcctttttg cgggcgttgt cagtatcaaa ggaggtttca ataagtgcat caccgtcctg
     9001 tacagcttca aactgggttg cagtcagtgt ggtttggaag aaataagttg tattctttgt
     9061 cccccagtta tcgggcatgt catcaccggt aaaaatatcc cttatgcctg aacccggcga
     9121 tgcaagagcg atgttgttgc cggtcgcggc ctcatagaag cagaatatgt caatcatgcc
     9181 ggcgttgtca gcagcctggc tcatggtgta tgttttatca agtgaaaccg aatagaatcc
     9241 gggtactgcg gcattcgcct gtgcacccag ggtgactgac ggattttctt tttccttctc
     9301 gcagcctgaa atcataacag caaccagtgg cagaatcatt aaaagggcaa accttgtttt
     9361 catttctttc tttctttttt cgttatgcaa ttatttacac atcgctggca aagatagcat
     9421 gcggttctca caaataaatg atacaaatca tattattgtg atttgttaaa cgaccacggt
     9481 acaatatttg atgtttatta tgcaagaggc gcctcttcat taatatttta aacttaaatc
     9541 ttgaattatg aagaggaaaa aagaaaaggt tcccgggttt gatgatatta tcttccggga
     9601 tcgcaacagg gattatggcg cttatgacct gcggcgccgt tacggttcaa ctatgagtgt
     9661 ttcccttgtt gccggactgc tggttggcgc ctcctttttc ctggtgccgt attttacatc
     9721 tgatccggtt gagtttcagc caggcaggag aattgatgtc atagcgatgc cggatccgga
     9781 tcttccggtt ccgcctgttc cgcccgaggc tcctcctgca cctaaggcac ctgctgaagc
     9841 gatgaacaat gtgcggttcg ttccccccag ggtggtaatt gatgagctgg caacaggcga
     9901 ggggttaatg acggctgacc tcctgaaccg gagcatcacc gacggagcat ccacggaggt
     9961 gatccctgaa gactttaccg cggtggatcc tgtcgcacct gttgagccaa aaccccgggt
    10021 gattgtccag gagatgcctt cgttcccggg cgggaatgaa gcattattaa agtacatcat
    10081 ggaacatatc gtctatccgc ctgatgctgt tgcaaacggc atacagggga cggtgtacct
    10141 gaggtttgtg gtcagcagca caggggaggt gacaggggtg gaggtcaccc gcagcgtgga
    10201 tcccttgctt gacagcgaag caaaaagggt tgtaagctct ctcccgcgat ggaagcccgg
    10261 caagcaggac ggtaatcccg tgccggtgtg gtactcagtg ccggtgatct ttgtgctcag
    10321 atgaaattgc cgggtccgga ttcctgcggg gtgatctttt tactcagatg aaattcctgt
    10381 cgaggaactt ccggaagttt tcaatatact gttcggtgac cgggatgcgg acctcgccga
    10441 atactatgcg gccgcgctct atgaccttca cggtctcaag attgacaata tatgaacggt
    10501 gtacccgcat gaaccggtca ccgggaagcc tggactcaag cgccttcagg gtggatagcg
    10561 aaagaacagg cttcctttca tccttcagcc atatcttgac gtagtccttc agtccttcta
    10621 tataatggat ctcagaaaaa ttgatgcgcc gtatccggct ttctgacctg atgaaaagaa
    10681 agtcattgtt cacctccagt gccggtagct ggctcctctc cgcagctata agcttttctg
    10741 ccttctgaac ggccttcagg aactcggcat agctgaacgg tttgagaagg taatcaaccg
    10801 catcgagcct gaatccctcc agtgcatatt tctcataagc cgtggtgaaa ataacctttg
    10861 gtccgcctgc aatgaccctg gccagctctg ttcccgtcag gtcaggcatc tggatgtcaa
    10921 gcagtaccag atcaacctct gctgactgta tgaatgccac ggcatcgacg ggattgtcga
    10981 atgtcccggc cagctcaagt gttggagtct tttcaacata gcccttcacc agctgtaatg
    11041 cgagtggctc atcgtctatg gctacaactc ttgtcatgaa cattcagtat cagcttctta
    11101 cggtcagttt aactgtatag accgattcct gtcggtcaat tctcaggtca tgacggccgg
    11161 ggtacagtag ggcaagcctc ttccttacgt tgtcaagccc gatgcctgaa gcaacctgcg
    11221 gtccgtcaga agctctgccg ccaatgctgt tggcggcgat gaacgtaatc atcccgtctt
    11281 caaccccgag tgctatatca atgaaggagt tgcccactgc gctgaccccg tgcttgaagg
    11341 cattttcaat gaaaggaata aaaagcagcg gtggcacatc ctgatctgca taatcctcag
    11401 ggaaacttac cgtcagcttc acccggtcac tgagcctgag cttcatcagg tcaatgtaac
    11461 ccttcatgaa ttctatctca ttgcttagcc tggtgttacc ctgttcagac tcatagagca
    11521 tgtatctcat cattttcgag aggcttagca ctgcctcctg tgcatccttc ccgtttatct
    11581 caatgagaga atagatgttg ttcagtgtgt tgaaaaagaa gtgcgggctg acctggttct
    11641 tgaggagcgc cagctccgag ttcagcttct cgcgttccag ctctttaatc tcctcttctt
    11701 tcctgacggc gctctcggcg tacctgatcc ccacggcaaa gccggagaca aggaatgatg
    11761 tgatgaagaa gttgtaaagt gccatacccc ctccccttcg cggcatgcgg ttgtctccct
    11821 gagtcctctg attcgcctcc ctgattcgct ccatcacttc gcgcggcggc gcgaaaagat
    11881 tctcgtaata cttgccggag gcatagccca gaacgacaat aagtgatgct gccagcacca
    11941 ggaaggcgaa ccacttcttc ttctgggcaa ggcggggaac cagccagaaa tagttggtat
    12001 agaaaagaat caggaatacg agggtattga agagtacaac cctggttgta cgcccatcat
    12061 tgaacagccc tcccgagatc agaaactggg gcaggataaa cagtattatc caccccgttg
    12121 catgaagcaa aacgggtagc ccctttctct tttttgccat tgaatcactt ttcataccat
    12181 catcaaagct aatcaatccg gttcaatcat tcaaatcgca gtgcatctat ggggtcaagc
    12241 tcggatgccc ggcgtgcagg gtaccacccg aagaatactc ctatgaccgt gcataccgcg
    12301 aaggccagaa tgaccgaacc ccacatcacg cttatcggcc aggaggtgag cacctcaacg
    12361 atggaggagg ccgcgaaacc aagaactatc ccgatcaggc cgccgaaaac gctcagcagt
    12421 atcgattcaa tgaggaactg aagcaatatg tcaacactgc ggccaccaac cgacatcctg
    12481 agacctattt cccgggtacg ctcagtgact gaaacataca tgatattcat gattcctatc
    12541 cctcccacaa ggagtgatat gccggctata gcgccaagca gtatggtaag gatctccgtc
    12601 accgaggtca ttgtagtggc aagctcctcc tggctgcgga cttcaaagtc gttgtcctcg
    12661 ttctcgcgca gtttatgggt gcggcgaagg acctcctcca cctgagactg cgccaggtct
    12721 gactcctccg cactgcgggc agacatctgg attgactgta tataattctg ggcgagtatc
    12781 cttttctgta cggttgtgta cggagcaatg atcacgtcgt cctggtcctg cccgaaactg
    12841 ttctgaccct tctcttcgag gacaccaata atcttaaagg gtatactctt gaagcgtatt
    12901 gtctctcccg taggatcaac tccttcaccg aagagttcgt cagccaccgt ccgtcccaca
    12961 aggcatacct tagcggagcc ttctgcctca gtgtctgtga tattgcggcc actgacaact
    13021 ttccagctcc tgatctcaag gtactgttcg ctcccgccgt agatagtagt gggccagttc
    13081 gaatttccgt aaacgacctg gccgctgctt cttacctccg gtgagacggc aacaacagca
    13141 tcgcattcca ttttgatggc ttttatgtca gctactgtaa gcctctgcga tgacgagctt
    13201 ccctgctgga cccctcccat cctcatggtt ccgggcatga cgaaaagcat attggagccc
    13261 atgctcgcta tctggtcctg gatgcttttc tttgacccct ggccaattgc aagcattgta
    13321 attacagatg caaccccgat tataattccc agcatcgtca ggaatgagcg cattttattg
    13381 cgcttcaatg cgttgattgc tacttttaaa agatttcgcg gattcatttc tttgtttttt
    13441 tagtcttgct ccacaggaag ggctctgagc agttccgctg catcaagcct gttttttact
    13501 tcctcttctc ttattatatg gccgtcacgg aaaacaacgc tgcgcgtcat gcagcgggct
    13561 atgtcaggct catgggtcac gaacacgatt gttttgcctt tggcgttcag atcctggaac
    13621 aacgacatta tctcatatga ggtccttgta tcaaggttac cggtagcctc atcggcaagg
    13681 atcaccacag ggtcattcac aagtgagcgc gctattgcca ccctctgctg ctgccctccg
    13741 gagagctggt tgggcatatg gcccatcctg tcagccagcc caactgcttc aagtgctgca
    13801 acagcctttt ccctgcgctc tctggcactg atgacacgat tgtacatcag cggaagttca
    13861 acattctcaa gggctgaagt acgcgaaagc agattatacg actgaaatac aaaacccagc
    13921 ttcaggttgc gcattgctgc acgttcattt tttgacattc ctgccacgtt tacgccgtca
    13981 aggaaatagg tgccgtcagt tggcctgtca aggcatccga taatattcag catggttgat
    14041 ttgccggagc cgctggcgcc cattatcgca acaaactccc cctcgtttat cttcaggtcc
    14101 acccctctca gtgcatgcac tgtgacttca cccacgtgat agtccttttt aagggtggtg
    14161 atatctatga ttgtcttcat ggtcagtttc ttgttgcagc gtttgcgccc gtcctggatg
    14221 aagactgagt gtttcttctt cccggaggcg ttggcatgaa aggattcgag cttacttcct
    14281 gcaccttttt ctccttcgcg gatgcgatag tcatcttcgt aacgacctcg gaaccctctt
    14341 ccaatccgga ttttacttca gtattgcttc cgtcattcga cccaagttct accggcacag
    14401 ggtggatccc cttatcatcc ttaacccata ccattgttgg cattttgaat ccttcaggca
    14461 acttgaagcc ttccggcatc tggccgccgg gcatgccctg catgccgaag cctgccccgg
    14521 cgacgccggg cgctcctgaa ccactcatgc cagagggcat cctgccggta cctcccggaa
    14581 tgccggcgcc tcctgacata ccggcaccgc cggacattcc agcgcctcct gacataccgg
    14641 cccctccggc cattccattc tttgaagcca tctccttcca ctgttgtgcc aggaattcag
    14701 catcaggggt gaacttcaca gctttgtaag gaacaatgag cacatccttt acctcctctg
    14761 catatatgac aatattggct gtcatcccgg gcagaagctt ctgatccggg ttgggagcat
    14821 tgataattac cgtatatgta acaacggttg aggttgtcac tggcgagagc cttatctgtt
    14881 caactgttcc tgagaaaatt tcaccggcat aggcgtcaac atcaaactcc acacgctgac
    14941 ctgtcttcac ctggcctatg tccgtctcgt caatatctgc ttcaacctgc atctgtgtaa
    15001 ggtcctgggc tatagtgaag atctcagggg tgttgaagct tgccgccacc gtctgtccct
    15061 catcaaccgc acggttcatt acaactccgt caatgggtga ataaattgtg gcgtacccca
    15121 gttgaacaat ggctttgtca tactgtgcct gggagttctt cagggtggcc accgacctgt
    15181 catagttgta ctttgcctga tcaaaatcag ccttggcaat aaggttcttt tcccagagag
    15241 ccttgctccg ctcatagttc gaagcctggt attccatctc agcctttgaa ttgtcaaggg
    15301 tggccagcgc ctgctgaacg ctcgacctca gagccgtctt gtcaagctcg gccaggatcg
    15361 tgcccttttt tacaggtgag ttaaaatcaa catacagttt ttcaactatg cctgatacct
    15421 gtgtccccac cacaaccgag gtgattgcct caagcgtgcc ggtggcggtg acagtattca
    15481 ctatggaacc tttcgtcacc tttgctgttt cataaatgta tgtactgccc ttcctgcagc
    15541 tatgtacaga aagcagtgtg acggcaactg ctgcaagcag cccgatgctt atgaatgtgt
    15601 tttttctttt catcttatta ttgtttgacg gattttattt tcagaatgta agggcgactc
    15661 ccctgtagaa gtcgattatc ttgtggctgt aaacaagctc atactttgcc tggagcaggt
    15721 tgctttcggc agctgtcagg ttggtcttct gtatcaggaa atcgaccgag ttcattgcac
    15781 ccagcttgaa cttctcctcc gaaaggttgt aagtctcaac tgatgagtca tacctgttca
    15841 aggcggcctg gtagcttatc agagccgact cggcgtcaag gacagcctgt tccacctctt
    15901 tccggagctg gttccttgta ttgagctcat caagctccgc agttgtgatg ccgtattttg
    15961 ccctggaaac ggcggtcttg ttctggttgt tgcggaaaat gggaactgag gctgaaaggc
    16021 caaccgaagg tgagaatccg tttttcagct gtgcgcccat ctctgccgcc tcggtgaacc
    16081 cggtgctcat gccggcgctc agtgacagcg aaggaaggaa tcctgccctg gccatctcaa
    16141 ggtcaatcga ggcattttcg cggttgatcg aggcaatctt tatctggggc tttacctcga
    16201 gggcttcctg gtagactgac accgcatcgg cagcaggtgc cgtggaaata aaggcatcga
    16261 tatccgggcg ggctatctca aacgactcgt caaccgggta ttccataagt tgcatcaggt
    16321 taagctttgc catcttaagg ttattgacgg cagttgccag cgaggactcc tccgatgcaa
    16381 gctgggtctt cacctggaga taatcgctgt tagcgatggc gccaagcgtt agtctctctg
    16441 ccgccagggc cagttcctcg cgggtagctt ccgcctggtt acgggcattt atgacctgtt
    16501 cctctgaata aaggacctgg aggtaggcgc tcattatgtt cagcgaaacg ctttccgttg
    16561 tctggtcagc gctgtactgc aatgatttta aatcgagccc tgcctgcctt acctggtttc
    16621 tcagctgaaa accattaaag agcgtcagtc cgctgctgag cgaggcgttg gtgcgtgatg
    16681 agcccaccaa tgaggtttca tccgaggcac cggtttccag tcgccatcca agattctcac
    16741 tcagtgatgc gctgagggat ggcagcatat tatcttttga ctgctgaagg ctcagttccc
    16801 ccaggctgac agatacgccg gctttcctca cctggatatt cttttccagt gcatacccga
    16861 tgcagtcagt cagtgaccac tgcttcgtct gggcagtgac agcaacggag gaaataatta
    16921 aggccgctgc cattgcaata aatcgtttcg ttctcattct cttcattttg tgaaacatga
    16981 tgagacaaaa ctataatggg caggtgccgg ctgaaaattt tttagaccgg cgggaaataa
    17041 tcaccgacag aaaggaatct tttaccgacg gaacgaaagg tcagaataat caggttcctg
    17101 atccgggttc ggaggccgtg cggattccct cggccacttc gggcaggatg cccatcctga
    17161 catagacagg tctgaccagt cgtttgagct cgggcaggcg tctgtagaat accagcgctc
    17221 ccaggaggca gacagcgcca ccgatgaata ttgtccatgg cgtacccagc acctttgcca
    17281 tccagccggc cagcaggctt ccaaacggcg cagtgcccat cagggccatt gtatagaagc
    17341 tcatgaccct cccccgcttg tcatcgtcgg caatggtctg caggatggtg ttgctggcgg
    17401 ctgtgtgcgc catcatccca agaccggtaa agaagatcag gaacatcgaa atgaaaaacg
    17461 aagagctgag cgagatggca atcaatcctg ccgaaagcaa tgccgtagct gcgggaatca
    17521 gccgtcccag cctgagaacc gtatcgcgcg aagcgaggta aatggccccg agaagggctc
    17581 cggcgcctgc cccgcccatc aggaacccgt acgtgtgtga gtcgccctgc agtatgtcct
    17641 tcgcataaac cggcatcagc acctggtatg aagctcccat aagactcacc agtcccagca
    17701 gcagaatgat atgttttatc ggcgcaaagc cgaagacgta atccatcccc tccttcatct
    17761 cccggaacat cggaacccgc tctttctttt cgcggacctc cgtcactctc atcatcatca
    17821 gcgaagctat gacgaagatg taactcagcg cgttgatgag gaagcaggcg ccttcgccca
    17881 cggaggcgag cacgatcccg gccacggaag gacctatcag tctggcgccg ttgaacatca
    17941 tggagttaag ggctatggca ttgccgatat cctccttgcc gtttaccata ttgattacaa
    18001 aagagtggcg tgccggcatg tcgaatgagt tgatgcatcc cagcgccacg ctcagtacca
    18061 ctatatgcca tatttccagg tttccgctga ggctgagcca tgcaagcaca cccgcctgga
    18121 tcatcgagat tatctgtgtc accagcatca cccggtaacg gctccagcgg tcaatgagaa
    18181 cgccggcaaa gggggccagc agaaaagagg gtatcgagct ggcaaaaccc acaacaccca
    18241 gcagcacctc ggagtcagtc atacggtaga caacccaggg caacgctatc ctctgcatcc
    18301 aggtgccaat cagcgaaata ctctgcccgc tgaaaaacag cctgtaatta cggtgacgga
    18361 gtgaccggaa gatggtattc actcttaatg gtatcgaacc gctctgctta ttccttcgtc
    18421 tcatcaggat tttgcctctg aacaggttgt gcttcaggtt ggtcagaaaa cgtatcttac
    18481 atggaatccg cccggataaa tgctccagct ccattcacca tcccgccggg aggtcattgt
    18541 acccgttccg ggacctgctg tctgacagta tttttcataa tcccttacgg ctttcatggg
    18601 ctgggtccat atctgggctt ctgtaattaa cgtgttgata gcaaagttct caagcccgtc
    18661 catgaatgtc cgtttgtatc ccagccaggt gatgagtccg ctgccgaggt tcaccactcc
    18721 ggcaatggca tgcgttttcc atgaccttcc ctccttttcc ctctcggaca gtgccttcag
    18781 gagagcggca gcctgatccg cagataatga cgcttcgccg gaggcatact cacggtaaga
    18841 tggatacgcc ggatttttcg tcaataccgg agtgataacc tgacccatcg ctccgaggaa
    18901 tgtggttgct gctcccagag ccatatcctg ccgaaggatc ctgtcttctg acgtgaaaca
    18961 gaccacgccc tgaccgacgg tggcggcact gtacccggca agccatgcat accaccatct
    19021 ttcggcgcca gccttggcct taagagctgt cagctgaatt tccggtatgt ctttctgtgc
    19081 gactgctccg gtgtcgtttg cgttatacat gaaggcatcc cggtgtatct ccttaatttt
    19141 cttgccggtc agggtgtcgc gtataatttt ctgccccgag atggaaatgg aattgcataa
    19201 aaagagacca cagataactg cagcggagct acacaacctg ttgcataagc ttgtcatcat
    19261 acatcacctt tttggtttcc ggccaggaaa gcagattcct tcagtcttga cgaaaggaag
    19321 taacttatca gcatcagtat gcaaagtact gataatggca ttgtcattga ccctgtaacc
    19381 ggtgatttga accagtccat gccgaaaatg aatgcaatgc ctgatacctg tcccataagg
    19441 aggaggaatc cgtttgaggt gccctctgac accgggaagg ttacttctgc tccgtactgg
    19501 aatcctatcg gtccggagct gagcaggaag aatccgaaag ctgctgccga cacaagaagc
    19561 aaggtgtagt ttcctgtgaa agtgactccg gcaaggctga ttgttgctcc cgcgagggcg
    19621 agctgaatga aaggtacccg tttcctgaag cggtctgaaa ggatcggcat tatgagtgcg
    19681 ccaaccacac ccccgagaat cattaatcct ccggtgattc ccgcctgctc ggcggagaat
    19741 cctctcgggc tgaggatgtt ttcaatccat gtggtaacgg aattaaatac tcccaggccg
    19801 ataaaaaaga tgagcatgag ccacagaaaa tccctgttgc ggaagaggag cctcattccg
    19861 tcgatagcca gggcgcgttc ctcctggtca gggcggcacg gcggggtgac gggcctctcc
    19921 ctggccagaa taaaaaatat tattgcggtt gccatggata ccatgccgta caacttaagc
    19981 attccgtcaa tgtttctgcc gttcaccagg aacggagtca ggctcattcc caggagtatc
    20041 ccgaaataca tcgccagggt acccagcccc gaggcagtgg ctctctcact gaccggaaac
    20101 cacctggaag ccaccttggt gattgaattc aggagaaacg gctgaccaac ggcaatccct
    20161 atctgaaata taagtaaaag gttatagtca gatgcaaaga tgccgcgtaa cagtccgaac
    20221 acaccggtga gaacaacccc tgcacctaca cctgcacgga tgccataagt gtcgatgatc
    20281 cacgatgccg ggatcgacac aataaggtaa actatcatga agctcatgga gagtaggccg
    20341 atctgcaggt cagtaacacc aaagaatgcc gcggcatcct tggtaatagg tgcaaaagtg
    20401 atccacatga tttgattgac agctatcgca agcatgtaaa ccgacagcat gacccacctg
    20461 taaccgtaaa ccctgaattc agtttccttc ataagaatag attgcctatg accaggtcct
    20521 gtcagcaatg aactgatcag gtacccgaat cagtgtataa aagtaacttt tcagaaatta
    20581 caagggctcc tttctctgag aagaatttaa ttgccgggtt atggttataa cctttccggc
    20641 cttacagaca tccattgaaa tatataactt gtaacgtcaa atgaaatcat gcaactgaag
    20701 tcagttgtaa caagataata ttacagataa ataacttacg tatgttttac attctgtttt
    20761 acaagactgt tgagaactac attgagcgcc gggccccgtt ccgtgaggat cacctcggcc
    20821 tggtgcacga gttccacaat gacggacgca tgttcatggc cggagccctg gccgagccgg
    20881 ctgacggtgc agtgctgatc ttccacggtg acacgccgga ggcagcccat gaattcgtca
    20941 gccgcgatcc ctacgtgaag aacggtctca tcaccgaatg gtccgtgaga ccgtggatgg
    21001 tggtgacagg ttaatgtcaa accaaaatta tcaagccata tgaaaaacaa tcaaagccac
    21061 tccggcatgg caatcaatct taccgggctg ctaatgatga tcctccttgc acctgcattg
    21121 acagcagggg cacaggatcc gttcatgcaa taccctgata ttcagggcaa tattattgtc
    21181 ttcgccagcg gcggagatct ctggaaggtt cccgctgaag gcggaacagc ctgccggctg
    21241 actttcagcg acggccgtga aagcgtgcct gaaatctctc ctgacggcag cctgatagct
    21301 ttcacaggtg aatatgatgg caacgccgac gtctatatca tgaacattga cggtggaaat
    21361 atcacaaggc tcacctggca tcccggaacc gatgaagttg tggggtggaa tgctgtcaaa
    21421 aacaagatca tgttcacctc cggccgcaac agcccctcac gttatgtgaa gatgtacctg
    21481 gtgtctcccg atggcaccgg cctggaggag atgataatgt atgacgccgc acgcggaagt
    21541 ttttcgcctg acggcacaaa gatcgcatat aacaaggatt cgcaggacaa tgccacctgg
    21601 aaacggtaca ggggaggaag ggcacaggag gtttatatct atgatcttga gaccgacaag
    21661 gagaccaata tcagcaacta tgacgggtca gacaggtggc cgatgtggat cggggacaaa
    21721 gtttatttca gctctgaccg cgaccgggta ctgaatatct ggtcatatga tcccggcacc
    21781 gggaagattg aacaggtcac aaaacacacg gaatacgacg tccggcatcc tgacttcggc
    21841 ggaagccaga ttgtctatga acttggaggg gatatctgga aacttgacgt aacctccggc
    21901 gtcacagcac gggttccggt aaagatcctt accgacatgg aggaacggcg tccgttcatg
    21961 aaagatgtca gtcgtaacat cacccgtgcc gacatctcac cctccggtaa tcgtgcactg
    22021 atagtcgcac gcggcgatat attcaccctt cctgaaaagg agggcccggt ccgtaacctg
    22081 accgcctcac ctggcgcccg tgacaaggat gcagtatggt cacccgacgg taaacggata
    22141 gcatacttct ctgacgcctc aggtgaatat gagctctatg tcattgaacc tgacggcaaa
    22201 gagaagccgc tgaagcttac ctccctcggg gccggctacc gccacacgat taaatggtct
    22261 cctgacagca aaaagattgc atggacagac cagacgctca ctctgtggtt tattgacgtg
    22321 gcaacaaagg caattacaaa agttgataag gaagaatatg agaatgtgga tgtttccctt
    22381 gacatcaaat caatctttga ctactcatgg tcacctgaca gccggtttat tgtttattcg
    22441 aagatgaacg aggcattcat gtatcagctt tatgtctacg gacttgaggc aaagagcatc
    22501 aactgcatca gcaacgggct gttccatgat ttcaatcccg tgtttacaaa tgacggagag
    22561 tatatcattt tcatttccaa ccgccggttt gaacccacct attgtgacct ggagtgggag
    22621 atggtatatc agaagattgc aggcctatat gcgataacac ttaagaagga cggcaaatca
    22681 ctgatgccgt tcaaaagcga cgaagagcct ggcgccacaa cagtggtgac ggcacccccg
    22741 gcaatcccag ttgcgggaaa aggttccgct gcagcgaaag gaaacgcagc agggaaaacc
    22801 ccgacagcac aaccggccat gagagcaccg gcggcacagg gacagggttc aaccgttact
    22861 gtacagattg acttcgaggg aatcacagac cggacagaag cccttccgct tgaaaagggc
    22921 aactaccgca gccttgccgt gaatgacagg tctctcttct ggctcaacag cgacgagggt
    22981 aacttcaacc gtttcgaggt agccggacac ggaccgatga acctcttctc atggagtttc
    23041 aaatcagcga aacagtccgc tctgaccgat gggattgacg actaccggct gtctgctgac
    23101 ggaagcacca tcttgctccg gaaagacggc ggggtatcac ttatgcccgc agacggaggc
    23161 aacgcttcgc cactgaaact gaccgacttg aaggtatggt atgacccggt aagcgagtgg
    23221 aaacagatct tcaacgaggc gtggcgtatg gagcgcgact attattatga accggggatg
    23281 cacgggcagg actggccagc aatgaagcag aagtatgaaa aactggccga cagggccaca
    23341 tgccgccagg atctgacctt cataatcggg gagatgattg ccgagctgaa tacctcacat
    23401 acctatgtgt acggaggtga cagcaagcgg cgcgctgatc aggtgaacgt cgggatgctc
    23461 ggggccgatt attcagtcga cagtcagaat aacctttaca ggtttgctaa aatctacagg
    23521 gagaaggact ggtcgcgcga agcatggcct ccgctggcca aacctggcat caacattacc
    23581 gagggcgact accttctcaa ggttaacaat accgagataa aggctgacag ggaggtgtgc
    23641 agttattttg ttggccttgc cggcaagcag gtgacgctga cggtgaactc tagaccgacg
    23701 ctggcgggtg cccgcgaggt aactgttgtg cctgccggaa gcgaaagcag cttccggtac
    23761 atggactggc tcgagtcgaa ccgtaaggct gtggataaag cctcaggcgg aaagataggt
    23821 tacatatacc tgcctgacac ctggaacgga tcagctactg atttccccag gtacttctac
    23881 tcacagacga agaaggaagg gcttgtaatt gacggcaggt tcaacggcgg cggacttgac
    23941 ccggagatct tcctgcagcg cctgcagaag aaaccgcacg cattctggac acgtcggcag
    24001 tcagttgacc agccaattcc gcacctcgct gttgatgcac agatggcgct gataaccaac
    24061 aggtatgccg gctcaggagg tgatgagctg ccctatgagt tccgctggtt cggcatgggt
    24121 ccgataatcg gaacccgcac ctggggcgga ctggtagggg tatcccagtt tatcgagctg
    24181 attgacggag gaacaatcac cgcacccgac taccgcatat acaacgataa gggtgaatgg
    24241 gttgtggaga atgagggcgt cactcctgat atcataattg acgttgactc gaaaaagtac
    24301 tcagagggat atgacactca gctgatgaaa gccgtggagg tggtgatgaa gaagatctcg
    24361 gaagaaccgc gccagtggcc acagcataag ccatacccgg ttgacaggta gctgcttctg
    24421 gctgaattgc cgggtttgac cagattccta cgattaatta aaaccagata aattgaggag
    24481 gtgcatcacc ggcgcctcct catctttttc ctacagcatc aatcagatac ctggccgcct
    24541 gatctcccgg gaacctgtcg gcaagctgac gggcatattt caatgcctta tccttcatgc
    24601 catgctccag gcagaaggta gccattgcat agaggtaatc atagttgccg ggctcccggt
    24661 taagtgcttt taacagatac tcttcggccc gggcggtgtt tcccagggta ttctcgagca
    24721 acccgaggtt gtaaagtatc ctcgtctgct caggcatcag ccttgctgcc ttcaggaaaa
    24781 actccctgct ctcctcgtac ctgccctgct cggccagcag catcgccagt gagctgttga
    24841 gctcatgcag acccgggtga tccctgagaa gttcgcggag cagcttttcc gcctcggccg
    24901 tcctcccctg ctgtgcacag acagttgcaa ggttcacctt tgccatgtaa aactgatcgt
    24961 caacgccgag ggcctcgcgg taagcggctt ctgccttcgg aaaatcaccg gcattggcat
    25021 acatgatacc caggttaaac aaccctccgg ggaagtcggc attgtaaaga ttgatctccc
    25081 ggtattcatc cagcgcggcc ctcctcgctt tgcgggtagc aggactcagc tgatcctccc
    25141 tcacctcgga gagcctgatg gcagcctctg cccttatccc cctgaccgga tcattcagca
    25201 gaggcatcat cagcttctca tatgatgcga tgttctccgg gttatagctc atcacagccg
    25261 tatgtctcac cagcgaggcg ggatcggtta acgcccgctc cacggcacgg ttactctcag
    25321 gggtgttgaa ccgtccgagg aacctgactg ctgtcgccct caccatcagc gggaagagct
    25381 cattctccga atagagtacc agatcagata tcacgctccg gtcacctttt tccgctgccg
    25441 cgagagtctc accatagtgg ggcctcgtgc gctcaccgta ccatttcagg atgtaactct
    25501 gcgaccatgc tgtgctttta tccttatggc agtcattgca ggcgttagga acgccggttt
    25561 tagccgtaag gtcaggacgg gggatcctga agctgtggtc acgacgatag tcattgccca
    25621 tataatacct gccggtcata tggcagttta cgcactgggc accggtaccc tcgacatatt
    25681 tcggttctcc gcgattgatg agacgtcccg tgccggtact ctcccggtat agctgcgttc
    25741 ccttgcctgc cggctgccct ccggcgacgt tcccgctgta aacgtttgcc ttactcataa
    25801 gctgtccgcc ggcaacagcc ggatccatct tatgaaaatg atgtctcggt gtgtcataga
    25861 tatccggacg gtgacactgg aggcaaagac ggttatcagg ttccttcgtc cttgcagaat
    25921 gcgagtcatg acagtcgctg cacatcacat cctgctcaaa catcctgctt tgtgtgaatg
    25981 agccatactc atagacttcg tcgagtatct gcccgtcaac atgatacaga ggctgtgccg
    26041 tcagcatcgg gatcatgtag ttgagcaggt cattgttgtc atctgcatag tttcccatta
    26101 tcgccctgcg tgagtggcac cgtgcgcaca cgtttaacag ctcttcgttg ttcaggtccc
    26161 gggtccggac aatcagtccc gtattgacat ccgccggcct cgagccttcg ggaagggcag
    26221 cccataccag gtgctccgag gcaggaccgt ggcatgcctc gcagctgaca tcgatctctg
    26281 accaggtggt gttgtatgtc tttgtggccg ggttgtagtt cttcttcaga ttggtcgagt
    26341 ggcagtcggc gcacatgccg ttccagttct gagcctgccg tgtccagtgc agccagttgc
    26401 cgggacccac ctcctcatca gtgtatacgg tgtcgcccag gtggaaccat ctcccccgct
    26461 ccgtatccca tgcgatcggc aggcactgca aacgtcctcc ttcgaacggc acgaggtact
    26521 gctgcagggg agtatatccg aaggtgtagg cgacttcgaa atcttcgaag cggccggtgg
    26581 cgccttcagt gtttacgaag tacttttcgc ccttgcggaa gaagcggtgc acctctcccc
    26641 ggtgcctgaa ctcatagttg ttgaaatcgc ccagcacgct gctgtcggtg gcgatgtcca
    26701 tcgccaggtc atggtgcgat cccttccaga ggtcatactc ccttttgtgg cattcgatgc
    26761 aggtggcagc gccggtgaaa gagggacctt gctcccgctc ctccccctgt ttctcaagtg
    26821 ctgtccgtac caggtagacc gggaaggaaa ccaccagcac cacagtggca atgaagccga
    26881 tcctcttcca gctcttcata ctgtcagtgc tgccagtctc tttcatttcc ctggcgctca
    26941 tcccgtattt gtgatcatta aagagcatag gtcagctggt tggattcaaa cctctttcgt
    27001 ttcgtcatgc tgcccaaatc gactttcacc ttgccctcct ctatgatgac gggaaggtag
    27061 tcgagtggcc tgggagcggg tgagctgaga acgatgcccc tgtggtcaaa tgccgatgag
    27121 tggcatgggc agtcgaactg ctgtgtctcc gcattccaca gcaccgaaca gccgagatgc
    27181 gggcaggtca gcgagagagc caggaaagcg ccatcttttt cccggacgat atagagcctg
    27241 ttgaccctgt caggggtgac tgatcctgcc gggaattcct ctacgctgcc aatcacacgg
    27301 atagtgtccg ccggcctgct cttatgggtt tcctttgatg gtttgagaag gctgaggata
    27361 aaccacagga attcagccag tgcaacgatg ccaagtatct tccagagcct ccttgtaaag
    27421 ttccgccttc ctttccggtg ttctgatcct gtatctttta ttctgccact catctcctga
    27481 ctttatcctt tcataatcac acgttccacg gccacacaag gttcatcccc ccacccctga
    27541 agaatatccc ggtaacggta agcacagcat aggatacaac catcagtacc acaatcgatt
    27601 gtatcatctc ggagcgtttg agtgagtagc gtctgcggag atatttcagt aagagccata
    27661 ctgttccggc tgccaggatg aacggaacaa gccccgtggt aagcagcggt gtcacttctg
    27721 gcagaagaac ctcggggtca gggagtatct cactcatggt gatgaagatg attgccatta
    27781 tcatacctgt tgcagccgcc attcttgccg atctgattcc ggcaggcgac aggaaccaga
    27841 cgccctggtt tctatcagtg cttttaatgt atggcatcca tacagctgca accagtgccg
    27901 tcacaggcag tatgaagact gcaaacagcg ggtgaaaatg aatcagcagt tcctgaagac
    27961 ccatgaagta ccatggtgct ttggcaggat tgggactgaa ggcagggttt gccctctcac
    28021 gcaggggggc gtcgagaagg ctgctgatta taaatattgc tgcaagcaga gtgatcgcca
    28081 ccacaaactc cctgttgaca agatacggtt ttgtatctgc catcgggctc tctttatctc
    28141 gatcagccac gatgacaccc ccggcacgac gaatcctcca gaagtgccac gccatcaggg
    28201 cgataatcat tagcgggatg accccggtat gcaggttaaa gaagttggtg agggtgggct
    28261 gccctacctc cgctccgcca agtaatgctc tgcggattgg ctctccggcg agcgggatgt
    28321 atgacagcag gctggtgccc acggtcaccg cccagtatga cagctggtcc catggcagga
    28381 ggtagccggt gaaatttgag agcaccacaa gcagcatcaa tgccagtccg gttatccagg
    28441 tcccgtctct cggcgggcgg taggcaccgg taaaggcagt gcggagcata tggagaaatg
    28501 ccacccagac aaaggctatt gcgctccagt ggtggatatt gcggagcatc tttccgaaga
    28561 ggaggctctc ctgcaggttc agtatcgagt tatatgcatt ctccggtgag ggttcatagt
    28621 gaaacttgag cagtagtccg gttaccacct gtatcacaac aagcagcgca gccatcccgc
    28681 cgaggccgaa tgtaagggtg aagcggagtg ttgatgcctt caccttccgc ggatgcaggt
    28741 gaaggatgag tccgaaccgc gatttctctc tcttttcagt ggcgtgatgc tctgtcatca
    28801 ggatttccgg ctcgatgcct taaaaacaaa gataaattat ttcactatct ttgccccgcc
    28861 cgggacgtta gctcagtcgg tttagtccgc cagccggcgg atcacacgca agaggctttg
    28921 atataatggg acgttagctc agtcggttta gtccgccagc cggcggatca cacgcaagag
    28981 gctttgatat aatgggacgt tagctcagtc ggtttagagc gcttgcttca cacgcaagag
    29041 gtcactggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg gacgttagct
    29161 cagtcggttt agagcgcttg cttcacacgc aagaggtcac tggttcgaat ccagtacgtc
    29221 ccacacagaa tgttccactt tggttcacat taatcctggt aaatgtgttc agaattcagg
    29281 aaggaaacca atgtttctta aactcccagg gaagccagtg aaataacatt aataccatcg
    29341 ggacgagtgt gacttactcc ggtgccgaca atgatgttca atgattttgg gggacctatg
    29401 ctttttatgt ccaatacctt tattagtttt ttaaggttgg aagcagcttc attaatcatg
    29461 cccataccga gctttatctc aaaggctgcc caatcaccgt tgcgtttctg tataatgcag
    29521 tcgatctcaa ggccactcga atcagagtaa tggcagacgc aggcatcatt tgccctggca
    29581 taaacccgca tatcatgggt gaccagcgat tcgaacagga agcctgaaaa cttcacatct
    29641 cttaaaagtg attccttatc agcacccaat gcagctacag acagcgagac atctgtaaaa
    29701 tgcattttcg gtgattttct cagcgatgca gaggagcgga tatgtgtact ccatgccggt
    29761 tggttttcaa tgatcattaa tcgttccagg gcatcgagat attcatttac agttggacgc
    29821 gagacatctc ctttgtcatt ctctttaata tccttaacga tagtggtttt tgcaagctgt
    29881 tttgtttatt tattaatcaa tcccggatga attcaactca atccgtgggg attactggaa
    29941 attcttttct atttttcaca ggtgtgaccg gttgtatgtc atccggcaga aatatcagac
    30001 cagaagaatg atgaataaaa accctctatc cagcgccatc accgggaaca tgaatagtcc
    30061 caggcttctt tgcagccgga tcaccctgac gttgctcttc ctgttgccgc tcctgtctgc
    30121 atcagccgaa tacagaggtg ttgtatataa tgacctgaac aggaacagac aacacgatgc
    30181 cggcgaaaaa ggaatgagcg gagtggctgt gtcagacgga ctgaatgtgg tccttaccga
    30241 ccgccagggc gccttccggc tccccggaac accggaaacg aggttcatcc gcataaccgt
    30301 tccggcagga tacatatcct caggcagctt ttaccggaaa gccgacggac aggagtcagg
    30361 atatgatttc gggcttgtcc ctttcaacgc ttcgggaaat aaaacagtcc gttttctgca
    30421 actcgccgac actgaaaccg gttatgactt cggatggatt gcgccaatca gggattatgc
    30481 tgcaaatgaa gaggtcagct ttattatcca taccggtgat atctgctatg aatacgggct
    30541 gaactttcac ggaagcaatg tcatggctga aacgatgggt gtccctgttt actattgtgt
    30601 cgggaatcac gacctggtaa agggcaatta tggcgaagag cttttcgaga agaatttcgg
    30661 gccggcattt tattctttcg atgcagggaa cacccatttc atcatcaccc ctatgctttc
    30721 cggggactat aagccttcct atacaaagga ggacgtctac aggtggatga aaaatgacct
    30781 ccggttggtt gacccttcaa aaaatcttgt agtctttaac cataaccttc tcaccatggg
    30841 cgataagttc atctacggga tcaatgaggc ggaacatatc aacctcggcg aacacaacct
    30901 gaaggcctgg atttacgggc actggcactc gaattacttc atacgtcacg gtaacagcgg
    30961 cattgtctca ttctgcagtg cccctccgaa caaaggaggg atagaccact ccgtttcaaa
    31021 ttttccggtg tatgaaattg acggtgaagg caatatagcg gctcgtccca ggtacaatta
    31081 ccttaacaat cactttgcag cggtgagccc ttcaggcaac caggcagtta tcgatgacag
    31141 tggtaagctt ttagtttcgg tgaacaccta ttcaaccgtg tccccgacgg atgcagcaga
    31201 gtttatgata gagggtttgg gagaatggaa ggcactcagc cgggaatctg actggaactg
    31261 gtcaggaaaa tccgatgcag aggatatgaa gaggggcatt ccctaccata tcaatttcag
    31321 ggtacggctt aagaacggag ataccttttc atcagtgcag aactttatat tttcagaaga
    31381 tgcttcaatc gaccaaagcg cagaagtcac gctgaaagat cctttgaaac tgaagtggat
    31441 aacaaatgcc ggtacaggat ccggaatctg tccacctgtt tactcggacg gaacacttta
    31501 tgttgctgtt tacgaagatt atggcaccaa caataaaata atggctctcg acggttcaac
    31561 gggtgatctg aaatggcagt ttttgaccga aaatcctgta aaaaacacac tgtcattatc
    31621 tgacggtcat ttatttgcga ccgatgaggg ggcgattgca tattcaataa atgccggtaa
    31681 cggcgaactt gcctggaaaa ggaaccttgg cctcagcacc tttcccctgc cggttaacgg
    31741 tggcgtagtt cacaatggcg tttactattg tggatatgga aattaccttt ctgcacttga
    31801 cagccatgac ggccacctga tatggaagaa tgacagctgg aacggaggag aaggcacaac
    31861 atccaccatg gctgttcatg gcaatacact cattgccggg gccaactggc gggcacttta
    31921 cggccatgac cttcagtccg gggcaaaaaa atgggaggtt agcagtgacg gcatacgctt
    31981 ctgtaacggc acggccgctt ttattgatga tactatgttt tttgcggccg agagagccct
    32041 gatccggatg gaccccggaa ccgggtcaat atacaaagtt aacccggtcc cctatgacat
    32101 gcaggttgct tccaccccgc tggtgactga caaaattatt gtaacaggaa cctcagctga
    32161 agggctggtt gcctgggacc gttattcgtc aaaggagctc tggaaggtga agccggtcac
    32221 atcactttta tataccgccc cttattcaaa accgccggcc tccacggttg aaacctcgcc
    32281 catagctgtg ggagacatga tagtcttcgg tgcctctgac ggttacctgt acgccattga
    32341 cagtggcaac ggaaggatca ggtgcaaaat aaacctgggg gctcctgtct tcagcacagc
    32401 ctgcctggct ggcgaccttc tgtatgtggc tgatttctca ggaaatattt cctgctttta
    32461 cttgaaaaaa taacctgatc attgcttccc tgcgggtaag cagaaaatat ccggtctggt
    32521 acgttcccac cccaagggat aaaaagaaga ggccacaaca ttcacctgat gggtgaatgt
    32581 gatggccttt cacagaacat cagaaagatc taaatcttac tgcttcacca gcgaaccgcc
    32641 cacatatttt actgtaaggc tacccgtggc attggggagc acgaattgct ttgatgtaag
    32701 tgtctgcccg ttttgaccgg cataaatcgt aaacgtaatt tccggaaagt tttccgtatc
    32761 ctttacatcc cagacatagt tacgcaattt tcccggagca agcttgtttg acggatcaat
    32821 ggactcaccg tttgtccaca tatgaacgtc ttcagatgag ccattaatca tagtgacagt
    32881 gatcatattc tcggtttcga tatcgcatcc gggaataaca gacagtaaca ctaatagtgc
    32941 aaacgcggta atcagggcgt atacgctttt cctttgcatt ataacctcct ttatgtttta
    33001 gttgtttgta cttctaaaac gtacttcagg cagcagatat tcattcccgt gtcatgcttt
    33061 acaacatctc cgccttcacc ttgtcagaac tgttcaggct gatcacccat ccctttcatc
    33121 ccgggttttt ctgttgaaga taaaatatac aggtattcct gccaggatga gtgacagacc
    33181 aatcattgcc tcccttggcc gggtgattat agtattgaaa aagagtccga tgcagaaaag
    33241 tacaaagatt gccgtcacaa caggatatcc ccagaccctg tagggccggt gagcataggg
    33301 catgcggcgg cgcaagatga acactcctgt tgccatcgca ccgtagaata tatagacggc
    33361 aaagatcagc atgtctgtca gctggtcgaa tgtacctgac agcaccagta ccgatgccca
    33421 tatcccctgc cacagcagcg agttgccggg cacgttgaag cggttgagct tcccgatgcc
    33481 tgagaagaag agcctctcct gcgacattgc ataatacggc cgtgatcccg tgaggatgct
    33541 tgcgttcagg catcccatcg ttgtgatcag tatcagaacg gagataaaga gcaccccgcc
    33601 ttgtccccac agactcctga cagcctctat tgcagcgatc tggttccccg cctcgtgaac
    33661 ctgtacaagc tccgggatcg acattatcct catataggcc aggttgacaa ggacatacac
    33721 tgcaatcacc acaagcacgc cgatggcgat gcccttcggg atattgttgt tggcattcct
    33781 gatctctcct cctatgaacc ctactgacac ccacccctgg taagcccaga atgcagcaag
    33841 catcgcggta tagaatgagg atagagtcac agtgccctcg ccaagttccc tgaaattcat
    33901 aaagttttcc ggtcttggat tgttcgtggt gaagccggca atcacaatca ccagcagtcc
    33961 ggcacagaca aaaaagaaga ttgccttccc tgtccaggcc ccgctgcgaa gtcctgacat
    34021 gttgagcagt gtcagcagca tgatgacgat gattgctgcc agctttatgc cgaaatcctg
    34081 aaacgggaag aacaccccgc ctattgaaaa atcatgcagc gcaggaagca ggtcgggaat
    34141 cgggatgacg ctgttcagcg actgcaccag cacatacgcc agggaagaaa tcgttgcagt
    34201 ctggataact gcaaaaagtg accagccgta aaggaatgag aagaatcgtt tatagatctt
    34261 tttcagataa acatagtcgc ccccggtatc ggcaagcatt ccggcaagtt cggcattgct
    34321 cagcgcgccg aagagggtta tgagtccgcc tgctatccat acgaggagga tccagaccga
    34381 ggattgcagc tcatcggcca tgggcgctat cttcttatat acgcctgatc ctataatatt
    34441 ggcaatcaca aatatgacca cgtacccaag cccgagtgtc ctggataaac ttctctgatc
    34501 agtcatgagc tggcatagtt ctgagggtga agtcgctaat ttaagaaata agtactgaga
    34561 attgtgaggt gtgatttgcg atttgcgaag cctgaggagc aggttgtaag gtgaaatcca
    34621 taatttttgc attcgcgcaa gtggtatgcc agcgagacgc aagaccatct tgcgacaaaa
    34681 cattcctgac acttatgcgt ataaagtacg taacaatctt cactccgaaa aatgaaaaaa
    34741 ccggtcatta cccttctggc aatgcttctc tgcactggcg ctaccgccca gtggaaggcc
    34801 cagaccgccg atacatacga aaaaacctac aggatggcat ttgttgtctc cgcaagcggg
    34861 accgaaactt tgaggatcat gcgcgacgtg acaccaggca cctcaaccaa aggtgcaaat
    34921 ccttatgatc agatttccgg acagattctg cttaataaaa atgtaggcac tgaatactcg
    34981 gttaacacta ttgttttaag gttcgacgaa agcacaaagc tttatataca tcagcctgct
    35041 gaccttaagc agggctggga cgccaacgcc ggaagatata taattgaatc agactggcag
    35101 gtctggcgaa cgggcgatac ccgtaacaag ggggccggca gggctgcagc aggctccggc
    35161 acaaatctta aaaaagagat tcttgacatg atgaagtcgg gcaaaacggt ccattgtcag
    35221 acaattctga ttcataagat gcatgatacc cagaccatta tcaactcgga atttaaactg
    35281 cagaactcga ccaggtcgat caactacctg tttcaataat ctgcccgata acatttcctt
    35341 atcttcagaa attttgttct tttgaaggag agatccttaa ctgaacgaaa tcaatgaaaa
    35401 agattaccct cgtactcttt gtgatggcga tgattattgg ctatcatccg gacctttttg
    35461 cacagaagaa aaacaggaaa gctgaatcgg cggctcagcc ggcggcagac atgaagcccg
    35521 aggcacagaa gcaggtgcag gcagctactg tggttccggt aatagtaaat ggtgaagcgc
    35581 agaagatacc tgcatttgaa aatgccggcg actggatccg cgaggatctg tgggttgaga
    35641 cggagtttga cactgacggc gacggtaaga atgacaggat gcatgttgat gtcacacgcc
    35701 cgaagcagac tgacactgaa gggataaagc ttcccgtggt gtatgagtcg agtccatact
    35761 tcgcaggcac tgccggcaac aataaggaat atttctggaa tgtaaggcag gaactcaata
    35821 ccgttcccaa accacatatt tatcccccgc agatcgaaag gagaggtgag cggcctgtta
    35881 tttccaactc acaggtaaag gcatggctgc cctacggttt tgccgtggtt cattcatctg
    35941 cgcccggaac aggactttcg cagggatgcc ccacaatagg tacgcggata gaggccctgg
    36001 cgccgaaggc tgtgatagac tggctcaacg gacgggccaa aggatacacc acacctgacg
    36061 gaagcgttga ggtaaaggca tactgggcaa ccggcaaggt aggaatgata ggcacatcgt
    36121 acaacggaac aatccccttt gccgctgcca caacaggagt tgaagggctt gaagccatta
    36181 tacctgtagc gcccaataca tcatactatc actattaccg ctccaacgga ctggtcagaa
    36241 gcccgggagg atacctgggt gaagatgccg atgtcctgta tgattttgtc ttctccaatc
    36301 ctgataactg tgactactgt gacagtgtgg taagggacgg ggagatggct gccggacttg
    36361 accgtatcac cggtgactac aatgagttct gggccaaacg tgacctgatg aactatatga
    36421 agccctataa ggcagccacc ctgatggcgc atgccttcaa cgactggaac gttgtgccca
    36481 gtcacagtta ccgtttcatt gaaaagctga aggagatggg agtgccgctg cagatatact
    36541 atcaccaggg aagccatggc ggtgagcctc cgttcaaaat gatgaacagg tggttcaccc
    36601 gttacctttt cggaattgaa aacggggtgg agaatgatcc gaaagcatgg atcgtccgcg
    36661 aaggagccga acggctcagc cctacagcat atactgatta tcccaatccg gatgccaggc
    36721 ccgttactct gtatctcacg gggggcgccc cggaggcagg cggactggtg atgacgaagc
    36781 cggcaaaaca ggagaaggaa acccttatcg ataattattc ttttaccggc tcttctcttg
    36841 cccaggctga atacagcaac caccgtctcc tgtacctgac tcccgttctg acggaaccgg
    36901 ttcacatctc gggtgtggtt aagataaaga tcagggcggc ttccagcaaa cctgctgtga
    36961 acctctccgt gtggatggtg gcattgccct gggacgacgg gcgcatcatg aggatgaccg
    37021 aaagcatcat cacccgcggc tgggccgacc tgcagaacta tcgctccctg accgagagtg
    37081 agccgctggt gcctggcaaa ttttatgaga tgagcttcgg gctggagccc gatgaccaga
    37141 tcattcctgc cggaaaacag ataggcctca tgatcttctc aagtgacaag gagttcaccc
    37201 ttcatcctat tcccggaacg gagctgaccg ttgatcttgc cggaacggag gtgacaatcc
    37261 cggtagtagg cggtaagatt aattaagcga cccgtccctt tgcattcagg ttaaggccgg
    37321 tcacttacaa tgaacggcat cagagactgc tgcccgcatg cacaggcaga aatttttcta
    37381 tctatgtatt gcagcgttcc ggcgtgtcat ctcgtattag gtaataaaag acctgagttt
    37441 cgtagaacca ttgcttaaaa acatctgtat gaaaaatcaa gtatttataa ttctgtgcct
    37501 cctgaccttt gccgtattgt catcatgttc aaaggataat ggagaggaac ccgatctcac
    37561 cccggaaaac ctcacactga cagccaagag ttccgaagtc attgcctaca gcaacgaatt
    37621 tggtgttgat ctctttacaa aagttgctct tgccgaaaac aaaaacttca tgctctcacc
    37681 actgagcgca agtgccgcac tgaccatgct gctcaatggt tgtgcggaag acacatatga
    37741 tcagctgaag gctacgctga aatatcctga acagatgacc atcagcgaaa ttaacgaagt
    37801 atacaaaaac cttgtttccc agttgctggt tattgatccg aaggtaaaac tggccctggc
    37861 aaacgcaatt ttctacaggc agggattcac cgtcaagccc ccgttcctca gtacgatgga
    37921 caatgatttc aatgcggaaa ttgccggtct tgatttttct ctgccatcag cgctgacaac
    37981 cattaacaaa tgggccagcg acaatacaaa cggtaaaatc ccggaggtgc tgaatgagat
    38041 ttctcctgaa gctgtgatgt tcatcatgaa tgccctttac ttcaagggcg actggagcta
    38101 ccagttcgac aaggacctga catctgacag ggcattttac actgatggat caaactctgt
    38161 caatgtaagc accatgaagg gggaagtcgg gtcaaaagtg accggcggag caggatacag
    38221 tgcaatcgaa ctcccctatg g
//
LOCUS       DAOPXU010000015        38124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_952_length_38124_cov_11.357482, whole genome shotgun sequence.
ACCESSION   DAOPXU010000015 DAOPXU010000000
VERSION     DAOPXU010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 38124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38124
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_952_length_38124_cov_11.357482"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PKK03_02610"
     CDS             complement(<1..598)
                     /locus_tag="PKK03_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNX83314.1"
                     /translation="MKLIKTMFVLLLAVSFVSCGSGKEKKNAEKKSTEAEVWTYLFDG
                     SGTQGWRNYNKPEFPTQGWEIVDGTLHCIGSGRGEAGGGGGDIIYDKKFSNFHLKMEW
                     KISEGGNSGIFYLAQEGPEWDYIYKTAPEMQVLDNERHPDAQAGKDGNRQAGSLYDLI
                     PAKPQNAKPAGEWNTAEIKVYKGSVWHIQNGETVLEYHL"
     gene            complement(636..2135)
                     /locus_tag="PKK03_02615"
     CDS             complement(636..2135)
                     /locus_tag="PKK03_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX83315.1"
                     /translation="MKEETRKFTRRSFVGTTGAAIAGLTILPSNVVSGLGHRVPSDKL
                     NIAGIGIGGMGATNLRNVAASENIVALCDVDWGYSKRVFDAYPAAKRFWDYRKMYDEM
                     GKSIDAVIIATADHTHALTAAHAMTMGKHVFLQKPLTHSVYESRLLTKLAAKHKVATS
                     MGNQGSSAIGVDLVQEWIQNREIGEVTKVEAFTDRPIWPQGLNRPSKVDKVPKTLDWD
                     LFLGPAPVRPYNAIYHPWNWRGWWDFGTGALGDMACHILHPVFKSLKLTYPTKVQASS
                     TLLLTDCAPNAQTVKYTYPARKAPSHYKIDLPEVEVTWYDGGIQPMKPEGWPIGKDMN
                     DQGGGVIFHGTKDKLICGCYGINPWLLSGRVPNAPETERRVKNATSGGHEMDWVRACK
                     ENPDNRILTKSDFSEAGPFNEMVVMGVLAVRLQGLNKILEWNGEKMEFANISDEETIK
                     ICVEDHFSITDGHPTFDRKFTDPINARQFAAEMIRHTYRSGWSLPEMPA"
     gene            2230..3432
                     /locus_tag="PKK03_02620"
     CDS             2230..3432
                     /locus_tag="PKK03_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HNX83316.1"
                     /translation="MRLRRGLTTLIIANFGILSSYGQYFTRLGIGLDTRTNVSIPVAV
                     ADPNDPQFSGELLAAKYPGLNIPPGKIDLVYPVIPDAADTVAILWYLMPEEISAVPGE
                     MNVIIVEITPGNEKRFWIDNNNDRVFSSSERSFIFRNTEESRPVNIMVAGSYYSYTLF
                     NPDYSPPSAPSETIRQSKSSWSDRKGKPSFAIDLSASFLRADASLSYIKQSRPSNTIT
                     YSARIPGSFRPAIGFDFSWYDFHLLLGGGFEKAKYTENILVSTNDGSQTTSYNSGTWP
                     TSRFILSIALEYDISAGRYLYLTPFAVWSNFKNDSRFNFDRYQGSSAGAEYTDSKSYE
                     AGLKLKMPVDRQVIIHLSLAYSSVYFNAEKHFTDVLPGSYRMSMEGFYYGVGVNFSLR
                     GSAGQDAE"
     gene            3472..3966
                     /locus_tag="PKK03_02625"
     CDS             3472..3966
                     /locus_tag="PKK03_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83317.1"
                     /translation="MKSQRFNLDPGENDRINRILKALFGLVCIAAAITTGIMMTGSDK
                     LTTNSFIAIAFMGLIGISLLISGLGLTERFLTLTGDTIILRDKVYAPARTISVSDLRK
                     VEFSQLKVAFYLKSEEVISLRLGTFYRESPVRIMEALEKFCASNGVLTEGLPSNRDDD
                     GHEN"
     gene            3956..5104
                     /locus_tag="PKK03_02630"
     CDS             3956..5104
                     /locus_tag="PKK03_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX83318.1"
                     /translation="MKIRYGMIGGGEGSLIGPVHRMAARLDGICELVSGSFSSNPEIS
                     RLTGTREALNEARIYDRWQEMITTESTLPEEVRPHFITIVTPNHLHYSPAKMALEAGF
                     HVICDKPLCLSVSEALDLHETVERTGKLFCLTHNYTGYPMVKAARNMVLAGEIGKVRK
                     VIVQYLQGWLASPLERIGNRQAEWRSDPARAGIAGAMADIGTHAFNLAEYVTGEEVVS
                     LSAKLNSAVPGRILDDDGTVSLTFGNNISGTLLASQAATGEENNLSIRIYGESGALIW
                     RQMEPNTLTRLRPDAPAQVIRTGASFAGSGLPASMHTRLPAGHPEGFIEAFGNLYRNF
                     ALHIIAVNDGQKPDPVADYPGINEGVRGMKFLEAVVSSSRNGSVTIKL"
     gene            complement(5183..6034)
                     /locus_tag="PKK03_02635"
     CDS             complement(5183..6034)
                     /locus_tag="PKK03_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNX83319.1"
                     /translation="MTTRRDFLRKSALVIAAAPMINSEMIASSRPSKKTGLALYTIRD
                     AMVKDPAAALAGAAETGYTWIEAADHRDGKFYGMKPKEFSKLVRKNGMVAISSHSGLN
                     PENYEKMIDEAAEAGMKYIILPSLPGDWSNTIDGYQRAADFFNRAGAKCRKAGITFGF
                     HNHQVEFREINGRVPYDILVEQSDPKLVIFEMDLAWISAAGKDPVAYFRKYPGRFEVW
                     HLKDLTPEKQDATLGEGIIDFRPILAEARTAGMKYWFLEQDFCRTHTPMESIRISRDY
                     YLGLLKK"
     gene            6138..7145
                     /gene="amrS"
                     /locus_tag="PKK03_02640"
     CDS             6138..7145
                     /gene="amrS"
                     /locus_tag="PKK03_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNX83320.1"
                     /translation="MQMRLWEKHKEGSVRCTLCPHYCIIRPGERGICGTRENREGEIY
                     PVTAGIISGYALDPIEKKPLYHFHPGSVILSVGSYGCNLKCDFCQNYHISQNVKGEQS
                     ARLDPEELVRQAVTARGSIGIAYTYNEPAIWFEYVTESARLAREKGLRNVMVTNGYVN
                     QEPLKELTQVIDAFNIDLKAFSNDFYRNYTGATLKPVLQAIKTVAGSGRHLEITTLVL
                     PGMNDSPDEMEREAEWISGNAGRSVPLHLSRYFPTYRRTTPATPPETILRLREIAEKY
                     LDFVYTGNMPGEEGGSDTRCPDCHTTVIRRSGYYARISGLTDEGSCVKCSRQIIAASD
                     V"
     gene            complement(7181..7468)
                     /locus_tag="PKK03_02645"
     CDS             complement(7181..7468)
                     /locus_tag="PKK03_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83321.1"
                     /translation="MASIKSLKKDIDFLFFELISDCFMASSLHDGAKKMEVQNLIEEA
                     VVMRNEFIDRANHPDGKDNPALVRAYYADLRKELFGKINEFFEKLSSITKQ"
     gene            complement(7498..8334)
                     /locus_tag="PKK03_02650"
     CDS             complement(7498..8334)
                     /locus_tag="PKK03_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNX83322.1"
                     /translation="MDCTGSYIWLDGAIVPASGNEEIPSGEVTFYEVVRTRDGVPLFF
                     SDHMNRLKDGIATRYDLREDIAAEVRAGMKALANHERHDEINVRVTVSFSGYDHSLHI
                     CYIPSFYPSETMVREGVPLILHHAERLDPGVKMLNNRLRLSVNGELARRNAYEALLVN
                     HDGFITEGSRSNIFFIAEDGTIQTAPDSMVLSGITRRYVTDIILKEGITLVTEAVKES
                     DAGRFRSAFITGTSPMVLAVRSIEGLQFNVADPLIGRLREAYSDLAGRSMNDYKMGDE
                     AD"
     gene            8414..9778
                     /locus_tag="PKK03_02655"
     CDS             8414..9778
                     /locus_tag="PKK03_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNX83323.1"
                     /translation="MYAIVDIETTGGSARLERITEIAVYIHDGNRVVEEYSTLINPER
                     NIPYFITSLTGITNEMVEDAPKFYEVARRIVELTEGKVFVAHNARFDYSFIRQEFGML
                     GYNFKRPMLDTVALSRKLIPGHRSYSLGNLCNDLGIEINGRHRASGDALATVRLLELL
                     LEKDRELNSGSLIKNRKAAKLHPALDMTKLEEIPEEPGVYYFHDEGGDVIYVGKSRNL
                     SERVSSHISNNTSAREMEMRSMIADITWERTGSELIALLMESAEIKSRKPRFNRAQRR
                     TGFRWGIYSETDEAGYIRFSYRNVRDEEVPLAVFTSRDRARGKLEQIITDYNLCQKLC
                     GLYETSGPCFHRQVSMCRGACCGEEDPKQYNERALMALDEFIFRERNFFIIDRGRDEQ
                     ERSAVKIVNGKFAGYGFFSINDVGFGLTAVHDCIKPANDNRDIQVIIKGYLKKNRVEK
                     IIDF"
     gene            9837..10487
                     /locus_tag="PKK03_02660"
     CDS             9837..10487
                     /locus_tag="PKK03_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNX83324.1"
                     /translation="MSLFTRTNRELSVRIDEFFDTIEIGILIFKEGIKAYVNGDTNAF
                     RNHLSKIDDLEGKADKLQRSIENDMIVHSILPQQRSEIVKLLWQLDEIIDTAKKSLNE
                     FYVEMPRIPASLSHEFISLSEVSGNSAEELMPAARAFFREPHLVREKLIKVYFFERET
                     DKAAFQMKKKVFHEMNDLSLAEKAHIRYITHHIENISDTAQDAADLLASMAIRQML"
     gene            10490..>11532
                     /locus_tag="PKK03_02665"
     CDS             10490..>11532
                     /locus_tag="PKK03_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNX83325.1"
                     /translation="MIFLFLTSGLFLGWTLGANDASNVFGSAVGSRMVSFTRAAVTAA
                     VFVIIGAVVQGGGAADTLSRLGSVNAMGGAFTVALAAGLTVYFMTRAGLPVSTTQAIV
                     GAIIGWNFFTDNAVDTRALTQIVSTWITGPVLGAIFAYALYHLVKTARRRIRMHLLKY
                     ENWLRMGLMTVGAFGAYSLGANNIANVMGVFVPSIPLEDADFGIFVLSGAQQLFFLGG
                     IAIAAGILTYSKKVMQTVGTNLMELSSEAAFVVVLAQALVLFIFSSSSLSGLMVRIGL
                     PPIPLVPVSSTQVVVGSILGIGLYKGVRNINLKVLGSIASGWITTPVAAGILAFLMLF
                     IVKNLFSIDVGHSV"
     assembly_gap    11533..11632
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11633..11917
                     /locus_tag="PKK03_02670"
     CDS             <11633..11917
                     /locus_tag="PKK03_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HNX83326.1"
                     /translation="SVVCLKVHGMYFASPAKPGEVLKFESKIVYTGRSSLVAHISVCK
                     STCPKTLVEGFVTFIHVDEETRPLPHNIVTEPLTEADRLLSDQAADLKRS"
     gene            complement(11957..14008)
                     /locus_tag="PKK03_02675"
     CDS             complement(11957..14008)
                     /locus_tag="PKK03_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNX83327.1"
                     /translation="MKKFLLIGTIAFMGTALLTSCGQAGKKAAPEAEFNYEVDRFEDI
                     SVLKYRLPAFEQLTSGEKEFIYYLSEAALCGRDILWDQNFKYNMLIRKTIEAIIDTYD
                     GDRESTDYKAFITYAKRVFFANGIHHHYSNDKFVPGFSADYFAGLVSGSDAARLPLKE
                     GQTAGDLTAMLTPVLFDPALYARRVDQSEGADMITASANNFYEGVTQAEVEKYYASLA
                     DPADPRPVSWGLNTKVVKADGKVTEIPWKSGGMYGQAIDKIVYWLEKARDVALSETQK
                     NELDLLVQYYRSADLKTWDEYNVVWAGNTGLSVDYINGFIETYGDPLGMKATWEAVVD
                     YKDTEASKRTALITENAQWFEDHSPVDGKYRKEKVTGVAASVINVAMLGGDCYPASPL
                     GINLPNSNWIRTEAGSKSVTLANITDAKDIVSRGSGFLEEFACDQAEIDRAVKWGSLS
                     DALHTDMHECIGHASGKLAPGTDPNALKNYASPLEEMRADLFALYFMMDPKMTEIGLM
                     PTDEPARAQYDGYIRNGFLTQIVRIQPGKDIEQAHMRCRSSIAHWVYEKGRSENVIEV
                     VKRDNKTFVRINDYQKLRGLFGEMLKEVQRIKSEGDFAAGRDMIENYGVKIDQELHGE
                     ILARYAGLNLAPYSGFVNPVMTPVTDSNGKITDVKIEYCSDFLGQMMEYGKKYSFLPV
                     W"
     gene            complement(14078..14836)
                     /locus_tag="PKK03_02680"
     CDS             complement(14078..14836)
                     /locus_tag="PKK03_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HNX83328.1"
                     /translation="MLKEIKILADHGKQYVPDLRNQPVSALFRGRPVISADITAALAG
                     RAAALCPAGAIDGNSGAIDLGRCSFCNECALAMPEVYRFTNDYRIAATRKEDLVIKPG
                     QADPVRIDETAVRKEIRRLFRQSLKLRQVSAGGDNSCEMELGATGNVNFDMGRYGIEF
                     VASPRHADGIVITGPVSENMSRALEICYEATPAPRLIILVGTDAISGGLFAGSPAIKR
                     NFIENHHIDLYIPGNPAHPLTFINGLMDLLGKKQ"
     gene            complement(14854..16374)
                     /locus_tag="PKK03_02685"
     CDS             complement(14854..16374)
                     /locus_tag="PKK03_02685"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNX83329.1"
                     /translation="MSSNRYTVVRNGSSPAGFSEIPVIGYDLFHEEVTSMLTDERYHC
                     LSYSCFRTGEGFRFICIIADDGSGEIRILSHIKKDDGTSLKSLTASCYAMHIFEREIH
                     ENHGVVFEGHPWLKPVRYPHDRADRLSVMDNYPFYSMESEELHEVGVGPIHAGVIEPG
                     HFRFICNGERVLHLEIQLGYQHRGIEKLLTSAGDSLRRALLAEDIAGDSAVAHGLAHA
                     QLAESLAGFEPPVSLQAERCIALELERIAVHIGDTAALCGDVAYQLGQVACEALRTMV
                     INTTQFWCGNRFGKGLIRPGGSNFPLTEESAVEILRVLEDVDRRYGNVTGCVYTLPSV
                     LGRFEDIGNVTRKQATSIGAVGMAARTCGVPRDMRATHPFQYYRKMEINPVMQETGDV
                     LARGMLRALEVKESVEIIRRLIDSWRSGAKETGRPQYDLKFRPDCLAVSATEGWRGEI
                     CHVAVTDSDGKLAMYKVKDPSMHNWMALVLAVRSQEISDFPVCNKSFNLSYCGNDL"
     gene            complement(16380..17807)
                     /locus_tag="PKK03_02690"
     CDS             complement(16380..17807)
                     /locus_tag="PKK03_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNX83330.1"
                     /translation="MMLLLFFILSLALSGVTLLSRNRGLTRAFALAFSALLIAITVHA
                     WINRGNTELTWFTYDSTAVLLLSVLTLLTLPTVYHGFIYTRDDTVKRLNIYHSGLIAL
                     MTFMAGAYLANTMTVLWIFVEATTLAVAVLIYHDRTEMALEATWKYVFICSTGIALAY
                     LGILFLGFIYGRGDAPNLSFSSFSHVISNANPLYLKIAFIFVLVGFSTKMGLFPMHTV
                     TIDAHSVAPPPVSALISTTLMNVGFLAIYRVYTLFSGTPALSFMSNVLILAGVLSLLI
                     AAGYMLKAKHLKRMLAYSSLENMGLVAIALGVGGYGYYAALLLIVLHSLAKSSLFYQM
                     GQLSRILHTFRLDESGRYMKLYPAGAMVLMTGMICILAIPPSGMFISELMILRAMVVN
                     GQWLVMALTVILLCYIIYAMSTRIMHVSFSSPRNDSELLQPGMVSPVETVSQFILLAL
                     VIMICFWQPPFLTDLISTSISSLPR"
     gene            complement(17810..18424)
                     /locus_tag="PKK03_02695"
     CDS             complement(17810..18424)
                     /locus_tag="PKK03_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83331.1"
                     /translation="MVNYLIVLFAVTLIYFASAERLSTYVRLVALQGLLLCGIAVFEL
                     KEVNLANLLFIVAETLLFKTLLLPYLLSRIIRKSGVTKVHRQAVSGFYLLLFSIAGLL
                     ISVILAVILVNPFINSIYLAIALFTLLTGLLLIVTHKLIISHLIGFLVVENAVFLFSL
                     AIGNEMPMLINIGILLDIFVGVLILGFFGLRLKPHTNELTMLKD"
     gene            complement(18431..19342)
                     /locus_tag="PKK03_02700"
     CDS             complement(18431..19342)
                     /locus_tag="PKK03_02700"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNX83332.1"
                     /translation="MAGFVLIAVAAFFFPGVILRTKSIASGRKGPGIFQPWKDIAVLF
                     RKGSVFSDTTGLIFRIAPSVTLATVTGAMLFLPFAEQKAVLSFEGDFIMFAYLLALGK
                     FFTIIAALDTGSSFEGMGASREALFSMLVEPAFFILIATFAMFTGYTSFSVIFNHFFI
                     TGNDYVLIYSIIGAYLLVQIAMIENSRLPVDDPKTHLELTMIHEVMILDYSGFDKALI
                     HIGTYLKFAIYGSLIYNLVVPAGWNVILQTGLFFAVQALFAVVIGFTESFRARNKMNK
                     NPKFILTLSSIALIAFMVILILTEKLA"
     gene            complement(19345..21339)
                     /locus_tag="PKK03_02705"
     CDS             complement(19345..21339)
                     /locus_tag="PKK03_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNX83333.1"
                     /translation="MFGSTAFLVILLLSLFVFAVPEWLKYYFTLGLLTAGIALSTSWC
                     TGVLPDAGQTCELSLFTAATGTRMVLVIDSLSAFFILVINFTVLVGFLYARGYLEPYR
                     ARMNALRFSIHYFSYLWLWISMLMVVMVRDGLSFLIVWEIMALSSFFLVIFDAEERSI
                     MKTGISYLIQMHVGMFFILVAFLIVQQDTGRMSFDALGEYFGRHRNLPVFLLFFAGFA
                     IKAGFIPLHTWLPEAHPAAPSHVSGVMSGVMIKMGIYGIVRVLAAMQSDLLVTGVIIL
                     IISLVSGVLGVMMAIVQHDLKRLLAYHSIENIGIIGIGLGLGVIGQATGNHTLSLLGY
                     SGGLLHVLNHSLFKSLLFFNAGSVYLAAHTRNMEQTGGLMKRMPWTAILFLTGSLAIC
                     GLPPFNGFISEYLIYMGMFGSLSGANLFHSILIVGSIAALSLIGGLAIFCFTKAFGIV
                     FLGEPRSDQAGHAREAGKSMIYPQLATAAMIIFIGLGSPLVVKPVFGIVGQIWNLGAQ
                     PLVSGAFTANLAQLSLAGGIFVVTVAVLLLYRRYHLSRRVVETGPTWGCGYTAGSAKQ
                     QYTATSFAYNYNHIAKPLLQTRKEIEEIGEDEIFPHGRKFLSHSDDLFKRHLVDRPVD
                     LVSGLLKRIAVMQTGQIRHYILYAFVFMLLVLLLAMLKII"
     gene            complement(21420..22883)
                     /locus_tag="PKK03_02710"
     CDS             complement(21420..22883)
                     /locus_tag="PKK03_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX83334.1"
                     /translation="MKTNRRNFLRTAAVAGAGTLTAVSTSSCVRREKSTEAMAEVRAA
                     AMAVHNQSFNMSGYAAPPLGKIRIGFIGLGMRGPGAVERMAHIEGVEIASLCDKYPDR
                     VEKAQAMLTKFGLPQAKGFSGSEEAWKGLCDDPDIDLVYICTPWAWHTPMAVYAMEAG
                     KHVAVEVPAAKTVEECWQLVEVSERTKKHCMQLENCCYDFFELLTLNMARQGLFGDIV
                     HGEGAYIHDLRWDFTYDKNGYADMWRLKENVRNGDLYPTHGLGPVCQIMNINRGDRME
                     YLTSLSSNDFAMGPELEQLAKEDPFYAPFAGKSYRGNMNTTLVKTALGRSIMIQHDVS
                     SPRPYSRIHLVSGTKGFAQKWPSPARIALGHSWLKPEDQAALEAQYTPEIVKRVGEMA
                     KVIGGHGGMDFIMDWRLIDCLRNGLPLDQDVYDAALWSVITPLSEWSVANRSSSIDVP
                     DFTCGSYKANLPFDPNIPAGGNTMVIGLKGGNNQLEI"
     gene            complement(23023..23256)
                     /locus_tag="PKK03_02715"
     CDS             complement(23023..23256)
                     /locus_tag="PKK03_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83335.1"
                     /translation="MKKDLLRKYLPEIIGTVAGAAGGFLYWKYVGCVSGTCSIKSNWY
                     LMIPWAAVLGYLAGSVAGDLIRKRKRPPEQEGH"
     gene            complement(23253..24389)
                     /locus_tag="PKK03_02720"
     CDS             complement(23253..24389)
                     /locus_tag="PKK03_02720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX83336.1"
                     /translation="MRAIVAVISDLSTDMRVQKHSAVLSSMGFSVTLIGRHMEGSLPL
                     SLPSAAVAMIRVPFRKGPMMYLWFNVALLFRLLLLRRADLYIANDLDTMIPCHMVSRL
                     FRRPLVYDAHEYFTGQYSLTERKFKRSLWKNAERRLLPKVRYMMTVSDSLAEIYSQEY
                     GCEPVVIRNLPMPVLNILPYQRRNLAASADDLLVVFQDGSGDNPGRGVSELLEAMTLL
                     ENVKLVIIGTGKRIEEARQQVQNQRIDHKVIFLPRMRWEEMMGFIKCCDAGLSIDADT
                     CANQRFSLPNKLFDTIGAGVPVIVSPLPEVSAIIAKYRCGLVLDELTPRAIAGALSLL
                     RDDQQLLSDLKGNALSAAEELSWEKEQIAERDLFIRVIKENSIK"
     gene            24492..25640
                     /locus_tag="PKK03_02725"
     CDS             24492..25640
                     /locus_tag="PKK03_02725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX83337.1"
                     /translation="MERAPAKRIVHLVSFNIPYPPDYGGIMDVFYKISALKRQGVGVI
                     LHCFSYGRGRSRTLERECLRVHYYRRELNLFHLFRKEPFIVVSRRNDMLLKDLLTDSH
                     PIIFEGLHTTHFLGHKALEGRITMVRTHNIEHVYYRNLASNEKNPFRKLYYSTEARKL
                     ERYEPKLAGATWLLSISPGDTEYFSSKYGNAMFVGPFHPADGSFPGNGRGDYVLMHGD
                     FSTAENDAAALYILKEVASHWKYRTVIAGKRPSAELSDAASALRHVKLVPNPSLTQMN
                     ELIANAQVCLLNATQPTGMKLKLINALTTGRHVVASPAIVSGTGLGELCHVAIKPGEW
                     ISLTDSLMKEDFTGAMRARRNLLLKEVADNDNNAKKIVETIDSYNPEL"
     gene            complement(25649..26674)
                     /gene="mltG"
                     /locus_tag="PKK03_02730"
     CDS             complement(25649..26674)
                     /gene="mltG"
                     /locus_tag="PKK03_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNX83338.1"
                     /translation="MRKILLTLFYTLLAAGFVLVLLLWVIVLRENVKHDEPVRIYLPT
                     GSDFRALTDTLERADLLRSRESFIITSRLKSFGKTVKPGSYVIEPGMSNNSLVNMLRS
                     GRQTPVNVTFNNIRTLEELAGKTGGQIEADSASLAEFFHDESNYSADGFTRGTLIAVF
                     IPDTYELYWSVDPRGFYRRMLKEYRDYWNDDRTAKAESLGLTPPEVSVLASIVDEEAS
                     REDEKSRIAGVYLNRLRLGMPLQADPTVKFAVNDFTLRRILNRHLEVDSPYNTYKYAG
                     LPPGPIRCPSRSGLDAVLNAEKHEYLFFVARYDGSGYHHFSRTLAEHNRYAAAYRREL
                     NRRRIYQ"
     gene            complement(26686..28167)
                     /gene="cls"
                     /locus_tag="PKK03_02735"
     CDS             complement(26686..28167)
                     /gene="cls"
                     /locus_tag="PKK03_02735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HNX83339.1"
                     /translation="MNGSRMILAGNTIYIIRNVLTVIYLIAVFAICLMIVFENRNPLK
                     TLSWLLVILFVPVVGIIVYFFFGKNYRKQKMYTRKSMADSARLTDHADRQVRLLSSVL
                     AGESDAIRSKSHLIHLMLRNNRSLLTLDNQIDLLVNGSQTFPAMLDAVASATGFIHLE
                     FYRFEPDTIGMEFSELMMRKAREGVKVRVIYDDVGSWNIRKPFINMMRDAGVQIFPFL
                     PVRFPSFSSKINYRNHRKILVVDGKVGFVGGLNIADKYLHGLPHLGPWVDTHLRVEGE
                     AVAALDRVFLADWDFVSGEELPPDGSLEHVVRTGNRCLVQVASSGPDTDWATIMQVYF
                     SAIATAKSSIYLTSPYFSPDESLLTALKTAALSGVDVRMIFPENSDSIIANWNTRSYI
                     MELLEAGVRIFLYRNGFIHSKYLLVDSIFSSVGSPNVDVRSFDLDFEVTALIYDEDFA
                     LRLGVLFAEDLKNCAEVTIQEWSKRNRRERYRESLARIFGPLY"
     gene            complement(28239..28856)
                     /locus_tag="PKK03_02740"
     CDS             complement(28239..28856)
                     /locus_tag="PKK03_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX83340.1"
                     /translation="MKKFIVIILALVLAAPLFSQVKFGLKAGASTDFTFTDQTFKGTD
                     FEVILQNAKDAEWGFQGGVFMRATFSGFYIQPELLLATATNSVSYEDVEGGGAPVIYN
                     QKFNKLNIPVLLGVKVGPLRLNAGPAASVMISDPKEIIQGATYKRATFGYQAGLGFDL
                     FKRLTFDLRYEGNLNQFGEEIEIGDQNFQLDDRTGALLLQVGLIF"
     gene            complement(28958..29485)
                     /locus_tag="PKK03_02745"
     CDS             complement(28958..29485)
                     /locus_tag="PKK03_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNX83341.1"
                     /translation="MKRTVLMVIALLVVAALSHGQKVYENNYLVKVGDSAPDFAINEA
                     GGKSYKLSDLKGKVVMLQFTASWCSVCRKEMPFIEEEIWMPGRKQGLVVIGIDRDEPE
                     ATVRKFAADMKITYPLALDPGADIFGLYAEKQAGVTRNIIIDRNGKIIFLTRLFEREE
                     FDKMKSVIFSELAKK"
     gene            complement(29591..30922)
                     /locus_tag="PKK03_02750"
     CDS             complement(29591..30922)
                     /locus_tag="PKK03_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83342.1"
                     /translation="MVVFLLSSALSSGQDNPEYRLRLDIPLIDLPQNIDLPYRYPSMN
                     QALEFSSGMYDLAYWGIDELGDAIFTSRSKPETRFGRFTNSAFKYALGLGFSKYGSEL
                     PIPLGVWAHEEYHRAVLGTSGVSSKNGNWFLSRWDGTVYGISDMTLQDLKTTGINNLL
                     YSYVAGVQYEINLNERIAISEFYFERSLPKASLLLYNAWYVYNYFRFSASPASDSVKV
                     LAPPHENTNPAERDYAGADLNAWIHDMFNPGLPYTERDPFPGGEGVNRRIGFSDLSSE
                     GQDYLTRQKDLSLLNFLNPGIFFIDRIKFSEGFSFNFFTQYSPTHFGNDIALFVPVRF
                     RRFGVMVNAHRYSNKSDTGLGAGLSLYNLGVSGKVRSELRLNVWSQPESFFSDSMVTG
                     GYAGIRTRYLFNDSFSGFVSVSGKTKGWLLGNPYLDKNVSFQAGLSYELLK"
     gene            complement(31117..31536)
                     /locus_tag="PKK03_02755"
     CDS             complement(31117..31536)
                     /locus_tag="PKK03_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83343.1"
                     /translation="MELFYLYENILAVERVCLDCGARLHGRSDKKFCSDQCRNNYNNR
                     LNRDQNNYVRNVHAQLRRNRKILSDLYGDGHHRIHRDALIAQGYNFTFFTHLVETREG
                     LRWSYCFEFGFRESDEGYLELIKSSVYLEAIIDEEEV"
     gene            31707..32171
                     /gene="ssb"
                     /locus_tag="PKK03_02760"
     CDS             31707..32171
                     /gene="ssb"
                     /locus_tag="PKK03_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNX83344.1"
                     /translation="MNALRNKVQLIGRLGQDPEIKTLESGKKVARFNLATNESYKNSE
                     GAKIDETTWHSIVVWNGLAELTSKYLTKGKEVCIEGRISYRSYTDKNGIQRNVTEIVA
                     SDMVMLGSGARPRGLIGDEPGEDMADDPAAGLADEPVEAGASTHRGSRVRMK"
     gene            complement(32361..32939)
                     /locus_tag="PKK03_02765"
     CDS             complement(32361..32939)
                     /locus_tag="PKK03_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HNX83345.1"
                     /translation="MKCDIILAGVGGQGILSIAATIGLAAVEQNLFLKQAEVHGMSQR
                     GGDVQSHLRLSDSEIFSDLIPHGGADLIISVEPMEALRYLPWLSESGWLVTNSVPFVN
                     ISDYPEVGEVLAELNRAGNTIVLDADGIARDLGSARSGNMVILGAASSFISMPFTALE
                     DAVRKMFGRKGEEVVEVNLKALRAGRDAAAAR"
     gene            complement(32941..34560)
                     /locus_tag="PKK03_02770"
     CDS             complement(32941..34560)
                     /locus_tag="PKK03_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNX83346.1"
                     /translation="MHKLLLLGDEAIAQAALDAGLSGMYAYPGTPSTEIMEYIQASPQ
                     AAADGVHREWSSNEKTAMEAALGMSYAGKRAMVAMKHVGLNVAADAFINSAVTGVNGG
                     LVVVSADDPSMHSSQNEQDSRFYGKFALVPVMEPRNQQEAYDMIFEAFALSEKYRLPV
                     LFRITTRLAHSRSSVARREKIAQNSVALPSNPRQFVLLPAIARKQYRALTAVYEKLRT
                     DDAVDGLNRLKSGNGRGTGVITCGIAYNYLMENIADRAGDYHVLSVGAYPLKREKVAA
                     LVENCDRVLVLEEGYPIIEEQLRGLLDRDVKVLGRMDGTVPRDGELNPSIVASALGLR
                     AGQGREVPSLVRQRPPSFCKGCGHADLYQALSEVMAEAGPGRVFSDIGCYTLGALPPY
                     NMVNSCVDMGASVTMAIGAADAGLFPAVAVIGDSTFTHSGMTGLLDAVIKNSPVTLII
                     SDNSTTGMTGGQKSQATDRLESICLGLGVDPDHLKVIVPLRKNHEENKAVIRAELNYH
                     GISVILARRECIQTASRNKKAEAAEKNKSRE"
     gene            34678..35358
                     /locus_tag="PKK03_02775"
     CDS             34678..35358
                     /locus_tag="PKK03_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83347.1"
                     /translation="MKKFLVICIMLLFSFPWLGAQTGERKYIQIDGMTADERGFIVPD
                     VSIYSKHLRRGAASDHRGIFSIISAPGDTVFFSSIGYKPTLLTIPVTATGDHYITDVI
                     MVTDTIQIGEVLVLPWKTYSEFKRAVLEDKPRDPLMENMEYNLAMVQQQIWSDMRPTP
                     GEAYHYTMQQMSDQLYTRGQSPANNLLNPFAWQKFVQGVKSGMLKNEPSKAVKVKKHK
                     SRKKKKSD"
     gene            35358..37781
                     /locus_tag="PKK03_02780"
     CDS             35358..37781
                     /locus_tag="PKK03_02780"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNX83348.1"
                     /translation="MVRSSAIRLLTVSLFLSIIPAGFLNGQEKVISGIVTDTAGVAVA
                     GATVFIPSVERGTLCGNDGSFRLAFTPGESESLTVIFSCVGYSADTVTLKADGTLDGI
                     RVVLIPATTIIEGVEVRASRPDNVTVIGIPAVAGAVIPSVSGGVESAVATLPGVSAFS
                     ELSSGYSVRGGSYDENLVYLNDVEIYRPYLLRTGQQEGLSRINPDLTASVNFSPGGFS
                     AAYGDRMSSVLDITYREPESREGSVSLSLLTSSVHAGARSNDNRFWFIAGARYRSNAI
                     LLGGLDTRGAYRPHFADIQAIGGFRPDSRTGITLTLWGSLNRYTFLPRSQTTTFGTVE
                     NAYRLYAFFEGGERDRYNSGGGALTLERKHTDRLLTKLIISSSHAGEYESYDIRGAYS
                     LSALDMNVGSENNPDTLLNAGAGSWLSHARNSFRSLIAGVSYRGSLSSGKNGLEWGLA
                     ARQRSYDSFRNEWLRVDSAGYTPGQPGDGLALTSRTVLGNETRSLTSEGWLISRRRFA
                     VAGRACDLNAGIRASYDTYSSEILISPRVSLRSEITRQVSLYLAAGAYHQPPEGRELI
                     REDGESGSYPKAQRSYHLTAGVRYDFTAWERPFRFTAEAWGKLFDRLIPYKSDNVRLI
                     YYGGNIAEGYSAGIDMRVNGEFVPGVESWFSMSLMKSEIQIPELSTGWYPAPFDQRMH
                     FSIFFQDYLPGHPDFRAHINIAFGTGIPTSPPGHEQWDIWFRMPPYRRIDIGFSKVLL
                     GAGRANRDSFMKELVAGVEIFNLADIRNTISYTWIRTVRNSQGQTREYAVPNYLTRRS
                     LNLKLTAQF"
     gene            37827..>38124
                     /locus_tag="PKK03_02785"
     CDS             37827..>38124
                     /locus_tag="PKK03_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83349.1"
                     /translation="MNRFEITFTAVMATIIIHLTVAVIFMSVKISALRREIAAEIILS
                     IDEEMPDPAMEKAIELAKSDQFQGISPEEMVNIIKNIADKPLDIDPKEYEDMVKE"
BASE COUNT         9564 a         9960 c         9343 g         9157 t
ORIGIN      
        1 agagatggta ctcgagaacg gtctcaccat tctggatgtg ccatactgat cccttgtaaa
       61 ccttaatctc ggcggtgttc cactcaccgg ctggttttgc attctgtggt ttggcgggaa
      121 tcaggtcata tagtgacccg gcctggcggt ttccgtcttt tccggcctga gcatcggggt
      181 gcctttcatt gtcaaggacc tgcatctcgg gagcggtctt gtatatgtaa tcccactccg
      241 gaccttcctg tgccaggtag aatattccgc tgttgccacc ttccgagatt ttccattcca
      301 tcttgaggtg gaaatttgag aacttcttgt catagatgat atcaccgccg ccaccgccgg
      361 cttcacccct cccggaaccg atgcaatgca gtgtaccgtc aacaatctcc catccctgtg
      421 tggggaactc gggcttgttg tagttacgcc agccctgggt gcctgatccg tcaaagagat
      481 aggtccagac ctcggcttca gtcgatttct tttcagcatt ctttttctcc tttcctgatc
      541 cgcaggagac aaacgagacc gcaaggagaa gaacaaacat tgtctttatc agtttcataa
      601 gttatttatt ttaagtatta gttttggttt tagcgttatg cgggcatttc gggaagtgac
      661 cagcctgatc ggtaagtatg cctgatcatt tcggcagcaa actgcctggc attgatcgga
      721 tcagtgaact tcctgtcgaa tgtcggatga ccgtcagtga tgctgaaatg atcctcgaca
      781 catatcttaa tagtttcttc gtcactgata ttggcaaact ccatcttttc tccgttccac
      841 tcgagtatct tgttcagtcc ctggagcctt acggccagga cgcccataac caccatttca
      901 ttgaacggac ctgcttcgga gaaatcagac ttggtaagga ttctgttatc agggttttcc
      961 ttgcatgctc ttacccaatc catctcatga cctccacttg tggcattctt aacacgcctt
     1021 tcagtttcag gagcatttgg caccctgcct gagaggagcc atggattgat gccatagcaa
     1081 ccgcatatga gcttatcctt ggtcccatgg aagatgactc cgcctccctg gtcattcata
     1141 tcctttccga ttggccatcc ttcgggcttc atgggttgaa ttcccccgtc ataccatgtc
     1201 acctccactt caggcaggtc aatcttgtaa tgcgatggcg cctttctggc agggtaggta
     1261 tacttgacag tctgtgcgtt tggcgcacag tcggtcagca gaagggttga gcttgcctgt
     1321 acttttgtcg ggtatgtgag cttgagagac ttgaaaacag gatgaaggat atggcatgcc
     1381 atatcaccca gcgcaccggt cccgaaatcc caccatcctc tccagttcca cggatgatat
     1441 attgcgttgt agggtctgac gggagcaggt cccaggaaca ggtcccagtc aagcgtcttc
     1501 ggcaccttat ctacttttga tggccggttc agaccctggg gccatatcgg acggtcagtg
     1561 aaggcttcta cctttgtcac ctcacctatt tcacggttct gtatccactc ctgaacaagg
     1621 tccacaccga tggctgacga accctgattt cccatcgagg ttgccacttt atgcttcgct
     1681 gcaagcttcg tcagaagtct tgattcatat acactgtgcg taaggggttt ctgaaggaag
     1741 acatgcttgc ccatggtcat agcgtgagct gcagtaaggg catgagtgtg gtcagccgtt
     1801 gcaatgatca ctgcatcaat tgacttgccc atttcatcat acatcttccg gtaatcccag
     1861 aaccgcttcg ctgccgggta ggcatcaaag accctcttcg agtagcccca gtccacatca
     1921 cagagcgcca ctatgttttc tgatgcggca acattcctga ggtttgtagc acccatacct
     1981 ccgataccaa ttccggcgat gttgagctta tcgctcggaa cacggtggcc gagcccgctg
     2041 accacgttgc tgggcaggat ggtaagtccg gcaattgcgg cgcccgtagt gcccacaaat
     2101 gaccgtcttg tgaattttct tgtctcctct ttcatatcat ttcaggttaa gttcttcaac
     2161 ggatggataa aaatagaaat ttctccagaa taattctccc tgcgggacta tttcttatat
     2221 ttttgaagaa tgagactcag aagaggcctg acaaccttaa ttatagccaa cttcgggatt
     2281 ttatcctcct atgggcagta tttcaccagg ctgggaatcg gcctcgatac ccggacaaac
     2341 gtaagcatac ctgtggctgt ggctgatccg aatgatcctc aattttcagg agagttactg
     2401 gccgccaagt acccgggcct gaatatcccg cccggaaaaa ttgacctcgt gtacccggtg
     2461 atcccggacg ctgccgatac ggtggccatt ctatggtacc tgatgcccga ggagatctct
     2521 gccgtgcccg gagaaatgaa cgtgatcata gttgaaatca cccccggaaa tgaaaaaaga
     2581 ttctggattg acaataacaa tgatcgcgtg ttcagcagca gcgagagatc atttattttc
     2641 aggaacactg aagaaagccg gcctgtgaac ataatggtcg caggctccta ttactcctat
     2701 actctgttta accccgacta ctcccctcct tctgcccctt cggaaaccat caggcaatcc
     2761 aaaagctcct ggtcagacag aaagggaaaa ccatcttttg cgattgacct gtcagcgtca
     2821 ttcctcaggg cagatgcaag tctctcatat ataaagcaat caagaccatc aaatacaatc
     2881 acctattccg ccagaatccc cggaagcttc aggcctgcca tcggattcga cttttcatgg
     2941 tatgactttc atcttcttct gggtggcggt ttcgagaaag ccaagtacac tgaaaacata
     3001 ctggtttcaa caaatgacgg cagccagact acaagctaca acagcggaac ctggccaact
     3061 tcgaggttca tcctcagcat tgcactggag tatgacatca gcgccggcag gtatctttac
     3121 ctcacaccct tcgccgtatg gtccaatttc aaaaatgaca gcaggttcaa ctttgacagg
     3181 taccagggat ctagtgcagg tgcagaatac acagactcaa aatcttatga agcaggctta
     3241 aagcttaaaa tgccggttga caggcaggtc atcattcacc tcagcctcgc atactcttcg
     3301 gtgtatttca atgctgaaaa gcattttact gatgttttac ccggatcata ccgcatgagt
     3361 atggaaggat tctattacgg tgtcggcgtt aatttcagcc tcagaggttc ggcagggcag
     3421 gatgctgaat gagccttgta atcaaaaaat attactttgc agaaaaaaca gatgaaaagc
     3481 cagagattta atctcgatcc cggagaaaat gaccgtatta acaggattct gaaggctctg
     3541 ttcggactgg tgtgcattgc tgcggcaatc acaaccggaa taatgatgac cggttcggat
     3601 aagctgacaa ccaacagttt cattgccata gcttttatgg gtcttattgg tataagcctc
     3661 ctgatatcag gactgggcct gaccgaaaga tttctgaccc tcaccgggga cactattatt
     3721 ctcagggaca aggtttatgc ccctgccaga acaataagcg tgtctgatct gagaaaagtt
     3781 gaattcagcc agcttaaagt tgcattttac ctgaagtcag aagaggttat atcattgcgt
     3841 ttggggacgt tttacaggga gagtcccgta aggattatgg aggctcttga gaaattctgt
     3901 gcctcaaacg gcgttttgac tgaaggattg ccatctaaca gggacgacga cggtcatgaa
     3961 aattagatat ggaatgatcg gcggaggcga aggctcgctc atcgggcccg tacaccggat
     4021 ggcagcacgc ctcgacggta tctgtgagct tgtttccggt tcattcagca gtaacccgga
     4081 aatctcgcgg ctgacaggaa ccagggaggc tcttaatgaa gcacgcatct atgacagatg
     4141 gcaggagatg atcacaaccg aaagcaccct gcccgaagag gtcagacccc acttcatcac
     4201 catagtcacc ccgaaccatc ttcattacag tccggcaaaa atggccctgg aggcaggatt
     4261 tcatgttatt tgcgacaaac ccctatgtct cagtgtatct gaagctctgg acctccatga
     4321 aacagttgag cggacaggga aactgttttg cctgacccat aattataccg gttatcccat
     4381 ggttaaggca gcacgcaata tggtgcttgc gggggagatc gggaaagtca ggaaggtgat
     4441 tgttcagtac ctgcagggat ggctcgcgtc accgctggaa agaattggca accggcaggc
     4501 tgaatggagg tctgacccgg caagggcagg catagccggg gcaatggctg acataggaac
     4561 acatgcattc aacctcgcgg aatatgtaac gggcgaagag gttgtctcat tgtcggcaaa
     4621 actgaattca gcggttcccg gaagaatcct tgacgatgat ggcacagtct ctctcacctt
     4681 tggaaacaac ataagcggga ctcttcttgc cagccaggcg gctaccggag aggagaacaa
     4741 cctgtcaata aggatttacg gtgaaagcgg agcacttatc tggcgccaga tggagccaaa
     4801 taccctcacc cggctccgtc ctgatgcccc tgcgcaggtt atccggaccg gcgcctcctt
     4861 tgccggatcg ggcttaccgg cctctatgca cacaagactg ccggcagggc atcctgaagg
     4921 attcattgag gcattcggca acctgtacag gaattttgca ttgcatataa tagcagtcaa
     4981 cgatggtcag aaacctgacc cggtggctga ttacccgggc atcaatgagg gagtcagggg
     5041 aatgaaattc ctggaggcgg tggtctcttc ttcacggaac ggatcagtca cgataaaact
     5101 ctgagaagaa tatagcaagc cccccgggca cgagatgccg gagggctgca gtacatcttt
     5161 gccggaaatg atttccggag tcttattttt tcagaagtcc gaggtaataa tccctgctta
     5221 tcctgatgct ttccatcgga gtatgcgtgc ggcaaaagtc ctgctccagg aaccagtatt
     5281 tcatgcctgc tgtccgggcc tcagcaagaa tcggcctgaa atcgatgatt ccctctccaa
     5341 gggttgcatc ctgtttctcc ggggtaaggt ccttcagatg ccatacctcg aaccggccgg
     5401 ggtatttcct gaagtaggcc accgggtcct tccctgcagc ggatatccat gccaggtcca
     5461 tttcaaagat taccagcttt ggatcactct gttcaacgag tatatcataa ggcacacggc
     5521 catttatctc cctgaactcc acctggtggt tgtggaaccc aaaggtgatg ccggccttcc
     5581 ggcacttcgc ccctgccctg ttaaagaaat cggctgcccg ctggtaaccg tcaatagtgt
     5641 tgctccagtc accgggaagg gatggcagta ttatatactt catcccggcc tcagcagctt
     5701 cgtcaatcat cttctcgtaa ttttcaggat tcagaccgct gtgtgagctg atggcaacca
     5761 ttccgttctt ccttaccagc ttactgaatt ccttcggctt catgccataa aactttccgt
     5821 cacggtggtc agctgcctca atccatgtgt aacccgtttc cgcagccccg gcaagcgcag
     5881 cggcagggtc cttgaccatg gcgtcacgta tggtgtacaa tgcaagacct gtcttctttg
     5941 aaggcctgga tgatgcaatc atctcgctgt tgatcatcgg tgctgcagca atgacaaggg
     6001 ctgatttcct taaaaagtct cttctggtag tcataatttc aggttaagtt ttaacggatc
     6061 atgaagttgc caccccaatc taaccaggga tgaagggaaa acaaaaatag cattaaattt
     6121 ataagatcaa aagccaaatg cagatgaggt tgtgggagaa acataaggaa ggcagcgtga
     6181 gatgtaccct gtgccctcat tactgcatca tcaggcccgg agagagaggt atctgcggaa
     6241 ccagggaaaa ccgcgaagga gaaatttacc cggttaccgc cggcatcatc tccggatatg
     6301 ccctcgatcc cattgaaaag aaacctctct accacttcca cccggggtcg gtgattctgt
     6361 cagtaggatc atacggatgc aacctcaaat gcgatttttg ccagaactat cacatttcac
     6421 aaaatgtcaa aggggaacag tcagcaaggc ttgatccgga agagcttgtc cgtcaggctg
     6481 taacagcccg gggaagcatt ggcatcgcat atacctacaa tgaaccggcg atatggtttg
     6541 aatacgtaac tgaaagcgcc aggcttgccc gtgaaaaagg gctccgcaac gtaatggtta
     6601 ccaatggcta tgtaaatcag gaaccgttga aggaactgac acaggtcata gacgcattca
     6661 acatagacct gaaggcgttc agcaacgact tttaccggaa ttataccgga gccaccctta
     6721 agccggtgct gcaggcaata aagactgtgg ccggatcagg caggcatctc gagataacca
     6781 ccctggtcct tccgggaatg aacgattcgc ctgatgagat ggaacgggag gctgagtgga
     6841 tctccggaaa tgcgggcagg tccgttccgc ttcatctcag ccgctacttc ccgacataca
     6901 ggagaaccac acctgcaacg ccgcccgaaa caatactaag actcagggaa atagcagaga
     6961 agtatctcga ttttgtgtac accggcaaca tgcccggtga agagggtggc agtgacacga
     7021 ggtgccccga ctgccatacc acagtgataa gaagatcagg atattatgcc cggatttccg
     7081 gtcttacgga tgaaggaagc tgcgtaaaat gcagccggca gatcatagcc gcatcggatg
     7141 tctgatatta ccggaacgaa tatctgatgt gtggccggaa ctactgttta gtaatactgc
     7201 tcagcttctc gaagaattca ttgatcttgc cgaacagctc cttgcggagg tccgcataat
     7261 aagccctgac aagtgccgga ttatcttttc catcggggtg atttgccctg tcaatgaatt
     7321 cattgcgcat aactaccgct tcttcaatca ggttctgcac ctccatcttc ttggctccgt
     7381 catgaagact tgaagccata aaacaatcag agatcagctc gaagaagaga aaatcgatat
     7441 cctttttcag acttttaata ctggccattg ctgttggttt tgatgcaaat atattgatta
     7501 atccgcttca tcccccattt tgtaatcatt catgctccgt ccggcaaggt ctgagtaagc
     7561 ttcgcgaagc cttccaatga gcggatcggc gacattaaac tgcagaccct caatgctcct
     7621 gacggcaagt accataggag aggtgccggt aatgaaagcc gacctgaatc tgccggcgtc
     7681 actctctttt acggcttcag taaccagggt gatcccttcc ttcaggataa tatcagtcac
     7741 atatctcctg gtgatcccgg aaaggaccat gctgtcaggg gccgtttgta ttgtcccgtc
     7801 ttccgcaatg aaaaagatgt ttgacctgct cccctctgta atgaatccat cgtgatttac
     7861 cagcagcgct tcgtaagcgt tccttcttgc cagctccccg ttgaccgaga gacgtagcct
     7921 gttgttcagc atcttaaccc ccggatcaag ccgttcggca tggtgaagaa tcagcggcac
     7981 tccctctctt accatcgttt ccgacggata gaatgaagga atatagcaga tatgcagaga
     8041 gtggtcatac ccgctgaagg acactgtcac cctgacattt atttcatcat gccgctcgtg
     8101 gtttgcaagc gctttcattc ctgccctgac ctctgcagcg atatcctccc gaaggtcata
     8161 ccttgtggca ataccatcct taaggcggtt catgtgatca ctgaagaaga gaggtactcc
     8221 gtcacgggtt ctgaccacct catagaaggt gacctcgccg gaagggatct cctcattgcc
     8281 cgatgcgggc actatggctc cgtcaagcca gatgtaactg cctgtgcaat ccatgtccgg
     8341 atttctattt ttatcaaaaa taggaataat gtcgcagcct taaaagtaat tatcttagtg
     8401 ctttcccgaa gagatgtatg ccatcgtaga catagagaca acaggtggaa gcgcgaggct
     8461 tgagagaata accgagatag ccgtttacat acatgacggc aaccgggtgg tggaggaata
     8521 ttcgaccctg atcaatcccg agcgcaatat tccctacttc atcacttccc ttacaggtat
     8581 aaccaacgag atggttgagg acgcacctaa attttatgag gtggcacgcc ggattgtaga
     8641 gcttacggaa ggaaaggtat tcgtggcaca caatgcaagg ttcgattatt cattcatcag
     8701 gcaggaattc ggcatgctgg gatacaattt caaacgcccg atgcttgata ccgttgccct
     8761 gagccgcaaa ctgattcccg ggcacaggtc ttacagcctg ggaaaccttt gcaatgacct
     8821 cgggatagag atcaacggcc gccacagggc ttcgggtgat gccctcgcaa ctgtgaggct
     8881 tctggagctg ctgctcgaga aagaccgtga gctgaattcc ggcagcctga tcaaaaacag
     8941 gaaagcggcg aaactgcacc ctgcacttga catgacaaag cttgaagaga ttcctgaaga
     9001 acctggagta tactacttcc atgatgaagg cggagatgta atctatgtgg ggaaaagccg
     9061 taacctgagc gagcgggtca gctcacacat ctcaaacaac acctcggcaa gggagatgga
     9121 gatgaggtca atgattgcag acatcacctg ggagcgcaca ggaagtgagc tgattgccct
     9181 gctgatggag tcagccgaga taaagagccg taaacctcgc ttcaacaggg cacagcgacg
     9241 caccgggttc cgctggggta tctacagtga aacggatgag gccggataca tcaggttctc
     9301 atacagaaat gtcagagatg aggaagtgcc tcttgcggta ttcacctcac gtgacagggc
     9361 gcggggcaag ctggagcaga ttatcactga ctacaacctc tgtcagaaac tctgcggatt
     9421 gtatgaaact tccggaccct gtttccaccg ccaggtgagc atgtgccgcg gggcctgctg
     9481 cggagaggag gatccgaagc agtacaacga aagagccctc atggctctcg atgaattcat
     9541 cttcagggaa agaaacttct ttatcattga ccgcggacgc gatgagcagg aacggtcagc
     9601 tgtcaagata gtcaacggca agtttgcggg ctacggattc ttcagcatca acgacgtggg
     9661 attcgggctg acggccgtac atgattgcat aaaacccgca aatgacaatc gcgacatcca
     9721 ggtcatcatc aagggttacc tgaagaaaaa ccgggtggag aaaattattg atttctgact
     9781 cttcggactg ctgcgctttt cttaatatat ttgccttaaa caacacaagc ccgcatatgt
     9841 cgctttttac ccgcaccaac agggaactca gcgtaagaat tgatgaattc tttgatacaa
     9901 tagagattgg cattctgatc ttcaaggagg gcatcaaggc atatgtcaac ggtgacacaa
     9961 atgccttccg gaaccacctc tcaaagatcg atgaccttga aggcaaggcc gacaagctgc
    10021 agcgcagcat cgagaacgac atgatagttc attcaatcct gccgcagcag aggagtgaga
    10081 tcgttaagct gctctggcag ctcgatgaga ttattgacac ggcgaagaaa tcgcttaatg
    10141 agttttatgt cgagatgcct cgaatacccg cttcactgtc acatgagttc atttctctct
    10201 cggaggtctc gggtaactcg gccgaggagc ttatgccagc cgccagggct ttcttccgcg
    10261 agcctcacct ggtacgggaa aagctcatca aggtctattt cttcgagagg gaaacggata
    10321 aggcggcgtt tcagatgaag aagaaggttt ttcatgagat gaatgacctc tcactggctg
    10381 aaaaggctca tatcaggtac atcacacacc atattgagaa tatttcagac acggctcagg
    10441 atgcagccga cctcctcgcc tccatggcca taaggcaaat gctctgatca tgattttcct
    10501 gtttctcacc agcggtctct tcctgggatg gacactcggc gccaacgatg cttccaacgt
    10561 gttcggatcc gccgtcggtt caaggatggt ctccttcaca cgcgctgcag ttacggccgc
    10621 tgttttcgtt attataggcg ctgttgtaca gggtggtggc gccgctgaca cgctgagccg
    10681 cctcggatca gtaaatgcaa tgggcggtgc ttttacggtg gcactggcag ccggattgac
    10741 tgtatacttc atgacaaggg ccgggttgcc ggtatcaact acacaggcca tcgtgggtgc
    10801 tatcatcggc tggaacttct ttaccgacaa cgcagtcgat accagggcgc tgacacagat
    10861 cgtatctacc tggatcacgg gaccggtgct cggagcaata ttcgcatatg ccttatatca
    10921 ccttgtgaaa acagcaaggc gaagaatcag aatgcacctc ctgaaatacg aaaactggct
    10981 gcgcatgggt ctgatgaccg ttggcgcatt cggcgcttac agccttgggg caaacaatat
    11041 tgccaacgtt atgggtgtat tcgtgccaag catccctctt gaggatgcgg acttcggcat
    11101 ctttgtgctc agcggggcac agcagctctt cttcctcggt ggcatcgcca tagcagccgg
    11161 catactgact tattcaaaga aggtgatgca gacggtagga accaacctca tggagctttc
    11221 atccgaggcc gctttcgtgg tggtgctcgc acaggcactg gtactgttta tcttctcatc
    11281 atcttcactc tcaggattaa tggtaaggat cggtctgcca cccatacccc tggtaccggt
    11341 ttcaagcaca caggtggtgg taggttcaat acttggaata ggtctttaca agggagtcag
    11401 aaacatcaac cttaaagtgc tcggaagcat cgcctcaggt tggataacca cccccgtggc
    11461 tgcaggaata cttgctttcc ttatgctctt catcgtaaaa aacctcttca gcattgacgt
    11521 aggacactcg gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagcgtggt
    11641 ctgcctgaag gtccatggaa tgtatttcgc cagccctgcc aagcccggcg aggtactgaa
    11701 atttgaatcc aaaattgtct atactggcag gtcaagcctg gtggctcata taagcgtctg
    11761 caaatcaacc tgtcctaaaa cgctggttga aggcttcgtt acctttattc atgtcgacga
    11821 ggagacccgc ccactgcccc acaacattgt cacggaacca ctgaccgagg ctgacagatt
    11881 actgtccgac caggcagcgg atctgaagag atcctgattc agactcgctc cccggagcca
    11941 ggctgatcct gacaaatcac cagaccggaa ggaaggaata tttcttcccg tattccatca
    12001 tctggcccag gaaatctgaa cagtactcaa ttttcacatc tgttatctta ccattgctgt
    12061 ctgtgacagg tgtcatgacc ggattaacga atccgctgta tggagccaga ttcagtccgg
    12121 cataccttgc aagtatctca ccatgaagtt cctgatctat ctttacacca tagttctcaa
    12181 tcatgtcgcg gccagctgcg aaatcacctt ccgacttgat tctctgcacc tccttcagca
    12241 tctctccgaa gagtccgcgc agtttctgat aatcatttat ccggacaaac gtcttgttgt
    12301 cacgctttac cacctcaatc acattctcag acctgccctt ctcatatacc cagtgggcta
    12361 ttgaagaccg gcacctcata tgagcctgtt ctatgtcctt gcctggctgg attcttacta
    12421 tctgggtcag gaaaccattg cgtatataac cgtcatactg tgccctggca ggttcgtccg
    12481 taggcataag cccgatctcc gtcatcttcg gatccatcat gaagtaaagg gcaaacaggt
    12541 cagctctcat ctcctccagg ggagaggcat agtttttcag ggcattggga tcagttccgg
    12601 gagccagctt gccgctggcg tggccgatgc actcatgcat gtcagtgtgc agtgcatcgg
    12661 agagtgatcc ccattttacc gccctgtcaa tctcggcctg atcacaggcg aactcctcca
    12721 ggaacccgct tccccgtgac acgatatcct ttgcatcagt gatattggca agggtgactg
    12781 atttggagcc ggcttccgtt ctgatccagt ttgagtttgg caggttgatc cccagcggag
    12841 aggccgggta gcagtcgcct ccgagcattg ccacattgat tacgctggcg gccaccccgg
    12901 tgaccttttc cttgcggtat ttcccatcca caggtgaatg atcctcgaac cactgggcgt
    12961 tctccgttat cagagccgtc ctcttcgagg cttcagtgtc cttataatcg actacagcct
    13021 cccatgtggc tttcataccc agcggatcac catatgtttc aatgaatccg ttgatatagt
    13081 caactgacag tccggtgttg ccggcccata caacattgta ttcatcccat gtcttcagat
    13141 cagcgctcct gtagtattgt acaagcaggt cgagctcatt cttctgtgtt tcgctcagcg
    13201 caacatcacg tgctttttcc agccagtaga ctattttgtc gatggcctga ccgtacattc
    13261 cgccactctt ccatggaatc tcagtcacct tgccgtcagc cttcaccacc tttgtgttca
    13321 ggccccatga aaccggcctg ggatctgccg gatcagcgag cgaggcataa tacttctcca
    13381 cttcggcctg tgtcactccc tcatagaaat tgttggctga tgcggttatc atgtcagcac
    13441 cctccgactg gtcaactctt ctggcataca gtgcagggtc aaacagcacc ggtgtgagca
    13501 tggcggtaag gtcacctgcg gtctgtccct cttttaacgg caggcgggct gcgtcagaac
    13561 cgctgaccag cccggcaaaa taatccgctg aaaatcccgg aacaaatttg tcatttgaat
    13621 agtgatggtg tatgccgttg gcgaagaaga cccgcttggc ataggtgatg aatgccttgt
    13681 aatcagtgct ctcgcggtca ccgtcatatg tatcgattat tgcctcgatg gtcttgcgga
    13741 tgagcatgtt atatttaaag ttctgatccc aaagtatgtc gcggccacac agagctgcct
    13801 cagacaggta atagatgaat tccttctccc ctgacgtcag ttgttcaaac gctggcaggc
    13861 ggtacttaag cacgctgata tcttcaaacc tgtcaacttc atagttgaat tcagcctccg
    13921 gtgctgcctt tttgccggcc tgcccgcatg aggtcaatag cgctgttccc ataaatgcaa
    13981 tcgttccgat tagtaagaat tttttcatga tacggctttg ttgtaatcag gtatctaagg
    14041 tagcaaaaag ctttatctcc tatgggaaat ttactatcta ttgctttttg cccagcaaat
    14101 ccattaagcc gttaatgaac gtcaacggat gggcaggatt gccgggaatg taaagatcaa
    14161 tatgatgatt ttcaatgaaa ttcctcttaa tggcaggact gccggcaaag agccctccgc
    14221 tgatggcgtc agtgccgaca agaatgatta accttggcgc aggagttgct tcgtagcaga
    14281 tctccagtgc cctggacatg ttttcggaaa caggaccggt gatgacaatt ccgtcggcat
    14341 gacggggtga agccacaaat tcaattccgt acctgcccat atcaaaattc acgttccccg
    14401 ttgcccccag ttccatctcg catgagttat cgcctcctgc cgacacctgg cggagtttca
    14461 gggactgcct gaacaacctg cggatctcct tcctgacagc agtctcatct atccggaccg
    14521 gatcagcctg gccgggcttt atgaccaggt cttccttcct ggtggcagca atacggtagt
    14581 cattggtgaa cctgtaaacc tccggcattg ccaatgcaca ctcattgcag aaggagcacc
    14641 tgccaagatc aatggcaccg ctgttgccgt caatggcgcc tgccgggcat aacgcagcag
    14701 ctcttccggc cagggctgca gtaatatctg ctgagattac cggcctgccc ctgaacaggg
    14761 cagagacggg ttgattacgg agatcaggta cgtactgttt gccgtgatcg gcgagtattt
    14821 taatctcttt tagcattgcc tttcaggtat tatctacagg tcgttgccac agtaagaaag
    14881 gttaaagctc ttgttgcata ccgggaaatc agatatctcc tggcttcgta cggcaaggac
    14941 aagggccatc cagttatgca tggagggatc cttgacctta tacatggcca gctttccgtc
    15001 actgtcagtc accgccacat ggcatatctc ccctctccat ccttctgtcg ccgagacagc
    15061 caggcagtca ggcctgaatt tcaggtcata ctgcggtctg ccggtctcct tcgcaccact
    15121 tctccaactg tcaatcagcc tgcggataat ctccaccgat tcttttactt caagggcacg
    15181 gagcatccca cgggcgagaa cgtcacccgt ttcctgcatc accgggttga tctccatctt
    15241 cctgtaatac tggaagggat gggtcgcacg catatccctg ggcaccccgc aggtgcgtgc
    15301 agccataccc accgcgccta tggaagttgc ctgcttccgc gtaacattac ctatatcctc
    15361 aaagcgcccg agaacgcttg gaagggtata aacacaaccg gtaacattgc cgtatctcct
    15421 gtcaacatct tccagaaccc tgagtatttc gaccgcggac tcctcagtga gcgggaagtt
    15481 cgatccgccg ggacgtataa ggcccttccc aaaccggttt ccacaccaaa actgggttgt
    15541 gtttattacc attgttctca gagcctcgca agccacctgt cccaactggt aagccacatc
    15601 accgcacaga gcagccgtat cacctatgtg cacggcaatt cgttcaagct ccagggcaat
    15661 gcagcgctcg gcctggagtg aaaccggagg ctcaaaacct gccagcgatt cagccagctg
    15721 tgcatgtgcc agaccgtgtg ccacggcact gtcgcctgca atgtcttccg caaggagtgc
    15781 ccgacgcaga ctgtcaccgg cagacgtgag gagcttctca ataccccggt gctggtatcc
    15841 aagctgaatc tcaagatgca gtaccctctc accgttgcat atgaaccgga agtgaccggg
    15901 ctcgatcaca cctgcatgaa tgggccccac tcccacctca tgaagctcct cgctctccat
    15961 gctgtagaat ggatagttat ccatcacact gagcctgtca gccctgtcat gcggatacct
    16021 caccggtttg agccagggat gaccctcgaa aaccacacca tgattttcat gtatctcacg
    16081 ttcaaatata tgcatcgcgt aacacgacgc cgtcaacgac ttcagcgacg tgccgtcatc
    16141 tttcttaatg tggcttagta tccttatctc cccggaaccg tcatcggcaa tgatgcagat
    16201 gaacctgaat ccttcgccgg tcctgaaaca ggagtagctc aggcaatgat aacgttcatc
    16261 tgtcagcatg gaggtgactt cttcatggaa caggtcataa ccgatcaccg ggatctcaga
    16321 aaatcctgcg ggtgacgaac cgttcctgac tactgtatac ctgttgctgc tcatgatttc
    16381 tatctgggta gcgatgagat gctggtgctg atcaggtcag taagaaaggg tggctgccag
    16441 aagcagatca taatcacgag ggccagcagg atgaactggc tgaccgtctc taccgggctg
    16501 accatgcccg gttgcagcaa ttcactgtca ttcctcggtg atgaaaagct gacatgcatt
    16561 atcctcgtgc tcatcgcata aattatatag cagagaagaa tgactgtcaa agccatgacc
    16621 agccactggc cgttgactac catcgccctg aggatcataa gttcagagat gaacattcct
    16681 gacgggggta tggcaagaat gcagatcatg cctgtcataa gcaccatagc ccctgcggga
    16741 tagagtttca tatacctgcc gctttcatcg agcctgaatg tgtgaaggat ccttgaaagc
    16801 tgtcccatct ggtaaaaaag gcttgacttg gctaaagaat ggagtactat aagcagcagg
    16861 gcggcataat agccgtatcc gccaacaccc agggcgattg caacaagtcc catattctcg
    16921 aggctcgagt atgcaagcat gcgtttcaga tgttttgcct tcagcatgta accggcagct
    16981 ataagcaacg aaagtacacc ggccaggata agtacattac tcatgaatga gagcgccgga
    17041 gtgccggaaa aaagcgtgta aacgcgatag atggcaagga atcccacatt catcagggtg
    17101 gtggagatga gcgcgctcac aggaggtggc gccacagagt gggcatcgat ggtgactgta
    17161 tgcatgggaa agagacccat cttggtgctg aatcccacca gcacaaatat gaacgctatc
    17221 ttgagataca ggggattggc gttgctgata acgtgactaa aagaggagaa ggagaggttc
    17281 ggggcatcgc cgcgtccgta gataaatccc aggaatagga ttccgaggta agccaaagca
    17341 atacccgtgg aacaaataaa tacgtatttc cacgtagcct ccagcgccat ctcagtgcgg
    17401 tcgtggtaga tgagcaccgc cacggcaagg gtggttgcct ccacgaaaat ccacagaacc
    17461 gtcatcgtgt tggccaggta cgctccggcc atgaatgtca taagtgctat cagcccggag
    17521 tgatatatat taagtctctt gacggtgtca tcacgggtat agataaagcc atggtaaacc
    17581 gtaggaagag tcagcagggt cagtactgac aacagcagca ccgccgtact gtcataggtg
    17641 aaccaggtga gttcggtgtt gcccctgtta atccaggcgt gaaccgtaat ggcaatgaga
    17701 agggcagaga acgcaagtgc aaatgccctc gtcagtcccc tgttccggga gaggagtgta
    17761 acgccgctca gcgccagtga cagtatgaag aagagcaaca gcatcatcct taatccttta
    17821 acatggtcag ttcatttgta tgcggcttga gccgaagccc gaaaaagccc aggatcagga
    17881 cacctacgaa gatgtcaaga agtatcccga tgttgataag catcggcatc tcattgccta
    17941 tggccagaga aaagaggaag accgcattct caaccacaag gaacccgatc aggtgcgaaa
    18001 taatgagctt gtgggtcaca atcagaagca acccggtcag aagggtgaac agtgctattg
    18061 caaggtatat cgaattgatg aagggattca caaggatcac ggcaagaata acactgatca
    18121 gcagtcccgc tattgaaaaa agcagcaggt aaaacccgga aacagcctgc ctgtgcacct
    18181 ttgtcacacc cgacttccgg ataatccggc tcagcaggta tggcagaagc agagtcttga
    18241 aaagcagcgt ctctgccact atgaagagca ggtttgccag gttgacctcc ttaagttcaa
    18301 acactgcaat accgcacagc agaaggccct gcagcgccac cagccttaca tacgtactca
    18361 gcctttctgc cgaggcaaag tagatgagag tcaccgcaaa gagaactata aggtagttta
    18421 ccatttcctt tcaggccagt ttttcagtca gtatcagaat aaccataaac gcgatgaggg
    18481 ctatcgagga cagagtcagg atgaacttcg gattcttgtt catcttgttc cttgccctga
    18541 atgattccgt aaatccgatt accacggcaa aaagagcctg cacagcgaaa aagagcccgg
    18601 tctgcagaat cacattccat ccggcaggta caacgagatt atatatcagc gagccataga
    18661 tggcaaattt caggtatgtg ccaatatgga tcagcgcctt atcgaagccg ctgtagtcaa
    18721 gtatcatcac ctcgtggatc atggtcagct caaggtgtgt cttcggatcg tcgacaggca
    18781 gcctgctgtt ttctatcatg gcaatctgaa ccaggagata agcccctata atactgtaaa
    18841 tcaacacata atcattcccg gtaataaaga agtggttaaa tattacggaa aatgaggtgt
    18901 atccggtgaa cattgcaaag gtggcaatca gaatgaagaa ggccggttca acaagcattg
    18961 agaagagggc ttcacggctg gctcccatcc cttcaaagct gcttccggta tcaagagctg
    19021 caattatcgt aaaaaacttt ccgagagcta gcaggtaagc gaacatgatg aaatcgccct
    19081 caaaggaaag cactgccttt tgctccgcaa agggcagaaa cagcatggct ccggtgactg
    19141 ttgccagggt gaccgacgga gctattctga aaatcaggcc ggtggtgtca ctgaatacac
    19201 tccctttcct gaacaggaca gcaatatcct tccatggctg gaatataccc gggcctttgc
    19261 gtccggaggc gatgctcttc gtccggagga ttactccggg gaaaaagaag gctgcgacag
    19321 ctatgagtac aaatcctgcc atcatcagat tattttgagc attgccagca acaatacaag
    19381 caacatgaag acaaatgcat agagaatata atgtcttatc tgaccggtct gcatcacggc
    19441 tatcctttta agcaatcccg agaccaggtc aaccggcctg tctacaaggt gtcgcttgaa
    19501 gaggtcatcg ctgtgcgaaa ggaatttcct cccatgaggg aatatctcat cctctccgat
    19561 ctcttcaatt tccttcctgg tctgcagcaa tggcttggcg atgtggttat agttgtaggc
    19621 gaacgatgtt gctgtatact gttgcttcgc tgacccggcc gtatagccgc agccccaggt
    19681 ggggccggtt tccaccacgc gccggctcag gtgatacctc cggtagagca gaagcacggc
    19741 aacagtgacg acaaatatcc ctcctgccag gctgagctga gccagattgg cggtgaaggc
    19801 tccggagacc agtggctgtg cgcccaggtt ccatatctgc ccgacaatgc caaaaaccgg
    19861 tttcaccacc agcggtgatc caagcccgat aaagatgatc atggctgcag tggccagctg
    19921 cggataaatc atggattttc ccgcctcacg ggcatgtccg gcctgatctg acctcggctc
    19981 gccaagaaac acgattccga aagccttggt gaagcagaag atagcaagtc cgccaataag
    20041 tgacaatgct gcaattgacc ccacaatgag tatggagtgg aagagattgg caccggaaag
    20101 gctgccgaac atccccatat atatcaggta ttcagagata aaaccgttga acggcggcag
    20161 tccgcagata gccagcgatc cggtcagaaa caggatggca gtccatggca tccgtttcat
    20221 caggcctccc gtttgctcca tattgcgagt atgggcagcc aggtaaacag agccggcatt
    20281 aaagaagagc agtgacttga aaagagaatg attcaggacg tggagaaggc ctcctgaata
    20341 gccaagcagg gaaagggtat gattgcctgt tgcctggcct atgactccca ggccgagacc
    20401 aattccgata atgcctatgt tctcgatgct gtgataggcg agcagccttt taaggtcatg
    20461 ctggactata gccatcatca cacccaggac tcctgatacg agagatataa tcaggataat
    20521 gacacccgtg accagcaggt ctgactgcat tgcagccagt acccttacga taccgtaaat
    20581 gcccatcttg atcatcaccc ctgacatcac ccctgagaca tgtgacgggg cagccggatg
    20641 cgcctcagga agccatgtgt gcagcggaat gaatccggcc ttgatggcaa aaccggcaaa
    20701 gaagagcagg aataccggga ggtttcggtg tctgccaaaa tattcaccga gtgcgtcgaa
    20761 gctcatcctt cctgtatcct gctgtacaat caggaatgcg acaagaatga agaacatgcc
    20821 cacatgcatc tggatgagat agctgattcc tgtcttcatg atgctcctct cttccgcatc
    20881 gaagatcacg aggaagaacg atgagagagc cattatctcc catactatca ggaaagaaag
    20941 cccgtcacgc accatcacaa ccataagcat tgaaatccac agccacaggt atgagaagta
    21001 atggattgaa aaacggagag cgttcatccg ggcacggtaa ggctcaagat accccctggc
    21061 gtagaggaac ccgacaagta cggtaaaatt gatgacaaga atgaaaaaag cagaaagact
    21121 gtcaattacc agcaccatcc tggtgcctgt tgccgcagtg aacagggata attcgcaggt
    21181 ctgcccggcg tctggaagca cacctgtaca ccatgatgtg gaaagtgcaa tccctgctgt
    21241 caggaggccc agggtaaagt aatacttcag ccattcaggc actgcaaaga cgaacagtga
    21301 gagaagcaag atgaccagaa aggctgttga tccaaacatt aatccggcaa tttgcccgca
    21361 aatgtaaaaa atatattatc cggttatact ttaaaccgga ttaagtcaga actgaccggt
    21421 tatatctcca gctggttgtt gccgcccttg aggcctatca ccattgtatt tccgcctgcc
    21481 gggatgttgg ggtcaaaagg aagatttgcc ttgtatgatc cgcaggtaaa gtccggtaca
    21541 tcaattgatg acgacctgtt tgccactgac cattcgctga gaggggtgat aacgctccag
    21601 agggcggcgt catagacatc ctggtcgaga ggcagtccgt ttcgcaggca gtctatcagc
    21661 ctccagtcca tgatgaagtc catcccgccg tgaccaccta tgacctttgc catctctccc
    21721 acccttttaa caatctcagg tgtgtactga gcctcaaggg cagcctgatc ctcaggtttc
    21781 agccatgaat ggccgagggc tatccttgcc ggtgatggcc acttctgggc aaatcctttg
    21841 gtgccgctga cgagatgtat cctcgaatat ggcctcgggc ttgaaacgtc atgctggatc
    21901 ataatgctcc ttccgagtgc cgtctttacc agagtggtgt tcatgttgcc acggtaactc
    21961 ttcccggcaa atggagcata aaacgggtcc tcctttgcca gttgttccag ctccggtccc
    22021 atggcgaaat catttgatga gagtgatgtc aggtattcca tcctgtcacc cctgttgatg
    22081 ttcattatct gacagaccgg tcccaggcca tgggtcgggt aaaggtcgcc gttgcgcaca
    22141 ttttccttca gcctccacat gtcagcataa ccgttcttat cataggtgaa gtcccatcgc
    22201 aggtcatgga tataagcccc ctccccgtgc actatgtcgc caaagagtcc ctggcgtgcc
    22261 atgttaagtg tcagcagctc aaaaaagtca tagcagcagt tttcaagctg catgcagtgt
    22321 ttctttgtcc gctcggatac ctcaacaagt tgccagcact cctccacagt ctttgcagcg
    22381 ggtacttcca ccgccacatg cttgcctgct tccatggcat agacagccat aggggtgtgc
    22441 catgcccagg gtgtgcaaat gtaaacaagg tcgatgtcgg ggtcatcaca caatcctttc
    22501 catgcttcct cgctgccgct gaagcccttt gcctgtggca gcccgaactt tgtgagcatc
    22561 gcctgcgcct tttcaaccct gtcgggatac ttgtcgcaga gcgaagctat ctctacccct
    22621 tctatgtgtg ccatccgctc gacggcaccc ggacccctca ttccgaggcc gatgaaacct
    22681 atcctgattt ttcccagcgg cggagcagca tagcctgaca tgttgaagct ctggttgtgt
    22741 acggccattg ctgccgccct gacctctgcc atggcttcgg ttgatttttc tcggcggaca
    22801 catgaggatg tactgacagc cgtcagagtt cctgctccgg caactgcagc agtgcggaga
    22861 aaattccttc tgttggtttt catggttagg taagtttcga aatggattat gtatgcaata
    22921 tacaatgttt ccgattacaa tattatgtcc gcggctggaa gtcaaatggc ttaaaggggg
    22981 cacccgggcc gggcttctcg gaatgagccg gacagccact tctcagtggc cttcctgttc
    23041 cggcggtctc tttctcttcc tgatgagatc acctgccact gatccggcca gataacctag
    23101 cacggcagcc catggtatca tcaggtacca gtttgacttg atggagcatg ttccggagac
    23161 gcatccgaca tatttccagt atagaaatcc tcctgctgcc cctgcaaccg tgccgattat
    23221 ttccggaaga tatttcctga gaagatcttt tttcatttta tgctgttttc tttaataacc
    23281 cttatgaaca ggtcacgttc cgctatttgt tccttttccc aggaaagttc ttctgcagca
    23341 ctgagggcat tccccttcag atctgagagt aactgctggt catccctgag caatgaaagt
    23401 gcaccggcaa ttgcccgggg cgtcagctca tcaagcacca ggccgcatct gtacttcgcg
    23461 attatggctg acacctcagg cagcggggaa acgatcacag gcactcctgc acctatggta
    23521 tcgaatagtt tattgggcag actgaagcgc tgattggcac aggtgtctgc gtcgattgat
    23581 aaacctgcat cacagcactt tataaagccc atcatctctt cccatctcat tcttggcagg
    23641 aagatgactt tgtgatcaat gcgctggttc tgcacctgtt gcctggcctc ctcaattctt
    23701 ttgccggtac caatgataac aagcttcaca ttttcaagaa gcgtcattgc ctcaagcagt
    23761 tctgacacac ccctgcccgg attatcaccg gagccatcct gaaataccac aagcaggtca
    23821 tctgccgagg cagccagatt gcggcgctga tacggcagga tattcaatac aggcatggga
    23881 aggtttctga tcacaaccgg ttcacatccg tactcctggc tgtaaatttc tgctaatgaa
    23941 tcagataccg tcatcatgta acgcaccttg ggcagcagcc tgcgctcagc gttcttccag
    24001 agagagcgct tgaactttct ttcagtgagg ctgtattgcc cggtaaaata ttcatgtgca
    24061 tcgtacacaa gcggtctcct gaataaacgc gaaaccatat gacacggaat cattgtgtca
    24121 agatcattgg cgatatacaa gtcagctctc ctcagcaata aaagcctgaa caataaggcc
    24181 acattgaacc ataaatacat catcggcccc ttcctgaaag gcaccctgat cattgccacg
    24241 gctgctgaag gaagagaaag aggcaaagag ccttccatgt gcctcccgat gagcgtcacg
    24301 ctgaagccca tcgatgaaag cacagctgaa tgcttctgaa cccgcatatc agttgacaga
    24361 tcactgatga ctgcaactat tgccctcata ttctgtccgt tacacaaaac accatttata
    24421 caaatgctgt taaaattggt gtaagataca aagaaaatta tagctatgtt tgtgcattgt
    24481 ttgaatgctt aatggagaga gctcccgcca aaagaatagt ccatcttgtg agtttcaaca
    24541 tcccttaccc tcctgactac ggtggcataa tggatgtttt ctacaagatc tccgccctta
    24601 agagacaggg tgtcggggta atactccact gtttcagcta tgggcgcggc cgttcaagaa
    24661 cacttgaacg cgaatgcctc agggtacatt actacaggag ggagctgaat ctttttcacc
    24721 tcttccgtaa ggagccgttc atcgtggttt cacgcaggaa cgatatgctc ctcaaagacc
    24781 ttctgaccga cagccacccg ataattttcg aagggttgca tacaacccat ttcctgggcc
    24841 ataaggcctt ggaaggaagg atcacaatgg tgcggactca taacatcgag catgtctact
    24901 accgcaacct ggcctcgaat gagaaaaatc ccttcaggaa gctttattac agcactgaag
    24961 ccaggaagct cgagcggtac gaaccgaagc tcgcaggtgc cacatggctg ctgtcaattt
    25021 ctccgggcga cacagaatat ttcagttcaa agtatggcaa tgcaatgttt gtcggtcctt
    25081 tccatccggc agacggatca tttcctggca acggcagggg tgattatgta ctgatgcatg
    25141 gtgatttctc cactgccgag aatgatgctg ctgcactgta tattctgaag gaggtggcct
    25201 ctcactggaa ataccggact gtcattgccg gcaagagacc gtcggcagaa ctgtcggacg
    25261 cagcctcggc attaaggcat gtgaaactgg ttccaaaccc ttcactgacc cagatgaatg
    25321 aactcatcgc aaatgctcag gtatgccttc tgaacgccac ccagcctacc ggcatgaaac
    25381 tgaagctcat taatgcgctc accaccggcc gtcacgtcgt ggcctcacct gcaatcgtat
    25441 cagggacagg acttggagaa ctatgccatg tggctatcaa gccaggggaa tggataagtc
    25501 tgactgacag tctgatgaaa gaggatttta ccggtgccat gagggccagg cgcaacctcc
    25561 ttctgaagga agtggctgac aatgacaaca atgcaaagaa aatagtcgag acaatcgaca
    25621 gttacaatcc tgaattatag gcttatccct actggtatat ccttctcctg ttaagttcgc
    25681 gcctgtaggc agcggcatac ctgttgtgct cggccagcgt tctggaaaaa tggtgataac
    25741 ctgatccgtc atacctcgca acgaagaata gatattcatg tttttccgca tttaaaacag
    25801 catcaaggcc tgagcgtgac ggacaccgta tcggtcccgg tggcaaacct gcatacttat
    25861 atgtgttgta gggtgagtca acttcaaggt gcctgttgag gatgcgtctg agtgtgaagt
    25921 cattgactgc gaacttcacc gtcggatcag cctgaagcgg cattccgagg cgaagcctgt
    25981 tgagataaac cccggcaatt cgtgactttt catcctctcg cgaggcttcc tcgtcaacga
    26041 tggaggccag aacagacacc tccggcggtg taagccccag tgattcagcc ttcgcagtcc
    26101 ggtcatcatt ccagtagtcg cggtactcct tcagcatgcg ccggtaaaaa ccgcgggggt
    26161 caactgacca gtacaattca tatgtatcag gaatgaacac tgctatcagt gttccgcggg
    26221 taaacccgtc agctgagtaa ttggactcat catggaagaa ttccgccagg gatgctgaat
    26281 ctgcctctat ctgccctcct gttttgcctg caagctcctc aagggttctt atattgttga
    26341 acgtgacgtt tacaggggtc tggcggcccg agcgcagcat gttgacaagg ctgttgttgc
    26401 tcatcccggg ttcaatgaca tatgaaccag gcttgactgt ttttccgaaa gacttgagcc
    26461 gcgaggtgat tatgaagctt tccctgctcc gcaggagatc agctctctcc agcgtatcag
    26521 tcagagccct gaaatctgac ccggtgggta gatatatcct gaccggttca tcatgcttca
    26581 cattctccct gagcactata acccacagaa gcagtaccag cacaaaaccg gcagcaagga
    26641 gagtgtaaaa aagtgtcaga agtatctttc tcatcgggta cgaatttaat agagaggccc
    26701 gaatatccgt gccagtgact ccctgtatct ctccctcctg tttcgttttg accactcctg
    26761 aatggttacc tcggcacagt tcttaaggtc ctcggcgaag agcaccccca ggcggagtgc
    26821 aaaatcttca tcataaatca gggcggtaac ctcgaagtca aggtcgaaac tccggacatc
    26881 aacgttggga gaccccactg aggaaaagat gctgtcaacc agcaggtatt tggaatggat
    26941 gaacccgttg cggtacagga agatcctgac acccgcctcg agcaactcca tgatatagga
    27001 acgggtgttc cagtttgcaa taatcgaatc tgaattttca ggaaatatca ttctcacatc
    27061 aacaccgctc agggcagcgg tcttcagggc tgtcagcaga ctctcgtcag gactgaagta
    27121 cggtgaagtc aggtatattg acgattttgc cgtagcaatt gcagaaaaat aaacctgcat
    27181 tatcgtggcc cagtcagtat caggtccgct ggaggcgacc tggaccaggc acctgttgcc
    27241 tgtgcggacc acatgctcaa gtgaaccgtc cggcggcagt tcctcgccgc tcacaaaatc
    27301 ccagtcggcc aggaaaaccc tgtcaagtgc tgcaacggct tcaccctcaa ccctcagatg
    27361 ggtatcaacc cagggtccaa ggtgaggcag gccgtgcaga tacttgtctg caatattcag
    27421 cccgcccaca aaaccaacct tcccgtcaac caccagtatt ttcctgtggt tccggtagtt
    27481 gatcttgctt gagaagctgg ggaaacgaac cggaaggaac ggaaatatct gcaccccggc
    27541 atcacgcatc atgtttataa aaggtttgcg gatgttccag ctgccgacat cgtcatagat
    27601 gaccctgacc tttacccctt cccttgcctt gcgcatcatc agctcactga attccatccc
    27661 gatggtgtca ggttcaaaac ggtagaactc aaggtgtata aagccggtgg ccgatgccac
    27721 ggcgtcaagc atggcgggaa acgtctggct gccgtttacc agcaggtcaa tctggttgtc
    27781 aagggtaagc agtgaccggt tattcctgag catcaggtgt atgagatgac tcttggacct
    27841 gattgcatca ctctcgccgg ccagcaccga tgagagcagc ctgacctggc gatcggcatg
    27901 gtcggtcagc ctggcgctgt cagccatgct cttccgggta tacatcttct gcttccggta
    27961 gttcttgccg aaaaagaaat agacgatgat ccccacaacg ggaacaaaca ggatcaccag
    28021 cagccatgaa agggtcttga gaggattgcg gttctcaaag acaatcatca ggcaaatggc
    28081 aaaaacagct atcagataga taactgtgag gacatttctg ataatataga tagtgttccc
    28141 tgccaggatc atcctgcttc cgttcacata ctaccgtaaa tataaaaaaa gaactcttcc
    28201 ggcagactgc ggaagagttc ttttatatgt cacggatatc agaagatcag tcccacctgc
    28261 aaaagcaatg ctcctgtgcg gtcatcaagc tggaagttct ggtcaccgat ttctatctcc
    28321 tcaccgaact ggttgaggtt cccttcatac ctcaggtcaa aggtgagcct tttgaaaagg
    28381 tcaaatccaa gacctgcctg gtatccgaag gttgcccttt tataggtggc tccctgtatt
    28441 atctccttcg ggtcagatat catgactgaa gctgctggac cagcattcag cctgagcgga
    28501 ccaaccttta ccccgagaag aactggaatg ttcagtttgt tgaacttctg gttgtagatc
    28561 accggagcgc cgccgccttc cacatcctca tatgataccg agttggttgc tgtggcaaga
    28621 agcagttcag gctgaatata gaatcctgaa aaggtggctc tcatgaatac tcccccctgg
    28681 aatccccatt cagcatcctt cgcattctga agaatcacct cgaaatcggt acctttaaag
    28741 gtctgatcgg taaaggtgaa gtcggtgctt gccccggcct taagaccgaa tttgacctgt
    28801 gaaaacaacg gtgcggcaag aaccagtgca agaataatga caatgaactt tttcatttct
    28861 ctcagttttg gttaatagaa cagtagtagt tattgttctg ataaacgcaa tattcaggcc
    28921 aaaggttgca ccggcaaccg ggaaagagcc tgttttttta cttttttgcc agctctgaga
    28981 atataactga cttcattttg tcaaactctt cacgctcgaa aaggcgggtc agaaagatta
    29041 ttttgccgtt gcggtcaatg atgatattcc gggtaactcc ggcctgtttc tcggcgtaca
    29101 acccgaaaat gtcagccccc gggtcaagtg ccagagggta ggtgattttc atatcagcgg
    29161 caaatttcct gaccgttgcc tcaggctcgt cacggtcaat accgataacc acaagcccct
    29221 gcttcctgcc cggcatccat atctcctcct cgatgaatgg catctcctta cggcatacgc
    29281 tgcaccagct tgccgtgaac tggagcatca ccacctttcc cttcaggtct gacagcttat
    29341 aactcttccc gccagcttca ttaattgcaa aatcaggcgc actgtcaccc acctttacaa
    29401 gatagttgtt ctcatatacc ttttgcccgt gagataaagc agcaaccacc aacagggcaa
    29461 tcaccatcaa aactgttctt ttcatcgctc tatattaaaa gtactaagat atatgatttc
    29521 tcggtttact ttggttatcg ggacagggta attatgtctg cccggcaact ccggagaatg
    29581 ctgctcctcc ctatttcaga agctcatagg aaagacctgc ctgaaacgat acattcttat
    29641 ccagataggg atttcccagc aaccagcctt ttgtttttcc tgataccgaa acaaatcccg
    29701 agaaactgtc attgaacaaa taccttgtcc tgattcctgc ataccctccg gtaaccatgg
    29761 aatctgagaa gaatgattca ggctggctcc atacattcaa cctgagctcc gaccgtacct
    29821 tgcctgagac acccaggttg tacagcgaca gaccagctcc aagacccgtg tcacttttgt
    29881 tgctgtacct gtgagcattt accatgacac cgaatcttct gaacctgacc ggaacaaaga
    29941 gtgcaatgtc attgccaaaa tgagtgggag aatactgggt gaagaaattg aatgagaaac
    30001 cctcactgaa cttaattctg tcgataaaga atatgccggg attaagaaaa ttaagaaggg
    30061 aaaggtcctt ctgcctggta agataatcct gcccttcgga cgagagatca gaaaagccga
    30121 ttcgcctgtt taccccttca cctccgggga acggatctct ttcagtataa ggcaggccgg
    30181 gattgaacat gtcgtgtatc caggcattaa ggtcagcccc tgcataatcc ctctcagcag
    30241 gattggtgtt ctcatgtggc ggagccagca cttttacaga gtcgctggcc ggacttgcag
    30301 agaatctgaa gtaattatac acataccagg cattgtacaa taacaaagag gctttgggca
    30361 aagacctttc gaaatagaat tcactgatgg caatccgttc attcagattg atttcatatt
    30421 gcactccggc gacataagag tagagcaggt tattaattcc ggtggtcttc aggtcctgca
    30481 gagtcatatc cgaaatcccg taaacagtcc cgtcccagcg gctgaggaac cagttaccgt
    30541 tcttcgaaga gactccgctg gtgccgagga cagcccggtg atattcctca tgagcccata
    30601 ctccgagcgg aatcggaagt tcactgccat attttgaaaa tccaagtccc agagcatact
    30661 tgaatgcact attggtaaac ctgccgaatc ttgtttccgg ctttgacctg gaagtgaaaa
    30721 ttgcatctcc gagttcatca attccccagt atgcgaggtc atacatgccg gaggagaatt
    30781 caagtgcctg gttcatggat ggatacctgt atggcaggtc aatgttctgt ggcaggtcaa
    30841 tcaaaggaat gtcaagccgc agcctgtatt caggattgtc ctgacctgat gagagtgctg
    30901 atgataatag aaagactacc atcaaaaagt tttgacttga tttcatagtg cagattttaa
    30961 ggatttgaag aagaacagaa ataaatcctt attgttcact ccgggccgga gtacccggct
    31021 ccgggaagcg gcctgtcccg gagctgttga aggtacatgc cacataatca ccggcagcat
    31081 aaataatggg cgtctgagat atatcacaac gctttttcag acctcctcct catcaatgat
    31141 tgcctccagg taaacactgc tttttatcag ctcaaggtat ccctcatcag actccctgaa
    31201 tccaaattca aaacagtaag accagcgtaa accctccctg gtttcgacaa gatgcgtaaa
    31261 gaaggtgaaa ttgtagccct gggctataag tgcatcacgg tgaatgcggt gatgaccgtc
    31321 cccgtagaga tctgacagga ttttcctgtt ccgcctgagc tgcgcatgca cattgcgcac
    31381 gtagttgttc tgatcacggt tgagacggtt gttgtagttg ttccggcact ggtcagaaca
    31441 aaatttctta tcagaccgtc cgtggagcct ggctccgcag tcaagacata ccctttccac
    31501 agcgagaatg ttttcataaa ggtaaaataa ttccatggaa cgggcatact gaaaactgca
    31561 catcattctg attactattc tgtaacccgt ttacaaacgg atataaacga ctgctaaatg
    31621 tatagaagtg attaacaacc ggatcaaatg ccggcgaggg tgcacatttg tatcgttaac
    31681 agctttatta actaaaaacg aatgtcatga atgcattgag aaacaaagtt cagctgatcg
    31741 gtcgcctggg acaggatccg gaaatcaaaa cgcttgagag cggaaagaag gttgcccgct
    31801 tcaacctcgc caccaatgag agctacaaaa actctgaagg tgcaaagatc gatgagacca
    31861 cctggcacag tatcgtggta tggaacggtc ttgccgagct tacctcgaag tatctcacaa
    31921 agggtaagga agtatgcatc gaggggcgca taagctatcg ctcctatact gacaaaaacg
    31981 gaatccagag gaatgttacg gaaatcgtgg cctcggacat ggtaatgctt ggttccggag
    32041 ccaggccgag aggactgatc ggtgacgagc caggggagga catggccgat gatccggcag
    32101 cagggctggc tgatgaaccg gttgaggccg gggcttccac ccaccggggc agcagggtca
    32161 ggatgaagtg aatcaggcgc ttcgctttat cccgggcgca agaccggaat gaagtgaggc
    32221 cgtccgggat ttgttaccag atcaaagcca ttcatggagg ggggagagag tgtggagagg
    32281 gtgtctcgcc ggaaggcccc ggcaaagcgc cggggcctgt ttgcctggtt gccttttcta
    32341 tctgcaggca ggtgcacgcg tcacctggca gctgccgcat ccctcccggc acgcagtgcc
    32401 ttcagattga cctcaacaac ctcttctccc ttgcggccga acatcttcct gaccgcatct
    32461 tcgagcgccg tgaagggcat cgagatgaat gacgaagcag cacccaggat gaccatgttg
    32521 cctgatctgg ctgatcccag gtcacgtgca atgccgtctg catcaagcac aatggtgtta
    32581 cccgccctgt taagttcagc cagcacctct cctacctccg ggtaatcgga gatattcacg
    32641 aagggaaccg aattggtaac cagccatccg ctctctgaga gccatggcag ataacgaagt
    32701 gcctccatgg gttcaacact tatgatgaga tctgcacctc cgtgcggtat aagatctgaa
    32761 aaaatctcgc tgtcggagag acgcaggtgc gactgcacgt cgcccccgcg ctggctcata
    32821 ccatgtacct ccgcctgctt gaggaagagg ttctgttcaa cggctgcaag ccctatcgtg
    32881 gctgcaatgg agagaatacc ctgtcctccc actcctgcaa gtataatatc acatttcata
    32941 tcattccctt gatttgtttt tctcagccgc ttcggccttt ttgttgcgtg atgccgtctg
    33001 gatgcactcc cgtcttgcca gtatcaccga gataccgtga taattgagtt cagcgcggat
    33061 aacggctttg ttctcctcat gattctttct cagaggcact atcaccttca ggtgatcagg
    33121 atcaacaccc agtcccaggc agatgctctc aagcctgtca gtggcctgcg atttctgccc
    33181 cccggtcatc ccggtggtgg agttgtcaga gataatcagt gttaccggtg aattcttgat
    33241 caccgcatca agcagtcctg tcatgcctga atgggtaaat gtggagtcac ctatcacggc
    33301 cactgccgga aacagaccgg catctgcagc tccgattgcc attgtcaccg aggctcccat
    33361 atcaacacac gaattgacca tgttgtatgg tggcagggcg ccgagggtat aacagccgat
    33421 atctgaaaag acgcgtccgg gcccggcttc tgccattacc tctgacaacg cctggtacag
    33481 gtcggcatgt ccgcagccct tgcagaacga cgggggacgc tgacgcacca gcgagggaac
    33541 ctccctgccc tgaccggccc tgagacccag ggcactggca acaattgacg gattaagctc
    33601 cccgtcgcgg ggtaccgtac cgtccatcct gcccagaact ttgacgtcac ggtcaagaag
    33661 accccttagc tgctcctcga ttatcggata accctcttcc agcacaagaa ccctgtcaca
    33721 gttttccacc aatgctgcaa ccttttccct tttgagggga taagcaccca ctgatagaac
    33781 gtgataatct cctgctcggt cagcaatgtt ttccatcagg taattgtaag caatcccgca
    33841 ggtaatgacc cctgtgcccc tgccgtttcc ggatttcagc ctgttgagcc cgtcaacagc
    33901 atcgtcagtg cgcagtttct cataaaccgc tgtaagagcc ctgtactgct tcctggctat
    33961 cgctggaaga agcacaaact gccgcggatt cgaaggcagg gcaacactgt tctgagcaat
    34021 cttttccctc cgtgcaacag atgaccttga atgtgccagc ctggttgtaa tcctgaacaa
    34081 cacaggaagc ctgtacttct ccgagagggc aaaggcttca aagatcatgt cataggcctc
    34141 ctgctggtta cgcggctcca ttaccggaac cagggcaaac ttgccataaa agcgcgagtc
    34201 ctgctcattc tgggatgaat gcattgacgg atcatctgct gatacaacca ccagcccgcc
    34261 gttgactccc gtcaccgccg agttgatgaa cgcatcagcc gccacattca accctacatg
    34321 cttcattgca accatcgccc tcttgcctgc atatgacatc cccagggcag cctccatggc
    34381 tgtcttctca tttgacgacc attcacggtg aacaccgtcc gcagcagcct gcggcgaagc
    34441 ctggatgtac tccataatct cagttgacgg agtacccgga taagcataca ttccgctcag
    34501 gcctgcatcc agggcagcct gcgcaatagc ctcatctcca agcaataaga gtttatgcat
    34561 aattatttag ctttagtttg tttacggtta cggtgacagt aaagatgatt aaagttaagt
    34621 taaaatcaag aagtaaatgt cattattgtt tactttacat cacaaaatta tctgtctttg
    34681 aagaagtttt tggtgatttg catcatgctg ttgttctcgt tcccgtggct gggtgcccag
    34741 accggagaga gaaaatatat ccagatcgac ggcatgactg cagatgaaag gggattcatt
    34801 gttcctgatg taagcatata ttcgaagcac ctgcgccggg gagctgcaag tgatcaccgg
    34861 ggtatcttct ccattataag cgctcccggt gacactgtat tcttcagctc cattggctat
    34921 aaaccgacac tgctgaccat ccccgtaact gccacaggcg accattacat aacagatgtg
    34981 ataatggtca ccgatacaat ccagatcgga gaggtactgg tccttccgtg gaagacctat
    35041 tcagagttca agagggctgt tctcgaagat aaacccaggg accccctgat ggagaacatg
    35101 gaatataacc ttgccatggt ccagcagcag atctggagtg acatgcgccc cacacccggg
    35161 gaggcatacc actacaccat gcagcaaatg tctgaccagc tctacaccag gggccaatct
    35221 cccgcaaaca acctgctcaa tccatttgcc tggcagaagt ttgtccaggg agtcaaaagc
    35281 ggaatgctga agaacgaacc ctcaaaggcc gtcaaggtta agaagcacaa aagccgcaag
    35341 aaaaaaaaga gtgactgatg gtcaggtctt cagccataag actgcttaca gtatccttat
    35401 tcctctcaat catccccgcc ggcttcctta acggacagga gaaagtcatc tccggcattg
    35461 tcacagatac tgcaggtgtg gcggttgcag gtgctacggt attcattcct tccgtggaaa
    35521 ggggaacatt atgcggaaat gacggttcgt tccggcttgc cttcaccccg ggagaatctg
    35581 agagcctgac ggttatcttc tcgtgtgtcg gatacagcgc tgacactgtc accctgaagg
    35641 cagatggcac ccttgatggc attcgtgtgg tactcatccc tgcaacgaca atcatcgagg
    35701 gtgttgaggt aagagccagc cgtcctgaca atgtcacggt gataggcata cctgctgtgg
    35761 caggtgcagt tataccatcg gtaagcggag gagtggaatc agcggttgcc accctgccgg
    35821 gagtcagcgc cttcagtgag ctgagctcag gctattcagt caggggtggc agctacgacg
    35881 aaaaccttgt ctaccttaat gacgttgaga tttaccgtcc gtatctcctc cgtaccggtc
    35941 agcaggaagg gcttagcagg atcaatcccg atctcactgc ctcggtaaat ttctctccgg
    36001 gaggattcag cgctgcctac ggtgaccgca tgtcgtcagt gcttgacata acttaccggg
    36061 agcctgaatc ccgtgaggga tcggtctcgc tgagtctgct cacctcatcg gtgcatgccg
    36121 gggcccgcag caatgacaac aggttctggt tcatcgccgg ggcccgctac cggtcaaatg
    36181 ccatccttct gggcggcctt gatacccgcg gcgcctaccg tccccatttt gcagatatcc
    36241 aggctatagg cggattccgg cctgacagcc gcacgggaat caccctgact ctatggggct
    36301 cactaaaccg ttatacattc ttgccgagaa gccagacaac cacatttggt actgtcgaga
    36361 acgcataccg tctttatgcc tttttcgaag ggggtgaacg tgacagatac aacagcggcg
    36421 gcggagctct cacccttgaa cggaaacaca ctgaccgcct gttgactaag ctgatcatct
    36481 catccagcca cgcgggtgag tatgagagct atgacataag gggtgcatac agcctgtcgg
    36541 ccctcgacat gaatgtggga agtgaaaaca atccggatac gctgctcaat gcaggcgccg
    36601 gcagctggct gtcgcacgcc cgtaactcct tcaggagtct catcgcggga gtctcatacc
    36661 gcggttcact ctcctcgggg aaaaacggcc ttgagtgggg ccttgccgcc aggcagcgca
    36721 gctatgactc tttccggaat gagtggctca gggttgactc tgccggttat acaccaggcc
    36781 aaccaggtga tgggctggct ctgacttccc gcactgtcct cgggaatgag acccgcagcc
    36841 tcaccagtga gggatggctc atcagccgca ggcgctttgc cgtggccggg cgggcgtgcg
    36901 acctcaacgc cgggatcaga gcctcatatg atacgtacag ttccgaaata cttatctcac
    36961 cacgcgtatc actgcgcagt gagattaccc ggcaagtctc cctttacctt gctgccggcg
    37021 catatcacca gcctcccgaa ggcagggaac tgatccgtga agatggtgaa agcggatctt
    37081 acccaaaggc acagcgttct taccacctga cggccggagt tcgctatgac ttcacagcct
    37141 gggaaagacc attcagattc accgctgaag cctggggcaa actgtttgac aggcttattc
    37201 catataagtc agacaatgtc aggcttattt actacggcgg caatattgcc gaaggatact
    37261 ccgcaggaat tgacatgcgt gtcaatggtg aatttgtgcc gggagtggaa tcatggttca
    37321 gcatgtcact tatgaaatcc gaaatacaga tacctgagct gtctaccggc tggtatccgg
    37381 ccccgttcga ccagaggatg cacttcagca tcttcttcca ggattacctc ccgggccatc
    37441 ctgacttcag ggcacatatc aatattgcct tcggtaccgg aatcccgacc tcccctcctg
    37501 gtcatgaaca gtgggatata tggttcagga tgccacccta caggcgcatt gatatcggct
    37561 tttcgaaagt cctattgggt gccggaagag ccaacaggga ttcgttcatg aaagagcttg
    37621 tggcaggtgt tgagatattc aaccttgccg atataaggaa cacaatctca tatacatgga
    37681 taaggactgt cagaaacagc cagggacaga cacgggaata tgctgttccg aattatctca
    37741 caagaagaag cctgaacctg aaactgacag cacaattctg acgtataaag aggtcagagt
    37801 gactttttta ctaatttcgt accctgatga accggtttga gatcacattt acggcagtga
    37861 tggccacgat cataatacac ctgactgtgg ctgtgatctt tatgtctgta aaaatctcag
    37921 cactcaggcg tgagatcgcc gccgagatca tactgtcaat agatgaggag atgccggatc
    37981 ctgcaatgga gaaggccatt gagcttgcaa agagcgacca gttccagggc atcagccctg
    38041 aggagatggt aaacataatc aagaatattg ctgacaagcc tctggacatt gatcccaagg
    38101 agtatgagga catggtcaag gagg
//
LOCUS       DAOPXU010000016        37044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_1021_length_37044_cov_12.205223, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000016 DAOPXU010000000
VERSION     DAOPXU010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 37044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37044
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1021_length_37044_cov_12.205223"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..871
                     /locus_tag="PKK03_02790"
     CDS             95..871
                     /locus_tag="PKK03_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNX83350.1"
                     /translation="MAEVTFNDIIKPENLAYRKSALMTDNVMHYCPGCGHGTAHQVLA
                     EVLEEENLQERTVGITPVGCSVLMYNYIDVDFQEAAHGRAPALATAVKRLMPDKFVFT
                     YQGDGDLAAIGTAETIHTCNRGENILIIFINNGIYGMTGGQMAPTTLPGMKSSTSPYG
                     RDVSMMGSPIKMADIVALLPGTCYVTRQSVNTAGGVRKFKKALKKAVQYQNEKRGTCF
                     IEVVSNCPSGWKMTPVESNKWMEENMFPFYPLGDIKVPAE"
     gene            944..1486
                     /locus_tag="PKK03_02795"
     CDS             944..1486
                     /locus_tag="PKK03_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNX83351.1"
                     /translation="MTEEIIIAGFGGQGVLSMGKIMAYSGMMQDMEVSWMPSYGPEMR
                     GGTANVNVILSDERISSPILRSFDTAIILNQQSMDKFEKSVKPGGTLIYDGNGITRHP
                     ERKDITVYRIDAAEEASKMKMQKVFNMIVLGGFMKVKPILKTEYVLKGLKKSLPERYH
                     NLIPLNEKAIERGMEIINKV"
     gene            complement(1585..2178)
                     /gene="nadD"
                     /locus_tag="PKK03_02800"
     CDS             complement(1585..2178)
                     /gene="nadD"
                     /locus_tag="PKK03_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNX83352.1"
                     /translation="MTETGLFFGSFNPVHIGHMAIANYITEYSFLKEVWFIVSPHNPL
                     KVKESLLSERDRFYMTQLAIGDDARFRASDIEFTLPRPSYTIDTLAHLTEKYPSRRFT
                     LIMGEDNLTSLHKWKNAEMLVKQYPIVVYPRLYPGRRSSKRLQEILAMASVQEIDAPI
                     MEISGTFIRSAIREGKDVSWFVPAPVWKYIREMHFYE"
     gene            complement(2175..2756)
                     /gene="gmk"
                     /locus_tag="PKK03_02805"
     CDS             complement(2175..2756)
                     /gene="gmk"
                     /locus_tag="PKK03_02805"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNX83353.1"
                     /translation="MRPADSKKVLIISAPSGAGKSTLVSHLLESGFPLLFSVSATSRK
                     PRGNETDGREYYFITAAEFRRRIRNDEFIEWQEVYKNHYYGTLRREIERIRDEGKIPL
                     FDVDVMGGINLKKIFGDDALSVFIMPPSVEELRRRLVLRGTDTPEQIAMRVEKASSEI
                     LLAGHFDTVVINDNLEKACIAIVNIVRDFIDRP"
     gene            complement(2761..3636)
                     /locus_tag="PKK03_02810"
     CDS             complement(2761..3636)
                     /locus_tag="PKK03_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNX83354.1"
                     /translation="MIRSMTGYGKAMLETPQRKITVEIKSLNSKQADINTRLPWICKE
                     KELDIRNLVIRKLERGKIDLSVSFDAMESEQVPVINRNNVKSYFSQLKEISGELGISD
                     DAGLLAIVMRLPETLRTEKAELSDEEWTRISGLTDEALTMTDLYRLEEGKAMEKDLCK
                     SVAAILSYLENLGAVEGDRISRMREKLTTSLAEAVGSENVDRNRFEQELIFYLEKLDI
                     NEEKVRLRKHCDYFLETMKGEGNHGKMLNFISQEMGREINTIGSKANDAAMQKLVVMM
                     KDELEKIKELTLNIL"
     gene            complement(3716..5095)
                     /locus_tag="PKK03_02815"
     CDS             complement(3716..5095)
                     /locus_tag="PKK03_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNX83355.1"
                     /translation="MILPYCEPYKIKTVETIRQSTRQEREEWIRNASYNLFNLRSEQV
                     FIDLLTDSGTGAMSDKQWGAIMTGDESYAGASSYYTLKETIRDITGFEHFLPTHQGRA
                     AENVLFSVLIKEGDVIPGNAHFDTTKGHIEFRKAKAVDCTVAYARDIRVVDPFKGNAD
                     LERLEQVLKETPREKLGCIVMTVTNNTAGGQPVSMANLRGVRELADRYGVLVVMDSAR
                     FAENAYFIKTREEGYSGKSIREIVREMFSYADAMTMSSKKDAIVNMGGFIAMKDRELF
                     RKASTFNIMFEGYITYGGMSGRDMNALAQGLREGTEFDYLEARICQVTRLGEKIHEAG
                     IPVQVPIGGHAVFIDAGRFFPHIPASEFPAQRLAVEIYLEGGIRGTEIGTLLADRDPV
                     TRENRYPELELVRLAIPRRVYTDNHMDYVAAAVQNVWERRDLVRRGLAIRWEAPIMRH
                     FTVELDLLG"
     gene            5367..7277
                     /locus_tag="PKK03_02820"
     CDS             5367..7277
                     /locus_tag="PKK03_02820"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HNX83356.1"
                     /translation="MKPGTIKILLLFLCLAATPVLNAQVVDAPAIPQVTAPQTDTLAV
                     TQPAPRQIKAAPVPGINGPQDVKLDRDESLRFLERFSSSPAMWKRSNDPLREAIRNLL
                     WLASRPPADTVISYLSDYPFDRIKVPAESYYLFDSIRIILPVLQPDSTHADSVAAARA
                     DEMFIRTGNKVEKVRLSPDSRPVMKNDTLKLNDSVYVLMKEFIPAQLPHGTNDTIVLV
                     ITDTLPEVSLARTDFPFRYLRNPYVSDSLEAAVSSLIGYLEQRDSTRIRFTSDGSRGT
                     DVWLNSLSDNLVRFWLPDGQGDSVTVWIGNPVRNTLSLRAEEGVLFRKQAWHDRYVDT
                     RVNVTTAQEEALRRVALSRIKPDLWKFKTDISYLLSQGVVSNWAKGGENNISSVLDIT
                     TALNNNSKLSKVISASTGRFALGLQASGKYGGIRKNLDILEINSKINHKAFGKFDLSG
                     IFQFKSQFLPGYTYPNDTTSLLVSRFFNPATFILGYGLEYKPDKNTSVSFSPFSYKGT
                     FVPDTANINQTKYGIAADRRSKNELGAYLTINSKLVLFEKVNMTNRLQLFSNFLSKPQ
                     NIDVEWEMISTMPLNWFTDLRVNVHLIYDDNTMLPVYERGEPVLGTDGKQKKAPMVQF
                     KELLGVSFVFKF"
     gene            complement(7274..8146)
                     /locus_tag="PKK03_02825"
     CDS             complement(7274..8146)
                     /locus_tag="PKK03_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HNX83357.1"
                     /translation="MKKLLGRTRDYLSLVRFSHTVFAMPFALAGYFIGATGPGYGFSL
                     KTFLLVLACMVFARSAAMGFNRWADMHFDSLNPRTAVREIPSGKITPRQALLFVIASS
                     VLFIVSAALLNRLTLILSPVALIIVLGYSYTKRFTPLCHLVLGLGLSLAPIGAYIAVT
                     GSFALLPLLYSCIVLTWVSGFDIIYSLQDDQFDRETGLYSIPAVMSRGKALAVSVVLH
                     LISLVLVVVAGIYGQAGFIYWTGSAIFIAMLIYQHMIVRPDDLSRVNLAFGTTNGIAG
                     VIFGLAVVLDLLLL"
     gene            complement(8143..10593)
                     /gene="pheT"
                     /locus_tag="PKK03_02830"
     CDS             complement(8143..10593)
                     /gene="pheT"
                     /locus_tag="PKK03_02830"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNX83358.1"
                     /translation="MKISYNWLRDYLDFDAQPDELSSVLTSLGLEVEDVEEWVSVKGG
                     MKGVVIGRVLTCERHPDADRLSVTTVDAGGTEPLHIVCGAPNVAAGQVVAVALPGTMV
                     FHGNEQYEIRKSKIRGQLSEGMICAEDELGLGADHEGIMVLDSSARIGMPAAEYFGVT
                     TDTVFEIGLTPNRIDCSSHFGVARDLAAFYNLQRPVRAKLPEIYGFRKERDRGVIKVT
                     VEAPDACIRYSGLTVRGIRVGPSPAWLQTRLRAIGLNPINNVVDITNFVLHETGQPLH
                     AFDAAAVTGDTVRVRHLPDKTKFVTLDGVERTLAPSDLMICNRDEGMCIAGVFGGLRS
                     GVTEATTDLFLESATFSPVSVRRTSRRHDLHTDASYRFERGTDPEMTIYALKRAAMLV
                     KELAGGEITGDIIDIYPEPVRKAVVRYSLDRMTKLIGKKIPDETVKTILYSLDINITG
                     EEPGILHLEIPSYRVDVTREADVTEEVLRVYGYDNIGISYEMHSMLSHTEKPDREKVA
                     SNFSEMLAANGFCEIMCNSLSPAAWFETTGDFDVKSMVRLANPLSSDLNVMRLSLLPG
                     MLNTIAWNINRQNSNLKLFEFGYTYARNEGKGTLEITDNFTEKQILALALTGNINEKR
                     WNAPEMISGFFHLKGYAEMVLSRFGIARQELVGTEENRGWFAEGMRYTAGSLDVASFG
                     KVSKKYLAMFDIRQDVFYAEIIWENLLRLISSRTIRFSELPKYPWVRRDLALLLDRSV
                     KFSQIRDLAFKTERNILREVDLFDLYESEAMGKDKKSYAVSFILHDDRQTLTEKNIEK
                     TMGALVKAFEREFGATLR"
     gene            10717..11493
                     /gene="surE"
                     /locus_tag="PKK03_02835"
     CDS             10717..11493
                     /gene="surE"
                     /locus_tag="PKK03_02835"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNX83359.1"
                     /translation="MKIEENDKLILLTNDDGLYAGGIKTLIRIMEDFGKVVVISATES
                     RSAMSQALTVKQPLRVKLLDEDDHKRIYACSGTPADGIKLAVSQLLDRKPDLIVSGIN
                     HGSNASASILYSGTMAAALEGCLYEITSVGFSLNSYSSSADFSGCEEYIRKICAMLLR
                     EPLPNGICLNVNFPALPAAQIKGMVVARQASGNWQEEFEKRKDPAGKTYYWLTGNFVN
                     HEPEASDTDDWAIQNGYVSVVPVTTDMTAYSFMETLKSKL"
     gene            11495..11791
                     /locus_tag="PKK03_02840"
     CDS             11495..11791
                     /locus_tag="PKK03_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83360.1"
                     /translation="MWFRQRFNNAISGVVAGLVMPALAFLVFFLLTHHGLSLPDYFSK
                     VEGAGNISEIMSVSVFSNIIIFLLFNRLDMLRASRGVLGITIVWAFAVFAIKLF"
     gene            11793..12926
                     /gene="lpxB"
                     /locus_tag="PKK03_02845"
     CDS             11793..12926
                     /gene="lpxB"
                     /locus_tag="PKK03_02845"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNX83361.1"
                     /translation="MRYFIIAGEPSGDLHGSNLVKHLLKTDPAADIACWGGELMRNEG
                     ARLLKHYRETAFMGVLEVLANIRKVKANFSECKRQITEMKPDVVILIDYPGFNLRMAR
                     FAKKAGLRVFYYISPKFWAWREGRVRKLKRYADRLYIIFPFEKDFYRRHDFPAIFLGN
                     PLIDHIDAWRATAPDREVIRASLGLDSRPVIALLSGSRLQEVSRILPVMAGVSAGFPD
                     YQFVVAAMDHLPARLYEEVSTAYPVRVITGRTYDLLAVAQAALVTSGTATLEAALFDV
                     PQAVCYSTSPLTYRLAKWVIKVRFISLVNLIMDREVVRELIQAGLNAVSLHRELEEIL
                     VTGYRHDRIKADYRELRTILGGGGASERVAADMSDSLVQKKGK"
     gene            12923..14074
                     /locus_tag="PKK03_02850"
     CDS             12923..14074
                     /locus_tag="PKK03_02850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNX83362.1"
                     /translation="MRRISLAAILLVLFPLVTLSQISIRIFARSKPSSLILTTVKGAC
                     ILTDGAAGEVRLSQGETVAVTRYGDRVIYSTMSGIRGASDSLTFTPAASGTLFTVRAP
                     GSSEPEKTLAGTLTVTSFPGSLRILNITTVEECLPGVVRAEAGKYGPSDYYRAQAVVA
                     RTYIYRNIDRHSLDGFNLCDDIHCQVYPGVVTDSVIVNACRSTSGLVLADRDSIMIEA
                     AFHGNCGGETASSADVWLAGYPYLVSVKDPWCSYSASSTWKRSIARSEWNGFLRSKGI
                     IPGTEGAIYSANAPAAGRITIRTVQGKSLWSEEIRLRFGLRSAYFTLSTAGDSILFSG
                     RGYGHGVGLCQDGAKHMAERKMSYNRIISFYYPGTIITDVKNARRPARP"
     gene            14109..16511
                     /gene="gldG"
                     /locus_tag="PKK03_02855"
     CDS             14109..16511
                     /gene="gldG"
                     /locus_tag="PKK03_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HNX83363.1"
                     /translation="MFTLFRKEVAGFFSSLTGYIVMIVFLLANSLIMWIMPGQWNLLD
                     SGYAGLDPLFVLSPWIFLFLVPAITMRLFSEERKSGTIELLWSRPVTEGAVIYGKFLS
                     ALFLVVMALLPALVFVISVWVLGETPGNLDRGGTAGSFIGLLMLAAVYSAIGLFASSL
                     TSNQVISFIIAAVLSFLMFRGFDSLATLPALSSVAEEVSSMGINEHYRSISRGVLDIR
                     DIAYFVFVTVLFCEAARISLRRSSIKKPLAMVAAAGAVCILLSLLHIRADLTEDRRFT
                     LAEPTKKILRELERDVKVDVYLDGELPIAFRKLRRSTEQYLDEFRITSGRKVKYDFIN
                     PSASSDRAERDRFHGELVNRGLNPVNVMASDGEGGRTQKRIFPSLTVTSGTTVIPVNF
                     LRNNPTLPAETNLLNSTEGLEYEIIQAIAAATADTVHTVAFIEGHGELDEIYVADLTL
                     ELAKFYNIDRGVIGGRRGAIDKYAAIIIAAPRKPMDERDKFVIDQYIMNGGKVLWLAE
                     EVTVNADSLIAGSTVALYEPLSTGDMLFRYGARINPVIVQDADCMLIPLKVANPGGDV
                     QYVPTPWVYYPLLRPSQTHSITRNLNRVKAEFAGTIDTVGRDPRVKKSILLTTSPEAR
                     TVMPPLVISLEEADEMIPDEMFNAGPLNVAVLLEGTFSSVFANRMAGSIMGDPQLVTV
                     TESKPTKMIMVADGDVIRNEVSWGSGNPEPLTLGLDRYTMQTFGNKDFLVNCINYLVD
                     DNGLIEMRSRELKPRLLDQTRIKEQRTLWQVVNTLLPVVLIILAGVTWNALRRRHYIS
                     LK"
     gene            16638..17768
                     /gene="dnaN"
                     /locus_tag="PKK03_02860"
     CDS             16638..17768
                     /gene="dnaN"
                     /locus_tag="PKK03_02860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNX83364.1"
                     /translation="MKFVVSSTELLGHLQAISRVISTKNTLPILDNFLFNISGNDLEI
                     TASDLESTLITRMHLDNITGSGTVALPARILLDTLKEFSEQPLTFDINPDTMAVVINS
                     ENGRFSIVGQNGIDFPVLPVIKKEKRFGFNVPSEVLLTGISRTLFATADDELRPVMGG
                     ILVEASTTNLTFVASDAHKLVRYQRSDAKADDNASFILPKKPASLLKNILPRESGVVS
                     VEFDDRNAFFNLSDYRVVCRLVEGNYPNYNSVIPKNNPRKVRIDRLEFYNILRRVSVF
                     SNQASNLVKLQFTPNQITVSAQDVDFSISAYERHKCLYEGDDMEIGFKSVFLIEILSN
                     IASQDVVIELADPTRAGLFLPSETSNEDEDLLMLLMPMMINA"
     gene            17782..18552
                     /locus_tag="PKK03_02865"
     CDS             17782..18552
                     /locus_tag="PKK03_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNX83365.1"
                     /translation="MELNLRKPLVVLDLETTGVSIASDRIVEFSALKVSPGGAEEWLT
                     LRLNPGIPISPEATRVHGITDADVANEPHFKDVAKRIAAFLEGCDLAGFNSMKFDIPI
                     LCEEFLRVNVDFNPARHRYVDVQVIFHKKEQRTLSAAYKFYCDRDLENAHSSKADTAA
                     TYEILKAQLDRYPDLENDIDRLSSFSAFNNNADLAGRIVFNEQGQEVFNFGKHKGKPV
                     EQVFREEPSYYSWMMNGDFPLNTKQVITAIKVRSFGKS"
     gene            18559..19170
                     /locus_tag="PKK03_02870"
     CDS             18559..19170
                     /locus_tag="PKK03_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNX83366.1"
                     /translation="MKIICVGLNYRKHAAEMGRPLPPEPMIFLKPDSALLKKNKPFFI
                     PDFSKNLQFEAEVVLRISKLGKGIAAKYADRYYDALTVGIDFTARDLQHKFSQAGHPW
                     ELSKTFDGSAPLGTFIPAERFPNHSDISFSLEINGEPRQEGNTSDLIFGFADIIEYVS
                     TFYTLKTGDLIFTGTPSGVGPVKKGDNLVARLAGETVLDFFVR"
     gene            complement(19163..20620)
                     /locus_tag="PKK03_02875"
     CDS             complement(19163..20620)
                     /locus_tag="PKK03_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNX83367.1"
                     /translation="MADKRLSHTYDSEEFRRYGHELIDILADYLREATSRSDMPVLDY
                     REPDDLVREFDFDNPSNRDMPLDGFVRKVVAGMNHLHHPRFVGHQNTPPLTAGIIAQM
                     CTTLLNNGVAVYEMGPASMAAERNVIRYISGLIGFGREADGIFTHGGSAGNLTGLLAA
                     RQAMSEYNVWEEGVRHDRPAGIIVSDQSHYSISRNARVIGLGGASVIRIPNDGRYAMR
                     TDLLEETWRRAEDKGIRVICVAANACSTATGSYDNLEEIAAFCREHRLWFHVDGAHGL
                     GVLFSGKYRHLVRGLEHADSVVLDFHKMLLTPGLNTMVLFRDGKRSYETFAQKAGYLF
                     EKHDSNEWYNGAKRTLECTKSALGIVAWAVINYYGKEALGKYVESRYELAARFAGAIR
                     REKGMELAVIPESNIVCFRYAPESTPAEKLNDLNRMIRKELVHSGEFYIVQTELDGNI
                     WMRTALMNALTDDRDLERLLGSIRKRGEELAQLLT"
     gene            complement(20626..22407)
                     /locus_tag="PKK03_02880"
     CDS             complement(20626..22407)
                     /locus_tag="PKK03_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNX83368.1"
                     /translation="MKRRLSAILSIMLLISGVSAQQRNATPLLKYDENKSLSWEEAIS
                     FYSDLDKKYTEATLMEMGMTDAGRPLHLFIISSDGETDPAEIRRQGKTIVLINNGIHP
                     GEPEGIDASARFAADLLANRDRMKKYLRNCSVAIIPVYNIGGALTRSRYWRINQNGPE
                     EKGARRNTRFLDLNRDFVKQDSRDAKAFAEIFQFLDPDLFLDTHTTNGSDHQFTVTLI
                     ATQPEKMHPEMEKYFREKMLPELYSRMKEEHGNEMVPYVQYTERGDIKAITGFEEEAY
                     YSTGYSALFNSFGFMTETLVYKPYHDRVIGTLQFITELVRFGSQNSKEILKLRSDANR
                     HLLAAKEYVLDWEQDTTRWDEIQYHGYSYDQTTTPITGRKSGFYNHEKPYSETIRYYN
                     YFRPAITVTVPDAYVVPFAWEEVIRRLEVNGVHMKQLQHDTTITVETYYIDGFEPARR
                     ATQGHYFNSKVNLRTVTQEVDFFRGDYLVPVNQKSKRYIITMLEPQSASGFFAWNFFD
                     SFLEGQDWYSVWGFESHLKELLDHDPALREAFEKAKQSDTNVASDPVAQLQWLYQHTP
                     ASELEKRTRLYPVARLMDAGDMFNAGN"
     gene            complement(22407..23042)
                     /locus_tag="PKK03_02885"
     CDS             complement(22407..23042)
                     /locus_tag="PKK03_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNX83369.1"
                     /translation="MAHPFIITAEEKYGQLLREKCSHIFSGTDMPSHDHLHHDRVWKN
                     AACLIERLAGAGMVTDKNLAVKAIIASFFHDTGLTVNRGPDHGIESRHICSTFLETTD
                     LAEDDRHEILDAVEKHDNKEYTGNSDPSSLAAIISVADDMDAFGEAGIGRYEEIYSMR
                     GIPAPEMPGLIVTNVLSRLRHLESTYKMFPDLIADMRVRAEKVTNHFTNRT"
     gene            complement(23106..23351)
                     /locus_tag="PKK03_02890"
     CDS             complement(23106..23351)
                     /locus_tag="PKK03_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNX83370.1"
                     /translation="MARTLTFLQLRKIKDQLPDGSIRKIADKLDLEEETVRNYFGGWN
                     FDRGQSAGIHIEKGPEGGIVTIDDTTILDLAESMLAR"
     gene            complement(23439..23873)
                     /locus_tag="PKK03_02895"
     CDS             complement(23439..23873)
                     /locus_tag="PKK03_02895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX83371.1"
                     /translation="MNVRPYKRGDFPGIMELWTVTGLSRPERGDDEATIERSIALGGE
                     MFVMTEDEDGGRITGTSWLTFDGRRLHMHHFGIIPGHQGKGLSAELLRESLRFVKEKG
                     YQVKLEVHRTNEKAVNLYKKAGFEYLGDYDVYIIRDIQSIEL"
     gene            complement(23953..25434)
                     /locus_tag="PKK03_02900"
     CDS             complement(23953..25434)
                     /locus_tag="PKK03_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HNX83372.1"
                     /translation="MKKILSVLILNLCTIAAISGSATRPVTPGNEAASDSSVLTPAGQ
                     ASQDSTTLIYRCEMKKMIAAPLWRSVKKSFAEADSLNADFMLIHMNTYGGQVDIADSM
                     RTLILNYDIPVMVFIDNQAISAGALIAVAADSIYMRSGASIGAATVVDQSGQKMPDKY
                     QSFMRGMMRATAEAHGKKPVIDGRDTTWVWHRDPEIAQAMVDPSIVLRGIVDTGQVLT
                     LTALEAIKYGFCEGQAETVEEVIENAGIKEYTIKELKLTLVDKIIMLLVNPVVSGLLI
                     MLIIGGIYFELQSPGVGFPLAAALFGALLYFAPLYLEGVAANWHVVIFVAGVILLLVE
                     IFAVPGFGVTGVLGIIGIVTGLALVMVDKIVFRFGPSVDGVKEIAGAFAIVILSAVLS
                     FVLSLWLSRKLFAPNRLFGSLALETSVNKADGFVSFDTGKLQSLVGSHGVAHTVLRPS
                     GKVMVGDEVYPAVAETGFIARGTDIIVRREEQGQLYVVAADKA"
     gene            complement(25431..26198)
                     /locus_tag="PKK03_02905"
     CDS             complement(25431..26198)
                     /locus_tag="PKK03_02905"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HNX83373.1"
                     /translation="MEKIRVSAVKYINSYPFMIGLRDGEVAGMIDLGVCHPSECADRL
                     IAGSTDLGLIPVAMIAGMKEYHLVGDYCLSTNGEVRTVLLVSNTPFEEISTIYLDYRS
                     RSSVTLCRILAAKAWKREYRWLPAPEEFDITAMSHGEAAVMIGDRCFEYARHFRYKLD
                     LGLEWKKFTGLPFVFACWVSTRRLPEQFTAIFNKALSDGLERRNEAVSLLKEGSSATP
                     EEVIFYLNHNIDFRLDDRKREALAKFIEYIKELKITP"
     gene            26323..27648
                     /gene="purB"
                     /locus_tag="PKK03_02910"
     CDS             26323..27648
                     /gene="purB"
                     /locus_tag="PKK03_02910"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNX83374.1"
                     /translation="MAISPVDGRYGERVAELVPFFSEFGLMRYRVLVEIEYFIALSRA
                     GLPQFEGFPAEKEKELLAVWNSFSLADAEFIKQKEAVTNHDVKAVEYFVKEQVSAIGL
                     EKWSEFVHFGLTSQDVNNTAMPMAIRDSVREVMKPLLVSLRDALNKMADEWADIPMLA
                     RTHGQPASPTRLGKEIAVFAERLDSELLTLMTMKYSGKFGGATGNFNAHRVAYPGVNW
                     PQFADRLLNDRLGLERQQTTTQIEHYDGLAALFDCISRINTIITDLCRDVWSYISLDY
                     FKQTIVKGEVGSSAMPHKVNPIDFENGEGNLGMANALLRHLSSKLPVSRLQRDLTDST
                     AIRNTGVAMAHTLIAWKSVKKGLSKLIVNQDRIGRDLGENWVVVAEALQTILRREGYP
                     KPYEALMALTRTNEKITGETIRLFIDTLDVTEAVREEMKAITPFNYTGF"
     gene            27658..29481
                     /locus_tag="PKK03_02915"
     CDS             27658..29481
                     /locus_tag="PKK03_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX83375.1"
                     /translation="MKSIRILLQYLRPYRWLAVQNMGFNILSAFFALFTYTLIVPFLQ
                     ILFSRVASVPDPGPFSLSASYLKDWTNWFFSSSIERYGEMRTLLIVVFVFAVASFIKN
                     LLIFLANNCMASLRAGTVRDVRNRVYDKILRLPLSYFSEARKGDLMTRVSNDVQEIEV
                     SVMSSLSMLFRDPFTIIIFVIYLFVTSPQLTLFALLMLPLSGWAIARISRSLKSASFV
                     GQQALGRLLSVLEETLTGLRIVKGFNAEDKMRRQFGVANEKFTKTFRRVARKAYLAHP
                     VSEFLSTVVLLIVIYYGGFLALRGTGNMSPDRLIAFVVVFSQIIQPAKAISSAWFSIQ
                     KGMASIDRINEVLNAGETITQKPDAVRITGFNDRIEFRNVWFAYNNDPVLKDISLTIR
                     KGQTVAVVGRSGGGKSTLADLIPRFIDPDRGAVLIDGADVRDVNIRDLRQLMGIVSQQ
                     AILFNDTFYNNISFALDEVKDEGSVERAATIANAHAFITETPDGYQNTVGEGGNKLSG
                     GQRQRISIARAIMANPPILILDEATSALDTESERLVQDAMLKLMENRTSVIIAHRLST
                     IQHADMIVVVEDGRIEECGTHEELIRNPTGIYRKLYEMQTF"
     gene            29613..30119
                     /locus_tag="PKK03_02920"
     CDS             29613..30119
                     /locus_tag="PKK03_02920"
                     /inference="COORDINATES: protein motif:HMM:NF025049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNX83376.1"
                     /translation="METKTTTDILKEAILLEQRGRAFYSSVAAQTASEAVKQIFTMIA
                     NEEEEHIRFLSEQYAHYRKHNVFGDVTVHQGDARDEVTRSVLSEEMKSQISAAGFEAA
                     AISAAMDFEAKAVELYSSRAESANDPNEKSLYRMLAEWERGHHQWLARIDKELREQVW
                     YDNNFWPF"
     gene            complement(30116..30994)
                     /locus_tag="PKK03_02925"
     CDS             complement(30116..30994)
                     /locus_tag="PKK03_02925"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNX83377.1"
                     /translation="MTTDIPLEVTGQTTLMPFLISSLKNKSRDNIKSLLRNRQVLVNG
                     EPVTQFNHELKTGDSITISATRHTGGLMARNMRLVYEDEHLVVIDKNAGLLSMASDNE
                     KYLTAYNILSTFVKSQNPSNRIFIVHRLDRDTSGLMMFARSEKVQSLMQRDWKRNVTS
                     RTYVALVEGEVTEPEGVIKSYIYESKALVMHSTRNPEKGELAVTRFRKLKSGKEYSLL
                     EVTLETGKKNQIRLHMQEIGHSIAGDKKYGATSSPIGRLGLHASVLAFIHPVTGKEMR
                     FESKIPAKFRRMVKLD"
     gene            complement(31303..31389)
                     /locus_tag="PKK03_02930"
     tRNA            complement(31303..31389)
                     /locus_tag="PKK03_02930"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31353..31355),aa:Ser,seq:tga)
     gene            31712..31984
                     /locus_tag="PKK03_02935"
     CDS             31712..31984
                     /locus_tag="PKK03_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNX83378.1"
                     /translation="MTKKELVGSIAKKAGLSLKDANIALNAFVGAFGDAMKKGQRLQL
                     PSMGTFKVEKRSARVVRNPRTGAKLNVPAKKVVKFKAAPALTKGLK"
     gene            32144..32854
                     /locus_tag="PKK03_02940"
     CDS             32144..32854
                     /locus_tag="PKK03_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNX83379.1"
                     /translation="MNCIIVDDDPVWLRTLEEFVGKSSSLSLVGSFSDSLSARNLLMS
                     RRDIELIFLDIQMPEMDGFDFLSSLENPPHIIFVSGSEEYAYKAFNYNGIDYLLKPIT
                     YPRFCRAVEKIMKYYAPRAVPSAAGEEEIFIKKASSLVRLKLKEILYVEALENYVSVI
                     TRDEKYTIHFTMRAIEQQLPSSLFVRIHRSYLVNKSVIRSISENSLTIVVGSNPKILP
                     VGKSYRDGIMNYINIMQR"
     gene            32854..34062
                     /locus_tag="PKK03_02945"
     CDS             32854..34062
                     /locus_tag="PKK03_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNX83380.1"
                     /translation="MPSQRQLFLDHIGQTSPSPLLLEIERAEGSFLYDSSGRDILDLI
                     SGVSVSNTGHRHPAVMAALNKQAGKYMHLMVYGEVIQSPQVQYAARLASLLPPSLESV
                     YFVNSGSEAIEGAIKLARRYTGRNEIIYFRNAYHGSTTGALSVQGSEEYRNAFRPLMP
                     ATTMAEFNSHEAVQMISSSTAAVLVEPVQAEAGVVQPEDSFLAGLRSACCRHGAMLIF
                     DEVQTGFGRTGSLFSLFRYNVTPDILVLAKALGGGLPLGAFIASREIISSLTRDPVLG
                     HITTFGGHPLCCATGLASLEVIVSEDLAAAAREKEKLFREELAGMPFREIRGEGLLLA
                     VVPEKPEIIPAMIAHAPEHGLLLDYFLFCSTAFRIAPPLTISEKEISLACQRLKKLVR
                     ATDSGEGLRV"
     gene            34214..36265
                     /gene="htpG"
                     /locus_tag="PKK03_02950"
     CDS             34214..36265
                     /gene="htpG"
                     /locus_tag="PKK03_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008668996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNX83381.1"
                     /translation="MEKGKIGVTTENIFPIIKKFLYSDHEIFLRELVSNATDAIQKLK
                     ALSSAGEFKGELGELRVSVTLDPKKKTLTVSDNGIGMSAEEIDKYLNQIAFSSANEFL
                     DKYKDQASGLIGQFGLGFYSAFMVSDKVEVISRSYRGNVPAVKWTCDGSPEYSIEETK
                     REEHGTDVILHIDKESKEFLEENRIEGLLKKYCSFLPVQVQFGNEKEWKDGKMVDTGK
                     PKIINNTQPAWTRKPADLKDEDYASFYRELYPMGEEPLFHIHLNVDYPFKLTGILYFP
                     KIRNNFEIQKNKIQLYCNQVFVTDSVEGIVPDFLTLLHGVIDSPDIPLNVSRSYLQSD
                     SNVKKISGHITKKVADRLTDIFKKDRETFEKKWDDLKLFIEYGMITEEKFFEKISKIT
                     LYKNTAGKYFTLEEYDKIIKENQTDKDKSLVHLYTTDPVAQHTYVAKAAEKGYDVLVL
                     DGQLDTHLINTLESKEPSSRFVRVDSDVIEKLIKKDESHASKLTQSQSDDLAQVFRTQ
                     VADTDKARFNVRAENLDENDLPMVITQSEFMRRMKDMAQFGGGYSFYGEMPDSFDLVL
                     NTNHPLVISLAGDLDKEHADELKKNGAGIEKTKVEIEFMEKEHGKKKPEEVLSVEKDD
                     LERLRGELKQLEEKRKTLLGGFGNDHQAVRQLIDLAMLANNMLKGEALSAFVKRSISL
                     L"
     gene            complement(36323..36847)
                     /locus_tag="PKK03_02955"
     CDS             complement(36323..36847)
                     /locus_tag="PKK03_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83382.1"
                     /translation="METFLEILKITIPALLVMLTAWLVIRNMLRDDQERRRQDLLLQT
                     VKTLTPVRLQAYERVVLFLERISPESLIIRTARPEMTAQQLQAALVTAVRSEYEHNLS
                     QQIYMSSEAWEMVKNARGTIVRLINSVASQLPPAATGEELSRLLLEEVVEMETEPTRT
                     AIAYIKSELARIMG"
BASE COUNT         9039 a         9712 c         9365 g         8928 t
ORIGIN      
        1 ccagagtcag ccactacgga agaatgggcg gcatgatccc gacacccgaa gaggttgttg
       61 aaaacctgaa atcattcatt aaaggataag agagatggca gaagtaacat tcaatgatat
      121 aatcaagccc gaaaacctgg cctacagaaa gtctgcactg atgacggaca atgtcatgca
      181 ctactgtccg ggctgcggac acggaactgc acaccaggtg ctggcagaag tgttggagga
      241 ggagaacctc caggaaagaa ccgtggggat caccccggtg ggctgttcag tgctgatgta
      301 caattatata gatgtcgact ttcaggaggc ggctcacgga cgcgctccgg cactggccac
      361 tgcggtcaaa cggctgatgc ctgataagtt cgtcttcacc taccagggtg acggcgacct
      421 tgctgcaatc ggcaccgcgg aaacgatcca tacctgcaac agaggtgaaa acatcctgat
      481 catcttcatc aataacggta tctatggcat gaccggaggc cagatggctc ccacaacact
      541 gccgggaatg aaatcgtcaa catcacccta cgggcgcgat gtttcaatga tgggctcacc
      601 catcaaaatg gccgatatcg tggccctgct gccgggcacc tgctatgtga cacgccagag
      661 cgtaaacact gcgggtggtg tgcgcaagtt caagaaagcg cttaaaaaag ctgtccagta
      721 tcagaatgag aaaagaggaa catgcttcat cgaggttgtg tcaaactgcc cctctggatg
      781 gaagatgaca cctgtcgagt caaacaaatg gatggaagag aacatgttcc cgttctatcc
      841 cctcggtgat attaaggtac ctgcagaata ggggccaggg ccggattgaa gcttgtttcg
      901 ctttatgccg gcaatacagt ttaagtaatc aatattacag accatgacag aagaaataat
      961 aatagcaggt tttggcggac agggagtact ctcaatgggt aaaataatgg cctattccgg
     1021 catgatgcag gatatggaag tgagctggat gccctcgtac ggccctgaga tgaggggcgg
     1081 cactgccaac gtcaacgtaa ttctcagtga tgaacgaatc agttcaccca ttctcaggag
     1141 cttcgacaca gccatcatcc tgaaccagca aagcatggac aagttcgaga agtcggtcaa
     1201 acccggggga actcttatat atgacggcaa cggtattact aggcatcccg aaaggaagga
     1261 cataaccgtg taccgcatcg acgcagccga ggaggcttca aaaatgaaga tgcagaaagt
     1321 gttcaatatg atcgtcctcg gcggattcat gaaggtgaag ccgatactga aaacagaata
     1381 tgtcctcaag ggactgaaaa aatcactccc cgaacgctac cataatctca tcccgctgaa
     1441 cgagaaggcg attgaaaggg gaatggaaat cataaataag gtctgaaggt ttgaagtctg
     1501 aaggtctgaa ggtcacaggt cttccggtct tgcaacttaa ttaattgaca ttcaggctat
     1561 tgcctactgc ctattgccta ctgcctactc ataaaagtgc atttcccgga tatatttcca
     1621 tacgggagcg ggaacaaacc acgagacatc cttcccttcg cggatggcgc tgcggataaa
     1681 ggtgcctgag atttccatga tgggtgcgtc aatctcctga actgaagcca tggccagtat
     1741 ctcctgcagc cttttgctgc ttctccggcc agggtagagc ctcggatata ccacaatagg
     1801 atactgcttt accagcatct cagcattctt ccacttatgc agtgatgtga gattatcctc
     1861 gcccatgatc agcgtgaacc gccgcgaggg atacttctct gtaagatgtg ccagtgtgtc
     1921 aatggtgtat gatggcctgg gcagggtgaa ttcaatgtcc gaagccctga accgggcatc
     1981 atcacctatt gccagctgag tcatataaaa cctgtcccgc tcgctgagaa gcgactcctt
     2041 caccttcagc ggattgtgcg gactgacaat gaaccaaacc tccttaagaa aggagtattc
     2101 ggtgatatag ttggcaatgg ccatgtgacc tatgtgcacc gggttgaatg atccgaaaaa
     2161 cagtcctgtc tcagtcatgg ccggtcaata aaatccctga caatgttcac aatggcaatg
     2221 caggctttct cgaggttgtc attgatgact accgtgtcga aatgtccggc cagcagtatc
     2281 tctgaggaag ctttctccac cctcattgca atttgttccg gtgtgtcagt cccgcgcagc
     2341 acaagcctcc gccgcagttc ctccaccgaa gggggcatga tgaagactga cagggcgtcg
     2401 tcgccgaaga tcttcttcag gttaatacct cccataacgt ctacgtcaaa gagcggtatc
     2461 ttcccttcgt cgcgtatgcg ctcaatctcc ctgcgcagcg ttccgtaata atggttttta
     2521 taaacctcct gccactcgat gaactcatca ttccggattc tcctcctgaa ttccgcagca
     2581 gtgatgaagt aatactccct tccgtctgtc tcgttgccgc gcggcttcct gctggtggcg
     2641 gaaacagaaa agaggagggg aaaccctgac tccaggagat ggcttaccag ggtcgatttt
     2701 cctgctcccg agggggcgga aatgataagc acttttttgc tgtcagccgg gcgcatccgt
     2761 ctaaaggata ttcagtgtca gctccttgat cttctccagc tcgtccttca tcataaccac
     2821 cagcttctgc atggcggcat cattggcctt tgatccgatg gtattgatct cgcggcccat
     2881 ctcctgcgag atgaagttga gcatcttccc atggttgcct tcccctttca tcgtttccag
     2941 gaagtagtcg cagtgtttac gcagcctgac cttttcctcg ttgatgtcga gtttttcaag
     3001 gtagaaaata agttcctgct caaacctgtt tctgtcgacg ttttctgatc ctacagcctc
     3061 tgccagtgat gttgtaagct tttcgcgcat tcgtgagata cggtcaccct ccacggcgcc
     3121 caggttttca agatagctga ggatggcagc aaccgacttg caaagatcct tttccatggc
     3181 cttcccctct tccaggcggt agaggtcggt catcgtcagg gcctcatcgg tgagtccgga
     3241 aattctggtc cactcctcgt cagagagctc cgccttctcc gtgcgcagtg tctcgggcag
     3301 gcgcatcact atggccaaca gcccggcatc atcactgatc cccagttcac cgctgatttc
     3361 ctttagctgg ctgaagtagc tctttacgtt gtttctgttg atcaccggca cctgctcact
     3421 ctccatggcg tcaaagctta ccgagaggtc gatcttccct ctctcgagct tcctgatgac
     3481 caggttacgg atgtccagtt ccttctcctt gcagatccag gggagcctgg tgttgatgtc
     3541 tgcctgcttg ctgttgagcg atttaatctc gacagtgatc tttctttgcg gagtctccag
     3601 catggctttg ccatagccgg tcatcgatct tatcatcgtg ttacggtaat ttcagggtta
     3661 aagataggtt tttattcgca gcggcagtga gctgacccgg tggcggagtt gtaacttaac
     3721 caagcaggtc gagctccacg gtgaagtgcc tcatgatcgg agcctcccac cttattgcca
     3781 atcctcttct gacgaggtcc cttctctccc agacgttctg caccgctgca gcaacgtagt
     3841 ccatatggtt gtcagtgtaa acccttcgcg gtattgccag cctgaccagc tcgagctccg
     3901 gatacctgtt ttcacgggtg acggggtcac ggtcggcaag cagtgttcct atctctgttc
     3961 cgcggatgcc gccctcgagg tatatctcca ccgccagcct ctgggccggg aactctgaag
     4021 caggaatgtg aggaaagaat ctgccagcat cgataaagac ggcatggcct ccgatgggca
     4081 cctgcaccgg gatcccggct tcgtgaatct tttctcccag ccttgtcacc tggcatatgc
     4141 gtgcctcgag gtaatcaaat tccgttcctt cgcgaagccc ctgtgccagt gcgttcatgt
     4201 cacggcctga catgccgccg taggtgatat agccctcgaa catgatgttg aaggtggagg
     4261 ccttcctgaa caattcgcgg tctttcatgg caatgaatcc gcccatgttg actatggcat
     4321 ccttctttga actcatggtc atggcatcag catatgagaa catctccctg actatttccc
     4381 tgatgctttt gccggaataa ccctcttcgc gtgtcttgat gaagtaagcg ttctcggcaa
     4441 agcgtgcgga gtccatcact acaagcacac catacctgtc agccagttcc ctcacaccgc
     4501 gcaggttggc catgcttacc ggctgtccgc ctgcggtgtt gttggttacg gtcatcacta
     4561 tgcatcccag cttttcacgg ggagtctcct taaggacctg ttccagtctt tcaaggtcag
     4621 catttccctt gaatggatca accaccctga tgtccctggc atatgcaaca gtacagtcca
     4681 ctgcctttgc cttgcggaat tcgatatgac ccttggtagt gtcgaagtgg gcgttaccgg
     4741 ggatcacatc tccctccttt atcagcacgg agaagagtac attttcagca gctcttccct
     4801 ggtgggtggg gaggaagtgt tcaaaaccgg tgatatcccg gattgtctct ttcagggtgt
     4861 agtaggatga ggccccggca tagctctcgt cgccggtcat gatggcaccc cactgtttgt
     4921 cgctcatcgc gccggtaccc gagtctgtca ggagatcaat gaatacctgc tctgaacgca
     4981 ggttaaacag attgtatgaa gcgtttctta tccactcctc ccgctcctgg cgggtgctct
     5041 ggcggatggt ctcaactgtc tttatcttgt atggttcaca atatggtaag atcatgggat
     5101 ttgcgatttg cgatttacga tttaagattt acgatggcct gcggccaagt cagtccgtga
     5161 ttaagaaaat ttaacaaaag caggtattca ggtgcgcaga agcgtgcaac catgatgatc
     5221 agaacgcgtc cctgttcttg atgtttcgat aattcagctt cctgtttgtg atgctgttga
     5281 agcggaaatt catggaattc ggctattttt gcaaatataa ctattttgct tttggggtac
     5341 aatcctgaac tgttaaacgt tttataatga aaccgggaac cataaagatt cttttgcttt
     5401 ttctttgcct tgccgcaacc ccggtgctga atgcacaggt tgtcgatgca ccggcgattc
     5461 cccaggtaac agcaccgcag actgatacgc tggcagtaac acaacctgca cccaggcaga
     5521 tcaaggctgc tcccgtgccg ggtatcaacg gcccgcagga tgtaaaactt gaccgggacg
     5581 agtcactcag gttccttgag cggttcagct cctcaccggc aatgtggaaa agaagcaatg
     5641 accctcttcg tgaagccatc aggaacctcc tctggcttgc ctcaaggcct ccggccgata
     5701 cggtcatcag ttacctttct gattatccat tcgacaggat aaaggtgccg gccgaaagct
     5761 attacctgtt tgactcgata aggatcatcc ttcccgtcct tcagccggac tcaacacacg
     5821 ctgacagcgt tgcagccgca cgcgctgacg agatgttcat cagaaccgga aacaaggttg
     5881 aaaaggtaag actgtcccct gattccaggc cggtcatgaa aaacgataca cttaaactga
     5941 atgactcggt ttacgtactg atgaaggagt ttatcccggc acagctgcct cacggcacca
     6001 atgacacaat cgtgttggta atcaccgata cccttccgga agtctccctg gctcgcactg
     6061 atttcccctt caggtatctc aggaatccgt atgtcagtga ctcactggaa gctgctgtca
     6121 gttcgctcat cggttatctc gaacagcgtg attccaccag gatacgcttc acaagcgacg
     6181 ggtcaagagg gaccgatgtc tggctcaata gcctttctga caacctggta cgattctggc
     6241 tgcccgatgg tcagggcgac tcagtgaccg tatggatagg caatccggtg cgcaacacac
     6301 tctcactccg tgcagaggag ggagtgctct tcagaaaaca ggcatggcat gacaggtacg
     6361 tcgacacccg cgtgaatgtc acaacagcac aggaggaggc actgaggaga gttgccctga
     6421 gcaggataaa acctgacctc tggaagttca agacagacat ctcttacctg ctctcccagg
     6481 gtgtggtctc aaactgggca aaggggggag aaaataacat atcttcagta ctcgacataa
     6541 ccacagccct gaataacaac agcaagcttt caaaggtgat ttcagccagc acgggtcgat
     6601 ttgcacttgg actccaggct tccggcaaat atggcggcat aaggaaaaac ctcgatatcc
     6661 tcgagattaa ctccaagatc aatcacaagg ccttcggcaa attcgacctc tcggggatct
     6721 ttcagttcaa gtcgcagttc ctgcccggtt acacataccc gaatgacacc acctcattat
     6781 tggtctccag gttcttcaat cctgccacat tcatcctggg ttacggtctt gaatacaaac
     6841 ccgacaaaaa cacctcggtc agtttctctc cattttcata taaaggcaca tttgtccctg
     6901 acaccgcaaa catcaaccag accaaatacg gtattgctgc cgacaggagg tcaaagaacg
     6961 agctcggcgc atacctgacc atcaacagca agcttgtgct cttcgagaag gtaaacatga
     7021 ccaacaggtt acagctcttc agcaacttcc tctccaagcc ccagaatata gacgttgagt
     7081 gggagatgat atccacgatg ccgcttaact ggttcactga cctcagggtg aatgttcacc
     7141 tgatttatga tgacaacacc atgcttccgg tttatgaaag gggagagccg gtgcttggca
     7201 ccgatggcaa gcagaagaag gcgcccatgg tgcaattcaa ggaactgctt ggcgtcagct
     7261 tcgtctttaa gttttaaagg aggagcaggt caagcaccac cgccagtccg aaaatgacac
     7321 cggcgattcc gttggtggtc ccgaaggcca agtttaccct gctcaggtca tcaggcctga
     7381 caatcatgtg ctgataaatg agcatggcaa tgaatatggc tgaaccggtc cagtaaatga
     7441 accctgcctg gccgtaaatt cctgcaacca ccaccagtac caacgagatc aggtgaagca
     7501 caactgaaac agccagcgct ttgccgcgcg acatcaccgc cgggattgaa tagagtccgg
     7561 tttcacggtc aaactggtca tcctgaaggg aatagattat atcaaaaccg ctcacccatg
     7621 tcagtacgat gcaggagtac aggagaggaa gcagggcaaa agagcctgtc actgctatgt
     7681 atgctccgat gggtgcaagg gagagtccca gccccaggac caggtgacac agcggggtga
     7741 accgcttggt ataactgtac ccgagtacaa ttatcagggc caccggagaa agtatgaggg
     7801 tcagcctgtt gagcagggca gcggagacaa tgaataagac tgaactggct attacaaaca
     7861 aaagtgcctg gcggggagtg atcttccctg acgggatctc acgaactgct gtacgcggat
     7921 taagtgagtc aaaatgcatg tcagcccacc ggttgaaacc cattgctgca ctccgtgcga
     7981 agaccatgca tgccagaaca agaaggaaag tcttcagtga gaagccgtat cccggcccgg
     8041 ttgccccgat gaagtaaccg gcaagggcaa agggcatcgc aaagacagtg tgactgaacc
     8101 tgacgagcga aagataatcc cgtgttctgc caagcagctt tttcatctca gcgtggcacc
     8161 gaactcacgc tcaaaggctt tgaccagggc gcccatggtc ttttctatat tcttttcggt
     8221 aagtgtctgt ctatcatcat gaagtatgaa gctgacggcg tacgacttct tgtcctttcc
     8281 catagcttcg ctctcataga gatcaaagag gtctacctcc ctgagtatat ttctttcagt
     8341 tttgaaagca aggtcacgta tctggctgaa tttcactgac ctgtcaagaa gaagggccag
     8401 gtcacgccgc acccacgggt atttgggcaa ttcgctgaaa cggatggtcc ggctgctgat
     8461 aagccttaaa aggttctccc agatgatctc ggcgtagaat acatcctgcc tgatatcaaa
     8521 cattgccagg tatttttttg acaccttgcc gaaggaggcc acgtcaagac ttccggcagt
     8581 atatctcatc ccttcggcga accatccccg gttttcctcg gttccaacga gctcctgcct
     8641 ggcgatgccg aaccgtgaaa ggaccatctc agcatagccc ttcaggtgaa agaatcctga
     8701 gatcatttca ggggcattcc accgtttttc gtttatattc ccggtcagcg ccagggcaag
     8761 tatctgtttt tcagtgaagt tgtcagtgat ctccaaggtg cctttgcctt cgttccgggc
     8821 gtaggtgtat ccgaactcaa aaagtttcag gttgctgttt tgcctgttga tgttccatgc
     8881 aatggtgttc agcatacccg gcagcagcga gagacgcatg acgttcaggt cactgctgag
     8941 cggattggcc agcctgacca ttgacttcac gtcgaagtcg cccgtagtct caaaccaggc
     9001 agcaggggaa agagagttac acattatctc gcagaagccg ttggcagcaa gcatctctga
     9061 aaaattggag gcaactttct cgcggtcagg tttttcagta tgtgacagca tggagtgcat
     9121 ctcatagctg atccctatgt tgtcataacc gtaaaccctg agcacctctt cagtgacatc
     9181 ggcttcgcgg gtgacatcaa cacggtacga tggaatctca agatgcagaa tgccgggttc
     9241 ttcaccggtg atgttgatat cgaggctgta gagtatggtc ttgaccgttt catccggtat
     9301 tttcttccct atgagttttg tcatccggtc aagcgaatac ctcacaactg cttttctcac
     9361 cggttcgggg tagatatcaa tgatatcgcc tgttatttca ccgccggcaa gctccttaac
     9421 cagcatggca gctctcttca gagcgtagat tgtcatctcc ggatcagtgc ctctctcaaa
     9481 cctgtatgat gcgtcagtgt gcaggtcatg cctccgcgat gtgcgacgta cggacaccgg
     9541 gctgaaggtg gcgctctcaa ggaaaaggtc ggtagtggct tctgtgactc ctgatctcag
     9601 tccgccgaag acacccgcta tgcacatccc ctcgtcacgg ttgcatatca tcaggtcgga
     9661 cggtgcaagg gtgcgttcca ctccgtcaag ggtaacgaat ttcgtcttgt caggaaggtg
     9721 ccttaccctc actgtgtcgc cggtaacggc tgcagcatca aatgcatgca gtggctgtcc
     9781 tgtctcgtgg aggacgaagt tagtgatgtc aacaacgttg ttgatgggat tgagcccgat
     9841 tgccctgagc ctggtctgta accacgccgg cgaaggacca accctgatgc cgcgcaccgt
     9901 caggccggag taacgtatgc atgcatccgg agcctcaacg gtgactttta taacacccct
     9961 gtccctttcc ttcctgaacc cgtatatctc gggcagcttt gcccttaccg gtctctggag
    10021 gttatagaag gcagcgagat cgcgtgccac accaaagtgt gagctgcagt caatcctgtt
    10081 cggtgtcagc cctatctcaa agacggtgtc ggttgtaact ccgaaatatt ctgcagcagg
    10141 catgccaata cgtgcgctgc tgtcaagcac cattattcct tcgtggtcag cgcccagtcc
    10201 aagctcgtcc tcggcacaga tcatcccctc ggacagctgc ccgcgtatct ttgatttcct
    10261 gatctcatac tgttcattac cgtggaagac cattgttcca ggcagggcca ccgccaccac
    10321 ctgtcctgca gctacgttcg gtgccccgca tacaatatgc agcggctccg ttcccccggc
    10381 atctactgta gtgacactca gcctgtcagc atcagggtgc ctttcacagg tgagtaccct
    10441 gccgataacc acacccttca ttccaccctt taccgaaacc cactcttcaa catcctcaac
    10501 ctcgagaccc agtgatgtca gaaccgatga cagctcatcg ggctgcgcat caaaatcaag
    10561 gtaatccctg agccagttgt aagaaatctt catcgcggta ttgttaattg gtttttaaaa
    10621 atgcaaaagt aaggaaaaaa ggtttgaggc atcaagcagg cgcggtatta aaagcagagt
    10681 cattatcatt atatttgcac gtcaacagaa atccagatga aaatagaaga gaacgacaag
    10741 ctgattcttt taacaaatga tgacggactc tatgcaggcg gaataaaaac cctcatcagg
    10801 atcatggaag acttcggcaa ggtggttgta atctcagcca ccgagagccg cagcgccatg
    10861 tcacaggccc tgacagtcaa gcagcccctg agggtcaagc tcctcgatga ggatgatcac
    10921 aagcggatct acgcctgcag cggcacaccg gccgatggta tcaaactggc ggtcagccag
    10981 ttgctcgacc gcaagcctga cctgatagtc tcaggcatca accatggctc caacgcctcc
    11041 gccagcatac tctattcagg tacaatggcc gctgctcttg aaggatgcct gtatgaaatc
    11101 acgtcggtgg gattctcact taacagctat tcgtcctcag ccgacttttc agggtgcgaa
    11161 gagtacatca ggaaaatttg tgcgatgctc cttcgcgagc ctttgccgaa cggtatatgc
    11221 ctcaatgtca acttcccggc actgccggca gctcagatta aagggatggt ggtggcgcgc
    11281 caggcctcag gcaactggca ggaggaattt gagaagagaa aagatcctgc gggaaagacg
    11341 tactactggc tgacaggcaa ttttgtcaac catgagccgg aggcttcaga tactgacgac
    11401 tgggcaatcc agaacggcta tgtctctgtt gtgccggtga ctactgacat gactgcctac
    11461 agcttcatgg aaacccttaa atcaaaactc tgacatgtgg ttcaggcaaa ggttcaataa
    11521 tgccatatct ggtgtggttg caggattggt gatgccagcc ctggctttcc tggtattctt
    11581 tctgctgaca catcacgggc tttcgctgcc tgattatttc agcaaggtgg aaggtgccgg
    11641 aaatatttcg gaaataatga gtgtctccgt cttctccaat ataatcatct ttctgctctt
    11701 taaccgcctt gacatgctga gagcctccag gggtgtgctt ggaataacca tagtgtgggc
    11761 ctttgctgtc ttcgcgataa agctgtttta ggatgaggta cttcatcatt gcaggcgagc
    11821 catccggtga tcttcacggt tccaacctcg tgaagcacct gctaaagact gatccggcag
    11881 ctgatattgc atgctgggga ggtgaactga tgcggaacga aggcgcacgg ctgctgaaac
    11941 actaccgtga aactgccttc atgggcgttc tcgaggtgct tgccaacatc cggaaggtga
    12001 aagccaactt cagtgaatgc aaaagacaga taacggaaat gaaacccgat gtggtgatcc
    12061 ttattgacta ccctggtttc aatctgcgaa tggccaggtt tgcaaagaag gctggcctga
    12121 gggtcttcta ctacatttcg ccgaagttct gggcatggcg cgaaggaaga gtcagaaagc
    12181 taaaacgcta tgcagacagg ctttacatca tctttccgtt tgaaaaggat ttctaccggc
    12241 gacatgattt cccggcaatc ttccttggca atcccctgat tgaccatata gatgcatgga
    12301 gggccacggc ccctgacagg gaggtaatca gagcctcact gggacttgac agcagacctg
    12361 taatagcact tctctccggc agcaggcttc aggaggtaag caggatcctt cctgtcatgg
    12421 ccggagtgtc tgccggcttt cctgattacc agttcgtggt ggccgcgatg gatcatctgc
    12481 cagcccggct ttacgaagag gtctctacag cataccctgt cagggttatc accggcagaa
    12541 cctatgacct cctggctgtg gcgcaggccg cgcttgtcac ctccggcact gcaacccttg
    12601 aggctgccct gtttgatgta ccccaggcag tatgctacag taccagtccg cttacataca
    12661 ggctggcaaa atgggttatt aaggtccggt tcatctcgct ggtgaacctt atcatggaca
    12721 gggaggtggt gagagagctg attcaggccg gactgaatgc tgtcagcctg cacagggaac
    12781 tggaggagat actcgtcacc ggttacaggc atgacaggat aaaggccgat taccgtgaac
    12841 tgcggaccat actcggaggt ggcggggcat cagagagggt agcggcggat atgtcagaca
    12901 gtcttgttca aaagaaaggg aaatgaggag aatctcactg gccgccatac tgctcgttct
    12961 gtttccactg gttacacttt cccagatctc aatacgtatc ttcgcccggt caaagccttc
    13021 cagcctgatt ttaaccacgg tcaaaggtgc ctgtatcctt accgacggag ctgcgggaga
    13081 agtacgtctc agccagggtg aaaccgttgc cgtgacacgg tacggtgaca gggtaattta
    13141 cagcacaatg tcaggcataa gaggagcatc tgactcactc acctttaccc cggccgcatc
    13201 cggcactctc ttcacggtcc gggcacccgg cagcagcgag cctgagaaga ccctggccgg
    13261 cacactgaca gtcacttcat tcccaggttc actccggata ctgaatatca caaccgttga
    13321 agaatgcctt ccgggagtgg tccgggccga ggcgggcaaa tacggaccat cagattacta
    13381 cagggcacag gccgtggtag ctcgaacata tatctacagg aatatcgaca ggcattctct
    13441 cgacgggttt aacctgtgcg atgatatcca ttgccaggtc tacccgggag tggtgaccga
    13501 cagcgtgatt gtgaacgcct gccgctcaac gtccggactg gtactggcag accgcgacag
    13561 cataatgata gaagccgcgt tccacggcaa ctgcggcggt gagacggcat cttcagccga
    13621 cgtgtggctt gccggttacc cgtatctcgt gagcgtgaag gatccctggt gcagctattc
    13681 cgcatcatcc acatggaaga ggagcatcgc gcgcagcgaa tggaacggtt tcctgagatc
    13741 gaagggcata attccgggaa cggagggagc catctattcg gcgaacgcac cggcggcagg
    13801 aaggataaca atccgtactg tacagggaaa gtcattgtgg agcgaggaga tccggctccg
    13861 cttcgggctc aggtcagcct actttactct ttcaaccgcc ggtgacagca ttcttttcag
    13921 cgggagagga tatggccatg gtgtggggct gtgccaggac ggagcaaagc atatggctga
    13981 aagaaagatg agttataaca ggataatcag cttctattac ccgggcacca taattactga
    14041 tgtcaaaaat gcccgcaggc cggccagacc atgaaagttt tagatatttt tacagcaaac
    14101 ttgaagttat gttcactctt ttcagaaaag aggtagcagg ttttttcagc tcccttacgg
    14161 gttatatcgt aatgattgtg ttcctcctgg ccaacagcct gatcatgtgg atcatgccgg
    14221 gccagtggaa cctgctggac agcggctatg ccggccttga ccccctcttt gtgctctcac
    14281 cgtggatctt ccttttcctg gtgcccgcaa ttaccatgag gctcttttcg gaagagagaa
    14341 aatccggaac cattgagctg ctctggtcgc gcccggtgac tgaaggcgcc gtgatctatg
    14401 gcaagttcct ttcagcgctg ttcctggtgg taatggcctt attgcctgcc ttggtattcg
    14461 tcatctcggt gtgggtgctg ggtgagaccc ccggtaacct tgacagggga ggtaccgcag
    14521 gatctttcat cggactcctg atgcttgccg cagtctactc cgccatcggc ctctttgcct
    14581 catcactcac ctcgaaccag gtgatctcat ttattattgc agccgttctc tccttcctga
    14641 tgttcagggg atttgactcg cttgcaacac tgcccgccct ctcatccgtt gcggaggagg
    14701 tctcctccat gggcatcaat gaacactacc gctcaataag caggggggtg ctcgatatcc
    14761 gtgacatagc ctacttcgta tttgtcacgg tgttgttctg cgaggctgcc agaatatcac
    14821 tccgccgcag cagcatcaaa aaaccgcttg ccatggtggc agcagccgga gcggtctgca
    14881 tacttttatc gttactgcac ataagggctg acctgaccga agaccggcgc ttcacactgg
    14941 cagaaccgac caaaaagata ctcagggaac tcgaacgcga tgtgaaggtg gatgtttacc
    15001 tcgacggcga actgccgata gccttccgga aactgcggcg cagcactgaa cagtacctcg
    15061 atgagttccg gataacctca gggaggaagg tgaagtatga ctttatcaat ccctcagcat
    15121 cttcagacag ggcagagcgc gaccggttcc acggtgaact cgtaaaccgc ggcctcaacc
    15181 ccgtaaatgt tatggcctca gatggagagg gaggcagaac ccagaaacgg atcttcccct
    15241 cacttactgt caccagcggc actacggtca tacccgtgaa ctttctgagg aacaatccca
    15301 ccctgcctgc tgagacaaac ctcctgaatt ctactgaagg actcgaatat gagatcattc
    15361 aggcaattgc tgcagcaacg gctgatacag tgcacacagt ggccttcatt gaaggtcacg
    15421 gtgaactcga tgagatctac gtggctgatc tgaccctgga actggccaag ttctacaata
    15481 ttgacagggg ggtgataggg ggcaggaggg gagccattga taaatatgcc gccattatca
    15541 tagccgcacc acggaaaccg atggatgaaa gggataagtt tgtgattgac cagtatatca
    15601 tgaacggcgg aaaggtactc tggcttgcag aagaggtgac ggtgaatgct gacagcctga
    15661 ttgcaggatc tacagtggcg ctgtacgaac ccctctcgac aggcgacatg cttttcaggt
    15721 acggtgcccg catcaacccg gtgattgtgc aggatgccga ctgcatgctg atccctctga
    15781 aggttgccaa tcccggaggc gatgttcagt atgtgcccac accatgggtc tattatccgc
    15841 tgctgcgtcc ttcacagacc cactccatca cacgtaacct caacagggtc aaggcagagt
    15901 ttgccggtac cattgacacg gtgggacggg atccgcgggt gaaaaagagc atccttctca
    15961 caacctcgcc tgaagcccgt acagtaatgc ccccgctggt catctcactg gaggaggctg
    16021 acgaaatgat ccctgacgag atgttcaacg ccggccctct gaatgtggca gtgctcctcg
    16081 aaggaacctt cagctctgtc ttcgcaaacc gcatggcagg cagcattatg ggtgaccctc
    16141 aactggtgac cgtgaccgaa agcaagccca caaagatgat tatggttgct gacggggatg
    16201 taatacgcaa cgaggtctcc tggggaagcg gcaatcccga accgctgacc ctcggccttg
    16261 acaggtacac catgcagacc ttcggcaaca aggacttcct tgtgaactgc atcaattacc
    16321 tcgttgatga caacggactc atcgaaatgc gttcacggga actgaagccc agactgctag
    16381 accagacccg cataaaggag cagagaacac tctggcaggt agtgaacaca cttctgcctg
    16441 tagtgcttat aatcctcgca ggagtgacct ggaacgcact cagacgcagg cattatatat
    16501 cattgaaata aaatgtttga gggtaatata tttttttgta atattgataa tccggtttta
    16561 aaagcagacc ttattagtaa tcaattggtt gccggtaaac tgagattcat tgccgaataa
    16621 attaaacaga ccgaaatatg aaatttgttg tttcaagcac agaactcctg ggtcacctgc
    16681 aagccatcag cagggtgatc agtacaaaga atacccttcc gattcttgat aattttcttt
    16741 ttaacataag cggaaatgat ctggagataa cggcttcaga cctcgaatcg acgcttatca
    16801 cgcgcatgca tcttgacaat atcacaggaa gcggaacggt agcccttccc gccaggatac
    16861 tgcttgacac cctcaaggag ttctcggaac agccgctcac ctttgatatc aatcccgaca
    16921 ccatggccgt cgtcataaat tcagagaacg gccggttcag cattgttggt cagaacggga
    16981 ttgactttcc cgtgctgccg gtcatcaaga aggagaaacg ttttggcttc aacgtacctt
    17041 cagaggtgct ccttaccggt atctcaagga cactgtttgc aacagctgac gatgaacttc
    17101 gccccgtaat gggaggcata cttgtggagg cttcaaccac caacctgaca ttcgttgcat
    17161 ctgatgcaca taagctggtc aggtaccaga gaagtgacgc caaagctgac gataacgcat
    17221 cattcatcct gccaaagaag cctgcttcac ttctgaagaa tatactccca agggagagcg
    17281 gcgtggtatc ggtagagttc gatgacagaa acgcattctt caatctctct gactacaggg
    17341 ttgtgtgccg cctggttgaa ggcaattatc ccaattacaa ctccgtcatc ccgaagaata
    17401 atcccaggaa agtccgcatt gacaggctcg agttttacaa catcctccga agggtctccg
    17461 tattctcgaa ccaggcaagc aacctggtca aactgcagtt cacgcctaac cagataaccg
    17521 tgtcagcaca ggatgtcgat ttctccatct cggcatacga gcgccacaag tgcctctatg
    17581 agggtgatga catggagatt ggcttcaagt cggtcttcct gattgaaata ctatcaaaca
    17641 tcgcatcaca ggacgtggtt attgagcttg ctgaccccac cagggcagga ctgttcctcc
    17701 cctccgagac gtccaacgag gatgaggatc tcctcatgct tctgatgcca atgatgataa
    17761 acgcctgaga atcagcacga catggagctt aacctcagaa aacccctggt cgtccttgat
    17821 cttgagacca cgggggtgag tatcgcatct gatcgtatcg tggaattttc tgcccttaag
    17881 gtttcgccgg gaggcgctga agaatggctt accctgagac tgaacccggg cataccaata
    17941 tcaccggagg caacacgggt tcacggtatc actgacgccg atgtggccaa tgagcctcac
    18001 ttcaaggatg tggcaaaaag aattgccgct ttcctcgaag ggtgtgacct tgccggattc
    18061 aactcaatga agttcgacat acccatactc tgcgaagagt ttctcagggt aaatgttgat
    18121 ttcaaccctg cccggcacag gtacgtagac gtgcaggtga tatttcacaa gaaggagcag
    18181 aggaccctca gcgctgctta taaattctac tgtgacaggg atttggagaa tgcccacagc
    18241 tcaaaggctg acactgctgc cacttatgag atcctgaagg cgcagcttga caggtatcct
    18301 gacctggaga atgatattga caggctttct tcattcagtg cattcaataa caacgctgat
    18361 ctggcaggaa gaatagtctt caatgaacag ggacaggagg tcttcaactt cggcaagcac
    18421 aagggcaagc ctgttgaaca ggtattcagg gaggaacctt catactactc ctggatgatg
    18481 aatggtgatt ttcccctcaa caccaagcag gtgattaccg ccatcaaggt gcgttcattt
    18541 ggcaaatcgt agggtttcat gaaaataatc tgtgtcggac ttaactaccg caagcatgcg
    18601 gcagagatgg gtcgtccgct ccccccggag ccaatgatct tcctgaaacc cgattcggcc
    18661 ctgctgaaaa aaaacaagcc cttctttatc cctgacttct caaagaacct ccagtttgaa
    18721 gcagaggtgg tactcaggat ttcgaagctt ggtaaaggta tcgctgccaa atatgctgac
    18781 cgctattatg atgcactgac tgtcgggata gacttcactg cgcgtgatct gcagcataag
    18841 ttctcacagg ccggacaccc atgggagctc tcaaagacgt ttgacggttc ggcacctctt
    18901 ggcactttca tcccggcaga gcggtttcca aatcattcag atatcagttt ctctctggaa
    18961 ataaacgggg agccaagaca ggagggaaat acatctgacc tgatattcgg ttttgcagac
    19021 ataatagaat atgtctcgac gttttatact ctcaagacgg gtgacctgat ttttacgggc
    19081 acaccgtcag gggttggccc ggtcaaaaag ggagacaatc ttgtggcgcg cctggccggt
    19141 gagacagtac ttgacttttt tgtcaggtaa gcagctgagc cagctcttcg ccccgtttcc
    19201 ttatactgcc gagaagtctc tcgaggtccc tgtcatccgt cagggcattc ataagcgccg
    19261 tacgcatcca gatgttgccg tcgagctctg tctgcacgat atagaactcg ccgctgtgca
    19321 caagctcttt cctgatcatc cggttaagat cgtttaattt ttccgcgggg gtgctctccg
    19381 gtgcatacct gaagcaaact atatttgatt ccgggattac agccagttcc attcctttct
    19441 ccctgcgtat agccccggca aaccttgccg caagctcata cctgctttca acatatttcc
    19501 ccagggcttc tttcccgtaa taatttatga ccgcccaggc cactatgcca agggcgcttt
    19561 tggtgcattc cagggtcctt tttgcgccat tgtaccactc attcgaatca tgtttctcga
    19621 acaggtagcc cgccttctgc gcaaaggtct catatgacct tttgccgtca cggaagagaa
    19681 ccatggtgtt caggcccgga gtgagcagca tcttgtgaaa atcaagcaca acagagtcgg
    19741 catgctcaag ccccctgacc agatgacggt atttaccgga aaaaagtact ccgagaccgt
    19801 gcgccccgtc tacatggaac cagagcctgt gctcacggca gaaagcagct atctcctcca
    19861 ggttgtcata ggagcctgtg gcagtggagc aggcgttggc agctacacag atcaccctga
    19921 tgcccttatc ttcagccctc cgccaggtct cctccaggag atcggttctc atggcatagc
    19981 ggccgtcgtt ggggatacgt atcactgacg ccccgccaag gccaatcacc ctggcattgc
    20041 gggagatgct gtaatgcgac tggtcagaga caatgatccc ggccggcctg tcatgcctga
    20101 caccctcttc ccatacattg tattcactca tcgcctggcg tgccgcgaga agccctgtca
    20161 ggttacccgc acttccaccg tgggtgaaga taccgtcggc ctcacggccg aagccgataa
    20221 gcccggagat atatctgatg acgttgcgct cggcagccat cgaggccgga cccatctcat
    20281 ataccgcgac cccgttgttg agcagtgtgg tacacatctg agcaatgata cctgccgtaa
    20341 ggggaggggt gttctgatgc cccacaaaac ggggatgatg aaggtgattc atgccggcaa
    20401 caaccttccg gacaaaaccg tcaagcggca tgtcacggtt tgacgggttg tcaaaatcaa
    20461 actccctgac gaggtcatcc ggttcacggt aatcgaggac tggcatgtca ctccgggagg
    20521 ttgcttcgcg gagataatca gccagtatgt ctatcagttc atgcccgtac ctgcgaaact
    20581 cctcgctgtc ataggtgtga ctgagtcttt tgtctgccat aaatatcagt tgcccgcatt
    20641 aaacatgtcg cctgcatcca tgagcctggc caccgggtac agcctcgtcc tcttctccag
    20701 ttctgaagca ggggtatgct ggtaaagcca ctgcaactgg gccacggggt cggatgcaac
    20761 attcgtgtct gactgcttgg ccttttcaaa agcctcccgc agggcgggat catggtcaag
    20821 caactccttc aggtgactct caaaacccca taccgagtac cagtcctgcc cctcgaggaa
    20881 cgaatcgaaa aagttccatg caaagaagcc tgatgcgctc tgcggctcaa gcatggtgat
    20941 gatgtatctt ttcgatttct ggtttacagg caccagatag tcgcccctga agaaatccac
    21001 ttcctgggtg acagtgcgca aattcacctt gctgttgaaa tagtgtccct gggtggctct
    21061 cctggcaggc tcgaacccgt caatgtaata ggtttcaacc gttatggtgg tgtcatgctg
    21121 cagctgtttc atatgtacac cgttaacctc caggcgcctg ataacctctt cccatgcgaa
    21181 tggcactacg taggcatcgg gcacggtgac tgtgatggcc ggcctgaagt agttgtagta
    21241 cctgatggtt tcagaatagg gcttttcatg gttgtagaaa cctgattttc tgccggtgat
    21301 gggggtggtt gtctggtcgt aactgtaacc atggtactga atctcatccc acctggtggt
    21361 atcctgttcc cagtccagaa cgtactcctt agctgccaac agatgccggt tggcgtcaga
    21421 tcgcagctta aggatctcct tgctgttctg tgacccgaac cttaccagct cggtgatgaa
    21481 ctggagcgtc ccgatgactc tgtcatggta tggcttgtag accagtgtct cagtcatgaa
    21541 tccgaatgaa ttgaagaggg cggagtaacc ggttgagtag taagcctcct cctcgaagcc
    21601 tgtgatggcc ttgatatctc ctctctcagt atactggacg taaggcacca tctcattccc
    21661 gtgctcctcc ttcatacggc tgtacagctc cggcagcatt ttctcacgga agtacttctc
    21721 catctccggg tgcatttttt caggctgtgt ggcaatgagc gttacagtga actgatggtc
    21781 agatccgttc gtggtatgcg tgtcgaggaa gagatccgga tcaaggaact ggaatatttc
    21841 tgcaaaagcc ttcgcgtcac gcgagtcctg cttcacgaag tcgcggttca ggtccagaaa
    21901 ccgtgtgttc cgccgggctc ccttttcctc agggccgttc tggttgatcc gccagtaacg
    21961 gcttcgcgtc agcgctcctc ctatattata aacaggtatg atggccaccg aacagttccg
    22021 aaggtacttc ttcatcctgt ccctgtttgc aagcaggtca gctgcaaacc tggcgctggc
    22081 gtcaatgcct tccggctcac cgggatgtat tccgttgttg atgagcacaa tggttttacc
    22141 ctgcctgcgt atctcggcag gatcggtctc cccgtcggag gaaattatga ataaatgaag
    22201 cgggcggcct gcatctgtca tacccatctc catgagggta gcctcagtgt attttttatc
    22261 aaggtcactg taaaaactga tggcctcttc ccatgaaagg ctcttgttct catcatactt
    22321 cagaagaggt gttgcattgc gttgctgcgc cgatacgccg gatatgagca gcattattga
    22381 caggattgct gaaagtcttc ttttcatcag gttctgtttg taaaatggtt ggttactttt
    22441 tcagcccgta ccctcatgtc tgcaatgagg tcagggaaca ttttataggt tgactcaaga
    22501 tgcctcagac gtgaaagcac gtttgtgact atcagcccgg gcatctccgg ggcgggaata
    22561 cccctcatgg agtatatctc ctcgtaccgg cctatgcctg cctctccgaa ggcatccatg
    22621 tcatctgcca ctgaaattat ggctgccagt gaggagggat cagagttgcc ggtgtactcc
    22681 ttgttgtcat gcttctcaac tgcgtcaagt atttcatgac ggtcatcttc tgcaaggtca
    22741 gtcgtttcaa gaaatgtgct gcatatatgc ctgctctcaa tcccatggtc ggggccacgg
    22801 tttactgtca gcccggtatc atgaaagaat gaagctatta tggctttgac tgccaggttc
    22861 ttatcagtga ccatgccggc tccggcgagc cgctcgataa gacaggctgc atttttccac
    22921 acccggtcat gatgcaggtg atcatgcgat ggcatgtcag tgccagagaa aatatgactg
    22981 cacttctccc ggagaagctg tccgtacttc tcctcagcag ttatgatgaa cggatgcgcc
    23041 atgactgaaa acaaaaaggc aaaagtagga acttttgcct ttctggttcc ctgtaaatat
    23101 cagaattagc gggctagcat gctttcggca aggtcaagta tggtagtgtc atctattgtg
    23161 actatgcccc cttcgggtcc tttttctata tggatcccgg cactctgccc gcggtcaaag
    23221 ttccagccac cgaaataatt cctgacggtc tcctcctcca aatcaagctt gtcagctatc
    23281 tttcttatgc tcccgtcagg aagctggtcc ttgatcttcc tcaattgtaa aaatgttagt
    23341 gttcgtgcca tgatttaaaa cggatagatt tcttcaaata tactaaatat tcattgaaag
    23401 gggaaatata aaaggctctt taaggttatt ttaacatttt ataattcaat ggattgaata
    23461 tcacggatta tgtacacatc ataatcgcca aggtactcaa aacctgcctt tttgtataga
    23521 tttacagctt tttcatttgt tctgtgaacc tccagcttta cctggtatcc cttttccttt
    23581 acaaacctga gtgattctct cagcagttct gccgacaatc cttttccctg gtgcccgggg
    23641 attatgccga aatggtgcat atggagccgc ctgccgtcga aggtaagcca ggaagtgccg
    23701 gttatcctcc cgccgtcttc atcttccgtc atcacaaaca tttcgcctcc caaggcaatg
    23761 ctccgttcaa tggttgcctc atcatcccct ctttccggcc tgctgagccc tgtcactgtc
    23821 catagctcca ttattcccgg aaaatcacct cttttgtatg gtcttacatt catcctgcca
    23881 tcaacttttt gcaatcagtg taatctccat tgctgtcatg cggcagaccg gtctcaccgg
    23941 tcacctcccg gctcaggctt tatctgcagc taccacgtaa agctgaccct gttcttccct
    24001 gcgcactata atgtctgttc cccttgctat gaagcctgtc tctgccactg ccgggtaaac
    24061 ctcgtcgcct accatcacct tccctgacgg gcgaagcacg gtatgagcca caccatggct
    24121 tccgacgagc gattgaagtt tgccggtgtc aaagcttaca aatccatcgg ccttgttcac
    24181 cgatgtctca agagccagac tgccgaagag cctgttcggg gcaaagagct ttctcgaaag
    24241 ccataatgaa agcacgaatg aaagaacggc tgacagaata actattgcga aagctcctgc
    24301 tatctctttt acaccgtcaa ccgaaggccc gaacctgaag actatcttgt caaccattac
    24361 aagggcaaga ccagtcacaa tgcctatgat tccgaggacc cccgtcactc cgaaacccgg
    24421 aacggcaaat atctccacca gtagaagtat tactcctgca acgaatatca cgacatgcca
    24481 gttggcggct acaccttcaa ggtaaagcgg ggcgaagtag agcagggcac cgaagagggc
    24541 agctgccagg gggaagccca ctcctggcga ctggagctca aagtagatac ccccgatgat
    24601 cagcattatc agcagccctg agaccacggg atttaccagc agcatgataa tcttgtcaac
    24661 cagcgtaagc ttcagctcct ttatggtata ttcctttatc ccggcgtttt ctattacttc
    24721 ctcgaccgtc tcggcctgcc cttcgcaaaa gccgtatttg attgcctcca gcgcggtcag
    24781 tgtcagtacc tgtcctgtgt caacaatccc cctgaggaca atcgaggggt ctaccatggc
    24841 ctgcgctatc tccggatcac ggtgccatac ccaggtggtg tcacggccgt caataaccgg
    24901 cttcttgccg tgggcctcag cagtggctct catcatcccc ctcatgaacg attggtactt
    24961 gtcaggcatc ttctgcccgc tctggtctac cactgtggct gccccgatgc tggctcccga
    25021 gcgcatataa atactgtcag cagcaacggc tatcagcgca cctgcggaaa tagcctggtt
    25081 gtcaatgaag accatcacag ggatatcata gttgagtatg agggttcgca ttgagtcggc
    25141 aatatcaacc tggccgccat aggtattcat gtgaatcagc atgaaatcag cgttcagtga
    25201 gtcggcctcg gcaaagctct tcttcacgct gcgccacagc ggggcagcta tcatcttctt
    25261 catttcacac ctgtaaatca gtgtggtgct gtcctgtgac gcctgcccgg caggggtcag
    25321 gacagaggaa tctgaagccg cttcattccc cggcgtcacc ggtcgggtgg cgctgccaga
    25381 aattgctgct atggtgcaca gattaagaat cagaacggag agtatctttt tcatggggtt
    25441 atctttagtt ccttaatgta ttcaatgaat tttgccagag cctccctttt cctgtcatca
    25501 aggcggaagt cgatgttgtg gttgaggtaa aagataacct cttcaggagt ggctgaggag
    25561 ccctccttca ggagtgagac tgcttcattc cttctttcga gcccatcgct cagcgccttg
    25621 ttgaaaatag cggtgaattg ttccggcaat cgccgtgttg atacccagca ggcaaataca
    25681 aaaggcagcc ccgtaaattt cttccactcc aggcccagat cgagtttgta cctgaagtgc
    25741 ctggcatatt cgaaacagcg gtcgccgatc atcacggcag cctcaccgtg gctcatggcg
    25801 gtgatgtcaa actcctcagg tgccggaagc cacctgtact cacgcttcca tgctttcgcc
    25861 gcgagaatac ggcacagggt tacggagctc cgtgaacggt aatcaagata tatggtactg
    25921 atctcttcaa acggggtgtt gcttacgagc agtacagtac gcacctcccc gttggtgctg
    25981 aggcagtaat cacctacaag atgatattct ttcatgcctg caatcatcgc cacgggtatc
    26041 aggccaaggt ctgtactgcc agcaatgagc ctgtcagcac actccgaagg atgacacaca
    26101 ccgagatcaa tcattcctgc aacttcaccg tcacggaggc caatcatgaa cggatagctg
    26161 tttatatact ttaccgctga aacccttatc ttttccatct cttttattct tcccgcagat
    26221 aaatgcaaag gttcaatttt ttcagaccag aacaaaatac tatttttgat ttttaaatca
    26281 gaggtaaaac cgggttgagg agagatagat atggaccgtt taatggcaat ttcaccggtc
    26341 gacggccgct acggtgagcg ggtagcagag ctggtgccat tcttttcgga gtttggcctg
    26401 atgcgctacc gcgtgctggt tgagattgaa tattttatag cgctgtcacg ggctggattg
    26461 ccgcagttcg aaggttttcc cgctgaaaag gaaaaggaac tgctggctgt atggaactca
    26521 ttctctctgg ctgacgctga gtttatcaag cagaaggagg cagtcaccaa tcatgatgtc
    26581 aaggctgttg aatacttcgt caaggagcag gtctcagcca ttggcctgga gaaatggtca
    26641 gagttcgttc atttcgggct tacgtcgcag gatgtaaata acacagccat gccgatggcc
    26701 atccgtgact ccgtaaggga ggtcatgaaa ccccttcttg tctcactgcg cgatgctctg
    26761 aacaagatgg cagatgagtg ggctgacata cccatgctgg cacgcaccca cggccagccc
    26821 gcatctccca caagactggg caaggagatc gccgtatttg ccgagaggct cgacagtgag
    26881 ctgttgacgc tgatgacgat gaagtacagc ggcaagttcg gcggagctac cggtaacttc
    26941 aatgcccacc gggtggcata tcccggcgtg aactggccgc aatttgccga caggctgctg
    27001 aatgacaggc ttggcctgga gagacagcag acaacaaccc agatagaaca ctatgacgga
    27061 ctggctgcgc tgtttgactg catcagcagg atcaatacca ttattaccga cctgtgcagg
    27121 gatgtctgga gctatatctc actggattat ttcaagcaga caatagtgaa gggcgaggtc
    27181 ggttcatcgg ccatgccgca caaggtaaat cccattgact ttgaaaacgg cgaggggaac
    27241 cttggcatgg caaacgcact tttgcggcat ctctcctcca aactgcctgt ctcgaggctg
    27301 cagcgcgacc tgacagactc aaccgcaatt cgcaatacag gggttgctat ggcccatacc
    27361 ctgattgcct ggaagtcagt taagaaagga ctgtcgaaac tcatcgtcaa ccaggacagg
    27421 attggcagag acctcgggga gaactgggtt gttgttgccg aagcattgca gactatactc
    27481 cgaagggaag gctacccgaa accttatgag gcgctgatgg ctctcacacg caccaatgaa
    27541 aaaataaccg gtgagacaat cagactattc attgataccc ttgatgtcac agaagccgtc
    27601 agggaggaga tgaaggccat taccccgttc aactacaccg gattctgata cgcggagatg
    27661 aagagcatca ggatactttt acagtacctc aggccctaca ggtggcttgc ggtgcagaac
    27721 atgggattca atatcctcag cgcttttttt gctctgttta cctataccct tatcgtaccg
    27781 tttctccaga tactgttcag cagggtggct tccgttcccg atcccggacc cttttccctc
    27841 tccgcatcgt acctgaaaga ctggacaaac tggtttttct cctcgtctat cgagcgatac
    27901 ggagaaatga gaacactcct catcgtggtc ttcgtctttg cggtggccag ctttatcaag
    27961 aaccttctta tcttcctggc caacaactgt atggcaagtc ttcgggcagg aaccgtcagg
    28021 gatgtcagga accgggtata tgacaagata ctgcgcctgc ctctctctta tttttcggag
    28081 gcccgtaagg gcgacctgat gacaagggta tcaaacgatg tgcaggagat agaagtctcg
    28141 gtgatgtcat cgctctccat gctcttccgc gacccgttta ccattattat tttcgtcatt
    28201 tacctgtttg ttaccagccc tcagctgact cttttcgctt tgctgatgct gccgttgagc
    28261 gggtgggcca tcgcccgtat cagcaggagc cttaagagcg cttcattcgt tggacaacaa
    28321 gcgctgggaa ggctgctttc agtgctcgag gagacactca ccgggcttcg catagtaaag
    28381 ggattcaatg cagaggacaa gatgaggagg cagttcggcg tggccaatga aaaatttacg
    28441 aaaaccttca ggagggtggc acgcaaggct tatctcgccc acccggtaag tgagttcctc
    28501 tcgactgtgg tgctgctgat tgtcatctac tacggaggat tcctcgcact caggggcaca
    28561 ggaaacatgt cgcctgacag gctcatcgca ttcgtggtcg tcttctcaca gataatacag
    28621 ccggcaaaag caatatcaag cgcatggttc agcatacaga aggggatggc ctcaattgac
    28681 aggataaacg aagtgctcaa cgccggcgag acaataacac agaaacccga tgcagtccgg
    28741 ataacaggct ttaatgacag gatagagttc agaaatgtgt ggtttgccta taacaatgac
    28801 cccgtgctga aggatatctc cctcacaatc aggaagggac aaactgttgc cgtagtgggc
    28861 cggtcgggtg ggggcaagtc aaccctggct gacctcatac ccaggttcat tgaccccgac
    28921 aggggtgccg ttctcattga cggagcagat gtgagggacg tcaacattag ggatctcagg
    28981 caactgatgg gtattgtgag tcagcaggcg atactgttca atgatacctt ttataataac
    29041 atttcctttg ccctcgatga ggtcaaagat gaggggagcg ttgaacgggc tgcaacaatt
    29101 gccaatgctc acgccttcat aactgagaca cctgacggtt accagaatac cgttggcgaa
    29161 ggtggcaaca aactaagtgg cggtcagcgc cagaggataa gcattgccag agccataatg
    29221 gctaatccgc cgatccttat tcttgatgag gccacctcgg cgctcgatac cgagtcagag
    29281 cgcctggtgc aggacgccat gcttaagctg atggagaacc gcacgtcggt aatcatcgcc
    29341 caccggctct ctacaataca gcatgcagac atgatagtgg tggtggaaga tggccggata
    29401 gaggagtgcg gaacacatga ggagctcata aggaatccaa ccggcatcta caggaaactg
    29461 tatgaaatgc aaacctttta agcaatggag atgtgcgatt tgcgatttgc gatcttccac
    29521 gcaggggata aagctttgta acagtatcaa cgcaaacaaa accatccgga cattgtttaa
    29581 tcaacaacag acaaatcaac ccatattgaa taatggagac taaaaccacc acagacattc
    29641 tgaaggaagc catcctgctt gagcaacgcg gcagggcttt ctattccagt gtagctgcac
    29701 agacagccag cgaggctgtg aaacagattt tcacgatgat tgcaaatgag gaagaggagc
    29761 acattcgctt tctctctgaa cagtatgccc attaccggaa gcacaatgta ttcggtgatg
    29821 ttacagtgca tcagggcgat gctcgtgacg aggtgaccag gtctgtcctc tcggaagaga
    29881 tgaagagcca gatcagcgct gccggcttcg aagccgcagc aataagcgca gcgatggatt
    29941 tcgaggcaaa ggctgtggag ctatactcat cacgggctga gtcagccaat gatccaaatg
    30001 aaaaatcgct ctacagaatg cttgctgaat gggagagagg gcatcatcag tggctggcac
    30061 gcatcgacaa ggagttgagg gagcaggtct ggtatgacaa taacttctgg cccttctagt
    30121 caagctttac catacgcctg aattttgcag gtatctttga ttcgaaacgc atctccttac
    30181 cggtaacggg gtgaatgaat gccagtacgc tggcatggag acccagcctt ccgatagggc
    30241 ttgaggtggc gccgtacttt ttgtcgccgg ctatactgtg gcctatctcc tgcatgtgaa
    30301 gccttatctg gttctttttt cccgtttcca gtgtcacctc cagcagagaa tattcctttc
    30361 cgcttttcag ttttctgaat cttgtgacag ccagttcgcc tttttccggg ttgcgtgttg
    30421 agtgcattac aagtgccttg ctctcatata tatatgattt tatgacgccc tccggctcgg
    30481 tcacctcgcc ctcgacgagg gcgacatagg tgcgtgaagt gacgttgcgt ttccagtcac
    30541 gctgcatgag gctctgcacc ttttcgcttc gcgcaaacat catcagtccc gaggtatcgc
    30601 ggtcgaggcg gtggacgatg aatatccggt ttgacgggtt ctggctcttt acaaatgtgc
    30661 tgagaatatt ataggcggtg aggtactttt cgttgtccga agccatcgag agaagtcccg
    30721 catttttgtc aatcaccacc aggtgctcat cctcgtagac caggcgcatg ttgcgtgcca
    30781 tcagtccccc ggtatgtctt gtggcggaga tagttattga gtctcctgtt ttcagttcat
    30841 ggttgaactg ggtcaccggc tcgccgttga ccagtacctg gcggttacgg agaagtgact
    30901 tgatgttgtc acggctcttg tttttcagtg agctgatgag gaagggcatc agtgttgtct
    30961 gcccggtaac ctcgagaggg atgtcagttg tcatatgcgg agtcttggtg caaagatagg
    31021 ggagaatatg ggattacctt cggtgatccc tgtccggggg ggcgcgactg acaattcagg
    31081 catgcgcaga ggtgtgataa ccttcggtga tcaggggatt actctccgcc ggctggcgga
    31141 tcgtgaaccc tgtccggggg gacctgcaaa ccacagcgct ctcacgggct gcgcccatga
    31201 gatctttatt ttccgttatt gctcgctccg gcaagccagg ctcgcactgc cgtaaaataa
    31261 aagtcccttc cgcctgcagg cggaagggag cgctgtgttt tgcggagaaa gagggattcg
    31321 aacccccggt ccagtcgccc ggacaacggt tttcaagacc gccgcaatcg accactctgc
    31381 catttctccc tttgcatccc tgactcaccg tgggtgacgg gcgcagcggg tgcaaatata
    31441 atcttttttt gcttttctgc cccggagaca aaaatttaac cggaatgctc acctggcacc
    31501 atcctctgac cccccgtaaa tgggcgcctg acactatcac cgagcctctg ttcaccatct
    31561 tgcgcggggt tattaacaaa aaaagtgctc atttctcata aaatccggcc tctcaggcat
    31621 aaaaaagatc ataaaaactt gcatttgaat aagaatggaa taaatttgta tgcaattaga
    31681 gattttggtc taaccctaaa ttgaattgac tatgacaaaa aaagaattag taggcagcat
    31741 tgcaaaaaaa gcaggacttt cacttaaaga tgccaacatc gctctgaacg ctttcgtagg
    31801 cgcatttggt gatgccatga aaaaagggca aaggctccag ctcccctcaa tgggtacttt
    31861 caaagttgag aagagaagtg caagagttgt tcgcaacccc cgcaccggcg ccaaactgaa
    31921 tgttcccgcc aagaaagtcg tgaaattcaa agcagctccg gctcttacaa aaggcctcaa
    31981 gtaaaagttt caggggtgct caggcacccc tttttatttc cttatcctga tacttccttg
    32041 gtccgtatct tttgtatggg atcaaaaacc ggacaggaat tctttttgcg aatattttag
    32101 tatatttgtt aacgaagctt gaacccaacc gtgtattccg gaaatgaatt gtattattgt
    32161 tgatgatgat cccgtatggc tccgaactct tgaggaattc gtgggtaaat cttcttccct
    32221 ctccctcgtg ggaagtttct ctgattccct gtcggcacgc aacctgctga tgtcccgaag
    32281 ggatatcgaa cttatctttc tcgacatcca gatgccggag atggatggat tcgattttct
    32341 gtccagcctc gaaaaccctc cgcacatcat atttgtgtcg ggaagcgaag aatacgccta
    32401 caaggctttt aactacaacg gcatagacta ccttctcaaa ccgattacct acccgcgctt
    32461 ctgccgtgcc gtggagaaaa tcatgaaata ctacgcaccc agggcggtac cgtcagccgc
    32521 cggagaggag gagatcttca ttaaaaaggc ttcttcgctt gtgcgtttga aactcaagga
    32581 gatactctat gttgaggctc ttgagaatta tgtcagcgtc attacacgag acgagaaata
    32641 taccatccac tttaccatga gggccataga acagcaactg ccctcaagcc tctttgtgag
    32701 aatccacagg tcgtacctcg taaataaaag cgttatcagg tcgatcagcg aaaacagcct
    32761 tacaatagta gtcggcagca atccgaagat cctgcccgtg ggcaaatcat accgcgacgg
    32821 gataatgaac tacattaata taatgcagcg ctgatgcctt cgcaaaggca actttttctt
    32881 gatcatatcg gacaaacctc tccctcaccc ctcttgcttg agattgaaag ggcggaaggg
    32941 tcattcctgt atgacagcag cggccgcgat attcttgacc ttatctcagg cgtctccgtg
    33001 agcaacaccg gtcatcgcca tccggcagtc atggctgctc tgaataagca ggccggaaag
    33061 tatatgcacc tcatggtcta cggcgaggtg atacagagtc cccaggtgca gtacgccgca
    33121 cgcctcgcgt cacttctgcc cccctcgctg gaatcagtct attttgtcaa ctcaggcagc
    33181 gaagccatcg aaggagccat caaactggcg cggaggtaca ccggtcgcaa tgagatcatc
    33241 tatttccgca acgcgtacca cggatccacc accggggctc tcagcgtaca gggctcggag
    33301 gagtaccgga atgccttcag gccgctcatg cctgccacaa ccatggctga gttcaacagc
    33361 catgaggctg tgcagatgat cagcagcagc accgccgccg ttctcgtcga gcccgtgcaa
    33421 gccgaagcgg gtgtggtgca gccggaagac agcttccttg ccggacttcg aagtgcatgc
    33481 tgcaggcacg gcgccatgct gatattcgat gaggtgcaga ctggattcgg ccgcaccggc
    33541 agcctcttct cacttttcag gtacaatgtc actcctgaca tcctggtcct ggccaaggcg
    33601 ctcggcggag gactgccact cggagccttt atcgcttcgc gcgaaataat atcatcactg
    33661 acccgtgacc cggtgctggg gcacataacc acctttggcg gacatcctct ctgctgcgca
    33721 acaggactgg catcgctgga agtgattgtc agtgaagatc ttgcagctgc tgcacgggaa
    33781 aaggaaaagc tgttcagaga agaactggca ggaatgcctt tcagagagat aaggggcgaa
    33841 ggcctcctcc tggccgtggt gccggaaaag ccggagatta tcccggctat gattgcccat
    33901 gcaccggaac acggactgct gctcgattat ttcctcttct gttccaccgc ctttcgcata
    33961 gcgccgccgc tcaccatatc agagaaggag atcagcctgg cctgccagag actgaaaaag
    34021 ctggtcaggg caacagacag tggggaaggt ctgagggtct gagggtatga aagtcggaaa
    34081 gtaaatctca attcgcaaat ctcaattcgc aaatatttca ggcctgccat ggcctgactg
    34141 acactatgtc agaattcctg ctgcggcacg tcctttgaac aaaaggggca ggataatcag
    34201 aaaaccaaaa catatggaaa agggtaaaat cggagtaact acagaaaaca tctttccaat
    34261 catcaagaaa tttctctatt cggatcatga gatcttcctg agagagctgg tatcaaacgc
    34321 cactgacgcc atccagaagc tgaaggcact gtcctcagcc ggagaattca agggcgagct
    34381 cggtgagctt cgcgtaagcg tcacactcga tcccaaaaag aaaaccctca ccgtctccga
    34441 caacggtatt gggatgagcg ctgaagagat agacaaatac ctgaaccaga tcgccttctc
    34501 gagcgccaac gaattcctcg acaaatacaa ggatcaggcc agcggactca tcggccagtt
    34561 cggcctcgga ttctattcgg cattcatggt ctctgacaag gtcgaagtca tatcaaggtc
    34621 atatcgcggg aacgtgcctg ctgtgaagtg gacatgcgac ggcagcccgg aatactcaat
    34681 cgaagagacg aaaagggaag agcacggaac ggacgtaatc cttcacatag acaaggagtc
    34741 aaaggagttc ctcgaagaga accgcattga gggcctcctg aagaaatact gcagcttcct
    34801 gccagtgcag gtgcagtttg gcaatgagaa ggagtggaag gacgggaaaa tggtcgatac
    34861 cggaaagccg aagatcatca acaacaccca gccggcctgg acacgcaaac ctgctgacct
    34921 gaaggatgag gactacgcaa gcttctaccg cgagctgtac ccgatgggcg aggagcctct
    34981 cttccacatc cacctgaacg tcgattatcc gttcaaactg accgggatcc tctacttccc
    35041 gaagatcagg aacaacttcg aaattcagaa aaacaagata cagctgtact gcaaccaggt
    35101 attcgtgact gactcggtgg aaggcattgt ccctgacttc ctgacactgt tgcacggagt
    35161 gatagattcc ccggatatcc cgctgaacgt ttccaggagc tatctgcaga gcgattccaa
    35221 cgtcaagaaa ataagcgggc atatcacaaa gaaagtggcc gaccgcctga cagacatctt
    35281 caagaaggac agggaaactt ttgaaaagaa atgggatgac cttaaattgt tcatcgagta
    35341 cggcatgatc accgaggaga aattctttga aaagatatcg aagattactc tttataaaaa
    35401 tactgcaggc aagtacttca ccctcgagga gtatgacaag atcatcaagg aaaaccagac
    35461 cgacaaggac aagtcgctgg tgcacctcta tacaactgac cctgttgccc agcataccta
    35521 tgtggctaaa gctgcagaaa agggttatga cgtgcttgtg cttgacggac agcttgatac
    35581 acatctcata aacaccctcg agtcaaagga gccttcatca aggtttgtaa gggttgacag
    35641 tgacgtgatt gaaaagctta tcaaaaagga cgagagtcat gcctcgaagc ttacccagtc
    35701 acagtcggat gacctggctc aggtattccg cacccaggtg gctgacactg acaaggcacg
    35761 gttcaatgta agggctgaga acctcgatga aaatgacctt ccgatggtta tcacgcagag
    35821 cgagttcatg cgcaggatga aggacatggc gcagttcgga ggaggttatt ctttctacgg
    35881 cgaaatgcct gacagtttcg accttgtgct gaacaccaac catccgctgg ttatttcatt
    35941 ggccggggac ctcgacaagg agcatgccga cgagttgaag aagaacggtg cgggaataga
    36001 aaaaacgaag gttgagattg agttcatgga aaaggagcac ggcaagaaga agcccgaaga
    36061 ggttctttca gttgagaagg atgaccttga gaggctcagg ggcgagctga agcagctcga
    36121 ggagaagagg aaaaccctgc tgggcggatt cggcaatgat caccaggccg ttaggcagct
    36181 gatagacctc gccatgcttg caaacaacat gctcaagggc gaagcgctca gcgcattcgt
    36241 aaagcgaagc atcagcctcc tctagaagta attttaaaat attgccttac atattgacgc
    36301 cccccccggg ggcgtctttt tactatccca ttatcctcgc aagctcagac ttgatgtatg
    36361 cgatggcagt acgggttggt tcggtttcca tctccacaac ttcctcgagg agcagcctcg
    36421 agagctcttc gcctgtggcg gcaggaggaa gctgagaggc aacgctgttg atcagcctga
    36481 caatggtgcc gcgcgcattc ttcaccatct cccatgcctc actgctcata tagatctgct
    36541 gcgagaggtt gtgctcatat tcgctcctga cagcagtgac cagcgctgcc tggagctgct
    36601 gggccgtcat ttccggtctc gcagtgcgta ttatcagcga ctccggagaa atgcgctcga
    36661 ggaacagcac caccctctca tatgcctgca gcctgaccgg ggtgagggtt ttcacggtct
    36721 gcagcagcag atcctgcctc cgccgctcct ggtcgtcacg cagcatgttc cttattacca
    36781 gccatgcggt tagcatcacc agaagtgcag gaatggttat cttgagtatc tccaggaagg
    36841 tttccatgat cgcagttgtt taaggtcttt gtgcgatgca aagtaatcaa ttttcaggcc
    36901 ttgttgacgg tctgaacaaa gatttggaag gattattcag tcttgccagc ccggttgcaa
    36961 tgatttccgc aacgccccgc caccctgcta taatcccttt tctttttcac ttttaacaat
    37021 tacatttggc ctttccggta acga
//
LOCUS       DAOPXU010000017        36397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_1052_length_36397_cov_13.077734, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000017 DAOPXU010000000
VERSION     DAOPXU010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 36397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36397
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1052_length_36397_cov_13.077734"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..1016)
                     /locus_tag="PKK03_02960"
     CDS             complement(42..1016)
                     /locus_tag="PKK03_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNX83383.1"
                     /translation="MASLSDIKKPVAAEMREFESYFRHTLQSDIPFFHVILDNFLRRK
                     GKQMRPLLVFLTARLNGKVNETTYIAATFIELLHTATLVHDDVVDDASERRGLPSINV
                     LYNSKIAVLVGDYLLAQGLLISVEKQRFNMLGIVSDAVREIVKGELVQLQKTRKLDIR
                     EEDYFRIIKMKTAALISACTACGASSVTDDPDAIARMKEMGENIGIAFQIKDDLLDYT
                     GEGLTGKREGNDIKEKKITLPLIHALRETGPIERRHIIRLLRKKKKGSREIREVIGFV
                     KKHGGLDYAEAAMKEYSDRAIRILEEYPDSETKSALIEFVIFSTLRRK"
     gene            complement(1111..1722)
                     /locus_tag="PKK03_02965"
     CDS             complement(1111..1722)
                     /locus_tag="PKK03_02965"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HNX83384.1"
                     /translation="MSNRTKALLLIFSILIVDQIVKIIVKTNMVIGDEIPVLGNWFRI
                     HFLENNGMAFGMEWGGKTGKVALSVFRMVAIVGISWYLDSIIKKNAHTGLILSVSAII
                     AGAAGNLIDSAFYGMIFSESWHTPAVLFPEGGGYSSFLHGRVVDMLYFPVIDSHWPDW
                     SPFRPGQTLIFFRPVFNLADTSITTGVLAILVFQKRFMGEKKS"
     gene            complement(1819..3459)
                     /locus_tag="PKK03_02970"
     CDS             complement(1819..3459)
                     /locus_tag="PKK03_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX83385.1"
                     /translation="MNNNEMLTIPFLAGESFRKHGKNNAMGFVGEDVITYHQLKERTR
                     SVMRLLEDLRITPGDKVAILSTSMPNWGAAYLAITSMGAVAVPILPDFTPGEIANVLN
                     HSEAKALFLSGNLAHKVAEFKPEALETVIAIDDFRITSGNDQVLFVAGSEPSGKYNVA
                     EEDLAVIIYTSGTTGKSKGVMLTHRNICFTARQVMTIQYVVESDVFLSILPLSHTYEQ
                     TLGLILPLFNGAMIWYLRKAPTPSVLLPALQQVRPTTMLTVPLIMEKVYFNKVAPTFH
                     KNKVVAFLYGTPLFRRLLNRIAGKKIYEAFGGRLRFFGVGGAKLNPTVEQFLLDARFP
                     YAIGYGLTETSPLLAGTKVGSCRHGSTGPAMQGVTLRIKNPDPVTGEGEIWARGENVM
                     KGYYREPAMTAEVLTPDGWFRTGDLGVVDKHGYLSIRGRLKNMIVGSSGENIYPEEIE
                     SVINDFGHVIESLVVQKKGKLVAMVHLNIEELQAKYAQMKKDFADQAELIINEYLEEL
                     QRYVNSQVNRYSQLQLVVYQPEPFQKTATLKIKRFLYY"
     gene            3602..4051
                     /locus_tag="PKK03_02975"
     CDS             3602..4051
                     /locus_tag="PKK03_02975"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNX83386.1"
                     /translation="MALIRVTKEFRFEMAHALWNYDGPCRNVHGHSYILYVTLAGEPV
                     NDPDHPRNGMVIDFGDLKKIVRENIVDRFDHALMVSGLAPAATVEAYRQHFGNVIVSP
                     YQPTCENLVADIAATLGGRMPEGTYLHSIRLFETATACAEWHASENR"
     gene            4048..6891
                     /gene="polA"
                     /locus_tag="PKK03_02980"
     CDS             4048..6891
                     /gene="polA"
                     /locus_tag="PKK03_02980"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNX83387.1"
                     /translation="MKETDNDRRLFLLDAYALIFRSYYAFIRNPRVTSRGMNTSAIFG
                     FLLTLEDLLSKQKPSHIAVVFDPPGPTFRNEIYPAYKANRDATPEDIKIAVPYIKRLL
                     EAFRIPVIEVPGYEADDVIGTLARKAAEQGYRAFMMTPDKDFAQLVTDNVVMYRPGRS
                     GGEAEIWGREEIVREYGVPPEHITDLLGLMGDTSDNIPGAPGIGPKTACKLITEHGAV
                     ESLLENTSALQGRQKDIIEQNREQILLSKRLATIEQDVPVEFRHAGFHRQETDTEALR
                     KLYEELEFRTMASKLPAPGTRQTARTDLLSQSAGDTAAQDAPPAAAMPKTGESQGSLF
                     GEISSPAGKTSANIGTAPHRYTLIETEKESARLASELSKLSDFCFDTETNSLDPLNAQ
                     LVSIAFSWEKGSGTMLWLPPEKDEALKLLEPLRSVLENEKIRKTGQNLKFDIQVLAGY
                     GISVRGPLFDTMLAHYLLEPDMRHNMDLLSEQYLGYEPVHIEELIGERGPRQKTMREV
                     DRERVKEYAVEDTDVTWQLMKLFEPMLIKQELDTLAAGIEMPLIRVLAAMERTGVLLD
                     ESVLKKYAVTLRENIIRLEQEIYTFAGHEFNISSPKQLGDILFVRLRLDDNARLTKTK
                     QYRTDEEVLQRLTGRHPIIEKVLEYRGLKKLLSTYVEALPQLVDPGTGRIHTTYNQAV
                     ASTGRLSSTNPNLQNIPVRDSEGREIRKAFIPANGKIFLSADYSQIELRLMAHLSGDS
                     AMISDFLSGQDIHAATASKIFGVPVADVTREMRARAKTANFGIIYGISAFGLSERLTI
                     GRKEAKELIDGYFSSYPGVKAYMDESIRRARELGYVTTMFGRRRYLPDIHSRNQVVRG
                     NAERNAINAPLQGSAADIIKIAMIRIATRLEREMPGAKMILQVHDELIFEVDESEVKK
                     LADLVMEEMKGAAQLSVPLEVDTGTGYNWLEAH"
     gene            6894..7130
                     /locus_tag="PKK03_02985"
     CDS             6894..7130
                     /locus_tag="PKK03_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNX83388.1"
                     /translation="MAAEVRILYFGAAQEATGKSDETIVAGDTASVRNQLISRYPALK
                     QIPFRMALNRTLLKGESELQKSDIIAIMPPFQGG"
     gene            7148..7549
                     /locus_tag="PKK03_02990"
     CDS             7148..7549
                     /locus_tag="PKK03_02990"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNX83389.1"
                     /translation="MKNRYLVGGPITPILISELMTLGNDDAGAGAVSIFIGRVRDDET
                     DGKRVTAIEYSAYEEMAVKEAEEVITTVKSAFSDVRSVIIVHSLGVVKAGEASLFIMV
                     TSGHRDHATRACRHALEMIKERLPVWKKELY"
     gene            complement(7623..9296)
                     /locus_tag="PKK03_02995"
     CDS             complement(7623..9296)
                     /locus_tag="PKK03_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX83390.1"
                     /translation="MNSKNIKKIVLVPVIAVTAVITLGFLLGGQENKDFRLTKNLDIY
                     ISLFRELNAFYVDEIDPEKLVTRSINSMLATLDPYTVYYPEEETGDLDFMTTGKYGGF
                     GSLIRKSGDYILVTNVYQGFPADKSGIRPGDLMISIDGTSLKGVSSDKASDMLKGEPG
                     TEAEIIIRRNGQDITKKLRRERIAISAVPYYGMVDENTGYIRFTNFTQNCIAEVREAL
                     IDLKDKQGAEDLILDLRSNPGGLVNEAVEIVNLFVKPGQEVVSTRGRAKQYDAVFRTN
                     RTPIAPDMPVVVLINRGSASASEIVAGALQDLDRGVIVGERSYGKGLVQVARPLSYKA
                     QVKMTTAKYYIPSGRCIQAVDFSHRNEDGSVGAIPDSLTKTFRTKSGRPVRDGGGIIP
                     DVTVPSDMLNRFAGELFVQNMIFDFATEYYWSHPRPPVLDSLKITENDLDQFTNFLAG
                     KKFSYRTDSESILEELTKTAMEEGLYDSNREAMEKLKSGLSHTLEREMTLYRPDVTEL
                     IESELAGRYFYDWGMVEYSVTRDTQIKKAIDIAGNRAHYASLLKAPAGI"
     gene            complement(9316..9708)
                     /locus_tag="PKK03_03000"
     CDS             complement(9316..9708)
                     /locus_tag="PKK03_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNX83391.1"
                     /translation="MKKREASFRKGERLCGVRAISELFAEGRSLNLPSLRVICRFTPA
                     IEGLHPVRVLITVPKRHFKKAVDRNLMRRRIREAWRKNKEPLVMLMKQAGRHADIAII
                     WTDTSIRPCEFTEKCITEAISRVSGLKY"
     gene            complement(9775..10524)
                     /locus_tag="PKK03_03005"
     CDS             complement(9775..10524)
                     /locus_tag="PKK03_03005"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNX83392.1"
                     /translation="MKIRKVLVSQPEPNNPKSPYFELAKKYNLKIDFKQFIQVEGISA
                     KDFRQQKINLSDFSAIIFTSKTAIDHYFRICEELRVTVPDTMKYFCITENVAFYLQKY
                     IVYRKRKIFHGKANFEDLIDVIIKHKEENFFVPLSDPHKSDIPELLARNRIRHTIGTM
                     YRTISRDFDSKELDYDLIVFFSPSGIKSLKENFPTFVQGEVKIAAFGPTTAEAVTNEG
                     FRLDLQAPNPKAPSMTMAIDNFLKEFNRNNK"
     gene            complement(10567..11424)
                     /locus_tag="PKK03_03010"
     CDS             complement(10567..11424)
                     /locus_tag="PKK03_03010"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HNX83393.1"
                     /translation="MTNGPGDTISVAADTLNLPVRQTEFSIGRIIPLISPAEDFAVEE
                     KSVTALTADYISGLKRVDGMPRPPGPVNSDMSFIILSLSLLLITILTVFGRRSIITGL
                     SSISFRRHEETIPVGTSEVFSWPPVLRNIFTILNVSLFASTALLVTGLAGRDLFNGPV
                     GLTAIFAGSFLTALLLRHLSCIALAGISGLKSLFREYMNVVYNSWFACSFFLFILNGL
                     ILFAPVQNPLPFIIAGLIITAIFLLTRALRLLYIFHNRHVSIFYYILYLCALEVLPVM
                     VILKLLGVY"
     gene            complement(11434..12306)
                     /gene="deoC"
                     /locus_tag="PKK03_03015"
     CDS             complement(11434..12306)
                     /gene="deoC"
                     /locus_tag="PKK03_03015"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNX83394.1"
                     /translation="MSKLYKKLLNNDYSPGEMGECIAEAKRTPGTVERAPILREIFSF
                     IDLTSLNTSDSDATIRSFAERTAQFSYHFNDISNVAALCVYPNFVKVAHEGLGGCGVR
                     LAVVAASFPTAQTFTEVKILETSMAVRMGADEIDVVIPVGTFLKGDYDAVIDELKAIR
                     EAAGDTTLKVILETGLLKDERNIYRASIIAMESGADFVKTSTGKSSVSATPEAAWVIC
                     RAIRDYYNETGIMIGFKPAGGIVTPDDAIIYYFIVKNMLGAKWLTPEYFRIGASRLAN
                     NILSELYPGKPAYF"
     gene            complement(12402..13778)
                     /locus_tag="PKK03_03020"
     CDS             complement(12402..13778)
                     /locus_tag="PKK03_03020"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX83395.1"
                     /translation="MTEKELKRSVLSVTAFASFLTPFMGSSVNLALPSIGEELSLNAV
                     TLNWIISSYMLTTSMLLLPAGRAGDILGRRKVFTAGMMVFTLSMILLTFTPGIRWLIG
                     TRILQGIGGAMMFGTNMAILTSVFPPGERGRAMGINVTAVYIGLASGPFLGGLLTRYL
                     GWRSIFAFLIPMGIISLILIRTKMKREWAEAKGESFDWAGSVIYGISMAALMYGFSRL
                     PSAEGWAITATGVVLMPLFIMREKKAVHPLFDVTLISHNRIFAFSSLAALIHYAATSA
                     IGFFLSLFLQYIRGLGPREAGLVLMSWPLTMALFSPAAGKLSDKYNPGVLASTGMGIT
                     SAGLIMLCFLTEQTSVAFIVTVLVIMGAGFSLFSSPNSNAIMSSVEKRQLGNASGMLG
                     TMRNVGQTLSMAIALMLLAIYMGQEKINPDNYPQLMNSMKVGFIVFSILCFAGIFASL
                     ARNKSLRK"
     gene            complement(13775..14227)
                     /gene="dtd"
                     /locus_tag="PKK03_03025"
     CDS             complement(13775..14227)
                     /gene="dtd"
                     /locus_tag="PKK03_03025"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNX83396.1"
                     /translation="MRIVVQRVKRASVTIDGKVHSSIGKGLLVLAGFEDADTAEDINW
                     MAAKMVNLRLFDDSIGVMNLSAVETGSGIMIVSQFTLHASTKKGNRPSYIRAARPEKA
                     IPLYEEFILRTAGLLGRTPATGVFGAMMDVELVNDGPVTIIIDSRNRE"
     gene            14288..14854
                     /locus_tag="PKK03_03030"
     CDS             14288..14854
                     /locus_tag="PKK03_03030"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNX83397.1"
                     /translation="MDLITITAIALGLSLDSFAVSLSCGVIESKIRFRNAVRIAFILA
                     LFQGGLTIVGFFLGSTVSDYISRYDHWVAFILLMVLGIRMIIGGLKVTSDRQPIDINR
                     LPVVLTMAVGTSIDAFAVGISLAFLKVQIWTAGLIIGTVTFLASMTAIRIGKSAGSRL
                     GSRVEIIGGIILAAIGVRILVEHLLSAG"
     gene            complement(14907..15695)
                     /locus_tag="PKK03_03035"
     CDS             complement(14907..15695)
                     /locus_tag="PKK03_03035"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HNX83398.1"
                     /translation="MIYGTDYHMHTVYSDGKREPEAYIEAAIRLGLKELGFSDHLNPL
                     GGDTGWCMDHDRLPAYAEHILRLKREYKDLIAVRLGLEIDYLPGTEKDTEKIINSFPF
                     DYIIGSVHYMGDETVDLGPEFYIGKDVDQIYENYFNLVCEAASTGFFDIMGHPDLVRI
                     HRFYPQADISLLYSMMASSFAIHEVAFELNTNGRNKPLHDFYPDRQYLHLFAQQGVAV
                     CVNSDAHFPERVGQYFGEAYDLLKRAGYREMAVFSGRERFMIPL"
     gene            15786..16493
                     /locus_tag="PKK03_03040"
     CDS             15786..16493
                     /locus_tag="PKK03_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNX83399.1"
                     /translation="MVVEANTPKRKCNCLSCELRNLVFTNLEEEDVRRVCESKEEFSY
                     AKGEIIHSEGDPIHYFKYLKTGLVKLFRLTPEGDEQVITITRPFEFVSNTNVFHEDKY
                     SYSLSALEDSVVCCVRIEFIKELILRNGKFALNLISSLSRTMESIISLGLEIRKRNLA
                     GRTAFVLLYFSNEIYHSRIFELPVSRKEIADLISMSSANVIRTFSEFRRDGIIRANGR
                     TIEITDMNKLEVISRRG"
     gene            16550..18481
                     /locus_tag="PKK03_03045"
     CDS             16550..18481
                     /locus_tag="PKK03_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNX83400.1"
                     /translation="MKKTTGAFFLFLFASVIYPVGAQRPVFPDSSVYTVEGPRHGVSF
                     FPRVHHPGVEYTPGDTLSFDRYHTYGVICHWMNRWAEDHPEIVTLYEVGRSYEGRPVM
                     QMTITSGKNGSDTDKPAAFFEGNRHSGEVTGAESVLWLARHLIEGYGNDPSVTDLLDH
                     FTVYLRPVNNPDGHELYLNTAQSNRSTVRPDDNDGDGLFDEDAPEDLDGDGIILTLRW
                     PDRKNGTLVPDPDDPSGRVMKRVPRGEGIYATSAEGIDNDGDGRINEDGIGGLDLHRN
                     YPENWRPEREQTGRGFSQRGAGAYPLSEPETRAVFTFLMTHPNIYVVNSMDTTVPMHL
                     RPPSTSPSEERMYPEDLKWYRYFDDLARKITGYEKAGDVYNDYGNGEPLFGHGPDFGY
                     WYYGAIWYGDELWNGGRPKDYDGDGDKDQLDMLAWDEEENSGEGFHEWKPVSHPVHDS
                     VEAGGFHPKFFSQNPPPAHLEKWASNQALFNKELISHLPRLEWEEYSVKRIKKYRTDS
                     VDYKLTIRFRNAGRLPTALSQAHLVKMVRPDEVKLKFEGDTAGIPRSRVIEPASPATG
                     RGNMMMSEERSRRPEYVTVAVGYTPGGATAEANFIIRVYGEAEVRGTATVETTRAGIL
                     PEKEFFLGNPTSQAGPQGR"
     gene            complement(18455..19357)
                     /locus_tag="PKK03_03050"
     CDS             complement(18455..19357)
                     /locus_tag="PKK03_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNX83401.1"
                     /translation="MHRVVITGGRGLIGSYLSTLLAAEGWLVVHLTRGGKKNGRYQSF
                     RWDPDSGYCDPDAFREGDAIIHLAAANIGDGRWTEARKRDIVNSRTVSGQLIHKMTVG
                     AGKIPSVFITASGVNYYGSVNSDRVFSESDPPAGDFLGETCRLWEAAADLFSGAGVRV
                     VRMRTAVVLARSGSALNRMTAPARAGLVVRFAPGEQYFPWIHIADLCSIYHKALTDTA
                     MSGPFNAVAPHHVTHDQLMSEVARQKRLPVFLPHVPAWLLRVVLGEMAVVLTTGSRIS
                     PDHLSSAGYRFRYPDISSALRACL"
     gene            complement(19361..19783)
                     /locus_tag="PKK03_03055"
     CDS             complement(19361..19783)
                     /locus_tag="PKK03_03055"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HNX83402.1"
                     /translation="MNKKDKALGYFREGYSCSQAVLMACTGGSRDAASAAAAFGGGMG
                     RLQKTCGAVTGAYIWFGMAHGVPGLPSESDKAAVYGRVKEFNRIFTVRNGTDQCSELL
                     GENLNTPEGKDNIARKGLSQKVCEKCIGDAIEIIETLE"
     gene            19904..21109
                     /locus_tag="PKK03_03060"
     CDS             19904..21109
                     /locus_tag="PKK03_03060"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNX83403.1"
                     /translation="MANQKKIKGVIGILTGGGDVPGLNPAIRAVTIRANREGYKVIGL
                     RRGWGGILEIIRDPKADNSQCFQVLTDDMVNRAGRTGGTFLHTSRTRPSHVAKNYVPE
                     HLKDTYKEEINDMTPEVLKNLEWLGIDYLIPIGGDDTLSYGVHLYKHGVKVVAIPKTM
                     DNDVPGTDYCIGFSTCVTRTIQMTNSLRTSAGSHERIMVLEVFGRYAGFTAMLPTMAG
                     AANRCVIPEYKFDIEQLTKLLVEDRNSNPSRYSIVLVSEGALYKGGDMIFQDSERDAY
                     GHAKLGGIGDLVSAEIKEHSAKFNNGKTINIINQKLGYMVRGGDPDAVDSIVPMAFGN
                     LALDLIMKGIHGRVVVLKNGRYDNVPIDVVTSSKKVVKVSEYYNVERLRPYYSNFEMK
                     PFFLMAAEY"
     gene            21195..22109
                     /gene="rsgA"
                     /locus_tag="PKK03_03065"
     CDS             21195..22109
                     /gene="rsgA"
                     /locus_tag="PKK03_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNX83404.1"
                     /translation="MKKGIVIKSTGSRYRVADEEGNIYECLLRGRFRVSGARTTNPVA
                     VGDHVTFDADEGVIVALMPRRNYIIRRASNLSRESQIIAANIDQAFLVITVKMPATPV
                     EFIDRFLATAEAYRIPAVILINKTDLYGEEEMARADLIKDVYEKIGYRVMMISATEDR
                     SLAEVEDALRNRITLLSGNSGVGKSTLLNRLNPELSLKTGSISDYHEQGKHITTFPEM
                     HSVADGGYVIDTPGIRGFGVIDFDRKEIYHFFPEIFRISSGCRFYNCLHLDEPGCAVR
                     EAVARNDIEPWRYRSYISMMLEDNEKYR"
     gene            22137..24956
                     /locus_tag="PKK03_03070"
     CDS             22137..24956
                     /locus_tag="PKK03_03070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX83405.1"
                     /translation="MRRTFIAAYIAFVLLMLITALFYISLYRNQIEYSNKLLDRQVMI
                     AGSDIDNTSMYIVSDLNEIDFSDDITLFFSDPGVNERAREKMKLYYSKYGEIIVGLML
                     YNTDGDVYTLFKDEERNSWLDGSYRAQTVPTIHTMDKLEPDRDRYRYFLPVLKDGEAL
                     GNLVITIDFRRYFSGIFAKYNLEKYQWQWVINDTGTVVFDNGGTNTSYAGVDRISESL
                     ARGNSGRLAHTMRTDGGEAEEILSSYYPVNIFGLDFCLVFSAHTEFFQKYIVRNTVIL
                     GLLTMLTLLFIIFLFGRFLRKQAKALETKTASEAGLLSILDQMPVGFIIYNEGREILR
                     ANRQAAGLFAFEDETAMAGKLMPDLSRNEYAMDIAGELGQGKVIRLPAPGGEKIIFLN
                     SIPVPYKGGNSTLDALIDITSLELARRQEADANIAKSELLARMSFEIRTPLNGILGMT
                     EMLNRSDLPEESKEVARLLRRSADLLLTIINDIFDVSKVETGKMILDEIPFRLRDEVA
                     YCINLIRRQNPEIPVRFESEIEQSVPENIIGDPYRLRQVITNLMTNSVAGTQSGLIRL
                     SCRVRKSDGNSFTLEFTLTDTGNTYTRAEIKRLFGDYITTISERSDWTEDLKLGPVLA
                     RQLTELMGGELTAESPAMKDTSGQEKGLRVTFTINVHLNEKISKNIELGMYADPGDIR
                     TLVITGPHGRDDDFLSIMHRVGLPVSVTSFQKHTASQIRAGLKSGTGRNILLVLFDEP
                     DNDGFEAAKVLMDEGLTSEHIVLMFTSRDPKGHYARCVDLGIDHLLVKPFAPEDLMSV
                     LREHFPALKELAARLNSAKTDVPVVLVVDDNYLNRKVVGSLLRVLGVGAEFASGGAEA
                     VDMAGEKSYDIILMDLIMPETDGFEASRIILGKNPSTQIVALSADNMPETRLKVEQSG
                     MKELLAKPVTVEELRRVIDRYHKPS"
     gene            24959..25996
                     /gene="mnmH"
                     /locus_tag="PKK03_03075"
     CDS             24959..25996
                     /gene="mnmH"
                     /locus_tag="PKK03_03075"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HNX83406.1"
                     /translation="MAFSPNKLPADEFLQLRASLPVADVRSPCEYAVGHVPGAVNIPL
                     FDDSQRAEVGTIYKREGNATAVIRGIDLAAPQMSGKLKSGLELAAGGRLLVYCWRGGM
                     RSEAMAWLFSLGGIEPLVLRGGYKAFRNHILGDLGRERKYVILGGLTGSGKTGILRYM
                     KSAGEQVTDLEGLASHRGSAFGALGQAPQPASEHFANLLYDDLSAHRAESPVWLEDES
                     RNIGTVFMPDCFFERMQSAPVIALMMSIETRMPRLVREYTSFPVEEIMASVMKISKRL
                     GGDRTREALDALKCGDYPTAIRITLEYYDKSYHYGLSKRPRDQVIYINTETDDEAVNA
                     ALAMEAAEGLT"
     gene            complement(25999..27261)
                     /locus_tag="PKK03_03080"
     CDS             complement(25999..27261)
                     /locus_tag="PKK03_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HNX83407.1"
                     /translation="MIRRNDIEVMAPAGSREALRAAIQGGADSVYFGVEKLNMRARSS
                     HNFTSAELPGIVALCREHGLRSYLTLNIVVYDEEVSEMKEVLASAAAAGITAVIATDQ
                     AAILAAREAGIEVHLSTQLNISNAGSLKFYSQWADVAVLSRELNLQQVKKIHDTIISD
                     DIRGPGGGPMKLEMFVHGALCMAISGKCYLSLHEYNASANRGNCYQTCRRGYTVSDRE
                     TGYELDIDNQYIMSPKDLCTISFLDQIIDAGARVLKIEGRARPPEYVKTVTRCYSEAA
                     AAVADGTFGQEKVEEWMRQLETVFNRGFWDGYYLGRKLGEWSTSYGSSATKRKIYLGK
                     VTNYFRKAGVAEIKLETGDLSAGDELVITGPSTGIVEITVKELRDASENEAETVLKGE
                     LCSVKTDGIVRRSDKVFRWVDASSLKNG"
     gene            complement(27274..28419)
                     /locus_tag="PKK03_03085"
     CDS             complement(27274..28419)
                     /locus_tag="PKK03_03085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNX83408.1"
                     /translation="MNIYRKRLLWKVLLLIFAVVIGLGSLIYTHDLVHRLKQEERKKA
                     EQWAEATRRLVTSDDFEFLFSIIEDNTTVPVILTDEEESIISQRNLPAALATDTLAMV
                     RELKRMAKRNDPISIELPNGIVNHLYYRESTLLRQLRVYPYVQLAVILLFIGVAYVAF
                     SASRMAEQNQVWVGLSRETAHQLGTPTSSLSAWSEIISSRYPGTDIAEELPADVDRLS
                     RVAERFSLIGSKPKLVPDNLPALAGATASYLRKRIASTIDLTVADETTGCTAVPLNAV
                     LLGWVIENLVKNSADAMKGTGQITVRLSENNAEAFIDVEDTGKGIPRKDFKTIFKPGY
                     TTRERGWGLGLSLSKRIVEEYHKGKIFVLRSEPGHGCCMRIMLKKQG"
     gene            complement(28423..31026)
                     /locus_tag="PKK03_03090"
     CDS             complement(28423..31026)
                     /locus_tag="PKK03_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNX83409.1"
                     /translation="MRLFRKIALIAAGLLLLLILVPGLYALLRQEHLTESLIRKVNES
                     VNTKISYGNLHITIFESFPNITVRFSDLLVEPSPFYDKKQLRHEDNDTLLYASSLSLT
                     VSMPSLLTGTVAVKSITARNGEVNLLTDRRGDINYEVFSEKNGDGKNVRLKNISAVDF
                     RAVYNDMSSGMRMAGRIGAATLGGELFRTGIYLSTALNAGIDSINLEGTTIRDIPVEA
                     GIKLRKSANSLSVAKGALSIADLRFDITGNVNYSSSTLNLIIEGRKISIASVISRLPD
                     RWKTVTGNFSPSGIIDLKCSITGPYGDAGKPHIDLTYGLAGGRMSHSSTGFRVNNLEF
                     SGALTNGDHNSAETFRFTVDKLTARYGSASVKGSFMMNNLRRPHITLNLDGDLDFDDL
                     DKIFRSGYIKDQTGTLSGSIRLSGTLPDSILPATALAYLSPDISLSFREFGATLARRG
                     LALKDVNGSLSVKNDLIADNLSFTFLDQRFTVNAAMKNFFPWLAGGGQNLDINGDVHV
                     DRLVTAALVPAKSKQPDSTGKPADIYPADVSANILLKADSLIFNNFRAANFSSRLTYR
                     PYVITFDEVYASGLEGVLEGELMLGRQQDGGFITKASLDVKNVDINQAFTSFNNFGQN
                     FIVSDNLRGRLTGNVTMLSPLDGNYRFFRKAIVAEAHLVISDGKLISFAPAESLSAYL
                     DLDELKNISFSKMENDIFINNGTVSIPKMLINSSAVNFTVYGTHGFEGDYIYHVRLLL
                     SEVMSRKARERNRNVSSFGQVKVDGSGKATVPLKIECVNGKTSVGYDFGQAQDNIKND
                     IAAEKQTLKGILNEEYGWYSADTTRTTPAESKPKFSITWEEGKEQPAATEPQKEEVKE
                     SPIKILLKKKR"
     gene            complement(31048..31767)
                     /gene="pepE"
                     /locus_tag="PKK03_03095"
     CDS             complement(31048..31767)
                     /gene="pepE"
                     /locus_tag="PKK03_03095"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HNX83410.1"
                     /translation="MRLLLISNSTNAGEAYLDYPKNNIRDFLGSRKVKALFIPYAAVT
                     FSYDEYEQKVRNRFNEVGHDVVSIHHFRDPVKAIMDAPAIVAGGGNTWMLLKCLRDNK
                     LIAPVRKKVLSGTPYIGWSAGSNVTCPTIMTTNDMPVTAPSSFRAFGLIPFQINPHYL
                     DANPAGHAGETREQRIQEFIEVNRELYVAGLREGTMFLLEGKNLSLIGPRNARVFRYG
                     SEPREVKPGSDLSFLLKISKR"
     gene            complement(31961..33010)
                     /locus_tag="PKK03_03100"
     CDS             complement(31961..33010)
                     /locus_tag="PKK03_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX83411.1"
                     /translation="MKRILISALLIVSMLLPALAQEKEQKLKELEQLEQQTKQVTIPD
                     TLVAAAEDTLIVAAEDTLAAIRAEGEEIAVVVSDDEGDTSTVRIGDIVTIKDTGDETV
                     IRIGKKGVRIFEDGDSTEIDFDDDEYWNEEATGRPGKSFDGHLGGIEIGYNNFSTATW
                     LNNDVPMDGYLDLNTAKSTSFNIVSPPVSLGFTRHFGLVTALGINFNNYRFDGNNSIV
                     VDGEGIVSPFFPAEPVRYEKSKLATIYGILPVILEVQIPVSHGSTINLGAGVIGAVKL
                     GSHTKVVYYNDGKQKDKNHDDFNLNVLRYGVTARAGYEMVQVYGTCYLSPMFEKGKGP
                     ELYPFEIGIALTIND"
     gene            complement(33069..34022)
                     /locus_tag="PKK03_03105"
     CDS             complement(33069..34022)
                     /locus_tag="PKK03_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83412.1"
                     /translation="MAITEDMDFPRLTPPDDRMPSGRRLFRRPAELTDEQFDLMAAAW
                     AEGALSGEAVEELESVMTASTDRRDRAGSFRSIRLVPYNENWPGMKSAVRLSPALTAF
                     RRSVIPTLLAVAAMVIMIIYGPAGAKLKTINSRGAVNGPAMTVAEIQVYAPIIAVERI
                     PEPNILPVRPVQTPAAEENASQGHLAEGMEKPAEITGLVRVEPIALARGHATASSVAP
                     ELIPAIISVNIRDIQPDMTLQEEKNWMFRSISFLAGAVTGKEKKIDAYMIANGCITGI
                     NNVFGWDMELDRISNKSGEPVAVTFSSSLLSFTKPVNKTTP"
     gene            complement(34013..34498)
                     /locus_tag="PKK03_03110"
     CDS             complement(34013..34498)
                     /locus_tag="PKK03_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNX83413.1"
                     /translation="MTVKEYNNAVREFGDHVFRFILRSIKDSHRADDIVQDTYEKLWR
                     CVTEIEYGAVKSWLFSTAYNRMIDVIRKDSRLVDVEYYDDSTLYTEENGNDLNEVLHR
                     ALEKLPAVQRSVILLRDYEGYNYREIGEITGLSEAQVKTYIFRGRVALRNYLVKTSVW
                     Q"
     assembly_gap    34729..34828
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(34851..>36397)
                     /locus_tag="PKK03_03115"
     CDS             complement(34851..>36397)
                     /locus_tag="PKK03_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNX83414.1"
                     /translation="SKIEQEEKALIAEYEKMIAFAGSEGLAKYTRLHESVNSAGFKQK
                     RKEIESLTYKGSEEHAREAEFLGLQKAKDIVSYFKTEGNALLKIFLSLDGSARIKEIE
                     DLQLFVQSPEFKQKERMKPVTFRDTEEYGKWLEFKKLKGDREVKKNLNPEKVKRFTEL
                     QALVTSAAFLLKKNMKPVTFKDTPEYQKLQELKTKKSATDIVNFYKFRESKDYHNWVQ
                     VKDSARLKRYYELQEYLKTKEFHERKEYLLDKRRFEKSEMFRELKEYETLKNSPEIKW
                     YFSVKDSNKFDMLKNRELTFSDEFDGTAPDSTRWTNRYYPGDKLLHDSYSISTDLHSY
                     TPSDNVEVRHSVLRIITKPQKINGKAWDPARGFYTKDFNFTSGIVNTGASFRQKYGTF
                     TAKIKLTDPNVRNAFWLIGDRITPHVDVCRSGSGKVWFDLFTSPGSHYRKSIGSRYLS
                     DFYIYTLDWTPNAMVWKINGVEVMRQTTGVPQEPMFINLAGGVDKPIGGISSMEVDWV
                     RVYQPK"
BASE COUNT         8539 a         9331 c         9371 g         9056 t
ORIGIN      
        1 tatagcatag gtgccaagcg agagaaagcg gtttacatct ttcatttcct tctcagtgtt
       61 gaaaatatga caaattcaat taaagctgat tttgtctcag aatcgggata ctcttcaagt
      121 atacggattg cccggtcaga gtactccttc attgccgcct cggcatagtc gagtcccccg
      181 tgttttttca cgaacccgat tacctccctg atctccctgc tgcccttttt tttctttctg
      241 aggagcctta ttatatggcg gcgctcaatg ggtccggttt cgcggagagc atgaatgagt
      301 ggcagggtta tctttttttc cttgatgtca tttccctccc gcttgcctgt cagcccctca
      361 cccgtataat cgagcagatc gtcctttatc tggaaagcga tgccgatatt ttcccccatt
      421 tccttcatcc ttgcgatggc atccggatca tcggtgaccg agctggctcc gcaggcggtg
      481 catgctgaga tgagggctgc cgtcttcatc tttattatcc ggaaataatc ttcttccctg
      541 atatcaagct tgcgggtctt ttgcagctga acaagctcgc ccttcactat ctcacgcacc
      601 gcgtccgaaa caatgcccag catgttgaaa cgctgcttct ccacacttat cagcaggccc
      661 tgggccagga gatagtcacc aaccaggact gctatcttgg aattatacaa tacgtttatt
      721 gaagggagtc cgcgtctttc agaggcatca tcaaccacat cgtcatgtac aagtgtcgct
      781 gtatgaagca gttctatgaa ggtggccgca atgtatgtcg tctcattgac tttcccgttc
      841 agcctggcgg tcagaaagac cagcagcggc ctcatctgtt tgcctttgcg gcgcagaaaa
      901 ttatcaagga tgacgtgaaa gaagggtatg tcgctttgca gggtgtgtct gaagtaagat
      961 tcaaactcac gcatctctgc cgctaccggt ttctttatgt ctgaaaggct tgccatcctc
     1021 ggctgaagct atgattacca aaaataagac aaaatgcgta acaaattgtt ccggcaggga
     1081 ggtttgatga taaccagcct taaatatcgt tcaggatttc ttttctccca tgaatctctt
     1141 ctggaagaca aggatggcca gaaccccggt agtgatcgat gtgtcagcca ggttgaacac
     1201 gggtctgaag aaaatgagtg tctgtccggg cctgaagggc gaccagtcag gccagtgtga
     1261 gtcaattaca gggaagtaga gcatgtcaac caccctgcca tgcaggaatg atgaatatcc
     1321 gcctccttcg ggaaacagca ccgcgggtgt gtgccagctt tcgctgaaga tcatgccgta
     1381 aaacgcgctg tcaatgaggt tccctgctgc tccggcaata attgcgctga ccgaaaggat
     1441 caggccggtg tgggcattct tttttatgat gctgtcaaga taccaggata tgccgacaat
     1501 ggccaccatc ctgaagaccg acagggcaac ctttcctgtc ttgcctcccc actccatgcc
     1561 aaaagccatt ccgttgttct caagaaaatg aatcctgaac cagtttccca gcacaggtat
     1621 ttcatcgccg ataaccatat tggttttgac aatgatcttt acaatctgat caacaatcag
     1681 gatggaaaat ataagaagca gtgcctttgt ccggtttgac atcacgattt aatttggccg
     1741 caaaatagca aaaaaacggc aattacctca tttaaatgct gatagtgtgt catccggcta
     1801 ttctcccggt gaacgggatc agtaataaag gaatctcttg atcttcagtg ttgcggtctt
     1861 ctggaacggc tccggctggt aaaccaccag ctgaagctgg ctgtacctgt tcacctgtga
     1921 attgacatac cgctgcagtt cttcaaggta ttcattgatg atcagctctg cctggtcggc
     1981 gaaatccttc ttcatctggg catatttggc ctgcagttcc tcgatgttca ggtgaaccat
     2041 ggcaacaagc ttgccctttt tctgtacaac aagtgattca atgacatggc cgaaatcatt
     2101 gatgactgat tcgatctctt ccgggtaaat attctctccg cttgagccaa cgatcatatt
     2161 tttaagcctt ccccttatgc tcaggtagcc atgtttgtca accactccga gatcaccggt
     2221 cctgaaccat ccgtcaggag tcagcacttc ggcggtcatt gccggttcgc ggtagtagcc
     2281 tttcatcaca ttttcgcccc ttgcccatat ttcaccttcg ccggtgacag ggtcgggatt
     2341 tttgatcctg agtgtgacgc cctgcatggc gggtccggtg gagccatgcc tgcatgagcc
     2401 gacctttgtt ccggcaagca gcggcgaagt ctcggtaaga ccgtatccga tggcatacgg
     2461 gaatctggca tcaagcagaa actgttcaac cgtcggattg agttttgcgc cgccaacacc
     2521 aaagaacctc agtctgcctc cgaatgcctc atatattttc tttcctgcaa tcctgttcag
     2581 cagacggcgg aacagcggag tgccataaag gaatgccaca accttgttct tgtggaacgt
     2641 aggggccacc ttgttgaaat agaccttctc catgatcagc ggcacggtaa gcatggttgt
     2701 gggcctgacc tgctgaaggg caggaagaag aactgatggc gtgggggcct tgcgcaggta
     2761 ccagatcatg gcaccgttga ataacggaag aatcaacccg agagtctgct cataggtatg
     2821 tgacagaggc aggattgaaa ggaaaacatc actctcaacc acatactgta ttgtcataac
     2881 ctgcctggcg gtaaagcaga tattcctgtg ggtcagcatt accccttttg acttgcctgt
     2941 tgtgcccgac gtatatataa tcacggccag gtcttcctct gctacattgt attttcctga
     3001 cggttcagac ccggcaacaa aaagtacctg gtcatttcct gaagtgatcc ggaaatcgtc
     3061 aattgcaatt accgtttcga gcgcttccgg tttgaattcg gcaaccttat gggcgaggtt
     3121 gcctgaaagg aacagggcct ttgcttcgga gtggttcagt acgttagcaa tctctcccgg
     3181 agtgaagtcc ggaagaattg gtacggctac cgctcccatg gaggttatgg ccaggtaagc
     3241 cgctccccag ttgggcatgc ttgtgctcag tatggccacc ttgtcaccgg gtgttatccg
     3301 gagatcttca aggagcctca tcactgaccg tgttctttcc ttgagctggt gatatgtaat
     3361 aacatcctct cccacaaatc ccattgcatt gttctttccg tgcttcctga agctctcccc
     3421 ggcaagaaac gggattgtca acatctcgtt attattcatt ggctatgaat gtattgggcg
     3481 caaagatact ttttaattaa tatgtacgac atttaaacgt gtaaatatca tagttaaata
     3541 tccccgtaaa caaatatcgg tctaaatagt ttatttttgc gtagaccaaa cacttgcaca
     3601 catggccctg atacgggtaa ccaaggaatt tcgctttgag atggcacatg ccctctggaa
     3661 ctatgacggt ccctgccgga acgttcatgg tcattcatat atcctctacg tgactcttgc
     3721 cggggagccg gtaaatgatc ctgaccatcc caggaacggg atggtgattg atttcggaga
     3781 tctgaagaag attgtccgtg agaacattgt tgacaggttt gaccatgccc tgatggtttc
     3841 cgggctggct cccgccgcca ctgttgaggc ataccgtcag cacttcggca atgtcatagt
     3901 ttcgccctac cagcctacgt gcgagaacct ggtagccgat attgcagcca cgctgggcgg
     3961 cagaatgccg gaaggaacat atctccacag catcaggtta tttgaaacag ccacggcctg
     4021 cgcggagtgg catgcatccg aaaacaggtg aaagagacag ataacgacag gcggctcttt
     4081 ctgctcgatg catatgcatt gatcttcaga tcatattatg ccttcataag aaatccccgt
     4141 gtcacatcca gggggatgaa tacatcagcc atcttcggct tcctgcttac gcttgaggat
     4201 ctgctctcca aacagaaacc ctcgcatatc gcagttgtct ttgacccgcc cggaccaacg
     4261 ttcaggaacg agatctatcc cgcctataag gcgaaccgtg acgccactcc tgaagacatc
     4321 aagattgccg tcccctatat caagcgtctc ctggaggcct tccgcattcc ggtcattgag
     4381 gttccgggct acgaggctga cgatgtgata gggacgctgg cacgcaaggc ggcagaacag
     4441 ggttacaggg cattcatgat gacccccgac aaggattttg cacagctggt cactgacaat
     4501 gtggtcatgt acaggccggg tcgctcggga ggtgaggcag agatatgggg cagggaagag
     4561 attgtccggg aatacggtgt gccaccggaa cacatcactg acctgctcgg gttgatgggt
     4621 gacacctccg acaatatccc cggggccccc ggaataggcc ctaaaacggc ctgtaagctc
     4681 attacggaac acggggcggt ggagagcctc cttgaaaaca cctcggcgct ccagggccgt
     4741 caaaaggaca ttatcgaaca aaacagggaa cagatacttc tttccaagcg ccttgccacc
     4801 atcgaacagg atgtgccggt tgagttccgg catgccgggt ttcaccggca ggagaccgac
     4861 acagaggcac tccggaagct ttacgaggag ctcgagttca gaaccatggc ctcaaagctt
     4921 cctgctcccg gtacccggca aaccgcccgg acagatctgc tgtctcagtc tgccggcgac
     4981 acagctgcac aggatgcgcc acctgctgcc gctatgccga agacaggtga gtcccaggga
     5041 tcactcttcg gtgaaatatc atcacctgcc ggaaaaacat cagcaaacat aggtactgct
     5101 cctcacagat ataccctgat cgagacggag aaggaatcgg caaggctggc ttcggagttg
     5161 tcaaaactga gcgatttctg ttttgacacc gagacaaact cgcttgatcc gctcaacgca
     5221 cagctggttt caatagcctt ctcctgggag aagggttcag gcacgatgct ctggctcccg
     5281 cctgagaagg acgaggccct gaaactcctt gaaccgctca ggtctgtcct ggagaatgaa
     5341 aagatcagga agaccgggca gaacctcaaa ttcgacatac aggtgcttgc gggatacgga
     5401 atcagcgtca gagggcccct ttttgacacc atgcttgccc actatcttct ggaacctgat
     5461 atgcgccaca atatggacct gctgtcagaa cagtaccttg gatatgaacc tgttcatatc
     5521 gaggagctta taggagaacg gggcccgcgg cagaaaacca tgagggaggt tgacagggag
     5581 agagtcaagg agtacgcagt tgaggatacc gatgtgacat ggcaactgat gaagctcttt
     5641 gagccgatgc tcataaaaca ggagctcgat acgctggcag ccgggattga aatgccgctg
     5701 atcagggtac tggcagccat ggaacgtacc ggggtgctgc tggatgaaag cgtactgaaa
     5761 aaatatgccg tcacccttcg ggagaatatc atcaggctgg agcaggagat ctacactttt
     5821 gccggacacg agttcaatat ctcatcgcct aaacaactgg gtgatatcct gtttgtcaga
     5881 ctgcgccttg acgacaatgc caggctgaca aagacaaagc agtaccgtac tgatgaagag
     5941 gtgctccagc gtcttacggg caggcacccc atcattgaaa aggtgcttga gtaccgcggg
     6001 ctgaagaaac tgctctccac ctatgtggag gcgctgcctc agcttgtaga tcccggtacc
     6061 ggcaggattc acaccacata caaccaggct gttgcctcta ccggcaggct cagctcaacc
     6121 aatcccaatc ttcagaacat cccggtacgg gattctgaag gccgtgaaat acgcaaggcc
     6181 tttattccgg cgaatggcaa gatcttcctg agtgcagact actcacagat agagctgagg
     6241 cttatggctc acctgagtgg tgacagcgcc atgatttccg attttctctc cgggcaggac
     6301 attcatgctg ccactgcctc gaagatcttc ggggtgccgg tagctgatgt cacacgtgag
     6361 atgcgcgcaa gggccaagac agccaacttc gggatcatct atggaatctc ggcctttggc
     6421 ctgtcagaga ggctgacaat aggccgcaag gaggcaaagg agctcattga cggctacttc
     6481 agctcatacc ccggtgttaa ggcatacatg gatgagagca tacgaagggc cagggagctc
     6541 gggtatgtca caacaatgtt cggcaggagg cgttacctgc ctgatattca ctcgaggaac
     6601 caggttgtgc gggggaacgc agaacggaac gccatcaacg cacctcttca gggcagcgca
     6661 gccgacataa tcaagattgc catgataaga atcgccacca ggctggagcg cgagatgccc
     6721 ggggcaaaga tgatcctgca ggtgcatgac gaactcatct tcgaggttga tgagagcgag
     6781 gtgaaaaagc tcgccgacct tgtcatggaa gagatgaagg gggcagcaca gctgagcgta
     6841 ccgctggagg tggatacggg aacgggttat aactggctgg aggcacactg aaaatggcag
     6901 cggaagtcag gattctgtat ttcggagcgg cacaggaagc caccgggaaa agcgatgaaa
     6961 caattgtggc gggggatacg gcttcagtgc gcaaccagct gatatccagg tatcctgccc
     7021 tgaagcagat ccctttcagg atggccctga accggacatt gcttaaaggt gaatctgagt
     7081 tacaaaaaag tgatattatt gcaataatgc ctcccttcca gggaggttga acattacact
     7141 tctgcaaatg aaaaacaggt atctggtcgg ggggccaatc acacctatcc ttatttctga
     7201 actgatgacg cttggcaatg atgatgccgg cgccggggca gtctcaatat tcatcggaag
     7261 ggtgcgtgac gatgagacgg acggaaagag agtaacggct atcgaatatt ctgcatacga
     7321 agaaatggca gtaaaggagg ctgaggaggt aatcacaacg gtcaaatcag ctttcagcga
     7381 tgtaaggtcg gtaatcattg ttcactccct gggagtggtg aaggcaggcg aggcttccct
     7441 ctttattatg gtcacgtctg gtcatcgcga tcacgcgaca cgcgcatgcc gccacgctct
     7501 tgagatgatc aaggaacggc tccccgtgtg gaaaaaggaa ctctactgag gaagggctcc
     7561 gtcaggggta ttcccggaga tgccattcag cccgcggctt ccgccggaac agcatgatat
     7621 ggtcatattc ctgccggagc cttaagcaac gatgcgtaat gcgccctgtt gccggcaatg
     7681 tcaatggctt tctttatctg ggtgtcacgg gtgactgaat attccaccat accccagtca
     7741 tagaaatacc tgcctgcaag ttccgattca atcagttccg tcacatccgg acggtacagt
     7801 gtcatctccc tctcgagggt atggctgagg cctgatttaa gtttctccat tgcctccctg
     7861 tttgaatcgt acagaccttc ctccatggct gttttggtca gctcctcaag gatactttca
     7921 gagtcggtgc ggtatgaaaa cttctttcct gcaaggaagt ttgtgaactg atccaggtca
     7981 ttttcagtga tcttcaggct gtcaagcacc ggaggccggg gatgagacca gtaatactct
     8041 gttgcaaagt cgaatatcat gttctgcaca aacagttcgc ctgcaaacct gttgagcatg
     8101 tcagagggca cggtgacatc ggggatgata ccgccaccgt ccctgacggg tctgccgctt
     8161 ttggttctga aggtctttgt gagcgaatcg ggaatagctc ccacactccc gtcctcattg
     8221 cggtgggaga agtcgaccgc ctggatgcat ctcccgcttg ggatgtaata cttggctgta
     8281 gtcatcttga cctgggcctt gtaactgaga ggacgggcca cctgtaccag accttttccg
     8341 taagaccgtt caccgactat gacaccgcgg tcaagatcct gaagcgctcc ggcaacaatc
     8401 tccgatgcag aggcagaacc cctgttgata agcacaacca ccggcatgtc cggtgcaatg
     8461 ggtgtcctgt ttgtacggaa taccgcatca tattgttttg ccctgcccct ggtgcttacc
     8521 acctcctggc ccggtttgac gaagagattg acaatctcga cagcttcatt gaccaaaccc
     8581 ccgggattgc ttctgagatc gaggattaaa tcttccgccc cctgtttatc cttgaggtca
     8641 atcagcgcct cccggacttc cgcgatgcag ttctgtgtga agttggtaaa ccggatatag
     8701 ccggtatttt cgtcaaccat tccgtaatag ggaacggcag agatggcaat tctttctctc
     8761 ctgagttttt tggtgatgtc ctggccgttt cgccttatga taatttccgc ctcggtgccc
     8821 ggttcgccct tgagcatgtc agaggccttg tctgaagaaa cccccttcag cgacgttccg
     8881 tcaatgctga tcatcaggtc gccgggtctg ataccgcttt tatcagcagg gaaaccctgg
     8941 tatacattgg ttacgaggat gtaatcacct gattttctga tcagagagcc aaaacctcca
     9001 tacttgccgg ttgtcatgaa atcaagatca ccggtctcct cctcagggta gtagacggtg
     9061 tacgggtcaa gggtagcaag catgctgtta atgctcctgg tcaccagctt ttcagggtca
     9121 atctcatcca cataaaaggc attcagttcc ctgaaaaggg agatgtatat atccagattt
     9181 ttggtgagcc tgaagtcctt gttttcctgc cctccaagga gaaatccgag ggtaatgacg
     9241 gcagtcacgg cgattaccgg aaccagcact atctttttta tgtttttgct attcattgct
     9301 gaaattttca caaagttaat atttaagtcc tgacactctg gaaatcgcct ctgttatgca
     9361 tttctccgta aattcacagg gtctgatcga ggtgtcggtc cagataatgg cgatgtcagc
     9421 atggcgtccg gcctgtttca tcagcattac aaggggctcc ttgtttttac gccaggcctc
     9481 cctgattctc cttcgcatga gattccggtc aacggctttc ttgaaatgcc ttttaggcac
     9541 cgtgatgagc actcttacgg ggtgaagccc ttcaatggca ggagtaaaac ggcagattac
     9601 cctcagtgac ggcagattca gggatctccc ttccgcaaag agctcgctga tggccctgac
     9661 tccgcagagt ctctcgcctt ttctgaatga tgcctcccgc tttttcatgg taaaaaagaa
     9721 agcatcaatc cgggtggatg gatgcttccg gttgtatttt atgttcctcc ggcgctattt
     9781 gttattcctg ttaaactctt taaggaagtt gtcaatggcc atggtcattg acggagcctt
     9841 gggattgggg gcctggagat caagcctgaa accctcgttg gttaccgcct cggcagtggt
     9901 ggggccgaag gctgcaatct taacctcgcc ctgcacaaat gtcgggaagt tttccttcag
     9961 ggacttgatc cccgaaggac tgaaaaacac tatcaggtca taatcaagct ccttggaatc
    10021 aaagtccctg cttatggtgc ggtacattgt gccgatggta tgcctgatcc tattccttgc
    10081 aagaagttcc ggtatatctg atttgtgggg atctgagagg ggaacgaaga agttttcctc
    10141 cttatgtttg attattacgt caatgaggtc ttcaaagttg gctttgccgt ggaatatctt
    10201 ccttttccgg taaactatgt acttctgcag gtagaacgcc acgttctcgg taatgcagaa
    10261 gtacttcatg gtgtcaggga cggttacccg caactcctca catatcctga aataatggtc
    10321 tattgcagtt ttcgaggtga aaatgattgc ggagaagtct gaaagattga tcttctgctg
    10381 gcggaaatcc ttcgcggaaa tgccttcaac ctggataaat tgtttgaaat caattttcag
    10441 gttatacttt tttgcgagct cgaaatatgg agatttcgga ttgtttggct caggctgcga
    10501 cactaatacc ttccttattt tcaaagcctt aagtttttag ttcaaccttc acattcatgc
    10561 cgcaggtcag tacacgccga gtagtttgag aatgaccatg acgggcagaa cttcaagggc
    10621 acaaaggtac aaaatgtaat aaaaaattga aacatgcctg ttatgaaaaa tgtaaagcaa
    10681 ccttaaggct ctggttaata agaatatagc agtgataatc aatcccgcaa ttatgaacgg
    10741 aagcgggttt tgaacgggtg cgaaaaggat taatccgtta agtatgaaga gaaaaaagga
    10801 gcaggcgaac catgaattgt atacaacgtt catgtactcg cggaacaggc ttttgagacc
    10861 gcttatcccg gcaagagcaa tgcatgacag gtgtctcagg agaagggcgg taaggaagga
    10921 gccggcaaaa atggcagtaa gtccgacggg tccgttgaaa agatcacggc cagcgaggcc
    10981 tgttaccagc agcgcggtgg atgcaaagag gctgacattc agtatggtaa aaatattcct
    11041 gagtacaggt ggccaggaaa acacctctga tgtgccaaca gggatggtct cttcatgcct
    11101 gcggaaactg attgatgaca atcccgtgat gatgcttctg cgtccgaata ccgttaggat
    11161 ggttatcagc agtagtgaga gcgagaggat aatgaagctc atgtcactgt ttaccggtcc
    11221 gggcggtctt ggcatgccgt caacgcgttt cagcccggag atataatctg ccgtaagcgc
    11281 ggtaacgctc ttttcctcca cggcaaaatc ttccgccggt gagatcaggg gaataatccg
    11341 tcctatgctg aactctgtct gccgcaccgg cagattgagg gtgtcagcgg caacactgat
    11401 ggtatcgccc ggaccgttgg tcatgagtgt atgttaaaag taagccggct tgccggggta
    11461 gagctctgaa agtatattat tggcaaggcg ggaggcacct atcctgaaat actccggtgt
    11521 aagccatttc gcaccaagca tgttttttac aatgaagtaa tagatgatgg cgtcatcggg
    11581 ggtgacaata ccgccggcag gcttgaatcc gatcattata ccggtctcgt tgtaataatc
    11641 gcgtatggcc ctgcagatca cccaagctgc ctcgggagtg gctgacacgc tgctcttgcc
    11701 cgttgatgtt ttaacaaagt cagcgcctga ctccatcgcg ataatggaag cccggtagat
    11761 gttcctttca tccttgagca acccagtctc caggatcacc ttcagggtgg tatctcctgc
    11821 agcttccctg atggccttca gttcatcgat gactgcgtca tagtcaccct tgaggaaagt
    11881 gcccacgggg atgaccacat caatctcgtc cgcacccatc ctgaccgcca tggaggtctc
    11941 aagtatcttc acctctgtga aagtctgggc ggtcgggaac gaggctgcca ccacggccag
    12001 tctgacgccg cacccgccaa gtccctcatg tgccacttta acaaagttgg ggtagacaca
    12061 cagagccgcc acgttgctga tgtcattgaa gtgataactg aactgggcgg ttctctctgc
    12121 aaagctcctg atggtggcgt cactgtcaga agtgttgagg ctggtgagat cgataaatga
    12181 aaatatttct ctcagaatcg gagccctttc tacagtgccg ggcgtccttt ttgcctcggc
    12241 aatgcattca cccatctcgc cgggcgagta gtcgttattt aagagttttt tgtataattt
    12301 gctcataatg agtttaatat gtgaacagca acagactgtc tgacggccgt caaacgcggg
    12361 tcaatttagt tgtttttagt gaaaagcaaa taaaaaacag actatttacg aaggctttta
    12421 tttcttgcca gcgaggcgaa tattcctgca aagcagagaa tggagaatac aatgaatccg
    12481 accttcatgg aattcatgag ctgcgggtaa ttgtccgggt ttattttttc ctgccccata
    12541 tagatggcca gcagcatcag tgctattgcc atactgaggg tctgccccac gtttctcatc
    12601 gtcccaagca tgcctgaagc attgccaagc tgtcttttct ctacggagct cattattgca
    12661 tttgagtttg gcgaagaaaa gagcgagaag ccggctccca tgattaccag tactgtcaca
    12721 atgaatgcca ccgaggtctg ctcagtcagg aagcagagca ttataagtcc ggctgaagtg
    12781 atacccatgc cggtcgaggc cagtacgccc ggattgtatt tgtcggagag tttcccggct
    12841 gcaggggaga aaagagccat ggtaaggggc catgacatca ggaccaggcc tgcttcgcgc
    12901 ggtccgagac ccctgatata ctgcaggaag aggctcagga agaagccgat tgcgcttgtg
    12961 gcagcataat gtatcagtgc cgcaagactt gagaatgcga agatcctgtt atgtgagatc
    13021 agcgtaacgt caaacaacgg atgaacagcc tttttctccc tcatgatgaa gagaggcata
    13081 agcaccacgc cggttgcagt tatcgcccat ccctctgcag agggcagtct ggagaatccg
    13141 tacataagcg ctgccatcga gatgccgtag attactgacc ccgcccagtc aaagctctct
    13201 ccttttgctt cagcccattc ccgtttcatc tttgtgcgga tgaggataag gcttattatt
    13261 cccatgggta tcaggaaggc aaagatgctc ctccatccca ggtacctggt aagcaggccg
    13321 ccaaggaacg gccccgaggc cagccctata tagactgcgg tcacgttgat tcccattgcc
    13381 cgtcccctct cccccggggg gaagactgat gtcagaatgg ccatgttggt tccgaacatc
    13441 atagccccgc cgataccctg cagaatcctg gtaccgatca gccaccggat gcccggggtg
    13501 aatgtcagca ggatcattga aagagtgaat accatcattc ctgcagtgaa caccttcctg
    13561 cgccccagta tgtcaccggc ccttcccgcc ggaagcagca acatcgaggt ggttagcatg
    13621 taactactta tgatccagtt taatgtaaca gcattaaggc tgagctcttc tccgatggag
    13681 ggaagcgcga gattgaccga cgagcccatg aacggggtca ggaatgatgc aaatgccgtg
    13741 accgacagca ctgaccgctt aagttccttt tcagtcattc cctgtttctg ctgtcaataa
    13801 tgattgtaac gggaccgtcg ttgacaagct caacatccat cattgcaccg aagacccctg
    13861 tggccggagt tctgccaaga aggccggctg tacgtaaaat gaactcttca taaagcggta
    13921 ttgccttttc cggtctggca gcacggatat atgaaggcct gtttcccttt ttggtcgagg
    13981 catgaagggt gaactggctg acaatcatta tcccgcttcc ggtttcaact gcagagagat
    14041 tcatcacccc gatgctgtca tcaaacagcc ggaggttcac cattttggcg gccatccagt
    14101 ttatatcctc agcagtatct gcatcttcaa accctgcaag cacaagcagg cccttcccta
    14161 ttgacgaatg taccttcccg tcaatggtca cggaagctct tttcactctc tggactacta
    14221 tcctcatacc ctgaaacagg gtggtaaaaa tagcaaatat tactattttt gcgtccatca
    14281 gtcagtcatg gacctgatta ctatcacagc aatagccctg ggcctctccc tcgacagctt
    14341 cgccgtctcg ctctcctgcg gagtgattga gtcaaagatc agattcagaa atgcggtgcg
    14401 gattgccttc attcttgctc tttttcaggg gggactgacg atagtgggtt ttttcctcgg
    14461 atcaacggtt tcagactaca tctcgaggta tgatcactgg gtggctttta ttctcctgat
    14521 ggttctcggg ataaggatga tcatcggcgg actgaaggtg acttccgacc ggcaacccat
    14581 agacatcaac cggttgccgg tggtgctgac aatggcagtg ggtacaagca ttgacgcctt
    14641 tgcggtaggt atcagcctgg catttctgaa ggttcagatc tggacggccg ggctgataat
    14701 aggaaccgta accttcctgg catccatgac tgcgatccgg atcggcaaat ctgccggctc
    14761 ccgccttggt tcaagggttg agataatcgg gggcattata ttggctgcaa tcggagtccg
    14821 cattcttgtt gagcatctcc tgtccgccgg ttgatccggc agacagtgtt gcgcgctgat
    14881 accctctctg acagaagaaa ggacggttaa agcggtatca tgaaccgttc cctgcctgaa
    14941 aaaacagcca tttccctgta cccggccctc ttcaaaagat catatgcttc gccgaaatac
    15001 tgtccgacac gttcaggaaa gtgagcgtca gagttgacgc acacagccac cccctgctgc
    15061 gcgaaaagat gcaggtattg tctgtcagga taaaaatcat gcagcggttt gttccggccg
    15121 ttggtattca gttcaaaggc aacctcatga atagcaaatg aagaagccat catgctgtaa
    15181 aggagggata tgtcagcctg cgggtaaaaa cggtgaatgc gcacgagatc aggatgcccc
    15241 atgatgtcaa agaatcccgt tgaggcagcc tcacagacca ggttgaaata gttctcatat
    15301 atctggtcaa catccttccc gatatagaat tcaggaccga ggtctacggt ttcatctccc
    15361 atataatgga ctgagccgat aatatagtcg aatggaaacg aattgataat tttttccgta
    15421 tccttttcgg tgccgggcag atagtcaatc tcgaggccga gccttacagc aatcaggtcc
    15481 ttatactccc tcttaagtct cagtatgtgt tcggcgtaag ccggaagcct gtcatgatcc
    15541 atgcaccatc ccgtgtctcc tcccagcggg ttcaggtggt cagaaaagcc cagttccttc
    15601 agtcccagtc ttatggctgc ctctatatat gcctcgggct cacgcttccc gtcactgtag
    15661 acggtatgca tatgataatc ggttccgtaa atcattcttc ctgctgtgat aaattgacag
    15721 ccctgattca aaaatataaa aggtgaacga ataatgtcgg ggattgttct aaatttgtag
    15781 gaataatggt tgttgaagca aataccccga aaaggaaatg caattgtctc tcctgtgagc
    15841 tgaggaatct tgtattcacc aaccttgaag aggaggatgt aaggcgtgtc tgtgaatcca
    15901 aggaagaatt ctcttatgcc aagggcgaga tcattcatag cgagggggat cccatacatt
    15961 acttcaaata ccttaaaacc ggccttgtca aactcttcag gctgacgccc gaaggtgacg
    16021 agcaggtgat taccattacc aggccttttg aatttgtaag caacaccaac gtcttccatg
    16081 aggataagta cagttactcc ctctctgccc ttgaagattc cgttgtgtgc tgcgtacgga
    16141 tagaattcat aaaggaactt atactccgga acgggaagtt tgcactgaac ctgatatctt
    16201 ctctttcccg cacaatggaa agcataatct cgctcggact ggagatcagg aagaggaacc
    16261 tggccggccg cacagccttt gtattgctct atttttcaaa cgaaatctat cactcgagga
    16321 tttttgagct tccggtctca cggaaggaga ttgccgacct catcagcatg agttccgcca
    16381 acgtgatacg taccttctcg gaattcaggc gtgacggcat tatcagggcc aacggccgta
    16441 ccatagagat cactgacatg aacaagctgg aagtgatttc acggcgaggg tgatcattgc
    16501 gccaaaataa ttatcttgat tacgtctaac cttaacctct gtttccgaca tgaaaaaaac
    16561 taccggagcc ttttttctgt tcctgtttgc ctcagtcatt taccccgtag gggcgcagcg
    16621 gcctgtcttt cccgactcat ctgtctatac tgttgagggg ccgcggcacg gtgtcagttt
    16681 ctttccccgg gtgcatcacc ctggagttga atacaccccg ggagatactc tctcttttga
    16741 caggtaccat acttacgggg tgatatgcca ctggatgaac cggtgggcgg aggatcatcc
    16801 ggaaattgtc accctttatg aggtgggccg cagctatgag gggagacctg tcatgcagat
    16861 gactatcacc agcggcaaaa acggttctga caccgacaaa cccgcggctt tctttgaggg
    16921 aaacaggcac agcggtgagg taaccggcgc agagtcagtc ctgtggctgg ccaggcacct
    16981 gatagaagga tatggcaatg acccgtcagt cactgacctg cttgaccatt tcaccgttta
    17041 cctcaggcct gtcaacaatc ctgacggcca tgagctgtac ctcaacactg cacagagcaa
    17101 tcgcagcacc gtacggcccg atgacaatga cggcgacggc ctctttgacg aggatgcacc
    17161 ggaggacctt gacggcgatg gcattatcct gacgcttcgc tggcctgaca ggaaaaacgg
    17221 cacattggtg cccgacccgg atgacccctc cggaagggtc atgaagagag taccccgcgg
    17281 cgagggtatc tatgcaacat cggccgaggg gattgataac gatggtgacg gcaggataaa
    17341 cgaagacggc attggcgggc ttgacctgca ccgcaactat cctgagaact ggaggcctga
    17401 aagggaacag acgggcaggg gtttcagcca gcgcggagca ggcgcatatc cgctcagcga
    17461 gcccgagacc agggcggttt ttaccttcct tatgactcac ccgaatattt atgtcgtcaa
    17521 ctccatggac accaccgtgc ctatgcacct gcgtcctccg tcaacatcgc catccgaaga
    17581 gagaatgtac cctgaagacc tgaagtggta ccgctatttt gatgacctgg ccaggaagat
    17641 aaccggttac gagaaagcgg gagacgtcta taatgactat ggcaacgggg aaccgctctt
    17701 cggccatggc cccgacttcg gatactggta ctatggtgca atctggtatg gagacgagct
    17761 ttggaacggc ggaaggccga aggattatga cggtgacggc gacaaagacc agcttgacat
    17821 gctggcatgg gatgaggagg aaaatagcgg cgagggtttc catgagtgga agcctgtgag
    17881 tcatccggtc catgacagtg tggaggccgg cggatttcat ccgaaatttt tcagccagaa
    17941 tccaccgcct gcacatcttg aaaaatgggc ttcaaaccag gcgctgttca ataaggagct
    18001 tataagccac ctgcccaggc tggaatggga ggagtactcg gttaaaagaa taaaaaaata
    18061 ccgcacagat tctgtcgatt ataagctgac catcaggttc aggaatgccg gcaggctccc
    18121 cacggccctc agccaggctc acctggtcaa gatggtcagg cctgatgagg taaaactgaa
    18181 atttgaaggt gacaccgccg gcataccccg cagcagggtt attgaaccgg cgtcaccggc
    18241 aaccggacgg ggaaacatga tgatgtccga agagcggagc cggaggcctg aatatgtaac
    18301 tgttgccgtg ggttataccc cgggaggggc caccgcggag gcaaacttca tcatcagggt
    18361 atacggagaa gccgaagtca ggggaacagc caccgttgaa accaccaggg ccggaatact
    18421 gccggagaaa gagttttttc tcggaaatcc gacttcacag gcaggccctc agggccgatg
    18481 aaatgtcagg atatctgaac cggtatccgg ctgatgacag atgatcaggc gagatccggc
    18541 tgcctgttgt cagtacaact gccatttcac ccagtacaac acgcaggagc caggcgggca
    18601 catgcgggag gaataccggc agccgcttct gcctcgccac ctcactcatc agctgatcat
    18661 gtgttacatg atgcggtgca acggcgttga aaggcccaga catggcggta tcagtaaggg
    18721 ctttgtgata aatgctgcaa aggtcggcta tatggatcca cgggaaatac tgttctccgg
    18781 gtgcaaacct tactaccagt ccggccctgg ccggtgctgt catccggttc agggctgatc
    18841 ctgagcgggc cagcaccact gcggtcctca tcctgaccac tctgacccct gcaccggaaa
    18901 agagatcggc tgccgcctcc cagagccggc atgtctcccc gagaaagtcc ccggcaggtg
    18961 ggtctgactc tgaaaagacc ctgtctgaat taactgatcc gtagtagttt accccggatg
    19021 cggttatgaa caccgacggg atttttccgg caccgacggt catcttatgg atgagctgcc
    19081 cggatacggt gcggctattg acaatatccc gcttacgggc ttctgtccac ctgccatcac
    19141 cgatatttgc agcggcaaga tggattatgg catcgccctc cctgaaggca tcggggtcac
    19201 agtagccgct atcggggtcc cacctgaatg actggtatct cccgttcttc tttcctcccc
    19261 gggtcaggtg caccaccagc catccctccg ctgcaaggag ggtgctcaga tatgatccga
    19321 tcagacctct gccgccagta attactaccc tgtgcatcta ctactcaagt gtttcaatta
    19381 tttcaatggc atcaccgatg catttctcgc acaccttctg cgagaggccc ttccgggcga
    19441 tgttgtcttt tccttcaggg gtgttgagat tctctccgag aagttctgag cactggtctg
    19501 ttccgttacg aacggtgaaa atcctgttga attcctttac tctgccgtat accgcggcct
    19561 tgtcgctttc ggaggggagg cctggcacac cgtgggccat gccgaaccag atgtatgcac
    19621 ctgtaaccgc cccgcatgtc ttctgcagtc ttcccattcc gccgccgaat gcggctgccg
    19681 cactggccgc atcacggctc ccgccggtac aggccatcag caccgcctgg gaacaactgt
    19741 agccctcacg aaagtaacct aaagccttat cttttttatt catttacttt tttgtcaaaa
    19801 atattaaaaa aacttaggag cttcagtttc cttttttgat atgattttga aaagtcctac
    19861 ttttaccgcc tgcattaaag tagttttaaa cacatcattt acaatggcaa accagaagaa
    19921 aatcaaagga gtgattggaa tcctgacggg aggaggtgac gttcccggtc tgaaccctgc
    19981 aatcagggcc gttaccatca gggccaacag ggaaggttat aaggtgatag ggctgcgcag
    20041 gggctggggc ggcatccttg agataatcag ggatccgaag gctgacaaca gccagtgttt
    20101 ccaggtcctt accgatgaca tggtgaacag ggcaggaagg acaggtggca cttttcttca
    20161 cacatcgcgc acaaggccga gccatgtggc aaagaactac gtccccgaac atcttaagga
    20221 tacctacaag gaagagatca atgacatgac cccggaggtg ctgaagaacc tcgaatggct
    20281 gggcattgac tacctgatcc ccatcggtgg tgatgacacc ctcagctatg gtgtgcatct
    20341 ctacaaacac ggagtgaagg ttgtggccat tcccaagacc atggacaatg acgttccggg
    20401 gactgactac tgcatcgggt tcagcacttg tgttacccgt acgatacaga tgaccaactc
    20461 acttcgcact tcggcagggt cacatgagcg tatcatggta ctggaggtgt tcggaaggta
    20521 tgccggtttc actgcaatgc tgcccaccat ggccggtgct gcaaacaggt gcgtcattcc
    20581 tgagtataaa tttgatatcg agcaacttac gaaactcctt gtagaagacc gcaactccaa
    20641 ccccagccgg tattcgatag tcctggtctc tgagggcgcc ctgtacaaag ggggtgacat
    20701 gatattccag gacagtgagc gtgatgccta cggacacgcc aagctgggcg gtatcggtga
    20761 ccttgtgtca gcagaaataa aggaacattc tgccaaattc aacaacggta aaaccataaa
    20821 tatcatcaac cagaagcttg gttacatggt caggggcggt gaccctgacg ctgttgactc
    20881 aatagtaccg atggcattcg gcaacctggc ccttgacctg ataatgaagg gtattcacgg
    20941 cagggtggtg gtactcaaga acggacgcta tgacaatgtg ccgattgacg tggtcacctc
    21001 ctccaagaag gttgtgaagg tttccgagta ttacaacgtc gaaaggctca ggccttacta
    21061 ctccaatttt gagatgaagc ccttcttcct tatggccgcc gagtattgat aacaactgac
    21121 aaatcagaca taaaaagagt gatgcccgtc aatccgtggc gtcacttttt tatttttgct
    21181 gcagaatcag gcgattgaag aagggtattg tcataaaatc gaccggaagc cgctaccggg
    21241 tggctgatga ggaggggaac atatatgagt gcctcctccg ggggcgattc agggtgagcg
    21301 gagcccgcac cacaaatccc gtcgccgttg gggatcatgt gacatttgac gctgatgagg
    21361 gggtcatcgt ggccctcatg cccaggcgga actatattat ccgcagggct tcgaaccttt
    21421 cgcgcgagtc gcagataata gccgccaaca ttgaccaggc attcctcgtc ataacagtga
    21481 agatgcctgc aacgccggtt gagttcattg acaggttcct cgccacggcg gaagcctatc
    21541 gcataccggc cgttatttta ataaacaaga ctgacctcta cggagaggag gagatggcac
    21601 gtgcggacct gattaaggat gtctacgaaa agatcggtta ccgcgtgatg atgatctcgg
    21661 ccactgagga caggagcctg gcggaggttg aagatgccct gcgaaaccgg ataaccctcc
    21721 tctccggcaa ttcgggggtg ggcaagagca ctctcctcaa caggctcaat ccggagcttt
    21781 ccctgaagac aggctccatt tccgattacc acgaacaggg taagcacata actacctttc
    21841 cggagatgca ctccgtcgcc gacggcggtt atgtcattga cacaccgggc ataagaggtt
    21901 ttggagtaat tgacttcgac cgtaaggaga tatatcactt tttcccggag atcttcagga
    21961 tctcttcagg gtgcaggttc tataactgcc tccaccttga cgaaccgggc tgcgcggtaa
    22021 gggaggcagt ggcacgcaat gacatagaac catggaggta cagaagctac ataagtatga
    22081 tgctggagga caatgaaaag tacagataag cgttatcctt acgggactta tcaccaatga
    22141 gaagaacttt cattgcagca tatattgctt ttgtactgtt gatgctgatc acggcactgt
    22201 tctatatcag cctctacagg aaccagattg aatactcgaa caagctgctt gacaggcagg
    22261 taatgattgc aggttctgac attgacaata caagcatgta tattgtcagc gacctgaacg
    22321 aaatcgattt ttccgatgac atcaccctct tcttttctga tcccggggtc aatgagcggg
    22381 cccgggaaaa gatgaaactg tactactcga agtacgggga aatcatcgtc ggtcttatgt
    22441 tatacaacac agatggtgat gtctataccc ttttcaagga cgaagagcgg aattcatggc
    22501 tcgacggatc atacagggct cagactgtgc caaccataca caccatggat aagcttgagc
    22561 ccgaccgtga caggtacagg tatttcctgc ctgttctcaa ggatggcgag gcgctgggta
    22621 acctggttat caccattgac ttccggcgtt atttctccgg catttttgcc aaatataacc
    22681 ttgaaaaata ccagtggcaa tgggtgatca atgacacagg aacggttgtg ttcgacaacg
    22741 gcggaaccaa tacatcctat gccggtgttg accgtatttc agagtcactt gcccggggca
    22801 attccgggag gctggcgcat acgatgagaa ccgatggagg agaggcagag gagatactct
    22861 catcatacta ccccgtaaac atcttcggac ttgatttttg ccttgtgttt tcggctcata
    22921 cggagttctt tcagaaatac attgtcagga ataccgtaat tctggggctg ctgaccatgc
    22981 tcaccctgct cttcatcata ttccttttcg gaaggttttt aaggaaacag gcaaaggccc
    23041 ttgagacgaa aaccgcctcg gaagccggcc tgctttccat ccttgaccag atgcccgttg
    23101 ggtttatcat ttataacgag ggaagagaga tccttcgtgc caacaggcag gcggccggcc
    23161 tgtttgcttt tgaggatgag acagcgatgg ccggtaagct catgcctgac ctctctagga
    23221 atgaatatgc catggacatt gccggtgagc tggggcaggg gaaggtgatc aggcttcccg
    23281 ccccgggcgg agaaaagata attttcctca acagcatacc tgttccatac aagggaggaa
    23341 acagcaccct ggatgcactc attgacatca cctcgcttga gctggcgcgc cgacaggagg
    23401 ctgacgcaaa tatcgccaag tcagagctgc tggcacgaat gagtttcgag atacgcactc
    23461 cgctcaacgg catactggga atgacggaga tgctcaaccg gtcagacctt ccggaagaat
    23521 caaaggaggt ggcaagactg ctcaggcgat cagctgacct gcttctcacc attatcaatg
    23581 atatctttga cgtatcgaaa gttgagacag gaaagatgat tcttgacgag atccccttca
    23641 ggctgcgtga tgaggtggct tactgtatca acctgatcag gcgccagaac ccggagattc
    23701 ctgtcaggtt tgaatcagag atagaacagt cagtgcctga aaacatcata ggtgatccat
    23761 acaggctgag gcaggttatc actaacctca tgaccaactc ggtcgccggc acacagtccg
    23821 gcctgatacg gctcagctgc cgtgtcagaa agtcggatgg caacagcttc acgcttgaat
    23881 tcaccctcac tgacacgggc aatacctata ccagggccga gataaagagg ttgttcggtg
    23941 attacatcac aaccatcagc gagcggtctg actggaccga ggacctgaag ctgggtccgg
    24001 tgcttgccag gcagctcacc gagctgatgg gcggcgaact tacggcagag agccctgcga
    24061 tgaaggatac ctcggggcag gagaagggcc tcagggttac cttcaccatc aatgttcatc
    24121 tcaatgaaaa gatatccaag aatatagagc ttggaatgta cgctgacccc ggcgacatac
    24181 gcacactggt gattaccggc ccgcatggac gagatgacga cttcctgagc atcatgcatc
    24241 gcgtgggact gcctgtctcg gtgaccagct tccagaagca tacagcttca cagatcaggg
    24301 cgggactgaa aagcggtacg ggaagaaaca ttttgcttgt actgttcgat gagccggaca
    24361 atgacggttt tgaggcagca aaggtactta tggatgaggg ccttacctcc gaacatattg
    24421 ttctgatgtt tacctcaagg gatccgaagg gacattacgc caggtgcgtt gacctgggta
    24481 tagaccatct tctggtcaag ccgtttgccc cggaggatct gatgagcgtc ctcagggagc
    24541 acttcccggc gctgaaggaa ctggcggcca ggttaaattc cgctaaaact gatgtccctg
    24601 tggtacttgt cgttgatgac aattacctta acaggaaggt tgtgggctcc ctgctcaggg
    24661 tactgggggt gggcgccgag tttgcatcgg gcggtgccga agctgttgat atggccgggg
    24721 agaaatcata tgacatcatc ctgatggacc ttataatgcc tgagacagac gggttcgagg
    24781 cctcgcggat cattctcggt aaaaatccgt ccactcagat agtggccctt tccgcggata
    24841 atatgcccga aaccaggctg aaagtcgaac agagcggcat gaaggagctc cttgccaagc
    24901 ctgtcactgt tgaggagctc aggagggtga tagacaggta ccacaagccc tcataacaat
    24961 ggcattctcc ccgaataaac tgccggctga tgaattcctg caactcaggg cctccctgcc
    25021 ggttgcagac gtcagatcac cctgtgaata tgccgtggga catgtccccg gtgcagtaaa
    25081 cattccactc ttcgatgatt cccagagggc agaggtggga acaatttaca agcgggaggg
    25141 caacgccacg gctgtgatta ggggcattga ccttgcagca cctcagatgt caggcaaact
    25201 gaaatcaggg ctggaacttg ctgccggcgg aaggcttctg gtttactgct ggcggggtgg
    25261 tatgaggtca gaggcaatgg catggctctt ttccctggga ggaatcgaac ccctggtgct
    25321 caggggaggt tacaaggcat tcaggaatca tatccttggt gatctgggaa gggaaaggaa
    25381 gtatgttatc ctcggggggc ttaccggaag cggcaagacc gggatactga gatatatgaa
    25441 gtctgccgga gagcaggtta ccgatctcga gggtcttgca tcacacaggg gctctgcttt
    25501 tggcgcactc gggcaggcgc cgcagcctgc ctcggaacac tttgccaacc tgctttatga
    25561 tgacctcagc gcacaccggg cggaatcgcc ggtatggctt gaggatgaga gccggaatat
    25621 cggaacggta ttcatgcccg actgtttctt tgaacggatg caatcagctc ctgtgattgc
    25681 cctgatgatg agcatcgaaa cacgtatgcc cagactggtc agggaatata cctcattccc
    25741 ggttgaggag ataatggctt cagtgatgaa aatctcaaaa cggctgggag gagaccggac
    25801 ccgtgaagcc cttgacgccc ttaaatgcgg ggactacccc acggccatca gaatcactct
    25861 tgagtattat gataagtcat atcattacgg gctgtcaaag agaccccggg atcaggttat
    25921 ctacattaac actgaaactg atgacgaggc tgtgaacgcc gccctggcaa tggaggctgc
    25981 ggaagggctg acgtgagttc acccgttttt cagagacgag gcgtcaaccc acctgaaaac
    26041 cttgtctgac cgcctcacaa ttccatctgt tttcaccgag cacagttctc ccttcaggac
    26101 tgtttccgct tcattctccg aagcatctct cagttctttt actgtaatct ccactattcc
    26161 ggttgacgga ccggtgatta ccagctcgtc accggcggag aggtctccgg tctcaagctt
    26221 tatctcggca actcccgctt ttctaaaata gttggtcacc ttcccgaggt agatttttct
    26281 ttttgtggct gacgagccgt aggaagtgct ccattccccg agtttccggc ccaggtagta
    26341 gccgtcccag aatcccctgt tgaataccgt ctcaagctgc ctcatccact cctcaacctt
    26401 ttcctgtccg aaggttccgt cagccactgc agctgccgcc tcgctgtagc accgggtgac
    26461 tgtcttaaca tattccggag gccgggcccg gccctcgatc ttgagcactc ttgccccggc
    26521 gtcaatgatc tggtcaagga aactgattgt gcagaggtct ttgggtgaca tgatatactg
    26581 gttatcaatg tcgagttcat agcctgtctc cctgtcgctg acggtatatc cgcgcctgca
    26641 cgtctggtag cagttgcccc tgttggctga ggcattgtac tcgtgcaggc tgagataaca
    26701 tttcccggag attgccatgc agagcgcacc gtgcacgaac atttccagct tcattggtcc
    26761 gcctcccggg ccacgtatat catcgcttat gatggtatca tgtattttct ttacctgctg
    26821 aaggttaagc tcccttgaga ggacagccac atcagcccac tgtgaataaa acttcagtga
    26881 tccggcgttg gaaatattga gctgggttga gagatggact tcaattcccg cctccctggc
    26941 ggcaaggatt gcggcctggt cggtggctat gactgctgtt atgccggcag cagcagcgga
    27001 agccagcacc tctttcatct cactcacctc ctcgtcatag acaacgatgt tcagcgtgag
    27061 gtagctcctg aggccgtgtt ccctgcagag tgcaactatg ccgggcagct cggcactggt
    27121 gaagttatgc gatgagcggg ccctcatgtt aagcttctcc acaccgaaat aaaccgagtc
    27181 agcccctccc tgtatcgcag ctctcagtgc ctcccgcgag ccggccgggg ccattacctc
    27241 tatgtcgttt cgcctgatca tttctgtcct taatcagccc tgttttttca gcattatcct
    27301 catacagcag ccgtggcccg gctcggagcg gagcacgaaa atctttccct tatggtattc
    27361 ttcgacgatc cttttggaga gggagaggcc gaggccccat ccccgttccc tggtggtgta
    27421 accgggttta aaaatagtct tgaaatcctt ccttggaatc cccttaccgg tatcctcgac
    27481 gtcaataaag gcttcggcgt tgttctcgct cagcctgacg gtgatctggc ctgtgccctt
    27541 catggcatcg gcggagttct ttaccaggtt ctcgatgacc cacccgagta gtacggcgtt
    27601 gaggggtact gccgtgcatc cggtcgtctc atcagccact gtcaggtcaa tggttgaggc
    27661 aattcttttc ctcaggtaag atgctgtggc cccggcaagt gctggcaggt tgtccggcac
    27721 cagcttcggt tttgatccta tgagggaaaa cctctccgca acgcggctca gcctgtcgac
    27781 gtcggcagga agctcctctg ctatatcggt ccccgggtac ctgctgctta ttatctcgct
    27841 ccatgccgag agagaggagg tgggtgttcc cagctgatgc gccgtctctc ttgacagccc
    27901 cacccatacc tggttctgtt cagccatccg cgaagcgctg aaagcaacat aggccacccc
    27961 gataaacaga agtatcacgg ccagctgcac atacggatat accctcagct gacgcaggag
    28021 cgtgctctca cggtaataca gatggttgac aatgccgttg ggcagctcaa tggagatggg
    28081 gtcattgcgt tttgccattc gtttcagttc gcggaccatt gccagggtgt cagttgcaag
    28141 cgctgccggc aggtttctct gtgagatgat gctctcctct tcgtctgtga gtataaccgg
    28201 tactgtggtg ttatcttcta tgatgctgaa caggaattcg aaatcgtcag aagtaaccag
    28261 cctgcgcgtg gcctccgccc attgctccgc cttcttgcgc tcctcctgct tgagcctgtg
    28321 caccaggtca tgggtataga tgagtgaccc gaggccgatc accaccgcaa atatcagaag
    28381 caataccttc cagagaagtc ttttccggta gatgttcatg ggctatcttt tctttttgag
    28441 aaggatttta atcgggctct ccttaacctc ttccttctgt ggttcagttg cagcaggctg
    28501 ctcctttccc tcttcccagg ttatgctgaa tttcggcttg ctctctgccg gggttgtccg
    28561 ggttgtgtct gccgagtacc agccgtactc ctcgttgagt attcccttca gcgtctgctt
    28621 ctcggcagca atgtcattct ttatattgtc ctgtgcctgt ccgaagtcat aacccacact
    28681 ggttttgcca tttacgcatt ctatcttcag cggcactgtt gccttgccgc tgccgtctac
    28741 cttcacctgc ccgaatgaac tgacgttacg gttgcgctcc cttgccttcc ggctcattac
    28801 ctctgaaaga agtagccgga catgataaat gtaatcacct tcaaacccgt gtgttccgta
    28861 gacggtgaaa tttacagccg aagagtttat cagcattttc ggaatggaaa cggttccgtt
    28921 attaatgaaa atgtcatttt ccattttcga gaaactgata ttcttcagtt cgtcgagatc
    28981 aagatatgcc gaaaggcttt ccgccggggc gaagctgatc agctttccgt cactgatgac
    29041 aagatgtgcc tcggccacta tcgccttgcg gaagaacctg taattgccgt cgagggggga
    29101 cagcatggtc acattgcccg tcagcctccc gcggaggttg tcgctcacaa tgaagttctg
    29161 tccgaagtta ttgaaggagg taaatgcctg gttaatatca acatttttaa catcaaggct
    29221 ggccttggta atgaatccgc catcctgttg cctgccaagc atcagttctc cctcaagcac
    29281 cccttcaagt ccgctggcgt atacttcgtc aaaggttatc acataaggcc tgtatgttaa
    29341 tctgctgctg aagttggcgg ccctgaagtt attgaatatc agactgtcag cttttagaag
    29401 tatatttgca ctgacgtccg ccggataaat atcagccggt ttgccggtgc tgtcaggctg
    29461 cttacttttt gcaggcacca gcgcagcagt gaccagcctg tcaacatgaa catcaccgtt
    29521 tatatcaagg ttctgacctc cgccggccag ccacgggaag aagtttttca tggctgcatt
    29581 gacggtaaag cgctggtcca ggaaagtaaa ggaaaggtta tcggcaatca ggtcattttt
    29641 taccgacagc gagccgttga catctttgag ggcaagcccg cgcctggcca gcgttgcccc
    29701 gaactctctg aaggaaagtg agatatccgg gctcaggtac gcaagagccg tggccggaag
    29761 tatgctgtcg ggaagcgttc cggaaagcct tatactccct gaaagtgtac ctgtctggtc
    29821 tttaatgtat cccgatctga aaatcttgtc gaggtcgtca aagtcaaggt caccgtcaag
    29881 attaagggtt atatggggtc ttcggaggtt gttcatcata aatgagccct tcaccgatgc
    29941 ggagccgtac ctggcggtga gtttgtcaac ggtgaaccgg aatgtctcgg ctgagttgtg
    30001 gtcgccgttg gtgagcgccc cgctgaactc caggttattg accctgaagc ctgtggagga
    30061 gtggctcatc cttcctcctg cgagtccgta ggtgaggtct atgtgaggtt tgccggcatc
    30121 gccgtaaggg cctgtgatac tgcacttcag gtcaatgatg cctgaggggg aaaagttacc
    30181 ggtgacggtt ttccatctgt caggcagcct tgagattacc gatgcaatgc tgattttcct
    30241 gccttcaatg atgaggttga gggttgaaga tgagtagttg acgttcccgg tgatgtcgaa
    30301 cctcaggtcg gctatgctga gtgctccctt tgccactgaa agggagtttg ccgacttgcg
    30361 gagtttgatg cccgcctcca ccgggatgtc tcttattgtt gttccttcca ggtttatgga
    30421 atcaatccct gcgttaagag cagtgctgag gtatatgcct gttctgaaca gttccccgcc
    30481 gagggttgcc gcccctattc tccctgccat gcgcattcca gagctcatgt cattgtacac
    30541 tgccctgaag tcaaccgcgc tgatgttctt cagcctgaca ttcttgccat ctccattctt
    30601 ttcagagaag acctcgtaat tgatgtcgcc cctccggtca gtcagcaggt taacctcacc
    30661 gtttcttgca gtgatcgact taactgccac cgtgccggta agaagtgacg gcatcgaaac
    30721 cgtcagtgac agcgaggagg cgtagagaag tgtgtcatta tcctcatgcc tcagctgttt
    30781 cttgtcgtag aacggggagg gttcaaccag gagatcactg aacctgacgg taatattggg
    30841 gaatgattca aagattgtaa tgtgaaggtt gccatacgaa atcttcgtat tgacgctctc
    30901 attgaccttc ctgataaggg attctgtcag gtgctcctgc cgcagcaggg catataatcc
    30961 cggtaccagg atgagcagca gcagcaaccc ggcggcaatc agcgctattt tccggaacag
    31021 tctcatggca cctggcggcg cagatgatta tcttttgctt attttcagca ggaaggagag
    31081 gtcacttccg ggcttcacct cccggggttc acttccgtac ctgaagacac gcgcatttct
    31141 gggtccgata agcgacagat ttttaccttc caggaggaac attgttcctt cgcgaagccc
    31201 tgcaacatac aactctctgt ttacctcaat gaactcctgt atcctctgct cgcgggtttc
    31261 gccggcatga ccggcaggat tggcatcgag gtaatgggga ttgatctgaa acgggatcag
    31321 cccgaaagcc ctgaaggatg atggtgcggt gacgggcatg tcattggtgg tcattattgt
    31381 ggggcaggtc acattcgagc ctgcgctcca tccgatgtac ggtgtgcccg acaggacctt
    31441 cttcctgacc ggggcaatca gcttattgtc tctcaggcat ttcagcagca tccaagtatt
    31501 tcctccgcct gctacaatag ccggtgcatc catgattgcc ttaaccggat ccctgaaatg
    31561 atggatcgag accacgtcat gccctacctc gttgaaacgg ttccttacct tctgttcata
    31621 ctcgtcatat gaaaatgtca ctgcggcata tgggatgaac agggccttga ccttcctgct
    31681 ccccaggaaa tccctgatat tgtttttcgg gtaatcgaga tatgcctctc cggcattggt
    31741 tgaattgctg ataagaagta gtctcatttt tgtctcgtta ttactttttt aaggggcggt
    31801 atgctgatcc accgccctat gtatcaacgc tccaaactta cataaaatca cccgattttg
    31861 tacagccggc gaaaggagag cttccggttt aactaaataa caacagaaaa ggggacatct
    31921 gtccgatgcc cccctgattc agaagttcag ttgtatgctg ttagtcgttg atcgtcagcg
    31981 caatgcctat ctcgaacggg tagagttcgg gtccctttcc cttttcgaac ataggcgaca
    32041 gatagcaggt gccgtacacc tggaccatct cgtatcccgc cctggcggtc actccgtacc
    32101 tcagcacatt gaggttgaag tcgtcatgat tcttgtcttt ctgcttcccg tcgttgtaat
    32161 agacaacctt tgtatgcgag ccaagcttca cggctccgat cacgccggcg cccaggttga
    32221 ttgtgcttcc gtgcgatacc ggtatctgca cttcaagtat taccggcagg atcccgtaaa
    32281 tcgttgcaag cttgctcttt tcgtacctga ccggctctgc cgggaagaac ggggagacaa
    32341 ttccttctcc gtcaactaca attgagttgt tgccgtcaaa gcggtagttg ttaaagttga
    32401 tgccaagggc tgtgaccagt ccgaaatggc gggtaaaacc caggctgaca gggggagaaa
    32461 caatattaaa gctggttgat tttgcggtgt tgagatcaag gtagccatcc atgggcacat
    32521 cattgttcag ccaggttgca gtggagaaat tgttgtaacc gatttcgatg ccaccaagat
    32581 ggccgtcaaa gctctttccc ggcctgccgg tcgcttcttc gttccagtat tcatcatcat
    32641 caaagtcaat ttctgtgctg tcaccgtcct caaagatcct tacacccttt ttaccgatcc
    32701 ggataacggt ctcgtcaccc gtgtccttaa tggtcacaat atcgccgatc cttacggttg
    32761 atgtgtcgcc ttcatcgtcg ctgacaacca cggcgatctc ttcaccttct gccctgatcg
    32821 ctgccagtgt gtcctctgct gcgacgatca gcgtgtcttc cgcggctgca accagcgtat
    32881 ccggaattgt gacttgtttt gtctgctgct caagctgttc aagctccttc agcttctgct
    32941 ccttctcctg tgccagggcc gggaggagca ttgaaacgat aagtaatgct gagattaata
    33001 ttcttttcat ggctttaaat agtttgttgt ttaatgccgg gacgtctgag ggtgccgaaa
    33061 agttacactc agggggttgt tttgttcaca ggtttcgtga aagagagcag ggaagagctg
    33121 aaagtgacag ccacgggctc accgctttta ttgctgatac ggtcgagttc catatcccat
    33181 ccgaatacgt tgttgatgcc tgtgatacag ccgtttgcta tcatataggc gtcaattttt
    33241 ttctcttttc cggtcacggc gcctgccagg aaggaaatgc tgcggaacat ccagttcttc
    33301 tcttcctgca aagtcatgtc gggctgaata tccctgatat tgaccgagat gatggccggt
    33361 attaattcag gggccactga tgaggctgta gcatggcccc gggcaagagc aatgggctca
    33421 accctgacaa gtccggttat ctctgcgggc ttttccatac cttctgcgag atggccctgc
    33481 gacgcattct cttcagcagc cggtgtctgt accggccgta ccggcaagat attgggttcg
    33541 ggaatccttt caactgcaat tatcggggcg tacacctgaa tttctgccac tgtcattgca
    33601 ggtccgttga cggctcccct tgagtttatg gttttaagtt ttgccccggc cggaccgtat
    33661 atgatcatta taaccattgc tgccactgca agcagtgtcg gaatcacgga gcggcggaat
    33721 gccgtcagcg ccggtgacag cctgacagct gacttcattc cgggccagtt ctcattgtat
    33781 ggcacaaggc ggatgctgcg gaaacttccg gcacggtctc ttctgtcggt actggctgtc
    33841 atgacggatt ccagttcttc cacggcctct cctgagagag ccccttcagc ccatgctgca
    33901 gccatcaggt caaactgctc atcggtcagt tccgcaggcc tgcggaaaag cctgcggccc
    33961 gatggcatcc tgtcatcagg cggcgtcagt cgcgggaaat ccatatcttc cgttattgcc
    34021 ataccgatgt ctttacaaga tagtttctca gagccacacg tcccctgaaa atataggtct
    34081 tgacctgtgc ctcagataag ccggttattt caccaatctc cctgtagttg tatccttcgt
    34141 agtcgcgcag cagtattacc gaccgctgga ctgccggcag cttttccagt gccctgtgca
    34201 gcacctcgtt caggtcgttg ccattttctt ccgtgtagag tgtgctgtca tcgtaatatt
    34261 caacatccac aagcctggag tcttttctta tcacgtcaat catcctgttg taggctgtag
    34321 agaagagcca tgacttcacg gcgccgtact cgatctcagt gacgcagcgc cacagcttct
    34381 cgtacgtatc ctgcacgata tcgtcagccc tgtggctgtc ctttatgctg cggaggatga
    34441 aacggaagac gtggtctccg aactccctga cggcgttatt gtactccttt acggtcattc
    34501 aggtcaatgc actgacaccc taaagacggc cgaaacaccc cgaatgttac agatacctgc
    34561 ataaaaaatt ggtaacgccg ggtccgagac ccggcgcgcg gcaatatgta tggatatatg
    34621 catatgatgc agataacgcc gggtctgaga cccggcgcta cgtcatatta ttgcagatat
    34681 ctgcctggca ggcagttatt tgggttggta caccctgacc caatcaacnn nnnnnnnnnn
    34741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnca gatatctgcc tggcaggcag ttatttgggt
    34861 tggtacaccc tgacccaatc aacctccatg gaggagattc cgccgattgg tttgtcaacg
    34921 cctccggcca ggttgataaa catcggttcc tggggcacgc cggtggtctg gcgcatcacc
    34981 tcgacgccgt tgatcttcca taccatggcg tttggcgtcc aatcgagggt gtagatgtag
    35041 aaatcagaaa gataccttga tccgatgctc ttcctgtaat ggcttcccgg cgaggtgaaa
    35101 aggtcaaacc agacctttcc gctgcccgac ctgcagacgt caacatgcgg tgtgatgcgg
    35161 tcaccgatca gccagaatgc gttacggaca ttcggatcag tgagtttgat cttggctgtg
    35221 aaggtgccgt acttctgcct gaacgaggcg ccggtgttga caatgccgga cgtgaaattg
    35281 aagtcttttg tgtaaaaacc ccttgccggg tcccatgcct tgccgtttat cttttgcggc
    35341 ttggttataa tgcgaagcac gctgtgacgt acctctacat tatcagaggg ggtataacta
    35401 tgaagatcag tggatatgct gtagctgtcg tgcagcagct tatcgcccgg ataatacctg
    35461 tttgtccacc tggtgctgtc gggtgcagtg ccgtcgaact cgtcgctgaa ggtaagctcc
    35521 ctgtttttaa gcatgtcaaa cttgtttgaa tccttaacac tgaagtacca cttgatttcg
    35581 ggtgaattct tcagggtttc gtattcctta agttctctga acatctcgct cttttcgaaa
    35641 cggcgcttgt caagaagata ttccttcctt tcgtgaaatt ctttagtctt aagatattcc
    35701 tggagttcat aatacctctt cagccgtgcg gaatccttta cctggaccca attgtgataa
    35761 tcctttgact cacggaactt gtagaagtta acaatgtcag tggcactctt ctttgtttta
    35821 agttcctgga gcttctggta ttcgggagtg tctttgaaag tgaccggctt catgttcttt
    35881 ttcagcagaa aagccgcgga agtaaccaat gcctgcagtt cggtgaacct cttcaccttt
    35941 tcgggattca ggttcttttt cacctccctg tcgcccttca gtttcttgaa ttccaaccat
    36001 ttgccatact cttcggtatc cctgaaggta acgggcttca tcctttcctt ctgtttgaac
    36061 tcaggcgatt gcacaaagag ctgaaggtct tcgatctcct taatccttgc cgagccgtcg
    36121 agcgacagaa aaatcttcag gagggcgttt ccttcggtct taaaatagga aacaatgtct
    36181 tttgccttct gcaagcccag gaattctgcc tcccgggcgt gctcctcaga gcctttgtat
    36241 gtgagtgatt ctatctcctt tctcttctgt ttaaaaccgg ctgagttgac gctttcatga
    36301 agacgtgtgt attttgccag gccttccgag ccggcaaagg caatcatctt ttcatactcg
    36361 gcaatcagag ccttctcttc ctgttcaatt ttggagg
//
LOCUS       DAOPXU010000018        35647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_1086_length_35647_cov_12.781805, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000018 DAOPXU010000000
VERSION     DAOPXU010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 35647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35647
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1086_length_35647_cov_12.781805"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..1100
                     /locus_tag="PKK03_03120"
     CDS             222..1100
                     /locus_tag="PKK03_03120"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNX83415.1"
                     /translation="MAKNEKRKGKWTDRFRFAIFNDTTFEELWRIRLSKANALLAGAL
                     LLVITLALNTSLIAFTNLREFIPGYPDVEMRRNILMNAIMLDSLEHELEIRDKYFRDL
                     NDVISGRQPASSVTLRDSTRDYSNIEFRKSSEDSLFRARVEKEDKYSLSASAISPGIP
                     STGQVTSLANIHFFPPAKGIISSNFDTRTRHYATDIVTQPKAVVSSTLDGTVIMTGWT
                     METGFVIMVQHANNLVSVYKHNARLLKEMGDRVRAGEPISIVGDSGELYTSGPHLHFE
                     LWHNGEALDPAQYVYF"
     gene            1115..2281
                     /locus_tag="PKK03_03125"
     CDS             1115..2281
                     /locus_tag="PKK03_03125"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNX83416.1"
                     /translation="MHKRLAIIGSTGSIGTQTLGIVARYPDLFTVKVLTAGSNAELLM
                     AQARLCRPEKVVIGNEAFYEVVKTGLSGLGIEVMAGQKAVEEVASSDGIDTVVAAMVG
                     FAGLRPTAAAVAAGREVALANKETLVVAGEMITRTAAITGTRLLPVDSEHSAIMQCLN
                     GEPESSVERIILTASGGPFRDTPAGRLAKVTPLEALRHPNWTMGSKITIDSATLMNKG
                     LEVIEAHWLFGTPPDRIDVLIHPQSIIHSMVQFTDGSLKAQLGVPDMRLPIIYALTWP
                     ERVVTELPRPDLAETGSLTFGEPDTARFRCLALARAALKEGGNMPCALNAANEVAVAA
                     FLREQIGFNDIAGTVEFVLAKTSFNSEASLSTYAETDNLSRRLADDYINKVLKK"
     gene            2278..3603
                     /gene="rseP"
                     /locus_tag="PKK03_03130"
     CDS             2278..3603
                     /gene="rseP"
                     /locus_tag="PKK03_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNX83417.1"
                     /translation="MTVLIKILQLLLSLSILVIVHEFGHFLFARIFKTRVEKFYLFFD
                     WPWAIFKKKFGETEYGIGMIPLGGYVKISGMIDESMDREQMKLPPQPYEFRSKKAWQR
                     LMIMLGGVFFNFIFALLIYVGVLSLWGETYLSAKNVRYGILTDSTGYAMGLRNGDKII
                     SVDGKEVDNFYAIIPDILLNERQVLTVDRSGEMVDIPIRREFIPILLKNPDIIDARIP
                     FGPFVVSGVAGESPAAAAGLAPGDEIVAIDGNPFIWYDEFQTYLAQHKARPVTVSVKR
                     GGELKDISVTPTGAGILGVFRETANILELSTKHYNFFEAVPAGISKGWKTTGDYLKQF
                     KLIFAKDTKGYESLGGFITIGSIFPGKWNWQSFWNLTAFLSLILAVMNILPIPALDGG
                     HVMFLIFEVVTGRKPSDKFMEYAQITGMVILLALLIFANGNDILKLIRK"
     gene            3672..3845
                     /gene="tatA"
                     /locus_tag="PKK03_03135"
     CDS             3672..3845
                     /gene="tatA"
                     /locus_tag="PKK03_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNX83418.1"
                     /translation="MGKIGPLEIILILLVVVLMFGGRKIPELMKGIGQGLKEFKKAKD
                     TGDDADKGTAKEN"
     gene            3911..4897
                     /locus_tag="PKK03_03140"
     CDS             3911..4897
                     /locus_tag="PKK03_03140"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HNX83419.1"
                     /translation="MSKASVFLFLMVAFIASCGDSSLTRTLPNVTGTTGEVLVVMDKY
                     MWDGSPGRAIREQVSPLLIGLPQPEPAFTILPVNPSGFRDIYVSHRNIIQVTIDPGIQ
                     KPVVTYTRNKWAETQLVVSIAAADTASLAAVVREKGDAIRRSINDAERERLTAWLTNS
                     AGKEKSILNAGEARWELAMPAGWKPDFNREGIMMISLETPSTTQTVMVSVADRTTARI
                     GCLELADLTQKRMSDEVKGPDGGSFMVIEQKIPVSCRSFRRYDTDYIEMRGLWTLEGG
                     FMGGPFISYAFIDPATTRAVVVTGFVYAPKEEKRELLRQVEALMYAVKKRQE"
     gene            complement(5034..6125)
                     /gene="mqnC"
                     /locus_tag="PKK03_03145"
     CDS             complement(5034..6125)
                     /gene="mqnC"
                     /locus_tag="PKK03_03145"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HNX83420.1"
                     /translation="MGIREQIYDRLAGGGRLTVDEALSLWNEAPPEELMYVADLTRQR
                     LHPGNRVGWIIDRNVNITNICFSQCRFCNFCRTEKHEDAYVTTDEEYDRKIRELLALG
                     GDQLLLQGGMNPALGLGFYTDLFRRLKSRWPGVKLHALGPPEVAWLARAEKMGYHDVL
                     VALRNAGLDSLPGAGAEILSDRVRKLISPVKATVAEWLGVMREAHRLNLPTSATMMFG
                     HAETVLERLEHLDMLRKLQDARPAGHDGFITFIPWPFMDRGTRLAEKDGIYSTVTAPE
                     YLKLIAISRLMLDNIPNIQASILTVGKETAMISLHCGANDLGSVMIEENVVSAAGDPF
                     RVSPGEMQQIIREAGFIPVRRDQRYVPVD"
     gene            6253..7359
                     /gene="pdxA"
                     /locus_tag="PKK03_03150"
     CDS             6253..7359
                     /gene="pdxA"
                     /locus_tag="PKK03_03150"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNX83421.1"
                     /translation="MDKKPIVAGITQGDMNGIGYEIIMKALSDPEIAELCVPVVYGSP
                     KVAAYHRKVLNITNFNFNNIRSVAEAHPKKVNLLNCLGDDVHVELGKSTPEGGQSSLA
                     ALEAAVSDLTAGRIDVLVTAPIDKSNIQSDSFRFTGHTDYLKSKTGSHDVLMLMISES
                     MKIGFVTEHMPLKDVPGAITIGNILTKLRLLNRSLLQDFAIRGPRIGVLSLNPHAGDN
                     GLLGHEEKDIIGPAITRANEEGILAFGPYSSDGFFGSGAFKRFDAVLAMYHDQGMGAF
                     KALAFDTGVNFTAGLQVIRTSPVHGTAFDITGKNEASGYSMRQAIYSAVDIFRNREMY
                     EEIKRNPLQPQKIEIHPEHVDELPPETENYDQAL"
     gene            7356..7937
                     /gene="ruvA"
                     /locus_tag="PKK03_03155"
     CDS             7356..7937
                     /gene="ruvA"
                     /locus_tag="PKK03_03155"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNX83422.1"
                     /translation="MIDYITGTVTELNPAFAVVECNGIGYGINISLNTFSALEKDTSC
                     RILIHEAIREDAHLLYGFATADERDLFRQLITVSGVGAGTARMMLSSVTPSDLREAII
                     SGNVAILKGVKGIGLKTAQRIIVDLKDKIGKHAVSGEILAFSDNTAREEALSALVMLG
                     FARSSVSKVIDNILRENKKLQVEEIVRKALKLL"
     gene            7990..15357
                     /gene="sprA"
                     /locus_tag="PKK03_03160"
     CDS             7990..15357
                     /gene="sprA"
                     /locus_tag="PKK03_03160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNX83423.1"
                     /translation="MGLLILLSLSSFVIKAQTNPGDSLLRLNRDPAFPWEAKKSSPLF
                     LRNPSNITTRVEYDGRNNQYIIYQKAGALDFRKPVYMSAEEYRRFEFNQAMREYWDAS
                     LRGDATGYRSSLIPQIEVGGETFDRIFGSNIINIVPQGSAELIFGINISHTENPTLSE
                     RLRTIPTFDFQEKIQMNVTGTIGDKMQLGINYNTEAMFEFENRTKLEYSGKEDEIIKK
                     IEAGDVTLPLNGTLITGSYSLFGLKTELQFGKLTMTTVFSQQKGESSVVEVKGGSQIS
                     DYEITADNYEANRHFFLSQEFRENFDGALSNLPIVSTGLNIEKIEVWITNQTSRFEDV
                     SNRNIIAFLDLAENQANIYNTVPAFQGTPGAPVNPSNNTNGLYEQLNTTYGNVRNIDQ
                     VADAFAGLYPGFQIGRDYEKIENARKLTEREYTVNRQLGYISLNVALNNDEVLAVAYE
                     YTLNGQVFKVGEFSTDGITAPDALILKLIKGTALSPQIPTWRLMMKNIYNIGSGRIEP
                     KNFEVNILYQDDKTGNALNYLPGTPLDGQVLLQALGLDQLNSQLDRQPDGLFDFVDGI
                     TVMAEKGRIIFPVLEPFGRHLLKYITDPEQIRKYVFTELYDSTQTVARQMAEKNKYVL
                     SGQFTSASGSEIRLNVANIPAGSVKVTAGGVTLTENVDYTVDYNMGSVKIINSAIIES
                     QTPVQVSLESNQFFGLQTKTLVGTHLNYRLSDRFNLGGTVLRLTERPYTQKVTYGDDP
                     ISNTIYGFDASYRSESQLLTNAIDWLPFIETNAPSSINFFGEFAHLIPGHSRAITSEG
                     AVYIDDFEASEISLDLRAFNAWSLASVPQGLDHFFPEARLSKDIRSGFNRARMAWYVI
                     DPLFLRNGSTTPGHIRSNPDTQSSHFVREIYETEIFPYKESPSGIPTNISVLNVAFYP
                     DERGPYNFDVLPGAYSAGLSSDGLLNSPRTRWGGMMRELLTNDFESANIQYIKFWVMD
                     PFVENPDHEGGDLYFNLGNISEDILRDSRKQFENGLPTSPLVLNVDTTVWGRVPTVQA
                     VVHAFDNNIESRRYQDVGLDGLGNQDEISFFDSYLQAVGAVVSPEVLDKIFEDPANDD
                     FHYFRGSDYDLLQLGILERYKKFNGTESNSPTSEMSNESYPTSGSTLPDMEDINRDNT
                     LSETESYYQYHVSLRPADMVVGRNFIVDELEYNATFANGEKSPVKWYQFKIPITDYER
                     IVGSIRDFKSIRFLRMYMRNFDEQVIMRFARLELVRSEWRKYNLTFFEGGERITVPEE
                     DDGTFEISSVSIEENAGKEPVNYVLPPGFDRVIDPANPQLRQLNEQSMVLRVRELSDG
                     DARATYKNVTLDMRQYRRLKMEVHAEALIGEPLHDDEVTVFVRLGSDYRSNFYEYEIP
                     VKLTPYGRYNNDSEEQRAVVWPELNKIDIDLSQLLDAKQSRDAAMRVPGSAVSEADIW
                     SVQDGRARISVSGSPNLSNVRVVMVGVRNPIRTRRPQTDDGAPRSAEVWINELRLSDF
                     REDGGWAANAQMQARLADLGTINLVGQTSTPGWGSIDKNVNQRSMEQVIQYDLSSNLE
                     LGKFFPEKAGIRIPVYMGYSENRIRPQYDPLDPDILLNDALDNLESKEERDSVLNLSE
                     EYSRRRTLTVSNAGTTKRGEKPHAWDPANVTVSYAFNEVYNTDPRTEVDVERTYRGGI
                     AYDFEARPKNFTPFQKSSLFSSSAFRLIKDFNIYLFPKHITVRTDLYRYYNEVKTRNI
                     NNPNLLIEPVFTKDFQWNRFYDVKYDITKQLKVDFTASSIARIDEPAGGVDRRRYPEL
                     FDAWRDSIMTNLADFGRTTSYYHLLNLNYNIPVNKLPLLSWVTSNARYSARYDWLAGT
                     IFPDSLNINPGNTIKNSNNGQFTLQANLTNLYSKVKFLKEVEASTQPGAVRRMSLGYE
                     EVTHSRRGLNFKAGRPRIINHNLKTKDVTVTVTAPDGTVVSGKFDVVSDVRIDFTADA
                     DVTNASVDIKGRVEKKPGIASVAGKYVVRGLMALRNVSATYTVEQGHILPGYLPGTTL
                     LGQQNYGGMSSPGWRFLVGLTDERFFDEAVVNGWITTDTLLNNAAAFSKREQINMRAN
                     VEPFPGLRIDITGDRRYSETVSSYLRADRNGNFPDSTRNTRITGNFTISVVSWGTAFE
                     KIPKSGDYVSGTFERFRGYTTLISQRRADERLRVDNSYDPGFDPVTGEPVTGPYASGY
                     GLTSAEVLVPAFLAAYTKRDPDKISLSPFPSMLRMMPNWRINFEGLTKFEAVRKVFNS
                     VSLSHQYRSTYTIGSFNTSLYYDPDETGISRIRDLKSNFIPQYEINTVTINEQFSPFL
                     NIDLGWKNSLTTRIEYRKSRTVTLNLTSNQVADIRNDEITIGAGYRFDDVAITLRSRT
                     GQRALESDLNIKLDFSIRDNKTLARKLIEEVNQPVAGQRVFTLGATADYVLSDRFNLQ
                     IYADHTMNDPFVANTFLTSNTNFGFSLRFTLVQ"
     gene            15470..15850
                     /gene="gcvH"
                     /locus_tag="PKK03_03165"
     CDS             15470..15850
                     /gene="gcvH"
                     /locus_tag="PKK03_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNX83424.1"
                     /translation="MNVPTNLKYTKDHEWVLIKGNEATVGITDFAQHELGDIVFIEIE
                     TVGEELGHGEVFGTVEAVKTVSDLFMPVSGTVKEKNEELDTTAELVNSDPYGKGWMIR
                     VELSDPSEIDALLDAEAYKKLIGA"
     gene            complement(15930..18848)
                     /gene="infB"
                     /locus_tag="PKK03_03170"
     CDS             complement(15930..18848)
                     /gene="infB"
                     /locus_tag="PKK03_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNX83425.1"
                     /translation="MTEDKKAIRLSKAAREFNVGIQTIVEFLHKKGFDISHDPNGKIP
                     PEAYALIVKEYSSDLSVKKDAEKLALKEKITPKKSLSIEDMESGREGDNGEPDEDFLI
                     TDISGVKKHIDLRTEIRKPEIRTVGKVDLTPPVRPVQKVPEAPVEKAPAAAAHAPEPA
                     VTTTTEAPVTAETPEIPAEPRQPKVVGRVDLDKLVKPEKPSKKAAGKKTEAAPEVKKQ
                     EPAAPRPEPEPVKAAPEPEPQVTQEPKETEIFRPGYEKLAGPKMVGKIELVPEVKKSR
                     DQSAADKHRHDKKRERIKPKPREKVALDTPVAPPQQEARKDKKGPQKDHRGGDRKKSF
                     RALRPEVDREDVDKNIRDVNLLMSKSKSKVAKYRRDKRDAISQRMKDETEKKELEKSV
                     LKVTEFVSVNEMATMMDVPVVDIISTCMSLGLIVSINQRLDAETMALVAEEFGFTVEF
                     VSAELQEAVKEEEDDETNLQPRPPIVTVMGHVDHGKTKLLDYIRNTNVVAGEAGGITQ
                     HIGAYTVTLEGEKTITFLDTPGHEAFTAMRARGAQITDIVIIVVAADDGVMPQTREAI
                     NHAHAAGVPIIFAINKIDKPTANPDRIREELANMNFLVEEWGGKYQSQEISAKSGINI
                     DLLLEKILLQAEMLELKANPDKLAIGTVLESSLDKGRGFVATMLVKAGTLNVGDVVIA
                     GSCFGHVKAMYNERNQKITEAGPSMPVLILGLNGAPQAGDKFNVMESDKEAKDIATKR
                     AQLQREQGLRTQKHITLDEIGRRIAIGNFQELNIIVKGDVDGSVEALSDSLIKLSTEE
                     IQVNIIHKAVGQISESDILLAAASNAVVVGFQVRPSLSARKLAEKEGIDIRLYSIIYD
                     AIEEIRAAMEGMLSPEIKEEIVATVEVREVFRIGKVGTVAGCFVRDGKITRNTRVRVI
                     RDGIVIYTGDLGSLKRFKDDVREVTSGYECGLNIDNFNDIKVGDVIEGYQTVQVKKTL
                     "
     gene            complement(18907..20151)
                     /gene="nusA"
                     /locus_tag="PKK03_03175"
     CDS             complement(18907..20151)
                     /gene="nusA"
                     /locus_tag="PKK03_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNX83426.1"
                     /translation="MENVNLIDTFSEFKELKNIDRPTMMSVLEDVFRGMLAKRYGSAD
                     NFDIIVNIDKGDFEIWRNREVVDDEAFTDENTQIPLSEAHKIDEDYEVGEEVSDEVKF
                     LDFGRRAILALRQNLVARILDLEKNNIYLKYKDRIGDIVTGEVYQVWKRETLVLDDDG
                     NELILPKGEQIPSDHFRKGDTIRAVVIRVEMKNNTPNIILSRTSPVFLERLFELEVPE
                     IFDGLITIRKIVRVPGERAKVAVESYDERIDPVGACVGMKGSRIHGIVRELRNENIDV
                     INFTSNIQLFITRALNPAKISSIKLIESEKKAEIYLKPSEVSLAIGKGGLNIKLAGQL
                     TGYDIEVYREPDNEDEEDVNLDEFADEIEGWIIDELKAIGCDTARSVLRLTVHDLVKR
                     TDLEEETIKEVVNILKSEFEKE"
     gene            complement(20169..20618)
                     /locus_tag="PKK03_03180"
     CDS             complement(20169..20618)
                     /locus_tag="PKK03_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83427.1"
                     /translation="MITKDHIRKLAEAHISGTGIFLVDVRLSSTGRITVLIDRTEGVT
                     IDDCAMLSRQISNDLGEEVGDYELNVSSPGLDMPLLVLQQFMKNEGRMVDVVTNEGER
                     FKGTMMNVTTGGFDLKTEKKTGKEITETVRSFNFEDVKSVKVIISFK"
     gene            20822..22054
                     /locus_tag="PKK03_03185"
     CDS             20822..22054
                     /locus_tag="PKK03_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNX83428.1"
                     /translation="MTNRRKIINDPVYGFISIPNDLIYDLMGHQWFQRLRNIRQLGLS
                     SLVYPGAVHSRFQHSLGAMYLTGQAVHTLRSKGTEISAEEEEGVLAAILLHDIGHGPF
                     SHALEHSLIEDMPHETMSLLIMEELNRSMGGRLTSAIAIFRGEYQRRFFHDLVTGQMD
                     MDRMDYLRRDSFFTGVIEGSVGSDRIIRMLNVSDDRLVVDEKGIYSVEKFLIARRMMY
                     WQVYNHRTVVSAEKLLTSLLVRARQVTAAGLKLFATPALAYFLEPRHNRAEGEERASL
                     VGHFTALDESDILSATKVWMSCGDRVLEELCRRFVNRDLLAIELQRTPFDPARVSETG
                     RMAKEVLGLTDDEVGYFVNTGDVYNQTYAPGTPEVRILLKNGTTPDITAVSDLFDKEA
                     LSDKVTKYYLCYPKEIIK"
     gene            22068..23105
                     /gene="lpxD"
                     /locus_tag="PKK03_03190"
     CDS             22068..23105
                     /gene="lpxD"
                     /locus_tag="PKK03_03190"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNX83429.1"
                     /translation="MEFTAAMIAQILNGTVEGDPETKLNTVARIEEGGAGALSFLANP
                     KYEPYLYTTTSSAVLVRNDFAPAKEVKATLIRVDDPYQALARLLAFYEQARPAKKGIH
                     ASAVIDEKASVGADVFIGACAVISENVVIGDGCSLHPQVFIGENVKIGRNCMLHPGVK
                     VYRDCVIGNDCIIHAGAVIGADGFGFAPVTDANFMKIPQIGNVILEDNVEIGANTCVD
                     RATMGSTIIRKGVKLDNLIQVGHNCEIGENTVIAAQSGLAGSTIVGRNCMFAGQVGLA
                     GHLRIADGVKIGAQSGIMSTISEENATYLGYPATDSKQFLKTTVYLRKLPDLSNRVDA
                     LAKAVESLKNS"
     gene            23220..24611
                     /locus_tag="PKK03_03195"
     CDS             23220..24611
                     /locus_tag="PKK03_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HNX83430.1"
                     /translation="MEKQRTLANEVSLNGKGLHSGIDVIVTFKPAPPNHGYKFCRTDL
                     PGAPLIDAVADHVTETSRGTTLVQGDASVATIEHVLAAFAGMRVDNALIEINGPEAPI
                     MGGSSRMFVEAITKAGIIEQEEERNYYVIKEKIAFSDEEHGVDLIIYPDDHFSAHVLI
                     DYNSKILGNQYAILDQINDFEKDIAGSRTFVFFHELEPLFRMGLIKGGDLENAVVILE
                     REVEQEEIDRIAKMFNKPGITEHHAGVLNNTKLRYPNEPARHKLLDLIGDLALVGQPI
                     KGKVVATRPGHYSNTRLARLIRQEIKRQTTKKEIPLLDTTKPAVMDIQQIKKLLPHRF
                     PFLMVDRILELTTSHVIGMKNTTYNEAFFQGHFEQEHVFPGVLLVETMAQVGGILVLH
                     NVDEPERYSTYFLKIDRMKFKHKVIPGDTIIIRADMTEPIRRNIVIMYCQAFVGEKLV
                     AEGEMTAQVIKNK"
     gene            24618..25391
                     /gene="lpxA"
                     /locus_tag="PKK03_03200"
     CDS             24618..25391
                     /gene="lpxA"
                     /locus_tag="PKK03_03200"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNX83431.1"
                     /translation="MISSLAHIDPKAQLGKGVTVEPFATIYGDVVIGDGTWIGSGAVI
                     YDGARIGSNCRIFNGASISAIPQDLKFSGEETTLEIGDNTTVREFATLNRGTRARGRT
                     IIGKNCLLMAYVHVAHDCSLGDNVIMVASSGIAGEVELGNWVTLGGGTIVHQFVRIGN
                     HAFLGGGSKVRMDVPPFIKADREPLAYMGINSVGLERRGFTKEKMHELHEIYRAYFNM
                     GMNGTKALEYIGANFPSTPERDYILDFISKSARGVIRGR"
     gene            complement(26270..27877)
                     /locus_tag="PKK03_03205"
     CDS             complement(26270..27877)
                     /locus_tag="PKK03_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNX83432.1"
                     /translation="MKKYLFVAFFAIVLSSSTLEGQEKYIQVPVSHVTVFSRGAQLSG
                     DAAVTLQPGTWEYVAGGLSPYIDPNSIQVRGEGDFMIMGVTHRNNYLENPAESDEIST
                     LRDKIKALQIRIEDEETATEVLLEKERFLKANYDVVSQKSAITPDQLKALMDIYGTGM
                     ESVKTAILKKNRILKEYREEKEKLDQQLAGTIDRSKMPTGEIVMTLSGSKPVTGRLNI
                     SYVVMNAGWQPAYDIRVDDITDPAIIIYKANIWQNSGVEWKDVKVSLSNAAPMTAGYL
                     PVLDPWFIDFYQEYALNEMVVVGYGTKGRPARQAKAEDAVMMDLEASAPMPVTVTESN
                     ISFSFDINVPKTIASGGKQETVELQRLTVPATYSYAATPKIASSAYLMGYITEWDKYN
                     LLPGESNIYFSNTFTGKGYINTAELTDTLPVSLGADNSITVKRDRRTDFTSQKMIGAN
                     RVETLSFLISVRNNKSKAVTVRLRDQLPLPQNSDITVEAVELSGGKQHSATGEVTWDL
                     PVAPRETKEIVFTYSVKYPKNKKVILE"
     gene            complement(28002..28508)
                     /locus_tag="PKK03_03210"
     CDS             complement(28002..28508)
                     /locus_tag="PKK03_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNX83433.1"
                     /translation="MTAQEFTSSIIDLKGNLQKYALSLTLDRDNALDLVQDTFLKAIQ
                     NQDKFVDATNLKAWVFTIMKNTFINNYRRKMRENTMMDGTQELYYLNLPSDKGSISPE
                     SSYAGNEIEKAIEELHEDYRLPFRMHVDGFKYEEIAENLNLKLGTVKSRIFFARQKLM
                     SVLKDYNG"
     gene            28867..29679
                     /locus_tag="PKK03_03215"
     CDS             28867..29679
                     /locus_tag="PKK03_03215"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNX83434.1"
                     /translation="MELRNLPSGKTAFYRFEAAGEPRSVILLIHGLGEHTGRYKAWVE
                     RFNERGVTLLAFDLPGHGHTPGRWGVIPSPEKVYDTIDSILSEAALNFPGVPLFIYGH
                     SLGGGIVLNYLIRRKPSVTGAIVTSPWVILAETLPPLKVFLAKAAGTLMPGMTQSSGL
                     KTEYLSRDPEVVKAYRSDPLVHGQISAGLYLWMSSAAEETLARAQEITVPLLLAHGRN
                     DLITSPSGSVQVAGSAPRATLKLWDEGYHELHNDPLKQEHFDFITEWMDTLI"
     gene            29693..30670
                     /locus_tag="PKK03_03220"
     CDS             29693..30670
                     /locus_tag="PKK03_03220"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HNX83435.1"
                     /translation="MDLITGVQMPAAASEISYDTPVMFLGSCFANEIGYRMAAGKLPV
                     MTNPHGTLFNPFSVAGAVDRFIAGHTYTDKDIYLHKNRYMSLDHHTAFSSYERDVLIE
                     RLNEVSLSASAFTRKASFLFVTFGTSYIYTLRESGAIVANCHKLPSSLFIRRQASWSE
                     IATRWKETLDRLAEASPSLKVWFTVSPVRHLSDGAHSNQLSKAQLLLATEELLSHPSV
                     EGYFPAYEIFMDELRDYRYYTSDMVHPSETGIDYIWEKFSSIFISAHTMRLWSEAARI
                     TRAMAHRISGGKRETAPFANAMLEKINDLKNRAPFLSLDREEDYFKKLL"
     gene            30740..33130
                     /locus_tag="PKK03_03225"
     CDS             30740..33130
                     /locus_tag="PKK03_03225"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX83436.1"
                     /translation="MKRHTLLFVFMALVYSGIYGQAGGGSIRGQVVDAATNEPVPFAS
                     VVIWNTTTGAMTDFDGNFTFTGVKPGFVELRISSVGYKPYVTDGVMVTNNNEVNLVIK
                     LESTIVEVGEIIVKPSPFRKSVESPISARIIGIQEIELNPGGNRDISRVIQSFPGVAS
                     TPAFRNDVIVRGGGPNENRFFIDNVEIPYLNHFSTQGSSGGPTGIINVDFVRSVDFLS
                     GAFPASRGNALSSVMDFTMIEGNREKRSLRATVGASDLGLTFNGPVGENSSLIVSARR
                     SYLQFLFGAIGLPFLPTYTDFQFKYKVKFDQKNELTVLGIGAKDDFSLNLEANETEYQ
                     RYILGYIPTQEQYSYTVGAVYRHFRSNGSDMLVLSRNYLNNTQYKYLNNDDSNPANKT
                     YDYQSGEGDIRLRYERTVIWPGSLRLSYGAGTEMSRYRNDTYRQFFAGDGLQTELYNS
                     NLVFFKYAAFGQLSRSFIDERLKLSLGLRTDANTFSSLMANPLTQVSPRLSATYALTG
                     ELNLNFNVGRYYQLPSYTSLGLLDNDGNIVNKDLKYIAADHIVGGVELVPDERLQFTL
                     EGFYKNYSRYPFSVSDQVPLSTKSAGYGVFGNEPLTSTAVGRAYGLELMGRARDIEGL
                     NMVFSYTFVRSEFEGTDGQYIPTAWDNRHLISVTATKSIGKSWNAGFRWRYVGGAPFT
                     PFDTDKSSYIDAWKVSGQGYLDYSRFNTERLKGFHQLDLRIDKQFYFDRWSLMLYVDV
                     QNVYNFKADQPDILVLQTDPAGSPIVNPVDPERYLLKYIDSEAGTVLPTIGIIIEI"
     gene            33144..33887
                     /locus_tag="PKK03_03230"
     CDS             33144..33887
                     /locus_tag="PKK03_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83437.1"
                     /translation="MKYFIILFAALLASCSSKPSATNLLQRQTMTDVVTNTSGRGEEI
                     EVTFYGGPSLYYPLMAVWIEDENGSYIQTLFVPKAIATGVFKYGSNASGKWVEAAKRA
                     PQTLPYWSHKRGIRSADGLFMPDQSSPVADAYSGATPTTSFVLKTRADNPLPARFRVM
                     FEVNQNWDWNEYWTNDRFPGDKNYLFNAQPAVVYEGTVDMSLLKDRYLLRPVGHSHPT
                     GATGELFSDLGTITTALQIADSVVVRIKK"
     gene            complement(33903..34793)
                     /locus_tag="PKK03_03235"
     CDS             complement(33903..34793)
                     /locus_tag="PKK03_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNX83438.1"
                     /translation="MNAGSRTYSIKDLENFCGIKAHTIRMWEKRYGILTPERSDSNIR
                     HYTEDELKKLITVSILNRNGMKISKIARLNDSELVKEVLKSGTGDESSDGTFKPGELI
                     MSALRFSEEEFREKLNQFVVEKGLAQAYLQVFHPLMQKARALWLTDCISKPQEQFIRH
                     VIRTILIAGDMSHEAHTNGQNAAIINLGDTESENNLVFLKYLLRQRGFNVVYTEGTLS
                     VEDIKGIHTVRPFTVLALNIPASEPDDEVRGFCDATARELDLKKVLVLGRYDNSGTWS
                     NGKTKAVCSPAGLAEWANSL"
     gene            complement(34845..35615)
                     /gene="kdsB"
                     /locus_tag="PKK03_03240"
     CDS             complement(34845..35615)
                     /gene="kdsB"
                     /locus_tag="PKK03_03240"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNX83439.1"
                     /translation="MKESHSPRFTGIIPARYASSRFPGKPLAVIGGMTMIERVYRQAS
                     LVLEETWVATDDERIREAVTAFGGKVVMTSSEHRSGTDRCAEAAAVVTDGSEDTVIIN
                     IQGDEPFIRPEQIRALMDCFTGNEVMIATLIRRAEPDEDLFNPNHPKVIVRKNMDAIC
                     FSRSVIPFFRDSPAAEWSRKHTYYKHIGLYGYRASTLRELTLLPQSPLEKAESLEQMR
                     WIENGYSIRCAVTPWESIGIDTPEDLDRAAKMLQEKLL"
BASE COUNT         8514 a         9164 c         9265 g         8704 t
ORIGIN      
        1 aggtctgaag gtctgaaggt ctgaaggtct gaaggtcaca aggtctgaag gtctgaaggt
       61 ctgaaggtct gagggtctga gggatttcga gttctgactt ccaattcgca attcgcaaat
      121 cacaaatcgc aaatccacta ttgccgactg ctgagtgctg actttacgaa tagcattttc
      181 caacatcatt cttatctttg tgccttcggt cagatcatca gatggcaaag aacgaaaaaa
      241 ggaagggcaa gtggaccgac aggttccgct ttgcaatatt caatgacaca accttcgagg
      301 agctgtggcg gatacgcctg tcaaaggcca acgcactgct cgcgggagcg ctgctgctgg
      361 ttataaccct cgccctgaac acttcattga ttgcattcac caatctcagg gaatttatcc
      421 cgggttaccc tgatgtagag atgcggcgta acatcctcat gaatgccatc atgctcgact
      481 ccctggaaca tgaactcgag atcagggaca aatatttccg tgacctgaat gacgtcatct
      541 ccggcaggca gcctgccagt tcggtgactc tgcgtgacag cacccgtgac tacagcaata
      601 tagagttcag gaaatcctct gaagattccc tgttcagggc acgcgtcgag aaggaggaca
      661 aatacagcct gtctgcttcg gccatatcgc cgggtatccc ttcaactggc caggtcacca
      721 gccttgccaa cattcatttc ttccccccgg ccaaggggat catctcaagt aattttgaca
      781 cacgtacacg gcattatgcc actgacatag taacccagcc aaaggcggtt gtctcatcaa
      841 ctcttgacgg aaccgtgata atgacgggtt ggaccatgga gaccggtttt gtgatcatgg
      901 ttcagcatgc caacaacctg gtgtcggttt acaagcataa cgccaggctg ctgaaggaga
      961 tgggtgacag ggtcagggca ggggagccga tatcgatagt tggtgattcc ggtgagcttt
     1021 acacttcggg gccccatctt cattttgagc tctggcataa cggagaggcg cttgacccgg
     1081 ctcagtatgt gtatttctga caggaggctg acggatgcat aaacgactgg caatcatagg
     1141 ctctaccgga tctataggca cccagaccct gggaatagtt gctcgttacc ctgatttgtt
     1201 cactgttaaa gtcctcactg caggaagcaa tgccgagctt ctgatggcac aggccaggct
     1261 ctgccggcct gagaaggttg tgataggcaa cgaggcattt tatgaagttg tgaagaccgg
     1321 tctgtcaggc ctgggtatag aggtgatggc cggccagaag gctgttgaag aggttgcatc
     1381 gtctgacggc atcgataccg tggttgcagc catggtgggt tttgccgggc tgcgtcccac
     1441 cgccgccgcc gtggctgcag gcagggaggt ggcgctggca aacaaggaga ctctggttgt
     1501 tgccggagag atgatcacac gtacagcagc cattaccgga accaggcttc tcccggtaga
     1561 ttcagagcac tcagccatca tgcagtgcct taacggtgag ccggagtcat cagttgaacg
     1621 tattatttta accgcatcag gcggtccgtt ccgcgacaca cctgcagggc ggctggcaaa
     1681 agtcaccccg ctggaggcgc tgcgtcaccc caactggacc atgggcagca agatcaccat
     1741 tgattcggcc accctgatga acaaggggct tgaggtaata gaagcacact ggctcttcgg
     1801 cactcccccg gaccgtattg atgtgctgat tcacccgcag tcaatcatac actccatggt
     1861 gcagttcact gacggatctc tcaaggcaca gcttggagtg cctgacatgc ggctgccgat
     1921 catctacgca cttacctggc cggaaagagt ggttactgag ctcccccgtc ctgacctggc
     1981 tgagacagga tcacttacct tcggggagcc tgatactgcc aggttcagat gccttgccct
     2041 cgcacgggcg gctctgaaag aaggcgggaa catgccatgc gccctcaatg ccgccaacga
     2101 agtggcagtg gcggcattcc tccgtgaaca gattggattc aatgatatag ccggcaccgt
     2161 ggaatttgtg cttgcgaaaa catcattcaa cagcgaggct tctctcagca cctatgctga
     2221 gaccgacaac ctgagccggc gcctcgcaga tgattacata aacaaagtac taaaaaaatg
     2281 acagttctga tcaaaatact gcagcttctg ctctcgctct caatcctggt aattgtccat
     2341 gagtttggcc acttcctctt cgcccgcatt tttaaaacga gggtggagaa gttctacctg
     2401 ttctttgact ggccatgggc gatcttcaag aaaaagttcg gcgaaaccga atacggcatc
     2461 ggtatgatac ctcttggcgg gtatgtcaag atctccggga tgatagatga gagcatggac
     2521 cgcgagcaga tgaaactgcc tccccagcca tatgagttca gatctaagaa agcatggcag
     2581 agactaatga tcatgcttgg cggggtattc ttcaatttca tctttgcact gctgatctat
     2641 gtgggtgtac ttagcctgtg gggtgagacc tatctctccg caaagaacgt cagatacggt
     2701 atacttaccg attctaccgg ctatgcaatg ggactgcgca acggtgataa gattatttcc
     2761 gttgacggga aagaggttga caacttttat gcaatcatac ctgacattct tttgaatgag
     2821 cgccaggtac ttactgtgga ccgcagcggc gaaatggttg atatcccgat ccgccgtgag
     2881 ttcatcccga ttcttctcaa gaatccggat attattgatg cgagaatccc cttcggcccg
     2941 tttgtagtct ccggagttgc cggcgagtcg cctgccgctg cagccggtct ggcaccgggt
     3001 gatgagatag ttgccattga cgggaatccc tttatctggt atgatgagtt ccagacctac
     3061 ctggcgcagc ataaggccag acctgtcaca gtatcagtaa aaaggggagg ggagctaaag
     3121 gatatttcag ttacaccaac cggggcgggg atcctcgggg tttttcgcga aacagccaac
     3181 atcctggagc tgagtacaaa acactacaat ttcttcgagg ctgttccggc cggtatttcc
     3241 aaaggctgga aaacgacggg cgattacctc aagcagttca agctgatctt tgcgaaggat
     3301 accaaaggat acgagtcgct cggtggattc atcaccatcg gaagcatctt tcccggaaaa
     3361 tggaactggc agtcgttctg gaacctgacc gctttccttt cactgatcct ggccgtaatg
     3421 aacatactgc caatcccagc ccttgacgga ggccatgtga tgttccttat cttcgaggtg
     3481 gtcaccggga gaaagccaag cgacaagttc atggaatatg cccagatcac cgggatggtc
     3541 atactgcttg ccctgcttat atttgccaac ggtaatgaca tcctgaaact gatcaggaaa
     3601 tgataattac ggtcaggtga acaacggaag cgaaagattt tgtacatttg cataaataaa
     3661 tcaacagaga aatgggaaag atcggacctc ttgaaataat cctgatactt ctggttgtag
     3721 tcctgatgtt tggcggaagg aagatccctg agctgatgaa gggaataggg cagggactta
     3781 aggagtttaa gaaagccaag gacaccggtg atgatgccga taagggcact gcaaaggaaa
     3841 actagggtaa taaaagctta tatttaagcc tgcatgagag tgcaggcttt ttatttcata
     3901 ataaccagac atgagcaaag cttcagtttt cctgtttctg atggtggcat tcatagcttc
     3961 gtgtggtgat tcgtcgctta caaggaccct tccgaacgtg accggcacaa ccggggaggt
     4021 tttagtggtt atggacaaat acatgtggga cggcagcccg ggcagagcca tcagggagca
     4081 ggtcagcccg ctgcttatcg gcctgcctca gcctgaaccg gcatttacca tcctgccggt
     4141 caatccttcg ggcttccgcg acatctatgt ctcgcaccgg aacattatac aggttactat
     4201 tgaccccgga attcagaagc cggtggtgac atatacccgc aacaaatggg cagagacaca
     4261 gcttgttgta agcattgcag ctgctgatac cgcttcgctt gccgcagtgg tgagggagaa
     4321 aggcgatgca atacgccgca gcatcaatga cgcggagaga gagaggctga cagcctggct
     4381 gaccaactct gcggggaagg aaaaaagcat cctgaatgca ggagaagcca gatgggagct
     4441 ggctatgcca gcaggctgga agccggattt caatcgtgag ggtatcatga tgatatcgct
     4501 tgagacccct tcaacaacac agacggtgat ggtgtctgta gctgaccgga caaccgcgag
     4561 gatcggatgc ctcgagcttg ccgatctgac gcagaagcgg atgtcagacg aggtgaaggg
     4621 ccctgacggc gggtcattca tggtgataga acagaagata ccggtcagtt gccgctcatt
     4681 ccggaggtat gacactgatt atatagaaat gaggggatta tggacgcttg agggcggctt
     4741 catgggcggc ccgttcatat catatgcctt tattgatccg gctacgacca gggcggttgt
     4801 tgtcaccggg tttgtatacg cgccgaagga ggagaagcgg gagttgcttc gccaggttga
     4861 ggcgctgatg tatgcggtaa aaaagcggca ggaataagta acaggctccc tgcgggacgg
     4921 agacaatgct taatggtctg ccaaaatcac aaatcgcaca ttgcacatct acaatgcatt
     4981 aagctcaacg agctatattg acacattatt gccaactgcc tattgcctgt tgcctaatct
     5041 actggaacat accgctgatc ccgccggacc gggatgaacc ctgcttcacg tattatctgc
     5101 tgcatttccc cggggctgac gcggaacggg tcaccggctg cgctcaccac attttcctcg
     5161 atcatcaccg atcccaggtc attggcgccg cagtgaagtg atatcatggc tgtctccttg
     5221 cccacggtga gtattgatgc ctgaatgttc gggatattgt cgagcatcag acggctgatt
     5281 gcaatcagtt tcaggtattc cggtgctgtc actgtgctgt atatgccatc cttttcggcc
     5341 agccttgttc cgcggtccat gaacggccag gggataaagg ttatgaatcc gtcgtgcccg
     5401 gccggcctgg catcctgaag tttacgcagc atgtcaagat gttcgagtcg ctccagtacc
     5461 gtttcggcat ggccgaacat catggttgcg gaggtcggca ggttgagcct gtgcgcctct
     5521 ctcatcactc ccagccattc ggcgacggtg gccttgacgg gtgaaatgag cttcctgacc
     5581 ctgtcagaga gaatctcggc gccggcaccg ggaagggaat caagccctgc attacggagc
     5641 gccaccagca catcatgata gcccatcttc tcagcccggg cgagccatgc cacctccggc
     5701 ggaccgaggg catgaagctt cacgcccggc cagcggctct tgagcctcct gaaaagatca
     5761 gtgtaaaagc cgagtcccag cgccgggttc atgcctccct gcagcagaag ctggtcacct
     5821 cccagagcca gcaactccct tatcttccgg tcatactcct catccgtggt cacataggca
     5881 tcctcgtgct tttccgtacg gcagaagttg cagaaccggc attgtgagaa gcatatgttg
     5941 gtgatgttta cattccggtc gatgatccaa cccaccctgt tacccggatg gagcctttgc
     6001 ctggtaaggt cggcaacgta catcagctcc tccggcggag cctcattcca gaggctcagg
     6061 gcctcatcaa cggtaaggcg gccacccccg gccagcctgt cgtatatctg ttctctgatt
     6121 cccatcctgt gacctcctcc gcattttagc ggatgtctgt aaaggtaata tattaaagca
     6181 atatcattca atattttaat taactttacc accctgaaca gcctctttgc tgttcctcag
     6241 acctgattta caatggataa aaaacctata gttgcaggga tcacccaggg agacatgaat
     6301 ggcatcggct acgagatcat aatgaaggcc ctgtctgatc ctgagattgc cgaactttgc
     6361 gtgccggtag tgtacggttc gccaaaagtg gcagcatatc accgtaaggt gctcaatatc
     6421 accaatttca acttcaataa catccgttcc gtcgctgaag cgcatccgaa gaaggtgaac
     6481 cttttaaact gccttggcga tgatgtgcat gtggaacttg gaaaatcgac tcccgaaggc
     6541 ggccagtcat cacttgctgc cctggaggca gcggtcagcg accttactgc cggccggatc
     6601 gatgtgctgg tgacagcgcc cattgacaag agcaacatcc agtcagacag tttcaggttc
     6661 acaggccata ctgattacct caaatcaaag accggctccc acgatgtgct gatgctcatg
     6721 atctccgaga gcatgaagat aggttttgtc accgagcaca tgcccctgaa ggatgtcccg
     6781 ggcgcaatca ccatcggaaa tatcctgacc aagctcagac tgctgaacag gtcgttgctg
     6841 caggactttg caatccgggg cccgaggata ggtgtgctga gccttaaccc gcatgccggt
     6901 gacaacggtt tgcttggcca tgaggaaaag gatataatag ggcctgccat caccagggca
     6961 aatgaagaag ggatacttgc cttcggcccg tattcctctg atggtttctt cggatcgggc
     7021 gctttcaagc ggtttgatgc ggtactggcg atgtaccatg accagggaat gggagccttt
     7081 aaggcgcttg cctttgatac aggagtaaat ttcacggccg gattgcaggt tatacgcacc
     7141 tcaccggtac acgggacggc gttcgacata acagggaaga atgaggcatc aggctactcg
     7201 atgcgccagg ccatctattc tgcagtggac atattccgga acagggagat gtacgaggag
     7261 ataaaacgca atccgctgca gccgcaaaag attgagattc atcccgaaca cgtcgacgag
     7321 cttcctcccg agaccgaaaa ctacgaccag gcattatgat cgattatatt accggcactg
     7381 tgaccgagct caatcctgcc tttgcagtgg ttgagtgcaa cggcataggc tacggcatta
     7441 atatttccct gaatactttt tctgccctcg aaaaggatac ctcctgcagg atcctgatcc
     7501 atgaggcgat ccgcgaagat gcccatctcc tgtacggttt tgcaacggca gatgagcgtg
     7561 acctgttcag gcagctcata accgtcagcg gagtaggagc ggggacagcg agaatgatgc
     7621 tttcgtcggt gactccttct gacctgaggg aggcaataat cagtggcaat gtggccattc
     7681 tcaagggtgt caagggaata ggcctgaaga ccgcacagag gataattgtt gacctgaaag
     7741 acaaaatcgg aaaacatgct gtttcgggcg aaattcttgc attttccgac aatactgcac
     7801 gcgaagaagc gttatctgct ttagtgatgc ttggatttgc caggagcagc gtctcgaagg
     7861 ttattgataa catcctcagg gaaaacaaaa aactccaggt ggaagagata gtcaggaagg
     7921 cgctgaaact gttataaaca gggtgccgga ttgcagtttt ctacacatat tagaggtctt
     7981 ttgatcaggg tggggctatt gattctcctc tccctttctt cgtttgtcat aaaggcacag
     8041 accaatcccg gcgattctct cctcaggctg aaccgtgatc cggcattccc gtgggaggca
     8101 aaaaagtcat caccgctctt cctccgcaac ccctccaaca tcaccacccg cgtcgaatat
     8161 gacggccgaa acaaccagta catcatctac cagaaggcag gcgccctcga cttccgcaag
     8221 ccggtataca tgtctgcaga agagtaccgc aggtttgaat tcaaccaggc tatgcgtgag
     8281 tactgggatg ccagcctgcg gggcgacgca accggctaca ggtcgtctct catcccgcag
     8341 atagaggtcg gcggggagac ttttgaccgc atcttcggca gcaacatcat caacatcgtc
     8401 ccgcagggat cggcagaact gatattcggc atcaacattt cacatacaga gaatcccacc
     8461 ctctccgaaa ggctgagaac cattccgaca tttgacttcc aggaaaagat ccagatgaat
     8521 gtcaccggca ccattggcga caagatgcaa ctggggatca attacaatac ggaagcaatg
     8581 ttcgagtttg aaaaccggac caagcttgaa tactccggca aggaggatga gatcatcaag
     8641 aagatcgaag cgggcgatgt aacccttccg ctcaacggca ccctgatcac cggaagctac
     8701 agcctcttcg gcctcaagac cgagctgcag tttggaaagc tgacgatgac cacagtcttt
     8761 tcacagcaga agggtgagtc gtcggtggtg gaggtgaaag ggggctcaca gataagcgac
     8821 tacgagatta ctgctgacaa ttacgaagcc aaccgtcact ttttcctctc gcaggagttc
     8881 agggagaatt ttgacggggc gctttccaat cttccgatag tcagcaccgg ccttaacatt
     8941 gagaagatcg aggtctggat aaccaaccag accagccgtt ttgaggatgt cagcaacagg
     9001 aacatcatcg ccttccttga ccttgcagag aaccaggcaa atatctacaa tacagtccct
     9061 gcgttccagg ggactcccgg tgcaccggtc aatccctcca acaacaccaa cggtctctat
     9121 gaacagctga acaccacata cggtaatgtg aggaacattg accaggttgc tgacgctttt
     9181 gccgggttat accccggatt ccagataggc cgtgactacg agaagataga gaacgcacgc
     9241 aaactgacgg aacgcgaata tacggtaaac cggcagttgg gttatatctc acttaacgtg
     9301 gcgcttaaca atgatgaagt tcttgcggta gcttacgagt acaccctgaa cggtcaggta
     9361 ttcaaggtag gcgagttctc gacagacggt atcactgcgc ctgacgccct catcctcaag
     9421 ctcataaaag gcaccgccct gagtccgcag atacccacct ggaggctgat gatgaagaac
     9481 atctacaaca tcggctcagg gaggatcgaa ccaaaaaatt tcgaggttaa catcctctac
     9541 caggatgaca agacgggcaa tgccctgaac tacctgcccg gaaccccgct ggatggccag
     9601 gtattgcttc aggctctcgg gcttgaccag ctcaactccc agcttgaccg gcagcctgac
     9661 ggcctgttcg actttgttga cggcataacg gtgatggctg aaaaggggag gataatcttt
     9721 cccgtgcttg agccgttcgg caggcacctg ctgaagtata tcactgatcc ggaacagatc
     9781 aggaagtatg tcttcaccga actgtatgac tccacccaga cggtagcaag acagatggcc
     9841 gagaagaaca agtacgtcct cagcggccag ttcacatccg catcgggctc agaaataagg
     9901 cttaacgtag ccaatattcc ggccggctct gtcaaggtta cagcaggcgg ggtcacgctt
     9961 actgaaaacg tcgactacac ggttgactac aacatgggtt cggtcaagat catcaactct
    10021 gcaatcatcg agtctcagac accggtccag gtatcacttg aaagcaacca gttcttcggc
    10081 ctgcagacca aaacgcttgt cgggacccac ctcaattacc gtctctccga caggttcaat
    10141 cttggcggaa cggtgctgag gctcaccgag aggccttaca cacagaaggt cacatatgga
    10201 gatgacccga tctccaacac catctacggt tttgacgcat cataccggtc tgagtcacag
    10261 cttttgacca atgccatcga ctggctcccg ttcattgaaa cgaacgctcc ctcatccata
    10321 aacttctttg gcgagtttgc gcatctcata ccagggcaca gcagggcaat cacatcagag
    10381 ggagctgttt acattgatga ctttgaggca agcgagattt cacttgacct cagggcattc
    10441 aatgcatggt cactggccag cgtaccccag ggcctcgatc atttcttccc tgaagcaagg
    10501 ctgagcaagg acatccgttc cggattcaac agggcaagga tggcgtggta tgtcattgat
    10561 cccctcttcc tgcgcaacgg ttcaaccacc cccgggcata tccgctctaa tcctgacacc
    10621 cagtcgagcc acttcgtgag agagatctac gagaccgaga ttttccccta caaggagtct
    10681 cccagcggca ttcccaccaa catctcggtg ctgaacgtgg cattttatcc ggatgagcgc
    10741 gggccgtaca acttcgatgt gctgcccggg gcatattcgg cagggctcag ctccgacggg
    10801 ctgctgaact caccgcgcac gcgctgggga gggatgatgc gcgagctgct gacaaatgac
    10861 ttcgagtcgg caaacatcca gtacatcaag ttctgggtaa tggatccgtt cgttgagaat
    10921 cctgaccatg aaggtggtga tctttacttc aatctgggta acatttccga ggatattctt
    10981 cgcgattcgc ggaagcagtt tgaaaacggg cttcccacct cgccgctggt gctgaatgtt
    11041 gacaccaccg tatggggaag ggttcccact gttcaggccg tggtgcacgc ctttgacaac
    11101 aacatcgagt cacgccgcta ccaggatgtc ggtcttgacg gacttggcaa ccaggatgag
    11161 atttcattct ttgacagtta cctgcaggcc gtaggggcgg ttgtgagtcc cgaggtactg
    11221 gacaaaattt ttgaagatcc ggcaaatgat gactttcact atttcagggg atctgactat
    11281 gacctgctgc agcttggcat ccttgaacgg tataagaaat tcaacggcac cgagagtaac
    11341 tcccccacat cggagatgtc aaacgagtca tatcccacca gcgggtctac cctgcctgac
    11401 atggaggata tcaaccgtga caacaccctc agcgagactg agagctatta tcagtatcac
    11461 gtgagcctga ggcctgcgga tatggttgtg ggcaggaatt tcatcgttga tgagcttgaa
    11521 tacaatgcca ccttcgccaa cggcgaaaaa tcacccgtca aatggtacca gttcaagatc
    11581 ccgatcaccg actatgaaag gatcgtaggc tcgatacgcg acttcaagtc gatcaggttc
    11641 ctccggatgt acatgcgtaa tttcgatgag caggtgatca tgcggtttgc acggctggag
    11701 cttgtcaggt cagaatggcg caaatacaac ctcacattct tcgagggtgg tgagcgcatc
    11761 actgttcccg aagaggatga cggtactttt gagatcagct cggtaagcat tgaggagaat
    11821 gccggcaagg agccggtgaa ctatgtcctg cctcccggct ttgaccgtgt cattgacccg
    11881 gccaatcctc agctgaggca gcttaatgag cagtctatgg ttcttcgcgt tcgtgagctg
    11941 tccgacggtg atgccagggc aacatataag aacgtcacac tggatatgag acagtaccgc
    12001 aggcttaaga tggaggtgca tgccgaggcg ctcatcggcg aaccgcttca cgatgacgag
    12061 gtgactgttt ttgtaaggct ggggtctgac tacaggagca acttttatga gtacgaaatc
    12121 ccggtgaagc ttactccgta tggcagatac aacaatgaca gtgaggagca gagggcggtg
    12181 gtctggcccg agttgaacaa gatagacatt gacctgtcgc agctgcttga tgccaaacag
    12241 tcgcgtgatg cggccatgcg cgttccgggt tcagctgtga gcgaggctga catctggtca
    12301 gtgcaggacg gaagggcccg catttcagtc agtggcagtc ccaacctcag taatgtaagg
    12361 gttgtgatgg ttggtgtgag gaaccccatc cgcacgcgtc gtccgcaaac tgatgatggt
    12421 gccccgagat cggcagaggt atggatcaac gaacttcgcc tgagcgattt ccgcgaagat
    12481 ggcggctggg ctgccaatgc acagatgcag gccaggctgg ctgacctcgg aacaattaac
    12541 ctcgtgggtc agacaagcac acccggatgg ggaagcattg acaagaacgt caaccagcga
    12601 agcatggagc aggtgataca gtatgacctc tcctccaacc ttgagcttgg caagttcttc
    12661 cctgagaagg caggcatccg cattccggtt tacatgggct attcggagaa ccgcatcaga
    12721 ccgcagtatg acccgctcga tcctgacata ttgctcaatg acgctcttga taacctcgag
    12781 tcgaaggaag agcgtgacag tgttctcaac ctgtcagaag agtattcgcg tcgccgcaca
    12841 ctcaccgtca gcaatgcagg taccacaaag agaggtgaga agccacatgc ctgggatccg
    12901 gccaacgtca ccgtaagcta tgcattcaat gaagtgtaca acactgatcc gaggactgag
    12961 gttgacgtgg agcgaaccta caggggtggc attgcttatg acttcgaggc acgaccgaaa
    13021 aacttcaccc cgttccagaa atcgagcctg ttcagctctt cggccttcag gctcatcaag
    13081 gatttcaaca tctatctctt tccgaagcac atcaccgtgc gtacggatct ctacaggtac
    13141 tataacgagg tcaagacacg caacataaat aatcccaatc tgctcatcga gccggtgttc
    13201 acaaaggatt ttcagtggaa ccgtttctac gatgtcaagt atgatatcac gaagcagcta
    13261 aaggtcgatt tcaccgccag cagcatagcc cgcattgacg agcctgccgg aggtgttgac
    13321 cgaaggcgtt atcccgagct cttcgatgca tggcgtgatt ccataatgac caacctggcc
    13381 gacttcgggc gcaccaccag ttattaccat ctgctgaacc tgaactacaa cattcccgtt
    13441 aacaagctgc cgctgctctc atgggtgacg tcaaatgcac gttacagcgc caggtatgac
    13501 tggctggccg gcaccatttt ccccgattcc ctgaatatca atccgggcaa caccatcaag
    13561 aactccaaca acggtcagtt cacattgcag gccaatctca ccaaccttta cagcaaggtc
    13621 aaattcctga aggaggtgga ggcttccacg cagcccggcg ccgtacgccg aatgagtctt
    13681 ggctacgagg aggtgaccca cagccgcagg ggactcaact tcaaagcagg caggccgcgc
    13741 atcattaacc ataacctaaa aacgaaggat gtgactgtca ccgtcaccgc accggatggc
    13801 accgttgtca gcggaaaatt tgatgttgtc tcagatgtgc gcatcgactt cactgccgac
    13861 gctgacgtga caaatgccag tgttgacatc aagggcaggg tggaaaagaa acccggaata
    13921 gccagcgttg cgggcaagta cgtggtacgg gggctgatgg cattgcggaa tgtctcggcc
    13981 acatatactg ttgaacaggg ccacattctc ccgggatacc ttcccggcac cactcttctc
    14041 gggcagcaga actacggagg catgtcatct ccgggctggc ggttcctggt gggcctgaca
    14101 gacgagcggt tctttgatga ggcagttgtc aacggatgga tcaccaccga caccctcctc
    14161 aacaacgcag ctgccttcag caaacgcgaa cagatcaaca tgagggcgaa tgtggagcca
    14221 tttccgggtc ttcggataga catcaccggt gaccgccgct actcagagac tgtgtcatca
    14281 tatctccggg cagaccgcaa cggcaatttc cctgacagca cccgtaacac ccgcatcacc
    14341 ggcaacttta caatctcagt ggtatcatgg ggcaccgcct ttgagaagat tcccaagtca
    14401 ggcgactatg tttcaggcac cttcgagcgg ttccgcgggt ataccaccct aatatcacag
    14461 aggagagctg atgagcgttt acgggtcgac aactcatatg atcccggatt cgaccctgtt
    14521 accggggagc cggtcacagg tccgtacgcc agcggttacg gtctcacctc ggccgaggtg
    14581 cttgtaccgg ccttccttgc agcatacaca aaaagagacc ctgacaagat atcattgtca
    14641 cccttcccat ccatgctgcg catgatgccc aactggagaa tcaacttcga gggactcacc
    14701 aagttcgagg cggtaagaaa ggtattcaac tctgtcagtc tttcacacca gtacaggtcg
    14761 acatatacca tcggctcctt caacaccagc ctttactatg accctgatga gacgggcatc
    14821 agccggatac gcgacctgaa gtcgaacttc attccacagt acgagataaa tacagtgacc
    14881 atcaatgagc agttcagtcc gttccttaac attgaccttg gctggaaaaa ctcgctgacc
    14941 acgaggatcg agtaccgcaa gtcgcgcact gtcacactga acctcaccag caaccaggtt
    15001 gccgatatca ggaatgacga gatcacgatc ggtgccggtt accggtttga cgatgtggcc
    15061 atcacgcttc gttcacgcac gggtcagcgg gcgctggaga gtgatcttaa catcaagctc
    15121 gatttctcaa tcagggacaa caagaccctt gcccgcaagc tgattgagga ggtgaaccag
    15181 ccggtggcag ggcagagggt cttcaccctg ggcgccacgg ctgactatgt cctcagcgac
    15241 aggttcaacc tgcagattta cgctgaccac acgatgaatg acccgtttgt ggccaacacc
    15301 ttcctgacct caaatacgaa cttcggattc agcctcagat tcacccttgt tcagtaacaa
    15361 ccgtggtgag accgaaaaaa agcctatatc agggttttaa tattaaaaaa gatgtatttt
    15421 tgacaacctg atacctgaag atcaaagtct aacaaaaaat aataaagaaa tgaatgtccc
    15481 gacaaacctg aagtacacca aggatcatga gtgggttctt ataaaaggaa acgaagcaac
    15541 tgtaggcatt accgattttg ctcagcatga gctgggtgat attgttttca ttgagattga
    15601 gaccgtgggt gaagagcttg gccatggcga ggttttcgga acggttgagg cagtcaagac
    15661 cgtgtcagac ctttttatgc ctgtcagcgg taccgtaaag gagaagaacg aggagcttga
    15721 cacaactgcc gagcttgtga acagtgatcc ttatggcaag ggatggatga tcagggtaga
    15781 actttcagat ccgtcagaga ttgatgccct tctcgatgcc gaggcataca agaagcttat
    15841 aggagcttaa atatcaacgc aataaaataa aaaaagctgc ggtgacgcag cctttttttt
    15901 cgcaaatcgc aaatcgcaaa tcgcaaatcc taaagtgttt tcttgacctg tacggtctgg
    15961 tacccttcaa tgacgtcacc gaccttgatg tcattgaagt tgtcaatgtt gaggccgcac
    16021 tcgtagcccg aggtcacttc cctgacgtca tccttgaacc gtttcagtga cccgaggtcg
    16081 ccggtataga ttacaatacc gtcacgaatc accctcaccc tggtgttgcg ggtaatcttt
    16141 ccgtcacgca cgaagcagcc ggcgacagtt cccacctttc cgattctgaa gacctcgcgc
    16201 acctccacgg tggccacgat ctcttccttg atctccggtg aaagcatccc ctccatggcg
    16261 gctctgatct cctcgatggc atcgtagatg atcgaataga ggcggatatc gattccctcc
    16321 ttctcggcaa gcttccttgc gctgagcgaa ggcctcacct ggaagcccac aacaacggca
    16381 ttcgatgctg cggcaagcag gatatccgat tctgaaatct gtcccacggc cttgtggatg
    16441 atattgacct ggatctcttc cgtcgaaagt tttatgagtg aatcgctgag agcttccact
    16501 gatccgtcga cgtcgccctt gacaatgata ttgagttcct ggaagttgcc gatggcaata
    16561 cggcggccga tctcgtcgag ggtgatgtgc ttctgggtac gcagaccctg ttccctctgc
    16621 aactgggccc tcttggtagc aatatccttt gcttccttgt cagactccat gacattgaac
    16681 ttgtcacctg cctggggtgc gccgttgagg cccagtatca gtaccggcat tgaggggccg
    16741 gcttcggtta tcttctgatt gcgctcgttg tacattgcct tgacatgccc gaagcagctt
    16801 ccggctatga ccacgtcacc tacattcagg gtgcctgcct tgacaagcat cgttgccacg
    16861 aagccgcgcc ccttgtcgag tgacgactcc agcacggtgc ctatggcaag tttatcagga
    16921 ttggccttga gttcaagcat ttcagcctgg agcaggattt tctcaaggag caggtcaata
    16981 ttgataccgc tcttggctga gatctcctgt gactggtatt taccgcccca ctcctcaaca
    17041 aggaagttca tgttggcaag ctcctcgcgt atcctgtcgg ggttggctgt gggcttgtct
    17101 atcttgttga tggcgaagat tatgggtacg cctgctgcgt gcgcgtggtt tattgcctcg
    17161 cgtgtctgcg gcatcactcc gtcatcagcg gccacaacga tgataacaat atcagtaatc
    17221 tgggcaccac gggcacgcat ggccgtaaat gcctcgtgac cgggggtgtc aaggaatgtt
    17281 atggtctttt caccctcaag agtgacggtg taggctccga tatgctgtgt tatgccgcca
    17341 gcctcaccgg ctaccacgtt ggtgttgcgg atatagtcaa gcagcttggt cttgccatgg
    17401 tcaacgtgtc ccatcacagt gacaatcggg ggccgcggct ggagattggt ttcatcatct
    17461 tcctcctcct tcactgcctc ctgaagctcg gcgctgacga actcgactgt gaatccgaac
    17521 tcctcggcta ccagcgccat ggtctcggcg tcaagcctct ggtttataga gacaataagt
    17581 ccgaggctca tgcaggtgga gatgatatca accaccggaa cgtccatcat tgttgccatt
    17641 tcgttaacgg aaacgaactc ggtcactttg agaacgctct tttcgagctc tttcttttcg
    17701 gtctcgtcct tcattctctg gctgatggca tcgcgcttgt cacggcggta cttggccacc
    17761 ttcgacttgc tcttgctcat caggaggttg acgtccctga tattcttatc gacatcttcc
    17821 ctgtcaactt cgggcctcag ggcgcggaac gactttttcc tgtcgccgcc gcggtggtcc
    17881 ttctgcggtc ccttcttgtc tttcctggct tcctgctggg gaggagcaac aggggtgtca
    17941 agggccactt tctcacgtgg tttgggcttg atcctttccc ttttcttgtc atgcctgtgc
    18001 ttgtcagcag ctgactggtc acggctcttc ttgacttcag ggacaagctc tatcttgcca
    18061 accattttgg gcccggcgag tttttcgtat cccggacgga atatctcagt ttcttttggt
    18121 tcctgggtta cctggggttc gggctccggc gcagccttga cgggctccgg ttcgggccgg
    18181 ggtgccgcag gctcctgctt cttcacttca ggggcagctt ctgttttctt tccggcggcc
    18241 tttttagacg gtttctcggg tttgaccagt ttgtcgaggt ctaccctgcc cactaccttg
    18301 ggttgcctgg gttcggcagg tatttcgggc gtttcggctg tgacaggtgc ttcggtcgtt
    18361 gttgtgacag ccggttccgg agcatgagca gcagcagcag gcgctttctc aacaggagct
    18421 tcgggcacct tctgcaccgg cctgacggga ggagtgaggt caaccttgcc tacggtcctg
    18481 atctcaggtt tgcgtatctc ggtccggagg tcaatatgct tcttaacccc ggaaatgtcg
    18541 gtgataagga aatcctcatc aggctcaccg ttgtcgcctt ccctgcctga ctccatatct
    18601 tcaatggaga gagacttctt tggagtaatc ttttccttaa gagccagttt ctcggcatcc
    18661 ttcttgacac tgaggtcgct gctgtattcc ttgactatca gagcgtaagc ctcaggcggg
    18721 atctttccgt tgggatcatg ggagatgtca aatcccttct tgtgaaggaa ctcgacaatg
    18781 gtctggatcc cgacattgaa ctcgcgggct gccttgctta aacgtatggc cttcttgtct
    18841 tcagtcatat atccctttat agtaacctct ttttaagtta atatattgtt gccctgaggt
    18901 tgcaggctat tctttttcaa attccgattt gaggatattg acaacctcct tgatcgtctc
    18961 ctcctcaaga tctgttctct ttaccaggtc atggacggtc agcctcagca cgcttcgtgc
    19021 cgtgtcacat ccgatggctt taagctcatc gatgatccat ccttcgatct catctgcaaa
    19081 ttcgtcgagg ttgacgtctt cctcatcctc gttatccggt tcgcggtaaa cctcaatgtc
    19141 atatccggtg agctgaccgg caagcttgat gttcagtccg ccctttccga ttgccagtga
    19201 cacttcgctt ggcttgaggt agatttcagc ttttttctca gactcaatga gttttattga
    19261 cgatattttg gccgggttga gggccctggt gataaagagc tgtatgttgg atgtgaaatt
    19321 gatgacatcg atgttttcgt tgcgaagttc gcggacgatg ccgtggatcc gtgatccctt
    19381 catgcctacg catgcgccaa ccgggtcaat gcgctcgtca tatgattcaa cggccacctt
    19441 ggctctttcg ccgggaaccc ttacgatctt cctgatggtg atcaggccgt caaatatctc
    19501 gggtacttcc agttcgaaga gtctctcgag gaagacggga gatgtgcggc tcaggataat
    19561 gttgggggta ttgttcttca tctccaccct gatgacgacg gcacggatgg tgtcaccctt
    19621 gcggaaatgg tcagaaggga tctgttcccc cttgggcagt atcagttcgt tgccatcgtc
    19681 atcaaggacg agggtttcgc gcttccacac ctggtagacc tcgccggtga cgatgtcacc
    19741 aatgcggtcc ttgtatttca gatagatatt attcttttcc aggtcgagaa tgcgtgccac
    19801 caggttttgc ctcagggcca gtattgccct gcgtccgaaa tcaaggaatt tcacctcatc
    19861 ggatacctct tcacccacct cgtaatcctc atcaatcttg tgagcttcgg agagcggtat
    19921 ctgggtgttc tcatcggtaa aagcctcgtc atcaaccact tcgcggttac gccagatctc
    19981 aaagtcacct ttgtctatgt tgacaatgat gtcaaaattg tcggccgaac cgtatctttt
    20041 ggcaagcatg ccccgaaata catcctcgag cacactcatc atggtgggcc tgtcaatatt
    20101 cttcagctcc ttgaactccg aaaaagtatc tatgagattt acattttcca ttgctgtaat
    20161 ctatactttt atttgaatga tataattact tttactgact taacatcctc aaaattaaat
    20221 gatctgactg tctctgtgat ttccttccct gttttctttt ctgtcttcag gtcaaatccg
    20281 ccggttgtca cgttcatcat ggttccttta aagcgttcgc cctcattggt gaccacgtca
    20341 accattctcc cctcgttctt catgaactgc tgcagtacca gcaggggcat atcgagcccg
    20401 ggagatgaga cgttcagctc ataatcccca acttcctcgc cgaggtcatt gcttatctgc
    20461 ctgctaagca tggcgcagtc atcaattgtg acgccctccg tcctgtcaat gagaacggta
    20521 atgcgtccgg tgctgctcag ccttacatca acaaggaaga tgccggtgcc gctgatgtgc
    20581 gcttccgcaa gtttcctgat atggtccttt gtaatcatag tctgattttt gataacaaaa
    20641 taggggactt ttgtccccta ggccattcgt acccttctgt tcgggtgcaa agataaaacc
    20701 attttttcaa ttatcaatgg atagattata tgaaaatgtg tccggagagc tgtatatgaa
    20761 gcttttcttt cccttttctg gaaactattg aataaatttg tcgccataaa acccaggctg
    20821 tttgacaaac aggagaaaga ttatcaatga cccggtatac ggatttatca gtatacccaa
    20881 tgaccttatc tatgacctca tgggtcatca gtggtttcag cggttgcgta acatcaggca
    20941 gctcgggctc tcaagccttg tctaccccgg tgcggtacac agccgttttc agcacagcct
    21001 cggggcgatg tacctgaccg ggcaggctgt tcacacactg aggagcaagg ggactgagat
    21061 ctcggcagaa gaggaggagg gggttttggc agccatactg ctgcatgaca tcggccacgg
    21121 gccgttctcg catgctctcg agcactcact gattgaggac atgcctcatg aaaccatgtc
    21181 actccttatc atggaggagc tcaacagatc catgggagga aggctgacgt ctgccattgc
    21241 catcttccgg ggagaatacc agaggaggtt tttccacgac ctggtgacag ggcagatgga
    21301 tatggacagg atggattacc tcaggcgtga cagtttcttt accggcgtga tagagggttc
    21361 tgtaggatca gacaggatta tccggatgct gaacgtctcc gacgacaggc tggtggttga
    21421 tgaaaaggga atatactccg ttgagaagtt tttgattgcc aggcgtatga tgtactggca
    21481 ggtctacaac caccgcaccg tggtctcggc tgagaaactg ctcaccagcc ttctcgtcag
    21541 ggcaaggcag gtgacggcag cgggactgaa gctgtttgcc acgccggccc tggcctattt
    21601 cctggagcca cgccacaaca gggctgaagg ggaggagagg gcatcactgg tggggcactt
    21661 caccgctctc gacgagagtg acatactctc ggcaaccaag gtctggatga gctgcggtga
    21721 cagggtgctg gaagaactct gcaggaggtt cgtaaacagg gaccttctgg caatagagct
    21781 tcagaggact ccgtttgacc cggcaagggt tagtgaaaca ggacgcatgg caaaggaggt
    21841 gctcggactg acggatgacg aggtggggta tttcgtcaac accggcgatg tgtacaacca
    21901 gacatacgct cccggcactc ctgaggtgcg gatactgctg aagaacggca ctacacccga
    21961 tatcaccgcg gtgtctgacc tctttgacaa ggaggcgctg tcagataaag taacgaagta
    22021 ttacctgtgc taccccaagg aaataattaa ataaaaaaat aactcagatg gaatttactg
    22081 ctgcaatgat agcccaaatc ctgaacggta cagttgaggg cgatccggaa acgaaactca
    22141 acacagtggc caggatagaa gagggcgggg cgggtgccct ttcgttcctt gcaaacccga
    22201 agtacgaacc ttacctgtac acaacaacct cttcggcagt gctggtgaga aatgatttcg
    22261 ctccggcgaa ggaggttaaa gccaccctta tccgggtgga tgatccatac caggcgcttg
    22321 ccaggctgct agctttctat gaacaggcca ggccggcaaa gaaaggaata catgcctcgg
    22381 cagtaatcga tgaaaaggca tcggtggggg cggacgtttt catcggagcc tgtgccgtca
    22441 tctccgaaaa cgttgttata ggtgacggat gctcacttca tccccaggta tttatcgggg
    22501 aaaatgtcaa aatcggcagg aactgcatgc ttcatcccgg agtaaaggtt tatcgtgatt
    22561 gcgtgatcgg gaatgattgt ataattcatg caggggcagt gattggcgct gacggatttg
    22621 gctttgcgcc ggtcaccgat gccaatttca tgaagattcc tcagataggc aatgttatcc
    22681 tggaagataa cgtggagatc ggggccaaca cctgcgtaga ccgcgccacc atgggttcga
    22741 caattatcag gaagggggta aagctcgaca acctgataca ggttggtcac aactgcgaga
    22801 taggagagaa caccgtcata gccgcccagt cggggctggc aggatcgacc atcgtcggaa
    22861 ggaactgcat gttcgccggg caggtgggtc tggcaggcca tctgcggata gccgatggtg
    22921 tcaagatcgg ggcacagtcg ggcataatga gcacaatcag tgaagagaac gccacttacc
    22981 tcggataccc cgcaactgac agcaaacaat ttctgaaaac cactgtttat cttaggaaac
    23041 tgcctgatct gagcaacagg gttgatgctc tcgcaaaagc agttgaatct ttaaaaaaca
    23101 gctagttccg aaaggcggtc taaaggaaat gatttgatca tcacgcagat gtagggtttt
    23161 tgattatgct gttcggaaaa ttttcttttt ctttgcccaa ttaaaactga cctgatatga
    23221 ttgaaaaaca gaggacgctg gctaatgagg tgagtctcaa cggtaagggc cttcattccg
    23281 gaattgacgt aattgtgacc ttcaagcctg ctcctccgaa ccacggctat aagttttgtc
    23341 gtactgacct tcccggcgcg ccgttaatag atgcggttgc tgaccatgtt accgagactt
    23401 cgaggggaac cacgctggta cagggtgacg cctctgttgc tacaattgag cacgttcttg
    23461 cagcatttgc agggatgcgc gttgacaatg cactgattga gattaacgga cccgaggctc
    23521 cgatcatggg gggcagttcc cgaatgttcg tggaagccat aacgaaggcc ggaatcattg
    23581 aacaggaaga ggagcgcaac tattatgtaa taaaggagaa gatagccttc tctgacgagg
    23641 aacacggggt tgacctgata atctaccctg atgatcattt cagcgcacat gttctgatag
    23701 actataactc gaagatactc ggcaaccagt atgccatcct tgatcagatc aatgactttg
    23761 aaaaggatat agcaggcagc cgtacctttg tcttcttcca tgaacttgaa ccgctcttcc
    23821 ggatgggact gataaaaggg ggtgatcttg agaatgccgt ggtgatccta gagcgtgaag
    23881 tggaacagga agagattgac cgcattgcca agatgttcaa taagccaggt atcactgaac
    23941 accatgccgg cgtactcaac aacaccaagc tcaggtatcc gaatgagccg gcgagacata
    24001 agctgcttga cctgataggc gaccttgcac tggtggggca gcccatcaag ggcaaggttg
    24061 tggcaacccg cccggggcat tattccaata ccaggcttgc caggctgatc aggcaggaga
    24121 taaagagaca gacaacaaaa aaggagattc cccttcttga taccaccaag ccggcggtga
    24181 tggacataca gcagatcaag aagctgcttc cccaccggtt ccctttcctc atggttgaca
    24241 ggattcttga gctcaccacc agccatgtga taggaatgaa gaacaccact tacaatgaag
    24301 ccttcttcca gggtcatttt gagcaggagc atgttttccc gggtgttctc ctggtggaga
    24361 cgatggcgca ggttggagga atacttgtgc ttcacaacgt ggacgagccc gaaaggtact
    24421 caacctattt cctgaagata gacaggatga agttcaagca taaggtcatc ccgggtgata
    24481 ccattatcat aagggcggat atgaccgaac ccatccggag gaacattgtg atcatgtatt
    24541 gccaggcttt tgttggcgaa aagctggttg cggaagggga gatgacggca caggttataa
    24601 agaacaagta acaagatatg atttcttcac tggcacatat tgacccgaag gctcagcttg
    24661 gcaagggggt cactgttgag ccgtttgcca ccatctatgg tgatgttgtg atcggcgacg
    24721 gtacctggat cggttccggg gcggttatct atgacggtgc gaggataggc agcaactgcc
    24781 gtatcttcaa cggcgcctcc atctcggcca ttccgcagga tctgaaattc agcggggagg
    24841 agaccaccct ggagattggt gacaacacca ctgtcaggga gtttgccact ctcaacaggg
    24901 gcacgcgtgc aagaggcagg accatcatcg gaaagaactg cctgctgatg gcttatgttc
    24961 atgtggcgca tgactgcagc ctgggagaca atgtgatcat ggttgcctca tccgggatag
    25021 ccggtgaggt ggaactgggg aactgggtga cgctgggcgg gggaaccata gtacaccagt
    25081 ttgtccgtat cggtaatcat gctttcctgg gaggaggctc aaaggtgcgg atggatgtac
    25141 ctcccttcat caaggctgac cgtgagccgc tggcctacat gggaataaac agtgttggac
    25201 tggaacggag gggtttcaca aaggagaaga tgcatgagct tcatgagatc tacagggctt
    25261 atttcaatat ggggatgaac ggcaccaaag cgctggagta cattggtgcc aacttcccgt
    25321 ccacgccgga gcgcgattac attctggatt ttattagcaa atctgccagg ggggttatca
    25381 gggggcgtta agcccctttt ttattgcctc actgcaagta agggaatcat gcatgactga
    25441 tcacccctga cctgtgattc tgtatctgtg atgagaggtg tataaaacac gaatgggaac
    25501 catactgtac gtaatagttc ccattccggc gagtgacagc catgaccgtc attgccgcca
    25561 tgctactgtt attgcagccg gttcatctct cttactcctc cctttcccgg tctgtcgggg
    25621 tctgccgtta agctgactgc tgacctgttc cggtatctct tctgttatcc gtagtggcct
    25681 tgcggttttc cgggcgcata ctgagatgcg gagtcatgaa cccttgaagc acgacacccc
    25741 ccgaaagggc agctgctgtc atgcccggat catctccatc ctatcctcgc cggttcggtt
    25801 ggcatgatga ccctctctct gaacggacac atctgctgct ttattagctt tcagccttgc
    25861 atctgtgttg ctttcctctc gcggctgtta caccgtctga tgattgctcc gtcaggccgc
    25921 caggatggga aggcttctca gcctgcaccc gtgacggacg cttctgaacc gtagttcgtc
    25981 agcgtgagac tctgaagtct gatgttaatt cagagtgata attaaaccaa tccaaagaac
    26041 cttcatgctt caaagcgact ctttatcgct cacatggtag aacaaatata acccgatatc
    26101 ccgtaacggt caagtttttc agggcaataa tacgacgaag aaagtaacat aaaataaaca
    26161 ggtttatcaa caatgataaa tggctgtatc ataagatttt aatttaaaaa gaacgaggtt
    26221 atcaacagct gttgataacc tcgtctgtca gtcgtttgac aactatctgt tattcaagta
    26281 taactttctt gttcttcggg tacttcacgc tgtaggtaaa gacaatttct ttggtttcgc
    26341 ggggtgcaac cgggaggtcc caggtaacct cgcccgtggc gctgtgctgt ttgccgccgc
    26401 tgagctcaac ggcttcaacg gtgatgtcgc tgttttgggg cagaggcagc tggtcacgca
    26461 gtctcactgt cacagccttg ctcttgttgt ttctgacgga tatcaggaac gatagggtct
    26521 caaccctgtt ggcaccgatc attttctgcg aggtgaaatc ggtgcggcgg tctcgcttca
    26581 ctgtaatgct gttgtcagca ccgagtgaca ccggcagggt atcagtcagc tccgcggtgt
    26641 tgatgtatcc cttgccggtg aaggtgttgc tgaagtaaat gtttgactca cccggcagga
    26701 ggttgtattt atcccattca gtgatgtacc ccatcaggta tgccgaagag gcaatcttgg
    26761 gcgttgcggc atagctgtag gtggcaggca cggtgagtct ctgcagctct actgtctcct
    26821 gcttgccgcc tgatgcaatg gtcttgggca catttatatc gaaactgaag ctgatgtttg
    26881 actctgttac tgtaacaggc atcggggcgc ttgcctccag atccatcatc acggcatcct
    26941 ctgccttggc ctggcgggcc ggtctgccct ttgtcccgta accgacgaca accatttcat
    27001 tgagagcgta ttcctggtag aagtcaatga accatgggtc aaggacgggc agataccctg
    27061 ctgtcatcgg tgcggcgttg gagaggctta ctttcacgtc cttccactcc actccgctgt
    27121 tctgccagat atttgcctta tagatgatta tggccggatc ggtgatgtca tcaaccctga
    27181 tatcatatgc cggctgccat cctgcattca taaccacata agatatatta agcctgcctg
    27241 ttacgggctt gctgccggaa agggtcatga caatctcgcc ggttggcatc ttcgagcggt
    27301 cgatggtgcc ggcaagctgc tggtcaagct tttccttttc ctccctgtat tccttcaaaa
    27361 tcctgttttt tttgagaatg gcagttttta cactctccat gccggtgcca tagatatcca
    27421 tcagggcctt cagctggtca ggcgttatgg ccgacttctg gcttacaacg tcatagttgg
    27481 ccttgaggaa tcgctccttc tccagcagca cctccgtggc ggtttcctca tcctcgatcc
    27541 tgatctgcag ggcctttatc ttgtcgcgca gagtgctgat ctcatctgac tctgcaggat
    27601 tctcaaggta attgttgcgg tgagtcacac ccattatcat gaaatcgccc tcacctctca
    27661 cctggatgct gttgggatct atatatggcg aaagacctcc ggcaacgtac tcccatgtac
    27721 cgggctgaag cgtgacggct gcatcaccgg agagctgggc accgcgactg aaaaccgtaa
    27781 catgacttac aggtacctgt atatactttt cctgcccctc aagagtggaa gaagaaagca
    27841 ctattgcaaa aaacgcaacg aagagatatt ttttcatgct gtcgaatttt ttataaaggt
    27901 attgaaaaag agatttatga tcaaggacga caaaaaccct gccgtaaaaa aaactgccgg
    27961 acgattgcaa tccggcagtt catcttcacc atctgtttaa cctaaccatt gtaatcttta
    28021 agaacactca tgagtttctg tcgtgcgaag aaaatccggc ttttgaccgt gccgagcttc
    28081 aggttgagat tctcagctat ctcctcgtat ttgaaaccgt caacatgcat gcggaagggg
    28141 agacggtaat cctcatgaag ctcttcaata gccttctcaa tctcattacc tgcatatgac
    28201 gactcgggag aaatgcttcc cttgtcggag ggcagattga ggtagtaaag ctcctgcgtg
    28261 ccgtccatca tcgtgttttc cctcatcttg cggcggtaat tgttgatgaa ggtgtttttc
    28321 attattgtga agacccaggc cttgaggttc gtggcatcca cgaatttgtc ctggttctgt
    28381 attgccttta aaaaagtatc ctgaacgaga tcaagcgcgt tgtcgcggtc gagggtcaga
    28441 ctgagggcgt acttctgaag attgcccttc agatctatta tgcttgatgt gaattcctgt
    28501 gcagtcatct ctctgatttt tttgtttgtt gacacaaagt taaacactga aattcggata
    28561 tccaaatctg aatatcttaa attatagtta aagcagcttg tggcacctgt tttctaaact
    28621 ccaattcaaa caaaatcagg aatataaatg tgtgatgtct aaattaagat aataaggtat
    28681 ttttttaatt tcaacctgtc cgggacaggg cgagaggagt gggggcaagt ggttaatatg
    28741 gcataaaaag cacattaaca gcacgatacg tattgaaatg aatatgatga ctttgttcaa
    28801 tcatcctctg cgcgtgagcc ccttccgctc tttcccggtt ttgattatct ttacaaaaaa
    28861 gtggctatgg aattgagaaa tttaccctct ggaaagactg ctttttaccg gtttgaggct
    28921 gcaggagagc cccggtcggt cattctgctt atccatggat tgggagagca tacaggcagg
    28981 tataaggcat gggttgagcg gttcaatgag aggggggtga ctcttctggc gtttgacctg
    29041 ccgggccacg ggcatacacc cggccggtgg ggtgtgatac catcgcctga gaaggtttat
    29101 gatacgattg acagtatact cagtgaggct gccctgaact tcccgggcgt gccgcttttc
    29161 atttatggcc acagccttgg agggggcata gtgctcaact atctcatccg cagaaagccg
    29221 tcagtgaccg gtgcaattgt aacatcaccc tgggttatcc ttgctgaaac gttgccgccg
    29281 ctgaaggttt ttcttgcgaa ggcagccggt acactgatgc cgggcatgac ccagtcatca
    29341 gggctgaaga ctgaatacct ctcgcgtgat ccggaggttg taaaggctta ccgtagtgat
    29401 ccgctggtgc acggtcagat ctctgccggg ctttatctat ggatgtcatc agccgctgaa
    29461 gagacacttg cccgtgcgca ggagatcacc gtcccgctgc tgctggcaca cggacgcaat
    29521 gacctgatca cctcaccgtc agggtcagta caggtggcag gctcggctcc ccgggctacc
    29581 ctgaagctgt gggacgaagg ctaccatgag ctgcacaatg atccgctgaa gcaggagcac
    29641 ttcgatttca tcacggagtg gatggataca ttaatctaat tcttttccgg ttatggacct
    29701 tattaccggc gtgcagatgc ctgccgcagc ttcagagatc agttatgaca ctccggtgat
    29761 gttccttggc tcatgctttg ccaacgagat tggctaccgg atggcagccg gcaagcttcc
    29821 ggtgatgacc aatccccacg gcactctctt caatcctttt tcagtggccg gagctgttga
    29881 caggttcatc gccgggcaca cttacaccga caaggacatt taccttcaca agaacaggta
    29941 catgagcctt gatcatcaca ccgcgttctc atcatatgag cgtgatgtac tgattgaaag
    30001 gctgaacgag gtgagcctct ctgcttctgc attcacaagg aaggcttcgt tcctttttgt
    30061 cacatttggc acatcctata tatatacact cagggaaagc ggagccattg tagccaactg
    30121 ccacaagctc ccttcctcgc tgttcatccg caggcaggcc tcatggtccg aaattgccac
    30181 ccgctggaag gaaacactcg accgcctggc tgaggccagt ccctcactga aggtatggtt
    30241 caccgtcagt cctgtccgcc acctgagcga cggcgctcat tccaaccagc tgagcaaggc
    30301 tcagcttctg ctggcaacag aggaactgct ctctcatcct tccgttgaag gttacttccc
    30361 ggcctacgag atattcatgg atgaactccg cgattaccgt tattatacct cagacatggt
    30421 tcatccctcc gagacgggga tcgactacat atgggaaaaa ttcagcagta tatttatctc
    30481 agcccatacc atgagactct ggagcgaggc ggccaggatc acgcgggcca tggcacaccg
    30541 gatctcgggc ggaaagcgtg aaactgctcc cttcgccaac gccatgcttg aaaaaatcaa
    30601 tgaccttaaa aaccgggccc cgtttctcag ccttgaccgg gaggaagatt acttcaaaaa
    30661 acttttgtag ataaaaaatc ataaataatt aaacaaaatc actatccggt tgtttttgtt
    30721 tcatgaaatc ttaacagaga tgaaacgtca taccttatta tttgtgttta tggccctggt
    30781 ttattcaggg atttacggcc aggcaggcgg aggctcgatc cgcggccagg tggttgatgc
    30841 tgccacgaat gaaccggtac cttttgccag cgtcgttatc tggaacacca caacgggtgc
    30901 aatgacggat ttcgacggca acttcacatt caccggggtg aagcccggtt ttgtggaact
    30961 gaggatctct tccgttggat acaaacctta tgttacagac ggcgttatgg taaccaacaa
    31021 caacgaagta aacctggtta tcaagcttga atccacgatc gttgaggtgg gtgagatcat
    31081 tgtcaagccc tcaccattcc gaaaatcggt cgagagcccc atctcggcaa ggatcatagg
    31141 gattcaggag atagagctca atccgggagg caaccgtgac atttcacgtg taatccagtc
    31201 attccccggc gtagcctcca cacctgcatt caggaatgac gtgattgtca ggggaggcgg
    31261 gcccaatgag aacaggtttt tcattgacaa cgtcgagata ccctatctta atcatttttc
    31321 cacccagggc tcttccggcg ggcccacagg cattatcaac gttgattttg tcaggtcggt
    31381 tgatttcctt tccggggcat tcccggcttc gcgcggtaac gcactgagtt cagtgatgga
    31441 cttcacgatg attgaaggca accgcgaaaa gagatcactg agggcaacag tcggtgcatc
    31501 tgacctgggt ctcaccttca acggcccggt tggcgaaaac agctcgctga tagtttcagc
    31561 ccggagatca tacctgcagt tcctgttcgg cgctatcggg ctgcctttcc tgcccaccta
    31621 taccgatttt cagttcaaat acaaggtgaa gttcgaccag aagaatgaac tgacagtgct
    31681 tggcatcggg gcaaaggacg acttcagtct taaccttgaa gccaatgaga ctgaatacca
    31741 gcgctacatt ctgggttaca ttcccaccca ggaacagtac agctataccg ttggcgccgt
    31801 ttacaggcat ttccgttcaa acggctccga tatgctggtg ctgagccgaa actatctgaa
    31861 caacactcaa tacaaatatt taaacaatga tgattccaat cctgccaata aaacctatga
    31921 ctaccagagt ggggaaggcg atatccggct gaggtatgag cgtacggtga tctggccggg
    31981 ctcattgagg ctgagttacg gggccggcac cgagatgtca aggtaccgca acgacaccta
    32041 caggcagttt tttgccggcg acgggctgca gacggagctt tacaactcca acctggtttt
    32101 cttcaagtac gccgctttcg ggcagctgag ccgttcgttc attgatgaga gactgaagct
    32161 gtcgctgggc ctgcgtacag acgccaacac attttcatca ctgatggcca atcccctgac
    32221 ccaggtttca ccgcgcctgt cggcgacata tgcccttaca ggcgagctga acctcaattt
    32281 caatgtgggc cgctattacc agctgccgtc atatacttca ctcggtctgc tcgataatga
    32341 cggcaacatc gtcaacaaag acctgaaata tattgccgct gatcatattg tgggcggtgt
    32401 tgaactggta ccggatgaga ggctgcagtt cacacttgag ggtttctata agaattattc
    32461 aaggtatccg ttctctgtgt ccgaccaggt tccgctgtca accaagagcg ccggttacgg
    32521 cgtcttcggc aatgagcctc ttacttcaac agccgtgggc agggcatacg gtcttgagct
    32581 gatgggacgg gcgagggata ttgagggact caatatggtc ttttcataca ccttcgttcg
    32641 cagcgagttt gaaggaactg acgggcagta cataccaact gcctgggaca accggcatct
    32701 tataagcgtg actgccacga agagcatcgg caagagctgg aacgccggtt tcaggtggcg
    32761 gtatgttgga ggggcaccct ttacaccctt cgatactgac aagtcctcat acattgacgc
    32821 ctggaaagtg tcaggtcagg gctatcttga ttacagccgt ttcaacaccg aacggctgaa
    32881 gggatttcac cagctcgacc tgcgcataga caagcagttt tatttcgaca ggtggtcgct
    32941 gatgctgtat gtagatgtgc agaatgttta caacttcaag gctgaccagc ctgacatact
    33001 cgttctgcag accgatccgg caggatcacc gattgtcaat cccgttgacc cggagcgcta
    33061 cctgctgaaa tatattgact ctgaggcagg aacagtactt cccactatcg ggattattat
    33121 tgaaatctaa aaaactggaa gagatgaaat actttataat tctatttgca gcactattag
    33181 catcatgttc atcaaagcct tctgccacca acctgttaca gaggcagacg atgactgatg
    33241 tcgttaccaa cacctcaggc cggggcgagg agattgaagt aacgttttac ggcggcccct
    33301 cgctttatta cccgctcatg gctgtctgga ttgaagacga aaatggcagc tacatccaga
    33361 ctctgtttgt cccgaaggct atcgccacag gtgttttcaa gtacggatcc aatgccagtg
    33421 gcaaatgggt tgaggcggca aagcgtgcac cacagacgct tccctactgg tcgcacaagc
    33481 gtggtataag gtcagctgac gggctcttca tgcccgatca gtccagcccg gtggctgatg
    33541 catacagtgg tgcgacgccc accaccagtt tcgtgctgaa aacccgggca gacaatcctc
    33601 tgccggcccg cttcagggtt atgttcgagg taaatcagaa ctgggactgg aatgagtact
    33661 ggacaaatga caggtttccc ggagataaga actacctttt taacgcccag cctgccgtgg
    33721 tctatgaggg tactgttgat atgtcactgc tgaaggacag gtatcttctc aggccggtcg
    33781 gccacagtca tcccacaggt gctacgggtg agctcttcag tgaccttggc acgataacaa
    33841 ccgcgcttca gattgctgac tcggttgttg taagaataaa gaagtaagaa ataaaatcag
    33901 aatcagaggc tgtttgccca ctctgccagt ccggcaggag agcagacagc tttcgttttg
    33961 ccgttgctcc atgttccgct gttgtcatac cttcccagga cgagaacctt tttcagatcg
    34021 agttcccggg ctgtggcatc gcagaagccc cggacctcat cgtcaggttc tgatgccggt
    34081 atgttcagtg ccagaacggt aaagggtctt actgtatgaa ttccttttat gtcttccact
    34141 gaaagagtcc cttcggtgta taccacgttg aaacccctct gtctcagaag gtatttaaga
    34201 aagaccaggt tgttttcgct ttcagtgtcg ccaaggttga ttattgctgc gttctggccg
    34261 tttgtgtgtg cttcgtgtga catatcgccg gcaatgagga tggtcctgat aacatgcctt
    34321 atgaattgtt cctgtggttt tgagatacaa tcagtgagcc aaagcgccct tgccttctgc
    34381 atcagcggat ggaacacctg gaggtatgcc tgtgccagtc ccttttcgac cacgaactgg
    34441 ttcagttttt ccctgaattc ctcttcgctg aaacggaggg cagacataat cagttccccg
    34501 ggtttgaatg tgccgtcgct gctctcatcg ccggttccgg acttgagtac ctccttcacc
    34561 agctcactgt cattcagccg cgcgatcttt gatattttca tcccgttgcg gttcaggatg
    34621 gaaacggtaa tgagcttctt aagctcatct tcggtataat gccgtatatt ggaatcactc
    34681 ctctctggtg taaggatccc gtacctcttc tcccacattc tgatggtatg tgcctttata
    34741 ccacagaaat tctccagatc cttgatggag tatgttctgc ttcctgcatt catctctctc
    34801 aatgttactt actcagtatt ataacaaaga gagagagtaa aagtttagag taatttttcc
    34861 tgcagcatct ttgccgccct gtcaaggtct tcgggggtgt caatgccgat gctctcccag
    34921 ggggtgacgg cgcagcggat ggaatagccg ttttctatcc atctcatctg ttcaaggctc
    34981 tcggcctttt cgagcggcga ctggggaagg agtgtcagtt ccctgagggt tgaggcgcgg
    35041 tagccgtaga gaccgatgtg cttgtaatag gtgtgcttcc ggctccattc tgctgcaggt
    35101 gaatcacgga agaaggggat gactgaccgg ctgaagcata tggcgtccat gttcttcctg
    35161 acaatgacct tgggatggtt gggattgaaa agatcttcat ccggttcagc cctgcggata
    35221 agggtggcta tcatcacctc attaccggtg aagcaatcca tcagagccct gatctgttcc
    35281 ggcctgataa atggttcatc accctggatg ttgataatga ctgtgtcttc gcttccgtcg
    35341 gtgaccacag ctgctgcctc agcacagcgg tcggttccgc tccggtgttc cgatgaggtc
    35401 attactacct tcccgccgaa ggcggttacc gcttcgcgga tgcgctcatc gtcagtggcc
    35461 acccaggtct cctccagcac cagtgaagcc tgccggtaaa ctctctcaat cattgtcatc
    35521 ccgccgatga cggcaagagg ttttccgggg aagcgcgacg aggcatatcg tgcgggtata
    35581 atccccgtaa accgcggtga atggctctct ttcatggttc caaattacta aaaaacagct
    35641 ttaacat
//
LOCUS       DAOPXU010000019        35427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_1108_length_35427_cov_11.458102, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000019 DAOPXU010000000
VERSION     DAOPXU010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 35427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35427
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1108_length_35427_cov_11.458102"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /gene="rpsF"
                     /locus_tag="PKK03_03245"
     CDS             <1..236
                     /gene="rpsF"
                     /locus_tag="PKK03_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNX83440.1"
                     /translation="EENWGLKKLAYPIQKKSTGFYYLIEFRGQGELIDKLEVQYRRDE
                     RIIRFLTFRMDKYAVEYAEKKRKMKETEKVREE"
     gene            239..508
                     /gene="rpsR"
                     /locus_tag="PKK03_03250"
     CDS             239..508
                     /gene="rpsR"
                     /locus_tag="PKK03_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNX83441.1"
                     /translation="MAQNNSEIRYLTPPTVEIKKKKYCRFKKNKIKYIDYKDPEFLKK
                     FLNEQGKILPRRITGTSLKYQRRVAKAIKRSRHLAMLPYVADLLK"
     gene            521..967
                     /gene="rplI"
                     /locus_tag="PKK03_03255"
     CDS             521..967
                     /gene="rplI"
                     /locus_tag="PKK03_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNX83442.1"
                     /translation="MKIILRQDIEKLGQKNDILTVKTGYARNYLIPKGLAVQATDSAL
                     KIHNENLKQRAHKEEKIRGEAEKLAQKLAGVKLVIGAKVSSAGKIFGSVNTIQLAEAL
                     NAKGYEIDRRNILLGEDAIKEVGSYKAVVKLHRDVKVDLEFEVVAE"
     gene            complement(1060..1437)
                     /locus_tag="PKK03_03260"
     CDS             complement(1060..1437)
                     /locus_tag="PKK03_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNX83443.1"
                     /translation="MKRIISTTGAPGAIGPYSQAVEAGGTLYISGQVPIDPATGKMVE
                     GGITEQTTQALKNIRAILTAAGYTMNDVVKSTCLLSDMSDFKAMNEVYSQFYTADQPA
                     RAAFAVKGLPLGALIEIETVAVK"
     gene            complement(1457..4192)
                     /locus_tag="PKK03_03265"
     CDS             complement(1457..4192)
                     /locus_tag="PKK03_03265"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNX83444.1"
                     /translation="MAIFMPLLFQASLLAQEPAQPVTAGKNLPDTVLKSLPDTTVTGL
                     NEVNPSPAFVNDTLFPGEFTTITDTSLTKTRLIISPDAVDEPIVYTSEGYMKTDMRSK
                     KVSLVENAKVTYGTIELTADSIVLDMETGSVYATFRRDSTGKAVGLPVYKDGSEEFES
                     KELNYNFKSKKGVIRNVTTEQEGGFLQSLTTKRHEDGTLHVNRSKFTTCEAEEPHFYL
                     SLPRAKVYPGEKIVSGPAYMVVADIPLPLILPFGFFPVQQKRASGIVMPKYGQEARRG
                     YFLSNGGYYFALSDYFDLKLTGTIYTNGTWLADAATSYRLRYRYSGSFGFSYANNVTS
                     YKGLPDYGKNTNYRISWSHSQDTKANPGSRFSASVNMSSNGYDRNNSYEVADHVTTTR
                     QSSISYTKSWAGAPVSFSTSINQSQNVQNKTMMLNLPRGSLNVSRIYPFKPKKPVGKT
                     RWYHDITTQYTASFENKIDTYDSLLFTSAVWKNMKNGFKHEVPLSLQFRPFNNFSISP
                     SLRYTGVLYTQQITKRWDPDYYDETRNMVVPSVVNDTLRGLTYGHALVPTVSAGFNPS
                     VYGTFSFTKKGSRLESIRHVMKPSVSFSYSPETDKLSSDMYRTVQYDTVGRTREYSIY
                     EGSIFGTPSTGTRSGSVAFSLSNIVEAKVYSRNDTTGKPRKIKLIDNLSLNTSYNLFS
                     DSLNWSPVSVSFRTTLAQNINIQANGSFSIYGMNATGGAVNELAISQGLGLARMTGFN
                     MSFDLDLGQLLGNKDKKQQSGGQGGQMSAGRQPGDPGAQGGAAPGNLPLSNRNLDEFG
                     YVRFDVPWSLRMAYNFSYSKPGLRPSISQAMTLSGDLKLTPKMALNYNTGYDFKQKEI
                     TMTRVGISRDLHCWEMSFSWIPTGYMKSWNFTIRAKASMLQDLKYERRKDYHENY"
     gene            4377..5624
                     /locus_tag="PKK03_03270"
     CDS             4377..5624
                     /locus_tag="PKK03_03270"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNX83445.1"
                     /translation="MNIKSQPLPSEDEWVVVIDAGHGGRDPGAVGAKAKEKNINLAVA
                     LKTGRYISDNMKDVKVIYTRDDDTFLGLDERAEVANRNKADLFISIHSNAMPDKRFTG
                     AETYVLGQTMDEANLQVAMKENSVITLEKDYETKYEGFDPNSVESYIIFSLMQNTYLR
                     QSTEFATMIQSQFRDRVGRKDRGVRQAGFQVLWRTTMPSVLVEMGFITNAEEEKFLVS
                     EQGQDYLASAIFRAFRDYKQTIDSRTGVRNGKVVTQAAAPVAGNEDSTAGKGTQVPSG
                     QQEARPAVSAAGNEDKAVDQPVRSDIVPSETAVKNDSGAAAAKPVLISPAPADDIYFM
                     VQVSAMPKNRELTQSQLEGLDPISRIEDGERIKYASGKFVLYDDALKHRRTLTGRYPD
                     AFVIAVRNGKIMPLREAIERQKR"
     gene            5635..6576
                     /locus_tag="PKK03_03275"
     CDS             5635..6576
                     /locus_tag="PKK03_03275"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNX83446.1"
                     /translation="MKISREVKVGAVALITIIAFILMFEFLKGTALFTSTDTYHIVYD
                     NIAGLTESNPVEINGYQAGVVQDVKLVNDGSGRILVSLSVDKHFNIPRDTEAEITTAT
                     LIAGMKIVLRMGESSEMCHNHDTISGYVATSILDRLSETISPLEGNITSIITRLDSVV
                     SALNSVFTPEMTGDIRSTMANMSDVTAGLKQISDSRKDELMAAISEIQAFTAMLSSNS
                     STIDSTLKNISGISEAVARADLETSLASLRSSLNGLDTIVTGISRGEGSAGKLVTSDS
                     LYTNLSNSLRSLDLLLRDMKENPKKYVHFSIFGRKSE"
     gene            6902..8332
                     /gene="dnaA"
                     /locus_tag="PKK03_03280"
     CDS             6902..8332
                     /gene="dnaA"
                     /locus_tag="PKK03_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNX83447.1"
                     /translation="MNKSHDEVWNNCLEIIRDIIPSASFKTWFEPIVPLRLEKNILTI
                     QVPSPFFYEYLEEQYIDILRKTLRREIGNDAKLEYNIIMENSGYANGKPYTVRYPSRN
                     KADLQNKPMQVPFEVTQPAIRNPFIIPGIKKLHFDPRLNPDYSFNNFVEGECNRLARS
                     AGIAVGNNPGGTAFNPLMIYGDSGLGKTHLVQAIGILVKEKYPDKTVLYVNANKFQTQ
                     FVESIRNNNKNDFLHFYQMIDVLIIDDVHEFAGKEKTQDTFFHIFNHLHQSGKQLILT
                     CDKPPVELQGMEQRLLSRFKWGLLADLQKPDFETRLAILRKKAYNDGIELPDEIFEFL
                     AENISSNIRELESGLISLYAQSTLNRKEVTLELARQMVDRLVNTQRREITIDYIQKVV
                     CEYYKIPVDLMQGKTRKREIVQARQVSMYFSKNLTKASLASIGSSIGGKDHATVLHAC
                     KTVNNLIDTDRHFRNQIMEIEKKFKV"
     gene            8329..9495
                     /locus_tag="PKK03_03285"
     CDS             8329..9495
                     /locus_tag="PKK03_03285"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNX83448.1"
                     /translation="MKSDSDRLKEEFFRLYGDRDKEPVLFYSPGRVNLIGEHTDYNGG
                     YVFPCALDFGTTLLVRPGSGQEIRMNSLNFPGEVVIPLALNYMPIPKSWTNYPVGVIN
                     EFARKGIGAPAMEMLFTGDIPNGAGLSSSASIEMVTAVAMNELTGAGLEMMEMVKMGQ
                     KAENGFVGMNCGIMDQFAVGFGRANHAINLNCDTLEYRYAPVNLGDRKLVITNTNKRR
                     GLTDSKYNERRAECEKAVALISLHRPVRNLSELTVSDLWMPDSYIPDPVVRKRAKHVI
                     TENGRTLDAIKALENNDIELLGILMNESHDSLCRDYEVTGAELDTLVYEGRKLPGVVG
                     TRMTGAGFGGCTVSIVEADHVDEFISRLGEIYTAKTGLTADFYLPGIGDGARRI"
     gene            complement(9588..12362)
                     /locus_tag="PKK03_03290"
     CDS             complement(9588..12362)
                     /locus_tag="PKK03_03290"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNX83449.1"
                     /translation="MCSFRFRFSLFFFIVCLSLNGQSLINLGIIRDNGASAVSLILDD
                     SSGVNLDTPRPLFSFRLDDTYFESDDVPAGLVASRFLMIYPGGAEAGYTPRGGFHPGF
                     MAELVLRNNWNDTITVTNVVPFGEKENKAFITGAGPRDLARANLFLPGKSPVRVILPD
                     NAWEMGFSTFDAAPGLSLASIARRIRTDNGIIRRYETILPPGASVTYHIHADLYKGEW
                     QEGLRLMFAGRWLHDLENFDETLYDREDLKWIRTSYLMILQMAWDRELRDRFTGNEAF
                     EGYLAEYNRLFGHLDVYGIWPTWPRLGLDDRNQWDLYRDLPGGTDALRRLSREARSQG
                     CRFFIAYNPWDKSTREEDHLHGMTQIIAETEADGVVLDTEGRSSSELQQAADTVRSGV
                     VMYSEGMPVVRDMPGIVAARVHNALYLSPELNLNKVIRPDFAIFRVADVGEDVLHREI
                     SVAFFNGYGTELNMFRPGGRGDEFRKDLDYLAQTTYLLRRNSDAFAGGNWTPLIGSGA
                     DRVYVNSWNAGEKKVFTVLNMDHRGYKGPLFSPGDTTGKHLVSLFRHEELTPSVINNT
                     LTAPVDLPGWSQAFSGTRRESSIDCVALLPRLITAEFHGSRIRIASSAGGMLLVSRGD
                     PGYTNEPLRITAPADTLVDADALLGIAGDKIVIELTDSEGILLDERVIRPEQGRPWLV
                     SNTFRTLPAPEIPAGMVLVPAATIRYNLKAVDEFVPYPRTGKEITSAVDSFLIDRFPV
                     TNEEYLQFIRSSRYIPSDTANYLRHWERGLPVSGQERYPVVWVSLEDARAYARWAGKR
                     LPAEAEWQLAAQGTDGREWPWGSEFHATKCNNGFGRPTPVDAFPKGESPYGVADLVGN
                     VWQMTDDVWCNGAYYFNIIRGGSWFRPESSWWYIKGGPQPLTTTQMQLLVSPGYDRSA
                     TVGFRCIKDL"
     gene            12538..12744
                     /locus_tag="PKK03_03295"
     CDS             12538..12744
                     /locus_tag="PKK03_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83450.1"
                     /translation="MKKVNCKAITLIILILSAILLMPGCATSKKNSYKSARKSSYIDT
                     SQLGRNKYFFSKKYQKKLYRFKKK"
     gene            complement(12816..13583)
                     /locus_tag="PKK03_03300"
     CDS             complement(12816..13583)
                     /locus_tag="PKK03_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX83451.1"
                     /translation="MKITFLGTGTSQGVPVIACECSVCTSPDPKDKRLRASLLVETAL
                     NTFVIDAGPDFRQQMLNARVKKLDAILLTHEHKDHIAGMDDLRAFNYKSQAAIDIYAE
                     ERVQTAVRKEYAYVFSENHYPGVPKMELHNIDNYNLTIRGDTFTPLRIFHYRLPIFGF
                     RIGNMAYITDANYIPEETKELLIGVKYLVINALRKEKHISHFSLGEALDLVREVSPRK
                     AYITHIGHQMGRHAEVSRELPVNVTLAWDGLEVNCED"
     gene            13766..14467
                     /locus_tag="PKK03_03305"
     CDS             13766..14467
                     /locus_tag="PKK03_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNX83452.1"
                     /translation="MSGTVYMIPVTLGDADPNLSIPIGTRDITLSIRLFAVEEARTAR
                     RWLKSLDRTFPIDSTLFFPVGKHSDVAGLEDFFVRVSGGSDAGVMSEAGMPGLADPGN
                     IIASEAHRRGIRVVPLTGPSSVMLALVASGLNGQSFSFHGYLPVDPQGRRKAVRELER
                     LSAGGSTQIFMETPFRNRKMMEDILAVCQPSTRLCIAADISLPTEFIRTMSVAEWRRQ
                     VPALEKRPAVFLIQA"
     gene            14684..17761
                     /locus_tag="PKK03_03310"
     CDS             14684..17761
                     /locus_tag="PKK03_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83453.1"
                     /translation="MIRRTILSAVMIMIAALVAGQKTDMSVFNGIKPRNIGPGGMSGR
                     VTAFAVDPRNENVFYVGTASGGLWKTVNAGTTFTPIFDNEEVASIGALAIDPQRPDII
                     WAGTGEGNPRNSVTGGYGIYRSLDGGRTWKCMGLELTRYIHRIIIDPVNPDVIYAGAI
                     GNPWAPHTERGFYRSTDGGGTWNRLLFTNETSGVADMVMDPSNPGKIFVAMWDHQRWP
                     WFFRSSSAGSGLWLTRNGGNTFERVSGGLPEETGRIGLAIARSNPDYVYAYVESKPSA
                     IYRSTDGGVNWERRGERNIGGRPFYYAEIYVDPENENRVYTLFSGVNVSEDGALTFDR
                     RTAESVHLDHHAWYIDPLNPDRMLDGNDGGMGITYDRGVTWRHIENLPVGQFYHINVD
                     NEIPYNIYGGLQDNGSWRGPAYAWYGGPLINEMYDFLIGGDGFDAMPVPGDSRYCYAQ
                     SQGGSVRRIDVTTGWSKSIRPAAEAGERLRFNWNSALAQDPFDNNTIYFGSQFVHRSS
                     DRGDTWTKISSDLTTNDPEKLKQDQSGGITIDATGAENHCTILTISPSSLKQGLIWAG
                     TDDGMIQVTEDGGRNWKNTSQNIRGMPRNGWVAQVTASLHDPGEAFAVVNDYRQGDNA
                     AYLFLTRDYGRNWQRIIDDTDVWGYVLCFVQDPAEPKLMFAGTEYGLYVSFDQGDTWN
                     RWTSGYPTVSTYDMVIHPREHDLVIGTFGRAVWILDDIRPLRAVASSGRKLLSGPLTV
                     FDAPDAYMASTKNLPGYYFYGDAMYRGENREQGAMITFYTGSDTGRVSVRLADEAGNV
                     VRTMQVNAKKGFNRFIWHFDRDPLPQLEYPSGQAQQQDSRARFFRGSGGTVIPGIYTV
                     SLSSGDASSEAKVRVNHDPRMDAPDTDALKRNYERARVIGSRIESLNARLRPLAQVRE
                     GLAKSESLVKENPGFATAVAEEYKAVKEELARMDEAFGRRQEGLSGRIGGYRALLSAS
                     GSLTAQEEKAVADAEAAIDEGLKLADAFTAGSWARYVEKLRQVTLSGDAVVLK"
     gene            complement(17791..18321)
                     /locus_tag="PKK03_03315"
     CDS             complement(17791..18321)
                     /locus_tag="PKK03_03315"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNX83454.1"
                     /translation="METNSTMGKKFNAAALAILAVIIVACNPAKKYEEEERSLIQDYV
                     ATNNITVSPDANGIYYIENVAGTGELIQRGDSVGIRYTGNFLSGKKFDDNLEADTPFR
                     FRVGSPFIIEGWSMTLLKMKMGSKAKVIMPSKMAYGSMGFGTYDYYGNYYTIIPGYSP
                     LLFEMEVVELTRKTKK"
     gene            complement(18347..18850)
                     /locus_tag="PKK03_03320"
     CDS             complement(18347..18850)
                     /locus_tag="PKK03_03320"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNX83455.1"
                     /translation="MRLAAYIAVIAVIAAGCQNKPDSRSKASPRTEAELIELNRSLIT
                     QDRNTINDYISKSDRSFSETSTGLWYSILGHGTGPAVKTGDNVNFDYECTLLNGNPCY
                     SGTQTIRVGYTDAGSGVTEGLQMMQEGSDYLFIVPPYLAFGLTGDGAKIPGRAILIYR
                     IRIRDIK"
     gene            complement(18847..19887)
                     /locus_tag="PKK03_03325"
     CDS             complement(18847..19887)
                     /locus_tag="PKK03_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNX83456.1"
                     /translation="MPEFGKYTKELKKLIDDAGHILLICHINPDGDAIGAMLALRRWI
                     ISQGKDAAMISPNALQKFLLWMNDVDKVVDFHHNPAEGTKLINNADLIIMVDFNTTSR
                     MGKAEQLVISSGAKKVMIDHHPDPTDIADLTVSDVSRSSTSELVYFLVNGMEGSEFND
                     SDFITAVYVGIITDTGNFEHGYYTGDTMRTVGHLLDMGVDKEKIYNHIFSNFSPDRIR
                     LKGFALHSRMVVHPDLHTAYIWLTKEDLDRFRHVKGDTEGFANVLLTMNGIVLSVLFV
                     ERTGFVKMSLRSKGSFNANELSRKCFNGGGHRNAAGGEMKGTIHEVVNHFENTLPSFR
                     KELEEAAREMAK"
     gene            19971..20390
                     /gene="ndk"
                     /locus_tag="PKK03_03330"
     CDS             19971..20390
                     /gene="ndk"
                     /locus_tag="PKK03_03330"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002859435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNX83457.1"
                     /translation="MSNEITFSIIKPDALGSGNAGSIISRINEAGFRIVALKMLHMTR
                     EQAERFYEVHRERPFYPGLVEFMSSGPALVMVLRHADAVEEFRRLIGATDPARALPGT
                     IRADFGTDVRMNAVHGSDSPENALREASFFFSAIEIF"
     gene            20433..22181
                     /locus_tag="PKK03_03335"
     CDS             20433..22181
                     /locus_tag="PKK03_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83458.1"
                     /translation="MTATVSPDFSDKKTLEKYSSAYTLSDMEIFIFPELFYPLVLANI
                     MSPEIWRWRDDPWFRDMHRKNFISRANRIKQYIIDNYIFNLDLETWGLTDKERELDRF
                     SDFFDTELLKQSNALFGYEGDKYYFSIDIRRHFGLDKYESSAIPYWKTETVEAMTAFR
                     HREHYTTGAGECVSLAALYAAAMFIVGEVPLEKIFMMATPLHSQNFIDEKEGLLTNNR
                     RIMTKNMWFNGTSLSGKARRALENEKVTIVSHITGHIHTVYERATIDRDAYNTFTKKL
                     RSFVKSPLTPAIFINFLRFKSEYKCLFQYHYLRTGTSHYITLDKLFEYEHSLKASMNE
                     ETRDKLLAEVDSEEFQYDPVPGKIMLNEVEAFIKQRRDSDLQVIEVEFTDSFRTEHTD
                     CVRRMFADIREFIITDPRLPSADREFVPEIHPQISVNDSRDEIISKIRGLAGVSEMAL
                     LTLYAYRDMEMADWRPFVKAAIERNPVCHSDLGGRNADEIYALINKLTNQSIYDSGRL
                     AQPDEVWNFGRGDGAEKAFLMADALVFNDPGAVVKILLEDDQAVVEYNYRYFRFATVK
                     GLRKKITISRKEYTVW"
     gene            complement(22253..22543)
                     /locus_tag="PKK03_03340"
     CDS             complement(22253..22543)
                     /locus_tag="PKK03_03340"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNX83459.1"
                     /translation="MRRNKTITLGEALGDLIKEYRLEKGLKEAAVLNIWENIAGKVIT
                     ARTKRTYLKDGVLHIYLTSSVVRNELMMLRDALRSQINSRAGEEVVREIMLH"
     gene            complement(22548..23663)
                     /locus_tag="PKK03_03345"
     CDS             complement(22548..23663)
                     /locus_tag="PKK03_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNX83460.1"
                     /translation="MMHLARLELTNFKNYEGVSLEFSQGFNCFVGNNGVGKTNILDAI
                     HYLSLTKSFFNSSDTLSIRHGDDYFMIRGTFVTDENSDELFCAFQKTKQKVFKLNGRE
                     YQRMSDHVGKYPVVMLSPADSTLITGGSEERRRFLNLIISQYDPSYLEAHMRYNKALM
                     QRNRILRGSGPDNSGLLEIYDEQMAPEAELIHSARRNLTENLLPLFRSYYEKISGGAE
                     KVSIRYRSHLGSGDFRQQLADSRVRDYAMQFTTMGIHRDDLVFEIDGHSARTNASQGQ
                     QKSFIVALKLAKFGLITRMIGFAPALLLDDIFDKFDQTRVEEIIRLAGSGEFGQVFIT
                     DTQQDRIHRILDNTGVDFRLYRIGKKGIEEEITNGVK"
     gene            23844..24524
                     /locus_tag="PKK03_03350"
     CDS             23844..24524
                     /locus_tag="PKK03_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX83461.1"
                     /translation="MSKKKETTKGIENVEQTLTKTEQFLEQNYKPLLYVLAGAVVLVG
                     IVWLLRMYTSKKNDEALSQMFMAEQYFGQDSLSLALNGDGNNLGFIDIAKEYKATRSG
                     KLANFYAGACLMHLGQYEDAISYLNKYKQDDEVIAPQAKGLIGDARVELGDMAAGIKN
                     YLEAADMAENAFLTPIYLMKAGMLYESDGKWADALKLYERIQDKYPESNEGRSIDKYI
                     ARVKLHID"
     gene            24528..25016
                     /gene="ribH"
                     /locus_tag="PKK03_03355"
     CDS             24528..25016
                     /gene="ribH"
                     /locus_tag="PKK03_03355"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNX83462.1"
                     /translation="MATRDLSDYDPSRVPDASGMRFGVVVADWNSEVTHPLLDGAVKT
                     LKRHGVTADNIIVRHVPGTFELTLGAQWMAEYEDLDGVICLGCVIQGETPHFTYICQG
                     VTQGITQLNLDYNIPFIFGVLTTLDLRQALDRAGGKHGNKGDEAAVTAIKMAALQGLM
                     EK"
     gene            complement(25094..28030)
                     /locus_tag="PKK03_03360"
     CDS             complement(25094..28030)
                     /locus_tag="PKK03_03360"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNX83463.1"
                     /translation="MDFLEKVAYRLRERHGNEMEKQTVVLPSRRAGLWLARALARMND
                     KPSWAPSMLTVSDLFRSFTDLIPADTETQIFELYRIYRNLFGEEISFDDFWPWGEVII
                     NDFNDIDLYMADAGKLYSNISDLKEIDVKFGGLTDEQVEIIRGFWKSFNPSSAGSEAR
                     SRFRSVWQRLGPLYEGYREAMRARGLAGDGMLCREVAGRAQSGLLEVPEGRRWHIAGL
                     NALNNCEKTLFMHLKRLGVAEFYWDDDHFFMDDPEHKASLFIRENRSIFGNSLSGEGA
                     GSGTGSGSGTGPGTEIGSGASSGRHQTPRGEWHIIDTPSDTAQAAMMAQLLENEGITG
                     GDDLTSTAVILADEKLLMPVLGSLPASVEDVNVTMGHPFRFTTLYSFLRQLMALIRSA
                     RVSNGILSFRSDDVMALLRHQYIRLLGGNEGDTVVKEIIKGNMIRVTAAMLAERFRLD
                     YLFRVPEGGAGIPDHLASVMARLEEATYAAEEQGMKLSTDREYLRMAMGSTVRLSNLI
                     RSYDLDLMPETCLRLLDRLFRKMIVPFSGEPLRGIQVMGVLETRALEFRNIIILSLNE
                     GIFPGMSYDNTYIPYNIRRAFGLPTVNEHESIYSYYFFRLLRKPEKGWFLYNSTAQGL
                     NSGEMSRYLVRMSYNPLFAPRHRSVHISVGRSGMIAETLAKTAAHNRTLLDIYGEGNR
                     DNKYLSPSAVNTWLNCRMRFYYRYVCGMPEEEVLEKEIDQRRFGNIMHDALRRMYQPL
                     RGKENAGSIIALMARSRENIRETIIAAAMDEMRWDRETLLSGRGVIIIDVLERYAREL
                     LQYDGANSDLVLLNLEDDFSGVRAVTCREGEKRIVLGGRVDRVDLTRGAIRVVDYKTG
                     APKREAVTAEGLFDEEKEKRNDALLQALLYCTLIENKHPGRMVLPAIYWIQQLTSDDF
                     SPYAPVPGLDGPESSMNEWNEFMTSFGSGLDMTLNRIFAEDEDYRMTTFRRRCISCPY
                     RTLCRR"
     gene            complement(28033..28992)
                     /locus_tag="PKK03_03365"
     CDS             complement(28033..28992)
                     /locus_tag="PKK03_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133901.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HNX83464.1"
                     /translation="MNGEQNYVWGTARRYNSYADWFAGLFGGRVQKLSINAGFTCPNR
                     DGTLGYGGCTYCNNEAFIPGYCTPEKSVTDQIEQGIAFHRRRYRRAERYIAYFQAYTN
                     TYADIDKLTALYDEALNHPSVAGLVIGTRPDCINGELLDRLAVMARDYFVMIEYGIES
                     VNDDILRRINRGHDFTTAMKALKETELRGLNAGAHLIFGLPGESRSAIADSAEVISSL
                     PLTSLKIRQLQLIKGTAMAMEYRKNPGAFDLYDLEEYLDLVITFTERLSPGIMIDRIS
                     GEAPPRLLDDPRDWHLRSSELFRLFEHRLEERNTWQGRLCKMI"
     gene            complement(29042..32272)
                     /locus_tag="PKK03_03370"
     CDS             complement(29042..32272)
                     /locus_tag="PKK03_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNX83465.1"
                     /translation="MKLPGKLLKYSASAGSGKTHALTAFYLSQVLHDPGAYRRILAVT
                     FTNSAAAEMKERILRRLNTLGLENEKGDRERKEFAVWLCKFFPEMWPDREAAVVTIRR
                     NAPVALDNILQDYSRFAVGTIDSFFQRVIRAFAREIDIPAGYEIELEHQAILSDAVDS
                     LLAEVATDGKLLDWISSYVASRLDDSKGWDIRREIMEVAAQVFREDFRQLSTDDRKMI
                     GDYGVMADYASRVFAVRRGFETDLKNLAAEGVKIYTECGLSTDDFLSKSRGGVGESLR
                     RFAQGDTRRPNSTWVKAAAEGKFFPGTARPAVVSAFGDASRRGLDDVVKKILDLFDRR
                     YGVYVSALAQVRTIHVIGILGAISERVREQAHEQNIFLLSDSGELISKLIADDDTPFI
                     YEKIGAAYDHYIIDEFQDTSRIQWNNFRPLIAETLSRGQDNLVVGDVKQSIYRWRNSD
                     WRIIHSEAAQVFGDAVETVHLSTNYRSRANVIRFNNALFSPASIPASCDERLAYEGLK
                     ISDVYSDSEQQGTPGKPGGYVSVTLYRKNDDEGWKDRVLRDLPGVIERLQEHGYRADD
                     ILFLCRTNEEGKSIISRILEYSSLCTPGQRARYNYEITSGESLLLERNPAVTLMTASL
                     RYLTDPASRINRSQIVRSYILATGGDERLVYSGDTPDGGGESLPEGWEEAIESFRNAS
                     LYSATEGMIRFFGLGRNRANTAYISSFQDVVLAWSGRHSSDISAFVQWWDDEGCKSTL
                     SQSERQEAMRVMTVHKAKGLQSRVVIVPFASWEFSKPGYARSLLWVTNVPAPFAPMPV
                     VLPEMSGRLDESLFSGEAKMERASDWLDGVNLLYVAFTRAVDALLVMTQEVTRGPAGG
                     SGANTASLLNEALAKLPGDFKTADEETKRHIECGELPAVETKEREPWLEISGYVVSEP
                     RGTLRLRTGGALPADEVKLAEPGGRAYGIMMHEILSRVTTTADIEKAVSHACATGLMP
                     LTGRDTAVARLHAMLSAERVKAWFDGTTAVMTEATIILPTGAARRPDRVMISGDRVTV
                     VDYKFGEPSPRHKQQAADYRELLRQMGFPEVKSWLWYVEKDLIEEA"
     gene            32362..33582
                     /gene="mnmA"
                     /locus_tag="PKK03_03375"
     CDS             32362..33582
                     /gene="mnmA"
                     /locus_tag="PKK03_03375"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNX83466.1"
                     /translation="MKKKAIVGLSGGVDSSVAAWLLKEQGYEVEGIFMINWHDSRGLL
                     KGDCPFDEDITFAEMVAKKLGIRLRTVDLSVEYRERVVDYMFREYAAGRTPNPDVLCN
                     REIKFDSFAREALKQGADYIATGHYCRNEQASTAGGMVNRLLAGVDSNKDQSYFLCQL
                     SQGQLDNVLFPVGSLTKPEVRRIATDLGLATAVRKDSQGICFVGKVDLPVFLQQQLEA
                     RKGRIIYVPDSEAFRSWPDGLPQEGVTTAELSEMTIPHSLSPGDGVTAGVHNGAHFFT
                     VGQRKGLNLGGYREPLFVIGTDTVNNIIYVGEGQLHRGLYRKGLFIRKTEVHWIREDL
                     AMNPGEDRDYQLRIRYRQPLQHARIHARDEGYYIIFEKSQRGITPGQFAAWYDGEELL
                     GSGPIDEQSGIIGC"
     gene            33670..34164
                     /locus_tag="PKK03_03380"
     CDS             33670..34164
                     /locus_tag="PKK03_03380"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HNX83467.1"
                     /translation="MLSLVFVLLAFGLNAQSNVIKTNPLGLAFGSFNATYERVFGTTS
                     SLLVRGQYMYKLLGVDVSLAGAGLGYRYYFTHAKKPVPAGFYINPQLAFSFGKVNDDT
                     EGNYSASTIGFGAELGYQWVWESGFVLDLGIGPMYTIAKGGDSESSIDGIMPSATLAI
                     GYAF"
     gene            complement(34328..35425)
                     /gene="nspC"
                     /locus_tag="PKK03_03385"
     CDS             complement(34328..35425)
                     /gene="nspC"
                     /locus_tag="PKK03_03385"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HNX83468.1"
                     /translation="MEEERLRKNLSLIKHVADASGAEIILAFKGFAMWSVFPVVREYV
                     SGAAASSIHEARLCFEEMGTPAHTYAPAFRRDEFGTILRYSSHITFNSLEQYRKFAPA
                     LHREGKHISPGLRINPEYSEISQGIYNPCSPGSRLGVTAAELANGLPEGVEGLHFHVL
                     FESDSYTLEKVLAVVEEKFGHLLPKLKWLNMGGGHLMTRRDYNTGHLIGLLKKFREKH
                     RIHLILEPGSAFAWETGELVATVEDIITRDGIKTAIMDVSFTAHMPDCLEMPYKPVII
                     GAFDPVPGKPTYRMGGNSCLSGDFMGDWSFHRELHPGDRLIFWDMIHYTMVKTTTFNG
                     VPHPNIGIWTADQQFRLVREFGYADYRDRLS"
BASE COUNT         8497 a         9058 c         9362 g         8510 t
ORIGIN      
        1 atgaagagaa ctggggtctg aagaaactcg cttatccaat ccagaagaaa tcgacaggtt
       61 tttattacct gatcgagttc agagggcagg gcgagttgat tgacaagctg gaagtacagt
      121 acaggaggga tgagagaatc atccgtttcc ttaccttccg tatggacaag tatgctgtag
      181 agtatgccga gaagaagaga aagatgaaag aaaccgaaaa agtcagggag gagtaatcat
      241 ggcacagaac aattcagaaa tcaggtacct gacaccgcct acagttgaga tcaagaagaa
      301 gaaatactgc aggttcaaga agaacaagat caagtacatc gactacaagg atcccgagtt
      361 tctcaagaaa tttctcaacg agcagggtaa gatacttccc cgccgcatca ccggcacctc
      421 gctgaaatat cagaggaggg tcgccaaggc aatcaagcgt tcgcgtcatc ttgccatgct
      481 tccgtatgta gctgatcttt taaagtaagg aggaagagat atgaagatta ttctcaggca
      541 ggatatagag aagctgggac agaagaacga cattcttacc gtcaagaccg gctatgcaag
      601 gaattacctt attcccaagg gtttagcagt acaagcaact gactctgcgc tgaagattca
      661 caatgagaat cttaagcaga gggcccacaa ggaggaaaag atcaggggtg aagctgagaa
      721 gcttgcacag aagcttgctg gagtgaagct cgtgatcggc gccaaggtca gctcggccgg
      781 caagatcttc ggatcagtaa ataccattca gctggctgaa gccctcaatg caaagggata
      841 cgagattgac cgcaggaaca ttctccttgg cgaggatgcc atcaaggaag tagggagcta
      901 caaggcagtt gtcaaactgc accgtgatgt aaaggtggat cttgaattcg aggttgtggc
      961 cgaataatta ccacacacca tattgatatg catacggcgg ggggtctgag accccccgct
     1021 gttgcatatg gatacctatc tgcctggcat gcaggttgat catttgactg ccaccgtctc
     1081 tatctcgatg agggcgccaa gcggcagacc cttgacggca aatgctgccc tggcaggctg
     1141 gtcagctgta tagaactgcg aataaacctc attcattgcc ttgaaatcag acatgtctga
     1201 caacaggcag gttgatttga cgacatcatt catcgtatag cctgctgctg tcagtatagc
     1261 cctgatgttt ttcagcgcct gggtggtctg ttcggtaatg cccccctcaa ccatctttcc
     1321 cgtggcagga tcaatgggta cctgtccgga gatatagaga gtgccgccgg cctccacagc
     1381 ctggctgtaa ggtcctattg cccccggcgc accggttgtg ctgatgattc ttttcatacc
     1441 tgatatcttt gattcgttag taattctcat ggtaatcctt cctcctttcg tacttgagat
     1501 cctgaagcat gcttgccttt gccctgatgg tgaagttcca gctcttcatg taaccggtgg
     1561 ggatccatga aaagctcatc tcccagcaat ggaggtcacg gctgataccc accctcgtca
     1621 tggttatctc cttctgtttg aagtcgtatc cggtattgta gttgagagcc atcttcgggg
     1681 tcagcttcag gtctcctgac agggtcatcg cctgggaaat gctgggacgc agaccaggtt
     1741 tgctgtagct gaagttatat gccattctga gcgaccacgg cacgtcgaac ctgacatatc
     1801 cgaactcgtc caggtttctg ttcgagagtg gaaggttgcc cggggcagct ccgccctgag
     1861 ctccgggatc gccgggctgg cggcccgcgg acatttgtcc cccctgcccc ccggactgtt
     1921 gttttttgtc cttgttcccc agcaactgcc ccaggtcaag gtcaaaactc atgttgaatc
     1981 ctgtcatcct ggcaagaccg agtccctggc tgatggcaag ctcgttaact gcgcctcctg
     2041 tggcgttcat gccatagatc gaaaaactgc cgtttgcctg gatgtttatg ttctgtgcca
     2101 gggttgtccg gaaggatacg ctgacaggcg accagttcag ggagtcggag aacaagttgt
     2161 atgaggtgtt aagcgacagg ttatcaatca gtttaatctt cctgggcttg ccggtggtat
     2221 cattcctgga atagaccttg gcttctacga tatttgaaag gctgaaggcg acagatcctg
     2281 acctggtacc cgttgacggg gtcccgaaga tgcttccttc atagatggaa tactcgcgcg
     2341 tcctgccaac agtgtcgtat tgaactgtcc tgtacatgtc actcgaaagc ttatctgtct
     2401 caggcgaata ggaaaagctg acggagggct tcattacatg ccttattgac tcaagccttg
     2461 atcccttttt tgtgaacgaa aaagtaccgt agacggaagg attgaatcct gcactcactg
     2521 tcggcaccag tgcatggcca taggtgagtc ccctgagggt gtcattcaca accgagggga
     2581 caaccatgtt cctggtttca tcatagtaat cagggtccca tctcttcgta atctgctggg
     2641 tgtagagcac tccagtgtac ctaagcgacg gggagatgga gaagttattg aaaggtctga
     2701 actgaaggct caagggtacc tcatgcttga agccgttctt catgtttttc cacaccgcac
     2761 tggtgaacag gaggctgtca tatgtgtcta tcttattctc aaatgaggcg gtatactggg
     2821 ttgttatatc atgataccac ctggtcttcc ccactggctt tttaggcttg aacgggtaga
     2881 tgcgcgagac gttcaggctt ccccttggca ggttgagcat catcgtcttg ttctgcacgt
     2941 tctgcgactg gttgatgctc gtggaaaagc ttacaggtgc tcctgcccat gattttgtat
     3001 agctgatgct tgactgtctg gtggtggtaa catggtctgc cacttcatag ctgttgttcc
     3061 tgtcatagcc gttggagctc atattcacgc tggcggagaa ccgagaaccg ggattggcct
     3121 ttgtatcctg cgaatgcgac cagctgatgc ggtagttggt gttcttcccg taatcgggaa
     3181 gccccttata gctggtaacg ttattggcgt aactgaaacc gaatgatccc gagtagcggt
     3241 accggagacg gtatgaggtt gctgcatcgg caagccaggt gccgttggtg tagatagtgc
     3301 ccgtcagttt cagatcgaag taatcgctta acgcgaaata gtaccccccg tttgaaagga
     3361 aatatcccct ccgggcctcc tggccgtatt tgggcatgac gattcctgag gctctctttt
     3421 gctgtaccgg gaagaatccg aagggcagga tgagagggag gggaatgtcg gctacgacca
     3481 tatatgctgg tcctgatacg atcttttctc ccgggtatac cttggctctc gggagggaga
     3541 gatagaaatg aggctcctcg gcctcacagg tggtgaactt gctgcggttg acgtgaaggg
     3601 tcccgtcctc gtgtctcttt gttgtcaggc tctgaaggaa gcctccctcc tgttcggtgg
     3661 tgacattacg gatcaccccc ttttttgatt tgaagttata gtttagctct ttcgattcga
     3721 actcctccga accatccttg tatactggca ggcctacagc cttgccggtg gaatccctcc
     3781 tgaaggtggc gtataccgat cctgtctcca tatccagcac aatggagtca gccgtgagtt
     3841 ctatggttcc ataggtgact ttcgcattct ccaccaggct gactttcttc gaccgcatgt
     3901 cagtcttcat gtaaccctca gaggtataga caattggttc atcaacagca tccggagaaa
     3961 taataaggcg tgttttagtc agcgaggtat ccgtgattgt ggtgaattcg ccgggaaaga
     4021 gtgtgtcatt tacaaatgca ggagaaggat tcacttcatt cagcccggtc acagtggtat
     4081 caggaaggga cttcagaacg gtgtccggga ggttcttccc ggccgtgaca ggctgcgccg
     4141 gctcctgggc aagcaatgag gcctggaaga gaagcggcat gaaaattgcc acaaacaatc
     4201 tttgtggaaa agaatgcaag ttaaatgcta tttttgcgta attaataagc atctctgaag
     4261 agccagcgac aaaactaatt aaaattcgca aagcagtaat gcggcaaagt accaaaataa
     4321 gagggttaag agttattccg gcatgtgccg tatttatagc agcaataagt atgccggtga
     4381 atattaagtc acagccgctc ccgtctgaag atgagtgggt agtggtcatt gatgccggtc
     4441 atggagggag ggatcccgga gccgtcgggg caaaggcaaa ggagaagaac atcaaccttg
     4501 ccgtagcgct caagacaggc aggtacatca gtgacaacat gaaggatgtg aaggtcatat
     4561 acactcgtga cgatgatact ttcctcgggc tggacgagag ggctgaggtt gccaacagga
     4621 acaaggcaga cctttttatt tcgattcatt cgaatgcaat gcctgacaaa cggttcaccg
     4681 gggctgaaac ctatgttctt ggtcagacga tggacgaggc caacctgcag gttgcaatga
     4741 aggaaaactc agttatcacg ttagaaaagg attacgaaac gaagtacgaa ggtttcgatc
     4801 cgaactcggt ggagtcgtac atcattttct cactcatgca gaacacctac ctcaggcaga
     4861 gcaccgaatt cgccacaatg atacaaagcc agttcagaga cagggttggc aggaaggaca
     4921 ggggtgtcag acaggcaggt tttcaggtac tctggagaac aacgatgcct tcagtgctgg
     4981 tggaaatggg cttcattacc aatgccgagg aagagaagtt cctcgtatca gagcagggac
     5041 aggattatct tgcctctgca attttcaggg cattccgtga ctataagcag accattgaca
     5101 gcaggacagg agtgagaaac ggtaaagttg ttactcaggc agcggctccg gtggcgggca
     5161 atgaagactc cacggccgga aaagggacgc aggtgccatc aggtcagcag gaggccagac
     5221 ctgcggtatc agcggcaggg aatgaagata aagctgtgga tcagccggtc aggagtgaca
     5281 ttgtaccttc ggaaacggct gtgaagaatg acagtggtgc ggcagcggca aagcctgttc
     5341 tgatctctcc tgccccggca gacgatattt attttatggt gcaggtatct gcgatgccga
     5401 aaaacagaga gctgacacag agccagttag aagggcttga cccgataagc aggattgaag
     5461 acggcgaaag aattaaatat gcatctggaa aattcgtttt atatgatgac gcgttgaagc
     5521 acaggcgcac gcttacggga agataccccg acgcattcgt cattgctgtc agaaacggaa
     5581 agattatgcc tctgagggag gcgatagaac gacaaaagag ataaaagcaa ccctatgaag
     5641 atatcccgcg aggtaaaggt cggcgccgta gcactgatca ccattattgc attcatcctt
     5701 atgttcgagt ttctcaaggg cactgccctg ttcacaagta cagacactta tcatattgtg
     5761 tatgataaca ttgccgggct tacagagtca aatccggtcg agatcaacgg ttaccaggcc
     5821 ggtgtcgtcc aggatgtgaa gctggtcaat gacgggtcag gacgaatcct ggtatcactc
     5881 tctgttgaca agcacttcaa catacccagg gacacagagg ctgagattac cacagccacc
     5941 ctcattgccg gcatgaagat agtcctgcgc atgggagaga gcagcgaaat gtgccataac
     6001 catgatacaa tatcagggta tgtggccaca tcaatactag acaggctgag tgagaccatc
     6061 agcccgctgg aaggcaacat caccagtata attaccaggc ttgactcggt ggtgtctgcc
     6121 ctcaacagcg tctttactcc cgaaatgacc ggtgatatcc gttccactat ggcaaacatg
     6181 agtgatgtta ccgccgggct gaagcagatt tctgacagca ggaaggatga gcttatggcc
     6241 gccatcagtg agatccaggc cttcaccgcg atgctttcat ccaacagttc aacaattgat
     6301 tccaccctta agaatataag cgggatctcc gaagctgttg ccagggctga cctggagacc
     6361 tctctggcct ccctccgctc atcactgaac ggacttgaca ccatcgtgac cggaatcagc
     6421 cggggagaag gctcggcagg aaagcttgtc acaagtgaca gtctctacac aaacctgagc
     6481 aattcactca ggagtcttga cctcttactt cgtgacatga aggaaaatcc gaagaaatat
     6541 gtccatttct ccatttttgg cagaaaatcg gaatgatttg ttaaaataat actaaacaat
     6601 acaatatcac cgtgttgcaa gccaaatgag gggaatttgc cttaacaggg catttcagga
     6661 tatagatatc ttcaatctgt aaaaggctgt gtaacatctc attcatgtta atgtactgat
     6721 tatttggaaa ttagacattt tgaccaatgt ttgccaattt aagggactga ggttcatcac
     6781 agtatagaat aaatggcaaa gtcaatagta aaaaattttt ttttttacct ttttttttac
     6841 aaatattgtc ccgggtttca gcaccagaaa agaaagctac tcctttttaa tttctatctg
     6901 aatgaacaag tcacacgatg aggtttggaa taattgtctt gagataatcc gtgacattat
     6961 tccttcggcc agctttaaga cctggttcga gcctattgtt ccgctcaggc tcgagaaaaa
     7021 catactgaca atacaggtgc caagcccttt cttttacgaa tatctcgaag agcagtacat
     7081 agacatcctc cgcaagaccc tgaggcgtga gattggcaat gatgcgaagc tcgagtacaa
     7141 cataatcatg gagaattccg gctacgccaa cggcaagcct tacacggtaa ggtatccgtc
     7201 aaggaacaag gctgatctcc agaacaaacc gatgcaggtt ccctttgagg tcacccagcc
     7261 tgcaataagg aatccgttca ttataccggg gatcaagaag cttcattttg atccgaggct
     7321 caatcccgac tattcattca acaattttgt cgagggtgaa tgcaaccgtc ttgcccgttc
     7381 agcaggcata gccgtaggga acaatccggg aggcaccgcc ttcaatccgc ttatgatcta
     7441 tggtgattcc ggccttggca agactcacct ggtacaggcc atcggcatcc tggttaagga
     7501 gaagtatcct gacaagaccg ttctgtatgt aaatgccaac aagttccaga cccagtttgt
     7561 ggagtcaata aggaacaaca acaagaacga ctttctccat ttttaccaga tgattgacgt
     7621 tctgattatt gatgacgtcc acgagtttgc cggcaaggag aagacgcagg acacattctt
     7681 ccacatattc aaccacctcc accagtcggg caagcagctg atactgacct gtgacaaacc
     7741 gcctgttgag cttcagggga tggagcagcg tcttctctcg cgtttcaaat gggggctgct
     7801 ggccgatctc cagaagcctg attttgagac aaggctggcg atactgagga agaaggcgta
     7861 caatgacggc attgagctgc cggacgagat tttcgagttt ctcgcagaaa acatctcatc
     7921 aaacatacgt gagctagagt cagggcttat ctcactctat gcccagtcga ccctcaaccg
     7981 caaggaggtg acactcgagc ttgcgcgcca gatggttgac aggctggtga acacccagcg
     8041 cagggagatc acaattgatt acatccagaa ggttgtctgt gagtattaca agattccggt
     8101 tgacctgatg cagggcaaga cccgcaagag ggagattgtc caggccaggc aggtatcgat
     8161 gtatttttca aagaacctga ccaaagcatc gcttgccagc attggttcat ccatcggagg
     8221 gaaggatcac gccacagtgc ttcatgcctg caagactgtg aacaacctca ttgacaccga
     8281 ccgccatttc cgtaaccaga taatggaaat agagaaaaaa tttaaagtat gaaatcagac
     8341 agtgacagac tgaaggaaga gtttttcagg ttatacgggg acagggacaa agagcctgtc
     8401 cttttttatt cacccggcag ggttaacctc attggcgagc atactgacta taacggcgga
     8461 tatgtatttc cgtgtgctct tgatttcggg accaccctcc ttgtgcggcc cggcagcgga
     8521 caggagatca ggatgaacag tctcaacttc ccgggagagg tggtgattcc cctggctctc
     8581 aattatatgc ctattccgaa gagctggacc aactaccccg tcggggtcat caacgaattc
     8641 gcccgcaagg gcatcggggc tccggcaatg gagatgcttt tcacgggaga cataccaaac
     8701 ggtgcaggac tctcttcttc agcatcgatt gagatggtga cggctgtggc catgaatgag
     8761 ctcaccggcg ccgggctgga gatgatggag atggtgaaga tgggccagaa ggccgaaaac
     8821 ggcttcgtgg ggatgaactg cggcatcatg gaccagtttg cggtgggttt cggcagggcc
     8881 aaccacgcca tcaatctaaa ctgcgacact ctggagtacc ggtatgcacc tgtcaacctg
     8941 ggtgacagga agctggtcat cacgaatacc aacaaacgaa gggggctgac tgattccaag
     9001 tacaacgaac ggcgggccga atgcgaaaag gctgtggcgc ttatctcatt gcacaggccg
     9061 gttcgtaacc tgagtgagct gaccgtcagt gacctgtgga tgcctgacag ttacattcct
     9121 gaccccgtcg tcagaaagag ggcaaaacac gtcatcactg aaaacgggcg aacactcgat
     9181 gcaataaagg ctctggagaa caatgacatt gagcttctcg ggatactgat gaatgagtct
     9241 catgactcac tctgccgcga ttacgaggtt acgggcgccg agcttgacac cctcgtttac
     9301 gaaggaagga agcttccggg cgtggtgggt accaggatga ccggtgcggg attcggcgga
     9361 tgcacggtat caatagtgga ggcggaccac gttgacgaat tcatcagcag gctgggtgag
     9421 atatacactg caaagacagg cctcaccgca gacttttacc tgccggggat cggtgacgga
     9481 gccaggagaa tctaagaatt gcgattagcg aattgggagt tgagagttga gagttgagac
     9541 tgccgattga atttgaaatt cgcaaatcgc aaatctcaaa tcacaaatca cagatccttg
     9601 atgcatctga atccgacggt ggcgctgcgg tcataaccgg gtgaaacgag gagctgcatc
     9661 tgggttgttg tcagcggctg cggtccgccc tttatatacc accagcttga ttccggcctg
     9721 aaccagcttc ctcccctgat gatattgaag taatacgctc cgttgcacca tacatcgtct
     9781 gtcatctgcc aaacgttacc gaccaggtca gccactccgt aaggactctc ccctttcggg
     9841 aaggcatcga ccggggtcgg cctgccaaag ccgttattgc atttggtggc atgaaactcg
     9901 cttccccatg gccactcccg gccgtcggtg ccctgtgccg ccagttgcca ctctgcttct
     9961 gccggcagac gcttaccggc ccaccgggca tatgccctgg catcctcgag acttacccag
    10021 acaacaggat agcgctcctg tccgctgaca gggagtcccc tctcccagtg cctcaggtaa
    10081 ttggcagtgt cggaaggaat gtacctcgat gatctgatga attgcaggta ttcctcgtta
    10141 gttaccggga acctgtcaat caggaaactg tcaacagcgg aggtgatctc cttcccggtg
    10201 cggggatagg gaacaaactc atcgacagcc ttcagattat atcgtattgt ggccgcgggg
    10261 acaagcacca tcccggccgg gatctcaggt gcaggaaggg tgcgaaaagt attagagaca
    10321 agccatggcc tgccctgttc cggccttatc actctctcat caagcaatat tccttcgctg
    10381 tctgtcagct cgatcactat cttgtcgccg gcaattccca ggagggcatc ggcatccacc
    10441 agcgtatcag caggggcggt tatcctcagg ggctcatttg tataacccgg atctcccctg
    10501 cttaccagca gcattccgcc cgcggaagag gctatccgga ttctgctgcc atgaaactca
    10561 gcagtgatca gtcgtggcag caatgccaca cagtcaattg agctctcgcg ccttgttccg
    10621 ctgaatgcct ggctccaccc gggaagatcg accggggcag tgagagtgtt gttgattacc
    10681 gagggggtca gctcctcatg acggaagagt gataccaggt gtttgccggt ggtatcgccg
    10741 gggctgaaca gagggccctt gtatccgcga tgatccatgt tcaggacggt aaacaccttt
    10801 ttctcaccgg cattccagct gttgacataa accctgtcgg ctccgcttcc tatcagtggt
    10861 gtccagttgc cgccggcaaa ggcatcactg tttctcctga gcaaatatgt tgtctgagca
    10921 aggtaatcaa ggtctttccg gaactcatcg cccctgccgc ccggcctgaa catgttaagt
    10981 tcagtgccgt agccgttgaa gaaggcaacc gatatctcac ggtgaagcac atcctcgcca
    11041 acatctgcca cccggaagat ggcaaaatcg ggtctgatca ccttgttaag attaagttca
    11101 ggactgagat acagggcatt atgaaccctt gcagccacga tgccgggcat atccctgacg
    11161 acaggcattc cctcggagta catcaccaca ccgctgcgaa cggtatcggc agcctgttgc
    11221 agttcagatg aagagcggcc ctcggtatca agcaccacgc cgtctgcctc tgtttctgca
    11281 attatctgtg tcattccgtg gaggtgatcc tcctcgcggg tgcttttatc ccacggattg
    11341 tatgcaatga aaaagcggca tccctgtgac cgtgcctccc tgctgaggcg cctgagagcg
    11401 tcggttcctc ccggcaggtc acgatagagg tcccactggt tcctgtcgtc aaggccgagg
    11461 cgcggccatg tcggccagat gccgtataca tcgaggtgac cgaacagcct gttgtattca
    11521 gccaggtagc cttcaaacgc ctcgttgccg gtgaagcggt cacgcagttc cctgtcccat
    11581 gccatctgga gaatcatcag gtagctggtt cgtatccatt tcaggtcttc cctgtcatac
    11641 agggtttcgt caaaattttc cagatcatgc agccaccgtc cggcgaacat cagccttaga
    11701 ccctcctgcc attcaccctt atacagatct gcatggatgt ggtaggtcac ggatgctccc
    11761 ggaggaagta tggtttcata gcgcctgata atgccgttgt cggttctgat ccgcctggca
    11821 atggaagcca acgagagtcc cggtgctgca tcgaaggtgc tgaatcccat ctcccatgcg
    11881 ttgtcaggga ggattaccct gacgggactc ttcccgggca gaaacaggtt tgcccttgcc
    11941 aggtcacgag gtccggcccc agtgataaat gccttgtttt ccttttcgcc gaatggcacc
    12001 acgttggtca cggtgatggt gtcattccag ttattgcgca gcacaagctc ggccatgaat
    12061 cccggatgaa atccgccccg gggagtataa ccggcctcgg cacctcccgg ataaatcatc
    12121 aggaaacggc ttgcgacgag cccggcaggc acatcgtccg attcgaaata tgtatcgtca
    12181 agcctgaagc tgaagagcgg acggggagtg tcaagattta ccccggaact gtcgtcaagg
    12241 atgagcgata ccgcgctggc gccattgtca cggatgattc caagattgat gagtgactgc
    12301 ccgttgagcg aaaggcagac aatgaaaaaa aagagggaaa accgaaacct aaagctgcac
    12361 ataggagttt aaactgatat aatttgcttc ccgatgttac gggaatgcaa tttatgatat
    12421 tgtatctgaa aataaaagcg aagatacagt gaatgaataa ttttagagat aatatttacc
    12481 cataaagtca cgttacagat ttatacctgc ttttagaaat tagttcagcg caatctgatg
    12541 aaaaaggtaa actgcaaagc catcactctc ataattctga tactgtcagc gattctgctg
    12601 atgcctggct gtgcaacctc aaagaagaac tcatacaagt ctgcaaggaa gagttcatac
    12661 attgacacct cacagcttgg gcgaaacaag tatttcttct caaagaagta ccagaaaaag
    12721 ctttacaggt tcaagaagaa ataggcttat ccaatcagga atcccgcctc cggtctgccc
    12781 cggcagtgat gcagggaaca gcagcaagca gagtcttaat cctcacaatt cacctcaagg
    12841 ccgtcccagg ccagtgtcac gttgaccgga agttccctgc tcacctcagc atgcctgccc
    12901 atctgatgac ctatatgcgt aatgtatgcc ttccggggtg atacttcccg caccaggtcg
    12961 agggcttcgc ccaggctgaa gtgagagata tgcttctctt ttctgagggc attgataacc
    13021 agatacttga cgcctataag tagttctttt gtctcttcag ggatatagtt ggcgtcagta
    13081 atgtatgcca tgttacctat cctgaaacca aatattggca ggcggtagtg aaatatccgc
    13141 agcggagtaa aggtgtctcc cctgattgtc aggttgtagt tgtcgatatt gtggagttcc
    13201 attttaggga cgcccgggta atggttttcc gaaaagacat aggcgtattc ttttctgact
    13261 gccgtctgca ctctctcctc agcatagatg tcaatggctg cctggctttt atagttgaaa
    13321 gcccggaggt catccatccc ggcgatatga tccttatgtt cgtgtgtaag aaggattgcg
    13381 tcaagttttt tcacccgggc gttaagcatc tgctgtctga agtcaggacc ggcgtcaatg
    13441 acgaatgtat tcagggcagt ctccaccagc agggaggctc taagtctctt gtccttcggg
    13501 tctggcgaag tgcagacgct acattcgcaa gctattaccg gaacgccctg ggaagtaccg
    13561 gtaccaagaa aggtgatttt cactatctca tttttctcac tgcaaacata acccgaaatt
    13621 gagagaacta caatatcagg catcactttg gaacaggagc cttcagctgc gggtccggcc
    13681 tgcctggatt gattcagtca tgatttcagg ggtttgtgaa aagagggtga ttttatgtaa
    13741 gtttacggta caatcagtca ttaagatgag cggaacagtc tatatgatac cggttaccct
    13801 gggtgatgcc gatccgaatc tctccattcc catcggcacg cgtgatatta cgctgtcaat
    13861 caggttgttt gccgtggagg aagcacggac ggcaaggaga tggctgaaga gccttgacag
    13921 gacttttccc attgacagca ctcttttctt cccggtcggc aaacactctg acgtggcagg
    13981 cctggaagat tttttcgtca gggtatccgg cggctctgat gcgggggtga tgagtgaggc
    14041 gggaatgccg ggcctggctg acccggggaa cataattgct tctgaggccc accgccgtgg
    14101 catcagggtt gtgccactca ccggtccttc atcggtgatg cttgcgctgg tggcatcagg
    14161 gctgaatggc cagtcgttct cgtttcatgg ttacctgcct gttgatccgc agggccgccg
    14221 caaggctgtc agagaactgg agaggctctc tgccggcggc agtacccaga tcttcatgga
    14281 gacaccattc aggaacagaa aaatgatgga ggatatcctt gcagtctgtc agccctcaac
    14341 caggctgtgc attgcggctg acatctccct cccgacagag ttcataagga ccatgagcgt
    14401 tgcagagtgg cgccggcagg taccagccct ggaaaagagg ccggcagttt tcctgataca
    14461 ggcatagaga gaagatcaat ctgcacacaa acctgtgccg gtaacaccgg agttgccgtt
    14521 gcttatcgat tctcaactcg taatttgcaa ctcccggatc gcaattttca tctcgcaatt
    14581 cgcaattcgc aactcgcaac tcctgttgta ttgcacaaca cctccggccc tcctgaaaca
    14641 attcaattat ttgtctaaat tgtgtcggta aaaaaaaata atgatgatca ggcgaaccat
    14701 tttatcagct gtgatgataa tgattgcagc gcttgtggcc ggacagaaga ctgacatgtc
    14761 agtgtttaac ggcatcaaac ccaggaacat cggtccgggc ggcatgagcg gcagggttac
    14821 tgcctttgcc gttgacccgc ggaatgagaa tgtgttttac gtgggcacgg cttccggagg
    14881 gctctggaaa acagtaaatg cgggcaccac cttcacccct atttttgata acgaggaagt
    14941 ggcctccata ggtgcgctgg caattgatcc gcagcgccct gacattatct gggcaggcac
    15001 cggtgagggc aatccgcgca acagcgtcac cggcggttac ggaatataca ggagcctgga
    15061 cggcggccgt acctggaagt gcatgggtct tgaactgacc cggtacattc accgtatcat
    15121 cattgaccct gtgaatcctg atgtaatcta tgccggtgcc atcggcaatc cgtgggcgcc
    15181 ccacaccgag agaggctttt accgttccac tgacggaggg ggcacctgga acaggctgct
    15241 cttcaccaat gagacctccg gagtggctga catggtcatg gatccttcca accccggaaa
    15301 gatctttgtg gccatgtggg atcaccagcg ctggccatgg ttcttccgtt cgtcgtcagc
    15361 gggatcaggc ctgtggctta cccgcaacgg aggtaatacc ttcgagaggg tcagtggcgg
    15421 acttccggag gagaccgggc gcatcgggct ggccatagca agatcaaatc ctgattatgt
    15481 ttatgcctat gttgagtcaa agccgtcagc catataccgt tccaccgacg gcggggtgaa
    15541 ctgggaaagg cgaggtgaga ggaacatcgg gggcaggccg ttttactatg ccgagatata
    15601 tgttgacccg gagaatgaga acagggtcta taccctcttc tcaggcgtca atgtgagcga
    15661 agacggggct ctgacatttg accggcgtac agccgaatca gtccatcttg atcaccatgc
    15721 ctggtacatt gatcccctga atcctgaccg catgcttgac ggtaatgacg gagggatggg
    15781 catcacctat gacaggggtg tcacctggcg ccatattgag aacctcccgg tggggcagtt
    15841 ctatcacatc aatgttgata atgagatacc ctacaatatt tacggcggcc ttcaggacaa
    15901 cggttcatgg agaggcccgg cgtatgcctg gtatggcggc ccgctcatca atgagatgta
    15961 tgatttcctg atcgggggcg acggttttga cgccatgccg gtgcccggtg acagccggta
    16021 ctgttatgcc cagtcgcagg gcggatctgt gcgaaggatt gatgtaacca ccggatggag
    16081 caagagtatc aggcctgcgg ccgaagcagg cgaaaggctt cgcttcaact ggaactcagc
    16141 cctggcacag gatccattcg ataataacac aatctatttc ggaagccagt ttgtgcacag
    16201 gagcagcgac cggggcgaca cctggacgaa gatatcttca gaccttacca ccaatgaccc
    16261 ggaaaagctg aaacaggatc agagcggcgg aattaccatt gacgccaccg gggctgaaaa
    16321 ccactgcacc attctgacaa tctctcccag cagccttaag cagggactta tctgggcagg
    16381 caccgatgac ggcatgatcc aggtgactga agacggtggc cggaactgga agaacacctc
    16441 tcagaacatc agggggatgc cgcgcaacgg ctgggtggca caggtgactg cctcgttgca
    16501 tgatcccggc gaagcctttg cggtggtgaa tgactaccgc cagggagaca atgcagcata
    16561 cctgttcctg actcgggact atggaaggaa ctggcagcgg atcatcgacg ataccgatgt
    16621 atggggttat gtgctctgct tcgtgcagga tcccgcagaa ccaaaactga tgtttgccgg
    16681 gaccgagtac gggctttatg tctcctttga ccagggtgac acatggaaca ggtggacaag
    16741 cggataccct acggtttcaa cctatgatat ggtaatccat ccgcgggaac atgacctggt
    16801 gatcgggact ttcggccgtg ccgtgtggat cctggatgat atcaggcccc tgcgggcggt
    16861 ggcttcatca ggcaggaaac tgctctcagg cccgttgact gtctttgatg cgcctgatgc
    16921 atatatggca tcgacaaaaa acctccccgg ctactatttt tacggtgatg caatgtaccg
    16981 tggcgagaac agggaacagg gagcaatgat cacattctat accggttcag ataccggcag
    17041 ggtttctgtc aggctggcgg atgaggccgg aaacgtggta aggaccatgc aggtcaatgc
    17101 aaaaaaggga ttcaacaggt tcatatggca ttttgaccgt gatccgctcc cgcagcttga
    17161 atacccctca ggccaggctc agcagcagga ttccagggca agattcttca ggggttctgg
    17221 cgggactgtc atccccggca tctatactgt cagcctttca tccggcgacg ccagttctga
    17281 ggcaaaggtg agggtgaacc atgacccgag aatggatgcc cctgacactg atgcactgaa
    17341 aaggaattat gagagggcca gggttatcgg ttcacggatc gaatcactca atgcacggct
    17401 cagaccgctg gcacaggtgc gcgagggcct ggcgaagagc gagagtctgg ttaaagaaaa
    17461 ccccggcttc gcaactgctg ttgccgaaga gtacaaagct gtcaaagagg aacttgcaag
    17521 gatggatgag gcgtttggcc gcaggcagga agggctttca ggaaggatag gaggttacag
    17581 ggcgctgctc agtgcgtcag gttctctgac tgctcaggag gagaaggctg ttgccgatgc
    17641 tgaagctgca attgatgaag ggcttaagct tgcggatgcc ttcactgccg gcagctgggc
    17701 aaggtacgtg gagaagctca ggcaggtaac cctttccggt gatgcggtgg tgctgaaatg
    17761 aaaatgcgcc tcagtgcggc gcattcatta ttacttcttg gttttcctgg tcagttcaac
    17821 cacctccatc tcaaagagca agggtgaata gcccggaatt atcgtgtaat agttgccgta
    17881 atagtcataa gttccgaagc ccattgaccc atatgccatt tttgacggca tgattacttt
    17941 ggctttcgac cccatcttca tcttgagcag ggtcattgac catccctcaa tgatgaatgg
    18001 cgagcccacc ctgaacctga atggcgtgtc tgcctcgagg ttgtcgtcga atttctttcc
    18061 gctgaggaag ttgcctgtgt acctgatccc caccgagtca cccctctgaa tcagttcgcc
    18121 ggtaccggcc acattttcga tataataaat gccgtttgcg tcagggctta cggttatatt
    18181 attggtagca acgtagtcct gtataaggct tctctcttcc tcttcgtatt tttttgcagg
    18241 attgcaggcg acgattataa cggccagtat ggccagtgct gcagcgttga attttttccc
    18301 catggtactg ttagtttcca caattcccct caaatttcgt tccaaactat ttaatatccc
    18361 ttattcttat cctgtagatc agtattgccc ttccgggaat ctttgcgccg tcgccggtaa
    18421 gtccgaatgc caggtatggt ggcacaatga acaggtaatc actcccttcc tgcatcatct
    18481 ggagtccttc cgtgactccg ctgccggcat cggtataccc cacccgtatt gtctgtgtgc
    18541 cgctgtagca gggattgccg ttcagcagag tgcattcgta atcgaagttt acattatctc
    18601 ccgttttgac tgccgggccg gtaccatggc ccaggatgct gtaccataaa ccggtgcttg
    18661 tttcggaaaa gctgcggtcg cttttgctta tgtaatcgtt tatggtgttt cggtcctggg
    18721 tgatgagcga cctgttgagt tctatgagtt cggcttcggt gcgcggtgag gccttgctcc
    18781 tgctgtcagg tttgttctgg cagcctgcgg cgatgactgc aatcaccgct atgtatgctg
    18841 ccagtctcat ttcgccatct cccttgcagc ttcctcgagt tccttacgga acgaaggaag
    18901 agtgttttcg aaatgattca ccacctcatg gatggttccc ttcatctccc ctcctgcggc
    18961 attccggtgt cctccgccgt taaagcattt gcgagacagt tcgttcgcgt tgaagctgcc
    19021 cttcgagcga agcgacatct tcacgaaacc ggtacgctcc acgaagagaa cggaaagaac
    19081 aattccgttc attgtcagga gaacatttgc aaatccttcg gtgtcaccct tgacgtggcg
    19141 gaatctgtca agatcttcct tggtaagcca gatatatgca gtatgcagat ccgggtggac
    19201 caccatccgg ctgtgcagcg cgaatccctt gagccttatc ctgtcaggcg agaagttgct
    19261 gaatatatga ttatagatct tttctttatc aacacccata tcaagcaggt gaccaacagt
    19321 gcgcatggtg tcaccggtat agtatccgtg ctcgaaattg cccgtgtcag tgatgatgcc
    19381 gacataaacc gcagtaatga agtcactgtc attgaactca ctgccttcca ttccgttcac
    19441 caggaagtaa accagttcgg aggtggagct cctgctcaca tcggagacgg taaggtcagc
    19501 gatgtcagtg gggtcagggt gatggtctat catgaccttc tttgcgcctg agctgataac
    19561 cagttgttct gcctttccca tcctggaggt ggtattgaaa tcaaccatga tgataagatc
    19621 ggcattgttt atcagtttag taccctcggc agggttatga tggaaatcga cgactttgtc
    19681 aacgtcattc atccaaagga gaaacttctg cagggcgttg ggcgagatca ttgcggcgtc
    19741 cttgccctgt gatatgatcc accttctcag agccagcatg gccccgatgg catcgccgtc
    19801 gggattgatg tggcagatga gcagaatatg accggcgtca tcgatcagct tctttaattc
    19861 ttttgtatat ttaccaaact ctggcactgc gttagccttt taaggcgaca aaggtaagaa
    19921 aaataacatg ttttaaaaat caggtcaaat atccccattc tcatagagtt atgagcaacg
    19981 agatcacatt ttcaatcatc aagcccgatg cgcttggaag cggcaacgcg ggaagcatca
    20041 tttcacgtat taacgaggca ggattccgga ttgttgcgct taagatgctg cacatgacca
    20101 gggaacaggc agaaagattc tacgaggttc accgcgagag gcccttttac cccgggctgg
    20161 ttgagttcat gtcatcaggt ccggccctgg tgatggtgct caggcatgcg gatgccgttg
    20221 aagagttccg caggctgatc ggggccactg accccgccag ggcactcccg ggaaccatcc
    20281 gcgcagattt cggcacagat gtcaggatga acgcggttca tggctctgac tccccggaga
    20341 atgcactcag ggaagcctcc ttcttctttt ctgccatcga gatattctga gaaaacaata
    20401 ttcctgtttg cttgttcagg taaaaaattg aaatgactgc tactgtatcc cctgactttt
    20461 cggacaaaaa gaccctcgaa aagtattcat cagcctatac cctctccgat atggagattt
    20521 ttattttccc cgaattgttt taccccctgg ttctggcaaa tataatgtca cctgaaatat
    20581 ggcggtggcg tgatgacccg tggttcaggg acatgcaccg gaagaacttc atcagcaggg
    20641 ccaacaggat caaacagtac atcattgaca attacatatt caatcttgat ctcgagacct
    20701 ggggcctgac agacaaggag agagagctgg accggttcag tgatttcttt gacacggaac
    20761 tgctgaagca gtcgaatgca cttttcggtt acgaaggaga caaatattat ttcagtattg
    20821 acatccgtcg ccatttcggg ctcgataagt atgaatcatc tgccataccc tactggaaga
    20881 ctgaaacagt ggaggcaatg acagcattca ggcaccggga acattacact acaggcgccg
    20941 gcgagtgcgt cagcctggcg gcactctatg ccgcagccat gttcattgtt ggcgaggttc
    21001 ccctggaaaa gatcttcatg atggccactc cgcttcactc gcagaatttc attgatgaga
    21061 aggaagggct gctgacaaac aacaggagga tcatgaccaa aaacatgtgg ttcaacggca
    21121 cctcactgtc aggaaaagcc agaagagccc tcgaaaatga gaaggtgact attgtttcgc
    21181 acatcaccgg ccacatccat actgtttatg aaagggcaac catcgacagg gatgcatata
    21241 acaccttcac caaaaagctg aggagcttcg tgaagtcgcc ccttacccct gccatcttca
    21301 tcaacttcct caggttcaaa tcggagtaca aatgtctctt ccagtatcat tacctccgga
    21361 cgggtaccag ccattacatc accctcgata agctttttga atatgagcat tcgctgaaag
    21421 ccagcatgaa cgaggagacc cgtgacaagc tgcttgccga ggtagacagt gaggaattcc
    21481 agtatgaccc tgttccaggc aagatcatgc tgaatgaagt tgaggcattc attaaacagc
    21541 gcagggactc tgacctgcag gtcattgaag ttgagttcac agacagcttc cgcaccgagc
    21601 acactgattg cgtaaggaga atgtttgctg acatcaggga gttcataata accgatccga
    21661 ggctcccctc ggctgacagg gaatttgtgc cggagatcca tccgcagata tcagtaaatg
    21721 acagtcgtga cgagataatc agcaagatca ggggtcttgc cggagtatcc gagatggcgt
    21781 tgctgacgct ttatgcttac cgggatatgg agatggctga ctggaggccg tttgtaaagg
    21841 ctgccatcga gaggaaccct gtatgtcatt cagatctggg agggcgtaac gccgacgaga
    21901 tatatgcact cattaataaa ctgaccaatc aatcaattta tgactcgggc aggctggccc
    21961 aacctgacga ggtctggaat ttcgggaggg gtgacggcgc tgaaaaagct ttcctgatgg
    22021 ctgatgccct ggtcttcaat gaccccggcg ccgtggtgaa aatcttactg gaagatgacc
    22081 aggcagtggt tgaatacaat tacaggtatt tccggtttgc aacagtcaag ggactcagga
    22141 aaaagataac aataagcagg aaggaataca cagtatggtg agcaggataa ccctgatgat
    22201 cacgcacgga agaaacaggg cctgtcagac ggactcatgc tgcccggctc tttcaatgta
    22261 acattatttc cctgaccact tcctcccctg ccctgctgtt tatctggctc cttagcgcat
    22321 cgcgcagcat catcagttca ttccttacga ccgatgatgt caggtagatg tgaagcacgc
    22381 catccttcag gtatgtcctt ttggtccggg ccgtaatgac tttcccggcg atgttttccc
    22441 atatattcag cacagcggct tccttcagcc ctttttcaag ccggtactcc tttatcaggt
    22501 ctccgagagc ctctcccagg gtgatggtct tgttcctgcg catgtcttta tttcactccg
    22561 ttggtgattt cctcctcgat accctttttc ccgatacggt aaagccggaa gtcaaccccg
    22621 gtattgtcaa ggatgcggtg aatacgatcc tgctgggtgt cagtaataaa aacctgcccg
    22681 aattcacctg acccggcaag cctgatgatc tcctcaaccc tggtctggtc gaacttgtcg
    22741 aatatatcgt caagcagcag agcaggcgcg aatcctatca tcctggttat aaggccgaat
    22801 ttcgccagct tcagggccac gatgaatgac ttctgctgac cctgactggc gtttgtcctg
    22861 gcggaatggc catcaatctc aaagaccagg tcatcacggt gaattcccat ggtagtaaac
    22921 tgcattgcat agtctctcac ccgtgagtcg gcaagctgtt gccggaaatc accgctgccg
    22981 aggtgtgagc ggtaccggat tgacactttt tcagcgccgc cggagatttt ttcgtagtaa
    23041 gagcgaaaga gaggcagcag gttttctgtg aggttgcgtc gtgcactgtg gatcagttcg
    23101 gcctcagggg ccatctgttc gtcatagatc tcaagcaggc ctgaattgtc cgggccgctg
    23161 cccctgagta tcctgttgcg ctgcatcagg gccttgttgt agcgcatgtg tgcctcgagg
    23221 taggacgggt catactggct tatgatcagg ttgaggaagc ggcggcgctc ctcgctgcca
    23281 ccggtgatga gggtgctgtc agccggcgag agcatcacca cagggtattt tcccacatga
    23341 tcagacatcc tctggtactc cctgccattg agtttaaaca ccttctgctt cgttttctgg
    23401 aacgcacaga agagctcatc actgttttcg tcggtgacga acgttcccct tatcatgaag
    23461 taatcgtctc catgcctgat actgagggtg tcagagctgt tgaagaaact ctttgtaagt
    23521 gacaggtaat gaatggcatc gagaatattc gtcttcccga caccgttgtt tccgacaaag
    23581 cagttgaaac cctgagaaaa ttcgagggaa accccttcat agttcttgaa gttggtgagt
    23641 tcgagcctgg caagatgcat catcttagtc tgctgttgcg gaggctccaa agttacgaaa
    23701 agaaacggcc ggggcaagtc cggaaggtgt aatgccggag caccgcaggg caccgagcat
    23761 ttttttaccc atttgttttt taataaagcc agaaatacta cttttgcgaa gttatttttt
    23821 gacctgtaac agagaacatt gatatgagca agaaaaagga gaccacaaaa gggatcgaga
    23881 acgttgaaca gacgctgaca aaaaccgaac agttccttga acagaattac aagcctcttc
    23941 tgtacgtgct tgccggcgca gtggtgctgg tggggatagt gtggcttctc aggatgtata
    24001 caagcaagaa gaacgatgag gctctgagcc agatgttcat ggcagagcag tatttcggac
    24061 aggactcact cagcctggct ctcaacggtg acggaaacaa tcttggcttt atcgatatcg
    24121 caaaggaata caaagccacc aggtcaggca agcttgccaa cttttatgca ggagcatgtc
    24181 ttatgcatct cggtcagtat gaagatgcca tcagctacct gaacaagtac aaacaggatg
    24241 atgaggttat agcgccacag gcgaagggtc tgatcggcga tgcgcgtgtt gagcttggag
    24301 acatggccgc aggaataaag aattacctcg aggccgccga catggctgaa aacgcgttcc
    24361 tcacacccat ctacctcatg aaggcaggaa tgctctacga gagcgacggc aaatgggccg
    24421 atgcactcaa actctacgaa cgcatacagg ataagtaccc ggagagcaat gaaggccgga
    24481 gcatcgacaa atatattgcc agggtcaaac tgcacatcga ctgacaggtg gcaacccgcg
    24541 atctatcgga ttatgatccc tcacgtgtgc ccgacgcctc gggcatgagg ttcggtgttg
    24601 tggttgccga ctggaacagc gaggtaaccc atccccttct tgacggtgcc gtaaagacac
    24661 tgaagcgtca tggagtaact gccgacaata tcatcgtcag gcatgtcccg ggcaccttcg
    24721 agctgactct gggagctcag tggatggctg agtatgagga tcttgacggc gttatttgcc
    24781 tgggatgcgt cattcagggc gagacccctc attttaccta tatctgccag ggagtgaccc
    24841 aggggatcac tcagctcaat ctcgattaca atataccatt catattcggg gtgcttacca
    24901 ccctcgacct ccggcaggca cttgacaggg ctggcggcaa gcacggcaac aaaggcgatg
    24961 aagccgccgt gactgctata aagatggccg cacttcaggg gctgatggag aaataatcat
    25021 ctgcctgaca gtcatcattg tatccggatc cccggattat ggcgtttgta tgttaccggc
    25081 cgcccaggct ggtctatctc ctgcaaagcg tcctgtaagg gcatgaaatg caacggcgcc
    25141 ggaaagtggt catccggtaa tcctcatctt cagcaaatat cctgttcagc gtcatgtcca
    25201 gcccgcttcc gaacgaggtc atgaattcat tccattcatt catactgctc tccgggccat
    25261 ccagtccggg tacaggtgca tagggagaaa agtcatccga ggtcagctgc tgtatccagt
    25321 aaatggcagg caataccatt ctgccggggt gtttgttttc aatgagcgta cagtacagca
    25381 gagcctgaag cagggcatca ttgcgcttct ccttctcctc atcgaacagg ccttcagccg
    25441 tcaccgcctc ccttttcggc gccccggtct tgtaatcaac caccctgatg gcacccctgg
    25501 tcagatccac cctgtctacc cttccgccga gaacaatcct tttctctccc tctctgcaag
    25561 taacggctcg gactcctgaa aaatcatctt caagattgag caaaaccagg tcggagttgg
    25621 ctccgtcata ttgaagcagc tcacgtgcat atcgttcaag gacgtcaatg atgatcaccc
    25681 ccctgcctga cagaagtgtt tcacggtccc atctcatctc atccatagct gcggcgatga
    25741 tggtttccct tatgttttca cgtgatctgg ccataagggc tataatggaa cctgcattct
    25801 ccttccccct gaggggctga tacattctcc tgagggcatc atgcatgatg ttgccgaacc
    25861 tgcgctgatc tatctccttc tcaagtacct cttcctcggg cataccgcac acatacctgt
    25921 aatagaaccg catgcggcag ttcagccatg tatttacagc gctgggagag agatatttgt
    25981 tatccctgtt accctcgccg tagatatcga ggagagtccg gttatgagcg gctgttttgg
    26041 caagtgtctc cgctatcatg ccgctacggc cgacactgat atggaccgac cgatgccgcg
    26101 gtgcgaagag cgggttgtaa ctcatcctga ccaggtaccg gctcatctcg ccgctgttca
    26161 gtccctgggc ggtggagtta tagagaaacc aacccttttc gggtttgcgc agcagcctga
    26221 aaaagtagta ggagtagatc gattcgtgct cgttaaccgt gggaaggccg aaggcccgcc
    26281 tgatattgta cgggatatag gtgttgtcat atgacatccc ggggaagata ccctcattaa
    26341 gtgagaggat gattatattc ctgaactcga gggcacgtgt ctccagcaca cccatgactt
    26401 gtatcccccg caggggttca ccggagaagg gcactatcat cttgcggaac aaccggtcaa
    26461 ggagcctgag gcaagtctcc ggcatgagat ccaggtcata gctccttatc aggtttgaca
    26521 gacgcacggt gctgcccatg gccatcctca ggtattcacg gtcagtgctc agcttcattc
    26581 cctgctcctc tgcagcatag gtggcttcct cgagcctggc catcaccgat gccaggtggt
    26641 cgggtattcc ggctccccct tcgggtaccc tgaagaggta atcgaggcgg aaccgttcgg
    26701 cgagcatggc cgcagtcacc ctgatcatgt tgcccttgat gatctccttt accaccgtat
    26761 cgccttcatt gcctccgaga agcctgatgt actggtgacg cagcagtgcc atcacatcat
    26821 cgctccggaa ggagagaata ccgttgctga cccgtgctga acggatcagt gccatcagtt
    26881 gcctgaggaa ggagtaaagc gttgtgaacc ggaagggatg gcccatggtg acattcacat
    26941 cttccacaga tgcaggcaac gaaccaagaa cgggcatcag gagcttttcg tcagcaagta
    27001 tgactgctgt tgatgtcaag tcgtctcctc ctgttattcc ttcattttca agcaactgcg
    27061 ccatcatcgc agcctgggcc gtatcggaag gggtgtcaat gatatgccat tcgccgcggg
    27121 gtgtctgatg tctccctgaa gatgcaccgg agccaatttc tgttcccggg cccgtacctg
    27181 aaccggaacc tgtgcctgag cctgctcctt cccctgaaag ggagtttccg aagatgctcc
    27241 tgttctccct gatgaagagg gaggctttgt gttccggatc atccatgaaa aagtggtcgt
    27301 catcccagta aaattcagcc acccccaggc gtttcaagtg catgaacaag gttttctcac
    27361 agttattcag cgcattgagg cctgctatat gccatcttct gccctccggc acctcaagca
    27421 acccggactg tgccctcccg gccacctcac ggcagagcat gccgtcaccg gccaatccgc
    27481 gtgctctcat cgcttcccgg tagccctcat aaagcggacc gagcctctgc cacacggaac
    27541 ggaaccgcga gcgggcttca ctgcctgctg aagagggatt gaacgacttc cagaagcctc
    27601 gtattatctc aacctgttca tcagtgaggc ctccgaactt cacatctatc tccttcaggt
    27661 ctgagatatt gctgtagagt ttgccggcat cggccatgta caggtcaata tcattgaagt
    27721 cattgattat cacctctccc catggccaga aatcgtcaaa gctgatctcc tcaccaaaca
    27781 ggtttctgta gatccggtag agttcgaata tttgtgtctc ggtatctgcc gggatgaggt
    27841 cagtgaacga acggaacagg tcgctgacgg tgagcatcga gggcgcccat gaaggtttgt
    27901 cattcatcct ggcgagggcg cgtgcgagcc acagtccggc tctgcggctc ggcagcacaa
    27961 ccgtctgttt ctccatctcg tttccgtgac gctcacggag cctgtatgcc actttttcaa
    28021 ggaaatccat catcagatca ttttacagag cctcccctgc catgtattgc gttcttcgag
    28081 gcggtgttca aagagcctga acagttcgct gctcctgagg tgccagtcgc gcgggtcatc
    28141 aaggagccgc gggggcgcct caccgcttat gcggtcaatc attatgccgg gcgagagccg
    28201 ctcggtaaat gtgatcacca ggtcaaggta ctcctcaagg tcatagaggt caaacgcgcc
    28261 gggattcttc ctgtattcca tggccatcgc tgttcccttt atcagctgca actgtctgat
    28321 cttgagcgat gtcagtggca gtgatgagat aacctcagcg ctgtcagcga tggcgctgcg
    28381 gctctcgccc ggcagcccga aaatgagatg cgcccccgcg ttaagtcccc tcagctcagt
    28441 ttccttcaag gctttcatag ccgtggtaaa atcatgcccc cggttgattc tccggaggat
    28501 atcatcatta accgattcaa tgccatattc aatcatgacg aagtagtcgc gggccatcac
    28561 tgccagcctg tcgagcagtt caccgttgat gcagtcaggc cgtgttccga tcaccaaccc
    28621 tgccacggag gggtgattca gagcttcatc ataaagagca gtaagtttgt caatgtctgc
    28681 ataagtgttg gtatatgcct ggaaataagc aatatatctc tctgcgcggc gatatctgcg
    28741 gcgatggaag gcaattccct gctcaatctg atcagtcaca ctcttttccg gcgtgcagta
    28801 gccgggaatg aaggcctcgt tgttgcagta tgtgcagcct ccgtacccca gtgtgccgtc
    28861 acggttcggg caggtgaagc cggcgttgat gctcagtttc tggactctcc ctccgaacag
    28921 tccggcgaac cagtcggcat atgagttata tcttctggcg gttccccaca cgtaattctg
    28981 ctccccgttc ataccttctc aatgatatcg atttccctgt acctgtatga cacttctccg
    29041 ttcatgcctc ctcaatcaga tccttttcga cataccatag ccacgatttc acttccggga
    29101 agcccatctg cctcaggagc tcgcggtagt ctgcggcctg ctgtttatgg cgtggcgagg
    29161 gctcaccgaa tttatagtca accactgtca ccctgtcacc gctgatcatc accctgtcag
    29221 gccggcgggc ggcgcccgtg gggaggatga tggtagcctc ggtcatgacc gctgtggtgc
    29281 cgtcaaacca tgccttcacc cgttctgccg agagcattgc gtggaggcgg gccacagccg
    29341 tgtcacggcc tgtcagcggc atgagcccgg tggcgcaggc atgacttacg gccttttcaa
    29401 tatcagccgt ggtggtgacg cggctgagga tctcatgcat cattatgcca taggccctcc
    29461 cgcccggttc ggcgagtttg acctcatcgg cagggagggc gccgcccgtc ctgagcctga
    29521 gggtgccgcg gggttcactg acgacatagc ctgagatctc cagccagggt tcacgctctt
    29581 tggtctcaac cgccggtagc tctccgcatt caatatgcct ctttgtttct tcatcagccg
    29641 ttttgaaatc accgggcagt tttgccagcg cctcgttcag aaggctggcg gtattggcgc
    29701 cggagccgcc tgccggtccc ctggtcacct cctgggtcat caccaggagt gcatccacgg
    29761 ccctggtgaa agccacataa agcaggttca cgccgtcgag ccagtcggag gccctttcca
    29821 tcttcgcttc cccggagaag agtgactcgt cgaggcggcc tgacatttcc ggaagcacga
    29881 cgggcatagg tgcaaaggga gcgggcacat tggtgaccca cagcagagat ctggcgtacc
    29941 ctggctttga gaactcccat gaagcaaagg gaacgatgac taccctgctc tggagtccct
    30001 ttgccttgtg aacagtcatc accctcatcg cttcctgcct ttcagactgg ctcagggtac
    30061 ttttgcatcc ctcatcgtcc caccactgca caaacgctga gatatcagag ctgtgccggc
    30121 ctgaccaggc gagcaccaca tcctggaaag agcttatata ggctgtattt gccctgttcc
    30181 ggccaaggcc gaagaaccgt atcatgccct ccgtggcgct gtaaagtgat gcattgcgga
    30241 atgattctat agcctcttcc catccttcag ggagcgactc tcctccgcca tccggggtgt
    30301 caccgctgta cacaagccgt tcatctccgc cggtggccag aatatatgaa cggactatct
    30361 ggctgcggtt gatgcggctg gccggatcgg tcagatacct gagggatgct gtcatcaggg
    30421 tcacagccgg attgcgctca aggaggagcg actcgccgga agtgatctca taattatacc
    30481 gtgcccgctg tccgggggta cataatgaac tgtactcaag gatgcggctg atgatgctct
    30541 tgccctcctc gttcgtccgg cacaggaaca ggatgtcgtc agccctgtac ccgtgttcct
    30601 gcagccgttc gatcactcca ggaagatcac ggagcacccg gtctttccac ccttcgtcgt
    30661 catttttcct gtaaagggtg acactaacgt aaccgcccgg tttgccggga gtgccctgct
    30721 gctcagagtc cgagtagaca tcactgatct tcagcccctc gtatgccagc ctttcatcac
    30781 atgaggccgg aatggaggca gggctgaaaa gggcgttgtt aaacctgatg acatttgcac
    30841 ggctgcggta gttggtgctg agatgaactg tttcaacggc atcaccgaaa acctgtgccg
    30901 cctcactgtg aatgatgcgc cagtcgctgt tgcgccaccg gtagatcgac tgttttacat
    30961 cgcccacaac caggttgtcc tgtccgcgtg agagagtctc ggcaatcagt ggcctgaaat
    31021 tgttccactg aatgcgggag gtatcctgga actcatcaat gatgtaatgg tcatatgcgg
    31081 cgcctatctt ctcatagatg aacggggtgt catcatcggc tataagtttg ctgatcagtt
    31141 cacccgagtc agaaagaagg aaaatgttct gttcgtgggc ctgttcacgc actctctcgc
    31201 tgattgcacc caggatacct attacatgta tggttctgac ctgtgccagt gcagatacat
    31261 acacgccata gcgcctgtca aacagatcta atattttttt caccacatcg tcaaggcctc
    31321 tccgtgaagc atcgccgaag gcgctgacca cagcgggtct ggcagtgccc ggaaagaatt
    31381 taccctccgc tgctgcctta acccaggtgc tgttgggccg gcgggtgtca ccctgcgcaa
    31441 accgcctcag gctttcgccc actcctcccc tgcttttgga aagaaaatcg tcagtggaca
    31501 aaccgcattc ggtatagatc tttaccccct ctgctgcaag gttcttaagg tcagtttcaa
    31561 atcctctcct gacagcaaag actcttgatg cataatcagc catcacaccg tagtctccta
    31621 tcattttacg gtcatcggtg ctgagctgcc tgaagtcttc cctgaaaacc tgcgcggcca
    31681 cttccatgat ctcccttctg atatcccatc ccttgctgtc atcaaggcgt gaagccacat
    31741 atgacgagat ccagtcaagc agtttgccgt cagtggccac ctcagccagg aggctgtcca
    31801 cggcatcaga gagtatcgcc tggtgctcca gttcgatctc atagcctgcc ggaatgtcaa
    31861 tttcccttgc aaaagccctg atcacccttt ggaagaacga atcgatggtc ccgacggcaa
    31921 agcgggaata gtcctgcagg atgttgtcaa gcgcaaccgg ggcatttctg cggattgtaa
    31981 ccacagcagc ctccctgtcg ggccacattt caggaaagaa tttgcagagc cacacagcaa
    32041 attcctttct ttccctgtcg cccttttcat tctccagtcc cagggtattc agtcttctga
    32101 gtatcctttc cttcatctca gccgcggcgg agttggtgaa ggtgacggca agtatccggc
    32161 ggtaagcccc cgggtcatga agcacctgcg agaggtagaa ggcagtgagg gcgtgggtct
    32221 tgcccgaacc cgccgatgca ctgtatttca ataacttacc tggtaacttc atcttctgaa
    32281 aaatagtaaa aaaagagtca tgaaacggag gggagagaag gtagttatta acatattgag
    32341 ttaaagagct acctttgccg cgtgaaaaag aaggcaatag taggattgtc agggggggtc
    32401 gactccagcg tggctgcatg gctgctgaaa gagcagggct atgaggttga aggaatcttc
    32461 atgatcaact ggcatgacag tcgcgggttg ctgaagggcg attgtccgtt cgacgaggat
    32521 atcacctttg ctgagatggt tgcaaagaag ctcggcatca ggctgcgtac cgttgacctt
    32581 agtgtggaat accgtgagag ggttgttgat tacatgttca gggagtatgc cgccggccgt
    32641 actcccaatc ctgatgttct ctgcaaccgg gagatcaagt ttgactcctt cgcgcgtgaa
    32701 gcgctgaaac agggggctga ttatattgcc acaggtcatt attgcaggaa tgagcaggcc
    32761 tccacggccg gcggcatggt caacaggctg ctggcaggtg ttgacagcaa caaggaccag
    32821 agttacttcc tgtgccagct gagccagggg cagcttgaca acgtgctgtt cccggtgggt
    32881 tcacttacca agccggaggt acgcaggata gcgacggacc tcgggctggc cactgccgtc
    32941 aggaaggatt cacaggggat ctgttttgta gggaaggttg acctgccggt cttcctgcag
    33001 caacagcttg aagcaaggaa gggaaggata atttacgtac ctgacagcga agctttcaga
    33061 agctggcctg acggactgcc ccaagagggt gtcaccaccg cggagttgtc agaaatgacg
    33121 ataccgcact cactgagtcc gggcgacggg gtgactgcag gggtgcacaa cggcgctcac
    33181 tttttcaccg tgggacagag aaaggggctc aacctcggag gatacaggga acctctcttt
    33241 gtcattggca cagacacggt aaacaatata atctatgtcg gagagggcca actgcaccgg
    33301 ggactttaca gaaaagggct cttcataagg aagactgagg tgcactggat aagggaagac
    33361 ctggcaatga atcctggcga agaccgtgat taccagttac gtatccgcta tcgccaaccg
    33421 ttgcagcacg cacgcatcca tgcccgtgat gagggttatt acataatctt tgaaaagtcc
    33481 cagaggggaa taacaccggg acaatttgct gcctggtatg acggtgaaga attgcttggt
    33541 tcaggtccga ttgatgaaca aagtggaata attggctgtt aagcatttga tttgtccaca
    33601 atttttggta tatatttgca gttgtatttc aaaacaggac caaaaaacta aaaaaacacg
    33661 aaaaaactaa tcctttccct tgtctttgtt ctactggcgt ttggtctgaa cgctcagagc
    33721 aatgttatca aaaccaatcc tctcggcctg gctttcggaa gcttcaacgc cacttatgag
    33781 agggtgtttg gcacaacctc ttccctgctg gtaagaggtc agtacatgta caagctgctt
    33841 ggagtagatg tcagccttgc aggtgcaggt ctgggataca ggtactactt cacacatgca
    33901 aagaaaccag tacccgccgg tttttacatc aatcctcagc ttgcattttc atttggcaaa
    33961 gtaaacgatg acacggaagg caattattct gccagcacga taggtttcgg tgccgagctg
    34021 gggtatcagt gggtatggga aagcggtttt gtccttgacc ttggtatcgg gccgatgtat
    34081 acgattgcca aaggagggga cagtgagagc agtattgatg gcattatgcc aagcgccact
    34141 ctcgcaatcg gatacgcatt ctgacaaaaa tcacagtgga caaattataa aggcaggcgg
    34201 agtgatccgc ctgttttttt gtatcaggca ataggcaatt ggcaatatgc agtagtgaaa
    34261 ttactaacaa tcaggtcgat cataaacata tcattgtcaa cactactgcc tacagccagc
    34321 tgcctgccta agacagcctg tcacggtaat cagcatatcc gaactccctc acgagcctga
    34381 attgctggtc agctgtccag atgccgatgt taggatgcgg tacgccgttg aaggtagtgg
    34441 ttttcaccat tgtgtagtgg atcatatccc agaatatcag cctgtcaccg ggatgcagct
    34501 cacggtggaa tgaccagtcg cccatgaagt ctcctgacag gcatgagttg ccgcccatgc
    34561 ggtaggttgg ttttcccgga acagggtcaa atgccccgat aatgacaggt ttatagggca
    34621 tctcgaggca gtcaggcatg tgtgcggtga atgaaacgtc catgattgcg gtctttatgc
    34681 cgtcacgggt aatgatatct tccacggtgg ccaccagttc gcctgtttcc caggcgaaag
    34741 cgctgcctgg ttcaagtata aggtgaatgc ggtgtttctc tctgaatttt ttaaggaggc
    34801 caatgagatg gccggtattg taatcacgac gtgtcatcag gtgcccgcct cccatgttga
    34861 gccacttcag ctttggcagc aggtgtccga atttttcctc aacgactgcc aggacctttt
    34921 cgagcgtata tgaatcagat tcaaacagga catgaaaatg gagtccctcc accccttcag
    34981 ggagtccgtt cgccagctcg gctgcagtta caccgaggcg ggatccgggt gagcagggat
    35041 tgtatatacc ctgggaaatt tcggagtact ccgggttaat ccgcagtccg ggcgagatat
    35101 gctttccctc gcgatgaagc gccggggcga acttcctgta ctgttccagg gagttgaatg
    35161 ttatgtgaga gctgtagcgg agtatcgtac cgaactcatc cctgcggaag gcaggtgcat
    35221 atgtatgagc cggagtaccc atctcctcga aacagagcct ggcttcatgt atggagctcg
    35281 ccgccgcccc ggagacatat tcgcgcacca cagggaatac tgaccacatg gcgaagccct
    35341 tgaatgccag tattatttcg gcaccggaag catcggccac atgcttaatc aggctgaggt
    35401 ttttcctgag tctctcctcc tcaatca
//
LOCUS       DAOPXU010000020        35063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_1134_length_35063_cov_11.296646, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000020 DAOPXU010000000
VERSION     DAOPXU010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 35063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35063
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1134_length_35063_cov_11.296646"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(303..1943)
                     /gene="hcp"
                     /locus_tag="PKK03_03390"
                     /gene_synonym="priS"
     CDS             complement(303..1943)
                     /gene="hcp"
                     /locus_tag="PKK03_03390"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNX83469.1"
                     /translation="MFCYQCQETAKGTGCVVKGVCGKTDDVAQMQDMLVWIAKGISIL
                     GTIARQNHYSNAKVDRFVQEALFTTITNANFDRVKIIEKIHEGIALRDNFAGEMKAHG
                     ITIPGNLNECVAWKPVSEADLAAKAAEVGVLSTPDEDVRSLRELTTYGVKGMAAYAEH
                     AGNLGFNSDSVDAFIQKALAATTDDTLSADELVALVLETGKHGVEVMALLDKANTTTY
                     GNPVISEVNIGVRNNPAILISGHDMKDMEELLRQTEGTGVDVYTHSEMLPANYYPAFK
                     KYSHFVGNYGNSWWKQTTEFETFNGPVLFTTNCIVPLRDSYRDRVYTTGSSGYPGCPH
                     IPDREPGKEKDFSAIIEHAKRCPAPTGIEEGTIIGGFAHNQVIQLADKVVDAVKSGAI
                     RKFFVMGGCDGRMKSRDYYTEFAQKLPKDTVILTAGCAKYRYNKLKLGDINGIPRVLD
                     AGQCNDSYSLAVIALTLKDVFGKNDINELPIAYNIAWYEQKAVIVLLALLYLGVKNIH
                     LGPTLPGFLSPNVVNVLVEKFGIAGITTPDEDMKILLN"
     gene            complement(1994..2917)
                     /locus_tag="PKK03_03395"
     CDS             complement(1994..2917)
                     /locus_tag="PKK03_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX83470.1"
                     /translation="MQRDIIKIDEEKCNGCGVCVPNCHEGALQVIDGKVRLVSELMCD
                     GLGACIGHCPEGAITIEKREAEPYDEIAVISEMVKKGPNTVIAHLKHLKDHHEYGYLE
                     EGTTWLEVHGSELSFDAAEVLDAVSGKQGQAQAAGGAHLQHQHQQHQHGHRHHGGGCP
                     GSAERLIAPAPVFGTASAESPSMLTHWPVQMHLVNPNSPAYRGADLLLAADCVAFSMG
                     SFHSRFLKGKALAIACPKLDHGTETYIDKLTVMIDTAKVNTITVLMMEVPCCGGLLQM
                     VRAALARASRKVPVKTVVVSIEGKILQEEWM"
     gene            3027..3698
                     /locus_tag="PKK03_03400"
     CDS             3027..3698
                     /locus_tag="PKK03_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNX83471.1"
                     /translation="MRFPVLSNSPLFRGLTDSEIEKLIAAVNFRVRFYHAGAVAALAG
                     EEITSLMIVLSGSVRGEMSDLSGRTMKIEDINPPQALAAAVLFGEGARYPVTVIANSD
                     SELLIINKDDYLAMMAGNKRILSNYLSFICGKAQFLSGRLRFHSFHTIKGKFAHYISS
                     LPGASTGRVVIDRPQQELSEYFGVTRPSLARAIGEMEQEGLISVDRREVRFLNLKGLS
                     ALTGA"
     gene            complement(3685..5781)
                     /locus_tag="PKK03_03405"
     CDS             complement(3685..5781)
                     /locus_tag="PKK03_03405"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNX83472.1"
                     /translation="MNAGMRVPGRVSMTFLLLILLSGSVAALSPRVEKGVLDLSKIQQ
                     GDEFTVRMNGEWEFYFGTFINGTPGDICDTLIPDCYATVPGYWSDYTVDGRKLPRFGY
                     ATYRALILLPRGYRDRMGFDMPVFDTSYEITINGVTMARNGTPARTRAESVPSYEPLF
                     FSYVPKSDTLELLIRVSNYEHRRGGFWMPMKAGTFHSIQTNFTNQWFISILVSGILFA
                     SFLFFLIFYVLDRRDRKLLMFAVLVLCLALRPFLSAPYLATIVDIRNWNLIIRGEYLI
                     LLFMVTSGMWLAYLIYPARWFRRFAIAADVVFVPGVAAVVFLPVRIFSWSVFGIQAIA
                     MLAMLYVVSMSIRGIMKGNRRDILYLLAFLAILFGLVSDILMSNALEENQQLYILSFL
                     ILIFVLIQATLLIADWVRRGKEREKLSGQLEELNHSLESRVEERTRELTDRTEELNAR
                     NQQIALQNKKLTETISLKNKIFSVIAHDLRSPVVNILYTLYLLKEEEFRDRTESLADS
                     CIQYSQQLISLLENMLVWGRGQEDMIKYAPAENDLADIILTNISILKDSADRKNIDLN
                     FTQVGRSIGWFDRDLVDIIIRNLLTNAIKYTYHGGKVTVHVKEREESSEWVTIRICDN
                     GVGITPERQKRLFTGHEIESTPGTDSEKGTGIGLRLVYELVALSKGTITVESTPGSGT
                     CFTVILPGSDQAFRLR"
     gene            complement(5785..7878)
                     /locus_tag="PKK03_03410"
     CDS             complement(5785..7878)
                     /locus_tag="PKK03_03410"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNX83473.1"
                     /translation="MKKILIMMFIASLGVISCKKESCENPFYCDWDTPFEVPPFDKIK
                     IEHFKPAFLKGMEEEMAEVNAIISNTDEPTFDNTIRAMQFTGKLLQKVQRAWGPLSGA
                     NTNDSIQALQREMSPLFSRHSDDIALNEKLFERVKSVYENREKFNLTPEESKLLEDEY
                     LGFIRSGIGLSSENKEVLRKLNSELSLLGVRFGENLLAETNAFTLVIDNEQDLSGIPE
                     GIRAQAAAAARAKNMEGKWLFTLQNPSYIPFMTYADNRALREKMFRGYSMRGNNGNEH
                     DNNKILADIIRLRVEKARLLGYESHADYVLERNMAKEPETVLNFLSQIWEAALPVAKA
                     EAAAQQELIDRESGKFKLEPWDWFYYTEKIRKEKYDLSDEETKPYFSARNVTDGMFYV
                     ANRLYGLEFSERNDIPLYHPDVKTWEVKRDNKHVGILMIDYHPRASKRGGAWCSGMRP
                     QRIDENGKFVTPLVSMVTNFTPPSGDTPSLLTPDEASTLFHEFGHALHNLLSNTEYTS
                     GVSRDFVELPSQVMEHWVFEPEVLKVYAKHYQTGEVIPDALIEKIEKAGKFNQGFTTI
                     EYLAASSLDMEYHSLTEPVELNIGEFEKKAMEKYGLISEIIPRYRSTYFNHIIGGYSA
                     GYYSYIWCEQLDSDAFKAFKETGDIFDKEVAARFEKEILSRKGSKDELEMWIAFRGRE
                     PGIEALLENRGFINR"
     gene            complement(7986..9728)
                     /locus_tag="PKK03_03415"
     CDS             complement(7986..9728)
                     /locus_tag="PKK03_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HNX83474.1"
                     /translation="MKQKIRFSLLYRDMWQSSGKYQPRVDQLTAVAPYIVEMGCFDRV
                     ETNGGAFEQVQLLYGENPNTAVREWVKPFNKAGIQTHMLERALNGLRMYPVPADVRKL
                     MYRVKKAQGVDIARSFCGLNDHRNLELSVKYAREAGMISQAALSITHSPVHTVDYYMG
                     IVDHVVEYGADEICLKDMAGVGRPASLGELVRRIKSKYPGIIVQYHGHSGPGMSVASM
                     LEVARAGADYLDVAMEPLSWGMVHPDVIAVHAVMSDAGFDVPEINMDAYMKVRALTQE
                     FINDFLGYFIDPRNRQMTSLLIGSGLPGGMMGSMMADLKGVHQGINAALKARSKPEMT
                     EDELVVQLFREVEHIWPMMGYPPLVTPFSQYVKNVALMNIVSLVNGKERFAMIDPNTW
                     DMLLGRAGTLPGPLAPELVELAGKAGKEFYEGNPQENYPDALDTYRKEMEDNGWSAGP
                     DEEELFELAMHDRQYRDYKSGVAQKRFLKEIADKQAELSQGTPAKTPPRKLLHSPEAG
                     IAFLYSGATATYWPDEEMLNVGEKVKKGEILMYIMIGKTIREVVSDRAGTVKKILVRS
                     GDKISKGDPLIEFS"
     gene            complement(9934..10392)
                     /locus_tag="PKK03_03420"
     CDS             complement(9934..10392)
                     /locus_tag="PKK03_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83475.1"
                     /translation="MKRISLVLVLFAAFSLLSFGKVIIRGESNTPFGAYTIERLDETV
                     MLAGEEMKCYLISYEKSPLQVKVLVDKEKKCKNYLVITDELSVMYTCNGEFFGVSRLG
                     EKYRAAGLATTDEKLDRYDYFHQKVISQGATEEFDATMLIASYFPELIKE"
     gene            10806..11606
                     /locus_tag="PKK03_03425"
     CDS             10806..11606
                     /locus_tag="PKK03_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HNX83476.1"
                     /translation="MKGFVFSIKRYAIHDGPGIRVTVFMKGCPLDCWWCHNPEGKEMG
                     IRMVERIDRVGEKEFRSLEQVGHEYTPEEILAKVQADRVFMEHSGGGVTFSGGEPLMQ
                     PEFLRETLTLLKQAGFHTTVDTSGYVRREILRSIMPVTDLFLYDIKHIDPEIHTKFTG
                     VSNDLIISNLDMLLAEGNDVMIRIPVIPGVSADASYMEQLRGFLENRNNGHIKEINLL
                     PYHKTGSSKYRRFGMDDRMVSATQISNGHLDEYIDILKPLGIHVKKGG"
     gene            11642..14005
                     /locus_tag="PKK03_03430"
     CDS             11642..14005
                     /locus_tag="PKK03_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HNX83477.1"
                     /translation="MTDRVIRLREQSLNATETITAERAQLITRFYATREAQGLSAPVL
                     RARAFEYLLRNKAICINDGELIVGERGPAPKATPTYPEISLHSLQDLNILDSREKVFF
                     RVSDDVKKIYSEEIIPFWKGRSNRDRIMERMTPEWLAAYKAGIFTEFQEQRAPGHTVL
                     GYKMFHTGFLDLKKEIAAAAAALDFRNDPQAYERSEELKAMDIACDAIIMYAGRHADR
                     LEEMASEEKDATRRTELRKMAAVCRRVPANAPETFHEMLQHYWFIHLGVVTELNPWDS
                     FNPGRLDQHLWPVYNKGVESATLTPEEAYELLGCFWVKFNNHPSPPKMGVTAQESNTY
                     TDFCLINLGGVKPDGTNAVNPMTYILLDVIQEMRILQPSSMVQVSRKNPDSFVHKALD
                     IIRTGFGQPSVFNTEAIIQELLRQGKSIVDARNGGASGCVETGAFGTECYWLTGYFNL
                     VKILEVTLNNGFDPRTGVQVGPKTGDPASFSTFDEFMLAYKEQINYFADVKIRGNNVI
                     EKTFAIHLPVPFLSLLLEDCIANGRDYNAGGARYNTTYIQGVGLGSITDILTSIRFNI
                     YDRKRFTWGELLTALQNNFEGAEQMQYDIIYNTPKYGNDDDYADAQAVSVFEFFHDAV
                     SGKPTPRGGIHRINMLPTTSHVYFGNVTGATPDGRKAWVPLSEGISPFQGVDRQGPTA
                     VIKSASKIDHLRTGGTLLNQKFSPEFFEDEGSYQKLTALIRSYFSLDGHHIQFNVVNA
                     DTLREAQKHPELYRDLIVRVAGYSDYFNDLGEDLQNEIIRRTEHQSF"
     gene            14175..14870
                     /locus_tag="PKK03_03435"
     CDS             14175..14870
                     /locus_tag="PKK03_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HNX83478.1"
                     /translation="MKKIFDYFDTRYRADMLGKPTDQSGPVITISRLTGCDGREVAAE
                     LVAQMNLRYHTNRWKWVDKDIIYHAAHELKTDTQRVETFYQGLGMTDISQMIMAFSGS
                     FVTDSSVKKAIREVVLSIARDGYAVIIGRGGVSITRDMANALHVRLVAPLYWRVQNVM
                     KKKGMIIEKAEEYVVNTDEKRQKLIVDFLDKKPQCIDHLFDVTLNRSSFTLEQISSLI
                     LSMYETKLGLKPQ"
     gene            complement(15043..16284)
                     /locus_tag="PKK03_03440"
                     /pseudo
     CDS             complement(15043..16284)
                     /locus_tag="PKK03_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802942.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
     gene            16457..17401
                     /locus_tag="PKK03_03445"
     CDS             16457..17401
                     /locus_tag="PKK03_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNX83479.1"
                     /translation="MNSELQNIAIGVDIGGSHITAVAVDMTAHRIINGSRAESPVDNK
                     AEADAILTVWSDTLKQVLKGMQIFNLRGIGFAMPGPFDYVSGICMIRGVPKYEKLYGV
                     NVGKAIASRLGLPCDCRVRFMNDASSFAVGEAWVGKAAGYRRMMAITLGTGFGSAFLE
                     DRIPVVEGDSVPKMGCVYHLPFGNGIADDYFSTRWFTSRFREVTGREAAGVREVAMMA
                     GRAPEVTEIFSEFGNSLGGFLAPWLKRFGAEILVIGGNISHAFNLFGPALEKTLRNEG
                     CDTKIAISELKEDAALLGSAYLLDNDFWKAVQPALPLM"
     gene            17422..18591
                     /locus_tag="PKK03_03450"
     CDS             17422..18591
                     /locus_tag="PKK03_03450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX83480.1"
                     /translation="MNEKKIEMRLVVPVLLSFFVMSFVDLVGIGKDRVAGDLELSDFI
                     LGLIPFAAFIWFFILSVPVGIIQARVGKKRMLNAGMIITGIGLFIPYFFYNFVTVIAG
                     FALLGIGNTIVQVSANPLLVDVVPGRRASSFLSFSQFIKAIGSMLAAPLAAVFASRFG
                     DWKLLFLVYGIISFLSVLWLGTVKIEETAASGTKASMASAFRLLGNSYILMMVLSIFL
                     VVGIDVGFNYFSGKYLTARFGFEEVSAQSGRSVYFFGRMLGTFAGALILTKLSSTKGF
                     LYSAILSIITILLIMIIPASKAAAWILVFLIGLGVANIFPLVFSLTVQKFPGRSNEIS
                     GLMMMAISGGALIPPVMGLVSDSLGVVPGMGVLLFCAAYLLIVSWIIIRKKMVNI"
     gene            18650..20173
                     /locus_tag="PKK03_03455"
     CDS             18650..20173
                     /locus_tag="PKK03_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNX83481.1"
                     /translation="MKSLIPFALLLLLWSCARQEPVRPNIVYIMTDDHGYQAVSAYGS
                     GLNKTPNLDRIADEGVIFRNSFVCNSICAPSRAALLTGKHSHTNGHIDNRVRFDSSQV
                     TFPKLLQQAGYQTALIGKWHLKSEPTGFDFWSILPGQGSYYNPDFTEMGRTYRQTGYA
                     TTIITDKAIEWLQQRDTAKPFCLLVHHKAPHRNWMPDTMDLDLYDGAVFPVPDNFFDT
                     YEGRQASALQEMGISKDMRMSYDLKITGADGDSLTQSEKDYIWYLDHRMTPEQRTAWL
                     REYDSISREFLAGRPEGDSLALYKLNRYLTDYLRTIESVDRNTGRLLDYLESAGLLQN
                     TLVVYTSDQGFYLGEHGWFDKRFMYEESFRTPLMMRLPSSLSAHGDVSEMVQNIDYAP
                     TFLELAGVKPPADMQGVSLVPLLKGKKQGRWRDALYYHYYEYPAEHAVRRHYGVRTDR
                     YKLIHFYNDIDAWELYDLVADPHEMKNLIDDPAYDKVENHLRKRLDALRKEYNVPEE"
     gene            20191..21459
                     /locus_tag="PKK03_03460"
     CDS             20191..21459
                     /locus_tag="PKK03_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX83482.1"
                     /translation="MSGNSFRRREFIRRGVLAGAALTIVPRHVLGGSGYLAPSDQLTK
                     GIIGVGGMGLGHFSYENTRLLAVCDVDSGHLKRALDKGGKGVKGYNDFRELIARSDID
                     IVHIATPPHWHGIIAKMAAEAGKDIWCEKPMTRTIGEGKRLVEAVKANGRMFRLNTWF
                     RFTGQFYGLGTTVMPLKKVVSSGILGWPLKVTISGITGYDWKFYWSGRTDLLPEPVPS
                     ELDYDMWLGPAPYKPYNKFRVHENFRGYWDYDGGGLGDMGQHYIDPVQYLLEKDHTSP
                     VSVEIDSPQQHYDAVGSWRRITYTYDDGCQIILDGENRDKDAAYIEGPLGKIFKGFVS
                     DIPNLAELVRDLPDPAPQITSFTDSVKTRKPFALNEENGHRSCTIVNMGVVALRLGRN
                     LRFDPVRQLFIDDEAANALLDQPMRGGWHM"
     gene            21479..24745
                     /locus_tag="PKK03_03465"
     CDS             21479..24745
                     /locus_tag="PKK03_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNX83483.1"
                     /translation="MNRIKNLLILTALVLFSTAVSAQDVRTLGTKVADLLARMPADNL
                     ELASRLMEDMYALGDEGRAMICEQVVPAGKGDDLRARYAIASLTAHLSKDSDDARKRA
                     WESQCIQFMKSAGDREVRSFFMRQLSLTGSDMTVSALSDYVSSPEMCDDAVIVLQSVG
                     SEAAASLLYSSLSAGECPCAAQVMVALAERRPGTATDIYMKRYLGGNDAERAAALFAI
                     ASTGAPEAAEILIDAATAASFRRDPTGAVQALLLYAKKIGLSGEVKKMERIASQVIDA
                     SREQEAASQRLAAMSVIAEVKGSDALGMLLEAADDPDLAIRGGVLRLAAGIPGSDVTK
                     KWINRYAKVSDAAKPEILFMLGERGDELAVPLMMKALDDPSHEVSAGAVTALARLQGI
                     RAVDPLISWILKYDSEEGHRAAATALTTVLDSTGMSKIAASLPGSKGNSTVTLIRLLA
                     WSGESSYFSNIIPLTGSDDIAIRAAAISALPALASYDDQTEIIALLDATSDRAEVTEL
                     QRALLSAAMRGDSPGSRSAVILKALDSSRDRLKLIPLLAGTGGEEALKRVAAEFENGD
                     AITRDVCFDALSHWADHSASRALLDITASGNKTFGGPAFDAYMRTVSAAPLTPERKLL
                     LIKDIAPYAAAPDARAGMVAFAASLGLRQVEFFIAPYLSDPVEEVRNAALEALESLKL
                     PEDEVFEPLFNGKDLTGWQGLVGNPLTRARMKPEELAAKQKEANLKMLENWSVRDGMI
                     WFSGRGDNLCSMKEYGDFEMYVDWRITKDGDSGIYLRGSPQVQIWDTSRTDAGAQVGS
                     GGLYNNQKNQSAPVKVADNPVGEWNNFRILMQGENVSVWLNGELVVDNVIMENYWDRS
                     IPIFRKGPIELQAHGTDLAFRDIYVREIKPSEYNLTDKEKEAGFVALFNGKNLDGWIG
                     NKVSYTVEDGMIVIKPGNDSGGNLYTEKEYSDFVFRFEFQLTPGANNGLGIRTPAEGD
                     AAYVGMELQILDNTASIYADLEQYQYHGSVYGVIPARRGFLRPVGEWNYQEVTVQGTK
                     VKVVLNGTVIVDGDIAGAIANGTIDHKEHPGLRNSSGHIGFLGHGSVVKFRNIRIREL
                     "
     gene            24921..25283
                     /locus_tag="PKK03_03470"
     CDS             24921..25283
                     /locus_tag="PKK03_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX83484.1"
                     /translation="MRILICEDNLMVSKAMSVVIEREGYDPVTVSDGNRAIEALQRTD
                     YDLAIVDIHMPYHSGLEVIRYLRTELKKQTPVLIVSAFSDPQIRRQAGEMKVSGYLTK
                     PFDPDDLLKKIRAAVAVV"
     gene            25323..26378
                     /locus_tag="PKK03_03475"
     CDS             25323..26378
                     /locus_tag="PKK03_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HNX83485.1"
                     /translation="MVMLCLTSGLLQSQTVTDPEEEYARIRTLAFEGKYPEAEPAARL
                     LVKQFPEYGDARVLLGRIIAWQGRYDEAAAVIDTLLSSEPDNSDALSALADIRRWSHD
                     RSRQVTAPTDIRAGYMFDTFSEPYERFWQVFTLGAGHRFSWGTAVAAVNYGHINTGPP
                     SGTSDGDFQFAAEAWPELTRKNYAYVSYAYSNGPWFPRHRAALELWQTLPAGLAVSAG
                     VNYYYFDHNIFIGTVSLEKYLAKYWFSGRAYFHFKDIGVTTSFFLNARRYLGTADYVQ
                     LTLGTGTAPDEPYDIITDLERQKASSVRLTWFNQINQWWSFRIGAGYSYEKYSATSNR
                     NRFEGNIGIIRGIGRAK"
     gene            26375..28114
                     /locus_tag="PKK03_03480"
     CDS             26375..28114
                     /locus_tag="PKK03_03480"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNX83486.1"
                     /translation="MKKRLTIVIAFAVTAITLQGFSPAEENGSDSVRSRSAESGLPVL
                     SSFPSELTVGGLVSLASGNYAIQMGAFSRRSNAVALSDKIAGMTGLTLDIIEEGGLFK
                     VFINSSLRGEASCLFIPVAFQGEPKVREPAEEIKQPAVNYPVSPAEASPEPDPEAGMS
                     QDTVSQPANAETESAPATAVIPVEESQSEPQATTTAGTESAGAQNALQKLRNFFLLRG
                     SNPWLTRYNYFGKSVALVNALIITIIASFATMLILLLVILLNRRRMEKEAKLRQYLLE
                     QYQALIIDYLFGSSGPEPFRKIASDNYRRQVLIDQMIDVSVNLKGDSREKLMKLYMDL
                     NLDEDSLERARSRRWHKRIKGFRELAFMGIKEGNDEMYRSLNSRNEILRMEAQIALVR
                     LSEKDHFEFLSHLKRPFSLWEQITLHDLILQHDLPVPDFQRWLSSENPTVVMFALRMI
                     REFRQMEAEPEIRKVLLHREPRVSRLAVEVAGDLGMKTTLETMKRMYKFQEYNNCLEI
                     VKSMGKMPETSMLGFLKLVLDKEDDVQLQIEAVKAIENMGEVGVQALVKVMKSEYKNY
                     NIIVRHVLDRRIY"
     gene            28120..29535
                     /locus_tag="PKK03_03485"
     CDS             28120..29535
                     /locus_tag="PKK03_03485"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX83487.1"
                     /translation="MGFIFAHLIFWLTLVLFATYLLLGIHSALALRKYLRKNSYVNYN
                     SLVLSPLSPRITIIAPAFNEGKTIIDNVRTLLSLYYNNFEVIIVNDGSTDDTFPKIRD
                     AYELVRVNYYFDYRIPCERIRGVYRSKDPAYNRLTVIDKNNGGKADSLNAGINISRSN
                     LIVTIDADSIIEPDSILKLVKPFLEEKEKKVIGTGGVIRIVNSCDVERGHIRQINLPK
                     KFLPRLQVLDYTRAFLLGRMAWSHLDGLMLISGAMGMFDRETVIRAGGYNVKTVGEDM
                     ELVLRMRRYMAEHKQNYEVTYIPDPLCWTEVPADLRSMRKQRTRWTRGLIESLWSHRK
                     MMLNVKYGRLGLLGYPYWLMFEWAAPLIAFLGIIYSIYLAITGALNWPFFLLLFLFVY
                     SFAVSLTTWAVLFEEITFHKYRRKRDVLRLIGTAMLEPFFYPVHTYFAVRGNLESLRG
                     KKGWGKAERSGFQNKKKPATA"
     gene            complement(29641..32226)
                     /locus_tag="PKK03_03490"
     CDS             complement(29641..32226)
                     /locus_tag="PKK03_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX83488.1"
                     /translation="MNARVSFFLNSREFRVSTGTDGRYSVKITGSYSDVAGEFMAGTP
                     YPNPFDNSVNIPFIINTPGDIMLTIYNLAGEKVRIMVFPDVQEGSYNAVWDGCGQNNV
                     PMSAGLYIYAITFRGRSYSGRLVKASGNASGSSGSGLEPVMLPSETPLPGPALRFPVT
                     AEVTAPDYYPVRLTDITLVRDTVINFVLTRRNAMPFTVAGNYIARYIDNDYEPMIFKG
                     VNLGSSPPGYFPGEIAYAISAATYERWINRMGEAGFNSVRIYTLHPPVFYEKLAEYNQ
                     RNPERPLLLFQGIWLEEIEDGTDPLCYDLVSRRSAFSAEIAEVIDCIHGNADIAFRYG
                     KSYGIYRTDISQWTAGYIIGREVAPQEIDSTNKFHPGNASYSGTRFSISGSTASEAFI
                     TEMLDRVVVFEENEYSVSRPVSFSSWPTLDPLEHPTEIFTDEDVASFDITKIARSGGP
                     GLFASYHAYPYYPNFISQDPAYLGFTDGQGANSYLGYLTAMKNHYSNMPLVIAEFGVP
                     SSWGSAHQSYSSMHHGGYSEKQQGQKNMRMMHNMLDAGCAGGFMFAWMDEWFKRTWIV
                     LYQEAYGLNTGGETIPTRQLWHNETSPEQCFGLLAFEQKETPGFVPFSTDQPSGPVSS
                     VRATHDEGFLYVEINTAADLLPTDEFMVAFDTYLGNTGESTLPNGAITGNRAEFLLTF
                     ALDDDTALHHVTEAYDMNGLTVRFNLTDPVQKFKSTTTDGAPWKVMRWINDGFELTYQ
                     DIGKVPLEHASSFTGGDRAAVSWNGRKITIRLPWTMLHFYDPTQMTVNDGAISYDGGY
                     NFEIITAQSDGIAISVWHNGVVTNATNRYSWPKWLTVPQTVEREKASLHIIEEGLSTI
                     PDYVE"
     gene            32508..32891
                     /locus_tag="PKK03_03495"
     CDS             32508..32891
                     /locus_tag="PKK03_03495"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HNX83489.1"
                     /translation="MEKIKIKETTKTPSVSFDPEHGLFEMRGKSIPENSNGFYGPLIQ
                     FVEKYAGSPAERTVLSVRFEFFNTSSSNRIHALFKRFERIFLADRDVLIRWYCENGDE
                     NMRDAGRDFKAILQVPFEIVEVDEL"
     gene            32955..34187
                     /locus_tag="PKK03_03500"
     CDS             32955..34187
                     /locus_tag="PKK03_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNX83490.1"
                     /translation="MALNYIWIAFFLIGFLFALGQLIFNGDTSVFNKIVESTFSSAKT
                     GFEISLGLTGVLTLWMGLMRVGEKGGAVSLLSRVIGPLFRRLFPKLPANSPAHGSMML
                     NVAANMLGLDNAATPMGLKAMKELQEHNPDKEVASDPMIMFLVLNASGLVIIPVTIMV
                     YRAQMGAANPADVFIPILIATYVATLAGLISVAIAQKINLFNRVVLGYLGGLTAIIAG
                     IVWYFSTLPQEQINTISTFAANFILLSIIVSFIVMAMVKKINVYETFIEGAKDGFKTA
                     ITIIPYLVAILVAIGVFRASGAMDLLVDGITAVVAWMGIDTAFTEALPTAFMKPLSGS
                     GSRGMMIDAMTTHGPDSFVGRLACCLQGSTDTVFYVLAVYFGSVGIKNSRYAVICGLT
                     ADIVGTISAIFVAYLFFA"
     gene            34314..35024
                     /locus_tag="PKK03_03505"
     CDS             34314..35024
                     /locus_tag="PKK03_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNX83491.1"
                     /translation="MGRAFEYRRASKEARWDKMSKLFPKLAKAITMAAREGGTDPEMN
                     ARLRTAILNAKAQNMPKDNIKAAIERASGKDTADYAEITYEGKGPHGVLIMVEAATDN
                     NTRTIANIRAYFSKNGGTVVPTGSLEFMFSRKAVFEFDAPENITRDDLELELIEAGLE
                     SLEEYDDTWYVYGDYANFGTLSKALEDKDIEVKKGSLERVALNPVTLTEEQMADVEKL
                     IDRIEDDEDVQAVYTNIA"
BASE COUNT         8482 a         8960 c         9108 g         8513 t
ORIGIN      
        1 catccattac aacactgttc agggaaactg atccgatgtc aagaccgatc agtacggcgg
       61 tcctcccaaa aattgaagtt gcgaccatgg cttaaaaaat taaagatcta ttaccgctaa
      121 tttagatatt tccaagataa tggtttttgc acattaataa taacaatgtg cgtaaatatc
      181 atacaataaa tatgctcctg tgcaaaaaaa gaatcgtccc cgcgaagggg acgattccag
      241 atatctgcca gtttccgtgt ctgccactac cggaggcagg gcagacatca gttatgtgac
      301 atttagttta gcaggatttt catatcctca tctggggtgg tgatgcctgc tattccgaat
      361 ttttccacca ggacattcac gacgttgggt gaaaggaacc ccgggagtgt gggtccgaga
      421 tggatattct tcacgccgag gtagagcagt gcgaggagaa cgattacggc cttctgctcg
      481 taccatgcaa tgttgtatgc tatgggcagt tcgttgatat cgttcttgcc gaagacatcc
      541 ttgagggtaa gggcgatgac tgccagtgag taggagtcgt tgcactgacc tgcgtcaagg
      601 acgcgcggga tgccgttgat gtctcccagt ttcagcttgt tgtagcggta tttggcacat
      661 cctgcagtaa ggataaccgt atctttcggc agtttctgtg cgaactcggt gtagtagtcg
      721 cggctcttca tacgtccgtc gcatccgccc atgacgaaga acttgcggat ggcaccgctc
      781 ttcactgcgt cgacaacctt gtcagcaagc tgaataacct ggttatgtgc aaaaccgccg
      841 atgattgtac cctcttcaat acctgtcggg gccgggcatc tcttcgcatg ttcaatgatg
      901 gctgagaaat ccttttcctt gcctggttcc ctgtcaggga tatgggggca gcccggatag
      961 ccggatgaac cggttgtata aacgcggtca cggtagctgt cgcgaagcgg aacgatgcag
     1021 ttggtggtga acaggaccgg gccgttaaag gtctcgaact cagttgtctg tttccaccat
     1081 gagttgccgt agttgccaac gaaatggctg tatttcttga atgcaggata atagttggcg
     1141 ggcagcatct cactgtgggt gtatacatcc acgcctgtcc cttctgtctg gcggagcaac
     1201 tcctccatat ccttcatgtc atgaccgctg atgaggatgg cggggttgtt tcgtacgccg
     1261 atattgacct ctgatatcac cgggttgcca taggtggtag tgttcgcctt gtcgaggagg
     1321 gccatcactt ccacaccgtg tttgccggtc tccagcacga gagccacaag ctcatcagca
     1381 gaaagagtgt cgtcggtagt ggctgccaga gccttctgga tgaatgcatc aacactgtcg
     1441 ctgttgaatc ccaggttgcc tgcatgttct gcataggctg ccataccctt cacaccgtag
     1501 gttgtcagtt cgcggagtga cctgacatcc tcgtcggggg ttgagaggac gcctacttca
     1561 gcggccttgg cggccaggtc agcctcgctt acgggcttcc aggcaacaca ctcattcagg
     1621 ttgccgggga tggtgattcc gtgagctttc atctcgccgg caaaattatc cctgagagct
     1681 atgccttcat gaatcttttc aatgatcttc accctgtcaa agttggcatt ggtaattgtg
     1741 gtgaagagag cttcctgcac aaagcggtca acctttgcat tggaatagtg gttctgacgt
     1801 gcaatggtac caaggattga tattcccttg gcaatccaca ccagcatgtc ctgcatctgg
     1861 gctacatcat cagtcttccc gcatacgcct tttacgacgc atccggttcc ttttgccgtc
     1921 tcctggcact ggtaacaaaa catactcatc tcttgaattt ttttgggttt atattcaggt
     1981 ttgtactttt attctacatc cattcctcct gcagtatctt accttcgata ctcactacga
     2041 ctgttttgac gggtaccttg cgtgaagccc tggcgagtgc ggccctgacc atctgcaaca
     2101 gtccgccgca gcagggaacc tccatcatca gcacggtgat ggtgttgact tttgctgtat
     2161 ctatcatgac agtcagttta tctatatatg tttccgttcc gtgatcaagc ttggggcatg
     2221 ctattgcaag cgctttcccc ttcaggaaac ggctgtggaa tgagcccatg gagaatgcta
     2281 cacagtctgc tgccagcagc aggtcagctc ccctgtatgc gggtgagttg gggttaacca
     2341 ggtgcatctg cacaggccag tgggtgagca tggaaggtga ttctgccgat gctgttccga
     2401 agacgggcgc tggtgctatc agcctctcag cgctgcccgg gcatccaccg ccatgatgcc
     2461 ggtgcccgtg ctggtgttgc tgatgctggt gctgaagatg agcccctcct gctgcctggg
     2521 cctgcccttg cttaccgctg actgcatcaa gaacctcagc agcatcaaat gacagctcac
     2581 tgccatgaac ctcaagccag gtggttcctt cctccaggta accgtactcg tggtggtctt
     2641 tcagatgctt cagatgggct ataacagtat tcggaccctt ctttaccatt tcactgataa
     2701 cagcgatctc gtcatatggc tcagcctcac gcttttcgat agtgatggcg ccttccgggc
     2761 agtgtccgat acacgccccg agcccgtcac acatcagctc agataccagc cttaccttgc
     2821 cgtcaatcac ctgaagagca ccttcgtggc agttgggtac acaaacgccg catccgttgc
     2881 atttctcctc atctatctta ataatgtctc tttgcatttt attgtctcag ttttatgatg
     2941 caaaggtatc ctcatcactg attgtggttt gtaacatatg ttacaattta cgaattattt
     3001 ctacatttaa ccaaaaaaag agattaatgc gttttccggt actgagtaat tccccgctgt
     3061 tcaggggact gactgattca gaaatagaaa agctgatagc tgcagtcaat ttcagggtaa
     3121 ggttttatca tgcaggagct gttgccgcac tcgcaggtga agagataacc tcactgatga
     3181 tagtgctttc aggcagcgta aggggagaaa tgtcagatct ttcaggacgc accatgaaaa
     3241 ttgaggatat aaatccgcct caggcgcttg ctgctgccgt gctcttcggt gaaggagcga
     3301 ggtacccggt gaccgtgata gccaattctg attccgagtt actgattatc aacaaggacg
     3361 attaccttgc aatgatggcc ggtaacaaac ggatactgtc aaactacctc tcgtttatct
     3421 gcggtaaggc acagttcctt tcgggccgtc tcaggtttca ttcattccat acaatcaagg
     3481 ggaagtttgc acattatatt tcttcgctgc caggcgcttc aacaggccgt gtggtcatag
     3541 accgcccgca gcaggagctt tcagagtatt tcggagtgac acgtccttca cttgccaggg
     3601 caatcggtga gatggagcag gagggactta tttccgttga ccgcagggag gttaggtttc
     3661 tcaatctgaa agggctgtca gcgcttaccg gagcctgaat gcctggtcag agccgggcag
     3721 tatcacggta aagcaggtac cgctgccggg agtgctctcc acggtgatgg tccccttgct
     3781 caatgccacc agctcataaa cgagcctcag gcctatcccg gttccctttt cggaatcggt
     3841 gcctggcgtt gactcaatct catgcccggt gaagagcctc ttctgacgtt cgggagtgat
     3901 cccgaccccg ttgtcacaga tccttatggt cacccattcg gaagactctt ccctctcctt
     3961 cacatgaact gtcacctttc cgccgtgata tgtatactta attgcatttg tgagaagatt
     4021 ccggattatt atgtcaacaa ggtccctgtc aaaccagccg attgatctgc caacctgggt
     4081 gaaattaagg tcaatgtttt tcctgtcagc gctgtcttta aggatgctga tgttggtcag
     4141 gattatgtca gcgaggtcat tctcggcagg agcatacttt atcatgtctt cctggcccct
     4201 tccccagacc agcatgttct cgaggaggct gatgagttgc tgggagtact gaatgcatga
     4261 gtctgcaagt gattctgtcc ggtcacggaa ctcctcctcc ttcagcaggt agagggtgta
     4321 gaggatgttc accacgggac ttcgcaggtc atgtgctatc acagaaaaaa tcttgttctt
     4381 caggcttatc gtctcggtga gctttttatt ctgcagggcg atctgctgat tccttgcatt
     4441 aagctcttct gtgcgatccg tgagctccct ggtgcgttct tcaaccctgc tctcgagact
     4501 gtggttcagt tcctcgagtt gccccgagag tttctccctc tccttgcccc ttcttaccca
     4561 gtcagctatc agaagggttg cctgaatcag cacgaagatg aggatcagga atgacaggat
     4621 gtaaagctgc tggttctctt ccagtgcgtt tgacataaga atgtcagata ccaatccgaa
     4681 taagatggcc aggaatgcca gaaggtacag gatgtctctc cggttgcctt tcatgatgcc
     4741 cctgatgctc atcgagacta cgtaaagcat agctaacatt gcgatagcct gtatgccgaa
     4801 gactgaccat gaaaagatcc ttaccggaag gaaaacaacg gcagctaccc cgggaacaaa
     4861 aacaacatct gcggcaattg caaacctcct gaaccacctg gcgggataga tcaggtatgc
     4921 cagccacatc ccggaggtaa ccatgaataa cagtataaga tattctcccc ggatgatgag
     4981 gttccagttt cttatgtcaa caattgttgc aaggtacggg gctgagagaa agggacggag
     5041 ggcaaggcag aggaccagta cggcaaacat cagcagcttc ctgtcacgcc tgtcaagcac
     5101 gtagaaaatc agaaagaaca gaaatgaagc gaagaggatg cctgatacca ggatagagat
     5161 aaaccactgg tttgtgaagt tggtctgtat tgagtgaaag gtgcctgcct tcataggcat
     5221 ccagaatccc ccgcgccggt gttcatagtt ggagaccctg atcagcagtt cgagggtgtc
     5281 acttttgggt acataactga agaagagggg ttcgtaggag gggaccgact cagccctggt
     5341 tcgtgcggga gttccgttcc tggccatggt gacgccattg atggttatct cgtacgaggt
     5401 atcaaacacc ggcatgtcaa atcccatcct gtcacggtat ccgcggggca gcaagatcag
     5461 ggcccggtat gtggcgtacc cgaaccgggg aagtttcctg ccgtcgacgg tgtaatcgct
     5521 ccagtaaccg ggaaccgtgg cataacagtc cggaatcagt gtgtcacaaa tatctccggg
     5581 tgtgccattg atgaatgtgc cgaaatagaa ctcccactct ccgttcatcc ttacggtgaa
     5641 ctcgtctccc tgttgtatct tactcaggtc aagcacgccc ttctcaaccc tgggtgataa
     5701 ggcagcaacg cttcctgata aaagtatcag caagaggaaa gtcatagaga cccttcccgg
     5761 cacacgcatc ccggcattca caggctaccg gtttataaat ccccggtttt caagcagagc
     5821 ctctatgcct ggctcacggc ccctgaaggc aatccacatc tcaagctcat cctttgatcc
     5881 cttccgtgaa aggatctcct tttcgaagcg ggctgcaacc tccttgtcga agatgtcccc
     5941 cgtctcctta aaggctttga aggcgtcgga gtcaagctgc tcacaccaga tgtagctgta
     6001 gtaaccggcc gaataaccgc caatgatgtg gttgaaataa gttgacctgt agcggggaat
     6061 aatctcgctg atcagtccgt acttttccat ggccttcttt tcaaattctc cgatgttcag
     6121 ttcgacaggc tctgtcaggg agtggtattc catatcgagt gatgaggcag ccaggtactc
     6181 gatggtagtg aatccctggt tgaacttgcc ggccttctct atcttttcta tcagtgcgtc
     6241 agggatgacc tcaccggttt gatagtgttt tgcatatacc ttcagcacct ccggttcaaa
     6301 gacccagtgt tccataacct gtgaaggcag ctccacgaaa tcacgtgaca caccgctggt
     6361 atactccgtg tttgacagaa ggttgtgcag ggcatgaccg aactcatgga agagtgtgct
     6421 tgcctcatcg ggggtcagaa gtgacggagt atcgccagaa ggaggtgtga aatttgtgac
     6481 catgctaacc agcggcgtca cgaatttgcc attttcgtca atacgctggg gcctcatgcc
     6541 gctgcaccat gcaccgcccc gcttcgaagc cctcggatga tagtcgatca taaggatgcc
     6601 aacgtgcttg ttgtcacgct tcacctccca tgtctttaca tcaggatggt acaggggtat
     6661 gtcgttcctc tcactgaatt ccagtccgta aagcctgttg gcgacataga acattccgtc
     6721 agtgacattc ctggctgaga aatagggttt cgtctcttca tcgctcaggt cgtacttctc
     6781 cttgcggatt ttttcggtgt agtagaacca gtcccatggc tcgagtttga atttgccgct
     6841 ttccctgtca ataagctcct gctgcgctgc agcctcggcc ttcgccaccg gaagggcagc
     6901 ctcccatatc tgtgagagga aattaaggac tgtctcaggc tcctttgcca tgttgcgctc
     6961 aaggacatag tcggcatgac tctcatatcc cagcagcctg gccttttcta cccgcagcct
     7021 gattatgtcg gccaggatct tgttgttgtc atgttcgttc ccgttgttgc cccgcatgct
     7081 gtaacccctg aacatcttct ccctgagggc cctgttgtct gcataggtca tgaagggaat
     7141 gtagctggga ttttgcaggg tgaagagcca tttaccctcc atattctttg ccctggcagc
     7201 tgcagcagcc tgtgccctga tcccttcggg aataccagag agatcctgtt cattgtcgat
     7261 gacgagggtg aaagcattgg tttcagccag caggttttcg ccgaacctga cacccagcag
     7321 tgacagttca ctgtttaatt tgcggagtac ttccttgttt tcggaagaca ggcctatgcc
     7381 gctcctgatg aaaccgaggt attcgtcttc aagaagtttg ctctcctcgg gtgtgagatt
     7441 gaatttctca cggttttcat atactgactt aactctttca aagagctttt cgttgagggc
     7501 aatatcgtcg ctgtgcctgg agaaaagcgg actcatctcc ctctgcagcg cctgaatcga
     7561 gtcattggta ttggcgccgg aaagcggacc ccaggcacgc tgtacctttt gcaacagttt
     7621 gccggtgaac tgcatggccc tgatggtatt gtcaaaagta ggctcgtcag tgttgctgat
     7681 gatggcattg acctctgcca tctcttcttc catccctttc aggaaggccg gcttgaaatg
     7741 ttcaattttt atcttgtcaa acgggggaac ttcaaaaggt gtgtcccagt cgcaatagaa
     7801 aggattctcg cagctctcct tcttgcagga aataactccg agagaggcaa tgaacatcat
     7861 gataaggatt tttttcatta cggttggtgt ttgggttcgg ttggctaaaa tatggattaa
     7921 tgccttaaat tacaattgaa cgggaagtat ctgccctgtt ttgcagcata gctgcgggaa
     7981 acgggctaac tgaattcaat cagcgggtct cccttgctga tcttgtcgcc gctccttaca
     8041 aggattttct tcaccgtgcc tgcacggtca gataccacct ccctgatggt ttttcctatc
     8101 attatgtaca taaggatctc ccccttttta actttctctc caacgttcag catctcctca
     8161 tcgggccagt aggttgccgt tgcccccgag tagaggaatg ctatgcctgc ttcgggtgaa
     8221 tgcaggagtt tgcgtggcgg cgttttcgcg ggagttccct gtgataactc agcctgtttg
     8281 tcagcgatct ccttcaggaa ccttttctgt gccacaccgg atttgtaatc acggtattgc
     8341 ctgtcatgca tcgccagttc gaagagttcc tcctcatcgg gccctgcgga ccacccgttg
     8401 tcttccatct ctttccggta ggtatcgagt gcatcaggat agttttcctg tggattccct
     8461 tcatagaact ccttccctgc tttcccggcc agttcaacca gttcaggtgc cagcggaccg
     8521 ggaagagttc cggcacgtcc cagcagcata tcccaggtgt taggatcaat cattgcaaat
     8581 ctttccttgc cgttgaccag agagacgatg ttcataagag ccacattctt aacatactgg
     8641 ctgaatggtg tgaccagcgg aggataaccc atcatcggcc agatatgctc cacctccctg
     8701 aagagctgca ccaccagttc atcctcggtc atttcaggct tgcttctggc tttcagggcg
     8761 gcattgattc cctggtgaac ccccttcagg tctgccatca tgctccccat cattccgccg
     8821 gggaggcctg acccgataag gagggaggtc atctgcctgt tgcgcggatc aatgaaatag
     8881 ccaagaaaat catttatgaa ttcctgtgtc agcgccctta ccttcatgta ggcgtccatg
     8941 ttgatctcag gtacgtcaaa cccggcgtcg ctcatcaccg cgtgcaccgc aatcacgtcg
     9001 gggtggacca tgccccagct gagcggctcc attgccacat cgaggtaatc agcgccggct
     9061 cttgctacct caagcattga ggccaccgac attcccggcc cgctgtgacc atggtactgc
     9121 acaataatgc cggggtattt gctcttgatc cttcttacga gctcaccgag ggaagcgggc
     9181 cggccaaccc ctgccatatc cttgagacag atctcgtcag cgccgtactc cacgacatgg
     9241 tcaacaatac ccatatagta atcgacggta tgaactggcg agtgtgtaat agagagggct
     9301 gcctgtgata tcatccctgc ctcgcgggca tatttgactg acagctccag gttacggtga
     9361 tcattcagcc cgcagaacga tcgcgcaata tcaactccct gcgctttctt gaccctgtac
     9421 atcagcttcc tcacatcagc cggcaccggg tacatcctca aaccattgag ggccctctca
     9481 agcatgtgtg tctgaattcc tgccttgttg aatggtttca cccattctct tacagccgta
     9541 ttcggattct cgccgtaaag cagttgcacc tgctcaaatg cacctccatt ggtctctacg
     9601 cggtcaaaac aacccatctc aactatatac ggcgcaacgg cagtcagctg gtctaccctc
     9661 ggctggtatt tgcctgatga ttgccacatg tcacggtaca ggaggctgaa ccttatcttc
     9721 tgtttcattg tcttttgtat cagtagctgc ccgcgaacca gggggcggca ttacaacatc
     9781 aaagataatc cggtttggct ctgcaaaatt gattattgtc attaatataa aagaagaaga
     9841 tacccgcttg gggtatcttc aattcaacct aaacctgatc gtagacaggc cggacggcct
     9901 gattaaggaa ggaaaaaatc tttcttccgg cagctattcc ttgataagct ccgggaaata
     9961 cgatgcaatc agcatggttg catcaaattc ttctgtggcg ccctgggaaa tgaccttctg
    10021 atgaaagtag tcatacctgt cgagcttctc atcggtcgtt gccagaccgg cagccctgta
    10081 cttctcgcca agccggctta caccgaagaa ttcgccgttg caagtgtaca tgactgaaag
    10141 ttcgtcggtg atgacaaggt aattcttgca cttcttctcc ttgtcaacca gcaccttaac
    10201 ctgtaaaggt gatttttcat aactgatgag gtagcatttc atctcctcac ctgccagcat
    10261 tacagtctca tccagacgct caatggtgta ggctccgaag ggagtgttcg attcgcccct
    10321 tatgatcacc ttgccgaagg agagcagtga gaatgctgca aacaacacca gaaccaatga
    10381 aattcttttc atgaccaacc cgtttttgtt aaacacctaa ctgtcctgta agaaagcgga
    10441 actgtccttt tccatcttct ttccgttata aagaccgtca acacccccta ttgttgcatg
    10501 gattatttgt taaacttttg ttaacccact ggataattct gtaatcgata cattttcagc
    10561 gctatggcga tgcgaattaa attccgccat caccgccggt gccttcccgc ttggaaaatc
    10621 cagaatcatt tcttaccttt gtgttccata ttaagggtat gaacagactg gaacacaggt
    10681 ttatgatgaa ccgcctcggc agaaggctgt atcattgcct tatgttctat gcccgataac
    10741 ctttcatgag gggctcccga agccctgcct cccactattt gttacccgaa atccgttatc
    10801 agattatgaa aggttttgta ttcagtataa aacgatatgc aattcatgac ggcccaggca
    10861 tcagggtcac tgtcttcatg aaagggtgcc cgctcgattg ctggtggtgt cacaatcccg
    10921 agggaaagga gatgggtatc cgtatggttg aaaggattga cagggtggga gagaaggaat
    10981 tcagatcatt ggagcaggtg ggacatgagt acaccccgga agagattctt gcaaaggtgc
    11041 aggcagaccg ggtcttcatg gaacactccg gaggaggcgt caccttctca ggcggcgaac
    11101 cactgatgca gccggaattt ctccgtgaga ccctcaccct tctcaaacag gcaggcttcc
    11161 atacaaccgt tgacacctca ggttatgtgc gccgtgagat actacgttca atcatgcctg
    11221 tcaccgatct tttcctttat gacatcaagc atattgatcc ggagatccac acgaagttca
    11281 cgggcgtgag caatgacctg ataatctcta accttgacat gctcctggct gagggaaacg
    11341 atgtgatgat acgaatcccg gtcataccgg gagtatcagc cgatgcttca tatatggagc
    11401 agctccgcgg ttttcttgaa aaccgcaata acggacacat aaaggagata aacctgttgc
    11461 cctaccacaa gacgggcagc tcaaagtacc gccgtttcgg aatggatgac aggatggttt
    11521 ccgccaccca gatttcaaac ggccatcttg acgaatacat tgacatatta aaaccgctcg
    11581 ggatacacgt taaaaagggc ggatgaaaac agaataaggc agcaaagatt ttactatgaa
    11641 aatgacagac agagttatta ggctcaggga acagagcctg aacgcaactg agacaataac
    11701 ggcggagaga gcacagctaa tcacccgctt ctacgccacc agggaggccc agggcctttc
    11761 ggcacctgtc ctcagggcac gtgcctttga atacctgctg aggaacaagg ccatttgcat
    11821 caatgacggt gaacttatcg tgggcgaaag aggacctgca cccaaggcta ccccgaccta
    11881 cccggagata agccttcact cactgcagga tctcaatatt ctcgacagcc gcgaaaaggt
    11941 cttcttcagg gttagcgatg atgttaagaa gatatacagc gaggagatca ttcccttctg
    12001 gaaggggagg agcaaccgtg accgcattat ggagcgcatg acccctgagt ggctggccgc
    12061 ctataaagca ggtattttca ccgagttcca ggagcaaagg gcgcccgggc atacagttct
    12121 gggttataag atgttccata caggattcct ggacctgaaa aaagagattg cagctgctgc
    12181 cgccgcactt gatttccgta atgacccgca ggcctatgag cgcagcgagg aactgaaggc
    12241 catggatata gcctgtgatg cgataataat gtatgccgga aggcatgccg acaggcttga
    12301 agagatggct tcggaggaga aggatgctac aaggcgcaca gagcttcgga agatggctgc
    12361 cgtatgccgc cgcgtgccgg ccaacgctcc ggagacattc catgagatgt tgcaacatta
    12421 ctggttcatc cacctgggtg tggtaaccga actcaatccg tgggactcat tcaaccctgg
    12481 caggctcgac cagcacctgt ggccggtcta taacaagggt gttgagagtg caaccctgac
    12541 tcctgaggag gcttatgagc tcctcggatg cttctgggta aaattcaata accatccctc
    12601 tcccccgaag atgggtgtca ctgcacagga gagcaacacc tataccgact tctgcctgat
    12661 aaacctggga ggagtgaagc ctgacggtac caatgcggtc aatcccatga cctatattct
    12721 cctcgatgta atccaggaga tgcggatact tcaacccagc tcaatggttc aggtaagccg
    12781 caaaaacccg gactcattcg tgcacaaggc actagatatc atcaggaccg gtttcggaca
    12841 gccttcagtc ttcaataccg aggcaatcat ccaggaactg ctgaggcagg gcaagagcat
    12901 agttgatgcc cgcaacggag gagcatcggg ttgtgttgag accggagcct tcgggaccga
    12961 gtgctactgg cttaccggat acttcaacct tgtgaagatc cttgaggtca ccctcaacaa
    13021 cggattcgat ccccgcacag gcgtacaggt gggaccaaag accggcgacc ccgcgtcatt
    13081 ttccaccttc gatgagttca tgctggcata caaagagcag atcaactact ttgcagatgt
    13141 caagataagg ggcaataatg tcattgaaaa gaccttcgcc attcacctgc ctgttccgtt
    13201 cctgtcactc ctgcttgagg actgcatagc aaacggcagg gattacaatg ccggcggagc
    13261 caggtataat accacatata tacagggagt gggtcttgga agtatcactg acatactcac
    13321 ctcaatcagg ttcaatatct atgacaggaa gaggttcacc tggggtgagc tgcttaccgc
    13381 actgcagaac aacttcgagg gagcggagca gatgcagtat gatattattt acaacacccc
    13441 gaaatacgga aatgatgatg actatgctga tgctcaggca gtatcggtat ttgagttctt
    13501 ccatgatgct gtcagcggca agccgacacc cagaggaggc atacaccgca tcaacatgct
    13561 cccgaccaca tcacatgtgt acttcggcaa tgttaccggt gcaacgcctg acgggcgaaa
    13621 ggcatgggtt cccctgtcag aaggcatatc cccgttccag ggcgttgacc gccagggacc
    13681 cactgccgtg atcaagtcgg cttcaaagat tgaccatctc aggaccggcg gaacactgct
    13741 taaccaaaag ttttcaccgg agttcttcga ggatgaagga agctaccaga agctgaccgc
    13801 ccttatcagg agttacttca gccttgacgg ccaccacatt cagttcaacg ttgtcaacgc
    13861 tgacaccctc cgtgaggctc agaaacaccc tgagctttac cgtgacctga ttgtacgcgt
    13921 ggcaggctac agtgattatt tcaatgacct gggagaggat cttcagaacg aaatcatccg
    13981 acgtaccgaa caccagagct tctgacggaa tcgtccgcag cgcctgcgtg accttgccaa
    14041 aatgctgccg gaaatcaatt tttattggtt tccggttgcg ttttactaaa ttatttacta
    14101 tattttgtaa gaattagatg ctgcagtatt caggcagcaa cgcacagaaa gatgcaacac
    14161 ctaaggcctt ggccgtgaag aagatatttg attatttcga cacccgctac agggctgaca
    14221 tgctcggtaa gcccactgac cagagtggcc cggtcataac tatctcgcgc ctgaccgggt
    14281 gtgacggccg tgaggtggct gccgaactgg tggcccagat gaatctgagg taccacacca
    14341 acaggtggaa atgggtagac aaagacataa tatatcatgc cgcgcatgag ctgaagactg
    14401 acacccagcg tgtggagacc ttttaccagg gactgggaat gaccgacatt tcccagatga
    14461 taatggcatt ctcagggtcc ttcgtgacag acagctcggt gaaaaaagcc atcagggaag
    14521 tggttctctc aatcgcccgt gacgggtatg ccgtgataat tggcagggga ggtgtctcta
    14581 tcacacgtga tatggccaac gccctgcatg tacggctggt tgctcccctt tactggaggg
    14641 tgcagaatgt gatgaaaaag aaaggaatga tcattgaaaa ggccgaggag tatgttgtca
    14701 atacagacga aaagcggcag aagctgattg ttgatttcct cgacaagaaa ccccagtgca
    14761 tagatcatct cttcgatgta actcttaaca ggagcagttt tacccttgaa cagatctcat
    14821 ctctgatatt gtcaatgtat gagacgaagc ttgggctgaa acctcaatga taaagctgtg
    14881 tgttttcaga gtttgacaca gctggctggt tgattaacag gagggtaagt gaaagggccg
    14941 gataacgatg gttaccggcc ctttctgaat cctgcatcag agacagcaga tgtcagagat
    15001 gcgttttata tgctccattg tccggctgtc aagtttcctg acataggcat cccagtcttc
    15061 aatgaggaag cgtgccactc cggtttctat ggctgcgcgg gctactgcgg gcgcaaccct
    15121 tgagataaga cgcggatcca ggggttttgg tattatgtat tccctgccga attccaggtt
    15181 ttcaaggcca tatgccttca gcactgcctc cggaatcttc tccttggcaa gcccggcaag
    15241 cgcacgcgtc gcagcaagct tcatctcctc gttgatggtc ctggccctga catcaagagc
    15301 acccctgaag atgaagggga aaccgaggac gttattgatc tggttgggat aatcagacct
    15361 gccggtggca aagatcaggt catcacgggt tgccatggca tcctcgtaac ttatctccgg
    15421 attgggattg gccattgcaa agactatcgg attgggagcc attgtactca gcatctcact
    15481 ggtcagcatg ttggccacag agaggccgag gaatacatca gcccctttga ctgcctctgc
    15541 aagtgtgctg atgttcaggt cggtaaccca ctctttcttg tatttgttca gatcggttct
    15601 ccgggtagtg ataacgccct tgctgtcaac cattatgaca ttttcctttt tcactcccag
    15661 cgccacatag agcctggtgc aagagttggc cgcggcaccc gccccgcaca ccaccacctt
    15721 aaggtctgag atattcttgc cgaagatctc aacggcattc agcagccctg cggcagagat
    15781 gattgccgta ccgtgctgat catcatgaaa caccgggatg tcaagcatct ccttgagcct
    15841 tgtctctacc tcgaagcact ctggcgcctt gatgtcctcc aggtttatcc cgccgaaggt
    15901 gggagccagt ttcgcacata tctctatcag cttctccggg tccttctcgt caatctcaat
    15961 atcaaacaca tccacgtctg caaatacctt gaacaggagt cctttgcctt ccatcaccgg
    16021 cttaccggcc agcgcaccta tgtcgcccag gcccagaacg gctgtaccgt tggagataac
    16081 cgcaaccagg ttgcctttgg cagtatacat gtatgcatca tccgggttct tctctatctc
    16141 aaggcaggga taggccactc cgggagtata ggccaggctg agatcaaact gggtacagta
    16201 aggcttggta gggatcactt ccaccttacc cttgcggccg cgactgtgat acagaagcgc
    16261 atcctcttta gtggcttttg gcatgacaat atgagatttg attgtgaacg gttcggaata
    16321 gcacgattac tattacgaaa ttaagacatt tctcagactg atcctatttt ttcagaataa
    16381 tatgccggat tccttttgca ctctttggga aaatagatat tttcattgct ttaaaaagga
    16441 acctgaggag accacaatga attctgagct tcagaacatc gccattggcg tggatattgg
    16501 cggcagtcac atcactgccg tcgccgttga tatgactgcc caccgcatca taaacggctc
    16561 gagggctgag agcccggttg acaacaaggc ggaagccgat gcaattttaa ccgtctggag
    16621 tgataccctg aagcaggtgc tgaaggggat gcagatcttc aatctccgtg gaatcgggtt
    16681 cgccatgccg ggcccgtttg actatgtcag cggcatctgc atgataaggg gtgttcccaa
    16741 atacgaaaag ctttacgggg tcaatgtcgg caaagccatt gcctcaaggc tcgggctgcc
    16801 atgtgactgc cgggtgaggt tcatgaatga tgcaagctca tttgccgtgg gcgaagcctg
    16861 ggtcggaaaa gctgcaggct accgcaggat gatggccatc accctcggca ccgggttcgg
    16921 ctcagctttc ctggaagatc gtatccctgt tgtggaagga gacagtgtgc cgaaaatggg
    16981 ctgtgtctac cacctgcctt tcggcaacgg aatagctgac gactacttct cgacccgctg
    17041 gttcacttcc cgcttccggg aggtgacagg ccgcgaggct gccggcgtaa gagaggtagc
    17101 catgatggct ggaagagccc cggaggtaac ggaaatattc agtgaattcg ggaacagcct
    17161 ggggggattc ctggcgccgt ggctgaaaag attcggtgct gagatcctgg tcatcggcgg
    17221 aaacatctcg catgccttca acctcttcgg ccctgccttg gagaaaactc tgaggaatga
    17281 gggatgtgac accaaaattg caatctcgga actgaaggag gatgctgccc tgctgggaag
    17341 cgcctatctc cttgacaatg atttctggaa ggcagtacag cctgctcttc cgctgatgta
    17401 agactgaaca acctgaacaa tatgaatgag aaaaaaatcg agatgagact ggtggtgccg
    17461 gtgctcctct ccttcttcgt tatgagcttt gttgacctgg tgggtattgg caaggaccgg
    17521 gtggcaggcg accttgaact gagcgatttc atccttggtc ttattccctt cgcagctttc
    17581 atctggttct tcatcctgtc tgttccagtc ggaataattc aggcccgcgt aggaaaaaag
    17641 agaatgctca atgccgggat gatcatcaca ggcatcgggc tgttcattcc atatttcttc
    17701 tacaacttcg tgactgtcat cgctggcttc gcccttctgg gcatcggaaa cacaatagtc
    17761 caggtctcgg ccaatccact tcttgttgac gtggtgcctg ggcgcagggc atcaagcttc
    17821 ctcagcttct ctcagttcat caaagccatc ggctcgatgc ttgcagcacc gctggcagct
    17881 gtcttcgcaa gcagattcgg cgactggaaa ctgctgttcc tggtatatgg cataatctcc
    17941 ttcctcagcg tactctggct tggaaccgtg aagatagaag agaccgccgc atcgggcaca
    18001 aaggcatcaa tggcttcagc cttcaggctg ctcggcaact catacatcct catgatggtg
    18061 ctctcaatct ttctcgtggt cgggattgac gtgggattca actacttctc gggtaaatac
    18121 cttaccgcca ggttcggctt cgaagaggtg tcggcccagt cgggccgaag cgtttacttc
    18181 ttcggacgca tgctcggcac cttcgccgga gctctcatcc tgacaaaact ctcttccaca
    18241 aaaggtttcc tttacagcgc catactctca ataatcacca tcctgctgat tatgattata
    18301 ccggcatcaa aagctgctgc ctggatcctt gtcttcctga tcgggctggg tgtggcaaat
    18361 atcttcccgc ttgtcttctc tctcacggtc cagaagttcc ccgggagaag caacgaaatc
    18421 tccggactga tgatgatggc catctccgga ggggctctca taccaccggt gatgggactg
    18481 gtgagcgact cgctcggcgt ggtgccggga atgggtgtgc tgctgttctg tgcggcatac
    18541 ctgctgattg tgtcctggat cattatcagg aagaaaatgg ttaacatctg attcaaaggc
    18601 ctgaaaagga tatgtctctt ttgattaaca tgcggtaatt ttaactatca tgaaaagcct
    18661 catcccattt gccctgcttc tcctcctctg gtcatgtgcc aggcaggagc cggtcagacc
    18721 caacatcgtc tatatcatga cagatgatca tggttaccag gcggtgagtg catacggaag
    18781 cggacttaac aaaacaccaa atcttgaccg tattgcggac gagggggtca tcttccgcaa
    18841 cagcttcgtg tgcaattcaa tctgtgcgcc cagcagggct gcactgctga ccgggaaaca
    18901 cagccatacc aatggccaca ttgacaacag ggtcaggttt gacagctctc aggtcacatt
    18961 ccccaaactg ctgcaacagg ccggatacca gacagccctc atcgggaagt ggcacctcaa
    19021 gagcgaaccg acgggattcg acttctggag catactgccc ggtcagggaa gctattacaa
    19081 tcctgacttc acagagatgg gacgcaccta ccggcagacg ggctatgcca ccaccatcat
    19141 caccgataaa gccatagaat ggcttcagca gagagacact gcaaaaccct tctgcctgct
    19201 ggtgcatcac aaggcacccc accgcaactg gatgccagac acgatggacc ttgatcttta
    19261 tgacggcgct gtgttccctg tgcctgacaa tttctttgac acctatgagg gcagacaggc
    19321 ttccgccctc caggagatgg gcatcagcaa ggatatgcgc atgtcatacg atctgaagat
    19381 cacaggcgct gacggcgact cactgacaca gtcagaaaag gattacatct ggtaccttga
    19441 tcaccgtatg acccctgaac aacggaccgc ctggctcagg gagtatgaca gcatcagcag
    19501 ggaatttctg gccggcaggc ctgaaggaga ctccctggcc ctctacaagc tcaacaggta
    19561 tcttactgac tacctccgta ccatagagtc tgttgaccgt aacaccggaa ggctgcttga
    19621 ctacctcgag tcagccggac tgcttcaaaa cacactcgta gtctatacct ctgaccaggg
    19681 tttttatctt ggcgaacatg gctggtttga caagcgtttc atgtacgagg agtcgttccg
    19741 cacaccgctg atgatgaggc tcccttcatc actgtcagcc catggtgatg taagcgaaat
    19801 ggtgcagaac attgactatg caccaacgtt tcttgagctg gccggggtaa agcctccggc
    19861 tgacatgcag ggagtgtcac tcgttcctct gctcaagggc aaaaagcagg ggaggtggcg
    19921 tgatgcactc tattaccatt actacgaata tcctgccgag catgcagtga ggaggcatta
    19981 cggagttcgt accgacaggt acaaactgat ccatttctat aatgacatcg atgcatggga
    20041 actctatgac ctcgttgctg accctcatga gatgaagaac ctgatcgatg atcctgccta
    20101 tgacaaggtg gaaaaccacc tcaggaagag acttgatgcg ctcaggaaag aatataatgt
    20161 cccggaagaa taatgtaaaa agaaaatatc atgtcaggaa attcattcag gagaagagaa
    20221 ttcatcagga gaggggtgct ggccggtgct gctctgacaa tcgttccgcg tcatgtgctc
    20281 ggtggcagcg gctacctggc gccgagtgat cagcttacca agggtatcat tggcgtggga
    20341 gggatgggtc ttggccattt cagctatgag aacacccgtc ttcttgcggt atgcgacgtt
    20401 gactccggtc acctgaaaag agctctcgat aagggtggta aaggggttaa gggttataat
    20461 gatttccgcg agctgattgc aaggtctgat attgacattg tgcatatagc cacacctccg
    20521 cactggcacg gcatcatagc aaaaatggcc gctgaggccg gaaaggacat ctggtgcgag
    20581 aaacccatga cccgcacaat cggtgagggg aagaggctgg ttgaagctgt gaaggccaac
    20641 ggaaggatgt tcaggctcaa tacctggttc cggtttacag gccagttcta cggcctcgga
    20701 accactgtca tgcccctgaa gaaagttgtc agcagcggca tacttggatg gccattgaaa
    20761 gtaacaatca gcggtatcac cggctacgac tggaagttct actggagtgg caggaccgac
    20821 cttctgccgg agcctgtgcc ttcagagctc gattatgaca tgtggctggg ccctgcacca
    20881 tacaaaccat acaacaaatt cagggtgcac gaaaacttcc gcggatactg ggattatgac
    20941 ggcggaggcc ttggcgacat ggggcagcac tacattgatc ccgtccagta cctgcttgaa
    21001 aaggatcata ccagtcctgt ttcagttgag attgactctc cccagcaaca ctatgatgca
    21061 gtgggatcat ggcgcaggat aacatatacc tacgatgatg gctgccagat aatccttgac
    21121 ggcgagaacc gcgataagga tgctgcctat atcgagggtc ctctgggaaa gatattcaag
    21181 ggcttcgtct ctgatatccc caacctggct gaactggtca gggaccttcc cgatcccgca
    21241 ccgcagatca caagcttcac tgattcggtg aagacaagga aaccgtttgc cctgaatgag
    21301 gagaacggtc acagatcatg cacaatagtg aatatgggcg tggtggcgtt gcgcctgggc
    21361 cggaacctca gatttgaccc tgtcaggcag ctcttcattg atgatgaagc ggcaaatgcc
    21421 ctgcttgacc agcctatgcg gggcgggtgg catatgtaaa taatttgaaa aagatatcat
    21481 gaacagaatc aaaaacctat tgatcctgac ggccctggta ctcttcagta cggccgtcag
    21541 tgcccaggat gtcaggactc tggggacgaa agtggcagat ctgctggcaa ggatgccggc
    21601 tgataacctg gaactggcct cccggcttat ggaagacatg tatgccctgg gtgatgaagg
    21661 cagagcgatg atctgtgaac aggtggttcc cgccggcaag ggtgacgacc tgcgtgcccg
    21721 ctatgcaatt gcatcactca cggctcacct gtcaaaggac agtgacgatg ccaggaaacg
    21781 tgcatgggag agtcagtgca tacagttcat gaaaagtgcc ggtgacaggg aggtgaggtc
    21841 tttcttcatg agacagctca gcctgacagg ctctgacatg actgtcagtg cactgtctga
    21901 ttatgtcagt tcccctgaga tgtgtgatga tgccgtcatc gtacttcagt cggttggcag
    21961 cgaggcggca gcttctctcc tttattcatc gctcagcgcc ggcgagtgcc cgtgtgcggc
    22021 tcaggtgatg gtggccctgg ctgagagacg cccgggtacg gctacagaca tatacatgaa
    22081 gagatacctt ggaggcaatg atgcagaaag ggcagctgcc ctctttgcca tcgcttcaac
    22141 aggcgctccg gaggctgctg aaatacttat tgatgcagct acggcagcct cgttccgccg
    22201 tgatccaacc ggtgcggtcc aggccttgct tctctatgca aaaaaaatag gcctgtctgg
    22261 cgaggtaaaa aaaatggaac gtattgcaag ccaggtgatt gatgccagcc gtgaacagga
    22321 ggcagcttcc cagaggcttg ccgccatgag cgttatcgcc gaggtcaagg ggagcgatgc
    22381 cctgggaatg cttcttgagg ctgccgatga tcctgacctg gccatcaggg ggggagtgct
    22441 gcgactggct gcaggcatac ccggaagtga cgtgactaag aaatggatca acaggtacgc
    22501 caaagtatcc gatgctgcga aaccggaaat actgttcatg cttggggaga gaggtgatga
    22561 gctggcagtg ccgctgatga tgaaagcgct tgacgatccc tcgcatgaag tctcggcagg
    22621 cgccgtaacg gcacttgcgc gactccaggg aatcagggca gttgatcctc tcatttcatg
    22681 gattctgaaa tatgacagcg aggagggcca ccgcgctgcc gcaacagccc tcacaaccgt
    22741 actcgacagt acgggcatga gtaagatagc tgcttcactg cccggatcaa aaggcaactc
    22801 aaccgtcacc ctcatcaggc ttcttgcctg gtcaggcgaa agcagctact tcagcaacat
    22861 cattccgttg accggttcgg atgacattgc tatcagggca gcagccatct cagcactgcc
    22921 ggcactggct tcatatgatg accagacgga gataatagcc ctccttgatg caacatcaga
    22981 cagggctgag gtgactgaac ttcagcgggc actgctgagt gctgcaatga ggggtgacag
    23041 ccccggttcc cggtcggcag tcatcctgaa agcgcttgac agcagccgtg acaggctgaa
    23101 gctaattccg ctcctggcag gtaccggagg tgaagaggcg ctgaaaagag tggctgcgga
    23161 gtttgagaac ggcgatgcca tcacccgtga tgtctgtttt gatgccctct cgcactgggc
    23221 tgaccactcg gcatcccggg cactgctcga tattacagcc tcagggaaca aaaccttcgg
    23281 aggaccggca tttgacgcct atatgcggac ggtctcggct gcaccgctga cacctgaacg
    23341 caagcttctg ctgataaagg acatagctcc ctatgccgca gctcctgatg ccagggccgg
    23401 gatggttgcc ttcgctgctt cccttggcct tcggcaggtt gaattcttta tcgctccata
    23461 cctgtctgac cctgtggaag aggtcagaaa tgcggcactg gaggctctgg aatcactcaa
    23521 actgcctgaa gatgaagtat tcgaaccgtt gttcaacggc aaggacctca ccgggtggca
    23581 gggccttgtc ggtaatcctc tgaccagagc caggatgaaa cctgaggaac tggcagcaaa
    23641 acagaaggaa gctaacctga aaatgcttga aaactggagc gtcagggatg gcatgatctg
    23701 gttcagcggc aggggtgaca acctgtgcag catgaaggag tatggtgatt ttgaaatgta
    23761 tgtcgactgg cgtatcacca aagatggtga cagcggaata tacctgcgag gttcaccaca
    23821 ggtgcagata tgggatacct cgcggaccga tgccggtgcc caggtgggat caggtggact
    23881 ttataacaac cagaagaatc agtcagcacc tgttaaagtg gctgacaatc ccgtggggga
    23941 atggaacaac ttccgtatcc tgatgcaggg tgagaatgtc tcagtctggc ttaacggtga
    24001 gctggtggtt gataatgtca tcatggagaa ctactgggac aggtcgatcc ccatcttcag
    24061 gaaggggccc atcgagctcc aggcacacgg aaccgacctg gccttcaggg atatatacgt
    24121 gcgggagata aagccttccg aatataatct cactgacaaa gagaaggagg ccggattcgt
    24181 tgcattgttc aacggtaaga atcttgacgg atggattggc aacaaggtct cctatacggt
    24241 agaagacggc atgatagtaa tcaagccggg caatgacagc ggcggcaacc tgtacaccga
    24301 aaaagagtat tctgatttcg tcttccgctt cgagtttcaa cttactcccg gggccaataa
    24361 cggcctggga atacgcactc cggctgaagg tgacgctgca tatgtgggca tggagttgca
    24421 gatactcgat aatacagcat ccatttatgc cgatcttgaa caataccagt atcatggatc
    24481 cgtatatggc gttatcccgg cacggagggg ttttctgaga ccggtagggg aatggaatta
    24541 ccaggaggtg accgtacagg gtacgaaggt gaaagtggtg ctcaacggaa cggttatcgt
    24601 tgacggggac attgcaggtg ccatcgccaa tggtaccatt gatcacaagg aacatccggg
    24661 actcaggaac agcagcggac atatcgggtt cctggggcat ggctcggtgg taaaattcag
    24721 gaatatccgt atcagggaac tgtaaaatat ttagtcatcc cgtaatggtg gattatcaac
    24781 gatttatcaa cagacatcaa attgagggcg tctgaatttt gaatgcaaca taaaaagaat
    24841 catttttgac cgcccggcac aaggaaggac acattgctgc gttataacaa cgggagcggg
    24901 ggcggagaac tgaataaatt atgagaatac tgatttgtga agataacctg atggtatcaa
    24961 aggcaatgtc tgttgtcata gaaagggagg gatatgatcc ggtgacggta agtgacggaa
    25021 acagggctat tgaagccctg cagaggactg actatgatct ggccatagta gacatacata
    25081 tgccatatca cagtggtctt gaggtgatca ggtacctccg gaccgagctt aagaagcaga
    25141 ctccggtact tattgtctcc gccttcagtg atccgcagat acgtcgtcag gccggcgaga
    25201 tgaaggtaag tggttatctc accaaaccgt ttgatccgga tgatctcctg aaaaaaatca
    25261 gggccgcagt agcagtagtt tagaggatgg ggcacaagaa ttacataaag attttcactg
    25321 tcatggtcat gctgtgcttg acctccggtc tgctccagtc gcagacggtc actgatcctg
    25381 aagaggagta tgcaaggata cggacccttg catttgaagg caaatatccg gaggctgagc
    25441 ccgctgcccg gctccttgtg aagcaatttc ctgaatacgg cgatgcccgt gtcctcctgg
    25501 gccgcataat tgcctggcag ggacggtatg atgaagctgc tgccgtgatt gacacccttc
    25561 tttcctctga accggacaac agcgacgccc tgtcagccct ggctgacata cgcagatggt
    25621 cacatgacag gtcccggcag gttactgccc ctacggatat cagggcaggt tacatgtttg
    25681 acaccttcag tgagccttat gaacgattct ggcaggtttt cacccttgga gccggtcacc
    25741 ggttctcctg gggtactgcc gtggctgcgg ttaactacgg ccacattaat acagggcctc
    25801 catccggcac ttctgacggc gactttcagt ttgctgcaga agcatggcct gaactgacca
    25861 ggaaaaacta tgcatatgtt tcatacgcat acagcaacgg cccatggttc ccacggcaca
    25921 gggccgcact cgagctgtgg cagactctcc cggcaggact ggctgtatcc gcgggggtca
    25981 actattacta ctttgatcac aacatcttta tcgggacagt ttccctggaa aaatatcttg
    26041 caaaatactg gttctcagga agggcatatt tccatttcaa ggatataggg gtcactacct
    26101 cattcttcct taatgccagg agatacctcg gcactgcgga ttatgttcag ctcacccttg
    26161 gtactggcac ggcgcctgat gaaccctatg acatcatcac tgacctggaa cggcagaagg
    26221 catcttccgt caggctgaca tggttcaacc agatcaacca gtggtggtca ttcaggatag
    26281 gtgccgggta ctcgtacgaa aagtacagcg caacatcaaa ccgaaacagg tttgaaggta
    26341 acataggtat aataagaggt atcggacggg caaaatgaag aagagactga ccatagtgat
    26401 tgcctttgcc gttaccgcaa taacattgca gggtttcagc ccggctgaag agaacggaag
    26461 tgactccgtg cgctcacgct ccgctgaatc cggcctccct gtcctttcgt ctttcccgtc
    26521 agaacttacg gttggcggcc tggtctcgct tgccagcggg aactatgcca tccagatggg
    26581 tgctttcagc cgccgttcaa atgccgtggc actaagcgac aagattgccg ggatgacagg
    26641 actcacactt gacatcatcg aagaaggtgg cctgttcaag gtgttcataa acagctcact
    26701 gagaggagag gcgtcatgcc tcttcattcc ggtagcattc cagggagagc cgaaagtgag
    26761 agaacctgca gaggaaataa aacagcctgc agtcaattac ccggtttcac ctgcagaagc
    26821 ctcacctgag ccggatccgg aggccgggat gtctcaggat acagtcagcc agccggccaa
    26881 tgctgaaact gaatcggcac ctgctaccgc agtgataccg gttgaggagt cacagtctga
    26941 accgcaggca accaccacag ctggtacgga atccgccgga gcacagaatg cattacagaa
    27001 actgaggaac ttcttccttc tcaggggaag caatccatgg ttaacccggt ataattactt
    27061 cggaaaatcg gtggcgctgg tgaatgctct gataattacc ataatagcct ctttcgcaac
    27121 aatgctgatc ctcctgctgg tgattctcct caacaggaga aggatggaga aagaggcaaa
    27181 actgaggcag taccttctgg aacagtacca ggcgctcatc attgattacc ttttcggcag
    27241 cagcggtcct gaacccttca gaaagattgc ctccgacaac taccgcaggc aggtgctcat
    27301 tgaccagatg attgacgtga gcgttaacct gaagggcgat tcccgcgaga agctgatgaa
    27361 gctttacatg gatctgaatc ttgacgagga ttcactggaa agagcgcgca gccgccgctg
    27421 gcacaagcgg atcaagggct tccgcgagct tgccttcatg ggtatcaaag aggggaatga
    27481 tgaaatgtac aggtcgctga actccaggaa tgagatactc cggatggagg cccagattgc
    27541 actggtgcgc ctgagcgaaa aggatcactt tgagttcctg tcacacctga agaggccatt
    27601 ctcactctgg gaacagatca cccttcatga tctgatactg cagcatgatc ttccggtacc
    27661 tgacttccag agatggctca gctcggagaa cccgacagtg gtgatgttcg ccctcaggat
    27721 gataagggaa ttcaggcaga tggaagctga gccggagatc agaaaggttc tccttcaccg
    27781 tgaacccagg gtcagcaggc tggcagttga ggtggccggt gatctcggca tgaaaaccac
    27841 gctggagacc atgaaacgca tgtataagtt ccaggagtac aacaactgcc tggagattgt
    27901 caagtcaatg ggcaagatgc ccgagacgtc catgcttgga ttcctcaagc tggtactcga
    27961 caaggaggat gacgttcagc tgcagataga ggctgtgaag gccatcgaaa acatgggtga
    28021 ggttggagtg caggcccttg tcaaggttat gaaatcagag tacaaaaact ataatataat
    28081 agtcaggcac gtgcttgacc gcaggatata ctgataacca tgggattcat tttcgcacat
    28141 ctgatattct ggctgaccct ggtcctcttc gcgacgtacc tcctcctcgg catacactca
    28201 gccctggctc tgaggaagta cctgcgaaag aacagttacg tcaattacaa ctccctggtc
    28261 ctctcccccc tgagccccag gatcaccatc attgccccgg cattcaatga gggcaagacc
    28321 ataattgata acgtccgcac actgctgtca ctttactaca acaacttcga ggtaataatc
    28381 gtcaacgacg gatcaaccga tgataccttc ccgaagatca gggacgccta tgagctggtc
    28441 agggtcaatt attactttga ctaccgcata ccatgcgaaa ggatcagggg tgtctaccgc
    28501 tcaaaggacc cggcctacaa caggctgacg gtcatcgaca agaacaacgg agggaaggcc
    28561 gactcactca atgccggcat caacataagc cgcagtaacc tgatagtgac catcgatgcc
    28621 gactccatca tcgaacccga ttcgatcctg aagctcgtca aacccttcct cgaggagaag
    28681 gagaagaagg tcataggtac aggtggtgta attcgcattg tcaactcatg tgatgtagag
    28741 cgtggtcaca ttcgccagat caacctgccg aagaagttcc tgccgaggct gcaggtgctt
    28801 gactatacgc gtgcattcct cctcggccgt atggcatgga gccatcttga cgggctgatg
    28861 ctgatatcag gagcaatggg catgtttgac cgcgaaacgg tgatcagggc cggcggatac
    28921 aatgtcaaga ccgttgggga agacatggaa ctggtgctca ggatgaggcg ctacatggcc
    28981 gagcacaagc agaattatga ggttacatat atacctgacc cgctatgctg gactgaggta
    29041 cctgctgacc tgagatctat gcgcaaacag aggacaaggt ggacgagagg cctgattgaa
    29101 tctctctggt cacaccggaa gatgatgctg aatgtgaaat acgggagact gggactgctc
    29161 ggttatccct actggctcat gtttgaatgg gcggcacctc tgattgcatt cctcggaatc
    29221 atctattcaa tatatcttgc aattaccgga gcgctgaact ggccgttctt cctcctgctc
    29281 ttcctcttcg tgtacagctt cgccgtaagc cttaccacat gggctgttct ctttgaggag
    29341 ataaccttcc ataagtaccg caggaaacgt gatgtgctcc ggctgatagg cacagccatg
    29401 cttgaaccgt tcttctaccc ggtacatacc tattttgctg tcaggggtaa ccttgagtcg
    29461 cttcgcggca agaagggctg gggcaaggcc gaaagaagcg gattccagaa caagaaaaag
    29521 ccggctactg cctgatttac tgccgtcccg gtccgccagc cggcggatgc gggattgatc
    29581 ctccgacttt gccggagtct caaactgcca actggtactg ccgattgctg aatttgtcag
    29641 ctattccaca taatccggaa tcgtggagag tccttcctca attatatgaa gtgatgcctt
    29701 ttccctctcc acggtctgtg gtaccgtcag ccacttcggc cagctgtacc tgtttgtggc
    29761 gttggtgact acaccattgt gccagactga tattgctatt ccgtcagact gcgcagtgat
    29821 gatctcaaaa ttgtaaccgc cgtcatatga gatggcgcca tcattgaccg tcatctgtgt
    29881 cgggtcatag aaatgaagca tggtccatgg gagcctgatt gttattttcc ggccattcca
    29941 ggacacagct gccctgtctc ctccggtaaa ggaggaagcg tgttcaagcg gcaccttgcc
    30001 aatatcctgg tatgtgagct cgaagccgtc attgatccat ctcattacct tccacggagc
    30061 gccgtctgtg gttgtgcttt taaacttctg caccgggtca gtgagattga agcgtaccgt
    30121 caggccgttc atgtcatagg cctccgtcac atgatgcagc gccgtgtcat catcaagtgc
    30181 gaaagtgaga aggaattcgg ccctgttccc tgtaatggcg ccgtttggca gcgtagattc
    30241 gccggtattg ccgaggtagg tgtcaaaagc caccataaat tcatcggttg gtaacaggtc
    30301 agctgccgtg ttgatctcga cgtacaggaa tccttcgtca tgggttgccc tgacagagct
    30361 caccgggcct gatggctgat cagtactgaa tggcacaaaa ccgggagtct ctttctgttc
    30421 gaaggccagg aggccgaagc actgctccgg tgatgtctcg ttatgccaca actggcgtgt
    30481 gggtatggtc tctcctccgg tgttgagacc gtaagcttcc tggtaaagga ctatccatgt
    30541 acgcttaaac cactcatcca tccacgcaaa catgaaccct ccggcacagc cggcatcgag
    30601 catgttatgc atcatccgca tgttcttctg accctgctgc ttctccgagt aaccgccgtg
    30661 atgcattgag ctgtacgact ggtgtgcgct gccccagctt gaggggactc caaattctgc
    30721 tatcaccagc ggcatgttgc tgtagtgatt cttcatggca gtgaggtacc ccaggtagct
    30781 gttcgcaccc tggccgtcag tgaaacccag gtaagcaggg tcctggctga tgaaattagg
    30841 atagtacgga taggcatggt agctggcaaa gaggccgggt ccaccgctac gggcaatttt
    30901 tgttatgtca aatgaagcca catcctcatc ggtaaagatc tccgtgggat gctccagcgg
    30961 gtcaagcgtg ggccagcttg agaaactgac aggtctgctg actgagtact cattctcctc
    31021 aaagaccaca accctgtcaa gcatttcagt gataaaagct tcagatgccg tgctgccgct
    31081 gatgctgaaa cgggtcccag aataggaggc attgccggga tggaatttat tcgtcgagtc
    31141 tatctcctgt ggagcaactt cgcggcctat gatatatcct gcagtccact gtgagatatc
    31201 agtcctgtaa attccgtatg acttgccata cctgaatgca atatcggcat tgccatgaat
    31261 gcagtcgatc acctctgcaa tttccgcgct gaatgctgac cggcggctga caagatcata
    31321 gcacagcggg tcagtgccat cctcaatctc ctcgagccag atgccctgaa aaagcagcag
    31381 cggcctttca ggattacgct gattgtattc tgcaagtttc tcatagaata ccggcggatg
    31441 aagggtgtaa atccttaccg agttgaatcc tgcctctccc atacggttga tccatctttc
    31501 ataggtcgca gcagagatgg cgtacgcaat ctcaccgggg aaatatccgg gaggcgagga
    31561 accaaggttc acccccttga atatcattgg ctcgtaatcg ttatctatgt accttgcgat
    31621 gtagtttcct gccactgtaa agggcatcgc gttcctgcgc gtcagaacga agttgatgac
    31681 tgtatcccgt acaagggtta tgtcagtaag tctcaccggg tagtagtccg gggctgttac
    31741 ttcggctgta accgggaacc tgagcgcagg tccgggcagg ggagtttcag aaggcagcat
    31801 cacaggttcc agcccgcttc ccgaagagcc ggaagcgttg ccggaagcct ttacgagcct
    31861 gccgctgtat gaccggcccc tgaaggtgat ggcgtagata tagagcccgg cactcatcgg
    31921 gacattattc tgcccgcatc cgtcccatac ggcattgtaa ctgccttcct gaacatcagg
    31981 aaaaaccatt atcctgacct tttcaccggc caggttgtat atggtaagca tgatatcgcc
    32041 cggggtgttg ataataaaag gtatattgac actgttgtcg aacgggttcg gatagggggt
    32101 gcctgccata aactccccgg ctacatcaga gtaagaaccg gttatcttta ccgaataccg
    32161 gccgtcagtt cctgttgata ccctgaactc cctggaattc agaaagaagg atacacgcgc
    32221 attcattacc ggagatccgc cgcgatctga aacagtgccc gtcactgtaa catcctcagt
    32281 aaaagcttgg ggaataaata ccgtcaggca taagatgctg ataaacaaac gtataatatg
    32341 catggccata cttgtctttt tccggtaaca gctgataatg aacaactatc cataccaaat
    32401 gtaacacaaa gcgttgttaa tatcccggga atttttcaac attggcccgt gatttgtcta
    32461 tattggagga aatccataca tttatacttt gctgcggctt cacatttatg gaaaaaataa
    32521 agatcaagga gaccaccaaa accccctcgg taagctttga cccggagcac ggtctgtttg
    32581 agatgcgggg aaagtcaatt cccgagaact caaacggctt ttacgggccc ctcattcagt
    32641 tcgtggaaaa atatgccggc agccctgctg aaaggactgt gctcagtgtc aggttcgaat
    32701 tcttcaatac cagctcttca aacaggatac acgctttgtt caagcgattc gaaaggatct
    32761 tcctggctga cagggacgtg ctgataagat ggtactgcga aaacggcgat gagaatatga
    32821 gagatgccgg aagggatttt aaggcaattc tccaggtacc gtttgagatt gtcgaagtgg
    32881 atgaactttg attttatcaa ctgtcttttg cgaaagggat ttttccctat tttaggctct
    32941 gagaatttta ccggatggca ctgaactata tctggattgc tttttttctg atcgggtttc
    33001 tgtttgccct gggtcagctc atcttcaatg gtgacacctc ggttttcaac aaaattgtcg
    33061 aatcgacatt ttcaagtgca aagaccggtt ttgagatatc ccttggcctg accggtgtgc
    33121 ttaccctgtg gatgggcctg atgagggtgg gagagaaagg cggcgccgtc agcctgcttt
    33181 ccagggttat cgggccgctg ttccggagac tttttccgaa actcccggcc aacagtcctg
    33241 cacacggctc catgatgctc aacgtggctg ccaacatgct gggcctcgac aatgccgcca
    33301 cccccatggg gctgaaggca atgaaggagc tgcaggaaca caatcctgac aaggaagtag
    33361 cctcagatcc catgatcatg tttttggtgc tgaatgcatc agggcttgtc atcattcccg
    33421 ttacaatcat ggtttacagg gcacagatgg gggcagccaa tccggccgac gtattcattc
    33481 ccatcctgat agcaacctat gtcgccaccc ttgccggact gataagtgtt gccattgccc
    33541 agaagataaa tcttttcaac agggtggtgc ttggttacct gggcggactg acagcaatca
    33601 ttgccggcat tgtctggtat ttcagcaccc tgccgcagga gcagatcaat acaatctcaa
    33661 cgtttgcggc gaacttcatc cttctcagca ttatagtctc attcatcgtg atggcgatgg
    33721 tgaaaaagat caatgtttac gagacattca tcgagggagc caaggatgga ttcaagacag
    33781 caatcacaat cataccctac cttgttgcca tactggtggc aataggtgtc ttcagggcat
    33841 cgggagccat ggacctgctt gttgacggca taacggccgt ggtggcctgg atggggattg
    33901 acaccgcctt taccgaggca ttgccgacgg ccttcatgaa accactgagc ggaagcggct
    33961 cccgcgggat gatgattgat gccatgacaa cacatggccc tgactcattc gtcggccggc
    34021 tggcatgctg cctccaggga tcaactgaca ccgtcttcta tgtactggca gtctacttcg
    34081 gctcggtggg aatcaagaat tcaaggtatg cagtcatatg cggactgaca gctgacatag
    34141 tcggtaccat ctcggccatt ttcgtggcat acctcttctt tgcctgacga gtttttttag
    34201 cagattcatc attaaattcc ggcccaagtt gtatttttgc cgcacttatg acgcatcgtg
    34261 acatcagtgc cccggtgtat aaagtcgtat ttacacagct aattttcacc aaaatgggac
    34321 gcgcatttga atatcgcagg gcttcaaagg aagcaagatg ggacaagatg tcgaagcttt
    34381 ttccaaagct tgccaaggca ataacaatgg cggcaagaga aggaggaact gatccggaga
    34441 tgaatgccag gctgcggaca gccattttga acgccaaggc acaaaatatg cccaaggata
    34501 acatcaaggc tgcaattgag cgggcttccg ggaaggacac ggctgattat gccgagatca
    34561 cctatgaagg taaaggcccc cacggggtgc tcatcatggt tgaagctgcc actgacaaca
    34621 atacccgcac catcgccaac atcagggcat acttcagcaa aaacggcggt acagtggttc
    34681 ccaccggctc cctggaattc atgttctcac gcaaggctgt ttttgaattt gacgctccgg
    34741 aaaacattac aagggatgac ctggaacttg agctgatcga ggcaggactg gaatcacttg
    34801 aggagtatga tgacacatgg tatgtttatg gcgactatgc caattttggc actctgtcaa
    34861 aggcattgga agataaggat atagaggtta aaaagggctc gcttgagcgt gtggctctca
    34921 atcccgtcac cctcaccgag gagcagatgg ccgatgttga gaagctcatt gatcgaatcg
    34981 aggacgacga agacgtccag gcggtttata ccaacatagc ataggaattt cgatttgcga
    35041 tttgcgattt gcgatttggg tcc
//
LOCUS       DAOPXU010000021        33114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_1244_length_33114_cov_12.606431, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000021 DAOPXU010000000
VERSION     DAOPXU010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 33114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33114
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1244_length_33114_cov_12.606431"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..906)
                     /locus_tag="PKK03_03510"
     CDS             complement(172..906)
                     /locus_tag="PKK03_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNX83492.1"
                     /translation="MNNKLKTLSLAVVAVIFTGLGLHAQTVPSGRQMDDPTKRDTVRS
                     IAAYFIPATKSKKTPDSQGFIQRWLLLEPIDKPNRSNTVFTDSYIREAFTTVYFPNQF
                     TALPKDGEKVKVGEQELTWHALESKKFNVKLFRFAYGLNKQVYGVLFWAVTVVNSPQE
                     MKNVRMAVGSNSASMWWLNGEEAVILSGDRRMVMDDCVSPRLTLNKGKNIIRGAVING
                     PGMSDFCVRFLDEKGEPIKDITISCE"
     gene            1150..1977
                     /locus_tag="PKK03_03515"
     CDS             1150..1977
                     /locus_tag="PKK03_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX83493.1"
                     /translation="MKVKFYAIAAFILCSDMAAPAQQSFLDGEAVFKNDEVVFHQIDE
                     HTWVGTGNLVYNESLYLVEGTEKAVLIDAGTRIKDLDKIVATITKKPIMLVATHVHND
                     HTGSAVDRFPEIYINPGDKDYIPQVMPRYKGNIKFLTDGEEIDLGGRKLEVVFTPGHT
                     PGSTTFIDKNAAYGFSGDSFGSGNLLLSMDFSTLIATCERMSTIMENYGIKFLFPGHY
                     YGNNAETKQRVDDMITLSKDVISGKVKGEKKSNGMMGLNLVITDYGVNINYSEKSIK"
     gene            1986..3728
                     /locus_tag="PKK03_03520"
     CDS             1986..3728
                     /locus_tag="PKK03_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HNX83494.1"
                     /translation="MKRLFSLVIAALIFAESSAVSQGSYVENIVITKADIERAGTSIP
                     ASAIGEPVGSVRLYVPRWVESTDATPAYAVIEGSILPADPNGWPINFRVLLPASWSRR
                     AIQMGGGGLNGSITVREGKNPQINKGFVLYGSDSGHQGGMGGGGKALASGPSGAGPDD
                     WALNEEAIRNLGYMQMKKTHDAAMVIIERIYNKRPLFNYYVGGSQGGREGLAVAQRYP
                     ADYDGIISDVPIVSFSTLMLAPELIRIKEKSLASWVTPAKTNAIRAEFLRQCDKLDGL
                     SDGIINNYMASRAHFDVTDGIGTVDPWAGLRGPDGKDPNPEDNSVSARLSDEQIKTLE
                     FVYSRLKFPAPLANGVKSFGMWLPTIDPESFGTIAPSRYKGQEGASDNAQVHSSLGVL
                     GVTGFLMQNLSANPLDYTEDKAMIKRRKQISGWLDATNPDLNAFYKHGGKIILTIGTQ
                     DFIASSGAQLDYYQSLTEKMGQKKLDRFARLYVVPQGGHGLSGRSYKVNGDGDTITVK
                     NIPAPSSNDKLDLLISWVEAGTAPARTLVINTEGRIVAKPEGKGYILCSYPNYPKYTG
                     GEPDQASSYVSSSE"
     gene            3845..4387
                     /locus_tag="PKK03_03525"
     CDS             3845..4387
                     /locus_tag="PKK03_03525"
                     /inference="COORDINATES: protein motif:HMM:NF021143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HNX83495.1"
                     /translation="MSSYCHEQTVRNIITGGDAIPLVTGNNEWTALTTPGYCWFNNDE
                     TTYKNPYGALYNWFAVNNGNLCPVGWHVPTHPEWTRLTDFLGGIYVAGAELKEEGLGH
                     WLSPNTGATNRTLFTAIGGGARNFNGAFMQFGVYGNWWTSSLDVPGWAWYRYIAFNDI
                     YVGVNHYNQPAGMSVRCVKN"
     gene            complement(4502..5005)
                     /locus_tag="PKK03_03530"
     CDS             complement(4502..5005)
                     /locus_tag="PKK03_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNX83496.1"
                     /translation="MSRGFVKDGDQEELPMVPKRAYLPEGVTNFVTRTGMEQLLAEKE
                     TLIRERDELNIAHENERRIAVNYVNARLQLLNSRIAEARIVDPKDQPQDGVTFGATVT
                     IKAVATGKLQTFQIVGVDESDISKGKISFISPLAKALLNKKTGERVALQREKEEIKYD
                     IVDISCD"
     gene            complement(5100..6329)
                     /locus_tag="PKK03_03535"
     CDS             complement(5100..6329)
                     /locus_tag="PKK03_03535"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNX83497.1"
                     /translation="MKKSYGKTGLLYAILMVLTISVTTTSESWSQNSAEPANPALDTF
                     FIKLMDKYKFVGVGACLIKDDRIIWRGNYGYANMETGKMINNESIFQLSSLSKTITAF
                     ALLKLYEEGKIDLDEGIDRYLQFKFRNPYFPDKEITFRMLLNHLSGLAEVTSTGQIVP
                     SKVGRPKSSRGDSNMTLEEYITQLLIPGGQYYSEEYFKNTEPGTAYSYSNIGYSLLGY
                     LVQRISGMDFAEFCRENIFNPLGLHDTGWHLRDLDTSRVIFSYSFSPDDIVPVYRKTY
                     HFGEPGYPAGMVRTTMDDFSRFLIMIMNNGRHGDVQLLKPSTIDLMLSPQNIPNMTSR
                     SFRLIDKSLCWLMIETNGSQYYEMNGFSGSIFTDALYSVEKKTGMIYYFSGINMKNMM
                     AVPEIVNTLDKVLEQTN"
     gene            complement(6397..7809)
                     /locus_tag="PKK03_03540"
     CDS             complement(6397..7809)
                     /locus_tag="PKK03_03540"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNX83498.1"
                     /translation="MKKAVHILILGATVTAAVWFLVAGKKSNSSAEDSAAANYMKYCS
                     GCHGAKLEKFAAKQWMDEEGITPVFNSIKFGLEDLGMPAFEKTFTDSEIESLAAYVKK
                     GIPADRSILEAALTTDALIESEVQKFTVDTVVSGLNVPWGMAFLPNGDMLISERSGSL
                     YIFSGGKLSDPVEGVPRVMAIGQGGLLDICLHPDYDRNGWIYFSYSYLIDGKGKPTGN
                     TAIMRARLKDNSLTDQDIIFMGEPATDRNYHFGSKIAFDGKGHIFFGIGDRGQHFDFP
                     QKLDNHNGKIHRLNDDGSIPADNPFVNTPGAMTSVYSYGHRNPQGTCIDPVTGELWVS
                     EHGPRGGDELNLIRPGVNYGWPVISYGINYDGTVLTNLTGKEGMEQPVFYWTPSIGPC
                     GMTFVTGDRYPDWKNNILLGSLPLKHLERVVMKDNAVIHREQLLTGIGRVRNVVMGHD
                     GYIYLAIETPGKILRLMPVK"
     gene            complement(7878..9791)
                     /locus_tag="PKK03_03545"
     CDS             complement(7878..9791)
                     /locus_tag="PKK03_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX83499.1"
                     /translation="MKTIVELLENSAGKYANNPYLLEKKSDHYESISYTQTRDLAYVT
                     AAGLIAMGLQKGDRVALLSESRTDWVLSELGILHAGAICVPLSILLKEGADLKFRIDH
                     SGSRWIIVSALQYEKIKVIRKDLANLEKIILLDQRETYDEDEIFMGRIRNSGGEYLKD
                     NHEKFMERVNSVGPDDYANISYTSGTTADPKAIILSHRNYTANIEQTLTLMDVPEWWT
                     TLLILPWDHSFAHTVGIYIMMATGASLAAVQSGKSYLDSIKNIPANIREIRPVFLLSA
                     PALAKNFRKGIEKSIKEKGPVIEKLFRHALKIAYKYNGIGWDKGKGFRIFLKPLYMLC
                     DLILFKKIRAGFGGRLKFFFGGAALLDIELQKFFSAIGIPMLQGYGLSEAAPVISGNV
                     EKRYKLGSSGYVVKNLDLKICDEDGKELPAGQKGEIVIRGENVMKGYWKNERATREAI
                     RDGWLFTGDMGYLDKDRFLYVLGRFKSLLISDDGEKYSPEGIEETITDRSPFIDQLML
                     FNNQNKFTTALLVPNGEALARWAKNKNIDISGPQGPEAVLNELDSVIGQYRTGGQYDD
                     LFPPRWLPSAVGVLAESFNADNKLLNSLGKMVRAKIVERYHDRIDSLYSAEARNIVNP
                     ENLAAIKKVLGIR"
     gene            9947..10615
                     /locus_tag="PKK03_03550"
     CDS             9947..10615
                     /locus_tag="PKK03_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNX83500.1"
                     /translation="MTEYIVKIQHIERLTHDVKRFRVEKPAGYTFTPGQATGVAINSP
                     ELRKKRRSFTFTGLNQDPFLEFTIKLYPEHDGLTKELDRLVPGDELVIGIPWGAINYK
                     GRGIFIAGGAGITPFLAIFRDLHARNELAGNRLFYANKTRGDIILEEELRGMLGDDFI
                     NILSREEADGYFSGHISGELLKKHADDLSVQFYLCGPPDMMKSMTGMLAGLGIDLKAV
                     TMDL"
     gene            complement(10635..11219)
                     /locus_tag="PKK03_03555"
     CDS             complement(10635..11219)
                     /locus_tag="PKK03_03555"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX83501.1"
                     /translation="MNKGRIVIFSLLTSAVMTLTSCGGKEQVVIHTELGEIHVALDLE
                     NAPVTSANFLRYVDAGLFDSACFYRVVRPDNQPKDSIRIAVIQGGRYEEEETGGFPPI
                     AHETTEMTRIRHLSGAISMARWTPGTATSEFFICVGDQPELDYGGKRNPDGQGFASFG
                     QVTKGMDVVMKIHSIEAPSQYLDKPVLIYSIRRK"
     gene            complement(11221..12522)
                     /locus_tag="PKK03_03560"
     CDS             complement(11221..12522)
                     /locus_tag="PKK03_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HNX83502.1"
                     /translation="MTKNNNTPLSGRFNIGQDWASVVTGFLLILFVVITGYAIATPSF
                     GGKVGWNRDHDFSGMFGASSLWTSVLFTLVLFAVIAIIGILLSGEKIRKYFTGFIAIF
                     LLAILAQFVSSYAGFKNLGLETVLFSLVIGLLIGNLIRLPEWLRSGVQTELYVKIGLV
                     LLGATILFKDILSAGAFGLIQAVIVVTVVWYFAFWLSRKMKVDDEFSTMLASAVSICG
                     VSAAIATAGAINGDKKKLSFIISLVLIIAIPMMIFLPVIARWMGLSEVVTGAWLGGTI
                     DTTGAVVAAGTIAGETGLKYATIVKFSQNVLLGLAAFAISIFWAYRSRGAGEKGQHVP
                     ARIIWDRFPKFVLGFILASLVFSFILSPETAQSSGKVIKSFSTFWFNLAFISIGLETR
                     FADFKHMESKKPFYSFLIAQTFNIFFTLGVSYLLFQLISPK"
     gene            12785..13531
                     /locus_tag="PKK03_03565"
     CDS             12785..13531
                     /locus_tag="PKK03_03565"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HNX83503.1"
                     /translation="MGHSGKVVIVTGAGSGIGRCVAAAFAAVGARVVIAERNNVTGED
                     AVRYIREQGGEVSFIMTDVSVPGQVTAMVRATAEKYGVIDIVINNAGFGIWKSPLDLT
                     VEEWDSVVNTNLRGAFLCAREAARFMKEHGGGSVVNIASTRAFMSEAGSEAYAASKGG
                     VVALTHALAASLAHSHIRVNCISPGWIETGDYSTLREPDHSQHFSGRVGRPEDIARAC
                     LFLTDDDNEFINGENIIIDGGMTRKMIYEP"
     gene            13642..15450
                     /gene="typA"
                     /locus_tag="PKK03_03570"
     CDS             13642..15450
                     /gene="typA"
                     /locus_tag="PKK03_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNX83504.1"
                     /translation="MRNVAVIAHVDHGKTTLVDRILYQVKLFRDNQVMGELILDSNDL
                     ERERGITILSKNVSVRYKDIKINIIDTPGHSDFGGEVERVLNMAEGVLLIVDAFEGPM
                     PQTRFVLQKALELGLKPIVVINKVDKPNCKPEETQESVFDLMHSLNATEDQLDFPTVF
                     GSSKQGWMGPDWAVPATDVSYLLDTIIRYIPAPRTAEGTLQMRISSLDYSSYQGRIAV
                     GKVSRGTLVPGSQVSLIRRDGSVARNTVKEVYLFEGLGKEKTKKPVPAGEICAIFGME
                     DFDIGDTIADSENPEALPWFRVDEPTMSMLFTSNTSPFFGREGTLVTSRQLRERLFRE
                     TEKNLAMRVEETDSADTFMVYGRGILHLSILIETMRREGFELQVGQPRVLIKEISGVK
                     HEPVEVLTVQVPEEFAGKVIDQVTQRKGEIANIEVRNDRAHLDFRIPSRGIIGLRNQV
                     LTVTEGEAVMAHRFDAFEPWKGELAVRRNGSLIAMETGMAVAYAIDKLQDRGRFFIYP
                     GEDVYMGQVIGENTRQDDIVVNVTKTKKLTNMRASGSDDKAILTPPVRFSLEEAMEYI
                     AADEYIEITPKSMRLRKIMLDENDRKRAKMKAQGSE"
     gene            complement(15530..16018)
                     /locus_tag="PKK03_03575"
     CDS             complement(15530..16018)
                     /locus_tag="PKK03_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83505.1"
                     /translation="MKKEILLGAMALLISTGVMAQTTEPVQTQTKTKKQLRIEAKQQA
                     KVQDQTQSGDPIMIKDQKRTRDQKREKIQDQTQSGDPIMTRDRTRQETHGAAVSETAK
                     QAQTQNQGGVGEMVREQARLQGEAQKSMKQAKTATKNKGAAKAVRTNRPATVKGTGAG
                     RR"
     gene            complement(16082..17422)
                     /locus_tag="PKK03_03580"
     CDS             complement(16082..17422)
                     /locus_tag="PKK03_03580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNX83506.1"
                     /translation="MKNRGMMKLQTKLALFNLLSRLVFSALFIGFLPLIIERINVRQT
                     DNELVNKREEVISLISEVGIEPFILSDTAAGFGSYNILKEEYISLERASTDDEWNYID
                     ITQRIIDNETIDYRVLHYSLKIDGESYLLEIGMSLASITYAARNIRNVILVFLGLFIL
                     VTLLSDLAYTARILRPLRKITGRLGETSFPSFDVAEPLVTSTDDFARLDRALREMMKK
                     ISELFRKEKEITVNVSHELMTPVSVLRSKLENMLLNGNPDPATEAGIEESLRMLHRLK
                     ALVNSMLMIARLESHQYLREESVEICELIREVTGELEPIAEDGEVTVTLRCEINYTMK
                     NANRSLLFSMLYNVVNNAVQHTPKGGRIDIACGESSGRFEVSVSDTGEGMSPEQLDRL
                     FTRFSNRPDHAGNGTGIGLAITKSIADFHDIAIRVLSEPGRGTQFFFFFSVNSL"
     gene            complement(17419..18102)
                     /locus_tag="PKK03_03585"
     CDS             complement(17419..18102)
                     /locus_tag="PKK03_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX83507.1"
                     /translation="MNVLIVEDEKSLAGEIALFLKRENFLCDMAHTGSEASEKIAVNL
                     YDFVLLDLGLPDYNGLDIIREAKEINSDVSFIIITARGAVEDRIKGLDLGADDYLPKP
                     FALAELHARIMAVARRKFRISSPDITLGDFVMKTDSRKLMCGQKEVEITRKEFDLLYY
                     LVINRNKVLARHQLYEHIWGNTLDDQYDSNFIDVHIKNIRKKLNAHAPAPWLETVRGV
                     GYRISTDNQ"
     gene            complement(18112..19014)
                     /locus_tag="PKK03_03590"
     CDS             complement(18112..19014)
                     /locus_tag="PKK03_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83508.1"
                     /translation="MRRSQMCTYAIACSLLLQAFAVPAAAQPASGSHTHDTTGTDGKG
                     SIHSLYAGSGFGNNLLYLGTTMSQDHSFGYASASYGLFNKLYISAAGYSVSNFRPFTA
                     FYSLGLSFNHAFNSWFDISTSLSGYNVAASLRDTLFSNFTYADATLGFDWRILYSKIS
                     VGGLFAGESQFYLQTRHSRYFETPSFARNKAFFSFDPYVNLLFGTMVSITTSGGGTET
                     TVSPGYKPWKRKGQGGGNPQPVTTYSESFGPMEIDFGIPVAFSYDFFTLEIEPGYVIP
                     LYSDEGTSGTSGFLLMASLFFRIF"
     gene            complement(19037..19555)
                     /locus_tag="PKK03_03595"
     CDS             complement(19037..19555)
                     /locus_tag="PKK03_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83509.1"
                     /translation="MKITKNILTVLILMAMNSLIINAQEVQIMPDTSAVRQQKARKVQ
                     EKEQERTGEQQGQNDQTGNQNQSQNQSASNSGQGNQNQSQNQSASNSGQGNQNQNQNQ
                     SASQGQAANNSQAKAQGQGSSASGVKKIQGARPDWSKARGARPASVERPSGSRIPKGA
                     GKPGGAKGPGKR"
     gene            19996..21651
                     /locus_tag="PKK03_03600"
     CDS             19996..21651
                     /locus_tag="PKK03_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNX83510.1"
                     /translation="MLLLDWIVLGAFVLALVAFIVISMRGKKDTSTDYFLAGRDATWV
                     AIGASIFASNIGSEHLVGLAGAGASSGMAMAHWEMHGWLILLLGWLFVPFYSRSGVFT
                     MPEFLEKRYNKESRSVLSIISLVSYVLTKVAVTVYAGGVVFKDVFGIESIWGIDFFWI
                     SAIGLVLITGIYTTLGGMKAVLWTSVLQTPVLLAGSIAVLIAGLIKVGGWDELMHICR
                     AVPVNEYGDTMTQLIRSPKDATFPWTGVLLGSAIIGFWYWCTDQYIVQRVLSGRDQTQ
                     ARRGAIFGGALKLTPVFIFLIPGMIAYALNQQGVITLASSDSAFSTLVKELLPMGFKG
                     LVVGGILAALMSSLASLFNSSATLFTIDFYQKYKPKAPEAELVRVGRIATVIVVILGI
                     LWIPVMKGIGKVLYAYLQDVQSILAPGIAAAFLLGILSKKITPAAGLTGLLTGFTIGM
                     FRLIFTVFKGSLNPDGTIYQVFVGTNWLHYEIINFAIVIVTMIIVSYFTPKIDERRII
                     GLTLGSATAEQKAVTRASWNKWDVIASGLIIAVILVFYAYFWN"
     gene            21682..23169
                     /gene="araA"
                     /locus_tag="PKK03_03605"
     CDS             21682..23169
                     /gene="araA"
                     /locus_tag="PKK03_03605"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HNX83511.1"
                     /translation="MKKGNFELWFITGSQHLYGEETLKQVADDSKAIAAALDKSDLIP
                     VKVVFKPVLTTASAITSICMEANNSPACAGLVAWMHTFSPAKMWTAGLKRLNKPMLHF
                     HTQFNRDIPWNEIDMDFMNLNQSAHGDREFGFICSRLRMERKVVVGHWQDKDVLRSIG
                     AWARAAMGALEARSLRVARFGDNMRDVAVTEGNKVSAQLKLDYEVQGYGIGDLADAVN
                     KVTDKQISALVKQYGDEYKAPAKVISTPAVKEAARIEAGMRAFLEKGGFKAFTTNFED
                     LHGLVQLPGLAVQRLMADGYGFGAEGDWKTAALVRLMKVMAEGLRGGTSFMEDYTYHL
                     DPKNMAILGAHMLEVCPSIAAGKPALEVHPLGIGGKDDPARLVFRTNLGEALAASLVE
                     MGDRFRLIVNTMKVIECPPMPKLPVASVLWKPYPDLKTAATAWIYAGGAHHTSFSQAL
                     TAEHMADFADMTGIEYVLIDENCEIGQFRKELRWNDLYYALNKGL"
     gene            23166..23870
                     /locus_tag="PKK03_03610"
     CDS             23166..23870
                     /locus_tag="PKK03_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNX83512.1"
                     /translation="MTHIYSKNPKHYLAVDCIIFGYDIAEKELKLLLIKRSFEPALGQ
                     WSVAGGFVQEEESLDDAACRVLCTLTGLSDLYMEQLYTYGEVNRDPGARVISTAYFAL
                     IGIHELDPEIQKKNGAHWRSISNLPDLIFDHRQMVDMALRELVEKVKVYPVGFELLPE
                     KFTLVQLQNLYEAIYLRPVDKRNFRKKILSMELLDKQDDKERETSKKGAWYYRFNLEK
                     YKELTRNGFFFNLAVS"
     gene            23920..24609
                     /locus_tag="PKK03_03615"
     CDS             23920..24609
                     /locus_tag="PKK03_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HNX83513.1"
                     /translation="MLEKLKEEVLRANLDLVRHGLVIMTWGNVSGIDRESGLMVIKPS
                     GVSYDKMTAADMVVTDLEGNVMEGTLNPSTDAPTHRALYRAFPGAGGIVHTHSTHATA
                     WAQACRPVPCLGTTHADHFYGAVPVTRKLTEKETADDYEYNTGLVIAEALNGIDPMSM
                     PAVLVASHGPFTWGTTPADAVHNAVVLEEVARMAAISATLADPAEIDRHLLDRHYLRK
                     HGKNAYYGQKK"
     gene            24629..26296
                     /gene="araB"
                     /locus_tag="PKK03_03620"
     CDS             24629..26296
                     /gene="araB"
                     /locus_tag="PKK03_03620"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HNX83514.1"
                     /translation="MHNYTIGVDFGTLSGRAVLVDTTTGEEVAVAVHEYSHGVMDEKL
                     PDGTPLGVDWALQHPQDYLDVFRVTVPMVMKMSMVTPDQVVGVGIDFTACTVLPVKAD
                     GTPLCFLDEFRSRPNAWIKLWKHHAAQDEANRLNTIAEQRKEKFLKRYGGKISSEWMI
                     PKIWQTVNEDPGVYDAADRFIEAADWVVWQLTGRETRNTCTAGYKAIWHKHDGYPSNE
                     FFKALHPRMERLVDEKLLRTLMPVGTRAGSITAAAAGLTGLREGTAVAVANVDAHVSI
                     PAVGITSPGKMLAIIGTSTCHMLLGDTEREVPGMCGVVEDGIIEGFFGYEAGQSCVGD
                     HFQWFVENCTPAEYKAEADAAGIDLHTLLTEKAAKLKPGESGLVALDWWNGNRSVLVD
                     VDLTGMIAGMSLITKPEEIYRALIEATAFGTRMIIETFEENGVPVNEFYAAGGIAEKN
                     AFVMQIYADVIKREIRISGSPQAPALGSAMFGAVAAGKADGGFDTIMDAARVMAKVKE
                     QTYRPIGENTAIYDRLFAEYRILHDYFGRGANDVMKRLKAIRKEVKG"
     gene            26296..28263
                     /locus_tag="PKK03_03625"
     CDS             26296..28263
                     /locus_tag="PKK03_03625"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNX83515.1"
                     /translation="MRKTVLIVVSVMLASCGGNTSRQETAQNGWLKTPSLQSVTVNDA
                     FWKPFIERNRTVTIPYAFGKCEETGRIDNFAIAGGLKKGKHTGERYNDSDVFKIIEGA
                     CYSLLETPDPVLRNYVDSLVTLIAAAQEDDGYLYTTRTIDPVNMAPGAGRERWIDERV
                     SHELYNVGHMYEAAVAHYLATGSDSFLNVALKNAELVASEFGWGLREIAPGHQEIEIG
                     LIKLFELTGNNRWLELARFFIDVRGRQEEYTRHPVGSRFEVYNDSVYLQMHLPVLEQT
                     EAVGHAVRGAYMYTAMADISRVTGDNSYLEASEKIWRDVTRGKIYITGGIGSKEYGEA
                     FGEPFELPNMTAYTETCASVANVFWNHRLYSATGNAMYLDALERTLYNGLISGIGQDG
                     CSFFYPNPLESDGRFERAGWFECSCCPGNVARLLPSMKQYIWSETTAGINLNLFIGSS
                     AHLSTTEGETDVTVETAYPWTGNVRVTVTPSTEKSRFTIGVRIPAWTGGEPAEGDLYR
                     YVTPEKGRVGIRVNGKKYKAPISDGFAHIDREWKSGDVVEISLPMEPRFIEARKEVAA
                     DSGRVALGAGPLVYCLEQADNGPVRELTVDTSVKPVFAFEQSLAGGTGTLTFPVTDST
                     GTVKQAKAVPYYSWANRGKGEMTVWIKTTNK"
     gene            28267..30684
                     /locus_tag="PKK03_03630"
     CDS             28267..30684
                     /locus_tag="PKK03_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNX83516.1"
                     /translation="MKINGRLTIFAAAAAITVASCTGNKGDGNYPIRPVPFTSVKMTD
                     NFWAPRIKKNHEVTIPIAFGYCESTGRIKNFEIAGGLDTGAFQTIYPFDDSDVTKIIE
                     GASYSLQTYPDPKLEAYLDTLIYKIGLAQEDDGYLYTNRTIAELGYGKLHEWAEGKRW
                     EKTNILSHELYNLGHMYEAAVAHFQATGKRDLLDISIKSADLVDKDFGWDAFVSYPGH
                     QVIEMGLVKLYRVTGEKRYLDLAKFFLDARGARDDGEEYSQSHKKVVEQTEAVGHSVR
                     ATYMYSGMADVAAILGDEAYVNAITKIWEDLVYRKMYITGGIGASGGNEGFAEPYHLP
                     NMSAYCETCASIGDIFFNHRLFSLHGEGKYFDILEKTLYNGALAGVNLAGDRFFYPNP
                     LESNGQHERSAWFGCACCPSNVCRFIPAIPGYIYSVTNKDLYVNLFASNNADIELGSR
                     SVRIAQETNYPWEGKVTMIIDPEKAGRFTVRVRIPGWARNEAIPGNLYSFAGQSEEKH
                     KLIVNGAKIDAPIENGYAVITRKWQAGDRIELDIPMPVRTVVADEQVKEDEGKYAVQR
                     GPLMFCAEWPDNADGHVLGLVVDENPEFATAFRPDMLNGTEVITTRARQASRNIDGTV
                     SLGDYHDLTLIPYFLWNNRGPGEMKVWLPFKETSARPTPAPTIAYTSKVTGSMQNKAL
                     ASITDQFEPANSNDHTWPYYHWWPNNNRWEWVRFDFAGPETISKVKVYWFDDGPSGGC
                     RIPDEWEVEYLAGGTWRKTSPVSPLTVTKDAWNTAEISPVKASGIRINVKLNQEFSSG
                     IHEVIIE"
     gene            30852..>33114
                     /locus_tag="PKK03_03635"
     CDS             30852..>33114
                     /locus_tag="PKK03_03635"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HNX83517.1"
                     /translation="MKKNLLLIFTAAMFLSSCSGGGVQDTLIPNPSAEEGVGSTVTGW
                     NAETGRGGMIAFYDYEAHHGKRSLMLQSDRPAVGRWTNRVQLKPWSVYKFTGWVKTEN
                     VQAPGRKGAGFRLSVSGDSFTGISGTTDWQKVEITFETGNDDSAVLSCMLGEGPAAGR
                     AWFDDMSLKLVSSDKPNTAVTIDLADRKEPMSEYIYGQFIEHLGRCIYGGIWAEMLQD
                     RKFWYTPGDRESPWQVNGKMDLFSVDRVSPFTGDKTPVLAIDGSGAAVIQQSGLGLKP
                     DLEYTGRVILKATPGITGVKVTLSWGEESRTVTIDKPGNTFSAYPLKFQSGEMTHNAS
                     LTIEPEGNGKVWVGTVSLMPSDNIEGFRSDVIALLKELNAPVYRWPGGNFVSGYNWKD
                     GIGDRDRRPPRKNPAWQGVEHNDVGIHEFMTFCKILGTEPYIAVNAGLGDSRQAREEV
                     EYCNGSSATPMGKLRAANGHAEPWNVKWWSIGNEMYGDWQLGHMSTDDFVRKHNAFAD
                     AMKSVDPDIKLIAVGELGDWDRMVLTNCSESMDLISEHFYRQDWHGGGLMSHVKQIPD
                     AIREKADAHRQYRQEIPQLEGKDIRICMDEWNYWYGPHIYGELGTRYFMRDAMGIAAG
                     LNEYSRQSDMVFMANYAQTVNVIGCIKTNTTSSVFDATGQVLKLYRQHFGTVPVTISG
                     ETRPVDIAATLDSEGRTLTISVINATRDEVKIPVTLLNGTVSGEGELWQVTAPDEMAT
                     NEPGRPENVKITGP"
BASE COUNT         8236 a         7869 c         8728 g         8281 t
ORIGIN      
        1 tcacattcca tgatcgttga tggatcatgt ataaatggtt tcccgatttg tgctgccact
       61 gttccggtta aaataaatga taacagggct gtaatgcccg atattttaat tcttgatttc
      121 ataattctat tatatatata attgagatct taaaatattg actgactgtg tttattcaca
      181 actgattgtg atatctttaa taggctctcc tttttcatcc agaaagcgaa cacagaaatc
      241 actcattccg ggaccattga ttactgctcc cctgattata ttcttccctt tgttaagtgt
      301 cagcctgggt gagacacaat catccattac cattcgcctg tctcccgaaa gtataactgc
      361 ctcctcgcca ttcagccacc acattgatgc cgaatttgaa cctaccgcca ttctgacatt
      421 tttcatttcc tgcggactat tgactacggt taccgcccag aacaggactc cgtaaacctg
      481 tttgtttaac ccataggcga aacgaaatag tttcacattg aacttcttgc tctccagtgc
      541 atgccatgtc aactcctgtt ctccgacttt tactttttct ccgtctttgg gaagagcagt
      601 aaactggttt ggaaaataca cggttgtaaa tgcttctcta atatagctgt ctgtaaatac
      661 agtattactg cggttgggtt tgtctattgg ctctaacagt aaccaacgtt gaataaatcc
      721 ctgagaatcc ggtgtctttt ttgattttgt ggccggaata aagtatgctg cgatgctccg
      781 tacagtatcg cgttttgtgg gatcgtccat ctgacgtccg gatggaacgg tttgcgcatg
      841 taatcctaat cctgtgaaaa ttacagcgac aaccgccaat gacagtgttt taagtttgtt
      901 gttcatcgtt tcttttaaaa tggttacgat tgtgattctg gaaaaaatag tcatttcttg
      961 tgctaaacta cattatattt ttagccacag attacgttga ctgaaaatga aaaggaattg
     1021 attgataact gtaagaaaaa aatccattgg gggttaattg acctgaacga aatggaacag
     1081 gaagtaaaat tgatggctac ttttatccat gtaaattttt aatatccgtt tttaaatatg
     1141 cctgatacta tgaaagtgaa attttatgca attgcagcat tcattctatg ttcagatatg
     1201 gctgcgcctg cacagcagtc ctttctggac ggagaagcag ttttcaaaaa tgatgaagtt
     1261 gttttccatc agattgacga gcatacctgg gtaggaaccg ggaatctggt gtacaacgaa
     1321 agtctgtatc tcgtggaagg aaccgagaaa gctgttctga tcgatgcagg tacaagaatt
     1381 aaggatttgg acaagattgt ggctactatt acaaaaaaac caattatgct ggttgccaca
     1441 catgtgcaca atgatcacac gggctcggca gttgatcgct tccctgagat atacatcaat
     1501 cctggtgata aggattatat tccacaggtt atgcctcgtt ataaagggaa catcaaattc
     1561 ctgactgacg gtgaagagat tgatttaggg ggaaggaaac tggaagtagt ttttacacca
     1621 ggtcataccc ctggctctac cacatttatt gataaaaatg ccgcttatgg tttcagtggt
     1681 gattcatttg gttcaggaaa cctgctttta agcatggatt tctcaactct cattgcaacc
     1741 tgcgagagaa tgagtacaat tatggagaac tatgggatta aatttctgtt tcccggtcac
     1801 tattatggca acaatgctga aacaaagcag cgggttgatg atatgataac tttaagtaaa
     1861 gatgttattt cagggaaagt aaaaggtgag aaaaaatcga atggcatgat gggacttaat
     1921 cttgtcatta ctgactacgg ggttaatatc aactatagtg agaaaagcat aaaataaaag
     1981 aaattatgaa gagactcttt tccctggtca ttgcagcatt gatttttgca gaatcatctg
     2041 ctgtcagtca gggctcttat gtagaaaata tagtaattac caaagccgac attgaaaggg
     2101 cgggtacctc aatcccggct tcagccatcg gagaacctgt cggttcggtc aggctttatg
     2161 taccgcgctg ggttgaatcc accgatgcca ctcctgcata cgctgttatt gagggttcga
     2221 ttttaccggc agatcctaat ggctggccca ttaattttcg cgttcttctt ccggcaagct
     2281 ggtctcgccg tgccatacaa atgggtggcg gtggactgaa tggatcgatc acagtcaggg
     2341 aaggcaaaaa tccgcagatc aacaagggat ttgtgttgta tggcagcgac tcgggccacc
     2401 agggaggaat gggtggtgga ggcaaagccc tggcctcagg tccgtcagga gccgggccag
     2461 atgactgggc actcaatgaa gaggcgatcc gcaatcttgg gtatatgcag atgaagaaga
     2521 ctcacgatgc cgcaatggtc ataattgaaa gaatttataa taagcggccc ctcttcaact
     2581 attatgtcgg aggttctcag ggcgggcgag aaggacttgc agttgcacaa agataccctg
     2641 ctgattatga cggcattatc tctgatgtcc cgatagtcag cttttcaact cttatgcttg
     2701 ctcctgaact gatcagaata aaggaaaaat cattggcaag ctgggtgacg cctgctaaaa
     2761 ccaatgcaat cagggctgag tttctgagac aatgtgacaa acttgacgga ttgtctgacg
     2821 gtataatcaa caactacatg gcatcccggg ctcattttga cgtaacagac ggtatcggga
     2881 ctgttgatcc atgggcaggg ttgcgcgggc cggacggaaa ggatccgaac cctgaagata
     2941 attctgtatc ggccaggctc tctgatgaac agatcaaaac tcttgaattc gtatatagcc
     3001 ggttaaagtt ccctgctcct ctcgcaaatg gagtcaagtc attcggtatg tggcttccga
     3061 ctattgatcc ggaatcgttt ggaacgattg ctccttcccg ttataagggg caggaaggtg
     3121 cgtctgataa cgcccaggta cattcatccc tgggagtcct tggagtaacc ggattcctga
     3181 tgcagaatct gtcggctaat ccattggact acactgagga taaagccatg atcaagcgcc
     3241 ggaaacagat ttccggatgg ctcgacgcaa caaatccgga tctgaatgca ttttacaaac
     3301 acggaggaaa gataattctt acgatcggca cgcaggattt tatcgcctct tcgggtgctc
     3361 agctcgatta ctatcagtct ctgacagaaa aaatggggca gaaaaagctt gacaggttcg
     3421 cccggttata tgttgtcccg cagggaggtc atggattatc aggccgaagt tataaggtta
     3481 acggggatgg agatacaata acagtaaaaa acattccggc accaagctcc aatgataaat
     3541 tggatttgtt aatatcatgg gtcgaagcag gtaccgcacc ggcgaggaca cttgttatca
     3601 acaccgaggg acgtatagtt gcaaagccgg aaggtaaagg atatattctc tgctcatatc
     3661 cgaactatcc gaagtataca ggaggagaac cggatcaggc aagttcgtat gtcagttcat
     3721 cggaataagt caatttgaat gctgacagta cctggtctga taattagatg ttgtcgaccg
     3781 gttgaaactt ttaacttcct gaggctccgt acaatcgtcg ttgcaggatc agttgtctgc
     3841 aatattgtca tcgtattgtc atgaacaaac agtaagaaac attattaccg gcggtgatgc
     3901 aatccctctt gtgaccggca ataatgaatg gacagccctg acgacacccg gatactgctg
     3961 gttcaataac gatgaaacca catataagaa tccttacggt gccctgtata actggtttgc
     4021 agtaaataat ggcaatctct gccctgttgg ctggcatgtg ccgactcatc ccgaatggac
     4081 caggttgact gattttctcg gaggcatata tgttgcagga gctgaactca aggaagaagg
     4141 acttgggcac tggctaagcc ccaacaccgg tgccacaaac cggacactgt ttaccgccat
     4201 tggaggaggc gcacgtaact ttaacggggc ctttatgcaa tttggagtat atggcaattg
     4261 gtggacatcc agtctggatg tccccggttg ggcctggtac cggtatattg cttttaacga
     4321 tatctatgtt ggtgtgaatc attacaatca accggcagga atgtctgtca ggtgcgtgaa
     4381 aaattaaagt acatttgatg aggagggatt cataaagccg gttacaatta cggttgaagg
     4441 cgtaccctcg cgaaaagcca ggtgaagaca gaatgaatca ggcaacgaaa taactgccaa
     4501 ttcagtcgca ggatatatcg acgatatcgt attttatctc ttccttttcg cgctgaagcg
     4561 ccactctttc ccctgttttc ttgttcaaca gcgcctttgc gagcggtgag atgaatgaga
     4621 tttttccttt agagatatct gactcgtcta ctccgactat ctgaaaagtc tgtaattttc
     4681 cggtggctac tgctttgatg gtcactgttg cgccaaaggt aaccccatcc tgtggctggt
     4741 cttttggatc aacaatcctt gcctcggcaa ttctgctgtt gagcagctga agcctggcat
     4801 taacataatt cactgcaatc cgtcgttcat tctcgtgtgc gatgttcagc tcatctcttt
     4861 cgcgtatcag tgtctccttc tctgccagca gctgttccat cccggtacgg gtgacgaagt
     4921 tagtaactcc ctccggcagg tatgctctct ttggcaccat tggaagctct tcctgatcac
     4981 catctttgac aaatcctctg ctcatttccg gtattcgacg cctctaaatt agagatttac
     5041 cgaaagaatc caatggttca gggatgtttt cgggtcaccc cgattgcatt ggttgcttct
     5101 taattggttt gttccaggac tttgtcaaga gtgttgacaa tctccggaac cgccatcatg
     5161 tttttcatgt tgattccgga gaagtaataa atcatgccgg tctttttttc tacggaatac
     5221 agtgcatcgg tgaatatgct tcccgagaag ccgttcattt catagtattg tgacccattg
     5281 gtttcgatca tcagccagca gaggctcttg tcaatcagcc tgaatgaacg cgatgtcatg
     5341 ttcggaatat tctggggact cagcatcaga tcaattgtcg agggtttcag cagctgaaca
     5401 tcgccatgcc tgccattatt cataatcatg atcaggaacc gggaaaaatc atccatggtg
     5461 gttctcacca ttcctgcggg atagcccggc tcaccaaaat ggtaagtttt tcggtaaacg
     5521 gggactatat catccggtga gaagctgtag ctgaatatca ccctgctggt gtcgaggtcg
     5581 cggaggtgcc agccggtgtc atgcaaaccg agtgggttga agatattctc ccggcagaat
     5641 tctgcaaaat ccatccctga gattctctgg acaaggtaac ccagcagcga gtatcctata
     5701 tttgagtagc tgtatgctgt tcccggttcg gtgtttttga aatactcttc agaatagtat
     5761 tggccaccgg ggataagcag ttgtgtgata tattcctcga gggtcatatt tgaatcgccc
     5821 cttgagcttt ttggtctgcc tacctttgac ggtacaatct gcccggttga tgtcacctca
     5881 gccaggcccg agagatgatt cagcagcatc ctgaaggtga tttccttatc agggaagtag
     5941 gggtttctga atttgaactg caggtacctg tcaatgccct catcgagatc tatctttccc
     6001 tcctcgtaaa gcttcagcag ggcaaatgca gttatcgtct tggacaggga agagagctga
     6061 aagatacttt cattatttat cattttcccg gtctccatgt ttgcatatcc gtagttgcct
     6121 ctccagatga tacggtcatc ttttatcagg caggcgccaa ccccgacgaa cttgtattta
     6181 tccataagtt tgataaaaaa ggtgtcgagg gccgggttag ccggttcagc ggaattctgt
     6241 gaccaggatt cggaggtggt ggtaactgaa atcgtcagta ccataagaat cgcataaagg
     6301 aggccggttt taccataact ctttttcatg gcttcaatgc tttgtattct actaaaaaaa
     6361 caaggtttta gcaaaactgc tgcatgaaat gtagccttat ttcaccggca tcagcctgag
     6421 gatttttccc ggagtttcta tggccaggta gatatagccg tcatgaccca tgacaacgtt
     6481 ccttacgcgg ccgattcctg tcaggagttg ctcgcggtgg atcaccgcgt tgtctttcat
     6541 aaccaccctt tcaaggtgct tcagtggcag agaacccagc aggatgttgt ttttccagtc
     6601 cgggtacctg tctccggtca cgaaagtcat gccgcatggg cctatcgatg gtgtccagta
     6661 gaaaaccggt tgttccatgc cctctttccc ggtgaggttt gtaaggacgg tcccgtcata
     6721 gtttatgccg tacgagatga ccggccagcc gtagtttact ccaggcctga tcaggttcag
     6781 ttcatcacct ccgcgtggtc cgtgctctga tacccagagc tcaccggtaa cgggatcaat
     6841 gcatgtgccc tgtggattgc ggtggccgta actgtaaaca gaggtcatgg cgccaggtgt
     6901 attgacaaac ggattatcag ccggtattga cccgtcgtca ttcagcctgt ggatcttgcc
     6961 gttatgattg tcaagcttct gggggaaatc aaaatgctgg ccacggtcgc cgatgccgaa
     7021 gaagatatgc cctttgccgt caaaggcaat tttggatccg aaatgataat tgcggtcagt
     7081 ggccggttca cccatgaaga taatatcctg gtcagtgaga ctgttgtctt tcaggcgtgc
     7141 cctcatgatg gccgtattgc cggttggctt gcccttaccg tcaatcaggt atgaatacga
     7201 aaaatagatc catccgttcc tgtcataatc agggtggagg cagatatcca gcaggccacc
     7261 ctggccgatg gccataaccc gtggaacccc ttccacggga tcagaaagct ttcctcctga
     7321 gaagatatac agcgagccgg agcgctctga gatgagcatg tcaccgttgg ggaggaaggc
     7381 cattccccac ggaacattca gtccgcttac caccgtgtca accgtgaatt tctgaacttc
     7441 tgattctatc agggcgtcag tggtgagtgc agcttcgagg atcgacctgt cagccgggat
     7501 gccttttttg acgtaggcag ccagtgattc aatctcactg tcggtaaagg ttttttcgaa
     7561 agcaggcatt ccaaggtctt caaggccgaa ttttatgctg ttgaataccg gggtgatccc
     7621 ctcttcatcc atccattgct ttgcagcaaa tttctcgagc tttgctccgt ggcacccgga
     7681 gcagtatttc atgtaattcg cagcagcaga atcttccgct gaagaattac tctttttccc
     7741 ggcgaccagg aaccagacgg ctgccgtaac tgtcgcaccc aatatcagta tatgtaccgc
     7801 ttttttcatc tcaggattat tgaattagcc aggaacaatt tacataaaaa aggtgataca
     7861 ttttctggct gccggtttca cctgatccca aggaccttct ttatggcagc caggttttca
     7921 ggatttacaa tatttctggc ctcagctgaa tacagggaat caatcctgtc atggtatctc
     7981 tccactatct ttgctctcac cattttcccg agactgttca ggagtttatt atcggcattg
     8041 aaagattcag caaggacacc cactgccgaa ggcagccatc ttggcgggaa caggtcgtca
     8101 tactggcctc ctgtcctgta ctggcctatg actgaatcca gctcgttcag aactgcttcg
     8161 ggcccctgtg ggccggaaat gtctatgttt ttgtttttcg cccatctggc cagtgcctct
     8221 ccgttaggaa ccaggagggc cgtggtaaac ttgttctggt tattgaagag catcagctga
     8281 tcaatgaaag gtgacctgtc ggttatggtt tcctcaatac cctcagggct gtatttttcc
     8341 ccgtcgtctg atatgagaag gcttttaaat ctcccgagaa catacagaaa cctgtcttta
     8401 tccagatatc ccatatctcc ggtgaaaagc cagccgtcac ggattgcctc ccgggtggct
     8461 ctttcattct tccagtaacc tttcattaca ttttcacccc ggataacaat ctctcccttc
     8521 tggccggccg gaagttcctt cccgtcctca tcacagatct taagatccag gtttttgacc
     8581 acatagccgg atgatcccag tttgtaacgt ttttccacat tcccggaaat aaccggggct
     8641 gcttcggaga gaccgtagcc ctgtagcatt ggaatcccga tggctgagaa gaatttctga
     8701 agctcaatat ccagcagagc ggctccgccg aaaaagaatt tcagccggcc gccgaatccg
     8761 gcccttatct ttttgaaaag gatcaggtca cagagcatgt acaggggctt aaggaaaatc
     8821 cggaatcctt tccccttgtc ccatccaatc ccgttgtatt tatatgcaat cttaagtgca
     8881 tgcctgaaca gtttttcgat cacaggtcct ttctccttga tgcttttttc gattcctttc
     8941 cggaagtttt tggccagggc cggggcgctc agcagaaaaa ccggcctgat ttccctgata
     9001 ttcgccggta tgttttttat tgaatcaaga tacgattttc ctgactggac tgctgccagg
     9061 cttgcgccgg ttgccatcat gatgtaaatc cccacagtat gagcaaatga atgatcccag
     9121 ggaagaatga gaagtgtggt ccaccattca ggaacgtcca tgagggtaag ggtctgttct
     9181 atattggcag tataattcct gtgcgagagg ataattgcct tcggatccgc tgtggttccg
     9241 gaggtgtaac tgatgtttgc atagtcatca ggcccgacgg aattcaccct ctccatgaac
     9301 ttttcatgat tgtccttcag gtattccccg cctgagtttc tgattcttcc catgaatatt
     9361 tcatcctcgt cataggtttc cctctgatca aggagaatga tcttttcaag attggccaga
     9421 tccttccgga ttacctttat cttttcatac tggagggcag agacaataat ccagcgcgaa
     9481 ccggagtggt ctatcctgaa tttcaggtcg gcgccttcct tcaacaagat ggaaagcggt
     9541 acgcatattg cgcctgcatg caggatcccg agctcgctga gcacccagtc agtgcgtgac
     9601 tcggagagga gggcaacgcg gtcacccttt tggagaccca tggcaatcag acctgcagct
     9661 gttacataag ccaggtccct tgtctgcgtg tacgagatag actcataatg gtcacttttc
     9721 ttttccagga ggtaagggtt attcgcatac ttcccggcac tgttttcaag tagctcgacg
     9781 attgttttca tccctgctga tctttagtta attatactgc tctctcaaag ttacgtcccc
     9841 ggatggccaa agtcaattat ttttgtctga agtcaggtat gaggcgcggt aaacctgtca
     9901 aacaaatcac acggttacgt gttagtaggg cagataaaag aatcagatga cagaatatat
     9961 tgttaaaatt cagcatattg aacggttaac tcatgatgtg aaaaggtttc gtgttgagaa
    10021 accggccggc tacactttca ctcccggcca ggcaacgggg gtagccataa attcaccgga
    10081 actgaggaaa aaaaggaggt cattcacttt taccgggctg aatcaggatc ccttccttga
    10141 gtttacaata aagttatacc ctgagcatga cggtttgact aaggagcttg acaggctcgt
    10201 gcccggtgat gaactggtca ttggcattcc atggggtgca atcaactata aggggagggg
    10261 gattttcatt gccggcggtg ccgggataac gccgtttctc gccatattca gggatttgca
    10321 tgcaaggaat gagctggccg ggaaccggct gttttacgca aacaagaccc gtggtgatat
    10381 aattcttgaa gaggaactgc gggggatgct gggggatgac tttatcaata tcctgtcaag
    10441 ggaggaggct gatggttatt tcagcggaca catctcgggg gagttgctta agaaacatgc
    10501 cgatgatctc agcgttcagt tttacctgtg cggaccgccg gatatgatga agtcaatgac
    10561 cgggatgctg gccggactgg gcattgacct gaaggcggtg acaatggatc tatgaatccg
    10621 gtactccggc acggttattt gcgcctgatg ctgtatatca gcaccggttt gtcgaggtac
    10681 tgggaagggg cctcaatgct gtgaatcttc atgactacat ccattccctt cgtcacctgg
    10741 ccgaatgagg caaaaccctg accgtcagga tttcttttac ctccgtaatc gagttcaggc
    10801 tggtcaccca cgcagatgaa gaactccgat gtggccgttc cgggagtcca cctcgccatt
    10861 gaaattgcac cgctgaggtg cctgatcctg gtcatctccg ttgtttcatg ggctatggga
    10921 ggaaacccgc cggtttcttc ctcctcgtat cgcccgccct ggatcactgc tatgcgtatt
    10981 gaatccttcg gctggttgtc aggccgcaca accctgtaaa agcatgcact gtcaaacagg
    11041 ccggcatcaa catacctgag gaaattggcg gaagtgaccg gagcattctc cagatcaaga
    11101 gcaacgtgga tctcgccaag ctcagtatgg ataaccacct gttcctttcc accgcaggag
    11161 gttaatgtca tcacagcgct ggtaagcagc gaaaagatga cgattcttcc tttattcatt
    11221 ttatttagga cttatcagct ggaacagcag gtaagagact cccagggtaa agaatatgtt
    11281 gaaggtctgg gctatcagga agctgtagaa tggcttcttg ctttccatat gtttgaaatc
    11341 cgcgaacctg gtctccagcc cgatgctgat aaacgccagg ttgaaccaga aggtgctgaa
    11401 tgacttgata accttacctg atgactgggc tgtctccggg ctgaggatga acgagaagac
    11461 cagcgaagcc aggataaacc cgaggacaaa cttcgggaaa cggtcccaga tgattcttgc
    11521 cggcacgtgc tgtccttttt ccccggcacc cctgcttctg taagcccaga aaatggagat
    11581 tgcaaatgcg gccaggccca gcagaacgtt ctgtgagaac tttactattg ttgcatactt
    11641 gagtcccgtc tcacctgcta tggttcctgc ggcaacaacg gcaccggtgg tgtctatcgt
    11701 accgcccagc caggcgcctg tgacaacctc ggagagtccc atccatctgg caatgacagg
    11761 caggaaaatc atcatcggga tggcaatgat gagtaccagc gagattatga atgagagttt
    11821 cttcttgtcg ccgttgatgg ctcctgcagt ggcaatggct gccgataccc cgcagattga
    11881 gacggcgctg gcgagcatgg tggagaactc atcgtcaacc ttcattttac gtgacagcca
    11941 gaaggcaaaa taccacacaa ccgtcaccac tatcacagcc tggatcagcc cgaatgctcc
    12001 ggccgagagg atatccttga acaggatggt ggcaccaagc agtacgagcc cgatctttac
    12061 gtaaagttca gtctgtactc ctgaccgaag ccactccggc agcctgataa ggttacctat
    12121 cagaaggcct atgacaaggg agaagagcac tgtctccagt ccgagattct tgaaaccggc
    12181 atatgacgag acaaactgcg ccaggatggc aagcaggaaa atggcaatga aaccggtaaa
    12241 atacttcctg atcttctcgc cgctcagcag gatccctatt attgctataa ccgcaaaaag
    12301 caccagggta aaaagaacgg aggtccagag tgacgaagct ccgaacatgc ctgagaaatc
    12361 atgatcacgg ttccatccga cctttcctcc gaacgacggg gtggcaatag catagccggt
    12421 aatcaccacg aaaaggataa gtaaaaagcc tgtaaccact gatgcccagt cctgccctat
    12481 gttgaacctg ccggagaggg gcgtgttgtt gtttttagtc atgccttgaa cagttttttc
    12541 tcctcctgtt gagggtaggg ttaaacactt attatcgatg aatgcaaata tgaggacaat
    12601 tcaggactga catataagca taaaatggta ttcttcgttt acgggggccc tgatacgccg
    12661 tgcacttata ttgcagaacc cggcaaattt ttaaattcgc atgattgccc cggataacgg
    12721 attcatttcc aggtccggga gcagccggag agtcggggca gtaaaactta taattgtctg
    12781 aaagatggga cattcaggaa aagttgtgat tgttacaggc gcagggagcg gaataggcag
    12841 atgcgtggct gccgcttttg cagccgtagg ggccagggtg gtgattgctg aacgaaataa
    12901 tgtaaccgga gaagatgctg taaggtacat aagggaacag gggggtgaag tgagcttcat
    12961 catgacagat gtaagcgtac ccggacaggt caccgcgatg gtcagggcga ctgctgaaaa
    13021 atacggcgtc attgatattg tgattaacaa tgccggcttc gggatatgga aatcgcccct
    13081 cgacctgacc gttgaggagt gggacagcgt ggtcaacacc aatctccggg gggcgtttct
    13141 gtgcgccagg gaggccgccc gcttcatgaa ggagcatggt ggcggttcgg ttgtcaacat
    13201 tgcctccacg cgtgccttca tgtcagaggc cgggtctgaa gcgtatgccg cctccaaggg
    13261 cggagttgtg gccctgacgc acgccctggc ggcctccctg gcgcacagcc acatcagggt
    13321 caattgcatc agcccgggct ggatagagac gggtgactac agcaccctgc gcgaacctga
    13381 ccacagccag cacttctccg gcagggtcgg aaggcctgag gacatcgccc gggcatgcct
    13441 gttccttaca gacgatgaca atgaattcat caatggtgaa aacatcatca tagacggcgg
    13501 catgacccga aaaatgattt atgaaccctg acccctcagg atgctttatc tcttataact
    13561 aaaaaagtta tttccccggg cagcggacgt gacaattctt tttctacttt tgcagcccaa
    13621 acagggaatc atacggaaga gattagaaat gttgccgtaa tagctcacgt agaccacggc
    13681 aaaaccacac tggtagaccg cattctgtat caggtaaagc tgtttcgtga caaccaggtc
    13741 atgggagagc ttatacttga cagcaatgac cttgagaggg agagagggat tacgattctt
    13801 tcgaagaacg tatcggtcag atacaaggat atcaagataa atatcatcga cacgccgggt
    13861 cactccgatt tcggtggtga ggttgaacgc gtcctgaaca tggctgaggg ggtgctgctg
    13921 attgttgacg cctttgaggg gccgatgccg cagacccgct tcgtgctgca gaaggcgctg
    13981 gaactgggac tgaagccgat agtggtcatc aacaaggttg acaagcccaa ctgcaaaccc
    14041 gaagagacgc aggagagcgt tttcgacctg atgcacagcc ttaatgcaac ggaggatcag
    14101 cttgatttcc cgacagtgtt cggctcgtca aagcagggat ggatggggcc tgactgggcc
    14161 gtcccggcaa ccgatgtcag ctatctgctt gataccatca tcaggtacat accggcgccc
    14221 aggacagctg aagggacact ccagatgcgc atttcatcac tcgattattc atcataccag
    14281 ggcaggatag ctgtcggcaa ggtgagccgc ggcacccttg tgccgggcag ccaggtctcg
    14341 ctgatcaggc gtgacgggtc agttgcacgg aataccgtga aggaggtata tctctttgag
    14401 ggcctcggaa aggagaagac caagaagcct gtgccggcgg gtgagatctg tgcaatcttc
    14461 gggatggagg atttcgatat aggtgatacc attgccgatt ccgagaaccc ggaagcgctt
    14521 ccgtggttca gggttgacga gcccaccatg agcatgctct tcacctccaa tacatcgccg
    14581 tttttcggca gggaaggaac gctcgtcaca tcgcggcaac tgagggagag gctcttccgg
    14641 gagactgaaa agaatctcgc aatgagggtt gaggagacag attctgctga caccttcatg
    14701 gtctacggcc gcggtattct tcatctctcg atacttattg agactatgcg ccgcgaggga
    14761 tttgagctgc aggttggcca gccgagggtg cttatcaagg aaatcagcgg ggtgaaacat
    14821 gaaccggtgg aggtgcttac ggtacaggta ccggaagagt tcgccggtaa ggtaattgac
    14881 caggtaaccc agcgcaaggg cgagatagcc aatatcgagg tgagaaatga cagggcgcac
    14941 cttgatttcc ggatcccctc acggggaatt ataggtctga ggaaccaggt gctgacagta
    15001 accgagggag aggcagttat ggctcaccgc tttgacgctt tcgaaccgtg gaaaggggag
    15061 ttggccgtca ggcgaaatgg ttcgctgatt gctatggaga caggcatggc tgttgcttat
    15121 gccattgaca aattacagga ccgcggcagg tttttcatct accccggcga ggatgtctac
    15181 atgggacagg ttatagggga gaacacccgg caggatgaca tagtggtgaa tgtcacgaag
    15241 accaagaagc tgaccaatat gagggcatca ggctcggatg acaaagcgat tcttactccg
    15301 ccggtgcggt tctcgcttga ggaggccatg gaatatatcg ctgctgacga gtatatcgag
    15361 atcactccca aatcaatgcg gctgcggaag atcatgcttg atgagaatga ccgcaagcgg
    15421 gcgaagatga aggcacaggg atcagaatag aagaagatca tccacacagg taaaataaaa
    15481 aaaccggcag atctctccgc cggctttttt ctcagccttt aattccgggt tacctgcgac
    15541 cagcgccggt cccttttact gttgccggac ggttagtcct gacggctttt gcagcacctt
    15601 tgtttttagt ggctgttttt gcctgtttca ttgatttctg agcttccccc tggagcctgg
    15661 cctgttccct taccatctcg ccaacgcctc cctggttctg tgtctgagcc tgttttgcag
    15721 tctcacttac agcagcacca tgtgtctcct gcctggtccg gtcgcgggtc atgatggggt
    15781 caccactctg tgtctggtcc tgaatctttt cccttttctg gtccctggtc cttttctgat
    15841 ccttaatcat tatcgggtca ccactctgtg tctgatcctg gactttagcc tgctgttttg
    15901 cctcgatcct gagctgtttt ttggttttgg tctgcgtctg taccggttcg gtagtctgag
    15961 ccatgacgcc tgtgcttatt aaaagtgcca ttgcacctaa aagaatttcc tttttcattt
    16021 ttccttctgt tttaagttca ttttcagata acagatcaaa aataaacccg tgaaaatgaa
    16081 gctataatga atttacggag aaaaagaaga aaaattgcgt tcccctgcct ggttctgaca
    16141 gaacccttat tgcaatatca tgaaaatcag caatagattt ggttatggca aggccgatcc
    16201 ctgtgccgtt gccggcgtgg tcaggcctgt tgctgaaacg tgtaaaaagc cggtcaagct
    16261 gttccggtga catcccctct ccggtatcag aaacggagac ctcaaatctg cctgagcttt
    16321 ccccgcaggc gatgtctatc ctgccaccct tcggggtgtg ctggacggcg ttgttgacta
    16381 cattgtacag catggagaag aggagagacc tgttggcgtt cttcatagtg taattgattt
    16441 cacatctgag agtgactgtc acttcgccat cctcggcgat gggttccagt tctccggtga
    16501 cctcccttat cagttcacag atttcaacgc tctcctccct caggtattga tggctctcga
    16561 gccgggcgat cataagcatt gagttgacaa gcgccttcag ccggtgaagc attcggagag
    16621 actcttcaat acctgcttcc gtcgccgggt ccgggtttcc gttcagcagc atattctcaa
    16681 gcttgctgcg caggactgac accggtgtca tcagctcatg cgagacattt accgttatct
    16741 ccttctcttt cctgaacagc tcgctgattt ttttcatcat ctccctgagg gcccggtcaa
    16801 gcctggcaaa atcatcggta gatgtcacca gtggctcagc aacatcaaat gacggaaagg
    16861 atgtctcgcc gagcctgccg gttattttcc taagtggcct gagtatcctt gccgtatagg
    16921 ccaggtcaga gaggagggtg acaagaatga aaaggcccag gaaaaccagt atcacattcc
    16981 ggatgtttct ggcagcatag gttatgctgg ccaggctcat tccgatctca agcagataag
    17041 attccccgtc aatcttcagg gagtagtgaa ggacccggta gtcaatggtc tcattatcaa
    17101 tgattctctg tgtgatgtcg atataattcc actcatcatc cgttgaggcc ctttccaggc
    17161 ttatatactc ctccttcagg atgttatagc ttccgaaccc ggcggcagtg tcggaaagga
    17221 taaagggttc aattcccacc tccgagatga gacttatcac ttcctccctt ttattgacca
    17281 gctcattatc agtctgcctg acattgatgc gctctattat cagcgggagg aatccgataa
    17341 acagggctga gaagaccagt ctggagagca ggttgaaaag cgcaagtttg gtctggagtt
    17401 tcatcatgcc cctgttcttc attggttgtc agtgcttatc ctgtatccca cacccctgac
    17461 tgtctcaagc cagggagcgg gagcatgggc attaagtttc ttgcggatat tctttatatg
    17521 tacgtctatg aaattactgt catactggtc atcgagagtg tttccccata tatgctcgta
    17581 gagctggtga cgggcaagca ccttgttcct gttgattacc aggtagtaga gcaggtcaaa
    17641 ctccttcctg gttatctcca cctccttttg tccgcacatc agcttcctcg aatcggtttt
    17701 cataacgaaa tcgccaaggg tgatgtcagg agaggagata cggaacttcc tgcgtgctac
    17761 agccattatc cgtgcgtgca gttccgccag tgcgaagggt ttcggaagat agtcgtcagc
    17821 cccgaggtca agtcccttga tcctgtcttc aactgcgccc cgtgcggtaa tgataataaa
    17881 agacacatca gaattgatct cctttgcctc cctgatgatg tcaaggccgt tgtagtccgg
    17941 cagaccaagg tcaagcagca caaagtcgta caggttgacg gcaatctttt ccgatgcctc
    18001 gctaccggta tgagccatgt cacaaaggaa attctcccgt ttcaggaaga gggcaatctc
    18061 acctgcaagg ctcttttcgt cttcgacgat aaggacgttc atgatccaaa gttaaaaaat
    18121 cctgaagaag agacttgcca tcaggaggaa accgctggta cctgaagttc cctcatcgga
    18181 atagagggga atgacgtaac caggttcgat ctcaagggtg aagaagtcat agctgaaagc
    18241 gacggggatc ccgaaatcaa tctccatagg accgaatgat tcactgtagg tggtgaccgg
    18301 ttgaggattt ccgcctccct ggccttttct tttccatggt ttgtatcccg gagaaacggt
    18361 ggtttccgtg ccgcctcctg atgtggtgat gcttaccatg gtgccgaaga ggaggttcac
    18421 atacgggtca aacgagaaga atgccttatt ccgggcgaag gaaggagttt caaaatacct
    18481 cgaatgcctt gtctgaagat aaaactggct ttcgccggca aacaggccac cgactgatat
    18541 tttggaatac aggatcctcc agtcgaatcc cagggtcgca tcggcgtagg tgaaattgct
    18601 gaagagagtg tcccgcagtg aagctgccac attataccct gaaaggcttg tgcttatgtc
    18661 gaaccaggag ttgaaggcgt ggttgaaact gagtcccagg ctgtagaaag ctgtgaatgg
    18721 tctgaagttg ctgactgaat aacctgcagc ggaaatgtaa agtttgttga acagtccgta
    18781 tgaggctgag gcatagccga agctgtggtc ctgtgacatt gttgtaccca ggtagagcag
    18841 attgtttccg aagcctgagc ccgcgtacaa tgaatggatg ctgcctttgc catctgtacc
    18901 tgtggtgtca tgtgtgtggg atcctgatgc cggctgtgcc gctgcaggta cggcaaatgc
    18961 ctgaagcaac aggctgcagg caattgcata tgtacacatc tggcttcgtc tcatgacaca
    19021 acgattaccg gtttatttac cttttgccgg gtcctttggc tccgccgggt ttgccggctc
    19081 cctttggtat tcgtgaacct gacggcctct caactgatgc cgggcgggct ccgcgggctt
    19141 tgctccagtc aggccttgcc ccctggatct tcttgacgcc ggaggcactg ctgccctgtc
    19201 cctgtgcttt tgcctggctg ttgtttgctg cctgtccctg gctggcagac tggttctggt
    19261 tctgattctg gttgccctga ccggaattac ttgcagactg gttttgactc tgattctggt
    19321 tgccctgacc ggaattactt gcagactggt tttgactctg attctggttg ccggtctggt
    19381 cattctgccc ctgttgttcc ccggtgcgtt cctgttcttt ctcctgcact ttccgggcct
    19441 tctgctgcct tacggctgag gtgtccggca ttatctgaac ctcctgggcg ttgatgatca
    19501 gggaattcat tgccatcaaa ataagtaccg taagaatatt tttcgtaatt ttcatcatca
    19561 ttcctttctc ccggtttccg ggccgtcagc aacttcggtt tatgtgaggt tcagtttatt
    19621 gaactgatac acagcaaaat tatgaattac aagatgaaga aaggatgaaa cttcaatttt
    19681 ttataaaaaa aatagaagtg tatatcttac atttaaatta ttgtttaact tgcatactct
    19741 gaaagaaggg taagcatccg gggagaaggt ggatttggca ccactgaccg gatctgcata
    19801 agtgtggttc tgctgcaggc gggggagcgt gaagttttac aggacaggga gcgggaagtc
    19861 ttgcaggaca gggagcgtga agttctgcag ggccgggaag ctgagagtaa cagatgcagc
    19921 gggatcaggg aattccgcgc tgatacagaa gtgaatacca ttactaattt ataaataacc
    19981 taacacgaag aaatcatgtt actacttgac tggatcgttc tgggggcatt tgtccttgcc
    20041 ttggttgcct tcatcgttat ctcgatgcgg ggaaagaagg acacatcaac tgactacttt
    20101 cttgccggac gtgacgcaac ctgggttgca atcggtgcct caatctttgc atcaaacatt
    20161 gggtcggagc accttgtagg tctcgccggg gcaggagcct cgtcgggtat ggcgatggca
    20221 cactgggaga tgcatggctg gctcatcctg ctgctagggt ggctttttgt tcctttttat
    20281 tcgcgtagcg gggtattcac catgcccgag tttctggaaa agagatataa caaggaatcc
    20341 aggtcggttt tgtcaattat ttcgcttgtc agttatgtgc tgaccaaggt agccgttacc
    20401 gtatatgcag gcggggttgt tttcaaggat gtatttggca tagaatcaat ttggggaatc
    20461 gatttcttct ggataagtgc aatcggactg gtgttgataa ccggtatcta cacaaccctt
    20521 ggaggaatga aggcggtgct ctggacatca gtccttcaga cgccggtatt gcttgccggt
    20581 tcaattgctg tgcttattgc cggtctgatc aaagtaggcg gctgggatga gcttatgcat
    20641 atatgccgtg cagttccggt taacgaatac ggagacacca tgacccagct tatccgttca
    20701 ccgaaggatg ccacgttccc ctggacaggt gttctgctcg gatcagccat cataggattc
    20761 tggtactggt gtactgacca gtacattgtt cagcgtgtgc tttcaggccg tgaccagaca
    20821 caggcccgcc gcggtgccat cttcggtggt gctctcaagc tgactcctgt cttcatattc
    20881 cttattcctg gaatgatagc ttatgccctt aaccagcagg gtgtcattac gcttgccagt
    20941 tctgattcag ctttctcaac tcttgtcaag gagttactcc cgatgggctt caagggtctg
    21001 gtggtcggag gtatcctggc tgccctgatg agttcactcg catcactgtt caactcctcg
    21061 gcgacgctct tcactattga cttttatcag aagtacaaac ccaaggcacc ggaggcggag
    21121 ctggtaaggg ttggacggat agcaaccgtt atagttgtga ttcttgggat tctgtggata
    21181 ccggtcatga aaggcatagg caaggtcctc tatgcatacc tgcaggatgt tcagtctatc
    21241 cttgctccgg gtattgccgc ggcgttcctg ctcggaatcc tttcgaagaa gataactcct
    21301 gctgccggtc tcacgggctt gctgactggt ttcaccatcg ggatgttccg cctgatattc
    21361 accgttttca aaggatcact caatcctgac ggtaccatct accaggtctt tgtcggaacc
    21421 aactggctgc actatgagat catcaacttt gcgattgtga tcgtcacgat gattattgtc
    21481 agctacttta ccccgaagat cgacgagcgg cgaatcatcg gccttaccct gggttcagct
    21541 accgccgaac agaaggctgt tacccgcgca agctggaaca agtgggatgt cattgcctcc
    21601 ggcctgatca tcgcggtaat attggtattc tatgcctatt tctggaacta acaggatttg
    21661 tttatctgat ctataaataa aatgaaaaaa ggaaattttg aactctggtt catcacgggg
    21721 agccagcatc tttatggtga agagactctg aagcaggtgg ctgatgattc aaaagccatt
    21781 gctgctgcac ttgacaagtc ggatctcatc ccggtaaagg ttgttttcaa accggtactg
    21841 accactgcca gtgcaatcac ttcgatatgc atggaggcca acaattctcc tgcatgtgca
    21901 gggctggtag cctggatgca cacgttctct cctgcaaaga tgtggaccgc cggcctgaag
    21961 aggctgaaca aaccgatgct tcatttccat acacagttta atcgcgatat tccatggaat
    22021 gagattgaca tggatttcat gaacctgaac cagtcggccc acggtgaccg tgagtttgga
    22081 ttcatctgct cgcggctgag gatggagcgt aaggtggttg tagggcactg gcaggataag
    22141 gatgtgcttc gctctatcgg agcatgggca cgcgcagcca tgggcgccct cgaggcacgt
    22201 tcgctcaggg ttgcccgttt tggtgacaac atgagggatg ttgctgttac cgaaggtaat
    22261 aaggtgtcag cacagctaaa gcttgattat gaggtgcagg gatacggtat cggagatctc
    22321 gcggatgcag taaacaaggt gacagacaag cagatcagcg cccttgtgaa gcaatacggc
    22381 gatgagtaca aggctcccgc gaaggtgatt tccacaccgg cagttaaaga ggcagcccgc
    22441 attgaagcag gtatgcgcgc cttccttgag aagggagggt tcaaggcttt caccaccaac
    22501 tttgaggatc tccatggtct cgtccagttg cctggtctgg ctgtacagag actcatggct
    22561 gacggctacg gtttcggggc tgagggtgac tggaagactg cagcactggt tcgcttaatg
    22621 aaagtcatgg ccgagggtct caggggaggc acctcattca tggaagatta cacatatcac
    22681 cttgatccca agaacatggc catcctcggg gcgcatatgc tcgaggtttg tccttcgata
    22741 gctgccggga agccggcact ggaggtccat ccactgggta taggcggaaa ggatgacccg
    22801 gcaagactgg tcttcagaac caacctcggc gaagccctcg ctgcttctct tgttgagatg
    22861 ggtgaccggt tcaggctcat cgtcaatacc atgaaggtta ttgaatgccc gccgatgccg
    22921 aaactccccg tggccagtgt gctgtggaag ccttacccgg atctgaagac agccgccact
    22981 gcatggattt atgcaggagg cgctcaccat accagtttca gccaggccct cacagctgag
    23041 catatggccg attttgctga tatgacagga attgaatatg tgcttattga tgagaattgt
    23101 gaaatagggc agttcagaaa ggaactgaga tggaatgacc tgtattacgc tctcaacaaa
    23161 gggttatgac tcacatttat tcaaaaaacc cgaagcatta cctggctgta gactgtatca
    23221 tcttcgggta tgacattgcc gagaaggagc tcaagcttct cctgataaag agaagctttg
    23281 agcccgcact cgggcagtgg tcggttgcag ggggattcgt gcaggaggaa gaaagtcttg
    23341 atgatgcggc ttgcagagtg ctttgcacgc tgacaggcct gtcagacctg tacatggagc
    23401 aactctacac ctacggcgag gttaaccgtg acccgggtgc cagggttatc tcaacagctt
    23461 atttcgccct catcgggata catgagcttg accctgagat acagaagaaa aacggcgctc
    23521 actggaggtc aatttcaaat ctccctgacc tcatcttcga tcacaggcag atggtagaca
    23581 tggcactgcg tgaactggtg gaaaaggtta aggtttaccc ggtgggattt gaactcctgc
    23641 ccgagaagtt taccctggtt cagttgcaga acctctatga agcaatttac ctcagaccgg
    23701 tagacaaacg gaacttcaga aagaaaattc tctcaatgga actgcttgat aaacaggatg
    23761 ataaggaaag agagacttca aaaaaaggag cctggtacta caggttcaac cttgagaaat
    23821 acaaggagct taccagaaac ggttttttct ttaacctggc ggtgagctga caccatttga
    23881 tgctcgggga gcggatttaa ccataaacac tcattgatca tgctcgaaaa gcttaaggag
    23941 gaagtattaa gggctaatct tgatctggtc agacatggcc tggtcatcat gacctggggt
    24001 aacgtcagcg gaatagaccg tgagtcaggg ctgatggtaa tcaaaccgtc aggggtttct
    24061 tatgataaaa tgacagctgc tgacatggtt gtgacggatc ttgaggggaa tgtcatggaa
    24121 gggaccctga acccttcaac agatgcaccc actcaccggg cgctatacag ggctttcccg
    24181 ggagcaggcg gtatagtgca cacacattca acacatgcaa cagcatgggc ccaggcctgc
    24241 aggccggtac catgccttgg cacaacacac gcggatcatt tttacggtgc tgttcccgtc
    24301 acaagaaagc tgactgaaaa ggagaccgca gatgattatg agtataacac aggcctggtt
    24361 atagccgagg cgctgaatgg gattgatccc atgtcaatgc cggcagtact tgttgcttca
    24421 cacgggccgt ttacctgggg caccacgcct gccgacgcag tgcacaatgc cgttgtcctt
    24481 gaggaggttg cacgcatggc agccatttca gccacgctgg cagatccggc agaaattgac
    24541 aggcacctgc ttgacaggca ttacctgcgt aaacacggga aaaacgcata ttacggtcag
    24601 aaaaaatagt tcatttaaat aaatcatgat gcacaattat actataggag ttgattttgg
    24661 aaccctttca gggagagcgg tactggttga tacgacaaca ggtgaggaag tagccgttgc
    24721 tgttcatgaa tattctcacg gggtcatgga cgagaagctc cctgatggta ctccgcttgg
    24781 agtagactgg gcgctgcagc accctcagga ttacctggat gtcttcaggg ttacagtacc
    24841 catggtaatg aagatgtcaa tggttacccc tgaccaggtg gtgggcgtgg gtatagactt
    24901 caccgcctgc actgtactgc cggtgaaggc agatggtacg ccgctctgtt ttcttgatga
    24961 gttcaggagc aggcccaatg catggatcaa actgtggaag catcacgccg cccaggatga
    25021 ggccaacagg cttaacacaa tagctgaaca gagaaaagaa aagtttctga aacgttacgg
    25081 cgggaagata tcctcagaat ggatgatacc caagatctgg cagaccgtta acgaagaccc
    25141 cggagtatat gatgctgctg accgttttat tgaagcagcc gactgggtgg tatggcagct
    25201 caccgggagg gagacacgca acacctgtac tgcaggatat aaggccatct ggcacaagca
    25261 tgacggctat ccttcaaatg aattcttcaa agcccttcat cccaggatgg agagactggt
    25321 tgatgaaaag cttctgcgaa cccttatgcc tgttggcaca agggcaggat ccataactgc
    25381 tgcagctgcc ggacttaccg ggctcaggga aggcactgcc gttgcggttg ccaatgttga
    25441 tgctcatgta tccattccgg ccgtaggaat cacctcaccg ggaaagatgc ttgccatcat
    25501 cggcacatca acctgccata tgcttcttgg cgatacggaa agagaggtgc ctggcatgtg
    25561 cggtgtggtc gaggacggga tcattgaagg attcttcggc tatgaggccg gacagagctg
    25621 tgtgggtgac cacttccagt ggtttgttga gaactgcaca ccggctgaat acaaggctga
    25681 ggcagacgca gcaggcattg accttcatac cctgcttaca gaaaaggcag cgaagcttaa
    25741 gcccggtgag agcggactgg tggcgcttga ctggtggaac ggcaacaggt cggtgctggt
    25801 tgatgtggac cttacaggca tgatcgccgg catgagtctg attaccaaac cggaagagat
    25861 ttacagggcg cttatcgaag ccactgcctt cggcacgaga atgatcattg agacctttga
    25921 ggaaaacggc gttccggtga acgagtttta cgctgcaggc ggcatagccg agaagaatgc
    25981 gtttgtcatg cagatatatg ccgatgtgat caaaagggag atcagaatct ccgggtcacc
    26041 acaggctccg gcactgggat cggcaatgtt tggtgccgtt gcagcaggca aggcagacgg
    26101 cggctttgat actatcatgg atgctgcccg ggtcatggcc aaggtaaagg aacagacata
    26161 ccggcccatc ggggaaaaca cagcaatcta tgacaggctg ttcgccgaat acaggatcct
    26221 tcatgactac tttggccgcg gagccaatga tgtgatgaaa agactcaagg ccatcaggaa
    26281 agaggtcaaa ggctgatgag aaaaacagta ttgatagtcg tatctgtgat gcttgcatcg
    26341 tgcggtggca atacatcccg gcaggagaca gcgcagaatg gctggctgaa aacgccatcg
    26401 ctgcagtctg ttactgtgaa tgacgctttc tggaagccct tcatcgagag gaaccggacg
    26461 gtgaccatcc cgtacgcctt cggcaagtgt gaggagacag ggcgcattga caacttcgcc
    26521 attgccggtg gactgaagaa ggggaagcat acaggtgaac gttacaatga ttcagacgta
    26581 ttcaagataa ttgaaggcgc ctgttattca ctgctcgaga cgccagatcc cgtgctccgt
    26641 aactatgtgg acagtcttgt gacacttatt gctgcagcac aggaggatga cggttatctc
    26701 tacaccacac gtaccattga ccctgtcaac atggcgcccg gggcaggaag agagagatgg
    26761 attgacgagc gggtaagcca tgagctttac aatgtggggc acatgtatga ggctgccgtt
    26821 gcacactatc ttgctaccgg ttcagacagt tttctaaacg ttgccctgaa aaatgcggag
    26881 cttgtggctt cggagtttgg ctggggcctg cgtgagattg cccccgggca tcaggagata
    26941 gagatagggc tgataaaact ctttgaactg acaggcaata accgctggct tgagctggcc
    27001 cggtttttca ttgatgtcag gggcagacag gaggagtaca ccaggcaccc tgtcggatca
    27061 cgtttcgagg tttacaatga ctcggtatac ctgcagatgc atctgccggt actcgaacag
    27121 accgaagctg tggggcatgc cgtgagaggt gcctatatgt acacggcaat ggctgacatt
    27181 tcgcgcgtca caggagacaa cagttacctt gaagcgtcag aaaagatctg gcgtgacgtt
    27241 acacgcggca agatctatat aaccggagga attggttcca aggaatacgg tgaggccttt
    27301 ggtgagccct tcgagctgcc aaacatgacg gcctacaccg aaacctgcgc ttcagtagcc
    27361 aatgttttct ggaaccaccg gttgtactct gccaccggca atgcaatgta tcttgatgcg
    27421 ctggagagga ctctgtacaa cggactcatt tcgggtatcg gacaggacgg gtgcagcttt
    27481 ttctatccca atcccctcga atctgacgga aggttcgaga gggccggctg gttcgagtgt
    27541 tcctgctgcc ccggaaatgt ggcccgtctc ctcccttcaa tgaaacaata catatggtct
    27601 gagacaacag ccgggatcaa cctgaacctc tttatcggtt catccgcgca tctgtcgact
    27661 acggaaggtg agactgatgt tacggtggag accgcttacc cgtggaccgg gaatgtcagg
    27721 gtaacggtaa caccatcaac ggagaagagc cggtttacca tcggcgttcg catcccggca
    27781 tggaccggag gagaacctgc tgaaggcgat ttgtacaggt acgtcacacc ggagaaagga
    27841 agggtaggaa taagagtgaa cggcaaaaag tacaaggctc cgatttctga cggattcgct
    27901 catattgaca gggaatggaa gtcgggagac gttgttgaaa tctcacttcc gatggagccg
    27961 agattcattg aagccaggaa agaggtggct gctgacagcg gcagggtggc gctgggggca
    28021 gggccgttgg tatattgcct tgagcaggct gacaacggac ctgtcaggga gctgacagtt
    28081 gacacctcgg tgaagccggt gtttgcattc gagcaatcac tggcaggcgg cacaggcacc
    28141 ctgacattcc ctgtaaccga cagcaccggt acggtaaaac aggctaaggc agtgccatac
    28201 tactcctggg caaacagggg aaagggagag atgacagttt ggatcaaaac aaccaacaaa
    28261 taaatcatga aaatcaacgg aagactgaca attttcgccg ctgcagcggc aatcaccgtg
    28321 gcttcatgca caggcaacaa gggtgacggg aattacccca tcaggcctgt acccttcaca
    28381 tcggtgaaga tgactgacaa cttctgggcc ccgcgcatta aaaagaacca cgaggtaacc
    28441 attcccatcg ccttcgggta ttgcgaatca accgggagaa taaagaactt cgagattgcc
    28501 ggcggacttg ataccggtgc gtttcagaca atctatccat ttgacgattc ggacgttacg
    28561 aagattatcg agggagcaag ctactcgctt cagacctatc ctgatccaaa actggaagca
    28621 tatcttgata cattaatata taagattggc ctagctcagg aagatgacgg ttacctctat
    28681 accaaccgca caatagcaga gttgggatac ggaaagctgc atgaatgggc tgagggaaaa
    28741 agatgggaga agaccaatat cctcagtcat gagctgtaca accttggtca catgtacgag
    28801 gcagccgtgg cacacttcca ggctaccggc aagagagatc ttctggacat atccatcaag
    28861 tcagccgacc tggttgacaa ggacttcggg tgggatgcat ttgtaagcta tcccgggcac
    28921 caggtgattg agatggggct tgtcaagctc tacagggtta ccggcgagaa gcggtatctt
    28981 gacctggcga agttttttct cgatgcccgc ggcgcccgtg atgatggcga ggagtattcg
    29041 cagagccaca agaaggtggt tgaacagact gaagcggttg gtcattccgt cagggcaacc
    29101 tatatgtatt caggcatggc tgacgttgct gccattctcg gtgacgaggc atatgtcaat
    29161 gccatcacaa agatctggga agacctggta tacaggaaga tgtacattac cggcggtatt
    29221 ggcgcctctg gcggaaacga aggttttgct gagccttatc accttccgaa catgtctgct
    29281 tactgtgaga catgcgcctc gataggcgac atcttcttca atcacaggct cttttcgctt
    29341 cacggtgaag ggaagtattt tgacattctt gagaagacgc tctataacgg tgcccttgcg
    29401 ggtgttaacc tggcaggaga caggttcttc taccccaatc cgctggagtc gaacggccag
    29461 cacgaaagat ccgcatggtt cggatgtgcc tgctgcccgt caaatgtctg caggttcata
    29521 cctgccattc cgggatatat ctattccgtg acgaacaagg acctgtatgt aaacctgttc
    29581 gcttcaaaca atgctgatat tgaactggga agccgaagcg tgaggatcgc acaggagacc
    29641 aattacccct gggaaggcaa agtcaccatg ataattgacc cggagaaagc aggcaggttc
    29701 accgtgcgtg tccgcatccc cggctgggca aggaatgaag ccattccagg aaacctctac
    29761 agcttcgcag gccaatctga ggaaaagcat aagctgattg taaacggcgc aaaaattgat
    29821 gctccgatag aaaatggcta tgccgtcatc acaagaaagt ggcaggcagg agaccggatc
    29881 gagctcgaca ttcccatgcc tgtgaggaca gttgtggcag atgagcaggt aaaggaggac
    29941 gaaggcaagt atgctgttca gaggggaccg ctgatgttct gcgccgaatg gcctgacaac
    30001 gctgacggcc atgtgctggg actggttgtg gatgagaatc cggaatttgc caccgccttc
    30061 aggcctgaca tgctcaatgg caccgaggta atcaccacca gggccaggca ggcttccagg
    30121 aatattgacg gcacggtcag tctgggtgac tatcatgacc tgaccctgat tccgtatttc
    30181 ctgtggaaca accgaggccc cggtgagatg aaggtctggc tgccctttaa ggagaccagt
    30241 gcaaggccaa ctcccgcgcc cacaatagcc tatacaagca aggtgaccgg aagcatgcag
    30301 aacaaggcgc tggcttccat aacagatcag tttgagcctg caaattcaaa tgaccatacc
    30361 tggccttatt atcactggtg gcccaacaac aacaggtggg aatgggtcag gtttgacttc
    30421 gccggaccgg aaaccatatc aaaagtaaag gtctactggt tcgatgacgg tccgtccggc
    30481 ggctgcagga ttcccgacga atgggaggta gaatatcttg caggcggcac atggaggaaa
    30541 acttctcccg tgagccccct gacagtgaca aaggatgcct ggaatactgc agaaatcagc
    30601 ccggttaaag catccggcat aaggataaac gtgaaactca accaggagtt ttcatccggg
    30661 attcatgaag tgatcatcga ataaagagct tgttacaccg gggcttgcct ggagaaaccg
    30721 aaaagagatt gagtaaatga gtggagaatg taattgcatt gttttgtgat tctggattag
    30781 gagtaaaagg ccgcttttta ccggcctttt acgtttataa taaagtgatt ataacaaatt
    30841 ttagcactct catgaaaaaa aacctgttgt tgatttttac tgctgccatg ttcctctcct
    30901 cctgctccgg aggaggggtt caggacacac tgattcccaa cccgtcggct gaagagggag
    30961 taggttcgac tgtgaccggt tggaacgccg agaccggcag agggggcatg attgcctttt
    31021 atgactatga agcccaccat ggaaagcgga gcctgatgtt gcagagcgac agacctgccg
    31081 ttggaaggtg gacgaaccgc gttcagctta aaccatggtc agtttacaaa ttcaccggat
    31141 gggtgaagac tgaaaacgtc caggctcccg ggaggaaggg agcgggattc cgtcttagtg
    31201 tctccggtga cagtttcacc ggaatttccg gcaccactga ctggcaaaag gttgaaataa
    31261 ctttcgaaac cggcaacgat gactcagccg ttctgtcctg catgctcggt gaaggcccgg
    31321 cagcaggaag ggcctggttt gatgatatgt ctctgaaact ggtttcgtcc gataaaccga
    31381 acacagcggt cacaatagac ctcgctgaca ggaaggagcc gatgtcagag tatatctacg
    31441 gtcagttcat agaacacctc ggcaggtgca tctacggagg catctgggct gagatgctcc
    31501 aggaccgcaa attctggtac actcccggcg acagagaatc accatggcag gttaatggca
    31561 aaatggatct cttctccgtt gacagggtca gtcccttcac aggtgacaag acgccggtgc
    31621 tggcaattga cggctccggc gcagcagtga tacagcagtc aggtctcgga cttaaacctg
    31681 acctggaata caccggccgg gttatactta aggcaactcc ggggataacc ggagtgaagg
    31741 tcaccctaag ctggggtgag gagtcacgga ctgtcacgat tgataagccg ggcaacacct
    31801 tctctgccta tccgctgaaa ttccagtccg gcgaaatgac tcacaatgct tccctgacca
    31861 tcgagccgga aggcaacgga aaagtctggg taggaactgt cagcctgatg ccctcagaca
    31921 acattgaggg attccgttca gacgtcattg cattgctgaa ggagctcaac gcaccggttt
    31981 atcgctggcc cggaggcaat tttgtcagcg gctataactg gaaggatggc attggtgacc
    32041 gcgacaggag accgccgcgc aagaacccgg catggcaggg cgttgaacac aacgatgtgg
    32101 gcatacatga gttcatgacc ttctgcaaaa tactcgggac tgagccttac attgccgtca
    32161 acgccgggct gggtgattca aggcaggctc gcgaggaggt ggagtactgc aacggatcgt
    32221 ctgcgactcc aatgggcaaa ctaagggctg ccaacggtca tgccgaaccc tggaatgtaa
    32281 agtggtggtc tatcggcaat gagatgtatg gcgactggca actgggtcac atgtccaccg
    32341 atgatttcgt caggaagcac aacgccttcg cggacgcgat gaaaagtgtt gatccggata
    32401 tcaaactcat tgccgttggc gagctgggag attgggaccg gatggtgctt acaaactgct
    32461 cggaaagcat ggacctgatc agtgagcact tttaccgtca ggactggcac gggggaggac
    32521 tcatgagcca tgtgaagcag atccccgatg caatcaggga gaaggctgac gcgcaccggc
    32581 aataccgcca ggagatacca cagcttgagg gcaaggatat ccggatatgc atggatgaat
    32641 ggaactactg gtacggcccg catatctacg gggagctcgg cacacgctat ttcatgcgtg
    32701 atgcgatggg tatagctgcc ggccttaacg aatactcgag gcagagtgat atggtgttta
    32761 tggccaatta tgcacagact gtcaacgtca tcgggtgcat caagaccaac accaccagct
    32821 ctgtctttga tgcaacaggt caggtgctta aactctacag gcagcatttc ggtactgtgc
    32881 ctgttacgat cagcggtgaa acgaggcctg ttgatattgc ggccacgctt gacagcgaag
    32941 gccggacact gacaatctct gttataaatg ccacccggga cgaagtgaag atacccgtca
    33001 ccctgctgaa cggaactgtg tccggtgagg gtgagctctg gcaggtcacc gcgccagatg
    33061 aaatggccac caacgaaccc ggaaggcctg aaaatgtgaa gataacaggt ccgg
//
LOCUS       DAOPXU010000022        32697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_1270_length_32697_cov_12.994241, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000022 DAOPXU010000000
VERSION     DAOPXU010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 32697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32697
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1270_length_32697_cov_12.994241"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PKK03_03640"
     CDS             complement(<1..880)
                     /locus_tag="PKK03_03640"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX83518.1"
                     /translation="MKRLAFLTILFLPALPLMPQRTVTLWQCYDSATVISPLSGEKEL
                     YAGMTQLRDHNLAAAWFPSLDLGGSFNYQSDVVDISEMLGSMPVPPGSLPSIPHEQYR
                     ATVDLNQVIWDGGATRSAREVERVVSELNMQQSEADIYRLREQVNNLYFSLLLTRSQA
                     EVTSVLISELGTRISEAESGVENGVVPPVTLDVLKAEMIKAEQAAAELGRRHDALALA
                     LEQLTGMSGLKYAGLALPDNLITGYEKVENPDLQLFDTRRLQLELSKNLLKSQRMPRL
                     FGFAQAGYGNPPGNNFL"
     gene            complement(877..1512)
                     /locus_tag="PKK03_03645"
     CDS             complement(877..1512)
                     /locus_tag="PKK03_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX83519.1"
                     /translation="MAKAVVTDSQTRLKIFRAASDIFEEKGYEGARMQEIADRAGINK
                     ALLHYYFRSKDQLFMAVFQVLLKKMFEKIMTIFAADTTFKEKIRQFLDQHIDFLSKNP
                     KLPIFLLNEVSRNPSIAAGLKETILYGEMRDLIYEKHAKELKGYGIKKSDMPQLMITV
                     VSMSVFPYAARDMIALMMPQMHDNRRFKTFMDERKEFAADFILNSLKHRKK"
     gene            1648..2391
                     /locus_tag="PKK03_03650"
     CDS             1648..2391
                     /locus_tag="PKK03_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX83520.1"
                     /translation="MKNFFKKLGRWLLGIVLFLVALMIVFIAMSASSKKKMTPADTGL
                     LTEDVYAVRDKYVNMYLVRDGNDYIAIDAGIKPGNIRGELKKLDIDPDRVRAVFLTHS
                     DADHAGGISMFKRATIYMHRNEEKMINGETGRMLWIGNNIDAKEYTLLDDHTVRIGGL
                     RIKPIPTPGHTAGLTCYLVNDIYLFTGDAVSLRDGVIGLFPKYINKYPRKARRSVQNI
                     LNLEGVMYIFTGHHGFSANYSSAVRGYRE"
     gene            2531..3409
                     /locus_tag="PKK03_03655"
     CDS             2531..3409
                     /locus_tag="PKK03_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNX83521.1"
                     /translation="MKTRTAEKIIPPPPVHFVGDGFRVHNFIPGVYPLTMARMDPFIL
                     LDYNSKFYFSPTAKPRGVGVHPHRGFETVSIAYKGRIAHHDSFGNSGVIGEGDVQWMT
                     ASSGLLHKEYHEKEFARAGGDFHMVQLWVNLPAKDKMSPPKYQPVTNDQMGKYILHGD
                     QGIIEVIAGSYRETKGPASTFTPMALMNARLNAGGKADFEFPSHYNTAALIIEGRVLV
                     NGSVKAPADNFVLFANDGEQFTIEAEEDSIVLILSGEPIMEPIAAHGPFVMNTRTEIY
                     QAFEDVENGKFGYLEE"
     gene            3467..3844
                     /gene="crcB"
                     /locus_tag="PKK03_03660"
     CDS             3467..3844
                     /gene="crcB"
                     /locus_tag="PKK03_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNX83522.1"
                     /translation="MLKNLLIIGSGGFIGSIARYLVSHLNLTVTFHSIPVGTLLVNVT
                     GSFLIGILTGIAEKSMIPGPEWRLFLMVGLCGGFTTFSTFTGENLMLMHNGQLLTVLL
                     YTGLSIFLGFLAVYLGYVTTNLL"
     gene            3847..4209
                     /locus_tag="PKK03_03665"
     CDS             3847..4209
                     /locus_tag="PKK03_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HNX83523.1"
                     /translation="MEKKPNSVLKVFASSTDRLGSQLLYEAVVLRAREAGISGATVYR
                     GMMGYGLSSHIHSSKFWELTEKLPVVVELIDTEEKLRTFYEVIAPELESLPKGCLVTI
                     SPVEVILHKSGEKQGRSR"
     gene            4452..5990
                     /locus_tag="PKK03_03670"
     CDS             4452..5990
                     /locus_tag="PKK03_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNX83524.1"
                     /translation="MLVKTFSAAVSGIEAAPVTIEVDVTRGVRYMLVGLPDTAVKESQ
                     QRIESALRAVGLRWPGRQVIINMAPADIRKEGSSYDLPLAVGILVASEMVSGNCLDGM
                     LLTGELSLDGVVRPVRGVLPMALRAREEGFRCMMVPALNAPEAAVVEGLDIYGVNTLG
                     EVLGYLRGDTTPAPVTVNLRELFSREGNVYDTDFADVKGQENVKRALEVAVAGNHNAI
                     MIGPPGSGKTMLARRLPGILPPLTLEEALETTRIHSVAGKIDNHIPLMTCRPFRSPHH
                     TSSDIALVGGGPVPQPGEISLAHNGILFLDELPEFKRSVLEVLRQPLEDRVITIARAR
                     LSCNFPASVMLIASMNPCPCGFHNHPVRECTCAPGMVQRYMSRISGPLLDRIDIHIEV
                     VPADYQNLSVTAKTENSSVIRERVVKAREIQSERFAGNDGIHSNAQMTPALIRRHCHI
                     DDAGGRLLRAAMEKRGLSARCYDRILKVARTIADLDHSDTIRPDHLSEAINYRNLDRE
                     GWAG"
     gene            6075..7211
                     /locus_tag="PKK03_03675"
     CDS             6075..7211
                     /locus_tag="PKK03_03675"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HNX83525.1"
                     /translation="MELINKLRQKLSDEHLMFAYRGEVTGDNSVSLLTLLEREMEFSD
                     FSMLGRKRLFMFVLENLQNITRHGITRSEDITSLVVYSKTPDGYTVSTGNALKKSEVR
                     GLRSNLHKINSLDPDKIREVYRTMLQDTKIGKKGGAGLGLMEMARKTGNRLDYDFYPI
                     DEKYSYFILSKTIDSGGKGMTEPAQDQSAFDSKRIVQLERMMKSNNIFFIWAGPMTHA
                     ISKELLNFSETTMREAELEQNLHKRVFATLIELLQNVAQHSPGFEAEKHYGIPLAMIR
                     KTSASFIITSGNLIRRSDTDHLTQKLDMVNRYDEEGLKKLLKVALMGQDMSKISTGYM
                     GLLEMARRSGNRLTYKFDDVNDEYAYYTITVRVRAKGFKSPLAG"
     gene            complement(7265..8011)
                     /locus_tag="PKK03_03680"
     CDS             complement(7265..8011)
                     /locus_tag="PKK03_03680"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNX83526.1"
                     /translation="MEGRKTYFASDFHLGLAAGTDPHEREQRVIAWLDRVSADAESVW
                     LLGDVFDFWWEYRKVIPKGFTRFLGRLASLTDSGIGVHIFTGNHDMWMKGYLAEECRV
                     TVHYEPHITTIGGELFYMAHGEGLGSRRIGGRLLFGLFRSRIARVLFSALHPRWGVAI
                     GHAWSKSSRLAKHVSLPFMGEEKEDLVRHTRSVAAGGCRAKYYIYGHRHLPLTYDENG
                     RRMIILGDWFSCRESYAVYDGKELTLVTLQ"
     gene            complement(8019..9173)
                     /locus_tag="PKK03_03685"
     CDS             complement(8019..9173)
                     /locus_tag="PKK03_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNX83527.1"
                     /translation="MKRFLPVLLAALLLAGCNNDNKVIMKGAFTVPAEGMVYLDQSEV
                     DRSTRVDSTEIKRGRFKFVTEVTGPEFYQLRFPGNEFVALLVMPGENISLELGKTPVA
                     MNYTVQGSPGSADIRLLDQQLLVTKTRLDSVRNIYNALSIDDLAVRGPELQKTYTEAV
                     EAQRKFNIKFVVEHISSMSSIQALYQRLDENTYVLYQPRDLQYLKIVSDSLSVRYPVS
                     RHVRALKENVTSELNQMYIDRISDLAMQLPETKSSPELPDINDKMVGVSSLKGKYVLV
                     SFWSTTSEASMNQLPLLRSIYNDYHRKGLEIYQVSLDTDPERWKSVVRFEEIPWISVR
                     EPDPANPIYARLMNITSLPSNLLYDPQGNIVNTNLTGRNLLIRMDQLFNQ"
     gene            complement(9221..10393)
                     /locus_tag="PKK03_03690"
     CDS             complement(9221..10393)
                     /locus_tag="PKK03_03690"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNX83528.1"
                     /translation="MGKKVILMLFCASVLISGCKRGSFSISGTIDGAGDGEYLLLKEV
                     KPGILEPVDSVVPGKDGRFSFRSETGWPAFFMLSMDDDDFLTMLIAPGEKIEVKAARN
                     SMARPSSVTGSEGTSVILGFRKDQDEVVAGLKKLTDTYNDSIDSPRRPLLMDSLDRRA
                     ADIVAGFRARALTLLNENRSSMIPVYLLNQHVVPGLELFEPAKNPELFYSVDSTLYAL
                     YPESDLVLDLHSFVAGLRKSVSVGRQAGEPPVPGELLPDIVLPDPDGDTLSLSSLRGR
                     VVLVDFWAAWCPPCREENPNLVRLYDMYHWKGFDIFQVSLDLRKEDWLEAIRNDRLGR
                     WKHVSDLRYRDSEVVKQFGLTEIPASFLIDREGRVTAVNLRGDDLQKKLEELLAAQ"
     gene            10658..12610
                     /locus_tag="PKK03_03695"
     CDS             10658..12610
                     /locus_tag="PKK03_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNX83529.1"
                     /translation="MGYIKFDKAQVVNLEYSLTREILRSNRAGSYSSTTIVGCNTRKY
                     HGWLVCPVDAMGGERHVLLSSIDSTVVSNGQSFNTGIHKYHGDHYSPKGHKYVEDFDI
                     QDIPGMTYRVGNVIMKQERILVHYEEQLLVRYTIIDTDEPVILQLRPFLTFRSIHQLT
                     HANMWASTRTEPVPHGVKIRMYEGFPYLHMQLSCKAEFVPVPDWYLGVEYVEEQKRGY
                     DYSEDLFVPGFFEMTASKGDVIVFSISTREEKHAGLKAKFTRTVATKIPRSNFGNCLR
                     NAAQQFIEKRGNDTNIIAGYHWFGSWGRDTFISLPGLALARKSEDLYAAVIDTQVRRM
                     KGGLFPNMGDNDNPAFNSVDAPLWFFQALWNYGLDPKETWKRYGAAMKDVLNAYRAGT
                     SFGIHMRENGLIYSGAPGKALTWMDAVVNGVPVTPREGYAVEINALWYNAVCYSLDCA
                     RGARDRAFVKEWEKLPDLISRSFIETFWDEEHGYLADYVNDREMRNMQIRPNMVIATS
                     LPYAMLTRDQMKSILDIVNRILVTPRGLRTLSPSDEGYKGIYCGDQATRDNAYHQGTV
                     WPWLLGPFCDGWLRVYGEGGVSRVRKLITGFEETLTEAGISTISEIYDGDPPHSPRGA
                     ISQAWSVGEILRIFTLLEKNYPENNK"
     gene            12612..13907
                     /locus_tag="PKK03_03700"
     CDS             12612..13907
                     /locus_tag="PKK03_03700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX83530.1"
                     /translation="MRVLMFGWEFPPHIAGGLGTASFGLTRGLAAHRDIEVIFVVPKA
                     YGDEDQSIVRLIGANTVPVAFKKVKYRYFQRPVEKIEVFSKLVPYTDPEEFWKITRGE
                     VADTDVLVRTNEHGKVDFSGTYGPSLMDEIHKYSVVAQVIASEQEFDIIHAHDWLAYP
                     AGIAAKAVSGKPLVIHVHATDFDRSGGNVNPVVYGMEKEGMDAADKIITVSNLTRDIV
                     INKYNVDPEKVETVYNAVEPLHHSELDVPERAYDDKIVTFLGRITMQKGPEYFIEAAR
                     MVLSRMPNVRFVMAGSGDMMERMMRHAAALRITDRFHFTGFLRGNDVFTMLRMSDVYV
                     MPSVSEPFGISPLEAMQSNVPVIISKQSGVAEVLTHAVKVDFWDIEAMADAIYGILTY
                     PALSRMFIMNGKEEVDKLKWEKAAGHVKGIYSEVLQQTR"
     gene            13913..15355
                     /locus_tag="PKK03_03705"
     CDS             13913..15355
                     /locus_tag="PKK03_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HNX83531.1"
                     /translation="MSTSQKKAICLYFQVHQPFRLRRYRFFEMGHEHYYYDDFMNESI
                     LRKIATNCYLPANRIILDLVKKHKGKFRVTFSLTGIVIDQFRLYAPEVLASFRELAET
                     GCVEFLAETNSHSLASLMSRERYELQVRVHREKMMEYIGYEPTSIRNTELIYSDEIGS
                     WMAELGYKAVVTEGAKHILGWKSPNYLYCNAVNPRLKVLLRNYTLSDDIAFRFSSKEW
                     SSYPLTAEKYAGWIKGLASTSELINVFMDYETFGEHQKKETGIFDFLKHLPETIIKKT
                     PFNFMTVSEVADNYQPVSLLHVPSPISWADEERDLTAWMGNELQTAAVNKLYSLSDRV
                     GRCNNEQLMRDWVYLQSSDHFYYMSTKFFSDGAVHAYFNPYENPYQAFMNYMNVLSDF
                     EIRLNTVCPLNIHDEEVNRLSADIKKRDHMITELQEKLARLEKKPVVRTKPGAAASKK
                     TAPKTAAKSKTVAAKAVTKKVAARKKSGRK"
     gene            15428..19651
                     /gene="glgP"
                     /locus_tag="PKK03_03710"
     CDS             15428..19651
                     /gene="glgP"
                     /locus_tag="PKK03_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNX83532.1"
                     /translation="MKADFLFETSWEVCNKVGGIYTVISTKALNVTYEFGDNYILIGP
                     DVWRDDKSNPEFTPDDTMFQSWKTVAAQEGLIVKTGRWNIKGNPRVMLVDFTPYFGQQ
                     NEIFARFWETYKLDSISGQWDYIEPALFGYAAGKVIESFTNFYREYPRVIAQFHEWMT
                     GMGVLYLEKHAPWVATAFTTHATVLGRSIAGNNRQLYSRLKEFNPVQTAHEFNVTSKQ
                     SLEKISAQEADVFTSVSEITGRECEAFLGKKTDVITPNGFEDTFVPAPENFDEARRAA
                     RDRLLSVAQALLGYNLPADTLLVSTSGRYEYRNKGIDLFLSALGEVNRKTDSSRQCVA
                     FILVPAYHKGPRQDLAAALANGTIVTEGDKYLTHGLHYVNDDPVMNQLIREGIHNIAS
                     EKVKVIFVPSYLNGNDGIFNMEYYDLLIGFDLTLYPSYYEPWGYTPLESLMFHVPTVT
                     TNLAGFGMWVDTYFKEPGNGCLVLNRTDNNEKEVITGMEEFIRKFMHMNPEEAAAASQ
                     KAWEISRIANWNTLFSYYSEAYRFALEKSEERRDQPRDYTRILEATEVQMRKPFQAPV
                     WKDIYVQSELPERIAYLKELSSNLWWSWNSEAEFLFRRMDPTLWEEVGHNPKRLLEAI
                     DYKRLVVLSDDEVFLDDSDRIYKEFTEYLARPENKELPSVAYFSMEFGIHPSLKIYSG
                     GLGVLAGDYLKEASDSNVNITGIGLLYRYGYFRQKLGPRGEQQAIYEAEDFSQIPVEP
                     VKDSSGNQLTIQLTWPGRTVKARLWLAPVGKVKLYLLDTDFEDNTPEDRAITHYLYGG
                     DSENRLKQELILGIGGMRALLAIGIKPDVYHSNEGHSAFIGFERMRHLIEDEHLTFEE
                     SMEIIRASTLFTTHTPVPAGHDAFEEDLLRKYISHYHTRLNITWDQLMALGRCQDDHE
                     RKFNMSFLATRFSQEMNGVSQLHGHVSRGLFSRLWPGYLRDELYIGHVTNGVHYSTWV
                     APEWEQVYEKLTGKRHFDLSDREQWAKIYQLEDEKVYETKMKLKKRLFDNIRKRLQSD
                     MLERHVSPRTLMNISSHLNEKALTIAFARRFATYKRASLLFRDLDRLSRIVNNPERPV
                     QFIYAGKAHPNDGGGQDLIRRINEISQMPQFAGKVLFLENYDMDMARYLVRGADIWLN
                     TPTRPLEASGTSGEKAVMNGTIHFSVLDGWWVEGYHAFAGWALPKKRTFTSQELQDDV
                     DAETIYNMLEYEIVPAYYSKDESGIPREWTSYIKNTMVKVAPEFTTRRMLNDYMARYY
                     KKLWERSRRITADNFALARELAGWKGYISKVWDEIEIVKFDLKGLENNVFRSGHNYST
                     EIILDIKDIPVENVGVELVVTRQLKNGEHAFFSSREFKYVNRKKGRVIFSVDFQPEMA
                     GTFFYGIRIYARHSELPHRQDFCLLRWVD"
     gene            complement(19654..21672)
                     /locus_tag="PKK03_03715"
     CDS             complement(19654..21672)
                     /locus_tag="PKK03_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HNX83533.1"
                     /translation="MAERFYQVDPWLDPFREIIEARLTYISDREKLFTGGRTLTDYAN
                     GHHWYGLHRLPNGWVFREHAPNATSVILTGTFSDWKEDGRYALSRTAEGDWVGEFDAG
                     MLSHGDLYKLIVRWKGGHGERIPAYVTRAVQDDVTKIFSAQVWNPENGYVMKHKAPPP
                     PPSLLVYEAHVGMATEEERTGTYREFTENVLPRVKSLGYNTIQLMAIQEHPYYGSFGY
                     HVSSLFAPSSRFGTPDDLRELIDTAHKMGLRVIMDLVHSHAVANILEGLGLLDGDPGL
                     YFHTGSRRKHVAWDSLCYDYGKPEVNHFLLSNCKYWLEEFRFDGFRFDGITSMLYYDH
                     GLGSDFVSYKQYFDGNQDTDAIVYLTLANKLIHEFNHDATTVAEEMSGMPGLAASVAS
                     GGMGFDYRLAMGVPDIWIKMVKEIPDEEWDLGYLLHELTQHRAEEKVVSYCESHDQAL
                     VGDKTLIFRLADKEMYTSMSLLTPSLIVDRAVALHKMIRLFTFMAAGGGYLTFMGNEF
                     GHPEWIDFPREGNGWSYKYARRQWRLADDELLKYRFLNEFDRAMVETGKRHGLPRLQL
                     TESLSVNNSDKIIAFRRGDLVTVMNFNPTISFTGYGIPVKGKFRIILDTDDPLFGGQG
                     RIDTAQTYFSQPDQGHYGVTAPHHLKLYLPARTAVVLLQERVKTIMDL"
     gene            21742..22680
                     /locus_tag="PKK03_03720"
     CDS             21742..22680
                     /locus_tag="PKK03_03720"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNX83534.1"
                     /translation="MKVYHGYTGISFNHPVITMGVFDGVHLGHRMLLRRVTGEAAKDG
                     ADAVAVTFDPHPRIVLSGDTGHLSFLTDLEERTELLRETGIGHLVVIPFTAELSRMTA
                     PEFVSSILCRHLGVRHLITGYNHHFGRRHEGDSNTIIECAERMDFRVTREEAYMVEGE
                     AVSSSLIRKALGEGNIEKAASMLGYDYFIRGRVVSGRKIGRNMGFPTANIAPLFAHKL
                     IPRTGVYAVEATVEGDPDRHMAMLNIGHRPTITDSDGVRTIEVHLIDFEGDLYGKVVT
                     VTFRHRLRDEMKFESVDALSARLTLDREMTIALLGR"
     gene            22731..24146
                     /gene="ahcY"
                     /locus_tag="PKK03_03725"
     CDS             22731..24146
                     /gene="ahcY"
                     /locus_tag="PKK03_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNX83535.1"
                     /translation="MSFIINDLQYRVKDISLAGFGRKEIEIAEKEMPGLLSVRHKYSD
                     SKPLRGARITGSLHMTIQTAVLIETLRELGADVRWASCNIFSTQDHAAAAIAAAGIPV
                     FAWKGETLEEYWWCTAQALTFPGGKGPNLIVDDGGDASLLVHLGYKAEKDHSVLEAKP
                     ASREEAIVLDTLRRILEEDPQKWHRTVSEMKGISEETTTGVHRLYQMLEAGELLVPAI
                     NVNDSVTKSKFDNLYGCRESLADGIKRATDVMIAGKVVVVCGYGDVGKGSARSMRSYG
                     ARVIVTEIDPICALQAAMEGFEVRTVEESLAEGNIFVTATGNRDIITIEHMEKMKDQA
                     IVCNIGHFDNEIQVDALNAYKGISKINIKPQVDKYVFPDGHAIFLLAEGRLVNLGCAT
                     GHPSFVMSNSFSNQTLAQLELWTKDHAIGVYRLPKQLDEEVARLHLAQLGVSLTRMTK
                     AQSDYIGVPEEGPYKPDHYRY"
     gene            complement(24153..24605)
                     /locus_tag="PKK03_03730"
     CDS             complement(24153..24605)
                     /locus_tag="PKK03_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83536.1"
                     /translation="MLISLFTLPIISCDRDKNDVIPDITVDFTITASDPEFYDLFYAP
                     GTSVLISSAHRYLGQGTAGYDGNGIIIYNASEGLFYAYDRTCPHCFVTDDVSVAVDVD
                     GIFISCPRCSTDYAIGSSGLPLNGPGQYYLKNYRTSFTGYSVRVWNRR"
     gene            24697..25296
                     /locus_tag="PKK03_03735"
     CDS             24697..25296
                     /locus_tag="PKK03_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83537.1"
                     /translation="MKRGLVLLRFVSAVIVLLFVVQVTECNAQKNATKRFERETFGKS
                     RRSKPVKESGESKAAAKAMKEQARKEARREKEDEKALKQLRDRHYQIQSEETKMRMEN
                     NSRNTADKYKVKRQKQRREQTEPELKKPQQPAPDRAGPKPKTKDPSKQPEMKQQKQKA
                     KSKINDPSKQPVLKQQKKKAKTVNPKKQPKIKQHKVKKY"
     gene            25302..25943
                     /locus_tag="PKK03_03740"
     CDS             25302..25943
                     /locus_tag="PKK03_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83538.1"
                     /translation="MEDVGSLIFYIVLGLIALAGSFQGKGKKKQGTPKPAQRKPGTVT
                     TKPPVRTTAQTTRPAPSTMRQERPAPSTVRQERSVPKPSPWTIPAGQTLEGRYDEPMA
                     RDFSREGNIRDDLAGAFNSEGSIASGMGEAFAREGSIADSMAAAFASEGVSALGESAI
                     DEFIHTEISDSEIGDAPGYDYEALPGADIHKEGFDLKKAVIYSAVLDRKEYSR"
     gene            26064..26537
                     /gene="greA"
                     /locus_tag="PKK03_03745"
     CDS             26064..26537
                     /gene="greA"
                     /locus_tag="PKK03_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNX83539.1"
                     /translation="MMSEFVYVTEEGLQKMKEELDLLRNERPAIARMIADARDKGDLT
                     ENAEYSAAKEAQGMLEMKIARLEDMVARSRIIDGSRINTKTVQILNKVRILNKANGSL
                     MEYQLVPEGEANLKEGKIAATSPIAQGLMGRKVGDVVQVKVPAGTIPFEIIEISI"
     gene            26550..26948
                     /locus_tag="PKK03_03750"
     CDS             26550..26948
                     /locus_tag="PKK03_03750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNX83540.1"
                     /translation="MASIFTRIINGEIPCYRVAENDRYIAFLDVRPLKTGHTLVVPKT
                     EVDYIFDMDDEALSGMMIFAGKVARAMKEVIECKRIGVAVIGLEVPHAHIHLIPISRE
                     GDMLFTNPRVSLSPEENERLAKEIGARVKR"
     gene            complement(27046..27354)
                     /locus_tag="PKK03_03755"
     CDS             complement(27046..27354)
                     /locus_tag="PKK03_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83541.1"
                     /translation="MKKWMIGSLILIAMMFVAAGVYAQTTDKEKKHTKSECTFVDKNK
                     DSKCDVCGSTADACKKEAVKGKDCSKCPSAAECGDKKGEVVPAKEGTTATAPCCAGKK
                     "
     gene            27465..28175
                     /locus_tag="PKK03_03760"
     CDS             27465..28175
                     /locus_tag="PKK03_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNX83542.1"
                     /translation="MFHIKDIADDFLSLFLPRPCLSCGVPLVRGEEVLCTGCMLDMAR
                     TDFHLRRGNMLEQAFWGRCSVERAAAYSVYNRGSRIRKLIHLLKYSGRKDIGIMLGRL
                     YGSVLTGSGFTDGMDMIIPVPLDPARERRRGYNQSLCIAEGMASHCRLPVRSDILCRT
                     GKSGSQTKRGRYERWENVEGLFTVTRPLDIAGRHVLLVDDVITTGSTMEACVTALQSA
                     GGVMVSVAALAAAQKLTV"
     gene            complement(28192..28755)
                     /gene="ruvC"
                     /locus_tag="PKK03_03765"
     CDS             complement(28192..28755)
                     /gene="ruvC"
                     /locus_tag="PKK03_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNX83543.1"
                     /translation="MKPGQSEKIILGIDPGTTVTGYGLISVSGRSPSLITLGIIDLRK
                     KEDHFRKIRTIFEETLGLIDRYHPDEFAIEAPFFGKNVQSMLKLGRAQGAAIAAALYR
                     DLPVFEYAPRMIKMSITGRGQASKEQVASMLRTILAFHRESMVLDATDALGAALCHFY
                     QSNKPVAAREFRNWGDFIKKNSDRIKQ"
     gene            complement(28752..30086)
                     /locus_tag="PKK03_03770"
     CDS             complement(28752..30086)
                     /locus_tag="PKK03_03770"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX83544.1"
                     /translation="MKKILIISFLVIPFLFGCEKDPEPVKTPTLEQRARDGLYDLMKY
                     VYLWYTDMPTVKVSDYSGPEELLEALRYRQRDRWSFVTDFESFMASMEGSFVGHGIRM
                     GLDEMNNVRVVSLYEGSDLWPKGVRRGWIVKEINGTAIAPIFISGNNTAYNNLMGPGT
                     AGTTNSFKFINPDGQEVTYNSVKSSFIINSVTASKVLDLPGGKTGYLCFETFIDPSEQ
                     ELNDAFADFKASNVTDLIIDLRYNTGGYMDIAQQLASLVLAWTDTTKVAYKLTYNTLV
                     GPEWNESYKFIRTESPLGLDRVVFITTRSSASASEVVINSLLPYVDVKIVGDTTHGKP
                     AGMNIWGYPFPSDAEPEPDYKYVFAPITFEYLNAMDQGRFYDGIPPDVRAADDITHDF
                     GDPEEASLKAAIGVLNGTKAAPAVPVRRPRIWSEGNQLPTELFLGPPQAFRK"
     gene            complement(30133..31017)
                     /locus_tag="PKK03_03775"
     CDS             complement(30133..31017)
                     /locus_tag="PKK03_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX83545.1"
                     /translation="MKKGQQAYIYAALSVMLWGTIPTAFKIALSELSIVTMLGITTLV
                     STAVLFAIMFVTGKLCLLRQTTGRELLWSALLGLVNPVGYYLILLTAYSRLPGQVAQP
                     INMIWPIILVFFSIPLLGHRIPARSFLALAISLVGVYIISSQGAPLKPGRSDTLGVML
                     ALVSGVLWALYFVLNVRDRRDESVKLFTSFAFGSVYMLILMATGGSFTHALSIKGVAA
                     SVYCGFFEMGITFWLWLKALQLSETTDKVSNLVYFAPFISLILLHFIIDEPVYYTTPL
                     GLLLIIGSVIYQNRAKLA"
     gene            complement(31023..32297)
                     /gene="serS"
                     /locus_tag="PKK03_03780"
     CDS             complement(31023..32297)
                     /gene="serS"
                     /locus_tag="PKK03_03780"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNX83546.1"
                     /translation="MLTINLIREQSSFVIERLKVKNFDATEIVQHVLDLDRRRRELQA
                     ESDSIQAELNTLSREIGAMMKEGRRDEADAARQGIGAMKGKQKQLADTLEVVSGEMQT
                     ELVRLPNLPHALVAPGKSAEDNVVVRSGGAIPHLYEGAVPHWDLIKKYDIIDFDLGIK
                     ITGAGFPVYKGKGAKLQRSLINFFLDEGEKAGYREIEPPIVVNEDSGFGTGQLPDKEG
                     QMYHVGIDNLYMIPTAEVPITNIYRDVILDAAVMPVKNMGYTPCFRREAGSWGAHVRG
                     LNRLHQFDKVEIVQIAHPDKSYESLEEMVSHVEGLLVKLELPYRIVRLCGGDMSFTSA
                     MTYDFEVWSAAQQRWLEVSSTSNFEAFQANRLRCRFREKGAKQTTLCHTLNGSALALP
                     RIVAALLENNQTPGGITIPAALVPYTGFGMIS"
     gene            complement(32402..32662)
                     /gene="rpmA"
                     /locus_tag="PKK03_03785"
     CDS             complement(32402..32662)
                     /gene="rpmA"
                     /locus_tag="PKK03_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNX83547.1"
                     /translation="MAHKKGVGSSRNGRDSESKRLGVKLFGGQTVKAGSIIVRQRGTT
                     HNPGENVGLGKDHTLFALVDGTVEFKKKRDNKSYVSVKPVAE"
BASE COUNT         8127 a         8299 c         8588 g         7683 t
ORIGIN      
        1 aaagaaagtt gttcccgggg ggattgccat agcctgcctg cgcaaaacca aagagccttg
       61 gcatccgctg actcttcaaa aggtttttgg ataattccag ctgcaggcgg cgggtatcaa
      121 agagctgcag gtcgggattc tccaccttct cgtaaccggt aataaggttg tcaggcagcg
      181 ccagcccggc atatttcagt cctgacatcc cggtgagttg ttccagtgca agagccagcg
      241 catcatgcct tcgacccagc tcagcagctg cctgctcagc ctttatcatc tctgccttga
      301 gaacgtcaag tgtaactggc ggaaccacac cgttctccac tcctgattca gcctcactga
      361 tgcgggtgcc cagttccgag atcagtacag atgtcacttc agcctggctt cttgtgagca
      421 gcagggaaaa gtaaagattg tttacctgtt cgcgaaggcg gtatatgtcg gcttcgctct
      481 gctgcatgtt cagctcgctg accaccctct ccacctcgcg tgcgctgcgt gtcgccccgc
      541 cgtcccagat aacctggttg aggtcaaccg tggccctgta ctgctcatgc ggtattgacg
      601 gcagcgagcc cggaggtacc ggcatggagc caagcatctc agaaatgtcc accacatccg
      661 actggtagtt gaatgagccc cccaggtcca gggaaggaaa ccaggcggca gccaggttat
      721 ggtcacgcag ttgcgtcatg cccgcgtaga gttctttctc tccagagaga ggtgaaatga
      781 cggtggcaga gtcatagcac tgccacaggg taactgtccg ctgtggcatg agaggcaatg
      841 ccggaaggaa caatatcgtc agaaaggcaa gtctcttcat ttcttcctgt gttttaagga
      901 gttgaggatg aagtcggcgg caaattcctt acgttcatcc atgaatgttt taaacctcct
      961 gttgtcatgc atctggggca tcatcagggc tatcatgtcc cgtgccgcat aggggaatac
     1021 cgacatcgaa acaactgtga tcatcagttg cggcatatcg ctttttttaa tgccgtatcc
     1081 tttgagttcc tttgcatgct tctcatatat caggtcacgc atctctccgt acaggattgt
     1141 ctccttcagt cccgccgcaa tggacggatt ccttgagact tcattcagaa ggaagatagg
     1201 cagtttgggg tttttgctca ggaagtcaat gtgctgatca agaaactgcc ttatcttttc
     1261 cttgaaagtg gtgtctgcag caaaaattgt catgatcttt tcaaacatct tcttcagcaa
     1321 cacctggaat actgccataa agagctgatc cttactacgg aagtaatagt gcagcaacgc
     1381 cttgttaata ccggccctgt ctgcaatttc ctgcatcctg gcgccttcat agcccttctc
     1441 ttcaaaaata tctgaggctg ccctgaatat cttaagcctt gtctggctgt cagttactac
     1501 cgcctttgcc atagttatta actgtatagt ttaaccaaat gaattaacca acaggtcaaa
     1561 tttataaagt aatttaatta tttgcaagtg aattattgat tttttaaatt tgattgtcaa
     1621 tatctgtttc acaaaaagac aattgccatg aagaactttt ttaaaaaact gggtcgctgg
     1681 ctgctgggaa tagtattgtt cctggttgcc ctcatgattg ttttcattgc catgtcagca
     1741 tcttcaaaga agaaaatgac tccagctgac accggcctcc ttaccgaaga cgtctatgcc
     1801 gtaagggaca aatacgtcaa tatgtacctt gtcagggacg ggaatgacta catagccatt
     1861 gacgcgggca taaaacccgg taatatccgg ggtgaactga aaaagcttga cattgaccct
     1921 gaccgggtaa gggcagtgtt cctgacacac agtgacgcag atcacgcagg gggtatttcc
     1981 atgttcaaac gggcaactat ttacatgcac aggaatgagg agaagatgat taacggtgag
     2041 accggacgga tgctctggat aggcaacaac attgatgcga aggagtacac actccttgat
     2101 gaccataccg tcaggatcgg cggtctgagg ataaagccaa tccccacccc cgggcatact
     2161 gcagggctta cctgttacct tgtcaatgac atataccttt tcaccggtga tgctgtaagc
     2221 ctccgcgacg gtgtcatcgg gcttttcccg aaatacatca acaagtatcc ccgaaaggcc
     2281 cggagatcag ttcagaatat cctgaacctt gaaggtgtca tgtacatctt caccggacat
     2341 cacggcttca gcgccaacta ctcctccgcc gtccgcggtt acagggaata ggagcctgcc
     2401 ggcttccgca gaaggctgag atacccctca ttcccacccc gcggaaaacc tgcctctcct
     2461 attactctgc acactgctgc cggaagaaca aattttctgt catgttgtta ttccattaaa
     2521 aagtaacggc atgaaaacca ggacagcaga aaagatcata ccaccgcctc ccgtccattt
     2581 tgtcggtgac ggcttcaggg tgcacaactt cattccgggc gtctatccgc tcactatggc
     2641 caggatggat ccttttatac ttctggacta taactcaaag ttttatttct ctcccacggc
     2701 caaacccagg ggagtgggag tacatcctca ccgaggattt gagactgtca gcatagccta
     2761 caagggtcgc atagctcatc acgacagctt tgggaacagc ggcgtcatag gagagggaga
     2821 tgtgcagtgg atgacagcat catcaggact cctgcacaag gagtatcatg agaaggagtt
     2881 tgcacgtgca ggaggggatt tccatatggt tcagctctgg gttaacctgc cggcaaaaga
     2941 caagatgtct cctcctaaat accagccggt tacgaatgac cagatgggaa agtatatcct
     3001 gcacggcgat cagggcatta ttgaagtcat cgcagggtca taccgtgaaa cgaaaggacc
     3061 tgcctcaacc tttacaccaa tggcactgat gaatgccagg ctgaatgccg ggggaaaagc
     3121 ggattttgaa tttccttccc attacaatac tgccgccctg atcattgagg gccgggtact
     3181 ggtcaacggt tcggtgaagg ctcccgcaga taatttcgtc ctgttcgcca atgatggtga
     3241 acagttcaca atagaagcag aggaagattc catcgtcctg atcctcagcg gcgaaccaat
     3301 catggaacca attgccgccc acggaccttt cgtaatgaac acacgaaccg aaatctacca
     3361 ggctttcgag gatgtggaga acgggaaatt cggatacctg gaggaatagt ttaccgggaa
     3421 ataacattca tattattgaa ttacatttac agcagatttt taatccatgc ttaaaaacct
     3481 gctaatcatc ggatccggtg gcttcatagg cagtattgcc cgctacctgg tttcccacct
     3541 gaacctcact gtcactttcc attcaatacc ggttggtacc ctgctggtga acgtgaccgg
     3601 gagtttcctg atcgggattc tgaccggcat tgctgaaaaa agcatgatcc cgggcccgga
     3661 gtggagactc ttcctgatgg ttggcctttg cggcggattc actacctttt caactttcac
     3721 cggagagaac ctgatgctga tgcacaacgg tcagttactg acagtccttc tctatacagg
     3781 gctaagtatc ttcctcggat tccttgctgt ttacctgggc tatgtgacaa ctaatctcct
     3841 ctgatcatgg aaaagaaacc caattcggtt ttaaaagtgt ttgccagctc cactgacagg
     3901 cttggctctc agttacttta tgaggctgtt gtccttcgcg ccagagaagc tggaatctcg
     3961 ggggcaacag tttacagggg aatgatgggt tacgggctga gcagccatat ccactcttca
     4021 aagttctggg agcttacgga aaaactgccg gtggtagtgg aattgatcga tacggaagaa
     4081 aaattaagaa cattctatga ggtaatagcc cctgagctgg agtcgctacc caaaggctgc
     4141 ctggttacaa tttcacctgt agaagtgatc ctgcacaaat caggggagaa acagggaagg
     4201 agccggtaat tcgcggaatc tgacagactc tcgagaatac agagaacggg aagtttggcc
     4261 atcctgacga cttggggcta aaaatgcaaa gctccgttgg ggatgcggag aactgaacct
     4321 ttggccgccc tgacgactga caccgtggag ccagccctgg ttttcttcca ctgactgttg
     4381 catcttatat tccgattgct taaatttatg ctgcatttcc tccttttatt gtgcaacata
     4441 aagactgagt catgctggtc aagaccttca gcgctgccgt ttccggtatt gaggctgccc
     4501 cggttaccat tgaggtggat gtcacccgcg gtgtccgcta tatgcttgtc ggtttgccgg
     4561 acactgccgt caaggagagc cagcagcgga ttgaatcagc cctccgggct gtgggacttc
     4621 gctggccggg gaggcaggtg ataatcaaca tggcgcctgc cgatatcagg aaggagggtt
     4681 cgtcatatga cctgcccctg gctgtaggca tactggtggc ttcggagatg gtaagcggca
     4741 actgtcttga cggcatgctt cttaccggtg agctgtcgct cgacggggtt gtacggcctg
     4801 tccgcggagt gcttcccatg gcgctgcggg cccgcgagga gggcttcagg tgtatgatgg
     4861 tgccggcact caacgcgccc gaagcagctg tagtagaggg actggatatc tacggtgtca
     4921 atactcttgg cgaggttctg ggttacctga ggggtgacac tactccggcg ccggtcacag
     4981 tcaacctcag ggagcttttc agccgcgagg gaaatgtcta tgacactgat tttgcggatg
     5041 tgaagggcca ggagaatgtc aagcgcgccc tcgaggtggc tgtcgcagga aaccataatg
     5101 ccatcatgat tggtccgccg ggttccggga agacgatgct tgcccgcagg ctgcccggca
     5161 ttctcccgcc tcttaccctt gaggaggcgc tggaaaccac caggatccat tcggtggcag
     5221 gcaagatcga caatcacatt ccgctgatga cctgcagacc gttccgcagt ccgcaccata
     5281 catcgtctga catagccctt gtaggcgggg gccccgtgcc ccagcctggg gagatcagcc
     5341 tggcccataa cggcatactc ttcctcgatg agctgccgga gttcaaaaga agtgtactgg
     5401 aagttttgcg gcaaccgctg gaagacaggg ttatcaccat agcccgtgcc aggctgagct
     5461 gcaatttccc ggcctcggtg atgctcatcg cctcaatgaa tccctgcccg tgcggatttc
     5521 ataaccatcc ggtgagggaa tgcacctgcg cacccggaat ggtgcagaga tatatgagcc
     5581 ggatctcagg gcccctgctt gacaggatag acattcacat tgaagttgtg ccggctgact
     5641 atcagaatct ttcggtgaca gctaagacag aaaattcatc agtgataaga gaaagggtgg
     5701 ttaaggcccg ggaaatacag tcagaacgtt ttgccggcaa tgacggcata cactccaatg
     5761 cacagatgac cccggccctg atacgcagac actgccatat agatgacgcc ggaggaaggc
     5821 tcctgagggc agcgatggag aaacgcggac tgtcagcccg ctgttatgac cgcatactta
     5881 aggtagcccg gacaatagcc gacctcgacc actcggacac catcaggcct gaccacctgt
     5941 ccgaagccat caattacaga aacctcgacc gggagggatg ggcaggctga aatttatgcc
     6001 aactgtagct agtgacattt ctttttattt aatttgtctc cggggaatgt tggtacttca
     6061 ttttgtcgga atgtatggaa cttattaaca aattaaggca gaagctttca gacgaacatc
     6121 tgatgttcgc ctaccgggga gaggtgacag gagataactc cgtcagcctt ctgaccctgc
     6181 tggaacgtga gatggagttc tctgacttca gcatgctggg ccgcaaacgg ctcttcatgt
     6241 tcgtcctcga aaacctccag aacataacca gacacggcat cacccggagt gaagacatca
     6301 cctccctggt tgtttacagc aagacccccg acggctacac cgtcagcaca ggcaatgcgc
     6361 tcaaaaagag cgaggtcagg ggtctcagaa gtaaccttca taagatcaac agccttgatc
     6421 cggacaagat acgggaggtc tacagaacca tgctccagga taccaaaatc ggcaagaagg
     6481 gcggggcagg actgggcctg atggagatgg cccgcaagac gggaaacagg cttgactatg
     6541 atttctaccc gatagatgag aagtattctt atttcattct cagcaagacc atcgattccg
     6601 gcggtaaggg gatgacagag cccgcacagg atcagagcgc ttttgactct aaaaggatag
     6661 tgcaactgga gcggatgatg aagtcaaaca atatcttctt catctgggca gggcctatga
     6721 cccatgccat cagcaaggag ctgctgaact tcagcgagac aacaatgcgc gaggccgaac
     6781 tggaacagaa cctccacaag cgcgttttcg ccacactcat tgagctgctt cagaatgtgg
     6841 ctcagcatag cccgggcttt gaggctgaga agcactacgg catccccctg gccatgatac
     6901 gtaaaacatc ggcatcattc ataatcacct caggaaacct gatcaggaga agcgatactg
     6961 atcatcttac ccagaaactt gacatggtaa acaggtatga tgaggaaggg cttaagaaac
     7021 tgctcaaggt ggcgttgatg ggacaggata tgtcaaagat ttccaccggc tacatgggtc
     7081 tgctggaaat ggcccgcagg tcagggaaca ggcttaccta caagttcgac gatgtgaatg
     7141 acgaatatgc ctattatacc ataaccgtca gggtgagggc aaagggattc aagtcaccct
     7201 tggcgggtta atctgaagta atcttataat tgtcagaatt gccggcgatg ccggtaatga
     7261 aacatcattg cagggtgacc agcgtgagct cctttccgtc atacacggca taactctcac
     7321 ggcagctgaa ccagtcgccc aggattatca tccttctccc attctcatca tatgtcagtg
     7381 gcaggtgcct gtgcccgtag atgtagtact ttgccctgca tccgccggct gcaacacttc
     7441 ttgtgtgcct gacgaggtct tccttctcct cacccatgaa cggaagagat acatgcttgg
     7501 ccagcctgct gctctttgac catgcatgtc ctatagccac tccccagcga gggtggaggg
     7561 cagaaaagag aacacgtgcg atcctgctgc ggaagaggcc gaatagcagc ctgcctccga
     7621 tccttctgct tcccagtcct tcgccatggg ccatgtaaaa cagttcccct ccgatggtgg
     7681 tgatatgcgg ctcataatgc accgtcaccc tgcattcttc ggccaggtag cctttcatcc
     7741 acatgtcatg gttacctgta aagatatgta ccccgattcc gctgtcagtc agagaggcca
     7801 gcctgcccag aaaccttgtg aatcctttcg gaatcacctt cctgtattcc caccagaagt
     7861 cgaacacgtc gcccagcagc cagacagact ctgcatctgc tgacacccgg tcaagccatg
     7921 ctatcaccct ctgctcgcgt tcatgaggat cggtcccggc ggccagtccc aggtgaaagt
     7981 cagaagcaaa atatgttttc cggccttcca aatcgaatct attggttgaa taactggtcc
     8041 atcctgataa gcaaattgcg ccctgtcagg ttggtgttga ctatgttgcc ctgcgggtca
     8101 taaagcaggt ttgaggggag ggaggtgata ttcatcagcc gtgcataaat cggatttgcc
     8161 gggtcaggtt ccctgacgct gatccacggt atctcctcaa agcgtaccac gcttttccat
     8221 ctctcggggt ccgtgtcgag actgacctgg tagatttcca gtcctttcct gtgatagtcg
     8281 ttgtaaatcg acctgagaag cggcagctga ttcatcgaag cctccgaagt ggtgctccag
     8341 aatgagacca gcacatattt tcccttcaat gacgaaacac cgaccatttt gtcgttgatg
     8401 tcaggaagtt ccggtgagga cttggtttcc ggcaattgca tggcaaggtc ggagattctg
     8461 tcaatataca tctggttcag ctccgaggtg acgttttcct taagtgcgcg cacatggcgc
     8521 gagaccggat accggaccga cagtgagtcg gatacgatct tcaggtactg aaggtcgcgg
     8581 ggctgataaa ggacataggt gttttcgtca agcctctggt aaagagcctg tattgacgac
     8641 attgagctta tatgctccac cacgaattta atgttgaact tccgctgtgc ctcaacagct
     8701 tccgtgtatg tcttctgaag ctcagggccg cggacagcca gatcatcgat gctgagggca
     8761 ttgtagatat ttctgactga gtcgagcctg gttttggtca ccagcagctg ctggtcaagc
     8821 agccgtatat ctgccgatcc gggtgagccc tgcacggtgt aattcattgc aactggcgtt
     8881 ttcccgagtt cgagggagat attctccccc ggcattacca gcagcgccac gaactcattg
     8941 ccggggaatc tcaactggta gaactccggg ccggtcactt cggtgacgaa tttaaaccgt
     9001 cccctcttta tttcggttga gtcgaccctg gtgctccggt ccacttcaga ctggtcaagg
     9061 tataccatac cttctgccgg cacggtaaag gcccccttca ttatcacttt attatcgttg
     9121 ttgcagcctg ccagaagaag tgcggccagg agcacaggca aaaatctctt cattcctttt
     9181 tgatatttcc ggtacaaatt aaattaaaaa tcagcattgg ttactgtgcc gccagcagtt
     9241 cctccagctt tttctgaagg tcatcgcccc tgaggttaac ggctgtcacc cttccttcac
     9301 ggtcaatgag gaagctggcc ggtatttcgg tcaggccaaa ctgcttcacc acttccgagt
     9361 cgcggtaacg gaggtcactt acatgcttcc agcgtcccag cctgtcattt cttattgcct
     9421 cgagccagtc ctccttcctg aggtcaagtg agacctggaa gatatcaaat cccttccagt
     9481 ggtacatgtc atagagtctt accaggttgg gattctcctc gcggcatggc gggcaccatg
     9541 cagcccagaa atcaaccagc accacccttc ctctcaaaga tgacaggctg agcgtgtcac
     9601 cgtcagggtc cgggaggact atgtcaggga gcaattcacc gggcaccgga ggctcgccgg
     9661 cctgtctgcc cacggagacg ctcttgcgca gaccggctac gaacgaatgc aggtcaagca
     9721 ccaggtcaga ctcagggtaa agggcataca atgtcgaatc gacactgtaa aagagctcag
     9781 ggttctttgc cggttcaaag agctccagtc ccggcactac atgctggttc agcaggtaaa
     9841 ccgggatcat tgacgacctg ttttcgttaa ggagagtcaa agcccttgca cggaagccgg
     9901 ccacaatatc ggcagcacgc ctgtcgaggc tgtccatcag cagcggacgg cgggggctgt
     9961 cgatactgtc attgtaggta tcggtgagtt tcttaagccc ggcaaccacc tcatcctggt
    10021 ccttcctgaa gccgagaata accgaagtgc cctcggatcc agtcaccgag gatggtctgg
    10081 ccattgaatt ccgcgcagct ttcacctcta ttttttctcc cggtgcgatg agcatggtga
    10141 gaaagtcatc atcatccatg ctgagcatga aaaaggccgg ccacccggtc tccgacctga
    10201 agctgaacct tccgtccttc cctggtacca ccgagtcaac cggttccagg atgcctggct
    10261 taacctcctt cagcagcaga tattcaccat ctccggcacc atctattgtc ccgctgatac
    10321 tgaaacttcc acgcttgcat ccggatatca gaacagaagc acaaaacagc atcagaatta
    10381 cttttttgcc catattgtaa ggtattacat aaaacaactt taaaggtccc tgttaaattt
    10441 ccgtcattcc ggtcgaacca ttttacctcg cattggttct ccttcaggag tgcaaagata
    10501 tctatttttc agagccttta tggacaggct tgtcatcagc cggcacataa ttaatctgat
    10561 ttttttgctt ttctttgcac ctgaaacgat ttgttattaa attaatagag ttaattcttc
    10621 tgcttttctt tagcagcaaa acagaactcc tgaaaatatg ggatacatta aatttgacaa
    10681 ggcacaggta gttaacctgg aatattccct gacccgcgaa attctgcgaa gcaacagggc
    10741 cggaagctat tcgtccacta ccatagtcgg atgcaatacc cgcaaatatc acggctggct
    10801 tgtatgcccc gttgatgcca tgggaggcga aaggcatgtc ctcctctcat cgatcgactc
    10861 cacagtagtc agtaacggtc agagcttcaa cacaggtatc cataagtatc atggtgacca
    10921 ctacagcccg aagggacaca aatatgtgga ggactttgat attcaggata ttcccgggat
    10981 gacctacagg gtgggcaatg taatcatgaa gcaggagcgc atcctggtcc attacgagga
    11041 gcaactgctg gtaaggtaca ccatcattga cacggatgag cctgtaattc tccagctgag
    11101 gccgttcctg accttcagga gcattcatca gctgacccat gccaatatgt gggccagtac
    11161 ccggaccgag ccggtgcctc acggtgttaa gatcaggatg tatgaaggct tcccttacct
    11221 tcatatgcag ctgtcatgca aggctgagtt cgtgccggtg cccgactggt acctcggggt
    11281 agagtatgtt gaggagcaga aacgcggata tgactattcg gaagatctgt ttgtcccggg
    11341 ctttttcgag atgaccgctt caaagggaga cgttatagtc ttttccattt caaccaggga
    11401 agagaagcat gcagggctga aggccaaatt cacccgcaca gtagccacca agattccgcg
    11461 aagcaacttc ggcaactgcc ttcgcaatgc tgcacagcag ttcattgaga agcgcggaaa
    11521 tgacaccaac attatcgcag ggtatcactg gttcgggtcc tggggacgtg acaccttcat
    11581 ctcattgccc ggtctggcgc tggccaggaa atcagaagat ctttacgcag cggtgattga
    11641 cacgcaggta cgccggatga agggcggcct cttcccgaac atgggtgaca atgacaatcc
    11701 tgcattcaac agcgttgatg caccgctctg gttcttccag gcactgtgga actacggact
    11761 tgatcctaaa gagacatgga agcggtacgg tgctgctatg aaggatgtgc tcaatgccta
    11821 cagggccgga acctccttcg gcattcacat gagggaaaac ggcctgatat actcaggcgc
    11881 tccgggcaag gcacttacat ggatggatgc cgtggtgaac ggtgttccgg tgactccgcg
    11941 tgaaggctat gcagttgaga taaatgccct gtggtacaat gcggtttgct attcgcttga
    12001 ctgtgcccgg ggcgcacgtg acagggcttt cgtgaaggag tgggaaaaac tgcctgacct
    12061 gatcagcagg tcattcatcg aaaccttctg ggatgaggag cacggatacc tggccgatta
    12121 tgtgaatgac agggagatga gaaacatgca gatcagaccg aatatggtca tcgccacctc
    12181 gcttccgtat gccatgctga cacgtgacca gatgaagagc atcctggata ttgtaaacag
    12241 gatccttgtc acaccgaggg gtttgcgcac actttcgccc tcggatgagg gatataaggg
    12301 tatttattgc ggcgaccagg ccacgcgtga caatgcttac caccagggta ccgtatggcc
    12361 gtggctgctc ggcccgttct gcgacggatg gctcagggtc tacggcgagg gcggcgtatc
    12421 acgcgtcaga aagcttataa cgggattcga ggaaacactg actgaagccg gcatttcaac
    12481 catctcggaa atctacgatg gtgatccgcc gcattcacca aggggggcga tatcacaggc
    12541 gtggagcgtg ggagagattc tccgcatctt caccctgctt gaaaagaatt atccagaaaa
    12601 taacaaatga tatgcgtgtc ctgatgtttg gatgggagtt ccctcctcat atagcagggg
    12661 gattgggaac agccagcttt ggcctcaccc gcggtctggc agcccaccgc gatattgaag
    12721 tcatcttcgt ggtcccgaaa gcttacggtg atgaagacca gagcattgtc agactcattg
    12781 gtgccaacac cgtgcccgtg gccttcaaga aggttaaata caggtatttc cagagacctg
    12841 tggaaaaaat tgaggtcttc tccaaactgg tgccatatac tgaccccgag gagttctgga
    12901 agataacccg cggcgaggtg gctgataccg atgtgctcgt caggacaaat gagcacggca
    12961 aggttgactt ctcgggaacc tacggccctt ctcttatgga tgaaattcac aaatattcgg
    13021 tggtggccca ggtaattgcg tcagaacagg agtttgatat cattcacgcg catgactggc
    13081 tggcttatcc ggcaggaata gctgcaaagg ccgtctccgg caagccgctg gtcatccatg
    13141 tgcatgccac tgacttcgac cgcagcggag gaaatgtcaa ccctgtggta tacggcatgg
    13201 aaaaggaggg gatggatgca gctgacaaaa tcattactgt cagcaacctt acccgtgaca
    13261 ttgtaataaa caaatacaac gttgacccgg aaaaggtcga gacggtttac aatgccgtgg
    13321 aaccgctgca tcattctgag ctcgatgtcc ccgagagggc ctatgatgac aagatagtta
    13381 ccttcctcgg ccgtatcacc atgcagaagg gtccggaata ctttatcgag gctgccagga
    13441 tggtcctttc gcgaatgcca aacgtcaggt tcgttatggc cggctctggt gacatgatgg
    13501 aacggatgat gcgtcatgcc gctgcactga gaattactga caggttccac tttaccggct
    13561 tcctgcgtgg caatgatgtc tttaccatgc tgagaatgag tgatgtatat gtgatgccat
    13621 cggtttcgga gccattcggc atatctccgc tagaagccat gcagtcaaac gtgccggtaa
    13681 taatcagcaa acagagcggt gtggcagagg tgcttacgca tgcagtgaaa gttgatttct
    13741 gggacattga agcaatggcc gatgccattt atggcatcct gacctatccg gctctctcga
    13801 ggatgttcat catgaacggc aaggaagagg tcgacaagct caagtgggaa aaggccgccg
    13861 gccatgttaa gggtatttat tcagaggttt tgcaacagac aagataaaag atatgagcac
    13921 atcacagaaa aaggcaattt gcctttactt ccaggttcat cagccattca ggctcagacg
    13981 gtaccgtttc ttcgagatgg ggcatgaaca ctattactat gacgatttca tgaatgagtc
    14041 tatcctgcgg aagatagcca caaactgcta cctgccggca aacaggataa tccttgacct
    14101 tgtaaaaaag cacaagggaa agttcagggt cacattctcc ctgacgggaa tcgttattga
    14161 ccagttcagg ctatatgccc cggaggtgct cgcttccttc agggaacttg cagaaacggg
    14221 atgcgttgag ttcctggccg agaccaactc acattcactg gcatcactca tgagcaggga
    14281 gagatatgag cttcaggtaa gggtgcaccg tgaaaaaatg atggagtata tcgggtatga
    14341 accaacttcc atccgaaata ccgaacttat ctattccgac gagattggaa gctggatggc
    14401 agaattgggc tacaaggccg ttgtaacaga gggggctaag catatccttg gctggaaatc
    14461 acccaactac ctttactgca atgccgttaa tcccaggctg aaggtcctgc tgagaaacta
    14521 caccctcagt gatgacatag ccttccgctt ctccagcaaa gagtggtcaa gctatcccct
    14581 gacagccgag aaatatgccg gctggataaa aggacttgca tctacctccg aactgattaa
    14641 tgttttcatg gattacgaga ccttcggaga acatcagaaa aaggagacgg gaatatttga
    14701 tttcctgaaa catttacccg aaacaataat aaagaaaacg ccatttaatt tcatgacggt
    14761 ttcagaggtg gcagacaact atcagccggt gtctctcctg catgttccgt cacccatatc
    14821 atgggctgac gaggagaggg acctcaccgc atggatgggc aatgagctgc agacagcagc
    14881 cgtaaacaag ctgtacagcc tttccgacag ggtgggcaga tgcaataatg aacagctgat
    14941 gcgcgactgg gtttacctgc agtcgagtga ccatttttac tacatgtcaa cgaagttctt
    15001 ctctgacggc gctgttcatg cctatttcaa tccttacgag aacccgtacc aggcattcat
    15061 gaactacatg aacgtgctga gtgacttcga gataagactc aatacagtct gtccgctcaa
    15121 cattcacgat gaggaggtca accgcctctc agcggatatt aagaaacgtg atcacatgat
    15181 caccgagctt caggagaaat tggccaggct tgaaaagaag ccggtggtaa gaacaaagcc
    15241 gggggccgca gcatcaaaaa agactgcccc gaagacagcc gcaaaaagca agaccgttgc
    15301 tgcaaaggca gtcacgaaaa aggttgccgc caggaagaaa tccggccgca aataataacc
    15361 ggggtttaaa tctatgtgca acataactgc gcctcgtgcg tattaattga ttattaatta
    15421 tatggaaatg aaagctgatt ttttattcga aacaagttgg gaagtatgca ataaggtagg
    15481 aggaatttat acagtcattt caaccaaggc gcttaacgta acctatgaat ttggagataa
    15541 ctatatactg atcggccctg atgtatggag ggacgacaag tccaatccgg agttcacacc
    15601 tgatgacacg atgttccagt catggaagac cgtggcggca caggaggggc tgatagtaaa
    15661 aaccggtcgc tggaacatca agggcaatcc gcgcgtaatg ctggtcgatt tcacgcccta
    15721 cttcggacag cagaatgaga tcttcgcccg tttctgggag acatacaagc ttgacagcat
    15781 ttcaggccag tgggattaca ttgagcctgc attattcggt tacgctgcag gtaaagtcat
    15841 cgagagcttc accaacttct accgtgaata tccgcgggtg attgcacagt ttcatgaatg
    15901 gatgaccggc atgggtgttc tctaccttga gaagcatgcc ccctgggttg ccacggcatt
    15961 caccactcac gccactgttc tcggtcgcag catagcaggc aacaacaggc agctctacag
    16021 tcgccttaag gagtttaacc cggtgcagac agcgcacgag tttaatgtca cctcaaaaca
    16081 gtcacttgaa aagatcagtg ctcaggaagc agatgtcttc acctcggtaa gtgagattac
    16141 cgggcgtgaa tgtgaggcgt tcctggggaa gaaaactgac gtgataaccc ctaacggctt
    16201 tgaggatacc tttgttcccg cccctgagaa ttttgatgag gcacgccgtg cagcacgtga
    16261 caggctcctc tcggtggcac aggcgctgct tggctataac ctgccggccg acaccctact
    16321 cgtctccacc agcgggaggt acgagtacag gaacaagggc attgacctct tcctctcggc
    16381 gctgggcgaa gtgaacagga agactgattc cagccgccag tgtgttgcat ttatcctggt
    16441 gcctgcatac cacaagggcc ccaggcagga tcttgccgct gcccttgcca atggtaccat
    16501 agttaccgaa ggcgacaaat atctgaccca cggactccat tatgtgaatg atgacccggt
    16561 gatgaaccaa ctgatacgcg agggcataca caacatagcc tccgaaaaag taaaggtcat
    16621 atttgtcccg tcatacctta acggtaatga cggtatcttc aacatggaat actatgattt
    16681 gctgatcggg ttcgacctta cactctatcc ctcttattat gaaccatggg gttacacacc
    16741 tcttgagagc ctgatgttcc atgttccgac cgtgacaacc aaccttgccg gattcggtat
    16801 gtgggtcgac acctatttca aggaacccgg caacggatgc ctggtactga accgtactga
    16861 caacaatgag aaggaggtca taaccggcat ggaggaattc atacgcaagt tcatgcatat
    16921 gaatcccgaa gaagctgccg ctgcaagcca gaaggcatgg gagatatccc ggatagccaa
    16981 ctggaacact ctgttcagct attacagcga agcataccgt tttgcacttg agaagagcga
    17041 ggaaagaaga gaccagccca gggattacac ccgcatactc gaagctaccg aggtacagat
    17101 gaggaaaccg ttccaggctc cggtctggaa agacatttac gtgcaatcag agttgccgga
    17161 acgcattgcc tatctcaagg aactatcctc aaacctctgg tggtcatgga acagcgaggc
    17221 tgagttcctt ttccgccgga tggatcccac cctgtgggag gaggttgggc acaatcccaa
    17281 gagactgctg gaagccatcg attacaagag gcttgtagtg ctttcagacg acgaggtgtt
    17341 cctggatgat tctgacagga tctataagga gtttacagaa tatcttgcca ggcctgaaaa
    17401 caaggagttg ccctccgtag cttatttcag catggagttt ggcatacacc caagcctgaa
    17461 gatctattct ggcggcctgg gtgttcttgc cggcgactat ctgaaggagg caagtgactc
    17521 caacgtgaac atcacgggca tcgggttact ctacaggtac ggttatttca ggcagaagct
    17581 gggtccgagg ggtgaacagc aggctatata tgaggctgag gacttttcgc agattcctgt
    17641 tgaaccggtg aaggacagta gcggcaatca gcttaccata cagcttacat ggcccggacg
    17701 taccgtgaag gcccggctgt ggctggcgcc ggtaggcaag gttaagcttt atctcctcga
    17761 cactgatttt gaggataaca cacctgagga cagggcaatc acccactatc tctatggcgg
    17821 tgactccgag aaccgcctta agcaggagct gatcctcggc attggaggaa tgcgggctct
    17881 gcttgccatc ggaataaaac ctgatgtcta tcacagcaac gaggggcatt cagcattcat
    17941 aggatttgag aggatgcgtc atctcattga ggatgagcac ctgacctttg aggagtcgat
    18001 ggaaattatc agggcatcaa ccctgtttac aacccatact cctgtgccgg caggccatga
    18061 tgcctttgaa gaagacctgc tgaggaaata tatttcacat taccacacca ggctgaatat
    18121 tacatgggat cagctgatgg ccctcggccg ctgccaggat gatcatgagc gcaagttcaa
    18181 catgagcttc ctcgccaccc gcttctccca ggagatgaac ggagtgagcc agcttcacgg
    18241 gcacgtttca cgcgggctgt tctccagact ctggccaggt taccttcgtg acgagcttta
    18301 cataggtcat gtcaccaacg gtgttcacta ttcaacatgg gtggctcccg agtgggaaca
    18361 ggtctatgag aaactcaccg gcaagcgtca ttttgacctg agtgaccgtg agcagtgggc
    18421 aaagatatac cagcttgaag acgagaaggt ttacgagacc aagatgaagc tcaagaagcg
    18481 cctgtttgat aacatacgca agcgcctcca gtcagacatg cttgagaggc atgtcagccc
    18541 gcgcacactg atgaacatca gctctcacct caatgaaaaa gccctgacaa ttgcatttgc
    18601 caggagattc gcaacataca agcgtgccag cctgctgttc cgtgacctcg accgtctctc
    18661 gagaatcgtc aataatcccg agagacccgt gcagttcatt tatgccggca aggctcatcc
    18721 aaatgacggc ggcggacagg atctcatcag gagaatcaat gaaatatcac agatgcctca
    18781 gttcgcgggg aaagtgcttt tcctggaaaa ctatgacatg gatatggcac gttaccttgt
    18841 acgcggtgca gatatatggc tgaatacgcc aacaagaccc ctggaagcat cgggtaccag
    18901 cggcgaaaaa gccgtaatga acggaaccat ccacttcagc gtcctcgacg gatggtgggt
    18961 agaaggttat catgccttcg ccggatgggc actgccaaag aaacggactt ttaccagcca
    19021 ggaactgcag gatgacgttg acgccgaaac catctacaac atgctcgagt atgagatcgt
    19081 tccggcctac tactcgaagg atgaaagcgg cataccacgc gaatggacca gctacatcaa
    19141 gaacaccatg gtcaaggtgg ctcccgagtt taccacccgc cgcatgctca acgactatat
    19201 ggcaaggtac tataagaagc tgtgggagag aagccgcagg ataacagctg ataattttgc
    19261 actggcacgc gaactggccg gatggaaagg ctatatctca aaggtatggg atgaaatcga
    19321 gatagttaaa ttcgacctta agggtctgga aaataacgtc ttccgttcgg gacacaacta
    19381 ctctaccgag atcatactcg atataaagga tataccggtg gagaatgtcg gggttgaact
    19441 ggtggtgact cgccagctca agaacggaga acatgccttc ttctcgtccc gcgagttcaa
    19501 atatgtcaac aggaagaagg gacgggtcat attcagcgtt gatttccaac cggagatggc
    19561 cggcactttc ttctacggaa tccgtatcta cgccaggcac agtgaactac ctcacaggca
    19621 ggatttctgc ctgctgagat gggtcgactg attctataga tccatgattg tctttaccct
    19681 ctcctggagg agcactacag ctgttcttgc agggaggtaa agcttcaggt ggtgcggagc
    19741 cgtcacccca taatgtccct ggtcaggttg tgagaagtag gtctgggctg tgtctatcct
    19801 gccctgtccg ccgaacaggg gatcatcggt gtcaaggatg atcctgaatt ttcccttgac
    19861 gggaattccg tatccggtaa aggaaatcgt cggattgaag ttcatcactg tcacaaggtc
    19921 gccgcgtctg aatgcaatga ttttgtcaga attgttgact gacagtgact ctgtcagctg
    19981 cagccggggc agcccgtgcc tcttgccggt ctcaaccatt gccctgtcga attcattcag
    20041 gaacctgtac ttcagcagtt catcatctgc cagcctccac tgcctgcgcg cgtatttata
    20101 gctccatccg ttgccttccc tggggaaatc aatccattcc gggtggccga attcattacc
    20161 catgaaggtg aggtaaccac cgcctgctgc catgaaggtg aagagcctga tcatcttgtg
    20221 gagagccacg gcccggtcaa ctatcaggct gggggtgagg aggctcattg aagtatacat
    20281 ttccttgtct gccagcctga aaatcagggt cttgtcgcct accagggcct ggtcatgaga
    20341 ttcacagtat gataccacct tctcctcggc ccggtgctgg gtgagttcat ggagcaggta
    20401 tccgagatcc cattcctcat cagggatctc ctttaccatc tttatccaga tgtcaggcac
    20461 tcccatggca agcctgtagt caaaacccat cccgccggag gcaacggagg cggcaagtcc
    20521 gggcatgccg ctcatctcct cggccacggt ggtggcgtca tggttgaatt catgtatcag
    20581 cttgttggca agtgtcaggt agacgatggc atccgtgtcc tggttaccgt caaagtactg
    20641 tttgtaagaa acgaagtcgg atcccagccc gtgatcatag taaagcatgc ttgttatgcc
    20701 gtcaaaccgg aatccgtcga agcggaactc ctcaagccag tatttgcagt ttgacagcag
    20761 gaaatggttg acctccggtt tgccgtagtc atagcagagt gaatcccacg ccacatgttt
    20821 cctccgtgaa ccggtatgaa agtacagccc gggatcaccg tcaagcaggc cgagcccctc
    20881 gaggatattt gccacggcgt gtgagtgaac caggtccatt attaccctca gacccatttt
    20941 gtgggcggta tcaatcagct ccctgaggtc atcgggggtg ccgaagcgtg atgagggggc
    21001 aaaaagactt gagacatgat agccgaatga gccgtaatag ggatgctcct ggatggccat
    21061 gagctgaatg gtattatatc caaggctttt cacccgggga agcacatttt cggtaaactc
    21121 ccggtatgtg ccggtccgtt cttcttcggt ggccatcccg acatgtgcct cgtaaaccag
    21181 aagtgagggt gggggcggag gtgccttgtg tttcatcacg tacccgtttt cagggttcca
    21241 cacctgtgcc gagaagatct tggtcacgtc atcctggact gcgcgggtga cataggccgg
    21301 aattctttct ccatggcctc ccttccatct gacaatcaac ttgtacaggt cgccgtgcga
    21361 aagcattcct gcatcgaact cacccaccca gtccccttct gccgtacggc tgagggcata
    21421 cctcccgtct tccttccagt ccgaaaatgt cccggtgaga atgactgaag ttgcattcgg
    21481 ggcatgctcc ctgaagaccc atccatttgg caagcggtgc agaccgtacc agtgatgtcc
    21541 gttggcatag tcggtcaggg tacggccgcc ggtaaacagt ttctcgcggt cactgatgta
    21601 ggtaagccgc gcttctatga tctccctgaa gggatcaagc cacggatcaa cctggtaaaa
    21661 tctttctgcc atattctttg ccctgatgtt acagtcaata taaagatagg attttattat
    21721 ttttgatgtc acaatccggt catgaaagtc tatcacggtt atacaggcat ctcattcaac
    21781 catccggtta ttacaatggg tgtctttgac ggcgttcacc ttgggcacag gatgcttctg
    21841 cggcgtgtga ccggggaggc cgcaaaagat ggtgctgatg cagtggcagt cacctttgat
    21901 ccccatcccc gcatagtcct ttccggcgat accggtcatc tgagcttcct gactgacctt
    21961 gaagaacgga cggagctctt acgggagaca ggcataggcc atcttgtggt catccccttt
    22021 acggctgagc tcagcaggat gaccgccccg gaatttgtca gttccattct ttgccgtcat
    22081 ctcggggtcc gccacctgat caccgggtat aaccaccatt tcggaaggcg ccatgaaggt
    22141 gacagcaaca caatcatcga gtgcgcggaa aggatggatt tcagggtgac gcgcgaggag
    22201 gcatacatgg ttgaagggga ggctgtgagc tcatcgctca tcaggaaggc gctcggggag
    22261 gggaacattg aaaaggccgc atcaatgctt ggttatgatt atttcatcag gggccgggtg
    22321 gtttccggaa gaaagatcgg ccgcaacatg ggtttcccca ctgccaacat tgctcctctg
    22381 tttgcacaca aactcattcc ccgtacaggg gtctatgccg tggaggcaac cgttgagggt
    22441 gacccggaca ggcacatggc catgcttaat atcgggcacc ggcctaccat cactgacagt
    22501 gacggggtac gcaccatcga ggttcacctt attgactttg agggtgatct gtacggaaag
    22561 gtggtaaccg taacattccg tcaccggttg cgtgatgaga tgaagttcga aagtgtggat
    22621 gcactctcag cccggctgac cctagaccgt gagatgacta tcgcactgct gggccgctga
    22681 acaataatcc ctttttttag ttatctttgc acacctaaaa attcattaac atgtcattca
    22741 tcatcaacga tcttcagtac agggtaaagg atatatcact tgccggattc ggccgtaaag
    22801 agatagagat tgctgagaag gagatgccgg ggttgctctc agtcaggcat aagtattcag
    22861 actccaaacc tctcaggggt gcaaggataa ccggctcgct ccatatgacc attcagaccg
    22921 ccgtcctcat tgagactctc agggagcttg gcgctgatgt cagatgggca agctgtaaca
    22981 tcttctccac gcaggatcac gccgctgctg ccattgccgc tgccggcatc ccggtatttg
    23041 catggaaagg tgagacgctc gaggagtact ggtggtgtac cgcacaggcc ctgaccttcc
    23101 ccggcgggaa aggcccgaac ctgattgttg atgacggagg cgacgcatca ctgctcgtcc
    23161 atctgggata caaggccgaa aaggaccact cagttcttga agcaaaaccg gcaagccggg
    23221 aagaggcaat agtgcttgac acactgagga ggatactgga ggaggacccg cagaagtggc
    23281 acaggacagt cagtgagatg aagggcatat ccgaagagac aaccacaggt gtgcatcgtc
    23341 tttaccagat gctggaggca ggtgaactgc tggtgcctgc aatcaatgtc aatgattcgg
    23401 tcaccaagag taaattcgac aatctctatg gctgccgtga gtcacttgct gacggtatca
    23461 agagggcaac ggatgtcatg atagcaggca aggttgttgt cgtttgcgga tacggtgacg
    23521 tcggcaaggg ttctgcaagg tcaatgaggt cgtatggcgc cagggtcatt gtgaccgaga
    23581 ttgatcctat ctgtgcactc caggctgcaa tggaaggctt cgaggtaagg acggtagagg
    23641 agagcctcgc cgaaggaaac atattcgtca ctgcaacagg caaccgtgac atcatcacca
    23701 ttgaacacat ggagaagatg aaggaccagg ccatcgtctg caacataggg cactttgaca
    23761 acgaaataca ggttgatgcg ctgaatgcat acaaaggcat cagtaagatc aatatcaagc
    23821 cgcaggttga caagtatgtg ttccctgacg gccatgccat tttccttctt gccgagggca
    23881 ggcttgtaaa ccttggctgt gccacgggtc atccatcatt cgtgatgagc aactcattca
    23941 gcaaccagac gctggcgcag cttgagcttt ggaccaagga ccacgcaatc ggtgtttaca
    24001 ggcttcccaa gcaactcgat gaggaggtgg cacgcctgca tcttgcacag ctcggcgtaa
    24061 gccttaccag gatgaccaaa gcccagtctg attatatagg cgtccctgag gagggtccgt
    24121 acaagcctga ccattacagg tactagaaca gcctatctcc tgttccagac cctcacactg
    24181 tagccggtga aggaggtgcg gtaattcttg agataatact gaccgggccc gttcaggggc
    24241 agacctgatg atccaatggc gtagtctgta gaacatctcg gacaactgat gaatatcccg
    24301 tccacatcaa ccgcaacact tacgtcatca gtgacaaaac aatgaggaca tgtgcggtca
    24361 tatgcgtaaa agagaccttc gctggcattg tagatgatga tgccgttacc atcgtaaccg
    24421 gcagttccct gccctaagta cctgtgcgca gaggagatca ggacgctggt gcctggagca
    24481 tagaagagat cataaaactc cggatcggaa gcggtgattg tgaagtcaac cgtgatgtca
    24541 gggatcacat cgttcttatc cctgtcacag gagattatag gcagtgtgaa cagagaaatg
    24601 agcaaaaaaa tccttaaatt tgaaactgac atgggcttgc ttccgtttct gtaaatgtag
    24661 ttatttccag tctgacgcgg ctaactgaaa aacgaaatga agagagggtt agtattatta
    24721 aggtttgtat cagcggtaat cgttctgctt tttgtggtgc aggtaaccga gtgcaatgcc
    24781 cagaagaatg ccactaaacg gtttgagagg gagaccttcg gcaaatcgcg tcgcagcaag
    24841 cctgtgaagg agagcggtga gagcaaggca gctgcaaaag cgatgaagga acaggccagg
    24901 aaggaagcaa ggcgggaaaa ggaagatgaa aaagccctta aacagctgag agacaggcat
    24961 taccaaatcc agagcgagga gacgaagatg cggatggaga acaacagcag gaacactgct
    25021 gacaaataca aggttaaaag gcagaaacag cgcagggagc agacggagcc ggagctgaag
    25081 aaaccacaac agccggcgcc tgaccgggcc ggaccaaagc cgaagaccaa ggatccttct
    25141 aaacagcctg aaatgaaaca gcagaagcag aaggcgaagt cgaagatcaa tgacccctcg
    25201 aaacaaccgg tgctgaagca gcaaaagaag aaggcaaaga cggtaaatcc aaagaaacag
    25261 ccgaagatca agcaacacaa ggtaaaaaaa tactgaagca gatggaagat gttggcagcc
    25321 tgatttttta tatagtgctg gggcttattg ccctggcagg atcattccag ggtaagggca
    25381 agaagaagca gggcacaccg aaaccggcgc agcgcaaacc gggtacagtt accacaaagc
    25441 ccccggtgag aaccacggca caaaccacca gaccggcacc ttcaacaatg aggcaggaga
    25501 gaccggcacc ttccaccgtc aggcaggaga gatccgtccc caaaccttca ccatggacca
    25561 ttccggccgg acaaaccctg gagggaagat acgatgaacc gatggcccgt gatttcagcc
    25621 gcgagggaaa catcagggat gaccttgcgg gcgctttcaa cagcgaggga tcgatcgcat
    25681 ccggaatggg cgaagccttc gcccgtgagg gctcaatagc agattcaatg gctgccgctt
    25741 tcgcttcaga aggtgtgtct gcactgggcg agtcagctat cgatgaattc attcataccg
    25801 agatatcaga cagtgagata ggtgacgctc cgggctatga ttatgaagcc ctgcccggcg
    25861 ctgatattca taaggaagga tttgacctga aaaaggctgt gatctattca gcagttcttg
    25921 acagaaaaga gtactcacgt tgatttcctg ataattactg aacggaaggg atttgttttt
    25981 taattaaaaa ttctttatat ttgttctttg aagagttccg gtaatgccgg gattcttctt
    26041 attttttaac ctatttacga gaaattatgt cggaatttgt ttatgtaacg gaagaagggc
    26101 tccagaaaat gaaagaagag cttgacctgc tccgtaatga aaggccggct attgccagaa
    26161 tgattgccga tgcgcgcgat aagggcgacc ttacagagaa cgcagaatat tctgctgcca
    26221 aggaggcaca gggtatgctt gagatgaaga ttgccaggct cgaagacatg gtagccaggt
    26281 cacgtattat tgacggctcc aggataaaca ccaaaacggt gcagatcctc aataaggtca
    26341 ggatcctcaa taaggcaaac ggttcactga tggaatatca gcttgtgcct gaaggcgaag
    26401 ccaacctcaa ggaggggaaa atcgctgcca catcacccat cgctcaggga ttgatgggca
    26461 gaaaagtggg tgacgtggta caggttaagg taccggcagg caccatcccg tttgagatta
    26521 ttgaaatatc aatctgaaaa caagatatta tggcatcaat ctttacacgc atcatcaacg
    26581 gcgagatacc ctgttacagg gtggcagaga atgaccgcta tattgcattc cttgacgtga
    26641 ggccgcttaa gaccggccac acactggtgg ttcctaaaac ggaagtggat tatatctttg
    26701 atatggatga tgaggcactc tccggaatga tgatctttgc cgggaaagtc gcccgagcaa
    26761 tgaaggaggt gatagaatgc aaaaggattg gtgtggcagt gatcggactc gaagtacccc
    26821 atgcccatat tcaccttatt cccatctccc gtgagggcga tatgctcttt acaaacccca
    26881 gggtttccct gtcaccggaa gaaaacgagc gactggcaaa agagattggt gcccgtgtaa
    26941 agcgctgatc tcagcgaaga caacaataaa attggcacca gaaaggtccg gttaaaaaaa
    27001 aagcccctgt cactgaccgg ggctttttta taatctgcag tttagttatt tcttacctgc
    27061 gcagcatgga gcggtagccg tggtgccttc ttttgccgga accacttcac cctttttgtc
    27121 gccgcattct gcagctgacg ggcactttga gcagtctttg cctttcacag cttctttctt
    27181 gcatgcatct gcggttgaac cgcaaacatc acacttgctg tccttgttct tatcgacaaa
    27241 ggtacactca ctcttggtgt gcttcttttc cttgtcggta gtctgagcat agacccctgc
    27301 agccacaaac atcatcgcga tgagaatcag gctgccaatc atccatttct tcatacttgt
    27361 gtcaattggt ttgtttatta aacggctgca atatagaaaa atttcacata ctgcttaata
    27421 gatgttacaa aaatcatacc tttggttgta cttctcgctg cgaagtgttt catattaaag
    27481 atattgccga cgacttcctg agtctgttcc tcccgcggcc atgcctgtcg tgcggtgttc
    27541 cgctggtcag gggagaagag gtgctctgta ccggctgcat gcttgacatg gcacgcactg
    27601 actttcacct ccgccgtggc aacatgctcg aacaggcttt ctggggcagg tgcagtgtgg
    27661 aaagggcagc ggcttattcg gtgtataacc gcggaagccg cataagaaag cttattcacc
    27721 tgcttaagta ctcaggcagg aaggatatcg gcatcatgct cggcaggctg tacggctctg
    27781 tcctgacggg aagcggattc actgacggga tggatatgat catccccgtg ccgctcgatc
    27841 cggccaggga aaggcgccgg ggatacaacc agagcctgtg catcgccgag ggaatggctt
    27901 cacactgccg cctgccggtg aggagcgaca ttctttgccg gacaggcaag tcaggctcac
    27961 agacaaaaag ggggaggtat gagaggtggg agaatgtcga gggactcttc acggtgacca
    28021 ggcccctgga tattgccggc cggcatgtac tgctggttga tgatgtaatc accaccggtt
    28081 caacaatgga agcctgtgta accgcgcttc agtctgccgg tggcgtcatg gtcagtgtcg
    28141 cagcacttgc tgccgcccag aaacttactg tttgaccctg cccacggaaa cttactgttt
    28201 gatcctgtcg ctgttctttt taatgaagtc gccccagttc ctgaattccc tggctgcaac
    28261 aggcttattg ctctggtaga agtggcacag ggctgcaccc agggcatcgg tggcgtcaag
    28321 aaccatcgac tccctgtgga aggcaaggat ggtccgcagc atggaagcca cctgctcctt
    28381 gctggcctgg cccctgccgg taatcgacat cttgatcatc ctcggtgcat actcgaacac
    28441 cggcaggtca cggtacagtg ctgccgcaat ggcggcaccc tgcgccctgc ccagcttgag
    28501 catgctctgt acgttcttgc cgaagaaggg agcctcaatg gcaaactcat caggatgata
    28561 cctgtcaatc agcccgagtg tctcctcgaa gatggtccgt atcttgcgga agtgatcctc
    28621 cttcttccgg aggtcaatga tgcccagggt gatgagtgac gggctcctgc ctgatacgct
    28681 tatgaggccg taacccgtga cggtggtgcc ggggtcaata ccaaggatga tcttctcaga
    28741 ctggcccggc ttcactttct gaatgcttgc ggtggcccga ggaagagctc cgtgggtaac
    28801 tggttgcctt cggaccatat gcgggggcgc ctcaccggca cggcaggggc tgcttttgtc
    28861 ccgttaagta cgccaatggc tgccttgaga gatgcctctt ccggatcacc gaaatcatgt
    28921 gttatatcat cagcagccct cacatcaggc gggatgccgt catagaaccg tccctggtcc
    28981 atcgcattca gatattcgaa ggtgatcggg gcaaagacat acttgtaatc cggttccggc
    29041 tcagcgtctg aggggaacgg atatccccag atgttcatcc cggccggttt gccgtgggtg
    29101 gtgtcaccca ctatcttcac atcgacatat ggcagaaggc tgttgatcac aacctcgctc
    29161 gcagaggctg aactgcgggt ggtgatgaaa accacgcggt caagtcccag cggactctcc
    29221 gtccggatga acttgtaact ctcattccac tcgggaccga caagtgtgtt gtatgtcagt
    29281 ttgtatgcca ccttggtggt gtcagtccat gccagcacaa ggctggcgag ctgctgggct
    29341 atatccatgt atcctccggt attgtaccgg aggtcaatga tgaggtcagt gacgttactg
    29401 gccttgaaat cggcaaaagc atcgttaagc tcctgttcag acgggtcaat gaatgtctcg
    29461 aaacacagat aacctgtctt gccgccgggg aggtcgagga cctttgatgc cgtcaccgag
    29521 ttgattatga agctcgactt gacggagttg tatgtcacct cctggccatc aggatttatg
    29581 aacttaaacg aattggttgt tcctgcggtg ccggggccca taaggttgtt gtaggcagtg
    29641 ttattgcccg agatgaagat cggggcgatg gcagtgccgt tgatctcctt tactatccac
    29701 cctcttctta cccccttcgg ccacaggtca gagccctcgt aaaggcttac caccctgaca
    29761 ttgttcatct cgtcaagacc catcctgatt ccgtgaccta cgaagctgcc ctccattgaa
    29821 gccatgaagc tctcaaagtc tgtaacgaaa ctccacctgt ccctctgcct gtatctcagg
    29881 gcttccagga gttcctcagg gccgctgtag tcagatacct tgacggtggg catgtcggta
    29941 taccagagat agacatattt catcagatca tacaggccgt cgcgcgccct ctgttccagt
    30001 gtgggggttt tgaccggttc cgggtctttc tcgcatccga aaaggaaggg aattaccaga
    30061 aaagatataa taagtatttt tttcatcatc cttggaattt gatgtaaaag taacaaaaaa
    30121 tgtgccggtt ggttaagcca gcttcgccct gttctggtag atgacgcttc ctatgatgag
    30181 aagaagcccc agcggggtgg tataataaac gggttcgtca atgatgaagt gcagcagtat
    30241 gagtgaaatg aatggagcga aatataccag gttgcttacc ttgtcagttg tctcggagag
    30301 ttgcagtgct ttcagccaca gccagaaggt gatgcccatc tcgaagaagc cgcagtagac
    30361 tgatgctgcc acccctttaa tgctcagtgc atgcgtgaat gagccaccgg tggccataag
    30421 gatgagcata tataccgatc cgaatgcgaa gctggtaaac agttttaccg actcatcgcg
    30481 cctgtcgcgt acgttaagaa cgaaataaag tgcccagagc acgccgctga caagagcaag
    30541 catcacgccc agggtgtctg atctgcccgg tttcagagga gccccctgtg acgagatgat
    30601 atataccccc accaggctta ttgccagtgc aagaaagctg cgggccggta tgcgatgacc
    30661 aagcagcggt attgaaaaga agacaaggat gatgggccat atcatgttga taggctgtgc
    30721 cacctggcct ggcagacgac tgtatgcagt cagcagaatg aggtagtagc cgaccgggtt
    30781 taccaggccc agcagtgctg accagagcag ttccctgcca gtggtctgcc tgagaaggca
    30841 taatttaccc gtaacaaaca tgatggcaaa aaggactgcg gttgagacaa gggtggtgat
    30901 acccagcatg gtcactatgc tcagttcaga cagtgctatc ttgaaggctg tagggatggt
    30961 gccccagagc atcactgaga gggcggcata aatgtatgcc tgctggcctt ttttcatttc
    31021 tttcagctga tcattccgaa cccggtgtag ggcaccagcg cggcaggtat ggtgattcct
    31081 ccgggggtct ggttgttctc aaggagagcg gctactatgc gtggcaacgc cagtgcgctt
    31141 ccgttgaggg tgtggcacag tgttgtctgt ttggccccct tttcgcggaa gcggcacctg
    31201 agcctgttgg cctggaaagc ctcgaaattc gaggtggagc ttacctccag ccagcgctgc
    31261 tgtgctgccg accatacctc aaagtcatat gtcattgccg atgtgaatga catgtcaccg
    31321 ccgcagagcc ttacaatccg gtatggcaac tccagcttca ccaggaggcc ctccacatgg
    31381 cttaccatct cctcaagcga ttcatatgat ttgtcgggat gggcaatctg cactatctca
    31441 accttgtcaa actggtgcag cctgttcagg ccccttacat gagcgcccca cgagcctgcc
    31501 tcgcggcgga agcacggagt gtatcccatg ttcttcaccg gcataacagc tgcatcaagg
    31561 atcacgtcgc ggtatatgtt tgttattggc acctcggctg tggggatcat gtaaaggttg
    31621 tcaatgccca catggtacat ctgtccctcc ttgtcgggca gctggcctgt gccgaagccc
    31681 gagtcctcat tgaccactat cggcggttct atctcacggt atcctgcctt ttctccctcg
    31741 tcgaggaaga agtttatcag tgatctctgg agttttgctc cctttccttt gtatacggga
    31801 aacccggcac cggtgatctt gatccccagg tcaaagtcta tgatgtcata tttctttatc
    31861 aggtcccagt ggggcactgc gccttcgtac agatgaggaa tggcgccacc gctgcgtacc
    31921 actacgttat cttcggcgct ttttcccggg gctaccaggg cgtggggcag gttgggcagc
    31981 ctgaccagtt cggtttgcat ctcgcctgat accacttcca gggtgtctgc cagctgcttc
    32041 tgcttgccct tcatggcgcc gatgccctga cgggcagcgt cagcctcatc cctccgccct
    32101 tccttcatca tggcaccaat ctcacgtgac agggtgttaa gctcagcctg gatggaatca
    32161 ctctcagcct gcagctcgcg ccggcggcgg tcaagatcaa gcacatgctg tactatctct
    32221 gtggcatcaa agttcttgac tttgagccgt tcaatgacga acgacgattg ttcacgaatc
    32281 aggttgatgg ttaacataac ggaaggattt ttgatgataa gcggggtaaa attaaaacaa
    32341 aaactcctga aaatatgcgc ctcagcgtat ttttcaggag ttcctgtaag ttatcagtat
    32401 cctattcagc cacgggttta accgagacgt aagatttgtt gtcgcgcttc tttttgaact
    32461 caacggttcc gtcgacgagg gcaaagaggg tatgatcctt gccgagcccg acgttttcac
    32521 cgggattatg ggtggtgcca cgctggcgaa cgattatgct gcctgccttt actgtctgtc
    32581 ctccgaagag cttgacacca agccttttgc tttctgaatc gcggccgtta cgtgaactgc
    32641 ctacaccttt cttgtgtgcc atatcgtttt ctttttactg tttatcggtt attcctt
//
LOCUS       DAOPXU010000023        32388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_1297_length_32388_cov_11.301457, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000023 DAOPXU010000000
VERSION     DAOPXU010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 32388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32388
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1297_length_32388_cov_11.301457"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..1512)
                     /locus_tag="PKK03_03790"
     CDS             complement(217..1512)
                     /locus_tag="PKK03_03790"
                     /inference="COORDINATES: protein motif:HMM:NF017396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HNX83548.1"
                     /translation="MFRLNLFVLMVAAILATGCSDKIMTLEERLASLPADTLFRIKAD
                     TSFSEAWEIRLRQPVDHRNPDGPRFTQQIFLYYAGADRPVVVETEGYGARGGRNELSS
                     ILHANLIRIEHRYFEESTPDSVDWKYLTTWQAATDHHRIIELFKPVFSGKWATTGISK
                     GGQTVMFHRYYYPDDVDVSVPYVAPLNFGPEDPRMEPFLENVGTAECRDRIFRFQKLV
                     LEKYDTLFPMFIDMADKRGWTFTRVGGPETAYEMTVLEYEFAFWQWGRISCDSIPLEG
                     NNEQVFRHLTAVGDPRYFADQGLAYFEPFFYQAMTEIGYYGYDFDKFGDLMKHAHNAE
                     KPEFQFSAPLGPEYVYDYEFGKKVSEFIKHDAENFIFLYGENDPWSASAADPGENKKC
                     LKIVNKGGAHGTRIGILPDEQKDLIMTKLQEWMDVPADL"
     gene            complement(1526..3343)
                     /locus_tag="PKK03_03795"
     CDS             complement(1526..3343)
                     /locus_tag="PKK03_03795"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HNX83549.1"
                     /translation="MAASVYMKRLAAVRALMRKKKLDALIIPSSDPHLGEYVPDHWRV
                     IRWLTGFTGSAGNVVITRTFAGLWTDSRYFIQAEEQLAGSGFELVRLKIPHTPEHIDW
                     LKEKARKGWKVGVDGRLISAVIMKLLVSSLNVSGARPDLKADLITPVWKDRPELPGDS
                     AFELDVKYAGLTRLEKLEKVREVMKVMKVDYQLLTATDDIMWLLNLRGNDVKYCPLLL
                     SFAVVSLSQVIFFADEEKIPHAVKASLDRDGVVLLPYDTVTSVLSHLDEGSVVLLSPP
                     ATPAALYRSIPRSVNITEDLSIPTRMKAVKNDVELKNLRKVMVDDGVVLTRFFHWLEK
                     AVGREAITELSAAAKIDEMRSAQAGCTGPAFSTIAAYNEHAALPHYVPDRKTDVRLKK
                     KGIFLLDSGGQYYGGTTDITRTVALGKPDAQMRREFTLALRGTIDLAMARFPRGTRGY
                     QLDILARKPLWDNYMTYGHGTGHGVGSFLNVHEGPQTIGSGATGDMKTSMEPGMVTSD
                     EPAFYRPGLYGFRTENLLVCRSDKESEFGSFLSFETVTLCYIDSELIDISVLDDRELQ
                     WLNGYHERVYQSLHRHLEPELQAWLRARTMPLSRKKKKI"
     gene            complement(3362..5515)
                     /locus_tag="PKK03_03800"
     CDS             complement(3362..5515)
                     /locus_tag="PKK03_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNX83550.1"
                     /translation="MKQFCIFLQITLLVAISASAAGQRSGAGDSQPTLVKWIDDTHFL
                     MRSMGEDRKPVYSQYDCKTGKSVSVTDYRSEMEKLRDLLPEGIQPGPDMVSSSDMQAV
                     VIVRDNDLWYYSSRIREGKKLTSDPDREMNARFAPGDRKLAYTKNKELYVYDLDLGRE
                     TRLTFDATEKIYNGWASWVYMEEILGRGTRYAAFWWSPDAGKLAYLHTDDNPVPLYYL
                     NALENTDGVHGRLEITPYPKPGDPNPKVKMGVVDLTTGKTTWVRTDENTDQYIAWPSW
                     TPDSKMLMVQVLNRDQNDMRFILADVNSGDFTEVYHETRPTWIDFFEDIYVMKDGSGF
                     IVRSCRNDWYNLYYYGWDGNLKSQITNFQWKVTDIVRVDEAKKEIYFNGTGPDLLGGH
                     LFRARLDGSKLLQVTSGEGHHTASVSPAGSYFFETWSSISDPGGINLVDKKGKVVRSI
                     HREKVPVYDASEQQKVELVKIRTSDGLFDMPALITYPVNFDPAKKYPVVFTIYGGPDA
                     GSIRNRWQGVQPRWYAENGIVTINVDHRGSGVFGKKGLDYMHRSLGKWEISDYSDAVK
                     WLRQQPWADGSRMGITGGSYGGYVTCMALTAGADYWTHGIADYSVTDWRLYDNVYTER
                     FMDTPEDNPEGYKQGSALTYAESLKGKLLIRHGDMDDNVHLQNSIWLITTLQDLNKPF
                     EMMIYPGERHGWGGPKRTFQTNETNGFWMKSFFGK"
     gene            5656..6960
                     /locus_tag="PKK03_03805"
     CDS             5656..6960
                     /locus_tag="PKK03_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83551.1"
                     /translation="MGPKGEISGRGEWMKLDNAAKIYPAITGSELTGVFRISVHLTAP
                     VVLPALLKASEEASGMFPLFSVELCRGFFWYFLEYNGKAPRVMPDSGPRCQPFPRTMN
                     GELLYRVLVRGNTISVEFFHILTDGGGAIFFLKTLLNHYFLHAEGISEMPFPEVKDGP
                     RPGCSEDLFSRHYGKGMPRPAVLPRAWHLPFRLREAPRFRVTEFGVPADRLVLLAREA
                     GSTVTEYLAATMLFVLQEIRISEGNGSPHIRVQVPFDLRRRYEAETLRNFSLFAMPAI
                     DVRMGHYSFDEILREVKITMQLMTDEKRIRQVISRNVSKEKNPLIRIIPLFIKSPVLR
                     IAYNRFGPSQFTSIVTNMGRVTPTGPAERLMQAMSVTPPPPHKDIKITAGLISLGAET
                     IITFGSLTGNTDLEKGFVKSLTSAGLPVRILTKLQQKSHGNM"
     gene            6947..7630
                     /locus_tag="PKK03_03810"
     CDS             6947..7630
                     /locus_tag="PKK03_03810"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HNX83552.1"
                     /translation="MAICKNCGVDLGEGNEKCPLCEPSESRSGKIVTAADLFRLSRIE
                     NTRHLYEITMLLLVAGVIITLAIDAVFTRGINWSLITTTCIGYLLAFLSAIYLLRRRP
                     YLVITLATVATLVFLWLLDQLTGHTGWFRSIASPLTVAAALLTAAVLFLNSLSRYRGL
                     NLLATILIALALFALVTECLTDRLLSSAYRPQWSVITAASLVIIAMFFIFIHYRMKRG
                     RSLGRLFHI"
     gene            7754..8986
                     /gene="pepT"
                     /locus_tag="PKK03_03815"
     CDS             7754..8986
                     /gene="pepT"
                     /locus_tag="PKK03_03815"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HNX83553.1"
                     /translation="MQSLLERFLGYVRFETRPDDSNPSSPSSVSQMIFAERLADELEK
                     TGLSEVTLDGNGYVMATLPANNGSRGTVTGFIAHMDTSPEAPSANINPLVHPGYDGND
                     LMLNPERRIILSPAEFPELKKYVGQTLITTDGTTLLGADDKAGIAEIVTACEYLIANP
                     SLKHGKIRIAFTPDEEIGRGADNFDVKRFGADYAYTLDGGGIGELEYENFNAAAATVT
                     VKGRSVHPGTAKDTMINSVMVAGVFDSLLPTNERPEHTAGYEGFFHLYDIRGSVEETV
                     MKYIVRDHDPVKFAARKKMLDNVAEATHKRYPGAEVNIVMRDQYFNMKEIIEANYHII
                     EAAIEAYRQAGIEPVIKPVRGGTDGARLSFMGLPCPNLFTGGHNYHGRFEYIPLQSME
                     KAVEVIINLITSRNKSPE"
     gene            8983..10611
                     /locus_tag="PKK03_03820"
     CDS             8983..10611
                     /locus_tag="PKK03_03820"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNX83554.1"
                     /translation="MKTIILPVALIALVAASCAGDSPLGKASETITPAELREYTRILG
                     ADSMMGRKPFTPGETIAVNYLARELERIGFAPAFGDSWFQEVPMVEITTKVQSPVLIT
                     AGGRKLELSAPDDIAVESPSMKEYVELVDIPMVFCGFGIVAPEYGWNDFSAVDVRGKC
                     AVVLVNDPGLYTGDSALFKGRTMTYYGRWTYKYEEAARQGATAILIIHETVGAGYDFS
                     IPRKSSISPGLYQEPVAGDPEPCPVTGWLSAEAASVLFGAQGHDVEQLRSDACIRGFS
                     GFEMGASISLRLINTHLRNSSMNVAGILRGTTRPEEAVVISAHWDHFGIGEKENGDSI
                     YNGAVDNGTSMAWALEIGEAFSSMKKRPERSVILFFPTAEEQGLLGSSWFVANPPVRQ
                     ENLIACFNNDLLLPIGRMKDMMVTGYGQSDLDDMLAGAASRQGRYLMPDPNPETGMYF
                     RSDHFPFAKAGIPSMFARGNCDSREYGIEWAADREKEYITNKYHKPADNYYPEMNFDG
                     IEEDARAVFNVACRIVTSDIRPQWKPGSEFAIVR"
     gene            10757..11101
                     /locus_tag="PKK03_03825"
     CDS             10757..11101
                     /locus_tag="PKK03_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HNX83555.1"
                     /translation="MKVEEICSVLNGELVCGSDKSQREVTHAFASDLMSDVLTVTCSN
                     LLLITGLANLQTIRTAEMSDIDCILFVRNKSVTPEMKTLAEELGMALITCPSTMFRAS
                     GELYRAGMKPLF"
     gene            11114..11539
                     /locus_tag="PKK03_03830"
     CDS             11114..11539
                     /locus_tag="PKK03_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX83556.1"
                     /translation="MSFEYRVTGGDFTHAGYASSQVKKMLKQLGVDSIVIKRVVVCLY
                     EAEVNIVAHAWDGIINVELDTDTIFMKLTDNGPGIADIEQAMQKGFSTASPEVREMGF
                     GAGMGLPNISANADRFLISSEPGVGTTLQITINLNSPAV"
     gene            11545..12939
                     /locus_tag="PKK03_03835"
     CDS             11545..12939
                     /locus_tag="PKK03_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNX83557.1"
                     /translation="MEKAFFHHALTIREDICTGCSLCMRVCPTEALRIREGKARLIED
                     RCVDCGECFRACPVHAIIIEQDDFSRIFDYKHRVALVPAVLHGQFRHEVSMSEISAVL
                     KSLGFTWVYEVEHGAEILKPAIDELMNVRTLPRPLISSYCPAVVRLIQVRFPALIRNL
                     ATVKAPLEMAAFYCRRVLRDRGAHNAEIGVFYVTPCAAKIAAVKSPVGEESTPISGVI
                     NMDFIYNKINRTLRNKGELPHSDDHAYSVDPDTLVWSLTNGEASCVNGRTLAIDGMSN
                     VTEFLEQLENDEISGVDYLELRACDEGCAGGVLLSSNRFLVAERLRNKARNLQPGNRP
                     AEDREIPDIEAYTAYLMNKVRITDEIKPRSMYRLSGDRDEAMRMMKRSRRLMCWLPGT
                     DCGLCGAPSCQALAEDIVRHRGDISQCVFIRESNLEKELMDPERAAGIMRRIWGDNNF
                     EKNCNKKGAENESN"
     gene            12926..13258
                     /locus_tag="PKK03_03840"
     CDS             12926..13258
                     /locus_tag="PKK03_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HNX83558.1"
                     /translation="MKVTDIVEKLNLKVFSGHEGLGAEITGGYASDLLSDVMGHAEAG
                     AVWVTIQTHRNVMAIASLRDLSAVIITCGEKPGDDTMTQSNEEAIPVLGTDLSTFELA
                     GRLHAILR"
     gene            13261..13977
                     /locus_tag="PKK03_03845"
     CDS             13261..13977
                     /locus_tag="PKK03_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HNX83559.1"
                     /translation="MRQIRADLHIHTLLSPCGSLEMTPREIIARAKARGLDMIAISDH
                     NSTLHCELTVALGAKAGITVLRAAEVTSAEDVHSLVILPDEAARASFQQWLDSRSEKI
                     PHNPDIFGDQVVIDENENIIAEIKYFLTAALKASIDEVEAAAHRHGALFIPAHIDRPS
                     MSITSQLGFIPDDLYIDAVEVVGRTPEILYPVIRNSDAHIPEHIGRRYTDYMMESPSF
                     GELAMAMRGESGRHIILADA"
     gene            13974..14540
                     /locus_tag="PKK03_03850"
     CDS             13974..14540
                     /locus_tag="PKK03_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX83560.1"
                     /translation="MKEIGLHIMDIVQNSIRAGAGEITVTLNESVTDDTLILTVADNG
                     KGMDEETLRKATDPWFTSRTTRKVGLGLPLLQMNASLSGGGVTIDSSPGTGTTVTASF
                     RYTHVDRPPLGDVSGTIALMILSNPSINIVYSHICEGTVWSISTSEIKEALGEEAVTD
                     LTIVRQIREIINENVAEIRNFQPGYDKY"
     gene            14617..15009
                     /locus_tag="PKK03_03855"
     CDS             14617..15009
                     /locus_tag="PKK03_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNX83561.1"
                     /translation="MEKLKSLADLKRIREEAKVKLELRVRGDNPEGLVQVKVAMATCG
                     IASGAKPIMDFFVDELDKRGIGAVVTQTGCMGYCYAEPTVEVQLPGQEPVVFGYVDRK
                     KADEIIEKYIKNGELVEGIIPVNYEKID"
     gene            15030..16823
                     /locus_tag="PKK03_03860"
     CDS             15030..16823
                     /locus_tag="PKK03_03860"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNX83562.1"
                     /translation="MSKFRMHLLVCGGTGCRASESNLILESLKRELEEKGLGNEVQVI
                     MTGCFGFCEKGPVVKVMPDNTFYVQVKPEDAAVIVAEHVIKGRPVQRLLYVDPKSKEH
                     VPDSKHMGFYRKQIRIALRNCGFINPENIDEYIAREGYAALGKCISELTPADVIDSIK
                     KSGLRGRGGAGFPTGLKWEIASKNKADQKYVVCNADEGDPGAFMDRSVLEGDPHSILE
                     AMAICGYAIGASKGLIYIRAEYPLAIQRLKIALGQAREYGLLGKNIFGSDFSFDIELR
                     YGAGAFVCGEETALIHSMEGMRGEPTNKPPFPAESGFLGKPSNVNNVETFASVPVIIS
                     KGWEWFSSIGTEKSKGTKVFALAGKINNVGLIEVPMGTTLREIIFEIGGGIKNGKKFK
                     AVQTGGPSGGCLTEKHLDTPIDFDNLVAAGSMMGSGGMIVMDEDDCMVSIAKFFLEFT
                     VEESCGKCAPCRIGNKRLHELLTHITKGNGTMADLDRLKNMCNVIKDTSLCGLGQTSP
                     NPVLSTLNNFMDEYVAHVTEKRCPAGSCRELMKYSIIVDKCVGCTACARGCPVNAISG
                     ERKGPHFIDQEKCIKCGACFEKCKFGAVLIN"
     gene            16841..18619
                     /locus_tag="PKK03_03865"
     CDS             16841..18619
                     /locus_tag="PKK03_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HNX83563.1"
                     /translation="METIKITIDNKQVEVEKGTTILDAARKMGIEIPTLCHMRLDDLN
                     IENKPGGCRICVVEVQGRKNLSPACITKCDNGSVIRTHTPRVMNARRTVMEFILSDHP
                     KDCLICPKSGTCDLQNMAHRLGIREIPGQEIAEMSTYRKDTSPSIIRELDKCIMCRRC
                     EMMCNDVQTVGAIGAFNRGFMAAVGPAYEQNLEYSPCTYCGQCVAVCPTGALTEVDHT
                     RDVLRAIVDPAKTVVVQTAPAVRAALGEEFGMKPGTLVTGKLVTALRELGFDYVFDTD
                     FAADLTIMEEGTEFLTRLKKFLNGDKNANIPILTSCCPGWVNFFEHNYPDLKDVPSTA
                     KSPQQMFGAIAKSYFADKININRKDMVVVSIMPCLAKKYESQRPEFSVDGNPDVDFSI
                     STRELANFIRQANIDFVNLPESDFDRPLGESTGAAVIFGNTGGVIEAAVRTAYELYTG
                     KKLNRLDFTELRGMEGVRQATIDFDGFPLNIGIAHGLGNARKLLDEVKAGRSNFHAIE
                     VMACPGGCIGGGGQPLHHGDSSILKARAMAIYEEDRNKPIRVSHENPYILQLYKEYLG
                     EPNSEKAHHLLHTHYFDRKAKIVVKQ"
     gene            18652..19134
                     /gene="nuoE"
                     /locus_tag="PKK03_03870"
     CDS             18652..19134
                     /gene="nuoE"
                     /locus_tag="PKK03_03870"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNX83564.1"
                     /translation="MSSIKIELRQEDLNKIHEICDSFNNEPLELINVLHRTQEHFGYL
                     PAEVQEAIADKLNISTAQVYGVVTFYSFFTMTPKGKHPISICMGTACYVRGAEKVLDE
                     FKKELALQVGQTTPDGKFSLSSLRCVGACGLAPVVLVGDKTYGRVAPDDVKAILKEYE
                     "
     gene            complement(19246..21018)
                     /locus_tag="PKK03_03875"
     CDS             complement(19246..21018)
                     /locus_tag="PKK03_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNX83565.1"
                     /translation="MKNRENSAMIWFPTSKKEMEAAGWERPDVILFTGDAYIDHPSFG
                     AAVIARVLESAGMKVAVVPQPNWRDDLRDFTKLGEPRYFFAVTAGNMDSMVNHYTAAR
                     RLRSDDAYTPGGMAGFRPDYATVVYTQILKKLFPSSPVLIGGIEASMRRLMHYDYWSD
                     RLEPSILISSGADLLIFGMAERAVAQLAGRLLAGEKMKEIHDLPQSAFTVSSGTVMPP
                     LPGIRDRALRPCHEALASRKVYAENFVLFEKTSNSMKPVRLTEQCGDVTVVVNPPLPP
                     ATEAETDAVYDLPFNYAPHPRYEKKGAIPAWEMIKFSVNIHRGCFGGCSFCAIAAHQG
                     KFISSRSEASVMREVEKISRMDGFKGYLSDLGGPSANMYRMAGRDRERCMKCGRPSCI
                     WPSVCDNLETSHRELTLLYKKVNSLPYIRKAFIGSGVRYDLLMKDYNKAAGNEEEQYL
                     RELVVNHTSGRLKVAPEHTDDEVLYLMRKSSFRHFRKLRERFMSIIRAGGLRYELIPY
                     FISAHPGTSMKKMEALARELSDLGMHPEQVQDFTPTPMTLSTAMFYLGFDPYTGKKVF
                     VERDIAGKRKQKGYFFKGKIGNRQ"
     gene            21157..23388
                     /locus_tag="PKK03_03880"
     CDS             21157..23388
                     /locus_tag="PKK03_03880"
                     /inference="COORDINATES: protein motif:HMM:NF039873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNX83566.1"
                     /translation="MIIALCGNPAPLPAQQNNARPSVGLALSGGGAKGLAHLGVMKVM
                     EEAGLKPDYVTGVSMGSIVGGMYAMGYTPDSIARMFRMFDWEYAMSDMIPEDKIIFLE
                     KRHFHNSLIALPITREAIKIPSGLISGQQIESGLNHYFWPAATITDFSKLPIPFLCLA
                     TDVVTSKKVVFRSGYLPDAIRASMAIPSVFTPVRTDTAVLVDGGVVRNYAATELREMG
                     ADIVIGSYVSFRGYKEKDLGTAYGILKQIGFLSSLADYEEQKRQTDIMIEPELGEVGT
                     LSFNNVDTIISRGYREALKYRDVFKRLADSLDAICPREPVKALPDVRYYIFDSIMVTG
                     NEIISDDQIIGVLDVRPGENVDRDLLEERIELLYGKNWFEKVRYRIVPGEEELTLEIE
                     CIERPRAMLYGSLHYDPALSAGAVVSLSVRDLITPRSVINLDSYIGQFYRVRLSATQF
                     VDKSQKFGVEFSFFADNTRLPLIKLKNETGPMLNHNFNTSLSLSKRLSLNHLMTLTAS
                     FENQHLVPDFLTSTLITRLSYDYLRLNYSYQANTLDRKHFPQQGITSGISLSALRMIR
                     GAVRTGSRSSVYFPDDEDSPFSFDRNYSARAWINSYSPVSEKVTLMLGGDVLFTTGTD
                     SVTSGNDFWLLGGTDPLSDRSITAVGFHPNQITVRNVGGLRLGTDIEITKDFHLTFDL
                     NLFALKEPYREAGFTIMGGYAIGAGYMTVAGPVRIGIMHGLYDRQVLYRAFKGYVSLG
                     FNF"
     gene            23448..25688
                     /locus_tag="PKK03_03885"
     CDS             23448..25688
                     /locus_tag="PKK03_03885"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX83567.1"
                     /translation="MNINTVILKHRSVCNLIVQRKVRQSFEIMSDMLDNVSVSDLRDH
                     FSELQMTYRNILRYTVEGIHDPERQKVYNRLLQNILRLNDRIKQDILARYSGWYTYSV
                     RAGEQKEERLRGGNIIQSVDDLVFKSRLDDLLASAEIGRTGPRSEAAMEVERLSGIIF
                     NHLWLTDYYGEAEESLMDLLIGSARFEWHEIATFVSAVTLSSLRVWDPAKIRLLARLT
                     ADLTPQVAERAVTGLVFSLHYYDDRLNLYPEVIQTVRDLAKVKNFRERCRIVVLQAIR
                     SRYTEQLSRRMNEEILPKVVKLKPRIEEKLDLDKILGENPEEGQNPDWSEMFRGSEEM
                     FKTMEELTNLQMEGSDVYMSAFAGLKKFDFFRELRNWFIPFYPDHEAVDSIFRDEILG
                     PGINELAEALYKTPFICNSDKFSLILNMKYLPSEQKSMMLKVFRMELEGLEQMKYDNE
                     LTDPAAVFRTTVTQYVQDLYRFFKLSDFRHEFDDFFTGRLDLYNSLFYRETCGSVTDD
                     RSLADYFFSKEYYEDALALYLTILKGVTDDAELYEKAGYCYQKAGEYGKALEKYSMAT
                     IIEPHTWTLRKTGWCLRRLGRPAEALEAYRRALQNEPDDLNTVLMVAHCCLDTANYDE
                     ALKHYFRVEYESPGNSKVLRPIAWCYLVTGRYTEAEKYFERLAGTGLTPYDKINMGHL
                     ALCQGSTRIAAEHYLSAMTGGEMSAESFLSAFNDDIPVLAANGVNPDDLPILLDYVLM
                     NLKKEN"
     gene            complement(25624..27657)
                     /locus_tag="PKK03_03890"
     CDS             complement(25624..27657)
                     /locus_tag="PKK03_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNX83568.1"
                     /translation="MEEKMRFFSWWRTASLLLLAVLLMPASLKSQTQTPVPVPVTLSG
                     EKIVVEGRVYYMHQVMKGQTLYSIARAYSVSVDMVARENGLVDNAIKDGQVLRIPAAA
                     VDQGQAAMKNHPAGTQTGTAGQTGQAQQAAQSGQPGQGKPAVAGAGAQVPQKQSSQDD
                     RFIYHMVRRGETLGTIANDYGITVRDLKRANRGLLFPHEGDYLLIPKNKASYRSQDQR
                     AGREQVPAGIPADTAIADTMAVIDEQETFTMPGEKNRIDRLDGTVRVAVLFPFFVREN
                     TARAYADTTERVRTGSQIYDGSLPFLEAYEGVLIAADSLRSLGLTVEMKVYDTGADSA
                     EIKRLLRTGELDDVDLIIGPVFSYNLQQIAGWAAERDIPVVSPVPLRDSHIHENKPSL
                     YRVFPSQEVVQDLIADEVASRQGSRVIFLYSDTAMYDPATASLWQKVKQATGDSAISG
                     GGRVVPYFYTGVTEKRNVYSTVASFESQLDHARKNIIVLATTSTPVVSSAFSALHSLT
                     KRYDIMVIGYPEIRGLETVDLKYYYDLELHIPSESYVDYEAPGVKSFSAMYMKKFRTE
                     PMAESFAWRGFDIAWYFIGGIASGGKSFLKDPGTFNPELLCLEPDFSRSSRNNGYENR
                     GMFILHYRKDMTIEVLRPRHQPLKGGNDQGEEVSSPFSGSSERNRAGWAGRQD"
     gene            complement(27703..28653)
                     /locus_tag="PKK03_03895"
     CDS             complement(27703..28653)
                     /locus_tag="PKK03_03895"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNX83569.1"
                     /translation="MAKSITGTDFNFPRQKGVYRGKVRDVYNIDDKYLLMVATDRISA
                     FDVVLPEGIPYKGQVLNQIATMFLEMVSDIVPVWNIASPDPNVTVGVRCQTYPVEMIV
                     RGYLTGSSWRLYKAGGREICGIPLPEGMREHQKFAKPLITPTTKAEQGMHDQDISAEA
                     IISSGLVSEEEYRKLEDYSMKLFMRGSEIAAKQGLILVDTKYEFGKRDGKIYLIDEIH
                     TPDSSRYFYSDGYEDRFARGEQQRQLSKEFVREWLMANGFQGRSGEKVPQMTPEIVRE
                     ISDRYIELYEHITGREFVKADTEDPLGRIERNVNSYLKQL"
     gene            complement(28665..29636)
                     /locus_tag="PKK03_03900"
     CDS             complement(28665..29636)
                     /locus_tag="PKK03_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNX83570.1"
                     /translation="MTEQVILLDGIDPMVMLGPGNRLLDQIQGYFPKVRLIARGNEIK
                     CVGEAEEVSVFVSRMNELIDYYRRYNRLDEGDLERLILDDEHMRSASSGDFEDALVFG
                     TSGRPIRARTVNQLQLVKEYSGNDLIIAEGPAGTGKTYTAIALAVRALKNHEVKKIVL
                     TRPAVEAGERLGFLPGDMKDKLDPYLQPLYDALHDMIPFRKLEQWIEDGTVQIAPLAF
                     MRGRTLESCFVILDEAQNATMSQLKMFLTRMGVSSKFILTGDTTQVDLPKRSESGLLR
                     AMHLLEGIEGISIIRFDERDIVRHRLVKHIVRAYDSDEKNRKEESQQ"
     gene            29772..30566
                     /locus_tag="PKK03_03905"
     CDS             29772..30566
                     /locus_tag="PKK03_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNX83571.1"
                     /translation="MPLITLTTEWREDDFFNGILRGKLASACPGAVIVNNAGGIPPLN
                     IMHGAFVIRNTFSHYPEGSIHLVFVSSEGPAEHPHLLVKAHNHWFVGANNGMFNLILN
                     SEPDLIISLRNDAGADEITLFAVTAAAIAKGQPPESLGESISSVSEKVPLRATIDRNV
                     IIGSVIFIDSYGNAITNITREIFARVFEGRDYRILIKSNNYFTEKISRFYREEPVGEI
                     VTRFNSLDLLELSINGANLCQLFSVDTGDAVRVEARNQGRETNGLF"
     gene            30579..31367
                     /gene="mazG"
                     /locus_tag="PKK03_03910"
     CDS             30579..31367
                     /gene="mazG"
                     /locus_tag="PKK03_03910"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNX83572.1"
                     /translation="MREKTIREFTRLLDIMDELRTKCPWDMKQTNQTLRKLTIEETYE
                     LADAILQDDNDAIKKELGDMMLHVTFYAKIGSEKGLFDMGDVLESINNKLVYRHPHVF
                     GDVNVTRGAREVEENWEKLKMTEHAEYKPVLSGVPASLPATVKANRIQEKVRGVGFDW
                     DSREQIWDKVTEELDELKQEIASGRKVDIESELGDLFFSLINAARLYEIDPESALEKT
                     NRRFMKRFNYLEQKTLAKGLSLRDLTLDEMNEIWEEAKQFDKPL"
     gene            complement(31364..32227)
                     /gene="speB"
                     /locus_tag="PKK03_03915"
     CDS             complement(31364..32227)
                     /gene="speB"
                     /locus_tag="PKK03_03915"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNX83573.1"
                     /translation="MAVTNFGGEEVIYSYDESKIIIVPVPYDATSTYIRGADRGPAAI
                     LEASPALEFYDIETGTEVHRYGIHTLAPLLTGDDPEEVTGEVYRTVSRLFRENRFPVV
                     IGGNHTVSIGAFRAASEAFTDLAILQLDAHSDLRPSYEGSHLNHACAAARAKEHAPLV
                     QVGIRSMSAEELPFADRKSIFFAHELYSDKTLYHKALDLLPGNVYITVDLDVFDPSVM
                     PSTGTPEPGGPDYRELMFFLRDVFSKRNVVGFDVVELCPSESNKAPDFMAAKVIYQML
                     SYRFSAQLFSS"
BASE COUNT         8158 a         8553 c         8139 g         7538 t
ORIGIN      
        1 cagaactcag cttggctaca aaaatgttca tttgaaaata aattaaactg ttaattaaga
       61 tttaaattaa ctgactgaaa atgagggaga cactaaacca tctccacaca ataaaaagtc
      121 agctgacaca aaggtattta aataatttta acaattccaa atttatttga aaaattgaag
      181 ttaacaggct gaaataaaat ctgctaaaac aatcggtcag aggtctgcag gaacgtccat
      241 ccattcctga agtttagtca tgatcaggtc cttctgttca tcaggaagga taccgatgcg
      301 ggtaccgtgt gcgcctccct tgtttacgat cttcaggcat tttttgtttt ctcccggatc
      361 agccgctgaa gctgaccagg gatcattttc gccgtaaagg aatatgaaat tttcagcatc
      421 atgtttaatg aactcactga cttttttccc gaattcataa tcatacacgt actcaggccc
      481 caggggagca gaaaactgaa attcaggttt ttccgcgtta tgagcatgct tcataagatc
      541 cccgaacttg tcaaagtcat aaccgtaata gcctatctca gtcatagcct ggtaaaagaa
      601 gggttcgaaa tatgccagcc cctgatcagc aaaatatcgg ggatcgccca ctgctgtcag
      661 gtgcctgaaa acctgttcat tgttgccttc aagtggtatg gagtcacagg aaatccttcc
      721 ccattgccag aacgcaaact catattcaag cactgtcatc tcatatgcgg tttcgggccc
      781 tcccaccctc gtgaatgtcc atccgcgctt gtcagccatg tcaatgaaca ttgggaaaag
      841 ggtatcatat ttttcaagga ccagtttctg gaaccggaag atccggtcac gacattcagc
      901 tgtgcccacg ttctcaagga acggctccat cctcgggtct tcgggcccga agttgagcgg
      961 tgccacgtat gggacactta catccacatc atcagggtaa tagtagcggt ggaacatcac
     1021 ggtctgtcct cccttgctta ttccggtggt tgcccacttg ccactgaaga cgggtttaaa
     1081 cagctcaata atccggtgat gatcagtggc tgcctgccag gtggtcagat atttccagtc
     1141 gactgaatct ggtgtcgact cctcgaaata cctgtgctca atcctgatca ggttggcatg
     1201 caggatggaa gaaagctcat tccggcctcc cctggcgcca tatccctcgg tctcaaccac
     1261 cacgggcctg tcagcgccgg cataataaag aaatatctgc tgggtaaacc ggggaccgtc
     1321 aggattacgg tggtcaaccg gttgcctgag gcgtatctcc catgcctcgc tgaatgaagt
     1381 gtcagccttt atcctgaaca gtgtatctgc cgggaggctt gccagtcgct cctccagggt
     1441 catgatcttg tctgagcatc cggtggcgag gatggctgca accattaaaa caaaaaggtt
     1501 aagtctgaac atgtgaataa taattttaga ttttcttttt tttgcggctg agcggcattg
     1561 tcctggctct cagccaggcc tgcagttccg gctcaaggtg cctgtgcagt gactggtaaa
     1621 cccgttcatg gtacccgttc agccactgaa gttctctgtc atcaaggacg gagatgtcaa
     1681 taagttcact atcaatataa cagagtgtta cagtctcaaa cgacaggaaa ctgccgaatt
     1741 cgctctcctt gtcggagcgg cataccagaa ggttttcggt cctgaaacca tagagccccg
     1801 gacggtaaaa agcgggctca tctgaagtca ccatgcccgg ctccatactg gttttcatat
     1861 caccggtggc acctgagcct atcgtctgcg gtccttcatg cacgttcagg aagctcccga
     1921 caccgtggcc ggtgccatgc ccgtaagtca tgtaattatc ccacaaaggt ttgcgggcga
     1981 gaatatcaag ctggtatccc cttgtgccac gcggaaacct ggccatggca aggtcaatgg
     2041 ttcctctcag ggcaagggtg aattccctcc tcatctgagc atcgggcttt ccgagagcaa
     2101 ccgtccttgt gatatcggtg gtccccccgt aatactgtcc gcctgaatca agcaggaaaa
     2161 tacctttctt cttcagcctg acgtcggttt tcctgtcagg gacatagtgc ggaagtgctg
     2221 catgttcatt atatgcagca atggtactga aggcaggacc cgtacatcca gcctgcgccg
     2281 agcgcatctc atcaattttc gcggcagcac tcagctcggt tatcgcttcc cttccgactg
     2341 ccttttcaag ccagtgaaaa aagcgggtca ggactacacc gtcatcaacc atcacctttc
     2401 tcaggttctt cagctcaacg tcatttttta ccgctttcat ccgcgtgggg atactcaggt
     2461 cctctgtgat attcacgctg cggggaatag atctgtacag tgcagccggg gttgccgggg
     2521 gtgaaagcag aacgactgat ccctcatcca ggtgagagag gacggaggtc actgtgtcat
     2581 acggcagcag aacaacaccg tctctgtcaa gtgaggcctt tacagcatgc ggaattttct
     2641 cctcatcagc aaagaagatg acctggctga ggctcaccac agcaaaagaa agcagcagcg
     2701 gacagtactt cacatcgttg cccctgagat tcaacagcca catgatatca tctgttgctg
     2761 taagcagctg ataatcaacc ttcattacct tcatcacctc cctgactttt tcaagcttct
     2821 caagcctggt cagcccggca tactttacat caagttcaaa agcactgtca ccgggcagct
     2881 cagggcgatc cttccagaca ggagtgatca ggtcggcttt caggtcgggc ctggcaccgg
     2941 agacattaag ggatgacacc agcagcttca ttataactgc tgagatcagg cggccgtcaa
     3001 cgcctacctt ccaccccttg cgggcctttt ccttcagcca gtcgatatgc tccggggtat
     3061 gcggaatctt cagccgcaca agctcaaatc ccgaaccggc cagctgttct tcagcctgaa
     3121 tgaagtaccg gctgtcagtc cacagaccgg cgaaagttct ggtaatgaca acgttcccgg
     3181 cagagccggt gaagccggtc agccacctga taaccctcca gtgatcaggc acgtattcgc
     3241 caaggtgcgg atcggaggag ggtattatta gtgcatccag cttctttttc ctcatcaggg
     3301 cccgcactgc ggcaagcctc ttcatgtata cactcgcagc catatccctt attatgtgta
     3361 tttacttccc aaagaacgat ttcatccaga atccgttggt ctcattggtc tggaaggttc
     3421 tcttgggtcc tccccagccg tgacgttcac ccggatatat catcatctca aaaggtttgt
     3481 tgagatcctg gagcgtggtg atcagccaga tggagttctg cagatggacg ttgtcatcca
     3541 tatcgccatg gcggatgaga agctttccct tcagtgattc cgcatatgtc agggctgagc
     3601 cctgcttata cccttccgga ttgtcctccg gggtatccat gaatcgttcg gtatatacat
     3661 tgtcataaag acgccagtcg gtgactgaat agtctgcgat gccgtgagtc cagtagtctg
     3721 cgcctgccgt cagggccatg catgtgacat acccgccgta agaaccgccg gtaatgccca
     3781 tcctggaacc gtcagcccac ggttgctgcc taagccattt caccgcatct gaatagtccg
     3841 agatctccca tttccccagt gagcgatgca tgtagtcgag tcccttcttg ccgaagacac
     3901 ctgaaccccg gtgatcaaca ttgatggtta cgatcccgtt ctcggcatac cagcggggct
     3961 gaactccctg ccacctgttc ctgatgctgc cggcatccgg accgccgtaa atggtgaaca
     4021 caaccggata tttttttgcc ggatcgaaat tcaccggata agttatcagt gcaggcatgt
     4081 caaacagacc atcggatgtc ctgatcttca caagctcaac cttctgctgt tccgaagcat
     4141 catagacggg taccttttca cgatggatgc tgcgtaccac tttccccttt ttatcaacca
     4201 ggttaatacc accggggtca ctgatgctgc tccaggtttc aaagaaatat gatccggcgg
     4261 gagagaccga tgcagtgtga tgcccctctc ctgaagtgac ctgcagcagt tttgatccgt
     4321 caagccttgc acggaacaga tgaccgccaa gcagatcagg accggtgccg ttgaagtata
     4381 tttccttttt ggcttcatca accctgacga tatcagtcac tttccactgg aagttggtta
     4441 tctgcgattt aaggttacca tcccatccgt aataatacag attgtaccag tcattgcggc
     4501 aactccttac gatgaatccg ctgccgtcct tcattacata aatatcctcg aagaaatcaa
     4561 tccaggtggg cctggtttcg tggtatactt cggtaaaatc cccgctgttc acgtcggcca
     4621 ggatgaacct catgtcattc tgatcgcggt tgaggacctg aaccataagc attttgctgt
     4681 caggggtcca tgatggccat gctatatact ggtcagtatt ctcatcagtc ctgacccatg
     4741 ttgttttacc ggttgtgaga tcaaccaccc ccattttaac ctttgggtta gggtctccgg
     4801 gcttgggata aggagttatc tcgagcctgc cgtgaacgcc gtcagtattt tcaagggcat
     4861 tcagataata gagaggaacc gggttgtcat cggtatggag gtaggccagt ttacctgcgt
     4921 caggagacca ccagaatgca gcatacctcg tgcctctgcc gagaatctcc tccatataaa
     4981 cccatgatgc ccagccgttg tagatttttt cggttgcatc aaaggtgagc cttgtttccc
     5041 ttcccaggtc aagatcatat acgtaaagtt ccttgttctt cgtgtaggcc agtttgcggt
     5101 cgccgggggc aaacctggca ttcatctccc tgtcggggtc ggaagtcagc tttttccctt
     5161 cacggattct tgaagaataa taccagaggt cattgtcgcg gacaatcaca acagcctgca
     5221 tatctgagga agaaaccatg tcaggaccgg gctgaatccc ttccggaaga aggtccctga
     5281 gcttttccat ctcgctcctg tagtcagtga cagagacgga tttgccggtc ttgcagtcat
     5341 actgcgagta aaccggcttt ctgtcttcac ccatgctcct catcagaaag tgggtgtcat
     5401 caatccattt gacaagcgtc ggctgcgaat cacctgcgcc ggacctctgg cctgcggctg
     5461 aagcagaaat agccaccaac agggtgattt gtaaaaagat gcaaaactgt ttcatggtat
     5521 aggaatttga gttcgtatcg ttctcctgag ctaacaaata taataaactt aaatcccgcc
     5581 ggtgtgtaag atgccggaga acacaaatta caaacgggag catatatgtt tatctttacg
     5641 tcggaataga ggaaaatggg tccaaaagga gaaatctcag gacgtgggga atggatgaag
     5701 cttgacaacg cagccaagat ctatccggca atcacgggtt cggaactgac cggggtattc
     5761 cggataagcg tgcatcttac tgcacctgtt gtgctgcctg cgcttttaaa ggcatctgaa
     5821 gaagcttcag ggatgtttcc gctgttcagt gtggaactgt gcaggggttt cttctggtat
     5881 ttccttgagt acaatggcaa agcgccgagg gtgatgcctg acagcggtcc gcgctgccag
     5941 ccctttcctc gaaccatgaa cggtgagctt ctttaccgtg tccttgtccg tggcaatact
     6001 atctcggtgg agttctttca catcctgacc gatggtggtg gcgccatttt cttcctcaag
     6061 acactcctca accattactt ccttcatgca gaaggtattt cagagatgcc gttcccggaa
     6121 gtcaaggacg gccctcgtcc gggttgcagc gaggatctct tttccagaca ttacgggaaa
     6181 gggatgcccc ggcctgccgt tcttccccgc gcatggcacc tgccgttcag gctgcgggag
     6241 gcccccaggt tcagggtaac ggaattcggc gtaccggctg acagactggt actgctggca
     6301 agagaggcag gtagcaccgt gaccgagtac cttgcagcca caatgctctt cgtactccag
     6361 gaaataagga taagcgaagg aaacggctca ccacatatac gggtacaggt cccgttcgat
     6421 ctgcgcagga gatacgaggc agaaaccctg cgtaacttct cactcttcgc aatgccggca
     6481 atcgatgtca ggatgggcca ttactctttc gacgagatcc tgcgcgaggt gaaaatcaca
     6541 atgcagctga tgactgacga aaagagaatc agacaggtca tcagcagaaa cgtgtcaaag
     6601 gaaaagaatc ctcttatccg cattattcct ctgttcatca agagtcctgt tctgcggatt
     6661 gcctacaacc ggttcggccc gagccagttc acatcgatag tcaccaacat gggaagggta
     6721 accccgacag gccccgctga acggctgatg caggcaatgt cagtcacccc ccctccccct
     6781 cataaggata tcaagatcac cgccggactt atctcactcg gcgctgagac aattattacc
     6841 ttcggatcac tgaccggcaa caccgacctt gagaagggct ttgtgaagag tctcacctca
     6901 gccggactgc cggtaagaat cctgaccaaa ttacagcaaa aaagccatgg caatatgtaa
     6961 aaactgtggg gtggaccttg gagagggcaa tgagaaatgc ccgctctgtg aaccctctga
     7021 aagccggtcc ggaaagattg tgaccgctgc tgacctgttc aggctctcaa ggatagagaa
     7081 cacacggcac ctgtatgaga ttacaatgct cctcctggta gcaggcgtga taataacact
     7141 ggccattgat gcggtcttta ccaggggaat aaactggtca ctgataacaa ccacatgcat
     7201 aggatacttg ctggcattcc tttctgccat ttacctgctc agacgcagac cttacctcgt
     7261 catcacgctg gctaccgtgg caaccctggt attcctgtgg ctccttgacc agctcaccgg
     7321 tcatacaggc tggttccgtt ccatcgccag cccgctgaca gtggctgctg cccttcttac
     7381 agcagcagtc ctcttcctga actccctcag caggtacagg ggactgaacc tcctggcaac
     7441 aatacttatc gccctggcac tattcgccct cgtcacggag tgcctcacag accgccttct
     7501 ctcttcggcc tacaggccgc aatggtcagt gattacagca gcatcactgg tgattatcgc
     7561 aatgttcttt atcttcatcc attacaggat gaagagaggc agaagccttg gccgcctgtt
     7621 ccatatctga aatatctttg attttccggc tccctgatga aacgatacct ggtcaccgga
     7681 atgatgacgg tgaatggatg ccgtaccttc agttttaatg actaaattta acagcttaac
     7741 caaataaaca cggatgcaga gtctccttga aagatttctc gggtatgtca gatttgagac
     7801 aagacctgat gattcaaacc cctcctcacc ctcatcggta agccagatga tttttgccga
     7861 acggctggct gatgagctgg aaaagacagg gctgtcagaa gtgacactcg acggcaacgg
     7921 atacgtgatg gccacactcc cggcaaacaa cggctcccgg ggcactgtta ccggctttat
     7981 tgcacatatg gacaccagtc cggaagcccc ctcggcaaac attaatcccc tggtgcatcc
     8041 cggctatgac ggaaatgatc tgatgcttaa cccggaaaga agaatcatcc tgtcccccgc
     8101 tgagttcccg gaactgaaga aatatgtcgg acagacactg ataaccactg acggaaccac
     8161 gcttctcggg gctgatgaca aggccggtat cgctgagata gtcaccgcat gcgagtatct
     8221 cattgcaaac ccttcactca aacacggaaa gatcaggatt gcctttacac ccgatgagga
     8281 gatcggaaga ggagctgaca atttcgacgt gaaacggttc ggggctgatt atgcctatac
     8341 cctcgacgga ggaggcatag gggaactgga atatgaaaac ttcaatgctg ccgcggcaac
     8401 ggttactgta aagggacgca gcgtgcaccc cgggacggca aaggatacaa tgatcaactc
     8461 ggttatggtg gccggcgtct tcgacagcct cctgccaacg aatgagagac cggaacatac
     8521 tgccggttac gagggatttt ttcacctcta tgacatccgc ggttctgttg aagaaactgt
     8581 catgaagtac attgtcaggg atcatgaccc ggttaaattt gccgccagga agaaaatgct
     8641 ggataatgtc gcggaagcaa cacataaaag gtatccggga gctgaagtga acattgtcat
     8701 gcgcgaccag tacttcaata tgaaagaaat aattgaagcc aactatcaca tcattgaggc
     8761 tgccatcgaa gcctaccggc aggccgggat agaaccggtc atcaaacctg tcaggggcgg
     8821 aaccgacggt gccaggcttt cattcatggg cctgccatgc cccaacctct tcaccggcgg
     8881 acataactac cacggacggt ttgaatatat cccgcttcag tcaatggaaa aagccgtgga
     8941 ggtaataata aatctcatca cttcacgaaa caaatcaccg gaatgaagac aataatcctt
     9001 cctgtggctc tgatagccct ggtagcagcg tcatgcgcag gagacagtcc gctcggaaaa
     9061 gcctctgaaa ccatcacccc cgctgaactg agggaatata cccggattct gggtgctgac
     9121 agcatgatgg gacgcaaacc ctttaccccc ggtgagacaa ttgccgtaaa ctatcttgcc
     9181 cgagagctcg aaagaatagg attcgctcct gctttcggcg acagctggtt tcaggaagtt
     9241 cctatggtgg agatcaccac aaaggtgcaa agcccggttt tgatcactgc cggcggcagg
     9301 aaactggagc tctcagcacc tgatgatatc gcagtggaat cgccttcaat gaaagagtat
     9361 gttgaactgg ttgacatacc gatggtcttc tgcggcttcg gcattgtggc cccggaatac
     9421 ggctggaatg atttctcggc agttgatgtc agaggcaaat gcgcagtggt tctcgtgaat
     9481 gacccgggac tctataccgg cgactccgcc ctgttcaagg gaaggacaat gacatactac
     9541 ggaaggtgga catacaagta cgaggaggcg gccaggcagg gggcgacggc aattctgatc
     9601 atccatgaga ctgtgggcgc aggctatgac ttctcgatac cccgcaaaag ttcaatctca
     9661 cccggccttt accaggagcc tgttgccggt gatccggaac catgtccggt cacaggctgg
     9721 ctcagcgccg aagcagcatc agtgctgttc ggagcccagg gccatgatgt tgagcagctg
     9781 cgaagtgatg cctgcatcag aggcttcagc ggctttgaaa tgggcgcgtc aatctcactg
     9841 cgcctcatca acacccacct caggaacagc tcaatgaacg tggcaggaat cctcaggggc
     9901 acaacccgcc ccgaagaggc tgtagtgatt tcagcccact gggatcactt tggcatcgga
     9961 gagaaagaga acggcgattc gatatacaac ggtgcggttg acaatggcac atcgatggca
    10021 tgggcccttg agatcggcga agctttctct tccatgaaaa agagaccgga aagatccgtg
    10081 atccttttct ttcccacggc agaggaacag ggattactcg gctcatcctg gtttgtggcc
    10141 aaccctcctg tcaggcagga gaacctgata gcatgcttca ataatgacct tctcctcccc
    10201 attgggagga tgaaagacat gatggtaacc ggttacggac agtcggacct ggatgacatg
    10261 ctggcaggag ccgccagccg ccagggaaga taccttatgc ccgatcccaa tcccgaaaca
    10321 ggcatgtact tccgttctga ccacttcccg ttcgccaagg caggcatccc ctcgatgttc
    10381 gcccggggca actgtgacag cagggaatac ggcattgaat gggctgccga tcgtgaaaag
    10441 gaatacataa ccaataaata ccacaaacct gccgataact actaccccga aatgaacttc
    10501 gacgggatag aggaagatgc cagggccgtg ttcaacgtag cctgcaggat cgtcacaagc
    10561 gatatcaggc ctcagtggaa gcccggctcc gagttcgcca ttgtaaggta aaagactgtc
    10621 atacagacaa taacaaacaa tgagacaata tctttaatac ttttctgtta tttttgtaac
    10681 actgtgaatt tattcacaaa taatatttat ctttgcactg ccatttataa taaagtgtat
    10741 ttgaaagact ggtaacatga aagttgaaga gatatgttcc gtattgaacg gcgagctggt
    10801 ctgcggcagt gacaaaagtc agcgtgaagt gactcacgca tttgcatctg atctgatgag
    10861 tgatgtgctg actgtaacct gcagcaatct gctgctgatc accgggcttg ccaatctgca
    10921 gaccatacgt acggccgaga tgtccgacat agactgcatc ctgtttgtaa gaaacaagtc
    10981 tgtcacaccg gagatgaaaa cgcttgccga agagctcgga atggctctga taacctgccc
    11041 ctcaacaatg ttcagggcat ccggggagct ttacagggca gggatgaaac cgttattctg
    11101 acaaaccgtg gcaatgagct tcgagtatag ggtcacggga ggcgatttca cccacgcggg
    11161 ttatgcctcc agccaggtca agaagatgct gaagcagctc ggtgtggaca gtatcgtcat
    11221 caaaagggtg gtggtatgcc tctatgaagc cgaagtgaac atcgttgcac atgcctggga
    11281 cggaataata aacgttgagc tcgataccga cactatcttc atgaaactga ctgacaacgg
    11341 tcctggcatt gccgatattg aacaggcaat gcagaaagga ttctcaactg cttctcccga
    11401 ggtgcgggag atgggattcg gcgcaggaat ggggctgcca aacatcagcg ccaacgctga
    11461 ccggttcctg atcagcagcg aaccgggagt cggcaccacc cttcagataa caattaacct
    11521 gaacagccct gctgtctgac aattatggaa aaagcattct ttcatcacgc acttacgatc
    11581 cgcgaggata tatgcaccgg atgctccctc tgcatgaggg tctgccctac tgaagcactc
    11641 cgtatccgtg aaggaaaggc aagactgata gaagacaggt gtgtcgactg cggcgagtgc
    11701 ttcagggcct gccccgtaca cgcaatcatc atcgaacagg acgacttcag ccgtattttc
    11761 gattacaaac accgcgtagc cctggtaccg gcagtgcttc acggccagtt ccgccacgaa
    11821 gtatcaatgt cagaaataag cgccgtactt aaaagcctgg gattcacatg ggtatatgag
    11881 gtggagcacg gagcggaaat actgaagccg gcaatagatg aactgatgaa cgtccggact
    11941 ctcccccgcc ctctcatatc aagctactgt cctgccgttg tacgactcat acaggtgcgc
    12001 ttccccgctc tgatcagaaa cctggcaacg gtcaaagccc cgctcgagat ggcagccttc
    12061 tactgccgca gggtgctgcg tgacagaggc gcccataatg ccgaaatcgg cgtgttctac
    12121 gtcacccctt gtgcagccaa gatagcagct gtcaaatcac ccgtcggaga agaatcgact
    12181 cccataagcg gggtgatcaa catggacttc atatacaata agataaaccg caccctcagg
    12241 aacaagggag aactgccgca cagcgatgat cacgcctatt ccgtcgaccc tgacaccctc
    12301 gtctggagcc tgaccaacgg agaggcctct tgtgtcaacg gacgcaccct tgccattgac
    12361 ggcatgagta acgtgaccga attccttgaa cagctggaga acgatgagat atcaggagtt
    12421 gattacctgg agctgagagc atgcgatgaa ggttgtgccg gaggagtgct tctctcctcc
    12481 aaccgtttcc tggtggcgga gcggctccgc aacaaggcac gtaaccttca gccgggtaac
    12541 aggcccgctg aagacaggga aatacctgac atagaagctt ataccgcata cctgatgaac
    12601 aaggtgcgca tcaccgatga gataaagcca aggtcaatgt accgtctctc gggtgaccgt
    12661 gacgaggcaa tgcgaatgat gaaacggtca cgccggctga tgtgctggct gccgggaacc
    12721 gactgcggac tttgcggtgc accttcatgc caggcactgg ctgaagacat cgtccgccac
    12781 cgcggagaca tatcacagtg cgttttcatc cgggaatcaa acctcgaaaa ggaactgatg
    12841 gaccctgaaa gggctgccgg tatcatgaga aggatctggg gcgacaataa ttttgaaaag
    12901 aactgcaata agaaaggagc tgaaaatgaa agtaactgat atcgttgaaa aactgaacct
    12961 gaaggttttc tcagggcatg aggggctcgg agctgagata acaggcggat atgcctctga
    13021 ccttctcagt gatgtcatgg gtcatgccga ggcaggtgcg gtgtgggtca caatacagac
    13081 tcaccgcaat gtgatggcga tagcctcact cagggatctc tctgccgtaa tcatcacctg
    13141 cggtgagaaa ccgggtgacg atacaatgac acagagcaac gaggaggcca tcccggtgct
    13201 gggcactgat ctgtcaacct ttgagcttgc aggcaggctt cacgcaatac tccgctgaca
    13261 atgagacaga tcagggctga cctccatatc cataccctcc tttcgccatg cggctcactg
    13321 gagatgactc cacgtgaaat aatagccagg gcaaaggcca ggggactcga tatgattgcc
    13381 atctctgatc ataacagcac cctgcactgt gaactgaccg tagctctcgg ggcaaaagcc
    13441 ggcataaccg tcctccgtgc agccgaagtg acaagcgccg aggatgttca ctcacttgta
    13501 atcctccctg acgaggcggc acgcgcttcc tttcagcagt ggctcgacag ccgcagtgag
    13561 aaaatccctc acaacccgga tatattcggc gaccaggtgg tcattgatga aaatgagaac
    13621 atcattgcag agataaagta ctttctcact gcagccctga aagcctcgat cgacgaggtt
    13681 gaagctgccg ctcacaggca cggagcactc tttatcccgg cccatattga ccgcccctcc
    13741 atgagcataa ccagccagct tggcttcatc cccgatgacc tgtacatcga tgccgtcgag
    13801 gtggtgggcc gtactccgga aatactctat cccgtcatcc gcaattcaga tgcccacatc
    13861 cctgaacaca tagggagacg ctataccgac tatatgatgg aaagcccctc attcggggaa
    13921 ctggccatgg cgatgagggg agagagcggc aggcacataa tacttgctga cgcatgaagg
    13981 agataggtct gcacattatg gacattgttc agaactccat cagggccgga gccggcgaga
    14041 tcacagttac gctgaacgaa tccgtcacag atgacacact gatacttaca gttgccgaca
    14101 acggcaaggg gatggatgag gagaccctcc gaaaggctac cgatccctgg tttacctcac
    14161 ggacaacccg taaggtggga ctgggtctgc ctctgctcca gatgaatgca agcctctccg
    14221 gtggaggggt gacaatagat tcctctcccg gcacaggtac caccgtcacc gcatccttcc
    14281 ggtacaccca tgtggacagg cccccgctgg gcgacgtgag cggaacaatt gctctgatga
    14341 ttctgtcaaa cccatccata aacatcgtgt attcccatat ctgtgaagga acggtatgga
    14401 gcatctccac cagtgagata aaggaggccc tcggtgagga ggctgtcaca gacctgacca
    14461 ttgtcaggca gatcagggaa atcataaatg agaatgtggc tgagatcaga aattttcaac
    14521 cgggatatga caaatattag gtatttagta acttattgag gttagttttg ccgatcgatc
    14581 agaagataca aattactcag aataaaaaca tttgaaatgg aaaaactcaa atcacttgca
    14641 gacttaaaga ggatccgtga ggaagcaaag gttaagcttg aactgagggt caggggagac
    14701 aatcccgaag gactcgtcca ggtcaaggtt gccatggcta cctgcgggat cgcatcaggt
    14761 gcaaagccga taatggattt ctttgttgat gagcttgata agagaggcat tggcgccgtt
    14821 gtaacccaga cgggatgcat gggctattgc tatgccgaac ctacggttga ggttcagctc
    14881 cccggccagg aacctgtcgt tttcggctac gttgacagga aaaaagctga tgagatcatt
    14941 gagaaataca tcaagaacgg tgaactggtt gaaggtatca taccggttaa ctatgaaaaa
    15001 atagactaat aacagggagg aaatatataa tgtcaaaatt cagaatgcac cttcttgtct
    15061 gtggcgggac aggctgtcgc gcctcagaaa gcaatctcat acttgagagt ctgaagaggg
    15121 aacttgaaga aaaaggcctc ggtaatgagg tacaggttat aatgaccggc tgtttcggct
    15181 tctgcgagaa gggacccgtt gtcaaggtta tgcctgacaa caccttctat gtgcaggtca
    15241 agcctgagga tgcagcagtt attgttgccg agcatgttat taaaggaaga ccggtacagc
    15301 gcctgctgta cgttgatccg aagagcaagg aacatgtgcc cgattcaaag cacatgggtt
    15361 tctaccggaa gcagatccgc atagccctgc gtaactgcgg tttcatcaac ccggaaaaca
    15421 tagatgaata catcgcacgt gaagggtatg ctgcccttgg caagtgcatc agtgaactca
    15481 cccccgccga tgtcattgac tcaataaaga aatcaggcct ccgcggacgt ggcggtgccg
    15541 gattcccgac cgggctcaag tgggagatcg cttcaaagaa caaggctgac cagaagtacg
    15601 tggtctgcaa cgctgacgag ggtgacccgg gtgcattcat ggaccgttcg gttcttgaag
    15661 gcgaccccca ctccatcctt gaggcaatgg ccatctgtgg ttatgccatc ggcgcatcaa
    15721 aaggcctcat ctacatcagg gccgaatacc cgctggcaat tcagcgtctt aaaatagccc
    15781 ttggtcaggc acgggaatac ggcctcctcg ggaaaaacat tttcggcagt gacttcagct
    15841 tcgacattga actgcgctat ggcgccggag cattcgtctg cggtgaggag acagcgctga
    15901 tccattcaat ggaaggaatg cgcggcgaac ccaccaacaa acccccgttc ccggcagaaa
    15961 gcggattcct tggcaaaccc tcaaacgtaa ataacgtcga gactttcgcc agcgtccccg
    16021 tgatcatttc caagggatgg gaatggttct catcaatcgg aactgaaaaa tccaagggaa
    16081 cgaaggtatt cgcccttgca ggcaagataa acaacgtggg gctcattgaa gtacccatgg
    16141 gcacaaccct ccgcgaaatc atcttcgaga tcggcggcgg aataaagaac ggcaaaaagt
    16201 tcaaggctgt ccagaccggc ggaccttccg gcggctgtct caccgagaaa caccttgata
    16261 caccgatcga ctttgataac ctcgtggctg caggatcaat gatgggatca ggcgggatga
    16321 tcgtcatgga cgaggatgac tgcatggttt caatcgcaaa attcttcctt gaatttactg
    16381 tggaagagtc atgcggcaag tgtgctccct gccgtattgg caacaagaga ctccatgagc
    16441 tcctcaccca tatcaccaag ggcaacggca caatggctga ccttgaccgc ctgaagaaca
    16501 tgtgcaatgt catcaaagac acttcactgt gcggacttgg ccagacctct ccgaaccctg
    16561 tactctcaac actgaacaac ttcatggatg agtatgtggc tcacgtcacc gaaaagaggt
    16621 gcccggccgg ttcatgccgt gaactcatga agtactcaat catcgttgac aaatgcgtcg
    16681 gctgtaccgc ctgcgccagg ggatgccctg tcaacgccat cagcggtgaa cgcaagggac
    16741 cgcacttcat tgaccaggag aaatgcatca aatgcggagc ctgcttcgag aaatgcaaat
    16801 ttggtgctgt gctaatcaac taataccagg aagagttacg atggaaacaa taaaaattac
    16861 aatagacaac aaacaggttg aagtcgagaa gggaactacc atcctcgatg ctgcaaggaa
    16921 gatggggatc gaaattccca ccctctgcca catgaggctt gacgacctga acattgaaaa
    16981 caaacccggc ggatgcagga tatgcgttgt ggaagtccag ggacgcaaga acctctctcc
    17041 agcatgtatt acaaaatgtg ataacggctc tgtcatacgc actcacaccc cgagggtgat
    17101 gaatgcccgc aggaccgtga tggaattcat cctctcggat cacccgaagg attgcctcat
    17161 atgcccgaag tcaggcacct gtgatctgca gaatatggct caccggcttg gcatcaggga
    17221 gatccccggc caggagattg cagagatgtc aacctacagg aaagacacct ccccgtcaat
    17281 catccgcgag cttgacaagt gcatcatgtg ccgccgttgc gagatgatgt gcaatgacgt
    17341 ccagaccgtt ggtgcaatag gagccttcaa caggggcttc atggctgccg tgggcccggc
    17401 ttacgaacag aacctagagt actcaccctg tacctattgc ggccagtgcg tggcagtgtg
    17461 ccccaccggc gccctgactg aggtggacca tacacgcgat gtgctccgcg ccatcgtcga
    17521 ccccgccaag acggtggtcg tacagacagc cccggcagtg cgcgccgccc tcggcgaaga
    17581 attcggcatg aaacccggaa ctctcgtcac cggcaaactg gtgactgcac tcagggaact
    17641 cggattcgac tatgtgtttg ataccgactt tgctgctgac ctcaccatca tggaagaggg
    17701 tacggaattc ctcaccagac taaaaaaatt cctgaacggt gacaagaacg caaatattcc
    17761 gattcttact tcatgctgcc cgggttgggt caacttcttc gaacacaact atcccgacct
    17821 gaaggacgtg ccatcaacgg ccaaatctcc gcagcagatg ttcggcgcaa ttgccaagtc
    17881 ttacttcgca gataagatca atatcaaccg taaggatatg gtggtggtct ccattatgcc
    17941 ttgcctcgcc aagaagtacg agagtcagag acctgagttt tccgtcgatg gcaacccgga
    18001 tgttgacttc tcaatctcaa cgcgtgagct ggcaaacttc atccgtcagg caaacatcga
    18061 ctttgtgaac ctccctgaat ctgactttga cagaccgctc ggcgaatcga ccggcgcagc
    18121 tgtcatcttc gggaacaccg ggggcgtcat tgaagctgcc gtccgtacag cctacgaact
    18181 ttacaccggc aagaaactca accgccttga tttcactgaa ctgagaggaa tggaaggagt
    18241 gcgccaggct accatcgatt ttgacggctt cccgctcaat atcggtatcg ctcacggcct
    18301 gggcaacgcc cgcaagctcc tcgacgaggt gaaggcaggt cgctcaaatt tccatgccat
    18361 tgaggtaatg gcatgccccg gcggttgtat cggcggaggc ggccagccac tgcatcacgg
    18421 tgactcgtca atactcaagg caagagcaat ggcaatctat gaggaagacc gtaacaagcc
    18481 aatcagggtg tctcacgaaa acccgtacat tctccagctc tacaaggagt atcttggcga
    18541 gcccaacagc gaaaaggctc accacctgct gcatacacac tatttcgaca gaaaggcgaa
    18601 gatcgtcgtc aaacagtgat accgcggtaa ttaacattaa aaatcaaaga gatgtcaagt
    18661 ataaagatag aactgagaca ggaagatctg aacaagatcc atgagatctg cgattctttc
    18721 aacaatgaac cgctggagct gataaacgtc cttcacagga cacaggaaca tttcggctac
    18781 ctgcctgccg aggtgcagga agctattgcc gataaactga atatatccac agcacaggtt
    18841 tacggcgtgg tgaccttcta ttcattcttt accatgaccc cgaagggcaa gcacccgata
    18901 agcatatgca tgggtactgc ctgttacgtc agaggtgcag aaaaggtcct cgatgagttc
    18961 aagaaagagc tggccctcca ggttggacaa acaacccctg acgggaaatt ctctctctca
    19021 agccttcgct gcgtgggcgc atgcggactg gcaccggtgg tgctcgtcgg tgacaaaacc
    19081 tacggaaggg tcgcccccga tgatgtgaaa gcgatactga aagaatacga gtagtaaaaa
    19141 agaggccggc agtaccggcc ttttttttca ggcaatagcc attggacagt aggcaggagt
    19201 ggcactttca ggataacagg cagtcagtga tgattcattg tcacactatt gcctattgcc
    19261 tattttgcct ttgaaaaagt accccttctg ctttctcttc ccggcaatgt cacgctcgac
    19321 aaagaccttt ttccccgtat agggatcaaa cccgaggtag aacatggctg tggagagtgt
    19381 catgggagtc ggggtaaaat cctgcacctg ctccggatgc atccccaggt cagagagctc
    19441 acgtgccagc gcctccattt tcttcatgct ggtgccggga tgggctgaga taaagtaagg
    19501 gatgagctca tacctgagcc cgccagccct gattattgac atgaaccttt cccggagctt
    19561 ccggaagtgc ctgaaagaac tctttctcat caggtaaagc acctcgtcat ccgtatgttc
    19621 aggggccacc ttgagcctgc cgcttgtgtg gttcaccacc agctcccgca ggtactgttc
    19681 ttcttcattg ccggcagctt tgttgtaatc cttcatgagc aggtcatatc ttaccccgct
    19741 gccgatgaat gctttcctga tatatggcag gctgttgact tttttgtaaa gcaaggtaag
    19801 ttccctgtgg gaagtctcca ggttgtcaca cacagagggc cagatgcatg acgggcgccc
    19861 gcatttcatg catctctccc tgtccctccc ggccatcctg tacatgttgg ccgaaggtcc
    19921 gcccaggtct gacagataac ctttaaatcc atccatccgg cttatcttct caacctccct
    19981 cattaccgag gcttccgaac ggctgctgat gaacttgccc tggtgggcgg cgatggcgca
    20041 gaagctgcat ccgccgaaac agccacggtg gatgttgact gagaacttta tcatctccca
    20101 tgcagggatg gcccccttct tttcatacct gggatgagga gcgtaattga acggcaggtc
    20161 ataaacagca tccgtttcag cctctgttgc gggcggaaga ggcgggttga caaccactgt
    20221 aacatcgccg cactgctcgg tgagcctgac aggcttcata ctgttgcttg tcttctcgaa
    20281 caggacaaag ttctcggcat agaccttcct ggaagccaga gcctcatgac acggcctcag
    20341 cgccctgtcc ctgattcccg gaagaggtgg catcaccgta cccgatgata ctgtgaaggc
    20401 cgactggggc agatcatgta tctccttcat cttctccccg gccagaagcc tcccggcaag
    20461 ttgtgccacc gccctttcgg ccatgccaaa aatgagcagg tcagcgccgc tggagattag
    20521 tatcgaaggc tccagcctgt cactccagta gtcataatgc atcaggcggc gcatcgatgc
    20581 ttcaatgccc cctatcagca ccggggagga aggaaacagc ttcttaagta tctgagtata
    20641 aacaacagtc gcgtagtcag gcctgaatcc tgccattcct ccgggagtat atgcatcatc
    20701 tgatctcagc ctgcgggctg cagtgtaatg gttgaccatc gagtccatgt tgcccgcagt
    20761 gactgcaaag aaataccggg gctcacccag cttggtgaaa tcacgaaggt catccctcca
    20821 gttgggctgg ggtaccacgg caaccttcat gccggctgac tcaagaacgc gggcaattac
    20881 ggctgccccg aaagaagggt ggtcaatata agcatcgccg gtaaacagta tgacatcagg
    20941 cctctcccac cccgctgcct ccatctcctt cttcgaagtg ggaaaccaga tcatcgcact
    21001 attttccctg tttttcaccc gcccaagtta catataatgg ataaattgct cccaattgta
    21061 acagaaaagt cttaccttca tctgtcagcg tcaattatca ggtcaatgta cggacaattc
    21121 aatagattga gaggtacggc attatgtttc ttgctgttga ttattgcgtt atgcggaaat
    21181 ccggctccgc ttcctgccca gcaaaataac gccaggccca gcgtgggact ggctctcagc
    21241 ggcggaggcg cgaagggcct tgctcacctg ggagtgatga aggtgatgga agaagcaggc
    21301 ctgaaacctg actatgtcac cggagtaagc atgggaagca ttgtcggggg aatgtatgcc
    21361 atgggataca cacctgacag catagcccgg atgttcagaa tgttcgactg ggaatatgcc
    21421 atgtcagaca tgatccctga agacaagatc atctttcttg aaaagagaca ctttcataac
    21481 agccttattg ctttgccgat cacccgtgaa gcaatcaaga tcccgtctgg actgatcagc
    21541 ggccagcaga tagagagcgg actgaaccat tatttctggc ctgcagccac aatcaccgat
    21601 ttttccaaac tccctatccc cttcctctgc ctcgcaactg atgtggtaac cagcaaaaag
    21661 gtggttttcc gcagtggcta cctcccggat gccatacggg caagcatggc catcccctcg
    21721 gtattcacac ccgtcagaac agatacggca gtccttgttg acggaggcgt ggtacgcaat
    21781 tatgctgcaa ccgaactgcg tgagatggga gcagatatag taataggttc atatgtgagc
    21841 ttcaggggat acaaggagaa agacctggga accgcctacg gaatactgaa gcagatcgga
    21901 ttcctcagca gcctggctga ctatgaagag cagaagcggc agaccgatat catgattgag
    21961 ccggagctgg gagaagtggg taccctttca ttcaataacg tagataccat aatttccagg
    22021 ggctaccgtg aggccctcaa atacagggat gtctttaaaa ggctggccga ctcccttgac
    22081 gccatctgtc cgcgtgaacc tgtaaaggcg ctgcccgacg tccggtatta tatttttgac
    22141 tctatcatgg tcacaggtaa cgaaataatc agtgatgacc agataattgg cgttctcgac
    22201 gtaaggccgg gagaaaacgt tgacagggat ctcctggagg agagaattga attgctgtac
    22261 ggaaaaaact ggtttgaaaa ggtccggtac aggatcgttc ccggagaaga agagctgaca
    22321 ctggaaatcg aatgcattga acgtcccagg gcaatgctgt acgggtcact gcattatgac
    22381 ccggctctca gtgcaggtgc ggtggtaagc ctctcggtga gagacctgat cactccaagg
    22441 tctgtcatta accttgattc gtatattggt caattctacc gggtcaggct gagcgcgact
    22501 cagttcgtag acaaaagcca gaaattcggg gttgaattct cattcttcgc tgacaatacc
    22561 cggttgccgc tgattaaact gaaaaatgag acaggaccga tgctcaacca taatttcaat
    22621 acttcattga gcctgagcaa acgcctgagt ctgaatcacc tgatgaccct gacagcctcg
    22681 tttgagaacc agcaccttgt ccctgacttc cttacatcaa ccctgatcac caggttgtct
    22741 tatgactacc tcaggctgaa ctacagctac caggccaaca cccttgacag gaaacacttc
    22801 cctcagcagg gcatcacttc cgggatctcc ctgtcagcac tcagaatgat cagaggtgcc
    22861 gtcagaaccg gatctagaag ttcagtgtac ttcccggacg acgaagattc accattcagc
    22921 tttgaccgca actattcggc aagggcatgg attaattcct attcacctgt ttcagagaaa
    22981 gtgacattaa tgctgggtgg cgatgtgttg tttaccacag gtacagattc cgtaacttcc
    23041 ggaaatgact tctggctcct tggcggaact gaccccctgt ctgaccgctc catcactgct
    23101 gtcggattcc accctaacca gattactgtc agaaatgtgg gaggattgcg cctcggcact
    23161 gacatagaaa taaccaagga ctttcacctc acctttgatc tcaacctgtt tgccctgaaa
    23221 gagccctacc gcgaggccgg cttcaccatt atggggggtt acgcaatcgg cgcaggctat
    23281 atgacagttg ccggacctgt ccggatcgga atcatgcacg gcctgtatga ccggcaggta
    23341 ctgtacaggg ctttcaaggg atacgtaagc cttgggttca acttctgaac cccggatgtg
    23401 gcctgattgt tgtcttatta ccaggggcgg tcagcacaac acagattatg aatatcaata
    23461 cagtcatact gaagcacagg agcgtctgca acctcatcgt tcagagaaag gtgcgccaga
    23521 gctttgagat catgtcagac atgcttgata atgtgtctgt tagcgatctc agggatcatt
    23581 tctctgagct gcagatgact tacaggaata ttctccggta cactgttgag ggcatacatg
    23641 accccgaaag gcagaaggtt tacaaccggc tcctgcaaaa tatactcagg ctcaacgaca
    23701 gaatcaaaca ggacattttg gcacgttatt cagggtggta cacttattct gtcagggccg
    23761 gtgagcagaa ggaagaaaga ctcaggggag ggaatatcat ccagagtgtg gatgaccttg
    23821 tattcaaatc acgccttgat gatctccttg cctctgctga gataggccgg accggccccc
    23881 gcagtgaggc agcaatggag gttgaacgcc tctcagggat aatattcaac catctctggc
    23941 tgaccgatta ttacggtgaa gctgaggaga gtcttatgga tctccttata ggatcggccc
    24001 ggtttgagtg gcatgagata gcaacattcg tcagtgctgt gacattgagc agtctccggg
    24061 tctgggatcc ggccaaaatc aggctgctgg ccaggctgac tgctgacctt acaccacagg
    24121 tggctgaaag agcagtcaca gggctggtct tcagccttca ttattatgat gaccgcctga
    24181 acctctatcc tgaggttatt cagacagtca gagacctggc aaaggtgaag aacttcaggg
    24241 aaagatgccg catagtggtg ctccaggcta tcagatcacg ctatacagaa cagcttagcc
    24301 ggaggatgaa cgaggagata ttgccgaaag tcgtcaaact taaaccccgg atcgaagaaa
    24361 aactggacct tgacaaaatt ctgggcgaaa atcccgaaga gggccagaat cctgactggt
    24421 cagaaatgtt ccgggggtca gaggaaatgt ttaaaaccat ggaggaactg actaacctcc
    24481 agatggaagg atctgatgtt tatatgtcag cgtttgcagg attgaagaag ttcgacttct
    24541 tcagggaact gcgcaactgg ttcatcccct tctaccctga tcatgaggca gtagactcaa
    24601 tcttcaggga tgaaatcctc ggtcccggca taaacgagct ggccgaggcg ctgtacaaaa
    24661 ccccattcat ctgcaactcc gacaaatttt ctctcatcct taatatgaaa tacctgccat
    24721 ccgaacagaa gagcatgatg ctcaaggtgt tcaggatgga acttgaggga ctggaacaga
    24781 tgaaatatga caatgaactc actgatcctg ctgcagtttt caggactacg gtgacccagt
    24841 atgttcagga tctctaccgg ttcttcaagc tctctgattt caggcatgag tttgacgatt
    24901 ttttcacagg aaggcttgat ctgtacaact ccctttttta cagggaaacc tgtggctcag
    24961 ttactgacga caggtcactg gctgactatt tcttcagcaa ggagtactat gaggatgcac
    25021 tggctctcta tctgaccatt ctgaagggcg tcaccgatga tgcggaactg tacgaaaagg
    25081 caggttattg ctaccagaag gctggagaat acgggaaagc actggagaag tactcaatgg
    25141 ccactattat cgaacctcat acatggacgc ttcgcaagac aggctggtgc cttcgaaggc
    25201 ttggcaggcc ggctgaggcc ctggaggcgt atcgcagggc actgcagaat gaacctgacg
    25261 acctgaacac tgtccttatg gttgcccact gttgccttga caccgccaat tacgatgagg
    25321 cgctgaaaca ctacttcagg gtggaatatg aaagccctgg aaattcaaag gtgctgagac
    25381 caatagcgtg gtgttacctg gtcacaggca ggtataccga agcggagaag tacttcgaga
    25441 ggcttgccgg gaccggcctg acgccctatg acaaaataaa catggggcac cttgccctat
    25501 gccagggaag cacgaggata gctgccgaac attatttaag tgccatgacc ggaggagaaa
    25561 tgtctgcaga gagcttcctg tcagcgttta atgatgatat tcctgtactg gcggcaaatg
    25621 gcgtcaatcc tgacgacctg cccatcctgc tcgattacgt tctgatgaac ctgaaaaagg
    25681 agaactgacc tcttctccct ggtcattccc gcctttaaga ggctggtgcc tgggtctcag
    25741 tacttctatg gtcatgtcct tgcggtagtg aagaatgaac atccccctgt tctcgtagcc
    25801 gttgttacgg ctgctgcggc tgaagtcagg ctccaggcac agaagttcag gattaaaggt
    25861 gccgggatcc ttcaggaaac tcttcccgcc ggaggctata cctccgataa agtaccatgc
    25921 gatgtcaaaa ccgcgccatg cgaagctttc agccatgggc tcggtcctga atttcttcat
    25981 atacatggct gaaaatgatt ttaccccggg tgcctcatag tcgacatagc tttctgacgg
    26041 tatatgcagc tcaagatcat aataatactt caggtcaact gtttcaaggc cgcgtatttc
    26101 aggatacccg atcaccatta tatcatacct ctttgtcagg ctgtggagtg cggagaacgc
    26161 tgaactgacc acaggtgtac tggttgtggc cagcactatt atgttcttcc gtgcatgatc
    26221 aagctggctt tcaaatgagg caacggtgct gtaaacattt cgcttttccg tcaccccggt
    26281 atagaagtat ggcacaactc tcccaccgcc tgagatggca gaatcaccgg tcgcctgttt
    26341 caccttctgc cacaggctgg cagttgccgg gtcatacata gccgtatcgg aatagaggaa
    26401 gatgaccctg cttccctggc gtgacgcaac ttcatcggca attaaatcct gcaccacttc
    26461 ctggcttggg aaaaccctgt acagcgaagg cttgttctca tgtatgtgac tgtcgcgaag
    26521 cggcaccggt gatacaacag gaatatcccg ttcggcggcc cagccggcaa tctgttgcag
    26581 attataggag aacacaggtc cgattatcag gtcaacatca tccagttctc ctgtccgtaa
    26641 aagtcttttg atttccgcag agtcagcccc ggtatcataa accttcatct caacggtcag
    26701 accaagtgac cgcaggctgt cagcggctat cagcacgcct tcgtaagcct ccaggaaggg
    26761 gaggctaccg tcataaatct gagagccagt cctcaccctc tctgtggtat ctgcataggc
    26821 tctggcagtg ttttccctca cgaagaaggg aaacagcact gccaccctta cggttccgtc
    26881 cagtctgtca atcctgtttt tttcaccagg catggtaaaa gtctcctgtt catcaatgac
    26941 ggccatggtg tctgctattg ctgtatcagc aggtattccg gcgggcacct gctcccgtcc
    27001 cgctctttga tcctggctcc tgtaggaagc cttgtttttg ggtataagga ggtaatcacc
    27061 ctcgtgcggg aagagcagtc ccctgtttgc ccttttcagg tctctcaccg tgatgccgta
    27121 atcattggca attgttccca gtgtctctcc tcttcttacc atgtgataga taaaccgatc
    27181 atcctgggaa gactgcttct gtggcacctg ggcgcctgca ccggcgacag ccggcttacc
    27241 ctggccgggc tgaccggact gggctgcctg ttgtgcctgc ccggtttgcc ctgccgtacc
    27301 ggtctgcgta ccggccgggt ggttcttcat tgcagcctgg ccctggtcca ctgccgcagc
    27361 aggtatcctc agcacctggc catcctttat ggcattgtca accaatccgt tttcgcgggc
    27421 caccatgtca acactcacgc tgtaagccct ggctattgaa tagagcgtct gccccttcat
    27481 tacctggtgc atgtaataaa ccctgccctc cactactatc ttctcaccgg agagagtaac
    27541 gggaaccgga accggggtct gtgtctggga tttaagcgac gcaggcatca gcagcactgc
    27601 aagaagcagc agcgaagccg tccgccacca ggagaaaaat ctcatttttt cttccatcaa
    27661 ttgattcatt cgtattactg tcaaccccct gtattatctc acttacagct gtttaagata
    27721 gctgttgacg ttccgctcta tccttcccag gggatcttcc gtatcagcct ttacaaattc
    27781 cctgccggta atgtgttcat agagctcaat gtaacggtct gatatctccc tgactatttc
    27841 aggtgtcatc tgcggcacct tttcaccgct tcttccctgg aagccgtttg ccataagcca
    27901 ctcacgcaca aactcttttg atagctgcct ctgctgttcg ccacgggcga atctgtcctc
    27961 gtaaccatct gaataaaagt accttgaact gtcaggggta tggatctcat caattagata
    28021 aattttcccg tcccgtttcc cgaactcata cttggtatcg acaagtatca gtccctgctt
    28081 cgcggctatt tccgagcctc tcatgaacaa cttcatggaa tagtcctcaa gcttccggta
    28141 ttcctcctct gaaaccagac cgctgctgat gatcgcctct gcagagatat cctggtcatg
    28201 catcccctgt tctgccttgg tggtcggtgt aatgagaggc ttcgcgaatt tctggtgctc
    28261 tctcatccct tccggaagag gtattccgca tatctcccgt ccgcctgctt tgtatagtct
    28321 ccaggaactt cctgtcaggt aacccctgac tatcatctca acgggatatg tctggcacct
    28381 gaccccgacg gtaacattgg gatccggaga tgcgatgttc cataccggaa cgatatcact
    28441 caccatttca aggaacatgg tcgctatctg attgagaacc tgtcccttgt atggaatccc
    28501 ctcaggcagc acaacgtcaa atgccgagat acggtcagtg gctaccatca ggaggtattt
    28561 gtcatcaatg ttatagacgt cgcgaacttt tccccggtat actcccttct gccgcgggaa
    28621 attgaaatct gttccggtaa tagactttgc catggatctg cgaattattg ctgtgattct
    28681 tcctttctgt ttttctcatc gctgtcataa gcccttacta tatgcttgac aagccgatgc
    28741 cttactatat ccctctcatc aaaacggata atggaaatcc cttcaattcc ttccaggagg
    28801 tgcattgccc ggagcaatcc cgactcgctc cgtttcggga ggtctacctg cgtggtatcg
    28861 cctgtcagga tgaacttgga gctcacgccc attcttgtga ggaacatctt gagctggctc
    28921 atggttgcat tctgggcttc gtcaagtatg acgaagcagc tctcgagagt ccttcccctc
    28981 atgaacgcca gaggggcaat ctgcaccgtt ccatcctcta tccactgctc cagcttgcgg
    29041 aacgggatca tgtcatgcag ggcatcatag agaggctgga gatagggatc aagtttgtcc
    29101 ttcatgtccc cgggcaggaa gcccagccgt tctcctgcct cgacggcagg gcgggtgagc
    29161 acgatcttct taacctcgtg gttcttcaga gccctgacag ccagagcgat ggcagtatat
    29221 gttttcccgg tgccggccgg accttcggca atgataagat cattgccgga atactctttc
    29281 acaagctgaa gctggttgac agtgcgtgcc ctgataggcc gtccgctggt gccgaataca
    29341 agagcatcct cgaaatcgcc gctggaggct gaccgcatgt gttcgtcatc aaggatcaga
    29401 cgctcgagat caccctcatc aagccggttg taacgcctgt aataatcaat gagttcattc
    29461 atgcgtgaga cgaaaacgga aacctcctca gcctcaccca cacatttgat ctcatttcct
    29521 ctggctatca gcctgacttt cggaaaataa ccctgaatct gatcaagcag cctgttgcca
    29581 ggccccagca taaccatcgg atcaataccg tcaagcaaaa ttacctgttc cgtcatctgc
    29641 ctctcttctc cagctctttc taaagaattc tccttaagct tcttatgtcc gtgatcgttt
    29701 ttgtgcatcc gttctaccgc aaagtaagtt tattttttgc agaagattat ctttgctgta
    29761 acaattttag gatgccgctt atcacgctaa ccaccgagtg gagggaggat gatttcttca
    29821 acgggatact cagggggaaa cttgcctccg cctgtccggg tgctgtaatt gtgaacaacg
    29881 ccggcggaat ccctccactg aacataatgc acggcgcatt cgtcataaga aacaccttca
    29941 gccattatcc cgaaggctcc atccacctgg tattcgtcag cagcgaggga cctgcagaac
    30001 acccccacct tctggtcaaa gcacacaacc actggtttgt gggtgcaaac aacgggatgt
    30061 tcaacctgat tctcaattcc gaacctgacc tgatcatcag cctcaggaat gatgccgggg
    30121 cagacgagat cacactcttt gccgtcaccg ccgcggcaat agccaaaggg caaccaccgg
    30181 agagcctcgg ggaaagtatt tcatccgtct ccgaaaaggt gccgctgaga gcaacaatag
    30241 accgcaatgt gattatcggc agcgttatct tcattgactc atacggcaac gcaatcacca
    30301 acatcacccg ggagatcttc gccagagtct tcgagggaag ggactacagg attctgatca
    30361 agagcaacaa ctactttacc gagaaaataa gcaggttcta ccgcgaagaa cctgtcgggg
    30421 agatcgtgac ccgcttcaac tcactcgacc tgctcgaact ttctatcaac ggggcaaacc
    30481 tgtgccagct tttttccgtc gataccggtg acgccgtaag ggttgaagca agaaaccagg
    30541 gccgtgaaac aaacggcctc ttttaaaacc tttaataaat gagagaaaaa actatcaggg
    30601 aattcaccag gctgctcgat atcatggatg agctgcggac gaagtgcccg tgggacatga
    30661 aacagaccaa ccagacactc aggaaactga cgatagagga aacatatgag ctggcagatg
    30721 ccatactgca ggacgacaat gatgcaatca aaaaagagct tggcgacatg atgctccatg
    30781 tgacattcta tgcaaagatc ggatcggaga aaggactctt tgacatgggc gatgtgcttg
    30841 aaagcatcaa caacaaactg gtataccgcc atcctcatgt cttcggagat gtgaatgtca
    30901 cacggggagc tcgcgaagtc gaggagaact gggaaaaact gaagatgacg gagcacgctg
    30961 aatacaaacc cgtactttct ggtgtgcccg catcgctgcc ggcaaccgta aaagccaacc
    31021 ggatccagga aaaggtgaga ggtgtgggat tcgactggga ctcgcgcgaa cagatatggg
    31081 acaaggtgac ggaggagctt gatgaactca aacaggaaat tgcttccgga agaaaagtgg
    31141 atatagagtc tgagcttggc gacctgttct tctcccttat aaatgctgca agactatacg
    31201 agatagaccc tgaatcagcc ctcgagaaaa ccaacaggag gtttatgaaa agattcaact
    31261 atctggaaca gaagacactg gcaaaaggtc tctcgctgcg cgaccttacc cttgatgaga
    31321 tgaatgaaat ctgggaggag gccaaacagt ttgataaacc tctttagctg ctgaaaagct
    31381 gcgcgctgaa cctgtagctc agcatctggt agatcacctt cgcagccatg aaatcagggg
    31441 ccttgtttga ttccgaagga cagagttcca caacatcgaa tccgaccaca ttacgtttgc
    31501 tgaacacatc acgcaggaag aacatcagct cacggtagtc aggaccgcca ggctccggag
    31561 tgcctgtcga cggcatcacc gaaggatcga acacatcgag gtcgacagtt atgtatacat
    31621 tccccggcag cagatccagc gccttgtggt aaagagtctt atcgctgtaa agttcatggg
    31681 cgaagaagat gctcttcctg tctgcaaaag ggagttcctc tgctgacatg cttctgatgc
    31741 caacctgcac cagcggtgca tgctccttcg cccttgcagc agcacaggca tgattcaggt
    31801 gtgatccttc ataagaaggt ctgaggtcag aatgagcatc aagctgcagg attgccaggt
    31861 ctgtgaaggc ttcagacgcg gcacggaaag ctccgatact gacggtatgg ttgccgccga
    31921 taaccaccgg gaaacgattc tctctgaaca ggcgcgagac ggtgcggtaa acctctccgg
    31981 tgacctcttc cggatcatca cctgtaagaa gcggggccag ggtatggatt ccgtacctgt
    32041 gtacctcggt tccggtttca atatcataaa actccagcgc cggcgaagct tcaagaatgg
    32101 cagcaggccc cctgtcggca cccctgatat atgtgctggt ggcgtcatat ggcacaggaa
    32161 ctattatgat cttcgactca tcataactgt atatgacctc ttcgcctccg aagtttgtca
    32221 ctgccattgt gctactcccc tgaggccttt ggtttatctc cctttgcagc aggggccttg
    32281 cggctttttg gagaggtttt ctcacctgct ccggctttag ctgccttgcc tgtttcggct
    32341 tttgctgtct tgcctgcttc ggcttttgct gtcttgcctg cttcggca
//
LOCUS       DAOPXU010000024        31076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_1404_length_31076_cov_12.215274, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000024 DAOPXU010000000
VERSION     DAOPXU010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 31076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31076
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1404_length_31076_cov_12.215274"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(333..2477)
                     /locus_tag="PKK03_03920"
     CDS             complement(333..2477)
                     /locus_tag="PKK03_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNX83574.1"
                     /translation="MRKLLAIIVILVTFSASQARADEGMWLLPLIEKLNIGTMTEMGL
                     KLTAEDLYSINQASVKDAIVIFGGGCTAEIVSPHGLLLTNHHCGYGQIQNHSTVEHDY
                     LNNGFWAMSKEEELPNPGLSVTFLVRIEDVTSRVLGKVNPDMTEAARREAIQAESAAI
                     TSEATKGTHYNARVSSFYSGNQYFLLIYEVFSDVRLVGTPPNSIGKYGHDSDNWMWPR
                     HTCDFSVFRVYMGPDGKPAQYSKDNVPLKPKHYLPVSIKNLQKNDFAMVMGYPGTTTR
                     YMTSYEVDEAMKITNANRVKIRGARQEIWMKDMMADPKVNIQYASKYAGSSNYWKFSI
                     GQNAGLTRLRTAEKKAAFEAEYMKWVNADPARKAKYGNALSLIENAVKGRAEKYNVSQ
                     YMREALSTELLGFAGQMTMLEEALVKKDAERINGMTGRLKRSVENFYGDYNYPTDQKT
                     TKAMLKLYRTDIDPKYWPSFYKLIDTKFKGNIDAFVDNIFAKSIFVSQEKLAAFLEAP
                     SLKVLQKDPAFIVAKSINEMNSTLMKDLQGFSADLSKGQRLYMAGVMEYQPDKTQYPD
                     ANSTMRLTYGKVLDYYPYDAVHYNWYTTLDGVVQKYKKGDYEFDLPQRLIDLNNKREY
                     GRYGSPDGYMPVCFITNNDITGGNSGSPVINGNGELIGLAFDGNWEAMTGNIAFEPDL
                     QRCINVDIRYVLWVIDIYSGAGYLLNEMDIRQ"
     gene            complement(2554..3156)
                     /locus_tag="PKK03_03925"
     CDS             complement(2554..3156)
                     /locus_tag="PKK03_03925"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNX83575.1"
                     /translation="MLKPFFRDGIREAGCDEAGRGCLAGPVYAAAVILPKRYRNKELN
                     DSKKLTARQRERLRTEIISSATTWCVASCSNTEIDEMNILRASITAMHRAIDGLEPRP
                     LLLLIDGNRFYTYGDIPHQTIIKGDATYLSIAAASVLAKTERDHRMEELDALCPCYGW
                     RQNKGYPTPEHRRAIAEFGPSPFHRLSFNLIDLQRELPFL"
     gene            complement(3156..4289)
                     /locus_tag="PKK03_03930"
     CDS             complement(3156..4289)
                     /locus_tag="PKK03_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNX83576.1"
                     /translation="MIIAINARTLRAVPRDGIGWFTLEVVSGMVRNHPEHRFVLIGDR
                     RPEPLPVRGDNVEYVTLPLRTVHPLLWHAWHEYLLPPVLRRTGADVFIAPDGMIPLRT
                     DVPCIPVIHDLNHEHRPGDIPGAECRYYRRLFPLFAGKGARVATVSHFSAGDIAATYG
                     ISPDKIDVVPNGVAGIFSPPQPGEAEETRRHYTGGKPYFLFVSNFSPRKNVEAVIRAF
                     EIFRQSGGGEQVLLLAGRRLYLTREMDRHLEASPYGKDIVFTGSVTRDVLRRLYGAAE
                     ALTFVPWFEGFGIPVVEAQRCGVPCILSDSSSLPEVSGGAALCVSPADAGAIAGAMAR
                     LVTDGALRRRLADEGYANSLRYTWEQAAEEMWKSVIKATGGAA"
     gene            4371..5822
                     /locus_tag="PKK03_03935"
     CDS             4371..5822
                     /locus_tag="PKK03_03935"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNX83577.1"
                     /translation="MRRKFLINITFLLTVSILVKAFWVLGIDRTVQNVVGETAYGSYF
                     SLFSFSVLFTLLLDLGLSGFNNRAVSADPARVRIYFGNVLVIRLFLTAVYFLVSISVA
                     YALGYREGQITILLVLMLNQVMASMILWLRSSISGMQYLFLDSLLSVADRLVMIVICS
                     VLLWGGVTTGGFRIEWFVWAQTAAYFTVMCAAFIIVVRKGRVAAVKPDTSVLKSIIMT
                     GLPYSVVVFAMTLYWRMDSVMIERLLPDGATRAGNYAQAFRLFDALAMIPVLFGGMLL
                     PIMTRGLSSDSDIRPLARMAGRMLIVPLGIGAVTLATFPAEMLDILYTSPVENAVTAF
                     RILMITLVPVGVIYIYSTMLTASWKLKMLGVITVSGMIINLSLNRILIPELGPSGAAC
                     TALVTQSLVAVACMLTVRRTMSGIAGNGRILLFLLMLGLTFLAGWLLSRTGIPWTAAA
                     ALQLAAGILLALLFRFIEPLSSLKLITGRFGTA"
     gene            5864..6391
                     /locus_tag="PKK03_03940"
     CDS             5864..6391
                     /locus_tag="PKK03_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83578.1"
                     /translation="MKRFITGIFLLMICTYLFPQKSDRLSHQVLVPAGNIVTHTGLTY
                     QQTIGETAVEVSLPSFYNLSQGFQQPRFIPEQKLPDREGNGVDFFPNPLTESSEAGPW
                     VLTIRVYGVMARHYRIFIYTLPGSLMYTDELDLSAEHDYRHKVSLVHYNKGIYVANVI
                     STDGVINRSFKIEKL"
     gene            6402..7346
                     /locus_tag="PKK03_03945"
     CDS             6402..7346
                     /locus_tag="PKK03_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83579.1"
                     /translation="MKRVSSYAMAILVVLVAATLTAGAQGTVPAGINYQAVARDNSGN
                     ELVSTPIEVRFSIRKESPTGEIIYQEVFTNVTTSRYGVFSLMIGQGAPVLGTFGAIDW
                     STANHYLTVEVKFENLFMDMGTMQFMAVPYALYAAKSLERGPAGPAGPKGDPGDPATD
                     TDDQKLIYDQGTKLLSIESGNTVRLDTIIAFRARNLLSDIAPQITNVIMTYDEPDLSI
                     GGGFNAGTGIFTAPADGIYTFNVSYFADGNGGSREVSIYVNSAPYEKLAVEIGSGTTM
                     TVRSITIRLSRGNTVSVNIYTGTATQTGTGSFAGYKVG"
     gene            complement(7361..8530)
                     /locus_tag="PKK03_03950"
     CDS             complement(7361..8530)
                     /locus_tag="PKK03_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX83580.1"
                     /translation="MEKLFEHFIKLLKETDTRFLRYIYPEINWENRMIGLTGPRGVGK
                     TTLVLQHIKMNLDIPETLYVTAEDFYFSDNRLTDLADAFVKRNGKYLFVDEIHKYKDW
                     ARELKLIYDYHPELKIVFSGSSVLDIKKGASDLSRRAVMYNLQGLSFREYLQLFHHVA
                     SRTFTLEEILSHKAGLPQIKHPLPLFEDYLKRGYYPFSLEEDFERKLQQIVNHTLETD
                     IPVYAGMNVSTGRKLKQLLAIIAKSVPFKPNMTSIATALVASRNNIADYCLFIEEAGL
                     ITQLRDATGGIRGLGKVDKVYLDNTNLIYNLASVDPNPGNIRETFFLNQLRLKYDVIT
                     SPAADFRIDDFTFEVGGKSKGLKQIQGLDNGFIVRDGIEHGYLNIVPLWHFGMIY"
     gene            8761..9853
                     /gene="prfB"
                     /locus_tag="PKK03_03955"
     CDS             join(8761..8823,8825..9853)
                     /gene="prfB"
                     /locus_tag="PKK03_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_131840564.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNX83581.1"
                     /translation="MITNDQIQDLVERRDALRRYLDVDARLNEIKEKEEITHSDGFWN
                     DPKKAEELMKEISSVKSWTTAFAKVDTAVEDMLVLYDFFKEGEVTDEEMGQQYDKTLA
                     AVEKLELRNMLRREEDRLGAVLKINAGAGGTESNDWAQMLMRMYLMWGEKSGYKITEA
                     YYQAGDEAGIKTVTLEFEGENAYGYLKSENGVHRLVRISPFNAQGKRQTTFASVFVAP
                     LVDDDIVVEINPADITWETFRSSGAGGQNVNKVETAVRLRHAPTGLVIENQESRSQGQ
                     NKENALRLLRSHLYEMELRKRMDEQAKIEGAKKKIEWGSQIRSYTLHPYKLVKDLRTG
                     YEAGNAQAVLDGEIDEFIKAYLMEFGGKE"
     gene            9928..11793
                     /locus_tag="PKK03_03960"
     CDS             9928..11793
                     /locus_tag="PKK03_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNX83582.1"
                     /translation="MDKKKKLFEQFPPVSATEWMEKITADLKGADFTRKLVWKTRDGL
                     SVMPFYRQEDLDTLPHSNLLPGDFPFVRGVAVKGNNWFIRQDITVTDYKAANAKALDI
                     LMRGITSLGFVIADSQTATPETVAQLLKDIHPESIEISFSIAGRAKELVAALKGYLRS
                     SGADLKQVNITVSADPLGRLAANGKLCVPVDQGLDYLAGLVSDAAETPGIKCVEPSGT
                     LFSNAGAGPVAELAYTLSLGNEYMAALTGRGIDPDTAAGAFRFTLAIGPDFFPETAKL
                     RAARMLWAEIVSAYGTKEPGSALMHIHSVTGRWNKTLYDPYVNMLRTQTEAMSAVLGG
                     AESVTVEPFDTVFRTASDFSERIARNQQLLLMEEAHLGKIADPGAGSYYIEELTSMTA
                     REAWKLFLEIENEGGFLAALRKGMIQQRISEAAATRKADMAKRKEVLLGTNQYPDFRE
                     KKSPEHDPGKLFKEYAASADEEVRPILPARGADEFEKLRLATEKSGRRPLVFMLTIGD
                     LAMRRARAQFSSNFFAVAGYEVQDNNGFAGVAEGVNAAIGAKADIIVICSSDEEYATL
                     AKEAFKATGGKAVFVVAGNPPCTEDLKTEGVEHFISIKSNVLETLQMFNRILGIN"
     gene            11800..13956
                     /gene="scpA"
                     /locus_tag="PKK03_03965"
     CDS             11800..13956
                     /gene="scpA"
                     /locus_tag="PKK03_03965"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HNX83583.1"
                     /translation="MSKRPKFTDIDPLKVTVPSRDKQKWEKENGISPQWETPERIRLK
                     SVYSREDLKGMEHLQYAAGVPPYLRGPYSAMYTMKPWTIRQYAGFSTAEESNAFYRRN
                     LAAGQKGLSVAFDLATHRGYDSDHERVVGDVGKAGVAVDSVQDMKILFDQIPLREMSV
                     SMTMNGAVLPVMAFYIVAGLEQGAKLSELQGTIQNDILKEFMVRNTYIYPPAFSMRII
                     ADIFRYTSEKMPKFNSISISGYHMQEAGATADIELAYTLADGLEYLRTGVNSGMNIDD
                     FAPRLSFFWASGMNFFMEIAKMRAARMLWAKLVSQFNPKNPKSLALRTHTQTSGWSLT
                     EQDPFNNVGRTCIEAMAAALGHTQSLHTNALDEAIALPTDFSARIARNTQIFIQEETS
                     ICKSVDPWAGSFYVETLTHEIAHKAWELIEEVESLGGMAKAIESGIPKMRIEEAAARA
                     QARIDSGAQTIVGLNRYRLEKEDPLEILEVDNSAVREAQLRRLAEVKAGRNGEECRRA
                     LEAITRAAETGEGNLLELAVDAASKRATLGEISYACEKVAGRYKAVIRTISGVYSSEY
                     KSDSDFEEARAMAAEFAARAGRQPRIMIAKMGQDGHDRGAKVISTGYADIGFDVDIGP
                     LFQTPSEAARQAVENDVHLLGVSSLAAGHKTLIPQVISELKKLGREDILVIAGGVIPP
                     QDYQFLYDAGVAAIFGPGTSVAKTAKQILSLLLHTV"
     gene            13956..14606
                     /locus_tag="PKK03_03970"
     CDS             13956..14606
                     /locus_tag="PKK03_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNX83584.1"
                     /translation="MRTTFAILLVAAIGVLAGCSASRKNRAVAGESSAEAVAEQQSPE
                     AKVPDVAPFGNVALAGKPEGDEATAHVWDFRDATTWLLGDLSLAMLNNPPHYEWYQKG
                     YAAYQPDQGVMAGLMELGKDNLTITIVLGTWCPDSRREVPHFIKMTDLWGFPHERIKF
                     IGVDINKIAPLADYPQLAIERVPTFIFYVNNIEKGRIIEVPVTSLEQDTKNILEGN"
     gene            14628..15119
                     /locus_tag="PKK03_03975"
     CDS             14628..15119
                     /locus_tag="PKK03_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNX83585.1"
                     /translation="MEEEKSPRQSGVGVGALVTAIIAFLLAVIPCIGLVAVIPAVIAI
                     VLAIIGLSRPQVNQGMLVGGLVVGIIALMISVSQSFVLGKIANKSGSWATDIEKVIRD
                     VTDDLEKEFGDNEVTIKINSDDETVEIKASGKKGGLEDRLEELEGTVDTLKSKADTTK
                     GQW"
     gene            15116..15532
                     /locus_tag="PKK03_03980"
     CDS             15116..15532
                     /locus_tag="PKK03_03980"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HNX83586.1"
                     /translation="MKPNPDTKPYLILNLILAGVILLIMGYSLFYSPDENEYPVPCIH
                     EKLTGEPCPSCGLSHAFSLIVRGRVDEALAWNSQSLRVFIFFALQLVMRLGLALTAIK
                     AVGNLRRVAVTDAAVSGVMALTAFYPFLRILWISLF"
     gene            complement(15569..16042)
                     /locus_tag="PKK03_03985"
     CDS             complement(15569..16042)
                     /locus_tag="PKK03_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNX83587.1"
                     /translation="MAEKKQREELLQRAMRACSEREYCISDIAALLDRWGADGAETRT
                     WIIERLIKEKFIDEQRYSRAFVVDHFRHSQWGRVKISMALRQKRVDPEAIASGMEAIG
                     EDEYLELLQKILGDQRRKIKAKNKLDLKGKLLRHALGKGFESHLVYDAINSQLSE"
     gene            complement(16035..16901)
                     /gene="prmC"
                     /locus_tag="PKK03_03990"
     CDS             complement(16035..16901)
                     /gene="prmC"
                     /locus_tag="PKK03_03990"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNX83588.1"
                     /translation="MAGKLQTISEISRRITELLKAGWPAEEAAALASAILQEYTGMGR
                     AAQMAFGERVAEAGAAARIENAVARAAAGEPLQYILGHTEFCGHRIEVAPGVLIPRPE
                     TEEMTMMIIGENSGFAGTVTDLCTGSGCIAIALSLAFPDADITATDLYHAALNVAIRN
                     ITRTGAAVTLVQADILNDDPGHVPASNIIVSNPPYVTGQEKAAMHGNVLNHEPHEALF
                     VPDDNPLLYYRAIAAVAETRLLPGGTLWLEVNENLAGETAALLSPETYREVRVLKDIR
                     EKERFIKAEKHG"
     gene            16927..17976
                     /gene="ribD"
                     /locus_tag="PKK03_03995"
     CDS             16927..17976
                     /gene="ribD"
                     /locus_tag="PKK03_03995"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNX83589.1"
                     /translation="MTRPDDLIWMRRALQLASMAEGCTSPNPMVGAVVVHDSVIIGEG
                     YHLKAGTPHAEVHAINAVSDRSLLPSSTIYVSLEPCSHQGRTPPCADLIISSGIRRVV
                     IGTPDTNPLVAGRGIARLRQAGIEVITGVAEEECRRLNRRFFTWHEKKRPYVILKWAR
                     SADGFIDLRRKASDAIGPNWITGMAERVLVHRWRATEDAILAGGGTIRADNPSLTVRY
                     WSGRSPVRIIVSRSGNLDPDSSVFDGSAETVLFTCNGQAQFPDTRTVTLTGAEPCVSE
                     VLANLWQAGIQSLFVEGGAFIISSFLESGQWDEARRFTGTANFVDGVPDPFPGFTPAK
                     TVFFDKSILDFAYHF"
     gene            18073..20454
                     /locus_tag="PKK03_04000"
     CDS             18073..20454
                     /locus_tag="PKK03_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX83590.1"
                     /translation="MKRGFIVLVLLLVVTCMNLAGQAARKYIRAGEEFTRNGMLEDAI
                     EQYSKAIDEDPSSADGYVRRARVHEMTGSYGLACDDYRRANNFLPDDAGILHNLGRMC
                     NMLGASAKATPEEKNKFYSEAVSYLQAAIRKEFRNGKLYTEKVVSLIGMEQWDRALGA
                     SDTAIQMRDDAVNYYHQGIIYQRKGDDNTARRQLEKAVTKDKSFAAARLELARVLVRQ
                     GDVNSALGQCNMVIQQDQKNVDAYLTRAMVYEAKLDYPSAINDVSTAILLEPAGQQHY
                     VTRGICYQKFNQHLAAVSDFTRALTIDQFTPEIYLMRARSYEEIHDFEKAADDYTRVV
                     AMNENSSSAKKLLTVANDRLYAINRENNSPEIWLETPTVTPKSEIEIGLDKKSIMVTG
                     KVTDKSLLAGFSINNIPVRYEGKGTIPFAAEVEIPSDETLRIVARDVYDNEKVLTYRI
                     RRTEVDPPEIQILAPYILGENVLVIDSIASTIDIEGRVKDQNLIRSVKIENISAQFSQ
                     DNLNPAFIAKGIDVRNNDKVTILVEDVYGNRTIMDYALDRNGTTISASNPMGNTLVVF
                     IENSDYQNFAALQGPPKDASLITGVLEDDYQVLPVKRIRNQTKQEMEKFFSIDLRDEV
                     RAKQIKSLVIWFAGHGKFINDVGYWIPVDARRDEEFTYFSLNTLRASMETYMNYLTHV
                     LVITDACESGPSFYQAMRSDARPRNCADWRATQFKSSQIFSSAGYELAVDDSQFTRTF
                     ASTLRGNPNTCISIDEIVLHVTKAVTGNNQQKPRFGKITGLKDEDGTFFFVKK"
     gene            20459..23704
                     /locus_tag="PKK03_04005"
     CDS             20459..23704
                     /locus_tag="PKK03_04005"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNX83591.1"
                     /translation="MRGRGSNSGKYSITVLALLLLSQLILPPRLYSQTFYFEQYGSKE
                     GLSASKVYTIIQDANEFIWLGTGSGVTRFDGLRFENFSTSDSLSPGGVKSICEDSRGR
                     LWLGHIGGGVSIIEDNIIRRASFSPSIFIGVDSARISDPLRPINSLTGDVTGITEYDG
                     SIWVSTSLGGALRLDMPERGVSLMTGQQYVGRQGLSNDAFGLYADHRENLYCITDMGI
                     KKYDHASDRFIPFNPEGLTRYFLTTTIFEDSRGNMWFGTFNGGLYRQNGETGEMGFFD
                     TRNGLWGNFVSYILEDYRGNIWVASMEDWESRGGITVFSPEGTVVYNSTNGLPALHIL
                     CMIEDKEKNILIADRDAGLFIYKGDHFASFMAPQFMPSNDVFTLAQDHTGRYWFGTGR
                     GLSLYNPALKEEDRIKLSGFPPSLEGTQISIIRPDNEGHVWIATAGDEVYRYDIYEKN
                     FRYDLDLNASLRSLRIAAMTTDLRDNLWIGTDRALIMWNPQLKQSTTYTQINGLTGNN
                     ITALFCDSRGLLWIGTEERNGLTRFNPGNNTFTPLTFRSDVHPTAITETPDGMIWVGT
                     IDGLYSLSNDTVKKKLTEAEGLLMNSINLLQPDRSGNLFIGTNLGLNRYDSNTGRISS
                     FTEKSGFTGIETRPNASFADDMGDLWFGTSNGAMRLSPSMLPPVVTEPATHIWRTLVN
                     YEVFPMHDGIRLLHTQNRIIFDFLSICLVNPGAVRYQFMLEGADKDWITTDQNRAAYS
                     SLSPGKYTFLVRASNNYGYWNEVPAKLSFSIRGPVYMTSWFIILCITALVAGIYLFIS
                     LRERTLRREKEHLEQKVEERTAEVVSKSLELEEKNRDTTASIRYAERIQAAMLPPENF
                     FNDTFVLFMPRDIVSGDFYWMHDSGFKQYIAAVDCTGHGVPGAFMSIIGHNSLNKIVR
                     ESGITKPSDILDHLNIEVVHALLQRSEKAVRDGMDISVISYDILEHTVEYAGAYQPLY
                     HVRDGKVTIYRGDRFPIGMIDGQARKSFTNNRVDVKPGDMLYIFTDGFADQFGGEHEK
                     KFKTINIRSLLAEIHMLPVEEQKRRLEKAIREWMGGLAQVDDILFIGTRVP"
     gene            complement(23743..24318)
                     /locus_tag="PKK03_04010"
     CDS             complement(23743..24318)
                     /locus_tag="PKK03_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNX83592.1"
                     /translation="MKVCVFAASSSRIDAAYMKAAEELGVAFAGHRVHAVFGGGGIGL
                     MGIFADSLMAHGGSVTGVIPGFMMDEGWGHSGVSDMIVTPDMSERKKTIFAMSDAAVA
                     LPGGVGTLEELTEVITLKQLGQFNKPIVIINVNGFYDHLIEFFDHMVRGHFMRLEHRD
                     IWQIVTTAEEVVPAIVNYTGWHADPKVIARI"
     gene            complement(24326..24643)
                     /gene="trxA"
                     /locus_tag="PKK03_04015"
     CDS             complement(24326..24643)
                     /gene="trxA"
                     /locus_tag="PKK03_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNX83593.1"
                     /translation="MAQQVNDSNFDEVVLKSDKPVIVDFWAEWCGPCRMIAPYIEQIA
                     EAYREKALVVKCDVDSSPDVARRFMIRNIPTVLYFKNGEVVDKQVGAAARSAFEAKLT
                     PLL"
     gene            complement(24689..28174)
                     /gene="dnaE"
                     /locus_tag="PKK03_04020"
     CDS             complement(24689..28174)
                     /gene="dnaE"
                     /locus_tag="PKK03_04020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNX83594.1"
                     /translation="MSLFVHLHVHTQYSILDGASAVKTLVRRAKELGMTALAITDHGN
                     MFGVKEFHDAATAASIKPIIGCEIYVARTSLEDKTSVEDRSGDHLILLAKNKTGYRNL
                     VRLVSEAWIRGFYYKPRIDKELLRKHREGLIASSACIAGEAAQEILTGNIRAAEESIL
                     SYREIFGDDYFLEIQRHETTDPTSDVTVFPQQQRVIAAYRELAAKHNIKIIASNDVHF
                     IMKDDAEAHDRLICLNTGKDIDDQKRLRYTKQEYLKSAEEMLEIFADIPEAVSNTVEL
                     ADRVENYKLNSEPIMPEFTIPGEFADKDEYLRHLTMKGAAERWNTITPDIQARIDFEL
                     SVIKNMGFPGYFLIVQDFLRAAREMGVWVGPGRGSAAGSVVAYSLRITDIDPVKYNLL
                     FERFLNPDRISMPDIDIDFDEDGREKVLRYVVEKYGHDRVAHIITFGTMAAKMAIRDV
                     ARVQKLPLPEAIRLTKLVPDRPGTTLASAYEEVPELLKEKESKEPLVAQTLKYAEVLE
                     GSIRQTGVHACGIIIGKDSLSEHIPICTAKDTDLYVTQYEGNHVESVGLLKMDFLGLK
                     TLSIMKDAVDNIEKSKGITIDLQTLELDDRITYELFSNGETTGVFQFESVGMKRYLRE
                     LKPNQFGDLIAMNALYRPGPMDYIPQFIRRKHGKEKISYEVPAMEKYLKETYGITVYQ
                     EQVMLLSQELGGFSKGQADSLRKAMGKKKRDIMDRLKEQFIEGCRMNGYDQNTINKIW
                     SDWESFAEYAFNKSHSTCYALIAYQTGYLKAHYPAEFMAAVLSRNISDIKKITVMMEE
                     TRRMGTEVLGPDVNESYLQFTVNRNGNIRFGLGAIKGVGENAVLHLIEEREKNGPYKD
                     IYDLAERVNLNTVNKKMLEAMVVSGALDGLPGMTRAQYFAADGKGSSFLESLIRFGNN
                     VKNIRNSTQQSLFGDTGGFDLVRPEPAPCPDWSKLERLNKEKEVIGIYLSSHPLDDFR
                     LEISTFCNATLADLQNLSEFANREVCVAGIVSDTRSGVTRNGKPFGGFTLQDYTDSFS
                     FLLFDKDYVAFSNYFRNDYQLLVKGRVQGRHFNPEELEFKIKEIHLLTAVREDLITSI
                     TIKLKPELVNPEFIKNLKSVISENPGNKSLKFLLIDHDERITIPLFSRSIKAGITDEL
                     IGWLEDNPELGFKVN"
     gene            28342..>31076
                     /locus_tag="PKK03_04025"
     CDS             28342..>31076
                     /locus_tag="PKK03_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX83595.1"
                     /translation="MKRLLSVFFLLCGLLLTSAAQQKTVTGTVTASEDNLGIPGVTVQ
                     VKGTTLGVVTDIDGRYSIQASQGQVLVYRFTGQKTQEVTVGAGNTIDVVMLPDLLNLE
                     EVIVVAYGVQKREASTGSVGEVKSSEIQGIPETSIEKMLTGKVAGVQVTASSGQPGAS
                     TQIRIRGISSINAGTEPLYVIDGIPVMSGDQSYYTNTGNALASLNPNDVESMTILKDA
                     AASSIYGSRAANGVILITTKSGKAGASKINFRASYGIEQLANDNGYRPLTAEEYLTYQ
                     RTAVINAGGNPDDPTNAKYYFPMSLLNGPITDWKKELTRTGSIYSAELSAQGGSDKTQ
                     HFFSALYDKYEGIVYGSDFTKFSIRSNVDHQLNKKLKLGVRFNGAYSHANDVAMQSLY
                     YVNPMFAMIMIKPFTPLRNEDGSYNLNIPENANSNPRATAQDDSQWEKQYRLNTNGYL
                     EYQIAKGLTAKTTNSIEMTDGEGRRYWSPEANYGETMGYLQSSKTKYVLLTTSNTLNF
                     NKEFGSHTVSVVGGQEASKFDYNEYSIISPNVDPNIPFPNTSTSDDDDADYYESAYTI
                     LSFFGVANYNFDNKYYLQASLRTDGSSRFGSKNRWGTFWSVGGSWNVHNEDFMDNVDW
                     INMLKVRGSYGISGNFNIGNYDQYGLYGAGQYNGSAVMVPTQPSNPDIGWENNSEYNA
                     GFDFALFDRITGSFDVYSRKTLDMLLGYPLSRTSGFSSITTNIGSIKNEGIEFMLDGT
                     LIATDKFEWTAGFNIAHNNSTILDLGKDEQFVNSNNTRILHKVGEHLYSFYLFDYAGV
                     NPANGEAMWYNADGELTNKYSEARRVIAGSPEPKLNGGINTKLTAYGFSLDLNLEYKY
                     GNLICLTENRYSNSDGYSWPNNYGNTQLDYWTTPGQIARNPKPVANNASSSSASLS"
BASE COUNT         7580 a         8105 c         8047 g         7344 t
ORIGIN      
        1 ccggtcttag atttgatatc caggatgata gggatcgatc ccggcatgtc aatgattgcg
       61 aacaacacca tgaagcaggc taatatgtca aggagattaa actgcattgg ggaaattttt
      121 gcaaaggtac tctttttaag gcattaggca agataggggc gagcgtgaat aaaatgcccg
      181 gggggcattt ttagcgagca gccagattgc aggggagggc agtagtgctg gcaaaggatc
      241 aaggcctgtg gttacagttc attaagaatg aaaaagggat ccaattctgg aatccctttt
      301 ttcactaatg cctattgctt tttgcctgca ggttactgcc tgatatccat ctcattgagc
      361 agataccctg cccctgaata gatgtcaatc acccagagaa cgtagcgtat gtctacgttg
      421 atacatctct ggaggtcagg ttcgaatgct atgtttccgg tcatggcttc ccagttgccg
      481 tcaaaggcaa gaccgatcag ttcgccgttg ccattaatga ccggactgcc tgagttgcca
      541 ccggtgatgt cattgttggt gatgaaacat acaggcatgt agccatcagg tgaaccgtaa
      601 cggccatact cccttttgtt gttgagatca atgagccgct gcggcagatc aaactcataa
      661 tcacccttct tgtatttctg cacaacgccg tcgagggtcg tataccagtt gtaatgaact
      721 gcatcatacg ggtagtagtc gagtacctta ccgtaggtga gcctcatggt ggagttggca
      781 tcagggtact gtgtcttgtc aggctggtat tccattactc cggccatgta gagacgctgc
      841 ccctttgaga ggtcagcact gaatccctgg agatccttca ttaatgtcga gttcatctcg
      901 ttgattgatt tggcaacaat aaatgcagga tccttctgaa ggaccttcag gctgggagcc
      961 tcgagaaatg ccgccagttt ctcctgtgag acaaagattg acttcgcaaa gatattatca
     1021 acgaaggcgt cgatgtttcc cttgaatttc gtgtcaatca gcttgtagaa cgatggccag
     1081 tacttcggat caatgtcagt cctgtagagc ttcagcatcg cctttgtggt tttctggtcg
     1141 gtaggatagt tataatcacc gtagaaattc tcaacagatc tcttaagcct tcctgtcatg
     1201 ccgttgattc tctcagcatc cttttttaca agggcctcct cgagcatggt catctggccg
     1261 gcgaagccca acagttctgt cgacaatgct tctctcatat actgggaaac gttgtacttt
     1321 tcggcccttc ccttaacggc gttttcgatc agggaaagtg cattgccgta ttttgccttg
     1381 cgggccggat cagcgtttac ccacttcatg tattcagctt cgaaagccgc tttcttttct
     1441 gcggtgcgca gacgggtgag accggcgttc tggccgattg agaacttcca gtaattggat
     1501 gaaccggcgt atttggatgc atactggata tttaccttcg ggtctgccat catatccttc
     1561 atccatatct cctggcgtgc accacggatc tttaccctgt tggcgttggt gatcttcatt
     1621 gcctcgtcaa cctcgtacga tgtcatgtac ctggtggtgg tgccggggta gcccattacc
     1681 attgcaaaat cgttcttctg caggttcttg attgagacag gcaggtaatg cttcggtttg
     1741 agcggaacat tgtccttcga gtactgagcc ggtttcccgt caggacccat atataccctg
     1801 aaaacgctga agtcgcaggt atggcgcggc cacatccagt tatcagagtc atggccgtat
     1861 ttcccgattg agttaggggg tgttcccaca agcctgacat cggagaaaac ctcatagata
     1921 agcaggaaat actggtttcc gctgtagaat gatgatacgc gggcgttgta gtgggttccc
     1981 ttcgtagcct ctgaggtgat ggcggcgctc tcagcctgga tggcttcgcg gcgtgccgct
     2041 tcggtcatgt ctggattgac cttgccaagt acccttgagg taacatcttc aatgcgcacc
     2101 aggaaggtga ctgacaggcc gggattggga agttcctctt cctttgacat ggcccagaaa
     2161 ccgttattga gatagtcatg ctcaactgtg ctgtggttct ggatctggcc ataaccgcag
     2221 tggtggttgg taagaagcag gccgtgagga gagacgatct cagcggtaca gccgcctccg
     2281 aagatgacga tggcgtcttt aacgcttgcc tggttgatgc tgtacaggtc ttccgctgtc
     2341 agcttgagcc ccatctcggt catagtgccg atattgagct tctcgatgag cggcagcagc
     2401 cacatgccct catcagccct ggcctgcgag gctgagaagg ttacaaggat aacaataatt
     2461 gccagtagtt ttctcatgat ttatggtgtt gttgatttaa gtttggaacc aaagatatca
     2521 aaatacattc tcaggaataa tgattttaac attttataag aaagggagtt cccgctgcag
     2581 gtcaatcagg ttgaatgata gccggtggaa cgggctgggg ccgaattcag caatggctct
     2641 gcgatgctcc ggggtaggat atcccttgtt ctgccgccac ccgtagcagg ggcagagggc
     2701 gtcaagctcc tccatgcgat ggtcacgctc cgtctttgcc agcactgatg cagccgcgat
     2761 ggacaggtat gtggcgtcac cctttattat tgtctgatgg gggatatctc cataggtgta
     2821 gaacctgttg ccgtctatca ggagcaggag cgggcggggt tcaagcccgt ctattgccct
     2881 gtgcatggcg gtgatagagg cgcgcagtat gttcatctca tctatctcgg tgtttgagca
     2941 cgaggcgacg caccaggtgg tggcagatga tatgatttcc gtgcggagcc gctcgcgctg
     3001 gcgggcggtc agtttcttgg agtcattgag ctctttgtta cggtatctct tcgggaggat
     3061 gactgctgca gcgtacacgg ggcctgccag gcatccgcgc cctgcctcgt cgcaaccggc
     3121 ttcacggatg ccgtccctga agaatggttt aagcatcagg cggctccccc ggtagctttg
     3181 atgactgatt tccacatttc ttccgcagcc tgttcccagg tgtacctgag tgagttggca
     3241 tatccctcgt cagcgagacg gcggcgcagc gcgccgtcgg tgacgaggcg tgccatagcc
     3301 ccggctatgg cacctgcgtc agcggggctg acgcagagtg ccgcgccgcc gctcacctcg
     3361 gggagtgatg agctgtctga cagaatgcac ggcacaccgc atcgctgcgc ctccactacg
     3421 ggtatgccaa acccctcgaa ccacggcacg aaggtcagcg cctccgcagc accgtagagc
     3481 cggcgcaaca catcacgcgt tacagaaccg gtaaagacaa tatccttgcc atacggggag
     3541 gcctccaggt gcctgtccat ctcacgcgtc aggtaaagcc tccgcccggc aagcaacaga
     3601 acttgttctc ctcctccgct ctgcctgaaa atttcaaatg ccctgatcac cgcttcaaca
     3661 ttcttcctcg gagaaaaatt gcttacgaac agaaaatatg gcttaccacc ggtgtaatgc
     3721 ctccgcgtct cctccgcctc gccaggctgc ggcggagaaa aaatcccggc aacaccgttc
     3781 ggcacgacat ctatcttgtc cgggctgatc ccgtatgtcg cagcaatgtc gcctgccgag
     3841 aaatgtgata cagttgcaac acgtgctccc ttccccgcga agagcgggaa aagcctgcgg
     3901 taatacctgc actcagcgcc aggtatgtct ccgggccggt gctcatggtt gaggtcatgg
     3961 atcactggaa tacatggcac atccgttctc agcggaatca tcccgtcagg tgcgatgaaa
     4021 acatcagccc cggtccgcct cagcaccggt ggcaacagat actcatgcca tgcatgccag
     4081 agcagcggat gcactgtccg taacggtaag gtcacatatt caacattgtc acccctgacc
     4141 ggaagcggct ccggcctcct gtcgccgatc aggacaaacc ggtgctcagg gtggttacgg
     4201 accatcccgc tgacaacctc aagggtgaac cacccgatac cgtcacgggg cactgccctg
     4261 agcgtacgcg cattgattgc aatgatcatt ttccgctgct gctactgaaa tacaaatgtg
     4321 gcaattaatt tataaattgg cattgattct tttatccacg gcagactgat ttgaggcgaa
     4381 agttccttat caatatcacc ttcctcctga cagtgagcat cctggtcaag gctttctggg
     4441 tgctgggcat agaccgcact gttcagaacg tggttggcga aaccgcctac ggaagctatt
     4501 tttcactttt cagcttctca gtgctcttca ccctcctgct tgacctggga ctctcgggat
     4561 tcaacaaccg cgctgtatcg gctgatcctg caagggtaag gatctacttt ggcaatgtgc
     4621 tggtaataag actctttctc acagcggtat acttccttgt ctctatcagc gttgcttatg
     4681 ccctgggtta ccgggaaggg cagataacaa tactgctggt actcatgctg aaccaggtga
     4741 tggcatcaat gatcctctgg ctccggtcaa gcataagcgg catgcagtac ctcttccttg
     4801 acagcctcct ttccgtagcc gacaggctgg tgatgatagt catctgctcg gtgctcctgt
     4861 ggggcggagt tactaccggt gggttcagga tagaatggtt tgtatgggcc cagacagctg
     4921 cctattttac ggtgatgtgc gctgcattca taatagtggt gcgcaagggg agggttgcag
     4981 cagtgaagcc tgatacctca gttctgaaga gcatcatcat gaccgggctg ccctattcgg
     5041 tggtggtctt tgcaatgaca ctctactgga gaatggattc ggtgatgatt gaacggttgc
     5101 ttcctgacgg ggccacccgg gccggcaact acgcccaggc cttcaggctc ttcgatgcgc
     5161 tggcaatgat tcctgtgctg tttggcggga tgctgcttcc cataatgaca cggggactgt
     5221 catcagacag tgatatcagg cctctggcaa ggatggccgg ccggatgctc atagtcccgc
     5281 tggggatagg tgcggtgacc ctcgccacgt ttccggctga gatgctcgac attctctata
     5341 cctcaccggt tgagaacgcc gtgaccgctt tcaggatact gatgattacc ctcgtacccg
     5401 ttggagtgat atacatttac agcacaatgc ttactgcttc atggaaactt aaaatgcttg
     5461 gggtaataac agtgtcaggc atgatcatca acctttcact caaccggatc ctcattccgg
     5521 agctgggccc gtcaggagct gcctgtaccg ctctggtcac tcaatccctg gttgccgtgg
     5581 cttgcatgct gaccgtgaga aggacgatgt ccggcattgc cgggaacgga aggattcttc
     5641 tgttcctgct gatgcttgga ctgaccttcc tggccgggtg gctcctgagc aggacaggta
     5701 tcccgtggac agctgcagcc gccttacagc ttgcggcagg gatcctcctg gccctccttt
     5761 tcaggttcat cgagcctctc tccagcctca aactgatcac cgggaggttc ggaactgcct
     5821 gaaaagtgtt atttttgaat ttgtaaacaa aagggccttt atcatgaaac ggtttattac
     5881 cggtattttc cttttgatga tatgtacata cctcttcccg cagaagtcgg acaggctgtc
     5941 gcaccaggta cttgtgccgg ctggtaacat tgttacacac acaggtctga cttaccagca
     6001 gacaattggc gagacggccg tggaggtgtc acttccctct ttttacaacc tgtcacaggg
     6061 ctttcagcag ccaaggttta tcccggagca gaaactgcct gacagggagg gaaacggggt
     6121 tgacttcttc cccaatccgc ttaccgaaag cagcgaagcc ggcccctggg ttcttacaat
     6181 aagagtctac ggagtgatgg caagacatta caggatcttc atctacaccc tgccgggctc
     6241 actgatgtac actgatgaac tcgacctttc tgctgagcat gattacaggc acaaagtcag
     6301 ccttgtccac tacaacaaag gaatctatgt cgccaacgtg atcagtaccg acggcgttat
     6361 taacaggtca tttaagattg aaaaactata aaccaaaggg tatgaaaaga gtctcttctt
     6421 atgcgatggc aatactggtg gttctggtgg cagcgactct tacggcaggg gctcagggaa
     6481 cagtcccggc cggtatcaac taccaggccg tggcccgtga caacagcggc aacgagctgg
     6541 ttagcacgcc aatagaggtg cggttctcta tcaggaaaga gtcacccacc ggtgagatca
     6601 tttaccagga ggtttttaca aatgttacta cctccaggta cggggttttt tcgctcatga
     6661 ttggacaggg cgctccggtt ttaggcacct ttggtgcgat agactggtca actgccaacc
     6721 attacctgac tgtggaggtc aagtttgaga atctgttcat ggatatgggc acgatgcagt
     6781 tcatggctgt gccgtacgcg ctctatgccg ccaaatcact tgaacgcggc ccggccggcc
     6841 ctgccggccc caagggtgac ccgggcgacc cggcaacgga tactgacgat cagaaattaa
     6901 tttatgacca ggggacgaag cttctctcaa tcgaatcagg aaacacagtc cggctggata
     6961 caattatagc attcagggca agaaaccttt tatcagatat tgcccctcag attaccaatg
     7021 tgataatgac atatgatgaa cctgacctga gcatcggagg tggtttcaat gcagggaccg
     7081 gaattttcac agccccggct gatggcatct acactttcaa tgtatcctat tttgcagacg
     7141 gcaacggagg ctcacgtgaa gtttcgatct atgtcaactc cgcaccttat gaaaaactag
     7201 ccgttgaaat tggaagcggt accactatga cagtcaggtc aataacaatc agattaagca
     7261 gaggtaacac tgtaagtgta aatatttata caggtactgc aacccagaca ggcacaggct
     7321 ccttcgccgg ctacaaggtt ggttaaaggc ttacggtccc ttagtagatc ataccaaaat
     7381 gccatagagg aacgatattc aggtaaccat gttcaattcc gtctctgaca ataaagccat
     7441 tatctaaacc ctgaatctgc ttcaaccctt tgctttttcc accaacctca aaggtaaaat
     7501 catcaatcct gaaatcagct gcgggagatg taattacatc atattttaac cttagctggt
     7561 tcaggaaaaa tgtttccctt atgttcccgg gattcgggtc gacacttgcg agattgtaaa
     7621 tcagattagt gttgtcgaga tatactttgt ctactttgcc caaacctctt attcccccgg
     7681 tggcatccct gagctgggta atcaatccgg cttcttcaat aaacaggcaa taatctgcaa
     7741 tattattccg gctcgcgaca agagcagttg ctatgcttgt catattaggc ttgaatggaa
     7801 cgcttttagc aataatagcc agtaattgct ttaactttct cccggttgat acgttcattc
     7861 ctgcatacac cggaatatca gtttcaaggg tgtggttgac aatctgctga agttttcttt
     7921 caaaatcttc ctccagtgaa aatgggtagt accctctctt cagatagtct tcaaagagtg
     7981 gtaatgggtg ttttatttga ggtagccccg ctttatgcga caggatttcc tccagggtaa
     8041 acgtccggga agcaacatgg tgaaacaatt gcaaatattc cctgaacgat aaaccttgca
     8101 ggttatacat aaccgccctg cggctcaggt cagaggcccc ttttttaatg tccagaacag
     8161 aagaacctga aaacacaatt ttaagttccg gatggtaatc gtagatcagt ttcaactccc
     8221 tggcccagtc cttgtattta tgtatttcat caacaaacaa atactttccg ttacgtttaa
     8281 caaaagcatc agccagatca gttaacctgt tgtcagaaaa gtaaaagtcc tctgctgtca
     8341 catagagcgt ctcaggaata tccaggttca ttttgatatg ctgcaatacc aaagtcgtct
     8401 ttcctacccc acgagggccg gtaagcccta tcattctgtt ttcccagttt atttcaggat
     8461 agatgtaccg caggaaacgg gtatctgtct ctttcagaag ttttataaaa tgctcaaata
     8521 acttttccat ggcagttaat attctgcaaa gatataataa tgcaccataa aaggtcacat
     8581 atacattaaa aacgcactat tatagtgcaa atattgccca tttggatata attatacacc
     8641 tgaatgcggg gcgtccggtc ccgaaatacc atgtcggccc ccagacgccc gcaccactac
     8701 tggttattac cagctggctc ttgcctaatt tgactatatt tgcgcaaact tttacatgac
     8761 atgataacca atgaccagat acaggacctc gttgaacgcc gggatgcgtt aaggaggtat
     8821 ctttgacgtc gatgccagac taaacgaaat caaggaaaag gaagagatta cccactcgga
     8881 cggattctgg aatgacccga aaaaagcaga ggaactcatg aaggagatat cctctgttaa
     8941 gagctggacc acggctttcg ccaaagtcga cactgccgtt gaggacatgc tcgtcctgta
     9001 cgacttcttc aaagagggtg aagtcaccga tgaggagatg ggtcagcaat atgataaaac
     9061 cctcgcagca gtagaaaaac ttgagctccg caacatgctg cgccgtgagg aggaccgcct
     9121 cggcgccgtc ctcaagatca atgcaggcgc cggtggcaca gaaagcaatg actgggcaca
     9181 gatgctcatg agaatgtacc tcatgtgggg agagaagagc gggtataaga tcacagaggc
     9241 atactaccag gcaggcgacg aagccggaat aaagactgta accctcgagt tcgagggtga
     9301 aaacgcctat ggatacctca agagcgagaa cggcgtccac cgcctggtcc gcatatcacc
     9361 cttcaacgca cagggcaaac gacagacgac attcgcatcg gtcttcgtcg ctccgctggt
     9421 tgatgatgac attgtcgtcg agatcaatcc ggccgatatt acctgggaga ccttccgctc
     9481 atcaggcgcc ggcggccaga acgtcaacaa ggtggagacc gccgtccgcc tccgccatgc
     9541 ccccaccggc ctcgtcatcg agaaccagga gtcacgttcc cagggacaaa acaaggagaa
     9601 cgccctccgc ctcctcaggt cacaccttta tgagatggaa ctgcgcaaaa ggatggatga
     9661 acaggcaaag atcgagggtg caaagaaaaa gattgaatgg ggctcacaga tcagatcgta
     9721 taccctgcac ccatataagc tggtaaagga cctgcgcacc ggttacgagg caggcaatgc
     9781 ccaggccgta ctcgacggtg agattgatga gttcatcaag gcgtacctga tggagttcgg
     9841 cggcaaagag tgaactctca tgataaaaag catggtttaa cagccttaca acacattaat
     9901 ttacaaccct cagcaacata aatcctgatg gataagaaaa agaagctatt tgaacaattt
     9961 ccgcctgtct ccgcaacgga gtggatggag aagatcactg ctgatctgaa gggcgctgac
    10021 tttaccagga aacttgtctg gaaaacacgc gacggccttt ctgtcatgcc cttctacagg
    10081 caggaggacc ttgacactct gcctcacagc aacctgcttc cgggtgattt cccctttgta
    10141 aggggagtgg cagtgaaggg taacaactgg ttcatccgcc aggatataac ggtgacagac
    10201 tataaggctg ccaacgccaa agctcttgac atactgatga gaggcatcac ctcgcttggc
    10261 tttgtcatcg cagactcgca gacggccacc ccggagaccg tcgcacagct ccttaaggat
    10321 attcatccgg agagcattga gatcagtttt tcaatcgccg gcagggcgaa agagctcgtt
    10381 gccgcactta agggatacct ccgctcatca ggcgctgacc tgaagcaggt gaacataacc
    10441 gtctcggctg acccgctggg caggctggcc gccaacggca agctgtgcgt gcccgttgac
    10501 cagggcctcg attacctggc aggcctcgtc agcgatgcgg cagagacacc gggcattaag
    10561 tgcgtcgagc cctcaggcac ccttttcagc aatgccggcg ctggccccgt cgctgagctg
    10621 gcatatacgc tgtcactagg caatgaatac atggcagcac tgaccggaag aggcattgac
    10681 cctgacacag cggcaggagc cttccggttc acactcgcca tcggccccga cttctttcct
    10741 gagacggcca aactgcgtgc agccaggatg ctctgggcag agatagtcag tgcctacggc
    10801 acgaaggaac ccggatcagc cctgatgcac atccattcgg tcacaggcag gtggaacaag
    10861 accctctatg acccgtacgt caacatgctc aggacccaga ccgaagctat gtcggctgtt
    10921 cttggcggcg cagagtcagt aacagtggaa ccattcgata cagtgttccg gactgcaagc
    10981 gacttctcgg agaggatcgc ccgcaaccag cagctgttgc tgatggaaga ggcgcacctc
    11041 ggaaagattg ctgacccggg agccggatca tattacatag aggaactgac ctcaatgaca
    11101 gcccgcgagg cctggaaact attccttgag attgaaaacg agggaggatt ccttgcagcc
    11161 cttcgcaaag ggatgataca gcagcgcatc agtgaggctg ctgcaacgcg caaggctgac
    11221 atggcaaaaa gaaaggaagt ccttctgggt acaaaccagt atcctgattt cagggagaaa
    11281 aaatcgccgg aacatgatcc gggaaaactt tttaaagaat acgctgcctc agctgacgag
    11341 gaggtaaggc ctatcctccc tgcccgcggt gcagatgagt tcgagaagct gagactggca
    11401 acggaaaagt ccggccgcag gccgctggtt ttcatgctca ctatcggtga tcttgccatg
    11461 cgcagggcaa gggcacagtt ctcatccaat tttttcgcag tggccggcta tgaggtgcag
    11521 gacaataacg gttttgcagg cgtcgccgaa ggcgtcaatg cagccatcgg agcaaaggca
    11581 gacataattg tcatctgcag ttctgacgaa gaatatgcca cacttgcgaa agaggccttt
    11641 aaggcaacag gcggaaaggc tgtctttgtg gtggcaggaa atccgccatg cacggaagac
    11701 ctcaagaccg aaggagtgga gcacttcatc agcatcaaat ccaatgtgct tgagacactg
    11761 caaatgttca acaggatact cggaataaac tgacgggaga tgagcaaaag accaaaattt
    11821 actgatattg accccctgaa ggtgacagtg ccttcaagag acaaacagaa gtgggagaaa
    11881 gagaacggca tcagcccgca gtgggagact cccgaaagaa ttcgcctcaa gagcgtttat
    11941 tcccgtgaag acctgaaggg catggagcac ctgcagtatg ctgccggtgt accaccgtac
    12001 ctccgcgggc cttattcggc catgtacacc atgaagccat ggacaatcag gcagtatgcc
    12061 ggattctcca ccgctgaaga gtcgaacgcc ttctaccgca ggaaccttgc tgcaggccag
    12121 aagggactat cggttgcttt tgaccttgca acacaccgcg gatatgactc tgatcatgag
    12181 cgcgttgtgg gtgatgtagg gaaagccggc gtggcagttg actcggtgca ggatatgaag
    12241 atcctctttg accagatccc cctcagggag atgtcggtat cgatgacaat gaacggggcg
    12301 gtgctgcccg taatggcatt ttacatagtg gcagggctgg agcagggagc aaagctgagt
    12361 gagttgcagg gcactattca gaatgatatc ctgaaggagt tcatggttcg caacacatac
    12421 atctacccgc ctgcattctc gatgcggatc atcgccgaca tattcaggta cacctctgag
    12481 aagatgccga agttcaacag catctcaata tcggggtatc acatgcagga agcaggcgcc
    12541 acagcggata tagaactggc ttataccctg gccgacggcc ttgagtacct ccgcaccggg
    12601 gtgaattccg gcatgaacat agacgacttc gcgccgcggc tctctttttt ctgggccagc
    12661 ggcatgaact tcttcatgga gatagccaag atgagggcgg caaggatgct atgggcgaag
    12721 ttagtcagcc agttcaaccc gaagaatccg aagtcactgg cgctccggac ccatacccag
    12781 acatcgggat ggagccttac cgagcaggac ccgttcaaca acgtcgggcg tacctgcatc
    12841 gaggctatgg ctgcagcgct gggccatacc cagagtctgc acaccaacgc ccttgacgag
    12901 gccattgcac tgcctaccga tttctctgca aggattgctc gcaacaccca gatattcata
    12961 caggaagaga ccagcatctg caagtcggtt gacccgtggg ccggttcatt ttatgttgag
    13021 acactcactc acgagatcgc acataaggca tgggagctga tagaggaggt tgagagtctg
    13081 ggtggcatgg caaaagccat tgagtcaggc atacccaaga tgaggattga ggaggcagcc
    13141 gccagggcac aggcaagaat tgactccggc gctcagacaa ttgtcggcct caacaggtac
    13201 aggctggaga aggaagaccc gctcgagatc ctcgaggttg ataacagcgc tgtgcgcgaa
    13261 gcccagctga ggaggcttgc cgaagtgaag gcaggacgta acggggaaga gtgccgcagg
    13321 gcgctcgaag ccatcacccg tgcagccgaa accggtgagg gaaacctgct cgaactggcc
    13381 gttgatgctg catcaaagag ggcaacactc ggcgaaatat cttacgcatg tgaaaaagta
    13441 gcagggaggt acaaagcagt gataagaacc atttcaggag tatattcatc agagtacaaa
    13501 tcagattctg attttgagga agcaagggca atggcagccg agtttgccgc ccgggcggga
    13561 agacaacccc gcatcatgat tgccaagatg gggcaggacg gccatgaccg cggcgccaag
    13621 gttatatcga ccggatatgc cgatattgga ttcgacgtcg acatcggacc acttttccag
    13681 actccgtccg aagcagcaag acaggcagtg gagaacgatg ttcacctgct cggggtctcc
    13741 agcctcgccg caggccataa gacccttata ccgcaggtca taagcgagct taagaagctg
    13801 ggccgggagg atattcttgt gattgcaggc ggggtcattc cgccgcagga ctaccagttc
    13861 ctctatgacg ccggtgtggc agcaatcttt ggtcccggca cctcagttgc gaaaacggca
    13921 aaacagatat tatctttgtt gttgcatacg gtatgatgag aacaactttc gccatactat
    13981 tagtggcggc catcggtgtc cttgccggct gcagtgccag ccggaagaac agggccgtgg
    14041 ccggggaatc ctcagctgag gcagttgcag aacagcagtc gcctgaggca aaggtccctg
    14101 atgttgcccc cttcggcaat gttgctcttg ccgggaaacc tgaaggagac gaggcaactg
    14161 cacatgtatg ggatttccgc gatgccacca catggctgct gggagatctc tccctggcga
    14221 tgctgaacaa ccctccgcat tacgaatggt accagaaagg ctatgctgcc tatcagcctg
    14281 accagggcgt gatggccggg ctaatggaac tcgggaaaga taacctgacc atcacaatcg
    14341 tgctcggcac atggtgcccc gatagcagga gagaggttcc gcactttatc aaaatgaccg
    14401 acctgtgggg gttcccgcat gaaagaatca aattcatcgg ggttgacatc aacaaaattg
    14461 caccccttgc agattaccct cagctggcaa ttgagagggt cccgacattt attttttatg
    14521 taaataatat cgaaaaggga cgcatcattg aagttcctgt gacgtcttta gaacaggata
    14581 caaaaaatat ccttgaaggt aattaggaac aattaatact tgaaccaatg gaagaagaaa
    14641 aatcccccag gcagtcagga gtgggtgtcg gtgcactcgt aaccgccata atagcattcc
    14701 tgctggcagt tatcccctgc ataggcctgg ttgcagtgat cccggcagtg atcgccattg
    14761 tccttgccat aatcgggctt tccaggcccc aggttaacca ggggatgctc gtcggcggac
    14821 ttgtcgtagg cattatcgcc ctcatgatat cagtttcaca gagctttgtc cttggcaaga
    14881 ttgcaaacaa atcgggcagc tgggcaacgg atattgagaa ggtaataagg gatgttaccg
    14941 atgacctcga gaaggaattc ggtgacaacg aggtaaccat caagatcaac agcgatgatg
    15001 agaccgtgga gataaaagct tccggaaaga aaggtggtct cgaagaccgg ctggaagagc
    15061 ttgaaggaac cgttgacacc ctgaagagca aggctgacac cacaaaaggg cagtggtgaa
    15121 accaaacccg gatacaaagc cgtatctaat tcttaatctt atacttgccg gagtaatcct
    15181 gttgatcatg ggttactccc ttttttattc ccctgatgag aatgagtacc ctgtaccgtg
    15241 tattcatgag aaactgaccg gtgagccgtg tccctcatgc ggattgtcac atgcattttc
    15301 acttattgtc cggggcaggg tggatgaggc cctggcttgg aacagccagt cgctcagggt
    15361 attcatcttc tttgcacttc agcttgtgat gcgcctcggc cttgcactga cggccataaa
    15421 ggcggtgggg aacctgagaa gagttgccgt cactgatgct gccgtttcgg gcgtgatggc
    15481 cctgactgct ttctatccgt tcctcaggat attgtggatc agcctgttct gagacctgag
    15541 gagcctgaag ccgtttaatg aaagaatctc actcacttag ctgtgagttg atggcgtcat
    15601 agacaagatg gctctcaaag cctttcccca gggcatgcct gagaagcttt cccttgaggt
    15661 cgagcttgtt ttttgccttt atctttctcc gctggtcgcc caggatcttt tgcagcaatt
    15721 ccaggtattc atcttcgcca attgcttcca tccctgatgc aatggcttcc ggatcgaccc
    15781 tcttctgtct cagtgccatg ctgatcttta cccgtcccca ctggctgtgc ctgaaatggt
    15841 cgaccacgaa tgccctgctg tagcgttgtt cgtcgatgaa cttttccttt atcagccttt
    15901 ctatgatcca ggtgcgtgtc tcggcgccat ctgcgcccca gcggtcaagc agagccgcga
    15961 tgtcactgat gcagtattcc ctttccgagc aggctctcat tgccctctgc agcagttcct
    16021 ccctctgttt cttttcagcc atgtttctca gccttgataa atctttcctt ttccctgata
    16081 tctttcaaga cccggacctc cctgtaggtt tccggtgaca gcaatgcagc cgtctcccct
    16141 gcaagatttt cattcacctc gagccacagg gtgcctccgg gcagaaggcg tgtttcagcc
    16201 accgctgcta tggcccggta gtaaagcaac ggattgtcat caggcacaaa cagggcctca
    16261 tggggctcgt gatttagcac gttgccgtgc attgcagcct tttcctgtcc ggtgacatat
    16321 ggcggattgc tgacgattat gttgctcgcc gggacatggc ctggatcatc gttgaggatg
    16381 tcagcctgaa caagggtcac tgctgcacct gtcctggtga tattgcgtat ggccacattc
    16441 agtgccgcat ggtacaggtc cgtggccgtg atgtcagcat cggggaatgc cagcgacaga
    16501 gcaattgcta tgcatcctga gccggtgcag agatcggtga cggttccggc gaagccgctg
    16561 ttttcaccga tgatcatcat ggtcatttcc tcggtctcgg gtcttggtat aagaaccccc
    16621 ggtgccacct caatcctgtg tccgcagaat tccgtatggc ccaggatata ttgcagcggc
    16681 tcgccggctg cggcgcgggc cacggcattc tctatacggg cggcggcacc ggcctccgcg
    16741 acacgctccc cgaaagccat ttgcgccgcc ctgcccatcc cggtatactc ctgaagaatg
    16801 gctgacgcca gcgccgcggc ctcctccgca ggccatcccg ccttaagcaa ttcagtgatt
    16861 cggcggctga tttcgcttat tgtttgtaac tttccggcca tgattcaaag ataggaaatt
    16921 gatctcatga cccgacctga tgacctcata tggatgagac gcgccctgca gcttgcatca
    16981 atggctgagg gatgcacctc acccaacccg atggtgggtg cggtggtggt gcatgacagc
    17041 gtcataatcg gagaaggtta tcatctgaaa gctggcaccc cccatgcaga ggtgcatgcc
    17101 atcaacgccg tcagcgatcg cagcctgctg ccgtcctcaa ccatttatgt aagcctcgag
    17161 ccatgctcac accagggacg cactccccca tgcgctgacc tgataatcag cagcggcata
    17221 cgccgggtgg tcatcggcac ccctgacacc aatccgctgg tggcaggcag aggaattgcc
    17281 cgcctgagac aggcagggat tgaggtgatt accggagtgg cggaggagga gtgccgccgc
    17341 ctcaacagac gcttctttac atggcatgaa aaaaagagac catacgtgat actcaagtgg
    17401 gcccgcagtg cagacggatt catcgatctg cgcaggaagg cctctgacgc aatcggacca
    17461 aactggatca cggggatggc agaacgggtt ctcgtccacc gctggcgcgc tacggaagat
    17521 gctattctcg ctggcggagg aaccatccgc gctgacaatc catcgctcac agtcagatac
    17581 tggagcggcc ggagtccggt acgcatcatc gtcagccgca gcggcaacct tgatcctgac
    17641 tcttcagtct ttgacgggtc agcagaaaca gttctcttca cctgcaacgg gcaggcgcag
    17701 ttccctgaca ccagaaccgt cacactgacg ggcgctgaac cgtgcgtcag cgaggtgctg
    17761 gcaaacctgt ggcaggcagg catacagtca ctctttgttg aaggcggcgc cttcatcata
    17821 agcagtttcc ttgagtcagg gcaatgggat gaagcacggc gtttcaccgg taccgcaaac
    17881 ttcgtcgacg gggtgcctga tccgttcccc ggattcactc ctgcgaaaac agtgtttttt
    17941 gacaaaagta ttctggattt tgcgtatcat ttttgagtga attccgtata actgatgtta
    18001 ataactgctt ttatttgtta gtaaataata attcgtattt ttgagacccc aaccgggaaa
    18061 ggacctaaag tcatgaagcg aggattcata gttcttgtat tgttgcttgt tgtcacctgc
    18121 atgaatttag caggtcaggc ggcccgcaag tacatcaggg ccggtgagga gttcaccagg
    18181 aatggcatgc ttgaggatgc cattgagcag tactcgaagg ctattgacga ggatccctcc
    18241 tccgctgacg gttatgtcag acgggccagg gtgcatgaaa tgaccggcag ttacgggctt
    18301 gcctgcgatg attaccggag agcgaacaat tttctccccg atgatgcagg cattctccat
    18361 aacctggggc gcatgtgcaa catgcttggc gcctcggcaa aggcaactcc cgaagaaaaa
    18421 aacaaattct attcggaggc ggtctcttac cttcaggcgg ccataaggaa agaattccga
    18481 aacgggaaac tctataccga aaaggtggta tccctgatag ggatggagca atgggacagg
    18541 gctctgggtg catccgacac cgcaatacag atgcgcgatg atgcagtcaa ctattaccac
    18601 cagggcataa tctaccagag aaagggagat gacaacactg ccagacggca gctcgaaaaa
    18661 gcagttacca aagacaagtc attcgcagcc gcacgcctcg aactggccag ggtgctggtc
    18721 cgccagggag atgtaaacag cgccctcgga cagtgcaaca tggtgattca gcaggatcag
    18781 aagaatgttg atgcatatct tacccgcgcc atggtttatg aggcaaaact tgactatccc
    18841 tctgcaataa atgacgtctc caccgccata cttcttgaac cggccggaca gcagcactac
    18901 gtaacccgcg gcatatgcta ccagaaattc aaccagcatc ttgctgccgt cagtgacttc
    18961 acaagggcgc tgacaattga ccagttcacc ccggagatct acctcatgcg cgcccgctca
    19021 tacgaggaga tccatgactt tgaaaaggcc gctgacgatt ataccagggt ggtggcaatg
    19081 aatgagaaca gctcctcggc aaagaaactg ctcaccgtgg caaacgaccg gctctacgcc
    19141 atcaaccgtg aaaacaacag cccggaaata tggctcgaaa ccccgaccgt cactcccaaa
    19201 agcgagatcg agataggcct tgacaagaaa agcattatgg taaccggaaa ggtgacagac
    19261 aaaagcctcc tggccgggtt ctcaataaac aatatccccg tgaggtacga aggtaaaggg
    19321 actattccct ttgctgcaga ggtggagatc ccatctgatg aaacccttcg aatcgttgcc
    19381 cgtgatgtct atgacaacga aaaggtactg acctaccgta tcaggcgtac cgaggtcgac
    19441 cctccggaga tccagattct cgccccttac atcctgggcg aaaatgtgct tgttattgac
    19501 agtatcgcat caacgattga catcgagggc agggtgaagg accagaacct gatcaggtca
    19561 gtgaagatcg aaaacatcag cgcccagttc tcacaggata accttaatcc tgccttcatc
    19621 gccaagggaa tcgatgtaag aaacaatgac aaggttacca tactggtgga ggacgtgtac
    19681 ggcaaccgca ccatcatgga ctatgcgctt gaccgtaacg gcacaaccat ttccgcatca
    19741 aatcccatgg gaaataccct ggtggtcttc atagagaaca gtgattatca gaactttgct
    19801 gccctccagg ggccgcccaa ggacgccagc ctcattaccg gtgtgcttga ggatgattac
    19861 caggtgctgc ccgttaaacg catccgcaac cagaccaaac aggagatgga aaagttcttc
    19921 tccatcgacc tgcgtgacga ggtaagggca aaacagatca agtcactggt catctggttc
    19981 gccggacacg gcaagttcat caatgacgtg ggctactgga tcccggttga cgcccgtcgc
    20041 gacgaggagt ttacctactt cagcctcaat accctgaggg cgtctatgga aacatatatg
    20101 aactatctga cacacgtgct ggtgattaca gacgcctgcg aatcaggccc cagcttttac
    20161 caggccatga ggtcagatgc caggccacgc aactgtgccg actggagagc cacacagttc
    20221 aagtcgagcc agatcttctc atcagcaggt tacgaacttg cggttgacga ttcacagttc
    20281 acacgcacct ttgccagcac cctgcgcggc aacccgaaca catgcatctc catagacgag
    20341 attgttcttc acgttacaaa agccgttacc gggaacaacc agcagaagcc caggttcggc
    20401 aagataaccg gcctgaagga tgaagacggg acattcttct ttgtaaagaa ataacctgat
    20461 gcgagggcga ggcagcaact ccggcaaata ctcaattacc gtcctggcat tactgcttct
    20521 gtctcagctt atacttcctc cccggctata cagccagaca ttctattttg aacagtacgg
    20581 ctccaaggag gggctgagcg cctccaaggt ttatacaatc attcaggatg ccaacgaatt
    20641 catctggctt ggcaccggca gcggcgtcac ccgctttgac ggtctgcgct tcgaaaactt
    20701 ctcgacttct gacagccttt ccccaggagg tgtaaaaagc atctgcgagg acagtcgcgg
    20761 caggctctgg ctggggcata tcggaggagg tgtttcaata atagaagaca acataatcag
    20821 aagagcatca ttttccccat ccatcttcat cggagttgat tctgcccgta taagtgaccc
    20881 gctgaggccc attaacagcc tgacgggaga tgttaccgga atcacggaat atgacggctc
    20941 catatgggtc tcaacctccc ttggcggagc gctgaggctt gacatgcctg aacgtggtgt
    21001 cagcctgatg accggccagc agtatgtcgg ccgccaggga ctcagcaatg acgctttcgg
    21061 cctatatgcc gatcaccgcg aaaacctcta ctgcatcact gacatgggca tcaagaagta
    21121 tgaccatgca agtgacaggt tcattccttt caaccctgaa ggacttacac gctacttcct
    21181 gacaacaacc atattcgagg actcccgcgg caacatgtgg ttcggcacct tcaacggcgg
    21241 gctttaccgg cagaacggcg agacgggtga gatgggcttc ttcgatacac gcaacggcct
    21301 ctggggaaac ttcgtctcat acatccttga agactaccgc ggcaatatct gggtggcctc
    21361 aatggaagac tgggagagca ggggaggaat aacggttttt tcccccgagg gaacagttgt
    21421 ctacaacagc accaacggcc tgccggcact tcatatcctc tgcatgatcg aggacaagga
    21481 aaagaacatc ctgatagccg atcgcgatgc gggccttttc atctacaagg gcgaccactt
    21541 cgcctcgttc atggcgccgc agttcatgcc gtcaaatgac gtctttaccc tggcccagga
    21601 tcacacaggc aggtactggt tcggcaccgg caggggcctt tcactctaca accccgccct
    21661 gaaagaggag gaccgtatca agctcagcgg atttccgccg tcgctcgagg ggacacagat
    21721 aagcatcatc aggcctgaca atgaggggca cgtatggatc gccacggcag gtgacgaagt
    21781 ataccgctat gatatctacg agaagaattt ccggtatgac cttgacctca acgccagcct
    21841 ccgatcgctg agaatcgctg ccatgaccac tgacctgagg gacaacctct ggatcggcac
    21901 tgaccgggcc ctcatcatgt ggaatccgca actcaagcag agtacgacat acacacagat
    21961 caacggcctg acgggaaata atatcacagc cctcttctgc gacagccggg gcttactctg
    22021 gattggcacc gaggaacgca acggccttac caggttcaac cccggaaaca atacgttcac
    22081 ccccctgacc ttcaggtccg atgtgcatcc gacagccatc accgagaccc ctgacggaat
    22141 gatctgggtg gggacaattg acggactata cagcctgagc aatgacacgg tgaaaaagaa
    22201 actcaccgag gctgaagggc tgctgatgaa ttcaatcaac ctgcttcagc ctgacaggtc
    22261 cggtaacctg ttcataggca ccaacctcgg gctgaacaga tatgacagca ataccggccg
    22321 gatctcatca ttcaccgaaa agagcggatt caccggaatt gagacaaggc ccaatgcttc
    22381 attcgccgat gacatgggtg atctctggtt cggcacttcc aacggggcca tgaggctctc
    22441 tccgtccatg ttgcctccgg tggtgacaga accggcaaca cacatatgga gaaccctggt
    22501 caactatgag gtcttcccca tgcatgacgg cataaggctc ctgcacaccc agaacaggat
    22561 aatttttgac ttcctgagca tctgccttgt taatccgggc gccgtgagat accaatttat
    22621 gcttgagggc gcagacaagg attggatcac cactgaccag aacagggcag cctactcgtc
    22681 actcagcccc gggaagtata ccttcctggt tagagcctca aacaattacg gttactggaa
    22741 cgaggtgccg gcaaaactgt cattctccat cagggggccg gtatacatga catcatggtt
    22801 catcatactc tgcatcaccg ccctggtggc gggaatttat ctgtttataa gcctccgcga
    22861 gagaaccctc cggcgggaga aggagcacct ggagcagaag gttgaggagc gcactgccga
    22921 agtggtcagc aaaagcctgg agctggagga gaagaacagg gacaccaccg ccagcatcag
    22981 gtatgcagag agaatccagg cagccatgct gcccccggaa aatttcttca atgatacctt
    23041 cgtgctgttc atgccgagag acattgtcag cggtgatttc tactggatgc atgacagcgg
    23101 cttcaaacag tatattgctg ctgtcgactg caccggccac ggcgttcccg gcgcctttat
    23161 gtcaataata ggccacaact ccctcaacaa gattgtcagg gagagtggca taacaaaacc
    23221 ttctgacatc cttgaccacc tcaacatcga ggtggtccat gccctgctgc agcgcagcga
    23281 aaaggccgtc agagacggga tggatatctc ggttatctca tatgacatcc tcgaacatac
    23341 cgttgagtat gccggggcgt accagcccct gtatcatgtc cgtgacggca aggttaccat
    23401 ttacaggggt gaccgcttcc cgataggaat gattgacggc caggccagga agagctttac
    23461 caacaacagg gtggacgtga agcccggcga tatgctttat atcttcactg acggctttgc
    23521 cgatcagttc ggcggggagc atgagaaaaa gttcaagacc ataaatatca ggtcgctgct
    23581 ggctgaaatc catatgcttc ccgttgagga acagaagcgc cgtctcgaaa aagccatcag
    23641 ggagtggatg ggcggactgg cacaggtgga tgatatcctg tttataggaa cccgggtgcc
    23701 ttagatcggg tcagccctgc agatacgttc aatgactccg gttcagatcc tggcaatcac
    23761 cttcgggtct gcatgccacc ctgtgtaatt gacaatggcc ggcacaacct cttcggcagt
    23821 ggtgactatc tgccagatat ccctgtgttc cagcctcatg aagtgacccc tgaccatgtg
    23881 gtcaaagaac tcgatgaggt ggtcatagaa gccgttgaca ttaatgatta caataggttt
    23941 attgaactga ccgagctgct tcagtgtgat cacctcggtg agttcttcca gtgtgcccac
    24001 gccacccgga agtgccacgg cagcatcgga catggcaaag atggtcttct tcctctctga
    24061 catgtcaggg gtgacgatca tatcacttac accgctgtga ccccatccct catccatcat
    24121 gaaacccggg atgacaccgg tgaccgaacc gccatgagcc atcagcgaat cggcaaagat
    24181 ccccatcagt cctatgccgc ctccgccgaa cacggcatgt acccggtgcc cggcaaatgc
    24241 cacgcctagt tcttcggcag ccttcatgta ggcagcgtca atacggctgc tggaggctgc
    24301 aaatacacat accttcatac tgttatcaga gcaggggagt gagtttggcc tcgaaggcgg
    24361 agcgtgcagc ggcgcccacc tgcttgtcaa ccacctcgcc gttcttgaaa taaagcaccg
    24421 tcgggatatt tcttatcatg aagcggcggg caacatccgg tgaactgtca acgtcacact
    24481 taacgaccag cgccttctcc ctgtaagcct ctgctatctg ttcaatgtac ggagctatca
    24541 ttctgcaggg tccgcaccat tcagcccaga aatcaacaat aaccggtttg tctgacttca
    24601 atacaacttc atcaaaattg ctgtcgttaa cttgttgtgc cataatgtta tatgtttctt
    24661 taattgctat ttccagttcc aaagatacct aatttacttt aaacccgagt tcgggattat
    24721 cttcaagcca tccgataagt tcatcggtga tacctgcctt tatgcttcgc gagaagagcg
    24781 ggattgtaat cctttcgtca tggtcaatca acaggaattt aagggatttg ttccccgggt
    24841 tttccgaaat aactgacttc aggtttttaa tgaattcggg attaaccagt tccggtttaa
    24901 gttttattgt aattgaagtt atcaggtctt ccctgacggc tgtcagaagg tggatctcct
    24961 ttattttgaa ttcaagttcc tcgggattga aatgtcttcc ctgcaccctt ccctttacaa
    25021 ggagctggta gtcgttcctg aaatagttgc tgaaggcgac ataatccttg tcgaacagca
    25081 ggaaggagaa tgagtccgta taatcctgga gggtgaatcc cccgaagggt ttcccgtttc
    25141 tggtcacacc gctgcgggtg tcgctgacga ttcctgcaac gcagacttca cggttggcga
    25201 actcactcag gttctggagg tctgccaggg ttgcattgca gaatgtgctg atctccagtc
    25261 tgaagtcgtc aagagggtgt gatgagaggt agataccgat aacctccttc tccttgttca
    25321 gtctttcaag cttggaccag tcggggcatg gtgccggttc ggggcgcacc aggtcaaatc
    25381 cgccggtgtc gccgaagaga gactgctggg tgctgttcct gatgttcttg acgttattgc
    25441 cgaagcggat gaggctttcg aggaagctgg agcctttgcc gtcagcggcg aagtactggg
    25501 ccctggtcat tcccggcagc ccgtcgaggg cgcctgatac caccatggct tcaagcattt
    25561 tcttgttgac ggtattcagg ttcacccttt ctgcaagatc atatatgtct ttgtatggac
    25621 cgttcttttc ccgttcctcg atcaggtgaa gcactgcatt ctcccccact cccttgatgg
    25681 cgccaagccc gaacctgatg ttgccgttac ggttgacggt aaactgcagg tagctctcat
    25741 tgacgtcggg gccaagaact tctgttccca tgcgcctggt ctcctccatc atgacggtga
    25801 tcttctttat gtcactgatg tttcggctca gaactgccgc catgaactct gccgggtagt
    25861 gtgccttgag gtatcccgtc tggtaagcta tcagagcata acaggttgaa tgtgacttgt
    25921 taaaggcata ttcggcgaat gattcccagt ctgaccatat tttattaatg gtgttctggt
    25981 catagccatt catgcggcag ccctcaatga actgctcctt gagcctgtcc atgatgtcgc
    26041 gcttcttctt gcccatcgcc ttgcgcagag agtcggcctg gcctttggaa aaacctccga
    26101 gttcctgtga gagaagcatc acctgctcct ggtagacagt gatgccgtag gtctccttca
    26161 ggtacttctc catggccggc acctcgtatg agatcttttc cttcccgtgt ttgcgcctga
    26221 tgaactgtgg tatgtagtcc atagggcccg ggcggtagag agcgttcatg gcgatgaggt
    26281 caccgaattg gtttggtttc agctcacgga gatacctctt catgcccacg gattcaaact
    26341 ggaatacgcc tgttgtctcg ccgttgctga agagttcata ggtgatccgg tcgtcgagtt
    26401 ccagggtctg aaggtcaatg gtgatgcctt ttgacttttc tatattgtca acggcatcct
    26461 tcatgatcga aagggttttc agacccagaa agtccatttt cagaagtccc accgactcaa
    26521 cgtggttgcc ttcgtactgg gtaacataaa ggtctgtatc ctttgcggtg cagatgggta
    26581 tgtgttctga caggctgtcc tttcctatga tgattccgca ggcgtgcaca ccggtctgcc
    26641 tgatgcttcc ctcgagcact tcggcatact tcagtgtctg ggccaccagc ggctcctttg
    26701 attccttctc cttcagcagt tcaggcactt cctcgtatgc cgatgccagt gttgtgcctg
    26761 gtctgtcagg caccagtttc gtcagcctga tggcttccgg caggggcagc ttctgcaccc
    26821 gggcaacgtc cctgatggcc atcttggcgg ccatggtgcc gaaggtaatg atatgtgcca
    26881 cccggtcatg accgtacttc tcaaccacgt acctgagaac cttctcacgg ccgtcttcgt
    26941 cgaagtcgat atctatgtca ggcatggaga tacggtcagg attgaggaat ctctcgaaga
    27001 ggagattata ttttacgggg tctatgtcgg ttatccggag tgaatatgca accacgcttc
    27061 cggctgcaga acctctcccg ggaccgaccc atacacccat ctcgcgggca gccctcagga
    27121 aatcctgcac tatcaggaag tagccgggga agcccatgtt tttaattacc gacagttcaa
    27181 agtcaatgcg tgcctgtata tcgggtgtga tggtgttcca ccgctcagct gcccctttca
    27241 ttgtcaggtg ccggagatac tcgtccttgt cggcaaattc cccggggatt gtgaattccg
    27301 gcattatggg ctcgctgttg agcttatagt tttcaactct gtccgcgagt tccaccgtgt
    27361 tgctgaccgc ttcggggata tcggcaaaga tttcgagcat ctcttctgcg ctcttcaggt
    27421 attcctgttt ggtgtaacga agtctcttct ggtcgtcgat gtccttgccg gtattcaggc
    27481 agatgaggcg gtcatgggct tcggcatcgt ccttcatgat aaagtggacg tcattcgagg
    27541 cgatgatttt gatgttgtgt tttgcagcca gctcacggta agcggctatc accctttgct
    27601 gctgggggaa gacggtgacg tctgatgtcg gatcagttgt ttcatggcgc tgtatctcca
    27661 gaaagtaatc atcgccgaaa atctccctgt aggaaaggat ggattcctcc gctgcgcgta
    27721 tgttcccggt cagtatctcc tgggccgctt cacctgctat gcaggcggat gaagctataa
    27781 gtccctcacg gtgtttgcgg agaagctcct tgtcaatgcg gggcttgtaa tagaagcctc
    27841 ttatccacgc ttcggagacc agcctgacaa ggttacggta gccggttttg ttttttgcca
    27901 gtaatataag gtggtcaccg gagcggtcct ctaccgatgt cttgtcttca aggcttgtcc
    27961 gggccacata gatctcgcat cctataatgg gctttattga tgcagcggta gcggcgtcat
    28021 ggaactcttt cacgccgaac atatttccgt ggtcagtgat ggcaagagcc gtcattccca
    28081 gttccttagc cctgcggacc agagttttta cggctgatgc cccgtcaaga atggaatatt
    28141 gtgtatgtac gtgtaaatga acaaataaag acataaaaac tgattgttac ctgatttttc
    28201 ctcgcctgct gacaaattta aggaaaatga gaatggtttt tgacaatttt gtgatatgga
    28261 attttttttt ctattacctt cacgcactta ttaggaaact acaagtcatc atttaattat
    28321 ttattaacta atctagaagt tatgaaaaga ttgctttcag tatttttctt gctttgtgga
    28381 ttactgctga cttctgcggc acagcagaag acagtaaccg gaacggttac ggcttccgag
    28441 gacaaccttg gaataccggg tgtgaccgtt caggtaaaag gaaccacatt gggagtagtg
    28501 accgacattg acggccgtta ctcaattcag gcctcacagg gacaggttct tgtctacagg
    28561 tttacgggcc agaagacaca ggaggttaca gtaggtgccg gcaacacaat tgatgttgtc
    28621 atgttgcctg accttctgaa cctcgaggag gtgatcgtgg tggcatacgg cgtacagaag
    28681 cgcgaggcca gcacgggatc agttggtgag gtaaaaagct ccgagataca gggcattccg
    28741 gaaacaagta tcgagaagat gcttaccgga aaggttgccg gtgtgcaggt tactgcttca
    28801 tcaggccagc cgggtgcttc aacacagatc cgtatccgtg gtatcagctc aatcaatgca
    28861 ggtactgaac ctctgtatgt aatagacggt atccccgtaa tgtcaggtga ccagagttat
    28921 tatacaaaca ccggtaatgc cctggcatca ctgaacccga atgacgttga atcaatgacg
    28981 atcctcaagg acgctgctgc ctcaagcatc tacggttcaa gggctgccaa cggcgtcatc
    29041 cttattacca ccaagtcagg taaggccggc gcttcaaaaa tcaacttccg tgcatcatat
    29101 ggaattgaac agctggctaa tgacaacggc tacaggcctc tcaccgctga ggagtatctt
    29161 acttaccaga ggacagctgt tatcaatgca ggcggtaatc ctgatgatcc gaccaacgca
    29221 aaatactatt tcccgatgtc actccttaat ggtccgatca ccgactggaa gaaggagctt
    29281 acacgcactg gaagcatcta ctcggcagaa ctgagcgccc agggaggcag tgacaagaca
    29341 cagcatttct tctcggctct gtatgacaaa tatgaaggaa ttgtttacgg atcagatttc
    29401 accaagtttt caatccgttc aaacgttgat catcagctga acaagaaact gaagcttggc
    29461 gtccgcttta acggagcata ttcacatgca aatgacgtgg ccatgcagtc actgtattat
    29521 gtgaacccga tgtttgcaat gattatgatc aagcccttca ctcccctgag gaatgaggac
    29581 ggttcatata acctgaacat cccggagaac gccaacagca acccgcgtgc aaccgcacaa
    29641 gatgactcgc agtgggaaaa gcagtacagg ctcaatacaa acggttacct tgagtaccag
    29701 atcgccaagg gattgaccgc caagacaacc aacagtattg aaatgactga cggtgagggc
    29761 agaaggtact ggagccctga ggctaactat ggagaaacga tgggttatct gcagtcatcc
    29821 aagacgaaat acgttctgct caccacctca aacacactta acttcaacaa ggagttcggt
    29881 tcacacaccg tgtcggtggt tggtggccag gaagcctcaa agttcgacta caatgagtac
    29941 tcaattatct ctccgaatgt tgacccgaac atcccgttcc ccaatacatc cacgtcggat
    30001 gacgatgacg ctgattatta tgaatcagct tacacaatct tgtcattctt cggtgtagcc
    30061 aactataatt tcgacaacaa atactacctg caggcaagtc tcaggactga cggatcatcg
    30121 cgcttcggct cgaagaaccg ctggggtaca ttctggtctg ttggcggatc atggaacgtt
    30181 cacaatgagg atttcatgga taacgttgat tggatcaaca tgctgaaggt tcgtggaagc
    30241 tatggtataa gtggtaactt caacataggg aactatgacc agtatggtct gtacggagca
    30301 ggacagtaca acggcagcgc cgtgatggta ccaacccagc cgtctaatcc tgacatcgga
    30361 tgggaaaata atagtgaata caacgccggt tttgactttg cactgtttga ccgtatcacc
    30421 ggttcattcg atgtttactc gcgcaagacc cttgacatgc ttcttggata tccgctctca
    30481 cgcacttcgg gtttcagctc gataaccacc aacatcgggt caatcaagaa cgagggtatt
    30541 gaattcatgc ttgacggaac acttatcgcg acagataaat ttgaatggac ggccggattc
    30601 aacatagccc acaacaacag taccattctc gaccttggca aggatgagca attcgtcaac
    30661 agtaacaaca cccgtatcct tcataaggtt ggtgagcatc tttacagctt ctacctgttt
    30721 gattatgcag gtgtcaaccc ggccaacgga gaagccatgt ggtacaacgc cgacggtgag
    30781 ctcacaaata agtattcaga ggcaagaagg gtcatcgcag gatctcccga acccaaactg
    30841 aacggcggta tcaacaccaa gctgacagca tatggttttt cacttgatct gaaccttgag
    30901 tataagtacg gtaacctgat atgtttaacc gagaaccgct attccaactc tgacggttac
    30961 agctggccaa acaactatgg caatactcag cttgattact ggacaacacc cggccagatt
    31021 gcaaggaacc cgaaacccgt tgctaacaac gcctcttctt caagtgcctc tctttc
//
LOCUS       DAOPXU010000025        30912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_1413_length_30912_cov_11.048417, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000025 DAOPXU010000000
VERSION     DAOPXU010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 30912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30912
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1413_length_30912_cov_11.048417"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="PKK03_04030"
     CDS             <1..707
                     /locus_tag="PKK03_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNX83596.1"
                     /translation="RSTLIRMPATVSEDVLLGKVRELNGDPELDGLIVQLPLPDHISE
                     DKVIETISPDKDVDGFHPVNVGRMAAGLPSFVSATPAGIMELLKRYNIPLKGAECVVV
                     GRSNIVGRPVSILLSQKGADATVTVVHSRTRNIAEIVRRADIIIAAIGQPGFITADMV
                     KEGAVVIDVGTTRVDAPDTKAGWRLKGDVDFEKVAPRCSWITPVPGGVGPMTRVSLLL
                     NTLQACRAQGKLMPVK"
     gene            complement(680..1675)
                     /locus_tag="PKK03_04035"
     CDS             complement(680..1675)
                     /locus_tag="PKK03_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83597.1"
                     /translation="MTSSRLLFFLAVLSIVPFFTGCREEPLKINYELGIFPDSVIALE
                     GLNTEHDDYNMDIEAGRIDARLPVVFSSNRQSAGGEFDLTHGLIWYSFGQTTGSFQLE
                     SEMAADVFLDRLTSAFNTGGNEFGPLRFFNGRNGLEYMAAATETDENGLDIVFTSYIP
                     AFTNLPAVEPPVGATLFNSQSDDAYLSLGTTLDTAWFCSDRGGDFDIYMLTRPAGLSL
                     GEWLMSPAATPALVDSINSTWDDKCPFVRGRYMVFASEMQGGMGGFDLYYSVFRNGKW
                     SSPVNMGPGINSAANEYRPVLGIDLKFENFFLIFSSDRSGGKGGYDLYFTGISLP"
     gene            1984..3390
                     /locus_tag="PKK03_04040"
     CDS             1984..3390
                     /locus_tag="PKK03_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX83598.1"
                     /translation="MEEERSGFVHDSELDEVILRFERMRKNKETCYFDVNEFETIIDY
                     YLEGNNPVHAFEATSLACELHPNSVPILIRKARVLLEKGRATEALTIARRLENIEPGN
                     CDIYLCKATALAMLGDINGARKVFDLALETDSDDLENILYSITSVLQNLNFHEEMIPY
                     LNKLAELEPDFPAHLYDLAYAYDKKGDQRTSIKYYNLYLDEDPYSDSAWYNLGIIYNK
                     TGDYGKSLEAYDYALALNPENTFALFNKANILSNLGKYSEAMDVYRQFVEEEPDSLEG
                     LTYLAECYDKTGDEAMARKYYAEAIDITPDFPDPWYGLGLLSLSRNRPAESITPLKKA
                     IELDPENPDYWFSLGKAHIMNGNIREAVRCYIEALIIDGYYDEAWIETGIVVMMTGAY
                     RGAIRILKKAAKISGDLPGIYFLLASAYLHTGNMKEATRNLVRAIRLDNELLEEYSVL
                     LPEEKLTNAMRRLFRKTN"
     gene            3394..4173
                     /locus_tag="PKK03_04045"
     CDS             3394..4173
                     /locus_tag="PKK03_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HNX83599.1"
                     /translation="MKQARELLTHIPVRPGKPRKEGLTMVMDKGLSLREAEDFAGVGS
                     EYVDFVKLGFGTSVITPNVDKKIKTYHEAGMTVYFGGTLFEAFIIRGLFAEYVDLLKH
                     AGVRMVEISDGSYEFDHSEKLQYIRKLSKDFTVISEVGSKKKNIIYTPAQWVEMMKAE
                     LDAGSVKVIAEARESGTIGIYNDDGSVNKDLIGTISREIGFDNVLWEAPLKQQQAWFI
                     AHFGANVNLGNIAPNEIIALESLRLGLRSDTFFSFLPDHMK"
     gene            4170..4919
                     /locus_tag="PKK03_04050"
     CDS             4170..4919
                     /locus_tag="PKK03_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNX83600.1"
                     /translation="MRLYGIIGFPLGHSFSQKHFSEKFEREHLSDHAYRKFELPEIGM
                     LPELLVREPDLCGFNVTIPWKVKVMDFLDDIDPVAAEIGAVNTVKVTRHGGKAWLKGF
                     NTDAPAFRESLLSNLDTLPDSALVLGTGGAAKSVIHTLIDLNIIAVPVSRKPVRGGYT
                     YDDLPGYMISEAGVIINTTPVGMAPHTEAMPPIDYSCLREGQLLFDLIYNPPVTAFLS
                     RGEEHGCITVNGEEMFLIQAGLAWEIWNDVE"
     gene            complement(4995..5747)
                     /gene="truA"
                     /locus_tag="PKK03_04055"
     CDS             complement(4995..5747)
                     /gene="truA"
                     /locus_tag="PKK03_04055"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNX83601.1"
                     /translation="MTRYFIFLSFRGTAYNGWQIQPGKHTVQGVLTKALSTVLSSQIA
                     VTGAGRTDTGVHASFFCAHFDAVRNDLNNDGQLLYNLNGILPDDIAIKRIVKVNPDAN
                     ARFDAISRTYQYTVTRVKDPFCADTAWLLYWQLDIDRMNEAAAILLKHSDFTSFCRLH
                     GSNRTNICRVTHARWTDEQEKLIFTITADRFLRNMVRAVVGTLIPAGRGKLALNDFDA
                     ILRGKNRGLAGQSAPAHGLSLTAIGYPGNIFI"
     gene            5836..6885
                     /locus_tag="PKK03_04060"
     CDS             5836..6885
                     /locus_tag="PKK03_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HNX83602.1"
                     /translation="MSDPELKRGFASDNNSGVHPAVMKELLRVNRGHVVGYGDDPFTE
                     TAMTLIKKELGAETEVYFVFTGTGANVLGLAGVMKSWNSVITAHTAHIETDECGAPEK
                     FTGCKVLSVDTPDGKLRPEMLARHMHGFDFEHHSQPGVISISQATEMGTVYTPGEVKE
                     LADYAHEHGLLLHMDGARIANAAVTLSVPFRAFTTDAGVDILSFGGTKNGMMYGEAVC
                     FLKPGLSEGFKYLRKQGMQLASKMRFISAQYIAYLSGGLWKECASHSNRMARELQRRL
                     KEISGIKITQKVEANGVFVIMPDRVAALLSREYFFYPWNEQTSEYRLMTSWDTTSDDV
                     VQFADRLKNMMSDYK"
     gene            complement(6950..8167)
                     /locus_tag="PKK03_04065"
     CDS             complement(6950..8167)
                     /locus_tag="PKK03_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNX83603.1"
                     /translation="MKMSYGDIKIADYTYDLPPERIAKYPLPERDKSKLLVWDGNISD
                     HIFSELPALTDHASLMVFNNTRVIRARLIFRKASGAIIEVFCLEPHSPAEFSMNLASG
                     GPVEWKCLVGNLKRWKSGMLESRFIHDGKEHILSAERTGTAGDAFTVRFSWNEAGLSF
                     AGVLECLGHMPIPPYLDRDDEESDVSAYQTTYARINGSVAAPTAGLHFTPSVLAGLDA
                     AGIPRAEVTLHVSAGTFRPVKSELICDHMMHSEHFFVSKQTLERLRSRKTTAVGTTTV
                     RTLESLYWLGMSLYEGREFKDRIPSVSQWEPYEKEGELDPAVAIDCLLAELERSGRDT
                     LEARTDIIIVPGYRFRMVSSMVTNFHQPHSTLLLLVAAFAGENWKKIYNHALASGYRF
                     LSYGDSMLLAPRT"
     gene            8217..8528
                     /locus_tag="PKK03_04070"
     CDS             8217..8528
                     /locus_tag="PKK03_04070"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNX83604.1"
                     /translation="MKGSRSSRPPSGGSKVREIDLHLRPGQAGVEALERQLARVRGEL
                     NSSIKAGAREIVFIHGSGSGKLREALRAAIAAEYPSCTCHDAPFARYGYNGATIVTIR
                     K"
     gene            8735..9110
                     /gene="rnpB"
                     /locus_tag="PKK03_04075"
     ncRNA           8735..9110
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKK03_04075"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            9106..9651
                     /locus_tag="PKK03_04080"
     CDS             9106..9651
                     /locus_tag="PKK03_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HNX83605.1"
                     /translation="MIQEMPPGVGGIFCVCPALAITMRSAHQALSKCRDYITEGYVYA
                     VDIDLERFFDTVNHGKLIEVLSRKVKDGRVVSLIHKYLNAGVMKEGRYEETPAGVPQG
                     GPLSPVLSNILLNELDIELERRGHKFVRYADDMVILCRSRRSTERTMQNIISFIEGKL
                     LLKVNRDKSKAAYVREVKFLG"
     gene            9712..10104
                     /locus_tag="PKK03_04085"
     CDS             9712..10104
                     /locus_tag="PKK03_04085"
                     /inference="COORDINATES: protein motif:HMM:NF019984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HNX83606.1"
                     /translation="MKTRIKELTSRSNGWGNDRRKEALSQYIKGWLQYFRLADMKTLL
                     EGIDEWYRRRLRMVIWKQWKRIRNRSSNLKRLGISKSQAYMWANTRKGYWRIAGSYIL
                     STTITSVRLRLSGYVFFTDYYSKVRVVS"
     gene            complement(10269..10970)
                     /locus_tag="PKK03_04090"
     CDS             complement(10269..10970)
                     /locus_tag="PKK03_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83607.1"
                     /translation="MKKSMTLLAAFLVASVCSAQSLDEIANKYYAANGIENLEKAQTM
                     TMKGVVSQMGMELPITIMVKKPNKVKVIQEFNGMEIVIAFDGEKGFMLNPLTGATEAV
                     ELPAEQLGQVQEYNVFKDSFMEAFKAGRMKLEGEEAVEGKPAYKITVTSESGATSTTY
                     LDKESFLPVKTAQTVSQMGQEMLVESYIRERKEINGVKFGTKIAQFVEGSELGGMTFQ
                     TIEFDTPMEDSIFKL"
     gene            complement(10992..11819)
                     /gene="kdsA"
                     /locus_tag="PKK03_04095"
     CDS             complement(10992..11819)
                     /gene="kdsA"
                     /locus_tag="PKK03_04095"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNX83608.1"
                     /translation="MKVSELNIPLLKHTGSGNFLLLAGPCVVESEEMTLRVAAEVVKL
                     SDKYHIPLIFKSSYRKANRSRLDSFSGIGDEKALEILAEVREKYSVPVVTDIHSPEEA
                     RIAAHFVDVIQIPAFLCRQTDLLVAAAATGKVVNVKKGQFLSGASMRFAAGKIRESGN
                     ERVMLTERGSFFGYNDLVVDMRNIPVMKQIGAPVIMDITHSLQQPNQEGGVSGGQPDM
                     IETIGRCAIAAGVDGIFIETHPEPASALSDGANMLRLDMLDSLLYKLTLIRTTVNSF"
     gene            complement(11841..13145)
                     /locus_tag="PKK03_04100"
     CDS             complement(11841..13145)
                     /locus_tag="PKK03_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNX83609.1"
                     /translation="MFSNQPLADRLRPRSLEEFAGQEHLVGSEGVLRKVIESGNVPSF
                     ILWGPPGVGKTTLARIVANKLERSFFQLSAVHSGVKEVRETIEKAKSQQFFGKPNPIL
                     FIDEIHRFNKSQQDSLLGAVEQGILTLIGATTENPSFEVISPLLSRCQVYVLKPLDDE
                     TLRKLIDRAVTDDYYLSRYKIRVAEYEALIRISGGDARKLYNALELTVSLEAEKGSGE
                     IVVTDEMVLGHVQQNLAMYDKNGEQHYDIISAFIKSLRGSDPNAAVYWLARMVEGGED
                     VKFIARRMVILAAEDIGLANPNALLLAQACFNAVNLVGWPESRIILSEAAVYLATSPK
                     SNASYMAIENAIAAVRQHGDLPVPLHLRNAPTRLMKNLGYHEGYRYAHSFEDNFVQDN
                     FLPEQITGTVFYDPGRNARELEIRKQLETRWQKIYRYKKDNE"
     gene            13338..14498
                     /locus_tag="PKK03_04105"
     CDS             13338..14498
                     /locus_tag="PKK03_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83610.1"
                     /translation="MKKPLLITLLVLAVLLLIPAVSFIRWAFQEKKPVDVVILDKTVP
                     TLDRLGHRSFVYILTNARYVRGEKGGSYSANKDYYGFEPLRPVREKQFRKRDFRLTEL
                     IDLADKNDVLYYTDTYGVFFNDWYQGIKKTRRSRKLYGGLNNNDYLLMVEMKKRDKLV
                     LVEYNTFDYPTAGLERFKTEELLGIASKGWMGQYYKSLDTVSQQGVPAWMPALYRKQY
                     REPWTFTKPGVVLLRENGIIVLEEGKHLASALPVIRTTGPSAERFGLPETVAFTNSFD
                     IIDPGRNNVVSEFVLNTTPSGDTLLAVNDLQAVFPAVTQEPLTQKTYYFSGDFANNNI
                     PFWTARIKNIQKGAKSFLYSEKPDDPKRFFWLYYKPLITSIFAEYQAEHAGK"
     gene            14641..15408
                     /locus_tag="PKK03_04110"
     CDS             14641..15408
                     /locus_tag="PKK03_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007173866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNX83611.1"
                     /translation="MKATIVQCGIKWEDRDSSLAHISALLDAAPPGGGIVVLPEMFST
                     GFTMNPAPLAEGMDGPTVRWMKERAANGGYALCGSVIIGEEDRYYNRLIFVTPVGDVT
                     FYDKRHLHTMSGEHTVYSRGNKRVVVTYREFSFNLQVCYDLRFPVWSRNRGDTDVIIY
                     SANWPSVRSSVWKALLVARAIENQCYVIGVNRVGANPDGTSYTGDSAIVSPKGELMAS
                     LGPGAEGVVSAMLSREVLDKYREDMPIWRDADAFTIL"
     gene            complement(15475..18414)
                     /locus_tag="PKK03_04115"
     CDS             complement(15475..18414)
                     /locus_tag="PKK03_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNX83612.1"
                     /translation="MEDQRTATLTELLTSGFEGEVRTDRSSRLMYATDASIYREEPLA
                     IVFPRNTDDIKRLLAFAAKHRIGIIPRAAGTSLAGQVVGSGIVADISRHFGKILEINV
                     EERWVRLEPGVVPDELNAALRSHGLLFGPETSTSSRCNLGGMIGNNGCGLHSVVYGSV
                     RDHIIGVSALLHDGSEVVFGELTADQFSQKCSLQTAEGEIYREIEQIMTDPGHRESIM
                     AGYPEKTIPRRNTGYSLDDLLCSEPFTPGAGRKINLARLICGSEGTLAIVTEAKMGLV
                     PLPPPVKALACVHLHHRNEAFRANLIALRHQPWAVELMDSNILSLAATVESQKRNRFF
                     IEGDPGALLIVEFCAGSREEIIFTAAVMEEEMRSEGLGYAFPVVWGADVKRVWDLRKA
                     GLGILSNMPGDAKPISLIEDCAVDVNRLGDFVADIEEMLASYGKESVYHAHIGTGELH
                     VRPLINLKDAGEAKLLRTIAEETARIVKKYRGSMSGEHGDGRLRGEFIPLIIGAGNYK
                     LNKRIKRVFDPNGILNPGKITDTPPMDSSLRYIPGKPTPEPATHYDFSDCGGVVRAAE
                     KCNGSGDCRKSSVIGGLMCPSFMATGDERLTTRARANIMREMLYADRDNPWDSREIYE
                     ILDLCLACKGCRSECPSGVDIAKLKSEFLQHYNDVHPPSLRTRLIASLPKIYSLFSFI
                     PGIFNFFATNKFTALIIKRVTGFAAERSIPVLAPQTFRRWLKKNLSALNPANPIGEIC
                     LFVDEFTNHNDLDAGKAAAKLLTGLGYRITFAGNAASARTYISKGFLRQAKKLITKNI
                     LVLGPLVSDDCPLVGIEPSAILGFRDEFPDLAGDKLRPEASRLAQHTFTLEEFIAGEF
                     SAGRIKREMFTREPAEVLVHVHCQEKAITTSAPVLAALGIPVNYKVREIPSGCCGMAG
                     AFGYEKEHFELAQKVGELVLFPDVRSASAGTIICAPGTSCRHHVKDGTGRVALHPAEV
                     LLAALRG"
     gene            18601..19608
                     /gene="pta"
                     /locus_tag="PKK03_04120"
     CDS             18601..19608
                     /gene="pta"
                     /locus_tag="PKK03_04120"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HNX83613.1"
                     /translation="MELLEQIKTNARRHNKMIVLPEGTEERTLKAADAVLAEGIARIT
                     LLGNPAEIKTMAESFGLKNIGKAQIVDPHDHPAKKHYIDTMVELRKSKGLTVEEASRL
                     IEDPLYLGVMMIKCGDADGEVAGARNTTGDVLRPAFQIVKTAPGISVVSGAFIMMLKD
                     KEFGAEGIIVFADGAVHPDPTDKELAEIAVATARTTKTIVGVEPKVALLSFSTMGSAK
                     HPLVDKVVSATKMAKEMAPEFMFEGELQADAALIEAIGQKKAPGSRIAGKANVLVFPN
                     LECGNIAYKLVERLAHAQAIGPVLQGMAAPINDLSRGCSVSDIINVIAITANQAAGLT
                     R"
     gene            19622..20833
                     /locus_tag="PKK03_04125"
     CDS             19622..20833
                     /locus_tag="PKK03_04125"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNX83614.1"
                     /translation="MNILVLNCGSSSIKYQLFDMQDSARVLAKGLIERIGITDGILTH
                     KPTGKDPYKVVLDIPDHTVGINMVMDALVDPVHGVIKSISEIKAVGHRVAHGGENFKE
                     SVLIDNDVKRDIEKCAELAPLHNPAHLKGILSCEKLLPGIPQVAVFDTSFHQTMPDYA
                     FMYAIPYEYYEKYRIRKYGFHGTSHRFVAEKACKMLNMDLYNSKIITCHLGNGASITA
                     IENGKSIDTSMGFTPVDGLIMGTRTGEIDPGVLLYLADKEELSVSGVNNMINKKSGVA
                     GISQLSSDMRDLEAAANEGNPKALLALNMYAYKIKKFIGAYVAAMNGLDLLIFTAGVG
                     ENDFNARKMICTGMEYMGIEVDEDVNHGVKGKDLVISKPSSKVKVMVITTDEELVIAS
                     DTQAIVEKLTH"
     gene            20846..21754
                     /locus_tag="PKK03_04130"
     CDS             20846..21754
                     /locus_tag="PKK03_04130"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HNX83615.1"
                     /translation="MAFKHLSELKQLLVDKPARRLVLAAAQDHNSLSSVLMAAHDGFI
                     EPILVGDKDAIHNIAAQNNFDLTGVRVVHEPDSEMAIEIAVRMVRSNQADILMKGKVG
                     TSNLLKGVLNKEWGLRSGSLLSHFAIFEVAAYHKLIAVTDVAMNIAPNLQDKIAIVNN
                     SVACLNRLGVEMPKVAVLGAVEMVSENMQATLDAALLSKMNQRDQIRHCLIDGPLAFD
                     NAISLESATHKGIRSDVAGDTDLLLMPDIEVGNVLYKSLVFFAHAGVAAVILGATAPI
                     VLTSRSDSDQAKYYSIVLAAAITRKE"
     gene            21756..22670
                     /locus_tag="PKK03_04135"
     CDS             21756..22670
                     /locus_tag="PKK03_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNX83616.1"
                     /translation="MITSLRQMADTVRSSGTKKKIAVAWAQDPNTVGSLHRAVAEGFA
                     EAIMIGRRDRILATCQETGADPRLFTLVESPDEKSAAELAVSITRGGEADVVMKGLVG
                     TDKFLRAVMDREKGLLPPKAVMSYVCAVEIPAYHKLLFITDTAVIPFPDLDQKVAMAR
                     YAVDMARRFGIGKPRIALIGASEKVSDHFPNTLDYSVMCKMAERGQIPDCIMDGPVDL
                     FLACDPESVRIKGVDTPLAGDADILLFPSLEACNPFYKGLMLFGGGELAGMICGTSKP
                     VVLMSRSESELSKYYCVALACLMASQKL"
     gene            22676..23755
                     /gene="buk"
                     /locus_tag="PKK03_04140"
     CDS             22676..23755
                     /gene="buk"
                     /locus_tag="PKK03_04140"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNX83617.1"
                     /translation="MKEKLILAINPGSTSTKFALYEGKDKLMEKTLRHSTEEFEGFTT
                     MTDQLPFRYRVIMNALREEQADLKEIAAVVGRGGLVNPIESGIYEVNDLMKKHLSEGV
                     NGEHASNLGALLADMVVKELPGAKAYIVDPVVVDELEPIARLSGHPLISRRSIFHALN
                     QKAVARVYASSIGREYEDLNLIVAHMGGGISVGAHRRGRVIDVNNALNGDGPYSPERS
                     GGLPAWQLSELCFSGKYKPSEMKSMLAGKGGIVAYLGTNSFQDASKRATEGDTHAALV
                     VEGCSYQTAKEIGAMSAVLEGKIDCIILTGGLAYDSAHVERVTRMVKHIAPVYVYPGE
                     DELKALAFNGYLAITGHIIVKEYRG"
     gene            complement(23835..24530)
                     /locus_tag="PKK03_04145"
     CDS             complement(23835..24530)
                     /locus_tag="PKK03_04145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX83618.1"
                     /translation="MNIVITGASSGIGRETAIRLSEEPGNKIYAIARNRKSLTGLSEA
                     AGHKNIIPVPMDITSGRSSLSELKKRLEREAGKVDILINNAGHLVNKPFEDHTEADIA
                     ESVSVNFTCAARLTAELLPLMVRGSHVVNIGSMGGFQGSLKFPGLSWYSATKGALAVL
                     TECLAAEYAERGISFNCLCPGAVQTEMFQKAFPGFSAPVLPSDMAAFIADFAVNGNRF
                     FNGKILPVAMSVP"
     gene            24640..25209
                     /locus_tag="PKK03_04150"
     CDS             24640..25209
                     /locus_tag="PKK03_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83619.1"
                     /translation="MKKILALAFFLVIAATAAGQIPATENAEEERPPVNQRLFFGGSF
                     GLQFGTVTNIEVAPLAGIWLLPRLAVGAGPSFQFYKDPYGRTSIYGGRAMLQLTLIQD
                     LNNVIPIGLNTGIFVNGEYEALSLQRAFFTSDPEADGRFYHGTFLAGAGLSQPTGRRS
                     SMNISFLWSVTGNEYGIYDTPEIRIEFYF"
     gene            25250..25786
                     /locus_tag="PKK03_04155"
     CDS             25250..25786
                     /locus_tag="PKK03_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX83620.1"
                     /translation="MKIENNKFASLAYTLHEGDKGGRVIETVEESAPLSFVFGAGRLL
                     PAFEEKLVGLEEGAAFDFSLAAADAYGEVREEMVISLPRNIFEDEGVLRSEICYVGNT
                     VPMMDSQGNRMNGVVVEIGDAFVKMDFNHPLAGTDLHFTGKVVGVREATPEELMGPSS
                     GGCSSCGSKSSGCGGSCG"
     gene            complement(25841..27076)
                     /locus_tag="PKK03_04160"
     CDS             complement(25841..27076)
                     /locus_tag="PKK03_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNX83621.1"
                     /translation="MVKRRFSLAMHWQILIMLALGGLFGYFFPHLTKYTNWIGDVFLR
                     GLNMVIVPLIFCSIMTGVASVGTAENLGRLGLKTMGYYVFSTFLAILTGFFFVQLIKP
                     GIGADLGLQVPLDEISTGKESFGQTLINIVPVNIIRAMSEAQMLSIIFFAILAGFFVT
                     RVGEKSRVLLVDVVNAILDVIMKITLFVIKFTPLGVFSIIAKVLSQQINSGNQVGEII
                     GSMGIYFLTVLLGLVVQGFVTLPLLVRFLGRANPVKHLKNMSTVLLTAFSTSSSNATL
                     PLTMETVEARNGVSNKIASFTLPLGATINMNGTALYECVAVMFIAQAYGIDLSFGQQM
                     VVIFTALLAAIGSAGIPMAGLVMMAVVLNAVGLPLEGIGLILAVDRILDMFRTAINVY
                     GDTCGAVIIAKSEGEELLV"
     gene            27734..28186
                     /locus_tag="PKK03_04165"
     CDS             27734..28186
                     /locus_tag="PKK03_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX83622.1"
                     /translation="MKDRIQAFLQNENKSYAQFADEIGVQPSGISHILSGRNNPSLDF
                     VIKMLHRYPSLSADWLLFGRGSMYKVVSQPTLFDVDMQKDQPAENEVFAEQDLAADNG
                     DIPDVNASRQDVAEVFQTEGNEPESGSRKLSRIVFFYSDKTFTEYKPS"
     gene            28231..29010
                     /locus_tag="PKK03_04170"
     CDS             28231..29010
                     /locus_tag="PKK03_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNX83623.1"
                     /translation="MKKQIEDLTVIGNSKLTEGFFVIKVSSSEDLTGIKPGQFVQALV
                     TESPSTFLRRPLSVHDIDPGENTMSLLVQIAGPGTERLSRITPGEKLNLIYPLGNSFT
                     LPARGERVLLAGGGCGMAPLLYLGRKISESGVQPHFALGFRNRSRILSHDEFSALGPV
                     FLATEDGSKGYRGFVTDIPEFRNEHWDRVYCCGPEPMMKAVAALCRKRDIFCEVSLEN
                     LMACGLGICLCCVVPTTSGNLCSCTDGPVFNINDLKWQTSG"
     gene            28992..29906
                     /locus_tag="PKK03_04175"
     CDS             28992..29906
                     /locus_tag="PKK03_04175"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNX83624.1"
                     /translation="MADLRIKIGQLEFRNPVILASGTCGYGSELEDFINLSELGGIIV
                     KGTTLEHREGNPYPRMSETASGMLNAVGLQNKGIDYFEKNIYPHLAEYKTHVIVNVNG
                     NTIEDYVDLSQRINTLSAIPAIELNISCPNVKKGGMIFGTNPASAREVIYAVRRVYDK
                     TLIVKLSPNVTDITEFARISQEEGAEAVSLVNTFLGMAVDVRTMRPKLSTITGGLSGP
                     AIKPVALRMVWQVARAVSIPVIGLGGIMNAEDALEFIMAGATAVQVGTASFINPRAGT
                     EIVGGIEKFLVEKEMDSVSNITGIINRI"
     gene            29978..30448
                     /locus_tag="PKK03_04180"
     CDS             29978..30448
                     /locus_tag="PKK03_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNX83625.1"
                     /translation="MAENLQIDNLDRKILDIITKNARIPYLEVARECNVSGAAIHQRV
                     QRLIRVGVIKGSEFKVDPIMVGFRTCAYIGLYLDNPGKYKEVISKLKDIAEVIECHYT
                     TGNYSLFIKVYTRDNEHLREVLTEKIQAITGVMRTETLISLEESISRQIPVLSK"
     assembly_gap    30726..30825
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7880 a         7585 c         8082 g         7265 t
ORIGIN      
        1 tcaggtccac actcatcagg atgcccgcaa ccgtgagtga ggatgttctg ctcggaaagg
       61 tcagggaact gaacggtgac ccggaactcg acggactgat cgtgcaatta ccgctgcctg
      121 accacatctc tgaagacaag gtaattgaga ccatcagccc ggataaggat gttgacggct
      181 tccacccggt gaatgtgggc cgtatggctg ccggactccc ttcttttgtc tcggcaacac
      241 ctgccggcat catggaactg ctgaaacggt acaacatccc tctgaagggc gctgagtgcg
      301 ttgtggttgg ccgcagcaac atcgtgggcc gcccggtcag catactcctg tctcagaagg
      361 gtgccgatgc aaccgtcacc gtggttcaca gccgcacaag aaacattgcg gagattgtca
      421 gaagggcaga tattataata gctgccatcg ggcagccagg attcataacc gctgacatgg
      481 taaaggaggg agcagtggtg attgatgttg gcacaacacg cgtagatgca cctgatacaa
      541 aagcaggatg gaggctgaaa ggcgatgttg attttgagaa ggtcgcaccc cggtgctcct
      601 ggataacccc ggttcccgga ggcgtcggtc ccatgaccag ggtctccctg ctgcttaaca
      661 ccctgcaggc atgcagggct cagggcaaac ttatgcccgt gaaatagaga tcgtatcctc
      721 ccttgccgcc tgatctgtca gacgagaaaa tcaggaaaaa gttctcgaac ttaaggtcta
      781 ttccaagcac cggcctgtat tcgtttgcgg cggagttgat cccgggcccc atattaaccg
      841 gggaactcca cttcccgttg cggaagacgg agtagtacag gtcaaacccg cccataccgc
      901 cctgcatctc agaggcaaac accatgtacc tgcccctgac aaaggggcat ttgtcgtccc
      961 atgtgctgtt aatgctgtca accagtgcgg gtgtcgccgc cggtgacatc aaccattcac
     1021 cgaggctcag cccggcaggc ctggttagca tgtaaatatc aaaatctccg cctctgtcag
     1081 aacagaacca tgctgtgtca agcgtggttc ccagggaaag ataggcgtca tcagactggc
     1141 tgttgaagag tgttgccccg acagggggtt cgaccgcagg caggttggtg aaagcaggga
     1201 tataacttgt aaagacgatg tccagcccgt tttcatcagt ctccgttgcg gccgccatgt
     1261 actcgagtcc gttgcggccg ttgaaaaacc tcaagggacc gaactcgttg ccaccggtat
     1321 tgaaagctga ggtgagcctg tcaagaaaaa catctgccgc catctcggat tcaagctgga
     1381 aggaaccggt tgtctgtccg aatgagtacc agataagccc atgtgtcagg tcaaactcac
     1441 ctcccgcact ttgcctgttg gaggagaaga ccaccggaag cctggcatca atccttcccg
     1501 cttcaatatc catattgtag tcgtcatgct ctgtattgag cccctcaagt gctatgaccg
     1561 aatcagggaa aatgcccagc tcatagttga ttttcaatgg ttcctcgcga caacctgtga
     1621 agaaagggac aattgacaaa actgcaagaa aaaatagtaa cctgctgctg gtcatgacag
     1681 tcgtttattt tataacgggc agcaaagatg tttaatatga gccaaatgaa aaaaaccgga
     1741 cccggggccc ggtttcagaa ggtagaattc aggttaattc tagggtaata tttggtttga
     1801 gggtaagctt ctgaactcaa ttgtaagatg gattccggca tgggaaggtt gcgtcagatg
     1861 aatttttttt tacaaatacc tgtttatcat gtttataaaa acgggtgtct gttgataatt
     1921 gctgcccggt ccgggctgac tgaaccaata atatgttaaa tttgcagaat atttttcggg
     1981 gagatggaag aagaaagatc cggttttgtg cacgacagtg agttggatga ggtgattctc
     2041 cggtttgaga gaatgcggaa gaacaaggaa acctgctatt tcgacgtcaa tgagtttgag
     2101 accattatag actattacct tgagggcaac aacccggttc acgcctttga ggctacctca
     2161 cttgcatgtg aactgcatcc caactcagtt cccatcctta tcaggaaagc ccgcgtactg
     2221 cttgaaaagg gaagggccac cgaggctctt acaatagcca ggcggcttga aaacattgag
     2281 cccggcaact gtgatatcta cctttgcaag gcgacagcgc tggcaatgct tggtgacatc
     2341 aacggggcac gcaaggtatt cgaccttgcc ctggaaaccg attcggatga tctcgaaaat
     2401 atcctttaca gcataacatc agtgctgcag aacctcaatt ttcacgagga aatgattccc
     2461 tacctcaaca aactggccga acttgagccg gatttccctg ctcatctgta tgaccttgca
     2521 tatgcctatg acaagaaggg tgaccagcgg acaagcataa aatactataa cctctacctg
     2581 gacgaggatc cgtactcaga cagcgcatgg tataatctcg gtataatata caataaaaca
     2641 ggtgattacg gcaagtcgct cgaggcgtat gattatgccc ttgccctgaa tcccgaaaac
     2701 accttcgccc tcttcaacaa ggctaacatc ctcagcaacc tcgggaaata cagcgaggcg
     2761 atggatgtct accgccagtt cgtggaggag gagcctgaca gcctcgaggg gctgacttac
     2821 cttgccgagt gctatgacaa gacaggcgat gaggcgatgg cacgcaagta ctacgccgag
     2881 gccattgata tcacacctga ttttcctgat ccgtggtacg gcctcggact gctgtcgctc
     2941 tcgcgcaaca ggcctgctga aagtatcaca cccctgaaga aagccattga acttgacccc
     3001 gagaatcccg actactggtt ctcacttggc aaggcccata tcatgaacgg aaacatcagg
     3061 gaggcagtaa gatgctatat cgaagctctg atcattgatg gctactatga cgaggcatgg
     3121 atcgagacgg gcatagtggt gatgatgacc ggagcctaca ggggagcaat ccgcattttg
     3181 aaaaaagccg cgaaaatcag cggagacctg ccaggaatat acttcctgct ggcatcggcg
     3241 tatcttcaca cgggcaatat gaaggaagcc acccgcaacc ttgtcagggc tataaggctt
     3301 gataatgaac tccttgaaga gtactccgtg ctcctgccgg aggaaaagct gaccaatgca
     3361 atgagacgac tgttcaggaa aaccaactaa gatatgaaac aagcgagaga actgctgaca
     3421 cacattccgg taaggccggg taagccgaga aaagaggggc ttacaatggt aatggataag
     3481 ggactgagtc tcagggaggc tgaagacttc gccggggtgg gcagcgagta tgttgacttt
     3541 gtgaagcttg gattcggaac atcggtaata accccgaatg tcgacaaaaa gataaaaacc
     3601 taccatgagg caggaatgac cgtctacttc gggggaaccc tcttcgaggc attcattatc
     3661 aggggacttt ttgccgaata cgttgacctg ctgaagcatg caggcgtgag aatggttgag
     3721 atctccgatg gctcatatga gttcgatcat agtgaaaaac ttcagtatat caggaaactg
     3781 tcaaaggatt tcacagtgat ctccgaggtc ggctcgaaaa agaagaatat tatttatacc
     3841 cctgcccagt gggtggagat gatgaaggcc gaacttgatg ccggctcggt gaaggttatc
     3901 gctgaggcgc gcgaatcggg cacaattggc atctacaatg atgacggctc ggtgaataag
     3961 gaccttatcg gcaccatctc acgggagata ggatttgaca acgtactgtg ggaagcaccg
     4021 ctgaaacagc agcaggcgtg gttcattgcc cacttcggcg ccaatgtcaa cctcgggaac
     4081 atagccccga atgagataat tgcacttgaa tcactcaggc ttggattgcg aagtgacacc
     4141 tttttcagct tccttcctga ccacatgaaa tgaggctcta cggaataata ggatttccgc
     4201 tcggacattc attctctcag aagcatttct cggaaaaatt tgaaagggaa cacctttctg
     4261 atcatgctta ccgcaagttt gagctgccgg aaataggtat gcttcctgaa ctgcttgtgc
     4321 gggaacctga cctctgcgga ttcaatgtga ccatcccctg gaaggtgaag gtgatggact
     4381 tcctggatga tatagatccg gtagcagctg aaatcggtgc agtcaatacc gtaaaggtca
     4441 cccgccacgg cgggaaagcc tggcttaagg gcttcaacac agacgcacct gccttcaggg
     4501 aatcacttct cagtaacctg gataccctgc ctgacagcgc cctggtcctc ggtaccggcg
     4561 gagctgcgaa gagcgtgatc catacactta ttgatctgaa tatcattgct gtaccggtgt
     4621 cgcgcaagcc agtccgcggc ggatatacct atgatgacct gccgggatac atgatcagcg
     4681 aggcaggggt aataatcaat accacaccgg tgggcatggc acctcacact gaagctatgc
     4741 ctcccattga ctacagctgc ctgcgggagg gacagttgtt gttcgacctc atatacaatc
     4801 ctccggttac ggcattcctg tcaagaggag aggagcatgg atgcatcacc gtcaatggtg
     4861 aggagatgtt ccttattcag gccgggctgg cctgggagat atggaatgat gtggagtgac
     4921 aaagcgcagg cgaatacctg ttattccttg tgctgccggt aatctgccgg tcggctgctg
     4981 caggcacctg aaaatcaaat gaatatattg ccgggatatc caattgccgt cagtgaaagc
     5041 ccgtgtgcag gtgcagactg tcctgcaagt ccccggtttt tcccgcggag aatcgcgtcg
     5101 aagtcgttca gagccagctt ccctctcccg gccgggataa gtgtgccaac cacagccctc
     5161 accatgttcc tcaggaacct gtcagctgta atggtgaaga tgagcttttc ctgctcgtca
     5221 gtccatcgcg catgcgtcac ccggcaaatg ttggtcctgt tgctgccgtg aagccggcag
     5281 aagctggtaa aatcgctgtg cttcagcagt atcgctgcag cctcattcat cctgtcaatg
     5341 tcaagctgcc agtagagcag ccatgcggtg tcggcacaga acggatcctt caccctggtc
     5401 acggtatact gatatgtccg ggaaatggca tcgaaccttg cattggcatc gggatttacc
     5461 ttcacgatac gttttatcgc aatgtcatcg ggaagtatgc cgttgagatt gtaaagcaac
     5521 tgcccgtcat tgttgaggtc gttacgcacg gcatcaaaat gggcacagaa aaaagaggca
     5581 tgaactccgg tgtcagtccg accggctcct gtgaccgcta tctgcgatga gagaactgtg
     5641 ctgagcgcct tagtgagaac accctgtacg gtgtgcttgc cgggctgaat ctgccagccg
     5701 ttgtatgccg tgcccctgaa ggaaagaaaa atgaagtatc gtgtcatgtc cggaccaaaa
     5761 tgtaaagata gctaaagaac attggcaggt agattgcttt taagtaaatt gcggcgacca
     5821 aaaaacaata cattcatgag cgatccggaa ctcaagagag gctttgccag cgataacaac
     5881 tcaggcgtcc atcccgccgt gatgaaggaa ctgttgcggg tcaacagggg acatgtggta
     5941 ggttacggcg acgatccgtt caccgaaacg gccatgacgc tgataaagaa ggaactggga
     6001 gctgaaacgg aggtatattt cgtctttaca ggcaccggtg ccaatgtgct gggactcgca
     6061 ggggtgatga aatcgtggaa ctcggtcatc actgcgcata cagcccacat cgagactgac
     6121 gagtgcggcg ctcctgaaaa atttaccgga tgcaaggtgc tttctgttga cacacctgac
     6181 gggaagctgc gcccggagat gctcgccagg catatgcacg gatttgattt tgagcaccat
     6241 agccagcccg gagtgatctc catctcacag gccacggaaa tgggcactgt ttatactccc
     6301 ggggaggtga aggagctggc agactacgcg catgaacacg gtctgctgct tcatatggac
     6361 ggcgcccgca ttgccaatgc cgcagtcacc ctgagcgttc cgttcagggc attcaccacc
     6421 gacgccggcg ttgatatcct ctccttcgga ggaaccaaga acgggatgat gtacggcgaa
     6481 gctgtctgtt tccttaaacc ggggctctcg gagggattca aatacctcag gaagcagggg
     6541 atgcagctgg cttcaaagat gcgtttcatc tcagcacagt atatcgccta tctttcaggc
     6601 ggattatgga aggagtgcgc ctcacactcg aacaggatgg ctcgggaatt gcaaagaagg
     6661 ctgaaggaga tcagcggaat aaagatcacc cagaaagtgg aggccaatgg cgtcttcgtc
     6721 atcatgcccg acagggtggc cgcactgctg agcagagagt actttttcta cccgtggaat
     6781 gaacagacat cagaataccg gctgatgacg agctgggata caacttcaga tgacgttgtg
     6841 caatttgcag acaggctgaa aaatatgatg tcagattata agtagtctcc tgaccctttc
     6901 tgtatctcgg ctacagagaa aggaagatca aaaacagcgt ggtgatgatt caggttcgcg
     6961 gagccagcag catgctgtcg ccataactca ggaaacggta ccctgaagcc agggcatgat
     7021 tgtatatctt tttccagttt tccccggcaa aggcagctac cagtagcaaa agagtactgt
     7081 ggggctgatg gaagtttgta accatgctgc tgaccatcct gaacctgtaa cctggaacaa
     7141 tgatgatgtc agtgcgggct tccagcgtgt ccctgccgct cctttcaagt tcagccagga
     7201 ggcagtcaat tgcgacggca ggatcaagtt caccctcttt ttcgtaaggt tcccactgtg
     7261 aaacggatgg gattcggtct ttgaactccc tgccttcata aaggctcatt ccgagccagt
     7321 agaggctctc gagcgtgcgg acggtggtgg tgcccactgc ggtggttttc ctgcttctga
     7381 ggcgctccaa agtctgtttt gagacgaaaa agtgctcgct gtgcatcatg tggtcacaga
     7441 tcagctctga tttcaccggc ctgaatgttc cggcgctgac atgaagagtc acctccgccc
     7501 gtggaatacc tgccgcatca agtccggcga gtacagaagg cgtaaaatgg agtccggcag
     7561 tcggggccgc cactgaaccg tttattcgtg catatgtggt ctggtatgca gaaacgtcgc
     7621 tctcctcatc atccctgtcc aggtatggtg gtatgggcat atggcccagg cactcgagca
     7681 ctccggcaaa ggaaagaccg gcctcattcc agctgaacct tacagtgaag gcgtctccgg
     7741 ctgtccctgt acgttctgca ctgaggatat gctccttgcc atcatgaatg aaccgtgatt
     7801 ccagcattcc gcttttccag cgcttgaggt tgcctacaag gcatttccat tccaccgggc
     7861 cacccgatgc caggttcatt gagaattcag ccggactgtg cggttcaaga cagaagactt
     7921 ctataatggc gccgctggct ttcctgaaga taagccgcgc acggataacc ctggtattat
     7981 tgaataccat caggctggca tggtcggtca gggccggcaa ctcactgaaa atatgatcac
     8041 tgatgttacc gtcccacacc agcagctttg acttgtccct ttcgggaaga gggtatttcg
     8101 ctatcctctc cgggggcaga tcataggtgt aatccgctat ctttatgtcg ccgtagctca
     8161 tcttcatttt ttcgtaacgg gcaaaaatag ctaattttga gatcagatga caggtcatga
     8221 aaggaagccg cagcagccgt ccgccctcag gcggaagtaa ggtcagggaa attgaccttc
     8281 acctcaggcc gggccaggca ggggttgaag cgctggaacg tcagctggca cgggtcaggg
     8341 gagagcttaa cagctcaatc aaggcaggag cgagagagat cgttttcatt cacggttcgg
     8401 ggagcggtaa gcttagagag gctttgcgtg ccgccatcgc agctgaatat ccttcctgca
     8461 cctgccatga tgcaccgttt gccaggtacg gttataatgg agctacaata gtgacaatca
     8521 gaaagtaaca gaaatggcat aaatcagttt cgatccgtgg tggcagaagc aggtcgctgt
     8581 ttttgaggag atggatgatg atctgatcgg tgagatcacc gcgagggcat ggaactcatt
     8641 cacagattgg cacgggcttc cgcccgaaga gatgtgcagg ccactggaag caattgctga
     8701 tctcctgatt cggaataatt gactatcttt gccaccggca ggccggtccg tcgctcctgc
     8761 actcaggtgc cggggaggaa agtccgggca acgcagagca ccatccttcc taacgggaag
     8821 atatccgaaa gggtatagca gtgtaacaga aaagaacagt cccgctccgc gggtcaatgg
     8881 tgaaaaggtg aggtaagagc tcaccggttc tggtggtgac accgggagcc gtacacctga
     8941 tgggttgaaa gaccatgtat accggcgtct gagggctgct cgcccgagcc ggggggtagg
     9001 tcgtatgacc gcgccagcaa tgacggggca agataaatga cggacaggga acaaaatcct
     9061 ttactggaac cgtctcccga cagaacccgg cttacaggtc tgcctttgat acaggagatg
     9121 ccgcccggcg tgggcggcat cttttgtgtg tgcccggcat tggcgataac tatgcggagc
     9181 gcacaccagg cacttagtaa atgccgggat tacatcacgg aaggctatgt atatgcagtg
     9241 gacattgatc ttgagaggtt ctttgacacg gtgaaccatg gcaaactgat agaggtactg
     9301 tcacgcaagg ttaaggatgg gcgggttgtc tcgcttatcc acaagtatct caatgcagga
     9361 gtgatgaagg aaggcagata cgaagagacg ccggcgggtg ttccgcaggg aggtccgtta
     9421 agtcctgtac tgagcaacat attgctcaat gaactggaca ttgagcttga gagacgggga
     9481 cacaagtttg tacgctatgc agacgacatg gtcatattat gcaggagccg acggagtacc
     9541 gaacgtacaa tgcagaacat catctccttt atagagggga agttgctgct gaaagtcaat
     9601 cgggacaaga gtaaggcagc ctatgtcagg gaagttaagt tccttggcta atccttttac
     9661 atctacaagg gggagggcag actgcgtata catcccaaaa gcgtatctaa gatgaaaact
     9721 cgtatcaagg agctgacatc aaggagcaac ggatggggca atgaccggag gaaggaagca
     9781 ttgagccaat acattaaagg ctggttgcaa tacttcaggc tggcagacat gaagactctt
     9841 ctggaaggga tagatgagtg gtatcgccgg aggttacgca tggtcatctg gaaacaatgg
     9901 aaacggataa ggaacagaag tagtaatctt aaaaggttgg ggattagtaa atcccaagcc
     9961 tatatgtggg cgaacacaag gaagggatac tggcgcattg ccgggagtta tatcctctcc
    10021 accaccatca cttccgtgcg tcttcgtcta tccggctacg ttttcttcac agactattac
    10081 agtaaggtta gagtggtctc ttaaggaacc gccgtatacg agacccgtac gtacggtggt
    10141 gtgagaggtc ggaaaataaa gtaggaggaa aactacttta ttttcctcct acatgatttt
    10201 atccaggata ttttccggtc cgggtggcga caggtctgag accttttgca ccggcaccca
    10261 tttgcatatt acaatttgaa gattgaatcc tccatcgggg tgtcaaactc aatcgtttgg
    10321 aaggtcatgc ctcccagctc ggatccttca acgaactggg cgatcttggt cccgaatttg
    10381 acgccattga tctctttccg ttccctgata taggactcaa ccagcatttc ctgccccatc
    10441 tggctgacgg tctgggccgt cttgacaggc aggaagctct ctttgtcaag gtatgtggtc
    10501 gaagtggcac cagattcact tgtgacggtg atcttatagg ccggtttgcc ttccacggct
    10561 tcctcaccct caagcttcat tctcccggct ttgaaagcct ccatgaaaga gtctttgaac
    10621 acgttgtact cctgcacctg gcccaattgc tcggcaggaa gttcaaccgc ttcagtggcg
    10681 ccggtgagcg gattcagcat aaatcccttt tcaccgtcga aggctatgac gatctccatt
    10741 ccgttgaact cctgaattac cttgacctta tttggttttt tgaccataat ggttattggc
    10801 aattccatgc ccatctggct gacaacgcct ttcatagtca tggtctgagc cttctccagg
    10861 ttctcaatcc cgttggctgc atagtacttg tttgcaatct cgtcgagcga ctgggcactg
    10921 cagactgatg ccaccagaaa tgccgccaga agagtcattg attttttcat aataatttaa
    10981 tttgttgttt attaaaagga gttaactgta gttcttatta atgtaagttt ataaagcagg
    11041 ctgtcgagca tatcgagcct cagcatgttg gctccgtcag agagggcaga ggccggttca
    11101 gggtgagttt cgatgaagat tccgtcaacg ccggcagcaa tggcgcacct gccgatggtt
    11161 tcaatcatgt caggctgtcc gccggataca cctccttcct ggtttggctg ctgcagcgaa
    11221 tgggtgatgt ccattatcac cggagcgccg atctgcttca tcacgggtat gttccgcata
    11281 tccacaacca ggtcattgta cccgaaaaag cttcccctct cggtaagcat cactctctca
    11341 tttcccgatt cccttatctt tcctgccgca aagcgcattg aggcaccgga aagaaactgt
    11401 cccttcttga catttaccac ctttccggtg gcagctgcag caacaagaag atctgtctgc
    11461 ctgcagagaa acgcaggaat ctggatgaca tcgacgaaat gagcggcaat gcgggcttcc
    11521 tcaggcgagt gaatgtcagt aaccaccggc accgaatact tctcccttac ctccgcaagt
    11581 atctcgagcg ctttctcgtc gccgatgcct gaaaacgaat caagccttga ccggtttgct
    11641 ttacggtatg atgatttgaa aatcaacgga atatggtact tgtcgctgag cttcaccacc
    11701 tcggcagcca cgcgcagcgt catctcctcc gactcaacca cacatggtcc tgcaagaaga
    11761 agaaaattcc cgctgcctgt atgctttagc aacggaatat tcagttcact gactttcatg
    11821 agataaaatt actaaatatt ctattcgtta tcctttttgt atctatagat tttttgccac
    11881 cgggtctcca gttgctttcg tatctcaagc tcacgggcat tcctgcccgg atcgtaaaag
    11941 accgtaccgg ttatctgctc cggcaggaaa ttgtcctgga caaaattgtc ctcgaaacta
    12001 tgagcatacc tgtatccttc atgatatccc aggttcttca tgagcctggt gggcgcattg
    12061 cgcaggtgaa gcggtaccgg caggtcacca tgctgcctga cagcagctat tgcattctca
    12121 attgccatgt aggaggcgtt gctcttgggc gatgtggcaa ggtaaactgc tgcttcagag
    12181 aggattatcc ttgactcggg ccagccgaca agatttacag cgttgaaaca tgcctgtgcg
    12241 aggagcaggg cattgggatt ggcaagaccg atgtcctcag ctgcaagtat taccattcgc
    12301 cgggctatga acttcacatc ctcaccacct tccaccatcc ttgcaagcca gtataccgcg
    12361 gcattggggt ctgacccgcg gagcgacttg ataaatgctg aaatgatgtc ataatgctgt
    12421 tctccgttct tgtcatacat agcaaggttc tgctgcacat ggccaagcac catctcatcg
    12481 gtgacaacaa tctccccgct gcccttctcg gcctcaaggg agacagtcag ctcgagcgca
    12541 ttgtacagct tacgtgcatc gcctccgctg atgcgaatca gtgcttcata ctcagctacc
    12601 ctgattttgt accgggagag gtaataatca tctgtgactg ccctgtcaat cagcttcctg
    12661 agggtttcgt catcaagagg tttaagcaca tacacctggc accttgaaag gagaggggat
    12721 attacctcga aggatggatt ctctgttgtt gcacctataa gcgtcagaat tccctgttcc
    12781 acggcaccca ggagtgagtc ctgctgcgac ttgttgaaac ggtgaatctc gtcgatgaaa
    12841 agtatgggat tcggcttgcc gaaaaactgc tggctctttg ccttttcaat cgtctcacgg
    12901 acctccttca ctccgctgtg cactgcgctc agctggaaga aggaacgctc cagcttgttg
    12961 gcaacaatcc ttgcaagtgt tgtcttcccg acaccgggag gaccccataa tatgaatgac
    13021 ggcacattgc cggactcaat caccttcctc agcaccccct cactgcccac aagatgctcc
    13081 tgcccggcaa actcctcgag tgaccgcggt cttaacctgt ccgcaagtgg ctgatttgaa
    13141 aacatatggc aatttcacaa ttacaggtgc aaattatcac aaaatcgctt gaaccgttac
    13201 gttttttgcc attttccgat aaaatatttt cccgatagga aataatttgc tactttagcc
    13261 attatttttt tacaacacgc atactgtacc ttccaagaat tcgtttagta acaggatatt
    13321 atcaaattcc ggtaattatg aaaaaacctc tactgataac attgttagtt ctggcagtac
    13381 tgctcttaat accggctgtg agctttatca gatgggcttt ccaggaaaag aaacctgtcg
    13441 atgttgtcat acttgacaag acagtaccga cacttgacag actgggtcac cgctcatttg
    13501 tctacatcct gactaacgct agatatgtaa ggggagaaaa gggtggcagc tattcagcaa
    13561 acaaggacta ttacggcttc gaaccactca gacccgtgag ggagaaacag ttcaggaaaa
    13621 gggacttccg cctgaccgaa ctgattgacc tggcagataa gaatgacgta ttgtattaca
    13681 ctgacactta cggtgtgttc ttcaacgact ggtaccaggg gataaagaag acacgcaggt
    13741 caaggaagct ttatggcggc ctcaacaaca acgattatct ccttatggtg gaaatgaaaa
    13801 aacgggataa gctggttctc gttgaataca atacattcga ttatcctacg gcgggacttg
    13861 agagattcaa gacagaagag cttctcggaa ttgcttcgaa aggctggatg ggacagtact
    13921 acaagtcgct cgatactgta tcacagcagg gcgtgcctgc ctggatgccg gctctctacc
    13981 gtaaacagta ccgcgaacca tggaccttca ccaaaccggg agttgtactg ctcagggaga
    14041 atggcatcat agtgctggag gagggtaaac atcttgcctc agcattgccg gtcatcagaa
    14101 caacaggacc atcagcagag aggttcgggc ttcccgagac ggttgccttt accaatagct
    14161 tcgatatcat agacccggga aggaacaacg tagtttccga attcgtcctg aatacaactc
    14221 cttcgggtga tacactgctg gcagtcaacg atctgcaggc tgtattcccc gcagtcacac
    14281 aggaacctct cactcagaaa acctattact tctcaggcga tttcgcaaac aataatatac
    14341 ccttctggac agccaggatt aagaacatcc agaaaggagc gaagagtttt ctttattctg
    14401 aaaagccgga tgatccgaaa agattcttct ggctgtacta caaaccgctc attacatcca
    14461 tcttcgcgga ataccaggca gaacatgccg gaaagtagta caatgacaaa cagcagaaaa
    14521 acaggtccac gggcctgttt ttttatttat ggcggtatcg tcgtgtttgc tgctgaacac
    14581 atcattaccg tcgctgtttt ataaaggtta cttcatatct ttatgtgcag atttgcagca
    14641 atgaaagcaa caatagtaca gtgcgggatt aagtgggagg acagggactc cagtctcgcg
    14701 catatctcag ccctgcttga tgcagctcct cccggagggg ggattgtggt gctgcccgag
    14761 atgttctcca ctggctttac catgaatccg gcaccccttg cagaagggat ggacgggcca
    14821 accgtaaggt ggatgaagga acgggccgcc aacggagggt atgccctctg cggatcggtt
    14881 atcataggag aggaggacag gtattataac cgcctgatat ttgtgactcc agttggggac
    14941 gtgacattct acgacaagcg gcacctccat acaatgagcg gggaacatac tgtatacagt
    15001 cgtggcaata agcgggttgt ggtgacttac cgcgagttca gcttcaatct tcaggtttgc
    15061 tatgatctgc gctttccggt atggtcaagg aaccgcggcg acaccgatgt aataatatat
    15121 tcggccaact ggccttcggt ccgcagctct gtctggaagg cgctgctggt agccagggca
    15181 atagagaacc agtgttacgt cattggagtg aatcgcgtgg gcgccaatcc cgacggcact
    15241 tcatataccg gcgattcggc cattgtaagc ccgaaaggtg agcttatggc ttctctcggg
    15301 ccaggcgccg agggcgttgt ttcggcgatg ctctcacgtg aggtgcttga caagtaccgt
    15361 gaggatatgc ccatctggcg ggatgcggat gcatttacga ttttgtgatt tacgatttgc
    15421 gatttgctat tttgggcagg ccatcaggaa ttgtaaaagc aggacatgaa atcttcagcc
    15481 tctgagagca gcgaggagca cttctgcagg atgaagtgct accctgcccg taccgtcctt
    15541 gacatgatgc cggcagctgg tgccgggggc gcagatgatg gttccggcag atgcagaccg
    15601 tacatctggg aaaagaacca gttcaccaac cttctgcgcg agctcaaaat gctctttttc
    15661 atacccgaac gcccccgcca taccgcaaca gccagacggt atctccctta ccttatagtt
    15721 taccggaata ccgagggcag ccagcaccgg tgccgaagtg gtgatcgctt tttcctggca
    15781 gtgaacatgt accagcacct ctgcaggctc acgggtgaac atttctctct ttattcttcc
    15841 tgcactgaac tccccggcaa tgaactcctc aagggtaaat gtgtgctgag ccagccttga
    15901 agcctcgggg cgcaacttat caccggcaag atcaggaaac tcatccctga agccgaggat
    15961 cgccgagggc tcgatcccta caaggggaca gtcatcgctg accagcggcc caagtaccag
    16021 tatattttta gttatcagct tctttgcctg acgcaaaaag ccttttgaaa tatatgtccg
    16081 tgcactggca gcattcccgg caaatgtgat gcggtagccc agcccggtca gcagtttcgc
    16141 tgctgccttg cctgcatcca ggtcgttgtg gttggtgaac tcatccacga acaggcatat
    16201 ttcaccgatg ggattagccg gatttagtgc agacagattc tttttcagcc acctcctgaa
    16261 ggtctgcggc gccagcaccg ggatgcttcg ctctgcggca aatcctgtca ctctcttgat
    16321 tatcagtgcg gtgaatttgt tggtagcaaa gaaattaaag atacccggaa taaatgagaa
    16381 aaggctgtag atctttggca gcgaggcaat cagccttgtg cgcagtgacg gcggatgcac
    16441 atcattatag tgctgcagga actccgactt caatttggct atatcaaccc ctgaagggca
    16501 ctcgctgcga catcccttgc atgccaggca caggtcgagg atctcgtata tctcgcggct
    16561 gtcccaggga ttgtctctgt cggcatagag catttccctc attatgtttg ccctggcacg
    16621 ggttgtaagc cgctcgtcac cggtggccat aaagctgggg cacatcagac cgcctataac
    16681 actgctcttg cggcagtcgc ccgatccgtt gcacttttcg gcggcacgca cgacacctcc
    16741 gcagtcggag aaatcgtaat gcgtcgccgg ttcaggggta ggcttccccg gaatatagcg
    16801 cagcgagctg tccatgggag gggtgtcggt aatcttcccc gggttgagta tgccgttcgg
    16861 gtcaaaaacc ctttttatcc tcttgttaag cttgtagttt ccggcgccga taataagcgg
    16921 tatgaactca ccgcgcagcc ttccgtcacc gtgctctccg ctcattgagc cccggtattt
    16981 tttcactatc cgggcagtct cttcggcaat ggtgcgcaaa agcttcgcct caccggcatc
    17041 cttgaggttt atcagaggcc tgacatgaag ctccccggtg cctatatgtg catgatatac
    17101 agactctttg ccgtaagagg ccagcatctc ctctatgtcg gccacgaagt cgcccagcct
    17161 gttcacatcc acagcgcagt cctcaatgag agagatcggc ttggcgtcgc cgggcatgtt
    17221 tgacagtatt ccgagccctg ccttgcgaag gtcccagacg cgcttaacgt cggcacccca
    17281 cacgaccgga aatgcgtatc ccagtccctc gctgcgcatc tcctcctcca tgactgccgc
    17341 agtgaagatt atctcctctc ggctcccggc gcaaaactcc actatgagca aagccccggg
    17401 gtcaccctcg atgaagaacc tgttccgttt ctggctctct acggtggctg ccaggctgag
    17461 tatgttgctg tccatcagtt ccacagccca tggctggtgt ctgagtgcaa tcaggttggc
    17521 gcggaaggcc tcgttacggt gatgcaggtg aacgcatgcc agggccttaa ccggcggagg
    17581 cagcggcacc agccccattt tcgcctcggt gacgatggca agagtccctt ccgacccgca
    17641 gatgagcctt gcaagattga tcttccgacc cgctccgggt gtgaaaggtt cactgcagag
    17701 caggtcatca agtgaatatc cggtgttgcg gcggggaatg gtcttctcag ggtagccggc
    17761 catgattgat tcccggtgac cgggatctgt cataatctgc tcgatttccc tgtatatctc
    17821 accctcagcg gtttgcaggg agcacttctg actgaactgg tccgcggtga gctcaccgaa
    17881 gacgacttct gatccgtcgt gcagcaaggc actgaccccg attatatgat cccttacgga
    17941 accatagacg acggagtgga gtccgcagcc gttgttaccg atcatccccc ccaggttgca
    18001 ccggctggaa gtcgacgtct ccggcccgaa cagaagtccg tggcttcgca gggcggcatt
    18061 gagctcatca ggaaccaccc caggctcaag cctgacccat cgttcttcga cgtttatttc
    18121 cagtatttta ccaaagtgac gcgagatatc ggccactata cccgaaccta ccacctgtcc
    18181 tgccagcgag gttcccgcag ccctcgggat tatgccgatg cggtgtttcg cggcaaacgc
    18241 aagaagcctt tttatgtcat cagtattgcg cggaaagact atcgccagcg gctcctccct
    18301 gtagatggag gcatcggtgg catacatgag cctggaactt ctgtcggtgc gtacctcacc
    18361 ctcaaagcca gaggtcagca gttctgtcaa tgtggctgtg cgttgatcct ccatctgagc
    18421 aaattcagca tcaaaaatag ggatttgaag ggttcgcggc agggccgcaa agttgttttc
    18481 gaaaaaaaat gactaaaatt tgaaaatact gactataatc atgttgttga ttattgcggg
    18541 attacacttt tttagtactt ttaacccgac acaatactca aactaaatta ctgatatcca
    18601 atggaattgc ttgaacagat caaaacgaat gccagaaggc ataacaaaat gattgtattg
    18661 cctgagggaa cggaggagag gactcttaaa gcagcagatg cggtgctggc agaggggatt
    18721 gcacgtataa ctttactggg gaatcccgcg gagattaaga cgatggcaga atcgtttggt
    18781 ctgaagaata taggaaaggc gcagatagtt gatccgcatg atcaccccgc gaagaagcat
    18841 tacattgaca cgatggttga gctccgcaag agcaaggggc taacggttga ggaggcttcc
    18901 aggctgattg aggatccgct ttatctcgga gtgatgatga tcaagtgcgg tgatgctgac
    18961 ggagaagtgg cgggagcaag gaacacgaca ggcgatgtgc tgagacctgc cttccagatt
    19021 gtaaagaccg ccccgggcat atcagtggtt tcgggtgcat tcatcatgat gctgaaagac
    19081 aaggagtttg gtgcggaagg tataatcgtc ttcgccgacg gcgcagttca ccctgatcct
    19141 acggacaagg aacttgccga gatagcagtc gccactgccc gcaccaccaa aactattgtc
    19201 ggtgtggaac ccaaggtcgc cctcctcagc ttctccacca tgggcagcgc aaagcacccg
    19261 ctggttgaca aggtagtcag tgccacaaag atggcgaagg agatggctcc ggaattcatg
    19321 tttgagggtg agcttcaggc agatgcagcg cttatcgaag ccatcggaca gaagaaggct
    19381 ccgggcagca ggatagcagg caaggccaac gtcctcgttt tccccaacct ggaatgcgga
    19441 aacatcgcgt acaagctggt tgagagactg gcacatgcac aggctatcgg ccctgtcctt
    19501 cagggcatgg cagcgcctat caacgacctc tcaaggggtt gttcagtcag tgacatcatc
    19561 aacgtaatcg ccataaccgc caaccaggca gccggcttaa ccaggtaaca caaaggaaat
    19621 catgaatatt ctcgtactaa actgcggcag ctcttcaatc aagtatcagc tttttgacat
    19681 gcaggacagc gccagggtgc tggcaaaagg actgattgaa agaatcggca tcacagacgg
    19741 catcctcact cacaagccaa ccggcaagga tccctataag gtggtcttgg acataccaga
    19801 ccataccgtc ggtatcaaca tggttatgga tgcactggta gacccggtgc atggcgtcat
    19861 aaaaagcatc tcggagatta aggcagtagg tcaccgtgta gcgcacggag gcgaaaactt
    19921 caaagagtcg gtgttgattg acaacgatgt caagcgcgac atagaaaagt gcgccgagct
    19981 ggcacccctg cacaatcccg ctcacctcaa ggggatcctc tcctgcgaga agctgctgcc
    20041 gggtatacca caggttgcgg tcttcgatac ctcgttccac cagacgatgc ctgattatgc
    20101 gttcatgtac gccatccctt atgagtatta tgaaaaatac cgtatccgga agtacgggtt
    20161 ccacggaacc agccacaggt tcgtggctga gaaggcctgc aaaatgctga acatggacct
    20221 ttacaactca aagattataa cctgccacct tggcaacgga gcctcaatca ccgccattga
    20281 aaatggtaag tcgattgaca catcgatggg cttcaccccg gttgacggac tgatcatggg
    20341 tacccgcacc ggtgagattg acccgggcgt gttgctctat ctggctgaca aggaagagct
    20401 tagcgtgagt ggtgtaaata acatgataaa taagaagagc ggtgtggcag gcatttcaca
    20461 gctctcatcc gacatgcgcg accttgaggc ggctgcaaat gagggtaacc cgaaagcact
    20521 cctggcgctc aatatgtatg cttacaagat caagaagttc attggcgcat atgttgcagc
    20581 aatgaacgga ctcgacctgc tcatcttcac tgccggagtg ggagagaacg acttcaatgc
    20641 aaggaagatg atctgtaccg ggatggagta tatgggcatt gaggttgacg aggatgtgaa
    20701 ccacggcgtg aagggaaaag atctggttat ctcgaagcct tcctcgaaag tgaaggtgat
    20761 ggtgattacc acagacgagg aactggtgat tgcatctgac acccaggcca tagttgagaa
    20821 actgactcac taaacactct gtataatggc tttcaagcac ctctccgaac tcaagcaact
    20881 gcttgtagac aagcctgcaa ggcgcctggt actggctgcc gcgcaggacc acaattcact
    20941 ctcctcggtt ctcatggctg cacatgatgg cttcattgaa cccatacttg tcggtgacaa
    21001 ggatgcgata cataatatcg cagctcagaa caattttgac ctgactggcg taagggtggt
    21061 gcacgagcct gattctgaaa tggctattga gatagctgtc aggatggtcc gcagcaacca
    21121 ggcagacatc ctcatgaaag gaaaggtggg tacttcaaac ctgctcaagg gagtcctgaa
    21181 caaggagtgg ggactgagat cgggctcgct gctttctcat tttgccattt ttgaggtggc
    21241 tgcttaccac aagctcatcg ctgtgacaga cgtggcgatg aatattgcac ccaacctgca
    21301 ggacaagata gcaatagtga acaactccgt cgcctgcctc aacaggcttg gtgttgaaat
    21361 gccgaaggtt gccgtactgg gcgctgtgga gatggtaagt gagaatatgc aggccaccct
    21421 cgatgcagct ctcctctcga aaatgaacca gcgtgaccag atcaggcatt gcctcattga
    21481 cgggccgctt gcctttgaca acgcaatcag ccttgagagc gcaacgcaca agggtatcag
    21541 aagtgacgtg gcaggcgata cagacctgct gctgatgcca gatatcgagg ttgggaatgt
    21601 tctctacaag tcactggtat tctttgccca cgccggcgtg gcggcagtga tcctgggagc
    21661 aactgcacca atagtgctga cttcgagaag cgactctgac caggcgaagt attacagtat
    21721 tgtgcttgcg gcagcaataa caaggaagga ataaaatgat tacaagtctc aggcagatgg
    21781 ctgacaccgt caggtcgtca ggaactaaaa agaaaatagc cgtcgcctgg gctcaggacc
    21841 cgaacaccgt cggatcactt cacagggctg ttgcagaagg atttgcagaa gccataatga
    21901 ttggccggcg tgaccggatt cttgccactt gccaggagac aggagctgat ccccgattat
    21961 ttacactggt tgaatcacct gatgaaaaaa gtgctgcgga actggcagtg tcaataacca
    22021 ggggtggtga ggcagacgtt gtaatgaaag gccttgtggg cacagacaag ttcctcaggg
    22081 cggtgatgga cagggagaag ggtcttttgc caccaaaagc tgtaatgagt tacgtctgtg
    22141 cagttgagat tccggcatac cataagttgc tgtttatcac tgacactgcg gtaatcccgt
    22201 ttcctgacct cgatcagaaa gtggcaatgg cccgttatgc cgtggatatg gcacgccgtt
    22261 tcgggatcgg gaaaccgcgg attgcactga ttggtgcatc ggagaaggtg agcgaccatt
    22321 tcccgaatac cctcgattac tcagttatgt gcaagatggc agagagagga cagataccgg
    22381 actgcatcat ggatggcccg gttgacctct tccttgcatg tgatcctgag agtgtcagga
    22441 tcaagggggt ggacacaccc ctggccggcg atgctgacat tctgctcttt ccgtcactgg
    22501 aggcctgcaa tccgttctac aagggactga tgctgttcgg cgggggagaa cttgccggaa
    22561 tgatctgcgg caccagcaaa cctgtggtgc tgatgtcaag aagcgaaagc gagctgtcaa
    22621 aatactactg cgtggccctg gcatgcctta tggcctcaca aaaattatga taatgatgaa
    22681 ggagaagctt atccttgcta taaatcccgg ttccacaagt accaagtttg ctctttacga
    22741 gggtaaagac aaactcatgg agaagacgct gagacacagc actgaagagt ttgaaggctt
    22801 tactaccatg actgaccagc tgccgttcag gtaccgggta atcatgaatg cactcaggga
    22861 ggaacaggct gatctgaagg aaattgcagc ggtggttggc aggggaggac tggtgaatcc
    22921 tatcgagtca ggtatctacg aggtcaatga cctgatgaag aaacatcttt cagaaggggt
    22981 caatggggag catgcaagta acctcggagc gctgctggct gatatggttg tgaaagaact
    23041 cccgggagca aaagcatata ttgttgatcc cgtggtggtt gatgagctgg aaccaattgc
    23101 caggctttca ggacaccccc tgatatcaag aagatcgata ttccatgcgc tgaaccagaa
    23161 agctgttgcc agggtatatg cctcatcaat cggaagggag tatgaagatc ttaacctgat
    23221 tgtggcgcac atgggcggtg gtataagtgt cggagcgcat cgtcgcggaa gggttattga
    23281 tgtcaataat gcacttaacg gcgacggccc atactctccc gagcgaagcg gcggactgcc
    23341 ggcatggcag ctctccgagc tctgtttcag cggaaagtac aaaccttctg agatgaagtc
    23401 catgcttgcc ggaaagggtg gaatagtggc ttacctggga acaaacagtt tccaggatgc
    23461 gtcaaaaagg gcaaccgagg gtgacacaca tgcagccctg gttgtcgaag gatgcagcta
    23521 tcagacagct aaagagatag gagctatgtc agctgttctg gaagggaaga tcgattgcat
    23581 aatactcacc ggcggactgg catacgacag tgcccacgtg gagagggtga cccgcatggt
    23641 gaagcatatt gcccctgtct atgtctatcc gggtgaagac gagctgaagg ctctggcttt
    23701 caatggctac ctggccatca ccggacatat tatagttaag gaatacaggg gatagaagac
    23761 agcgttagga attgcgaatt gcgaaggatt tgcgaaggcc gaagtacgag ttgcgattac
    23821 ctggtggcgg ctgattacgg aacagacatg gctaccggaa ggatcttccc gttgaagaac
    23881 ctgttgccgt taacggcgaa gtcagctatg aaggcggcca tgtcggaagg caatacaggt
    23941 gcgctgaatc cgggaaacgc tttctgaaac atctccgtct gtaccgctcc gggacagaga
    24001 caattgaatg agatgcccct ttcggcatac tctgcagcga gacactccgt aagcacagcc
    24061 agggccccct tggtagcgct gtaccatgat aatccgggaa acttcagact gccctgaaag
    24121 ccgcccatgc tcccgatatt gacaacatgc gacccacgga ccattaacgg cagcagttct
    24181 gctgtcagcc tcgcggcgca ggtgaagttg acagacactg actcagctat gtcagcctcc
    24241 gtgtgatctt caaatggttt gtttacaagg tggccagcgt tgtttatcag tatgtccacc
    24301 tttcccgctt cccgttcaag ccttttttta agctcgctga gcgatgatcg tcctgaggtg
    24361 atgtccatgg gcacgggaat gatattcttg tgtccggcag cctctgaaag gcctgtaagc
    24421 gatttcctgt tgcgggcaat cgcatatatc ttgttacccg gctcctccga gagcctgatg
    24481 gctgtttccc ggccaattcc ggatgatgcc cctgtaatta ctatattcat cttattgtca
    24541 gtgctataac tttacccggc gttttttgcc tggcagaact accgtaaatg tacttaattt
    24601 tgattttatt tgtagcctca aaatgaggat actgcatcaa tgaagaagat actggcatta
    24661 gcttttttcc tggtgattgc tgcaacggcc gcaggacaga tcccggcgac ggagaatgct
    24721 gaggaggaga gacctccggt taatcagcgg ctctttttcg ggggatcgtt cggccttcag
    24781 ttcggcacgg ttaccaacat cgaggtggca cccctggcag gcatatggct gctgcccagg
    24841 ctggctgtcg gggccggacc gtcatttcag ttttacaaag atccatatgg aagaacctcc
    24901 atatacggag gcagggctat gctgcaactg actcttatac aggacctgaa caatgttata
    24961 cctatcggcc ttaataccgg gatctttgtc aacggtgagt atgaggcctt aagcctgcaa
    25021 cgcgccttct tcacctccga cccggaagct gacggtcgct tttatcatgg cactttcctg
    25081 gccggagcag gtttgtcaca gcccacaggc agacggtcaa gcatgaacat cagtttcctg
    25141 tggagcgtta ccggtaatga gtatggaatc tatgatactc cggagatcag gatagagttc
    25201 tatttttaat ttatataatt taattgaata taaataagat caaatagtta tgaaaatcga
    25261 aaacaacaag tttgcctccc tggcatatac tcttcacgag ggtgataagg gcggaagggt
    25321 gattgaaacg gtggaagagt cagccccgct ttcttttgtc tttggcgcgg gtcgtctgtt
    25381 gccggcattt gaagaaaagc tggttggact ggaagaagga gctgcatttg atttcagcct
    25441 tgctgctgct gacgcttatg gtgaggtacg tgaggaaatg gttatcagcc ttccgaggaa
    25501 tatatttgag gatgaaggtg ttctgagaag cgagatatgt tatgttggca acacggtccc
    25561 catgatggac tcgcagggga acaggatgaa cggtgtggtt gttgagattg gtgatgcctt
    25621 tgtgaagatg gatttcaatc atcccctggc aggcactgac cttcatttta ccggcaaggt
    25681 tgtaggtgtg cgcgaagcaa ctccggagga actgatgggt ccgtcatccg gcggctgttc
    25741 atcatgcggc agcaaaagca gcggttgcgg tggcagctgc ggatgagaac cagtcttcag
    25801 cagagagacc gcggccacta ctgccgactg gtactgccga ctatacaagg agctcttctc
    25861 cttccgactt ggcgataatt accgcaccgc aggtgtctcc gtatacattt atggcggtac
    25921 ggaacatgtc cagaatacgg tccacggcaa gtatcagccc tataccttca aggggcagtc
    25981 ccacagcatt cagcactaca gccatcatta ccagtcctgc catcggtatc cctgccgagc
    26041 cgatggctgc cagcagggct gtgaatatca ccaccatctg ctgcccgaaa ctaaggtcaa
    26101 tcccgtaagc ctgtgcaata aacatcacag ccacacattc atagagcgcc gttccgttca
    26161 tgttgatggt ggccccgaga ggcagggtga aacttgcaat cttgtttgaa acgccgttcc
    26221 tggcctcaac agtctccatg gtaagaggaa gtgtggcgtt ggatgaggat gtggagaaag
    26281 ctgtcagcaa aacggtggac atgttcttca gatgcttgac cgggttggcc cttcccagga
    26341 acctgaccag cagtggaagg gtcacaaaac cctgaaccac aagccccagc agcactgtca
    26401 gaaaataaat tcccatgctg ccgattatct ccccgacctg gttgccactg ttaatctgtt
    26461 gcgagagaac ctttgcgatt atgctgaata caccaagcgg ggtgaacttt attacgaaca
    26521 gggtgatctt catgatcaca tcaagaatgg cattgaccac atccaccaga aggacccgcg
    26581 acttttcacc cacacgcgtt acgaagaaac cggcgagaat ggcaaagaaa atgattgaaa
    26641 gcatctgtgc ctcagacatc gcacgtatga tattaaccgg aacgatgttt atcagggttt
    26701 gcccgaaact ctccttgcct gttgatattt catccagcgg cacctgcaat cccaggtcag
    26761 ccccgatccc cggttttatc agctgaacga aaaagaaccc ggtcagtatg gcgagaaagg
    26821 tggagaaaac ataatatccc atcgttttca gccccagcct tccaagattc tcggccgttc
    26881 ccacacttgc cacacctgtc atgatggaac agaatatgag cggcacgata accatgttca
    26941 atccccttag aaaaacgtca cctatccagt tggtgtattt tgtcagatga gggaagaagt
    27001 aaccgaataa gccgcccaga gccagcatga tcagtatctg ccagtgcatg gcaagtgaaa
    27061 atcttctttt taccatatgt cagtcgttat cccaggatac aggataaaaa atgatgtaac
    27121 ctgttaagcg tatgaagata attaaaagaa tcctcagcaa aatatgtttt aaattatgtt
    27181 tccagaatta aatattccct gccaggggaa aggggagctc aaaaaaatca attctcagca
    27241 acttcaccat ccacaagcaa atctcatcac ctggcaacct gtttatcttc atcctcagaa
    27301 ctaataagca acccgaatta cagatgtaaa caacccgaag ggggcttgac tataccaaag
    27361 taattactgt tgtaatgaaa gaaacatgaa gcatgtaaag aaacattgct ttgacatgca
    27421 gtttctatta ataaacataa tatcaatgca ttagcatact taattaaaca attaatgcac
    27481 cacggaaagg ccataagttg tgtatatgtt tagaataact catataatta tatctatata
    27541 actaattatc agtagttatg catatctaaa ctcaagtata catacaagta attatataat
    27601 gaattgcatc agtattcttc acggcttcca ggatttaata tctcagtgat cgcaatggat
    27661 tacatttgta atgaacatta aatgacatat gtaaattata atatcattac atttgtaaaa
    27721 acaggttcag gctatgaaag accgcataca ggcattcctg cagaatgaaa acaagagtta
    27781 tgcacagttt gccgatgaaa taggtgtgca gccgtcaggc atttcacata tactatcggg
    27841 aagaaataat cccagtcttg atttcgtaat caaaatgctg cacagatatc cctctctatc
    27901 cgctgattgg cttttgtttg gccgtggttc aatgtataag gtagtatcac agcctactct
    27961 ttttgatgta gatatgcaaa aggatcagcc agctgaaaat gaagtatttg ccgaacagga
    28021 tttagcggct gacaacgggg atattccgga tgtcaatgca agccggcagg atgtggcaga
    28081 agtattccag acagaaggga atgaaccgga aagcggttcc aggaagctct cgagaatcgt
    28141 ttttttctat tcagacaaaa cttttactga gtataagcca tcctgagaga ttcagtgcaa
    28201 aatgtctatt tttgcaatca aggaggactg atgaaaaaac agattgaaga tcttacggtt
    28261 atcggtaaca gcaagctcac cgaagggttc tttgtgataa aggtttcttc atcagaggat
    28321 ttgacaggga ttaaacctgg tcagttcgtt caggccctgg ttacggaaag tccttctaca
    28381 ttccttcgcc gtcctctctc agtacatgac attgatcccg gagaaaacac gatgtcgctc
    28441 ctggtgcaga ttgccggtcc cggtaccgaa aggctctccc ggataacccc tggtgaaaag
    28501 ctgaatctca tttacccgct tggcaattca ttcacccttc ccgcacgggg cgaaagagtc
    28561 ctcctggcag gtggcggatg cggcatggca cctcttctct acctgggcag gaagataagc
    28621 gaatcaggcg tgcagcctca ttttgccctc ggctttcgta acaggtcaag aattctcagc
    28681 cacgatgagt tcagtgctct gggaccggtc tttctggcca ccgaggacgg atcaaaggga
    28741 tacaggggct ttgtgactga tatcccggag ttcaggaatg aacattggga cagggtctat
    28801 tgttgcggtc cggaaccgat gatgaaggct gtggctgcac tgtgccggaa aagagatatc
    28861 ttctgcgagg tgtcgcttga aaacctcatg gcatgtggtc tcggaatatg cctgtgctgc
    28921 gtggtaccga ctacttccgg caacctttgc tcgtgcactg acggtccggt attcaacata
    28981 aacgatctca aatggcagac ctcaggataa agattggaca gctggagttc cgcaacccgg
    29041 tcatcctcgc atcgggtact tgcggctatg gctcagagct tgaggatttt atcaatctgt
    29101 ctgagctagg aggcatcatc gtaaagggaa ctaccctgga acacagggaa gggaatcctt
    29161 atccgcggat gtcggagaca gcctcgggaa tgctcaatgc tgtaggactg cagaacaagg
    29221 ggatcgacta tttcgaaaag aacatttatc ctcacctggc tgaatataag acacatgtga
    29281 ttgtcaacgt gaacggcaac accattgagg attacgttga tctctctcag cgcataaaca
    29341 ctctcagcgc catacctgcc attgaactca acatctcatg cccgaatgtc aaaaagggcg
    29401 gcatgatctt tggcaccaat cctgcctctg ccagggaagt catctatgcc gtacgccgcg
    29461 tttatgataa aaccctcatt gtaaagcttt caccaaacgt caccgacatc accgaatttg
    29521 ccaggatatc acaggaggag ggagcagaag cagtctcgct ggttaacacc ttccttggca
    29581 tggctgtcga tgtcaggact atgagaccga agctttccac aatcacggga ggactttcag
    29641 gtccggcgat caaacctgtg gctctgagga tggtgtggca ggttgcccgg gctgtcagca
    29701 ttcctgtcat agggctggga ggcatcatga atgcggagga tgcccttgag tttatcatgg
    29761 ccggagccac cgcggtacag gttggaacag cctcgttcat caatccccgt gcaggtacgg
    29821 agattgttgg tggcattgaa aagttccttg ttgaaaaaga aatggattca gtcagcaata
    29881 ttaccgggat aataaatagg atataagcca tatttacttt aaatttgtaa ttttaaaggc
    29941 ggaatattgg acatagactt aattaaggaa agtaatcatg gcagaaaatt tgcaaattga
    30001 caatctggac aggaagatcc tggacattat aaccaagaat gctaggatcc cgtatctgga
    30061 ggttgcgcgt gagtgcaatg tatcaggtgc ggcaatccat caaagggtcc agaggctgat
    30121 aagggtaggt gtgatcaagg gatcagaatt caaggttgat cccatcatgg taggattcag
    30181 aacctgcgct tatatcggtc tttatcttga taatccgggt aaatacaagg aagtgatcag
    30241 caaactgaag gatattgctg aagttattga atgtcactac accacaggca attactcgtt
    30301 gttcatcaag gtttacaccc gagacaatga gcatctgcgc gaggtgctga cagagaagat
    30361 ccaggccatc accggtgtga tgcgaacaga gacgcttatc tctctcgagg aatctatcag
    30421 caggcagata ccggtattat caaagtaata gagacagagc ttcaacctgc aaagggctgt
    30481 ccggactgac agcctttttt ttgtgccggc agggtgttac aatgcctgat cctctgcgag
    30541 cgaaaaggaa tgacccggtg ggtcattcca gcgaagcagc cagactgcag caggggcggt
    30601 tcgagcatat accagacaaa aaaaggcagc ttccgctgcc tccggtgatc cagctggggc
    30661 tcgaaccggg gcgagcgaaa aggaatgacc cggtgggtca ttccagcgaa gcagccagac
    30721 tgcagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncagac aaaaaaaggc
    30841 agcttccgct gcctccggtg atccagctgg ggctcgaacc caggacccca tccttaaaag
    30901 ggatgtgctc ta
//
LOCUS       DAOPXU010000026        29668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_1542_length_29668_cov_14.600209, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000026 DAOPXU010000000
VERSION     DAOPXU010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 29668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29668
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1542_length_29668_cov_14.600209"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="PKK03_04185"
     CDS             <1..1193
                     /locus_tag="PKK03_04185"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNX83626.1"
                     /translation="MSKFKLDALLDITLSINANLPTAELLRKYESILRDELGIGKILL
                     FKYGEQWEPLLNSGFPESLKASVDVENDLLCFREIQTEVVQKHFRAVDFIIPVLHNNE
                     PSAYVLIGDIDEEGEGMSPLIKHLNFIQTISGIIVVAIENQRLFRESLRQEALRRELE
                     LAARMQMMLIPDNTRLPSDNLIRAKGFYYPHYEVGGDYYDFMQLSENVAGFCIADVSG
                     KGMSAALLMSNFQASLRALFTAGASLEELIRTLNTLVVTNSAGERFITLFIARYNTET
                     RELEYINAAHNPPVIINTRTGQSSLLRQSCVGVGMLDEIPCVRTETLTLKNPTKIICY
                     TDGLSELKDDGGRDYGIAPLVKHFPNGKPLAENIESLIRDIDISSNNQRLFDDVSILA
                     VEIP"
     gene            complement(1236..2618)
                     /locus_tag="PKK03_04190"
     CDS             complement(1236..2618)
                     /locus_tag="PKK03_04190"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNX83627.1"
                     /translation="MTGNELLILIIPAVLAVLLLALLSLEHLLLLTLFLAPLSLQLSY
                     LTGSTGFDLSVPTEPVMALLLLITLFRLTVTRGFPKELLRHPMTWMVGLYLLWTFITA
                     LTSTMPGVSFKTLAYRLWFTAGFYLVAARLAYDERFRKRYILAYATGLAVVVIYFIVR
                     VQGAGLLNQQFAHSACYPFYKDHTSFGASMAFILPPLTVMLFSRGSLWHRGFLAALLL
                     IFVAGFVLSYSRAAWVSLIAAAALALILWIRMPVRMLSVSAALFVVVLALSAGWIWQK
                     MDSTTEDSSTDLGQHLRSSSNISTDQSNLERINRWKCALRMFAEKPNTGWGPGTYQFR
                     YAPFQKSSERTAISTDFGDAGNSHSEYLGALSESGWPGALIYLAMALCAVITGIKVWY
                     REGRGDKGYFALAVVTGLVTYAVHGVMNSFLDSDKIAALWWGFIALIVAMDLEGRIMA
                     GATESDSNPS"
     gene            complement(2605..3492)
                     /locus_tag="PKK03_04195"
     CDS             complement(2605..3492)
                     /locus_tag="PKK03_04195"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNX83628.1"
                     /translation="MKGNIRAEFRQDSFDLLNFFIRHFRKFVTAGIAAAILSAVISLL
                     IRPLYESTVILYPSSNIAEATSLLGETESRTALFGDDDATEKLIQVINSGQVREYLKE
                     KYDLVQHYNIKPGEKYPNTLMKAKMDKYLHCTRTNYGSVEIRVRDRDREYACNMANDM
                     ATRADTIFNDLQRDAAMVTLDEISRLYDIQDRLVRQYEDTLTSLSGAAALRLYSTLET
                     ENDYLGLMRGRFLEAQALSRQTLPYTLVVDRAVVAEKKVFPRRSIMVLLSTASVLLLL
                     ALILFIAEGVKLRRADDRQ"
     gene            complement(3530..4828)
                     /gene="tyrS"
                     /locus_tag="PKK03_04200"
     CDS             complement(3530..4828)
                     /gene="tyrS"
                     /locus_tag="PKK03_04200"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNX83629.1"
                     /translation="MNFVEELRWRGMIHDIMPGTEEFLLKGKTSAYVGFDPTADSLHI
                     GHLVGVMMLKHFQNAGHTPIVLIGGATGMIGDPSGKSEERNLLDEQTLRHNQECLKKQ
                     FEKFLDFNSAASNRAVMVNNYDWMKEYSFLGFIREIGKHITVNYMMAKDSVKKRLDSE
                     TGQGMSFTEFSYQLVQGTDYLFLYTNHGCQLQMGGSDQWGNIVTGTELIRRKAGGEAY
                     AMTCPLITKSDGQKFGKTETGNVWLDPTRTTPYQFYQFWLNVADADAEKFIKIFTMLP
                     QGEIAGLVKAHQDEPHNRILQKRLAEEVTVMAHSREDYEAAVEASQILFGKGTTEQLR
                     KMDENTFLAVFEGVPTFDISAGLLETGVNFSDLCAVHTAITESKGEFRRLIQGGGVSL
                     NKGKVDNAEMIITPEHLLNNKYLLIQKGKKSYFLIRAKEN"
     gene            complement(4873..5409)
                     /locus_tag="PKK03_04205"
     CDS             complement(4873..5409)
                     /locus_tag="PKK03_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNX83630.1"
                     /translation="MNWIDFVIIALLAFGLIQGFIDGLIIEIAELAALVLGIWGAIHF
                     SWWTADKLAGWFDMNSSWTGVISFAVTFIVIVTVVYLVGKILDSVMKAISLGFINKTL
                     GAVFGVLKTALILSVIFVFLNTIDEKKHFLPSATINNSFFYNPIADLVPSIFPVIEGG
                     DLIDSFNRHKKQKGDVTI"
     gene            5537..6604
                     /locus_tag="PKK03_04210"
     CDS             5537..6604
                     /locus_tag="PKK03_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83631.1"
                     /translation="MDLQFTDVIAVFDIGRSWKRFRLFDRSLNPVHTEEKIIAEAKDA
                     NGSPCEDIQAIAAWMETCLRNVSATSLYNIRALNLAAGDDCLAKLESSLTHSLKSGIA
                     GTSLFKGKGMNSTAASLIPYLKGSDKPFILVSTGTWCTFMNPFDHESGSVTHAGNGKT
                     AGKPGENTGGMEMDAAITVGERQIKSSQFPLGEVHDRNVEMLDDHFGVTGELYKTIKI
                     KHKKILKMQANRRGRVFFRHGIPAGQADTLADLSHFLTYADAYHQMMYDLTDECLMAY
                     REIIAEGDTTEIAYITGGFAHNDTFVRILAARAEDKRVYASTIDYATALGAAMEVYQQ
                     AFDAGLPPVYLGLKAILLRDE"
     gene            6695..9514
                     /locus_tag="PKK03_04215"
     CDS             6695..9514
                     /locus_tag="PKK03_04215"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNX83632.1"
                     /translation="MNRPDSFMKLILVTLAVIFITACGKTTQDDKLSLSYEKFIMPNG
                     LQVVLHQDHSDPVISYAIMYHVGSSRETPGRTGFAHLFEHLLFSGSENVPNGVFDKVI
                     ENAGGSNNGFTSRDVTAYFESFPKNALEKILWLESDRMGFFINSVTPRNLAIQQNVVQ
                     NEKRQGEDNSPYGFTNYVISKNLYPASHPYSWEVIGEMEDLTNATVDDVRGFYDNFYG
                     PNNATLVLAGDFEPDSVRLLLDKYFGEIKPRGEVAARKAMPVTLSESIRLFHEDNFAN
                     VPEINLVWPVPENYTRDSYALSFLAEILASGKKAPMYKVLVKEKQLTSRVSAYNGAEE
                     LAGEFAISATANEGRTLKEIEDAIFEAFARFENEGFTDREVDRIKASMEKGFYQGLTS
                     VLNKAIQMASYNTLLNDPGYVGKDIENIKAVTREDIMRVYETYIKGKPHIVTSFVPRG
                     QPEMMAENSVKAVVSEEDITKASQVAINDILETEIAKTPSVIDRTKEPPSGPEPEVRV
                     PEVWKATLKNGIRVYGTVNRELPLVSMSVVIAGGALVDDIAMPGVSSMVAAVLPQGTR
                     NKTPEELEEEIQLLGSSVYMRASGEEIVFSGSTLSRNFGKTVSLMKEILLEPRWDTAE
                     FRMAQTRTRNALIQSEARPTSVASDRFMKLVYGPGNIFGYDIRGTKESIELITPDILK
                     DFYERNFSPSVTSVYVAGNVSKDEVLEALKPLEAEWHDGEVAFKSYEMPAAPEKSQIY
                     FVDIPGSRQSVVYAGYLAMTRDNPDYVKADFANYRMGGAFTSILNQILREQKGFTYGA
                     HSSFREMKIPAPFIISTSVRSDATLETLEIITGELGKYREGIPEEELQFIKNSMIRSN
                     ALRFETNDALADMLTYIGKYGFSDDWVKKEEDVIRNMTVEDHKAITQKYFDPDRMYYV
                     VVGDAATQMKQLEKVGFGTPVLLAR"
     gene            complement(9563..9985)
                     /locus_tag="PKK03_04220"
     CDS             complement(9563..9985)
                     /locus_tag="PKK03_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HNX83633.1"
                     /translation="MNKPLLNDPGIPPSEELLKSLLGASYHAYDEMMKAITAAEYGLT
                     PEWRFYNDGKAWLCKVVFKKKTVFWLSVWDGFFKAGFYFVERHCPGIGELGIDPGITE
                     ALSEAKPFGTMYPVTLEMRRKEQIGDLLRLVEYKKKLK"
     gene            complement(10059..10670)
                     /locus_tag="PKK03_04225"
     CDS             complement(10059..10670)
                     /locus_tag="PKK03_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNX83634.1"
                     /translation="MKKITSVVFDIGGVLIDWNPRHLFRKVFENEEEMEWFLANVCTY
                     EWNVQQDGGKLFSVATAELQQKFPEYSDKIALYYGRWEEMLDGEIIGTVEIFRRLKSA
                     GMPLYALSNWSHEAFPVAYNRYGFMKEFDGLVVSGYEKLLKPDHAIYRVLMDRYSINP
                     AESVYIDDNKPNAIAATELGFNAIHFSSPDQLRGELALLGLEV"
     gene            complement(10738..11550)
                     /locus_tag="PKK03_04230"
     CDS             complement(10738..11550)
                     /locus_tag="PKK03_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83635.1"
                     /translation="MAQRKLIMLVVAVMSLTSVSLPGQNIGLTADRPDVGRKDTITVT
                     VKAADVNGEPVQALITIYAVHSRRSLHKGPSGGSGGYPGTNVSHNDVFSMIQQIKPFR
                     LTDGRIIFTSVAPDTNGRQDGALIVVDGIMLGQDPRVIENMNPSDIDRINVSTEFVDI
                     QRYTGFNTTGVIEIWTKTGRIRSGRTVKTGESGTEINSDGAEIPGNAADSIASARSGK
                     VILRGMEVRTDVSGKAHLDIPSGKKSGEITIIAEYRSASGTLKNETATVRVR"
     gene            complement(11532..13106)
                     /locus_tag="PKK03_04235"
     CDS             complement(11532..13106)
                     /locus_tag="PKK03_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013064136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HNX83636.1"
                     /translation="MKTTVNLIVISVIMLFAGCGGDRLSDATATVKGGVVQGTVEDGI
                     AVFRGVPFAAPPVGDLRWKAPQPVRPWDGVLTADKFAPASIQPAVAWMGGRPTSEDCL
                     YLNVWTPAKSAKEKLPVMVWIYGGGFAFGGTAGADTWGDQIARHGVILVSIAYRVGVL
                     GFMAHPDLTAESENKVSGNYGLLDQIAGLKWVQENISAFGGDPARVTVFGESAGAISV
                     SMLCASPLTKGLFAGAISQSGGSFGPVGDNSGSGDFMQTLAGAELAGMEFAKIMGANS
                     IDELRAMSPDKWLSDPRSQMGGFWPVVDGYVITDDQYKLYEAGNYNDVNVIIGTNSDE
                     GSMFTQPVKPEEYAGVLKMRFGPLAEKALQLYPGNTEYETYASMSDIFRDAVFGWPSW
                     TWARLQSKTGKSRVYVYYFDQFRKEPLFPGGSPQRGAAHASEIPYVFGHLNQNPDAKA
                     TEEEIILSDRMIKYWTNFAKNGDPNGEGLPGWPVFEEGKETVMYLKGSVPLPIGVPNM
                     DKLLLMEEYYKWLREN"
     gene            complement(13118..14827)
                     /locus_tag="PKK03_04240"
     CDS             complement(13118..14827)
                     /locus_tag="PKK03_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNX83637.1"
                     /translation="MNIITSTMRLFILMSVVFTCFSGQAGAQRLKSGPQDLSFFSSVD
                     DTDQPYSVYIPENFDESKSYPLVVFLHGAWSNHRLGMRRLFGVGNAQGYDFIKPGNVA
                     FENDVEASRYWPPFRPVDYIAAAPLARGTAGYQGIPEQDVYDMIDDLKARFHIDEDRV
                     YLTGLSMGGGGTLWLGLTRPDVWAAIAPVCPAPPEGTMELAGNASNLPVHLFIGDKDF
                     LFQTATEWKVKLGVTAMRLDYVEYPGVGHNSWEWAYKDGFIFDWFSQFKRDLYPGKVS
                     FTTKWFKYNSAYWVTIDDMIPGKTATAEAKFTGPNSVAVTVTGINAFTLRLDGHPQFS
                     PSKKVSVTVNGKTFSIRSADAVSFLLSEGNWVNTRFTPGLTSKQKGAEGPLYAAVAGS
                     HIYVYGTADNPPADVLAARRAQAMAAADWTGMGGRIMVFPRVVADRDVRSSDFVSSNL
                     ILFGTRETNSVIEKFADKLPMHLDPKAENYGLVYIFPMNRHYILVNSGLPWWTPPKGK
                     TAQAGLAFSGTNVEILKKYQDFILFKETPDYIITQGSFDNNWKLPADAAEALKGVATI
                     KLN"
     gene            complement(14913..15347)
                     /gene="fucU"
                     /locus_tag="PKK03_04245"
     CDS             complement(14913..15347)
                     /gene="fucU"
                     /locus_tag="PKK03_04245"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HNX83638.1"
                     /translation="MLKGISPLISPDLLDVLARMGHGDELVLADAHFPGESIGKRVVR
                     ADGLRIADLLEAIMPLFELDSYVPHPVMMMEPVPGDQADPFVEDSYLEQIRKSAPGVP
                     PVKKIDRFAFYERASKAFAVVMTGETVKYGNIILIKGVTPVI"
     gene            15516..16724
                     /locus_tag="PKK03_04250"
     CDS             15516..16724
                     /locus_tag="PKK03_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNX83639.1"
                     /translation="MNNDRREFLKKGLLGVSAAAFIPGVIKGSPAAAEASASLPDLPS
                     RVLGRTGIQAPLISLGASGAITPSFIKGAYNAGIKLFFSATYYGEGNNEIIVGEALKG
                     FPRDSFVVATAAPCNEVDNRTGLLTDKFSARAYMEKAEGSLRRFGLDHIDIILFPYAS
                     KRETVCNDEILKTMEQLKQQGKVRFAGIASHGGIPEALDAAASTGIYDIAMISYNFKS
                     ENREALDTAIAKASASGMGIAAMKTTAGAFRNKSGPGINSDAALKWALQNVNISTIVS
                     GMTTAEQIQKNLAMIKNLKMTEEEMADLNTALAFSETGLFCQQCGHCLPQCPASLDIP
                     TIMRGYMYAYGYRNLPQARNTLAASGLKSGPCADCNSCSVKCSSGFDVRGKIQDIARL
                     IKVPEDFLLT"
     gene            16831..17637
                     /locus_tag="PKK03_04255"
     CDS             16831..17637
                     /locus_tag="PKK03_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNX83640.1"
                     /translation="MKPTDFFTLANGVAIPAIGFGTWQIPSGDIAYNSVKEALKAGYR
                     HIDTAQAYGNEASVGKAILDSGIPRREIFVTSKLPAEIKSYDKAVLHAELTMKNIGLD
                     YIDLYLIHAPWPWTEIGANYTKENIDVWKAMEEFYESRKTRSIGISNFNVKDTEAILG
                     TCRIRPMVNQIKLHIGHSQEEITEFCRKNNILIEGYSPLATGKILNNKEIARIAEKYG
                     RTPAQVSVRYVLQKGALPLPKSVHPGYIRQNFDVNFEISNDDIRLLDSLK"
     gene            17677..18474
                     /locus_tag="PKK03_04260"
     CDS             17677..18474
                     /locus_tag="PKK03_04260"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX83641.1"
                     /translation="MLPEARYYKPAEKLSWLIKQFEFITYNGKEALTDKFVPREDVSI
                     VFHFRNRPRMVSPVDQTLPPVFIAPLTPTANSICLPGGNDSMIVTCRPTVLSRMFGIS
                     LSPGNKIWVPVPEGLLLPLWEEMKQGKSDAARMDVFASFISSNWHRRYIPDETDRSYD
                     LILRKGINTNLHDITDELQVSERTLQRRFRDRLGATPKMLIRLIRINYLWETTSGGGK
                     IDYQDLVFLGSYFDQTHMIKDFKSITGETPKAFFRRNLSMARIFSGK"
     gene            18570..19886
                     /locus_tag="PKK03_04265"
     CDS             18570..19886
                     /locus_tag="PKK03_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX83642.1"
                     /translation="MKIRVFFSIAVILSFLLSAQSVNAQIVNPKDVVKEKSTNRINTR
                     IDQGVDEGFNKIEEGVGSLFKKKKVKSGEEAEAEENEPDTDTEAEEPEAERQKDAAPQ
                     KQKLESFTQYDFVPGDRILYFEDFSQDAIGDFPALWTSNGSGEVKSVNIAPGKWLHMN
                     GEDAVYCYSKTVAFPDNFIVEFDIIPDEEYGRGIVFTMYEENPEDPKEINDDLYPGKS
                     GFHVILESGGWETKGYTNDPEGDWIEGQASRNPVTTEEVNHVIIWIQKRRARIYHQGA
                     KVLDVPTNIYAHTKFNKILFSGWDRASWPMVTNIRITSAAPDTRSKLITEGRLTTYGI
                     TFDVNKADIRPESRGTLNDIATVLKENPDVKVKIIGHTDSDGDDALNLDLSRRRAESV
                     RTELSSKFGIDASRMQTEGAGETKPVAPNDTPANKAMNRRVEFIKQ"
     gene            19943..20626
                     /locus_tag="PKK03_04270"
     CDS             19943..20626
                     /locus_tag="PKK03_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83643.1"
                     /translation="MMIKRIIAFAVLLLTAGTMTAQNCVFYFPSEVGTRVGYNYYSKP
                     GKLEGSSTLLVTDRSTENGNLKLDIKATVFDTHEDTTITFDYAVWCDGDNFYIDMRSF
                     LGSMNITELGEFKITSADMVLPAGMSPGQQLKDASFTLEMTSPIPVTMTSNITNRKVD
                     AIEQVTTPAGTFECVKVSYDTFSKVAFIKTEGRTVEWYTPNIGIVRSEYYDKKGRVTS
                     IHELSYVKK"
     gene            20694..22796
                     /locus_tag="PKK03_04275"
     CDS             20694..22796
                     /locus_tag="PKK03_04275"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNX83644.1"
                     /translation="MITPARKRTLSLLTNPFFLAAIPSIALFFIIPVIHQRYRLDLKS
                     STLVPEGYYHSYCDLDGDGNSEKIVAIEEENASGITISRDNRVLNQWNVRGSFSFSNR
                     NTIYIPADCNTDGLKEIYLFSLSGDSVLLNCIPGPETNKFSVANMLVDVVGHGIKAPD
                     PSIVAAEPEDLDGDGYPELIFGISTGFSKHPRKVYAYNTVTETIISSPQCGGFMLGIV
                     QHDLNNDGFMEIMPYGYASENIGRDEMKYHDYSAWLTVLDRNLQFVFDPVEFGGRYTM
                     ALPMIHPLSVDTVAGLLVSRGEPDSTSVFRYFSMHGLLPGRTPVRSSAHNGMITVNRK
                     GEPLYALVDYDRGIFILDEQKQLKKTMEFTGLRQLKTMDLDRDGSEEILVVSLGERMA
                     TVFRSGFIRPAMADLGNELRGESVASVICRKGEFPQVFLQSDTRQFVLEYRPNPFYFA
                     SFVFYPLVYAAFIAFVLLIQFSQRKLIARREDEKKKITELQMALLRNQLDPHFTLNVL
                     SSVLSMVELSEKEKARDSLLRFSGLYRELLLSAGKSRRTLAEELEFCRGYLALEKMRY
                     LNRFDYMVDVQPGINEELPVPKLVIQLFAENSVKHGLAGLDSGGLLGINVTGTGNELT
                     IEVKDNGVGRAHAVNDHSSSTGRGIKLMSDLFDMCNGYFEEAHSFTVSDLSDSEGKPS
                     GTLVTINIIYRHQSVFLS"
     gene            complement(22856..22927)
                     /locus_tag="PKK03_04280"
     tRNA            complement(22856..22927)
                     /locus_tag="PKK03_04280"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22892..22894),aa:Arg,seq:cct)
     gene            complement(22977..23600)
                     /locus_tag="PKK03_04285"
     CDS             complement(22977..23600)
                     /locus_tag="PKK03_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNX83645.1"
                     /translation="MKPLRLLALFVIPLGIIIAAVPPDRVKHFRLTADELLQEAGAGV
                     QFVSPDVVADMLVQKDPSLRLIDVRTPEEFDAYHMEGAINIPLSSLLSDEYTDILHQD
                     AYMNVLYSNGSVYANQAWMITRQLGYENNFVLEGGLNYWFGNILSPQAPSQTSPNEEF
                     AKYDFRKSAAAALGGGNIVATEGGATAVKAVKPPVASKAKKKPSGGC"
     gene            complement(23674..24264)
                     /locus_tag="PKK03_04290"
     CDS             complement(23674..24264)
                     /locus_tag="PKK03_04290"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNX83646.1"
                     /translation="MGPLAPNGIISENTNFLLAFFIGIAFGWVLESSGFSSSRKLAGI
                     FYGYDTVVLKVFFTGAITCMLGLLFMSLFGWVDLDLIYVNPTYLTSAIIGGVVMGAGF
                     IMGGFCPGTSFCGAAIGKIDGMVFVAGLFIGVYGFAFTYPLWEKMYMARYLGEPKVSE
                     KLGMSDGLFGLLLIIAAIGMFAVAEWAERKFPREEY"
     gene            complement(24281..24829)
                     /locus_tag="PKK03_04295"
     CDS             complement(24281..24829)
                     /locus_tag="PKK03_04295"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNX83647.1"
                     /translation="MQHKMSNNTTNERSRRYLNPYLGGVLLGLVLLAANFVSGRGLGA
                     SGAVKSVVATTIEAVDRNASETMPFMKEYAGSHSGSPMKSWLVFQMLGVIVGGFLSGA
                     VAGRLKLKVEHSPKITSRRRLIFALAGGILFGFGSQLGRGCTSGSALSGMGVLSLGGF
                     VTMMAIFGTAFALAYFFRRNWI"
     gene            complement(24840..25781)
                     /gene="nrfD"
                     /locus_tag="PKK03_04300"
     CDS             complement(24840..25781)
                     /gene="nrfD"
                     /locus_tag="PKK03_04300"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNX83648.1"
                     /translation="MNEELIISGRENLMVDPQLHIWHWPIPLYLFLGGLAAGVLFFAA
                     LYYLMGKENEYRSAVRRVPFLAPIALVLGLAALFYDLRHKAFFWQLYTTIRLESPMSW
                     GAWTLLVITTASFIWCALHIREFFPGWDWKYNWLKDLEVFFNKYKKQLAWVILIFAII
                     TGVYTGILLSAFNARPLWNTSVLGPLFLASGLSAGAAAIVLMSKNREERLLFTKIDLI
                     ILGVELFLIVHMFMGFLASTQVQIEAAELFLGGPYTMVFWIFVVILGIIIPGVLDLME
                     LRGKNIPAVIPGVLVLLGSFMFRFIFVFAGQVSRWLY"
     gene            complement(25789..26322)
                     /locus_tag="PKK03_04305"
     CDS             complement(25789..26322)
                     /locus_tag="PKK03_04305"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNX83649.1"
                     /translation="MRYAMAVDTKKCVGCSDCVVACQTENNVPIGYCRDWVVEVMDGT
                     YPQLEIELRSERCNHCENAPCVRCCPTGASHIVDGGIVLVKPNKCIGCGACIASCPYD
                     ARYPHPKGYVDKCTFCVHRVSQGMKPACVSVCPTKCMYFGDLDDPNSDVSMVLKKRKY
                     KTLIPEAGTKPHLYFLI"
     gene            complement(26319..28556)
                     /locus_tag="PKK03_04310"
     CDS             complement(26319..28556)
                     /locus_tag="PKK03_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNX83650.1"
                     /translation="MATRRQFIRISAMGAGAVMAGAAGLRLLASEITDGRKASYVALT
                     GRTPTYCEVCFWKCAGWVYRDDKGSIQKIIGNSEDLNCNGRLCPRGTGGVGMYHDEDR
                     LRKPLIRTEERGMQSFREATWEEAFDYIAEKIKVISAEHGPECIALFTHGSGGKYFST
                     LLRALGSPHIAAPSYAQCRGPREVAFQTTFGEALDSPEPLDIRDTRCLVLIGSHLGEN
                     MHNGQVQEMSDAIDKGATIITVDPRFSTAAGKSKYWLPIKPATDLALLLAWINVIITE
                     ELYDKPYVEKYTYGFEYLKEHVKNMTPEWAYGITTIDPTVIRETAREMANASPAVIVH
                     PGRHVTWYGDDTQRLRAVAILNALLGSWGRRGGFFRTETFELPEPPHPAYPTPKRTWR
                     TEFPDLYKLADTVLANGVCDATVPDPSRNCKFQGWIVNGTNLMETLPNQANTLKAIQE
                     LQLLVVIDTMPMEITGWADVVLPECTYLERYDDLRVGPHRKPQIALRAPAAKPMHDTK
                     PAFYMARELAKRLGVIEYFPFEKMEDQLDWQLKQVGSSLEEMKKIGVKTFDRTADNLF
                     FYPDEDVEFYTATGKIELYSTALEGAGFDPMPKYTAHEEPPAGYYRLNYGRAPMHTFS
                     RTANNPNLTDLMDENSVWVNPRVAKEWGLSTGQYVHLVNQDGKRSEFPIKVRVTERIR
                     WDSVYMVHGFGHQEKKLRRSYGKGASDTNLITKVLVDPVMGGTGMRGNFVTFITDLAG
                     EEVVS"
     gene            complement(28710..>29668)
                     /locus_tag="PKK03_04315"
     CDS             complement(28710..>29668)
                     /locus_tag="PKK03_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX83651.1"
                     /translation="GEGIPWKGNYTSWLEQKAKRLELEEKGSTKRRKTLERELEWVRM
                     SPKARHAKSKARLSAYENLLNQDVKQKEEKLEIFIPNGPRLGDKVITARGVTKAFGEK
                     ILFEDLDFSLPPNGIVGVIGPNGAGKTTLFRMIMGQETTDRGTFEVGGTVTLGYVDQA
                     HAAIDPKKSVYEVISGGQDEILVGNRPVNSRAYAARFNFTGADQEKKCGVLSGGERNR
                     LHLALTLKSGANVLLLDEPTNDIDVNTLRALEEGLDNFAGCAVIISHDRWFLDRVATH
                     ILAFEGDSEVVWFEGSFSEYDENLRRRTGNAGPVRFRYKKLS"
BASE COUNT         7387 a         7845 c         7382 g         7054 t
ORIGIN      
        1 agatgtcgaa gttcaagctc gacgccctgc ttgacattac cctctccatc aacgctaacc
       61 ttcctacagc cgagctgctc agaaaatacg agtcaatcct ccgtgatgaa ctgggtattg
      121 gcaaaatcct gcttttcaaa tacggcgaac agtgggagcc cctgctcaat tcaggattcc
      181 ccgaatcact gaaagcgtct gtcgacgtcg agaatgacct gctctgtttc agggagatac
      241 agaccgaggt ggtgcagaag cacttcaggg ccgttgactt catcattccc gttcttcaca
      301 acaacgaacc ctcggcctat gtcctgattg gtgacatcga tgaggaggga gaagggatga
      361 gtccgcttat aaagcacctt aacttcatcc agactatttc cggcatcatc gttgttgcca
      421 ttgagaacca gaggctattc agggagagcc tccggcagga agcccttcgc agggagctcg
      481 agcttgctgc ccggatgcaa atgatgctta tccctgacaa caccaggctc ccttcagaca
      541 atctcatcag ggcaaaaggc ttctattatc ctcattatga ggtcggcggt gattactacg
      601 atttcatgca gctgtcagag aacgttgcag gattctgtat agccgacgtc tcagggaagg
      661 gaatgtctgc cgccctgctg atgtcaaatt tccaggccag cctccgtgcc ctcttcaccg
      721 caggagcaag cctggaggaa cttatcagga cactgaacac tctggtggtc accaactcgg
      781 ccggagaacg gttcatcacc ctgttcattg cccgttacaa taccgaaact cgggaactgg
      841 agtatatcaa tgctgcccat aacccgccgg ttattatcaa tacccgcacc gggcagtcat
      901 cgcttctcag gcagtcatgc gttggagtgg gcatgctcga tgagatcccc tgtgtgagaa
      961 cagaaaccct gacacttaag aatcccacca agatcatctg ttacaccgac ggactctctg
     1021 agcttaagga cgatggaggc agggactacg ggattgcccc cctggtgaag cattttccca
     1081 acgggaagcc gctggcagaa aatattgaat cactcatcag ggatatagac ataagcagca
     1141 acaaccagcg gctcttcgat gatgtctcaa tccttgcggt tgagatcccc tgattttttc
     1201 tgaatgttta tcctgccgga catctgaccg gaaacctacg acgggttgct gtcactttcc
     1261 gtggcgccgg ccattattct tccctccagg tccatcgcta caataagtgc aatgaatccc
     1321 caccacaggg ctgctatctt atcactgtcg aggaagctgt tcataacccc gtgaacagcg
     1381 taggtcacca gtcccgtgac cacagccagg gcaaaatacc ccttatcccc tctcccttcg
     1441 cggtaccaca cctttattcc ggttatgaca gcgcaaagag ccattgccag gtatatcagc
     1501 gcccccggcc agcctgattc gctcaaggcg ccgagatatt cgctgtggga attgcctgca
     1561 tcgccgaagt cagtgcttat tgccgtccgc tcagacgatt tctgaaaagg tgcataccgg
     1621 aactggtacg ttccgggacc ccagcccgta ttgggtttct ccgcgaacat cctcagcgcg
     1681 catttccacc tgttgatacg ttcaaggttt gactggtcgg tggaaatatt tgatgacgac
     1741 cggagatgct gacccaggtc cgtggatgag tcttccgtgg tgctgtccat cttctgccag
     1801 atccagccgg cagacagcgc caggactaca acgaacaggg cagctgatac tgacagcatc
     1861 ctcactggca tcctgatcca caggatcagc gccagtgccg ccgcagctat caggctcacc
     1921 caggcggcac gactgtatga caggacaaag cctgcaacga agatcagcag aagggctgcc
     1981 aggaaacccc tgtgccacag gctgcccctg gaaaagagca tcactgtcag cggaggaagg
     2041 atgaaagcca tcgaggcgcc aaatgaggta tggtctttat aaaacgggta acaggccgaa
     2101 tgggcaaatt gctgattgag cagtcccgcc ccctgtaccc tgacgatgaa gtagataacc
     2161 accaccgcca gcccggttgc ataggccagg atgtatcttt ttctgaaccg ttcatcatat
     2221 gcaagccggg ctgctacaag gtaaaaacct gccgtgaacc acaaccggta tgccagggtc
     2281 ttgaatgaga ctcccggcat tgtggatgtc agggctgtta tgaatgtcca taacaggtac
     2341 agacccacca tccaggtcat gggatgcctc agcagctcct tcggaaatcc tcgtgtaacc
     2401 gtaagccgga acagggttat aagcaacagc agggccatga ccggctcggt ggggactgac
     2461 aggtcaaagc cggtgctccc tgtcagataa ctcagctgaa gagagagggg cgccaggaaa
     2521 agcgtaagaa gaaggaggtg ctcaagcgag agcagcgcca gcagcagcac ggcaagcact
     2581 gccgggatga tcagtatcag aagttcattg cctgtcatcg gcacgccgga gcttcacccc
     2641 ctcggctatg aacaggatca gggccagcag caacaacacc gaggctgttg acagcagcac
     2701 catgatgctt cgtctcggaa acaccttctt ctctgccacc acggccctgt caacaacaag
     2761 ggtgtaggga agagtctggc ggctcagcgc ctgagcttcc agaaaacggc cgcgcatcag
     2821 acccaggtaa tcattttcag tctcgagggt ggagtacagc ctcagtgcag cagctccgct
     2881 gaggctcgta agggtatctt cgtactgcct caccaggcgg tcctggatat catagaggcg
     2941 gcttatctca tccagcgtca ccattgcagc atcacgctgc aggtcattga agatggtgtc
     3001 agccctggtg gccatatcat ttgccatgtt gcaggcatat tcccggtcgc ggtcgcggac
     3061 ccgtatctcc actgacccgt aatttgtacg ggtacagtgc aggtacttgt ccatctttgc
     3121 tttcatgaga gtattcgggt atttttcacc cggcttgata ttgtaatgct gcaccaggtc
     3181 atacttctcc ttcagatact ccctgacctg cccgctgttg atcacctgaa tgagtttctc
     3241 ggtggcatca tcgtcaccga agagagcagt gcgcgattca gtctcgccaa gcagggaggt
     3301 ggcctcggca atgtttgagg agggatagag aatgactgtc gattcgtaca atggcctgat
     3361 cagcagtgat ataacagctg acagaatggc ggcggcaatg ccggctgtca caaacttcct
     3421 gaaatgcctg atgaagaagt ttaacaggtc aaatgagtcc tgtctgaatt ctgccctgat
     3481 attgcccttc atgctgcttc cgggtttttt atgattcttg ttccggtaat tagttttcct
     3541 ttgcccttat caggaagtaa ctcttcttcc ctttctggat gaggaggtac ttattgttga
     3601 gaaggtgttc cggcgtaatg atcatctccg cattgtcaac cttgcccttg ttgaggctca
     3661 cgcccccgcc ctggataagg cggcggaact caccctttga ttcggtgatg gcggtatgta
     3721 ccgcacagag atcagagaag ttcactcccg tttcaagcag acctgcactg atatcgaacg
     3781 tcggaactcc ctcaaaaact gcaaggaaag tattttcatc catcttcctg agctgctcgg
     3841 ttgtcccttt tccgaaaaga atctgtgagg cttccacggc ggcttcatag tcctctctgg
     3901 agtgagccat cactgtcacc tcttcggcaa ggcgcttttg caggatacga ttgtggggtt
     3961 catcctggtg agcttttacc agtccggcta tttcgccctg cggtaacatc gtgaatatct
     4021 taatgaattt ttctgcgtcg gcgtcagcca cgttgagcca gaactggtag aactggtaag
     4081 gtgtggttct tgtcgggtca agccatacat tgcctgtttc tgtcttgccg aacttctggc
     4141 cgtcactctt ggtgatcagc gggcaggtca tggcataagc ctctcctcct gccttgcgcc
     4201 tgatcagctc tgtgccggtg acaatgtttc cccattggtc agatccgccc atctgcagct
     4261 ggcatccgtg gttggtgtac aggaaaaggt aatcggtacc ctgaaccagc tggtatgaaa
     4321 actcggtgaa tgacatcccc tggccggtct ccgagtcaag cctctttttt acggagtcct
     4381 tggccatcat gtagttgaca gtgatgtgtt tgccgatctc ccggatgaag ccgaggaacg
     4441 agtattcctt catccagtca tagttgttga ccattaccgc cctgttcgat gcggcggaat
     4501 tgaagtcgag gaatttttca aactgtttct tcaggcactc ctggttatgg cgcagggtct
     4561 gctcatcaag caggtttcgc tcctctgatt tgccggaggg gtcaccaatc atacccgtgg
     4621 ctccgcctat cagcactatc ggggtgtgcc cggcattctg aaagtgcttg agcatcatca
     4681 cgcccaccag gtgacctata tgcaatgaat ctgcagtagg atcaaaacca acataagccg
     4741 aggtcttccc ctttaacagg aactcctccg tgccgggcat gatgtcatgt atcatccccc
     4801 tccacctcag ctcttcaaca aagttcatca tcagtgcttt gaattttagt gtcgcaaaga
     4861 tatcaaaacc actcagatgg tgacatcacc cttctgcttt ttgtgacggt tgaagctgtc
     4921 aatcaggtca cccccctcga tcaccgggaa aattgacggt acaagatcag caatgggatt
     4981 atagaagaaa gaattgttta ttgtagctga cggaagaaaa tgcttcttct catctatcgt
     5041 attgaggaag acaaaaatca cactgagtat gagagcggtc ttcaatacac cgaagacagc
     5101 tcccagcgtc ttgttgataa agcccaggga aatggccttc attaccgaat caagaatctt
     5161 tccaaccagg taaaccacgg taacaatcac gatgaatgtg actgcgaagg agatcactcc
     5221 tgtccaggat gagttcatgt caaaccatcc ggccagcttg tctgctgtcc accatgaaaa
     5281 gtgaatggcg ccccatatcc ccaggaccag cgccgccagc tcggctatct ctattatcag
     5341 cccgtcaatg aatccctgta tcaggccaaa agcgagcagg gcaattatta cgaaatcaat
     5401 ccagttcatc ttcagttctg tttgaaagga tgaaggtatg aaatgttgga caatcgcaca
     5461 aactgccaca aagagataaa gaagccatgc attgattgaa ccattaatta tctttgggta
     5521 ttaattctgt ttccggatgg atttgcaatt taccgatgtc attgccgtgt tcgatatcgg
     5581 cagaagctgg aaaaggttca ggctgtttga caggagtctt aacccggttc acaccgaaga
     5641 aaagatcatc gctgaagcga aggatgctaa tggttcgcct tgcgaggata ttcaggctat
     5701 cgccgcatgg atggaaacct gcctcagaaa cgtgtccgca acctcactgt acaacatcag
     5761 ggccctgaat ctggccgcag gtgacgactg cctggcgaag cttgagagtt ccctgacgca
     5821 cagccttaaa tcaggcattg ccggcacatc ccttttcaaa ggaaagggaa tgaacagcac
     5881 cgccgcatca ctgatacctt atctgaaagg atcggacaag ccctttatcc tggtctctac
     5941 aggaacatgg tgtacattca tgaatccgtt tgaccatgaa tccggtagcg tgacgcatgc
     6001 cggcaatgga aaaaccgccg gaaaacctgg cgaaaatacc ggcggcatgg aaatggatgc
     6061 tgccattacc gtcggtgaaa ggcagataaa gagctcacag ttccccctgg gtgaggttca
     6121 cgaccgcaac gtcgagatgc tggatgacca tttcggggtg accggggaac tctacaaaac
     6181 cattaagatc aagcacaaaa agatcctcaa gatgcaggcc aacaggcgcg gaagagtctt
     6241 tttccgccac ggaatacctg ccggacaggc tgacaccttg gccgatctct cccacttcct
     6301 gacttatgcc gacgcctatc accagatgat gtatgacctg actgacgagt gcctgatggc
     6361 ctacagggag atcattgctg agggtgacac aactgaaata gcctatataa caggcggttt
     6421 cgcccataat gacacattcg tgaggatcct ggctgcccgt gctgaggata agagggtcta
     6481 tgccagtaca atcgactatg ccacagccct gggcgcggct atggaagtat atcagcaggc
     6541 ttttgatgcc ggattgcctc cggtatacct gggattgaag gccattctgc tcagggatga
     6601 atagacaata ccggtcttgt ttcagggccg ccctttcaac aatgatgctt atatttatca
     6661 cttcatattc ttcatatcat taaactccct taacatgaac agaccagatt cattcatgaa
     6721 gctcatactg gtaacgcttg cagttatctt tatcacggca tgcggcaaga ccacacagga
     6781 tgacaaactc agtctcagct atgaaaaatt cataatgccg aacggcttac aggtggtgct
     6841 gcaccaggat cactctgatc cggtgatttc ctatgccatc atgtaccatg tgggctccag
     6901 cagggagacc cccggccgga cgggatttgc gcatctcttt gaacacctcc tcttcagcgg
     6961 ctcggaaaac gtccccaacg gggtatttga caaggtcatt gaaaatgccg gtggttcaaa
     7021 caacggcttt acaagccgtg atgtcactgc ctattttgaa agcttcccga aaaatgccct
     7081 tgaaaaaata ctctggctcg agtcagacag gatgggcttt ttcatcaact ccgtgacccc
     7141 gcgtaacctg gctatacagc agaatgtggt gcaaaatgaa aaaagacagg gagaggacaa
     7201 ctcgccctac ggcttcacca actacgtgat atccaaaaat ctctaccccg catctcaccc
     7261 ttacagctgg gaggttattg gcgagatgga agatcttacc aacgccaccg ttgatgatgt
     7321 caggggcttc tatgacaact tctacggacc gaacaacgca acactggtgc ttgcaggcga
     7381 cttcgaacct gattcagtca ggctgctgct cgacaagtat ttcggcgaga ttaaaccccg
     7441 cggagaggtt gcagccagga aagccatgcc cgttactctc agcgagtcaa taagactttt
     7501 tcacgaagat aattttgcca atgtgccgga gatcaatctt gtctggcctg tacctgagaa
     7561 ttataccagg gattcatatg cacttagctt cctggcagaa atccttgcca gcgggaaaaa
     7621 agccccgatg tacaaggtcc tggtgaagga gaagcagctc acatcacggg tgtcagcata
     7681 taacggcgcc gaggaactgg ccggtgaatt cgccatttca gccactgcca atgaaggcag
     7741 aaccctgaag gagatagaag atgccatttt tgaggcattt gcacggttcg agaatgaagg
     7801 gtttaccgac agggaggttg accgtatcaa ggcttccatg gaaaaaggtt tctaccaggg
     7861 gctgaccagc gtactgaaca aggcaatcca gatggcttcc tacaacactc tgctgaatga
     7921 tccgggatat gtcgggaagg atatcgaaaa catcaaggcg gttacccgtg aagacatcat
     7981 gagggtatat gaaacataca tcaagggcaa gccgcacatt gtcaccagct tcgttcccag
     8041 gggacagccg gaaatgatgg cagagaattc ggtaaaggca gtcgtaagtg aggaagatat
     8101 tacaaaggca agccaggtgg caatcaatga tatcctggag acagagattg caaagacgcc
     8161 atcagttatt gaccgcacca aggagcctcc ttcagggccg gagccggagg tgcgtgtgcc
     8221 ggaggtgtgg aaagccacat tgaagaacgg catcagggtg tacggcacgg tgaacaggga
     8281 gttgcccctg gtcagcatgt cggttgtaat agccggcgga gcgctggtag atgatattgc
     8341 gatgcccgga gtttcatcta tggttgccgc cgtgcttccg cagggaacca ggaacaagac
     8401 tcccgaggag ctggaggagg agatccagtt gctgggatcg tcagtataca tgcgcgcctc
     8461 aggtgaggaa atagtcttca gcggcagcac gctgtcacgc aacttcggca aaacagtctc
     8521 gctcatgaag gagattctcc tggagccccg ctgggatacg gctgagttca gaatggcaca
     8581 gacccgtacc aggaatgcac tgatccagtc cgaagcccgt cccaccagtg ttgcctccga
     8641 ccggttcatg aagctggttt atggccccgg taatattttc ggatatgata tcaggggcac
     8701 gaaggaatca attgaactga tcactcctga tatcctgaag gatttctatg agaggaactt
     8761 ttcaccgtcg gtcacttcgg tgtatgtggc tggaaacgtg tcaaaggatg aggtactgga
     8821 ggcactgaag ccgctggagg ctgagtggca cgacggagaa gttgcattta agagctatga
     8881 gatgcctgca gcccctgaaa agtcacagat atacttcgtt gatattccgg gctcacgcca
     8941 gtcagttgtc tacgccgggt acctggcgat gaccagggat aatcccgatt atgtaaaagc
     9001 tgactttgca aattacagga tgggaggcgc tttcaccagc atcctgaacc agatacttcg
     9061 cgagcagaag ggatttacct atggggcaca cagcagcttc cgcgagatga aaatccccgc
     9121 acctttcatc atatcaacca gcgtcagatc tgatgccacc cttgagactc tggagataat
     9181 cacaggtgag cttgggaaat accgtgaagg gataccggag gaggaacttc agttcattaa
     9241 gaacagcatg atcaggtcaa atgcactccg tttcgaaacg aatgacgcgc tggctgacat
     9301 gcttacctac atcgggaaat acggcttcag cgatgactgg gtcaaaaagg aggaagatgt
     9361 gatcaggaac atgaccgttg aagaccataa ggccatcact cagaaatact tcgatcctga
     9421 caggatgtac tacgtggttg tgggtgatgc cgccacacag atgaagcagc tcgagaaggt
     9481 aggcttcggc acacccgtgc tgctggcaag ataattgtac atgcgggggt ttccgggcag
     9541 acgccgcggg aacccccgta tcctacttaa gcttcttttt gtattcgaca agtctgagca
     9601 ggtctccgat ctgctctttt cttctcatct ccagggtgac gggatacatt gtcccgaaag
     9661 gttttgcctc cgaaagggct tccgtgatac ccgggtcaat accaagctcc ccaatgccgg
     9721 gacaatgcct ttcgacaaag taaaatccgg ctttgaagaa gccgtcccag acagatagcc
     9781 agaacaccgt cttctttttg aagaccacct tgcagagcca ggctttgccg tcattataga
     9841 atctccactc aggggtcagt ccgtactctg cagcagtgat tgccttcatc atttcatcgt
     9901 atgcgtggta gctcgcgcca aggaggctct tcaggagctc ctctgacgga ggaattccgg
     9961 gatcattcag taatggctta ttcatttgaa tactgattta agtgtttctc atcagtccgc
    10021 ctgcaacgca cctccttatg tcccggcaag cgcagggact aaacctcaag acccagcaag
    10081 gccagctccc cgcgcagctg gtcaggcgag ctgaaatgaa ttgcattaaa tccaagctcc
    10141 gtggcagcga tggcgttcgg tttgttgtca tcaatgtaca cagattctgc cggattgatg
    10201 ctgtacctgt ccatcagaac gcggtagatg gcatgatcag gcttgagcag cttctcgtat
    10261 ccggaaacca caagtccgtc aaactccttc atgaagccgt aacggttgta agccacagga
    10321 aaggcctcat gtgaccagtt ggaaagcgcg tagaggggca ttccggccga tttaagcctg
    10381 cggaatatct ccaccgtccc aattatctct ccgtcaagca tctcctccca gcggccgtaa
    10441 taaagggcaa ttttgtcact gtactccggg aatttctgct gcagctcggc ggtggcaaca
    10501 ctgaagagct tgcctccgtc ctgctgtaca ttccattcat aggtgcagac attggccagg
    10561 aaccattcca tttcctcttc gttctcaaat acttttctga agaggtgcct cgggttccag
    10621 tcaatcagga cccctccgat atcaaatacc acgctggtga tttttttcat cttgctttaa
    10681 ttccggcgca atattagtca aatttcgcag ggtgtaaaag cccaagcctg aagagcccta
    10741 tcttaccctc accgttgctg tctcattttt cagtgtgcct gaagcgctcc ggtactccgc
    10801 aataatggta atttcgccgc tcttttttcc tgatggaata tccagatggg ccttgcccga
    10861 aacatccgtc cttacttcca tgccacgcag gatcactttt cctgaccggg cggaggctat
    10921 gctgtcagca gcattccccg gaatctctgc tccgtcactg tttatttccg tgcctgattc
    10981 cccggtttta actgtacgtc ccgagcggat tcttccagtc tttgtccata tttctatcac
    11041 tccggtggtg ttgaatcctg tatatctttg aatatccaca aattcagttg agacatttat
    11101 cctgtcgata tctgacggat tcatgttttc aatcaccctt ggatcctgac ccaacatgat
    11161 cccgtcgacc actatgagtg cgccatcctg tctgccgttt gtatcgggtg ccacgcttgt
    11221 aaagattatc cggccgtctg tcagtctgaa aggcttgatc tgctggatca tactgaagac
    11281 atcattatgt gagacatttg ttccgggata accacctgag cctcctgacg gtcctttatg
    11341 taaactgcgc cttgaatgca cggcatatat ggtaatcaaa gcctgcaccg gctcaccatt
    11401 tacatctgca gcttttacgg ttactgttat agtatcttta cgaccaacat ccggtctgtc
    11461 agcagtcaac ccgatatttt gtccggggag agacactgaa gtcagtgaca tgacagccac
    11521 tacaagcata attaattttc tctgagccat ttgtaatatt cctccatcaa taatagttta
    11581 tccatgtttg gtacgcctat tggcaaaggt acggagcctt tgaggtacat tacggtttct
    11641 ttaccttcct caaatacagg ccatcccgga agaccctcgc cattcgggtc accattcttc
    11701 gcaaagttag tccagtattt tatcattcta tcacttagaa taatctcttc ctcggtggcc
    11761 tttgcatcag gattctgatt cagatgcccg aagacgtacg gtatctcgga ggcatgggca
    11821 gcgccccttt gcggggaccc tcccgggaag agaggctcct tccggaactg atcaaaatag
    11881 taaacataaa cccttgattt acctgtcttt gactggagcc ttgcccatgt ccacgagggc
    11941 catccgaaaa ccgcgtcacg gaagatatct gacatggagg catacgtttc atattcggta
    12001 ttgcccggat aaagctgaag cgccttttcg gccagggggc cgaacctcat tttcaacacc
    12061 ccggcgtatt cctcaggttt cacaggctgc gtaaacattg aaccctcatc cgagttggtc
    12121 ccgataataa cattcacgtc gttatagttg cctgcttcat aaagcttgta ctggtcgtca
    12181 gtgatgacat agccatccac gacaggccag aagcctccca tctgcgaccg gggatcggaa
    12241 agccatttat caggagacat ggctcttagt tcatcaatgc tgtttgcacc cataatcttt
    12301 gcaaactcca taccggcaag ttcggcgccg gcaagtgtct gcatgaaatc acctgatccg
    12361 ctgttgtctc ctaccgggcc gaatgaaccg ccgctctggc ttatggcccc ggcgaaaagc
    12421 ccctttgtca gcggtgaggc acagagcatg cttacggaga ttgctcctgc ggattcgccg
    12481 aagactgtca cccgggccgg atcacccccg aaggcactga tattctcctg tacccatttc
    12541 agccctgcaa tctgatcgag caggccataa ttccccgata ctttattttc tgactcagca
    12601 gtcaggtcgg ggtgagccat aaaacccagc acacctacac gataagctat gctcacgagt
    12661 atgacgccgt gccttgcaat ctggtctccc caggtgtcgg caccggccgt tcctccgaag
    12721 gcaaaaccac cgccatagat ccataccatg accggcagct tctcttttgc cgactttgca
    12781 ggagtccata cattcaggta aaggcaatcc tccgaagttg gcctccctcc catccatgct
    12841 acggcaggct ggatgcttgc cggtgcgaat ttgtcggctg tcagtacacc atcccatggc
    12901 ctgactggct gaggtgcctt ccagcgaagg tcgcctaccg gaggagcagc gaacggaacc
    12961 cccctgaaga cggcaatgcc atcctccaca gttccctgca caacaccacc cttaaccgtt
    13021 gctgtggcgt ctgaaagccg gtctccgccg catcctgcaa aaagcattat caccgagatt
    13081 actattagat taaccgtagt tttcattgtg ttgttcatta gttcagttta atcgttgcga
    13141 ctccctttag cgcctctgcc gcatcagccg gcagtttcca gttgttgtca aaagagccct
    13201 gggtaattat gtagtctggc gtttctttga agagaatgaa atcctggtac tttttaagga
    13261 tttccacgtt tgttccgctg aaggcaagtc ctgcctgggc cgtcttgccc ttcggaggcg
    13321 tccaccaggg caaacccgaa ttgacgagta tgtaatgcct gttcatgggg aagatataga
    13381 caagtccgta attctcagcc ttcggatcaa gatgcatcgg gagcttgtct gcaaacttct
    13441 ctataactga atttgtttcg cgtgttccga agaggatgag atttgaactg acgaagtcac
    13501 ttgaccgcac atccctgtca gccaccaccc tgggaaatac cattatcctg cctcccatgc
    13561 cggtccagtc ggcggcggcc atggcctgcg cgcgccttgc ggccagcacg tcagccggag
    13621 ggttgtcggc ggtgccgtaa acatagatgt ggcttccggc cactgcagca tacagaggcc
    13681 cttcggcacc cttctgcttt gaggtaagcc caggggtaaa cctggtattt acccagtttc
    13741 cttccgaaag caagaaagac actgcatcgg cgctcctgat gctgaaagtt ttcccgttta
    13801 cagttactga cacctttttt gaggggctga attgcgggtg accatcaaga cggagtgtaa
    13861 aggcatttat tcctgtgact gtgactgcaa cggagttagg gccggtaaac tttgcctctg
    13921 cagtcgcagt ttttccgggt atcatgtcat caattgtaac ccagtaggca ctgttgtatt
    13981 tgaaccattt tgtcgtgaat gagaccttcc cggggtacag atccctctta aactgcgaga
    14041 accagtcaaa tatgaaacca tccttgtagg cccactccca gctgttgtgt ccaacgccag
    14101 ggtactccac gtaatcaagc ctcatggctg ttaccccaag cttaaccttc cattcagtgg
    14161 cggtctggaa gagaaaatcc ttgtcgccta tgaaaagatg taccggcagg ttggaggcat
    14221 tgccggcaag ctccatggtt ccttcaggcg gtgccgggca gacaggagct attgctgccc
    14281 atacgtccgg cctggtaagg ccaagccaga gagtaccgcc gccacccatg gagagcccgg
    14341 tgagatatac cctgtcttcg tcaatatgaa accgcgcctt gagatcatca atcatatcat
    14401 agacatcctg ctccggtatg ccctggtaac cggcggtacc tcgtgccagc ggcgctgcag
    14461 ctatgtaatc aacaggcctg aacggcggcc agtagcggga agcttccacg tcgttctcga
    14521 aggcgacgtt gcccggtttg atgaaatcat atccctgggc gttgcctacg ccgaaaagac
    14581 gcctcatccc aagcctgtga ttagaccagg caccatgcag aaacaccaca agcggatagg
    14641 atttcgattc gtcaaaattt tccgggatat agaccgagta tggctggtca gtgtcatcaa
    14701 ccgatgagaa aaatgaaagg tcctgcggcc ctgatttcag tcgctgcgct ccggcctggc
    14761 ctgaaaagca ggtaaagaca acagacatca ggataaataa acgcattgtt gaggtaatga
    14821 tattcatttt attgtatttt atatttttat gatgctatct gattctttct tgagataaaa
    14881 tcagggtgac ttgtctaaag taaactttta atttatataa caggcgtaac tccctttata
    14941 agaataatat taccgtattt taccgtttcg ccggtcatta ccacggcaaa tgcctttgag
    15001 gcgcgctcgt agaaggcaaa gcggtcaatt tttttaacgg gtggcactcc cggagctgac
    15061 ttccttatct gttccagata cgagtcttcg acaaacgggt cagcctgatc gcctggcacc
    15121 ggctccatca tcattaccgg atgagggaca taactgtcga gttcaaacag cggcatgatt
    15181 gcctccagca aatcggcaat gcgcaggccg tctgccctta ccaccctctt cccgatgctc
    15241 tcaccgggaa aatgtgcatc agccagaacc agctcgtcgc cgtggcccat gcgggccagc
    15301 acatccagca ggtcggggga aatgagggga gagatacctt taagcatagc tactgttgtt
    15361 tgaccggtga atttacaatt ttcggaggac cggggaaaat agcctgcctc taaatgcaca
    15421 ttaatatccg gtttttaata aattagcaga cctgagactg aaatatttaa atataagaga
    15481 gcaaattaca tcctgcaacg aaaaaaatct gcatcatgaa caatgaccgt cgtgaatttc
    15541 tgaagaaagg cttgctgggc gtttccgccg cagcatttat tcccggtgtg atcaagggct
    15601 ctccggctgc agccgaagca tcagcctcac tgcctgatct gccctcaaga gtcctgggcc
    15661 gtaccggaat acaggctccc cttatcagtc tcggtgccag tggcgcgatt actcccagct
    15721 tcatcaaagg ggcatataat gccggtatta aactcttctt ctcagccacg tattacggcg
    15781 aggggaataa tgaaatcatc gtcggtgaag cccttaaggg ttttcccagg gactctttcg
    15841 tcgtagccac cgcagctccc tgcaacgagg ttgataaccg aaccggcctg ctgactgaca
    15901 agttcagcgc cagggcatat atggagaagg cggaaggcag cctcaggcgt ttcgggcttg
    15961 accatatcga tatcatactt tttccatatg catccaaaag ggagacggta tgcaacgacg
    16021 agatcctgaa gacaatggaa caactcaaac agcagggtaa agtcaggttc gccgggatag
    16081 cctcacacgg cggcatcccg gaagcgctcg acgcagctgc atcaaccggt atctatgata
    16141 tagcgatgat ctcctacaac ttcaagtcag agaacagaga ggctctcgat accgctatcg
    16201 cgaaggcttc agcttcaggg atgggcattg ctgcaatgaa gactaccgcc ggtgcattcc
    16261 ggaacaagag cgggcccggc ataaactctg acgcagcatt gaaatgggct ttgcagaatg
    16321 tcaatatctc cacaatcgtt tccggcatga caactgcaga acagattcaa aagaacctgg
    16381 caatgatcaa aaatctgaag atgaccgaag aggaaatggc ggatctgaat accgcacttg
    16441 cattttcaga aacaggattg ttctgtcagc agtgtggcca ctgtctgccc cagtgccccg
    16501 ccagccttga catacccacg ataatgcggg gttatatgta tgcttacgga tacaggaacc
    16561 tgccccaggc aaggaataca ctggcagcat ccgggctgaa gtccgggcca tgtgctgact
    16621 gtaactcatg cagcgtaaag tgcagctcgg gttttgacgt cagggggaag atacaggaca
    16681 tagcgcgcct gataaaagta ccagaagact ttttgctgac ctgatgtaat tgaaaacacc
    16741 ggtactcttt gttcctgcca tgacggtaag gctgccggga acaatagagc cagtggtctg
    16801 tttataccag taagaacatt attaaatcat atgaaaccaa cagatttttt cacgctggct
    16861 aacggcgttg ccatcccggc aatcggattc ggaacctggc aaattccttc aggtgatatt
    16921 gcctacaact cagtaaaaga agcccttaag gcaggctacc gacatattga cacagcgcag
    16981 gcatacggca acgaggccag cgtgggaaaa gcaatactcg actcgggcat cccccgccgg
    17041 gagatattcg taacatcaaa actgccggct gagataaaaa gctatgacaa ggctgtcctg
    17101 catgccgaac tgacgatgaa aaacatcggg cttgactaca ttgaccttta cctgatccat
    17161 gccccgtggc cctggacaga gatcggcgcc aactatacaa aagaaaacat cgacgtctgg
    17221 aaggcaatgg aagagttcta tgaaagcaga aagacacgct ctataggcat ctccaacttc
    17281 aatgtgaagg acacggaagc aatcctcgga acctgtcgca tccggccaat ggtcaaccag
    17341 ataaaactgc acatcggcca cagccaggaa gagatcacgg aattctgtcg caagaacaat
    17401 atcctcatcg aaggctattc accgctggcg acgggcaaaa tcctcaacaa caaagaaata
    17461 gcccgcatcg cggaaaaata cggcaggacc cccgcccagg tctcagtcag gtacgtcctt
    17521 cagaaagggg cgctcccgct gcccaaatca gttcatcccg gttacatccg ccagaacttc
    17581 gatgtaaact ttgaaatatc caatgatgac attcgccttc ttgacagtct gaaataatat
    17641 ttatattgtc cggcaactat tgaacaattt ccgataatgt tgcctgaggc gaggtattac
    17701 aagcccgcgg agaagctttc gtggctgata aaacagtttg aattcattac gtacaacggt
    17761 aaagaggctc ttacagacaa gtttgtgccc cgtgaggatg tctccattgt ttttcatttc
    17821 cggaaccggc cacggatggt ttcacctgtt gaccagacac ttcctccggt tttcatagca
    17881 cccctgacac ctacggccaa ctcaatctgc ctgccgggag ggaatgacag catgatcgtc
    17941 acctgcaggc cgacagtatt gtcacgcatg tttggcatca gcctatcgcc cggcaataaa
    18001 atctgggtgc cagtaccgga agggcttctt ttgcctctct gggaagagat gaaacagggc
    18061 aagtcagacg ctgcaaggat ggatgtcttt gcctccttca tcagcagcaa ctggcaccgc
    18121 cggtacatcc ctgacgagac tgacagaagt tatgacctga tactgaggaa agggataaac
    18181 accaaccttc atgacatcac tgacgagctt caggtcagtg aacggacact ccagaggaga
    18241 ttccgtgacc ggctcggggc aacgcctaaa atgctgattc gccttatccg gatcaattat
    18301 ctgtgggaaa caacctcggg aggcggaaaa atcgattacc aggacctggt tttcctggga
    18361 agttatttcg atcagaccca tatgatcaag gatttcaagt caataaccgg ggaaacaccc
    18421 aaggcttttt tcaggagaaa cctcagcatg gcgaggatct tcagcggaaa gtgaccttcg
    18481 cagctctgca ttgcatgtcg gttttttact atcccgcctc tgaaataatt ccctgtttta
    18541 aggttaaaat tttcactaaa accggaatta tgaaaatcag agtcttcttc tcaatagctg
    18601 tgatattgtc atttttactg tcagctcaga gcgtcaatgc ccagatagtc aacccaaaag
    18661 atgttgttaa ggaaaagagt accaacagaa taaacaccag gatagaccag ggcgttgacg
    18721 aggggttcaa caagattgag gagggtgtcg ggagcctgtt taagaaaaag aaggtaaaaa
    18781 gcggcgaaga ggcagaggca gaggaaaatg agccggatac tgacaccgaa gcagaagaac
    18841 ctgaggctga aaggcagaag gatgctgcac ctcagaaaca gaaactcgag agctttacac
    18901 agtatgactt tgtacccggc gacaggatac tttacttcga ggacttcagc caggacgcca
    18961 tcggcgattt cccggcgcta tggacctcca acggcagtgg agaggtaaaa tcagtaaata
    19021 tcgctccggg aaaatggctc catatgaacg gtgaagatgc agtatactgt tactcaaaga
    19081 ctgttgcgtt tcccgacaac ttcatagttg agttcgacat aatccctgat gaagaatacg
    19141 gccgcggtat tgtatttaca atgtatgaag aaaacccgga agaccccaag gagatcaatg
    19201 acgatcttta ccccggtaaa agcggattcc acgttatcct tgaaagtggg ggatgggaaa
    19261 ccaagggtta taccaatgac ccggaaggag actggataga gggacaggca tcaaggaatc
    19321 cggtgactac cgaagaagtc aaccatgtta tcatctggat acagaaaaga agggccagga
    19381 tctatcatca gggagcaaag gtgcttgacg taccgacaaa catatatgct cacacaaaat
    19441 tcaataagat cctgttctcg ggctgggaca gggcatcctg gccaatggtc accaacatca
    19501 ggataacctc ggctgccccg gatacccgca gcaagctgat caccgagggg aggctgacaa
    19561 cctacggcat caccttcgac gtcaacaagg ctgacatcag gcctgaatca cgcggaactc
    19621 tcaatgacat agccaccgta cttaaagaga atccggatgt gaaggtaaag atcatcggtc
    19681 atacagacag tgacggtgat gatgcgctga acctcgacct ttccaggcgc cgggcagagt
    19741 cagtcagaac agaactctcc tcaaaattcg ggatagatgc ttcccggatg cagacagagg
    19801 gcgccggtga aaccaaaccg gtggctccga atgacacccc tgccaacaaa gccatgaacc
    19861 gcagggttga attcattaaa cagtagattt tgccagtaac ttttcttcat acagatcctt
    19921 ttaaccaaaa accagactaa aaatgatgat aaaaagaatt attgcctttg cggtgctgct
    19981 gcttacggct ggcacaatga ctgcccaaaa ttgtgttttc tatttcccgt cagaggttgg
    20041 caccagagta gggtacaact actattcaaa gcccggcaaa cttgaaggaa gctcaacgct
    20101 gcttgtcacg gacaggagta cggaaaacgg caacctgaaa cttgatataa aagccactgt
    20161 ctttgatact catgaagaca cgacaattac atttgactat gcagtctggt gtgacggcga
    20221 caacttttac attgacatga gaagcttcct gggttcaatg aacattaccg aactgggtga
    20281 gttcaaaatc acatctgctg atatggtgct tccggcaggg atgagcccgg gacagcagtt
    20341 gaaggatgct tcattcacac ttgaaatgac cagccctatt ccggtgacca tgacctccaa
    20401 tataaccaac aggaaggttg atgcaataga gcaggttacc accccggcag gtacatttga
    20461 atgcgtcaaa gtctcctacg atacattttc aaaggtagcc ttcatcaaaa cagaaggccg
    20521 tacggtggag tggtacaccc caaacatcgg catcgtacgc tctgagtatt atgacaagaa
    20581 aggcagggtc acttctattc atgagctttc gtatgtgaag aaatagttgc cgggttggag
    20641 gcggttttta tcatcataat aactaacttt aaagcagtct aataactgct tcggtgatca
    20701 ctcctgcccg caagaggacc ctttcgctgc tgaccaatcc gttcttcctt gcagcgatac
    20761 cttccattgc actttttttc atcatccctg tcatccacca gcgctacagg cttgacctga
    20821 agtcgtcaac actggtaccg gaagggtatt atcacagcta ctgcgacctt gacggtgacg
    20881 gcaactctga aaagattgtt gcgattgaag aggagaatgc atctggcata acaatttcca
    20941 gggataacag ggtactgaac cagtggaacg taagaggaag cttcagtttc tccaacagga
    21001 acactattta tattccggct gactgcaata ccgacggatt gaaggagatc tatctcttct
    21061 ccctgtccgg cgactcagtg cttcttaact gcattcccgg acccgaaaca aataagtttt
    21121 ccgttgccaa catgcttgtt gatgttgtcg ggcacggtat caaagccccg gatccgtcaa
    21181 tcgttgctgc agaaccggag gaccttgacg gtgacgggta ccccgagctg atattcggca
    21241 tttccaccgg gttctcaaaa catccccgga aggtatatgc ctacaataca gtgacagaaa
    21301 caataatctc ttctccccaa tgcggaggtt tcatgctggg tatagtccag catgacctga
    21361 acaacgacgg tttcatggag ataatgcctt acggttacgc ctccgaaaac attggcaggg
    21421 atgaaatgaa atatcatgac tattctgcgt ggcttacggt actcgaccgc aatctgcagt
    21481 ttgtctttga tcctgtggag ttcggcggca gatataccat ggcattacca atgatccacc
    21541 ccctgtcagt ggatacggtg gccggtctgc ttgtcagccg gggggaacct gattcaacat
    21601 ccgttttccg gtatttcagc atgcatggtt tactgccggg caggacaccg gtcaggtcat
    21661 cagcccataa cggaatgatt accgtcaaca ggaaaggcga acccctgtat gcacttgttg
    21721 actacgacag gggaattttc atccttgacg aacagaaaca gctgaaaaag acaatggaat
    21781 tcaccggcct gcgtcaacta aaaaccatgg accttgaccg tgacggctcg gaagaaatcc
    21841 ttgttgtcag cctcggagag agaatggcga cagtattcag gtcaggattt atccggcctg
    21901 ccatggcaga cctgggcaat gaactaaggg gagaatctgt ggcatcagtc atctgcagga
    21961 aaggtgaatt tccgcaggtg ttcctgcagt cagacacgag gcagtttgtg ctggagtacc
    22021 gtcccaatcc tttctatttt gcatccttcg tattctaccc tctggtttat gccgctttca
    22081 tagcctttgt gctactgata cagttcagcc agagaaagct tattgccagg cgtgaggatg
    22141 aaaaaaagaa gatcacagag ctgcaaatgg cactactgcg aaatcagcta gatccgcatt
    22201 tcacgctgaa tgtcctcagc tccgtcctga gcatggtgga actgtcagaa aaggagaaag
    22261 caagagacag cctgctccgt ttctcggggc tctaccgtga actgttgctc tctgcaggca
    22321 agtcaaggcg cacactggcc gaggaacttg agttctgcag gggatacctg gccctcgaga
    22381 agatgaggta ccttaaccga ttcgattata tggttgatgt gcagcctggc atcaatgaag
    22441 agctgcccgt tcccaagctt gttattcagc tctttgctga aaactcagta aagcatggcc
    22501 tggcagggct tgatagcggc ggcctgctcg gaataaatgt aactggaacc ggtaacgagc
    22561 tgactattga ggtgaaagac aacggtgtgg gccgggcaca tgcggttaat gatcacagca
    22621 gctccaccgg cagagggatt aaattaatgt ccgacctctt cgatatgtgc aacggctatt
    22681 tcgaggaagc acacagtttt acagtctctg acctgtcaga cagtgaggga aagccttccg
    22741 gaacactggt tacaatcaat atcatctatc gtcaccagtc tgtcttcctc agctgacaag
    22801 gctataaaag cagagcggga gtgtcgctcc cgcgctcact cccgctctgt ctcagtagcc
    22861 ccaccaggaa tcgaacctgg atttcaggtt taggaaaccc gcgttctatc cattgaacta
    22921 tagggccgcc tctttgcagg cgcaaatgta aattattttt cggttatcgc aaaaaatcag
    22981 cacccgcccg aaggcttctt cttcgccttt gacgctacgg gaggcttgac agcctttaca
    23041 gccgttgcgc ctccctcggt ggcaacaatg ttcccgccgc ccagggcggc agcagcgctc
    23101 ttgcggaagt cgtacttcgc aaactcttca ttggggcttg tctgggaggg cgcctgcggc
    23161 gaaagaatgt tcccgaacca gtagttcagt ccaccctcga gcacgaagtt gttctcataa
    23221 ccgagctgcc tggtgatcat ccatgcctgg ttcgcgtaaa cagagccatt ggaatataag
    23281 acgttcatat aggcatcctg gtggaggata tcagtgtact catcggaaag aaggctgctg
    23341 agcgggatgt tgattgcccc ctccatatgg taagcatcaa actcctccgg tgtgcgcacg
    23401 tcaatgagcc tgagtgacgg gtctttctgc accagcatgt cagccaccac gtcgggtgaa
    23461 acaaactgga ctccggcccc ggcctcctgc agcaactcgt cggctgtcag ccggaagtgc
    23521 ttcactctgt cgggcggaac ggctgcaatg attatcccca gcgggatcac aaaaagcgca
    23581 agtaatctaa gcggtttcat cctgttgatt tttttgttgt tacaatataa ccaaaatatc
    23641 cggcgcaggg tttattctcc acgcccccgg caatcagtac tcctccctcg ggaatttcct
    23701 ttcagcccac tcggccacag cgaacatgcc aatagcagca ataataagca gcagcccgaa
    23761 gagcccgtca gacatgccaa gtttttcact cacctttggt tcgccaaggt atctggccat
    23821 atacatcttc tcccaaaggg gatatgtgaa tgcaaatccg taaaccccaa tgaagagtcc
    23881 tgcaacaaac accatcccgt caatcttgcc tatagcagcg ccgcagaagc tggtgcccgg
    23941 gcagaacccg cccatgatga agcctgcacc catcaccact ccgccaataa tggcagaagt
    24001 gagatacgtc gggttaacat atatcagatc gaggtcaacc catccgaaca ggctcatgaa
    24061 gagcaatcca agcatgcatg ttatggcccc ggtgaagaag accttcagaa cgacggtgtc
    24121 atagccgtag aagatgccgg cgagcttgcg gctcgatgaa aagccggagc tctcgagcac
    24181 ccatccgaat gctatgccta taaagaatgc caggagaaag ttggtgttct ccgatattat
    24241 tccatttggt gccagtggtc ccattgattt cctcctttca ttaaatccag tttctcctga
    24301 agaaatatgc cagagcgaag gctgttccga agatagccat cattgttacg aatcctccca
    24361 gcgagagaac gcccattccg ctgagggcag agccgctggt gcaaccccgg ccaagctgcg
    24421 agccaaaacc gaacagtata cctcctgcca gtgcgaatat cagtctgcgg cgggaagtga
    24481 ttttcggcga atgttccact ttcagcttca gccttccggc cacagcgccg gagaggaatc
    24541 ctccgacaat gacacccagc atctggaaaa ccagccatga cttcatcggg ctgccgctgt
    24601 gtgatccggc atactccttc ataaagggca ttgtttccga tgcattcctg tcaactgcct
    24661 caatggtcgt tgcaacgacg cttttaacgg cgccgctggc ccccagaccc ctgcccgaga
    24721 caaagttggc ggcaagcagt acgaggccaa gcagcacccc gcccaggtaa gggttcaggt
    24781 acctcctgct cctctcattt gttgtattgt tgctcatttt atgttgaatt ttattgtgat
    24841 cagtataacc agcggctcac ctggccggcg aagacaaata tgaacctgaa cataaaactt
    24901 cccagcagta ccagtacgcc cggaatcacc gcagggatgt tttttccgcg gagttccatc
    24961 aggtcaagga caccgggaat gattatcccc agtatcacca caaagatcca gaataccatc
    25021 gtataaggcc cgccgaggaa gagctcagct gcctctatct gaacctgggt gcttgccagg
    25081 aaacccatga acatgtgaac tatcaggaaa agctccaccc cgagtattat caggtctatc
    25141 tttgtgaaca gcagcctttc ttccctgttt ttggacatca gtactattgc tgctgcaccg
    25201 gccgaaagtc cagatgccag gaatagaggt ccgagcaccg aggtgttcca cagcggcctg
    25261 gcattgaatg ccgaaagcag tatcccggta tacactccgg tgatgattgc aaagatcagt
    25321 atcacccagg caagctgctt cttatacttg ttgaagaaga cttccaggtc cttgagccag
    25381 ttgtacttcc agtcccatcc agggaaaaac tccctgatgt gaagcgcgca ccagatgaat
    25441 gatgcggtgg ttatcaccag cagtgtccag gcgccccatg acatgggaga ttcaagcctt
    25501 attgtggtat agagctgcca gaagaatgcc ttgtgacgca ggtcatagaa gagtgcggcc
    25561 aggccaagca ccagggctat cggggcaagg aagggcaccc ggcgcactgc cgagcggtat
    25621 tcgttctcct tgcccatcag gtagtagaga gccgcaaaga agagcacacc ggcagcaagc
    25681 cctccgagaa aaaggtacag aggtatcggc cagtgccaga tatgcagttg agggtcaacc
    25741 atcaggttct cccttccgct tatgataagt tcttcgttca tggtccggtc agattaggaa
    25801 atagagatgt ggtttcgttc ctgcctcggg tatgagggtc ttgtacttgc gctttttaag
    25861 caccatactc acatcgctgt ttggatcatc gaggtcaccg aagtacatgc acttggtcgg
    25921 gcataccgac acgcatgccg gtttcatccc ctggctgacc ctgtgaacgc agaaggtgca
    25981 tttgtcaaca taaccctttg gatgagggta acgggcgtca tagggacatg aagcaataca
    26041 cgctccgcac ccaatgcatt tgttcggttt gaccagtacg atccccccgt cgactatatg
    26101 acttgcaccc gtggggcagc acctcacaca tggggcattc tcgcagtgat tgcacctctc
    26161 tgaacgaagc tcaatctcca gctgcgggta ggtgccgtcc atcacttcca ccacccagtc
    26221 acggcagtat ccgatcggca cattgttctc ggtctggcat gcaacgacac agtcactgca
    26281 tccgacacac tttttggtat cgacagccat tgcgtatctc atgacaccac ctcctctcct
    26341 gccaggtcag taataaaggt cacgaagttg ccgcgcatgc ctgtacctcc cattacggga
    26401 tcgacaagca cctttgtgat gaggtttgtg tcactggccc cctttccgta agaccgtcgc
    26461 agctttttct cctggtgccc gaatccgtga accatgtata ccgagtccca ccggatccgt
    26521 tcggtcaccc tgaccttaat cgggaactca gacctctttc cgtcctggtt taccaggtga
    26581 acatactgtc cggtgctcag tccccactct tttgcaacac gcggattaac ccatacgctg
    26641 ttttcatcca tcaggtcagt caggttcggg ttatttgcag tgcggctgaa ggtatgcata
    26701 ggggccctgc cgtaattgag ccggtaatat ccggccggcg gctcctcatg agctgtatac
    26761 tttggcatcg ggtcgaaccc ggctccttcc agcgcggttg agtagagctc tattttcccg
    26821 gtggcagtat agaattccac gtcctcatca ggatagaaga agaggttatc agcagtgcgg
    26881 tcaaaggttt tgactccgat cttcttcatc tcctcaagac tgcttcccac ctgtttcagc
    26941 tgccagtcaa gctgatcttc catcttctca aacgggaaat attcaatcac ccccagtctt
    27001 tttgcaagct ccctggccat ataaaatgcc ggctttgtgt cgtgcatggg tttggcagca
    27061 ggtgccctga gggcgatctg cggtttgcgg tgcggtccga ccctcaggtc atcatatctc
    27121 tcgaggtagg tgcactcggg aagaacaaca tcagcccagc ctgttatttc catgggcatt
    27181 gtatctatca ccaccagcag ctgcagctcc tggatggcct tgagggtatt ggcctggttg
    27241 ggaagggtct ccatcaggtt tgtgccgttg actatccatc cctggaattt gcagttgcgg
    27301 ctcggatcag gcaccgtggc gtcgcatacg ccgttggcaa gtaccgtgtc agcaagcttg
    27361 tacagatcgg gaaactcagt cctccaggtt ctctttgggg tgggataggc aggatgaggc
    27421 ggctcgggca gctcgaaggt ttcggtgcgg aagaagccgc ccctccgccc ccatgagccc
    27481 agcagggcat tgagaattgc cacagccctc agtcgctgcg tgtcatcacc gtaccaggtg
    27541 acatgacggc ccgggtggac tatcactgca ggtgaggcgt tggccatttc acgggcagtc
    27601 tcacgtatga ccgtcggatc aattgttgtt atgccgtaag cccattcagg ggtcatgttc
    27661 ttaacatgct ccttaaggta ttcaaaaccg taggtatatt tctcaacata aggcttgtca
    27721 tacagctcct cagtgatgat tacgttaatc catgccagca gaagggcaag gtcggttgcc
    27781 ggttttatcg gcagccagta ttttgacttg ccggcagcag tggagaaccg ggggtcaacg
    27841 gttatgattg ttgctccctt gtctatggcg tcagacatct cctggacctg tccgttatgc
    27901 atattctcgc cgagatgact tccgatcagg acaagacacc tggtatcacg gatatcaaga
    27961 ggctcggggc tgtcaagcgc ttctccgaag gttgtctgga aggccacttc gcgcggaccg
    28021 cggcactgtg cgtaggatgg cgctgcaata tgtggcgaac cgagggcacg aagaagggtt
    28081 gagaaatatt tccctcccga accgtgggtg aagagtgcga tgcattccgg tccatgctct
    28141 gcagagatca cctttatctt ttcggctatg tagtcaaacg cctcttccca tgtagcttcg
    28201 cggaagctct gcatgccgcg ctcttctgtg cgtatcaggg gtttgcgcag acggtcctcg
    28261 tcgtggtaca tgcccactcc gccggtgccg cgcggacaga gacggccgtt gcagttgagg
    28321 tcttcactgt tcccgataat cttctgaatt gatcccttat cgtccctgta tacccatcct
    28381 gcacacttcc agaaacatac ctcgcagtat gtgggggtgc ggccggtcag ggcaacatag
    28441 gaggccttcc ggccgtcagt gatctccgag gcaaggagcc gcagtccggc ggcaccggcc
    28501 atgacagctc ccgctcccat cgctgagatc ctgatgaatt gcctgcgtgt tgccatgcta
    28561 tagtattgat ttgctgtttg ttcaggttcg gatcagcagc agagacgtga attccgtaac
    28621 tgctcatacg ctgaccaacg aaattactgt tttaaaacta acagcaattc taaaatgatt
    28681 tttatgatac ctgcgaacag gaaaagtgtt caggaaagct tcttatacct gaatcttacc
    28741 ggaccggcat taccggttcg tctcctgagg ttttcatcat actccgagaa actgccctca
    28801 aaccagacca cttctgaatc accttcgaag gcgaggatat gtgttgcaac acggtcaagg
    28861 aaccagcggt catgggagat tataacggcg cagccggcaa aattatcaag accctcttcc
    28921 agtgccctga gtgtgttcac gtcaatgtca ttggtaggct cgtcaaggag cagcacgttg
    28981 gcaccgctct tcagcgtcag cgcgaggtgg agcctgttgc gctctccgcc ggagagaacg
    29041 ccgcacttct tctcctggtc ggcaccggtg aagttgaacc gggcagcata ggctcttgaa
    29101 ttaacaggcc tgttgccaac cagaatctca tcctggccgc cggagataac ttcataaacg
    29161 ctctttttcg gatcaatggc ggcatgagcc tggtcaacat aacccagggt aaccgttcct
    29221 ccaacctcaa atgtgcccct gtcagtggtc tcctgaccca tgatcatccg gaaaagagtg
    29281 gtcttgcctg cgccattggg gccgattact cctacgattc cgttgggtgg aaggctgaaa
    29341 tcaaggtctt caaacaggat tttctcaccg aaggccttgg tgacgcctcg tgctgttatc
    29401 accttgtcac caagtctcgg accattgggg atgaatatct cgagcttctc ctccttctgt
    29461 ttcacatcct ggttaaggag gttctcatat gcactcagac gggctttgga cttggcgtgc
    29521 cttgccttgg gtgacatgcg cacccactca agctcccgct ccagtgtctt gcgcctcttg
    29581 gtactgccct tctcctccag ctccagccgt tttgccttct gctcaagcca tgaggtgtaa
    29641 ttgcctttcc atggaattcc ttcgccgc
//
LOCUS       DAOPXU010000027        29177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_1590_length_29177_cov_10.996188, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000027 DAOPXU010000000
VERSION     DAOPXU010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 29177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29177
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1590_length_29177_cov_10.996188"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PKK03_04320"
     CDS             <1..209
                     /locus_tag="PKK03_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX83652.1"
                     /translation="AKNAGKHIPSLKDAFFVDDEAAHRGYVTYSEPNAGKTTGFQAGK
                     HEWFPFPYSVTSINPNLQQNPGWE"
     gene            complement(530..1195)
                     /locus_tag="PKK03_04325"
     CDS             complement(530..1195)
                     /locus_tag="PKK03_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83653.1"
                     /translation="MKNKARVLYIFLALLFVAEGAYAQRDSLQNLPNLLLPKFITSVV
                     KMKSGEKRRAVLNYNIVDQEMVFMQRDQFFVLNDPQLIDTVYMANRTFIPFEKGFYEL
                     ALRGPVTLFIQHKAYVESAGVPTGYGAMSQTTSYNYVKAVYDNNGAINLKLPDEFKIT
                     DDTEYWVLKDGEMKNFDNKRQFLQIFPERGKELKQYLAKTRLDFDDYKAVIKLVEYCN
                     TIL"
     gene            1437..2648
                     /locus_tag="PKK03_04330"
     CDS             1437..2648
                     /locus_tag="PKK03_04330"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HNX83654.1"
                     /translation="METIRNLYRIGRGPSASHTIGPARAAQSFMAQNEGAAAYRVSLY
                     GSLAATGRGHRTDAALEKTFGKRRLEIEWHPEIVHPRHTNAMRFEALDNSGNVIADWM
                     AYSIGGGAVTDDSGLRETGDVYPHENMDDILGYCNGKGITLWQYVVESEGNEIMDYLG
                     EIWSVMEAAIKRGLATEGVIPGGLNLQRKANSYMRKAHQFRNIFRTRATLYAFALAVS
                     EENACGGVIATAPTCGSCGVLPAVLKYSKQFNRHTNSDIIKALATAGLIGTLVKRNAS
                     ISGAEVGCQGEIGTACAMASGALTQLYGGTNYQIEYSAEMGIEHHLGLTCDPIAGLVQ
                     IPCIERNVFAASRALAHNTFAMLSDGRHMVSFDKVVQTMKQTGKDLPSLYKETAAGGL
                     AFFSADLHVDD"
     gene            complement(2670..3749)
                     /locus_tag="PKK03_04335"
     CDS             complement(2670..3749)
                     /locus_tag="PKK03_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNX83655.1"
                     /translation="MSIELRKDYAYSLLVPTSMGVRITPVNHQPVHTSDTFFMQATSA
                     ETNVASVASYLGLPVKVLTTFVKDSPIAQFIRNNLSGRHMTYEGKEVAQGGPWGYRHQ
                     FNIADMGYGSRGPRVHNDRAGEVGRTLNAKDFDLDRIFGKEGVQIVHLSGLIGALSPE
                     TSAFCLELAREAKKHGTRISFDLNYRASFWKGREKELSGIFREIASVADILVGNEEDF
                     QLCLGIEGPEAGGKDLGSKMETFKGMIDRVRREYPGASVFATTLRQVLSVDAHLWGAI
                     MWEGGNWHIIEPREIHVLDRIGGGDGFVGGLLYGILKGWDPARWVEFGWASGALATTF
                     LTDYAQPSDEEMVWSVFEGNARVKR"
     gene            complement(3856..5202)
                     /locus_tag="PKK03_04340"
     CDS             complement(3856..5202)
                     /locus_tag="PKK03_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNX83656.1"
                     /translation="METSGDNGIDFRHPELWDEPDYIRKEMARAMGHIYRHGMTTTSG
                     GNMSVTDSLGNTWITPSGIDKGSLKPDEVVCVAPDGTWAGPLRPSMEYPLHRGVYAAR
                     PDIRALIHAHPPVLTSFSIVHRIPDTSAVRAWRETCGAVGYAGYAVPGSDAMGEKVAR
                     EFIKGHNAVIMENHAVVVGGRDLAEAQARLEALEYCARTIHAAGFLGEPVITENATGD
                     NSEFNSGNYPPKTGRGLLTGLTDSEMSPDEELLAEEICRTAVRACRRELMYGYCGIVS
                     ARSTGDNVLITREKVLRCNIGRGDITEVRSGEIHSFPGERLHAEIYQRFPAVRAVITA
                     RPPYLMAFAVTGKEVNVRTIPESWILLQEMPLVPFGALSPGREEISGKLSAGIPAVLI
                     SNDSVLVTGGSLLQAFDRLEVAEMTAMSLVLGKSLGSVNPISDNNITELRRAFFSD"
     gene            5278..5913
                     /locus_tag="PKK03_04345"
     CDS             5278..5913
                     /locus_tag="PKK03_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83657.1"
                     /translation="MAVTLSEYDLPDAFLFSEGSTGIIVWQPQETIIVLGQSNSVESS
                     ICTEAVAAAPLRVTKRPSGGETVILTPATVAFTVARQFPAMIQFREFFRMVNSVVIDG
                     LRELGVTSLGSKGISDITIGNRKILGSSMRKVGDKLVYHAVLNLAEDPALFSRYLLHP
                     RREPDYRAGRSHHEFVTSLAGEGYSFAPGEIMEMLNRRLGGLMVSRPAVAQ"
     gene            complement(5933..7831)
                     /locus_tag="PKK03_04350"
     CDS             complement(5933..7831)
                     /locus_tag="PKK03_04350"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNX83658.1"
                     /translation="MTKNLFIIALATALLLGCSTTARTGKTEVEDPAAAAANAAEIAA
                     RARAAETLAAIPCRCDSIGNNPPPVKFLRLPEPLTHQPQFDILCKSEYPATVSINGQT
                     VKQYKTGIFFMTVPFNEGVNMITAEAVYPDGRKAVCEHAVIYEKRDLSRQPFPLWIEK
                     RSFEPSADLEVLPGEVVKISFRASKGQEAWLDIKPGRKSIKCLREDYNDYSLYRAEVP
                     VRDFAAGVPHTFAVRLVPATGGAPEGVYRPELSRTVTVRNPEDFPLVRVKNDYSRLVY
                     NLGAPRLGGPIRSELGPGVILKTSGKIGNNYRIRLSNTETGFISEDDVEIQDGGKTQP
                     LYNVTSMSCGPSARGDVVSIPYLEPVPYEVFPDPDGKRLVVTLFGVESAATWVTHRKG
                     CRVVDKLTWEQSTPETFRVYVNLKSGKIWGYDIRPAGRSLMLTVRYAPVFDPAGEKPL
                     AGLKIAIEAGHGGSNSGAIGLSGLVEKDINLDLSFRLGELCDSMGAEVLQVRESDVDM
                     TLLDKRDKAIKAGADMLISIHANAGGTGYLSVEGTSTYWHNPFWAPLAQTIYDRMLET
                     GLAEFGVVGSFNYTVTRASQMPAVLVEQAFMSHAEDEEKLADPEFRQLMAVKIFEGMV
                     GYLKIMQE"
     gene            complement(7828..10614)
                     /locus_tag="PKK03_04355"
     CDS             complement(7828..10614)
                     /locus_tag="PKK03_04355"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNX83659.1"
                     /translation="MTWKFTFRVIAAVMAIAIAGCATQKKAVTGDPSGRTPGAEREFR
                     AAWVATVANINWPSRPGLTVEEQKSEATALLDLLHRNNFNAVIFQVRPHCDAMYRSDL
                     EPWSYYLTGEEGKAPDPYYDPLQFWIDEAHARGIELHAWLNPYRAHHPAAGPVTDVSI
                     VRKRPDLVLKLEVENYWWMDPALKGTQDHSYNVVMDLVKRYDLDGIHFDDYFYPYPDY
                     NNFKDFPDDQSWKAYQGSGGKLSRSDWRREAVNTFIERLYKGIKAEKPWVRFGLSPFG
                     IWQPYNPPAIGSGFNQHETLYADAKLWLNKGWIDYYSPQLYWPINQIAQSFPVLLGWW
                     KDENLKGRHLWPGISIGLSPASRAIDETINQIMVTRGVLPGSPGVIHWSIGPLVSSPG
                     LARAVADGPYRRPALVPPMPWLDRKAPAPPAVNRKAENGTLRLSWTYPDHADIGRWVV
                     YYKYGPQWNQHIHGSTITEDAIPAFTLNRTYLARTSRDKVTKADQAFTSLDSVAVSAV
                     DRFGNESGISRMGVNEFTLADAPDLDKSLTEFYEGMKRPPVPVPAVTPGINVLLDEHP
                     DLIRGKRVGLITNPSAVGIDMRSTVDILATTPGVNLVALFGAEHGVRGAQHGRIFTDG
                     EKDPVTGIPVYSLYGESWAPKREWLDSIDVMLFDIQGVGSAWYTYKFSMSHAMEACAK
                     AGVPFIVLDRPNPLGGRVVEGPMHDTISIYRHRLPLRHGMTHGELAKMWNETEGYGAD
                     LTVIKMKGWNRSMMWNETGLQWVMPSPNIDNWETAVVYPGQCLFERTNMSEGRGMTKP
                     FLVTGAPWVNADKAAADLNSRGIAGAYFRPLYFIPRSAGTGYNRSGKPWNELCGGVEI
                     ILTDPAAFRSVEASLHIIDAYRKTSPDSLVWSPPVIMRRLNEPGVTVQEVVKACQDDV
                     KEFMETRQKYLLYR"
     gene            complement(10880..11644)
                     /locus_tag="PKK03_04360"
     CDS             complement(10880..11644)
                     /locus_tag="PKK03_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HNX83660.1"
                     /translation="MRNIAQRHKIILAELEKDGYVRVHDLSLRLGVSEVTIRKDLKDL
                     EERRMLIRSHGSAGQVSSLTIDRHIDEKEKVQVGEKTRISEAANRLLVKNDRIIIASG
                     TTVLAFAQHITITDPITVITPSVKVSLILSYKPNVDIIQLGGSLRKSSASVIGPYAES
                     LLTEMMCSKLFIGIDGLDLDHGLTTSNIAEAHLNQYMINAAQEVIVLADSTKFGKRGF
                     GKICNLSTVHHIITDRNAPGNFIQIIREKGIEVTLV"
     gene            11848..13419
                     /locus_tag="PKK03_04365"
     CDS             11848..13419
                     /locus_tag="PKK03_04365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNX83661.1"
                     /translation="MEREDFLKDLKDKKRQWDFIIIGGGATGLGVAVDAVTRGYSTLL
                     LEQSDFAKATSARSTKLVHGGVRYLQKGDVALVREALRERGYLRRNAPHLVKNQRFII
                     GNYKWWEKPFYTIGLTFYDLLSGGMSLGRSLPMSRSTVVKSIPQIVRKGLKGGVVYHD
                     GQFDDSRLAVNLMQTAIEKGATVVNYTKVIQLLKTSEGQVSGVVAVDELDGTHFTAEG
                     KCIVNATGIFVDSIIRMDNPEEKPLVRPSQGVHLVVDRSFLGGDDAIMIPKTSDGRVM
                     FGVPWHNRVILGTTDVPVKEFVLEPKALDEEVDFILETAGRYLERKPQRSDVLCVFAG
                     LRPLAAPKHDDGGAKTKEISRSHKMLVSPSGLVTITGGKWTTYRKMAEDTVNAGIRAR
                     KLPFRPCRTRDLKIHGWYENPDRTNRLYIYGSDLDELMLMQKENPEWAEKLNGESDFT
                     AGEVIWAVRKEMARTVDDVLARRVRVLYLDARQSISLAPKVAGIMARELGRDKDWVRQ
                     QVEEYTNLAKGYVMS"
     gene            13540..15000
                     /locus_tag="PKK03_04370"
     CDS             13540..15000
                     /locus_tag="PKK03_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX83662.1"
                     /translation="MINFLKPPPYKEPLPEEKIDKTYKRLRLQVFMGAFIGYAAYYLV
                     RKNLALAIPGMIQPVESGGLGYTKLQLGIALSAISIAYAFSKFIMGALSDRSDARKFL
                     VIGLILSAVLMMSVGIFPFATSAVWIIFLFMLVIGWLGGMGWPPCGRIMVHWFSHNER
                     SFKMGIWNTSHTVGSGLMGNLAAWGTMLLGGLFLVDLGGGVTVEQSWRAVFIFPSAVA
                     LLLALFCWWALRDTPQSCGLPSIDKYRNDVPVQKEMSSDKKIPVKELFIKYIFTNRIL
                     WLIALANVFVYLIRYGIGDWAPTYCEEAGLLTSEQSKMAFSLHNYAGVPGMVLIGWIS
                     SKVFKGRCAPANVIYMVLVLLSVLLYWKAVPVASFFANAFGTDPDATRVTVVYSALIA
                     IGFFIYGPVALIGVQAVNLVPKNAAGTAAGFVGLFGYLIGDALLSKVLIGAVADNVSL
                     GWGATFWIFIAASILAAIFSATTWKKEKETYLGHAK"
     gene            15006..15935
                     /locus_tag="PKK03_04375"
     CDS             15006..15935
                     /locus_tag="PKK03_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HNX83663.1"
                     /translation="MKRLIVTVLAAAFCLTPTVNAQTYRHISGWSQDVNDRIEGFLNS
                     TITMNIRKVAVFDSDGTTFGQVPYYLADEALYRYADVVLKERKDSEAKDKLKILERMV
                     KDGNNVGKPYVEDRVHFLAGLTPGEIEMIGYECYRESYSRKTYPEMKQLVANLKEYGF
                     EVYILTASPEFLYQKFVSEEYGIPVTNVVGAKCVVKEGVTTGEIIPPIPQDDGKAHTI
                     ETFIKARPLIVGGNSRGDMDMLNESAGLKIVVNPDDITVRGPEDGPMNGHTVKSYWEK
                     EGALIVRCNDVRDPKVRFHTEEWKIRQNIANPK"
     gene            15945..17129
                     /locus_tag="PKK03_04380"
     CDS             15945..17129
                     /locus_tag="PKK03_04380"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNX83664.1"
                     /translation="MKIRILFSMAVTAALLAGCAGKNAPVTGGVQDYMPDNAVIAHRG
                     TIYWAPELTESAFRWARNTGADYMELDVHRTKDSVLVIMHDKTFKRTTNVAEVFPGRE
                     GDPIGTFTWEEIMQLDNGSAFNKKNPDQARQSFEGQNVLTFEDVFRIAEGKRIKRNPD
                     GSRVFTKDETGKYHFEYEADPADNGHRPGVYIEFKIPDEYPLIEEQVYDELARIGWNP
                     LEGVTPSADEPFLKGDRVNVGNTRGKILVQTFSQKGMMNIKRVFREQVPASYLIGNPK
                     TNLFAQEAVTDSIVAFARECGAQFIGTNLGDENDGLSPEFAAKIRATGLKSNVYSFNT
                     VEQMEKYFGQGDGKKASPLTEGMITNRTDLTIDFYHSRKVRAYKTEKSPQEILADLGY
                     NQ"
     gene            17142..19091
                     /locus_tag="PKK03_04385"
     CDS             17142..19091
                     /locus_tag="PKK03_04385"
                     /inference="COORDINATES: protein motif:HMM:NF027688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcineurin-like phosphoesterase C-terminal
                     domain-containing protein"
                     /protein_id="HNX83665.1"
                     /translation="MPDIKKLIFLLLIAAIAACTGCKEDPPPPDEGGLKITGVSIPAS
                     LNIPAGGEVTITGSGFAAGDQMVFVLVSDAGKVYTATVASVTGQSAGFTLPAGLTSGT
                     YSLTVKRGTDSMVLGTLTINVVADNAIPDKPGMTLKGVVYCDGEGIPGVVVSDGAEVT
                     VTDSKGIYYLPSLKQNKFVFISLPGNYEIAASDNIPQFFKRLAGGNSVEQHDFSLVHT
                     DNNNHVVLAMADWHLANRNDDLAQFSNGFLPDVNSTISSYTSAGKKVYGVPLGDMTWD
                     AYWYDNNFRLEQYLIEMKKVNCVMFNVMGNHDNDPYVQGDLPPEAPFREIIGPTYYSF
                     NLGQVHYVVLDDIQYINTGGAQGTIGERNYSDVVVSAQTSWLAKDLAAITDKSTPLVI
                     FIHAQLYGNPTLNAGGEEVHSLALNNGSTLLQMVQDFSEVHFVSGHTHINFTIDGGAV
                     MEHNTAAVCATWWWTGKTGYANNQVCKDGSPGGYGIWEMNGKDLKWHYKSIGKPSDYQ
                     FRSYDLNKVHITAAAFAPNSDDAALSEYAGVYASPNPVNDVLINVWGYDPEWTVEVSE
                     GGTPLTVERVTAMDPLHIISYEAKRLNAGATPTSSFVTGNTAHMFRVRASSATSTLEI
                     KVTDRFGRVYTETMTRPKDFTWSMQ"
     gene            19124..22450
                     /locus_tag="PKK03_04390"
     CDS             19124..22450
                     /locus_tag="PKK03_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX83666.1"
                     /translation="MKFKPDLKVILATFLMALVMLPVMAQERQVTGTVYEPDGVTPVF
                     GATVVLKGTTTGTSTDARGAYSIRVAGASPVLEIQFLGYIKQELAVGDRNVINVVLQE
                     EAIEVSAVVVTALGLTREEKSLGYAVSKVASEEITQVVTNNWLSGMSGKVAGLTFNSA
                     SSGPISSMRVTLRGDASLSYGANEALFVVDGVPIRSGTTATSSSSSYTNSGSDFPVDF
                     GNGASDLNPEDIESVSVLKGPAATALYGSRAANGAIVITTKSGRKTKGIGVTLNSSVV
                     VERPGYWPDFQTQFGSGSDMGLNEYNFWTLDPSLVGTTDYPPRNLSRYAWGEEFSEDK
                     LRYQYAGKNWETGEIVKTPFVYQDDWFTGIFQDGITYNNSVDISGSNGEGQNIRFSMT
                     DTRNKWILPNTGFQKQSYALSFESPVNKWIDFKTKVNYYRTDSDNMPMAGYHQTTVMY
                     DLMWGYNSNSMADWRNEYFKGRFNAINRAATISEGGNSLVFAGTGSYNPYRVLYEELN
                     TTDKDRVFGNLGLTIKIARGLTLDLRTGMDFGNEFRTQRKPKMTFGWENGFYREQGFM
                     TFEANSDFLLKYERSFLDSRLTTTTAFGGNNMSSHYRSNTVQLDELDIEGVYHIDNVP
                     FGIYQRPSSFRNYKMINSLYGFMTLGWDDTYYLDITGRNDWTSTLAPKNWSYFYPSVA
                     LSVLVDQMFDFRTTLPAVSMAKLRLSWANVGNDTSPYGLDQYYSASSISGGYTLPSLI
                     PDPMIEPENMESWELGLDTKFFKNRLGLDIALYRSSTTNQIISLDIDPIVGASSMRIN
                     VGEITNKGIEMSMYATPVKTSDFTWNLNFNWAANRNTLVSLHESLDPSQPYEHDMGTT
                     IGGRLHLYSYVGQEMYQLYGFALKRAPEGSFYTDNNGERVDCSGQVLIDATTGLPSVE
                     STADHFLGKVNPDWKGGFGTSLRYRNLSLSALFSYQMGGHRFSVTHGILSYQGKLTNS
                     LEGRYDGIVAEGVNMTGTGEAATFQPNNTVTNSIYTYYQAITLDRYNGEAHTFETSFL
                     KWKEVRLEYALPSVFIQKTRVLQGASVAIFATNLFSWDKWPQFDPEGGMLTGTNVFNG
                     IEAGAYPMTRTVGANLRLSF"
     gene            22468..24045
                     /locus_tag="PKK03_04395"
     CDS             22468..24045
                     /locus_tag="PKK03_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNX83667.1"
                     /translation="MKNIKILIICGLVLGSLYSCTGKFEELNTNPNKTLVGMIQPSGM
                     FEPILYNSANQWLNRTHYYNNELTQFTAFTGGVTRREHQYFLEDSRDWYGFWNHYATY
                     GKNIDHMYELAIEHGDRSLEAIALTFKVLFFSNMTDMFGSIPYEEAFSARKPGGTLTP
                     KFNTQREVYEFLFEELEAANDIYATNPVFLNPAMDGMYGGNMAAWRKFNNSLYLRLLC
                     RVSGRTEMSVGTRMNAILDNPDKYPVFTSNADNATVKFTGTDPYISNFGTTTESEFTS
                     SGRKLTQQLIRLTVQTDDLGNQVYVDPRLPVIGKKNPNAQVNPANVWKGTVSGCTDIE
                     RSEVDRGTSWLNFKVFCRRDAPATYMDYAEVEFILAEAALKGWISGGQAEAKTHYEKG
                     VDASMKKWSEQGAFSDPAITITNADIQTYLASPLGSWDLATSKEEFLGNQKWLALFWV
                     GMEAYHEYRRTGYPVLTIGEGTIYNDKVFPTRFGYPNITMLTNADNAADALSGMGGEN
                     DMKTPVWWSKQAIAGTK"
     gene            24065..25315
                     /locus_tag="PKK03_04400"
     CDS             24065..25315
                     /locus_tag="PKK03_04400"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HNX83668.1"
                     /translation="MKNKNITNFGRTALIVLPLFLLSLFLLYSCKEDPVQEDPFFSLE
                     GSPTGLTLTKAAKTTSYVVRSNRPWEVVKKSGADWLKVFPEKGDADGIFKIYVTENPL
                     FVARTENLAFIVDGTEQPVLFRVDQAGNVPYITLPATVTVPAGGGPFSVDVVSNVTWT
                     YSLSDDSWLVEGSVTAQTLNLVAEMNTSDDPREVTLTLTSVDFPDVSESVVLTQSSGK
                     VVLEEDFNWLNYGNVVFYTTTNETRIDNWTQDQKDRGWTSTINTVTGSGNTAMLYARP
                     GFVKVGKTSYGGDLISPAFAKLIGTQTVTVTFKAIPYMTKAGAMDENVLKVSVIGPGT
                     VSQEQFIIDNWPVYPADDGAHTDYCVGMWSAPEATRTFTITGATSETQIKFLGGDYDL
                     TTEAGLPTRKNRIFLDDIKVEIVL"
     gene            25428..26330
                     /locus_tag="PKK03_04405"
     CDS             25428..26330
                     /locus_tag="PKK03_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83669.1"
                     /translation="MRKFFVPPTLVCVLALAASCSTILNMERTYPLEALLPADSNRFL
                     FVNFYDYQVPDFIRDRHEDGYAAAVKGFAMGLSSTIQTDPRALFMIGDSLRKGFTVIS
                     MQYPEFSDTVRALCSRYGANLLVALDSIRLLVDWDFYLAENDEGGSMLAKDFYLFSDT
                     YMTLYSADGEVIDRCAGEKNTFVKSKYTIFGMIGGPNIGNQKERIKLLTGSAAQDCIG
                     KYFPFTEGYTGKLFSGGELTKLNLLITGGKPEEAVGPLTELINRSSDPMASKAEHNLA
                     IANEIIENRKEREEIWRKYTNPEK"
     gene            26503..27891
                     /locus_tag="PKK03_04410"
     CDS             26503..27891
                     /locus_tag="PKK03_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83670.1"
                     /translation="MKRIISVISALVVTGSLFAGGLVTNTNQSALYTRLQSRNASTGI
                     DAVYYNPAGLTKLSDGFHLSLNNQTIGQTKTITTNYIYLNGNPKEYVGKVSAPFFPGI
                     YAAYKTGKLALSLGFNPVGGGGGAKYDNGLAMFEMQIADLKPLLTGAGLTTTNYSVET
                     LSFEGSSIYMGPQVNVSYEIHEMFSFAAGLRVVFASDKYLGAIENIMINPVHPLVNPT
                     GAMISAPAFFTTIGQPARAAQTSNTYADAVMKGTGFTPILSANFAPVGDLLNIAVKYE
                     FKTKLDLTTTVNDNKTAGLFIQDSVAVKDMPAMLSLGVDVKPSERFLISGSLNYYFDK
                     NVDYDGMPDVNVEMIDKNFIEAGLGIQVGITDRFFASAGWLTTVTGVNDNFQNDLKFS
                     LNTNTFGGGIGFRINDMIDLNLGGSYTIYEEASETYTHVITTTPILVQAPAINTYNNK
                     TWVAAIGLDFHF"
     gene            complement(28013..28693)
                     /locus_tag="PKK03_04415"
     CDS             complement(28013..28693)
                     /locus_tag="PKK03_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83671.1"
                     /translation="MKQIVLTVMLAALMNIAAGQSVESPNYGLKSPLTAEIVRVDFNA
                     DATVVWLSIMSDINNAWFCIDRNTFLVKPDGVRLKLTGLKGLPYCPATYKFKRPGEKT
                     SFSLTFPATGVLPWFSIVEECVGGCLSFRGVVTDAGLNGKLNEAFALSGAGSSMAAYR
                     IYEEIINETDSLNLGIEGSVYTSLIMLDRSMGRNEAARQWYNRMMTSHTPDLKLYLEN
                     LKRQGAEY"
     gene            28889..>29177
                     /locus_tag="PKK03_04420"
     CDS             28889..>29177
                     /locus_tag="PKK03_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83672.1"
                     /translation="MKILPVVIGIMMLTAVPCAAQESTDFKPSGKFFGLLFANYHTTF
                     SGGDNNSAFEVTRSYFGYDFSFSREISSRVMYDGTTEVINGKTIYSGYLRNA"
BASE COUNT         7237 a         7713 c         7436 g         6791 t
ORIGIN      
        1 aggcaaagaa tgcaggaaag catattcctt cactcaaaga tgccttcttt gttgacgacg
       61 aggctgccca taggggttat gtcacctatt cggaacctaa tgccggtaaa actactggtt
      121 ttcaggcagg gaaacacgaa tggttcccct tcccctacag tgtgacttcg attaatccca
      181 acctgcagca aaatccggga tgggaataaa cttcaaaaac tttaaaactg gaagaagggt
      241 tcagattatg ttaaattcaa atccggctgg aagggatatc aatcactcat tacttgccat
      301 cccgctggca gaaggagaca gaaatccgaa tttttaatca gtaagtgagg tttggttcga
      361 aagagggtgc catcgatgac accctctttc tgttttctat cccggctgtg tcttttctat
      421 caatcctgac aaaagtcttc ccgggcctgc atcacatgca atagcaatgt tttttctgcc
      481 ggccgtttca gtcactttca gccggcttcc cttaaatcaa cagaaatact cagagtatgg
      541 tattgcagta ctcgaccagc tttatgacag ccttgtagtc gtcaaagtcc agcctggttt
      601 ttgccagata ctgtttgagt tccttccccc tttccgggaa gatctgcagg aactgacgct
      661 tgttgtcaaa gtttttcatt tccccgtctt tcagaaccca gtactcagtg tcgtcagtta
      721 tcttaaactc atccggcagc ttaaggttga ttgctccgtt gttgtcatag actgccttta
      781 catagttata gcttgtggtt tgtgacatcg ccccgtaacc ggttggaact cctgctgact
      841 ccacatatgc cttgtgttgt atgaaaaggg tgacagggcc tctcagtgcc agctcataga
      901 aacctttctc gaatggtatg aatgtccggt tagccatgta aaccgtatct atcagctgag
      961 ggtcattcag cacaaagaac tggtcacgtt gcatgaacac catctcctgg tcaacgatgt
     1021 tataattgag tactgccctg cgcttctcgc ccgacttcat cttcaccacg ctggtaatga
     1081 atttgggaag caggaggttt ggcaggttct gcaggctgtc tctctgggca tatgctcctt
     1141 ctgccacaaa caggagagca aggaagatat ataaaaccct ggctttgttc ttcatttcaa
     1201 aagtgttaag ttggaaatca gattatcagt cagtgataaa ggtaaataaa taaatctgaa
     1261 ggagcacagc cctcagtaga attttgaact gcaagtgctg gctggcctgg ttcgtgagcc
     1321 cctgcccggg aggcccctcc cgggggaatg ttatcctgac tgtacagtcc tgtttcagaa
     1381 tttgttatta catttgcggc ttctacagta gaaaagaaca actctgctga attatcatgg
     1441 aaactataag aaacctttac cgcatcgggc ggggaccgtc agcaagccac accatcggcc
     1501 cggcgagggc tgcccagagt ttcatggcac agaatgaagg tgccgcagca tacagggtgt
     1561 cgctgtacgg aagcctggcc gccacgggaa gggggcaccg gaccgatgct gcccttgaaa
     1621 agacctttgg aaaaagaaga cttgaaatag agtggcatcc ggagattgtc catccccggc
     1681 ataccaatgc aatgaggttc gaggcacttg acaacagcgg caacgtcatt gccgactgga
     1741 tggcttacag catcggcgga ggagccgtga ctgatgacag cgggctgagg gagacaggcg
     1801 atgtctatcc tcatgaaaac atggatgata tcctcggtta ttgcaacggt aaagggataa
     1861 cactttggca gtatgttgtt gagtcagagg gaaatgaaat catggattat ctcggggaga
     1921 tatggagtgt catggaagct gccataaaaa ggggacttgc gactgaaggt gttattccgg
     1981 gcggactgaa cctgcagcgc aaggctaact cctacatgcg caaggcacat cagttcagga
     2041 acatattccg cacccgggcc accctttatg ccttcgccct ggcagtgagt gaggagaatg
     2101 cctgcggggg agtcatcgca actgccccca catgtggatc atgcggtgtc ctcccggccg
     2161 tattgaaata ctcaaaacag ttcaacagac acactaacag tgatataatc aaggcgctgg
     2221 caacagccgg gctcataggc acacttgtaa aaagaaatgc atccatctcc ggagccgagg
     2281 ttggctgcca gggtgagata ggcactgcgt gtgcaatggc ctcaggggct cttacacaac
     2341 tttacggagg cacgaactac cagatagagt actccgcaga gatgggaata gagcatcacc
     2401 tgggtcttac atgcgatccc atcgcaggtc ttgttcagat accctgcatc gagaggaacg
     2461 tatttgccgc ctcgagagcc cttgcgcata acaccttcgc catgctctcc gacgggcgcc
     2521 acatggtcag ttttgacaag gtggtgcaga ccatgaaaca aaccggaaag gacctgccca
     2581 gcctctataa ggagactgcc gcggggggcc tcgccttctt cagcgccgac ctgcacgttg
     2641 atgattgatt tatccggagg aaactctttt cagcgtttga cccttgcatt gccctcgaag
     2701 acgctccaca ccatctcctc gtcggacggt tgggcatagt cggtaaggaa ggtggtagcc
     2761 agcgcaccgc tggcccagcc gaactccacc cacctggcgg ggtcccatcc cttcagaatg
     2821 ccgtaaagca atccacccac aaagccgtcg cctcctccga tgcggtccag aacatggatc
     2881 tcgcgtggtt ctataatatg ccagttgcct ccctcccaca tgatggctcc ccagagatgg
     2941 gcgtcaacac tcagcacctg ccgcagcgtg gtggcaaaga ctgaagcccc cgggtactcc
     3001 ctcctgacac ggtcaatcat ccccttgaag gtttccatct tggagccgag atccttccct
     3061 cctgcttcgg gaccctctat tcccaggcaa agctggaaat cctcctcgtt gcccacgagg
     3121 atgtcagcca cggaagctat ctcccggaag atgccggaga gctccttctc acggcctttc
     3181 cagaacgatg cccggtagtt gaggtcaaac gatatccgcg tgccgtgctt cttcgcctcg
     3241 cgggccagtt caaggcagaa ggcgcttgtc tccggcgaca gggctccgat aagacctgag
     3301 agatgcacta tctgcacccc ctccttcccg aagatacgat ccaggtcaaa atccttggcg
     3361 ttcagtgtgc gccccacctc cccggcacgg tcgttatgca ccctcgggcc gcgggagcca
     3421 tatcccatgt cggcaatatt gaactggtga cggtatcccc aggggccgcc ctgtgcaacc
     3481 tcctttccct cataggtcat atgccggcct gagaggttgt ttctaatgaa ctgcgctatg
     3541 gggctgtctt tgacgaatgt tgtcagtact ttcaccggaa ggccgagata gctggctacg
     3601 cttgccacat tagtctccgc gcttgttgcc tgcatgaaga aggtgtcact tgtatgaaca
     3661 ggctggtggt tcacaggggt tatgcgcacg cccatgctcg tggggacaag caatgagtag
     3721 gcataatctt ttcttaattc aatgctcatt ttctattcgg tttttcagat ctctgtgtta
     3781 taatgctgca cataaatcac cctgccggca gggtaaaatt atcacttttt cctgaccggc
     3841 aggtggaatt tccggtcaat cgctgaaaaa ggcccttctc agctccgtga tgttgttatc
     3901 tgaaatcgga ttgacgctcc cgagtgattt tccgagtaca agtgacattg cagtcatctc
     3961 ggccacctcc agccggtcaa atgcctggag caggctgcca ccggtaacaa gaaccgaatc
     4021 attggatatc agcacagcgg ggatgcccgc actcagtttt ccggatatct cctcccggcc
     4081 cggtgaaagt gccccgaaag gtacgagagg catctcctgc agcaaaatcc agctctcagg
     4141 gatggtcctg acatttacct ccttccctgt caccgcaaaa gccatcaggt acggaggccg
     4201 ggcagtaatc accgccctga cggcgggaaa cctctgatag atctcagcat gcagtctttc
     4261 cccgggaaag ctgtggattt ctccgcttct gacttcagta atgtcacccc tcccgatatt
     4321 gcatcttaaa accttctcac gggtaatgag tacattgtct cctgtgctcc ttgccgatac
     4381 aatcccgcag taaccgtaca taagctccct cctgcatgcc cgcaccgcag tacggcaaat
     4441 ctcctccgcc agcagttcct catcagggct catctcactg tctgttaacc ctgtcagcaa
     4501 acccctgccg gttttcggag gataattgcc ggaattgaat tcagaattgt ctccagtggc
     4561 attttcagtg atcacgggct ccccaaggaa tccggcagca tgtatcgtcc tggcacagta
     4621 ttccagggcc tccagtcgcg cctgcgcttc tgccaggtct ctgccgccga caaccacggc
     4681 atggttttcc atgataactg cattgtgacc cttaatgaac tcccgggcca ctttctctcc
     4741 catggcatcg ctgccgggaa ctgcataccc ggcatacccg actgctccgc aagtctccct
     4801 ccatgctctc accgccgatg tgtcaggtat ccggtgcaca atgctgaagg aggtcagtac
     4861 cggcgggtgg gcatgtatca gcgccctgat gtcaggtctc gcagcataga cgccccggtg
     4921 gagcgggtat tccatggatg gcctgagcgg gcctgcccag gttccgtcag gggcaacgca
     4981 caccacctcg tcgggcttca gtgatccctt gtcgatgcct gacggggtga tccaggtgtt
     5041 tccaaggctg tcagtgacag acatgttacc ccccgaggtg gttgtcattc catgcctgta
     5101 gatatggccc atggctcgtg ccatctcctt ccggatataa tccggttcat cccatagttc
     5161 aggatgtctg aaatcaatgc cattatcccc ggatgtctcc atggtgcagg ttttttattt
     5221 aatttggaga agttttctcc ggaacacggt ataaagatag caagaagatc tctctgcatg
     5281 gcggtgacac tctctgaata tgaccttccc gatgccttcc tgttcagcga gggctccacg
     5341 ggaataattg tctggcagcc gcaggaaaca atcattgtgc tcggccagtc aaacagcgtg
     5401 gaatcgagca tatgcacaga agcagtggca gccgctcccc tgcgcgtcac caaaaggccg
     5461 tctggcggag agacggtcat actcacccct gccacagtcg cgtttaccgt agccaggcag
     5521 tttccggcca tgatacagtt cagggaattc ttcaggatgg tcaacagtgt ggtaattgac
     5581 gggctgagag aattgggtgt taccagcctg ggttcaaagg gtatctctga catcaccatc
     5641 ggcaaccgca agatactggg ttcatcgatg aggaaggtag gcgacaagct ggtgtatcat
     5701 gctgtcctca acctggcaga ggatcccgcc ctgttcagca ggtatctgct tcatccccgc
     5761 cgggagcctg actatcgcgc cggcagaagt catcatgagt tcgtgacctc acttgccggc
     5821 gagggttaca gcttcgcccc cggcgagatc atggaaatgc tgaaccgccg gctcggagga
     5881 ctcatggtct cccgtccggc ggttgcgcaa taaaatgaat tatagcgaag gcttattcct
     5941 gcattatctt caggtagccc accatcccct caaaaatctt caccgccatc agctggcgga
     6001 actccgggtc agcaagtttc tcctcgtcct cagcatgact catgaaggcc tgttcaacca
     6061 gcacggcagg catctgggat gcacgggtga cggtatagtt gaatgatccc accacgccga
     6121 actccgccag ccctgtctcc agcatcctgt catagattgt ctgtgccagc ggcgcccaga
     6181 acggattatg ccagtaggtg ctggtgcctt ccactgacag gtagccggtg cctcccgcgt
     6241 tggcatgaat gctgatgagc atgtctgccc ctgcctttat ggccttgtcg cgcttatcga
     6301 gcagggtcat gtccacatca ctctccctta cctgcagcac ctcggcgccc attgagtcgc
     6361 aaagctctcc gagcctgaat gacagatcga ggtttatgtc cttctcaacg agacctgaca
     6421 acccgatagc gcctgagttt gacccgccgt ggcccgcctc gatggcaatt ttcagtcctg
     6481 ccagcggctt ctcccctgcc ggatcaaata ccggggcata cctgacggta agcatcaggc
     6541 ttctgccagc gggtctgatg tcatatcccc agatcttccc gctcttcaaa ttcacgtaaa
     6601 cccggaaggt ttccggggtg ctctgctccc acgtaagctt gtcaacaacc ctgcatcctt
     6661 tgcggtgggt gacccacgtg gccgccgact ccaccccgaa aagcgtcaca acaagacgtt
     6721 tcccgtcagg atcgggaaaa acctcataag gcaccggctc aaggtaaggt atggaaacca
     6781 catcgccccg ggccgacggt ccgcatgaca tgctggtgac attgtacaaa ggctgagttt
     6841 tgcccccatc ctgaatctca acatcatcct cgctgatgaa tcctgtttcg gtattgctca
     6901 gcctgatcct gtaattgttg ccaatcttgc cgctggtctt aagaatcacc ccgggtccca
     6961 gttctgacct tatcggtccg cccagtcttg gggctccgag gttataaacg agcctcgagt
     7021 aatcattctt cacgcgtacg agcggaaaat cctcagggtt ccggacggtc actgtcctgg
     7081 acaattcagg cctgtagacg ccctccgggg ctcctccggt tgcaggcacc agcctgacag
     7141 caaaggtatg cggcacgcct gcggcgaagt ccctcaccgg tacctctgcc cggtacaggc
     7201 tgtagtcatt ataatcctcc cgaaggcatt taatgctttt ccttcccggt ttgatatcaa
     7261 gccatgcctc ctgccccttc gaggcgcgaa agcttatctt caccacctcg ccgggaagca
     7321 cttccaaatc ggccgacggc tcgaatgacc tcttttcgat ccatagcggg aatggctgcc
     7381 ggctcaggtc tctcttttca tagatgaccg catgctcgca aactgctttc cggccatcgg
     7441 gatagaccgc ttcagccgta atcatgttca ctccttcatt gaatggaaca gtcataaaga
     7501 atattcctgt tttgtactgc ttcacggtct ggccgttaat gcttacggtg gcagggtatt
     7561 cacttttgca caggatgtcg aactgtggct ggtgcgtcag cggctccgga agcctgagga
     7621 actttaccgg gggcgggttg ttcccgatgc tgtcgcatcg gcatggtatt gccgcaagcg
     7681 tttctgcagc cctggccctg gctgcaatct cagccgcatt cgcagccgcg gctgccgggt
     7741 cctccacctc agtcttccct gtcctcgcgg tggtgctgca tcccaggagc aacgccgttg
     7801 ccagggctat gataaacagg ttcttcgtca tctgtagagc aggtatttct gtcttgtttc
     7861 cataaactcc ttcacatcat cctggcaggc tttcaccacc tcctgtacgg ttaccccggg
     7921 ttcattcagc ctcctcatga tgaccggagg tgaccagaca agtgaatcgg gacttgtctt
     7981 cctgtaggca tcaataatat gaagtgatgc ttctactgag cggaacgcgg caggatctgt
     8041 caggatgatc tccactcctc cgcacaattc gttccacggt tttccgctgc ggttataacc
     8101 ggtgccggct gagcggggaa tgaaatacag aggcctgaag tatgccccgg cgattcctct
     8161 cgagttcagg tccgcagcag ccttatcagc attgacccat ggggctcctg tcaccagaaa
     8221 aggcttggtc atgcccctgc cttctgacat gttggtacgc tcgaagaggc actggcccgg
     8281 gtataccact gccgtctccc agttgtcgat attgggtgac ggcatcaccc actgaagtcc
     8341 tgtttcgttc cacatcatgc tcctgttcca tcccttcatc ttgatgacgg tgaggtcagc
     8401 tccgtagccc tcagtctcat tccacatctt cgccagttcg ccgtgagtca tcccatgcct
     8461 aagcggaagg cgatggcggt agatgctgat tgtatcgtgc atcgggccct ccacaacacg
     8521 gccgccgaga gggttgggcc tgtcgaggac gataaatggt acgccagcct ttgcacatgc
     8581 ctccatggca tgagacattg aaaacttgta tgtgtaccat gctgatccca ctccctggat
     8641 gtcaaagagc attacgtcga tactgtcgag ccactcacgt tttggcgccc atgattcgcc
     8701 gtaaaggctg tagacgggaa tcccggtaac aggatccttc tcgccgtcag tgaagattcg
     8761 cccgtgctgt gctcccctga cgccgtgttc cgctccgaag agagccacga ggttaactcc
     8821 gggggtggtg gcaagtatgt caacagtgct tcgcatatct ataccaaccg cggatgggtt
     8881 ggtgatgagg cccacccttt tgcccctgat caggtcggga tgttcgtcta aaagcacatt
     8941 tatacccgga gtcactgccg gtacgggtac cggaggccgt ttcatccctt catagaactc
     9001 tgtcaggctt ttatcaagat ccggtgcatc ggcgagggtg aattcattga cccccatcct
     9061 gctgatgcca ctttcgttcc cgaaacggtc aacggctgaa acggctacag agtcaagcga
     9121 agtgaaagcc tgatctgcct tcgtcacttt gtcacgggaa gtgcgggcaa ggtatgtgcg
     9181 gttcagtgtg aaagccggta tggcatcctc agttatggtg ctgccatgga tatgctggtt
     9241 ccattgcgga ccgtatttgt agtagaccac ccagcggcct atgtcggcat gatcaggata
     9301 ggtccatgac agcctcagtg taccgttttc ggcctttctg tttaccgcgg gaggtgccgg
     9361 ggctttcctg tcgagccatg gcatcggtgg taccagtgca ggcctgcggt acggcccgtc
     9421 agcgacagcc ctggccagcc ccggggagct gaccaatggc ccgatgctcc agtgaatcac
     9481 gccgggactc ccgggcagga caccccgtgt gaccatgatc tggtttatgg tttcatcaat
     9541 agccctcgat gcaggcgaca gcccgatgct gatcccgggc cacagatgac gccccttaag
     9601 gttctcatcc ttccaccatc cgaggagcac cgggaaactc tgggcaatct ggttgattgg
     9661 ccagtaaagc tgaggcgagt aatagtcaat ccatcccttg ttgagccaga gcttcgcatc
     9721 tgcgtagagt gtctcgtgct ggttaaagcc gctgccgatg gcaggaggat tgtaaggctg
     9781 ccatatcccg aaggggctga gcccgaacct gacccatggc ttctcggcct tgatgccctt
     9841 atagagccgt tcgataaagg tatttactgc ctccctgcgc cagtcggagc gcgaaagctt
     9901 cccgcctgag ccctgatagg ccttccagct ctggtcatca ggaaaatcct tgaagttatt
     9961 gtaatcggga tatggataaa aatagtcgtc gaaatgaatt ccgtcgaggt catacctttt
    10021 taccaggtcc atcaccacat tgtaggagtg gtcctgggta cctttcaggg caggatccat
    10081 ccaccagtag ttctccacct ctagcttaag caccaggtcc ggacgtttcc ttacaattga
    10141 aacatctgtt accggtccgg cggcgggatg gtgtgcacga tagggattga gccatgcgtg
    10201 gagctctata cccctggcat gagcctcatc aatccagaac tgcagagggt catagtaagg
    10261 atcaggggct ttaccctcct cgccggtcag gtagtatgac cacggttcaa gatcgctgcg
    10321 gtacatggcg tcacaatgcg gccttacctg gaagatgacc gcgttgaaat tgttccggtg
    10381 cagcaggtcg agcagcgctg ttgcttcgct tttctgctcc tccacggtaa gacctggtct
    10441 gctgggccag ttgatattgg caacagtagc cacccaggca gcgcgaaatt cccgttcggc
    10501 gccgggcgtg cggcccgacg ggtcacctgt cacggccttc ttctgggttg cacaaccggc
    10561 aatggcaatt gccatcaccg ctgcaattac tctgaatgtg aatttccatg tcatagaggt
    10621 ttggttttga aggtgtactg atacaaatat aaaaatcctc cccggctgac atacagtccg
    10681 ataaagaata ataagagatt atacaacata tatcatacaa attaagcagg cagcaggcag
    10741 actgagactg cagcggcagc ggaggatcag tcccgcatcc gccagttggc ggaccgggca
    10801 ggctgtagtg gatttgagat ttccgaattg tggaaatcac agatcgcaat tcgcagatag
    10861 cagatcgcaa ttcgcaaatc tacaccagcg tcacctctat ccccttttcg cggatgatct
    10921 ggataaaatt accgggtgcg ttgcggtcgg ttatgatatg atgcaccgtg ctgagattac
    10981 atatttttcc gaagccccgc ttgccgaatt tagtggagtc ggccagcaca atcacctcct
    11041 gggcggcatt gatcatgtac tggttgagat gagcttcggc tatattactg gtagtcaggc
    11101 catgatcaag gtcaagaccg tcaataccta taaagagctt gctgcacatc atctccgtca
    11161 ggaggctctc agcgtaggga ccgatgaccg aggcagagct cttgcggagt gatccgccca
    11221 gctggataat gtcaacattg ggtttatagc tgaggataag tgaaaccttt accgaggggg
    11281 tgatcacggt tatcggatcg gtgattgtga tatgctgcgc aaaagccagg accgtggtgc
    11341 cggaagcaat gatgatccgg tcatttttga caagcagtct gttggctgcc tcggaaatcc
    11401 tggtcttctc tcctacctgc accttctcct tctcgtcaat gtgacggtca atggtaagcg
    11461 aactgacctg gccggcactg ccatgactgc ggatcagcat cctccgttcc tcaagatctt
    11521 tcaaatcctt tcgaatcgta acctcagaaa cccccaaccg taagctaagg tcatgcactc
    11581 tcacataacc gtctttttca agttccgcca atattatctt atggcgttgc gctatgtttc
    11641 tcataaccct ggcaccttat tactgaatcg gaattactat tcaaatgtag aaacaaaagt
    11701 aatataaaac aagataaata aaatcttaaa aacatataaa tattacgttt tattactttt
    11761 tctttctttt ttgttttgtt ttaagaaatc aaagtcataa ttttaccttg tttttctgac
    11821 aataatcata tttaagccac tataacattg gaaagagaag actttttaaa ggatttgaaa
    11881 gacaaaaagc gtcagtggga cttcattatc atcgggggag gcgctaccgg tcttggtgtg
    11941 gcggtggatg ctgtcaccag gggttacagc accctgctgc ttgaacagag tgactttgca
    12001 aaggctacat cggcccgcag taccaaactt gtgcacggag gagtgcgcta tcttcagaag
    12061 ggcgatgtgg ccctggtcag ggaagcactc cgcgagaggg gttaccttcg caggaatgcc
    12121 ccgcacctgg tgaagaacca gcgttttata atcggtaact acaaatggtg ggagaagccc
    12181 ttttacacta tcgggcttac cttctatgac ctgctctcgg gcggcatgag tctgggacgc
    12241 tccctgccca tgagccgcag cactgtggtc aagtccatcc cgcagattgt ccgcaaaggg
    12301 ctcaagggtg gtgtcgtcta tcatgacggc cagtttgacg actcccggct ggcagtcaac
    12361 ctgatgcaga cagccattga aaaaggggca accgttgtca attatacaaa ggtgatacag
    12421 ttgctaaaga cctctgaagg ccaggtttca ggagtggtgg ccgtggatga gcttgacggc
    12481 acacacttca ctgccgaggg aaaatgcatt gtcaatgcca ccggcatatt tgtcgacagt
    12541 ataatcagga tggacaaccc cgaagagaag ccgctggtga gaccgagcca gggtgttcac
    12601 cttgttgttg acaggagttt tctcggaggt gatgacgcca tcatgattcc aaagaccagt
    12661 gacggcaggg tgatgttcgg cgtgccctgg cataaccgcg ttatactcgg caccactgat
    12721 gttcccgtga aggagttcgt gcttgaaccc aaggccctcg atgaggaagt tgactttatc
    12781 ctcgagactg cgggcaggta tctcgagaga aaaccgcagc gcagtgacgt gctgtgcgtc
    12841 ttcgccggcc tccgtccgct tgcggcgcct aagcatgatg atggcggagc caaaacaaag
    12901 gagatatcgc gcagccataa gatgcttgta tcaccgtcag gtctcgttac aatcaccggc
    12961 ggcaaatgga ccacctaccg caaaatggct gaagataccg ttaacgccgg catcagggca
    13021 aggaagcttc cgttcaggcc gtgtcgcacc agggacctga agatccacgg ctggtatgaa
    13081 aatcccgaca gaacaaacag gctgtacatt tatggcagcg acctggatga actgatgctg
    13141 atgcagaagg agaatcccga atgggccgag aagcttaacg gggagtctga cttcactgcc
    13201 ggggaggtta tatgggcagt aagaaaagag atggcgcgca ccgttgatga tgtgctcgcc
    13261 cgcagggtga gggttctcta cctcgacgca aggcagtcga tatccctggc tccgaaggtt
    13321 gccgggatca tggccaggga actgggccgt gacaaggact gggtcaggca acaggtggag
    13381 gagtacacaa atctggcaaa agggtatgtg atgtcatgat ttctgacaag gatccttact
    13441 gtaacaatta attaatgtta aagaaactgg tacgaaagaa agaaactcaa tatttgcccg
    13501 cagaaaacct gataaagcaa cctgacaaac aacgcaccga tgataaactt tctgaaacca
    13561 cccccataca aggagcccct gcctgaagag aagattgaca aaacctacaa gcgtcttcgt
    13621 ctgcaggtct tcatgggagc ctttataggc tatgctgcat attacctcgt caggaagaac
    13681 ctggctctgg ccattcccgg catgattcag ccggttgaga gcggcggact gggctacacc
    13741 aagcttcagc tcggaattgc cctctcagcc atatcaattg cctatgcctt cagcaaattc
    13801 ataatgggag ctctgtcaga caggtcggat gcaagaaagt tcctggtaat cggtctcatc
    13861 ctttctgcag tgcttatgat gtcggtcggg atctttccct tcgccacgag cgcggtatgg
    13921 ataatattcc tcttcatgct tgtcataggc tggctaggcg gcatgggctg gccgccgtgc
    13981 ggccgcatca tggttcactg gttctcgcac aatgagcgaa gtttcaagat gggcatatgg
    14041 aacacctccc acactgtcgg cagcggcctg atgggtaacc tggcagcctg gggcaccatg
    14101 ctccttggcg gcctgttcct ggttgacctc ggaggaggag tgaccgtgga acagagctgg
    14161 agggcggtct tcatcttccc cagcgcagtg gcacttctgc tggcgctatt ctgctggtgg
    14221 gcactgcgtg acacacctca gtcatgcggc cttccctcca tagacaaata cagaaatgat
    14281 gtaccggtac agaaggagat gagctccgac aagaagatcc cggtgaagga gcttttcatt
    14341 aaatacatct tcaccaacag gattctgtgg ctgattgccc ttgccaacgt cttcgtatac
    14401 ctcatccgct acggcatagg cgactgggcg cccacctact gcgaggaagc aggactgctt
    14461 accagcgagc agagcaagat ggcattttca ctgcacaact atgccggcgt accgggaatg
    14521 gtgctcatcg gatggatctc ctcaaaggta ttcaagggtc gctgtgcccc ggcaaacgtg
    14581 atctatatgg tcctggtgct gctcagcgta ttgctctact ggaaagccgt acccgtggct
    14641 tcattctttg ccaatgcctt tggcacagat cccgatgcca ccagggtcac ggtagtctat
    14701 tctgccctga tagccatcgg cttcttcatc tatggcccgg tggccctcat cggcgtgcag
    14761 gccgtcaacc tcgtaccgaa gaacgctgca ggaaccgcag ccggatttgt tggcctcttc
    14821 ggatacctca taggtgacgc cctcctctca aaggtgctga taggcgcagt ggcagacaat
    14881 gtaagccttg gctggggtgc aaccttctgg atattcatcg ctgccagcat tctggccgcc
    14941 atcttctcgg caaccacctg gaaaaaggaa aaggaaacat atctcggaca cgcaaaatga
    15001 ataacatgaa acgactgata gtaactgttc ttgcagcggc attctgcctt accccgacgg
    15061 ttaatgcaca gacttaccgg catatctccg gctggtcaca ggatgttaat gaccgtatag
    15121 aagggttcct caactcaacc atcaccatga acataaggaa ggtagcggtg ttcgacagtg
    15181 acgggaccac cttcggccag gtgccttatt accttgctga cgaagcactc taccgttacg
    15241 ctgacgtggt tctgaaggag agaaaggaca gtgaggcaaa ggacaaactg aaaatcctgg
    15301 aaagaatggt aaaggacggc aacaacgtag ggaaacccta cgtggaagac agggttcatt
    15361 tcctggcagg gctgacaccc ggagagatag aaatgatagg atatgaatgc taccgggaat
    15421 catacagcag aaaaacctac ccggagatga aacagctggt tgcaaacctg aaggaatatg
    15481 gattcgaggt ctacatactt actgcatcgc ctgagttcct ctaccagaag ttcgtgtcag
    15541 aggaatatgg catccctgtc accaacgttg tgggtgccaa gtgcgtggta aaggaaggtg
    15601 tcaccaccgg cgagatcatc cctcccatcc cgcaggatga cggtaaggct cataccatag
    15661 agaccttcat caaggcaaga cccctgattg tcggaggaaa cagccgcggc gacatggaca
    15721 tgcttaacga gtcggcagga ctgaagattg ttgtcaatcc ggatgatatt accgttcgcg
    15781 gcccggagga cggtcccatg aacggccaca cggtgaaatc atactgggag aaggaaggag
    15841 cgttgatcgt caggtgcaac gatgtgcgtg acccgaaggt caggttccac actgaagagt
    15901 ggaaaatcag acagaacatt gcaaatccta aatgaccagt cacaatgaag ataagaatac
    15961 tattctcaat ggccgttacg gctgccctgc tggcaggatg cgccggcaag aacgctcccg
    16021 taacgggagg agtgcaggac tacatgcctg acaacgcggt catcgcgcat cgcggaacca
    16081 tatactgggc tcccgagctt accgagtctg ccttccgctg ggctcgcaac accggtgccg
    16141 attacatgga gctcgatgta caccgtacaa aggacagtgt acttgtaata atgcatgaca
    16201 agaccttcaa acgtactaca aacgtggcgg aggtttttcc cggcagggag ggcgatccga
    16261 taggcacctt cacatgggaa gagataatgc agcttgataa cggcagcgca ttcaacaaaa
    16321 agaaccctga ccaggcccgg cagagcttcg agggtcagaa cgtgctgacg tttgaagatg
    16381 ttttccgcat cgccgaaggc aaacggatta aaagaaatcc tgacggcagc cgtgtcttca
    16441 caaaagacga aaccggaaaa tatcactttg agtacgaggc tgacccggca gacaacggtc
    16501 acaggcccgg agtttacatt gaattcaaga tacctgatga ataccctctc attgaagaac
    16561 aggtttacga tgaactggcc aggattggct ggaatccgct tgagggagtt acaccatcag
    16621 ccgatgagcc attcctgaag ggagacaggg tcaacgtcgg caacaccagg ggaaaaatcc
    16681 ttgtgcagac attttcacag aagggaatga tgaacatcaa aagggtattt cgcgaacagg
    16741 tacccgcaag ttacctcatc ggcaatccca aaaccaacct atttgcacag gaggcagtga
    16801 ctgacagcat cgtggctttc gcgcgtgagt gcggcgcaca gtttatcggc accaatctgg
    16861 gagatgagaa tgacggcctt tctccggagt ttgcagcaaa gatccgtgcc acaggactta
    16921 agtcaaacgt ttattcattc aatacggttg agcagatgga gaaatatttc ggccagggtg
    16981 atgggaagaa agcctctcct ctcactgaag gcatgatcac caaccgcacc gacctgacga
    17041 tagatttcta tcactcccgt aaggttcgcg catacaaaac cgaaaaaagc ccgcaggaga
    17101 tacttgcgga cctggggtat aaccagtaaa atgagtaatg catgccagat ataaagaaac
    17161 tgatatttct gcttttgatt gcggcaatag ccgcctgcac cgggtgcaag gaagatccgc
    17221 ctcctccgga tgagggagga ttgaagatca cgggggtatc catccctgca agccttaaca
    17281 ttcctgccgg aggcgaggtc accattacag gcagcggatt tgcagcaggt gatcagatgg
    17341 tgttcgtgct cgtcagcgat gccggaaagg tttataccgc cacggtagca tcagtgacag
    17401 gacaaagcgc cggttttacc cttccggccg ggttaacatc cggcacgtac agcctgacgg
    17461 tcaaaagagg caccgacagc atggtgctcg gtaccctgac cataaatgtg gtagcagaca
    17521 acgccattcc ggataaaccg ggaatgaccc tcaagggggt ggtctactgt gacggagaag
    17581 ggattcccgg agtggttgtc tctgacggag cggaagttac ggttactgac agcaagggga
    17641 tctattacct gccgtcgtta aaacagaata agttcgtatt catctcacta ccaggcaatt
    17701 acgagatcgc cgcaagcgac aatattccgc agttcttcaa aaggcttgcg ggaggcaact
    17761 cggttgagca gcacgacttt tcacttgtgc atacagacaa taacaatcat gttgttctcg
    17821 ccatggccga ctggcacctg gcgaaccgca atgatgacct tgcccagttc agtaacgggt
    17881 tcctgcctga tgtgaattcc accatcagtt catacacgtc tgccgggaag aaggtttacg
    17941 gcgttccgct gggtgacatg acctgggatg cctactggta tgacaacaac ttccgccttg
    18001 aacagtatct catcgagatg aagaaagtca actgcgtgat gttcaacgtt atgggcaatc
    18061 atgacaatga tccgtatgtt cagggagacc tgccgccgga agctcctttc agggagataa
    18121 tcggtccgac ttattattca tttaacctgg ggcaggttca ttatgttgtt cttgatgaca
    18181 tccagtatat caatacaggt ggagcccagg gaaccatcgg agagcggaac tacagtgatg
    18241 ttgttgtctc cgcacagacc tcctggttgg caaaggacct tgcagccatc accgacaaga
    18301 gcacgccgct ggtgatcttc attcacgcgc aactgtacgg caaccccacc ctgaatgccg
    18361 gtggggagga ggtacacagc cttgcactga acaatggcag tacgttgctg cagatggttc
    18421 aggacttttc tgaggttcat tttgtcagtg gtcacaccca tatcaatttt accattgatg
    18481 gcggggcggt catggagcat aataccgcgg ccgtatgtgc tacctggtgg tggaccggca
    18541 aaaccggcta tgccaataac caggtatgca aggatggcag tccgggtggg tacggtatat
    18601 gggagatgaa cgggaaagac ctgaagtggc attacaagag catcggtaaa ccctcagatt
    18661 accagttcag gagctatgac ctcaacaagg tgcatattac agcggctgct tttgccccca
    18721 actctgatga cgctgcactt tcggagtatg caggagttta tgcctcccct aacccggtca
    18781 atgatgttct gatcaatgtc tggggctatg atccggaatg gaccgtagag gtcagtgagg
    18841 gtggcacacc acttactgtt gagagggtga cagcaatgga tccgctgcac ataatctctt
    18901 atgaggcaaa gaggctgaat gccggtgcaa cacccaccag ctcgtttgtg acaggtaaca
    18961 cggcacatat gttcagggta cgtgcatcat cagcaacgtc aacgctggag ataaaggtca
    19021 ccgacaggtt cgggagggtc tatactgaaa ccatgacccg gccaaaggac tttacatggt
    19081 caatgcaata aactatatat aaataactta aaactactaa tctatgaaat tcaaaccaga
    19141 tcttaaggtt attcttgcca ccttcctgat ggcactggtt atgttgccgg tgatggctca
    19201 ggagaggcag gtgacgggaa cggtctatga gcccgatggt gtcactcccg tcttcggggc
    19261 caccgtggtg ctgaagggta ccacaaccgg caccagcaca gatgcgaggg gagcctactc
    19321 gataagggtg gcaggtgcca gtccggtgct ggagatccag ttccttggat acataaaaca
    19381 ggagctcgcc gttggtgaca ggaatgtcat caacgtggtc ctccaggaag aggcaattga
    19441 agtatctgcg gttgttgtca ccgcgttagg cctgacgcgt gaagagaaat cacttggata
    19501 tgccgtttca aaggttgcca gtgaagagat cacccaggtg gtgacgaaca actggctcag
    19561 cggcatgtca ggcaaggtag ccggactcac cttcaacagc gccagttcag gcccgataag
    19621 ctcgatgagg gtgactctcc gcggcgacgc atccctgagc tatggtgcca acgaggcgct
    19681 ctttgtagtt gacggcgttc cgatcaggtc agggaccaca gccacctcat caagcagcag
    19741 ctatacaaac tccggatcgg acttcccggt tgacttcgga aacggcgcca gcgacctcaa
    19801 tcctgaagat attgaatcag tctccgtgct taaaggtcct gcagccacag ctctgtatgg
    19861 atcgcgtgca gccaacggcg caattgtaat tacaaccaaa tcaggccgta aaacgaaagg
    19921 gatcggcgta accctaaact cttcggtggt tgtggagagg ccgggttact ggcccgattt
    19981 ccagactcag ttcggatcag gatcggatat gggattgaac gagtacaact tctggaccct
    20041 cgatccgagc cttgtaggca cgactgacta tcccccgaga aacctttcgc gctatgcgtg
    20101 gggagaagag ttcagtgagg acaagctccg ctaccagtac gctggcaaga actgggagac
    20161 aggcgagatt gtcaaaaccc cgttcgtata ccaggatgac tggttcacag gcatattcca
    20221 ggacggtatt acatacaaca actctgttga catcagcggc agcaacggtg aaggacagaa
    20281 catccgcttc tcgatgactg ataccaggaa caagtggatc ctgccaaaca cgggattcca
    20341 gaaacagtcg tatgcccttt cattcgagag ccctgtcaac aagtggattg actttaagac
    20401 gaaggtcaac tattatcgca ctgacagtga caacatgccg atggccggtt atcaccagac
    20461 cacggttatg tatgacctga tgtggggtta caactccaac tcgatggccg actggaggaa
    20521 tgagtacttc aagggaagat tcaatgccat caaccgtgcg gccaccatct ccgagggcgg
    20581 caacagcctt gtatttgcag gtaccggatc atacaacccc tacagggttc tctatgagga
    20641 actgaataca accgataagg acagggtctt cggaaacctg gggctgacaa tcaagatagc
    20701 cagggggctg acacttgatc tcaggacagg tatggacttc ggcaacgagt tccgcacgca
    20761 gcgcaaaccc aagatgacct tcgggtggga gaacggtttt taccgcgaac agggcttcat
    20821 gaccttcgaa gccaacagtg acttccttct gaaatacgag agatccttcc tcgacagcag
    20881 gctgaccacc accaccgcgt ttgggggcaa caacatgagt tcgcactaca ggagcaacac
    20941 ggtgcaactt gacgagcttg acatcgaagg ggtttatcac attgacaacg tgcccttcgg
    21001 catataccag cgtccttcga gcttcagaaa ctacaagatg atcaacagcc tctatggatt
    21061 catgactctc ggctgggatg acacatatta tcttgacatc accggccgta atgactggac
    21121 cagcacactg gcgccaaaga actggtcgta cttctatcct tctgtggcat tgagcgttct
    21181 ggttgaccag atgttcgact tccgcaccac actgccggca gtatccatgg caaagcttcg
    21241 cctttcatgg gccaacgttg gtaatgatac aagtccctat ggtctcgacc agtactattc
    21301 ggcatcttcg attagcggcg gttataccct cccgtcactt atccctgacc cgatgatcga
    21361 acctgagaac atggagagct gggagctggg tcttgacacg aaattcttca agaacaggct
    21421 tggccttgat attgcgctct atcgctcttc aaccacaaac cagattatca gccttgacat
    21481 tgacccgata gtaggtgcca gcagcatgag aataaacgtt ggcgagatca ctaacaaggg
    21541 tattgagatg tcaatgtatg ccactccggt caaaaccagc gatttcacat ggaacctcaa
    21601 cttcaactgg gcggcaaaca ggaacaccct ggtcagtctg catgagagcc ttgatccctc
    21661 acagccctat gagcatgata tgggcaccac catcggcgga aggctccatc tctattcata
    21721 tgtgggccag gagatgtacc agctctacgg ctttgccctt aagagagcgc ctgaaggatc
    21781 tttctatact gataacaacg gtgaaagggt agactgctcg ggacaggtgc tgattgatgc
    21841 caccaccggc ctgccttcgg ttgagtcaac tgctgaccac ttcctgggca aggtgaatcc
    21901 tgactggaag ggtggtttcg gaacctcact caggtacagg aatctctccc tgagcgccct
    21961 cttctcatac cagatgggcg gacaccggtt ttcggtgacc catggcatac tttcatacca
    22021 gggtaagctt acgaactccc tcgaaggtcg ctatgacgga attgttgcag aaggggtgaa
    22081 tatgacaggt acaggagaag ccgctacatt tcagcctaac aacacagtca ccaacagtat
    22141 ctacacctac taccaggcta taactcttga ccgttacaac ggtgaagccc atacttttga
    22201 gacctcattc ctgaaatgga aagaagtacg ccttgaatat gccctgcctt cggtattcat
    22261 ccagaagaca agggtacttc agggagcaag cgtggcgatt tttgccacca atctgttcag
    22321 ctgggacaaa tggcctcagt tcgacccgga gggtggtatg cttaccggta ccaatgtttt
    22381 caacggcatt gaggcaggcg catatcccat gacccgtacg gtgggcgcta acctgagact
    22441 ttcattctaa tcaaaaacag gcaataaatg aaaaacatca agatattaat catctgcggg
    22501 cttgttctcg gatcactcta ctcctgcaca ggcaaattcg aggagttaaa tacaaatcca
    22561 aacaagaccc tggtggggat gatacagcct tcaggcatgt tcgaacccat actctacaac
    22621 tctgccaacc agtggcttaa ccgtacacat tattacaaca acgagcttac ccagttcacc
    22681 gccttcaccg gtggcgtgac acgcagggag catcagtatt tccttgaaga ctcaagagac
    22741 tggtacggat tctggaatca ctacgccaca tacggaaaga acattgatca catgtatgag
    22801 ctggcgatag agcacggcga caggtcactt gaggcaattg cactgacctt caaggtactc
    22861 ttcttctcaa atatgaccga catgttcgga agtatcccct acgaagaggc cttctcggcc
    22921 aggaagcctg gcggaacgct gaccccgaag ttcaacaccc agagggaggt ttacgagttc
    22981 ctttttgaag agcttgaagc ggcaaatgat atctatgcta ccaatcccgt tttcctaaat
    23041 cctgcgatgg acggaatgta cggcggtaat atggcggcat ggaggaaatt caacaactcg
    23101 ctgtatctca ggcttctctg cagggtcagc ggccgcactg agatgagtgt cggcaccagg
    23161 atgaacgcaa tacttgacaa tcccgacaag tatccggtct tcacctcaaa tgcagacaat
    23221 gcaacggtga agtttaccgg taccgatcct tacatcagca acttcgggac gaccactgaa
    23281 agcgagttca cctctagcgg aaggaagctc acccagcagt tgatcaggct tacggtccag
    23341 actgacgatc tcggaaacca ggtctatgtt gatccccgtc ttccggtcat cggcaagaag
    23401 aatcccaatg cacaggttaa ccctgcaaac gtgtggaaag gaactgtatc cggctgtact
    23461 gatattgaaa gaagtgaggt tgacagggga acctcatggc tgaatttcaa ggtgttctgc
    23521 agaagggacg ccccggccac atacatggat tatgccgagg ttgagttcat tcttgcagag
    23581 gctgcactta agggatggat cagcggaggt caggctgaag ccaaaaccca ctatgagaaa
    23641 ggagtcgatg catcaatgaa gaaatggtca gagcagggtg ccttttcaga tccggcaatc
    23701 accattacca atgcagatat tcagacctat cttgcctcac ctctcgggtc ctgggacctt
    23761 gcgaccagca aggaggagtt ccttggcaac cagaagtggc ttgcactctt ctgggtaggc
    23821 atggaagcat accatgagta ccgcaggacg gggtaccctg tcctcactat aggcgaagga
    23881 accatttaca atgacaaggt cttcccgaca cgtttcgggt atcccaacat caccatgctc
    23941 accaatgctg acaacgcggc tgatgccctc agcggaatgg gcggtgaaaa cgatatgaaa
    24001 acaccggtct ggtggagcaa acaggcaatt gccggcacga aataaccagc aaaaaagtta
    24061 agagatgaaa aacaagaaca taacaaactt cggaagaaca gcactgatag tgcttccgct
    24121 gttcctcctc tcactcttcc tcctctattc ctgcaaggaa gatccggttc aggaagatcc
    24181 gttcttcagc ctggagggga gccctacagg actgacgctt acaaaggcag ccaagaccac
    24241 aagttacgtg gttcgctcca accgtccgtg ggaggttgtg aagaagagcg gtgccgactg
    24301 gctgaaggtt ttccccgaaa agggagatgc tgacggcatc ttcaagatct atgttacgga
    24361 gaaccccctc tttgttgcac gcactgaaaa cctggcgttt attgtcgacg gaaccgaaca
    24421 accggtgttg ttccgcgttg accaggccgg caacgtgcca tatatcaccc ttcctgcaac
    24481 agtgacagta cctgccggcg gaggcccgtt ctcggttgat gtggtttcaa acgtcacgtg
    24541 gacatattcg ctcagcgacg actcatggct ggttgaaggg tcagtcaccg cccagacgct
    24601 aaacctggtt gcagaaatga atacaagcga cgatccccgg gaggtaaccc tgacactaac
    24661 atcagtagat tttcctgatg ttagtgaatc ggttgtcctt acccagtcat cgggaaaagt
    24721 tgtgcttgaa gaagacttca actggctgaa ctacgggaac gttgtattct acaccacaac
    24781 caatgaaaca cgaatagaca actggacaca ggatcagaag gacaggggct ggaccagcac
    24841 tataaataca gtgactggca gcggtaatac agcaatgctc tatgcacgcc cgggattcgt
    24901 taaagtgggc aagaccagct acggcggcga cctgatctcg cctgccttcg ctaaacttat
    24961 tggcacacag acagttacgg tgacattcaa ggcaataccc tatatgacca aagccggtgc
    25021 aatggatgaa aacgttctca aagtcagtgt catcggcccg ggtacagtaa gccaggagca
    25081 gtttatcatt gacaactggc cggtatatcc tgcagatgat ggcgcccata ctgactactg
    25141 cgtcggaatg tggagtgcac cggaagctac ccggacattc accatcaccg gagccacatc
    25201 ggaaacacag attaaatttc tgggaggaga ctatgacctg accactgagg cagggctgcc
    25261 aaccaggaag aacaggatct tccttgatga catcaaggta gaaatagtgc tgtaaaagat
    25321 tcggttagtt tcgattcaag aggtgttcat cagtgcgatg agcacctctt tttttgtttt
    25381 gatcccggtt tggcataact ttaaatgaaa ttcggaagag aaagaccatg agaaagttct
    25441 tcgtcccgcc cacacttgta tgtgtgcttg cacttgccgc atcatgttcc acaatattga
    25501 acatggagag gacctaccct cttgaggcac tcctgccggc agacagcaac agattcctgt
    25561 ttgttaattt ctatgattac caggtgcctg attttatcag ggacaggcat gaagacggat
    25621 atgccgctgc cgtaaaaggc tttgccatgg gactctcctc taccatccag acagacccgc
    25681 gcgcgttgtt catgattggt gactcgctca gaaaaggctt tacggtaata tccatgcaat
    25741 accctgaatt cagtgatact gtcagggcac tctgcagccg gtacggggcc aatctcctgg
    25801 tggctctcga ttcgataagg ttgctggttg actgggactt ctacctggct gaaaacgatg
    25861 agggaggctc catgctggcc aaggacttct accttttctc ggatacctat atgactcttt
    25921 attcagctga cggagaagtg attgacaggt gtgcaggaga aaaaaacact tttgtcaaat
    25981 cgaaatacac catcttcgga atgattggcg gtccgaatat tggcaaccag aaggagagga
    26041 taaaactgct caccggatca gccgcacagg attgcatcgg gaagtatttt cccttcaccg
    26101 aggggtacac cggcaagctt ttttccggcg gtgagctgac aaaactcaat ctcctcatca
    26161 ccgggggaaa gccggaagaa gctgtaggtc cgctgacgga actcatcaac agatcatcgg
    26221 atccaatggc atcaaaagct gagcataatc ttgcgatagc caatgaaatt attgaaaaca
    26281 ggaaagaaag ggaggagatc tggaggaaat acacaaaccc tgaaaaataa tttaattggc
    26341 aggcagaaac cccgtttagg tcgccagccg tctggtctca atggccataa tttcactaaa
    26401 aaatgcccga ttgtgcttta attcagtttt tttaatactt ttacgccgct ttcaccaata
    26461 ctgcacagta aaactcaaaa tgtgtaatta aaaaagaaat caatgaaaag gatcatttca
    26521 gttatttccg cactcgttgt cacaggctca ctctttgcag gcggccttgt caccaacacc
    26581 aaccagagtg ccctttacac caggctacag agccgcaacg cctcaacagg cattgacgcg
    26641 gtatactaca acccggcagg tcttaccaag ctgtcagatg gtttccatct ctcactcaac
    26701 aaccagacca tcggccagac gaagaccatc accaccaact acatctacct caacggcaat
    26761 cctaaagaat atgtcggcaa ggtaagtgcc ccgttcttcc ccggtatcta tgccgcttac
    26821 aagacaggca agctggcgct ttcactgggc tttaacccgg ttggcggtgg cggtggagcg
    26881 aaatatgaca acggacttgc catgttcgag atgcagatag cagacctgaa accgcttctg
    26941 acaggagcag gccttacaac tacaaattat tctgttgaga ccctttcgtt cgaaggctct
    27001 tcaatttaca tgggccccca ggttaacgtc tcttatgaaa tccatgagat gttctctttt
    27061 gctgcaggcc tcagggttgt atttgcaagt gataaatacc tgggagctat tgaaaacatt
    27121 atgatcaacc ccgtgcatcc gcttgtcaac cccacaggcg caatgatctc agcacctgct
    27181 ttctttacta ctataggaca accggcaagg gctgctcaga cttcaaatac ctatgccgat
    27241 gcagtaatga agggaaccgg tttcaccccc atcctctcag ccaacttcgc cccggtgggt
    27301 gacctcctga atattgcagt caaatacgaa ttcaaaacga agcttgatct cacaaccaca
    27361 gtcaatgaca acaagacagc cggcctcttc atccaggact ctgttgctgt caaggacatg
    27421 cccgcaatgc tgtcgttagg tgttgatgta aaaccttcgg aaaggttcct gatttcaggc
    27481 agcttaaact attatttcga caagaacgtc gattatgacg gtatgccgga tgtgaatgtt
    27541 gagatgatcg acaagaactt catcgaagcc ggactcggta tacaggttgg aataactgac
    27601 aggttctttg caagtgccgg atggcttaca actgtcacag gcgtgaatga caatttccag
    27661 aacgatctga aattcagtct caatacaaac acatttggcg gtggtatcgg cttcaggatc
    27721 aatgatatga ttgacctcaa ccttggtggt tcatatacaa tctacgagga ggcaagcgag
    27781 acttacaccc acgtgattac cacaacaccc atactggtcc aggctcctgc aatcaacacc
    27841 tacaacaaca aaacatgggt tgcagccatc gggcttgact tccatttcta ggaactgacc
    27901 tcaacctttg aaaaaaacag gggctgtctc agaaggggca gcctctcttt tttccacaat
    27961 atgaagagcc ctatcccggg gctccttttt ttatacagct cggaccttaa tgtcagtact
    28021 ctgccccctg ccgcttcaga ttctcaagat atagcttcag gtcgggcgta tgggatgtca
    28081 tcatcctgtt gtaccactgt ctggcagcct cattcctgcc catgctccgg tcaagcatta
    28141 tcagtgaagt gtatacggag ccctcaatgc caaggttaag gctgtcggtc tcgttgatga
    28201 tctcctcata aatacggtat gcagccattg aactgccagc gcctgacagc gcgaaagcct
    28261 cgttcagctt tccgttcagc ccggcatcag tcacaactcc cctgaaggag aggcatcctc
    28321 cgacacactc ctcaactatc gagaaccacg ggagaactcc cgtagcggga aaggtaagcg
    28381 aaaaagacgt tttctctccc ggccgcttaa acttgtaagt tgccggacag tacgggagtc
    28441 ccttcagccc ggtaagcttc agcctcacac cgtcaggctt cacaaggaaa gtgttcctgt
    28501 ctatgcaaaa ccacgcgttg ttgatgtcac tcattattga cagccagaca accgtggcat
    28561 cggcattgaa atcaacccgc acgatctctg ccgtcagagg cgacttcaaa ccgtaattgg
    28621 gactctcaac gctctgtccc gcggcaatat tcattaatgc cgcaagcata actgtaagga
    28681 ctatctgttt catatcagaa cctgacttgc aattatcata ccgtaaagtt aatgatttac
    28741 accaacccga ttttcagtct ttttactccc tcctgcaaac ctgcggaata ctatgtttgt
    28801 aacaactttg tcacatttgt gtaatctgaa tgtaacaaaa gcaacagatt tttgtattgt
    28861 caaaccttca attattaatc agatgagaat gaagatttta cctgtcgtta tcggcattat
    28921 gatgctgacg gcagttccat gcgccgccca ggagagtact gatttcaaac cctccggcaa
    28981 attcttcggg ttgctgtttg ccaactacca caccactttt tcaggcggtg acaacaactc
    29041 tgccttcgag gtgacgcgat catacttcgg ctatgatttc agtttttcca gggaaatttc
    29101 cagcagggtc atgtatgacg gcaccaccga ggttatcaac gggaaaacca tttattccgg
    29161 ttacctgcga aacgcct
//
LOCUS       DAOPXU010000028        28959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_1610_length_28959_cov_14.478204, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000028 DAOPXU010000000
VERSION     DAOPXU010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 28959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28959
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1610_length_28959_cov_14.478204"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="PKK03_04425"
     CDS             <1..1545
                     /locus_tag="PKK03_04425"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNX83673.1"
                     /translation="EPLPSLDLQYDYYKKILPGITVKDVNSRLQELVKDDNRFVYVQG
                     PDDREHMTEAEAMGIIAKVAEADIKPYEDITGGTDLISEELKAAEIVKTVTLPQFAAT
                     EWTLANGARVVFRHADYEKDNVSISGYAFGGSSFYPDSLEPALNLFPQVISMYGAGEF
                     DNVALTKMLAGKKASVSLGLQETTQTVSGSSTPKDFETMMQLLYLRFARPNFNKEAYD
                     AIIGRFTAVITMMQKDPNKIMSDSLSMNMTNYHPRTFLLTPESMKRIKYDDINYIYST
                     AFDDASAFTFFITGNIDEAVAREMARIYIGSLPSKYQSESYRNLEMDQPEGTVKKDIF
                     IPLTVPKATVVINYSADAKYNPAGYLSMDVLKGILDLVYTEKVREEQGGTYGVSLNAG
                     SQKRPEEKATLLIAFECDPERASELKNIIYSELDAIAKNGPRQVDLEKTVSNLLKTRE
                     EAMQHNSYWANAVSSYYLNGIDINDNRNYADILNKLTVKKMKKVTKKFLSDADLLELV
                     FLPEKK"
     gene            complement(1675..3147)
                     /locus_tag="PKK03_04430"
     CDS             complement(1675..3147)
                     /locus_tag="PKK03_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HNX83674.1"
                     /translation="MKKGTILLLAALAAVSVPLHSKDYNASMFGAKSNGTTLNTSSIQ
                     KGIDYISENGGGRLVFYVGRYLTGTIYLKSNVTIHLEEGAILVGSTNPFDYERNFNWT
                     ALIFALDQENIGITGKGVIDGQGFLVATTLVDMIHMGVVKDPLKYDRPNETIRPQNIY
                     FRGCRNIVIRDINLRDPGSWNQQYDQCRNLVIDNITVDSKSYWNNDGVDIVDCDSVVV
                     SNSYFDAADDGICLKSHSPDFVCQNVFIHNNTVRTSANGIKFGTVGHGGFRNIRIINN
                     LVFDTYRSALTFAAVDGGFVENIVVDSLRSLNTGNVIFLRIGERRPGKKGRMDGVTIS
                     NVYAEVPATKPDAGYNYEGPVEDLPRNISPSSIVGMPDAMIENIALKNIEIHYPGGGN
                     PHYAKVGLDELDKVPELGANYPEFSMFRELPAWGFYIRHARGITMENVKLICGKKDYR
                     TAVVLDDVHRATFSKLQVSEPGTRKESIYSRNSTDIVNKK"
     gene            complement(3149..6064)
                     /locus_tag="PKK03_04435"
     CDS             complement(3149..6064)
                     /locus_tag="PKK03_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNX83675.1"
                     /translation="MDRRRFISGSALAAAGWLAATRLQAMPWSPDGGRVPLPASSIPA
                     SLTFSGNGLWESFRDPSGNYRPFVRWWWNGNRVEAGELIREMHLLKAAGIGGVEINPV
                     AFPSRFDGDDLGKPSLEWPGGEWSNMLNVVFAEAKRLEMTCDLIVGSGWPFGAENTAE
                     EERSQIVVVAVRKVSGPSELLISRDEIFLEAGPAISNSWPGKKYSLLSMVLTPDPLSS
                     TDEITDLSDRTGEEFFDLFIPEGNHALYALVQVTGFMQVINGAPGASGPVLDHYNRHA
                     VSRFLNRMSDSIEPVTGPLSMHLRALFTDSMELEGSNWTGDMREEFFRRRGYDIFPFL
                     PFVLFRTGSMGNVTDFRYGVEYGPAFTEMIRRMRFDFETTKAELLEERFMQGFTGWCR
                     DLGVRSRMQAYGRGFFPLESSMAVDIPEGESWTMNWLKHRIGEEMPESDYRRGRAYTM
                     VNKYVSSAAHLTGKRVISCEEMTDTYTVFNTSLENLKVGSDQSIISGVTHSVFHGFNY
                     SPPGAPWPGWIRYGGYFNENNNWWPHFHLLNQYKARLSALLQNCDMYADIALLPPMAD
                     MWSLIGMQNEPFPSVLHAEHLSLVWEAIAKNGSGCDYISERILRDATVEGRKLCYGPR
                     KYNSLFLISVESLSPESARKLLEFVRSGGRVFCLETVPSKSTGWCNHERNDGEVRIIV
                     EKMKAYPHRFIYLEKPENDYIGWYRTVQERYRIDPWIRIDTPDPFLMQVRYSADDGSE
                     YIFMINSHLHESRATRVHFSAGLTRRHHCSVWNPETGERKRVNLDPDGSIPLDLGPAD
                     SLLFVFDREKAEEEWRPVAETGRDTHLLDKDWTAEFRHCRDGSVKEVMMDRLTDLKDL
                     PEFVHFSGTVTYRTTLECNAPDEMILNLGKVYGTSELKINGVSCGVKWYGRRIFNIEK
                     YLKTGMNTVEVVVTTSMGNYMKSLTDNPIAQYWTNAGTKNQPLQPMGMTGPVSCYKR"
     gene            complement(6057..6617)
                     /locus_tag="PKK03_04440"
     CDS             complement(6057..6617)
                     /locus_tag="PKK03_04440"
                     /inference="COORDINATES: protein motif:HMM:NF021952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HNX83676.1"
                     /translation="MMERDDILYFIALIPGKELKEKVRAVKERMKADYNAGHALKSPA
                     HITLQMPFKRNSWDEYAISAALEKFASEENPFTVRLNGFGCFAPRVIFIRIEDPNTVK
                     SVHGRLKKILAEQLGFSVSEVMNDVQPHITVATRDLTKEAFREAWPEFEKKEFRGSFE
                     VNSLFLLKHNGKNWDILKEFPFRKNG"
     gene            complement(6623..7936)
                     /gene="nhaA"
                     /locus_tag="PKK03_04445"
     CDS             complement(6623..7936)
                     /gene="nhaA"
                     /locus_tag="PKK03_04445"
                     /inference="COORDINATES: protein motif:HMM:NF018648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNX83677.1"
                     /translation="MKSIIPTAFQKFDRSQSLGGILLFGATIIALIFANSRLGDFYDT
                     LRETTLGFEAGSFSLKKPLLLWVNDGLMAIFFFMIGLEIKRELMIGELNSPSKAALPL
                     VAALGGMLVPVAFYLVLNNNPRASQGWGIPMATDIAFSLAILKLLGNRVPVGLKVFLA
                     AFAIIDDIGAVLVIALFYSSSIDWMKLVWAAIPMALLVFLNLKNLFPKYLHLACGIII
                     WYFFLKSGIHPTIAGVLLAFTVPLRQKTDAHTFTEEMCRIADEFREHDSDRTPLLTPE
                     QIERIDDIEQWTEKVQSPLQHLEHVLHGWVAMFIMPVFAFFNAGVAFRAGMDPDLPLI
                     TALALSLFLGKTIGVTLFSFAGLKLKLASLPQGVTFGQIFGTAMLAGVGFTMSMFIAS
                     LAFSGAEVLMDSAKIGIMAGSLVSGVSGYLILRFCRRENKHTYIS"
     gene            complement(7959..8357)
                     /locus_tag="PKK03_04450"
     CDS             complement(7959..8357)
                     /locus_tag="PKK03_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83678.1"
                     /translation="MKKALLLILVAITASCSQMRKGSSAMPEDDLIITRKYVGDFIEY
                     RHTGPENYDGPNIIWIKTTMEDQYGKISAYGRRCDFTGGDRLYLRRKFYSPGIVSGYW
                     VYVIENDESVSYRATDLQHDREVYIKAWFE"
     gene            complement(8510..8653)
                     /locus_tag="PKK03_04455"
     CDS             complement(8510..8653)
                     /locus_tag="PKK03_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83679.1"
                     /translation="MMNPDFFKSLDLMGKGMTAIFVVIIVIYLAVHLILKLTGGRRKE
                     PKG"
     gene            complement(8663..9799)
                     /locus_tag="PKK03_04460"
     CDS             complement(8663..9799)
                     /locus_tag="PKK03_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNX83680.1"
                     /translation="MEFLLEGITNITWQQAVMWVIGVLLIYLAIAKQLEPSLLLPMGF
                     GAILVNLPLSGALNQTLSGIGEVTGILDWMFKVGIEASEAMPLLLFIGIGAMIDFGPL
                     LSNPRLLLFGAAAQFGIFATVIASVLLGFDLRDAASIGIIGAADGPTSILVSQVLKSD
                     YIGPIAIAAYSYMALVPIIQPFAIKMCTTEKERKIRMNYNPKSVSRLTRILFPISVTI
                     IAGLVAPASIALVGFLMFGNLIRECGVLRSLSDSAQKELVNLITLLLGITIAASMQAE
                     KFVALDTVKIMGLGLVAFIFDTIAGASFAKLLNFFLKEKINPMVGAAGISAFPMSSRV
                     IQRLGLEADPTNHLLMHAAGANVSGQIASVIAGGVIIGLVPGLL"
     gene            complement(9853..11427)
                     /locus_tag="PKK03_04465"
     CDS             complement(9853..11427)
                     /locus_tag="PKK03_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNX83681.1"
                     /translation="MNKLYLTVVTIAIGLFSCQSDRATGPTDLAKNSIIPKPVSVTST
                     GGTFKLTAKSDIIVMPGSPEEVAGIGNFLAGKLNPSTGLGLGVITDGGKPGKGDIQFA
                     ISDTDPGLGEEGYELIITEKLITLTAVKPAGLFRGIQTIRQLLPPEIESDTVQPGPWI
                     IPTGTIRDWPEYGHRGEMLDVVRHFFGPEDVKRFIDHLALYKMNVLHLHLSDDQGWRI
                     EIKSWPNLTAHGGKTEVGGGEGGFFTQEEYSDIVQYAAERYITIIPEIDMPGHTNAAL
                     SSYPELNPDGKAKEPYTGINVGFSSLDTRSEITYKFVDDVVREIAALTPGQYFHIGGD
                     ESHSTKIDDYIYFVNRVQDIVESHGKSVTGWDEIAHATLKPDAVVQYWARAANAVMGI
                     NQGAKVLMSPCKYAYTDMKYDSTTVLGLSWAAYIEVDDAYNWDPATLVPEIGRENIVG
                     VQAPLWAETISTFREAEFLIFPRLPGYAEIGWTPAGMRSWDEYRVRLAAHGKRMETLG
                     IGFYRSPLVEWDQPGV"
     gene            11591..12439
                     /locus_tag="PKK03_04470"
     CDS             11591..12439
                     /locus_tag="PKK03_04470"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83682.1"
                     /translation="MKVVEPKDLEPLSFLFRGAKGQRFAEWMISFLAIDKVNRVYDNS
                     GAWSGSEFTSRLLKDVGVSYVVGNPERLRDLPGGAFITVSNHPYGGLDGIMTIDMMAR
                     IRPDYKFIVNKILSLVKTLNGNFIPVVPTGNKKTGIKAASIRGIREAIGHLGAGHPLG
                     FFPSGAVSDFTLRDMKVRDRRWQESMLHLIHSVKVPVLPIRFFDKNSAFFYFLGLINW
                     RVRLLRLPSEVFNKRGSHQRIGIGELISVEEQSRCPDPFSLGEYLRKKVYEMPVPEQF
                     VPGNLI"
     gene            12461..13177
                     /locus_tag="PKK03_04475"
     CDS             12461..13177
                     /locus_tag="PKK03_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase"
                     /protein_id="HNX83683.1"
                     /translation="MAARRPNRNNLKSLFNGEVLRNVFSDRTRTNLLKKQEEMAISWL
                     VQRIPSFITSNMLTGIGFFGNMIVCASFILASFYSRSFLLLGLIGFAISWFGDSLDGR
                     LAYYRNKPRRKYGFTLDITIDWISIILIGLGYIIYAEGAWKMLGYGFVVLYGWEMIIA
                     LIRLNITGKYSIDSGKMGPTEVRIVIGLIMIAEVLLPGSLIYSAFVVVIALLFVNVLD
                     TRYLLRIADDIDKKEHKEKT"
     gene            13209..14333
                     /locus_tag="PKK03_04480"
     CDS             13209..14333
                     /locus_tag="PKK03_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83684.1"
                     /translation="MGEITVKEVITRKDLRDFIYLPEKVHRNEPEWLPPIYMDEWELF
                     DKKKNKSFGYADGLLLTAYRDNKPVGRIMGIINRRYNEINHENHGRFCFMECYNDPDV
                     FHALISHIEQWARQNGMSKLVGPLGFSDKDPQGFQIEGFQYPLFITAANNSPFMVELI
                     EKEGYAKKIDLVNYLGDIPGEFPVVYERVLDRITKSREFEIIEFTSKKQLKPYILPIL
                     EVMNDTFAEIYGFVPLNDKEKKDFAARYLPILDPKFIKAVKKDGEVIGFAIGMPDLSA
                     GIKACRGRLIPFGIFRVLRESKRSKKLMMMLGGVKKDFRGKGIDVLMGVKILRDAINS
                     RMETIDSHLVLEDNLRMRGEYERIGCKVVKKYRIYQKELV"
     gene            complement(14408..16822)
                     /locus_tag="PKK03_04485"
     CDS             complement(14408..16822)
                     /locus_tag="PKK03_04485"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX83685.1"
                     /translation="MTATWTVSYTGVDDGGAPVTIRFDWNNGNIETLSATETSPGVFE
                     ATSINTYTSAGSICNYHPQATLVVDGVLCTSSSQEQIVTVWDDDDHNGGHMHINPLVY
                     PICFGNSANVRFQDLTQFNCVPPQERDNPNVSTRWIQWVYGTDITMTGIPVTVDGHSR
                     TFPYVDNVITLTGPVTGSGVWSDVLFVANDKLIGQYFEVTLRNWNYCNPYDDPAIPGG
                     PHDRVNGDHPPVVTTARILIVPYPDATIMPVDPMCANASPVALTGHDPGGTWTGSGVS
                     GNIFDPSLAGPGSHTIRHEIVNADGCRDDDQTTITVYPVPDATIITAGIACSTDPVIV
                     LQANDPGGVWSGTGVSGNLFYPAVAGSGNHLITYSITDANGCTDTDSAILTVATPDAT
                     ITPLDTLCEDSPSVLLTAHDLGGVWSGPGVVGNRFVPALAGTGNHIIKYEILNTDCAA
                     VDTAVVTVMALPSIIIDDPGTLFLNGPPVALTAYPTGGTWSGPGMTGNLFDPSEAGLG
                     NHMLTYSIPPESWGCAAQRDVYVSVIMPPMPVADFEGETSGCAPLTVQFINKSQHGES
                     YTWDFGDRQYSSEKDPVHTYFVPGTYLVKLTVHNIAGESVRNGVIEVYQNPSAIFDAY
                     PKDVVNNEQIVIFYSYSYFAESWLWRFGDGETSAEENPWHKYESPGSYTVSLLVSTHE
                     GCVDSAEMDTPVVVEWKEGTLKFPNVFRWNRTGPTGGEWSEGVNPSMDEVFRPFFENI
                     IEYRLQIFNRWGVLIYESDNLKKGWDGYFGNGNLAPQGVYVWKASGRYADGEYFDMVG
                     DVTFLH"
     gene            17241..18770
                     /locus_tag="PKK03_04490"
     CDS             17241..18770
                     /locus_tag="PKK03_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HNX83686.1"
                     /translation="MNGILTGRLSGTVKHIFHYLLVILIIGLTTLVCLPLSSPQSYHI
                     VSFILLFVVAILAVFLGIGPVLLASTMSAVSWNYFFIPPFHTFHIANTEDRLMFVSFF
                     IIALLNGILTNRIRRQERAVRDRENQTNALFRLTAGLSGARGTDEVIRVAEEHFRTNS
                     GAGARFIIQDGNNNLVCQPAAESGEDDDQPDHDLVKWVFANQRKAGRFTGNRPHEPFT
                     YFPLTGAMINPGVAVCRIAEERYRNKDAFWDTCCSHIAGAIEREFLGEMATRARVLDE
                     SDRFYGTLFNLVSHEFRIPIAAIMGASDTLMSSEASEENRKELYGEILKASARLNHMT
                     ENLLNMSRIESGRISLRLDWHDVTDLFQRVTLSLKQELAQFHVIMSIPEDMPLVRIDF
                     SLVELILNNLLLNATQHSPPGSAIKTEASYENKGLVIRVSDSGPGFPPASLPRLFDKF
                     FRVEGSPAGGLGLGLSIVRGLVDAHRGTVSVSNIREGGAIFTVTIPSEIPDINQLNLA
                     E"
     gene            18767..19450
                     /locus_tag="PKK03_04495"
     CDS             18767..19450
                     /locus_tag="PKK03_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX83687.1"
                     /translation="MNDNRTILIIDDEVQIRRLLEISLSTYGFNTVFATTGKEGLVAA
                     ATHSPALVILDLGLPDMDGMKLLETLREWYTKPVIILSVRNSEDDIIKALDRGANDYL
                     TKPFRGGELVARIRAAIRLSEEKKDTPLMEFGSLTIDMLNHVVRKSGEIIKLTSTEYS
                     LLTLLAKNTGRVLTHRFILKEIWGYGYLEQTQYLRVFIAQLRKKIEENPSNPVLLITE
                     SGIGYRFGS"
     gene            19607..21049
                     /locus_tag="PKK03_04500"
     CDS             19607..21049
                     /locus_tag="PKK03_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNX83688.1"
                     /translation="MLINPVHIARVLGTILLIEAAAVIFCLPVSLIYSEPVWPFVISA
                     GICIILGGALWFLGRRPDQGQINNRETYLIVTSAWLVISFAGALPYLIGKTIPSFVDA
                     LFESASGLTTTGASILTDVEALPHSILFWRSMTHWIGGIGIIVLVILVLPSLKMTGYQ
                     LFSLESSLKEKIHPKAKGVVYRILYIYLGITALETIFLLLGGMDLFNSLCNSFSTVAT
                     GGFSTRNTSVAEYSPYIQYVIAIFMLLSAVSYVVFYHLLKGNLKRVRSNDELWAYLFT
                     VTAGVTVLTIILYTGTDRNFALSFRHSFFQVISQISCSGFATADYMTFPPLGWFLLFL
                     LMFSGGSTGSTTGGIKIARHLIFFKNLGNSFIRLHHPSAVVPVKLNGQGVPEGVVNQT
                     MVFISLYFVIYIAGVLLLQLSGVSILESAGAVATSMANIGPGLGHSGNMGNFAHFNPF
                     AKTIMTLLMIAGRLELFTFLTVFTRPFRKN"
     gene            21112..22833
                     /locus_tag="PKK03_04505"
     CDS             21112..22833
                     /locus_tag="PKK03_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HNX83689.1"
                     /translation="MKITVNDTLLDLHNGARVRDAIAGFYKHEGRKVPERLPVAEDRY
                     GNIVAPDGALTDGNALFILSGRKKRTRLIRLLVVILAAGLLFACSSTRHAATSPDMKK
                     QGSTTVAAPAGTVAPDDRQAVIFSVNDMHAAIDNFPKLAWIVDSLRSVYPNLLLIAAG
                     DNQTGNPINDQFPEKGLPMIDLMNAVGFNLSAVGNHEFDTGPEGFSNLILKANFGFIC
                     ANVSGGGRHSLKISPYKIFTLPNGLKVAFLGLLQLGQNGIPDSHPDNVRDFVFRSPCE
                     TALEYLWLKDSSDVFIALTHIGFEEDVKLAETMPAGIDLIIGGHSHTRVDREQIHNGI
                     LITQAENKLKYGTLIRLTVNTDGRITRSMQLIDIRNSKKEKPEIRSMVDRYNDNPVLN
                     QVIATATDDFSSYEELGYLMADAQRAAAGADIALMNPGGVRIDQLPKGPVTIKNVYQL
                     DPFGNELVVTKLTGSEIFSLLRAAWPVDEKSPVYTSGITTGTRVNDQGDPEEIKILTN
                     DGKPLDMNQVYTVAMNSYMTQVYRYEHADPGQSLFFTTAEALIRYLKQEGNLRSYRGE
                     KRVRVIR"
     gene            22978..23916
                     /locus_tag="PKK03_04510"
     CDS             22978..23916
                     /locus_tag="PKK03_04510"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNX83690.1"
                     /translation="MLICIVIMTVMVQCSKSPAEGRWTTFSAEDGLANNWITAITVDS
                     RGNKWFGCMIRSISCYNNRSWTTYSVPEEFVCGYIFDIAVDKDNNKWIGTLGSGAIRF
                     DGVNWTSYNESNGFPGNDALAVATDSDGNVWFGTRGGVVRYDGEQWVTFTDGDGLVSD
                     TITAMAEDRDGNMWFGSVSGDISRFDGTSWENFHRPADPMKHIINDIAADQDGNIWVA
                     TVFGVLRYSGTGWRVFSKHDGLCSYGAIAAEVDKNGRRWFATDDGVSSFDGINWTCYT
                     GDNGLASNGVTSIAVEPDGTLWFGTGEAGVSRLSFE"
     gene            23935..24600
                     /locus_tag="PKK03_04515"
     CDS             23935..24600
                     /locus_tag="PKK03_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNX83691.1"
                     /translation="MQDKDYIVFDFDGTIADTLELGLNVYNRIAPEYNCQPVGEGERE
                     LFRTKKPQELLEAYGISKLKLLTLTLRIRKELNRHVPEMKLFDNMEKALHEIRDAGFG
                     MGILTSNSVENVRRFLEINNLTALFDFIYSGRSFLGKDRVIRKMIIREQLPAGRIVYA
                     GDETRDIEACRAAGIPVIAVSWGLNRRELLASLFPDQIADRPEELPDRLRQILRFKTE
                     NRS"
     gene            complement(24837..25157)
                     /locus_tag="PKK03_04520"
     CDS             complement(24837..25157)
                     /locus_tag="PKK03_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HNX83692.1"
                     /translation="MNIESLREYCISKKDVTESFPFGDDTLVFKTQGRIFALANLEGD
                     LSVNLKCDPGLAIELRERYSSVMPGYHMNKKHWNTVRIDGSVPDREVFSWVDNSYDLV
                     SKKK"
     gene            complement(25204..25950)
                     /locus_tag="PKK03_04525"
     CDS             complement(25204..25950)
                     /locus_tag="PKK03_04525"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HNX83693.1"
                     /translation="MQIGQMDTSDRQWRLIFKTGAIASIIVLCGIVTDMIVGSVTGGN
                     VAELPQTSVERFNQFNENRLLGLYNLDLLNIINQIILIPVIIALYGAHRNTNSSFALL
                     SMVLFLVGTTIFVTGNTALTMLDLSRKYFAAGSDEQRLLLAAAGEAMLTKGSHGSLGV
                     FIGFALPTFANMLMSFVMLSGKIFSRLSSYFGIIGNSLMIIYLVLVTFIPKVEAMAIA
                     FAMPAGLMVMAWMILFTIKLFRLSKVGEAN"
     gene            26582..26926
                     /locus_tag="PKK03_04530"
     CDS             26582..26926
                     /locus_tag="PKK03_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83694.1"
                     /translation="MVYYAPKSKNVVTSLYHELTDDHKAFFNTYSDNEEDARLFFAVF
                     FNGFYIAHELGHGLVAAYSLSDPEAMYREEYDVNMIAMNYWNSVGKADELEKCYKFAK
                     AFLAKVPDPVPQ"
     gene            27337..28290
                     /locus_tag="PKK03_04535"
     CDS             27337..28290
                     /locus_tag="PKK03_04535"
                     /inference="COORDINATES: protein motif:HMM:NF021537.2"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HNX83695.1"
                     /translation="MKIYRPFLSLTLVGLAVIGCTNKVPDFERAAFTINNQRIEVLTT
                     FDLIDNYLSHSGKFNGNIVKSSDKYIYKPIRDEILDDAEAAFLFNTIKVPYETNDYLR
                     NEIQLLKSSDLLTIIQESFTKITKELPGPDTKIIILPASSLNRPALEKIKLPISGITL
                     GSGKIILSIDPTFQNWTDFLPYCIAHEYHHSTWCSRNWISADFSLIEYLVFEGRADLF
                     ASEQYKTVSNPLTNFLTREQETEVWELIKNEIFGKGHDRINGVMFGTDKIPFGSGYNI
                     GFNIVRLFKERNPATNTLDLMDMNPNEIFERSGYEDYKAQK"
     gene            28674..>28959
                     /locus_tag="PKK03_04540"
     CDS             28674..>28959
                     /locus_tag="PKK03_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83696.1"
                     /translation="MNKLALIASMTAFLLCGCKEVPDNSEDDLTIELEGWEILKSTYD
                     FDINPRDMYFVDESIGFVVGYNGDIYKTTDSGTTWRKLNSGTTLHLHSVYF"
BASE COUNT         7542 a         7381 c         6893 g         7143 t
ORIGIN      
        1 gaaccccttc cttcgctcga tctccagtat gattactaca agaaaatcct gcccggtatt
       61 accgtgaaag atgtgaactc caggctgcag gagcttgtga aggatgacaa caggttcgtc
      121 tatgtccagg gacccgatga cagggagcac atgacagagg ccgaggccat gggcataata
      181 gcaaaggtgg ctgaagccga catcaaaccc tatgaggata tcacaggagg aacagacctt
      241 atatctgaag aactgaaagc ggctgaaata gtcaaaacag tcactctgcc tcagttcgca
      301 gctaccgaat ggaccctggc gaacggagcc agggtggtgt tcaggcatgc cgactatgaa
      361 aaagacaatg tcagcatcag cggatacgct tttggcggat catctttcta ccctgattca
      421 cttgaacctg ccctgaacct gttcccgcag gtgatctcaa tgtacggagc cggagaattt
      481 gacaacgtgg ccctaacaaa aatgctggcc ggcaagaagg cgtccgtttc actcggcctc
      541 caggaaacca cacagacagt cagcggctct tccacaccaa aggatttcga gacgatgatg
      601 caattgctct accttcgctt cgcccgcccc aacttcaaca aggaggcata tgacgctatc
      661 atcggcagat tcacggcagt aatcacaatg atgcagaaag atccaaacaa gatcatgtct
      721 gacagcctct cgatgaacat gaccaactac cacccgagga cattccttct gactccggaa
      781 tccatgaaac gcattaaata tgacgacata aattatatct acagtacggc ttttgacgat
      841 gcttcggcat ttacattctt catcaccggt aacatcgatg aggctgtagc ccgggaaatg
      901 gcccgtatct atatcggatc actcccctca aaataccagt ctgaatcata ccggaacctg
      961 gaaatggacc agccggaagg aaccgtcaaa aaagatattt ttatcccgct gaccgtgccg
     1021 aaggcaacag tcgtgataaa ctattcagca gatgctaaat acaatccagc cggttacctt
     1081 tccatggatg tactgaaagg catacttgac ctggtctata cagaaaaagt cagggaagaa
     1141 cagggaggca cctacggcgt cagcctgaat gccggatctc agaaaagacc ggaggaaaaa
     1201 gcaacattgc tgattgcatt tgaatgtgat cctgaaagag cttccgaact caagaatata
     1261 atctattcgg agcttgacgc gattgccaaa aacggcccga ggcaggttga ccttgaaaaa
     1321 actgtaagca atcttctcaa gacccgcgag gaggctatgc aacacaacag ctactgggca
     1381 aacgccgtca gctcatacta ccttaacggg attgacatca atgacaaccg caactatgct
     1441 gacattctta acaaacttac agttaagaag atgaagaagg ttacaaagaa attcctgtct
     1501 gatgcagatc ttctcgagct ggtattcctt ccggagaaga aataaaatca ccaatcacac
     1561 ggcctgtctc tgggccgtgt gtttttttat tgtcttccat ttcgataatt tttacccccg
     1621 gtatagtcaa ccgggttatc cggcattctg ttcactggat tcagatatag ctccctattt
     1681 tttatttacg atatcggttg aattgcggga atagatgctt tccttccggg ttccgggttc
     1741 cgaaacctgg agtttactga atgtcgctct gtgtacgtca tcaagcacca ccgcggtccg
     1801 gtaatccttc tttccgcata tcagcttcac attctccatt gtgatccccc tggcatgcct
     1861 gatgtagaaa ccccatgccg gtaactccct gaacattgaa aattccgggt agttcgcccc
     1921 gagttcaggc accttgtcaa gttcatcaag acctactttg gcgtagtgcg gattgcctcc
     1981 gccgggataa tgtatctcga tattcttcag ggcaatgttc tctatcatgg catcaggcat
     2041 accgacaatg gatgaaggag aaatattcct cggaagatcc tccaccggac cctcgtagtt
     2101 gtacccggca tcaggtttgg tagccggaac ttcagcatag acgttggata tggtcacccc
     2161 gtccatccgc ccttttttgc cgggacgccg ttcgcctatc ctcaggaaga tgacattacc
     2221 ggtgttcagt gaccgcaggc tgtcgacaac aatgttctcg acaaatccgc cgtcaaccgc
     2281 cgcaaatgtc agagctgacc tgtaggtgtc aaacaccagg ttgttgatta tccgtatatt
     2341 ccgaaaaccg ccatggccaa ccgtgccgaa cttgatcccg ttggcactgg ttcttacagt
     2401 attgttatgg atgaacacat tctggcatac gaaatccgga ctgtgagatt tcaggcagat
     2461 gccgtcatcc gcagcatcaa agtatgaatt ggagaccacc acgctgtcgc aatccacaat
     2521 gtctactccg tcattgttcc agtaactctt gctgtcgacg gtaatattgt ctatcaccag
     2581 gttgcggcac tggtcgtatt gctggttcca gctgcccgga tccctgaggt tgatgtccct
     2641 gataacaatg ttccggcagc ccctgaaata aatattctgc gggcgtatgg tctcattggg
     2701 acggtcatat ttcagcggat cctttaccac acccatgtgg atcatgtcaa caagggtggt
     2761 ggcaaccagg aatccctggc cgtcaataac ccccttgccg gttattccga tgttttcctg
     2821 gtcaagtgca aagatcaggg cagtccagtt gaagttcctc tcgtaatcaa agggattcgt
     2881 cgaacccaca aggatggctc cctcctcaag gtggatggtc acatttgact tgagataaat
     2941 cgttcctgtc aggtaccgcc ctacatagaa gaccaggcgg ccgccgccat tttcacttat
     3001 atagtcaatc cccttctgga ttgaactggt gttgagtgtc gtcccgtttg actttgcgcc
     3061 aaacatcgat gcgttgtagt ccttcgaatg aagcggaacc gagacagccg ccagggctgc
     3121 cagcaataag atcgtgcctt ttttcatatc atcttttata gcaactgaca ggtccagtca
     3181 ttcccatcgg ctgcaggggc tggttttttg tcccggcatt cgtccagtac tgtgcaatgg
     3241 gattgtcagt cagtgatttc atgtaatttc ccatggaggt ggtgaccacc acctccactg
     3301 tgttcatccc cgtcttcagg tatttttcga tgttgaagat cctccggccg taccatttta
     3361 caccgcagct tactccgttt atcttcagct cagaagttcc gtacaccttt ccgaggttga
     3421 gaatcatctc atccggagca ttgcattcca gagtggtacg gtatgtcacc gtcccggaaa
     3481 aatgtacgaa ctcaggcaaa tccttcaggt ctgtcagccg gtccatcatc acctccttga
     3541 ccgagccgtc gcggcagtgt ctgaattcag cagtccagtc tttatcgagc agatgcgtat
     3601 cccttccggt ctcagctacc ggcctccact cctcctctgc tttctcccgg tcaaagacaa
     3661 aaagaagcga atctgccggg ccgagatcga gcgggatgct gccatccgga tcaaggttca
     3721 cccgttttct ctctcccgtc tccgggttcc atacggagca gtggtgcctg cgggtcagcc
     3781 cggcggagaa atgcacccgt gtggcgcgtg attcatgaag atgcgaattg atcataaaaa
     3841 tgtactcaga tccgtcatca gcactgtatc ttacctgcat aaggaaagga tccggtgtgt
     3901 caatcctgat ccatggatca atccggtacc gctcctgaac agtcctgtac catcctatat
     3961 agtcattttc aggcttttca agataaatga accgatgcgg atatgctttc atcttttcaa
     4021 caatgatcct cacttcacca tcatttcttt catggttgca ccacccggtt gatttcgaag
     4081 gcaccgtttc caggcagaat accctgcctc ccgatctcac aaactccagc agcttgcgtg
     4141 cgctctcagg agagaggctc tccacgctga tcaggaataa tgaattgtac ttccggggac
     4201 cgtagcagag ctttcttccc tcaaccgtgg catcccggag gattctttcc gagatataat
     4261 cgcaaccgct gccgttttta gctatcgcct cccataccag tgagagatgt tcggcatgca
     4321 gcaccgaagg gaacggctca ttctgcatcc cgatgaggct ccacatgtcc gccattggcg
     4381 gcaacaatgc aatatcggca tacatgtcac agttctgcag cagcgccgac agcctggcct
     4441 tgtactgatt caggaggtga aaatgaggcc accagttgtt gttctcattg aaatagcctc
     4501 cgtaccttat ccatcccggc cagggagccc cgggcggtga gtaattgaat ccgtggaata
     4561 ccgagtgcgt caccccggaa ataatgctct ggtcactgcc aaccttaagg ttttcaagag
     4621 aggtgttgaa gacagtatac gtgtcggtca tctcctcgca gctgatcacc cttttgccgg
     4681 tcaggtgagc agcggatgaa acgtacttgt tgaccattgt gtaggccctg ccgcgacggt
     4741 agtcactttc cggcatctct tcccctatcc tgtgcttcag ccagttcatg gtccatgact
     4801 ccccttcggg aatatccacg gccatgctgc tctccagagg gaaaaagcct cttccgtaag
     4861 cctgcatgcg tgatctgacc cccaggtctc tgcaccatcc ggtaaagccc tgcatgaacc
     4921 gctcctcaag aagctccgcc ttcgtcgtct caaaatcaaa ccgcatgcgg cggatcatct
     4981 ccgtgaaagc aggaccgtac tccactccgt acctgaaatc tgtgacattg cccatggagc
     5041 ccgtcctgaa cagtacaaat ggcaggaagg ggaagatgtc ataacccctc cttctgaaaa
     5101 actcctccct catgtcaccg gtccagttgg aaccctcgag ttccatgctg tcagtgaaga
     5161 gggcacggag atgcattgaa agaggcccgg tcacaggctc aatggagtct gacatcctgt
     5221 tgaggaaccg gctgacagca tgtctgttgt agtggtcgag caccgggccg cttgcacccg
     5281 gagctccgtt gatcacctgc atgaaaccgg tgacctgcac cagcgcatag agcgcatgat
     5341 tcccctcagg tatgaaaaga tcgaagaact cctcaccagt cctgtctgaa agatctgtta
     5401 tctcatccgt tgaactgagc gggtcaggcg taagcaccat agaaagcaga gaatactttt
     5461 taccaggcca cgaattactt atggcagggc cggcctcgag aaagatctca tcccgtgata
     5521 tcaataactc tgacggtccg ctgaccttcc ggacggcgac aacaactatc tgtgacctct
     5581 cctcttcagc tgtattctct gcaccgaaag gccatccgga accgacaatc agatcgcagg
     5641 tcatctcaag ccgtttcgcc tcggcgaata caacattcag catattgctc cactccccgc
     5701 ccggccactc cagtgaaggc ttcccaagat cgtcgccgtc aaaacgggat gggaatgcca
     5761 cagggtttat ctcaacacct cctattcctg ccgccttcag aaggtgcatt tccctgatga
     5821 gctcccctgc ctcgaccctg ttaccattcc accaccatct gacgaaggga cgataattgc
     5881 cggaaggatc gcggaatgac tcccatagcc cgttgccgga aaatgtcagg gatgccggga
     5941 tggaggatgc cggaagggga acgcggcctc cgtcaggtga ccagggcatc gcctgcagac
     6001 gtgttgccgc aagccatccg gcagcagcga gggcgcttcc tgagataaac ctccttctat
     6061 ccattttttc tgaatggaaa ctctttaagt atatcccagt tttttccgtt atgcttcagc
     6121 agaaagagac tgttaacttc aaatgaaccc ctgaattcct ttttctcaaa ttcaggccac
     6181 gcttccctga atgcttcttt cgtaagatca cgggtggcta ctgttatgtg tggctggaca
     6241 tcattcatta cctcgctgac agaaaatcca agctgttctg ccaggatttt ttttaaccgg
     6301 ccatgtactg actttaccgt attcggatcc tctatcctga taaaaatgac cctcggggcg
     6361 aagcagccaa agccattcag cctgacagta aaaggattct cctccgaggc aaatttttca
     6421 agggcagcag agattgcata ttcatcccag ctgttccttt tgaaaggcat ctgcagtgta
     6481 atatgagcag gcgatttgag tgcatgaccg gcgttatagt cagctttcat tctctccttg
     6541 acagcacgca ccttctcctt taactccttc cccggaatga gggcaatgaa gtacaggata
     6601 tcgtctctct ccatcatcct gctcagctga tgtaagtatg tttattttcc ctgcggcaga
     6661 acctgaggat caggtatccg gaaacgccgg aaacaagtga cccggccata atgccaatct
     6721 ttgcagaatc catcagcact tcagcgccgg aaaatgccag gctggcaatg aacattgaca
     6781 tggtgaaccc gactcccgca agcattgccg taccgaagat ctgtccaaag gtgacaccct
     6841 gaggcaggct ggccagcttt agcttcaggc cggcgaagga gaagagagtc acccctattg
     6901 ttttgccaag gaagagactg agagcaaggg ccgtgatcag cgggagatcc ggatccattc
     6961 cggcacggaa ggcaaccccg gcattgaaaa aggcaaacac gggcatgatg aacatggcta
     7021 cccatccgtg aagcacatgc tcgagatgct gaaggggaga ctgcactttt tcagtccatt
     7081 gctctatgtc atcaatcctc tctatctgtt caggagtgag aagaggtgtc cggtcagaat
     7141 catgctccct gaattcatcg gcaatcctgc acatctcttc tgtaaaggta tgagcatcag
     7201 tcttctgcct gagcggaacc gtaaaggcca gcagtacccc ggcaatcgtg ggatgaattc
     7261 ccgacttgag aaagaaatac cagataatga tgccgcatgc aaggtgaagg tacttgggaa
     7321 agagattttt caggttcagg aagacaagca gggccatggg tatagcagcc catacaagct
     7381 tcatccagtc aattgatgaa ctgtagaaaa gggcaatgac cagcacggcg cctatgtcat
     7441 caattattgc aaatgcggcc agaaaaacct tcagtccgac aggtaccctg ttacccagca
     7501 gttttaatat agccagggaa aaagcaatgt cagtggccat cggtattccc cacccctgtg
     7561 atgcccgggg attattgttc aggacgagat aaaaggctac cggaacgagc atacctccaa
     7621 gggcggcaac aagaggcagg gctgccttcg agggcgaatt cagctccccg atcatcagct
     7681 ccctcttgat ctccaggcct atcatgaaaa agaaaatggc cataagacca tcattgaccc
     7741 ataacagcaa tggttttttc aggctgaagc tccctgcttc gaaaccaagc gtagtctccc
     7801 tgagcgtgtc gtagaaatcg cccagccggg agtttgcaaa tatcagtgct atgattgttg
     7861 caccaaaaag cagtatcccg ccaagactct gtgaccggtc aaatttctgg aatgcggtag
     7921 gaataatgct cttcatctgc aacaggctgc ggaataagtc attcaaacca ggctttgatg
     7981 taaacctcac ggtcatgctg aagatcagtg gcacggtaag aaaccgactc atcattttct
     8041 atcacataga cccaataacc ggaaacaatt ccgggactgt agaatttcct tctcagataa
     8101 agtctgtcac caccggtaaa gtcgcatctg cgtccgtagg ccgatatttt cccatactgg
     8161 tcctccatgg tggttttaat ccagataata ttcggaccgt catagttctc cggccccgtg
     8221 tgacggtact cgataaaatc gccgacatat ttcctggtga tgatcaggtc atcctccggc
     8281 attgcggaag agcctttcct catctggctg catgatgccg tgatggccac aagaatcagt
     8341 aaaagcgctt tcttcatccg gtcactgatt ggtccgacat atgccgcagg ccccctgacg
     8401 ggggaaacgg cctcataaaa ttactaaatt tttctgacca atccgcctgt attaattgca
     8461 ggcgctcaga cagggagcca cagacgcccc gaaaagtgcc ccgggaagcc tatcctttcg
     8521 gctccttcct cctgcctcct gtgagcttca ggataaggtg aacggccagg taaattacaa
     8581 taatgacgac gaagatggct gtcatgccct tccccatcag gtcaagcgac ttaaagaaat
     8641 ctggattcat cattcacctc ctttacagta atccgggtac aaggcctatt atcacaccgc
     8701 cggcaatgac gctggcaatc tggcccgaaa cattggcacc cgccgcgtgc atcagcaggt
     8761 ggtttgtagg atcagcctcc agtcccagtc tctggatgac ccttgaagac atggggaatg
     8821 ctgaaatacc tgcagcgcca accatggggt taatcttttc cttgaggaag aaattcagaa
     8881 gcttggcaaa tgaggctccg gcaattgtat cgaagatgaa tgccaccaga cccaggccca
     8941 taatcttgac agtgtcaagc gccacgaatt tttcagcctg catgcttgcc gcaattgtta
     9001 ttccaagcag cagggtaatg aggttcacca gctccttctg agcgctgtca gacaatgacc
     9061 tgagcacacc gcactccctt atcaggttgc caaacatcag gaagccaact agagcaattg
     9121 aagccggagc caccaaacct gcaattatgg taactgatat cgggaacagt atccttgtca
     9181 gccgtgaaac actcttcgga ttataattca tccttatctt ccgctctttc tcagtagtgc
     9241 acatcttgat ggcaaagggc tgtattatgg gaaccagggc catgtaggaa tatgccgcaa
     9301 tggcaatcgg cccgatataa tcactcttaa gcacctgtga taccaggatg gatgtaggac
     9361 catccgctgc cccgataatc ccgatagagg cggcatccct gaggtcaaaa cccagcagaa
     9421 cggatgcaat cacggttgcg aatatcccga actgcgctgc cgcgccgaaa agcagaagcc
     9481 tcggattgga gagcagcgga ccgaagtcta tcatggcccc tatcccgata aacaacaaca
     9541 gcggcatcgc ttctgacgcc tcaataccta ccttgaacat ccagtccagg atgccggtca
     9601 cctctcctat ccctgacagg gtctggttaa gcgcccctga caacggaagg ttgacaagga
     9661 tggcaccaaa tcccattggc aaaagcagcg acggttcaag ctgtttcgcg attgcaaggt
     9721 atatcagcag caccccgata acccacatca cagcctgctg ccatgtgata ttggtgatac
     9781 cttcaagtaa aaattccatg atcttttata gtgatttgtt tacagtcagt ttttacccgg
     9841 cattatttgt ttctacacgc ccggctgatc ccattcaacc agcggggagc gataaaatcc
     9901 gattccgagt gtctccatcc gtttcccgtg tgcggccaga cgtacccggt attcatccca
     9961 tgaccgcatt ccggcgggag tccagcctat ctctgcatat cccggaaggc gcgggaaaat
    10021 aagaaattcc gcttccctga aggttgagat ggtctctgcc catagcggcg cctgtacccc
    10081 gactatattt tccctgccga tctccggcac aagtgtcgca ggatcccagt tgtaagcatc
    10141 atccacctct atgtatgccg cccagctcag ccccaggacc gtggttgagt catatttcat
    10201 gtcagtatag gcatacttgc agggtgacat gagcactttg gcaccctggt taattcccat
    10261 caccgcgttt gccgcccgcg cccagtactg gaccacagca tcgggcttta gcgttgcatg
    10321 tgcaatctca tcccagcctg ttacgctctt accatgtgat tcaacaatat cctgaaccct
    10381 gtttacaaag tagatgtaat catcgatttt cgtagaatgc gactcatcgc ccccaatgtg
    10441 aaagtactgc cccggtgtaa gagccgcgat ctcgcggaca acatcgtcca caaacttata
    10501 agtgatctcc gaacgggtgt caagtgagct gaacccgaca tttattcctg tatacggctc
    10561 tttggcttta ccgtcggggt tgagctccgg gtaggatgag agggccgcat tcgtatgacc
    10621 aggcatgtct atctcgggta ttatcgttat gtacctctcc gcagcatact gcacaatgtc
    10681 tgaatactcc tcctgggtga aaaaacctcc ttcgccaccg ccgacttccg tcttcccgcc
    10741 gtgagcggtc aggttgggcc atgatttgat ctctatgcgc catccctggt catcagaaag
    10801 atgaaggtga agaacattca ttttataaag tgcaagatga tcgataaacc tctttacatc
    10861 ctcagggcca aaaaaatggc gaacaacgtc aagcatctct ccgcgatgcc cgtattccgg
    10921 ccagtcacgt atggttcccg tgggtatgat ccatggcccc ggctgaaccg tatctgattc
    10981 aatctcaggc ggaagaagct gcctgatagt ctggatgccg cggaagagcc cggcaggctt
    11041 gacagcagta agggttataa gcttctccgt aatgataagc tcatagccct cctcgcccag
    11101 gcccggatca gtgtcgctga tggcaaactg tatgtctccc ttcccgggtt tgccgccatc
    11161 agttatcacc ccgagcccga ggccggtgga aggattcagc ttgccggcaa gaaaatttcc
    11221 aatgccagcc acctcttccg gactgcccgg catcacaatg atatcactct tggcagtaag
    11281 cttgaatgtc ccgccggtgg aagtcaccga taccggtttt ggaattatac tgttctttgc
    11341 gagatcagtc ggacctgttg ctctgtcact ctgacaggag aacagaccta tggcaattgt
    11401 aaccacggtc aggtaaagct tgttcatcgg cttaggtttt acggatgttt caaattctgc
    11461 ggaaaactca gttccaaata tattgttttt gaaacacgaa ttccatttca ttcctcatcc
    11521 cccataacct ggaatgaaat aatcctccat ggtctgatga tttttaattt gtaaatttgg
    11581 gatatacgcc atgaaggtag ttgagccaaa agatctcgaa ccgttgtcat tcctgttcag
    11641 aggcgctaaa gggcagcgct ttgcggagtg gatgatcagc tttctggcaa tcgacaaagt
    11701 caacagggta tatgacaatt caggagcttg gagcgggtcc gaattcacct ccaggcttct
    11761 taaagatgta ggagtcagct acgtggttgg caaccccgaa aggctgagag accttcccgg
    11821 aggcgccttc attaccgtat caaaccatcc gtatggcgga ctcgacggga ttatgacaat
    11881 cgacatgatg gccaggataa gacccgatta caaattcatt gtcaacaaaa tcctctcact
    11941 ggtaaaaacc ctcaacggca actttatccc tgtggttccg acaggcaata agaaaaccgg
    12001 gatcaaggct gccagcatca ggggcatcag ggaagccatc gggcatctcg gcgccggtca
    12061 tcctctcgga ttctttccgt cgggagctgt ctcagacttc acccttagag acatgaaagt
    12121 aagggaccgc cggtggcagg aaagcatgct ccacctgatc cactcggtaa aggtaccggt
    12181 gctgccaatc aggttttttg acaagaactc ggctttcttt tacttcctcg gactgataaa
    12241 ctggagggtg cggctcctga gactgccctc agaggtgttt aacaaaaggg gcagtcatca
    12301 gagaatcggt atcggtgaac tgatctctgt tgaagaacaa tcccgctgcc ctgacccgtt
    12361 ttccctcggt gaatacctaa gaaaaaaagt atatgaaatg cctgtgccgg aacaattcgt
    12421 tcccggtaat ttaatttaac ttagaagtca aattactgtc atggcagcca ggagacccaa
    12481 caggaataac ctaaagtcac ttttcaacgg agaggttctc aggaatgtgt tcagtgacag
    12541 gaccaggaca aacctcctga agaaacagga agagatggca atctcatggc tggtacaacg
    12601 gatcccttcc ttcatcacct caaacatgct gacaggtatc gggttcttcg gaaacatgat
    12661 cgtatgtgca agcttcatcc tggcctcgtt ctacagcagg agcttcctcc tgctcggact
    12721 gatagggttt gcaataagct ggttcggtga ttccctcgac ggaaggcttg catactacag
    12781 gaacaaacca agaagaaaat acgggtttac ccttgacata acaattgact ggataagcat
    12841 cattctgatt ggcttaggct acatcatata tgccgaaggc gcctggaaaa tgctgggata
    12901 cggttttgta gtactctacg ggtgggagat gataattgca ctcatcaggt taaacatcac
    12961 aggcaagtat tcaatcgact ctgggaaaat gggccctacc gaggtcagga tagtcatcgg
    13021 actgataatg atcgctgaag ttctccttcc gggatcactt atctactccg catttgttgt
    13081 ggtaattgcg ctcttatttg tcaatgtcct tgataccaga tatttactca ggattgctga
    13141 tgacattgac aaaaaggaac acaaagagaa aacttagatt atctttatca ccacgcaacc
    13201 tgactgccat gggagaaatt accgtcaaag aggtaatcac gcgcaaggac ctccgtgatt
    13261 tcatctatct gcctgaaaaa gttcacagaa acgaacctga gtggcttccg ccaatctata
    13321 tggatgagtg ggagcttttt gacaaaaaga agaacaagtc tttcgggtat gctgacggac
    13381 tcctgcttac agcttacagg gacaataagc cggtgggccg gattatgggg atcatcaacc
    13441 gcaggtataa cgagatcaac catgagaacc acggacggtt ctgctttatg gaatgctaca
    13501 atgaccctga tgtctttcac gccctgatca gccacatcga acagtgggca aggcaaaacg
    13561 gcatgtcaaa acttgtcgga ccccttggtt tttccgacaa ggacccgcag ggattccaga
    13621 ttgagggatt ccagtacccg ttgttcatta cagctgccaa taactcccca ttcatggttg
    13681 agctgataga gaaggagggt tatgccaaaa aaatcgacct ggtgaactat cttggagata
    13741 tacccgggga gttcccggtt gtgtatgaac gggtccttga caggatcacc aaaagcaggg
    13801 agttcgaaat catcgaattc acaagcaaga agcaactgaa accctacatc cttcccatcc
    13861 ttgaggtgat gaatgacact ttcgcggaga tctacgggtt tgttccgctg aatgacaagg
    13921 agaaaaagga cttcgcggca cgctacctcc cgatacttga ccccaaattc atcaaagcgg
    13981 ttaagaagga tggcgaggtc ataggatttg ccatagggat gcctgacctc agcgccggca
    14041 ttaaagcctg caggggaaga cttatcccgt tcgggatctt cagagtcctc cgcgagtcaa
    14101 aacgttcaaa gaaactgatg atgatgctcg gcggagtcaa aaaggatttc aggggaaaag
    14161 gcattgacgt gctgatgggc gtgaaaatac tccgtgatgc catcaacagc cgcatggaga
    14221 ccattgacag ccacctcgtc ctcgaagaca acctgagaat gagaggtgaa tacgaacgga
    14281 tcggctgcaa ggtggtcaag aaatacagga tttaccagaa ggaactggtg tagaaacccg
    14341 ggtgatttct tttacttcag gcattcccgc cggatagtaa ctgtcctggc agaggtggca
    14401 gttgaaccta gtgcaggaag gtcacatccc ccaccatatc gaagtattct ccatcggcat
    14461 acctgcctga ggctttccag acataaacgc cctgcggggc caggttgccg tttccgaagt
    14521 aaccgtccca tcccttcttc aggttgtcgc tctcatagat cagcacaccc cacctgttga
    14581 agatctgaag cctgtattca attatattct caaaaaacgg cctgaaaacc tcatccattg
    14641 acggattcac gccctccgac cactcacctc cggtgggccc ggtgcggttc cacctgaaga
    14701 cattcggaaa cttcagggtt ccctccttcc actccaccac cacaggtgtg tccatctccg
    14761 cggaatcaac acacccctca tgagtggaaa caagcagtga gacagtataa gacccgggtg
    14821 actcatattt gtgccatgga ttttcctcgg cggaggtctc cccgtcaccg aatctccaga
    14881 gccatgattc cgcaaagtag gaatagctgt agaaaattac tatctgctca ttgttgacca
    14941 catcctttgg ataggcatcg aagatggcag acggattctg gtaaacctct ataactccgt
    15001 tacgaaccga ttcacccgca atgttgtgga cggtgagctt caccaggtat gtcccgggaa
    15061 caaaataagt gtgcacagga tccttttcag atgaatactg cctgtcaccg aaatcccacg
    15121 tgtagctctc gccgtgctgg ctcttgttta taaactgaac ggtgagcggg gcgcatcccg
    15181 atgtttctcc ttcaaagtcg gccacaggca tcggcggcat gatcacgctg acatatacat
    15241 cgcgctgtgc tgcacatccc cagctctcgg gaggaattga ataggtaagc atgtgattgc
    15301 caagcccggc ttcagacgga tcaaacagat tgcctgtcat gcccggccct gaccatgtgc
    15361 ctccggtggg gtaggccgtc agcgccaccg gcggaccatt gaggaagagg gtccccggat
    15421 catcaattat tattgaaggc agcgccatga ctgttaccac ggccgtgtca accgcagcgc
    15481 agtcagtgtt caggatttca tatttgataa tatggttccc tgtgccggcg agagcaggca
    15541 caaacctgtt gcccactacc cccggccctg accagacccc tcccaggtca tgtgccgtca
    15601 gcaggactga cggactgtct tcgcagaggg tatccagcgg ggtgatggta gcgtcaggcg
    15661 ttgcaacggt gaggatcgct gaatcagtat cagtacagcc gttggcatcg gtgatggagt
    15721 aagttatcag gtggtttccc gatccggcca ccgccggata gaaaagattt cccgagacac
    15781 cggtgccgga ccatacgccc cccgggtcat ttgcctgaag tacaataacc gggtcggttg
    15841 aacaggcgat cccggccgtg attatcgtgg catcgggaac ggggtaaacg gtaattgttg
    15901 tctgatcatc atcccggcat ccgtcggcat ttacaatctc atgccttatg gtgtggctgc
    15961 cgggcccggc aagtgaggga tcaaatatgt tgccgctcac cccgcttccg gtccatgtgc
    16021 ctcccggatc atgccccgtc agggctaccg gtgaggcatt tgcgcacatc gggtcaaccg
    16081 gcatgatggt cgcatcaggg tagggtacaa tcaatatcct tgcggtggtg actaccggcg
    16141 gatggtcacc gttgacacgg tcatgaggac cgccggggat agcggggtca tcatatggat
    16201 tacagtagtt ccagttgcga agggtaacct caaaatactg gcctataagc ttgtcatttg
    16261 ccacaaaaag gacatcggac cataccccgg aaccggtcac cgggccggta agtgtgataa
    16321 cattgtcaac gtagggaaat gtcctgctgt gaccgtcaac agtaaccgga ataccggtca
    16381 ttgtgatgtc tgtaccatag acccactgta tccacctggt gctgacgttg ggattatccc
    16441 tttcctgcgg aggcacacag ttaaactgcg taaggtcctg gaaacggacg ttggcgctgt
    16501 tgccgaaaca gatcgggtag accagcggat tgatatgcat atggcctcca ttgtgatcat
    16561 catcatccca tactgtcact atctgttcct gggatgatga ggtgcacagt accccgtcaa
    16621 ccaccagtgt ggcctgcggg tggtagttgc agatacttcc tgctgaggta taggtgttta
    16681 ttgaagttgc ttcaaacaca cccggggaag tttcagtggc agacagggtt tcaatattgc
    16741 cattgttcca gtcaaaccta attgtcaccg gtgcccctcc gtcatcaaca cccgtatagc
    16801 ttacggtcca ggtggcggtc accggtgaac aaagtttatc cggggttatg ttgttggcct
    16861 ttgatagaat tgagcagctc tgcccccagg cattcaacga caacaatacg gaaatactat
    16921 ataggatcca tttcttcatg aaccatctga ctacataccc tcgccggtgt gtaatgcaaa
    16981 atgttaaacg gccataaagg cttcagggtt acaattttcc gggccatttt cgcggcaaag
    17041 catgctgtat ctgttattaa atcactgatt tttagcgcct tcagcttatg tcggccgcct
    17101 gccggtctcc tttataaatt cttttcacta tcagcctgat tattgataaa gtctttatga
    17161 aacagtcgcc aaattagccg cacagtaaga aatcattata ccctttcaca acttaaattt
    17221 gcattgaaaa caagaaccgc atgaatggca ttctgacggg ccggctctca ggcacagtga
    17281 aacacatttt tcactatctg cttgtgatac tgataattgg tctcacaaca cttgtatgcc
    17341 tgccactgtc ctctccgcag agctatcaca tcgtatcatt tatactcctt tttgtggtag
    17401 ccattctggc tgttttcctc gggatagggc ccgtcctgct cgcttcaacc atgagcgccg
    17461 tttcatggaa ttacttcttc atccctccat ttcatacttt tcacattgcc aacactgaag
    17521 acaggttgat gtttgtctcg ttcttcatca tcgctctgct caacgggata ctcaccaaca
    17581 ggatcaggag acaggagagg gctgtccgcg acagggagaa tcagacaaat gccctgttcc
    17641 gcctgacggc cggactgtca ggagcgcgcg gtaccgatga agtcatcagg gtggctgagg
    17701 aacatttcag gacaaattca ggggccggag cccggttcat cattcaggac gggaacaata
    17761 acctggtctg ccagcctgcc gctgagtccg gcgaggatga tgaccagcct gatcatgacc
    17821 tggtcaaatg ggtgtttgcc aaccagagga aagcgggaag gtttacagga aacaggccgc
    17881 atgaaccgtt tacatacttt cccctgaccg gggcaatgat aaatcccggt gttgccgtat
    17941 gcaggatcgc tgaagaacgg tacaggaaca aggacgcctt ctgggatacc tgctgctcac
    18001 acattgcagg cgccatcgaa cgtgagttcc tgggtgagat ggctacaagg gcaagggttc
    18061 tggatgaatc agacaggttc tatggcactc tgtttaacct ggtttcacat gagttcagaa
    18121 taccaattgc ggctattatg ggggcctctg ataccctgat gtcctcagag gcttcggaag
    18181 aaaacaggaa ggaactgtat ggcgaaatcc tcaaggcatc agcccggctg aatcatatga
    18241 ccgaaaacct gcttaacatg tcacgtattg agagcggaag gatttccctc cggcttgact
    18301 ggcacgatgt gacagacctg tttcaaaggg tcaccctgag cctgaaacag gagcttgcgc
    18361 agtttcacgt gatcatgtca ataccggaag atatgccact ggtcaggatt gacttcagtc
    18421 tcgtggagct cattcttaat aacctattgc tcaacgcgac acagcactcc cccccgggct
    18481 cggccataaa aacggaggct tcatacgaga ataagggact agttatcagg gtatcggaca
    18541 gcggaccggg cttccctcct gcttcactcc cgcggctctt cgacaaattc ttccgcgttg
    18601 aaggatcgcc ggcaggcggt cttggcctgg ggctgtcaat tgtaagggga cttgtggatg
    18661 cacacagggg tactgtttcc gtttcgaaca tcagggaagg aggagccatc ttcactgtga
    18721 caattccgtc cgaaatcccg gacataaacc agcttaatct tgcagaatga atgataacag
    18781 gaccatactg atcattgatg atgaggtaca gatcagaaga ctgcttgaga tctcactctc
    18841 aacatacggg ttcaacaccg tctttgcaac taccggtaag gaaggcctgg tggcagcagc
    18901 aacccacagt cctgcgctgg ttatccttga cctgggattg cctgacatgg acgggatgaa
    18961 actcctggag acattaaggg aatggtatac aaagcccgtc atcatactgt cagtgagaaa
    19021 ctctgaggat gatatcatca aggcgcttga ccggggggct aatgattacc ttacgaagcc
    19081 tttccggggc ggggagcttg tagccaggat aagagctgca atcaggctga gcgaagaaaa
    19141 aaaggatacg ccgctgatgg aattcggatc gctgacaatt gacatgctca accacgtggt
    19201 tcgcaaaagc ggggaaatca taaagcttac ttccacggaa tattccctgc ttacactcct
    19261 ggccaaaaac accggaaggg tacttaccca ccggtttatc ctgaaggaga tctggggtta
    19321 cgggtacctg gaacagacac agtatctgag agtcttcatt gcccagctcc ggaagaaaat
    19381 agaagaaaac ccttcaaacc ccgtccttct gataaccgaa tcaggcattg gttacaggtt
    19441 tgggagctga atcgccggcc ggtagcctgt gatcataaaa cagccaggta tggcatcccg
    19501 gaaagtactt ctctttataa aatctttaca ggccctgact cattcttgat tttattctta
    19561 tgattatgga ctcaactttg ggccgcgttt gtaatgtaat ctatggatgc ttataaatcc
    19621 ggttcatatt gccagggtgc tgggcactat cctgcttatc gaagcagccg ccgtcatttt
    19681 ctgccttcct gtttctttga tttacagcga gcctgtatgg ccgtttgtta tttcagccgg
    19741 catatgcatt atcctgggag gagcgctttg gtttctgggc aggcgaccgg atcaaggcca
    19801 gatcaacaac agggaaacct acctgatagt cacatccgcc tggcttgtga tctctttcgc
    19861 aggcgcactc ccctacctca tcggaaaaac aattccctcc ttcgtcgatg cactttttga
    19921 gtctgcctcc gggctgacca caacaggtgc ttcgatactg acagatgtcg aggcacttcc
    19981 ccactcgata cttttctgga gaagcatgac ccactggatc ggcgggatcg ggattatagt
    20041 gctggtgatc ctcgttcttc catctctgaa aatgaccggc tatcagctct tcagcctcga
    20101 atcatcattg aaggaaaaga tacatccgaa ggccaagggg gtggtttaca ggatcctgta
    20161 catttatctc gggatcactg ctctggaaac gatcttcctg ctgctcgggg ggatggacct
    20221 gttcaacagc ctctgcaatt ctttcagcac cgttgcaacc ggaggttttt ctacccgtaa
    20281 taccagcgtg gcagagtatt caccatatat tcagtatgtt atcgcaatat tcatgttgct
    20341 ttccgcagta agttatgttg tcttctacca tctgctcaag ggcaacttaa agagggttag
    20401 gtcaaatgat gaactgtggg cttacctctt caccgtaacg gctggcgtaa ccgtattgac
    20461 aatcatactc tataccggca cagacagaaa ctttgccctt tcattcagac attcattttt
    20521 ccaggtgatc tcacagatct catgctcggg gtttgcaacc gctgactaca tgacattccc
    20581 tccgcttggc tggttcctcc tcttcctgct tatgttttca ggaggttcta ccggctcaac
    20641 aacaggcggg atcaagattg ccaggcacct tatattcttt aaaaacctgg gaaactcatt
    20701 catcaggctt catcacccca gcgctgtcgt ccctgtcaaa ctgaacggac agggcgtgcc
    20761 cgaaggggtg gtgaaccaaa caatggtatt catctcactt tactttgtaa tatatattgc
    20821 cggtgtattg cttttgcagt tatccggcgt atccatcctg gaatcagccg gggctgttgc
    20881 gacctcaatg gcgaatatcg gcccggggct gggccattcg ggaaacatgg gcaacttcgc
    20941 ccacttcaat ccttttgcca agacgattat gactctgctg atgattgccg gacgccttga
    21001 actcttcaca tttctgactg ttttcaccag gccattcaga aaaaattgac aaatcctaat
    21061 cagaatattt ttgtattttg atccgtcata tcgcacctaa acgcctgacg gatgaaaatt
    21121 acagtgaatg ataccctgct tgaccttcat aacggagcca gggtaagaga tgcaattgcg
    21181 ggtttttaca aacatgaagg acggaaggtg cctgaaaggc ttcctgttgc agaggaccgg
    21241 tacggcaata ttgtcgcgcc tgacggggca cttacagacg gtaatgccct gttcattctg
    21301 tcaggacgca aaaagagaac caggctgatc aggctgctcg ttgtcatcct ggccgccggg
    21361 ctcctcttcg cgtgcagcag cacaaggcac gccgccacct cccctgacat gaaaaaacag
    21421 ggcagcacca ccgttgcagc accggccggt actgttgctc cggatgaccg ccaggcggta
    21481 atcttctccg tcaatgacat gcatgctgca atcgacaatt tcccgaagct ggcgtggatc
    21541 gttgacagcc tcaggagtgt ttatccaaat ctgcttctca tcgccgcagg cgataaccag
    21601 accggcaacc ccatcaacga ccagttcccg gagaaaggcc ttccgatgat tgacctgatg
    21661 aatgccgtcg gtttcaacct cagtgcagtg ggaaaccacg agtttgatac cggccccgaa
    21721 ggattcagca acctgatcct taaggctaat ttcggtttta tctgcgccaa tgtatccggc
    21781 ggaggccggc attccctgaa aatcagccct tacaaaatct tcacccttcc caatgggctg
    21841 aaggtggcat tcctcgggct cctgcaactc gggcagaacg ggattccgga cagccatccc
    21901 gacaatgtca gggattttgt gttccgctca ccctgcgaga ctgccctcga atacctctgg
    21961 cttaaagaca gcagcgatgt tttcatcgcc cttacccata tcgggtttga ggaggatgtg
    22021 aaactggctg aaacaatgcc ggcagggatt gatcttatca ttggaggaca ctcacatacc
    22081 agggttgaca gggaacagat ccataacggt atcctgatta cacaggctga gaacaagctg
    22141 aaatacggca ccctgatcag actgaccgtt aacacagacg gccggatcac caggagcatg
    22201 caattgattg acatcaggaa ctcgaaaaag gaaaagcctg agatccgttc catggttgac
    22261 aggtacaatg ataacccggt tctcaaccag gtgatagcaa cagccactga cgacttctcg
    22321 tcatatgagg agctgggcta cctgatggct gatgcccagc gtgcagccgc cggggctgac
    22381 atcgcactga tgaacccggg gggcgtgcgt attgaccagc ttcccaaagg cccggttaca
    22441 atcaaaaatg tataccagct tgatcctttc ggcaatgaac tggtggttac aaagctcacc
    22501 ggcagtgaga tcttttccct tctgcgtgcc gcatggcctg tagacgaaaa atcacccgtc
    22561 tacacctcgg gtatcaccac cgggacaagg gtcaatgacc agggtgaccc ggaagagata
    22621 aaaatactca ccaatgacgg caaaccactg gatatgaacc aggtatacac cgtggccatg
    22681 aacagctaca tgacccaggt atacaggtac gagcacgctg atccgggcca gtcgctcttc
    22741 tttaccaccg ccgaggcgct gatcagatac ctgaaacagg aaggcaacct cagaagctac
    22801 cggggcgaga aaagggtcag ggttatcagg tgatcaactt tatgttctat ggcacatcgg
    22861 ccgcttcttc cttgatttta tcagtaataa ggcccacttt agccaattgt acataaagta
    22921 atttcaataa tggttaattc aaaaaatcaa aaacaacctg taaaacgaag tgcacatatg
    22981 ctcatctgca ttgtaatcat gacagttatg gttcagtgca gcaagagccc tgcagaagga
    23041 agatggacaa ccttctccgc agaagatggc ctggcaaata attggataac ggcaataacc
    23101 gttgattcca ggggcaacaa gtggtttggc tgcatgataa gaagcatatc atgctacaac
    23161 aacaggtcct ggaccaccta ttccgttcct gaggaatttg tctgcggata catcttcgat
    23221 attgcggttg acaaggacaa taacaagtgg atcggtaccc ttgggtcagg agcaatcaga
    23281 ttcgacgggg tcaactggac cagctataat gaatccaacg gcttcccggg taatgacgca
    23341 ctggctgtgg caactgactc tgacggcaat gtgtggtttg gtacccgcgg cggcgtggtt
    23401 agatatgacg gagaacaatg ggtcacattc actgatggtg acggcctcgt aagtgataca
    23461 attacggcca tggcagagga tcgcgacggg aatatgtggt ttggttcggt ctcgggagat
    23521 atatccagat ttgacggcac ttcctgggag aattttcaca ggcccgcaga ccccatgaaa
    23581 cacatcatca atgacatcgc tgcagatcag gatggcaata tatgggttgc caccgttttc
    23641 ggtgtgcttc gttacagtgg caccggctgg agagtcttca gcaaacatga cggactttgc
    23701 agctacggcg ccattgcagc agaagtggat aagaacggca ggaggtggtt cgcaactgat
    23761 gacggtgtct cttcatttga cggcattaac tggacctgct acaccgggga taacggcctg
    23821 gccagtaacg gagtcacctc aatagctgtg gaaccggacg gcacactgtg gttcggcaca
    23881 ggtgaggcag gtgtgtctag gctctccttc gaataatcag tatctttggc ctgaatgcag
    23941 gacaaggatt atattgtttt cgattttgac ggcaccatcg ctgataccct cgaattggga
    24001 ttgaatgtgt ataaccggat tgctccggag tataactgtc agcctgtcgg cgaaggggag
    24061 agggaactgt tccggacgaa aaagccccag gaactgcttg aagcctacgg gatctcgaag
    24121 ctgaagctgc tgacactgac cctgaggatc aggaaggagc tgaacaggca cgtgccggag
    24181 atgaagcttt tcgataatat ggaaaaggct cttcatgaga tccgtgacgc cgggtttggg
    24241 atgggtatac tcacctccaa ctctgtagag aatgtcagga gatttctgga aatcaacaat
    24301 ctgacggccc tgtttgattt catttacagc ggcagaagtt tcctcgggaa ggacagggtc
    24361 ataaggaaaa tgatcatcag ggagcaactc ccggcgggaa gaatagttta tgcaggcgat
    24421 gaaacccgtg acatcgaggc atgccgggca gccggcattc ctgtcattgc cgtttcgtgg
    24481 ggactgaacc ggagggaact ccttgcctcc cttttcccgg atcagattgc tgacaggccg
    24541 gaagaactgc ctgaccgtct gagacagatc ctcaggttta aaacagaaaa cagaagttga
    24601 ctgttatcct ttcagcctgg taagtgaaat tcagacgttt acctggtaac cggtactgca
    24661 gccatcacct gctgcaagca atgattcaat cgaattatga tactgcaact ctctggatgc
    24721 cacagcttta aaaaccttac ataaagcggt atattcccct gcattcatgc cgaaggtggt
    24781 ctgacagctc cggcggagag tggttaaacc caccggcgca tgcaccttag gtcaggtcac
    24841 ttcttttttg acacgagatc atatgagttg tcaacccatg aaaacacttc cctgtcagga
    24901 actgatccat caatgcgtac agtgttccag tgttttttgt tcatatgata gcccggcata
    24961 acggatgaat atctttccct tagctcaatc gccagtcccg ggtcacactt gagatttaca
    25021 ctgaggtctc cttcgagatt tgccagggca aaaatccttc cctgtgtttt gaaaaccagc
    25081 gtatcatcgc caaaagggaa gctttcggtg acatcctttt ttgaaataca gtattctctc
    25141 aggctttcaa tattcataat cagtaattta agaattctgc tatatgcctg aatgatggca
    25201 tagttaattg gcttcaccga ctttgcttaa cctgaacagt tttattgtaa acagtatcat
    25261 ccatgccata accataagtc cggcgggcat ggcaaatgca atagccatgg cttcaacctt
    25321 cgggataaat gtcaccagga caaggtagat gatcatcagt gagtttccaa ttattccgaa
    25381 ataagaggac agacggctga agatttttcc gctcagcata acgaatgaca taagcatgtt
    25441 cgcaaatgtc ggcaatgcaa atccgatgaa gacacccagg cttccatgtg aaccctttgt
    25501 cagcatcgct tctcctgccg ctgccagtaa cagtctctgt tcatctgaac ctgccgcaaa
    25561 gtatttccgg cttaaatcca gcattgtcaa cgccgtgttg ccggttacaa aaatcgttgt
    25621 ccctaccagg aacagcacca tggaaagcag ggcaaaggag ctgtttgtat tccggtgagc
    25681 tccgtacaga gcgattatga caggaattaa aataatctga tttatgatat tgagcaggtc
    25741 aaggttatat aatcccagga gccggttttc attgaactga ttgaaccgct caacggatgt
    25801 ctgaggcaat tcagccacat tgccgccagt tacggagccg actatcatgt cggtaacaat
    25861 cccgcataat acaataattg aggctattgc gcctgttttg aagattaatc tccactgcct
    25921 gtcagaggta tccatttgtc cgatctgcaa attatatggt tttggtgatc ctttcagggt
    25981 ttataccagc ttagcagctg tttccccaat aatcggattt ccttaaatca agacgctaag
    26041 gtaaagcagt aatttctttg ctgcaatatg ttctaatagt tcgagacttc cggattgctg
    26101 cagataatca ctgatacttc cggggaaatt aaagtattca ctgtctttga aaaacataaa
    26161 catcttttag ttgttatgtt taggacatgg gcaaaaacca aatgtatgtg aagatataac
    26221 cgggtcttcc ggcattgaag cccatcaatc ttttgagccg gtggacatga aacggcctgg
    26281 caattattaa agcacactca agaccgctgg tggcaagaat atcgtggttc tgaactaaat
    26341 acaaaataat aggaacatga aatcggcaat tctcttttca cttctcacag taacgtgctt
    26401 tttatactct aatgcacaga gtgtttcacc ggcaacggac ggactgttca ggctgtacag
    26461 cgattccgca tccctaaccc gggacgcagc ccccatggtg gctgatttta ataaccgggt
    26521 taaccggata cggcctgagc cggactttaa tgtcggattt attgtgtata ccactcccgc
    26581 tatggtttat tatgccccaa agagtaagaa tgtggttaca tcactttacc atgaactgac
    26641 ggatgatcac aaggcttttt ttaatacata ctcggacaat gaagaagatg ccagactgtt
    26701 ctttgccgtt ttttttaacg gattctatat cgctcatgag ttggggcacg ggctggtggc
    26761 tgcctattcg ttatcagacc ctgaagccat gtaccgcgaa gaatacgatg tcaacatgat
    26821 tgccatgaat tattggaaca gtgtggggaa agcggacgag cttgaaaagt gctacaaatt
    26881 tgccaaagca tttctggcca aagtgcccga ccctgtccct cagtgaactg aggactgtat
    26941 tgcatggttt aacgagcact actgggagtt aggacctcaa cccgaaaaat atggctactt
    27001 ccagttcagc cagttcgtcg acatttacga aaaccaaaaa agggttcata ttgacgaata
    27061 cctggaaact tacataagcc aattggaaga acgtgcgaaa caaaaaataa atacaaacga
    27121 ggaataatca actaacctga ccgatattaa actgactggt ttctttcaaa gacccggatg
    27181 aattattctt tctgacaacc tggtaaaatt ttctctgagt tagtttcctt ctgtgcgaca
    27241 atcatattgg tgctcaagga tgattttgat atatttgatt tcgacttttc gcttatgtaa
    27301 cttgaattaa gataattggc tttctgaatt gacaaaatga aaatttatcg tccttttctc
    27361 agcctcaccc tggttggcct tgcggtaatt ggttgcacga acaaagttcc tgattttgaa
    27421 agggctgcat tcaccataaa taaccaaagg atagaagtcc tgacaacatt tgatctgatt
    27481 gacaactact tgtcacactc cggtaagttt aacgggaata ttgtcaaatc ttcagataaa
    27541 tacatttata aaccaatccg ggatgaaatt cttgatgatg ccgaagcagc cttcctcttc
    27601 aataccatta aagtacctta tgaaacaaat gattatttaa gaaacgaaat acaattactt
    27661 aaatcgtctg atttattaac gattatacag gaatccttca ccaaaattac aaaagaactt
    27721 cccggaccag acactaaaat tattatcctg cctgcaagct cactgaaccg gccagcgctt
    27781 gaaaagatta agctgccaat aagcggaatc actcttggaa gtggcaaaat cattctttca
    27841 attgatccca cctttcaaaa ctggacagat ttcttaccct attgtatagc acatgagtat
    27901 catcatagca cctggtgttc aagaaattgg atatcagcag acttcagcct tatcgagtac
    27961 ctcgtatttg aaggtcgggc agatctgttt gcatcagaac aatataaaac tgtttcaaat
    28021 cccctcacaa attttttaac cagagagcag gaaaccgaag tctgggaact tattaaaaat
    28081 gaaatcttcg ggaagggaca tgacagaatt aacggagtaa tgtttggaac ggataaaata
    28141 ccatttggga gcggttataa tatcggattt aatattgtca ggctttttaa ggaaagaaat
    28201 cctgccacaa ataccctgga tctcatggat atgaatccaa atgagatttt tgaacgcagt
    28261 ggatatgaag actataaggc acaaaaataa ccggcagcca ttcagagcaa tgtcttaatc
    28321 caggctaata tccggacctg gcttcaaacc atcgccaacc tccccctctc aggtcagggg
    28381 aaagtataaa aactgatccc ggagaccaga tcttcaggtt ggatggagtc tggtgagggt
    28441 cgtacgaaga tcgaaggtct gagctaacca gaataggaaa acttacattt cggtttaaaa
    28501 aatggatact gtctgcagcg aattggcgtc cattctcgta ttaatgatat atctcatcaa
    28561 ggacaattgg gataaaattg acacttgatc aagagaccga ccaacaatga aacccataaa
    28621 gtatctactc gctaccataa ttctttttct aattgcatgt gaaaaaattg actatgaaca
    28681 aattagccct gatagcttca atgacagcat ttctgctgtg tggctgcaaa gaagtgccag
    28741 ataattctga agatgatctg acaattgaac tggagggatg ggagatttta aaatcaactt
    28801 atgattttga catcaatccc agggatatgt atttcgttga cgagagcatt ggcttcgttg
    28861 tcggatacaa cggcgatatt tataaaacaa ccgattcggg cactacatgg cgaaaactga
    28921 attcgggcac tacattacat ctgcattcgg tctacttta
//
LOCUS       DAOPXU010000029        28129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_1676_length_28129_cov_13.250944, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000029 DAOPXU010000000
VERSION     DAOPXU010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 28129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28129
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1676_length_28129_cov_13.250944"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..273)
                     /locus_tag="PKK03_04545"
     tRNA            complement(200..273)
                     /locus_tag="PKK03_04545"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(237..239),aa:Ile,seq:gat)
     gene            473..1207
                     /locus_tag="PKK03_04550"
     CDS             473..1207
                     /locus_tag="PKK03_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005780203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNX83697.1"
                     /translation="MPNLVIVPTYNERENIPGLVRAITGLSVPFDILVIDDNSPDGTA
                     SIVKEMMVSFPNLHLLERPGKMGLGRAYIAGFEWALQRDYSYIFEMDADFSHNPDDLV
                     RLWDACENGGADLAIGSRYISGVNVVNWPMGRVLMSYIASMYVRLVTGMKIMDTTAGF
                     KCYRREVLLNINPGHIRLVGYGFQIEMKFTAWQLGYKIVEVPIIFTDRKAGTSKMSGG
                     IFGEAMWGVLRMKIVSIFRKPARTVA"
     gene            1218..2576
                     /locus_tag="PKK03_04555"
     CDS             1218..2576
                     /locus_tag="PKK03_04555"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF006688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNX83698.1"
                     /translation="MQEKAENKTVIRNALLINEESMRKADVMIAGDRIAGIFPPGKAP
                     SGAGTKMIESSGLWLMPGIIDDHVHFREPGLTHKADIASESAAAAAGGVTSFMEMPNT
                     LPQTISISEWEGKNRLAAEKSVINYSFYIGATDSNIDELRMADPSLVPAFKLFMGAST
                     GNMLVSDSSTLEAIFRLRKIPVACHCEDEKIIKANIAAYREEYGVTIDPAFHPLIRSR
                     EAAIASSETAVGLARHGGARLHLLHLSTAEETQLLTPGADPAAKQITGEACVHHLWFD
                     DSSYASKGNLVKWNPPVRSAADREALLSAVRKGLIDVIATDHAPHTLEEKSAPYFKAP
                     SGGPTVQHSLVMMLELCHRGELNPELVVRKMCHNPALLFGITDRGFVREGYKADLVLV
                     DPAAPWTVNKGNILYKCGWSPLEGTTFRSSVITVFANGVPVFEEGGLTGRRAPEMLRF
                     KR"
     gene            2589..3200
                     /locus_tag="PKK03_04560"
     CDS             2589..3200
                     /locus_tag="PKK03_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83699.1"
                     /translation="MNRIALFIILIALFTSCGEGGREKGGASTAGAGGTEAGDSANTK
                     SQGGTSAMNRAGADSSTALNRSAGTELHAGAVLIASDIVTEVIIKPDPEGDPWEVEKV
                     AGYNGQVLVDAVFSRIYDGSMTVYDYHSGAPLSPADVKKIESEFKNDRTKIGKISFTE
                     DWYYFPEDNRVEKRAKSVTFGYELYNNTGKVYAYRAAFRADLN"
     gene            3228..4232
                     /gene="fbp"
                     /locus_tag="PKK03_04565"
     CDS             3228..4232
                     /gene="fbp"
                     /locus_tag="PKK03_04565"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNX83700.1"
                     /translation="MPSYDITSLNDFIISKQKDFPFAQGELSSLLHHIGTAAKMVNAK
                     IRKAGLVEILGRAGAENPQGEEQQKLDRYADEVFIDALKSSGQCAGIASEENQNEVVF
                     TGEFASRGKYVVCIDPLDGSSNIDVNVSVGSIFSIYRRVTERDEEVSIADFLQEGSRQ
                     VAAGYVIYGSSTMLVYTTGNGVNGFTLDPTIGEFCLSHPNMKTPETGRIYSINEGNYI
                     KFPEGVKKYIKYCQENDPATKRPYISRYIGSLVSDVHRNMLTGGIFLYPPSISYPSGK
                     LRLVYECNPISFIIEQAGGVATDGTRRILSLKPESLHQRTPFYCGSPDMIRKLVGMCC
                     "
     gene            4299..7676
                     /gene="mfd"
                     /locus_tag="PKK03_04570"
     CDS             4299..7676
                     /gene="mfd"
                     /locus_tag="PKK03_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNX83701.1"
                     /translation="MASTVFRDIYRQHPSFRRLEELLAGDSAEPVTIHGLTGSSRSIV
                     MAGALSQQPTTHVVLLPEKEDAAYFYNDLTSLMGDDNIFFFPGTYKRSVMYDQTEPAN
                     IILRAEVLNFLSSGRRKCIIVTYPESVMEKVISKSSLKKNTFFIKKGAKLSIEFLEEV
                     LREYNFRRTDFVYEPGQYSVRGSITDLFSYSADKPYRIDFFGEEVDSIRSFSPDDQLS
                     LQLHDEIQVIPDIQLLAFQESADSFIDFIPPSSIIWSDNFDYIVEKTDNIWAQARSRS
                     SKGEISDRRDIIITGRHLEELCSRHRRIFFGRGRQDPDTVNIGFRTEPQPAFNKNFGL
                     LTSKLASNVQEGYSNFIISESEVQEQRLKEIFEETDPAVQFTPLLLNLHQGFTDHDLK
                     IAVYTDHQIFDRYHKFRIRGFFTRRDSMTVRELTDLNPGDYVVHTDHGIGRFGGLEKI
                     EVNGRMQEAIKLVFKDNDILYVGIHALHRISKYKGKDDTPPRINKLGTGAWQKVKQAT
                     KKKVKDIARDLILLYAKRKASGGFAYSPDSYLQRELEASFIWEDTPDQLASTVAVKED
                     MEAPVPMDRLICGDVGFGKTEIAIRAAFKAATDGKQTAVLVPTTILALQHFRTFSSRL
                     ENFPCKVDYISRHRKPAEQKKILKQLSDGEIDIIVGTHKLTGKEVMFKDLGLLVIDEE
                     QRFGVAAKERLKTLRAGVDTLTLTATPIPRTLQFSLMGARDLSVISTPPPNRMPIITE
                     LHGFNEDIIREGIVYEVSRNGQVFFINNRIENIMEVKASLLHFCPEIKIAVVHGRMEG
                     DEIENVMSDFIRGDYDVLLATTIIESGLDIPNANTIFINDAHNFGLSELHQLRGRVGR
                     SNRKAFCYLLTPPLSVLTGDARRRLKAIEENYELGSGFNIALQDLDIRGAGNLLGGEQ
                     SGFIADVGYETYQKILDEAVRELKDEEFGGLFPGPAEKKEHAAEEKPKQFVEDVTVET
                     DLEIMFPDDYVSNIPERIRLYKELNDIRDEAGLKAYESHLTDRFGPPPPPALALLQLV
                     RLKWAAARTGIEKVILKNNTLIANFVTDQSSQFYRSPLFVSVMNYVNRRQGNMSVKQN
                     NNKLSLTVRSVTTVVQAIDLFTAILAWHESAQASKAN"
     gene            7680..8399
                     /locus_tag="PKK03_04575"
     CDS             7680..8399
                     /locus_tag="PKK03_04575"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNX83702.1"
                     /translation="MNLIIDIGNTSVKAALVSKGVIISQERFGSTDPEMIGMFTGRRR
                     LSGAIVSSVGGDPLGLLDWLRTRAGRVHHLTSQSQYPFAIDYATPETLGTDRLAAAAG
                     AVDHHPGADLLVIDAGSALTVDQVTGGVYRGGSISPGLAMRFRALHEFTGRLPLVTKR
                     TGFTFPGKTTEDAIAGGVITGLVYEIIEYIRTFEEKHHNSVAVITGGDSGLVTPLAGG
                     RLLHYPDLVTEGLNFLLETNV"
     gene            8416..9663
                     /locus_tag="PKK03_04580"
     CDS             8416..9663
                     /locus_tag="PKK03_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83703.1"
                     /translation="MISLLALLATGAAGQKTVNTPFARYGIGTLELQGAFRTRAMGGI
                     SSGIRDNLTLNYLTPASYSSTDTTSFLFDFGIDYAVAKLKGDDLRYNSQDMTFSHLMM
                     GFPIMKGWGVAAAVLPYANGSYDIGYKSEGTGVTGSIYELHNGSGGFQKALLGTGVRV
                     LPFVSVGANVFYMFGEETRINDFVFTGDNNYYNTRKQGTSGMRGFGYDASVQFMLNLS
                     GGRFINAGLTYTPAYDLKTNNEDIIMRYSTVSNSIYSFDTLTYGKLSTASRMPQSLRA
                     GFSLGKVDKLTAGADIVYTRWTKASLPGTYGTYTDALALHAGAEYIPERYSNYSFFDR
                     VEYRIGCRYGESYTLFGGDELKEYGITFGTGIPMKRSRSRISLTVDLSTRGGNGSIPQ
                     ETRVSVGASLNLYDYWFVKRQYD"
     gene            9682..11010
                     /locus_tag="PKK03_04585"
     CDS             9682..11010
                     /locus_tag="PKK03_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83704.1"
                     /translation="MKRTTSTIFTGLLLSVFSLALQAQKGVETGTPFGRGEDSTRCLR
                     NTSLYSTYYENKDYNMAVQFWRPVFYECPGSSKNTYIKGETMFKEFFRKTGNKAYIDT
                     VMMILDQRTKYFNEEPANNLRKAFALYEFGGNDADYARRCYDIIKGVMETSPQSFDHT
                     YSSLYMAITAKCYALKLIEAPEVITAYSQSMKVVDNQLSRKPGDARFTEARKNIDAVF
                     RSSGAASCENLEHLFTAGVDNNPGDTAMLRKVLTLLGETGCKDSELYYKAATNLYRSD
                     PSASSAAQLAEMNLARKKYNEAEKYYTEAIDLETDSKVKSGLLTKLATLELTSDSKQA
                     ARDFARAAFSLDPTNGNALFIIAEAYAGSRIGETFENQTVYWVVVDYLIRARNTDPTL
                     KEQVDERIGIYSRLFPTKEEAFFRSLVEEGASYQVGGWINESTTIRFRKE"
     gene            11043..11594
                     /gene="lptC"
                     /locus_tag="PKK03_04590"
     CDS             11043..11594
                     /gene="lptC"
                     /locus_tag="PKK03_04590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNX83705.1"
                     /translation="MIAVLVAGTALLSSCDKKETTVKDLMIKGLPTLSSRNVETMYSD
                     SGKVTMVVRTPLIQQFTSEENPYTLFPEGLTVLFYDRTDVPQASITSHYARYTEKDDV
                     WELRDSVVAVSNKGEVLETELLFWSQPRDRIWSDRFVRFTHNDQIVMGTGFDSDSRFS
                     NWTIKNVTGTIYIRDEQEVTPAD"
     gene            11566..11748
                     /locus_tag="PKK03_04595"
     CDS             11566..11748
                     /locus_tag="PKK03_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83706.1"
                     /translation="MNKKSLLLIEIIWIILGLMCLGIAIRETVNAGIGRAWLFYVMAI
                     AAFVLAWIRDSQRKKL"
     gene            11758..13017
                     /locus_tag="PKK03_04600"
     CDS             11758..13017
                     /locus_tag="PKK03_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNX83707.1"
                     /translation="MNPWVIIPFAILLAAFFSAMELAFIASNKLRIELDRKQGNFGSG
                     IIALFSRNPGLYISTMLIGVNIATVIFSMLAADLMESWFKNLIPSEVLIFIIQTVITT
                     MVILVFADFLPKSVVRISPNFFLRIFAVPTAIFYYLFYPVSKFTLWLANMIMRIFFGI
                     KTERKEQENRIFTRIDLDHFVNDMVETDEEKEEENSSLRIFQNALDFSSVKARECMVP
                     RTEIVALPVHSSPDELRHQFIESGHSKILLYRDNIDNMVGYYELKDILHGPADIATGM
                     RKLPVVPETMTANRVLRLLSDAKKSIALVVDEFGGTAGVITIEDVLEEIVGDIEDEHD
                     ISDLVEKKINEKEYVLSGRLDIDYLNEEYDLGLPESDEYTTLAGLILFAHGNIPRQQE
                     KIRVGRNLFTVLRSSSTKVELLKLQID"
     gene            complement(13163..14539)
                     /locus_tag="PKK03_04605"
     CDS             complement(13163..14539)
                     /locus_tag="PKK03_04605"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNX83708.1"
                     /translation="MESIKNYIETNRDRFIEELFGLIRIPSVSASESSKADMVRAAEY
                     IRDLMAANGADQATVYATPGNPIVYGEKMVDPSLPTILVYGHYDVMPAEPLEKWTSKP
                     FEPEIRDGKIWARGADDDKGQLFMHLKAFEMMNATGTLACNVKFMIEGEEEAGSASIA
                     GFCAEYSETLRSDVILVSDTTMISPEIPSITSGLRGLSYIQVEVEGPDHDLHSGIYGG
                     AVVNPCNALCDIIARLKDSDGRVTIPGFYDNVHVPSDAERAKMAERPFDPEKYRKSVG
                     VDALAVEKGFSTLESTGIRPTLDVNGIWGGYTGEGAKTILPAKASAKISMRLVPYQKA
                     GDIAELAINHIKSLAPAGVKVSAEYLHGGEAYVSPLDTPEYLAAEKAYTEIFGRKPIP
                     IRSGGSIPIISTFEKILGVKSILMGFGLESDAIHSPNENYPLENLFAGMRAIPLYFKY
                     YAELKKKG"
     gene            complement(14526..15125)
                     /locus_tag="PKK03_04610"
     CDS             complement(14526..15125)
                     /locus_tag="PKK03_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HNX83709.1"
                     /translation="MRETMLQIDDTIVSLALIEKKFVCDLAACKGSCCRYGDTGAPLT
                     AEEASALERIWPDLWPWLRPEGIRAIEKQGTSITDIEGELVTPLITNEECAYTIMDDD
                     VYKCGIETAFNAGAVDFRKPLSCHLFPVRVKQYSTFRAVNYEEWFICKSGVACGAKQN
                     TELYLFLREPLIRAFGEEWYNRLHWAAGEYHKYNDNGKH"
     gene            complement(15122..16642)
                     /gene="gpmI"
                     /locus_tag="PKK03_04615"
     CDS             complement(15122..16642)
                     /gene="gpmI"
                     /locus_tag="PKK03_04615"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNX83710.1"
                     /translation="MKENKILLMILDGWGIGDGSRSDVISTTGAPHIEALKKAFPSSQ
                     LLTSGEDVGLPEGQMGNSEVGHLNIGAGRIVYQDLVKINKACRTGEIRTNGTLREAFT
                     YAREKGVKVHFMGLLSDGGVHSLDSHLYALCDMTMDYGLKEVYIHGFGDGRDTDPKSG
                     KGYMANLLKHLEKSNGRVASFVGRYYAMDRDKRWERIREAYEMLVHGRGTQTRDILQA
                     MQESYDAGVTDEFMKPVVVTGADGHPLATIAEGDVVVFINFRNDRARELTIVLTQQDM
                     PEAGMKTIPLHYLTMTPYDDKFRGLHVIFPKENVDRTLGEIIADAGKKQLRIAETEKY
                     AHVTFFFSGGREKEFQGEERILIPSPKVATYDLQPEMSAYGVRDAIIAELRKKKFDFI
                     CLNFANGDMVGHTGIYEAIQKAVRTVDECAGDVAEAATANGYEVIIIADHGNADYAVN
                     PDGSPNTAHSLNPVPLYLISKRYKSVRAGILADVAPTILTMMGIPVPPEMTGKSLV"
     gene            complement(16742..19750)
                     /locus_tag="PKK03_04620"
     CDS             complement(16742..19750)
                     /locus_tag="PKK03_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83711.1"
                     /translation="MPERNIIIRPNPFPGLRAFRPDEGHLFFGRMESTLKVVSKLKEN
                     RFVAVLGASGSGKSSLVLSGVIPALLKENTEEKKSWSYLVFRPALNPVDHLATELSVL
                     SAGAGFNQLSDVSVAASLHNRTEGLNDVINRIRKNLRQQVVIVIDQFEEIFRFSPATS
                     RGLPGDDATDFIDLIVNAMQKPDHGLYIILTLRSEYVSDCGRFHSLTDLMNSSSYLLP
                     QIPVDQMVTVIEEPVKLSGGSIDRSLVKLILSDLEERPGQLPVLQHLMMRTWNQWSRL
                     GDLSRPIGIADYEAVGMLKGAISQHAGQAFDSLDERHRYVCSRLFRTITTRSDDGREL
                     RKPERISTIAVLTGCAEHEIIGVAEVFRSPEYSFLTPSKEVPLNGESILDLTHESIIR
                     LWGNLRKWMDEEEASVKLYRQLASAAAQYQEGTGRLWTAPDLLMALRWRDENNPTLAW
                     AEKIDPAFERALIFLKNSEEEYLIREEYGRKSSNETVRRSRLIAGLLGLLAILTLVAL
                     GAVYSLRNRAERQKSVAIQMKDEAVTLNDRLSDSLEVLSSALGLAPAATEGNLIMPEE
                     RVKQAEARARSAEEAVREMASGKAEDMERFSKANSNRMLSLARSLAVRSLNHAGNKDL
                     QILLAWQAYLFNRKYDGIAEDADVFAGLYDVSKRYGNRFCARYTPDGAEYTAMAEGFD
                     GIFFTADSKGRVLSWKAGQPSAGYDLVWSGNKTISAMTLSPDRSWLACGTDDSEILMI
                     PFDDDSIGYQLHDSTGTITALSYTDTDHLYSSTSGGSVNEWDLRNRSVRKVASDGSGI
                     VALEISDDNNMLAALTGNGRLICWQTGSPAKHITINTGDKIITAHKFIPGGNRLATGD
                     DTGTIDIWSTSTGLADGNASGHMSAVRGIAFDVADGQMLTADEAGEVRLWTMANLEQP
                     PVVFTDGGQEVLRLAFSDGGESFLAATQTDVIRRPAHIRLMTDGLCDKVTRNLTMQEW
                     SAYVGRDIEYETTCPDKEYRIRVREIRGAR"
     gene            complement(19762..22089)
                     /locus_tag="PKK03_04625"
     CDS             complement(19762..22089)
                     /locus_tag="PKK03_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83712.1"
                     /translation="MKTGFNWVFLTALVLMALTAAAMVAVLTDLSLFRATPLLYTLTL
                     SVIVISTGLVIRNRYYLPILRNLVLRGKLLLAPMVLLALTIPAALIYIYYDLTGRESL
                     LLLSALVTSVAAAFFLPSMAMITLPALASVSHLAEKAGLPSGRIKAEDIARQTVIAAA
                     FIAVILVVVTITADLRISGIIFLFPVAAIALMLLDIVIVAHFRNRLAMMLDAPVPAET
                     SGAGQNQATEKSEGFMSVMLVADHYPDLIGGRLEYLLNNAGDFYAMEVISLASKTYDP
                     ALLPAMKAIASASRFSDKLRHEAANGVSVIEKYYSDPVRNVDFLRLPGIPEKAAAARA
                     IFLSKSKPSEQEIIKLLRDASAEVRRTGLMAAGRYGMTSLRDEVMKGLDNPDTAREAY
                     YVLRQFGPEVYGDLIGTVIRPGNSERENYIILRLLDAMPASEAFPWLSDFVVAGHMGV
                     RLKAASSLCNRGWSPQGRQRLKIGETLSETIHVMARLIAMQTEVSRSRHFLLSAALEQ
                     ERENNYELIRCLVHLLAGGVAAELILPRKRDDRPCQAGVASEAIESVISEPMRRPLKA
                     LLGNSTDNRRLAELSLYFPVRSVKGQSISSFLLASEQNITGTWSKACALHLAAAEGRG
                     LDREHAISYLFSNSQILQEESARVIRSINPDWYRDAELRLPDPARARISAVTGGTLPQ
                     AAMSFEKTRFLSLCFSKIPEETMIMLASHLRYSGSYDAGSLPGVISWVVPSRDGKTGL
                     YSIPVNDIAAFVFYYPEFTDIFVNYTDSQEGVPAI"
     gene            complement(22164..22691)
                     /locus_tag="PKK03_04630"
     CDS             complement(22164..22691)
                     /locus_tag="PKK03_04630"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNX83713.1"
                     /translation="MNIYLIGFMGSGKSSAGKGIAALLRWRFTDLDKLVEEKEGMTVA
                     GIFATKGEEYFRKAEAEALQDVSGRTRTVVACGGGTPCSDKNISLMKATGVTIYLRLT
                     VDALVSRLSRSRTDRPLLKDAGPDELHSIVQKLMGKRSSWYEQADLILDSETMGEEEM
                     TAMIAGTVRSKGAYL"
     gene            complement(22698..25346)
                     /locus_tag="PKK03_04635"
     CDS             complement(22698..25346)
                     /locus_tag="PKK03_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HNX83714.1"
                     /translation="MMKESDPFELIPEEEGGEKEVPKTGYIPEALHTVTALSGMYQDW
                     FLDYASYVILERAVPHMSDGLKPVQRRILYSMKRMDDGRFNKVANIIGHTMQFHPHGD
                     ASIGDALVQLGQKELLVESQGNWGNILTGDGAAAPRYIEARLSKFALEVTFNSKTTEW
                     MSSYDGRNREPVTLPVKFPLLLAMGVEGIAVGLSSKILPHNFNELIDASINYLQGKPF
                     ELYPDFPTAGMIDVSKYNDGARGGVVKVRARIEKVDKKGLVISEIPFGRTTTSLIETI
                     VKANDRGKIKIKKIDDNTAGGVEIVIQLQSGISPDKTIDALYAFTDCELSISPNACVI
                     TDENKPAFVGVSDILRHNTDQTVKLLKLELEIRKAELEEEWHFSSLEKIFIEKRIYRD
                     IEECETWEAVITAIEKGLKPYKKLFRREITRDDIIKLTEIKIKRISKYDSSRADEHIR
                     GLETDLEEVANHLANIIPYAINYYRQIRKKYGKGRERRTEIRSFETIEATKVVAANAK
                     LYVNRQEGFIGTGLKKDEYVCDCSDIDDIIVIRKDGTYLITKVAEKQFVGKDVIHAQV
                     FLRNDSRTIYNVAYQDGREGPYYVKRCALTGLTRDKEYNLGRGTAGTRILYLSANPNG
                     EAEVIRVYHKPKARLKKLTFDFDFGELAIKGRGSMGNILTKNAVHKITLKEKGLSTLG
                     GRKLWFDDAVMRLNADGRGKFLGEFASEEMLLVVTRGGWYRTSGTDLSSHFEDDLLLL
                     EKFRESKIFSSVYWDEEQQFYYVKRFAFEEADRLQCFISDSEGSRLVSLTEVEYPRFE
                     IKFGGKNEGRKQEIIEVAEFIGVKSFRAKGRRLSNYSVADIREIDPVVRSEAMPVPPE
                     PENGTADESPLQDHTPDDPAQMKLDL"
     gene            complement(25363..27210)
                     /locus_tag="PKK03_04640"
     CDS             complement(25363..27210)
                     /locus_tag="PKK03_04640"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNX83715.1"
                     /translation="MDGNYSEENIKTLEWKEHIRLRPGMYIGKLGDGSSLDDGIYVLL
                     KEVMDNAVDEYMMGFGRRIDVSIVGSEVRVRDYGRGIPLGKVLDVSSRMNTGAKYDSK
                     AFKKSVGLNGVGIKAVNALSSSFVIRSFRDSQLKEIEFRCGELVRDEPLRKTEEENGT
                     EVIFVPDEAMFGNFHYIAEYVESMMKNYSYLNAGMIINLNGNKIVSRNGLVDLLNDNM
                     NCEGLYPIIHLKGDDIEVAMTHGTQYGEDYYSFVNGQHTSQGGTHLIAFREAITRTVK
                     EFFKKDFDVSDIRSSIIAAVSVKIEEPIFESQTKTKLGSKDMGPNGPSVRNYVVDFIR
                     KHLDDYLHKNGETARIMLKKIQESEKERKAISSIQKLARERAKKVSLHNRKLRDCRIH
                     YNSNDERRLESTLFITEGDSASGSITKSRNVETQAVFSLRGKPLNSYGLTKKIVYENE
                     EFNLLQAALNIEESLDDLRYNYVVIATDADVDGMHIRLLLLTFFLQFFPDLVRKGHVY
                     ILQTPLFRVRNKKLTRYCYSDEERQKAIKDLGPDPEITRFKGLGEISPDEFIHFIGKD
                     MRLEPVIMKKADPYTQFLEFYMGKNTPERQDFIIDNLRVEEEIPEAAQA"
     gene            complement(27369..28106)
                     /locus_tag="PKK03_04645"
     CDS             complement(27369..28106)
                     /locus_tag="PKK03_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNX83716.1"
                     /translation="MNLIKSLWVWFSSITFILLALPVAVILWLLALAFDRRRLMNNRW
                     MVIQGIVLTKMSPFWKVIVDGREKLDQKQAYIIIPNHQSLLDIVFFNMLRHRLRWVSK
                     DEIFRVPVVGWEMKMVKYIELIRGNKSSVVKMMEKCVESLHDGISVVIFPEGTRSLTG
                     AIGKFKTGAFQLAVKTDKPLLPVLIDGTGEIMPKKGGIIFRNRRIVRIRVLDPIFPGQ
                     FGTGDPEELATRVQSIMVTAMDQLRNE"
BASE COUNT         6723 a         7447 c         7165 g         6794 t
ORIGIN      
        1 tgctctggcc aactgagcta aatccccaat tttgtaaaga accggattca gaacccttga
       61 ccctcccgat ccgccaaccg gcggatacgg gatgctctgg ccaactgagc taaatcccca
      121 attttatttt cgactttcga ttcccgattt tcgagttaag caacaatctc aaatcgcaat
      181 tcgcaaatct caaatcctgt agtcccaccc agagttgaac tggggacctc tacattatca
      241 gtgtagcgct ctaaccaact gagctatggg actgataagc gcacactata aagaacaccc
      301 cgtttttgaa tcatttgggg tgacaaaagt aacgcttcct tgtaaacatc caaaattttt
      361 tcaaaaatat tttttcagcc ctgccaaaac caaagccctg gacccaggtt cagcatggta
      421 ttgcgggaca gagtaataaa aataattaac ttcgcattca tagcggatca atatgccaaa
      481 tcttgtcata gtacccacct acaacgagcg ggagaacatc cccggcctcg tcagagcaat
      541 aaccggtcta tccgtaccat ttgacatact ggtcattgat gacaactctc ctgacggtac
      601 agcctcaata gtcaaggaaa tgatggtctc atttcccaac ctccaccttc tcgaaagacc
      661 cggcaagatg gggctcggca gggcatatat cgcaggtttc gaatgggcac tgcagagaga
      721 ctacagttac atattcgaga tggacgcaga cttctcccat aatcctgatg accttgtcag
      781 gctctgggat gcttgtgaga acggcggtgc tgacctggca atcggctcaa ggtacatctc
      841 cggcgttaac gtcgtgaact ggcctatggg aagggtgctg atgtcataca tcgcctcgat
      901 gtatgtgaga ctggtgaccg gcatgaagat catggacaca acggcaggct tcaaatgcta
      961 ccgccgcgag gtattgctga atatcaatcc gggacatata aggcttgtcg ggtacggttt
     1021 ccagattgag atgaagttca cagcatggca actgggatat aaaatagttg aagtgccgat
     1081 aatcttcact gaccggaaag cgggaacctc aaaaatgtcg ggaggcatct tcggcgaagc
     1141 catgtggggt gttctccgta tgaaaattgt cagtatcttc cggaagccgg ccaggacagt
     1201 cgcctgaccg tcttatcatg caggaaaaag cagagaacaa aacagttatc cgcaacgcac
     1261 tgctcatcaa tgaagagagc atgcggaagg cggatgtcat gattgcgggt gaccgcatag
     1321 ccggaatttt tccacccggg aaggctcctt caggcgccgg gacaaagatg attgaatcgt
     1381 cagggttatg gctcatgccc ggcatcatcg acgatcatgt ccatttccgg gagccggggc
     1441 tgacgcataa ggcggacatt gccagcgaga gcgctgccgc tgcagccgga ggggtgacat
     1501 cgttcatgga aatgccaaac accctgccgc agacaatttc catcagtgag tgggaaggga
     1561 aaaacaggct ggcagcagaa aaatctgtaa tcaactactc attttacatc ggggccactg
     1621 acagcaacat agatgagctc cgcatggcag atccatcact ggtgcctgcc ttcaagctct
     1681 tcatgggtgc ttccaccggc aacatgctgg taagtgacag ctcaacgctg gaagccatat
     1741 tcagactcag gaagatcccg gtggcctgcc attgtgagga tgagaagatc atcaaggcca
     1801 atatcgccgc ataccgcgaa gagtacggtg taactataga tccggcattt catccgctga
     1861 tcagaagccg tgaggccgcc atagcctcat cggaaactgc agttggtctt gcccgccatg
     1921 gcggtgcgag gctgcacctg ctccacttgt cgactgcgga ggagacacaa ctgctgacac
     1981 ccggagccga tcctgccgcg aagcagatca caggcgaagc atgcgtccat cacctctggt
     2041 ttgatgacag ctcatatgct tccaaaggaa acctggtcaa atggaatccc ccggtgcgca
     2101 gcgcagccga cagggaagcg ctgttaagtg ccgtaaggaa ggggctcatt gacgttatag
     2161 ccacagatca tgctccccac actcttgagg agaagagtgc cccttatttc aaggcacctt
     2221 cgggcggtcc cactgtacag cactcactgg tgatgatgct ggagctgtgc caccggggcg
     2281 agctcaatcc ggagctggtg gtgaggaaga tgtgccataa cccggcattg ctcttcggca
     2341 tcacggaccg gggctttgta agggaaggat acaaggctga tctggtcctt gtcgatcctg
     2401 cggcaccatg gacggtcaat aaaggtaaca tcctttacaa gtgcggatgg tcgccactgg
     2461 aaggaaccac tttccgaagc agcgtcataa cagtatttgc taacggggtg ccggtatttg
     2521 aggaaggcgg gctgacaggc cgcagggcac cggagatgct ccgtttcaaa agataaaaga
     2581 gaagcattat gaacagaatt gctttgttta tcatactcat tgcacttttc acctcgtgcg
     2641 gggagggagg cagggaaaaa ggaggggcct ccactgccgg tgctggcgga actgaggccg
     2701 gagatagcgc caacacgaaa agtcagggag gaacctcagc gatgaaccgg gccggggcgg
     2761 acagcagcac cgcactgaac cgcagtgccg gcactgagct ccatgccggc gcagtgctca
     2821 ttgccagcga catagtcacc gaggtaatca taaagccgga tccagaaggt gatccatggg
     2881 aggtggaaaa ggtggccgga tataacggcc aggtcctcgt tgacgctgta ttcagccgta
     2941 tctatgacgg aagcatgact gtttatgatt atcacagcgg cgcaccactc agccctgccg
     3001 atgtaaaaaa gattgagagt gagtttaaaa atgacagaac gaagatcggg aagatctcat
     3061 ttacagaaga ctggtactat ttcccggagg acaacagggt tgaaaaaagg gcaaagtctg
     3121 tcactttcgg ttatgagcta tacaacaaca cggggaaggt ttatgcatac agggctgctt
     3181 tccgggctga cctgaactga caaacgacaa accaaaacat cttcagtatg ccaagttatg
     3241 atatcaccag tctgaatgac tttatcatca gcaaacagaa agactttccc ttcgcacagg
     3301 gggagttgtc aagccttctt catcacatcg gcacagctgc caagatggtc aacgccaaga
     3361 tccgcaaagc cggactggta gagatactgg gcagggccgg agctgaaaac ccgcagggcg
     3421 aggaacagca gaagcttgac cgttatgctg atgaggtatt catcgatgcc cttaaatcgt
     3481 caggccagtg tgcgggtatc gcctccgagg agaaccagaa cgaggtcgtc ttcaccgggg
     3541 agtttgcttc ccggggcaag tatgtggtct gtatcgaccc gctcgacggt tcatccaaca
     3601 ttgacgtaaa cgtatcggtc ggatcaatct tttccattta ccgccgcgtc actgaacgtg
     3661 atgaggaggt ctcaatcgca gatttcctcc aggagggaag caggcaggtt gctgccggct
     3721 atgttatcta cgggtcatcc accatgctgg tctacaccac cggcaacggg gttaatggct
     3781 ttacccttga ccctaccatc ggggagttct gtctttcgca cccgaacatg aagacgccgg
     3841 aaacaggaag gatttattca atcaatgaag gtaactatat caagttccct gaaggggtaa
     3901 agaaatatat caaatactgt caggagaatg atcctgccac caaacggcct tacatctcaa
     3961 ggtatatcgg ctccctggtc tccgatgtgc atcgcaacat gctgaccggg ggcatctttc
     4021 tttatccgcc ttcaatcagt tacccttccg ggaagctgag gctggtttac gaatgcaatc
     4081 cgatctcgtt cattatcgaa caggccggcg gcgttgccac cgacggcacg aggagaatcc
     4141 tttcgctgaa acccgaaagc cttcatcagc ggactccatt ctactgcgga tcacctgata
     4201 tgatcaggaa gcttgtggga atgtgctgct gaaagttcat cgctttcttt ttgcggtgag
     4261 cttcggcttt gttaattttg caccctcaga caaaatcaat ggccagcacc gttttcaggg
     4321 acatttacag gcagcatcca tcattccgca ggcttgagga attgctggct ggtgacagcg
     4381 cagaaccggt gacgatacac gggctgacag gctcgtcacg ttcaattgtg atggccgggg
     4441 cactgtcaca gcagcccacg acacacgtgg tgttgcttcc cgaaaaggaa gacgctgcct
     4501 acttctacaa tgacctcaca tcactcatgg gtgatgacaa catcttcttc ttcccgggaa
     4561 cttacaaaag gtcagtgatg tatgaccaga ctgagcccgc caacatcatt ctcagggctg
     4621 aggtactcaa tttcctcagc tcggggcgaa gaaaatgcat catcgtcacc taccccgaat
     4681 cggtcatgga gaaggtcatc agcaaatcaa gccttaagaa aaacaccttc ttcataaaaa
     4741 aaggagcgaa actctccatc gagttccttg aggaggtcct ccgtgaatat aacttccggc
     4801 gtactgattt tgtctatgag cctggccagt attcagtcag gggaagcatc accgaccttt
     4861 tttcctattc agcggacaaa ccttacagga tagacttctt cggcgaggaa gttgattcaa
     4921 ttcgttcgtt cagtccggat gaccagcttt ccctgcagct tcatgatgag atacaggtca
     4981 ttcctgacat ccagttgctt gccttccagg agagcgctga cagcttcatc gatttcattc
     5041 ccccgtcatc aatcatctgg agcgacaatt ttgactacat cgttgagaag actgacaaca
     5101 tatgggcgca ggcccggagc cgaagctcga agggagagat atccgaccgg cgtgacatta
     5161 tcatcacagg ccgtcacctc gaagaactct gctcgcgtca ccgccgtatc ttcttcggca
     5221 ggggaaggca ggaccctgat acagtcaaca tcgggttccg caccgagccg cagccggcat
     5281 tcaacaagaa cttcggcctg ctgacatcaa agcttgcctc aaacgttcag gagggctaca
     5341 gcaacttcat catctcagag agcgaggtcc aggaacagcg cctgaaggag atcttcgaag
     5401 agaccgatcc ggcagttcag ttcactcccc tcctgctcaa cctgcatcag ggctttacag
     5461 accatgacct taagatagcc gtttacactg atcatcagat ctttgaccgt tatcacaagt
     5521 tccgtatcag gggcttcttc actcgtcgtg acagcatgac cgtaagagag ctgactgatc
     5581 tcaaccctgg tgattatgtt gtacatacag accatggcat cggccggttt ggcgggctgg
     5641 agaagattga ggtcaacggc aggatgcagg aagccatcaa gctggtattc aaggataatg
     5701 acatattgta cgttggtatc cacgccctgc accgcatctc caagtacaag ggcaaggatg
     5761 acaccccgcc gcgcatcaac aagctgggca caggggcctg gcaaaaggta aaacaggcga
     5821 cgaagaagaa ggtaaaggac attgcccgcg acctgattct tctttacgca aagcgaaaag
     5881 cctccggcgg tttcgcctac tcgcccgact cataccttca gcgcgagctg gaagcctcat
     5941 tcatctggga ggatacgcct gaccagctag ccagcaccgt ggctgtgaag gaggatatgg
     6001 aggccccggt gccgatggac cgcctcatat gcggtgatgt gggattcggc aagaccgaga
     6061 tagcaatcag ggcggcattt aaggcagcca ctgacggtaa acagaccgcg gtgctggtac
     6121 ctacgaccat tctggcgctg caacatttca gaaccttttc gtcccggctt gagaacttcc
     6181 cctgcaaggt tgattacatc agcaggcacc gcaagcccgc ggaacagaag aaaatcctca
     6241 aacaactctc cgacggcgaa attgacatta tcgtgggaac ccacaaactc accggcaaag
     6301 aggtgatgtt caaggacctg ggtctgctcg tcatcgatga ggaacagcgc ttcggcgtag
     6361 ccgcgaaaga gaggctcaag acgctcagag ccggtgttga caccctcacg cttaccgcca
     6421 cacccatacc ccggacactg cagttttcgc tgatgggagc ccgtgacctg tcagtgatca
     6481 gcactcctcc ccccaacagg atgcccatca tcaccgagct tcacggcttc aatgaggata
     6541 ttatccgcga gggaatagtt tacgaggtta gccgtaacgg gcaggttttc ttcatcaaca
     6601 acaggataga aaacattatg gaggtgaaag cctccctgct ccacttctgt ccggagataa
     6661 agattgccgt tgtacatggc cgcatggagg gtgatgagat agagaacgtc atgtctgatt
     6721 tcatacgcgg cgattatgat gtgcttctgg caacaacaat cattgaatcg ggcctggata
     6781 tccccaatgc caataccatt ttcatcaatg atgctcataa tttcggcctt tccgagcttc
     6841 atcagcttcg tggcagggtg ggccgttcca acaggaaggc cttctgttac ctgctcactc
     6901 ccccgctctc ggtcctgacc ggtgacgcaa ggcgcaggct caaggccata gaggagaact
     6961 atgagcttgg cagcggattc aatatcgcac tgcaggacct tgacatacgc ggtgcaggga
     7021 acctcctcgg aggtgaacag agcggcttta tagccgacgt gggttatgaa acctaccaga
     7081 agattcttga cgaggctgtc agggagctga aggatgagga gttcggcggc ctcttccccg
     7141 ggccggcgga gaagaaggag cacgctgccg aggaaaaacc gaaacaattt gttgaggatg
     7201 tgacggtaga gactgacctc gagataatgt tccccgatga ttacgtctcc aacatacctg
     7261 agcgaatcag gctctacaag gagctcaatg atatcaggga tgaggcaggg cttaaagcct
     7321 atgagtcaca ccttacggac aggttcgggc cgcctccgcc acctgcgctg gcgctgctgc
     7381 agctggtcag gctcaaatgg gcagcagccc ggacaggaat tgaaaaagta attcttaaaa
     7441 ataacacact gattgctaac tttgtgacgg accagtcgtc gcagttctat cgttcgccac
     7501 tgttcgtgtc ggtgatgaac tatgtcaaca ggagacaggg caacatgtca gtgaaacaga
     7561 acaacaacaa gctgagcctg acggtcagga gtgtcaccac cgtggtgcag gccattgacc
     7621 ttttcacagc gatactcgca tggcatgagt cggctcaggc ttcaaaggca aattaactga
     7681 tgaacctgat aattgacatc ggaaacactt cagtcaaggc agcgcttgtc agcaagggtg
     7741 tgataataag ccaggaacgg tttggcagta ctgatccgga gatgattggc atgttcacgg
     7801 ggcgtcgcag gctttccggt gcaattgtct cgagtgttgg cggtgatccg ttggggctgc
     7861 ttgactggct tcgcaccagg gccgggaggg tacatcacct cacctcacag tcacaatacc
     7921 cgttcgcaat agactacgcg acaccggaga ctctcggcac tgaccgtctc gccgccgcag
     7981 caggggctgt tgatcatcat ccgggagctg acctgctggt gattgatgcc ggcagcgccc
     8041 tgacagttga ccaggtgacc ggcggagttt acaggggagg aagcatctca ccgggattgg
     8101 caatgcggtt ccgggcgctt catgaattca cggggagact gcccctggtg acaaagagaa
     8161 ccgggttcac cttcccgggc aaaacgacgg aagatgctat tgctggcggt gttataacag
     8221 gcctggtgta tgaaataatt gagtatattc gcacattcga ggaaaaacac cataattccg
     8281 ttgcggtcat cacgggcgga gacagcggtc tggtcacgcc gctggcagga ggcagattgc
     8341 ttcactaccc tgacctggta acggagggat tgaacttttt actggaaaca aatgtataag
     8401 ggcataagag caattatgat aagtctcctt gctctcctgg ctacaggagc tgcaggtcag
     8461 aaaacagtca atacaccttt cgcgaggtac ggaatcggca ccctggagct tcagggagct
     8521 ttcagaacgc gggccatggg gggcatcagc agcggcatca gggacaacct cacgctgaac
     8581 taccttacgc ctgcgtcgta cagcagcact gacacaacct ccttcctttt cgacttcggc
     8641 attgattacg ccgtggcaaa gctgaaagga gatgacctca gatacaattc ccaggacatg
     8701 accttctctc atcttatgat gggatttccg ataatgaagg gctggggcgt tgctgctgcg
     8761 gtattgcctt atgccaacgg atcatatgat atcggataca agtcagaagg caccggagtg
     8821 accggctcca tttacgaact gcataacggc agcggcggat tccagaaggc tcttcttggc
     8881 acaggagtcc gggtccttcc cttcgtctcg gtcggcgcta atgtcttcta catgttcggg
     8941 gaggagacca ggatcaatga ctttgtgttc acaggtgaca acaattatta taataccagg
     9001 aagcagggta catcaggaat gaggggattc ggttacgatg catcggtaca gttcatgctc
     9061 aatctttcag ggggcaggtt catcaatgcc gggctgacct acacgcctgc atatgacctg
     9121 aagacaaaca acgaggatat tataatgaga tactcgacgg tctccaactc catatattca
     9181 tttgacaccc ttacctacgg caaactctca accgccagcc ggatgccaca gtcactcagg
     9241 gcaggcttct cactcggcaa ggttgacaag ctgacggccg gggcagacat tgtatataca
     9301 cgctggacca aagcatcact gccggggacc tatggaacct atactgatgc acttgcactc
     9361 catgccgggg cagaatacat acctgagaga tattcaaatt acagtttttt tgaccgtgtt
     9421 gaataccgca taggatgccg ctatggtgag agttacaccc tgtttggcgg agatgagctg
     9481 aaagagtacg gcataacttt tggaacaggt ataccaatga aaagatcgag atcacgaatc
     9541 tcactgacgg tagacctgtc aaccagggga gggaacggga gtataccgca ggaaacaagg
     9601 gtatcggtcg gagccagcct gaatctgtat gattactggt tcgttaaaag gcagtatgat
     9661 taaaaaatga ataagaaaga gatgaaaaga acaaccagca cgattttcac cggattgctt
     9721 ctgtcagtgt tttcattggc cctccaggcc cagaagggtg ttgaaacggg cacaccgttc
     9781 ggaagaggcg aggacagcac gaggtgcctg cgcaatacat ccctctattc aacctactat
     9841 gagaacaagg actacaacat ggctgttcag ttctggagac cggttttcta tgagtgtccc
     9901 ggatcatcaa agaacactta cattaagggt gagacaatgt tcaaggagtt cttccgcaag
     9961 acgggaaaca aggcttacat tgacactgta atgatgatcc ttgaccagcg gaccaaatat
    10021 ttcaacgaag agcccgcaaa caacctccga aaggcatttg ccctttacga gttcgggggg
    10081 aatgatgccg actacgccag gaggtgctat gatatcatca agggtgtgat ggagacatcg
    10141 ccccagagct ttgaccatac ctactcaagt ctctatatgg ccatcacggc aaaatgctat
    10201 gccctcaagc ttattgaggc gcctgaggtt atcactgcgt actcacagtc aatgaaggtg
    10261 gttgacaacc agctttcacg caaacccggt gacgcacgct ttactgaagc caggaagaac
    10321 attgacgcag tcttccggtc cagcggcgcc gcctcatgtg agaaccttga gcatctcttc
    10381 accgccggtg ttgataacaa ccccggtgac accgccatgc tcaggaaggt gctcaccctg
    10441 ctcggtgaaa ccggatgcaa ggactcggag ctttactaca aggcagccac caatctttac
    10501 cgctctgatc cgagtgcatc atcagcggct cagctggctg aaatgaacct ggcccgcaag
    10561 aaatacaacg aggccgaaaa atactacact gaagccattg acctggagac tgacagcaaa
    10621 gtcaaatcag gactgcttac caaactggcc acccttgagc tgacgtcaga cagcaagcag
    10681 gctgcgcgtg actttgcaag agctgcattc tcgctcgatc ccaccaacgg caatgccctt
    10741 ttcatcatcg ctgaagcata tgccgggtca cggattggcg aaacattcga gaaccagacc
    10801 gtttactggg tggtggttga ctacctcatc agggcaagaa acactgatcc cacccttaag
    10861 gaacaggttg acgagcggat tggaatatat tcaaggctat tccccacaaa ggaggaagcc
    10921 ttcttcagaa gcctcgtcga ggagggcgct tcctaccagg tgggcggctg gatcaatgaa
    10981 tccacaacca tcaggttcag gaaggaataa cagcagtgaa gtccttcaca acatacaata
    11041 tcatgatagc agtcctggtt gccgggactg ctttgctttc atcgtgtgac aagaaggaaa
    11101 caacggtaaa ggatctgatg ataaaggggt tgccgacgct ttcgtcaagg aatgttgaaa
    11161 ccatgtattc tgactccggt aaagtcacca tggtggtacg cacacccctg atacagcagt
    11221 tcacctcaga agaaaacccg tataccctct tccccgaagg gctgacagtg ttgttctatg
    11281 accggactga cgtacctcag gcaagcatca cctctcacta tgcccgctac acggagaagg
    11341 atgatgtatg ggaactccgt gacagtgtgg tggctgtgag caacaaggga gaagtgctgg
    11401 agacggaact gcttttctgg agtcagccca gggacaggat ctggtctgac aggtttgtca
    11461 ggttcacaca taatgaccag attgtgatgg gtacaggatt tgattcagac tcacgctttt
    11521 ccaactggac aatcaagaac gtaacaggaa caatttatat cagggatgaa caagaagtca
    11581 ctcctgctga ttgagatcat ctggatcatt ctgggactga tgtgccttgg aatagccatc
    11641 cgtgagacag tcaacgccgg cataggccgg gcatggctgt tttatgtgat ggccatcgca
    11701 gcatttgttc tggcgtggat acgtgactct cagcgaaaaa agctgtaaat ttggggcatg
    11761 aacccatggg tcataatccc ctttgcaatt cttcttgccg cattcttttc ggcaatggag
    11821 cttgcattca ttgcctccaa caagcttcgt attgagcttg accgcaagca ggggaatttc
    11881 ggatcgggca taatcgccct cttctcacgc aacccgggcc tgtatatatc aaccatgctg
    11941 atcggggtca acattgccac ggtcatattc agtatgcttg ccgctgacct gatggaatca
    12001 tggtttaaaa acctgatccc ctcagaggtg ctgatcttca tcatccagac agtaatcaca
    12061 acaatggtta ttcttgtgtt cgccgacttc ctccccaagt cagtggtgcg gatcagcccg
    12121 aatttcttcc ttcgcatctt tgctgtcccc acagccatat tctattacct cttctacccg
    12181 gtcagcaagt tcacgctctg gctggcaaac atgatcatga ggattttctt cggtataaag
    12241 acagagagga aagagcagga aaacaggatc ttcacccgga ttgaccttga ccatttcgtc
    12301 aatgacatgg ttgagacgga tgaggagaag gaggaggaaa acagcagcct caggattttt
    12361 cagaacgcac ttgatttttc atcggtaaag gcacgcgagt gcatggtgcc aaggacagag
    12421 atagtggccc tgccggtgca ctcctcacct gacgaattaa ggcaccagtt catcgaatca
    12481 ggccactcca agatcctact ctaccgtgac aacattgaca acatggtagg gtattacgag
    12541 ctgaaggaca tactgcacgg gccggctgac attgccaccg gcatgcgtaa gctgcctgtt
    12601 gtccctgaga ccatgaccgc caacagggtg ctgaggttgc tctctgatgc caagaagagc
    12661 attgcacttg ttgttgacga gttcggcggc accgcaggag tgatcaccat cgaggatgtt
    12721 ctagaagaga tagttggtga cattgaagat gagcatgaca tcagtgacct cgtggagaag
    12781 aagataaatg agaaagagta tgtcctgtca ggacgccttg acatagacta cctcaacgag
    12841 gagtacgacc tcggcctgcc ggagagcgat gagtacacca cgctggcagg cctgatactg
    12901 tttgctcacg gcaacatccc gcgccagcag gaaaaaatca gggtagggcg taatcttttc
    12961 acggtgctca ggagttcctc aacaaaggtg gaactgctga agctccagat tgactgagag
    13021 gtctgtgcaa acgggcacac ggcccggcca gacacgcagg caggcgggcg aacaagcggg
    13081 cgggcgcgcg aacaggcggg cataatggca gccacgcaga cggtcacgca gaaagtgatg
    13141 accggtaata ccggtcagat tatcaacctt tctttttcag ttcagcgtaa tacttgaaat
    13201 aaagaggaat tgccctcatc cctgcaaaca ggttctcaag cgggtaattc tcattgggtg
    13261 agtgtattgc atctgactcc agcccgaatc ccatcagaat cgattttacc ccgagtatct
    13321 tttcgaaggt agaaatgatc ggaatgctgc cgccgctcct tatgggtatt ggcttcctgc
    13381 cgaaaatttc agtataggcc ttctcggctg caaggtattc cggggtatcg agtgggctca
    13441 catacgcctc acctccgtgg aggtattcgg cgctgacttt tactccggca ggcgccagcg
    13501 acttaatgtg gttgatagcc agttcagcta tatcaccggc tttctggtat ggcacgagtc
    13561 tcatcgagat ctttgccgag gctttggcgg gcaggatggt cttggccccc tcgccggtat
    13621 aaccgcccca gatgccgttg acgtcaaggg tgggcctgat acctgtactc tccagtgttg
    13681 agaatccctt ctccacagcc agggcatcaa cgcctacgga tttcctgtat ttttcaggat
    13741 caaacggtct ctcagccatc ttggctctct cggcatcaga gggaacatgc acgttgtcat
    13801 agaagcccgg gattgtcacc ctgccgtcgc tgtctttcag cctggcgatg atatcacaca
    13861 gagcattgca cgggtttacc actgctcctc catagatccc tgaatggaga tcatgatccg
    13921 gaccctcaac ctctacctgg atataagaca atccgcgcaa tcctgatgtg attgacggta
    13981 tctcaggtga gatcattgtg gtatctgaaa ccaggatcac atcagagcgg agcgtctcgc
    14041 tgtattcagc acagaatccg gcgatgctcg ccgaaccggc ttcctcctca ccctcaatca
    14101 tgaacttgac gttgcatgca agggtgcctg tggcgttcat catctcgaat gccttcaggt
    14161 gcatgaagag ctgtcctttg tcgtcatcgg cgccgcgtgc ccatatcttt ccgtcacgaa
    14221 tctccggctc gaagggtttc gaggtccact tctcaagagg ttcagcaggc attacgtcat
    14281 aatggccgta gacaagtatc gttggcaggg acggatcaac catcttctca ccgtatacga
    14341 tgggattgcc gggggtggca tagactgttg cctggtccgc tccgttggca gccatcaggt
    14401 cacgtatgta ttcagcagct cttaccatat cggccttgct ggattcagaa gcgctgaccg
    14461 aaggtatcct gatcagtccg aatagttcct cgatgaatct gtccctgttt gtttcgatgt
    14521 agttcttaat gctttccatt atcgttgtat ttatgatatt cgccggccgc ccagtgcagc
    14581 ctgttgtacc actcttcgcc aaaagccctt atcaggggtt cgcgaaggaa gaggtaaagt
    14641 tcagtattct gtttcgctcc gcaggcgaca ccggatttgc agataaacca ctcctcgtag
    14701 ttcactgccc tgaaagtgct gtactgctta accctcacgg ggaagaggtg acatgacagg
    14761 ggtttgcgga agtccacggc tcctgcgtta aaagccgttt cgatgccgca cttgtaaaca
    14821 tcatcatcca tgatggtgta tgcgcattcc tcgttggtga tcagcggtgt gaccagttca
    14881 ccttcgatgt cggtgatgct tgtgccctgc ttttctattg ccctgatgcc ttcagggcgg
    14941 agccatggcc acaggtcagg ccatatccgc tccagcgctg atgcttcctc tgccgtcagc
    15001 ggagcaccgg tgtcaccgta cctgcaacag cttcccttgc aggccgccag gtcacacaca
    15061 aacttcttct ctatgagagc cagggagact atggtgtcat caatctgcag catcgtttcc
    15121 ctcacacaag tgatttgcct gtcatctccg ggggaacggg tatgcccatc attgtcagaa
    15181 tggtgggcgc cacgtcagcg aggatgcctg ccctgacgct tttgtacctt ttgcttataa
    15241 ggtaaagggg gaccggattg agtgaatggg cggtattggg tgatccgtca ggattgaccg
    15301 cataatcagc attgccgtga tcagcaatta tgatcacctc atagccgttc gctgttgcag
    15361 cctctgcgac atcaccggca cattcgtcaa cagttcttac ggctttctga atggcttcgt
    15421 atatcccggt atgaccgacc atatcaccat ttgcaaaatt caggcagatg aagtcaaatt
    15481 tctttttgcg gagttctgca ataattgcat cccttacccc gtaagcgctc atctcgggct
    15541 gcaggtcata ggtggccact ttcggtgaag ggatgaggat ccgttcctca ccctggaact
    15601 ccttctccct tccgccggaa aagaagaagg tcacatgggc atatttctcc gtctcggcaa
    15661 tgcgaagctg tttcttacct gcatcagcaa tgatttctcc gagggttctg tcgacattct
    15721 ccttggggaa gatgacgtga agtccgcgga acttatcgtc atacggggtc atggtgagat
    15781 agtgcagggg aatggtcttc atcccggcct ccggcatgtc ttgctgcgta agcactattg
    15841 tcagctctcg ggccctgtca ttcctgaaat tgatgaagac aaccacatcg ccttctgcga
    15901 tagttgccag gggatgtccg tcagcgccgg ttaccacgac aggtttcatg aattcatctg
    15961 tgactccggc atcatatgac tcctgcattg cctggagtat atccctggtc tgtgttcccc
    16021 tgccgtggac aagcatctca tatgcctccc tgatccgttc ccatcgcttg tcacggtcca
    16081 tggcatagta acggcctaca aaagatgcca ccctgccgtt gcttttttcc agatgcttaa
    16141 gcaggtttgc catgtaaccc tttccgctct tcgggtcagt gtcacggccg tcgccgaagc
    16201 catggatata aacctccttc aggccataat ccatggtcat gtcacaaagt gcatagagat
    16261 gactgtcgag cgaatgaacc ccgccgtcag agagcagtcc catgaagtga accttgaccc
    16321 ctttttcgcg ggcataggtg aatgcctcgc ggagagtccc gttcgtgcgt atctcgcctg
    16381 tgcggcatgc tttgtttatc ttaaccaggt cctggtagac aatccgtccg gctcctatat
    16441 tcaggtgacc gacctccgag ttgcccatct gtccttcggg gagacccacg tcttcgccgg
    16501 aggtgagcag ttgagacgag gggaaggcct tcttcagggc ttcaatgtgt ggtgcgccgg
    16561 ttgtgctgat cacgtcgctt ctggagccgt caccaatccc ccagccgtcg aggatcatca
    16621 gtagtatttt gttttctttc atcaaatcaa taattatttc atatgctggc agcaaaaata
    16681 gccatttccg gcaagattgc catcgcggta ctataacacg gcagaggctt taaagtttga
    16741 atcagcgggc tcccctgatc tccctgaccc tgatgcggta ttctttatcg gggcaggtgg
    16801 tctcgtactc tatatccctt cccacataag ctgaccattc ctgcatggtg agatttctcg
    16861 tgaccttgtc acagaggccg tcagtcatga gcctgatgtg tgcggggcgc ctgatgacat
    16921 ctgtctgtgt ggcagcaagg aatgactctc cgccgtcact gaaggcaagg cgcagaactt
    16981 cctgccctcc gtcggtaaaa actacgggag gctgctccag gttggccatc gtccatagcc
    17041 tgacttctcc tgcctcatca gctgtaagca tctgcccgtc agccacgtcg aaggcaatgc
    17101 cccttactgc tgacatatgt cctgacgcat ttccgtcagc aagaccggta gaggtgctcc
    17161 agatatcaat agtgccggta tcgtcccccg ttgccagcct gtttcctccc ggtatgaatt
    17221 tatgagcggt tatgatttta tctcccgtat tgatggtgat atgcttcgca ggcgaccctg
    17281 tttgccagca aatcagcctg ccgttgcccg tcagggctgc cagcatgtta ttgtcatccg
    17341 aaatttccag agcgactatt cctgaaccat cactggcaac ctttctcaca ctgcggtttc
    17401 tgagatccca ttcgttcaca ctgccccctg aggtggaaga gtaaagatgg tcagtgtctg
    17461 tatatgacag ggctgttatg gtgccggtgg agtcatgcaa ctggtaacct atcgaatcat
    17521 cgtcaaatgg tatcataagg atttccgaat catccgttcc gcaggcgagc catgatctgt
    17581 cagggctgag agtcatggcg ctgatggttt tgtttccgga ccataccagg tcatatcctg
    17641 cagatggttg ccctgctttc cagctaagca ccctgccttt ggaatcagcg gtaaagaata
    17701 tgccgtcaaa gccttcggcc atagcggtat actcagctcc gtcaggggta tacctggcac
    17761 agaacctgtt tccataacgt tttgaaacat cgtagagacc ggcaaagaca tctgcatcct
    17821 cagctatgcc atcatacttc cggttaaaga gataagcctg ccatgccagc aatatctgaa
    17881 ggtccttgtt cccggcatgg ttcagtgacc tcacagccag cgacctggcc agagagagca
    17941 tccggttgct gttagccttc gaaaatctct ccatgtcttc ggccttgccg gaagccatct
    18001 cccttaccgc ctcctcggct gatcttgccc gtgcttcagc ctgtttcacc ctctcctcag
    18061 gcatgatcag gttgccttct gtcgcagcgg gagctaatcc cagggctgac gacagtacct
    18121 ccaggctgtc actgagcctg tcgttcaggg taacagcctc gtctttcatc tggatggcaa
    18181 ccgatttctg cctttcagcc ctgttcctga gcgaataaac agcccccagg gcaaccaggg
    18241 taagaatggc caataaaccg agcaatcctg ctatcaggcg tgaacgcctg accgtttcat
    18301 tactgctttt ccgcccgtat tcttcccgta tcagatattc ttcctcagaa tttttcagga
    18361 agatgagggc tctttcgaag gcaggatcaa tcttttcggc ccaggcgagg gtgggattgt
    18421 tttcatctct ccacctgagg gccatcagca ggtcaggagc tgtccaaaga cggcctgtac
    18481 cctcctggta ttgtgcagct gcggaggcca gctgcctgta aagcttcact gatgcttcct
    18541 cttcatccat ccattttcgc agattccccc atagccttat gatgctttca tgggtcaggt
    18601 caaggatgct ttcgccgttt agcggcacct cctttgacgg ggtcaggaat gaatattccg
    18661 gtgatctgaa cacctcagcc acaccaatga tttcatgctc agcgcaccct gtaagaaccg
    18721 caattgttga aatacgctca ggtttcctaa gttccctgcc gtcatccgag cgtgtggtga
    18781 ttgtgcggaa gagccgggaa cagacataac ggtgcctctc atccagtgaa tcaaaggcct
    18841 gcccggcatg ctgagagatg gctcccttaa gcatcccgac agcttcatag tcagcgatac
    18901 cgatggggcg gctcaggtca ccaagcctgc tccactggtt ccaggtacgc atcatcaggt
    18961 gctgcaatac cggcagctgt ccgggtctct cttcaagatc actcagtata agcttcacca
    19021 gcgacctgtc aattgaacct cctgaaagct tcacaggctc ttcaataaca gtaaccatct
    19081 ggtcgacggg aatctgtggc agaaggtaac tgctgctgtt catcaggtct gtaagagaat
    19141 gaaaccgccc gcagtcagag acatattcag atctgagtgt caaaatgatg tacagcccat
    19201 ggtcgggttt ctgcatcgca ttgactatca ggtcaatgaa atctgtggca tcatcgccgg
    19261 gaaggcctct ggaagttgcc gggctgaacc ggaagatctc ttcaaactgg tcgatgacta
    19321 tcaccacctg ctgacggagg ttcttcctga tacggttgat cacgtcattc agtccttcgg
    19381 ttctgttatg cagtgatgcc gccacgctca catctgacag ctggttgaac cctgctcctg
    19441 cagagagtac tgacaattct gttgccaggt ggtcaaccgg attgagtgca ggtctgaaga
    19501 caagatatga ccaggatttt ttttcttctg tattttcctt cagaagagca ggaatgactc
    19561 ctgacagaac aagggatgat ttgccgctgc ctgatgcacc caatacagca acgaacctgt
    19621 tttccttaag cttggaaaca accttgagag tgctttccat tcgcccgaaa aacaagtgac
    19681 cttcgtcagg tctgaatgct ctcagtcccg gaaatggatt tggtctgata attatattcc
    19741 tttctggcat agaaaacatt tttagattgc aggtaccccc tcctggctgt ctgtataatt
    19801 tacaaagata tcagtgaatt ccggatagta aaacacaaag gcggcaatat catttaccgg
    19861 tattgaataa aggccggtct tcccgtcccg ggagggtacc acccatgaaa tcacccctgg
    19921 aagggaacct gcatcatatg accctgagta acggaggtgc gaggccagca tgatcatggt
    19981 ttcttcagga attttagaaa agcacaggct cagaaacctt gttttctcga agctcattgc
    20041 tgcctgcgga agggtgccac ccgtgactgc tgatattctt gccctcgccg ggtcagggag
    20101 acgcagctct gcatccctgt accagtcagg gttgattgac ctgatgacgc gggcgctctc
    20161 ctcctgcagt atctggctgt tactgaacag gtatgagatt gcatgttccc tgtcgaggcc
    20221 tctgccttcc gcagctgcca ggtggagcgc gcacgccttg ctccaggtgc cggtgatatt
    20281 ctgctccgaa gccaggagga atgatgaaat tgactggcct ttcacgctcc tgacgggaaa
    20341 gtaaagggaa agttcagcca gcctgcgatt gtcagtactg tttcccagaa gtgccttaag
    20401 cggtctgcgc attggttcgc ttatcacgct ttctattgcc tccgaggcga caccggcctg
    20461 gcatggcctg tcatcccgtt tgcgcggcag tatcaattcg gcagccactc ctcctgcaag
    20521 gagatgaaca agacacctga tgagttcata gttattttcc ctctcctgtt caagggctgc
    20581 tgacaggagg aaatgcctgg atcttgatac ctcagtctgc atggcgataa gcctggccat
    20641 gacatgaatg gtttcgctga gtgtttcccc gatcttaagc ctttgccttc cctgcggtga
    20701 ccatcctctg ttgcacaggg aagaggctgc cttaagacgg acgcccatat gaccggcaac
    20761 gacaaaatcg ctgagccacg ggaatgcctc gctggccggc atggcatcca gaagccggag
    20821 gataatgtaa ttctcccgtt cactgttgcc gggcctgatt actgttccga tcaggtcacc
    20881 gtatacctcc ggaccgaact gtctgagcac ataataggct tcgcgggcag tatccgggtt
    20941 gtcaagtccc ttcatcacct catcccgaag cgatgtcatg ccgtaacggc ctgccgccat
    21001 caaacccgtt ctccttactt cagcactggc atctctgagg agcttgataa tctcctgctc
    21061 cgaaggtttg cttttactta aaaatattgc cctggcagcg gcggctttct ccgggatacc
    21121 cgggaggcgt aaaaaatcaa cattcctgac cgggtcagaa taatactttt caatgactga
    21181 aaccccgttg gcagcttcat gtctcaactt gtcgctgaaa cggctggcgg aggcaatggc
    21241 cttcatggcc ggaagaagtg ccgggtcata agttttggaa gcaagtgaaa tgacttccat
    21301 ggcatagaaa tcacccgcat tgtttaacag gtactcaagc ctgccgccaa tcaggtcagg
    21361 gtaatggtca gcaacgagca tcacgctcat gaagccctct gacttctccg tcgcctgatt
    21421 ctgccctgct ccggaggttt ccgcaggtac cggggcatcc agcatcatgg caagcctgtt
    21481 ccggaagtga gccacgatga caatgtcaag aagcatcagc gcaatcgcag ccaccgggaa
    21541 aaggaagata atgccggaaa ttctcaaatc tgcagttatt gttaccacaa ctaaaatgac
    21601 ggcaatgaat gcggcagcaa tgaccgtctg cctggcaatg tcctctgcct ttatcctgcc
    21661 tgatggcagc cctgcctttt cagccaggtg actcacggat gccagcgctg gcagagtgat
    21721 cattgccatc gatggaagga agaaggcggc tgcaactgat gtgaccagtg ctgacagcag
    21781 cagcagagac tccctgccgg tgaggtcata ataaatatat atcagggcgg ccggaatggt
    21841 cagtgccagc agcaccatcg gggccagcag cagcttgccg cgaagaacaa ggttacgaag
    21901 tatgggcagg tagtaacggt ttctgattac cagtcctgtg ctgatgacta ttaccgagag
    21961 agtcagagtg tagaggagcg gtgtagcacg gaagagggac aggtcagtca gcactgcaac
    22021 catagctgca gcggttagtg ccatcaggac taatgccgta aggaagaccc agttgaaacc
    22081 ggttttcata gccctccgag gttttgacat atccgggttt aagtacagac tttcttaata
    22141 aagatacggg gaaaaaatga aacctacaag taggctccct ttgatcttac agtgccagca
    22201 atcatcgcag tcatctcttc ctctcccatg gtctccgaat ccaggatgag gtcagcctgc
    22261 tcataccatg agcttctttt tcccatgagc ttctgcacga ttgaatgaag ttcatcaggc
    22321 ccggcatctt tcagcagcgg cctgtcggtc ctggaccggc tcaggcgtga caccagggca
    22381 tcgacggtca gtctcaggta tatggttact ccggttgcct tcatgaggga aatgttttta
    22441 tcagaacatg gggtgcctcc tccgcaggct acaactgtcc tggtccgtcc tgatacatct
    22501 tgcagcgctt ctgcctcagc cttcctgaaa tattcttcac cctttgtggc aaaaatgccg
    22561 gctaccgtca tcccctcttt ctcctctaca agtttgtcaa ggtcagtgaa cctccatcgg
    22621 agaagcgcag caatgccctt ccccgcggag ctttttccgc tgcccatgaa tcctatcaga
    22681 taaatattca cggataccta aagatctagt ttcatctggg ccggatcatc aggcgtgtga
    22741 tcctgtagcg gggactcatc cgcagtaccg ttctccggtt ccggaggcac aggcattgcc
    22801 tcgcttctga ccacgggatc aatctccctg atatcggcaa cactgtagtt ggaaagcctt
    22861 cttcccttgg cgcggaaact ctttacaccg atgaattccg ccacctctat tatctcctgc
    22921 ttgcggcctt cgttcttgcc gccaaacttt atctcaaacc tagggtactc cacctctgtg
    22981 aggctcacca gccgtgatcc ctcactgtca gagatgaaac actgaagcct atctgcctcc
    23041 tcaaaggcaa accgtttgac atagtaaaac tgttgctcct catcccagta tacagaggag
    23101 aagatctttg actcgcggaa tttttcaagc agcaacaggt catcctcgaa atgtgatgag
    23161 aggtcagtgc cggaggtccg gtaccaaccg ccccgggtga ccacgagcag catctcttcg
    23221 gaagcgaact cacccaggaa tttccccctg ccatcggcat tcagcctcat caccgcatca
    23281 tcaaaccaca gtttgcggcc gccaagggtc gagaggccct tttccttaag ggtgatctta
    23341 tgaaccgcgt tctttgtcag gatatttccc attgaaccac gtcccttgat tgccagctcc
    23401 ccgaagtcaa aatcaaaggt cagcttcttc agcctggcct tgggtttatg gtacaccctt
    23461 atcacctcag cctcaccgtt gggattggcg ctgagataca ggatcctagt gccggctgta
    23521 cctcttccga ggttgtattc cttgtcgcgc gtgagccctg taagtgcaca gcgtttgaca
    23581 taataaggcc cttcccggcc atcctggtat gccacattgt aaatggtgcg cgaatcattc
    23641 ctgagaaata cctgtgcgtg aatgacatcc tttccgacaa actgcttttc tgcaaccttc
    23701 gtgataagat aggtgccatc cttcctgatg actatgatat catcaatgtc agaacaatcg
    23761 cacacgtact catccttttt aagccccgtt ccgatgaatc cctcctggcg gttcacgtaa
    23821 agcttggcat tggcggccac caccttggtc gcctcaatgg tctcgaagct acgtatctca
    23881 gtcctgcgct ccctgccctt gccgtacttt ttcctgatct gacgatagta gttgatggca
    23941 tatggaatga tgttggcaag atggttggcg acttcttcca ggtctgtctc gaggcctctt
    24001 atatgttcat cggcacgcga ggaatcatat ttcgagatcc gcttgatctt tatctctgtg
    24061 agctttatga tgtcatcgcg ggtgatctcg cgcctgaaaa gctttttata tggcttcagc
    24121 cctttctcaa tggcggtgat cacagcctcc caggtctcgc actcctcgat gtcacggtaa
    24181 attctctttt cgatgaaaat cttttcaaga gacgagaagt gccactcttc ctccagctcg
    24241 gccttgcgta tctcaagctc cagcttgaga agctttaccg tctggtccgt attgtgcctg
    24301 aggatatcac tcacgccaac gaacgcaggc ttgttttcgt cagtgatgac gcatgcgttt
    24361 ggcgagattg acagttcaca gtctgtgaag gcatagagtg cgtcaatggt cttgtcaggc
    24421 gagatgccgc tctgcagctg aatgactatc tccactcccc ctgcggtgtt gtcatcgatc
    24481 tttttgatct tgatcttgcc cctgtcattg gccttgacta ttgtctcgat gagcgaggtc
    24541 gtggtgcgtc cgaaaggaat ctcggaaatg accagtccct ttttgtcaac cttctcgatc
    24601 ctggccctga ccttaactac ccctccgcgg gcaccgtcat tgtattttga cacatcaatc
    24661 atgccagctg tcgggaaatc agggtacagc tcgaagggtt tgccctgcag gtaattaatg
    24721 gaagcgtcaa tcagttcatt gaagttgtga gggagaatct ttgaagagag gcctacggcg
    24781 atgccttcca cgcccatagc aaggagcagc gggaacttca ccgggagggt aacgggttcc
    24841 ctgttacgcc cgtcatatga gctcatccac tcggtggtct tgctgttgaa ggtcacctcc
    24901 agggcgaact ttgagagcct ggcttctata tacctgggtg ctgcggcgcc gtcacctgta
    24961 agaatgtttc cccagtttcc ctgtgattcc acgagaagtt ccttctgtcc cagctgtacc
    25021 agggcatcgc ctatggaggc gtcaccgtga ggatgaaact gcatcgtatg cccgatgata
    25081 tttgcaacct tgttgaaccg cccgtcatcc atgcgcttca tggagtacag tatacgacgc
    25141 tgtaccggtt taagcccgtc agacatgtgg ggcacggctc gctcaaggat tacatatgac
    25201 gcataatcca ggaaccagtc ctggtacatg cctgagagag ccgtgacagt atgcagggcc
    25261 tcaggtatat aaccggtttt gggtacttcc ttctcccctc cctcttcttc ggggatcagt
    25321 tcaaaaggat cactttcttt catcatctgc tgaaaccttc actcacgcct gggctgcttc
    25381 cggaatttcc tcctcaaccc gcaggttgtc aatgataaag tcctgacgtt caggagtatt
    25441 cttgcccata taaaactcga ggaactgggt atacggatct gctttcttca tgatcacagg
    25501 ttccaggcgc atgtctttcc cgatgaagtg aataaactca tccggggata tctcgcccag
    25561 ccccttgaag cgggtgatct cggggtcagg accgagatcc tttattgctt tttgcctctc
    25621 ctcatcggag tagcagtaac gtgtcagttt cttgttgcgt accctgaaca ggggagtctg
    25681 caggatgtaa acatggccct tgcggacgag gtcagggaag aactgcagga agaaggtcag
    25741 gagcagcagt ctgatatgca tcccgtcaac gtctgcgtcg gtggctatca ccacatagtt
    25801 gtaacggagg tcatcgagag actcctcaat attgagggcg gcctgaagca gattgaactc
    25861 ctcgttctcg taaacaatct tttttgtcag gccgtagctg ttcagtggtt ttccccggag
    25921 gctgaagaca gcctgggttt ccacattgcg cgatttggtg atggagccgc ttgccgagtc
    25981 gccctcggtg atgaagaggg ttgattcgag ccggcgctca tcgtttgagt tgtaatggat
    26041 gcggcagtcg cgcagcttgc ggttatgcag gctcaccttt ttcgcgcgtt cacgcgccag
    26101 tttctgtatt gagctgatgg ccttccgctc cttctccgac tcctggatct ttttcagcat
    26161 gatacgggca gtctcaccgt tcttgtgcag gtaatcgtcg aggtgcttgc ggatgaagtc
    26221 aaccacatag ttacgcaccg aggggccgtt tggtcccata tcctttgatc ccagtttggt
    26281 cttggtctgc gattcgaaga tgggttcctc tatcttgacc gagacggccg cgatgattga
    26341 tgatcttata tcggaaacgt caaagtcctt tttgaagaac tccttgacag tgcgggttat
    26401 cgcttcgcgg aacgcaatga ggtgtgttcc tccctgtgag gtatgctggc cgtttacaaa
    26461 gctgtagtaa tcttctccgt actgggttcc gtgcgtcatg gccacctcaa tgtcatcacc
    26521 cttcaggtgg atgatggggt aaaggccttc gcagttcata ttgtcattga ggaggtcgac
    26581 gagaccgttg cgcgagacta tcttgttgcc gttgaggttg atgatcatac ccgcattgag
    26641 gtacgagtag tttttcatca tgctctcaac gtactcggct atgtaatgaa agttgccgaa
    26701 catggcttca tcaggcacga agatcacctc tgtgccgttc tcttcctcag tttttctcag
    26761 gggctcatcc cttaccagct ccccgcacct gaattcaatc tccttaagct ggctgtcacg
    26821 gaaagatctg ataacaaaag atgatgagag agcattgacg gctttgatac ccacgccgtt
    26881 gagacccaca gatttcttga atgcctttga gtcatatttg gctccggtat tcatcctgga
    26941 ggagacatcg agtacctttc cgagtggaat gccacggccg tagtcgcgga ccctcacttc
    27001 cgagccaacg atgctgacgt caattctgcg gccgaaaccc atcatatact cgtcaaccgc
    27061 gttatccatc acctccttga gcaggacata tataccgtca tcaagtgagg agccgtcacc
    27121 cagcttgccg atgtacatac cgggacgcag cctgatatgc tccttccact caagggtttt
    27181 aatgttctcc tcagaataat tgccgtccat tatcccaaat tttggggcaa atataaagaa
    27241 ttaggcaagg cgaatctttc cgatttatca acaaccaggg ggaattgtgc ccagatttgc
    27301 gaattccgat ttgcgatgtg cgacaaataa accaaaactc gcaaatctca aatctcaaat
    27361 ctcacatgct actcgtttcg cagctggtcc atggcggtaa ccatgatgga ctgtaccctt
    27421 gtggccagtt cctccgggtc gccggtgccg aactgccccg ggaagatggg atccaggacc
    27481 ctgatcctta caatgcggcg gttccggaaa atgattccac cttttttggg catgatctcg
    27541 cctgtgccgt caatcagcac gggcagcagc ggcttgtctg tcttcactgc aagctggaac
    27601 gcgcccgtct tgaactttcc gatagcgccc gtcagtgagc gtgtaccttc ggggaatatc
    27661 accacagaga ttccgtcatg cagtgactcc acacactttt ccatcatttt tacaacgctg
    27721 gatttgttac ccctgatcag ttcgatgtac ttgaccattt tcatctccca ccctaccaca
    27781 ggaacgcgga agatttcatc cttggatacc cacctcaggc ggtggcgcaa catgttgaaa
    27841 aagactatat ccagcagtga ctgatgattg gggatgataa tgtatgcctg cttctggtca
    27901 agcttctcgc gaccgtcaac tattactttc cagaaagggc tcatctttgt gagcacaatc
    27961 ccctggatga ccatccacct gttgttcatc agcctgcggc ggtcaaaggc aagtgcaagc
    28021 agccagagaa taactgcaac gggtaatgcg agcagtatga atgtgatgct cgagaaccaa
    28081 acccacagtg actttatcag gttcattgtt ttatgctttt attcatttt
//
LOCUS       DAOPXU010000030        26306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_1831_length_26306_cov_14.122319, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000030 DAOPXU010000000
VERSION     DAOPXU010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 26306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26306
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1831_length_26306_cov_14.122319"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PKK03_04650"
     CDS             <1..210
                     /locus_tag="PKK03_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HNX83717.1"
                     /translation="SRLAAESGTLLLGQIPIVESICDGGDSGRPVALGDNATGTAFMN
                     LAETVVRQVEIRNAEIAPTKRVDVK"
     gene            318..2975
                     /locus_tag="PKK03_04655"
     CDS             318..2975
                     /locus_tag="PKK03_04655"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX83718.1"
                     /translation="MRLRHREYTAAVMVIIALILAGCSAEKNTGASRFYNSLISKYNI
                     WFNGNEAYKAGVKKVRESHRDDYSDLLPVFEYSDAESARMASSDMERAVQKASKVIAL
                     HSITAKPEEKNRRNASAKSEEFYNRREFNEWVDDSYLLLGKAQFYQKNYMAARSSLSF
                     AVSIATDRDLVTEAGIWMARIQTEEGNYTEALRVLQETASPENLSRPLKEMYYSTRAD
                     IILRQKKYREALEPLTTATEYAGNNRTKVRLTYLLAQVCRAAGDNDLSTRYFSQVMKM
                     NPPYDLEFNAGINLAGVTDISHGDADDLSKSLRKMLRDSRNKDYLDQIYFALGELEQR
                     RGNTDEALKLWSQAAASSTVNTRQKARSYLAIAGHFYEKPDYLKSWQYYDSASYLIDN
                     RFPNYEMISRRTSDLGEYAGFHTVVITEDSLRRVAAMSTAERDALIAGVIRNVEAEMT
                     SQRTGDGSDRYNMGQYYENEQRYRGAISAEGGWYFYNQAALTFGRTEFRRRWGDRRLE
                     DNWRRTNKTRSAFAPGTQQEAENQKEKADSAGVAPERTKEYYLRNLPLTDSLLKISVK
                     RSAEALLGEGKILASRLNDTLSAATSLEEAARTGTDDNTRAEALYELYRLLSKTNPSR
                     ADRRRAELLASYPDSEYALILSDPDYANRHKEMAARASRSYDVAYLAFTEERFADAIS
                     LCTEAERLYPEDELLPKFMLLDAMATGALEGEMAYKAKLDSLVAKFPSTPEGQRAAEI
                     TDFLRKEKPEIRIAEDTRIAEEIYIADTLQPHYVIIIASNPRANMNQMVFDIINYNLD
                     VYPDRSYRTEGAAIDAGYLLITVGPFEKTADAVTWYRSFNPEQAVREAATAGLAVYLI
                     SRDNLQKFREDKNTDRYAIFHSKAYPNLR"
     gene            2991..4544
                     /locus_tag="PKK03_04660"
     CDS             2991..4544
                     /locus_tag="PKK03_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HNX83719.1"
                     /translation="MRQIRILWVDDEIGVLRSHILYLEEKGFETDTCHSGNDAIIMVR
                     EKAYDIIFLDEHMPGLSGIETLRRIKAIRPAIPVVMITKSEEEDIMDAAIGSQIADYL
                     IKPVKPQQILLTLKRILNIEEIVARQTTTVFREEFSSIADMISSASTFEQWCELYRRL
                     TFWQGELIRSADTGMREVLRMQESDANRAFAKYISGNYMKWVSAEAADRPLMSPAVMS
                     KRIFPMISKGTPLFFIIIDNMRLDQWQTIAGDLRQVMRTVDEELYLSILPTTTQYSRN
                     ALFAGLMPAAIEELMPEYWIDEHEDEGKNLNEEKLLAKNLLRAGINCRWSYHKISSDA
                     EGRSVNDRITQLMGNDLNVLVYNFVDMLSHARTEINIIRDLTADEASWLTLTHSWFIH
                     SPLMELLRFVSSRSARVVFTTDHGSVRVQRPLKVVGDRQTSMNLRYKTGRNLDYNPRE
                     VFEITAPAKAGLPMVNITSRYIFACANDFLVYPNNYNSVVSYYRDSFQHGGISLQEML
                     LPLVSLEPV"
     gene            4562..5002
                     /gene="tsaE"
                     /locus_tag="PKK03_04665"
     CDS             4562..5002
                     /gene="tsaE"
                     /locus_tag="PKK03_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNX83720.1"
                     /translation="MKIVIADKSRLHDAARHFLKETAGKRLFAFYGQMGSGKTTFIRA
                     LSHEMGVADTVTSPTFTLVNEYRRPGSTPVYHFDFYRIKKITEVLDFGIEEYFDSGSP
                     CFMEWPEMIEPLLPDETMRISITVAPDGSRIIESMETGTHSSPL"
     gene            5166..6362
                     /locus_tag="PKK03_04670"
     CDS             5166..6362
                     /locus_tag="PKK03_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNX83721.1"
                     /translation="MNEPARSAFLPREEQIEKAVGRKQMSIGLPRDVHDDEKRISLTP
                     EATKILTDAGNEIIMERGAGQAAGFTDREYSDNGAIVADTAAKVYECDIIIKTAPFKQ
                     KEASQLRVGQTVISFMNVATMGEDVLSTLMRKRVTALASERIKDSDGMLPVVESMSEI
                     SGVTSVLLASEYLSSTSGGKGVMLGGVTGVTPTEVIIIGANTAGEYAARAALGLGAIV
                     RVFDTSVHRLRNFQNLLGQRIYTSTFHPQVLQKALKSADVLIGALSTDILMPWFYVTE
                     EMVQGMKRGSVIIDLSVDTGGCVETAEPRAIKDPIYEKHGVIHYSAWNLPSRVPKTAS
                     IALSNVFRPLMQEIADAGGITHMLKFNPGLRSGVYIFNGILTNEVLGQKFGILSKDIN
                     LLLAAF"
     gene            complement(6447..7358)
                     /locus_tag="PKK03_04675"
     CDS             complement(6447..7358)
                     /locus_tag="PKK03_04675"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNX83722.1"
                     /translation="MKSLLIPPYLRDGDRIEIITPASPIEEGVVHNAVHMLEESGFRV
                     TLGSHVFARSGPFAGSEADRLHDIQRATDDPDVKAVLCSRGGYGMSRIVDRIDFSALK
                     KQPKWYAGYSDITSLHMWLSRVCGIASLHAEMPLNYSNPEYLPVCYETLVKALRGEAE
                     PVRWLPSHEASVEVSGPVTGGNISLLYSLHGTPAQPDTDGAILFIEEIGEHFYHLDRM
                     LVAMRMAGLLRNLSALVVGGMEKITEGEHVFNQTVEEVVMNVVAGYDYPVLFNFPAGH
                     IPDNRAIYLGRNVKIRQSGSEAVMSWL"
     gene            complement(7398..8564)
                     /locus_tag="PKK03_04680"
     CDS             complement(7398..8564)
                     /locus_tag="PKK03_04680"
                     /inference="COORDINATES: protein motif:HMM:NF040309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6051 family protein"
                     /protein_id="HNX83723.1"
                     /translation="MGYTASERQISSHFREGIAEGVIPGTGVTVAARRFSSAVDSSLP
                     DGQDVPGLFRSLFRSHEVEESNREFSYYILSPGGDRGKPCTGRGSIILLHGLNERSWS
                     KYIQWGARLADGTGRPVVLFPLAYHMNRSPRSWIDRHIMMPLVSARQALLPDARLSTF
                     VNVALSTRMTVSPQRFMLSGYQTAGDLTTLIRSLREGSIPGIAAGGPTDIFAYSIGAL
                     MTQVMMLSDPSPLPEDSRIMLFCGGSAFSMMNGTSKLIMDSRAFEMLLSFYVGWPDSA
                     MNGGRERLTRLMNNTPEGEAFYAMTSLQRLKDLRGRPFSRYDGRLKAVTFESDTVIPA
                     KAVMETLSGADTEVWNPGYPCTHESPFPVLAGDNANEADRTFDRLFDTAARFLS"
     gene            8757..9608
                     /locus_tag="PKK03_04685"
     CDS             8757..9608
                     /locus_tag="PKK03_04685"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNX83724.1"
                     /translation="MNIDLSKKLPVYLTEKGNIVTTILFTAGFALLFLNLYTPFGVGT
                     WFEVSKPTLLLYASGVILAGVLVVAISRILMYHHVKRGKSLLIWHYLIWVAAEIIAMA
                     LFFTLFEIVILHDQRPFPELLVRSVQNTSLTLLLPYTFLWLWFAWRESKKTLSGIMEQ
                     PETASQRPMIHLRDEKGVLRLSLKHTDLLYMQASDNYVTVFYQAKEKLTRYMLRNTLR
                     TLEEELKEEAIIRCHRSWLVNIEKVKLIRREKEGLLLELDSTPPSSVPVSRTYMHDVL
                     AAFGHVE"
     gene            9677..11710
                     /gene="metG"
                     /locus_tag="PKK03_04690"
     CDS             9677..11710
                     /gene="metG"
                     /locus_tag="PKK03_04690"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNX83725.1"
                     /translation="MKKFKRHLVTSALPYANGPIHIGHLAGVYVPSDIYTRYLRMRGE
                     DVISICGSDEHGVPITLKARKEGITPQEVVDRYHELNKKAFADFGIAFDIYSRTSNRI
                     HYETASQFFRKLYDQGEFVEKTASQYYDEEAGCFLADRYITGTCPYCGNENAYGDQCE
                     KCGTSLSPTDLINPKSTISGSAPVMRDTLHWYLPLDKYEPWIRKWILEEHTEWKTNVY
                     GQCKSWLDQGLQPRAVSRDLDWGVPVPVEGAEGKVLYVWFDAPIGYISATRELTPDWE
                     KYWKDPETRMVHFIGKDNIVFHCIIFPAMLKAEGSYILPDNVPANEFLNLENDKISTS
                     RNWAVWLHEYLQDFPGKQDSLRYALCASAPETKDNDFTWKEFQARNNNELVAILGNFV
                     NRTLVLTANYYKGTVPVPGVMNDYDKETVKSIMTCRENTESNLEAFRFRQALAEAMNL
                     ARLGNKYLADTEPWKVVKTDPERVKTIMYVSLQITANLAILLEPFLPFSMDRLRGWLG
                     IAGLHWDAAGRSDLLSPGHTVSSGELLFSKIDDADIEKQIQKLLDTKKANQNATAPVL
                     PQKEDVSFDEFTRMDIRTATILEAEKVAKTTKLIKLKVDTGIDVRTIVSGIAEHFTAD
                     ELPGKNISVIVNLAPRTIKGIESKGMVLLAEDENGKLVFVTPEKKIKNGSPVK"
     gene            complement(11796..12875)
                     /locus_tag="PKK03_04695"
     CDS             complement(11796..12875)
                     /locus_tag="PKK03_04695"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNX83726.1"
                     /translation="MARDISELIKELLLKHDCVIIPGFGAFIGNYFPARADRKDGLFE
                     PPSRKITFNRHLTGNDGLLIGHVSAVTGTGYGEARDIVNDWSGELRSKIMTGNPVMID
                     RLGTFSLNYDRAIVFEPDLTVNYLLSSYGLSAFHRKPVSGLDVRKMAIERRHTPAVSQ
                     PSMRSLLTRAAVIIPVLVALALVPFNDRIFRSNIEESNLNPLARAELEFNREQIDADR
                     SAAAPEINITRDTGNPDNTVNEMKASLGLTVQDSGPVSGPAETAVKTAPDTAVNRAPA
                     RRTTAVVVEEYRFMVIIGSFKGEDNALVMVEKLRRQGFDPEVAGGPDGFLRVSAQAFP
                     TLDEAKVVLEKLRREYPGAWVHKSR"
     gene            12980..14107
                     /locus_tag="PKK03_04700"
     CDS             12980..14107
                     /locus_tag="PKK03_04700"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNX83727.1"
                     /translation="MKKIEMVDLRSQYERLKADIDNAITGVISSTAFIKGPDVRLFEE
                     ELQRYLGNRHVISCANGTDALQIAMMALGLKPGDEVITTNFTFIATVEVIELLGLKTV
                     LVEPDYGSFNISVEAIRRAITPRTRAIVPVHLFGQCADMEPILDLARQHDIHVIEDVA
                     QATGAEYRFSDGTVKKAGTMGTIGCTSFFPSKNLGCYGDGGALFTDNDELAALIRSIT
                     NHGMKVRYYHDTIGVNSRLDTIQAAILRVKLRHLDAFNEARARAAAFYDTQLSGLSGI
                     TVPERSAFSSHIFHQYTLRIAGGERNSLRECLENEGIPAMIYYPVPLHMQKAYLHLGY
                     RPDDFPVTTNLSGEVLSLPMHTELDDEQLAYITGCIRSKFN"
     gene            14112..14690
                     /locus_tag="PKK03_04705"
     CDS             14112..14690
                     /locus_tag="PKK03_04705"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNX83728.1"
                     /translation="MEKEYFAHPTAVIDEGCTIGNGTKIWHFSHIMKNSVIGTNCNIG
                     QNVVVSPGVVLGNNVKVQNNVSIYTGVICEDDVFLGPSMVFTNIINPRSAVIRRDQYV
                     TTMVRHGASIGANATIVCGNEIGQYALIGAGTVITKSVKPYALVVGNPGRQTGWVSEY
                     GHKLSFDEGGLAVCPESGQQYRLSGGEVTRII"
     gene            14719..15669
                     /locus_tag="PKK03_04710"
     CDS             14719..15669
                     /locus_tag="PKK03_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX83729.1"
                     /translation="MKTAPRNFGLIGIAGYIAERHLRAIKDTGNRLLASLDKFDCVGK
                     IDSYFPESDFFVEFERFDRHVDKLKRSGVQLDYVSICSPNYLHDSHIRFALRHQADAI
                     CEKPIVLNPWNIDALQEIEKETGRHIFNILQLRYHPVIMNLRESIKTMPQNKKHDIDL
                     TYITSRGNWYHISWKGDVNKSGGIATNIGVHFFDMLGWIFGPVVSNRVHLLGRDKASG
                     YLELENARVRWFLSIDWNDLPADVKEKGKRTYRNITVDGEELEFSEGFTDLHTVTYRE
                     ILGGNGYGLEDARQSIETVYQIRNSALSVLTGEYHPLIKK"
     gene            15672..16961
                     /locus_tag="PKK03_04715"
     CDS             15672..16961
                     /locus_tag="PKK03_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNX83730.1"
                     /translation="MYDKLVNRETKLSLIGLGYVGLPIALEFAKKISVVGFDIRPDRV
                     ELMKKGIDPSNELEHEAFEGTDIKFTADPQDLREASFHIIAVPTPVTRHNLPDLEPVL
                     KASETVGRILKKGDYVVYESTVYPGCTEEECIPVLERYSGLKCPADFKVGFSPERINP
                     GDKNHTLTKIVKVTSGCDAESGENIAQTYEMIIKAGVHRASSIKVAEAAKIIENTQRD
                     INIAFMNELSIIFNRMGINTYEVLEAAGTKWNFLKFYPGLVGGHCIGVDPYYLSYKAK
                     AMGYHPQMIDSGRFTNDSMGAYAGKETVKKLIAAHKSPDRSRVLVMGMTFKEDVTDIR
                     NSKVIDVVNELRDFGVEVDVIDPGASHAEVKHEYGIDLKKGPEGKYDAIIVAVSHMEY
                     RDLDEKFFLDLLNEKGILVDLKGLYRNNMKWLTYWSL"
     gene            16961..17992
                     /gene="galE"
                     /locus_tag="PKK03_04720"
     CDS             16961..17992
                     /gene="galE"
                     /locus_tag="PKK03_04720"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005829134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNX83731.1"
                     /translation="MNGKVIVTGGTGYIGSHTAVELITAGFEVVIIDNLYNSDISVLD
                     GIRSITGVTPKFYDYDLCDSQKLEEVFGAEGKVDAIIHFAAYKAVGESVRKPLEYYRN
                     NLTSLMNLLEAMERHNTTALVFSSSCTVYGQPDTLPVTESSPVKDAASPYGYTKQAGE
                     VIIRDTMASRPGQKAISLRYFNPIGAHPTAFIGELPRGVPENLVPYITQTAIGLREEL
                     KVFGTDYDTPDGSCIRDYLHVVDLAKAHVVALKRLLGGENKANYEVFNLGMGKGVSVL
                     EAIAAFGKATGVRLNYSVTGRRAGDIEKIWADPSLANRELGWKAGISIEEAMETAWRW
                     ENKLKNKKS"
     gene            17989..19044
                     /gene="rfbB"
                     /locus_tag="PKK03_04725"
     CDS             17989..19044
                     /gene="rfbB"
                     /locus_tag="PKK03_04725"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNX83732.1"
                     /translation="MNRTILITGGAGFIGSHVVRLFVNKYPEYKIVNADNLTYAGNLG
                     NLTDIGNRPNYLFRKVDIVDKKAVNALFDEFRFDGVIHLAAESHVDRSITSPDEFIHT
                     NILGTVNLLAAFRDVVPASGADKRFYHISTDEVYGSLEHGGFFTEQTPYDPRSPYSAS
                     KASSDHMVRAFGNTYKLPFVISNCSNNYGPNQFPEKLIPLTINNIKTMKPIPVYGKGE
                     NIRDWLYVEDHASAIDVIFHRGKPGETYNVGGDNEWKNIDLVRLLCRIMDRKLGRNDG
                     DSEKLITFVTDRAGHDLRYAIDSSKLQRELGWKPSVTFEQGLGMTVDWYLSNEKWLTA
                     IISGDYAKYYEEMYGSR"
     gene            complement(19140..21137)
                     /locus_tag="PKK03_04730"
     CDS             complement(19140..21137)
                     /locus_tag="PKK03_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008640811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HNX83733.1"
                     /translation="MADGYRSLSIGEIRGLEAAGCRSNSWESVFVKDPFNAERIQNVT
                     FSGTVRLGLFNGVARERCGLPLMHGIKNAHIHNCTIADNVTITDITGFIANYDIGEGV
                     VIRNCGHISTEGHTSFGNGTRVAVLNETGGREVPIWDRLTSHMAYIITLYRHRPSAIA
                     EIEKLIDDYAESVKSDRGEIGSGSYIAGCGTIKNVRIGNGAVIEDVSRLAEGSVNSSF
                     RDPVYIGNDVIMDHFIICSGSKVNDAAVIDKCFIGQGCTLSKQYSAENSLFFSNCGGY
                     HGEACSIFAGPYTVTHHKSTLLIAGIFSFMNAGSGSNQSNHMYKLGPIHQGIMERGSK
                     TTSDSYVLWPSRIGPFTLVVGRHYHNVDTTSFPFSYLTEVSYESQLIPGINLRSVGTI
                     RDAQKWPKRDMRKDTKKLDQINYNLLSPFTVGKMMKGRDLLKVLIDANHGQEIITYER
                     MRMKISAAGRGISLYQMGIDKFIGNSVIKRLEGTVFKSDAEIRDRMKPDTNGGTGDWI
                     DMSGLITPLSSIEELLAAIERKEMKSADAINRSFENIHKHYYNLEWTWVYDHLPRETG
                     KPNEELTADDIIAIVERWKKSVVDLDRMLYEDARKEFTLSSMTGFGIDGDDEVKKRDF
                     EQVRGDFEKNGVVLAILDHIKAKTELGDELIGRLKVIRSEV"
     gene            complement(21153..22991)
                     /gene="glmS"
                     /locus_tag="PKK03_04735"
     CDS             complement(21153..22991)
                     /gene="glmS"
                     /locus_tag="PKK03_04735"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNX83734.1"
                     /translation="MCGIVGYIGSRPARDIVINGLKRLEYRGYDSAGIAISNGERTEI
                     FKCAGRVRELEEMVRNKDFDGSIAMGHTRWATHGEPNEINAHPQVSCRGHFIVVHNGI
                     IENYARIKKHLESRDVSFTSQTDTEVLANLMEHLYLEGDLSAEQAIIMALNKVEGTYG
                     IAVYCRDEPGKLFAARLGSPLVIGVGDGENFIASDATPIVEHTQRVIYLNDDDLAVIR
                     KDEMVLKAIRNKRVEPEVKEVDLKLGEIDKEGFPHFMLKEIFEQPRAIHDTFRGRVLP
                     NQSGLMLGGLHNVFDTMATADRVLIVACGTSWHAGLVGEYIIEEYCRIPVEVEYASEF
                     RYRDPVIKKGDIVLAISQSGETADTLAAIRLAKERGALVLGICNVVGSSISRETNAGV
                     FTHAGPEIGVASTKAFTTQVVTLAMVAFEIGYRKGLIDEVRYRGLIQELTSLPGKVDI
                     ALKMNDKALALAKIYKDSTNALYLGRGLLYPVAMEGALKLKEISYIHAEGYPAAEMKH
                     GPIALIDEHMPVVVVATKENSYDKIVSNIQEVKARKGQIIAVVTEGDQVISHMADHVM
                     EVPETIGVFAPVLAVVPLQLLSYHIAVLRGCDVDQPRNLAKSVTVE"
     gene            complement(23015..24325)
                     /locus_tag="PKK03_04740"
     CDS             complement(23015..24325)
                     /locus_tag="PKK03_04740"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HNX83735.1"
                     /translation="MTNSFWNLQNNKPGNTRKGAPSIILTLSALLTAALLSCSQDPVK
                     IGIDLLPDSDFIDIGSTDTVSIKAYTMYDELSVSSDYTTMIIGSLYNEYFGTTYCDFV
                     TQLRLTDKWPAKPYVIDSVLLTMFPSDVSGDDSTSVHRIRLCETGTKLTDTAEYYAGQ
                     DPDTIKFLGEYVLPTMKAGTPLKVKLDNSVGEYLLRDTTKLSRGAPFYEDYFKGLYCM
                     IKSSPNPVLVVMDASERASVDPLGITIFYKSDTLRHSYSFVATPRAVNYNRFTHDRST
                     ADPAKQILHVNDLVADTAVFVQTYNGVYVKLDMPTLEPFRDVDNLAVNKARIIAPVIL
                     DGEILLDKTMPQRVYVRYRDADGKVQVIPDYLHDISFMDGTYYSDNDYYIFNITTFVQ
                     KYLDGEIEEPSVELFLPLTSTRNAVFRANANEPAFKLEFAYTIY"
     gene            complement(24294..25115)
                     /locus_tag="PKK03_04745"
     CDS             complement(24294..25115)
                     /locus_tag="PKK03_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNX83736.1"
                     /translation="MESRRVLFVSQEIMPYLGETKLSKISRDLPQGIQERGKEIRTFM
                     PRYGSINERRNQLHEVIRLSGMNLIIDDTDHPLIIKVASIQSARMQVYFIDNDDYFQR
                     KHTVADAAGNEYEDNDERAIFFARGVIETVRKLRWSPDIVHVHGWLASLVPLYLRKLY
                     SDDPVFSKVKSVYSIYNNGFKKPLKSSLSEKLLADGIDTGTLKSIKGSADFLNLTALA
                     AENSDGIIFGCEKIDEQVKKRITAMGKPLLEYQGEENYIDAYNDFYDKLLLESAK"
     gene            25291..26148
                     /gene="panC"
                     /locus_tag="PKK03_04750"
     CDS             25291..26148
                     /gene="panC"
                     /locus_tag="PKK03_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNX83737.1"
                     /translation="MKVLTTAKEVREHYRISVLRNPGFVPTMGALHEGHLSLVARAAE
                     RCRMVAVSIFVNPTQFNDPQDLLKYPRTLDADLEMLSGLLRENDFVFVPSVEEIYPEP
                     DTRQFDFGELEKVMEGLHRPGHFNGVAQVVSRLFDIIRPSYAFFGQKDFQQLTIIREL
                     ARREYPKVEIIACPIVREHDGLAMSSRNRRLLTQHREKAGEIYRTLIAAAAMISDYEI
                     NEIREFVADRINMIPDFRLEYFEIVEEGTLKPVHSVIEMSPEKKYFGCIALWAGEIRL
                     IDNIEIGLR"
     gene            26199..>26306
                     /locus_tag="PKK03_04755"
     CDS             26199..>26306
                     /locus_tag="PKK03_04755"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNX83738.1"
                     /translation="MTIEVLKSKIHNVTVTGADLNYVGSVTIDEDLMDAA"
BASE COUNT         6516 a         7024 c         6821 g         5945 t
ORIGIN      
        1 agcaggcttg ccgccgagag cggcaccttg cttctggggc agatacccat cgtggagagc
       61 atctgtgacg gcggtgactc tggcaggccg gtggcgctgg gagacaacgc aacaggaact
      121 gctttcatga acctcgctga gacagttgta aggcaggtgg agatacgcaa cgcggagatt
      181 gccccgacga agagggttga tgtaaagtga gcctgctgcc gccgccggca gtcttacccc
      241 ttttttcccg cctctttttc ttaactttgg atactttcgg agtacctctt cagttataag
      301 agtataaacc atatttgttg agattacggc atagggaata cactgcagcg gtgatggtca
      361 ttatcgccct gattcttgcc gggtgttctg ctgagaagaa caccggtgcg agccgttttt
      421 acaattcact gatctcaaag tacaatatct ggttcaacgg caatgaagcc tacaaggccg
      481 gtgtgaagaa ggtgagagag tcacaccgcg atgactacag tgacctcctt cccgttttcg
      541 aatactccga tgcggagtcg gcccgcatgg cctcatccga catggaacgt gccgttcaga
      601 aagcttccaa agtgatagcg ctgcactcca taaccgcaaa gcctgaagaa aagaaccgga
      661 ggaacgcatc cgcgaagagc gaggagtttt acaacaggag ggaattcaac gaatgggttg
      721 atgactctta tctccttctt ggcaaggcgc agttctacca gaagaattat atggcagccc
      781 gctcctcact ctcatttgcc gtaagcattg ccactgaccg cgacctcgtc accgaagccg
      841 ggatatggat ggcacgcatt cagactgagg aaggaaatta tacagaggca ttgcgcgtgc
      901 tgcaggagac agccagtcct gagaacctgt cacgcccgct gaaggagatg tattactcca
      961 cacgtgcaga tatcattctc aggcagaaga agtatcgcga ggcacttgag cctctcacaa
     1021 ccgccactga gtacgccggg aacaacagga caaaggtgcg gctgacctat ctccttgccc
     1081 aggtctgcag ggccgccggt gataatgacc tctcaacacg ctacttcagc caggtgatga
     1141 aaatgaatcc tccctatgac ctggaattca acgcaggcat caacctggca ggagtgaccg
     1201 atatctcgca cggcgacgct gatgaccttt ccaaatcact gcgaaagatg cttcgcgact
     1261 ccagaaacaa ggattatctt gaccagatct acttcgccct gggtgagctc gagcagcgcc
     1321 ggggaaacac tgacgaagcc ctgaagctct ggtcacaggc agcagccagc agcactgtca
     1381 ataccaggca gaaggcacgt tcataccttg ccattgccgg acacttttac gagaagcctg
     1441 attaccttaa gtcatggcaa tactatgaca gcgcctcata cctcatagac aaccgtttcc
     1501 caaactatga gatgatcagc cgcaggacct ctgacctggg cgaatatgca ggctttcaca
     1561 ccgtggtgat aaccgaagac agcctccgca gggtggccgc catgagcact gccgagcgcg
     1621 atgcactgat agcgggcgtg ataagaaacg tagaggcgga gatgaccagc cagcgaaccg
     1681 gcgacggcag cgacaggtac aacatggggc agtattatga gaacgagcag cgataccgcg
     1741 gcgccatctc ggccgaggga ggatggtatt tctacaacca ggcggctctc accttcggac
     1801 gtactgaatt caggcgccgg tggggagaca gaagactcga ggataactgg aggaggacaa
     1861 acaagaccag atcggcattc gccccaggaa cacagcagga agctgagaac cagaaggaaa
     1921 aggctgacag cgccggagtg gcgccggaac ggacaaaaga atattatctc cgcaacctgc
     1981 ctctaacaga ctcgctcctt aaaatatcag tgaaacggag tgcggaggcg ctccttggag
     2041 agggcaagat acttgcatcg aggctcaatg acactctctc tgccgccacc tcactggaag
     2101 aagccgccag aacaggaact gatgacaaca ccagggcaga agccctttac gaactatacc
     2161 gtctgctgag caaaaccaac ccgtccaggg ctgacaggcg cagggccgag ctgctggcat
     2221 catatcccga ttcggaatat gccctcatcc tgtccgaccc tgattatgcc aacaggcaca
     2281 aggagatggc agcaagggct tcgcgaagct atgacgttgc ttacctggca tttaccgaag
     2341 aaagatttgc agatgcaatc agcctctgca ccgaagccga aagactctat cccgaagatg
     2401 aactgttgcc aaagttcatg cttctcgatg ccatggccac tggcgccctg gagggcgaaa
     2461 tggcttacaa ggcaaaactt gactcgctgg tggcgaaatt tccctcaaca cctgaagggc
     2521 aaagggccgc cgagatcacc gacttcctcc gcaaggagaa accggagatc aggattgcag
     2581 aggatacccg aatagcggaa gaaatataca ttgcggacac actgcagccg cattatgtta
     2641 taatcattgc ttccaatccg cgtgcaaaca tgaaccagat ggtgtttgac atcatcaact
     2701 acaacctcga cgtgtaccct gacaggagct atcgcaccga aggagctgca atagacgccg
     2761 ggtaccttct tatcaccgtc ggtccgtttg aaaaaacagc cgatgcagtg acatggtaca
     2821 ggtcattcaa ccccgaacag gctgtcaggg aggcagcaac cgcaggcctg gcagtatatc
     2881 ttatcagccg cgacaacctg cagaaattcc gggaagacaa gaataccgac agatatgcta
     2941 tctttcactc gaaagcgtat cccaaccttc ggtaacctga aaggctgcaa atgagacaaa
     3001 tacgaattct ctgggtcgat gacgagatag gggttctcag gtcacatatc ctttacctcg
     3061 aagaaaaggg ctttgagact gacacatgcc attcaggcaa tgatgcaatc ataatggtca
     3121 gggagaaagc ctatgacatc atcttccttg atgagcatat gcctggcctc agcggtattg
     3181 agaccctgcg ccggataaaa gccataaggc cggccatccc cgttgtcatg atcaccaaga
     3241 gcgaggagga ggatatcatg gacgccgcca tcggttcaca gatagccgat tacctcatca
     3301 agcccgtcaa accgcagcag atactgctga cccttaagcg catactgaac atcgaggaga
     3361 ttgtggcaag acaaaccacc accgtcttcc gggaagagtt cagcagcatt gctgacatga
     3421 tctcatcagc ctccactttt gaacagtggt gcgaactcta ccgccggctc accttctggc
     3481 agggagaact gatcaggtca gcagacaccg gcatgcgtga ggtgcttcgc atgcaggagt
     3541 cagacgccaa cagggctttc gcaaaatata tatccggcaa ttacatgaag tgggtttccg
     3601 ccgaggcggc tgacagaccc cttatgtcgc cggctgtgat gtcgaagaga atttttccca
     3661 tgatcagcaa ggggactccg ctctttttca tcatcattga caacatgcgc ctcgaccagt
     3721 ggcagaccat agcaggcgac ctgcggcagg tgatgcgcac cgttgatgag gagctctacc
     3781 tgagtatcct ccccaccacc acccagtact cgcgcaacgc actctttgcc ggtctcatgc
     3841 cggctgccat cgaggaattg atgcctgaat actggattga cgagcatgag gatgaaggca
     3901 agaacctgaa cgaggagaaa ctgctggcaa agaacctgct cagggccggt atcaattgcc
     3961 ggtggagcta tcacaagatc agttccgacg ctgagggcag aagcgtcaac gaccgtatca
     4021 cgcagctcat gggcaatgac cttaacgtgc tggtatacaa ctttgttgac atgctgagtc
     4081 atgcccgcac cgagatcaac atcatccgtg acctgactgc tgacgaggcc tcctggctca
     4141 ccctgaccca ctcgtggttc atccactctc cgctgatgga gctgctgagg tttgtctcct
     4201 cccgcagtgc acgggtggta ttcaccactg accacggatc ggtcagggtg cagcggccgc
     4261 ttaaagtggt gggcgacagg cagacatcga tgaacctccg atacaagaca ggaaggaacc
     4321 tcgattacaa tccgcgcgag gtgtttgaga tcaccgcacc tgcaaaggca ggactgccca
     4381 tggtgaacat aacctccagg tatatttttg catgcgcgaa tgacttcctg gtctacccga
     4441 acaactacaa cagcgttgtc tcctactacc gcgactcctt ccagcacggt ggcatatcgc
     4501 ttcaggagat gctcctgccg cttgtctccc tcgaaccggt ctgatttgct atttttgcag
     4561 catgaaaatc gttattgctg acaagagccg actgcatgat gctgcaaggc atttcctgaa
     4621 ggagacagca gggaaaaggc tctttgcctt ttacggtcag atgggctccg gaaagacaac
     4681 ctttatcagg gcgctgagcc atgagatggg cgtggccgac accgtcacca gtcccacctt
     4741 taccctggtc aatgagtacc gcagaccggg cagcacacct gtttatcact tcgactttta
     4801 caggataaag aagattaccg aggtactcga cttcggcatc gaggagtatt ttgactccgg
     4861 ctctccatgt ttcatggagt ggcccgaaat gattgaaccg ctgctgccgg atgagactat
     4921 gagaatctca atcaccgtcg cccctgacgg aagcaggatc atcgaatcca tggaaaccgg
     4981 gacgcattcg tctcccctct gaaccccggg gtatcttaag taacataatc cgcaacgcct
     5041 tctgttccct gcactgctgg ctcttctctg atttatgccg ttcttcctgt catctgtgca
     5101 attaaaatct taattttgat ctggtgagtt ttttcctcac tcctaacctt ttaaaacgaa
     5161 gccagatgaa tgaaccggcc agaagcgctt tcctgccgag ggaagagcag attgaaaaag
     5221 ccgtgggcag gaaacagatg tcgattggtc ttccccgtga tgttcatgat gatgaaaaac
     5281 gaatatcact tacccctgaa gcgaccaaga tcctcaccga tgcaggcaat gagatcatca
     5341 tggagagagg tgccggacag gccgccggct tcaccgacag ggaatacagc gacaacggtg
     5401 caattgtggc tgacactgca gccaaggtat atgagtgtga cattattatc aagacagctc
     5461 ccttcaagca gaaggaagcc tcacagctca gggtcgggca gacggttatc tcattcatga
     5521 atgtcgccac catgggcgaa gatgttcttt cgacactcat gcgcaagagg gtgacggccc
     5581 tggcaagcga aaggataaag gattcagatg gaatgcttcc ggtggttgag tcgatgagtg
     5641 agatcagcgg tgttacatcg gtgcttctgg cttccgaata tcttagctcc acttccgggg
     5701 gcaagggagt gatgctcgga ggagtgaccg gggtgactcc cactgaggtg atcataatcg
     5761 gcgccaacac ggccggcgag tatgcagccc gggcagcgct ggggctgggg gccatcgtga
     5821 gagtgtttga cacgtcggtg catcgcctcc gtaactttca gaacctgctg gggcaaagaa
     5881 tatacacttc gacattccac ccacaggtgc tgcagaaggc gcttaagtcg gctgacgttc
     5941 tcatcggagc cctctccaca gatattctca tgccctggtt ttacgtcacc gaggagatgg
     6001 tgcaggggat gaagcgcggt tcggttataa tcgatctcag tgttgacacc ggcggctgtg
     6061 ttgagacggc tgagccgagg gcaataaagg acccgattta tgagaagcat ggagtgatac
     6121 actactctgc ctggaacctc ccttcgcggg tgcccaagac agcatcaata gctctgagta
     6181 acgtcttcag gccgctgatg caggagatag ctgacgccgg aggcataacc catatgctca
     6241 aattcaatcc cggactccgg agcggtgtct atatcttcaa tggcatcctc accaatgagg
     6301 tgctgggcca gaagttcggt atcctgtcaa aggatatcaa cctgctgctg gcagcgttct
     6361 gaccagtcgg caggaagagg tgtgtgtgcg atttgcgatt tgcgattttc cccggcgggg
     6421 agcaagcatt gtagccctgc cgcgattcaa agccagctca taacagcctc actgcctgac
     6481 tgtctgatct ttacattcct gccaaggtaa attgccctgt tatccggtat atgtcctgcc
     6541 gggaagttga agaggacggg ataatcatac ccggctacca cattcatcac cacctcttca
     6601 acggtctggt taaacacatg ctcaccctct gttatctttt ccatacctcc caccaccagg
     6661 gccgacaggt tcctcagcag gcctgccata cgcattgcca ccagcatccg gtcaagatgg
     6721 tagaagtgtt ctcctatttc ctcaatgaac aaaattgccc cgtctgtgtc aggctgtgca
     6781 ggcgttccgt ggaggctgta tagcagcgat atgttgccgc cggtgacagg gccggagacc
     6841 tcaaccgaag cctcatgcga aggcagccag cgcaccggtt cagcttcacc gcgcagcgct
     6901 ttgaccaggg tctcatagca taccggaaga tattcaggat ttgagtagtt aagtggcatc
     6961 tcggcatgca gtgaggcaat gccgcatacc ctgctgagcc acatatgcag tgaggtgata
     7021 tcgctgtagc ccgcgtacca cttaggttgt ttcttcagag ctgagaagtc tatccggtca
     7081 acgattcttg acatgccgta accgcctcgt gaacagagta cagcctttac atccgggtca
     7141 tccgtagccc gctggatatc atgcagccgg tctgcttcac taccggcaaa tggaccgctg
     7201 cgggcaaaaa catggctgcc aagcgtgaca cggaagcctg attcctcaag catgtgcacg
     7261 gcattgtgta ccactccctc ttcaataggg ctggcagggg tgatgatttc aatacggtcg
     7321 ccatccctga ggtatggcgg aatcaaaagt gatttcatag actctcctga tacggtgaag
     7381 tatcaaaaat agtcatttca tgaaagaaat cgcgcagcag tgtcaaagag cctgtcaaag
     7441 gtccggtcag cctcatttgc attatccccg gcaagaaccg ggaaaggtga ctcatgagta
     7501 cacgggtatc ccggattcca gacctcggta tctgctccgc tgagtgtctc cattacagcc
     7561 tttgccggaa tgacggtatc tgattcaaat gtcactgcct taagcctgcc gtcatatcgg
     7621 ctgaatggtc ttccccgcag gtctttcagt cgctgcagcg aggtcatggc ataaaatgct
     7681 tccccctcgg gggtattgtt cattaacctg gtaagtcgct ccctgccgcc attcatcgca
     7741 ctatcaggcc aaccgacata gaatgagagc agcatctcga aagcgcggct gtccattatc
     7801 agcttcgagg ttccgttcat catgctgaag gcgctgccgc cgcagaagag catgatgcgg
     7861 ctgtcctcag gcagcggtga cggatcggag agcatcatga cctgtgtcat cagcgccccg
     7921 atggagtagg caaaaatatc tgtcggcccg ccggcagcta ttccggggat gctgccttcc
     7981 ctgagtgaac ggataagcgt tgtaagatca ccggcggttt gataaccgga aagcatgaac
     8041 cgctgcgggg agactgtcat cctcgtgctc agcgccacat tgacgaaggt tgagagcctg
     8101 gcgtcaggca gcagcgcctg gcgcgctgac accaggggca tcatgatatg cctgtcaatc
     8161 cagcttcgtg gtgagcggtt catgtgatac gccagcggga aaagaaccac cgggcgtccg
     8221 gttccatccg ccagtcgcgc accccactga atgtactttg accagctccg ttcattaagg
     8281 ccgtgaagaa gtataattga accgcgcccg gtgcaaggtt tgcctctgtc cccgccgggg
     8341 ctcaggatat aataactgaa ctccctgttg ctctcttcca cctcgtgtga gcggaaaaga
     8401 gaacgaaaca gtcccggcac atcctgcccg tcaggaaggg agctgtcaac ggcagagcta
     8461 aacctccggg cagcaacagt caccccggtg cccgggatca ccccttccgc gatgccctcc
     8521 ctgaagtgtg aggagatttg ccgttcactg gctgtataac ccatatctga attttagggc
     8581 aaacaaaaca caggatgagc ggcactccaa aaaataggtg gctccccccc gccggtgggc
     8641 cgaaatcctc cgggcagggg cgaaatacaa cattcagggg tgaaaattcc tgccatactt
     8701 caggccggaa acggcagatc cgcaaaagtg ttacttttgc aattgcaact gatcccatga
     8761 atattgacct gagcaaaaag ctgcccgtgt accttacgga aaaggggaat attgttacca
     8821 cgatactctt cacagccggc tttgcactgc tctttctcaa cctgtacacc ccctttggtg
     8881 taggcacctg gtttgaagtt tcgaagccca ccctgctgct ctatgccagc ggtgtcatcc
     8941 tggccggagt gctggtggtg gcaataagcc gcattctgat gtaccaccac gtcaaacgcg
     9001 gcaagagcct cctcatctgg cattatctta tctgggtagc cgctgagatc attgcaatgg
     9061 ctctcttctt cacccttttc gagatagtca tcctgcatga tcagcgcccc tttcccgagc
     9121 tcctcgtaag gtcagttcag aacacctcgc tcacgctgct tcttccctat acattcctgt
     9181 ggctctggtt cgcatggcgc gaaagcaaga aaacactctc cgggattatg gagcagcccg
     9241 agacagcttc gcagcgcccg atgatacacc tccgtgacga aaagggagtg ctgaggcttt
     9301 cactgaagca taccgacctg ctttatatgc aggcaagtga caattacgtc acagtctttt
     9361 accaggccaa ggagaaactg accaggtaca tgctgcgaaa tactctcagg accctggagg
     9421 aggaacttaa ggaggaggcc ataatccgat gccaccgctc atggcttgtg aatattgaga
     9481 aggtcaagct gatacgcagg gagaaggagg ggcttctcct ggagctcgac agcaccccgc
     9541 cgtcatcagt accggtgtca cgcacgtaca tgcacgatgt gctcgctgcc ttcgggcatg
     9601 ttgaatagct tgcatcccga cgctttttga aaatcattat ctttgccggg ttaaaatcag
     9661 caatcatttt cctcaaatga agaagttcaa gcgccatctg gttacatcag cccttccgta
     9721 tgccaacgga ccaatacaca tagggcacct tgcaggggtt tacgtgccgt cggatatcta
     9781 tacccgttac ctccggatgc gcggcgagga cgtcatttcc atctgcggct ctgacgagca
     9841 tggagttcct atcaccctta aagcccgcaa ggagggcatc accccgcagg aggtggttga
     9901 ccgctaccat gagctcaaca agaaagcctt tgccgacttc ggcatcgcct ttgacatcta
     9961 ctcacgtaca tctaacagga ttcactacga aaccgcctcg cagtttttcc gcaagctgta
    10021 tgaccagggg gaattcgttg agaaaaccgc cagccagtat tatgacgagg aggccggttg
    10081 cttcctggct gacaggtata tcaccggcac ctgcccttac tgcggcaatg agaacgccta
    10141 tggcgaccag tgcgagaagt gcggaacatc tctcagcccc actgacctta ttaacccgaa
    10201 atcgaccatc agcggcagcg ccccggtgat gcgtgacaca ctccactggt accttccgct
    10261 tgacaagtac gagccctgga tcaggaagtg gatcctcgag gaacacaccg aatggaaaac
    10321 taacgtttac ggacaatgca agtcgtggct cgaccaggga cttcagccca gggccgtcag
    10381 ccgtgacctc gactggggcg tgcccgtacc cgtcgagggc gctgagggta aagtgctgta
    10441 tgtctggttc gatgccccca tcggatacat ctctgcaacc agggagctga cacctgactg
    10501 ggagaagtac tggaaagacc cggagacccg catggtacac ttcataggaa aggacaacat
    10561 cgtgttccac tgcatcatct tcccggccat gcttaaagca gaggggagct atatccttcc
    10621 cgacaatgtg ccggccaatg agtttctgaa tctcgagaat gacaagatct ccacttcgag
    10681 gaactgggca gtgtggctgc atgaatatct ccaggatttt cccggcaaac aggactcgct
    10741 tcgctatgcg ctctgtgcaa gtgcacccga gaccaaggac aacgacttca cctggaagga
    10801 attccaggcc cgcaacaata acgaactagt tgccatcctc ggcaacttcg tcaaccgcac
    10861 actggtactg acggcaaact actacaaggg cactgttccc gtcccgggcg tcatgaatga
    10921 ttatgacaaa gagacggtga agtcgatcat gacctgccgt gagaacacgg agagcaacct
    10981 tgaggccttc aggttccgcc aggcgcttgc cgaggcgatg aaccttgcgc gcctcgggaa
    11041 caagtacctt gctgacaccg aaccgtggaa ggtggtcaag acagatcccg aacgggtgaa
    11101 gactatcatg tatgtctccc tgcagatcac agccaacctg gcaattctgc tcgagccgtt
    11161 cctccccttc tcgatggaca ggctgagagg atggctcggc atcgcagggc tgcactggga
    11221 tgccgccggc cgttcagatc tcctttctcc cggccatacc gtatcatcag gcgagctgct
    11281 attcagcaag atcgatgatg cagatattga gaaacagata cagaagcttc ttgacaccaa
    11341 aaaggcaaat cagaacgcta cggctcctgt cctgcctcag aaagaggatg tctcctttga
    11401 cgagttcacc cggatggata tccgcacggc aacaatactt gaggccgaga aggtggccaa
    11461 aaccaccaag ctgataaagc tgaaagttga cacggggatt gatgtgcgta ccatcgtatc
    11521 gggcattgcc gagcatttca cagccgatga actgcccggg aaaaatatat cggtgattgt
    11581 caacctggcc ccgcgtacca tcaaaggcat agagtcaaaa ggaatggtac tgctggccga
    11641 ggacgagaac ggcaaactgg tgtttgtcac ccccgagaag aaaatcaaga acggaagtcc
    11701 cgtaaaataa ggatttgcga gttgcgatct gcgggttgcg accgcgaaca cggtagtgct
    11761 gactggtact gtctattgct gactgctact gccgactacc tcgacttatg gacccacgct
    11821 cccggatact cccggcgcag cttttcgagc acaaccttcg cctcgtcgag cgtggggaat
    11881 gcctgtgcac tgaccctgag gaacccgtcg ggtccgccag ccacctccgg gtcaaatccc
    11941 tgacggcgga gtttttcaac catgacaagg gcattatcct cccccttgaa actgccgatg
    12001 atcaccatga acctgtactc ctccaccaca accgcagttg ttctcctcgc aggagccctg
    12061 ttcaccgcgg tgtccggggc ggttttgacc gccgtctccg ccggcccgga aactggtcct
    12121 gaatcctgaa cagtcagccc gagagaggct ttcatctcat tgactgtgtt atccggattg
    12181 cccgtatccc gggtgatatt gatttcaggc gcagcagctg acctgtcggc atcaatctgt
    12241 tcacggttga actcaagttc agccctggcg agggggttga ggtttgattc ctcaatattg
    12301 cttctgaata tccggtcatt gaaaggcacc agggcaagag ccaccagcac cggaattatc
    12361 acagcggccc ttgtcagcag gcttctcatc gaaggctggc tgacggcagg ggtatggcgc
    12421 ctttcaatgg ccatcttcct gacatctaag ccactcaccg gtttccggtg aaaggcgctg
    12481 agcccgtatg aagagaggag gtaattaact gtaaggtcag gttcaaacac aatcgccctg
    12541 tcataattca gcgagaaggt tcccagccgg tcaatcatca ccgggttgcc ggtcatgatt
    12601 ttgcttctca gttccccgga ccagtcattc acaatatcgc gtgcctcacc gtatccggtc
    12661 cctgtcacgg cagagacatg gcctatcagc agtccgtcgt tcccggtgag gtgacggttg
    12721 aaggtgatct tccttgaagg gggttcaaag agaccatctt tccggtcagc gcgtgcggga
    12781 aaatagttcc cgatgaacgc accaaacccc ggtattatga cgcagtcatg ctttaaaaga
    12841 agttccttga taagttcgct gatatccctc gccatgtata gcaaataatg aacaaagata
    12901 aaaaagaaac ctgatccggc tccggaaaaa agggttaatt aaaatctatt atcttcgcta
    12961 ttaagatagc gttcagaaga tgaaaaagat agagatggtc gacctccggt cacagtatga
    13021 gaggcttaag gccgacattg ataatgcaat taccggggtg atcagttcca ctgccttcat
    13081 caagggtcct gacgtacgtc tcttcgagga ggagttgcag cgttaccttg gcaaccgtca
    13141 tgtcatctcc tgcgccaacg gcacagatgc tctccagata gctatgatgg cactggggct
    13201 caagcctggt gatgaggtca taaccaccaa ctttaccttt atcgccaccg ttgaggtgat
    13261 tgagctcctg gggctgaaga cagtgcttgt tgagcctgat tacggcagtt tcaacatcag
    13321 cgttgaagcc atccgcagag ccatcactcc tcgtaccagg gccattgtgc cggtacatct
    13381 cttcgggcag tgcgccgaca tggaaccgat acttgacctg gcccggcagc atgatattca
    13441 tgtgatagag gatgtggcac aggctaccgg agccgaatac cgcttcagcg acggcaccgt
    13501 taaaaaagcc ggcaccatgg gaactatcgg gtgcacatcc tttttcccct caaagaacct
    13561 cggatgctac ggcgacggcg gtgcactctt cactgataat gacgagctgg ctgcgctgat
    13621 caggagtatt accaaccacg gtatgaaggt gaggtactac catgacacca tcggggtcaa
    13681 ctcccgtctt gacactatcc aggcagccat cctgcgggtc aagctgcggc atcttgacgc
    13741 attcaacgag gcacgtgcca gggcagcagc attctatgac acacagctgt cggggttatc
    13801 cggaatcacc gtgcctgaaa gatctgcctt ctcatcacat atcttccacc agtacactct
    13861 cagaatagcc ggcggcgagc gcaacagtct ccgggagtgc ctagagaacg agggtatacc
    13921 ggcaatgatc tactacccgg tgcctcttca catgcagaaa gcatacctgc acctgggtta
    13981 caggcctgat gacttccctg tcaccacaaa cctctccggc gaggtactct cactgccgat
    14041 gcatacagag cttgacgacg aacagcttgc ttacataacc ggctgcatcc gctcgaaatt
    14101 caactgaaaa catggaaaag gaatattttg cccaccccac cgctgtaata gacgaaggct
    14161 gcactatcgg caacgggact aagatctggc acttcagcca tatcatgaaa aactccgtca
    14221 taggtacgaa ctgcaatata ggccagaacg tggtggtctc tccgggtgtg gttctcggaa
    14281 ataatgtcaa ggtacagaac aatgtatcta tctataccgg agttatatgt gaggatgatg
    14341 tattcctggg accgtctatg gtattcacca acataatcaa cccccgcagc gccgtcatca
    14401 ggcgcgacca gtacgtgacc acaatggtca ggcacggagc ctccataggc gccaacgcca
    14461 ccatagtgtg tggcaatgag atagggcagt atgcgctcat cggggcgggg acagtgatca
    14521 ccaaatctgt taagccatat gccctggtgg tgggaaaccc cggacggcaa accggatggg
    14581 tatccgagta cggacataag ctctcctttg acgaaggagg ccttgccgta tgcccggaga
    14641 gcggccagca atacaggctt tccggaggag aggtgaccag aattatttag tatttttatg
    14701 ccttcaaaaa acattcttat gaaaactgca cccaggaact tcgggctcat agggattgcc
    14761 ggctatatag ccgaacgcca cctgagggcc ataaaggata ccggcaaccg gctgctcgca
    14821 agccttgaca aatttgactg cgtgggaaaa atagacagct atttccccga aagcgacttc
    14881 ttcgttgagt tcgaaagatt cgacaggcac gttgacaaac tgaaaagaag cggcgtgcag
    14941 ctcgattacg tcagcatctg ctcaccaaac taccttcatg actcgcacat caggttcgct
    15001 ctgcgccatc aggctgacgc catctgcgaa aaacccatcg tgctcaaccc gtggaacata
    15061 gatgccctgc aggaaattga gaaggaaacc ggccgtcata tattcaatat ccttcagctc
    15121 aggtatcacc cggtgattat gaacctgcgt gaaagtatca agacgatgcc acaaaacaag
    15181 aagcatgaca ttgacctcac ctatatcaca agccgcggaa actggtacca catatcatgg
    15241 aagggcgacg tcaacaagtc gggcggaata gccaccaata tcggcgtcca cttttttgac
    15301 atgctcggct ggatcttcgg accggtggtc tcaaacaggg tgcaccttct cggacgtgat
    15361 aaggcatcag gatacctgga gctggaaaac gcacgtgtga gatggttcct cagcatcgac
    15421 tggaatgacc tgccggccga tgtgaaggaa aaaggcaaaa gaacttaccg gaatataacc
    15481 gtcgacggtg aggagctgga gttctcggaa ggattcaccg acctccacac agtcacctac
    15541 cgcgaaatcc tcggcggaaa cggttacggc ctggaagatg cacgtcagag cattgaaaca
    15601 gtctatcaga taagaaattc ggcactttcc gtgctgacag gagaatatca cccccttatt
    15661 aaaaaatgaa tatgtacgat aaacttgtaa acagggagac aaagctctct ctcataggcc
    15721 tcggttacgt cggccttcca attgccctcg agtttgcaaa aaagatcagc gtggtgggct
    15781 tcgacatcag gcctgaccgc gttgagctga tgaaaaaggg catagatccc agcaatgaac
    15841 ttgaacacga ggcatttgaa ggaacagata tcaaatttac tgctgatccg caagatcttc
    15901 gcgaagcgtc attccatatc atagccgtcc ccacaccggt gaccaggcac aatctcccgg
    15961 accttgaacc ggtgcttaag gcctccgaaa ccgttggccg catactgaaa aagggcgatt
    16021 acgttgtgta tgaatcaacg gtatacccgg gctgcaccga ggaagagtgc atccccgttc
    16081 tcgagagata ttcagggctg aaatgcccgg ccgacttcaa ggtgggcttt tcgcccgaac
    16141 gcataaaccc gggagacaag aaccataccc ttacgaagat cgtcaaggtg accagcggct
    16201 gtgatgcgga atcaggcgag aacattgcac agacatatga gatgatcatt aaggcaggtg
    16261 ttcaccgcgc ctcttcgata aaagttgccg aagcagccaa gatcattgag aacacccagc
    16321 gtgacataaa catcgccttc atgaacgagc tctccatcat cttcaacagg atgggcatca
    16381 atacatacga ggtccttgaa gcggccggca caaagtggaa tttcctgaag ttctaccccg
    16441 gactggtagg cggccactgc atcggcgtag acccctacta cctctcgtac aaggccaagg
    16501 cgatgggtta tcacccgcag atgattgact ccggaaggtt caccaatgac agcatgggag
    16561 catatgccgg aaaggagaca gtgaagaaac tgatcgcagc tcacaagagc cccgacaggt
    16621 cgcgcgtgct ggttatgggg atgaccttca aggaagatgt cactgacatc cgcaactcga
    16681 aggttattga cgttgtaaat gaactgcgcg actttggtgt ggaggttgat gtcattgacc
    16741 cgggagcctc tcacgcggag gtgaagcacg aatatggcat cgatcttaaa aaaggtcccg
    16801 aaggaaaata tgacgctatc attgttgcgg tgtctcacat ggagtaccgc gacctcgacg
    16861 agaaattctt ccttgacctg ctcaatgaaa aaggtattct tgttgacctg aaggggctgt
    16921 acaggaacaa tatgaagtgg ctgacctact ggagtctgtg atgaacggga aggtaatagt
    16981 aacaggcggc acagggtata tcgggtcaca cactgccgtt gagcttataa ctgccgggtt
    17041 cgaggtggtt atcattgaca acctgtataa ctcagacata tcggtactcg acggtattcg
    17101 gtcaataacg ggtgtcaccc cgaagttcta tgattatgat ctctgtgaca gccagaagct
    17161 ggaagaggtc ttcggtgcag agggaaaggt tgatgccatc attcacttcg cagcctacaa
    17221 ggccgtgggc gagtcagtca ggaaaccgct ggaatactac cggaacaacc tgacctcgct
    17281 gatgaacctg ctggaggcga tggagcgcca taatacaacg gcgctggtct tctcttcgtc
    17341 atgcaccgtc tacggacagc ctgataccct cccggtaacc gagagttccc cggtgaagga
    17401 tgccgcttcg ccgtatggct acaccaagca ggccggagaa gtaatcatac gtgacaccat
    17461 ggcttccaga ccggggcaga aggctatatc cctgagatac tttaatccca tcggcgccca
    17521 cccgacggct ttcatcggtg agcttccgcg aggcgtgcct gaaaacctgg tgccctatat
    17581 cacccagact gccatcgggc tcagggaaga actgaaagtc ttcggaaccg attatgacac
    17641 ccccgacgga tcatgcatcc gcgactacct ccatgtggtt gacctggcga aggctcatgt
    17701 ggtggccctg aagagattgc tcggcggaga aaacaaggca aactacgagg tcttcaacct
    17761 tggaatgggc aagggtgtct ccgtgctgga ggcaattgcc gccttcggaa aagccaccgg
    17821 ggtcaggctg aattactccg tcaccggccg cagggcaggt gacatcgaaa agatctgggc
    17881 agatccctcc ctggccaaca gggaactggg ctggaaggcc gggataagta ttgaggaggc
    17941 aatggagaca gcctggcgct gggaaaacaa actgaaaaac aagaaatcat gaacagaacg
    18001 atactcatta ccggcggcgc aggattcatc ggctcacacg tcgtaagact ctttgtgaac
    18061 aaatacccgg aatataagat cgtcaatgcc gacaatctta cctatgcggg aaacctgggc
    18121 aatctcacag atatcggaaa caggccgaat tatctcttcc gtaaggttga cattgttgac
    18181 aagaaagctg taaatgcact ttttgacgag ttcaggtttg acggagtgat acacctggca
    18241 gctgaatcac atgtagaccg gtcaataacc tcgcctgacg agttcattca tacgaatatt
    18301 ctgggcacgg tcaacctgct ggctgcattc agggatgttg ttccggcctc aggtgctgac
    18361 aaacggtttt accacatctc cactgatgag gtttacggct cgctggagca cggaggattc
    18421 tttaccgaac agactcccta tgacccgcgc agtccctatt cggcctccaa ggccagttct
    18481 gatcatatgg tgcgggcgtt tggcaatacg tacaagctgc catttgtgat ctccaactgc
    18541 tccaacaact acgggccaaa ccaattcccg gaaaagctta taccactgac gatcaataac
    18601 ataaagacca tgaagcccat cccggtttac gggaaagggg agaacattcg tgactggctt
    18661 tatgttgagg atcacgcctc ggcgatagac gttattttcc ataggggcaa gccgggagag
    18721 acctataacg tggggggtga caatgagtgg aagaacatag atctcgtaag gctgctgtgc
    18781 aggatcatgg accgcaagct gggcagaaat gacggcgact cggaaaaact gatcacattt
    18841 gtgactgacc gtgcggggca tgacctccgt tatgccatcg acagcagcaa gctgcagcgt
    18901 gagcttggat ggaagccgtc agtcaccttc gagcaggggc ttgggatgac cgttgactgg
    18961 tacctatcga atgagaaatg gctcacagcc atcatctcgg gagactacgc gaagtattac
    19021 gaggaaatgt acggaagcag gtaggagttg cgatctgaga agtgcgaatt gcgaagtgcg
    19081 aggtctcggg gccttcagac tccagacctt cagactccag accttcagac tccagacctt
    19141 cagacttcag accttatgac cttaagacgt cctatcagct catctcccag ctccgtcttg
    19201 gcctttatat gatcgaggat ggcaagcacc acgccgttct tttcaaaatc tcccctcacc
    19261 tgctcgaagt cgcgcttctt gacctcatcg tcgccgtcaa tgccgaagcc ggtcattgac
    19321 gagagggtga attccttgcg ggcatcctca taaagcatcc ggtcaaggtc aaccacgctc
    19381 ttcttccatc tctccacgat tgcaattata tcatcagcgg tcagttcttc gttgggtttg
    19441 ccggtctccc ttggcagatg atcgtaaacc catgtccact ccaggttata atagtgcttg
    19501 tgaatattct cgaagctccg gttgatggcg tcggcgctct tcatttcctt cctctcgatt
    19561 gctgccagga gctcctcaat agagctgagc ggtgtgatca gtccagacat gtctatccag
    19621 tctccggtgc cgccattggt atccggtttc atcctgtcac ggatctccgc atcagacttg
    19681 aaaacggtcc cctcaagcct cttgatcacc gagtttccta taaacttgtc gattcccatc
    19741 tggtagaggg agatgcccct cccggcagcg gagattttca tcctcatcct ttcataggtg
    19801 atgatctcct ggccgtgatt tgcatcaata agtaccttca ggagatcccg tcccttcatc
    19861 atcttcccca cagtaaaggg gctgagaagg ttgtagttga tctggtcgag cttcttcgtg
    19921 tccttgcgca tatcacgttt cggccatttc tgggcgtcgc ggatggtacc caccgagcgc
    19981 aggttgattc ccgggatcag ctgtgactca tagcttacct cggtaaggta tgagaacggg
    20041 aaacttgttg tgtcaacgtt atggtagtgc cggcccacca ccagggtgaa cggtcctatt
    20101 cgcgagggcc acagcacata tgaatctgat gtggtcttcg agcctctctc catgataccc
    20161 tggtgtatcg gtcccagctt gtacatatgg ttgctctggt tggagccgct gcctgcgttc
    20221 atgaatgaga agatgcctgc aatcaggaga gtcgatttgt gatgggttac cgtatagggt
    20281 cctgcaaaga tggagcatgc ctctccgtga taccctccgc agttcgagaa gaacagggag
    20341 ttctcggctg aatactgttt tgagagggtg catccctgac cgatgaagca cttgtcaatg
    20401 actgcggcgt cattcacctt tgatccggag cagatgatga agtgatccat tatcacgtca
    20461 ttgcctatgt acacgggatc cctgaatgaa ctgttgacgg atccctcggc cagccttgag
    20521 acatcctcta tcacggctcc gttgcctatc ctgacgttct tgattgtgcc gcagccggcg
    20581 atgtatgatc ctgagccgat ttcgccgcgg tctgatttga cgctttctgc atagtcatct
    20641 atgagcttct cgatctcggc gatggctgaa ggtctgtgcc tgtacagtgt aatgatatag
    20701 gccatgtgtg atgtcagccg gtcccagatg gggacctccc tgccgccggt ctcattgaga
    20761 actgccacac gggttccgtt tccgaaagag gtgtgcccct cggttgaaat atgtccgcaa
    20821 ttcctgataa ccaccccctc accgatgtca tagttggcga taaagcctgt gatatcagtg
    20881 atagtgacgt tgtcagctat tgtgcaattg tgtatgtgtg cgttttttat gccgtgcatc
    20941 agtggcagtc cgcatctttc gcgggccacg ccgttgaaga ggcccagcct tacggtaccg
    21001 gagaaggtga cattctgaat cctctctgca ttgaacggat ccttgacaaa tacactttcc
    21061 catgagttgc tcctgcagcc tgctgcctcg aggccgcgaa tttctcctat gctcagactt
    21121 ctgtaaccgt cagccattat tcccagcttt aattattcta cagtaactga ttttgcaagg
    21181 ttcctcggct ggtcaacatc gcatccgcga aggacagcta tatggtatga gagcagctgg
    21241 aggggcacca cggcaagcac cggggcgaag actccgatgg tctccggaac ttccatcaca
    21301 tgatcagcca tgtggcttat gacctggtcg ccttctgtca caacggctat gatctggccc
    21361 ttgcgggcct tgacctcctg gatattgctg acgatcttgt cgtatgagtt ctccttcgtg
    21421 gctacaacca ccacgggcat atgttcatct atcagggcaa tgggcccgtg cttcatctcg
    21481 gcagcgggat agccctcggc atgtatgtac gaaatttcct tgagcttgag ggcgccttcc
    21541 atggccacag gatagagcag gccccttcca aggtacagtg cgttggttga gtccttgtat
    21601 atctttgcca gggccagcgc cttgtcattc atcttcagtg caatgtccac cttgccgggc
    21661 aatgaagtca gttcctggat cagtccgcgg taacgcacct catcaatcag tcccttcctg
    21721 tagcctatct cgaaggcaac cattgccagt gtcaccacct gggtggtaaa agctttcgtt
    21781 gatgcaacgc caatctccgg tccggcatgg gtaaagacgc cggcgttggt ctcacgggag
    21841 attgatgagc ccacaacgtt gcagatgcca agcaccagtg cgccccgctc ctttgcaagc
    21901 cggatggctg ccagtgtgtc ggctgtctcg ccgctctggc tgatggccag tacaatgtct
    21961 cccttcttta tgaccggatc acggtacctg aactcggagg catattcaac ctcaacgggt
    22021 atgcggcaat attcctcgat gatgtattct cccacaaggc cggcgtgcca tgatgtgccg
    22081 caggccacta tcagtaccct gtcggccgta gccattgtgt caaacacgtt gtgcagcccg
    22141 ccgagcatga gacctgactg gtttgggagt acccttcccc tgaaggtgtc atgtatagcc
    22201 cgcggctgct cgaatatctc cttgagcatg aaatgaggga agccctcctt gtcaatctcg
    22261 ccaagcttaa ggtctacctc ctttacttcg ggttcaaccc tcttgttcct gatagccttg
    22321 agcaccatct catccttcct gatcaccgcg aggtcatcgt cgttgagata gattacccgc
    22381 tgggtatgct cgacaatggg agtggcatcg gaggcaatga agttctcacc gtcacccaca
    22441 cctatcacca gtggtgagcc cagccttgcg gcgaagagtt tgccgggttc atcacggcag
    22501 tagacggcaa tgccgtatgt tccctccacc ttgttcaggg ccatgattat agcctgctca
    22561 gccgagagat caccctcaag gtagagatgc tccataaggt tggcaagcac ctccgtgtca
    22621 gtctgactgg taaaactcac atcacggctc tcaagatgct tcttgatccg tgcatagttc
    22681 tcgataatac cgttgtggac cactatgaaa tgacccctgc aggagacctg aggatgagcg
    22741 tttatctcgt tgggctcacc gtgggtggcc cacctggtat gccccatggc gatggagccg
    22801 tcgaaatcct tgttgcgcac catctcctca agctcgcgga ccctccccgc acacttgaat
    22861 atctctgttc tttcgccatt gctgatggca atcccggctg agtcataacc cctgtactcc
    22921 agccttttaa gtccgtttat aacaatatca cgggcaggac gggatcctat gtatcctaca
    22981 attccacaca taaagaaaaa ctaaatatta gaactcaata tatcgtgtaa gcaaactcca
    23041 gcttaaatgc cggctcgttt gcgttagccc tgaagacagc attccgggtt gaagtgaggg
    23101 gaagaaagag ttccactgat ggttcctcta tctcaccatc gaggtatttc tggacgaagg
    23161 tggttatgtt gaagatatag tagtcattgt cactgtagta ggtgccatcc ataaagctga
    23221 tatcatgcag ataatcaggg ataacctgga ctttcccgtc ggcgtcgcga taacgtacat
    23281 ataccctctg tggcatggtc ttgtcaagga ggatctcacc gtcaagaatt accggggcaa
    23341 taatccttgc cttattgacg gcaaggttgt caacatccct gaaaggctcc agggttggca
    23401 tgtcaagctt cacgtataca ccattgtatg tctgaacgaa gacggcagtg tcagctacaa
    23461 ggtcattgac atgaagtatc tgtttggcag ggtcagctgt tgaacggtca tgggtgaacc
    23521 tgttgtagtt cactgccctg ggagtagcca cgaatgaata agagtgcctg agtgtgtcag
    23581 atttatagaa gatggttata cccagcgggt caacggatgc cctctctgat gcatccataa
    23641 caaccagaac cgggttgggc gagcttttga tcatgcagta gagtcccttg aaataatcct
    23701 cgtagaatgg agctcccctg gataatttag tggtgtctct cagcaggtat tcgccgaccg
    23761 agttatctag ctttacctta agaggtgtgc cggctttcat tgttggaagc acgtactcgc
    23821 cgaggaattt gattgtgtcc gggtcctgac ctgcatagta ttcagcagtg tctgtgagct
    23881 tcgtcccggt ttcgcacagg cgtatccggt gaactgatgt ggaatcatca cccgaaacat
    23941 ccgaagggaa catggtcagc aggacagagt cgataacata cggtttggca ggccacttgt
    24001 ctgtaagcct gagctgtgtc acgaagtcac agtatgttgt gccgaaatac tcattataga
    24061 gtgacccgat gatcattgtc gtgtagtcac tcgagacgga gagttcgtca tacatggtat
    24121 aagccttgat tgagacagtg tcagttgagc ctatatctat gaaatcactg tcaggcagga
    24181 ggtcgatgcc tatcttgacg ggatcctgtg agcacgacag caatgctgcg gtgagcaggg
    24241 cggagagggt cagtatgatt gaaggcgctc ccttgcgggt attgccgggt ttgttatttt
    24301 gcagattcca gaaggagttt gtcataaaag tcattgtatg catctatata attctcctct
    24361 ccctggtact caagcagagg ctttcccatg gcagtgatcc ttttctttac ctgttcgtca
    24421 atcttctcgc acccgaaaat aattccgtca gagttctcgg cggcaagggc cgtcaggttg
    24481 agaaaatcag cgcttccttt tatagacttg agagtgccgg tatcaatccc gtctgccagt
    24541 agcttttcgg acagagaaga ttttaacggc tttttaaatc cgttattgta aatcgaatag
    24601 actgatttga ccttgctgaa taccgggtca tcggaataga gtttcctgag atagaggggc
    24661 accagggatg cgagccagcc gtggacgtgc actatgtcag ggctccagcg cagttttctt
    24721 accgtttcaa taactccacg ggcaaagaaa atagctcttt cgtcattgtc ttcgtactca
    24781 ttgcctgcgg catcagccac agtatgcttg cgctgaaaat aatcatcatt gtcaatgaag
    24841 tacacctgca tccgggcact ctgaatggag gctactttaa taataagagg atggtcggtg
    24901 tcatcaatga tcaggttcat cccggagagg cggattacct catgcaactg gtttcttctc
    24961 tcattgattg agccatatcg gggcatgaat gtacgtatct ccttcccccg ttcctgtatg
    25021 ccctgcggca gatcacggct gatctttgaa agcttcgttt caccgagata gggcataatc
    25081 tcctgtgaaa caaacaatac ccttctgctc tccattaata cctcccgata tacctaagga
    25141 tttatcaaag tgcaaagata ataaaaaaaa ctcaattaat ttccaagctt agagacaacg
    25201 ggttactgac aaaaatggtg ttattttgca gtaccggaca ctatcggggt gccggaaaac
    25261 gatccggcgt tcagacaaaa agcattaatt atgaaagtac tgacaactgc caaggaagta
    25321 agggaacact acaggatcag cgtcctgcgc aacccgggct tcgtgcctac catgggcgca
    25381 ctccatgagg gtcatctttc actggtggca agagcagcgg agagatgccg tatggtggcc
    25441 gtgagtatat tcgtcaatcc cacccagttc aatgacccgc aggacctgct caaatacccc
    25501 agaacacttg atgccgacct ggagatgctc tcgggcctgc taagggaaaa tgactttgtc
    25561 tttgtaccct cagtggagga gatataccct gagcctgata cacgccagtt cgacttcggt
    25621 gagctggaaa aggtgatgga gggccttcac aggcccggcc atttcaacgg cgtagcccag
    25681 gtggtctcac ggctctttga cattatcagg ccgtcatacg cctttttcgg gcagaaggac
    25741 ttccagcagc tgacaatcat cagggaactc gcacggcgcg aatacccgaa ggtcgaaatc
    25801 atcgcctgcc ccattgtcag ggaacatgac gggctggcca tgagcagccg caatcgcagg
    25861 ctcctgacac agcaccgcga gaaagccggc gaaatatacc gtaccctgat cgctgcagca
    25921 gccatgatct ctgactatga aataaatgag atcagggagt tcgtggctga ccgcataaac
    25981 atgatccctg acttcaggct tgagtacttc gagatagtgg aggaaggcac actgaaaccg
    26041 gttcattcgg ttattgaaat gtcaccggaa aagaaatatt tcgggtgcat tgccctgtgg
    26101 gccggagaaa taagactgat tgacaatatt gagataggat tgcggtaaat caaaggataa
    26161 ttatctttgc accttcagaa aacaatctgg ttcataaaat gacaatagaa gtactcaaat
    26221 caaaaataca taatgtcacc gtcaccggtg ctgacctcaa ctacgtgggc agcgtcacca
    26281 tcgatgaaga cctgatggat gccgcc
//
LOCUS       DAOPXU010000031        26166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_1851_length_26166_cov_12.914404, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000031 DAOPXU010000000
VERSION     DAOPXU010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 26166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26166
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1851_length_26166_cov_12.914404"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(391..1326)
                     /locus_tag="PKK03_04760"
     CDS             complement(391..1326)
                     /locus_tag="PKK03_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83739.1"
                     /translation="MKTKAIHFTSFGNAVIVQISSQPLSAFPFLPAGPAIRNGSLIIS
                     ELTASGSVGQLAAINQTSDYLLLTDADVLTGAKQNRVVNRSVLLAPSSKTVIEVSCVE
                     RLRWDYRSSSFSTPGSVADPNLRGRKAGTFSRMKPENEPGFCNTQGEVWDHVRANLAC
                     EGVHSRTESYEELLQVRMQKADRVFPECHPAEGCNGLAVILDGKVISADIFGNEDSFR
                     YYFPMLCDAAFTLTRPGDDKEQPEMHEAYFRALEAIDEFNDAGRHTEDAHPGAGTLSM
                     VETERLVGFSLVHGDEMIHNALFARHDPATRLFAE"
     gene            1422..2075
                     /locus_tag="PKK03_04765"
     CDS             1422..2075
                     /locus_tag="PKK03_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83740.1"
                     /translation="MPANLNALIRYKTINSCLAGGRRRWSIDELRARCSEALASDRGR
                     YATVSERTLRDDIRVMRSDILGFNAPIKQESGFYFYSDPGYSILSLRITDAGLAEQIL
                     GLLMKMREKVNHPEMEIIMEKLSRLIGREYGPESGLVVRNQVNDEAIKIKKQVIEEEI
                     NANERMSADLENRGIFERRRLRLMLRKMDAPPIVPPSELIWGEIMAAVMSGIFRRQP"
     gene            complement(2072..2362)
                     /locus_tag="PKK03_04770"
     CDS             complement(2072..2362)
                     /locus_tag="PKK03_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83741.1"
                     /translation="MDGVGFTWAPDEGLRIGPTYRIDYHIIEGKARYPSYFVVKDGGE
                     MIKVEKENFDPLFEQLFGDCPAMQEELAMNPDLKKFKNFMILAEVYNRLCAL"
     gene            complement(2538..4247)
                     /locus_tag="PKK03_04775"
     CDS             complement(2538..4247)
                     /locus_tag="PKK03_04775"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNX83742.1"
                     /translation="MKRKLAEPGILFLCILLLAGCEDAERGPKDYPFVQMDNVDVLSD
                     GVTFQARITSPGTQNIVSYGFVWNETDNTEFETWSEKELTGILSGGTFQAEVHTDIVK
                     DQVYHVRPFVKTVETTVFGPVTMFTGRGSRPARVTDYSPKEGFDGTVVKMYGSNFSQK
                     QGNLSVFVDIAPAEIIISSEDSLVFKIPETPLAGRVRLKVVSGDREIIIENAFEIFGP
                     EIFESSVTEGYAGDIITIRGKNLTHYGPPTLHFDAFVAETIESEEDQVTAVIPFVNNF
                     YHDKAVSVSFFSGQKFGGLNTPFTVKKSWDTKTPTPFDWTWTYDAFSYNGKGYILEIN
                     AKLLYEYDPVADSWDPVSDFPGERSENNIFLPHDDKFIVIGGNTDGASCPVWEYDFTT
                     KIWTRQDDAPFIFTLATHVTLNGLEYIITDSGLVCTYNYSTRGFTKKYHFLHPVNLFL
                     FAYIANGKIHLVTYGSTWSYDEGTDSWTKVSDNQFEKDYYYVPAIGFYYSGSPYVLED
                     GVELYKYLADYDRWVLCGYYPGCRGDNSYKTVFVIGDYAYVAATTSNYDGCAPLHFAY
                     RDR"
     gene            complement(4250..5365)
                     /locus_tag="PKK03_04780"
     CDS             complement(4250..5365)
                     /locus_tag="PKK03_04780"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX83743.1"
                     /translation="MKNTKIRQIILILLPLAQPVYSQNNNYIQADSVMTIGIDLIKGL
                     PNQNSQFVAVKRKAGEIRYLPYQLTGYGFRGGPEYVSRSIIVNGQPERVFLERISKGR
                     INLYYYTEKGLKTYFLEKDSTVFAEIPDRYDFRAPILEHTSDFNWKASQVQLARYHRN
                     SLKKLISMYNKGINRPLPFPRFGVAAGYSVMNLAVPVGLNVGEAPFLSFSPASSPQVG
                     IFADLPVLVSDFSVNTGVYLSKHGFSANSVTSMADIDVVVNLISARLPVLLRYTVPTL
                     TWRPFINAGGIGSWHLKYKREIYESTIRENSIIINEVESSSVSSRVRFGYSAGAGMQY
                     NLNNRNAASLEVRYSQFPATLRESDMSFTEVLLSYSF"
     gene            complement(5486..6940)
                     /locus_tag="PKK03_04785"
     CDS             complement(5486..6940)
                     /locus_tag="PKK03_04785"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNX83744.1"
                     /translation="MKRYYAIAILVFMSITGLSQSPRMDYSEAFKLIEVWLDAQRDYE
                     RLPALTAAVVHDQEVLWAGAFGLANAEDSVKAEPSTIFSICSISKLFTSVAIMKLYDE
                     GKLRLDDEIGDLLPWYDLKQKYPESGPITVRSLLTHSSGLPREADIPYWTAPDFRFPS
                     RDQVKAALGNQETLYPSSTIFQYSNLGMSLLGEVVEQVSGMPYDEYMERNILGPLGLN
                     ETGTSLPEACHGTRMAVGYSAVNRNLERKEVNFFQANAIKPAAGFSSTVLDLAKFASW
                     QFRLMDTTSEEVLKPSTLKYMHNVHWTDPDWNITWGLGFVVTRGEDGNTWIGHGGECP
                     GYRTLLQLCPKTKLAISIMINANNTNPDKYAGAINAILAGAVATTPRDSGIPGKKNAD
                     LQDYVGYYSDQPATSELYISTWGGDLILLTLPNDDPAGSMVLYRQVGKDTFRRVGNDG
                     ELRETLVFERDTDGRVFRYKTFENYVIRISRKQA"
     gene            complement(7128..7262)
                     /locus_tag="PKK03_04790"
     CDS             complement(7128..7262)
                     /locus_tag="PKK03_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83745.1"
                     /translation="MKAYFTYDTDFFVRESREDMKFILDPAGKVTGFSAGGTLVRKVN
                     "
     gene            complement(7259..8038)
                     /locus_tag="PKK03_04795"
     CDS             complement(7259..8038)
                     /locus_tag="PKK03_04795"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNX83746.1"
                     /translation="MKRNLICLPAVLLPLLLITGCQPGTQSRYSPETEERIKLVENNL
                     GDWVKGYGWADVDEKRPVTEKTLFQAASISKSLNGVGVLKLVQDGKLDLYTDINQYLK
                     SWKFPYDSVSGGKPITLSALLSHTAGLTIHGFPGYAQGDTLPSVQQVVDGQKPANTEA
                     VRSFMEPGTRVVYSGGGTTITQQIVTDVTGRPYDEYMQKNVIDPMGMKSSSYRQPPAG
                     TDTSLLATGYKADGTRVDGKFHIYPEQAAAGLWTNPTDLSG"
     gene            complement(8150..8716)
                     /locus_tag="PKK03_04800"
     CDS             complement(8150..8716)
                     /locus_tag="PKK03_04800"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNX83747.1"
                     /translation="MIVEPTVITGLIIVIPILLTRYLLTSLLSKEAVRRAAFFPPTRG
                     LEKPAYLVNIGTTLLLLVIPFFLRIKLNGFMGIAGVCLSLAALVLYGMSIVQFARPDA
                     GGINRSGLYAVSRNPMYVAFFLYFLGACMVTRSWLLLAILLVFQVSVHFMILAEERWC
                     RETFGETFDKYMEKVSRYGLGHYKSILH"
     gene            complement(8713..9072)
                     /locus_tag="PKK03_04805"
     CDS             complement(8713..9072)
                     /locus_tag="PKK03_04805"
                     /inference="COORDINATES: protein motif:HMM:NF025161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HNX83748.1"
                     /translation="MIKYHKTAKGISIAEILPGSETIGTTEDLLDILADAGYNGSTGL
                     IIHSATLTGSFFDLKTGIAGEILQKFSNYRMRLAIIGDFANVRSKALRDFIRESNTRG
                     TINFVETAEEALSRLEK"
     gene            complement(9093..9632)
                     /locus_tag="PKK03_04810"
     CDS             complement(9093..9632)
                     /locus_tag="PKK03_04810"
                     /inference="COORDINATES: protein motif:HMM:NF027625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4943 family protein"
                     /protein_id="HNX83749.1"
                     /translation="MKSIVILTLIALLCPGCDKGSFDFNNPDVTGFVRQLENGTYNQY
                     EYTEDGERLWTKMPAFQKKDIPELISLAKETGLISPCDHFPVNPISSMWPFRVVDGRE
                     CIMLGEYLLWCAEAVIEGKDFASLNPVLYNKDYPNDQRLDAAEIMAVRKLYQEWWDEY
                     GHQADPEKLPLEGTVYIWR"
     gene            complement(9666..9827)
                     /locus_tag="PKK03_04815"
     CDS             complement(9666..9827)
                     /locus_tag="PKK03_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83750.1"
                     /translation="MEQRDYLLREIEKTGAIIMAIRRKLFGRTDEPAISVENQAEALQ
                     DDRPEERNH"
     gene            complement(9843..10364)
                     /locus_tag="PKK03_04820"
     CDS             complement(9843..10364)
                     /locus_tag="PKK03_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83751.1"
                     /translation="MKGRILTVMITALVTIILIAVAVYLIFGFSSKPGKIMSVNSPQG
                     SFEAYVVESPSADPPKQSLFIAKAGTGEFRLVASLPEDIESAHKIYWTEDGNKAIFVT
                     SWHLFVTDVQTFNTMKISLNPDWWKMHEGKKTFSSSGTSVVMEELILNGSDSLTYRTN
                     LMTQPVTVSLTGL"
     gene            complement(10381..10806)
                     /locus_tag="PKK03_04825"
     CDS             complement(10381..10806)
                     /locus_tag="PKK03_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNX83752.1"
                     /translation="MAELKTKKSGSDVNEFINSFADTEQKRKDSFELLKLMREFTGFE
                     PKMWGASMIGFGQYHYKSERSSQEGDWPLVGFSPRKAAISLYVYTGAPEHEHLLEGLG
                     KFKKGAACIYVKKLSDIDQEALKKLMKTTIGFLQARHGK"
     gene            complement(10919..11830)
                     /locus_tag="PKK03_04830"
     CDS             complement(10919..11830)
                     /locus_tag="PKK03_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX83753.1"
                     /translation="MKKSLLFLSALLLGVVVCAQERHIITTDGVDLYVNVKGEGTPCL
                     YLHGGPGSGSYWLEKFAGDSLEKHFRMIYLDQRGVGRSSSPADGNYSMDRMVKDFEEV
                     REALGISRWITLGHSFGGLLQMGYSLRHPPAVEAMVMINCSLNMGLNYKTSWCPKACE
                     LLGITDRSYFVDESIPLHVRWDSLITALNSSDMMWKMGFASREEMNIMNSTYGELPSW
                     NGDFSEAAMRMDDYGEDFTEATAKVNMPVLFIYSTRDWMIGPEHYKTAQFPEMMLVSI
                     DAAHMPFFGDNAAVMKAISTFRKRYRL"
     gene            12064..13425
                     /locus_tag="PKK03_04835"
     CDS             12064..13425
                     /locus_tag="PKK03_04835"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HNX83754.1"
                     /translation="MKKHFKLLGAAVIIGVALGSCETDEPIDESEFGDLKIAYNGLIK
                     PTDNSIVLKWNEAVSKVVDNKMPPPPEARIYAMVMLAMHDALNQVIPYYETYALDNSG
                     VNTKGITRKVIQGLANAAASQAAHDVLVALVPATERDAGILLEACLEEVEASEYRDMA
                     IDAGARAAAAMLLKRSGDLPLIFQAYPQGTEPGQYRSPGNFANPGPNWPANAAYAPDL
                     GELEPFGVLSSDQFRAIPPYSVGSFEYADEYNEVKLLGSNSSTARTPEQAEMGLFFLD
                     NVASSINRVVRVVAVKFNLDCWETARLLALVHMAQFDAVQSSFEGKYHYNLWRPETAI
                     RLGELDGNGATTGDAGWSILSGARNTPPTPTYPSTHAEAAGAVTEILHLYFKRDNVQY
                     KIGSYSLPGVERSFSSFSQFASECAESRIYIGYHFRNDVIHGEMKGRELARFVFANNL
                     RAL"
     gene            complement(13494..14219)
                     /locus_tag="PKK03_04840"
     CDS             complement(13494..14219)
                     /locus_tag="PKK03_04840"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNX83755.1"
                     /translation="MQKWKCLIVDDEPPAARVLRNYIESLDYLEVAGTCSNAFEAIEM
                     LNSMKVDLMFLDISIPKMLGTQMVRTIRTTPRVIFITAHRDYAAEAFELDAVDYLMKP
                     VSFERFMKAVNKFRQVCTPDPELNDHSPGFLYFRTERKMVKVFLDDITYIESFRDYII
                     IHRQTAADLKVKCGISSVESMLPHNRFLRIHRSFIVSVPKVTAFTTNDVEIGTRELPI
                     GRSYHEVHEKLTSGNAVFRGIEL"
     gene            complement(14191..15261)
                     /locus_tag="PKK03_04845"
     CDS             complement(14191..15261)
                     /locus_tag="PKK03_04845"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNX83756.1"
                     /translation="MKFRRIVFPSIVRTVLLHILFWIMTVIYFAWGFGLNVNPGRSLI
                     NSALYLPGFFLMVYTLLYVLVPRFLLKKKLFSFFAGLGIVLAICMIYSNLAQLGLDAS
                     APLKGMTLKVGRNVLPYLHVAGIALSVRMLMYWYEQREQTLEAEQKKTAVELQLLKAQ
                     LHPHFLFNTLNNLYSHTLEASPRSPEIVLKLSELLRSMIYDSNAPKIPLAKEIGLLRN
                     YITLEQIRYGDRLEISVTVTGEAGRYQIAPFLLLPFLENAFKHGTGKQLGRCWITIDI
                     EVAGPVMYFKLVNSADPEKDEEPANTGGLGLANVRRRLELMYADRHRLLTTRLKETFV
                     VDLVMTLEKTEERYAEMEMSYC"
     gene            complement(15436..16377)
                     /locus_tag="PKK03_04850"
     CDS             complement(15436..16377)
                     /locus_tag="PKK03_04850"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNX83757.1"
                     /translation="MDEPKTSRSEYISRINKTLDYIDSNIENLMTLGELASVANFSKY
                     HFNRIFHSIVGETPFQFIQRVKIEKAAMLLLTNRKMTVSGIATKCGFSDLSVFSRNFK
                     SCFGVSPTSYRANKPQNSNLSQTVSKGSQAEAKAVPYFCPELQTIKWRTNMKLNKSVE
                     VKELPKMTLAYIRHIGPYKGDDKLFEGLWNSLFAWAGPRGLIGGKGFRSLVIYHDDPG
                     VTIEDKLRMSVCITVPPETKVDGEIGKMELEAAKYVIARFEVNAQQFQEAWDWLYGEW
                     FPASGYQPDEKPCFEMYPEEPRDGKFVVDICVPVKPI"
     gene            complement(16402..16833)
                     /locus_tag="PKK03_04855"
     CDS             complement(16402..16833)
                     /locus_tag="PKK03_04855"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNX83758.1"
                     /translation="MDKSKELTATVRLINDKLNFSGTVEGNIPVSIDYTPPLGDNLGY
                     TSLELLLLSLSSCLGSATLTFLRRMGKTITGFEIRSKGFRKPEHPTGFSNIIMEMDIT
                     SDNLHEAEVDKVVKLAEATYCPVWSMLRGNVTVEVKYNFMK"
     gene            complement(16871..17326)
                     /locus_tag="PKK03_04860"
     CDS             complement(16871..17326)
                     /locus_tag="PKK03_04860"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNX83759.1"
                     /translation="MKLKILVGSGRKIGLFTLPFLVAGVALNLLFPQFFRVGGPTDTL
                     KWLSAAILVPGVIIWIWSVVLILVKVPRHELITTGPYALVLHPLYIGVALLVLPWFGF
                     LLNTWLGLLIGLVVYTGSRLYAPEEEKILAKIFGERWDSYRKKVLLPWL"
     gene            complement(17337..18005)
                     /locus_tag="PKK03_04865"
     CDS             complement(17337..18005)
                     /locus_tag="PKK03_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNX83760.1"
                     /translation="METRIKICCIHDIREAETAMRYGASAIGLVAAMPSGPGPIPDEL
                     IRQIAATTPPPIATFLLTSRTSVREIIEHHRLTHTNTIQIVDALSEGTYAELKSALPG
                     VKIVQVVHVLREESVEEAVRISRSVDAILLDSGNPNLRVKELGGTGRVHNWQLSRRIR
                     EQASCPVFLAGGLKPENVREAIETVEPFAVDVCSGVRTNGLLDAEKLGRFMSEVRAAD
                     RTIH"
     gene            complement(18020..18514)
                     /locus_tag="PKK03_04870"
     CDS             complement(18020..18514)
                     /locus_tag="PKK03_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005807130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX83761.1"
                     /translation="MEAEIIIRDLDQSETGILKTMLYNAIFIPPEEVKPPPEIIELPE
                     IAVYIKDFGREGDICLVAEIEGVIAGAVWTRLFTAGAQGYGFIAPEIPELSMSVLEQY
                     RRQGIGTMLLAGMLKKLASKGFRQVSLSVDLANYAYRMYRKQGFEDYARHDESMTMLR
                     QLKP"
     gene            complement(18581..19570)
                     /locus_tag="PKK03_04875"
     CDS             complement(18581..19570)
                     /locus_tag="PKK03_04875"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNX83762.1"
                     /translation="MSQGAKQPDHANHGDDYSYAIKSALFGVAVGDALGVPVEFKSRD
                     YMNLHPVTGMTGFGVHNQPPGTWSDDSSLTFCLAEALTQGYDLSAIGQSFVNWYFRDY
                     WTAGGKVFDIGTSTGEAIVRIFKGTNPELSGDTAVSSNGNGSLMRILPLMFRLLPRPL
                     TERFEITRQVSSVTHGHIRSIISCFYYLEFARQILEGREKYDIYRDLQKIVSGHLSAL
                     LIDPAEVVVFDRLLKYDIFRLNENEIRSGGYVIDTLEAAVWCLLTTGSYSEAVLRAVN
                     LGSDTDTTAAVTGGLAGLLYGFDTIPDSWVKQIARRKDIEELAEKLAVGLNGN"
     gene            19745..20155
                     /locus_tag="PKK03_04880"
     CDS             19745..20155
                     /locus_tag="PKK03_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83763.1"
                     /translation="MKILRGTLFGGIAYFLIGWLVYGVLLYNFFAPMTNTCANRPEGE
                     MIWWAMILSNLLIALFLTLFLKWSGARGIVDGLKTGALFGALFTATIDLSLFSMTTMY
                     NSLVPVVLETLASAVVMAIVGMIIVLTWGRPKEI"
     gene            20165..21112
                     /locus_tag="PKK03_04885"
     CDS             20165..21112
                     /locus_tag="PKK03_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNX83764.1"
                     /translation="MKAVVYNREAKPYRLVLREVEKPFPKDDEVLVSIRAASVNAADY
                     RSMKMGMIPARKIFGADISGVVESAGKNVKLFRPGDEVVGDLSGCGFGGFADFALATE
                     KALVHKPAGLSFEMAAALPMASMTALQALRNKGQIQKGQEVCILGSSGGVGTIAIQLA
                     RHFGAVITAVCSTQNVEQSQLLGADRVIDYSKEDFAVGNRKYDLIIAVNGNYSLLKCR
                     KALKADGRYVLVGGALSQIFKAIVIGKPLSLGRKKMLFLAAKTDTNDLRYLLGLASDG
                     IIKPVIEKYFSPETTAEAMKYAAGGHARGKVVIKWNPEA"
     gene            complement(21227..21841)
                     /locus_tag="PKK03_04890"
     CDS             complement(21227..21841)
                     /locus_tag="PKK03_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNX83765.1"
                     /translation="MIFVFLAVAMLLLAASCSRRAVPADSGNGNSGRSAGGVLIKTPE
                     FSLRIPDVSISPSETKRLEQFMNAGIERSVGTGTVSPAAIIGTAREYLGVPHCMGGTT
                     EKCTDCSGLIFRVFATHGIILPHNSEEQARYGRIITERELLMPGDLVFFVRTYNTSRL
                     ITHSGIFIGEGRFIHTSSSQGVTITPLDDPYWKDRYLFGTRIFE"
     gene            complement(21904..22848)
                     /locus_tag="PKK03_04895"
     CDS             complement(21904..22848)
                     /locus_tag="PKK03_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83766.1"
                     /translation="MKKIALLLALTAVATGLFCQVDTTSVSYVAYWSTGDSYDFRVHK
                     TSMQWVGDSLTKNDISEYTANFEVVDSTDTSYTVVWTFSNELINTWDIPDELLEELSK
                     YSVTEVVYTTTELGEFNGIENWKELGEMMRSMFSDVFRVISKDSGEEYEKLMQTARPI
                     IDMYSSKEGVEQMAFPELMLFHFPFGAMIPVADTISYEDLFPNLLGGDPIRADAKLWF
                     EEVDFEEERSVLVQESKLNRDDTMAFLRSIFDRMGVEDASLTEALAMAVYEINISNRY
                     DYYYYPGVPVNIEFKRNMTIDVAGQRTRRNDTITIELL"
     gene            23019..23633
                     /locus_tag="PKK03_04900"
     CDS             23019..23633
                     /locus_tag="PKK03_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83767.1"
                     /translation="MKKNAILLVGLFALLTLFSCSKNNDPVNSFTFDGDEYLINDLYL
                     IEEVFNKGASNEMHVFQFMFGNIENGDTTMLALAVLDKNVKTLSGNYPSLGYTEDDDR
                     CLYPFALFFISGISFDGDSYYLTGDGGSVDVTVNPDGLYSVKFNDISVGDYELMGDNT
                     TYTEKGKITGSYEGAIHKEVEEVGGTTKGSVARTRLNSLLEQVK"
     gene            complement(23658..24152)
                     /locus_tag="PKK03_04905"
     CDS             complement(23658..24152)
                     /locus_tag="PKK03_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNX83768.1"
                     /translation="MKYLLSILLPLLPLIMSGQAASTEPEVWFTVTRVVDGDTFWVDD
                     GSEKGMKIRLIGIDAPEPRNTGTRPKGFFGAESTSYLRELLRGKKVRLEYDVSRLDRY
                     RRTLAYAYLEDGTFINAELVRNGYATVMTMPPNVKYAETFNELASKARKQKKGLWKES
                     PSLK"
     gene            complement(24192..24659)
                     /locus_tag="PKK03_04910"
     CDS             complement(24192..24659)
                     /locus_tag="PKK03_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83769.1"
                     /translation="MKKMLFIVSLISLSAVAGAQISSNALGARLYGGDSFSGAELSYQ
                     KGLNDRNRLELDASFGFSSEFTRVALVGIYHWDWNIAGGFNWYVGPGASVAYDNFNEN
                     SYVNIGLGGQIGIEYKFKDLPILISLDSRPMWDFLGDVNGLGWGAALGIRFTW"
     gene            complement(24858..25787)
                     /locus_tag="PKK03_04915"
     CDS             complement(24858..25787)
                     /locus_tag="PKK03_04915"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNX83770.1"
                     /translation="MQTILGANGTIGKILAKELPVYTDRIRLVSRNPIRVNETDELMP
                     LDLTQPGAVDKAVQGSEVVYLVVGLEYKLRVWEQYWPTLMRDTINACLKYGAKLVFFD
                     NVYMYDKNHMSQLSENTPVNPPSRKGAVRKWISEMLLEAVKDMGLQGLIARSADFYGP
                     ATENNFVTQMVLKNLSDGKKPMWFVGSDIRHSFTYTPDAGKATALLGNTHDAYGQVWH
                     LPTDSRPVTGKQFISMIATELGTTAPKLTILPKWMLRPLGLFIPVMKEMPEMMYQYDR
                     EYFFDSSKFTGRFGTKATPYEEGIRATCQAYRH"
     gene            complement(25910..>26166)
                     /locus_tag="PKK03_04920"
     CDS             complement(25910..>26166)
                     /locus_tag="PKK03_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83771.1"
                     /translation="QRVSEQRAAEAREAQAREVQARENERRASEAREAQQRAAEQRAA
                     ESRENERRAAEARENERRAAENRAAQQRTLESGSDRISRK"
BASE COUNT         6213 a         6594 c         6549 g         6810 t
ORIGIN      
        1 ctttccgggc tttccaaaga atctgcattg cttcttcttc ttttctggta agtttcttca
       61 tacggcttct gtttttgcaa atatataacg ggaaaaacaa ataaacaact aaattagcag
      121 aaatattact gaaaaagccg taaataagaa tcattgacga atctccacgg ttaacaaatt
      181 gaatatattg taattagcta taggattgaa ttggagcccg gggcctgtct cccttactat
      241 gcctgcaaaa tcggcaccga agatacgcgg gaaccggaac ccggtgacca ccctcgcatc
      301 tcccccctgg tccttatgct ccgcagtggg gcacaggtcc gggccaccta ctccacaaag
      361 gctatgagag cccggaccgt caactgcagg ctattcggca aatagcctgg ttgccggatc
      421 gtgtcttgcg aagagcgcat tgtgtatcat ctcatcaccg tgcaccaggc tgaagcccac
      481 aagccgttcc gtctcaacca tgctaagcgt gcctgccccg gggtgtgcat cctcggtgtg
      541 acggccggca tcgttgaact cgtctatggc ctcaagcgcc ctgaagtaag cttcatgcat
      601 ctccggctgc tccttatcat cgccggggcg cgtaagggtg aaagcggcat cgcaaagcat
      661 cgggaaatag tacctgaagg agtcctcatt tccgaatata tcggccgaga taacctttcc
      721 gtcaaggatg acggccaggc cgttgcaccc ttcggcggga tggcattcag gaaaaacccg
      781 gtctgccttc tgcatcctga cctgcagcag ctcctcatag ctctccgtcc ggctgtgcac
      841 cccctcacat gccaggtttg cgcgtacatg atcccacacc tcgccctgcg tgttgcagaa
      901 gccgggttca ttctcaggct tcatccgcga gaaggtgcct gcttttctgc cccgcagatt
      961 cggatcagcc actgacccgg gtgtgctgaa cgacgatgac cggtagtccc agcgcagccg
     1021 ctccacacac gagacctcta tgacggtctt tgacgacggg gccagcagca ccgacctgtt
     1081 gacaaccctg ttctgcttcg caccggtgag gacgtcggca tcggtgagca ggaggtagtc
     1141 agaggtctgg ttgatggccg cgagctggcc tactgagccg gaggctgtca gctccgagat
     1201 gatgaggctg ccgttcctga tggctggccc ggcggggagg aaggggaatg cagagagagg
     1261 ctggctgctg atctggacga tcacggcatt gccaaaagat gtgaaatgga tcgcttttgt
     1321 tttcatggtt ttgttactca tttataatta ttaattttaa actgtgggca aagaaaaacc
     1381 atggcattgc agcctttctg caatggaaaa aaaagaaaat catgccggca aaccttaacg
     1441 cactgataag atacaagact atcaactcct gccttgcagg gggaagacgc aggtggtcaa
     1501 ttgacgagct gagggcgaga tgctccgagg cccttgcctc agacagggga aggtatgcca
     1561 ccgtctcgga gcggaccctc cgtgatgaca tccgagtgat gcgaagcgac atcctcgggt
     1621 tcaatgcacc gataaagcag gagtcaggat tctatttcta ttccgacccg ggatactcga
     1681 ttctaagtct tcgcatcacc gatgcgggac tggcggagca gatacttggc ctgctgatga
     1741 agatgaggga gaaggtcaac cacccggaga tggaaattat catggagaag ctgagccggc
     1801 tcataggcag ggagtatgga ccggaatccg ggctggttgt cagaaatcag gttaatgatg
     1861 aagcgataaa gattaagaag caggtgatcg aggaagaaat aaatgcgaat gagcgtatgt
     1921 cggctgacct ggagaatcgc ggcatcttcg aaagaagacg gctcagatta atgctgcgga
     1981 agatggacgc cccacccatt gtgcctccgt cggagctgat atggggagaa ataatggctg
     2041 ccgtgatgtc aggaatattc cgtcgtcagc cttagagcgc acagagcctg ttatagacct
     2101 ccgcaagaat catgaagttt ttaaactttt tcaggtccgg gttcatggcc agctcttcct
     2161 gcatcgcggg gcagtcgcca aataactgtt caaacagagg atcaaaattc tccttttcga
     2221 ccttaatcat ctccccgccg tccttcacca cgaaatagct cgggtacctt gccttccctt
     2281 cgatgatatg gtaatcgata cgataggtgg ggccgattct gagcccttcg tccggtgccc
     2341 aagtgaagcc gacgccatcc atcttcgata ttgtctcaat acgtgaggtg gaggtcccga
     2401 tgcctgaccg ggtcacgggg ggaaatcccg ggcagagact ttcatgcccg actgtgccgg
     2461 ataggggcag atcctcaaca cagggaggtg ccggatcgtg tcagagcctc aagacccgag
     2521 gtgccagcag tgggaggtta ccggtcacgg tatgcaaaat gcaacggggc gcatccgtca
     2581 tagttgctgg ttgtggctgc aacgtaagcg tagtcaccga tgacaaacac ggtcttgtat
     2641 gaattgtccc cgcggcatcc gggatagtac ccgcacagga cccatctgtc ataatcagca
     2701 aggtacttgt aaagttcaac gccatcttcc agcacatagg gacttccgct gtaataaaat
     2761 ccaatggcag gaacatagta gtaatctttc tcgaattggt tatccgagac ttttgtccag
     2821 ctgtccgtgc cttcgtcata tgaccatgtg gatccgtatg taaccaaatg tatttttccg
     2881 ttcgcgatgt atgcaaacaa aaacaggttg acggggtgaa ggaagtggta tttctttgtg
     2941 aacccgcgtg tcgaatagtt gtacgtgcag accagtccgc tgtcggtaat tatatattcc
     3001 aggccattca gggtgacgtg ggttgccagt gtaaagatga atggcgcatc gtcctgcctg
     3061 gtccatattt ttgttgtgaa atcgtattcc cagaccgggc atgaggcgcc gtcggtattt
     3121 ccgccgataa cgatgaactt atcatcatgg gggaggaaaa tgttgttttc tgagcgttcg
     3181 cccgggaagt ctgagaccgg atcccaactg tctgcaacag gatcatactc atacagcagt
     3241 ttggcgttta tctcgaggat gtatcccttg ccgttatagc tgaaggcatc ataggtccag
     3301 gtccagtcaa agggagtggg agtctttgtg tcccacgatt ttttaacggt gaaaggagta
     3361 ttcaggccgc cgaatttctg tccgctgaag aaggagacgc taactgcctt atcgtgatag
     3421 aagttgttta caaaaggtat cacggcggtt acctgatctt cttctgactc aattgtttca
     3481 gccacaaatg cgtcaaagtg gagcgtgggc gggccatagt gggtaaggtt tttgcccctg
     3541 atggttatta tgtcaccagc gtaaccttct gtaactgagc tttcaaatat ttcggggcca
     3601 aaaatttcaa atgcattttc tattatgatc tcccggtctc cggaaaccac ttttaaacgg
     3661 acgcggccag ccagaggtgt ttcaggtatt ttaaagacca atgagtcttc agaagatatt
     3721 attatctctg caggggcaat gtcaacgaag actgacaggt tcccctgttt ctggctgaaa
     3781 tttgaaccgt acatcttgac cacggtgccg tcgaatccct ccttcggact gtagtccgtc
     3841 acccttgccg gcctcgaacc cctgcctgtg aacatggtga ctggtccgaa tacagtcgtt
     3901 tctacagtct ttacgaaggg tctcacatga taaacctgat cctttacaat atcagtatgg
     3961 acttctgcct ggaatgtacc gccactcagg atccctgtta attccttttc tgaccaggtt
     4021 tcgaattcag tattgtcagt ctcgttccag acgaatccgt atgaaacgat gttctgagtt
     4081 ccggggctgg taatccgggc ctggaaggtg actccgtctg acagaacatc gacattatcc
     4141 atttgtacga aaggataatc cttcgggccc ctttcagcat cctcacagcc cgcaaggagt
     4201 aaaatgcaga gaaagaggat tcccggttca gccagtttcc ttttcatgat cagaaggaat
     4261 aacttagtaa gacttcagtg aaactcatgt ctgattccct tagtgtcgcg gggaactgac
     4321 tgtatctcac ctccagggag gcagcgttcc tgttgttcag gttatactgc atgcccgccc
     4381 cggcagaata gccaaacctc acacgggaag agacagatga agactcaacc tcattgatga
     4441 ttatactgtt ctccctgatg gtggattcat aaatctctct tttatacttc aaatgccagg
     4501 aaccgatgcc gccggcattg atgaagggtc gccaggtcag tgtcgggacc gtgtaacgga
     4561 gcagcacggg caatcttgcc gagatcaggt tgaccacgac atctatgtct gccatggagg
     4621 taacagaatt ggcgctgaag ccgtgcttag aaagatacac tcccgtattg acggagaaat
     4681 cactcactaa gacgggcagg tcggcgaaaa tccccacctg aggggatgag gccggtgaaa
     4741 aggaaagaaa aggcgcctct ccgacattca gccccaccgg cacagccagg ttcatcactg
     4801 aatagcctgc tgcaactccg aacctgggga acggaagcgg ccggtttatg cccttgttgt
     4861 acatcgaaat cagtttctta agtgaattcc tgtggtaacg ggccagctga acctggctgg
     4921 ctttccagtt aaagtcagaa gtatgttcga ggatcggtgc ccggaaatca tagcgatcag
     4981 gtatttcagc gaagaccgtg ctatccttct ccaggaagta ggttttgagg cctttttcag
     5041 tataatagta aagattaatt ctccccttgc tgattctttc aaggaaaacc ctttcaggct
     5101 gaccgtttac aatgatgctt ctggaaacgt actccggccc gccccggaaa ccatatcctg
     5161 ttaactgata aggaagatac ctgatctccc cggccttcct tttcaccgct acgaattgcg
     5221 agttctgatt tggcaatcct ttaataaggt ctatgccaat cgtcattaca ctatcggcct
     5281 ggatgtaatt attattctgg gaataaactg gttgcgccag tggcaacaat atcagaatga
     5341 tttgcctgat ctttgtgttt ttcatctttg gtctgaattc agaaatctga taagcggtga
     5401 tagtatcaca gtacagtgat ttcaaagtta ttaataacga tttaagaatg ccaggatgtt
     5461 agcctttggc tatagccggg ttgaattatg cctgcttccg gcttattctg ataacgtaat
     5521 tctcaaaggt tttgtacctg aacacccgcc cgtccgtgtc acgttcaaat accagggtct
     5581 cccgcagctc accgtcattc ccgactctcc ggaaggtgtc tttcccgact tgcctgtaaa
     5641 ggaccattga gcctgccggg tcgtcattcg gaagcgtaag taaaataagg tcacctcccc
     5701 atgtggagat atacagttca ctggttgcgg gttgatcaga ataatatccg acataatcct
     5761 ggagatcagc attcttcttt ccgggtatac cggagtctct cggagtggtt gccacagctc
     5821 cggccagtat ggcatttatt gcacctgcat atttgtcggg atttgtgttg ttggcattga
     5881 tcattatgga tattgccagc tttgttttcg gacataactg taaaagtgtc ctgtagcccg
     5941 gacattcccc tccatggcca atccacgtgt tcccatcctc acccctggta acgacaaagc
     6001 ccaggcccca tgttatattc cagtccggat ctgtccagtg gacattatgc atgtatttca
     6061 gtgtggatgg tttcagtact tcttctgagg ttgtatccat caggcggaac tgccaggagg
     6121 caaatttcgc caggtcaagg acagttgatg agaatcctgc cgcaggctta atggcattag
     6181 cctggaaaaa gttcacttct ttcctttcca ggttccggtt cacggcactg tatcctactg
     6241 ccatccttgt cccgtgacag gcttcaggca aactggttcc tgtctcattc agccccagag
     6301 gcccgagtat gtttctttcc atgtattcgt catacggcat acctgatacc tgctcaacta
     6361 cctcgccaag caaactcatg ccaaggttgc tgtactggaa gatggttgat gagggataca
     6421 gcgtctcctg gttacccagc gctgctttta cctggtcacg tgacggaaac cggaagtcgg
     6481 gagccgtcca gtaaggtatg tctgcttccc ttggcaaacc ggatgaatgg gtaagaaggc
     6541 ttctaacggt tattggtccg ctttcagggt atttttgttt aaggtcatac cagggcaaaa
     6601 gatcaccaat ctcgtcatcc agcctgagtt tcccctcgtc atacagtttc ataattgcca
     6661 cagaggtaaa gagtttcgag atggagcaaa tgctgaagat ggttgaaggt tcagccttaa
     6721 ctgaatcttc agcgttagcc agtccgaatg cgcctgccca gagcacctcc tggtcatgaa
     6781 ccaccgctgc ggtcagtgca ggaagacgtt cataatccct ctgtgcatcg agccatactt
     6841 caatcagttt gaatgcttca gaatagtcca tgcgcggact ctgtgacaag ccggtaatac
     6901 tcataaaaac caggattgca atggcatagt atcttttcat tattatggtg ttttgtgttt
     6961 tccccgtcct taattatccg tattatatat gatggcgttg attttattat ttcattgtac
     7021 aatggcggcc agaagccagg taccggtggt gatcacagtc cccggcatgt tgttttcata
     7081 tgactggttc tcaacatagt tccggaacag gccggcatgc tcaggattca gttcaccttc
     7141 ctgaccagag ttccgccggc gctgaagccg gtgactttgc cggccgggtc aagtataaat
     7201 ttcatgtcct cgcgcgactc cctgacgaag aaatccgtat cataggtgaa gtaagccttc
     7261 acccagacag gtcggtggga ttggtccaca gtccggccgc ggcctgctcg ggatagatat
     7321 ggaatttgcc atctaccctg gtgccgtctg ccttgtaacc tgttgccagc aggctggtgt
     7381 ctgttccggc cggaggttgc ctgtatgaac tggatttcat tcccataggg tcaatgacgt
     7441 tcttctgcat atactcatca tagggccggc ctgtgacatc ggtcactatc tgctgggtta
     7501 tcgtggttcc tcctcctgaa taaacaacgc gggtaccagg ctccatgaag gagcgtacag
     7561 cttcagtgtt ggcgggcttc tgtccgtcga ctacctgctg aaccgacggg agagtgtcgc
     7621 cctgtgcgta tccgggaaat ccgtgtattg tcagccctgc agtgtggctc aggagggcgg
     7681 acagtgttat gggcttgccc ccggagacac tgtcataggg gaatttccat gatttaaggt
     7741 actgatttat atcagtataa agatccagct tcccgtcctg caccagtttc aacacaccca
     7801 ctccgttgag cgacttgctg atagatgctg cctggaacag ggttttctcc gtcaccggtc
     7861 gcttttcgtc gacatcggcc cagccgtaac cttttaccca gtcgcccagg ttgttttcga
     7921 ccagttttat cctctcctcc gtttcgggtg aataccggga ttgtgtccct ggctggcagc
     7981 cggtgatgag gagaagtggc aacaggacgg caggcaggca gataagatta cgtttcatgg
     8041 cagacatttt ttggggttat tcatggcaga tcatcggatc tccgtcatgt aaaaacaaat
     8101 atatacagtg ttaatcacct gacaaactgt tcagctatga agagagcgat tagtgcagga
     8161 tgcttttata atggcctaag ccgtaccggc ttactttctc catgtattta tcgaaggttt
     8221 ccccgaatgt ctccctgcac caccgctcct cggcgaggat catgaagtga acggaaacct
     8281 ggaaaaccag caggattgcc agcagaagcc acgaacgggt aaccatgcat gcacccagaa
     8341 agtagaggaa aaatgcgacg tacatcgggt tgcgcgagac ggcgtacaat cccgaccggt
     8401 ttatgccgcc ggcatcaggc ctcgcgaatt gaacgatgct cataccataa agaaccaggg
     8461 ccgccagtga caggcagacg cctgcaatac ccatgaaacc gttaagctta atcctcagga
     8521 aaaaaggaat gaccagaagc agcagggtgg tcccgatatt taccaggtat gcaggtttct
     8581 caagaccccg ggtgggaggg aagaatgccg cccgcctgac agcctcctta ctcagaagcg
     8641 atgtcagcag atacctggtc agcaggatgg gaatgacaat aatcaatccg gtaatgacag
     8701 tgggttcaac tatcattttt ctaacctgct taaggcttcc tcagccgttt ccacgaagtt
     8761 tatcgttccg cgggtgttgc tctcgcggat gaaatcgcga agtgccttgc tcctgacatt
     8821 cgcgaagtcg ccgatgatgg caagtctcat cctgtagttg gagaactttt gcagtatctc
     8881 acccgcgatg cctgtcttca ggtcaaagaa actgccggta agtgttgcgg aatggattat
     8941 cagcccggtg ctgccgttat agccggcatc ggcgaggata tcaagcaggt cttcagtggt
     9001 gcctattgtc tctgaaccag gcaggatctc ggcaatggat atgccttttg ctgtcttgtg
     9061 gtacttaatc attgtaaaga aaaaataagg gtttatctcc agatgtaaac ggttccttca
     9121 agcggtagtt tttccgggtc agcctggtgc ccgtactcat cccaccactc ctggtaaagc
     9181 tttcttacgg ccatgatctc agcggcgtcg aggcgctgat cattggggta atccttattg
     9241 tagagcaccg ggttgagcga ggcgaagtcc tttccttcaa tgacagcctc ggcacaccat
     9301 aacaggtatt caccaagcat aatgcattcc ctgccgtcaa caaccctgaa cggccacatg
     9361 gaagatatcg gattgaccgg gaaatgatcg cacggggaga tcagtccggt ttccttcgcc
     9421 aggctgatca gttcaggtat atctttcttc tgaaaagcag gcatcttcgt ccagagccgc
     9481 tcaccatctt cggtgtattc atactggtta taggtcccgt tttcaagttg tctgacgaac
     9541 ccggtgacat caggattatt gaagtcgaag ctgcccttgt cgcatccggg gcacagaagg
     9601 gcaataagtg ttaatatgac aatagatttc acaatggtac ttttcacatt gatgcaatta
     9661 tcctttcaat ggttgcgttc ttccggcctg tcatcctgca gcgcttcggc ctggttctcc
     9721 acggatatgg ctggttcatc cgtcctgcca aagagcttcc ggcggatagc catgatgatg
     9781 gctcctgtct tttcaatctc gcgaagcagg taatctcttt gttccatccg ggattgtgat
     9841 catcagagac cagtcaggct taccgtcaca ggctgagtca tcaggttcgt cctgtaggtc
     9901 agactgtcag acccgttcag aatgagctct tccataacaa cagaggtgcc ggagcttgaa
     9961 aaggtcttct tcccctcgtg catcttccac cagtcgggat tgaggcttat cttcatcgta
    10021 ttgaatgtct gaacatccgt aacgaacagg tgccagctgg ttacgaagat ggctttattg
    10081 ccgtcttcag tccagtaaat tttatgcgcc gactcaatat cctcgggcag gctggcaacg
    10141 agcctgaact caccggtgcc ggcttttgca atgaaaagac tctgtttggg cgggtccgcc
    10201 gaaggacttt caaccacata tgcttcaaat gatccctgcg gggaattgac ggacatgatt
    10261 ttgcccggtt tggaactgaa gccgaagata agataaactg ccacagcgat caggattatc
    10321 gtaaccagcg ctgtgatcat tactgtcaga attctgcctt tcatggttct atgtttgagg
    10381 ttactttccg tgcctggcct ggaggaatcc gatggttgtc ttcatcagct tcttcagagc
    10441 ttcctggtca atgtcagaca gctttttaac atagatgcac gctgcaccct tcttgaattt
    10501 gccgagcccc tccagcaggt gctcatgttc gggcgccccg gtataaacgt acagcgagat
    10561 agccgctttt cgcggtgaaa aaccaacgag tggccagtcg ccctcctggc tgctgcgctc
    10621 tgatttgtaa tgatactgtc cgaatccgat catggatgca ccccacatct tcggctcgaa
    10681 gccggtgaac tcacgcatca gcttcaacag ttcgaaacta tccttccgct tttgttcggt
    10741 gtcggcaaat gagttaatga attcattaac atcagacccg cttttctttg ttttaagctc
    10801 tgccattgta gataactttt tctgataaag gaaattacat tcaaggagtt cacagatcaa
    10861 tccgcagatg taaaatccgg acaacaattt acgaaaatca aagggaatcc cccggatatt
    10921 acagcctgta tctcttccgg aaggtactga ttgctttcat gaccgcggca ttgtcgccga
    10981 agaagggcat atgtgccgca tcgatgctga ccagcatcat ctcagggaac tgtgccgttt
    11041 tgtagtgctc cggtccgatc atccaatccc tggtactgta tatgaagaga acgggcatgt
    11101 tgaccttcgc ggtggcttca gtgaagtctt caccataatc atccatcctc atcgccgctt
    11161 cgctgaaatc gccgttccac gatggcaact caccgtatgt gctgttcatt atattcatct
    11221 cttcgcgcga agcgaaaccc atcttccaca tcatgtcact gctgttcagg gcagtgatca
    11281 ggctgtccca acggacgtgg agcgggatcg attcatccac gaaatagctt ctgtcagtga
    11341 ttcccagcaa ttcacaggct ttcgggcacc agctggtctt gtagttcagc cccatgttca
    11401 gcgagcagtt tatcattacc atggcttcaa ctgccggcgg gtgcctcagt gaatagccca
    11461 tctgcagaag gcctccgaac gagtgcccca gcgtgatcca ccggctgatt cccagcgctt
    11521 cccttacctc ctcgaagtct ttcaccattc tgtccatcga atagttgccg tcagcgggac
    11581 tggaggagcg gcccacacct ctctggtcga gataaatcat ccggaaatgc ttttcgagcg
    11641 agtcgccggc aaacttctca agccagtatg acccgcttcc cgggccaccg tggagataaa
    11701 gacagggagt tccttcacct ttgacattta cataaaggtc tacaccgtca gtagtaatga
    11761 tgtgcctctc ctgggcacat accaccactc ccagcaggag ggccgaaaga aatagtaatg
    11821 actttttcat atcaatatgt tttgtgtcgc ttctgacctc aggctgatgg ctggggatga
    11881 ttcccaggct aaagaccttc cggattcaaa agcgctgcaa aatggcagac aaaaaggtct
    11941 tccgattcgt ccacctgaaa ttgctgttcg tcgaccaaac cttatcaaat gtcatcaggt
    12001 aatataaaag gatatccggg tcctttaata ttgcatcata aaaccattaa aaacaaaaca
    12061 gtcatgaaaa aacactttaa actgctgggg gccgctgtca taataggagt agcacttggc
    12121 tcctgcgaaa cggatgaacc gattgacgaa tctgaatttg gtgatcttaa gattgcctac
    12181 aatggactga taaagcctac tgacaacagt attgtgctga aatggaatga ggctgtaagc
    12241 aaggtggtgg acaacaagat gccgcctccg ccggaggcga ggatatatgc catggtgatg
    12301 cttgccatgc atgatgcgct taaccaggtt atcccttatt atgagaccta tgcgcttgat
    12361 aacagcggtg taaacacgaa gggcattacc aggaaggtta tccagggcct tgccaatgct
    12421 gcagcgtcac aggctgcaca tgatgtcctg gtggcactgg ttccggctac ggagcgtgat
    12481 gcaggtatac ttcttgaggc ctgtctggaa gaggttgaag catcagagta cagggacatg
    12541 gccattgatg caggtgcaag ggcagctgcg gctatgctcc ttaagaggag tggagacctt
    12601 ccgctcattt tccaggctta tccacaggga actgagcccg gtcagtacag atcgcccggc
    12661 aattttgcaa atccaggccc gaactggccg gccaatgcgg cttatgcccc ggacctcggg
    12721 gagcttgaac cgtttggggt cctgtcaagt gaccagttcc gtgcaatacc gccgtattct
    12781 gtcggttctt tcgaatacgc cgatgagtac aatgaggtga agctcctcgg cagcaacagc
    12841 agcaccgcaa gaactccgga gcaggctgag atgggattat tcttccttga caacgtggca
    12901 agctccatca acagggttgt acgggtagtg gcagtcaaat tcaatctcga ctgctgggaa
    12961 acggccaggc tgttggctct tgtccatatg gcccagttcg acgcagttca gagttcattt
    13021 gaagggaagt atcattacaa tctctggagg cctgaaactg ctataaggct aggcgaactt
    13081 gatggcaatg gagcaacgac gggtgatgcc gggtggtcta ttctgagtgg cgcaaggaat
    13141 acgcctccga ctccgacata tccttcaact catgcagagg ccgcaggggc agtaactgag
    13201 attctgcatc tctatttcaa gagggataac gttcagtaca agataggcag ttactcgctt
    13261 ccgggtgtgg agcgcagttt ctcctccttc tcgcagttcg caagtgaatg cgcagaatca
    13321 aggatctaca tcgggtacca tttcaggaat gatgtcatac atggagaaat gaagggacgt
    13381 gagctggcaa ggttcgtctt tgccaataac ctcagggctc tttaaggttg cagttttcca
    13441 tgtaatgtga aatccggccg gacactttcc cggccggatt tttcaatcat tgactaaagc
    13501 tcaatccccc tgaaaacggc attaccggat gtaagtttct catgaacttc atggtaactc
    13561 cttccgatgg gcaattccct ggtgcctatt tcgacatcat ttgttgtaaa tgctgtcacc
    13621 ttcggcactg acacgatgaa ggagcggtgt atccggagaa accggttatg gggcagcatg
    13681 ctttcaacac tgcttattcc gcattttact ttcaggtcag ctgcggtttg cctgtggatg
    13741 atgatataat ccctgaagct ttcgatatag gtaatgtcat cgagaaagac cttcaccatc
    13801 ttcctttcag tgcggaagta aaggaagccc ggagagtggt cgttcaactc cgggtcaggg
    13861 gtgcatacct gccggaattt gtttacagct ttcatgaacc gctcgaagga gacaggcttc
    13921 atcaggtagt caacagcatc aagttcaaat gcctctgcag catagtccct gtgagcagtg
    13981 atgaaaatca cccgcggggt agtccggatg gtcctgacca tctgcgtacc cagcatctta
    14041 gggatactga tgtcgagaaa catcaggtca accttcatcg agttcagcat ctctattgcc
    14101 tcaaaggcat tactgcatgt tcctgcaacc tcgaggtaat cgagggattc gatatagttg
    14161 cggaggactc tcgccgccgg gggctcatca tcaacaataa gacatttcca tttctgcata
    14221 tctttcctcg gttttttcca gtgtcataac aagatccacg acaaaagtct ctttcagcct
    14281 ggtcgtcagt aaacggtgcc tgtcagcgta catcagttcc agtcttcttc tgacgttcgc
    14341 aagacccaat cctcctgtat ttgcaggttc ctcatccttt tcaggatctg cgctgttgac
    14401 cagtttaaaa tacatgaccg ggcctgccac ttcaatatcg atggttatcc agcaccgtcc
    14461 cagttgcttt ccggtgccgt gcttaaatgc attctcaagg aaggggagaa gcaggaacgg
    14521 ggcaatctga tatctgccgg cctctcctgt tactgtgact gaaatttcaa ggcggtctcc
    14581 atagcgaatc tgttcaaggg taatgtagtt tcgcagcagt cctatttcct ttgcaagggg
    14641 aattttaggg gcattgctgt catagatcat agacctcagc aattctgaaa gtttcagcac
    14701 tatttccgga gatctgggtg atgcctcgag ggtgtgggag tagaggttgt tcagtgtatt
    14761 gaagagaaaa tgaggatgga gctgtgcctt cagcaactgc agttccactg ctgttttctt
    14821 ctgttccgct tccagggttt gctcacgctg ttcataccag tacataagca tcctgactga
    14881 aagagctatt ccagctacat gaagataggg gagcacattc ctgccgactt taagggtcat
    14941 gcccttgaga ggggctgaag catcaaggcc cagctgggcc aggttggaat agatcataca
    15001 gattgccagt acaatcccca gtccggcaaa aaacgaaaaa agcttctttt tcaaaaggaa
    15061 tcttggtacc agcacataca ggagcgtgta taccatcaga aagaatccgg gaagataaag
    15121 tgcggaattt atcagagacc ttccgggatt tacattcagg ccaaaacccc aggcaaagta
    15181 tataaccgtc ataatccaga acagaatatg caggagtacg gttctgacaa tagagggaaa
    15241 cactatccgc ctgaatttca taaccggttt gttttcagta ataaggatta accggctgaa
    15301 aagttcacgg aaaatattga gaatgagcag ttattcaatc agcaaagagc cgccagatgc
    15361 tgcccgccgg tggtttctaa aaaaagagag cctgcgctgt ggcaggctca aatctcactg
    15421 acaccccgga ttttatcata tcggcttcac cggcacgcag atgtcaacca cgaatttccc
    15481 gtcccttggt tcctcggggt acatctcgaa gcagggcttt tcgtccggct ggtaaccgct
    15541 ggcaggaaac cactctccgt atagccagtc ccatgcctcc tgaaactgct gtgcattgac
    15601 ctcgaagcgg gcaatgacat attttgcagc ttccagctcc atctttccga tctccccgtc
    15661 aaccttcgtt tccggcggta ctgtgataca gacgctcatc cttagcttgt cctcaattgt
    15721 aacaccggga tcatcatgat agatcaccag tgacctgaag cctttgccgc caatgaggcc
    15781 gcgcggaccg gcccatgcaa acaggctgtt ccacagaccc tcgaaaagct tgtcgtcacc
    15841 cttgtacggt ccgatgtgcc tgatgtaggc aagtgtcatc ttcggcagtt ccttaacctc
    15901 aacacttttg ttcagtttca tatttgtcct ccatttaatt gtttgtaact caggacaaaa
    15961 ataagggacg gcttttgctt cggcttgact gcccttgcta accgtttgac ttaaattgct
    16021 attctgtggt ttgttcgccc tgtaacttgt aggggatacc ccgaagcagc ttttgaaatt
    16081 gcgtgaaaat accgacagat ctgagaatcc gcatttcgta gctatcccgg atacggtcat
    16141 cttcctgttt gtcagaagaa gcatggctgc tttttctatc tttacccgct ggatgaactg
    16201 gaaaggggtt tcgcccacaa tggagtggaa tatcctgttg aagtggtatt tggagaagtt
    16261 tgccactgat gccagctcac ccagggtcat cagattttca atgttggagt ctatataatc
    16321 cagggttttg ttgatccttg agatatactc tgatctggag gttttcggtt cgtccattct
    16381 gacttgaatg taacagcact tttacttcat gaagttatat ttcacctcga cggtcacatt
    16441 gccccggagc atcgaccata cagggcagta ggtcgcctcc gcaagcttca ccaccttgtc
    16501 cacttcggct tcatgaagat tatcggatgt gatgtccatt tccataatga tattagagaa
    16561 gcctgtcgga tgctccggct tcctgaaacc tttcgacctg atttcaaagc cggtaatggt
    16621 tttgcccatc ctcctgagga aggtgagggt tgcacttccg aggcacgatg agaggcttag
    16681 taacagcaac tccagtgatg tgtatcccag gttgtcaccc aggggcgggg tgtaatcaat
    16741 tgataccgga atgttcccct caacagtgcc ggaaaagttc agtttgtcat tgatgagcct
    16801 gacagtggct gtcagttctt ttgatttgtc cattgcaagt atagagttag gattaatgtg
    16861 caggtccggc ttacagccag gggagcagta cctttttgcg atagctgtcc catctttccc
    16921 cgaaaatctt cgcgaggatc ttctcttcct caggagcata gagccttgac cctgtgtaga
    16981 ccaccagccc tatcagcagc cccagccagg tgttcagcag gaacccgaac catggaagca
    17041 ccagcagcgc cacgcctata tatagcgggt gcaggaccag ggcataaggc ccggtggtga
    17101 taagctcatg ccgtggtact ttaacaagta tcagtacaac tgaccagatc cagattatta
    17161 ctcccggtac gagtatggcg gcggatagcc atttgagggt atccgtcgga ccgccaaccc
    17221 tgaaaaactg cgggaaaagt agattgaggg cgactcctgc caccaggaag ggaagggtga
    17281 ataaccctat tttccttccg ctgccgacca gaattttcag tttcatgata tcatttttaa
    17341 tgaattgttc tgtctgctgc ccgcacctca ctcatgaacc tgccaagctt ttctgcgtca
    17401 agcagaccgt tggtccttac cccgctgcat acgtcgaccg caaacggctc aacagtctca
    17461 atggcctccc ttacattctc cggcttcagt ccgccggcaa gaaacaccgg gcaggaggcc
    17521 tgctccctta ttctgcggct cagttgccag ttatgcaccc gccctgttcc tccgagttcc
    17581 ttcaccctga gattcgggtt accgctgtca agcagtattg catcaacaga ccgtgagatt
    17641 cttacggctt cctccacaga ctcctctctg agcacatgaa ccacctgcac aatcttaaca
    17701 cccggcaggg cagacttaag ctctgcgtag gtaccctcag acagtgcatc aactatctgg
    17761 atagtattcg tatgtgtcag acggtggtgt tcaatgatct ccctgaccga agtccggctt
    17821 gtgagcagga atgttgcaat tggcggtggc gttgtggcgg cgatctgcct gatgagctca
    17881 tcaggtatgg gaccggggcc gctgggcatg gctgccacca ggcctattgc agaagcgccg
    17941 tacctcatgg ctgtctctgc ctcacggata tcatggatac agcatatctt tattcttgtc
    18001 tccatctcgt cgctgtaaat tacggcttta attgcctgag cattgtcatc gattcatcat
    18061 gacgggcata atcttcaaac ccttgtttcc tgtacatccg gtatgcatag ttggcaaggt
    18121 caacgctcag ggagacctgc ctgaagcctt ttgaggcaag ctttttgagc attccggcca
    18181 gcagcatggt cccgatgccc tggcgccggt attgctccag caccgacatg ctcagctccg
    18241 gaatctccgg tgctatgaat ccgtaaccct gagctccggc agtgaagagc ctggtccata
    18301 ccgcaccggc aatgacacct tcaatctcag ctacgaggca aatatcgccc tctcttccga
    18361 aatctttaat atatacggca atttcaggca gttctattat ctccggaggg ggttttacct
    18421 cttcaggagg tatgaatatg gcattgtaga gcatcgtttt gaggatgcct gtttctgatt
    18481 ggtcaaggtc tcttataatg atttctgcct ccatttacgg cattggctac cgggggttta
    18541 gttctccggt ctccacggag ggttaaaggt aaaaaaaatg tcagttgccg ttcaggccca
    18601 cagcaagctt ttctgcaagt tcctcaatat cctttcttcg tgcaatctgc tttacccatg
    18661 aatccggtat ggtgtcaaat ccgtaaagca gcccggcaag cccgccggtg acggctgctg
    18721 tggtgtcggt gtcactgcca aggttgactg cgcgcagaac tgcttcgcta tagctgcctg
    18781 tggtgagaag gcaccaaacg gctgcctcca gggtatcaat gacataaccg ccgctgcgga
    18841 tttcgttttc attcagcctg aatatatcat acttgaggag gcggtcaaaa accaccacct
    18901 ctgccgggtc aatcagcagg gctgagaggt ggccggaaac gattttctgg agatccctgt
    18961 atatatcata cttctcccgg ccttcaagga tctgcctcgc aaactcgagg tagtagaagc
    19021 aggatattat tgaccgtatg tgtccgtggg tgacggaaga gacctgacgg gttatctcga
    19081 aacgctctgt cagcggcctt gggagcagcc ggaacatcag cggcagtatc cgcataagcg
    19141 aaccgttgcc gtttgatgac actgctgtgt cacccgacag ctccggattt gttcctttga
    19201 aaatacgcac aatagcctca ccggtggatg tgccaatgtc aaagaccttc ccgccggctg
    19261 tccagtaatc cctgaagtac cagttcacaa agctctggcc aattgcactg aggtcatatc
    19321 cctgagtcag cgcttccgcc aggcagaagg tgagggagct gtcatcagac catgtgccgg
    19381 gaggctggtt atgaaccccg aaacctgtca tcccggttac cgggtggaga ttcatgtaat
    19441 ccctggattt aaattcgaca ggcactccca gcgcgtcgcc gacggctacc ccgaaaagtg
    19501 ctgattttat tgcatatgaa tagtcatcgc catgattggc gtggtccggc tgcttcgctc
    19561 cctggctcat cttgtccctt taaatgttct cccccctttc gggttcatcc cttgactatc
    19621 atttaacggc aacgggaggg tgaatagtat gactgtgact tcatcagcag tcagtgattt
    19681 atttcaaaag ggacagattc tgagtttttc ataaattgta ccattaacca gaaaaacact
    19741 tgctatgaaa atcttaagag gtactttatt cgggggaata gcttacttcc tcattggctg
    19801 gctcgtttac ggcgtgctgc tgtacaattt cttcgctccg atgaccaaca catgtgccaa
    19861 tcgcccggaa ggggagatga tctggtgggc aatgatcctg tccaatctcc ttatcgcatt
    19921 gtttttgaca ctgttcctga agtggtccgg ggcccgcggt attgttgacg gactgaaaac
    19981 aggcgcgctg ttcggagctc tttttactgc caccatcgac ctgtcgcttt tttcgatgac
    20041 cactatgtac aactcgctgg tgcctgttgt gctcgagacc cttgccagcg ctgtggtaat
    20101 ggcaattgtg ggaatgatca tagtacttac gtggggcagg ccgaaggaga tatagtacag
    20161 tataatgaag gcggttgtat ataacaggga agcaaagccg tacaggcttg ttcttcgaga
    20221 ggttgagaag ccttttccga aggatgatga ggtgctggtc agtatacgcg cggcctcggt
    20281 caatgctgca gattaccgct cgatgaagat ggggatgata cctgcccgta agattttcgg
    20341 ggcagatatt tcgggagtag tggaatcagc cggcaaaaat gtaaagctgt tcaggccggg
    20401 cgatgaggtt gtgggtgatc tctcagggtg cggattcgga ggttttgccg atttcgccct
    20461 ggctactgag aaagctcttg ttcataaacc tgcaggcctt tcatttgaga tggcagcggc
    20521 cctgcccatg gcctcgatga ccgccctgca ggctctcagg aacaagggac agatacagaa
    20581 agggcaggag gtctgcatac tgggtagcag cggcggggtg gggacaatcg ccattcagct
    20641 ggccagacat ttcggcgcgg taataactgc cgtatgcagc acgcagaatg tggagcaatc
    20701 gcaattgctg ggcgcagacc gtgtaataga ctattcgaag gaggattttg cggtgggaaa
    20761 caggaaatat gacctgatta tagctgttaa cgggaattat tccctgctga aatgccggaa
    20821 ggcactgaag gccgatggaa ggtatgtgct ggtcggagga gctctttccc agatctttaa
    20881 ggccatagtg atcgggaagc ccttatccct gggcaggaag aagatgcttt tcctggctgc
    20941 aaaaacggac accaacgacc tcaggtacct gctcgggctt gcatccgatg ggataatcaa
    21001 accggtgatt gagaaatact tttcaccgga aacaactgct gaagcaatga agtatgctgc
    21061 cggcggacac gcccggggca aggttgtgat aaagtggaat cctgaggctt aagcaaatgc
    21121 ttgactcaat gatcagttct ctctccaaac aggagcaaca cggaaggtaa gggatctcgt
    21181 ataaccggcc caggatgcgg cacgggacat gcccgtggag tgagtttcac tcgaagattc
    21241 tcgtaccgaa gaggtaccga tccttccagt acggatcatc cagcggagtg attgtcactc
    21301 cctggctgga agaggtatgg atgaacctgc cctcaccgat gaaaatgccc gaatgagtta
    21361 tcagccggct ggtgttgtat gtcctgacga aaaagaccag atcgcccggc atgagcagct
    21421 ccctttcagt aataatcctg ccgtacctgg cctgttcctc tgagttgtgc ggaagtatta
    21481 tcccgtgtgt tgcaaaaacc ctgaatatca gccctgaaca gtccgtgcac ttctccgtcg
    21541 tgccacccat gcagtgcggc acacccaggt attctcgtgc cgtaccgatt atcgcagcgg
    21601 gggagacagt accggtacca actgaacgtt caatcccggc attcatgaac tgttccagcc
    21661 gcttcgtttc ggacggactg atggagacat cgggtatacg cagggaaaat tcaggagttt
    21721 tgatgagaac accgcctgca cttcgcccgg agttgccatt gcctgagtca gcaggcacag
    21781 ccctgcgcga acaggatgca gccaacagca gcattgcaac tgccagaaag acaaatatca
    21841 atcgtaattc cttcaattgt attccggatc ttatgtcagc ctctgaagcc attttcccgg
    21901 gtatcagagg agctcgatgg tgatcgtgtc atttctcctg gtcctttgcc cggcaacgtc
    21961 aatggtcatg ttcctcttaa attcaatatt gacaggaacg ccgggataat agtaatagtc
    22021 gtaccggttg ctgatattga tctcataaac agccatcgcc agagcctcgg tcagtgatgc
    22081 gtcttccacc cccatcctgt caaaaatact ccggaggaaa gccatcgtat catccctgtt
    22141 cagtttcgac tcctgcacca gtacgcttcg ttcttcttcg aaatcgacct cttcgaacca
    22201 cagttttgca tcagccctta tcggatcgcc gccgagaagg tttgggaaca ggtcttcgta
    22261 tgaaatggtg tcagccacgg gtatcatcgc cccgaagggg aaatgaaaca gcatcagctc
    22321 cgggaaggcc atctgttcca cgccctcctt tgaactgtac atgtcgatga ttggccgggc
    22381 agtctgcatc agcttttcat attcctctcc ggagtctttc gagatcaccc tgaagacgtc
    22441 ggaaaacatg gaacgcatca tttccccgag ctccttccag ttctcaatac cgttgaactc
    22501 gccaagttcg gttgtggtat aaactacttc agttactgag tatttcgaga gctcttcaag
    22561 cagttcgtca ggtatatccc acgtattgat aagctcattg ctgaatgtcc agaccacggt
    22621 gtaggaggtg tcagttgaat caaccacctc gaagttggct gtatactccg aaatgtcatt
    22681 ttttgtgagg gagtcaccaa cccactgcat gctggttttg tgaaccctga aatcgtatga
    22741 atcacctgtg gaccagtatg ctacataaga tactgaggtt gtgtcaacct ggcagaacag
    22801 gcctgttgca acggctgtca gtgccagcag aagggcaatt tttttcatat gacgggtatc
    22861 agacggataa tgtattctgc tgtaaaacta tcaattttta tttgctttcc tgcaataatt
    22921 cagcgtttgc ctgtgtttca agtaatacgg gtctgcaacc gtataacggg aattgtcttc
    22981 tgataactca tttataatga ttcattaaac caattgtcat gaaaaaaaat gcaattctgc
    23041 ttgtcgggct gtttgccctg ctgacactgt tttcctgttc aaagaacaat gatccggtga
    23101 actcatttac atttgacgga gatgagtacc tgatcaatga tctttacctt atagaagagg
    23161 tcttcaataa gggagcttca aatgagatgc atgtcttcca gttcatgttc ggaaacattg
    23221 aaaacggaga cacaaccatg cttgcgctgg ctgttctgga taagaatgtc aagaccctca
    23281 gcggcaacta tccttcactt ggctatactg aggatgatga caggtgtctc tatccgtttg
    23341 cactgttttt catcagcggt atttcgtttg acggagattc atattacctg accggtgatg
    23401 gcggaagcgt tgatgtaacc gttaaccctg acggcctcta ctcggttaag ttcaatgata
    23461 tcagcgttgg cgactatgaa ttgatggggg ataataccac atataccgag aaagggaaaa
    23521 tcaccgggtc atacgaaggt gccattcaca aggaagtgga agaagtggga ggcacaacaa
    23581 agggttcagt agcaaggacg cgtcttaact cgctcctgga gcaggtaaag taaccgtaca
    23641 gtcagcttcc tgttatctta ttttaaagaa ggcgactctt tccagagtcc cttcttttgt
    23701 tttcgggcct tcgaggccag ctcattgaat gtctcagcgt atttcacatt gggaggcatt
    23761 gtcatcaccg tggcatagcc gtttcgcacc agctcggcgt tgataaaagt gccgtcctca
    23821 aggtatgcat acgcaagtgt gcgcctgtaa cgatcaaggc gtgagacatc gtattcgagc
    23881 ctgaccttct ttcctcttaa cagctcccgg aggtaactgg ttgactcggc accgaaaaac
    23941 cctttcggtc ttgttccggt gtttcgcggc tcaggcgcat caatgccaat aagccttatt
    24001 ttcattccct tctcgctgcc gtcatcaacc cagaaggtgt caccgtcaac cacgcgggtg
    24061 acggtgaacc acacttccgg ttccgttgat gctgcctggc ctgacatgat caatggcagg
    24121 agtggcagca gtatgctgag aaggtatttc actgtctttt aaatgcatcc gggtttaccc
    24181 ggtgtcctgg gttaccaggt aaaccttatg ccgagggctg caccccatcc cagtccgttg
    24241 acatcaccca ggaaatccca catcgggcgg ctgtcaaggc tgatcaaaat aggcagatcc
    24301 ttgaatttat attcaatgcc aatctggccg ccaaggccga tattgacgta ggaattttca
    24361 ttgaagttgt catatgcaac acttgcgcca ggacctacat accagttgaa tcctccggcg
    24421 atattccagt cccagtgata gatcccaaca agcgccaccc tggtaaactc actgctgaag
    24481 ccaaagctgg catcaagctc aagcctgttc ctgtcgttga gtcccttctg gtatgacagt
    24541 tctgccccgc tgaagctgtc gcctccgtag agacgggcac caagggcatt ggaactgatc
    24601 tgtgcgcctg caacagcgct aaggcttatc agtgaaacaa taaataacat tttcttcatg
    24661 ataaaaaagt tttaatgttt tcctactcgt ttggcttttc ggttccgcct tacttttttc
    24721 agtattatta acagcctcta tcggcaaaaa tagcatgcct gtctaaaaga atggcattac
    24781 cggttaaaca aaattcattc caaaatggga ggtgggtgat gcctgatatg gccactcaga
    24841 atgcattgca ctgtttatca gtgcctgtaa gcctgacagg ttgccctgat tccttcctcg
    24901 tagggagtgg cctttgtacc gaaccggccg gtgaatttag atgagtcgaa gaagtattcc
    24961 ctgtcgtact ggtacatcat ctccggcatc tctttcatca cggggatgaa caggcccagc
    25021 gggcggagca tccatttggg aagtatggtg agttttggtg ctgtggtacc caattcggtg
    25081 gcaatcattg atatgaactg tttgcctgtg acaggcctgc tgtcagtagg aaggtgccag
    25141 acctggccgt aggcgtcgtg cgtattgcca agcagggctg tcgccttgcc tgcgtcaggg
    25201 gtgtaggtga aggagtgcct gatgtcagat cccacaaacc acatcggctt cttaccgtca
    25261 gaaaggttct tcagaaccat ctgggtcacg aaattgtttt cagtggcagg accgtaaaag
    25321 tcggccgaac gggctatcag cccctgcaga cccatatcct ttaccgcttc cagaagcatt
    25381 tcggaaatcc attttctgac tgcacccttg cggctgggcg ggttaaccgg ggtgttctcg
    25441 gaaagctgtg acatgtggtt cttatcgtac atatagacat tgtcaaagaa caccagtttt
    25501 gccccatatt tgagacatgc attgattgta tctctcatca gggtcggcca gtattgttcc
    25561 cataccctca acttgtattc aaggcccact acaaggtata cgacctcgga gccttgcacg
    25621 gctttatcga cagctcccgg ttgcgtcaga tcaaggggca tcagttcatc agtttcgttc
    25681 accctgatcg ggttccggct tacaagccgt atcctgtcag tgtacacggg aagttctttt
    25741 gcaaggatct ttcctatggt accgttagcg ccgagaattg tctgcattgt tgttttttta
    25801 acgaatatag aacaattata atgttgatat gttccggcaa gtccgggata gaataataaa
    25861 gtcccgctga tctggtggtc agcgggacta ttgaacagtc agttaagttt tatttgcggc
    25921 tgattctgtc ggaacctgat tccagtgttc tctgctgggc ggctctgttc tcagcggctc
    25981 tcctttcgtt ttcacgggct tcggcggctc ttctttcatt ctcgcgtgat tctgcagctc
    26041 tctgctctgc ggcacgctgc tgtgcttccc tggcttcaga tgcccttctc tcattctcac
    26101 gggcctgtac ctcgcgggcc tgagcttcgc gggcctctgc ggcacgctgc tcgctcaccc
    26161 tctgct
//
LOCUS       DAOPXU010000032        25518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_1924_length_25518_cov_12.365079, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000032 DAOPXU010000000
VERSION     DAOPXU010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25518
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1924_length_25518_cov_12.365079"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PKK03_04925"
     CDS             complement(<1..401)
                     /locus_tag="PKK03_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNX83772.1"
                     /translation="MKHRIESAWKGKMKFDTEVNGHHFFIDQAGENGDNSGPRPKPLM
                     LAALAGCTGMDVISILKKMRQEVEYFNVVVEGDTEDEHPNAYVRMHIIYEFSGRDLEM
                     EKIEKAISLSMERYCGVSAVYRKAGIEMTHEV"
     gene            613..1308
                     /locus_tag="PKK03_04930"
     CDS             613..1308
                     /locus_tag="PKK03_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNX83773.1"
                     /translation="MKTITNTDREFVCDITLPCFSALTPEETELVRESRVQVVFRRGE
                     NLAKQGAFASYVIFTVTGYSRQYIECSNGRDYNLRIIRQGEMVGLSAVFNRNTFSYSV
                     VAVTETTGFLIEKDAIAALVKQNGTFAFSIIRRYCEHNSLLYTSLDNVLHQQTNGRMA
                     TVLLSLAPENFGGEDLFRHLSRRELADFAGISTEGAVKVLKSFEKDGLIKLSDKSITL
                     TNRPRIEEISRTG"
     gene            complement(1428..4007)
                     /locus_tag="PKK03_04935"
     CDS             complement(1428..4007)
                     /locus_tag="PKK03_04935"
                     /inference="COORDINATES: protein motif:HMM:NF037222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glycosidase"
                     /protein_id="HNX83774.1"
                     /translation="MSLTFLASSQETPITTGWRAIRASELTVDGRALTTEDPAPEGWI
                     NATVPGTVLTTLVNNSLMPEPWYGMNNETIPDVWDTGRDYYTYWFFTRFSIPGLDSTR
                     QVWLNFRGINYRAEIWVNGSLLSDTATDGMFLRRKYNVTSLLNPGGHNRLAVRVEPPL
                     NPGMPNGGQGGDGTIGRDVTMQYTAGWDWIQPVRDRNTGIWDAVTIEITGDIDIRNSF
                     ARTRVPGERLPGELQDPAFVTFSAELVNPTGRIVEGEVAVSYMGSTDRKKIKLPPQST
                     VTYTFPEQKQTDPRIWWPNGLGQPALYPAVITFSDRKGNTIDREDLMFGFREAGSYFD
                     DSTGARVFTINGQKLFIRGANWIASDGMLRLSPERYQAEVKMHAEMNMNMIRVWGGSI
                     TERPDFYEACDRNGILVWQDLWITGDCNGRWPDTVRKADSQEVRRMYPDDDTLFLRSV
                     EDQVKMVRVHPSLYLICGGNEFPPPAELDTLIRKKLEELDGTRIYLSESTSPELLRNT
                     IGGTADGPYNIREPLWFFTEKWHPFNPEIGSVGLPNIESLRKMMDEKDLVVPVGGEVN
                     EVWRYHKYMGYGRMIERLGEPSDLADFVRKAQLLNYEQYRALVEGYTAHMWDWYTGFL
                     VWKSQNPWPALKGQFYDWYLDQNAGFYGFRHGASPVHLMFNPVDSAIYIVNTTPKERK
                     GLRLEAKLTDEYGREIWKKAEEKVVDGNSIVKVWKPDLAGSPAKVQFLKLRITYVSTG
                     LTVDENTCWFPQDGDRKNLTSLPEAKIVGQMMKNKDGKVSVDLANSGNVAAFFVRMKV
                     LRASDGTMLTPVFFDDNYIVLLPGEKRTVQVDVTGVSVEERNTPLLLQLEGFNLQPVM
                     IRL"
     gene            4254..7223
                     /locus_tag="PKK03_04940"
     CDS             4254..7223
                     /locus_tag="PKK03_04940"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNX83775.1"
                     /translation="MHPPIVAARERLFALILTQHRRWGTILMPYMLEKMPGREYYTSG
                     EALSPFPNNLTLSGLDNEEKEAVRLVNEYSDRNLFKLFSKHRNVKEFLENVAEKEFSA
                     LIKPFMESRIAQCLQITLKEEIPLFLQKPGVNTLHHEDRLKINPLKADPVFRFDRTPG
                     ETAYSLIVESAGKPVNLRGQQIEILSNMPCIIRSGHTIHFIAEIEGTKIKPFLTRDRI
                     IIPEKTVEKYFSTFVLQVINSHRVTGTGFSVKHSEADKKAFLSVERTLTGVPAAILKF
                     RYDNREIYYPDDITHFTTFSNEEGHISFLRKERDPAWEKSCLDALDEAGLTSDDGINF
                     TTPGTREAEMETMYNLIETVSYNRKRLEDAGLIIRTGSLDRPYSLHRVEIRISHKVIN
                     DWFDLKAEVIIGPFTIPFTRFRRNILEGIREYLLPDGTVALLPEEWFARYRGLFEMGK
                     DSNDDLLLHKQHFSIFSEAVSSEECDACTGLEKLVMPEKLEILPAPEGLNAELRPYQQ
                     EGMSWLHFLQSNNLGGCLADDMGLGKTLQTLALLMFNRERMKARMISQNCPEPGADGS
                     DLTQLTLFGREAPPATSLIIVPASLCHNWHNEIKRFCPSMKTLIHYGATRHRSASHLS
                     DYDIVLSSYHTVRQDIDLFTRVCFFYVVLDESQHIKNPSSHLYRSVMRLRADHRLVLT
                     GTPVENSLTDLWTQLNFVNPGLLGSLSFFRREFARPIEKNYEEEKENRLRKIIQPFIM
                     RRTKEMVVSELPPVTEQVIQCDMSDEQAAVYEKEKSAVRNTILENIESVGIEKSAIIV
                     LQGLMRLRQLANHPVMTDENYNGGSGKFDAVTHNIGNVVAEGHRILIFSSFVKHLELF
                     PGWLEKNGIGYTMLTGSTRERGKVIGNFRNNENIKVFLISLKAGGVGLNLTEADYVFI
                     LDPWWNPASEIQALSRAHRIGQEKRVFVYRYISGNTIEEKIQRLQEKKSRLAGTFIDA
                     NNPLSDLGVNEMLEILN"
     gene            7274..8164
                     /locus_tag="PKK03_04945"
     CDS             7274..8164
                     /locus_tag="PKK03_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83776.1"
                     /translation="MNTYQNHPLAGAVDIDSAFNKLWYFYKKYFLGLYIISVAGSLVS
                     SLFISGLDLTSLQTTTDPAEMLEKMKGFAGPYALALLVTLVLSVLLHTWVLERPAGDA
                     GFTSALLKKSLVAFVPYLVAMIIFGVLTMMLVSIGIVLLVLPGLFAIFYMATAGLFVM
                     PVTLAETRNPLEALTRSFRLTHKNLWANMGWVIVVLLILIVASLVLGAIVMLPFTGSF
                     LRSMANPEEATAMLEMARNPVYIGLSALTGGLVTPVMPILAFLLYFRNRGEEVVVEVT
                     TDSDTTVKVEDLYPPMPPRE"
     gene            complement(8196..9932)
                     /locus_tag="PKK03_04950"
     CDS             complement(8196..9932)
                     /locus_tag="PKK03_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HNX83777.1"
                     /translation="MHKLNDFLTMLDGYLGGHVWFMVLLIGTGLFFTIYLGFPQIRYF
                     RHAIRITKGKYDHSHDIGDTSHFQSLATALSGTVGTGNIAGVALAIHLGGPAALFWML
                     LTAAIGMCTKMVEVSISHKYRDILPDGSVSGGPMYYMKKRLNITTKNGKVLKTGLILG
                     SFFAGATILCSFGTGGLPQINSISNAVFATFGVKHIITGAILSVLLALIIIGGIKRIA
                     KVTSVLVPFMALLYLVGSLIVIFSNVENILPSLVAIFSDAFTGSAAAGGFLGASFAFA
                     MNRGVNRGLFSNESGQGSAPIAHSAARAPEPVSEGMVALLEPFIDTICICTLTGLVVL
                     SSGAWNTKTENHFQTTDMQYVVGAYDDSRSEDVVKLGDHVRGTAPLPLFSGQMEVENG
                     ISKTPGISLIHARSLAENVKYAAGNAPFTGVIEIRDGKLANPAAFDIVGESLIHSAPM
                     TTFAFSRSVMKGWGKYIVAFGLLLFAFSTAISWSYYGDRAVTFLAGPKYVIWYRIIFV
                     VAFFIASFTDTTIVWSLSYVAIVLMAVPNLIGILILRKEMKQSVKDYWLTFAKQYPEE
                     KISGKMLKRFSR"
     gene            complement(9951..11315)
                     /locus_tag="PKK03_04955"
     CDS             complement(9951..11315)
                     /locus_tag="PKK03_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNX83778.1"
                     /translation="MSGFDFSKRDSFGSRLGVVAAAAGSAVGLGNIWRFPYILGENGG
                     GAFLIIYLVIMFAIGIPVMMSELVIGRRSQRNAVGAFRMLAPGKPWYLVGMMGIISAF
                     MILAFYTAVSGWTLEYLYQTVIGGFKGKSSAELTAMFDSFRSDSLRPALWFAIFMLAT
                     SGIVLGGVRKGIEKSTKIMMPLLFVLLIIMCIKSLTLPGAGKGMDFLFRPDFSKITGT
                     AVLMALGQVFFSLSIGMGTIITYGSYIQKDNNLATTSVQVSLIDTTVAVLAGIAIFPA
                     VFAFGISPASGEALTFIVLPGIFQQMPGGMVFSFMFFLLLALAALTSTISVLEVIVAY
                     FSEQLGLSRRKAIVLATISMFILGITASLSMGLTSGIKLFGLNTFDLFNTTTANILLP
                     LGGLLIVIFLGWFFPGKDTRDELSNGGQLKIRYYSIYRFIIRFVAPIAITIVFLNGIG
                     LLKI"
     gene            complement(11430..13172)
                     /locus_tag="PKK03_04960"
     CDS             complement(11430..13172)
                     /locus_tag="PKK03_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HNX83779.1"
                     /translation="MHGLNDFLVKLDGYLGGHPWFMVLLIGTGLFFTIYLGFPQLRYF
                     RHAIRITRGRYDHSHDVGDTSHFQSLATALSGTVGTGNIAGVALAIHLGGPAALFWML
                     VTAAIGMCTKMVEVSISHKYRDILPDGSVSGGPMYYMKKRLNITTRSGKVIKTGVIMG
                     AFFAGATILCSFGTGSLPQINSISNSVFASFGIKHIITGAILSVLLALVIIGGIKRIA
                     KVTSVLVPFMALLYLTGALLVIFSNPENIIPSFVSIFADAFTGSAAAGGFLGASFAFA
                     MNRGVNRGLFSNESGQGSAPIAHSAARAPEPVSEGMVALLEPFIDTICICTLTGLVLL
                     ASGAWNTKTDNQFQPTDVQILAGVYNDGNSEDVMKLGNHMRGDEFLDLYNGKLRIEEG
                     VISTAGISVIHARSLAENVRFLSGRDPFNGELEVVAGKLAPVSTLTVRGESLIHSAPL
                     TTYAFSRSVLKGFGSYIVTFSLLLFAFSTAISWSYYGDRATTYLFGQKYVLIYRLIFV
                     AAFFIASFTDTTIIWSLSYIAIVLMAVPNLVGILLLRKEVKQSVRDYWLAFSNQYPGE
                     KISKKMSRRFGRKQ"
     gene            complement(13190..14554)
                     /locus_tag="PKK03_04965"
     CDS             complement(13190..14554)
                     /locus_tag="PKK03_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNX83780.1"
                     /translation="MSGFDFSKRDSFGSRLGVIMAAAGSAVGLGNIWRFPYILGENGG
                     GAFLIVYLIIIFSIGIPVMMSELVIGRRTQRNPVGAFRMLSSRRPWYLVGMMGIVAAF
                     MILAFYTAVAGWTLEYIYQILAGGIKGRSSAELTVMFDTFRTDSLRPALWFSVFLLAT
                     AGIVLGGVRKGIEKSTKVLMPLLFILLIFMCIKALTLPGAGKGVEFLFRPDFSKITGT
                     TVLMALGQAFFSLSIGMGTLITYGSYIPKDNNLAGTAVQVSMIDLLIAVLAGLAIFPA
                     VFAFGISPASGEALTFIVLPGIFQQMTGGMIFAFIFFFLLAIAALTSTISVLEVIVAY
                     FSEQLNLSRRTAIIIATASMFILGIAASLSWGVMKGVTLFGLNIFDLFNYTTANILLP
                     IGGLLIVAFLGWFFPGKSVRDELSNDGSLKIRYYSLYRFIIRFIAPVAIALVFLNGLG
                     LLAF"
     gene            complement(14703..16484)
                     /locus_tag="PKK03_04970"
     CDS             complement(14703..16484)
                     /locus_tag="PKK03_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HNX83781.1"
                     /translation="MVTLKINDQEVKVAEGTTVLDAAKQLGIIIPTLCNHPDLCVAGN
                     CRVCVVEQKGARTLIAACAMPAAEGMDIHTNSLKVRNARKHIVELLLSEHRADCTKCY
                     KNQKCELQTLANEYAFGEHLFLDLVEDHAYTIDRSSPSFLKDDSKCIRCQRCVRTCAQ
                     LQHVSAIAVANKGAHMKISSFHDRPMSHVVCTNCGQCVNRCPTGALTEKTYIDQVWDA
                     IYDSEKHVVVQTAPAVRVALGEDLGYDPGERVTGKMVTALRQLGFDSVLDTDFTADLT
                     IIEEGTELLTRLKKNLVENDHSMALPMFTSCSPGWIKFIEHKYPHMLSNLSTCKSPQQ
                     MFGALAKTFYAQKRKLDPSKVVSVSIMPCTAKKYEADRPEMRSSGYKDIDFVLTTREL
                     AVMIKQAGIDFRTLEPSHYDSIMGTSTGAAVIFGATGGVMEAALRTAYELVTGKPVPF
                     NNLNITPVRGMEGVKEATVKIEGTTQEWRFLEGAELKVAIAHGLVNANKVAKAVSTGK
                     ASYHFIEIMACPGGCIGGGGQPIPTNAAVRQKRMDAIYSEDMHMDIRKSHENPEIKAI
                     YEEFLEKPNGHKSHELLHTHYIAREAY"
     gene            complement(16502..17662)
                     /locus_tag="PKK03_04975"
     CDS             complement(16502..17662)
                     /locus_tag="PKK03_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNX83782.1"
                     /translation="MKSKGLTKVDLIFESIDAKEVLAKAFSMTPEEIINQVLESGLKG
                     RGGAGFPTGMKWKFTAAEKDPEKYIVCNADEGEPGTFKDREILDRVVYKVYGGMAIAG
                     KAMGAKKGYIYLRGEYRFLLPQLYLELHKFQKMIAEIGYDFTIEYRMGSGAYICGEES
                     ALFESIEGFRGEPRNKPPYPTTSGLFSKPTSINNVETLVTAMMIIAKGPQEYVKLGTK
                     DSRGSKVFSISGDTPIPGIFELELGMTVQEFVNEFGDGDTKAVQVGGASGFCVPRKKF
                     EDTIIGFEGVPTGGSMKLFNSSRSMYNVLHNYLDFFTEESCGQCTPCRVGCQQLLKGV
                     EKVKKGEAKSTYLNELVKLADTMKVASKCGLGQSVGNAFRSIVENFREEIIY"
     gene            complement(17678..18142)
                     /locus_tag="PKK03_04980"
     CDS             complement(17678..18142)
                     /locus_tag="PKK03_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNX83783.1"
                     /translation="MYQTGNMVKELADKYGRKRESLMPILQDIVKKHNYLTDEAMVEV
                     ATELDISAAEVYGTASFYTFLDTEERGKNVIRVCKTITCSMKGKNDVISTLEEILKIK
                     VGQTTPDKMFTLLETNCIGWCHKAPAMLVNDMPYTDLSSETVIDVIKGYMRK"
     gene            18410..19252
                     /gene="kduI"
                     /locus_tag="PKK03_04985"
     CDS             18410..19252
                     /gene="kduI"
                     /locus_tag="PKK03_04985"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HNX83784.1"
                     /translation="MKTNIEVRYAAHPDDVRHWDTDRLRREHLVSAVFQDNAVTMVYS
                     LYDRMVAGGAKPAGEALKLEPVDEVKAAFFLHRRELGVFNVGGPGRVTAGGAEYALEY
                     KEALYLGCGDREVTFESSDAANPALFYFNSAPAHRNYPDRKVTKKDCITAEMGSAEAA
                     NHRIINKMIVREVLETCQLQMGMTELKPGSVWNTMPPHTHGRRMEVYFYFEVPDGSVV
                     CHFMGQPSETRHIWMKNNEAVISPQWSIHSATATSNYTFIWGMAGENLDYGDQDFLKQ
                     TDLR"
     gene            19281..20066
                     /locus_tag="PKK03_04990"
     CDS             19281..20066
                     /locus_tag="PKK03_04990"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HNX83785.1"
                     /translation="MNSNLFRLDGRVALVTGASYGIGMAMAKALAEAGATLVFNDRNP
                     DRVTEALAGYRKSGIDARGYVFDVTDETAVREYIEKITSEIGVIDILVNNAGIIKRIP
                     ALEMDAADFREVIDIDLAGPFIMSKAVAPGMIRKGSGKIINICSMMSEVGRETVSAYA
                     SAKGGLKMLTRNLACEWAEYNIQVNGIGPGYIATPQTAPLRTEGHPFNSFIIGRTPAA
                     RWGTPGDLQGPVVFLASPASDFVNGHILYVDGGILAYIGKQPR"
     gene            20079..21206
                     /locus_tag="PKK03_04995"
     CDS             20079..21206
                     /locus_tag="PKK03_04995"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HNX83786.1"
                     /translation="MQKLSLFLISLFLLAGCSGNRMKVTLDNALSIDRFAETVEIPIS
                     DIASRLGDIDPAALVVTDSKGAEVPSQIIRAGQAEPLSLIFQADVKAGSSARYYIASG
                     TPQSYEPKAYGRFVPERYDDYAWENDRIAFRIYGPALIAKDGPSNGLDVWIKRTGKMV
                     IDRWYSDYLAGINTYHDDNGEGCDCYKVGRTLGAGAMAPYVDDSLWLAINFENWKTLD
                     NGPVRTSFRLTYPPFLVRDVMVTETRTISLDAGSQLTKITEEYTGIDKEFTVAAGIVR
                     REEGSPLLFPPEKNAIAYRLDGGEGGITYIGTVLTTPATAVEDIADHLMIVTHYRPGT
                     PLTYYTGAGWSKWGFETDAAWTRYVNEFSQKLASPLKVKTE"
     gene            21243..>23266
                     /locus_tag="PKK03_05000"
     CDS             21243..>23266
                     /locus_tag="PKK03_05000"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNX83787.1"
                     /translation="MLLLMVTAALRTGCGQFKSAFTHYSVEDGLSEGVVLTMHQDREG
                     LMWFGTFDGLNRFDGYNFKVYKSGYNSAYGLTNNRVDRITEDDLGYLWIMNNDGEVYR
                     MNTRSEEFINFNEISSSGSGNYSPVNQICCFSGGETWLQAEAGTCIRVISSASSPGYT
                     IDRFNCQGSDNTPVKVNLISRDSRGTAWILTSVGLFRVRGDDKVPVRALTPGIRPEGR
                     NGFFCIHEAGDRLWFGTGNGTIIITDAEAGAYEELSLGVRSDIISIRQLNQRDLLILT
                     SGDGFFVYSPVTAEKKQFDTSRYPAMPTNSMVSAYVDRYGEAWIESAAAGVVHYDPFT
                     GTVRHFRMNVDKTNPNVLLPSFVIFEDVNDMLWIYPRGGGFSRYNRVERRLEYFFNEP
                     GGADRRFPNLIHSALSDNQGNLWMCTITRGIEKVSFFPNLFRVLSPKPVAASHSENEV
                     RAIFEDNNRHLWAATRDGRVYLFDPAKKMVGYLRDNGTFSSGEAFNALVYVITQDSRG
                     SIWIGSKGGGLFRLEPVTKGPAYSYRITGYRHDDADPYSLSHDNVYSVHEDRGGRIWV
                     GTFNGGINYISEEGGRVRFINAGNRLKNYPYSNHKRVRYITSDNHGRIWVGTTSGLVI
                     FSRDFAEPEDAEFTTCFFNPDVETSLANNDVHYIYCSPDDEIFVATFGGGL"
     assembly_gap    23267..23366
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23367..>25518
                     /locus_tag="PKK03_05005"
     CDS             <23367..>25518
                     /locus_tag="PKK03_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX83788.1"
                     /translation="LRTSDSEIMFGFDNGIYCFNPAQVRKVNYVPPLQFTRLLVAGTD
                     VHTTGGNYNRPGEKGEQATLELKHDQKSLTIEFAALDYRNPKNIQYAHRLEGVDPDWI
                     IARGQRTASYTNLDPGEYLFSVRSTNSDGIWVDNTRTVRVTVKPSFWQTLFARLLIVL
                     AGAALIALAIYVIFTMYKLRHKVEVEQIITNLKLKFFTDISHELRTPLTLISSPVTNI
                     LQNNRLEPEVRDQLTLVQNNTQRMLRLVNQILDFRKVQNRKMKLRIEEFPLGPFIKGI
                     CADFRKAAEDKGIRFTVDDLSEGEKVWADRDKVEKIVFNLITNALKFTPRGKNVTVTV
                     RKNSDSLELIVADQGTGLSREKIKNLFMRFSSDEGEVTTLQPGTGIGLSLSRELAELH
                     RAELTAESEEGKGATFRVIFRLGFSHFDNETEFVLSDGQNPLRTRQTPSAMQEEDEEN
                     EAVEGDDEPVLLFIEDNEELRSFIKIILSDGFHVIEAGNGIEGFEIARNQLPDIIITD
                     LMMPEMDGLELARRIKEEVTTSHIPVVVLTAKSDLDTQVEALKRGADDFITKPFSSTY
                     LRARIENILRQRKKLQELFLSSLDDYHRPKNGKPVDITPSEPVIESYDDRLMKNLMEV
                     MEENIDNSALTVDELVSKMGIGRSVFFKKLKSLTGLAPVEFIREVRVKRAAQLIESGE
                     YTISQVTYMVGCNDPRYFSRIFKQRFAMTPTEYRD"
BASE COUNT         6441 a         6768 c         6329 g         5880 t
ORIGIN      
        1 acctcgtgcg tcatctcaat acccgccttc cggtaaacag ccgagacccc gcaatagcgc
       61 tccatcgaaa ggctgatcgc cttctctatc ttttccatct cgaggtccct gccactgaac
      121 tcataaatga tatgcatcct gacataggca ttcggatgct cgtcctcagt atctccttcc
      181 acaacaacgt tgaagtactc cacctcctgt ctcatttttt tcagaatcga tatcacgtcc
      241 atccccgtgc aaccagccag agctgccagc atgagcggtt tcgggcgagg tccgctgtta
      301 tcaccgtttt caccggcctg gtcaataaaa aagtgatgcc cgttaacttc agtgtcaaat
      361 ttcattttgc ctttccaggc agattctatc ctgtgtttca ttgagtcggt ttgtttgacg
      421 caaaagtaca tcctcccagg actctcctgg ccatggcaac ggccggacag atcatgacaa
      481 ttattgaaat gcgacaacaa aaaagttgct gtacttcagc ctcatgaagc caagttactg
      541 attattataa aattgacgac accccactga taatgtgcgt taattcttaa atttgcacgt
      601 cactcaattg taatgaagac catcaccaat actgacaggg agtttgtctg cgacatcacc
      661 ctgccctgtt tctcggcact tacgcctgag gagacagagc tggttcgcga aagcagggtt
      721 caggtcgttt ttcgccgtgg cgagaacctg gccaaacagg gagcttttgc ttcatatgtt
      781 atcttcactg tcaccggtta ctcgaggcag tatatcgagt gcagcaacgg gcgcgactat
      841 aatctccgga tcatccgtca gggcgaaatg gtagggctct cggcggtctt taaccggaat
      901 accttcagtt attcagtggt agcagtcact gagacaacag gtttcctgat agagaaggat
      961 gccatcgcgg cgctggtgaa gcagaacggg acatttgcct tcagcatcat caggcggtac
     1021 tgtgagcaca actcgctgct ctatacctca ctcgacaatg tgctgcatca gcagaccaat
     1081 gggcgtatgg cgacggtgct cctgagcctg gcccctgaga acttcggcgg tgaggatctc
     1141 ttcaggcatc tctcgcggcg tgagcttgct gacttcgccg gcatctccac cgagggagca
     1201 gtgaaggtgc tgaagagttt cgagaaggac gggctgatca aactctccga taagagtatc
     1261 actttaacca accgcccgcg aattgaggag atcagccgca cagggtaagg accatatccg
     1321 taaatggtaa ggaccggatc tgcaaagggt aaggatctga tccgtacagg acaaggatcg
     1381 gatccgcaca cgataagatc cgttgccggg ccggccggaa ttaaacacta aagcctgatc
     1441 atcaccggct gcagattgaa tccctccagc tgcagcaaca gaggggtgtt cctctcttcc
     1501 acactgactc cggtcacatc aacctgtacc gtgcgctttt ctccgggtaa caacactata
     1561 taattgtcgt caaagaaaac cggtgtcagc attgtgccgt cagaagcccg caacaccttc
     1621 atccgaacga aaaaggcagc aacgtttccg ctgttggcca ggtccacgct gaccttgcca
     1681 tccttgttct tcatcatctg gccaacgatc tttgcctcag gcaacgaggt cagattcttc
     1741 ctgtcaccat cctgcgggaa ccagcaggta ttctcatcaa ctgtcaagcc ggtggaaaca
     1801 taagtgatgc gaagcttcag gaactgaacc tttgccgggc tgcctgcaag atcaggcttc
     1861 cagactttca ctatgctgtt tccgtcaaca accttctcct ccgccttctt ccaaatctcc
     1921 ctgccgtact catcggtcag cttcgcctca agcctgagcc ccttgcgctc cttgggcgtg
     1981 gtgttgacaa tgtagattgc agagtcaaca ggattgaaca tcaggtgcac cggtgaggca
     2041 ccgtgcctga agccgtaaaa acccgcattc tgatcaagat accagtcgta aaactgtccc
     2101 ttgagcgccg gccacggatt ctggctcttc cagaccagga aacctgtgta ccagtcccac
     2161 atgtgcgctg tgtatccctc aacaagagcc cggtactgct catagttcag caactgtgcc
     2221 ttcctgacga aatcagccag gtcagagggt tctcccagcc gctcaatcat cctgccgtaa
     2281 cccatatact tatgataccg ccatacctcg ttcacctcac cacccacagg cacgacaaga
     2341 tctttctcat ccatcatctt cctgaggctc tcgatgttag gcaatcccac tgaccctatc
     2401 tccggattga acggatgcca tttctcagtg aagaaccaga gcggctccct gatattatac
     2461 ggtccgtcag cagtcccgcc gattgtattc cgcaagagct ccggcgaagt tgactcgctc
     2521 aggtaaatcc gtgttccgtc aagctcctca agcttcttcc ggatcagggt atcaagttcc
     2581 gccggtggcg ggaactcgtt tcccccgcag ataagataca gggacggatg aacccttacc
     2641 atcttcacct ggtcttccac ggaccgaagg aaaagagtat catcatcagg atacatacgc
     2701 cggacctcct ggctgtccgc ttttctgacg gtatcaggcc acctcccgtt gcagtcgccg
     2761 gtgatccaca ggtcctgcca caccagaatg ccgttcctgt cacaggcttc atagaaatcc
     2821 ggcctctcgg tgattgatcc gccccagacc cggatcatgt tcatattcat ttcggcatgc
     2881 atcttcacct cagcctggta acgttccggg ctgagacgca acattccgtc cgaagctatc
     2941 cagttggcgc cgcgtataaa aagtttctgg ccgttgatgg taaagacccg tgccccggtt
     3001 gaatcatcaa agtagcttcc cgcctcacgg aatccgaaca tcaggtcctc ccggtcaatc
     3061 gtgttgccct ttctgtcact gaaggtaatc acggcaggat acagcgccgg ctgacccagg
     3121 ccgttgggcc accatatcct cggatcagtc tgtttctgtt ccggaaaggt ataggtcacg
     3181 gtgctttggg gcggcagttt tatcttcttt ctgtcagtgg atcccatgta tgaaacggct
     3241 acctcccctt ccacaatcct gccggtcggg ttcaccagct cggcggagaa ggtcacgaag
     3301 gcagggtcct gcaactcgcc ggggagccgt tcaccgggaa ccctggtacg ggcaaagctg
     3361 tttcttatgt caatatcacc tgtgatttca attgtcaccg catcccagat ccccgtattc
     3421 ctgtccctga ccggctgtat ccagtcccac cctgccgtat actgcatggt cacatcacgg
     3481 ccaatggttc cgtcgccccc ttgtccgccg ttgggcattc ccgggttaag tggcggctcc
     3541 accctgacag ccaaccggtt atgccccccg gggttcagaa gggaagttac attgtatttc
     3601 ctgcgtagaa acataccgtc agtagcggtg tcactgagga ggctgccgtt gacccagatc
     3661 tcggccctgt agtttatccc cctgaaattg agccacacct ggcgggtgga gtcaagtccc
     3721 gggattgaga accttgtgaa gaaccagtat gtgtaataat cgcgacccgt atcccatacg
     3781 tcaggaatag tctcgttatt cattccgtac catggttccg gcataaggct gttatttacc
     3841 agtgtagtca ggacagtgcc gggaacagtg gcatttatcc acccctccgg tgcagggtcc
     3901 tctgtggtca gcgccctgcc gtcaacagtt aattccgatg cgcggattgc cctccatccg
     3961 gttgtgatag gtgtctcctg agaggaggcc agaaaagtca aactcaataa aaaactggtt
     4021 ataatgacct ttttcatctg ttgttccctt attaaacaag tacggaaaga tattaaaatt
     4081 atcctttcgc agcgcggcag acagggcatg aatgccgtaa tcatctcaca ttcagtgttt
     4141 aatatcgaca aagtcacaga taacctgacc ggagccgggg aaataattat ttaatgccag
     4201 ctatttgatt ttatccgggg aatttcaaaa ttgcatgttt atttcgttta cagatgcatc
     4261 cccccatcgt tgcagcccgg gaaaggctgt ttgcactgat actgacccag caccgcagat
     4321 ggggcactat cctgatgcca tacatgctgg agaagatgcc ggggagggag tattacacct
     4381 ccggcgaggc gctctcaccc ttccccaata acttaaccct ttcaggactt gataacgagg
     4441 agaaggaggc ggtacgactg gttaacgagt actcggaccg caacctcttc aagctttttt
     4501 caaaacaccg caatgtcaag gaattcctgg aaaatgttgc cgagaaggag ttcagcgcac
     4561 tgataaagcc atttatggag tcaaggattg cgcagtgtct ccagataacc ctgaaggagg
     4621 agataccact cttcctccag aagccgggag taaacaccct gcatcatgaa gacaggctga
     4681 aaatcaatcc cttaaaagca gatcccgtgt tccgttttga ccgcaccccc ggagaaaccg
     4741 cgtacagtct gatagtggaa tcagccggca aaccagtcaa ccttcgcggc cagcagatcg
     4801 agatactgtc aaacatgcca tgcatcatca ggtctggaca taccatccac ttcattgctg
     4861 aaattgaagg gaccaaaatc aagccgttcc tgaccaggga caggatcatc attcctgaga
     4921 aaacggtgga aaaatacttc tcgacgttcg tgctccaggt gatcaacagt caccgggtga
     4981 caggaaccgg gttctcagtt aaacactcag aagcagataa aaaagcattt ctcagtgttg
     5041 agcgtaccct cacaggggtt ccggcagcca tactgaaatt ccgctatgac aacagggaaa
     5101 tttattatcc tgatgatatt acccatttca caaccttcag caacgaagag ggccatatca
     5161 gcttcctgcg gaaggaacgg gatccggcat gggaaaagag ttgcctggat gcgcttgacg
     5221 aagcgggcct gacaagtgac gacggcatta acttcaccac cccggggacc agggaagcgg
     5281 aaatggaaac aatgtacaac cttattgaaa cagtctccta caacaggaag agactagagg
     5341 atgcgggtct gataatccgt accggcagcc ttgaccggcc atactcgctg cacagggttg
     5401 agataagaat cagtcacaag gtcataaatg actggtttga cctgaaagcc gaagtgatca
     5461 taggaccgtt taccattcca ttcacgcgtt tcaggaggaa cattcttgaa gggatccgcg
     5521 aatacctgct gcccgacgga acggttgccc ttctccctga ggaatggttt gccagatacc
     5581 ggggactgtt cgagatgggc aaggacagca atgacgatct gctgctgcac aaacagcatt
     5641 tcagcatctt cagcgaggct gtctcctcgg aagagtgcga cgcctgtacc ggactggaga
     5701 agcttgtcat gcctgagaag ctggagatat tgcctgcacc ggaagggctt aatgcagagc
     5761 tgcgacctta ccagcaggag ggaatgagct ggttacattt cctgcagtca aacaacctcg
     5821 gcggttgcct tgctgacgac atgggcctcg gcaagacact gcagaccctg gcgctgctca
     5881 tgttcaacag ggaaaggatg aaagcccgca tgataagcca gaactgccct gaaccgggcg
     5941 ctgacggcag tgacctgaca cagctcaccc tctttggcag agaagctccc ccggccacct
     6001 ccctgatcat tgtaccggca tcgctctgcc acaactggca caatgagata aaacgtttct
     6061 gcccgtcaat gaagacactc atccactacg gcgcaacacg gcacaggtca gcgtcacacc
     6121 tctccgacta cgatattgta ctgtcaagtt atcatacggt caggcaggac attgatctat
     6181 tcaccagggt ctgcttcttt tatgtggtac tcgatgagag tcagcatatc aagaacccct
     6241 cctcccacct ctacaggtcg gtgatgagac tgagggccga tcacaggctg gtacttaccg
     6301 gcactccggt agagaactca ctgaccgacc tgtggacaca gcttaacttc gtcaatcccg
     6361 gtctgctggg atcactctca tttttcagga gggagtttgc aagaccaatc gaaaagaact
     6421 acgaggaaga aaaggagaac aggctcagga agataatcca gccgttcatc atgagacgca
     6481 ccaaggagat ggtggtgagt gagcttcctc cggtaacgga gcaggtcata cagtgtgaca
     6541 tgtcagacga acaggctgct gtttatgaaa aggaaaaatc tgctgtccgt aacaccatcc
     6601 tggaaaacat agagtctgtg ggtattgaaa aatctgccat catcgttctc cagggactga
     6661 tgaggctccg gcaactggca aaccaccccg tgatgacaga tgagaattac aacggaggct
     6721 cgggcaagtt tgacgccgta acccataata tcgggaacgt ggttgctgag ggacaccgga
     6781 tactgatatt ctcatcattt gtcaagcacc ttgagctgtt ccccggatgg ctggaaaaga
     6841 acggcatagg atacacaatg ctcaccggtt ctaccaggga acgcgggaag gtgataggca
     6901 acttcagaaa caatgaaaac ataaaggttt tcctgatttc actcaaggcc ggaggagtcg
     6961 ggcttaacct tacagaagcc gattatgtat tcatcctcga cccgtggtgg aatcccgcat
     7021 ccgagattca ggcgctcagc agggcgcaca ggataggcca ggagaaaagg gtctttgtat
     7081 acaggtatat ctccggcaac accatcgagg agaaaatcca gcggttgcag gagaaaaaat
     7141 cacggctggc cggaactttc attgatgcca acaatccact gagcgatcta ggcgtgaatg
     7201 aaatgcttga gatacttaac tgatttttta atagatttac ctctgaattt acaaatcttc
     7261 taaaaacgct gcaatgaaca cctatcagaa ccatcctctt gccggggctg tcgatattga
     7321 cagtgcattc aacaaactgt ggtacttcta caagaaatac ttcctggggc tttatattat
     7381 atccgtagcc ggatcgctgg tttcatcgtt attcatctca ggcctcgacc tgacatcact
     7441 tcagactacc actgatcccg ccgagatgct cgaaaagatg aagggctttg caggacccta
     7501 tgccctggct ctcctggtaa cactggtact gagcgtattg ctgcatacat gggttctgga
     7561 gaggcctgcg ggagatgcag gcttcacctc tgccctgctt aaaaaatcac tggttgcgtt
     7621 cgtgccgtac ctggttgcca tgatcatctt cggagtgctg acaatgatgc tggtgtcaat
     7681 cggaattgtt ctcctggttc ttccggggct ctttgccatc ttttacatgg caacggcagg
     7741 tcttttcgta atgccggtaa cacttgccga aacccgtaat cccctggaag cacttacacg
     7801 ctccttcagg ctgacgcata aaaacctgtg ggccaacatg ggatgggtta ttgtcgtcct
     7861 ccttatcctg attgtcgcat cactggtact tggagcgatt gtgatgcttc cgtttaccgg
     7921 atcattcctc aggtccatgg ccaatcccga agaggctacg gcaatgcttg agatggcacg
     7981 caacccggtc tacatcggcc tcagtgccct gaccggcggc ctggtgacac cggtaatgcc
     8041 gatacttgct ttcctgctct atttcaggaa caggggcgaa gaggtggttg ttgaggttac
     8101 caccgacagt gataccactg tgaaggttga ggacctgtac cccccgatgc ctcccaggga
     8161 gtaacagcgc ccggaacgga acaggaaagc ctgctttacc tgctgaacct tttcaacatt
     8221 ttgccggaga tcttctcttc cgggtactgc ttcgcgaagg tgagccagta atccttaacg
     8281 ctctgtttca tctccttgcg gaggataagt atcccgataa ggttggggac agccatcaat
     8341 actattgcca cgtatgaaag tgaccatact atggttgtat cggtgaatga tgcaataaag
     8401 aatgccacca cgaagatgat gcggtaccag atgacatact tcggtcccgc caggaaagta
     8461 accgccctgt cgccatagta ggaccacgaa atggcagtgg agaatgcaaa aagaagtaac
     8521 ccgaaggcga ctatatattt cccccagccc ttcatcacgc tccggctgaa ggcaaacgtg
     8581 gtcatcgggg cactgtgtat gagtgattca cctacaatgt caaatgccgc aggattggcc
     8641 agcttgccgt ccctgatttc aatcacccct gtgaacggtg catttcctgc agcgtattta
     8701 acattctctg cgagggatct tgcgtggatc agtgatatgc cgggagtctt tgaaataccg
     8761 ttctccacct ccatctgtcc gctgaaaagt ggcaacgggg ccgtcccgcg tacatgatca
     8821 ccaagcttaa ccacatcctc gcttctgctg tcgtcatacg caccgaccac atactgcatg
     8881 tcagttgtct ggaaatggtt ttcggtcttg gtgttccatg cacctgaaga aaggactacc
     8941 aatccggtca gcgtgcaaat gcagattgta tcaatgaatg gttcgaggag ggcaaccata
     9001 ccctccgaca ccggctcggg agctcttgct gccgagtgag ctattggtgc agatccctgt
     9061 cctgattcgt tggagaaaag tcccctgttc acgcctctgt tcatggcgaa ggcgaaggag
     9121 gcgccgagga agccgccggc agctgctgaa ccggtgaagg cgtcgctgaa aatggcaacc
     9181 agtgacggaa ggatattctc aacgttggag aagatgacaa tgagtgatcc gacaagatag
     9241 agcagcgcca tgaatggcac cagtaccgat gtcaccttcg ctatgcgctt tatcccgcca
     9301 atgattataa gcgccagcag cacggagagg atggctccgg tgatgatatg cttcaccccg
     9361 aaggtggcga agacggcatt ggagatgctg tttatctgcg gcaggccgcc ggtaccgaat
     9421 gaacacagta tcgtggctcc ggcaaaaaag ctgccgagga tgaggccggt cttcagaacc
     9481 tttccgtttt tcgtggtgat gttgagccgt ttcttcatgt aatacatagg ccctccggag
     9541 acactgccgt caggaagtat gtccctgtat ttgtgagata tggatacctc gaccatcttg
     9601 gtgcacatcc cgatggcagc agtcagaagc atccagaaga gagctgccgg tccgccaagg
     9661 tgaatggcca gcgccacgcc ggcaatgtta cctgtaccga cagtcccgga taaagcggtt
     9721 gcaagggatt ggaaatgact ggtatcacca atatcatgtg agtgatcgta tttccctttc
     9781 gtaattctga tggcatgcct gaaatagcgt atctggggaa aaccaagata aattgtaaaa
     9841 aaaagtccgg tgccgatcag cagcaccatg aaccagacat gcccgcccag gtaaccatcg
     9901 agcattgtca gaaaatcgtt tagcttatgc ataaaggtca gggattgcga ttatatctta
     9961 agcagtccta tcccgttaag gaagactatc gttatggcaa ttggtgcaac aaatcttata
    10021 ataaacctgt atatggaata atacctgatc ttaagctgac ctccgttcga aagttcatca
    10081 cgggtatcct tccccgggaa aaaccatccg aggaagatca caatgagcaa tccgccaagc
    10141 ggcaaaagga tgtttgccgt agtggtattg aacaggtcaa acgtattcag gccgaaaagt
    10201 ttaatcccgc tcgtgagtcc catggagagc gaggcggtga tgccgagaat aaacattgaa
    10261 atggttgcga ggacaattgc cttccttcgc gaaagcccga gctgttcaga gaaatatgct
    10321 acaataacct ccaggaccga aatggttgat gtcagggcag ccaatgccag caacaggaag
    10381 aacatgaagg aaaacaccat tcctcccggc atctgctgga agatgccggg gagcactata
    10441 aacgtaagag cctccccgga ggcaggagaa attccaaagg caaatactgc agggaatatt
    10501 gcaatgccgg ccaggactgc caccgtggtg tcaatgagcg agacctgtac cgaagttgtt
    10561 gccaggttgt tgtccttctg aatataggag ccgtatgtaa tgattgtccc catgccgatg
    10621 ctgagagaga aaaagacctg accaagagcc atcagtactg cagtgccggt tatcttcgag
    10681 aagtcaggcc tgaaaaggaa atccattccc tttccggccc ccggaagggt aagcgatttg
    10741 atgcacatga tgatcagcag cacgaaaagc agtggcatca tgattttagt ggatttctca
    10801 atacccttcc ttacaccgcc aaggacaatt cccgatgttg caagcatgaa tattgcaaac
    10861 catagcgccg gcctcaaaga atcgctcctg aaggaatcaa acatggcagt aagctcggca
    10921 gagcttttac ctttgaaacc gccgatcaca gtctggtaga ggtattccag ggtccaccct
    10981 gacactgcag tataaaatgc aagtatcatg aatgctgaga tgatccccat catccccaca
    11041 aggtaccatg gcttaccggg agcgagcatg cggaaggcac cgaccgcgtt ccgctgtgag
    11101 cgccggccaa tgaccagctc cgacatcatc accggaatcc cgatcgcaaa catgattacc
    11161 agatatatta tcaggaaagc gccacccccg ttctccccca ggatgtaggg aaatctccag
    11221 atattaccga gacccacagc agaaccggct gccgcagcaa ccaccccaag cctgctgcca
    11281 aatgagtctc tctttgaaaa atcgaaaccg ctcatatttc agacaatgat cactaatcaa
    11341 aaacaagcaa tcaaagttga ttaaaaactc aatcccttgt atctgagcga taaaaaaaag
    11401 tgttctgtga caaatgcctg cagatcagac tattgttttc ttccgaacct tctggacatc
    11461 ttcttcgaga tcttttcgcc ggggtactgg tttgaaaatg ccagccagta atcacgcaca
    11521 gactgtttta cttcctttct tagcagtagt attcctacaa gattggggac ggccatcagc
    11581 acaatggcaa tgtatgaaag cgaccagatg attgtggtat cggtgaatga ggctatgaag
    11641 aaggcagcca caaagatcag cctgtatatc agcacatact tctggccgaa caggtaagtg
    11701 gtagccctgt cgccatagta tgaccatgag attgctgtgg aaaatgcaaa cagaagcaat
    11761 gagaaggtca ctatatacga cccgaagccc ttcagaacgc tccggctgaa tgcgtaggtt
    11821 gtgagcggag cactgtggat aagcgattcc cctctcacgg tgagagttga caccggggcc
    11881 agtttaccgg ccacgacctc cagttcacca ttgaacggat ccctgcctga aaggaaccgg
    11941 acattctctg ccagtgacct ggcgtgaatg acggatatcc cggcggtgct tatcacccct
    12001 tcctcaattc tgagctttcc gttgtagagg tcaaggaatt catctccccg catatggttc
    12061 ccaagcttca ttacatcctc actgttgcca tcattataca ctccggccag aatctgcaca
    12121 tcagtcggct gaaactggtt gtcagtcttg gtattccagg cgcccgaggc cagcagcacc
    12181 aggccggtaa gggtgcagat gcaaatcgta tcaatgaaag gctccagcaa ggccaccatc
    12241 ccttccgaga caggttccgg ggcacgggct gccgagtgag ctatgggcgc cgatccctgt
    12301 cccgactcat tggagaagag ccccctgttc actcccctgt tcattgcaaa agcaaaggat
    12361 gctccgagga agccccctgc tgccgccgat ccggtaaagg catcggcaaa aatcgaaaca
    12421 aacgagggga tgatgttttc aggattggaa aagatgacca ggagagcgcc ggtaagatag
    12481 agcagcgcca taaagggcac gagcaccgag gtaacctttg ctattctttt tatcccgccg
    12541 atgatcacca gggccagaag taccgaaaga atcgcaccgg ttataatgtg tttgattcca
    12601 aatgaggcaa agaccgagtt tgagatactg tttatctggg gcaggcttcc ggtgccgaag
    12661 gagcacagta tggttgctcc ggcaaaaaag gctcccatta tcacccctgt ctttatcacc
    12721 ttcccgctgc gggttgtgat gttcaaccgc ttcttcatgt aatacatcgg cccgcctgac
    12781 accgaaccgt caggaagaat atccctgtac ttgtgtgata tcgagacctc caccatcttc
    12841 gtgcacatcc cgatggctgc cgtaaccagc atccagaaca gcgctgcagg cccgcccaga
    12901 tgtatcgcca gggccacccc tgcaatattc cctgttccga ccgtgcctga aagtgcggtt
    12961 gcaagcgact ggaaatgcga agtatcaccc acatcatgcg aatgatcgta cctgccacgt
    13021 gttatccgta tcgcatgccg gaaatacctc agctgcggaa atcccaggta gatggtaaag
    13081 aaaagtccgg tccctatcag tagcaccatg aaccagggat gacctccaag gtatccgtca
    13141 agctttacaa gaaaatcatt aagtccgtgc atgtcagtgt ttcagatgat taaaatgcaa
    13201 gtaatccgag cccgttcaga aacacaagag ctatcgccac cggtgcaatg aacctgatga
    13261 taaacctgta aaggctgtaa tacctgattt tcagggaacc atcatttgaa agctcatccc
    13321 tgaccgattt gcccgggaag aaccatccga gaaatgccac aattagcagc cctcctatgg
    13381 gaagaagtat gtttgctgtt gtatagttaa agaggtcaaa gatattcagg ccgaacaggg
    13441 tgaccccttt catcacaccc catgacagcg acgcggctat accgagaatg aacatcgagg
    13501 cagtggcaat tataattgcc gtcctgcggc tcaggttcag ctgttctgag aagtacgcca
    13561 ctatcacctc cagaactgat atggtcgagg tcaacgcggc aattgccagc aggaaaaaaa
    13621 atataaaggc aaaaatcatt cccccggtca tctgctggaa gatccccgga aggactatga
    13681 atgtcagtgc ttctccggaa gcaggcgaga tgccaaatgc aaatactgcc gggaagatgg
    13741 caaggcctgc aagcactgca atcagaaggt caatcataga gacctgcacc gctgtgcccg
    13801 ccaggttgtt gtcttttggt atatatgaac catatgtgat cagggttccc atcccgatgc
    13861 taagtgaaaa gaatgcctgg cccagggcca tcagcaccgt cgttcccgtg atctttgaga
    13921 aatcaggcct gaagagaaac tcaactccct ttccggcacc cggaagtgtc agggctttta
    13981 tgcacataaa aatcagcagg atgaaaagca gcggcatgag caccttggtt gatttttcta
    14041 tcccttttcg cactccaccc agcactatgc ccgctgttgc aagcagaaaa acggaaaacc
    14101 agagggcagg cctgagcgaa tcagtcctga aagtgtcaaa cattaccgtc agttcagcag
    14161 atgacctgcc ctttatgccc ccggcaagga tctggtagat gtactcaagc gtccaaccag
    14221 ccacagcagt gtaaaaggca agtatcatga atgcagccac tatacccatc atccccacaa
    14281 gataccaggg ccggcgcgat gaaagcatcc tgaaggcgcc caccgggttc ctctgggttc
    14341 tcctgccgat aaccagttcg gacatcatca ccggtatgcc aatggaaaag ataataataa
    14401 ggtatactat gaggaacgca cctcctccgt tctcacccag gatataggga aatctccaga
    14461 tatttcccag accaacggct gaaccggctg ctgccattat aactcccagc ctgctgccaa
    14521 aggaatccct ctttgaaaag tcaaatccgc tcatgttttt tattcagttt cagcaattat
    14581 ctgtctgatt ttctaaaaat ggcaaaaaac tccttagcag ccaacttttg tgttgaaaaa
    14641 cagaaaaaaa gcggagacgc catgcaaaac gtctccgcct gacaattgta ctgtctatct
    14701 gattagtaag cttcccgggc tatgtaatgg gtatgcagga gttcatgcga cttatggccg
    14761 ttgggcttct ccaggaattc ctcgtagata gcctttattt cgggattctc atgagatttc
    14821 ctgatatcca tatgcatgtc ttccgagtag attgcatcca ttcgtttctg gcggacagca
    14881 gcgttggtgg gtatgggctg accgccacct ccaatgcatc caccggggca ggccattatc
    14941 tcaatgaagt gatatgaagc cttgccggtg ctcactgcct tcgctacctt gttggcattc
    15001 accagaccgt gtgcaatcgc aactttcagt tcagctcctt caaggaacct ccactcctgg
    15061 gtggtaccct caatcttaac tgtagcctcc ttcacccctt ccatgccgcg gacaggagtg
    15121 atattcaggt tattgaacgg cacaggcttg ccggtaacaa gttcatatgc agtgcgtagt
    15181 gccgcctcca tcacaccgcc ggtggctccg aagatgaccg cggcgcctgt ggatgtaccc
    15241 atgattgaat cataatggct cggctccagc gtgcggaaat ctatccctgc ctgcttgatc
    15301 atcacagcca gttcacgggt ggtgagaacg aagtcaatgt ccttgtatcc gcttgatctc
    15361 atctcaggcc tgtcagcctc atattttttg gcggtgcagg gcatgatcga aacagagaca
    15421 accttcgagg ggtcaagttt cctcttctgc gcatagaatg tctttgcaag ggctccgaac
    15481 atctgctgtg gcgacttgca cgtcgaaaga ttggacagca tatgcgggta cttgtgctcg
    15541 atgaacttga tccatccggg tgagcaggag gtgaacatcg gcagggccat gctgtggtca
    15601 ttctcaacaa ggtttttctt cagcctggtg aggagttcgg ttccctcttc aataatggta
    15661 aggtcagccg taaagtcggt atcgagtacc gaatcaaatc ccaactgtcg cagggcggtg
    15721 accatcttgc cggtaaccct ctcgcccgga tcatatccca gatcctcgcc aagagccacc
    15781 ctcacagccg gggctgtctg tacaaccaca tgcttctctg aatcataaat ggcatcccac
    15841 acctggtcga tgtaggtctt ctccgtcaac gcacccgtcg ggcagcggtt gacgcactgg
    15901 ccgcagttgg tgcagacaac atggctcatc ggcctgtcat ggaacgacga gatcttcata
    15961 tgggcgccct tgtttgccac tgctattgca gacacatgct gcagctgtgc gcaggtacgc
    16021 acgcaacgct ggcaccttat gcacttgctg tcatccttaa ggaaagaagg agatgatcgg
    16081 tcaatggtat atgcatggtc ctcaacgagg tcaaggaaca aatgctcacc gaaagcgtat
    16141 tcatttgcca gggtctgcag ctcgcatttc tggttcttat aacacttggt gcagtctgcc
    16201 ctgtgttctg agagaagcag ctcaacaatg tgtttcctgg cattccttac cttcagcgaa
    16261 ttggtatgga tatccatccc ttcagcggcc ggcattgcgc atgcggcgat cagcgtgcgc
    16321 gctcccttct gttccaccac gcacacacgg cagttgcctg ccacacagag atcggggtga
    16381 ttgcaaaggg tgggaatgat tattccaagc tgtttggcag catcaagtac tgtcgttccc
    16441 tccgctacct ttacttcctg atcatttatt tttagagtta ccattttctt ctttgctagt
    16501 attagtaaat tatctcttcc ctgaaattct caactattga tctgaaggca ttgccaaccg
    16561 actgaccgag gccgcacttg gaagccacct tcatggtatc ggcaagcttg accagttcat
    16621 tcagataggt agatttggct tcacccttct taaccttctc aactcccttg agcagctgct
    16681 ggcatcccac acggcagggt gtgcactgtc cgcatgactc ctccgtgaaa aaatccaggt
    16741 aattgtggag aacgttgtac atcgaacggg aactgttgaa cagtttcatc gaacctcccg
    16801 taggtactcc ctcgaaaccg atgattgtat cctcgaactt cttgcgcggt acacagaagc
    16861 ccgatgctcc tcctacctgc actgccttcg tgtcgccatc gccgaactca ttgacaaact
    16921 cctggacggt catgccgagt tccagctcga agattcccgg aatgggcgtg tcacccgata
    16981 tggaaaagac ctttgatccg cgtgagtcct tggtcccaag cttgacgtac tcctgcggcc
    17041 ctttcgcgat gatcatcatc gcagtcacca gggtctccac gttgttgatt gatgtcggtt
    17101 tggagaacag ccctgatgtg gtcggatagg ggggcttgtt tcgcggctcg ccgcggaatc
    17161 cttcaattga ttcgaacagt gcgctctcct caccgcagat atatgccccg cttcccatgc
    17221 ggtactcaat ggtgaagtcg tacccgatct ctgcaatcat cttctgaaac ttatgcagtt
    17281 cgaggtaaag ctgcggcaga aggaacctgt attcaccgcg aaggtatatg taacctttct
    17341 ttgctcccat ggccttgccg gctatagcca tgccgccgta taccttatat actacacggt
    17401 caaggatctc acggtccttg aaggtgcctg gttctccctc atcggcatta catactatat
    17461 atttctccgg atccttctcg gcagcggtga atttccactt catcccggtc gggaaaccgg
    17521 cacctcccct gcccttcagc cctgattcca gtacctggtt tatgatttct tccggggtca
    17581 tcgagaaagc tttcgccagc acttcttttg catctatgct ctcgaatata agatcaactt
    17641 tggtcagtcc tttgcttttc attgcagtgc ctcctcttta tttacgcatg taacctttta
    17701 taacatctat caccgtctct gacgagagat cggtgtaagg catgtcattg accagcattg
    17761 caggcgcctt gtgacaccat cctatacaat tggtctcaag cagagtaaac atcttgtcag
    17821 gggtggtctg gccaaccttt atcttcagta tttcctccag cgtgctgata acgtcattct
    17881 tccccttcat tgagcatgtg atggtcttgc agaccctgat cacattcttc cccctctctt
    17941 ccgtgtcaag aaaagtatag aacgatgccg ttccgtatac ttctgccgcc gagatgtcaa
    18001 gctcggtggc aacctctacc atcgcttcat cagtaagata attgtgtttt tttacaatgt
    18061 cctgaaggat cggcataagg ctctctctct tccgcccgta tttgtctgcc agctccttca
    18121 ccatgttacc agtttgatac atttctgttt gggttttagt ggttatttct tattgcattt
    18181 gtgttaagat tccaactgtg aaaataataa caataaactt tcaaaaaacc ttacttatgt
    18241 catgtttttt aaaataaatg ttatgcaaca cctctcacgg ccatgcagag caatgctgaa
    18301 gtgcgcatgc tatcccgtca atgatgccaa tttctccacc ccttttacct gaatcgtcca
    18361 ccattttttt gtattttaag ccatctttgt gtttaacaaa acaataaata tgaaaaccaa
    18421 tatcgaagta agatatgcag ctcatcctga tgatgtcagg cattgggaca cagaccggct
    18481 gcgccgggaa cacctggtat cagcggtttt tcaggacaat gccgtgacaa tggtttattc
    18541 tctttatgac cgaatggttg cgggaggcgc caaaccggcc ggcgaagcgc ttaagcttga
    18601 gcctgttgat gaggtgaaag ccgcattctt cctgcacaga cgcgagctgg gggtattcaa
    18661 cgtggggggg cctggcaggg taactgccgg cggagcggaa tacgcactgg aatacaagga
    18721 ggcactgtac ctgggctgcg gtgacaggga agtgactttt gaaagcagtg atgccgcaaa
    18781 tcctgccctc ttctatttca attccgcacc tgcacaccgc aactaccctg accgcaaggt
    18841 tacaaaaaag gattgcatca ccgcagagat gggatccgcc gaagcagcca accacaggat
    18901 catcaacaag atgatagtac gggaagtgct cgaaacatgc cagctgcaga tggggatgac
    18961 cgaactgaaa cccggcagtg tatggaacac catgccgccc catacccacg gccgtcgcat
    19021 ggaggtttac ttttattttg aagtccctga cggcagcgtt gtatgccatt ttatgggcca
    19081 gccgtcagag acaagacata tatggatgaa aaacaacgag gctgtgattt cacctcagtg
    19141 gtcaattcac tcagctacgg caacctcaaa ctacacattc atctggggta tggcaggaga
    19201 aaacctcgat tacggtgacc aggattttct gaagcagact gacctgcggt agatacgcaa
    19261 aaacgaataa gagcagtgac atgaacagca accttttcag acttgacggc cgggtggcgc
    19321 ttgtcaccgg cgcctcatac gggatcggaa tggcgatggc aaaagccctg gcagaagcag
    19381 gagctactct tgtgttcaat gaccggaatc ccgacagggt gactgaagcc ctcgccggat
    19441 acagaaagag tggcatcgat gccaggggat atgttttcga tgttactgac gaaacagctg
    19501 tcagggagta tattgaaaag atcacctccg agatcggcgt gattgacata ctggtaaaca
    19561 acgcagggat cataaaacgg atacctgccc ttgagatgga tgctgctgac ttccgcgagg
    19621 ttatagacat cgaccttgcc ggcccgttca tcatgtcgaa agccgtggct ccgggcatga
    19681 ttaggaaagg gagcggaaag atcataaata tctgctccat gatgagtgag gtgggacgtg
    19741 aaacggtctc ggcatatgcc tccgccaagg gcgggcttaa gatgctgacc cgcaacctcg
    19801 cctgcgagtg ggctgaatac aatatccagg tgaacggtat cggaccagga tatatcgcaa
    19861 caccccagac agcaccgctg cgcaccgaag gccatccctt caactcattc atcatcggcc
    19921 gtacgccggc agcacgctgg ggaacacccg gagacctcca gggaccagtg gtttttctgg
    19981 cctctcctgc ttcagatttc gtaaacgggc acatcctcta tgtagacggc ggaatactcg
    20041 catatattgg aaaacaaccc aggtaaagat cagatactat gcaaaagctc tcactcttcc
    20101 tgatatcact ctttctcctt gccggctgca gcggcaaccg gatgaaggtc acccttgata
    20161 atgccctttc cattgaccgg tttgccgaaa ccgttgagat tcccatcagt gacattgcct
    20221 ccaggttggg tgatattgat ccggcagcac tcgtagtgac cgacagcaaa ggcgctgagg
    20281 tgccgtcaca gatcatccgg gcgggccagg ccgaaccgct ctcgcttatc ttccaggcgg
    20341 atgtaaaagc aggctcttcc gcaaggtact acatcgcttc ggggactccg cagagttatg
    20401 aaccaaaggc ttatggccgg ttcgtaccgg agaggtacga tgactacgcc tgggaaaatg
    20461 accgtatcgc cttccgcatc tacggaccgg cgctcatcgc caaggatggc ccgagcaacg
    20521 gcctcgacgt atggataaag aggaccggca aaatggtcat agacagatgg tattccgact
    20581 acctcgcggg cataaacact taccatgacg ataacgggga agggtgtgac tgctacaagg
    20641 tgggacgcac actcggcgcc ggggccatgg caccgtatgt tgatgactca ctctggctgg
    20701 caatcaactt cgaaaactgg aagacccttg acaacggacc cgtaagaacc tcgttccggc
    20761 tcacttaccc acctttcctg gttcgtgacg tcatggtgac cgagacacgg acaatctcac
    20821 ttgatgcagg cagccagctg accaaaataa ctgaagaata cacggggatt gacaaggaat
    20881 ttaccgtggc agcagggata gtcaggaggg aggaaggctc tcccctgctc tttcctcctg
    20941 agaagaatgc aatcgcctac cgccttgacg gtggtgaggg cgggataaca tacattggca
    21001 ccgtgctgac cacacccgcc acagcagtgg aggatatcgc tgatcacctt atgattgtca
    21061 cacactaccg cccggggaca cccctgacct actacactgg ggccggatgg agcaagtggg
    21121 gatttgaaac agatgctgcc tggacccgct atgtaaacga gttctctcag aaactggctt
    21181 ctcccctgaa ggtcaaaaca gaataacagg atatgcggtc cgctgcctgt aaaacggccc
    21241 tggtgctgct gttgatggtg acggcggcac ttcgcacagg ctgcggtcag tttaagagtg
    21301 cattcaccca ttattcagtt gaggacggcc tctcagaggg agttgttctc accatgcacc
    21361 aggaccgcga agggcttatg tggttcggaa cgttcgacgg ccttaaccgg ttcgacggat
    21421 acaactttaa ggtttacaaa tcgggataca actccgcata cggacttaca aacaaccgcg
    21481 tggaccggat caccgaagat gacctgggct atctctggat catgaataat gacggtgagg
    21541 tgtaccgtat gaacacccgt tccgaggagt tcatcaactt caatgagata agctcctcgg
    21601 gatcaggcaa ctactccccc gtcaaccaga tctgctgttt cagcggcgga gagacctggc
    21661 tgcaggccga agccggaaca tgcatcaggg ttatctcatc ggcttcatct ccaggttaca
    21721 cgatagacag gttcaattgc cagggcagtg ataacactcc ggtaaaggtc aacctcatca
    21781 gcagggacag tcgcgggacg gcctggatac ttacctcagt cggactgttc cgggtcaggg
    21841 gagatgataa agtgcccgtc agggcactca ccccgggcat tcgcccggag ggcagaaacg
    21901 gtttcttctg cattcatgag gcgggagaca ggttatggtt cggcaccggc aacggaacca
    21961 tcattattac cgatgctgag gccggtgcgt acgaggaact tagcctggga gtgcgctccg
    22021 acattatcag catcaggcag ctgaaccagc gtgatctgct gatccttacc tccggcgacg
    22081 gcttcttcgt ctattcgccg gttacggcag aaaaaaagca attcgacacg tctcggtacc
    22141 cggcgatgcc gacaaacagc atggtatcgg catacgtgga caggtatggc gaagcgtgga
    22201 tcgaatctgc tgccgccggt gtggtgcact atgatccgtt cacgggaacg gtgcggcact
    22261 tcaggatgaa tgttgacaag acaaacccga atgtgctcct tcccagtttc gtcatatttg
    22321 aagatgtcaa tgatatgctc tggatctatc ccaggggagg aggattctcg cgttataaca
    22381 gggtagagcg caggcttgaa tactttttca atgaaccggg aggtgctgac agacggtttc
    22441 ccaacctgat acattcggcc ctgtcagaca accaggggaa cctgtggatg tgcaccatca
    22501 cgcgagggat agaaaaagtc tctttcttcc cgaacctgtt cagggtgctg agccccaaac
    22561 ctgtggcagc cagccactca gaaaacgaag tcagggctat cttcgaggat aacaaccgcc
    22621 atttgtgggc cgcaacgcga gacggcaggg tttacctgtt cgatcctgca aaaaaaatgg
    22681 tgggatacct cagggacaac ggcaccttca gctcgggcga agcattcaat gccctggtgt
    22741 acgtgatcac ccaggacagc agggggagca tatggatagg atcaaaaggc ggaggcctct
    22801 tcaggcttga gcctgtgacg aaagggcccg cttactccta caggatcaca ggataccgcc
    22861 atgacgatgc agacccgtac agcctgagtc atgacaatgt ctactctgtg catgaagacc
    22921 gcggcggacg gatatgggtg ggcacattca acggcggtat aaactacatc tcagaggaag
    22981 gcggcagggt aaggtttatc aacgcaggca acagacttaa gaattacccc tattcaaacc
    23041 ataaaagggt aaggtatatc acctctgaca accatggaag gatttgggtt ggcactacca
    23101 gcggcctggt gattttcagc agggacttcg cggaacctga agacgcagag ttcaccacct
    23161 gcttctttaa tcctgatgtc gagaccagcc tggccaacaa cgatgtccat tacatatact
    23221 gctcgcccga tgacgaaatc tttgtagcca cattcggcgg aggcctnnnn nnnnnnnnnn
    23281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23341 nnnnnnnnnn nnnnnnnnnn nnnnnnctca ggacaagtga cagcgagatc atgttcggat
    23401 ttgacaacgg catctactgc tttaatccgg cacaggtcag gaaggtgaat tatgttcccc
    23461 cgcttcagtt cacacgcctt ctggttgccg gcaccgatgt acataccacc ggcggcaact
    23521 acaaccggcc gggtgaaaag ggtgagcagg ccacgcttga gctgaagcat gaccagaaga
    23581 gtctcacaat tgagtttgcg gcactcgatt accggaatcc aaaaaacatc cagtacgccc
    23641 acaggctcga gggggttgat cctgactgga taatcgcaag agggcagcgt accgcctcat
    23701 ataccaatct tgatcccggt gaataccttt tcagcgtaag gtcaaccaac tccgacggca
    23761 tctgggttga caacacacgc acggtcaggg ttacggtgaa accctcattc tggcagacac
    23821 tctttgccag gctactgatc gtactggccg gtgctgcact gatagcgctt gcaatttatg
    23881 tcatcttcac catgtacaag cttcgtcaca aggtggaggt tgaacagatc atcaccaacc
    23941 tgaagctgaa gttcttcacg gatatttctc acgagctccg tacccctctc acactcatct
    24001 cctcgcctgt aaccaacatc ctgcagaaca accggctcga gcccgaggta agagatcagc
    24061 tgaccctcgt gcagaacaat acccagcgaa tgctgaggct cgtaaaccag atacttgact
    24121 tcaggaaagt acagaaccgg aagatgaagc tgagaataga ggagttcccg ctcggaccgt
    24181 ttattaaggg catatgcgct gacttccgca aggcggcaga ggataagggg atcaggttta
    24241 cagttgatga cctttctgaa ggtgaaaagg tatgggctga ccgagacaaa gtggaaaaaa
    24301 tcgtctttaa cctgatcacc aacgcactga agttcacccc ccgcggaaag aatgtgactg
    24361 tcactgtcag aaagaacagt gactctctgg agctgatagt ggcagaccag gggaccgggc
    24421 tctccaggga gaagataaaa aacctcttca tgcgtttcag cagtgatgaa ggggaggtga
    24481 caaccctcca gcccggcacc gggataggac tgtcgctcag cagggaactg gcggaactgc
    24541 accgtgccga actgactgcc gagagtgagg agggcaaggg agccaccttc cgggtaattt
    24601 tcagacttgg attctcccac tttgacaacg aaacggaatt tgtcctgtct gacggacaga
    24661 atcctctcag aacacgtcag acgccttcag ccatgcagga agaagatgag gagaatgagg
    24721 ccgtggaagg cgatgatgaa cccgttctgc tgttcatcga agacaacgaa gagctccgga
    24781 gcttcataaa gattatcctt tctgacggat tccatgtcat tgaggccggc aacggtatcg
    24841 aagggtttga gattgcacgg aaccagcttc ctgatatcat tatcactgac ctgatgatgc
    24901 ctgagatgga cgggcttgaa ctggcacggc ggataaagga agaggtcacc acttcacata
    24961 tcccggttgt tgtccttaca gccaagtcag accttgacac gcaggtcgaa gccctgaaac
    25021 ggggagctga cgatttcatc accaagccct tcagctccac ctacctaagg gccaggatcg
    25081 aaaacatact ccggcagcga aaaaaactgc aggagctgtt cctctcctca cttgatgact
    25141 atcaccggcc gaaaaacggt aaacctgttg atatcactcc gtcggaaccg gtaatagaat
    25201 cgtatgatga caggctcatg aaaaatctga tggaggtaat ggaggagaat attgacaact
    25261 cggcgctcac cgttgatgag cttgtgtcga aaatgggcat cggcaggtct gtcttcttca
    25321 aaaagctgaa gagcctcaca ggcctggccc cggttgagtt tatcagggaa gtgagggtca
    25381 aaagggccgc tcagctcata gaatcaggcg aatacactat ctcccaggtc acctacatgg
    25441 ttggctgcaa tgatccccgg tatttctcgc gcatcttcaa acaacgcttc gcaatgactc
    25501 ccacagaata cagggaca
//
LOCUS       DAOPXU010000033        24842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_2005_length_24842_cov_9.629685, whole genome shotgun sequence.
ACCESSION   DAOPXU010000033 DAOPXU010000000
VERSION     DAOPXU010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24842
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2005_length_24842_cov_9.629685"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1735
                     /locus_tag="PKK03_05010"
     CDS             <1..>1735
                     /locus_tag="PKK03_05010"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNX83789.1"
                     /translation="MKIDQEQVQLMKRRGGVGHDLSHIRPKGSPVLNSALTSTGVVPF
                     MERYSNSTREVAQDGRRGALMLSISIKHPDAESFIDAKLEAGKVTGANVSVKITNDFM
                     QAVIDGKSFRQQYPIKSENPKYVKDIDARKLWNKIVHNAWKSAEPGVLFWDTIRNESV
                     PDCYADLGYETVSTNPCGEIPLCPYDSCRLIAINLFSYVKDPFTPKAKFDHDLFRQHV
                     HLSQRIMDDIIDLEQEKIDAIIAKIEADPEDDELKRVERKMWENIRHKTGEGRRTGIG
                     ITAEGDMLAALGLRYGTDEATEFSVNVHRTLAIEAYRASTDMAAERGAFPIYSAEREK
                     NNPFISRMRETDPEMVAEMEKTGRRNIALLTIAPTGSTSLMSQTTSGIEPVFMPFYKR
                     RRKVNPNDKDVKVTFIDEVGDHWEEFTVFHHKFIDWMKVNGYNYEEVKDMSETQINDI
                     VAVSPYYKATANDVDWVAKVRMQGAIQKWVDHSISVTINLPAEVKEELVSELYLTAWK
                     EGCKGATVYRDGSRAGVLVSNSKEEKKSDWSPVRPKVLDADVIRFQNNEEKWIAFVGI
                     KDGVPYEIFTGL"
     assembly_gap    1736..1835
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2102..3001)
                     /locus_tag="PKK03_05015"
     CDS             complement(2102..3001)
                     /locus_tag="PKK03_05015"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNX83790.1"
                     /translation="MFRNHIHKPTVLVTLAALTVVLSCRKDPPVPDDTSIFAACVIPG
                     TESTIDIMSFNVQGFPKAGYASIAALADLIMAMDPDVVALQEVASEADFNRLVDLMPG
                     WSGAYYPVNNDEWNLAYLIRVSEQETINGSLRTLFEGDSYAFPRPPLEIKVRHRKSGR
                     EITLINLHLKCCEGQDNESRRRSASEKLKEYLDQQRPDDAVVMLGDYNDGIASASGTE
                     NPFLNFINDDSSYRFADMAIALGSGLWWSYPSWPSHIDHIMVTDELFGSIDTTMVIKA
                     SPCYPEYEEVISDHRPLMIAITP"
     gene            complement(3181..4548)
                     /locus_tag="PKK03_05020"
     CDS             complement(3181..4548)
                     /locus_tag="PKK03_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNX83791.1"
                     /translation="MEKITKILLTEKEMPRQWYNIMADMPNKPLPPLNPATGKPVGPE
                     DLSVIFPMELIKQEVSTERYIAIPDEVVDLYYTYRPSPLFRARSLEKALGTKSRIYYK
                     YEGGNYSGSHKANTAIPQAYYNKQEGVRRITTETGAGQWGSAMSFACNHFGLELLVFM
                     VRVSYNQKPGRKLMMNTYNARVVPSPSTMTDAGRKVLEKDPDSPGSLGIAISEAMEIA
                     AQDPYTKYSLGSVLNHVILHQTIIGQEAMIQLEKAGDYPDVVIGCFGGGSNFSGIAFP
                     FLRENFVNGKKTRLVAVEPASCPKLTKGKFTYDFGDQSGMTPLLPMYTLGHNFIPDKI
                     HAGGLRYHGAGVLVSQLRKDGFVEAKAKFQRECFEAAILFARAEGILPAPESSYAIAG
                     ALDEARRADLEGVSRTILFNLSGHGHFDITAYEKYFEGNLPDHEATPDEISQSLLNSA
                     VPVTI"
     gene            complement(4792..5370)
                     /gene="hisIE"
                     /locus_tag="PKK03_05025"
     CDS             complement(4792..5370)
                     /gene="hisIE"
                     /locus_tag="PKK03_05025"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HNX83792.1"
                     /translation="MDFNKGDRLVPVVIQDNTTLQVLMAGYMNEEAYRKTEDEGLVTF
                     YSRSRQSLWTKGETSGNYLRVVTIETDCDSDTILIKAEPAGPVCHTGTWSCFGEQSAK
                     GFIYRLEDIINSRIEEDSSGSYTSRLFNEGTGKIAQKVGEEAVELVIEAMGDNIGLFK
                     NEAADLLYHLLILLRAKQVNLSEIEEVLLSRH"
     gene            complement(5373..6128)
                     /gene="hisF"
                     /locus_tag="PKK03_05030"
     CDS             complement(5373..6128)
                     /gene="hisF"
                     /locus_tag="PKK03_05030"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNX83793.1"
                     /translation="MLKKRIIPCLDIKDGRTVKGISFVNLRDAGDPVELARAYAEQSA
                     DELVFLDISATDERRKTLAPLVKKIAEAINIPFTVGGGITTREDVSMLIRSGADKVSV
                     NTAAILNPPLIAELADEYGSQCVVVAIDAKLVDGTWVVCTHGGKRVTTLPAITWARES
                     ERLGAGEILLTSVNRDGSGEGFSLDITSAVCNCVNIPVIASGGAGSPEHFREVFAETG
                     CTAALAATLFHYGIIKIPELKKYLLHNNIDIRC"
     gene            complement(6122..6835)
                     /gene="hisA"
                     /locus_tag="PKK03_05035"
     CDS             complement(6122..6835)
                     /gene="hisA"
                     /locus_tag="PKK03_05035"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNX83794.1"
                     /translation="MRIIAAIDIIDGQCVRLTQGDFGRKTLYRADPVDLAREMEDNGI
                     RYLHLVDLDGARTGRITNQRVLEGIASATGLEIDFSGGIRTEEDILLAFSSGAAQVTI
                     GSIAVHRPDLLRQWLGQYGPEKIILGADFRERKVATGGWQETSGRDIADFLREYRLAG
                     IRYALCTDISKDGMLTGVSAEIYRELTSLEGLAVIASGGITAVSDLRELRDAGCEGAV
                     TGKALLEGRITYNEIAEIC"
     gene            complement(6832..7416)
                     /gene="hisH"
                     /locus_tag="PKK03_05040"
     CDS             complement(6832..7416)
                     /gene="hisH"
                     /locus_tag="PKK03_05040"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNX83795.1"
                     /translation="MMIAILKYNSGNTMSVQVALRRLGHDSVVTSDPEILLKSDRVII
                     PGVGEARSAMSHLRERDLDSLILPLKQPVLGICLGLQLMCSRSEENNTECLGIFDAVV
                     RRFPPEERVPHIGWNNFESLSSSPLLKGLEPDDDLYYVHSYYAELNSDTKAVCRYILP
                     FSAAMQRDNFHAVQFHPEKSAEAGDRLLINFLEL"
     gene            complement(7410..8498)
                     /gene="hisB"
                     /locus_tag="PKK03_05045"
     CDS             complement(7410..8498)
                     /gene="hisB"
                     /locus_tag="PKK03_05045"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HNX83796.1"
                     /translation="MKKVLFIDRDGTILKEPEDEQVDSLEKLEFMPGVIGALSAISRE
                     TGFELVMVTNQDGLGTPAYPEEAFLPPQNKMLSILAGEGVRFAEIFIDRSFESDRTPT
                     RKPGTAMLTRYLADGVDLGSSFVIGDRLTDIELAKNLGCRVIYVSDRQNGEASLCTRD
                     WQEIRRFLTGIPRRASVSRETNETGISIEIDLDGTGKGSISTGIGFFDHMLEQVARHA
                     GIDITLKASGDLHVDEHHLVEDTAIVLGQALDRALGKRKGMARYGFALPMDDSAAEVL
                     IDLGGRPWLEWNASFRSDRAGTLSTDMFRHFFRSLSDNAKCNIHVRATGDNDHHKAEA
                     IFKAFARALGMAVVKTGSGNLPSTKGTL"
     gene            complement(8495..9526)
                     /gene="hisC"
                     /locus_tag="PKK03_05050"
     CDS             complement(8495..9526)
                     /gene="hisC"
                     /locus_tag="PKK03_05050"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNX83797.1"
                     /translation="MKRTDKLVRPNVLKLTPYLCARDEYEGSEGIFLDANESPYGPLN
                     RYPDPRQRELRETISRKKGIPAENIFLGNGSDEIIDLCFRIFCNPGKDRAMIFTPTYG
                     MYGVAASVNDIGIVRIPLDDTFNIDLQAAKPLLREDTLKLVFICSPNNPTGNCMNPEK
                     VETIIRELNGIVVIDEAYADFSGKPSFAERIGEFDNLIVMQTFSKAMGLAAARVGVAF
                     ADADLLRYFFAMKPPYNISTINQEAALLRLNEADAVARRVQSIITEREKLASRLTALP
                     VVEKVWPSDSNFLLVRVKDADYIYNTLAERKIIVRNRNREIPGCIRITTGTPEENEKL
                     ISELESLSI"
     gene            complement(9632..11017)
                     /gene="hisD"
                     /locus_tag="PKK03_05055"
     CDS             complement(9632..11017)
                     /gene="hisD"
                     /locus_tag="PKK03_05055"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNX83798.1"
                     /translation="MRILLYPGAAELAAALARPSDGNREIDMKVREILSAVMNGGDAA
                     LRKYSEKLDGFAAASLRVEADEIEQAECEVSPALKKAILSAASNIEKFHLAGAGTARI
                     TRTAPEAKRTTPEVTGSTPEVTPTAPEIIETAPGVRCWYRDLPIEKVGLYIPGGTAPL
                     FSTVLMLGIPARLAGCGEIVMCTPPGRDGKINPLILYAAKVAGVKAVFRAGGAQAIAA
                     MAYGTESIPAVYKIFGPGNRFVTRAKEIVQLEGSAIDMPAGPSELMVIAGSNANPAFI
                     AADLLSQAEHGTDSQVIMLTYSREMITLVEKETERQLALLPRKDIAARSLGNSSLVLL
                     ESEEKCLEAANLYAPEHLIIATPDPIRTSERVTSAGSVFLGNYSSESPGDYMTGPNHT
                     LPTGGFARSFSGVTIGSFMKRIFFQEVTAEGIRNIGPDTVLMAEAERLGAHAAAVSVR
                     LDYLDKNTQKK"
     gene            complement(11017..11868)
                     /gene="hisG"
                     /locus_tag="PKK03_05060"
     CDS             complement(11017..11868)
                     /gene="hisG"
                     /locus_tag="PKK03_05060"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNX83799.1"
                     /translation="MLKIAIQKSGRLSESSRKLFDECGIRISNGAGTLKVVAGNFPAE
                     ILLLRDDDIPQYVEQQVADIGIVGENIVLEKQREVDILEQLGFGGCRLSLAIPKEDNY
                     TGPGYFMNKRVATSYPSLLTGYFRSRNIRAEIEELSGSVEIAPGIGLADGICDIVSSG
                     STLLTNGLKEVETVMKSQAVIIANRNISGQKREILEKLLFRIRAVKNASENKYILLNA
                     PEAAVPEICRILPGMKSPTILPLAEKGWCSLHSVVREDEFWERIDRLRSAGAEGILVI
                     PIEKMIV"
     gene            complement(12152..13225)
                     /gene="leuB"
                     /locus_tag="PKK03_05065"
     CDS             complement(12152..13225)
                     /gene="leuB"
                     /locus_tag="PKK03_05065"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNX83800.1"
                     /translation="MNYKIALLPGDGIGPEVIEQAVRVLNVTGEVTGHTFSFTEGMVG
                     AVAIDHTGNPFPDETLHLCRRSDAILFGAIGHPRYDNDPSAKVRPEQGLLRMRSELGL
                     YANVRPVKSIPGMAGISPLKEEIVAGTDIVMIRELTGGIYFGKPRGRSEDGLTAFDTC
                     TYSRYEVERVVHLGCRYAVQRRKKVTVVDKANVLATSRLWRETAKEIVAEYPEIEMEF
                     MFVDNAAMQIIRNPSAFDVIVTENMFGDILSDEASVITGSLGMLPSASIGLSASLYEP
                     IHGSFPQAAGKNTANPVGTILSAAMMLELSFGLVKEAGLITRAVEETIREGVVTPDLS
                     HDNPVTTTAMGQRICEQVKALSR"
     gene            complement(13215..14777)
                     /locus_tag="PKK03_05070"
     CDS             complement(13215..14777)
                     /locus_tag="PKK03_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNX83801.1"
                     /translation="MKVEILDTTLRDGEQTKGVSYSPHEKLHIATMLLRDLKTDRIEV
                     ASARVSPGEQEAVRLITSWAEQNGMADRIEVLGFVDGARSLDWIREAGGRVINLLCKG
                     SSRHLEKQLRKTPDEHLCDILDIVSMAETRDMIVNIYLEDWSNGMLSSPGYVIRMVDA
                     LAGSGIKRIMLPDTLGILNPDQTFNFCSQMTARQPEVHFDFHPHNDYDLSVANIFSAV
                     KAGVRGVHTTVNGLGERAGNAPLSSVIALLRDHLDAETDVNEYEITRISRMVEKFSGI
                     RIPVNKPVIGANVFTQTSGVHADGDRKDNLYCSTLGPERFGRKREYALGKQSGKATIR
                     KNLEEFGLELSPDLIARVTERVIELGDKKESVTRDDLPFIIADVLGDDRSGYRVFIRN
                     YSLNLTCGLKPSASVQVEIDGILHQETAAGDGQYDAFMKALRKIYESLGTGLPLLTDY
                     EVSIPPGGKTDALVETVIRWNHNGREFRTRGLDADQTESAIKATEKMLNIICNNETNK
                     EYNKLIEIVNEL"
     gene            complement(14774..15370)
                     /gene="leuD"
                     /locus_tag="PKK03_05075"
     CDS             complement(14774..15370)
                     /gene="leuD"
                     /locus_tag="PKK03_05075"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNX83802.1"
                     /translation="MKRAEFTILESTAVPLPLANIDTDQIIPARFLSATGREGFGNNL
                     FRDWRYDADGNPVPGFVLNRPEFRGEILIAGSNFGCGSSREHAVWAITGHDFRAVVSD
                     SFADIFMNNALNNGLLTVTVTPEFLAQLTGLITSKPETIVRVDLPGQVITVPGSGLQA
                     GFPVNPFRKECLIKGLDNIDYLLSMKEDIMAFEKNRQA"
     gene            complement(15482..16891)
                     /gene="leuC"
                     /locus_tag="PKK03_05080"
     CDS             complement(15482..16891)
                     /gene="leuC"
                     /locus_tag="PKK03_05080"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNX83803.1"
                     /translation="MSGKTLFDKIWDAHVVRSISGGPDVLYIDRHYIHEVTSPQAFNG
                     LRQRGINIFRPERIIATSDHNIPTVNQHLPIADPVSRLQVETLEENCRAAGIRYYGMG
                     DPRNGIVHVTGPEQGFTLPGMTIVCGDSHTSTHGAFGTIAFGIGTSEVEMVLATQCLL
                     QTRPKQMRINVKGELHEGVTSKDLILHIIASLTAGGATGYFVEYAGEAVRKLSMESRM
                     TVCNMSIEMGARGGLIAPDEVTFNWLAQIPSVSDNREFARLIHHWHSLRSDRGAGFER
                     EVTFDASKVTPMITYGTSPGMGMAVNGVIPSPDYAGSSANGSYLRSLEYMGFKPGMKL
                     LGHPIDYVFLGSCTNGRIEDLRLFTAAVAGKKKAAGVTALIVPGSQQVARQAEREGLT
                     ETLRLAGFEMRQPGCSSCLAMNEDKIPSGKYAVSTSNRNFEGRQGTGARTLLAGPLVA
                     AAAAVTGKITDPRELVWNQ"
     gene            complement(16869..18386)
                     /locus_tag="PKK03_05085"
     CDS             complement(16869..18386)
                     /locus_tag="PKK03_05085"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNX83804.1"
                     /translation="MDKNRVIFFDTTLRDGEQVPGCQLNTVEKIEVAKSLEALGVDVI
                     EAGFPVSSPGDFRSVVEISKAVREPVICALTRAVKKDIEVAAEALQYAERKRIHTGIG
                     TSPWHIQYKIRTTPEEIIKRAADAVRYARKFTDDVEFYAEDAGRSDNEYLARVTEAVI
                     NAGATVVNIPDTTGYCLPEEYGSKIRYLMENVPNIDRAILSTHCHNDLGMATANTMMG
                     LINGARQAEVTINGIGERAGNTSLEEVAMIIKSHHDLNLTTAIRSKLIFSTSRLVSTL
                     MSMPVQPNKAIVGRNAFAHSSGIHQDGVLKKRENYEIIDPNEVGVRESSIILTARSGR
                     AALNHRLEALGIELGKEELDEIYHRFLMLADKKKEINDEDIRILSGEVFRGEKRLKLE
                     SLQVLCGNSVMPVATVGIRVNGEVHTSTAPGNGPIDAAFTAVKHLIQENVHLEEFLIQ
                     AITRGSDDMGKVHIQVEHEGNIFYGFSANTDIVTASVEAFIDAISKIDNERKDTL"
     gene            complement(18403..19452)
                     /gene="ilvC"
                     /locus_tag="PKK03_05090"
     CDS             complement(18403..19452)
                     /gene="ilvC"
                     /locus_tag="PKK03_05090"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNX83805.1"
                     /translation="MAQIDFGGVIENVVTRQEFPLSKAHEALKDEVVAVIGYGVQGPA
                     QAMNMRDNGINVIVGQAPEFRADWDRAVADGFIPGRTLFSVEEAASRGTIIQYLVSDA
                     AQRILWPVISPHLTAGKALYFSHGFSVTYKQHTGVIPPPDIDVILCAPKGSGTSVRRN
                     FLAGAGINSSFSVFQDHTGRAGERVIALGIAIGSGYLFPTTFEKEVFSDLTGERGVLM
                     GALEGIMEAQYQVLRENGHSPSEAFNETVEELTQSLIRLVDEKGMDWMYANCSATAQR
                     GALDWKPRFREATLPVFRELYEKVSSGEECVRVLTSTGAPGYREQLNAELAEMGSSEL
                     WKAGAAVRKLRPDGK"
     gene            complement(19482..20042)
                     /gene="ilvN"
                     /locus_tag="PKK03_05095"
     CDS             complement(19482..20042)
                     /gene="ilvN"
                     /locus_tag="PKK03_05095"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNX83806.1"
                     /translation="MKQEFTISLFSESHIGILSRITLILSRRQINIESITASESAVPG
                     VQMLTMVVRTTPEMVQKVARQVEKVVDVLKVFVHTSSEIICQEMALYKVTTRGLMSGS
                     MISDIVRKHNARILEVKSEYMVVEKSGTRAEIKELLALLEPCGVLQFVSSGRVAVTRQ
                     VKELNAYLREMEEARGTRQEALEFYN"
     gene            complement(20039..21760)
                     /gene="ilvB"
                     /locus_tag="PKK03_05100"
     CDS             complement(20039..21760)
                     /gene="ilvB"
                     /locus_tag="PKK03_05100"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNX83807.1"
                     /translation="MTEDNNHKAITGAEALLKSLVEEGVDTIYGYPGGAIMPVYDRLL
                     DYTGSLRHILTRHEQGAAHAAQAYAMVAGKPGVCFATSGPGATNLVTGIANAFLDSVP
                     VVFITAQVASPLIGTDAFQETDILGISMPVTKWNAQVKKAAEIPDTIARAFYIARTGR
                     PGPVLVDISKDAQIETMNFRYRRCTYIRSYNPVNPIVNRDIESAAVMINHSERPLILA
                     GHGVIISGAVAELKSLAEKGGIPVAVTLLGIPAFSHDHRLFAGMLGMHGNYAPNVLTN
                     RADLIIAAGMRFDDRVTGNLKKYAPQARVIHIEIDSSEIDKNVRADLGINGDVKEVLT
                     AIFPHIRERKREEWIREFRILDSTEHEKVIRPETEPENGELKMGEVVKVISEHAGRDA
                     VVVTDVGQHQMAVARYYHFTDSNTLVTSGGMGTMGFGLPAAVGAATGTSDRQVIAFIG
                     DGGIQMTLQELGTIMQYHIPVKIVLLNNSFLGMVRQWQEMFFNSRYASTEMVNPDFVA
                     LASAYGIPRRKIKNREELKASAAEMLDSDGPFILVAEVSRTANILPMVEPGASVSEVT
                     LTYNSKS"
     gene            complement(21773..23599)
                     /gene="ilvD"
                     /locus_tag="PKK03_05105"
     CDS             complement(21773..23599)
                     /gene="ilvD"
                     /locus_tag="PKK03_05105"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNX83808.1"
                     /translation="MKKELRSLQTMSGRTMAGARSLWRANGMKEEHFGRPVIAVVNSF
                     SQFVPGHVHLQQAGSVIREELARRGLFAAEFNTIAIDDGIAMGHGGMLYSLPSRDLIA
                     DSVEYVCNAHRADAMICVSNCDKITPGMLMATMRLNIPAVFVSGGPMEAGVLGGRAVD
                     LIDTMVAAADDTVSGEKLLELEKSSCPSCGSCSGMFTANSMNCLTEALGLALPGNGTV
                     PATHVDRMKMFRRGAQLIADLAERYYIDGDETVLPRSIATREAFMNAMTVDIAMGGST
                     NTVLHLLAIAREAGVDFTMKEIDALSRRVPNICRVSPASGYHVEDVNRAGGVFAIMGE
                     LNRAGLLDASVRRIDCPSLDSALAGWDILSPVVLPEAHDLFLSSPDRSAGPRPFASEA
                     RYGSHDTDRVTGAIRDAGHAYSNDGGLAVLYGNLAPDGCMVKTAAIDSSMMRFSGPAV
                     VFDSQEDAVAGILGGRVREGDVVVIRYEGPRGGPGMQEMLYPTSYLRSKGLDRRCALI
                     TDGRFSGGSAGLSAGHISPEAAAGGTIALVCSGDRIMIDIPARTIRLAVDDTVLETRR
                     RAERARGAAAFTPENRVRTVSGALKVYAAHVSSADRGAIREI"
     gene            complement(23868..>24842)
                     /gene="kbl"
                     /locus_tag="PKK03_05110"
     CDS             complement(23868..>24842)
                     /gene="kbl"
                     /locus_tag="PKK03_05110"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNX83809.1"
                     /translation="GYGMSSVRFICGTGDMHKELEKKIAEFFGTEDTILYAACFDANG
                     GVFEPLLTSDDAIISDSLNHASIIDGVRLCKANRYRYENADMEDLEAQLQLAQQHRFR
                     LIVTDGVFSMDGNVAPLGRICELANKYNAMVMVDESHSAGVVGETGHGVTELYNLRGK
                     IEIITGTLGKAFGGAIGGFTTGKKEIIEMLRQRSRPYLFSNSIPPLVAAAGLKMIEMM
                     TETNELQDKLHHNTRYFMEKMLKAGFDIKPTQSAICAVMLYDAKLSQEMASRLLKEGI
                     YVIGFYYPVVPKDQARIRVQLSAAHETEHLDKAINAFIKVGKDLGVLK"
BASE COUNT         5297 a         6829 c         6323 g         6293 t
ORIGIN      
        1 tcatgaagat tgaccaggag caggttcagc tgatgaagcg ccgcggcggc gtgggtcatg
       61 acctctcgca catacgcccg aaaggctcgc cagtgcttaa cagcgccctc acctctacag
      121 gcgtggtacc cttcatggag cgatattcaa actccacccg cgaagtggcc caggacggcc
      181 gccgtggcgc gctgatgctc tccatctcaa tcaagcaccc tgatgccgaa agcttcatcg
      241 atgccaagct cgaggccgga aaagtcaccg gtgcaaacgt ctccgtgaag atcaccaatg
      301 acttcatgca ggcggtgatt gacggaaaga gcttccgcca gcaatacccg atcaaatcgg
      361 agaacccgaa gtatgtcaag gatatagatg cccgcaagct ctggaacaag attgtccata
      421 atgcctggaa atcagccgaa ccgggcgtgc tgttctggga taccatcagg aacgaaagcg
      481 tacctgactg ctatgctgac cttggttatg agactgtttc aaccaaccca tgcggagaga
      541 tacctctctg cccgtatgac agctgcaggc ttattgccat taatctcttc agctatgtga
      601 aggatccctt cacaccgaag gcaaaattcg accacgatct cttccgccag catgtgcatc
      661 tctcacaacg cattatggat gatatcatcg acctcgagca ggagaaaatt gacgccatca
      721 tagcgaagat agaagctgat ccggaggacg atgagctcaa gagggtggaa cgaaaaatgt
      781 gggaaaacat caggcacaag accggcgaag gcaggcgtac cggcatcggc atcacggccg
      841 agggcgacat gcttgccgca ctgggtctcc gttacgggac tgatgaagca actgagttct
      901 cggtgaatgt acaccgcacc ctggcaatcg aggcatacag ggcatcaacc gatatggccg
      961 ctgaaagagg ggcattcccc atctacagcg ccgaaaggga aaagaataat cccttcatca
     1021 gccgcatgcg cgagacagac ccggagatgg ttgccgaaat ggaaaaaaca ggcaggcgca
     1081 acatcgccct gctgaccatt gccccgactg gttcaaccag ccttatgagc cagacaacct
     1141 ctggtattga acctgtgttc atgcccttct acaagcgccg ccgcaaggtc aacccgaacg
     1201 acaaggacgt gaaggtgacc ttcatcgacg aggtgggtga ccactgggag gagttcaccg
     1261 tcttccacca caagttcatc gactggatga aggtgaacgg ctacaactat gaggaggtga
     1321 aggatatgtc agaaacacag ataaatgaca ttgtcgccgt ttctccctac tataaggcaa
     1381 cagcaaatga cgtcgactgg gttgcaaagg tgcggatgca gggagccata cagaaatggg
     1441 ttgaccactc aataagcgtc accatcaacc ttcccgccga ggtaaaggaa gaactggtat
     1501 ccgagcttta tctgaccgcc tggaaggagg gttgcaaggg agccaccgtc taccgtgacg
     1561 gatccagggc cggtgtgctt gtctccaaca gcaaggagga gaagaagagc gactggtcgc
     1621 ccgtaagacc caaggtgctc gatgctgatg tcatccgctt ccagaacaac gaggaaaaat
     1681 ggatagcatt tgtgggcatc aaggacggcg taccctacga aattttcacc ggtctnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntggaa gaacggggtt gagcgttcgc
     1861 tcaagcgtta tatcccgaat ggcacgaagg caaagggcaa gtgcgaaaac tgcggctcaa
     1921 cctcacttgt ctaccaggag ggttgcctga tatgcactga ctgcggaaca tcaaagtgca
     1981 gctgatcatt tattaaactc catatcacat tgaaaaagag ggtgtctcat aatacaaatg
     2041 ggcacccttt ttctttcata atgtggtcaa aaatagttga tgattttttt aagcagtttt
     2101 ttcagggagt aattgcaatc atcagtggcc ggtggtcaga gatgacctct tcatattccg
     2161 ggtaacaggg actggccttt ataaccatgg tggtatcgat ggatccgaac agttcatcag
     2221 ttaccattat atggtcgata tggcttggcc acgaagggta agaccaccat aatccgcttc
     2281 ccagggcgat tgccatatcg gcgaacctgt atgatgaatc atcattgatg aagttaagga
     2341 aagggttttc cgttccggag gcggatgcga tcccatcatt gtaatcgccc agcattacca
     2401 cggcgtcatc gggtctttgc tggtcgaggt attccttcag cttttccgat gccgagcggc
     2461 ggcggctctc attatcctgg ccctcgcagc acttcaggtg gaggtttata agggtaattt
     2521 cccttcctga tttcctgtgc ctgactttta tctccagcgg aggacggggg aaggcataac
     2581 tgtcaccttc gaaaagggtc cgcagagagc cgtttattgt ttcctgttca ctgaccctga
     2641 tcaggtatgc caggttccat tcgtcattgt ttaccgggta gtaggctccg ctccagcccg
     2701 gcatcaggtc gacgagcctg ttgaaatcgg cttcggaggc cacctcctgg agtgccacca
     2761 catcggggtc catggccatt atcaggtctg ccagtgctgc gatgcttgca tatcctgctt
     2821 tcggaaatcc ttgaacgttg aaggacatga tgtcaatggt gctttcggtg cctggaataa
     2881 cgcaggcggc aaagatgctg gtgtcatcgg gtaccggagg atctttgcgg caggaaagta
     2941 caacggtcag tgcagcaagg gtgaccagta ctgtcggttt atgaatatga ttacgaaaca
     3001 taccccgcaa agttggcaat tattaattca tcaggcaata gcaatcatgg aagatatgaa
     3061 aaaaagggtg tccaaatacg gaaatcagga ataaatgaac agtaatcccg aaagagaaag
     3121 ggtgcccttt tgagacaccc ttttcaaagc aggcagtcgg cagtacaagt tggcagtcag
     3181 tcagatggtt accgggacgg ctgaattcag gagtgactgg ctgatttcat ccggtgttgc
     3241 ttcatgatcc ggaaggttgc cttcgaagta cttttcatag gcagtgatgt caaaatggcc
     3301 gtggccgctg aggttgaaga gaattgtcct ggaaactcct tccaggtcag ctctccttgc
     3361 ctcatcaagc gctccggcga tggcatagga tgattcggga gcggggagga ttccttccgc
     3421 acgggcaaac aagatggcag cctcgaaaca ctcgcgctga aacttggcct tcgcctcaac
     3481 gaatccgtcc ttccgcagct ggcttaccag cactccggca ccatgatagc gcagtccgcc
     3541 ggcatgaatc ttgtcaggaa tgaagttgtg gccgagggtg tacatgggca gcagcggtgt
     3601 catacccgac tggtcgccga agtcataggt gaatttgcct ttggtcagct tggggcatga
     3661 tgccggttcc accgccacca gcctcgtctt tttaccgttg acaaagtttt ctcttaggaa
     3721 tggaaaggca atccctgaaa aattcgatcc ccctccgaag cagccgataa ccacatcggg
     3781 atagtctcct gccttctcaa gctgtatcat cgcctcctgg ccaatgattg tctggtgaag
     3841 gatgacatgg ttaagcacgc tgcccagtga atacttcgtg taggggtcct gtgccgctat
     3901 ctccatggct tcggagattg ctatgcccag actgcccgga gaatcgggat ctttctcaag
     3961 taccttcctg ccagcatcag tcatggtgct gggcgagggt accaccctgg cgttgtaggt
     4021 gttcatcatc agtttgcgtc ccggtttctg gttatagctg accctgacca tgaagacaag
     4081 cagttcgagc ccgaagtggt tgcaggcaaa gctcatggcg ctgccccact ggcctgcccc
     4141 ggtctcggtg gtgatgcgtc ggacaccttc ctgcttgttg tagtaagcct gaggtatggc
     4201 agtgttggcc ttgtgcgagc ctgaatagtt gccgccctcg tacttgtaat aaatcctgct
     4261 ctttgtcccc agggccttct caaggcttcg ggcccggaac agtggcgacg ggcggtatgt
     4321 gtagtagaga tcgactacct catcagggat ggcaatatat ctctcggtcg atacttcctg
     4381 ctttatcagt tccatcggga atataaccga caggtcttca ggacctaccg gctttcccgt
     4441 tgccgggttc agcgggggaa ggggcttgtt gggcatgtca gccatgatat tataccattg
     4501 gcggggcatt tctttctcag tcagcagaat ctttgtaatc ttttccattt taagtgttgt
     4561 ttatgagggt taaaaaaaca agaggcacgc tgtgatcagc gtgcctcttt gtataaatat
     4621 cctgtacggg ttatataacg gcgtgattca cagcttcatc ctatgagctg caccagcgcc
     4681 agttgtttaa cctcatcgta ttcatcaatg caaacataat aattattttc agtagcaacc
     4741 tattcagaag agatttttta aagcataacc cccaaaaaac tttcaaccct gttaatgtcg
     4801 tgagagcagc acttcctcta tttcagagag attgacctgt ttagccctga gcagaatgag
     4861 caggtgatag agaaggtccg ccgcctcgtt tttaaagagg ccgatattgt cacccatagc
     4921 ttcaataacc agttcaaccg cttcctcgcc cactttctgg gcaatcttac cggttccctc
     4981 attgaagagc ctgctggtat atgacccgct gctgtcctct tcaatcctgc tgttgatgat
     5041 atcttcaagc cggtagataa atccttttgc cgattgttcc ccgaagcatg accatgtgcc
     5101 ggtgtggcat acgggaccgg ccggttcggc ctttatcagg attgtatcac tgtcgcagtc
     5161 agtttcaatt gtcaccaccc tcaggtaatt tcccgaggtc tcacccttgg tccagagcga
     5221 ttgccggctg cggctgtaga aggtcacaag cccctcatct tccgttttgc ggtacgcttc
     5281 ctcattcatg taacccgcca tcagcacctg cagggtggtg ttgtcctgta tcacgacggg
     5341 caccagcctg tcacccttat tgaagtccat gttcagcatc ttatgtcaat gttattatgt
     5401 aaaagatatt ttttcagttc aggaatcttt attatcccgt aatgaaatag tgtggcagcc
     5461 agtgctgccg tgcagccagt ctctgcgaac acctccctga agtgttcagg tgagcctgcg
     5521 cctcctgatg cgatcaccgg gatattcacg cagttgcata ctgccgacgt tatgtcaagt
     5581 gagaatccct cgccggatcc gtcgcggttg acggaggtga ggaggatctc gccggcgccg
     5641 agccgttccg actcacgggc ccaggtgatg gccggaagtg ttgtgacgcg cttgcctccg
     5701 tgggtgcaga ccacccatgt gccatcaaca agtttagcgt caattgccac caccacgcac
     5761 tggctgccgt actcatctgc cagttcggca atgaggggcg ggttaagtat cgcggcagta
     5821 ttgacggaga ccttatcagc ccccgatctg atcagcatcg agacatcctc ccgggtggtg
     5881 atgccacccc ccaccgtgaa ggggatgttt attgcctctg ctatcttttt taccagaggg
     5941 gctagggtct tccgccgttc atcagttgcc gatatatcaa ggaaaaccag ctcgtcagcc
     6001 gactgttcag cataggcacg tgccagctcc acagggtcac ctgcatcacg gaggttgacg
     6061 aaactgatcc cttttactgt gcgtccgtcc ttaatgtcaa ggcagggtat gattctcttt
     6121 ttcagcatat ctcagcaatt tcgttgtatg ttatccttcc ctcgagcagg gccttccccg
     6181 ttacggcccc ttcacacccg gcatcacgca gctctctgag atcactgaca gcagtaattc
     6241 cgccgctggc aatcacagcc agtccttcca gcgaggtcag ttccctgtat atctccgcgg
     6301 agaccccggt tagcattccg tcttttgaga tatcggtgca gagtgcatag cgaatccctg
     6361 ccagcctgta ttccctgagg aaatcggcaa tgtctctccc ggatgtctcc tgccagcctc
     6421 ctgtagctac ctttctttcc ctgaaatccg ctcccaggat gattttttca ggcccgtact
     6481 gaccgagcca ttgcctgagc aggtcaggac ggtggaccgc aatgctgcca atggttacct
     6541 gtgcagcgcc gctgctgaag gccaggagga tatcctcctc tgttcgtatg ccgccgctga
     6601 agtcgatttc cagtccggtg gcagaagcta ttccttcaag taccctttgg tttgttattc
     6661 ttccggtacg ggccccgtca aggtcaacca ggtgaaggta cctgatgccg ttatcttcca
     6721 tctcccttgc aaggtcaacg ggatcagcac ggtaaagtgt ctttctgccg aagtcgccct
     6781 gggtcagcct gacgcattga ccgtcaatga tgtctattgc cgcgatgatt ctcatagctc
     6841 aaggaagttt ataagcagcc ggtctcctgc ctctgctgat ttttcagggt gaaactgcac
     6901 cgcatggaag ttatcgcgct gcattgccgc cgaaaagggg aggatgtacc tgcaaacagc
     6961 ttttgtgtca ctgttcagct cggcatagta gctgtgcaca taatagaggt catcgtccgg
     7021 ctcaaggcct ttcagcagcg gggaggagga gaggctctca aagttgttcc atccaatatg
     7081 cggcaccctt tcctccggag gaaatctcct gacaacggcg tcaaagatgc ccagacattc
     7141 agtattattc tcctctgagc gggagcacat cagctgcagg cccaggcaaa ttcccagcac
     7201 cggctgtttc aggggcagga tcagcgaatc gaggtccctt tccctgaggt ggctcattgc
     7261 cgaacgtgct tcacccacac cgggaattat caccctgtca gacttcagca gtatttcagg
     7321 atcggatgta accacagaat catgccccag cctccgcagg gccacctgta cagacattgt
     7381 attgccggaa ttgtacttaa gtattgctat cataatgttc ccttggttga tggcaggttt
     7441 ccgctgcctg tttttaccac agccattccc agtgccctgg cgaatgcctt gaagattgcc
     7501 tctgccttat gatggtcatt atcacctgtg gctctgacat gaatattgca ttttgcgttg
     7561 tcactcagtg acctgaagaa gtgtcggaac atatccgttg agagggtgcc tgcacggtct
     7621 gaccggaatg aggcgttcca ttcaagccag ggccttcccc ccaggtcaat cagtacttct
     7681 gcagcagagt catccattgg cagtgcaaag ccgtaccgcg ccattccttt ccttttcccg
     7741 agtgcccggt caagagcctg tccgagtacg atggctgtat cctcaaccag gtggtgctca
     7801 tcaacatgaa ggtcgccgga agccttcagt gtaatgtcaa tgccggcgtg cctggcaacc
     7861 tgctcaagca tatggtcgaa gaagccgata ccggtgctga tagagccttt cccggtaccg
     7921 tcgaggtcaa tctcaatgga aatgccggtc tcatttgttt cccttgaaac tgacgccctt
     7981 cttgggatcc cggtcaggaa gcgtcttatc tcctgccagt cgcgggtgca gagcgaagcc
     8041 tccccgttct gcctgtcact gacataaatg acccggcagc cgaggttttt tgccagttct
     8101 atgtctgtga ggcggtcacc gatcacgaag gaagagccga ggtcgactcc gtctgcaaga
     8161 tacctggtca gcattgccgt acccggttta cgggtgggag ttctgtcaga ctcaaatgac
     8221 cggtcaatga atatctctgc aaatctcacc ccttctccgg caaggatgct gagcatcttg
     8281 ttctgcggcg gaaggaaagc ctcctctgga tatgcggggg taccaagacc atcctggttt
     8341 gtaaccatca ccagttcgaa gccggtctcc cgggagatgg ctgagagagc tcctatcacc
     8401 cccggcatga attccagttt ttccaggctg tcgacctgtt catcttccgg ctccttcagg
     8461 atggtgccat cgcggtctat aaacaatacc tttttcatat acttaaactt tcaagttcgc
     8521 ttatcagttt ttcgttttct tccggcgtgc cggtggtgat ccggatgcat ccggggatct
     8581 cacggttccg gtttctgact atgattttcc tctcagcaag ggtattgtat atatagtctg
     8641 catcctttac ccttacaagc aggaagtttg aatcagaagg ccagaccttc tccactacgg
     8701 gaagcgcagt tagtcgtgag gcaagttttt cacgctctgt gattatcgac tgtacacgcc
     8761 gggccacagc atcggcctcg ttcagcctca ggagcgcggc ctcctggttg atggtgctga
     8821 tgttataggg tggcttcatg gcaaagaaat acctgagcag gtcagcatcg gcaaatgcta
     8881 ctcccactct tgccgctgcc agtcccatcg ccttgctgaa tgtctgcatc actatcagat
     8941 tgtcaaattc gccaatgcgc tctgcgaagg atggcttccc actgaagtcg gcataggctt
     9001 catcaataac cactatcccg ttaagttccc tgatgatggt ctcaactttt tcaggattca
     9061 tgcagtttcc ggtgggattg ttgggtgagc agataaagac aagtttaagg gtatcttccc
     9121 tcagcaatgg ttttgcggcc tggaggtcga tattgaatgt gtcatcgagg ggaatcctga
     9181 caattccgat gtcatttacc gaggcggcca cgccgtacat gccgtatgtg ggggtgaaga
     9241 tcattgccct gtcctttccg gggttgcaga agatacggaa gcagaggtct atgatctcgt
     9301 cactgccatt tccgaggaat atattttcag caggaattcc ctttttccgg ctgatggttt
     9361 cgcgcagttc acgctggcgc ggatcggggt atctgttgag cggcccatag gggctctcgt
     9421 ttgcgtcaag gaagatcccc tcgctaccct catactcatc ccttgcgcat aaataggggg
     9481 taagcttaag aacgtttggc cttacaagtt tatctgttcg tttcatcatc aaggtcttat
     9541 atcaatactt cactgctgcc gggtgggttt ctgctgtctg atttttgact tcaatgcttt
     9601 tgcgggtaat tcaaccattt cagtcttatt ttcacttttt ctgcgtgttt ttatcaaggt
     9661 aatcaagcct gaccgaaact gctgccgcat gtgcaccgag cctctctgcc tctgccatca
     9721 gtaccgtgtc aggcccgatg ttccggatcc cctctgctgt cacttcctgg aagaagatcc
     9781 tcttcatgaa actgccgatg gtaaccccgc tgaagctcct ggcaaatcct cccgtcggga
     9841 gggtatgatt cggtcccgtc atgtagtcgc cggggctctc gctgctgtag ttgcccagga
     9901 acaccgagcc tgcgctggtc acccgttctg atgtccggat cgggtcaggg gtggcaatta
     9961 tcaggtgttc aggtgcgtaa aggttggctg cctccaggca tttttcctct gattcaagga
    10021 gtaccaggga gctgtttccg agcgacctgg cagcaatgtc tttccgcggc aggagtgcaa
    10081 gctggcgttc tgtctccttc tccacgaggg ttatcatctc gcggctgtaa gtgagcatta
    10141 tcacctggct gtcggtgccg tgttcagcct gggagaggag gtctgccgca ataaaggcgg
    10201 gattggcgtt gctgcctgca atgaccatca gttcgctcgg cccggctggc atgtcgatgg
    10261 cgctgccctc gagctgcaca atctccttgg cgcgggtgac aaaccggtta ccgggcccga
    10321 agatcttgta aacagccggg attgattccg taccataagc catggcagct attgcctggg
    10381 caccgccggc acggaagacg gctttcactc cggcaacctt tgccgcatac agtatcagag
    10441 gattgatttt accgtccctt ccgggtggcg tgcacatgac tatctccccg caccctgcca
    10501 gccgggccgg gataccaagc atcagtacag tggagaacag gggagcggtt cctcccggga
    10561 tatatagtcc tactttttct atgggtaagt cgcgatacca gcaacggaca ccgggagcag
    10621 tctcgatgat ctccggggcg gtgggggtga cctccggagt ggaaccagtg acctccggag
    10681 tggtccgctt agcctccggg gcggtccgtg taatcctggc agttccggca cctgcaaggt
    10741 ggaatttctc aatatttgag gcagctgaca ggatcgcctt tttcagggca gggctgacct
    10801 cacattctgc ctgctcaatc tcgtcagctt caaccctcag ggaggctgcc gcaaagccgt
    10861 caagtttttc cgagtacttc ctgagcgcag catctccacc attcataact gctgagagta
    10921 tctccctgac cttcatgtca atctccctgt taccatccga gggtcgtgcc agggctgccg
    10981 ccagctcagc ggcaccgggg tatagcaata ttctcatcac acaatcattt tttcgatggg
    11041 tattacaagt atcccttcag ctccggcaga tctcaggcgg tctatccttt cccagaactc
    11101 atcctccctg acaacggagt ggaggctgca ccatcccttc tctgccaggg gcagtattgt
    11161 ggggcttttc atcccgggaa gaattctgca tatctccggt actgctgctt ccggggcatt
    11221 gaggagtatg tatttgtttt cactggcgtt tttcacagcc ctgatgcgga agagcagctt
    11281 ttcaagtatc tccctcttct ggccgctgat gttccggttg gcaatgataa ctgcctggct
    11341 cttcatgact gtctccacct ccttcaggcc gtttgtcagc agcgtgcttc ccgaactgac
    11401 gatgtcacat ataccgtcag ccagcccgat gccgggagct atctccacac tgccgctgag
    11461 ttcctctatc tcagctctga tgttccgtga tctgaaatat ccggttagca gtgacggata
    11521 gcttgttgcc acccttttgt tcatgaaata gcccggaccg gtgtaattat cctctttagg
    11581 tattgcaagg gagagtcggc atccgccgaa gccaagctgt tcaaggatat ccacctccct
    11641 ctgcttctcg aggactatat tctccccaac gatgcctatg tcagccacct gctgttcaac
    11701 gtactgaggg atatcgtcat cgcggaggag gagtatctct gccgggaagt taccagcaac
    11761 cactttaagg gttcctgcgc cgttgctgat ccttatcccg cattcgtcaa agagtttccg
    11821 ggagctctca ctcagtctcc cgcttttctg gattgctatc ttcagtatgc tcattccgca
    11881 ttttgtctta tgcctgagca aaccacggag caacaaaaaa cccgcctgat ctgctcagac
    11941 gggtttatga tattcagttt tcaccacaca ccattttcat ctcgcctgag agcaagtatg
    12001 atgatgatga tggtgatgta tcctgtttgt gttcattgtc aattgtccgg tgcaaatata
    12061 cttatttatt gaatctcctg ccggaaactg aaaaaaatca acaggaatca gtccggcgcc
    12121 ttcggattcc ctttattctt cgtccgggat cttaacgtga aagcgcctta acctgttcac
    12181 agatcctctg ccccatcgct gtggttgtaa caggattatc atgactcaga tccggggtta
    12241 ccaccccttc ccttatcgtc tcctccaccg ccctggtgat cagtcctgct tccttcacaa
    12301 ggccgaagct aagttcgagc atcattgcag cggatagtat cgttcccacg gggttggctg
    12361 tattttttcc tgccgcctgc ggaaacgagc catggattgg ctcatagaga gaggcagaca
    12421 gccctattga tgcagacggc agcattccga gcgatccggt gatcacactc gcctcatcag
    12481 agagaatgtc tccgaacatg ttctcggtaa ctatgacatc aaatgctgac gggttcctga
    12541 ttatctgcat tgcagcattg tcaacgaaca tgaattccat ttcaatctca gggtattcag
    12601 caacaatctc ctttgctgtt tcgcgccaga gacgagatgt agcaagcaca ttagccttgt
    12661 caaccacagt cactttcttc cttcgttgga cggcatacct gcaacccagg tgcaccacac
    12721 gctccacctc atacctgctg taggtgcagg tgtcaaatgc ggtcagtccg tcttcagaac
    12781 ggccccgtgg tttaccgaag taaatgccgc cagtcagttc acgtatcatc acaatatcgg
    12841 tccctgccac tatctcctcc ttcagcggtg aaatgcctgc catcccgggt atactcttca
    12901 cgggccttac attggcatag agtcccagct cactcctcat cctcagcaga ccctgctccg
    12961 gccttacctt tgcagaaggg tcattatcat atctggggtg gccgatggcg ccgaagagga
    13021 tagcatctga cctgcggcac aggtgaaggg tctcatcagg gaagggattt ccggtatggt
    13081 cgatggccac agcccccacc atcccttcag tgaagctgaa ggtgtggccg gtaacctcac
    13141 cggtgacatt caacaccctg actgcctgct caatcacttc cggacctatg ccatctcccg
    13201 gaagaagcgc tatcttataa ttcattcact atttcaatta atttgttgta ttctttattt
    13261 gtctcattgt tgcagatgat gttgagcatt ttttccgttg ccttgatggc tgattcggtc
    13321 tgatcggcat caagccccct cgttctgaac tccctgccat tatggttcca tctgataacg
    13381 gtttcgacaa gggcgtcggt ttttcctccc gggggaattg acacctcgta atctgtgagc
    13441 aggggcaatc cggtcccaag cgactcatag atctttctca gggccttcat gaatgcatca
    13501 tactgtccgt caccggcggc ggtctcctgg tgaaggattc cgtcaatttc cacctgcacg
    13561 cttgccgagg gcttaaggcc gcaggtgaga ttaagcgagt agttccggat gaaaaccctg
    13621 tagccggaac gatcatcgcc aagaacatcc gcaatgatga aagggaggtc atcgcgggtg
    13681 acactttcct tcttgtcacc cagttcaatc accctctccg taaccctggc aatcagatcc
    13741 gggctcagtt ccagcccgaa ctcctccagg ttcttgcgga ttgtagcttt gcccgactgc
    13801 ttcccgagtg catattccct ctttctccca aatctctccg ggccaagggt actacagtaa
    13861 agattgtcct tccggtcacc gtcagcatgt acccctgatg tctgggtaaa gacatttgcc
    13921 ccgatgaccg gcttgtttac aggtatccgt attccgctga atttttccac catccggctg
    13981 atacgtgtaa tctcatactc gtttacatcc gtttcggcat cgagatggtc acgcagcaga
    14041 gcgataacgc ttgagagcgg tgcgttgccg gccctctcgc cgaggccgtt cacagtggta
    14101 tgcacccctc ttactccggc cttaaccgcc gaaaaaatgt ttgctaccga gaggtcatag
    14161 tcgttatgag gatgaaaatc aaaatggact tccggctgcc tggcagtcat ctgcgaacag
    14221 aagttaaatg tctggtcagg attcagtatc ccgagggtgt caggcagcat gatccttttt
    14281 atgccgcttc cggccagggc gtcaaccatc cggattacgt aacctggcga tgagagcatg
    14341 ccgtttgacc agtcttcaag gtagatattg acaatcatgt ccctggtctc tgccatcgac
    14401 acgatatcaa ggatgtcaca taaatgctca tcaggggttt ttctcagctg tttttccagg
    14461 tggcgcgatg agcccttgca gaggaggttg atcacccttc ctcccgcctc cctgatccag
    14521 tcaagtgatc tggcaccgtc aacgaatccc agcacttcaa tcctgtcagc catgccgttc
    14581 tgttccgccc acgaggttat aagccgcact gcctcctgtt ccccgggcga gactcttgcc
    14641 gaggcaacct caatacggtc ggtcttcaga tcacgaagaa gcattgtggc tatgtgcagc
    14701 ttctcatgag gcgaatagga aacgcccttt gtctgctcgc cgtccctgag ggtggtatca
    14761 agtatttcca ctttcacgcc tgcctgtttt tctcaaacgc cattatatcc tctttcatcg
    14821 aaaggagata atcaatatta tcaagcccct ttatgaggca ttcctttctg aaaggattta
    14881 ccgggaaacc ggcctgtaat ccggatccgg gaacagtgat aacctgtccc ggaaggtcta
    14941 cccgcactat ggtttcaggc ttggaggtga tcagtcctgt cagctgcgcc aggaactccg
    15001 gtgtgaccgt gactgtcagc aacccgttat tgagcgcgtt gttcatgaaa atatcggcaa
    15061 aggagtcaga caccaccgcc ctgaaatcat ggccggtgat ggcccagacg gcatgttccc
    15121 tgcttgagcc gcagccgaaa ttgcttcctg cgatgagtat ttcaccccgg aattcaggcc
    15181 tgttgagcac gaatccggga acggggttgc catcagcgtc atatctccag tcgcggaaca
    15241 ggttgttacc gaatccctcc cttcctgttg cggagaggaa gcgggcaggt attatctggt
    15301 cagtgtcaat attggccagc ggcaggggaa cggctgttga ttcgagtatt gtgaattctg
    15361 ctcttttcat gtgtttatca gcatcttatt ttttttggcc gaatggaact cgaatgaaat
    15421 atgaccctca attctttttg taagttcctc atggtgattt cctgtgtctg tttttcttca
    15481 tttactgatt ccacacaagt tccctcgggt ctgtaatttt tccggtcacc gctgctgccg
    15541 ctgccaccag cggcccggcc agaagtgtcc ttgctcccgt tccctgccgg ccctcgaagt
    15601 ttcggtttga ggtggagacc gcatattttc ctgaagggat cttatcctca ttcatagcca
    15661 ggcacgagga acagccaggt tgtctcatct caaaaccggc aagcctgagt gtctccgtga
    15721 gtccttcgcg ttctgcctgc cttgccacct gctgtgatcc cggcactatg agggcagtga
    15781 caccggcagc tttctttttg cctgcaacag ctgcggtaaa gagcctgaga tcctctatcc
    15841 gtccgttggt acaacttcca agaaagacgt agtcgattgg gtggccaagc agcttcatcc
    15901 cgggtttgaa tcccatatat tcaagcgatc tgagatacga gccgtttgca gaggagcctg
    15961 cataatcagg tgatggaatt accccgttga cggccattcc catgccggga ctggttccgt
    16021 aggttatcat cggggttact ttggaggcgt caaaggtgac ttccctttca aagccggctc
    16081 cgcggtcact cctgagagag tgccagtgat gaatcaggcg ggcaaattcc ctgttatcag
    16141 atacggaagg tatctgagcc agccagttga aggtcacctc atcgggagcg ataagcccgc
    16201 cgcgggctcc catttcgatg ctcatattgc atacagtcat gcgtgactcc atgctcagct
    16261 tcctgacagc ctcgccggca tattccacga agtagcctgt ggcgcctccg gcagtcagtg
    16321 aagctatgat atggaggatc aggtccttgg aggtgactcc ctcatgcagt tcccctttaa
    16381 cattgatcct catctgcttc ggtctggtct ggagaaggca ttgcgttgca agcaccatct
    16441 ccacttcgct ggtgcctatt ccaaaggcaa ttgttccaaa agctccgtgg gtggaggtgt
    16501 ggctgtcgcc acagacaatg gtcattccag gaagggtgaa tccctgctct ggtcctgtga
    16561 cgtgcacgat gccgtttcgc ggatcaccca tcccgtaata cctgatacct gcagcacggc
    16621 agttctcctc cagtgtctca acctgcaacc gggacacagg atctgctatg gggaggtgct
    16681 ggttgaccgt gggtatattg tggtcagaag tggcaatgat cctctccggc cggaaaatat
    16741 taatacctct ctgccgcaga ccgttgaaag cctgcgggct ggtgacctcg tggatatagt
    16801 ggcggtctat gtacagcaca tccggcccgc cgcttattga cctgacaacg tgggcatccc
    16861 atatcttgtc aaagagtgtc tttccgctca ttatcaatct ttgaaattgc atcgatgaat
    16921 gcctcaactg atgctgtgac tatatcagtg tttgcggaga aaccgtaaaa aatgtttccc
    16981 tcatgctcaa cctggatgtg gaccttgccc atgtcatcac ttccccgggt aatggcctgg
    17041 atgaggaatt cctcaagatg cacattctcc tgtatcaggt gtttcacagc ggtgaatgca
    17101 gcgtcaattg gcccgttgcc gggagccgtt gaggtgtgca cctcaccatt gaccctgata
    17161 cccacggtgg ccacaggcat tacggagttt ccgcagagga cctgcagcga ctccagcttg
    17221 agccgttttt ctccccggaa gacttccccc gacaatatcc tgatgtcttc atcattgatc
    17281 tcctttttct tgtcggccag catcaggaag cgatggtaga tctcatcgag ttcctccttg
    17341 cccagctcaa tacccagtgc ttcgagccgg tgattcagtg cagccctgcc gcttcgggcg
    17401 gtaaggatga tcgatgactc cctgactccc acctcgttcg gatcgattat ctcatagttc
    17461 tcgcgttttt tcagcacgcc gtcctggtgt ataccggagg agtgtgcaaa agcattccgt
    17521 cctacgatgg ccttgttggg ctggacaggc atgctcatca gtgtggagac cagcctgctg
    17581 gtgctgaaga taagtttcga cctgatggcg gtggtgaggt ttaggtcatg gtggctttta
    17641 atgatcattg ccacctcctc gagtgaggtg ttgccagccc tctcgcctat gccgttgata
    17701 gtcacctctg cctgccgtgc accgttgatg aggcccatca tggtgttggc ggtggccatg
    17761 cccaggtcgt tgtgacagtg ggtggagagg atggccctgt caatgttcgg cacattctcc
    17821 atgagatacc tgatcttgct gccatactct tcaggcaggc agtaacccgt agtatcaggt
    17881 atgttgacaa ctgtggcgcc ggcgttgatc acggcctcag tcaccctggc gagatactca
    17941 ttgtcgctcc ggcctgcgtc ttctgcatag aattccacgt catcggtgaa tttcctggcg
    18001 tacctgactg catctgcagc tcttttgatt atctcttccg gggtggtcct gatcttgtac
    18061 tgaatatgcc acggcgaggt gcctatgccg gtgtggatcc gctttctttc agcatactgc
    18121 agtgcttcgg cagccacttc tatgtctttt ttcaccgccc gcgtaagtgc gcatataacc
    18181 ggctccctga cagctttcga tatctcaaca acagaacgga agtcgcccgg actggaaacc
    18241 gggaatcctg cctcgattac atcaacaccc agtgcttcga gcgactttgc aacttcgatc
    18301 ttttcaacgg tgttgagctg gcatcccgga acctgttctc cgtcacggag ggtagtgtca
    18361 aagaaaatca ccctgttctt gtccatgatt tccgttttga ggttatttgc cgtcaggcct
    18421 cagtttcctt actgcagcgc ctgctttcca gagctctgat gatcccatct cagccagttc
    18481 ggcattaagc tgttccctgt atccgggtgc gccggtagag gtcagcaccc tgacgcactc
    18541 ctccccggag cttacttttt catacagttc cctgaagacc gggagggtag cttccctgaa
    18601 ccttggtttc cagtcgagtg ccccgcgctg tgccgtggcg ctgcaattgg catacatcca
    18661 gtccatccct ttctcgtcga caagcctgat caggctctgt gtcagctcct ccaccgtctc
    18721 gttgaatgct tccgatggtg agtgaccgtt ttcgcgaagc acctggtact gtgcctccat
    18781 gattccctcg agggctccca tgagtacgcc gcgttcgccg gtcaggtcgc tgaagacctc
    18841 cttctcaaag gtggtgggga agaggtaccc ggagccaatg gcaataccca gggcaatgac
    18901 ccgttctccg gccctgccag tatggtcctg gaagaccgag aagctggagt tgatgccggc
    18961 tccggcaagg aagttgcgcc tgacgctggt gcccgagcct ttaggggcac agagtatcac
    19021 gtcaatgtca ggcggtggta tcacccccgt atgctgcttg tatgtcactg agaagccatg
    19081 ggagaagtaa agggcctttc cggcggtgag atgtggactg atcaccggcc acagtatgcg
    19141 ctgggcggca tccgatacca ggtactggat gatggtaccg cgggaggcag cttcctcaac
    19201 ggagaacagt gtccggcccg gaataaatcc gtcagcaacg gccctgtccc agtctgccct
    19261 gaactccggg gcctggccga caatcacatt aatgccgttg tcacgcatgt tcatggcctg
    19321 tgccggtccc tgaaccccgt aaccgatgac ggcaacaacc tcatccttaa gtgcctcgtg
    19381 tgcttttgac agtggaaatt cctgccttgt taccacattt tcaattactc ctccaaaatc
    19441 aatctgtgcc ataatgaata tctgttaggt tagtgtctgt ttcagttgta gaattcaagt
    19501 gcctcctgcc gggttcccct ggcttcttcc atctcacgga ggtatgcgtt aagctcttta
    19561 acctggcgtg tgacggccac ccgacccgag ctgacgaact gcagtacacc gcaaggttca
    19621 agaagcgcaa gaagttcctt tatctccgcc ctggtgccgc tcttttccac caccatgtat
    19681 tcggacttta cctccagtat ccttgcgtta tgcttcctga caatgtcact gatcatgctg
    19741 cctgacatga gtcccctggt ggtgaccttg tagagagcca tctcctggca gattatctcg
    19801 gatgatgtgt gtacgaatac cttgagcacg tcaaccacct tttctacctg gcgggcgact
    19861 ttctgcacca tctcaggcgt ggtgcgcaca accatagtca gcatctgcac tcccggtacc
    19921 gctgattccg aggcggttat actttcaatg tttatctgcc ggcgtgacag tatgagggta
    19981 atcctgctga ggattccgat atggctttcg ctgaatagcg atattgtgaa ttcctgtttc
    20041 atgacttgct gttgtatgta agtgttactt cagatactga tgctcccggc tcgaccatcg
    20101 ggaggatgtt tgccgtgcgg ctcacctccg ccaccaggat gaaggggccg tctgagtcaa
    20161 gcatctctgc ggcagatgcc ttcagttctt cgcggttttt tatctttcgc ctggggatcc
    20221 cgtaggccga agcaagtgcc acaaaatcgg ggttgaccat ctcagttgag gcgtaacggc
    20281 tgttaaagaa catctcctgc cactggcgca ccattcccag gaagctgttg ttcagaagaa
    20341 ctattttcac aggaatgtga tactgcatta ttgttcccag ttcctggagg gtcatctgga
    20401 tgccgccgtc accgataaat gctatcacct gccggtcaga tgtgccggtg gcggcgccca
    20461 cggcagcggg cagcccgaag cccatcgttc ccatcccacc cgaggtaacg agggtgttgc
    20521 tgtcagtgaa gtggtaatag cgtgccacag ccatctggtg ctggccaaca tcagtcacca
    20581 ccaccgcgtc tctgccggca tgctccgaaa ttaccttcac cacttcaccc atcttcagtt
    20641 cgccattttc cggttccgtc tccggcctga tgactttttc gtgttccgta ctgtcaagaa
    20701 tccggaactc gcgtatccat tcctccctct ttctttccct gatatgggga aaaattgctg
    20761 tgagaacttc tttcacatca ccattgattc caagatctgc cctgacattc ttgtctatct
    20821 cagagctgtc tatctcgatg tgtatcaccc tggcctgagg tgcatatttc ttcaggtttc
    20881 ctgtcacacg gtcatcaaac cgcattccgg cagcaatgat caggtcagcc ctgtttgtca
    20941 gcacattggg tgcatagttg ccgtgcatgc ccagcatgcc ggcgaaaagc ctgtgatcat
    21001 ggctgaatgc cggtatcccg agcagggtga cagccacggg tatgcctccc ttttcggcaa
    21061 gggatttcag ctccgccact gctccggaga tgattacccc gtggccggcc agaatcaggg
    21121 gcctctctga atggttgatc atcacagcgg ctgattcgat atccctgttg acaatcggat
    21181 tcaccggatt gtagctgcgt atgtaggtgc atctgcggta acggaagttc atggtctcaa
    21241 tctgggcatc ctttgaaatg tcaaccagaa cggggccggg ccgccctgtg cgggcgatgt
    21301 aaaatgcacg ggcaatggtg tcaggtattt ccgcggcttt cttcacctgc gcattccatt
    21361 tggtaaccgg cattgagatg cccaggatat ctgtctcctg gaaggcgtca gtgccaatga
    21421 gaggagatgc aacctgtgcc gtaatgaaca ctacgggtac cgaatcaagg aaagcgtttg
    21481 caatgcctgt caccaggttg gtggctcccg ggccggatgt ggcgaagcag acacccggtt
    21541 tgcccgctac catggcatag gcctgggctg catgtgctgc accctgctca tgcctcgtga
    21601 gtatatgcct gagacttccc gtgtagtcta gcagcctgtc atatacaggc ataatggcgc
    21661 ctccggggta accgtagatg gtatcaaccc cctcctcgac aagtgatttc aggagcgcct
    21721 cggcaccggt aattgcttta tgattattgt cttctgtcat tttccgcatt ttttatatct
    21781 cccgtattgc accccggtct gccgagctga cgtgagccgc ataaaccttc agtgcgcctg
    21841 aaaccgtgcg gaccctgttc tccggtgtga atgccgcggc tcccctggct ctctctgccc
    21901 tgcgtcttgt ctcaaggacc gtatcatcca cagccagcct gatggttctg gcaggtatgt
    21961 caatcattat cctgtcgcca ctgcacacca gggctattgt tcctccggcg gcggcctcgg
    22021 gggagatgtg accggccgaa agccctgctg acccgcctga gaagcggccg tcagtgatga
    22081 gggcacaccg gcggtcaaga cctttagacc tgagatatga tgtggggtac agcatctcct
    22141 gcatccccgg gccgccgcgg ggcccctcgt aacggatcac cacgacatca ccctccctta
    22201 ccctgcctcc gagaatgccg gccacagcat cctcctggga gtcaaaaacc actgccggtc
    22261 cgctgaatcg catcattgag ctgtcaatgg ctgccgtctt caccatgcat ccgtccggag
    22321 caaggtttcc gtaaagaacc gccagacctc cgtcattgct gtatgcatga cctgcatccc
    22381 ttatcgctcc ggtgaccctg tcagtgtcat gagagccgta cctggcttca gaggcgaatg
    22441 gccggggacc ggctgacctg tcgggagagg agagaaagag atcgtgtgct tccggcaaaa
    22501 cgaccgggct caaaatatcc cagccggcca gcgccgaatc cagtgacggg caatctatcc
    22561 tgcgcacgga agcatcgagc aatcccgccc tgttaagttc tcccattatt gcaaataccc
    22621 ctccggcacg gttgacgtct tcaacgtgat accccgaggc aggggagacc ctgcaaatgt
    22681 tcggaacacg gcgtgagagg gcatcaatct ccttcattgt gaagtcaacc ccggcctcac
    22741 gtgctatggc caggagatgc aggacggtat ttgtcgatcc tcccatggcg atgtcaacgg
    22801 tcatagcatt catgaatgcc tccctggtgg ctatcgagcg gggtaatact gtttcatctc
    22861 cgtcaatgta gtacctttca gcaagatcag ctatcagctg agctcccctc cggaacatct
    22921 tcatcctgtc aacgtgtgtt gccggcacgg tgccgtttcc cggcaatgcc agccccagcg
    22981 cctctgtcag gcagttcatt gagttggcag tgaacattcc cgagcatgat ccgcaggagg
    23041 ggcatgatga tttctcgagt tcaagaagct tttccccgga aacggtgtca tccgcagcag
    23101 ccaccattgt atcaatcagg tcaacggctc ttccgcccaa aacgcccgcc tccatcggtc
    23161 cgcccgaaac gaacactgcc ggaatattca gtctcatggt ggccatcagc atgcctggag
    23221 tgatcttgtc acagttgctg acgcagatca tggcatcagc acggtgagcg ttgcagacgt
    23281 actccacgct gtcagcaata aggtcgcggg aggggagaga gtagagcatt ccgccgtgcc
    23341 ccatggctat gccgtcatca atggcaatgg tgttaaactc tgctgcaaaa agtccccggc
    23401 gggcaagctc ttccctgatc acgctgccgg cctgctgcag gtgcacatgg cccggcacga
    23461 actgcgagaa tgagttgacg acagctatca ccggcctgcc gaagtgttcc tccttcatgc
    23521 cgtttgcacg ccagaggctc ctggcgccgg ccatcgtcct tccgctcatt gtctgcaagc
    23581 ttctcagttc ttttttcatt acagaagttt tttaaacaaa aaaagctccc cggtttcccg
    23641 ggaagctttc agatattata atcctcacta tatactactc cccggtcatc cgcttatggc
    23701 gatgagaata aacagggcaa taaatagtga tgtgtggtac atagtcttac ttatggttgg
    23761 caaaaataac cattaattta aaaatacaag aggaaaccgg agttatttta attttttaaa
    23821 cccggcctat acctattaat aaatgggaaa ggcaaaaaaa ttaaaattta tttcagaacg
    23881 ccaaggtcct ttccgacttt tatgaatgca tttatggctt tgtcaaggtg ctccgtctcg
    23941 tgggctgccg aaagctgtac ccggatgcgt gcctgatcct tcggaaccac cggatagtag
    24001 aagccgatga catagatgcc ttccttcagg agcctggatg ccatctcctg tgacagtttg
    24061 gcgtcataga gcatcacagc gcagatggct gactgtgtgg gcttgatgtc gaagccggct
    24121 ttcagcatct tctccatgaa atacctggtg ttgtgatgaa gcttgtcctg cagctcgttg
    24181 gtctcggtca tcatttcaat catcttcaga ccggccgcag ccacgagggg agggatggag
    24241 ttggagaaaa gataaggcct ggaacgctgg cgcagcatct cgatgatctc ctttttcccg
    24301 gtggtgaatc ctccgatggc tccaccgaat gcctttccga gcgtaccggt gataatctca
    24361 atctttccgc gaagattgta aagctcggtg acgccgtggc cggtctcgcc aaccacaccg
    24421 gcggagtgtg actcatcgac catcaccatt gcattgtatt tgtttgccag ttcacagatc
    24481 ctgcccagag gtgcaacatt gccgtccatc gagaagacac cgtcagtgac aatcagcctg
    24541 aagcggtgct gctgtgccag ctgaagctgt gcctcaaggt cttccatgtc agcgttctca
    24601 tacctgtatc ggttcgcttt gcagagcctc acaccgtcaa tgatcgaggc atgattgagc
    24661 gagtcgctga tgatggcatc atcactggtg agcagcggtt caaacacgcc gccgttggcg
    24721 tcaaagcagg cagcataaag tatggtgtct tccgtgccga agaattcagc aatctttttc
    24781 tccagctcct tgtgcatgtc gcccgttccg cagatgaaac ggaccgatga catgccgtag
    24841 cc
//
LOCUS       DAOPXU010000034        24595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_2034_length_24595_cov_13.485941, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000034 DAOPXU010000000
VERSION     DAOPXU010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24595
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2034_length_24595_cov_13.485941"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1799
                     /locus_tag="PKK03_05115"
     CDS             93..1799
                     /locus_tag="PKK03_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83810.1"
                     /translation="MKNFTRLFVILLAAVLMSGCSSLDKMKKNANLVKYEITPKVLET
                     HAGIVAAQVKVTYPEKYFDKNTTLKITPVLSYEGGETAFDELLFTQGEKVLANNKSVA
                     WTGGTVNWSGDVNYIPEMKVSEFLLHVDAERKGKTLSFDPIKLADGVIATSTLVEYHA
                     MPISLKDNYQRIVPEQKIADILYSINQSNIRTSELKAADIQALKDYIALVMQNERMEV
                     KGVTNSSYASPDGPLTLNEKLSEARGKAAEKYLQKEYGKIPELAQLFTKQTTAEDWEG
                     FKALVQESSIADKELILRVLSMYQDPAVREKEIKNMSTAYESLAEQILPQLRRSKLIV
                     DVNLIGLSDEEILTAIKGDPSVLSLEQMLYAATLTSDPQEMLKYYQLATEKEPKCFRA
                     WNNVGWAYLQMGNADDAMVALEKAKALKNDDNVKNNLGFAALLKGDVKAASEYFNSMS
                     AATPESKFGLGTIAIHDGKYDQAVNMLGDTPTMNLALAQLLKGDVNKAKTTMDAVKPC
                     KCGAPSYFKAVIAARLDNKDGVINALREAIGYNTKWKNYAMTDLEFAKYFTDDAFIAV
                     TK"
     gene            complement(1867..2397)
                     /locus_tag="PKK03_05120"
     CDS             complement(1867..2397)
                     /locus_tag="PKK03_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNX83811.1"
                     /translation="MRKLTNDELNRKSVTEFRASEKSPFIIVLDNVRSLNNVGSIFRT
                     ADAFLVEAVWLCGITATPPHREIHKTALGATESVEWKYFESSAEAVRVLKERDYAIVS
                     VEQAEGAVSLDRFATEPGKRYALVFGHEIRGVTEEVVNLSDACIEIPQYGTKHSFNVA
                     VSAGIVLWELAGRIKG"
     gene            2499..3689
                     /gene="sucC"
                     /locus_tag="PKK03_05125"
     CDS             2499..3689
                     /gene="sucC"
                     /locus_tag="PKK03_05125"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNX83812.1"
                     /translation="MNIHEYQGKSILQAHGVAVQEGFVAITAGQAVTVAEELRSKFGT
                     EIFVIKAQIHAGGRGKGGGVKIARSPQEAGEVASRMLGMTLVTPQTGPAGKKVHKVLV
                     ARDVYYKGESEIKEFYLSLLLDRRKSAYVVVYSPEGGMDIEEVAARNPEALFTETVDS
                     TCCLHEFQMRTIASNLGLHGKAYREMVTFLKGLWEAFVNSDASLLEINPLLKTSDDRV
                     LAVDCKVTLDDNSLYRHPELAAMRDLNEEDPTEVEASASNLNYVKLDGDVGCMVNGAG
                     LAMATMDLIQLSGGKPANFLDVGGGASPKTVEAGFRIILKDPHVRAILVNIFGGIVRC
                     DRVAAGIIEAYKSIGDIKVPVIVRLQGTNAEEARALIDSSGLKVIAASTFTDAAERVR
                     AALS"
     gene            3777..5789
                     /gene="priA"
                     /locus_tag="PKK03_05130"
     CDS             3777..5789
                     /gene="priA"
                     /locus_tag="PKK03_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005636736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNX83813.1"
                     /translation="MSRQFADIIVPVAVPGSFTYEIPEQLQGTVRRGSLVNVPFGQSR
                     KTAGLVVKVHDIPPAEVSLREVDSLLPGETAVNDRLADFLLWISDYYMAYPGEVMKAA
                     IPSPDDLPGRVMKRRPKEAADLPETEMVMPAELNRVQKEAYDSVKQLFADRETVLLHG
                     VTSSGKTEIYIHLMREQLDQGRQVLYMLPEIALTTQIIERLRKHFGSAIGVFHSRLTP
                     AARRKVWQKVSDGSLRAVLGVRSSLFLPFSHLGLIIVDEEHDSSYKQNDPAPRYHARD
                     AAMMLARIHGGRALLGSATPSLESYHNALSGRYGFVELLTRYGEVMMPEMVIADTRRK
                     GKKKGPSAHFTPRLLEAVEEALKREEQVILFQNRRGFSPFLMCSDCGHVPACTNCSVS
                     YTYHKSINRLVCHYCGKSVRLPSECPDCGSVNISTMGFGTEKIEEEIRLLFPSARVAR
                     MDQDTVRNRGAASDILGSFAAGDTDILIGTQMISKGLDFERLTVVGILDADNMMHFPD
                     FRAHERSFQLMEQVSGRAGRRTRRGKVIIQTADPANMILRQVLKHDYRSMSSAQLGER
                     ALFGYPPFTRIIRIALKHRDLDELTASAARLADALRRHLGNHVLGPEFPMVMQVQKWY
                     IKTVMIKIVSTLSPSKVKELVRRAMDTELKSPRKGLLRIHADVDPQ"
     gene            5845..6216
                     /locus_tag="PKK03_05135"
     CDS             5845..6216
                     /locus_tag="PKK03_05135"
                     /inference="COORDINATES: protein motif:HMM:NF039364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6165 family protein"
                     /protein_id="HNX83814.1"
                     /translation="MKIEVSNGEIADKLTIIELKLERISDPEKLKNLKKEHAILDEAL
                     AGFMKKDDPLYLELYRVNAGLWEIEDRIRDLERKKDFGEEFIETARSVYFSNDLRSEI
                     KRKINQITGSGLVEEKSYEKY"
     gene            6218..7243
                     /locus_tag="PKK03_05140"
     CDS             6218..7243
                     /locus_tag="PKK03_05140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNX83815.1"
                     /translation="MSLLVIRTSSMGDVIMTLPVLRSFAAAYPGKELVFVTKKPFDQF
                     VTGIPGVKVFLSDHHGRHKGFRGLLRVWKDLRSMYHITAVADLHDVIRSKVVRLLFRM
                     GGCHVASIDKERIRRRKLVRGRLTGSLTHTVERYRKVFERAGYPLELVPGPWMFPSAE
                     ERAVAAARTAGMKRPFLGVAPLARHRLKTWPPGKMEAMLRILISRKECTLFLFGSPDE
                     SAAVDEIAARVPGAISMAGKISLEGEIALIADLDLMVAMDSSNMHLASMLGTDTLSIW
                     GATHPRAGFSAWGMPPENQIQIPDTELTCRPCTIYGKGTCRRGDLACLEWLTPEMVLQ
                     KILNKLK"
     gene            7247..9574
                     /locus_tag="PKK03_05145"
     CDS             7247..9574
                     /locus_tag="PKK03_05145"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNX83816.1"
                     /translation="MKFTGTNHLIIALAFITLAGAGCRQTPEGTADRISAEPADLVGT
                     LMGTDSEYLLSHGNTYPAVALPFGMNFWTPQTRENGNGWGYTWDDHKIAGIKQTHQPS
                     PWINDYAAFSLMAVTGGNRLTEQARSSWFSHKAEVAKPWYYSVYLADFDITAEVTPTE
                     RAALFRFTFPGSDSSAVVIDAFAGGSMVRIIPGSNRVVGYCRNNHGGVPENFHNYFVI
                     EFDRPFDFWSAALDGSLRPGVTETEGDRAQAVIGFRTKRGQQVTARVASSFISPEQAI
                     LNLERETGGRGFEAVRDAGRERWNTELGRIKVEGGTDDQQRTFYSTLYRTMLFPRMFY
                     ETDSSGNIVHYSPYNGKVLPGRLFTDNGFWDTFRAAMPLITLMYPEMSSHIMEGLVNT
                     FLESGWLPEWASPGHRNCMIGSNSASLIADAYLKGIRGYDIDTLWKAMVKNTLGHGPV
                     HSVGRYGAEYYNRLGYVPYDAGINENVARTLEYAYADWCLWKLAQELGRPKEEIDLYG
                     ARARNYKNVFDVESRLMRGKNLDGTFQSPFSPYKWGDAFTEGNSWHYTWSVFQDVAGL
                     AELMGGDREMARMLDSVFVVPPLFDDSYYGFPIHEIREMQIMNMGNYAHGNQPVQHMI
                     YLYNYTPEPWKAQMRVREVMDKLYNYTPDGYCGDEDNGQTSAWYVFSALGFYPVTPGT
                     GEYVLGSPLFTRATVTLSDGRKLVISAPENSHDNIFVRDVTFNGKKLNLNFVTHEQLT
                     AGGTLEFDMSSEPDRERGTAPSARPYSMSTHGIQN"
     gene            9593..10504
                     /locus_tag="PKK03_05150"
     CDS             9593..10504
                     /locus_tag="PKK03_05150"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNX83817.1"
                     /translation="MASLNLRGIFPPLPTSFDSDENLLPEKIRYNIERLSQYDLAGFL
                     ILGSNGEQVMLSEPEKIKAIAAAREALPDGRILLAGTGCQSTRETISLTRAAAAEGAH
                     AVLVINPSYYRGNMNHDELVNHYFDVADASPVPVLVYNMPANTGIDMTAGMIISVAEH
                     PNIAGLKESGGNVVKMGEVLGRVKPGFQVLSGGAGFLLPALATGASGGILASANIAPG
                     HCLSIFNAFAAGDMTLARELQHSIIPLNAAVTARWGVPALKAAMDHLGLYGGHVRRPL
                     LPLREEIRKQLLQLLDDRNLNLFGEKK"
     gene            10511..11632
                     /locus_tag="PKK03_05155"
     CDS             10511..11632
                     /locus_tag="PKK03_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNX83818.1"
                     /translation="MKGRKLLMIPGPTEFEPEVMMAVGAPTTSHVAPDFIESFGHALE
                     MMREVWLAPSGQPFIIAGSGTLAMDLAGANLVEPGDRALVISTGYFGDRYAELLKRYG
                     AEVTALKAPAGEVVSDAAVETELKRKRYKLMTFTHVDTSTAVRVDPEPLGLLGEKHGV
                     ITVLDGVCSVAGEVIRQDEWKIDVVFTASQKAVGVPPGLALLVASPRAMEAWKGRKSP
                     VGSYYSDWTHWLPVMKAYEERRPAYFGTPAVNLVSALEVSLRLILKEGMEARFRRHSD
                     RGTAFRKAMRGMGLTMIPASEEVSANTLSAPLYPEGVKGADFMKAIGDSDVIVAGGLL
                     PGIKDTYFRVGHMGAVTNGDILTTVAAIDGALSACGYRK"
     gene            11678..12127
                     /locus_tag="PKK03_05160"
     CDS             11678..12127
                     /locus_tag="PKK03_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNX83819.1"
                     /translation="MSLTEKIAKDLMAAMKAQDKGILEALRAAKTAFILARSERGQDT
                     VLTEDEEVKIIRKLVKQRKESADIYREQKRPDLCEKEENEAAVLERYLPAAMTEQQLE
                     IALREIISRVGAKGPSDMGRVMGAATKELAGKAEGREISAKVKQLLG"
     gene            complement(12142..12744)
                     /locus_tag="PKK03_05165"
     CDS             complement(12142..12744)
                     /locus_tag="PKK03_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83820.1"
                     /translation="MVLQPVTMTALETLKNLVLAPYILKATALISVQRAVGGNQFRHC
                     FSTLGILLDYKFFTDSILLKASLLHDLLEDLPETQVDEIRWIDSDGADVVQLVLEVTK
                     RKDETKEQYLQRLLEYGSRNAMLLKCADRISNLTDLHRDIQSEQKISDYLDQTEKYVI
                     PMARRVNTDMLIELTDLIAKRRRILRMLENDKKADTAPGS"
     gene            12911..14677
                     /locus_tag="PKK03_05170"
     CDS             12911..14677
                     /locus_tag="PKK03_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX83821.1"
                     /translation="MPDKPSRPSGSGLKQMARLYRFLKPYRGRFALGLVFLLLSTAAS
                     LMFPRLLGEMVDQANSGGLTREITRTGLLLLAILVAQSLFAYTRTRLFVVVTEKTLAS
                     IRQHIYNHLIRLPMSFFSSRRVGELNSRISSDISLLQDSLTNTLADLLSQLLVITGGI
                     TLMMISSFRLTIFTLAIVPAISLMAFYSGRAIRRYSKKAQAFVAESNTIVEETLQGIQ
                     NVKAFTNESFESRRYREKTEQVARAGITGGKYQAAISFIVLGFFVSMAAVIWQGATMI
                     AKGQMEAGQLFTFVIYSGFIGGNIAGMAGVYARLQKTIGASENLLQLLDEKTEEIDPS
                     FIPGEAHLLNGEITFEGVGFRYPSRNEVVVLRDVSFEVAPGRQVALVGPSGAGKSTIA
                     SLLLRLYDPTEGRILFDGRDSTGFPLTALRSQMAVVMQDVFLFGGTIRENIAYGKPGA
                     TEDEVIDAAKQANAWDFIRTFPGQLETVVGERGVQLSGGQRQRIAIARAVLKNPRILI
                     LDEATSSLDSESERQVQEALEKLMKGRTSLVIAHRLSTVRNSDHIIVLNDGRIVEQGT
                     HTDLISNGNGLYKTLTELQFSL"
     gene            complement(14761..15417)
                     /gene="pdxH"
                     /locus_tag="PKK03_05175"
     CDS             complement(14761..15417)
                     /gene="pdxH"
                     /locus_tag="PKK03_05175"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HNX83822.1"
                     /translation="MNSIRRMTTLLRNEYHGEGLNLADFSHDPVVQFGRWMEDAISYK
                     VMDPNAVHLATADAEGRPSGRIVLLRDFSPKGFTFFTNYGSRKGDELRNNSHAAMTFF
                     WNELYRQIRIGGRVSRLPSDESDAYFRSRPRESQISAVASQQSRHLESRELLDRTAAE
                     TEEKYRDRPIPRPDDWGGYCLDPDYVEFWQGREHRLHDRLIYTRNSDREGWTIRLLYP
                     "
     gene            15540..>16315
                     /locus_tag="PKK03_05180"
     CDS             15540..>16315
                     /locus_tag="PKK03_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNX83823.1"
                     /translation="MTRILPTFALIITLTVPSVVSAQVPVEVSTEKVVSGGRVYYMHE
                     VQKNQTLYSIAKAYRVSVDALSRENVIPPNGIQTGQVLRVPAPDQQGSAASQPRLPAQ
                     QAAAVTQPRPAAQPSRPALTSGVPGIDISGEKIISGGKTFFMHEVQKGQTLYSIAKAY
                     RVTILDIDRENSIPPGGIQAGQVLKIPASSALMVSEEQTPPSAAAATPDRSTAAAANA
                     SGGNTAAVTNMPRQETPRQETQLPRQETPRQETQLPRQETP"
     assembly_gap    16316..16415
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16416..17278)
                     /locus_tag="PKK03_05185"
     CDS             complement(<16416..17278)
                     /locus_tag="PKK03_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNX83824.1"
                     /translation="MSVLKLLVVDDEPGIRSGTERAMRNFRVSYPFLDEDFTYEIMTA
                     ESGEEALRIIDSTPVDILLLDNKLPGMDGIEVLEYIREKKHDIAVMMITSYASLDLAV
                     KATTIGAHSFMPKPFTPQELRTAVEAITKNLFLRRMTTRMTEEGRQSQFQFLSVLSHE
                     LKSPINAVEGYLNIILEKQVGDSVDDYMVMLERCKERLRGMRALITDLIDLTKIEAGE
                     KNRHITNVDLHQVTKSVIDTIEPLAKQKKVKIHADIPENCFIRADRNEIEIIISNLVS
                     NAVKYNHDEGEV"
     gene            complement(17348..17818)
                     /locus_tag="PKK03_05190"
     CDS             complement(17348..17818)
                     /locus_tag="PKK03_05190"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNX83825.1"
                     /translation="MNEKGNRSSLKENLTKVVAELFSTSGTVRCKTLSVATGVFIGLS
                     PVWGFQTVTAIAASYLFRLNKILTIGSSAISLPPLIPVIIYGSYRLGGLILGSGAIDP
                     DYNSIKDFRDITEGMLQFVTGSLVMALIVSSVAGLTTFLILRRQERKRNRKAGN"
     gene            complement(18231..18671)
                     /locus_tag="PKK03_05195"
     CDS             complement(18231..18671)
                     /locus_tag="PKK03_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83826.1"
                     /translation="METEKRLKMLQLFYAGVLADSVRRYSEAGILADVEAQKEKEQKM
                     AAKGQLAQLRITDPSGIFNVFSEVFGCISWNIEETEEKLTARGHSCLLCAIAKKMNLP
                     KPCNIYCINPVRAQVEGLERGYRLNVRSTLWESDKCEFVVTGNK"
     gene            18748..19515
                     /locus_tag="PKK03_05200"
     CDS             18748..19515
                     /locus_tag="PKK03_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNX83827.1"
                     /translation="MDVQSLQNRMTRLLDSQKPSPEHTDYSILNYHMRLLEQIALMEN
                     SSMTIYDILQKRYVFVRNRFRELIDYDEQAAAEQGYAFFFRLMHPDDISFVLDTSIRA
                     IEHIIGLPPAERKDYKTIFEFRLRNREGKYIRFIQQLVNLELDLQGNMWLILIVMDVN
                     PNQQGSKQMLRSTVNMKTGKVVSFTEEVEERQSQLTKREIEILGLLARGMVSKEIADE
                     LFISVNTVNNHRQRIIEKMDVANTSEALNYATRIGII"
     gene            19530..19718
                     /locus_tag="PKK03_05205"
     CDS             19530..19718
                     /locus_tag="PKK03_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83828.1"
                     /translation="MSDFQMLIDELVKFRDARQWEQFHNTKDLALALSIETAELNELF
                     LWKPVDKSKDTARKYNEL"
     gene            19768..20280
                     /locus_tag="PKK03_05210"
     CDS             19768..20280
                     /locus_tag="PKK03_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HNX83829.1"
                     /translation="MIKLLLGDITRIEVDAIVNAANSSLLGGGGVDGAIHRAGGRAIL
                     DECRRIVERQGGCRTGEAVITTAGKLPARYVIHTVGPVWHGGINNEDQLLTNAYSNSL
                     QLAADNGVKTIAFPNISTGVYGFPKERAAKIAIEAVRKFLKNDKSLREVVFVCFDREN
                     HEIYERLLNA"
     gene            20385..21023
                     /locus_tag="PKK03_05215"
     CDS             20385..21023
                     /locus_tag="PKK03_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83830.1"
                     /translation="MQSAESLRRKIEMEKRNDSIRESPSGTATIKIGDDSNSGEILVF
                     TTLCNVYKVQKKELMDKVQALQKIDAEPGERPVYLTGEKRYKGFLIVAFENGKIGKIS
                     MESYKTEYTRKKLRSAFNGESRLVFIELIEHDIDLVAVSSIKKVVLFNTSLINAVDSR
                     TTKGVQVMKPKDGSVMIKVTRLENTKFADPEYYRKSDSLNAIGFYLKPGDEI"
     gene            complement(21049..22395)
                     /locus_tag="PKK03_05220"
     CDS             complement(21049..22395)
                     /locus_tag="PKK03_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83831.1"
                     /translation="MNYLHCFAITYGVSGAAAAIMLAFGVLLVLLWLTALLLENTLLI
                     TIVLALSALHGLFLVLSWLNPLKARKSSMAIALPAALMFFEGGAAHGQTPEVSPKSPM
                     PDNRFVFPLSLNEEPMERLLVASFKDDPEYEGIEPQLFDNNASGKGLKILMYRKDKKV
                     DVYYEPGVCFDEKTFTLGEGLGYTAETKMSPSQFAISESGVDIDIAFRDKSGRNVKIL
                     IKENTRDVRPFPFLAPVGDNVKDPAKLFAVYMKGFDFVRRKGTTVHVTVGDRELTPAN
                     FPITRDRQKVYLMRYASSLVIGEINASVTKPYVIENISRGVVKTGDMRMLINEEGKVQ
                     SCWIDANSDTIELRFPNGFPNLLTLPQNREVRGSWQYAVSGEELFGGTYSLLRDGSKV
                     CIELDVTKNWKPRNIPFSFRVFTGIVRMFRTWPASYRWNATVDLNGMSTESKWQRK"
     gene            complement(22408..22932)
                     /locus_tag="PKK03_05225"
     CDS             complement(22408..22932)
                     /locus_tag="PKK03_05225"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNX83832.1"
                     /translation="MKTAVVYASKHGTTGFVAQKIRNLLPDDEVVMFNLDRRERIDLR
                     PFGRIIIGSPLYSGTSLPVVRKFIEQNLLDLLQKEVGIFVCCMFHDKADSQIIKGFPE
                     LLRNHARSIRNMGGEYRFEEMNCIERLVVKKISGATGSVSEINARNIELFVRELTVDA
                     SQPCFTEPNSFVKL"
     gene            complement(23089..24210)
                     /locus_tag="PKK03_05230"
     CDS             complement(23089..24210)
                     /locus_tag="PKK03_05230"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX83833.1"
                     /translation="MEPILLFGVFEALIIFLLIILKRKKTVSDYLLSSFFLVYSLNTF
                     LSYLEIYNRTHGFPYPLFFFVSTPFLLLHWPILWLYVKSLTDQHFRFRPVYLLNLLPF
                     VLCIILFTFEYYTKPLNEKIVILQSESFKHQWDYPVVIITMTLSSLVYLSWAAVLVRR
                     YNCRIKVYFSETSRYDLHWLRVLMIASAAIYTVIYLAFAIDLIVPLAPFRWMHQISFL
                     LGAVYIIVLGFFGHRQGNLFSEKTIGINLQEPGTISGITYSLTDKENDFIQNLLKYMN
                     ENKPFLVPEITLAALASELEVTPDYLSGIINNKLGKNFFDFINHYRIEEFKTRSLAPE
                     NKKFTLLSIAYDCGFNSKATFNRVFKNVTGITPGEYASR"
     gene            24200..24538
                     /locus_tag="PKK03_05235"
     CDS             24200..24538
                     /locus_tag="PKK03_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83834.1"
                     /translation="MGSIVSSGSQKGTPNYFFPLRNALSMINNCLKFAKPADLITDSL
                     AGRLPAPDLAGCYGVDTSGRRLLALPSVGTRGLPWGQPEEKNDLFASLKGSFLLPFLL
                     QQQAVDATGQ"
BASE COUNT         6154 a         6040 c         6509 g         5792 t
ORIGIN      
        1 aaaaattatc ttaaatttgc agacaaatca gaaatatcgt ataattgctc taattaaaca
       61 gaaactgatc gcaactaatt aaaacacaaa ttatgaaaaa tttcaccaga ctttttgtca
      121 ttctgctcgc tgctgttctt atgtcaggtt gcagcagtct tgacaagatg aagaaaaatg
      181 ccaatctcgt caagtatgaa atcaccccca aggtgctcga gactcatgca ggcattgttg
      241 ctgcacaggt taaggtaacc tatccggaga agtactttga caagaacacc acgctgaaga
      301 tcacccctgt tctttcatat gaaggcggtg agacggcatt tgatgaactg ctcttcacgc
      361 agggagagaa agttctggcc aataacaagt cggttgcatg gactggcgga actgtaaact
      421 ggtcaggtga tgtaaactat atacctgaga tgaaggtgtc tgagttcctt cttcacgtag
      481 atgctgaaag aaagggcaag actctctcat ttgatcccat caagctggcc gacggcgtaa
      541 ttgcaacttc aacccttgtt gaatatcatg caatgcccat ctcgctgaaa gacaactacc
      601 agcgcattgt tcccgaacag aagattgctg acatacttta cagcatcaac cagtcaaata
      661 tcaggacttc agaactgaag gcagccgata tccaggctct caaggattac attgcccttg
      721 tgatgcagaa tgaaaggatg gaagttaaag gtgttaccaa cagttcttat gcttcacccg
      781 acggtcctct cacccttaac gagaagctct cggaagcaag gggcaaggct gctgaaaagt
      841 atctccagaa ggaatatggc aagatccccg aactggctca gctgttcacc aaacagacca
      901 ctgctgaaga ctgggaaggt ttcaaggctc ttgtacagga gtcgtcaatc gctgacaagg
      961 agctcatcct gagggttctc tcgatgtacc aggatcctgc tgtccgcgag aaggagatca
     1021 agaacatgtc aactgcatat gaatcactcg cagagcagat tcttccccag ctgagaaggt
     1081 caaaactgat cgttgacgtt aacctgatcg gcctcagcga cgaggagatc ctcaccgcca
     1141 tcaagggtga tccgtcagtc ctcagcctcg agcagatgct ctatgctgcc accctgacca
     1201 gtgatcccca ggagatgttg aaatactatc agctggctac cgaaaaggaa cccaagtgct
     1261 tcagggcatg gaacaatgtc ggctgggctt atcttcagat gggcaatgct gatgacgcca
     1321 tggtcgctct tgagaaggcc aaggctctca agaatgatga caacgtgaag aacaacctgg
     1381 gctttgccgc tctcctgaag ggtgatgtca aggcagcttc agagtatttc aactcaatgt
     1441 cagccgccac ccccgaatca aaattcggac ttggcaccat tgcaattcat gatggcaaat
     1501 atgaccaggc agtcaatatg ctcggtgaca cacccacgat gaaccttgca ctggcacagc
     1561 ttctcaaggg tgacgtcaac aaggccaaga caaccatgga tgcagtcaaa ccttgcaaat
     1621 gcggtgctcc ctcatacttt aaggcagtaa tcgcagcacg ccttgacaac aaggatggtg
     1681 tcatcaacgc actgcgcgaa gcaatcggct ataacaccaa atggaagaac tacgctatga
     1741 cagacctcga gtttgccaag tacttcaccg atgatgcatt cattgcagta acgaagtaac
     1801 aaaaccacag ataatgaaaa accccgtccg ccggctggcg gaacggggtt ttttaatagt
     1861 cgattgttac cctttgattc ttcctgccag ttcccacaaa actatccctg cgctgacagc
     1921 cacattgaat gagtgtttgg tgccatactg gggtatctcg atgcatgcat ccgacagatt
     1981 caccacctct tcggtaaccc ctctgatctc atggccaaag accagtgcat acctctttcc
     2041 gggctccgtc gcaaaccggt caagtgatac agctccctcg gcctgctcca ccgaaacaat
     2101 cgcgtaatcc ctttctttaa gcaccctgac tgcttcggcg gaactttcaa agtacttcca
     2161 ttcaactgat tcagtcgccc ccagcgcagt cttgtgtatc tcacggtgag ggggcgtggc
     2221 ggtgataccg caaagccata ctgcctccac cagaaaagcg tcagccgtcc tgaaaattga
     2281 ccccacgttg ttgagactgc gtacattgtc aaggacgatt atgaaaggcg acttctccga
     2341 agccctgaat tccgtgacac tcttccggtt cagttcatcg tttgtcagct tcctcattgg
     2401 taaaaatttt agctaaaata gttataaaaa aagagtaaat tacctacctc ttttactcat
     2461 ggtgccccta taaccatttt cattaacctg aacttgtcat gaacatccac gaataccagg
     2521 gaaaatccat tctgcaggcg catggtgttg ccgtgcagga aggattcgtt gccataacgg
     2581 ccgggcaggc tgtaacggta gccgaagaac tgcgcagcaa attcggtacg gagattttcg
     2641 tcataaaggc gcagatccat gccggaggcc ggggaaaggg agggggagta aagattgcac
     2701 gctcaccgca ggaggcggga gaggtggcct cgcgcatgct ggggatgacc ctggttacac
     2761 cacagacagg gccggcaggc aagaaagtgc acaaggtgct tgttgcacgt gatgtctact
     2821 ataagggaga atcggagata aaggagttct acctgtcact gcttcttgac cgcagaaaat
     2881 cagcatacgt ggttgtctat tcaccggagg gagggatgga catcgaggaa gttgctgcac
     2941 gcaatcctga agcgctcttc accgagacgg ttgactcaac ctgttgcctg catgagtttc
     3001 agatgagaac catagcttcg aacctgggtc tgcacggcaa ggcttaccgc gagatggtaa
     3061 cttttctgaa ggggctctgg gaggctttcg tcaattctga cgcctccctg ttggagatca
     3121 atccgctgct taaaaccagt gatgacaggg tgctggcagt agactgcaag gtgacccttg
     3181 acgacaattc attataccgt cacccggagc tggcggccat gcgtgacctg aacgaggagg
     3241 atcccaccga ggtggaagcc tcagccagta acctcaatta tgtaaaactc gacggtgatg
     3301 ttggatgcat ggtcaatggc gccggcctgg ccatggccac gatggacctg atacagctct
     3361 ccggcggcaa gcctgccaat ttcctcgatg tgggcggtgg cgcgagcccg aaaacggtgg
     3421 aggccggatt cagaatcatc ctcaaggacc cgcatgtgag ggctatcctc gtgaatattt
     3481 tcggcggaat cgtcaggtgt gaccgcgttg ccgcgggaat tattgaagca tacaagagca
     3541 tcggcgacat aaaggtgcct gtcattgtca ggctccaggg aaccaatgca gaggaggcca
     3601 gggcgctgat agacagctcc gggctgaagg tcattgccgc ctcaacattc accgatgctg
     3661 ccgaaagggt cagggctgcc ctgagctgat tcaggcattg ccctcccgtt cataaattca
     3721 ggccggagac ttggcctttg ttggcgttta ttcttaattt gccgccgttt caaccaatga
     3781 gcagacagtt cgcagatata atcgttcccg ttgccgtacc cggctcattc acctatgaga
     3841 ttccggagca gcttcagggt acggttcgcc ggggcagcct ggtgaacgtc cccttcggtc
     3901 agagcaggaa gacggccggt ctggtggtga aagttcatga catccctccg gcagaagtct
     3961 ctctcaggga ggtagacagc ctcctcccag gcgaaacggc tgtcaatgac cgtctcgccg
     4021 acttccttct gtggatatct gattattaca tggcctatcc gggcgaggtg atgaaggcgg
     4081 ccataccgtc gcctgatgat cttcccgggc gcgtgatgaa gcgcaggccg aaggaggcgg
     4141 ctgatcttcc ggagaccgag atggtcatgc cggcagagct gaacagggtg cagaaggagg
     4201 cctatgattc tgtcaaacag ttgtttgccg atcgtgagac ggtgctgttg catggcgtta
     4261 cctccagtgg caagaccgag atctatattc accttatgcg tgagcagctt gaccagggaa
     4321 ggcaggtcct ttacatgctt cctgagatag ctctcacaac gcagatcatc gagaggctca
     4381 ggaaacattt cggcagcgcc atcggggtgt ttcactcgcg tctcacccct gcggcgcgaa
     4441 gaaaagtatg gcagaaggtc agtgacggaa gcctcagggc tgttctgggg gtacggtcat
     4501 cgctcttcct tcctttcagt cacctggggc tgatcatagt ggatgaggag catgattcat
     4561 cgtacaagca gaatgatccg gcccctcggt atcatgcacg tgatgctgca atgatgcttg
     4621 cccgcatcca cggcggaagg gcgctcctcg gttcggccac gccatcgctt gaaagttacc
     4681 acaatgcact ttccggaagg tacgggtttg ttgagctgtt gacacggtac ggagaggtca
     4741 tgatgcctga gatggtcatc gctgacactc gccggaaagg caaaaagaag ggaccatcag
     4801 ctcatttcac cccccgtctt cttgaggcag tggaggaggc gctgaagcgc gaagaacagg
     4861 ttatcctctt ccagaacagg agaggtttct cgcccttcct gatgtgctct gattgcggac
     4921 atgtccctgc ctgcaccaac tgttcggtga gctataccta tcacaagagc atcaaccggc
     4981 tcgtatgcca ctactgcggc aagagcgtga gactaccctc agagtgcccc gattgcggat
     5041 cagtgaacat ttccaccatg ggcttcggca ccgaaaaaat cgaagaggaa ataagactcc
     5101 tgttcccttc ggcgcgggtg gcgcgaatgg accaggacac cgtcaggaac aggggcgctg
     5161 cctctgacat actgggcagc ttcgccgcgg gcgataccga tatccttatc gggacacaga
     5221 tgatctccaa gggactcgac ttcgagaggc tgacggtggt gggcatactc gatgccgaca
     5281 atatgatgca tttccccgat ttccgggctc acgagcgcag cttccagctc atggaacagg
     5341 tgagcgggag agcaggcagg aggacacgcc ggggaaaggt gattatccag accgctgacc
     5401 ctgcaaacat gatcctcagg caggtcctga aacatgatta caggtcaatg agctcggcac
     5461 agctcgggga gagggctctt ttcggctatc cgccctttac aaggattata aggatagcgc
     5521 tgaaacaccg tgaccttgat gaacttactg cctcggccgc aaggctggct gatgccctcc
     5581 gcaggcacct cggcaaccat gtactgggtc cggagtttcc gatggtgatg caggttcaga
     5641 agtggtacat caaaacggtg atgataaaaa tagtcagcac actctccccc tcgaaagtca
     5701 aggagctggt gagacgcgca atggatacag aactgaaatc tcccaggaaa gggctgctgc
     5761 gcattcatgc cgatgtcgat ccacagtgac ctcagttgcc ctttaatatt aaaaggttat
     5821 ttttacggat tcaaagctgt tttaatgaaa atagaggttt caaacggaga gattgcggac
     5881 aagctgacga ttattgaact caagctggag cggatcagtg atcccgaaaa actgaagaac
     5941 ctgaaaaagg agcatgccat tcttgatgag gcgctcgcgg gtttcatgaa gaaggatgac
     6001 ccgctgtacc tggagctcta ccgcgtcaac gccggattgt gggagattga ggaccggatc
     6061 agggaccttg aaagaaaaaa ggattttgga gaagaattca ttgagactgc caggtcagta
     6121 tacttttcaa atgacctgcg ttccgaaatc aagcggaaga ttaaccagat aaccggttcg
     6181 ggtctcgtgg aggaaaagtc gtacgaaaaa tactgagatg agccttcttg tgatacgcac
     6241 atcatcaatg ggtgatgtga ttatgaccct gcccgtgctc aggtcgtttg ccgctgcata
     6301 ccctggcaaa gagctggttt ttgtcaccaa gaagcctttt gatcagtttg tcaccggcat
     6361 tcccggggtg aaggtttttc tttccgatca tcacggcagg cacaagggtt tccggggcct
     6421 gctgagggta tggaaggatc tacggtcaat gtaccacatc actgcggtgg ctgacctgca
     6481 tgacgttatc aggtcgaagg tagtccgcct gttgtttcgt atgggcggct gccacgtggc
     6541 ctcgatagac aaggagcgta tccgccggcg caagctggtt cgcggtaggc tcaccggctc
     6601 gctgacccac accgttgagc gctaccgcaa agtatttgag cgtgccgggt atccgctgga
     6661 gctggttccc ggcccctgga tgttcccgtc tgctgaagag agagcggtgg cagccgcccg
     6721 taccgcgggt atgaaaagac ctttcctggg tgtggcgccg ctggcgagac accgtctcaa
     6781 gacatggccc ccgggaaaaa tggaggctat gctccgaatc ctgatatcgc ggaaggaatg
     6841 cacattattc ctttttggtt ctcctgatga atccgctgca gtggatgaga tagctgcccg
     6901 cgtgccggga gccatatcca tggcagggaa gatcagcctt gaaggcgaga ttgcacttat
     6961 cgcagacctt gacctgatgg tggccatgga ctcgtcaaac atgcacctgg cttccatgct
     7021 gggcactgat acactgtcaa tctggggcgc tacccatccc cgtgccggat tcagtgcctg
     7081 gggaatgccc cctgagaacc agatccagat acctgacacc gaacttacat gccgcccctg
     7141 caccatctac ggtaagggga cctgcagacg cggcgacctg gcctgcctgg agtggctgac
     7201 accggagatg gtgcttcaaa aaatcctgaa caaactgaag tgacatatga aattcaccgg
     7261 aaccaaccat ctgatcattg ctttagcttt cataaccctt gccggcgccg ggtgcagaca
     7321 gacgcccgaa ggcactgctg accgtatttc cgcagaaccc gctgacctgg taggcacact
     7381 tatgggtaca gattccgagt atctgctatc ccacggcaac acctacccgg cggtagcatt
     7441 gccgttcggg atgaacttct ggaccccgca gacccgcgag aacggcaacg gctggggata
     7501 cacctgggat gaccataaga tagcaggtat taagcagact catcagccaa gcccctggat
     7561 aaatgattat gcagccttct cgctgatggc tgtcacgggt ggtaaccgcc tcacggaaca
     7621 ggcacgctca tcatggttct cgcataaggc tgaggtggca aaaccctggt attacagcgt
     7681 ttacctcgct gactttgaca tcaccgcaga ggtgacaccc accgagaggg cagctctctt
     7741 cagatttaca ttccccggga gcgactcctc cgccgtggtc attgatgcct tcgccggcgg
     7801 atcaatggta agaatcattc ccggaagcaa cagggttgtg ggctactgcc gcaacaacca
     7861 cgggggagtg cctgaaaact tccataatta tttcgtcatt gagtttgacc ggccgttcga
     7921 cttctggtcg gcggcccttg acggcagtct ccggcccggc gtcaccgaga ccgagggtga
     7981 ccgcgcccag gcggtaatag gattccggac aaaaagaggt cagcaggtca ccgccagggt
     8041 ggcttcatca ttcatctccc cggaacaggc cattctcaat cttgaacgcg agacgggcgg
     8101 gcgcggattt gaagctgtcc gtgacgccgg acgtgaacgc tggaacaccg aactgggccg
     8161 cataaaggtg gagggcggca ctgatgacca gcagcgtacc ttctattcaa ccctctaccg
     8221 caccatgctc ttcccccgga tgttctacga gactgacagc tccggaaata ttgttcacta
     8281 cagtccttat aacggcaagg tgcttccggg cagactcttt accgacaatg gcttctggga
     8341 taccttccgt gccgcaatgc cgcttatcac tctcatgtac cccgagatga gctcgcacat
     8401 catggagggt ctggtcaata ccttcctgga gagcggctgg ctgcccgagt gggccagccc
     8461 gggccaccgc aactgcatga taggcagcaa ctccgcctca ctgatagctg atgcatacct
     8521 gaagggaata aggggatatg acatcgacac tctgtggaag gcgatggtga agaacaccct
     8581 cggtcacggc ccggtgcatt ccgtcggcag gtatggggct gaatactaca acaggctggg
     8641 ttatgtgccc tacgatgccg gcataaatga gaatgtggcg cgtaccctgg agtacgccta
     8701 cgcagactgg tgcctgtgga agctggcaca ggagctgggc cggccgaagg aagagattga
     8761 cctttacgga gcccgggccc ggaactataa aaacgtcttt gacgttgaga gcaggctgat
     8821 gaggggcaaa aacctcgacg gcacgttcca gtcgccgttc agcccttata aatggggtga
     8881 tgccttcact gaaggaaaca gctggcatta cacctggagt gtctttcagg atgtggccgg
     8941 tctggcagag ctgatgggcg gtgacaggga gatggcacgc atgctcgatt cagtatttgt
     9001 ggtgccgcca ttgtttgatg actcctatta tggcttcccc attcatgaga tacgtgagat
     9061 gcagataatg aacatgggca actatgccca cggcaaccag ccggttcagc atatgatcta
     9121 cctctacaac tacacgcctg agccatggaa ggcacagatg agggtacgcg aggttatgga
     9181 taagctttat aactacaccc ctgacggtta ctgcggagat gaagataacg ggcagacctc
     9241 ggcctggtat gttttcagcg cactgggttt ctacccggtg acgcccggaa caggcgaata
     9301 tgtgcttggc tcgccgctgt ttaccagggc gacggtcacc ctctctgacg gcaggaagct
     9361 ggtaatcagt gctccggaga acagccacga taatatattc gtaagagatg taacctttaa
     9421 tggcaaaaaa ctgaacctca attttgtgac ccatgaacaa ctcactgcag gaggcaccct
     9481 ggagtttgac atgagcagtg aacctgacag ggagaggggt acggcacctt cagccagacc
     9541 atattcaatg agcacacatg gcatacaaaa ctgatttttc atattaataa ttatggcttc
     9601 actgaattta aggggtattt ttccgcccct tccgacatca tttgacagcg acgagaacct
     9661 gctgccggaa aagatcaggt ataacattga aaggctttct cagtatgacc tggccggttt
     9721 tctgatcctt ggctcaaacg gggagcaggt gatgctctcc gaacccgaaa agatcaaagc
     9781 catagcagcc gcccgggagg cgcttccgga cggccggatc ctccttgcag gcaccggatg
     9841 ccagagcacc cgcgagacca tttccttaac cagggcagct gcagctgaag gggctcatgc
     9901 cgtgctggtt atcaacccct cgtattacag gggaaacatg aatcatgatg agttggtgaa
     9961 tcactatttt gatgtggcag atgcttcacc tgtgcctgtt cttgtttaca acatgcccgc
    10021 caacaccggg attgacatga ctgccggaat gatcatctcc gtggcagagc acccgaatat
    10081 tgcgggactg aaggagtcgg gaggaaatgt tgtaaagatg ggagaggtgc ttggcagggt
    10141 taagcccgga ttccaggttc tttccggcgg tgcagggttc ctgttaccgg cgctggctac
    10201 gggcgcatca ggcggcatcc tggcaagcgc caacatcgcc cctggtcact gtctgagcat
    10261 cttcaacgct ttcgccgcag gcgatatgac acttgcaagg gaactgcagc acagcatcat
    10321 ccctctcaat gcagctgtga cggcacgctg gggcgtgccg gccctgaagg ctgcaatgga
    10381 tcatctcggc ctctatgggg gccatgtaag gagaccgttg ctgccgctca gggaagaaat
    10441 acggaagcaa ttgctgcaac ttttggatga caggaatctg aatctgtttg gtgagaagaa
    10501 ataagagaaa atgaaaggca gaaaattatt gatgataccc ggcccgacag agttcgaacc
    10561 ggaggtgatg atggctgtcg gagcgcccac cacgagtcat gtggcgcctg attttattga
    10621 atcgttcggt catgcacttg agatgatgag ggaggtttgg cttgctcctt cagggcagcc
    10681 ttttatcatt gcaggcagcg gcacactggc gatggatctg gccggtgcaa acctggtgga
    10741 gccgggcgac agggcgctag tgatctccac cggctacttc ggcgacaggt acgcagagct
    10801 cctgaaacga tacggtgcag aggtgacagc gctgaaggcc cctgcgggtg aagtcgtcag
    10861 tgatgcagct gtggagacgg agctgaaacg taagcggtat aaactgatga ccttcactca
    10921 cgtggataca tccactgctg tacgggttga tccggagccc ctggggctgc tcggagaaaa
    10981 gcatggagta ataacggtgc ttgacggcgt ctgctctgtg gcaggcgagg tgataagaca
    11041 agacgaatgg aagattgatg ttgtatttac cgcctcgcag aaggctgtag gggtgccgcc
    11101 cggactggct ttgctggttg cttccccgcg ggcaatggag gcatggaagg gaagaaaaag
    11161 cccggtggga agctattaca gcgactggac ccactggctg ccggtgatga aggcatacga
    11221 ggaacggcgt ccggcttact tcggtactcc ggcggtgaac ctggtgtcag ccctcgaggt
    11281 gagcctcagg ctgatattga aggaagggat ggaggcccgc ttcagaaggc acagtgaccg
    11341 gggcaccgcc ttccgcaaag cgatgcgtgg catgggactc accatgatac ctgccagcga
    11401 ggaggtcagc gcaaacaccc tctcagcacc attatacccg gaaggagtga aaggagctga
    11461 tttcatgaaa gctatcggcg attcggacgt gatcgtggca ggagggctgc ttcccggtat
    11521 aaaggataca tatttcaggg tcggccacat gggagcagtc accaacggcg atatcctgac
    11581 aacggtggct gccattgacg gtgccctttc ggcctgcggc tacaggaaat agcgtaaatt
    11641 tgtaacattc atacacgtaa taataccaat cagcaacatg tcactcacag aaaagattgc
    11701 aaaggacctg atggcagcca tgaaggctca ggacaagggg atacttgaag ccctgagggc
    11761 ggccaagacc gcgtttatcc ttgccaggtc agagagaggg caggataccg ttctcaccga
    11821 agacgaggag gtaaagatca tccgtaagct ggtgaagcag aggaaggaat cggctgacat
    11881 atatcgcgag cagaagaggc ctgacctctg tgagaaggaa gagaacgaag ccgcagtgct
    11941 tgaaagatat cttcccgcag caatgacaga gcagcagttg gagattgccc tccgggagat
    12001 catcagccgt gtgggggcga agggcccgtc agacatgggg cgtgttatgg gtgctgccac
    12061 caaggaactg gcaggaaagg ctgaaggccg tgagatttca gcaaaagtga agcaactgct
    12121 gggataaggc ggcagggccg gtcatgagcc gggggctgta tccgccttct tgtcattctc
    12181 aagcattcgc aggatgcggc ggcgctttgc aatcaggtca gtcagctcga taagcatgtc
    12241 ggtgttgacc ctccgggcca tcggaatgac atatttctct gtctggtcaa gatagtccga
    12301 gattttctgt tccgactgta tatcgcggtg caggtctgta aggttgctga tccggtcagc
    12361 gcacttgagc agcatggcat tccgcgatcc gtattcaagg aggcgctgca ggtactgctc
    12421 tttcgtctca tccttcctct tggtaacctc cagcacaagc tgcacaacat cggcaccatc
    12481 actgtcaatc caccttatct cgtcaacctg ggtctcagga agatcctcaa gcagatcatg
    12541 cagcagggag gccttgagca agatgctgtc agtgaagaac ttgtagtcaa gcaatatccc
    12601 gagcgtcgaa aaacaatgcc tgaactggtt gccgcccaca gccctttgca cgcttataag
    12661 agcggtggcc ttaagaatat aaggcgccag cacaaggttc ttcagtgtct caagggcagt
    12721 catggtaaca ggttgcaaaa ccataaaatt aatgattccg gagattgcca ccggttatga
    12781 tgtcatacta attataaaca gcctcttaac aatattaaca cctgtgagga atagattgtt
    12841 aacgatgatg agatgatatc ggtgaacatt atcccctata tttgcatttc tgttgatgaa
    12901 ggagtgaaag atgccggata aaccatccag accgtcagga tcaggattga agcagatggc
    12961 aaggctttac aggtttctga agccttacag gggcaggttt gcccttggcc tcgtatttct
    13021 tcttctatcc acggcagcca gccttatgtt cccccgtctg ctcggcgaaa tggttgacca
    13081 ggccaacagc ggaggactta cacgcgagat aacacgcaca ggactactcc tccttgcaat
    13141 actggttgcc cagtcgcttt ttgcctatac ccgcacacgg ctctttgtgg tggtgactga
    13201 aaagaccctg gcatcaatac gtcagcatat ttataaccac ctgataagac ttcccatgtc
    13261 tttcttttca tcaaggaggg tgggagagct caacagccgc atatcatcag acatatcgct
    13321 gctgcaggac agccttacca ataccctggc agatctcctt tcacagctgc ttgtgatcac
    13381 cggtggtatt accctgatga tgatcagctc attcaggctg acgattttca cactggcaat
    13441 tgtgccggct atctcgctga tggcgtttta ctccgggagg gccatcaggc gctattcaaa
    13501 gaaagcacag gctttcgtgg cagagtcaaa caccatagtg gaggagactt tgcaggggat
    13561 acagaacgtt aaggccttca ccaatgagtc atttgagagc cgccggtatc gtgagaagac
    13621 ggagcaggtg gccagggcag gcattaccgg cggaaagtac caggctgcca tctcatttat
    13681 agttctgggg ttctttgttt ccatggcggc tgtcatctgg cagggtgcca ccatgattgc
    13741 aaagggtcag atggaagccg gacagctttt cacctttgta atctattcag gattcattgg
    13801 cggcaatatc gccgggatgg cgggagtcta tgcaagacta cagaaaacca taggcgcttc
    13861 ggagaacctg ctgcaactgc ttgatgaaaa gactgaagaa atagatcctt catttattcc
    13921 cggtgaagcc caccttctga atggtgagat aacctttgag ggagtagggt tcaggtatcc
    13981 gtcgcgcaat gaagtggtgg tcctgagaga tgtcagcttc gaggttgctc ccggcaggca
    14041 ggtggcactg gttgggccca gcggtgccgg caaatctacg atagcttcac ttcttctgag
    14101 gctttatgac cccactgagg gaaggattct gtttgacggc cgcgacagca caggcttccc
    14161 cctgacagcc ctgcggtcac agatggcagt ggtgatgcag gatgtgtttc tcttcggagg
    14221 caccatcagg gaaaatattg cctatggaaa accgggcgct actgaagatg aggttattga
    14281 tgctgcgaaa caggccaacg cgtgggactt catcaggaca ttccccgggc aacttgagac
    14341 agtggtggga gagagaggtg tgcagctctc gggcggacag aggcagagga ttgccattgc
    14401 acgtgcggtg ctgaagaacc cgcggatact gatacttgac gaggccacct cgtcactcga
    14461 ttccgaatca gaacgacagg tgcaggaggc gcttgaaaag ctgatgaagg gacgcacatc
    14521 actcgtaata gcacacaggc tctccactgt acgcaactca gatcacatca tagtgctgaa
    14581 tgatggaagg attgttgagc aggggaccca cacagacctg atctccaacg gaaacgggct
    14641 ttacaaaaca cttaccgagc tgcagttcag cctgtgagtc acgcctgtct ggtgtgacag
    14701 cggtgttgcg ctgattgcct tccgggatga cagataacac tcagtggctg ttcctgcttt
    14761 tcagggatac agaagcctga ttgtccaccc ctccctgtcg ctgttccttg tgtagatcag
    14821 cctgtcatgc aggcggtgtt cccttccctg ccagaattcc acataatcag ggtcaaggca
    14881 gtatccgccc cagtcgtcag gccttggtat gggcctgtcc ctgtattttt cctctgtctc
    14941 cgcagcagtt ctgtcaagca gttccctgct ctccaggtgc ctgctctgct gtgatgccac
    15001 ggcgctgatc tggctctcgc gcggtcggct gcggaagtag gcatctgatt catccgacgg
    15061 caaccttgac actcttccgc ctatccggat ctgcctgtag agctcattcc agaagaaggt
    15121 cattgcagca tggctgttgt ttctcagctc atcccccttg cggctcccgt agttcgtgaa
    15181 gaaggtaaat cccttcgggc tgaagtccct tagaagcacg atccttcctg acggccttcc
    15241 ttcagcatct gccgtggcga ggtgtacggc attgggatcc attaccttat acgagatggc
    15301 atcttccatc catcgcccga actggaccac cgggtcatgt gagaaatcgg cgaggtttaa
    15361 accctcacca tgatactcat ttctgagaag agtcgtcatt ctgcggattg aattcataag
    15421 atcagaagat tttattactg aaaagaaaca cctgtctgtt gattatgttt attttgcatt
    15481 gtcggttaca ttggattgaa aatactacat ttgttttaga aaaacataaa cgtatgtctt
    15541 tgacgcgcat tctgccaaca tttgctttga ttataaccct gactgtcccc tcagtcgtca
    15601 gtgcgcaggt acctgtggag gtgtcaactg agaaagtggt ctccggcgga agggtttatt
    15661 acatgcatga ggttcagaag aaccagactc tctattccat tgcgaaggcc tacagagtat
    15721 ccgttgatgc cctgtcacgt gagaatgtca tccctccaaa cgggatccag acggggcagg
    15781 tgctgagggt acccgcaccc gatcagcagg gttcagctgc gtcgcagccc cgtctgccgg
    15841 ctcagcaggc tgctgctgtc acccagcccc gtcccgcagc gcagccttca cgacctgcgc
    15901 ttaccagcgg tgttcccggg atagatattt caggtgaaaa gatcatatcg ggcggaaaga
    15961 cttttttcat gcatgaggta cagaagggcc agactcttta ttcgattgca aaggcctaca
    16021 gggttaccat tctggatatt gatcgtgaga actctatacc gccgggaggg attcaggcgg
    16081 gccaggttct gaagattccg gcatcatcgg cgctaatggt gtctgaggag cagacacctc
    16141 cttcagccgc tgctgcaact cctgacagga gcaccgcagc tgcggcaaat gcatccgggg
    16201 gtaataccgc tgcagtaaca aacatgccaa ggcaggagac tccccgccag gagacccagc
    16261 tgccaaggca ggagactccc cgccaggaga cccagctgcc aaggcaggag actccnnnnn
    16321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacctc accttcatca tgattgtatt
    16441 tcactgcatt tgagacaaga ttgctgatta tgatctcgat ctcgttacgg tcagccctga
    16501 tgaagcagtt ttcgggaatg tcggcatgaa tctttacctt cttttgtttc gcaagaggct
    16561 caatggtgtc gattacgctt ttagtcacct ggtggaggtc aacattggta atgtggcggt
    16621 ttttttcccc ggcctcgatt ttggtaaggt ctatcaggtc ggttatgagg gctctcatcc
    16681 cgcgcaggcg ctccttgcat ctctcaagca tcaccatgta atcatcaacc gagtcgccaa
    16741 cctgtttttc aagaatgatg ttcaggtacc cttccactgc attgattggc gatttcagtt
    16801 catgtgacag cacggagagg aactggaact ggctctgcct gccctcttca gtcatgcggg
    16861 tggtcatacg cctcaggaag aggtttttgg taatggcttc cactgcggtt ctcagttcct
    16921 ggggggtgaa gggtttgggc atgaagctgt gcgctccgat ggtggtggct ttgacggcca
    16981 ggtcaagtga agcgtatgag gttatcatca taactgcaat gtcatgcttc ttctccctga
    17041 tgtattccag cacctcgatg ccgtccatcc cgggcagctt gttgtcaagg aggaggatat
    17101 cgacgggggt actgtcaata atacggagag cctcttctcc gctctctgca gtcataatct
    17161 catatgtgaa gtcttcatcg aggaacgggt aactcacccg gaagttcctc atcgccctct
    17221 cggtgcctga tctgatgccg ggttcgtcat caacaaccag tagtttcagt acgctcatga
    17281 acagataaag attaacagga gttcatgaat ttactaaaca taaaccggaa tcacaaatgg
    17341 tgcgaagtca gtttccggct ttcctgttcc tttttcgctc ctgcctccgg agtatcagga
    17401 atgttgtcag tccggcaacg gatgacacga tcagtgccat gacaagactg ccggtaacga
    17461 attgaagcat cccttcagtg atgtctctga agtctttgat actgttataa tccgggtcaa
    17521 ttgccccgct gccgaggatt aatcctccga gccggtaact tccgtagata atgacgggga
    17581 tcagaggggg caggcttatg gcactggaac ctatggtcag tatcttgtta agcctgaaaa
    17641 gataactcgc tgcaattgca gtcaccgtct gaaatcccca tacgggagac aggccaatga
    17701 atacacctgt tgcaacggac agtgtcttgc acctgacagt gcccgaggtt gagaataact
    17761 cagccactac cttcgtgagg ttttccttca ggctgctcct gtttcccttt tcattcatga
    17821 gccgggaatt ttaagtgctt cacctgaaaa ccgcggaggt gttaatctga tccttctcaa
    17881 gaggtctgtt atggatttaa ataaattctc cttgaaattg tcctgtcttc cctattaaga
    17941 gcattgtaca aatataaatc aatctgcagg ttcaggcaat cgcctatttg gtaccggttc
    18001 ctgcattcaa tccacaggac aaggcacctg ccgctttcat tgacgaaagt gccggagagg
    18061 ctgcagatga cagtgtgcgg actgtcgggc agccctttca ggctgccccg ggtccgcact
    18121 tcgctttcct gacggggatc agatatcagc ttgccggtct ctgatgacaa cgccaacagg
    18181 aaagcaactg ctaaaaaggt gattgcaaca ttcttcacgg ctgccggcat ttatttgttc
    18241 ccggtgacaa caaactcgca tttgtcgctt tcccacaggg tggatctgac gttgaggcgg
    18301 tagcctcttt caaggccttc tacctgtgcc ctgacgggat tgatacagta gatgttgcag
    18361 ggtttgggca ggttcatctt cttcgcgata gcgcagagga ggcatgagtg acctctggct
    18421 gtgagcttct cctcagtctc ctcgatgttc catgagatgc agccaaacac ctctgagaag
    18481 acattgaata ttccggacgg gtcggtgatc cggagctgtg ccagctgacc cttcgcggcc
    18541 attttctgtt ccttttcctt ctgagcttca acgtcggcaa ggatgccggc ttcggagtag
    18601 cgtctcactg aatctgcaag tactccggca tagaagagct gcagcatctt taatcttttt
    18661 tcggtttcca tttttttatg gttaagtttg ttatgcaaag tacgggcggc ccggaatgct
    18721 gctcaatagt gatcattcat catttttatg gacgtacagt cactgcagaa caggatgacc
    18781 aggcttctcg acagccagaa gccctcccct gaacacacag attattccat actgaactac
    18841 catatgcggt tgcttgagca gatagccctc atggagaaca gctcaatgac gatatacgat
    18901 attcttcaga agaggtacgt ctttgtccgt aaccgtttcc gtgagctgat tgactatgac
    18961 gagcaggctg ccgccgaaca gggttatgcc ttttttttca ggctgatgca cccggatgac
    19021 atctcgtttg tgcttgacac aagcatcagg gcaattgagc atattatagg gctgcctcca
    19081 gcagaacgaa aggattataa gaccattttc gagttcaggc tgaggaacag ggaagggaag
    19141 tatatcaggt tcatacagca gctggtaaac ctggagctgg atctgcaggg gaatatgtgg
    19201 ctgatactga tcgtgatgga tgttaacccg aaccagcagg gcagcaagca gatgttgcgc
    19261 agtacggtga acatgaagac agggaaggtg gtgtctttca ccgaggaagt tgaagagagg
    19321 cagtcgcagc tcaccaagcg ggagattgag atcctcggtt tgcttgccag gggcatggtc
    19381 agcaaggaga ttgccgacga gctattcatc agtgtcaaca cggtgaataa tcaccgccag
    19441 aggatcattg agaaaatgga tgtggccaat acctcggagg cgctgaacta tgcaacgagg
    19501 ataggaataa tttaatagaa agatatgtga tgagtgactt tcagatgttg attgacgagc
    19561 tggttaaatt cagggatgca cggcaatggg agcagtttca caacacaaag gatctggccc
    19621 tggccttgtc aattgagaca gccgagctga atgagctctt tctgtggaaa ccggttgaca
    19681 aatcaaagga tactgcaagg aagtacaatg agttgtgatt gttttcagaa tggttggcat
    19741 acctcagtaa actcatatga ttgaatgatg ataaaactgt tacttggcga cattacaagg
    19801 atcgaagttg atgcaattgt caacgcagca aattcgtcgc ttctcggcgg gggtggggtg
    19861 gacggcgcaa tccacagggc aggaggcagg gccatcctgg acgaatgcag gaggattgtt
    19921 gaaagacagg gcggttgccg gactggtgag gcggtgataa ccacggccgg gaagctgccg
    19981 gccaggtatg tcatccatac ggtgggtccg gtatggcacg gcggaattaa caatgaggat
    20041 cagctgctga cgaacgccta ttccaactca ttgcagcttg cagcggataa cggagtaaaa
    20101 actattgcat ttcccaatat cagcaccgga gtctacggct tcccgaaaga gagagcagca
    20161 aagattgcaa tcgaagccgt caggaagttt ttgaaaaatg acaaatcgct gcgtgaagtg
    20221 gtgttcgtct gttttgaccg ggaaaaccat gaaatctatg agaggctgct gaatgcctga
    20281 gcattgattt tacagttaat tataacagcg aaaaatccta tggaattatg cagatgcaaa
    20341 ttgaataatt cccacatttg ggttcaaatc aaaacctgat taccatgcaa agtgcagaaa
    20401 gcctgagaag gaaaatagag atggaaaaga ggaatgattc aataagggaa tctccctccg
    20461 gaacagcaac cataaaaatc ggggacgatt caaattcagg agagatcctt gtctttacca
    20521 ccctgtgcaa tgtttataag gttcagaaaa aggagctaat ggacaaagta caggcgcttc
    20581 agaagattga tgctgaaccg ggtgaaagac ctgtatatct gaccggagaa aagagatata
    20641 aaggctttct gatagtcgca tttgagaatg gcaaaatcgg gaagatctca atggaaagct
    20701 ataaaaccga gtacacaagg aagaaactca gaagtgcctt caatggcgaa tcgcgcctgg
    20761 tttttattga actcatcgag catgatattg acctggttgc cgtgagcagc attaaaaagg
    20821 ttgtcctgtt caacacctcc ctgatcaatg cggtggatag caggacaaca aaaggagtac
    20881 aggttatgaa acctaaggat ggcagcgtta tgataaaggt caccaggctg gagaatacga
    20941 agtttgctga tcctgaatat taccgtaaga gtgactccct gaatgctatc ggattctacc
    21001 tgaagccggg agatgagatc tgatacctgt cccccttccg gctttctgct atttcctttg
    21061 ccatttgctt tcggttgaca tcccgttcag atcaacggtt gcattccacc tgtaagaagc
    21121 cggccatgtc cggaacatcc ggacgatgcc ggtaaatacc ctgaaactga acgggatatt
    21181 gcgaggcttc cagttttttg tgacatcgag ctcaatacat accttgctgc catctctcag
    21241 aagtgaatat gtacctccga aaagctcttc gccggagacg gcatactgcc aggaacccct
    21301 gacttccctg ttctgtggta atgtcagaag atttggaaaa ccgttcggga atctcagctc
    21361 aattgtgtca gaattcgcgt cgatccaaca gctctgcacc ttaccctcct cattgatcag
    21421 cattctcata tccccggttt taactactcc ccgtgaaata ttttcaatga cataaggttt
    21481 tgttacagat gcatttattt caccgatgac aaggctggat gcataacgca tgaggtacac
    21541 tttttgcctg tctctcgtga ttggaaaatt tgcgggtgtc agttccctgt cgcctactgt
    21601 aacatgtacg gttgttcctt ttcttctcac gaaatcgaat cccttcatat ataccgcgaa
    21661 aagcttagcg ggatccttta cattgtcgcc cacgggagca agaaacggaa aggggcgcac
    21721 atcccgggtg ttttctttta tcaggatttt tacattacgg cccgatttat ccctgaaggc
    21781 aatatcgata tcaactccgc tttctgatat ggcaaattgt gacggtgaca tcttggtctc
    21841 agccgtataa ccaaggccct cgccaagggt gaatgtcttt tcatcaaagc agacccccgg
    21901 ttcatagtac acatcgacct tcttgtcttt tctgtacata agaatcttta agcctttccc
    21961 cgaagcattg ttgtcaaaca attgcggttc aatcccctcg tactcgggat catctttaaa
    22021 actggcaaca agcagacgtt ccatcggttc ctcatttaag ctcagcggaa aaacgaacct
    22081 gttgtcaggc atggggctct taggactaac ctcaggagtc tgaccatgcg ctgccccgcc
    22141 ctcaaaaaac ataagagctg caggcagagc aatagccatt gatgatttcc tcgccttaag
    22201 cggatttaac cacgagagaa ccagaaaaag accgtgcaaa gcagaaagtg caaggacaat
    22261 ggtaataaga agggtgtttt ccagtaataa ggctgtcagc cataaaagga caagcaagac
    22321 tccgaaagcc agcatgatgg ctgctgctgc tccggacact ccgtaagtga ttgcaaaaca
    22381 atgtaagtag ttcatcgtaa ttcagtttta aagttttaca aatgaatttg gttcagtgaa
    22441 gcagggttgt gaagcatcaa cagtcaattc cctgacgaac agttcaatgt tcctggcatt
    22501 tatctcggaa acactgcctg ttgccccgga gattttcttc accacaaggc gttcgataca
    22561 gttcatctct tcaaaccggt attcacctcc catgtttctg atgctgcggg catgattcct
    22621 gagcaattcc gggaatcctt tgataatctg actgtcagct ttatcatgga acatgcagca
    22681 gacaaatatt ccgacttcct tctgaagcag gtcaaggaga ttctgttcaa taaactttct
    22741 gacaaccggc aacgacgtac ctgaatacag tgggcttcct ataataatgc gcccgaatgg
    22801 acggagatcg atccgctccc gcctgtcgag gttaaacatg accacctcat cgtcaggcag
    22861 aagattcctt atcttctgtg ccacgaaacc ggttgtgcca tgtttggatg cataaacaac
    22921 tgctgttttc atttctttgt gttttgagat tatggcacaa aggtagatga ggcccgaatg
    22981 acgggggtct caacaagttc tgaggaggtc tcggaaaacg gcttttatgg ggaacagagg
    23041 tctcagctta atgagactca cggtattttt ctgaaaagga gagcagattt accggctggc
    23101 atattcaccc ggagtaatgc cggtgacatt cttaaaaacg cggttaaatg tggcttttga
    23161 attgaatccg cagtcgtagg caattgagag aagggtgaat tttttgtttt cgggtgccag
    23221 ggaccgggtt ttaaattcct cgatgcgata gtgattgatg aaatcaaaga aattctttcc
    23281 aagtttattg tttatgattc cggacaggta atcgggcgta acttccagtt cagaagccag
    23341 ggctgcaagg gtaatttccg ggaccaggaa aggtttgttt tcattcatat atttcagcag
    23401 gttctgaata aaatcatttt ccttgtcggt taatgaatag gttatgcctg atatcgtacc
    23461 gggttcctga aggtttatgc caattgtttt ttccgaaaac aggttgccct gacggtgtcc
    23521 gaagaatccg aggacaatga tataaactgc acccagcaga aagcttatct ggtgcatcca
    23581 tctgaacggg gccaggggaa ctataagatc aattgcgaaa gccaggtaaa tgacagtgta
    23641 gatcgcagct gaagctatca tcagcacgcg aagccagtgc aggtcatacc ttgaggtttc
    23701 cgaaaaatac actttgatcc tgcagttgta gcgcctcaca agtactgctg cccaggatag
    23761 gtaaacaagt gatgacaagg tcattgtgat aatcacaacc gggtaatccc actgatgttt
    23821 aaatgattcc gactgaagaa tgactatctt ttcgttcagg ggtttggtgt agtactcgaa
    23881 ggtgaacagt ataatgcaaa gcacaaaggg taacaggttc agcaggtaca ccggtctgaa
    23941 cctgaaatgc tggtcggtca gtgatttcac atacagccac agaatcggcc agtgaagcaa
    24001 cagaaacggg gtagagacaa aaaagaagag cgggtatggg aatccgtgcg tgcgattgta
    24061 tatttccagg taactcagaa aggtatttaa tgaataaacc aggaagaatg atgacagcag
    24121 gtagtctgag accgtcttct tccgtttcag gatgatcaga agaaagatta tcaatgcttc
    24181 aaatactcca aacagtagaa tgggttccat tgtatcttca ggtagtcaaa aaggtacacc
    24241 aaattacttt tttcctttaa gaaatgcatt gtcaatgatt aacaattgtt tgaaatttgc
    24301 caaacctgct gacttaatta cagattcatt ggcgggaaga ttgccggctc cggatctggc
    24361 gggctgttat ggggtggata catcagggag aagattgctc gcccttcctt cggtcgggac
    24421 tcgcggactt ccatggggcc aaccggaaga gaaaaatgac cttttcgctt cactcaaagg
    24481 ttctttttta ctgccctttc tcttacaaca gcaagctgtt gacgctacag gacaataaaa
    24541 aaaccaccac cggaaaaccg gcggtggtca tttttctctt gtcggggtga gaaga
//
LOCUS       DAOPXU010000035        24528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_2040_length_24528_cov_13.896825, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000035 DAOPXU010000000
VERSION     DAOPXU010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24528
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2040_length_24528_cov_13.896825"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PKK03_05240"
     CDS             <1..519
                     /locus_tag="PKK03_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNX83835.1"
                     /translation="SETVVFRSRVMGLLTVRKRGDLLELDFPVDHVTECALPGIIKES
                     LGAHPVEWYAGRSDYLLVYRSESEIREMKPDFRKLAQTESRGVIVTAPGDTVDFVSRF
                     FCPQIGIDEDPVTGSAHTTLTPFWSARLGKTTMAARQLSSRGGMLECALDGDRVRIAG
                     SALLYLKGELFI"
     gene            563..1030
                     /locus_tag="PKK03_05245"
     CDS             563..1030
                     /locus_tag="PKK03_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HNX83836.1"
                     /translation="MTKKDAIHPYGEKQEQFDRRYLRMAAVWSENSYCQRRKVGALIV
                     KDKMIISDGYNGTPAGFENVCEDENNVTKPYVLHAEANAITKVARSNNSSENATLYIT
                     DSPCMECAKLIIQSGIRRVVYSNQYRNPDGIELLKRADIELLYIDFGEKKNEI"
     gene            1020..2612
                     /locus_tag="PKK03_05250"
     CDS             1020..2612
                     /locus_tag="PKK03_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX83837.1"
                     /translation="MKYNKAYLPLIIGIAVAIGILIGFYMPHPQTGLYTNFPPSNDKL
                     SRVLDIIESDYVDTVNRNALIEEAIPVMLRKLDPHSVYIPATDLAQANEPLQGNFDGI
                     GVSFNMLTDTVLIISTIPGGPSEKAGIIPGDKIILVNDSLIAGKNIPDEDVVKMLKGP
                     RGTRVDLKIERKGAKELLPFTITRDKIPIYSVDVSYMMSDDVGYIKISNFALTTHDEF
                     KKGLNELKETGMSKLILDLRGNSGGVMDAAIQIADEFLPEGKLIVFTMGKSTPRQDMK
                     ATDQGEFETGELVVLIDEWSASASEILAGAVQDNDRGTIIGRRSFGKGLVQEPVMFRD
                     GSGMRLTIARYYTPTGRSIQKPYDQGVDKYYEDLGNRLMHGEFEEADSIRHDDSLRFT
                     TPGGKTVYGGGGIMPDVFIPLDTTGISDYFLAIRNSGLLYRYSLKFTEDNRETLRKYN
                     DLPALIGWLDSQNLLSKFITYAGQNGVPARKDQIEISKKVIEVQLKAYIARNMLDNKG
                     FYPVWQEIDKTLLDALSYLENN"
     gene            complement(2620..3420)
                     /locus_tag="PKK03_05255"
     CDS             complement(2620..3420)
                     /locus_tag="PKK03_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX83838.1"
                     /translation="MKRSIEFEGVRISYTVRGKGKAIILLHGYLEGGEVWDPLTERLE
                     EEYRIITPDLPGHGESGVKGEVHTMEFLASAMREVIRDAGENRILMVGHSLGGYVTLA
                     FVEMYPELLSGYVLFHSHPHADTPEAVARRNREIAVVRAGRKNIMYPGNISMMFAKEN
                     LRPMHAQLERSRKIASRNPGEGIIAMLNGMIVRPSRQYVLEKGAVPLLWILGRHDLYF
                     SPEKAQRDIGLPLNAGVVILENSGHLGFIEETEKSTFLLKDFASRITW"
     gene            3517..4161
                     /locus_tag="PKK03_05260"
     CDS             3517..4161
                     /locus_tag="PKK03_05260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HNX83839.1"
                     /translation="MNKILFSAVSKKFVMALAGLFLLLFLPVHLGINLMLLGSDPEPF
                     NNAAHFMATFPLVKVVEIVLILAILVHITYGLILQIQNWRSRPVRYAVRNKSETTAFS
                     KFMIWTGGSVLIFLVIHFFNFYFIKLGIVEGDPENFYSVAHQLFGIRGYVILYWICFL
                     LLGFHLYHSLQSAFQTLGWSNEFWKPVIQVISLIYAIIIPAGFAVIPLVIQFVK"
     gene            4181..6097
                     /locus_tag="PKK03_05265"
     CDS             4181..6097
                     /locus_tag="PKK03_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HNX83840.1"
                     /translation="MITLNAKIPEGPLGEKWTRYKAASKLVNPANKKKLDIIVIGTGL
                     SGAGAASSLGELGYNVKVFCYQDSPRRAHSVAAQGGINAAKNYKNDGDNVYRLFYDMI
                     KGGDFRAREANVYRIAEVSNQVIDHYTALGVPFAREYGGTLDTRSFGGVQVSRTFYAK
                     GQTGQQCLLGAYSSLNRQIKAGTVKMFPRRDMLELVVIDGKARGIITRNLITGEIERH
                     SAHAVVLATGGYGTVYYLSTLAVNSNASAAWKAYKKGAAFANPSFVQIHPTSIPQLNE
                     FQSKLTLMSESLRNDGRIWVPKQKGDTRPANDIPEEERDYYLERRYPAFGNLVPRDVA
                     SRAAKERCDAGYGVGKTGLAVNLDFRDAIKKQGKKSIENKYGNLFDMYRAITGMNPYE
                     EPMQIYPAGHYTMGGLWVDYELMTTIPGLFAIGESNFSDHGANRLGASSLMQAAGDGY
                     FILPVTISSFLSGEIRIPRIPTDGPEFDEAEKNVRERLEKLLSIKGKQSAASFHRRLG
                     KIMWDYCGMTRNEAGLKKAKDMVRELRSEFWSDLKVTGEGDDLNQQLEKAGRVADFLE
                     LAELMITDALNRKESCGAHFREESQTPDGEAMRIDSEYSYVAAWEYNGGDEPILNKEP
                     LKFEFVELKERSYK"
     gene            6119..>6780
                     /locus_tag="PKK03_05270"
     CDS             6119..>6780
                     /locus_tag="PKK03_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNX83841.1"
                     /translation="MKLRLKIWRQKNAEAKGAFASYDLDGVSPDMSFLEMLDLLNSRL
                     VSESKEPVAFDHDCREGICGSCGMYINGYPHGPVPGLTTCHLSMRYFRDGETITIEPW
                     RAKPFPVIRDLIVDRSAMDKIQVAGGFISVTAGAAPEANSNLVRKHSSDLAFDSAICI
                     GCGACVAACKNASAMLFVSARVSQYALLPQGRPEAKERVRAMVEEMDRQGFGNCSNTG
                     AC"
     assembly_gap    6781..6880
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6881..7686)
                     /locus_tag="PKK03_05275"
     CDS             complement(<6881..7686)
                     /locus_tag="PKK03_05275"
                     /inference="COORDINATES: protein motif:HMM:NF017046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HNX83842.1"
                     /translation="MFEKEVYIKRRQRLKSLVNEGIALFIGNVEAPMNYPDNTYHWRQ
                     DSDFLYFFGIDLPGLAGLIDFNSGDEIIFGNDFTVDDIVWMGPQPSVAELASRAGIMH
                     THPMDKLDEMVHDALKAGRTVHFLPPYRAETKMTLGALLKENPCQMRTRASEKLIKAV
                     VSLRSIKEDVEIVEIEKAADISCEMETTAMRMCKPGVTEQEIYGVLQGIAYSKGACPS
                     FPIILSINGQTLHNHVHVNVLKEGRMMVTDCGAESNMHYSADLTRTTPVGG"
     gene            7902..8576
                     /locus_tag="PKK03_05280"
     CDS             7902..8576
                     /locus_tag="PKK03_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83843.1"
                     /translation="MSKEIPPFRLAFSMKTLVKIQGFLPLFENSISHQRQMLVNQNEK
                     AKSNQETARKARLYLTHFLRVMNMAVTRGELPPETRTFYGVASDDSTLPSLATENELV
                     AWGKRIIDGEEYRIRKGYSPVTNPTIAVVKVRYEKFLETRNHCKTVNRRASECVNKTS
                     DMRQEADDLITRLWNEIEASFSSLTEEEKRTSAEKYGLVYVFRKHEMTRKDLSPTLFS
                     ADQENQ"
     gene            8584..9597
                     /gene="dusB"
                     /locus_tag="PKK03_05285"
     CDS             8584..9597
                     /gene="dusB"
                     /locus_tag="PKK03_05285"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNX83844.1"
                     /translation="MKLGNTILREQPLFLAPMEDITDPSFRYVCKMFGADFMYTEFIS
                     SDGLIRDGRSSVKKLELFDYERPIGIQLYGHLTESMVDAALRAEEANPDVIDLNFGCP
                     VRKIAARGAGAGMLQNIPLMTEMTSAIIRAVKIPVTVKTRLGWDENSRIIVDVAERLQ
                     DTGIAGLTIHGRTRAQLYRGEADWTLIGEVKKNPRMKIPVIGNGDINGPERAKEMFER
                     YGVDGIMIGRATFGRPWIFREIRHYLDTGEILPEPTVEEKADLALLHLDKSLEHKEGK
                     PAILEMRRHFSNYFKGLPGFKETRLKLLTSTDVDEIRSIIGEIREKWGEGRYGRSTGI
                     YGI"
     gene            complement(9574..10578)
                     /locus_tag="PKK03_05290"
     CDS             complement(9574..10578)
                     /locus_tag="PKK03_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNX83845.1"
                     /translation="MEVNEIIDAPENRSESPFITVAFTGKNSFWRYCIGVVGPFLAAN
                     FIGGLPLAAVIIAGALDGSAIPQKGTMPDFEAMGINLNVGFVLNVIPFLIGLLAFILL
                     VKPLHNRTFGTVVNGGRKVRWGRMMFAALAWIAVSALYMLYALKSDPGNFRINNTSNS
                     LIVLAVLALLLIPFQAAFEEVLFRGYLMQGLAVLARNRWIPVLSTSILFGLMHGLNPE
                     VMQYGFLTMMPQYIFFGIVFAVLTMFDDGIELAIGAHAANNIFLSVLVTHEDMALQTP
                     ALYEQIQIYPWKEFGGLVLQSLIFLAIMALVFKWKEIRRLYARINAPAAEVSDAVDAG
                     "
     gene            complement(10590..12167)
                     /gene="nadB"
                     /locus_tag="PKK03_05295"
     CDS             complement(10590..12167)
                     /gene="nadB"
                     /locus_tag="PKK03_05295"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNX83846.1"
                     /translation="MKIRCDYLVIGSGIAGLSFALKAAAHGKVCVITKAKMDDCSTNY
                     AQGGIAAVMYEPDNFEKHVKDTMVAGAGYCNEEVVRMVVSEAPERIRELIEMGVDFDK
                     KPDGTLDMAMEGGHTEHRILHHKDSTGAAIEKVLIEKAREHRNIRIYEDHFAIDLLTQ
                     HNLGEYVTREWKNIECYGAYVADLETSKVTTFLAGVTVLATGGCGNIYLTTTNPPVAT
                     GDGVAMVHRANGALENMEFIQFHPTSLYNPRERPSFLITEALRGFGAELRNRAGEKFM
                     PKYHVRGSLAPRDVVARAIDNEMKLKGDDFEWLDCTHLDADELRSHFPHIYQKCLSEG
                     VDITRDMIPVVPAAHYSCGGVRVDMNGRSSIDRLYAIGEVSSTGLHGANRLASNSLIE
                     AAVYAHRAAGDASARIGSLTHEEKIPEWNYHGTTHLEEMILVTQNYREMQMIMSNYVG
                     IVRSDLRLARAKTRLEIIYQETEDLYRRSTISRELIELRNLIAAGYLVIKMAQNRQES
                     LGLHYSIDYPARPGSEF"
     gene            12292..13842
                     /locus_tag="PKK03_05300"
     CDS             12292..13842
                     /locus_tag="PKK03_05300"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNX83847.1"
                     /translation="MAQEDVFRKLVAHCKEYGYVFPSSEIYDGLGAVYDYGQYGVELK
                     NNIKKYWWDSMVLLNENIVGLDSAIFMHPTIWKASGHVDAFNDPLIDNRDSKKRYRAD
                     VLIEEHLDKFNEKIEKEVEKARGRFGEKFDEALYRSTNPRILETREKYDALHKRFVDA
                     MAGPDLAELRQIIVDNGIVCPVSGTCNWTEVRQFNLMFATEIGSTSDGAMKIYLRPET
                     AQGIFVNFLNVQKTGRMKLPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVKPG
                     TELEWFSHWKEARMRWHMALGFGENNYRFHDHDKLAHYANAAADIEYRFPFGFKEVEG
                     IHSRTDFDLRQHQEYSGKKMQYFDPEMNESYTPYVIETSIGVDRMFLQVVAASYFEED
                     LTKEDGTPDSRVVLRLPAVLAPVKLAVMPLVRKDGLPDISRKIISDLKFDFNCQYDEK
                     DSIGKRYRRQDAIGTPYCITIDYQTVQDETVTIRYRDSMEQERVPVSQLRAIIADKVS
                     MQEQLKKL"
     gene            13844..15127
                     /locus_tag="PKK03_05305"
     CDS             13844..15127
                     /locus_tag="PKK03_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX83848.1"
                     /translation="MRAGPAKVLIITYYWPPSGGAGVQRWLKFTKYLPSSGWFPVVLT
                     VDPEYAAYPFRDESLYGEIPVDVEVHRTKALNFFALYNRDQSKIPHAGFASGKDKGIK
                     SAVSRFIRGNFFIPDPRRGWNRFAFRKACELIRSQNISHIITTSPPHSTQLIGLRMKR
                     RFPGLKWIADLRDPWTDIYYYDMFRPSLPARMLDRNLEKRVLSRADRIITVGQALASV
                     FAAKAETVADKTEILPNGFDEEDFQGVASSVPERFTITYVGTLSPAYPVRGFIEAVKD
                     IRIDGKQVALQFVGTVPEEVRVLFPAGDEGGAPRFISYCEHPEAIRHMMSSSLLLLII
                     PDHRSNRSILTGKIFEYLATEKPILYLGPEDGDAARLLALCGYQGIFGYDNVEGIREF
                     ILRVAEGKTVKRSGHHLEYSRRALAVKLGNILDRL"
     gene            complement(15196..16677)
                     /locus_tag="PKK03_05310"
     CDS             complement(15196..16677)
                     /locus_tag="PKK03_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83849.1"
                     /translation="MGIVQRQTIRGTAWSYLGALLGFVNIILLSPKIFTTGEIGVVQL
                     LLSFATMLAQFSSLGFTNVINRLFPYFRGIRGRHHGFLGLAVVITLAGFIAALVFLKY
                     YAPHFEQANLERSPLISRYSIYLPALLLVTVLFNLLDNYNKVLYDAVLGTFLREFLFR
                     VLNLGLIMLFWAGIIDFDSYVFGYVVSQGVPLIIIFISLLIRGEISLRLEPGFITPHL
                     KREIIILSLFGILTGISTVTLTTLDKLFINTYLGEAEVGIYSIASYFAVLITLPGRSV
                     SKISIPFLAESWKKNDLEKIEDMYVRSSVSQYAVGLLIFIGLLVNIDNIFRLLPDIYS
                     KSAAVIVLISFGNLVSGSAGVNGVVLSTSSLYRYQTWLMFILIGLFVGTSLIFIPLFG
                     ITGAALASMISNIIYSMLGVLLTGKRFGLWPYNILHLKMTLVGLAALVAGYFIPQMPL
                     ISDIIARSLLVTVIFTAGVWFWKLSDDLNGVAESLMDRFRRKR"
     gene            16794..19250
                     /locus_tag="PKK03_05315"
     CDS             16794..19250
                     /locus_tag="PKK03_05315"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HNX83850.1"
                     /translation="MKPSSFKSALPHIIAVLLFTVVSFAYFFPVLEGKKINAHDTKVY
                     EGSSKEISDYREKHGGEPLWTNSMFSGMPAYMISVKYPGNLFKHLDNFLKVFKTPVAA
                     LFLSMAGFYIMLLLFGVNPWLAMAGAIAYGFSSFLFASLSAGHNTKAYAMAYMAPVIG
                     SVVYSFRRNTMTGASLFALFLALQIMANHVQITYYTGIIVLVFGIYELIHAVKSRMIP
                     SFLKSLGVLVVAALIAFSVNFASLYSTWEYSKESTRGKSELTTAAGTNTKGLDPEYIT
                     QWSYGIGESMTFLIPNFRGGATAPFDSNSETVKALRKNNMGQAQGQLYRYWGKQPNTS
                     GPVYFGAVVLFLFVLGLMLIKGRDKWWILTAALLSLLLSWGKNLMPLTTLFIDFFPGY
                     DKFRAVSMTLVMAGVLVPLLGVLALREISEGTVSREKAARSVLIAAMVTGGLALIFFL
                     IPGLAGSFLRPDEIALPDSAAWIKDAMATDRKMMIRTDALRSAVFIAGAAAVIWFYLK
                     NKLKPAYALMGLTVLFLIDQVPVDARFLGSSSFETKRASANSFAPSEADKIILGDEGE
                     FRVLNLAVSVFNDASTSYHHHSIGGYSGAKMKRYQELIETSLSDDINSLITNLRTATT
                     MEEAEGVLGSLGTLNMLNTKYIILDPGSAPLVNRHAAGNAWLVNRVTLAENADAELQA
                     VKTINPREEAVVDRRFAELIRVAETAGAPGDTIFLTSYEPNLLTYQAELSSDRVAIFS
                     EIFYKYGWKASVDDKPADHFRANYVLRGMTLPAGSHTVTFRFDPESYRTGNRVSLAGS
                     VLLLAFLLLAALPLLKKRSR"
     gene            19250..20146
                     /locus_tag="PKK03_05320"
     CDS             19250..20146
                     /locus_tag="PKK03_05320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX83851.1"
                     /translation="MAERLICPACGSADIAGLFHAKDHLVSSREFLIMRCNACGMGWT
                     ADPPPEEEAGNFYVSEEYISHTDRKQSISDYIYHLARSFMLGRKQRLVASAVGKETGS
                     LVDIGSGTGYFAAYMKKRGWQVTGIEISDNARKYSVARFGIRAVTPAEIKGMPPDSAD
                     CITLWHVLEHLYEPDLWMDEVNRILRDDGKCIIALPNFASSDSARFGNRWAALDVPRH
                     LWHFTPAAFVNFADRHGLACEKISALPLDLFYISYLSYRNESKSMPLIRGAVTGLFIA
                     LRNIFRKKKASSLVFVISKKKS"
     gene            complement(20143..21147)
                     /locus_tag="PKK03_05325"
     CDS             complement(20143..21147)
                     /locus_tag="PKK03_05325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNX83852.1"
                     /translation="MCRSEGVHKVKRGLIILAAIMITSVLDAQQLPIYSQYLFNKYLI
                     NPAVAGSDGYTSLNLTTRQQWAGYQGAPQTYSLSFNGRLLKQKYLIRQNVFDKKIFKP
                     HTEGRVGIGGTVFSDINGLVRRTGFSTSYAYHIWLQGGTQLSLGLAFTGYHYKIDQRQ
                     IQFEDPDEPWLNTDFRKGIFVPDFNFGAYLLNRKYAIGFSAQELMEGFIKAGSQAYRD
                     LKIQRTYYLFGNYDFEPDNKTLIQPSLLIKMSEQVRPQADIGVTYSYDNKLWVGATFR
                     TGSAIIANVGVRRDRLFFGYSFDFTLNSIQMSTYGSHEFIVAARFGDTPRKYRWLDRY
                     "
     gene            complement(21151..>24528)
                     /locus_tag="PKK03_05330"
     CDS             complement(21151..>24528)
                     /locus_tag="PKK03_05330"
                     /inference="COORDINATES: protein motif:HMM:NF040284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNX83853.1"
                     /translation="QNQIINISDLLTNNSDSYQTAIYRFRPYIQGKTGDPTCHDGVEV
                     IIAVHVEPTARVALTLSDNELCNGESVNIQIATPTTAYNGLEFNVEAINSYPEITGFT
                     DRTGLIVTDIITEALSNSGDTARLVTYVVAPATLDVSGNQKCYGIRDTVRVWVNPTPR
                     ATPENYAPHICYGGTTDVVLLSPTVMSSGINEFNYTITSTASTAVVAGNRTALNSQAP
                     GTHLQFPYTNESDTMQSVFFRITPKVTGPGCPYGPPVISEIKVHPHPLQSLEILDSIT
                     CDGGQDGALQVIYARGFDSLWVAWTGPDYWEEAAYNMFIAEECSQGYYTATVTDSLGC
                     SSSDALNFLAPSIEVSLYFGQFISCPGAEDARMVVALTEGHAPPYYYSLVWNSADTVY
                     QGWMPPEGTFFSLDNLKPGEYLLTVVDANGCREELPRTLYDAPVTFAGFEKSLYADDN
                     ISCESYSDGTIRVSEIYSYYMEGADTVFVSSRAPYTYLWSASNGGIISGSPTDSLLVN
                     IPAGTYTLTVTDSQGCIFNFTETMTEPDGIDLIDEEVSLSNDGNYEISCYGGSDGFIN
                     LQFDGGTGAYGYAWTGPDGFTSNTQSLTGIGAGTYNLTVTDGNMCHREYTYILDEPDS
                     LSIAVSTSHTPDNAFNIGCNGGDGTIDITVSGGSGAGTYSYQWTKSADQSYASNLEDL
                     TVKAGTYLLQVTDVNGCVNTRTVEITEPDSLSLSLAVSDITCLNAPAYNDGSIDLTVS
                     GGRPPYSYSWTGPAGFTSSLEDISALTEGTYTITVTDSYGCSIASDTFLSLPEPISLE
                     PRISDYNGFSISCLGKSDGWLRIIPQTGTAPYSYSWSGPAGFTATTDSIYSLREGTYS
                     VIVTDAHLCSVTQDITLVSPGQLSMTLSIGLSNGGSFNINCNGASSGRVNVTAVNAAG
                     TPEYIWSDGLSGAARDNLTAGYHEVILTDANGCIADTSLTLTEPDSLRISFALISPYC
                     PESNDGSIFAGVTGGEGAYTFTWSDGQTTQEAVGLVQGLYTVEARDFNGCVLSDSLLL
                     KPTNDICVGIPNAFSPDGDGINEFWNITRISFYNEAEVIILNRWGEIVWKSEKGYPDP
                     WDGRASNGKVLPMDSYHYAIDLHNGEKPIVGHVTIIR"
BASE COUNT         6228 a         6245 c         6242 g         5713 t
ORIGIN      
        1 agcgaaacgg tagtattccg ctccagggtg atgggacttc tcactgtcag gaaaagaggc
       61 gatctcctcg agcttgattt ccctgttgac catgtgacag agtgcgcgct gcccggaata
      121 atcaaagaaa gcctgggcgc tcatccggtt gagtggtacg ccggaagaag cgattatttg
      181 ctcgtttaca ggagcgaatc agaaatacgt gagatgaagc cagacttcag gaaactggcg
      241 cagacggaaa gcagaggcgt cattgtaacc gctccgggtg acaccgttga tttcgtctca
      301 aggttcttct gtccgcagat tggaatagat gaagatcccg taaccggatc ggctcacacc
      361 acactgaccc ccttctggtc agcccgcctg ggaaagacca ccatggcagc aaggcagctg
      421 tcatccaggg gcggcatgct tgaatgcgcc ctcgacggag acagggtacg catagcaggc
      481 tcagcactac tgtatcttaa aggtgagctt ttcatctgat ttagcttaaa aaccattaag
      541 tttgccggcg aaacagaaga ggatgacaaa aaaggatgcc attcacccct acggggaaaa
      601 acaggagcag tttgacaggc gttacctgcg gatggccgca gtgtggtcag aaaactccta
      661 ctgccagagg cgaaaggtgg gagcgctgat agtcaaggac aagatgatca tctccgacgg
      721 atataacggt actcctgccg gatttgaaaa tgtctgcgag gatgagaata atgttaccaa
      781 gccctacgtt ttacatgctg aagccaatgc catcaccaag gtagcacggt caaacaactc
      841 aagcgaaaac gccacactgt acatcaccga ctcaccctgc atggagtgtg caaaacttat
      901 catacagtca ggtatcaggc gggtagtcta cagcaaccag tatcgcaatc ctgacggcat
      961 cgagcttctg aaacgggctg acatcgagct tctttatatt gatttcggag aaaagaaaaa
     1021 tgaaatataa caaggcgtac cttccgctga taatcggaat cgcagttgcc attggcatac
     1081 tgataggttt ttacatgccc catccccaga ccgggctcta tacaaacttc ccgccgtcaa
     1141 atgacaaact gagccgggta cttgacatca ttgagtctga ttatgttgat accgtcaaca
     1201 gaaacgctct gatagaggag gccatacctg taatgctccg caagcttgac cctcactcag
     1261 tctacatccc ggccaccgac ctcgcacagg cgaacgaacc actgcaggga aactttgacg
     1321 ggataggcgt ttcattcaac atgctgactg acaccgtgct tataatcagc accatacccg
     1381 gagggccgtc ggaaaaggca ggcatcatac cgggtgacaa gataatcctg gtcaatgact
     1441 cactcatcgc cggcaaaaac attcccgatg aggatgtggt caagatgctg aagggtccac
     1501 gcggcaccag ggtcgacctg aagattgagc gcaagggcgc aaaggagctc cttcctttca
     1561 ccatcacccg ggacaagatt ccaatctata gcgtagatgt atcatacatg atgtcagatg
     1621 atgtaggata tatcaagatc tccaacttcg ctctcaccac ccatgatgaa ttcaaaaagg
     1681 gtctcaatga gcttaaggaa acaggaatga gtaaactgat cctcgacctc aggggaaact
     1741 ccggaggtgt gatggatgca gcaatacaga tagctgacga gttccttccg gaaggaaagc
     1801 ttatagtctt caccatgggc aaatcgacac cccgccagga tatgaaggcc accgaccagg
     1861 gcgaattcga gacaggagag ctcgtagtgc tgatagatga gtggagcgcc tcagccagtg
     1921 agatcctggc aggtgcggta caggacaacg acaggggcac aatcattggc agacgctcat
     1981 tcggcaaagg cctcgtacag gaacctgtca tgttccgcga cggatccggt atgaggctga
     2041 ccatagcccg ctactacacc ccgaccggcc gttctattca gaaaccctat gaccaggggg
     2101 tcgacaagta ttacgaagac cttggcaacc ggctcatgca cggtgagttt gaagaggctg
     2161 actcaataag acatgatgat tcactgcgct tcaccacccc gggaggcaag acagtctacg
     2221 gtggtggcgg aatcatgccc gatgtcttta tccccctgga caccacagga atttcagact
     2281 atttcctcgc aatcaggaac tccggcctgc tatacaggta ttccctgaaa tttactgagg
     2341 ataaccgtga aaccctcaga aaatacaatg acctgccggc actgatagga tggctcgaca
     2401 gtcagaatct cctttcgaaa ttcattacct atgcgggaca gaacggcgta ccggccagaa
     2461 aggatcagat cgaaatctca aaaaaggtaa tagaagtcca gctgaaagca tatatcgcac
     2521 gcaacatgct tgataacaag ggcttctatc ctgtctggca ggagattgac aaaactctct
     2581 tggatgccct cagttacctg gaaaacaact gaaatctcat caccaggtta tccggctggc
     2641 aaaatccttc agcaggaagg ttgatttttc agtttcctcg atgaagccga ggtggcctga
     2701 attctccagg atgacgaccc cggcgttgag aggcaggccg atgtcacgct gagccttctc
     2761 aggagagaag taaagatcat gcctcccgag aatccataac agcggaactg cgcccttttc
     2821 aaggacatac tggcgtgaag gcctgactat cattccattc agcatggcaa taatcccttc
     2881 gcccggattc cgcgaagcga tcttcctgga cctttcaagc tgtgcatgca tgggccttag
     2941 attctctttc gcgaacatca tgctgatatt ccccggatac atgatattct tcctcccggc
     3001 cctgacgacg gcaatctccc tgttacggcg tgcaactgct tcgggggtgt cagcgtgggg
     3061 atgagagtga aaaagcacat atcctgacag cagctccggg tacatctcca caaatgcaag
     3121 tgttacatac ccgcccaggg agtgaccgac catcagtatc ctgttctccc cggcatccct
     3181 gatcacttcg cgcatagctg aagcaagaaa ctccatggtg tgtacttcac ccttcacccc
     3241 ggattcacca tgacccggca ggtcaggggt gatgatccgg tattcttctt ccagcctctc
     3301 agtcagtgga tcccatacct ctccgccttc aagatagcca tgcagtaaaa taatggcctt
     3361 ccctttgccg cgcactgtgt aagagatcct taccccttcg aattctatac tccttttcat
     3421 cagaacttgt ttaatgaaaa aaacttctaa atttacgggt aagtaagaaa tttctcagat
     3481 ttttcacaaa acctgttata ataaagtaaa aagccgatga acaagatcct cttctcggct
     3541 gtctcgaaga aattcgtgat ggcccttgcc ggtttgttcc tgctgctgtt cctgcctgtc
     3601 catctcggca taaacctgat gctcctggga agtgatccgg agccctttaa caatgctgct
     3661 catttcatgg ccaccttccc gcttgtaaag gtggtggaaa tagtgctcat cctggcaatc
     3721 ctggttcaca tcacttacgg gctcatcctg cagattcaga actggcgctc taggccggta
     3781 agatatgcgg tgagaaacaa atccgaaact acggccttct cgaaattcat gatatggaca
     3841 ggaggatcgg tgctgatctt cctcgtgatc catttcttca atttctattt cattaagctg
     3901 ggaattgtcg aaggagatcc ggaaaacttc tattcggttg cgcatcagct gtttggcatc
     3961 cggggttatg tgatccttta ctggatctgc ttcctgctcc tcggtttcca tctctatcac
     4021 tccctgcagt cggcatttca gacacttggc tggtcaaatg agttctggaa acccgtaata
     4081 caggtgatct cactaatcta tgccatcatc atcccggcag gatttgcggt tataccgctt
     4141 gtcatccagt ttgtcaaata acccgaagaa tccaacagca atgataactc tcaacgcaaa
     4201 gatacccgag ggtccgctcg gggagaagtg gacaaggtac aaggccgcct caaagcttgt
     4261 taacccggcc aataaaaaga agcttgacat aattgtgatc ggtaccgggc tctccggcgc
     4321 cggcgctgcc tcctcactgg gcgaactggg atacaatgtc aaggtcttct gctaccagga
     4381 ctcgccccgc agggcacact ccgttgccgc acagggaggc atcaatgccg ccaagaacta
     4441 taagaatgac ggtgacaatg tctaccggct cttttatgac atgatcaagg gaggcgattt
     4501 cagggcacgc gaggccaacg tttaccggat tgcagaggtg agcaaccagg tcattgacca
     4561 ttataccgca ctgggagtac cctttgcccg cgagtatggc ggcaccctcg atacccgctc
     4621 attcggcggt gtacaggtgt cacgtacatt ctacgccaag ggacagaccg ggcagcaatg
     4681 cctgctcggg gcatactcat cgcttaacag gcagattaaa gccggaacgg taaaaatgtt
     4741 tccaaggcgt gacatgcttg aactggttgt gattgacggc aaggcacgtg gcatcatcac
     4801 ccgcaacctg atcacaggtg agatagagag gcattcggcc catgcggtgg ttcttgccac
     4861 cggaggctac ggcaccgtat attacctgtc gaccctggca gtcaacagca acgcttcggc
     4921 agcctggaag gcatataaaa agggggcagc cttcgccaat ccgagcttcg ttcagattca
     4981 cccaaccagc ataccgcagc tgaacgagtt ccagagcaaa ctgacactga tgtcggaatc
     5041 actccgcaat gacggacgta tctgggtgcc caagcagaag ggagacacca ggccggcaaa
     5101 tgatataccc gaagaggagc gtgactatta cctcgagcgc cgctacccgg cattcggcaa
     5161 tctcgttcca cgcgacgtgg cctcaagagc tgccaaggag agatgcgatg caggttacgg
     5221 cgtgggaaag acagggctgg ccgtaaacct cgacttccgc gatgccatta agaagcaggg
     5281 caaaaaatcc atcgagaaca aatacggtaa cctgtttgac atgtacaggg caatcaccgg
     5341 gatgaatccc tatgaggaac ctatgcagat ttatcccgct ggtcattata caatgggcgg
     5401 gctatgggta gactatgagc tgatgaccac catcccgggc ctgttcgcca taggcgagtc
     5461 aaacttctcc gaccatggag ccaacaggct gggcgccagt tcactgatgc aggcagcagg
     5521 agacggatat ttcatcctgc cggtcacaat ctccagcttc ctttccggag agatcagaat
     5581 cccccggata cccaccgacg gacctgagtt tgatgaggct gaaaaaaacg tcagggagcg
     5641 actggaaaag cttctctcaa taaagggcaa acagtctgcg gcaagcttcc acaggcgtct
     5701 cggcaagatc atgtgggact attgcggaat gacccggaac gaggcaggac tgaaaaaggc
     5761 aaaggatatg gtcagggaac tgcgcagcga attctggagt gacctgaaag tcaccggcga
     5821 gggggatgac cttaaccagc agctcgaaaa ggcaggccgc gtggccgatt tcctcgagct
     5881 ggctgaactg atgatcactg acgcacttaa caggaaagag tcgtgcggag cccatttccg
     5941 cgaggagagc cagacgcctg acggcgaagc catgaggata gactctgaat attcctacgt
     6001 ggcagcatgg gaatacaacg gcggggatga acccattctc aacaaggaac cgctgaagtt
     6061 cgagtttgtt gaactgaagg agagaagcta taaatagtaa cttttaacca atcctgatat
     6121 gaaactcaga cttaagatat ggaggcagaa gaatgcggaa gccaaaggcg cttttgcatc
     6181 atatgacctt gacggcgtct cccctgacat gtcatttctc gagatgcttg acctgctgaa
     6241 cagcaggctg gtaagtgaga gtaaagagcc ggtggctttt gaccatgact gccgcgaggg
     6301 gatctgtggc agctgcggaa tgtacattaa cggctatccg cacggtcccg taccgggact
     6361 tacaacctgt cacctttcga tgaggtactt cagggacggc gagaccatta ccattgaacc
     6421 atggcgggca aaaccgttcc cggtgatcag ggacctgatt gttgaccgca gtgctatgga
     6481 caagatccag gtagcaggcg gattcatttc cgtgacggcc ggtgcggcgc cggaagcaaa
     6541 cagcaatctt gtccgcaagc acagcagcga cctggccttt gactccgcaa tatgtatcgg
     6601 atgcggtgcc tgcgtggcgg catgcaagaa cgcctccgcc atgctgtttg tatcagccag
     6661 ggtgtcacag tatgcactgc tgccccaggg ccggcccgag gcaaaagaaa gggtacgggc
     6721 aatggttgag gagatggaca ggcagggatt cggcaattgc tcgaacaccg gcgcctgcga
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cctcccaccg gagtggtacg
     6901 ggtcaggtca gccgaatagt gcatgtttga ctcggctccg cagtcggtta ccatcatcct
     6961 cccttcctta agcacattta catgcacatg attgtgaagg gtctgcccgt tgatgctcag
     7021 tattatcggg aaggaggggc aggctccctt tgagtaggct atgccctgca gcacaccata
     7081 tatctcctgt tctgtaactc cgggcttgca catgcgcata gccgttgtct ccatctcgca
     7141 tgatatgtca gctgcctttt caatctcaac tatctcaaca tcttccttga ttgacctcaa
     7201 tgaaaccaca gccttgatga gcttctccga agccctcgtg cgcatctgac agggattttc
     7261 cttcaggaga gcccccaggg tcatttttgt ttcagcacgg tatggcggga gaaaatggac
     7321 tgtccttcct gccttcaggg catcatggac catttcatcc agtttatcca tcggatgcgt
     7381 atgcataatg ccggcccggg aagccagctc ggcaaccgat ggctgaggtc ccatccatac
     7441 tatgtcgtca acagtaaaat cgttgccgaa aataatctca tcccctgaat tgaaatcgat
     7501 cagtcctgcc agtcccggca gatcaatacc gaaaaaatag agaaaatcac tgtcctgtct
     7561 ccagtgatat gtattgtcag ggtagttcat cggtgcttca acattgccga tgaaaagggc
     7621 gataccttca ttcaccaggg acttcaacct ttggcggcgc ttaatgtaaa cttctttttc
     7681 gaacatatct gattctgttt tatgtaccag tacgctaatg taacaagagc aacggatttt
     7741 ttaccttatt aaagtcattt ttcgttgcga agggtattgt cagattcgtg taagaacgta
     7801 tattcgcata ttaacttcac taaaatcacc cgtttatgcc ctatcgccgc ctgcctaata
     7861 cagattctgc gcgtctgaga gcctttaaga aagccattga aatgagcaag gagattccac
     7921 ccttcagact cgctttctcc atgaagaccc ttgttaagat ccagggattc ctgccattgt
     7981 ttgaaaactc catcagccat cagaggcaga tgcttgtcaa ccagaatgag aaggctaagt
     8041 caaaccagga gacggcacgc aaggcaaggc tttaccttac tcatttcctg agggtcatga
     8101 atatggcagt gacaaggggt gagcttccgc ctgaaacaag gactttctac ggtgttgctt
     8161 ccgatgacag tacccttcct tccctggcaa ccgaaaatga actggtagca tggggtaagc
     8221 ggatcattga cggggaggaa taccggatca gaaagggata ttcacctgta accaatccta
     8281 caatagcagt tgtcaaggta cgttacgaaa agtttctcga gacgcggaac cattgcaaga
     8341 ctgttaaccg gagggcatct gaatgcgtga acaagacctc ggacatgcgt caggaggctg
     8401 atgatctgat aacacgcctc tggaacgaga tcgaagccag cttctcgtca cttaccgaag
     8461 aggagaaaag aaccagcgct gagaaatacg gtctcgtcta tgtattccgg aaacatgaga
     8521 tgaccaggaa ggatctctct cccaccctat tcagcgccga ccaggaaaac cagtaatcac
     8581 tcattgaaac tcggaaatac catactcagg gagcagccgc tgtttctcgc tcccatggag
     8641 gatatcacag acccctcatt caggtatgtc tgcaagatgt tcggtgccga tttcatgtat
     8701 accgagttta tctcatcgga cggactgatc agggacggcc gcagcagcgt caaaaagctt
     8761 gaactcttcg attatgagcg gccaataggt atccagcttt acggccattt aactgaatca
     8821 atggttgatg ccgcccttcg ggccgaagag gctaatcctg atgtcattga cctcaatttc
     8881 ggatgcccgg taaggaagat tgcagcacgc ggggccggcg caggcatgct tcagaatatc
     8941 ccgctgatga cagagatgac ctcagccatt ataagggcag tgaaaatccc ggtaacagta
     9001 aagacaaggc tcggctggga tgaaaacagc aggatcatag tcgacgttgc cgagcgtctg
     9061 caggatacag gtattgcagg actgaccatt cacggccgca cccgtgcgca gctgtaccgg
     9121 ggcgaggcag actggaccct cataggcgag gtaaagaaaa atccgcggat gaaaattcct
     9181 gtcattggca atggcgatat taacgggccc gaacgggcga aggagatgtt cgaaagatac
     9241 ggggttgacg gaatcatgat cggacgcgca acattcggaa gaccatggat attcagggag
     9301 atacgacatt accttgatac cggtgagata cttcccgaac ccaccgtgga ggaaaaggcc
     9361 gacctggctc tgctccacct tgacaaatct cttgaacaca aggaaggaaa acctgccata
     9421 cttgagatga gacgccattt ctcgaattac ttcaagggcc ttcccgggtt caaggagaca
     9481 aggctgaaac tcctgacatc gaccgacgtg gatgagatcc gctcgattat cggcgaaatc
     9541 agggaaaaat ggggcgaggg cagatacgga cggtcaaccg gcatctacgg catctgacac
     9601 ttcagccgcc ggagcattga tccgtgcata gagcctgcgg atttccttcc atttgaacac
     9661 aagtgccatt attgccaaaa aaatcagcga ctggagcacc aacccgccaa actccttcca
     9721 tggatagatc tgaatctgtt catacagtgc cggggtctga agggccatgt cttcatgggt
     9781 gacaagcacc gaaaggaata tattgtttgc agcatgagcg cctattgcca gttcaatgcc
     9841 gtcgtcaaac attgtaagaa ccgcgaagac aatcccgaaa aatatatact ggggcatcat
     9901 tgtgaggaag ccgtattgca taacctccgg gtttaatccg tgcatcagcc cgaagagtat
     9961 tgatgtggaa agtaccggaa tccacctgtt gcgtgccagg acggcaagac cctgcatcag
    10021 gtaacccctg aaaaggacct cctcgaaagc agcctggaat gggatcagta acaaagccag
    10081 gacagcaaga acgatcagtg agtttgatgt gttgttgatc cggaagtttc ccgggtcact
    10141 tttcagtgca tagagcatgt acagtgctga tacggcaatc catgcgaggg cggcaaacat
    10201 catccggccc cacctgacct tccggcctcc gttgacaacg gtcccgaaag ttctgttatg
    10261 cagtggcttg accagaagaa tgaatgccag caatccaatc aggaaaggaa tcacgttcag
    10321 tacgaatccg acattgaggt taattcccat ggcttcaaaa tcaggcatgg ttcctttctg
    10381 tggtattgca gagccgtcga gggcaccggc tatgattact gcggccagcg gcagtccgcc
    10441 tatgaagttg gcagcaagga acgggcctac caccccgatg caatacctcc agaatgagtt
    10501 cttcccggtg aaagcaacgg tgataaaggg tgactcactc ctgttctcag gagcgtcgat
    10561 gatttcattg acttccatat agctttttat taaaattcag atccgggtct tgccgggtaa
    10621 tctatcgagt agtgtagtcc caggctctcc tgcctgttct gggccatctt tattacaagg
    10681 tatcctgcag cgatcaggtt tcgaagctct atcagctccc gtgagatggt tgacctgcgg
    10741 tacaggtcct ccgtttcctg gtagataatc tctagccgtg ttttagccct ggcaagcctg
    10801 agatcagacc tcacgattcc gacgtagttg ctcattatca tctgcatctc ccggtagttt
    10861 tgggtaacga ggatcatctc ctcgaggtgg gtggtgccat gatagttcca ttcagggatc
    10921 ttttcttcat gagtaagtga gccgatgcgt gccgatgcat ccccggccgc cctgtgggca
    10981 tatacggctg cttcgataag ggagttggat gccagtctgt ttgcaccatg aagcccagtg
    11041 gatgaaacct ctcctattgc ataaagcctg tctatggagc tccttccgtt catgtcaacc
    11101 cttacaccac cgcatgaata gtgggctgcg ggtacaaccg ggatcatatc ccttgttata
    11161 tcaacgccct cagagagaca tttctgataa atatgcggga aatgagacct gagctcgtca
    11221 gcgtcaagat gggtgcagtc gagccattcg aagtcatcac ccttcagttt catctcattg
    11281 tcgatggccc tggccacaac gtcgcgcgga gcgagagatc cccttacatg gtatttcggc
    11341 atgaacttct ccccggccct gtttcgcagc tcggccccga aacccctcag agcctcggtg
    11401 atcaggaatg aaggcctctc cctcggatta tacagtgatg tgggatgaaa ctggatgaat
    11461 tccatgttct ccaacgctcc gttggccctg tgtaccatcg ccaccccgtc gccggtggca
    11521 acagggggat tggtagtggt aaggtatatg ttgccgcaac caccggtggc cagcaccgta
    11581 accccggcaa ggaatgtcgt taccttgctg gtttccaggt cagccacata ggcgccgtaa
    11641 cactcgatat tcttccactc cctggtaaca tactcgccaa ggttatgctg ggtgagcagg
    11701 tcaatggcaa aatgatcttc atagatccgg atgtttctgt gctcacgtgc cttttcaatc
    11761 agcaccttct caatggcggc gcctgtagaa tccttatgat gaagtatcct gtgctcggta
    11821 tgacctcctt ccattgccat gtcaagagtg ccgtcaggct tcttgtcaaa atcgactccc
    11881 atctctatca gctcccggat ccgctccggg gcttcgctga caaccattct caccacctcc
    11941 tcattgcagt atccggcacc tgcaaccatc gtgtccttaa catgtttctc gaagttatca
    12001 ggctcgtaca tcacagctgc tattccaccc tgggcatagt ttgtgctgca gtcatccatc
    12061 ttcgcctttg tgatcacgca cacctttccg tgggctgcag ccttgagcgc aaaagagagc
    12121 ccggctatac ccgagcctat caccagataa tcacacctaa ttttcataca ggtttcttaa
    12181 gaaacgaaaa gtaaagatac aaattgattt cagacctgaa gccggattgt aaaaccgcgg
    12241 attttaatat acttttgccc ggttgttatc acgaagaacc tcagaatcaa tatggctcag
    12301 gaagatgttt tcagaaaact cgttgcacac tgcaaagagt acggatatgt attcccctca
    12361 agtgaaattt atgatggcct gggcgctgtc tatgactatg gccagtacgg cgttgaactg
    12421 aaaaacaata tcaagaaata ctggtgggac agcatggttc ttctcaacga aaatattgtg
    12481 gggcttgact ccgcgatatt catgcacccg accatctgga aagcttcagg ccatgttgac
    12541 gcattcaatg atccgctgat tgacaaccgt gacagcaaga agcgttaccg cgctgacgtg
    12601 ctcatcgaag agcaccttga caagttcaac gagaagatcg aaaaggaggt cgaaaaagcc
    12661 aggggcagat tcggggaaaa atttgacgag gcgctttaca ggtcaaccaa cccccgcata
    12721 ctcgagaccc gggaaaagta tgatgccctg cataaaagat ttgttgatgc aatggcaggc
    12781 ccggatctgg cagagctcag gcagataata gttgacaacg gtattgtatg ccccgtgtcc
    12841 gggacctgca actggaccga ggtgaggcag tttaacctga tgtttgccac tgagataggg
    12901 tcaacctctg acggcgcgat gaagatctac cttcgcccgg agacggcaca gggcatcttt
    12961 gtaaacttcc ttaacgttca gaagaccggc cgcatgaagc tacccttcgg gatagcccag
    13021 ataggcaaag cgttccggaa tgagatcgtg gcacgtcagt tcatcttccg catgcgtgag
    13081 ttcgaacaga tggagatgca gttcttcgtc aaacccggaa cagagcttga gtggtttagc
    13141 cactggaagg aagcaaggat gagatggcac atggccctcg gattcggaga aaacaactac
    13201 cggttccatg accatgacaa gctggcacac tatgccaacg ccgccgctga catagaatac
    13261 agattcccgt tcgggttcaa ggaggttgaa gggatacact cgcgtactga cttcgacctt
    13321 cgtcagcacc aggaatacag cggcaagaag atgcagtatt tcgatcccga gatgaatgaa
    13381 tcatatactc cctatgtgat tgaaacttcc attggtgtcg accgtatgtt cctccaggtg
    13441 gttgccgcct catacttcga agaggatctc accaaggagg atggcacgcc tgacagcagg
    13501 gtggtgcttc gcctgccggc agtactggcc ccggtgaagc tggcggttat gcctctcgtg
    13561 cgcaaggatg gtctcccgga tatttcacga aagattatca gcgacctgaa gttcgatttc
    13621 aactgccagt atgatgaaaa ggactcgata ggcaagcgct accgcagaca ggatgcaatc
    13681 ggcactccgt actgcatcac tattgactac cagacagtgc aggatgaaac ggtaaccatc
    13741 agataccgtg actcaatgga gcaggagcgc gttcctgttt cgcaactcag ggccatcatc
    13801 gctgacaagg tcagcatgca ggaacaactg aaaaagcttt gacatgagag ccgggccggc
    13861 aaaggttctg atcattacct attactggcc gcccagcggc ggagccggag tccagcgctg
    13921 gctcaagttc accaaatacc tcccttcatc cggctggttc cctgtggtcc ttaccgtcga
    13981 cccggaatat gcagcctatc ccttcaggga tgaatccctg tacggcgaga tcccggtgga
    14041 tgtggaggtc catcgtacga aagccctgaa ttttttcgca ctctacaaca gggaccagtc
    14101 taaaatacct catgccggat ttgcctcggg aaaggataag gggattaaaa gcgctgtcag
    14161 cagattcata aggggtaact tcttcatccc tgatccccgc aggggatgga acaggttcgc
    14221 ttttcgcaaa gcctgcgaac ttatcagatc acaaaacatc agtcacataa ttacgaccag
    14281 ccctcctcat tcaacccagc tcatcggcct gaggatgaag cgccggtttc ccggactgaa
    14341 atggatcgct gatctgaggg atccctggac tgacatctat tactatgaca tgttcaggcc
    14401 ttcgctgccg gcaaggatgc tcgacaggaa tctggagaag agagtacttt ccagggctga
    14461 caggatcatt acagtcgggc aggcactggc atcagtcttc gctgcgaagg cagagacggt
    14521 ggctgataaa acagagatac tgcccaacgg atttgacgag gaggacttcc agggggtagc
    14581 gtcatcggtg ccggagagat tcaccataac atatgtcggg actctctccc cggcttatcc
    14641 ggtccggggg ttcattgaag ccgttaagga tatacgcatt gatgggaagc aggtggcact
    14701 tcagtttgtc ggtactgttc ccgaagaggt cagggtgctt ttcccggcag gtgatgaggg
    14761 cggtgctccc cggtttatca gttactgcga gcatcctgag gcaataaggc atatgatgag
    14821 ttccagcctg ctgctgctca taattcctga ccacaggtca aacaggtcaa tcctgaccgg
    14881 aaaaatcttc gaataccttg caacagagaa gcctatcctg tacctcggac ctgaagacgg
    14941 cgatgctgca aggttactgg ccctctgcgg ctaccagggt atcttcggat atgataatgt
    15001 tgaaggcatc agggaattca tcctaagggt tgcagaggga aaaactgtga agcggagcgg
    15061 tcatcacctc gagtattcgc gcagggcgct ggcagtgaag ctgggaaaca tactcgacag
    15121 gctgtagcag ggactgacag tctggttcct gcagggaagc acctctcccg ggttacccgg
    15181 aaacaggcac gcaggttacc gtttccgcct gaaccgatcc attaatgatt cagctactcc
    15241 gttaaggtca tccgaaagct tccagaacca aaccccggca gtgaaaatca ctgtcaccag
    15301 caggcttctt gcaataatgt cagaaatgag cggcatctgc ggtatgaagt accctgccac
    15361 aagtgctgca agaccgacaa gagtcatctt caggtgcaaa atgttgtacg gccagaggcc
    15421 aaaccttttg ccggtcagca aaacgcccag catgctgtag ataatattcg agatcatcga
    15481 agcaagagca gcaccggtta tcccgaacag ggggatgaag atcaggctcg ttcccacgaa
    15541 aaggccgatc aggatgaaca taagccaggt ctgataccgg taaagcgatg aagtactgag
    15601 cactacaccg ttaacacccg cagaaccgct tacaaggttg ccgaagctta tgagtactat
    15661 tactgccgca ctctttgaat atatatccgg cagcaggcgg aatatgttgt caatgttgac
    15721 cagcagaccg atgaagatca gaagaccgac agcatactgg ctaacactgc tgcgcacgta
    15781 catgtcctca attttttcaa ggtcgttctt cttccatgac tcggcaagaa atggaatcga
    15841 tatttttgat acagaccggc cgggcagtgt gatcagcact gcaaaatatg aggctataga
    15901 gtaaattccc acttcggcct cgccgagata ggtattgata aagagcttgt caagggttgt
    15961 cagtgtcacc gtgcttatgc ctgtcagaat gccgaacaat gacaatatta tgatctcgcg
    16021 cttcagatgc ggggtgatga acccgggctc gagcctgagt gagatctcgc ctcttatcag
    16081 aaggctgatg aatatgataa tcagtggcac cccctgggaa accacatacc cgaagacgta
    16141 actgtcaaaa tcaattattc ctgcccagaa caacatgatc agccccagat tcagcactct
    16201 gaaaaggaac tccctgagga atgtcccaag cacagcgtca tacagaacct tattgtagtt
    16261 atccaggaga ttgaacagca ccgttacaag caacagtgca gggagataga tggaatacct
    16321 tgagattaga ggcgagcgct ccaggtttgc ctgctcaaaa tgaggtgcgt aatacttcag
    16381 gaaaaccagc gcagcaatga accctgcaag ggttataaca acagcaagcc cgaggaatcc
    16441 gtgatgcctg ccacggatac cacggaaata ggggaacagc ctgttgatga cgtttgtgaa
    16501 tccgaggctg gagaactgcg ccagcatagt ggcaaacgag agaagcagct gaaccacccc
    16561 gatctctcct gtggtaaata ttttaggtga aaggaggatt atatttacga agccaagcag
    16621 ggctcccagg taagaccagg cggtcccgcg tattgtctgc ctctgaacta tgcccatgtt
    16681 gcttcgccgt tgcggcaaat gtaaaacaat atactcattt gaattaagga acagttggtg
    16741 tatttttgtc ggatgattca aatccgggca tatgacccac taacatcctg caaatgaaac
    16801 cgagtagctt caaatcagca ttgccgcata taattgccgt gctcctcttc actgtggtct
    16861 ccttcgccta tttcttcccg gtactggagg gaaagaagat aaatgcccat gacaccaaag
    16921 tgtacgaggg atcttcaaag gagataagcg attaccgcga gaaacacggc ggtgaaccgc
    16981 tatggaccaa ctctatgttc agcgggatgc ctgcttacat gatctcggtg aaatacccgg
    17041 gaaacctgtt caaacacctc gacaacttcc ttaaggtctt taaaacacct gtggcagcgc
    17101 tcttcctctc aatggccggc ttctacatca tgctgctcct tttcggggtc aacccatggc
    17161 tggcgatggc cggggcaatt gcatacggct tctcttcctt tctctttgcc agcctctcag
    17221 cgggtcataa cacaaaagcc tatgccatgg cctacatggc gcctgtcatt ggctcggtgg
    17281 tctactcctt ccggaggaat accatgacgg gagcctcact gtttgcactc ttcctggcgc
    17341 tacagataat ggcaaaccac gtccagataa catactatac cggcatcatc gtgcttgtat
    17401 tcggaatcta tgagctgatc cacgctgtaa aaagcaggat gatcccatcc ttccttaaat
    17461 cactgggagt acttgtggtg gcagcgctga ttgccttcag cgtcaatttt gcatcgctct
    17521 attccacatg ggaatacagc aaggagtcaa cccggggcaa aagtgaactc acaacggccg
    17581 caggtaccaa tacgaagggc ctcgacccgg agtatattac acaatggagc tatgggatag
    17641 gtgaatcaat gacgttcctg atacccaact tcagaggcgg cgcaacggca cccttcgaca
    17701 gcaactcgga gacggtaaag gctctgagga agaacaacat gggccaggca cagggacaac
    17761 tgtacaggta ctggggcaaa caacccaaca caagcggacc tgtctacttc ggggcggtgg
    17821 tgctattcct cttcgtgctc ggactgatgc tgatcaaagg ccgtgacaag tggtggatac
    17881 tgactgcagc gctgctctcc ctgctcctct cttggggcaa aaacctcatg ccactgacca
    17941 cccttttcat cgatttcttc cctggatacg acaagttcag agcggtgtca atgacactgg
    18001 tcatggctgg cgtgctggtg ccgctgctgg gtgtgctggc cctaagggag atttccgaag
    18061 gaaccgtcag cagggaaaag gctgccagat cagtgcttat tgccgccatg gttaccggcg
    18121 gactggcttt aatcttcttc cttataccgg gtctggcagg atcattcctg agacctgacg
    18181 aaatcgcact gccagattct gccgcatgga tcaaggatgc tatggcaacc gacaggaaga
    18241 tgatgattcg cacagatgcc ctcagatcag ctgtatttat tgccggcgca gcagccgtca
    18301 tatggtttta cctgaagaac aaactcaaac ccgcatatgc acttatgggt ctgacggtgc
    18361 ttttcctgat tgaccaggtt ccggttgatg ccaggtttct cggcagctcc agttttgaga
    18421 caaaaagggc atctgcaaac tcctttgccc catccgaagc agacaagatt atcctgggcg
    18481 acgagggtga attcagggtg cttaacctgg ccgtttcggt tttcaatgac gcttccacat
    18541 cataccacca ccattccata ggaggttaca gcggggcgaa gatgaagagg tatcaggaac
    18601 ttattgagac aagcctgtca gacgacataa attccctgat aacgaatctg aggacagcaa
    18661 cgaccatgga ggaagcagaa ggagtgttgg gaagcctggg cacacttaac atgcttaaca
    18721 ccaagtatat tatacttgac cccggatcag ccccactggt gaacaggcat gccgccggta
    18781 acgcctggct ggtcaacagg gttacacttg ctgagaacgc tgatgctgaa ctgcaggcag
    18841 ttaaaaccat taaccccagg gaggaggctg ttgttgacag gcgctttgct gagttgatca
    18901 gggttgctga aactgccggg gcgcccgggg atacaatatt cctgacctcc tatgagccca
    18961 acctgctgac ataccaggca gagttgtcat cagacagggt ggcgatattt tccgagatct
    19021 tttacaagta cggatggaag gcatctgtag atgacaagcc ggctgatcat ttcagggcca
    19081 attatgtcct caggggaatg acccttcctg ccggaagcca tactgtcaca ttcaggtttg
    19141 acccggaatc gtacaggacc ggcaacaggg tgtctcttgc aggttctgtt ttacttctgg
    19201 cgtttcttct gctggctgcc ctcccgctgc tgaaaaagag gagccgctga tggctgaacg
    19261 acttatctgc cctgcatgcg gatctgcaga catcgccggt ctgtttcatg caaaggatca
    19321 tctggtcagc agcagggagt tcctcattat gcgatgcaat gcctgcggta tgggatggac
    19381 agctgaccct cccccggaag aagaggcagg caacttttat gtctctgagg aatatatctc
    19441 tcatactgac aggaaacaga gcatttccga ctacatctat caccttgccc gcagctttat
    19501 gcttggccga aagcaaaggc tggttgcgtc agctgtcggt aaagagaccg gatcactggt
    19561 tgatatcggg agcggtacag gttattttgc cgcatacatg aagaagcggg gatggcaggt
    19621 tacgggcatc gagatcagcg ataatgccag gaaatactcg gtagcaaggt ttggaatcag
    19681 ggcggtgact cctgcggaga taaagggcat gccgcccgac tcggctgatt gcatcacact
    19741 atggcatgtg cttgagcacc tctatgaacc ggatctgtgg atggacgagg taaaccgtat
    19801 cctcagggat gacggcaagt gtattatagc gcttcccaac ttcgcatcat cagattcagc
    19861 ccggttcggg aaccggtggg cggctctcga cgtgcccagg catctgtggc atttcacacc
    19921 ggcagctttt gttaatttcg ccgacaggca cggacttgca tgcgaaaaga tttcggcctt
    19981 gccacttgat cttttttata tttcctacct gagctacagg aacgaaagca aatccatgcc
    20041 actgataagg ggagccgtaa caggcctttt tatcgctctg cgaaacattt tccgtaaaaa
    20101 gaaggcaagt tcactggttt ttgtgatttc aaaaaagaaa tcctagtacc tgtcaagcca
    20161 gcggtatttc ctcggtgtat cgccgaacct ggctgccaca atgaactcat gtgacccgta
    20221 ggtggacatc tggatggaat taagggtaaa atcaaaggag tacccaaaga agagcctgtc
    20281 tctcctgaca cccacattgg ctatgatagc gctgccggtc ctgaaggtag ccccaaccca
    20341 gagtttgttg tcatatgagt aggtaacacc aatatctgcc tggggcctga cctgctcaga
    20401 catctttata agcagtgatg gctgaatcag cgttttgttg tcaggctcaa agtcatagtt
    20461 tccgaagagg taataggtcc tctgtatctt caggtccctg tatgcctggc ttcctgcctt
    20521 gatgaacccc tccatcaact cctgtgcgga gaatccgatg gcatatttcc tgttcagaag
    20581 atatgcaccg aaattgaagt cgggaacaaa tattcccttc ctgaaatcag tgttcagcca
    20641 tggctcatca ggatcttcga actgtatctg tctctggtct atcttgtagt gatagcccgt
    20701 aaaggcgagc cctaaggaaa gctgtgtccc tccctgcaac cagatatgat atgcatatga
    20761 ggtggagaat ccggtcctcc ggaccaggcc gttaatatca gaaaagaccg ttcccccgat
    20821 accaacgcgg ccctcggtat gtggcttgaa aatcttcttg tcaaaaacat tctgacgtat
    20881 gaggtacttc tgcttcagca gcctgccgtt aaagctgagc gaatatgtct gtggcgctcc
    20941 ctggtatcct gcccattgct gccttgtggt caggttaagg ctggtgtaac cgtcactgcc
    21001 agccactgca ggattgatga gatacttgtt gaacagatat tggctgtaaa ttggcagttg
    21061 ctgggcgtca agcactgatg taatcatgat tgcagccagt atgatcaatc cccttttcac
    21121 cttatgtact ccttcacttc tgcacataat ttacctgata atggtcacat gaccgacaat
    21181 cggtttctct ccgttgtgga ggtctatcgc gtagtggtat gaatccatcg ggagcacctt
    21241 gccgtttgag gcgcggccgt cccatggatc cggatatcct ttttctgatt tccatactat
    21301 ctcgccccac ctgttgagaa tgatcacttc ggcttcgttg tagaatgaaa tccttgtgat
    21361 gttccagaac tcattgatcc cgtcgccgtc aggcgagaag gcgttcggta tgcccacaca
    21421 aatgtcattt gtcggcttaa gtaacaggga atctgaaagt acgcatccgt taaagtctcg
    21481 tgcttcaacc gtgtaaagcc cctgtaccag gccaacagcc tcctgggtgg tctgcccgtc
    21541 gctccatgtg aaggtgtatg caccctcacc accggtgaca cctgcaaaga tgcttccgtc
    21601 attgctttca ggacagtaag gcgagatcag ggcaaagctg atccgcagcg aatcgggttc
    21661 agtgagcgtc agtgaggtgt cagcaatgca gccattggcg tctgtaagga ttacctcatg
    21721 atagcctgcc gtaaggttat ccctggcagc tccggaaagt ccgtctgacc agatatattc
    21781 aggtgtgccg gcggcattca ccgctgttac attaaccctg ccggaggaag ctccgttgca
    21841 gttgatatta aagctgccgc cgttgctaag gccaatactc agagtcatac tcagttgccc
    21901 gggtgaaacc agtgtaatat cctgtgtcac agaacagagg tgcgcgtctg ttacaatcac
    21961 cgagtaggtt ccttctctaa gagagtagat cgaatcagta gtcgcggtaa accctgcagg
    22021 tccgctccaa gaatagctat acggagccgt gccggtctga ggaatgatcc ggagccatcc
    22081 gtcacttttc ccgagacagc ttatgctgaa tccgttataa tctgaaatac gtggttcgag
    22141 cgagattggt tcaggcaggc tgagaaaggt gtctgaggca atactgcatc cgtatgaatc
    22201 ggtgacagtg attgtataag tgccttctgt cagtgctgag atgtcttcaa gggatgatgt
    22261 aaagcctgcg gggccggtcc aggaataaga atatggcgga cgcccgccgc tgactgtaag
    22321 gtcaattgaa ccgtcattgt aagccggagc atttaagcag gttatgtcag acactgcaag
    22381 agacaggctt aatgagtcag gttcggtgat ctccactgtc cttgtgttga cacaaccgtt
    22441 cacatcggta acctgcagca gatatgtccc ggctttgaca gtcaggtcct ccagattgct
    22501 ggcatatgac tgatctgcgg attttgtcca ctggtaagaa taggttcccg caccgcttcc
    22561 gccgctaacg gtgatatcaa ttgtaccatc accgccattg cagcctatat tgaatgcatt
    22621 gtcaggtgta tgggatgttg atactgcaat gctcaatgaa tcaggttcat caagaatata
    22681 ggtatactcc cggtgacaca tgttgccatc agtaactgta agattatagg ttccagcacc
    22741 gatccctgtc agtgattggg tgtttgatgt aaagccgtca ggacccgtcc acgcataacc
    22801 gtaagctcct gtgccaccgt caaactgcag gttgataaat ccgtcactgc caccatagca
    22861 cgagatctcg taatttccgt cattgctaag tgagacttct tcatcaataa ggtcaatgcc
    22921 gtcaggctcg gtcatagtct cggtaaagtt gaatatgcag ccctggctgt ctgttacagt
    22981 gagagtatat gtaccggccg gaatattgac aagaagactg tctgttgggc ttccgctgat
    23041 gattcctccg ttggaggcag accacaggta agtatatgga gccctgctgc ttacaaatac
    23101 agtatcagcg ccttccatat aatagctgta tatttcactt actctgatgg ttccgtcact
    23161 gtagctctcg catgaaatat tgtcgtcagc atatagtgac ttttcaaagc cggcgaaggt
    23221 gacaggtgca tcgtacagcg ttctcggcag ttcctccctg catccattgg cgtccacaac
    23281 ggtaagcaaa tattcacctg gtttcaggtt gtcaagagag aagaaagtgc cctcgggcgg
    23341 catccatccc tggtacacgg tgtcagcgct gttccagaca aggctgtagt agtaaggagg
    23401 tgcatgccct tctgtcaatg caacaaccat tcttgcatcc tcagcgccgg ggcatgagat
    23461 aaactggcca aaatagagtg acacctcaat gcttggggcc aggaaattaa gtgcgtcaga
    23521 gctggagcaa ccgaggctgt cagtgaccgt tgccgtataa tatccctggc tgcactcctc
    23581 tgcgatgaac atgttgtatg cagcctcttc ccagtaatca ggccctgtcc atgccaccca
    23641 caatgaatca aatccacgtg cataaattac ctgcagagct ccgtcctgac cgccatcaca
    23701 tgtgatagag tcgaggatct caagtgattg aagcggatga gggtgaacct ttatctcgga
    23761 tattaccgga ggtccgtaag gacaacccgg acccgtcacc ttgggtgtaa tccggaagaa
    23821 caccgactgc atagtgtcac tttcattggt ataggggaac tgcagatgag tcccgggtgc
    23881 ctggctgttc agcgcggttc tgttccctgc gacaacagca gtggaggctg tggaagtgat
    23941 tgtatagttg aattcattta ttccggagct catgactgtg ggtgacagaa gcacgacgtc
    24001 cgtggtgcca ccgtaacata tgtgcggagc atagttctcc ggcgttgccc tgggggtagg
    24061 attgacccat accctgactg tatccctgat gccgtagcac ttctgattgc cactgacatc
    24121 aagtgttgca ggagctacaa cataggtgac aagcctggct gtgtctccgc tgttgctgag
    24181 cgcttccgta atgatatcgg taacaatcag cccggtcctg tcggtgaatc cggttatctc
    24241 aggatagctg ttaatggctt caacgttgaa ttccaggccg ttgtatgcag ttgttggcgt
    24301 ggcgatctgg atgttcacgc tttcaccatt gcaaagctcg ttatctgaca gggtcagggc
    24361 gaccctggct gtcggttcaa catggacagc gattattacc tctactccat catggcaggt
    24421 cggatcaccg gttttgccct gtatgtaagg tctgaatctg tagattgctg tctggtagct
    24481 gtcggagtta ttggtgagca ggtcagaaat atttattatc tggttctg
//
LOCUS       DAOPXU010000036        24375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_2060_length_24375_cov_12.845148, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000036 DAOPXU010000000
VERSION     DAOPXU010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24375
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2060_length_24375_cov_12.845148"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..241
                     /gene="infA"
                     /locus_tag="PKK03_05335"
     CDS             23..241
                     /gene="infA"
                     /locus_tag="PKK03_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNX83854.1"
                     /translation="MAKQPSIEIDGTIIEALSNAMFRVRLENGHIITAHISGKMRMHY
                     IKILPGDKVKVEMSPYDLTKGRITFRFK"
     gene            256..372
                     /gene="ykgO"
                     /locus_tag="PKK03_05340"
     CDS             256..372
                     /gene="ykgO"
                     /locus_tag="PKK03_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HNX83855.1"
                     /translation="MKVRASVKKRSADCKIVRRNGRLYVINKKNPKFKQRQG"
     gene            410..787
                     /gene="rpsM"
                     /locus_tag="PKK03_05345"
     CDS             410..787
                     /gene="rpsM"
                     /locus_tag="PKK03_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNX83856.1"
                     /translation="MARINGVDLPRNKQGEVALTYIYGVGRSTAQKVLDKAGVDRQMK
                     VEEWNDDNINAIRQILSENYKLEGELRSETQLNIKRLMDIGCYRGIRHRIGLPVRGQS
                     TKNNARTRKGRKKTVANKKKATK"
     gene            795..1184
                     /gene="rpsK"
                     /locus_tag="PKK03_05350"
     CDS             795..1184
                     /gene="rpsK"
                     /locus_tag="PKK03_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNX83857.1"
                     /translation="MAKKTGTLKKKVVKVEPVGQAHVHSSFNNIIISLTNSTGQVISW
                     ASAGKMGFKGSKKNTPYAAQMAAEECTKVAFELGLRKVKVYVKGPGSGRESAIRAISN
                     SGVEVTEIVDVTPMPHNGCRPPSRRRV"
     gene            1229..1837
                     /gene="rpsD"
                     /locus_tag="PKK03_05355"
     CDS             1229..1837
                     /gene="rpsD"
                     /locus_tag="PKK03_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNX83858.1"
                     /translation="MARYTGPRSRIARKFGEPIFGPDKYLDRKNFPPGQHGLTKKRKK
                     TSEYGVQLREKQKVKYTYGLLERQFRNTFEKAVAASGVTGLVLLQLLESRLDNVVYRL
                     GIAPSRPAARQLVSHRHITINGNVVNIPSMMLRPGDIIGVREKSKSLEIITECLGANR
                     RSKLAWLEWDDTQMAGKYMSVPERTEIPEDIKEQLIVELYSK"
     gene            1856..2848
                     /locus_tag="PKK03_05360"
     CDS             1856..2848
                     /locus_tag="PKK03_05360"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNX83859.1"
                     /translation="MAILAFQKPDKVILLEADDQYGKFEFRPLEPGYGITVGNALRRI
                     LLSSLEGFAITTVKIEGVDHEFSTITGVMEDVTEIILNLKQIRFRRMVEDIDTEKIVV
                     KVSGQEAFKAGDLNNSLSSFEVLNPDLVICRMEPDVKLQFEINISKGRGYVPAEENQP
                     AEAEFGLIAIDSIFTPVRNVKYSIENYRVEQKTDYEKLIIEITTDGSIHPKDALKEAA
                     KILIYHFMLFSEEKITIDYEDKMANEEFDEEILHMRQLLKTKLVDLDLSVRALNCLKA
                     AEVETLGDLVKFNKNDLLKFRNFGKKSLTELDELLETMSLSFGMDVAKYKLDKD"
     gene            2871..3389
                     /gene="rplQ"
                     /locus_tag="PKK03_05365"
     CDS             2871..3389
                     /gene="rplQ"
                     /locus_tag="PKK03_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNX83860.1"
                     /translation="MRHLNAINHLGRKSADRKAMMANLASSLILHKRISTTTAKARAL
                     RTYVEPLITKSKDDSTHSRRVVFSYLENKGAVAELFREVAPKIASRPGGYTRILKTGS
                     RIGDAAEMCIIELVDFNENMLAGEGAKAKASRRRRSPKKKTAETAAVTETNAKAETPA
                     EEKAPEAEESKE"
     gene            3484..4761
                     /gene="eno"
                     /locus_tag="PKK03_05370"
     CDS             3484..4761
                     /gene="eno"
                     /locus_tag="PKK03_05370"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNX83861.1"
                     /translation="MSQIVSVHAREILDSRGNPTIEVEVITSSGASGRAAVPSGASTG
                     ENEALELRDGDKSRYLGKGVLKAVHNVNNVIAEDLMGMDVTNQIAIDQKMIDLDGTPT
                     KSTLGANAILGVSLACAKAAAAYHGLPLYRYIGGTNARVLPIPMMNIINGGAHSDAPI
                     AFQEFMIRPKGAGTFAEGLRMGAEVFHSLKKVLKERGLSTAVGDEGGFAPVLKGTEDA
                     LESIIKAITAAGYKPGVDVMIALDCAASSFFKDGVYDYTIFEGKDAKKLTPAQQADYL
                     TALVEKYPIIDSIEDGMSEGDWEGWKLHTQQLGSKIQIVGDDLFVTNVEYLEKGISLG
                     CANSILIKLNQIGTLTETLNCIEMAHRAGYTTVTSHRSGETEDSTIADVAVATNSGQI
                     KTGSLSRTDRIAKYNQLLRIEEELGANAVYGCE"
     gene            4841..6730
                     /locus_tag="PKK03_05375"
     CDS             4841..6730
                     /locus_tag="PKK03_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HNX83862.1"
                     /translation="MSFNGLSQFVSFLESRGELVRVTSPVNPVLEITEIADRLVKSGG
                     KAVLFENTGTAFPVLINAFASDTRMAAAMGRNSLDDAGDELFTLVESLTQARGLAGKL
                     ARLPGMLRLLTLSPKRRSGRGECQEVVEQVPDIGRLPILKCWPHDGERFVTLPLVHTR
                     HPETGKPNLGMYRMQVMGRDMTGMHWHRHKTGARHFEAWKKRGERMPVTVTLGGDPVY
                     TYAATAPLPEDISEYLLAAFLRRKRVSLVKCLTNDLWVPADSDFVIEGYVDTAEAPVT
                     EGPFGDHTGFYSLADLYPVFHVTCITHRKNAVYPATIVGVPPMEDAWMGKATEKIFFA
                     PIKLAIAPEIRELHMPVSGVAHNLVIVSIEKTYPGQGMKVLSALFGAGQMMLSKYIIV
                     VSGNVHVTDYRAVAEEVFANVRFDSDLLLTRGPLDVLDHSSDRFSMGGKLGIDATVKL
                     SEERTDEYHSVPLLLLTGEQFNEKVISGMKTLADYALPVAILTITKPEEGLDMHRLAA
                     SLPASLTTPGTVIIAVDEGADADDPAMLTWLVTGNSDPARDLYHLEGGAIFIDATSKT
                     RSLPAFPRDWPNVVCSDPETIELVNTRWPEYGLGPFIESPSLRLLPLLKPGEASVETV
                     NSTAG"
     gene            6727..7707
                     /locus_tag="PKK03_05380"
     CDS             6727..7707
                     /locus_tag="PKK03_05380"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNX83863.1"
                     /translation="MTGLPSAALLTAFVQGLRDSPALNYLIVIFIIAALVYLIVLQFW
                     VLLRTKKHLREIQGAYSEIEVQRSELQLRNKDLTDSLNYARRIQAALLPAEHHIRRIF
                     PDHFIYYRPKHIVSGDFYWFSERDEKYFIAAADCTGHGVPGALMSMIGLELIQKIINE
                     MKVDDTDQVLITMNRELESAFFKEESGKALIKDGIEMGICVIDRNTKVMEFSGAFLPV
                     YIVRDDKLIEIKGDKKNVVQSFAMVSFNRSTFTLQEGDILYLFSDGYADQFGGPENKK
                     FMYRRLRHILLTISKYPLADQQRILDETIGSWMEGHDQIDDMMILGVKPF"
     gene            complement(7710..9041)
                     /locus_tag="PKK03_05385"
     CDS             complement(7710..9041)
                     /locus_tag="PKK03_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX83864.1"
                     /translation="MPNRSRPSAIPHSDLMTRWTRDRILLPVAVALLLVVIALVTALA
                     GLVYMPIAAGVLALVPLILAIAELRKLIADNREILRELASIAEKNEEVINSFSHKIRE
                     PLNNLVLLGNMLSETTSSPKQQELVETFIASTGSMVEVVNELTMETAGNITFERRGEI
                     RFRLGPIIRDTIGLMKLSRRHDQSVVVYDDHGEGEREYRGDPIIIKQILIDLLSAAIS
                     GPAGKVTIKIQAREAGEREGAHKVEFTLSSNNQAVFIGEGRQPYPLAGRLIRDCGGNW
                     QESTSGTGSYFVFYIFLRRADKVIRPAAASTLVREMETEKSHKELKELNILLVEDNPI
                     NQRITQLMLEPLVKRLEVARNGKEALDMFGTSRYDLILMDVQMPVMNGLVAAEKIRGL
                     EMSSSGHVPIIAITANAMLGDREQCLAVGMDDYISKPIEPEVLIEKITALI"
     gene            9182..9928
                     /locus_tag="PKK03_05390"
     CDS             9182..9928
                     /locus_tag="PKK03_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83865.1"
                     /translation="MKKCLLTLIAVLVATGFTRAQQGDTLVQYTPDFKFRDGIYLNFD
                     MVKTNSPIPKAKLFTSVDYNDKDFFDQVISSEKIYFYDNMGVRQEIEKDAIWGFARNG
                     VLYAKVQNNFNRITFFGSIIHFVADVTTQTGYNPYGYYDPYYSPYRSFYSPYYYSPYS
                     SRYYDPYYYGGSPYSRNTSRTDLEMFMIDFETGKTYEYDLENLEALLMRDPELYEEFV
                     SMRNKNQKKMMFVYLRKYNERHPILLPSGK"
     gene            9936..10787
                     /locus_tag="PKK03_05395"
     CDS             9936..10787
                     /locus_tag="PKK03_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83866.1"
                     /translation="MKKFPLLALALLLASAISAQQRPYPTFDDAAKFKKSVTCVVRED
                     DPFSFYNAEIKAAVDKYWKVTPVKYITTEEFNVMRNDPAYSFLVLTITNFSNDKSGST
                     YDFLNLLLGADVDELDQLPEFCAIPLCFSGAPEEEYSYKLGLIIRFMEYHAELVMKNP
                     TLSALRYLKYYNKNVPEIKDKTILVRQADLAPEVDSPERIAAYYPYKVRIVDEEEIIR
                     AIDEKQKDVLIVHKVGPEGVKRTGTCMKTLIGTDDAIMYYYDSHMVDSKNASGLLIAD
                     LKRLARF"
     gene            10832..11188
                     /locus_tag="PKK03_05400"
     CDS             10832..11188
                     /locus_tag="PKK03_05400"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNX83867.1"
                     /translation="MEETKTYTQSERDWAMFCHLSTFAGYFFPFGGIIGPLICWVSRK
                     DQSEWVDTNGKSALNFQISMLLYTILLIPLCFIIVGIPLLVALGILEVVCVIVASIRA
                     AKGEAFKYPISIPFIQ"
     gene            11375..12751
                     /gene="hisS"
                     /locus_tag="PKK03_05405"
     CDS             11375..12751
                     /gene="hisS"
                     /locus_tag="PKK03_05405"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNX83868.1"
                     /translation="MAQKTSIPKGTRDFSPAEMLRRNHIFGIIRKIFSLYGYLPIETP
                     AMENLSTLLGKYGEEGDRLIFRILNSGNFLAGVSPSVLSSADTSSVIRQISEKGLRYD
                     LTVPFARFVVQHRDEITFPFRRYQIQPVWRADKPQKGRYREFFQCDVDVIGSDSLLNE
                     AELVRIIDRIFSELGINIIIKINNRKILAGIAEIAGAGERITDITVAIDKIEKIGREG
                     VRQELAQRGIAPGVIARLEPVIDLSGSTEQKLTELRNVMAGSVSGVKGIGEMEELTGY
                     LAIEPVKAPVELDLSLARGLNYYTGTIIEVKAADLPFGSICGGGRYDDLTGIFGMPGV
                     SGVGVSFGADRIYDVMMQLSLFPEQTGSSTEVLVINFGSGELPALMKVAGELREAGIA
                     TELYPDPAKLKKQFAYADSSMIPLVVIAGSNEIAAGQATVKNMKTGEQTAVPLPELPL
                     FIRRVMTS"
     gene            complement(12756..13295)
                     /locus_tag="PKK03_05410"
     CDS             complement(12756..13295)
                     /locus_tag="PKK03_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83869.1"
                     /translation="MKRIITTIAAVLLTGVTAMTLRAQGQGMGQGAMQGDGEKLTAYK
                     IAFFTKNLDLTPAEAEKFWPVYNDYSARKSKLQADRLLLMRYAAQNQANMTEDEVAST
                     ADNLTRSFADEAAMVVSFNENLKKVLPPAKVIRLYQVENQYKQQLLRELNQRRQGPAQ
                     QGQGMRRSSGQSGPAPLTE"
     gene            complement(13300..13830)
                     /locus_tag="PKK03_05415"
     CDS             complement(13300..13830)
                     /locus_tag="PKK03_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83870.1"
                     /translation="MKLSEEKGKENPFSVPEDYFDTLADRTMAAINEQAGKGKDWAVI
                     KVVAGKGEDWATITEGDGKAAIREEKLRKPGMIVRLKPFLALAAAILGFAILASALVR
                     LINVDRPDRVSDSGNSLYADLAVEEIGTYMLEDELNMTDPVIPEITGETISSETIIDY
                     LMTEDINLDDIYELLL"
     gene            complement(13864..14406)
                     /locus_tag="PKK03_05420"
     CDS             complement(13864..14406)
                     /locus_tag="PKK03_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNX83871.1"
                     /translation="MSQSTEDDIRKALAADTEKAFRMIIDLYGRKLYWHIRRLVITHD
                     DADDALQNTFLNAWKNIGSFRSESSLLTWLWAIATNEALNIIRRRGRENSVALDDVEE
                     ILASSAEGSTWFDGDAATVRLQNAILKLPEKQRAVFNMRYFEEMTYEDMSAVTGTSVG
                     ALKASYHHAVKKIEQFLDRD"
     gene            14519..15607
                     /gene="ychF"
                     /locus_tag="PKK03_05425"
     CDS             14519..15607
                     /gene="ychF"
                     /locus_tag="PKK03_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNX83872.1"
                     /translation="MALKCGIIGITSVGKTTIFNCISNTRGETNTYGTGVKANLGVIQ
                     VPDKRLYELEKFQPTERIVHTTVDIVDIPGLVRGQGDSSGARLLGEIRNTDALIHVLR
                     CFDDDNLPHIDGSVNPVRDLENVEFELQVKDVESVVKKIQRLERAAKTGDKEAKHGVE
                     ILNLYREHLEGFQNASSLELKPEEKKVVDDIFLLTMKPVLYVCNVDEASAVDGNRYVE
                     DVRSFLAPRSTEPLVIAGALEAEIANLTEEADRLEFLGASGLTEPGVNKLVRAAYRML
                     NLQTFFTVGPKEIRAWTITAGMTAPLAAGVIHSDLERGFIRAEVMKYDDFIRLGSEHA
                     CREAGKLMVEGKAYVVGDGDILHIRFNV"
     gene            15616..17337
                     /locus_tag="PKK03_05430"
     CDS             15616..17337
                     /locus_tag="PKK03_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNX83873.1"
                     /translation="MKLRSVIPVLLLLVSMQAVCGQESPAGDDIRNAVSRYGQAEVMI
                     SYPGYDAMTRLAARFSVSSCDGLTALLSLSSRDVAEFIAEAIPYRLIVPEERKGFYTA
                     SSVAEAMLWQSYPTWKHYDTIMHKIAGHWPDLCRLDTIGFSVQGRAVLALKISDNPEE
                     DEPEPEVMISSSIHGDETAGFVLLMRLAEYLASGGGGAPGSGLASELVSELEIWINPL
                     ANPDGMYRNGDTLTYPVRANSNGYDLNRNFPDPEVGTPPPLQKETLDMIRFMEERRFT
                     LSANLHSGAEVVNYPWDKWTRDHADDDWFNAISRRYADTVHLHSESGYMTFLDNGVTK
                     GWKWYLVRGGRQDYVTWGLGGREVTIELDDTKMTPGSNLELMWEWNHRSLLRYIAGAM
                     TGVRGTVTDAETGDPLSAKIFIAGHDRDSSHVWSDTTTGFYTRLLPPGTWNLTFSCPG
                     YKPYTLTADLTSSVPLQVSNIQLEKGNHDYPDPPESGLLVFPNPSDGNFSIMPPVAVT
                     GEVTITVTSTSGALMKHYTTTAQPGIPVECKFTGLPAGIFIISVSREPNGPMVRGKAV
                     ITRLINR"
     gene            17425..19074
                     /locus_tag="PKK03_05435"
     CDS             17425..19074
                     /locus_tag="PKK03_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNX83874.1"
                     /translation="MKRVITVLYLLILTFSVTAQSAYIPPDKPKLVIGIIVEQLRYDQ
                     LERIWEILPDNGLKRMVSEGTYYRNASIDYLSTQAAPGFATISTGASPSAHGITSDSW
                     FHPFNDEMIYCVQDATASPVGGSFETGLFSPVNLLSSTFADELQMASCGGSKVYGIGM
                     KEMAAIITAGHAADGAFWFDDRTGTWMSSTYFIKSLPPWLMDLNAMMMPQQLLNQQWM
                     PLHDPATYPGCQPDSSSLERGFNGQTWFPYDLKALSTKGKLLNVTRDFSVLRDTPFAD
                     EFTTELALRLIDNEQLGQDDITDYLAITYSSTDYVGHRFGPSSVEASDALVRLDRNIA
                     RLVEKIDKSLGKKNVLVYLVSAHGVSEIPAVLEQSRVPAGYFRLNQSLQLLRSYLNAV
                     YGQGDWVRGFFDSQIYLNRGLIEGAKINLEEMQKRVARFMVQFSGISAAVPTSAFEMS
                     DFSGGLLRKMSNNYSQQRSGDVMIALNPGWVEKTDNVTGHNSPWEYDSHVPMIWFGWT
                     ASRVSVTRSVSTRDIATTLSVLCKVPLPNASSGDPLHELFR"
     gene            complement(19104..19844)
                     /locus_tag="PKK03_05440"
     CDS             complement(19104..19844)
                     /locus_tag="PKK03_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HNX83875.1"
                     /translation="MIVEIPWKKRKLVAQLIDPDRAGEETLVNAARLADEAGVDIFLA
                     GGSLTSVPVADVIKRLKQLSSIPVVLFPGNLTQLTAGADAVFMLSLISGRNPELLIGS
                     HVIAAPVLKNMRERVIPVGYMLIDCGGGTSVEYMSQTSPIPSGKNDIAVATALAGEML
                     GMKAIYLEGGSGAARPVDPSMIKAVRDELTIPLITGGGIDSAVKVEMAFDAGADMVVI
                     GNGCEQNPALIKGACLVRDSFNRGAVII"
     gene            complement(19834..20478)
                     /locus_tag="PKK03_05445"
     CDS             complement(19834..20478)
                     /locus_tag="PKK03_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNX83876.1"
                     /translation="MGCITKHYLNKETVIGVWKIEEDIDTLLSMVEFSPEDRKRYEFF
                     RSNSRKIEFLSVRALLAEMLGSEARIVYNKNNKPFIKDGSHFISITHSNKLTAILLSK
                     TERVGIDLEYMRVNINAFASKFMNKREKVTRRWADRTYHLYIHWCAKEAIYKICDKEG
                     INIVNDITIEPFVVKESGEIRGSVENERISDRFTLYYSKYDNYTIVWTKKKHDS"
     gene            complement(20482..21054)
                     /gene="gldD"
                     /locus_tag="PKK03_05450"
     CDS             complement(20482..21054)
                     /gene="gldD"
                     /locus_tag="PKK03_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HNX83877.1"
                     /translation="MRKTIAAALVASALILAGCDHPTVPKPKGYFRIDLPQKSYIHFD
                     SPCSYSIEYPHYAVVNLHPAAATDTCWMDIEFPSLKAKIHLTYFDIHDNLASLTEMSR
                     EMAYKHTIRAEAIDEKLWADESTGVYGMLYDLKGNTASAVQFFVTDSTRHYLRGSLYF
                     TAQPNEDSLMPVISFIREDIIHLIETLNWR"
     gene            complement(21051..22379)
                     /gene="gldE"
                     /locus_tag="PKK03_05455"
     CDS             complement(21051..22379)
                     /gene="gldE"
                     /locus_tag="PKK03_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HNX83878.1"
                     /translation="METLPAGSLTGIMALAAVTGSPVALGTEIIVLILLIIASAFISG
                     SEVAYFSLTPGDMEELQHRRGRRQSAAMKLLAKPDRLLSTILVTNNVINIAIIILAAF
                     ISSRLFDLSNNPVLSFIIEVVVITFILLLFCEIIPKVIASRSQLKFALMMAPALTFTA
                     KLFRPLASLLAYSSFFVGRRQKSRDAGLSIDDLSVALDLTSSEIKDDKDLLKGVVRFG
                     NTSASAIMCPRMDVTALDISKGFHAIIHEVIESGFSRIPVYSETFDHVRGILFVKDLL
                     PHLGKPDNFRWQSLIRPAYVVPETKKISELLKEFQAKKIHMAIVVDEYGGTSGLVTLE
                     DVLEEIVGEITDETDEEVPLYKQTGEGKWVFDGKILLNDFYRITGLEDDPFGEVRGDS
                     ETLAGLLLELLGEIPVKGREITAGGFRFTIESIEKRRIREISAEKKGEGI"
     gene            complement(22399..22899)
                     /gene="ssb"
                     /locus_tag="PKK03_05460"
     CDS             complement(22399..22899)
                     /gene="ssb"
                     /locus_tag="PKK03_05460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNX83879.1"
                     /translation="MSFNKVILVGNVGKDPEIRRFENNIKASFSLATSETYTPRGGEK
                     VTQTEWHNIVAWRRLAELSENYIRKGSQILVEGKLRYRSYDDRDGNKRYIVEVEADVI
                     QLLGRKPDNAGQPGQGYQGNQGYQNNNQGSNHGYSAPSQPSSPSAGEGPVEDLSSHDT
                     ADDLPF"
     gene            23174..23461
                     /locus_tag="PKK03_05465"
     CDS             23174..23461
                     /locus_tag="PKK03_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNX83880.1"
                     /translation="MTKADIVNEIAKNTGIEKVTVQKTVEAFMESVKDSMVAGNNVYL
                     RGFGSFIVKKRAKKTARNISKNTTIIIPAHNIPAFKPAKTFINKVKSHVKK"
     gene            23663..>24375
                     /locus_tag="PKK03_05470"
     CDS             23663..>24375
                     /locus_tag="PKK03_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNX83881.1"
                     /translation="MNKDLIISVNDAEISIALLEDKQLVELNKEKRNVKFSVGDIYLG
                     KVKKIMPGLNAAFINVGFEKDAFLHYLDLGAQFRTLHRYYQLATQNKARMTTVSKFKP
                     EADIDKDGKIADVLTSGQTVIVQITKEPISTKGPRLASEVSIAGRNLVLMPFSDKVSV
                     SSKIENADERNRLKLLIQSIKPKNYGVIVRTVAEGKKVAALDAELKELINRWETALSG
                     VHRDVKSPRLLLGEMNRTTT"
BASE COUNT         6179 a         6362 c         6174 g         5660 t
ORIGIN      
        1 gtattaacaa agaggtaagt atatggccaa acaaccatca atagagattg acggaacgat
       61 catcgaggcg ctctcaaacg cgatgttcag ggtcaggctc gaaaacggac atatcatcac
      121 cgcccatatc tccgggaaga tgaggatgca ctatattaaa atcctccccg gtgacaaggt
      181 gaaggttgaa atgtcgccct atgaccttac caaaggaaga ataacgttca gatttaaata
      241 gaaaataaaa ctgcgatgaa agtaagagca tcagtgaaaa agcgctcagc cgactgcaag
      301 attgtaaggc gtaacgggcg tctgtatgtg ataaataaga aaaatcccaa gttcaagcag
      361 agacagggat aaatttgatt aactttgcac ttcaaattta aagaggtata tggcacgtat
      421 taatggggta gacctaccaa ggaacaagca gggcgaagta gctctcacct acatctatgg
      481 cgtcggacgc agcactgctc agaaggtgct tgacaaagcc ggcgttgacc gtcaaatgaa
      541 ggtagaggaa tggaatgacg ataacatcaa tgctatccgt cagatcctct ctgagaatta
      601 caagctggag ggtgagcttc gttccgaaac gcagcttaac atcaagcgac tgatggatat
      661 tggttgctat cgcggcattc gtcaccgcat cgggctgccg gtaaggggcc agagcacgaa
      721 gaacaatgcc cgcacccgca aaggacgcaa gaagaccgtg gcaaacaaga aaaaagctac
      781 taaataagta atatatggca aagaagactg ggacattaaa gaagaaggtc gttaaggttg
      841 aacccgtggg acaggctcac gttcattcgt cattcaacaa tatcatcata agccttacaa
      901 acagcaccgg ccaggttata tcctgggcct cggcaggcaa gatgggattc aagggttcaa
      961 agaagaacac cccctatgcc gcgcagatgg cagctgaaga gtgcaccaag gtcgcttttg
     1021 aacttggcct ccgcaaggtg aaggtttacg tcaagggacc cggctcaggc cgtgagtcag
     1081 ccatccgtgc catatcaaac agcggcgtgg aagtgactga aatcgtcgac gttacaccaa
     1141 tgcctcacaa cggatgccgt ccgccttcac gcagaagggt ttaaaaggct catattaaca
     1201 tttttcaaaa cgataaacat ttcattcaat ggcaagatat acaggaccca gatcaagaat
     1261 cgccaggaaa ttcggagagc ctattttcgg ccctgacaaa tacctcgaca ggaagaattt
     1321 ccctcccggc cagcacggtc tgaccaaaaa aagaaaaaag acatcggaat acggtgtaca
     1381 gctgcgcgaa aaacagaagg tgaaatatac ctacggcctg cttgaacgcc agttccgcaa
     1441 taccttcgag aaagctgtgg ctgcctccgg agtgaccggt ttggttctcc tccagttgtt
     1501 ggaatcacgt cttgacaacg tggtttaccg cctcggcatt gctccctcaa gaccggcagc
     1561 acgccagctg gtgtctcacc gtcatatcac aatcaacggc aacgtcgtta atataccatc
     1621 aatgatgctt cgccccggcg atatcatcgg cgtcagggaa aaatcaaaat cactcgaaat
     1681 cattaccgaa tgcctcggcg caaaccgccg ttccaaactg gcctggctcg agtgggatga
     1741 cacacagatg gccggaaaat acatgagcgt ccctgaacgt accgaaatac cggaagatat
     1801 caaggaacag cttatagttg aactctattc caagtaataa agtaataata ttcatatggc
     1861 catattagca ttccagaaac ctgacaaagt aatattactg gaagcggacg accagtacgg
     1921 aaagtttgaa ttccgcccgc ttgaacccgg ctatggcatc accgtgggta atgccctcag
     1981 aagaatactc ctctcctcac tggaaggatt cgccatcact actgtcaaga tagagggcgt
     2041 agaccatgag ttctcaacca tcaccggcgt gatggaagat gtcacagaaa tcattctgaa
     2101 cctgaaacag attcgcttca ggagaatggt cgaggatatc gataccgaga agatcgtggt
     2161 taaggtctca ggacaggaag cattcaaagc cggtgacctt aataattcac tcagcagttt
     2221 cgaggtgctc aaccctgacc tggtaatctg ccgtatggaa cccgatgtca aactgcagtt
     2281 cgagatcaat atctccaagg gcagaggcta tgtgcctgct gaggaaaacc agcccgcaga
     2341 agccgaattc ggacttattg ccatcgattc aatcttcacc cctgttcgta atgtcaagta
     2401 ttcaatcgaa aactacaggg ttgaacagaa aaccgactac gaaaaactga tcatcgaaat
     2461 aaccactgat ggctcaattc acccgaagga cgccctcaag gaagcagcaa aaatcctcat
     2521 ataccatttc atgctcttct ccgaagagaa gatcaccatc gactacgaag acaagatggc
     2581 aaatgaggaa ttcgacgagg aaatactcca catgcgccag ctgctcaaaa ccaaactcgt
     2641 tgaccttgac ctctccgtca gagctctcaa ctgcctcaag gcagctgagg ttgaaacact
     2701 cggagacctg gttaaattca acaagaatga cctcctcaag ttccgtaact tcggcaagaa
     2761 gtcactcacc gagcttgacg agctccttga gacaatgagc ctctcattcg ggatggatgt
     2821 ggcaaaatac aaactcgaca aagattaata acaggtaagg atagagagaa atgcgacatt
     2881 taaatgctat aaaccacctt ggaaggaaaa gcgccgacag gaaggcaatg atggccaatc
     2941 tggcatcatc actgatcctt cacaagcgta tcagcacaac aacagccaaa gcacgcgcac
     3001 tgagaacata cgttgagcca ctcatcacca agtcaaagga tgactcaacc cactcgcgca
     3061 gggttgtatt cagttatctt gagaataaag gcgccgttgc tgaactcttc agggaagtag
     3121 ccccgaagat cgccagccgc ccgggcggat acacccgcat cctcaaaaca ggatcacgaa
     3181 taggtgacgc tgctgagatg tgcataatcg aactggtcga cttcaatgag aacatgcttg
     3241 caggcgaggg tgcaaaagcc aaggcctcac gccgccgccg cagcccgaag aaaaagaccg
     3301 ccgaaactgc tgccgtaacc gaaacaaacg caaaggctga aactcccgcc gaagaaaagg
     3361 caccggaagc cgaagaatca aaagaatagc ggcagatgct catatacgga agggatattg
     3421 tctgaatggc cgtatccctt tttttcatat attgcatatt attataacaa aacataaatt
     3481 acaatgagtc agatagttag tgttcacgcg agagaaatcc ttgattcaag gggaaatccg
     3541 accattgagg tcgaagtcat aacatccagc ggcgcatcag gcagggcggc agtaccctcc
     3601 ggagcatcaa ccggtgaaaa cgaggcactg gagctgagag acggcgataa gtcgcgttac
     3661 cttggcaagg gcgtcctcaa agcagtccat aatgtcaata atgtcatagc tgaggattta
     3721 atgggaatgg atgtcaccaa ccagatagcc attgaccaga agatgattga ccttgacggt
     3781 accccgacca agagcaccct cggagccaac gctatcctcg gcgtctcact ggcatgcgca
     3841 aaggctgcag cagcgtacca cggactgcct ctttacaggt atatcggcgg taccaacgcc
     3901 cgcgtactgc ccatcccgat gatgaacatc atcaacggcg gtgcccattc tgacgctccg
     3961 atagcattcc aggaattcat gatccgtcca aaaggggccg gcacattcgc cgaaggcctc
     4021 aggatgggag ccgaagtgtt ccattcattg aagaaagtgc tcaaggagcg cggactcagc
     4081 accgcagtgg gtgatgaggg tggctttgca cctgtgctca aaggaaccga agatgcactt
     4141 gaaagcatca ttaaggcaat caccgctgca ggatacaagc cgggcgtcga tgtgatgata
     4201 gcactcgact gtgccgcaag ctcattcttc aaggacggag tttatgacta cacaattttc
     4261 gaaggcaagg atgccaagaa gctcacccct gcacagcagg ctgattatct caccgcgctg
     4321 gttgagaagt atcctatcat tgattccatc gaggacggaa tgagtgaggg agactgggaa
     4381 ggatggaaac tgcataccca acagcttggc tcaaagatcc agatcgttgg cgatgacctc
     4441 ttcgtgacca acgttgagta tcttgagaaa ggtatcagcc ttggctgcgc aaacagtatc
     4501 ctcatcaagc tgaatcagat cggaaccctt accgagactc tcaactgcat tgagatggct
     4561 cacagggctg gctacaccac cgttacctcg caccgttcgg gcgagacaga agactctacc
     4621 atcgccgatg ttgctgttgc tacaaactcg ggtcagatca agaccggatc gctcagccgt
     4681 accgaccgta ttgccaagta caaccagttg ctccgcattg aggaggaact gggtgcaaac
     4741 gcagtttacg gctgcgagta ggcgcctggt acagatacca caatgaaaac tccgcctccg
     4801 cggagttttt atttttatat ttgtagaaat ctcttgttca atgtcattca acggtctgtc
     4861 tcagtttgta tcatttctgg agtcacgtgg tgagctggtg cgtgtcacct cccctgttaa
     4921 ccctgtgctt gagattaccg agatagctga ccggctggta aaaagcggtg gaaaggccgt
     4981 tctgtttgag aacaccggca cagccttccc tgtgctgata aatgcatttg cctctgacac
     5041 gcgcatggca gcagccatgg gacgtaattc ccttgatgat gcaggtgatg agctgtttac
     5101 cctggtcgag tcgctgaccc aggcgcgcgg cctggcgggt aagctggcgc gcctgcctgg
     5161 tatgctccgc cttctcaccc tttcgcccaa aagaaggtca ggcaggggtg agtgccagga
     5221 ggtggtggag caggtgcctg acattggcag gctgcccatc ctgaaatgct ggccccatga
     5281 cggagaaagg tttgttacac tccccctggt gcataccagg caccctgaga ccggaaaacc
     5341 caacctcggc atgtaccgca tgcaggtcat gggccgagat atgacaggaa tgcactggca
     5401 caggcacaaa accggcgcca gacactttga ggcctggaaa aagaggggag agaggatgcc
     5461 cgtcaccgta accctcggag gagacccggt ttatacctat gccgccacgg ctccgctgcc
     5521 tgaagatatc agtgagtacc tccttgcagc attcctgcgc cgtaaaagag tgtccctggt
     5581 caaatgcctt accaacgacc tgtgggtacc ggctgactca gatttcgtca tcgaaggtta
     5641 tgttgacact gccgaagcgc ctgtcaccga gggaccgttt ggtgaccaca caggctttta
     5701 ctctcttgcc gacctgtatc ctgtatttca tgtcacctgc ataacccaca ggaaaaacgc
     5761 tgtctatccc gccacaatag tgggagtgcc tccgatggag gatgcatgga tgggaaaggc
     5821 taccgagaag atattctttg cacccataaa acttgcaata gccccggaaa ttcgcgagct
     5881 ccacatgcct gtttcgggcg tggcccacaa cctggtgata gtgagcattg agaagaccta
     5941 tccgggtcag ggaatgaagg tgctgagtgc actcttcggc gcagggcaga tgatgctcag
     6001 caagtatata atagtagtca gcggcaacgt tcatgtgacc gattaccgcg ccgtggctga
     6061 ggaggttttt gccaatgtaa ggtttgattc tgaccttctg cttaccaggg gccctcttga
     6121 cgtacttgat cactcctcag acaggttcag catgggaggc aagctcggca tagacgcaac
     6181 cgtgaagctc tcggaggaac gaaccgatga atatcattcg gttccgctgc tccttctcac
     6241 cggcgagcag ttcaatgaaa aggtcatcag cgggatgaag acactggctg attatgccct
     6301 gccggtggca atacttacta taaccaaacc ggaggagggg cttgacatgc atcgcctggc
     6361 agcctctctt cccgcatcac ttaccacacc gggtaccgtc atcattgcag tggatgaggg
     6421 agctgatgct gatgaccccg ccatgcttac atggctggtt accggcaaca gcgatccggc
     6481 ccgtgatctc taccatctcg agggcggcgc catctttatt gatgccacct caaaaacccg
     6541 gtccctgcct gctttcccca gggattggcc aaacgtggtc tgttcagacc cggagacaat
     6601 tgaacttgta aacaccaggt ggcctgaata cggactcggt cctttcatag aatcaccgtc
     6661 cctcagactc cttcccctgt taaagcctgg cgaagcttcc gtggagacgg ttaattcaac
     6721 tgccgggtga caggcttacc gtcagcggcc ctgttgactg cctttgtgca gggactgaga
     6781 gacagcccgg ctctcaatta cctcattgtg atcttcatca tcgcggcact ggtctacctg
     6841 atcgtactcc agttctgggt gctgctccgt accaaaaaac acctgcgtga aatacagggc
     6901 gcatacagcg agatagaggt gcagagatcg gaactgcaac tgagaaacaa ggacctgact
     6961 gacagcctga actatgcccg caggattcag gctgcgcttc ttcctgccga acatcacata
     7021 cggaggattt ttcccgatca tttcatctac taccgcccga agcatatcgt cagcggcgac
     7081 ttctactggt tcagtgagcg tgacgaaaag tacttcattg cggcggctga ctgcaccgga
     7141 cacggggtgc cgggagccct tatgtcaatg atcgggctgg aactcattca gaagatcatc
     7201 aatgagatga aggtcgatga cactgaccag gtgctgatca ccatgaaccg cgagctcgaa
     7261 tcagccttct tcaaggagga aagcggcaaa gccctgatta aggacggaat tgagatgggt
     7321 atctgcgtta ttgacagaaa cacgaaagtg atggagttct cgggcgcatt tctgccggtg
     7381 tatatagtcc gtgatgacaa gctgatagag atcaagggcg acaagaagaa tgtagtgcag
     7441 tcatttgcca tggtatcatt caaccggagt actttcacgc tgcaggaggg agatatcctc
     7501 tatctcttct ccgacgggta tgctgatcag ttcggaggac cggaaaacaa aaagtttatg
     7561 tatcgccgcc tgaggcatat cctgctgacc ataagcaaat atccgctggc cgaccagcag
     7621 cggattctgg atgaaactat cgggtcatgg atggaagggc atgaccagat agatgacatg
     7681 atgatcctgg gggttaaacc tttctgaggt cagataagag ctgtaatctt ctctatgagc
     7741 acctcgggct ctataggttt gcttatgtaa tcgtccatac ctacagccag gcactgttcc
     7801 ctgtcgccca gcatcgcgtt ggcggtgatg gcaatgatag gtacgtgtcc gctggagctc
     7861 atctccagcc cgcggatctt ctctgccgcc accagtccgt tcataaccgg catctgcaca
     7921 tccataagta tcaggtcata gcgagatgtg ccaaacatgt caagagcctc cttgccgtta
     7981 cgggcaacct cgagcctctt tacaagcggt tcaagcatca gctgggtgat tcgctggttg
     8041 atgggattat cttccaccag cagtatgtta agctctttca gttccttatg actcttctcc
     8101 gtctccatct ccctgaccag ggtgcttgct gctgccgggc ggatcacttt gtctgccctc
     8161 ctgaggaaga tatagaagac gaagtatgaa ccggttccgg aagtggactc ctgccagttg
     8221 cccccgcaat cgcgtatcag tcttcctgcc agagggtaag gctggcgtcc ttcaccgatg
     8281 aagactgcct ggttgttcga tgacagggtg aattcaacct tgtgtgctcc ctccctttca
     8341 ccggcttccc tggcctgtat cttgatggtc acctttcctg ccggccctga gatggcagcg
     8401 ctgagaagat cgatgagtat ctgcttgatg atgatcgggt cacccctgta ttccctttcc
     8461 ccctcgccgt ggtcatcata gaccaccacg ctctggtcat gtctcctgct gagcttcatc
     8521 agaccgatgg tatcacggat gatgggcccc agcctgaaac ggatctcacc ccgcctctca
     8581 aaggtgatat tgccggctgt ttccatggta agttcattga ccacctcaac cattgatccg
     8641 gtggaagcaa tgaaggtctc cacaagctcc tgctgcttgg gtgatgaggt ggtctcactc
     8701 agcatgttgc ccagcaggac gaggttgttg aggggctccc ttatcttgtg cgaaaaggag
     8761 ttgatcacct cctcgttctt ttcggcaatt gaagccagct ccctgagaat ttccctgttg
     8821 tctgcaatga gcttgcgaag ttctgctatc gccagtatca gcgggaccag ggcaagcaca
     8881 cctgccgcta tcggcatgta caccagcccg gcaagggcgg tgacaagggc tatgaccacc
     8941 aggagaagcg ctacggcaac cggcagcagt atgcggtccc tcgtccaccg ggtcatcagg
     9001 tctgagtgcg gtatggcaga tggtcttgat ctgttcggca tatcgtgaac gcagtagatt
     9061 aacgtcactc aaagatacat caatttcaat aaagataaag agagccatgt tctcagtgca
     9121 gagaaaagat tatttttgcc ttacggtata atttttgagg tgaaatctga aaaagagtgt
     9181 gatgaaaaag tgcttattga cactgatcgc agtcctggtc gccaccggct tcaccagggc
     9241 acagcagggg gatactcttg ttcagtatac tcctgacttc aagttcaggg acggtatcta
     9301 cctgaacttt gacatggtta agacaaacag ccccatccca aaggctaagc tcttcacatc
     9361 ggttgattac aacgacaagg atttctttga ccaggtgatc tcctccgaaa agatctattt
     9421 ctatgataac atgggcgtca ggcaggagat agagaaggat gcaatatggg gattcgcccg
     9481 gaacggcgtt ctatatgcaa aggtgcagaa caacttcaac cgcatcacat tcttcgggtc
     9541 aataatccat ttcgtggccg atgtcacaac ccagacaggt tacaacccct acgggtacta
     9601 tgatccctat tactcaccct acaggtcatt ctactcgcca tattattaca gcccatactc
     9661 ctcgcgctac tatgacccgt actactacgg cggatcaccc tacagccgaa acacatcacg
     9721 tactgacctt gaaatgttca tgatcgattt cgagacagga aagacatacg agtatgacct
     9781 cgagaacctg gaagcactcc ttatgcggga cccggagctg tacgaggagt ttgtatcaat
     9841 gcgcaacaaa aaccagaaaa aaatgatgtt tgtatacctg aggaagtata acgaaagaca
     9901 cccgattctc cttccttcag gcaaataaag gtttaatgaa aaaatttccg ctcctcgccc
     9961 tggctctcct gctggcctca gccatatccg cgcaacagag accataccct acatttgatg
    10021 acgctgcaaa attcaagaaa tcagtaacat gcgttgtcag ggaagatgat cccttctcat
    10081 tttacaatgc agagataaag gctgcagttg acaaatactg gaaggtaacc cctgtgaaat
    10141 atattaccac ggaggagttc aacgtcatgc gcaatgaccc tgcttactct ttcctggttc
    10201 ttactatcac caacttcagc aatgacaagt caggctcaac ctatgacttc cttaacctgc
    10261 tgctgggcgc cgacgttgac gagcttgacc agctgccgga attctgcgcc attccgctct
    10321 gcttctcggg agccccggag gaggaataca gttacaagct cggccttatc atccggttca
    10381 tggaatacca cgccgaactg gttatgaaaa accccacgct ctcagcattg cggtacctca
    10441 aatattacaa caaaaacgtc cctgaaataa aggacaagac catcctggtg cggcaggccg
    10501 acctggcccc ggaggtggac tctccggaaa ggattgctgc atattatccc tacaaggtca
    10561 ggatagtgga tgaagaggaa atcattcgcg ccattgatga aaagcagaag gatgtcctga
    10621 ttgtccacaa ggtgggcccc gaaggcgtca agaggacagg tacctgcatg aaaaccctca
    10681 tcgggacaga tgatgcgata atgtattact atgacagtca tatggttgac agtaaaaatg
    10741 ccagcggcct gctgattgcc gacctgaaga ggctcgcccg cttctgagga tgcagcattt
    10801 ttaaccggaa accgtcttta agaaaaaagc aatggaagag accaaaacct acacacagtc
    10861 agagcgcgac tgggcgatgt tctgtcacct gtcaaccttt gccggttact tcttcccctt
    10921 cggggggatt atcgggccgc tcatctgctg ggtatcaagg aaggaccagt ccgaatgggt
    10981 tgacacgaac gggaagtcag cacttaactt ccagatttcc atgttgcttt ataccatttt
    11041 acttattccg ctctgcttta taatcgtcgg gattccgttg ttggttgccc tcggcatcct
    11101 tgaggttgtt tgcgtcatcg tggcaagcat cagggccgca aagggcgagg cattcaaata
    11161 tcctatatca atccccttca tccaatagac aaattatcat caatatacgg taacgacagg
    11221 tctgagacct gtcgctacgt gataatgata cacgatttgt atacggggga atatgatccc
    11281 tgccatgatg atgatgatgc acaatccgta tatggggtaa catgggccta tccccctttt
    11341 ttttcctatt tttgccggga aactatttcc cgtcatggct cagaaaactt ccatccccaa
    11401 gggcacacgc gacttctctc cggcagagat gctgcgccgc aaccatatct tcggaataat
    11461 caggaagatc ttttcgctct acggctacct gccgatagaa acccctgcga tggaaaacct
    11521 ctcaaccctt ctgggcaaat acggggagga gggcgacagg ctcatcttca ggatactcaa
    11581 ttcgggaaac ttcctggctg gcgtgtcgcc atcggtgctc tcgtctgcag acacatcctc
    11641 tgtgatccgc cagatcagtg aaaagggact gaggtatgac ctcactgttc cattcgcccg
    11701 ctttgtggtg cagcatcgtg acgagatcac attccctttc cgcaggtacc agattcagcc
    11761 cgtctggagg gcagacaagc cacagaaggg gaggtacagg gagttcttcc agtgcgatgt
    11821 ggatgtgatc ggcagcgact cactgctcaa tgaggctgag ctcgtacgga taattgaccg
    11881 catcttcagt gagctcggca taaacatcat tatcaagata aacaaccgca agatcctggc
    11941 aggtattgcc gaaattgccg gggcagggga gaggatcacc gatatcactg tggctattga
    12001 caagattgaa aaaataggca gggaaggagt aaggcaggaa ctggcccaga gaggcatcgc
    12061 acccggggtg attgcaaggc tcgaaccggt catcgacctg agcggctcaa cggaacagaa
    12121 actcaccgag ctgaggaatg taatggccgg atcagtttca ggagtgaaag ggatcgggga
    12181 gatggaggag cttacagggt atcttgccat tgaaccggtg aaggcacctg tggagcttga
    12241 tctctcgctc gcacgcggac ttaactatta taccggcaca ataattgagg tcaaggcggc
    12301 agaccttccc ttcggcagta tctgcggcgg gggccggtac gatgacctca caggcatctt
    12361 cggcatgcct ggcgtatcag gtgtcggtgt ctcattcggg gctgacagga tttatgacgt
    12421 gatgatgcag ctcagcctgt tccctgagca gaccggctca tccacagagg tgctcgtaat
    12481 caatttcggc agcggggaac tgcctgcact gatgaaagtg gccggtgaac tgagggaggc
    12541 aggcatcgcc acagagctgt accctgaccc ggcgaagctt aagaagcagt ttgcatatgc
    12601 tgattcaagc atgattccgc tggtggtgat agccggcagc aatgagatag ccgccgggca
    12661 ggccactgtc aaaaacatga aaacaggaga acagacagct gtcccgctcc ctgaactccc
    12721 gttattcata cgccgggtaa tgacctcctg aacaatcact cggtcaacgg ggcaggtcct
    12781 gactgaccgc tgctcctcct cataccctgt ccctgctgtg ccggaccctg ccttctctga
    12841 ttcagctcac gcagcaactg ctgcttgtac tggttctcaa cctggtacag cctgataacc
    12901 ttggctggag gaagtacctt cttcaggttc tcattgaatg ataccaccat tgccgcctca
    12961 tcggcaaatg accgggtcag attatccgct gtggatgcaa cctcgtcttc agtcatgttg
    13021 gcctggttct gagccgcata gcgcataagc aacagacggt cggcctgcag cttgctcttc
    13081 cttgccgaat aatcattgta aaccggccag aatttttccg cctcggcagg agtaaggtcc
    13141 aggttctttg tgaagaaggc aatcttatag gcggtgagct tctcaccgtc accctgcatt
    13201 gcaccctggc ccataccctg gccctgtgcc ctgagggtca tggctgtgac tcccgtcagc
    13261 aacactgcag caatggttgt aatgattctc ttcatagcat tataataata gttcatatat
    13321 gtcatcaagg ttgatatctt cagtcatcag gtaatcaatg atagtctctg acgagattgt
    13381 ctccccggtt atctccggga tgaccgggtc agtcatgttc aactcatctt caagcatgta
    13441 tgtgcctatc tcttcaacgg cgaggtcagc atagaggctg ttgccggaat cagacaccct
    13501 gtccggccgg tcaacattga tcagcctgac cagtgcggat gcaagaatgg cgaaacccag
    13561 tatggcagca gccagggcca ggaagggttt cagtcttaca atcatgccag gcttgcggag
    13621 cttttcctcc cttatcgcgg ctttcccgtc cccctccgtt atcgtggccc agtcctcacc
    13681 tttccctgcc accaccttaa tcacggccca gtccttacct ttccctgcct gctcatttat
    13741 tgcggccatg gtgcggtcag ccagtgtgtc gaagtaatct tccggcacac tgaatggatt
    13801 ttcttttcct ttttcttcgc tgagtttcat gatatctctt tgacaccctg accgggaata
    13861 ggtttaatcc cggtcaagaa attgttcaat cttttttact gcgtggtgat atgaagcctt
    13921 aagggccccc accgaggttc cggttactgc gctcatatcc tcataggtca tctcttcgaa
    13981 gtacctcatg ttgaatactg cgcgctgctt ctccgggagt ttcaggatgg cattctgcag
    14041 tctgaccgtc gcggcatcgc cgtcaaacca cgtacttcct tcagccgagg aagcaaggat
    14101 ctcctctaca tcatcaagtg ccacgctgtt ctcacgtccc ctccgcctta tgatattaag
    14161 agcctcattg gtggcaattg cccatagcca tgtgagcagc gagctctcag acctgaatga
    14221 tccgatgttt ttccatgcat tcaggaaagt attctgcagc gcgtcatcag cgtcatcatg
    14281 cgtgatcaca agccggcgga tgtgccagta gagcttccgc ccgtaaagat caatgatcat
    14341 ccggaatgcc ttttcggtat ccgctgccag agctttcctg atatcatctt cagtactttg
    14401 cgacattaac cttccttggc ggttttagtc agtcagacgc ccggaggcgc caatggttta
    14461 atctacactt caaacttagc ctctttttgg taactttgca gcaacagaat catgagacat
    14521 ggcattgaaa tgcggaatta tcggaatcac cagcgtaggg aagaccacta tcttcaactg
    14581 tatatccaac acccgcggtg agactaacac atacggcacc ggagtgaagg ccaacctcgg
    14641 tgtaatccag gtgcccgaca aaaggcttta tgagcttgaa aagttccagc cgaccgaacg
    14701 gatagtgcat acaacggttg atatcgttga catcccgggg ttggtcaggg ggcagggcga
    14761 cagctcaggg gcacgcctgc tcggcgagat acggaacacc gatgccctga ttcatgttct
    14821 ccgatgcttt gatgatgaca atctcccgca tatagacgga tctgtcaacc cggtccgtga
    14881 cctcgaaaac gttgagttcg aattgcaggt caaggatgtg gagtcagtgg tgaaaaagat
    14941 tcagcggctg gaacgggctg caaaaacagg cgacaaggag gcaaaacacg gtgttgagat
    15001 tctaaatctt tacagggaac atctcgaggg ttttcagaac gcttcttccc ttgagctgaa
    15061 acctgaagaa aaaaaggttg tggatgatat tttcctgctg accatgaagc cggtactcta
    15121 tgtatgcaat gttgacgagg cttcggcagt tgacggaaac aggtacgttg aggatgtaag
    15181 aagcttcctt gccccgagga gcactgagcc gctggtgatt gccggggcgc ttgaggcgga
    15241 gatagccaac ctcaccgaag aggcagaccg cctggagttt ctgggagcct cgggactgac
    15301 ggagccgggt gtgaacaaac tcgtcagggc agcctacagg atgcttaacc tgcaaacctt
    15361 ctttaccgtg gggccaaagg agataagggc gtggaccatc accgccggca tgaccgcacc
    15421 cctggctgcc ggcgtcatcc actccgacct cgagagagga ttcatcaggg cggaggtgat
    15481 gaagtatgat gatttcatca ggctcggctc cgagcacgcg tgccgcgaag ccggaaaact
    15541 gatggtggag ggcaaggcct acgtggtggg cgatggtgac atactccaca tccggtttaa
    15601 cgtataacaa ttaaattgaa gctccgaagt gtcataccgg tactcctgct gctggtttca
    15661 atgcaggcgg tctgcgggca ggagagcccg gccggggatg atatccggaa tgccgtcagc
    15721 aggtacggtc aggctgaagt gatgatcagc tatcccggtt atgatgcaat gaccaggctg
    15781 gctgcacgtt tctcagtctc atcctgcgac ggtctgactg ccctgctcag cctctcttcg
    15841 cgtgacgtcg cggagttcat cgcggaggcc ataccctaca ggctgattgt gccggaggag
    15901 cggaagggtt tctataccgc ctcatcggta gccgaggcaa tgctctggca gtcatatccc
    15961 acctggaagc attatgacac aataatgcat aagattgccg gccactggcc tgacctctgc
    16021 cgccttgaca ccataggctt cagtgtccag gggagggctg tgctggcatt aaagatctct
    16081 gataacccgg aggaagatga gcccgagccg gaggtgatga tcagctcctc aatccatggt
    16141 gatgagacgg caggttttgt ccttctgatg cgcctcgccg aataccttgc ttccggcggc
    16201 ggcggcgcgc ccggatcagg gttggcttcg gaactggtgt cagaacttga aatctggatc
    16261 aacccccttg ccaaccctga cggcatgtac cgcaatggcg acaccctgac ctaccccgta
    16321 cgcgccaaca gcaacggcta tgacctcaac cgcaacttcc ccgacccaga ggtgggcacg
    16381 ccgccgccgc tgcagaaaga gacacttgac atgatcaggt tcatggaaga gagaaggttc
    16441 actctctccg caaacctgca cagcggtgcc gaggtcgtga actacccgtg ggacaaatgg
    16501 acgagagacc atgctgacga tgactggttc aatgcaatca gccggcgtta cgctgatacc
    16561 gttcacctcc attctgaatc cggatatatg accttcctcg ataacggcgt taccaaaggt
    16621 tggaagtggt acctggttag gggtggaaga caggattatg tcacttgggg ccttggcgga
    16681 cgggaggtga ccatagaact cgatgatacc aagatgaccc ccggctcaaa cctggaactg
    16741 atgtgggaat ggaaccaccg ttcactgctt cgctatatcg ccggggcgat gacaggagta
    16801 agaggaacgg tcaccgacgc cgaaacaggc gatccgctca gtgcgaagat tttcattgcc
    16861 ggacatgatc gcgattcatc acatgtctgg tcagacacca ccaccggatt ttatacccgc
    16921 ctccttccgc ccggcacctg gaacctcacc ttctcatgcc ctggatataa accatacacg
    16981 ctcaccgcag acctcacctc ctccgttccc cttcaggtca gcaatatcca acttgaaaag
    17041 ggcaaccatg actaccctga tccgccggaa tcaggactgc tggtgttccc caacccgtca
    17101 gacggaaact tcagcatcat gcctcccgtg gccgttacag gcgaggtgac cattacagtc
    17161 acatcaactt ccggcgccct tatgaaacac tacacgacaa ccgcccagcc aggcatcccg
    17221 gttgaatgca aattcaccgg cctgccggcg ggaatcttca ttatttctgt aagcagggag
    17281 cccaatggtc ccatggtaag gggcaaagca gtcatcacac ggttgataaa ccggtgaccg
    17341 atggtgatat cttttccgga ccgcgttgca gataaaacca aaactgatat ttttggaact
    17401 tatttccaag gagggctttt gaagatgaaa cgggtaatta cagtattata tctgcttata
    17461 ttaactttca gtgttacagc gcagtcggct tacattccac ctgacaaacc gaagcttgta
    17521 ataggcataa ttgtggaaca gctccgttat gaccagcttg aacgcatatg ggagattctt
    17581 cctgacaacg gtctgaagag gatggtcagt gaaggcacct attaccgcaa cgcatcaatt
    17641 gactacctct ccacccaggc ggctcccggg tttgcaacca tatccacggg ggcatctcct
    17701 tcagctcacg gcatcacctc tgattcatgg tttcatccgt tcaatgatga gatgatatac
    17761 tgtgttcagg atgcaactgc cagtcctgtc ggaggcagtt tcgagacagg cctcttctca
    17821 ccagttaacc tgctctcgtc gacatttgca gatgagctgc agatggcttc atgcggagga
    17881 tcaaaggttt acgggatagg catgaaggag atggctgcca tcatcacagc cggccacgcc
    17941 gctgacggcg ccttctggtt cgatgaccgt accggcacct ggatgtcaag tacctatttt
    18001 atcaaatccc tccccccatg gctcatggat ctcaacgcca tgatgatgcc gcaacagctc
    18061 ctcaaccagc aatggatgcc cctgcatgat ccggcaactt acccgggatg ccagcctgac
    18121 tcaagcagcc tcgagagggg attcaacggc cagacatggt tcccgtatga cctgaaggct
    18181 ctctcaacca aaggcaaact gctcaatgtc acaagggatt tctccgttct ccgcgatact
    18241 ccgtttgctg acgagtttac taccgaactg gctctcagat tgattgacaa tgagcagttg
    18301 ggccaggatg atatcactga ttatcttgca attacatatt cctccaccga ctatgtcggg
    18361 catcgtttcg gaccttcctc cgttgaggca tcggatgcac ttgtgagact tgacaggaac
    18421 atagccaggc ttgttgagaa gattgacaag tcgctcggca agaagaatgt ccttgtttac
    18481 ctggtgtcgg ctcacggagt ctcagagatt ccggcggtgc tggaacagag cagggtgcct
    18541 gctggttatt tcaggctgaa ccagtcactt cagctgctcc gcagttatct gaatgccgtt
    18601 tacggccagg gcgactgggt gcggggcttc ttcgacagcc agatatatct taaccgcggt
    18661 ctcattgagg gtgctaagat caaccttgaa gagatgcaga agagggtggc acggttcatg
    18721 gtgcagttct ccgggatatc agccgcagtg cccacatcag cctttgagat gagtgatttt
    18781 tcgggagggc tgttgcgcaa gatgagcaac aactacagcc agcaacgttc cggtgatgtg
    18841 atgatcgccc ttaacccggg ctgggttgag aagactgaca atgttacagg ccataattcg
    18901 ccatgggagt atgacagtca cgtgccgatg atctggtttg gctggacagc cagcagggta
    18961 tcagtcaccc gcagtgtcag cacccgggat attgcaacca ccctctccgt actgtgcaag
    19021 gtgccgctac ccaacgcgtc gagcggtgat ccgttgcacg aactgttcag gtaaattctg
    19081 tccgggctgc ggcccggaca acgtcagatg atgacagcac cacggttgaa gctgtccctg
    19141 acgaggcagg caccttttat cagcgccggg ttctgctcgc agccgttacc gatgacaacc
    19201 atgtcagctc cggcatcaaa agccatctcc acctttacag cggagtcaat ccctccgccg
    19261 gtaataaggg gtatcgtcag ttcatcacgg acagccttta tcattgatgg atcaacaggc
    19321 ctggcagcgc cgctgccccc ctccaggtaa atggccttca tgccaagcat ctctcctgcg
    19381 agtgccgtgg ccacggcaat atcattcttt cctgacggga tgggtgatgt ctggctcatg
    19441 tactccactg aggtgcctcc cccgcagtca atgagcatgt aacccaccgg gattactctc
    19501 tccctcatgt ttttaagaac aggcgctgcg attacatggc tgccaataag cagttcgggg
    19561 ttgcgcccgc tgatgagtga cagcatgaaa actgcatcag ccccggcagt gagctgtgtg
    19621 agatttcccg gaaagagcac cacagggatg gatgagagct gtttcagcct ctttattaca
    19681 tcagccacgg gcacacttgt cagactgccg cctgccagga agatatccac acctgcctca
    19741 tcagctaacc gtgcagcatt gacaagcgtc tcttcaccgg cgcggtcagg atcaatcagc
    19801 tgggcaacaa gctttctttt cttccagggt atctcaacta tcatgtttct tcttcgtcca
    19861 gacaatagtg taattgtcgt attttgagta atatagcgtg aaccggtcac tgattctctc
    19921 gttctccaca ctgcccctga tctctccgga ctccttgacc acaaaaggtt caatggtgat
    19981 atcattgacg atgtttatcc cctccttgtc acagatcttg tagatggctt ccttggcaca
    20041 ccagtgaata tacaggtgat aggtacggtc agcccacctg cgggtgactt tctccctttt
    20101 gttcataaac ttcgaggcaa aagcattgat gttcacccgc atgtactcaa ggtcaatgcc
    20161 gactctctcg gtcttgctta acagtattgc tgtaagcttg ttggaatggg tgatgctgat
    20221 gaagtgcgag ccgtctttta tgaatggctt attgttcttg ttatagacta tcctggcctc
    20281 actgccgagc atctccgcca gcagggccct tacactcagg aactctatct tcctcgagtt
    20341 gctccggaag aactcatatc tcttccggtc ctcggggctg aactccacca tcgaaaggag
    20401 agtatcgata tcctcttcga tcttccagac gccgataacc gtctccttat tcagatagtg
    20461 cttggtgatg cagcccatgt tctaccgcca gttcagtgtc tcgataaggt gaataatgtc
    20521 ttccctgatg aatgaaatca ccggcatgag tgagtcttca ttgggctggg ctgtgaaata
    20581 aagtgatccc ctcaggtaat ggcgggtgct gtcagtgacg aagaactgca ccgccgaggc
    20641 ggtgttcccc ttcaggtcat agagcatccc gtaaacccct gtcgactcat ctgcccagag
    20701 cttctcgtcg atagcctcag ccctgatggt gtgtttgtat gccatttccc tcgacatctc
    20761 ggtcagcgat gcgaggttat catgtatatc gaaatatgtg aggtgtatct tcgccttcag
    20821 cgaagggaat tcgatgtcca tccagcaggt gtcagtggct gctgccggat gcaggttgac
    20881 aacggcgtag tgagggtact cgatggagta ggaacagggc gagtcaaaat gaatatagct
    20941 tttctgcggc aggtcaatcc tgaagtatcc tttcggtttt ggcaccgtcg gatggtcaca
    21001 gccagcaagt atcagtgcag aggccaccag tgcggcagct atagtttttc tcatatgcct
    21061 tcccctttct tttcagcgga gatctccctg atccgccgct tctcaatgct ttcaattgtg
    21121 aacctgaagc ctcccgcagt tatctccctt cccttgaccg ggatttcccc gagcagctcc
    21181 agcagcaatc cggcaagggt ctcagagtct cccctcactt ccccgaacgg gtcatcttcc
    21241 agccctgtaa tgcggtaaaa atcattcagc agtatcttac cgtcgaatac ccatttgcct
    21301 tcacctgtct gcttgtaaag cggcacctct tcgtctgttt catcggtgat ctcacccact
    21361 atctcctcca gcacgtcctc aagggtcacc agcccgcttg ttcctccgta ttcgtctacc
    21421 actatggcca tgtgtatctt ctttgcctgg aattccttga gcagctcgct tatctttttc
    21481 gtttccggta ccacgtaggc agggcggatg agcgactgcc acctgaagtt gtcaggtttg
    21541 ccgagatgtg gcagcaggtc cttgacaaac aggatacccc tgacgtggtc aaaggtttcc
    21601 gaatagaccg ggatgcggga gaagcctgat tcgatcactt cgtgtatgat ggcatggaat
    21661 cccttgctta tgtcgagggc ggtgacgtcc atccggggac acatgatggc gcttgcgctg
    21721 gtgttgccga agcgcacaac ccccttgagc aggtccttgt cgtccttgat ctcggatgat
    21781 gtcaggtcaa gagccactga caggtcgtca atgctgaggc cggcatccct tgacttctgc
    21841 ctgcgcccga caaaaaacga cgagtaagcg aggagggagg ccagaggcct gaacagcttt
    21901 gcggtgaagg tgagcgccgg cgccatcatc agtgcgaatt tcagctggct acgcgaggca
    21961 ataaccttgg gaattatttc gcagaagaga agcaggataa aggtgatgac caccacctca
    22021 atgatgaagg aaaggaccgg attattgctc agatcaaaca gcctcgagct gatgaatgcc
    22081 gccaggatta tgattgcaat gttgatgaca ttgttggtca caaggatggt gctcagcagc
    22141 ctgtcaggct ttgcaaggag cttcatggca gctgattgtc tcctgcccct gcggtgctgg
    22201 agctcttcca tgtcaccggg ggtaagagag aagtatgcca cctcagaacc ggagatgaag
    22261 gcagatgcta taatcagaag aatcaggacg ataatttccg tgcccagggc cacgggtgat
    22321 cctgtaactg cggccagagc cattattccg gttaatgaac ctgcgggtag cgtctccaaa
    22381 atcatccggg ttggttacct agaagggcag gtcatctgca gtgtcatggg aggagaggtc
    22441 ttcaaccggt ccttctcctg ctgaaggaga tgatggctga gaaggggcag aatacccgtg
    22501 gttgctgccc tggttgttgt tctggtatcc ctggttgccc tggtaaccct ggcccggctg
    22561 gcctgcgttg tcaggcttac ggcccagaag ctgtatcacg tcggcttcaa cctccacgat
    22621 gtatctcttg ttaccgtcac ggtcatcgta tgacctgtag cgaagctttc cctccacgag
    22681 tatctgtgat cccttgcgta tgtagttttc agacagttcg gcaagccttc tccatgccac
    22741 gatgttgtgc cattcggtct gtgtcacctt ttcacctccc ctgggtgtgt aggtttcgct
    22801 tgttgcgagt gaaaatgatg ctttaatgtt gttctcgaaa cgccggatct caggatcctt
    22861 gccgacgtta cccaccagta ttaccttatt gaatgacata gctttagtat tattttactt
    22921 atctgaaagg atgtaaagtt acgaaaaata ttgctgtgaa gtgatcgggg cgttatctcc
    22981 cttgagggag ccgccttccg ggcctgcccg aagggtctgt ttttaccccg tatattttat
    23041 aggtatcaaa aaatcagcaa tttcggactg aaaaaaaagc tatgtcaact gttattgata
    23101 aataatattt tatatttgca cattgaaaaa acagaacatc aaaataattt gttgaattaa
    23161 aaattcttgc aacatgacaa aagcagacat tgttaacgaa attgccaaga acactggaat
    23221 tgagaaagtg accgttcaga aaaccgttga ggctttcatg gaatcagtaa aagactcgat
    23281 ggttgccggc aataatgttt atctcagggg atttggcagc ttcatcgtga agaagcgcgc
    23341 aaagaaaaca gccaggaaca tatcaaagaa cacaaccatt atcattcctg ctcacaatat
    23401 cccggcattc aaacctgcca agactttcat caacaaggta aaatcacacg ttaagaaata
    23461 acaatatgcc aagcggtaaa aagagaaaaa ggcataagat ggcaacccac aagcgtaaga
    23521 agcgcttgcg gaaagacagg cataagaaga agaagtagca taaatgcata gtctgtagct
    23581 cttatatcta acctaaagat cctctgatct ttaggttagt tgcattataa aaaggttata
    23641 ggctggcaac aaattccgga atgtgaataa ggatttaatt atcagcgtaa atgatgctga
    23701 gatatccatt gcactactgg aggataagca gcttgtcgag ttaaacaagg aaaagcggaa
    23761 tgtaaaattc tcagtcggcg acatttacct gggtaaggta aagaagatta tgccggggct
    23821 taatgcagcg ttcattaacg ttgggttcga aaaagatgct ttcctgcatt atcttgacct
    23881 gggagcccag ttcaggaccc tccaccgtta ctatcagctg gccacacaga acaaggccag
    23941 aatgacaaca gtgtcaaagt tcaagcctga ggctgacatt gacaaggacg ggaagatagc
    24001 tgatgtactg acatcaggac agacagttat cgtgcagata accaaggaac ccatctccac
    24061 aaaggggccc aggctggcct cagaggtgag catagcaggg cgaaacctgg tcctcatgcc
    24121 attctcagac aaggtctcag tctcttcgaa gatagagaac gcagatgaac gtaatcgtct
    24181 caagctcctg atacagagca ttaaaccaaa aaattacggc gtcattgtgc gcactgtcgc
    24241 agaaggaaaa aaggtggcag ccctcgacgc tgaactgaag gaacttatca accgatggga
    24301 aacagctctc tccggagtgc accgcgacgt caagtcgccg cgactgctcc tgggcgagat
    24361 gaaccgcacg acaac
//
LOCUS       DAOPXU010000037        23129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_2228_length_23129_cov_12.877308, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000037 DAOPXU010000000
VERSION     DAOPXU010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23129
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2228_length_23129_cov_12.877308"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..607
                     /locus_tag="PKK03_05475"
     CDS             17..607
                     /locus_tag="PKK03_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNX83882.1"
                     /translation="MVKKKSHEERHDEPMPEKKTADNQINTESDASGQHEPEGEPEAT
                     VKSQAEEELLGKLTEMQDRYLRLSAEFDNYRKRTLKEKIELSQSGGESVIKKLLPVID
                     DFDRAMNSMRSTDDCNAVKTGLELIYSKINDFLKQNGVKEIEAMKEPFNSDFHEAVTT
                     VPVEDDKLKGLIIDVTQKGYTLNDKVIRFPKVVVGE"
     gene            630..1784
                     /gene="dnaJ"
                     /locus_tag="PKK03_05480"
     CDS             630..1784
                     /gene="dnaJ"
                     /locus_tag="PKK03_05480"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNX83883.1"
                     /translation="MSKRDYYEVLGVSKSASKDEIKKAYRKQALKYHPDKNPGNKEAE
                     EKFKEAAEAYEVLSNDEKRARYDQFGHAGMNGGGFGGGFGGGGMTMEDIFSSFGDIFG
                     DAFGGAFGSRRGGSRSRSVNKGSNLRLKVKLTLEEFAHGAEKRVKVNKYVTCNTCHGS
                     GAASDSDVTTCTACKGTGHTTRVMNTILGQMQTTAPCNVCGGEGKVITKKCPKCYGEG
                     ITQAEEVIPLSIPAGLAPGMQLSVTGKGNAARRGGINGDLIVVIDEEPHDELIREGND
                     LIYNLFISIPDAILGANVEVPTIDGKVRIKIDPGTQGGKILRLRGKGLPDVNGYGKGD
                     LLVNVNVWIPKNLSREEQKAFEQFKNSSTFVPKPDADDKGFFDRVKNYFS"
     gene            1899..2825
                     /locus_tag="PKK03_05485"
     CDS             1899..2825
                     /locus_tag="PKK03_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX83884.1"
                     /translation="MILFEAKEVTKRFATQTALDRVSVSVPEQSIYGLLGPNGAGKTT
                     LIRIINQITAPDSGAIFLNGRPINPDDVNSIGYLPEERGLYRKMKVGEQALYLAQLKG
                     LSHAEAMRRLKYWFKKLAIIDWWDKKVEELSKGMQQKVQFVTTVLHEPKLLIFDEPFT
                     GFDPINVKQIKDEILFLRERGATIIFSTHNMSSVEELCDNITLINKAQTILEGSVEKI
                     RHDWAGNEFEVVFGGEVNPSSNGNFTLLSSVFDEGKSRVVIKASEPVDNNAILRAMTA
                     AGQVLSFNPALPSMNEIFIRVVETKNKEAVKN"
     gene            2829..4187
                     /locus_tag="PKK03_05490"
     CDS             2829..4187
                     /locus_tag="PKK03_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX83885.1"
                     /translation="MNKIALIIRREYITRVRKKSFLIMTFLTPFLFALIFIIPALVMT
                     NEDKDFKKIAVIEDGSDLFTNVIPDTKDTDFEYLEGVKLNDIKNNFAELGYYGVLYIS
                     PDLVANPDAIQLISRKQPPIGLLDHISGSLEKEIEKQKLLAYNIENLDEILRTINTSV
                     RVQTIKMDETGSARETSTGISMALAYIGGFLMYMLVFMFGSMVMRGVIEEKTNRIVEV
                     IVSSVKPVQLMLGKIIGVALVGLTQFALWIVLTLALVMVVKTNLLPEGAMEQLQQLPQ
                     SFAEADQATAAGSAGTTVTAEQLTEFQQIFAGALSMPWGLIIFSFVFYFLTGYLLYAS
                     LFAAIGSAVDNETETQQFMLPVTIPIILGLVVAMGTMQNPESSLSFWFSIIPLTSPIV
                     MMARIPFDVPAWEIALSMGLMLLTIVGAVWMAAKIYRTGILMYGKKTSYKELWKWLTY
                     KG"
     gene            4200..5378
                     /locus_tag="PKK03_05495"
     CDS             4200..5378
                     /locus_tag="PKK03_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNX83886.1"
                     /translation="MPRILVIDDERSIRNTLKDVLEYEKYEIDLAEDGTSGIELFGQQ
                     QYDAVLCDIKMAKMDGIEVLQKLQEVSSDVPVIMISGHGNIDTAVDSIKKGAFDFLEK
                     PLDLNRLLITIRNALERGSLITQTKVLKKQVSKKYEIVGNSPAIVKVKEMIDRIAPTE
                     ARVLITGANGTGKELVAHQIHEKSNRARGPFVEVNCAAIPSELIESELFGHEKGSFTS
                     AIKQRIGKFEQASGGTIFLDEVGDMSLAAQAKVLRALQENKITRVGSDKDIHVDVRVI
                     AATNKNIREEIDRNTFREDLYHRLSVILIHVPTLNERQEDIPLLADHFNTLICGEYGM
                     GKKKIKPDAIAELQKIIWTGNIREFRNVLERLIILCPEEITGRDVVAFAHPVSGFGEI
                     "
     gene            5375..7735
                     /locus_tag="PKK03_05500"
     CDS             5375..7735
                     /locus_tag="PKK03_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNX83887.1"
                     /translation="MSKRAVIPLLTVLTAALLVFTACGRKATDGIPLPFNDGWLFIRQ
                     DSANADKILPGIVPNHKWEEVTLPHSAVIEPLTVTATQWTGICWYKKLYRPDRRQRHR
                     NTGLLFDGAMHDAIVYLNGAEVARHTGGYLPFYVNLSDDLDYGRENEIMVKLDNRENP
                     HIPPGKPLSELDFLWYSGLYRNVNLYVTDRLHITAPPEYLSTLGGGILTGVRDVSRES
                     ATLALSVHISNDDHIPRSFRMKNTLLDAAGLEVASSLSELTDLEPGQGQRLETRMIVM
                     QPSLWTPATPFLYTIRTELLSDNGTVIDRRETRTGIRTVRITPDKGLLLNGESVRITG
                     TNRHQEYPYIGYALSDNASYRDAFKIREAGFNLVRCSHYPPAPAFLDACDELGLMVID
                     AIPGWQFMGDSLFAEATVRNTRMMCRRDRNHPSVVMWESSLNETVMPVSFLTRLHQTV
                     KDELPLGNNLTCSWMDTVCDVFIPARQHAGAPRYWKDYSKAKPLFISEYGDREYYAQN
                     AGFNQAEFSDLQPAERNSRQLRGDGDRRMLQQAYIFQEAHNDNLNGPGFGDANWLMFD
                     YNRGYAPDIEASGIMDIFRLPKFAWYFYRSQTEEGPVCFIAHYNLPSSGNTVRVYSNA
                     DTVKLFRNDTLIAVQGPDTDSNAVNLHHPPFTFILPDYLPGTLKATPLRNGKETASHS
                     VTTPGGPAIIRLSADFSNKPLRADNSDVIFVYATVTDSAGNQVHTSDAMVEFTVRGNA
                     SLIGDNPAKAEDGIASILLRAGNAGKIMIKASSPGISEAELMTESK"
     gene            complement(7765..10869)
                     /locus_tag="PKK03_05505"
     CDS             complement(7765..10869)
                     /locus_tag="PKK03_05505"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNX83888.1"
                     /translation="MRPGRILLFLLAVVLTSFLLSLLTVSRRQGKAIIPHGQESVVHD
                     SVQISGAGPEADGVLYDRPVEARLPVESGNNEVTPTAADATIPFYAGNAVRDSVAAGR
                     QVRIIYYGDSQIEGDRVTSALRRELREEGGGTGPGMISPVMPVMYTRSWVVRSSSNWK
                     RYTLLDYRNGTLPHNRLGPMLALCRFTPPGDSMQTRSFATVKISAVPGADPSVSQYDN
                     LRIFYGNNHDTVLVGIKSSSSLVDFAMMQMGEGPMEYSVPLPSVSEVTVEFTGRNSPD
                     IYALSLESTTGVIVDNVPVRGSAGLEFVMTDIRGFEGCYSDLKPDIIFLQFGLNVVRN
                     VRSEYHYYEEGLVKQVNYLKRASGGAPVVLVSVTDMALRDNDTIRRFPNIRAIRDAQK
                     VAATRSGAEFWDAWESMGGPGSILTWYNHKPPLSSKDLTHLSNEGTDTIAARIYSSLM
                     IPRPAPAPALVQPSQSVADSVSAGLKATADSMQARVTAQPDTAIFATGQDPGSADGTA
                     SGEETEETGISDGKKYSIVSQIIGWIASVLRYHPDQSFIFTTPAFWIFFLVVMAGFAL
                     LHRKRAMCHTWLLVVSLYFYYRAGGFFIILLLLTTLLTFYTAIMTGRAGTRGGKRFWL
                     VTNLVILLGFLSYFKYAGFFTDLINSVFDTTLVSRDIFSAWSNSLFGTNFNVSTIILP
                     VGISFFTFQALSYSIDVYRGRMAAERNIVDFAFYLTFFPQLVAGPIVRASEFIPQMHG
                     KYTISRNEFGYGLFLILQGLIKKMLISDFISTGFIDRVFDAPAIYSGFENLMAVYGYG
                     LQIYCDFSGYTDIAIGVAMLMGFRLPLNFNSPYKAANIGDFWRRWHISLSRWLKDYLY
                     IPLGGNRKGPLRTGINLMVTMLLGGLWHGAATRFVVWGGLHGMALVVEKIWHWLFRKV
                     SVRRRGAHIAGIFITFNFVSFAWIFFRAGTMEQAGVMLNQVFSSFSPGDYGLVLMSYL
                     PVVVLIASGYILHFLPVTVKESYRGLFIRLPLVVQVLVTLAVVLLLHKVGSEVVQPFI
                     YFRF"
     gene            complement(10883..12445)
                     /locus_tag="PKK03_05510"
     CDS             complement(10883..12445)
                     /locus_tag="PKK03_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNX83889.1"
                     /translation="MKKNLIKLVLPLLLSMVALPALAVNDTIIIRSDESTELIERDLD
                     SLLNNWFIRMSVAGTDISAADSVIYEFSDSIYKDRLSRINSIIIPPYNNIVRNHIHVY
                     TERKVDKFRVMLGMQDFYFPMIEDIFDSYGLPVELKYMAVIESALNPNAVSRVGATGL
                     WQFMYSTGRMYGLTINSVVDERRDPVKATHAAAKYLKDLYGIYNDWILVIAAYNCGPG
                     NVNKAIRRSGNRKDYWEIYYRLPRETRGYIPAYVAATYAMNYYREHNITPVQINLPLA
                     VDTVMVSTDMHLEQIAAVLDLPLEELRAMNPQYRTGLVPGRSRPSPVTMPLDRLGDFI
                     AMTDTITGYRKDQYLTTVNQTTMPQPTTSTYTPPDVSGKTKLTYTVKDGDNLGFISEW
                     YDVPLSELRYWNNIYRNTIRVGQKLTVYVNPAKAERYSKINGMTFAEKQQLEGKPVGT
                     TSLALASPQSSASASGDGDYEFYTVRYGDTVWDIARKFSGVSANDILSLNNISDASRI
                     MVGQKLRIRKKS"
     gene            complement(12450..13079)
                     /locus_tag="PKK03_05515"
     CDS             complement(12450..13079)
                     /locus_tag="PKK03_05515"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNX83890.1"
                     /translation="MKLSSTIAAFLLFVIALTCAAQPPDTVPVRKERARRYSIEPIRA
                     TMFAAALPGLGQIYNRKYWKVPIVYAGFGALGYAVTYNTKSYNTFINAYQDFNDIIPE
                     TDSYLELLVNLDPKTYDPVLFPKDYKPSTEAWVRTTLQNGVDYYRKYRDLSYIGIAAW
                     YLFTIIDAHVDASLFDYDITDDIRASVVPVNFNYSGVSPGITFALVKTF"
     gene            complement(13118..14005)
                     /locus_tag="PKK03_05520"
     CDS             complement(13118..14005)
                     /locus_tag="PKK03_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNX83891.1"
                     /translation="MNTNKKSPLGRGLGALIEGVEKEALEKKVEANLQIDINAIDGNP
                     FQPRTRFDSQSLEELAASIRQVGIVVPLTVRETGDGRYQLIAGERRLRAARMAGLTHV
                     PAYVRTADDTAMLEMALVENIQREDLDSIEVAITYQRLIEECSLTQEQLSDRVGKQRS
                     TVANYLRLLRLPAEIQLGIRNRNLTMGHARTLVNIEDPAKQINVFYHIIEEDLSVRAT
                     EDLVRHLQSAAAKDPAKIEKKKKLNTEYEELSKQLSSLFRSEVQFRINEKGRGKIVIP
                     FSDSEEMERIIGLLDKLNR"
     gene            complement(14023..14802)
                     /locus_tag="PKK03_05525"
     CDS             complement(14023..14802)
                     /locus_tag="PKK03_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX83892.1"
                     /translation="MSRIIALANQKGGVGKTTTAINLAASLAALEKKVLIVDADPQAN
                     ATSGVGIDLKQVKTTIYDCLIDGVNPRQAVLQCGIDNLSVIPSNIDLVGAEIEMLERP
                     EREKTLKKIIAPVAADYDYVLIDCSPSLGLLTVNALTAADSVIIPVQCEYFALEGLGK
                     LLNTIKIIQTNLNPALEIEGFLLTMYDSRLRLANQVVEEVTRHFQQMVFNTIIQRNVK
                     LSEAPSYGQPAILYDAESRGTVNYLNLARELLERVEARQKN"
     gene            complement(14947..16071)
                     /locus_tag="PKK03_05530"
     CDS             complement(14947..16071)
                     /locus_tag="PKK03_05530"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HNX83893.1"
                     /translation="MKRIAVYISLLSLALVAVISCSGSAGKRGTKAGEDAAAATDNDP
                     RPAKRLIEVLSPADNASYACTDRIVFSVAPAAGQVQADSVQVWVGSRLARTITTIPAT
                     AELDMNGDPGRISVRAVAFSQASRPQTVALFINLVSDITPVTYRYRVVKSFPHDRKAY
                     TQGLVYDNGFFYEGTGQQGESSLRKVEPETGKVLSQVSLDGSLFGEGVALLGDRIYQL
                     TWTSKVGFVYEKETLRQINRIYYQTQGWGLTTMGDSLVMSDGTNVIWFLDADFNVLSS
                     VDVWDNKGVVDNLNELEIIDGELWANIWQTDRIARIDPRTGKVLGYVELNNLLPREER
                     EPMTDVLNGIAWDAEGRRLFVTGKYWPRLYEITVLPASRN"
     gene            complement(16081..16485)
                     /locus_tag="PKK03_05535"
     CDS             complement(16081..16485)
                     /locus_tag="PKK03_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009161741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83894.1"
                     /translation="MKIAVDFDGTIVDHEYPAIGKEKLFAFITLKELQKRGALLIMWT
                     FREGKELQEAVDYCSQNGIEFYAVNRNYPEEQWTDGTPRKINVDIFIDDKNVGGFPGW
                     GEVLNMIDPWSDREEQAMREIKKRRFNLFGKK"
     gene            16576..17292
                     /locus_tag="PKK03_05540"
     CDS             16576..17292
                     /locus_tag="PKK03_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNX83895.1"
                     /translation="MIRHIPNTLTLLNLMCGFAATVCAIKGYNDYASYCIMAGMVLDF
                     SDGFAARLLKAYSALGKDLDSLADMVTFGIAPGSIVFNLLTAGGMTDLKAFAVAVIVP
                     AASALRLAKFNNDPGQSSSFRGLATPASAFTVVSVALAAAWTDCRIYDTMTGSPLFIS
                     LLALFLSVMMLLNARMFSLKFTSLKWKGNEERLIFVAVSLLLLAVFRITALPMIMAAY
                     ILISFIFPIAAGRRTSSGVM"
     gene            complement(17239..18363)
                     /locus_tag="PKK03_05545"
     CDS             complement(17239..18363)
                     /locus_tag="PKK03_05545"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNX83896.1"
                     /translation="MDIVQTGSWGFGEGPLIIAGPCSAESEEQLMATARALKATGRVH
                     LLRAGLWKPRSRPSSFTGEGFRALEWLRRVKAETGLPVAVEVASPEHARAAIEAGVDA
                     VWLGARTVSNPFSVDAIAAALRGTDMPVMVKNPLSPDLDLWVGAIERVYSAGIRKIAA
                     IHRGFTPYDKSRYRNLPKWELAIELRSRIPSLPVICDPSHMSGKASLVPETAQKALDM
                     NMAGLMIEVHHDPARALSDKEQQLTPDEFDKMMGSLLFRSSTSGDIGFINHLEELRNR
                     IDSIDHQLIGLLASRMRISEEMGEYKCRSGVTVFQLERWLEILESRTQHGISEGLDSE
                     FVGKVIRMIHEESIRCQDVVIKKLHDAGGCPPAGSDGENE"
     gene            complement(18392..19522)
                     /gene="cydB"
                     /locus_tag="PKK03_05550"
     CDS             complement(18392..19522)
                     /gene="cydB"
                     /locus_tag="PKK03_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNX83897.1"
                     /translation="MTLQISYLFLREYWWLIISLLGGLLVFLMFVQGGQSFIFSLPRN
                     DLQRKMVVNSLGRKWEFTFTTLVTFGGAFFASFPLFYATSFGGAYWVWMAILFSFIIQ
                     AVAYEYRSKPANVYGTRVFDTFLFINGCLGPFLIGVAVATFFTGSDFTLDLFNRVQWG
                     SGWHGLEALINPVNLLLGLAVLFLARVLGLMYLENSIDDKELTASLKKALVRNAIPFL
                     VFFLAFVAAILLGKGYRADPVTGEVTLVKYHYLHNLVGNVFTLVFFTGGVVLVLVALW
                     LGIFRGSRRAIWYAGFGTVPVVIALFLMAGYGETAFYPSSADPDSSLTLAKASSSYFT
                     LKTMMYVSFIIPFVVAYIWYAWSSINRSRITREELAGEDHTY"
     gene            complement(19535..21091)
                     /locus_tag="PKK03_05555"
     CDS             complement(19535..21091)
                     /locus_tag="PKK03_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNX83898.1"
                     /translation="MIENFDLSLVNWSRAQFALTAIYHWLFVPLTLGLSFILAFMETL
                     YVRTGDEMWKKITKFWMTLFGINFAIGVATGIILEFEFGTNWSNYSWFVGDIFGAPLA
                     VEGILAFFLESTFVAVMFFGWNKVSKKFHLASTWLVAIGANLSALWILVANAWMENPV
                     GMVFNPDTARNEMNSFREVVFNPVAIDKFLHTVTSGFVLASVFVVGISAWFLIRKREQ
                     ELARKSILIAGIFGLVSGLAVALTGDSSARTLAQVQPVKFAAMEALYEGKTDAGLTVI
                     GALTDSGERIGEKKVHDMKLKIEIPKLLSIMTTGKGEGYVPGLRDLIDGNPERGILSV
                     SEMMERGRYARQVLIDYKMAKELGDTESMASLAAKFEEKDFTENYFRYFGYSYFDHPW
                     QVIPSVPVTFYAFRVMVGLGFFFILLFVLAIWFHRRKTLERNPWFLWIALFSIPLAYL
                     ASELGWIVTEMGRQPWIIQNLMPVHVAVSNISAGAVQTTFWLFAVLFTALLIAEISIM
                     VRQIKTGPKH"
     gene            complement(21096..21323)
                     /locus_tag="PKK03_05560"
     CDS             complement(21096..21323)
                     /locus_tag="PKK03_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HNX83899.1"
                     /translation="MDSAGKTLNRIFRFYYDGFRTMSWWGKKVWIIILVKLFIMFLIL
                     RIFFFPDFLKVNFSNDRDRGDYVLEQLTGNN"
     gene            complement(21323..22459)
                     /locus_tag="PKK03_05565"
     CDS             complement(21323..22459)
                     /locus_tag="PKK03_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNX83900.1"
                     /translation="MNTTLRNILIIIGLALLVYLFWYFRNIIAYVLAAGVISLIGRPV
                     VDLLNGIRIWKFRFPKALSSLLTLLMLYGLLALFFVIFIPLVSSQIDALSGFDAGSIV
                     QGLRDQLDKLDTALRKVYRDMPADKTLYDIAVTTITNILNPASITDFAGNIVTVLRKL
                     IVAFVAITFLAFFFLKDEGLFKETIMVMVPDQYEKRIRNVLHSIKKLLIRYFIGIILQ
                     STVVLINITIGMTIVGFPFHQALVMGLLIGIFNIIPYVGPWLGGSVAVLMGVATAVTT
                     TGYPVIWLLIIYMVIVIACTQIIDNNLIQPMIYSRSVNAHPIEIFLVIFAVGSFAGIT
                     GMILAVPAYTALRVFAREFFYNFGPVRKITSGLGKENRETEPEA"
     gene            22696..23124
                     /locus_tag="PKK03_05570"
     CDS             22696..23124
                     /locus_tag="PKK03_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNX83901.1"
                     /translation="MLPMITRRNYKPLTLSDFLNEDFFPTFARSSTSLPAVNIREDEK
                     AFYLELAVPGMEKKDLKIEVKDDVLTISAEQKEEKQEDLEGYRRREFSYSSFCRSFYL
                     PEDVSGERIGATYKDGILNVEIPKLEEEKKKEKIRTISIS"
BASE COUNT         5736 a         6088 c         5960 g         5345 t
ORIGIN      
        1 gtaaactgtt tttatcatgg taaaaaagaa gagtcacgaa gagaggcatg atgaacccat
       61 gcctgagaag aaaacagcag ataatcaaat aaatacggag agcgatgctt ccgggcagca
      121 tgagcctgaa ggcgaacctg aagccacggt gaagagccag gcagaggaag aactgctcgg
      181 aaaactgaca gaaatgcagg acaggtactt acggctatca gccgaatttg acaattacag
      241 gaagagaacc ctgaaggaaa agatcgagtt gtcacagtca ggcggtgagt cggtaatcaa
      301 aaaacttctt cccgtcattg atgattttga cagggccatg aactccatgc gttccaccga
      361 tgactgcaat gcggtgaaga cagggctgga gctgatttac agcaagataa atgatttcct
      421 gaaacagaac ggagtgaagg agatagaggc catgaaagag ccgttcaaca gtgatttcca
      481 cgaggcagtc acaactgttc cggtggaaga tgataaactg aaagggctga tcatagacgt
      541 tacccagaag ggatatacac tgaatgataa agtgatccgc tttccgaagg tagtggtggg
      601 tgaataacag aatgcttctg cactgaaaca tgtcgaaaag agactattat gaagtactgg
      661 gcgtaagcaa gagtgcttcg aaggatgaga tcaagaaggc ttacaggaaa caggccctca
      721 agtatcatcc tgacaagaac cccggcaaca aggaggctga ggagaagttc aaggaggctg
      781 ccgaagctta cgaggttctc agcaatgatg agaagcgggc aaggtatgac cagttcgggc
      841 atgccggcat gaacggaggc gggttcgggg gcggattcgg cggaggcggg atgaccatgg
      901 aagacatctt ctcgtcattc ggtgacatct tcggtgacgc tttcggcggg gctttcgggt
      961 cacgtcgcgg cggcagccgc tcacgcagcg tcaacaaggg ctccaacctg aggctaaagg
     1021 tgaaactcac ccttgaggaa tttgctcacg gcgccgagaa gagggtcaag gtgaacaagt
     1081 atgtcacctg caatacctgc catggaagcg gagccgcttc agacagtgat gtgacgacat
     1141 gcaccgcctg caagggcacc ggccacacca ccagggttat gaacaccatc ctggggcaga
     1201 tgcagaccac cgcaccctgt aacgtctgcg gcggcgaggg caaggtcatc accaaaaaat
     1261 gcccgaagtg ctacggtgaa ggcatcacgc aggcagagga ggtgataccc ctttccatcc
     1321 cggcagggct tgccccgggc atgcagcttt ccgtgaccgg caagggcaac gcggcacgtc
     1381 gcggaggaat caacggagac ctgattgtag tcattgacga ggaaccccac gacgaactca
     1441 tccgtgaagg caatgacctc atatacaacc tcttcatcag catacccgat gcaatcctcg
     1501 gcgccaatgt agaggttccg accatcgacg gcaaggtcag gatcaagata gacccgggaa
     1561 cccagggagg caagatactc cgactcaggg gtaagggact gcctgatgtg aacgggtatg
     1621 gcaagggtga cctgctggta aatgtcaatg tctggatacc gaaaaacctt tcaagagagg
     1681 aacagaaagc tttcgaacag tttaaaaact cctcaacatt tgtcccgaag cccgatgccg
     1741 atgataaggg cttctttgac cgggtgaaga attacttctc ctgacagccg caaaacagca
     1801 ttataaaagg ttgatgcact tcagacagaa cgtatcagcc ttttttttat atctttgaaa
     1861 ttcccggact gaatacttta tttaattgtg gaaagacaat gatattattt gaagccaaag
     1921 aggttacgaa acgctttgct acccagacag cgcttgacag ggtcagcgta agcgttcccg
     1981 aacagagcat ttacggcctc ctggggccga acggagcggg caagacaacg cttatccgca
     2041 tcattaacca gatcacggcg cctgacagcg gcgccatatt cctgaacgga cgccctataa
     2101 accccgatga tgtaaacagc atcggctacc tgcctgagga acgcggtctg taccggaaaa
     2161 tgaaggtggg cgaacaggca ctctacctcg cacagcttaa gggactcagt catgccgaag
     2221 ccatgcggcg tcttaaatac tggttcaaaa agcttgctat catcgactgg tgggacaaga
     2281 aggtggagga gctgtcgaag ggcatgcagc agaaggtaca gttcgtcacc actgtgcttc
     2341 acgaaccaaa actgctcatc ttcgacgaac cattcacggg atttgacccg atcaatgtca
     2401 agcagataaa ggacgagatc ctcttcctcc gcgaacgcgg cgccactata attttttcaa
     2461 cccataacat gagctccgtt gaggaactgt gtgacaacat caccctcatc aacaaggcac
     2521 agaccatcct cgagggcagc gtggagaaaa tccgccatga ctgggcaggc aacgagttcg
     2581 aggttgtgtt cggcggggag gtgaacccct catccaacgg taactttaca ctcctttcct
     2641 cagtctttga cgagggtaag agcagggtgg tcatcaaggc atctgagcct gtcgacaata
     2701 atgccatcct cagggccatg accgctgccg gacaggtact ctcattcaac ccggccctcc
     2761 cgtcaatgaa cgagatattc ataagggtgg tggaaaccaa gaacaaggaa gctgtaaaaa
     2821 actgagccat gaacaagata gcacttatca tacgcaggga atatattacc agggtacgga
     2881 aaaagtcgtt cctcattatg accttcctta ccccgttcct ctttgcactt attttcatca
     2941 tcccggcact ggtgatgacc aatgaggaca aggatttcaa gaagatagca gtgattgaag
     3001 acggatctga cctcttcacc aatgtgatcc ctgacaccaa ggatactgac ttcgagtacc
     3061 tcgaaggggt gaaactgaat gacattaaga acaactttgc cgagctgggg tactacggtg
     3121 tgctctatat ttcccctgac ctggttgcca atcccgatgc catccagctc atatcgcgca
     3181 agcaaccgcc catagggctg cttgaccaca tctccggatc acttgagaag gagattgaga
     3241 agcagaagct gcttgcttac aacattgaga acctcgatga gatccttcgt accatcaaca
     3301 ccagcgtcag ggtgcagacc ataaagatgg atgagacggg cagcgcccgc gaaaccagca
     3361 caggcatttc gatggcgctg gcttacatcg ggggattcct catgtatatg ctggtattca
     3421 tgttcgggtc tatggtcatg cgtggtgtga tcgaggagaa gaccaaccgc attgtggaag
     3481 tgatagtatc atctgtgaag cccgtgcagc tcatgcttgg caagatcatc ggcgttgccc
     3541 tcgtgggact gacccagttc gcactctgga tagtgctgac cctcgcgctg gtcatggttg
     3601 tcaaaaccaa tctcctcccg gagggagcca tggaacagtt gcagcaactg ccccagagct
     3661 ttgccgaggc tgaccaggca acggcagccg gatcggcagg aaccacggtg acagcagagc
     3721 agctcaccga gtttcagcag atcttcgccg gcgccctcag catgccatgg ggactgatca
     3781 ttttcagctt cgtcttttat ttccttaccg gttacctgct gtatgcctct ctcttcgccg
     3841 ccatcggttc ggctgttgac aatgagacag agacgcagca attcatgctg ccggttacca
     3901 tccctattat actgggcctg gtggttgcca tgggaaccat gcagaaccct gagagttcac
     3961 tctcattctg gttctcaatc ataccgctca cctcccccat tgtgatgatg gcccgcatac
     4021 catttgacgt accggcctgg gagatagcgc tgtcaatggg gctgatgctt ctgacaatcg
     4081 tgggtgcagt atggatggca gccaagattt accgtaccgg gattctcatg tacggcaaga
     4141 aaacgtcata caaggaactc tggaagtggc tgacatataa aggataagaa tttgcggaca
     4201 tgccaaggat actggtcata gatgatgaaa gatccatcag gaacaccctg aaggatgtac
     4261 ttgaatatga gaaatacgag attgatctgg ccgaggatgg cacgtcaggg atcgaactct
     4321 tcgggcaaca gcagtatgat gccgttctct gtgacatcaa gatggcaaag atggatggta
     4381 tcgaggtgct gcagaagctg caggaagttt cttccgatgt gcccgtgatc atgatctcag
     4441 gccatggaaa catagatacg gcagttgact cgattaagaa aggagccttt gacttccttg
     4501 aaaagcccct ggatctcaac cgcctgctca taaccattcg caatgcgctt gagagaggct
     4561 cgctgatcac ccagaccaag gtactgaaga aacaggtcag caaaaagtac gagattgtgg
     4621 gcaactcgcc tgcaatagtg aaggtcaagg agatgattga caggattgct cccaccgaag
     4681 cccgtgtgct tatcaccggc gccaacggta cagggaagga gctggtggcg caccagatac
     4741 atgaaaagag caaccgtgca cgcggtccct tcgtggaagt caactgcgcg gcaataccgt
     4801 ctgaactgat tgagagcgag ctcttcggtc acgaaaaggg atcattcacc tctgcaataa
     4861 aacagcgtat cgggaagttt gaacaggcct cgggcggtac tatcttcctc gacgaagtgg
     4921 gcgacatgag cctggcagca caggcaaagg ttctcagggc actgcaggaa aacaagataa
     4981 cccgggtggg gtctgacaag gacatccacg tcgacgtcag ggtcatcgcc gcaaccaaca
     5041 aaaacatcag ggaggaaatt gaccgcaaca ccttcaggga agacctgtac caccgcctca
     5101 gcgtgatact gattcatgtg cccaccctta atgaaaggca ggaggacata cccctcctgg
     5161 ctgatcattt caacactctc atctgcggtg aatacgggat ggggaaaaag aagataaaac
     5221 ctgatgccat agctgagctg cagaagatca tctggaccgg caatatcagg gaattcagga
     5281 acgtgctcga aagactcatc atcctgtgcc ctgaggagat aaccggcagg gatgtggttg
     5341 catttgcaca tccggtatca ggattcggag agatatgagc aagagagccg tcatcccact
     5401 gctgacagtg ctgacggctg cgctgttggt cttcactgcc tgcggcagga aggcaactga
     5461 cggcatacct ctcccgttca acgacggatg gctctttatc aggcaggact ccgccaatgc
     5521 agacaagata cttccgggaa tagtccccaa ccacaaatgg gaagaggtga cgctgccaca
     5581 ctcagcagta attgaaccac tgacagttac agcgacgcag tggaccggca tatgctggta
     5641 taaaaagctc tacaggcctg acaggaggca gcggcacagg aacaccggac tcctttttga
     5701 cggagccatg catgatgcca tcgtttacct gaacggtgca gaagtagccc gtcacaccgg
     5761 cgggtatctt cccttctatg tcaatctgag tgatgatctt gattacggca gggaaaatga
     5821 gatcatggta aagctcgaca acagggaaaa tccgcacatc cctcccggca aacccctcag
     5881 cgaactcgat ttcctgtggt acagcggcct ttaccgcaac gtaaacctgt atgttactga
     5941 cagattgcac attaccgcac cacctgagta tctctccaca ctgggcggag gcatccttac
     6001 cggcgtcagg gatgtgagtc gcgagtcggc caccctggct ctctcagtgc atataagtaa
     6061 tgatgaccac atacccaggt cattcaggat gaaaaacacc cttcttgatg ccgccggact
     6121 ggaggttgcc tcctcgctgt cagagcttac cgacctggag ccgggacagg gtcagcgtct
     6181 cgagaccagg atgattgtga tgcaaccttc attatggacc ccggccactc ctttcctgta
     6241 taccatacgt accgagctac tctcagacaa cggcacagtg attgaccgcc gggaaacccg
     6301 caccggcata aggacagtga ggatcacacc tgacaagggt ctgctcctga acggtgagtc
     6361 tgtcaggatt acgggaacca acaggcacca ggaatatccg tatatcggat atgccctgtc
     6421 tgacaatgcc agttatcggg atgccttcaa gatcagggag gcaggcttca acctggtcag
     6481 atgctcccat tatcctccgg cacccgcatt tcttgatgcc tgtgatgagc ttggcctgat
     6541 ggtcattgat gccatccccg gatggcagtt catgggcgac tcgctttttg cagaggctac
     6601 tgtgcgtaat acacgcatga tgtgccgtcg tgaccgcaac cacccgtcgg tggtgatgtg
     6661 ggagtcatca ctgaatgaga ccgtcatgcc cgtcagtttc ctcaccaggc tccaccagac
     6721 ggtaaaggat gaactccccc ttggcaataa cctgacctgc agctggatgg atactgtctg
     6781 cgatgtgttc atcccggcac gtcagcatgc cggagctccc cggtactgga aggattacag
     6841 caaagcaaag cctctcttca taagtgaata cggcgaccgg gagtattatg cgcagaatgc
     6901 aggattcaac caggctgaat tttctgacct tcagcctgcc gaaagaaact caagacagct
     6961 gagaggtgat ggcgacagac gcatgctgca gcaggcatat attttccagg aggcgcataa
     7021 tgacaacctg aacggtccgg gattcggcga tgccaactgg ctgatgttcg actacaacag
     7081 gggctatgcc cccgatattg aagcctcggg catcatggac atattcaggc tgcccaagtt
     7141 cgcctggtat ttctaccgga gccagaccga agagggcccg gtatgcttta tcgcccatta
     7201 taacctgccc tcctcaggca atactgtaag agtgtacagc aatgctgaca ccgtgaagct
     7261 cttccgcaat gacacgctta ttgcggttca ggggcctgac actgacagca acgctgtcaa
     7321 tcttcaccat ccccccttca cattcatcct tccggattat ttgccaggga cattaaaggc
     7381 cactccactg cgcaacggaa aggagactgc atcacattca gtcaccaccc cgggcgggcc
     7441 tgcaatcatc cggctgtcag ccgacttcag caacaagccg ttgcgggctg acaattctga
     7501 cgttatcttt gtttacgcta cagtaacgga ttcagcgggc aaccaggtcc acacctccga
     7561 tgccatggtg gagttcactg tcaggggcaa tgcctccctg atcggtgaca acccggcaaa
     7621 agccgaagac ggaatcgcct ccatcctgct cagggccgga aatgccggca aaataatgat
     7681 caaggcttct tctccgggaa tttcggaagc agaattgatg actgagtcaa aatgactccg
     7741 cttcccgggt taccgggaaa acaatcagaa acggaaatag atgaatggtt ggaccacctc
     7801 tgatcccacc ttgtggagaa gcaataccac agcaagggta accagcacct gtaccaccag
     7861 cgggagcctg ataaagagtc ccctgtatga ctccttgact gttacgggca ggaagtggag
     7921 gatgtatcct gaagcgatca gtacaacgac aggcaggtat gacatcagca ccaggccata
     7981 gtctcccgga gaaaacgatg agaatacctg gttaagcatc accccggcct gttccatggt
     8041 gccggccctg aagaatatcc atgcgaagct gacaaagttg aatgtgatga agatcccggc
     8101 gatatgtgcg ccacgtcttc ttactgacac ctttctgaag agccagtgcc agatcttttc
     8161 cacaaccagt gccatcccgt gcagtccgcc ccagaccacg aaacgtgtgg cagccccgtg
     8221 ccacagaccg ccgagcaaca tggtgaccat caggttgatg ccggttcgca ggggaccttt
     8281 cctgtttcct ccgaggggaa tataaagata atccttcagc cacctcgaga gggatatatg
     8341 ccacctcctc cagaaatcgc ctatgttggc agccttgtaa ggcgagttaa agttcagcgg
     8401 aagcctgaat cccatcagca ttgcaacgcc gatggcgata tcggtatagc ccgagaagtc
     8461 acagtagatc tgcaagccgt acccgtacac cgccatcagg ttctcaaaac ccgaatagat
     8521 ggccggggcg tcaaagaccc tgtcaatgaa tccggtggag atgaaatcgg agatcagcat
     8581 cttcttgatc aatccctgga ggatgagaaa caatccgtac ccgaactcgt tgcggctgat
     8641 ggtatacttg ccgtgcatct gcgggatgaa ctctgacgcc cttacgatag ggcctgccac
     8701 cagctgagga aaaaaggtca ggtaaaatgc aaaatcgact atgttccgtt ctgctgccat
     8761 ccgcccccgg tatacatcaa tgctgtagct gagcgcctgg aaggtgaaga atgagatgcc
     8821 caccggcagt atgattgtgc tgacattgaa gttcgttccg aacaggctgt tcgaccaggc
     8881 tgagaagatg tcacggctga ccagggtggt gtcgaacaca gagttgatca ggtctgtgaa
     8941 gaacccggca tatttgaagt atgagaggaa accaagaaga atcacaaggt tggttaccag
     9001 ccagaacctt ttgcctcccc tggtacctgc ccggccggtc atgatggcgg tgtagaaggt
     9061 aagcagggtg gtgagcagta aaagtatgat gaagaatccg cctgcacggt aataaaaata
     9121 gagactgaca accagcagcc atgtgtggca catggccctc ttcctgtgca gcagggcgaa
     9181 ccccgccatc accacgagga agaatatcca gaaggcaggt gtggtaaaga tgaatgactg
     9241 gtcggggtgg tacctgagta ctgacgcaat ccagccaata atctggctga cgatgctgta
     9301 tttttttccg tctgagatcc cggtttcttc agtctcctcc ccggatgcgg ttccgtctgc
     9361 tgaaccggga tcctggcctg tggcaaatat tgcggtgtca ggctgggccg taacgcgggc
     9421 ctgcatggaa tcagcagtgg ctttcagacc ggcggaaact gaatctgcga ctgactgcga
     9481 cggctgtacc aacgctggtg ccggagcagg ccggggtatc atgagggatg aataaatcct
     9541 ggcagcaatg gtgtctgtcc cttcgtttga aaggtgtgtc agatccttcg atgacagcgg
     9601 aggcttgtga ttgtaccagg tgagtattga tcccgggccg cccatagact cccatgcgtc
     9661 ccagaactct gccccgctgc gggttgcggc caccttctgt gcatcgcgga tggccctgat
     9721 gttcgggaac cggcgtatgg tgtcattatc cctcagggcc atgtcagtca cgctgaccag
     9781 caccacaggc gccccgccgg aggccctctt caggtaatta acctgcttta ccagtccctc
     9841 ttcatagtaa tggtactcgc ttctgacatt ccttaccacg ttcaggccaa actgcaggaa
     9901 gatgatgtca ggcttcaggt ccgaatagca tccttcaaaa ccccggatgt cagtcattac
     9961 aaactccagc ccggcgctgc cgcggacggg cacgttgtct acgatcacgc ccgtggtgct
    10021 ctcgaggctc agtgcgtaaa tgtccggtga attgcgtccg gtaaactcca ccgtcacctc
    10081 ggagactgac ggaagtggga ccgagtattc catcggcccc tcgcccatct gcatcattgc
    10141 aaaatcaacc agtgagctgc ttgactttat gccgacgaga actgtgtcat gattgttgcc
    10201 gtagaatatc ctgaggttgt catactgaga tactgaggga tcagcgcccg gaacggcact
    10261 tattttcacg gtggcaaagg aacgtgtctg catgctgtca cccggagggg tgaaacggca
    10321 cagcgccagc atgggaccca gtctgttgtg gggcaatgtc ccgttcctgt aatcaaggag
    10381 cgtgtatctc ttccagtttg atgaggagcg taccacccat gacctggtat acatcaccgg
    10441 catcaccggg gagatcatgc ccggaccggt gcctcctccc tcctccctca gctcacgcct
    10501 gagggctgag gtgacacggt caccctcaat ctgtgagtcg ccgtaataga tgatccttac
    10561 ctgcctgcct gcagcaactg aatccctgac tgcattgccc gcataaaagg gaatggttgc
    10621 gtcagccgcg gtgggtgtga cttcattatt tccggattca accggcagtc gggcttcaac
    10681 aggcctgtca tataatacgc cgtcagcctc cggcccggca ccggatattt gtacactgtc
    10741 atgtacgaca ctctcctgcc catgcggtat gatcgccttt ccctgtcgcc ggctgacggt
    10801 cagcaggctc aggaggaacg atgtcagaac gacagcaagc agaaatagca ggattctgcc
    10861 ggggcgcatt tttctttatt attcagcttt tctttctgat ccgcagcttc tgacccacca
    10921 ttatccttga agcatcggag atattattca ggctgaggat gtcattggct gaaacaccac
    10981 tgaacttcct ggctatatcc cacaccgtat cgccatacct gacggtatag aactcgtagt
    11041 caccatctcc ggacgctgag gctgatgact gcggtgatgc cagtgccagt gatgtggttc
    11101 ccacaggttt gccctcgagt tgctgcttct cggcaaaggt catgccgttg atcttgctgt
    11161 aacgctctgc ctttgcagga ttgacgtata ccgtaagctt ctgccccacc ctgatggtgt
    11221 tgcggtagat gttgttccag tatctcagct cagaaagggg gacatcatac cactccgaga
    11281 tgaatccaag attatctccg tccttgacgg tgtatgtcag cttggtcttg ccgctgacat
    11341 caggcggtgt atacgtcgag gttgtgggct gtggcatggt ggtctggttg accgtggtca
    11401 ggtactggtc tttcctgtag cccgtaatgg tgtcagtcat tgcaatgaag tcacccagcc
    11461 tgtcaagagg catggtgacg gggctgggcc tgcttcgtcc ggggacaagg cctgtgcggt
    11521 attggggatt catcgccctg agctcctcca gcggcaggtc aagcactgct gctatctgct
    11581 caagatgcat gtcggttgac accattactg tgtcaactgc aagcggcaga ttgatctgca
    11641 cgggagtgat attatgctca cggtagtagt tcatcgcata ggtggccgcc acatatgccg
    11701 gtatgtaacc acgggtctca cggggcaggc ggtagtatat ctcccagtaa tccttcctgt
    11761 ttcctgaccg gcggatagcc ttgttgacat tgcccggtcc gcagttgtag gccgcaataa
    11821 ccaggatcca gtcattgtag ataccgtaca gatccttcag gtattttgct gcagcgtggg
    11881 ttgccttgac cgggtcacgc ctctcatcca ccactgaatt gatcgtcaga ccatacatcc
    11941 ttcctgtgct gtacatgaac tgccacaatc ccgttgcacc tacccggctg acggcatttg
    12001 gattaagagc cgattcaatg actgccatgt atttaagctc caccggcaac ccgtaagaat
    12061 cgaagatatc ctctatcatc gggaaataaa agtcctgcat gcccagcata acccggaact
    12121 tgtccacctt tcgttccgtg taaacatgga tgtggttgcg gacaatattg ttatagggcg
    12181 gaattataat ggagttgatt cggctgagac ggtccttgta gattgaatca ctgaattcat
    12241 aaataaccga atcggcagcg gaaatgtcgg tgccggcaac tgacatcctg atgaaccagt
    12301 tattcaggag gctgtctaga tcacgctcta tcagctccgt tgattcatca cttcttatta
    12361 ttattgtatc attaaccgcc agggcgggaa gcgccaccat cgacagcagc agcggaagga
    12421 cgagttttat cagattcttc ttcataaaat taaaacgtct ttaccagggc aaatgttata
    12481 ccgggagaca ctccggaata attaaaattc accggtacaa ctgaagccct gatgtcatcg
    12541 gttatatcat agtcaaaaag gctggcatcg acatgcgcgt caattatggt aaaaaggtac
    12601 catgccgcaa tgccaatata tgaaagatca cggtacttcc tgtaatagtc aacaccgttc
    12661 tggagggtgg ttctgaccca tgcttcggtg gaaggtttgt agtcttttgg gaaaagcaca
    12721 ggatcatagg tctttggatc cagattaaca agcagttcga ggtaactgtc agtctccggg
    12781 atgatatcgt tgaaatcctg gtatgcgttt ataaaagtgt tgtagctttt ggtgttgtag
    12841 gtgactgcat agccaagtgc gccgaaaccg gcatagacta tcggcacctt ccagtattta
    12901 cggttgtaga tctgaccgag gcccgggagt gcggcagcga acatggtagc cctgatgggt
    12961 tcaattgaat atctcctcgc cctctctttt cttacaggaa cagtgtcagg cggctgggcc
    13021 gcgcaggtta aggcaatgac aaagagaaga aaagctgcta tagtgctgct gagcttcaag
    13081 ggcaaacatt ttcgtttgcc ggcaaatata aataaaatta cctgttaagt ttgtcgagca
    13141 atcctattat gcgttccatc tcctcgctgt cagagaatgg aattacgatt ttcccgcgcc
    13201 ctttttcatt tatccggaac tgtacttctg accggaaaag gctgctgagc tgttttgaca
    13261 gctcttcgta ttcggtgttg agcttcttct ttttctcgat ttttgcaggg tcttttgccg
    13321 cggcgctctg gagatgcctg acaaggtctt ctgttgcccg tacagacaga tcctcctcga
    13381 tgatatgata gaaaacatta atctgtttag ccggatcttc gatgtttacc agggttcgtg
    13441 cgtgacccat ggtgaggtta cggttgcgga tgccgagctg tatctctgcg ggcaggcgta
    13501 gcaggcgcag gtagttggcc accgttgaac gctgcttccc taccctgtca ctgagctgtt
    13561 cctgggtcag gctgcattcc tctataaggc gctggtaggt tattgcaacc tcaatggaat
    13621 cgaggtcttc cctctgaata ttttccacga gggccatctc gagcatagcc gtgtcatcag
    13681 ccgtgcggac atatgcaggc acatgtgtca gtccggccat gcgcgctgcg cgaaggcggc
    13741 gttcaccggc aatgagctgg taacggccgt cgcctgtctc acggactgtg agcgggacaa
    13801 caatgcccac ctggcggatg gaggcggcga gctcctcgag cgactggctg tcgaaacgag
    13861 tacggggctg aaaaggattt ccatcaattg cgttgatgtc tatctggagg ttagcctcca
    13921 ccttcttttc gagagcctct ttttcaacac cttcaataag ggctccgaga cctctgccca
    13981 gcgggctctt tttgttcgtg ttcatggtgc ggataaacag ttttagttct tctgcctggc
    14041 ttccaccctc tcaagcagtt cacgggccag gttgagataa ttgaccgtac cccttgattc
    14101 agcatcataa agtatggcag gctgcccata gctgggagcc tccgagagct tcacattgcg
    14161 ctgtatgatt gtattgaaga ccatctgctg gaaatggcgc gtgacttcct ccaccacctg
    14221 gttggccagg cgcaggcgcg agtcatacat ggtcagcagg aatccctcaa tctcaagcgc
    14281 cggattcagg ttggtctgaa tgatcttgat agtattgagc agcttgccca gaccctccag
    14341 cgcaaaatac tcacattgaa cgggaatgat cactgagtcg gcagccgtga gtgcattgac
    14401 tgtcagcagg ccgagcgacg gggaacagtc tatcagtacg taatcataat cagcagccac
    14461 aggtgctatg atcttcttaa gtgtcttctc cctctcgggc ctctcaagca tctctatctc
    14521 cgcccccaca aggtcaatat tggaaggtat gaccgaaaga ttgtcaatgc cgcactgcag
    14581 gacagcctgc cttggattga ccccgtcaat caggcagtcg taaatggttg tcttcacctg
    14641 cttgaggtca attcccaccc ctgaggtggc attggcctgt gggtcagcat caacaatgag
    14701 aaccttcttt tcaagcgcgg ccagactagc ggccagattg attgcagtgg tcgtctttcc
    14761 tactcctccc ttctgattgg ccagtgctat tatccttgac atatgatccc ttttttcctt
    14821 aaattaatga gcttcaaatt tacaattaat gaccttgccc gaaagccggc cccatatgtt
    14881 attgtaaaca attttttcgg ggttattaac acgttttgaa cagccggtgt ggctttcccg
    14941 gccgcttcag ttgcgcgatg cgggcaagac agttatctca taaaggcgcg gccagtattt
    15001 gcccgtgaca aagagtcgcc tgccctcagc atcccaggca ataccgttaa gcacatccgt
    15061 catcggctcg cgctcctcgc gcggaagaag gttattcagc tcaacatatc ccagtacctt
    15121 cccggtacgg ggatcaatcc gtgcaatcct gtcagtctgc catatgtttg cccatagctc
    15181 gccgtcaata atctcaagtt cattcaggtt gtcaacaaca cccttgttgt cccacacatc
    15241 aaccgaggag agaacattga aatcagcgtc aaggaaccag atcacgtttg ttccgtcact
    15301 catcaccagg ctgtcgccca ttgttgtaag cccccatccc tgggtctgat aataaatgcg
    15361 gtttatctgg cggagagtct ccttctcata tacaaacccg accttgctgg tccatgtgag
    15421 ctggtagatc cggtctccca gcagcgccac cccctcaccg aagagtgagc cgtcgaggct
    15481 gacctggctg agtaccttgc ctgtctccgg ctctaccttt cttagtgacg actctccctg
    15541 ctggccggtt ccctcataaa agaacccgtt gtcatatacc aatccctggg tataggcttt
    15601 cctgtcgtgc ggaaaacttt tgactaccct gtagcggtac gtaacaggag tgatgtccga
    15661 gaccaggtta atgaagaggg ccaccgtctg gggccttgag gcctgggaga atgcaactgc
    15721 cctgacagag atcctgcccg gatcaccgtt catatcaagt tccgccgtgg cgggaatggt
    15781 tgttattgtc cgtgccagcc tgctccccac ccacacctgt acggagtctg cctgtacctg
    15841 acctgcagcg ggggctacgg aaaaaacgat cctgtccgta caggcatagg aggcattgtc
    15901 agcaggtgac agcacctcga tcagtctctt tgccggcctc gggtcattgt ctgtggcagc
    15961 agcagcatct tcaccggcct ttgttccgcg cttcccggcc gagccggaac atgagatcac
    16021 ggctacaagc gccagactca gcagtgagat gtaaacagca attctcttca tgggttttgt
    16081 tcattttttg ccaaacagat tgaagcggcg cttttttatc tcacgcatcg cctgctcttc
    16141 cctgtcggac catgggtcaa tcatgttgag aacctcgccc catcccggga aaccgccaac
    16201 gttcttgtca tcaatgaaga tatccacatt gatcttcctc ggggtaccgt ctgtccactg
    16261 ttcctcgggg tagttgcggt tcacggcata gaattcgatt ccgttctgac tgcagtagtc
    16321 gacggcttcc tgcaactcct taccctcacg gaaggtccac atgatgagca gcgcccccct
    16381 cttctgaagc tccttcagag tgatgaaggc aaaaagcttc tccttgccga tggcggggta
    16441 ctcatgatca acaatggtgc cgtcaaagtc aactgctatt tttatgttag tcaggtctct
    16501 cataatccga aagcaaaaat aaacaaataa tagttgccac tattattgta atttagcgat
    16561 gccacaaaaa gagttatgat aagacatatc ccgaacaccc ttacccttct gaacctgatg
    16621 tgtggttttg ccgcaacagt ctgcgccata aaaggttata atgattatgc atcatactgt
    16681 atcatggccg gaatggtcct tgatttttca gatggttttg cggcgaggct gctgaaggca
    16741 tattccgccc tgggaaaaga cctggacagc cttgctgata tggtcacctt cgggatcgct
    16801 cccggttcca tcgtattcaa cctccttact gcaggaggca tgactgacct gaaagccttt
    16861 gcagttgcag tcatcgtacc tgccgcctcg gcgctgaggc ttgcaaagtt caacaatgac
    16921 ccgggccagt catcatcatt caggggattg gccacaccgg caagtgcctt cacggtggtc
    16981 tcggtggcgc ttgccgctgc atggactgac tgccgcattt acgacacgat gaccggatca
    17041 cccctgttca tcagcctcct ggctcttttc ctgtcggtaa tgatgctgct gaatgcacgg
    17101 atgttttcac tcaagttcac cagcctgaaa tggaagggaa atgaggagcg cctcatcttt
    17161 gtagcggtga gtctcctgct gcttgctgtg ttcaggatca cggcgctgcc gatgataatg
    17221 gccgcctata tcctcatttc attcattttc cccatcgctg ccggcaggag gacatcctcc
    17281 ggcgtcatgt agctttttta tcaccacatc ctggcaccgg atggattcct catgaatcat
    17341 ccggatcact ttgcccacaa actcactgtc aaggccctcg ctgatgccat gctgtgtcct
    17401 cgactccaga atctccagcc atcgctcgag ctggaagacg gttacgccgc tgcggcactt
    17461 gtactctccc atctcctctg atatgcgcat cctcgaggcg agcagtccga tcagctgatg
    17521 gtcaatggaa tctatcctgt tgcgcagttc ctcgagatga ttgatgaacc cgatatctcc
    17581 gctggtggat gaacggaaga gcaggcttcc catcatctta tcaaattcgt caggagtaag
    17641 ctgctgctcc ttatcgctca gcgcacgggc agggtcatgg tgcacctcaa tcatcagtcc
    17701 cgccatgttc atgtcaagcg ctttctgtgc cgtctccggt accaggcttg ccttccccga
    17761 catgtggctg ggatcacaga tcacaggaag ggatggtatc cggctccgga gctcaattgc
    17821 cagctcccac tttggaagat tgcggtagcg gcttttatcg tagggagtga atcccctgtg
    17881 aattgctgct atcttgcgga ttcctgcgct gtatactctc tcgatggctc ccacccacag
    17941 atccaggtca gggctgagcg ggttcttgac catcacgggc atatctgtcc cccgaagggc
    18001 tgcagcgata gcgtcaacgc tgaacgggtt cgacactgtt cgtgccccga gccacaccgc
    18061 atcaacgcct gcctcaatgg cagcacgggc atgctcaggt gatgccacct ccaccgccac
    18121 agggagcccc gtctccgcct ttaccctgcg gagccactcc agcgccctga atccctcacc
    18181 cgtgaatgaa gagggccttg aacgcggttt ccataatccg gccctcagca gatgaaccct
    18241 gccggtggcc ttcagcgccc tggccgtggc cattagctgt tcctcgctct cggcgctgca
    18301 cggtccggca attatcagag gcccttcgcc aaaaccccac gatcccgtct gcactatatc
    18361 cattgacact tccgcgacaa tatttaaaaa atcagtatgt gtgatcctct ccggccagtt
    18421 cctccctggt tatccggctg cggttgatcg agctccaggc ataccatatg tacgccacca
    18481 caaacggtat gatgaacgaa acgtacatca tcgtcttcag ggtgaagtaa ctcgatgatg
    18541 ccttagccag cgtcagtgaa ctgtcagggt cggcagatga gggatagaag gctgtctctc
    18601 cgtagcctgc catcaggaac agggcaatta ccaccggcac cgttccgaaa ccggcatacc
    18661 atattgctct tcgcgaaccc ctgaagatgc ccagccagag cgctacaagg accagcacca
    18721 ctcctcccgt gaagaaaacc agggtgaaga cgttgccaac caggttatgc aggtaatgat
    18781 attttacaag tgttacctct ccggtgacag gatcagccct gtaacctttc ccgaggagaa
    18841 tagccgcgac aaacgcaagg aagaagacca ggaacggaat ggcatttctc accagcgcct
    18901 tctttaatga tgctgtcagc tccttatcat cgatgctgtt ttcaaggtac atcagtccca
    18961 gcaccctggc caggaacagc accgctagtc caagcagcag gttgacaggg ttgatcagtg
    19021 cttccaggcc atgccagcct gagccccact gcaccctgtt gaaaaggtcg agggtgaagt
    19081 cagagcctgt gaagaatgtg gccacggcca ctccgataag gaacgggccc aggcagccgt
    19141 tgatgaagag gaaggtgtca aagacgcgtg tgccgtagac gtttgcaggc ttggagcggt
    19201 actcgtaggc aaccgcctgt atgataaagc tgaacaggat cgccatccaa acccagtatg
    19261 cgcctccgaa gctggttgca tagaacagcg ggaaagaggc aaagaaggcg ccaccgaatg
    19321 tcaccagggt ggtgaaggtg aactcccact tccggccgag ggagttgact accatcttcc
    19381 gctgcaagtc gtttcgcgga agcgagaaaa taaacgactg tcctccctga acgaacatga
    19441 ggaagacgag aaggccgccg agaagtgaaa taatcagcca ccagtactca cgcaggaaca
    19501 aataagatat ctgcaatgtc ataatccttt cctcctaatg ttttggtccg gtttttatct
    19561 gtcttaccat aattgaaatc tctgcgatca gcagggcggt gaacagcact gcaaagagcc
    19621 agaatgttgt ctggacagcg cccgcactga tatttgacac ggccacatga acaggcatca
    19681 ggttctgtat aatccagggc tgccgtccca tctcggtgac tatccagccg agttcactgg
    19741 ccaggtaagc cagcgggatt gagaagaggg ctatccagag gaaccaggga ttcctctcca
    19801 gggtctttct tcggtggaac cagatggcaa gcacaaaaag caggataaag aagaagccca
    19861 ggcccaccat caccctgaag gcatagaacg tcaccggaac agaaggtatg acctgccatg
    19921 gatgatcaaa atagctgtac ccgaaatacc tgaagtagtt ttcggtgaaa tccttctctt
    19981 caaatttggc tgccagcgat gccattgatt cagtgtcccc gagttccttt gccatcttgt
    20041 agtcgataag cacctgcctt gcgtagcgtc ctctttccat catttcgctg acagacagaa
    20101 tacctctctc aggattgccg tcaatcaggt ccctgaggcc ggggacgtag ccttcgccct
    20161 tgccggttgt catgattgag agcagtttgg ggatctcaat cttcagtttc atgtcatgta
    20221 ccttcttttc gccgatccgt tcgccgctgt cagtaagtgc gccgataacc gtgaggcctg
    20281 cgtcggtttt tccctcatac agagcctcca ttgcggcaaa tttaaccggc tgtacctgtg
    20341 ccagtgtccg tgccgaagag tctcccgtca gcgccactgc cagtcctgac accaggccga
    20401 atatgcctgc aataagtata ctctttcgtg ccagttcctg ctctctcttc cttatcagga
    20461 accatgcgct tatgcccaca acaaataccg atgccaggac aaatcctgat gtcaccgtgt
    20521 ggaggaattt gtctattgca acaggattga agaccacttc gcggaagctg ttcatctcat
    20581 ttcgggcggt atcaggattg aataccatgc caaccgggtt ttccatccat gcattggcaa
    20641 ccaggatcca gagggctgaa aggttcgcgc ctatggcgac gagccaggtg gaggccagat
    20701 ggaatttttt gctcaccttg ttccagccga agaacatgac agccacgaag gtcgattcga
    20761 ggaagaaggc cagtatgcct tctacagcca gcggcgcccc gaagatgtcg cccacaaacc
    20821 aggaatagtt cgaccagttg gtgccgaatt caaattcaag tatgatgccg gtggccaccc
    20881 cgatggcgaa gttgatgccg aagagtgtca tccagaactt tgttatcttc ttccacatct
    20941 catcaccggt gcgcacatag agagtctcca tgaaggcaag gatgaatgaa aggccgagcg
    21001 tcagaggtac gaagagccag tgatagattg ctgtcagtgc gaactgagcc ctcgaccagt
    21061 ttactagtga caggtcaaag ttttcaatca tattgtcagt tatttccggt tagttgttca
    21121 agtacataat cacccctatc cctgtcattg ctgaaattaa ccttcagaaa atcagggaag
    21181 aagaatatcc tgagtattaa aaacatgatg aagagcttga caaggatgat gatccagacc
    21241 tttttccccc accatgacat ggtccggaaa ccatcataat agaatctgaa tatcctgttg
    21301 agtgttttgc ctgcactatc cattatgcct ccggttctgt ttccctgttc tcttttccca
    21361 ggcccgatgt aatctttctc accggcccga agttatagaa gaactcgcgg gcgaagaccc
    21421 tgagtgcggt atatgccggc actgccagaa tcattccggt gattcctgca aagctgccaa
    21481 cggcgaagat gaccaggaag atctcaatcg ggtgggcatt gacgcttcgc gaatagatca
    21541 tcggctggat aaggttgtta tcaatgatct gggtacaggc gatgacgata accatataga
    21601 ttatcagcag ccagatcaca ggataacctg ttgtggtcac cgcggtggcc acacccatga
    21661 gaactgcaac ggaacctccg agccaggggc cgacataggg aatgatgttg aagatcccta
    21721 tcagcaggcc catcaccagt gcctggtgga acgggaaacc gacaatggtc atcccgatgg
    21781 tgatgttgat aagcaccacg gtgctctgaa gaatgatacc tataaaatac ctgataagca
    21841 gtttcttgat tgaatgaagt acattcctga tccgcttttc atactggtcc ggcaccatca
    21901 ccatgatcgt ctccttgaac agcccctcat ccttgaggaa gaagaatgcg aggaaggtaa
    21961 tagccacgaa ggcgacgatg agcttgcgca atacggtgac aatatttccg gcaaaatcgg
    22021 taatggaggc cggatttaga atgttggtta ttgtggtgac cgctatgtca taaagggttt
    22081 tgtcagccgg catatccctg tatacctttc tcaatgccgt atcaagcttg tcaagctggt
    22141 cacggagacc ctgtacaatg gaaccggcgt caaaacctga aagggcatca atctgactgc
    22201 tcaccagcgg gatgaagatg acaaagaaca gtgcaaggag cccatacagc atcagaagag
    22261 tcagcagcga gctgagtgcc ttcggaaacc tgaatttcca gatacggata ccattcagca
    22321 gatcaaccac cggcctgcca atgagtgaga tcaccccggc agcaagaaca taggctatta
    22381 tgttcctgaa ataccagaaa agataaacca gcaacgccag tcctataata atgaggatat
    22441 tacgcagggt agtattcatt tttcaacagg tctcaggtaa ttggttttcc gtacttcacc
    22501 gtgcggatta aacactgcaa tttcaaaatt aggaataatt cccggtttaa caacctgatc
    22561 ttataaaatg ccaatatgtc atgacttatt tttgattttg tgccattatg gcaccatttt
    22621 aagagcggca catgaattga aataatgagg gtgtaaatgc aaataaagtt taacaatata
    22681 aaaaaggagg ttgctatgtt accaatgatt accaggagga attacaagcc gcttacgttg
    22741 tcggatttcc tcaatgaaga tttcttcccc acatttgcca ggtcgtcaac cagtctgcct
    22801 gcagtgaata tcagggaaga tgagaaggcg ttttatcttg agcttgccgt tcccggcatg
    22861 gagaagaagg acctgaagat tgaagtgaag gatgatgttc tcaccatttc tgccgagcag
    22921 aaggaggaga agcaggaaga tcttgaggga tacaggcgca gggagttcag ttacagctca
    22981 ttctgccgca gtttctacct gcctgaggat gtcagcggtg aaaggatcgg tgccacctat
    23041 aaggatggta tcctgaatgt tgagataccc aagcttgaag aagagaagaa gaaggaaaag
    23101 atcaggacaa tctcaatctc ataatttgc
//
LOCUS       DAOPXU010000038        23001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_2249_length_23001_cov_13.529983, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000038 DAOPXU010000000
VERSION     DAOPXU010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23001
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2249_length_23001_cov_13.529983"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..9074
                     /locus_tag="PKK03_05575"
     CDS             12..9074
                     /locus_tag="PKK03_05575"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83902.1"
                     /translation="MRFTNLNYPIYNAFPPTTSGATSGAATVYFEGASDNILTCNGVT
                     DFYNLIVNKGTDQTYGLTVSSSAYPNFRIFGANDQEAESVSGNPAMRKALWIYAGTLV
                     LKGNIFIPSLSEGSLANAHYYIPSNGALICDGVDVVVFVTADDYREVNTAYGVSASDN
                     VAMGINTAGTGSSLYVFGRLGVNNGVLSAKESGGIVTSATASGQIIINGGVVDAKQFL
                     SSSGSASYSQSGGLLILRGRFRRTPVVYTTITGLTDMTAASLNTSRTTSGISALHGSF
                     NLENTDNIYTVSGGTIRIYDVTTTATAEAFDVKSSPANINVTGGTLEIIPTGGSGTDA
                     TSYSIYSTAPLHNLIINRASGAAVVRMSTPLNVTASLSVISGALNANSSDLSVGGNFL
                     LETGTTYTTGTNTTTFNGSSAQLFTINHASPLSLSSFSVTKPGGVILALAGTQNTVNV
                     SSALNIVAGTLNDGGKILNVSGNIFNSGLATGTGKIVLNGTTAQTINGGGTFGNLELA
                     NTSATPVSLLNRTTVNGDLNFAVNGNFNIGTYNLHLNDTASILNAGTARFIQTAGNAG
                     DGGLSRSYSSNDSFIFPVGMTGRYTPATIGFTASPAAYGSVSVNPVDYEHPVTTVNGQ
                     SLSYYWRVKSTGFSGIPLFSVSHIFVYSQTDVAGTESSYVPALYDNVSRSWYIGRTSD
                     INISTNTISDWATPTNSTNFLDGDYTAGSAGSFGLPRIYYSRQNGLWGSTSTWSLTGH
                     TVTNPPSTAPGANDIVIIGGNDSIWLATETPPLPPAPGYQPASYYQRNKAVVNCATLQ
                     IEAASVLDIQNNPGSTFASVLSHPNGNGKIRITTRDASNFENSEPFVYPSGDFSEFSI
                     NDGISEFYTINPRAGAYYILPSNATEYGSVIMTPLKGSNIIMPNLPQVTVNGDLICNG
                     SDADAWLAMSWSGTDYGTIGEKTVNVMGNLIVAGGSFGFIYNGTTLQRINIDGNVYVA
                     PGAGIDVWNSSTNNIMSIGGSVFNNSNNTTAPSGSPSVMRFINSGNRCDLIFTGNRST
                     IVTNNPALSTTPVTVFNNVTIDKGNSPDTTITWNIGGNLSTPVNNWLTMVNGTLAYDR
                     TGDFFISTTTDFTIPATAGLTINTPSSVYISNSASSETLYLNGRMRILTGGGNVYIGP
                     PGNTGNNADIEYSGSGASLLEVQSGNLFVNGQIRRPLASTNGILAYRQTGGNVFIFGN
                     NTNQAKAKLEVLNDGSEFTMSGGNLTIVRGGGTTFGDLYLRPASGTMTGGTVIFTQIP
                     SAGPVIDSDQSYQLDANIPLNNLTVSGKTSGANRNASLALMISPLVVNGNLSMANSRS
                     VLASNNRNITIRGNLINNGTYNYGTNRTIFDGGVQIITGASVTDFYDMEVSALTSLTV
                     NGNFSVNHDLEIVSGNLVLGSGLLTLRGNLNNNGAYTDDNSTGGINLGGSAQQTMSGT
                     GSFARLVVDNNAGAKLNNDLALQHDLVLDRGVLDIGKYQLTLSQNSIIHGTGFSSSKM
                     IRSDGVASSRGLLKYFPTGAQTFTFPAGVAGKYTPALFTATASSTVGSVRINPVNEYH
                     PAILSPLNALGFYWQAESSGISGLNASLVFSYLTADVSGSEAAYVAARLLMPGGTWDK
                     AAPGAATDNVNEAANNISFYFTGSNNMNGDYTAGNDAAIPDEVPEYITNSNGNWSDAS
                     IWTPVGASPPCPAGGPKGYNVIIDHVVTADINFISVLNAVINGELRLVSPTYGHNLGN
                     VTGNGKIYVEGGNLPGGTYTEFTDCSGNGTIEYGGNGTYIIVSGVYSSVPNLIFSGTG
                     RRILPNSDLTVCKRLVIDGPILDNSVNNRKITVQGTFERYNSGAFRAGAGAYPAATVS
                     FSGSAPQTLGGATGDFSGTDRFWNVEINNPAGLTISDGSLIEAGNQLKLTNGVINTSA
                     AGRLVLLSASPSAVTPVGGSSVSYVSGPLTKQIINGDSFVFPLGKGPVMGHRFTLTSA
                     AGVTASFTAEFFSPNTTATSIAPPLEVTNTLEYWNVSSPSAASARIKIGWDPQSDLTP
                     LVTGNGLADMRVARYISGLWTEQPSTASGTNNNGEVSTTGTVSVSATPSDFTTASISG
                     TLARASFSSFTPICGSGGIPVSFISFTPISLTYILSYTINGVAQPDVTVTSLPFSLPA
                     NVPGVYRLTGFRFNSGSGTGVVDANTVTVYELPPASFAGTDQSLCGLSTTALSGNNPG
                     SFSGLWTVVSGAGGSFENSTLYNTVFNGVLGVSYTLRWTISNGPCSSRDEVVISFPVV
                     ASRPGDFTASESGVCPGGTGYVYTVPFVSGVTYNWSYSGTGHTINGSGNSVTVDFSMA
                     ATSGTLSVTATNACGTSPARSVNITVRTASFAYPDSPYCQNGADPLPVLNPGGVAGTF
                     SSTPGLVFINTATGQIDVSASKSGTYTVTNTADAAVCTGLSATATVTIAGQVWNGSVN
                     SSWDNPANWSCGFVPYPVTDVLIPDVSVDPVIASGLTGEAGNLVMDPGASLALTGGTM
                     RIYGSVSGSGSFNASSGTVEFRGSEPQLVVNGLFAGNTVMNMTANNPAGVTLGGPLNV
                     TGVILVQSGELASEGNLTLASDASRTALIDGSGAGTVTGDVTMQRYLPTKFGYRYFSS
                     PFIAATVSEFADDMNLASSFPLFYRYMESRTSSGWVAYNTGTALLNPMEGYSVNFGSV
                     DLPGTVDVTGVVNNGAVNVTLQNHNHQFTKGFNLAGNPYPSPVNWNASGWTKTNIDNA
                     LYFFRASSTDEYGGTYSSYVNGVSSDGIANNIIPSMQGFFVHVSDGTYPVTGTLGATN
                     SVRVNDQTHVFFKSAESGERFLIRLAAAFTDDTLSSDPAVVYFDNDAAPAFDPELDAL
                     KLFNTDLMVTNLYSVLPGGARLSVNALPPQADSIVWVPLGLITYRDGEVAFRMRDLEN
                     LPDNARIMFRDATAGKEIDLAGSEAYKVSLAAGDYNNRFALGILKSTTGAGVVYGDEI
                     LFSAYTSGQQVRATVGAIDGKEGLITIFDLGGRPVFERKVFEAGLHNLDVKLKQGVYL
                     VNYSTGTRKATIKLAIGL"
     gene            9071..9604
                     /locus_tag="PKK03_05580"
     CDS             9071..9604
                     /locus_tag="PKK03_05580"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HNX83903.1"
                     /translation="MISEKTYNTKSASGWLPLIMMVVLFGSVLPLMAQEPPPRPVIIT
                     ANPSQPLAFGAFTPGAAGGTITVPPDGSSRTTSGTVIPLNLGMMHTPAMFYIKANPGT
                     VISLLAGPPSPISGSNGGTLYLQLDDTYPASPLVTMVPYQQQTTVLVGGTLTVGTIGS
                     NPPGNYSGTINVTFVQE"
     gene            9625..13353
                     /locus_tag="PKK03_05585"
     CDS             9625..13353
                     /locus_tag="PKK03_05585"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNX83904.1"
                     /translation="MTERNAYKSIQFSDFMHVLSPGIRVIILIICLLPLTHRALAQGG
                     EQEYDEISVYIKIPYIGIREIDALIRGEEVYLPVRDLFNFLRIRNVVSDDFETVTGFF
                     LAAEDTYTISRESNQIKFDGKSYQLEPDALIRTETNLYLKSLYYGRVFGLDCHFSFRD
                     LTVNIDTKIELPVIREMRQEEMRRNITRLKGEIMADTTIGRSYPGFRFGMADWSVYAS
                     QQPGGKSDGRFNLALGSVIAGGEASASLTLYTGAAFSEKQQYYMWRHVNNDRSWMRQV
                     MLGKINTGATASLFNPVVGVQVTNTPTTFRRSFGTYSLTDRTEPGWTVELYVNNVLVD
                     YLKADASGFFTFEVPLVYGNTLVKLKYYGPWGEERTREQNITIPFNFMPHKEFEYTVS
                     AGFVEDSSWSRFSRADLRYGATKHITLGGGLEYLSSITSSPAMPFVNASVRITNNLLV
                     SGDYTHGVRAKGTMSYRMPSNIQLDLNYIWYERNQKAISYNYLDEIKASLSVPLRIKK
                     MGAYSRLTWHRISFPGSKYSTAEWMLAGALSRMSANITTYGAFSEYFDPNFSSQLSLG
                     FRLPANFVIMPQAQYNYTFKEFVSGKISLEKRLFNSGYFNLSLEHNFRNKITMGEVGL
                     RYDFSFAQMGFSVRQSNEQTSFVQYARGSLINDKKTSFLKADNRTNVGRGGVSILAFL
                     DLNANGVRDGNEPRADGLTIRSNSGRIEYSEKDSVIHIIGMEPYVKHFIELDESNFTD
                     VSWRLHKKTYAVVADPNMLRLIEIPVSVEGEATGTVMLEKDGLRSGLGRIIVNIYRSD
                     RTVKARVQTESDGYFSFFGLSPGSYYAKVDTAQLRKLDMTSEPDSIAFSIKSSREGDY
                     ISGLDFTVRKSEFIPAEVIMPDSAMVEDIPQIAGIVTGTDTLKIVPPKPGRTIAKDTS
                     YLVIHEVTRELVTISEDYYAVQFGAFRNKLYAEIMKKKVERALDKNVELFEEDGFWKV
                     RITGFEDREDLEKYIPIIHSQGITEIWVISNKAVRGEWITQTREDSLAMVRGTIEEAP
                     MPVVIRGTTVQLGAFSTIEETASMSDRLLAAAEKLVTIRNEGGFFKLQITGFTDTNEV
                     REFIPLLRKHGFDDITVLHESETGLVPVVPAAITPAAVQPAEPEIAEEPPVRDVQPEI
                     IIEQPEPVEEITPPPPPVPRFVLHAGSYYRKAEAERAKQRIEKRLKLPVEILEEWDSY
                     RVIITGFFTREQTYPFYPELAGLGFTDIFVYEKPLTER"
     gene            13370..14212
                     /gene="nfo"
                     /locus_tag="PKK03_05590"
     CDS             13370..14212
                     /gene="nfo"
                     /locus_tag="PKK03_05590"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HNX83905.1"
                     /translation="MKYVGAHISASGGVENAPLNAHEIGAKAFAFFTKNQKQWVAPPL
                     TSKSIGEFRKRCEEYGFKPFQILPHDSYLINLGSPDPDGLGRSRHSFTDEMKRCELLG
                     LDRLNFHPGSHLGKITEEECLNLIAESVNIALEKTSGVIAVIENTAGQGTNLGYRFEH
                     LRHIIDRVDDKSRVGVCIDTCHAYTSGYDISAPDGFRKVFDEFERIVGFGYLKGMHLN
                     ESKKAFGSRVDRHDSLGKGELGDDVFRLIMNDPRFDDMPLILETPEETLWAEEIMFLY
                     SLQT"
     gene            complement(14287..15366)
                     /locus_tag="PKK03_05595"
     CDS             complement(14287..15366)
                     /locus_tag="PKK03_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HNX83906.1"
                     /translation="MRNITTTMTHLLSPLRIRNIELRNRIAMSPMCQYSAKDGLSNDW
                     HVVHYGTRASGGAGLIIAEATAVWPEGRITPGDLGLWSDDHIPGLRRITDFIHSQGSV
                     AGIQLAHAGRKASCAVPWDGGKQLGLNNGGWNTVAPSAIPFSPGERAPVMADNVCLER
                     IIEGFRETARRALKAGFRVAEIHSAHGYLLHEFLSPLSNRRTDEYGGSFENRTRLLLE
                     VTTAIRSVWPDEYPLFVRISSTDWTDGGWTPEESVRLAPLLQKSGADLIDCSSGGNVH
                     NAKIPTGPGYQVPFAERVRKTGIMTAAVGMITETSQAEQIISEGKADLVLMGRELLRN
                     PYFPLRAAGERGEEINWPKQYLRAK"
     gene            complement(15396..15884)
                     /locus_tag="PKK03_05600"
     CDS             complement(15396..15884)
                     /locus_tag="PKK03_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83907.1"
                     /translation="MARSRYSGAFAALFLLFSICPAGAQNRFEVSAGAGLFDGGFIKA
                     RYGNRIEVGLSQDLVSQLHTTGLEVYFRIPRKYEPALPGPFYIMCGVSTTLFGKGYDT
                     FEQTFIYPRIGRSFLFRGKTGRAGLNADIGFAVHRHTNPPEGYLTEVIPISCSAGFFY
                     RF"
     gene            complement(15970..16854)
                     /locus_tag="PKK03_05605"
     CDS             complement(15970..16854)
                     /locus_tag="PKK03_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNX83908.1"
                     /translation="MPARKLTQTVRGQQAVDGAGVRLRRVLGYRTIRDFDPFLMLDGF
                     DSEDPRDYIKGFPWHPHRGIETVTYLIRGEIDHGDSLGNKGIIGDLQCQWMTAGSGII
                     HQEMPVASERMLGCQLWVNLPAKDKMTRPAYRDIRKENVEVVSEANATVRVLSGNYSG
                     KSGAVRGDYVGVQYLDVDLAPGSTWNYRGTPNDQTLFLYLIEGTLAADESLTGYEEKA
                     CAMLMTASSGNGNEYDEVRVKAGPAGARFLLIAGKPLKEPVAWGGPIVMNTQEELNLA
                     FSELQEKTFIKHDNPQEF"
     gene            17081..18028
                     /locus_tag="PKK03_05610"
     CDS             17081..18028
                     /locus_tag="PKK03_05610"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HNX83909.1"
                     /translation="MKKILLSGLVVLFCTSMQAQNGFNLKMNLEKNKVYRLMSVSSQT
                     VTQTINGVQQTTESKVTYAMSLKMLEATPGFMVTEVRFDSLVTITNTMGRIMTISSAN
                     EGDIKSEEMADVLSCIMNRLSRNALYVKIDYTGKPVEIVNARMLSDIVLKDTSTISVK
                     GPTAQAVRKQAVETVSEESLKSMIAMFTHFLPGGQVAKGEQWSITQQLNSGGMLLDII
                     STYRLNGSDGNLADITTESAIKAAENAAPIESGGATVTYGDLKGMSRGTMVIDVNSGM
                     RVAETSKTHISGNLGISGAGFSMQMPMDINSESKITLIQ"
     gene            18047..20416
                     /locus_tag="PKK03_05615"
     CDS             18047..20416
                     /locus_tag="PKK03_05615"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX83910.1"
                     /translation="MKGILVGLLLAASVAAQAQNISGKVCLENDRSPVPYATVGLVQL
                     PDSAMLTGVITLGDGGYLLENVKPGNYFIRVSYVGYRTNGRSVTVADGDGEVKVDTIF
                     LAETTASIDEIMVVGERLKGKEMVDRTTYAIPEVVARTATNGYDLLKKIPQVNVDYQN
                     NVTLNGSSNFIIQVDGRQRDKEYLARLLPSDIQSIEIISNPSGKYEGNIDGVISIILK
                     KEARYGINGNVGLNLKPFNKVTSQTTGSIDYSVGKITFYLTALNIYQALDISQTNESR
                     FTAIDSTISLYGEGGLAVTMTTINSGFDYYINDRNSLSLNVSYKPITQDIDLLGETFL
                     YKAENPLSTVITNTLNGYKSDESSASLFYKRNYRNPVQELTVENTYYNFRSDEDNSFD
                     NTRYPYNSTTVLDTYSRHEENLNTRNYYSAKVNYVHPLGMNAKLEAGYQLYYQQMSYD
                     FTVNDVESDNLFEYNELRNSAYGGVTYNMKKIGAQAVLRVENSHISADSVTRPDYTCI
                     LPSVNLQYKFSPSHNLKFTYNRRINRPGIYDMNPYFRTDQNYNISQGNPDLRPDYRDR
                     LQLTYTWNFGSNYFSPYIYNEYFSDRTGSEYLVTRSPVDNSLITFTKPYNLLSGYELG
                     GGVNATLWYINFNSRFYKGHINEYEGQTFTIPGRDYYSWSMTGAAYVSFGKNKKTTAF
                     AFLSYDGINMNARSKTYNHPFYGPGFQTQIKNHSMGVVWVLPLSTNVKLNRTETEAPE
                     FTSTNIIGFNASNFFQFSYSYKFNKGRSVKKLDRKVEVESDSKSGAIMK"
     gene            20543..20731
                     /locus_tag="PKK03_05620"
     CDS             20543..20731
                     /locus_tag="PKK03_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNX83911.1"
                     /translation="MAITKVWIEEGCIACGSCEQICPEVFRVTDQSRVQEGVNFSDYE
                     DGIKEAAECCPVEVIKYS"
     gene            complement(20744..21988)
                     /locus_tag="PKK03_05625"
     CDS             complement(20744..21988)
                     /locus_tag="PKK03_05625"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNX83912.1"
                     /translation="MKKRIIVIGGGFAGLQFIKNLKPGMFDVVLIDRLNHHQFQPLFY
                     QVATSQIEPTSISFPLRKIFQDRKDITVRMADVLAAKPQNKSVMTTAGAFEYDFLVIA
                     TGGKTNYYGNSELGKHTLSLKSTYEAIRIRNVILENFENILNSGENADEGLLNLVVVG
                     GGPTGVELSGAFAEIKRDILPKDFPGIDFSRLKIYLLEGGDHTLNTMSPLARRSSEKY
                     LTGLGVDLRTGIFVRNYDGSTLELSNGELLKSKNVIWTAGITGNIIDGLSPEVITRAG
                     RIKVDRTNRVTGYENIYAIGDIACMETPKYPKGHPQVANVAINQGKNLARNFTRMTKG
                     RKLAEYEYRDLGSMATVGRNKAIVDLPLVSFHGYLAWYFWMFLHLMLILSVRNKLIIF
                     INWAWNYITKNSSLRLILKVNS"
     gene            22095..22445
                     /locus_tag="PKK03_05630"
     CDS             22095..22445
                     /locus_tag="PKK03_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009434909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HNX83913.1"
                     /translation="MKVRRIYDPAEPEEGYRVLVDRLWPRGIAKEKAGWDEWMREVAP
                     GNELRKWYGHDPSRWEAFKEKYRQELSSRNDLLLRLRDLERAHGTLTLLFSSKELSLN
                     NAVALREFLMQQDR"
     gene            complement(22442..>23001)
                     /locus_tag="PKK03_05635"
     CDS             complement(22442..>23001)
                     /locus_tag="PKK03_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009236071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNX83914.1"
                     /translation="HNLQLSRATAWVKAYRWPIYFIISVSFWNLVGAGLFGFLINPPI
                     ALYYMQGLNTTPLHGHAALFGVFGMLAIGLMLFVLKGLTIKYRWKTGMLRIAFWTMNG
                     GLMLMLLLSLLPVGVLQTIASIKVGMWYARSADFMQGDTMETLRWLRVIGDTVFAVGS
                     VALALFVVGLKTGWSIDKTQKLYQD"
BASE COUNT         5901 a         5843 c         5717 g         5540 t
ORIGIN      
        1 acaacggcac agtgcggttc accaacctta attacccgat atacaatgcc tttcctccca
       61 ccacttcggg tgcgacatca ggagccgcca ctgtgtactt tgaaggagca tcagacaata
      121 tcctgacctg caacggggtg acagactttt acaacctaat tgtcaataag ggaactgatc
      181 agacatacgg tcttacggta agctcttccg cctaccctaa tttcagaatc tttggcgcca
      241 acgatcagga ggcagaatct gtttccggga atccggccat gaggaaggcg ctctggattt
      301 atgccggtac gcttgtactg aagggaaata tatttattcc ctcattgtct gaggggagcc
      361 tggccaatgc ccattattat ataccttcca acggtgccct gatatgtgac ggggttgacg
      421 tggttgtttt tgttactgca gacgattacc gggaagttaa taccgcttat ggcgtatctg
      481 cctccgataa cgtagcaatg ggaataaata ctgcagggac aggaagttct ctgtacgtgt
      541 tcggaaggct tggggtgaac aacggagttc tttctgcgaa ggaatcaggc gggatagtga
      601 catctgccac tgcttccggc cagataataa ttaatggcgg tgtggttgat gccaaacaat
      661 ttctgtcatc atccggttct gcttcttaca gccagtcggg cggtcttctg atcctcaggg
      721 gcaggttccg gagaacaccg gttgtctaca caaccataac cggtctgacc gatatgactg
      781 ccgcatctct gaacaccagc aggacaacgt cgggtatcag cgccttgcat ggttcattca
      841 atctggaaaa cacagacaac atctatacgg tttccggagg taccattcgc atttatgacg
      901 ttacaacaac tgccaccgcc gaagcatttg atgtcaaatc atccccggca aacatcaatg
      961 taacaggtgg tactcttgaa attattccca ccggcggatc cgggactgac gctacaagtt
     1021 attcaatcta ttccacggca cccctgcata acctgattat caacagggca agcggagccg
     1081 ctgtggtcag aatgagtacg ccactaaatg ttacagccag tctttcggtc atctcaggcg
     1141 ctcttaatgc aaacagcagc gatctttcgg ttggcggcaa tttcctgctt gaaacaggca
     1201 caacctatac caccggaacg aataccacca catttaacgg atcctcagcc cagctcttta
     1261 caatcaacca tgcatcaccg ctttctctca gttctttttc tgttacaaaa cccggcggag
     1321 taatccttgc ccttgccggt actcagaaca ctgtaaatgt ttcctcggcg ttaaacattg
     1381 ttgcgggcac cctcaatgac ggcgggaaaa tcctgaacgt ttccgggaac atttttaact
     1441 ccggccttgc gaccggtacg gggaaaattg tgctcaacgg cacaactgca caaaccatta
     1501 acggaggagg cacatttggc aatcttgagc ttgcaaacac gtcagccact cctgtttccc
     1561 tgcttaacag aacgactgtt aacggagacc tgaacttcgc tgttaacggt aacttcaaca
     1621 tcggcactta caatcttcac ctgaatgata cggcctcaat actaaatgcc ggaacagcca
     1681 ggttcatcca gaccgccgga aatgcaggtg acggaggtct ttcgcgaagc tactcatcaa
     1741 atgactcctt tattttcccg gtggggatga ccgggagata cacgccggcc acgattgggt
     1801 tcacggcctc gccggcagcc tacggttcag tttcggtaaa tccggttgat tatgagcatc
     1861 cggtcacaac tgtcaacggt cagagcctgt cttattattg gcgggttaag tcaaccgggt
     1921 tttcggggat tccgttattt tcagtcagcc atatatttgt ttacagtcag actgatgttg
     1981 ccggaacgga aagcagttat gtgcctgccc tttatgacaa cgtgtcacga agctggtata
     2041 taggtcgaac ctctgatatt aatataagca ccaataccat ttctgactgg gcaaccccga
     2101 ccaacagcac caacttcctt gatggtgatt acactgcagg aagtgccggc agttttggcc
     2161 tgcccaggat atactatagt cgtcagaacg gtctctgggg ttcaacttca acatggtctc
     2221 ttaccggaca cacagtgaca aatccgcctt caactgctcc gggtgcgaat gatattgtaa
     2281 tcatcggcgg gaatgattca atatggctgg caacagaaac gccacctctt ccaccggcac
     2341 ccggctatca gcccgcatca tattatcagc gtaacaaagc tgtcgtcaac tgtgccacac
     2401 ttcagataga ggcagcctca gtccttgata ttcagaacaa cccgggcagc acttttgcat
     2461 cagttttaag tcaccctaac ggcaacggaa agatcaggat aacaacccgc gacgcttcga
     2521 atttcgaaaa ctctgaacct tttgtctatc cttcaggtga cttttcagag tttagcatta
     2581 acgatggtat ttcagagttt tatactatta atccccgggc tggtgcatat tatattttgc
     2641 cttcgaatgc cacggaatac gggtcagtaa tcatgacacc cctgaaaggg tccaacataa
     2701 ttatgccaaa tcttccgcaa gttaccgtta acggagacct gatatgcaat ggttctgatg
     2761 ctgatgcctg gctggccatg tcatggtctg gtacagatta tggaacaatc ggtgaaaaga
     2821 cggtaaatgt aatgggcaac ctgattgtcg cagggggctc gttcggtttt atatacaacg
     2881 gaacaacact gcagagaata aatattgatg gtaatgttta tgttgccccg ggcgcaggga
     2941 tagatgtttg gaactcctcc acgaataata tcatgtcgat cggaggctct gtcttcaata
     3001 acagcaataa tacaactgca ccttccggtt cacccagtgt tatgcggttt atcaattcag
     3061 gaaacaggtg tgacctgatc ttcaccggca acaggtcaac cattgtcaca aacaaccctg
     3121 ccctgagcac cacacctgta acggtattca acaatgttac catagacaag gggaattcac
     3181 ctgatacaac cattacctgg aacattggcg gaaacctttc cacccctgtc aacaactggc
     3241 tgacaatggt taacggaact ctggcatatg accgcaccgg agactttttc atatccacaa
     3301 ccaccgactt cacgattccc gccacggcag gacttaccat caacacacca tcaagtgttt
     3361 atatttcaaa ttcagcaagt tctgaaacgc tgtacctcaa tggcaggatg agaattctga
     3421 caggtggcgg aaatgtctac atcgggcccc cgggaaacac cggaaacaat gctgacattg
     3481 aatactctgg aagcggtgct tcacttctcg aggtacagag cggaaacctg tttgtcaacg
     3541 gccagatcag aagaccgttg gcatcaacca acggtatcct ggcatacagg cagaccggag
     3601 gcaatgtctt cattttcgga aataatacaa atcaggcaaa agccaaactg gaagttctga
     3661 atgatggcag tgaatttact atgtcaggag gcaatcttac aattgtccgc ggcggaggaa
     3721 caaccttcgg ggatctgtac ctgagaccgg catccggaac gatgacagga ggaaccgtga
     3781 tctttaccca gataccgtct gcaggcccgg taatagattc agatcagtct taccagcttg
     3841 atgccaatat tcctctcaat aacctgacag tatcaggtaa aacgtccggt gcaaaccgca
     3901 atgcaagcct tgcccttatg atcagcccgc tggttgtgaa cggcaacctg tccatggcca
     3961 acagcaggag cgttctggca tcaaacaacc ggaacataac aatcaggggc aaccttatca
     4021 ataacgggac ctacaattac ggaacaaacc ggacaatatt tgacggtggg gtgcagatca
     4081 tcacgggcgc atccgtaacc gacttttatg atatggaggt ttctgccctg acctctctca
     4141 cggttaatgg caacttttca gtaaatcatg atcttgagat agtatccggt aacctggttc
     4201 ttggatcggg actgctgaca ttaaggggaa acctcaataa caacggtgct tataccgatg
     4261 ataattcgac aggcggaatt aatcttggcg gcagtgcaca gcaaaccatg tcaggcacag
     4321 gctcatttgc caggctggtg gttgacaata atgccggagc aaaactgaac aacgatttag
     4381 cccttcagca tgacctggtg ctggacaggg gagtgcttga catcggcaaa taccagctca
     4441 ctctcagcca gaacagcata attcatggta ccggtttctc ctcttcgaaa atgatcaggt
     4501 ctgacggcgt ggccagcagc aggggtctgc ttaaatattt cccgacagga gctcagactt
     4561 ttaccttccc tgcaggtgtg gccggaaaat atactcccgc cctcttcacg gcaacggcaa
     4621 gctcaacggt agggtcggtc cggataaacc cggtcaatga ataccatccg gccatcctga
     4681 gtccccttaa tgcccttggg ttttactggc aggcagagag ttcgggtatt tccggactta
     4741 acgcgagcct ggttttcagt tacctgacgg ctgacgtgtc agggtccgag gcagcctatg
     4801 tcgctgcacg ccttttaatg ccgggaggca catgggacaa agctgcaccc ggagccgcaa
     4861 ccgacaatgt gaacgaagcc gcaaataata tttccttcta ttttaccggc agcaacaata
     4921 tgaacggcga ctacactgcc ggaaatgatg cagccatacc tgacgaagtt ccggagtaca
     4981 taacaaacag caacggcaac tggtcggacg cctcaatatg gacgccggtg ggtgcatctc
     5041 ctccctgccc ggcaggcgga ccgaaggggt acaatgtaat aattgatcat gtggtcacag
     5101 ccgatataaa cttcatttcg gttcttaacg ctgtgattaa cggagagctg cggctggttt
     5161 cacctaccta cggacacaat cttggcaatg tgacaggcaa tggcaagatc tatgttgaag
     5221 gaggaaacct tccgggaggt acttataccg aatttactga ctgttcgggg aacggaacaa
     5281 tcgaatacgg aggaaacggg acatatatca ttgtttcggg cgtatattca tcggttccga
     5341 acctgatctt ctccgggaca ggacgaagga tcctgcccaa ttctgacctg accgtatgca
     5401 agcggcttgt cattgacggc cccatccttg ataacagtgt caacaacagg aaaataaccg
     5461 ttcagggtac atttgagagg tacaattccg gagcattcag agccggagcc ggggcttatc
     5521 ctgcagcaac cgtatcattt tcagggtctg ccccccagac cctgggtggc gccaccggtg
     5581 atttcagcgg tacagacaga ttctggaacg ttgagataaa caatccggcc ggcctgacaa
     5641 tcagtgacgg cagcctgatt gaggcaggca accagctgaa gctcacgaac ggagtcatca
     5701 acacatctgc agccggaaga cttgtgcttc ttagtgcctc tccatccgca gtgacgcctg
     5761 ttggcggcag ttccgtgtct tatgtgagcg gtcccctgac aaaacaaata atcaacggcg
     5821 attcctttgt atttcctctt ggaaaaggcc cggtaatggg tcacaggttt acactgactt
     5881 ctgcagcggg ggttaccgca tcctttacgg ctgagttttt ctcgccgaat accacagcaa
     5941 catccatcgc cccgccgctg gaagttacaa acacccttga atactggaac gtttcatcac
     6001 cttctgctgc ttcggccagg attaagatcg gctgggatcc gcagagcgac ctcactccgc
     6061 tggtcaccgg gaacgggctg gcagatatga gagtggcaag gtatatctcc ggtctgtgga
     6121 cagaacagcc atcaacagct tccggaacaa ataacaacgg ggaggtgtct accaccggca
     6181 ctgtatcagt ttctgccacc ccatctgatt ttacaacagc aagtatttcc ggaacacttg
     6241 cgagagcttc attctcttcg tttactccca tctgcggctc aggcggcata ccggtcagct
     6301 tcatctcatt taccccgata agtctcacat atatattaag ttatacaatc aatggcgtgg
     6361 cgcagccaga tgtgaccgtc acatccctgc cgttctcttt accggcaaat gtaccggggg
     6421 tatatcgcct taccggattc aggttcaaca gcggatccgg caccggtgtg gttgatgcaa
     6481 acaccgtgac cgtctatgag cttcctcccg cctcatttgc aggtactgac cagtcgctct
     6541 gcggcctttc caccactgcc ctttccggta ataatcccgg atccttttca gggctctgga
     6601 ctgttgtcag cggtgccggc ggttcatttg aaaacagcac tctttacaac accgtattta
     6661 acggtgtgct gggagtaagt tacaccctcc ggtggacaat aagcaacgga ccctgttcct
     6721 cccgggatga ggtggtgata tctttcccgg ttgttgcttc aaggcccgga gactttactg
     6781 cctctgaatc cggagtatgc cctggcggga caggatatgt ctatactgtt ccgttcgtct
     6841 ccggggttac ttacaactgg tcttacagcg gaacgggcca tacaataaat ggcagcggta
     6901 actccgtcac ggtagatttc agtatggctg ccaccagcgg caccctgagc gtgacggcaa
     6961 caaacgcctg cggcaccagt ccggcaagat cagtgaatat cactgtgcgc acagcttcat
     7021 ttgcctatcc ggattctcct tactgccaga acggcgcgga tccgttgccg gtcctcaatc
     7081 cgggaggagt tgcgggaaca ttctcatcaa ccccggggct cgtcttcatt aacactgcca
     7141 ccggccagat agatgtatct gcaagcaaat cgggaacata tactgttacc aatacggcag
     7201 atgccgctgt atgcacgggg ttgtcagcaa cggcaaccgt aacaattgca ggccaggtat
     7261 ggaacggttc cgtaaacagt tcatgggata atccggcaaa ctggtcctgt ggtttcgttc
     7321 cgtaccctgt gaccgatgtt ctgatccctg atgtgtccgt tgacccggtc attgcttccg
     7381 gattaaccgg tgaagcaggt aatctcgtga tggatcccgg agcatccctt gcccttaccg
     7441 gcggaacgat gaggatttac ggatcagtat cgggcagcgg ttcattcaat gcttcctccg
     7501 gtactgttga gtttcgcgga agcgaacccc agttggtggt aaacggcctg ttcgccggaa
     7561 atacagtaat gaacatgact gcaaataatc ctgccggtgt cacacttgga ggtcctctca
     7621 atgtaacggg cgtgatactt gttcagagcg gggagctggc ttcggaagga aatctcaccc
     7681 tggcatctga tgcgtcgcgc actgctctta tcgacggttc cggcgcaggg actgtgacag
     7741 gcgatgtcac tatgcagagg taccttccca caaaattcgg ttacaggtat ttcagctctc
     7801 ctttcatcgc cgccaccgta agtgaatttg ccgatgacat gaacctcgcc tcttccttcc
     7861 ctctgttcta ccggtacatg gagagcagaa catcatcagg gtgggtggca tacaacacag
     7921 gtacagcgct tcttaacccg atggagggtt attcagtcaa tttcggatct gttgacctgc
     7981 cgggtactgt agatgtgacc ggtgttgtca acaacggagc tgtcaatgtt accctgcaga
     8041 atcataacca ccagttcaca aagggattta accttgccgg aaatccgtat ccgtctccgg
     8101 tcaactggaa tgcatcaggg tggacaaaga ccaatattga caacgccctt tacttcttca
     8161 gggcaagctc taccgatgag tacggcggta cctacagcag ctatgtcaac ggcgtctcaa
     8221 gcgacgggat tgcaaacaac atcattccct ctatgcaggg tttctttgtg catgtctctg
     8281 acggcactta cccggtgacc ggaacgcttg gcgcaacaaa cagcgtcagg gtcaatgacc
     8341 agactcatgt attcttcaag tcggctgaat ccggggagcg ttttctcatc aggctggcag
     8401 ctgccttcac cgatgacacc ctttcttctg acccggcggt agtttacttt gacaatgatg
     8461 ccgcacctgc ctttgacccg gaacttgacg cccttaagct cttcaacacg gatttgatgg
     8521 tgaccaacct gtattcggtt cttcccggtg gagcacgtct gtctgtcaat gccctgccac
     8581 cacaggcaga cagtatcgtg tgggttcccc tgggactgat cacttaccgt gatggagaag
     8641 ttgccttcag gatgcgcgac ctggaaaatc tgcccgacaa tgcccgcatt atgttccgtg
     8701 atgcaacagc cgggaaggaa attgacctcg caggttcaga agcatataag gtgagcctcg
     8761 ctgcaggtga ttacaataac aggtttgcac tgggaatact taagagcaca accggcgccg
     8821 gcgtggtata tggcgatgag atcctgttca gcgcatacac ttcagggcag caggttcggg
     8881 caactgtagg agcaattgac ggaaaagagg gtttaataac gattttcgat cttggcggca
     8941 gacctgtctt cgaaaggaag gtgtttgaag ccggcctgca caatcttgat gtaaaactga
     9001 agcagggcgt ttatctggtg aattacagca ccggaacacg taaggcaacg attaaactgg
     9061 caatcggatt atgatcagcg aaaaaacata caacaccaaa tctgcatccg ggtggctgcc
     9121 attgatcatg atggttgtcc ttttcggaag cgttcttccc ctgatggccc aggagccgcc
     9181 tcccaggccg gttatcataa cggccaatcc atcgcagcca cttgcgtttg gcgcttttac
     9241 tccgggagca gcagggggga ccatcacagt gccgccggat ggttcttcaa gaacaacctc
     9301 cggcacggta attcccctga atctcggcat gatgcatacg cccgcaatgt tttatataaa
     9361 ggcaaatccg ggaacagtaa tatcgcttct tgccgggcct ccgtcaccca tttcgggaag
     9421 taacggcggc accctctacc tccagctcga tgatacctat ccggcttcgc cgttggtgac
     9481 tatggttcca taccagcaac agacaacggt tcttgtggga ggaaccctta cagtgggcac
     9541 tatcggctcc aacccgcccg gtaactatag tggaacaatc aacgtcacct tcgtgcagga
     9601 gtgatgcgag aaaaatatta attgatgaca gaaagaaatg catataaatc catacagttt
     9661 tcggacttta tgcatgttct ttctcccgga ataagggtta ttatattgat aatatgcctg
     9721 ttacctctga cacacagagc gcttgcccag ggtggggagc aggaatacga tgagatttca
     9781 gtctatatta agatcccata tatagggatt cgcgaaatag acgccctgat caggggagag
     9841 gaggtttatc tgccggtcag ggacctgttt aatttcctcc gtatcagaaa tgtagtctcc
     9901 gatgactttg agacagtcac cgggtttttt ctggcagctg aagatactta caccatcagt
     9961 cgtgaaagca accagattaa atttgacggt aaaagttatc agcttgaacc ggatgccctt
    10021 atcagaacag agaccaatct ttacctgaag tcactttatt atggccgggt attcgggctc
    10081 gattgccatt tcagtttcag ggatctgaca gtcaacattg acaccaagat tgagcttccg
    10141 gtgatccgtg agatgcgcca ggaggagatg aggagaaata ttacccggct taagggagaa
    10201 attatggcag acaccacaat tggccgaagc tatcccggat tccgttttgg catggctgac
    10261 tggtcagtct atgccagtca gcagcccggc ggcaagtcag acggccggtt taatcttgcc
    10321 ctcggttcgg ttattgcggg cggtgaagcc tccgcttccc ttactttgta taccggagcc
    10381 gcgttcagtg aaaagcaaca gtactacatg tggaggcatg tcaataatga caggagctgg
    10441 atgcggcagg taatgctcgg aaagatcaat accggcgcaa ccgcctccct gttcaaccct
    10501 gttgtcggag tgcaggttac aaatacaccc accaccttcc gtcgttcttt cggaacgtac
    10561 tctctgactg accggaccga accggggtgg acggtggaac tttatgtcaa taatgtgctt
    10621 gttgattatc tgaaggccga tgcttccgga tttttcactt ttgaagttcc gctggtttac
    10681 ggaaacaccc tcgttaaact gaagtattac gggccatggg gtgaagagag aaccagggaa
    10741 cagaatatca ccatcccgtt caatttcatg ccgcacaagg agtttgaata tactgtcagc
    10801 gccggatttg tggaagactc atcatggagc cgtttctcca gggctgatct gcgttatggc
    10861 gccaccaaac acatcaccct gggcggagga ctcgaatatc tctcatcgat aacttcatct
    10921 ccggcgatgc cttttgtgaa tgcttctgta aggattacca ataacctcct tgtttccggc
    10981 gactatactc acggcgtcag ggcaaaggga accatgtcat accgcatgcc gtcaaacatc
    11041 cagctggacc tgaattacat ctggtatgaa agaaatcaga aagccatttc atataattac
    11101 cttgatgaaa taaaagcctc tctctcggtc ccgttaagaa taaagaagat gggagcctat
    11161 tccaggctta cctggcacag gatcagtttt ccgggctcaa aatattcgac tgctgaatgg
    11221 atgctcgccg gtgccctgtc gaggatgagt gcgaatatca caacctacgg agcattctcg
    11281 gaatactttg atcccaattt ctccagccag ctttcactgg gtttcagatt gcctgcaaat
    11341 tttgtgatta tgcctcaggc tcagtataat tacaccttca aggaattcgt ctccggaaag
    11401 atcagcctgg aaaagaggct tttcaacagc gggtatttca acctttccct tgaacacaac
    11461 ttccgtaata aaatcactat gggagaggtg ggccttcgct atgatttctc gttcgcccag
    11521 atgggcttct cggtgcgcca gagcaatgaa cagacatcat ttgtccagta tgcgcgagga
    11581 agcctgataa atgacaaaaa gacctcattc ctgaaggcag acaacaggac aaatgtcggc
    11641 agaggcgggg tatcaatcct tgcatttctc gacctgaacg ccaatggcgt ccgtgatggc
    11701 aatgaaccaa gggctgatgg gcttacaata agatccaaca gcggaagaat cgaatacagc
    11761 gaaaaagact cggtaattca cataataggg atggagccct atgtgaagca cttcattgag
    11821 cttgatgaaa gcaacttcac cgatgtgtca tggaggctgc acaagaaaac atatgccgtg
    11881 gttgccgatc caaatatgct caggcttatt gaaataccgg tcagcgttga aggagaggcc
    11941 accggaacgg ttatgcttga aaaagacggg ttaaggtcgg gtctcggaag aatcatcgta
    12001 aatatttaca gaagtgaccg cactgtcaaa gcaagggtac agaccgaatc agatggctac
    12061 ttcagctttt ttggactttc tcccggatct tattacgcca aagttgacac cgcccagctc
    12121 aggaagcttg acatgacctc agagcctgac tcaatagcat tcagcattaa gagcagccgt
    12181 gagggagact acataagcgg gctcgatttc acggttcgga agagcgaatt cattcctgca
    12241 gaggtcatca tgcctgattc tgcaatggtt gaggatatcc cgcagatcgc gggcattgta
    12301 accggcacgg ataccctgaa gattgttcct ccaaagccgg gcagaaccat agccaaagac
    12361 acgtcatacc tggttatcca cgaggtgacg cgtgagttgg tgacaatctc cgaggactat
    12421 tatgcggttc agttcggtgc attcaggaac aagctctatg ccgagatcat gaaaaagaag
    12481 gtagaaaggg ctcttgacaa gaatgttgaa ctgttcgagg aagacggctt ctggaaggtc
    12541 cgcataaccg gttttgaaga ccgtgaggat cttgaaaagt acataccgat tattcattct
    12601 cagggcatca ccgagatatg ggtcatctcc aacaaggccg tccggggcga atggataaca
    12661 cagaccaggg aggactccct tgcgatggtc aggggaacta ttgaggaagc tcccatgcct
    12721 gtggtaataa gaggcaccac ggtgcagctc ggggccttca gcaccatcga agaaacggca
    12781 tcgatgagcg acaggttgct tgctgctgcc gagaaacttg tgacaatacg aaatgagggc
    12841 ggcttcttca agcttcagat taccggcttc accgatacca acgaggtcag ggagtttatt
    12901 ccgttgctgc gtaagcatgg attcgatgac ataacggtgc ttcacgaatc cgaaaccggt
    12961 cttgttccgg tggttcctgc cgccatcacc cctgctgccg tgcaacctgc tgagccggaa
    13021 attgcggaag agcctcccgt cagggatgta cagcctgaga tcataattga acagcctgag
    13081 cctgtcgaag agataactcc gccaccgcca cctgtgccaa gattcgttct ccatgccgga
    13141 agctactaca ggaaagcgga ggccgaaagg gctaaacagc gcatagaaaa gcgcctgaag
    13201 cttccggttg agatacttga ggagtgggac agctacaggg tgatcattac cggtttcttt
    13261 accagggaac agacatatcc attctatcct gaacttgcag gattgggatt cactgatatc
    13321 tttgtctatg aaaagccgct gactgaacgg taggcaaata tcagatccca tgaaatacgt
    13381 cggagcacac ataagcgcat cgggaggggt ggagaatgca cctctgaatg cacacgaaat
    13441 aggagcgaag gctttcgctt tctttaccaa gaaccagaaa caatgggtgg cgccgcccct
    13501 gacctctaaa agcatcgggg agtttcgcaa aagatgcgaa gagtatggct ttaaaccctt
    13561 tcagatactg ccccatgaca gttacctgat aaaccttggc agtcctgatc cggatggcct
    13621 gggccgttca cgccactcct tcaccgatga gatgaaacgg tgcgagctgc tcggcctcga
    13681 caggcttaat tttcacccgg ggagccacct gggaaagata accgaggaag aatgcctcaa
    13741 ccttattgcg gaatcggtca atattgccct tgaaaagaca tcaggtgtca ttgccgtcat
    13801 tgaaaatacc gccggacagg gaacaaatct gggttacagg ttcgaacatc tcagacatat
    13861 tattgacagg gtcgatgata agagcagggt aggggtgtgc attgacacat gccacgcata
    13921 tacctcaggc tatgatatct ctgccccgga tggcttcagg aaggtttttg acgagtttga
    13981 gcggatcgtg ggtttcggtt acctgaaggg aatgcacctg aatgaatcaa aaaaggcgtt
    14041 cgggtcacgg gttgacaggc atgacagcct gggcaagggg gaactgggtg atgatgtctt
    14101 caggctgata atgaatgatc cccggtttga tgacatgccg ctgatccttg agacacccga
    14161 agagacgctc tgggctgaag agatcatgtt cctctattca cttcagacat agattacccg
    14221 gcccatttgc cgcatgacca tgtgctctgc gggcaatccg gctatggaaa tggtattcag
    14281 gctgatctat ttagctctca ggtattgttt gggccagttt atctcttcgc cccgttcccc
    14341 ggctgcccgc agggggaagt aaggattgcg cagcagttct cttcccataa ggacgaggtc
    14401 ggctttccct tctgaaatta tctgttctgc ctggctggtt tcagttatca tgccgacagc
    14461 cgctgtcata attccggtct tcctgaccct ctctgcgaac gggacctggt aacctggtcc
    14521 tgtgggtatc ttcgcattat ggacgtttcc gcccgaggag cagtcaatca ggtcagcgcc
    14581 ggatttctgc agcaacggtg ccagcctgac actctcttcc ggcgtccagc ctccgtcagt
    14641 ccaatctgtt gaggagatcc ttacaaagag ggggtattca tcgggccata ctgatcttat
    14701 ggctgtggtc acctcaagga gcagcctggt gcggttttca aatgacccgc cgtattcatc
    14761 cgtccgcctg ttgctgagag gcgaaaggaa ctcatggagg aggtatccgt gggcgctgtg
    14821 aatctctgcc acccggaatc cggctttcag agcacgcctg gcagtttccc ggaaaccctc
    14881 aatgattctt tcaaggcaaa cattgtcagc catcaccgga gccctttcgc ccgggctgaa
    14941 gggtatggcg gacggggcta ccgtattcca tcctccgttg ttgaggccga gctgttttcc
    15001 gccatcccat ggtaccgcac aggaggcttt tctgccggca tgggccagct gtatacctgc
    15061 cacggatccc tggctgtgaa taaaatctgt gatcctccgc aaacccggta tgtggtcatc
    15121 gctccacagt cccaggtctc ccggtgttat ccttccttcc ggccacacgg ccgtggcttc
    15181 tgcgataatg agcccggcgc ccccggaggc cctggtcccg taatgcacaa catgccagtc
    15241 atttgataaa ccgtccttcg ccgaatactg gcacattggt gacatggcga tgcggttcct
    15301 gagttcaatg tttctgattc tgagaggtga aagaaggtgc gtcatggttg tggtaatatt
    15361 tctcattaca acaggcaagt cccgaattat tgttttcaga accggtaaaa gaatcctgcg
    15421 ctgcaggaga tgggaataac ttccgtcaga tagccctcgg gcggattggt atgacggtgg
    15481 actgcaaatc cgatatctgc attaagtccg gccctgccgg ttttgcctct gaagagaaaa
    15541 gatcttccga tcctgggata gatgaatgtc tgttcgaagg tgtcataacc ctttccgaac
    15601 agtgtggtgc tcactccgca catgatgtaa aacggtccgg gcagggccgg ctcatacttt
    15661 cgcggtatgc ggaaataaac ctccagtccg gttgtatgta gctggcttac aagatcctga
    15721 ctcaggccaa cctcaatcct gttcccatat ctggctttaa tgaaaccacc atcaaacagt
    15781 cctgctccgg cactgacctc gaacctgttc tgtgccccgg ccggacatat tgaaaaaaga
    15841 agaaatagtg cagcaaatgc acctgagtat cttgaccgcg ccattactga tttaagttaa
    15901 agtccctgaa tgtactgaaa ttaaatttga aataccattg aagcggagcg ggtaactggt
    15961 gtcattcatt cagaattcct gtggattgtc gtgcttgatg aatgtctttt cctgcagctc
    16021 gctgaaagcc aggttcagtt cctcctgggt attcatgacg ataggtcctc cccaggcaac
    16081 cggttcctta agcggctttc cggctatcag aaggaacctt gcaccggcgg gcccggcttt
    16141 tacccttacc tcgtcatatt catttccgtt cccggatgat gcagtcatca gcatcgcaca
    16201 tgccttctcc tcataccctg tcagtgactc atctgcagcc agcgtaccct caataaggta
    16261 taggaacagt gtctgatcat tcggtgttcc gcggtaattc caggtgctgc cgggcgccag
    16321 gtcaacatcc aggtactgta cacctacgta atcacccctg acagcacctg atttgccact
    16381 gtagtttccg gagaggaccc ttacggtggc atttgcttcg ctcaccacct ccacgttttc
    16441 ctttcgtata tcgcggtatg cgggccgggt catcttgtcc ttcgcaggca ggttgaccca
    16501 gagctgacag ccgagcatcc tctccgatgc aacaggcatc tcctggtgga tgataccaga
    16561 tccggctgtc atccactggc actgcagatc accgattata cccttgttgc caaggctgtc
    16621 gccgtgatca atctcccccc tgataagata ggtgaccgtc tctatgccac ggtgcgggtg
    16681 ccacgggaag ccttttatat agtcacgggg atcctccgag tcgaatccgt caagcatcag
    16741 gaaaggatca aaatccctga ttgtcctgta gcccaggacc ctgcgcaaac ggacgccggc
    16801 tccgtccaca gcctgctggc ccctgactgt ctgggtcaat tttcttgctg gcatacaatt
    16861 aaattcagat tgattaccat gtaagtatac gaaaaataaa gtgacccgca agtgcctgtt
    16921 tccttttacc cgtgccgttt tcatcacccc tgcccggcag acaggaccgg ataaaaatgc
    16981 cctttacctg ccaaaaacat ccattcaccg actatcgatg gtctgagagc ctcacccagt
    17041 ataatttcgc tgagaaatga aaaaacctaa tcgcggtatc atgaaaaaaa ttctgttgtc
    17101 aggtcttgtt gttcttttct gcacttcaat gcaggcacag aacggcttca accttaaaat
    17161 gaaccttgag aagaacaagg tttaccggct catgtcagtc tcgtcgcaga ctgttacgca
    17221 gacaatcaac ggtgttcagc agacaaccga atcaaaggtc acctatgcga tgtctctgaa
    17281 aatgcttgaa gcgactcccg gctttatggt taccgaagta cgttttgact cacttgtcac
    17341 catcaccaat accatgggca ggatcatgac gatcagttca gccaatgaag gagatattaa
    17401 atcagaggaa atggctgacg tcctctcatg cattatgaac cggctgagca ggaatgccct
    17461 gtatgtcaag attgattaca cgggcaaacc ggttgagata gtaaatgcca ggatgctctc
    17521 tgacattgtt ctgaaggata cgagcaccat ttccgtaaag ggccccacag cccaggcagt
    17581 gcggaaacag gctgtcgaaa cggtcagtga ggaaagtctt aaaagcatga tcgcaatgtt
    17641 cactcatttt ctgcccggcg ggcaggttgc aaagggtgaa cagtggagta tcacacagca
    17701 gctgaactcc gggggaatgt tgcttgacat aatatcaaca tatcgcctta acggatcaga
    17761 tggcaacctg gcagatatca ccacagagtc agccattaag gctgcggaga atgcagcccc
    17821 gatagaatcg ggtggcgcaa cggtcaccta tggcgatctg aagggcatga gccgtggaac
    17881 catggtgatt gacgtcaact caggcatgag agtggcggag accagcaaaa cccatatctc
    17941 ggggaatctg ggaatcagcg gtgccggatt cagcatgcag atgccaatgg atataaacag
    18001 tgaatctaaa atcacgctaa tacaataaat caattcggga cagaccatga aaggaatttt
    18061 agttggattg cttctggcgg cctctgttgc agcacaggct cagaacataa gcggtaaagt
    18121 atgcctggag aatgacagat ctcctgtgcc atacgctacc gtgggccttg ttcagcttcc
    18181 agattcagca atgcttacag gagtaattac ccttggcgac ggcgggtatc tccttgaaaa
    18241 tgtaaaaccc ggtaactact tcataagggt aagttacgtg gggtacagga caaacggcag
    18301 aagcgtcact gttgccgatg gcgatggtga ggtaaaagtt gataccatct tcctggctga
    18361 aaccaccgcc agcattgatg agatcatggt ggttggagag cgcctcaagg ggaaggaaat
    18421 ggttgacaga accacctatg ccatccccga agtggtggcc cggaccgcca ccaacggata
    18481 tgacctgctg aagaagatac cgcaggttaa tgtcgattat cagaacaatg tgacactgaa
    18541 cggcagcagc aactttatca tccaggtaga tggacggcag cgcgacaagg aatacctggc
    18601 aaggctcctc ccctctgaca ttcagtcgat agagatcatc agcaatccgt caggcaaata
    18661 tgaaggcaat attgacggtg tgatcagcat catactgaag aaggaggcca ggtacggtat
    18721 caacggaaat gtgggtctca acctgaagcc attcaacaag gttacaagtc agacaacggg
    18781 aagcattgac tattccgtcg gaaagataac cttctacctg actgccctca acatttacca
    18841 ggctcttgat atcagccaga caaacgagag ccggtttacg gcaatcgact caacaatcag
    18901 cctctacgga gaaggaggac tggcggttac aatgacaaca atcaacagcg ggtttgatta
    18961 ttacataaat gacaggaaca gcctcagcct gaatgtcagt tacaaaccga taacacagga
    19021 catagacctg ctcggcgaaa cattccttta taaagccgaa aatcctctca gcaccgtgat
    19081 aaccaataca cttaacggct ataaaagcga cgaatcatct gcctcactgt tctacaagcg
    19141 gaactacagg aaccctgtac aggaacttac ggtggagaat acttattaca atttcaggtc
    19201 agatgaggat aacagctttg acaacacccg ttacccctat aattcaacaa cggtgcttga
    19261 cacatattca aggcatgaag agaacctgaa taccaggaat tattattccg ccaaggttaa
    19321 ttatgtccat ccgctgggaa tgaatgcaaa gctcgaagcg ggttaccagc tgtactacca
    19381 gcagatgagt tatgatttca cggtgaacga tgtcgaatcc gacaacctgt ttgaatacaa
    19441 cgaactcagg aattcagcat acgggggagt cacctataat atgaagaaga tcggggcaca
    19501 ggcagtgctc agggtggaaa acagtcacat aagcgccgat tcggttacca ggcctgatta
    19561 tacctgtatc cttccatcgg tcaaccttca gtataagttt tcaccgtctc ataacctgaa
    19621 attcacctac aacagaagga taaacaggcc ggggatctat gacatgaatc cttatttcag
    19681 gacagatcag aattacaata tttcgcaggg taacccggac ctccggcccg attaccgtga
    19741 caggctccag ctaacctaca cctggaattt cggcagcaat tacttctcgc catacatcta
    19801 caatgagtat ttctctgacc ggacggggag tgagtatctg gtaacccggt cgccggtaga
    19861 caattccctg atcaccttca ccaagccata taaccttctc agcgggtatg aactcggagg
    19921 cggggtaaat gcaacgctct ggtatataaa cttcaattcg agattttaca agggtcatat
    19981 taatgagtac gaaggtcaga cttttactat accgggcagg gattattatt cctggagcat
    20041 gacgggggcg gcatatgtca gttttggcaa gaacaagaag actactgcat ttgctttcct
    20101 cagctatgac ggcataaata tgaatgcccg ttcaaagaca tacaatcatc cgttctacgg
    20161 gccgggattc cagactcaga taaagaacca cagcatgggt gtggtatggg tcctgccttt
    20221 gtccacgaat gtgaagctga acaggacaga gaccgaggcg cctgaattta cgtcgaccaa
    20281 catcatcgga ttcaatgcct ccaatttctt ccagttctcc tactcataca agttcaacaa
    20341 ggggaggagt gtgaagaagc ttgaccgcaa ggttgaggtt gagagcgaca gcaagagcgg
    20401 ggctatcatg aagtgacaga agaagcaaaa tatcagttga acggcagggc cgggtgctgc
    20461 atagaacccg gctctgcctt tatttgaacc aaatgcatta tcttccgttt ctgttttatc
    20521 agttacaacc attaatagcg atatggcaat tacgaaagta tggattgaag agggctgcat
    20581 cgcctgcggc tcatgcgaac agatctgtcc tgaggttttc agggtcacag atcagtcgag
    20641 agtacaggaa ggagttaatt tctcagatta cgaggatggc ataaaagagg cagctgaatg
    20701 ttgcccggtt gaggtaatca agtattccta gtgtaatacg ctttcacgaa ttcaccttca
    20761 ggatgaggcg cagtgagctg ttcttcgtga tatagttcca cgcccagtta atgaaaatta
    20821 tgagcttgtt cctgacactc aggatgagca tcaggtgcag gaacatccag aagtaccatg
    20881 ccaggtaacc atggaagctt accagcggga gatcgactat cgccttgttc cttcccacgg
    20941 tagccattga gccgaggtcc ctgtactcgt attctgcaag cttccggcct ttggtcatcc
    21001 gggtaaaatt ccgtgcaagg ttcttgccct ggttgatggc aacgttagcc acctgcggat
    21061 gacccttcgg gtactttggc gtctccatgc aggcaatatc gccaatggca taaatatttt
    21121 cataccccgt caccctgttc gtcctgtcaa cttttatcct gccggcccgg gttataacct
    21181 caggggataa tccgtcgatt atgtttccgg taatcccggc tgtccagata acgtttttgc
    21241 ttttgagaag ctccccgttg ctcagctcaa gggtagagcc gtcatagttc cggacgaaga
    21301 tgccggtcct gagatccacc ccaagcccgg tcaggtattt ttcagacgat cttctggcaa
    21361 gcgggctcat cgtattgaga gtgtggtctc ccccttccag aaggtatatt ttcagccgcg
    21421 agaagtcgat cccgggaaag tccttcggaa ggatatctct tttgatttcg gcgaatgccc
    21481 ctgaaagttc aacccctgtg ggaccccctc ccaccactac aagatttaac aggccctcat
    21541 ctgcattctc tcccgaattg aggatgtttt caaaattctc aagaatcaca ttgcggattc
    21601 tgattgcctc atatgttgat tttaggctga gggtatgttt cccgagctca ctgttgccgt
    21661 aataattggt tttgcctcct gttgcaatga ccaggaagtc atattcaaaa gcacctgctg
    21721 tagtcataac ggacttattt tgcggcttgg cagccagcac atcggccatc ctgacggtga
    21781 tatccttgcg gtcctggaat atctttctca acgggaagga gatacttgtg ggttcgatct
    21841 gcgaagtggc aacctggtaa aacagcggct ggaactggtg gtgattaagc cggtctatga
    21901 gcactacatc aaacattccc ggcttaaggt tcttaatgaa ttgaagcccg gcgaatccgc
    21961 ctccgatcac aatgattctc tttttcatgt caaagaaata atgggttcct gaagataaat
    22021 gaactgcttc ccgtgtgtaa aacaaatgac tacatttatt gtttgttttt aacgcagtca
    22081 acaatttcgg aatgattaaa gtaagaagaa tctatgatcc ggctgaaccc gaagaaggtt
    22141 acagggtact ggttgacagg ctgtggccca ggggcatagc aaaggaaaag gccggctggg
    22201 atgaatggat gagggaggtt gcccccggca atgaattgcg gaagtggtac ggtcatgatc
    22261 cctccaggtg ggaggctttt aaggaaaagt acaggcagga actcagcagc aggaatgacc
    22321 tgcttctgag gctgagagac ctggaacggg cacacggcac gctgacactg ctcttctcat
    22381 cgaaagagct tagtctcaac aatgccgttg ccctcaggga attccttatg cagcaggatc
    22441 gctaatcctg gtacagcttc tgagttttgt caattgacca tcccgttttc aatcccacga
    22501 cgaacagtgc cagggcaacc gagcctacgg cgaatacagt atcaccgata accctgagcc
    22561 agcgcagcgt ctccatggta tcgccctgca tgaagtcggc tgagcgggcg taccacattc
    22621 cgaccttgat gctggcaatt gtctggagca cccccacggg cagcaggctg agaagcagca
    22681 taagcatcag tccgccgttc atggtccaga aggcaatcct gagcattccg gtcttccagc
    22741 ggtattttat tgtcagtccc ttcaggacga agagcatcag ccctatggcc agcataccga
    22801 acacaccgaa cagtgcagcg tggccgtgca acggtgttgt gttgaggccc tgcatatagt
    22861 aaagtgcaat gggcggattg ataaggaagc cgaacaggcc ggcaccaacc aggttccaga
    22921 agctgacaga gataatgaaa tatattggcc atctgtaagc cttgacccag gccgttgcgc
    22981 ggctgagctg caggttatgg t
//
LOCUS       DAOPXU010000039        22884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_2264_length_22884_cov_11.935696, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000039 DAOPXU010000000
VERSION     DAOPXU010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22884
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2264_length_22884_cov_11.935696"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1840)
                     /locus_tag="PKK03_05640"
     CDS             complement(125..1840)
                     /locus_tag="PKK03_05640"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNX83915.1"
                     /translation="MKKTTSLMLSLILMMFIISEKSHACTNFLVTKGATKDGSTMITY
                     SADSHVLYGELYFWPAMKYKPGTMVDVYEWDSGKFLGTIEQAPVTYSVVGNINQHQLA
                     IGETTYGGRGELHNPDGLIDYGSLIYLTLQRAKNAREAIHTIVTLTEKYGYCSSGESF
                     SISDPNEVWIMEMIGKGPGKKGMAFVARRIPDGYISGHANQARIQTFPLANGTTSITS
                     AEIDKVFNPSVETVYAADVVTVARNFGWYDGTDANFSFSDVYAPVDFSAARFCDARVW
                     SGFNKVNSTMGDYLNYAMGYDLENRMPLWIKPDNKLSLEDVMGLMRDYYQDTPMDMTN
                     DVGAGPYANTVRWRPMSWKVDGKNYLHERAISTQQTGFSFVTQSRSWLPDPVGGIIWF
                     GVDDTYYTVYTPMYCGMTSVPRSFAVGNGDIMTYSADAAFWVFNEVTNFVYSRASVMT
                     PDLQAKQKELEHKYMAEIADIDARAAELFKSTAKGAAKKGTEMITAYSVTAGDNTVKE
                     WRELYKHLFTKYMDGNIKTRRPLPADHKYIAPEVKQPGYPQDWLMRIVLDAGDKLKMP
                     DSAGH"
     gene            complement(1937..2677)
                     /locus_tag="PKK03_05645"
     CDS             complement(1937..2677)
                     /locus_tag="PKK03_05645"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HNX83916.1"
                     /translation="MEICADSVESATVAEAAGAGRIELCSALAEGGVTPSAGLLEAAR
                     RNTGIKIHVLIRPRSGDFLYSDSEFSVMRRDIEVAGENGADGIVTGILNRDGTIDVER
                     TALLAEYAAPMSLTFHRAFDLCRDAKQGIEDVIATGAERVLTSGQAKSAIDGAPLIKS
                     LISWSAGRIIIMPGGGIDEYNIGLLAAETGAREYHLSGRRQRESMMTFRRKGIYMGDP
                     RLQSEYILKSADAERISSVIMILKGIVF"
     gene            2760..3659
                     /locus_tag="PKK03_05650"
     CDS             2760..3659
                     /locus_tag="PKK03_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNX83917.1"
                     /translation="MKPTLMVLAAGMGSRYGGNKQLDQVGPSGETIIDYSIYDAKRAG
                     FGKVVFVIRRDIEEQFKETFVKRLRNVIEIDYVFQDLGNLPGGYKVPEGRQKPWGTSH
                     AILVTEPKIHEPFGVINADDYYGYDAFKVLYRFLTEDTNDSNYCIVGYRLGNTLSDHG
                     HVNRGICRVSSDGFLEHMVETRGIEKRPDGVFAPDAAGNINRYTGDEIVSMNLFGFKP
                     SVYKYLNMEFTNFLKERMSDPKAELDIPTSLDKFVKKGEITIQILRSNSHWFGVTYRE
                     DKPFVVESIRKMVDQGMYPEKLF"
     gene            complement(3752..5467)
                     /locus_tag="PKK03_05655"
     CDS             complement(3752..5467)
                     /locus_tag="PKK03_05655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNX83918.1"
                     /translation="MNKRILLLAISITATTVIGSQPSGNVSFVSPLREPPSLSASFAE
                     LRADHFHTGLDYRTGGVQGKDVLAIDEGYVYRIAVSPTGFGRALYVRHPSGYSSVYAH
                     LRSFRPDIEEYVRQQQYGRKSFSVSLFPQRNQFQVGRGEVIAWSGNSGGSSGPHLHFE
                     IRDSASEDPVNPLSFGFGVSDRMRPVIDRVILYPLTRGSAVNSSHGNLSLRTVPSDGS
                     YGIAAATAPVVYGPVGIGIKCWDTFDNSTNKCGVYTIELLADGLRIFSFKADRFSYSE
                     TRYINAHIDYAARAASGEYLHRLHIQPGDRLSMYDGHVGRGVLRFNDDAEHEIKIIVT
                     DMQKNRSWVTFRVRSASKPPVPPAEINCSKVIPWGKAADFTADGIRIHFPAGALYDTL
                     WFNYKTRKNTNGFLSPVHMVHNETVAVHDRFRLSIRPDTIIAGLENKLCLARISSKGA
                     VSYAGGKFTYGFVSGEMNNLGDYAVTLDTIPPAAKFSFASGANLSGRSQFTATIRDEF
                     SGINSYEMYVDGEWILAEYDAKNNLLICRPGTSEMKPDTLHKIELRVTDNCGNRNTVA
                     SEFRW"
     gene            5579..5872
                     /locus_tag="PKK03_05660"
     CDS             5579..5872
                     /locus_tag="PKK03_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83919.1"
                     /translation="MSVQGYEEYLLLRRSVEALIAEHDKLVELAARLNNELSEAGRQL
                     AAKEEEVKELKARYERAKFSGAILGGGEDAVTARRRVSELVREIDKCIALLDR"
     gene            5883..6173
                     /locus_tag="PKK03_05665"
     CDS             5883..6173
                     /locus_tag="PKK03_05665"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNX83920.1"
                     /translation="MDDKLSIRVNVADRYYPLKVESENEERIRKAARLINEKVLQYKQ
                     RYVDKDTQDFLAMAALQYVIRLTEMEEKEEDSSLGDGLKELTEKINLCLSKR"
     gene            6422..7978
                     /gene="rny"
                     /locus_tag="PKK03_05670"
     CDS             6422..7978
                     /gene="rny"
                     /locus_tag="PKK03_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNX83921.1"
                     /translation="MIIAQITTGVCFAIGGAALIAGSVLSFYLWQLMLRNRKKKIIAE
                     AETEAEVIKKEKILQAKERFLQLKAEHEKVINERNNRINQAENRIKQKELTLSQRLEE
                     AQRRKNEVDAIRDNLTQQIELLEKKSEEVSKLHRQQVEQLEAVSGLSADEAKNQLIDS
                     LKEEARTNAMSYINEILDDAKMTATKEAKRVVLMAIQRVAAETAIENAVTVFHIDSDE
                     IKGRIIGREGRNIRALEAATGVEIIVDDTPEAIVLSGFDPVRREIARLALHQLVTDGR
                     IHPARIEEIVEKTRKQVEEEIVETGKRTTIDLGIHGLHPELIRLVGKMKYRSSYGQNL
                     LQHSREVANLASIMAAELGLNPKLAKRAGLLHDIGKVPDDEPELPHAVLGMKIAEKYK
                     EKPEVINAIGSHHDETEMTTLIAPIVQVCDAISGARPGARREVIESYIKRLNELESVA
                     LSYPGVTKTYAIQAGRELRVIVGADKVSDQDTENLSFEIARKIQNEMTYPGQIKITVI
                     RETRAVNYAK"
     gene            7978..9138
                     /gene="wecB"
                     /locus_tag="PKK03_05675"
     CDS             7978..9138
                     /gene="wecB"
                     /locus_tag="PKK03_05675"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNX83922.1"
                     /translation="MKKLKILNIVGARPQIIKASAISRAIAGEFSGEIEEVVVHTGQH
                     YDRELSDVFFDELEIKQPDHNLQVGSGRHGRQTGMIMERIEDVILDEKPDCVIIYGDT
                     NSTLAAALAAAKQHFPVVHIEAGMRSFNKTMPEELNRIMADHASTLLIAPTNAAFKNL
                     MSEGFRPENVAPYSINNPRVFLTGDIMYDNSLFYADLAERKKQDFLTGLGVADREFVL
                     VTIHRDSNTDDPERLSAIFSTIKELSEVNSMPMIMPLHPRTMIALRNRVPELYAAISD
                     HPFLRVIPPVSFLEMILLEKRARLIITDSGGVQKESHFFSRPCIVLRKETEWVELVKN
                     GTVKLADANPARIKEAWDYFIANGSTLQYPAFYGDGKAAIIILKEVLAVFRS"
     gene            complement(9128..9991)
                     /locus_tag="PKK03_05680"
     CDS             complement(9128..9991)
                     /locus_tag="PKK03_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HNX83923.1"
                     /translation="MKKTELIINPDGSVFHLHLLPGDIAEKIIIVGDPGRVEMIAEML
                     DSVRVRKINREFCTVTGSYGETEVTIISSGIGTDNIDIVLNELDALVNIDLGTGMTLE
                     EPRSLTFVRIGTTGGLQAELPVGSFIATAKAVGFDGVLHFYEGYEWCLDHLLADSLAA
                     HLEWPDTLAYPYAVDADTGLLEKLSPGTTRGITISTPGFYAPQGRRLRLEPFDNELND
                     NITSFRFKGQKITNYEMESSAIYGLSRLLNHKALTLCVVIANRVTGEFLTDYKPAVKR
                     LAAQVLEGLSS"
     gene            10164..12650
                     /locus_tag="PKK03_05685"
     CDS             10164..12650
                     /locus_tag="PKK03_05685"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNX83924.1"
                     /translation="MISRAGRILFPAILSIILSLNTVMGASQDKKRAEALRTAGEIQI
                     DGKLDEASWQGASPAGNFIIYNPHNGLPSIYRTEVRLLYDDDALYIGAMMYDSYPDSI
                     FTQLGQRDDGDINADHFWVEISPFNDGLNGEMFKVSASNVQIDNKMTTTDSWGHQDTW
                     DAVWHSRTRISEEGWIAELKIPYSALRFPRSASQLWGINFWREVRRTRETSSWNFVTK
                     EFGSSIAHMGELAGIRDVKPPLRLSLVPYVSGYLEHVTDEPGVGTSYNGGLDLKVGLT
                     ESFTLDATLIPDFGQVQSDDHVLNLSPYEVKYNEKRPFFMEGTELFNKGDIFYSRRIG
                     GTPHLVRDVYDMVGDNEIVTRNPAEVSLLNATKISGRTSSGLGIGIFNAVTNSTWAEV
                     ENNLTGETRHIMTEPLTNYNMLVLDQTLKNDSYVSVMNTSVIRAGEKNENFYTANVSG
                     FEALVKTADRLWSASAGAVLSQKYYSSDPTALGHSLNFNAGRTGGKFRADYNFSMMSD
                     TYDPNDMGYLRNNNETGHSVDFSYNTYEPFGNIMTTRTSLDFSYGQLYNPRAFTTAGV
                     ALDAMILLMNYWSLSLDLELTPWGEDDYFEPRTRDMSMFYHRPAAFEAGFRGDTDKSK
                     RFYIQGSAGYVKAWSDYSQHGYMWSLQPEFKASRRFSFNYSLTVEDVFNDIGFVPGNA
                     GEGITFGKRDVTTITNTISGAFIFSSTSYLTLRGRHYWSRADYDGDYFTLQPDGSLID
                     RVYSTSLNNSDVNTNFLNIDMVYTWRFAPGSELSLVWKNSIWSEGDVIIRNGLDNFGD
                     LLSMPQTNSLSLKILYYLDYQNFRKLFKSK"
     gene            12663..13118
                     /locus_tag="PKK03_05690"
     CDS             12663..13118
                     /locus_tag="PKK03_05690"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HNX83925.1"
                     /translation="MIQRIQTLFLLAVALLSGLMLTGDLIQLTAGGGTVFSMSFLGME
                     NNGGEVIQRLWPMTVILAVVPLLALVTIFLYKKRSLQMRLTMLVLLLSLGTLILGAFY
                     VLMFDRKIDVNIIWKVKVLFPLITAILAWLAWRAILKDEVRVRSYDRLR"
     gene            complement(13111..13578)
                     /gene="pyrI"
                     /locus_tag="PKK03_05695"
     CDS             complement(13111..13578)
                     /gene="pyrI"
                     /locus_tag="PKK03_05695"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HNX83926.1"
                     /translation="MKNVDDPKQLLVSAIESGTVIDHIPASTLFKVIKILGLDRIRNQ
                     ITFGTNLESKTDGKKAIIKIAGKFFEDDDINRIALVAPNAKLNIIRDYQVIEKRVVEV
                     PDTINAIAKCMNPKCITNFEKVTTRFRVVSKKPVALKCLYCEKITDQERLEII"
     gene            complement(13585..14493)
                     /gene="pyrB"
                     /locus_tag="PKK03_05700"
     CDS             complement(13585..14493)
                     /gene="pyrB"
                     /locus_tag="PKK03_05700"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNX83927.1"
                     /translation="MKPRSLVSIDDLTTEEITKILSLAAEFEKTPVQDILRGKVVATL
                     FFEPSTRTRLSFESAVNKLGGKVIGFTDSSSSSVTKGETLNDTIRTVNNYVDLIVMRH
                     PIEGSARYASEISTVPVINAGDGANQHPTQTLLDLYSILKTQGTLENLNICMVGDLKY
                     GRTVHSLLMAMSRWTTSFNFVAPEELKMPDEYKLYLRNLGLDYYETADLNEVINMADI
                     IYMTRVQRERFSDPMEYEKVKNAYVLHDHMLSETKPGMKILHPLPRVNEISTDVDANA
                     KAYYFEQALNGVYTRQAIICTLLGLK"
     gene            complement(14589..15206)
                     /locus_tag="PKK03_05705"
     CDS             complement(14589..15206)
                     /locus_tag="PKK03_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNX83928.1"
                     /translation="MLFSDTYKTIEGESQGLFKDRGSRFIALAVPVASQEEIKTRLAE
                     LRKEYHDARHHCFAWVLGPDRQAWRVSDDGEPSGTAGKPILAQINARELTNVLIVVIR
                     YFGGTLLGVGGLINAYRSATADALGKATVIEKHLMERWSVSFPYTSMNDVMKVFRDES
                     CGQSEHRYSGTDVTIEITFRASMADHVAGRLNKINGLQIKWLATE"
     gene            complement(15231..15980)
                     /locus_tag="PKK03_05710"
     CDS             complement(15231..15980)
                     /locus_tag="PKK03_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNX83929.1"
                     /translation="MITAGNIIALSVIIFFASIVRGFTGFGLALVAVPLIQFILPVTD
                     TAVFIAIINMIFSLLYYRRSRELVREQPLGRMALFTGAGVAAGTLILKYINPAYIQLG
                     WGILIILIVLALARGADFRISSDASAMTLSGLFGGLLAGATGITGPPVAIILSAMKTP
                     KEKFNAVISVFILFAVSYALLFYLISGLIRPETALLALCSVPALLAGLRTGDILVSRI
                     SQKTFTNVIYIVLIIMGIITLYKGAKTLIPV"
     gene            complement(15982..17160)
                     /locus_tag="PKK03_05715"
     CDS             complement(15982..17160)
                     /locus_tag="PKK03_05715"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNX83930.1"
                     /translation="MKVLSGLDVVTLRLPAGLRGKKTGLLCHSSSISSDYRHITEIFG
                     QSKECRLVALFGPQHGLSGQTQDNMIEWEGTIDPVLGIPVYSLYGGHRKPTAGMLKGV
                     ETFLIDLQDVGARLYTYIWTVKHCMEACAETGIPVYILDRPNPIGRIHPDGPVLSEEY
                     FTFVGGACIPLCHRMTIGEMAVWVKEKYIPGCDLHIIRAEGWRRNMMYHETGLPWVLP
                     SPNMPTPHTAAVYPGTVLAEALNISEGRGTVIPFELTGAPWIDGRRLLEEMRGRKLPG
                     CQFRMHDFIPTFHKYAGEYCRGIQIHVTDPSLFRPVATAMHLFDAIIRTSPEGSLKFN
                     PPPYEYEYRLMPFDILSGDSRMREVLTGGLPLKDETRRWEVQTETFMDDFRQIALYPD
                     "
     gene            17283..18581
                     /locus_tag="PKK03_05720"
     CDS             17283..18581
                     /locus_tag="PKK03_05720"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNX83931.1"
                     /translation="MKPVLNSLLSALILISFQAVAQEGITKYIPAAKPERIVLNINGD
                     PATTMAVTWRTDTTIGETVARISRNIPGVLMADSAMAVAGRYEDITADGVTARFHSVI
                     FTGLVPGTEYAYCVGSGADASEWFTFRTAEAGAAPFSFLWFGDSQVGPVSSYARVIRQ
                     AYRSVPEAAFMIFTGDLVDGGYAARTLDDEWGDWHRAAGFIASEVPVVATPGNHEFYN
                     PHDRQNRDFNIYWRPGFTLPENGPAGLEETAYSFDYQGVRFIIVSSDQMLRDEAIARS
                     QTEWVESLLKNNPCKWTVMAFHHPFFSTSARRDNKAVRENLRPLVDRYGVDLVLNGHD
                     HTYARGVMMPDGTERRGRTAGTVYVVSVSGSKQYRQDAEPWWETGYTNTQLWQAITVD
                     GNKLLYKAFDASGTLADQFMLTRMKNGSNRIGLPPAVKTK"
     gene            18586..20322
                     /locus_tag="PKK03_05725"
     CDS             18586..20322
                     /locus_tag="PKK03_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HNX83932.1"
                     /translation="MNMRIPGKLIFIIIAAVLALACTRNEGGLEIYVTTDLHGMLLPF
                     DNTEGESTDRSLANLAWVVESGKKDNMVLLDNGDILQGDPLAYYYNFIDTTSTHIIAD
                     ILNHLGYDAATVGNHDIEAGHAVYDRVRSEYNFPMLAANAIEIKTGVPYFKPYAVIEK
                     KGMKVVVFGLITPSVPRWLPESLYRGIRFENMVATARKWMPAIQAENPDLVIGLFHSG
                     MGDERERGDDENSTLAVAMNVPGFDVIFCGHDHRQDVREVTDSNGDKVLVLDGGSRAE
                     ALMSVSVSFDRTGGGSSTPVLEGRIIEMNTLPVSSGFLKKYRGAMDKVREYTSEVIGR
                     SETTVTTRDAYFGPSAFIDLIHRMQLDITGAQISMAAPLSFDQSISRGELRIRDMYRL
                     YRFENFLYTVRMTGGEIDRHLEHSAGQWFRTMEKGSDYMLNYRTDDNGKPVIVNGTAR
                     LRNPSYNFDSAMGIRYTVDVSKPVGARVNITALSDGSAFSHSASYTVAVNSYRANGGG
                     GHFQAAGIAGKELDRRIISATDRDLRYYMTEWIREKGTISPVPLSQWMIVPAGWAADA
                     EKREMRMLFGNN"
     gene            20334..21440
                     /locus_tag="PKK03_05730"
     CDS             20334..21440
                     /locus_tag="PKK03_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HNX83933.1"
                     /translation="MKILLKIAAFVLVVLTLTSAGSARQKPRNLVLFIGDGMGVSHVY
                     AAMTVSGNTMTFPSFPVTGFSRTHSANSYSTDSAAGGTAIATGEKTNNRMVAMRPDST
                     ILVTLLEHAKAAGMSTGIVCTSSLTDATPADFATHVPLRYEYRAIAKQYMNGAADIFI
                     GGGRKYFVPVPDSTGKINPEEDVVSGLSRAGYDVIYDLESFIASKSNRIAGLMADDDM
                     PRLFEGRDPQYLARATAKAIEVLSRNPRGFILMVEGSEIDDAGHSRSTSMVTDEVLDM
                     DRAVAVAHDFALRDGRTLIVVTADHETGGMSITGGDLAKREVKAEFSFTGHSGVMVPV
                     FAFGPGATEFTGIQENTGLFHNFFRLLSLDRKRR"
     gene            21437..22063
                     /locus_tag="PKK03_05735"
     CDS             21437..22063
                     /locus_tag="PKK03_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNX83934.1"
                     /translation="MRKTETESSGRMLPLVEEFYSVQGEGCNTGRAAYFIRVGGCDIC
                     CSWCDTRYSWNAELHPMVAIESIAGNVSAAGADSVVVTGGEPLMWNMEPLCVLLRSKG
                     IKTFLETSGAYPLTGKWDWITLSPKKNSPPLPGIWEMADELKVVIHDGTDFEEAERYS
                     RNVGPGCQLLLQPEWSMYREMIEAITEYVKKNTRWRVSLQAHKFMHIP"
     gene            22064..>22884
                     /locus_tag="PKK03_05740"
     CDS             22064..>22884
                     /locus_tag="PKK03_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83935.1"
                     /translation="MSAGRTIMALMFLVLTQCSVTGQNLHTRSNRALKYYDQGKRDYE
                     LLYYDRAETNLLQALKEDGKFYEAHLILGQLYSDTGDWGKAVTHYRAAVATDSLFFVP
                     ALYSLGRAELRTGKYYDASIHLDAFLKQPGTTARLRGEAEKMLASCRFALSFPASLFE
                     TEPVSAGDSVNTVLDEYWPSVTGDGQQLIFTREVKRAAGYGRDYNRDRQEDFYVSRLT
                     ADGYWGTARNAGAPLNTAGNEGAQSISSDGRYMFFTACDRNDGQGRCDIYFSSYDG"
BASE COUNT         5722 a         5507 c         6095 g         5560 t
ORIGIN      
        1 tactactttc attaagtcca tcgcgcaaat atgtttatgt gatctttact ctttttaaac
       61 aggtcgcaaa ggtagcaata tttttttatt atctgcaaaa tatttattgc aattgcagta
      121 aatatcagtg acctgcgctg tcaggcattt tgagtttatc gcctgcatcg agtacgattc
      181 tcataagcca gtcctgcggg tagccaggct gtttaacctc gggagcgata tacttatggt
      241 ctgccgggag aggacgacgg gttttgatgt tgccatccat gtatttggta aagaggtgtt
      301 tatatagctc tctccattcc tttaccgtgt tgtcgccggc ggtgaccgag taagctgtaa
      361 tcatttcggt tccctttttg gcagcgcctt tggcagtgct tttaaagagc tcggcagccc
      421 tggcatctat atccgcaatc tcagccatat acttatgttc aagttccttc tgttttgcct
      481 gcagatcggg tgtcatgacg ctggcgcggc tgtatacgaa attggtgact tcattgaata
      541 cccagaaggc agcgtcggcg ctgtaggtca tgatgtcacc gttgcccacc gcaaatgaac
      601 ggggaacgga ggtcattccg caatacatcg gggtatagac tgtatagtag gtgtcatcaa
      661 caccaaacca gatgatgccg cccacagggt cagggagcca tgaacggctc tgggttacga
      721 acgaaaagcc tgtctgctgg gttgagatgg ctctttcatg cagatagttc tttccgtcaa
      781 ccttccagct catgggcctc caccttacgg tattggcata cgggcctgcg ccgacatcat
      841 ttgtcatgtc cattggggtg tcctggtaat agtccctcat aaggcccatt acatcttcca
      901 ggctgagctt gttgtcaggc tttatccaga gaggcatcct gttctcaagg tcgtatccca
      961 tggcataatt aagataatca cccatagtgc tgttgacctt gttgaatcct gaccatacgc
     1021 gggcatcgca gaaacgtgcg gcactgaaat caacaggggc ataaacatca ctgaagctga
     1081 agtttgcatc ggtgccgtca taccatccga agttccgtgc aacagtcacg acatcagcag
     1141 cgtatactgt ctcaaccgaa gggttgaata ccttgtctat ctctgcggat gtgattgatg
     1201 tggtgccatt ggccaggggg aaggtctgta tccttgcctg gttggcatgt cctgagatgt
     1261 aaccgtccgg aatcctgcgg gcgacaaatg ccattccctt cttgccgggg cccttcccga
     1321 tcatctccat gatccacact tcgttgggat cagatatgga gaatgattca cccgaactgc
     1381 aataaccata tttttcggtc agtgtgacaa tggtgtgtat agcttccctt gcgttcttcg
     1441 ccctctgcag cgtaaggtaa atcaggctgc cgtagtcaat taatccgtcg ggattgtgca
     1501 gttcgccgcg tccgccgtag gtggtctcgc caatagccag ctggtgctgg ttaatgttgc
     1561 cgaccaccga gtaggtaacg ggtgcctgtt caatagtacc caggaatttg cctgagtccc
     1621 actcgtatac gtcaaccatt gttccgggtt tatacttcat tgcgggccag aagtaaagct
     1681 cgccgtaaag gacgtgtgag tcggccgaat aggtgatcat ggtggaaccg tctttagtgg
     1741 ctcccttggt gaccaggaag ttggtgcacg cgtggctctt ctcagaaata atgaacatca
     1801 tgagaatgag gctcagcatc agtgatgtgg ttttcttcat gtttattgga ttgatttaat
     1861 ttgttatatt ataatcaatt actgctccgt ccccgctttc tgcggaccgg aaagctctca
     1921 aaaatagcat ctttaattaa aaaacaatac cctttaaaat cattattacc gaactgatcc
     1981 gttcggcgtc agcgctttta aggatatatt ccgactgcag cctgggatcg cccatgtaga
     2041 taccttttct gcggaaggtc atcatgcttt ccctctgccg gcggccggag aggtgatatt
     2101 cccttgcgcc ggtttctgca gccagcaggc ctatattgta ttcgtcaatg cctccgcccg
     2161 gcataatgat gattcttcct gccgaccatg aaataaggct tttaatgaga ggggcgccgt
     2221 cgatggcgct tttggcctga ccggaagtaa gaactctctc cgcgccggtg gcaattacat
     2281 cctcaattcc ctgtttcgcg tccctgcaga ggtcgaatgc ccggtgaaag gtgaggctca
     2341 tcggcgccgc atattctgcc aggagggctg tccgctccac atctattgtt ccatccctgt
     2401 tgaggattcc ggtgacaatt ccgtcagcac cgttctcgcc ggcaacttca atgtcacgcc
     2461 gcattacact gaactcactg tcactgtaaa ggaagtcgcc gctgcgcggc ctgatcagaa
     2521 catggatttt gattccggtg ttgcgccgtg ccgcttcgag aagaccggca cttggagtga
     2581 ctcctccctc ggcaagggca gaacacaatt caatcctccc ggctccggca gcttcggcaa
     2641 cagtggctga ctcaacggag tcagcacata tttccacaac aagggacaat tcttttctat
     2701 gaggctttga aagtacatat tatttagata atattgtgtg acctaaaaca tttaatcaga
     2761 tgaaacctac actaatggtt ctggctgccg ggatgggcag cagatatggc gggaacaagc
     2821 agcttgacca ggtcgggcct tcaggcgaaa cgatcattga ctattcgatc tatgatgcca
     2881 agagggccgg attcggcaag gtggtgtttg tcataaggcg tgatatagag gagcagttca
     2941 aagagacctt tgtaaagaga ctcaggaatg taatagagat tgattatgtt tttcaggatc
     3001 tcggaaacct tcccggcgga tacaaggtgc ctgaaggacg tcagaagccc tggggcacaa
     3061 gtcatgccat cctggtgacg gaacccaaga ttcacgagcc tttcggggta ataaacgccg
     3121 atgactacta tggatatgac gcctttaagg tgctttacag gttcctcaca gaggatacca
     3181 atgacagtaa ttattgcata gtgggttatc gtctgggcaa caccctttct gaccatggtc
     3241 atgtcaaccg cggcatctgc agggtaagca gtgacggatt ccttgagcat atggtggaaa
     3301 ccaggggaat agaaaagaga cctgacggcg tttttgcccc ggatgcagca ggaaacatca
     3361 acaggtatac cggcgacgag attgtctcca tgaatctctt cgggttcaag ccctcggttt
     3421 acaaatacct gaacatggag ttcaccaact tcctgaagga gcgtatgtct gatcctaagg
     3481 ctgaacttga cattcctacc tcccttgaca aattcgtaaa gaaaggggag atcaccattc
     3541 agatcctgag gagcaacagc cattggtttg gtgtcaccta cagggaggac aaaccatttg
     3601 ttgttgaaag catcaggaag atggtagacc agggaatgta cccggaaaaa ttgttttaac
     3661 ctgggggaaa aataatccgc atacatatgc cgtagagagg ggtctcagac ccctcgtttc
     3721 ctgtatgata ggatgatccg catatatatg attaccacct gaattcggat gccacggtat
     3781 tgcggttgcc gcaattgtcg gtaacccgca gctcaatttt gtgcagcgta tctggtttca
     3841 tttcagatgt cccgggcctg catatgagca ggttattctt ggcatcatac tcagccagta
     3901 tccattctcc gtcaacatac atctcgtagc tgttgattcc tgaaaactcg tcgcgtatgg
     3961 ttgcagtgaa ctgcgacctt ccgctaaggt tggcgccgct tgcaaatgag aactttgcag
     4021 ctggtggaat ggtatcaagg gtgacggcat aatctccaag gttgttcatc tctccgctca
     4081 cgaagccgta ggtgaatttt cctcccgcat atgagacagc tcccttgctg ctgatcctgg
     4141 ccaggcacag cttgttctca agaccggcaa ttattgtgtc aggccttatt gacagcctga
     4201 acctgtcatg tacggctaca gtctcattat gaaccatatg gacaggggat aggaatccgt
     4261 tggtgttttt ccgggttttg tagttgaacc acagcgtgtc atagagcgcc cctgcgggga
     4321 aatgaatcct tatcccgtca gcggtgaaat cagcagcttt gccccacgga atcaccttgc
     4381 tgcagttaat ctctgcggga ggaaccgggg gttttgatgc tgacctgacc cggaaggtga
     4441 cccacgacct gttcttctgc atatcagtaa cgatgatttt tatctcatgt tcagcgtcat
     4501 cgttgaatct cagaactccc cgtcccacgt ggccgtcata cattgaaagc ctgtcgcctg
     4561 gctgtatatg cagcctgtgc agatattcgc ccgaagcggc ccgtgcagca taatcaatat
     4621 gtgcgttgat gtacctggtc tcgctgtatg agaaacggtc ggctttgaag ctgaatattc
     4681 tcagtccgtc ggccagcaat tcaattgtat atacaccaca tttgttagtg ctgttgtcaa
     4741 aggtgtccca gcacttgatg ccgatgccca cagggccgta aacaaccggt gcggttgcgg
     4801 ctgcgatgcc atatgatccg tcagaaggta cggtgcgcag cgagaggttg ccatggctgc
     4861 tgttgacagc tgaacccctt gtcagcggat aaaggataac cctgtcaata accggccgca
     4921 tgcgatcact cacgccaaat ccgaaactga gcggattgac ggggtcttca gaagctgagt
     4981 cacgtatctc gaaatgcaga tgcggcccag acgagcctcc cgagttgcct gaccatgcga
     5041 tcacctcgcc gcggcccacc tggaactggt ttcgctgggg aaaaagagag acggagaagc
     5101 tcttgcggcc gtactgctgc tgcctgacat actcctcgat gtcaggcctg aaactcctta
     5161 gatgtgcata aactgatgaa tagccggacg ggtgccggac atacagggct ctgccaaaac
     5221 cggtagggct gactgcaata cggtagacat agccttcatc aattgccagc acatccttgc
     5281 cctgaactcc cccggtacgg taatccagcc cggtatgaaa atggtcggct ctcagttctg
     5341 caaagctggc cgagagcgaa ggaggttccc tgagggggct tacaaatgat acatttccgg
     5401 aaggctgtga accaatgaca gttgtggctg ttatagaaat ggcaaggagt aatattcttt
     5461 tgttcatgtc ggtgtcagtt ctgcaaatct attgttctgc ccgtaaagag caaacttaat
     5521 gccggctttt tttgaaaaga gcgaaaaaaa tgttattttt gtattatagt tgtcagccat
     5581 gagtgtacag ggatatgagg agtatctttt gctcaggagg tcagtggaag cgctgattgc
     5641 cgagcatgac aagctggttg agctggcggc gcggttgaat aatgaactgt cagaggcggg
     5701 gaggcaactg gcagcgaagg aagaagaagt aaaggagctt aaagcaagat acgaaagagc
     5761 taagttctcg ggagcgatcc tggggggcgg ggaggatgcc gttacggcac ggaggagagt
     5821 gagtgaactt gtgcgggaga tagataaatg tattgctctt ttagacagat aaatatataa
     5881 caatggatga taagctttcg atccgggtta atgtggccga caggtattat ccgttgaagg
     5941 tggagagtga gaatgaggaa cgtatcagga aggcggccag gctgatcaat gagaaggtgc
     6001 tgcagtacaa gcagcgctac gttgacaagg acacgcagga ttttctggcg atggcggctc
     6061 tccagtacgt gatcaggctc actgagatgg aagaaaagga ggaggattca tcacttgggg
     6121 atgggttgaa ggagctgact gaaaagatca acctctgtct gtcaaaaaga tagatacacg
     6181 ttctttaaaa tagcaggata ttgcccgcat tgtttctcta ttcgttttgg aaactcaaca
     6241 gtttttactt tggagatggt cttggattgc ttaggacagg cctcacccgg ttcgccggga
     6301 ccacgctgac gcgtgacagt ggaagcccga ggcaggacgg ccgctccagc catgtagata
     6361 tggggttttt taaaagttgg aggaactctg cgggtttttt tatacaacta acaatagttt
     6421 tatgattata gcacagataa caacgggggt ttgtttcgcc atcggcgggg cagcccttat
     6481 agcaggatcg gtgttgtcat tctacttatg gcaactgatg ctgcgaaaca ggaagaagaa
     6541 gataattgcc gaagcagaaa ctgaagccga ggtcataaaa aaggagaaga ttctccaggc
     6601 aaaggaacgg ttcctgcagc tgaaggctga acatgaaaag gtaatcaacg agcgtaacaa
     6661 ccgcataaac caggcagaga acaggatcaa gcagaaagag cttaccctgt cacagagact
     6721 ggaagaggca cagcggagga agaatgaggt tgacgccatc cgcgacaacc tgacacaaca
     6781 gattgagctg ctcgagaaaa aatcagagga agtatcaaaa ctgcaccgcc agcaggtgga
     6841 acagttggaa gcagtatcag gtctctcggc agatgaggcc aagaatcagc tcattgattc
     6901 actgaaggag gaagcgcgca ccaacgccat gtcttacatc aatgagattc ttgatgatgc
     6961 taagatgact gccacaaagg aggcaaagcg tgttgtgctt atggccatac agcgtgttgc
     7021 agcagagacg gcaattgaaa acgccgtaac cgtattccat atagactcgg atgagatcaa
     7081 gggccgcatc attggccgtg aaggaaggaa tatcagggct cttgaggctg caacgggggt
     7141 ggagataata gtggatgaca cccccgaggc aatagttctt tccggattcg accctgtcag
     7201 acgcgagata gcgcggctgg cgctgcatca actggtcacc gatggccgca tacacccggc
     7261 gcgtatcgag gagatagtcg agaagacacg caagcaggtt gaggaagaga tagtggaaac
     7321 gggcaagcgc acgaccatag accttgggat tcatggcctc catccggagc tcatcaggct
     7381 ggtgggaaag atgaaatacc gttcatcata cggacagaac ctgctgcagc actcccgtga
     7441 agtggccaac ctggcttcaa ttatggcagc tgagctgggt cttaacccga agcttgcaaa
     7501 gagagcagga ctgctgcatg atattggcaa ggtgcccgat gacgagcccg aactgccgca
     7561 tgctgtcctt ggaatgaaga tagcagaaaa atataaggag aagcctgagg tgataaacgc
     7621 catcggatcg catcacgatg agacagagat gaccacactc attgcaccca ttgtgcaggt
     7681 atgtgacgcc atctcaggcg ctcgtccggg cgcaaggcgc gaagtcatag aatcttacat
     7741 caaaaggctt aacgaacttg agtcggttgc cctctcttat cccggcgtga caaaaacata
     7801 cgcaatccag gccggaagag agctgagggt tatagtggga gctgacaagg tgtctgacca
     7861 ggatacagag aacctctcct tcgagattgc aagaaagatt cagaacgaga tgacctatcc
     7921 gggtcagatc aaaataaccg ttatcaggga gacaagggca gtcaactacg ccaaataatg
     7981 aaaaaactca agatccttaa catcgtcggc gcgaggcctc agatcataaa ggcctccgcc
     8041 atcagccggg cgatagcggg agagttctca ggtgagattg aggaggtggt ggttcatacc
     8101 ggtcagcatt atgacaggga actctcagac gtcttttttg acgagctgga gataaagcag
     8161 cctgaccaca acctgcaggt agggtcagga agacatggca ggcagacggg aatgattatg
     8221 gaacgcatcg aggatgtgat ccttgacgaa aaacctgact gcgtgattat ctacggtgac
     8281 acaaactcca ccctggccgc agcgctggca gcagcaaagc agcatttccc ggtggtgcat
     8341 attgaagccg gcatgagatc attcaacaag accatgcctg aggagctcaa ccgcatcatg
     8401 gctgaccatg cctcgacgct tctgatagct cctacaaacg cagccttcaa gaaccttatg
     8461 tcagaaggct tccgcccgga gaacgtggcg ccgtattcaa tcaacaatcc cagggtcttc
     8521 ctgacaggtg acatcatgta tgacaacagt ctcttttatg ctgacctggc agagaggaaa
     8581 aaacaggatt tccttaccgg actgggtgta gccgaccggg aattcgtgct ggttaccatc
     8641 caccgtgact cgaacacgga tgatccggag aggctcagcg ccatattcag caccataaag
     8701 gagttgtctg aggtaaactc aatgcccatg atcatgccgc tccacccgag aacaatgatt
     8761 gcattgcgta accgtgttcc ggagctatat gcagcaatat ccgatcaccc cttcctgagg
     8821 gtcataccac cggtctcatt ccttgaaatg atactgctgg aaaagagggc tcgactcatc
     8881 atcaccgatt caggaggtgt gcagaaggag agtcatttct tctcacgtcc ctgcattgtg
     8941 ctcaggaagg agacagaatg ggtggaactt gtcaaaaacg gaaccgttaa gctggcagat
     9001 gccaatccgg ccaggataaa agaagcctgg gactatttta ttgccaacgg cagtacgctg
     9061 cagtacccgg cgttctacgg agacgggaaa gccgcaatta ttattctgaa agaggtactc
     9121 gcagtattca ggagctgagt ccttcaagaa cctgcgcggc aagtcttttg actgccggtt
     9181 tgtagtcggt gaggaattca ccggtcaccc tgtttgcaat taccacgcag agcgtcaacg
     9241 ccttgtgatt caatagtctt gacagtccgt agatggctga actctccatc tcgtagttgg
     9301 tgattttctg tcctttgaac ctgaatgatg tgatattgtc attaagctca ttgtcaaatg
     9361 gttcaagcct gagccggcgg ccctgcggtg catagaagcc aggtgtcgat atggtgatcc
     9421 cccttgttgt tcccggggat agtttctcaa gcagaccggt atctgcgtcc acggcatagg
     9481 ggtaggcaag agtgtcaggc cattcaagat gtgcagccag tgagtcagcc agcagatggt
     9541 caaggcacca ttcatatccc tcgtaaaaat gcagaacgcc gtcaaatcca acagctttgg
     9601 cggtggcaat gaaggaacct acgggcagtt ccgcctgaag tccgcccgta gtgccgatcc
     9661 ttacaaatgt cagtgaacga ggttcttcca aagtcatccc ggtcccgagg tcgatattca
     9721 ccagtgcgtc aagctcgttg agaacgatat cgatattgtc agtgcctatt cctgatgata
     9781 tgatggtgac ctcagtttca ccgtagctgc cggtgactgt acagaactcc ctgttgattt
     9841 tacgtaccct cacgctgtca agcatttcgg caatcatctc aacccttccg ggatctccca
     9901 caataattat cttttcggca atgtctccgg gaagaagatg gaggtgaaac actgagccgt
     9961 caggattgat tataagttct gtctttttca tcgcacgatc aataagctgc aaaaatacaa
    10021 aaaatgtcga ctgtaaatca tctgccggga agatggcgca ggcagttaat tattgttaat
    10081 ttgacaattg catcatcagg gtttatcgca ggggtttatc tttgccgtcc taaccgacat
    10141 acactaaaat gcattttccc aaggtgatat ccagggccgg caggatactt tttccggcaa
    10201 tactgtcaat cattctgagt ctgaacactg tgatgggagc ttcccaggat aagaagcggg
    10261 ctgaggcttt acgcactgca ggggaaattc agattgacgg gaagcttgat gaggcgtcat
    10321 ggcagggagc ttcacccgcc gggaatttca ttatttacaa cccgcataac ggcttgccat
    10381 cgatatacag gaccgaggtg aggcttcttt atgatgatga tgcattatat atcggagcca
    10441 tgatgtatga cagttatcct gacagcatct tcacccagct tggccagaga gatgacggcg
    10501 atatcaatgc agaccatttc tgggttgaga tatcgccctt caatgacggg ctaaacggtg
    10561 agatgttcaa ggtctcagcc tcgaacgtgc agattgataa taagatgacc actactgaca
    10621 gctggggaca ccaggataca tgggatgcag tctggcacag caggacgagg ataagtgagg
    10681 agggttggat tgcagagctg aagataccct actcggccct gcggtttcca aggtctgcat
    10741 cgcagctctg gggcatcaac ttctggcgtg aagttcgccg caccagggag acctcttcct
    10801 ggaatttcgt cacgaaggaa ttcgggtcat caatcgcaca catgggtgag cttgccggga
    10861 tacgtgatgt aaaaccacca ctgaggcttt cgcttgtgcc atatgtttca gggtaccttg
    10921 aacatgtgac cgatgaaccg ggagtgggca cctcatataa cggggggcta gacctcaagg
    10981 tcggcctgac cgagagcttc acccttgatg caacgctgat acccgatttc ggacaggtgc
    11041 agtctgatga ccatgtgctt aacctgtcgc cttacgaggt caagtacaat gaaaagagac
    11101 cattcttcat ggagggtaca gaactgttca ataagggaga catcttctat tcccgccgca
    11161 taggcggaac acctcacctg gtaagggatg tgtatgacat ggtaggtgat aatgagatag
    11221 ttacccggaa tccggcagag gtgagtctgc ttaatgcgac caagatatca ggaaggacaa
    11281 gcagcggcct cggcatcggt atcttcaacg cggttaccaa cagcacctgg gccgaggtgg
    11341 agaacaatct caccggggaa accaggcata tcatgaccga gccgctgaca aactacaata
    11401 tgcttgtgct tgaccagacc ctgaaaaacg actcatacgt gagtgtaatg aacaccagcg
    11461 tgatcagggc cggtgaaaag aatgaaaact tctatactgc caacgtctcc ggatttgagg
    11521 ctcttgtcaa gacggccgac aggctgtggt cagcctccgc cggagctgtg ctgagccaga
    11581 agtactactc ttccgatccc accgctctcg gtcattcact taacttcaat gccggcagga
    11641 ccggcgggaa gttccgcgct gactacaact tttcaatgat gagtgacacc tatgatccga
    11701 atgacatggg ttacctccgg aacaataacg aaacagggca ctcggttgac ttctcatata
    11761 atacctatga gccctttggc aatatcatga ccacccgtac ctcactcgat ttcagttacg
    11821 gccagctata caatccgagg gcattcacaa ctgccggtgt tgcgcttgac gcgatgattc
    11881 tgctgatgaa ttactggtct ctctcacttg accttgagct cactccgtgg ggagaggatg
    11941 attattttga gccgcgcacc cgtgacatgt caatgtttta tcaccggcct gctgcattcg
    12001 aagccgggtt ccgcggtgac accgacaaaa gcaaacggtt ttacattcag ggaagtgccg
    12061 ggtatgtgaa agcctggtca gactacagcc agcacggtta tatgtggtca ctccagcctg
    12121 agttcaaggc cagcaggcgt ttttccttca attactcact gacggttgaa gacgtattca
    12181 atgatatcgg gttcgtgccg ggcaatgcag gcgagggaat cactttcgga aaacgtgatg
    12241 tcacaaccat aaccaatacc atttcggggg cattcatctt ctcatccacc tcgtacctta
    12301 cactgagggg gaggcactac tggtcaagag ctgattatga cggggattat tttacccttc
    12361 agcctgacgg aagcctgatt gaccgggtct attccacatc actgaacaac agtgacgtaa
    12421 acaccaattt cctcaacatc gatatggtat atacctggcg gtttgctccg ggaagtgaac
    12481 tttcactggt atggaagaac tccatctggt cagaaggtga tgtcatcatc cgtaacgggc
    12541 tcgacaattt cggtgatctt ctctccatgc cgcagacaaa cagcctgtca ttaaaaattt
    12601 tatactacct tgactaccag aatttcagaa aactcttcaa gtcaaagtaa ttaactttca
    12661 gtatgataca aagaattcag acattgtttc tgctggcagt ggcgctgttg tcggggctga
    12721 tgctcaccgg cgaccttatc cagctgacgg ccggtggtgg caccgttttc agcatgagtt
    12781 tcctgggaat ggaaaacaac gggggagagg tgatacaacg gctttggccg atgactgtta
    12841 ttcttgcagt ggtcccgctc cttgcactcg ttaccatatt cctctacaaa aagaggtcat
    12901 tgcagatgag gttaacgatg ctggtcctcc tgctttcgct cggaaccctg atattgggag
    12961 ctttttatgt gctgatgttt gacaggaaga tagatgtgaa catcatctgg aaggtaaaag
    13021 tcctctttcc gctcataact gccatcctgg cgtggctcgc ctggcgcgcc atactgaagg
    13081 atgaggtcag ggtcaggtca tatgaccgcc tcagatgatc tccagtctct cctggtcagt
    13141 gattttctcg cagtaaaggc atttcagcgc caccggtttc tttgacacca ccctgaagcg
    13201 tgttgtcacc ttctcgaagt tggtgatgca tttgggattc atgcatttgg ctattgcatt
    13261 gatggtgtca gggacctcca caacgcgctt ttctatcacc tggtaatcgc ggataatatt
    13321 cagcttcgca ttgggggcca caagggcgat tctgtttata tcatcatcct cgaaaaattt
    13381 ccctgcaatt tttatgatag cctttttgcc gtcagttttg ctttcgaggt tggtgccaaa
    13441 ggttatctgg ttcctgatac ggtcgagacc gaggatcttt attaccttga aaagagtgct
    13501 ggcagggatg tgatctatca ctgttccgct ttcaatggcg ctgaccagca actgcttcgg
    13561 atcatctacg ttcttcatgg cttgttattt taatccaagt aatgtgcata tgattgcctg
    13621 cctggtgtaa accccgttga gggcctgttc gaaatagtag gctttcgcgt tagcgtcaac
    13681 atcggtgctg atctcattca cacgcggcag cggatgcagt atcttcattc cgggtttggt
    13741 ttctgacagc atgtgatcat gcaacacata ggcattcttg accttttcat actccatagg
    13801 gtcagagaag cgctcacgct ggacacgcgt catatagata atgtcagcca tgttgataac
    13861 ctcgttcagg tcagcagttt cataatagtc aaggccaagg ttcctcaggt agagcttgta
    13921 ttcgtcaggc atcttcagtt cttccggcgc aacaaaattg aacgaagtcg tccaccgtga
    13981 catggccatc agcagggagt gaacggtgcg gccatacttc aggtcaccca ccatgcagat
    14041 attcagattt tcaagcgtac cctgagtctt cagtatagag tagagatcga gaagagtctg
    14101 tgtgggatgc tgattggccc cgtcgcctgc attgatgaca ggcaccgtcg atatctcgct
    14161 tgcatagcgt gcacttcctt caatgggatg ccgcatcaca atcaggtcaa cgtaattgtt
    14221 gacggtcctg attgtatcat tcagagtctc acctttggta acacttgaac tggaagagtc
    14281 agtgaatcct atcacctttc ccccgagctt gttgacagca ctctcaaaac tgaggcgtgt
    14341 gcgggtggaa ggttcgaaga agagcgttgc cacaaccttg ccacgaagta tatcctgaac
    14401 cggagtcttc tcaaattccg cggccagact gagtatcttt gtgatctctt cggtggtcag
    14461 atcatcaatt gagactaagc ttctgggttt cattatttta tgttttatgt gcctgaactc
    14521 tggcaaagtt attataactg tcccagcaat tttctgtctg tttaaaacta tttaaaaaaa
    14581 cagcccgact attctgttgc gagccatttt atctgcagcc cgttgatctt gttcagcctg
    14641 cctgccacat ggtcagccat ggaagccctg aacgtgatct caattgtcac gtcagtaccg
    14701 ctgtatcggt gttccgactg gccacatgat tcatccctga acactttcat gacatcattc
    14761 atcgaggtat agggaaagga gacggaccat cgttccatga ggtgtttctc aatcaccgtt
    14821 gctttaccaa gagcatcggc agttgcgctg cggtaagcgt ttatcagtcc gccaacgccc
    14881 agcagggttc ctccgaaata cctgattacc actatgagga catttgtcag ttcgcgggca
    14941 ttgatctgtg caaggatcgg ctttccggcg gtgcccgacg gctcaccgtc atcactgacg
    15001 cgccatgcct gcctgtcggg ccccagtacc catgcaaagc aatgatgcct cgcatcatgg
    15061 tactcctttc tgagctccgc gagcctggtc tttatctcct cctgagaggc cacgggaaca
    15121 gccaatgcga tgaacctgct gccacggtct ttgaagagcc cctgcgactc tccctctata
    15181 gttttatatg tatctgaaaa gagcatattt ttatgcgaag cctgggtaat ttaaaccgga
    15241 atcaacgtct tcgctccctt gtaaagcgtg attatgccca tgattatcag cactatatat
    15301 atcacattcg taaatgtctt ctggcttatt cgcgaaacga ggatatcacc tgtgcggaga
    15361 ccggcaagga gggccggcac cgagcataag gcaagcaacg cggtctccgg gcggatgagt
    15421 cctgatatca ggtagaacag cagggcatat gaaactgcaa agagaatgaa gacggagatc
    15481 acagcgttga atttttcctt tggcgtcttc attgcagaga ggattatggc cacgggtgga
    15541 ccggttatgc cggtagcccc tgcaaggagg ccgccgaaaa ggccgcttaa cgtcatggcc
    15601 gaggcatcgg aactgatcct gaaatcagct cccctggcaa gggcaagtac aatcaatatt
    15661 atcaggattc cccaccctag ctgaatatat gccgggttga tgtatttaag gatcagtgtt
    15721 ccggccgcaa caccggctcc ggtaaacagt gccattcttc ccaggggctg ctccctgaca
    15781 agttcccttg accggcggta gtagagaagg gagaagatca tgtttatgat ggcaatgaag
    15841 acggcagtat ctgttacggg aagaatgaac tgtatcagcg gcactgcaac cagtgccagc
    15901 ccgaagccgg tgaatcctct gacgatggaa gcgaagaaga ttattactga aagggcaatg
    15961 atatttccgg cagtgatcat cttaatccgg gtatagggct atctgcctga agtcatccat
    16021 gaatgtttcc gtctgcactt cccagcgccg ggtttcatcc ttcagcggga ggccgccggt
    16081 cagcacttcg cgcatgcggc tgtcgcccga tagaatgtcg aaaggcatca ggcggtattc
    16141 gtactcatac ggtggaggat tgaatttcag ggagccttcc ggcgatgtcc ggatgattgc
    16201 atcgaacagg tgcatggcag ttgccacagg cctgaagagg gagggatcgg tgacatggat
    16261 ctgtattccc ctgcagtatt cgccggcgta tttatggaag gttgggatga agtcatgcat
    16321 cctgaactga catccgggga gttttctgcc tcgcatttcc tcaagaagcc ttcttccgtc
    16381 gatccaggga gctcccgtga gctcaaaggg tatcactgta ccccgtccct cggagatatt
    16441 aagggcttcg gcgagcactg ttcccgggta gactgctgcc gtatgggggg ttggcatgtt
    16501 cggagagggg aggacccatg gcaggcctgt ctcgtgatac atcatattcc gtctccatcc
    16561 ttccgccctg atgatgtgca gatcgcaacc gggaatatat ttctctttca cccatacggc
    16621 catctcgcct attgtcatgc ggtggcagag aggaatgcag gcaccgccga cgaatgtaaa
    16681 gtactcttcg ctcaaaaccg gtccgtcagg atgtatcctt ccgatagggt tgggcctgtc
    16741 aagaatgtat acaggtatgc cggtttcggc gcatgcctcc atgcaatgct taactgtcca
    16801 gatatatgta tatagtcttg ctccaacatc ctgaagatca atcagaaatg tttcaacgcc
    16861 cttaagcatt ccggccgttg gcttgcggtg cccgccataa aggctgtaga cgggaatgcc
    16921 aagtacaggg tcaatggtcc cttcccactc tatcatgttg tcctgggttt gccctgagag
    16981 cccgtgctgc ggcccgaata gggcgacaag cctgcactct ttgctttgcc cgaatatctc
    17041 tgtaatatgc ctgtaatctg atgatatgct cgaagaatgg cacaacagtc ccgtcttctt
    17101 accccggagg ccggcaggca gcctgagggt cacaacgtca agtcctgata acactttcat
    17161 acaattacaa aaatagttaa aagagccgtg gcagcaagat cggtctgccg ctctgcatgg
    17221 aaattatcaa tatatttaca acctcccgta acggggaatt gttaaagtat taaaaccaaa
    17281 tgatgaagcc tgttctgaac tctctcctgt ctgcactgat attgatttca ttccaggcag
    17341 tggcgcagga gggaattaca aaatatattc ccgctgcgaa acctgaaagg attgtactta
    17401 acattaacgg agatccggcc acaaccatgg ctgtgacctg gagaacggat accacgattg
    17461 gcgagacagt ggcaaggatt tcccgcaaca ttcccggcgt gctgatggct gactctgcca
    17521 tggctgtggc aggaagatat gaggatatca ctgctgacgg agtgacggcc cggtttcatt
    17581 cggtcatatt caccgggctg gttcccggaa ccgaatatgc ttactgcgtt gggtccggcg
    17641 ctgatgccag cgagtggttt accttcagaa ccgctgaagc aggtgcagcc ccgttctcct
    17701 ttctgtggtt cggagattca caggtggggc cggtttcttc ctacgcgagg gtgatcaggc
    17761 aggcttaccg ctcggtgcct gaagcagcat tcatgatttt tacgggtgac ctggttgacg
    17821 gcgggtatgc tgccagaact cttgacgatg agtggggtga ctggcaccgg gcggcaggat
    17881 tcatcgcatc ggaggtgccg gtggttgcta cccccggaaa ccatgaattc tacaacccgc
    17941 acgacagaca gaaccgtgac tttaacattt actggagacc gggtttcact ctcccggaaa
    18001 acggacctgc cggactggag gaaacagcat acagtttcga ctaccagggg gtgcggttca
    18061 taatagtcag cagcgaccag atgctgaggg atgaagccat tgcccgcagt cagactgaat
    18121 gggttgaaag ccttcttaaa aataatccgt gcaaatggac cgtgatggca tttcatcatc
    18181 cgttcttttc aacgtccgcc aggagagaca ataaagctgt cagggaaaac ctgagaccgc
    18241 tggttgacag gtacggcgtt gacctggtac ttaacggtca tgaccataca tatgccaggg
    18301 gagtcatgat gcctgacgga actgaacgca ggggcaggac ggccgggacc gtttatgtgg
    18361 tctcggtcag cggctcaaaa caatacaggc aggacgccga accatggtgg gaaacagggt
    18421 ataccaatac ccagttatgg caggcaatta ctgttgacgg caacaaactt ttatataaag
    18481 catttgatgc gtcaggcacc cttgccgacc agttcatgct caccaggatg aaaaatggca
    18541 gtaaccggat agggttgccg ccggcagtaa aaacaaaata ataccatgaa tatgagaatc
    18601 cccgggaagc tgatatttat aatcattgcc gctgtacttg cgcttgcatg cacaagaaat
    18661 gagggcgggc ttgagatata tgtcaccact gacctgcatg gaatgctgct cccctttgac
    18721 aataccgaag gtgaatccac tgaccggtcg ctcgccaacc tcgcatgggt ggttgagtcg
    18781 ggcaaaaagg acaatatggt tctgcttgac aacggtgaca tactgcaggg tgatccgctg
    18841 gcatactatt ataacttcat cgatactacc agtacccaca taatagctga catacttaac
    18901 cacctgggct atgatgcagc tactgtgggc aaccatgaca tagaggcagg tcatgctgtt
    18961 tatgaccgcg tcagaagtga atacaacttc ccgatgctgg ctgcaaatgc catagaaata
    19021 aaaacaggag taccttattt caagccttat gccgttatcg aaaagaaggg aatgaaggtt
    19081 gtggtcttcg ggctaataac cccgtctgta ccgcgttggc tgccggaatc actctacagg
    19141 ggaataaggt tcgagaacat ggttgccact gccaggaagt ggatgcctgc gattcaggct
    19201 gaaaatcctg atctggtcat cgggttgttt cattcaggaa tgggtgacga gcgtgaaagg
    19261 ggagatgatg agaacagcac cctggcagtt gccatgaacg ttcccggatt tgatgttatc
    19321 ttctgcggcc atgatcacag gcaggatgtc agggaagtga cagacagcaa cggggataag
    19381 gttcttgttc tcgacggcgg cagccgggca gaggcactga tgagtgtcag cgtgtcattt
    19441 gacaggacag gcggagggag cagcacaccg gtcctggaag gcaggataat agagatgaac
    19501 accctgccgg tatcatccgg cttccttaag aagtaccgcg gtgcaatgga taaggtcagg
    19561 gaatatactt ccgaggtcat cggcaggtct gaaaccacag tgaccacgcg cgatgcctat
    19621 tttggtccat cggccttcat tgatctgata caccggatgc agcttgatat caccggtgct
    19681 cagatatcaa tggctgcccc gttgtcattt gaccagagta tctcaagggg agagctgagg
    19741 atcagggata tgtacaggct ttaccggttt gagaactttc tttataccgt gaggatgacg
    19801 ggaggagaga tagaccggca ccttgaacat tcagcgggtc aatggttccg gacgatggaa
    19861 aagggcagtg actatatgct taactaccgg acagatgaca acggcaaacc ggtcatcgtc
    19921 aacggaactg ccaggctgcg aaatccgtca tacaattttg attctgccat gggcatcagg
    19981 tacacagttg atgtgagcaa acccgtcggg gccagggtca atatcaccgc actcagtgac
    20041 gggtctgcgt tcagccacag tgccagctat acagtggcag tgaactcata ccgggccaac
    20101 gggggcggag gacattttca ggcagcaggc attgccggaa aggagctgga caggagaatt
    20161 atctccgcaa cggatcgaga tttgcggtac tacatgactg aatggatcag ggaaaaaggc
    20221 acaatatctc ccgttccgct cagccagtgg atgattgttc cggccggttg ggcagctgat
    20281 gcggaaaagc gggaaatgag aatgcttttt ggtaataatt gattattgtt tttatgaaga
    20341 tacttttaaa aattgcagct tttgtccttg ttgttctgac gcttacctca gccggcagtg
    20401 caaggcagaa gccacggaac ctggtactgt ttataggtga cggaatggga gtctcacatg
    20461 tctatgccgc catgacggtc tcaggcaaca cgatgacctt tccttccttc ccggtgaccg
    20521 gattcagcag gactcactca gccaacagtt attcaactga ctcggccgcc ggcggaacag
    20581 caattgcaac cggcgagaag accaataaca ggatggtggc gatgcgccct gacagcacaa
    20641 tactggttac acttcttgag catgcaaagg ctgccggaat gtctaccgga attgtctgca
    20701 ccagttcgtt aactgacgcc accccggctg attttgccac tcatgtcccc ctcaggtatg
    20761 aatatcgcgc tatagcgaaa cagtacatga acggggcggc tgacatattc atagggggag
    20821 gcaggaagta ttttgtcccg gtcccggatt caaccgggaa gatcaaccct gaggaagatg
    20881 tggtttcagg tctttcccgg gcaggttatg acgtaattta tgatcttgag tcgttcattg
    20941 cctcgaagtc aaatcggatt gcggggctga tggctgatga tgacatgccc aggttattcg
    21001 aggggagaga tcctcagtac cttgccaggg caacagcaaa ggctatcgag gtgctttcgc
    21061 gtaatcccag gggctttatc ctgatggtcg aaggttcgga gatcgatgat gccggccata
    21121 gcagaagcac gtcaatggtg actgatgagg tccttgatat ggacagggct gttgccgttg
    21181 cccatgattt tgcattgaga gatggcagga cactgattgt tgtgactgct gaccatgaga
    21241 caggaggcat gagcatcaca gggggagatc ttgcgaagag ggaggtaaaa gctgagttca
    21301 gcttcaccgg gcattcaggt gttatggtgc cggtgtttgc ttttggaccg ggagcaacgg
    21361 aattcacggg catacaggag aacaccggtc tcttccataa cttcttcagg cttctttccc
    21421 ttgacaggaa acgcagatga ggaagacgga gacagagagc agcgggcgga tgctccccct
    21481 ggtggaagag ttctactcgg tccagggaga ggggtgcaat acgggccggg cagcttactt
    21541 catacgggtg gggggatgcg acatatgctg cagctggtgt gataccaggt attcatggaa
    21601 tgccgaactc catccgatgg ttgccataga gagcattgcc ggaaacgttt ctgctgccgg
    21661 ggctgattcg gtggttgtta ccggcggcga gccgctgatg tggaacatgg aaccgctgtg
    21721 tgtcctgctc aggtcaaaag ggatcaaaac cttcctggag acctcggggg catatcccct
    21781 tacaggcaaa tgggactgga taaccctctc gccaaagaaa aattcaccgc ctctccccgg
    21841 aatctgggaa atggcggatg aactcaaggt ggttattcat gatggcactg attttgaaga
    21901 ggcagagagg tacagccgga atgtggggcc gggctgccag cttcttctgc agccggaatg
    21961 gagcatgtac agggagatga tagaggcgat aacggaatat gtgaaaaaga atacccggtg
    22021 gagggtctca ctgcaggcac ataaattcat gcatatcccc tgaatgagtg caggaagaac
    22081 aataatggcc cttatgttcc tggttctgac ccagtgttca gttaccggac agaaccttca
    22141 tacgcgttca aacagggcac tgaagtacta tgatcagggc aagagggatt acgaacttct
    22201 ttattacgac agggcagaga caaatcttct tcaggcgctg aaagaggatg gcaaattcta
    22261 tgaggctcac ctgattcttg gtcagctcta ttctgacacg ggtgactggg gaaaggcggt
    22321 gactcactac cgtgcggctg ttgccacgga ctcactcttc tttgtgccgg cactctacag
    22381 ccttggcagg gccgagttgc ggaccggaaa atactatgat gcttcaattc accttgacgc
    22441 ttttctgaaa cagcccggaa ccaccgccag gttgcgcgga gaagctgaaa agatgcttgc
    22501 cagctgccgt tttgccctct cgttcccggc atccctcttt gaaactgagc cggtcagcgc
    22561 cggtgactcg gtcaacactg tcctcgatga gtactggcct tcagtgacag gtgacggcca
    22621 gcagctgata ttcacccgag aggtgaaaag ggcggcaggc tatggcaggg actacaaccg
    22681 tgacaggcag gaagactttt atgtgagcag gctgactgca gacggatact ggggaacagc
    22741 ccggaatgcc ggagctccac tgaacactgc aggaaatgaa ggagcacagt caatctcttc
    22801 tgacggaagg tatatgttct ttacggcctg tgacagaaac gacggacagg ggagatgtga
    22861 catctacttc tcatcatatg acgg
//
LOCUS       DAOPXU010000040        22264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_2372_length_22264_cov_10.190328, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000040 DAOPXU010000000
VERSION     DAOPXU010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22264
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2372_length_22264_cov_10.190328"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PKK03_05745"
     CDS             <1..938
                     /locus_tag="PKK03_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNX83936.1"
                     /translation="RFLEDHKVPVVVTPMAKGVITYDHPSCAGVLFHALSDRLARLIR
                     AADLVIGLGYDPVEYNYESWLPEVPLVHFDTKPSDLRISGAIEVVSAPEEWFRLLSPL
                     GSSEEMMALAAQTRDEIRTCLNRAGRGLNPVTALSLMREVLPPDATVTADVGSHLHLT
                     GQMWDVQRGKLIMTNGWSSMGFGLPSAVAAALAGSGGPVVCVTGDGGFAMSAGEIITA
                     RRLGLKIIVVVFSDGELNLIKVKQSWKRLSPYGIHVHDGSLFGADRFLGVDVQRVTDL
                     SGMRSALKRALDSGGCGIIEAAVNPAVYDDLIVKS"
     gene            complement(947..1270)
                     /locus_tag="PKK03_05750"
     CDS             complement(947..1270)
                     /locus_tag="PKK03_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HNX83937.1"
                     /translation="MGKGDKKSKRGKIIIGSFGVRRRKKARKSIVAPSPKETAKAAVK
                     AAPVTTPEVPEAKPARTARPRTAAKAATKPAAKKSEKEAGAAAEKPAKAATKTKKPAA
                     KKEEE"
     gene            1413..>2038
                     /locus_tag="PKK03_05755"
     CDS             1413..>2038
                     /locus_tag="PKK03_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83938.1"
                     /translation="MATRKLWIYSPEVSPRLEYAAGVIFSTVLGINYEITTDRRKIGG
                     SPAVFYTNEKVKDQFVIRPSGLLDATGVEQIRPEVSFIGEMPVLFAAEGGSIPFDLFS
                     AVFYMLSRYEEYLPFSPDAHGRFPGAASLAGRGGFLRIPVVEIWSKFLAIELIRRYPV
                     LTIRHNEYSALVTVDVDQPFAYRSRGFLRSMGGLVKGLAGVGARPADR"
     assembly_gap    2039..2138
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2139..2872
                     /locus_tag="PKK03_05760"
     CDS             <2139..2872
                     /locus_tag="PKK03_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNX83939.1"
                     /translation="GGLVKGLAGVGARPADRIRTMTGELPDPYDSFGYIEEKLKESQS
                     EALFFFPTGDQGEYDHNPPFRDHDYGEIIRRYDRMYGSGLHSSYRSAGRIRTLKTEAE
                     RYSAITGHPPVKVRQHWLLLNMPDTYRDFREAGMTHDYTMGYHDEPGFRAGIARPFPF
                     YDLATERATTLTVVPFQVMDVTLRQYLHLQPEAALEVVRTLIAATRNAGGLFVSIWHN
                     TSLNEGNGWEGWRHVFEEMLFMQKV"
     gene            2869..3774
                     /locus_tag="PKK03_05765"
     CDS             2869..3774
                     /locus_tag="PKK03_05765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX83940.1"
                     /translation="MISYLRNNEVDRERWDACLNEIQAVKPYALSWYLDIMSPGWEAL
                     VDDDYYAVFPLPRQRKLGISYIATPLFLQQLGLFTADGDRKRMAEEFISFMPGFCRLV
                     DLCIGQEASVPGYRLTPKYNYELKLDDPYEKLWINYMTDCRRNINIARRYPQEIVEDP
                     TPGELIALFRTHTARRAGVTRESPYRRLRTLMEHCTSSGLGRILGVRSPKGKLLWGMF
                     VIETPGRTTMLFTAGSRKSRELRTSYLVIDHLIRSYAGTGQVIDFAGSSIPSIAIFMR
                     SFGGEKTTYWRLYRNSLPWPVKLLK"
     gene            complement(3775..4491)
                     /locus_tag="PKK03_05770"
     CDS             complement(3775..4491)
                     /locus_tag="PKK03_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX83941.1"
                     /translation="MEEITPLDTIIELKSCSIMQQDHLVLSDVNLKVDKGEFVYLVGR
                     VGSGKTSLIRTLNAQLPLAEGEGWVAGFSLHTIKKREIPLLRRKLGIVFQDFQLLTDR
                     SVHDNLEFALRATGWNNKVAVEERIKEVLEKVGLQNKGFKMPHQLSGGEQQRVVIARA
                     LLNDPEIILADEPTGNLDPETSEEIHILLKNISNTGRAVIMSTHKHSLVKKFPGRTLR
                     CENGKLSETEQNDQVIELLF"
     gene            4715..7762
                     /locus_tag="PKK03_05775"
     CDS             4715..7762
                     /locus_tag="PKK03_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX83942.1"
                     /translation="MNRKILTVVMVSLIAAGAAVKGQVSFSGNEVTSLLNNGYEMLSK
                     ARYTTAIGLFDKWLENGQNGTKLQRADAEYHAALASMRLMAPDAEFRMQRFITANPES
                     PMLNQARFESGRYCYQQRNYSCAIEWFEQTDRILLSDKEHAEYLFKLGYSQYRRGDTK
                     RAQMLFAELMDSETDYTAPATYYHAAIAYENGFYRTALEGFEKLKGDETFGSIVPFYI
                     TQIYYLDKNYDGILEMAPALIDQAGKKRETELYRFIGDAFFQKGNYSEAIPYLEKFIK
                     VTRLSDRNDKYELAYSYYQTGATDQATKLFNEVVGRSDILTQNAYYMLGSCYLQAGDK
                     KKAQMAFSAASKMSFDPDVQEEALFNFAKLAYENSYAPFGEGIDALHNYIRTYPNSEH
                     VSEAYDYLISAYMRLKNYQAALNSLEKISSKDDRLKEAYQKVAYFRGIEQFRNRQYAE
                     AVSMFDRSLTYKERNPDLYARALYWRGESNYRLGNNNGAVSDWENFRKLPAASSMDEY
                     ELTDYNLGYIWYNSGDYAKALPHFMAYVNRADQSERPDVVVDARNRIADCYYIRADYP
                     SAISYYDKVIDYGKVDADYAMFQKGFAQGLSNNNAAKITTLTGLVTKYPKSTYVPNAL
                     FERGRAYVATEQPSRGEADFTNIIAWYTGSQFVPPAMVQLGLIYYNAGENEKAVAQFK
                     KVIENYRNTPEARNAVNGLRNAYTDMDDVESFFAYMKNVEGLGDIEASTRDSMLYMSG
                     ENQYIKGNCERSSDIFRNYLKEFPSGSFATNARFYLADCLNRSGNVDEALDLYLRVVS
                     VGNNQFMEQSLLGAASITFGKKDYRGAWSLYERLGREASSPENRLYAYLGMMRSASAL
                     EDDDRVIASAELVLGSEKLTEDLAREATFLTAKANQRMGNNADALDDYRRVAQEVATA
                     YGAESKFRVAELLWIAGDIDGAEKEANQFVDMSSPHAYWTGKTFLLLSDIAIRKGDTF
                     QAKATLQSLIDYYTVRDDGIIEEARAKLAALTAAQPSGAGTTN"
     gene            7768..9489
                     /locus_tag="PKK03_05780"
     CDS             7768..9489
                     /locus_tag="PKK03_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83943.1"
                     /translation="MVLNNEMTKKMKGKYYIMAVALLISVTAAAQTEDETIKRTVTLY
                     NPYKPTLHEATKRALLPSDDDTTTVNLRFNYDFTPGSFVPEYEISPIKSAVLSPEPLP
                     DLKKGYVSLGLGTYLSPFLEVSISNGRSGKGAVGLFTRTYGSAGRIELENDRRVYAGF
                     LDNQALLFGKKYFRRGRLDSDIDFRQMTRHAYGYNPDVTGYDPDRKDIRSLYYDVTGK
                     VRYFTMEPDSSDLNWDAALKYNFFTRQGDGLQHNPGLSLSAGTNMFGLYAGIDAGYDM
                     YIFSKALDTKSRNLITLAPYITRGNDDWRFRFGLKVAADVKENHDPFVGGVRKLYMFL
                     YPDAMFTFRIIPKFLRFTAAIDGFMENNQARNTAYVNPWMLPGDTLYNLRSTDNELRI
                     SAGLSGTMNVNASYSLGASYTLFKDMLLFMNDTIGYGNYFVPVYSDGSLLKVNGEVSV
                     PLNRQLTLSLLGNYYGYNLSGQEYAWHKPEWDGSLKAAYNLRNKIIASADLTLTGVRY
                     ARVMAPEKTVKLPFHPNLNMGIEYRYTPEISFWVKCNNISYSRYFEWNYYPSRNLMLL
                     GGISYSL"
     gene            9645..11627
                     /gene="gyrB"
                     /locus_tag="PKK03_05785"
     CDS             9645..11627
                     /gene="gyrB"
                     /locus_tag="PKK03_05785"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNX83944.1"
                     /translation="MTNEELRKRSREQDEYSADSIQVLEGLEAVRKRPAMYIGDVGTR
                     GLHHLVYEVVDNSIDEALAGFCTRIDVTINADNSITVVDNGRGIPVEIHEKEKRSALE
                     VVMTVLHAGGKFDKDSYKVSGGLHGVGVSCVNALSSRMTVEVMRQGKRWSQTYERGKP
                     VAPVAAIGESGESGTITTFMPDNTIFQTIEFNFDVLAARLRELAFLNKGILLTLTDKR
                     ENAENGGSGEQRSDTANFYSERGLIEFVEYLDVNRERLTEKVIYINYDKGEVPVELAL
                     QYNTSFSENVHSYVNNINTIEGGTHLAGFRRGLTRTLKKYAEDSGLTSKLKFDINGDD
                     FREGLTSVISVKVAEPQFEGQTKTKLGNSEVMGAVDVAVSTALTNYLEENPKDARSIV
                     QKVILAATARHAARKARELVQRKNVLSGSGLPGKLADCADRDPSVSEIFLVEGDSAGG
                     TAKQGRDRRFQAILPLRGKILNVEKAMQHKIFDSEEIKNIFTALGVNIGTEDDSKALN
                     TSGLRYAKVIIMTDADVDGSHITTLIMTFFFRHMKELIENGHIYIATPPLYLVRRGKS
                     EKYCWSEEERDAAVRELGQGKESAVSVQRYKGLGEMNAEQLWDTTMNPETRTLRQVTI
                     DSAAEADHIFSMLMGDDVPPRREFIERHAKYANIDA"
     gene            11795..13243
                     /locus_tag="PKK03_05790"
     CDS             11795..13243
                     /locus_tag="PKK03_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNX83945.1"
                     /translation="MSQNGQPSFRKSLNLVDSSALIIGSMIGSGIFIVSADMSRTLGS
                     PGWLLVAWLITGFMTIMGALNFGELAAMMPKAGGTYVYLREAYNPLTGFLYGWTLFMV
                     IQTGTIAAVAMAFGKFFGVIVPWINEGNILLNLGPVRFSSVHVVAIGSLALLTWINSR
                     GIRTGKNVQNTFTFTKVIILMLLLLAGFIFAGSDGFRANSEYFWEAAKLDRVTGIVTP
                     ITGGMIVVAIAMAMVGSLFTCDAWTNVTFASAEVVDPRRTLPRSLFWGTLIAFTLFFL
                     SNIVYVKVLPLRGMPDGAGVLERGMAFATNDRLGTAAISSIFGDYAAIIMAVLIVIST
                     FGCNNGLILSGARVYYAMAADKLFFRNAGKLNARAVPGNGLWIQFAWASLLCLSGSYS
                     QLLDYVVFAVLLFYVLTILAIFVLRHRRPEADRPYRAFGYPVITALYILMAATIMIIL
                     LIYKPAFTWPGLIIVLIGIPVYYIWKRFNKTI"
     gene            13245..13874
                     /locus_tag="PKK03_05795"
     CDS             13245..13874
                     /locus_tag="PKK03_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNX83946.1"
                     /translation="MEIFQTVFDWYMANLNYFTIYLLMAIESSFIPFPSEIVVPFAAW
                     KAGEGTLTMWGVILSSTAGAMTGAVINYYLAMWIGRPVLYRLADTRWAHLLLITRESV
                     EKSEKYFVKHGKASTFVGRLVPAVRQLISLPAGLARMNIGHFLLYTFLGATLWNMILA
                     VIGYYAYDKREHILPYIDWIMYAVGAIFVLWLSWKGYQLYRNRKKTSIQ"
     gene            complement(13864..15150)
                     /locus_tag="PKK03_05800"
     CDS             complement(13864..15150)
                     /locus_tag="PKK03_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX83947.1"
                     /translation="MTKPSLSIMILLLLLPLAAGAQRTMSLDEAIMQALENNYSLKIS
                     RNDEAIADNNVSLSPFLPSLTGTGRQSQTTNNARSSSSGEDKTRTNLYTAGVALNWRL
                     FDGFGMFTDYSRQQELLAVSSQRVKINVENLIMRVCSEYYNIIVQQNRMQSALTSLAL
                     SRSRYENAEEKYLLGVISGLDLQQARIDLNADSSAVLSQQETVISAYIRMTNLLNAGH
                     EINFNINDTIILAPEIDVDSLRERTLSFNNQLILARQGEKLSEYDIESVRAERYPTVN
                     FSTGYNLTRNEFPWQASSYNQTNGLNWGFNLSWNIFSGLEINRRMANAKIEAESSRLA
                     YLDIENEILGELDLLYNTYRNNIIVTNFETQSAEVARASLEIAMERYRLGSLSGLEFR
                     EYQRNYLNAINRRLTALYQAKISEIGLRLLSGELTE"
     gene            complement(15131..18190)
                     /locus_tag="PKK03_05805"
     CDS             complement(15131..18190)
                     /locus_tag="PKK03_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX83948.1"
                     /translation="MSLSSLSIKRPVLATVMALVILIMGGIGYTFLGVRDYPSVDPAI
                     VTVSTSFPGANSDVIETQITEPLESAINSVQGIRSISSSSSDGSSRITVEFKLEVDME
                     TAVNDVRDKVSGAMRRLPQDVDPPVVQKADADAQPIFAVTLRSDTRNIIDVSQYAELN
                     FRERLQTISGVSAVSVWGSKKLAIRMKMDPARLAAYGLTPLDVRNAVTRENVELPSGR
                     IEGDNTELTIRTLGRLRTVDEFNDLIITKNGDRVVKFSDIGTAEIDAENIRSHLLSNG
                     KPSVNVVLVPQPGANYIDIVDNAQILLKQLEKDLPEDITMEVLFDNTLYIRESIKEVK
                     NTIFVAFLLVVLVIFLFLRNWRTTLIPVVAIPVALIGAFFVMYLAGFTINILTLFAIV
                     LSIGLVVDDAIVVVENIYTKVEHGMKPMDAALTGSREIYFAIIATTIALISVFFPIVF
                     LQGVTGRLFREFSLVIVGAVIISAFLALSLTPMISSRVLKHNAMEGRFYRATEPFFSG
                     MINFYRKAITSFLKVRYWALIIIVVAGGLIAWMWTLLPSEMAPLEDRGYVNISARATE
                     GASYEYMSAYMDQIAPVVKEQVPESDNIMMMVGMGSVNSANMRVRLVGQEDRKRSQQE
                     IATALSPVLRQYTGAQTIVSQQQTFGARRGGLPVQYVIQAKNLNDLKRVIPDFLAMTS
                     ESPYFSASDVNLKFTKPELTVLINREKASMMGINVQNIAQTLQLTMSEQRIGYYILDG
                     KQYQIFSQFDKAMRNDPFDLTSTYVRNDKGQLIQLDNIVEIRENSLPPQLYRYNRFVS
                     ATVSAGLEGRYTLGEGIEEMDRIADRVLDENFSTTLSGDSKDFVESTSSLLFAFVLAL
                     VLIYLVLSAQFESFRDPIIILVTVPLALIGALFTMVIFGQTLNIFSQIGLIMLIGMVA
                     KNGILIVEFANQRQQAGLSVMDAVREASVARFRPILMTSLTTILGIMPLALATGAGSE
                     SRITMGVTVVGGMFFATFLTLFVVPSVYSYLAGKKNAENSINDQTQS"
     gene            complement(18228..19436)
                     /locus_tag="PKK03_05810"
     CDS             complement(18228..19436)
                     /locus_tag="PKK03_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX83949.1"
                     /translation="MKKGTKIALWISIPLVLLIWFAGPRLGLWGNRDLKKKGEVPAAQ
                     VQATGQGPGGQQGQGPGGQQGQTQGGQQGQTQGGQKGQGQRPGGQGQLPVTGTIAKPS
                     YLTNGIRSAGSLLANEEVDIVSKVSGKVTAVYFKEGSKVKKGDLLVKIYDEDLQAQLR
                     RSEIQEKMLSEKLERQRVLLSKDAVSREAFDQLQTDYNVILADINLLKVRIAETEVRA
                     PFDGVIGFRYVSEGTYVTPQVKIAHLFDQSILKLEFAINEKYVSENLMGKKISFKTEG
                     YRDEFFATVYAVDYRIDQTTRTIGLRARYDNRTGKLVPGMYASLTLITDEKMNALQVP
                     TEAIVPEMNEKKIWVARNGRAELIPVVTGARTASAIEVISGLAAGDTVLTTGLIQLRP
                     NMAVRVNITE"
     gene            complement(19562..>20701)
                     /gene="dacB"
                     /locus_tag="PKK03_05815"
     CDS             complement(19562..>20701)
                     /gene="dacB"
                     /locus_tag="PKK03_05815"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNX83950.1"
                     /translation="KKGILEGDVVIIGGGDPALGSEYFSEYYGDVTAAWVSALKAAGV
                     KKVRGRVAAGESIYDFNPAPAGWAWGDLGQYYGAGVYDININNNEYKIYISGRAEGEA
                     AVIDSVQDYGKDIILDSYLLSSGSTDRGYVYNAPYSPMAWVTGSVPADSSFTLRASIP
                     DPPLALVKKLDAAMRKAGIVVDGQPVRDRVSAEGKKRFSVATTLSPALAEIVKVTNHE
                     SVNMYAEALRKHLGWEIRGEGTWNAGSAVIRQFLDSIGCEPYEAVILDGSGLSSNNNI
                     SALMTVRLLVHMYNSSVSEAFISSLPMGAVSGTMKNYFRDEMFKGRVVAKTGTITSAK
                     SFAGYFTTNSGRKIAFTTFANGFTVPSRKITDNMERVVREIMIKY"
     assembly_gap    20702..20801
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20802..21820)
                     /gene="rlmN"
                     /locus_tag="PKK03_05820"
     CDS             complement(<20802..21820)
                     /gene="rlmN"
                     /locus_tag="PKK03_05820"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNX83951.1"
                     /translation="MMEKKKLFGLTLNELSEVVKDASLPPYAAKQIAGWLYRREAPHI
                     SAMTDLSLAARGILEKDYETGLNPPVSESSASDGTKKYLFRVSENRFIETAFIPDRDR
                     ATICLSVQSGCRMGCRFCMTARQGFQGNLSSNEILNQFRSLPEYDSLTNIVFMGMGEP
                     LDNLEEVLRSLEILTSGWGYGWSPTRITVSTIGITGRIEEFLGKSKCHLAVSLHSPFD
                     SERQSLMPAQKAHPVKEVLDTIRGFDFGRQRRVSFEYIVFRGLNDTPAHVRELARILN
                     GIRCRINLIRFHSIPGSEFSSPDQRGMEAFRDSLNAKGIITTIRASRGQDINAACGLL
                     STLEQ"
     gene            complement(21929..>22264)
                     /locus_tag="PKK03_05825"
     CDS             complement(21929..>22264)
                     /locus_tag="PKK03_05825"
                     /inference="COORDINATES: protein motif:HMM:NF013813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNX83952.1"
                     /translation="TDMVSFGRALRWSPAYAPEGVNVNLASINNGRIRVRTYERGVED
                     ETLACGTGITASAIAAAYTGKIVTPAAEVEARGGMLSVRFDLTGEKATGIYLTGPATF
                     VFSGEKEIQ"
BASE COUNT         5122 a         5457 c         5932 g         5553 t
ORIGIN      
        1 tcagattcct ggaagatcac aaagttccgg tagtggtcac cccgatggca aagggagtca
       61 tcacatatga tcatccatcc tgtgccggag tcctgttcca tgcgctgagt gaccggcttg
      121 caaggctgat cagggcagcc gacctggtta tcggactggg ttatgatccg gtggagtaca
      181 attatgaatc atggctcccg gaggtaccac tcgttcattt tgacacaaag ccttctgacc
      241 tgcggattag cggagcaatt gaggttgtgt cagctccgga ggagtggttc aggttattgt
      301 cacccctggg aagctctgaa gagatgatgg ccctggcagc ccagacaagg gatgagatac
      361 ggacatgcct gaacagggca gggaggggat tgaatcctgt aaccgccctg tcgttgatga
      421 gagaagttct ccccccggat gccaccgtta cggccgatgt gggatctcac ctgcacctta
      481 caggccagat gtgggatgtg cagcgcggaa agctgataat gacaaacggg tggtcatcga
      541 tgggattcgg acttccttct gctgttgcag ctgccctggc agggagcggc gggccggtgg
      601 tgtgcgttac cggtgatggc gggtttgcga tgtctgccgg tgagataatt acagcacgcc
      661 ggctggggct taaaatcata gttgtcgtct tcagtgacgg ggagcttaac ctcatcaagg
      721 ttaaacagtc atggaagcgc ctgagtccat acggcattca tgtacatgac gggtccctat
      781 tcggagctga cagattcctg ggagttgatg tgcaacgtgt cactgacctt tccggaatgc
      841 gttcagcact taaacgtgcg cttgactccg ggggttgtgg aataatcgag gcagccgtca
      901 atccggcagt ctatgatgat cttatagtaa agagctgaaa tacagactat tcctcttctt
      961 ttttagcagc gggctttttt gttttggttg cagcctttgc aggcttttca gcagcggctc
     1021 ctgcttcttt ttcactcttc ttagctgcag gtttcgtggc tgcctttgca gctgtcctgg
     1081 gcctggctgt cctggcaggt tttgcttccg gtacctccgg tgtcgttaca ggggctgctt
     1141 tcactgctgc ctttgctgtc tccttcggag atggtgcaac aatgctcttc ctggctttct
     1201 tacgacggcg gacgccaaag gatcctataa taatctttcc tctctttgat tttttgtcac
     1261 ctttgcccat attttttgaa attttaaatt gtgaacacaa aattacgcaa acaacaaaag
     1321 tagtaaaatt tagtcccggg gcatcaaatt ctgttaataa aaaggatcaa cactcgggga
     1381 cggtaaccgg ccgttttatt aaatttgctg atatggcaac caggaagcta tggatttact
     1441 ctccggaagt ctctccccgg cttgagtatg cggcaggtgt gatcttcagc accgttctgg
     1501 gcataaatta tgagataaca actgacagaa ggaagatagg cggcagtcct gctgtcttct
     1561 acaccaatga gaaggtgaag gatcagtttg ttatccgtcc gtcgggactc cttgatgcga
     1621 caggtgttga acagataagg cctgaggtgt catttatcgg agagatgccg gttttgtttg
     1681 cagctgaagg gggaagcatc ccgtttgatc ttttctccgc cgtattttac atgctgtcga
     1741 ggtatgagga atatctccct ttctcacctg atgcccacgg acggtttccg ggtgccgctt
     1801 ccctggccgg gaggggaggg ttcctgagga ttcctgttgt ggagatctgg tcaaaattcc
     1861 tggcgattga actgataaga cggtatccgg ttcttaccat aaggcacaat gagtacagtg
     1921 ccctggttac tgtggatgtt gaccagcctt ttgcataccg cagcagggga tttctgagga
     1981 gcatgggcgg tctggtgaaa gggctcgccg gggtgggagc caggcctgct gaccggatnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg ggcggtctgg tgaaagggct
     2161 cgccggggtg ggagccaggc ctgctgaccg gataaggacc atgaccggag agctaccaga
     2221 cccgtatgac agcttcggat acattgaaga aaagctgaag gaaagccaat ccgaagccct
     2281 ctttttcttc cccacgggtg accagggaga gtatgaccat aatcctccct tccgtgatca
     2341 tgattacggt gaaattatcc gcaggtatga ccggatgtac ggcagcggcc ttcattcatc
     2401 ctacaggtcc gcgggcagga tcaggactct gaagactgag gcggaacggt acagcgccat
     2461 taccgggcat ccccccgtga aggtgcgtca acactggctg cttcttaata tgccggacac
     2521 ataccgtgac ttccgtgagg cgggcatgac gcatgactat acaatgggtt atcatgatga
     2581 gcctggtttc agggcaggca tcgcccggcc cttccccttc tatgatcttg ccaccgagag
     2641 ggcaaccacc cttactgtag tgcctttcca ggtgatggac gtgactctca ggcagtatct
     2701 gcatcttcag cctgaggctg ccctggaggt tgtcaggact ctcatcgccg cgaccaggaa
     2761 cgcaggcggc ctgtttgttt caatatggca caacacctcc ctcaatgaag gcaacggatg
     2821 ggagggctgg agacatgtat ttgaggaaat gctgttcatg cagaaggtat gatcagctat
     2881 ctgagaaaca atgaggtaga ccgtgagcgg tgggatgcgt gcctgaatga gatccaggcc
     2941 gtaaaacctt atgccctttc ctggtatctg gatataatgt caccggggtg ggaggcactt
     3001 gtagacgatg attattatgc ggtttttcct ctgccgcgcc agagaaaact ggggatcagc
     3061 tatattgcca cccccttgtt cctgcaacaa ctgggactct tcaccgctga cggtgaccgg
     3121 aaacggatgg ctgaagagtt catctcgttc atgcccggtt tctgcaggct ggttgatctc
     3181 tgcataggac aggaggcctc ggtgccgggc tacaggctta caccgaagta caattatgag
     3241 ctgaagctcg atgatcctta tgaaaagttg tggattaatt acatgactga ctgccggagg
     3301 aacatcaaca ttgcacgtcg ctatccccag gaaatagttg aggatccaac ccccggagag
     3361 ctgattgcgc tcttccgtac ccatactgcc aggagggcag gtgtgacaag ggaatcacca
     3421 taccggagac tccggaccct gatggagcat tgcacctcat cgggactcgg aaggattctg
     3481 ggtgtaagat cgccaaaggg aaaactcctc tggggcatgt tcgtgattga aactcccggg
     3541 cgcacaacaa tgctttttac ggcgggaagc aggaaaagcc gcgaactgcg aacatcctac
     3601 ctcgtcatag atcacctgat caggagttat gcaggtaccg gacaggtaat tgattttgcc
     3661 gggtcgtcaa ttccgtcaat agctatcttc atgagatcat tcggagggga gaaaacaacc
     3721 tactggaggc tgtaccgcaa cagtctcccc tggcccgtga agcttctgaa atgatcagaa
     3781 gaggagttct attacctgat cgttctgctc tgtctctgaa agcttgccgt tttcgcaccg
     3841 gagggtcctt ccgggaaatt ttttgaccag tgagtgcttg tgggttgaca taataacggc
     3901 ccggcccgta ttgctgatgt ttttcagcag gatatgtatc tcctctgatg tttccggatc
     3961 aaggttgcca gtcggttcat cggccagtat tatctccggg tcattcagca gggcacgtgc
     4021 aatgaccacc ctctgctgtt cgccgccaga gagctggtga ggcattttga agcccttgtt
     4081 ctgcaacccg accttttcaa gtacctcttt tatccgttct tcaaccgcaa ccttgttgtt
     4141 ccagcctgtc gctctgaggg caaattccag gttgtcatgc actgacctgt cggtcaggag
     4201 ctggaaatcc tggaacacta ttccgagttt tcttctcagc agaggtatct ccctcttttt
     4261 tatcgtgtgg aggctgaatc ctgccaccca tccttcgcct tcggcgagag gaagctgtgc
     4321 attgagggtg cggatgagtg aagtcttgcc gcttcccacc ctgcctacca ggtagacaaa
     4381 ctccccttta tcgactttca ggttcacatc gctgaggacg agatgatcct gctgcatgat
     4441 cgaacagctt ttcagttcaa tgattgtgtc aagaggggtg atctcttcca ttcggaacgg
     4501 tttttgtgca aaataaaaaa gaattaacaa tcagcagtct ctgtgttgat aaaatgaagg
     4561 ttaaggaaga tttcgcgagg cattataatt gtatttttac ggctaatgaa actgcgccgg
     4621 aaagtctgcg caatatattg ttaatgaatt aattaattgc aggaacagcc tgcaggtgaa
     4681 tgtttttgag acagcttgag aaaaggataa agttatgaac aggaagatac tgacggtggt
     4741 catggtctca ctcattgccg caggagcagc ggttaagggg caggtatcat tttcagggaa
     4801 tgaggtgacc tccctgctga acaacggtta tgagatgctt tcaaaggcca ggtataccac
     4861 ggccatcggg ttgtttgaca aatggcttga gaacgggcag aacggtacca aacttcagag
     4921 ggcagatgcg gagtatcacg ctgcgctggc atcgatgagg ctgatggctc ctgatgccga
     4981 gttcaggatg cagcggttca tcaccgccaa cccggagagc ccgatgctca accaggcgcg
     5041 gtttgaatcg ggccgctatt gttaccagca gcggaattat agctgcgcca ttgagtggtt
     5101 tgaacagact gaccgcattc tgctgagtga caaggagcat gccgagtatc ttttcaagct
     5161 tgggtactcg cagtaccgga ggggtgacac caagagggca cagatgctct tcgccgagct
     5221 gatggattcc gaaaccgatt ataccgcgcc ggccacttat taccacgccg caattgccta
     5281 tgagaacgga ttctaccgta cggcactgga gggttttgag aaactgaagg gtgatgaaac
     5341 cttcggaagc attgttccat tctacattac ccagatctat tacctcgaca agaactacga
     5401 cggtatcctg gagatggctc ctgcacttat tgaccaggcg ggaaagaaaa gggagacaga
     5461 gctttaccgg ttcatcggtg acgcattttt tcagaagggc aattacagcg aagccatccc
     5521 ttaccttgaa aaatttatca aagtgaccag gctcagtgac cgcaatgata aatatgagct
     5581 ggcatacagc tactatcaga ccggggccac ggatcaggcc acgaagcttt tcaacgaagt
     5641 ggttggccgc agtgacatcc tgacgcagaa cgcgtattac atgctgggtt cgtgctacct
     5701 tcaggcagga gacaaaaaaa aggcgcagat ggctttctcg gccgcctcaa agatgagttt
     5761 tgacccggat gtacaggagg aggccctctt caacttcgca aaactggcat atgagaattc
     5821 atacgctccc tttggggagg ggatagatgc actgcataac tacatccgca catatcccaa
     5881 ttcagaacat gtgtcagagg cgtatgacta cctgatatcc gcctacatga gactgaagaa
     5941 ctaccaggcc gccctgaact cacttgaaaa gataagcagc aaggatgacc gtctgaagga
     6001 ggcgtaccag aaggtggcct atttccgtgg catagagcag ttccgcaaca ggcaatatgc
     6061 cgaagcggta tcaatgttcg acaggtcgct cacctacaag gaaaggaatc ctgaccttta
     6121 tgcccgggcc ctgtactgga gaggggagtc taactaccgt ctcggcaaca ataacggagc
     6181 agtcagtgac tgggagaact tcaggaagct gcctgccgct tcatcgatgg atgagtatga
     6241 gctgacggac tataacctgg ggtacatatg gtacaattca ggagactacg cgaaagcact
     6301 gcctcatttc atggcatacg tcaacagggc tgatcagagt gagagacctg acgtggttgt
     6361 tgatgcccgc aaccggatag ccgactgcta ctacatcagg gctgattacc cgtcggcgat
     6421 atcgtactat gacaaggtaa ttgactatgg caaggtcgat gctgactatg caatgttcca
     6481 gaagggattt gcccaggggt tgagcaataa taacgcagcc aagatcacca ccctcacagg
     6541 cctggttaca aagtacccga aatcaacata cgtgccgaac gcactgtttg aaaggggaag
     6601 ggcatatgtt gccacggagc agccttcacg cggtgaagcc gactttacga atatcattgc
     6661 ctggtacacc gggagccagt tcgttcctcc tgccatggtg cagctcgggc ttatctatta
     6721 caatgccggg gagaatgaga aggctgttgc ccagttcaag aaggtaatag aaaactacag
     6781 gaacaccccg gaggcccgca acgctgtcaa cggtctcagg aacgcttata ctgacatgga
     6841 cgatgttgag tcgttctttg catacatgaa gaatgtcgag gggctgggtg acatcgaggc
     6901 ttccacgcgt gactccatgc tgtacatgtc cggggagaac cagtacataa agggcaactg
     6961 tgagagatca tcagatatat tcaggaacta cctgaaggag ttcccgtcag gcagctttgc
     7021 caccaatgcc cggttctacc ttgctgactg ccttaaccgg tcaggcaatg ttgacgaagc
     7081 gcttgacctc tatctcaggg tggtcagtgt agggaacaac cagttcatgg agcagtcgtt
     7141 gctcggcgca gcctccatca cattcgggaa gaaggattac agaggcgcat ggtcgcttta
     7201 cgaacggctg ggcagggagg cttcaagccc ggagaacagg ctgtatgcct atcttggaat
     7261 gatgcgttca gcgtcggctc tcgaggatga tgacagggtg attgcctcgg cagagctggt
     7321 gctcggttcg gagaagctga cggaggatct ggccagggag gccaccttcc tgacggcgaa
     7381 ggccaaccag agaatgggta acaatgccga tgcactcgat gactatcggc gtgtggccca
     7441 ggaagttgcc acagcctacg gggcagagtc gaagtttcgt gtggctgagt tgctgtggat
     7501 tgccggagac attgacggtg ccgagaagga ggctaaccag tttgtcgaca tgagttcgcc
     7561 tcatgcatac tggaccggca agaccttcct gctgctgtca gacattgcca tcaggaaggg
     7621 agacaccttc caggcgaagg caacgctgca gagcctgatt gactattata ctgtcaggga
     7681 cgacggcata attgaagagg ccagggcaaa acttgccgcc ctgacagccg cacagccttc
     7741 aggcgccgga acaactaact gaatcagatg gtactgaaca acgagatgac gaagaagatg
     7801 aaaggaaaat actatatcat ggcagttgcc ctgctgatat cggtgacagc cgccgcgcag
     7861 acagaggatg agacaataaa gagaacagtc actctttaca acccgtataa gcccaccctt
     7921 catgaggcca ccaagagagc cctcctccct tcggatgacg acaccaccac ggttaacctg
     7981 cggttcaact acgactttac gcccggttca ttcgttcctg aatacgagat cagccccata
     8041 aagtcggcgg tactctcgcc agagcctctg ccggacctga agaagggtta tgtaagcctc
     8101 ggtcttggca cctatctgtc gccttttctt gaggtgagta tctcgaacgg caggtccggc
     8161 aagggcgctg tcgggctctt cacccggacc tatgggtcgg caggcaggat agagcttgag
     8221 aatgacagga gggtatatgc aggattcctg gacaaccagg ctctgctgtt cgggaagaag
     8281 tatttcagga ggggcaggct cgacagtgac attgatttca ggcagatgac acgtcatgcc
     8341 tacgggtaca atccagatgt taccggttac gaccctgaca ggaaggatat caggtcccta
     8401 tactatgatg ttacagggaa agtcaggtac ttcaccatgg agcctgattc aagtgatctg
     8461 aactgggatg ccgcgcttaa gtacaacttt ttcaccaggc agggtgacgg tctgcaacat
     8521 aacccgggac tctcactcag tgccggaaca aacatgttcg gactttatgc aggtattgac
     8581 gccggttatg acatgtatat cttttcaaag gctctggata caaagtcaag gaaccttatt
     8641 accctggctc cgtacatcac cagaggcaat gatgactgga gattcaggtt cgggctgaag
     8701 gtggctgccg atgttaagga gaaccatgat ccctttgtcg gaggtgttcg caagctttat
     8761 atgtttttat atcctgatgc catgttcacc ttcaggatca tccccaaatt cctcaggttc
     8821 actgcagcca ttgacggttt catggaaaac aaccaggcgc ggaatactgc ctatgtcaat
     8881 ccatggatgc ttccgggtga caccctgtac aacctgcgca gcaccgacaa tgagctgagg
     8941 atctccgcgg ggctttcagg cacaatgaat gtcaatgcat cctactccct cggtgccagt
     9001 tataccctct tcaaggatat gcttctgttc atgaatgaca ccatcgggta tggcaactat
     9061 tttgtgcctg tgtattcaga cggcagcctg ttgaaggtaa acggtgaggt gtcagtgccc
     9121 ctcaaccgcc agctgaccct ttcactgctt ggcaactact acggatacaa cctgtcggga
     9181 caggagtatg cctggcacaa gcctgagtgg gacgggtcac tgaaggctgc ctataatctc
     9241 aggaacaaga tcatcgcttc tgccgatctc accctgaccg gtgtccggta tgcgagggtg
     9301 atggcgccgg aaaagacggt aaaactgccg ttccacccaa acctgaatat gggcatagaa
     9361 tacaggtaca ctcctgaaat ctcattctgg gtcaagtgca ataatatatc ctacagccga
     9421 tattttgaat ggaactatta cccgtcaagg aacctgatgc ttctgggcgg aatttcatac
     9481 agtctgtaaa gtgccggaaa ccgggatttt ttgttcatat ttttccatta ataattgcct
     9541 tttttcgaag gatagccggc atattttgct acctttgtat gattttataa aaaacatatt
     9601 atcaaactgt taatcagtta taaattcaat ttaaaactac ttgtatgact aatgaggaac
     9661 tgaggaagag gagcagggaa caggatgagt attcagcaga cagtatccag gttcttgaag
     9721 gactggaagc cgtaaggaag cggccagcga tgtacatcgg ggatgtaggg acgagggggt
     9781 tgcatcatct tgtatatgag gttgttgaca attccataga tgaggccctc gcaggattct
     9841 gcacccgtat cgatgtgaca atcaacgcag ataattccat aaccgttgtg gataatggcc
     9901 gcgggattcc cgtggagata catgagaagg agaagcggtc agcccttgaa gtggtcatga
     9961 ccgtgctgca tgcagggggt aagtttgaca aggattccta taaggtttcc ggcggtctcc
    10021 atggtgtagg cgtctcctgt gtcaacgcac tctcatcgag gatgaccgtg gaggtcatga
    10081 gacagggcaa gaggtggtca cagacttatg agcggggaaa gcctgttgca ccggtagcag
    10141 cgataggtga atccggggag tcaggcacca taaccacatt catgcctgac aacacaatat
    10201 tccagacaat tgagttcaac ttcgacgtac ttgcagcccg cctcagggag ctggctttcc
    10261 tgaataaagg cattctcctt acactgaccg acaagcgtga gaacgctgaa aacgggggca
    10321 gcggtgaaca gcgttcagat accgcgaatt tttattccga gagagggttg attgagtttg
    10381 ttgaatacct tgacgtaaac agggagaggc ttacggagaa ggtgatttat atcaactatg
    10441 acaagggtga ggtacctgtt gaactggccc ttcaatacaa tacatcattc tctgaaaacg
    10501 tgcactcata tgtgaacaac attaacacaa ttgagggagg aactcatctt gcaggtttcc
    10561 ggaggggttt aacccgcacg ctgaagaaat atgccgagga ttccgggctg accagcaagc
    10621 tgaagtttga catcaacggt gatgatttcc gtgaaggact gacctctgtc atctccgtga
    10681 aggtagctga gccccagttt gagggtcaga cgaagaccaa actgggtaac tccgaggtga
    10741 tgggtgccgt ggacgtggca gtaagcactg cccttaccaa ctaccttgag gagaatccaa
    10801 aggatgccag gtcaatagtg cagaaggtga ttctcgctgc cacggcacgt catgcagccc
    10861 gaaaggcgcg tgagctggta cagagaaaaa atgtgctttc aggctcaggt ctgcctggca
    10921 agctggccga ctgtgcagac cgtgatcctt cggtgtcgga gatatttctt gtggagggcg
    10981 attctgcagg cggaacggca aaacagggcc gtgaccgccg ttttcaggcc atccttcccc
    11041 tccggggcaa gatcctcaat gttgagaagg caatgcagca taagatcttt gacagtgagg
    11101 agatcaagaa catattcacg gccctgggtg tcaacatagg caccgaggat gacagcaagg
    11161 cattgaacac ttcgggcctc agatacgcca aggtaattat catgaccgat gccgatgttg
    11221 acggctcaca tatcaccaca ctgataatga ccttcttctt caggcatatg aaggagctga
    11281 ttgaaaacgg gcatatttac attgccactc cgccgctata ccttgtcagg aggggcaaga
    11341 gcgagaagta ctgctggtca gaggaggagc gtgatgctgc cgtcagggaa ctgggccagg
    11401 gtaaggaatc ggctgtgtct gtgcagaggt acaagggtct cggtgagatg aatgcagagc
    11461 agctatggga taccacaatg aatccggaga caagaaccct ccgccaggtt accatagaca
    11521 gcgccgcgga ggcagaccat atcttctcaa tgcttatggg tgacgatgtg ccgccaagga
    11581 gggagttcat agagaggcat gcgaagtatg ccaatattga tgcatagaca ggcaggagac
    11641 agcatttccc gtagggctta aagcattgca gatttgcgat ttgcgattga ggatattccc
    11701 cgtaggggat caagcattgt agcagagatg tgattattaa tattccccgc aggggatgaa
    11761 gcattgtccc ctgcggggtc atacataatt gtacatgtca cagaacggcc agccatcctt
    11821 caggaagagt cttaacctgg tagactcatc cgcattgatc atcggctcga tgataggttc
    11881 cgggatcttt attgtcagcg cagacatgtc gcgcaccctc ggttcgccgg gatggctgct
    11941 ggtggcctgg ctgatcacag gcttcatgac catcatggga gctctgaatt tcggtgaatt
    12001 agcggccatg atgcctaaag ccggaggtac atatgtctac ctgcgcgaag cgtataaccc
    12061 gcttaccggc ttcctttatg gctggaccct cttcatggtc atccagacag ggacgatagc
    12121 agccgtggcg atggctttcg gcaagttttt cggtgtgatt gttccatgga taaatgaagg
    12181 caatatcctt ctgaatctcg ggccggtcag gttctcatcg gtacacgttg ttgccatagg
    12241 gtctctggca ttgctgacat ggatcaactc gagaggaatc aggacaggca agaacgtgca
    12301 gaacaccttc accttcacca aggtgatcat cctgatgctt ctgttgctgg ccggttttat
    12361 ctttgccggt tctgacggtt tcagggcgaa ctcggagtac ttctgggaag ccgcgaagct
    12421 cgacagggtt acaggcattg taacgccgat caccggaggc atgatagtgg ttgccattgc
    12481 catggcgatg gttggctcgc tgttcacatg tgatgcatgg accaacgtga cattcgcatc
    12541 ggcggaggtg gttgatccca ggcgcacgct tccccgcagc cttttctggg gaaccctgat
    12601 tgcctttact ctcttctttc tgtcgaatat tgtctacgtc aaggtgcttc ccctgcgggg
    12661 aatgcctgac ggtgcgggag tgcttgaacg ggggatggca ttcgccacca atgacaggct
    12721 gggaacggca gcaatttctt caattttcgg agactatgcg gccatcatca tggcggtact
    12781 gatcgtcatc tcgaccttcg ggtgcaataa cggactgatc ctttccggag ccagggtgta
    12841 ctatgctatg gcagctgata aactgttttt caggaatgcc ggaaagctta atgccagggc
    12901 tgtgcccggc aacggactgt ggattcagtt cgcctgggca tcattattgt gtctgtcagg
    12961 ctcatactcc cagcttcttg attacgtggt gtttgcggtg ctcctttttt acgttctcac
    13021 catacttgca atttttgtat tgcgccacag gagacctgag gccgacagac catacagggc
    13081 attcggatac ccggtcatta cggcacttta tatcctgatg gcagcaacaa tcatgataat
    13141 ccttcttatt tacaaaccgg ctttcacatg gccggggctg atcattgtgc tgatcggaat
    13201 cccggtgtat tatatttgga agcgatttaa caaaaccatt tagcatggag atttttcaga
    13261 ctgtatttga ctggtatatg gctaatctta actattttac catatatctc cttatggcca
    13321 ttgagagttc gtttattccc tttccctcgg agatagtggt tccgtttgct gcctggaagg
    13381 caggggaggg tactctgacg atgtgggggg tgattctctc atcgacggca ggcgccatga
    13441 ccggtgctgt catcaactac tatcttgcaa tgtggatcgg caggccggta ctgtaccgcc
    13501 tggcagacac cagatgggcg catctcctgc tgatcacaag ggagagtgtc gaaaagtcgg
    13561 agaagtactt tgtgaagcat ggcaaggcat caaccttcgt tggaaggctg gtgccggctg
    13621 tcaggcagct gatctcactg ccggcaggcc ttgcgaggat gaacatcggt cattttctgc
    13681 tttatacctt cctgggggca acgctatgga acatgattct tgcagtcatt ggatattatg
    13741 cctatgacaa gcgtgaacat atactgccat atattgactg gatcatgtac gccgtaggcg
    13801 ccatctttgt gttgtggctc tcatggaagg gataccagtt atacaggaac agaaaaaaga
    13861 cttctattca gtaagctcac cggaaagaag ccggagcccg atctctgaaa tttttgcctg
    13921 gtacagggct gtcagcctcc ggttaatggc gttaaggtag tttctctggt actccctgaa
    13981 ttccagccct gacaatgaac cgagccggta ccgctccatt gctatctcaa gtgaggcgcg
    14041 tgccacttcg gcactctggg tttcaaaatt ggtgacaatg atattgttac ggtatgtatt
    14101 gtacagcagg tcaagttctc cgagtatctc attttcaata tcaagatatg caagcctgct
    14161 gctttcggcc tcaatcttcg catttgccat cctgcggttg atctccagcc ctgagaatat
    14221 attccatgac aggttgaatc cccagttgag cccgttcgtc tggttgtatg acgatgcctg
    14281 ccaggggaac tcgttgcggg tcaggttata cccggtactg aaattgactg tcggatagcg
    14341 ttcggcgcgt actgattcaa tatcatattc tgacagtttc tccccctgac gtgcaaggat
    14401 cagctggtta ttgaaactga gagtgcgttc gcgcagcgaa tcgacatcga tctcaggagc
    14461 caatatgatt gtgtcattaa tattgaaatt gatctcatgt cctgcgttga gcaggttggt
    14521 catccggata taggcgctga tgactgtttc ctgctgggac agtacggcag agctgtcagc
    14581 gttcaggtct atccttgcct gctggaggtc gagtcccgag attacaccga gaagatactt
    14641 ttcctcggcg ttctcatacc ttgaccttga cagagccagt gatgtgaggg ccgactgcat
    14701 cctgttctgt tgaactatta tattgtagta ttcagagcag accctcatta tgaggttctc
    14761 aacattgatc ttcacccgct ggctgctcac ggccaggagt tcctgctgcc tgctgtagtc
    14821 ggtgaacatt ccgaaaccgt caaagagtct ccagttaagc gccactcctg ccgtgtatag
    14881 attggttctg gttttgtctt caccggagga ggatgacctg gcattgttag ttgtctgtga
    14941 ctgtctcccc gttccggtga gggaggggag gaaagggctg agggagacgt tgttgtctgc
    15001 aatagcctcg tcgttcctgc tgatcttgag tgaatagttg ttttcaaggg cctgcatgat
    15061 ggcctcgtcg aggctcattg tcctctgtgc tccggctgca aggggcagca ggagcagcaa
    15121 tatcattata ctaagactgg gtttggtcat tgatgctgtt ttctgcattt ttctttccgg
    15181 cgaggtaact gtaaacggag ggtaccacga agagggtaag gaatgtagcg aagaacattc
    15241 cgcccacaac ggttactccc atggtgattc ggctttccga gcccgcaccg gtggcaagcg
    15301 ccagcggcat gatgccaagt attgtggtga ggctggtcat gagaatcggg cggaaacgtg
    15361 caactgatgc ctcacgtact gcatccatca cactcagtcc tgcctgctgc ctctggtttg
    15421 caaattcaac gataagtatc ccgttcttgg ctaccatccc gatcagcata ataaggccta
    15481 tctggctgaa tatgttaagt gtctgaccga agatcaccat ggtaaagaga gcgccgatca
    15541 gagccagcgg tacggtaaca agtatgataa tcgggtcacg gaagctttcg aactgggccg
    15601 acagaaccag gtagataagc acaagtgcca gcacgaaggc aaaaagaagg ctcgatgtgc
    15661 tttcaacaaa gtccttcgag tcgcctgaca gggtggtgct gaagttttca tccaggaccc
    15721 tgtcggcaat tctgtccatc tcctcgatgc cttcgccgag agtgtacctg ccctcaaggc
    15781 ctgcagaaac ggttgctgac acgaacctgt tgtacctgta gagctgaggc gggagactgt
    15841 tctcccttat ctcgactata ttgtccagct gaataagctg ccctttgtcg tttctgacat
    15901 atgtgctggt caggtcgaac gggtcattgc gcatagcctt gtcaaactgg ctgaatatct
    15961 ggtactgctt cccgtcaagg atataatagc cgattcgctg ttcgctcatg gtcagctgaa
    16021 gtgtctgggc aatgttctga acgttgatcc ccatcattga tgccttttcc ctgttgatca
    16081 atacggtgag ctcaggcttt gtgaatttaa ggttcacatc ggaggctgaa aaataggggc
    16141 tttcagatgt catggcgagg aaatccggta taaccctttt caggtcattg aggttctttg
    16201 cctggatgac atactgcacc ggcagtccgc cacgccgtgc accgaatgtc tgctgctgtg
    16261 acacaatcgt ctgtgcgccg gtgtattgtc tcagtacagg ggaaagggca gttgcaattt
    16321 cctgctgtga acgtttgcgg tcttcctggc caaccagtct gactctcatg ttggctgagt
    16381 tgacgcttcc catgccaacc atcatcataa tattgtcact ttccggtacc tgctctttga
    16441 caaccggggc aatctgatcc atgtaagcac tcatgtactc atacgaagcc ccttctgttg
    16501 ctcgggcact gatgttgaca taaccgcggt cctcaagggg tgccatctcg gagggtagca
    16561 gggtccacat ccatgcaatc agtccaccgg ccactacaat gattatcagt gcccagtacc
    16621 tcaccttcag gaacgaggtg atggctttcc tgtagaaatt tatcatacct gagaagaatg
    16681 gctcggtggc gcggtaaaag cgcccttcca tggcattgtg cttcagcacc cgcgacgaga
    16741 tcatgggtgt aagcgagagg gccaggaacg ccgagattat cacggcaccg actatcacca
    16801 ggctgaactc cctgaagagc ctgccggtta ccccctgaag gaatactatt gggaaaaaga
    16861 cggatatcag ggcaatggtt gtcgcaatga tagcgaagta aatttccctt gaacctgtca
    16921 gagccgcatc cattggtttc atgccgtgtt ccaccttggt atagatgttt tccaccacca
    16981 caatggcatc atcaaccaca aggccgattg aaagcactat ggcgaacagc gtgaggatat
    17041 tgattgtgaa cccggccagg tacatcacga agaatgcacc gatgagtgct accggtattg
    17101 caacaacggg aataagtgtt gttctccagt tgcggaggaa gagaaagatg accagcacca
    17161 ccaggaggaa ggcaacgaag atggtattct ttacctcctt gattgactcc ctgatataaa
    17221 gagtgttgtc aaaaagcact tccatggtga tgtcttcagg aaggtctttc tccagctgtt
    17281 tgaggagtat ctgtgcgtta tcgacgatgt caatgtagtt ggccccgggc tggggaacaa
    17341 gcacaacgtt tacagacggt ttgccgttac tgagcaggtg tgaccgtatg ttttccgcat
    17401 caatctccgc ggtgccgatg tcactgaact tcactaccct gtcaccgttt tttgtgataa
    17461 tcaggtcatt gaattcgtct acggtgcgca gccttcccag ggtcctgatt gtaagctccg
    17521 tattgtcgcc ttcaattctt ccggagggca gctcaacatt ctcccgtgtg acggcattac
    17581 gtacatcaag tggggtgagc ccgtatgctg ccagcctggc agggtccatc ttcatcctga
    17641 tggcaagttt ctttgatccc catacgctca cggcactcac acctgagatg gtctgcagcc
    17701 tctccctgaa gttgagctcg gcatactggc ttacatcaat aatgtttctt gtatcactcc
    17761 tgagcgttac cgcgaagatg ggctgtgcgt cggcgtcagc cttctggaca accggaggat
    17821 caacatcctg gggaaggcgg cgcatggcgc ctgagacctt gtcacggacg tcatttaccg
    17881 ctgtctccat atctacctcg agcttgaact cgacggttat gcgtgatgat ccgtcactgc
    17941 tggagcttga tattgaacga attccctgga cactgttgat tgctgactca agaggctcgg
    18001 tgatctgcgt ttcgatgaca tccgagttgg ctccggggaa cgaggtggag acggttacaa
    18061 tggccggatc cacacttggg tagtccctga cgccaaggaa ggtgtaaccg atgccaccca
    18121 tgatgagaat cacgagcgcc ataaccgtgg ccagtaccgg tctctttatg cttaatgatg
    18181 acagactcat tcgggtcgga tgttttcgtt aaactgaatg ggtacgatta ttcggttatg
    18241 tttaccctga cagccatgtt cggtctgagc tgtatcagcc ctgtcgtcag cacggtgtca
    18301 cctgcagcca gtcctgatat gacctctatc gccgatgctg tcctggcacc ggtaaccacc
    18361 ggtatcagtt cggccctgcc attgcgtgcc acccagattt tcttctcatt catttctgga
    18421 acgattgctt cggtaggtac ctggagagca ttcatcttct cgtccgtgat cagggtcagt
    18481 gaggcgtaca ttccggggac aagtttgccg gtgcggttgt cataccttgc cctgagtcct
    18541 atcgtacggg tggtctggtc tatcctgtag tctacggcgt agacagttgc aaagaactca
    18601 tccctgtatc cttcggtctt gaatgatatt ttctttccca tcaggttctc tgatacatat
    18661 ttttcattta tggcgaattc gagcttgagg atagactggt caaacaggtg tgcgatcttg
    18721 acctgggggg taacataggt cccttcgctg acataccgga acccgatgac tccgtcgaac
    18781 ggagcgcgga cctctgtctc ggctattctg accttcagaa ggttgatgtc ggcgaggata
    18841 acgttatagt ctgtctgaag ctggtcgaac gcttcgcggc ttaccgcatc cttggagagg
    18901 agcaccctct gtcgctcaag cttctcggag agcatttttt cctgtatttc agagcggcga
    18961 agctgtgcct ggaggtcttc gtcatagatc tttacaagga ggtctccctt cttaaccttg
    19021 ctcccttcct tgaaataaac agcagtcact ttgcctgaca ccttacttac tatgtctact
    19081 tcctcgtttg ccagcagtga tccggctgac ctgataccgt tggtgaggta tgatggtttg
    19141 gctattgtgc ctgtgacagg cagttgtccc tggccacccg ggcgctgtcc ctgtcccttc
    19201 tggccaccct gcgtctgacc ctgttggcca ccttgtgtct gtccctgctg tccgcccggt
    19261 ccctgaccct gctgtccgcc cggtccctga ccggttgcct gaacctgcgc agcaggcact
    19321 tcgcctttct tcttcaggtc acggttgccc cacagaccta gccgcggtcc agcgaaccaa
    19381 atcaacagta cgagaggaat gctgatccac agagctattt tagtcccttt tttcatttat
    19441 gtgattgtat gttcaattcc tgaccccaaa ttaaaggggt ttattaaaac cattatgtta
    19501 aagtatatta aaacgaacag caggcgacta aatcatgatg ctgtttcccc tgggagatgc
    19561 ctcagtattt tatcataatc tctctgacca ccctctccat gttgtcggtt atctttctcg
    19621 acggtacggt gaatccgttg gcaaaggtgg taaaggcgat ttttcttccg ctgttggttg
    19681 tgaagtaacc tgcaaacgac tttgcgctcg ttatagtgcc ggttttggcc acaactctcc
    19741 ctttaaacat ctcatcacgg aaatagtttt tcatggttcc tgagacagcg cccatgggaa
    19801 gggatgaaat gaaggcttca gacacgctgc tgttgtacat gtggacaagc agcctgacag
    19861 tcatcagggc gctgatgttg ttattggagg ataatcctga cccgtccagg atcaccgctt
    19921 catatggttc acacccgatt gaatcgagga attgcctgat gacagcagat ccggcgttcc
    19981 aggtgccttc tccgcgtatc tcccatccca ggtgtttgcg aagggcttcc gcatacatat
    20041 taacgctctc gtggttggtg acctttacta tctcggcgag ggcgggtgag agcgtagttg
    20101 ccacggagaa acgtttttta ccttctgcgg acacacgatc ccgcactggt tgaccatcca
    20161 ccactatgcc tgctttccgc attgcggcat caagtttttt taccagggcg aggggcgggt
    20221 cggggatgga ggccctgagg gtaaagctgc tgtctgcagg tacgctgcct gtcacccagg
    20281 ccatcgggga gtaaggggca ttgtatacgt atcccctgtc agtgctgccc gaagaaagaa
    20341 gatagctgtc aaggataatg tctttcccgt aatcctgaac tgaatcgatg acagccgcct
    20401 cgccctcagc tctgcctgaa atgtatattt tgtattcgtt gttgtttatg ttgatatcat
    20461 agaccccggc tccgtagtac tgtcccaggt cgccccatgc ccatcccgcg ggtgcaggat
    20521 tgaagtcata aatggattct cctgctgcca cgcggcccct gacctttttc accccggcag
    20581 ctttcaatgc actgacccaa gcggcggtca cgtcgccata gtattccgag aaatactctg
    20641 agccaagtgc agggtcacca cccccgatga tcaccacatc accctcgagg atcccctttt
    20701 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttttgttca agtgttgaaa
    20821 gcaggccgca ggctgcattg atatcctgac cgcggcttgc gcggatggtt gttatgatgc
    20881 cctttgcatt caggctgtca cggaaagcct ccatcccgcg ctggtcagga ctgctgaatt
    20941 ccgatccggg gattgaatgg aagcgtatca ggttaatccg gcagcggatg ccgttgagta
    21001 tcctggccag ctccctgaca tgtgcaggag tatcattcag ccccctgaaa acaatgtatt
    21061 caaacgagac tcttctctgt ctgccgaaat cgaagcccct gatggtgtcg aggacctcct
    21121 tcaccgggtg ggctttctga gccggcatca gtgactgccg ttcactgtca aacggtgagt
    21181 gcaggctgac agccaggtgg catttgctct taccgaggaa ctcctctatc cttccggtga
    21241 tgcctatcgt gctgacggtg attcttgtcg ggctccatcc gtaaccccag ccggaggtca
    21301 gaatttcgag gctgcggagt acctcctcca ggttgtcaag gggttctccc atccccatga
    21361 atacgatatt ggtcagtgag tcatactccg gcaggctgcg gaactggttc agtatctcat
    21421 tggagctgag gttcccctga aaaccctgac gtgctgtcat gcagaaacgg catcccatgc
    21481 ggcagcccga ctggacggag aggcagatgg ttgcccggtc gcggtcaggg ataaaagcag
    21541 tttcgatgaa ccggttttcg cttacacgaa agagatattt ctttgtgcca tcactggcgg
    21601 atgattctga tacaggcgga ttaagccccg tttcataatc cttttccagg ataccgcgcg
    21661 ctgcaaggga gagatcagtc atcgcgctga tgtgaggagc ttccctgcga tagagccatc
    21721 ccgctatctg cttcgccgca taaggtggca gtgaggcatc tttgacaacc tctgacagct
    21781 catttagagt caggccgaaa agcttcttct tttccatcag aaacgatagc ctgccaggta
    21841 aaggtcaagg atgttcctga atggctttgg cggtttcagc tccgcattca ggagagtctt
    21901 cagaataagc cttctcatat catatgtttc actggatttc cttttctccg ctgaacacga
    21961 aggtggccgg gccggtcagg taaatgccgg tagctttttc tccggtaaga tcgaacctga
    22021 ccgacagcat tccgcctctg gcctccacct ctgcagcagg ggtgacaatt tttccggtat
    22081 atgctgccgc aatggcagat gccgttattc cggttccgca agccagtgtt tcgtcttcca
    22141 ctccccgttc gtaggtgcgt acccgaatcc ttccgttgtt aatgcttgcg agattgacgt
    22201 tcaccccttc aggtgcatag gcaggtgacc agcggagggc cctgccgaag gagaccatgt
    22261 cagt
//
LOCUS       DAOPXU010000041        22249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_2374_length_22249_cov_13.577228, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000041 DAOPXU010000000
VERSION     DAOPXU010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22249
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2374_length_22249_cov_13.577228"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2713)
                     /locus_tag="PKK03_05830"
     CDS             complement(<1..>2713)
                     /locus_tag="PKK03_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX83953.1"
                     /translation="AMSETDGSYAITVPSAESILEFSYVGYLKQQIRVGTRTVINVQL
                     VMQYNSISDVVVVGYATEQKALLTGSIDVVSSDAIRDIPVMNIDNLMQGQASGVQVTQ
                     NSGTPGGATSVRIRGVNSISGSNQPLYVIDGIPVVTGDYGQVGYEGQGINAISDLNPN
                     DIESISILKDAAAASIYGARASNGVIVITTKRGANQKTQVSLNSYYGVQQVWRTLDML
                     DARGWMEYRNDLAGTPVFSQEQMENITIDTDWQDIIFRTAPNANYELTANGGNENTKF
                     FLSGDYFTQDGILIGTDYQRLNGRVNIDHNLTEKFTVGASVGLTYAKTNRVEGDQTLH
                     GPLPNGISTPAIYPVYNEDGSYNQSGPYSNAVSIANEAINQNFSFRTLANSYLTWHIL
                     PRLNLTTKWGADFLNFREHAFEFNTVQGQKYNGLGFETYTNVLNIVSNNVLTYDFAVG
                     ENKFGFLAGYSFEKYQDRSSFIRAQDYADESLEYINSASTIASASSDANNSGLNSFFG
                     KVNYDYKTKYIISLSGRFDSSSKFGENNRTGFFPAASVAWRMGEESFMDNLPAISELK
                     IRASYGLTGNDDIPHFLYAELYGVTSYNSASAIYPSSIPNPDLKWETTAHLDIGIDLG
                     FLENRIFLTADYYNKQTKDLLLARPLPGSSGFSSITENIGQVENKGVELNLTTNNLVG
                     RFKWNTVANLSVNRNKVLKLYNGQPIDDIGRGGNRVMEGQPIGIFYSYEWLGIDPSTG
                     DCVYSDLDKNEQITTHDRTIVGNPHPDFTGGITNNFSYQGFDLSVFLQFSYGNDIFNG
                     SRLYLESLQGGDNQVADIVRRWQQPGDITDIPRATSDAVAAASNKRVSSRFIEDGSYL
                     RIKNLTFGYTFSDNLMSRLNLSSLRLYLTCQNLFTFTNYSGLDPEVNY"
     assembly_gap    2714..2813
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3003..4316
                     /locus_tag="PKK03_05835"
     CDS             3003..4316
                     /locus_tag="PKK03_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX83954.1"
                     /translation="MKILLLTVWLSLGGVICAGAQSLGRVGSATDISPSVSAGTVLMG
                     GGADVDAAFTWMINKSGGGDFVVIRATGTNAYNSYIYGLGSANSVETFLINSRELAND
                     SAVVQAVRKAEALFFAGGDQNDYIRFYRGTALGSALNYLANVKHIPVGGTSAGMAIQG
                     SVYYDGITNVVSEEVLLNPYRSGTGIHYNDFLQNPFLKKTICDTHFNTKGGTTDNGIR
                     GRQGRLMSFLSRMITDSAMTDVKGIACDEKTAVCIDQDGIAMVAGVGKAYFLRQWCAA
                     PETCVQGQPLTWTNGARVYVIPGPGSYTAVPAAAKSVDLKDWTTVSGGTYEYWTVTSG
                     ILTLGQTTGTPSVCNLTGVASPAEDDDICIYPNPAGDRITISSDVPFNNSTITLYNSL
                     GQIVQRKSNLNGNQVNFNSSALSCGTYILEIEQTDNMLRRKVIKL"
     gene            complement(4390..5046)
                     /locus_tag="PKK03_05840"
     CDS             complement(4390..5046)
                     /locus_tag="PKK03_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83955.1"
                     /translation="MKPAICILLLASMMWSCKNNAGYIEYFVRYTTEDISLKSGLADP
                     ELYHTGMGDYITSITPSYFEQEFEVPAGYGTMQIIGQNGTYTTEPGTGKRYIKICHQP
                     LIEPYFGVPDRQPWGYFFGNTESTYVFNRECAELPASEDSPDGGRDCMIRSHQFTPLA
                     VTMPEEDETIGMYSTVSFNTDNLIQVYAGMDNTPYTQDDVFTYAPNYWERLKVRLEVR
                     "
     gene            5182..5979
                     /locus_tag="PKK03_05845"
     CDS             5182..5979
                     /locus_tag="PKK03_05845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX83956.1"
                     /translation="MKTAVITGGTRGIGSALVRSFLESGWNVAYSGTSETNVEKSLKL
                     LDGQFERGRYAAFKCDVSVEADLVSLWDRAVKTFGRVDIWINNAGTSSDQTPFHELPT
                     EIFTRIIDTNVRGLMLATHVVYNRMLKQGHGAIYNMEGLGSDGRRITGLTPYGTSKRA
                     VRYFTYAFAEEVKEGPVIVGTISPGMVLTDLTMEQIRKDPGNSKKLIKIYNILANEAD
                     TVAPFLVRKMIGNRKHGAKINWLTNWKVVRRFLIAPFSRRDIVSRYL"
     gene            complement(6062..6973)
                     /locus_tag="PKK03_05850"
     CDS             complement(6062..6973)
                     /locus_tag="PKK03_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83957.1"
                     /translation="MKSKLIAVWLFAAIVATATGQQVPDTGYSPVIKNPAYEQGLGPL
                     VYIDEGHNNFHTRGDRYLPFARLVERDGYVTEGYAGQFEPERLKKCRILVISNALHET
                     NATRWHKPVYSAFTPGETETVRRWVEEGGSLFLIADHMPMGGAAAEMAAAFGFGFTDG
                     FALDTAQAGPALFCRADGTLAENILVNGRSADERVDSIFSFTGQAFTVPDDARTVLTF
                     NEKYLLLLSDTAWVFNEQTVYKPIKGWSQLAFREYNRGRVVMSGEAAMFTAQLAGPQQ
                     FPAGMNAPYARNNYRLLLNIIHWLDGR"
     gene            7191..7778
                     /locus_tag="PKK03_05855"
     CDS             7191..7778
                     /locus_tag="PKK03_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX83958.1"
                     /translation="MKTKVLLTAAILALLPVALTAQGEEKRAGFEVNGGLSLSTCAPG
                     QAITNAGAGAEALLHYRLTKHLGIYGGWGYNAFRNDYEPAGHRCDFEETGYILGLQYK
                     RKIEFSNASVFVRGGYQYKHIEIEDPTGELLFDTTHGPGWQAGCGIDLPLTEKWSLLS
                     ELKYNALLEREGHVPDAPLPDRNYFSVRLGILMNF"
     gene            7803..8177
                     /locus_tag="PKK03_05860"
     CDS             7803..8177
                     /locus_tag="PKK03_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83959.1"
                     /translation="MNIRETILIMTTVAMTALSACEPIASDRFGFDLPVGPRSGGINN
                     VEVDFTVSAIRLTGNIIASEGALVAEIMSPSGAVVFSASVEAPGEVHIDKSLPVEEGE
                     WRMRYFSLNGKGVIRMHLNLVR"
     gene            8268..9362
                     /locus_tag="PKK03_05865"
     CDS             8268..9362
                     /locus_tag="PKK03_05865"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNX83960.1"
                     /translation="MTQSFMKRLNGLIPLPKLVRFVIGISVAAQMIVIIYNNFSGFYP
                     LQNFTHFLLRLTRGVLLTVPAAMLVAVPDLLIIGRLNALFPWHRRVFPRVVIQFMAAV
                     TLAATVSVLMTLTANSLTPYSEPLNLVLLYNALIYAVVNIIIMGVLEAWVFYIRSAEA
                     ARKAEALERELSQMRFEMLKSQINPHFMFNSLNVLSGLIEKDVSRAQEFIDEFSQIYR
                     YVLETVEQHTTTLGKELEFMRSYLFLQQMRYGEDLTWSVSVPGDLLGAVLPPLSLQVV
                     LENAIKHNIVNHSKPLHIGITASDMMLTVANRLQPKISAPASTGLGLKNLTRRYSLIT
                     GRLPQFAVENDHYIARLPLLNPEGNDSADN"
     gene            9343..10098
                     /locus_tag="PKK03_05870"
     CDS             9343..10098
                     /locus_tag="PKK03_05870"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNX83961.1"
                     /translation="MIALIIEDESLAAEKLEKMLAEIDPSVTVTAKLGSIKDAVKWLM
                     QNRADIIFLDIQLSDGISFSIFEQVAVNTPVIFTTAYDQYAVRAFQLNSIAYLLKPIR
                     RSDLAESLNKYQSLRAAFSIDFDALMAQLQGRDPGYRKRFLVQTGEKLRKIEVTEIAW
                     FYVLEKSAWLKTFQGNSYPAELSLEALEAVLDPSVFFRINRKYLVSMNSIAGMTAWSR
                     GRIKLELKPKADDELDTIVSIDRSSAFRKWLNA"
     gene            complement(10088..10897)
                     /locus_tag="PKK03_05875"
     CDS             complement(10088..10897)
                     /locus_tag="PKK03_05875"
                     /inference="COORDINATES: protein motif:HMM:NF039830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5692 family protein"
                     /protein_id="HNX83962.1"
                     /translation="MNIPVWQYLVSMPLYIGILLILVELMRKYYKASALFWILALFTF
                     PLWAAQLDGWFRWVKTFSVLVPTALILGLGRLAHADDRPGWLSFFRKDWVLWALYAVL
                     GLNILEASLKDLSLGNYFNAISGFILIVTMPLFKSKGMRNGWAFSKGKPWDMLAYTDP
                     MWNFLYTTWNIAFVYAENPGYAASSLCILLAAEIYPIIKRRPELYVQARAYTLAVHIL
                     IRATYDIFTPIMDSSAFASERAVWYWGLINVVMHVPYLFWYFIKRRKSFRR"
     gene            complement(10945..11520)
                     /locus_tag="PKK03_05880"
     CDS             complement(10945..11520)
                     /locus_tag="PKK03_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX83963.1"
                     /translation="MRSKDYGKVELVYEAGLELISREGIAGLTMKGLAARSGVATGTL
                     YIYFRSKEELFHRMYLYYRNRSLDRFMQGYDPVEPFRIGLKKVFVNYLTHRIEHHSES
                     IFLEQYYRSPYITEEQNALAEAMKHPVYEVIKRGKREMLIKNDIDDEMLFLAMIGFVR
                     ELADEHVTKQYKMTPERIEKAFQLAWDMIKM"
     gene            11760..13538
                     /locus_tag="PKK03_05885"
     CDS             11760..13538
                     /locus_tag="PKK03_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNX83964.1"
                     /translation="MEHIVSRTFDLLDRYRELYNKADALCFKHNGNWQKYSTAEYIEH
                     AYSFCYGLCESGFRRGDKIITVSANRPEWNFTDMGMSMLGVVHVPVFTSLSATEYEYI
                     IRNSGARMIIISDKKLCRTLEPALTATGHSCPAFTFDKIDGAANWLDIVEKGRNCSAE
                     TKQQVEEIKKLIKPDDFATLIYTSGTTGKPKGVMLSHRNMVSNFISAASVFNLKPTDK
                     YLSILPLCHVGGRLGNYQTQFSGTSIYYAESMGTISINMKEIKPDGFDAVPRVLEKFY
                     DVIISKGKSLTGIKKSLFFRAVNLGLNYQPFGENGWLYERKLRIADKLIFSKWREALG
                     GNVRIVGCGGASLQPRLERVFWAAGIKIINMYGLTETSPIITINRTEKGKVKLGSVGM
                     TIEGVEVKITDDGEILCKGPNVMLGYYNDPELTASVFDEEGWLHTGDIGHIEEGKFLM
                     VTDRKKEIFKLSNGKFIAPQRVENIFKESPAIDQIMVIGEHEKFASALISPNFKYFED
                     WKTSRKVNYSSNDELITLPEVLQFFSSEVNKMNKRLSPPERINRFRLVKDEWSPATGE
                     LSPTLKLRRKFIHEKYSEVVEQVYNK"
     gene            13565..14734
                     /locus_tag="PKK03_05890"
     CDS             13565..14734
                     /locus_tag="PKK03_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83965.1"
                     /translation="MKLIEVTDRKTERMFLDTARVIYRNDDTWVCPLDRHINGVFDPA
                     VNPYFKHGTATRWVLTGDDNSLIGRIAAFIDFNIAKTYDQPTGGTGFFECIDDDAAAG
                     MLFDTAREWLRERGMEAMDGPINFGETDKYWGLLVEGFTHPSFEISYNPPYYQGLFER
                     YGFQTWFRQEGFHYDLTKPLDTRFRKIAQWVAEKPGYEFRHFTWKEQDRYVADFAEVF
                     NEAWASFKANFEPLEPDYIKGTLKKAKAILDEEFIWLAYFEGKPIAIYLMFPDLNQIL
                     RHLNGKLNLVSMIKFLWLKKRNTMTRARGMLMGVIPKFQKLGIESAFILKVREAIEHK
                     PHYKEVEFSWVGDFNPKMRKIFISVGSVSVKHYITYRYLFDRTKEFKRYPIPEIK"
     gene            14731..16176
                     /locus_tag="PKK03_05895"
     CDS             14731..16176
                     /locus_tag="PKK03_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNX83966.1"
                     /translation="MKYDVIVIGAGLGGLTAGAKLAREGMKVLVIEQHSQPGGCATSF
                     RRGDFTLEVGLHEMDGPGSRDMKNRIFSELDVTGNVEFIHVPEFYRFIRGDLAVTIPH
                     DPQLAAARLTEMFPEEKEGIAKYFDQLLSPKKKPAGETEPDKSVGSFLDSIISNDDLK
                     LILLGNLGYFHDDPYSLSLIYYSVAEGSYYTNGASFIKGGSQRLSNHLAGYIRSHGGE
                     VLLNHVVTGVTVEAGRATGVTFRKRGGKSQNPEEATADEIVANCSIPALADMLPPEQG
                     ALLREEYSGLNTGASLLTVYFGFDRPLKELGHSYYSAFIYDRSVRSQADIAANNRDDF
                     SRRSFTFVDYGQVDSGLAPEGKSVGAICCIDYLADWEDLGSEEYKLKKEYVAQTFIDR
                     LGEVIPGIRDIIGYYEVGTPVTVKRYTLNPGGAVYGFAQNPGRKVLDSFAPVENLHFA
                     SAWGRTGGGFSGAICGGYLSAFNLLRKRARH"
     gene            complement(16182..17303)
                     /locus_tag="PKK03_05900"
     CDS             complement(16182..17303)
                     /locus_tag="PKK03_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX83967.1"
                     /translation="MNRLKAKTHHMLILVITALLIIVAMPLLMLVLVSPGKTRSCPAA
                     GSGSSICEKRFEEIGGVRQGMFIKGADTANPVLLFVHGGPSFSEYFLAEKYPTGLEDH
                     FTVCYWDQRGGGLSATREVTMESLNLGQLAADMIEVTNYLRSRFGKERIYILAHSGGT
                     AFALRAVAENPELYHAYIGMAQITRQAESEKLAYRYMVDSYSSSGNRKMAAKFREYPI
                     IEDEAYLLPFFNSVLRDKSMHELGIGTMRNMNSIMTGVFYPVWLCRAYTIREKFNIWR
                     SKFSFVNKSGLRAEVLKLDMSVAVPELGIPAYFISGRHDLTVSHDLSEEYCNKLESPL
                     KRFYTFKNSAHSPMFEEPERFLEIMITDVLNKAITLTND"
     gene            17458..18336
                     /locus_tag="PKK03_05905"
     CDS             17458..18336
                     /locus_tag="PKK03_05905"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNX83968.1"
                     /translation="MTPHAEKLVITVSDTVNAPLQRVWELFTDPLHIIHWNHASDDWQ
                     TSYAENDLRPGGAFLSRMEARDGSEGFDFTGTYSEIVPGKSIASTLDDGRNVHTSFSE
                     KAGVTLVTESFEAEDQNSAELQRAGWQAILDSFKRYVEGYGPIEKLHFEILINNSPEN
                     IFRIMTGSDTYNKWTTEFNPTSRFYGSWNKGEKITFLGTDADGKTGGMVCRIRENIPG
                     KFLSIESLREFVDGSEDAGTGKENSWSGALENYTLKPAGSKTLLCIDTDVPAEHRPFL
                     LEAWPKALETLKELCE"
     gene            18527..19204
                     /locus_tag="PKK03_05910"
     CDS             18527..19204
                     /locus_tag="PKK03_05910"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HNX83969.1"
                     /translation="MNALRKTSLIFGLFFAGTFVFSIPALFFYDPLLHDSGYIHGGGF
                     DRRASMGALFEILTAICNIATAIVIFPVVKRVSESAAVGYITSRTVESVMILSGVVSL
                     MAIVTLRAGFEPGGDTVALTMTGQGLLAFHGWTFLLGPQFGSGFGNGILLGYLMYRSG
                     LVPRRMALLGLIGGPLAFAGGVLVLFDLLKPMSAGLFALTGMEIAWELSITFYSLFVG
                     FRPTQEK"
     gene            19211..20119
                     /locus_tag="PKK03_05915"
     CDS             19211..20119
                     /locus_tag="PKK03_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNX83970.1"
                     /translation="MEKMKAVVYEKYGPPDVLKLTQVEKPVPGDEEVLIKINATTVTA
                     ADWRLRKADPFLARIFNGLFKPGKIKILGFELAGEIEETGKDVRLFKKGDQVFAATGF
                     TFGAYAEYKCIPEGGTIALKPVNMTFEEAAAIPVGGLTALHFLRDKGHIRGRSKVLIY
                     GASGSVGTYAVQLAKYYGAEVTAVCSTPNVELVKSLKADKVIDYTKNDVSREGELYDL
                     IFDAVGKISLSACRKIMKPGGKYISVRRGTAKSREGDLAFLKKLVESGTVKPVIDRLY
                     DLAQIAEAHAYVEQQHKKGNVVVTVK"
     gene            complement(20197..20988)
                     /locus_tag="PKK03_05920"
     CDS             complement(20197..20988)
                     /locus_tag="PKK03_05920"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNX83971.1"
                     /translation="MRYIICLLALLIPSLVQAQWKELSPGLEYRAQSVTLPEYSYPIE
                     INAIRIDLQKWDLVFRGISQEADGPLKTPKEWCEKYSLTAAINAGMYNDDYQTHTGYV
                     RDGEHRSSLHRNNYKSLLAFNPAKGKQVPPARIFDLDQPGVTIDSVLKDYSTVIQNLR
                     LIKRLGISVWPDQGNRWSEAAIAEDKQGRILFLYCPSPVSMHDFNKILLYSDMEIVAA
                     QHLEGNAPAQVYLKAGSTELILSEDPDLMLPLPNIIGIRPKPVRK"
     gene            complement(21471..>22249)
                     /locus_tag="PKK03_05925"
     CDS             complement(21471..>22249)
                     /locus_tag="PKK03_05925"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNX83972.1"
                     /translation="LFLFNQDAALMDYYERGLYNHILASVAEHNAGNTYHVPLRPGSM
                     KQFGNEDMDGFSCCNGTALESSTKLQNSIYFRSADNRALYVNLYVPSTLTWTERKITV
                     TQATAYPKEDRTQLVIGGKGRFDLNVRVPHWATKGFLVKINGRDFSVDAVPGSYMTIS
                     RKWKKGDTVELRMPFKFYLEPVMDQQNIASLFYGPVLLAAQEPAPRTEWRKVTLDASD
                     ISKSITGDPSKLEFMIDGVVYKPFFDTYGRHSVYLDVELK"
BASE COUNT         5651 a         5632 c         5540 g         5326 t
ORIGIN      
        1 ggtaattcac ctcaggatca agtcctgaat agttggtgaa tgtgaacagg ttctggcagg
       61 tgagatagag cctgaggctc gaaagattca gtctgctcat aaggttgtca ctgaaagtat
      121 aaccgaaagt caggtttttg atcctgaggt acgatccgtc ctcaatgaac ctcgaggaga
      181 cgcgtttgtt cgacgctgca gccaccgcat cggaggttgc ccttgggata tctgttatat
      241 caccgggctg ctgccatctg cgtacgatat cagccacctg gttgtctccg ccctgaagcg
      301 attcaagata caggcgcgat ccgttgaaaa tgtcattccc gtatgagaac tgaaggaaaa
      361 cactcaggtc gaaaccctgg taactgaagt tgttggttat gccaccagtg aaatcaggat
      421 gagggtttcc gacaatggtg cggtcatgcg tcgttatctg ttcgttcttg tcaagatcag
      481 aatatacgca gtcgcccgtc gagggatcaa tgcccagcca ttcataactg tagaaaatgc
      541 ctatgggctg accctccata acacggttgc ctccgcggcc aatgtcatct atcggctgtc
      601 cgttgtacag tttaagcacc ttgttcctgt tgaccgacag attggctacc gtgttccatt
      661 tgaaccggcc caccaggttg ttggtggtta aatttagctc cacacccttg ttttcgacct
      721 gtccgatatt ttccgtgatc gaagaaaatc ctgaacttcc cggtaacggc cttgcaagaa
      781 gaagatcctt cgtctgcttg ttataataat cagcggtaag gaaaatacgg ttttccagaa
      841 atcccaggtc gatgccgata tccaggtgcg ccgtggtttc ccatttcagg tccgggttgg
      901 ggatgctgct cgggtatatg gctgaggcgc tgttgtagga ggtgacaccg tacagctcgg
      961 cataaaggaa gtgggggatg tcatcattcc ctgtgaggcc gtaactggcc ctaatcttta
     1021 actcggatat tgccgggagg ttgtccataa agctttcctc gcccatcctc caggcaactg
     1081 aagctgccgg gaaaaatccc gtccggttgt tctcaccgaa tttcgaggat gagtcaaacc
     1141 ggcccgaaag tgagatgata tattttgttt tatagtcata gttgaccttt ccgaagaatg
     1201 agttcagccc cgagttgttc gcatcggaac ttgccgaagc gatggttgag gctgagttga
     1261 tgtattcaag gctctcatct gcatagtcct gtgccctgat aaacgaagac ctgtcctggt
     1321 atttttcaaa actgtaaccg gccaggaacc cgaatttgtt ctccccgaca gcgaagtcat
     1381 aggtgagcac gttgttggag actatgttca gcacattggt gtatgtttca aatcccaggc
     1441 cgttgtattt ctgcccctgg acagtattga actcgaaggc atgctcgcgg aagttcagga
     1501 agtcagcacc ccacttggtg gtgaggttca ggcggggcag gatatgccat gtcaggtaag
     1561 agttggccag tgtcctgaat gagaagttct ggttgattgc ctcattggca atggaaacag
     1621 catttgaata gggcccgctc tggttatagg agccgtcttc attatatacc gggtaaatgg
     1681 caggagttga aataccattg ggaagaggcc cgtgcagtgt ctggtctcct tccacacggt
     1741 tggttttcgc ataggtcagt ccgacgctgg caccgacagt gaatttctca gtaaggttat
     1801 ggtcaatatt cactcttccg ttcagcctct gataatcagt gcctatcagt atgccatcct
     1861 gggtgaagta gtcgccgctc agaaagaact ttgtattctc gttcccgcca tttgccgtca
     1921 gctcataatt tgcattcggt gcggtgcgga aaataatatc ctgccagtcg gtgtcaatgg
     1981 ttatattctc catctgctcc tgcgaaaaga caggcgtgcc tgcaaggtca ttcctgtatt
     2041 ccatccaccc ccgtgcatcg agcatatcaa gtgttctcca cacctgttgc accccgtaat
     2101 atgaattcag gctgacctgt gttttctgat tggcgcctct ttttgtggtg atcacaataa
     2161 ccccgtttga tgcacgggcc ccgtaaatcg aggcggcagc cgcatccttc aggatactga
     2221 ttgattcgat atcattgggg ttaaggtcgg atattgcatt gataccctgc ccttcatatc
     2281 ccacctgtcc gtagtcaccg gtgacaacgg gaattccgtc gataacatag agcggctggt
     2341 tggatccgct gatggagtta acacccctga tcctgacgga agttgcccct ccgggtgttc
     2401 ccgagttctg ggtaacctga accccgctgg cctgcccctg catcaggttg tcgatattca
     2461 tgacagggat atccctgatc gcgtcagaac tgaccacatc tattgatccg gtaagaagag
     2521 ccttctgctc cgtggcataa cccaccacaa ccacatctga aatgctgttg tactgcatca
     2581 caagctgcac attgatgaca gttcttgttc ctaccctgat ctgttgcttt aggtaaccca
     2641 cataagagaa ctcgaggata ctttctgcgg aaggtaccgt aattgcatag gatccgtcgg
     2701 tttcggacat ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaggttgt
     2821 cagttcctta tgttctgcag gcattggctc aaaaatgagg ttcaccctat aaaattaggt
     2881 ctttttgata ttactttttg atcactatgg aaattttata tttctttaat aaatcctgat
     2941 ctcaggatcc ccgttaccgg gggattttgt taattgcagt tacccaaaag agcagattgt
     3001 gtatgaaaat attactcctg accgtctggc tttcccttgg tggtgtcatc tgtgccggag
     3061 cacaaagcct ggggagagtc ggctctgcaa cggatataag cccatcggtt tcggccggaa
     3121 cggtgctgat gggcggaggc gctgacgttg atgctgcttt cacatggatg atcaacaaat
     3181 cgggcggggg agattttgtg gttattcgtg ccaccggcac aaatgcttac aacagttata
     3241 tatatggtct gggttcagcc aattctgttg agacctttct gattaattcg agagagctgg
     3301 caaatgattc tgccgttgtt caggctgtaa gaaaggctga agccctgttt tttgccgggg
     3361 gtgatcagaa tgattatatc aggttctacc ggggaaccgc gctgggctcc gctttgaatt
     3421 acctggcaaa tgtcaaacat atccctgtcg gaggcaccag cgccggcatg gcaatccagg
     3481 gctccgttta ttatgacggc atcacaaacg ttgtgtcaga agaggtcctg cttaatccgt
     3541 acaggtcagg caccggaata cactataacg atttcctgca gaatccgttt ctgaagaaaa
     3601 caatctgcga cactcatttt aatacaaagg gtggcaccac agacaacggc ataaggggga
     3661 gacagggaag gctgatgtca tttctgtcac gaatgattac agactcggca atgacagacg
     3721 tgaaaggaat tgcctgcgat gaaaagaccg ctgtctgcat tgaccaggat ggaattgcaa
     3781 tggtggcggg tgtgggcaag gcatatttct taagacaatg gtgtgccgct ccggaaacat
     3841 gcgttcaggg acagccactg acatggacaa acggtgccag ggtttatgtg ataccaggac
     3901 cgggaagtta taccgccgtc cctgcagctg caaaatcggt ggatctaaag gattggacaa
     3961 ctgtctcggg cggaacctat gaatactgga ctgtcacttc cggtatactt accctcggac
     4021 aaacaaccgg aacgccatca gtgtgcaatc tgacaggcgt ggcaagcccg gctgaagacg
     4081 acgacatatg tatctatccg aatccggccg gggacaggat tacaatttca tcagacgtac
     4141 cttttaataa ttcaaccata actctatata acagcctggg acagatagtg caaaggaaaa
     4201 gtaacctgaa tggcaaccag gtaaatttta attcatctgc tttatcatgc ggaacttata
     4261 tcctggagat tgaacagaca gacaatatgc tcagacggaa agtcattaag ctctgaacat
     4321 agaaacaggg ttgccgtagt cctgtagtat gtaaccggta ttcagcgcgg cttgatgtcc
     4381 gaagtagcct cagcgcacct ccagccttac cttcagtcgt tcccagtagt tgggggcgta
     4441 ggtaaatacg tcgtcctggg tgtagggagt attatccatc ccggcataca cctggatcag
     4501 gttgtcagtg ttgaaactga ctgttgaata catcccgata gtctcatctt cttcaggcat
     4561 tgtgacagca agcggggtaa actggtggct tctgatcatg caatccctgc ctccgtctgg
     4621 ggaatcctct gaggccggca gctctgcaca ttccctgttg aaaacatatg tcgactctgt
     4681 attcccgaaa aaatatcccc atggctgacg atctggaacc ccgaagtatg gctcaattaa
     4741 aggttgatgg cagatcttaa tataacgctt acctgtcccg ggttcagtag tataggtgcc
     4801 attctgcccg attatctgca ttgtaccgta accggcaggg acttcaaatt cctgctcgaa
     4861 atagctgggt gtgatgctgg tgatatagtc acccatgcct gtatggtaga gctccggatc
     4921 tgccaaccct gatttcagac tgatgtcttc agtcgtgtat ctgacaaaat attcaatata
     4981 gcctgcgttg tttttgcagc tccacatcat ggaggccaga aggagtatac agattgctgg
     5041 tttcatgtga cttattaatt gatgaacttg tacaataaaa acactccagg agccaggaca
     5101 ttttaatctt gttctggcag gcctgaatgc cggaaatctg aaagacaaat aattgtagat
     5161 ttacttcact ataccataag catgaaaaca gcggttataa caggcggcac caggggcatt
     5221 ggctccgcac tcgtaagatc ctttcttgag tcgggatgga atgttgccta ttcagggaca
     5281 tcggaaacaa atgtggaaaa atctctgaag ttactcgatg gtcagtttga aaggggaaga
     5341 tatgcagcct tcaaatgcga tgtttcagtt gaagctgacc tggtcagcct ttgggaccgc
     5401 gcggttaaaa ccttcggccg ggtggatatc tggatcaaca acgccggcac ctccagcgac
     5461 cagactccct ttcatgaact cccgactgag atcttcacgc ggattattga tacaaatgtc
     5521 aggggactca tgcttgccac acacgttgtc tataacagga tgctgaagca gggtcacgga
     5581 gccatctaca atatggaagg cctcggcagc gacggccgcc gtatcacagg cctcactccg
     5641 tacggaacct ccaaaagggc ggtcagatat ttcacttatg cctttgctga agaagtaaag
     5701 gaaggtccgg tcattgtggg aacaatcagt cccggcatgg tcctcaccga cctcacaatg
     5761 gagcagataa ggaaggatcc gggaaacagc aaaaagctta taaaaatcta caacatactg
     5821 gccaacgagg ctgatactgt tgctccgttt cttgtccgta agatgattgg aaacaggaag
     5881 cacggcgcaa agatcaactg gctgacaaac tggaaagtag tccggagatt cctcatcgcc
     5941 cccttctccc gccgtgacat agtaagcagg tatctctagg caatctggtt aaaaatatcc
     6001 gggaattcac agcggaacct gacctgcctg ttgaactcaa tgatcatgga ggcaaacacc
     6061 ttcatctccc gtcaagccag tgaatgatat tcagcagcaa ccggtagttg ttgcgggcgt
     6121 aaggggcatt catgccggcc ggaaactgtt gtggccctgc cagctgcgcg gtgaacatgg
     6181 cagcctcacc cgacatcaca accctccctc tgttatactc cctgaaagca agctgcgacc
     6241 acccttttat cggcttgtaa accgtctgtt cattgaaaac ccaggctgtg tcagaaagga
     6301 gcagcaggta cttttcattg aaagtcagca ccgtacgggc atcatccgga acagtgaatg
     6361 cctggccggt gaatgaaaaa atgctgtcga ccctctcgtc ggcgctccgg ccgttaacca
     6421 ggatattttc cgccagggtg ccgtcggccc tgcagaagag cgccggccct gcctgagccg
     6481 tgtcaagggc gaagccgtca gtgaacccga acccaaacgc tgcagccatc tcagccgcgg
     6541 cgcctcccat gggcatatgg tcagctatca gaaaaagtga tccgccctcc tcaacccacc
     6601 tccttactgt ctcagtctcc ccgggggtga atgcggaata aaccggtttg tgccagcggg
     6661 tggcattcgt ctcgtgcagg gcgttggaga tgaccagtat ccggcacttt ttcagcctct
     6721 ccggttcaaa ctgcccggca taaccctcag taacgtaacc gtcacgttca accagcctcg
     6781 caaacggaag atagcggtcg ccacgtgtgt gaaagttatt atgcccctcg tcaatatata
     6841 caagcggacc cagtccctgt tcgtaggcag gattcttaat caccggggaa taccccgtgt
     6901 ccggcacctg ctgccctgtg gccgtggcaa caatcgcggc aaacagccag acggcaataa
     6961 gtttactctt catggcatag cgtttaattc tcacccgaag ttatgcatag tcagccatat
     7021 ttcatgctgc atccggtttt attcaccaga aactgccatc actttcagcc cccggatatg
     7081 caccttcagc accctctttc gcacccccgg caccttttat tttcggagcc tccgggcgcc
     7141 ccctactttt gcttcgacaa acaaacacta atccttttaa accaattaag atgaaaacaa
     7201 aagtattatt gacagcagca atcctggcac ttctgcctgt ggcactgact gcccagggcg
     7261 aagagaaaag agccggattc gaagtcaacg ggggactctc cctctcaacc tgcgcaccgg
     7321 gtcaggccat cacaaatgcc ggcgccggcg ccgaagcact gcttcactac cgactgacta
     7381 aacacctcgg catctacggt ggatggggct ataacgcctt ccgcaacgac tatgaacctg
     7441 ccgggcacag atgcgatttt gaggagacag gctatattct gggtctgcag tacaagcgga
     7501 aaatcgaatt ctcaaacgcc tcggtctttg tcaggggcgg gtaccagtac aagcacattg
     7561 agatagagga cccgacaggc gaactgctct tcgatacgac ccacggaccc ggatggcagg
     7621 caggatgcgg catcgatctc ccgctgacgg aaaaatggtc actcctctcc gaattgaaat
     7681 acaacgctct gctggaaagg gaaggacatg tccctgatgc tccgctcccg gacagaaact
     7741 acttctcagt cagattgggt atcctcatga atttctgatc agtgcaataa aactgaatga
     7801 aaatgaatat cagggaaacg atcttgatca tgacgactgt tgcaatgaca gccctgagcg
     7861 cctgcgaacc catcgcgtcc gaccggtttg gcttcgacct gccggtcggc ccccgctccg
     7921 gtggcatcaa caacgtggaa gtggatttca cagtcagtgc catccggctt accgggaaca
     7981 tcattgcttc ggaaggagcc ctggttgccg aaataatgtc accctcggga gccgtggtct
     8041 tttctgcttc ggttgaagct cccggagagg tgcatattga caaatccctg ccggttgagg
     8101 agggagaatg gaggatgcgc tacttcagtc tgaatggcaa aggagttatc cggatgcacc
     8161 ttaaccttgt aagataatgt tgacagttgt tacagtaatg ccggatcaag agtttacttt
     8221 tgttaaatta atcccggcaa accgcagtca cagataacag atacacgatg acacaatcat
     8281 tcatgaaacg gttaaacggg ctcatcccgc tgccaaagct ggtgagattc gtgatcggca
     8341 tctccgtggc agcccagatg atcgtcataa tctacaacaa cttcagcgga ttctacccac
     8401 tccagaactt cacccatttt ctcctgcgcc tcacccgcgg cgtgcttctt acagtccctg
     8461 ctgcaatgct cgtcgctgtt cctgacctgc tgatcatcgg cagacttaac gcccttttcc
     8521 cgtggcacag gagggtcttt ccaagggtgg tgatacagtt tatggcagcc gtcacacttg
     8581 ctgccacggt gtcggtgctg atgaccctga ccgccaacag cctcacccca tacagcgaac
     8641 cgctgaacct cgtgcttctc tataatgccc tgatctatgc ggtggtgaat attatcatca
     8701 tgggggtgct cgaggcctgg gtcttttaca tccgcagcgc agaggcagcc aggaaagccg
     8761 aggcacttga gagggaactg tcacagatgc gattcgagat gctgaaaagc cagatcaatc
     8821 ctcacttcat gttcaacagc ctcaatgtgc tgtccggtct tatagaaaag gatgtctcca
     8881 gggcccagga attcatcgat gagttctccc agatataccg gtacgttctt gaaactgtcg
     8941 agcagcatac aacaactctt ggcaaggagc tcgaattcat gcgctcctac ctctttctcc
     9001 agcagatgcg ttacggggag gacctgacat ggtcggtcag cgtccccggt gacctgctcg
     9061 gcgctgtgct tcctcccctc tcactgcagg tggtccttga gaacgctatc aagcacaata
     9121 tagtaaacca ttcaaaacct ctgcatatcg ggataactgc cagtgacatg atgctgactg
     9181 ttgcaaaccg gcttcagccg aagatctcag caccggcatc caccgggctg ggactgaaga
     9241 atctcaccag gcggtactcc ctaataaccg gcaggctccc gcagtttgcc gttgagaatg
     9301 atcattatat tgcacggttg cccttgctta atcctgaagg taatgatagc gctgataatt
     9361 gaagacgaaa gccttgcggc tgagaagctt gagaagatgc tcgcggaaat cgatccgtca
     9421 gtgactgtaa cggcgaagct cgggtcgatt aaggatgcgg ttaaatggct catgcagaac
     9481 cgggctgaca tcatcttcct cgacatacag ctctctgacg ggatcagctt cagcatcttt
     9541 gagcaggtgg ccgtcaacac gccggtgatc tttaccacag cctatgacca gtatgctgtc
     9601 agggcctttc agcttaacag catcgcctac ctcctgaagc caatccgcag gagcgaccta
     9661 gcagagagtc tgaataaata tcagtcgcta cgggccgcct tcagcatcga ctttgatgca
     9721 cttatggcac agctgcaggg acgagatccg ggttacagga agcgtttcct ggtacagaca
     9781 ggagagaagc tcaggaagat cgaggtcaca gagatagcat ggttctatgt gctggaaaaa
     9841 agtgcctggc tgaagacttt tcagggtaac tcatatccgg cggagctgag cctcgaggcc
     9901 ctggaggctg tccttgatcc ctccgttttc ttccgcatca acaggaagta cctcgtcagc
     9961 atgaattcaa tcgccggaat gacggcctgg tcacgcggcc ggatcaaact ggaactgaag
    10021 ccaaaagctg acgatgaact tgacaccatt gtgagtattg accgctcctc agctttcagg
    10081 aaatggctta acgcctgaaa ctctttctcc ttttaatgaa gtaccagaag agataaggta
    10141 catgcatcac cacgtttatc agcccccagt accagactgc acgttcactc gcaaaggctg
    10201 atgaatccat tatcggggta aatatatcat aggtggcccg tatcaggata tgcaccgcga
    10261 gagtataggc acgcgcctgt acatagagct ccggacggcg ctttattatc gggtaaatct
    10321 cggcagcaag caggatgcat agcgaactgg ccgcataacc gggattctcc gcatatacga
    10381 aggctatgtt ccatgtggtg tagaggaagt tccacatcgg atcggtatac gccagcatgt
    10441 cccagggctt cccttttgag aacgcccatc cgttccgcat ccccttcgac ttgaagagag
    10501 gcatcgtaac gataaggata aatcctgata tggcgttgaa atagtttcca agggaaagat
    10561 ccttgagcga tgcctccaga atgttcaatc ccagcaccgc atataatgcc cataaaaccc
    10621 agtccttcct gaagaatgag agccagcccg gacggtcatc tgcatgagcc agtcgcccca
    10681 aacccaggat cagtgccgta ggtaccagca cggagaaggt tttaacccag cggaaccatc
    10741 catcgagctg agctgcccac agagggaatg tgaatagtgc caggatccag aaaagggctg
    10801 atgccttgta atacttcctc atcagctcca caaggatcaa caggatgcct atatataacg
    10861 gcatcgaaac aaggtactgc cagacaggta tgttcataac gtattattta tagatgaata
    10921 ttcgttcatt aatgattata tgaattacat tttgatcatg tcccaggcaa gctggaatgc
    10981 cttctcaatc cgttccgggg tcattttata ctgctttgtg acatgttcgt cggccagttc
    11041 acggacgaat ccgatcatgg caaggaaaag catttcatca tcaatatcgt tctttatcag
    11101 catttccctt ttgcctcttt tgatcacctc atagactggg tgtttcatgg cctctgccag
    11161 ggcgttctgc tcctcggtta tatacggtga gcggtagtac tgttcaagga agatactctc
    11221 ggagtgatgc tctatccggt gagtcaggta gttgacaaaa accttcttga gacctatccg
    11281 gaacggctcc accggatcat aaccctgcat gaacctgtca agcgacctgt tgcggtagta
    11341 gaggtacatc ctgtggaaga gctcttcctt gctcctgaag tatatgtaaa gagtgcctgt
    11401 cgccacacct gacctggcag ccagcccctt catcgtcagc cctgcaatcc cctccctgct
    11461 tatcagctca agccctgcct catataccag ctcaaccttg ccgtaatcct ttgatctcat
    11521 accacaaaat aaatgaacat aaattcattt tacaatgaca attcgcatgt ttttataatc
    11581 tgttacaaga gaggaatatg agaggccctg tctgtccggt ttagacattc gcaccgggga
    11641 atatgcgtca acccggctgg taattgttcc cggacatcat aggctgaggc ctctgattta
    11701 tattgttttt tcgctatatt gcagaaggca aactcattca ttcctgcatt ataaaccata
    11761 tggaacatat cgtctcgcga accttcgacc tgcttgacag gtacagggaa ctatacaaca
    11821 aggctgatgc cctctgcttc aaacataacg gcaactggca aaaatacagc acagccgaat
    11881 acattgaaca tgcatacagc ttctgctatg gcctctgtga atcgggattc cgcaggggcg
    11941 acaagataat caccgtctcc gccaaccgcc ccgaatggaa cttcaccgat atggggatgt
    12001 caatgcttgg cgttgtccat gtgcctgtct ttacctccct ttcagcaact gaatatgaat
    12061 acattataag aaattcgggt gccaggatga taatcatatc ggacaaaaag ctctgcagga
    12121 ccctcgaacc cgcccttact gccacaggcc actcatgccc cgcattcacc tttgataaga
    12181 tcgacggcgc cgccaactgg ctcgatatag ttgaaaaggg gaggaattgc tccgccgaaa
    12241 cgaaacaaca agtggaggag ataaagaaac tgatcaaacc cgatgacttt gcaaccctca
    12301 tctatacctc cggcaccacc ggcaaaccca agggggtgat gctctcgcac cggaacatgg
    12361 tcagcaactt catctcggca gcatcggtgt tcaacctgaa accgacagac aaatacctca
    12421 gcatactgcc cctgtgccac gtcggcggac gactcggcaa ttaccagacg caattcagcg
    12481 gaacaagcat ctactatgca gaaagcatgg ggactatctc catcaacatg aaggagataa
    12541 agcccgacgg ctttgatgcc gtaccccgcg tactggagaa attctacgat gtgatcatct
    12601 ccaaaggcaa gagcctcact ggaataaaga aatccctctt cttccgggca gtgaacctgg
    12661 gcctgaatta ccagccattc ggcgaaaacg gctggctcta cgaaagaaag ctcagaatcg
    12721 ccgacaagct catcttcagc aaatggcgcg aggcgcttgg cggaaacgtc cgcatagtag
    12781 gatgcggcgg cgccagcctc cagccacgcc tcgaaagggt attctgggca gccgggataa
    12841 agatcataaa catgtacggc ctgacggaaa cttcacccat catcacaatc aaccggacag
    12901 aaaagggaaa ggtgaaactg gggtcggtag gcatgaccat cgaaggggtt gaggtgaaga
    12961 ttactgacga cggcgagata ctctgcaagg gacccaacgt gatgctcgga tattacaatg
    13021 atcccgaact gaccgcctcg gtattcgatg aggagggatg gcttcacacc ggcgatatag
    13081 gccatatcga agagggaaaa ttcctgatgg tcacagacag gaagaaggaa atattcaaac
    13141 tctcaaacgg aaagttcatc gcaccacaga gagtggagaa catctttaag gaatcacccg
    13201 ccattgacca gattatggtt atcggtgaac atgaaaaatt cgccagtgcc cttatctctc
    13261 caaacttcaa atacttcgaa gactggaaga cgtcacggaa agtaaactat tcgagtaatg
    13321 acgaacttat caccctcccg gaagtgctgc aattcttctc ttccgaagtc aataagatga
    13381 ataagcgact cagcccgccg gaaaggataa accggttcag gcttgtaaaa gatgaatgga
    13441 gccctgccac aggcgagctc tctccgacac tgaagcttcg cagaaaattc atccacgaaa
    13501 aatacagcga ggtggttgaa caggtttata acaagtaacc ggaccggcaa caaaaatcac
    13561 acacatgaag ctcatcgagg tcactgacag gaaaacggaa aggatgttcc ttgacaccgc
    13621 aagggtcatt tacaggaatg atgatacgtg ggtctgtccg ctcgacaggc acataaacgg
    13681 cgtcttcgat cccgctgtaa atccctattt caaacacggc accgccacca gatgggtgct
    13741 caccggcgat gacaacagcc tcatcggacg catagcagcc tttatcgact tcaacattgc
    13801 aaaaacctat gaccagccta ccggcggcac aggattcttt gagtgcattg atgatgacgc
    13861 ggcggccggc atgctcttcg ataccgccag ggaatggctc cgcgaacgag ggatggaggc
    13921 aatggacggc cccataaact ttggtgagac agataaatac tggggtctgc ttgtagaggg
    13981 atttacacat ccctcgttcg agatatccta taacccgcca tattaccagg ggctgtttga
    14041 aagatacggt ttccagacat ggttcaggca ggagggtttc cattatgacc ttaccaaacc
    14101 gctcgacacg cggttccgta agattgcaca gtgggtggcc gaaaagccgg ggtatgaatt
    14161 caggcacttt acctggaagg aacaggacag gtacgtcgcc gacttcgcgg aggtgttcaa
    14221 tgaggcatgg gcctctttca aggccaactt cgaacccctc gagccggact atataaaagg
    14281 caccctcaaa aaagccaaag ccatcctcga tgaggagttc atctggctgg cctatttcga
    14341 ggggaaaccc atcgccatat accttatgtt ccctgacctg aaccagattc tcaggcacct
    14401 taacgggaag ctcaacctgg tttcaatgat caaattcctc tggctgaaaa agcggaatac
    14461 aatgacaagg gcgcggggca tgctcatggg ggtgatcccg aaattccaga aactggggat
    14521 cgagtcggcc ttcatcctta aggtacgcga ggcaatcgaa cataagcctc attataagga
    14581 ggttgagttc tcctgggtgg gcgatttcaa ccccaagatg cgcaagattt ttatatcggt
    14641 cggaagtgtt tcagtgaaac actacattac atacaggtac ctgtttgaca ggacaaaaga
    14701 gttcaaacgc tatccaattc cggaaatcaa atgaagtatg acgtaatcgt tataggggcc
    14761 ggcctgggcg ggctgaccgc aggcgcgaag ctggcccgcg aggggatgaa ggtgctggtc
    14821 attgaacagc atagccagcc cggtggatgc gcaacttcct tcaggcgcgg tgatttcacc
    14881 ctcgaggtgg ggctgcacga gatggacggc ccgggcagtc gtgacatgaa gaaccgcatc
    14941 ttcagtgagc tggatgtcac gggcaatgtt gaatttattc atgtacctga gttctaccgg
    15001 ttcatcaggg gtgaccttgc cgtgacaata ccccatgatc cacaactggc cgctgcacgt
    15061 ctgacagaga tgttcccgga agaaaaggag gggatagcga aatactttga ccagctgctc
    15121 agtccgaaaa agaagccggc aggggagacc gaacctgata aaagcgtcgg atcgtttctt
    15181 gactccatta tcagcaatga tgacctgaaa ctaatcctgc tgggtaacct gggctatttc
    15241 catgatgatc cgtattcgct ttcgctgata tattactcag tcgccgaagg gagctattat
    15301 accaacggtg caagtttcat aaagggaggc tcacagcggc tctctaacca cctggccgga
    15361 tacataagga gccacggggg agaggtgctg ctgaaccacg tggtcaccgg ggtgacggta
    15421 gaggctggca gggcgacagg ggtcaccttc cggaaacgtg gaggtaagtc acagaacccc
    15481 gaggaggcca cagctgatga gatagttgca aactgctcca tcccggcgct tgctgatatg
    15541 ctgccaccgg aacagggagc actgcttcgt gaagaataca gcgggctgaa cacaggcgcc
    15601 tcgctgctga cggtctattt cgggtttgac aggccactga aggagctggg tcacagctac
    15661 tattcggcat ttatctatga ccggtcagtc agatctcagg ctgacattgc cgccaataac
    15721 agggatgatt tttcaagacg cagcttcacg tttgtcgatt acggccaggt cgactccggc
    15781 ctggcgcctg aaggaaagag tgtaggggcc atctgctgca ttgactacct ggccgattgg
    15841 gaggatctcg gcagtgagga gtataaactc aaaaaggaat atgttgcgca gacgtttatt
    15901 gaccgccttg gcgaggtgat tccgggcatc cgcgacatca tcggttatta tgaagtcggc
    15961 acccctgtca cggtgaagag gtacaccctc aatcccggcg gggccgttta cggatttgca
    16021 cagaatcccg gcaggaaggt gctcgattcc ttcgctccgg ttgagaacct ccacttcgcg
    16081 tcagcctggg gaagaaccgg cggcggcttc tccggagcaa tctgcggcgg ttatctaagc
    16141 gctttcaacc tgctgaggaa aagggccaga cactgagatt gtcagtcatt tgtgagagtg
    16201 atcgccttat tcaggacatc tgtgatcatt atctccagga acctctccgg ctcctcgaac
    16261 atcgggctgt gggctgaatt tttaaaggtg taaaatcttt tcagcggact ctccagctta
    16321 ttgcagtact cctcagacag gtcatggcta acagtaaggt catgcctgcc gctgatgaaa
    16381 tatgcgggaa tcccaagctc aggaaccgct actgacatat cgagtttcag cacctctgcc
    16441 ctcagccctg atttattcac aaacgaaaac ttcgaacgcc agatattgaa cttttccctg
    16501 atggtatatg ccctgcagag ccagaccgga taaaaaaccc cggtcattat tgagttcatg
    16561 tttctcatgg ttcctatacc cagttcgtgc atcgatttgt cacgtaacac ggaattaaaa
    16621 aagggcagga gatatgcttc atcttcaata atcgggtatt cccggaattt tgctgccatc
    16681 tttctgtttc ccgaagagga atagctatca accatatatc tgtacgccag cttttcagat
    16741 tcagcctgtc tggttatctg tgccatcccg atataggcat ggtacagttc aggattttcg
    16801 gcaactgccc tgagggcgaa agccgtacct cccgaatgag ccagaatata tatcctttcc
    16861 ttgccgaaac ggctgcggag gtagttcgtc acctcaatca tatccgcggc aagctgcccg
    16921 aggttcaggc tctccattgt aacttcgcgg gtagcggaga gccctccgcc ccgctgatcc
    16981 cagtaacaga cagtgaaatg atcctccagc cctgtcgggt atttttctgc aaggaaatac
    17041 tcggagaagg agggaccgcc gtgcacaaac agcagtaccg ggttggctgt gtctgcgccc
    17101 tttataaaca tcccctggcg gacgccgccg atctcttcga atctcttttc acagatgctg
    17161 ctgcctgaac ccgcagcggg gcaggagcgg gttttcccgg gacttaccag gaccagcatc
    17221 agcagcggca tggcaacaat aatcaataaa gcagtaatga caagtatcag catatggtgg
    17281 gtttttgctt ttaatctgtt catgacataa agatcataat aaattctgtc tgcaccggat
    17341 gctgcacaac cttaaatatt gcaaaagatg aagcagtttc tccgggctcc tgcaaacaaa
    17401 cacaaatcct cattgttatt gctattgaag taccattgtt tctaaaacac cattgttatg
    17461 acaccgcacg ctgaaaaact ggttattacc gtatcagata cggtaaacgc cccgctccag
    17521 cgggtatggg aactgttcac tgacccgctg catataatac actggaacca cgcatcggac
    17581 gactggcaga cgagttatgc agagaatgat cttcgccccg gaggggcatt cctctcaagg
    17641 atggaagccc gggacggttc tgagggattt gactttaccg gcacatacag cgaaatcgta
    17701 ccgggtaagt caattgcatc cacccttgac gacgggagga acgttcacac aagcttcagc
    17761 gagaaggcag gagtgacgct cgttactgag tcgtttgagg ctgaggatca gaacagcgcc
    17821 gagctgcaga gagccggatg gcaggcaatc ctcgatagtt tcaaaaggta tgtggaaggc
    17881 tatggcccca ttgaaaagct ccattttgaa atactcatta acaactcccc tgagaatata
    17941 ttccggatca tgacaggcag cgatacatac aacaagtgga ccactgaatt caatcccaca
    18001 tcccggttct atggatcatg gaataaaggg gagaagatca cattccttgg tacggacgcc
    18061 gacggtaaga ccgggggaat ggtctgcagg atcagggaga acatcccggg gaaattcctc
    18121 agcatcgaaa gcctcaggga gttcgtcgac ggcagcgagg acgccggcac cgggaaagaa
    18181 aacagctgga gcggggctct ggaaaactat acgctgaaac cggcaggaag caaaacactg
    18241 ctgtgcatcg ataccgatgt cccggcagaa cacagaccgt ttttactgga ggcctggcca
    18301 aaagctttgg agacgctgaa ggagctttgc gaataaataa atgtgacgaa gactcttctc
    18361 agccttctca ggatcgatgt ccctgtcagg gactgaaaca atacattact tcagttgtta
    18421 ttacaattgc attctggcaa ttttcttcga aatttctgat gcaggatgtt ccgctttttt
    18481 ttattctgcc cacgtgatct gaattaaacc gaaaggagta aaccatatga acgcactcag
    18541 aaaaacctct ttgatcttcg gtctcttctt cgcaggtact tttgtgttct cgataccggc
    18601 actattcttt tatgacccgt tgctgcacga ctccggctac attcatggcg gggggtttga
    18661 caggcgtgct tcaatgggcg cccttttcga gatactgact gccatctgca acatcgcgac
    18721 cgccatcgtc atcttccctg ttgtaaaaag ggtcagtgaa tccgccgcag tcggttacat
    18781 cacatcacgc accgtggagt cggtaatgat tctctcaggt gttgtcagcc tcatggctat
    18841 tgtcacatta cgggccggct tcgagcctgg cggtgacacc gtggccctca ccatgacagg
    18901 tcagggcctc cttgcatttc atggctggac ctttctgctt ggaccacaat tcggttcagg
    18961 attcggtaac ggaattctgc ttggatacct tatgtaccgg tcggggctcg tcccccggcg
    19021 tatggccttg ttaggcctca ttggaggacc actcgccttt gccggtggcg tgctggtcct
    19081 gtttgatctc cttaagccta tgagtgccgg actgtttgct cttacaggaa tggaaatcgc
    19141 ctgggagctt tcgattacat tctattcact tttcgttggc ttcagaccga cacaggaaaa
    19201 atgaaacaag atggaaaaaa tgaaagcagt ggtgtatgaa aaatacggac caccagatgt
    19261 cttgaaactc actcaagttg agaaaccagt ccccggggac gaagaggttc tgataaaaat
    19321 caatgccaca acagtgacag ctgcagactg gagactacgg aaagctgatc cctttcttgc
    19381 ccggattttc aatggactgt tcaaacccgg aaagataaaa atattggggt ttgaattggc
    19441 cggtgagatc gaagagaccg gaaaggatgt caggctgttt aaaaaagggg atcaggtgtt
    19501 tgctgccacc ggatttacat ttggcgccta tgctgagtat aaatgcatcc cggaaggggg
    19561 aactattgcc ttaaaacctg tcaatatgac ttttgaagag gctgccgcta ttcctgtcgg
    19621 aggactgact gcattgcact ttctcaggga caaaggacat atccggggca gatccaaagt
    19681 gttgatctat ggtgcttccg gcagtgtggg aacttatgcc gttcagcttg cgaagtatta
    19741 tggggccgaa gtcacggcag tatgcagcac cccgaatgtt gagcttgtta aatcccttaa
    19801 agctgataag gtcatcgatt atacaaaaaa tgacgtcagc agggaaggtg aattatacga
    19861 tctcattttt gacgcggtgg gaaagatttc attgtctgcc tgcagaaaaa tcatgaaacc
    19921 cgggggaaag tacatatctg tgagaagagg taccgcaaaa agcagggaag gagacctggc
    19981 atttctgaaa aagcttgttg aatcaggcac tgttaaacca gtaatagaca gactgtatga
    20041 ccttgcccag attgcggaag cacatgccta tgttgaacag cagcataaga aagggaatgt
    20101 tgtggttact gtaaaatgag caatcgcagg caattacagt tcttttgaaa ccaatgctgc
    20161 caggatgcct gaaagtctga cagtctgtgc acacctttat ttccttacag gtttaggccg
    20221 gattccgata atattcggca aaggcagcat caggtcagga tcctccgaaa ggatcagctc
    20281 cgtgctgccg gccttcaggt atacctgtgc cggggcattg ccctcgagat gctgcgcggc
    20341 cacaatttcc atatcagagt aaaggagaat cttgttgaaa tcatgcatcg agaccggcga
    20401 aggacagtaa aggaatagta tccgtccctg cttgtcttcc gcgatggcag cttcactcca
    20461 cctgttgccc tgatcaggcc agacactgat acccagccgc ttgatcagcc gcaggttctg
    20521 gatgacagtg gagtaatcct taagaacgct gtcgattgtc acccctggct gatcgaggtc
    20581 aaagatcctc gccgggggca cctgctttcc tttcgcggga ttgaaggcaa ggagcgattt
    20641 ataattgttc ctgtgaagac tgctgcggtg ttctccgtcg cgcacatatc cggtgtgggt
    20701 ctgataatca tcgttgtaca tacccgcgtt gatggcagcg gtcaggctgt acttttcgca
    20761 ccactcctta ggagtcttca acggcccgtc agcctcctgg ctgattcccc tgaaaaccag
    20821 gtcccatttc tgcagatcaa tcctgattgc attgatctca atagggtagc tgtactccgg
    20881 cagtgtcacg gactgtgccc ggtattcaag tccaggggat aattctttcc actgggcctg
    20941 aaccagggac gggataagca gtgccagaag gcagataata tatcgcatgg atctctttca
    21001 gtctttagat taagaacgtt attatgtgca aatcgttgtg caagagatat gcaatatctc
    21061 gccttaccgg aaggtaactt accaagatgt aacttacaaa attgtaagtt atcatatttc
    21121 ctttgcattt ataaaaaatc atggaaacac aatttgtttc cggcacagtt gcccctgtta
    21181 tgaatttctc tgagaggagg caagagacag caatgaggag agattaaatg ccggcgtgga
    21241 acgttggcgc aaactgagac accagttatc tcctgttccc tgttaatgaa atttggctca
    21301 tcaactggta ttccatacct gaatggatta ttcatttttt tcctgtggat tgttatacaa
    21361 tacagaaata cgcagcttgg cataacgcac tattgcgtaa ttgattttta tgtcgtgaag
    21421 acatgaaaaa cccggaatac ctgatgcagc tcaccggacc cgtcaggccc ctacttcaat
    21481 tccacatcca ggtacaccga gtgcctcccg taagtgtcaa agaatggctt gtatacaaca
    21541 ccatcaatca tgaactccag cttcgaggga tcaccggtaa ttgacttgct gatgtctgaa
    21601 gcatccagcg tcaccttgcg ccactcggtg cgcggagcag gctcctgcgc tgccagcagc
    21661 accggcccgt agaacaggct cgcaatgttc tgctgatcca tcaccggctc aaggtaaaat
    21721 ttgaacggca tccgcagctc aacagtatcg cccttcttcc acttgcgact gatagtcata
    21781 tagctccccg gcaccgcatc aacactaaaa tccctgccgt tgatcttcac taagaatcct
    21841 ttcgttgccc agtgcggcac ccgcacgttc aggtcaaacc tgcccttgcc gccaataacc
    21901 agctgagtgc ggtcttcctt cgggtaagcc gtggcttgcg taacagtgat cttcctctcg
    21961 gtccatgtca gcgtcgaggg tacatacagg ttcacataca gcgccctgtt atcggcactc
    22021 ctgaagtaaa tcgagttctg cagcttcgtg ctgctctcca gcgccgtccc gttgcagcac
    22081 gagaacccgt ccatatcctc gttcccgaac tgcttcatcg agcccggcct cagaggcaca
    22141 tgataggtgt tcccggcatt atgttccgcc accgaggcaa ggatgtgatt gtagagcccg
    22201 cgctcatagt aatccatcag cgccgcatcc tggttgaaga ggaagaggc
//
LOCUS       DAOPXU010000042        21869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_2430_length_21869_cov_13.936600, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000042 DAOPXU010000000
VERSION     DAOPXU010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21869
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2430_length_21869_cov_13.936600"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /gene="gldN"
                     /locus_tag="PKK03_05930"
     CDS             <1..717
                     /gene="gldN"
                     /locus_tag="PKK03_05930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HNX83973.1"
                     /translation="VIWSKRLYRIIDLREKMNLNLYYPSLKYDAAQNRFTSEMSDGRM
                     NFSGILLNEILSGRVPATDGDAMTVPTTYNDIAAKMNRDTLTIAVVGADGLEHDSTVT
                     NMENPADIKQLMLLEEWFFDKKHSRLDVRIIGICPIYMGYDPITSRLVKRRLFWIRYE
                     DVRQALATSEAFNSFNDAQRISFEDLMLQRRFNGYIVAESNVYDDRSINQYKMGPAQI
                     FEAERIKNEIFNFEQDLWEY"
     gene            complement(880..1965)
                     /locus_tag="PKK03_05935"
     CDS             complement(880..1965)
                     /locus_tag="PKK03_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNX83974.1"
                     /translation="MKNRYLLIMAGGVGSRFWPLSRREKSKQFLDILGTGETLIQMTY
                     RRFADICPPENIYVVTNEDSSDIVVSQLGIDPDNVLAEPLRRNTAPCIAYGVFRIMAE
                     NPDAVIAVAPSDHLIRKQDEFRNVMDEAFRFASENNALLTLGIRPDRPETGYGYIQAN
                     TMQPVIGYEGLHKVKAFTEKPGLAMAKLFLESGDFYWNSGIFIWRASTILESYSRHLP
                     DIFHTFNDGKALFSTADEREFITQAYSQCSSISVDYGIMEKADNVYVICTDLGWSDLG
                     TWSSLYLHSEHDRNDNAVLNSKAFTYNSEGNIISLPQGKVAVIQGLDDYIVIDSGDVL
                     LIVSREQEQNIKLYLDDVGREAGEKFL"
     gene            2080..2823
                     /locus_tag="PKK03_05940"
     CDS             2080..2823
                     /locus_tag="PKK03_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX83975.1"
                     /translation="MFRFLSGFGSYWMLLGRVFAKPEKHSMYYRQTMKELVSLGLRSI
                     GIVTIISFFMGAVITLQTAYNTENPIYPTYLIGLGARDSIILEFSSTIVALILAGKVG
                     SNIASEIGTMRVTEQIDALELMGINSASYIILPKVVATVIFNPFLTLISIITGIIGGW
                     IVGTSAGVITSQDYIYGIQYAFIPYYITYSLIKTVFFAFIISTVSAHQGYHVEGGSLE
                     VGRASTKAVVYSSVTILLFNVILTQLLLS"
     gene            2820..3575
                     /locus_tag="PKK03_05945"
     CDS             2820..3575
                     /locus_tag="PKK03_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX83976.1"
                     /translation="MIRAEHISKSFGDRRVLTDISAVFEPGKTNLIIGKSGSGKTVFL
                     KCLVGLLDVDEGEIYYNDVPFTRLDFRGKKELRQKMGMLFQGSALFDSMTVEENVRFP
                     LDMFTGMSYPEKLDRVNFCLDRVNIVNSNRLFPSELSGGMKKRVAIARAIALKPRFLF
                     CDEPNSGLDPKTSIVIDELIREITVELNMTTIVNTHDMNSVMENGEKIIFIEEGEKCW
                     EGNRNEILKSDCSKLNDFVFANSLARQLKDSSK"
     gene            complement(3584..5038)
                     /locus_tag="PKK03_05950"
     CDS             complement(3584..5038)
                     /locus_tag="PKK03_05950"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HNX83977.1"
                     /translation="MKRKILALIVLLSAVTAGHSQNSQVLYFMNLPQATTLNPAFKPT
                     GRFYLGLPGISDISVRLDNNFLSLSDLFTSGVISDSTLSFLERGPWLNDFLAGLGDNN
                     SLEPQAGVRLFGLAFTVKDNLRFSLDITERAEANLVFPGDLFRLGLGGNSDFIGKTLD
                     LSSFRTDMMVYHEIGLGASGNITEKLRIGGRLKILSGVASAYLTNNHSKLTVNEDFTH
                     TADADISLNMSAPVLFTTESDGTIHGAEFDEARFDDTRNAISYLKELANPGAGIDLGA
                     EYRFSEKLAVSAAVNDLGFIRWKRDLSQLTINSTFKFNGLTMQDVYDESLDFEELINW
                     TIDSLQNVLELADSPQEYTTYLPAKVVAAFSYTPVRYFTLGLLSQTRFEGPQAHQSVT
                     LSGNLHFRNIFSTTLAYTAANRRFDNLGFGMALRGGPFQFFVLVDNIPLKYSSFTSGE
                     QTIRLPENMNTVNARLGLNLLFGNRERDKTLPPM"
     gene            complement(5048..7792)
                     /locus_tag="PKK03_05955"
     CDS             complement(5048..7792)
                     /locus_tag="PKK03_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83978.1"
                     /translation="MRKRTGIILVGILAIALAVAAFFLRKEKQVVVIDPWEAVPADAF
                     FVIETSDFPELLTRITDPAGILSGLSGLDWAASLVRSASSIDSVTGGREVRELISNRR
                     MLISFHAAGQRQTVPLAVMSTGPAFTARKLTLLCSQAGATVTDKRDLGGTRTFTLTFT
                     RGSRQKPVYLALTSGILIITPSESMITAALDNRSAGSDIRHQQGFTQVVSAAGKTADN
                     LFVLFRNLPPFLKSFTDPEDISVISSVAIAGGGDLTTAEDGLFISGFLTTAGAGTGAD
                     RLRDVAPAENGVHELLPRNTLSYSTVMRRASLSGETATDPASINATDLALILSPFTGT
                     EVTEGLIAFGEGSVRIRLFRMTDPQSAEEVLRQRLAAKYRSMGLRESHFVASARNSDG
                     AEEVIYRMPFTGVASILAGAEKSRAGDEWVTFARSYMIFSQSPEALAAVLRESAGGNT
                     LINDPGFREMEKSLPTKSSFLFYSSGRVLKSQLAEYLTPAAAASLTDRALSGISGVGV
                     SMTPSNEMIYTSLSVRYTSGGEHPGSAPAAAAATEVTTGETDSSGLKLLWKVKLDAAP
                     SVKPFFFTNHNTGATEIFIQDRNNNIYLISSSGKILWKAAIREKITGDIFMIDYYKNG
                     KLQLLFTGRDYMHLIDRNGNYVDKFPVKMQSPASNTLALFDYENNKDYRLFIAGDDRK
                     IYAYDRSGTPVRGWNLFTARGKVTDPVTFFRVRGKDYLFVADDQAVYVLDRTGNIRVA
                     HQEPLMKAPGSVARLTAEDEPSIIFSALDGATVRLLFDGTVKKDTIRGISASHRSEFG
                     DIDGDDVTDRLTVDQGVLRAIDGSGHEMWRYSAGGGEVTGPWIVSTGTDERRIAIYDT
                     GKGMLHLIGRNGASVSGFPRPAGPLFNTGKVSNKSTWNLISVEDEIYLCNHELNSGSK
                     "
     gene            7951..10353
                     /locus_tag="PKK03_05960"
     CDS             7951..10353
                     /locus_tag="PKK03_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX83979.1"
                     /translation="MNKFTFLLLFLAPLMSLSAQTVTVLSADDGKPIADVAVYNEART
                     QFGYTNYSGKVSLSGFSGEQPVFFQHFSYERVSFTTEELMAAKWVVKLETKTFEVEEF
                     IVSANRWEQKSEEVPNHINVVALPAVKIMNPQTAADLISMGDVFVQKSQLGGGSPMIR
                     GFATNRVLINIDGVRMNNAIYREGNIQNVISIDPNIIERTEIIFGPGATMYGSDALGG
                     VMDFHTRRALFSTGDSLFVKAEAMARWSSAAGEQTYHAWINAGGRRVALLSSVTWSDF
                     GDLVMGSVSHSEYLRDLYQERIGNRDSVIMNSNPRRQVASGYRQINTTNKLRFKAGKH
                     LDIDLAHHYSHLSDVPRYDRLIQVKGGTLRYGDWYYGPQVWMMTNASMRYNRPANLFD
                     EIRLTVARQDYRESRHDRALNKTDLNEQFEKVGIWSANLDFDKKTGKRDNLAYYGAEY
                     VWNTIRSTADVKNILTGETEPAGSRYPNGKNIYNSFSLYGGYKFNLSSKFTVSTGARY
                     NYVSLNSEIADNSWYNFPFTTIESGNGALTGAAGAVYRPDGRTELTVNLSTGFRAPNL
                     DDLGKVFESAPGVLVVPNPDLRPEYLYNIDLGASREFGKMLLAEASVFWSYLDNAMVR
                     REFLFNGEPTFVFQGEESQVYAMVNAGYAIVYGTQLKAELRPSSFIRVKSSLSLTAGH
                     DDEKAPLRHVPPLFGATHLIYERSVLKADLYAVYNGSITNDRMAPSEKDKPYLYAEDE
                     NGNPWSPGWFTLNFKTSWNISNRLELTAGIENLLDLRYRPYSSGIAAPGRNFIVSARI
                     KV"
     gene            10547..11731
                     /locus_tag="PKK03_05965"
     CDS             10547..11731
                     /locus_tag="PKK03_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83980.1"
                     /translation="MNRGYRLAELSSKKDIRDFHDLPAFINREDKNWIKPLFKDIETI
                     FHPSSNTLFRTGEAVRWLLRDDTGHAVGRVAAFYSKKIASAHGEQVGGMGFFECINDR
                     EAAAILFDACRNWLEEKGMKVMEGPVNFGDRDRWWGLLVDGFMPPNYCMPYNPPYYRE
                     LFEAYGFRNYFDQYTYHMPVDDSRLDAVIREKAERIFSNPDYTFRYMTLKELRDISRN
                     FMIVYNKGWARFPGVKTISEGHAKLILKSIRPVLDEKLLWFGFYRNEPICFFIMLPEV
                     NQIVRHLNGKLNHVGRLKFLWYRHIKNELTKAFGLIFGIVPEHQRKGVEGALIMSFAR
                     LAMSDRFPYKELEFNWIGDFNPSMMHLLDQIGAKIIKTHITYRYLFDRDAPFERAPVV
                     NA"
     gene            complement(11721..12716)
                     /gene="trpS"
                     /locus_tag="PKK03_05970"
     CDS             complement(11721..12716)
                     /gene="trpS"
                     /locus_tag="PKK03_05970"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNX83981.1"
                     /translation="MDTVVSGIRSTGNLHLGNYFGALKNFLRMQDENNCFFFVADYHS
                     LTTHPHPDDIHGNIRQVLAEYLAAGIDPEKATVYVQSDVPEVTELYLLLNMISYVGEL
                     ERTASFKEKIRKHPDNINAGLLTYPVLMAADIIIHRAHKVPVGKDQEQHLEMTRRFAR
                     RFNTMYGVEYFPEPDAYNFGRQLIKIPGLDGTGKMGKSEGNGIFLADTPAEIRKKVMK
                     AVTDTGPEKPGAEPSEPVKNLFTIMEVVSESSTLQHFREQHATCQIRYGDLKKQLAED
                     IIRVTDPIRIRINEILNDPDYLGKVVASGAEKARASASKTIREVREIIGYRPFTH"
     gene            12840..13178
                     /gene="rbfA"
                     /locus_tag="PKK03_05975"
     CDS             12840..13178
                     /gene="rbfA"
                     /locus_tag="PKK03_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNX83982.1"
                     /translation="MESTRQKKVARLVHKELAEIIMHHGTELAPGKMISVTTVRVSPD
                     LSLAKTWISVFPSEGSAATLEVLRTSVPRLRYELGQKVRNQLRIVPEIAFFIDDSNDY
                     IDNISKLLKE"
     gene            13184..13999
                     /locus_tag="PKK03_05980"
     CDS             13184..13999
                     /locus_tag="PKK03_05980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX83983.1"
                     /translation="MHYDPIKDLLGQVFNRHPLLRKLFYRLLNILLLRSWHVRRALKN
                     LGPSLPDSANVLDAGMGFGQYSWWMASHFTSWQITAADIKSEQVADCNEFFRRTKLAT
                     RVHAVEADLVTWSSVESYDLILCVDVMEHIAEDRTVFANFLKMTAGGGYLVISTPSDQ
                     GGSDVHSDGDTSFIGEHVRDGYAREEITEKLRTAGYTEVSVSYTYGTPGSIAWRLSMK
                     YPVIMLSASKLFFILLPFYYLAVMPFVLALNCADLNGSHRKGTGLLVIARRSR"
     gene            13996..15231
                     /locus_tag="PKK03_05985"
     CDS             13996..15231
                     /locus_tag="PKK03_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX83984.1"
                     /translation="MKLSIYIAKRYLFAKKSRNAINVISAISVAGVTVGTMALITILS
                     VFNGLEDMVRSIFSTSDPEIKITPVAGKVFTPDSLMLKKLASAEGVDVYAMTLEENAL
                     LRYDEQQYIATVKGVSLNYAAVTDLDTAMWDGNFILQGENGNPYAVAGLGVANYLGMR
                     LNFISPLAIYIPDRKAKIRGTPDNEFTRKYIFLSGIFAVEQEFDSKYVFLPLDFTREL
                     LDYTDEVSSIEVRMKPGADEKKTQNAIREAMGDGFRVQNRYEQQEIFYKVMKAERLAI
                     FVILSFILIIASFNIIGSLTMLIIEKERDISILKSLGADNRLIKRIFIYEGWMISFIG
                     TIIGLLLGFVLCAAQQHFGIVKLAGESLLIDAYPVRMRLADFFVVAATVLAIGYAAAW
                     YPVHYLSRRQLKDKEKAIK"
     gene            15228..16010
                     /locus_tag="PKK03_05990"
     CDS             15228..16010
                     /locus_tag="PKK03_05990"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HNX83985.1"
                     /translation="MKTVYKIFVVTVMLMIMSCGDSENEKPSRKMRIPEDKLVRILTD
                     TYLTAGMMDLISMRETWAQRDSIQNYADVIESHGYTYEQFETTMRYYFTGKPRKLSRI
                     YDRVTGNLLELETEVMTQQNAVKPSEKNLWTGKPDYRLPEDFTRDPVWFDIPVDRPGE
                     YILKADIRVFEDDKSLNPRVTVYFSYPDSTGEEKRDYWQEIFLGKDGQIRNVQISHTL
                     DSIPGSRLRGWLLNHDNQPGKWEKHARIANIRIVHADDFAVQ"
     gene            16013..17116
                     /locus_tag="PKK03_05995"
     CDS             16013..17116
                     /locus_tag="PKK03_05995"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNX83986.1"
                     /translation="MRRISAQYLFTSAGRPLRRGIVTVAGDGTVTGVEDTGGSLRESA
                     STEFYNGVIIPGFVNCHSHLELSHMRGLIPAGGGLSDFIVSVRDRREASDDEIMAAVR
                     AADIEMRSGGVVACGDISNSPVTAVTKSKSPIRYITFAEVFGLDPAVAEVRMRGALEV
                     CEEMRRRSLQAQVTPHSLYSVSAPLSEMIREQISPDSVISLHFLESDDERKMAQGHAD
                     AALGLSRLASRLLLVHNTVITREEALKVASAGNTWFCLCPSSNMYISGKMPPAAMLRE
                     LTGKIAVGTDSLASTCRLSMISELLLLQEAVPGIPAEELIRWGTINGAMALKCDDILG
                     SIEPGKRPGLLLVEPFDLQNMRLLPESRVRRLV"
     gene            17317..18099
                     /locus_tag="PKK03_06000"
     CDS             17317..18099
                     /locus_tag="PKK03_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX83987.1"
                     /translation="MSTFLFDSIVFGPVHSRRLGVSLGINLLPVTRKVCSFNCIYCEC
                     GWTPGKPGPKEKLPTRSEVRDALRKKLSGMKEQGKAPDVITYAGNGEPTLHPDFAVII
                     DDSLALRDEFFPAAKVSVLSNGSMLRLKSVREALKKVDQNMLKLDSALPDTVRQINQP
                     HVKTDLARYEEYIREFKGRFIIQTLFVRGECDGRTVDNTTEEEITAWLEAISRLRPQQ
                     VMIYTIHRDTPLGNSLRKVPVSELNAIARRVSQLGITTTVSG"
     gene            18114..19265
                     /locus_tag="PKK03_06005"
     CDS             18114..19265
                     /locus_tag="PKK03_06005"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX83988.1"
                     /translation="MKVLISPLSWGFGHAGRMIPLAMELERRGCEVVFAADAPLTEMI
                     VRELPGIKTVEIPGLRIRYSRYLPQYVSIFLQLPVIVASAVREHATLRRLAKELDPMV
                     IISDNRFGFCHKKIFSVYVTHQVRIAFPAFLAFLEPLAAWMHRMIISRYDLCLVPDYR
                     GSENLSGRLSHGGRLPANVAFIGPLSRFGVAGTEPFPPCRMKSGPLSPCRTKSEPLSP
                     CRTKSEPLSLSRAKSEPLQPDTGAPPSSLAQPYACLILSGPEPQRSLLLRKVSESLRE
                     FPLAVLSATPLNTPLPSGQAIHLLTGAETGTMRQVITGASMVIARTGYTSVMELVSLG
                     MGAVLIPTPGQTEQEYLGRHLDGRYGFITMNQNRLERLASVILPEHKKE"
     gene            complement(19242..19934)
                     /locus_tag="PKK03_06010"
     CDS             complement(19242..19934)
                     /locus_tag="PKK03_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNX83989.1"
                     /translation="MNPGDNGLPGNGRGEEESRMHPGNEQWLKRAAEILSVVFHPIFL
                     PLYGLLIIYSAHTLHSFLPPQIKRMILILVMANNLLMPMALATVLYARGAIKTFNARD
                     RNERVILLSFSLLMYSLTAFILLRMQVPGLFRAFFISIAIVTFSTLLITMFYRISLHA
                     SGIGGLMVLVIFMAAVYNISMLWQAVAVALTAGAVMSSRIYLGDHSPSEVWAGLAAGT
                     VIMGLSLFFMLR"
     gene            complement(19987..21450)
                     /gene="rpoN"
                     /locus_tag="PKK03_06015"
     CDS             complement(19987..21450)
                     /gene="rpoN"
                     /locus_tag="PKK03_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNX83990.1"
                     /translation="MLDQKLQQKLLQKLSPQQIQMIKLLEVPAMQMEERIKKELEENP
                     ALEEGPEEDEVQGDEDDDEVDDEQAEREQDEFALDDYLNDEDIPDYRLQVNNASKDDE
                     RREDIPYAAGSSFHENLIQQFNLRTDTEREDLLGEYIIGNIDDDGYLRREVENMVDDL
                     AFLQNITTDVNELNAILRIIQDLEPAGVGARDLRECLLLQIGRKDQSVPSVTLAKTVL
                     TDYFEEFSRRHYEKIMNRMGIGESEMKGIIDEVLKLNPKPGGTISDPYARSAQHIIPD
                     FILEQSDSGLELSLNNRSIPELKINRDYSEMLETYSRDKKAGKQNRDAIMFVKQKLDA
                     ARWFIDAMRQRQNTLLLTMNAILEYQKDYFTEGDDTKLKPMILKDVAEMTGLDISTVS
                     RVANSKYIQTHFGIYPLKFFFSEGMQTDAGDEVSTREIKRILQECLSNEDKRKPLTDE
                     KLTEILQEKGYQIARRTVAKYREQLNIPVARMRKEIK"
     gene            complement(21471..>21869)
                     /locus_tag="PKK03_06020"
     CDS             complement(21471..>21869)
                     /locus_tag="PKK03_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNX83991.1"
                     /translation="RDLQAEHERYLVETHFRRPVILTDYPMNIKAFYMKQNDDGKTVR
                     AMDVLFPGIGEIIGGSQREEELDKLTRRIEELNLPLKDLWWYLDTRKFGTAPHSGFGL
                     GFARLILFMTGMSNIRDVVPFPRTPRNAEF"
BASE COUNT         5230 a         5834 c         5616 g         5189 t
ORIGIN      
        1 gtgatctggt caaaaagact atacaggata atcgacctga gggaaaagat gaacctgaac
       61 ctctactatc cgtcacttaa gtatgatgcg gctcagaacc gcttcacatc ggagatgtca
      121 gacggaagga tgaacttttc gggtatcctc ctcaacgaga tcctttcggg aagggtcccg
      181 gccaccgacg gtgacgccat gacagtacca accacgtaca atgacatcgc tgccaagatg
      241 aaccgtgaca ccctgaccat cgctgtggtt ggagcagatg gactggagca tgactcaacc
      301 gtgaccaata tggagaaccc tgcagacatc aaacagctca tgctgctcga ggagtggttc
      361 tttgacaaga aacattctcg ccttgatgtc cgtatcatag gcatatgccc catttacatg
      421 ggctatgatc ctatcacctc aaggctcgtc aagagacgtc tcttctggat aaggtacgag
      481 gatgtaaggc aggcactggc taccagcgaa gcattcaact cgttcaatga cgcccagcga
      541 atctccttcg aggacctgat gcttcagcgc aggttcaacg ggtacatcgt tgccgaatcc
      601 aacgtctacg atgacaggtc catcaaccag tacaagatgg gccctgcaca gatattcgaa
      661 gcggaaagga tcaagaacga gattttcaat tttgaacagg atctttggga atattaggat
      721 cgtaatttga atccgtatga tgtagcgaca ggtctgagac ctgtcgctac gtgtttatat
      781 attcccatac ctgcatgcat cctcgtggcg acaggtctga gacccgccgt tacctgcatg
      841 attacagaat tttgtatatg gatgcaggat cctgaattat tacaggaatt tttccccggc
      901 ctcacggccg acatcatcaa ggtacagttt gatattctgt tcctgctcac gcgaaacaat
      961 caggagaaca tcaccgctgt caatgacgat gtagtcgtca agcccctgta tcaccgccac
     1021 ctttccctgc gggaggctga tgatgtttcc ttctgaattg taggtgaatg ccttcgagtt
     1081 cagcacggca ttgtcattcc tgtcatgctc agaatgaaga tagagtgagc tccaggtacc
     1141 caggtctgac catcccaggt cagtgcatat aacatacaca ttatctgcct tttccataat
     1201 gccgtaatcc actgagatgc tgctgcactg tgaataggcc tgcgttatga actctctttc
     1261 atccgcagtg ctgaacagag cttttccgtc attgaatgta tggaagatat caggcaggtg
     1321 cctgctgtac gattccagta ttgttgaagc cctccagatg aatatgccgc tgttccagta
     1381 gaaatcgccg ctttccagga agagcttcgc catggccagc cccggttttt cggtgaaggc
     1441 ttttaccttg tgaagaccct cgtagccaat gaccggctgc attgtattgg cctgtatgta
     1501 gccgtaaccg gtctcaggtc tgtcaggcct gatgccgagg gtaagcaggg cgttattctc
     1561 agaagcaaac ctgaaagctt catccatcac attcctgaac tcatcctgct ttcgtataag
     1621 gtgatccgaa ggagccaccg ctatcacggc atccggattc tccgccatta tgcggaaaac
     1681 gccgtatgca atgcacgggg ccgtgttgcg cctcagcggt tcggccagaa cattgtccgg
     1741 gtctatcccc agctggctga caacaatatc actgctgtcc tcgttggtta ccacgtagat
     1801 gttctcaggc ggacatatgt ctgcaaaacg gcggtaggtc atctgtatca gggtctctcc
     1861 cgtcccgagg atatcaagga actgcttgga tttttccctc ctgctcaacg gccagaagcg
     1921 gcttcccacg ccgccggcca tgatcagtaa gtagcgattt ttcataacta ttcttgatcg
     1981 gtctgcactg ccgcaaatat ataaaaacca tcggaggaat accggagccg gctgagaagc
     2041 cacgaattat tgttaaattt gaccatacaa ttattcccca tgttcagatt cttgtcagga
     2101 ttcggctctt actggatgtt gctcggcagg gtgtttgcaa aacctgaaaa acattccatg
     2161 tattaccgtc agaccatgaa ggaactggtg agcctcgggc tccggtcaat agggattgtc
     2221 accatcattt cctttttcat gggagcggtt attacccttc agacagccta caataccgag
     2281 aaccccatct accccaccta tctgatcggt ctcggtgcca gggactccat aatccttgaa
     2341 ttctcctcaa ctatcgttgc actcatcctt gcaggcaagg tgggctcaaa catagcatcc
     2401 gagatcggca ctatgagagt aacggaacag attgatgccc ttgaacttat gggaatcaac
     2461 tctgcctcgt acatcatcct cccgaaggtg gtggctaccg tcatcttcaa ccccttcctc
     2521 accctgataa gcattatcac aggcattatc ggcggatgga tcgtcggcac cagcgccggt
     2581 gtcattacct cacaggacta tatctacggc attcaatacg ccttcatccc ttactatatc
     2641 acatactcac ttatcaagac tgtcttcttt gccttcatca tctcaacggt ttcggcacac
     2701 cagggatacc atgtagaggg aggttccctc gaagtgggcc gtgcaagcac gaaggcggtg
     2761 gtgtacagca gcgttacaat cctgctcttt aacgtcatcc tgacacaact gcttctctca
     2821 tgatcagggc tgagcacata tccaaatcat tcggtgaccg ccgggttctg acagacatct
     2881 ccgccgtctt cgagcctggc aaaacaaacc tgatcatcgg aaagagcggc tccgggaaaa
     2941 ccgtctttct caaatgcctt gtcggactgc ttgacgtgga tgagggagaa atctactaca
     3001 atgatgtccc tttcacccgt ctcgacttcc gtggtaaaaa ggagctccgc cagaagatgg
     3061 gcatgctctt ccagggaagc gcactctttg actcaatgac tgttgaggag aatgtcaggt
     3121 ttccgcttga catgttcacc ggcatgtcat acccggaaaa acttgaccgg gtcaactttt
     3181 gtcttgaccg ggtgaacatc gtaaactcca accggctttt tccttccgaa ctatccggag
     3241 ggatgaagaa gagggtggcc atagccaggg ccattgcact caaaccaaga tttctgtttt
     3301 gtgacgaacc caactcaggc cttgacccga agacatcaat agtcattgac gagctgatac
     3361 gggagatcac cgtagaactc aacatgacca caattgtcaa cactcatgac atgaactccg
     3421 tgatggaaaa cggagaaaag atcatattca tcgaagaggg ggagaaatgc tgggaaggca
     3481 acagaaacga gatcctgaag tcagactgca gcaaactcaa tgactttgtc ttcgccaact
     3541 cactggcgag acagcttaag gattcctcaa aataaaaacc gctctacatc gggggaagag
     3601 tcttgtccct ttccctgttg ccgaacaaca ggttgaggcc cagccgtgca ttgacggtat
     3661 tcatattctc gggaagcctg attgtctgct ccccgcttgt aaagcttgaa tacttaaggg
     3721 gtatattgtc aaccaggaca aagaactgga agggtcctcc cctcagcgcc atcccgaagc
     3781 caaggttgtc aaagcgcctg ttggccgcag tgtatgccaa tgtggttgag aagatattgc
     3841 ggaagtgaag attacctgaa agggtcactg actggtgtgc ctgtggcccc tcaaaccgtg
     3901 tctgtgacag caatcccagg gtaaagtacc tgaccggtgt gtagctgaag gctgcgacca
     3961 cttttgccgg caggtatgta gtgtactcct gcggcgaatc tgcaagctcc agaacattct
     4021 ggaggctgtc aatggtccag ttgatcagct cttcgaaatc gaggctttca tcatatacat
     4081 cctgcatggt cagaccgttg aacttgaaag tggagttgat cgtaagctgt gacaggtccc
     4141 tcttccacct gataaatccg agatcattta cggcagccga tacggccagt ttttcgctga
     4201 acctgtattc agcgcccaga tcaatgcccg cacccgggtt agccagctct ttcaggtatg
     4261 atatcgcgtt gcgggtatca tcaaaccttg cttcgtcgaa ttctgctccg tggatggtgc
     4321 cgtcactctc tgttgtgaac agtaccgggg cactcatgtt caacgagata tcagcgtcag
     4381 ccgtatgggt gaaatcttca tttaccgtaa gtttggaatg gttgtttgta aggtatgcag
     4441 acgccactcc cgacagaatt ttaagcctgc ctcctatcct cagcttttcg gtgatgtttc
     4501 ctgaagcccc gagtccgatc tcgtggtaaa ccatcatgtc tgtcctgaat gaagagaggt
     4561 caagtgtttt tccgatgaag tcactgttac cgcccagtcc cagcctgaag agatcacccg
     4621 ggaataccag gttggcttct gcccgttcag tgatgtcaag gctgaacctc aggttgtcct
     4681 taaccgtaaa ggcgagacca aaaagcctga ctccggcctg cggctcaaga gagttgttgt
     4741 cgcccagtcc tgccaggaaa tcattcagcc agggacccct ttccaggaag gaaagggtgg
     4801 agtcggagat gacaccgctt gtaaagaggt ctgacagact caggaagttg ttgtcaagtc
     4861 tgacggagat atcggatatc ccgggtagtc caaggtagaa ccggcctgtc ggcttaaagg
     4921 ccggattgag ggtagtagcc tgcggcaggt tcatgaaata aagaacctgg ctgttctgag
     4981 aatggccggc ggtgactgct gacagcagca cgatcagcgc cagaattttc cttttcatat
     5041 ggttttatta ttttgaaccg gaattcagtt cgtgattaca aaggtagatc tcatcttcga
     5101 ctgagatgag gttccaggta cttttatttg acacttttcc agtgttgaac aggggccctg
     5161 ccggacgcgg gaaaccactc acggaagctc cgtttcgtcc gatgaggtga agcatccctt
     5221 ttcctgtatc atagatggct attctcctct catccgttcc tgttgagacg atccatggcc
     5281 ctgtcacctc ccctcctccg gcactgtatc tccacatctc gtgaccggag ccgtcaatgg
     5341 ctctcagaac accctggtca acagtcaggc ggtcagtcac gtcgtcacca tcaatatcac
     5401 caaattcaga cctgtgtgag gcagagatcc ctctgatagt gtctttcttt accgtgccgt
     5461 caaacagcag cctgactgtg gcaccgtcga gagcggaaaa gataatggaa ggttcatcct
     5521 cagctgtcag cctggcaacc gaacccggag ccttcatcag aggctcctgg tgggctaccc
     5581 tgatattgcc ggtgcggtca agcacataaa ccgcctggtc atcagccacg aagagataat
     5641 ccttacccct taccctgaag aaggttacag gatcagtgac ctttcccctg gcggtaaaga
     5701 gattccatcc cctgaccggt gtgcctgacc ggtcataggc atatatcttc cggtcatcac
     5761 cggctatgaa aagcctgtag tccttgttgt tctcataatc aaagagggca agggtgtttg
     5821 aagccggcga ctgcatcttt accgggaatt tgtcaacata gttgccgttc cggtcgataa
     5881 ggtgcatata gtcacggccg gtaaaaagaa gttgcagttt accatttttg tagtaatcta
     5941 tcatgaagat atcaccggtt atcttttccc tgattgctgc cttccagagt atcttgcctg
     6001 aagaacttat aaggtaaatg ttgttgttgc ggtcctgtat gaatatttcc gttgcaccgg
     6061 tgttatggtt ggtgaagaag aacggtttaa ccgaaggtgc tgcatcaagc ttcaccttcc
     6121 acaggagttt gagcccggat gaatctgttt caccggttgt gacttctgtg gctgcggctg
     6181 cagccggagc gctccccggg tgttcaccgc cggaagtata ccttaccgag aggctcgtat
     6241 agatcatctc attgcttggc gtcatgctca ccccaacccc gctgattcct gacagcgccc
     6301 tgtcagtcag ggaggcggct gcagccgggg tgagatactc agccaactgt gacttcagca
     6361 ccctgcccga tgaataaaac agaaaggagc tcttcgtggg caggctcttt tccatctccc
     6421 tgaaacccgg gtcattgata agggtatttc cgccggctga ctccctgagc actgccgcga
     6481 gtgcttcagg agattgagag aagatcatgt atgaccttgc aaatgtcacc cattcgtcac
     6541 cggccctgct tttttccgcc ccggcaagga ttgatgccac cccggtgaat ggcatgcggt
     6601 agatcacctc ctccgctccg tcactgttcc tggctgaagc cacaaaatga ctctccctca
     6661 gtcccatgga gcggtacttt gcagccagtc gctgccgcag cacctcttcc gccgactgtg
     6721 ggtctgtcat acgaaagaga cgtatcctca cactgccctc accgaatgct atcagccctt
     6781 cagtaacctc tgtgccggtg aacgggctca gaatgagggc aaggtcagtc gcatttattg
     6841 atgccgggtc agttgccgtt tcgccggaga gtgaggcgcg gcgcatcacc gtgctgtagc
     6901 tgagggtatt gcgtggaaga agttcatgca caccgttctc agccggtgca acatccctga
     6961 ggcggtctgc acctgtacct gcacctgcag tagtcaggaa cccgcttatg aaaagcccgt
     7021 cctccgcggt ggtgagatct ccgcccccgg caattgccac tgacgagatg acggagatat
     7081 cttcagggtc agtaaatgat ttcaggaaag gcggaaggtt tctgaacagc acgaacagat
     7141 tgtcagccgt tttgccggca gcgctgacaa cctgggtgaa accctgctgg tgccgtatgt
     7201 ctgaaccggc gctcctgttg tcaagggctg cggtaatcat gctctccgag ggagttatga
     7261 tcaggatgcc ggaggtgaga gctaaataaa ctggcttctg cctgctcccc cgggtgaatg
     7321 taagggtgaa tgttcgtgtg cctccaaggt cgcgcttgtc ggtcactgtg gcccccgcct
     7381 ggctgcacag caacgtcagc ttccttgccg tgaatgcagg tccggtgctc atcactgcca
     7441 gcggcacagt ctgcctctgc ccggcagcat gaaaagaaat gagcatcctc ctgttgctta
     7501 taagctcacg aacctccctg ccgccggtga cactgtcaat tgaagaggca gagcgcacaa
     7561 gcgacgcggc ccagtccaga cctgacagtc ctgacaggat gcctgcagga tcggtaatac
     7621 gggtcagcag ctcgggaaaa tcactggttt ctatgacgaa aaaagcatcg gcaggcactg
     7681 cctcccacgg gtcaataacc accacctgct tctccttgcg gagaaagaaa gctgccacag
     7741 ccagagcaat ggcaagaatc cccaccagta tgatgccggt tctcttccgc acaaatatcc
     7801 ctgttgttat ctaacatcaa aaatataaaa tattaggatg ccccgcgaaa gagtttgacg
     7861 agacgctgct gttatgccgg gaaacatatc cgaaatgaac acgaatggaa cgctttttgt
     7921 gcgaaaagaa acgccaagac atttttacca atgaataaat tcacctttct gctgctgttt
     7981 ttagctccgt tgatgagcct tagcgcccag acagtcactg ttctcagtgc cgatgacggc
     8041 aagccgatag cagatgtggc tgtatacaac gaagccagaa cacagtttgg ctataccaat
     8101 tattcaggga aggtgagcct ctcgggattc agcggggaac agccggtctt cttccagcac
     8161 ttttcatacg agagggtttc gttcaccact gaagagctta tggctgcaaa gtgggtggtg
     8221 aagctcgaga caaaaacctt cgaggtggag gagttcatcg tctccgcgaa ccgatgggaa
     8281 cagaaatcgg aggaagtgcc caaccacatc aacgtggtgg cgctcccggc tgtaaagatc
     8341 atgaatccgc aaacggcagc tgacctgatc tccatgggcg acgtcttcgt gcagaagagc
     8401 cagctcggag gaggaagccc catgattcgt ggctttgcca ccaacagggt gcttattaac
     8461 attgacggag tgaggatgaa taatgccatc taccgtgaag gcaacattca gaatgtcatt
     8521 tcaattgacc cgaacataat agaacgaacg gaaatcatct tcggaccggg cgccaccatg
     8581 tacggcagcg atgccctcgg cggggttatg gacttccata cccggagggc tctcttctct
     8641 acgggcgact cccttttcgt caaggccgaa gcaatggctc gctggtcatc tgccgccggc
     8701 gaacagactt atcacgcatg gatcaacgcc ggcggcagga gggtggccct cctgtcgtca
     8761 gtgacctggt ctgacttcgg cgaccttgta atgggatcag tatcacactc ggaatacctt
     8821 cgcgacctct accaggagcg tatcggcaac cgtgacagcg tgattatgaa cagcaacccc
     8881 cgcagacagg tggcttccgg ctacaggcag atcaacacca ccaacaagct ccgcttcaag
     8941 gcgggaaaac acctggatat agatctggca caccattact cccacctctc cgatgtcccc
     9001 cgctatgaca ggctcatcca ggtaaagggc ggaaccctga ggtacggcga ctggtattac
     9061 gggccgcagg tgtggatgat gaccaacgcc tcgatgcgtt ataacaggcc tgcaaatctc
     9121 ttcgacgaaa tccgcctcac agtggcaagg caggattacc gcgagagccg tcatgacagg
     9181 gcgctcaaca aaactgacct caatgagcag tttgaaaagg tagggatctg gtcggccaac
     9241 ctcgacttcg acaaaaagac cggcaaaagg gataacctgg cgtactacgg agcagaatat
     9301 gtatggaaca ccatccgcag caccgccgat gtgaaaaata ttcttaccgg ggagaccgag
     9361 cctgccgggt cacgctatcc caacgggaaa aatatctata acagcttctc cctgtacggt
     9421 ggttacaagt tcaacctgag cagtaagttc acagtaagca cgggagccag gtacaactat
     9481 gtcagcctga actcggagat agctgacaac tcatggtata actttccgtt caccaccatc
     9541 gagtcaggta acggtgccct caccggtgct gccggtgccg tctacaggcc cgatggccgt
     9601 acggagctga ctgtcaacct ctccaccgga ttcagggctc cgaaccttga tgacctgggc
     9661 aaggtttttg aatcggcccc cggggtgctg gtggtgccca atcctgacct gagaccggaa
     9721 tacctctata acattgacct tggcgcatca agggagttcg gcaagatgct cctggcagag
     9781 gcgtcggtct tctggtcgta tcttgacaat gccatggtgc gcagagagtt tctcttcaac
     9841 ggagaaccca catttgtctt ccagggagag gagagccagg tctatgccat ggtcaatgcc
     9901 ggatacgcaa ttgtctacgg cacccagctg aaggccgaac tgcgaccctc atcattcatc
     9961 agggtcaaat catcactcag cctcactgcc ggacatgacg atgagaaggc tccattgagg
    10021 cacgttccgc cgctgttcgg ggcaacgcac ctgatctatg aacgctcggt tctcaaagcc
    10081 gacctgtatg cggtctacaa cggaagcata accaatgaca gaatggcccc cagtgagaag
    10141 gacaaaccat atctctatgc cgaagacgaa aacggcaacc cgtggtcacc cggatggttc
    10201 acgcttaact tcaaaacctc ctggaacatc agcaacaggc tcgaactgac agcggggata
    10261 gagaacctgc ttgacctcag gtacagaccc tactcctcag gcatcgcagc acccggaaga
    10321 aactttatcg tttccgccag aatcaaggtc tgaaatcagg cagtagccaa taaccaccag
    10381 gcaatagtaa taccccgcag gggttccgga attgtaaatg accggcacca acctgaccat
    10441 tccccgtagg ggataaagaa ttttgccctc atggcttccc tgcttctacc ttttggattt
    10501 ccaaaatgaa tgcgtaattt tcagaaattt tggtactcag agggtaatga accgtggtta
    10561 caggcttgct gaactcagca gcaaaaaaga catcagggac tttcatgatc tgccggcatt
    10621 tataaacagg gaagacaaga attggataaa gcccctcttc aaggatattg agacaatatt
    10681 tcacccttcc tccaacacgc tgttccgcac cggcgaggca gtaaggtggc tgcttcgcga
    10741 tgacactgga catgccgttg gccgggtggc ggctttctat agtaagaaaa ttgcgagcgc
    10801 ccacggagaa caggtggggg gaatgggttt cttcgaatgc attaacgaca gggaggctgc
    10861 ggcaatactg tttgacgcct gtcgcaactg gctggaggag aagggaatga aagtgatgga
    10921 gggacctgtg aacttcggtg accgcgaccg ctggtgggga ttgctggttg acggattcat
    10981 gcccccgaac tactgcatgc cctataaccc gccatactac agggaacttt tcgaggcata
    11041 cggattcagg aactatttcg accagtacac ataccacatg cctgttgacg acagcaggct
    11101 tgatgctgta atcagggaaa aggcggaaag gatcttcagc aaccctgact atacattcag
    11161 gtatatgacc ctgaaagagt tgcgtgacat ttcccgtaac tttatgatag tctataacaa
    11221 ggggtgggca cggttcccgg gtgtcaagac aatcagcgag ggccatgcaa agcttatcct
    11281 caaaagcatc aggccggtgc ttgacgagaa acttctctgg ttcggcttct acaggaatga
    11341 acccatctgc ttcttcataa tgctccccga ggtcaaccag attgtcaggc atctcaacgg
    11401 caagctcaat catgtcggaa ggttgaaatt cctgtggtac aggcatatta aaaatgaact
    11461 gaccaaggca ttcggcctga tcttcggcat tgtgccggaa catcagcgca agggggtgga
    11521 gggagctctg ataatgtcat tcgccaggtt ggcgatgtct gacaggtttc cgtacaaaga
    11581 gctggaattt aactggatag gcgacttcaa tccctcaatg atgcaccttc ttgaccagat
    11641 aggcgcaaaa ataatcaaga cgcacatcac ataccggtac ctgtttgaca gggatgcacc
    11701 cttcgagagg gccccggtgg tcaatgcgtg aatggcctgt agccaataat ctccctgacc
    11761 tcacggatgg tttttgaagc gctggccctt gccttctccg caccacttgc cacaaccttt
    11821 ccgagataat ccggatcatt gagtatctcg ttgatcctta tcctgatggg atcagtaacc
    11881 ctgatgatgt cctcggcaag ctgcttcttc aggtcgccgt accttatctg gcatgtggcg
    11941 tgctgctcac ggaaatgctg aagcgtggag gattcagaga ccacctccat tattgtaaaa
    12001 aggtttttga ccggttctga cggttcggca cccggtttct ccggcccggt atcggtaacg
    12061 gctttcatta ccttcttccg gatctctgca ggcgtatcgg ccaggaaaat gccattcccc
    12121 tcgctcttgc ccatcttacc cgtaccatca aggcccggga tctttataag ttgcctgccg
    12181 aaattatagg catcgggctc cgggaaatat tccacgccgt acattgtatt gaagcggcgg
    12241 gcaaaccggc gggtcatctc gaggtgctgc tcctggtcct ttcccaccgg caccttgtga
    12301 gccctgtgaa tgataatatc agccgccatc agcaccgggt aggtcagcag gcctgcattg
    12361 atgttgtcag ggtgcttgcg gatcttctcc ttgaatgaag cagtacgctc aagctcccct
    12421 acataggaaa tcatgttcag cagaagataa agctcggtca cttcgggcac atcgctctgc
    12481 acatagactg ttgccttctc cggatcaatt cctgccgcaa ggtattcggc aagcacctgc
    12541 ctgatgttgc catgaatgtc atcaggatgc ggatgtgtcg taagtgagtg ataatctgct
    12601 acaaagaaaa aacaattatt ttcatcctgc atcctgagga aattcttcag agcgccaaaa
    12661 taatttccca ggtgaagatt gcccgtcgac cgtatcccgc ttactactgt atccattaat
    12721 aaaatattag cagctgcaaa aatagctatt attcacgaat tcatttcatt tccggcagga
    12781 tcagtcagga gtgtcggtac cgaacttatt tcgtactttt gcggcgtaat tcatgcacaa
    12841 tggaatcgac acggcagaag aaagttgcac gtctggtgca taaggagctt gcggagatca
    12901 tcatgcatca cgggacagaa cttgcgcccg ggaagatgat atcggtgacc acggtgcgcg
    12961 tcagtcctga cctgtccctc gcaaagacat ggatcagcgt ctttccttcc gaaggatctg
    13021 ccgctaccct tgaagtttta cgtaccagcg tacccagact gaggtatgag ctgggacaga
    13081 aggtgcgcaa ccagttgcga atcgttcccg aaatcgcttt tttcattgac gacagcaacg
    13141 actatatcga caatatttcc aaactgctca aggaataagc ccgatgcact atgacccgat
    13201 caaggatctc ctggggcagg tttttaaccg ccatccgctt ctgcggaagc tcttctaccg
    13261 gctgctgaac atcctgctcc tccgttcatg gcacgtcagg agggcactga aaaacctggg
    13321 gccttcgctt ccggacagcg caaatgtcct tgatgccggg atgggcttcg gccagtactc
    13381 gtggtggatg gcctcgcatt tcacttcgtg gcagatcacc gcggctgata ttaagagcga
    13441 acaggtggca gactgcaacg aattcttccg ccggaccaaa cttgcaacaa gggtgcacgc
    13501 cgttgaggct gaccttgtta cctggagcag cgtggagagt tatgacctga tcctttgcgt
    13561 tgacgtaatg gaacatattg ctgaagaccg cactgttttc gccaatttcc tgaagatgac
    13621 tgccgggggc ggttacctgg tcatctcgac cccctcggac cagggcggtt cggatgtcca
    13681 ttccgacggc gacacgtcgt tcatcggtga acatgtgcgc gacggatatg ccagggagga
    13741 gataacggag aagctccgga ccgcaggata cacagaggtc agcgtgtcat atacctacgg
    13801 caccccgggc agcattgcat ggaggctttc gatgaaatat ccggtcatca tgctctcagc
    13861 atcaaaactc ttctttattc tgctgccttt ctactatctt gccgttatgc cgttcgtgct
    13921 ggcgctcaac tgcgcagacc tgaacgggag ccaccggaag ggaaccgggt tgctggtaat
    13981 tgcccgcagg agcagatgaa gctgtcaatc tacatagcaa aaagatacct cttcgcaaaa
    14041 aaatcgcgga acgccatcaa cgtgatctca gccatctcag tggcaggggt gacagtcgga
    14101 acaatggcgc tgataaccat cctctcggtc tttaacggcc tggaagatat ggtcagaagc
    14161 atcttcagca cctctgaccc ggagataaaa atcacccccg ttgccgggaa ggtgttcacc
    14221 cccgacagtc tcatgctgaa aaagctggct tccgccgaag gtgttgacgt ctatgcgatg
    14281 acactggagg aaaatgctct cctccgctat gatgagcagc aatatatagc taccgtgaag
    14341 ggagtgtcac tcaactatgc cgctgtcact gacctcgata ccgcaatgtg ggacggtaac
    14401 ttcatcctgc agggcgaaaa cgggaatccc tatgcagtag ccggactggg tgtggcaaac
    14461 tacctcggaa tgcggcttaa ctttatctcc cctctggcaa tttatattcc tgaccggaag
    14521 gcaaaaatca ggggaacgcc tgacaatgag ttcacaagga agtatatttt cctctcggga
    14581 atctttgctg tcgaacagga attcgattca aaatatgtct tcctccccct tgactttacc
    14641 agggaactgc tcgactacac cgacgaggta agttccatag aggtgaggat gaaacccgga
    14701 gctgatgaga aaaagacgca gaatgcaatc agggaggcaa tgggtgacgg tttccgcgtg
    14761 caaaaccgct atgagcagca ggagatattt tacaaggtga tgaaggctga gaggctggct
    14821 atcttcgtta tcctctcatt catcctgatc atcgcttctt tcaatatcat cggatcactg
    14881 acgatgctga ttattgaaaa agagagggat ataagcatcc tgaaaagcct gggggcagat
    14941 aaccgcctga tcaaacggat attcatctat gagggctgga tgatatcctt tatcggaacc
    15001 atcattggcc tcctgctggg attcgtcctc tgtgcggcac agcagcactt cggcatcgtc
    15061 aagctggcag gtgagtcgtt gcttattgac gcctatcccg tcaggatgcg cctggctgac
    15121 ttcttcgtgg ttgctgcaac ggtgcttgca ataggttacg cagcagcatg gtaccctgtt
    15181 cattatctct cgcgcaggca acttaaagac aaggaaaaag ccattaaatg aagacggtat
    15241 acaaaatatt cgttgtgact gtcatgctga tgattatgtc atgcggcgac tcggaaaatg
    15301 aaaaaccgtc gcggaagatg cgcatcccgg aagataagct tgtcaggata ctcactgaca
    15361 cctatctgac ggcaggtatg atggatctca tttccatgag ggagacctgg gctcagcgtg
    15421 actcaattca gaactatgca gatgtcatag agagccacgg ttatacctat gaacagtttg
    15481 aaaccacgat gaggtactat ttcacgggta aacccaggaa gctgtcaagg atatatgaca
    15541 gggtaaccgg gaacctgctt gaactggaaa cggaagtgat gacccaacag aatgcggtga
    15601 agccttctga aaaaaacctc tggacaggca agcccgacta ccgcctgccg gaagacttca
    15661 cccgtgatcc tgtctggttt gacatcccgg ttgacaggcc cggtgaatat atccttaagg
    15721 ccgacatccg tgtttttgaa gacgataaga gtcttaatcc gagggtcacc gtttacttca
    15781 gctaccctga cagcaccgga gaggaaaagc gcgactactg gcaggaaatc ttccttggaa
    15841 aggacgggca gatccgtaac gttcagatca gccataccct tgacagtatt cccggctcac
    15901 gcctgcgcgg atggctcctg aatcatgaca accagcctgg taagtgggaa aagcatgccc
    15961 gcatagcgaa tatcagaata gtgcacgctg acgacttcgc agtacagtga cagtgagaag
    16021 aatatctgcc caatacctgt ttacctcggc cggaaggcca ctcaggagag gcatcgtcac
    16081 cgttgccggc gacggcaccg tgaccggtgt cgaggatacc ggcggctctc tcagggagag
    16141 tgcttcaacg gagttttaca acggcgttat catcccggga tttgtcaact gccattcgca
    16201 tcttgagctt tcccatatgc gcggccttat tcccgccgga ggcggactga gtgacttcat
    16261 tgtctccgtt cgcgacaggc gcgaggcatc agatgatgaa atcatggctg ctgtccgggc
    16321 cgccgacatt gaaatgcgtt cgggcggggt ggtggcttgc ggcgatatct caaacagccc
    16381 ggtcactgcg gtcacaaaaa gcaaaagccc catccgctac atcaccttcg ccgaggtctt
    16441 cggcctggac ccggctgttg cggaagtccg catgcgggga gcccttgagg tgtgtgagga
    16501 gatgaggcgc cggagtcttc aggcacaggt gacacctcac tcactctatt ccgtctccgc
    16561 tcccctttct gaaatgataa gggaacagat ttcacctgat tcagtcatct cacttcattt
    16621 cctggaatcg gatgacgagc gcaaaatggc acagggccac gcagatgcag ctcttggcct
    16681 ctcccgtctt gcatcacgct tgttgctggt acacaacacg gtgatcacca gggaggaggc
    16741 actgaaggtg gcatctgcag ggaacacctg gttctgcctc tgcccatcgt caaacatgta
    16801 tatatccggt aagatgccac ccgctgcgat gctgcgggaa ttgaccggta agattgcagt
    16861 gggaactgac agccttgcat cgacatgtcg gctgagcatg atcagcgagt tgctgctcct
    16921 tcaggaggcc gtgccgggca tccccgcgga ggaactgata aggtggggga cgatcaacgg
    16981 ggccatggca ctgaagtgtg atgacatact cggatcaata gaacccggca agcgccccgg
    17041 cctgctgttg gtggagccgt tcgacctgca gaatatgcgc ctgcttccgg agagccgggt
    17101 gcggagactg gtataacata tgtatctgct ccctgagagc cgcgtgtgga gactcgtata
    17161 gcacttgcgt ctgcttccgg agagccgggt tcggagattg gcatagtccc ccccgcagtc
    17221 gccggcatga acctggccct ggcaggcata tacttcaccg cagagccgga tatgcatatt
    17281 atcgtaaatt tgtagccttg atcaatcatt cgcgtaatgt ccactttcct ttttgacagc
    17341 attgtgttcg ggccggtgca cagccgccgg ctgggcgtat ccctcgggat aaacctcctt
    17401 cccgtcaccc gcaaggtgtg ctcctttaat tgtatatact gcgagtgcgg ctggacaccg
    17461 ggcaaaccgg ggcctaagga aaagcttccg acacgcagcg aagtccgcga tgcccttaga
    17521 aagaaacttt ccgggatgaa ggagcagggc aaggccccgg atgttatcac ctatgccgga
    17581 aacggcgaac ctacactgca ccccgacttc gccgtgataa tagatgactc cctggcactg
    17641 cgcgatgagt tcttccccgc ggcaaaggtg tcagtgctct ccaacggttc catgctccgc
    17701 ctgaaaagcg ttcgtgaagc gctgaagaaa gttgaccaga acatgctcaa acttgattcg
    17761 gcactgccgg atactgtcag gcagattaac cagccgcatg tgaaaactga ccttgccagg
    17821 tatgaagagt atatcaggga gttcaagggc aggtttatca tccagaccct cttcgtcagg
    17881 ggtgagtgcg acggccggac ggttgacaac accactgagg aagagatcac cgcatggctg
    17941 gaagccatat ccaggctcag acctcagcag gtgatgatat acaccataca ccgcgatacg
    18001 ccgctgggca acagtctcag aaaagtgccg gtaagcgaac tcaatgccat cgcccgcagg
    18061 gtcagccagt tgggtatcac cacaaccgtc tcgggataac agccatgaac gggatgaaag
    18121 tcctgataag ccctctcagc tggggtttcg gccatgccgg aaggatgata ccccttgcga
    18181 tggagttgga acgaaggggc tgtgaggtgg tcttcgcagc tgatgcaccc ctgaccgaaa
    18241 tgatcgtaag ggagctgccg ggtatcaaaa cggttgaaat tccgggcctc aggatcaggt
    18301 actcccgtta cctgccacag tatgtcagca tctttctgca attgcctgtc atagttgcgt
    18361 ccgcggtaag ggaacatgcc accctccgca ggctggcgaa agaacttgac ccgatggtga
    18421 tcatctctga taaccggttc ggcttctgcc ataagaaaat cttttcagtc tatgtcaccc
    18481 accaggtgag gatcgctttc cccgcgttcc tcgcgttcct ggagccgctg gctgcgtgga
    18541 tgcaccggat gataatcagc aggtatgatc tctgcctggt gcctgattat cgcggcagcg
    18601 aaaatctttc gggaaggctc tcgcatggcg gcaggcttcc tgcgaatgtt gcctttatcg
    18661 ggcccctctc aaggtttgga gttgccggta ctgaaccgtt tccgccctgc cggatgaaga
    18721 gtggaccatt atcgccctgc cgtacgaaga gtgaaccatt gtcgccctgc cgtacgaaga
    18781 gtgaaccatt gtcgctttcc cgggcgaaga gtgaaccttt gcagccggac acgggggcgc
    18841 ctccctcatc cctggctcag ccttatgcct gccttattct gtcaggacct gagccacagc
    18901 gatccctgct gctccggaag gtttctgaat ctctgcggga attcccgctg gcagtgctga
    18961 gtgccacacc tctgaataca cctctgcctt ccggccaggc aatacattta ttaaccggag
    19021 ctgaaacagg cacaatgcgg caggttatta ccggggcatc catggtgatt gcaagaaccg
    19081 gatatacttc agttatggag cttgtctccc ttggcatggg agcagtgctt atacccaccc
    19141 cgggacagac ggaacaggag taccttggca ggcaccttga cggacgttat ggttttatca
    19201 ccatgaatca aaacaggctt gaaaggctgg cttcagtgat cctacctgag cataaaaaag
    19261 agtgacaggc ccattatcac tgtcccggcg gccagtcctg cccagacctc cgagggcgaa
    19321 tgatcaccga ggtatattct tgatgacatc actgctccgg ctgttagcgc aacggctaca
    19381 gcctgccaga gcatgctgat gttgtacact gccgccatga agataaccag caccatcaat
    19441 cccccgatgc ctgaagcatg cagcgagatc ctgtaaaaca ttgttatcag aagtgtgctg
    19501 aatgtgacaa tagctattga gatgaaaaaa gccctgaaga gacctggaac ctgcatcctt
    19561 agcagaatga aggctgtcag gctgtacatc agaagtgaaa atgaaagcag gattaccctc
    19621 tcattgcggt cgcgggcatt gaatgttttg atggcgcccc gggcgtaaag gacagttgcg
    19681 agggccattg gcataagcag gttattggcc atcacaagta ttaaaatcat ccttttgatc
    19741 tgggggggaa gaaatgagtg cagcgtgtgt gcgctgtaaa taatcagcag tccgtacagc
    19801 ggcaggaata tcgggtgaaa aacaactgaa agtatttcag ccgctctctt cagccactgt
    19861 tcattgcccg gatgcatcct gctctcctcc tcacctctgc cattgccagg cagtccattg
    19921 tctccggggt tcattttatc tccttcctaa tcctgaaaat ctcaaccagc cagaatgctc
    19981 tgctgcctat tttatctcct tcctcatcct tgcaacagga atattcagct gttccctgta
    20041 cttcgccacg gtgcggcggg ctatctggta gcccttctcc tgtagaattt ctgtcagctt
    20101 ctcgtccgtc agcggcttgc gcttatcctc attgctgagg cactcctgca gtatcctctt
    20161 tatctcgcgg gtcgatacct catcaccggc atcagtctgc atcccctcgg agaagaagaa
    20221 cttcagcggg tagatgccga aatgggtctg tatgtacttg ctgttcgcca cgcgtgacac
    20281 cgttgaaatg tcaagccctg tcatctcggc cacatccttg agtatcatgg gcttgagttt
    20341 ggtgtcatcg ccttcggtga aataatcctt ctggtactcg aggattgcgt tcatcgtcag
    20401 cagcagggta ttctgtctct gtctcatcgc atctatgaac cacctggcag catcgagctt
    20461 ctgtttcacg aacatgatgg cgtcacggtt ctgcttgcca gccttcttgt cacgggagta
    20521 tgtctcaagc atctcactgt agtcccggtt gatcttcagt tcggggatgc tcctgttgtt
    20581 caggctgagc tcaaggccgc tgtcactctg ttcaaggatg aagtcaggaa ttatatgctg
    20641 tgccgatctg gcatacgggt cgcttatggt gccgccgggc ttgggattga gtttcaggac
    20701 ttcgtcaatg atccctttca tctcgctctc gccgatcccc atcctgttca tgatcttttc
    20761 atagtgccgc ctggaaaact cttcgaagta atctgtaaga acagttttgg caagagtcac
    20821 ggaggggaca gactggtctt tcctccctat ctgaagcagc aggcattcgc gaaggtcacg
    20881 ggcaccaacg ccagccggtt ccagatcctg tattatcctg agtatcgcat tcagctcgtt
    20941 gacatctgtg gtgatgttct gcaggaatgc aaggtcatca accatgttct ccacctcgcg
    21001 gcggaggtag ccgtcgtcgt ctatgttgcc gatgatatac tcaccaagga ggtcctcccg
    21061 ctcggtgtct gtgcgaaggt tgaactgctg tatcaggttt tcgtggaatg atgatccggc
    21121 ggcatatgga atatcttcgc gccgttcatc atctttcgat gcgttgttga cctgaagcct
    21181 gtaatcaggg atatcctcgt cattcaggta atcatcgagg gcaaactcat cctgttcgcg
    21241 ttctgcctgt tcgtcatcaa cctcatcgtc atcctcatcg ccctgtacct catcctcttc
    21301 aggcccctct tccagggcgg gattctcctc cagctccttc ttgatacgct cctccatctg
    21361 catggcaggc acctccagca gcttgatcat ctggatctgc tgcggggaga gcttctgtag
    21421 cagtttctgc tggagtttct ggtcgagcat ggagtctatt gttcagcagt ttagaattca
    21481 gcgttcctgg gagtacgcgg gaacggtacc acatcgcgga tgttagacat accggtcatg
    21541 aaaagaatca gccgcgcaaa gccaagtcca aagcccgaat gcggtgcggt gccaaacttt
    21601 ctggtatcaa ggtaccacca cagatccttc agcggcaggt tcagctcctc aatccttcgc
    21661 gtcagcttat ccagctcctc ctccctctgc gaaccgccta ttatctcgcc gatgccgggg
    21721 aagagcacat ccatggccct gacagttttg ccgtcatcat tctgcttcat gtaaaaagcc
    21781 ttgatgttca tcgggtaatc ggtaagtatc accggtctcc tgaagtgtgt ctccacgaga
    21841 taacgctcat gttctgcctg caggtcacg
//
LOCUS       DAOPXU010000043        21600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_2477_length_21600_cov_11.150940, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000043 DAOPXU010000000
VERSION     DAOPXU010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21600
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2477_length_21600_cov_11.150940"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..559
                     /locus_tag="PKK03_06025"
     CDS             44..559
                     /locus_tag="PKK03_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX83992.1"
                     /translation="MERSEKKLAELGRILEKGTGREINDRIRLLRTEVPFGGALKMLA
                     LFYDKTGDHNLRAAISGLFNDMKEKSAMAEVIDSVSAVRKPDSRVMLISSCWQSGLDY
                     SPFAVPLAEHFVSGDYMTSLECFTVLDTCAASISHNDRARIIITLQEKKESWETPKQK
                     LAGELITLLKG"
     gene            complement(587..1564)
                     /locus_tag="PKK03_06030"
     CDS             complement(587..1564)
                     /locus_tag="PKK03_06030"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNX83993.1"
                     /translation="MSTINTRYLGLDLSSPIIVGSSNFTSKVESIVEAEAAGAGAVVL
                     KSLFEEQIVSQAHSMSSRESYPEADDYLQYYTRTNSVDAYLDLIAGARKKVKIPVIAS
                     INCFSPKGWIDFALEIERAGASALEVNIFFIPTDKHENSTDSEKVYFRIIDNLKKKLK
                     IPVSVKIGQKFSNILNFVWQMYNHGAAGVTMFNRFYEPDFDINNLNIVPASVLSNPAE
                     QRYVLRWVGMVSAQDIKIDISASTGVYTGEDAIKYLLAGATTVQVCSALYKRGFTVVS
                     EINETLARWMNNKEFRSISDFRGKLNYRNVDNPSLFERSQFMKHYSSYI"
     gene            complement(1561..2244)
                     /locus_tag="PKK03_06035"
     CDS             complement(1561..2244)
                     /locus_tag="PKK03_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNX83994.1"
                     /translation="MNNIIAENIRKLLDELPSGISVVAVTKTRTPEEALEAYRAGLRI
                     FGENRVQEMMTKRPLLPDDISWHLIGHLQSNKVKQAVAAASVIESVDSVRLLRLIDAE
                     ALAQGKCTDCLLQVHIATEETKSGFSLEEMAETDWRHVSDSLKAARICGLMGMATFTD
                     DREKVRSEFRSLSDLFSRMRKQYFTGKSHFRELSMGMSGDWRLAVGEGSTMIRVGTLI
                     FGERIFKAR"
     gene            complement(2260..3510)
                     /locus_tag="PKK03_06040"
     CDS             complement(2260..3510)
                     /locus_tag="PKK03_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNX83995.1"
                     /translation="MAVVKPFRGLRPPANLASEIACLPYDVMNSEEAAVMARGKEKSL
                     LHITKAEIDLPDGTDVHSEEVYQKALENFISWQKKGWLIGDLERHFYIYAQTMKGRTQ
                     YGIVGAAAVTDYLSGIIKKHELTRPDKEEDRMVHVRANNANIEPVFFAYPSVRELDKI
                     VEKIVKNERPEYDFVAEDGIGHHFWVVWDRKTNDKIEKLFAEKVPYMYVADGHHRTAA
                     AALVGKEKKDKNPAHRGDEEYNFFMAVIFPDDQLKIFDYNRVVKDLNGMTPAQFLGKL
                     KESFDVREKGTRTHRPTKLHNFGMYLDGKWYSLTAKKGMYYDDDPIGVLDVTILTELV
                     LLPLLDIQDLRRSKRIDFIGGIRGLGELKKRVDSGEMKVAFALYPVSMKQLITIADTG
                     NIMPPKTTWFEPKLRSGLVIHKLD"
     gene            complement(3528..4454)
                     /locus_tag="PKK03_06045"
     CDS             complement(3528..4454)
                     /locus_tag="PKK03_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX83996.1"
                     /translation="MKILVATDKPFAPVAVTMIREVTEKAGHTLALLEKYKTPQELHA
                     AVADADAMIVRSDIIDAAVIAAAPKLKIVVRAGAGYDNLDLNACTAANVVAMNTPGQN
                     SNAVAELAFGMMVYGARGGFDGKSGSELMGKTLGLHAFGAVGRCMARIASGFGMKVCA
                     FDPFVDSKVIEEAGVTPLATMEALYSGCQYVSLHIPANEKTKGSIGERLLSLMPQGAV
                     LVNTARKEVINEAELVAVMEKRSDLKYLSDVAPDNAELFAQKFAGRYFFTPKKMGAQT
                     EEANINAGVAAARQIVSFFATGDQTFRVNKPK"
     gene            complement(4472..5542)
                     /gene="serC"
                     /locus_tag="PKK03_06050"
     CDS             complement(4472..5542)
                     /gene="serC"
                     /locus_tag="PKK03_06050"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNX83997.1"
                     /translation="MKKHNFYAGPSIMPQYTNEKVIEAIRDFAGTGLSIMEVSHRSKE
                     FEAVMESAVSHIRELLDIPAGYSVIFLGGGASLQFAMLPMNMLEKKAAYINTGEWAGK
                     ALKEARLFGEVVEVASSKDRNFNYIPRNFDIPSDVDYFHITTNNTIFGTQWKKDPDVK
                     VPLVADMSSDIFSRPVDVSKYSIIYGGAQKNLAPAGVTFVIIKDSALGKVTRPIPTML
                     NYKTHIEKGSMFNTPPVLPVFAALKTLEWLKQKGGLAAMAKINQEKAALLYDEIERNK
                     LFQVTVPDPEDRSLMNVCFVMSDSYKELEKEFGDFAKSKGMVGIAGHRSVGGFRASLY
                     NALPIESVRALVECMKEFANKY"
     gene            complement(5624..6340)
                     /locus_tag="PKK03_06055"
     CDS             complement(5624..6340)
                     /locus_tag="PKK03_06055"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNX83998.1"
                     /translation="MTQNSLEICFTPAAFDAHADSEAVVVIIDVLRATSSICTAFANG
                     VTEIIPVETVEEARAMKARGHLVAAERDGYVLDFADFGNSPFNFTPEKVKGRSVVYST
                     TNGTGIMKRASVCHDVVIGSWLNMSALCDWVAEQGRKVVIVCAGWKKRFSLEDAVCAG
                     ALAARLLESGKFCTVCDSVHAATDLWSMAKTDLLGYMEKAAQRSRLRDKGLDDCIPYC
                     HTFDVTEVIPVLRGDRLTAL"
     gene            complement(6365..7474)
                     /gene="gcvT"
                     /locus_tag="PKK03_06060"
     CDS             complement(6365..7474)
                     /gene="gcvT"
                     /locus_tag="PKK03_06060"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNX83999.1"
                     /translation="MKNTPFLKYHEALGARIIPFAGYNMPVEYAGITEEHLTVRSGVG
                     VFDVSHMGEFWVNGPRALDFLQHITSNDVSALTDGKVQYTCFPNGRGGIVDDLLVYRF
                     SAEKYLLVVNAANIEKDWEWCVKHAAAFGITAGPGGELNNASDGTAQLAVQGPLALKA
                     MQKLTGEPVTDMEYYTFKVLKFAGIDDVIFSTTGYTGSGGCEIYIRNADAPRLWEEVF
                     RAGEEYGIKPIGLGARDTLRLEMGFCLYGNDINDTTTPIEAGLGWITKFVPGKDFIDR
                     DLLLKQKEEGTDRRLKGFVMIDKGIPRQHYEVVDAQGNLIGEVTSGTMAPSLKQGIGL
                     AYLNKGFWKNDTEIFIRVRGRDLKAKVTALPFYKQ"
     gene            7626..7979
                     /locus_tag="PKK03_06065"
     CDS             7626..7979
                     /locus_tag="PKK03_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNX84000.1"
                     /translation="MEYRIFKPEYLLSITGGDMETMSEIAGIFGSQVPEFLTGMKSLL
                     GQEKYYELGLLAHKAKGSVTVLGMDETAKMLKEFELLAKAGDQKEKYTGFISRFEADT
                     STVMTEVEDYLTKNR"
     gene            7986..>8224
                     /locus_tag="PKK03_06070"
     CDS             7986..>8224
                     /locus_tag="PKK03_06070"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNX84001.1"
                     /translation="MVVTDRTDGIEILSFRTDRINALNVDVIRPVILKLFETPYTKVV
                     IDMEGVSYLDSTAFAMFLYLLRVARSNYCTYRLCSL"
     assembly_gap    8225..8324
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8325..10753)
                     /locus_tag="PKK03_06075"
     CDS             complement(<8325..10753)
                     /locus_tag="PKK03_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX84002.1"
                     /translation="MNFENFTIKAQESVQKAVTIASGKGQQAIECGHLLKGIMTEAES
                     IVSYIMSKTGGNSAAFGKAVDAIIDGYSRVSGGEPYFSPDAAGVFRRAADHAARMQDR
                     FVSVEHLLLALSESTDSVSRLMKDNGMDTAALSMAIADLRKGAKVSSQTSEETYDSLS
                     RYAIDLNERARAGKLDPVIGRDEEVRRVLQILSRRTKNNPILVGEPGVGKTAIAEGIA
                     QRIVRGDVPEDLMQKRIFSLDMGALIAGAKYKGEFEERLKSVVNEVIASDGDVILFID
                     EIHTLVGAGKGEGAMDAANILKPALARGELRAIGATTLNEYQKYFETDKALERRFQVV
                     MIEEPDRADAISILRGLRERYETHHKVIIRDEAIISAVDLSYRYITDRFLPDKAIDLI
                     DEAAARLRLEMNSVPEEIDTADRQIRQLEIEREAVKRDGDAVKEKELSKEIADLAAGR
                     DELKARWRAEKDLVENLQKKKQAGEELRFEAERAERAGDYGRVAEIRYGKLTDNEKEI
                     EKLNTEIAEKRNAGSMVRDDVRSDDIAEIVSRWTGIPVSRMLESEKEKLLRLEEELHH
                     RVVGQDEAIRAVSDAVRRSRAGLQDEKRPVGSFIFLGTTGVGKTELARALAEYLFNDE
                     NLMTRIDMTEYQERHSVSRLIGAPPGYIGYDEGGQLTEAIRHKPYSVVLLDEIEKAHP
                     DVFNILLQVLDDGRLTDNKGRTVNFRNTIIIMTSNLGSSLIRESIEESSGAMTEEKAE
                     ELRADLVQMLRTTLRPEFVNRVDEIVMFRPLTLMQIKEIVMMQFSRVAQMLEEKGISS
                     ELTEKAVAWLA"
     gene            10910..13984
                     /locus_tag="PKK03_06080"
     CDS             10910..13984
                     /locus_tag="PKK03_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX84003.1"
                     /translation="MNEKILNTLMHLFAIIAPAQGNENDRRGVVEAFLRPQLNQEGVK
                     AYLKIFEAYYAEAQERLKKGKEARRNSSISVRIIKICFDIGMQLTLNQKIIVLVQLLE
                     YCRSDHKGVSNTELEFIITAAEGFNINPEDYQLIQQFVLSSKDEIPESPQILVIDSDR
                     VTQYEKAHHIVNDSFRGQVRVLNLPQADMLFIRSFGTGELLLSGQLRHEDRVYLFERG
                     ASLKFMSSKPIYYSEVIRQFLDESALASRVVYEVNEIEYKFKGGQVGLHRMSFAEESG
                     RLIGIMGASGAGKSTLLSVLNGTNRPDSGEVLINGINIHNDPDKIKGLIGFVSQDDLL
                     IEELTVFDNLFYNAKLCFGNLDDEAVTHKVDDVLHSLGLYDIKDMKVGTPLNKKISGG
                     QRKRLNISLELIREPAIMFLDEPTSGLSSLDSENILDLLNDLKLKGKLIFVVIHQPSS
                     DIFKMFDRLIFLDSGGYMVYYGIPVRAIDYFKDRVHLPRYNDSECHACGNVNPEQIFS
                     IVESKVLDEFGRPTMTRKVSPAEWSSYFNSRREERPPHPRSGNGIPEITLRTPGRLKQ
                     LAVFVKRDILAKLSDSQYLIITLLEAPVLALFLAFIIRYFDESVSKPAYTLIQNDNLP
                     VYIFMSVIVAIFMGLTVSAEEIIKDRKILKREAFLNLSWNSYLLSKIGVQFLISAIQA
                     ASFVAVGNSIFGIRGMWFEYWLVLFSCWATSNMMGLLISDSFKTVVTIYILIPFLVIP
                     QIILSGIIVKYDKLNPTISSPVSIPVYGEIIAARWGYEALAVDQFINNRFEKQFYGYD
                     KAISTAKFRKDIWYNEMKSIISRLESNLERERLDESSGNDMMLIRNEIEEELGFTTAV
                     PFDLRILNPDAITAEALAAARDYIERVRRYYIEVSRNAVEARDRAIMAYEQADREAFI
                     LMKKQHTNESLEEFVRNDNEKDKIKRYRDRLYQNYDQIFYDPAHPLVKAHFYAPQKQV
                     FGLFAGTLAVNTAVIWFMTLCLYILLYFRVLHRILDSSEKFIRVMRAKRERG"
     gene            complement(14070..14450)
                     /locus_tag="PKK03_06085"
     CDS             complement(14070..14450)
                     /locus_tag="PKK03_06085"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HNX84004.1"
                     /translation="MEPIIIEGTPKTPSIKFDTQDGVFEIKGRSIPENSVEFYKPLNE
                     WLDQYMQTPLDKTVVNIRLEYFNTSSSKCILDVFKRLEAIHRSKHDVEINWFYEEDDE
                     DMLEAGEDYDSIIKVPFKMIEIVE"
     gene            complement(14476..15030)
                     /locus_tag="PKK03_06090"
     CDS             complement(14476..15030)
                     /locus_tag="PKK03_06090"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HNX84005.1"
                     /translation="MSFDLENLVSDLDIRGAVLYYKGNVDSDTITKALDLVEKKLFAT
                     REAPRLRKKVYNVLVESLQNLYHHSDKVPRGYHAARPDRFGLVVMEKIGRGYRITTCN
                     FVAAVRVKELEEKLTRINRSTPEEIKEQYKDILNHQEITEKGLGGLGLLDIARKTGHR
                     IDFRFKKYDNLNTAFRLTAVVAQV"
     gene            complement(15172..16323)
                     /locus_tag="PKK03_06095"
     CDS             complement(15172..16323)
                     /locus_tag="PKK03_06095"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNX84006.1"
                     /translation="MKLLAKTHYGLEQVLAEELKSLGAAGVTVLNRAVAFEGSKLLLY
                     TVNYRSRLALSVLMPVSSFGISGQKDLYNGAVAVEWDRYLDPGKTFAVTAVVNSPHFP
                     HTGYAALVVKDAIADFFRRLTLGRPSVDAKDPDLLVNVHISNNRVTISLDSTVVPLYR
                     RGYRQGMSEAPLSEVLAAGLIALSGWKPGTPLTDGMCGSGTIVTEAGFISCNIAPGKM
                     RKSFGFMRWKDYDAALYRSVRFDAEKREIKSPAAIFGSDISADTCAIARANVRAAGLE
                     DTVEITERDFFASPPPARSGTLIINPPYGRRLGTPDMDEFYGRIGSVLKHSYEGYTAW
                     ILSGDMVSLKKVALKPFRKFDLLNGDIECRFQGYELYGGTRKNRDVSGA"
     gene            complement(16323..17927)
                     /locus_tag="PKK03_06100"
     CDS             complement(16323..17927)
                     /locus_tag="PKK03_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX84007.1"
                     /translation="MTKKLIVTVIAALLMQCTSLRSQGLTQETFKLGKTLALLEAIYV
                     DSVNISALTEQMIISTLRNLDPHSVYIPAKDVQEENEPLQGNFEGIGIQFNLLNDTII
                     IISPIPGGPSEKVGIMAGDRIISIAGEKVTGVGMTTTGVQKRLRGPKGTKVDVLIFRK
                     GEKELLDFMITRDKIPVNSLDAAYMVTGNVGYIKLSRFSEQTAVEFSQAVEKLRTGNM
                     EHLVIDLRGNSGGYMVPAVQIADELLSGEELIVYLEGLHTSRQEYQSAPSGELTRARV
                     AVLLDEGSASASEILAGAVQDWDRGVIIGRRSFGKGLVQNRFPLPDGSEIRLTIARYY
                     TPSGRSIQTPYDEGFDKYFQTFYSRYFSGELIHPDSLNLPDSLKAFTIRNKRVVYGGG
                     GISPDIFIPIDTTYYSDYYRDLIRTSALTNYMLSYTDRNRSQLTRRYRSFDAFSREYE
                     IDAAAIEGLKAAGEKNGVRFDQKQYDVSAPEIKSVMKALVARDIWDMNEYFRVINEND
                     PAISKALEVLNDSRFYETLLGYRKPQ"
     gene            complement(18039..19568)
                     /gene="guaA"
                     /locus_tag="PKK03_06105"
     CDS             complement(18039..19568)
                     /gene="guaA"
                     /locus_tag="PKK03_06105"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNX84008.1"
                     /translation="MQEKILILDFGSQYTQLIARRVRELNVYCEIHPFNHFPLPDSSV
                     KGVILSGSPWSVRDARAPFPDLKGIKGRLPLLGVCYGAQYLTHGYGGEVMASDTREYG
                     RAKLVKVDTSDPLFDGISPGTQVWMSHGDTIMRVPEGYIITGSTEDVRVAAFRIAGEN
                     TWGIQFHPEVYHTTEGTAMLRNFVRVICACNADWTPGSFVEMTVNSLREQLGDDRVVL
                     GLSGGVDSSVAALLLDRAIGDRLTSIFVDNGLLRKDEYESVLDTYRRMGLNVRGVDAS
                     AEFVEGLRGVDDPETKRKIIGRIFIEVFDREAHKVQNVKWLAQGTIYPDVIESVSVNG
                     PSATIKSHHNVGGLPEKMHLKVVEPLRLLFKDEVRRVGNAMGMPAEILGRHPFPGPGL
                     AIRIVGEVTPSKLETLRDADEIFISGLKKNGFYDKVWQAAVILLPVQSVGVMGDERTY
                     ENAVVLRAVTSTDGMTADWSHLPHEFLAEVSNQIINRVKGVNRVVYDISSKPPSTIEW
                     E"
     gene            complement(19631..20545)
                     /locus_tag="PKK03_06110"
     CDS             complement(19631..20545)
                     /locus_tag="PKK03_06110"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNX84009.1"
                     /translation="MAFRLTILGTSSALPTSDRYPTAHVLNVHERLFLIDCGEGTQMQ
                     MRRYRIRFGRINHIFISHLHGDHFFGIYPLLSTYNLMGRKIPLNLYAPAPAEELITRH
                     LADFDITLGFDLAVHTVTGRTLKKVLDDKRVEVFSFPLKHRIPTYGYLFREKMPDRNM
                     IKEKITEYGLSIEEIATARRGGDITRENGEIIPCAGVTLEPRRPASYAFCSDTGWFKK
                     LPSYVKGVDLLYHEATFGADNNELAVKTRHSTASHAAKVALEAGAGRLLLGHFSARYR
                     STALLEDEARTIFPDTEAAREGNTYEIG"
     gene            complement(20560..20898)
                     /locus_tag="PKK03_06115"
     CDS             complement(20560..20898)
                     /locus_tag="PKK03_06115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNX84010.1"
                     /translation="MDFNIDNKKQCTVISIETEKLDTHIAPSLKSELVLISGKGERNI
                     ILDLKKCQYCDSSGLSAILVANRLCKNAGGTFVLCGLNEAVERLITISQLDTVLSITA
                     DTEEAEKLIS"
     gene            complement(20929..>21600)
                     /locus_tag="PKK03_06120"
     CDS             complement(20929..>21600)
                     /locus_tag="PKK03_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNX84011.1"
                     /translation="EQKYSINSRHKARVRNFTNFGVFVEIEEGVDGLIHISDLSWTKK
                     IKHPAEFTAIDAEIDVVVLEIDKENRRLSLGHKQLEENPWDVFEDLFTVDSIHEGTVI
                     EVFDKGAIIALPYGVEGFATPKHLVKEDGTQAAVEDKLNFKVIEFNKGAKKIIVSHSR
                     VFEDERKGSETASRRSEADNTKKAARKIKASLEKTTLGDITELQALKDEMEEKEAKSL
                     GKKKK"
BASE COUNT         4994 a         5874 c         5123 g         5509 t
ORIGIN      
        1 tgaacttatt atttagccat aaatttgttc caaagcaaac cggatggaaa gatcagaaaa
       61 gaaactggcc gaactgggaa ggatactcga aaagggcacc ggaagagaga ttaatgaccg
      121 tatcaggctg ctccgcaccg aagtgccttt cgggggtgcg ctgaagatgc ttgcactgtt
      181 ttatgacaag accggcgacc ataacctcag ggccgcaata tcaggcctgt tcaatgacat
      241 gaaggagaaa tcggccatgg ccgaagtgat tgactcggtc tcggcagtta gaaaaccgga
      301 ttccagggta atgctcatct catcatgctg gcagtcagga ctcgattatt ccccgtttgc
      361 tgtgccactt gccgaacatt ttgtttcagg tgattatatg acttctctcg agtgcttcac
      421 ggtactcgac acctgtgccg catcaataag ccacaatgac cgcgccagaa taatcatcac
      481 cctgcaggag aagaaggaat catgggaaac gccaaagcag aaacttgccg gtgagcttat
      541 cacattgctg aaaggataag gctgcaggaa cctgcaggac agctttctat atgtaggagc
      601 tgtaatgctt cataaactgc gaccgctcaa acagtgaggg attatcaaca ttcctgtagt
      661 tgagctttcc cctgaagtca cttatgcttc tgaactcctt gttgttcatc cacctggcca
      721 gagtctcatt gatctctgac acaacggtga atcccctctt gtagagggca gagcagacct
      781 gtacagtggt ggcccctgcc agcaggtact tgatcgcatc ctcacctgta tatacacccg
      841 tggaggccga gatgtcaatc ttgatatcct gggcactgac catacccacc cagcgaagga
      901 cataccgctg ttcagcggga ttggaaagca ccgaggcagg cacaatgtta aggttattga
      961 tatcaaagtc aggctcatag aacctattga acatagtcac ccccgccgct ccgtggttat
     1021 acatctgcca tacgaagttc aatatgtttg aaaacttctg gccgatttta acgctgaccg
     1081 gaatcttcag ctttttcttc aggttgtcaa ttatcctgaa atagaccttc tccgaatcgg
     1141 tgctgttttc gtgcttgtca gtgggaatga agaagatatt cacctccagt gcgctggcac
     1201 cggccctctc tatctcaagt gcaaaatcaa tccatccctt cggcgagaag caattgatgc
     1261 ttgctatcac aggtatcttc actttcttcc gcgccccggc tatcaggtca agataagcat
     1321 caacactgtt ggtgcgggta tagtactgaa ggtaatcgtc tgcttcgggg tatgattcac
     1381 gtgacgacat tgaatgggcc tgtgaaacaa tctgctcctc aaacagggat ttcaggacaa
     1441 ctgcaccggc tccggccgcc tcggcctcaa cgattgattc cacctttgag gtgaaattgg
     1501 aactgccgac aataatggga ctgctgaggt cgagtcccag gtaacgtgtg ttgattgtgc
     1561 tcatcttgct ttaaaaattc gttctccaaa tataagtgtt cctacgcgaa tcatggtact
     1621 cccctccccg acagccagac gccagtcgcc tgacattccc atggagagct ccctgaagtg
     1681 gcttttgccg gtgaaatact gcttacgcat cctgctgaag agatcggaaa gagacctgaa
     1741 ttcagaccgg accttctccc tgtcatccgt gaatgttgcc attcccatca gtccgcatat
     1801 ccgtgctgcc ttaagcgagt cagatacatg acgccagtct gtctctgcca tctcctccaa
     1861 tgagaatccc gatttggttt cttccgtggc gatatgaacc tgcagaaggc agtctgtaca
     1921 ttttccctgt gccagcgctt cggcatcgat cagccgcaga agcctgactg aatccaccga
     1981 ctcaatcaca gaggctgctg caacagcctg tttcaccttg tttgactgca ggtgacctat
     2041 gagatgccat gagatatcat caggaagcaa gggccttttt gtcatcatct cctgcaccct
     2101 gttctcacca aagatgcgga ggcctgccct gtatgcctca agagcctcct caggtgtccg
     2161 ggtcttggtt actgcaacca cgctgatgcc tgaaggcagc tcatcaagca acttcctgat
     2221 gttttctgca ataatgttat tcatggcgcc tgactaccat cagtcgagtt tatgaatcac
     2281 cagtccgctc ctgagcttgg gctcgaacca tgtggttttt ggaggcatga tgttcccggt
     2341 gtcggctatg gtaatcagct gtttcatgga tacggggtat agtgcaaatg ccaccttcat
     2401 ctcaccgctg tcaacccttt tcttcagctc cccaaggccc ctgatgccac caatgaaatc
     2461 tatccgcttc gaacgccgca gatcctgtat gtcaagcaga ggcagcaata ccagttcggt
     2521 aaggatagtc acatccagaa ccccgatggg atcatcatca taatacatac cctttttggc
     2581 tgtcaggctg taccatttgc cgtccagata cattccgaaa ttgtgaagtt tcgtcggcct
     2641 gtgcgtcctg gttcccttct ctctgacgtc aaatgactcc ttcagttttc cgaggaactg
     2701 cgcgggggtc ataccgttga ggtccttcac caccctgttg taatcgaaga tcttaagctg
     2761 gtcatccggg aaaataactg ccatgaaaaa gttgtactcc tcatcgcccc tgtgggccgg
     2821 attcttatct ttcttttcct tgcccacaag cgcagcagcc gctgtacggt gatgaccgtc
     2881 agcaacatac atataaggaa ccttctctgc aaaaagcttc tcgattttat cgttggtctt
     2941 cctgtcccat actacccaga agtgatgacc tatcccgtct tcggcaacga agtcatactc
     3001 gggtctctca ttcttcacga tcttttcaac aatcttgtca agttccctga cggaagggta
     3061 tgcaaagaac accggttcaa tgttggcatt gtttgcccgg acatggacca tccggtcttc
     3121 ctccttgtca ggacgggtca gctcatgctt tttgattatg cctgaaaggt aatcggtcac
     3181 tgctgcggcg cccacaatgc cgtattgtgt acgacctttc atggtctggg catagatata
     3241 gaaatgtctc tcgaggtcac caatcagcca gcccttcttc tgccaggaaa tgaagttctc
     3301 aagggctttc tggtatacct cctccgagtg cacgtcagtg ccatcgggaa ggtctatctc
     3361 ggctttggtg atgtgtaaca gtgatttttc tttacccctg gccatcacgg ccgcctcttc
     3421 ggagttcatc acgtcataag gaaggcaggc aatctccgat gccagattgg ccgggggcct
     3481 gagcccccgg aaaggtttaa caacagccat atctgaaatt tatctgtcta tttgggttta
     3541 ttaactctga aagtctggtc accggttgca aagaatgaaa ctatctggcg tgcagcagcc
     3601 actccggcat tgatgtttgc ctcttctgtc tgggctccca ttttcttcgg tgtaaagaaa
     3661 tagcggccgg caaacttctg tgcgaacagc tctgcattgt cgggggctac atcagagaga
     3721 tacttcaggt cgcttctctt ttccatcacg gcaaccagtt ctgcctcatt gatgacctcc
     3781 ttgcgcgcgg tattaaccag cactgctccc tggggcatca gcgacagaag cctttctcct
     3841 atggagccct ttgtcttttc atttgcagga atatgcaatg aaacatactg gcagccggaa
     3901 taaagagcct ccatggtggc taacggggta acgccggctt cctcaataac cttgctgtca
     3961 acaaacgggt caaaggcgca aaccttcatc ccgaatcctg atgcaatgcg ggccatgcac
     4021 ctgcccacag ccccgaaggc atgcaggccg agggtcttgc ccatcagttc ggagcccgac
     4081 ttgccgtcga agccaccgcg tgcgccatag accatcatcc cgaatgcaag ctcggcaaca
     4141 gcatttgaat tctgtccggg agtgttcatt gcaaccacgt tggctgccgt gcaggcattg
     4201 aggtcaagat tgtcataacc ggctccggcc ctgacaacta tcttcagttt tggggcagcg
     4261 gcaataaccg ctgcatcaat aatatcagag cggactatca ttgcatcggc gtcagctacc
     4321 gctgcatgaa gctcctgagg ggtcttatat ttctcaagga gggcaagtgt gtgcccagcc
     4381 ttctccgtca cctcacggat cattgttacc gcaacggggg caaatggttt gtcagttgct
     4441 acaagaatct tcatgtcgtc ctcctgtttt tttagtattt gtttgcaaat tccttcatgc
     4501 attctaccag cgcccttaca ctctcaatcg ggagagcatt gtagagagaa gcacggaagc
     4561 ctcccacaga gcggtgaccg gctatgccca ccatgccttt cgacttggcg aaatcgccga
     4621 actccttctc gagctccttg tagctgtctg acatgacgaa acagacgttc atcagcgacc
     4681 tgtcctcagg atcgggcacg gttacctgga acagtttgtt gcgctcgatc tcatcataga
     4741 gcaatgctgc cttttcctgg ttgatcttag ccatagctgc cagtccgccc ttctgcttca
     4801 gccactcgag ggtcttcaga gctgcaaaaa caggaagtac cggaggtgtg ttgaacatag
     4861 aacccttttc gatatgggtc ttgtagttca gcatcgtggg tatcgggcgg gtaaccttgc
     4921 caagagccga atccttgatt atgacgaagg tgacgccggc cggtgcaagg ttcttctggg
     4981 cgccgccata tatgatggaa tatttggaga catcaaccgg acggctgaag atatccgatg
     5041 acatgtcagc cacaagtggc accttcacat ccggatcctt tttccactgc gttccgaaaa
     5101 tggtgttatt ggtggtaatg tgaaaatagt caacgtccga gggaatgtca aaattcctgg
     5161 gtatgtaatt gaaattgcgg tcttttgacg atgccacctc gacaacctca ccgaaaagtc
     5221 tggcttcctt gagtgctttg cctgcccact caccggtatt tatgtaggca gccttcttct
     5281 cgagcatgtt catcggaagc atggcaaact gcagactggc gcctcctccg aggaagatga
     5341 cggaataacc tgcaggaatg tcaagaagtt ccctgatgtg actgactgca ctctccatga
     5401 cagcctcaaa ctccttgctg cggtgtgaga cctccatgat ggaaagtccg gttcctgcaa
     5461 aatccctgat agcttcaatc accttttcat ttgtgtactg gggcatgatt gaaggtcctg
     5521 cgtagaaatt gtgttttttc atttcctgag tatttatttg ttttagtagt ttttcgcgtc
     5581 tgtttctgcc gccggctgtg accagttgcg gcaaaggcaa tgctcagagg gcagtcagcc
     5641 tgtcccctct gagcaccggt atcacttctg ttacatcaaa tgtatgacaa taaggaatac
     5701 aatcatccag tcccttgtct ctgagcctcg atctctgggc tgccttctcc atatatccca
     5761 ggaggtcggt ctttgccatg ctccataggt cggtggcggc atgcacggaa tcacatacag
     5821 tgcagaattt cccgctttcc aggagcctcg cggccagagc gcctgcacat accgcatcct
     5881 caaggctgaa gcgtttcttc catccggcgc acacaatcac cactttacga ccctgttccg
     5941 caacccagtc acagagggca gacatgttga gccatgaacc tatgaccacg tcatggcaaa
     6001 cagaagcacg tttcatgatc ccggtgccgt tggtggtgct gtagacaacg ctcctgccct
     6061 tcaccttttc aggagtgaag ttgaacggag agttgccgaa atcggcgaag tcaagcacgt
     6121 aaccatcgcg ctcggcggca accaggtggc cccgggcttt cattgccctg gcttcttcaa
     6181 ctgtctcaac cggaattatc tccgtgactc cgttggcgaa tgccgtgcag atggaacttg
     6241 tagcccggag cacatctatt atgaccacca cagcctcgct gtcagcatga gcgtcaaagg
     6301 ctgcgggggt aaaacatatt tccaggctgt tctgtgtcat gggctcaaca ttccctggtg
     6361 aaattcactg tttgtagaat ggtaaggctg tgacctttgc cttaaggtcc ctccccctga
     6421 cacggatgaa tatctcagtg tcattcttcc agaagccctt gttcaggtat gccaggccta
     6481 tcccctgctt cagtgagggg gccatggttc cagaggtgac ctctcctatc aggttgccct
     6541 gtgcatcaac cacctcataa tgctgcctgg gaatcccctt gtcaatcatg acaaaaccct
     6601 tgagacgtct atcggttcct tcttccttct gtttcaggag gagatccctg tcaatgaaat
     6661 ccttgcccgg aacaaacttt gtaatccatc ccagaccggc ttctattggc gtagtggtgt
     6721 cattgatatc atttccgtaa aggcagaatc ccatctctag acgaagagta tccctggcgc
     6781 caaggccgat aggcttaatc ccatactctt cacctgccct gaaaacctct tcccagagac
     6841 ggggggcatc ggcattcctg atatagatct cgcatccgcc tgagccggta tatcctgtag
     6901 ttgagaagat aacatcatct attcctgcaa acttcagcac cttgaaggtg taatattcca
     6961 tgtcggtgac aggttcaccg gtcagcttct gcatggcttt gagcgccagc ggaccctgca
     7021 ccgccagctg ggcagttccg tccgaggcgt tgttcagctc cccgcccggg cctgccgtga
     7081 tgccgaaagc ggctgcatgc ttaacgcacc attcccagtc cttctcaatg tttgccgcat
     7141 tgaccacaag aagatacttc tcagcgctga agcgataaac gagaagatca tccacaatgc
     7201 ctcccctgcc gttcgggaaa caggtatact gtaccttgcc gtcagtcagg gcggaaacat
     7261 cattggaggt gatatgctga aggaaatcca gggcacgcgg accgttaacc cagaattcac
     7321 ccatatgcga aacatcaaat acaccaaccc cggaacgtac cgtgagatgc tcttccgtaa
     7381 tccccgcata ttcaacaggc atattgtagc ctgcaaaagg aatgatcctg gcaccgaggg
     7441 cctcatgata ctttagaaag ggagtatttt tcatatgatt aagcttttgg tttcatagca
     7501 aaagtaggca tttccggacg ataaaaagct gccataaatc atttttgcca ccggttcttt
     7561 aggtcgttac ccatgctgtg atggttgcat aatgactata tttgtaatct caaaacagcg
     7621 gagaaatgga atacagaata ttcaaacccg aatacctgct gagtatcacg ggaggtgata
     7681 tggagaccat gagcgagatt gccgggatat tcggcagcca ggtaccggag ttccttaccg
     7741 gcatgaagag cctgctcggt caggagaagt attatgaact cggtctgctc gctcacaagg
     7801 cgaaaggatc ggtgacggtc cttggcatgg atgaaacagc caaaatgctc aaggagtttg
     7861 aattgctggc aaaagccggg gatcagaagg agaaatacac cgggttcatc tccaggtttg
     7921 aagccgatac aagtacagtc atgaccgagg tggaagacta cctcaccaaa aacaggtgat
     7981 tgtttatggt ggttaccgac aggacagacg ggattgagat cctgagtttc aggaccgacc
     8041 gtatcaatgc tctcaacgtg gatgtaatca ggcccgttat cctgaaactg tttgagactc
     8101 cgtataccaa agttgtcata gacatggaag gtgtgagcta tcttgacagc acagcctttg
     8161 ccatgtttct ctaccttctc agggtggcac ggtcaaatta ctgcacttac aggctctgct
     8221 cactnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntctgcc agccaggcca
     8341 cagccttctc cgtaagttca ctgcttatgc ccttctcctc cagcatctga gccactcggc
     8401 tgaactgcat cattacaatc tcctttatct gcatgagggt caggggcctg aacatcacta
     8461 tctcatccac ccggttaaca aactccggcc tcagggttgt gcgaagcatc tgcaccagat
     8521 cagccctgag ctcctcagcc ttctcctctg tcattgcccc gctgctttcc tcaatgctct
     8581 cccggataag cgatgaccca aggttggatg tcatgataat tatcgtgtta cggaagttta
     8641 cagtccttcc cttgttgtct gtcagcctgc cgtcgtcaag cacctggaga agtatgttga
     8701 acacatccgg gtgggccttc tcgatctcat caaggagcac cacagaatac ggcttgtgcc
     8761 ttatggcttc cgtgagctgg ccaccctcgt cgtaaccgat gtatcccgga ggcgccccga
     8821 taagccttga aacgctgtga cgctcctggt attcggtcat gtcaatgcgt gtcataaggt
     8881 tctcatcgtt aaacagatat tcagccagcg ccctggccag ttcggtcttg ccgacaccgg
     8941 tggtaccgag gaatatgaat gaacccaccg gtctcttctc atcctgcagc cctgctcttg
     9001 aacggcgcac agcgtcggag actgccctta tggcctcatc ctgacccaca accctgtggt
     9061 gaagctcctc ctccagtctc aggagctttt ccttttcact ctcaagcatc cgcgaaacag
     9121 gtattcctgt ccatctcgaa acaatctcgg caatatcgtc tgacctcaca tcgtcgcgta
     9181 ccatggaacc ggcgttccgc ttttcagcta tctcggtatt cagcttctca atctcctttt
     9241 cattgtcggt cagtttgcca tagcggatct cggcaaccct tccgtagtcg cctgccctct
     9301 cggccctctc agcctcgaac cggagctcct ctcctgcctg tttctttttc tgcagatttt
     9361 ccaccaggtc cttttcggcg cgccagcgtg ccttgagctc atcacgccct gcagccaggt
     9421 cagctatctc ctttgagagc tccttctcct tgactgcgtc accgtcacgc ttcactgcct
     9481 cccgttctat ctcaagctgc ctgatctgcc tgtctgcggt atcgatctcc tcaggcaccg
     9541 agttcatctc gagcctcagc cttgctgctg cttcatcaat gaggtcaatg gccttgtctg
     9601 gcaggaacct gtctgtgatg taacggtatg acaggtccac ggcagagatt atagcctcat
     9661 ccctgatgat caccttatgg tgcgtttcat acctctccct gagccctctg aggatggaga
     9721 tggcatctgc cctgtcaggc tcttctatca tcaccacctg gaatcgtctc tcaagggctt
     9781 tgtcagtttc gaaatacttc tggtattcat tcagcgtggt tgcgcctatg gcgcgaagct
     9841 ctcccctggc aagcgccggc ttgaggatgt tggctgcgtc cattgccccc tcaccttttc
     9901 ccgcgccgac gagtgtatgt atctcatcga tgaacaggat cacatcgccg tcagatgcaa
     9961 taacttcatt tacaactgat tttagtcttt cctcgaactc acccttgtac tttgctccag
    10021 cgatgagtgc tcccatatca agcgaaaata tccgtttctg catcaggtcc tcaggaacgt
    10081 cgccgcgcac aatcctttgt gcaatgccct ccgcgatggc ggttttgccc acgcccggct
    10141 cacctacaag aatggggttg ttcttggttc tgcgcgaaag tatctgcagt accctcctta
    10201 cctcctcgtc gcggcctatg acagggtcaa gcttcccggc cctggcacgc tcattcagat
    10261 ctatggcata cctgctcagc gaatcatatg tctcctccga tgtctggctg ctcaccttcg
    10321 cgcccttgcg cagatcagct atcgccattg acagtgctgc tgtgtccatg ccgttatcct
    10381 tcatcaggcg ggagacactg tcggttgatt cggagagcgc cagcagcagg tgctcaacgg
    10441 atacaaacct gtcctgcatc cttgcggcat gatccgcagc acgcctgaat acccctgcgg
    10501 catccggaga aaaatagggc tctcccccgc ttaccctgga gtatccgtca atgatggcat
    10561 caacggcctt gccgaaagct gctgaattcc ccccggtctt ggacatgata tatgagacaa
    10621 tgctctcagc ctcagtcatt attcctttaa gcagatggcc acactcgata gcctgctgac
    10681 ccttgcccga ggcaatggtc acagccttct gaacagactc ctgtgccttt attgtaaaat
    10741 tctcaaaatt catcgtttca tactttttcc ctgttatgtt caatttattt gccaatcggc
    10801 cgcagaggct ttattcatgc cattatgaca gaccagggat aatatagaag gtgccattcc
    10861 gttatataag actcaggagc cgaaatgccg ttattgaaac ctctccggga tgaatgagaa
    10921 gatacttaat accctgatgc atctctttgc catcattgcc cccgcacagg gcaatgaaaa
    10981 tgacaggcgg ggggtggttg aggcatttct cagaccccag ctcaaccagg agggagttaa
    11041 ggcttacctg aagatctttg aggcatacta tgccgaggcc caggagagac tgaagaaggg
    11101 caaggaagcc cgccggaact catcgatatc tgtaaggatc atcaagatat gctttgacat
    11161 cgggatgcag ctgaccctca accagaagat catcgtgctg gtccagttgc tcgaatactg
    11221 tcggtctgac cataaggggg taagcaacac cgaacttgaa ttcatcatta ctgcagctga
    11281 aggcttcaat atcaacccgg aagattacca gttgattcag caattcgttc tgtcatcaaa
    11341 ggatgagatc cctgaatcgc cacagatact tgtcattgac agtgacaggg tcactcaata
    11401 tgagaaggcc caccacattg tcaacgactc attcaggggc caggtcaggg tgctgaacct
    11461 gccgcaggct gacatgctct tcatcaggtc attcggtacc ggggagctcc tcctgagcgg
    11521 ccagctgaga catgaagaca gggtgtacct cttcgagagg ggcgcttcac tcaagttcat
    11581 gagcagcaaa ccaatctact actcagaggt cataaggcaa ttcctcgacg aaagcgccct
    11641 ggcttcacgg gtggtctacg aggttaatga gattgaatac aagttcaagg gcggacaggt
    11701 aggcctgcac cgcatgagct tcgcggaaga gtcaggaagg cttatcggga tcatgggagc
    11761 atccggggct ggcaaatcaa cccttttgag cgtccttaac ggcaccaaca ggcccgactc
    11821 gggcgaggtg ctgatcaacg gcatcaacat acacaatgac cccgataaga tcaaggggct
    11881 gataggattt gtgtctcagg acgacctcct gattgaagag ctgactgtat ttgataacct
    11941 gttctataat gcaaagctct gtttcggcaa cctggatgat gaggctgtca ctcataaggt
    12001 ggatgatgtg ctgcacagcc ttggccttta tgatattaag gacatgaagg tgggaacccc
    12061 gctcaacaag aaaatctcag gaggtcagcg caaacggctc aacatttcac tcgagctgat
    12121 cagggaacct gccattatgt tccttgacga gcccacatca ggactctcgt cgctcgattc
    12181 agagaatatc cttgaccttc tcaatgacct gaaactcaag ggtaagctta tattcgtcgt
    12241 aatccaccag ccctcctcag acatcttcaa gatgtttgac aggctcatct tccttgactc
    12301 tggcggatac atggtctatt acggcatccc ggtaagggcc attgactact tcaaggacag
    12361 ggtccatctt ccgcgataca atgacagtga gtgtcatgca tgcgggaatg tcaacccgga
    12421 acagatcttc agcatcgtgg agtcaaaggt gcttgacgag ttcgggaggc cgacgatgac
    12481 gaggaaggta tcccctgccg aatggagttc ctatttcaat tcccgccgtg aggagcgtcc
    12541 gccgcatccc aggtccggca acggcatacc tgagataaca cttcgtacac cgggcaggct
    12601 caaacagctg gcagtgtttg tcaaacgcga cattcttgcc aaactctccg actcacaata
    12661 cctgatcatt acccttctcg aagcgccggt actggctctg ttcctggcat ttatcattcg
    12721 atattttgat gaaagcgtaa gtaagcccgc ctataccctg attcagaatg acaacctccc
    12781 ggtttatatc ttcatgtcgg tgattgtggc aatattcatg ggactgacag tcagtgcgga
    12841 ggagatcatc aaggacagga agatcctcaa gcgggaggcg ttcctcaatc tcagctggaa
    12901 cagctacctg ctgtcaaaaa ttggcgttca gttcctgatc tcggcaatcc aggccgcatc
    12961 attcgttgcc gttggaaact caatattcgg catccgcggc atgtggttcg aatactggct
    13021 tgtgctcttc tcatgctggg caacgtcaaa catgatggga ctgctgatct ctgacagttt
    13081 caagacagtt gtcacgattt atatcctgat accgttcctc gtcatacctc agatcattct
    13141 tagcggcata attgtaaagt atgacaagct taatcccacc atctcgtcac ccgtctcgat
    13201 cccggtttac ggtgagatca ttgcagccag gtggggttac gaggcgctgg ccgtagacca
    13261 gttcataaac aacaggtttg aaaagcagtt ctacggttat gacaaggcca tcagtaccgc
    13321 caagttcagg aaagacatct ggtataatga gatgaaaagc atcatctcca ggctggagag
    13381 caacctcgag agagagaggc ttgatgaatc gtcaggaaat gacatgatgc ttatcaggaa
    13441 cgagattgag gaggagctgg gctttaccac agccgtaccg tttgacctgc gcatcctgaa
    13501 cccggatgcc atcaccgctg aggcacttgc tgcggcacgg gattatatag agagagtcag
    13561 gcgttattat atcgaagtgt caaggaacgc tgtcgaagcg cgtgaccggg ccatcatggc
    13621 ctatgagcag gctgacaggg aggcctttat cctgatgaag aaacagcaca ccaatgaatc
    13681 tctcgaggaa tttgtcagga acgacaatga aaaggacaag ataaagcgct accgtgacag
    13741 gctctaccag aactatgacc agattttcta cgatccggcg cacccgctgg tgaaggcgca
    13801 cttctatgca ccgcagaaac aggtattcgg gctctttgcc ggcacgctgg cagtcaatac
    13861 ggcagtcata tggttcatga ccctctgtct ttacatcctt ctctatttca gggttctgca
    13921 ccgcatcctg gattcttcgg agaagttcat cagggtgatg cgcgcgaagc gggaaagagg
    13981 ctgaaggtaa tattataagg gggtatctgc ctgaagacag ataccccctt tcactatcgt
    14041 aaccgatctg acgctgtgcc gaagcggaat tactcgacta tctcaatcat cttaaaaggc
    14101 accttgatga tggaatcata gtcctcgcct gcctccagca tatcctcatc gtcctcttca
    14161 tagaaccagt tgatttccac gtcatgcttt gacctgtgaa tggcttccag gcgcttgaag
    14221 acgtcaagta tgcactttga ggagctggta ttgaaatatt caaggcggat gttgaccacg
    14281 gtcttgtcaa gcggagtctg catgtactgg tcgagccact cattcagcgg cttgtagaat
    14341 tccaccgagt tctcgggtat tgacctccct tttatttcaa atacaccatc ctgtgtatca
    14401 aattttatcg acggtgtctt cggtgttcct tcaataatga tcggttccat aactatctgt
    14461 tttttgttaa tctatctata cctgtgccac cactgcagtg agccggaagg cagtattcag
    14521 gttatcgtat ttcttgaacc tgaaatcaat gcgatggcct gtcttcctgg ctatgtcaag
    14581 caaccccagt ccgccaagcc ccttctccgt tatctcctgg tggttaagga tatccttgta
    14641 ctgttcctta atctcttcag gagtagaacg gttaatccgg gtcagcttct cctcaagctc
    14701 tttaactctc acagcggcga caaaattgca cgttgtgatg cgataacccc tgccgatttt
    14761 ctccatcacc accagcccga acctgtcagg cctggcagca tggtaacctc tcggcacctt
    14821 gtctgagtga tggtagaggt tctgaaggct ctcaacaagc acattgtaaa ccttctttct
    14881 cagcctgggg gcttcacgcg tcgcaaacag ctttttctcc acaaggtcca atgccttcgt
    14941 tatcgtgtcc gaatcaacat ttcccttgta ataaaggact gctcctctga tgtcaaggtc
    15001 gctcacaaga ttttcaaggt caaagctcat cgggcctctg tgcaagtatc caaagctaga
    15061 acgagtaaca aaaatagatt attgttgaca aaagccaaca taccgtctta ttattttgga
    15121 tatgacaacc tttttttgaa tttggttgca acggtggtaa caaaacctgt ctcaggcgcc
    15181 ggaaacatct ctgtttttcc tggttcctcc gtagagttca tagccctgga aacggcactc
    15241 aatatcgccg ttgagaaggt caaacttgcg aaaaggctta agcgctacct tcttgagcga
    15301 gaccatgtca cccgacagga tccaggcagt gtaaccctcg taactgtgtt tgagcacgga
    15361 accgatcctg ccatagaatt catccatatc cggtgtcccc aggcggcggc cgtatggcgg
    15421 attgataatc agcgtcccgc ttctggccgg aggaggtgat gcaaaaaaat cacgctccgt
    15481 tatctcaacg gtatcctcca ggcctgctgc cctgacgttt gccctggcaa tggcgcaggt
    15541 atcagcagat atgtcactgc cgaaaatcgc tgccggtgat tttatttccc gcttctcggc
    15601 atcgaacctg actgacctgt agagtgcagc atcataatcc ttccacctca tgaaaccgaa
    15661 acttttcctc atcttccccg gagcaatatt gcatgagatg aacccggctt ccgtaactat
    15721 agtacctgat ccgcacatgc catcagtgag aggcgttccg ggcttccagc ccgatagggc
    15781 tatcagcccc gcggcaagca cctcgctcaa cggagcctcc gacattccct ggcggtaacc
    15841 tctcctgtaa agaggaacca ccgtagaatc aagtgatatt gtcaccctgt tgttgctgat
    15901 gtgcacgttt accagcaggt cagggtcctt cgcgtccacc gacggcctcc cgagtgtcag
    15961 cctgcggaag aaatcggcta tggcatcctt caccaccagg gctgcatatc ccgtgtgggg
    16021 aaaatgggga gaattgacaa ctgccgtcac tgcgaacgtt ttgccggggt ccaggtaacg
    16081 gtcccactca acggctaccg ccccgttgta caggtctttc tggccggaaa taccgaacga
    16141 tgatacaggc atgagcaccg acagcgcaag acgactgcgg taattgacgg tgtacaacag
    16201 cagctttgac ccttcaaagg caacagccct gttgagcacg gtgacaccgg cagcgccaag
    16261 gctcttcagt tcttcggcca gaacctgctc aaggccatag tgagtctttg caagaagctt
    16321 catcactgtg gtttacgata tccgagaagc gtctcgtaaa accgggaatc attcagcact
    16381 tcaagcgcct tgctgattgc cggatcattc tcattgataa ccctgaaata ctcattcata
    16441 tcccagatgt cgcgggctac tagagccttc atcaccgatt ttatctcggg ggcggataca
    16501 tcatactgct tctggtcaaa cctgacgccg ttcttctccc ctgccgcctt cagcccttca
    16561 atggctgcag cgtcaatctc atactccctg ctgaatgcat caaagctcct gtacctccgg
    16621 gtaagctgtg accggttgcg gtcagtgtat gaaagcatgt agttcgtgag agctgaggtc
    16681 ctgataaggt cgcggtaata atcggaataa taagtagtat caataggtat gaagatatcc
    16741 ggactgatgc ctcctccgcc atagaccacc cgtttgttcc tgatggtgaa ggccttgagc
    16801 gagtcaggca ggttaaggct gtcaggatgg atcagctctc cgctgaaata cctgctgtag
    16861 aatgtctgga aatacttgtc gaaaccctcg tcatacggag tctggatgga gcgacccgaa
    16921 ggtgtatagt atcttgcaat ggtgagcctg atctccgaac cgtcaggaag cgggaacctg
    16981 ttctgaacaa gacccttgcc gaaactgcgg cgcccgataa tcacaccccg gtcccagtcc
    17041 tgcaccgcac ctgccagtat ctcactggct gaggcagagc cctcgtcaag cagtacggca
    17101 acacgtgccc ttgtaagctc tcctgacggg gcactctggt attcctgcct cgatgtatgc
    17161 agtccctcga gatatacgat cagttcttcc cccgaaagaa gctcatcggc tatctgaacc
    17221 gcagggacca tataacctcc cgagttaccc cggagatcta tcaccaggtg ctccatgttt
    17281 cccgttctca gcttctcaac tgcctgcgaa aactcaacgg cagtctgctc tgagaaacgg
    17341 ctgagcttga tgtaccccac atttccagtc accatatatg ccgcatcgag actgttaacc
    17401 gggatcttgt cacgggtgat cataaaatcg agcagttcct tctctccttt cctgaagatc
    17461 agcacatcaa ccttcgttcc cttcggtccc ctgagcctct tctgcacccc ggtggtggtc
    17521 attcccactc cggtaacctt ctcaccggca atggatatta tcctgtcgcc ggccatgatg
    17581 ccaacctttt ccgacggtcc tccggggatg ggtgagataa ttataattgt atcattgagg
    17641 aggttgaact gaattccaat cccttcaaaa ttgccctgga gcggttcatt ctcttcctgt
    17701 acatcctttg caggtatgta taccgagtga ggatcaaggt tacggagagt gcttattatc
    17761 atctgctcgg tcagggctga aatgttgacg ctgtcaacat atattgcctc cagcagggca
    17821 agagtttttc ccagcttgaa tgtctcctgg gtgagcccct gcgacctgag agaagtgcac
    17881 tgcatcagca gggcggcaat tactgttact ataagtttct ttgtcatttc cgttttttct
    17941 cttgacttca ctgcaaattc cgttcccaat gtttcggcgg cgtaacgggc ctttcacggg
    18001 gatccgcagg cttccgcccc ggttcccgga tcaggagttc attcccattc aattgtagag
    18061 gggggtttgg aactgatatc gtaaaccacc ctgtttactc ccttgaccct gtttatgatc
    18121 tgatttgaaa cctctgccag gaattcatgg ggcagatggc tccagtcggc tgtcatgccg
    18181 tcagttgatg tgactgccct cagaacaacg gcattctcat aggtgcgctc atcacccatt
    18241 acacccactg actgcacagg cagcagaatg acggctgcct gccagacctt atcatagaat
    18301 ccgtttttct tcagacctga aataaatatc tcgtcggcat cgcgaagcgt ctccagcttg
    18361 gacggggtaa cctccccgac tatccttatt gccagcccgg ggcccgggaa tggatgtctg
    18421 ccaagtatct cggcgggcat gcccatcgca ttgcctaccc tcctcacctc atccttgaac
    18481 agcaaacgca ggggctcaac taccttcaga tgcatcttct caggcagtcc gccaacattg
    18541 tgatgcgact tgatggttgc tgaagggccg tttacagaga ctgactctat gacatcagga
    18601 tagattgtac cctgggcaag ccatttgaca ttctgaacct tgtgagcctc acggtcaaaa
    18661 acctcaatga aaatgcggcc aatgatcttc cgtttggtct ccgggtcatc cacgccgcgc
    18721 agtccttcca caaactcagc cgatgcgtca acacccctta cattcagacc catccgtctg
    18781 taggtgtcaa gcactgactc atattcatct ttacgcagca gaccgttgtc aacaaagatc
    18841 gacgtgagcc tgtcgcctat agccctgtca agcagcaacg ccgcaaccga tgagtccaca
    18901 ccacctgaca ggccgaggac caccctgtca tcgccaagct gctccctaag ggaattaaca
    18961 gtcatttcca caaatgaccc tggtgtccag tctgcattgc aggcacatat gactcttaca
    19021 aaattacgca acatcgcagt tccttcagtg gtatgatata cctccgggtg aaactgtatt
    19081 ccccaggtat tttcacccgc tatcctgaat gctgccactc tcacgtcttc tgtcgatcct
    19141 gtgataatat atccctccgg gactcgcatt atggtatcgc cgtgtgacat ccagacctgg
    19201 gtcccggggc tgatcccgtc aaagagcggg tcagaggtgt caacctttac caatttcgcc
    19261 cttccgtatt cacgggtatc tgatgccatc acctcgcctc cgtatccatg ggtaagatac
    19321 tgagctccgt agcagacacc gaggagggga agcctgccct taatcccctt caggtcaggg
    19381 aagggggccc ttgcatcacg cactgaccag gggctgcctg acaggatgac tcccttcacg
    19441 ctgctgtcag ggagagggaa atgattaaag gggtgaattt cgcaatacac attcagttcc
    19501 ctgacacgtc tggcaatcag ctgtgtatac tgggatccga agtcgaggat caagattttt
    19561 tcctgcacct gtgatttatt aaggcggtta tcaaggggtc aaagataatt aatctgctgt
    19621 tacccggtta tcagccgatc tcatatgtgt taccttcccg ggcagcttcg gtatcaggaa
    19681 agatggtgcg tgcctcatcc tccagcaatg ctgttgaccg gtaacgggca gaaaagtggc
    19741 cgagcaacag cctgcctgct cccgcttcca gcgccacctt cgccgcatgg ctggccgtag
    19801 agtggcgggt ttttaccgcc agttcattat tgtcggcccc gaaggtggct tcatggtaga
    19861 gaagatcaac accctttacg tatgacggca gttttttaaa ccatcctgta tcactgcaga
    19921 aggcatatga ggccggccgg cggggctcca gggtgacccc ggcacatggt atgatttcac
    19981 cgttctcccg tgttatatcc ccaccccttc tcgcggtggc aatctcttca attgaaagtc
    20041 cgtattccgt gatcttctcc ttaatcatat tacggtcggg catcttctcc ctgaaaaggt
    20101 acccgtatgt gggtatgcgg tgcttcagcg gaaaagagaa tacctccacc cgtttgtcgt
    20161 caagaacctt cttcagtgtg cgccctgtga cagtgtgaac ggcaaggtca aatccgaggg
    20221 tgatgtcaaa atcagccaga tgcctggtta tcagctcctc tgccggagca ggggcataca
    20281 ggttcagcgg tattttcctc cccatcaggt tataggtgga aagcagggga taaatcccga
    20341 agaagtgatc accgtgaaga tggctgataa aaatatggtt gatccggccg aaacggatcc
    20401 tgtagcgtcg catctgcatc tgagtgccct cgccacagtc aattaaaaat aaccgctcat
    20461 gcacattgag tacatgagcg gtcggatatc ggtctgaggt gggcagagca gagcttgtac
    20521 ccagtatggt cagcctgaat gccatgctgc cgcctcttct caggagatga gtttctctgc
    20581 ctcttcggta tcagcggtta tggagagcac agtatcaagc tgcgagatgg ttataagtct
    20641 ctcaacagcc tcgttcagcc cacacagcac aaaagtaccg ccggcgttct tgcagagcct
    20701 gtttgccacc agaatggcac tcagacccga cgaatcgcag tactgacact tcttcaggtc
    20761 aagaatgatg ttcctctctc ccttgcctga gataagtacc agctccgact ttagagaagg
    20821 tgcaatatgg gtatcaagct tctcagtctc aatgcttata acagtgcact gctttttgtt
    20881 gtcaatattg aaatccatag ctgattattt tggcaactaa aaataaggtt attttttctt
    20941 tttacctaga cttttagctt ctttttcttc catctcatcc ttgagggcct gaagctcagt
    21001 gatgtctccg agagtggtct tctcaaggct ggccttgatc ttgcgggctg ccttcttggt
    21061 gttgtcagcc tcgctgcgcc ttgaagcagt ttccgaaccc tttctttcat cctcgaagac
    21121 gcggctgtga gaaacgatga tcttcttggc tcccttgttg aactcaatca ccttgaagtt
    21181 gagcttgtct tcaacagcgg cctgggtacc gtcttccttc acaagatgct tcggagttgc
    21241 aaagccttcg acgccgtaag gcagcgctat gatcgcgccc ttgtcaaaga cctcgataac
    21301 ggttccttca tgaatggagt caactgtgaa gagatcttca aacacatccc acgggttctc
    21361 ttcaagctgc ttgtgcccga ggctcagcct cctgttctcc ttgtctatct cgaggactac
    21421 cacgtcaatc tccgcatcga tggcggtgaa ctcggcagga tgtttgatct tcttcgtcca
    21481 tgacaggtct gaaatgtgaa tcaggccgtc aacaccctct tcgatctcaa caaatacacc
    21541 gaagttggtg aagttgcgga cgcgtgcctt gtggcgtgag ttgatgctgt atttctgttc
//
LOCUS       DAOPXU010000044        21257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_2544_length_21257_cov_13.387982, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000044 DAOPXU010000000
VERSION     DAOPXU010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21257
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2544_length_21257_cov_13.387982"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PKK03_06125"
     CDS             complement(<1..1010)
                     /locus_tag="PKK03_06125"
                     /inference="COORDINATES: protein motif:HMM:NF017669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNX84012.1"
                     /translation="MSRKDDFFSSINAGYTCKGDFIVLGGAMLDGEAIADAHIKIPLK
                     TLNRHGLIAGATGTGKTKTLQVISEQLSLKGIPSLVMDVKGDLSGLARPGQENDFIRE
                     RHGKINLPYSTMGFPVELLTISEQNGVRLRATVSEFGPVLFSRILDLNDVQAGIMSII
                     FKYCDDNQLALIDLKDIRKVIQYLTGEGKDELEKEYGAMSTASTGVILRKIIELEQQG
                     AERFFGEESFDISDLMRRDSDGNGYINIIRLTDIQDKPKLFSTFMLSLLAEVYNTMPE
                     KGDVPKPELMIFIDEAHLIFSQASKVLLEQIETIVKLIRSKGVGVYFITQNPMDVPAS
                     VLA"
     gene            complement(1086..2192)
                     /locus_tag="PKK03_06130"
     CDS             complement(1086..2192)
                     /locus_tag="PKK03_06130"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNX84013.1"
                     /translation="MKKTVILLLVVVVAAACAREPKFTLTGTIDGAGNETVILQKRIP
                     GGYQVIDSATIENGTFKIEGVVDYPQMVNLGIRGKRGGLSFYIENSDITVHGHADSLY
                     MGSVSGSATQAEYDSYKALLDETNAEMTKIYDSYRKARMDGNEELAASLEKEIEAIDA
                     KQTNIKKEFLANNPSSFVAPAVLSELAYYLEVDEMEGILNKFDTTLNKVQTVITLKDR
                     LVQLKAVAVGQKAPDFTLNDPDGNPISLSSKIGGDTKLLLLDFWAAWCGPCRQENPNV
                     VKVWAEYNKKGFDVFGVSLDRSKEDWLKAIQDDKLTWTHVSDLKYWDCAPAKQYAVNA
                     IPANFLIDANGIIVGHNLRGDALAEKVKEILDTK"
     gene            complement(2255..3355)
                     /gene="meaB"
                     /locus_tag="PKK03_06135"
     CDS             complement(2255..3355)
                     /gene="meaB"
                     /locus_tag="PKK03_06135"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNX84014.1"
                     /translation="MSRERRTAGALSVQQGVPQPPSINPDMLNRLKRNRRKMLSADDY
                     VDGILRGNRTILSQAITLVESNLTDHYDLAQEVVERCLPASSRSVRIGITGVPGAGKS
                     TFIETFGTMLTRHYGRRLAVLAIDPSSQQSGGSIMGDKTRMEELSTDPDAFIRPSPSA
                     GTLGGVARKTRESIILCEAAGFDTILVETVGVGQSETAAHSMVDFFLVLMLAGAGDEL
                     QGIKRGIMEMADTIVITKADGNNIQKAEMARVEYKNALHLFPPAPSKWTPRVMTCSSL
                     ERTGIADIWEAIRDYVSVTKSNGWFWDRRREQAVTRMHETIIDTLKQSFYTNPDVAAM
                     LPELEKKLREGEVTSYKPAALLLDKYFDSSCK"
     gene            complement(3379..4470)
                     /locus_tag="PKK03_06140"
     CDS             complement(3379..4470)
                     /locus_tag="PKK03_06140"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNX84015.1"
                     /translation="MKRSISFLVLMLACAGMMAQPKLNFANTRHDYGTIKEEAGKQEA
                     VFNFTNTGDSVLVLTRVQSSCGCTAADYTKSPVPPGGKGFVKAVFDPKGYSSRFAKSV
                     TVYSNAKPAVTVLVIEGNVAPREKTVEELFTFAVGPVRFQSNHLAFTTTTKSEKKIRV
                     MPVINTSKEPATVEFEGLPAHLQLKVSPATLKPGEKGIIEGTYDAKLNSSAWGNVNDL
                     VRLKVNGQSQPNIYLYVSANLVEDFSGLNAQQLASAPVFKLDSNTVNFGKMKQNSSAD
                     VEYFFTNQGKSDLIIRHVKAGCGCTALMPTENVIKPGARGSIKAKFNSGGYKGQIYKN
                     IFVYTNDPKNSEVMLMIKGEVLVDEASNK"
     gene            4690..7965
                     /locus_tag="PKK03_06145"
     CDS             4690..7965
                     /locus_tag="PKK03_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84016.1"
                     /translation="MIRKGLYIPFLTLFFLLTVAPASGQFYNGMQMTFGKNRVQYKDF
                     YWTYFRFDEIDCYYNEFGREVAEYACKVAYEKLNEIEDYFDYTIDKRPILIIYNKKND
                     FRQSNIGLISAEDDYNVGGYSRVIKNKIMLWFNGDHEEFSKLIASAIAEVVVYEMLYN
                     ADLRDRTTSGSQIQIPDWYIKGLINYVAWGWDSDTDNRVRDGFENNRYKNLDNLEYEE
                     AIYAGHSFWKYIGDTYGDAIIPNIIYLSKVYRNIEDGFLYGLGKSIKDTYRDWRNYYS
                     AYYETKPGENYQVPREVKKAKPYERFTDVKVSPDGRYVAWVSNDWGKRRIWLHDTSTG
                     KSRKIFTAEPKFEQSVDHTYPVLTWHPGGQILTFANEEKGGVQLYFYRTAEKKFEQRT
                     MPFFEKVLSLSYSPDGSRLLASAVINGMTDIYVHTIISGTNERITFDIADDLNPLFVK
                     GSDEIIVFSSNRVTDTLTNRGDPQERTGLTYNLFTYNLGAADGKLTRLGEDSMQDLMK
                     PVMPTPSSTGFIGNGNGIMNHYTGNIDSTIAFIDTALHYTYFITPRQATDFPRNLEAF
                     DRSGGQDAGVIFNQGRYRIFTGPSSATPVADTADIKTTTWRLEEIAMLRAADSINRLR
                     DWLLAERARMNDTLKKPLYEYYTPANEPIDFRNYVFEKEKENFYELQWRKDYMDINLD
                     TSRLKMPDALVYRPAFYNNYTATQIDFNFLNNTYQAYNGGAPYFNPGLNMLFKIGTLD
                     LFEDYRITGGFRFSGNFDSNEYLVSLEALRGRFDKQVIFHKQSFDSYNDTSYYKIKSY
                     TTYMSLRKPLSAVLALKGTLTYRYDSYVVQSTDQASLIAPSFNRHWAGLKGEVIYDNT
                     RKRTINIYYGTRFKIFGEYYRELTEKKSDMFVLGADIRHYQKIHRDLIWANRFAASTS
                     FGPSRLIYYMGGVDNWMRLPFGNVPQFDQTVPVNPNVNYGFQALATNMRGFTQNIRNG
                     SNFALINSEIRWPVVRYFAGHPLRSNLLNSIQLVGFYDVGMAWSGWDPWGNENYWNDK
                     VYRNGPVVVTIDAMREPLVMGFGGGARAQLFGYFIRADLAWGIDNGYILPKIFYLSFS
                     LDF"
     gene            8148..8315
                     /locus_tag="PKK03_06150"
     CDS             8148..8315
                     /locus_tag="PKK03_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX84017.1"
                     /translation="MAYKISDDCTACGTCIDECPVEAISEGDIYKIDPDICTDCGACA
                     DVCPVEAIHPA"
     gene            8513..9001
                     /locus_tag="PKK03_06155"
     CDS             8513..9001
                     /locus_tag="PKK03_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNX84018.1"
                     /translation="MQQRDRAMLGIYYGCGLRKSEGTNLITGDILTERKLIFVRKGKG
                     CKERYVPVTDGNLSYITEYLQDGRNFLLARNQSGTESFFINQFGGSCSDQALSARLDR
                     LVKNSASTGLQGKKPTLHTLRHSISTHLLQSGMEIEMIQKFLGHASLESTQIYTHILN
                     EC"
     gene            8995..9441
                     /locus_tag="PKK03_06160"
     CDS             8995..9441
                     /locus_tag="PKK03_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase SAM-like domain-containing
                     protein"
                     /protein_id="HNX84019.1"
                     /translation="MLSEAKSRKAGSFKEYRQKKGYSEKSIRVQDSHVNSFKSWCIRE
                     NINPSDITYNQTLKFIDSEKERGISRQSIIREINSIRIYFDYLMESGMTHQNIIKRIR
                     IRQNGKKVLPEILSTLQLEKIYQDFSGLPAWNHGTATAKLLHQRTQ"
     gene            9453..9911
                     /locus_tag="PKK03_06165"
     CDS             9453..9911
                     /locus_tag="PKK03_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNX84020.1"
                     /translation="MIYQGLDSGEIARLETNHVNLNEGKIYIPSGRKSSSRTLKLQAN
                     QILPLKTYLEETRPAILEKRELQSTYLFPSKKSSDLVCNIVEAVKKIHPEIKDSRQLR
                     SSVLMNWLKTNNIRQVQYMAGHKSIRSTESYRREDLTDLTKQLEMFHPLR"
     gene            10031..10570
                     /locus_tag="PKK03_06170"
     CDS             10031..10570
                     /locus_tag="PKK03_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HNX84021.1"
                     /translation="MIPDEVVMSKIYVIRGVNVMIDRDLAELYGVETKYLKRQVKRNI
                     IRFPEDFMFELTNQEFNEWRSQFVTSNEDRMGLRYAPYAFTEDGVAQLSTVLNSERAI
                     KVNLQIIRMFSKLRRLTLTHKDILHKLEELERNDIEHDKKIELIFEYLKQLEAAKQQE
                     LEQKNRKRIGFKTPGSKED"
     gene            11228..11875
                     /locus_tag="PKK03_06175"
     CDS             11228..11875
                     /locus_tag="PKK03_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84022.1"
                     /translation="MLENDPYWETYFDNPYTSSYATGSSYYSNYSAIYGKQPENPHRF
                     TSGYWVREWVDSNGNIIYPDGLSGMGYFPEITIVERFIPMAQVEGVMERARDPWWYEP
                     ADFLTTYLVSLDVLARLSGGLKEMPGCGQVGFAVQTVKSGHKILTGDNSIFPYTDFTA
                     SFIGAFVGLGGFIGGINYLLMDKICGREEFAEGFINAVNLYSEYGNPMFIRSWTH"
     gene            complement(12352..13380)
                     /locus_tag="PKK03_06180"
     CDS             complement(12352..13380)
                     /locus_tag="PKK03_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84023.1"
                     /translation="MTHFSRIITAAALVLLTVFSASCRRDPYRVNVSGIELDLRIRDL
                     GSEVFSTPPNELAALADTLKLEYGRALDTYSTVIGLGDPSDERWNSAFVLFATDLRNL
                     DLWDTVKKVWPDTERLEKELESAFRHYRHYFPDRVVPQVITCISVFNNSIIVDDSLLM
                     VSLDRYLGAGCAYYPSIGIYSYQARKMTPDYTASDCMYAWAATEWDYNSAGYGTKTLL
                     NSMLHEAKLVYFTRRMMPALADTVLFGFTGKQMEFCKEGEGMIWEYLVSKDLLFSTDG
                     FMIRKFTGEAPFTSYFTEESPGRAVVWTGFRIIERYMNNNPDVTMEELMSMTDCQAIL
                     AGAKYNPK"
     gene            13457..14155
                     /locus_tag="PKK03_06185"
     CDS             13457..14155
                     /locus_tag="PKK03_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX84024.1"
                     /translation="MKRVINRTVSRTMILVLMAAMPARAVAQELVFVVHADPVISWIG
                     SNESEYASEGARAGFDIGLNVLHYFAENYAISSGISIISAGGRQSATENHTMVFNNLD
                     PPTIPAGDEMKYNLKYMNIPIGIRLQTNQVGYLTYYTDLGFDVRLLLKSTVDLPTLQI
                     FSENAKNEVFGMNAGWHIGGGVEYELGIDASLIAGLTYAQDFFDITKDLKDVNQHPDR
                     SGLRMFRIRLGLKF"
     gene            14166..15827
                     /locus_tag="PKK03_06190"
     CDS             14166..15827
                     /locus_tag="PKK03_06190"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNX84025.1"
                     /translation="MKVALAQLSYIPGDIAYNKSKILNALEEARRQGADLAVFSELAV
                     TGYPPLDLLLRSDIIDSSMAAIREIATHCNGIAAIVGGPSPNTGEYGKSLYNSAFFLQ
                     EGKINAVVNKALLPTYDIFDEDRYFKAGNTFRPVELNGQKIAVTICEDIWDEQPFGDR
                     GLWRLYSVAPLDEIMRQHPSLIINIAANPFSHNRLRVREEVFVRNAREYRVPLISVNQ
                     TGGYTELIFDGSSVLIDSDGTIIDKLGFCTEEVRTVEIPSPGTHRSSLSFPPPLPDDM
                     TGLIHRALVTGIRDFFDKGGMKKAITGLSGGIDSAVVTALAAEALGPENTLALLMPSV
                     YSSGHSVSDSVKMATDLGVPYHIVSIEDARQALEKTLQPFFAGKAPDVTEENIQSRLR
                     AVILMAFANKSGYMLLNTSNKSETATGYGTLYGDMAGGLSVIGDLYKTEVYRLAREIN
                     SHAEIIPQAIISKPPSAELKPDQLDTDSLPPYSQLDPVLYRHIDLEWPADRIIADGFD
                     KELVEKVIRLVRTSEFKRKQTPPILRVSSKAFGTGRRIPIIAVRS"
     gene            15939..16640
                     /locus_tag="PKK03_06195"
     CDS             15939..16640
                     /locus_tag="PKK03_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNX84026.1"
                     /translation="MLYTNPYLEQCIEGPFSVFRDLPDKEKEFLIQDHSCITLKKGEM
                     IFHEGNRPAGLYCLASGKVKVYKEGIGGREQIIRMVRPQGLIGFAAFISGNAYSASAV
                     AIEDSGICRFDRDPFMKLIRRNPDLSMKLMRMMAVELSFSNGRTISLTQKHIRGRLAE
                     SLLVLRDTYGVENDGRTIRAYVSREDLACFSNMTTSNAIRTLSMFASESVIALEGKKI
                     SIVDQARLEKISQLG"
     gene            complement(16641..19097)
                     /locus_tag="PKK03_06200"
     CDS             complement(16641..19097)
                     /locus_tag="PKK03_06200"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HNX84027.1"
                     /translation="MIIGARELAHITGGTLTGDENLNVTGVMTDSRSPGAGPDMLFIA
                     LRGPNHDGHKFIEPLYARGVRLFLIDSLPSGHGEMAGAAFVKVTDTLYALQCMAKWRR
                     ESFRGRVIAVTGSAGKTIVKEWLAGVMSTSRSVIRSPKSYNSQVGVALSLLNLDDSYE
                     IAVIEAGISRPGEMERLARIIMPDVVIITNVGDAHGENFSGREAIAAEKLRLASGAQL
                     LICNADERIIRKEMATEHPEVPLFTWSLAGNDASLAVEVADTVDNGRLLRCTSAVESF
                     GAVIPFADPASAENAVSVIACCLAENMPSELIARGMADLPAVAMRMETKRGINGCTLI
                     EDYYNSDPASLGMALDFLRTRARGKTTLILSDFRQIGGDENQLYAGVARAVRSSGIDR
                     FIGIGEALSRQRDSFQSGSFFFSSTAEFADAIRGQQFSDETILIKGARVFGLERISGL
                     LVRQLHLTRLEINMNAVVHNLNEFRHLLKPGTGIIAMVKAFAYGSGSAEIASLLEYNG
                     VSRFAVAYADEGVALREAGITSPVMVMNPDESVMDLIIHHNLEPEIYSIGSYSAFAAV
                     ARRHGLLRYPVHIKLDTGMHRLGFGIGDLEELTRQLAGQQHLKVVSLFSHLAASDDPE
                     HDSFTHRQARLLTDAAERLQKALGYRFRLHLLNTSGLVRFPEYQFDMVRLGIGLYGIG
                     RHEGVTLRNTGRFVTAVSQVRTVPAGDPVSYGCRGASDHERQIAVIPVGYADGLRRQL
                     GDGRGSLFIKGRRVPVVGSICMDMCMADVTGMDVKSGDEAEVFGANISIEEIASLCNT
                     IPYEILTSIPQRVKRIYVNE"
     gene            complement(19094..19675)
                     /locus_tag="PKK03_06205"
     CDS             complement(19094..19675)
                     /locus_tag="PKK03_06205"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNX84028.1"
                     /translation="MEFLENSVDHAGKRGSVEVIAGSMFSGKTEELIRRLRRAMFAGL
                     KVEIFKPAVDTRYSEDKVVSHDEKSIMSTPVENASSILLLASGVEVVGIDEAQFFDNS
                     LIEVCTMLADNGTRVIVAGLDMDFSGRPFGPIPALMAVAEYVSKVHAICVRCGNLANY
                     SHRKIKSEKVVVLGEKDIYEPLCRSCYVKAVNR"
     gene            19792..20520
                     /locus_tag="PKK03_06210"
     CDS             19792..20520
                     /locus_tag="PKK03_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84029.1"
                     /translation="MDFLKAVNIIGRDLEEALTLLEQLSDISGSDKAEIELARSRVRS
                     AADMVRLLPSLSGAPDIKTAETPRMAEEPAKPKHEEAVTETSEITQAEVAAPKAGPAV
                     AETAGTGRAEARTPETEPVREEPKNPEQSKTILADRFTSGGTLGEKIQTEKKEEVISS
                     VGHSKPVSDIAAAIGINDRFYFIRELFSGDALAYGDTIKRLNAAASLGEAMRILDEST
                     VMGSDPAAQSSFVDVVRRKFSLHV"
     gene            20513..21211
                     /gene="rsmI"
                     /locus_tag="PKK03_06215"
     CDS             20513..21211
                     /gene="rsmI"
                     /locus_tag="PKK03_06215"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNX84030.1"
                     /translation="MSRLILVPTPIGNLGDITIRALEVLKSAGLVLAEDTRTTRILFD
                     HYGISVPLRSHHKFNEHRSLEAVCDAIEGGTVTALVSDAGTPGISDPGFLLVRRCLER
                     EIPVESLPGPTAFVPALVCSGLPSDRFCFEGFLPQKKGRQKRLSALAGEERTMIFYES
                     PFRILKTLEQLAAALGEDRRASVSREITKKFEETVRGTLASVADHFRTTAPRGEFVIV
                     VAGSSLKDHSEESE"
BASE COUNT         5623 a         5406 c         5052 g         5176 t
ORIGIN      
        1 gccagcaccg aagccggcac atccatcggg ttctgggtga tgaagtaaac tcccacaccc
       61 ttggaacgta taagtttgac tattgtttcg atctgttcga gaaggacctt gctcgcctgg
      121 ctgaaaatca ggtgtgcctc atcaatgaag atcatcagtt ccggcttcgg aacatcaccc
      181 ttctcaggca tggtgttgta tacttcggca agcagcgaaa gcatgaacgt ggagaaaagc
      241 ttcggcttgt cctggatgtc agtaagcctg atgatattta tgtatccgtt cccgtcggag
      301 tctctcctca tcaggtctga aatatcgaat gattcctccc cgaagaaccg ttcggcaccc
      361 tgttgctcca gctctatgat ctttcgcaga attacacctg tggaagcagt tgacatggcc
      421 ccgtattcct tttcaagctc atccttaccc tcaccggtaa ggtactgaat aactttccgg
      481 atatccttaa ggtcaatcaa cgcaagctgg ttgtcatcac agtatttaaa aatgattgac
      541 atgattccgg cctgtacgtc gttgaggtca agtatgcgcg aaaagagaac aggaccgaac
      601 tcggagaccg tggcacgtaa cctgaccccg ttctgttccg agattgtcag cagttccaca
      661 gggaaaccca tggtcgagta cgggaggttg atcttcccgt gcctctccct gatgaaatca
      721 ttttcctgcc ccggcctggc caggccgctg aggtcgccct tcacgtccat cacaagtgaa
      781 ggaattccct tcagcgacaa ctgctcggag atcacctgca gcgtcttggt ttttcctgtt
      841 ccggtggcgc cggcaataag tccgtgacgg ttcagcgtct tcagcgggat cttgatatgc
      901 gcatcggcta tcgcctctcc gtctagcatg gcaccgccaa gaactataaa atcgcccttg
      961 cacgtgtatc cggcgtttat ggaactaaaa aaatcatctt tcctgctcat tgccttaggt
     1021 ttaaagtata taaaagccgt tgagacacct gccccaacgg ctttctaaat tagtaaatat
     1081 ttaaattact tggtatcgag aatttctttt accttctctg caagcgcgtc accgcgaagg
     1141 ttatgcccta cgatgatacc gtttgcatca atcaggaagt tggcggggat ggcatttaca
     1201 gcatactgct tggcaggtgc gcagtcccag tatttcaggt cagaaacatg tgtccaggtc
     1261 agtttgtcat cctggatagc cttcagccag tcttccttgc tgcggtcaag tgatacacca
     1321 aacacgtcaa atcccttctt gttgtactca gcccatacct tgacaacatt ggggttctcc
     1381 tggcggcagg gaccgcacca tgctgcccag aaatcaagca gtaacagttt ggtgtcacct
     1441 ccgattttgg agctgagtga gataggattg ccgtccggat cattgagggt gaaatcaggg
     1501 gccttctgtc ctacagcaac tgccttgagc tgcacaagcc tgtctttcag cgtaatgact
     1561 gtctgaacct tgttgagtgt agtgtcgaat ttgttgagaa tgccttccat ctcatccact
     1621 tcaagataat atgcaagctc gctcaggacc gcgggtgcaa cgaatgatga gggattattt
     1681 gccagaaatt ccttcttgat attggtctgc tttgcatcaa tggcttcaat ttctttttcc
     1741 agtgaggcag caagctcctc attcccgtcc attcttgcct tgcgatagct gtcgtatatc
     1801 tttgtcatct cggcattggt ttcgtcgagc agggccttgt atgaatcata ttctgcctgg
     1861 gtggctgaac cgctcactga tcccatatag agtgaatcag catgtccgtg aacagtaatg
     1921 tctgaattct cgatatagaa acttagaccg cccctcttgc ccctgatgcc aaggttgacc
     1981 atctgcgggt aatcaaccac accttcaatc ttaaaggtgc cattctcaat cgtagctgag
     2041 tcaataacct ggtaaccacc gggaattctt ttctgcagaa ttactgtttc atttccggca
     2101 ccgtcaatgg tacctgtaag agtgaatttg ggttccctgg cgcaggctgc tgcaacaaca
     2161 accaccagca gaaggataac tgtctttttc atctgttgat ttttaattgg taaacacttt
     2221 cttctttcga aggttcaaat atataaataa ttggctactt gcaactgctg tcaaaatatt
     2281 tgtccagcag caatgcagca ggtttatatg aggttacctc cccttcgcga agtttttttt
     2341 ccagttcggg cagcatggca gccacatcag gatttgtata gaatgattgc tttagtgtgt
     2401 caattattgt ttcgtgcatc cttgtcacgg cctgttccct ccttctgtcc cagaaccatc
     2461 cgttgctctt cgtaacactg acataatcac gaatggcttc ccatatatcg gcaatgccgg
     2521 ttctctcaag tgatgagcat gtcatcaccc tgggagtcca cttcgacgga gcaggcggga
     2581 aaaggtgaag tgcgttcttg tattccaccc tcgccatctc ggctttctga atattattcc
     2641 cgtcggcctt ggtgatgacg atagtgtctg ccatctccat gatgcctcgt ttaattccct
     2701 gaagctcatc acccgctccg gcaagcatga gcaccaggaa gaagtcgacc atcgaatggg
     2761 cagctgtctc tgactgcccc acacccaccg tctccacaag aatggtgtca aatccggcag
     2821 cttcacagag aataatgctt tcgcgggtct tgcgtgccac gccgcccaga gtgccggcag
     2881 aaggtgacgg acgaatgaaa gcatcagggt ccgtgctaag ctcctccatc cgggtcttgt
     2941 cgcccatgat gctccccccg ctctgctggc tgctcggatc aatggccaga acggcaagac
     3001 gccttccgta atggcgcgtc agcattgttc caaacgtttc tatgaatgtg ctttttccgg
     3061 caccgggaac acctgtaatg cctatcctga ctgaccttga actggccggg agacaccgct
     3121 ccacaacctc ctgggcaagg tcataatgat ccgtcagatt actctcaaca agggttatgg
     3181 cctggcttag aatggtgcgg ttgccgcgaa ggatgccgtc aacatagtca tcggcactca
     3241 gcatcttgcg cctgtttctc ttcaggcgat tgagcatgtc aggattgata ctcggaggct
     3301 ggggcacccc ctgctgtacc gagagagctc cggctgttct cctttccctg ctcattgcac
     3361 ctctgccccc ggactgacct atttgttaga tgcctcgtcc acaagaacct cccccttgat
     3421 catgagcatc acctcggagt tcttggggtc attggtataa acaaatatat tcttgtagat
     3481 ctggcccttg tagccgcccg aattgaactt cgcctttatc gatccccttg ccccgggctt
     3541 gatgacattc tccgtgggca tgagcgcggt gcacccgcaa ccggctttga catgccttat
     3601 tataagatca cttttgccct ggttggtgaa gaagtactca acatcagccg acgagttctg
     3661 cttcatcttt ccaaagttca cagtgtttga atcaagcttg aaaaccggcg cacttgcaag
     3721 ctgctgggca ttaagccccg aaaaatcctc aacaagattg gctgaaacat acaggtatat
     3781 gttcggctga gactggccat tgacctttag cctgaccagg tcattcacat taccccacgc
     3841 actgctgttg agcttcgcat cgtaggtgcc ctcgattatc cctttctctc ccggcttcag
     3901 agtggcaggt gaaaccttca gctgaagatg ggccggcagc ccttcaaact caaccgtcgc
     3961 aggctccttt gatgtattga tgacaggcat caccctgatc ttcttctctg acttggtggt
     4021 ggtggtgaac gccagatggt tcgactggaa cctcaccggg ccaacggcaa aagtaaaaag
     4081 ctcctcaacc gtcttctccc tgggcgccac attcccctca atcacaagca cggtaacggc
     4141 cggctttgca ttcgaatata cggttacact cttcgcaaaa cggctgctgt aacccttcgg
     4201 gtcgaacaca gcttttacaa atcccttccc tccgggagga acaggtgact tggtatagtc
     4261 ggctgccgta cagccacatg acgactgaac cctcgtaagg acaagcaccg agtctccggt
     4321 attggtgaaa ttgaaaacag cttcctgctt ccccgcctct tccttaatgg ttccgtaatc
     4381 atgacgggtg ttggcaaaat tgagcttagg ctgcgccatc atacctgcac aggccagcat
     4441 caaaaccaga aatgaaatac tccgcttcat atatgcttat gttttttaac ctaaatctgc
     4501 ccgattcatc gtcgattcac tacccttaac aaagatcagg ccagaagggt tgcaccataa
     4561 taaccaccaa aattaacaaa aaagtatgag ccattgtttt tttatttgtt tatagattag
     4621 ttttgtcaca cggatgcacc cgtttggaac agattttgct ccggatgcgg aggccttccg
     4681 ccatttctta tgatacgaaa aggactctat ataccgttcc tgacactctt cttcctcctg
     4741 accgtggcgc cggcttcagg ccagttctat aacggcatgc agatgacctt cggtaagaac
     4801 agggttcagt acaaggactt ctactggacc tacttcagat tcgacgagat tgactgctat
     4861 tacaacgagt tcggcagaga ggtggcggaa tacgcctgca aggtggctta tgaaaaactt
     4921 aatgagattg aggattattt cgactacacc atcgacaagc gccccatact catcatctac
     4981 aacaagaaga acgacttcag gcagagcaac ataggcctta tatcggccga ggatgactac
     5041 aacgtcggcg gctacagcag ggtgatcaag aacaagataa tgctctggtt caacggtgac
     5101 cacgaggagt tcagcaagct tatagcctcg gctatcgccg aagtggtggt ctatgaaatg
     5161 ctctacaacg ctgacctccg cgaccggaca accagcggaa gccagataca gatccctgac
     5221 tggtatatca agggactgat taactacgtc gcctggggat gggacagcga caccgacaat
     5281 cgcgtcaggg acggatttga aaacaaccgc tataaaaacc ttgacaatct tgaatacgag
     5341 gaagctatct atgcgggcca ctcattctgg aaatacatcg gtgacaccta cggcgacgca
     5401 attatcccca acatcatcta cctctcgaag gtctaccgga atatagagga cgggttcctc
     5461 tatggccttg gcaagagcat caaggatacc taccgcgact ggagaaacta ttacagcgct
     5521 tactatgaga cgaagccggg tgagaactac caggttcccc gggaagtgaa aaaggcaaaa
     5581 ccatatgaac ggttcaccga tgtgaaggtg agccccgacg gccgctatgt tgcatgggtc
     5641 tccaatgact ggggcaagag aaggatatgg ctgcatgaca catccaccgg gaagagcagg
     5701 aaaatcttta ctgccgagcc gaaattcgaa cagtcggttg accataccta ccctgtgctt
     5761 acatggcacc ccggcggcca gatactgaca ttcgccaacg aggagaaggg aggcgttcag
     5821 ctttacttct accgtactgc agaaaagaaa ttcgaacaac gaacaatgcc tttcttcgaa
     5881 aaggtactgt cactatccta ctcccctgac ggatcacggc tccttgcatc ggcagtgatc
     5941 aacggcatga ccgatatcta cgtgcataca atcatcagtg gcaccaacga acggataacc
     6001 tttgacattg ccgatgacct gaatcccttg tttgtaaagg gcagtgacga aataattgtc
     6061 ttctcatcca acagggtcac agacacactc accaacaggg gtgacccgca ggagagaaca
     6121 gggcttacat ataatctctt tacatataac ctgggggctg ctgacggcaa actgacaagg
     6181 cttggcgagg attcaatgca ggacctgatg aaacccgtaa tgccgacccc ctcgtcaacc
     6241 ggattcatag ggaacggaaa cgggataatg aaccactaca ccggaaacat tgacagcacc
     6301 atagcgttca ttgacactgc acttcattat acctacttca tcaccccgcg ccaggctacc
     6361 gacttcccga gaaacctcga ggcctttgac agaagcggag gacaggatgc cggtgtcatc
     6421 ttcaaccagg gccggtacag gatctttacc ggaccgtctt ctgccacccc ggtcgctgac
     6481 actgctgaca taaaaacaac cacctggcgc ctggaggaga tcgcaatgct cagggctgca
     6541 gacagcatca accgcttaag ggattggctt ctcgccgaaa gggcaagaat gaatgacacc
     6601 cttaagaaac ctctttacga atactatacc ccggccaacg aaccgataga cttccggaat
     6661 tacgtctttg aaaaggaaaa ggaaaacttc tatgaactgc agtggcggaa agattacatg
     6721 gatatcaacc ttgacacttc aaggctgaag atgcccgatg cacttgtcta ccgccccgca
     6781 ttctacaata actatacggc cacccagatc gacttcaact tcctgaacaa cacctaccag
     6841 gcatataatg gcggagcccc ttacttcaac ccgggactta acatgctatt taaaatagga
     6901 acccttgacc tcttcgagga ttaccgcata acaggaggat tcaggttctc agggaacttc
     6961 gacagcaatg agtacctggt cagtctcgaa gcgctgagag gacggtttga caagcaggta
     7021 atcttccaca agcagtcgtt tgacagttat aatgacacca gttattacaa gataaagagt
     7081 tacactactt acatgtcgct caggaagccg ctgagcgccg ttctggcact gaagggaaca
     7141 ctcacatacc gttatgacag ctatgttgtg caatcgactg accaggccag cctgattgcc
     7201 ccttcgttca accgccactg ggccgggctg aagggcgaag tcatctatga caatacgcgc
     7261 aagcggacta ttaatattta ttacggcacc aggttcaaga ttttcggcga atactacagg
     7321 gaactgactg agaagaagag tgatatgttc gtattggggg ctgacataag gcattaccag
     7381 aagatccacc gtgacctgat ctgggcaaac aggtttgccg ccagtacctc atttggtccc
     7441 tcgaggctta tctattacat gggaggcgtc gacaactgga tgaggcttcc tttcggcaat
     7501 gtgcctcagt ttgaccagac cgtgcctgtc aaccccaatg ttaattatgg tttccaggcc
     7561 ctggccacga atatgagagg ttttacccag aatattcgta atggcagtaa cttcgcactg
     7621 atcaacagtg agatacgctg gccggtggtg aggtatttcg cggggcaccc gctgaggtca
     7681 aacctcctga acagcataca gctcgtcggg ttctatgatg tgggcatggc gtggtcagga
     7741 tgggatccct ggggtaatga aaactattgg aatgacaagg tctaccggaa tggtccggtg
     7801 gtggtaacga ttgatgcaat gcgcgagccg ttggtgatgg gcttcggcgg aggcgccagg
     7861 gcgcagttgt tcggctattt cataagagcg gacctggcct ggggcattga caacggttac
     7921 atactcccca agatcttcta tctgtcgttc agtctcgact tctgataacg accctttggc
     7981 ggaatctttt atcggggcgt aaactgctcc ggttcaggca ttatttattg atgttggcac
     8041 cctatttgat atactcccgg cgagggtgaa ttttatttat gtgtttcgtt aggaaagaat
     8101 gaaaataaga gataatttta cgcccgtaat ctttaaaaaa cagtataatg gcttacaaaa
     8161 tttcagatga ttgcacagct tgcggaacct gtatagatga atgtccggtt gaggctatat
     8221 ctgagggtga catctacaag attgacccgg atatatgtac cgattgtggt gcctgcgccg
     8281 acgtttgccc tgtagaggct atacatccgg cataaccgca ttcccatttt aggaaaattt
     8341 ggaggctgtc ccgaatcaaa aggggcagcc tttctttatg ttgaagatct ggtctccagg
     8401 cacggtaaga ataccggtcg ttctctcacc ggatgacatc agctcacttt atgaagccac
     8461 ctaaataaag aaatggtttg caagggcaga aactaaggag taccaggcag ccatacagca
     8521 gagggaccga gcgatgcttg ggatttatta cggttgtggc ctgagaaaga gtgaaggcac
     8581 taacctgata acaggtgata tattaacgga gaggaaatta atcttcgtga gaaaaggtaa
     8641 aggatgtaag gaaagatatg ttccggtgac cgatggcaac ctcagttaca tcactgaata
     8701 tttacaggat ggccggaact tcttacttgc caggaaccag tccgggacag aatcattttt
     8761 tataaaccag ttcggtggta gttgcagcga tcaggcatta tcggcaagac ttgacaggct
     8821 ggtaaagaat agcgccagca cgggtctgca gggtaagaaa ccaacccttc acacgcttcg
     8881 gcactcaata tcaacgcacc tgttacagtc aggaatggaa atagaaatga tccagaagtt
     8941 cctgggtcat gccagtctcg aaagcaccca gatttataca catattttaa acgaatgttg
     9001 agcgaagcga aatcccgcaa ggcgggaagc tttaaagaat accggcagaa aaaaggctac
     9061 tcagaaaaga gcatccgggt gcaggacagc catgtaaact catttaaatc ctggtgcatc
     9121 agggagaaca taaatcccag tgatatcacc tacaatcaga ccctgaaatt tattgatagc
     9181 gaaaaagaaa gaggtatcag cagacagagc ataataagag aaataaactc gatccggatt
     9241 tactttgatt accttatgga atccgggatg acacatcaga acataattaa acgcatcagg
     9301 ataagacaaa acggaaagaa ggttctcccg gagatcttat ccacgctgca gcttgagaaa
     9361 atataccagg actttagcgg tcttcctgca tggaaccatg gaacagcaac tgcaaagctc
     9421 ttgcaccagc gaacacagtg atccttggct ttttgattta tcaggggctt gacagtggcg
     9481 agatagccag gcttgaaacc aatcacgtta atcttaacga aggcaaaatt tatatccctt
     9541 caggaaggaa aagcagctca agaacactaa agcttcaggc aaaccagatc ttaccgctaa
     9601 aaacctacct ggaagaaacc cgtcctgcca tcctggaaaa acgggagctg caatcaactt
     9661 atctgttccc atcaaagaag tcaagcgacc tggtctgtaa catagtggaa gccgttaaga
     9721 agatccaccc ggagataaaa gacagccggc agctcaggtc aagtgttctt atgaactggc
     9781 tcaaaacaaa taatatacgt caggttcaat acatggccgg gcataagagc atcaggagta
     9841 ctgagagtta tcgaagagag gatcttacgg acctgacaaa gcagcttgag atgttccatc
     9901 cgctcaggtg aaaatggtgg cagcaaaatg tgtcctccaa acttgatatc acaaattgtg
     9961 acctcaagat taaatattta aatttacaaa cctcaaaccc gaaagcatga caaaacaaga
    10021 cactcagata atgatcccgg atgaagtagt aatgagtaaa atatatgtaa tcaggggtgt
    10081 gaatgtaatg atagacaggg acttggccga actatatggg gttgaaacaa aatacctgaa
    10141 acggcaggta aaaagaaata taatacggtt cccggaagac tttatgttcg agcttacaaa
    10201 tcaggaattt aatgaatgga ggagccaatt tgtcacctcc aatgaggata gaatgggact
    10261 tcgttatgct ccatacgcct ttacggagga cggagttgca caactttcca cggtactaaa
    10321 tagtgaaagg gcgattaagg taaatcttca gatcatccga atgttcagta agctccgccg
    10381 gttgacgcta acacacaaag atatattaca caagttggag gagttagaaa ggaatgatat
    10441 cgagcatgac aagaaaatag aactcatttt tgaatatctc aaacaattag aagctgccaa
    10501 acagcaggaa ctggagcaaa agaatcgtaa acgtatcggc ttcaagactc ccggaagcaa
    10561 ggaagactaa tccccgcagc tccccgatgg ccgtgccctg ccggctcgct atcgagaaaa
    10621 cacctgcaac aaccgagcaa ttttaggtag ttcgcttaaa actttggctt aggaaaggta
    10681 gctgccgcct tcccgcagtc aaggttaaaa tgaattttgg aatatttttt tgatagctgt
    10741 tttttatttt caattgaacc ctgaaaggct cacgaacacc tttgaaatta ataacattgt
    10801 gagtaatcaa aaaaatatcc caaaaacctt gacacttccc ctcccgctcg cttcagtctg
    10861 tatctaagcc aaagttaagc catagtgtaa agttttacca gaatcatttc cacaaagaaa
    10921 aaccacaaaa aaagaaagct gaccggaaac catagtgatt ttttctttca attgaaaccg
    10981 ctttcagttg aaaaagaaaa atatgtatct ttagagtaat aacgttcttt gaaaaaacac
    11041 cccgcacctg gtcattgaaa agtcggggca ctggtgagaa aagaggcttt actggtatac
    11101 ttattgcctg aataatcccc tgaagtatac tgatccaagc ggatacctgc ttgatgctga
    11161 atcgtggcaa aggtcgatgt acgaaagcag ttttagaaga tccgggtttt ctcagtcaga
    11221 tatatggatg cttgagaatg acccttactg ggagacctat tttgataatc cctatacctc
    11281 ttcttatgcg acaggttcat catactacag caattattca gcaatttatg ggaaacaacc
    11341 tgagaatcct cacagattta catccggata ctgggtaaga gaatgggttg acagtaatgg
    11401 caatataatc tatcccgacg gactatcagg aatgggatat tttcccgaga ttacgattgt
    11461 agagagattt atacctatgg cgcaggtgga gggggtgatg gaaagagcca gagatccatg
    11521 gtggtacgaa cccgctgatt ttcttacaac ctacctggta agtttagatg tcctcgctag
    11581 attatcgggt ggacttaaag aaatgccagg atgtggacag gttggttttg cagttcaaac
    11641 agtaaagtct ggtcataaaa tattgactgg agataattct atatttcctt acactgattt
    11701 tactgcttca tttattggag cttttgtagg actaggtgga tttattggag ggataaatta
    11761 cttgttaatg gacaagatct gtggcaggga ggaatttgct gaaggcttta taaatgcagt
    11821 aaacctttat agtgaatatg gaaatccaat gtttatccgt tcttggacac actaatttat
    11881 aattagaaaa aggtaaagta aatgggaaat ataattgaat atgcttttaa tagagtatat
    11941 cacatattga gttcactgga tgatgatggt gaaaaatata acaaacatat tacaacatgg
    12001 ttgttttgtt caataattac tgccgttttt attatagata ttgtattatt tttaactaat
    12061 tctaaaaagg tttttttatt gaatgatgga attttctttt tactaatatt tctagttgtt
    12121 gcactttttt attacttttt cactttaagg aaaaggaagt atgtagttat tatagaatca
    12181 tatattaatg aaagcaaaaa gagtatttgg ataggaaata tctgcatctt aatattttca
    12241 ttagctacaa tcgttttatt catcgtaaaa agttctggac tttagggtga tttttgggtc
    12301 cactgcgtag ccaagaaaga aggttgttgt acatggaaac tgttttcttt cctatttcgg
    12361 attgtatttt gcccctgcca gtatagcctg gcaatcggtc attgacatga gctcctccat
    12421 tgtcacatcc ggattgttgt tcatgtaccg ctcgatgatc ctgaaaccgg tccataccac
    12481 tgccctgccg ggcgattcct cggtaaaata acttgtgaac ggagcctctc cggtgaactt
    12541 gcggatcata aagccatcag ttgaaaataa caagtccttg ctcaccagat attcccagat
    12601 cattccctct ccctccttac agaactccat ctgctttcct gtgaacccga aaagaacagt
    12661 gtcagcaagc gcaggcatca tccgcctggt gaaataaacc agcttggcct catgaagcat
    12721 ggagttaaga agggtcttcg tcccgtaccc ggcggagttg taatcccact ccgtggcggc
    12781 ccatgcgtac atacagtcgg aggcagtata atcaggtgtc atcttgcgcg cctggtaact
    12841 gtaaatgcct atggacggat aataggcaca cccggctccc aggtaacggt caaggcttac
    12901 catcagcaga gagtcgtcaa cgatgatact gttgttgaat accgagatac atgttataac
    12961 ctgcggcaca accctgtcag gaaagtagtg tctgtaatgc ctgaatgcgc tctcaagctc
    13021 cttctccagc cgttccgtgt caggccacac cttcttcacc gtatcccaca gatcgaggtt
    13081 gcggagatcg gtggcaaaca gaacgaaagc cgaattccac cgctcgtctg aaggatcacc
    13141 caggccgatg acggtgctgt aagtatcaag tgccctcccg tattcaagct tcagcgtatc
    13201 agccaaggct gccaactcgt tcggaggagt gctgaaaacc tcactcccaa ggtcccttat
    13261 gcgaagatca agctcaatcc ctgacacgtt aaccctgtaa ggatcacggc ggcatgatgc
    13321 tgagaacacg gttaacagca ccagggccgc ggcagtgatt attctggaga aatgagtcat
    13381 gcagtctgta ttgtattgtt gccagtacaa aataaggaca tttatttata attttagccc
    13441 ggctaagact gagaagatga aaagagtaat caaccggacg gtatcaagga caatgattct
    13501 tgtactgatg gctgcgatgc ccgcccgggc cgtcgcgcag gaacttgtat tcgtggtcca
    13561 cgctgacccg gttatctcct ggataggctc caacgagtcg gaatatgcca gtgaaggagc
    13621 acgggcagga ttcgacatag gacttaacgt gctgcattac ttcgccgaga actatgccat
    13681 ctcatcgggc atcagcatca tctccgccgg cgggaggcag agcgctaccg aaaaccatac
    13741 aatggtgttc aataaccttg accccccgac catccccgcc ggcgatgaaa tgaagtataa
    13801 cctgaagtac atgaatatcc cgatagggat ccggcttcag acaaaccagg tggggtacct
    13861 tacctattac actgacctcg ggttcgatgt caggttgctg ctcaaatcaa cggtagacct
    13921 tcccaccctg cagatttttt ctgaaaacgc caaaaacgaa gtcttcggaa tgaatgccgg
    13981 atggcatatc ggaggaggag ttgaatatga actcggcatc gacgcatcac tgattgcagg
    14041 ccttacctac gcgcaggact tctttgacat caccaaggac ctgaaggacg tgaaccagca
    14101 ccctgacagg tcagggctga ggatgttcag gatcaggctc ggactgaagt tctgacatgc
    14161 ccgccatgaa ggttgcactc gcacaactga gctatatccc cggtgacata gcatataaca
    14221 agagtaagat actgaatgcc cttgaagagg cacgtcggca gggagctgac ctggctgtct
    14281 tctccgagct ggcggtaaca ggctaccctc cgctcgacct gctgctgcgc agtgacatca
    14341 tcgactcatc catggccgcc atccgtgaga ttgcaaccca ctgcaacggg atcgccgcca
    14401 ttgtgggagg gccctcaccc aacaccggcg aatacggaaa aagcctttac aactcggcct
    14461 tcttccttca ggagggaaag ataaatgcgg tggtgaacaa agcactgctg cctacatatg
    14521 acatctttga tgaagaccgc tatttcaaag caggaaacac attccggccg gtcgaactca
    14581 acggtcagaa gattgcggtc accatttgtg aagatatctg ggacgagcag ccattcggcg
    14641 acaggggcct ctggcgcctg tacagtgtgg caccactgga tgagataatg aggcaacatc
    14701 cctcactaat cataaacatt gcagccaatc cattctccca taaccgtctc agggtaaggg
    14761 aagaggtatt cgtccgcaac gcaagggaat acagggtgcc gctgatctcc gtcaaccaga
    14821 cgggagggta taccgaactc atctttgacg gttcttcagt tctaattgat tcggacggaa
    14881 ccatcatcga caaactggga ttctgtaccg aggaggtcag gaccgtggag ataccgtcac
    14941 cgggaactca ccggagcagc ctctcattcc ctcccccgct gcctgatgat atgacaggtc
    15001 tcatccaccg tgccctggtc accgggatac gcgatttctt cgataaaggc gggatgaaaa
    15061 aagccataac aggcctttca ggcggaattg actcggcagt ggtgactgca cttgcagctg
    15121 aagccctggg gccggagaac accctcgccc tgctgatgcc ctcagtctat tcatccggac
    15181 attcagtgtc cgactcggtg aagatggcga ctgatcttgg cgtaccttac catatagtga
    15241 gcatcgagga tgcacggcag gcactggaaa aaacacttca gcccttcttc gccggaaagg
    15301 cgcctgatgt taccgaggaa aacatacagt caaggctgcg ggcagttatt ctcatggcct
    15361 tcgccaataa atccgggtat atgcttctca atacctccaa caagagcgaa actgcaaccg
    15421 gctacggcac actttacggt gatatggccg gaggcctctc cgtaataggt gacctttaca
    15481 agaccgaagt gtaccgcctt gccagggaga taaacagcca cgctgagatc ataccccagg
    15541 caataatatc aaaacccccg tcggcagaac tcaagcctga tcagctcgat accgactcac
    15601 ttccacctta cagccagctt gaccccgtcc tctacaggca tatcgacctt gaatggcccg
    15661 ctgaccggat aattgcagac ggatttgata aggagctggt tgagaaggtc atcaggctcg
    15721 tccgcaccag cgaattcaag agaaaacaaa cccctccgat actccgggtc tcctctaaag
    15781 ccttcggtac aggcagaagg ataccaataa tagccgtacg cagctgacac tatcacctga
    15841 cactcaacta tctgaataat agtgtgttaa aaaacgttaa ttttcccttt tgtgtacttt
    15901 tttaatatct ttgtatatgt tgttaaacat aaggggaaat gctttacacc aatccgtacc
    15961 tcgagcaatg catcgaagga ccgttttctg ttttcaggga tcttcctgac aaggagaagg
    16021 agtttctgat acaggatcat tcatgcatta cgctgaaaaa gggggaaatg atttttcacg
    16081 agggtaacag accggcaggg ctttactgcc ttgcatcagg taaggtcaag gtatacaagg
    16141 aaggcatcgg gggacgtgaa cagattatca ggatggtcag gccgcagggc ctgataggtt
    16201 ttgctgcttt tatttccggc aacgcctatt ccgcttcagc ggtggctatc gaagactcgg
    16261 gtatctgccg ttttgacagg gatccgttca tgaagctgat cagacgcaat ccggatctgt
    16321 cgatgaagct catgagaatg atggcagtgg aactctcgtt ctctaacggg agaaccatct
    16381 cgctgaccca gaaacacatc cgtggacgcc ttgccgagtc gctgcttgtc cttcgtgata
    16441 cttacggggt tgagaatgac ggccggacta tcagggccta tgtctcacgc gaagatcttg
    16501 cctgcttttc aaacatgact acctcgaacg ccatacggac tctctctatg ttcgcatccg
    16561 agtctgtcat tgcactggag ggaaaaaaga tcagcattgt tgaccaggca aggcttgaga
    16621 aaataagcca gctgggctga tcactcattc acataaatcc ttttgaccct ctggggtatt
    16681 gaggtgagaa tctcataggg tattgtattg caaagtgagg ctatttcctc aatggatata
    16741 tttgccccga atacctcagc ctcatcaccg gattttacat ccatacccgt gacatcggcc
    16801 atgcacatat ccatgcatat gctgccaacc accgggaccc tccggccctt aatgaaaaga
    16861 ctgcctcttc cgtctcccag ttgcctgcgc agcccgtcgg cgtagcccac cggtatgacc
    16921 gctatctgcc tttcgtggtc tgaggcgccc ctgcagccat agctgaccgg gtcgccggcg
    16981 ggaacggtcc ttacctgtga tacggcagtc acgaaccttc cggtgttccg tagcgtcacc
    17041 ccctcatgac ggcctatacc gtaaagacct attccaagcc tgaccatgtc aaactggtat
    17101 tccggaaaac ggaccagtcc tgaggtgtta agcagatgaa gcctgaaacg gtatccaaga
    17161 gctttctgca gcctctctgc tgcatctgtc agcagcctgg cctgccggtg cgtaaatgag
    17221 tcatgctccg gatcatcact ggccgcaaga tgtgagaaaa gtgaaaccac tttcagatgc
    17281 tgctgcccgg ccagctgcct ggtcagttct tccaggtcac caatgccaaa tccaagcctg
    17341 tgcatccctg tgtcaagctt gatatgtacg gggtaacgga gaaggccgtg tctcctggca
    17401 actgcggcaa aagccgagta agacccgata ctgtaaattt caggttccag gttatgatga
    17461 attatcagat ccatcacact ttcatccgga ttcatgacca tgacaggcga cgtgatgccg
    17521 gcctccctca gtgccacacc ttcatctgca taggcaacgg caaaacggct cacaccgttg
    17581 tactcgagaa gcgaggctat ctcggccgat cctgagccgt aggcgaatgc ctttaccatt
    17641 gcaattatcc cggtgcctgg ttttagcaaa tgcctgaatt cattgaggtt atgaacaacg
    17701 gcgttcatat ttatctccag ccgcgtcagg tgaagctgcc ttacaaggag tccgctgata
    17761 cgctcaagac cgaaaacacg tgctcccttt atcaggattg tctcgtcact gaattgttgc
    17821 ccgcggatgg catctgcaaa ttctgctgtt gaactgaaga agaatgatcc tgactgaaat
    17881 gaatccctct gccgtgacag agcttcgcca atccctataa acctgtcaat cccggaactt
    17941 ctgacagccc tggccacacc cgcataaagc tgattctcat cccctcctat ctgcctgaag
    18001 tcagatagaa taagagtagt ctttcccctt gcgcgggtcc gtagaaaatc cagcgccatc
    18061 ccaagggagg caggatcaga gttataataa tcttctatca gggtgcagcc gtttatgccc
    18121 cgctttgtct ccattcgcat tgccaccgcc ggcagatctg ccatccccct ggcaatcagt
    18181 tctgaaggca tattctcagc caggcagcat gcaatcactg atacagcatt ctccgcagag
    18241 gcaggatcgg caaaaggaat gaccgcccca aatgattcga ctgccgaagt gcacctcagg
    18301 agccggccgt tgtcaacagt atcggctact tcaactgcaa ggctggcatc atttcctgcg
    18361 agtgaccatg taaacagagg aacctcagga tgctctgttg ccatctcctt cctgatgatc
    18421 ctttcatctg cattgcagat cagaagttgt gccccggagg cgagcctgag cttctccgca
    18481 gcaatggctt cccggcctga aaaattttcg ccatgggcgt cacccacatt cgttataatt
    18541 acaacatccg gcatgatgat acgtgccagc ctctccatct ccccgggcct ggaaatcccg
    18601 gcttcgatga cggcaatctc atagctgtca tcaaggttca acagcgagag cgctactcct
    18661 acctgggagt tgtagctttt ggggctgcga atcacactcc gggaagtgct catcaccccg
    18721 gccagccatt ccttgactat tgtctttccc gcgcttcccg ttacagctat gacccttcct
    18781 ctgaagctct cacggcgcca ctttgccatg cactggagtg catacagagt atctgtcacc
    18841 tttacgaaag cggcaccggc catctcacca tggccggagg gaagtgagtc aataagaaac
    18901 agcctcacac ccctggcata caaaggttct atgaatttgt gcccgtcatg attgggcccc
    18961 cgcagggcta tgaaaagcat atctggacca gcgcccggcg acctgctgtc agtcatgacg
    19021 ccggtgacgt tgagattttc atcacctgta agcgttccgc ctgtaatgtg cgccagttcc
    19081 cgggccccga ttatcacctg tttacagcct ttacgtaaca cgagcgacac agcggctcat
    19141 agatatcctt ttcgccaaga acaaccactt tctcgctctt tatcttacga tgggagtaat
    19201 ttgcaaggtt accgcacctg acacatatgg cgtgaacctt gctcacatac tcggcaacgg
    19261 ccatcagcgc aggaatgggc ccgaacggcc ttcccgagaa atccatgtcg aggcctgcca
    19321 caatcacacg cgtgccgttg tctgcaagca tggtacagac ctcgatgagg ctgttgtcaa
    19381 agaactgggc ctcgtctatc ccaaccacct ccacgccgga ggcaaggaga agtatagagg
    19441 atgcattttc cacaggggta gacatgatag atttctcatc gtgtgacaca accttgtcct
    19501 ctgagtatct cgtatctacg gcaggcttga atatctccac cttcagcccg gcaaacatcg
    19561 ccctgcgcaa ccttcttatc agctcctcgg tcttaccgga gaacattgac ccggctatca
    19621 cctccaccga tccgcgctta ccggcatggt caaccgaatt ttcgagaaac tccatacttc
    19681 agttgttatt ggggcgttca atactcagga ttgattaatt cagcccgaaa aactattatt
    19741 gattactttt gcaaaataaa gaaaacggaa ataattaaaa aactaagcga gatggatttc
    19801 ctgaaagctg tcaacattat tggaagggat ctggaggagg cgttgacgtt gctggaacag
    19861 ctctccgaca tatcaggatc agacaaggcg gaaatagagc ttgccaggtc acgcgtgaga
    19921 agcgctgcgg acatggtcag gttgctgcct tcactcagcg gggcgcctga cataaagacg
    19981 gctgagactc cccgcatggc ggaagagccg gcaaagccga agcacgaaga ggctgtgacc
    20041 gaaacttcag agataacaca ggctgaagtc gccgctccaa aggcaggtcc ggctgtggcc
    20101 gaaactgccg gaacaggccg ggctgaggcc cggactcccg aaactgagcc ggtgagggaa
    20161 gaaccgaaga acccggagca gtcgaagact atccttgctg accgcttcac ctcaggcgga
    20221 accctgggtg agaagataca gacagaaaag aaagaagagg tcatctcttc agtcgggcac
    20281 agcaagcctg tctccgatat agctgctgcc attggcatta atgacaggtt ctacttcatc
    20341 cgtgagctct tcagcggtga tgcgctggca tacggtgaca ccatcaaacg actgaatgcg
    20401 gcagcatccc tgggtgaggc aatgagaatc ctcgatgaaa gcaccgtaat gggttccgac
    20461 cctgctgcgc agtcgtcatt cgtcgacgtg gtccgaagaa aattcagctt gcatgtctag
    20521 actgatcctg gtgcccaccc ccatcggcaa tcttggtgat ataaccatcc gcgccctgga
    20581 ggtgctgaaa tcggccggcc ttgtccttgc cgaagacacc aggactacca ggatcctttt
    20641 tgaccactat ggaataagcg tcccgctccg gtcacaccac aagttcaatg agcatcgcag
    20701 cctcgaggcg gtgtgtgacg ccatcgaggg aggtacggtg acagctcttg tctctgacgc
    20761 cggcaccccg ggcatctccg acccgggctt tctgctcgtg cgcaggtgcc tcgagaggga
    20821 gatacccgta gagtcacttc ccggccccac cgccttcgtg cctgcacttg tatgctccgg
    20881 cctgccttca gaccgcttct gcttcgaggg attccttcct cagaaaaagg gaaggcagaa
    20941 aaggctttcg gccctggccg gggaagaacg gacaatgatt ttctacgaat cacctttccg
    21001 tattctcaaa actctcgaac agcttgccgc tgcccttggt gaagaccgaa gggcttccgt
    21061 ctcccgcgag ataacgaaaa aatttgagga gaccgtccgt ggaacccttg cctctgtggc
    21121 agatcacttc cgcacaaccg ctcccagggg tgagtttgta attgtggtgg caggttcctc
    21181 actgaaagac cattccgaag aatcagaata aagatgcgcg gcaggagaca aattccggag
    21241 gcactttctt cattgcc
//
LOCUS       DAOPXU010000045        19905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_2826_length_19905_cov_12.612343, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000045 DAOPXU010000000
VERSION     DAOPXU010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19905
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2826_length_19905_cov_12.612343"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="PKK03_06220"
     CDS             <1..899
                     /locus_tag="PKK03_06220"
                     /inference="COORDINATES: protein motif:HMM:NF021653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNX84031.1"
                     /translation="RDVKSPRLLLGEMNRTTTILRDILSTDFNSIIVDDEVIAGEVKS
                     YIREIAPEKEKIVKVYKEQFPIFDHYGVSRQIKGLFGRTISFKGGSYLIIEHTEALHV
                     VDVNSGNRSKSGSDQESNALSVNLEAAREIARQLRLRDMGGIIVIDFIDMQANDNRQT
                     LHDRMKELMSADRAKHNILPLSKFGLMQITRQRVRPEMTIVTDEKCPVCRGEGKTAPA
                     ILLTDEMERTLSSIIEKVKTTKFILRTHPFVAAYLREGLFSRISRTNKKYGVSIKVQG
                     IESFHFLQYQFCDRQGNEIDLT"
     gene            complement(1048..1824)
                     /locus_tag="PKK03_06225"
     CDS             complement(1048..1824)
                     /locus_tag="PKK03_06225"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HNX84032.1"
                     /translation="MRTLIPALIMAMTTLAACSFFVDCIDGNGDVETEVRDAVPFTAI
                     ANEASFHVNYIQGDEYSVTVEAESNILPYIETEVRGGALEIGTIRGTHCLNYTRHPVI
                     TVTAPVLSEIVNSGSGDFVSGPLSGNDIRIVNSGSGDITAETLAGNVITVIISGSGNV
                     MTDEIDATSLKATLSGSGDLTMSGETVTSRFVVTGSGTVFSRYCSSSTATATLSGSGS
                     VYATVNDQLDAVISGSGNIYLAGDPDINLTRTGSGRVIHL"
     gene            complement(1898..2989)
                     /locus_tag="PKK03_06230"
     CDS             complement(1898..2989)
                     /locus_tag="PKK03_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84033.1"
                     /translation="MNYKTLLITVILLPAGLTLAGQQFSDSKTLRKSFRAGSEAILEV
                     TNKYGDIHVTHTGNDSVTVMVEITASSNTGDKIDALMSDVDVSISQSGSVVRAETTFR
                     KGITPLFESLKGLTKNLVNFNSRLKIDYRIECPPETVLRITNSYGDVYIGDETPVLAL
                     KLSNGNLDAAAVGNAQMMELTFSKANVRSISEGKLTLSFSELRTREAGTIKLASTSSK
                     AWIDGCRAVDLDSKRDDLHFGSVELMTGASYFSDIITDRITGEINMTVKYGNLSFENV
                     DRGFRLTDIRSTYADIDMALEEGSAYDLEIRHTNAFVSLPGFNPEPEKTEINAEDKIF
                     LTKASAGSGAGRSKIRIDATRGEIRLLQK"
     gene            complement(2979..3584)
                     /locus_tag="PKK03_06235"
     CDS             complement(2979..3584)
                     /locus_tag="PKK03_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84034.1"
                     /translation="MDELEKHIRSVRDELDIHEPGPDIWGRIRRDIPGNEEAPETFIG
                     IQEAGGRGRSAKAGIRGRRVQMRSAVWLAAVAVIVAGVAIAIIAGMLLRSQRLNDPQV
                     AAVRETSRYYDERIRTLYEEAEPLLTANPDISTELTLGMNELDSLSAQIIRDLDDNIA
                     SSEVIEALIGNYRLRIELLEDMLSLIKEEGIEAEKNTGNEL"
     gene            complement(3577..4131)
                     /locus_tag="PKK03_06240"
     CDS             complement(3577..4131)
                     /locus_tag="PKK03_06240"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNX84035.1"
                     /translation="MATPDIHIHDRLIDECRGGNRRAQFRLYELYSKAMFNTAHRIMG
                     NREDAEDMLQEAFTDCFRKIGTYRTDSTFGAWLKTIVINRCISRLRKREAELVLIEDY
                     SRHEMQQDEPQEMIWPDPPVIARAVERLPDGYRVVFSLYLLEGYDHTEISQILGISES
                     TSKTQYSRAKEKLKKILSEMRKDG"
     gene            4682..5866
                     /locus_tag="PKK03_06245"
     CDS             4682..5866
                     /locus_tag="PKK03_06245"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNX84036.1"
                     /translation="MTLCLFAVSCKPNEEQISRKAERIHDDILSVDTHCDTPMRFFRG
                     GFDPGVRNDRGCVDFPRMKEGRLDAEFFAIFIGQGPRTPEAFEREHGRTLAIFDSIKA
                     SVAHHQEIAGIALNPDDALRLKTEGKVAIFMGIENGYPIGTDITKIRQYYDLGARYIT
                     LCHSSNNDICDSSSDKGGPEHNGLSAFGEEVVREMNRVGMMVDVSHISDESFYDVLAI
                     TRAPVIASHSSCRALCESPRNLNDDMLQALKKNGGVIQICILSDYLRQPAPNPEYDAK
                     VKELRDRWRNEGQELTEEQQEKRWEEFSKLKEQYVKLATIEDAVNHIQHVIDVIGIDY
                     VGIGSDFDGGGSIEGCTDVSMMKNITKEMIRRGYSRDDIAKIWGGNFMRVMKEVQAAR
                     SI"
     gene            complement(5944..7053)
                     /locus_tag="PKK03_06250"
     CDS             complement(5944..7053)
                     /locus_tag="PKK03_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNX84037.1"
                     /translation="MKGKGYIISGIQQMGVGVYDLREAWEWYIRAFGMDCRIFEDEAE
                     ARLMLPYTGGEPRSRHAVLAMNLQSGGGFEVWQHKGREPEYRDRETKLGDLGIFACKM
                     KVKDVDSAWRYWKANNVEVIGQPLTDPSGRKSFFVKDPFGNLFHIVGATDWFMNLKKI
                     SGGSYGAIIGVSDIDRAMGLYSDILGYDRVVSDTTGTFDDLAPVPGGSGRFRRMLLAP
                     SRPFHGGFNPIFGQSVIELVQALDREPTRIFEGRLWGDPGFIHLCYDISGMDNLRKYC
                     SEAGFPFTVDSKASHEGNSFDMGEAAGFFAYIEDPDGTLIEFVETHKVPILKKFGWYL
                     DMRRRDPNKPLPSWMIKSLRFSRVKGKNKAVGSRQ"
     gene            complement(7064..7864)
                     /locus_tag="PKK03_06255"
     CDS             complement(7064..7864)
                     /locus_tag="PKK03_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX84038.1"
                     /translation="MKDIFTGKVVWITGASSGIGEALAKAFARGGSRVIASSNDTAGL
                     ERVAGECSGMKGSITCVPFDLSDTSGIESLVKRVAQGEGRIDMLLNIGGISQRALLIE
                     TPIWLDRKIMEINYFGTIALTKAVLPHMIAGGGGHIAATSSITGRFGFPLRSAYSASK
                     QALHGFFETLLIEHGKDNIRVSVLIPGRVQTAISLHALTAAGKEHGRMDDGQKGGVTP
                     ERAAEQIIRGLQRNKREILVGSRELLMLKIRKYLPSLFFRIAGRIKPM"
     gene            complement(7867..9195)
                     /locus_tag="PKK03_06260"
     CDS             complement(7867..9195)
                     /locus_tag="PKK03_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNX84039.1"
                     /translation="MGKKNIEKYDIFYQLLKKYVDFWHNYIFYRRVIVLGKDTVDFSV
                     PTILAPNHQNALMDAMAVLCTLDRQLVFLARVDIFRKKFVAKILYFLKMLPVYRLRDG
                     FDAVKQNDDTFRDTLRVMENRNGVVILPEGNHATFRKLRQLKKGICRIAFQTAEAREF
                     KGEINLVPVGLEYSHYWRFRQVLTVVYGHPIHVSEYYDSYRENPNRAIAELRDRLSDE
                     MKKVMVHIEDVENYEAVNELRSIINEKYSDKIKYPKLFRDQRLVSKLNELRLYDQESY
                     REICEGSLSVRDNATALNVTYRHLRKKSHRLLWLTLSSLLLLVTIPVFVAGALFNLLT
                     YQVAYQQAIKSKDPAFISTARYGFFLGLSFIFGPLYLVLALILIKPWWLAIMAFMIIP
                     LLGILAWNWFLLLLRTIGGFRIWNLRRTGNPVFSTLLDTYNKLVKRVSSL"
     gene            complement(9394..9771)
                     /locus_tag="PKK03_06265"
     CDS             complement(9394..9771)
                     /locus_tag="PKK03_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HNX84040.1"
                     /translation="MTAEKGERIDKFLWFVRLYKSRSIATEECRKGRVIVSGHPAKPA
                     SLIAPGATIIVRKPPVTYTYTVIGIPGGRIGAPLVSSYITDITPEEEKTKLIPGRMVS
                     GYRPRGSGRPTKRERRELDDFLE"
     gene            complement(9845..10453)
                     /gene="pth"
                     /locus_tag="PKK03_06270"
     CDS             complement(9845..10453)
                     /gene="pth"
                     /locus_tag="PKK03_06270"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNX84041.1"
                     /translation="MKHLIAGLGNIGDEYRNTRHNVGFMVLDAAVAASGIVFKDSRYG
                     YVARMRHKNAELVLLKPSTFMNRSGNAVRYWMQKEKIEPENLLVITDDLAIPTGSIRL
                     RSAGSAGGHNGLTSISEVLGTDQYARLRIGIGSDFPRGRQVDYVLGEWSPEELIIMEK
                     RIPLAVEMMLSFAVAGCELTMTAYNRKGKNPGDDSAPPAARQ"
     gene            complement(10453..11091)
                     /locus_tag="PKK03_06275"
     CDS             complement(10453..11091)
                     /locus_tag="PKK03_06275"
                     /inference="COORDINATES: protein motif:HMM:NF004132.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HNX84042.1"
                     /translation="MKTIEIKAFPREHFGKKSTNSLRAEDNVPCVMYMEKENLHFYAH
                     ENAFRGLVYTPDVFLVNLEVAGKSYKAVMKEIQFHPVSDRLQHIDFMQVSEDKPVTID
                     IPLKITGESSGVKAGGKLRVKRRTLKVKGLTKYIPDHMTIDITGLGIGQSVKIGDLNY
                     ENLDIIDNKRAMIVAVEVSRVSLKEEEAAAPGAEAPAAEAPAPAKEQPGKGA"
     gene            complement(11197..12135)
                     /locus_tag="PKK03_06280"
     CDS             complement(11197..12135)
                     /locus_tag="PKK03_06280"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNX84043.1"
                     /translation="MIGRAPFKIFSCTSSLTIAEKIADHLGIELGKSSLLSFSDGEFQ
                     PCFDESVRGCHVFIIQSTNPPAENLMELLLMIDAAKRASAYKVNAVIPYYGYARQDRK
                     DRPRVSLGAKLTADLLTKAGVDRIITMDLHADQIQGFFDVPVDHLFGSALFAPYIRNL
                     NLPSLTVSAPDMGGSKRANAYARYLQCEMVLCYKLRKKPNVVEEISIIGEVEGRHVVI
                     IDDMVDTAGTLTFAATVMRDRGALSVRAVATHPVLSGNAVEKIDKSPLEELVVTDSIP
                     LICQSPKIKVLSIGEIFAEAIKAVYTNTSISSGFVF"
     gene            complement(12196..12804)
                     /gene="yihA"
                     /locus_tag="PKK03_06285"
     CDS             complement(12196..12804)
                     /gene="yihA"
                     /locus_tag="PKK03_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNX84044.1"
                     /translation="MIIRSASFVTSSARISQLPPPDRPEYAFIGRSNVGKSSLINMLV
                     SKKGLAKTSGSPGKTQTINHFVINESWYLVDLPGYGYARVSQKMRDQWDRLFTSYVTM
                     RQNLILLFALIDGRHEPMASDLAFMERLALNGVPFARVFTKTDKLSAAEAAKIRDVHD
                     RVMLRSWESLPPTFVTSASRGTGREEVLSYIESSMIFFTGMK"
     gene            complement(12804..13190)
                     /locus_tag="PKK03_06290"
     CDS             complement(12804..13190)
                     /locus_tag="PKK03_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNX84045.1"
                     /translation="MSQILVPGIRLTTTRTVSTNDTAAVYGSGLHDVLSTPAMIAFME
                     QTAMKAVEACLDEGEGTVGTEVNIRHLKATPVGKTITCTATLQEVNGNRLLFHVEADD
                     ETGKIGEGKHERFIIDNRRFKEKLNG"
     gene            complement(13196..13957)
                     /locus_tag="PKK03_06295"
     CDS             complement(13196..13957)
                     /locus_tag="PKK03_06295"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84046.1"
                     /translation="MKQFTLLTAAIIMILASACGGGAGQEGSGRKKDVQVADTGYTGI
                     KNYVRDNVKLREVTYKNGIREGMTRTFYKGGQLEQEIPYSGDSKNGEAKWFYPDGKVF
                     RVTPYVNDTIHGDQVQYYKNGGVKARLRYTDGRRHPGLEEFLMSGEKVTGYPAIRYRV
                     NDRYAERGLYKIFIEFDDMAENVKFYRGDYVNGLVDLDSLSPLLQTATTGYLDLQKKP
                     GHHADSVAVIGSYLTQFGNRLYYRQAIRLPYKDLN"
     gene            complement(13954..14952)
                     /locus_tag="PKK03_06300"
     CDS             complement(13954..14952)
                     /locus_tag="PKK03_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HNX84047.1"
                     /translation="MSSLYPIRFKPMLREMLWGGDTLRERFGKKAKPGARIGESWEIC
                     GMPGKSSLVANGFLKGNSLEEIAEIYMDELLGEAVYAKFGTEFPLLVKFIDAADKLSI
                     QVHPDDRLASERHHAWGKTEMWYVIDTKPGGVIYTGFRKKTTREEYLEYLGSKKLIDL
                     INVTPVRTGDVFFIPAGLVHAIGGGVLLTEIQQTSDVTYRIYDWDRVDGSGRPREMHT
                     SLALDAINFSFDSTNLIRTVPELNRTVLLAESQYFHTSLITFDRPIIKDYSLTDSFII
                     YICTQSMAVVECNGHSEEIKAGETILIPASADSVAIIPRGTATLLEVFVPQKTTSK"
     gene            complement(14958..15260)
                     /locus_tag="PKK03_06305"
     CDS             complement(14958..15260)
                     /locus_tag="PKK03_06305"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HNX84048.1"
                     /translation="MVKHIVIFKLTSPVTPEERNQSVMKLKEIFGPLGNRLKYVIEYR
                     TGVNITDVDHAGDFVIDATFATLDDLRRYQSSEPHREAVAAASIIKKAKLVVDYTF"
     gene            15302..15910
                     /gene="udk"
                     /locus_tag="PKK03_06310"
     CDS             15302..15910
                     /gene="udk"
                     /locus_tag="PKK03_06310"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNX84049.1"
                     /translation="MLIVGIAGGTGSGKTTVVRKLIEMLPPGEVVILPQDSYYRDNSH
                     IPVEQRQNINFDHPDSVEWPLLIAHLAELKAGRPIRMPVYSYLTCVRSEETVPIRPAE
                     VVVVEGILVLADPGLRQMLDIKVFVDADADDRLGRVIQRDIIERGRSVLMVLERYNKT
                     VKPSHLQFIEPSKRYADIIIPGGGENLVGIEVLKTIVEKHLR"
     gene            complement(15956..16675)
                     /locus_tag="PKK03_06315"
     CDS             complement(15956..16675)
                     /locus_tag="PKK03_06315"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNX84050.1"
                     /translation="MTRLSVNINKVATLRNARGGNVPDVKLAAVKCQAFGAEGITVHP
                     RPDERHIRYSDVYDIRDIVTTEFNIEGNPEEQFIRLVLDVVPDQVTLVPDAHDAITSN
                     AGWDTRKNLSFLTDVIARFHGKGIRTSIFTGTDPEIIEYAARTGTDRIELYTEPYASG
                     YHADREAAVAPFVTAAIAARDAGLGINAGHDLDLHNLAWLHSHIPWLMEVSIGHALIS
                     DALYYGLENTIQMYLRELRKS"
     gene            16756..17421
                     /locus_tag="PKK03_06320"
     CDS             16756..17421
                     /locus_tag="PKK03_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNX84051.1"
                     /translation="MLAKDMISDVVPALKTSDTAQTALNWMEIFRVSHLPIVNESDYL
                     GLISDSDIYDLNQPEEPIGNHPLSLPRPSVKIHQHIYEVLALAARHKLTVVPVIDDDN
                     HYRGVITCNEIISGIASISAIDQPGGILILKVLQRDYSMSRISQIVESNDARILSMHI
                     TSDPDSTSLEITLKVNTTDLVSIIKTFERYSYEVTTWVTDNDELDRFYTDRYNLLMKY
                     LNM"
     gene            17434..18312
                     /locus_tag="PKK03_06325"
     CDS             17434..18312
                     /locus_tag="PKK03_06325"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNX84052.1"
                     /translation="MDKTIALYSKSLTEKTTAGLMRVIDALYDRGVTIYLNIMDPEGH
                     HFNHDHRVILFSELSDLPAYVSMILSVGGDGTFLETAMRVRTAGIPVAGINTGRLGFL
                     ANIPDNEIGKSVDLIISGDYEVISRSMLEITSPPELAADGNAMALNEITVQKDDQSMI
                     TITVEVDGTFLNTYRADGLIISTATGSTAYNLSVGGPILYPTDDSIIIVPMSPHNLTV
                     RPIIVSGSSVITLGTGGRSSHCLVTIDSKATRVPSGTVLEVRQSASRLSTVTIAGNDF
                     FSTLRNKLMWGADPRN"
     gene            18387..19181
                     /locus_tag="PKK03_06330"
     CDS             18387..19181
                     /locus_tag="PKK03_06330"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNX84053.1"
                     /translation="MVKRLIVMCMVVSFTAPFVSGQLWKMKRYEATAAIGTSQFYGDI
                     GGYTIGENALGFKDITFIQTRYSVNGSFRYFITDNFSARLSLTWAALHADDRRGSNND
                     GRLYETSSFLYEPALLGEYYFVRNRERNSFLFQTYSYRRANRLRDFFRSVDVYALTGI
                     GGAGYNVFYGNDKIQEEKWDLDPTLRRSGFTAVIPLGVGAKLAIDPNLLVGVELAGRY
                     ALSDYLDGYTSVYSKRNDAYHTFSVTVSYRIKTARNGLPDFRTLRQ"
     gene            19190..19837
                     /locus_tag="PKK03_06335"
     CDS             19190..19837
                     /locus_tag="PKK03_06335"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNX84054.1"
                     /translation="MKRSIIITILIILALPALPQRTADYGVSAGVVNYLGEQNPLSAF
                     FKPGPAVQLFYRYNLNPRQAVRANLLGGYMTPLGSFLGELGATYEFNFFPYSTFRSRH
                     IDYSPYIAGGAALSMMSQAGPFLSVPFSAGFKVNVANQVGVEIEYGFRKTFTDKLDGQ
                     FEITDSGGEPVDRTWTHNNDWYSFLGVSVTWKIYNKLAGCPAFAEMGKDKNRKRR"
BASE COUNT         4621 a         5296 c         4957 g         5031 t
ORIGIN      
        1 accgcgacgt caagtcgccg cgactgctcc tgggcgagat gaaccgcacg acaacaatcc
       61 tccgggatat tctcagtacc gacttcaaca gcatcatcgt tgatgatgag gtgatagccg
      121 gcgaagtgaa atcttacatc agggaaatag cccctgagaa ggaaaagata gttaaggtat
      181 acaaggagca attccccatc tttgaccatt atggcgtcag ccgtcagatc aaggggctct
      241 tcggacgaac catctctttc aagggcggtt cataccttat catcgagcat actgaagcac
      301 tgcacgtcgt tgacgtcaac agcggcaaca gatcaaaatc aggctccgac caggagtcaa
      361 atgccctttc tgtcaatctt gaggctgcaa gggaaatagc ccgtcagctt cgcctgaggg
      421 acatgggtgg aataatcgtg atcgacttca tcgacatgca ggccaacgat aaccgccaga
      481 ccctccatga caggatgaag gaactgatga gcgctgacag ggcaaaacat aatattctgc
      541 ccctcagtaa attcggactg atgcagataa caaggcaaag ggtgcgcccc gaaatgacca
      601 tcgtcactga cgagaaatgc cctgtctgca ggggagaggg gaaaacagca ccggctatac
      661 tccttaccga tgagatggaa cggacactct cttctatcat cgagaaggtg aagaccacaa
      721 aattcatcct caggactcat cctttcgtgg ctgcctacct gcgtgaaggg ctcttctcca
      781 ggatcagcag gacaaacaag aagtacggtg tgagcattaa ggttcaggga atcgaatcat
      841 tccatttcct gcagtatcaa ttctgtgaca ggcagggtaa cgaaatcgac cttacctgat
      901 tttcctgtta tacatcaaat tatgaataca tattagtaac ggcaggtctc agacctgccg
      961 ttacgatatg gttgccatgc atttccgtac gggagatgga gcggcaggtc tgagacctgc
     1021 cgttacgtgc ttgtatgcac atattgttta taaatggatg acccggcccg aacccgtgcg
     1081 ggtgaggttg atatccgggt cacctgcgag atagatgttg ccgctgccgc tgataaccgc
     1141 atcaagctga tcattgaccg tggcatagac tgatcctgat cctgacaatg tggctgtggc
     1201 tgtgctgctg gagcaatagc gtgagaaaac agtgcctgac cctgtgacaa caaagcggct
     1261 cgttaccgtt tcacctgaca ttgtcaggtc acctgaccct gacagggtgg ccttcagtga
     1321 ggtggcatcg atctcatccg tcataacgtt tcctgatcct gatatgatta ctgtgatcac
     1381 gttgcctgca agggtttcgg cagtgatatc acccgaaccg ctgttgacaa tccttatatc
     1441 attgcctgac agcggccccg aaacaaagtc gcctgatcct gagttgacga tctcgcttaa
     1501 caccggagca gtgaccgtga tgaccgggtg ccttgtgtag ttaaggcagt gggtgcccct
     1561 gatggtacct atctccagcg caccacccct tacttctgtc tctatataag gcagaatatt
     1621 gctttcggcc tcaactgtga cagagtattc atcaccctgg atgtaattca cgtgaaatga
     1681 tgcctcgttg gctattgccg taaatggtac tgcatccctc acttcggttt ccacatcccc
     1741 gttgccgtca atgcagtcga caaagaaact gcaggcggcc agtgttgtca tggccattat
     1801 taaggccgga attagtgttc tcatattcat tttccttttt actgttcccc cttaaggaca
     1861 taaaaaaatc gggtcagttg catttctgcc acaaaaacta cttctgcagc agccttattt
     1921 cgcccctggt ggcatcaatc ctgatctttg accggccggc accgctgcca gccgaggcct
     1981 ttgtcagaaa aatcttgtct tcagcattta tctccgtctt ctccggttcc gggttgaatc
     2041 ccggaaggga gacgaatgca ttggtatgcc ttatctccag gtcataggct gaaccttctt
     2101 ccagggccat gtcaatgtca gcataggtcg agcggatgtc tgtcagcctg aatccacggt
     2161 caacattctc aaacgagagg ttcccgtatt tcactgtcat atttatctcc ccggtaatcc
     2221 tgtcagttat gatgtcgctg aagtagctgg cccccgtcat taactccacg gagccgaaat
     2281 gaaggtcatc acgcttggag tcgaggtcaa ccgcacggca gccgtcaatc catgccttgg
     2341 atgatgtgga ggcgagcttg atcgtccctg cctcccgggt gcgcagttcg ctgaatgaga
     2401 gggtcagctt cccctcgctg atgcttctta cgttggcttt gctgaaggtg agttccatca
     2461 tctgtgcatt acccacggcg gcagcatcca ggttgccgtt tgaaagctta agtgccagaa
     2521 ccggtgtctc atccccgatg tatacatcac cgtaactgtt ggtgatcctc agcacagttt
     2581 ccggcggaca ctctatgcga tagtctatct tgagacgtga gttgaagttt acaaggttct
     2641 ttgtaagtcc cttgagactc tcaaagaggg gtgtgatccc cttgcggaat gtagtttccg
     2701 cccgtacaac cgaacctgat tgcgaaatac tcacatccac gtctgacata agtgcatcaa
     2761 tcttgtcacc ggtgtttgac gaggcggtga tctcaaccat gacagtgaca gagtcattgc
     2821 cagtatgggt gacatgtatg tcgccatact tgttggtcac ctcgaggata gcctcgctgc
     2881 ctgccctgaa ggatttcctc agggtcttgc tgtcagaaaa ctgctggccc gcgagtgtaa
     2941 gtccggccgg cagcagtatg accgttatta acagagtttt atagttcatt gcctgtgttt
     3001 ttttcagctt caatgccttc ctccttaatg aggctgagca tgtcttcaag gagctctatc
     3061 cggagcctgt agttgcctat cagggcttca atcacctcgc tgctggcaat attgtcatcc
     3121 aggtcacgga tgatctgtgc cgagaggctg tcaagctcat tcattcccag ggtcagttcg
     3181 gtgctgatat ccgggttggc tgtcagcaga ggttctgctt cctcgtaaag tgttcttatc
     3241 ctttcgtcat aatacctgga ggtctctctt acggctgcca cctgcggatc attcagcctc
     3301 tgggacctga gcagcatgcc tgcaatgatg gcgatggcca ctccggcaac tatcactgcc
     3361 actgcggcaa gccagaccgc actgcgcatc tgcacccgcc tgccacgaat ccctgccttg
     3421 gcgcttcggc ctctgcctcc ggcctcctgt atgccaatga aggtctcagg cgcctcctcg
     3481 ttaccgggaa tgtcacgcct gatcctgccc caaatgtcag ggccgggctc atgtatgtca
     3541 agctcatccc tgacgctcct tatatgtttc tcaagttcat ccatcttttc gcatttctga
     3601 gagtatcttt ttaagttttt cctttgccct cgaatactgt gtttttgagg ttgattccga
     3661 gatccccagt atctgtgaaa tctcagtgtg atcatatcct tcaagcaggt agagactgaa
     3721 aaccaccctg tacccgtcag gcagccgttc aacagccctg gcaatcactg gcggatcagg
     3781 ccatatcatc tcctgtggct catcctgctg catttcatgc cggctgtagt cctcaatgag
     3841 caccagctca gcctccctct tccggagcct gctgatacac cggtttatga ctattgtctt
     3901 cagccatgcc ccgaaggtgg aatcagtgcg gtatgtccct atcttcctga aacagtccgt
     3961 aaacgcttcc tgcagcatat cctcggcatc ctccctgttg cccattatcc tgtgggcggt
     4021 gttgaacatc gccttcgagt aaagctcata cagcctgaac tgtgctctcc ggtttccccc
     4081 gcggcactca tcaatgagcc tgtcatgtat gtgtatatcc ggtgtggcca agttccaggt
     4141 tgttcctgat caatgacgca tcaaatttac actagttgca tcaacagtgc agaatccatt
     4201 ttccggtctt cttttttttt caaatattag gaacaccaac acattttaat tatatttgcc
     4261 tttagtaaat aacctgatca gacaagtaca aaatacagcg gaagaaattt aagatgcttt
     4321 ttactgtcca aaagcaattt ttccattctt ttcatggctg ccccgacact tagtcggggc
     4381 tttttttgtg gctccggtgg tccgtgggct ccagctccag ggttccggtt gtccgctgac
     4441 tcataaatgc ctgcccccgg attcattggc atgatttgcg taaaaaacct gtgattcacc
     4501 ccttttagtt cagaaaagcc cttcggctga ctgcctgcag acacccccgc tctcattcat
     4561 actcatacat ttaacctttt ttatgacttt agcagccggc cggcagtcat aatcagcctt
     4621 tgatatttat atcttagcac tgacatctta aattaatacc atgcgtacta aattcctttt
     4681 aatgacacta tgtcttttcg cggtgtcatg caagcccaac gaagagcaga tcagccgcaa
     4741 ggctgaaaga attcatgatg acatcctttc cgttgacaca cactgtgata cacccatgcg
     4801 gttcttcagg ggcggttttg accccggagt gcgcaatgac aggggttgtg tcgattttcc
     4861 aaggatgaag gagggaaggc ttgatgctga attctttgcc attttcatcg gtcaggggcc
     4921 gcgtaccccc gaggctttcg agagggagca cggaaggacc cttgcgatat tcgactcaat
     4981 caaggcaagc gtagcccacc accaggaaat agccggaata gcacttaacc ctgatgatgc
     5041 cctgcggctt aagactgaag gcaaggtggc gatattcatg ggaatagaaa atggctatcc
     5101 gataggtaca gacatcacaa agatcaggca gtactatgac ctcggtgcac gctatatcac
     5161 cctgtgccat tcttccaaca acgatatctg cgactcatca agtgacaagg gcggtcctga
     5221 acataatggc ctttccgcct tcggcgaaga ggtggtcagg gaaatgaacc gcgtcgggat
     5281 gatggttgac gtgtcacaca tctctgacga atcgttttat gatgtactgg ccataacgag
     5341 ggcgccggtg atagcctctc actcatcgtg ccgcgcactg tgcgagagtc cgcgaaacct
     5401 gaatgatgac atgcttcagg ccctgaagaa aaacggaggc gttattcaga tctgcatact
     5461 gagcgattat ctcaggcagc cggcaccaaa cccggagtat gatgcaaagg tcaaggaact
     5521 gcgcgacagg tggaggaacg agggtcagga gctgacagag gagcagcagg aaaagcgctg
     5581 ggaggagttc tcaaagctga aggaacagta tgtcaaactg gctaccattg aagatgccgt
     5641 gaaccatatc cagcatgtga ttgacgtcat cggtatcgac tatgtgggca taggatctga
     5701 tttcgacggc ggaggcagca tagaggggtg cactgatgtc tccatgatga agaacatcac
     5761 gaaggaaatg atccgccgcg gctacagccg tgatgatatt gccaagatct ggggcgggaa
     5821 cttcatgagg gtcatgaagg aagtgcaggc agcaaggagt atttagcagg caataggcaa
     5881 taggcaatag tgttgacatc atcgttaccg aaactgcctg gtatccagta ttttcactac
     5941 tgcctattgc ctgctgccta ctgccttgtt tttgcctttg acccgtgaga acctcaatga
     6001 tttgatcatc catgacggca gtggcttgtt ggggtcacgg cgccgcatgt caagatacca
     6061 tccaaacttt ttgaggatgg gcaccttgtg cgtctccacg aattcgataa gggtgccgtc
     6121 aggatcttcg atataggcga agaagcctgc tgcctcgccc atgtcaaagc tgttcccctc
     6181 atgactcgcc ttgctgtcga ctgtaaacgg gaagcccgcc tcgctgcagt atttgcgcag
     6241 gttatccatc ccgctgatgt catagcagag gtgaatgaac ccggggtcac cccagagtct
     6301 cccctcgaat atcctcgtcg gttcgcggtc aagcgcctgt acaagctcaa tgacactctg
     6361 gccgaagatg ggattgaatc cgccatggaa tggtcttgag ggtgcgagga gcatgcgcct
     6421 gaagcgaccg ctgccaccgg gtaccggagc caggtcgtcg aaggtgcctg tggtgtcgga
     6481 caccaccctg tcatatccga gtatgtcaga ataaagcccc atagcgcggt caatgtccga
     6541 tacgccgata atggcgccgt atgatccgcc ggatatcttt ttcaggttca tgaaccagtc
     6601 ggtggctccc acaatgtgga acaggttgcc gaacgggtct ttgacgaaga agctcttcct
     6661 gcctgaaggg tcagtcagcg gttggccaat cacctccaca ttgttggctt tccagtatct
     6721 ccaggcgctg tcgacatcct tcaccttcat cttgcaggcg aagattccca ggtcgcccag
     6781 tttggtctcg cggtcacggt attccggttc tcttcccttg tgctgccaca cctcgaatcc
     6841 gccgccactc tgcaggttca tcgccagcac ggcatgcctg cttcgcggct cgccgccggt
     6901 atatggcagc ataagcctcg cctccgcctc atcctcgaag atgcggcaat ccatcccgaa
     6961 cgccctgatg taccactccc atgcttctct gaggtcataa acccctacac ccatctgctg
     7021 tattccactg ataatgtaac ctttgccttt catgactcct tccttacatt ggtttgatcc
     7081 gccccgctat cctgaagaaa agcgatggta aatatttcct tattttaagc atcagcagtt
     7141 ccctgctgcc gacaagtatc tcccttttgt ttcgctgaag ccctctgatg atctgctctg
     7201 cggccctttc tggggtgacg ccgcctttct gcccgtcgtc cattctgccg tgttcctttc
     7261 cggcggctgt gagcgcatga agcgagatgg cagtctgtac ccttccgggt atgagcacgg
     7321 agacccggat gttgtccttg ccgtgttcaa tgagaagagt ctcgaagaag ccgtgcagcg
     7381 cctgcttgga ggctgagtat gctgagcgca gcgggaaacc gaaccggcct gtgatggagc
     7441 tggtggcagc aatatgcccg cctccgcccg cgatcatgtg aggcagcact gccttggtga
     7501 gagcgatggt gccgaaatag ttgatctcca tgattttacg gtcaagccag atcggtgtct
     7561 ctatcagcag ggccctctgg cttatccccc cgatgttgag aagcatgtca atccggccct
     7621 caccctgagc caccctttta accaggcttt cgatgccgga ggtatctgaa aggtcaaaag
     7681 ggacgcaggt gatactcccc ttcattccgg agcactctcc ggctaccctt tccagccctg
     7741 ccgtatcatt agatgatgct atcaccctgc ttccgccccg ggcgaaggcc tttgccaatg
     7801 cctccccaat tcctgaggag gccccggtga tccagaccac ctttccggta aaaatatctt
     7861 tcattttcag agagaagaga cgcgtttaac gagtttgttg tatgtgtcga gcaatgtgct
     7921 gaagaccggg tttccggtgc gacggaggtt ccaaatcctg aaaccgccta tggtgcgaag
     7981 cagcagcaga aaccagttcc atgccaatat gcccagcaac gggatgatca tgaacgccat
     8041 tattgccagc caccacggct tgatgagaat aagggccagt accaggtaga gcggtccgaa
     8101 aatgaagctc agccccagga agaatccata ccttgcggtg ctgatgaatg cgggatcctt
     8161 tgacttgatg gcctgctggt atgccacctg gtaagtcagt aaattgaaca gggcgccggc
     8221 gacaaatact gggatggtga ccagcagcag cagtgatgac agtgtcagcc ataatagccg
     8281 gtgacttttc ttcctcaggt gacggtacgt gacattcagc gccgtggcgt tgtcacgcac
     8341 cgaaagagat ccctcacata tctccctgta gctctcctgg tcatacagtc tcagctcgtt
     8401 gagcttgctg accaaccgct ggtcacggaa aagcttcggg tactttatct tgtcactgta
     8461 cttttcgtta atgatgcttc gaagttcatt gacagcctca tagttctcca catcctcaat
     8521 atggaccatc accttcttca tctcatccga gagcctgtca cgaagttcag ctatagccct
     8581 gttgggattc tcacggtacg agtcatagta ctccgataca tggatcggat gcccgtacac
     8641 aacagtaagc acctgcctga aacgccagta atgtgagtat tcaagtccga ccggaaccag
     8701 gttgatctct cccttgaact ccctcgcctc agcggtctgg aaggcgatcc ggcagatacc
     8761 cttcttcagc tgtctcagtt tcctgaaggt ggcatgattg ccttcgggca ggataaccac
     8821 tccgttgcgg ttttccatca ctctcagtgt gtcacggaag gtgtcatcat tctgcttcac
     8881 ggcatcgaaa ccgtccctga ggcggtagac gggaagcatc ttgaggaaat acaggatttt
     8941 tgccacgaac ttcttcctga atatatcaac cctcgccagg aacaccagct gacggtcaag
     9001 ggtgcatagc acagccatgg catccatcag tgcattctgg tgattgggcg ccagaatagt
     9061 gggcacggag aaatcgacag tatccttgcc caggacaatt accctgcgat agaaaatata
     9121 attgtgccag aaatcgacat acttctttaa aagctgataa aaaatatcat acttctcaat
     9181 gttcttctta cccattccgc aacaaggttt ttaccttctg ttaagagtcg tgaagcccgc
     9241 gggcctcaca atttacgaag ttatgaaaag tttatccgca gactgaataa aatggcagga
     9301 gtaagggtgc atcacctctt ttttaggtat ttataccacc atgaccgggg ctgcgcggtt
     9361 caacccatgg attaattcgg gaggactttg attctattcc aggaagtcgt caagttccct
     9421 gcgttccctc ttggtcggac ggccgctgcc gcgaggccgg taaccgctga ccattcttcc
     9481 cgggataagc tttgtcttct cttcttccgg ggtgatgtca gtgatgtagc tgcttacaag
     9541 cggcgcaccg atccttcccc ctgggatgcc gataacggtg taagtgtaag tgacaggcgg
     9601 tttcctgact atgatggttg cgcccggggc gatgagcgat gccggtttgg caggatgacc
     9661 gctgacgatg acccttccct tcctgcattc ctcggtggct atgctgcgcg atttgtacag
     9721 cctcacgaac cagaggaact tatcgatcct ttctcccttc tccgctgtca taggtcactg
     9781 ctcagcagtt ggtaatgcgt tgtcacaggt cactgttttg cagggggtaa tacgctgtaa
     9841 tggttcactg tctggcagcg ggcggggcgc tgtcatcccc gggattttta cccttacggt
     9901 tataggcggt catggtgagc tcgcatccag ccacggcaaa agagagcatc atctccacgg
     9961 cgagcggtat ccttttctcc attatgataa gctcttcagg gctccactcg ccaagcacat
    10021 agtcaacctg cctgcccctg gggaagtcac tcccaatgcc tatcctcagg cgtgcatact
    10081 gatccgtccc aagcacttca ctgatgcttg tcagtccgtt gtgcccgccg gcgcttcccg
    10141 ctgaccttag tctgatgctg ccggtgggta tggccagatc atctgttatg accaggaggt
    10201 tttcaggctc aatcttttcc ttctgcatcc agtaccgcac tgcattaccg ctgcggttca
    10261 tgaaggtgga gggcttgagc aacaccagtt cagcgttttt gtgcctcatc ctggcaacat
    10321 acccgtaacg gctgtcctta aaaacgatac cggatgctgc gactgcagca tccagtacca
    10381 taaatcctac gttatgacgc gtgttcctgt actcgtcgcc aatgttgcca agaccggcga
    10441 taagatgttt cattatgcac ccttaccagg ttgttccttg gccggagccg gagcctcggc
    10501 agccggcgcc tcagctcccg gagcagcagc ttcttcctcc ttgagagaaa ccctggagac
    10561 ttcaaccgct acaatcattg cgcgcttgtt gtcaatgata tccaggttct cataattgag
    10621 atcacctatc ttgaccgact gcccgatacc gaggccggtg atgtcaatgg tcatatgatc
    10681 aggaatatac ttcgtgagcc ccttgacctt cagtgttctg cgcttaacgc gaagcttacc
    10741 cccggccttc acacctgatg actcaccggt gatcttgagc ggaatgtcga ttgtaacagg
    10801 cttgtcttca cttacctgca tgaaatcgat atgctgcaac ctgtcagaaa cagggtggaa
    10861 ctggatttcc ttcatcactg ccttatagct ctttcctgca acttcaaggt tcacaagaaa
    10921 cacatcggga gtatagacca gtccgcggaa agcgttctcg tgtgcataaa aatgaagatt
    10981 ttccttctcc atgtacatca cacatggtac attatcctct gcccgcaggc tgtttgtact
    11041 cttcttgccg aaatgttcac gggggaaggc tttgatctca atagttttca tcagtcttta
    11101 atttgttgtg ctactcaagg ttaatgccat ataacctccc attacacgtt tgacgttttg
    11161 ttggaaaaaa ttggcgtgca aatatagcta aaaagcttag aaaacaaaac ccgaactgat
    11221 agaggtattt gtataaaccg cttttattgc ttcggcgaag atctcgccaa tggaaagcac
    11281 ctttattttc ggcgactggc agatgagggg aatgctgtct gtgacaacga gttcctccag
    11341 cggagacttg tcaatcttct caactgcatt tccggaaagg acggggtggg tggccacggc
    11401 tctcacactg agcgcaccgc ggtccctcat taccgtggcg gcaaaagtaa gcgtcccggc
    11461 cgtgtctacc atatcatcaa tgatcaccac atgcctcccc tcaacttccc cgatgatcga
    11521 aatctcctca accacgttag gcttcttcct cagcttgtag cacagcacca tctcgcactg
    11581 caggtagcgt gcatatgcat tagccctctt tgaaccgccc atgtcagggg cggagacagt
    11641 caggctgggg agattaaggt tcctgatata cggagcgaag agggctgaac cgaagaggtg
    11701 atcgacgggt acgtcgaaga atccctgaat ctggtcagca tgaagatcca tcgtaataat
    11761 cctgtcaaca ccggccttgg tcagcaggtc agcggtgagc ttggctccca gtgacaccct
    11821 cggccggtcc ttgcggtcct gccgtgcata accgtaatac ggtattaccg cattcacctt
    11881 gtaggccgat gccctcttgg cagcgtcaat catcaggagc agctccatca ggttctctgc
    11941 gggaggattg gtagactgga tgatgaacac gtgacaaccc ctgaccgatt cgtcaaaaca
    12001 gggctgaaat tcgccgtcac tgaagctcag caaggagctc ttaccaagct cgatgccgag
    12061 gtggtcggca atcttttctg caattgtcaa tgaagatgtg caggagaata tcttaaaggg
    12121 agcccggccg atcatatcag aaagttggtt ttggtttcac tcaatgatgc cccaaaaata
    12181 atatttatgg gctaatcatt tcatcccggt gaaaaatatc atactgcttt cgatatagct
    12241 gagtacctcc tccctgcccg tacccctgga agcagatgtt acgaaggtag gagggaggct
    12301 ctcccatgat cggagcatga ctctgtcatg gacgtccctg atcttcgctg cttcagcagc
    12361 tgacagcttg tcggtctttg tgaagaccct ggcaaagggt actccgttca gggccagtct
    12421 ctccatgaat gccaggtcag atgccatcgg ctcatgcctt ccatcaatca gcgcgaaaag
    12481 caggatgagg ttctgcctca tagtgacata tgatgtgaag agcctgtccc actgatcacg
    12541 catcttctgt gatacgcgtg catatccata tccgggcagg tcaaccaggt accagctctc
    12601 attgatcacg aaatgattga tggtctgtgt cttgcccgga cttcccgatg tctttgccag
    12661 cccttttttt gagacaagca tgttgatcag ggatgacttg ccaacgttag atctgccaat
    12721 gaatgcatat tccggacggt caggcggcgg aagttgactg atcctggcgc tactggtgac
    12781 aaaggaggct gacctgatga tcattacccg ttcagttttt ccttgaatct ccggttgtca
    12841 ataataaacc tctcatgttt gccttcgcca atcttaccgg tttcgtcatc tgcctcaaca
    12901 tgaaaaagga gcctgtttcc gtttacctcc tgcagggtgg cggtacaggt gatggtcttg
    12961 cccaccgggg tagccttaag gtgcctgatg ttaacttccg tcccgacggt gccctctccc
    13021 tcgtcgaggc aggcttcgac agccttcatc gctgtctgtt ccataaaagc aatcatggcc
    13081 ggtgtggaca gcacatcatg cagtcctgat ccgtaaaccg ctgccgtgtc attcgtgctg
    13141 accgttcttg ttgtggtgag cctgatgcca ggtactagaa tctgtgacat atcatttagt
    13201 ttaagtcttt atatggtaag cgaattgcct gccggtaata gagcctgttg ccaaactgcg
    13261 tcaggtatga tcctataacc gccaccgaat cggcatggtg gcctggtttc ttctgcagat
    13321 cgagataccc cgtggtggcg gtctgcagaa gggggctaag tgagtcgagg tcaaccaggc
    13381 cgttgacgta gtcgcccctg taaaacttca cattctcggc catgtcatcg aactcgatga
    13441 atattttgta gagtcctctc tccgcgtaac ggtcattgac cctgtagcgt attgcgggat
    13501 aaccggtcac cttctctccg ctcatcagga actcttccag cccggggtgg cgtctgccgt
    13561 cagtatagcg gagccgtgcc tttacccccc cgttcttgta gtactgaacc tggtcaccat
    13621 gaattgtatc attgacatag ggcgtgaccc tgaagacctt cccgtcagga tagaaccatt
    13681 tggcttcgcc gtttttgctg tcaccggagt aaggtatctc ctgctccagc tgtcctccct
    13741 tgtaaaaggt acgggtcatc ccttcacgaa taccgttttt atacgtgact tccctgagct
    13801 ttacattatc cctgacgtag ttctttatgc ctgtataacc cgtgtcagcc acctgcacat
    13861 ccttcttcct tccgcttcct tcctggccgg cgcctcctcc gcaggcggag gcaagaatca
    13921 tgataattgc tgctgtaagc agtgtaaact gtttcatttt gatgtggttt tttgtggtac
    13981 gaatacttca agcagagtgg ctgtgcccct ggggattatg gcaacgctgt cagccgaggc
    14041 aggaatgaga atggtctcgc cggcctttat ctcctcactg tggccgttgc actccaccac
    14101 tgccatgctc tgtgtgcaga tgtatatgat gaaggagtca gtcaggctgt aatcctttat
    14161 tatcggcctg tcgaaggtta tcaggctggt atggaaatat tgactttcag ccagcagcac
    14221 tgtccggttg agttcgggga cggttctgat gaggttagtg ctgtcaaagc tgaagtttat
    14281 cgcatcgagg gccagggagg tatgcatctc gcgcggcctg cctgatccgt caacacggtc
    14341 ccagtcgtag atgcgatagg tgacgtcaga tgtctgctgt atctccgtaa gaagtacccc
    14401 gccgccgatt gcgtgcacca gtccggcagg aataaaaaat acatcaccgg ttctgacagg
    14461 agtaacattt atcaggtcaa taagcttctt tgatcccagg tactcaagat attcttccct
    14521 tgtggtcttt ttcctgaagc cggtgtagat tactcccccc ggtttcgtat caattacata
    14581 ccacatctcg gtttttcccc aggcatggtg cctctcagag gcaagcctgt catcaggatg
    14641 gacctgaatt gagagtttgt cggcagcatc aatgaacttc acaagcagcg ggaactccgt
    14701 tccgaatttt gcatagacag cttcacccag cagctcgtcc atgtagatct ccgcaatctc
    14761 ctcgagactg tttcctttca ggaatccgtt ggccaccaac gagctctttc ccggcatgcc
    14821 gcatatttcc cagctctcgc cgatcctggc gcccggcttt gctttcttgc cgaaccgctc
    14881 cctgagggtg tcccctcccc agagcatctc cctgagcatg ggtttgaacc ggatgggata
    14941 aagtgaactc atcagcttca gaaggtatag tcaaccacca gtttggcctt tttgataatg
    15001 gaagctgccg ccacagcctc acggtgaggc tcactcgact ggtaccgcct cagatcatca
    15061 agagtggcaa atgttgcgtc aattacaaaa tccccggcat ggtcaacatc agtgatattt
    15121 acccctgtcc ggtactcaat gacgtacttc agcctgttac ccagcggccc gaagatctcc
    15181 ttcagcttca tcacagactg gttcctctct tcgggagtga ccggcgaggt aagcttaaag
    15241 atcacgatgt gctttaccat acagttgttt ttgttacttt tggtacaaaa ataatcattt
    15301 tatgcttatt gtcgggatcg ccggaggcac aggatcagga aagacaaccg tagtaagaaa
    15361 gctgattgag atgctcccgc cgggggaagt ggtgatattg ccccaggaca gctactatcg
    15421 tgataacagc cacatacctg ttgagcagag gcagaacatc aattttgacc atcctgactc
    15481 ggtggagtgg ccgctgctga tagcccacct tgctgagctg aaagccggca ggccgatcag
    15541 gatgcctgtc tactcatacc ttacctgtgt caggtcagag gagaccgtcc ccatcaggcc
    15601 ggccgaggtg gttgttgtgg aggggatact tgttctcgcc gatccgggcc tgcgccagat
    15661 gcttgacata aaggtattcg tggatgctga tgcagatgac cggctgggca gggtgataca
    15721 gcgtgacata attgaacgcg gccgctcggt gctgatggtc ctcgagcggt ataataaaac
    15781 ggtgaaacca tcacacctac agtttattga gccatcaaag agatatgcag acataataat
    15841 ccccggagga ggcgagaacc ttgtcgggat agaagtgctg aagaccatag ttgagaagca
    15901 cctcaggtaa tgggccccgg gattccggca ggggcggagt tccggtaatg ttttgtcagc
    15961 ttttgcgcag ttcgcgcagg tacatctgaa tggtattttc gagcccgtag tagagggcgt
    16021 cagagatgag cgcgtgccct atggagacct ccataagcca cggaatatga ctgtgcagcc
    16081 atgcaagatt gtgaaggtca aggtcatgac ctgcgtttat gcccagtccg gcatcgcgtg
    16141 ccgcgatggc tgccgtcacg aaaggcgcaa cggctgcttc cctgtcagca tgataccctg
    16201 atgcgtaggg ttcggtatag agctcgatcc ggtcagtacc ggtgcgtgcc gcatattcta
    16261 ttatctccgg atcagtacct gtgaatatgg aagtgcgtat ccccttcccg tgaaaccggg
    16321 caatcacgtc ggtaagaaag gaaaggttct tcctggtatc ccagcctgca ttcgaggtta
    16381 ttgcatcgtg tgcatcaggc accagggtca cctggtcagg caccacgtca agcaccagcc
    16441 tgatgaattg ttcctcagga ttaccctcga tgttgaactc ggtggtgacg atatccctta
    16501 tatcataaac gtcgctgtaa cggatatgcc tctcatcagg cctcgggtga acagtgatcc
    16561 cctcggcacc gaaagcctga cacttcacgg cagccagctt aacatccgga acgttgccgc
    16621 cacgggcatt gcgcagggtg gcaaccttgt tgatatttac actcagtctt gtcatgcctc
    16681 tcttcagctg attccgcggg caaagttata aagatccgca gttttttgta cttttattcg
    16741 ccaaagagcc gctgaatgct tgccaaagac atgatatctg acgtggtccc tgcactgaaa
    16801 acatctgaca cagcgcagac tgcacttaac tggatggaga ttttcagggt gtcacacctc
    16861 cccatcgtga acgagagcga ctacctcggc ctcatctctg acagtgacat ctatgacctc
    16921 aaccagccgg aagagccgat aggaaaccac ccgctctcat tacccagacc ctcggtgaaa
    16981 atacaccagc atatctatga ggtgcttgcc ctcgcagcac ggcacaaact tacagttgtg
    17041 ccggtcattg acgacgacaa tcattaccgc ggcgtcatca cctgtaatga gatcataagc
    17101 ggtatcgctt caatctcagc cattgatcag ccgggcggga tactgatact gaaggtgctt
    17161 cagcgcgact actccatgag ccggatatcg cagatagttg agagcaatga cgcaaggata
    17221 ctcagcatgc acatcacttc tgaccctgac tccaccagcc ttgagataac actgaaggta
    17281 aacaccaccg accttgtttc catcataaaa acatttgaga gatactccta cgaggtcact
    17341 acctgggtga ccgacaatga tgagctggac cgtttctata cagacaggta caacctgctg
    17401 atgaagtacc tgaacatgta acctctgatc acgatggaca aaaccattgc actgtacagc
    17461 aagagcctta ccgaaaaaac cactgcaggc ctgatgaggg tcattgatgc actctatgac
    17521 aggggtgtga ccatatacct gaacatcatg gaccctgaag ggcaccattt caaccatgac
    17581 cacagggtga tactattcag cgagttgtct gatctcccgg catacgtatc tatgatactg
    17641 agcgttggtg gtgacggtac cttccttgag acagccatga gggtacgtac tgccggcatt
    17701 ccggttgctg gcataaacac cgggcgtctt ggcttcctgg caaatattcc cgacaatgaa
    17761 atcggaaaat cagttgatct gatcatatca ggcgattatg aggtgatcag ccgctcgatg
    17821 ctggaaataa cctccccgcc ggaactggca gcggatggta acgccatggc actgaatgag
    17881 ataaccgtac agaaggatga ccagagcatg atcacgatta cggtcgaggt tgacggcacc
    17941 ttcctgaata cttacagggc tgacggattg atcatctcaa cggccacagg atcaacggca
    18001 tacaatctga gtgtgggtgg ccccattctt tatcccacgg atgacagcat aataattgtt
    18061 cccatgtcac cgcataacct caccgtcagg ccaataattg tctccgggag cagtgtcatc
    18121 accctcggca cgggaggccg gagcagtcat tgcctggtta ccattgattc aaaggccaca
    18181 agagtaccgt caggcacagt tcttgaggtg cggcagtccg cttccaggct cagcaccgtg
    18241 accatcgccg gaaatgattt tttcagtacc ctgaggaaca agctcatgtg gggcgccgat
    18301 ccccgtaact gacaataatt aaggaatatt taagttttaa atattgtaca ttccttatat
    18361 ttgtagccgc aattacaatg tgactgatgg ttaagcgtct gatagtgatg tgtatggtgg
    18421 tgtctttcac tgcaccattc gtatcaggtc agctgtggaa gatgaaaaga tatgaagcca
    18481 cggcagctat cggcacatca cagttttacg gagacatagg aggctatacc attggtgaaa
    18541 atgccctggg attcaaagat ataaccttca tccagacgcg ttacagtgtc aatggttcat
    18601 tcaggtactt tatcacggac aatttctctg cgaggctttc attaacgtgg gctgcgcttc
    18661 atgctgatga caggagaggt tcaaataacg atggcaggct ctatgagaca agctcattcc
    18721 tgtatgaacc ggctctgctc ggtgaatact acttcgtcag gaaccgtgaa cgcaacagtt
    18781 tcctcttcca gacctacagt tacaggaggg caaacaggct gcgcgacttt ttccggagcg
    18841 tggatgtcta tgcactgacc ggtatcggcg gagccggtta caacgttttc tacggtaacg
    18901 ataagataca ggaggagaag tgggatctgg atcccaccct cagaaggagc gggtttaccg
    18961 ctgtgattcc gctgggcgtg ggtgccaagc tggccattga tcccaacctc ctggtagggg
    19021 tggaactggc aggcaggtat gcgctctctg attatcttga cgggtatacc tccgtgtact
    19081 cgaagagaaa tgacgcctac cataccttca gcgtaacagt gagctacagg attaaaaccg
    19141 cacgcaacgg ccttcccgat ttcagaacgt tgcgccaatg accacggtaa tgaaaagatc
    19201 aatcataata accatactga taattctggc tcttccggct ttgccccagc gcaccgctga
    19261 ttacggcgtc tcagccgggg tggtgaacta cctcggagag cagaaccctc tcagcgcttt
    19321 tttcaagccc ggtcctgcag tgcagctgtt ttaccgttac aacttaaacc ccaggcaggc
    19381 cgtaagggca aacctccttg gcggttacat gaccccgctg ggttcattcc tgggagagct
    19441 gggcgcaact tatgaattca acttcttccc atactccaca ttcaggagca ggcatattga
    19501 ctattcacca tatatcgccg gaggcgcggc cctctcaatg atgagccagg ccggcccatt
    19561 cctctctgtt ccattctctg ccggttttaa ggtcaacgtg gctaaccagg ttggcgttga
    19621 aatcgaatat ggattccgca agacttttac cgataaactg gatggccaat tcgagataac
    19681 agattccggt ggcgaaccgg ttgacagaac atggacccac aataatgact ggtacagttt
    19741 cctgggggtc tcagtcacat ggaagatcta caacaagctt gcaggctgtc cagcctttgc
    19801 agagatggga aaagataaaa acagaaagag gagatagcgt tgacttataa ggatcagata
    19861 gacatgagca ggttgccggg gcatgtggct gtcattatgg acggc
//
LOCUS       DAOPXU010000046        19417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_2940_length_19417_cov_15.314275, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000046 DAOPXU010000000
VERSION     DAOPXU010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19417
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2940_length_19417_cov_15.314275"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PKK03_06340"
     CDS             complement(<1..229)
                     /locus_tag="PKK03_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84055.1"
                     /translation="MSEPILKALIQLFALIGDVHDDPEISGRGRDIVRLFLAKHLSNE
                     QVLRYMAMFDEYVRLYHPDVLSKDSIRDRKRT"
     gene            432..833
                     /locus_tag="PKK03_06345"
     CDS             432..833
                     /locus_tag="PKK03_06345"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HNX84056.1"
                     /translation="MDKLILSPTVFTPRVILDHEEHYLEISGESRPNDVREFFGPIIQ
                     WMNDYSESLQRSGNLGKPVVSHFNFNYFNSASAKCILDICKILARIKSGGTDISVKWY
                     YVMGDDDMKETGREMEMIVNLPFELIEIQSQ"
     gene            974..2656
                     /locus_tag="PKK03_06350"
     CDS             974..2656
                     /locus_tag="PKK03_06350"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNX84057.1"
                     /translation="MPEKGFWQVYAEGIPSKYNSLGLIIKPFGEFCRTCIITEEEINA
                     MVSTDIERFAGQSGIAERRGFFLTLNYLIPACLKEAGCEIVGQEEAEGVTVSLARKIA
                     RAIHAGYLREMNRQSSSIPGANAVRVAEFDDLPGEIRESNISNALHIPSKLLAIGYRI
                     RPVRKGYKAKALHLDDEEIETMARVEHLRWSWEKRLCGWTYGKTRDERHKRHPNLVPY
                     GDLTDDEKQKDRELVRMVPGILRDIRFEAYPVSSGHTARLAYALKPRSSIQKLLLGTN
                     DLSAEISLLTGNSPSIMEKLRSIDDRIRLTINEVEGSYNYARYIQDAFLPGDLDIREC
                     FTDSFVLFEPRDIVSGDFYLFSRQEGMVTFGLADCTGHGIPAALISTIGYGILDQSVN
                     ILKIDDPSEALSNLYSGIHRFLRKDDRESGLPDDMTIVLCRFDPVSMRLYHAGAGNAI
                     FHVTDGKISGNLSGMLSGGQLSNGSYGFKTRTLQLDTHDVLYLCSDGFADQFGGGNHR
                     RYGWNRLRDFLLEISGHPMAEQGDMLYEELEQWRGETDEDQTDDITVIGIRV"
     gene            2767..5154
                     /locus_tag="PKK03_06355"
     CDS             2767..5154
                     /locus_tag="PKK03_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX84058.1"
                     /translation="MASLVPGYEYDIFISYRQKDNRYDGWVTEFVDHLKSELEATFKE
                     EISVWFDLNPHDGLLDAHDVDESLREKLKCLVFIPVISRTYCDPRSFAWEHEFMAFMG
                     MAAGDRFGLKVKLPAGNVASRVLPVQIHELKPDDRSLVEEALGGFLRPIEFVYAEPGV
                     NRPLTPGDSAEKNLHKISYRNQVNKVANTIDEVISGIVRAAAAQSADQLAGTTPPSEI
                     RAEKHVGGRLKANREKWVSIGSGSLIIVLALISLYLFSGGTRLPFSERDWILISDFEN
                     NTGNPVFDKSLYTAFSISAGQSRYANVFTRSRMLEALSRMKMAGLKFIDEPTGREIAL
                     REGISLMIVPGISEAGNQYAITAKIIETKTGNSLRSEILYAANEAEILPVIDKLSRKI
                     RKDLGESRYDISLQDKPLTRVTTSSLPALQQYSLGHESHLFSDFRRAREFYENALRAD
                     TGFVAAKASLGNILIQKFNDSSGKALVRQAVRDAGNLTDRERYSILAFYAENIEHDFD
                     KAIANMEILKRLYPDDPVIHNNLGWYHQQNGQYELALPEYKQAVRVNPHMILTYSGIL
                     WIYGEYIGNADSVDVWAKKMVADNPDSPWAYYYMASAWLFRDSIEKSIECHQKARELS
                     PGLMINNYRLGHTYMESGRYDEAIEVFEEILAIDPEEYSAYYNLGCCHEALGNQPDAR
                     ACFLKFRKNAEEKWLKHYPDYYATYTTLASVAARLDEMELSRQLLNKAVELDSAQYFS
                     FAEVLCVQGEIPEAIDNFELALEHGYRSLYGLKGNPELAALRFDIRLKNLLDKYF"
     gene            5309..7777
                     /locus_tag="PKK03_06360"
     CDS             5309..7777
                     /locus_tag="PKK03_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84059.1"
                     /translation="MASLIAGYGYDIFISYRQNDNRHDGWVTRFVDHLKGELEATFKE
                     EVSVYFDLNPHNGLLDTHDVDESLKEKLKCLIFIPVISRTYCDPKSFAWEHEFRAFAG
                     MASNDSLGLKVTLHSGNVASRILPVRIHELDDEDNRLIEKETGGVLRAIDFVYREPGV
                     NRPLTPDDDEHRNLSGTRYRNQINKTANAVREIILALQSEHHPAHREKLSGKEPWEEP
                     VKAHAGNGKRKISELLTPKRLLTLLVPVLAIGLSLLLLSRFAGRPGRPAGINEKSIAV
                     LPFEIIGSGNGNIWLGDALTDEIISQLCKINDLEVRPRTSVMRYRATTISIPAIGREL
                     GTNYIIEGNCQIFNEVVRITVKLINARKDQQLWSDVFEGTWDSLHGMQTDIAIKTAAT
                     LQAVLTPEEKARIEKNPTGSAGAYRNFLKANVLSDNAQYFLLAGNRFVDSISFTAAIS
                     MYDKAIAEDPGFALAYARRAIARSWGYYSGQLDDSHLLKCKADADRAFALDNNLPDAR
                     IALGFYNYYCMRDYREALSQFSRAAETDPADYQPPFYMAMVYRRMGEWQKSQDLLRKV
                     MMEDPRDAIVLINIGTSFVFMHSFDTALVQFSKAINVMPEWPGPYINAIDACLLKDGN
                     TTMAREMLDSLTARTGRTDNYFRIFLNICDGRYRDALNDLQSSSNDDFESPGLRYLLS
                     GLSYGLINDEAMTTAYYDSALVTYKKLIMENPDDYYSHSCIGLAYAGLGNATDAVIAG
                     KTAVQLAGDNYLVKHDMIVNLAKIYAMTGNYPEAIRQVEYLLTNPSWFSLNIIRIDPS
                     WGKLSETPEFRTMARGTGNYKKPL"
     gene            7800..8015
                     /locus_tag="PKK03_06365"
     CDS             7800..8015
                     /locus_tag="PKK03_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84060.1"
                     /translation="MTSFDDGEMFRPFPRPFPMHAVQYFSPQDLNPAQRKALATMLLE
                     FYQKCAMEEQKLITGMISVINNLESKG"
     gene            8036..9586
                     /locus_tag="PKK03_06370"
     CDS             8036..9586
                     /locus_tag="PKK03_06370"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX84061.1"
                     /translation="MLVAFGEEENLGVGYLMSVLQGAGITSRMIDIRYDSEEILAAIK
                     RESPVVVGFSVIYETCLDGFTELVSYLRKGGVDCHFTAGGHYASLYPEELFGFIPGLD
                     SVVRFEGEYTFPELVNCLVTASDWKGLRNLAYRENGRVIRTPLREPEKNLDNFPYPYR
                     RPLREYALGKRYSTIISGRGCTYSCSFCNTGEFYRQAGGPLKRIRRPEAVVDEMLRLY
                     SESGCRIFLFQDDDNPVKTAGGNLWIKSFCSEIERTGLLGRVIWKMNCRPDEIEPEIF
                     SIMKRHGLFLVFIGLEDGTDEGLKRFNKKMTVESARYGVEVLRQLGIGFDYGYILFQP
                     ESTFSSLRESLRFLEEICRDNLVPITFLKLIPYFGTRVERELREAGRLTGRPGNFDYS
                     YLTPALDACWGAVLNCFDEWLWGRRGAVNLAKWVRNCLAVRDHFGKPDEIVERQRSSY
                     CETVAQGNQFLAETLYGLFDCFESGRHLSEGQERIERIALESARRHDIICGTFRENLR
                     ALQEESWR"
     gene            complement(9667..11910)
                     /locus_tag="PKK03_06375"
     CDS             complement(9667..11910)
                     /locus_tag="PKK03_06375"
                     /inference="COORDINATES: protein motif:HMM:NF027726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusF/SusE family outer membrane protein"
                     /protein_id="HNX84062.1"
                     /translation="MKAKIFTQVAAVMLFMALLPGNAQELLTDGGFDTTAEIPLVTGD
                     PSPDNAWYLYQSPYVTASAYIESGICVYNIPADGYPGDYAWEIQLIQSGFVLQQTHMY
                     RLSYDVMADAERDYGLFLGEAGGNWNSLIGYDKYTQYATTEWKTVVIDFKTTLIFPYH
                     KLSFEVGAIPVSMYFDNVSLTDMGPYTPSVGILGSSLSGWDNDVDMQTADGINYAVQL
                     YLATGRVKFRQDDLWWINWGGTEFPAGTGSLYGSDIMVTNPGTYDITFNRETLEYSFT
                     CVDNCSAFLGIAGTAVPPWQDWNTDVNMITYDGITYMMTAYQFTDGEAKFRKNDSWTE
                     NWSNNTFPSGTAIPDGPPIPVTAGNYKVTFNILTGEYNFAFPSIGILGDALPGWWYDD
                     IDLTTTDGIIYTLTDYPFNQGEVKFRMENEWDVNWGDYGFPTGYGYQDGPNIQVPAGT
                     YTVTFNRLTGEYAFMATTCQVPGIICPSFIYAPSDPGICGAWIYYPDVMPAPNCGGEG
                     VTMTQTTGLPSGSFFPAGMTTNTFVLTNAEGATATCSFDVYVWDNEAPVVSGLKENYD
                     PLWPPDHNMVQVPLDYQVYDNCGSTECQLYVWSSEPDNGTGDGDTAPDWEIIDGQTVM
                     LRAERSGTGTGREYHIIIECRDMSWNFSYNEIVVKVPHDINGKAPFITFIWPNPTDYN
                     FNLQVESDSDNPIQVSVSDATGRVLSKYQVRNNETISFGSDLMPGSYFVIVKQGSNSE
                     TILVLRQ"
     gene            complement(11944..13149)
                     /locus_tag="PKK03_06380"
     CDS             complement(11944..13149)
                     /locus_tag="PKK03_06380"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNX84063.1"
                     /translation="MKNPGILIATLLVMLPGFVAKAQVTTDKTKSAETESLAQKIVSQ
                     CANIKEGEFVLIQGGVREMELLEDIAINVAKKGGYPLLTVGSDRLTRMFYTEVPVKYD
                     LRAPDLDYKILCFIDAAIGVNYNEDPLLLADIPAERLAAISQANAPVTDLAFSRKIRQ
                     VSIGNGLYPTEARAKQFGITFKELSDIFWKGLNVDYQALETTGNSFKKVLEAGNEVHI
                     TNPNGTDLKVKITGRKTVVLDGIVSDEDIKEGVLATQVYLPAGEVALAPVPGTAEGKI
                     VIASDFGSGVEVKNITLIFQKGKLVSMTAESGIERLRELYDASAERKDEFSSIDIGIN
                     PNVSIIPGSKLACWMPAGMITIGIGNNLFAGGDNKSTFGYNFHIPGSTLTVDGNVLVD
                     GGALVPVKK"
     gene            complement(13297..13485)
                     /locus_tag="PKK03_06385"
     CDS             complement(13297..13485)
                     /locus_tag="PKK03_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84064.1"
                     /translation="MKTKTAKRKLSRIYIALIFLLIPGFNSCEIFYCEKCTMGDETFW
                     ECSQDAIEMWESSGYSCE"
     gene            complement(13550..14257)
                     /locus_tag="PKK03_06390"
     CDS             complement(13550..14257)
                     /locus_tag="PKK03_06390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HNX84065.1"
                     /translation="MKTKSKIRMLPLILTGISLLFINGCQKEEESSEIKDKDGNIYTS
                     LTIGSQVWMAENLKTTKYNDGTAIPLMTYSYIKNPGYTWCEEDAALYKESYGALYNWY
                     AVNTKKLCPSGWHVPTDAEWHTLILNLDGNAQNVTGIESETAGGKLKETGTAHWKTTS
                     TSVTNESGFNGLPGGHCHIGDPHAFYYMGYRGLWWTATEFNDGSAWLRDLRDNDIKVY
                     RGYLDKGYGFSVRCIKN"
     gene            14476..14793
                     /locus_tag="PKK03_06395"
     CDS             14476..14793
                     /locus_tag="PKK03_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84066.1"
                     /translation="MVAAKKSGRFPWGWIVLILVVVLLYSTNPSEPQFTSYLKDRIRE
                     QVKGDETMSGDLARLLSGPAASLAGLGTVRKDYFLCSTYELSLPGEEHLFLGIMDHFV
                     KLK"
     gene            complement(14811..16148)
                     /locus_tag="PKK03_06400"
     CDS             complement(14811..16148)
                     /locus_tag="PKK03_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNX84067.1"
                     /translation="MDKRTVNRRIFLSKSTACMAGVGIAGSGAMFGREISPREQLQPE
                     IREYRTLGRTGFKVSDIGCGFPGTASESVLKAAVAGGINFIDSIEGHGGGNSEVTIGR
                     AIKTCPRESLFINSKIAVTEKDTRETLVSRVKKTLERLDTPYIDGLMLWSANTVIATE
                     NKVFHSAVRQMKKEGIVRFSGVSCHGSSFTGEPPETMDKVICAAVDSGRFDLVMFVYN
                     YVQQEMGDKILEQCQKKNVGTTLMKTDPFGGAYNIVTNMVTNYQKENKEIPPNIQNVY
                     SKIMKSQEQAMPFLIQHGLSDINARKEAAISFVLNHPAVSTVLISFSNFTEVADYINL
                     SGSRLTEKSQSMVDSMRDIYGPFYCRHACGICESSCPAGVPVNTIMRFNHYFMAQSRE
                     KFAISQYLGLGRPDAGNCRNCKGFCEAACPYGVPIHSLLEDAHKNLSIAGEYI"
     gene            complement(16382..16591)
                     /locus_tag="PKK03_06405"
     CDS             complement(16382..16591)
                     /locus_tag="PKK03_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84068.1"
                     /translation="MGIEELDHINDKAHYLFEPAVTFRVGWENIKLQAQLSRSLYLGD
                     HSYFSFEEIHLSGGVVFNIPAEKRD"
     gene            complement(16593..16979)
                     /locus_tag="PKK03_06410"
     CDS             complement(16593..16979)
                     /locus_tag="PKK03_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84069.1"
                     /translation="MNKILLGAILTAIVHCGCSHYYYVANVQNVPLFQEKEDLHISAF
                     LGAGDESFSIEAQTAYPAGKKIGLMADFMTAWGGKASDNDFGRGIYLDGAIGYFTPLG
                     QAFVFEVYGGAGISSHLPPFLLSRNQ"
     gene            17247..17837
                     /locus_tag="PKK03_06415"
     CDS             17247..17837
                     /locus_tag="PKK03_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84070.1"
                     /translation="MNLNVSVGKISDRMPEDFINYLSSVGLVIESGLLVLPHKNHYFY
                     DFEELKRVKTIVNLMKLNQVRELRGFVRKLSELLHYQANFVGCFIDNKIYNPFSFKYR
                     RLTEDPSELPEFYETGIESRIPFVNRIYNFIDAKTDRSLSRKTVTNLLNEFGFEVVIM
                     TEQNGVTYFHSRKVGHEPLQLSPEKLHSLKRKEIPS"
     gene            complement(18210..18473)
                     /locus_tag="PKK03_06420"
     CDS             complement(18210..18473)
                     /locus_tag="PKK03_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018712249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HNX84071.1"
                     /translation="MKRITFSKNAWEDYLSWQAEDRKILKKINTLIKDIQSTPHSGIG
                     KPEPLKYDLAGLWSRRIDHEHRLVYQVNEDELLIYSCRYHYDK"
     gene            complement(18470..18724)
                     /locus_tag="PKK03_06425"
     CDS             complement(18470..18724)
                     /locus_tag="PKK03_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNX84072.1"
                     /translation="MIAANYSEFRKELKDYLDSVELNNETLIIKRGSGKGAVLISLEE
                     YNSIMETLHLLSSKKNAQRLYESIEQMQSGKTFQGNLIEE"
BASE COUNT         5074 a         4511 c         4674 g         5158 t
ORIGIN      
        1 aagtccgttt cctgtcacgg atgctgtctt tcgataatac atcagggtgg taaagcctga
       61 catattcgtc gaacatggcc atgtacctca atacctgctc attactcagg tgttttgcca
      121 ggaaaagcct gacaatgtcc cttcccctgc ctgatatctc aggatcatcg tgaacatcac
      181 ctatcagtgc gaaaagctga atcagggctt tcagaatggg ttcactcatc ccggttgatt
      241 tattttataa accgccgctt cttttagtgc tgctcccccc ctcaaatccg tccggaatct
      301 tatctttgtt caagatattc aagttactaa taaaaaaata catgaaggga gaatttcaga
      361 ttaatcctgt tgcgatgccc ggaaaggctg cggaccgtgt attttcccgg gcgacttaat
      421 gaggaactcc tatggacaag ctgattctca gcccgactgt tttcacgccc agggtcattc
      481 ttgatcatga agagcattat cttgaaatct caggagagtc aaggccaaac gacgtacgcg
      541 agttcttcgg acctatcatt cagtggatga atgattacag cgagtcgctt cagagatcag
      601 gcaacctcgg caaacctgtt gtctcgcatt ttaacttcaa ttacttcaat tctgcatctg
      661 cgaaatgcat tctcgatatt tgcaagatac ttgccaggat aaagtcagga ggaacggaca
      721 tctcagttaa atggtattat gtcatgggtg atgatgacat gaaggagaca ggcagggaga
      781 tggaaatgat tgtaaaccta cccttcgaac tgatagagat tcagagtcaa tgacagcaga
      841 cggaggacag gctgacccgg tctggttcag tgagcaactt gccccattgg tcgcggccag
      901 cctttgcaga catttcaaaa gagtttccgc gggcggcagg ggtcacccag taaggacccg
      961 ccatgatcac gcaatgccag aaaagggatt ctggcaggta tatgcagaag ggattccctc
     1021 aaagtataat tctctggggc ttatcataaa acctttcgga gaattctgca ggacctgcat
     1081 cattaccgaa gaggagataa atgccatggt atcgacggat attgaaaggt tcgccggtca
     1141 gtctggcatc gcggagcgaa gaggtttctt tctcaccctg aattatctca tcccggcctg
     1201 cctgaaggag gccggctgtg agatcgtcgg acaggaagag gcagagggtg tgaccgtctc
     1261 actggcgcgg aagatagccc gggcgattca tgcaggatac ctgcgcgaga tgaacaggca
     1321 gtcatcctca atacccgggg caaatgccgt cagagttgca gaatttgatg atctgccggg
     1381 tgagatccgt gagtcaaaca taagcaatgc gcttcatata ccttccaaac tgctcgccat
     1441 aggttacagg ataaggcctg tcaggaaggg ctataaagca aaggctttgc atcttgatga
     1501 cgaagaaatt gaaactatgg ccagggtgga gcatctcagg tggagctggg agaaaaggct
     1561 ctgcgggtgg acatacggaa agacaaggga tgagcgacat aaaaggcatc ctaacctggt
     1621 gccttacgga gacctgactg atgatgagaa gcaaaaggat cgtgagctgg tacggatggt
     1681 gccgggcata ctccgggaca taagatttga agcatacccg gtatcatccg gacatacagc
     1741 caggctggcg tatgccctta aaccacggag cagcattcag aagcttctgc tgggtacaaa
     1801 tgacctcagc gcagagataa gcctccttac cgggaattcg ccctcaatca tggaaaagct
     1861 gagatcaatc gatgacagga tcaggcttac gataaatgag gtggagggca gctacaacta
     1921 tgccaggtac atacaggatg cttttctgcc gggtgacctt gatatcaggg aatgcttcac
     1981 cgacagtttt gtcctgttcg agcccagaga tatcgtaagc ggtgatttct acctcttcag
     2041 caggcaggag ggcatggtta cctttggtct ggctgattgt acagggcatg gcataccggc
     2101 ggcacttata agcacaatcg gatacggaat ccttgaccag tcagtgaata tcctgaagat
     2161 tgacgatccc tcagaggcac tttcaaatct ctactccggc attcacaggt tcctgcggaa
     2221 agatgaccgg gaatcagggc tgccggatga tatgacaatc gttctgtgca ggtttgaccc
     2281 ggtttcgatg aggctgtacc atgccggtgc cggaaatgca atttttcatg tcactgacgg
     2341 gaaaatctcg ggaaatctct cagggatgct ctccgggggc cagctcagca acggaagcta
     2401 tggttttaag accaggacac ttcagcttga cacgcatgac gtgctctatc tctgctctga
     2461 cggatttgca gatcagtttg gaggcggcaa tcaccggcgg tacggctgga accggcttcg
     2521 tgacttcctg cttgaaataa gcgggcatcc catggctgag cagggtgaca tgctgtatga
     2581 ggaacttgag caatggcggg gcgagacgga tgaagaccag acggacgata ttacagtcat
     2641 cggcatcagg gtgtgacggt ttttgctgcg ggggctcctg aaacctgacc ggaccccgga
     2701 cggtgccatt actttatttg acatggccct tttagtttcc taactttgat aaaatgaatt
     2761 tattagatgg ccagccttgt cccgggatat gagtatgaca tcttcatcag ctaccggcag
     2821 aaagataaca ggtatgacgg ctgggtcact gaatttgttg atcatcttaa gagcgaactg
     2881 gaagccacct tcaaggaaga gataagtgtc tggttcgatc tgaatccgca tgacggtctg
     2941 cttgatgcac atgatgtgga tgaatcactc agggagaaac tgaaatgcct cgtctttatt
     3001 ccggtaatat cccgaaccta ctgtgacccc agatccttcg catgggagca tgagttcatg
     3061 gcctttatgg ggatggctgc cggcgacagg ttcggactga aggtgaaact ccctgccggg
     3121 aatgtggcat ccagggtctt gccggttcag atccatgagc ttaaacctga tgacaggtct
     3181 cttgttgagg aggccctggg cggatttctc aggccgatag aatttgttta cgccgaaccg
     3241 ggcgtaaacc gccctcttac gccgggagat tctgctgaaa agaaccttca caaaatcagt
     3301 taccgcaacc aggtcaacaa ggttgccaat acaatagatg aagttatctc aggcattgtc
     3361 agggcagcag cagcccaatc agcggatcag ttggccggaa ccacaccgcc atctgagatc
     3421 cgggcagaaa aacatgtcgg cggcagactc aaggcaaacc gggaaaaatg ggtgtcaata
     3481 ggttcaggtt ccctgattat cgtcctggcg ctcatttccc tgtatctgtt ctcgggtgga
     3541 accagactgc cgttctccga gcgcgactgg atactgattt cagatttcga gaacaacacc
     3601 ggcaatcctg tttttgacaa gtcgctttac accgcctttt ccatctcagc cggtcagtcg
     3661 aggtatgcca atgtttttac caggtcaagg atgctcgaag cattgtcaag gatgaaaatg
     3721 gcaggtctga aattcatcga cgagcctacc ggcagggaga tcgccctgcg tgaagggatc
     3781 agcctcatga tagtgcccgg cataagtgaa gccgggaatc agtatgccat cacggcaaaa
     3841 atcattgaaa cgaaaacggg caattccctg agatcagaaa tcctgtatgc tgcgaacgag
     3901 gctgagatac tgcccgtgat cgacaaatta agcaggaaga tccgcaaaga ccttggtgag
     3961 tcgagatacg atatttctct tcaggacaag cccctgacaa gagtaactac ctcatctctt
     4021 ccagccctgc aacaatattc gctggggcat gagagccatc tcttttctga tttcaggagg
     4081 gcaagggaat tctatgagaa tgcactcagg gcagacacgg gatttgtggc agcaaaagca
     4141 tcactgggca atattttaat tcagaaattc aatgattctt ccgggaaagc gctggtgagg
     4201 caggcggtaa gggatgccgg aaacctgact gaccgggaac ggtacagcat acttgccttt
     4261 tatgctgaaa atattgaaca tgacttcgat aaggcaattg ccaatatgga aatattaaag
     4321 aggctctatc ctgatgaccc tgtaattcat aataatctcg gctggtacca tcagcagaac
     4381 gggcagtacg aactggcact gccggaatac aaacaggcag tcagggtaaa tcctcacatg
     4441 atcctgacct attccggcat tctctggata tacggagagt acatcggcaa tgctgattcg
     4501 gtggatgtct gggcaaaaaa aatggttgca gacaatcccg acagtccatg ggcatattac
     4561 tatatggcat cggcatggtt gttccgtgac agtatagaga aaagcataga atgccatcaa
     4621 aaggccagag agttatcgcc gggtttaatg ataaataatt accggctggg ccacacttac
     4681 atggaatcgg ggaggtatga cgaagcaata gaggtctttg aagaaatcct ggccattgat
     4741 ccggaagagt actctgcata ttataatctt ggttgttgtc atgaagccct gggaaatcag
     4801 ccggacgcaa gagcctgctt cctgaagttc aggaaaaatg ctgaggaaaa atggttgaaa
     4861 cactaccctg actattacgc aacctataca acactcgcct ctgtggctgc caggcttgac
     4921 gagatggaac tgtcgcggca gctgctgaat aaagcagttg aacttgattc tgcacagtat
     4981 ttttccttcg cagaagtgct gtgtgtccag ggcgaaatcc cggaagccat tgataatttt
     5041 gaacttgccc ttgagcatgg ctacaggagt ctatatgggc ttaaaggcaa tcccgaactg
     5101 gcagcgctcc ggtttgacat ccgccttaaa aacctgctgg ataaatattt ctgacctgac
     5161 tcaatggttc acaccggggc gcagcaggtt aagtttatac agcattgaca atgaactgat
     5221 ttaatatatt ttgagaagga gggtaacggc tgaaaatatt tggtttgcct tgtgcgcttt
     5281 cgtaacttta taaaaatgga cggataacat ggcctcactt atagcgggat acgggtatga
     5341 tatctttatc agctaccgcc agaatgataa caggcacgac ggatgggtca cccggttcgt
     5401 ggaccatctg aagggagagc ttgaagccac attcaaggag gaggtctccg tttactttga
     5461 cctgaatcct cataacggcc ttcttgatac tcatgacgtt gacgaatcgc tgaaggaaaa
     5521 gctgaaatgc ctgatcttta ttccggttat atcccggacc tactgtgacc ccaaatcgtt
     5581 tgcatgggaa catgagttca gggcgtttgc agggatggct tccaatgaca gtctcgggct
     5641 gaaggttact ctgcacagcg gcaacgtggc aagccggata ctgcccgtac ggatacatga
     5701 gctcgatgat gaggacaaca ggctgattga gaaggaaacc ggaggcgtac tcagggctat
     5761 tgattttgtc taccgggaac ccggagtcaa ccgacctctc acacctgatg atgacgagca
     5821 caggaacctg tcaggaacca ggtaccgcaa tcagatcaat aagacagcca atgctgtcag
     5881 ggagataatc ctggccttgc agtcagaaca tcacccggca caccgggaaa aactatccgg
     5941 gaaagaaccg tgggaagagc ctgtgaaggc gcatgccggt aacgggaagc ggaaaatcag
     6001 cgaattgttg acgccgaaac gtctcctcac tcttctggtg ccggttcttg caataggatt
     6061 gtctctgctt ttgctgtcga ggtttgccgg ccgccccgga agaccggcag gaatcaatga
     6121 aaaatcaata gcagtgcttc cgttcgagat tatcgggtcc ggaaacggga atatatggct
     6181 gggcgatgca cttacagatg agatcatttc gcagctgtgc aagatcaatg acctggaggt
     6241 acggcccagg acttccgtca tgaggtacag ggcaacaacc atatcaatac ctgctatcgg
     6301 gcgcgagctg ggcacaaatt atatcattga aggtaactgt cagatattca atgaagttgt
     6361 taggataacg gtaaaactca taaatgcccg gaaagatcag cagctctgga gtgacgtctt
     6421 tgagggtaca tgggactcac tgcacgggat gcagacagac atagccatta agactgcagc
     6481 caccctacag gcggtgctca cccctgaaga aaaagcacgt attgagaaaa atcctaccgg
     6541 aagtgcaggg gcctaccgta atttcctgaa agccaatgtt ctttctgaca atgcccagta
     6601 ttttctgctc gcgggtaacc ggtttgttga ctccataagc tttactgctg ccatctcgat
     6661 gtacgataag gcaattgcag aggaccctgg ctttgccctt gcctatgcca ggagggccat
     6721 agcacgttca tggggatact attcaggtca gcttgatgat tcgcacctgc taaaatgcaa
     6781 ggctgacgct gacagggcgt tcgcacttga caataacctg cctgatgccc gcattgcgct
     6841 gggattttac aactactact gcatgaggga ttaccgggaa gcgctctccc agttcagccg
     6901 ggcagctgag acagaccccg cagattatca gcctccgttc tatatggcca tggtctaccg
     6961 caggatgggc gaatggcaga aatcacagga tctcctgagg aaggtaatga tggaagatcc
     7021 acgggatgcg attgtgctta tcaatattgg aacatcattt gtctttatgc acagtttcga
     7081 tactgccctt gttcagttca gcaaagcaat aaatgtcatg ccggaatggc ccggacccta
     7141 catcaatgcg atagatgcct gcctgctgaa agacgggaat accaccatgg cgcgggagat
     7201 gcttgactcc ctcacggcca ggaccggccg gacagataat tatttcagga tcttcctgaa
     7261 tatctgcgat ggcaggtacc gcgatgccct gaatgattta cagtcttcat ccaatgatga
     7321 cttcgagtct ccgggactcc ggtacctgtt gagcgggctg tcatacggcc ttatcaatga
     7381 tgaggcaatg actacggcgt actatgattc tgcactggtg acatacaaga agctaataat
     7441 ggagaacccg gacgactatt attctcacag ttgcatcgga ctggcatacg ccggcctggg
     7501 aaatgcaact gacgcagtca ttgccggaaa aaccgcagtg cagctcgccg gtgacaacta
     7561 cctggtaaaa catgacatga ttgtcaacct ggccaaaata tatgccatga ccggaaacta
     7621 tcccgaggca atacgtcagg tggaatatct tctgaccaat ccctcatggt tctcactgaa
     7681 catcatccgg attgatccgt catggggaaa gctgtcagaa acccctgagt tcaggacaat
     7741 ggcaaggggg acaggcaatt ataaaaaacc tctttagtat aaacttaaaa aagcaagtca
     7801 tgacaagttt cgatgacggg gaaatgttca ggccttttcc aagacctttc ccgatgcatg
     7861 ctgtgcagta tttcagtccg caggatctta atccggcgca gcggaaagct ctggcgacca
     7921 tgctgcttga gttttatcag aaatgtgcaa tggaggaaca gaaactgatc acaggtatga
     7981 tcagtgtaat aaacaacctc gagagcaagg gttgaatcct tccttgaaac ctgttttgtt
     8041 agttgccttc ggcgaagaag aaaacctggg ggtggggtat cttatgtccg tgctgcaggg
     8101 agcaggaatt accagccgta tgatcgatat acggtatgac agtgaagaaa tacttgcagc
     8161 cataaaaaga gagagtcctg ttgtggtggg attctcggtg atctatgaaa cgtgcctcga
     8221 cggtttcacg gagcttgtca gctatctcag aaagggtggt gttgactgtc acttcactgc
     8281 cggaggccat tacgccagcc tctatcccga agaactcttc ggatttattc caggccttga
     8341 ttctgttgtc aggtttgagg gcgagtacac attccctgag cttgtaaatt gcctcgtgac
     8401 agcctctgac tggaaaggac tcagaaacct ggcgtaccgc gaaaatggga gggtaatcag
     8461 aacacctctg cgggaaccgg agaagaacct tgacaatttc ccgtatcctt accggaggcc
     8521 cctccgggag tatgcgctgg gtaaacgata ctcaacaata atttccggaa gaggatgtac
     8581 atatagctgt tccttctgca ataccgggga gttctacagg caggccggcg gaccacttaa
     8641 gcgaataagg aggccggagg ctgtcgtgga tgagatgttg cgcctctact cagaaagcgg
     8701 atgcaggatt tttctttttc aggatgatga caacccggtg aaaactgccg gcggcaatct
     8761 atggataaag tcgttctgca gtgaaataga gcggacaggc cttctgggcc gggtaatctg
     8821 gaagatgaac tgcaggcctg atgaaattga gccggagatt ttttcaatca tgaaaaggca
     8881 tggactcttt ctcgtattca tcggattgga agacgggacc gacgaggggc tgaaacggtt
     8941 taacaagaag atgactgtgg aatctgcaag gtatggcgtt gaggttctcc ggcagctcgg
     9001 aatcggattt gattacggtt atattctttt ccagcctgaa tcgactttct catcactcag
     9061 ggagagcctg cgtttccttg aggagatctg cagggataac cttgttccca ttacctttct
     9121 caagctcata ccttacttcg gtacccgggt ggaaagagaa ttaagggaag ccggccggct
     9181 gaccggcagg ccggggaatt tcgattatag ttacctgact ccggcccttg acgcctgctg
     9241 gggtgcagtt ttaaattgct ttgatgagtg gctgtggggc cgcaggggtg cagtgaatct
     9301 tgcaaaatgg gtcaggaatt gccttgcagt ccgggatcat ttcggaaaac cagatgaaat
     9361 tgttgagaga cagaggtcat catactgcga gaccgtcgct cagggcaatc aatttctggc
     9421 tgagaccctg tacggattgt tcgactgttt tgagtcaggc aggcaccttt cagaagggca
     9481 ggaaagaatc gaaagaatag cgctggaatc tgccagaaga cacgatatta tctgcggaac
     9541 cttcagggaa aacctccggg cactccagga ggaatcatgg cgctgaatga aaagatcctg
     9601 caaacatacg gttcgcagga tcttaacctc ataaaatact caacaagaga attatttcag
     9661 cagcaatcac tgcctgagaa ccaatattgt ttctgaattg cttccctgtt tgactattac
     9721 aaaatagcta cccggcatga ggtcactgcc aaaactgatc gtctcgttat tcctgacctg
     9781 gtattttgag agaactctcc ctgtagcatc gctaaccgag acctgaatgg gattatcaga
     9841 atctgactcc acctgcaggt taaagttgta atcagtcggg tttggccaga tgaaggttat
     9901 gaaaggcgct ttcccgttaa tgtcatgagg taccttcacc actatctcat tatatgagaa
     9961 attccatgac atgtcacggc attcgatgat aatatggtac tccctcccgg taccggtgcc
    10021 ggatctctcg gctctcagca taacagtttg tccgtcaatg atttcccagt caggtgctgt
    10081 gtctccgtct cctgttccgt tgtccggttc gctgctccag acatagagct gacattctgt
    10141 actgccgcag ttgtcataga cctggtaatc aagcggaacc tgcaccatat tatggtcagg
    10201 cggccataac ggatcatagt tctccttcag gcccgacacc accggtgctt cgttgtccca
    10261 gacataaacg tcaaaactgc atgttgccgt agcgccttca gcatttgtca gaacaaatgt
    10321 atttgtcgtc attcctgcag ggaagaatga accggagggc aggccggttg tctgggtcat
    10381 tgtgacgccc tcgcctccgc aattcggggc aggcattaca tcggggtagt agatccaggc
    10441 gccgcatatc ccgggatcgg atggagcata gatgaaggac ggacatatta tcccgggaac
    10501 ctgacatgtt gttgccatga aggcgtattc tcctgtcagt ctgttgaagg tcaccgtata
    10561 ggttcctgcg ggaacctgga tgttgggtcc gtcctggtag ccatagcctg tcgggaatcc
    10621 ataatcgccc caattgacat cccattcatt ctccatcctg aatttaactt caccctggtt
    10681 gaacgggtaa tcggtaaggg tatagatgat tccgtcggtg gttgtcagat caatgtcgtc
    10741 gtaccaccat cccggcaatg catcgcccag gatgccgatg ctcggaaatg caaaattgta
    10801 ttccccggtc aggatattaa aagtgacttt atagttgcct gccgttaccg gaatgggagg
    10861 accatcagga atggcagttc cggaggggaa agtattgtta ctccagttct ctgtccagct
    10921 gtcatttttc cggaacttag cctcgccgtc ggtaaactgg tatgctgtca tcatatacgt
    10981 tataccgtca tacgttatca tattgacatc ggtattccag tcctgccatg gagggactgc
    11041 ggtgccggcg atacccaaaa aggcagagca gttgtcaaca caggtgaatg aatactccag
    11101 ggtttccctg ttgaaagtga tatcataggt tccgggattg gttaccataa tgtcggatcc
    11161 gtagagggag cctgtgcctg cgggaaactc cgtaccgccc cagtttatcc accacaagtc
    11221 atcctgcctg aatttgaccc tgccggttgc caggtaaagc tgaacagcgt aattgattcc
    11281 gtcggcagtc tgcatatcaa catcgttgtc ccagccgctc agtgaagatc ccaggatgcc
    11341 gaccgaaggt gtatagggtc ccatgtctgt aagtgacacg ttgtcaaaat acattgacac
    11401 cggaattgca ccgacctcga agctgagttt gtgatagggg aagatgagcg ttgttttaaa
    11461 gtcgattaca acagtcttcc attcggtggt ggcgtactga gtgtatttgt catatccgat
    11521 taagctattc caatttcctc ccgcctctcc aagaaacaaa ccgtagtcgc gctcagcatc
    11581 agccatgaca tcataggaaa gtcgatacat atgagtttgt tgcaggacaa atcctgactg
    11641 aatcagctgg atttcccatg cgtagtcacc cgggtatcca tctgccggga tattgtagac
    11701 gcagattccg ctttcgatat acgctgatgc agtcacataa ggcgactggt acaggtacca
    11761 ggcgttatcg ggtgatggat cacccgtgac aagcgggatt tcagctgtgg tatcgaaacc
    11821 gccgtcggtc agaagttcct gggcatttcc cggcaggaga gccatgaaca acataaccgc
    11881 ggctacctgt gtaaagatct ttgctttcat aggctttata tttttatgat tggattacca
    11941 gaatcacttt tttactggga cgagggctcc gccgtccacc agcacattcc cgtcaactgt
    12001 cagggtgctg ccgggaatat ggaaattata tccgaaagtg gacttgttat ccccgcctgc
    12061 aaagaggtta ttaccgattc cgatcgtaat catcccggca ggcatccagc aggcaagctt
    12121 gctgccaggt atgatgctca cattgggatt gatgccgatg tcaatggaag aaaactcatc
    12181 ctttctttct gcagaagcat catatagttc cctgaggcgt tcaatacctg actctgctgt
    12241 cattgagaca agcttccctt tttgaaaaat gagggtgata ttcttcacct caactcctga
    12301 cccgaagtca gaggcaataa ctattttccc ctctgcggta ccgggtacag gtgccagggc
    12361 tacctcaccg gcaggaagat aaacctgtgt ggccaggaca ccttctttga tatcctcgtc
    12421 tgacacgatt ccgtccagga ctacagtttt ccttcctgtt atctttactt tcaggtctgt
    12481 cccgttgggg ttggttatat gcacttcatt accggcttcc aatactttct tgaatgaatt
    12541 tcccgtagtt tcaagcgcct ggtaatcgac gttcaatcct ttccagaaga tgtcagacag
    12601 ttccttgaat gtaattccga attgttttgc cctggcctcc gtgggataaa gtccgttacc
    12661 gatactgacc tgcctgattt tacggctaaa agcaagatcg gttaccggtg cgtttgcctg
    12721 gcttattgcc gccaggcgct cagccggaat atctgcaagc aggaggggat cttcattgta
    12781 attaacccca atggctgcgt caatgaagca taagatttta taatcaagat caggagcgcg
    12841 taaatcatat ttaacaggca cttcagtgta aaacatcctg gtcagcctgt cgcttccgac
    12901 agtcagaagg gggtaacctc ccttttttgc cacattgatg gcgatgtctt caagcagctc
    12961 catttcacgc actccgccct ggatcaggac gaattcgcct tcctttatgt tggcgcactg
    13021 actgacaatc ttttgtgcaa gagattctgt ctcggcagat ttagtcttgt ctgtcgttac
    13081 ctgtgctttt gcaacaaagc ctggcaacat aactagcaaa gtggcaatta agataccggg
    13141 gtttttcatt gtcgggagat tttagttagt tgaacaaaat ggtttcatcc gcaatacttc
    13201 gtctgttttt cataacaaag ttctgtcaca gcagcgaagg ccattttgct aatcgtccca
    13261 aagttgtgtc tctgtgtccc ctgaattgtt tttttgctac tcgcaggaat aacctgagct
    13321 ttcccacatt tcgattgcat cctggctgca ctcccagaat gtctcatctc ccatggtgca
    13381 tttttcgcaa taaaaaattt cgcatgagtt gaatcccgga atcagcagaa agatgagtgc
    13441 aatgtagatc ctgctgagtt ttctctttgc tgtctttgtt ttcatggccg tatgtcatac
    13501 atgaagcccg gggtccctga agaccgaagg tcatatatgg cgctattagt cagtttttta
    13561 tacaacgcac agaaaatcca tagcctttat cgagatagcc tctgtaaacc ttaatgtcat
    13621 tatccctgag gtcacgtaac catgcactgc catcattgaa ctccgtagcg gtccaccaca
    13681 gacctcggta tcccatgtag tagaatgcat gcgggtctcc gatgtggcag tgtccccctg
    13741 gaagaccgtt aaagccagat tcgttggtta ctgatgtgct cgtggttttc cagtgtgcag
    13801 tgccagtctc ttttagtttt ccaccagctg tttcactttc aattccggta acgttttgtg
    13861 cattaccatc cagattcaaa atcaaggtat gccattcagc atctgttgga acatgccagc
    13921 cggaagggca tagctttttg gtgtttaccg cataccagtt gtacagagcc ccgtagcttt
    13981 ccttatataa agcggcatcc tcctcacacc aggtatatcc gggattttta atataagagt
    14041 aggtcatcaa aggtatggcg gtaccatcgt tgtacttcgt ggttttcagg ttttcagcca
    14101 tccatacctg tgaaccaatg gttaaggaag tatagatgtt cccatccttg tcttttattt
    14161 cagaagattc ttcttctttt tggcacccat tgataaaaag gagtgagata cctgttaaaa
    14221 tcaagggtag catacggatt ttagattttg ttttcattgt ggtggatatt ttggttacaa
    14281 tcaattatca gtcaaagcta aatcatgatt atttcacaga tttttccatt tgtcccaaag
    14341 attggtctaa aagtgccaaa atggcccgag attgtggctg tttggtgggg ttcacatact
    14401 tgttttaatc cctgtagagt tgtattttta acccgaagaa gttcccggtt aataccccgg
    14461 ataaatacaa caaatatggt tgcagcaaaa aagtcaggga gatttccctg gggatggata
    14521 gtactaatcc ttgttgtcgt actgctttat tcaaccaatc cgtctgaacc gcagttcacc
    14581 tcttacctga aggacaggat cagggagcag gtgaagggtg atgagaccat gtccggtgac
    14641 cttgctaggc tgctttccgg acctgccgcc tccctggccg gactgggaac cgtcaggaaa
    14701 gactatttcc tgtgttcaac atatgagtta agcttaccgg gtgaggaaca tcttttcctg
    14761 ggcattatgg atcactttgt taaacttaaa tgaacagatc aaccggtgaa tcagatgtac
    14821 tcaccggcaa tgctcaggtt tttgtgtgca tcctcaagaa gggaatgtat gggcactcca
    14881 taggggcatg cagcttcaca aaatccttta caattcctgc agtttccggc atcaggcctt
    14941 ccgagcccca ggtattggct gatggcaaat ttttcacgtg actgtgccat aaaatagtgg
    15001 ttgaaccgca taattgtatt gaccggaaca ccggcggggc agctgctctc acagatgccg
    15061 catgcgtgac ggcaataaaa gggtccgtat atatcacgca ttgaatcaac catcgactgg
    15121 cttttctcag tcagtcgcga tcctgacaaa tttatataat cagctacttc agtgaagttt
    15181 gagaaagaga tcaggacagt actcactgcc ggatggttca ggacgaaact tattgccgcc
    15241 tctttcctgg cgtttatatc tgaaagacca tgttgtatca gaaacggcat cgcctgttcc
    15301 tggctcttca ttatcttgga gtaaacgttc tgaatattcg gggggatctc cttattttcc
    15361 ttctggtagt ttgtgaccat gttcgtcaca atattgtaag ctccaccgaa gggatcggtt
    15421 ttcataagtg ttgttccgac gttctttttc tggcattgtt ccaggatttt atcacccatc
    15481 tcctgctgga catagttata aacgaacatt acaaggtcaa agcgcccgct gtcaacggct
    15541 gcgcatataa ccttatccat tgtctccggc ggttctcctg taaaacttga cccgtggcac
    15601 gaaacgccgc tgaatctcac aatgccctcc ttcttcattt gccttacagc actatggaat
    15661 actttgttct ctgttgcgat tacagtgttt gcagaccaga gcatcaatcc gtcaatatag
    15721 ggagtgtcaa gtctttccag tgtcttcttt acccttgaga caagagtctc tcgtgtatcc
    15781 ttttcggtaa cggcaatctt tgaatttata aagagtgatt ccctgggaca ggtttttatt
    15841 gcccgcccta tggtgacttc actgtttcct cctccatgcc cctcaattga atcaatgaaa
    15901 ttaattccac cggcaacagc cgctttcagg acactttcac ttgccgtgcc agggaagccg
    15961 cacccgatgt ctgacacttt gaatccggtt ctccccagag tcctgtattc ccttatttcc
    16021 ggttgcagtt gttcccgggg tgaaatctcc cggccgaaca tcgcaccaga gcctgctatg
    16081 cctactcctg ccatacaggc tgttgatttc gacaggaata tcctccggtt gacagttctc
    16141 ttatccatat cggggtttta aggaaatcaa agttatatcg ccgcggagcg gaagtcaaat
    16201 atattttccg gctaaagact gaatacacgc gtgtaacaca tacgggcagt gtttgaactc
    16261 aaactggata agggaagtag aatacccgtt ccacagcata aaagaatggt gatacatgat
    16321 tatcctgaag ctggctcaga acaacatatt tttcagtctg ccgcttgttt gtcttacagt
    16381 attaatccct tttttcagcc gggatattga aaaccactcc cccggataga tgaatctctt
    16441 cgaaggaaaa gtaactgtga tcacccaggt acagggaacg ggataactga gcctgcagct
    16501 tgatgttttc ccatcccacg cggaaagtca ctgccggctc aaacagataa tgggctttat
    16561 cattaatgtg gtcaagttct tcaatgccca tattattgat tacgcgatag taggaaaggc
    16621 ggcaggtgcg agctgatgcc ggcacccccg tacacctcga aaacaaatgc ttgacccaga
    16681 ggtgtaaaat atcctatggc tccgtcaaga tagattcctc tcccgaagtc gttgtctgat
    16741 gcttttcctc cccaggctgt cataaaatct gccatcagcc cgattttctt cccggccgga
    16801 taagcagttt gcgcctcgat ggaaaatgac tcatcacctg ctcccaaaaa cgctgaaatg
    16861 tgcagatcct ccttttcctg gaacaggggg acattctgga catttgcgac atagtaataa
    16921 tggctgcatc cacaatggac tattgcggta aggattgcgc ccaacagtat tttgttcatc
    16981 ctgttcgttt tttcccgtgt ggtaagtttt tttatcctga accccacggc agttatgtta
    17041 aagctcggct attcagccgg aacctgcttt gctattcgtc tcaaagattg ttctctttgt
    17101 tccaaaatgc gaaaaacagt acctcactgt gcatgtaaat tcattagatc ttcatctgca
    17161 ggttccttta ttacatcggt aataatgtta aaccggtgaa ccagcctgac cagaaaatat
    17221 ttcaaaagac tcattcatct gattatatga atcttaacgt atccgttggt aagatatctg
    17281 acaggatgcc ggaggacttt ataaactacc tttcgagtgt aggtcttgtg attgaatcag
    17341 gcttgcttgt cctccctcac aagaatcatt atttctacga ttttgaagag ctgaagaggg
    17401 tgaaaacgat tgttaacctg atgaaactta atcaggtcag ggagctgagg ggctttgtac
    17461 gtaaactatc ggagttgttg cattaccagg ccaatttcgt cgggtgtttt attgacaaca
    17521 aaatctacaa tcctttttca tttaaataca gacgccttac tgaagatccc tcagaattac
    17581 cagaattcta tgaaactgga atagagtcaa ggataccttt tgtcaacagg atatataatt
    17641 tcattgatgc aaagaccgac agatctcttt caaggaaaac cgttaccaat ctcctgaatg
    17701 aatttggctt tgaggttgta ataatgacag agcagaacgg ggtgacctac tttcattcga
    17761 gaaaagtcgg ccatgagcca ctgcaattat cgcctgaaaa gttgcattcc ttgaaaagga
    17821 aggaaattcc ttcatgatga caaattccca tcctgtaaac aggggaaaga aatacagtga
    17881 tttcgggaaa atgaagtgtc tctccgtttc tccagtcata gtacatacgc cggacaatct
    17941 gaccacattg tgccggcaat ttacacattt tatatttgcc tatagatcaa tcaattaatg
    18001 tcggctcctg gtaatatgtg ttgtccggtg gtttccttat caaagtaact atactcaatt
    18061 tcccctatta taaactcacc aacaagcccg cccacacctt gacttccggg tgatgaaacc
    18121 tcctggtttg gtgtgccagt ctggtggacc ggcgcgcaaa aaccagacga agccaatgac
    18181 atataccgac ctgttatgct gcgtgattgt tacttatcgt agtgatatcg gcagctatag
    18241 ataagtaatt catcttcatt tacctgataa actaatctat gttcatggtc gatacgtctt
    18301 gaccataacc ctgccagatc atattttaaa ggttcaggtt tgccaattcc agaatgaggt
    18361 gttgattgaa tgtcttttat taaagtgtta attttcttca gaatctttct atcctctgct
    18421 tgccaggaca gataatcttc ccaggcattt ttggaaaagg taatcctctt cattcttcaa
    18481 tgagattgcc ttggaaagtt tttccagatt gcatctgttc aattgattcg tagagtcttt
    18541 gagcgttctt ttttgaactc agcaggtgta atgtttccat aatagaatta tactcctcca
    18601 gggagatcaa tacagcacct tttcctgaac cacgtttgat tatcagggtt tcattgttaa
    18661 gttccacact gtcaaggtag tccttaagct cttttctgaa ttctgaatag tttgctgcaa
    18721 tcatacttga attattaaga tcttgttaga gtacaaatat acgtacttag atacgtaatt
    18781 acaaatttcg tgcagaatat ttgttcatgc agcgtaactt atttatatgc ttaccaaaga
    18841 taagccaaat acacctaata tcaatgataa tgtattattt aggatacagc tgctcgcctt
    18901 atttgaaatt tccgattagt agaacttata ccggctgttc atttccatag tctaatttcc
    18961 ttcgataaga acaaagtcat ctacgagcaa aggcagtaga gaatagtttt aattgagaac
    19021 ataagtgttt tccaaagaat aaaagaaaaa gttaccagta tccgagggct gaatttcttg
    19081 tatcgtagca tttttctaat tattcccagg ggagacagcc ttgacaagtg aaaaggcttc
    19141 atcccaggtt actggatcat aaacatattc ataaaaaatg atgtagtggg ttaccatgtt
    19201 tccttttgca ttgatatgac ctaccccgac ataaggtaca gatgtaagcg ggatcgaaat
    19261 gcagtccgat ataaaaaaac ctcaccagtt ccactttccc aatctaatcc tacactttca
    19321 acttcccctt gaaagactct tttttccaaa ccggaatgcc gtctttatag tggtacaatg
    19381 agtggtaggt tactaatcca accagccaat cgatttg
//
LOCUS       DAOPXU010000047        19321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_2960_length_19321_cov_11.717170, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000047 DAOPXU010000000
VERSION     DAOPXU010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19321
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2960_length_19321_cov_11.717170"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PKK03_06430"
     CDS             complement(<1..236)
                     /locus_tag="PKK03_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HNX84073.1"
                     /translation="MNIIDGKKTAAEIRSEIAEAVKEIKNSGHRVPHLAAILVGNDGG
                     SETYVSHKVKDCEEVGFRSTLIRMPATVSEDVLLG"
     gene            complement(401..1723)
                     /gene="ffh"
                     /locus_tag="PKK03_06435"
     CDS             complement(401..1723)
                     /gene="ffh"
                     /locus_tag="PKK03_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNX84074.1"
                     /translation="MFESLSERLERSFKILKGEGVISEINVSETLKDVRRALLDADVN
                     FKIAKQFTDTVKQKAMGQNVLKSVKPSQMMVKIVHDELVELMGGEKSDINIKANPSIV
                     LIAGLQGSGKTTFSAKLAKLMKSKRGKHPLLVAGDVYRPAAIEQLKVLGSQIEVPVYT
                     EDGSRNPVEIARNAIREAKKNGNDLVIIDTAGRLAVDEEMMNEIAAVKNAVTPQETLF
                     VVDSMTGQDAVNTAFEFNVRIDYDGVVLTKLDGDTRGGAALSIRSVVNKPVKFVSTGE
                     KMDAIDIFYPERMADRILGMGDIISLVEKAQEQYDEAEARKLQKKIAKDQFDFNDFIS
                     QIQQIKKMGNMKDLMGMIPGVSKAIKNLDIDDNAFKGIEAIISSMTPAERSNPVLLNG
                     SRRKRVAEGSGTTVMEVNRLLKQFDETRKIMKMVAKGGDLSKMAVKRR"
     gene            complement(1743..3530)
                     /gene="argS"
                     /locus_tag="PKK03_06440"
     CDS             complement(1743..3530)
                     /gene="argS"
                     /locus_tag="PKK03_06440"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNX84075.1"
                     /translation="METLLRTGIKEAVRTLYGVVADDDLLQIQKTRKEFEGDFTLVVF
                     PLTKTSRRSPEQTAREIGEYLTEKIDAVSGYNVIRGFLNLTISPEEWGRRLAEMHADT
                     GYGFSQPGQEAETVVIEFSSPNTNKPLHLGHIRNNLLGFSLSRIIGAAGNRVIRTNIV
                     NDRGIHICKSMIAWQKYGNGRTPVSEGMKGDHFVGDYYVLFEKKLKEQAANLVTSGST
                     PEEARENAPLMQEARRLLLKWEAGDPETVNLWQMMNSWVYEGFDVTYRALGVEFDKIY
                     YESDTYKVGRDIVLRSLEQGHLIRREDGSVWIDLTADRLDQKLLLRSDGTAVYMTQDL
                     GTAVIRYNDYHFDRHIYVVGNEQNYHFQVLTLALQRMGFDWARGLHHFSYGMVELPEG
                     RMKSREGTVVDADDLIEEMTATASVMSQELGKLGGYSQDEKDNICRQIGLGALKYFIL
                     KVDPKKNMTFNPRESIDFNGNTGPFIQYTYARIRSVLKKAEETGVADTADAVEGVIPN
                     TKEISVIRLLHDFRQIVPEAARELSPALIANYCYDLAREFNQFYHDYSILGEEVKNIR
                     NFRLRLSELTGKVLEKGLYLLGIEAPERM"
     gene            3736..4194
                     /locus_tag="PKK03_06445"
     CDS             3736..4194
                     /locus_tag="PKK03_06445"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaC family regulatory phosphoprotein"
                     /protein_id="HNX84076.1"
                     /translation="MVKLQDIRIEATSKTPQVSFTATTGNLTFTGKSLPENASGFFEP
                     LYKWASEYAKNPAESTNLKFNVDYFNTSSVIWMGKILKVLTKIRKSDHILFVHLYFDI
                     EEYDSMGEEDVRESLSPFLDVTADATCSVGIRLYGVDEDGNTLKENIVLI"
     gene            4303..5751
                     /gene="amrB"
                     /locus_tag="PKK03_06450"
     CDS             4303..5751
                     /gene="amrB"
                     /locus_tag="PKK03_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNX84077.1"
                     /translation="MNIFGQEHDRQPVVSGSFYPAGSERLRSELAGYFASFRKPDAGV
                     RVRAIIVPHAGYVYSGHTAAAGFAAIPAEAVYDNIFLIGVSHRYAFEGAAVFSSGNLI
                     TPLGTLKVNREIGEKLRSTNRWFIEKDVAHGPEHSLEVQLPFIQYHFTKPPAIVPILI
                     GTRNTTVLKAIASGLQPWFNDRNLFVISSDFSHYPAYDDAKRVDRLSSDAIVKGNAEA
                     FLRTIRNIETSGTDNLATAMCGWPAGAVLLYMAEKDEGLQFRNVEYTNSGDARVGSKD
                     EVVGYNAIVLEKKSPAPEPFTLTAAEKKILLGTAREAITARLNNRKPVPVDEGRLTPG
                     LREPLGAFVTLTREGELRGCIGRFTSTDPLWEVVAAMATEAAFGDPRFPALTKAEYPS
                     VHLEISVLGPMRKINNINEIKIGRHGVYIKKGFRSGTLLPQVATERGWTVEQFLGYCA
                     RDKAGIGWDGWKEKDTEIFVYEAYVFGEQEQK"
     gene            5748..8189
                     /locus_tag="PKK03_06455"
     CDS             5748..8189
                     /locus_tag="PKK03_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84078.1"
                     /translation="MTYHVVPIGVTVLVIYLFSLYLSSSGFTARQSHRRFWNWILLCS
                     FLIAALLGIFLALRITYRWEVSFAESLMHWHVEAGIAMAFASLIHLSWHLGYYFRRLR
                     IRENDVAHAAAPPATGMDAAGVDAAGLRPLLLLTGFVSSASQFILLREAAILSGGTEA
                     MTGLFLWLWLIIAAGGAVTGSRSVITSVRRMVWTLIAGTALAPLCFLMMNQIILSPGQ
                     TPSFSQALVILAVSVAPVTFISALIFVRISVLRNAAGIARAGNSFAAETAGSVAAGVV
                     TALTVTLRIPNYQLYMVILTVAVACAAWFLGYPRRMKIAVPAAMAVIAGLLFIFPPDA
                     AIRSILLRGVRAERSIDTPFGNITTGVYSNERTVYYDHRPVFFPGDVISAEENIHYAL
                     LQRQNYDRVLLISGGLRRHLAELMKYNIRELTYLEMDPGLIAAEGVHDTVCGQMRVKV
                     VRDDPAAFLRADGGAFDAVIQLIPPPSTLSVDRFYTVEYFRIVREQLSPEGVFICTPM
                     PWFNYSPESYRRGFSPFFNALSAVFSHVTLIPGSMLYAVASGTPVSHAVVQLAGQRGI
                     GASYVNGDYLNDNEIKTKTEQILSNVDRNAGMNTALRPVTSLFSNLLSLERMGMRGGI
                     IALLGLLILLPFAFVSRGGQVMFASSAGLAGFGMILIFVLQMTAGNIYMLSSVILTLL
                     MAGLAAGAALGERLALKSLKICILILTGIFTLTGLLVPALTVSSSGPVLTFIFIVLPA
                     AGIVTGAIYRILTSRPGGRVTGSVYSADMAGSALGYLTAATLLVPLAGTANGCFILAL
                     FILLSGIVASVTIKA"
     gene            8221..9339
                     /gene="amrS"
                     /locus_tag="PKK03_06460"
     CDS             8221..9339
                     /gene="amrS"
                     /locus_tag="PKK03_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNX84079.1"
                     /translation="MKDNDNNISRRSFARRGSLALAAGFFAPFPAGAMRPAPVERVAM
                     FQEAGPKGVVCLICPNECTLKDGELSDCHNRIARKSKLYTMAFGNPCAVNTDPVEKKP
                     LYHFLPGTTAFSIATAGCNLACLNCQNWTISQTSPDKTRNFDLLPPDVVAQAAAGGCR
                     SIAYTYSEPVTFYEYVYETSRLAREKGIKNIMVSNGYINREPLRQLCRYVDAANIDLK
                     SFSDSTYLKLTGARLQPVLDSLKTYRDEGVWLEITNLVVPGWTDKPDEIRQMCDWLAE
                     KGFTDTPLHFSRFQPQYKLEHLPPTPAGILTKAAETARAAGIKYVYIGNLPGAGNDDT
                     ICPSCGKKVVDRSGFRIVSQALSGGRCACGTKIPGVWS"
     gene            9371..11377
                     /gene="ligA"
                     /locus_tag="PKK03_06465"
     CDS             9371..11377
                     /gene="ligA"
                     /locus_tag="PKK03_06465"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNX84080.1"
                     /translation="MKLSDAKLRVEELRKTIDEHNHRYYVLNQPVITDFEFDLLLNEL
                     QTLEKNFPELVTPDSPTMRVGSDLTDEFVQVQHRWPMMSLGNTYSEEELTEFGERVKR
                     TLGQEPEYVCELKYDGVSISLTYENGVLSGAVTRGDGMVGDDVTVNVRTIRSIPVRIN
                     APGAPSSFVVRGEIYLPLKGFNEMNAARMEKGEAPFANPRNAAAGTLKLLDPRMVAAR
                     PLDCFLYFLLGDSLPAGNHYDNIMAARSWGFRTPDVMERCSGMEEVISFIRKWETARK
                     QLPYDTDGVVVKVNSLEAQRLLGSTAKSPRWAIAYKYAAEQAVTELLSVDFQVGRTGN
                     VTPVANLRPVQLAGTTVRRASLHNSDQIELLGLHYGDSVIIEKGGEIIPKIVGVDAGK
                     RAGDAAVVTFPEKCPECSTPLVRNEGEANHYCPNYLHCPPQITRRIIHFVSRKAMDIE
                     GLGEETVELLWSNGLARNVADLYDLKHEQLASLDRMGDKSASNILASLAASLSAPWNR
                     KLFALGIRHVGETVAKTLAGAFPDIGRLMDATEEELLALPDIGPRISASVREYFSDED
                     NREIIRRLKAAGMTFEGAVQTRPSAGPLAGKTIVVSGTFEQYTREGIKAAIEAAGGKN
                     AGSVSGNTSFIVAGSDMGQAKRAKAASLGIPVITEEEFVKMIKE"
     gene            11466..11993
                     /locus_tag="PKK03_06470"
     CDS             11466..11993
                     /locus_tag="PKK03_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84081.1"
                     /translation="MMLYRRLAALRRMKQDFNLDRVRKIGILWDASFENDFQYLAALN
                     RQLSETGKSVEVIAWIPGKIVPDRLTGLSYMKFLRKTDLNWAFFPVSDDAKKFLETKY
                     DLLIDVNPSSLFQLSALAALSAAPMKVGPDLTDEPENTPYDLMIRAPKPFSIAFFIDQ
                     AMHYLSLIGSPETRA"
     gene            complement(12023..15343)
                     /gene="secA"
                     /locus_tag="PKK03_06475"
     CDS             complement(12023..15343)
                     /gene="secA"
                     /locus_tag="PKK03_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNX84082.1"
                     /translation="MSIINKVLSVFLGNKEERDLKEINPFVDKIQKESALLSSLSNDA
                     LRDRTAALKQEIRDHISAEEKEIAALRVSAENEEDVNRKEELYNQVDKLAKDVLEKIE
                     NKLDEILPVAFAIVKETAHRFKENDVLEVTARDWDRNLAATRPSITVEGEKAYWKNRW
                     MAGGNEIVWDMVHYDVQLIGGVALHKGKIAEMATGEGKTLVATLPVFLNALAGKGVHI
                     VTVNDYLSKRDSEWMGPLYEFHGLSVDCIDKHEPNSHARRKAYNSDITFGTNNEFGFD
                     YLRDNMAINPEDLVQRKHHYAIVDEVDSVLIDDARTPLIISGPTAKSDTSQFDEYKFK
                     VEKLVGAQRKLVTQLLADSKRLIAEGDNEKGGLLLLRAYKGLPKNSALIKYLSEEGVR
                     SLLQKTENFYMQNNSKEMPKVTEELFFVIDEKANSIELTEKGHDLISTSVEDSSFFVL
                     PDVGSKIADIEKSVRDENERRKAKDELLQDFAVKSERVHTMTQLMKAYTLFEKDVEYV
                     VMDNKVKIVDEQTGRILEGRRYSDGLHQAIEAKENVKVEASTQTYATITLQNYFRMYH
                     KLAGMTGTAITEAGEFWNIYKLDVVVIPTNVAVIRNDHEDLVYKTKREKYNAVIDEIV
                     KLNKAGRPVLVGTTSVEISELLSRMLKMKGLKHNVLNAKLHQREAEIVAEAGKTGTVT
                     IATNMAGRGTDIKLTPEVKKAGGLAIIGTERHESRRVDRQLRGRAGRQGDPGSSQFFV
                     SLEDDLMRLFGSERISGIMDKLGLKDGEMIQHPMITKSIERAQKKVEENNFGIRKRLL
                     EYDDVMNSQREVIYSKRRHALLGERLSVDIANMMFDVTENIVNTYKDISDFEEFQFAL
                     IRSLSIDSPVAKADFSKDSSNEVVDVVYDKVLEAYRRKSEIISATAYPVLKDVYERMS
                     ATYENVVVPISDGARTYNVITNLKAAVESQGRELLRSFEKTSVLATIDDAWREHLREM
                     DDLKQSVQNATYEQKDPLLIYKFESFELFKTMINKVNQDVVSLLMKGTIPAAASTDNV
                     REAQRRKQADMSRLRTQKSDYDSYRSGGGGSPEQKQKQMAPVRVEKKVGRNDPCPCGS
                     GKKYKHCHGQGLADSAGA"
     gene            complement(15527..16015)
                     /locus_tag="PKK03_06480"
     CDS             complement(15527..16015)
                     /locus_tag="PKK03_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNX84083.1"
                     /translation="MLTKKVEELCNRQIEREGYSSNLYLAMASWAETKGMSGIAAWLY
                     AQADEERAHMLKFFRYVNERNGSAIVPEFRKPPVKYKGIPEMFDEVLKHEKFISASIN
                     EVLEAAIADKDHATANWIQWFVKEQVEEESSAQQIIDKLNLAGQHGVYFLDRDIMNLR
                     GK"
     gene            complement(16073..16438)
                     /locus_tag="PKK03_06485"
     CDS             complement(16073..16438)
                     /locus_tag="PKK03_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX84084.1"
                     /translation="MAGVMIVEDDSALRELLRDALEKRKYTVIAAADGREALAKFRPS
                     VVDLVITDLLMPEEDGLMVIMKIREIKPSTRMIAISGGGMAGPGSYLMMASKLGADAV
                     ISKPFLPSELVMKVKELLE"
     gene            complement(16471..17253)
                     /locus_tag="PKK03_06490"
     CDS             complement(16471..17253)
                     /locus_tag="PKK03_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX84085.1"
                     /translation="MSTRKDKDQADNSQKTPGSDPGAGSRLGALAGGIAHDLNTVITT
                     IYGYSEMALESLTGSPEAAGNIRKIIGAADRARLLTGQLLDLSRHAAREKVPVRVAAV
                     LADTIDFIRPSVPEGIRIMRRVTAPDAYVNAVPAQLFRIFLNLTMNAIQAMTGKGGTL
                     TVTLGCRENVKPIHHDGECRELHIRFSDTGKGMDEALQREIFKPFVSAGKEHGTGLGL
                     TVVADAIREMGGTITVSSEPGAGTAFDIIIPGAFFGTLPEKH"
     gene            complement(17271..19016)
                     /locus_tag="PKK03_06495"
     CDS             complement(17271..19016)
                     /locus_tag="PKK03_06495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNX84086.1"
                     /translation="MDNFIVSARKYRPATFDMVVGQDTITTTLRNAIRNNLLAQAYLF
                     CGPRGVGKTTCARIFAKTINCSDTGPDGEACDNCESCKSFNSQASFNIHELDAASNNS
                     VDDIRNLTDQVRIPPQIGRFSVYIIDEVHMLSTQAFNAFLKTLEEPPSHAIFILATTE
                     KHKIIPTILSRCQIFDFNRINVDDIVQRLSWVAGNEGVNAEHEALHVIATKADGAMRD
                     ALSIFDQIVSFSGKTITYADVIANLNVLDYEYYFRITAAAMDGDVTSVLLTYSEILNK
                     GFDGYNFMAGLNNHLRDLLVSRDEATVKLLETSPSIRQRYREQAVKAPLQFIYEALDI
                     GSQCMLNFRASKNQRLHVELALLRMCNLSAAGAQEEVKKKAEPGRLEEEEDFSVAPPR
                     SGASPQTTPVRTPPEASPSPGPAIQRPKPPATGDEPKKVSIKNIIAGAKSPEAIVSEP
                     QPDTTLISHDLEFTAEELREAWASFAEEIKEESPRISVTLSSVTPELLADKTVLLKLD
                     NLTLKETFDHNFKPRLEGHLRRTLRNGSLRLQTTVEATERGEILYSPEQKFNHLAAKN
                     PALRDLKKTFNLDFE"
     gene            19152..>19321
                     /gene="rpsF"
                     /locus_tag="PKK03_06500"
     CDS             19152..>19321
                     /gene="rpsF"
                     /locus_tag="PKK03_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNX84087.1"
                     /translation="MNQYETVFIATPVLSENQMKEAVQKFKKIITDNEGEIIHEENWG
                     LKKLAYPIQKKST"
BASE COUNT         4227 a         5048 c         5029 g         5017 t
ORIGIN      
        1 ccgagcagaa catcctcact cacggttgcg ggcatcctga tgagtgtgga cctgaagccc
       61 acctcctcgc agtccttcac cttgtgggag acgtaggttt cgctgccgcc gtcgttaccc
      121 acgaggatgg ctgccagatg gggaacgcga tggcctgaat tttttatctc tttcaccgct
      181 tcggcgattt cacttctgat ttctgcagct gtttttttcc cgtcaatgat gttcataatt
      241 gcgattcaga atttggggta tgtggtttta tgcgtcaatt acaatgcctg atcccctgtg
      301 gggattgatt ctcatcatgt ctctggtcta caatgcttta tcccctgcgg gaatttcgtc
      361 aatcgcaaat cgcaaatcgc aaatcgcaaa tctacaatgt tcaccgtctc ttcactgcca
      421 tcttggacag gtcacctcct ttggcaacca tcttcatgat cttgcgtgtc tcatcaaact
      481 gtttcagaag ccggttcacc tccatgacag ttgtgccgct cccttcggcg actctcttgc
      541 ggcggctgcc gttgagcagc accgggttcg atctctcggc cggagtcata ctggagatga
      601 ttgcttctat tcccttgaaa gcattatcat caatgtcaag gtttttaata gccttgctta
      661 ccccggggat cattcccatc aggtccttca tgttccccat cttcttgatc tgctgtatct
      721 gagagatgaa gtcgttgaag tcgaactgat ccttggcaat cttcttctgc agcttgcgtg
      781 cctcagcctc gtcgtattgt tcctgtgcct tctcgaccag gctgatgata tcgcccatgc
      841 ccagtatcct gtcagccatc ctttcagggt agaagatgtc aatggcatcc atcttttcgc
      901 cggtgctgac gaacttgaca ggtttgttca ccactgatct tattgaaagg gccgcaccac
      961 ccctggtgtc gccgtcgagc ttggtgagaa ccaccccgtc gtagtctatt ctcacgttga
     1021 attcaaaagc cgtgttgacg gcatcctggc ctgtcatcga gtcaaccacg aagagagtct
     1081 cctgaggtgt gacagcgttc ttcacggccg ctatctcgtt catcatctcc tcatccacag
     1141 caaggcgtcc ggcggtgtct atgattacca ggtcattgcc attcttcttc gcctccctga
     1201 tggcattcct ggctatctcg acaggattgc ggctgccatc ttcggtatat accggaacct
     1261 ctatctggga accgagcacc ttcagctgct caattgcagc aggcctgtag acgtcgccgg
     1321 ccaccagcag cggatgtttg cccctcttgc tcttcatgag cttggccagt ttggcgctga
     1381 aggttgtctt gccgcttccc tgcagtccgg ctatcagtac tatcgaagga tttgccttga
     1441 tgttaatgtc gctcttctcc cctcccatca gttccaccag ctcatcatgc acaatcttga
     1501 ccatcatctg cgacggtttg actgacttga gcacgttctg acccatcgcc ttttgcttga
     1561 cggtgtcagt aaactgtttg gctatcttga agttaacgtc ggcatcaaga agcgctctgc
     1621 gcacatcctt gagggtttca gaaacattga tttcagagat caccccttct cccttcagaa
     1681 tcttgaagga gcgttccagt ctttcactca ggctttcaaa cataattaat gataatctag
     1741 ttttacattc tttcaggggc ttcgataccc agcaggtaga gccctttctc cagcaccttt
     1801 ccggtcagtt ccgacagccg gaggcggaag ttgcgtatat ttttaacctc ttcgccaagg
     1861 atagagtagt catggtaaaa ctggttgaat tcccttgcca gatcataaca gtagttggca
     1921 attagggcag gactcagttc ccgtgctgcc tcaggcacaa tctgccggaa atcgtgcagc
     1981 agtcttatca cactgatctc cttggtattt ggaattactc cttcaaccgc atcagcagta
     2041 tctgcgaccc cggtttcctc tgcctttttc aggactgacc ttatcctagc ataggtatat
     2101 tgtatgaacg ggccggtatt gccgttgaaa tcgattgact cccgcgggtt gaaggtcatg
     2161 ttcttcttcg ggtctacttt caggatgaag tacttcaggg ctcccagtcc gatctgcctg
     2221 caaatattat ctttctcatc ctggctgtaa ccgcccagtt tgccaagttc ctgtgacatc
     2281 acgctggccg tggcagtcat ctcctcaatg aggtcatcag cgtcaaccac tgtaccttct
     2341 cgtgacttca tcctgccttc gggaagctca accataccgt aggagaagtg gtgcagtccc
     2401 cgtgcccaat cgaatcccat cctctgcagc gccagtgtca gcacctggaa gtgatagttc
     2461 tgttcattgc ccaccacgta gatatggcgg tcaaagtggt aatcattata ccttattact
     2521 gcggtgccca ggtcctgagt catatagacc gcggtaccgt cagaccggag caggagcttc
     2581 tggtcgagcc tgtctgcagt gaggtcgatc cagactgaac cgtcttccct gcggatcagg
     2641 tgtccctgtt caagggagcg gagcactata tccctgccga ccttgtaggt atctgactca
     2701 tagtagattt tgtcaaactc cacacccagt gccctgtatg tcacgtcgaa gccttcgtag
     2761 acccaggagt tcatcatctg ccacagattc accgtttccg ggtcacctgc ctcccattta
     2821 agaagcaggc ggcgggcttc ctgcatcagc ggtgcatttt cgcgtgcttc ctcgggggtg
     2881 ctccctgacg tcacaaggtt tgctgcctgt tctttcagtt tcttttcaaa aaggacataa
     2941 tagtcaccca cgaaatggtc gcctttcatc ccttcggaga cgggagtgcg gccattgccg
     3001 tacttctgcc aggcgatcat tgatttgcag atatggatgc cgcggtcatt gacgatgttg
     3061 gttcttatga ccctgtttcc tgcagcgcca attattctcg agagggagaa accgagcagg
     3121 ttgttgcgta tatggcccag gtgcagcggt ttattggtat tcggcgatga gaattcgatg
     3181 accacggttt cggcttcctg cccgggctgt gaaaacccgt agccggtgtc agcgtgcatt
     3241 tcagcaagcc gtctgcccca ttcttccggt gagatggtca ggttcaggaa tcccctgatc
     3301 acattgtagc cgctaacggc gtcgatcttc tccgtgaggt attcgcctat ctcccttgcg
     3361 gtttgttcag gtgagcggcg ggaagtttta gtaagcggga agaccacaag ggtgaagtcg
     3421 ccctcaaatt ccttccgggt cttctggatc tgcaggagat catcatcagc cacgacaccg
     3481 taaagtgttc tgacagcttc ttttatcccg gttctgagca acgtttccat cctgtttcag
     3541 ttatggctgc aaatatagca taatagtttt ttacagcgtc ctgtgattgt tcagcggatg
     3601 atcataaatt gttcaaaaaa gttgataact gttagttatt ttttgacgaa gacctcaaca
     3661 tcatcaaaaa aaacaggtta cctttagctt gtacgttggg gcaattgtac aatactaggg
     3721 aaggaatcag atcttatggt aaaattacag gatattcgaa ttgaggctac ctctaaaaca
     3781 ccgcaggtaa gttttacagc aaccaccgga aatctaacat ttacaggcaa atcgttgcct
     3841 gaaaacgctt caggtttctt tgagccgcta tacaaatggg cgtcagaata tgccaagaat
     3901 ccggctgaga gcacgaatct aaaattcaac gttgattact tcaatacctc ctctgtcatc
     3961 tggatgggga agattctgaa agtactgacg aaaatcagga agagtgatca tatactgttt
     4021 gtgcatctct attttgacat cgaggagtat gattcaatgg gtgaagaaga tgtaagggaa
     4081 tctctctccc cgttcctgga tgtaaccgct gatgccacct gcagtgtagg tatcaggctc
     4141 tacggggttg atgaagacgg aaatacactg aaagagaata tcgttctcat ctgacagggt
     4201 cagtctcggt ttttaccggt ttttggttta actttacggg atgcaactgc ttttgctatg
     4261 aaaagggtta gtatcatcct ggtaatgtta atttttccgc taatgaacat attcggacag
     4321 gaacatgaca ggcagccggt tgtctcaggg agtttctatc ccgccggcag cgagaggctc
     4381 aggagtgaac ttgcagggta ttttgcatca ttcaggaagc ctgatgccgg agtgagggtg
     4441 agagccataa tcgtacccca tgccggctat gtctattcag ggcacactgc tgcggcaggt
     4501 tttgccgcca tacctgcgga agctgtatat gacaacattt ttctgatcgg cgtcagtcac
     4561 cgctatgcct ttgagggtgc cgcggtattc agctccggga acctgattac tccgctcgga
     4621 actcttaaag tcaacaggga gattggtgaa aagctcaggt caaccaacag gtggttcatc
     4681 gaaaaagatg tggctcacgg tcctgagcat tcgctcgagg tgcaactgcc cttcattcag
     4741 tatcatttca ctaagccccc ggccatcgtt ccgatactga tcggcaccag gaacaccact
     4801 gtacttaagg cgatagcctc aggacttcag ccgtggttca atgaccgcaa cctttttgta
     4861 atcagcagtg atttttcgca ttatccggcc tatgatgacg caaagcgtgt cgacaggctt
     4921 tccagcgatg ccatagtcaa aggtaatgcc gaggcttttc tccggacgat aaggaacata
     4981 gagacttcgg gtactgacaa cctggccacg gccatgtgcg ggtggcccgc aggggcggta
     5041 ctgctctata tggctgaaaa ggatgaggga ctgcagttcc gcaacgtgga atacaccaac
     5101 tccggtgatg ccagggttgg ctcgaaggat gaggttgtgg gttacaatgc aattgtactg
     5161 gagaagaaga gtcctgcacc tgaacccttc acgctgacgg cggcagagaa aaagatcctg
     5221 ctcggcacag cgcgggaggc cattacagcg agactgaaca atcgcaaacc tgttccggtg
     5281 gatgaaggca ggctgacacc ggggctcagg gaaccgttag gagcattcgt gacactgacc
     5341 agagagggtg agctcagggg atgcatcgga cggttcacct caactgatcc actgtgggaa
     5401 gtggtagctg ccatggccac ggaagctgcc ttcggcgacc ccaggttccc tgcgctgacg
     5461 aaagctgaat acccttccgt tcacctggag atttcggttc tcggaccaat gaggaaaata
     5521 aacaatatca acgaaataaa gatcggccgg cacggcgtct atattaaaaa aggcttccgg
     5581 tcaggaaccc tgttgccgca ggtggccact gaaaggggct ggaccgtgga gcagttcctc
     5641 gggtactgtg cccgcgacaa ggcaggaatc ggctgggacg gctggaaaga gaaggacacg
     5701 gagatatttg tctatgaggc ctatgtcttc ggcgaacagg aacagaaatg acctaccatg
     5761 ttgtgcctat aggggtgacc gtcctggtca tctacctttt ttcactctac ctctcatctt
     5821 ccggtttcac cgccaggcag tcgcatcgtc gtttctggaa ctggatactg ctctgttcct
     5881 ttctcattgc tgcccttctc ggtatcttcc tggcgctgcg catcacctac cggtgggagg
     5941 tgtcgtttgc ggaatcactg atgcactggc atgtggaggc aggcatcgca atggcttttg
     6001 catctctcat tcatctctct tggcatctgg ggtattattt ccgcaggttg cgcatccgcg
     6061 aaaatgacgt agctcatgct gcggcgccgc cggccacagg aatggatgcc gcaggggttg
     6121 atgccgcagg gctcaggccg ctgctgctgc tgacgggttt tgtaagctcg gcatcgcagt
     6181 tcatactgtt gcgcgaggct gcgatactca gtggcggcac ggaagcgatg acaggcctct
     6241 ttctctggct gtggctcatc attgcggccg gcggcgccgt gaccggcagc cgttcggtca
     6301 tcacctccgt gcggaggatg gtttggacgc tgattgccgg cactgccctg gccccgctct
     6361 gcttcctgat gatgaaccag atcatcctca gtcccggtca gacaccatcc ttctcccagg
     6421 cgctggtgat actggctgtc agcgtggcac ctgtgacatt catctcggcg ctcatctttg
     6481 tccggatctc ggtgctcaga aatgcggcag gcattgcgag ggcaggaaac agcttcgccg
     6541 ccgaaacggc cggatcagtg gcggcaggcg tggtgacggc gctgacggtg accctccgaa
     6601 tacccaacta ccaactgtac atggtcatcc tgacggtggc agtggcatgc gcagcgtggt
     6661 tccttggtta cccccgcagg atgaagatcg cagtacctgc agcaatggcc gtcatagccg
     6721 gcctgctttt catcttccct cctgacgcgg caataagaag cattctcctg cgcggcgtca
     6781 gggcagaaag gagcatcgac actcccttcg ggaacatcac tacgggagtt tacagcaacg
     6841 aacgtactgt ctattatgac cacaggccgg tctttttccc cggcgacgtg atctcggccg
     6901 aggagaacat acattatgcc ctgctgcagc gccagaacta tgacagggtg ttgcttatct
     6961 caggcgggtt gaggaggcat cttgctgagc tgatgaaata caacatccgg gaactgacct
     7021 acctggaaat ggaccccggc ctcattgcgg ctgaaggggt gcatgacact gtctgcggtc
     7081 agatgagggt gaaggtggtc cgtgatgacc cggcggcctt cctgagggct gatggcggag
     7141 cctttgatgc agtaatccag ttgatacctc ccccttcgac gctgtcagtc gaccgtttct
     7201 ataccgtgga atacttccgg atagtaaggg agcagctctc gcctgaaggt gtcttcattt
     7261 gtaccccgat gccgtggttc aattattcac cggagagtta ccgaagggga ttttcaccat
     7321 tcttcaacgc actctctgcc gtattcagcc atgttacact catccctggc tcgatgctct
     7381 atgccgtggc ctccggcacc ccggtaagcc atgcagtggt gcagcttgcc ggtcagcgcg
     7441 ggatcggggc cagctatgtt aacggcgact atctgaatga caatgagata aaaacgaaga
     7501 ccgagcaaat actgtcaaat gtcgacagga atgcagggat gaatacagct ctgcggccag
     7561 tcacctccct cttctcaaac ctcctttcac ttgagaggat gggaatgagg ggaggcatta
     7621 tcgccttgct gggactgctg atattacttc cctttgcctt cgtatcaagg ggcggacagg
     7681 tcatgtttgc ttcatcagcc ggactggcag gattcggcat gatattgatc ttcgttctcc
     7741 agatgacagc ggggaacatt tacatgctgt cgtctgtcat cctgaccctt ctgatggccg
     7801 gactggcagc aggcgctgca cttggtgaac ggctggcgct gaagagcctg aagatatgca
     7861 tactcatcct gacagggata ttcaccctga caggattgct ggtgccggct cttacagtat
     7921 catcctccgg cccggtgctg acattcatct tcattgtgct gcctgcggca ggaattgtca
     7981 caggcgcaat ttacaggata ctgacatcac gccccggagg ccgcgtcaca ggcagtgtgt
     8041 actcagctga catggcaggt tctgccctcg gttacctcac tgcagcaacc ctgcttgtcc
     8101 cgctggcagg aacggcaaac ggctgtttta ttcttgcact gttcattttg ctttcaggca
     8161 tagttgcatc tgtgacgata aaagcgtaga tttacagcag gcaacagaat ccataacaga
     8221 ttgaaggaca acgacaataa tatttccagg aggagttttg ccaggagggg ttcccttgcc
     8281 cttgcagcag gcttttttgc cccctttcct gcgggcgcca tgagacctgc cccggttgag
     8341 agggttgcca tgttccagga ggctggcccg aagggtgtgg tttgcctgat ctgtcccaac
     8401 gagtgcactc tcaaggatgg ggaactgagc gactgtcaca accggattgc caggaaatca
     8461 aaactttata cgatggcttt cggcaacccg tgcgccgtaa acacagatcc ggtggaaaag
     8521 aaacccctgt accatttcct cccgggcacc accgcttttt caatagccac ggccggatgc
     8581 aacctggcat gcctcaactg ccagaactgg accatttcgc agacatcacc ggacaagaca
     8641 aggaattttg acctgctgcc cccggatgtg gtggcacagg ctgctgccgg aggatgcagg
     8701 tcaattgcct atacctattc cgagcccgta accttttacg aatacgtcta cgaaacatcc
     8761 aggctggcaa gggagaaggg aataaagaac ataatggtat ccaacggtta tatcaaccgg
     8821 gagccgctca gacagctctg ccgctatgtt gatgccgcca atatagatct taaatcattc
     8881 agtgacagca catatctgaa actcacaggt gcgcggctgc aacccgttct tgacagtctc
     8941 aaaacctacc gtgatgaggg tgtctggctg gagattacca acctcgtggt tccggggtgg
     9001 actgacaaac ccgatgagat caggcagatg tgcgactggc tggctgagaa aggattcact
     9061 gacactccgc tccatttcag ccgcttccag ccccagtaca agctggagca tcttcctccc
     9121 actcctgccg gcatactgac aaaggcggca gagacagcaa gggctgccgg aattaaatat
     9181 gtctatatag ggaatctccc cggtgccgga aatgatgaca ccatttgtcc ctcctgcgga
     9241 aagaaagtag tggacagaag cggattcaga atagtgtcac aggccctctc cggtggcaga
     9301 tgcgcatgcg gaaccaaaat ccccggagtc tggagttaaa tcatctttca aatatcttct
     9361 gaaatatcat atgaagcttt ctgacgctaa actaagggtt gaagaactaa ggaaaacaat
     9421 tgatgaacat aaccatagat attatgttct caatcaacct gttataactg actttgagtt
     9481 tgatctgctg ctaaatgagc ttcagacact cgagaaaaat ttcccggaac ttgtaactcc
     9541 tgattctccc accatgagag tcggcagtga tctcacggat gagtttgtac aggtgcagca
     9601 caggtggccg atgatgtcac tggggaacac ctacagtgaa gaggaactta ctgagtttgg
     9661 agagagggtg aagaggaccc tgggtcagga gccggaatat gtatgtgagc tcaagtatga
     9721 tggcgtatcg atcagcctga cctatgagaa cggagtcctg tcaggcgctg taacgcgcgg
     9781 tgacgggatg gttggtgatg atgtgaccgt caatgtaaga accattcgca gtatccctgt
     9841 ccggataaat gcccccggcg ccccctcctc ttttgttgtg cggggcgaga tatacctgcc
     9901 gctgaagggt ttcaatgaga tgaatgctgc ccgcatggag aagggagagg cgcctttcgc
     9961 caatccgagg aatgctgccg caggcacgct caagctgctt gatcccagaa tggtggcggc
    10021 acgtccgctc gactgcttcc tgtatttcct gctgggagac agcctccctg ccggcaatca
    10081 ttatgataat attatggcag ccaggtcatg gggtttccgt actcctgacg tcatggaacg
    10141 ctgcagcggc atggaagaag tgataagctt tatcaggaag tgggagactg cgcggaaaca
    10201 actgccttac gacactgacg gtgtggtagt gaaggttaac tcacttgaag cgcagcggct
    10261 tctggggagc actgccaaat ccccccgctg ggccatagca tacaaatatg ccgcagagca
    10321 ggctgtcaca gagttgctct cggtcgattt ccaggtgggg agaacgggta atgtcacccc
    10381 cgttgccaat cttcgtcctg tgcagcttgc cgggacaacc gtcaggcgcg cctcgctgca
    10441 taacagtgac cagatagagc tcctggggct tcactatggt gacagtgtta taattgaaaa
    10501 gggtggcgag atcatcccca agattgttgg tgttgatgcc gggaagcgtg ccggtgatgc
    10561 tgccgttgtg acgttcccgg agaaatgtcc tgaatgctcc acccccctgg tgcgcaacga
    10621 gggtgaagcc aaccactact gtcccaacta cctgcactgt ccgccgcaga tcacccggcg
    10681 tatcattcat ttcgtctcac gcaaggcgat ggatattgaa gggctcggag aggagactgt
    10741 tgaactgctc tggtcaaatg gccttgcacg caatgtggcc gacctgtatg atcttaaaca
    10801 tgaacagctt gcatcacttg acaggatggg tgacaaatca gcttccaaca tccttgcgtc
    10861 acttgccgcc tccctttccg ccccgtggaa caggaaactt tttgcgctgg gcataaggca
    10921 cgtaggcgaa actgtggcta aaacacttgc cggagcattc cctgatatcg ggaggcttat
    10981 ggacgcgaca gaagaggaac tgctggcact tcccgatata ggtccgcgta tcagtgccag
    11041 cgtacgtgag tatttctctg atgaagacaa cagagagata ataaggaggc tgaaagcggc
    11101 agggatgacc ttcgagggag ccgttcagac cagaccatct gcgggaccgc tggccggcaa
    11161 aacaatagtg gtgagcggta cctttgaaca gtatacccgt gagggcatca aggcagccat
    11221 tgaagccgcg ggaggtaaaa atgcggggtc tgtctcggga aacacctcct tcatcgtggc
    11281 cggaagcgac atgggacagg caaaaagggc aaaagccgca tcgctgggca ttcctgtaat
    11341 cacagaggag gagtttgtca aaatgattaa agaataatac acccgttaat cctgataagc
    11401 caaaaaatct ataggtttgc attcttatgg gactctttgt caatttaagg ttaaaagccg
    11461 gcaggatgat gctttacaga cgccttgcgg cactgagaag gatgaagcag gatttcaacc
    11521 ttgacagggt caggaagata ggaatactgt gggatgcctc ctttgaaaat gatttccagt
    11581 atcttgcagc tctcaacagg cagctttctg agacagggaa gagcgttgag gtgattgcct
    11641 ggatccctgg aaaaattgtt cctgacaggc ttaccggtct gtcatacatg aagttcctga
    11701 ggaagaccga tctcaactgg gccttcttcc cggtatctga tgacgcaaaa aaatttctcg
    11761 agaccaaata tgacctgctt atagatgtca atccctcttc actctttcag ctcagtgctt
    11821 tggccgcact ctcagcagca cctatgaagg tgggtccgga tctgaccgat gagccggaga
    11881 atacaccgta tgatcttatg ataagggcgc cgaagccctt cagcattgca tttttcattg
    11941 atcaggcaat gcattacctg tcacttatcg ggagcccgga gacacgggca taatcagcag
    12001 tgccaggcgc aacagcacag gtctatgcgc ctgccgaatc agcaaggccc tgaccgtgac
    12061 agtgcttgta tttctttccg ctgccgcacg ggcacgggtc attcctgccg actttctttt
    12121 ccactctcac aggcgccatc tgcttctgtt tctgttcggg cgagccgccc ccgccgctgc
    12181 ggtagctgtc ataatcgctc ttctgggtcc tcaacctgct catgtctgcc tgtttgcggc
    12241 gctgggcctc ccgtacatta tccgttgagg cggctgcagg gatcgtgccc ttcatcagga
    12301 ggctgaccac atcctgattc accttgttaa tcatagtctt gaagagctcg aacgattcaa
    12361 acttatagat aagcagcggg tccttctgtt cgtaagtggc attctggacc gactgtttga
    12421 gatcatccat ctccctcagg tgttcgcgcc atgcgtcgtc aatagtggcc agtacggaag
    12481 ttttctcgaa tgaacgcaac agttccctgc cctggctctc cacggcagcc ttcaggttgg
    12541 tgatgacatt gtaggtacgg gctccgtcag agatggggac aaccacgttc tcataagtgg
    12601 ctgacatccg ctcatagacg tctttcagca caggataggc cgttgccgag atgatctcgc
    12661 tcttgcgccg gtaggcctca agcaccttgt catagacaac atccaccact tcattgctgc
    12721 tgtccttgct gaaatcagcc ttggcaaccg gcgagtcaat tgaaagagac ctgatcaggg
    12781 caaactgaaa ctcctcaaaa tcagatatat ccttataagt attaacaata ttctctgtaa
    12841 cgtcaaacat catgtttgca atgtcaacac tgagtctttc gccaagaagt gcatgacggc
    12901 gcttgctgta aatgacctcc ctctgtgagt tcatcacatc gtcatactcc agcagcctct
    12961 tcctgatgcc gaagttgttc tcctcgacct ttttctgggc tctttctatc gacttggtga
    13021 tcatgggatg ctgtatcatc tctccgtcct tcaggcccag cttgtccatg atgcctgata
    13081 ttctctccga tccgaagagg cgcatcaggt cgtcctcgag cgagacgaag aactgtgatg
    13141 atccggggtc accctggcgg cctgcacggc ccctcagctg gcggtcaacg cgccgcgact
    13201 catgcctttc ggtgcctatg attgcaagtc cgcctgcttt tttaacctca ggtgtcagtt
    13261 tgatgtcggt accgcgacct gccatgttgg tagctatggt tactgtgccg gtctttcccg
    13321 cttctgcaac tatctctgcc tcacgctggt gcagcttggc attcagcacg ttgtgcttca
    13381 gccccttcat cttgagcatt cggctaagca actcggatat ttcaacagag gttgtaccga
    13441 caagaacagg ccttcccgcc ttgttcagtt ttactatctc gtcgatgaca gcgttgtatt
    13501 tttcgcgttt ggttttgtag acaaggtcct catggtcatt cctgatcact gccacgtttg
    13561 tggggatgac cactacgtct agcttgtaga tgttccagaa ctcacctgct tcggttatgg
    13621 ccgtgccggt catgccagcc agcttgtggt acatcctgaa gtagttctgc agggtgatgg
    13681 tggcgtaggt ctgggtggaa gcctccacct tcacgttctc ctttgcctcg atagcctgat
    13741 ggagtccgtc tgaataacgg cgcccctcta ggatgcgtcc cgtctgctcg tcgacaatct
    13801 tgaccttgtt atccatgacc acgtactcca catccttctc gaagagggtg taagccttca
    13861 tcagctgggt catggtatgg acgcgctccg acttgactgc aaagtcctgc aggagttcat
    13921 cctttgcctt cctcctctca ttctcgtccc tgactgactt ttcgatgtca gcaatcttgc
    13981 ttccgacgtc cggcagaacg aagaagctgg agtcctcgac ggatgttgag atcaggtcat
    14041 gtcccttttc agtcagctcg atggagtttg ccttttcgtc gatgacgaaa aacagctcct
    14101 ctgtaacttt aggcatttcc ttggagttgt tctgcatgta gaagttctct gtcttctgga
    14161 gcagtgatct taccccttcc tcactcaggt actttatgag tgcactgttc ttcgggaggc
    14221 ctttgtaagc tctcaggagg agcagtccgc ctttttcgtt gtcgccttcg gcgataagcc
    14281 tcttggagtc ggcaagcagc tgagtcacca gtttgcgctg ggcgcctacc agcttctcga
    14341 ccttgaactt gtactcgtcg aactgggagg tatcactttt ggcagtgggg ccggagatga
    14401 taagtggcgt acgggcatca tcaatgagga cggagtcgac ctcgtcaacg atggcgtaat
    14461 ggtgtttcct ctggacgaga tcctcggggt tgattgccat gttgtcacga aggtagtcga
    14521 agccgaattc gttgttggtc ccgaaagtga tgtccgagtt gtatgccttg cgtcgtgcat
    14581 gagagttagg ttcatgctta tcaatacagt cgactgacag tccgtggaac tcatacagcg
    14641 gccccatcca ttcggagtca cgtttcgaga ggtagtcgtt aaccgtgact atatggacgc
    14701 ctttccctgc cagggcgtta aggaacacgg gcagagtggc cacgagggtc ttgccctcgc
    14761 cggtagccat ctcggcaatc ttccctttat gcagcgccac cccgccgatg agctggacat
    14821 catagtggac catgtcccat actatctcat tgcctcctgc catccagcgg tttttccagt
    14881 atgccttctc tccctccacg gttattgagg ggcgtgtggc tgccaggttg cggtcccagt
    14941 cccgggcggt cacctcaagc acatcgttct ccttgaagcg gtgcgctgtt tccttgacta
    15001 ttgcaaatgc cacggggagg atttcgtcga gcttgttttc gatcttttcg agtacgtcct
    15061 ttgccagttt gtcaacctgg ttatagagtt cttccttccg gttgacatct tcctcattct
    15121 cagcactgac cctcagggcg gcaatttcct tctcctcggc cgagatatgg tcgcgtattt
    15181 cctgtttcag tgctgctgtc ctgtcacgga gtgcatcatt ggaaagcgaa gagagcagag
    15241 cgctctcctt ctgtatctta tcaacgaacg gattgatttc cttgaggtca cgttcctcct
    15301 tgtttccgag gaacaccgag aggactttgt taatgatgct catttactgt ctttttaaaa
    15361 acaatcgcaa aaataatata ttttatggtt ccatggggaa gaaaggttcg tcaggtgagg
    15421 cactgtcagc ccgcagggct tacataaatg caaaccgggg gaccgcaaaa ccataaaaaa
    15481 accggggcct gccccggttt ttattgtaac tgttgtcagc agccttctac ttacccctga
    15541 gattcatgat gtcgcgatca aggaagtaaa ctccgtgctg acctgcgaga ttaagcttat
    15601 cgataatctg ctgggcgctg ctctcctctt ctacctgttc cttgacgaac cactgtatcc
    15661 agttggcggt tgcgtgatcc ttgtcagcaa tggcggcctc caggacctcg tttatgctgg
    15721 cactaatgaa cttctcatgt ttaagcacct cgtcgaacat ttcggggata cccttgtatt
    15781 tcaccggggg cttcctgaat tcgggcacaa tggctgagcc gttgcgttca tttacatatc
    15841 tgaaaaactt gagcatatgt gctctctcct catcagcctg tgcatagagc catgctgcaa
    15901 tgcctgacat tcctttggtc tctgcccatg aagccatggc aagatacagg tttgatgagt
    15961 aaccttctct ttcgatctgc ctgttgcaga gctcttcaac ttttttcgtt aacatatttt
    16021 tatgtgttta atggttcttt tttcctcaga aacaagccag gaatgataat gtttattcca
    16081 gaagttcctt tactttcatc accagttctg aaggaaggaa tggtttggat ataactgcat
    16141 cggctcccag tttgctggcc atcatcaggt aacttccggg gccggccatc cctccgccgc
    16201 tgatggcaat catccgggtt gacggcttga tttcacgaat cttcataatc accatcagcc
    16261 cgtcctcctc cggcatcagg aggtcagtaa ttacaaggtc aacaaccgat gggcggaatt
    16321 ttgccagtgc ctctcttccg tcagcagcag caataactgt atacttacgt ttttcgaggg
    16381 catccctgag cagttcgcgc agggcggaat catcctcaac tatcattact cctgccatat
    16441 gatttcagtg acctggtccg gtggccgaag ttaatgtttt tcagggagag tgccaaaaaa
    16501 ggcgccgggt atgataatgt caaaagcagt tcctgcaccg ggttctgaac tgacggtgat
    16561 ggtgcctccc atctccctga tggcatcagc aactactgtc aggccgagac ctgttccatg
    16621 ctccttgccc gccgagacga acggtttgaa tatctcccgc tgcaatgctt catccatccc
    16681 ttttccggtg tcgctgaagc ggatatgcag ttccctgcac tcaccgtcat ggtgaatggg
    16741 cttaacgttt tcgcggcagc cgagggtgac tgtcagggtt cctccttttc ccgtcatcgc
    16801 ctggatggca ttcattgtca ggttcagaaa aatcctgaag agctgggcag gcacggcatt
    16861 cacatatgcg tcaggggctg tcacacggcg cattattcgt attccctccg gaaccgatgg
    16921 ccttataaaa tcaattgtgt cggcaagcac cgcagccacc ctgaccggca ccttttcacg
    16981 ggctgcatga cgactcaggt caagaagctg acctgtcagc aacctggccc tgtcggctgc
    17041 gccgatgatt ttccgtatgt tcccggcagc ttcaggggag ccggtaaggc tctcgagcgc
    17101 catctcactg tatccgtaaa tagtggtaat tacggtattg aggtcatgtg cgataccacc
    17161 ggcaagcgct cccagccgcg agccggctcc cgggtcactc ccgggggttt tctgactgtt
    17221 gtcagcctga tccttatctt tcctggtgct caccctcttc cgttgagtgc ctattcaaaa
    17281 tcgaggttga atgttttctt caggtccctg agagccggat tctttgctgc caggtgattg
    17341 aacttctgtt cgggagagta gagaatttct cccctctcgg tggcttccac ggtggtctga
    17401 agcctgagag agccgttccg gagtgtccgc cgcaggtgac cttcaagtcg cggcttgaag
    17461 ttatgatcaa aggtttcctt gagggtcagg ttgtcaagct tgagcagtac agtcttgtcg
    17521 gcaagcagtt caggagtcac ggaagagagt gtcacagaga tccttgggct ctcctccttt
    17581 atttcttcgg cgaagctggc ccatgcctcg cgaagctcct ccgcggtgaa ttcgagatca
    17641 tgggagatca atgtggtatc aggctgtggt tcactgacaa ttgcctccgg tgacttagcg
    17701 cctgcaatga tgttttttat cgaaaccttt ttgggctcat cgccggtggc agggggtttt
    17761 ggtctctgta tggccggtcc cggcgaaggg gatgcttcag gtggtgtccg gactggcgtt
    17821 gtctggggtg aagcaccgga gcgcggcgga gccacactga aatcctcttc ctcctctaaa
    17881 cggcccggtt cagctttttt ttttacctct tcctgtgctc ctgctgccga aaggttacac
    17941 atgcggagga gcgccagttc gacgtgcagg cgctggttct tgctggcccg gaagttgagc
    18001 atgcactgcg atccgatgtc gagagcttcg tagatgaact gcagcggtgc cttcacagcc
    18061 tgttcgcggt agcgctgcct tattgaaggg gatgtctcaa gcagcttcac ggtggcctcg
    18121 tcgcggctta ccagcaggtc gcggaggtgg ttattgagtc ctgccatgaa gttatacccg
    18181 tcgaaaccct tgttgaggat ctcgctgtaa gtaaggagca cagatgtcac atcgccgtcc
    18241 atcgcggcag cggtgattcg gaaatagtac tcgtaatcaa ggacattgag gttggcgatc
    18301 acatcggcat aggtgattgt cttgccgctg aagctcacta tctggtcaaa gattgagagc
    18361 gcatcgcgca ttgcgccgtc agcctttgtg gcgatgacgt ggagggcttc atgttcggcg
    18421 ttgaccccct cgttgcctgc aacccagctc agccgctgca ctatgtcatc aacgttgata
    18481 cggttaaagt cgaaaatctg gcagcgcgag agtattgtgg ggatgatctt gtgcttttcg
    18541 gtggttgcga ggatgaagat ggcatgtgat gggggctctt cgagggtctt caggaaagcg
    18601 ttgaaggcct gggtggagag catgtgcacc tcgtcaatta tgtagacgct gaaccttccg
    18661 atctgcgggg gtatgcgcac ctggtcagtc aggttgcgga tgtcgtcgac actgttgttc
    18721 gaggcagcat cgagctcatg tatattgaag gaagcctgtg aattgaatga cttgcatgat
    18781 tcgcagttgt cgcaggcttc gccgtcaggc ccggtatccg agcagtttat ggtctttgcg
    18841 aagatcctgg cacaggtggt ttttccgact ccgcgggggc cgcaaaaaag gtaagcctgg
    18901 gccagaaggt tattcctgat tgcattccgc aaagtggtgg tgatggtatc ctgcccgaca
    18961 accatgtcaa aggtggcagg cctgtatttg cgggcagaaa caataaagtt atccataccg
    19021 ggttttaaca gtacatgtac atatccgtca caaatatagt caaatagttt tcggaatatt
    19081 tttttttaag atttattgtc ttacttttgc aacccgattt attaacacta atataaacca
    19141 attaagagat gttgaatcag tacgaaaccg ttttcattgc aactcccgtt ttgtctgaga
    19201 atcagatgaa ggaagcggtt cagaagttca agaagatcat cactgacaat gaaggtgaga
    19261 tcattcatga agagaactgg ggtctgaaga aactcgctta tccaatccag aagaaatcga
    19321 c
//
LOCUS       DAOPXU010000048        19298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_2966_length_19298_cov_12.840358, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000048 DAOPXU010000000
VERSION     DAOPXU010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19298
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2966_length_19298_cov_12.840358"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PKK03_06505"
     CDS             complement(<1..628)
                     /locus_tag="PKK03_06505"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNX84088.1"
                     /translation="MTKDSSAGEKKRLLLSMLIPFMLVFLMWLMTGASLLFGTEFHFL
                     GIYPGRVSSLTGIITSPFVHSGFRHLFNNSLPLFILGTALFYFYSQVSFRVLLWVTLL
                     TGLAVWITGRPAWHIGASGIVYGLAAFLFVSGIIRRHIPLMGLSLLVAFIYGEMVWGI
                     FPGFRMDISWESHMLGAAAGVVLAIWYRHEGPQKPVPFYERDEENEAGE"
     gene            complement(650..1633)
                     /locus_tag="PKK03_06510"
     CDS             complement(650..1633)
                     /locus_tag="PKK03_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX84089.1"
                     /translation="MKAYNWGILGTGNIAHKFARALMLLDNAHLYAVGSRDAGRAEKF
                     AGEFGFSRHFGTYEEMLADPGVEVVYISSPHSMHMEHTLMALRNGKHVICEKPMSITA
                     AEVSLMSDEARRSNLFLMEALWPPFQPSYLKAEEILRSGKMGRLLHMRGKFAFVSPYD
                     PELRTYNLALGGGSLLDIGIYPVMDILRYLGEPENIVATSVFAPTGADESTAMIFRFS
                     DGRMAEAYSSFGNMGGVSTEFYCEKGNLILTRGRDRNQHLIIEMQGDVDDRIFTPPAQ
                     GYQYEAAEVMNCLDHGLAESPVVPLSFSLSLARILDEVRRKTGIRYPGRDR"
     gene            1830..2405
                     /locus_tag="PKK03_06515"
     CDS             1830..2405
                     /locus_tag="PKK03_06515"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNX84090.1"
                     /translation="MTVNINLGGYAFNLDDDAYERLRQYMKRLEKEFSGEPGATEIMS
                     DIEGRISELFRGKLSSYKQVITMKDVEEVMAILGSPEDISGGEPAGEVPPRSQRRMYR
                     DPDRRIIGGVCAGISAYLDWDPLIIRIIFAALIFAGGFGLLLYLILWIVLPEAKTTAQ
                     KLEMRGDPVTIENIKDSVRQEFNTVKEKMNL"
     gene            2448..2777
                     /locus_tag="PKK03_06520"
     CDS             2448..2777
                     /locus_tag="PKK03_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNX84091.1"
                     /translation="MNTDNTRAQMRKGILEYCILAVLSRNSCYAVDIINELKKAQVIV
                     VEGTLYPLLTRQKNAGLLSYRWEESPQGPPRKYYELTELGRIYLRELDKVWDELVESV
                     NQIRDRK"
     gene            2786..4057
                     /locus_tag="PKK03_06525"
     CDS             2786..4057
                     /locus_tag="PKK03_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84092.1"
                     /translation="MKKNLDINIAGQLFRVDEDAWEVLKHYLDHVSARFKTEQGGDET
                     LADIEARIAEIFGGGKEPPTLVSKEMVTDMINIMGAPEDYYDDGPAAKNKTLYVRKSM
                     YDPNSFSARVGRGLSEFFRAFGKLISAILRVFAIILGALFTLFGFLLFFTFVIVIFFN
                     NVPFFSSVMEPQMTNVHDMLGIVLSTNAVWPILILTALVTLLPLAALIWLGVKLIFRI
                     KERFRVMNIILFLVWIASLCALAIILILRLSVYSESESVEKRLTLDPAPETLWINTMK
                     KQTGLSYYRYASVEDFRFFMESPDGVLRVSPDLSIRGSDNGTGYISVERRAYSNSDTE
                     AVRNARQIEYNWKMSGDTLYLDEYCTLPAGARWNGSMVDIDIRLPEGTEIRFVQGVSP
                     DVLNLHLFSGENPAWKIVDGYPRSIDDWTDQ"
     gene            4172..5668
                     /locus_tag="PKK03_06530"
     CDS             4172..5668
                     /locus_tag="PKK03_06530"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HNX84093.1"
                     /translation="MTVTPENILLIASVLIFISVLAGKTSFRIGVPTLILFLAVGMLA
                     GSEGIGKIPFDDPGLAQFIGIVALNFILFSGGFETDWNSVKPIMWQGIALSVMGVLLT
                     ALSIGLFVWWVTDFTIYEGLLLGAIVSSTDAAAVFSILRSKSLALKGHIRPTLEFESG
                     SNDPMAYILTIVFIGLVQNGEQSFWPVLLMFFTQLLLGGAFGYLFGLGSKYVINHIKL
                     DYEGLYPVLSITLMILTYSVTTAIGGNGFLAVYISALYLGHQDLMHKRTIMKMFDGLA
                     WLMQIILFVVLGLLVFPSHIVPVIGIGLLVSAFLIFIARPVSTFLILAFFRVRTRTKL
                     FISWVGLRGAVPIVFATYPLMAGLEKAEMIFNIVFFISLTSVLLQGTTVPLVAKWLHV
                     SLPARVKTVTAADVLLSEPMNSEFAEFTIAPESVVAGMKIIDLGFPQNARIAMIKREN
                     KYLIPDGMTVIQPGDRLIVLAGSKNILEAVTECLLSDNPVCSDTAKPS"
     gene            5720..7705
                     /locus_tag="PKK03_06535"
     CDS             5720..7705
                     /locus_tag="PKK03_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNX84094.1"
                     /translation="MELGVLKDIVIIFALSTFVNFIFTRIRIPAIIGYLITGVIAGPH
                     LLRLISSPENVEVMAEIGVILLMFTIGLEFSLNHLLNIRKVVFLGGFMQLLLTAGVTM
                     FTARVFHFEWTESIFVGFLTALSSTAVVLKLLQERSELTTHYGKTVVGILIFQDIILI
                     PLLLFTPMLGGATADVGHDLLILMLKTAGILIFIWAGTKWLMPKVLHLIAMTRSHELF
                     LMSLLLICLAVAMLTHELGMSLAFGAFLAGLMISDTEYSHSAFGNLIPFKDVFTSFFF
                     VSIGMLLDLGFVADHPGLVIGSVLLVIILKAFIAGMTAFTLGHTFFGTVVVGLSLAQV
                     GEFSFILAGLGLTHNIITPVHYQLFLAVAITSMALSPLLIQTSKPLANLILKLPLPPY
                     IVNGLFPLKQVEIPEISKHVVLIGKDSRAINLAEMIRSMELPFTSVVFDTDRARAEME
                     KGNLTVFGDATFEPVLREAFVHTAELVIVSVGNLITALSIVEHIRSMNKHAHVIVRTR
                     HVTDVEELYRLGASQVIPEEFETAIDFFERILGKYLIPRMDIERAIARVREDNYGIFR
                     DRGKVRGYSLLKDIPDIEIAAVRVGGESLFAGKTIAETAMRKTAGVTIVAINHAGNIT
                     ANPEPSAFIHGNDIVYLLGQPEMIALASNILSEPRPH"
     gene            7911..9536
                     /gene="pruA"
                     /locus_tag="PKK03_06540"
     CDS             7911..9536
                     /gene="pruA"
                     /locus_tag="PKK03_06540"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HNX84095.1"
                     /translation="MNNAIYSIHRPENEPMMGYARGSRERDELEKELKRISSETIEIP
                     LLINGKPVKTGRTGKVVMPHDHRHVLATYHMAGEKETADAIDAAMKAKEAWLTLSWIE
                     RASIMLKAAELLSKKYRYTIVAATMLGQSKTVHQAEVEAACETIDFLRFNSWFAGQIY
                     QEQPRSASDQLNRVEYRPLEGFVYTITPFNFTAIASNLNMAVALMGNTTVWKPATTSL
                     FSSWILMKVFMEAGLPEGVVNFLPGQGSVISSVVMKHPDLGGIHFTGSNSTFNALWKT
                     AATNLDKYKSYPRIVGETGGKDYIMIHNSANPQEVATAIIRGSFEYQGQKCSASSRVY
                     IPRSLWPEISVHVKRQAEEIKMGDPADFRNFMNAVIDEKSFDNIMHYIDEAVKSPECE
                     IFHGGKGDKSKGYFVEPTIILTKDPHFITMKEEIFGPVVTLYIYEDDRYEETLRLVNE
                     TSPYGLTGSIFSTDRPAMVKACQVLRYAAGNFYINDKPTGAMVGLQPFGGARASGTND
                     KAGGRLNLIRWVSPRTIKETFLPATDFRYPYMD"
     gene            9764..10942
                     /locus_tag="PKK03_06545"
     CDS             9764..10942
                     /locus_tag="PKK03_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNX84096.1"
                     /translation="MKKILLCAAAVALLLGACERTPEPFIQEDDYINTSLEVIPGQYV
                     VLLKGEFGYVKSGTMSYDDAQLAMTALTKDILAKAGIYDREPLQVYSKAIQGFAVNLS
                     DSEVAALEKNPNVKGIWPDQMFTLAKPGTTPTPPAAQATPPGITRVGGPVNYTGSAKA
                     WIIDTGIDLDHPDLNVDGGSGRNFISTLKTPDDDNGHGSHCAGIVAAKNNTIGVVGVA
                     AGATVVPVKVLDRRGSGAYSVIIAGVNYVAGAATPGDVANMSLGGGAYEPIDLAVINM
                     AAAGIRVALAAGNESDNANNHSPARANGVNIYTTSACDSKDVWAYFSNYGNPPIDYCA
                     PGVNIYSTYKGGGYTTMSGTSMAAPHACGVLLVTNGNPKTSGYVKNDPDGDADPIIHN
                     "
     gene            complement(11052..13283)
                     /locus_tag="PKK03_06550"
     CDS             complement(11052..13283)
                     /locus_tag="PKK03_06550"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNX84097.1"
                     /translation="MKKALACILLLLTVVTGSRSQPSLPDNGQLFTDTVVPVVYITIN
                     PDTLAWLYANPYSDREFRSVFVFDNGTVRDTIDPAGFRLRGNTSRDSKKKSFKVSFNT
                     FTDGGKYHGVEKMNLNGEHNDPSVMRSKIMWDILRKWDVPAPRANHVRVYINGNYYGL
                     YINVEHIDEEFALSRFGNKDGNLYKCLYPADLNYRGDDPDSYRIWNGSRWIYELNTNE
                     EENDFSDLAGFIGILHNTGNEALLCELDRVFNVQDYLKIMAVQILCGDWDGYIYNKNN
                     FYLYHNTLTGRLEYIPYDVDNTFGIDWFGIDWATRNMYGWQPGGDQQRPLYDRIINNP
                     VLREQFSFYAARLVGGVLDLDSLIQSVDARKEMISPYIANDPYYPRDYGFDHSDFLNS
                     FTSAFGAHVKWGIYPYLQVRLASMASQAEEADPDPVIKYIAHDRKAGEPLYISCTVEA
                     ISASAAVKLIYTVAGGTSLEAEMSTSGSGRYSVTLTGIPDEDEVAYRVQATGGDGEMS
                     LLPCVAALAAPVGGETPLLFINEFMADNEMTVADEHGYYSDWIEIYNGGNEAVDLGEI
                     CLTDNFNDPLKWRMPSVVLPAGGFTIFWADGIPGLGEKHAPFKLDKEGEEIGIYTLNK
                     LMVDTVSFGLQQEDISTGRKSDGAEEIVFLATATPGRSNNITSAGSTYADMGVKIYPN
                     PVIGETLWLSEAADCRIYSITGVMLFSGTDMTEIDVSVFPAGIYILVTGDGRAVRFVR
                     AGR"
     gene            13339..13878
                     /locus_tag="PKK03_06555"
     CDS             13339..13878
                     /locus_tag="PKK03_06555"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNX84098.1"
                     /translation="MSSKPLRFTDGVVTIRKFRRSDKFRMAEIANNEKVAANLRDAFP
                     SPYTLEDAQKFISMCLRQVPYGIFAIEFEGEYVGNIGLHRQGDVYRKTAELGYFIGEP
                     YWHKGITPRAVNLICEYGFRELDVIKIFSGVFSFNTASQRVLEKCGFTLEAVLKAAVI
                     KNGKICDEYRYARILDGYK"
     gene            complement(13910..14296)
                     /locus_tag="PKK03_06560"
     CDS             complement(13910..14296)
                     /locus_tag="PKK03_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HNX84099.1"
                     /translation="MAYYYSKTLRTSFDDAVEKVTASLSAEGFGVISEVDLHEKIKYK
                     LGVDFKRYRILGACNPAFSYRALQAEDKIGVMLPCNVLVIEQGEGETEIAAVNPSEAM
                     SVVRNEAVNQVAAEVGEKLRKALDSL"
     gene            14464..16191
                     /locus_tag="PKK03_06565"
     CDS             14464..16191
                     /locus_tag="PKK03_06565"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNX84100.1"
                     /translation="MKTIPESLLTWLFCLICIACSERRDSGNFIETFNADFRAELAGL
                     HVSNASYAVFCTDSVILQDSFSAAGTGTDGDSPFLIGSVTKVFTAVAIMQLYQEGKID
                     IDRPVSDYVPDFQIRQRFPESAPITLRAVMTHHAGIPSDNYLHKFSKMPNDFIDLPGY
                     LNTLYTCYPVGEIWAYSNLGYALLGVVIERVSGMRYGEYIARHIFEPLGMNSSGLYSD
                     YGSQKSLSAAYDGKGNLTTELPICDKPAGAIYSTVTDMIRFGRAFIDGKEILLRKEFL
                     DQMFELQNADNLLDLDHRSALCFNFKNKAPELGRLLEHGGATMYHRAQLTIAPDAGLA
                     CVLLSDSPNGKDNAWRLDEQLMVEYCRSAGITPDRRFNREKKMLFTPVSSKNLQSFAG
                     NYAMPGLVCRIDWKHNLLSPTIDGQNFYLAQHDSGSFIPAKWFLGMLFKSKRMYFLLE
                     EINGEKHFIQVMPWGGLNIIGTRVTPEPVSETWKNRAGDYVVSNAAPGDAPTIEKMSI
                     SVEDSFVVLRYGFSPEMSFGQNASVALNLVNDNEAFVLGYGRGGGESVIFGPDGRSFS
                     YMGLKFVKI"
     gene            16188..17036
                     /locus_tag="PKK03_06570"
     CDS             16188..17036
                     /locus_tag="PKK03_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNX84101.1"
                     /translation="MKKSTFFAILVAFALCAGAIKGQKQVKHQNDWQLVWQDEFNGTR
                     PDTSKWNVLIRETSKHNELQYYVPDEVYVENGILRIRSRVRRYGSKEYTSGRLDTEGK
                     FAPVYGRFEIRAKLPGGQGLWPAHWLYPQERDWLMEKTMEDAVAAGKERLIPEERPWY
                     TEIDIMEFLGHEPSVLYGTIHYCAFDGTRRSSSGIWRGTVDYTKDFHTYVLEWEPDSI
                     RWFIDGNRIHATASGIPHKPHYLILNTAVGGGWPGNPDSTTVFPQYHDIDYVRVYAKE
                     SYFPQQ"
     gene            17100..17672
                     /locus_tag="PKK03_06575"
     CDS             17100..17672
                     /locus_tag="PKK03_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HNX84102.1"
                     /translation="MSETGKHKEPAPQELQQLLTLLRTRFMKNPARHKGIEWETVQQK
                     LESNRLKLRSLFEMERTGGEPDVVASDSTTGQIVFNDCSPETPKERRNLCYDREALDS
                     RKEFKPSGSATDMAAAMGIELLTEEEYLHLQTLGDFDTKTSSWIKTPADIRKLGGAIF
                     GDRRFGRVFIYHNGASSYYAVRGFRGSLRV"
     gene            17772..18377
                     /locus_tag="PKK03_06580"
     CDS             17772..18377
                     /locus_tag="PKK03_06580"
                     /inference="COORDINATES: protein motif:HMM:NF019145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84103.1"
                     /translation="MKRIVIPLLLVLTVSCEMLSDCPKLVKGKPEGHLVDDSELKTIK
                     TLFRASNLSYENYQFYSLECILSGEFYVSCHQYVNDLKVWTDDLFFHFDKNGRFESLS
                     GDIVSAIDAGTSPSMDVTQAESRFLGRMNEDSFFRNSKSVANGCITCELVYYDIAGMS
                     GGSTPDYVLAWLVKPEKSDYPVAVVKDSDQSVLYYNNGIIF"
     gene            18420..19211
                     /locus_tag="PKK03_06585"
     CDS             18420..19211
                     /locus_tag="PKK03_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNX84104.1"
                     /translation="MTRNYYFQDIVRSRTATFDIFGVGLKKHHISAMLEFDVTESRSG
                     LRELRKKGISISFNAWLIKVIGSVIAKHPEAAAYFYNKRKIIIFNDINVSLIVEKKIG
                     DARVPIPLVIEKVNKKPAAEISEEIDAAKGRELSSGDIVLKKKSSAYERFYYRMPGFF
                     RRFFWVVLLKRPKAAFRTMGNVSVTSVGMIGKVNGWFIHKSVHPISFGVGSVLMKPVV
                     IGNEIKAREVLNMTVLVDHDVIDGAPMVRFLGDLTDFIENGKEMD"
BASE COUNT         4892 a         4939 c         4942 g         4525 t
ORIGIN      
        1 cttctccggc ctcattttcc tcatcgcgtt catagaatgg caccggcttc tgcggccctt
       61 catgacggta ccatattgca aggaccaccc cggcagccgc accgagcata tgcgactccc
      121 atgatatgtc catcctgaat cccggaaaaa ttccccagac catctcacca tatataaagg
      181 caaccagcag tgacagcccc atcagcggga tgtgcctgcg gataatacca ctgacgaaaa
      241 gaaaagcagc cagaccgtaa acaatgccgc ttgccccgat atgccatgcc ggccggcctg
      301 taatccagac agccaggcct gtgagaagcg tgacccacag cagtaccctg aatgaaacct
      361 gtgagtaaaa gtagaacagc gccgtgccaa ggatgaaaag aggaagggag ttgttgaaca
      421 ggtgcctgaa cccggaatga acaaacggag aggtgatgat gccggtgaga gaagagaccc
      481 tgccgggata aatgccaaga aaatgaaact cggtgccgaa cagcagcgac gcccccgtca
      541 tcagccacat gaggaagacc agcatgaaag ggatgagcat gctgagcagc aggcgcttct
      601 tttcaccggc agatgagtct ttcgtcatgc cgttcagaag ttgattacac taacggtcac
      661 gtcccggata ccttatgccg gtcttccggc ggacctcgtc aaggatgcgg gccagcgaga
      721 ggctgaaaga gagggggacg acggggcttt ccgcaagtcc gtggtcaagg cagttcatca
      781 cctcggcagc ctcatactgg taaccctggg cgggcggggt gaatatgcgg tcatcaacat
      841 caccctgcat ctctatgata aggtgttgat tacggtcacg gccacgtgtg agtatgaggt
      901 tgcccttctc acagtaaaac tcggtggaga caccgcccat gtttccaaat gagctgtagg
      961 cctcggccat gcggccgtca ctgaacctga agatcattgc cgtgctctcg tcagcgccgg
     1021 ttggggcaaa tacagaagtg gccactatgt tctccggttc acccagatac ctcaaaatat
     1081 ccatcaccgg atatatgccg atgtcaagca gtgaaccacc accaagggcc aggttgtagg
     1141 tgcgcagttc cggatcatac ggagaaacaa aggcaaactt accccgcata tgcagcagcc
     1201 gtcccatctt cccgctgcgg aggatctcct ctgccttaag gtacgatggc tggaacgggg
     1261 gccataatgc ctccataaga aaaagattgc tcctccgcgc ttcatcagac atcagtgata
     1321 cctctgctgc cgtgatcgac atgggctttt cacagatgac atgcttgccg ttgcggagag
     1381 ccatgagcgt atgctccatg tgcatggaat gcggcgagga gatgtaaacc acctcaacac
     1441 cggggtctgc cagcatctcc tcataggtcc cgaaatgcct gctgaacccg aactccccgg
     1501 caaacttctc tgccctgccg gcatcacgcg acccaaccgc ataaagatgc gcgttatcaa
     1561 gcagcatcag cgccctcgca aacttgtggg caatgttgcc tgtccccagg attccccagt
     1621 tgtacgcttt catatccttg cttttttcta taaagttagt cattcatccc ctatctaacc
     1681 atagcccggt tgatgagttc aaaatccact caaaaaaaat attttaaaaa atttacttct
     1741 atgtattgca tagtattatt tttattttat atatttgcaa taccaaatta acttgcatta
     1801 acaggtacct tataataaaa acgatgaaaa tgactgtcaa catcaatctc gggggatacg
     1861 cattcaacct cgacgatgac gcttacgagc ggctccggca gtacatgaag cgcctggaaa
     1921 aggaattttc aggcgagccg ggggctactg agataatgtc cgatatcgag ggaaggatat
     1981 cggagctgtt cagggggaaa ctcagcagct acaagcaggt cattacaatg aaggacgtgg
     2041 aggaggtgat ggccatactg ggatctcccg aagacataag cgggggtgag cctgccggcg
     2101 aagtgccgcc caggtcacag cgcaggatgt accgcgatcc tgacagaagg ataatcggag
     2161 gggtgtgtgc cggcatctcg gcatacctcg actgggatcc gctgataatc aggatcatct
     2221 tcgcggcgct gatattcgca ggtggcttcg gccttctgct ctaccttatt ctgtggatag
     2281 tgcttcccga ggcaaagaca accgcacaga agctggagat gcggggtgat ccggtaacca
     2341 ttgaaaacat taaggattcg gtcagacagg aattcaacac cgtaaaggaa aagatgaatc
     2401 tctgaccttc gccgaaggcg taaaaagctg acaataaaga acaagccatg aatacggaca
     2461 acacaagagc acaaatgcgc aaggggattc tcgagtactg catactggca gtgctgtcga
     2521 gaaattcctg ctacgccgtt gacatcatca atgagctcaa gaaagcccag gtgatagtcg
     2581 ttgaaggaac actctacccc ctgctgacca ggcagaagaa cgcagggctg ctcagctacc
     2641 gctgggagga gtcgccgcag ggccctccac ggaaatatta cgagctcacc gagctgggac
     2701 ggatatatct cagggaactc gacaaggtat gggacgagct tgtggaatcg gttaaccaga
     2761 tacgtgacag gaaataaagg cagccatgaa aaaaaacctt gacatcaaca ttgccgggca
     2821 gctcttccgt gtggacgagg atgcctggga ggtcctgaaa cactatcttg accacgtctc
     2881 tgccaggttc aaaaccgagc agggaggaga tgagacactc gcagacatag aagcgcgcat
     2941 tgcggagatt ttcgggggcg gcaaggagcc tcccactctt gtctcgaagg agatggtaac
     3001 cgatatgatc aacatcatgg gtgcaccgga ggattattat gatgacggac cggcagcgaa
     3061 gaacaagacg ctgtacgtaa ggaaatcaat gtatgatccc aacagcttct cggccagggt
     3121 gggcaggggg ctgtcagaat tcttcagggc attcggaaaa ctgatttcgg caatcctgag
     3181 ggtatttgca ataatactgg gagcgttatt caccctcttc ggattcctgc tcttcttcac
     3241 cttcgtgatt gtgatcttct tcaacaatgt cccgttcttt tcctccgtga tggaaccgca
     3301 gatgacaaat gttcacgaca tgcttggcat agtgctcagc accaatgccg tatggccgat
     3361 actcattctg acggcgctgg tgacgctcct gccgctggca gctctcatct ggctgggcgt
     3421 caagctgata ttcagaatta aggagagatt ccgtgtaatg aacattatcc tcttcctggt
     3481 ctggatcgct tcgctctgcg ccctggcaat catactgatc ctccgcctct cggtctattc
     3541 cgaaagcgag tcggttgaga agaggctgac actggatccg gcgcctgaga ctctctggat
     3601 aaacacgatg aagaagcaga cgggcctgag ttattacagg tatgcttcgg tggaagattt
     3661 caggttcttc atggagagcc ctgacggtgt gctgcgcgta tcgccggatc tgagcatccg
     3721 cggcagcgat aacggcaccg ggtacatctc ggtggagaga agggcttaca gcaactccga
     3781 tactgaggcc gtcagaaatg cacggcagat cgaatacaac tggaagatgt ccggtgatac
     3841 cctctacctc gacgagtatt gcactcttcc ggccggtgcc aggtggaacg gatccatggt
     3901 tgatattgac atacggctcc ctgaagggac cgagatcagg ttcgtacagg gggtttcacc
     3961 cgatgtcctt aacttacatc tgttttcagg ggagaacccg gcatggaaaa tcgtggatgg
     4021 ctatccgcgc tcgatagacg actggacaga tcaataagtc tgcacctgcg tattttttac
     4081 tcttcaatct cccggcctga acaaccggga ttttttttgt ggcaaaaccg cttttttcta
     4141 tctttagctg cttgaaaaaa cataacagcc ggtgactgtc actccagaaa acatactgct
     4201 gattgcttca gttctgattt ttatcagcgt gctggccggc aagacctcat tccggattgg
     4261 agtgcccacc ctgatactct tcctggccgt ggggatgctg gcaggatcgg aagggatagg
     4321 gaaaatccca ttcgatgacc ccggccttgc acagttcata ggcattgtgg cgcttaactt
     4381 catcctgttc tccggggggt tcgagaccga ctggaactcg gtaaaaccca ttatgtggca
     4441 gggaatagcc ctctccgtga tgggtgtttt gctgaccgcc ctgtctatcg gactctttgt
     4501 ctggtgggtg actgatttca ccatctatga aggactactg ctcggggcaa ttgtttcatc
     4561 aaccgatgcc gccgcggttt tctccatcct cagatcaaaa agcctggcgc tgaagggtca
     4621 catcagaccc accctggaat tcgagagcgg aagcaatgac ccgatggcgt acatactcac
     4681 catcgtattc atcgggctgg tgcagaacgg agaacagtca ttctggcccg tgcttctgat
     4741 gtttttcacc cagttgctcc ttggaggtgc tttcgggtat ctcttcggcc tcggaagcaa
     4801 atatgtaatc aaccatatca agctcgacta cgagggactc tacccggtac tgtcaatcac
     4861 cctcatgata ctcacttact cggtaaccac ggctatcgga ggaaacggat ttcttgcagt
     4921 ctatatatca gccctttatc tcggacacca ggatctgatg cataagcgta caatcatgaa
     4981 gatgtttgac ggtcttgcat ggctgatgca gatcattctc ttcgtcgtgc tggggctgct
     5041 cgttttcccg agtcacatcg taccggtcat tggcatcggg ttgctggtat cggccttcct
     5101 gatatttatc gcacggccgg taagcacctt tctcatcctg gcatttttca gggtacggac
     5161 caggacaaag ctcttcatct cctgggtggg gcttcgcggt gcagtaccga ttgtttttgc
     5221 cacctacccg ctgatggcag gtcttgaaaa ggctgagatg attttcaata ttgtcttctt
     5281 tatctcactt acttcggtgt tgctgcaggg cacaaccgta ccgcttgtgg ctaaatggct
     5341 tcatgtttca ctcccggcaa gagtcaagac cgttactgcc gcggatgtgc ttctctctga
     5401 accaatgaac tcggaattcg ccgaattcac aatagcgcct gaaagtgtcg tagccggcat
     5461 gaagataatt gacctgggat tcccgcagaa tgcccgcatt gcgatgataa aaagagaaaa
     5521 caaatacctc attcctgacg gtatgacggt cattcagccg ggcgacaggc ttattgtgct
     5581 ggcaggaagc aagaatattc ttgaggcggt gacggaatgc ctcctttcag acaatccggt
     5641 ctgctctgat accgcaaaac cctcctgatt catccaaccg catcttttca tatctttatt
     5701 gcatcaactg cagtaatcaa tggaactggg tgtcctaaag gatatcgtca ttatctttgc
     5761 actctccacc tttgtgaatt tcatcttcac aaggatccgc atccccgcca taataggtta
     5821 cctgataacc ggcgtcatag ccggacccca tctgctgagg cttatcagtt cacctgaaaa
     5881 tgttgaggta atggcagaga ttggcgttat cctcctgatg ttcaccatcg gcctggaatt
     5941 ctcactgaac cacctgctga acatcaggaa ggtggtcttc ctgggcggat tcatgcagct
     6001 gctgctcacc gcgggtgtga ctatgttcac ggcaagggtt ttccactttg aatggacaga
     6061 atcaatcttt gtaggtttcc tgacagctct cagcagcacc gccgtggttc tcaaactgct
     6121 gcaggaaaga tcagaactca ctacccacta cggcaaaacg gtggtgggca tactgatatt
     6181 ccaggatata atcctgatac ccctccttct cttcaccccc atgcttggcg gggcaacggc
     6241 tgacgtgggc catgacctcc ttatcctgat gctgaaaacc gcagggatat tgatcttcat
     6301 ttgggccgga acgaagtggc tcatgccgaa ggtgctgcat cttatagcca tgacacgaag
     6361 ccacgaactt ttcctgatga gcctgcttct catctgcctt gctgtagcta tgctgacgca
     6421 cgaactgggg atgtcactgg ccttcggggc attcctcgcc ggactgatga tctccgacac
     6481 cgaatacagc cacagcgcct tcgggaacct catcccgttc aaggacgtat tcaccagctt
     6541 tttctttgtg tcaataggga tgctgctcga cctgggattc gttgctgacc atcccggcct
     6601 ggtgatcggg tcagtgctcc tggtcattat cctcaaggct tttatagcgg gcatgacggc
     6661 cttcaccctg gggcatacct tcttcgggac ggtggttgtg gggctctccc tggcacaggt
     6721 gggtgaattt tcattcatcc ttgcggggct cgggctcaca cacaacatca tcactcccgt
     6781 tcattaccag ctcttcctgg cagtcgcaat cacttcaatg gcactgtcac cgctcctgat
     6841 acaaacatca aaaccgctgg ccaacctcat actgaaactg cccctcccgc catacatcgt
     6901 caacggcctc ttccccctca aacaggtgga aatccccgag atcagcaagc atgttgtgct
     6961 gataggcaaa gattcaaggg ccataaacct ggctgagatg atcagatcga tggagctccc
     7021 cttcacctct gttgtttttg acactgacag ggcacgggcg gagatggaaa aaggaaacct
     7081 gacagtgttc ggtgatgcca ccttcgagcc ggtgctgaga gaggcattcg tccacacagc
     7141 cgagctggtg attgtctccg tgggcaacct gataacggcc ctctccatcg ttgaacatat
     7201 ccggtcaatg aacaagcatg cccatgtgat cgtgcgtacc aggcatgtca cggacgtaga
     7261 ggaactgtac aggctgggag ccagccaggt cattccggaa gaatttgaaa cagccatcga
     7321 cttctttgag agaatcctgg ggaaatacct gattccgcgt atggacatag agcgggccat
     7381 cgcccgtgtc agggaagaca actacggcat tttcagggat cgcggcaagg tacgcggata
     7441 ctccctgctg aaggatatcc ctgacatcga gattgcagcc gtaagggttg gcggggaatc
     7501 cctgttcgcg gggaaaacca ttgctgaaac agccatgcgc aagacagccg gagtcactat
     7561 cgttgcaatc aaccacgcgg gaaatattac tgctaatcct gaaccatcag cattcattca
     7621 cggtaatgat atagtatacc tgctgggcca gcctgaaatg attgccctgg ccagcaatat
     7681 cctctccgaa ccccggcctc actgagagcc ctctccgaac cccggcctca ctgagaaccc
     7741 taaggcctga tcggccggac aggaccggcg aaggctgtat caatgagtga aaggcagccg
     7801 ggaacaattt ctgcatgaca gttgttgcta attaatagag acttaattat tataattgct
     7861 cctgattttc gatatgcata aatctgcata ttgaaataaa ccgaataaat atgaataatg
     7921 ctatctattc gatccacagg cctgagaatg agccgatgat gggttatgca cgcggaagcc
     7981 gcgagcgcga tgaacttgaa aaggaactga aacggatttc atcggagacg atagaaatac
     8041 ccctgctgat taacggcaag ccggttaaaa ccgggcgtac gggaaaggtg gtcatgcccc
     8101 atgaccacag gcatgtgctt gccacctacc acatggccgg agaaaaggaa acggctgacg
     8161 ccattgatgc tgccatgaag gcaaaggagg cgtggcttac cctctcatgg attgaaaggg
     8221 cctcgataat gcttaaggct gccgagctgc tttcgaagaa atacaggtat acgatcgttg
     8281 ccgccaccat gctcgggcag agcaagacgg tgcatcaggc cgaggttgag gcagcatgcg
     8341 agacaataga tttcctgagg ttcaacagct ggtttgccgg ccagatctac caggagcagc
     8401 cacggtcggc atctgaccag ctgaacaggg tcgagtacag gccgcttgaa ggattcgtct
     8461 atacaatcac acccttcaac ttcactgcca ttgcatcaaa cctgaacatg gccgtggcgc
     8521 tgatggggaa taccacggtc tggaagccgg cgacgacctc gctcttctcg agctggatac
     8581 tgatgaaggt gttcatggag gccggcctgc ctgaaggcgt ggtcaatttc ctgccggggc
     8641 agggatcagt catcagttca gtggtgatga agcaccccga cctgggcgga attcacttca
     8701 ccggctccaa cagcacattc aatgcgctct ggaagactgc cgcaaccaac ctcgataaat
     8761 acaaatccta tcccaggatc gtaggcgaaa caggcggcaa ggattacata atgattcaca
     8821 actcggccaa tccccaggaa gtggccacag caatcatcag gggatcattc gagtaccagg
     8881 gacagaaatg ctccgcctcg tccagggttt atatcccccg ttcactctgg ccggaaatct
     8941 ccgtgcatgt caaacggcag gccgaagaga taaagatggg cgatcctgct gacttcagga
     9001 atttcatgaa tgccgtcatc gacgaaaagt cgtttgacaa tatcatgcat tatattgacg
     9061 aggctgtgaa atcacctgaa tgtgagatat ttcatggcgg aaagggtgac aagagcaagg
     9121 gatacttcgt ggaaccgacc atcattctga caaaggaccc gcatttcatc accatgaaag
     9181 aggagatctt cggtccggtg gtaacccttt atatctacga ggatgacagg tacgaagaga
     9241 ctcttaggct tgtcaacgaa acctcacctt acgggctgac cggatcaatc ttttctactg
     9301 accgccctgc gatggtaaaa gcatgtcagg tgcttcgata tgcagccggc aacttttaca
     9361 tcaatgacaa gcctactggg gcgatggtgg gtttacagcc cttcgggggc gccagggcat
     9421 cggggacaaa tgacaaggcg ggcggcaggc ttaacctgat caggtgggtc tcgccgagga
     9481 ccatcaagga gacattcctg ccggcaaccg acttccgata tccgtacatg gattagaaag
     9541 tctgagggtc tgaaggtctg aaggtctgaa ggtctgaggg tctgagggtc tgagggtctg
     9601 agagtctgag ggtctgaggg tctgagggtc tgactacatt tgaattttat gaacaggcta
     9661 aaagaaaatt gttaaaaaaa cataaaacag ggagtacgcc gaagagatat ttcaggtaat
     9721 tttaggtgtg agttaatccc aagtttaacc taaacctgtt caaatgaaaa aaattctact
     9781 gtgcgctgcg gcagttgcat tgctgctcgg agcgtgtgag agaacacccg aacccttcat
     9841 tcaggaggat gattacatta atacctctct cgaggtaatc cctggtcagt atgtcgtttt
     9901 gctgaagggt gaattcggct atgtcaagtc aggcaccatg agttatgatg atgcccagct
     9961 ggcaatgact gccttgacga aagacatcct tgcaaaagcg ggaatctatg accgtgagcc
    10021 actgcaggta tattcaaaag caattcaggg atttgccgtt aacctcagcg attcagaggt
    10081 tgctgctctt gaaaagaacc caaacgtaaa gggcatctgg cctgatcaga tgtttaccct
    10141 tgcaaaacca gggacgaccc caaccccacc ggctgcacag gcgaccccgc ccgggataac
    10201 aagggttggc ggaccggtta attataccgg atcggcaaaa gcatggataa ttgataccgg
    10261 gattgacctg gatcatcctg accttaatgt ggacggaggc agtggcagga acttcatttc
    10321 aaccctcaaa acccctgatg acgataatgg tcatggttct cattgtgcag gtattgttgc
    10381 tgcaaaaaat aacactattg gagttgtcgg cgttgctgca ggtgctaccg tagtgccggt
    10441 aaaagtgctt gacagacgtg gctcgggagc atattcggta atcatagcag gcgttaatta
    10501 cgtggccgga gccgccacac ccggagatgt tgccaatatg agccttggtg gcggagccta
    10561 tgaacctatt gatctggctg ttataaatat ggcagccgct ggtatccgcg ttgccctggc
    10621 tgctggtaat gagagcgata atgccaacaa tcactcacct gccagggcta acggggtaaa
    10681 catttataca acttctgcct gtgatagcaa agatgtctgg gcttacttct caaattacgg
    10741 taatcctccg attgactatt gcgccccggg agtaaacatt tactcaacat ataagggtgg
    10801 cggttataca accatgagcg gtacctcaat ggctgcaccg catgcctgcg gagtactgct
    10861 ggtcaccaac ggaaatccga aaacctccgg ttatgtgaag aacgacccgg acggcgacgc
    10921 tgacccgatc atacacaatt aatcctcagc tgcacacaag aaaccctccg gcatcccggg
    10981 gggtttttta tttgcgtagt cagccggcag aattctttat gctgcattcc ttcattgcag
    11041 aacagggtgt ctcaccttcc ggccctgaca aacctgacag ccctcccgtc accggtcaca
    11101 agtatataaa tgcctgccgg gaatacgctc acgtcgatct ccgtcatatc agttcccgag
    11161 aacagcatga cgcctgtaat gctgtaaatc ctgcagtcgg cagcttcact taaccataaa
    11221 gtttctccaa tgacaggatt gggatagatt ttcactccca tgtctgcata agtgctaccg
    11281 gcagaggtga tgttattgga ccttccggga gtggccgtgg caagaaaaac aatctcctca
    11341 gccccgtcac tcttcctgcc ggtgctgata tcctcctgct gaaggccgaa agagacagtg
    11401 tcaaccataa gtttattaag cgtatatatg cctatctcct ccccttcctt gtcaagttta
    11461 aatggcgcat gcttctctcc cagcccgggg atcccatcag cccagaagat cgtaaagcct
    11521 ccggcaggaa gcactacaga gggcatcctc catttcagcg ggtcattgaa attgtctgtc
    11581 aggcaaattt caccgagatc aacagcttca ttaccgccat tgtatatttc gatccagtcg
    11641 ctgtagtatc cgtgctcatc tgccacagtc atctcgttgt ctgccatgaa ctcattgata
    11701 aacagcagtg gtgtttcacc gccaacagga gctgcaagtg cggcgacaca cggcaacagg
    11761 gacatctccc cgtctccccc tgtggcctgc acccggtaag ccacctcatc ctcatcagga
    11821 attccggtga gggtgacgct gtatcttccg ctgccggaag tgctcatctc tgcttcaagg
    11881 gaagtgcctc cggcgacagt atagataagc ttcacggcag cggaggctga gattgcctcg
    11941 acggtacagc tgatatacag cggctctccc gccttccggt catgtgcgat gtattttatt
    12001 acaggatcag gatcagcctc ttcagcctga gaagccatcg aggccaatct cacctgaagg
    12061 taaggataaa tgccccattt gacgtgggcg ccaaaggctg aagtaaaaga attcaggaag
    12121 tcgctgtggt caaatccgta gtcccgggga taataaggat cattggcaat atatggtgag
    12181 atcatttcct tcctggcatc aacagactgt atcagggagt ccaggtccag cacaccgccg
    12241 acaagccggg ctgcatagaa gctgaattgc tcacgcaaaa ccggattatt gattatcctg
    12301 tcgtacagcg gcctttgctg gtcgcctccc ggctgccagc cgtacatatt ccgcgtggcc
    12361 cagtcaatcc cgaaccagtc tattccgaag gtattgtcaa cgtcataggg aatatattcc
    12421 agcctgcctg tcagggtgtt gtgatagagg taaaagttgt tcttgttgta gatgtatccg
    12481 tcccagtcgc cgcataaaat ctgcacggcc atgattttca ggtagtcctg gacgttgaag
    12541 accctgtcga gctcgcacag gagagcctca ttgccagtgt tgtgcaaaat tccgatgaac
    12601 ccggcgaggt ctgaaaagtc attttcttcc tcgttggtat tgagctcata gatccagcgg
    12661 gagccgttcc atatcctgta actgtcaggg tcatcacccc tgtagttcag gtctgccggg
    12721 tagaggcact tgtagagatt cccgtcctta ttcccgaacc ttgaaagcgc aaactcctcg
    12781 tcaatgtgct ccacattgat gtaaagtccg taatagttcc cgtttatgta aaccctgaca
    12841 tggtttgccc gcggggcggg aacatcccat ttgcggagga tgtcccacat tattttcgag
    12901 cgcataacag agggatcatt gtgttcgcca ttgaggttca tcttctccac accgtggtac
    12961 ttgccgccat cggtgaaggt gttgaaggag accttgaacg actttttctt tgagtcacgc
    13021 gaggtgttcc cgcgaagcct gaaacctgcc ggatcaatgg tatcacgcac agtgccgttg
    13081 tcaaagacaa atactgacct gaactccctg tcactgtacg gattggcata aagccatgcc
    13141 agtgtgtccg gattgatggt tatgtatacc acggggacca cagtgtcagt gaacagttgc
    13201 ccgttatccg gtaatgaggg ctgtgaccgg ctgcctgtaa caactgtcag cagaagcagt
    13261 atgcaggcga gtgctttttt catcatgcaa tattactcaa tttaatacct ttatgggtcc
    13321 taacccggtc atacagcaat gagttcaaaa ccactgagat ttaccgacgg agtggtcaca
    13381 atccgcaaat tcaggcggag tgacaaattc cgcatggcag agatagccaa caatgaaaag
    13441 gtggctgcca acctgaggga tgcattcccc tcgccctata ccctggaaga tgctcagaaa
    13501 ttcatctcga tgtgtctcag gcaggtgcca tacgggatat ttgccattga atttgagggg
    13561 gaatatgtgg ggaacatcgg cctgcacagg cagggtgacg tttatcgcaa gacggccgaa
    13621 ctaggctatt tcataggaga accttactgg cataagggaa taactccacg ggcagtgaac
    13681 ctcatctgcg aatacggctt ccgcgaactg gatgtcatca agatattttc aggggtgttt
    13741 tcattcaaca ccgcttcgca gcgtgtgctg gagaaatgcg gctttaccct ggaagcggtg
    13801 ctgaaggctg ccgtgataaa gaacgggaaa atctgcgatg agtaccggta tgcccggata
    13861 ctcgacggat ataaatgatc agagcctttg ccggctggcc ggatgcccgc taaaggctgt
    13921 ccagcgcctt cctaagtttt tcacctacct ccgcagcaac ctgatttact gcctcgttcc
    13981 ggacaactga catcgcctct gagggattaa ctgcagctat ctccgtttcg ccttcgccct
    14041 gctctattac aagcacattg catggaagca tcaccccgat cttgtcttcg gcctgcaggg
    14101 ccctgtagga aaaggccggg ttgcatgccc cgaggatacg gtagcgcttg aaatcgaccc
    14161 cgagcttgta cttgatcttc tcatgcagat cgacttcaga tatcacaccg aatccctcgg
    14221 cggaaagaga ggcggtaacc ttttctactg catcatcaaa tgatgtcctg agcgttttgg
    14281 agtaataata tgccattgtc ttcggttttg cttttcaatc cggtttaaca aacagtaaac
    14341 aaagatatgg cggattggtt cgaaaaaaaa tctgatccgg tgccccgcgg taagttgcaa
    14401 atatcaatga tgtaggtaac tttactatca ttctggcctc tttcccggat aataagaaaa
    14461 accatgaaga caattcctga aagtctttta acctggttgt tttgcctgat ctgtattgca
    14521 tgttcagaac gccgggactc agggaatttt atcgaaacct tcaacgctga tttcagggca
    14581 gaactggccg gcctgcatgt gtcgaatgct tcatatgcgg tcttctgcac tgactcggta
    14641 atactgcagg acagcttcag cgccgccgga accggaaccg acggcgactc accattcctg
    14701 atcgggtccg tcacaaaggt cttcaccgcc gttgccatca tgcaactgta ccaggagggg
    14761 aaaatcgaca ttgacaggcc tgtctcggat tatgtcccgg attttcagat acggcagcgg
    14821 ttccctgaat cagcccccat tacactgcgg gctgttatga cccatcatgc gggaatcccc
    14881 tccgacaact accttcataa attcagcaaa atgccgaatg acttcattga ccttcccggt
    14941 tacctcaaca ccctgtacac ctgttacccg gtcggagaaa tatgggccta ttcaaatctg
    15001 ggttatgcat tgctgggtgt ggttattgag cgtgtaagcg gcatgaggta cggggaatac
    15061 atcgcccggc acatctttga acctcttggg atgaattcat ccggtctcta ttctgattat
    15121 ggttcgcaga aaagtctttc cgcagcatac gatggcaaag gtaacctgac aaccgaactg
    15181 cccatatgcg ataaaccggc aggcgccata tattcaacgg taaccgacat gatcaggttc
    15241 ggacgtgcgt ttatcgacgg gaaagagata ctgctgagaa aggagttcct ggatcagatg
    15301 tttgagctgc agaacgctga caacctcctg gaccttgacc acaggtcggc cctctgcttt
    15361 aacttcaaga acaaagcacc ggaactcggg aggctgctcg aacacggcgg tgccaccatg
    15421 taccaccgtg cacagctcac aatagcccct gatgcaggcc tggcctgcgt cctcctctct
    15481 gattccccga acggaaagga taatgcctgg agacttgacg aacagctcat ggttgaatat
    15541 tgcaggtcgg caggtatcac tcccgacagg agatttaacc gtgaaaagaa aatgctgttc
    15601 acaccggtaa gcagcaagaa cctgcagtca tttgcgggca actatgccat gcccggactg
    15661 gtgtgcagga tcgattggaa acacaacctg ctttccccga caatcgacgg acagaacttt
    15721 tacctggctc agcacgactc cggttccttc atacccgcaa agtggttttt ggggatgttg
    15781 ttcaaatcga aacggatgta tttcctcctc gaggagataa acggagaaaa gcacttcata
    15841 caggtgatgc catggggagg gttgaatatc atcggtaccc gggtgacccc ggaacctgtt
    15901 tcggagacct ggaaaaacag ggccggtgat tatgtcgttt caaatgccgc tcccggcgat
    15961 gcccctacga tagaaaaaat gagcatctcg gttgaagaca gctttgtcgt tctcaggtac
    16021 ggattcagcc cggaaatgag tttcgggcag aatgcctcgg ttgcactgaa cctggtgaac
    16081 gacaatgaag ccttcgtgct cggctatggc cgcggaggag gcgagagtgt gatcttcggg
    16141 cctgacggca gaagtttcag ttatatggga ttgaagtttg tgaaaatatg aagaaaagta
    16201 cttttttcgc gattcttgtg gctttcgcgt tatgcgcagg agcaattaaa ggccagaaac
    16261 aggttaagca tcagaacgac tggcaacttg tctggcagga tgagttcaac ggaacgcgcc
    16321 ctgacacctc gaaatggaac gtactgatac gggagaccag caagcacaac gaactgcaat
    16381 actatgtgcc tgacgaggtt tacgttgaga acggcatcct gaggatccgg agccgggtgc
    16441 gcaggtacgg ctcaaaggag tacaccagcg gcaggctgga tacggaaggt aaatttgccc
    16501 ctgtctacgg tcgcttcgag atacgcgcca aactccctgg cggacagggc ctctggcctg
    16561 ctcactggct ctacccacag gagcgcgact ggctaatgga aaagaccatg gaagatgccg
    16621 ttgccgccgg aaaggaacga ctcatcccgg aagagagacc ctggtatacg gagatagata
    16681 tcatggaatt cctcggccat gaaccctctg tcctgtacgg cacaatccac tactgtgcat
    16741 ttgacggcac caggagatca agctccggaa tatggagggg caccgttgac tatacaaagg
    16801 attttcacac ctatgtgctt gaatgggagc ccgactccat ccgctggttc atcgacggca
    16861 accggataca cgcaactgcc agcggcattc cccataagcc acattacctg atacttaaca
    16921 ccgccgttgg cggaggctgg ccgggaaatc ctgactctac aacggtcttt cctcagtacc
    16981 atgacataga ctatgtcagg gtttacgcaa aagagtcata cttcccgcag caataacaaa
    17041 atcccctgcc ggttgttata tgatccggaa ccataatcac caggcggtga gcggaaaaca
    17101 tgtcagaaac cggcaaacat aaggaaccgg cgccccaaga actgcagcag ctgctcacgc
    17161 tgctcaggac ccgattcatg aagaatcctg cgcgccataa gggaattgag tgggaaacag
    17221 tgcagcaaaa gctggaatcc aaccggctga aactccggtc gctttttgag atggaacgca
    17281 ccggcggcga gccggatgtc gttgccagtg atagtacgac aggtcagatt gttttcaatg
    17341 attgttcacc cgagaccccg aaggagcgcc gtaacctctg ctatgaccgt gaagcacttg
    17401 attccaggaa ggagttcaaa ccatccggca gcgccacaga catggcagca gccatgggca
    17461 tcgaactcct cacggaggag gaatatctcc atcttcagac actcggcgat ttcgacacaa
    17521 aaacatcaag ctggattaag acacctgcag acataaggaa gctcggcgga gcaatcttcg
    17581 gtgaccgccg cttcggacga gtatttatct atcataatgg cgcatcatct tattacgccg
    17641 tcaggggatt ccgcggttca ctgagggtct gaattccggt ctgtgttgcc ggatggtcat
    17701 ttgcctgcgc caattgcttc tttctggtac aattattgtg gcaaactcac tgacccctaa
    17761 ccgttaatga aatgaaaaga atcgtaattc cacttttact ggttctcaca gtttcctgcg
    17821 aaatgctgtc agattgcccg aaactggtta aaggaaaacc ggaaggccac ctggtcgatg
    17881 actcggagct gaaaaccatt aaaacactct tccgggcaag caatctctca tatgagaact
    17941 atcagttcta cagtctcgag tgtatcctct cgggtgagtt ttatgtcagc tgccaccagt
    18001 atgtcaatga cctgaaagta tggaccgacg acctgttctt ccatttcgac aaaaacggaa
    18061 gattcgagag tctctcggga gatattgttt ccgccatcga tgcaggaaca tcaccctcca
    18121 tggatgtcac gcaggctgaa tcgcgctttc tcgggagaat gaatgaggac agcttcttcc
    18181 gcaacagtaa aagtgttgcc aacgggtgca ttacctgtga actggtctat tatgacattg
    18241 ccggaatgtc aggcggatca actccggatt atgttctggc ctggcttgta aaaccggaaa
    18301 aatcagatta cccggttgcg gtcgtaaaag attcggacca gtcggtactg tattacaata
    18361 acgggattat cttctagaac gctcccgaaa atcttcaccg ggaagagtat tacaatctca
    18421 tgaccaggaa ctactatttt caggatattg tccggtcccg gacagcaacc tttgatattt
    18481 tcggggttgg cctgaagaag catcacatct cggcgatgct tgagtttgat gtcaccgaaa
    18541 gcaggtccgg gctcagggaa ctcagaaaaa agggtatcag catctcattc aatgcctggc
    18601 tgataaaagt gatcggaagt gtgattgcaa aacaccccga agctgctgct tatttctata
    18661 acaaaaggaa aatcataatc ttcaatgaca tcaacgtgtc tcttatcgtt gaaaagaaga
    18721 taggtgacgc aagggttccg ataccccttg ttatagaaaa ggtgaacaaa aaacctgcgg
    18781 cggagatctc ggaggaaatt gatgctgcca aaggccggga gctgagcagt ggtgatatag
    18841 tcctgaagaa aaaatcatca gcatacgaga ggttttatta ccgaatgccg ggcttcttcc
    18901 ggagattctt ctgggtagta ttgctaaaaa ggccgaaagc agccttcaga acgatgggaa
    18961 acgtatctgt cacatcagta ggaatgatag gtaaggtcaa cgggtggttc attcataaat
    19021 cggttcaccc catctcgttt ggagtcgggt cagtgctcat gaagcccgta gtcatcggga
    19081 atgagataaa ggccagggaa gttttgaata tgactgtgct cgttgatcat gatgtcattg
    19141 acggtgcacc catggtcagg ttcctgggtg atctgacaga cttcatcgaa aacggaaagg
    19201 agatggactg agcacgaaag cgggtgtgag ataaaagaaa aagagtgctc gctctcaatc
    19261 acactctctt tcttttgttg acccacaggg gctcgaac
//
LOCUS       DAOPXU010000049        19172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_2988_length_19172_cov_11.846786, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000049 DAOPXU010000000
VERSION     DAOPXU010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19172
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2988_length_19172_cov_11.846786"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..762)
                     /locus_tag="PKK03_06590"
     CDS             complement(100..762)
                     /locus_tag="PKK03_06590"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX84105.1"
                     /translation="MKRKLLTITAVLVIACSAMAQAPQLEKGNILLGVTSTAIVEGSW
                     GSDLMSLGIMRTKYTSGTDSYNENKVRSFSLIPKGGYFIIDNLSAGLGLLFVRSSSQH
                     VDSQGKLTETSFAAGPWARYYYPLEKVYPFAELEVMAGTCVAKMPTSGPDPDVDRYSL
                     FSLGVSLGVAVPLADRVTVDIMAGYIRTVWGSTQYYEGEETDLREIYAGPAIKVGFSV
                     FL"
     gene            complement(775..1416)
                     /locus_tag="PKK03_06595"
     CDS             complement(775..1416)
                     /locus_tag="PKK03_06595"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX84106.1"
                     /translation="MKRTFLTVAVFLLGIATITAQPQLEKGNLLLGATSTVALSTSAS
                     SELIGLGFIKATYKYGSGPAEDAFRRTSFNILPRAGYFVTDNLAAGLQAVVFGYTEKD
                     LEYDDTYKESTIGIGPFVRYYYPLDRFYPFAEAEFLFGTVKEFWYDEDEKTPFIMFGV
                     MVGAALPLGDNVTFDAAAGYLRSSLKNTDAETEDEVHFITSGFCLRMGFTIYL"
     gene            1668..2630
                     /locus_tag="PKK03_06600"
     CDS             1668..2630
                     /locus_tag="PKK03_06600"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX84107.1"
                     /translation="MIREIKVQDLNTEKFIAEKVSEIRAAVGEGTAINALSGGVDSSV
                     VTMIGHLALGDRLRTVFIQNGLMREGEAEAVASVFARLGVKVEIIDAQNEFLEALKGI
                     TDPEQKREAITQTFYKKVFGRIVRESGARYLLQGTILTDIDETVAGIKRQHNVFAQLG
                     IDPQETFGYHILEPLIQLRKDGVRMTGRAAGLPEEMFNRIPFPGPALAARIIGEVTQE
                     KLDIVRKATVVVERLLAGHGAFQYMAILHDDRVTGMRDGKRDFGRQIEFRCWDSIDAR
                     TATPTRLPFELLEQLAAKILDTVPGVVSVTYNIATKPPSTIEAV"
     gene            complement(2702..3991)
                     /locus_tag="PKK03_06605"
     CDS             complement(2702..3991)
                     /locus_tag="PKK03_06605"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNX84108.1"
                     /translation="MTYQETVEYLFSLLPAYHRVGKPAYKGNLDNTLELDRYFSHPHS
                     RFRSVHVAGTNGKGSVSHMTASVLQEAGYRTGLYTSPHLKDFRERIRIDGEMIPEAEV
                     VRFVADHDGIIGKMKPSFFELTVALAFDYFARMKVDIAVVEVGMGGRLDSTNIITPLL
                     SVITNIGHDHMEFLGPTLKAVAGEKAGIIKEGVPVVIGETQPETRDVFLQRAEAMHAP
                     VTFADSRFRCRLGRIFPDSSMRRFTLSGPDDGMTLKGETPLGGLAQRKNLQTTAAAVE
                     KLSADLGLSREHFLRGVAGVVENTGLMGRWQILSRSPLTVCDTGHNREGLEYVMRQVR
                     AAEKENLHMVIGFVNDKDLGSVLPLFPRDARYYFTRASVPRALDENLLKAEAGRYGLN
                     GETFHSVPVAVDAARTAAGPRDMIFIGGSTFVVADAL"
     gene            4119..5456
                     /locus_tag="PKK03_06610"
     CDS             4119..5456
                     /locus_tag="PKK03_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNX84109.1"
                     /translation="MAKKKKQKKIFILDTNVLLHDFTCIYNFEENDVIIPIVVLEELD
                     KFKRGNDMINFHAREFTRELDKIAGDMLLTGNIPLGENLGSLHIETGKQFSERVSESF
                     PERTPDHRILAIAEFVTQENKDKTVVLITKDINLRMKAKSLGIRSEDYQNDKVANLDD
                     LYKGIRIIENIDHDLINKLYEKADGVPATELKIKDTEPGHHYFILRNSNSSALAHYDP
                     ASKMLLRVLKQTTYGIDPRNAEQTFALDALCDPDIQLISLTGKAGTGKTLLALAAALH
                     QHKRYKQIFLARPIVPLANRDMGFLPGDVKEKMDPYMQPLFDNLTVIKHRFSAQSPEF
                     IRINDMIKDEKLVITPLAYIRGRSLSNIFFIVDEAQNLTPHEVKTIITRAGEGTKMVF
                     TGDIEQIDSPYLDSGSNGLSYLSDKMKGLDLFAHVHLVKGERSFLAELASKLL"
     gene            5504..6811
                     /gene="mtaB"
                     /locus_tag="PKK03_06615"
     CDS             5504..6811
                     /gene="mtaB"
                     /locus_tag="PKK03_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNX84110.1"
                     /translation="MKRKVAFHTLGCKLNFAETSTMARTFPDDKFERVSDDSHADIYV
                     INTCTVTDTADRKCRQAVRKFVHRNPDAFIAVVGCYAQLRREEAASIEGVDLVLGTYE
                     KFDIASYIDNLQKRTAPEIHSCETVDTDGFMTSWSAGDRTRSFLKVQDGCDYHCSYCT
                     VPLARGKSRNPAVSEIVAAAEKIVAGGVMEIVLTGVNVGDFGKSTGESLGQLLESLTK
                     VNGLQRLRLSSIEPNLVTDRIIDIISEGKVLLPHFHMPLQSGNDRVLGLMRRRYRREV
                     FRDRVMKIRERIPDAGLGADVIVGFPGETEEDYADTYTFLESLPLSYLHVFAYSPRPG
                     TPAAEMPGAVNRQEKEKRSRQLIRLSESRRMQFMRNASGEVHEVLFEKRGHDGTVAGL
                     TGNYIRVMTPWVKGLPGSIRQVRLTTLRDDSSMNCELTDKEAQ"
     gene            6808..7689
                     /locus_tag="PKK03_06620"
     CDS             6808..7689
                     /locus_tag="PKK03_06620"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNX84111.1"
                     /translation="MKRAGYYIFVAMSYTITLLPMWALHLLSDLLWPFFYHVVRYRRR
                     VVETNLRNAFPEKSPEERKKITKRFYRHLTDMIVETLKAMHMSAEQIKKRFSVTDTSL
                     ADRFYSEGRDLVALCSHYNNWEWYSSMQLSSKHRAVAIYKPLKNKDFDRFLYKIRTRF
                     GVWVTPMNHIVRDLVKCRSEKILTMSGFIADQTPPPDDNAFWTTFLNQDTGFYRGAEK
                     VAIKYDMPVIFINIVKRKRGFYELEYSLISEHPREEEPNAIIARYAARLEEVIRAKPE
                     YWLWSHRRWKHKRPEKK"
     gene            7686..8711
                     /locus_tag="PKK03_06625"
     CDS             7686..8711
                     /locus_tag="PKK03_06625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX84112.1"
                     /translation="MIRTAVVILNWNGRKFLEQFLPGVVKNTLAPDTSVIVADNGSED
                     DSLLWVTENHPEVEVIRLDNNYGYAGGYNRALKEVEAEYYILLNSDVDVEPAWAEKLI
                     TFMEMHPNVGACQPRIRSYSNRDSFEYAGAAGGYIDRLGYPFCRGRIFDTVENDHGQY
                     DDVREIFWASGSCMVVRGAAFDQCDGFDESFFVHMEEIDLCWRMQNAGYTISYFPHSV
                     VWHVGGGTLGYGSPSKIYYNFRNSLIMLAKNLPAKHLRRTILTRQVLDGLAAVMLLFT
                     EGRAAAAAVVRAHRDFRRQRTAIRTFRTLTPNPGIFHTPHTTMNKYLLYEYYIRKRKT
                     FTELKWT"
     gene            8726..9868
                     /locus_tag="PKK03_06630"
     CDS             8726..9868
                     /locus_tag="PKK03_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNX84113.1"
                     /translation="MAIFLTVVLTVYILTNLYLWFKGRRALSGAGINTGWYNAIFIAL
                     ASAFVIGKFLENSFTSVFTDIVNVIGGFWMAFMLYGFLAWLAADILMLLQKPFHLIPP
                     DVIPKLRLWLFAGITSVTLLLIIIGFINAVSPVTSRYAVEVSKKLDSSHEPMRIVAVS
                     DIHLGSIIRKRSMRHLSEMITGEKPDLVLFLGDLLDGSIGPVLRGDLLSYLSIPAPRF
                     GTYAITGNHEYMSDLVKSVPYIESKGIRVLKDEIISLENGVQIIGRLDRTAMHSQGIG
                     RKPLEQLLAETDTTAPVILLDHQPYDLSALASTPVDMQLSGHTHNGQIWPLNLITDRM
                     FELSHGYRVFGKAHVIVSSGFGIWGPRMRIGTRPEILSITLTGKSE"
     gene            complement(9860..11152)
                     /gene="murF"
                     /locus_tag="PKK03_06635"
     CDS             complement(9860..11152)
                     /gene="murF"
                     /locus_tag="PKK03_06635"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNX84114.1"
                     /translation="MEIERLYGLYRNSAGVTTDSRAVKKGEIFFALKGPAHDGNRHAA
                     AALEAGALASIVDDHSLSGERIIVVDDVLEAMSALAACHRKRLQMPVIGITGTNGKTT
                     TKELLTAVLSRRGRVHSTPGNFNNHIGVPLTLLSAPDDAAFLVVEMGANHMGEIAALC
                     KVSMPTHGIITSIGTAHIEGFGSMENIRKAKSELYQSLRESNGTAIYNDDNPVLCELV
                     SGMTNGVPYSAPGGHTLSAAPAGDDLMLLKVKAVIDGKEHYFETGLFGSYNIDNVRAA
                     MAAGLLFGVPEDEIIDAISSYRPSNNRSQVTETGRNTVIRDAYNANPSSMENAIASFA
                     ALKADKKMVILGDMLELGSESAHGHAAVIRQLRNIGDIRAVLVGPLFREAAADFPATL
                     FGTSEAAAEWLKSERPEGYTILVKGSRGIMLEKVFPLL"
     gene            complement(11218..12705)
                     /locus_tag="PKK03_06640"
     CDS             complement(11218..12705)
                     /locus_tag="PKK03_06640"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNX84115.1"
                     /translation="MYKLRALPVFVVVALFLFSCKGAKQDNVSPTTGWSYRDPKSSDV
                     NDLGYPIGEASEQVTGPNLVLIEGGTFTMGRVDQDVMFDWNNSPRRVTVSSFYMDETE
                     ITNLDYREYLYWLRRVYSDYPEVHRRALPDTLVWRSPLALNEPYVQYYFRHPSFNNYP
                     VVGVSWLQANDYCVWRTDRVNEQILLDSKWQDANVEQSGANNFNTESYLAGQYEGVPG
                     QRVDELHPRVRQEDGILLPRYRLPTEAEWEFAAYGLRGNTFEERIYERAIYPWDGHNV
                     RNAAPKYRGEFMANFVRGRGDMMGVAGNLNDAGSITVDVRSYWPNDYGLYCMAGNVNE
                     WVMDVYRPLSSEDVDMFNPFRGNVFTDLRRDANGDVVEKDRLGRLQIDTVKPEDVAHR
                     YNYQKSDYRNYLDGDVISSISSSLEWSGNADLPQTERMYSQNEVDNDYVTLINDRARV
                     FKGGSWKDRAYWLNPSTRRYLDQEASRDDLGFRCAMIRVGSPSGL"
     gene            complement(12781..13746)
                     /locus_tag="PKK03_06645"
     CDS             complement(12781..13746)
                     /locus_tag="PKK03_06645"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNX84116.1"
                     /translation="MKLRYRTLLAALLICPLTLSGQDTWYGSVSGMQVMHNPAFAGVE
                     STSTVKISAFSFLPGNGFGLRSVYASYDGYFSSLRGGAGAWVSDDMLGDIMNDLRGGA
                     FYSYHFRAGRRLFVTAGLTAGVITRGIRSGSVILPGDIDPFRGITGGGTDYIPPAPIT
                     RFDLGTGFTVSSGDWFAGASIMHLTRPSLSDDALEHNRVNRLLTLTGGISLTPGGKDF
                     SLLPSAALLIQGDETKIYLGSEASWKGLAGSLSLWHVTNGFTALGASAGWDATSVKIM
                     ISYSYIVAGGITSLGGTAMVKAGAIFSFGNVEKSRGAHIIKLPLL"
     gene            13852..17331
                     /gene="porU"
                     /locus_tag="PKK03_06650"
     CDS             13852..17331
                     /gene="porU"
                     /locus_tag="PKK03_06650"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HNX84117.1"
                     /translation="MMKSVATGLLVMASALNAASQEYSASSVPAAGASGRASVSVQGA
                     AEQEYSASSVLGNGQWVKIAVTEEGIYRLDYSRLKEMGFTDPSSAVLYGNNAGQLSFM
                     NDDTAPDDLKKIAFRAEKGTDGVFNEGDYLLFYAEGTHRWIADPGRRGSRFVRHHYSD
                     TAWYFITSQPGGALTVQTETPPAASPSIISSSTDLYMRHEREEINLIRSGREWYQQVV
                     PGTQNNIGPGFTDLPAGGKIHYTIRVAARSDTETSFILRQGGETIKTITVPAVTMTDI
                     NGVYAATMTVSDSIVSSSGSPAFSLAFSSGGNMAATGYIDYADFTARSSLVWRDRNLF
                     ISDSRSVGTSAVTRFTVEGSSSLSIWDISNPAAPLTIQTTPSSGNTVFVAATDSLRRF
                     VAFSLAQVKQPLRSVRVPAQNLHAMMPAGMIIVTYPLFMPYAKKLAELHLADDGLTSL
                     IVTPEQVYNEFSGGVPDATALRNFIRMIRERGMQSGQQTRYLLLFGDGSYENKTPPPG
                     NSSFIPTWQSVNSTTGVLSFTSDDFYGLLDDGEGEADGFLDMGIGRLPAYDTASAGIM
                     VRKISSYLQGSNQGSWRNILCLVADDEDSNLHMFDAEGLAEAATTAAPPLTVEKIYLD
                     AYRQMTSVSGDSYPDASRAVDDRMAAGCLIMNYVGHGNESGLAHERVIRTGNINSWKN
                     SRMLPLFITATCEFSRFDDVEINRASSSITARNSAGEMVLLNPDGGGIALMSTTRVVY
                     SAPNYTLNRAIYDYAFRTDAEGRSMRLGDIIRQAKVSSGTGMNKRNFLLLGDPALRLA
                     WPSTGKVVTDSINGIHVSMPFDTLKALSLMTVSGHLEDAAGNLMTGFNGTVEPAVFDK
                     PGHVSTLANDGGSPITFPVDGNVLFRGKTNVTGGRFSFSFIVPLDINYSYGSGSVKYY
                     AYNGATDLNGSFTGISVGGFSDAASDDTEGPQIRLFMNDTLFNDGGVTDASPVLLALL
                     SDRSGINATGTGIGHDIIAWLDDDMSEAVVLNSLFRADVGRHISGSLAYPLLVTGQGK
                     HTVSLRAWDNLNNPTVATLRFAVETGGTFRLTDLLAFPNPVTDGTKFTAGHNRPGTEI
                     DLTITIFSSDGRAMKVLRNKSSGAGYALPDIPWDGCDENGGRVARGLYLWRAEAVTAD
                     GEKTSATGRFIIL"
     gene            17396..18559
                     /gene="porV"
                     /locus_tag="PKK03_06655"
     CDS             17396..18559
                     /gene="porV"
                     /locus_tag="PKK03_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HNX84118.1"
                     /translation="MKNKALTVSIILLFSIFVSPAWAQDDDPYNVIQTAVPFLTIAPD
                     SRAGALGDAGVATSPDLFSMHWNPAKYAFIDGNGGFGMSYSPWLRALVPDINLAYLSG
                     YYRVDSRQVLAASLMYSSLGEIQFTDDYGNYQRNYNPNEFSVDVAYSRLFSEHWSGGV
                     AMRFIYSNITGGMTVGTTETKAGIAFAADASAYYTNKLKLGSRDGNIAAGINLSNIGS
                     KMSYTTDVDPDFIPMNMRLGTTFSINLDRYNAIGFSLDVNKLLVPTPPVYDEESNIID
                     GKDPNVSVPVAIFQSFYDAPGGFSEEMKEFSQSFGIEYWYNQQFALRTGYFNEAQSKG
                     NKKYFTVGAGFRLSIFSLDFSYLVPITQNNPLARTLRFSLGFEFDSLRNLSQK"
     gene            18568..19068
                     /gene="ispF"
                     /locus_tag="PKK03_06660"
     CDS             18568..19068
                     /gene="ispF"
                     /locus_tag="PKK03_06660"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNX84119.1"
                     /translation="MTLHQRIGQGIDFHRLEEGRELWLGGVRINSAKGCVAHSDGDVL
                     IHAICDALLGAAGLNDIGYHFPDTSEEFRGIDSKILLKRVVELITGAGYRIVNIDSTV
                     CLELPKLAPYITAMRTTMAAIANISPDAVSVKATTTEKMGFTGRGEGIVALAICLLAD
                     LIPDKV"
BASE COUNT         4780 a         5214 c         4814 g         4364 t
ORIGIN      
        1 ctgccacaga ggggctgaaa gaattcatac acccggacag atgaggctcc ggatgacaat
       61 gactaaagtt atttctgcca ggcagtttat ccagccattc tacaggaaga ccgagaaacc
      121 caccttgatt gccggtcctg catatatctc acgaagatcg gtttcctccc cctcataata
      181 ctgggtactc ccccacacag tccttatgta acctgccatg atatctacag tcaccctgtc
      241 ggccagaggt acggccacac cgagtgacac tcccaggctg aaaagtgaat accggtcaac
      301 atccggatca ggacccgaag tcggcatttt ggcgacgcac gttcctgcca tcacctccag
      361 ctcggcaaat ggatagacct tttcaagggg atagtagtac ctggcccagg gtccggcagc
      421 aaaggaagtc tctgtcagct tgccctgtga atcgacatgc tggcttgaag atctgacaaa
      481 cagcaacccg agcccggctg agaggttatc aataatgaaa tatcctccct tcgggatcag
      541 gctgaatgac cttaccttat tttcattgta ggaatcggta ccggaggtgt atttggtacg
      601 catgatgccg aggctcatca ggtcggatcc ccatgatcct tcaacgattg cggtggaagt
      661 aacccccaga agaatatttc ccttttcaag ctggggcgcc tgtgccatag cactgcatgc
      721 aatcacaagg actgcagtta ttgttaacag ttttcttttc atagttccgg cttattacag
      781 gtaaatagtg aaacccatcc tcagacagaa tccgctggtt atgaagtgga cctcgtcctc
      841 agtctccgcg tcagtgttct tcaatgaaga gcgcaggtaa cccgcggcgg catcaaaggt
      901 cacattatcg ccaaggggaa gtgcggcccc gaccattact ccgaacatga taaaaggagt
      961 cttctcatct tcgtcgtacc agaattcctt tactgtgcca aagagaaatt cggcttcagc
     1021 gaaggggtaa aatctgtcaa gaggataata gtacctcaca aaaggaccta tcccgattgt
     1081 actttcctta taggtgtcat catattccag atccttttcg gtgtacccga agactactgc
     1141 ctgaaggccc gctgccagat tatcggtcac aaaatatccc gctctcggca atatgttgaa
     1201 gcttgtcctc ctgaatgcat cctcggcagg tccggacccg tacttgtacg tggccttgat
     1261 gaagccgaga ccaataagct ctgaactggc tgatgtgctg agagcaaccg tcgaggtggc
     1321 ccccagcagc aggttgccct tttcaagctg cggctgtgcc gtaatggttg caatacccaa
     1381 aaggaaaacc gcaaccgtca gaaaagttct tttcatagtc cgtgttttag gtgtgcctac
     1441 tctttagttt ttcggcctct actccctact ctttaccggg attttcggga aactccctat
     1501 ataattgtgc ctgaggttat tgtttatcaa atttacacta cgggaaaggc gttgtcaaat
     1561 ttttctccgc aatggttgaa agagtgctga tttcttcctg gtcacacata cctcagggca
     1621 atcagccttg attatctttg catcaaccaa aaaaaaattc agggaaaatg atcagggaaa
     1681 taaaggtgca ggatcttaac accgaaaagt tcattgccga aaaggtcagc gaaatcaggg
     1741 ccgctgtggg cgaaggcaca gcaatcaacg ctctgtcagg gggagtggac tcctcagttg
     1801 tcactatgat aggccatctc gccctgggcg acaggctcag gacagtgttc atccagaacg
     1861 ggctgatgcg ggagggagag gccgaagctg ttgcttcagt attcgcaagg ctcggggtga
     1921 aggttgagat catagacgct cagaatgagt tcctcgaagc cctgaaggga ataaccgatc
     1981 ccgaacagaa gcgagaagcc atcacccaga cattctacaa aaaagtgttc gggcgcatcg
     2041 tcagggagag cggagccagg tacctgctgc agggaacaat cctgacagac atagatgaga
     2101 cagttgccgg catcaagcgt cagcacaatg tcttcgctca gctgggaatt gacccgcagg
     2161 agactttcgg ataccatatc ctcgaaccgc tgattcagct gcgcaaggac ggcgtcagaa
     2221 tgaccggccg ggctgcagga cttcccgaag agatgtttaa ccggatacct ttccccggtc
     2281 cggccctggc agcccgcata ataggcgagg ttacacagga gaaacttgat attgtcagaa
     2341 aagccacggt tgttgttgag cggcttctgg ccgggcatgg ggcattccag tatatggcca
     2401 tactgcatga tgaccgtgtg acgggcatgc gtgacggcaa gcgcgatttt ggcaggcaga
     2461 tcgagtttcg ctgctgggac agcatcgatg cacgcaccgc cacccccacc cggctgccgt
     2521 ttgaactgct tgaacaactt gcggcaaaga ttcttgacac cgtccccgga gtggtctccg
     2581 taacctataa tatagccaca aaaccaccct caaccataga agcggtctga ggatttgcga
     2641 tttgcgattt gcgaattccg aattactttc aaatcgaaaa tcgaaaatcg caaatcgcaa
     2701 atcacagtgc atccgccacc acgaaggttg accccccgat gaagatcata tcccgtggcc
     2761 cggcagcggt acgcgcagcg tcaacagcca caggcacgga gtggaaggtt tcaccgttca
     2821 gaccgtacct cccggcttca gccttaagaa gattctcatc cagcgccctg ggcaccgagg
     2881 cccgggtaaa ataataccgg gcatcacgcg gaaacagagg caggaccgat cccaggtcct
     2941 tatcattgac gaaaccgatg accatatgaa gattctcctt ctctgccgcc ctgacctgcc
     3001 tcattacata ttccagacct tccctgttat gcccggtatc gcacactgtc agcggcgacc
     3061 gcgacagtat ctgccacctg cccatcagcc cggtgttttc aaccacacct gctacccccc
     3121 tgagaaagtg ttcgcggctg agcccgagat ctgcagacag tttctccaca gctgctgccg
     3181 tggtctgcag gttcttcctt tgtgccaggc ctcccagcgg tgtttcaccc ttcagggtca
     3241 tgccgtcatc agggcctgaa agagtgaacc gcctcatgga actgtccgga aaaatccttc
     3301 ccagcctgca cctgaaccgg ctgtcggcaa aggtgacagg cgcatgcatg gcttcagccc
     3361 gctgcaggaa aacatcccgc gtctcaggct gagtctcgcc tattacaacg ggcacaccct
     3421 ccttgatgat cccggccttc tcgccggcaa ccgccttcag ggtaggcccc aggaactcca
     3481 tatggtcatg ccctatattg gttatgactg acagcagggg tgtgataata ttggtcgaat
     3541 caagccttcc tcccatgccc acctcaacta cagcaatgtc aaccttcatc ctggcaaagt
     3601 agtcaaacgc cagcgcaacc gtcagctcaa agaatgatgg cttcatcttc ccaatgattc
     3661 cgtcatggtc tgctacgaac ctgaccacct ccgcctccgg aatcatctcc ccgtcaattc
     3721 ttatcctctc cctgaaatcc ttcaggtggg gagatgtgta gagaccggtt ctgtatcctg
     3781 cctcctgcag taccgaggca gtcatatgcg atactgatcc ctttccgttg gtgcctgcga
     3841 cgtgcaccga acggaacctc gagtgcggat gactgaaata gcggtcaagc tcaagcgtat
     3901 tgtcaaggtt gcccttgtat gcaggcttgc ctacgcggtg gtaggccggc aacaggctga
     3961 atagatactc aacggtttcc tgatatgtca tgttgataac ctttatgcgg ttgttcgcaa
     4021 atttacaaaa agcgcttttc ccctacatac ggcaccgcta tctttgtcac cgtggtaata
     4081 tctgcgccgg agatgctgcc ctttaaacca aatctgttat ggcgaaaaag aaaaagcaaa
     4141 agaagatttt tattcttgac acgaacgttc tgctgcatga cttcacatgc atctacaact
     4201 ttgaggaaaa cgatgtgata atacccatcg tggtgctgga agagcttgac aagttcaagc
     4261 ggggcaatga catgataaat ttccatgccc gggaatttac acgcgagctt gacaagatag
     4321 ccggtgacat gctccttacg ggcaatattc ctcttggtga aaatctggga agcctgcaca
     4381 tcgagaccgg aaaacagttt tcagaaagag tctccgaatc attcccggaa agaacgcctg
     4441 atcaccgcat cctggcaata gccgagtttg tcacccagga gaacaaggac aaaaccgttg
     4501 tcctgataac aaaggacatc aacctcagga tgaaggcaaa gtcgctgggt atcaggtctg
     4561 aagactacca gaatgacaag gttgccaacc tcgatgacct ctacaagggg atcaggataa
     4621 tcgagaacat tgatcatgac ctgatcaaca agctgtacga gaaggctgac ggggtgcctg
     4681 ccacggagct caaaatcaag gataccgagc cggggcatca ttattttatc ctgcgaaaca
     4741 gcaattccag cgcccttgcc cattatgacc ctgccagcaa aatgctgctg agggttctca
     4801 agcagaccac atatggcatt gacccgcgta atgctgagca gaccttcgcc ctcgacgcac
     4861 tctgtgatcc tgacatacag cttatttccc ttaccggcaa agccggcaca ggcaagaccc
     4921 ttctggcact tgctgccgca ctgcaccagc acaagcgcta caagcagata ttccttgcaa
     4981 ggccgatagt tcctctcgcc aaccgcgaca tgggcttcct tccgggcgac gtgaaggaga
     5041 agatggatcc ctacatgcaa ccgctctttg acaacctgac ggtcatcaag caccggttca
     5101 gcgcccagag cccggagttc atacgcataa atgacatgat aaaggacgag aagctcgtga
     5161 tcacccctct ggcatatatc agaggccgca gcctctcgaa catcttcttc atcgttgacg
     5221 aagcccagaa ccttacaccc catgaggtga aaacaatcat caccagggcc ggcgagggaa
     5281 caaaaatggt cttcacaggc gacattgagc aaattgactc gccatatctt gactccgggt
     5341 caaacgggct cagctacctc tctgacaaga tgaaggggct tgatctcttc gctcacgtcc
     5401 acctcgtcaa aggcgagaga agcttccttg cagaactggc gagcaagctg ctctgatccg
     5461 gcttttttgc tatttttgca tccggaacag taaccattta gttttgaaga ggaaagtagc
     5521 gtttcataca ctgggatgca agcttaattt tgccgaaact tccacaatgg cacgcacctt
     5581 cccggatgat aagttcgaga gggtatctga tgacagccat gctgacatat atgtgatcaa
     5641 cacatgtacc gtcactgaca ctgctgaccg caaatgcaga caggctgtga ggaagttcgt
     5701 ccaccgcaat cctgacgcct tcattgccgt cgtcgggtgc tatgcccagc tgagacgcga
     5761 ggaggcagcc tccatcgaag gggtcgacct ggtgctcgga acctatgaga aatttgacat
     5821 cgcctcctat atcgacaacc ttcagaaacg aacagctccc gagatccact cctgcgaaac
     5881 cgttgacact gacggattca tgacttcatg gtcagccggc gaccgcacac ggtcattcct
     5941 caaggtgcag gacggatgtg actatcactg ctcatactgc accgttcccc ttgcaagggg
     6001 gaagagcaga aatcctgccg tcagcgaaat agtggccgca gcagaaaaga ttgtcgctgg
     6061 cggagtcatg gagatagtcc tcacaggagt caatgttggt gacttcggga agagcaccgg
     6121 cgagtccctc ggacagctcc tggaaagcct cactaaggtg aatggactgc agagacttag
     6181 actgtcatca attgagccga acctggtcac tgaccggatc attgatatta tctctgaagg
     6241 gaaggtgctg ctgcctcact tccatatgcc tcttcagagc ggaaatgaca gggttctcgg
     6301 acttatgcgc cgccggtacc gcagggaggt gttccgtgac agggtcatga aaatcaggga
     6361 aagaattcca gatgccggcc tgggggcaga tgtcatcgtc ggcttcccgg gcgagacgga
     6421 agaagactat gcagacacat acaccttcct tgagagcctt ccactgtctt atcttcatgt
     6481 ctttgcctat tcaccacggc ccggaacacc tgctgcagag atgccgggag ctgtgaaccg
     6541 ccaggagaag gaaaaaagaa gcagacagct cataaggctg tcggagagcc ggcggatgca
     6601 attcatgcga aacgcctcgg gagaggtaca tgaagttctg tttgaaaaga gaggtcacga
     6661 cggaacagtg gccggactga caggcaatta catacgggtg atgacgccct gggtaaaggg
     6721 actgcccggt tcaatacggc aggtcaggct caccaccctt cgcgacgact cttcaatgaa
     6781 ctgcgaactg acagataaag aagcacaatg aaacgtgccg gttattacat attcgtggcc
     6841 atgagctaca ccatcacact cctccccatg tgggcgctgc acctgctgtc agacctgcta
     6901 tggccgtttt tctatcatgt cgtaaggtac cgtcgcagag tggtggagac aaacctcaga
     6961 aacgcattcc cggaaaagag cccggaggaa cgtaaaaaga tcacaaaaag attctacagg
     7021 catctcaccg acatgatcgt ggagaccctg aaggccatgc acatgtcagc ggagcagatc
     7081 aaaaagcgtt tttccgtgac cgacacctcc ctggccgaca ggttctacag cgaaggacgt
     7141 gatttggttg ccctctgcag ccactataac aactgggagt ggtactcctc aatgcagctc
     7201 tcatcgaaac acagggctgt ggctatttac aaaccattga aaaacaaaga ttttgaccgt
     7261 tttctttata aaataagaac caggttcggg gtatgggtca cacccatgaa ccatatcgta
     7321 agggacctgg taaaatgcag gagtgaaaag attcttacca tgtcaggatt cattgcagac
     7381 cagacacctc cccccgatga caacgcattc tggaccacat tccttaacca ggatacaggc
     7441 ttttacaggg gggcggaaaa agtcgcaata aaatatgaca tgccggtaat cttcattaat
     7501 atagtcaaga gaaaaagagg cttttacgag cttgaataca gcctgatatc agagcacccc
     7561 cgcgaagagg agcccaatgc cattattgcc cgttatgccg ccaggcttga agaggtgatc
     7621 agggcaaaac cggaatactg gctctggtca caccggcggt ggaaacataa aagaccggaa
     7681 aaaaaatgat caggacagcc gtggtcatcc tcaactggaa cggaagaaaa ttcctcgaac
     7741 agtttcttcc gggtgtggtt aaaaacaccc ttgcacccga cacatcggtc attgttgctg
     7801 ataatgggtc tgaagatgat tcgttactgt gggtaacgga aaaccacccg gaggtggagg
     7861 tcatcaggct tgacaacaat tatggatatg caggaggtta caacagggca ctgaaggaag
     7921 ttgaagccga atactacata cttctgaatt ccgacgtaga tgttgagccc gcgtgggcgg
     7981 aaaagctgat caccttcatg gaaatgcacc cgaatgtggg cgcctgccag cccaggatca
     8041 ggtcttactc gaaccgtgac tcttttgagt atgcaggcgc tgcaggaggg tatattgaca
     8101 ggctgggcta ccctttctgc cgtggccgta tctttgacac ggtggagaat gaccacggcc
     8161 agtatgacga tgtccgcgag atcttctggg catcaggctc ctgtatggtc gtcaggggtg
     8221 ccgcattcga ccagtgtgac ggctttgacg agtcgttttt cgtccacatg gaagagattg
     8281 acctctgctg gaggatgcag aacgccgggt ataccatcag ttactttcct cattccgtgg
     8341 tctggcacgt aggaggagga accctcggct acggatcgcc ctccaagata tattataact
     8401 tccgcaacag tcttatcatg ctggccaaga acctcccggc aaagcatctc cgcagaacca
     8461 tcctcacccg acaggtgctc gacggactgg cagctgttat gctgctcttc acggaaggcc
     8521 gcgcagcagc agctgcggtt gtaagggcac acagggattt caggaggcag cggactgcca
     8581 tcagaacctt cagaaccctg acccctaatc ccggaatctt ccatactccg cataccacga
     8641 tgaacaaata tctcctttat gagtattata tacgcaaaag gaaaacattc acagaactca
     8701 agtggacctg aacagaaagc agcagatggc aatattcctt acggttgtac tcaccgtcta
     8761 tatccttacc aacctgtacc tctggtttaa gggccggaga gctctctcag gcgcaggaat
     8821 aaacacggga tggtataatg caatcttcat tgccctggca tcagccttcg tgatcggaaa
     8881 attccttgaa aattctttca ccagtgtttt cactgacata gtaaatgtta tcggcggttt
     8941 ctggatggca ttcatgctct acggattcct tgcgtggctg gcggcggata tcctgatgct
     9001 gttgcagaaa cctttccacc tgatccctcc ggatgtcatc ccgaaactca ggctgtggct
     9061 gtttgcagga ataacctcag taacgctgct gctgatcata atcggattca taaatgcagt
     9121 cagtccagtt accagtagat atgccgttga ggtcagcaag aagcttgaca gcagtcatga
     9181 accgatgcgc atcgttgctg tctctgacat acaccttggc agcatcatcc ggaagagaag
     9241 catgcgccat ctctcggaaa tgataaccgg tgaaaagccc gacctcgtcc tgttcctcgg
     9301 tgatctgctc gacggaagca taggtccggt gctccggggt gatctgctct cgtacctcag
     9361 catacccgca ccacggttcg ggacctatgc cattacaggg aaccatgagt atatgagtga
     9421 cctggttaag tctgtaccat atattgaaag caagggaata agagtgctca aagatgagat
     9481 tatcagcctt gaaaacggcg tccagataat cggccggctt gacagaacgg ccatgcattc
     9541 acagggcatc ggccgcaagc cgctggaaca actgctggct gagactgaca caacggcccc
     9601 ggtgattctg ctcgaccacc agccttatga cctttcagcc ctggcctcaa caccggttga
     9661 catgcaactt tcagggcata cccacaacgg acaaatatgg cccctcaacc ttatcactga
     9721 caggatgttc gaactgagtc acggttacag ggtgttcgga aaggcacacg tgatcgtctc
     9781 ttcaggattt ggcatatggg gtccccggat gcggatagga accaggccgg aaatactctc
     9841 tattaccctg acgggaaaat cagagtaagg gaaaaacctt ttcaagcatt atgcccctcg
     9901 atcctttgac aaggattgtg tagccttcgg gtctctctga cttcagccac tcggcagcag
     9961 cctctgatgt accgaagagg gtggcgggga aatcagctgc cgcctcccgg aaaaggggac
    10021 ccacaagcac tgccctgatg tcaccaatat tcctcagctg cctgataacg gcagcatggc
    10081 cgtgagcact ctcgctgccc agctccagca tatcacccag gataaccatt ttcttatctg
    10141 ccttcagcgc agcaaatgag gctattgcat tctccatgct cgagggattg gcattgtagg
    10201 catcacgtat aacagtgttc ctcccggtct ccgttacctg cgaccggttg ttggagggcc
    10261 tgtaggatga aatggcatca attatttcgt cttccggaac accgaagagc agcccggctg
    10321 ccatcgcagc cctcacattg tcaatattgt atgatccgaa gagcccggtt tcaaaataat
    10381 gttccttgcc gtcaatcacc gcttttacct tcagcagcat caggtcatca cccgcaggcg
    10441 cagcactgag agtgtgccct cccggcgccg agtagggtac gccattggtc atccctgata
    10501 ccagttcaca caggacggga ttatcatcgt tgtatattgc agtcccgtta ctctcccgga
    10561 gcgactggta aagctcagac ttcgctttcc tgatgttttc catggagccg aaaccctcta
    10621 tatgtgccgt tccgatactg gtgatgatac cgtgcgtggg cattgacact ttacagaggg
    10681 ctgctatctc tcccatatgg ttggcgccca tctccaccac aaggaaggct gcatcatcag
    10741 gtgcgctgag aagtgtcagc ggaaccccga tgtgattgtt gaagttgccc ggggtgctgt
    10801 gtacccttcc acgtcgcgac agaacggctg tcagcagttc ctttgtggtt gtcttcccat
    10861 tggttccggt tatcccgatg acaggcatct gcagcctctt ccggtgacag gcagccagtg
    10921 ctgacatggc ctcaagaaca tcgtcaacaa caattattct ttctccgctg agcgagtgat
    10981 catccactat ggaggccagc gctccggcct ccagggcagc tgcggcatgc ctgttcccgt
    11041 catgggcagg acccttcaat gcaaagaaaa tctcaccttt ttttactgcc cgcgaatccg
    11101 tcgttacacc ggcagaattg cggtacagac cgtacaacct ttcaatctcc atctcaatcc
    11161 aaaaagaagc ctcattactt tcgataatga ggctttattc ttacctatat atatacatta
    11221 tagtcctgag gggcttccta ccctgatcat tgcacacctg aaaccaagat catcacgcga
    11281 tgcttcctga tcaagatagc ggcgggttga ggggttgagc cagtaagccc tgtccttcca
    11341 tgatccgccc ttgaataccc ttgccctgtc attgatgagg gtaacatagt cattgtcaac
    11401 ctcgttctgc gaatacatcc tttctgtctg tggcaggtca gcatttcctg accattcaag
    11461 gcttgaagag atacttgaga tgacgtcacc gtcgaggtag ttccggtagt ccgacttctg
    11521 gtaattatac ctgtgggcca catcttccgg cttgacagtg tcaatctgga gtcttcccag
    11581 cctgtccttc tctaccacat cgccattggc gtcacgcctc aggtcggtga atacgttgcc
    11641 cctgaacggg ttgaacatgt caacatcctc tgaggagagc ggacgataaa cgtccattac
    11701 ccactcgtta acgtttcctg ccatgcagta aaggccgtag tcattgggcc agtacgacct
    11761 gacgtcgacc gtgatgctac ctgcgtcgtt caggtttcct gcaacgccca tcatgtcacc
    11821 gcgtccgcgg acgaagttgg ccatgaattc acccctgtac ttgggagctg cattacggac
    11881 gttatgaccg tcccacgggt aaatagccct ttcatatatt ctttcctcga aggtattgcc
    11941 cctgaggccg taagctgcaa attcccactc agcctccgtc ggcaggcggt agcgcggaag
    12001 aaggatgcca tcctcctggc ggacacgcgg atgaagctca tcaactctct gcccggggac
    12061 accttcatac tggcctgcca gataagattc agtattgaag ttgtttgctc ctgactgttc
    12121 cacattggca tcctgccact tgctgtcaag aagaatctgc tcattgaccc tgtcagtacg
    12181 ccagacacag tagtcattgg cctgcagcca gctgacaccc acaaccggat aattattaaa
    12241 cgaggggtga cggaaatagt actgaacata gggttcattc agtgcgagcg gacttctcca
    12301 gaccagagtg tcaggaagag cgcggcggtg aacctccgga taatcgctgt ataccctcct
    12361 cagccagtag agatactcac ggtagtcaag attggtgatc tctgtctcat ccatataaaa
    12421 ggaggaaacg gttaccctgc gcggcgagtt gttccagtca aacattacat cctgatcaac
    12481 ccgtcccatg gtaaaggtac caccttcaat cagcacaagg ttcggaccgg tgacctgttc
    12541 cgaagcctct cctatcgggt aacccaggtc attgacatcg ctcgatttgg gatccctgta
    12601 gctccagcca gtagtcggag acacattatc ctgcttcgct cccttgcagg aaaagaggaa
    12661 gagagcaaca actacaaaaa ccggtaaagc tctgagttta tacatagcaa tataaggttt
    12721 acttgattac tttttaagtt taaaaattgc gttcatttac aaaactctgc accaaaataa
    12781 ctacaaaagg ggcaacttta ttatgtgcgc tcccctactt ttttcaacat ttccgaaact
    12841 aaatattgcc ccggctttta ccatggcggt gcccccgagc gatgtgatgc caccagcaac
    12901 gatgtaactg tatgatatca tgatctttac agatgtcgca tcccatccgg cggaagctcc
    12961 cagagccgta aatccgtttg tgacatgcca tagtgaaaga ctgccggcaa gacccttcca
    13021 tgaggcttcg ctccctaaat aaattttcgt ctcgtcaccc tggatgagga gcgcagctga
    13081 tggcaacagg gagaaatcct ttccgccggg tgtgagcgat attcccccgg tcagtgtcag
    13141 caaccggttc accctgttgt gctcaagggc atcatcgctg agcgatggcc ttgtcaggtg
    13201 catgatcgat gcaccggcga accagtcccc ggacgataca gtaaagcctg tacccaggtc
    13261 gaatctcgta atgggggcag gaggaatata atcggtgcct ccgccggtta tgcccctgaa
    13321 gggatcgatg tctcccggaa ggataaccga tccgcttctt atgccacggg tgattactcc
    13381 ggctgtcagg ccggctgtaa caaagagcct gcgcccggca cggaaatggt atgaatagaa
    13441 tgctccgccc ctgagatcat tcatgatatc ccccagcata tcatcagaga cccatgcacc
    13501 ggcacctccc cgcagggaac tgaaataccc gtcatatgat gcgtatacag acctcagtcc
    13561 gaagccgttt cccggaagaa aggaaaatgc agagatcttt accgtggagg tgctctcaac
    13621 cccggcaaat gccggattat gcatgacttg catgccagat accgacccgt accaggtgtc
    13681 ctgcccgctg agggtcaacg ggcagatgag caatgccgcc agaagagtcc tgtatctgag
    13741 cttcatggtt gtgtttcaga attatcaacg ctgccgggcg gtcattattt tgtccccggc
    13801 tgcatcaata cgggagcaaa gataggtgac ttgactatct ttgcgttgat tatgatgaaa
    13861 agtgttgcca ccggcctcct ggttatggcg tccgcgctta acgcagcgtc acaggaatat
    13921 tcggcttcgt ctgtgccggc agccggtgca tcaggccggg cttcggtatc ggtgcagggc
    13981 gcagctgagc aggaatattc ggcttcgtcc gtgcttggaa acgggcagtg ggtgaagatt
    14041 gccgtgaccg aagagggcat ttaccggctc gactattccc gtctgaaaga gatgggattc
    14101 accgatccgt cttcagcggt cctttacggc aacaatgcag gccagctctc attcatgaac
    14161 gatgacactg ccccggatga cctgaaaaaa attgccttca gagcagaaaa gggaaccgac
    14221 ggggttttca acgaaggtga ttatcttctc ttttatgctg aaggcactca ccgttggatt
    14281 gctgatcccg gcaggcgcgg cagccggttc gtgaggcatc attactcaga tacggcatgg
    14341 tattttataa cctcgcaacc cggaggggcc ctgacagtgc agaccgaaac acccccggca
    14401 gcatcaccct caatcatctc atcatccacc gacctgtaca tgagacacga acgggaggag
    14461 atcaacctga taaggtcagg aagggagtgg taccagcagg tggttcccgg tactcagaac
    14521 aacatcggac cggggtttac agacctccct gccggcggga aaatacacta caccataaga
    14581 gttgccgcaa ggtctgacac cgagacctcc ttcatcctca gacagggagg agaaacgatt
    14641 aaaaccatca cagtcccggc cgtgaccatg accgatatca acggcgttta tgcagccacc
    14701 atgactgttt cagattccat cgtctcctcc tccggatcac ctgcattcag cctcgcattc
    14761 tcctcaggcg gtaatatggc cgccaccggc tacatcgact atgccgattt cacggcaagg
    14821 tcctcactgg tctggcgcga caggaacctg ttcatcagcg acagcaggtc tgtgggcacg
    14881 tcggctgtca cccggttcac cgtggaggga tcatcttcgc tgagcatctg ggacatctcc
    14941 aatcccgctg ccccgctgac catacagaca acaccatcct ccgggaacac agtctttgtg
    15001 gcggcaactg acagcctcag aagattcgtt gccttcagcc tggcacaggt aaaacagcct
    15061 ttaagaagcg tcagggtgcc cgcccagaac ctgcacgcca tgatgcctgc cggtatgatc
    15121 atcgtcactt acccgctatt tatgccgtat gccaaaaaac ttgcggaatt gcatcttgcg
    15181 gatgacggtc tgacatcatt gatcgtaacc ccggaacagg tctacaatga gttttcagga
    15241 ggggtgcccg atgcgactgc actaagaaac ttcatcagga tgatccggga gagggggatg
    15301 cagtcgggtc agcaaacgag atacctgctg ctctttggtg acggctcgta tgagaataaa
    15361 accccgccgc ccggaaacag ctcattcatc cccacatggc agtctgtcaa ctcgacaacc
    15421 ggtgtactct ccttcacttc agacgatttc tacggcctgc tcgatgatgg cgagggtgag
    15481 gctgacggat tccttgacat gggcatcggc cggctgccgg cgtatgatac cgcatctgcc
    15541 ggcattatgg taagaaaaat ctcatcctac cttcaggggt caaatcaggg ctcatggcgg
    15601 aatattctgt gccttgtcgc agacgatgag gattctaatc tgcatatgtt tgatgctgaa
    15661 ggccttgccg aagcagccac caccgcggca cctcccctga cggtggagaa aatatacctc
    15721 gatgcttacc gccagatgac ctctgtctcc ggtgattcct accctgacgc ttcgagggcg
    15781 gttgacgaca ggatggcggc cggatgcctg ataatgaact acgtgggtca tggcaacgaa
    15841 tcaggcctcg cccatgaaag agttatcagg accggcaata tcaactcgtg gaagaacagc
    15901 aggatgctgc cgctgttcat caccgcaaca tgtgagttct ccaggtttga tgatgtggag
    15961 ataaacaggg cttcctcctc gataaccgca agaaactcag ccggcgagat ggtgcttctc
    16021 aatcctgacg gcggagggat tgccctgatg agcaccacca gggtagtgta ctcagctcct
    16081 aactacacac tgaaccgggc catctatgat tatgctttca ggacagacgc tgaaggccgg
    16141 tcgatgaggc ttggcgacat catccggcag gccaaggtca gctccggaac ggggatgaac
    16201 aaacggaatt tcctgctgct gggtgaccct gccctcaggc tggcatggcc ttcgacaggg
    16261 aaagtggtga ctgacagtat caacgggatc catgtctcga tgccttttga cactctcaag
    16321 gccctttcac tgatgactgt cagcggtcac ctggaagatg ccgcaggaaa cctgatgacc
    16381 ggattcaacg gcaccgtgga acctgctgta tttgacaagc ccggacacgt cagcaccctg
    16441 gccaatgacg gcggttcccc gatcaccttc ccggtggatg gcaacgtgct cttccggggt
    16501 aaaacaaatg tcaccggcgg ccgcttcagc ttttcattca tcgtacccct cgacatcaac
    16561 tacagctatg gcagcggatc cgtgaaatac tatgcataca acggcgccac cgacctcaac
    16621 ggaagcttca cgggaatttc ggtgggaggt ttctcggatg cggcatctga tgacactgaa
    16681 ggaccacaga taagactctt catgaatgac acactgttca atgacggcgg agtgaccgat
    16741 gcatctccgg tactgctagc ccttctcagc gacaggtcag gcataaatgc caccgggacg
    16801 ggaatcggtc atgacataat agcctggctt gacgacgaca tgtcagaagc tgtggttctt
    16861 aacagccttt tcagggctga cgttggcagg catatctcag gctctcttgc ctatcctctg
    16921 cttgtgaccg gccaggggaa acacacggtt tcactcaggg catgggataa tctcaacaat
    16981 ccgacagtgg ccacgctcag gtttgctgtg gagaccggcg gcaccttcag gctcacggac
    17041 ctcctcgcat tcccgaatcc ggtgacagac ggcacaaagt tcactgccgg tcataacagg
    17101 ccgggtacgg agattgatct gaccataaca attttcagtt ctgacgggcg tgccatgaaa
    17161 gtcctgagaa ataaaagttc cggcgccgga tatgcccttc ccgacatccc atgggacggc
    17221 tgtgacgaga acggcggaag ggtggcaaga ggactctatc tgtggcgtgc agaagcagtt
    17281 accgcagatg gcgagaaaac atcggccaca ggccgtttca tcattttgta aaacaacaca
    17341 ataagtagcc cttatttttg ttttatattt gcacaaattt cgaacaagga caaatatgaa
    17401 aaacaaggcg ctgactgtaa gcattatact cctcttttca atttttgttt caccggcatg
    17461 ggcacaggat gatgatccgt ataacgttat tcagactgct gtcccgttcc tgaccattgc
    17521 tcccgactcg cgcgccggag cccttggcga tgcaggcgta gccacttcac cggacctttt
    17581 cagcatgcac tggaacccgg caaaatatgc cttcatcgac ggtaacggcg gattcggaat
    17641 gtcttactcg ccatggctga gggctcttgt gcccgacatc aacctggctt acctttcggg
    17701 ttattacagg gtagacagca ggcaggtact ggctgcaagc ctcatgtact cctcgctcgg
    17761 cgagatccag tttaccgatg actacggaaa ctaccagcgt aattacaatc ccaatgaatt
    17821 ttcagttgac gtggcttact cccgcctctt ctccgaacac tggtcgggcg gcgttgccat
    17881 gagattcatc tattcaaaca taaccggagg aatgactgtc ggaacaaccg aaacaaaagc
    17941 aggtattgct tttgctgctg acgcttcggc atactatacc aacaaactta aactgggatc
    18001 acgtgacgga aacattgcgg caggaataaa cctgtcgaat ataggctcaa agatgagcta
    18061 tacaactgat gttgacccgg acttcatccc tatgaacatg cgactgggaa caactttcag
    18121 cattaacctc gacaggtaca atgcaatcgg attctccctt gacgtcaata agcttctggt
    18181 gccaacccct ccagtatatg acgaggaaag caatattatc gacggtaagg acccgaacgt
    18241 atctgttccc gtggcaatct tccagtcctt ctatgatgcc cccggcggct tcagcgaaga
    18301 gatgaaggag ttttcccagt catttggcat cgagtactgg tacaaccagc agtttgcact
    18361 gcggacagga tatttcaatg aggcccagag caagggaaac aagaaatatt tcaccgtagg
    18421 agccggattc aggctgagca tcttctcact tgacttctcc taccttgttc cgattaccca
    18481 gaacaacccg ctggcacgta ccctgagatt ctcactggga ttcgagttcg actccctacg
    18541 caatctcagc cagaagtaaa tcacaaaatg acactccacc agaggatagg acagggcatc
    18601 gactttcatc gccttgagga gggtcgtgaa ctctggctcg gaggcgtgcg catcaactct
    18661 gcaaagggat gtgttgctca ctctgacgga gatgtcctta tccatgccat ctgcgatgca
    18721 ctgctgggtg cggcaggact caatgacatc ggctatcact tccctgacac atcggaggag
    18781 ttcaggggga tcgacagcaa gatcctgctg aagcgggtgg ttgagctgat caccggtgca
    18841 ggttacagga tagtgaacat tgacagcact gtctgcctcg agctgccaaa gctggcgcca
    18901 tatatcaccg ccatgcgtac gaccatggcc gccattgcga acatctcacc cgatgctgtc
    18961 tcggtcaagg ccaccaccac agagaagatg ggtttcaccg gccgcggaga ggggatagtg
    19021 gccctggcca tatgtctgct ggcagacctg atcccggata aggtctgaag gtctgaaggt
    19081 ctgaaggtct gaaggtctga aggtctgaag gtctgaaggt ccttttcact attgccgatt
    19141 ttcgattttt taattcggaa atcgcaaatc gc
//
LOCUS       DAOPXU010000050        18965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_3040_length_18965_cov_14.795611, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000050 DAOPXU010000000
VERSION     DAOPXU010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18965
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3040_length_18965_cov_14.795611"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..1132
                     /gene="nadA"
                     /locus_tag="PKK03_06665"
     CDS             185..1132
                     /gene="nadA"
                     /locus_tag="PKK03_06665"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNX84120.1"
                     /translation="MVVDNNRIKEIEALKKEKNAVILAHYYQTSDIQDIADFIGDSLA
                     LSQKAAETKADIIVFAGVKFMAETAKILSPEKKVIIPDLMAGCSLADSCKKEDFEKFI
                     SDNPGRTVITYVNTTADIKALSDIACTSSNAKQIIESLPPDEKIIFGPDRNLGNYIKS
                     LTGRDILVWDGACHVHEQFSLEAILKLKNENPGSKIIAHPECEKPVRLVADYIGSTSG
                     LLSFISKDPGKIFIVATEPGILYQMKKAEPEKILIPAPPKDSTCGCSECNFMKLITLE
                     KIYKSLKFEEPEVTVDPSIIERAAVPIKRMLEMSAKLGL"
     gene            1134..2684
                     /locus_tag="PKK03_06670"
     CDS             1134..2684
                     /locus_tag="PKK03_06670"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HNX84121.1"
                     /translation="MKRIYLIMLVFIPALLTAQQNVQLQDGYQQFRYPNGNISSEGYI
                     RNGKPDGYWKSYYVTGVLKSEGRRTSFLLDSLWVFYDQAGDTTEKISYLLGKRNGYSL
                     KYKKDPVHGIYVFSSELYAGDRKEGLAKIFFPDGKLKQTIPYSDSKKDGLSREYDHDG
                     KIITLLEYNNDFLISRERINFTDASGMKQGEWLDFYPDGSRHIERNYRDDQLHGYYKE
                     YDERGRLLVTLLYDNGKVTGTDIDNSAEIDIQNRYDDSGRLIYSGPFKEGIPVGIHRE
                     YNTDGTIKSARIYNDIGVLISEGIVDAEGNRTGPWKDFSPGGVLIAEGNYTANRRTGT
                     WKFYNSSGRLEQAGSYSSGRIDSTWRWYYPEGELLREEDYYQGKRDGSYTEYTRTGEI
                     IARGTYADGERNGLWKVTTGDNTEEGEYLLGLRNGEWKSFYPDGKLRFKGSYRQGNPE
                     GHHTVYFENGRTKEDRYYRNGFRTKTWKKYDELGEVILTITYRDDVETSINGVPVNLP
                     ESSVKRLK"
     gene            2692..3711
                     /gene="ruvB"
                     /locus_tag="PKK03_06675"
     CDS             2692..3711
                     /gene="ruvB"
                     /locus_tag="PKK03_06675"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNX84122.1"
                     /translation="MEEQFNLRKEITLAGDAELEKQLRPLTFSDFRGQQRITDNLSVF
                     VTAALQRGEALDHVLLHGPPGLGKTTLASIISNELKVKLKVTSGPVLDKPGDLAGLLT
                     SLDEGDVLFIDEIHRLHPVVEEYLYSAMEDYQIDIMIDKGPGARSVQLKLNPFTLIGA
                     TTRSGLLTSPLRARFGIKFHLEYYDAPVLKGIVKRSASIMNVAINESAAAEIASRSRG
                     TPRIANALLRRVRDFAQVKGSGAIDLDITRYSLEALNIDKHGLDEMDNRILKTIIEKF
                     RGGPVGLNNVATAVGEESGTIEEVYEPFLIKEGYIKRTPRGREATERAYRHLGIVRGT
                     EETLF"
     gene            complement(3782..4483)
                     /locus_tag="PKK03_06680"
     CDS             complement(3782..4483)
                     /locus_tag="PKK03_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNX84123.1"
                     /translation="MKQHDFTISFCDKCAFETSSVFRFLTRDETDRLNFEKDFRRYKR
                     GDILYHEGNRISGFYCINSGIIKVFKTGIDGKEQIIRFARKGEIIAYRSVLSNEPACT
                     SAEVIEDCQVCFIPSELLIQFVKTNPSFAFEVVKLTCHELAEANSYITDIAQKTVRER
                     VAEVLLSLVKDFGLDEQKYLRISLTREELANIVGTATESVIRLLSEFKSDRLIELNGR
                     KIKIIDQKGLEKISS"
     gene            complement(4529..5572)
                     /gene="pheS"
                     /locus_tag="PKK03_06685"
     CDS             complement(4529..5572)
                     /gene="pheS"
                     /locus_tag="PKK03_06685"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNX84124.1"
                     /translation="MKERIAELKKEIEEYNIDGEGSLEQFRIAYLSKKGLISVLFEGF
                     RNVPPEAKRETGQLLNDLKQSATDKYNEHKERLAMTEVAGPSVDLTMPAYPFGEGTRH
                     PISIVRNEIIDIFSRIGFTVSEGPEIEDDNHVFTMLNFASDHPARDMQDTFFIEKKSG
                     TESDPHDVLLRTHTSSVQVRVMENQQPPIRTISPGRVFRNEAVSARAHCIFHQVEGLY
                     VAENVSFADLKQTLLYFATELFGKDVSIRLRPSFFPFTEPSAEMDISCNICGGKGCNV
                     CKGTGWLELLGCGMVDPNVLEACGIDSEKYTGYAFGMGIERATMLKYGVNDLRLYFEN
                     DIRFLNQFRTEIL"
     gene            complement(5587..6384)
                     /locus_tag="PKK03_06690"
     CDS             complement(5587..6384)
                     /locus_tag="PKK03_06690"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX84125.1"
                     /translation="MRTSFRIVMTLAAAIIIAGCSAGSKKGVRAVLQTTEGDIVISLS
                     DLTPLHRDNFIKLAESGFYNGVTFHRVIKNFMIQTGDGSTRQDSTLISDDYTVPAEIN
                     DSLFHRRGVVAAARTGDDVNPERNSSGTQFYIVQGKVYNDEELAATIERIEYNRRQFL
                     YNKALLDLRREAEQAGKEMTEDEIQQSAIIKAYDAMEKAGTYVMPEHHRNAYKTVGGT
                     PFLDGAYTVFGEVLEGMEVVDAIAGTATDLTDRPVQDIRIVKVKIIK"
     gene            6437..7909
                     /locus_tag="PKK03_06695"
     CDS             6437..7909
                     /locus_tag="PKK03_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNX84126.1"
                     /translation="MNDIRKLAGQTVIYGFGTVVPRFLNYALLTPFYTRIFGLEQYGV
                     VTELYAWMVLALVILTYGMETTYFRFAGKKAPAEKVYGTALISLFSTSALFLLAVSLC
                     INQVSGFLGYEQHPEYIRMFAWIVAIDAFSAIPFAWLRNNNRPVVFSALKIINVVVTI
                     VIAFFFLKIAPSLAEKGSTWIYKIWNPDFQVGYVFVANLAGSAITLICLIPFIFRMQP
                     VFDRKLLGRMLAYSWPLLIGGMAGSLNEVLDKILLRRLIGGTEGLATVGMYGAGYKVG
                     VLMSLFIQMYRFAAEPFFFEKAGSRDAKETYAVTMKYFVITALILFLGINLYPEAVRI
                     LIGDIFRESLIVVPIVSMGYLLLGIFINQSIWYKVDDKTIYGAWITLVGVAVTVAVNV
                     IFVPVFGYIAAAWAHIACYLSMVIVSYFVGQKYYPIKYETGKIFMYIVLAVALLFVSM
                     LLAGENKILNTVIDTLLLFIFFAVAEIRDKFFTLLFKRGS"
     gene            7906..8340
                     /gene="dut"
                     /locus_tag="PKK03_06700"
     CDS             7906..8340
                     /gene="dut"
                     /locus_tag="PKK03_06700"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNX84127.1"
                     /translation="MKIRIVNKSSHPLPAYETEHSAGMDLRAFTGGPVTLKPMERKLI
                     PTGLYIELPVGYEAQVRPRSGLAIRHGITVLNSPGTIDADYRGEIGVILINLSESEFT
                     INDGDRIAQMIISQYVKVELTGTDSIEMTARGEGGFGHSGRI"
     gene            8337..10052
                     /locus_tag="PKK03_06705"
     CDS             8337..10052
                     /locus_tag="PKK03_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84128.1"
                     /translation="MKNTVILVLLLLAAAGCSEKAVSPVLTRDREARADNRYDYMLAE
                     ALRQKYVGELSEAAVLFEKCIELDRNRAVPYFELAQMYSAAGMSGKSLQYASTAARLE
                     PGNYWYQLACGSLFTQYEMKDSALVYFSRALKADSKAVEVNGILAGLYSEKGETSKAD
                     SLFKVLDSEGALTPDMSLMMISGLLGMNELDEASKRTVKLLEQHPEETRFRALLADIY
                     YEQGLKEKSDSIYTEIIEKDPDDIETQLLYLISLVYRKEYSGISGFLGNIFRSDVVER
                     ERKIAVARRLAGDSAYIKDNAESLLGSLLILEEQYPDDEEIISLRPGVYETTGMIDLA
                     IQRYEEIIKSVSQGFWFRERLMILYAGKKEYGKLYELAAGYSRDNNRSLLGKIYYALA
                     AMELKEYAVADNELKKALILAGNNDELKIQVLSLEGDLKYRMKDYDASYASYEEALKL
                     SPDDPLLLNNYAYFLAEGNRDLKKALKMAMKVMETGSDNPTYIDTYAWVLYKMGKYRD
                     AHREMLRIFELSSERDPELLEHMGFVKKALGKCEEAVIYWMEALEKDNTKIYLEEEIK
                     KCMKQ"
     gene            10037..10801
                     /locus_tag="PKK03_06710"
     CDS             10037..10801
                     /locus_tag="PKK03_06710"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HNX84129.1"
                     /translation="MHEAIKYVAIISFLFLLAGCSALKKEKTTGPDVQPADAGYSAII
                     GNVASNNISERGFEIRKGSIELEGTEVEGKFGLHARLNSKGDFYGSVRGPLGIELVRV
                     LMVGNDIAAIDRFNRKIYIGKKDQVLKKNGMPSDFMSIIFGDMPSEDEVELIRSEKNE
                     IVVASGNGDFSSVISICLDYMKVCSRKINANTSDHEIYMLFGEFGESGGMKYPSEISM
                     EEKKKMFHVKLSIDDLIYGYDSDIELNLPSYKRESF"
     gene            10798..11979
                     /locus_tag="PKK03_06715"
     CDS             10798..11979
                     /locus_tag="PKK03_06715"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNX84130.1"
                     /translation="MKYLFLILFLAVWSASVLSGQSRSELEEMRKKNLQEIEYVDRLL
                     KTTASQKTENVNELRVIGRKLNLREKLIDEYGQEINLLEYRIGLNALATEMLEQDLNE
                     LKKDYANSILSAYKATKGTPALAFILSAADFNQGYKRLKYIQQVAKYRRKESETIETL
                     YTQLQGTKERLEKDRKNVSDLKNKEVRQKQILRQEQDRKEKLVSNLSRKEKQLKQELD
                     EKRRIARQIEKEIERLIEAERKKSETVPMSAEMKVIGESFGENRGRIPWPVDRGIITS
                     HFGSHQHPVLKNVIEDNIGIEITSSGPTKAKSVFKGEVGGVFAISGANMAVIIRHGKY
                     LTVYQNLVNVVVKAGDSVSLGQVIGDVYVDNGDGGKAELKFMVYEEKKKLDPEQWLTK
                     K"
     gene            12039..12431
                     /locus_tag="PKK03_06720"
     CDS             12039..12431
                     /locus_tag="PKK03_06720"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX84131.1"
                     /translation="MESITINSDIEKLRVVETLVDTLSKKLGISDEVYGKILISTVEA
                     VNNAILHGNKGIEDRTVTVDFTADGNMFEVTVTDQGEGFDYNHLPDPTDPENIENLHG
                     RGVFIMRSLADIIEYNLPGNQVKMRFKY"
     gene            12436..12858
                     /gene="ybeY"
                     /locus_tag="PKK03_06725"
     CDS             12436..12858
                     /gene="ybeY"
                     /locus_tag="PKK03_06725"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNX84132.1"
                     /translation="MKVSFHYDIEKFRLRDSRIIKKIINRIVTDARLRPGIIDVIVTG
                     DEKLYEINREFLGHNYYTDIITFNYNEAGTVNGEIYISAERVRENSVKFNVTFGSEMR
                     RVIFHGLLHLCGYDDRTEGEKNKISEMEEMYLALSYAE"
     gene            12855..14729
                     /gene="mnmG"
                     /locus_tag="PKK03_06730"
     CDS             12855..14729
                     /gene="mnmG"
                     /locus_tag="PKK03_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNX84133.1"
                     /translation="MIFSYDIIVVGGGHAGCEAAHIAAVMGAKTLLVTMDMTKLAQMS
                     CNPAMGGIAKGQIVREIDALGGSSGIITDRSMIQFRMLNRSKGPAMWSPRAQCDRMLF
                     SREWRKLLEQTENLAIWQEEASGIIIEDGVVKGVTTTFGTELRADAVILTNGTFLNGL
                     MHVGFKTMSGGRSGDPASRGLSEQLRELGFEVERLKTGTSARVDGRSVDFSAMTEQKG
                     DDDGRCFSYLHDRVDIKDQRSCYITHTNEKAHGILREGLKFSPLYTGRIKGRGPRYCP
                     SVEDKIVTFADKEAHQLFLEPEGWETNEYYINGFASSLKLETQISALREIKGLENVII
                     YRPGYAIEYDFFQPTQLKHSLETKRVGGLFFAGQINGTTGYEEAAAQGLVAGINAVQK
                     VRGKDPFILKRDEAYIGVLIDDLVTKGIDEPYRMFTSRAEYRILLRQDNADERLTPMA
                     VELGTAEPGRIERLVEKYRMVDELMVFLKNCSISPEEINGFLKEKDTAEINQKIKAIN
                     IATRPQVSLEELLEQIGGSGKLSYAGSVRKTEIIEAAEIKIKYSGYLERERGIAEKIM
                     RLEGLKIPEEINYEELASISTEGRQKLSKIKPDTIGQASRISGVSSSDVSILIMYLGR
                     "
     gene            14770..16416
                     /gene="ade"
                     /locus_tag="PKK03_06735"
     CDS             14770..16416
                     /gene="ade"
                     /locus_tag="PKK03_06735"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HNX84134.1"
                     /translation="MNIAGKIIDVHKKRIYPGIVTIKAGKIESVTETERSPDHFIMPG
                     LADAHVHIESSMLVPSHFAVAAVRHGTVAVVTDPHEIANVLGKEGVKFMLDNAENVPV
                     KIVFGAPSCVPATSLESAGAKIGSADIRELIEEGKVKFLAEMMNFPGVIYDDAEVHHK
                     LEAALNAGIPIDGHAPGLTGEELKKYISAGITTDHECSSLEEALEKISGGMKILIREG
                     SAARNLEVLAPLISYYPEKVMLCTDDLHPETLLRGHINKLVARLISMDYNLFDVVRAA
                     SVNTYEHYRISAGTLRPGDPADFIIVDDPKTMNVLQTWIDGKCVFDGDKPLFSPGEIV
                     KINKFKCTRLLPDEIRVINEGGKINLIVAVNGELTTDADIVQAGEGEFVAARPEEDIL
                     KIVVKERYNDRPPAVAFIRGFGMKHGAMASSVAHDSHNIIAVGTNDRDIVNAINLIVD
                     AGGGMAVTSEDGAEILKLPVAGIMADLPVTAMAARYEKLSETVRSLGCRLDAPFMTLS
                     FMALLVIPKIKMSDRGLFDGLTFKHIPLFVPDPYSKYKTY"
     gene            16609..18402
                     /gene="uvrC"
                     /locus_tag="PKK03_06740"
     CDS             16609..18402
                     /gene="uvrC"
                     /locus_tag="PKK03_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNX84135.1"
                     /translation="MSAVETLKSTVSALPDKPGVYQFIDASGTILYVGKARSLRKRVG
                     SYFTKNQSGKTRVMLSRAADLRHIVVDNESEALLLENSLIKKHQPRYNILLKDDKTYP
                     WICVKNEHFPRVFMTRKLIADGSAYFGPYTSVPAVKTLLELIRHLFQIRTCSLPLNSR
                     SISEGKFKVCLEYHMGNCKAPCIGFISEEEYNLQADRIKEIIRGDLSGVTAYLESQMR
                     QYAGDLKFEEAQKIKEKLELLSRYRSKSVVVNTSVKNVDVFGCSAGEGSMYISYMKVV
                     TGAIVQSWSVELKMRMEEEKESILSTAVTEIRQRVSSDSPEVIVPFMPDIQLDKVKYS
                     VPQRGDRYKLLDLANRNAVFFRLERQKKTADRSKENRTEKNLEKMKKDLHMPALPLHI
                     ECFDNSNIQGESAVAACVVFRNGRPSNREYRRFNIKTVTGPDDFASMEEVIFRRYRRM
                     LDEQTPLPQLVVIDGGKGQLSSAVKSLEMLGIRGRLTVIGIAKKLEEIYFPGDSVPLY
                     LDKNSITLKIIQHLRNEAHRFGINFHREKRSSVMNRSILDEIPGIGEKTKERLLKKFG
                     SVRNLSGATAADLEKVVGAKKASLLAAALKN"
     gene            complement(18515..>18965)
                     /locus_tag="PKK03_06745"
     CDS             complement(18515..>18965)
                     /locus_tag="PKK03_06745"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNX84136.1"
                     /translation="EKEMKDVNRFLSLGTYAIAGVSRDPKKFGQAMFRDLRKKGMDVV
                     PVNPAAETIDGVKCYRSVSELPAGIRGLIFMTPKEETLAVAKEAIAHGIKDLWIQQGA
                     ESKETIAAMENEDVNLIHHQCIMMFWKPDGIHSFHRFLKKIFGGLPA"
BASE COUNT         5376 a         4151 c         4898 g         4540 t
ORIGIN      
        1 caattgttaa ttgtatttct aattgtctct ttttcagaaa aggtacgttt tttgatgtgt
       61 tgaaaagatg ttattattga tttaactccc atttttgcaa atgaaatcgt aaaatagttt
      121 taacagaact aacaggatat tataaccatc atatagataa aatttaaaaa taataattaa
      181 taaaatggtt gttgataaca acagaataaa ggaaatagaa gctctcaaaa aagaaaaaaa
      241 tgcagtaata ctggctcact attatcagac ctccgacatc caggacattg ctgactttat
      301 aggcgacagt cttgccctgt cacagaaggc cgctgaaacc aaagctgaca taattgtttt
      361 tgcgggagtt aaattcatgg ccgaaactgc aaagatacta tctcctgaga aaaaagtgat
      421 aatcccggat cttatggccg gatgctctct ggctgattca tgcaaaaagg aggattttga
      481 aaagttcata agtgataatc cgggcagaac ggttatcaca tacgtgaaca caactgctga
      541 tatcaaggcc ctgagtgaca ttgcatgcac atcatcgaat gcaaagcaga taattgagtc
      601 acttcctccg gacgaaaaaa taatttttgg tcctgacaga aatctgggca attatataaa
      661 aagccttaca ggacgcgaca tacttgtctg ggacggagca tgccacgtgc atgaacagtt
      721 ttcacttgag gctattctta agctgaaaaa tgaaaacccg ggatcaaaga ttatcgctca
      781 tccggaatgt gaaaagccgg tcaggctggt ggctgattac atcggctcaa cttcaggact
      841 gctttcattt atcagcaagg atccgggaaa gatattcatt gttgccactg aacccggaat
      901 tctttaccag atgaaaaagg cggagccgga aaagatatta atccccgcac cacccaaaga
      961 ttcaacctgc ggatgcagtg aatgtaattt catgaagctc atcacacttg aaaagatata
     1021 taaatccctt aaatttgaag agccggaggt gactgttgat ccatcaataa ttgaacgggc
     1081 ggcagtgcca attaagagaa tgcttgagat gtcggctaaa ctggggctgt aaaatgaaga
     1141 ggatatatct gatcatgctg gtttttatcc ctgcgctgct gactgcgcag cagaacgtgc
     1201 aactgcagga cggatatcag cagttcaggt atccgaacgg aaacatctcc agtgaaggat
     1261 atataagaaa cggaaaacca gacggatact ggaaaagtta ctatgtcacg ggagtactca
     1321 agtcagaagg caggcgaacc agttttctcc ttgacagctt gtgggttttt tatgaccagg
     1381 caggtgacac cacagaaaag ataagctacc tccttggcaa aaggaacggc tattccctga
     1441 aatataagaa agatccggtt catggaatct atgtcttctc ctccgagctt tatgccggtg
     1501 acaggaagga aggcctcgca aaaatatttt ttcctgacgg gaagctcaaa caaaccatac
     1561 cctattctga cagcaagaag gacgggcttt cgcgcgaata tgaccatgat ggcaaaatca
     1621 ttactcttct ggaatacaac aatgatttcc tcatcagccg cgaaagaata aacttcaccg
     1681 acgcatcagg aatgaaacag ggagagtggc tcgactttta tcccgacgga agccggcata
     1741 ttgagcgaaa ctaccgtgac gaccagctgc acggttatta caaggagtat gacgagaggg
     1801 gacgcctgct ggtcacactg ctgtatgaca acggcaaagt gaccggaaca gacattgaca
     1861 acagcgctga gatagatatt cagaacaggt acgatgattc aggaaggctg atctattcgg
     1921 gtccctttaa agagggcatt cctgtcggga tacaccggga gtataatact gacggaacaa
     1981 taaagagcgc acggatatac aatgacatcg gggtgctgat atcagagggc atcgttgacg
     2041 ccgaaggcaa ccgtaccgga ccatggaagg atttttctcc cggaggagtt ctgattgcag
     2101 agggcaatta tactgccaac aggagaacgg gaacctggaa attttacaac agttccggca
     2161 ggcttgagca ggccggatca tacagcagcg ggcgtattga cagcacctgg cggtggtatt
     2221 accctgaagg agaactgctt cgcgaggaag attactacca gggaaagagg gacggcagct
     2281 atacagagta tacccgcacg ggtgagataa ttgcccgtgg tacctatgct gacggcgaaa
     2341 ggaacggatt atggaaagtc accacaggcg ataataccga ggagggagaa tacctcctgg
     2401 gcctccgcaa cggtgaatgg aaatcatttt accctgacgg gaagctcagg tttaaaggaa
     2461 gctacaggca gggaaaccct gagggccatc acacagtgta ctttgaaaac ggcaggacaa
     2521 aagaggacag atactacagg aacggcttcc gcaccaaaac atggaagaag tatgatgaac
     2581 tgggcgaggt gatccttaca atcacctacc gtgatgatgt tgagaccagc atcaacggtg
     2641 tgcccgtaaa cctgcctgaa agcagcgtaa aacgtttaaa gtaacgggca gatggaggag
     2701 cagttcaacc tgagaaaaga gatcactctt gccggtgatg cggagctcga aaagcaactt
     2761 cgcccgttga ctttttctga cttcaggggg caacagcgca ttaccgacaa cctgagcgtt
     2821 tttgtcacag ctgcgctgca gcggggcgaa gctcttgatc acgtgcttct gcatggtccg
     2881 cccggactgg gaaaaaccac cctcgcttca ataatatcaa acgagctcaa ggtaaagctc
     2941 aaggtcacat cgggtccggt ccttgacaag cccggcgacc tggcgggact tcttaccagc
     3001 ctcgatgagg gtgatgtgct tttcattgac gagatacacc ggctgcaccc ggtggttgag
     3061 gagtatctgt attccgcaat ggaggactac cagatagaca taatgattga caaggggccg
     3121 ggagcacggt cggtccagct gaagctcaat ccgtttaccc tgataggcgc cacaacccgc
     3181 tccggactgc ttaccagccc tcttcgggcc cgcttcggaa tcaagttcca ccttgagtac
     3241 tatgatgccc ccgttctcaa gggaatagtc aaaagatcag cctcgattat gaacgtggcc
     3301 ataaatgaaa gtgctgctgc tgaaattgca tcgcgcagca ggggtacgcc aagaatagcg
     3361 aacgctctgc ttagaagggt aagggatttt gcccaggtaa aaggcagcgg cgccattgat
     3421 cttgacataa ccaggtactc gcttgaagcc ctgaatattg acaagcacgg ccttgacgag
     3481 atggataacc gcatactgaa gaccattatt gaaaagttca ggggagggcc cgttgggctg
     3541 aataatgtgg caacggcagt gggagaggag agcggcacca ttgaagaggt ttacgaaccg
     3601 tttcttatta aggagggtta catcaaacgt acacctcgcg gccgcgaggc tacagaacgg
     3661 gcatacaggc atctcgggat agtgagggga accgaagaaa cgctattctg atcaggcggc
     3721 agcgcccgcc acggaacaaa cccggcggcg ggagcaataa acccgcggtt cagcttcagc
     3781 ctcaggaact tatcttctca agacctttct ggtctattat cttgatcttg cgcccgttaa
     3841 gctcaatcag acggtcgctc ttgaattccg acagaagacg tataacagat tctgtggctg
     3901 ttcccacgat gtttgcaagc tcttcgcgcg tcagcgagat acggagatat ttctgctcgt
     3961 caagaccgaa gtccttcaca agactgagca atacctcagc aaccctctcc cttactgtct
     4021 tctgggcaat atcagtaatg tatgagttgg cttcggcaag ctcatgacat gtcagtttca
     4081 ccacctcaaa agcaaaagac gggtttgtct tcacaaactg aatgagcagc tcagatggga
     4141 tgaagcatac ctggcagtct tcaataactt ctgcagaggt acatgccggc tcgttgctca
     4201 gcacggacct gtaggcgatt atctctcctt tcctggcgaa tctgattatc tgttccttgc
     4261 cgtctatgcc ggttttgaag accttgatga ttcctgagtt aatgcaataa aatccgctga
     4321 tccggttacc ctcatgataa aggatatcgc ctctcttgta acgcctgaaa tccttctcga
     4381 aattcagcct gtctgtctca tcccttgtaa ggaagcgaaa aaccgaactg gtttcaaaag
     4441 cacacttgtc acagaatgat atggtaaagt catgctgttt cataaatctg gacattaggt
     4501 aacaaaggtg ggcattaatt ccctaatatc ataaaatttc cgtccggaac tggttcagaa
     4561 accttatgtc attttcgaaa taaagtctca ggtcattaac cccgtatttc agcatggttg
     4621 ccctctctat tcccatcccg aaggcatacc ctgtatactt ttcagaatca atgccgcagg
     4681 cctcgagaac attcggatcc accatgccac atccaagcag ctcgagccag cccgtaccct
     4741 tgcatacgtt gcatcctttt ccgccgcaga tgttgcagct tatgtccatc tcggccgaag
     4801 gctcggtaaa ggggaaaaag gagggcctca gcctgatgct gacatctttc ccgaacagtt
     4861 cggtggcaaa atagagcagc gtctgcttca ggtcggcaaa tgatacattt tcagcaacat
     4921 acaatccttc aacctggtgg aaaatacagt gtgcccgtgc tgaaacagcc tcgttgcgga
     4981 agactcttcc gggagaaatg gtacggatgg ggggctgctg gttttccatt accctgacct
     5041 gaaccgagga ggtgtgtgtc cgcaggagca catcatgagg atcggactct gtgccggatt
     5101 tcttttctat gaaaaaggta tcctgcatgt cacgggccgg gtggtcactg gcaaagttga
     5161 gcatggtgaa gacatgattg tcgtcttcta tctcgggacc ttccgacacg gtgaatccga
     5221 tacggctgaa gatgtcaatg atctcgttcc tgacaatgga tatggggtga cgcgttcctt
     5281 ccccgaaggg ataggcaggc atggtaagat cgactgatgg tccggcaacc tcggtcattg
     5341 ccaggcgctc cttgtgctcg ttgtatttgt ctgttgcaga ctgtttaaga tcattaagca
     5401 gctggccggt ttcccgcttc gcctcaggcg gaacgttcct gaatccttca aaaagcactg
     5461 atatcagccc tttcttgctc aggtaggcta tcctgaactg ctcgagtgaa ccctcgccgt
     5521 caatattgta ttcttcgatc tcttttttta gttctgctat cctctccttc atctctgaat
     5581 gccctgttat ttaatgattt tcaccttgac aatccttatg tcctgcactg gtctgtcagt
     5641 caaatcggta gctgtacccg caatggcgtc aaccacttcc attccctcca gaacctctcc
     5701 gaaaaccgta taggcgccgt caagaaaggg tgtgcctccg acggttttgt atgcgttcct
     5761 gtgatgttcg ggcatgacat aggtaccggc tttttccatg gcgtcatatg cttttatgat
     5821 ggccgactgc tgtatttcat cctcggtcat ttcctttccg gcctgttctg cttccctgcg
     5881 aagatccaga agggccttgt tgtagagaaa ctgccggcgg ttgtactcaa tgcgctctat
     5941 cgtggcggca agctcctcgt cattgtaaac cttaccctgc acgatgtaga actgcgttcc
     6001 ggaagagtta cgttcaggat tgacatcatc accggtcctt gcagccgcaa ccacgcctct
     6061 cctgtggaaa agggaatcat tgatctctgc cggaacagta taatcatcac tgatgagcgt
     6121 tgaatcctgg cgtgtggagc cgtcaccggt ctgtatcatg aaattcttta ttacacggtg
     6181 gaaagtgaca ccgttataaa aaccgctctc ggcaagtttg atgaagttgt cgcggtgcag
     6241 cggggtgagg tcggagagtg atatcacaat gtctccttcg gtggtctgta acacggcgcg
     6301 tacgcctttc ttgcttcctg ccgagcagcc ggcaattatt atggctgctg ccagtgtcat
     6361 gacgatgcgg aatgaagtcc tcattttctt ttaatttacg aatgacaaag atatatattt
     6421 gcagtaagag ttaagcatga acgatatacg caaacttgcg gggcagacgg taatatacgg
     6481 atttggaacg gttgtgccgc gattcctgaa ctacgccctg cttaccccat tttacacccg
     6541 tatttttggt cttgagcagt acggtgttgt tacggaactc tatgcatgga tggttctggc
     6601 gcttgtcatc ctcacctacg ggatggagac cacctacttc aggtttgccg gaaaaaaggc
     6661 gccggcggaa aaggtatacg gcacagcact gatcagcctc ttttccacct cagccctttt
     6721 cctgctggcg gtatcgctct gcataaatca ggtttcagga tttcttggtt atgagcagca
     6781 cccggagtat atcaggatgt ttgcctggat agtggccatc gacgcattct cggcaatccc
     6841 ctttgcatgg cttcggaaca acaaccggcc ggttgtcttc agcgccctga agatcataaa
     6901 cgttgttgta acaattgtca tagccttctt tttcctgaag atcgcacctt cactggcaga
     6961 aaaaggaagc acctggattt acaagatatg gaaccccgat ttccaggtgg gatatgtctt
     7021 tgtggcaaac ctggccggat ctgccattac tctcatatgt cttattccgt tcattttcag
     7081 gatgcagccg gtgtttgaca ggaaactgct gggcaggatg ctggcataca gctggccttt
     7141 gcttatagga ggtatggccg ggtcactaaa tgaagtactc gacaaaatcc tcctgcgcag
     7201 actgattggt ggcactgagg ggcttgccac tgtagggatg tacggggcag gatataaggt
     7261 gggagtactc atgtcacttt tcatccagat gtatcgcttt gcggcggaac ccttcttctt
     7321 tgaaaaggcg ggaagcaggg acgcgaagga aacctatgcc gtaacgatga aatacttcgt
     7381 gataacggca ctgatactat tcctgggaat caatctgtac ccggaggcag tccggatcct
     7441 catcggggat attttccgcg aatcactgat tgtggtgccg attgtaagca tgggatatct
     7501 cctgctgggc atcttcatca accagagcat ctggtacaag gttgacgaca agacaattta
     7561 cggagcatgg attacccttg tgggtgttgc cgtaacagtt gcagtgaacg tcattttcgt
     7621 gccggtgttc ggatatattg cagctgcctg ggctcacatt gcatgctatc tttcgatggt
     7681 catcgtctca tattttgtgg gacagaaata ttacccgata aagtatgaaa cagggaaaat
     7741 attcatgtac atagtcctgg cggtggcact gctgttcgtt tccatgctgc tggcaggcga
     7801 aaataaaata ctcaatacag tcattgacac tctgctgctt ttcattttct ttgccgttgc
     7861 tgaaatcagg gataaattct ttacgttatt gtttaaaaga ggttcatgaa gatcaggata
     7921 gtaaataagt catcacaccc ccttccggca tacgagactg agcactctgc ggggatggac
     7981 ctgcgggcgt ttaccggggg accggtcacc ctgaagccga tggagaggaa actcattccg
     8041 acaggccttt acattgagct gccggttggt tatgaagctc aggtaaggcc acgaagcggc
     8101 ctcgccatca ggcacggaat cacggtattg aactcacccg gcacaataga tgcagactac
     8161 aggggagaaa ttggagttat attgattaac ctgtcggaaa gcgaattcac aatcaacgat
     8221 ggagacagaa tagcccagat gataatttca cagtatgtga aggtggagct gacgggtact
     8281 gacagtatag aaatgaccgc caggggcgaa ggcggattcg gacattcagg gagaatatga
     8341 agaatacagt catactggta ctgctcctgc tggctgcggc tggatgttcg gaaaaggcgg
     8401 tttcgcctgt cctgacaagg gaccgggaag caagggccga caaccgttat gattatatgc
     8461 ttgccgaggc gctcaggcag aaatatgttg gcgaattaag cgaagccgca gtactgtttg
     8521 agaagtgcat cgagcttgac aggaacaggg cagtgcctta ctttgaactg gctcagatgt
     8581 actcagctgc aggaatgagc ggaaagtcgc ttcagtatgc ttcgaccgcc gcaaggcttg
     8641 agcccgggaa ttactggtac cagcttgcat gcggctccct cttcacccag tatgagatga
     8701 aagactctgc cctggtttat ttttcaaggg cactgaaggc cgacagcaag gcggtggaag
     8761 ttaacggcat cctcgccgga ctgtattcgg aaaaaggcga gacatccaaa gctgattcac
     8821 tgttcaaagt ccttgacagc gaaggcgctc ttacgccgga tatgtcactg atgatgatat
     8881 ctggcctgct tggcatgaat gagctcgacg aggcgtcgaa gagaaccgtt aaactcctgg
     8941 aacagcatcc tgaggagacc cggttcaggg cattgcttgc tgatatctac tatgagcagg
     9001 gtctgaaaga gaagagtgac agcatataca cggaaatcat cgaaaaggac ccggatgaca
     9061 tagaaacaca gttgctgtac ctgataagcc ttgtataccg gaaggaatac tcgggcatat
     9121 ccggtttcct gggcaatatc ttcagaagtg atgtggtgga acgtgaaaga aagattgccg
     9181 tggcgcgcag gctggccggc gattccgcct acataaagga caatgctgaa tcactgctcg
     9241 gaagcctgtt gatccttgag gagcaatacc ctgatgatga ggagattatt tcactacgcc
     9301 ctggcgtcta tgagaccacg ggcatgattg accttgccat acagagatat gaggaaatca
     9361 ttaagagtgt cagccagggc ttctggttca gggagagact gatgatactc tatgccggga
     9421 agaaggagta tggcaaactt tatgaactgg ccgctggtta ttcaagggat aataaccgta
     9481 gcctgctagg aaagatatat tacgccctgg ctgccatgga gctgaaagaa tatgctgttg
     9541 ccgacaatga gctgaagaag gcgctgattc ttgccggaaa caatgacgag ctgaagattc
     9601 aggttttatc acttgaaggc gatctgaaat acaggatgaa ggattatgat gcttcatatg
     9661 caagctatga agaggccctg aaactgtctc ctgatgatcc gctgctgctg aataactacg
     9721 cctacttcct tgccgaaggc aacagagacc ttaagaaagc cctgaaaatg gccatgaaag
     9781 tcatggaaac cggaagtgac aacccgacct acatagatac atatgcctgg gtgctctaca
     9841 agatgggaaa atacagggat gctcaccgtg aaatgctgag aattttcgaa ctgagcagtg
     9901 aaagagaccc tgaattactg gagcatatgg gatttgtcaa gaaagccctt ggcaaatgtg
     9961 aggaagctgt tatttactgg atggaagcac tggagaagga caataccaaa atatacctgg
    10021 aggaggagat aaagaaatgc atgaagcaat aaaatatgtt gcaataatat cgttcctttt
    10081 tctgctggca ggctgttctg ctttgaagaa ggagaagaca accggaccgg atgtccagcc
    10141 ggcggatgcc ggttatagtg cgataatagg caatgtggcc tcaaataata tttctgaaag
    10201 gggctttgaa ataaggaagg gcagcattga actggaaggt accgaagttg aaggaaaatt
    10261 tggtctgcat gcaaggctga acagcaaagg tgatttttat ggctccgtca gaggtcccct
    10321 gggaattgaa ctcgtcaggg ttctgatggt cggaaatgac atcgctgcaa ttgaccggtt
    10381 taacaggaaa atttacattg gtaaaaagga ccaggtccta aaaaagaatg gaatgccgtc
    10441 agattttatg agcattattt tcggcgacat gccttcagaa gacgaagtgg agctgatcag
    10501 atcggaaaaa aatgaaattg ttgttgcttc aggaaatggt gacttcagta gtgtcatctc
    10561 catttgcctt gactacatga aggtttgtag ccggaagatt aatgcaaaca cctctgatca
    10621 tgagatttac atgttattcg gagagtttgg ggagtcagga ggaatgaaat atccctctga
    10681 aatatccatg gaagagaaga aaaaaatgtt tcacgtgaaa ctttcaatag atgatttgat
    10741 ttacgggtat gattctgaca tagaattaaa cctgccatca tacaaaaggg agtcattttg
    10801 aaatatctgt tcctcatatt gtttcttgca gtctggtccg cttcggtgct ttcaggacag
    10861 agtcgctccg aactggagga gatgagaaag aagaatcttc aggaaattga atatgttgac
    10921 cggttgctga aaacgacggc aagccagaag actgagaacg tgaatgagct gagggtgatc
    10981 ggcagaaagc tgaatctccg tgaaaaactc atagatgagt atggacagga aataaatctt
    11041 ctggaataca ggattggttt gaatgccctg gcaacagaaa tgctggagca ggatctgaat
    11101 gagctgaaga aggattatgc aaattcaata ttgagcgctt acaaggccac caaaggcaca
    11161 cctgcactgg cattcattct ctcggcagca gactttaacc agggatacaa aagattgaaa
    11221 tatatacagc aggttgcaaa gtacagaaga aaggaatcag agactatcga aacgctttac
    11281 acacagcttc agggaaccaa ggaaaggctt gaaaaggacc ggaagaatgt aagtgacctg
    11341 aagaacaaag aggtaaggca aaagcagatc ctgcggcagg agcaggacag aaaggagaaa
    11401 ctggtaagca atctgagcag gaaggagaag cagttaaagc aggagcttga tgagaaaaga
    11461 agaattgcaa ggcagattga aaaggagatt gagcggctta tagaggctga gcgaaagaag
    11521 agcgagactg tgccgatgag tgcagaaatg aaggttatag gtgagtcgtt cggagaaaac
    11581 cgaggaagga ttccctggcc ggttgacagg ggtatcatta ccagtcattt cggaagtcat
    11641 cagcatccgg ttcttaagaa tgtaattgaa gataatatag ggatagagat aacctcgagc
    11701 ggtccgacaa aggcaaagtc tgtgttcaag ggcgaggtag gaggggtgtt tgccatatct
    11761 ggtgcaaata tggctgtaat aatcaggcac ggaaagtacc tgacagtgta tcaaaacctg
    11821 gtaaatgtcg ttgttaaggc aggagacagc gtatcactgg gtcaggttat cggtgacgtg
    11881 tatgtggata acggcgacgg cggaaaggca gagctaaaat ttatggtcta cgaagagaag
    11941 aaaaagcttg atccggaaca gtggcttaca aaaaaatgac taaattttgc aacaaataaa
    12001 taccctttca gttaaaaaat agtgataggg attcaggaat ggaaagcatt actattaatt
    12061 cagacattga aaaattgcgc gttgtagaaa cgctggttga tacgttgtca aaaaagcttg
    12121 gtatctctga cgaggtttac ggaaagatac tgatttctac agtcgaggct gtaaacaacg
    12181 caatattaca cggaaacaag ggtattgagg atagaactgt gacggtggat tttactgctg
    12241 acggaaatat gtttgaggtt accgttacgg accagggtga gggctttgac tacaatcatc
    12301 tgccggaccc tacggatccg gaaaatatcg agaaccttca cggacgcggg gtgtttatta
    12361 tgagaagcct ggcggacata attgagtata acctgccggg caatcaggta aagatgcggt
    12421 ttaaatactg attccgtgaa agtaagcttc cattacgata ttgaaaaatt cagactgcgc
    12481 gacagcagaa taatcaaaaa gataatcaat cgtatagtga cggatgcaag actcaggccg
    12541 ggcatcattg acgtaattgt gaccggtgat gagaaactgt acgagataaa ccgggagttt
    12601 ctgggtcata attactatac tgatataata acattcaatt acaatgaagc cggcacggta
    12661 aacggagaga tctacatcag tgctgaaagg gtcagggaaa actccgtcaa attcaatgtc
    12721 acgttcggct cggagatgag acgggtcatt tttcatggat tgcttcattt atgcggatat
    12781 gatgacagaa cagaggggga gaagaataag atatctgaga tggaagagat gtatctggcc
    12841 ttatcatatg ccgaatgatc ttcagttatg atataattgt cgttggcggt ggtcacgccg
    12901 gctgtgaggc tgctcatatt gccgcggtaa tgggtgcaaa aacgctgctt gttaccatgg
    12961 atatgaccaa gctggcccag atgtcctgca atcctgctat gggtggcatt gctaaaggac
    13021 agatagtcag ggaaatagat gctcttgggg gctcgtcagg aataataact gacaggtcaa
    13081 tgattcaatt ccgcatgctg aaccgaagca aaggtccggc catgtggagt ccgcgggcgc
    13141 agtgtgacag gatgctgttc agcagggagt ggcgaaagct cctggagcag acagagaacc
    13201 ttgcaatatg gcaggaggaa gcaagcggca ttatcattga ggatggcgtt gtaaagggag
    13261 taaccactac cttcggaaca gagttacgtg cagacgctgt gatactgaca aacggcacat
    13321 ttcttaacgg gctgatgcac gttggtttca aaacaatgtc gggcggcagg tcaggcgacc
    13381 cggcttcacg cggactgagc gaacagctca gggaacttgg ctttgaggtg gagaggctga
    13441 agacaggtac cagcgcaagg gttgacggaa gaagtgttga tttctctgca atgacagagc
    13501 agaagggaga cgatgatggg cgatgctttt cgtatctgca cgacagggtt gacataaagg
    13561 atcaaaggag ttgctacata acacacacga atgaaaaggc acatggcata cttcgggaag
    13621 gactgaagtt ttctcccctg tacacaggaa ggataaaggg aaggggaccg aggtactgcc
    13681 cgagcgttga ggataagatt gtgacctttg cagacaagga ggcccatcag ctgtttcttg
    13741 aacctgaggg atgggaaaca aacgagtact atataaatgg ttttgcaagc agccttaagc
    13801 tggaaacaca gatctctgcg ctgagggaaa taaaaggcct ggagaatgtg attatataca
    13861 ggccgggata cgcaatcgaa tacgacttct tccagccgac ccagctgaag cactcgctgg
    13921 agaccaaacg agtaggagga cttttctttg ccggacagat caacggcaca acaggctatg
    13981 aagaagctgc agcccagggc ctggtggcgg gaattaacgc tgtgcagaag gtaagaggaa
    14041 aggacccttt cattttaaaa cgtgatgaag cctacatagg agtactgata gatgacctgg
    14101 tcacaaaggg cattgatgaa ccttacagga tgtttacctc cagggctgag tacaggatac
    14161 tgctgcggca ggacaatgca gacgagcgtc tcacacccat ggctgttgaa cttggaactg
    14221 cagagccggg aaggattgaa agacttgtgg aaaaataccg gatggttgat gagcttatgg
    14281 tgtttctgaa gaattgcagt atttcacccg aagagatcaa cggattcctg aaggaaaagg
    14341 atacggcgga aattaatcag aaaataaagg ctattaacat tgcgacacgg ccgcaggtaa
    14401 gtcttgaaga gctattggag caaataggcg gatcaggcaa actgagttac gccgggtctg
    14461 taagaaaaac tgagattatt gaagcagctg aaatcaagat caagtattcc gggtatctcg
    14521 agagggagag gggcatagcc gaaaaaataa tgagactgga aggactgaag attccggaag
    14581 agataaacta tgaagagctg gcttcaatat ctacagaggg aagacagaaa ctgtcaaaaa
    14641 tcaagcctga taccattggc caggcttcca gaataagcgg tgtgtcttct tctgatgttt
    14701 ccattctgat aatgtatctt ggcagataat aatcaatgtt ccacgtggaa cattatttct
    14761 ttatacatta tgaatatcgc aggaaaaatt attgatgtgc ataaaaagcg catttatccg
    14821 gggattgtca ctattaaagc cggcaaaatt gaatctgtaa ccgaaaccga aaggagtcct
    14881 gatcatttca tcatgcccgg actggcagat gcgcatgtgc atattgagag cagcatgctt
    14941 gtgccatctc attttgccgt ggctgccgta aggcatggga cggttgcagt tgtaacagat
    15001 ccgcatgaaa tagcaaacgt ccttggaaag gaaggagtaa aatttatgct ggataatgcg
    15061 gaaaacgtac cggttaagat agttttcgga gctccctcct gtgttcctgc cacatcactt
    15121 gaaagcgccg gagcaaagat cggatctgca gatatcagag aactgatcga agaaggaaaa
    15181 gtaaaattcc tggctgagat gatgaacttc ccgggtgtta tctatgatga tgctgaagtt
    15241 caccacaagc tggaagctgc tcttaatgca ggcatcccga tagacggaca tgctccggga
    15301 ctgacaggag aagaattaaa aaaatatatt tctgcaggaa tcaccactga ccatgaatgc
    15361 agctccctcg aagaggccct cgaaaaaatc tcaggcggaa tgaaaatcct gatacgcgag
    15421 ggaagtgctg ccagaaacct tgaagtactt gctccactga tcagttatta ccccgaaaag
    15481 gtaatgctct gcaccgatga cctgcacccc gagacactgc ttcgcggaca tatcaacaag
    15541 ttagttgcgc ggctgatttc aatggattat aatttatttg acgtcgtcag ggctgcatcc
    15601 gtaaatacct atgaacacta ccggatcagc gccggaactc tcagacccgg agatccggcc
    15661 gattttataa ttgttgatga tccgaaaacc atgaatgttt tgcagacctg gatcgacgga
    15721 aaatgcgtat tcgacggcga taaacctttg ttttctccgg gagagatcgt taaaataaac
    15781 aaatttaaat gcacacggct tctgcccgat gagatcaggg tgatcaacga aggtggcaag
    15841 attaacctga tcgtcgccgt aaacggcgaa cttacaacag atgcagatat tgtgcaggca
    15901 ggggaggggg agtttgtcgc cgccagaccg gaagaagaca tacttaaaat tgttgtcaag
    15961 gaacgataca atgaccggcc accggctgtc gcgtttatca ggggtttcgg aatgaaacat
    16021 ggcgccatgg catcatcagt tgcccatgac agccacaata tcattgctgt tggcacaaat
    16081 gaccgtgata ttgtcaacgc catcaacctg attgtggatg ccggaggcgg tatggcggtt
    16141 acttcggaag acggggcgga aatactcaaa ctgccggttg ccggcatcat ggctgacctt
    16201 cccgtaaccg caatggcggc ccggtatgaa aagctctctg agacggtcag gagcctggga
    16261 tgcaggctgg atgcaccttt catgacactc tctttcatgg cgctgctcgt cattccgaag
    16321 atcaaaatga gcgacagggg actatttgat ggtctgacat tcaagcatat acctcttttt
    16381 gtgcctgatc cctacagcaa gtacaagacc tattaaactt gcccgcagac actgtgcctt
    16441 catggaatta agtttcagtc aatgaggtaa ttgaacaatc tcaattcatc tctttaatgc
    16501 cttgaagcca tttgtcaggg cataatttct aactttgccc gttgcccgac ggggtcctgg
    16561 ccttcctaca tggcaccgtc agcgcggcgg cagaaatgaa taactgatat gtcagcagtc
    16621 gagaccttaa aatcaacggt atccgcactg cccgacaaac cgggcgtata ccagttcatt
    16681 gacgcttccg gaaccattct gtacgtcggc aaagcccgca gtctgcgcaa acgcgttggc
    16741 tcatacttca caaagaacca gtcagggaaa acccgggtca tgctctcccg ggctgccgat
    16801 ctccggcata ttgtggtaga caacgaatcg gaagcgctgc tgctagaaaa cagcctcatc
    16861 aaaaagcacc agccgagata taacatcttg ctcaaggatg acaagacgta cccatggata
    16921 tgtgttaaaa atgagcactt tccgagggtt ttcatgaccc ggaagctgat tgctgacgga
    16981 tcggcttact tcggtcccta tacatccgtt ccggcagtta aaaccctcct ggaactgatc
    17041 cggcacctgt tccagatacg cacctgttca ctgcccctca actcaaggtc tatttctgaa
    17101 ggtaaattca aggtatgcct ggaataccat atgggaaact gcaaagcgcc atgtatcggc
    17161 ttcatcagtg aggaggagta taaccttcag gctgacagga taaaagagat aatcagggga
    17221 gatctttccg gggtaacagc ataccttgag tcacagatgc ggcagtatgc cggagatctg
    17281 aaattcgagg aagctcagaa gatcaaggaa aaacttgagc tgctgtcgag ataccgcagc
    17341 aaatcagtgg ttgtcaacac ctccgtaaag aatgttgacg tatttggatg ctctgccgga
    17401 gaaggatcaa tgtatatcag ttacatgaag gtggtcaccg gcgccatagt tcagtcatgg
    17461 tccgttgaac tgaaaatgag gatggaagag gagaaggagt cgattctctc aaccgcagtg
    17521 acagagataa ggcagcgggt gtcaagtgac tctccggagg tgatagttcc ttttatgcct
    17581 gacattcagc ttgataaggt aaaatattct gttccacagc ggggtgacag gtataaactg
    17641 cttgatctgg ccaaccgcaa cgctgtgttt ttcagactgg aaagacaaaa gaaaaccgca
    17701 gaccggtcga aagagaaccg cactgaaaag aacctggaaa agatgaaaaa ggatctgcac
    17761 atgccggcgc ttcctcttca cattgaatgt tttgacaact ccaacataca gggtgaatca
    17821 gctgttgcag cgtgtgttgt attccggaac ggcagaccca gcaacaggga gtatcgccgc
    17881 ttcaacataa agactgtcac aggcccggat gattttgcct caatggaaga ggtcattttc
    17941 cggcgttaca ggaggatgct tgatgagcag acgccgcttc cccagctggt ggtgattgac
    18001 ggcggcaagg ggcagctgtc atccgccgtg aaaagccttg aaatgttggg catcaggggc
    18061 cggctgacgg tgataggaat cgccaaaaaa cttgaggaga tatatttccc gggcgattcc
    18121 gttccgcttt accttgacaa gaacagcatc accctgaaaa ttatccagca cctcagaaac
    18181 gaagcccacc gattcggaat aaatttccac agggaaaaaa gaagctccgt catgaaccgt
    18241 tcaatcctcg atgaaattcc cggcataggg gagaagacaa aggagcggct tctgaaaaaa
    18301 ttcggttccg tcagaaatct ctctggagca accgcggcag accttgaaaa ggttgttggc
    18361 gccaaaaaag cctcacttct ggccgctgcg cttaaaaatt gattttattc cacgtggaac
    18421 aaatttattc cacgttgacc ataagacaca gttatacagc acgtaagagc accttacctg
    18481 cacagtcaac ttcacgagaa ttgaatattt tccctcaggc gggcagtccc ccgaatattt
    18541 tcttcagaaa acggtgaaag gagtggatcc cgtccggttt ccagaacatc attatgcact
    18601 ggtgatggat caggttgaca tcttcatttt ccatggcggc tattgtctct tttgattctg
    18661 ccccctgctg aatccagaga tcttttattc cgtgtgcaat tgcctctttt gccacggcaa
    18721 gagtttcctc tttcggagtc ataaaaatca agccacggat gccggccggc agttcgctga
    18781 ccgatctgta gcattttact ccgtcaattg tttcggcggc aggattgacg ggtactacgt
    18841 ccatcccctt cttgcgcagg tcccggaaca tggcctgccc gaatttcttc ggatctctcg
    18901 agacgccggc tatagcatag gtgccaagcg agagaaagcg gtttacatct ttcatttcct
    18961 tctca
//
LOCUS       DAOPXU010000051        18882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_3055_length_18882_cov_12.723164, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000051 DAOPXU010000000
VERSION     DAOPXU010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18882
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3055_length_18882_cov_12.723164"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..3114)
                     /gene="ccsB"
                     /locus_tag="PKK03_06750"
     CDS             complement(52..3114)
                     /gene="ccsB"
                     /locus_tag="PKK03_06750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HNX84137.1"
                     /translation="MKALRFLISPAMMGVLFIALAASMAAATFIENDFGQEAARAAVY
                     NTRWFELLFLLLALNLVGQIYTFRLWRREKLTVLLFHAAFIVMVAGAAITRFTGYDGM
                     MHIREGYSSNVTFSSGNTLTFELTGNDGAILDSHSAPLNISGGRHGKYRKSLEADGAK
                     AALTFERYVAGAVKTVQETPGGKPMVSLLFTPDMVSSETLVLALGESAMIGDMKAGFG
                     TPADISVTNDGNSFFISSSTGLRSTVMQEMKSAEYPADSLVPLMAGTVYTAGSYRIMP
                     QMMTLSGTIVPSATAAGKGPGGALEFSLKGDGFQERIWLWDDGGEERSVWNGNAGRLT
                     ARVSYGASGRELPFSLRLDDFVVERYPGSSSPSGFRSRVTLSDPEHGTGMPYDIYMNH
                     ILKYRGYRFYQSSYDNDELGTVLSVNHDPAGMYTTYTGYGLLFLFIILSLLNRKSFFR
                     TVAPGHWTSNYRKPAAALVLLLLTSLSPLKSQQLVIERKAADRFGAVLAQDQKGRTKP
                     LYTISSDILRKVSRENSFDGMSPMQVFLGCSLDFYHWQNVPLIKVSNEELRRELNLKG
                     EKAAFSDIVKMGEGGGYLLASLVDKAYAKSVTERTKFDKEVIKVDERVNICYMMSRGD
                     FMQIFPLRDGTDHWGSAEEAVRHGISREDSLFVMGVVPLWAQSVTSVGRPMAPPADYI
                     TALTNYQLRYAGYELPSEGKVKAELFYYRARIFERLFPWYATVGLIMIITIITMIIRG
                     RELRGAVLRVLAGLVAAGFLFHTIGLGIRWYISGHSPMSNGYESMLFISWVTLLAGLI
                     FSRKSLLTLAATSVLGGLTLMVAHLSFMDPEITNLVPVLRSYWLTLHVSVITGSYGFL
                     GLGAILGIVVLVMMLFIHEGNRARISAVIDELTVINYRTLTLGLYFLTIGTFLGAVWA
                     NESWGRYWGWDPKETWSLITIIVYTLVTHSRMMPGMKDTYIFNLLSLFAFSSVLMTYF
                     GVNYYLSGLHSYAGGDAVPVPAFVYVAVAVLISLAVASGYAYRKALRR"
     gene            complement(3135..3830)
                     /locus_tag="PKK03_06755"
     CDS             complement(3135..3830)
                     /locus_tag="PKK03_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNX84138.1"
                     /translation="MYTDNKIISCEFCSRCWSNFKTLSAEELELVNKNRYEATFKPSE
                     IIIKQGSPSSNAIFLLSGLAKVYMEGYAGKNLILNLAESKTLLAGPGVHVNSRYCYSV
                     AAITHVQACFINFDVIRNVISANPGFAAGFIEDLSDKALRLHQKMLNLTQKKMHGRLA
                     EALLYFSDSIFHSDEFEMVLSRQELGEMTNMAKESVVRIIGEFENENIIHATPRSVRI
                     LNREKLLVISEKG"
     gene            4033..5100
                     /locus_tag="PKK03_06760"
     CDS             4033..5100
                     /locus_tag="PKK03_06760"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNX84139.1"
                     /translation="MKKSLKFAALVVSAGLIMASCEGPMGPVGEDGPKGDKGDPGTTL
                     GCAECHNPGSPMSAFSAQWSESTHAKGGNAAYANRAGCVQCHTSQGFLEYLAEGSTAN
                     IAVPDNPMQINCATCHKIHETYSAADWSLTKPGAQTLEVKYAGADVIWDKGSSNQCAF
                     CHQSRTVSPAPAVNGADFNITNTRMGPHHGPNTNLILGKTPFELPGEAHPTTNPHSTA
                     NGCVTCHMSAPYGYLAGGHNMGVWYDSHGTETLLKTGCTTCHTNTTTLDTKIADLKSD
                     ITEKMASLEAQLIAAGIYNSATGLAKTGTFKANAVLAYLNYNTVKEDRSNGIHNPGYI
                     STLLDNSIATMEALGYPVPGK"
     gene            complement(5167..5583)
                     /locus_tag="PKK03_06765"
     CDS             complement(5167..5583)
                     /locus_tag="PKK03_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNX84140.1"
                     /translation="MKRTFKTISVVLATALFAFASVTANAQQKKGEPWVIPAEYKKMA
                     NPVAKDDASVKAGMVSYNKNCASCHGKTGLGDGPKGRMCKTFPGDFSGAAFQGFTDGE
                     MFYQTKMGRGEMPAYDKKIPDNEIWSIVNYMRTLKK"
     gene            complement(5613..6233)
                     /locus_tag="PKK03_06770"
     CDS             complement(5613..6233)
                     /locus_tag="PKK03_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNX84141.1"
                     /translation="MSKNFYHYPVWVRLWHLTNAILCLFLIVTGFSMLYSDPDRALVV
                     KFQRAVSIHNVCGVLLTISYTAFVIGNIFTENGRHYLIAIRGMAERLWKQARYYGFGY
                     FKGEESPFPVTGNRKFNPLQQVSYVGVMYFVLPMLFVTGWALMFPEFILKRFLGFSGV
                     FLTDQFHVILGFLVVIFLFIHLYVSTMGKSPLSNFRSIITGWQESH"
     gene            complement(6226..7230)
                     /locus_tag="PKK03_06775"
     CDS             complement(6226..7230)
                     /locus_tag="PKK03_06775"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNX84142.1"
                     /translation="MIIRKLLLLLYFLPLLASPDAAGQETDTVKSVRGASNSLYAEDN
                     ENNCLVCHGRLVYSLTDTTTGTTRKQLMSEHNLVVPDMFYNSVHWSFNCLDCHAEGFK
                     TFPHPLETRFESSWGCIDCHGYDPNYAQYRFEEIDAEYQRSIHYTATEGLITCWKCHD
                     PHYYNPLARQTTGGREYIIRSNEMCLRCHGNSDVMGLINDENVSIVLPKHEWLPDVDR
                     HMEAVRCIDCHTRMNDSVLVAHEVMPADSAVQGCAECHSQNSILMGTLYKYVAQETRV
                     EKGFLNGVILRNESYVIGANRSKFISFTGLFLIGMTIAIAMVHTFFRIIIKRKKIVHE
                     "
     gene            complement(7244..8080)
                     /locus_tag="PKK03_06780"
     CDS             complement(7244..8080)
                     /locus_tag="PKK03_06780"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HNX84143.1"
                     /translation="MKKIVISVILALITIPVFCQDSEDYPVRVFETSILIDNQTVATP
                     FKGMLEFEIHHRFGTVNNALDDLFGLWAPANIRLGLNYGITDRLMIGIGTAKNYKAQD
                     LAVKFAILQQTSSGSVPVSLSYYGNMGINLLNQNTFGPAHDWREIHRLSYFNQILVAR
                     QFGTKLSLQLAPTFIWLNAVEVGYKNAHYGISAGARYNVTGSHSIIAEYDQLFSSQKD
                     ESLNAKPQLALGWEIGTATHAFQIFFANYKDILGQYNFVYNQNSISDGKFLIGLNVTV
                     RF"
     gene            complement(8109..8474)
                     /locus_tag="PKK03_06785"
     CDS             complement(8109..8474)
                     /locus_tag="PKK03_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84144.1"
                     /translation="MKRKAWITRAFAAALVVAGAISLSSCEQYSWAKQEVPQDLTVSF
                     SETLQPFCQGCHGAWTVDRTYDKLSANVDTVTPASSRVLSIHSSITAFGSQMLQIDTL
                     LIPATDVIKLWATQGAEKN"
     gene            complement(8711..10417)
                     /locus_tag="PKK03_06790"
     CDS             complement(8711..10417)
                     /locus_tag="PKK03_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNX84145.1"
                     /translation="MANFYTDNKDLRFHLKHPAMEKIVRLKEMDYREREKYDYAPHDF
                     EDAMDNYDRVLEIIGAICAEVIAPNAEDVDHEGPTVADGHVTYARGTRENHEALTRAG
                     VIGMNFPRQYDGLNFPMVPYVMAAELVSRADAGFANIWGLQDCGETILEFATDEIRQE
                     FLPRFNKGATAAMDLTEPDAGSDLQAVQLKAHYDESGGTWLLNGVKRFITNGDAEISL
                     VLARSEEGTSDGRGLSLFVYDRADGGVIVRRIENKMGIKGSPTCELVFRNAPAKLVGE
                     RRMGLIKYVMSLMNGARLGVGAQSVGIAQAAYEEARKYASERKQFGKTIDQFPAVYEM
                     LTLMRVRLEAMRSLLYETARYVDTYKAWSFIAAERPLTKEEREDLKNNTRLADIYTPM
                     VKLISSEWCNRIAYDAVQIHGGAGFIKDFPVERLYRDARITSIYEGTSQLQVVAAIRG
                     VTTGGYLAQIREYEKKELRPELDHLKKELVSLAGDYEMRVAQVVSAGNNELTDFHARR
                     LVEMAANIVMGYLLLHDAQRDAGYTSSAELFISFAVSENRVRSEYIGSFEEKALGLYK
                     VS"
     gene            complement(10453..11472)
                     /locus_tag="PKK03_06795"
     CDS             complement(10453..11472)
                     /locus_tag="PKK03_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNX84146.1"
                     /translation="MNNLFVFCELDEGRPADVSLELLTRGRSLANTLGCRLEAVVLGS
                     GLDGIENELFPYGADVVHVADDPVLEHYRTLPYTSVITGLFRQEQPGVALMGATSTGR
                     DLGPRVSSALHSGLTADCTSLEIGDHYVKKTDTTYKNLLYQIRPAFGGNIIATIINPD
                     HRPQMATVREGVMKREIVNRNHRGELKKIDVASFIRPEDLAVEIIERHMEARKVDIKS
                     AQIIVSGGYGMGSKENFALLYELASVLGGEVAASRAAVDAGYAGHERQVGQTGVTVRP
                     KLYIACGISGQIQHTAGMNQSGKIIAINNDPMAPINSIADYVINGDVAEVIPKLIKYY
                     KANAR"
     gene            complement(11496..12359)
                     /locus_tag="PKK03_06800"
     CDS             complement(11496..12359)
                     /locus_tag="PKK03_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNX84147.1"
                     /translation="MNGIKIVVLAKQVPDTRNVGPDAMKADGTVNRSVLPAIFNPEDL
                     NALEQALRIKERYEGVTVTLLTMGPVRAAEIIREGLYRGADDGVLLTDRAFAGSDTLA
                     TSYALALAVHRLAPDLVIGGRQAIDGDTAQVGPQVAEKLGWPQVTYAEEMHFEGEKLI
                     IRRRLERGVETVSAPLPLVVTVNGTAPACRYRNARLVMKYKYARTDTELQAESSDYIA
                     AGRSSVRITEWGVDAIGADPEQLGLSGSPTKVKEIENVILQAKESKRLTSSNADVESL
                     VRELLENHTIG"
     gene            12615..13529
                     /locus_tag="PKK03_06805"
     CDS             12615..13529
                     /locus_tag="PKK03_06805"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX84148.1"
                     /translation="MKGRPYAALVLSMIFWSFSFVWFKVANVDYDPITIVFIRLCIAI
                     LFLTGFLWITRRFTAIKKGDRKYFFLLALFEPFLYFLGESFGLTYVSSTVGSVIISTI
                     PVFAVIFAWVIYRERLKLINYMGVILSFMGVLIFITNSTGNIMMNPKGLALMFLAVVS
                     AVGYNMVLHRLAHEYDPVTIVNIQNIIGAILFLPLFLVFDLKDLLATGIVAKSFGSII
                     LLAIFASCGAFVLFAYSVRYVGIARANIFSNLIPVFTAIFAFFIVGDRLTARNGVGMA
                     VVIAGLFLSQARKNRKAVVDTDFAGRSA"
     gene            13590..14675
                     /gene="porQ"
                     /locus_tag="PKK03_06810"
     CDS             13590..14675
                     /gene="porQ"
                     /locus_tag="PKK03_06810"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HNX84149.1"
                     /translation="MIQRMRHRAPNLLSGIAVLRRWAVVLLFLPALSGQVLKGQTGGD
                     NTYEFLNLSHSAFANATGGINVASRRSDLTLPYFNPALLDASMDNDISLSFSSWFAGI
                     RYGYASWAHSTATAGNFAGGISFISYGTFTEADEAGNITGSFTAAEYGFNIIWSWQID
                     SSFSLGVNLKPVISNLERYFSAGLCADIGATYRNERMLLDAGLVIRNAGLLLVGYTAD
                     GGEPLPFEIIAGATKRLAHAPFSFSLTLRHLEKPDLTYDYDPEDDAGPGGLGEDILRH
                     IVLGVEVLPSDAFWIGAGLDYRRRAEMRTGQRTGMAGFTAGFGIRTKAFELAFAHGAF
                     HPAGGSNHITVTLRPEIIFKKTEHAEP"
     gene            14708..15412
                     /gene="cmk"
                     /locus_tag="PKK03_06815"
     CDS             14708..15412
                     /gene="cmk"
                     /locus_tag="PKK03_06815"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNX84150.1"
                     /translation="MTRKKRKIIITVDGYSSCGKSTFAKAIARELGYIYIDSGAMYRA
                     VTLFALRRGLAGNGIADEAGILNALPEITITFRPDSETGFSATYLNGEKVEREIRDRE
                     VSDNVSPVSTIAGVRARLVELQRSLGAGGGVVMDGRDIGTVVFPAARLKIFMTARPEV
                     RAQRRYLELVSKGLPADFDAVMKNISERDRIDSSREVSPLRQADDAVVLDNSDMTPEE
                     QMVWFRELYKKRINES"
     gene            15402..16277
                     /locus_tag="PKK03_06820"
     CDS             15402..16277
                     /locus_tag="PKK03_06820"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNX84151.1"
                     /translation="MKVDIEQSSGFCFGVENAVAIAEDALRNGETVYCLGEIVHNDIE
                     VSRLTGLGLKAITYEEFANLRDCKVLIRAHGEPPSTYAKARENNITIIDATCPIVRSM
                     QEKIRKVAELSRDERGQIVIYGKEGHAEVIGLMGAAGPRGILVTGKGDLHRIDYTRPV
                     FLFSQTTKSKVQYEVVAEIIRLNIEKVSPDHSSSNLKVHNTICRQVSGREPRLREFAM
                     KHHVIIFVSGRNSSNGRMLFEVCLKVNPRSHFISSPDELNPEWFRGAASAGVCGATST
                     PRWLIQEVAELVAAM"
     gene            16349..17329
                     /gene="pfkA"
                     /locus_tag="PKK03_06825"
     CDS             16349..17329
                     /gene="pfkA"
                     /locus_tag="PKK03_06825"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNX84152.1"
                     /translation="MGKIKKIGVLTSGGDAPGMNAAIRAVTRTAIYNGLEVVGIRHGY
                     MGMINANFKPLYAHTVSDTIQRGGTILKTARCDAFRTPEGRKVAYDNLRVAGIDGVVV
                     IGGDGSFKGASLFNEEHNIPFVGVPGTIDNDIYGTDYTIGYDTALNTVVEAVDKIRDT
                     ASALNRMFFVEVMGAEAGHIALYSGIASGAEAIIMPEIKGEARDINKMIETGNKRKKS
                     SNIIIVAEGDEEGGAIALAERLKPQLKGYEVRVAILGHLQRGGSPSALDRVNASRLGN
                     AAVEALLDGQQSVMVGLQNDEIVLVPFRKAVKQHKGLNQHLVDIIDILNV"
     gene            17350..18462
                     /locus_tag="PKK03_06830"
     CDS             17350..18462
                     /locus_tag="PKK03_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNX84153.1"
                     /translation="MGPSEVYFTDMHSTSSLNLLKKLERLVKAAGFEKLDFERKMTAI
                     KVHFGEPGNLAYLRPNFAARLASMVKEKGGKPYLTDCNTLYSGERTNAPDHLDAAFSN
                     GYNPLSAGCHVIIGDGVKGTDYREIEVNLDYTQTAMIGTAIADADIIITLNHFKGHEQ
                     TGFGGALKNLGMGCASIGGKLFQHSGNPPFIYQKNCTGCNICVISCAHDAVHLGEDRK
                     AFIDNDKCTGCGQCVAVCQFDAARVQWSSEGDVLSKKVAEYAFAVVKDKPALHINFII
                     DVSPDCDCWGHNDRPLVEDIGIAASTDPLALDKACADMVMEAPAAWHLREKDNDNDLR
                     GEDKFRIAHGSTDWNTGLLHGEKIKLGSLSYKLIKI"
     gene            18459..18848
                     /locus_tag="PKK03_06835"
     CDS             18459..18848
                     /locus_tag="PKK03_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX84154.1"
                     /translation="MKDPSSSKRMAILIVEDDPHSQLYFTKLLSGMYDTAVAESAAEA
                     WDQLHDRKFDLVLMDISLKGDEDGLSLTRRLRVEDGYGTLPIVAVTAYAFPDDRKRAM
                     EAGCTDYLPKPVSSNDLHRKIAELTLK"
BASE COUNT         4572 a         4965 c         4794 g         4551 t
ORIGIN      
        1 cctcccggct cgccgtccga accccggcat tcagcctttc ctgacgaccc cttatcgcct
       61 cagcgctttc ctgtatgcat atccggatgc aacagccagg ctgatgagca ccgccactgc
      121 cacgtagaca aacgccggta ccggcactgc atcgccgccg gcataggagt gcaaccccga
      181 caggtaatag ttgacaccaa aataggtcat cagcaccgac gaaaacgcaa acagcgaaag
      241 gagattgaat atgtacgtgt ccttcatccc cggcatcatc ctcgaatggg tcacgagggt
      301 gtaaacaatg atggtgatca gtgaccatgt ctccttcgga tcccaccccc agtaccggcc
      361 ccatgactca ttggcccata cagcgccgag gaaagtaccg atggtcagga aatagagccc
      421 cagggtcaga gtcctgtaat tgataaccgt aagctcatct atcacggcac tgatcctcgc
      481 cctgttacct tcatggatga acagcatcat cacaagaacg actatcccga ggatggcgcc
      541 cagccccagg aaaccgtagc tgcccgtgat aacggagaca tgcagggtaa gccagtatga
      601 ccgcaacacg ggaaccaggt tcgttatctc cggatccatg aagctcaggt gagcaaccat
      661 cagtgtcagt ccgccaagaa cagaggtggc tgccagggtc agaagtgatt tcctgctgaa
      721 gatcagcccg gcaagcaatg tcacccatga gatgaacagc attgattcat aaccgttgct
      781 cattggcgaa tgcccggaaa tataccacct gataccaaga ccaattgtat ggaacaggaa
      841 accggcagcc accagtcctg caagcacccg gaggactgct cccctgagct ccctgcccct
      901 gataatcatc gtaatgatgg taattatcat tatgagtccg acggtggcgt accatgggaa
      961 aagcctctca aatatcctgg ccctgtaata gaagagttcg gcctttacct tgccctctga
     1021 aggcagttca tacccggcat aacgaagctg gtagttggtg agcgcggtga tatagtctgc
     1081 cgggggagcc atcgggcgcc ccactgatgt cacagactga gcccacagcg gaacaacacc
     1141 catcacaaag agagaatcct cccggctgat gccgtgcctg acagcctcct ccgcacttcc
     1201 ccagtgatca gtgccgtcgc gcagcggaaa tatctgcatg aaatctcccc ttgacatcat
     1261 atagcaaata ttgactctct cgtccacctt gattacttcc ttgtcaaact tcgtgcgctc
     1321 agtcacactc tttgcatagg ctttgtcaac aagtgaagca agcaggtagc cgccgccttc
     1381 acccatcttt acgatatcgg agaaagccgc cttttcgccc ttaaggttaa gctcacggcg
     1441 gagctcctcg tttgaaacct ttatgagggg cacattctgc cagtgataaa aatcaagcga
     1501 gcatccaagg aatacctgca tgggtgacat cccgtcaaaa ctattctccc tcgacacctt
     1561 cctcaggatg tcactgctga tggtatagag gggttttgtc ctgcctttct gatcctgtgc
     1621 cagcaccgca ccgaacctgt ctgccgcctt ccgctctata accagctgct gactcttcag
     1681 cggtgacagg gaagtcagaa gaagcaaaac aagggcagca gcaggtttgc ggtagttcga
     1741 ggtccagtgt ccgggagcca ctgtgcggaa gaatgatttc ctgttaagaa gtgagagtat
     1801 tatgaaaagg aacagcaaac cataaccggt gtaagtggtg tacatccccg ccggatcatg
     1861 gttgacagac aggacagtgc caagctcgtc attatcataa gacgactggt aaaaacggta
     1921 accgcggtat ttcaggatat gattcatgta aatgtcataa ggcataccgg tgccgtgttc
     1981 cggatcgctg agagtcaccc tgctcctgaa tcctgacgga ctgctggagc cgggataccg
     2041 ttccacgaca aagtcgtcaa gcctgaggct gaagggcagt tcccttccgc tggcgccgta
     2101 actcaccctg gcagtaagtc tgccggcatt accgttccat accgaccgct cttccccgcc
     2161 gtcatcccag agccagattc tttcctgaaa accatcacct ttaagggaga actccagcgc
     2221 ccccccggga ccttttcctg ccgcagtggc agagggcact atggtaccgc tcaaggtcat
     2281 catctggggc attatcctgt agctcccggc agtgtaaacg gtgcctgcca tcagtggcac
     2341 aagagagtca gcagggtatt ctgccgattt catctcctgc attaccgtcg acctgagtcc
     2401 cgtggatgaa ctgataaaga aagagtttcc gtcatttgtc acggagatgt ctgccggggt
     2461 tccgaaaccg gccttcatat cacctatcat cgccgactct cccaatgcca gaaccaaagt
     2521 ttcgctgctg accatgtccg gtgtgaatag cagggatacc attggttttc ctccgggagt
     2581 ctcctgcacc gtttttacgg cgccggcaac gtacctttcg aaggtcaggg cagcttttgc
     2641 cccgtcagcc tcaaggctct tcctgtattt gccatgccgg cctccggaga tgttcagagg
     2701 tgctgagtga ctgtcaagga tggcgccatc attccccgta agctcgaatg taagagtgtt
     2761 gccggaggag aatgtgacat ttgaggagta tccctccctt atatgcatca tgccgtcata
     2821 accagtaaac ctggttattg cggcgcctgc caccataact ataaatgctg catgaaaaag
     2881 gagaacggta agcttttccc tgcgccacag cctgaaggtg taaatctgcc cgaccaggtt
     2941 cagggccagc agcaaaaaga gcagctcaaa ccacctggtg ttgtatactg ctgccctggc
     3001 agcttcctgt ccgaaatcat tctcgatgaa cgttgctgca gccatcgatg ctgcaagagc
     3061 aatgaagagt acacccatca ttgcaggaga aatcagaaac cttaatgctt tcattaaatc
     3121 aatttgatgc aaatctatcc cttttcggaa attaccagca gtttttccct gttgagtatc
     3181 ctgacagacc tgggagtggc atgaataata ttttcgtttt caaactcacc gataattctt
     3241 acgacgctct ccttggccat attggtcatt tcgcccagtt cctggcgtga aagcaccatc
     3301 tcaaattcat cggaatggaa aatggaatct gaaaaataaa gcaatgcttc ggcgagcctc
     3361 ccgtgcatct ttttctgggt caggttaagc atcttctggt ggagcctcag tgccttgtcg
     3421 ctgaggtctt cgataaagcc tgccgcgaag ccggggttgg ctgaaatcac attcctgatg
     3481 acgtcaaagt tgataaagca ggcctgcaca tgtgtgatgg ccgccacgga ataacaatag
     3541 cgggagttga catgaactcc cggaccagcc agcagggttt ttgattctgc caggttgagt
     3601 atgagattct tccctgcata cccttccata tatacttttg ccagtcctga cagaaggaat
     3661 attgcattgg aagatggcga gccctgtttg atgattatct ccgatggttt gaaggttgcc
     3721 tcatatctgt tcttgttgac gagttccagt tcctctgcgg agagggtctt gaagttcgac
     3781 cagcaccttg aacagaattc acagctgatt atcttgttat ctgtgtacat ccgggtagat
     3841 tttgcgtaaa tttaccctgt cacttctaaa aggtctgtta attatgacat atatcacaat
     3901 tgaatttgat ctttgtcaat cagactgacc gtacttatca atccattgtg attgaaatat
     3961 caacgccccg gcgtgctgac caatcatcag tcttgttacc tttcatcgga ataaaacgaa
     4021 acataaacct atatgaaaaa aagtcttaaa tttgccgcgc ttgttgtttc agcaggtctt
     4081 ataatggcct cctgcgaagg gccgatgggt ccggttggtg aagatggtcc caagggcgac
     4141 aagggtgatc ccggaacaac tctgggatgt gccgaatgcc acaatcccgg cagcccgatg
     4201 tcagctttct ccgcacagtg gagtgagtcg actcacgcaa aggggggcaa tgcagcatat
     4261 gccaacaggg ccggctgcgt acaatgtcac accagccagg gattcctcga gtatctggcg
     4321 gaaggatcaa cggccaacat agctgtaccc gataatccga tgcagatcaa ctgcgccaca
     4381 tgccacaaga ttcacgagac ctactcagca gctgactggt ccctgaccaa accgggtgca
     4441 cagacactgg aggtaaagta cgccggcgct gatgtcatct gggacaaagg gagcagcaac
     4501 cagtgtgctt tctgtcacca gtcacgcact gtctctcctg cgcccgcagt caacggagct
     4561 gacttcaaca taaccaatac ccgcatggga cctcaccacg gtccgaatac caatctcatc
     4621 ctcggcaaga ctccattcga gctccctgga gaagctcatc cgaccaccaa tcctcactca
     4681 actgccaacg gatgcgttac ctgccacatg tcagccccct acggctatct tgccggcggg
     4741 cacaacatgg gagtatggta tgacagccac gggacagaga cattgcttaa gaccggatgc
     4801 acaacatgcc acaccaatac caccaccctt gataccaaaa tagctgacct taaatctgat
     4861 ataacagaaa agatggcaag tcttgaagca cagctgattg cagcaggcat ttacaactcc
     4921 gcgaccgggc ttgccaaaac cggcacattc aaggcaaatg cggttcttgc atacctgaac
     4981 tacaacactg tcaaggaaga ccgcagcaac ggtattcaca accccgggta catcagtaca
     5041 ttgcttgata actcaattgc aacaatggag gccctgggtt acccggttcc cggaaaatag
     5101 ggattgcaaa agcacaaaaa aaagctgccc gccaacgcgg acagcttttt ttttgactcc
     5161 tgatatctac tttttaagtg ttctcatata gttgacgata ctccagatct cattatcagg
     5221 aatcttcttg tcatatgccg gcatctcgcc tctgcccatc tttgtctggt aaaacatctc
     5281 tccatcagtg aatccctgga aggcggcgcc tgagaagtcg cccgggaatg ttttgcacat
     5341 tcttcccttc gggccgtcac ccaggcctgt cttgccatgg catgaagcgc agttcttgtt
     5401 ataggaaacc atgccagcct tgactgacgc atcatccttg gcgacgggat tggccatctt
     5461 cttgtactct gcagggatta cccacggttc tcccttcttc tgctgggcat ttgccgttac
     5521 tgaagcaaag gcgaacaggg ctgttgccag cacaacggaa atagttttaa atgttctctt
     5581 cataaacctt gattttaatt agactcatat tattaatgac tctcctgcca gccggtgatg
     5641 atactcctga aattactaag gggtgattta cccattgtgc tgacatagag gtgaataaac
     5701 aggaaaatta caaccaggaa ccctaggatt acatggaact ggtctgtgag gaacacgccg
     5761 ctgaatccca ggaacctctt gagtatgaac tccgggaaca tcagcgccca tcccgtgaca
     5821 aacagcatcg ggaggacaaa atacatcact cccacatatg agacctgctg caacggattg
     5881 aacttgcggt tgccggtgac gggaaaagga gactcttctc ctttaaaata accgaatcca
     5941 taatagcggg cctgcttcca gagcctttca gccatacccc tgatggcaat caggtagtgt
     6001 ctgccgtttt cggtaaagat atttccgata acaaaagccg tataactgat cgtaaggagc
     6061 acgccgcaca cattatgaat cgagactgcc cgctggaatt tcacaaccag ggcacggtca
     6121 ggatcggaat aaagcatgct gaatcctgtc acaatcagga acaggcacag gatggcgttt
     6181 gttaggtgcc ataacctgac ccagaccgga taatggtaaa aattcttact catgaactat
     6241 cttcttccgt ttgataatga tcctgaaaaa tgtgtgcacc atggctattg caattgtcat
     6301 gccaataagg aagagacctg tgaaggatat gaatttgctt cggttggctc caatcacata
     6361 cgattcattg cgcaggatca ccccgttgag aaagcccttt tcaacacggg tttcctgggc
     6421 gacatattta taaagtgtac ccatcaggat tgagttctgg ctgtgacatt cggcacaacc
     6481 ctgaacagca ctgtcagccg gcataacctc atgagccacg aggacactgt cattcatcct
     6541 ggtgtggcag tcaatgcacc tgacagcctc catgtgacga tcgacatcgg gcagccattc
     6601 atgcttcggc agcacgatac tgacattctc gtcattaatc agacccatca catcagagtt
     6661 accatggcac cggagacaca tctcatttga gcggatgata tattcccggc ctcccgtggt
     6721 ttgcctggca agaggattgt aatagtgagg gtcatgacat ttccagcatg ttatcagccc
     6781 ttctgtggca gtataatgga tgctgcgctg gtactcggca tcgatctcct caaacctgta
     6841 ctgggcatag ttcgggtcgt agccatgaca atcaatgcac ccccaggaac tctcaaaccg
     6901 cgtttcaagc ggatggggaa atgtcttgaa cccctcagca tgacaatcga ggcagttaaa
     6961 gctccagtgc acagaattgt agaacatgtc gggtacaacc aggttatgtt cagacatgag
     7021 ctgtttcctg gtggttcccg tggtggtgtc ggtaagagaa taaacaagtc tgccgtggca
     7081 taccaggcag ttgttctcat tgtcttctgc gtaaaggctg tttgaggctc cgcggaccga
     7141 ctttaccgta tcagtctcct ggccggccgc atcagggctt gcaaggagag gcagaaaata
     7201 aagtaagagc agcagctttc ttatgatcat aatccttctt ctgtcagaac cgtactgtca
     7261 cattcagtcc tatcaggaac ttaccgtcac tgatactgtt ctggttgtat acaaagttat
     7321 actgtccgag gatatctttg taatttgcaa agaaaatctg gaatgcatgt gtagctgtcc
     7381 ctatctccca tcccagtgcc agctgtggtt tggcattcag agactcatcc ttctggctgc
     7441 tgaagagctg gtcatactcg gctattatgc tgtgggagcc ggtcacattg taccttgcac
     7501 cggccgagat gccgtaatgg gcgttcttat aacccacctc aacggcattg agccagataa
     7561 atgtgggcgc aagctgcaac gaaagcttcg ttccgaactg gcgcgccacc aggatctggt
     7621 tgaaatatga aagacggtgg atttcacgcc agtcatgagc cggaccaaat gtattctgat
     7681 tcaacaggtt aatccccata ttaccgtagt agctcagtga tacgggaacc gaacctgacg
     7741 aagtctgctg cagtatggca aacttcaccg ccagatcctg tgccttgtag ttcttggcag
     7801 tacctatgcc gatcatcagt ctgtcagtaa tgccgtaatt gagccccagg cggatgttgg
     7861 caggggccca caggccgaag aggtcatcaa gggcattgtt caccgtcccg aaacggtgat
     7921 gaatttcaaa ctcgagcatg cccttgaaag gcgttgccac agtctgatta tcaattaaaa
     7981 tactggtctc aaaaaccctt accgggtaat cttcgctatc ctggcagaac accgggatag
     8041 taatcagggc cagaatgacg gatatgacaa tttttttcat ggttgacttt attaaggttt
     8101 cctggaatct aatttttttc tgctccctgt gtagcccaca gttttatgac atccgttgcc
     8161 ggaatcagaa gcgtgtcaat ctgcagcatc tgcgatccga acgctgttat cgaagaatga
     8221 atagacagta ccctgcttga agctggtgtt actgtatcaa catttgccga gagcttgtca
     8281 taagtccggt caacggtcca cgctccgtgg cagccctggc agaatggctg aagtgtttcg
     8341 gagaagctca ccgtcagatc ctggggtact tcctgcttag cccaggaata ctgttcacac
     8401 gaggaaaggg aaatcgcgcc cgccacaacc agggcagccg caaacgctct tgtaatccat
     8461 gcctttcgct tcattttgct ttctgttata ttattcacaa atataccgga ataagaatac
     8521 acccctcccg cagtcagggc atatttggat tgatatacag gtgtttcttg tcaacccgga
     8581 aattgatatt tgtcatccgg gaaactgtta aatgctttcc cgcacaggaa aagatttacc
     8641 gctaaaattg atgatcaacc ctggaaacct tatcaggaaa ggtcatcagg gagatgaagg
     8701 catgggtgtt tcagcttacc ttatagaggc caagtgcttt ttcctcgaaa gagccgatat
     8761 actctgacct cacgcggttt tccgagacag cgaatgaaat gaacaactcg gccgaggagg
     8821 tatagccggc atcacgctgg gcatcatgga gcagcaggta acccatgaca atgttggcag
     8881 ccatctcaac aaggcggcgt gcatggaaat ctgtcagttc attgttgcct gcagaaacca
     8941 cctgtgccac tctcatttca taatcaccgg ccagggagac aagttctttc ttcaggtgat
     9001 caagttccgg cctgagctcc ttcttttcat attcacgtat ctgagccagg tatcctcccg
     9061 ttgtcacgcc ccttatggca gcaacgacct gcaactggga tgtgccttca taaatggatg
     9121 tgatacgtgc gtcacggtaa agcctctcaa ccgggaaatc ctttatgaag cctgcaccgc
     9181 cgtggatctg aacagcgtca tacgctatcc ggttgcacca ctcgctgctg atcagcttca
     9241 ccatcggggt gtagatgtct gccagcctgg tgttgttctt cagatcctca cgttcctctt
     9301 ttgtaagcgg gcgttctgcc gcaataaatg accatgcctt gtatgtgtcg acatatcggg
     9361 cagtctcata gaggagtgaa cgcatcgcct caaggcggac cctcatcagg gtcagcatct
     9421 cgtaaacagc gggaaactgg tctatcgtct ttccgaactg tttcctctca gatgcatatt
     9481 ttctggcttc ctcgtaggca gcctgggcta ttccgactga ctgtgcacct actcccaggc
     9541 gggcaccgtt catcagtgac ataacatact tgataaggcc catccggcgc tcacctacca
     9601 gttttgccgg tgcattgcgg aagaccagtt cgcaggttgg tgagcccttg atgcccatct
     9661 tgttctcgat acgtctgacg atgacaccgc cgtcggccct gtcatataca aagagtgaca
     9721 gtcccctgcc atcagaggtc ccctcttcgg agcgggcaag gacgagagag atttcggcat
     9781 cgccgttggt gatgaaacgt ttcaccccgt taagcagcca tgtaccccct gattcgtcat
     9841 agtgagcctt gagctgcact gcctgcaggt cagacccggc atccggctcg gtaaggtcca
     9901 tggcggctgt agcgccttta ttgaacctgg gaaggaattc ctgccttatc tcgtcagtgg
     9961 caaactcgag gatagtctca ccgcagtcct gcagtcccca gatgtttgca aagcctgcat
    10021 cagcccttga aaccagttca gcagccatga catatggcac catcgggaaa ttcagtccgt
    10081 catactgcct cgggaaattc atccctatca ccccggccct ggtgagtgcc tcatggttct
    10141 cacgtgtgcc ccgggcatag gtaacatgac cgtcagccac agtggggccc tcatgatcaa
    10201 catcctccgc attgggagca atgacctcgg cacagatggc gccgatgatc tccagaaccc
    10261 tgtcatagtt gtccatggca tcctcgaagt catgaggggc atagtcatat ttctccctct
    10321 ccctgtaatc catctccttc agcctgacga tcttctccat cgccgggtgc ttcaggtgga
    10381 acctgaggtc cttgttgtca gtatagaaat ttgccattgc tgtttctttt tgcgttcaga
    10441 ctgctgttga ttttacctgg cgttggcctt gtagtatttg atgagcttcg gaatgacctc
    10501 tgccacatca ccgtttatga catagtcagc tatactgttg atgggggcca tcgggtcatt
    10561 gttgatggcg atgatcttgc cgctctggtt catcccggcg gtatgctgta tctgacctga
    10621 gatgccgcat gctatataaa gctttggcct tactgtcacc ccggtctggc ctacctggcg
    10681 ttcatgcccg gcgtaccctg catcgacggc agcccttgaa gcagccacct cgccgccaag
    10741 cacggaagca agctcgtaaa gcagggcaaa attctccttt gatcccatcc cgtaaccgcc
    10801 ggaaacaatt atctgggccg acttgatatc cacctttctg gcctccatgt gcctttcaat
    10861 gatctccacg gcaaggtctt cgggtctgat gaaggaggcg acatcgattt tcttaagctc
    10921 gcctctgtgg ttcctgttta cgatctcgcg cttcatgacc ccctcgcgta cggtggccat
    10981 ctgcggccgg tggtcaggat tgatgatggt ggcaatgatg ttgccgccga aggcaggtct
    11041 gatctggtag agcaggtttt tgtacgtggt atcggtcttc ttgacatagt ggtcgcctat
    11101 ctcaaggctg gtgcagtcgg ccgtcagtcc gctgtgcagg gctgatgaga cccttgggcc
    11161 gaggtcacgg ccggtggatg tggctcccat cagtgcaacc ccgggttgtt cctgcctgaa
    11221 aaggccggtg atcaccgagg tgtaaggcag ggtgcggtag tgttccagca ccggatcatc
    11281 agccacatgt accacatcgg caccataggg gaaaagctcg ttttcaatgc cgtcaagtcc
    11341 gctgccgagg accaccgctt cgaggcggca gccaagggta ttggccagtg acctgcccct
    11401 ggtgagcaat tcgaggctga cgtcggcagg cctgccttca tcaagctcgc aaaagacgaa
    11461 tagattgttc actgcaacta tattttatcc gtttatcaac caattgtatg attctcaagc
    11521 aactccctga ccagtgactc aacgtctgcg tttgacgagg taaggcgctt cgactccttt
    11581 gcctggagga ttacgttctc gatttccttc acctttgtgg gtgacccgga gagtccgagc
    11641 tgttcgggat cagccccgat ggcgtctacg ccccattcgg ttatcctgac ggatgacctg
    11701 cctgcagcaa tgtagtcaga gctttcagcc tgcagttcgg tgtcagtgcg ggcatacttg
    11761 tacttcatca ccagcctggc gttgcggtaa cggcatgccg gggctgttcc gttcactgta
    11821 actaccagcg gcagaggggc tgatactgtc tccactcccc tctcaagacg gcggcgaatg
    11881 atcagttttt cgccctcgaa atgcatctct tcggcatagg tcacctgcgg ccatcccagc
    11941 ttctcagcca cctgcggacc cacctgggcg gtatcgccgt caatggcctg ccgccctcct
    12001 atgaccaggt ccggcgccag cctgtgcact gccagcgcaa gagcatatga ggtggccagc
    12061 gtgtctgaac ccgcaaaggc cctgtcagtt agcagcaccc cgtcatctgc accacggtac
    12121 aatccctccc ggattatttc cgccgccctg accggaccca ttgtgagcag ggtcaccgtt
    12181 accccttcat aacgctcctt tattcgcaga gcctgctcca gggcattcag gtcctcggga
    12241 ttgaatatgg caggcagaac gctcctgttg accgtcccgt cagctttcat ggcatcagga
    12301 ccgacatttc gcgtatccgg aacctgcttg gcaagtacaa cgatcttgat tccgttcatc
    12361 tcattctcgc ttttgaagtg gcaaatataa caataaaacc ataccgcaaa acggatgaaa
    12421 ctgaatttca tttatgccgg tcgaaagata tatgatgtgc attatgctgt atattatatt
    12481 attatgatat tttcgccgac cggtcaatta acacgttcga aacctgaaac ttctttgtgt
    12541 cgcgctctgc cacgggtatt gaaaaatcag ttaatttcgt cgccgacaaa atcacttatt
    12601 ttttttcctc tccgatgaaa ggtagaccct acgcagccct tgttctttcc atgattttct
    12661 ggtcgttctc atttgtctgg ttcaaggtgg ccaacgttga ctatgacccc ataaccatcg
    12721 tcttcatcag gctgtgcatt gcaatactct ttctcacagg ctttctgtgg atcaccagac
    12781 ggttcaccgc cataaaaaag ggggaccgga agtatttctt cctccttgcg ctttttgagc
    12841 ctttcctgta cttcctgggt gaaagcttcg gtctgacata cgtctcatca acagtgggtt
    12901 cggtaataat atcaaccata ccggtctttg cagtcatctt cgcatgggtc atctatcgtg
    12961 agcggctgaa actgattaac tatatgggtg ttattctctc attcatggga gttctcattt
    13021 tcatcaccaa cagtacaggc aatataatga tgaacccgaa agggctggcg ctgatgttcc
    13081 tggcagtggt gtctgccgtc ggttacaaca tggttcttca ccggctggct catgaatatg
    13141 atccggtcac gatagtcaac attcagaata taattggcgc aatacttttc cttccgctct
    13201 tccttgtgtt cgatcttaag gacctgcttg ccacaggcat cgttgcgaag agcttcggct
    13261 cgataattct cctggccatc ttcgcctcgt gcggagcatt cgtgctcttt gcctattcag
    13321 tcaggtatgt cgggattgcc cgggccaaca tcttcagcaa tcttattccg gtgttcacag
    13381 caatctttgc gttcttcatc gttggcgaca ggctgacggc ccgaaacggg gtggggatgg
    13441 cggtggtgat tgccggcctc ttcctctcgc aggccaggaa gaacaggaag gcagtggttg
    13501 ataccgactt tgcagggagg agtgcctgat aaccctgaaa taccagcttt gatcaaccca
    13561 ttttgccggg attgttatct ttgtccttaa tgattcagag gatgaggcac agggctccca
    13621 atctgctttc cggcattgct gttctgcgac ggtgggctgt cgttctcctg tttctcccgg
    13681 ccctgtccgg gcaggtgctt aaaggccaga ccggcggaga caatacctat gagtttctta
    13741 acctcagtca ttcagcattt gccaatgcca ccggcggaat taacgttgcc tcgcggcgaa
    13801 gcgacctgac cctgccgtac ttcaaccccg cgctgctcga tgcctctatg gacaatgaca
    13861 tctcactaag cttctcatcc tggtttgccg gaatcaggta tggctacgcc tcctgggctc
    13921 acagcactgc cactgcaggt aacttcgccg gggggataag ttttataagc tacggcacct
    13981 tcaccgaagc cgacgaagcg ggaaatatta ccggtagctt cactgctgcc gaatacgggt
    14041 tcaatatcat ctggtcatgg cagatagact cctccttctc cttaggtgtg aatctcaaac
    14101 ctgtgatctc gaacctcgaa aggtattttt ctgcaggtct ctgtgccgat atcggggcta
    14161 cataccgcaa tgagcggatg ctgctcgatg ccgggctggt gatccgcaac gccggactgc
    14221 tgctcgtggg ttataccgct gacggaggtg agccgcttcc gtttgagatc attgccggag
    14281 ccaccaagag gctcgcccat gcccccttca gtttctccct cacactcagg cacctggaga
    14341 aacctgacct gacttacgat tatgatcctg aagatgatgc cggtcctggc ggtctcggtg
    14401 aggacattct caggcacatt gtcctgggcg tcgaggttct tccttcagac gcattctgga
    14461 tcggcgcagg ccttgactac cggcgcaggg ctgaaatgcg gaccgggcag cggaccggca
    14521 tggcaggctt tacggctgga ttcggaatac gaacaaaggc ctttgaactg gcatttgcac
    14581 atggcgcttt ccaccctgcc ggcggatcaa accacataac ggtcacactg agacctgaaa
    14641 ttatattcaa aaaaacagaa catgctgaac cttaactgca ccggttcgtt ttaacctgaa
    14701 gaagacaatg acaaggaaaa aaagaaaaat aatcattacg gttgacggat actcatcgtg
    14761 cggtaaaagc actttcgcga aagcaatagc cagggaactg ggatacatct atatcgactc
    14821 gggagctatg tacagggctg tgactctctt cgcgctgagg cgcggactgg caggtaacgg
    14881 aattgctgac gaagccggaa ttctgaatgc tcttccggag atcaccatca ctttcagacc
    14941 tgacagcgag acaggattct cagccactta cctcaatggc gagaaggtgg agagggagat
    15001 acgtgaccgc gaggtctcgg ataatgtcag tcctgtcagc actattgccg gcgtgagggc
    15061 aaggctggtg gagctccaga ggtcactggg cgccggcggg ggtgtggtga tggacggccg
    15121 tgacatagga acagtggtct tcccggccgc ccggctcaag atcttcatga cggcaaggcc
    15181 tgaggtgagg gcgcagaggc gctacctcga gctggtgagc aagggactgc cggctgactt
    15241 tgatgcggtg atgaaaaaca taagcgaacg tgacagaata gactcatcga gggaagtgag
    15301 cccgctccgt caggctgatg atgcggttgt gcttgacaac agcgacatga cgcccgaaga
    15361 gcagatggtc tggttcaggg agctttacaa aaaaaggata aatgaaagtt gacatagaac
    15421 agagttcggg attctgtttc ggagttgaaa atgccgttgc aatcgctgag gatgcactgc
    15481 gcaacggaga gaccgtgtat tgcctcggtg agatagttca caatgacatc gaggtatcca
    15541 ggctgaccgg cctcggactg aaggcaatca catatgagga gttcgcaaac ctgcgcgact
    15601 gcaaggtgct gatcagggca cacggcgagc cgccatcaac atacgcgaag gcaagggaga
    15661 ataacatcac tattattgac gccacctgcc ctatagtcag gtcaatgcag gagaagatac
    15721 gaaaggtggc cgagctgtcg cgcgacgaga ggggacagat agtcatctac ggtaaggaag
    15781 gtcatgccga ggtcataggg ctgatgggtg ccgcaggacc aaggggtata cttgtgaccg
    15841 gcaagggaga cctgcacagg atagactaca ccaggccggt cttcctcttc tcacagacca
    15901 ccaagagcaa ggtgcagtac gaggttgtgg ctgagatcat caggctcaat attgaaaagg
    15961 tctcacctga tcattcgtcc tccaacctga aggtgcacaa caccatctgc aggcaggtgt
    16021 cagggcgtga gccacgtctg cgggaattcg caatgaagca ccatgtgatc atcttcgtca
    16081 gcggaaggaa cagctccaac ggccgtatgc tcttcgaggt atgtcttaag gtgaatcccc
    16141 gctcccactt tatctcctca ccggatgagc tgaatcccga atggttcagg ggtgcggcaa
    16201 gcgcaggtgt atgcggtgcc acttccaccc cgaggtggct catacaggaa gttgcagaac
    16261 ttgttgctgc gatgtgatat tgttattttc taaattgctg aaacatgaaa ttgtagtaat
    16321 ttcgcaccgt caataaataa tttctataat ggggaaaatt aagaagatag gcgtactcac
    16381 atcgggtgga gacgctccag gaatgaatgc agctatccgc gccgtgacgc gcactgctat
    16441 ttataacggg cttgaggtag tcggcataag gcacggctac atgggaatga tcaatgccaa
    16501 tttcaagcct ctttacgcac acacggtttc agataccatc cagcgcgggg gtacaatact
    16561 caaaacagcc aggtgcgacg cgttccgtac tcccgagggg aggaaggttg cctatgacaa
    16621 cctgagagtt gcaggaattg acggtgttgt tgtgatagga ggcgacggct cattcaaggg
    16681 agccagcctt ttcaacgaag agcacaacat cccgtttgtg ggtgtgcccg gcacgataga
    16741 caatgatatc tatggtactg actacacaat aggctatgac accgcactca acaccgtggt
    16801 ggaggccgtt gacaagatac gtgataccgc cagcgctctc aacaggatgt tttttgtaga
    16861 ggtgatgggc gccgaggcgg gccacattgc cctttacagc ggaatcgcca gcggcgccga
    16921 agctatcatc atgcctgaga taaagggtga ggcgcgtgac atcaacaaga tgatcgagac
    16981 gggcaacaag cgaaagaaat caagcaatat catcattgtt gctgaaggcg atgaagaggg
    17041 aggtgccata gcgctggccg agagactcaa acctcagctc aagggctacg aggtaagggt
    17101 ggccatactc gggcacctgc agcgcggcgg ttcgccgtca gcacttgacc gcgtaaacgc
    17161 cagcaggctg ggcaatgcag cagtggaggc gctccttgac ggtcagcaga gtgtgatggt
    17221 gggactgcag aatgacgaaa tagtgctcgt tcctttcagg aaagccgtaa aacagcataa
    17281 gggactgaac cagcaccttg ttgacataat tgatatactt aacgtctgac aaagaacgtt
    17341 ttataattga tgggaccttc tgaggtatat tttacagata tgcacagcac ctcatcgctg
    17401 aacctgctga agaagctgga gaggctggtg aaagctgccg gctttgaaaa acttgatttc
    17461 gagcgtaaga tgaccgctat caaggtccat ttcggtgagc cgggcaacct cgcatatctc
    17521 agacccaact ttgcagcaag gctggcttcc atggtgaagg agaagggcgg taaaccgtac
    17581 cttaccgact gcaacacact ttattcaggg gagaggacaa atgcacccga ccaccttgat
    17641 gctgcattct caaacgggta caaccccctc agtgcagggt gccatgtgat aattggcgac
    17701 ggagtcaagg ggactgatta ccgtgagata gaggttaacc tcgattatac gcagacggcg
    17761 atgatcggta cggcgatagc cgatgctgat ataattatca ccctgaatca cttcaagggg
    17821 catgagcaga ccggtttcgg aggtgcattg aagaacctcg ggatgggctg tgcttccatt
    17881 ggaggaaagc ttttccagca ctcggggaat ccgcccttta tttaccagaa gaactgtacc
    17941 ggctgcaaca tctgcgtgat cagttgtgca catgatgctg ttcacctggg tgaagaccgt
    18001 aaggcattta ttgacaacga caaatgcacc ggctgcggcc agtgtgtggc tgtctgccag
    18061 tttgatgccg ccagggtgca gtggagctct gagggagacg tgctcagcaa gaaagtggca
    18121 gagtatgctt ttgccgttgt gaaagataag cctgcactgc atatcaactt catcattgat
    18181 gtttcgcccg actgtgactg ctgggggcat aatgacaggc cactggttga ggacatagga
    18241 attgcagcgt ccacagaccc gcttgccctt gacaaggcat gcgccgatat ggtcatggaa
    18301 gcgcctgcgg catggcattt gagggaaaag gataatgaca atgatctccg cggggaggat
    18361 aagttccgga tagcgcatgg cagcaccgac tggaacacgg ggctccttca cggggagaag
    18421 attaaacttg gatctttatc atacaaactg attaagatat gaaggacccg tcatcatcaa
    18481 agcgaatggc cattctcatc gttgaggatg atccgcatag tcagctttat ttcacgaagc
    18541 tgctgtcagg tatgtatgac actgctgtgg ctgagtccgc tgcagaggca tgggatcagc
    18601 ttcatgaccg gaagtttgac ctggtgctga tggatatctc ccttaagggg gatgaggatg
    18661 gtctctcact gacccgcagg ctgcgtgtcg aggatggtta tggaactctg cctattgtag
    18721 cggtgacggc atatgctttc cctgatgacc ggaagcgggc gatggaagcc ggctgcactg
    18781 attatctgcc caagccggtg agcagcaacg acttgcatcg caagatcgcg gagctgactc
    18841 ttaaatagtc aaaaagttcc ttcaggataa aaattttatt at
//
LOCUS       DAOPXU010000052        18839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_3063_length_18839_cov_11.756495, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000052 DAOPXU010000000
VERSION     DAOPXU010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18839
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3063_length_18839_cov_11.756495"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(314..742)
                     /locus_tag="PKK03_06840"
     CDS             complement(314..742)
                     /locus_tag="PKK03_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84155.1"
                     /translation="MKTRLFFTVAAFVAMTVVAGAQTTDITPGQTGKGRAAGNTFVDA
                     DKDGVCDNFENGTRPGHRAYSAGENQAAANRGPGKGQGLAQGNRKGQGRSTGMAAGRG
                     AGRAQGTVPGRGRNSGHGPAFVDADKDGVCDILEATAEKK"
     gene            complement(856..3072)
                     /locus_tag="PKK03_06845"
     CDS             complement(856..3072)
                     /locus_tag="PKK03_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNX84156.1"
                     /translation="MTEKSFAIEGMNCASCAAHVEKAAAKVEGITFASVNLATERLTI
                     SYDERKASLSQVVDAVNRAGYKAIDDEKERRAARPEAERLRKRLIWSLVFTVPLLIIT
                     MSSMAGARLPDFISPATSPLNFALLQLLLTVPVVITGRSFYIAGTKTLLRGNPGMDTL
                     VAMGTAAAFLYGIYATIRIAGGDSHYVHSLYYESAATIITLITMGRFLEARSKARAGN
                     AIRQLLSLAPPLATVIRDGKEKTVPASKVQVGELVVARPGERFAVDGTVSSGTTSADE
                     SMLTGESTPVEKEEGSEVTGGSINLNGSVVYRATRVGSETTLARIVKLMEEAQGSKAP
                     IARLADKISGIFVPVVMALALLAGTAWLIGTGDFRLAFTVLISVLIIACPCALGLATP
                     VAVMVGTGRGAQQGILIKGGEALETARKAKVVVLDKTGTVTTGVPVVTDLVPADGTDE
                     STLLTLAATAESRSEHPVARAVISLAGDKGFAISAPERFSALPGYGVEASVNGIHILT
                     GSRRLMNERKIDTSASDQAADELSDQGKTVMYIASGGKCLGIIAVADTIRSGSAAAVG
                     EMKAMGLGVMMITGDSIQTARHIAGEAGIDDVIAGVLPGDKAGEIRKIKSTGKSVIMV
                     GDGINDAPALATADVGIAIGSGTDVAIEAADVVLIKNDLGDVVRTLKLSHSTIRNIKQ
                     NLFWAFFYNILGIPVAMGLLHLFGGPLLNPMIAAAAMSFSSVSVVWNALRLRNIRL"
     gene            complement(3159..3395)
                     /locus_tag="PKK03_06850"
     CDS             complement(3159..3395)
                     /locus_tag="PKK03_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84157.1"
                     /translation="MKKDVPGEKNAALSGTFVSRTFDGGYNAFPTFVREMDQYLSESG
                     KKAKDYYVHYASCPKCAKKFGHNYMILFTEVSNN"
     gene            4457..5953
                     /locus_tag="PKK03_06855"
     CDS             4457..5953
                     /locus_tag="PKK03_06855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNX84158.1"
                     /translation="MADFLKKLLKNELVLFWIVFAVAIVFYSINIGLSDLWSDETYTK
                     SMLYGSLSDLYAKFKTDLHPPLYYLGLRLFTGIFGLSAVSLRIFSVVGVLATLLLGYF
                     AGQRIFGKPGALYLCLMLVSVPMVAVYSHQARMYSWAAFSITGVFLYSCLFLRTGTTR
                     DLILILVFTVAAMYTHYYSLIAACMANMFVLIYLVLTKNRKWINHLIVMLIAAILFLP
                     WLSMFVVQVKRVQSAFWAPEVSLSTIGSCFAIPFTEQFWTTPSSKSLTILMYTLMVLT
                     IILSFKKSFSGYRIPLWLSLCIFIGTLIIAMIISLFSQPILYSRYVAVIVTMLIIPAT
                     ILLTVIKTKWVRIISIPVILFFGIRISVSAFAFSYGPYKQTIEYITKTYPEIDKILHI
                     TEVTAGPMVEYNGNSGLKHYWLKAEMSNVDAFTQVKQYQKPEEFLSPGEDFCAVQFHN
                     NPLNQNNLDLVLAESELIKKDTVFDAKFQNGIFIQLYLLKYKGSKPAQ"
     gene            6205..6597
                     /locus_tag="PKK03_06860"
     CDS             6205..6597
                     /locus_tag="PKK03_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNX84159.1"
                     /translation="MKRKLIILSAGLLILLISGFLALYIWIDIDVKKNINHARGIYPG
                     TAEDALIAYLLDTNNSPEDRSHIAIWTLGQIHSQKALPILKGLYNNDPEGKTCYGKHD
                     SVLCQYELYKAIKAEESNWWPLHSRLNK"
     gene            6983..7411
                     /locus_tag="PKK03_06865"
     CDS             6983..7411
                     /locus_tag="PKK03_06865"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNX84160.1"
                     /translation="MKDNILNLTPRQISLKWIDAYNSHNPDTATALYDENVTNTQLPY
                     GKAVHGREAMKKTYENLFRAFPDIHIEAENIIEQDSWVVVEWIFQGTMKGEFAGHSPN
                     ENKFRMHGCEIFQVVNGKIFAQHGYWDKNSMFSQLKLNID"
     gene            7640..8416
                     /locus_tag="PKK03_06870"
     CDS             7640..8416
                     /locus_tag="PKK03_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84161.1"
                     /translation="MKNAYKKAVWEALERTMNIDNETLTTADIKHLPAIIQKYLHFTG
                     SVGKEKVSNFRAEFVGGIRSKSTEDFMPLKSVQYNFTDKPTRLFYIVAKKKGIPAKGI
                     HLYKDQTAIMLIKIFGLFTVVNAKGKEMNQGETVTLFNDMCFMAPASLIDRNIEWEEI
                     DNITVDAKFTNDNITISARLFFTEKGELVNFSSNDRFETSDGKTYKNFPWITPVTGYT
                     NINGYCLPSGAKLIYRHPDGEFCYGEFDLKSIEYNCKVFR"
     gene            8542..9291
                     /locus_tag="PKK03_06875"
     CDS             8542..9291
                     /locus_tag="PKK03_06875"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNX84162.1"
                     /translation="MKSRLILLTFLICDTMVSQITDPGMVLIPAGEFTMGKNSAGRTD
                     WQPEHIVTVDAFYMDKCEVTNKQYYEFCKMTGNPLPEFWGLSQFKCTLEYPDHPVVGV
                     SFADAMKYASWAGKRLPTEAEWEYAARGGLPDSNYPWGNQIDSTFTNYGKKYKTTLKA
                     GSFKPNGYGLFDMGGNVWEWTMDNYGSDYYLNSPSQNPSGPDSGRFKVIRGGSWHSGA
                     MCIQTYFRNGLPASWVDFAVGFRCVMNAIQP"
     gene            complement(9399..9485)
                     /locus_tag="PKK03_06880"
     tRNA            complement(9399..9485)
                     /locus_tag="PKK03_06880"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9449..9451),aa:Ser,seq:cga)
     gene            9578..9736
                     /locus_tag="PKK03_06885"
     CDS             9578..9736
                     /locus_tag="PKK03_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84163.1"
                     /translation="MDVRTIEEAKELVKGDPTVTSGIFVTELYQWYGSAAVSMLNEIH
                     QKIQKQQP"
     gene            9964..10695
                     /gene="bla"
                     /locus_tag="PKK03_06890"
     CDS             9964..10695
                     /gene="bla"
                     /locus_tag="PKK03_06890"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase"
                     /protein_id="HNX84164.1"
                     /translation="MRRTALFIIMIITHYQLQSQDTISISADLELIRISNNSFVHVSY
                     SEVPGYGRVAANGLIFIEGHKAFLFDSPWDDTLTEILVTYLEEEMGLKIKGFVPNHWH
                     DDCMGGLGYLKSRKIISYADSLTLLLARENKLHVPDHGFKDSLILRLGRKEIRCYFPG
                     AAHSTDNIVVWIPSEKILFPGCICKSADSQNLGNTTEGDLAAYPATVEWIIRKFPDVN
                     TVIPGHGAFGGPELLTHTLALTRNK"
     gene            10705..11805
                     /gene="rsgA"
                     /locus_tag="PKK03_06895"
     CDS             10705..11805
                     /gene="rsgA"
                     /locus_tag="PKK03_06895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNX84165.1"
                     /translation="MSQNNDEEPKSGIGQLGLTPEMNSYIIDNLPEGTLVARVIREHR
                     ERYAVSDGENEYDAEITGNMRYSAASRKDFPAVGDWVTITPFDNDRAIINQILPRRSV
                     LARQAVGKPGEIQIISANIDYAFIVQAISNNFNINRLERYLTICNSAGIEPLLVLSKI
                     DLVSREVTDEAVRALAKRDRHMKLVLLSNVTREGVDSVISLMERGRTYCVIGSSGVGK
                     STLINTLLQREALRTGAISSSTNKGRHVTEHRELFVLENGSIIIDTPGMKELGLTDDS
                     TGLSATFREIADVAAECRYPDCRHVSEAGCAVLEAVEKGDIDRDSYENYLKMLKEQER
                     FTTTVAEKRRKEKHFGKILKDYHRIQRKNQEE"
     gene            11910..12377
                     /locus_tag="PKK03_06900"
     CDS             11910..12377
                     /locus_tag="PKK03_06900"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNX84166.1"
                     /translation="MLLSGLLRDYDGSRQIELAAFNGEDYFAVPGQMNYIAANEGKFG
                     NMILDINIDGAGYQEGPSAFSEFNLPEELSVKLARIIDRYPGITSGPQLPQGDHSIFV
                     QFGVPAVAVTSKWFIDNMDNQDVTHTPKDRPEITDCHRLVEIANALNEFIRMI"
     gene            12465..13055
                     /locus_tag="PKK03_06905"
     CDS             12465..13055
                     /locus_tag="PKK03_06905"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNX84167.1"
                     /translation="MKTRILSFLVMMLLGSLMQTSEAQAPAAPVKPALLVIDIQNAFL
                     SMIPEADKAKAMANISYYMSVFRQHGYPVILVYHHGEEYGVKPGTELFEFPESVPIRP
                     EDPKVIKTYPDAFNKTDLDKVLKEHGCNTLFLTGLSAVGCVLATYVGSMNNDYRAFLV
                     KDAIMSHNSEYTQNIEIMFEAVGSEVVKMVLETSGK"
     gene            complement(13279..13602)
                     /locus_tag="PKK03_06910"
     CDS             complement(13279..13602)
                     /locus_tag="PKK03_06910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX84168.1"
                     /translation="MSLSTAAGQTGDGQPVQARSAVTDSVAHNLAERIKIYPNPVKSE
                     LIADHIEGITMIEIFDVTGNKCLNEVCDNHNQMIIEVSLLRRGIYFMRFISPRATIMK
                     RFIKE"
     gene            13933..16005
                     /locus_tag="PKK03_06915"
     CDS             13933..16005
                     /locus_tag="PKK03_06915"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNX84169.1"
                     /translation="MMTVVLAAVSCNTQKKEQGNPFFAEWKTPFSVPPFDQIKNEHYL
                     PAIDSGIVLARLQIAEITANAEAPTFANTVARYDRAGELMSKVAYVFYSQTSANTNDT
                     LEALQMEIAPKVSAFSDEVLLNAELFNRIKAVWENRSAEQLNDEQLFMLENMYKSFVR
                     NGALLGPEDQEKLKKLNQELSVLTVKFSQNVLEETNSFRLVIDNEADLKGLPGSVIAG
                     AADMAKSDGMDGKWVFTTQKPSMIPFLQYADNRDLRKKLYDAYLNRGNNGNENDNNKI
                     IADIVRLRAERAKLLGYRTHADIVLETRMAKTPDNVLNLLNNLLERSLPVAKRERDEM
                     QKIADVGGGNFKIEPHDWWYYAEKLRKAKYDLDENELRPYFSLDNVRDGAFAVANKLY
                     GITFTRIENIPLPHPDAQAFEVKEADGSHVGVLYMDFYPRESKRQGAWCGGYRDHKVV
                     DGREITPVVTIVGNFTPPTGGRPALLSMDDVLTLFHEFGHGLQGLFSENTYSSTTVAW
                     DIVELPSQIMEHWATKPEVLAMYAKHYETGEVIPEALVEKIRNSSFFNTGFDNVEIMA
                     ASLLDMAYYSLEAPVQVDPQQFEKGTMKKIGLIPEIEPRYRSTYFLHMIDGYDAGYYV
                     YTWAAVLDNDAFEAFNEKGLFDQATATSFRKNVLEKMGIMDAEKMYLNFRGREPEIEP
                     LLRNRGLN"
     gene            complement(16067..18292)
                     /locus_tag="PKK03_06920"
     CDS             complement(16067..18292)
                     /locus_tag="PKK03_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNX84170.1"
                     /translation="MEATVAKQKFGTAPVYFTAISTILGAILFLRFGFAVGTLGFWGT
                     LLLIVMGHAVTIPTALAISELATNKRVEGGGEYFIISRSFGLNIGATIGIALYLSQAI
                     SVAFYIIAFTESFEFLFNFVAQRFSFVLPRQVVSLPVTVILGLMILRKGSNTGVKMLY
                     AVIAILFVSLLLFFLGKPAQGSLATGQLPVRDIRNMSDFFIVFAICFPAFTGMTAGVG
                     LSGNLRNPARAIPLGTILATATGIVVYVLVIWKLAISASPEEMLENQLIMGKIAIGGT
                     VIIPLGLAASTLSSALGSVLVAPRTLQALAKDTSFSSMRLNRWLAAARNNDGEPVNAT
                     LVTLLIASAFVALGNVNAVAEIISMFFLVTYGSLCLISFLNHFGSSPSYRPSFRSKWY
                     LSLTGFVVAVIVMFRINTLYALTASVLIVIIYSYINHYHKNRTGLESLFANTLFQINR
                     NLQVYLQKSGSRRKEKEWRPGVICISKDSFRRERAFRLLNWLSYSYGFGTYLHRIDGY
                     YSRSTSQQATEELAKLIELYETSHNHVYVDTIISPSYTSAIVQAIQVPGISGMENNMV
                     VFEYDKENPENLPEIIDNIRLIKAGDFDICLLASASREMICNKGIHVWIDFNEPENAS
                     LLILLSFIISGHPDWKKSFIKVFSTCKPGQYEETRRELEGLITSGRLPITEKNVEIIE
                     VTHGSSVKSLINEKSSTAAMVMTGFRPEALRQNGETILGGYDAAGTVLFVNSQYQKEI
                     V"
     gene            18400..>18839
                     /locus_tag="PKK03_06925"
     CDS             18400..>18839
                     /locus_tag="PKK03_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNX84171.1"
                     /translation="MNQETVAGSDLKEIEEFLGGLGIDGRHSRRLLYWVYRRGIRSFE
                     EINDIPLKFLRVLSENAVPGLSLPIASEVSSDGSVKYLFTTKNGMAHETVFLPDGKRR
                     TVCVSVQSGCRMGCRFCATGKNGWNGNLTAGEIVNQVISIPHEIT"
BASE COUNT         5057 a         4323 c         4581 g         4878 t
ORIGIN      
        1 caaaaccggg catctcaatg gcgcgcagcc gttccagcgt agtggtgatg acgcccgatt
       61 cgttgtacat cgggatgatg atggtgaggt acaatgtagt tgtttacagg tttgaggtta
      121 aaggtagcac tcaaatccga gagagcaatg ggggtgacag tggtgaatgg cctacggcca
      181 attaatgcgc gtagcgcata aagcattgta gaacaggaga taatatcagg agattggtcg
      241 tagaccaata agcattgtaa aaaaagagac cccaaaatag ggatctcttt ctgtatttag
      301 ccgtgagtgc tggttacttc ttttctgcag tggcttccag gatgtcgcaa acgccgtcct
      361 tgtcggcatc gacgaatgcc ggaccatgac ctgagttccg tccccttccg ggcacggttc
      421 cctgtgcacg accggcacct ctgccggctg ccattcccgt actgcggccc tgtcccttcc
      481 tgttgccctg tgcgaggccc tgacccttgc cgggtccgcg attcgcagca gcctggtttt
      541 ctccggcaga ataggccctg tggccaggcc tggtgccgtt ctcaaaattg tcgcatacac
      601 cgtctttgtc ggcatccaca aaggtgttac ctgctgcacg gcccttacct gtctggccgg
      661 gggtgatgtc cgttgtctgc gcacctgcaa caaccgtcat ggctacgaac gccgctaccg
      721 taaagaaaag tcttgttttc atgagtaatt gtttttgtta ttgttttcta tgattgttca
      781 cagtttacaa ttactcagat gttgattacc tccataggtt taatcgcgac atcaaaaaaa
      841 cagtaaaatc atggattata atctgatatt tctgagtctc aaggcattcc agacaacaga
      901 taccgaactg aaactcattg ctgcagcggc gatcatcggg ttgagcaacg gtccgccgaa
      961 gaggtgaagc aatcccatgg ccacgggtat gccgagaatg ttgtagaaga atgcccagaa
     1021 aagattctgt ttgatattgc ggattgtact gtggctcagc ttcagtgtgc ggaccacatc
     1081 acccagatcg tttttgatca gtaccacgtc tgctgcctca attgccacgt cggttcccga
     1141 accgatggcg atcccgacat ctgctgtggc gagtgccggg gcatcattga tgccatcgcc
     1201 gaccattatg acgcttttac cggtgctttt tattttcctg atttcgcctg ctttgtctcc
     1261 cggaagcaca cccgctatca catcatctat acctgcctct ccggcgatat gcctggcagt
     1321 ctgaatgctg tcgccggtta tcatcatcac tccgaggccc atggctttca tttccccgac
     1381 tgctgccgcc gacccggatc tgatagtgtc ggctacagca ataattccca ggcatttgcc
     1441 tcctgaagca atatacatga ccgtcttacc ctggtcagag agttcatcgg cagcctggtc
     1501 tgaagcggaa gtgtcgatct ttctttcatt catgagtctc cggcttcctg tcaggatatg
     1561 aatgccgttg accgatgcct ccacaccgta accaggcaga gcgctgaagc gctcaggagc
     1621 ggagattgca aagcctttat cccctgcgag gctgatgact gccctggcaa cagggtgttc
     1681 tgaacgactt tccgctgtag cagccagagt aagaagtgta ctctcatctg tcccgtcagc
     1741 cggaaccagg tctgtcacta ccgggacgcc ggtggtgacc gttcctgttt tatccagcac
     1801 caccaccttt gctttgcgtg ccgtctcaag ggcttcacca cccttaatga ggataccctg
     1861 ttgtgctccc ctccctgttc ccaccatgac tgccaccggt gttgccaggc ccagtgcaca
     1921 ggggcaggct attatcagta ccgagatcag taccgtgaat gccagccgga aatctcccgt
     1981 acctatgagc caggctgtcc ctgccagcag ggcaagtgcc atgacgaccg ggacgaagat
     2041 gcctgatatc ttgtctgcca gccttgcaat aggggccttt gatccctggg cttcctccat
     2101 cagcttcacg atccttgcca aagtggtttc actgcccacg cgtgttgccc ggtaaaccac
     2161 cgaaccgtta aggttgatgc tccctccggt cacctctgag ccctcttcct tctctaccgg
     2221 ggtactctcc ccggtgagca tcgattcatc agctgatgtt gttccgcttg agaccgtacc
     2281 gtcaacagca aaccgttcgc cgggtcttgc caccacaagc tctccaacct gcaccttcga
     2341 ggcaggtact gttttttcct tgccgtcgcg tatcactgtg gccaggggtg gagcaaggga
     2401 aagcagttgc cggatggcat tgcctgccct ggcctttgac cttgcctcca ggaagcgacc
     2461 cattgtaatc agggtgatta ttgtagcggc tgactcatag tagagactgt gcacatagtg
     2521 tgaatcacct ccggcgatac ggattgtggc atatattcca tacaggaatg ctgccgcggt
     2581 acccattgcc acgagggtat ccattcccgg attgcccctg aggagtgtct ttgtgccggc
     2641 gatgtaaaag ctgcgtccgg tgatcacaac aggtacggta agcaacagtt gcagcagtgc
     2701 aaagttgaga gggctggtcg ctggtgagat gaagtccggc agccgggccc cggccatgga
     2761 agacattgtt attatgagta gcggaacagt aaagacgaga gaccagataa gtcttttcct
     2821 gagcctttca gcctcgggac gtgctgccct gcgttctttt tcgtcatcga tggccttgta
     2881 gccggccctg tttacagcgt cgaccacctg gctcagcgat gcctttcgct catcgtagga
     2941 gatggtcagt cgttccgtgg ccaggttgac gctggcaaat gttatgccct cgacctttgc
     3001 cgcggctttc tctacgtgtg ctgcgcagga ggcgcagttc atcccttcta tggcaaatga
     3061 tttttcggtc atcactctgt cagtgtaaaa ataaaacaat gggccggcga gaaagttgaa
     3121 atacagccgg cagacctttg acaaatgact ccggagactt aattattgct cacctctgta
     3181 aaaaggatca tgtagttatg accgaatttt ttggcacatt tcggacaaga ggcatagtgg
     3241 acataatagt cttttgcctt cttccctgat tctgacaggt attgatccat ttccctgacg
     3301 aatgtcggga aggcgttata gccaccatcg aaggtcctgc ttacaaaggt gcctgatagc
     3361 gcggcattct tctcgcccgg gacatctttc ttcacggaaa tcagaatatc agacctgaat
     3421 gccgtaggat catgaaacat aataagtgtg tcttttcatc ccatttttca gggtggaatt
     3481 caggacaaca ttcctggttt tgtgtttgtt cgttcatgtt atcagtgttt ttgttggtga
     3541 tttccccaag agacagctaa gttatgaaat ccgtcagatc aggattgaac aaaatgtgga
     3601 tttgatcaag tggcgttacc tgggatgatc cattcttaat gtatccatgc ggagtccggg
     3661 aatttagcga cggttggatt taatgctgta atccatcttt aatgagagac tgtaatattt
     3721 ttattgtcta ccttagtatg gcaatcagtc tgttggtctc tttggttaat gtgtttccgg
     3781 gtgaagtggt ggttgggtgg ttagcactgg gactgacggg gctgtgcatt aagtgacgcg
     3841 gagcagaagc aaccattttg gtcattgtct ggcgccaggc tgaggagccg gccggagaaa
     3901 tggaaagctt ctttgaaata actgattcat ggactacttc accgagtaaa gaactgatta
     3961 agaaaacatt tgccggccac aatatgattg ttgttggccc accgttaaaa tttgtaatgg
     4021 ttcttgcacc cggacacgcg catcagctta caaataaatt ccaccttacc ttgatagttt
     4081 tcgggctttg atcatgtttg aaccacgaca ctgcgccagt acccgcacca aacttcgtta
     4141 ccgggtataa attcgcgact ccgatcaaac tttgtaccgc gaccgcgcca gaactcaatc
     4201 aaatttgtac ccggacggca aaaacacgcg ccagaccctc cttgaaccgt gacagttttg
     4261 tggccacgct gccgcggggt accaggattc tacccggtgc tgtcgtcggg taacgcttac
     4321 taagccctac cgccgggccg ttatagggca tttaccgtac caatctgaca atcagtcaaa
     4381 tccataagac attgactcaa atatcttaac catttatcga taaaattatc tcattcattc
     4441 atttaaattt gattacatgg cagattttct aaaaaaacta cttaaaaatg aacttgtgtt
     4501 attctggata gtattcgctg ttgcgatagt tttctattct attaacattg gattaagcga
     4561 tctttggagt gatgaaacat acaccaagtc gatgttgtat ggttctttat ctgatcttta
     4621 tgctaaattt aaaactgatt tacacccgcc attatattat cttggattaa gattgtttac
     4681 aggcatattc ggcttatcag cagtatccct gagaatattt tcagttgttg gagtattagc
     4741 gaccctgtta ttagggtatt ttgccggtca acgtatcttt ggtaaaccag gagcattgta
     4801 cttatgtctg atgctggtat cagttccgat ggtggcggta tattcgcatc aggccaggat
     4861 gtacagctgg gcagcatttt ctataacagg ggtattttta tattcctgtc tattcttacg
     4921 aactggcacc acccgggatc tgattcttat acttgttttc actgtggcag cgatgtatac
     4981 tcattattat agcctgattg ctgcatgtat ggcgaatatg tttgttttga tttatcttgt
     5041 acttaccaag aacagaaaat ggattaatca cctgattgta atgctcatag cggccatatt
     5101 gtttctcccg tggctctcta tgtttgttgt tcaggtaaaa agagtacaaa gtgctttttg
     5161 ggctcctgag gtaagtctat caactattgg ttcttgtttc gcaataccat tcactgaaca
     5221 attctggaca accccttctt ccaaatcttt gactattttg atgtataccc tgatggtttt
     5281 aacaataatt ttaagtttta agaaatcgtt ttccgggtat agaatacctc tgtggttgtc
     5341 attgtgcatt tttattggca ctttaattat agcaatgatt atttctttgt tctcacaacc
     5401 aatactttat tccagatatg ttgcggttat agtcacaatg cttattattc cggctacaat
     5461 ccttttaacc gttattaaga caaaatgggt gagaataatt tcgataccag ttatcctgtt
     5521 ttttggtatt cggatctctg tctcagcttt cgctttttca tatggacctt ataaacaaac
     5581 catcgaatat atcaccaaaa catatcctga gattgataag attttacata ttaccgaggt
     5641 cactgctggc cccatggtag aatacaatgg gaattcggga ttaaaacatt actggcttaa
     5701 ggcagaaatg tcaaacgttg atgcttttac ccaggtaaaa caatatcaaa aacccgaaga
     5761 atttttaagt ccgggtgagg atttctgtgc agtccaattt cacaataatc cattgaatca
     5821 aaataacctg gaccttgtct tagcggaatc ggaacttatt aagaaagaca cagtgttcga
     5881 tgccaagttt caaaatggta tttttattca actttacctt ttaaagtaca aaggcagcaa
     5941 gcctgctcaa tgataaaatt cttctttctt gatttgaaaa acaaaatacc ctgactgatt
     6001 ttaaaacatg ctgattaaaa tagctcccta taacgcgggg taccaggaca gttgccgggt
     6061 actgcgtcag gtaacgctta ggaaattata ccgccgggct gtcgggtggc atggatggtc
     6121 cttcgtataa agacttatga tccgggaaac tttcacctgg agaattatag acaataaact
     6181 tcttgagaaa taataacaga aaaaatgaag agaaagttaa tcattttgag tgctggtctt
     6241 cttatcctgc taatttcagg attccttgca ttatacatct ggatagatat agatgtaaag
     6301 aaaaatatca accatgccag gggaatatat cctggtactg ctgaagatgc cttgatagct
     6361 tacttgctgg atacaaataa ctctcctgaa gatcgttcac atattgctat ctggacattg
     6421 ggtcaaattc attctcaaaa ggcattaccc attctaaaag gactttataa taatgaccct
     6481 gaggggaaaa cctgttatgg aaaacatgat tctgttctgt gtcaatatga gctatataaa
     6541 gccattaaag ctgaggaatc caattggtgg cctctgcatt ccaggttaaa taaataaatt
     6601 cttttaccac ccgacacgga caatattggc aatttgtttg tcatggtttt tgctaacgtt
     6661 tcttcccccg ggacatattt gtaatggcat aaccacgccg tcccctcttt gctccgggac
     6721 tgagatatgg cccgcttccc ggcgggtgcc cggattgtta ccggatgctg cttccggtga
     6781 cgcttacgaa gttataccgc cgggccgctg gcagcaagtt gggttccggt ccagacctgg
     6841 actttaagac aaagatttga cacggatcct aataactatt aatggatatt acacgactaa
     6901 aaagtatata attctagttt ttctgttttc attttcaatt tccatgaagc attcttattc
     6961 tcgggacaaa tcactaaata ttatgaaaga caacatttta aatctgacgc caaggcaaat
     7021 ttccctaaaa tggattgatg catataacag tcacaatcca gatacggcaa cagcccttta
     7081 tgatgaaaat gttaccaaca cacaacttcc atatgggaaa gcggtacatg gacgtgaagc
     7141 aatgaaaaag acttatgaaa atctttttcg ggctttcccg gacattcaca ttgaggctga
     7201 aaacataata gaacaggatt catgggttgt tgttgaatgg attttccagg ggacaatgaa
     7261 aggtgaattt gcgggacatt cacctaatga aaataaattc agaatgcatg gttgcgaaat
     7321 atttcaggtt gttaatggga aaatatttgc ccaacatgga tattgggata aaaactcgat
     7381 gtttagtcaa ttgaaattaa atattgatta gacttgattt ttgggaaatc ctgattgata
     7441 aaatataagg aaaaaaagat agaagaaatc atccagccgc cagtacggac tgactgataa
     7501 cggacttgtc agactttttg cttctgatcc tgccctcaag acatgtttgt gatggccgta
     7561 atacgtaata cgagacgttc catatcccaa atctccttta ggatttattc cgtaatttcg
     7621 aaccagataa cgttgtaaaa tgaaaaacgc ctataagaaa gcagtttggg aggcattaga
     7681 acgcacaatg aacattgata atgaaacact gacaacagca gatattaaac acttacctgc
     7741 gattatccaa aagtatcttc attttaccgg ttccgttgga aaagaaaaag tatcaaattt
     7801 cagagcggaa tttgtcgggg ggatcaggtc aaaatccact gaagatttta tgcctcttaa
     7861 gtctgtacaa tataatttca ctgacaaacc cactcgactt ttttacatag ttgcaaagaa
     7921 gaagggaatt cctgcaaaag gaatccactt atacaaagat cagacagcaa taatgctgat
     7981 aaagatattt ggactattta cagtggtgaa tgctaaaggt aaggaaatga atcagggaga
     8041 gactgtgaca ttgtttaacg atatgtgttt catggcaccg gcttcactga ttgatcggaa
     8101 tatcgaatgg gaagagatag acaatattac agttgatgct aaattcacca atgataatat
     8161 taccatcagt gccagattat ttttcactga aaagggcgag cttgttaact tttcaagcaa
     8221 tgacagattt gaaacttcag atgggaagac ttacaaaaac ttcccctgga ttacaccagt
     8281 gacaggttat acaaatataa atggatactg tcttccttcg ggggctaaat taatctatag
     8341 acaccctgat ggagaatttt gctatggtga gtttgacctg aaaagcatcg agtacaactg
     8401 caaggttttc agatgaggga ccaacacata aagcggacgg cagggtcaat aagcctgtca
     8461 tggtttttac aaaatcctgc tgaatcttgt ttccggtcac ccgtccctgc ccgcaattga
     8521 aatcctgcaa aaattaacaa tatgaaatca agactaattc tgctgacttt cctgatctgt
     8581 gacacaatgg tatcacagat tacagatcct ggcatggttt taatccctgc cggagaattc
     8641 acaatgggaa aaaactctgc cggaagaacg gattggcaac ctgagcatat agtcactgtc
     8701 gacgcattct acatggataa atgtgaggta accaacaaac aatactatga attctgtaaa
     8761 atgaccggca atcccttgcc cgaattttgg ggcttgtcgc aatttaaatg tacccttgaa
     8821 tatcctgatc acccggttgt gggagtcagt ttcgcagacg ccatgaagta tgcatcctgg
     8881 gccggaaaaa gactcccaac tgaagcggaa tgggaatatg cagcaagagg tggactacca
     8941 gactctaatt acccctgggg aaaccagata gactcgacat ttacaaacta tggtaaaaaa
     9001 tataaaacaa ctcttaaggc aggttccttt aaaccaaatg gatacggact atttgatatg
     9061 ggtggcaatg tatgggaatg gactatggat aattatggat ctgactatta tttaaattct
     9121 ccaagtcaaa atccaagtgg tcctgactca ggtagattca aggttatacg cggtggaagc
     9181 tggcattccg gagcaatgtg tattcaaacc tacttcagaa atggactgcc cgcatcctgg
     9241 gttgactttg cagttggatt cagatgcgta atgaatgcaa tacaaccatg accacccggg
     9301 tttttttctt acggctcccg ggcttataaa agcaagcttt caacaccctg tcaccgtaat
     9361 aaaaaacccc gcctggcgcg gggttcatta aagctttgcg gagagagagg gattcgaacc
     9421 ctcggtccag ttgcccggac aacggttttc gagaccgccc caatcgacca ctctggcatc
     9481 tctccggact cgctgtgccc gcctggcacg gcggatgcaa atataactca aattcttaag
     9541 actcattttt gatcgtcaaa aacttaaaat aattctgttg gatgtcagaa ccatagagga
     9601 agcgaaggaa ctggtgaagg gtgaccctac agtaaccagc gggatattcg tgaccgaatt
     9661 ataccagtgg tacggctcgg cagcagtatc aatgctgaat gagatccatc aaaagattca
     9721 gaaacaacaa ccctgatatc gtttcatact gtaaaacagt attatacatc aaccatccgg
     9781 acgatatgaa aaatttttcc ggtaaagtgt aaacttttcc ggattttctc cgtctttatg
     9841 taaagagatg tgaaaatctc ctttggttgg tttcaggtaa acacaaaaaa gccgcatcat
     9901 cagcggcttt tttcttaaat tcgatatctg taatacagcg taccatagtc aataaatccg
     9961 gccatgagaa ggacagcact tttcatcatc atgatcataa cacattatca gcttcagtca
    10021 caggacacaa taagcatttc cgccgatctt gaactgatac ggatctcgaa taattccttc
    10081 gtgcatgtgt cgtacagcga ggtgcccggt tacggcaggg tggcagcaaa cggactgata
    10141 ttcattgaag ggcataaagc attcctgttt gattcgcctt gggatgatac tcttactgag
    10201 atactggtta catacctgga agaggagatg ggcctgaaaa ttaagggctt cgtcccgaac
    10261 cactggcatg acgactgcat gggaggactt ggctacctga agagccggaa gataatttca
    10321 tacgctgaca gcctgacgct gctgttagcg agggaaaata agctgcatgt gccggatcac
    10381 ggattcaagg actcacttat cctcaggctg ggaaggaagg agatccgttg ttacttcccc
    10441 ggagccgcac actccacaga taatatagtg gtatggatac cctccgaaaa aatccttttc
    10501 ccgggttgca tctgcaagag cgccgattca cagaatctcg gaaacacaac cgagggagac
    10561 ctcgcagcgt accctgcaac agtggaatgg attatcagga agttcccgga tgtgaatact
    10621 gtcattccgg gacatggagc atttggcggc cctgagctgt tgacgcacac acttgcacta
    10681 acacgcaaca aataagttca gaagatgtca cagaataatg atgaagaacc gaaatcaggc
    10741 atcgggcagt tgggactgac gccggagatg aacagctata taattgataa cctgcctgaa
    10801 ggaacgcttg tcgccagggt aatccgcgag caccgtgaac ggtacgccgt ctctgacggc
    10861 gaaaacgaat atgatgcaga gatcaccgga aacatgagat actcggccgc ttcacggaag
    10921 gactttcccg ctgtgggcga ctgggttacc attacaccgt ttgacaacga cagggccatt
    10981 ataaaccaga ttctgcccag gagatcggtt cttgcccgcc aggcagtggg gaaacccgga
    11041 gagattcaga tcatctcagc aaatattgat tatgccttca tagttcaggc aatcagcaat
    11101 aacttcaata tcaacaggtt ggagcgatac ctgaccattt gcaattcagc cggcattgag
    11161 ccgttgctgg tgctgagcaa gatcgacctg gtaagccggg aggtgactga tgaagctgta
    11221 agggctctcg caaaacgtga caggcatatg aagctcgtcc tgttaagcaa tgtaaccagg
    11281 gagggcgttg acagtgtgat cagcctgatg gaaagaggaa ggacatactg cgtgatcggg
    11341 tcatctggag ttggcaagtc gactctcatc aacacccttc ttcaaagaga ggccctgagg
    11401 accggcgcga taagcagcag cacgaacaag gggaggcatg tgacggaaca ccgggaactg
    11461 tttgtacttg agaacggcag catcatcatt gacaccccgg gaatgaagga actgggcctg
    11521 acagatgatt ccaccgggct gagcgccaca ttccgcgaaa ttgccgatgt ggctgcggag
    11581 tgcaggtacc ccgactgccg gcatgtaagc gaggccggat gtgcagtgct ggaagctgtt
    11641 gaaaaaggtg acatcgacag ggactcatat gaaaattatc ttaagatgct taaggagcag
    11701 gagaggttta ccaccacggt cgcagagaag agaaggaagg aaaagcactt cgggaagatt
    11761 ctgaaggatt accacaggat tcaaaggaaa aaccaggaag agtgagagga attgagattt
    11821 gcgaattgcg aattgcgagt gtcgagtttt gaaaaaaaat ctgaatacag tcatgaacga
    11881 ggaatcgtat aaccggaggc atcacactga tgttgctttc cgggctgctc cgtgattatg
    11941 acggaagcag gcagattgaa ctggcagcct ttaacggtga ggattatttt gcggttcccg
    12001 gccagatgaa ttacatcgca gccaacgagg gaaagtttgg caatatgatc cttgacatca
    12061 acattgatgg tgccggctat caggagggac cctcagcatt ctcggagttc aacctgccgg
    12121 aggagctgtc tgtaaaactg gcgcgcataa ttgacagata tcccggaata acctccggac
    12181 cgcagttgcc tcagggcgat catagcatat tcgtgcagtt cggggtgcct gccgttgccg
    12241 tgacctcgaa gtggttcatt gacaacatgg acaaccagga tgtgacccat accccgaagg
    12301 acaggcctga gataaccgac tgccacagac tggttgagat agcaaatgca ctgaatgaat
    12361 ttatcaggat gatttagttt tgaatttcat ccatgtaaat cacaactaat tgataacttg
    12421 gatcagatat ttggtattta tactgacact aatccgttgt gcaaatgaaa acaagaattt
    12481 tgtctttcct ggtgatgatg ctgctgggaa gcctgatgca gacatctgag gctcaggctc
    12541 ctgctgcacc ggtcaaaccg gcgctgctgg taattgatat tcagaatgcc ttcctgtcta
    12601 tgatccctga ggctgataaa gcaaaggcga tggccaatat cagctattac atgtccgtgt
    12661 tcaggcagca cggttacccg gtgatccttg tctaccatca cggggaggaa tatggagtta
    12721 agcctggtac tgagctcttc gagtttcctg aatcggtgcc cataagacct gaagatccaa
    12781 aggtaatcaa gacctatcct gatgcattca acaagaccga ccttgacaaa gtgcttaagg
    12841 agcacggctg caatacgctg ttcctgacag ggctcagtgc tgtgggctgt gtgctggcca
    12901 cctatgtcgg gtcaatgaat aatgattaca gggcattcct ggtaaaggat gcaatcatga
    12961 gccacaattc agagtacacg cagaacatag agataatgtt tgaggcggtg ggctcggaag
    13021 tggtgaagat ggttcttgag acatcaggaa agtgactgac ctgcctgatg gtacgggctc
    13081 tgtcagatac gggggaataa aaggcccccg ggaagctctc acgggaaacc gctaagtaca
    13141 aagaggctgt ccggaatctc tcccggaaaa ccagtgagca caaaaaggcc gcccggaaca
    13201 tctttcatga taccggtgag caaaaaaaac cgcccggaag atccagggcg gttagtgtat
    13261 aaaaaaagaa cgtgccggtt attcctttat aaatctcttc attatggttg cccggggaga
    13321 gatgaaccgc atgaaataaa tccccctcct gagcagagaa acctcgatga tcatctggtt
    13381 gtgattgtcg catacctcgt tcaggcattt attgccggtt acatcaaata tctcaatcat
    13441 cgtgatgcct tcaatatggt cggcaatcag ttcactcttg accgggttgg gataaatctt
    13501 gattctttca gcgagattgt gtgccacgga atcggtaacg gctgacctgg cctggaccgg
    13561 ctgaccgtca cctgtttgtc cggcagctgt gctcagcgac attatgagca ggcagcaagt
    13621 gattgatgac aagataattc cgggtaaatg tcttttcata ggcgaattgt caatactttt
    13681 gcgaggttag tcatcatcgc atcaaattta actgatgcaa ctgcacttta tactccccat
    13741 ctggtataaa tgtttcaaac tttaacaagt tatcaacaaa atttttttac aggatctccg
    13801 ttaggtcccg gatcagaatt catgaaccgg gggaggcatg ataagtcata gaaggttttg
    13861 cataaaaaaa ttacctttgg gccctgaatt ataatcacaa aaaaacttaa cgtgaaaaga
    13921 gcatttatag taatgatgac agttgtcctt gcagctgttt catgcaacac acagaagaag
    13981 gagcagggca atcccttttt cgctgaatgg aagaccccgt tcagcgttcc gccctttgac
    14041 cagatcaaga acgagcacta tcttcctgcc atcgactcgg gcatcgtcct tgccaggctg
    14101 cagatagctg agatcaccgc aaatgcagag gcaccgacat ttgccaacac tgttgccaga
    14161 tatgacaggg caggtgaact gatgtcgaag gtggcctatg tattctactc gcagaccagc
    14221 gcaaatacca atgatactct tgaggcgctg cagatggaga tagctcccaa agtgtctgct
    14281 ttcagtgatg aggttttact gaacgcagaa cttttcaacc ggattaaggc ggtttgggag
    14341 aacaggtcag ctgaacagct gaatgacgag cagctcttca tgctcgaaaa catgtataag
    14401 agctttgtgc gcaacggagc cctcctcggt ccggaagacc aggagaaact taagaagtta
    14461 aaccaggaac tgtcggtcct gaccgtcaaa ttcagccaga acgtccttga ggagaccaac
    14521 agcttcaggc ttgtcattga taacgaggct gacctgaaag gtcttcccgg gagtgtcata
    14581 gccggtgctg cagacatggc gaagagcgat ggcatggatg gtaaatgggt tttcaccacc
    14641 cagaaaccga gcatgattcc gttcctgcag tatgctgaca accgtgatct gcgcaagaag
    14701 ctctatgatg catacctcaa ccgcggcaac aacggaaatg agaatgacaa taacaagata
    14761 atagccgaca ttgtaaggct cagggcagag agggctaaac tactgggata caggacgcac
    14821 gctgacattg tccttgagac ccgcatggcc aagactcccg ataacgttct taatcttctc
    14881 aataaccttc ttgagaggtc actccccgtc gcaaagcgtg agcgtgatga gatgcagaag
    14941 attgctgacg tcgggggcgg caattttaag attgagcccc atgactggtg gtactatgcg
    15001 gaaaaactgc gcaaagcgaa gtatgatctt gacgagaacg agctgaggcc ttacttcagt
    15061 cttgacaacg tacgcgacgg cgcctttgcc gttgccaaca aactctacgg catcacgttc
    15121 acccgtatag agaacattcc ccttcctcac cccgatgcac aggcatttga ggtgaaggaa
    15181 gccgacggca gccatgtagg ggtgctttac atggacttct atccgcgtga gagcaagcgc
    15241 cagggagcat ggtgcggagg ttaccgtgac cacaaggtag ttgacggcag ggaaatcacc
    15301 ccggttgtaa caatagtggg caatttcacc cctcccacag gaggcaggcc ggcccttctg
    15361 agcatggatg acgtcctgac cctcttccat gagttcggtc acggacttca ggggctcttc
    15421 tcggagaaca cctactcatc aaccaccgtg gcatgggaca tagttgaact gccatcgcag
    15481 ataatggagc actgggctac gaagcccgaa gtgcttgcca tgtacgcgaa gcattacgag
    15541 accggtgagg taatccctga agcccttgtt gagaagatca ggaacagcag tttctttaat
    15601 accggatttg acaatgttga gatcatggct gcttctcttc ttgatatggc gtattactca
    15661 cttgaagctc ctgtgcaggt tgatccgcaa cagttcgaga aggggactat gaagaagatt
    15721 ggtctgatac cggagatcga gccgagatac cggagcacct atttcctgca catgattgac
    15781 ggctacgatg ccggctacta tgtgtacacc tgggctgcag tgcttgacaa tgatgccttc
    15841 gaggcattca acgagaaggg cctgtttgat caggcaactg ccacttcgtt caggaagaat
    15901 gtgcttgaga agatgggaat catggatgcc gaaaagatgt accttaactt ccgcggccgt
    15961 gagccggaga tcgaaccttt gctcaggaac aggggactga actgaccaac agcgttcagt
    16021 atgatattga ggaggctgcc atcctgcggg aagcagcctc ttcaatttat acaatctctt
    16081 tctggtactg cgagttgacg aacaggacag tgccggctgc atcataacct cccaggatgg
    16141 tctccccgtt ctgtctgagc gcttccggcc ggaaaccggt cattaccatg gcagcggtgg
    16201 atgacttttc attgataagg cttttgacag agctgccatg agtgacctcg atgatctcca
    16261 cattcttttc ggttatcgga agccggcccg aggtaatcag cccctccagt tcccgcctgg
    16321 tctcctcata ctgacccggt ttgcatgtgc tgaatacctt gatgaatgat ttcttccagt
    16381 ctggatgacc tgagatgata aagctcaaca gtatcagcag gcttgcattt tcaggctcgt
    16441 tgaagtcgat ccatacgtga atgcccttgt tgcagatcat ctccctggaa gccgaggcaa
    16501 gcaggcagat gtcaaagtct cctgccttga tgagacggat attgtcaatg atctcaggca
    16561 gattctcggg attctccttg tcatattcaa agacaaccat attgttctcc atgccggaga
    16621 tgcccggcac ctgtatggcc tggacaattg ccgaggtata tgagggagat atgattgtgt
    16681 caacgtagac gtggttatgt gaggtctcat acaattctat cagttttgcc agctcctccg
    16741 tggcctgctg actcgtggac cgggaatagt atccgtcaat ccggtgaagg taggtgccga
    16801 aaccatagct gtacgagagc cagttcagaa gccggaaagc cctttcccgc ctgaaagagt
    16861 cctttgagat gcaaatcacc ccgggcctcc attccttctc ctttcgcctg ctcccgctct
    16921 tttgcaggta aacctgcagg ttacggttga tctggaagag ggtgttggcg aagagcgatt
    16981 cgaggcctgt tctgtttttg tgataatgat ttatgtatga atagattatt acaatgagca
    17041 cggaagcggt cagggcataa agggtattta tcctgaacat cacaatgact gcaaccacaa
    17101 agcctgtcag tgacaggtac catttcgacc tgaatgatgg tctgtatgaa ggcgacgagc
    17161 cgaagtggtt aaggaatgat atcaggcata gtgaaccgta tgttacaagg aagaacatgg
    17221 aaattatttc agccaccgcg ttgacatttc ccagtgcaac aaatgcagat gctatcagca
    17281 atgtcaccag tgtggcattc accggttccc cgtcattgtt tcttgctgcc gcgagccacc
    17341 tgttgagtct catcgaggaa aatgaggtgt cttttgccag cgcctgcagg gttctgggtg
    17401 ccaccagaac agagcccagt gcagaggaga gggttgaggc tgccaggccc aggggaataa
    17461 tgacagttcc accgatggct atctttccca tgatcagctg gttttcaagc atctcttcag
    17521 gggaggccga gatggccagt ttccagatca caagtacgta aaccactatg cctgtcgctg
    17581 ttgccaggat ggtgccaaga gggatggccc gggcgggatt cctcaggttg cctgacaacc
    17641 ccactccggc agtcatcccg gtgaaagcgg ggaagcagat ggcaaagaca atgaagaaat
    17701 cgctcatatt gcggatatcc ctcaccggca gctgccctgt agccaggctc ccctgggcag
    17761 gcttgccgag gaagaagagc aggaggctga caaagaggat ggcaatcaca gcatagagca
    17821 tcttcacacc ggtgttggat cccttcctga gaatcatcag tccgagaatc acagtcaccg
    17881 gcaggcttac cacctgcctg ggcaaaacaa aggagaaccg ctgtgcaacg aaattgaaca
    17941 ggaactcgaa cgactcggta aaggcaatga tgtaaaaggc cacgctgatt gcctgtgaaa
    18001 ggtaaagtgc aataccaatg gtggcaccga tgttcagtcc gaacgacctg gagattatga
    18061 agtattctcc tccgccttca accctcttgt tggtggcaag ctccgagata gccagggcgg
    18121 tggggatggt cacggcgtgt cccatgacaa tcagcaggag tgttccccag aatccgagcg
    18181 tccctacggc aaagccgaag cgcaggaaca gtatggctcc gagtatcgtt gatatggccg
    18241 tgaaatagac aggggccgtt ccgaatttct gcttggcaac tgttgcttcc atcagggcag
    18301 gtctgtaact ccggtatagt tacttttcta aaatataaag atgtaaaaaa atgtctgaaa
    18361 aaagttattt aatatcttta tatcggggta aaaacctata tgaaccagga aacggttgca
    18421 ggatcagatc tgaaggagat tgaggagttt ctaggcgggc tagggattga cgggcgtcat
    18481 tcgcgaaggc tgctttactg ggtctaccgg aggggcatca ggtcatttga agagatcaat
    18541 gacatcccgc tgaaatttct gcgcgtcttg tcggaaaatg cagtccccgg cctgagtttg
    18601 cccattgctt cagaggtttc ctctgacggt tccgtaaagt atcttttcac cacaaaaaac
    18661 ggcatggccc atgagacggt atttctgcct gacggcaaac gccgtacggt atgtgtttcg
    18721 gtgcaatcgg gctgccggat gggatgccgt ttctgtgcca ccggaaagaa cgggtggaat
    18781 ggaaacctga ctgccggtga gatagtaaac caggttatca gcatccccca tgagataac
//
LOCUS       DAOPXU010000053        18400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_3170_length_18400_cov_13.292886, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000053 DAOPXU010000000
VERSION     DAOPXU010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18400
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3170_length_18400_cov_13.292886"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PKK03_06930"
     CDS             <1..284
                     /locus_tag="PKK03_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNX84172.1"
                     /translation="SPEMEFLKAAMDKSQEVIDGVVHMILYKGNIIIEGRESPSSLYN
                     KELSSMDIEGGFNQADSLGFIRINAIRLMAHHAIMEANKKKVTEDALVG"
     gene            268..1722
                     /locus_tag="PKK03_06935"
     CDS             268..1722
                     /locus_tag="PKK03_06935"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNX84173.1"
                     /translation="MHLWDKGGTTRESIISFTCGSDRLYDARLAPWDIVGSMAHAVML
                     ERCGLITAGEKESLLAGLARLLDEVTQSDFSIGKEYEDIHSWVEIRLGEMEGEGGNPG
                     EGRPGTQIARSAAGKVHTGCPASQIARSASGKLHTGRSRNDQVLTDLHLYIRHQSFLL
                     AHELKDLAVDFLDLGEEHKHDLMPGFTHMQAAMVTSFGLWFASYAESLCDDLHVLAGA
                     AALADASPLGTAAGYGTSLPIDRELTASLLGFKRLIVASPYAQMSRGRTERQLTGAIA
                     SAANTIGRFASDVILFMSPGYRFVSLKDDLTTGSSIMPQKKNPDLFELIRARCNRLQA
                     VPGEVMLMTAGLPPGYNRDFQELKAVLFDAFDGITGLVQSVHGALAGLVIRKDILSDN
                     SYNDIFSAEEANRMVREGIPFRDAYREVAKKAGSGSFTVPSTSQYTHTGSIGNPGTEL
                     VRERLEEVTGRFVAGNSPQELADRIREEGDQVPG"
     assembly_gap    1779..1878
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1933..3030)
                     /gene="carA"
                     /locus_tag="PKK03_06940"
     CDS             complement(1933..3030)
                     /gene="carA"
                     /locus_tag="PKK03_06940"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNX84174.1"
                     /translation="MRNNDRLSVRLTLEDGTVYRGWSFGAPVAAAGEVVFNTAMTGYP
                     ESLTDPSYRGQILCLTYPLVGNYGAPGKSEQDDLYMFYESSGVHISALIVSDYSFEFS
                     HWNAIESLDEWLRRSNVPGIFGIDTRALTKRIREKGAMLGKVEPEGMTTDFYDPNKVN
                     LVAEVSTSARKVYGNGKYRILLVDCGVKFNIIRNLLKRDTTIIRVPWDYDYSVEEYDG
                     LFLTNGPGDPKMCRAAIDNIRKSLDDDRPIFGICLGNQLMALAAGADTYKLRYGHRSH
                     NQPVQMVGTDRAYITSQNHGFAVNNNTLPAGWVPLFVNVNDNTNEGMKHVSRPFFSTQ
                     FHPEASGGPTDTDFLFDLFIENIERAGGSRH"
     gene            complement(3027..4202)
                     /locus_tag="PKK03_06945"
     CDS             complement(3027..4202)
                     /locus_tag="PKK03_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HNX84175.1"
                     /translation="MKYNFDEAPCRENTNCLKWDKREEVFGRADVIPMWIADMDFSTP
                     PFIIEALRERLNHEILGYSYRPDSYFNAFINWVSGLHGWDVRQEWIEFSPGVVPALNI
                     CTLAYTSPGDEIIIQPPVYPPFYGAVNDHGRRLVFNPLLETEKGYLMDLEGLRKIITP
                     ATRMLILSNPHNPVGRVWTRDELNSLAEICHEKGIVILSDEIHSDLLLPGMKHIPLAS
                     VSEKAAAVTVTCMAPSKTFNLAGLSTSSMVITDPVLMDKYRKTLIGLHMHLGNIFGNV
                     ASEAAYTHGREWLGQLMRYVEDNVDLVMSFCHERLPVIRPVRPEATYMIWLDCRAMGM
                     NGQELNRFFVDSAGVGMNEGSRFGPGGEGFMRMNLACPRVTVLRALEQIETALKTAR"
     gene            4394..5215
                     /locus_tag="PKK03_06950"
     CDS             4394..5215
                     /locus_tag="PKK03_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84176.1"
                     /translation="MKKTFTKLLVAVLILTVAGASACKNRGESRKQKEINAVELEALE
                     QKIEENVYPLPTSAEVIKKLTDMDLGYILGATNPPANARNYVESYNRSVNLGVYGADL
                     SYATLYNMQQDVIDYLTAIRTLALEQNLSKIYDESLYDRIKANFDNRDTLVTILTDAF
                     DRTYSYMVEAGQANLALLMVGGAWVEGMYLTMSVSESGAHLSGFESALLDQKKSFEEF
                     EGLAAPHNSDPLVSRILTSMQPIRDVYTKLGTSLTMEDIENLKKAVNSVRSDLIK"
     gene            5271..8393
                     /locus_tag="PKK03_06955"
     CDS             5271..8393
                     /locus_tag="PKK03_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX84177.1"
                     /translation="MRESVLHALIHIFAIISTVNPAGVTTRGKKILRSYLRRYLNQEL
                     EEEYFKLYENNLHFYERELKAVEEGELSDEDSLITFQITNICRQIKKGLFIEERMIVF
                     LQLLEFVFEDGTVSEQEYSIIDIVARTFNISKEEKTNAHAFMLGMRFDKITPASFLAI
                     EGESNIGEPSSRYANYHEWHHMVSRGMRGTIYVLFIRSTGHLLFMYDGKMQLHFKGRN
                     VVPLRPFLLEPGVNIRGHGMGPLYYTAIFRKFIKANFVEDIIFEGQSIEYHFKGSDNG
                     IQPMSFSVKSGNLVGIMGGSGVGKSTLLNILNGKLKPENGAVYINGYDLHNEPDELNG
                     LIGYVPQDDLLIEELTVYQNLYYNARLTFGDYDEAKLNQVVVDVLKELDLYEFRDMLV
                     GDPLNKKISGGQRKRLNIGLELMREPAVLFADEPISGLASHDSGNVMELLKDQAINGK
                     LVFVIIHQPSSDILKMFDRLWVLDRGGYMIYDGDPVDAIVYFKTETSQANAAESECPT
                     CGNVETDEILQIVEAKVVDPDGRKGQERQVSPVEWYHRYRQKMEPTLSGRPEMKPLPA
                     SNFKLPKRKKQVATFVRRNLVRKYADKQYMVINLLEPPLLAFILAFVSKYLADSVYLF
                     SDNKSYPVFLFMSVVVALFLGLTVSAEEIFRDNKILEREKYLNISRSSYLFSKITFLF
                     GLSAVQTLMYVLISQRILQVEGMMWRQWLILFTTSCFGNMVGLNISAGMKSVISIYIL
                     IPLVLVPQLLLGGAMIRFDDLHHSLTRKVYVPVIGDVMTTRWAYEAMAVEQFRSNRYM
                     KPFFETDMMISRYDWQSGYLVNELKRKSNEAAYASGKPEYEDVYRNSLEKLKTHIGEL
                     STETGLDPAQAMRVINREPFTRDASYIVNDYLDSLQKRIRRKFLEQTAVKDSITNALV
                     TSMGKDELVRLKTATYNNDLADIVLNRTVQSKFYETEDRIIQKSDPVLMLPDSRIGRA
                     HFYAPYKIIGNIRIGTLWFNMLVIWLLNITLFVTLYFNLLKLLLNLMERIKIPGFGSD
                     RLVPPWELVK"
     gene            8402..9178
                     /gene="rsmA"
                     /locus_tag="PKK03_06960"
     CDS             8402..9178
                     /gene="rsmA"
                     /locus_tag="PKK03_06960"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNX84178.1"
                     /translation="MPDHQVRPKKYLGQHFLKDKQIAAAIVDALRADGCDSVVEVGPG
                     MGVLTHLLAERELPAFRVVEIDRESVDYLRHNVTGPVEIIEGDFLELDLHPMGNRIAV
                     IGNFPYNISSQIFFKILDYRDHVSETVGMVQKEVAERICSQPGSRVYGILSVLLRAWY
                     DIEYLFTVSEHVFSPPPKVKSAVIRLTRNSVERLGCDEKLFVSVVKRCFNQRRKMLRN
                     PVKAMVKPGAGDLPLLSRRAEQLSVSQFVELTNWVEENRV"
     gene            complement(9190..10437)
                     /locus_tag="PKK03_06965"
     CDS             complement(9190..10437)
                     /locus_tag="PKK03_06965"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX84179.1"
                     /translation="MWLKMFKESFLFAFGSVAANKLRTFLSLFGITIGILSIISVFTV
                     FDWMEKSIKDSIATLGDNTIYVEKWPWTSDANIKWWDMMKWQPPSVEDYEAVVKRSAS
                     AEAVCYTVGSSEAIKYRGNLASDTYIFAATHDFENVRSFEIEKGRYFTLSESASGKPV
                     AILGYEVADKLFGNVQPVGREITINKYKAQVIGVFKKEGKGGMSDNGMDQAVLVPLNF
                     SRQFINFRNPFLNSDIMIRGKEGVSVQQLSDEVIMILRAARRIGPGKENTFSINRASQ
                     ITSTLEPVFRGINIAGWIIGGFSILVGGFGIANIMFVSVRERTSIIGIQMALGAKRRW
                     ILQQFLTESVLLSITGGILGLILVFIGTLFVNYFFTLGMHLTAGNILLAVVISATIGI
                     VAGFAPASSASRMNPVEAIGYSF"
     gene            10597..12123
                     /gene="purH"
                     /locus_tag="PKK03_06970"
     CDS             10597..12123
                     /gene="purH"
                     /locus_tag="PKK03_06970"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNX84180.1"
                     /translation="MMSDIKIKRAFISVYYKDGLEAIVKKLSELGVAIISTGGSWDFI
                     TASGVPAGKVEEITTYPSILGGRVKTLHPGVFGGILARRENETDMKQVTEYNIPLFDL
                     VIVDLYPFGETVASGASDQEIIEKIDIGGISLIRAGAKNWADVLIISSRNQYGSLMEI
                     LDNGKGTVTSEERYRFALEAFAMTSHYDSAIFNWFNRNGDATVFSKSITESHTLRYGE
                     NPHQKGVFFGDPDRSFVRLHGKEISYNNLLDIESAISLIDEFDDVTCAIIKHNNACGV
                     ASRPALKEAWEDALACDPVSAYGGVVIVNREVDEIAAAEMDKVFLEVVMAPSFTPGAV
                     KILSSKKNRIILQRRSAGNGRESFRSMLGGVLWQERDSSTEQASGMRPVTARQPSEEE
                     YLDMVFANKIVKHSRSNAIVLAKNRQLCASGIGQTSRIDALRQAIQKAKAFGFSLEGA
                     VMASDAYFPFADSVTTAHEAGIAAVVQPGGSVRDQESIDYCNKTGVAMVFTGVRHFKH
                     "
     gene            12156..13178
                     /locus_tag="PKK03_06975"
     CDS             12156..13178
                     /locus_tag="PKK03_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNX84181.1"
                     /translation="MGLFSFLSQEVAIDLGTANTNIIANDKLVVDEPSIVAIDRNTGK
                     LIAIGDRARQMHGRTHENIRVIRPLRDGVIADFHAAELMIRGMIKMINTGPKFFSPSL
                     KMVVGIPCGSTEVEIRAVRDSSEHAGGRDVYLIYEPMAAAIGIGLDVEAPEGCMVVDI
                     GGGTTEIAVIALGGIVCNRSIRVAGDGFTADIQAYMRHQHNIKIGEKTAEDIKIAVGA
                     ALPDIENPPADFVVRGPNLMTSLPIEIPVSYQEIAYCLDKSLTKIEASVLQVLEQTPP
                     ELYADIVNKGVFLAGGGALLRGLDKRLTDKINIPFRVSEDPLHAVVRGTGVALKNVEK
                     YPFLMR"
     gene            13192..14016
                     /gene="mreC"
                     /locus_tag="PKK03_06980"
     CDS             13192..14016
                     /gene="mreC"
                     /locus_tag="PKK03_06980"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNX84182.1"
                     /translation="MRSLLNFLLRFKTLILFLVLEAVALVMISTSHNYHQTVFYGLSR
                     NISGFVARRLERGTYYFRLRSVNEELVKENTLLRRRLEGLTAMPAEGFSGVHDTISGV
                     NYTYLNARVISNSVNKQKNFITLDRGSKHGVHVGMGVASPSGVVGVVVGVSPNYSVVM
                     SLLNTDFRLSASIARNDYFGSLAWDGINYRYAKLSEIPHHVTVSEGDTIVTSGYSAIF
                     PAGLMAGTLTGDQNRGGDFISLKVMLSVDFKKLTDVYVIGNLTRDERETLEEEVVK"
     gene            14013..14516
                     /gene="mreD"
                     /locus_tag="PKK03_06985"
     CDS             14013..14516
                     /gene="mreD"
                     /locus_tag="PKK03_06985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNX84183.1"
                     /translation="MNSILRYTGAFVLLVVLQLLIFNNIEFSGYVNPYVYVMFILILP
                     VSIPSWILLLLGFLTGFVIDIFTGTMGVHTFATVLAGFVRPWVLSLNVTAEVAEGDMS
                     PSSSRSGLRWFFIYTVTVVLVHHLALFFVELFSLKHFFHTLLRVLLSTAVTTFFILLF
                     DFIRPHR"
     gene            14527..16362
                     /gene="mrdA"
                     /locus_tag="PKK03_06990"
     CDS             14527..16362
                     /gene="mrdA"
                     /locus_tag="PKK03_06990"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNX84184.1"
                     /translation="MKDRFANRKYFIMALVLLAAVVLIARLFYIQIVDKTYRVSAANN
                     ALRPVTQYPARGLIYDRNGKLIVYNQAAYDLLVIPVQTSAFDTTRFNEVLGITMDDFK
                     SRMKAAVSYSRRAPSVFMKMISSETYAVLQEELYRFPGFYVQARTLRKYTRPVAAHLM
                     GYVGEVDNKLIAKDRYYRSGDYIGVSGIEKSYEEHLRGRKGVNIFLVDVHNNIKGSYA
                     EGRYDTVAIPGTNLWSTIDLTLQEYGERLMQNKTGSIVAIEPSTGEILTLVSSPTYDP
                     SLLVGRVRSENFTALQADTVKPLFDRALMASYPPGSTFKIMNGLIGLQENVIKPTSLF
                     SCSNGYHAGSLTVGCHSHASPLNLPQAVAQSCNAWFCNAFRNILENPASGSIQDSFDK
                     WRAYLVAFGFGNKMGIDLPNELSGNIPTRSYYDRYYGKDRWKALTVISLAIGQGEIGA
                     TPLQMANMAAVIANRGSFYIPHVVKKIGDNGTPSPLYTQKYITGISPENFLPVIEGME
                     GAVNGGPGSTARGARLDSIIICGKTGTAQNPHGKDHSIFVAFAPKDDPKIAIAVYVEN
                     AGFGSTFAAPIASLMIEKYLTDDIKRKYLEDHVLNMVIKPSGKEQ"
     gene            16346..>17196
                     /gene="rodA"
                     /locus_tag="PKK03_06995"
     CDS             16346..>17196
                     /gene="rodA"
                     /locus_tag="PKK03_06995"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNX84185.1"
                     /translation="MEKSNNILARLDWVTVILWLAMMIMGWLNIYAAVYNEQHTSIFD
                     MSQRYGKQLIFIIAAIILAVMVMVTDNKLWFFFAWFIYGFFILLLILVLVIGKEINGA
                     RAWFGVGAFSIQPSEFAKFATGLALASYLNSKRQLLGIREMIAATAIIIAPVLLIAVQ
                     PDMGSVVTYFAFFVVLYREGMSIWVFITAILAVALFLLTLILGNLPVAIGLLVVAAIA
                     LLVTSGYLLTLKAAAVMAVTGGMAFLIGNYILDLDTVLIIVLAVFLAGVVYAWFIYRL
                     RMVNQAI"
     assembly_gap    17197..17296
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17297..17847)
                     /locus_tag="PKK03_07000"
     CDS             complement(<17297..17847)
                     /locus_tag="PKK03_07000"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HNX84186.1"
                     /translation="MNVLAHIYLSGDSDNIIIGNYIGDYVKGRDYLQYPEGIRKGIII
                     HRHIDAFTDRHPVVHRSKLHFVGRYRKYAGVIIDILYDHYLTREWDLFSRRPLESVTY
                     NFYRAMVNNFEILPESVREKMPFFIIDNWLETYQTRRGIRRVLKTLSGITSLPKETRW
                     AMRVWKKQYYSIEEDFMEFFPQL"
     gene            18096..>18400
                     /locus_tag="PKK03_07005"
     CDS             18096..>18400
                     /locus_tag="PKK03_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84187.1"
                     /translation="MLKRNVIIIGAAGRDFHNFNTYFRDNELYNVVAFTAAQIPDIDG
                     RKYPAGLAGKLYPDGIPIYSESDLPRLISELKVTDCVFAYSDVPYQRVMAVSAIVNAA
                     "
BASE COUNT         4525 a         4590 c         4723 g         4362 t
ORIGIN      
        1 tctcgcccga gatggagttc ctgaaggctg ccatggacaa gagccaggag gtgatagacg
       61 gcgtggtgca tatgattctt tacaagggga acatcatcat cgagggacgt gagtcgcctt
      121 cctcattata taataaggag ttgtcgagca tggatattga gggaggattc aaccaggccg
      181 acagcctcgg attcatacgt ataaatgcca tccgcctgat ggctcaccac gccatcatgg
      241 aggcaaacaa aaagaaagtg acggaagatg cacttgtggg ataagggggg caccacgcgg
      301 gaatcgatca tcagcttcac atgtggcagt gacaggctgt atgatgcgag gctggctccc
      361 tgggacattg tggggtcaat ggctcatgca gtcatgcttg agcggtgcgg gctgataacg
      421 gccggtgaga aggagagtct tcttgccggc cttgcccggc tccttgatga ggtgacgcag
      481 agtgatttct cgatcggaaa ggagtatgag gatattcact catgggtgga aatccgcctc
      541 ggcgaaatgg agggggaggg cgggaatccg ggtgaaggcc gcccgggcac gcagattgcc
      601 cgcagtgcgg caggaaaggt tcataccggc tgcccggctt cccagattgc ccgtagtgct
      661 tcaggaaagc ttcataccgg ccgctccagg aacgaccagg tactgactga tcttcacctt
      721 tacatcaggc atcagtcgtt tctgctggca catgagttga aggatctggc agtcgacttt
      781 ctggatctgg gtgaggaaca taaacacgac ctgatgcccg gattcaccca catgcaggca
      841 gccatggtca cctccttcgg gctatggttt gcctcatatg ccgagagtct ctgtgacgac
      901 ctccacgttc tggccggtgc ggcagccctg gctgatgctt caccgctggg gaccgcagcc
      961 gggtatggca cctcattgcc gatcgaccgc gagctgacag cctcacttct cggttttaaa
     1021 aggctgattg ttgcttcacc ctatgcccag atgagccgcg gccgcactga acggcagctg
     1081 acaggcgcta tcgcctctgc cgcaaacacc atcgggagat ttgcctccga cgtgatcctg
     1141 ttcatgtcac cgggttaccg gtttgtttca ctgaaagatg atcttaccac cggctcatca
     1201 attatgccgc agaagaagaa tcctgacctg tttgagctta tcagggcccg ttgcaacagg
     1261 ttgcaggctg tccccggcga ggtgatgctg atgaccgccg gcctgcctcc cggatataac
     1321 cgcgacttcc aggagctcaa ggctgttctg tttgatgcgt ttgacggtat aaccggactg
     1381 gtgcagtctg tgcacggagc gctggcaggg ctggtgatca ggaaggatat actgagtgac
     1441 aacagctata acgatatctt ctcagcagag gaggcaaacc gtatggtccg cgaaggtatt
     1501 cctttcaggg atgcgtatcg cgaggttgca aaaaaggcag ggtcagggtc gtttaccgtt
     1561 ccgtccacgt cacagtacac ccatacaggc agtatcggaa atccgggtac agagttggtc
     1621 agagagaggc tggaagaggt gacgggccgg tttgttgcag gcaattcacc ccaggagctg
     1681 gctgacagga tcagggagga gggggatcag gttcccgggt gatacaattc tgtatagcca
     1741 aagtcgcaca catcgcaaat cgcacatcgc acatcgcann nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnat cgcaaatcgc acatctgcaa tcctttatgg cctatgccca
     1921 atccactgct ccctaatgcc tgctgcctcc tgccctctca atattctcaa tgaagaggtc
     1981 aaacaggaag tcagtgtccg tcggtccgcc ggaggcttca gggtggaact gggtggagaa
     2041 gaaaggcctg gagacatgct tcatcccctc gttggtgttg tcgttcacgt tgacaaacaa
     2101 cggcacccag ccggcaggca gcgtgttgtt gttcacggca aagccatggt tctgtgaggt
     2161 gatgtacgcc ctgtcggtac caaccatctg aaccggctgg ttgtgactgc gatggccgta
     2221 acgtaattta taggtatcgg ccccggctgc aagcgccata agctgattgc ccaggcagat
     2281 gccaaagatc ggacggtcat cgtcaagcga cttccttata ttgtctattg cagcccggca
     2341 catcttcggg tcgccgggac cgttggtcag aaacagaccg tcatattcct caacggaata
     2401 atcatagtcc catggcaccc tgatgatcgt ggtgtcacgc ttcagcaggt tgcggatgat
     2461 gttgaacttc accccgcagt cgaccagcag gatacggtat ttcccattac catacacctt
     2521 gcgggctgag gtgctcacct cggccacaag gttaaccttg tttggatcat agaaatctgt
     2581 tgtcattccc tcaggttcta ctttgccaag catggctccc ttttcgcgga tcctctttgt
     2641 cagcgcgcgg gtgtcaatgc cgaagatgcc cggcacattg ctccttcgca gccactcgtc
     2701 gaggctctct atcgcattcc agtggctgaa ttcaaatgag tagtcggaga ctatcagggc
     2761 ggatatatgg acgcctgatg actcataaaa catgtacaga tcatcctgtt cgctttttcc
     2821 cggggcaccg tagttaccca ccagcgggta tgtgaggcac aggatctggc ccctgtatga
     2881 agggtcagtc aggctttccg ggtatccggt catggcggtg ttgaacacca cctcgccggc
     2941 cgcagccacc ggcgctccga acgaccaccc gcggtagacg gtaccgtcct cgagggtcag
     3001 ccgcacacta agtctgtcat tgtttctcac cttgcagtct ttaacgctgt ttctatctgt
     3061 tcaagggcgc gcagaaccgt cacccgcggg caggccaggt tcatgcgcat gaatccttcg
     3121 ccccccgggc cgaaacgtga accttcattc attcccacgc cggcgctgtc aacgaaaaat
     3181 cggttcagct cctgcccgtt catgcccatt gcacggcaat cgagccatat catataggtg
     3241 gcttccggcc gcaccggcct gataacaggc agacgttcat ggcagaatga catgaccagg
     3301 tcaacattat cctcaacata tcgcatcagt tgcccaagcc actcacggcc atgtgtataa
     3361 gccgcttcag acgcaacgtt gccgaatatg tttccaaggt gcatgtgaag cccgatcagc
     3421 gtcttccggt atttatccat cagtaccgga tcggtaatga ccatcgagga ggtcgacagt
     3481 cccgccagat tgaatgtctt gctcggcgcc atgcaggtca ctgtcactgc tgccgccttc
     3541 tctgaaaccg atgccagcgg gatatgcttc atccccggca acaacaggtc ggaatgaatc
     3601 tcgtctgaaa ggatgacaat tcccttttca tgacatatct ctgccagtga gttcagttca
     3661 tcccgggtcc acactcttcc gacagggttg tgcggatttg acaggataag catgcgggtt
     3721 gccggagtga ttatcttacg gaggccttct aagtccatca aatatccctt ctccgtttca
     3781 agaagcgggt tgaataccag ccgccgcccg tgatcattca ccgctccgta gaagggggga
     3841 tagacaggcg gctgaattat tatctcatcg ccgggggagg tgtatgcaag cgtgcagatg
     3901 ttaagagccg gcacaacacc cgggctgaac tcgatccact cctgcctgac atcccatccg
     3961 tgcagccctg aaacccagtt tatgaatgcg ttgaaataac tgtcaggcct gtaggagtat
     4021 cccagtattt cgtggttcag cctttcgcgg agcgcctcaa taataaaagg aggcgtactg
     4081 aagtccatat cggcaatcca catcgggatc acgtcggctc tgccgaagac ctcctcacgc
     4141 ttgtcccatt taaggcagtt ggtgttttcc cggcatggtg cctcgtcgaa gttgtatttc
     4201 atgagactgt aaatgctacg ggatgcaaag ttaacagaaa atgataatga acaaccggcc
     4261 ggatacctgt gtttaggatg catgaacgcc gggcctgcaa tggccttgaa taattctgaa
     4321 atattattcc ggccataaac ttaaaaagtc ttatttttac gtagccaaaa cacaagtacc
     4381 aaacatcagt catatgaaaa agactttcac gaaattactg gtcgctgtat tgattctgac
     4441 agttgccggt gcatccgcct gcaagaacag gggtgaatca aggaaacaga aggagatcaa
     4501 cgccgttgaa ctcgaagccc tggaacagaa gattgaagaa aatgtctatc cgcttcccac
     4561 atcggcagag gtgatcaaga agctcaccga catggacctc ggttacatcc tcggcgcaac
     4621 caatcctcct gcaaatgccc gcaactatgt agagagttac aatcgctcgg ttaacctcgg
     4681 tgtatacggc gctgacctca gctatgcaac gctttataac atgcagcagg acgtgattga
     4741 ttatctgacg gccatccgca cactggctct cgagcagaac ctgtccaaga tatatgacga
     4801 atctctttat gacaggatca aggccaattt tgacaaccgc gacacgcttg tgacaatcct
     4861 taccgatgcc tttgaccgta cctattcata tatggttgag gccggccagg ccaacctcgc
     4921 acttctcatg gtaggaggag catgggttga gggtatgtat ctcaccatgt cagtctccga
     4981 atcaggtgcc cacctgtcag gatttgaatc agcacttctc gaccagaaaa agtcgtttga
     5041 agagtttgag ggactggctg ctccgcataa cagcgacccg cttgtctcgc ggatactgac
     5101 ctccatgcag cccatacgtg acgtctacac caaactgggt accagcctta caatggaaga
     5161 catcgaaaac ctgaaaaagg ctgtcaactc ggtcagatcg gatttgatta agtaataata
     5221 aacagatacc tgaagaccgc aagtaaaaac tgcggtcttt ttttgaggtt atgagggaat
     5281 cagtattaca tgcgctgata catatttttg ccattatctc taccgtgaac cctgccgggg
     5341 ttacgacaag gggcaagaag atccttcgca gttacctcag gagatatctt aaccaggaac
     5401 ttgaggagga gtatttcaag ctctatgaaa acaacctcca tttctatgaa cgtgagctca
     5461 aggctgtgga agagggagaa ctgtctgacg aggactccct gatcactttc cagataacca
     5521 atatctgcag gcagataaag aagggactct tcattgagga gaggatgata gtcttccttc
     5581 agttgctgga gttcgtcttt gaagacggga ctgtctcaga gcaggaatat tcgattattg
     5641 acattgttgc gcgaaccttt aatatctcga aggaagagaa gaccaatgcc catgccttca
     5701 tgctcgggat gaggtttgac aaaataaccc ctgccagctt cctggcaatc gagggagaga
     5761 gcaacatcgg cgaaccctcg tcaaggtatg ccaactatca tgaatggcac catatggtct
     5821 cccgcggtat gcgcggcacc atttacgttc tcttcatccg cagcacaggc catctcctgt
     5881 tcatgtacga tggcaagatg cagctgcatt tcaagggacg taacgttgta ccgctgaggc
     5941 cattcctcct ggaacccggg gtcaacatcc gcggacacgg aatgggacca ctatattata
     6001 ccgccatttt caggaaattc atcaaggcca acttcgttga ggatatcatc tttgaaggac
     6061 aaagcatcga gtatcacttc aaaggctcgg ataacggcat tcagcccatg agcttcagtg
     6121 tgaagtcggg caacctggtg ggcataatgg gtggcagcgg cgtaggtaaa agcaccctgc
     6181 tgaacatcct caacggcaag ctgaaaccgg agaacggagc tgtctatatc aatggctatg
     6241 atctgcacaa tgagccggac gagctcaacg gcctcatagg ctatgtgccg caggatgacc
     6301 tcctgattga ggagctgaca gtctaccaga acctgtatta caatgcgcgt ctcaccttcg
     6361 gcgactatga cgaggcaaag ctcaaccagg ttgtcgttga cgtacttaag gaactggatc
     6421 tatacgaatt ccgggacatg ctggtgggtg atccgctgaa caagaagatc agcggagggc
     6481 agaggaagag gcttaacata ggactggaac tcatgcgtga gccggcagtt ctttttgccg
     6541 acgagcctat ctcaggcctg gcatcgcatg actcggggaa tgtaatggaa ctgctcaagg
     6601 accaggccat aaacggcaag ctggtcttcg tgatcattca tcagccgtcg tctgacattc
     6661 tcaagatgtt cgaccggctc tgggtgctcg acaggggagg gtatatgatc tatgacggcg
     6721 atccggtcga tgccattgtc tacttcaaaa cggagacttc ccaggccaac gctgcagaga
     6781 gcgaatgccc cacctgcggc aacgtggaga cagatgagat cctccagata gttgaagcca
     6841 aggttgttga tcctgacggg aggaaagggc aggagagaca ggtgtcgccg gtggagtggt
     6901 atcaccggta caggcagaag atggagccaa ccctctccgg caggccggag atgaagccac
     6961 tgcccgccag caatttcaag cttcctaaac ggaagaaaca ggttgccaca tttgtcagga
     7021 gaaaccttgt gagaaagtat gcagacaaac agtacatggt catcaacctt cttgagccac
     7081 ccctgcttgc ctttatcctt gccttcgtct caaagtatct cgctgacagt gtctacctct
     7141 tctcagacaa caagagttac ccggtgttcc tgttcatgag tgtggtggtc gccctgttcc
     7201 tcggcctcac ggtcagtgcg gaagaaatct tcagggacaa caagatactt gagcgcgaaa
     7261 aatacctcaa tataagccgc tcaagctacc tcttctctaa gatcaccttc ctgttcgggc
     7321 tgtcagcagt gcagacactg atgtatgtac taatatcaca gcgcatcctc caggttgaag
     7381 ggatgatgtg gcggcaatgg ctgatacttt ttaccacttc atgcttcggc aacatggtgg
     7441 gactgaatat atcagccggc atgaagtccg tgataagcat atacatactc atcccccttg
     7501 tgctggtgcc ccagcttctc ctcggcggtg ccatgatcag gtttgatgac ctgcaccatt
     7561 ccctgacaag aaaagtgtat gtcccggtga taggagatgt catgacaacg aggtgggctt
     7621 atgaagccat ggcagttgag cagttcaggt caaacaggta tatgaagcca ttctttgaga
     7681 cggatatgat gatcagccgg tacgactggc aatcgggtta cctggtcaac gaattgaagc
     7741 ggaagagcaa tgaggctgcc tatgcatcgg gcaagccgga gtatgaagat gtctacagga
     7801 acagccttga gaaactcaaa acccatatcg gtgagctcag taccgagacc ggccttgacc
     7861 ccgctcaggc catgagggtg ataaaccggg aaccgttcac ccgtgatgcc tcttatattg
     7921 taaatgacta cctcgattct cttcagaaga ggatacgcag gaaatttttg gagcagacag
     7981 ctgtaaagga cagtataaca aatgctctcg tgacatcgat ggggaaggat gagctggttc
     8041 gtctgaaaac ggctacctac aacaatgacc ttgctgatat cgtgctgaac cgaacagtac
     8101 agagcaagtt ctacgagact gaagaccgga ttatccagaa gtcggatccg gtgcttatgc
     8161 tgcccgattc acgcatcggc agggcccatt tctatgcacc atataaaata atcggtaata
     8221 tcaggatagg aaccctttgg ttcaatatgc ttgtaatatg gctgttgaac ataaccctct
     8281 ttgttaccct ttatttcaat ctcctgaagc tgctgctgaa cctgatggag agaattaaga
     8341 tcccgggctt cggatcagac cgcctggtgc cgccatggga actggtcaaa taggattcgt
     8401 catgccagac caccaggtaa gacccaaaaa gtatctcgga cagcatttcc ttaaggataa
     8461 gcagattgcg gctgccattg tagacgcctt gcgtgccgat ggctgtgact cagtggtgga
     8521 ggtcggaccc gggatgggag tgctgactca cctgctggca gagagagagc ttcccgcctt
     8581 cagggtggtt gagattgacc gggagtcggt tgattatctg aggcacaatg tgacaggtcc
     8641 ggtcgagatc attgagggtg atttcctgga acttgacctg cacccgatgg gaaaccggat
     8701 tgctgtcatc gggaattttc cgtacaacat atcaagccag atattcttta aaatccttga
     8761 ttaccgtgat catgtttccg agacggtggg catggttcag aaggaggttg cggagcgaat
     8821 ctgctcacag ccgggcagtc gcgtttacgg aatactcagt gtgcttctgc gtgcatggta
     8881 tgacattgaa taccttttca ctgtcagcga acatgttttt tccccgcctc caaaggtcaa
     8941 atcagccgtg atacgcctta cacgtaacag cgtagaacgt cttgggtgcg atgagaaact
     9001 gttcgtcagc gttgtaaagc gttgttttaa ccagaggcgc aagatgctgc gcaacccggt
     9061 gaaagcgatg gtgaagcccg gcgccggaga tttgccgctg ctctccagga gggcagagca
     9121 attatctgta agccagtttg tggagctgac caactgggtg gaagaaaaca gggtttgact
     9181 ggcccctgat cagaatgagt accctatggc ctcgacaggg ttcatcctcg aggcggatga
     9241 agcaggtgca aagcccgcaa caatccctat ggtagccgag ataaccactg caagcagtat
     9301 gttgcctgcg gtgagatgca tccccagggt aaagaaataa ttcacaaaaa gggtaccgat
     9361 aaacacgagt atcagcccca gtatcccgcc ggtgattgaa agcagaactg attcggtaag
     9421 gaattgctga agtatccatc tcctttttgc cccgagcgcc atctgaattc ctatgatgct
     9481 cgtacgctca cggaccgaaa caaacatgat gtttgcaatg ccgaatccgc ctacaagtat
     9541 cgagaatccc ccgatgatcc atcccgctat gttgatgccc ctgaataccg gctcaagggt
     9601 gcttgttatc tggcttgccc tgttgatgga gaaggtgttt tccttacctg gtcctatgcg
     9661 cctggcggcg cgaaggatca ttatcacctc atctgacagc tgctgaacgg ataccccttc
     9721 tttccccctt atcatgatat cagagttgag aaacggattg cggaagttga tgaactgacg
     9781 gctgaagtta agcggtacca gtaccgcctg atccatcccg ttgtcactca ttccgccctt
     9841 gccttccttc ttaaagactc cgatgacctg ggctttgtat ttgttaatgg ttatctccct
     9901 gcctacaggc tgaacatttc cgaaaagctt gtcagccacc tcataaccga gaattgcaac
     9961 cggcttgccc gatgctgact ctgacagggt gaaatagcgg cccttctcta tctcgaatga
    10021 ccttacattc tcaaagtcat gcgtggcagc aaaaatatat gtgtcactgg ccaggttacc
    10081 gcggtatttt attgcttctg atgacccgac agtatagcat accgcctcgg cagaggcaga
    10141 tcgcttcact acggcttcat aatcctccac cgaagggggc tgccatttca tcatatccca
    10201 ccacttgatg tttgcgtcag aggtccatgg ccatttctca acatagatgg tgttatcacc
    10261 aagagtggct atgctatcct ttatgctttt ctccatccag tcgaacaccg tgaatactga
    10321 gataatggat agaatcccga ttgtaatgcc gaaaagcgat agaaaggtgc gcagcttatt
    10381 ggccgccact gaaccgaagg cgaacaaaaa gctctccttg aacattttta accacatgtc
    10441 cccgtcttta attgcgatca aagataatta attatgaaac atcaatacat ccgcaataaa
    10501 ccatcaattc aattatttga taaacattgc caatcaaaaa aaatatctat ttttatccga
    10561 ttacttctgc cttggttcag ttaatctaag tcattaatga tgagcgacat aaaaataaag
    10621 agagccttta tttcggttta ttataaggat ggtctcgaag ccattgtgaa aaaactgagt
    10681 gaactgggtg ttgccatcat ttcaacggga ggatcatggg atttcatcac tgcttcgggt
    10741 gtgcccgcag ggaaggttga ggagataaca acctatcctt ccatcctcgg aggacgggtg
    10801 aaaaccctgc atcccggagt attcgggggt atcctggcaa ggagggagaa tgagactgac
    10861 atgaagcagg tgaccgaata caatattccg ctctttgacc tggtgattgt tgacctgtat
    10921 ccattcgggg agactgttgc ctcgggcgcc tcagaccagg agatcattga gaagatagac
    10981 atcggtggaa tttccctgat aagggccgga gccaaaaact gggctgacgt actgataatt
    11041 tcctccagaa accagtatgg cagcctgatg gagatacttg ataacggaaa gggcactgtc
    11101 acctctgagg agaggtaccg gtttgccctg gaggcgtttg caatgacttc acattatgac
    11161 tctgccatat tcaactggtt caacaggaac ggcgatgcca cggtgttcag caaaagcata
    11221 acagaatcac atactctgag atacggcgag aatccccacc agaagggagt tttcttcggt
    11281 gatcctgaca ggtcattcgt ccggcttcac ggcaaggaga tctcatataa caatctgctt
    11341 gacattgaat cagccatcag tctcattgat gagtttgacg atgtcacctg tgccatcatc
    11401 aagcataaca atgcatgcgg agtggcttcg cgcccggcgc tgaaggaggc atgggaggat
    11461 gcgctggcct gtgacccggt atcggcttac gggggcgtgg tcatagtgaa ccgggaagtt
    11521 gatgagattg ctgccgcgga gatggacaag gttttcctcg aggtggtgat ggccccctcc
    11581 ttcacgccgg gcgcggtgaa gatcctcagc tcaaaaaaga acaggatcat tttgcagcga
    11641 cgctccgccg ggaatggcag ggaatcattc aggtcaatgc tgggaggagt cctatggcag
    11701 gagcgtgaca gctccactga acaggcctca ggaatgagac ccgtcacagc aaggcagcct
    11761 tctgaagagg agtaccttga catggtattt gcaaacaaga tagtgaagca cagccggtca
    11821 aatgcaattg tgcttgcaaa aaaccggcag ctgtgtgcca gcggcatagg ccagacatcc
    11881 aggatcgatg cccttcgcca ggctatccag aaggcaaagg cattcggctt ctcactcgaa
    11941 ggagcagtga tggcttccga tgcatacttc ccctttgctg actcggttac caccgcacat
    12001 gaggcaggaa ttgcagctgt tgttcagccg ggaggttccg tacgtgatca ggaatcaatt
    12061 gattattgca ataaaacagg tgttgcaatg gtttttacag gtgtaagaca tttcaaacat
    12121 taaaatagac taacaagaaa cagtttcaag gacctatggg attattttct tttctttcac
    12181 aggaggttgc tatagacctg ggtacagcca atacaaatat aattgccaat gacaagcttg
    12241 tggtggatga gccatctata gtggccatcg acaggaatac gggcaagctt attgccatag
    12301 gcgacagggc caggcagatg catggcagga cccatgagaa catacgggtg atcaggccgc
    12361 tgcgcgatgg tgtcattgcc gacttccacg ctgccgagct gatgatcaga ggcatgatca
    12421 agatgatcaa cacggggccc aagttctttt caccgtcgct gaagatggtt gtcggtatcc
    12481 catgcggaag taccgaggtg gagataaggg ctgtccgtga ctcctcggag cacgccggag
    12541 gaagagatgt ctacctgatc tatgagccaa tggctgctgc aataggaatc ggacttgacg
    12601 tggaagcgcc cgagggatgc atggtggtgg atatcggagg cggaaccaca gagatagccg
    12661 tcatcgcact gggcggaata gtatgcaaca ggtcgataag ggtggccggc gacggcttca
    12721 ccgctgacat ccaggcttac atgcgtcacc agcataacat caagatcggt gagaagactg
    12781 ctgaagatat caagattgcc gtcggagcag cgcttcctga cattgaaaat ccgcccgccg
    12841 acttcgtggt acgaggcccc aacctcatga cctcgctccc aatagagatc cccgtatcat
    12901 accaggagat cgcctactgc cttgacaagt ctcttacaaa aatagaggca tcggtccttc
    12961 aggtactcga gcagactcct cccgaactgt acgctgacat cgtcaacaag ggcgtgttcc
    13021 ttgcaggcgg cggcgctctg ctgagaggcc ttgacaagcg tctcactgac aagatcaaca
    13081 tccccttcag ggtgtcggaa gacccgcttc acgctgtggt cagaggcacc ggggtggcgc
    13141 tgaagaatgt agagaagtac ccgttcctga tgagataaac cctgttcatg aatgagaagc
    13201 cttctgaact tcctgctcag gtttaaaacc ctgatccttt tccttgtgct ggaagcggtg
    13261 gctctcgtca tgatctcaac atcgcataat taccatcaga ccgtgttcta cggcctgtcc
    13321 cgcaatattt ccggatttgt tgccaggaga ctggaaaggg gcacttacta ttttcgcctg
    13381 cgaagcgtaa acgaagagct cgttaaggaa aacaccctgc tgcggagacg cctcgagggg
    13441 ctgaccgcca tgcctgcgga aggattttcc ggagtacatg ataccatcag tggtgtcaat
    13501 tacacatacc tgaatgccag ggtcataagt aattctgtca acaaacagaa gaacttcatt
    13561 accctagacc ggggatcaaa gcacggagtg cacgttggca tgggggtagc ttccccttca
    13621 ggagtggtcg gagtggtagt gggtgtctca cctaactact cggtggtgat gtcactgctg
    13681 aacaccgatt tccgcctcag tgccagtatt gcccgcaatg actatttcgg atcccttgca
    13741 tgggacggta taaattaccg gtatgcaaag ctctcggaga tacctcatca tgtgacggta
    13801 agtgagggtg atacaatcgt taccagcggc tattcagcaa tcttcccggc cgggttgatg
    13861 gccggaaccc taaccggcga tcagaaccgg ggaggcgact tcatctccct gaaggttatg
    13921 ctctcggtag atttcaaaaa gcttaccgat gtgtacgtaa taggcaacct gaccagggat
    13981 gagcgggaaa cccttgagga ggaggtggtg aaatgaacag tatcctcagg tatacgggcg
    14041 ccttcgtact gctggtagtg ctgcagctcc tcatattcaa taacattgag ttcagcgggt
    14101 atgtcaaccc ttatgtatat gtgatgttta tcctgatcct cccggtgtcg ataccctcat
    14161 ggatacttct cctgctgggc ttcctgaccg ggtttgtgat cgatatcttc accggaacca
    14221 tgggtgttca tacatttgca accgtactag ccggcttcgt ccgtccatgg gtgctgtctc
    14281 tcaacgtcac cgccgaggtt gctgaggggg acatgtcgcc ctcttcttcc cgcagcggac
    14341 tgagatggtt ctttatctat accgttacag tggtgctggt gcatcacctg gccctcttct
    14401 tcgtggagct gttcagcctg aagcatttct tccacactct gctgagagtg ttgctgagca
    14461 ctgcggttac cacattcttc atcctgcttt tcgacttcat caggccacac agatgaggat
    14521 gcggatatga aggaccggtt tgcaaacagg aaatacttta tcatggcgct ggtgcttctt
    14581 gcggccgtgg tactcattgc ccggcttttc tatatccaga tagttgacaa gacctatcgg
    14641 gtatctgccg ccaacaacgc cctccggccg gtaacgcaat atccggcacg cggactaatc
    14701 tatgaccgta acggcaaact gatcgtttac aaccaggcag cctatgacct gctggtgata
    14761 ccagtccaga caagcgcctt tgataccacg aggttcaacg aggtccttgg catcaccatg
    14821 gatgatttca agtccaggat gaaggctgcc gtatcatatt cgcgccgggc gccgtcagtt
    14881 ttcatgaaga tgatttcttc agagacatac gcagtattgc aggaggagct ttaccgcttc
    14941 cccggcttct acgtgcaggc cagaaccctc agaaagtata cccggccggt tgctgctcac
    15001 ctgatgggct acgtgggcga ggttgacaac aagcttattg caaaggacag gtattacagg
    15061 tcgggcgact atataggcgt cagcggcatt gagaaatctt acgaggagca tctcagggga
    15121 aggaaggggg tcaacatctt ccttgttgat gtgcacaaca atatcaaagg atcatatgcc
    15181 gagggcaggt atgatacggt ggccatcccg ggaaccaacc tgtggtctac cattgacctt
    15241 accctccagg agtacggtga acggctgatg cagaacaaga caggctcaat cgttgcaatc
    15301 gaaccctcga cgggtgaaat tctcaccctc gtttcatctc caacctatga tccttcactg
    15361 ctggttggaa gagtcaggtc tgagaatttt actgccttgc aggctgatac ggtcaagccg
    15421 ctctttgaca gggccctgat ggcctcatat ccgcccggtt ccacattcaa gataatgaat
    15481 ggcctcatag gcttacagga gaatgtcatt aaacccactt cccttttcag ttgcagcaac
    15541 ggttaccatg ccggttcgct gacggtagga tgtcactcgc atgcctcgcc cctcaacctc
    15601 ccccaggctg tggcacagtc gtgcaacgcc tggttctgca acgccttccg caatatactg
    15661 gagaaccctg cctccggatc aatacaggat tcatttgaca agtggcgggc gtacctggtg
    15721 gctttcggct tcggaaacaa gatggggata gacctgccaa acgagttgtc tggaaacatt
    15781 ccaacaaggt catactatga ccgttattac ggaaaggacc gctggaaggc cctcacggtg
    15841 atctcactgg ctatcggcca gggggagatt ggcgccaccc cgctgcagat ggccaatatg
    15901 gcagctgtaa ttgccaacag gggttcgttt tatattcctc acgtggtcaa gaagataggt
    15961 gataatggaa caccgtctcc cctctatacc cagaaataca ttaccggtat ctcgccggag
    16021 aattttctgc ctgttattga aggaatggaa ggagccgtca acggaggccc cggctcaacg
    16081 gcacgcggtg cacgccttga cagcatcata atatgcggaa agaccggaac ggcacagaac
    16141 ccgcacggga aagaccactc catcttcgtg gcctttgccc ccaaggacga tccgaagatc
    16201 gcgattgcag tgtacgtgga gaacgcaggt ttcggctcca cattcgctgc ccccattgcc
    16261 agcctgatga tagaaaaata ccttactgac gacataaaga gaaaatacct ggaggatcac
    16321 gtcctgaata tggtaataaa accaagtgga aaagagcaat aacatactgg caaggcttga
    16381 ctgggttacg gtgatactgt ggctggcaat gatgatcatg ggatggctga acatctatgc
    16441 ggctgtctac aatgagcaac ataccagcat ctttgacatg tcgcagcgtt acgggaaaca
    16501 gctgatcttc attatcgcgg caattatcct ggccgttatg gtgatggtta ctgacaacaa
    16561 gctatggttc ttttttgcat ggttcatata cggcttcttc atcctgctgc ttattcttgt
    16621 gctggttatc gggaaggaaa tcaacggcgc gagggcctgg ttcggagttg gtgcattcag
    16681 cattcagcct tctgagtttg ccaagtttgc caccggcctg gcgcttgcat catatctcaa
    16741 ctccaaacgg cagttacttg gcatcaggga gatgatagcg gctacagcta tcatcatagc
    16801 ccctgtactg ctgattgcag tccagcctga catgggatcg gtggtgactt attttgcatt
    16861 tttcgttgtc ctttaccgcg aagggatgag catatgggtg tttatcacgg caatccttgc
    16921 cgtggcactg ttcctcctga ccctgatact cggaaacctg ccggtggcca tcgggctgct
    16981 ggtggtagca gccatagctc tcctggttac ttccggatac ctgctgaccc ttaaagcagc
    17041 cgcagttatg gctgtcaccg gaggtatggc tttcctcatc gggaattaca ttcttgacct
    17101 tgacacagta ctgatcattg ttcttgcagt gttccttgca ggtgtggtgt acgcctggtt
    17161 catttaccgg ctgagaatgg taaaccaggc aatcatnnnn nnnnnnnnnn nnnnnnnnnn
    17221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17281 nnnnnnnnnn nnnnnnatca gttgcgggaa aaactccatg aagtcttcct cgatggagta
    17341 atactgtttc ttccagaccc tcatcgccca ccgtgtctcc tttggaaggg aagtaatgcc
    17401 gctgagcgtt ttcagcaccc tgcgtattcc cctgcgggtc tgatatgtct caagccagtt
    17461 gtcaatgatg aaaaatggca tcttctcacg tactgactcg ggcaggatct caaagttgtt
    17521 caccatggcc ctgtaaaagt tataggttac gctctcaaga ggcctcctgg aaaagagatc
    17581 ccattccctt gtcagataat gatcataaag aatgtcaatg atcacacctg cgtacttgcg
    17641 gtagcgtcct acgaagtgca gcttgctgcg gtgaaccaca ggatgcctgt cagtaaaggc
    17701 atcaatgtgg cggtgaatga tgattccctt gcgtattccc tcagggtact gcaggtaatc
    17761 acggccctta acatagtcgc ctatgtaatt gccgatgatt atgttatcag agtcgccaga
    17821 gaggtatatg tgcgccagaa cattcatccg ccgaaagtta gtgcaaagat tctgccaata
    17881 tgctgtggtt taaaatatta agaagagatt taatcaacaa acccgagtga tttattaact
    17941 tccataatat caaaatatta taagtctcag aaagattgaa tgaaactgac tattatcact
    18001 tccgatttca gttacaaatt ataaatttgc cggatacgtc aactgactta tgctgatatt
    18061 attttaaaaa aatcaataac taacgaggag gttatatgtt aaaaaggaat gttattatca
    18121 ttggtgccgc gggcagggat tttcacaatt tcaataccta tttccgcgac aatgagcttt
    18181 acaatgtcgt tgccttcaca gctgcccaga tacccgatat tgacggcaga aaatatccgg
    18241 ccgggctggc aggaaaactc tatcctgacg gcattccaat ttattccgaa agtgaccttc
    18301 ctcgcctgat cagtgaactt aaggttaccg actgtgtctt tgcatacagt gacgttccct
    18361 accagagggt aatggcagtg agcgccatag tgaacgcggc
//
LOCUS       DAOPXU010000054        18236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_3209_length_18236_cov_11.672295, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000054 DAOPXU010000000
VERSION     DAOPXU010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18236
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3209_length_18236_cov_11.672295"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..853)
                     /locus_tag="PKK03_07010"
     CDS             complement(47..853)
                     /locus_tag="PKK03_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84188.1"
                     /translation="MKNFLFWTIAIIITIAAAMYQRMTGPTYPYRTSASVDGTEYSFA
                     FKRSQTNTHPCLIKLEVPQEVSGTLYFRRFRTNDEWTPVVMKHDNGKLAGELPSQPAA
                     GKLEYYVVLRGASGSETALLREKPVVIRFKGNVPAVIMIPHILIMFFAMLLSNLAGIT
                     AVAGRDSQRKHGRLALWLLLAGGLVLGPLVQKYAFGELWTGVPFGWDLTDNKTLIAVI
                     AWVVAVIMNRKKARPAYTIAAALILLLVYSIPHSLFGSELDYVTGTVTQG"
     gene            complement(873..2639)
                     /locus_tag="PKK03_07015"
     CDS             complement(873..2639)
                     /locus_tag="PKK03_07015"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNX84189.1"
                     /translation="MRTGIIIFTALIMTACAGTGRKAAITTDELGWLAGELASDRFAG
                     RGTGTGGDSLAAMFIKRELGKAGTGPFTESGLQPFRVSVSVEAGTGNRLSVNGTDYLA
                     GTDFIPLALTSDSELNAPVIFCGYGLTPSGDTLSRNDFAGTDVSGKWALILRGYPESN
                     PSDAGYASLSSDRIKVLNAREKGAKGVLLVSGEKWDVADNLDKPSRNESTSGIPVFQV
                     KRNVADAILATTSVTVNGLEERAAGPGSSASMVTASIVDARAQIIKKEAVTSNVVMML
                     RGASAPEEYVIIGAHYDHLGMGGPGSSSRTPDTVAVHYGADDNASGVALMIELAERLA
                     AMKQRPARSILFVAFSGEEMGLLGSKHFVENSGISPAAVNLMINLDMVGRMKEGNGLQ
                     VAGIGTAAGLRDTVLAYADTTALSLAFTSEGYGPSDHSAFYGRNIPVLSITTGAHLDY
                     HTPMDTPDKLNYEGMIKTGDLLFGIVNAVAGRSARLAFTEAGPKEPTSPMGRRRGVTL
                     GIMPDFAGNVKNGLRADFVTPGKPAALGGMEKGDIIVSIDDKPVSNIEDYMFRLNQLK
                     PGQRVTVEVLRSGNRELLLIQL"
     gene            complement(2806..4017)
                     /gene="rocD"
                     /locus_tag="PKK03_07020"
     CDS             complement(2806..4017)
                     /gene="rocD"
                     /locus_tag="PKK03_07020"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HNX84190.1"
                     /translation="MKPHDYIEREEKYGAHNYHPLPVVLDRGEGPFVWDVEGKRYYDF
                     LSAYSAVNQGHCHPKIIGALTEQAAKLTLTSRAFYNSVLGEYEEYVTKYFGYDKVLPM
                     NTGAEGDETALKLCRKWAYLVKGIKENQAKIIVCEGNFHGRTITIVSMSTDPDARKDY
                     GPFTPGFITISYNDIPALEKALADPDVAGFLVEPIQGEAGVFVPDEGYIKKSYDLCKK
                     HNVLFIADEVQTGIARTGKLLACDHEGVRPDILILGKALSGGVLPVSAVLADDEIMMT
                     IKPGEHGSTFGGNPLACKVGIAALEVIKEEKLAEKAERLGKIFRDEMRAVKSDMIELV
                     RGKGLLNAIVIRPKNGKEAWDVCLAMKELGVLAKPTHGHIIRFAPPLVITEEQLRDAI
                     SLIKKAILLFA"
     gene            complement(4156..4842)
                     /gene="trmD"
                     /locus_tag="PKK03_07025"
     CDS             complement(4156..4842)
                     /gene="trmD"
                     /locus_tag="PKK03_07025"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNX84191.1"
                     /translation="MRIDIITVLPGLLRSPFDESIIRRARQKGIAEINIIDLRDYSTD
                     KHRSVDDYPFGGGAGMVMMIEPVYNCITRLKSERHYDEVIYTSPDGAGFTQAMANELS
                     MKENIIILAGHYKGIDQRIRDHLITREISIGDYVLTGGELPAAVITDAVVRLLPGAIS
                     DAESALSDSFQDGLLAPPQYTRPAEFMGWKVPDILTGGNTPEIERWRFEQSLRITREK
                     RPDLLGEEEG"
     gene            4929..5264
                     /locus_tag="PKK03_07030"
     CDS             4929..5264
                     /locus_tag="PKK03_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HNX84192.1"
                     /translation="MGKFVISKRKNGEFQFNLLAGNGQVILTSEGYASKPSCMNGVKS
                     VMKNAGDVKRFESKVAKNGKFHFNLKAGNGQVIGKSEMYESETSRENGIASVKKNAPG
                     ATIDDQTKA"
     gene            complement(5265..6953)
                     /locus_tag="PKK03_07035"
     CDS             complement(5265..6953)
                     /locus_tag="PKK03_07035"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNX84193.1"
                     /translation="MRQGILMIIALVLTAASGCTKAEKADLVITGGIVFTADSLNPRA
                     EAVAVKGNRILAVGSDREIGRYVDPQATKVIDARSRAVLPGFNDAHAHFGPVDPDYIE
                     LRYVTDPSVITNRVKEQVARSQKGQLIYGGHWEHEMFTTREWPSKELIDAVSPDNPVV
                     LRRTDGHSALVNSYVLRQSGITRSTPDPFGGEIMHDPVTGEPTGILKESAMSLIRTGA
                     KDIERTPEEEREREWSGYLLALKMATEYGITSIQIPGSADFDMYDSIMAAGKLSCRID
                     IGGELTADQARLDRYEALRQKYPAQGDWIRFGYLKDFMDGTLGSATAMMFEPFADAPD
                     RSGLPQMSYEELEARVLAADSRGFQIGIHAIGPKANNWILNAYEKAREVNGIRDSRHR
                     SEHAQILIEEDIPRFAGLGVIASMQPTHCITDKRFAEKRIGTERSRWAYAWKSLADSG
                     ARLAFGTDYSVEPLNPMEGLYASVTRKDRKGEEGDGWFPMEKITMEKAIEYYTLGAAY
                     AQFMEDRKGMIRKGYLADITILSENIFNTPEEMIMSVKSDYTIVDGKIAYERQK"
     gene            7049..8332
                     /locus_tag="PKK03_07040"
     CDS             7049..8332
                     /locus_tag="PKK03_07040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX84194.1"
                     /translation="MKSKVVPPGYLFTRRYSNYVFILLFLLYLFDYADRMVVSSMFSY
                     IQADLGINDFQSGWLVSIIYLTIGLLTFPVSLVIDRWSRTKTIGIMAIVWSLATLLCA
                     FTGNYVQLFLARVLIGFGEAGYAPGGSALISGMYPMEKRSKMMGLWNASIPIGSAIGV
                     TAGGIIASSWGWKHAFGLVAIPGLLIAILFLFVKDYKTVALSKVDETNNKIRMEKKDI
                     AKEFLKKPSVIFTYFAMTAIVFVTTALLVWLPKFFQVTSGMDPKKAGSLAGAVMMLAL
                     VGAPLGGYIIDRWRKTMPTARMIYPAISTLLSALLLFLALYVFRGTLQLVTLFIFGIF
                     IMQFISGAAAVTQDVIHPGLRATSYAIAVLVQNLLGSFTAPIALGRISDLTNIQTAVS
                     ILPFSLLLGGLLFLIGARYYIKDLKKVAPVTLEAE"
     gene            8592..9992
                     /locus_tag="PKK03_07045"
     CDS             8592..9992
                     /locus_tag="PKK03_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HNX84195.1"
                     /translation="MKELLRNKFFKFGIAGVLYLLWVIWVGNFWLLLGLPIVFDIYVS
                     KKVNWAFWKKRHGKNHVVIEWLDALIFAVIAVTLINIFLFQNYRIPTPSMEKTLLVGD
                     HLAVSKVAYGPRMPNTPLAFPFTQHTMPFTGGKGKSWSDLIHWKYKRLKGFGHVKNND
                     IVVFNFPAGDTVCLEQQAISYYEIVRERIETLTAEDIRAGRPLKSKDEYYSVARQMIW
                     NEYTVIYRPVDRRDNYVKRCVGIPGDTVLIKGGKLFINGHENAEFETQQTSYYVRTNG
                     TRINPKAFERMGVSKSDQSLMLSGTVYRLPLTKENAKTISGFANVTGIEADYHRPGEY
                     LASVFPHDPAYRWNEDYFGPLWIPKKGTTVKLDMTNINFYTSIIDIYEENDLEIRDTV
                     IYINGKPADSYTFKMDYYWMMGDNRHDSADSRFWGFVPEDHIVGRPVRVWLSIDKEAQ
                     GFKKIRFNRFFKAAKR"
     gene            complement(10083..10430)
                     /gene="rplT"
                     /locus_tag="PKK03_07050"
     CDS             complement(10083..10430)
                     /gene="rplT"
                     /locus_tag="PKK03_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNX84196.1"
                     /translation="MPRATNAPASKARRKKIFKQTKGYFGNNANVYSVAKNVLEKGLA
                     YSYRDRKDKKGQFRALWIQRINAGVRLHGMSYSQFIGGLEKKGISLNRKTLADLAMNH
                     PEAFKAIVDKVKE"
     gene            complement(10532..10729)
                     /gene="rpmI"
                     /locus_tag="PKK03_07055"
     CDS             complement(10532..10729)
                     /gene="rpmI"
                     /locus_tag="PKK03_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNX84197.1"
                     /translation="MPKVKTNPGAKKRFSLTGSGKIKRKHAFKSHILTKKSTKRKRNL
                     TYDGLVDKADQKNVKLLLGLK"
     gene            complement(10756..11298)
                     /gene="infC"
                     /locus_tag="PKK03_07060"
     CDS             complement(10756..11298)
                     /gene="infC"
                     /locus_tag="PKK03_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNX84198.1"
                     /translation="MFSRPRPGRKPEPEHRINNRITARTVRVVGENIEAKVMNLSDAL
                     RLADEMELDLVEISPNADPPVCKIVDYQKFLYQQKKKQKEIKANAAKIVVKEIRFGPN
                     TDDHDYNFKLKHAINFLEEGAKVRAYVFFKGRSILFKDQGEKLLARFVSDLDEYGKVD
                     QTPRLEGKRMIIILSPKKKK"
     gene            complement(11357..13297)
                     /gene="thrS"
                     /locus_tag="PKK03_07065"
     CDS             complement(11357..13297)
                     /gene="thrS"
                     /locus_tag="PKK03_07065"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNX84199.1"
                     /translation="MISITFPDGSVREYSQGITSHEIAMSISPGLAADVYAATVNGEV
                     RDLSRPVEGNATVRFHKWDDAEAKHAFWHSSAHLMAEALEALYPGIKFGIGPAIENGF
                     YYDVDPGDGKVITDSDLTLIEEKMKELAARKSPIVRTEVSKKEAMELFTAKGDEYKTE
                     LISELEDGTITLYRQGNFTDLCRGPHLPDTSSIRAIKVLSLAGAYWRGDEKRKMMTRI
                     YGITFPKQKMLDEYMTMIEEARKRDHRRIGKELELFAFSPKVGMGLALWLPRGADLKQ
                     RLIDFMTKVLKVRGYTMVSTPHIGNVDLYKTSGHFEKYGADSFRPITTPQEGEVFMLK
                     PMNCPHHCEIFNATPHSYRELPVRMAEFGTVYRYEQSGELHGLTRVRSFTQDDAHHYC
                     APEQLKEEFKSIIDLVQYIYRIVGFNEFTAQISLRDPGNHDKYIGTDENWEKAEKAII
                     EAAGEKNLVTTVAIGEAAFYGPKLDFMVRDAIGRKWQLGTIQVDYNLPERFGLEYTGS
                     DNQKHQPVMIHRAIFGSLERFVAVLIENTAGRFPVWLTPDQAVILPISEKFNDYAKTV
                     SNLLNNYDIRTLIDERNEKIGKKIRDNELKRIPYLLVIGEKEELAGTVSVRKQGEGDR
                     GVMKPGEFINLVKEEIKRETGI"
     gene            13447..14136
                     /locus_tag="PKK03_07070"
     CDS             13447..14136
                     /locus_tag="PKK03_07070"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX84200.1"
                     /translation="MKRILILTAAAALIMLNSCDTQPEAYFFADKIKAEIGEEVFFFN
                     GSYNATDYEWDFGDGTFSNAYEPAHVYNASGVFTVVLTAYTRSGNVDKSYLDIEINSP
                     TMLEVEVLEYYDQYPVSGASVILYPTETDWDNETNAIVEGFTNASGKVIFTNLQPRVY
                     FVDVWHSTHNNYTLRNEDTGFIRTDQLEKNQLNQFTAWVDYTGTKGAAIRDRKQLVPL
                     KSRTVSATVKK"
     gene            14228..15958
                     /locus_tag="PKK03_07075"
     CDS             14228..15958
                     /locus_tag="PKK03_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNX84201.1"
                     /translation="MDTKFALHGIDYLIMAVYFVFVLGIGWALKKYMKNATAFLEAGR
                     SIPAWVAGLAFISTNLGALEVMGMTGSGLKYGMLTTHFYWIGAIPAMVFLALFMMRFY
                     YGSKARSVPEYLKLRFDEKTRGLNAILFAVMTVLASGISMYALALLLENVLGWNFNVS
                     LFASAAIVLGYTYLGGLSSAIYNEVLQFFIIVIGLLPMAFLVMKDVGGWGGMQASLAP
                     IVESKGFAADAWTTTWKYTGTGSGNPLGVAWYGIFAGLGFVLSFGYWCTNFLIVQRAM
                     AAESMTAARKVPLIGAIPKMFIPFLIIVPGIAALVLLNDPLKGFALPLNDAGQPIYDY
                     TIIMLLKQYLPAGVLGLGVTALLASFMSGMAGNVSAFNTVWTYDIYQSYIRPATGNKE
                     ADDKHYLKVGKMATVVGVLVSIAAAYIASKFGNIMDFLQTIFSMINAPLFAVIFLGMF
                     WKRSTGNAAFTGLLAGFLMALVHHGLTQPEGATTLVKGGWLGVVHTYPVEMAQNFWTA
                     IIAFTVATLLTVFISLLSKRTKTDDELDGLVYSMTKKLDDSGVVWYNRPWFLALIVGV
                     IMIVLSILFW"
     gene            15976..16188
                     /locus_tag="PKK03_07080"
     CDS             15976..16188
                     /locus_tag="PKK03_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84202.1"
                     /translation="MLDIRFPIGLMFSIFGLIITIYGIATLGNDAMYARSLNINVNLV
                     SGIVTLLFGLIMLFFSGPVKKLLKRK"
     gene            complement(16291..17442)
                     /gene="lysA"
                     /locus_tag="PKK03_07085"
     CDS             complement(16291..17442)
                     /gene="lysA"
                     /locus_tag="PKK03_07085"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNX84203.1"
                     /translation="MITREITEKLGKHETPFYYYDLEMLRETLSVASGEAVNRGFHVH
                     YAVKANFNPVIMKIIAGYGFGADCVSGNEVEHAIACGFPAGETVFAGVGKSDAEIEKA
                     LRLDIKCFNAESAAELDVIDAIAGRLGKKAPVALRINPNVEAHTIKHITTGTDENKFG
                     IRIPELETVLDRLEGLQNLRYRGIHFHIGSQITDLHVYRNLCTRINELWQWLTDRGFG
                     PEDINVGGGLGISYDDPDRFPQFAEYFDVFRKHLDSRIQSTISFELGRSLTANCGSLV
                     SRVLFVKPGAAETFVILDAGMTELIRPALYHASHRIENLSSSGPVKKYTVVGPICETT
                     DTFGRSISLPETRRGDLVAIRSAGAYGEVMASRYNMRSLPMALFSEELQ"
     gene            complement(17439..>18236)
                     /locus_tag="PKK03_07090"
     CDS             complement(17439..>18236)
                     /locus_tag="PKK03_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNX84204.1"
                     /translation="IITQGFICRNVFGETDNLRRGGSDYSATIIGAAIAAEEVQIWTD
                     ISGLHNNDPRYVENTRVIRNLSFSEAAELAFFGAKILHPSSINPARMKNIPVRLKNTM
                     EPADDGTLITSETTLEDYTAVAAKDGIASIRIHSDRMLMAYGFMRRIFEVFEAFRTPI
                     DMITTSEVSLSLTIEDPAHLEDIVKSLSSIGQVETDRNQTIICVVGDFRPDRVGSAPE
                     IFEALNEIPVKMISYGGSDHSVSILINTADKITALRALNERLFTQKA"
BASE COUNT         4263 a         4748 c         4449 g         4776 t
ORIGIN      
        1 tgctgcatcc aaggtagaaa aaagctgtaa gaggatgaaa atttttttat ccctgtgtta
       61 cggtccctgt aacatagtca agctcagacc cgaacaggct gtggggaatg gagtaaacca
      121 gcaacaggat cagtgctgct gcaatggtat aagcaggcct tgccttcttg cggttcatga
      181 tgaccgcaac cacccatgct ataacggcga taagggtttt attgtcggtc aggtcccacc
      241 cgaacggaac tccggtccac agctctccga aggcatactt ctggacaagc ggcccaagta
      301 ccagtcctcc tgcaaggagc agccacagtg caaggcggcc gtgcttcctc tgtgaatccc
      361 ttcccgccac agcagtgata cccgcaagat tggagagcaa cattgcaaaa aacattatca
      421 ggatgtgcgg aatcatgatc accgcaggga cattgccctt gaacctgata acaacgggtt
      481 tttccctgag aagcgctgtc tctgaacctg atgcacccct aagtacaacg taatactcca
      541 gcttccctgc agcaggctgg gaggggagtt caccggcaag cttcccgttg tcatgcttca
      601 tcaccacggg tgtccactcg tcattcgtcc tgaaccggcg gaaatacaaa gttcccgaca
      661 cctcctgagg aacctccagt tttatcaggc agggatgggt gttcgtctga ctgcgcttaa
      721 acgcgaagga gtattcggtt ccgtcaacag atgcactggt cctgtatggg taggttggac
      781 cggtcatcct ctggtacatc gcggcagcga tggtaatgat tatggcaatg gtccagaaaa
      841 gaaagttctt catgatgatc gtcgttatgt tgttataact gaattaataa cagttccctg
      901 ttcccgctcc tgaggacttc cacggtcacc ctctggccgg gtttaagctg gttgagcctg
      961 aacatatagt cctcgatatt gctgaccggt ttgtcatcga ttgacacgat gatgtcaccc
     1021 ttctccatac ctcccagcgc cgcgggtttc ccgggagtta caaagtcagc cctcagcccg
     1081 tttttgacat tgccggcaaa gtccggcatg atccccaggg tgactcctct gcggcgaccc
     1141 atcggactcg tcggttcttt gggacctgcc tcggtaaagg ccagtctggc agatctgccg
     1201 gcaacggcat tgactattcc gaacagtaga tcaccggttt tgatcatccc ttcataattg
     1261 agcttgtcag gggtatccat cggggtgtga taatcaagat gggctccggt ggtgattgag
     1321 aggacaggaa tattcctgcc gtagaaggct gagtggtcag acggaccgta cccttcagag
     1381 gtaaaggcga gactcagcgc ggtggtgtca gcatacgcca gcaccgtgtc acgcagcccg
     1441 gcggcagtgc ctatccccgc tacctgcagg ccgttccctt ccttcatccg gcctaccatg
     1501 tcaaggttga tcatcaggtt aacggccgcg ggcgaaatgc cggagttctc aacgaaatgc
     1561 tttgatccca gcagccccat ctcttcaccg gagaaagcca cgaagagaat gcttcttgcc
     1621 ggacgctgct tcatggcggc gagcctttct gcaagctcaa tcataagtgc cacgccggag
     1681 gcattgtcat cagcgccgta atgcacggcg acagtgtcag gtgtcctgct tgacgatccc
     1741 ggtcctccca tgcccagatg gtcatagtga gcacctatta tcacatactc ctcaggagcc
     1801 gatgctcccc tgagcatcat caccacgttt gaagtgacag cctctttctt tattatctgc
     1861 gccctggcgt ctacaatgga agcagtcacc attgaggcgg atgaacccgg cccggccgcc
     1921 ctttcctcaa gtccgttgac ggtgactgaa gtggtggcaa ggatggcatc agccacgttt
     1981 cgttttacct ggaatacggg aatgccggaa gtggactcgt ttcgtgaagg tttgtcaaga
     2041 ttgtctgcaa catcccactt ttctcccgac accagcaaaa cgcctttggc gcctttttcc
     2101 ctggcattaa gtactttaat cctgtcagag ctgagtgacg catagcctgc gtctgatggg
     2161 tttgactcgg ggtaaccacg aagaatcaga gcccatttcc cagacacatc cgttcctgca
     2221 aagtcattgc ggctcagggt atctcctgat ggggtaagtc cgtagccgca gaagatcacg
     2281 ggtgcattaa gctctgaatc cgatgtcagt gcaagcggaa tgaagtcggt acctgcaaga
     2341 tagtctgttc cgttgactga taatctgttg cctgttcctg cttcaaccga aacactcacc
     2401 ctgaagggct gaagtcctga ttcagtaaac ggcccggtgc ccgccttccc gagttctctt
     2461 tttatgaaca ttgcggcaag tgagtcacct ccggttcctg tcccccttcc cgcaaagcgg
     2521 tcagatgcaa gttctccggc gagccatccc agctcgtcag tggtaatggc cgctttcctg
     2581 cccgtgccgg cgcatgcggt catgatcaat gcggtaaata tgatgattcc ggttctcata
     2641 gtctcagatg tcgtgccgac aaaaatagca atcttttaat accaccggat ggtgatattt
     2701 gcaacaaccg aagtaagccg gagtaccccg gagtgaatta aagtgcacct gggcggccag
     2761 aatcagccgg cccgctcagg aatccggaaa ccgcaaaccc ctttctcagg caaacagaag
     2821 gattgccttc tttatgagtg atatggcatc acgcagctgc tcctcggtaa tgacgagggg
     2881 aggagcaaag cggatgatat gtccgtgtgt cggttttgcc agtacaccga gttctttcat
     2941 ggcaaggcat acatcccatg cctccttgcc gttcttcggc ctgatgacga tggcattcag
     3001 cagacctttg ccgcgtacca gctcaatcat gtctgacttc acagccctca tctcgtcgcg
     3061 gaaaatcttt ccgagccttt cagctttttc tgcaagtttc tcctccttaa tgacctccag
     3121 ggcagcaatt ccgaccttgc atgccagcgg attgcctccg aaggtggaac cgtgctcacc
     3181 cggctttatg gtcatcatta tctcatcatc ggccagtacg gcagataccg gaagaacacc
     3241 accggagagt gccttgccca ggatcagaat gtcaggcctg acaccctcat ggtcacatgc
     3301 caggagcttg cccgtacggg ctatgccggt ctggacctcg tctgctatga acagtacatt
     3361 gtgttttttg cagagatcat atgacttttt gatatatccc tcgtcaggaa caaagacacc
     3421 tgcttcgccc tgaatgggct caacaaggaa gcctgcaaca tcagggtcag ccagcgcctt
     3481 ctcaagggca ggaatatcat tgtaggagat ggttatgaaa cccggtgtga acggaccgta
     3541 atccttacgc gcatcagggt cagttgacat ggaaacgatg gtgatggtgc ggccgtggaa
     3601 gttgccttca cagacgatta tctttgcctg gttctccttt attcccttca cgagataagc
     3661 ccatttgcgg cagagcttaa gagcggtctc gtcgccctcg gcgcctgtat tcatcggcag
     3721 gaccttgtca tagccgaaat acttcgtaac atactcctca tactcaccga gcactgaatt
     3781 gtagaatgcc ctggacgtca gggtgagctt tgctgcctgt tcggtcaggg caccgatgat
     3841 cttcgggtgg cagtgcccct ggttaactgc agagtatgcc gagagaaagt catagtaacg
     3901 cttcccttcc acgtcccaga caaatggtcc ttcacctctg tcaagcacta ccggcagggg
     3961 atgatagtta tgcgctccgt acttctcttc tctttcaatg taatcatgag gtttcatagc
     4021 tctctgtttt attttattgt gttgtgttct gatgtgggca atgatacgaa cctttttcga
     4081 caaaaagaaa gagaggaatc agctttcttc ttcggccggc tggtccggtc gcttttgcct
     4141 ggtgaaaaga gaaaatcagc cctcttcttc acccaacaga tccggtcgct tctcccgggt
     4201 gattcggagt gattgttcaa atcgccatct ttctatttca ggagtgttgc caccggtaag
     4261 aatgtcaggc actttccagc ccatgaactc agccggcctg gtgtactgag gcggtgctag
     4321 caatccgtcc tggaaagaat cggagagggc tgattcagca tcggaaatgg ctccgggcaa
     4381 aagcctgacg acagcatcgg tgatgacagc agcaggcagt tcgcctccgg taaggacata
     4441 atcaccgatt gatatctcac gcgttatgag atggtccctg atacgctggt caatgccctt
     4501 gtaatggccg gcaaggatga taatgttctc cttcattgaa agttcgtttg ccattgcctg
     4561 ggtgaatcct gcaccgtcag gggaggtgta tattacctca tcataatgac gctccgattt
     4621 cagacgggta atgcaattgt ataccggctc aatcatcatc accatcccgg cgccgcctcc
     4681 aaacggatag tcgtcaacac tccggtgttt gtcagtggaa tagtctcgca ggtcgatgat
     4741 gtttatctct gcgatgccct tctgacgtgc cctgcggatg attgactcat cgaacggact
     4801 gcgcagaagc ccaggaagta ccgttattat atcaattcgc ataaaaatcc cgtttggtaa
     4861 atctaatatt gttttttcgt atatttgaaa acgacaagtc gaaattaacc atttaaaaaa
     4921 tttagaacat gggaaaattt gttatctcga agaggaaaaa cggtgagttt caattcaatc
     4981 ttcttgcagg caatggccag gtaattctta caagcgaggg ttatgcatca aagccttcat
     5041 gcatgaatgg cgtcaaatca gtcatgaaga atgcaggtga tgtgaagagg tttgaatcaa
     5101 aggttgccaa gaatggcaag ttccacttca accttaaggc aggcaatggc caggtgattg
     5161 gcaagagcga aatgtacgag tcagaaacaa gccgtgaaaa cggtattgcc tcggtgaaga
     5221 agaacgctcc cggtgcaacc attgatgacc agaccaaggc ttaattactt ttgtctttcg
     5281 taggcgattt taccgtcaac gatagtataa tctgatttaa ctgacataat catctcctca
     5341 ggggtattga agatattttc tgagaggatg gttatgtctg ctaaataccc cttcctgatc
     5401 attcccttcc tgtcctccat gaactgggca tatgctgcgc ccagggtgta atattcaatg
     5461 gctttttcca ttgttatctt ctccatcggg aaccagccgt ctccctcttc accctttctg
     5521 tccttgcggg tcaccgatgc gtagagaccc tccatggggt tgaggggttc aacggagtag
     5581 tctgttccga aggccagcct ggccccgctg tctgccaggc tcttccatgc gtaagcccag
     5641 cggctgcgtt cagtgccaat gcgtttctct gcaaaccgtt tgtcagtgat gcaatgggtc
     5701 ggctgcattg acgctatcac tcccaggccc gcaaacctcg gaatatcctc ttcaatgagt
     5761 atctgggcat gctccgagcg gtgcctgctg tcacggatac cgtttacctc ccgggctttc
     5821 tcatatgcat tcagtatcca gttgttggct ttcggaccta tcgcatgtat tcctatctga
     5881 aatccgcgac tgtcggcggc gagcacacgc gcttcaagct cctcgtagct catctggggg
     5941 agaccgctcc tgtcaggtgc atcggcaaac ggctcaaaca tcattgcagt ggctgaaccc
     6001 agcgtgccat ccatgaaatc cttcaggtaa ccaaacctta tccagtcgcc ctgggccggg
     6061 tacttctgcc tcagcgcctc atacctgtca aggcgtgcct ggtcagcggt gagctcgccg
     6121 ccgatgtcaa tccggcacga aagctttccg gcagccatga tcgagtcata catgtcaaaa
     6181 tcggcactgc cgggtatctg tatgcttgtt atgccatatt cggtggccat cttcagcgcc
     6241 agcaggtaac cgctccattc cctctcgcgc tcttcctcag gtgtccgttc gatatctttt
     6301 gcacccgtcc tgatcagtga catggcgctc tccttgagaa tacccgtagg ctccccggtg
     6361 acagggtcat gcatgatctc acccccgaaa ggatcagggg tgcttcgggt aatgcccgat
     6421 tgcctgagca catagctgtt aaccagggca ctgtgaccgt cggtacggcg gaggacaaca
     6481 ggattatccg gtgatacggc atcaatgagc tccttcgaag gccattcgcg ggtggtaaac
     6541 atctcatgct cccagtggcc gccatagatc agctgaccct tctgcgagcg cgccacctgc
     6601 tccttcaccc tgttcgtaat cactgaaggg tcagtgacat agcgaagctc aatataatcg
     6661 ggatcaacag gaccgaagtg cgcatgtgcg tcattaaaac cggggagaac agcccggctg
     6721 cgtgcatcta tcaccttcgt agcctgcgga tcaacatacc tgccgatctc cctgtcactc
     6781 ccgacggcaa gtatcctgtt ccccttcacg gcaacagcct ctgcccgggg attaagcgaa
     6841 tcagccgtaa aaacaatacc tccggtaatg accaggtcag ccttttccgc tttcgtgcat
     6901 cctgacgccg cagtcagtac aagtgcaata atcattaata ttccctgtct catagaaacg
     6961 attttcataa aaccggtgca agataagaaa atagacacta ttttagtcgc tcataaacct
     7021 gtgaacatta actccaatcc tcaaacctat gaaaagcaag gtcgtcccgc ccggttatct
     7081 tttcaccagg cgttattcaa attatgtgtt catcctttta tttctgcttt acctgtttga
     7141 ttatgctgac aggatggtcg tgagctcgat gttctcctac atccaggccg atcttggcat
     7201 aaatgacttt cagtcagggt ggctggtctc aatcatatat cttacaatcg gattgctgac
     7261 ttttccggta tcgcttgtta tagacagatg gagccggacg aagacaatag gcataatggc
     7321 tatcgtctgg agtcttgcaa ccctcctgtg tgcctttacg ggtaattatg tacagctctt
     7381 cctcgcacgg gtactcatcg gtttcggcga agccggatat gccccgggcg gaagcgcact
     7441 catatcagga atgtacccga tggaaaagcg ctcgaagatg atgggactct ggaatgcttc
     7501 cattcctata ggatctgcca tcggggtaac tgccggcgga atcattgcaa gcagctgggg
     7561 ctggaaacac gccttcggcc tggttgccat ccccggtctc ctgattgcaa ttcttttcct
     7621 gttcgtgaaa gattacaaga ccgtagccct ttcaaaggtt gatgagacca acaataagat
     7681 caggatggag aaaaaggaca ttgcaaagga gttcctgaag aaaccctcgg tcatatttac
     7741 atatttcgcg atgacggcaa ttgtgttcgt gaccacggca ctcctggtgt ggctgccgaa
     7801 attcttccag gttacaagcg gaatggatcc gaaaaaggcc ggaagccttg ccggtgcggt
     7861 tatgatgctg gccctggtag gagcacccct gggcggctat ataattgacc gctggagaaa
     7921 gactatgcct acggcacgaa tgatctatcc ggccatctct acactactct cagcactcct
     7981 gctgttcctt gcactctacg tgttcagggg tacgttgcaa ctggtgacac tcttcatatt
     8041 cggaatattc ataatgcagt tcataagcgg cgcagctgct gtcacacagg atgttattca
     8101 cccgggactg cgggcaacat catatgcaat agcagttctc gtgcagaacc tcctcggctc
     8161 atttacagca cccattgccc tggggagaat ctctgacctg actaacatac agacagcagt
     8221 aagcattctt cctttttctc ttctgctggg cggactgcta ttcctgattg gggcgcgcta
     8281 ctatataaag gatctgaaga aggtagcccc agttaccctg gaggcagaat aaaccggttg
     8341 aaaataccac tcatgcctgt tacgtcactc attccctgct gacaagatga caagggcagg
     8401 tgcatccgtt cacagcaggg cttattgagg tgtttccgct taaaggaatg ctgtattcct
     8461 gcctgcccga atcaccatgt ttcaattgcc ggtgtacccg cctcacaggt cccatcagaa
     8521 agaggtatta ttcgcacagg aaactgattt gaatttatta tagtagattt gcacatcgaa
     8581 aaccaaagaa gatgaaagaa cttttgcgga acaaattttt caaattcggc atagccggag
     8641 tactctacct cctgtgggtg atatgggtgg gcaacttctg gctgctgctc ggattgccga
     8701 tagtattcga catatatgta tccaaaaagg taaactgggc attctggaag aaacgccacg
     8761 gtaaaaatca tgttgttatt gaatggcttg atgcactgat ctttgcggtg attgcagtga
     8821 cattgatcaa tatcttcctc ttccagaact accgcattcc gactccctcc atggagaaga
     8881 ccctgctggt gggtgatcac ctggctgtaa gcaaggtggc atacggcccc cgcatgccca
     8941 atacccccct cgccttcccc tttacccagc ataccatgcc tttcaccggc ggcaagggga
     9001 aatcatggtc tgaccttatt cactggaagt ataaaagact caagggattc gggcatgtca
     9061 aaaacaatga tatcgtcgtc tttaacttcc cggctggaga tacagtctgc ctggagcagc
     9121 aggcaataag ctactatgag attgttcgtg aacggattga gacactcact gcggaggata
     9181 tcagggctgg caggcctttg aaaagcaagg atgagtatta ttcagttgca aggcagatga
     9241 tctggaatga gtacacggta atctacaggc ctgttgaccg gcgtgacaat tatgtgaaga
     9301 ggtgtgttgg catacccggc gacactgtcc tgatcaaagg agggaagctg ttcatcaacg
     9361 gtcatgagaa cgcggagttc gagacccagc agacatccta ttacgtgcgt accaatggta
     9421 cccggataaa tcccaaggct tttgaaagaa tgggcgtgtc aaaatcagac cagtccctta
     9481 tgctttcagg aactgtctac aggctgccgc tgacgaagga aaacgcaaag acaatctcag
     9541 gatttgcaaa tgtcaccggg attgaggccg actaccacag gcccggtgaa tatcttgcct
     9601 ctgtctttcc tcatgatccc gcgtaccgct ggaacgagga ttacttcggc ccgctctgga
     9661 taccgaaaaa gggaaccacg gtgaagcttg acatgaccaa cattaacttc tatacatcca
     9721 ttatcgatat atacgaggag aatgacctgg agatacgtga tacagtaata tatattaacg
     9781 gcaagcctgc tgacagctat acctttaaga tggattacta ctggatgatg ggtgataaca
     9841 ggcatgactc agccgactca cgcttctggg gattcgtccc tgaggatcac atcgtcgggc
     9901 gccctgtgcg tgtctggctc tcaattgaca aggaagccca gggatttaaa aagataagat
     9961 tcaacaggtt cttcaaggca gccaagagat aattgcgaat tgcgatttga gatgttcgat
    10021 ttaattcgca aatcacaaat cgcaaatcgc aaataaaaaa gcgggcctca gcccgctttt
    10081 ttttactctt ttactttgtc cactatcgcc ttaaaggctt caggatggtt catcgccagg
    10141 tcagcgaggg tcttgcggtt aagggaaatt cccttcttct caagcccgcc gataaactgc
    10201 gagtaactca ttccgtgaag acgtactccc gcgttgattc tctggatcca gagtgcgcgg
    10261 aactgcccct tcttgtcctt gcggtcacgg tacgaatagg caagcccttt ctcaagcacg
    10321 ttctttgcaa cgctgtaaac attggcgttg ttgccgaagt accctttggt ctgcttgaag
    10381 atcttttttc ttcttgcctt cgatgctggt gcgtttgttg ctcttggcat ttttgttctc
    10441 tttttcggat gtaagcgcct ctttaaggtc ttttatgctt cacatcccgg ttaataattc
    10501 tgttgattaa ctctccgtca ggcggtaaac actacttcag cccgagcaga agttttacgt
    10561 tcttctgatc tgccttgtca accagtccgt cataggtaag attcctctta cgctttgttg
    10621 acttcttggt aagaatgtga ctcttgaagg catgcttacg cttgatttta cctgaaccgg
    10681 tcaaagaaaa tctcttcttt gcacccggat ttgttttcac ttttggcatg ttgtatatat
    10741 atgtgtaaaa aaaatctatt tctttttctt tggcgagagg atgattatca ttctctttcc
    10801 ttccagtctc ggagtctggt ctacctttcc gtactcatcc aggtcactca caaatctcgc
    10861 aagaagcttt tctccctggt ccttgaagag aattgacctt cccttgaaaa agacatatgc
    10921 ccttactttg gccccttctt ccaggaagtt gatagcgtgc ttcagcttga agttgtaatc
    10981 gtgatcatcg gtgttcgggc cgaaacgaat ctcttttaca acaatcttgg ctgcattggc
    11041 tttgatctcc ttctgcttct tcttttgctg gtaaagaaac ttctggtaat caactatctt
    11101 gcagacgggt gggtcagcat ttggagatat ctcaacaagg tcaagctcca tttcgtctgc
    11161 cagcctgaga gcatcagaaa ggttcatgac ttttgcttct atgttctcac cgacgactct
    11221 taccgtcctg gctgtgatcc tgttgttaat ccggtgttcc ggttcgggtt ttcttcccgg
    11281 cctgggcctg gaaaagatcg gtcctgctat tgttttgtcc tccagttaag ttaataatat
    11341 gtttaaaatc tgtattttaa ataccagtct ctctctttat ctcctctttc acaaggttga
    11401 tgaactcccc gggtttcatg actcccctgt caccttctcc ctgcttcctg acggagactg
    11461 ttcccgctaa ttcttccttt tcaccaatca caagaagata gggaattctc tttaactcat
    11521 tatcccttat ctttttacct atcttctcgt tcctctcgtc aatcagggtg cgaatatcgt
    11581 aattattaag gagatttgaa acagtttttg cataatcatt gaatttttcg ctgatgggca
    11641 gtattacagc ctgatcagga gtcagccaga cagggaaacg gccggcagta ttttcaatga
    11701 gcactgcaac gaaacgttcc agggaaccga aaattgccct gtgaatcatc accggctggt
    11761 gcttctggtt gtcgctgccg gtgtactcaa gcccgaaccg ctccgggagg ttgtagtcta
    11821 cctggatggt cccgagctgc cacttgcggc cgatggcgtc acgcaccatg aaatcgagct
    11881 tgggtccgta aaaggctgcc tcacctattg ccacagtggt cacaaggttc ttttcccctg
    11941 cagcttcaat gattgccttc tcggccttct cccagttctc gtcagtaccg atgtacttat
    12001 catggttacc cggatccctg agtgatatct gtgcggtgaa ttcattgaat ccgactatcc
    12061 tgtaaatata ttgcacaagg tcaatgatgc tcttgaactc ctccttgagc tgttcagggg
    12121 cgcagtaatg gtgagcgtca tcctgggtga agctgcgcac gcgggtcagc ccgtgcagct
    12181 caccgctctg ctcatagcgg taaacggtgc caaactcggc catcctcacc ggaagctccc
    12241 ggtaggagtg gggagtggcg ttgaatatct cgcagtgatg agggcagttc attggcttga
    12301 gcatgaagac ctcaccctcc tgcggagtgg tgatgggcct gaatgagtca gccccgtact
    12361 tctcaaagtg acctgatgtc ttatacaggt caacattgcc gatgtgcgga gtggatacca
    12421 tggtgtaacc tctcacctta agtacctttg tcatgaaatc gataagcctc tgcttcaggt
    12481 ccgctcctcg cggaagccag agagccagcc ccattcccac cttcggcgag aaagcgaaca
    12541 gctccagctc cttgcctatt ctgcggtgat cacgcttgcg tgcctcttcg atcattgtca
    12601 tgtactcatc aagcatcttc tgcttcggga acgtgatgcc gtaaatgcgg gtcatcatct
    12661 tccgcttttc gtcgccacgc cagtatgcac cggcaaggct tagaaccttt atcgccctga
    12721 ttgatgaagt gtccggaagg tgcggaccac ggcacagatc ggtgaaattt ccctgccggt
    12781 acagagtaat tgtaccgtct tcaagctcag agatcagctc ggtcttgtat tcatcaccct
    12841 ttgcagtgaa gagctccatc gcttccttct tgctgacctc cgtgcgaact atcgggctct
    12901 tccttgctgc aagctccttc atcttttctt caatgagggt caggtcggaa tcggttatca
    12961 cctttccgtc acctggatca acatcgtagt aaaagccgtt ctctatggcc gggcctatgc
    13021 cgaatttaat cccggggtaa agtgcctcga gtgcttccgc cataaggtgg gctgacgaat
    13081 gccagaatgc atgcttcgcc tcggcatcat cccacttgtg aaacctgaca gtggcattgc
    13141 cttcaacagg ccgcgaaagg tcacgaacct caccgttgac cgttgctgca tagacatcag
    13201 cggcaagacc cggactgatt gacattgcaa tttcatgact ggttataccc tggctgtact
    13261 ccctcacaga gccgtcagga aaggttatgc tgatcatcgg tttcatactt tttaatagtc
    13321 cgcaaagata atatttttta ctgtatggtg ttctcctgtg ttttcgaggt aaaacggccc
    13381 tcttttttga aattgttaat tggcacagat tttggattta acaaaacagc accaaaaacg
    13441 gcagtcatga aaagaatttt aatcttaacc gcagcagcag cattgataat gctgaattca
    13501 tgtgacacac agcccgaagc ttacttcttc gcagataaga taaaggctga aataggagag
    13561 gaggtattct tcttcaacgg gtcatacaac gcaactgatt acgaatggga ctttggcgac
    13621 ggcacttttt ccaacgccta cgaacctgcc cacgtctaca acgcatccgg ggtatttaca
    13681 gtggtcctga ccgcatatac gagatcgggc aatgtcgaca agtcttacct tgatatcgag
    13741 atcaactccc cgacaatgct tgaggttgag gtgcttgaat actacgacca gtatccggtt
    13801 tcaggggcaa gcgtgatact ctatcctacg gagacagact gggacaatga gaccaatgcc
    13861 atagtggagg ggttcaccaa cgcctccgga aaggtcatat tcaccaacct gcagcccagg
    13921 gtctactttg tcgatgtctg gcattcaacc cacaacaact acaccctgag aaacgaggat
    13981 acgggcttca tcaggacaga tcagctcgaa aagaatcagc tcaaccagtt cactgcatgg
    14041 gttgactaca caggcaccaa gggagctgca atccgcgaca ggaaacagct tgtcccgctc
    14101 aagagcagga cggtttcagc aaccgtaaaa aagtaattca caggggtaaa caaaatagta
    14161 tggcgcacga atccatttga taaatttctt taagtttgtg tttcacaaac ctaatcgaaa
    14221 gaatctcatg gatacaaaat ttgcgcttca tggaatagac taccttataa tggcagtcta
    14281 tttcgttttt gtgctgggta taggttgggc cctgaagaag tatatgaaga acgccactgc
    14341 cttccttgag gcgggaagat ccatccccgc ctgggttgca ggcctggcat tcatctccac
    14401 gaacctcggt gccctcgagg tgatgggcat gactggctca ggtctcaagt acggtatgct
    14461 caccacccat ttctactgga ttggcgccat accggcaatg gtcttcctgg ccctcttcat
    14521 gatgcgtttc tattacggct ccaaggcacg gtcagtaccc gagtacctga agcttcggtt
    14581 tgatgagaag acgcgcggac tgaatgccat cctgtttgct gttatgacag tgcttgcctc
    14641 ggggatctcc atgtacgccc tcgcccttct ccttgaaaat gtactgggat ggaacttcaa
    14701 cgtatcactc ttcgcctctg cggcgatagt gctgggttac acataccttg gaggactctc
    14761 ctctgccatc tataacgaag tgcttcagtt tttcattatt gtgataggcc ttctgccaat
    14821 ggctttcctt gtcatgaagg atgtcggggg atggggcggc atgcaggcca gcctggcccc
    14881 gattgtggag agcaagggtt ttgcggctga tgcgtggact acaacctgga agtatacagg
    14941 aaccggctcc ggaaaccctc tgggagtggc atggtacggc atcttcgcag gattgggctt
    15001 tgtgctctcc ttcggctact ggtgcacaaa cttcctgatt gtgcagcgtg ccatggcggc
    15061 cgagtcaatg acggctgcac gcaaggtgcc tctgatcggc gccataccta agatgttcat
    15121 tcccttcctt attattgtcc ccggcatcgc tgcacttgtg ctgctgaacg atccgctgaa
    15181 aggatttgca ctgccattga acgacgccgg gcagcctatc tatgactata ctataatcat
    15241 gctgctcaaa cagtacctgc ccgccggcgt acttggtctc ggggtgacgg cactgctcgc
    15301 ctctttcatg tcaggcatgg ccggaaatgt ttcggctttc aatacagtat ggacctatga
    15361 catctatcag agctacatcc ggccggcaac aggcaacaag gaggctgatg acaaacatta
    15421 tctcaaggtc ggaaagatgg ctaccgttgt gggagtgctt gtcagcatcg ctgcagcgta
    15481 tattgcaagc aagttcggca acataatgga cttcctccaa acgatcttct caatgatcaa
    15541 tgccccgctc tttgcggtga tattcctggg gatgttctgg aaacggtcaa caggcaatgc
    15601 ggcattcaca ggactgctgg caggattcct gatggctctg gtacatcatg gtttgactca
    15661 gccagaggga gccaccaccc tcgtcaaggg cggatggctc ggtgtagtcc atacataccc
    15721 tgttgagatg gcacagaact tctggacagc aattatagcc tttactgtgg ctacgctgct
    15781 gacggttttt atttcgctgc tctccaaacg caccaagacc gatgatgaac tcgacggcct
    15841 tgtctactca atgacgaaga agctcgacga ctccggagtg gtgtggtaca acaggccctg
    15901 gttccttgca cttattgtgg gagttattat gattgtgctc tcaatcttgt tctggtaact
    15961 ttaaaaacga cgatcatgtt agatataaga tttccgattg gactgatgtt ctcgattttc
    16021 gggctgatca ttacaatcta cggcattgcc acactgggta atgatgcaat gtatgcccgt
    16081 tcgctgaaca taaatgtaaa cctggtatca ggtatagtca ccctgctttt tggactcatc
    16141 atgctatttt tctccggccc ggtgaagaag ctgctgaaga gaaaatgaca aaaaaaaagc
    16201 tgctgagagg cagctttttt tatttgcgaa tgtcgatttg cgaattgcga atcgtggaaa
    16261 aatcgcaact cgtaacatgc aaattacaaa tcactgcagt tcctcggaaa acagtgccat
    16321 gggcagagat cgcatattgt acctcgaggc catcacctca ccgtatgcac cggcagagcg
    16381 aatggcaacc aggtcgccgc gtcgcgtttc cggaagcgaa atactcctgc cgaaggtgtc
    16441 agtggtctcg cagattggtc ccacaacagt atattttttc accggaccgg aagaggagag
    16501 attctcaatc ctgtgactgg catggtaaag ggcaggcctg ataagctcgg tcatgccggc
    16561 atccaggatg acaaatgtct ccgcagcgcc gggcttgaca aacagtaccc tgcttacaag
    16621 agagccgcag ttcgccgtaa gcgaccgacc cagttcaaag gatatggtgc tttgtatcct
    16681 gctgtcaaga tgcttcctga aaacgtcaaa atactccgcg aactgcggga aacggtccgg
    16741 gtcatcatag ctgattccca gtcctccgcc tacatttatg tcttcaggcc cgaagcccct
    16801 gtcggtcagc cactgccaca actcgtttat ccttgtgcag agattgcgat agacatgcag
    16861 gtcggtgatc tgtgatccga tatgaaagtg aattcccctg taacgaaggt tctgcagccc
    16921 ctccagcctg tccagaacag tttcaagctc cgggattctt ataccgaact tgttttcatc
    16981 ggtgccggtg gtgatatgct tgatggtgtg tgcttccacg ttggggttga tgcggagtgc
    17041 caccggggcc tttttcccca gcctgcctgc aatagcgtcg atgacatcga gttccgctgc
    17101 ggattctgca ttgaaacact tgatgtccag cctcagagcc ttctctatct cagcgtcgct
    17161 cttgccaacc cctgcaaaca cagtctcgcc ggccggaaat ccgcatgcaa tggcatgctc
    17221 cacctcgttg ccgctgacac agtcagctcc gaatccgtac cctgcgatga tcttcataat
    17281 cacaggattg aagttggcct ttacggcata gtgaacatga aaacccctgt tgacagcttc
    17341 acctgatgcc acggagagag tctcacggag catttccaga tcatagtaat aaaaaggggt
    17401 ttcatgctta cccagttttt cagtaatctc cctggtaatc atgctttctg tgtaaataac
    17461 ctttcattca gcgccctgag ggctgtgatc ttgtcggctg tgttgataag gatcgagacg
    17521 ctgtgatcac tgccgccgta ggagatcatc tttaccggta tctcgttaag cgcttcaaat
    17581 atctccgggg ccgatcccac acggtcaggg cggaagtcgc ccaccacaca tataatggtc
    17641 tggttgcggt ctgtctccac ctgcccgatt gaactgagcg atttcacaat gtcttcaagg
    17701 tgggcaggat cctctatggt cagtgataat gagacttcgg aggtggttat catgtcaata
    17761 ggagtcctga aggcttcgaa tacctcgaag atcctcctca tgaaaccgta agccatcagc
    17821 atcctgtcag agtgaatgcg tatcgaagcg atgccgtctt ttgcagccac tgcagtataa
    17881 tcttccaggg tggtttctga ggtgataagg gtaccatcat cggcaggttc catcgtgttc
    17941 ttcagccgta ccggaatatt tttcatcctc gcggggttga tgcttgaggg gtgaagtatc
    18001 ttcgccccga aaaaagccag ttctgccgct tcgctgaagg agagatttct gataaccctg
    18061 gtgttctcca catacctcgg gtcattgttg tgcagcccgc tgatgtcagt ccatatctgt
    18121 acctcttcag cggctatggc agcccctatg attgtggcgc tgtagtcact tccgcctcgg
    18181 cggaggttgt cagtctcgcc gaagacgttg cggcagataa agccctgggt gataat
//
LOCUS       DAOPXU010000055        18223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_3213_length_18223_cov_11.743340, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000055 DAOPXU010000000
VERSION     DAOPXU010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18223
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3213_length_18223_cov_11.743340"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..378
                     /locus_tag="PKK03_07095"
     CDS             37..378
                     /locus_tag="PKK03_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX84205.1"
                     /translation="MSDIVFLLLLFFMLTSTLVQPTALKLLLPKGTTQSSARPMTTVS
                     ITKDLQYFIEDQPIDLEFLEAALQSRVQDSPEIYISVHADRSVPVEYVVNVMNIAAKN
                     QYKLILATTPK"
     gene            359..1249
                     /locus_tag="PKK03_07100"
     CDS             359..1249
                     /locus_tag="PKK03_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNX84206.1"
                     /translation="MPQLRNSSGEPLTKERKQGLIFSALTHLLLAILIIFVTFRVKIP
                     EPVEEGLLVNFGFDETGEGLFEPAPEPVSPPPPPESAGEQGGEEALLTQDFEEAVEVE
                     KKEPSPEEIRKQAEARAAEIRRQLEAEAERKRIEQEEAEKRKKEEEQRRIDQQNARAR
                     GAFSNAGNVGTSGQSQGVAGGQGNQGVQSGTPDAPNYGPGGGQGTEGISFDLGGRKAQ
                     SLIKPPYDLQKEGVVVVAITVDRSGRVTDATPGIKGSTTLEGDLLKLAKEAALKTRFE
                     SSNDAPIIQKGTITYVFRLK"
     gene            complement(1262..2035)
                     /locus_tag="PKK03_07105"
     CDS             complement(1262..2035)
                     /locus_tag="PKK03_07105"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNX84207.1"
                     /translation="MEWYMYLAVIAAGLFAGFVNTLAGSGSLITLPLLMFLGLPANIA
                     NGTNRIGVFVQSLVSSISFKKQKLYTVGESLWMSVPAIAGALLGSLIAVKINERMMEL
                     LIGGLLVFMFFVILYKPEQWVRDHAHSPAMKRRWWVPVVFFIIGIYGGFIQAGVGFFL
                     LAALVLGTGMGLTRANAHKVLIVAAMTAIALVVFILSNQVYYLYGIILAVGQGAGAWL
                     GSKVAVSWGPRVVRVILLVAIMASAIKLTGLYDLVAGLF"
     gene            complement(2048..2446)
                     /locus_tag="PKK03_07110"
     CDS             complement(2048..2446)
                     /locus_tag="PKK03_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX84208.1"
                     /translation="MEKVSPQTDILVYVVDDDPVVTSLVSTRLEGNGYAVRAFAYAED
                     CIAALGEIPDLIILDYYFLKEGEIPMNGMEAYDEITRLAPEVPVIMLSAQEKGEVVLE
                     FARKGIADYVIKDSDLIDNLLMAVKEVLER"
     gene            complement(2480..3760)
                     /gene="glyA"
                     /locus_tag="PKK03_07115"
     CDS             complement(2480..3760)
                     /gene="glyA"
                     /locus_tag="PKK03_07115"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNX84209.1"
                     /translation="MERDSAIFELIDRERKRQTEGIELIASENFVSPQVLEAMGSVLT
                     NKYAEGYPGARYYGGCEVVDEVEQLAIDRLKKLFNAEYANVQPHSGAQANMAVFLTIL
                     KPGDTFLGLNLSHGGHLSHGSPVNSSGILYNPVAYGVKEETGRVDYDMMEEVALREKP
                     KLIVGGASAYSREWDYARMRAIADKAGALLMIDMAHPAGLIAAGLLDNPLRYAHVVTS
                     TTHKTLRGPRGGIILLGTDFPNPWGVTTPKGEIKMMSAMLNSAVFPGIQGGPLEHIIA
                     AKAVSFGEALKPEFRTYQQQVKKNAAVMAEAFMAQGYKVISGGTDNHSMLIDLRTRFP
                     EISGKKVENTLVRAHITVNKNMVPFDSRSPFLTSGIRVGTPAVTTRGLKEGDMGTIVQ
                     LLDEVLVNIENESVITSVGEKVVKMMKDLPLFAY"
     gene            complement(3914..5686)
                     /locus_tag="PKK03_07120"
     CDS             complement(3914..5686)
                     /locus_tag="PKK03_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNX84210.1"
                     /translation="MNNLIIRKIRQLKSFLTNQQTLIYILSFLVGLVSALAAVVMKNA
                     IHFTHVLFTQGVMKGAGGVLSLAYPLAGIFLTMLAVKYLVRDNISHGISRVLYAISKR
                     KSYIKPHNNWSSILTSSLTIGFGGSVGAEAPIVLTGASIGSNIARHFKLNYKYVTLLL
                     GCGVAGAISGIFKAPFGGILFTLEVLMLDLTMSSIVPLLISSVTAATVAFFFMGDNVL
                     FSFQVKQGFMLSELPYYLILGVFCGLVGIYFTRATIGIEKLYKRIGNKWIRMVAGGSI
                     LGALIYFYPALYGEGYETVLVLLNAGSGVAPGNEIFGNMAAGFWSFSLLMAGLLLVKV
                     VATASTNGAGGVGGIVAPVLFTGGVAGYYLSSLLNHLFGLALIESSFTLTAMAGVFSA
                     VLHAPLTAIFLIAEITEGYALLIPLILTSTVAFITARTFQKYSIYNEQLAITGELITH
                     DKDKAVMTLLDWSREVEKDLLTVRPDSNLGDLVKQISKSNRNLFPVVDEFNILEGVVL
                     LDDVRDKMFRPELYDKITVRELMTIPPSYIDLSERGDAVLDAFERTGAWNLPVLDEGR
                     YVGFISKSRIFSAYRELLRQFSEE"
     gene            5783..7024
                     /locus_tag="PKK03_07125"
     CDS             5783..7024
                     /locus_tag="PKK03_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009040279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNX84211.1"
                     /translation="MIGFERQILDNGLRVIVHRDEGTPMVTTNILYDAGSRDEEPDST
                     GFAHLFEHLMFEGTQNIPLFDVPVMMAGGENNAFTNNDLTNYYITLPATNLETALWLE
                     SDRMRGLDLAEERLANQKSVVTEEFRQRYLNQPYGDLLLLLRPLAYRVHPYRWPAIGM
                     DISHIEKADSISAKKFYDRFYTPSNAVLSVAGNVDPDRVFSLAEKWFGDIPSGVKTER
                     KLPVEPEQAERRELTVHRPVHAPHLLMAFHMGSRESRDFYILDLLTDILAGCDSARFP
                     AILVRKKELFSEADIYLSGEADPGLVIFSGRIRDGIDVRKAEEAVRSELDRLASERVI
                     AAELDKARNRYESHRLMQYISVSNKAFSLAYHEVLGDADGINREHEQYMSVTAEEIAS
                     AAGLTFRPGNSSVIHYLPEKI"
     gene            7021..8307
                     /locus_tag="PKK03_07130"
     CDS             7021..8307
                     /locus_tag="PKK03_07130"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNX84212.1"
                     /translation="MRNKEIKGAPEIITPSAGDTSLPEPVMLGNGVPVYMAGNGTVDL
                     LRIEFVIPAGQVLEEVHLAASATSAMLTEGTLHHDAATINDLIDSTGAALTHSSDKDT
                     AGLVTVTLTRKLDDVMALASEVLFSPSFPENEFRMLTDRRLQAFQTSRRRTSVIAREA
                     FYEALCGSRSPYGRITTEDDYRRLTTGDVREFHRKHYRPVNMYVTVAGSEPWKALPVL
                     EKYFSGKGTGQWQKPPLPSPVLETSEPGKIFRDLPTSVQSTVRVGWKGITRNHPDYQA
                     LQVATMILGGYFGSRLMRTIREEKGYTYGIGAVAGAFHNIGYITIMTDVANAYRDETL
                     SEIRKEIEKLRTEGVSEEEMTLVRNHMMGELARIFDGPFSVADAIRGIIDYETGADYY
                     TRLAETVKTITPARIMDIFNRYFNPGEAWEIIAGSR"
     gene            8304..9779
                     /locus_tag="PKK03_07135"
     CDS             8304..9779
                     /locus_tag="PKK03_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNX84213.1"
                     /translation="MKKQNTLRKLFIPLLFCGLSAFPLAGQDADRPLSPRLDMVTVDP
                     ATGFAVLRWLPSPSPDVGSYVVYIYAAGRADAIDTIYSPFVFEYTHTASAARYRSVSY
                     VVAAIDSSDNPSPLSNSLSTIWLSAAEDPCTATVAVSWTAYDNQFQPGSGYLLHITDG
                     TGGSFPDIALPVSSTQYLFTGYEADTEYCFYVTASDGYGPLSSSNRACVTTGIEAAPG
                     WVRIDAIAVQDGGISFAAGYDPSTVMSDFRLFRYDPVAAAWDKAASSTGVAGRVTFAM
                     AQADTTVVRLYRVAAVNSCGLASTVSAPSRNILLEASISGTLVNLRWNRPSAGWSGSF
                     SVWRDIGEGMREVASSMSDTLWSDDYTGFASDVSAGEVVYRVSTADLSAPAGAGPHLS
                     SAAVIATSENVFMPNAFTPGGTDENAIFRPEFSFHPGEYDFRILSRNGALLFRTGDPG
                     EGWDGRHNGRMMPPGVYLWNLRLTTPSGQTLERNGTVTILP"
     gene            9841..12102
                     /locus_tag="PKK03_07140"
     CDS             9841..12102
                     /locus_tag="PKK03_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNX84214.1"
                     /translation="MVKTRTKIFTADDISRIETEYSLLTDNLYRCDKPGDRELIEKAF
                     RFANEAHRDMYRNSGEPYIIHPINVARIVNQEIGLGAKSVSAALLHDVVEDTGIPLEE
                     ISKHFGPKIASLIDGLTKISGTYNKETNSLQAENFRKMLMTLSDDFRVILVKIADRLH
                     NMRTLDSMPEYKRMKIAGETVYLYAPLAHRLGLFAIKTELEDLSFKFRHPRIYDEIAR
                     KVKMDEKKNLALINRFSLPIIEALNNNNILFDISGRFKSIYSIWKKMQSKNVPFEEVY
                     DLLAVRIVFEPGPEMSERAQCWQIYSLITDIYTPKPDRLRDWISRPKSNGYEALHLTV
                     MGPEGRWVEVQIRSQRMDDIAERGFAAHWKYKGDMSQTENELDKWLKHIREMLANPMS
                     DPLTFLDEFKMSLFSSEIMVFTPKGLLVNLPKGASALDFAYEIHTDIGNKSIGAKVNY
                     KLSPLSTTLQSGDQVEIITSNIARPEKEWLEYAHTAKARNAIKDALRDESTDRIQKGM
                     DMLEQKLSELGILPNSDIIKKLLDNYELLNKDELYSRIGLGLINLDELRKVLKKAPEG
                     SLRKYWKLAIGGVTRKNPETREAPVTAEKIDKSQPYLLRENVDSAASSYVIASCCQPI
                     PGDEVVGYLDHSNSIIVHKPKCPVAVKLMSSEGNRIIPVRWTIHKLASFLARISINGI
                     DRIGLVSEVTTIISKELNVNIRNINVSVYDGIFEGTIDLFVHHTRDLNNLIMQLSNIK
                     GIASVKRIEEFIE"
     gene            12129..12575
                     /locus_tag="PKK03_07145"
     CDS             12129..12575
                     /locus_tag="PKK03_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNX84215.1"
                     /translation="MSNEEARAVVRQVFTEYLATHSLRKTPERYAILDEIYSREGHFD
                     IESLFKFMNNKNYRVSRATLYNTIDLLLDCKLVTRHKFGHNQAQYEKTYKAGHHDHLI
                     DTISGKVIEFSDPRINDIIRSACEENSFVVHHYALYVFGVQEKRGG"
     gene            12645..13907
                     /locus_tag="PKK03_07150"
     CDS             12645..13907
                     /locus_tag="PKK03_07150"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNX84216.1"
                     /translation="MNIDILLGLQWGDEGKGKIVDVLTPDYDIVARFQGGPNAGHTIE
                     FHGVKHVLHLIPSGIFHDDKINIIGNGLVIDPVVFRHEVESLGIDIGKVRERLLISLK
                     AHLIMPTHKLLDAAHEASLGEAKIGSTLRGIGPAYTDKASRNGLRVGDLFRKDFREFY
                     HSRVNDHLRTLQGYGYTSDLKALEDEWFDAVEYLKQFRTVNADYHIDQALRAGKRVLA
                     EGAQGTMLDIDFGTYPYVTSSNTVAAGACTGLGIAPGRTGEVFGIFKAYCTRVGSGPF
                     PTELDDADGQQMRDQGNEYGATTGRPRRCGWLDLPALKYAIMVNGVTRLFMMKADVLS
                     GFSEIKVCNSYAVDGREQAELPYCLYDISGLNYRTFRGWHCDITGCRSWEELPQELKD
                     YIRYIEDETGVPVTIVSVGPDRSATITR"
     gene            complement(13911..14378)
                     /locus_tag="PKK03_07155"
     CDS             complement(13911..14378)
                     /locus_tag="PKK03_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84217.1"
                     /translation="MKKLSLVLLAAVLVLTPSCDFMKSINPFGKKAREAEALRQQQEA
                     FRIADSIRVANEKQAEAERARQAEMAVAAEEQTRVMTGYHVIVGSFLTPEYADAWLEH
                     IKSFGYDAQLVGMNGGRWQLVSAQAFPNLHQAWNALGTVQDRINGEAWVYRQE"
     gene            14521..16434
                     /locus_tag="PKK03_07160"
     CDS             14521..16434
                     /locus_tag="PKK03_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX84218.1"
                     /translation="MKSLIRFFEENVEKYASNIYMWEKLQEKYEGTTYQETRRQVHEF
                     GAGLMQLGIKKGDRVSLLADGRNSWVVGELGILYAGGVNVPLSIKLNPEEIKFRLIHS
                     GSRMVITSSTQAPKILAVAEKCPDLQKIIFMDPREEYAEGQIHFNEVRKIGREWLENP
                     ANRKSFEEFTSSITASDFANICYTSGTTADPKGIILTHGNYVTNVYQSYSLMDIPQFY
                     KTLLILPWDHSFAHTAGIYAFMGKGASIASVKVGKTPMETLRNIPVCMKEIKPNLLMS
                     VPALAKNFRKNIEKGIKEKGRMTEALFNFAIKMAYSYNKEGWNKGRGIQKIKKPLLRL
                     FDKILFSKVREAFGGELEFFIGGGALLDIELQRFFYAIGVPMYQGYGLSEASPIISSN
                     STARHKLGSSGYLVNNMELKICDDNGNELPVGQKGEIVIRGGNVMHGYWKNDTATSDA
                     LRDGWLYTGDMGYMSEDGFLYVLGRFKSLLIADDGEKFSPEGIEEAISEQSKYIDQCM
                     LYNNQKPYTVALIVPNQHAMKLYLEDRNMSATTEEGKRAVLNLIENEVNEYRINGKYG
                     HMFPHRWLPVAMGVLNESFNEENGMMNSTMKIVRGKITDRYHDLIEWLYTPMGKIVYN
                     ERNMEEVEKMKLG"
     gene            16455..>18223
                     /locus_tag="PKK03_07165"
     CDS             16455..>18223
                     /locus_tag="PKK03_07165"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNX84219.1"
                     /translation="MKYLLIPVLVVIAGMMTSCNREKTPEWKPAENPIFTVWGEKMDP
                     SAPWPEYPRPALARDEWMNLNGLWDYAICPKDSDRPEPQGKILVPFPVESALSGVRER
                     MSDSLALWYSREFVLPKQWKDKRIIINFEASDWETTVWVNDRMASMHRGGYDPFWCDI
                     TNYIDDRKKHTLVVKVTDPTDRGPQPRGKQVTKPGGIWYTPTSGIWQTVWLEPVEKTW
                     IGDLRVVPDIDNGVLHVTVIEEHCQGGPGGCPPVDMAPSLAEITLLAEGKTVAVASGI
                     TGSVVTLPVPDARLWSPDDPYLYDLKVRLVTGGLVADQVTSYAGMRKISLGVGEDGFT
                     RIFLNNEFMWQNGPLDQGFWPDGLYTPPSDEAMVYDLEMLKKMGFNMLRKHVKVENRR
                     FYYHTDRMGLLVWQDMPSGDDYIWGDKPDIEKSASDSAVFMDELTMMIGTKFNHPSIV
                     MWVVYNEGWGQYNTPAVTDYVMALDTTRLVNSASGWTDRGTGHVLDIHHYPDPVAPGP
                     EKDRAIVLGEFGGLGLPVQGHTWEQKNWGYEKMGDTTALLNKYTEFYAAVRQMVAEKG
                     LSASVYTQTTDVETETNGLMTYDR"
BASE COUNT         4637 a         4502 c         4929 g         4155 t
ORIGIN      
        1 aagaataaag tcagtgttca gttcagcaat gcaggcatgt ctgatattgt gttcctgctt
       61 ctgctgttct tcatgctgac ctccaccctg gttcagccga cagccctgaa gctgctgtta
      121 cccaagggta cgacccagtc ctcggcccgt ccgatgacca cggtatcaat tacaaaggat
      181 cttcagtatt tcatcgaaga tcagcccatc gatcttgaat tcctggaggc tgcgttgcag
      241 tcgagggttc aggacagtcc cgagatatac atttcggtgc atgccgacag gagtgttcct
      301 gtagagtatg tagtcaacgt gatgaacata gctgcaaaaa accagtacaa gctgatactt
      361 gccacaactc cgaaatagtt ccggtgagcc tctcacaaag gaaaggaagc agggtctgat
      421 cttcagtgcc ctgactcacc ttcttcttgc cattctcatc attttcgtca cgttccgggt
      481 gaagatacct gaaccggttg aggagggtct tcttgtcaac tttggttttg acgagacagg
      541 tgaagggttg tttgaaccgg caccggagcc tgtatctcct ccccctcctc ctgaatctgc
      601 aggggaacag ggaggtgagg aagcgctgct gacgcaggac ttcgaggagg ctgtggaagt
      661 ggaaaagaaa gagcccagtc ccgaggagat caggaagcag gcggaggcgc gtgctgcaga
      721 gataagaagg cagttggagg ctgaggctga gcggaagcgc atagaacagg aggaggccga
      781 gaagcggaaa aaggaggagg agcagcggcg tattgaccag cagaatgcaa gggccagggg
      841 cgctttcagc aatgcaggca atgtgggcac ttcaggccag agccagggag tggcaggcgg
      901 gcagggtaac cagggagttc agtcaggtac ccctgatgca cccaattacg ggccgggagg
      961 tggccaggga accgagggca tatcttttga cctcggagga aggaaggcac agtcactcat
     1021 caaacctccc tatgaccttc agaaagaggg agttgttgtt gttgcgataa cagtagaccg
     1081 ttccggaagg gtcaccgatg ctacacccgg catcaaggga tcgacgactc tcgaggggga
     1141 tctcctcaag ctggcaaagg aagctgcgtt gaaaaccagg tttgaaagca gcaatgatgc
     1201 cccgattatt cagaaaggga caatcaccta cgttttccgt ctcaaataaa actcttttgt
     1261 ttcagaacag cccggccaca aggtcataga ggccggtaag ctttattgcg gaagccataa
     1321 tggctacaag cagtattaca cgcactaccc tgggacccca gctgactgcc accttcgagc
     1381 cgagccaggc tcctgcaccc tgtccgacag ccaggatgat gccgtagaga taataaacct
     1441 ggttgctgag gatgaataca accagtgcga tggcagtcat ggcggccaca atcagcacct
     1501 tatgggcgtt ggccctggtc aggcccatgc cggtcccgag caccagcgcc gccagcagga
     1561 aaaagcccac cccggcctgg ataaaaccgc catatatgcc gatgatgaaa aacaccacag
     1621 gtacccacca ccgccgcttc atggccggtg agtgagcatg gtcacgtacc cactgttcag
     1681 gcttgtaaag gattacgaag aacatgaaga caagcagtcc tcctatcagc agttccatca
     1741 tccgttcatt gatcttcact gcaatgagtg accccagcag ggctcctgca atggcaggca
     1801 ctgacatcca taggctttcg ccgacggtat agagtttctg ctttttaaag cttatgctgc
     1861 tgaccaggct ctggacgaag acgccgatac ggttggtgcc atttgcaata ttggcaggca
     1921 ggccgaggaa catcagtaaa ggcagggtaa tcagtgaacc gctgccggca agagtgttaa
     1981 caaagcctgc aaagaggccg gcggctatta cagccaggta catgtaccat tccatatttg
     2041 ccgggaacta tctttccagg acttccttaa ctgccatcag gaggttgtca atcaggtcag
     2101 aatcctttat gacataatca gctatcccct ttcgtgcaaa ctcaagcacc acctctccct
     2161 tctcctgggc ggagagcatg atgacgggaa cttccggagc cagcctggta atctcgtcat
     2221 aggcttccat tccattcatg gggatttcgc cctctttcag gaaatagtag tcaaggataa
     2281 tcaggtcagg aatttcaccg agtgcagcga tgcaatcctc ggcataggca aatgccctta
     2341 cagcataacc attgccttcc agcctggttg agaccaggct ggtgacgaca ggatcatcat
     2401 caaccacata aacaaggatg tcagtctggg ggctaacttt ttccatgatc acaaaaatac
     2461 taaaggaatt gctaatttcc tagtacgcaa aaagagggag gtctttcatc atttttacca
     2521 ccttctctcc caccgatgtt atcactgatt cattttcaat gttaaccagg acctcatcaa
     2581 ggagctgaac gatggttccc atgtcaccct ccttcagtcc tcgggttgtg acggcagggg
     2641 tacccaccct gatccctgaa gtaaggaatg gcgagcggga gtcgaacggc accatgttct
     2701 tattgacggt gatgtgtgct ctgaccaggg tattttcaac cttcttgcct gaaatttccg
     2761 ggaagcgtgt tctcaggtcg atcagcatcg aatggttgtc tgttccgcct gatatcacct
     2821 tgtaaccctg ggccatgaaa gcctcggcca tcactgcggc atttttcttc acctgctgct
     2881 ggtaggtccg gaactccggt ttaagtgctt ctccgaatga gacggctttg gctgcgataa
     2941 tatgctccag gggtccgccc tgtatcccgg ggaacacggc tgagttgagc attgccgaca
     3001 tcatcttgat ctcgcccttg ggggtggtca cgccccaggg gttggggaag tctgttccca
     3061 ggaggatgat tcctccgcgt ggtcccctga gggttttatg tgttgtggag gtgacgacat
     3121 gtgcataacg aagcgggtta tcgagcaatc ctgctgcaat caggccggca gggtgtgcca
     3181 tgtcaatcat gagcagagcc cctgctttgt cagcgatagc gcgcatcctt gcatagtccc
     3241 attccctgga gtatgcagaa gcgccgccta cgatcagttt tggtttttcg cggagggcaa
     3301 cctcctccat catatcatag tcaacacgac cggtctcctc cttcaccccg taggcaaccg
     3361 gattgtacag tatgcctgac gagttcaccg gagatccgtg cgagaggtga cctccgtgtg
     3421 acagattcaa tcccagaaag gtatcgcccg gtttgagtat ggtcaggaag acagccatgt
     3481 ttgcctgtgc ccctgagtgg ggctgtacat ttgcatattc tgcattgaag agtttcttca
     3541 gcctgtcaat tgcgagctgt tcaacctcgt caacaacctc gcatccgccg taatatcttg
     3601 ccccggggta accttccgcg tacttgttgg taagcacgga acccattgcc tcaagcacct
     3661 gcgggctgac gaagttttct gatgcgatga gttcaatacc ttcagtctgg cgtttgcgtt
     3721 cgcggtcgat cagttcaaat attgcgctgt ctctttccat gattagattc ttagttcggt
     3781 tgataattac ccgtttcctc aagccggtga acacgggatg tttgcgggca aatatacatt
     3841 taataatcaa atttactcat gacccccgga tgtccggtga agtacgatat ggatacgata
     3901 tgggcaagcc ggatcattcc tctgaaaact gtctgagcag ttcgcggtat gcggaaaaga
     3961 ttcttgactt ggagataaat cccacgtacc ggccctcgtc aagcacagga aggttccagg
     4021 ctccggtgcg ttcgaaggcg tcgaggactg catcacctct ctcactgaga tcaatatagg
     4081 aaggaggtat ggtcatcagt tccctcacgg tgattttatc atagagttcg ggtctgaaca
     4141 ttttgtcacg gacgtcatcg agcagcacaa cgccctccag gatattgaat tcatccacca
     4201 cagggaagag gttccggttt gattttgaga tttgctttac aagatctccc aggttgctgt
     4261 cgggtctcac cgtcagcagg tccttttcca cctccctgga ccagtcgagc agagtcataa
     4321 ccgccttatc cttgtcatgg gtgatcagct ctccggttat cgcaagctgt tcattgtaaa
     4381 ttgaatactt ctggaatgtt ctggcagtga taaaggctac agtggatgtg agtatcaggg
     4441 ggatgagcag ggcatagcct tccgttatct ccgctataag gaagatagcg gtgagggggg
     4501 catgaagaac ggccgagaat actcctgcca tggcggtaag ggtgaaactg ctttcgatca
     4561 gggccaggcc gaacaggtga ttcagcaggc tggagaggta gtagcccgcc actcctccgg
     4621 taaacaatac cggagcaacg atgccgccta ctccaccggc accgtttgtg gaggctgtag
     4681 ccaccacctt gacaagcagc agtccggcca tcagcaatga gaaggaccag aatccggcag
     4741 ccatattgcc gaagatctca tttccggggg caactccgct tccggcattg aggagcacca
     4801 acaccgtttc gtatccctca ccgtagagcg ccggatagaa atagatcagg gctccgagga
     4861 tcgacccgcc tgccaccatc cttatccatt tgttgccaat ccttttgtag agtttttcaa
     4921 ttccgatggt tgcccttgta aagtatattc cgaccagacc acagaagaca cccaggatca
     4981 ggtagtatgg cagctcgctc agcatgaatc cctgttttac ctggaaagaa aaaagcacat
     5041 tgtcgcccat gaagaagaat gccacggttg ctgcagtcac agatgaaatc agaaggggaa
     5101 ctattgatga catcgtcagg tcaagcatca gcacctcgag ggtgaacaat attcctccaa
     5161 agggagcctt gaagattccg gaaatggccc cggcaacgcc acatccgagc agcagggtca
     5221 catacttgta attcagtttg aagtggcgtg ctatgtttga gcctatcgaa gcgcctgtaa
     5281 gaacgatggg agcctctgct cccaccgagc cgccgaagcc gatggtgagc gagctggtca
     5341 ggattgacga ccagttgtta tggggtttta tgtagctttt tcgtttcgag atggcataga
     5401 gcaccctgct gatcccatgt gagatattat ctctgacgag gtatttgact gccagcattg
     5461 taagaaatat ccctgcaagg gggtaggcaa gtgagagaac tcctccggcg cctttcataa
     5521 caccctgggt gaacagaaca tgggtaaagt gaattgcatt cttcatcacc acggctgcca
     5581 gtgcacttac aagtccgacc aggaaactga gaatgtaaat cagggtctgc tggttggtaa
     5641 ggaaagattt tagctgcctt attttcctaa ttatcaggtt gttcaaggtt accggagaaa
     5701 aatgaaaaat attcagatta caaatatatt ataaaatgag gatcagatgt ttattttggt
     5761 aagtttgaca tattttgcat acatgatcgg atttgaaagg caaatacttg acaacgggct
     5821 gagggtgata gtgcaccggg atgagggcac ccccatggta acaaccaata ttctttatga
     5881 tgcaggctcg agggatgagg agcctgattc cacaggtttc gcccatctct ttgaacacct
     5941 gatgttcgag gggacgcaga acatacctct ttttgacgtg cctgtgatga tggccggagg
     6001 tgagaacaat gccttcacca acaatgacct caccaactac tatatcaccc ttcctgcgac
     6061 caaccttgag acggcgttat ggcttgagtc agacaggatg cggggcctcg acctggcaga
     6121 ggagaggctg gccaaccaga agagtgttgt caccgaagaa ttcaggcagc gctatcttaa
     6181 ccagccatac ggcgatctgc tgctgttgct ccggcccctg gcctacaggg ttcatcccta
     6241 ccggtggccg gccataggga tggatatcag tcatattgag aaagcagaca gcatctcggc
     6301 aaaaaagttt tatgaccgtt tttacacccc ctccaatgca gtactcagtg ttgcaggcaa
     6361 cgttgatccg gacagggtgt tcagtctagc tgaaaaatgg ttcggagaca ttccgtcagg
     6421 tgtaaagacc gaaagaaagc tccctgttga gccggaacag gcagaacggc gcgagctgac
     6481 ggtacacagg cctgttcatg cccctcacct gctaatggcc tttcacatgg gaagtaggga
     6541 gagccgcgac ttttacatcc ttgacctgct taccgatata ctggccggat gtgattctgc
     6601 acggttccct gccatactcg tcaggaaaaa ggagctcttc agcgaagccg acatttatct
     6661 ctcaggcgaa gctgatccgg gtcttgtcat attcagtggc aggatcagag acgggattga
     6721 tgtcagaaag gcagaggaag cagtcaggtc agagcttgac aggctggcct ctgagcgtgt
     6781 aattgctgcc gaacttgaca aggcacgcaa caggtacgag tcgcaccgtc tgatgcaata
     6841 cataagcgtt tccaacaagg cattcagcct tgcctatcat gaggtgctcg gtgatgcaga
     6901 cggcatcaac agggagcatg aacagtacat gagtgtgaca gcagaagaga tagcctcagc
     6961 tgccgggctt acattcaggc ccgggaacag cagcgtgatc cattacctgc ccgagaagat
     7021 atgagaaaca aagagataaa gggagccccg gagatcatca ctccgtcagc gggagatact
     7081 tcactgccgg aaccggtgat gcttggcaac ggagtaccgg tctatatggc aggaaatggc
     7141 accgtcgatc tgctgagaat tgagttcgtt atccctgccg gccaggtgtt ggaggaggtt
     7201 catctggcag catccgctac gagcgcaatg ctcaccgagg ggacattgca tcatgatgcg
     7261 gctacaatca atgatctcat tgacagcacc ggagccgcgc ttacacattc ttctgacaag
     7321 gacacagccg gactggtaac ggttaccctg acaagaaagc ttgatgatgt gatggccctg
     7381 gccagtgagg tgctcttcag tccctccttt cctgaaaatg aattcaggat gctgacggac
     7441 agaaggttac aggcttttca gaccagcagg cggcggactt cggttattgc aagggaagct
     7501 ttctatgaag cactgtgcgg cagcaggagc ccttacggac gcattacaac ggaagatgat
     7561 taccgtagac ttacgacagg tgacgtgagg gagtttcacc ggaagcacta caggccggtg
     7621 aacatgtacg tcacggtagc cgggagtgag ccatggaaag cgcttcccgt actggaaaaa
     7681 tacttctcag gcaaaggaac gggacaatgg cagaagcctc ctctcccctc tcccgtattg
     7741 gaaacttcgg agcccggaaa gattttccgt gaccttccca cctcggttca gagcacggta
     7801 cgggtgggtt ggaaggggat tacccgcaac catcctgact accaggcctt gcaggtggcc
     7861 acaatgatac tcggcgggta tttcggctcg cgcctgatgc gtacaatacg cgaggagaag
     7921 ggttatacgt acggcatcgg ggcagttgcc ggcgctttcc ataacatagg ctatattacc
     7981 atcatgacag atgtggcaaa tgcctacagg gatgagactc taagtgaaat caggaaagag
     8041 attgagaagc tccgcacaga aggggtaagc gaggaagaga tgaccctggt aagaaatcac
     8101 atgatgggag agctggcccg gatctttgac ggaccgtttt cggttgccga tgctatcagg
     8161 ggcatcatcg actatgagac aggagcagac tattatacac gtttagccga aactgtcaaa
     8221 acaattacgc cagccaggat aatggatatc ttcaataggt atttcaatcc cggtgaggcc
     8281 tgggagatca ttgccggaag ccgatgaaaa agcagaatac cttaagaaag ctcttcatac
     8341 cgctgctgtt ctgcggtttg tctgcctttc cactggcagg acaggatgct gaccggcccc
     8401 tgtcacccag gcttgacatg gtgacggttg atcctgcaac cgggtttgcc gttctgaggt
     8461 ggcttccttc cccttcaccg gatgtgggca gttatgttgt ctacatctat gccgcaggaa
     8521 gggctgatgc cattgatacc atttattctc cattcgtttt cgaatacacc cataccgctt
     8581 cagctgccag gtacaggagc gtatcatatg tagtggcagc cattgactca tcagacaatc
     8641 cgagtccgct cagcaacagc ctcagcacca tatggctctc tgccgctgaa gatccgtgca
     8701 ccgcaacagt agcagtctca tggacagcat atgacaacca gtttcagccg ggcagtggtt
     8761 acctgttgca cataacagac ggaacgggag gatcgtttcc tgacatagcc ctgcctgtat
     8821 cttccacgca gtacctgttc accgggtacg aagcggatac agaatactgt ttttatgtaa
     8881 cggcaagcga cggctacggg ccgctttctt cgtcaaacag ggcctgtgtc acaaccggca
     8941 tcgaggctgc accgggatgg gtaaggatag atgcaattgc agtgcaggat ggcggcatat
     9001 catttgctgc cggctatgac ccgtcaacgg tgatgagtga tttccggctt tttcgctatg
     9061 atcctgttgc agccgcatgg gataaggcag cctcttcgac gggagttgcc ggcagggtca
     9121 catttgcaat ggcacaggca gacaccactg ttgtcaggct gtacagggtt gcagccgtga
     9181 acagttgtgg tctggcttca acagtttcag ccccatcccg gaatattttg ctggaggcat
     9241 caatcagcgg aacccttgtc aacctccggt ggaacaggcc ctctgccgga tggagcgggt
     9301 cattcagtgt ctggcgtgat atcggagagg gtatgcgcga agttgcttct tccatgtctg
     9361 acacgctctg gtcagatgat tacacaggat tcgcttcaga tgtatcagca ggcgaagttg
     9421 tataccgggt gtcaactgcg gatctctcag cgccggcagg cgccgggccg cacctttcct
     9481 ctgctgccgt aattgcaacc agtgagaatg tcttcatgcc caatgccttc acccccggcg
     9541 gtacggatga gaacgccatt ttcagacctg aattttcatt tcacccggga gaatatgatt
     9601 tcaggatact ttcaagaaac ggtgcactgc tgttccgcac gggtgacccc ggcgaaggat
     9661 gggacggcag gcacaacggc aggatgatgc cgccgggggt atacctctgg aacctgaggc
     9721 tcaccacccc ttccggacaa acccttgaaa ggaacgggac tgtaactata ctgccataac
     9781 agctaaaaaa ataatgagcc gattttcgta tctttgtcac tcgtttgcag gtagagactg
     9841 atggttaaga ccaggaccaa aatattcacc gctgatgata tcagccgtat cgagacagaa
     9901 tacagcctgc tgactgacaa tctttacagg tgtgacaagc ccggtgacag ggaattgatc
     9961 gagaaggcat tcaggttcgc gaatgaagcg catcgtgaca tgtaccgcaa ttccggggaa
    10021 ccctacatca ttcacccgat caatgtagcc cggatagtca accaggagat agggctgggc
    10081 gccaagtcgg tttcagccgc actgttgcat gatgtggtgg aagataccgg cattcccctt
    10141 gaggagatat cgaagcattt cggtcccaag attgcatcac tgattgatgg tctgaccaag
    10201 atatcaggaa cctacaacaa ggagaccaat tcactgcagg ctgagaactt ccgcaagatg
    10261 ctgatgaccc tctctgatga cttcagggtc atactggtga agatagctga caggttgcac
    10321 aatatgcgta ctcttgactc gatgcccgag tacaagagga tgaagattgc cggagagact
    10381 gtttatctct atgcaccgct ggcgcaccgg ctggggctgt ttgccatcaa gaccgagctg
    10441 gaggacctga gcttcaagtt caggcatccg cgcatctatg atgagatagc acgcaaggta
    10501 aagatggatg agaaaaagaa ccttgctctc atcaacaggt tcagcctgcc tatcatagag
    10561 gcgctgaaca acaacaatat cctgtttgac atatctggcc ggttcaagtc gatctattcc
    10621 atatggaaga agatgcagtc gaagaatgtc ccgtttgagg aggtatatga ccttcttgca
    10681 gtaaggattg tcttcgagcc cggtccggag atgagtgaga gggcgcagtg ctggcagata
    10741 tactctctta taacagatat ttacactccc aagcctgaca ggctgcgtga ctggatcagc
    10801 aggcccaagt caaacggtta tgaggcgctt caccttactg tgatgggccc tgaggggcgt
    10861 tgggttgagg tgcagatccg cagccagagg atggatgata ttgctgagag ggggtttgct
    10921 gcgcactgga agtacaaggg agacatgtct cagactgaga atgagctcga taaatggctg
    10981 aagcatatcc gggagatgct tgccaacccg atgtctgatc cgctcacctt cctcgatgaa
    11041 ttcaagatga gtctattctc ctcagagata atggttttca ccccaaaggg actgctggtc
    11101 aacctgccca agggagcttc cgccctcgat ttcgcatatg aaatccatac tgacataggc
    11161 aacaaatcca tcggtgccaa ggtaaactac aagctctccc ctctctccac cacacttcag
    11221 agcggagacc aggttgagat tatcacatcc aacattgcca ggcctgaaaa ggagtggctt
    11281 gagtatgccc atacggcgaa ggccaggaat gcgataaagg atgcgcttcg tgatgaatcc
    11341 actgatcgca tacagaaggg gatggacatg cttgagcaga agctgagtga gctggggatt
    11401 cttcccaatt ctgacatcat caagaagctt cttgacaact atgagctgct taacaaggac
    11461 gagctttatt cgcgcatcgg gctggggctt atcaacctcg acgagttgcg caaagtgctg
    11521 aagaaggctc ctgaaggaag cctgcgcaag tactggaagc tggccatcgg cggagttacc
    11581 cgcaagaatc cggaaacaag ggaggcgcct gtcactgctg aaaaaataga taagagtcag
    11641 ccctacctgc tccgggaaaa tgtcgacagc gctgccagtt cttacgtcat tgccagttgc
    11701 tgtcagccca tcccgggcga tgaagtggtt ggttatcttg accattccaa ctccatcatc
    11761 gtccacaagc ccaaatgccc ggtggccgta aagcttatgt caagcgaggg caacaggatc
    11821 attcccgtca ggtggaccat ccacaagctt gcttcattcc tggcgaggat aagcatcaac
    11881 ggaattgaca ggatcggtct tgtcagcgag gtgaccacaa tcatctcgaa ggagcttaat
    11941 gtcaatatca ggaacataaa cgtttcagtg tatgacggga tatttgaagg caccatagac
    12001 ctgttcgttc atcacacccg tgaccttaac aacctgatca tgcagctttc caacataaag
    12061 ggaatcgcca gcgtgaagag gatagaggag ttcattgagt agtcaatcag gcgaaacaat
    12121 caggcaaaat gtcaaacgaa gaagccaggg cggttgtcag gcaggttttt acggagtacc
    12181 ttgcaaccca ttcgctgagg aaaacacccg agcggtatgc aattcttgac gagatatatt
    12241 ccagggaagg gcactttgat attgaatcgc tcttcaagtt catgaataac aagaattacc
    12301 gtgtttcgcg ggctacgctc tacaacacta ttgaccttct gctcgactgc aaactggtga
    12361 caaggcataa gttcggccat aaccaggccc agtatgaaaa gacatacaaa gccggtcatc
    12421 atgaccatct gattgacacc atctcgggga aggtgatcga gttcagcgat ccgaggatca
    12481 atgacataat caggagcgcc tgcgaggaaa attcgttcgt tgtgcatcat tatgcccttt
    12541 atgtgttcgg cgtacaggaa aagagaggcg gatgagtggc ccgtggcgcc attttttttc
    12601 gtaacttcgg acctgttttg tctaatcaga gaatttttgg tacaatgaat attgacattt
    12661 tgctggggct ccagtgggga gatgagggaa agggtaagat agttgacgtc ctgaccccgg
    12721 attatgatat tgttgcccgt ttccagggcg gtccgaatgc cggtcacacg attgagtttc
    12781 acggggtgaa gcacgtgttg catcttattc cctcggggat ctttcatgat gacaagatca
    12841 acatcatcgg caacggcctg gtgattgatc cggttgtttt caggcatgag gttgagagcc
    12901 tggggattga tatcggaaag gtgcgtgaaa ggcttctcat ctcgctgaag gcgcacctga
    12961 taatgcccac tcataagctt cttgatgctg cccatgaggc ttccctgggt gaagcaaaga
    13021 taggatcaac tctccggggc ataggcccgg cgtatactga caaggcgtcg cgaaacggcc
    13081 tgagggtcgg ggatctgttc aggaaggatt tcagggagtt ttatcattcg cgggtaaatg
    13141 atcaccttcg gacccttcag ggttacggct atacaagtga tctgaaggcg ctggaggatg
    13201 agtggtttga cgcggtggag taccttaagc agttcaggac ggtaaatgct gattatcata
    13261 tcgaccaggc cctcagggcc ggaaaacgcg tccttgccga gggagcccag ggtaccatgc
    13321 ttgatataga cttcggtaca tatccttatg tcacatcatc gaacactgtg gctgcaggag
    13381 catgcacggg actcggaatt gctcccggac ggacaggtga agtgttcggc atcttcaagg
    13441 cgtactgcac cagggtggga agcggcccct tccctaccga gctcgatgat gctgacggtc
    13501 agcagatgcg tgaccagggc aatgagtacg gtgccacaac gggaaggccc aggcgctgcg
    13561 gatggcttga cctgcctgca ctgaaatatg caattatggt caacggggtg acacggctgt
    13621 tcatgatgaa ggctgacgtg ctctcagggt tcagtgagat aaaggtatgc aactcatacg
    13681 ctgtggatgg aagggaacag gcggaacttc cctattgcct gtatgacatc agcggactca
    13741 actacaggac cttcagggga tggcattgtg acatcacagg ttgcaggagc tgggaggagc
    13801 tgccccagga gttaaaggat tatatcaggt acattgaaga tgagacgggt gttcccgtaa
    13861 ccattgtttc ggtgggacct gacaggtcag ccaccatcac caggtaattg tcactcctgc
    13921 ctgtaaaccc aggcctcgcc attgatcctg tcctgaacag taccgagtgc attccatgcc
    13981 tggtgcaggt ttgggaaggc ctgagcagat acgagttgcc atcttccgcc attcatcccg
    14041 accagctgtg cgtcatagcc gaagctttta atatgttcca gccatgcatc tgcatattcg
    14101 ggtgtaagga agctgcccac gatgacatgg taaccggtca tcaccctggt ttgttcttca
    14161 gctgccacgg ccatctctgc ctgacgggcg cgttcggcct ccgcctgctt ctcgttggcc
    14221 acacggattg agtctgctat gcggaatgct tcctgctgct gacggagagc ctctgcctcc
    14281 ctggcctttt taccgaaggg atttatgctc ttcatgaaat cacatgaagg ggtaagaacc
    14341 aaaacggctg cgagcaacac gagtgatagt ttcttcataa atggcagttt ttcacgattt
    14401 tgattgataa aggtatggat aaatggttaa aaatgcaacc aatcttccgg aaagatgcca
    14461 ttcccagaca attctttcct atattagatc gctattttta acctcaaact acttatcgag
    14521 atgaagtcac ttatccggtt ctttgaagaa aatgtggaaa agtatgcatc caacatttac
    14581 atgtgggaaa aacttcagga gaaatatgaa ggtaccacct accaggagac caggcgccag
    14641 gttcatgaat tcggagccgg gctgatgcaa ctggggatta agaagggtga ccgtgtcagc
    14701 ctcctcgcag acggccgtaa cagctgggtc gtaggcgaac ttgggatact ttatgccggc
    14761 ggcgtcaatg tacctctctc catcaagctg aatcctgaag agatcaaatt ccgtctcatc
    14821 cactccgggt ccaggatggt aatcacttca agcacgcagg ccccgaaaat acttgcagtg
    14881 gcagaaaaat gcccggattt gcagaaaatc atcttcatgg atccgcgcga ggagtacgct
    14941 gaaggacaga tccatttcaa cgaggtaagg aagatcgggc gtgagtggct tgaaaatcct
    15001 gccaacagga aaagttttga ggagtttacc tcttcaatta ctgcttctga ttttgcaaac
    15061 atatgttaca catcgggcac gactgccgat cccaagggta taattctcac ccacggcaac
    15121 tatgtgacca acgtctacca gtcatacagt ctgatggaca ttccgcagtt ttacaaaact
    15181 ctcctcatac ttccgtggga ccactcattt gcacatactg cgggcatcta tgccttcatg
    15241 ggcaagggag ccagcatagc gtcggtaaag gtgggcaaga cgcccatgga aaccctgcgc
    15301 aacattcccg tctgcatgaa ggagataaag cccaacctgc tgatgagcgt gccggcgctg
    15361 gcgaaaaatt tcaggaagaa catcgaaaag gggataaagg aaaagggaag gatgaccgaa
    15421 gcgctgttta actttgcgat taagatggcc tattcctata acaaggaggg ctggaacaag
    15481 gggcgcggaa tacagaagat caagaaacct ctcctccgcc tctttgataa gatacttttc
    15541 agcaaagtaa gggaggcttt cggaggcgag ctcgaattct tcatcggcgg cggcgctctg
    15601 cttgacatcg agctgcagcg cttcttctat gccatagggg tgccaatgta ccagggatac
    15661 ggactctccg aggcatcacc catcatctca tcaaactcca ccgccaggca taaactggga
    15721 tcatcaggct acctggtcaa caacatggaa ctgaagatat gtgatgacaa cggcaacgag
    15781 ctgcctgttg ggcaaaaggg agagatagtc atccggggag gaaacgtgat gcacggatac
    15841 tggaagaacg atactgcgac aagtgatgcc ctgcgtgatg gctggctcta tacaggcgac
    15901 atgggataca tgagtgaaga tgggttcctt tatgtgctgg gccgcttcaa gagcctcctg
    15961 atcgctgatg acggcgaaaa attcagtccg gaaggaatag aagaggccat cagcgaacaa
    16021 tcgaaataca ttgaccagtg catgctctac aataaccaga agccgtacac tgtggccctg
    16081 atagtgccca accagcatgc catgaaactg taccttgaag accgcaacat gtcggccaca
    16141 accgaggaag gaaagcgtgc cgtcctcaat ctcatagaga acgaagtgaa tgaataccgc
    16201 ataaacggca agtacggtca catgttcccg caccgctggc tgccggtagc gatgggagtc
    16261 ctcaatgaga gcttcaatga agagaacggc atgatgaact caaccatgaa gattgtcagg
    16321 ggcaagatca ccgaccgata ccatgacctt atcgagtggc tctacacccc gatgggcaag
    16381 atagtataca atgaacgcaa catggaagaa gttgagaaga tgaaactcgg ctgaccagtt
    16441 aaaaccattc acagatgaaa tacttattaa ttcctgttct cgtcgtaatt gccgggatga
    16501 tgacctcttg caacagggag aaaaccccgg aatggaaacc tgctgaaaac ccgattttta
    16561 cagtctgggg tgagaaaatg gatccttcgg caccctggcc ggaatacccg aggccggcgc
    16621 ttgccaggga tgaatggatg aacctgaacg gtttatggga ttatgccatc tgtccgaaag
    16681 attcagatcg tccggaaccg cagggaaaaa ttctcgtccc cttccctgtt gaatcagctc
    16741 tctccggggt cagggaaagg atgagtgact cactggcact gtggtattca agggaattcg
    16801 ttctgccgaa acaatggaag gataagcgga tcattatcaa ttttgaagct tccgactggg
    16861 agactaccgt ctgggtcaat gacaggatgg cttcaatgca ccggggtggt tatgacccct
    16921 tctggtgtga catcaccaat tatattgatg acagaaagaa gcatacactt gtggttaagg
    16981 taacggatcc caccgacagg ggacctcagc caaggggcaa gcaggtaacg aagccgggtg
    17041 gcatctggta cactccaacg agcggcatct ggcagaccgt atggcttgaa ccggttgaga
    17101 agacctggat aggagatctt cgtgttgtgc ctgacattga caacggggtg cttcacgtga
    17161 cggtcatcga ggagcattgc cagggaggcc cgggggggtg ccctccggta gacatggcgc
    17221 cgtcacttgc ggagataacc ctgctggctg aaggcaaaac agtggctgtg gcatcaggga
    17281 taacaggatc cgtggtgact ctgccagtgc ctgatgcaag gctctggagc ccggatgacc
    17341 catacctcta tgacctgaag gtgaggctgg taaccggagg cctggttgct gaccaggtga
    17401 cctcctacgc tggcatgaga aaaatatcac tcggcgtagg tgaggatggc tttacccgca
    17461 ttttcctgaa caatgaattc atgtggcaga acggtccgct ggaccaggga ttctggcccg
    17521 acggactcta caccccacca tccgatgaag caatggttta tgaccttgag atgctgaaaa
    17581 agatgggatt caacatgctg cgcaagcatg taaaggttga gaacaggagg ttttattacc
    17641 acactgaccg gatgggcctc cttgtgtggc aggacatgcc cagcggcgac gactacatct
    17701 ggggtgacaa gcctgacatc gaaaagagtg catctgactc ggctgtcttc atggatgagc
    17761 tcacaatgat gatcggcacg aagttcaatc acccctcaat tgttatgtgg gttgtttaca
    17821 acgaaggctg gggccagtat aacacaccgg cagtaaccga ctatgtcatg gcccttgaca
    17881 ccacaaggct ggtcaacagc gcttcgggat ggaccgacag ggggacgggg catgtgcttg
    17941 atatacatca ctatcctgat ccagtggctc ccggccctga aaaagaccgg gccattgtgc
    18001 tgggcgagtt cggagggctg ggcctgccgg ttcagggaca tacctgggaa cagaagaact
    18061 ggggttatga gaagatgggt gacaccacgg cactcctgaa taagtatact gagttctatg
    18121 ctgcagtgcg acagatggtt gccgaaaaag gactgagtgc atcggtctac acccagacaa
    18181 ctgacgtgga gacggagacc aacggtctga tgacctatga ccg
//
LOCUS       DAOPXU010000056        18008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_3269_length_18008_cov_11.072077, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000056 DAOPXU010000000
VERSION     DAOPXU010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18008
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3269_length_18008_cov_11.072077"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(312..386)
                     /locus_tag="PKK03_07170"
     tRNA            complement(312..386)
                     /locus_tag="PKK03_07170"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(349..351),aa:Pro,seq:ggg)
     gene            543..1748
                     /locus_tag="PKK03_07175"
     CDS             543..1748
                     /locus_tag="PKK03_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNX84220.1"
                     /translation="MNQVNRRNFLRLAGAGAAAAAAAPVAQAMGAATAPVANVMNPET
                     AGRQTQQGKPDTNIADAAKIARGPDSMPGLYPGRVALVKNTRSVKDNLIVEQEVYDMI
                     ARSMLELTGEKKLKKAWRKFVKPGERIGLKVNPVAGKSLSTSHEVVRAVIAQLEESGI
                     KRSQLTIWDRREFELTDVGFTQENYPGIRIVGTEQMDKEGLYYGKDGKLYGEHMIDRD
                     WYYWADVEGEYDEYTMPYMVNGGKYSYFTKIVTQELDKIINIPILKNAGMTVTLALKN
                     LAFGSVSNTGRLHAKLWNETCAQVCAFAPLRDKVVLNIVDGIKGCFNGGPGANPQFFC
                     DYHSIIAGSDPVAVDRIGFDVVIDKRIAEGLQKSATPQGLEFMLQAEKLSLGVADREK
                     IELREVILG"
     gene            1773..2567
                     /locus_tag="PKK03_07180"
     CDS             1773..2567
                     /locus_tag="PKK03_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84221.1"
                     /translation="MLSGKFFAVASAALITVAAGAQSVVVPRALAGMNGVESAQSRTF
                     TETSLYGYINGGAELYLEYGFDTLLVTELVIEGSDIKAEVYRMKDPEAAFGIFSVSRF
                     RCNGGAKLTEHLCRSAYQLQFCKGPYYVSIINDTGSDDDQKRSSDLARLMIENIVEPS
                     FDPGSFYNDALDDDTMRGAVLVRGPLGIYNGIPGLSEKLGRASGYSALLIRHGTDTIA
                     SMKFNSDLAAEQFLREEKVRTGSGKVAVSSDSTVSVVSPRHIIITF"
     gene            2621..3625
                     /gene="trxB"
                     /locus_tag="PKK03_07185"
     CDS             2621..3625
                     /gene="trxB"
                     /locus_tag="PKK03_07185"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNX84222.1"
                     /translation="MQFFKPLETGSEEEKKPATATEKVRCLIIGSGPAGYTAAIYAAR
                     ANLKPVLYTGLQQGGQLTTTTEVENYPGYPDGVTGPEMMEDLRKQSERFGTEIRFGIA
                     TSSDFSGKPLKVTFDGSVEIEADTVIIATGATAKYLGIPAETKYAGMGVSACATCDGF
                     FYRGKDVAVVGGGDTACEEATYLAGLCRKVYMIVRRDVLRASKAMQHRVMNTPNIEIL
                     WKCQIEDLFGTQGVEGAVVVRGRGTPEEEKFNINIDGFFLAIGHTPNSDVFKPWMETD
                     ETGYIITDPGSTRTRVPGVFAAGDVADRVYRQAVTAAGSGCKAAIDAERYLAEQGQGI
                     "
     gene            3656..4042
                     /locus_tag="PKK03_07190"
     CDS             3656..4042
                     /locus_tag="PKK03_07190"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNX84223.1"
                     /translation="MRNFKKTFHINAEPSDIYAALTNPYTIELWSGYPAIMPSKAGEE
                     FNMWDGDICGRIIDLVPDRKVVQEWYFGDREVESQVTITINGNGGGTAVTVEHTNIPV
                     EDYQNIAEGWNEYYMGAIRRFYNPNF"
     gene            complement(4024..4692)
                     /locus_tag="PKK03_07195"
     CDS             complement(4024..4692)
                     /locus_tag="PKK03_07195"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HNX84224.1"
                     /translation="MTGNIDSLNTPEELLQAYTSLFLFLGGSVSMAPGESGLAHLCDT
                     GMLRNLPVSSNNSRFARATRLLKSPCPMKQQCHLAVGENYRSLLAGDSTAEAYPAASH
                     WFNRGRPAEEHHGELCSFYHRYGYSRADECGLTPDHLGIQLLFSNLLIEKYLTEDDNE
                     IRAMIGRDILRFISDEMLNWIPRWAEAVTAKSRTKCYTGISDLITGGLEDVVDILKSQ
                     KLGL"
     gene            complement(4742..5254)
                     /locus_tag="PKK03_07200"
     CDS             complement(4742..5254)
                     /locus_tag="PKK03_07200"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HNX84225.1"
                     /translation="MKKQLILVLILSALAIPMQAQTSPVDDLFEKYDGKEGFTSVYIS
                     SKMFSLLSKIDSNDEEFRNLVTRINGIRILSIDSADNTTRLNFATELMPKLRRNGFEE
                     LMTVKEEDDNVFFMIREVGDRIAELVMVTGGHGSTVVSIRGNLDLKTIASLSKSVGID
                     ELEGLENVKR"
     gene            complement(5266..5853)
                     /locus_tag="PKK03_07205"
     CDS             complement(5266..5853)
                     /locus_tag="PKK03_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84226.1"
                     /translation="MKWSEYTAMEQEKIRELLDKYFGGETSEDEERILREYLGSASAP
                     DSMTREFGYLAMKPEQVPEPSEGFETRLRDITRPEITMHHPDRRRILITIITTAAAVA
                     AGAWLLMSSLSLHGERDTYSDPVIALAEVRSILLDVSERMNTGTAQLEQVSTITAKPE
                     ELKVLSTINDLVGKNLSRLRYLDDLQNQGNKTETK"
     gene            complement(5795..6331)
                     /locus_tag="PKK03_07210"
     CDS             complement(5795..6331)
                     /locus_tag="PKK03_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNX84227.1"
                     /translation="MTPEEFRKRVLPEGRRLYALAFRYLNNREEAEDAVQEVMMKLWS
                     ERNEKRELSSVAAWCTTVTRNCCIDLLRRRKSLRIGDETVAEGIAAFVADPGKSHDNS
                     EAAHLIRVIISRMKEPGRSAVIMRDVEGYSYEETAEAMGMNVTALRTLMSRARKQVRD
                     EMVRIYGYGTGKDQRTAG"
     gene            complement(6359..6868)
                     /locus_tag="PKK03_07215"
     CDS             complement(6359..6868)
                     /locus_tag="PKK03_07215"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HNX84228.1"
                     /translation="MKRMVFAIAALAFAASLSAQRLSADEFFNKYSGRDGYTTVEVSG
                     SLLGILGCREDETADNPLRKITSIRVLVREKDKFPSAEGFIPEIRSIIKRGRYEELMT
                     VRDSDTDLRFMVRSESDMVREILLVVDGDDEAVIQIEGNLTRDEASRLFQSDGKGLSI
                     LEGLEISGD"
     gene            7092..9038
                     /locus_tag="PKK03_07220"
     CDS             7092..9038
                     /locus_tag="PKK03_07220"
                     /inference="COORDINATES: protein motif:HMM:NF027585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HNX84229.1"
                     /translation="MNASEAISLLEDKGVVLPEEVCLIARNSPKALFFNSSAEIFDAS
                     TPGRDDLTYEVRYNIPGKGEVVEVILHRVTNGLSANYTEPYMRRRDPETMLIGDSRPT
                     DKKRFQDAYGYPFARMKDETLEWLKGQEVGMFLCFAGNYPVGAGGVVVTPLNASFFAF
                     GLSLLQRIIAVDQLPDGYRVESAAYIAPPFRHTHFNGRQVVVHNRLEGLHEMFAYNLY
                     PGPSAKKGFYSVLLDKGEDEGWLTAHCSTVQVVSPYDNITTFMHEGASGGGKSEMIQN
                     LVREPNGQILIGQNIISGERRVISIPLFCSFHPATDDMALCHPSYQKNNGKLTVADAE
                     NAWFIRVDGVTGYGDDPFLEKITINPDEPLLFLNIDTRPDGTALIWNHTEDSPGKRCP
                     NPRVVLPRRIVPGVINRPVTVDVRNFGVRMPPCTADKPSYGIAGLFHILPPALAWLWR
                     LVSPRGYANPSITGTGDLESEGAGSYWPFATGRKVRHANLLLEQIVNTPRVRYTLTPN
                     QHIGAWKVGFKPQLLMREYLTRRGNAKLRADQLSPARCPLLGYELNYLTIEGSPVPSR
                     FLKVYRQAEVGTDGYDAGAEILKEFFRRELTGFLTPDLIPLGRRIINAFLEGCTVEDY
                     ARILPMEYQYSFLTINDYDSQGGQ"
     gene            complement(9056..10303)
                     /locus_tag="PKK03_07225"
     CDS             complement(9056..10303)
                     /locus_tag="PKK03_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNX84230.1"
                     /translation="MDIFDKIRKNRGALGQYQEQGDGYFMFPKLEGEIGPRMKFMGKE
                     VLNWSLNNYLGLANHPEIRKVDAEAAAQYGLAYPMGARMMSGQTTMHEKFEAMAAEFV
                     GKESAYLLNFGYQGMVSTIDCLAGRHDVIVYDSESHACIMDGLRMVLSKRFVYAHNDM
                     ESFEKQLQHAQNIVAETGGGILVITEGVFGMAGDLGALDKIVTYKKKYNFRLLVDDAH
                     GIGTMGRTGAGTGEHFGVQDQIDVYFGTFAKSFAGIGGFVAGDKEVISYLRFNLRSQI
                     YAKSLPMAMTIGAIKRLEMMKNHPELRERLWEIANALQKGFREAGFDLGRTQSPVTPV
                     FFNGELAQTTHLIKDLRKNYGIFCSVVIYPVVPRGVIMLRIIPTAAHTMEDVNYTLQA
                     FKEVKKKLETNVYPTDVMADFRN"
     gene            10494..11312
                     /gene="bamD"
                     /locus_tag="PKK03_07230"
     CDS             10494..11312
                     /gene="bamD"
                     /locus_tag="PKK03_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNX84231.1"
                     /translation="MRIKPITFLMLALIITSCGEYEKLLKSTDNELKRQKVFDYYDDG
                     KYTKSIELLSQILPRYRATDQAEQLSWINAQAHFRIRDYMMAGQYYQEFADNYPGSTN
                     AEEASYLAAMCDYYQSPRPELDQTNTRKAIESFTIFMQRYPTSTRNDDCKARILELKE
                     KLVEKSYLSARLYYDLKEYRAATVSLSNSLKEYPDTKYREELMFLKLDALYLLAVNSV
                     PEKKVERFQTTLDEYYSFIEEYPQSKFSKDVNRIFESTAKYLKVDTTVKQTENN"
     gene            11329..11664
                     /locus_tag="PKK03_07235"
     CDS             11329..11664
                     /locus_tag="PKK03_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNX84232.1"
                     /translation="MDYKKTSASYTTITRDIDKFDTTTGNIYESVIICSRRANQISVE
                     MKQELNSKLEEFASFNDNLEEVFENREQIEISKFYERLPKPTLISLAEFEDNKIYHRN
                     PDKEQKQKK"
     gene            11674..12885
                     /gene="coaBC"
                     /locus_tag="PKK03_07240"
     CDS             11674..12885
                     /gene="coaBC"
                     /locus_tag="PKK03_07240"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNX84233.1"
                     /translation="MLKGRKILLGVTGGIAAYKAAVIIRLLIKEGAEVKVVMTPLAKE
                     FITPLTLATLSKNPVMADFFDPEDGRWNSHIDLGLWADLYLIAPATANSMGKMAAGIA
                     DNLLLTTYLSARCPVFLAPAMDLDMLAHPATIGNMERLKSYGNHIIEPDTGELASGLE
                     GKGRMAEPEVIVRELREFLSKKKITEQPLKGRKVLINAGPTVEPIDPVRYISNYSSGR
                     MGIALAEAAAVMGGDVTLVLGPVSIKPRSGDISIVPVTTAAEMKEATVAAFSTCDIAI
                     LAAAVADFTPEHPHETKVKRGDDDMVIRLKPTEDIAACLGKMKRKGQLLAGFALETDN
                     ELESAKGKLRRKNLDLIILNSLKDQGAGFGHDTNRITIIDSNDNIDKFELKTKDEVAS
                     DILKKIISLTR"
     gene            12892..13824
                     /locus_tag="PKK03_07245"
     CDS             12892..13824
                     /locus_tag="PKK03_07245"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HNX84234.1"
                     /translation="MKQPVKTRLLVAVLLMALTTGPAVSQELYCNVQVSAQKIQGSNR
                     EVFQNMQRDIYEFMNSMVWTDNIFSFSERIECNLLINLDEQLSADEFRGTIQVQLSRP
                     VYNTTYESTVLNFVDNNFQFRYVEFQPLEFNPNSHTSNLVSVLAYYAYLLIGMDFDTY
                     SQGGGTPYFQVAEKIVTNAQNAPEQGWKPYDGSRNRNRYWLVKNILDSEYSGVRQFIY
                     LYHLRGLDRMESDMTQARTEIYESLRRLQEVYRRRPDPFMYYMTVVLDAKADEIVNIF
                     TQGFPEEKNRVVQVLTEIDPANETKYQKILTSNS"
     gene            13835..15493
                     /gene="recN"
                     /locus_tag="PKK03_07250"
     CDS             13835..15493
                     /gene="recN"
                     /locus_tag="PKK03_07250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNX84235.1"
                     /translation="MLRRLTVRNYAIIKDLNMEFGEGFTIITGETGAGKSILLGALGL
                     VLGERADSSVLLSQDDKCIVEACFNIGGYDLNELFEANEIDYDEEAIFRREITPAGKS
                     RAFLNDTPVNLTVLKELGSRLIDVHSQHETLLLGAGFFQMRVVDAYAGISNLSGDYAR
                     AWKGYIAARRDYDTAMAGLEKVKADFEYYSHQLEEFRAVKPVTGEQEVLEKEQEMLSN
                     ASEIKEALAAVTAALGGEEISALSLLRTVRTNLTRISGWFPGAAELEKRVEAQLIDLN
                     DISYEAERLNDRASADPGRLEYVTQRLDALYSLMQKHRSRDLDELLAVQNRIEEAVAA
                     SSDTDEKTEELRKRRDTALAQVNSLAGKVSEGRIKAVAPLSHEITGMLHRLGMPHARF
                     EASVKPLPAPGPSGMDEIGFLFSANRQVEPEELSKCASGGELSRVMLCLKSVLANSSG
                     LPAIIFDEIDSGVSGEVASMVGSILADMGRSMQVINITHLPQVAARGRIHYHVYKEEG
                     DHSTITRVRLLNDDERVREVARLLSGSTVTDAAMRNARELMKDN"
     gene            15530..16366
                     /locus_tag="PKK03_07255"
     CDS             15530..16366
                     /locus_tag="PKK03_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX84236.1"
                     /translation="MIKNLLEGKKGIVFGALNDKSIAWHVAEKAHAQGARLVLTNAPV
                     AIRMGTIGELAEKTGSEVISADATSMKDLEVLFTRSMEIFDGKIDFVLHSIGMSPNVR
                     KGIPYAESSYENMLKTLDISALSFHRILQTAYKMDAIREWGSVIALTYVAAQRTLAGY
                     NDMGDAKALLESIARSFGYIYGKEKHVRVNTVSQSPTATTAGAGIAGFDSLVDFSDRM
                     SPLGNADASDCADVCITLFSDLTRKITMQNIFNDGGFSSMGMSERAIEQYNKSFRECP
                     DK"
     gene            16466..17749
                     /locus_tag="PKK03_07260"
     CDS             16466..17749
                     /locus_tag="PKK03_07260"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HNX84237.1"
                     /translation="MLLALLLIVFYFTFPLVFIWMCKRWSLLQKLGAIVLAYGFGLII
                     GTAGFLPKGSEGYREALQGEATMSTGTLDLLIAEGKAVPEDHTANRIAGIQDKVYTVS
                     LLVAFPLLLFSLNLKRWLKYAKKGFVSIGLALVAGLVMVTAGFFIWKNSFPDTWKLAG
                     MFEGIYTGGTPNFAALKLILDVDAERFVVLNTYDMIVGAFLVLFFVTIAPAIFRAFLP
                     KFREEKGVALDEELVKQETEGLDDFSGMLDKQRIWPLIRALLMSILIAGVGAVVYFLI
                     PPDLKQYRMAGMVLTITTLGILASLYRPINQIKKTFQLGMYLILVFSLTVASMSDLRT
                     MFTKGMLDLMLFISWCYFGSLFLHIILAKIFRIDADNFLITATAFIFSPPFVPLVANA
                     LKNKDVIVTGIAGGVIGYTLGNYFGTTLAFFLQQF"
BASE COUNT         4540 a         4358 c         4743 g         4367 t
ORIGIN      
        1 caccgaaatg ttcctggaac tgcgtaatca gattatcacc aggtacacca ataaccggga
       61 gtaaacattt gtttcagttt taagtccggg aacggtaagg atcatactca agcacatgcc
      121 atagaggccg ggattgcgct ctccgccagc cggcggatcg cgcgatccct tctgggttat
      181 caaattgagc aaaaagctct gtcatgctgc gcatgacctg gccttttttg cctccagatc
      241 ttccggcagc atactgccgt catctgtcag caaaaaagcc ccggcacgtc gtgccagagc
      301 tttttgctca gtcgggatgc cgggatttga acccggggcc ccttgccccc cagacaagta
      361 ctctaaccgg gctgagctac atcccgtgct ttttaagcgg tgcaaaatta attaactatt
      421 ctgattatgc aaaaaaaagc tgaagcctgc tctccggcat aaaaattgaa atatgactca
      481 ccggcgtaat tatttatatt tgtttgtgct ttaaatacgt tatcatctca cagaaaagaa
      541 tcatgaacca ggtcaatcgc cgcaatttcc tccgcctggc gggcgcagga gcagccgccg
      601 ctgcagcagc accggtggca caggcaatgg gagcggcaac agcccccgtt gccaatgtca
      661 tgaacccgga aactgcaggc cggcagactc agcagggcaa acccgacacc aacatagctg
      721 atgcggcaaa gattgcccgg ggcccggatt caatgcctgg cttgtatccc ggacgggtgg
      781 ccctggtgaa aaacacccgt tcagtgaagg ataacctcat tgtggagcag gaggtatatg
      841 acatgatagc ccgctccatg ctcgaactga caggagagaa aaagctgaaa aaagcctgga
      901 ggaagtttgt taaacccggc gaaagaatcg gccttaaggt aaacccggtg gcaggaaaga
      961 gcctcagtac cagccatgag gtggtgaggg cggtgatagc ccagcttgag gagagcggta
     1021 taaaaagatc tcagcttaca atatgggacc gtcgtgagtt tgaactgaca gacgtgggat
     1081 tcacacagga aaactacccc ggaatccgga tcgtcggcac cgaacagatg gacaaggagg
     1141 gtctttacta cggcaaggac ggcaaacttt acggcgagca tatgattgac cgcgactggt
     1201 actactgggc tgacgttgag ggagaatatg acgagtatac catgccctac atggtcaacg
     1261 gaggcaagta ttcatacttt accaaaattg ttacgcagga gcttgacaag atcatcaata
     1321 tccccatact taaaaatgcc ggcatgactg tgacgctggc gcttaaaaac cttgccttcg
     1381 ggtcggtctc caacacaggg aggcttcatg cgaagctgtg gaacgagaca tgcgcccagg
     1441 tatgtgcctt cgcgcctctg cgcgataagg ttgtcctgaa catcgttgac ggcatcaagg
     1501 gatgcttcaa cggtggtccc ggcgccaacc cgcagttctt ctgcgactat cactccatta
     1561 tcgccggatc cgatcctgtt gcggttgacc gcatcgggtt tgatgttgtc atcgacaaaa
     1621 gaattgctga agggcttcag aaatcagcca ctcctcaggg actggagttc atgctgcagg
     1681 ctgaaaagct gtcgctgggt gttgccgaca gggagaagat cgagctgagg gaggtgatac
     1741 tgggataaaa cttttggata tttatatttg aaatgctttc agggaaattt tttgcagttg
     1801 catcagcagc gcttatcacg gtcgctgccg gtgcacagtc tgttgtagtg ccccgcgccc
     1861 tggccgggat gaatggagtt gagtcagccc aaagccggac atttacagag acgtcccttt
     1921 atggttacat aaacggtggt gcagagttgt acctggaata cggttttgat acgctgcttg
     1981 tcactgagct cgtgatcgaa ggcagtgaca tcaaggctga agtgtaccgg atgaaggacc
     2041 ctgaagctgc ttttggcatt ttctccgtct cgcggttccg ttgcaacgga ggtgcgaagc
     2101 tgacggagca cctgtgccgt tcggcttacc agcttcaatt ctgcaaggga ccatattacg
     2161 tcagcataat caatgacacc ggcagcgacg atgatcagaa acgctcctct gacctggcca
     2221 ggctgatgat tgaaaatatt gttgaaccgt catttgaccc gggcagtttc tataatgatg
     2281 ccctcgatga tgataccatg cggggtgcgg tactggtcag aggtccgctc gggatctata
     2341 acggcattcc gggattgtct gaaaaacttg gcagggcttc cgggtacagt gccctgctga
     2401 taaggcatgg cacggataca atcgcatcca tgaagttcaa cagtgacctt gctgccgagc
     2461 agttcctccg tgaggagaag gtcagaacag gttccggaaa ggtcgcagtt agcagtgact
     2521 caaccgtttc agtggtttcg ccccgacata ttattattac tttttgatat cggtttcatt
     2581 tatttgctat ttttgtcgtc ttaatgcaga aaatagcaag atgcaatttt tcaaaccgct
     2641 tgagacaggc agtgaggagg agaagaagcc tgccactgcg accgaaaaag taagatgtct
     2701 tatcataggc tcaggacctg caggatacac tgcagccata tatgctgccc gcgcaaacct
     2761 gaaaccggtg ctctacaccg gattgcagca gggcggtcag cttaccacta ccaccgaggt
     2821 tgagaactac ccggggtatc ctgacggtgt cacaggcccc gagatgatgg aagatctccg
     2881 caaacagtcc gaaagattcg gaacggagat acggtttgga atagcaactt catcagattt
     2941 ctcggggaag ccgctgaagg tcacctttga cggcagcgtg gagatagagg cagataccgt
     3001 gataattgca accggcgcca cggcgaagta tcttggcata ccggccgaaa cgaaatatgc
     3061 cggaatgggt gtttcagcct gcgccacgtg tgacggattc ttctaccgcg gaaaggatgt
     3121 ggcagtggtc ggaggcggtg acactgcatg cgaggaggca acataccttg caggcctctg
     3181 ccggaaggtt tacatgatag taaggcgtga cgtgctccgg gcaagcaagg ccatgcagca
     3241 cagggtgatg aacacaccga atatcgagat tctatggaaa tgccagattg aggacctttt
     3301 cggaacacag ggagtcgagg gtgctgtcgt cgtcagaggc aggggaacgc ctgaagagga
     3361 gaagttcaat ataaatattg acggattttt cctggccatc gggcatacac cgaactcgga
     3421 tgttttcaag ccatggatgg aaacagatga gacaggatac ataatcaccg atccgggatc
     3481 aacacgtaca cgcgttccgg gggtgttcgc cgcaggcgat gtggctgacc gggtgtacag
     3541 gcaggctgtc actgctgccg gaagcggctg caaggcggcc attgacgctg agagatatct
     3601 cgcggaacag ggacagggaa tttaaacaaa taaaaagggg aaacaaacgc tggacatgcg
     3661 aaattttaaa aaaacatttc atataaatgc cgaaccatct gacatttatg ctgcactgac
     3721 aaatccttac acaattgagc tctggtcggg ttacccggcc ataatgcctt cgaaggcagg
     3781 tgaggagttt aatatgtggg acggggatat ctgcggacgg attattgatc ttgtgccgga
     3841 caggaaggtt gtgcaggagt ggtactttgg cgaccgcgag gttgagtcac aggttacaat
     3901 caccataaat ggcaacggag gtggaaccgc agtaaccgtt gaacatacca atatcccggt
     3961 ggaagactac cagaatattg cagaaggatg gaatgagtat tacatgggtg ctatcaggcg
     4021 attctacaat cccaacttct gactcttaag aatatcaacc acatcctcca gtccgccggt
     4081 gatcagatct gatatcccgg tgtagcattt cgtccttgac tttgcggtga ctgcctcggc
     4141 ccaccgggga atccagttga gcatttcatc gctgatgaac cggaggatat cccttcctat
     4201 cattgccctt atctcattgt catcttcggt aaggtatttc tcaatgagaa ggttggagaa
     4261 caggagttgt atgcccaggt gatcgggtgt caggccgcac tcgtcagccc tgctgtagcc
     4321 atacctgtga tagaagctgc acagctcccc gtggtgttct tccgcgggac gccctctgtt
     4381 gaaccagtgt gatgctgccg ggtacgcttc ggcagtgctg tctccggcaa gcagtgaacg
     4441 gtaattttca ccgacggcga ggtgacactg ttgcttcatc ggacagggtg attttagaag
     4501 ccgggtggcc cttgcgaatc tgctgttgtt gcttgatacg ggaagattgc gaagcatgcc
     4561 ggtatcacac aggtgtgcaa gaccgctttc gccgggtgcc attgagaccg atccgccgag
     4621 aaaaaggaaa agtgaagtat atgcctgaag gagctcttca ggagtattca agctgtctat
     4681 attgccagtc atcagataat caggttgcat accccaataa cgtgatgacc gtgcaaattg
     4741 ttcatctctt tacattctca agcccctcaa gctcatcaat gccaacgctt tttgacagac
     4801 tcgctatggt tttaaggtca agattgccac ggatcgacac aacagtactg ccatggccac
     4861 cggtaaccat taccagctcg gctatcctgt cgccaacctc gcggatcatg aagaagacat
     4921 tgtcatcctc ctcctttacg gtcatgagct cttcaaaccc gttgcgcctg agctttggca
     4981 tcagctcggt ggcaaagttc agccttgtag tgttgtccgc actgtcaatg ctcaggattc
     5041 ttattccgtt gatcctggtg acaagattgc ggaactcctc atcgttgctg tcaatctttg
     5101 agaggagact gaacatcttc gacgagatgt ataccgaagt aaatccctct ttcccgtcat
     5161 acttctcaaa gaggtcatct accgggctgg tctgagcctg catcgggatg gccagtgctg
     5221 agagaatcag gacaagtatc aactgttttt tcatgatatc gtgtgttatt ttgtttccgt
     5281 tttgtttccc tggttctgaa gatcatcaag gtatctgagc cgggacaggt ttttgcctac
     5341 aaggtcgttt atggtggaga gtactttcag ttcctccggt tttgcagtga ttgtgcttac
     5401 ctgctccagc tgtgcggtac cggtattcat ccgttctgaa acgtcaagca atatgctcct
     5461 gacttcagcc agggctatga caggatcact gtaggtatcc ctttctccgt gaagtgacag
     5521 gctgctcatc agcagccatg ctcctgctgc cacggcagct gcggtggtga taatggttat
     5581 caatatccgt ctgcggtccg ggtgatgcat tgtgatctcc ggacgggtga tgtccctgag
     5641 cctggtttca aaaccttccg aaggttcagg tacctgttca ggtttcatgg cgaggtaccc
     5701 gaattcacgg gtcatggagt ctggcgccga agcgctgccc aggtattccc tgagaatgcg
     5761 ctcttcatcc tcgcttgtct ctcccccgaa gtatttatcc agcagttctc tgatcttttc
     5821 ctgttccata gccgtatatt ctgaccattt catcccttac ctgtttcctt gcccttgaca
     5881 tcagtgttcg gagggcagtt acgttcattc ccatagcttc agccgtttcc tcgtaggagt
     5941 atccctctac atccctcata atgacggcgc tcctgcccgg ttccttcatc ctgctgatga
     6001 tgacccggat caggtgtgca gcttcgctgt tatcgtggct cttcccggga tcggcaacga
     6061 aggctgcgat tccttcggcc acagtctcat cacctattct caggctcttt cttctcctga
     6121 gcaaatcgat gcagcagttg cgtgtcaccg tggtgcacca ggctgccacg cttgacagct
     6181 cccttttttc attcctctca gaccaaagct tcatcatcac ctcctggact gcatcctctg
     6241 cttcctcccg gttgttcagg taacggaagg cgagggcata aagccttcgc ccctccggca
     6301 atactctttt cctgaactct tcgggggtca ttatcctgga aactgttccc caaagttatc
     6361 aatcacctga gatttcgagc ccttcaagga tgctaagtcc tttcccgtcg ctctggaaga
     6421 gcctggaagc ctcatcgcgt gtcaggttgc cctctatctg tatcacggcc tcatcatcac
     6481 cgtcaacaac gaggaggatc tccctgacca tgtcactttc gcttcttacc atgaagcgga
     6541 gatcggtgtc actgtccctg accgtcatca gttcctcata acggcctctt ttgataatgc
     6601 ttctgatttc gggaataaag ccttccgcag aagggaattt atccttttcc ctgaccagta
     6661 ccctgattga ggtaattttt ctgagcggat tatcggcagt ttcatcttca cggcatccca
     6721 ggataccgag caggctgccg ctgacttcca ccgtggtgta tccatcccgg ccgctgtact
     6781 tgttaaagaa ctcatctgcc gagagtctct gtgcagatag tgaagcagca aaagcaagtg
     6841 ctgcaattgc aaaaaccatt cttttcatga ctgtccgttt ttcagttttt agtgttaact
     6901 gaatcaatga cgaaatggaa tcaaaaacat aacaggaatt cccgggtttt tgaaaaagtg
     6961 ttgatatctg tttatgactg ccgtaaggtt caggatgcaa tggtgttatt tttatcggcg
     7021 gaattatgaa taaatttacc gagacaagca gttggaatta aagaaattaa ccggaactta
     7081 atatttgtaa aatgaatgca tctgaagcga tttccctttt ggaggataag ggggtggtgt
     7141 taccggaaga ggtttgcctt attgcgcgaa attctcccaa ggcgttattc ttcaattctt
     7201 cagccgagat atttgacgca tctacccccg gaagagatga cctgacatat gaagtcagat
     7261 acaacattcc cgggaaaggg gaggtggttg aagtaatcct ccacagggtc acaaacgggc
     7321 tctctgcaaa ttacactgag ccgtacatga ggaggcgcga tccggagaca atgctgatag
     7381 gtgactcacg tccgacagac aagaagaggt ttcaggatgc atacgggtat ccgtttgccc
     7441 ggatgaagga tgagacactt gaatggctga aagggcagga ggtgggaatg ttcctctgtt
     7501 ttgccggaaa ctacccggtg ggagccggcg gtgtggttgt cactccgctt aatgcatctt
     7561 tttttgcttt tggactgtca ttgctgcaaa gaattattgc tgtggaccag ttgcctgacg
     7621 ggtacagggt ggagtcggct gcctatattg caccaccttt ccgtcatact catttcaacg
     7681 ggcgccaggt tgttgttcac aacaggctgg aggggctcca tgagatgttt gcatataacc
     7741 tgtacccggg tccgagtgcg aagaagggat tttacagcgt tctcctcgac aagggggagg
     7801 atgagggatg gcttacggca cactgttcga ctgtgcaggt tgtgagtccg tatgacaata
     7861 ttacaacctt catgcatgag ggggccagcg ggggaggcaa gagcgagatg attcagaacc
     7921 tggttcgtga gcccaacggc cagatcctga tcgggcaaaa tataatctcc ggggagagga
     7981 gggtcatctc aataccgttg ttttgctcgt ttcatccggc aacggatgac atggcccttt
     8041 gtcacccttc ttaccagaag aataacggca agctcaccgt tgctgacgcg gagaatgcct
     8101 ggttcatccg tgtcgacggt gtgaccggtt acggtgacga tcctttcctg gaaaagataa
     8161 caatcaaccc ggatgagcct ttactttttc ttaacatcga taccaggcct gacggcacgg
     8221 cgctcatctg gaatcatacg gaggactcgc ccggcaaacg gtgccccaat cccagggtgg
     8281 tgcttcccag aagaatcgtt ccaggggtaa taaacagacc tgtcaccgtt gatgtccgca
     8341 atttcggcgt gaggatgcca ccatgcactg cagataaacc cagctacggg atagcgggtc
     8401 tgtttcatat ccttcctccg gcgctggcat ggttatggag gctcgtcagc ccgcgtggct
     8461 atgccaatcc gagcatcacc ggcaccggcg accttgaaag tgaaggggca gggtcatact
     8521 ggccctttgc aaccgggagg aaggtaaggc atgccaatct tctcctggaa cagattgtca
     8581 acaccccgag ggtcaggtat accctgacgc cgaaccagca cataggggcg tggaaggtgg
     8641 gcttcaagcc tcagttgctc atgagggaat acctgacacg ccggggtaac gcgaaactga
     8701 gggctgatca gctgagcccg gcaaggtgcc cgcttctggg atatgaactt aattacctta
     8761 caatagaagg atcaccggtc ccctcaaggt ttctgaaggt ttaccgccag gcggaagtgg
     8821 gaacggatgg ttatgacgcc ggcgctgaaa tcctgaagga gttcttcaga agggaactga
     8881 caggatttct cactcctgat ctgattccgc tggggcgaag gatcataaat gcattccttg
     8941 agggatgcac ggtggaggat tatgcccgga tactgccgat ggaataccag tattcgttcc
     9001 ttacgataaa tgattatgac agccagggcg gccagtaaaa gtcctgatcc gtctgtcagt
     9061 tcctgaagtc tgccatcacg tcagtagggt aaacgttggt ctcaagcttt ttcttgactt
     9121 ccttgaaggc ctgtaaggta taattgacat cctccatcgt gtgagctgcg gtaggtatga
     9181 tcctgagcat gatgactccc ctgggcacta ccgggtagat tacaaccgag cagaagatgc
     9241 cgtagttctt gcgaagatcc ttgatgaggt gtgttgtctg ggccagttca ccattgaaga
     9301 agaccggggt tacgggtgat tgtgtgcggc cgaggtcaaa tccggcttcc cggaagccct
     9361 tctgaagtgc gttggcaatt tcccacagtc tctcgcgaag ctcgggatgg ttcttcatca
     9421 tctcgaggcg cttgatggct ccgatagtca tggccatcgg gagcgacttg gcatagatct
     9481 gcgaacgaag gttaaatctc aggtagctga tgacttcctt gtcgccggcg acgaatccgc
     9541 ctattcctgc gaagcttttc gcgaaggttc cgaaatagac atctatctgg tcctgaacgc
     9601 cgaagtgttc gccggtgccg gctcccgttc tgcccatggt gccgatgcca tgggcatcgt
     9661 cgacgaggag gcggaagttg tatttctttt tgtaggttac aattttgtca agtgctccga
     9721 ggtcgccggc catgccgaat acaccctcag tgataaccag gatgcctcct ccggtttcag
     9781 cgacgatgtt ctgtgcatgc tggagctgct tttcgaagct ttccatgtca ttgtgcgcgt
     9841 acacaaacct ttttgagagt accattctca atccgtccat gatgcaggca tgtgattcgg
     9901 agtcatatac tatgacgtca tgccttcctg ccaggcagtc tattgtggag accatcccct
     9961 ggtaaccgaa gttaagaagg tatgcggatt cctttccgac gaattcggca gccattgcct
    10021 caaatttttc gtgcatggtt gtctggcctg acatcatcct ggcacccatc gggtatgcca
    10081 gtccgtactg tgctgcagcc tcggcgtcaa ccttgcgtat ctcaggatgg ttggcgagtc
    10141 ccaggtagtt gttgagactc cagttaagca cctccttacc catgaatttc atgcgggggc
    10201 ctatctcccc ttcaagcttg gggaacataa aatatccgtc accctgttcc tggtactgtc
    10261 cgagcgcacc acggttcttt ctgatcttgt cgaaaatatc cacgtcagta tagttttgat
    10321 tattgcccgc aaaattatca aaaacattgt accgtcaaag tacctattta tacctatttg
    10381 ttctgtggaa ttaacactct cttaactact ctgtttcaat tattggtgca cttttgtggc
    10441 gtattgggtc aaccaataaa aaaaaagagt aattttgcgc aacatttccg ggtatgagga
    10501 tcaaaccgat aacatttcta atgctggctc tcatcatcac atcatgcggt gagtatgaga
    10561 agcttctcaa gagcacagat aacgagctca agagacagaa ggtatttgac tattatgatg
    10621 acggtaaata cacaaagtcc attgagcttc tctcacagat tctgcccaga tacagggcta
    10681 ccgatcaggc tgaacagctc agctggatca atgcccaggc acatttcagg attcgtgact
    10741 atatgatggc ggggcagtat taccaggagt ttgctgacaa ctacccgggc agcaccaacg
    10801 ccgaggaagc ttcatacctg gcagctatgt gcgattatta ccagagtccc aggccggaac
    10861 tcgatcagac aaacaccagg aaggcaatag agagctttac gatattcatg cagcgatacc
    10921 cgacaagcac ccgtaatgat gactgcaagg caaggatcct tgagcttaag gaaaagctgg
    10981 tcgagaaatc atacctgagt gccaggctgt actatgatct taaggagtac cgcgccgcaa
    11041 cagtatcact gtcaaacagc ctgaaggagt atcctgatac aaaataccgg gaggagctga
    11101 tgttcctcaa acttgatgcc ctctacctcc ttgccgtgaa cagcgtccct gagaagaagg
    11161 ttgagcgctt ccagacaaca cttgacgagt attattcgtt tatagaagag tatcctcaga
    11221 gcaaattctc gaaggatgtt aacaggatat ttgaaagcac ggcaaagtac ctgaaggttg
    11281 acacaacagt gaaacaaaca gaaaataact gaaatcatca acacaaatat ggattacaaa
    11341 aagacatctg catcctacac aaccattaca agggatattg acaagttcga caccacaacg
    11401 gggaatatct acgaaagcgt cattatctgc agcagaaggg caaaccagat ctcggttgag
    11461 atgaagcagg agctgaactc aaagctggaa gaatttgctt cgttcaatga caatctggaa
    11521 gaggtttttg agaacaggga acagatagag atatcaaagt tctacgagag gcttccaaag
    11581 cccacactga tttctcttgc cgagtttgag gacaacaaga tatatcaccg caatcctgac
    11641 aaggagcaga agcagaagaa ataactggcc aacatgctga aaggcaggaa aatactgctt
    11701 ggagtcaccg gaggtatagc agcctacaaa gctgctgtca tcatccggtt gcttattaag
    11761 gaaggggctg aggtaaaggt tgtgatgacc ccgctggcaa aggagttcat cacgccgctg
    11821 accctggcca ccctttcaaa aaatccggta atggcagatt tctttgatcc ggaagacgga
    11881 cgatggaaca gtcacatcga cctgggtctc tgggccgacc tttatcttat tgctccggca
    11941 actgccaaca gcatgggaaa gatggccgca ggcattgcag acaaccttct actgactaca
    12001 tatctctcgg cacggtgccc ggtcttcctt gccccggcaa tggacctgga tatgctggca
    12061 cacccggcaa caattggcaa tatggagagg ctgaagtcgt atggcaatca catcattgag
    12121 cctgataccg gtgagctggc cagcggactc gaaggcaagg gccgtatggc cgaaccggag
    12181 gtaatagtga gggagctcag ggagttcctc tcaaaaaaaa aaataactga acagccgctg
    12241 aagggccgga aggtcctcat caatgcagga cctaccgtgg aaccgataga tccggtccgg
    12301 tatatcagca attattcttc aggccgcatg gggattgcac tcgctgaggc agctgccgtg
    12361 atgggcgggg atgtaacgct tgtcctggga cctgtcagca taaagccccg ctccggggat
    12421 atcagcattg ttcccgtaac cactgccgca gagatgaagg aagccactgt ggcagcattc
    12481 agcacctgcg atattgccat cctggctgcc gcagtagccg atttcacacc ggagcacccg
    12541 catgagacca aggtgaaaag aggcgatgat gatatggtca tcaggctgaa acccactgaa
    12601 gacattgccg catgtctcgg gaagatgaag aggaaaggcc agttgctggc agggtttgcg
    12661 ctggagacag acaatgaact ggagagcgca aagggcaaac tccggaggaa aaaccttgac
    12721 ctgataatac ttaactcact gaaggatcag ggagccggat tcggccacga taccaaccgg
    12781 ataaccatca ttgacagtaa cgataatatt gataaatttg aactgaaaac gaaggacgag
    12841 gtcgcttcgg acatactgaa gaaaatcatc tcactgactc gctgaaagct tatgaagcaa
    12901 ccggttaaaa ctagacttct cgtagcggtg ctgctgatgg cgctgaccac cggcccggcg
    12961 gtgtcacagg agctttactg caacgtgcag gtgtcggctc agaagatcca gggttcgaac
    13021 cgtgaggttt ttcagaacat gcagcgtgac atttacgagt tcatgaacag catggtctgg
    13081 actgacaata tcttcagctt ttcagagagg atcgaatgca acctgctgat caaccttgat
    13141 gagcagcttt cggcagatga attccgcggc actatacagg tacagctcag caggccggtt
    13201 tacaacacca cctatgaatc cacagtgctc aacttcgtgg ataacaattt ccagttcagg
    13261 tatgtggagt tccagcctct ggagttcaat ccgaacagcc acacgagcaa ccttgtgtcg
    13321 gtgctggcct actacgccta ccttctgatc gggatggatt ttgacaccta ttcacagggt
    13381 ggcggaaccc cttatttcca ggttgccgaa aagattgtca ccaatgccca gaatgccccg
    13441 gaacagggat ggaagccata tgacggttca agaaacagga accggtactg gctggtaaaa
    13501 aatattctcg acagcgagta ttcgggtgtc aggcagttta tttatctata tcacctgaga
    13561 ggacttgacc ggatggagtc tgatatgact caggccagga ctgaaatata cgagagcctg
    13621 agacggttgc aggaggtcta caggcgccgc cctgacccgt tcatgtacta catgaccgtg
    13681 gtgcttgatg cgaaggcaga cgagattgtc aacatcttta cacagggttt tcccgaggaa
    13741 aagaacaggg tggtgcaggt gctgaccgag attgatcctg caaacgagac caagtatcag
    13801 aagatactga cttccaatag ttaaacccct gctgatgttg cgcagactca cagtaaggaa
    13861 ctatgccatc attaaggacc tgaacatgga gttcggtgag ggttttacca tcattacggg
    13921 tgaaaccgga gccggaaaat caattcttct gggtgccctg ggccttgtac ttggggagag
    13981 ggctgattcg tcggtgctgc tcagccagga tgataagtgc atcgttgagg catgcttcaa
    14041 cattggcggt tatgacctca atgaactgtt tgaagcgaat gagatcgatt atgatgagga
    14101 agctatattc aggcgtgaaa taacacctgc cggaaaatcg agggccttcc tcaatgacac
    14161 cccggtcaac cttacggttt taaaggagct tggcagcagg cttattgatg ttcattcgca
    14221 gcatgaaacc ctgctccttg gagccggttt cttccagatg cgtgtggttg acgcctatgc
    14281 agggatatca aacctgtccg gagattatgc ccgggcatgg aaagggtata ttgcagccag
    14341 gagggattac gatactgcaa tggcgggcct tgagaaggtg aaggctgatt ttgagtacta
    14401 ttcccatcag cttgaagagt tcagggctgt gaaacctgtt accggcgagc aggaggtgct
    14461 tgaaaaggaa caggagatgc tgtcaaatgc ctcggagatc aaggaggccc tggctgcggt
    14521 aaccgcagcg ctgggaggag aagaaatatc tgccctctca ctgctccgga cagtgaggac
    14581 aaaccttacc cggatctccg gctggtttcc cggggcggca gagctggaaa agagggtgga
    14641 agcacagctt atagacctga atgacatctc atatgaggct gaaaggctca atgacagggc
    14701 ttctgccgat ccgggcaggc ttgagtatgt tactcagagg cttgatgccc tctattcact
    14761 gatgcagaag caccgcagca gggatcttga tgagttgctg gctgttcaga acaggattga
    14821 ggaggctgtt gcagcctcat ccgatacgga cgagaagacg gaagagctca ggaagaggcg
    14881 tgatactgcg ctggcgcagg tgaattcact tgccggaaaa gtatctgaag ggaggatcaa
    14941 ggcagtggcg ccactctcac acgagataac cggaatgctt cacaggctgg ggatgcccca
    15001 tgcaaggttt gaagcttccg taaagcctct cccggcgccg ggaccctcag gaatggatga
    15061 aatagggttc ctcttcagcg ccaacaggca ggttgaacct gaagagctgt caaagtgcgc
    15121 ttccggcggc gagctctcaa gagtgatgct ctgcctgaaa tcggtgctgg ccaacagttc
    15181 gggactaccg gcaataattt ttgacgagat cgattcaggc gtctccggtg aggtggcctc
    15241 gatggtcggt tcgatcctgg cagacatggg caggagcatg caggtgatca atatcacgca
    15301 ccttccgcag gtggctgcca ggggcaggat acactaccat gtatataagg aggagggcga
    15361 ccactcaaca attacgaggg tcagattgtt aaatgacgat gaaagggtaa gggaggtggc
    15421 caggctcctc agcggaagca ctgtaactga tgctgcgatg cgcaatgccc gggaactgat
    15481 gaaggacaac tgagtctgct ggctgtaaaa gaaccgaaac aacaaaatca tgatcaagaa
    15541 tctgctcgag ggcaaaaaag ggatagtatt tggcgccctg aatgacaaat caattgcatg
    15601 gcatgttgct gagaaggctc atgcccaggg agcacgcctt gtgcttacaa acgcccctgt
    15661 ggcgatacgg atgggcacaa taggagaact ggctgaaaag accggcagtg aggtcatatc
    15721 tgccgatgcc accagcatga aggatctcga ggttctgttc acccggtcaa tggaaatatt
    15781 tgacggtaaa atagacttcg tgctgcattc catcggaatg tcgcccaatg tgcgtaaagg
    15841 tattccctat gcagaaagca gttatgaaaa catgcttaag accctcgata tttcggcatt
    15901 gagtttccac cgtattcttc agacagctta taagatggac gcaatcaggg agtggggctc
    15961 ggtgattgca ctcacctacg tggctgcaca gcgcaccctg gcaggttata acgacatggg
    16021 agacgccaag gcgcttcttg aatcaatagc caggagcttc ggatacatct acggcaagga
    16081 gaaacacgtc agggtcaata ccgtatctca atcgcccaca gccacaaccg caggagccgg
    16141 aattgccggt ttcgattcgc tggtcgattt ctccgacagg atgtcaccgc tgggcaacgc
    16201 cgacgcctcg gactgcgctg acgtctgcat aaccctcttc tctgacctga ccaggaagat
    16261 caccatgcag aacatattca atgatggcgg attctcaagc atgggtatga gcgagagggc
    16321 tatagaacag tataataaaa gcttcaggga gtgccctgat aagtaaaaat acccatactg
    16381 aagcagtata aataccgctt ttataagcgc actgccttac gggcagggcg ctttgtttat
    16441 ttttggttac ctaaaacaga cggccatgct attagctcta ttactgattg ttttctattt
    16501 tacctttcct cttgtattta tctggatgtg caaaaggtgg agccttcttc agaaacttgg
    16561 ggcaattgtc cttgcctacg gttttggact gataataggt actgcaggct tccttccgaa
    16621 gggaagcgaa gggtatcgtg aagccctgca gggagaggct acaatgagca ctggaaccct
    16681 tgacctgctt attgctgagg ggaaggctgt tccggaggac cacactgcga acaggattgc
    16741 cggaatacag gacaaggttt acaccgtttc actgctggtc gcatttccgt tgctcctgtt
    16801 ttcgcttaac ctgaagcgat ggctgaaata tgcaaaaaag ggttttgttt caatcggtct
    16861 tgcactggtg gcaggtcttg taatggtaac tgcaggattc tttatctgga aaaacagttt
    16921 tcctgacaca tggaaacttg ccgggatgtt tgaaggaatt tataccggcg ggaccccgaa
    16981 ctttgcagcc cttaagctga tactggatgt tgatgcagaa cggtttgtgg ttctgaacac
    17041 ctatgatatg attgtggggg cattcctcgt cctgttcttt gtgacgattg cacctgcaat
    17101 tttcagggct ttccttccca aattcaggga agaaaaaggg gtggctctgg atgaagagct
    17161 ggtcaaacag gagactgagg ggctcgacga tttttccgga atgcttgata aacaacgtat
    17221 atggcctctc ataagggctt tgcttatgag tattctgata gccggcgtcg gcgccgttgt
    17281 ctatttcctt ataccgcctg atttgaaaca gtacagaatg gccgggatgg tgcttacaat
    17341 taccaccctc ggaatccttg cttcgctgta caggcctatc aatcagataa agaagacatt
    17401 ccagctgggc atgtacctga tacttgtctt ctcgcttacc gtggcatcca tgtctgatct
    17461 gaggacaatg tttacaaagg ggatgcttga cctgatgttg ttcatctcct ggtgttactt
    17521 cggatccctt ttcctgcaca taatactggc caaaatcttc aggatagatg cggataattt
    17581 cctgattacg gctacggcat tcatattctc accaccgttt gttccattgg ttgcaaatgc
    17641 actgaaaaac aaggatgtga ttgtgaccgg tattgccggt ggcgttatcg ggtatacact
    17701 cggcaactat tttggtacca cacttgcatt tttcctgcag caattctgac ggaaccatct
    17761 aaaggtctaa aggtctaaag gtctaaaggt cggagattat tgcaattcac attaatcgat
    17821 actttcagac cccagacctt cagaccccag accttcagac cccagacttt cagaccccag
    17881 accttcagac ccgagacttt cagaccccag acctcagacc ttcaggcctc taaaacaaat
    17941 agttgagccc tatatagggt ttgctgagaa aatcaagtta agcaataaag caacctgata
    18001 tttctact
//
LOCUS       DAOPXU010000057        16911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_3593_length_16911_cov_11.852753, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000057 DAOPXU010000000
VERSION     DAOPXU010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16911
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3593_length_16911_cov_11.852753"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PKK03_07265"
     CDS             <1..765
                     /locus_tag="PKK03_07265"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HNX84238.1"
                     /translation="LHYYTTNWNNKGSATKFTEKDYFETVERCLNVEKALDRHIAVMD
                     SYDPARRVALVVDEWGTWWDVEPGTNPGFLYQQNTMRDAIVAGLSLNYFNERCERVKM
                     ANIAQTVNVLQSLILTDGDKMLLTPTYHIFEMYKVHHDATLLPLHLNCGNYSLDGREV
                     PSLSASASKDAKGAVNISLVNVDPSAESEVLIDLRGGEYSGVTGRILSGAELNAHNTF
                     DKSETVRPVTFTGARLKKNILSLKVPPASVVVLTVN"
     gene            833..1963
                     /locus_tag="PKK03_07270"
     CDS             833..1963
                     /locus_tag="PKK03_07270"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HNX84239.1"
                     /translation="MKRERLILLTAVALAFIGLCSCGNNKAKEMVTTENFGSFEGQDV
                     SLFTLKNKQGDIVKLTNYGAAIVEINVPDRNGDKENVTFGYETLDGYLAGDAYFGKVV
                     GQFANRVAKGKFTLDGVEYTLALNNGPNSLHGGPTGWHSRAWTAEVLKGTDFPAVKFT
                     YSKPDMEEGYPGNVVAEVVYTWTDDDELIMDYKVTTDRRTVINITNHAYFNLHGTGNG
                     DILDHEALIRASAFTPVDSTLIPTGELRPVEGTPFDFRTPHLIGERIGEAYDQLVLGG
                     GYDHNFVLDNVEQVDAEVYDPSTGRVIQVITDQPGVQLYTGNFLDGTQTGRGGKVYQY
                     RSGFCLETQHYPDSPNQASFPPVILTPEEPFLSSTTYKFGVR"
     gene            complement(2060..3430)
                     /locus_tag="PKK03_07275"
     CDS             complement(2060..3430)
                     /locus_tag="PKK03_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNX84240.1"
                     /translation="MKARLLLPAVLLTLLLTGCGQSGWHDLFNGKDFTGFVQKGGQAQ
                     FTVEDGVMVGISTPDTPNSFMCTSEEYSDFILEFEVKADTILNSGVQVRSHSTENGDR
                     TLVYGYQVEIDPTDRGWSGGIYDEARRGWLYPVTPNNVAAVRSFDRHGWNSYRVEAIG
                     NRIRTWINGIPVADLADDADSSGFIAFQVHAVSRELAGTKVMWKNIRIATENLEKLAL
                     PDTTKIFQANFIPNTLTEREKADGWKLLFDGTSSAGWRGAHKDAFPESGWVIENGCLK
                     VLASGGAESQGGGDIVTIDQYGNFDLFFEFMITEGANSGVKYFVSEAYPTAGSAIGLE
                     YQILDDQRHPDAKMGRDGNRTLSSLYDLIPAANKRFNGVGQWNAAHIVSVNGHVEHWL
                     NGFKVLEYERGSEEYRKLVSESKYKVFAGFGEAEHGHILLQDHGNEVAFRSIKIRQEA
                     RGNRQW"
     gene            complement(3441..4787)
                     /locus_tag="PKK03_07280"
     CDS             complement(3441..4787)
                     /locus_tag="PKK03_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX84241.1"
                     /translation="MSKRRDFVKNSLLASAGIAGLGSVASLYGATGRRVNPSDKIRVA
                     LIGGRNMGWSDLETFLKFPQVECVSICDIDNEWLYKRAADTEKISGKRPSQIVKDWRR
                     VMDNPDVDAVIIGTPDHWHCLQAISAMEAGKDVYCEKPLANSIAECDAMVRAARNNKR
                     VVQTGQWQRSDPHWQDAVAFIHSGKLGRIRTVKVWAYMIWKKTLPVQPDGTVPAGVDY
                     DMWLGPARHRPFNPNRFHYNFRYFWDYAGGLMSDWGVHLLDYALLGMKADLPKAVYTA
                     GGKFAYPGDAMETPDTLLASYVYDDFTISWDHACGIKDGPYGREHGLAFIGENGTLLI
                     DRNGWEVIPEVDSAPRMEAVPLMKKSEQIEGQAVAGGGLASHIANFLDCISTRNLPNA
                     DVEIGARVAKMTHLANVSCRLGKPLTWDNPSNMFGEADANALIMPYYNEPWKFPKY"
     gene            5021..7651
                     /locus_tag="PKK03_07285"
     CDS             5021..7651
                     /locus_tag="PKK03_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNX84242.1"
                     /translation="MKRPAALLFAVLLFVSVSAQQYPFLDTGLSFEKRVDDLVSRMTV
                     EEKAAQLLYTAPAITRLGIPEYNWWNEALHGVARAGHATVFPQSITFANSWDEELMLD
                     VANAISDEARAKHHEFVRRGDRGIYHGLTFWSPNINLFRDPRWGRGQETYGEDPYLTG
                     RLGLRFVQGMQGSDPKYLKTIATAKHYAVHSGPEPLRHKFNAVISERDLRETYLPAFR
                     TLVKEGKVYSVMGAYNQFRDHPACANDELYGILRNEWGFDGYIVSDCWAVSDFYNFQG
                     YAAGPVEAAAMALKAGTDLECGVDYNHLTEALKKGLITEADIDRSARRVMLARFRLGM
                     FDPDSIVDYAQIPYQANCSDYNRSLARKAARESIVLLKNSNGTLPLSRDIRTVAVIGP
                     NAASHEALVGNYNGIPKDPVSLLEGIKAKLRPDAAVLYAEGSDLATGMHNLVPIPSRY
                     LRTPDGKQGVLGAYYTSREMKGEPLFTRTDDMIDFYWDNDSPHSLMPVNDFGIHWVAD
                     LVPPVTGTYYLGTWGSSGYTIILEGDTLLSFYNEHHAAIREVPMDLRAGEKYRVEVLY
                     RNHGGDADMELLWSMPRDDMKANAVEAASQADAVIVALGLSPRLEGEELNLKLEGFEG
                     GDRTTLGLPAIQEELLAALLATGKPVVVVLMNGSPVSSLQAHEKATAVLLAGYPGVEG
                     GNAIADVLFGDYNPAGRLPVTYYRSVDQLPPFEDYDMDNRTYRYFRGTPLYPFGYGLS
                     YTTFAYSDLVVPAKTVAGTEVKVSVTVANIGKMTGDEVVQLYLTDEKATTPRPLRQLE
                     GFDRITLRPGESRRVEFTLKPEQFSMFNNKDTRVIEPGWFTVSVGGGQPGTAVTGAEG
                     HAQAMTARFRLTGKEVVMKE"
     gene            7752..8369
                     /locus_tag="PKK03_07290"
     CDS             7752..8369
                     /locus_tag="PKK03_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNX84243.1"
                     /translation="MTAIEKNKRRTGWVVILTAVTMVVEIIAGLLTGSMALLADGIHM
                     GSHVLAIGLSWAAYVLVQKLSANPSFTGSKEKILTLSGYTSGLILLIFAGMIIAGAVG
                     RFINPRIIEYREAIMIAFIGLGVNILSAFLLHHDHEEADNNIRAAYLHVLADAITSVA
                     AILGLVTAMIWGLPYVDAIAALISSAVIIRWSLGLLRDSGRELIA"
     gene            8722..10092
                     /locus_tag="PKK03_07295"
     CDS             8722..10092
                     /locus_tag="PKK03_07295"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methyltransferase"
                     /protein_id="HNX84244.1"
                     /translation="MLPPEFLERIESQAYIDARGLAGALGGPSAASVRVNRLKWPHNV
                     TGYDAVEWEPDGFYLPGRPLYTSDPLFHSGVYYPQESSGMFTGEMFRQVTAGQGGLRV
                     LDLCGAPGGKSTHIASLLGDDGLLVANEVIRSRSAILAENITKWGTGNVIVTNADPSR
                     FVQLPGFFDVVVADTPCSGEGMFRSAVAVREWSLQNANLCCERQRRIIMEVWPALKPG
                     GMLIYSTCTFNPAENEENVEWIREGTGAEPVMISLPPGSPVVPVRYGGTEGYGFHPGR
                     VRGEGFFIAALRKPVRPVQGERVSGGRKSVLKPSPEAFERTMSLADFDRGRVIMHEGR
                     IIALAADAGLFGFIEARLPVIKAGTMIGELKKNDLVPAHDLAMSVRQRQEAWTRHSLS
                     REEALVFLRLESMNGSSMPAGRVLLTYRDVPLGFVKNLGNRVNNGYPQGWRIRMEKET
                     GITEIL"
     gene            10186..11856
                     /locus_tag="PKK03_07300"
     CDS             10186..11856
                     /locus_tag="PKK03_07300"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNX84245.1"
                     /translation="MKTDIEIAQSASIRPVTEVAQKLGLKEDDLELYGKYKAKLPLSL
                     IDEKKAARAKLILVTAITPTPAGEGKTTTSIGLAQGLNQLGKRATVVLREPSLGPVFG
                     IKGGAAGGGYSQVIPMEDINLHFTGDLSAVEKAHNLLAAMIDNNIQSSEGNLGIDPRT
                     IAWKRVMDMNDRALRDVVIGLGGTAQGVPRETGFDITAASEVMAILCLATSISDLKER
                     LGNIFIGYTYAGKPVFARDLRAQGAMAALLKDALKPNLVQTLEGTPAIIHGGPFANIA
                     QGTNSILATKMGLSLSDYVVTEAGFASELGAEKFFNIKSQVGKLKTNAVVIVATIRAL
                     KYHGGVRLSELANENLEALKKGFENLDKHIENMHYYGFRPVVAVNRFSSDTEAEIELL
                     KKYCEEHNVRVAVNSAWADGGKGAVELANAVIEASDACTDCFTPLYDLAWPLEKKIET
                     ITTLMYGARNVEYTLEAKEQLKKISELGLDSLAVCIAKTQKSLSDDQARRNRPRDFTI
                     RIRKVELSSGAGFIVPIAGSIMRMPGLPAIPSAEKIDIDDRGQISGLF"
     gene            11886..12437
                     /locus_tag="PKK03_07305"
     CDS             11886..12437
                     /locus_tag="PKK03_07305"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNX84246.1"
                     /translation="MLAGGRASRFGGRNKALIPVDGVTVISRTISLLEPLFSEMIVAG
                     WPPDDPLPGDVILVHDNYGGVGPLAGIEAALRVVRSPVLFVFGGDMPWLSEELIRAQA
                     NEFLNNPPDILVARSGGLAEPLHSLYSRKIHPALVGHIEGGGSNAIIDFYKLADTRYF
                     DLPLTREARKALTNINRPEDLLI"
     gene            12483..15719
                     /locus_tag="PKK03_07310"
     CDS             12483..15719
                     /locus_tag="PKK03_07310"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNX84247.1"
                     /translation="MRNSILLMIVAAALLSSCRKYHDYSKTEWTEKDLPEWEDLTVNT
                     INTVTPHASMVSHPDNSSALKAKWRESVNVLSLDGKWKFNYSPAPAGRPYWFFREDFD
                     TRQWGEIEVPSTWEREGHGTAYYVNSGYTFPVNPPFINHSDNPVGSYKRSFTIPSGWK
                     EKEVFLNFDGVSSAFNVWINGEKVGYSEDSKTTAEFNITPFLRKGKNNIAVEVYRYSD
                     GSYLECQDFFRLSGIQRSVWLHARPKTYIRDYFAVPTLVNEYTDGLLNLTVELAGNER
                     RPSEMRLEAGLFDGETKIFAETRDVEAFDSLAVVELTAEVPDVNPWSAESPSLYTLVI
                     TLADSRGRVLESVSSRIGFRTSEVKGGRFLLNGKAIYLKGTNMHEHDPVRGHTIDEAL
                     MLKDIRLMKMNNINAVRTSHYPQPERFYELCDEYGLYLIDEANIESHGIGYHPDITLA
                     NRPEWLGQHMFRTVRMVERDKNHPSVIIWSLGNEAGDGQNFVETYKWIKGRDESRPVQ
                     YERAEKSTRTNERHTDIWCPMYPPIERVESYALSEGQPGFDRPYIMCEYAHSMGNSTG
                     NLQDYWDVIEKYGILQGGFIWDWVDQGLLETSEEGEEYFAYGGDYGEVGSPSDGNFCC
                     NGLVGPDRTPHPALTEVKKVYQYVGFNPADLTRGLVTISNKYDFTNLSYFTINWEVLA
                     NGILFRSGNLRQISLEPGDSLTVEIPWGDIAPAPDTEYHLNLHVSRSDEWGIVPQDHM
                     YASEQMLLPFHAAAKTAASSVASVAAGASGEESLALIDTRTEGSDLTFAGEGFEVVFD
                     MAAGVMKSYRVAGQELIMSGPRPDFWRAPIDNDYGNGMEKRNAPWREAGGRAVLKSAR
                     ISQPGMGSAGVEFTFDIPGDGGKKIAGLVTGYTVHAGGSVEVSFNLTKTDMSLEEIPR
                     VGMQMLLPGRFVDLSWFGRGPHENYADRKTSAFAGVYESSVADQYVPYVRPQENGYKT
                     DTRWLNVTDGSGKGIRFEGEPMFSFAAMNYLHEDFESQGSLAGYRPDAKLANRHISDV
                     IPRDLVRVNIDYGQMGVGGDNSWGARTHPQYCLTAREYRYGFRMVPLK"
     gene            complement(15773..16852)
                     /locus_tag="PKK03_07315"
     CDS             complement(15773..16852)
                     /locus_tag="PKK03_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84248.1"
                     /translation="MKIRLFTLSLLLLGTMSLQAQRRMLSVPELPGYVTLKCDFHMHT
                     VFSDGNVWPIYRVGEAWRDGLDVISITDHIEYQPHKQYIPVDHSAAWKIASATAADYN
                     IILVKGSEITRKMPPGHLNALFISEPDSLVKDDFMKVIEAAVAQGAFIQWNHPGWKSQ
                     QPDGIPRMYDVHRELIEKGWLHGIEYYNHVEYYPLVMDMCRDNKLAVMGNSDFHDVIS
                     EGYAAPEYSHRPMTLVFAKERTGESLKEAMFARRTAVWYGDNLAALEEIAAPLFRSVI
                     TAGAPFKDDGTRIWFELCNSSEIPMKLSGGPEGAPATFTVPARGMVVVRADRKYLSQP
                     LVYNVDNIVTGSNSMLKVEISPAKK"
BASE COUNT         3982 a         4234 c         4711 g         3984 t
ORIGIN      
        1 ctgcactact acacaaccaa ctggaataat aagggatctg ccacaaaatt caccgagaag
       61 gattattttg agaccgttga gcgctgcctt aatgtggaga aagctcttga caggcatata
      121 gccgttatgg attcatatga tcctgcccgc cgggttgcac ttgttgttga tgaatggggc
      181 acatggtggg atgttgagcc gggaaccaac ccgggtttcc tttatcagca gaatacaatg
      241 cgcgatgcca tcgtggcagg tctttcgctg aactatttca acgagcgctg tgaaagggta
      301 aagatggcca atatagccca gacggtcaac gtgctgcaat cacttattct tactgacggc
      361 gacaaaatgc tgttgacccc gacatatcac atcttcgaga tgtacaaggt tcaccatgac
      421 gccacacttc tcccgctgca cctcaactgc ggcaactact cactggacgg gagggaagtt
      481 ccatcgctta gcgcttccgc ttctaaagat gcaaagggcg ctgtgaacat ctctctcgtt
      541 aacgttgacc cttcagcgga gtcagaagta cttatagatc ttcgtggcgg agaatactcc
      601 ggggtgactg gaaggatcct cagcggtgcg gagctgaatg cccacaacac ctttgacaag
      661 agtgagactg tccggccggt tacttttacc ggggcccggc tgaagaagaa tattctcagc
      721 ctgaaagtgc cgccggcatc agtggttgta ctgactgtaa actgagacca tcaggctggt
      781 atcattaagg aaggcataaa ataaatttgc tcataatttt acattacaag aaatgaaaag
      841 agaacgactc attttactaa cagctgtggc gcttgccttt atcgggctgt gctcatgcgg
      901 aaacaacaag gcaaaagaaa tggtaactac tgaaaatttc ggttcattcg agggacagga
      961 tgtatccctg ttcaccctga aaaacaagca gggagatatt gttaagctca ccaattatgg
     1021 tgctgcgata gttgagatca atgtgcctga cagaaatggc gacaaggaaa atgtgacctt
     1081 cgggtacgag acacttgacg gctatcttgc aggcgatgca tatttcggca aagttgtggg
     1141 ccagtttgcc aaccgcgttg caaagggaaa attcaccctt gacggggttg agtacaccct
     1201 tgctctcaac aatggtccca attcacttca cggcggtccc accggctggc acagcagggc
     1261 ctggacggcc gaagtactga agggcactga ctttccggca gtgaagttca cctacagcaa
     1321 gcctgacatg gaagagggtt acccgggcaa cgtcgttgct gaggttgtat acacctggac
     1381 cgacgatgac gaacttatca tggattacaa ggtcacaact gaccgcagga cagtgatcaa
     1441 cattacgaac catgcatatt tcaacctgca cggcacaggc aatggtgaca tccttgatca
     1501 cgaggcactc atcagggcat cggcattcac gccggttgat tcgaccctca ttcccaccgg
     1561 ggaactgagg cctgtggaag ggactccgtt tgatttccgc actcctcatc tgattggcga
     1621 gaggatcggt gaggcatatg accagctcgt cctgggcgga gggtatgacc acaactttgt
     1681 actcgacaat gttgagcagg ttgatgccga ggtctacgat ccgtcaaccg gcagggttat
     1741 ccaggttatc acagatcagc cgggtgttca gctttacacc ggcaacttcc ttgacggcac
     1801 ccagaccggc cgcggaggca aggtctacca gtaccgttcg ggattctgcc ttgagacgca
     1861 gcattatcct gacagtccaa accaggcatc ctttccgcca gtcatcctga ccccggagga
     1921 gcctttcctg tcaagcacta cctataagtt cggggtaagg tagaaggtct gaaggtctga
     1981 gggtctgagg gtccgggaaa attaaggctt agtctcataa ataataacag gcagtaatta
     2041 actctactgc ctgttatttt taccactgcc tattgcctct tgcctcttgc cttatcttga
     2101 tgctgcggaa tgcgacctca ttcccgtgat cctggagcag gatgtgccca tgttccgctt
     2161 caccgaagcc ggcaaaaacc ttgtatttgc tttcgctgac aagcttgcgg tactcctcgc
     2221 tgcccctctc gtattccagc accttgaaac cgttcagcca gtgctccaca tgaccgttca
     2281 ccgatactat atgtgctgca ttccactgac ccacaccgtt gaaacgcttg ttggcagcag
     2341 gaatgagatc atagagtgaa gagagagtac ggttgccgtc acggcccatc ttcgcgtcgg
     2401 ggtgacgctg gtcatcgagt atctggtact caagtcctat ggctgagcct gccgtcggat
     2461 aggcttctga tacgaaatac tttaccccgc tgtttgctcc ttcagtgatc atgaactcga
     2521 agaacaggtc gaagttgccg tactgatcaa tggtgacgat gtcaccaccg ccctgtgact
     2581 cggcacctcc ggaagcgagt actttaaggc agccgttctc aatgacccat cctgattccg
     2641 ggaaggcatc cttgtgagcc cctctccatc cggctgaaga agtaccgtca aacagcagct
     2701 tccatccatc tgctttttcc cgttcagtga gggtgttggg gatgaaattt gcctggaata
     2761 tttttgtcgt gtcgggtaac gcaagttttt cgagattttc agtggctatc ctgatgtttt
     2821 tccacatgac cttcgtcccg gccagttccc ggctcacggc atgcacctgg aacgctatga
     2881 accctgaaga atcggcatcg tcagccaggt cagcgacagg gataccgttg atccatgtcc
     2941 tgatcctgtt gccgattgcc tccacccggt agctgttcca gccatgccgg tcaaatgatc
     3001 tgacggctgc gacattgttc ggtgtgacgg ggtatagcca tccccgtcgt gcttcgtcat
     3061 agattccgcc gctccagccc ctgtctgtgg gatcaatttc cacctggtat ccgtaaacca
     3121 gggttctgtc accgttttcg gtgctgtgac tcctgacctg tacgcccgag ttgagaatgg
     3181 tatctgcctt cacttcaaac tcaaggataa aatctgagta ctcttctgaa gtgcacatga
     3241 agctgttcgg ggtgtcaggg gtggatattc cgaccatcac accgtcttca accgtgaatt
     3301 gtgcctgtcc gcctttctgg acgaatcctg taaagtcttt tccgttgaag aggtcatgcc
     3361 agcctgactg accgcatccg gtcagcaaaa gagtcagaag aacagcaggg agaaggagtc
     3421 ttgctttcat agttttatga ttagtatttg ggaaatttcc acggttcgtt gtaataaggc
     3481 attatcagag cattggcatc agcttcgccg aacatgtttg atgggttgtc ccaggtcagg
     3541 ggtttgccca gcctgcatga gacgtttgca aggtgtgtca tcttggcaac ccgggctcct
     3601 atctcaacat cagcgtttgg cagattgcgg gttgaaatgc agtcaaggaa atttgcgatg
     3661 tgtgaggcga gtccacctcc ggccacggcc tgcccttcga tctgctcgga tttcttcatg
     3721 aggggtacgg cttccatgcg cggtgctgaa tccacctccg ggatgacctc ccagccgttg
     3781 cggtcaatca gcagggtgcc gttctcgccg atgaaagcca gaccgtgttc acgtccgtaa
     3841 gggccgtcct tgattccgca ggcgtggtcc cagctgatgg tgaaatcatc ataaacatac
     3901 gaggccagga gcgtatcagg tgtctccatg gcatcacccg gataagcaaa tttgccaccg
     3961 gcggtataaa cggctttcgg gaggtcagcc ttcatcccaa gaagggcata gtcgagcagg
     4021 tgtacccccc agtctgacat aagtccgcct gcataatccc agaaatagcg gaagttatag
     4081 tggaaccggt tcgggttgaa cggtctgtgt ctggccgggc cgagccacat atcgtagtca
     4141 actccggcag gtacagtacc gtcaggctgc acgggcaaag tctttttcca gatcatgtat
     4201 gcccagacct ttaccgttct gatccgtccc agtttacctg agtggataaa ggcaacagca
     4261 tcctgccagt ggggatcgct acgctgccac tggcctgtct gtaccacgcg cttattatta
     4321 cgggctgccc tgaccatggc gtcacactcg gctatggagt tggcgagcgg cttctcacag
     4381 tagacatctt tgccggcctc catggccgag attgcctgca ggcaatgcca gtggtcgggg
     4441 gtgccgatga taacggcatc cacatccggg ttgtccataa cccttctcca gtccttcaca
     4501 atctgtgacg gccgcttccc ggagatcttt tccgtatcag cggctctttt ataaagccat
     4561 tcgttatcga tgtcgcaaat ggagacgcac tccacctgtg gaaatttcag gaaggtctcg
     4621 aggtctgacc agcccatgtt acggccgccg atcagcgcca ccctgatttt gtctgagggg
     4681 ttcacccttc ttccggttgc tccgtagagt gaagccactg atcccaggcc tgcaattccg
     4741 gctgaagcca ggaggctgtt tttcacaaag tctcttctct tgctcatgat ctgattgctg
     4801 tttgggagcc cggcaaaaag ccgggtctgt tcccaaaggt aataattcat atcaaaggat
     4861 aaacaaagga gatgagctcc ctttcaggag agctcaccat aatttaactt gccccggctg
     4921 ttagctgaag ttattgtgtg aatttcgccc gtaagggcag gcggggtatc agaataaacc
     4981 caattagtga tatctttatc agtataaaaa tatccaaaga atgaaaagac cagccgccct
     5041 tctgtttgca gttttgctgt ttgtgtcagt cagcgctcag caatatccgt ttcttgatac
     5101 cgggctttca tttgaaaagc gggttgatga ccttgtctcg cgcatgactg tcgaggagaa
     5161 ggcagcgcag cttctttaca ctgctcctgc cattacgcgg ctgggcattc cggagtacaa
     5221 ttggtggaat gaggcactgc acggggttgc cagggcgggg cacgcaactg tttttccgca
     5281 gtcgattact tttgcaaaca gctgggatga agagctgatg cttgatgtag ccaatgccat
     5341 atcggatgag gcaagggcaa agcaccacga atttgtgaga agaggtgacc gtggcatata
     5401 tcacgggctg accttctggt cgccgaacat caatctcttc cgtgatcccc gctgggggag
     5461 gggtcaggag acttacggag aggatccgta ccttaccgga aggctggggt tgaggtttgt
     5521 gcaggggatg cagggatctg atccgaagta tctaaaaacc atagcaaccg cgaagcatta
     5581 tgcagttcat tcgggccctg agcccttaag acataagttc aatgcggtga tcagcgagcg
     5641 tgaccttcgc gagacatatc tgcctgcgtt tcgcaccctg gtgaaggaag ggaaggtcta
     5701 ctcggtgatg ggtgcctaca accagttcag ggaccacccg gcatgcgcca atgatgagct
     5761 ttacggcatt ctgcgaaatg agtggggttt tgacggttac attgtctcag actgctgggc
     5821 ggtatctgat ttttacaatt tccaggggta cgctgcgggc ccggtcgaag cggccgcaat
     5881 ggctctgaaa gcgggtactg acctcgagtg cggggtagac tacaaccacc tgacggaggc
     5941 cctgaagaag gggctgataa cagaggcaga tattgaccgt tcggcacgaa gggtgatgct
     6001 ggccaggttc aggctcggga tgttcgatcc tgacagtatt gttgattatg ctcagatacc
     6061 atatcaggca aattgctcgg attacaaccg gtcacttgcc aggaaggcgg cccgggaatc
     6121 aattgttcta ctcaagaaca gcaacggaac cctccccctg agcagggata tccgcactgt
     6181 ggctgtaatc ggtcccaacg cagccagcca tgaggcgctc gtgggcaatt acaacggcat
     6241 tccaaaggac ccggtcagcc tgcttgaggg aataaaggcc aaactcaggc ctgatgctgc
     6301 ggttctatat gcagagggga gtgacctggc gaccggaatg cataacctgg taccaatacc
     6361 atccagatat ttgcgtacac ctgacggcaa acagggagta ctgggcgctt actacacgtc
     6421 acgtgaaatg aagggcgagc ctcttttcac ccgcaccgat gacatgattg acttctactg
     6481 ggacaatgat tcacctcatt cgctcatgcc ggtaaatgac ttcggcattc actgggttgc
     6541 cgatctggtt ccgccggtga caggcacgta ttatcttggc acatggggtt catcgggtta
     6601 taccattatc ctcgaaggag ataccctcct ttccttctac aatgaacatc atgcagccat
     6661 acgggaagtg ccgatggatc tcagggcggg agagaaatac agggttgagg tactctaccg
     6721 caaccacggc ggtgatgccg atatggaact cctctggagc atgccgcgcg atgacatgaa
     6781 agctaacgcg gtggaagcag cctcacaggc tgatgcagta attgttgcac tgggtctctc
     6841 tccccggctc gagggggagg agctgaacct gaaactcgaa ggtttcgaag ggggagacag
     6901 aaccaccctc ggcctgcccg ccatccagga agagctgctg gcagcgctgc tcgccaccgg
     6961 gaagccggtt gtggtggtgc tgatgaacgg cagcccggtt tcttccctgc aggcccatga
     7021 aaaagccacg gctgtgctac tggcaggtta ccccggcgtg gaggggggca acgcgatagc
     7081 tgatgtcctc ttcggtgatt acaacccggc cgggcgactg cctgtcacct actacaggtc
     7141 ggttgaccag ctgcctcctt ttgaagatta tgatatggat aaccgcacct acaggtattt
     7201 caggggaaca cccctttatc cttttgggta cggattgagt tataccacct ttgcctattc
     7261 ggacctcgtg gtgccggcaa agaccgtcgc cggtacagag gtaaaggtga gtgtgactgt
     7321 cgccaacatc gggaaaatga caggcgacga ggtagtacag ctttatctga ctgatgagaa
     7381 agccactacg ccgagacctc tcagacagct tgaagggttc gaccggatta ccctgaggcc
     7441 cggggagagc agaagggttg agtttaccct gaaaccggag cagttctcaa tgttcaacaa
     7501 taaggataca agggttattg agcccggatg gtttaccgtc agtgtgggag gagggcagcc
     7561 ggggacggcg gtgactggtg ctgaaggtca tgctcaggcc atgactgcaa ggttcaggct
     7621 tacagggaaa gaggttgtta tgaaagaata acatcggcgg cgaaattgac cagccggatg
     7681 gcggctcatt gtcaggggaa taaattctta aacggcttaa acaataggag aggcctgtcc
     7741 gtttattagc tgtgacagcg attgaaaaaa ataaaaggcg caccggctgg gtagtcattc
     7801 tcaccgcagt gacaatggtg gtggagataa ttgccgggtt gcttacaggc tccatggccc
     7861 tgctggccga cggcattcac atgggttcgc atgtactggc catagggctc agctgggctg
     7921 catacgtgct tgttcagaag ctctccgcca acccctcgtt taccggcagc aaggagaaga
     7981 tactgactct ttcaggttac accagcggac tcatacttct tatttttgcc ggaatgataa
     8041 ttgccggcgc cgtggggcgg ttcataaacc cgcggatcat tgagtaccgt gaggccatca
     8101 tgatagcctt tatcggtctc ggggtgaata tactcagcgc ctttctgctg catcatgatc
     8161 atgaagaggc tgataacaat atcagggcgg cataccttca cgtgctggct gacgcaatta
     8221 ccagcgtggc agccatcctg ggactggtca ctgcaatgat ctggggactg ccctatgtag
     8281 atgcaatagc agccctcatc agttcggcag tcatcatcag gtggtcactc ggactgctaa
     8341 gagactccgg cagagagctt atcgcctgag tttactaaaa aatgttaaat aggctaaaca
     8401 tgagatatgt catcataagc acagtgtttg agccttacat ttgcaagtac ctaattagtg
     8461 taactcttac actttagtct tcatacggat ttgttgcgac aaataccgtc ctattagaca
     8521 gtttaggttt taggggttat tttaagtgcc gccccgggtt accggggcgg cattctttgt
     8581 gtgtgcccgg catgggcgat aactataggg tgagagtccc gagcgcgccc tgacaatggg
     8641 aggcgttagc tgaagacagg ggtgattatc agtcagccgg attgatttca atggttatct
     8701 ttgtccggaa acagtaaaat gatgttgccc ccggagtttc ttgagcggat tgaaagtcag
     8761 gcctatattg atgcccgtgg cctcgccggg gcgcttggcg ggccgtctgc cgcctcagtg
     8821 cgggtcaaca ggctgaagtg gcctcacaat gtaaccggct atgatgcggt ggagtgggag
     8881 cctgatgggt tttatcttcc tggccggccc ctgtatacca gtgatccgct gtttcattca
     8941 ggggtctatt accctcagga gtcatcgggg atgtttaccg gagagatgtt ccggcaggtg
     9001 acagccgggc agggcggtct ccgggtgctt gatctctgcg gtgccccggg cggaaagagc
     9061 acccatattg cctcccttct cggagatgac gggctgctgg tagcgaatga agtcatcagg
     9121 tcacgttctg ccattcttgc agagaacata accaagtggg ggacaggcaa tgtcatcgtg
     9181 acgaatgctg atccttcgcg ctttgtgcag cttcccggtt tctttgatgt ggtggttgct
     9241 gatacgccct gttcgggcga agggatgttc cgctcagccg ttgcagtcag ggaatggtca
     9301 ttgcagaatg caaatctctg ctgcgaacgt cagaggagga taattatgga ggtatggcct
     9361 gcacttaaac ccggaggcat gctgatctat tcaacctgca cattcaaccc tgctgaaaac
     9421 gaggaaaatg tcgaatggat aagggagggt accggtgctg agcctgtcat gataagcctt
     9481 cctccgggca gtccggtggt accggtgagg tacggaggta cagagggtta cgggtttcat
     9541 cccggcaggg tcaggggaga aggatttttc attgccgcac tacgaaagcc tgtgaggccg
     9601 gttcaagggg agcgggtgtc cggcggcagg aaaagtgtct tgaagccttc gccggaggct
     9661 tttgaaagga ccatgtccct tgcagacttt gaccgtggaa gggtgattat gcacgagggc
     9721 cgcatcattg ccctggcggc ggatgccggc cttttcggat tcattgaagc aaggctgccg
     9781 gtaatcaagg cagggacaat gatcggtgaa ctgaagaaga atgacctggt accggcgcat
     9841 gacctggcaa tgtcagtgag gcagaggcag gaagcatgga cgaggcactc actttcacgt
     9901 gaggaggctc tggttttcct caggctggag tcgatgaacg gctcctcgat gcccgcgggg
     9961 agagttctgc tgacctatcg tgatgtgcct ctgggcttcg tgaagaacct gggcaacagg
    10021 gtcaataacg gttatcccca ggggtggcgc atcaggatgg aaaaagaaac cggaataact
    10081 gagattttat aaatcattct ttgttgaact aaactatcac aacagataat tgttcttgtg
    10141 aaaaggaatt aaaacttaat tttatcccaa acaattgttt cccctatgaa aacagacatc
    10201 gagatcgctc agagtgcctc aataaggcct gtcaccgagg ttgcacagaa gctgggactt
    10261 aaggaggatg accttgaact gtacggaaag tacaaagcca agctgccgct ttctcttatt
    10321 gacgagaaaa aggcggcgcg tgcaaagctc atacttgtta cagcaatcac tcccacgcct
    10381 gccggtgaag gaaagaccac cacttcaatc ggactggcac agggtctcaa ccagctgggt
    10441 aaaagagcca ccgtggtgct gagggagccg tctctgggac ctgttttcgg cataaaggga
    10501 ggggcagccg gtggcgggta ttcacaggtg atacccatgg aggacataaa cctccatttt
    10561 accggtgatc tgtctgccgt tgagaaagct cacaaccttc tggctgcaat gattgacaac
    10621 aacatacagt cgtcggaggg aaaccttggc attgatccgc ggaccattgc atggaagagg
    10681 gtaatggata tgaatgaccg tgctctccgt gacgtggtga ttggtctggg cgggacggcc
    10741 cagggtgttc ccagggagac aggatttgat ataacggctg cctccgaggt gatggcaatt
    10801 ctgtgcctgg caacgagcat cagtgatctc aaggagcggc ttggaaatat tttcatcggt
    10861 tacacatacg caggcaaacc ggtttttgcg cgtgatcttc gtgcccaggg tgctatggct
    10921 gcactcctca aggatgccct gaagcccaac ctggttcaga cacttgaagg cactcccgcc
    10981 attattcacg gcggtccgtt tgcaaacatt gcccagggta ccaactccat actggcaaca
    11041 aagatggggc tctcgctcag tgactatgtg gtcaccgagg caggttttgc cagcgagctg
    11101 ggcgccgaga agtttttcaa catcaagtcg caggtaggta agctgaaaac caacgcggtt
    11161 gtgatagttg ccaccatcag ggctctgaag tatcacggag gggtcaggct ctccgaactt
    11221 gccaatgaaa acctcgaagc actgaagaaa ggttttgaga atcttgacaa gcacattgag
    11281 aacatgcatt attacgggtt caggccggtt gttgcggtca accgtttcag ttccgataca
    11341 gaggctgaga ttgaactgct taagaaatac tgcgaggagc acaatgtcag ggtggctgtc
    11401 aattcagcct gggctgacgg gggaaaggga gccgttgagc ttgccaatgc cgtcattgaa
    11461 gcctccgacg catgcactga ctgttttacc ccgctatatg accttgcgtg gccgctggag
    11521 aagaagattg agacaatcac caccctcatg tacggtgcaa gaaatgtgga atacaccctg
    11581 gaggcaaaag aacagctcaa aaagatttcc gagcttggcc ttgacagcct cgcagtgtgc
    11641 atcgccaaaa cccagaaatc gctctctgat gaccaggcca ggcgcaacag gcccagggat
    11701 ttcaccatcc gaatccgtaa ggtggaactg tcatcaggag ccggattcat tgtgccgata
    11761 gccggatcca tcatgcgcat gccgggtctg ccggcaatac cctcggcaga gaagatagac
    11821 atagacgaca ggggacagat tagcggatta ttctgatcaa ggcatttcct gaaatatcgg
    11881 cagccattct tgcaggcgga cgggcttccc gcttcggcgg aagaaacaag gcgctgatac
    11941 ctgtcgatgg cgtcacggtc atctcgcgta ccatcagcct tctagagcct ctcttcagtg
    12001 agatgattgt tgcaggctgg cctcctgatg acccgctccc gggtgatgtc atacttgtgc
    12061 atgacaatta cgggggagtg gggcctctgg ccggcatcga ggcggcactg agggtggttc
    12121 gctcacccgt cctgttcgta ttcggtggcg acatgccatg gctctctgaa gagctcataa
    12181 gagcacaggc caatgagttt ctcaataatc ctccggatat tcttgttgcc aggtcaggcg
    12241 gacttgccga accgcttcac tccctttaca gccgtaaaat ccatcctgcc ctggtcggtc
    12301 atatcgaagg aggcggcagc aatgccatca ttgatttcta taaacttgct gacacacgat
    12361 attttgatct ccccctgacc cgggaggcac gtaaagccct gacgaacatc aacaggccgg
    12421 aagatttgct gatatgatcc tgattttttc ttatttttga tcaggacctt aagaaatcag
    12481 ccatgcgaaa ttctatcctt ctcatgatcg tggcagcagc gctccttagc tcatgccgga
    12541 aataccatga ttacagcaag acggaatgga cggaaaagga tttaccggaa tgggaagacc
    12601 tgacggtaaa taccataaat actgtcactc cccatgcctc aatggtgagc catcctgaca
    12661 actcttcagc actgaaggcg aagtggcgcg aatcggtcaa tgtgctgtcg ctcgacggga
    12721 agtggaaatt caactattct ccggcacctg ccgggcgccc ttattggttt ttcagggagg
    12781 attttgacac acgccagtgg ggtgagatag aggttccttc gacatgggag agggaagggc
    12841 acgggacagc ctattatgta aactcgggct acaccttccc ggtgaatccg cccttcatta
    12901 accattctga taacccggtg ggctcgtata agcgctcatt tacaatacca tccggctgga
    12961 aggagaagga ggtctttctc aactttgacg gtgtgagctc ggccttcaat gtctggatca
    13021 acggggaaaa ggtgggttac agcgaggaca gcaaaactac agccgaattc aatatcaccc
    13081 cgttcctcag gaagggtaaa aacaatattg ctgtagaagt ttaccgctac tctgacggca
    13141 gttacctgga gtgccaggat tttttcaggc tgagcggcat tcagcggtca gtctggttgc
    13201 atgccaggcc gaagacgtat atccgtgatt atttcgccgt accgacactg gtaaatgagt
    13261 acactgacgg tctcctgaac ctgaccgttg agctggccgg aaacgaaagg cggccttccg
    13321 agatgcgtct cgaggcaggg ctgtttgacg gggagacgaa gatattcgct gagaccaggg
    13381 atgtggaggc gtttgacagc ctggccgtag tggaactcac cgccgaagtg ccggatgtca
    13441 atccgtggtc tgccgagtca ccctcgctct atacgcttgt aattaccctt gctgacagcc
    13501 gcgggagggt gcttgagagc gtctcatcca ggataggttt ccgtacttcc gaagtgaagg
    13561 ggggacggtt cctgctaaac gggaaggcaa tttatctcaa ggggacaaat atgcacgagc
    13621 atgacccggt gcgcggtcac acaatagacg aggcgctgat gctgaaggac ataaggctga
    13681 tgaagatgaa caatatcaac gccgtgcgta cctctcacta cccgcagcct gaaagattct
    13741 atgaactgtg cgatgagtac ggactttacc tcattgacga ggcaaacatc gagtcgcacg
    13801 gcattggcta tcatcctgac attaccctgg ccaacaggcc tgagtggctc gggcagcata
    13861 tgtttcgtac cgtcagaatg gtggaacgcg ataagaatca cccttcagta atcatatggt
    13921 cattgggcaa tgaagccggt gacggccaga atttcgttga gacctacaaa tggatcaagg
    13981 gaagggatga aagcaggcct gttcagtatg aaagagctga gaaatcgacc cgcaccaatg
    14041 agaggcatac tgacatctgg tgcccgatgt atcctcccat agagagagtt gagtcatatg
    14101 ccctcagtga aggacaacca ggtttcgacc gtccgtatat aatgtgcgag tatgcccatt
    14161 caatgggtaa cagcactggt aacctgcagg attactggga tgtgattgaa aagtacggga
    14221 tactgcaggg agggtttata tgggactggg ttgaccaggg attgcttgag accagcgagg
    14281 agggggagga gtattttgcc tatggcggtg attacggcga ggtggggtca cccagtgacg
    14341 gcaacttctg ctgcaacggg ctggtggggc ctgaccgcac gccacacccc gcgctcaccg
    14401 aagtcaaaaa ggtctaccag tatgtgggat tcaacccggc ggaccttacc aggggactgg
    14461 tcaccatttc caataagtat gacttcacaa atctctcata tttcacaatc aactgggagg
    14521 tgctggccaa cggaatcctc ttccgctccg gcaaccttag acagatcagc ctggagccgg
    14581 gtgactcgct cactgtggag ataccctggg gggacattgc gccggcgccg gataccgagt
    14641 atcatcttaa cctgcatgtt tcgcgctccg atgagtgggg gattgtgccg caggatcaca
    14701 tgtacgcctc ggaacagatg cttttgccgt tccatgctgc ggcaaagact gctgcatcct
    14761 ctgtggcttc tgtggctgcc ggagcatcgg gggaagaaag ccttgcactc atcgatacgc
    14821 ggactgaggg atctgacctg acatttgccg gtgaaggttt cgaggtggtg tttgacatgg
    14881 ctgccggggt gatgaagtca tacagggttg caggccagga actgatcatg tcaggtcccc
    14941 gtcccgactt ctggagggcc cccattgaca atgactacgg caacggcatg gagaaaagaa
    15001 acgctccgtg gcgggaggca ggaggcaggg cagtgctgaa atcagcccgc atcagccagc
    15061 ccggcatggg cagcgcaggg gtggagttca ccttcgacat cccaggtgac ggagggaaga
    15121 agatagcagg tctggtgacg gggtatacgg ttcatgcggg aggttcggtt gaagtatcat
    15181 tcaacttaac caaaactgat atgagcctcg aagagattcc cagggtgggg atgcagatgc
    15241 tccttcccgg gaggttcgtt gacctctcct ggttcggccg ggggccgcat gaaaactatg
    15301 ctgaccggaa gacatccgcc ttcgccggag tatatgaaag ctcggtggct gatcagtatg
    15361 ttccttacgt aaggccacag gagaacggat acaaaaccga cacacggtgg ctgaatgtga
    15421 ccgacggaag cgggaaggga atccggtttg agggtgagcc catgttctcc tttgcagcga
    15481 tgaattacct gcatgaggat ttcgagtcgc agggcagcct ggcaggatac aggcccgatg
    15541 ccaaactggc taacagacat atcagtgatg tcattccgcg cgacctggta cgtgttaata
    15601 ttgactacgg acagatgggt gtgggaggtg ataactcatg gggtgcgcgc acacacccgc
    15661 agtactgcct cactgccagg gaataccggt acgggttcag aatggtaccg ctgaaatagt
    15721 ggcaccccgg cacagtcacc agtgccggtt tcagcctgaa acaactggtt ttttacttct
    15781 ttgccggcga tatttccacc ttcagcatgc tgttgcttcc ggtaacgata ttgtcgacat
    15841 tgtagacgag aggttgtgag aggtattttc tgtcagccct gaccaccacc attccgcgtg
    15901 caggcacggt aaacgttgcc ggcgctccct cggggccccc tgagagtttc atggggattt
    15961 ctgaagagtt gcagagctca aaccagatcc tggtgccgtc atccttaaaa ggtgctcctg
    16021 cagttatcac cgatctgaac agcggtgcag ctatctcttc aagtgctgcg agattatcac
    16081 cgtaccatac tgcagttctt cgtgcgaaca tcgcttcctt gagagattct ccggtacgtt
    16141 ctttagcaaa taccagtgtc atgggcctgt gagagtattc cggagcagcg tatccctcgc
    16201 tgatcacgtc atgaaaatcg ctgtttccca tgacagccag cttgttgtcg cgacacatat
    16261 ccatcaccaa cgggtagtac tcgacatgat tgtaatactc gattccgtgc agccacccct
    16321 tttcaatcag ttccctgtgc acatcgtaca tccgcggtat tccgtcaggc tgctggcttt
    16381 tccatccagg gtggttccac tggatgaatg cgccctgggc cactgcggct tcgattacct
    16441 tcatgaagtc atcctttacc agcgagtccg gttctgaaat aaacagtgcg ttgaggtggc
    16501 cgggaggcat cttgcgggtt atctccgaac ccttgacgag gatgatgtta taatcagctg
    16561 ccgttgcaga agctatcttc caggcagcgc tgtggtcaac aggtatgtat tgtttatgcg
    16621 gctgatattc aatatggtct gtgattgaaa tgacatcaag accatctcgc cacgcttcgc
    16681 cgacccggta tatgggccag acgtttccgt cggaaaagac agtgtgcatg tgaaagtcgc
    16741 atttcagggt cacatagccc ggcagttcgg gaacgctgag catgcgccgc tgggcctgca
    16801 gtgacatggt gcccagtaat aaaagcgata atgtaaagag tctgattttc atatatttgg
    16861 gatttgcgat ttgcgaattg cgattgattt tttcattgcc taatgcctaa t
//
LOCUS       DAOPXU010000058        16753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_3649_length_16753_cov_14.214217, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000058 DAOPXU010000000
VERSION     DAOPXU010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16753
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3649_length_16753_cov_14.214217"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..3278)
                     /locus_tag="PKK03_07320"
     CDS             complement(6..3278)
                     /locus_tag="PKK03_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNX84249.1"
                     /translation="MRKLKFTLMLLSLFIGTATMIGQVTTSTLSGKITESDGQSLPGA
                     TIIATHVPSGTVYGATANTQGLYSIQGMRPGGPYTVNVTFIGYTTQSYTEITLALGEN
                     TTLNSTLAPTTTEMTEVTVIGYATSRFNTTQTGAVTNITNNQIANMPSVSRSIIDVTR
                     LSPYGGNGMSFVGTDGRTANFTVDGANFNNNFGLSSALPGGGSPISIESIEELQVVIA
                     PYDVRQTNFIGGGVNAITKSGTNTFKGSLYSYHRNENMRGDAVNGRQIAGARDIDRNT
                     SYGFTFGGPIVKNKLFLFVNGEMAKTPTIVNRWRASTDGNADADNYISRTTVSDLKAV
                     SDYVKSKYGYDTGSYTDFPADENNYKLLARLDWNITDKHRLALRYNYTKNLYWSSPNA
                     SSMDGGTRMSEARMSQASMSFANSMYSMNNLVHSFSLDLNSRLSDNLSNQFLATFSKL
                     DDLRGSTSDEFPFIDILKDGQAYMSLGYELFTWNNAVHNNVWNIKDELTRYLGNHKII
                     GGIAYEYKMADNAYMRNGTGYYRYSSLADFLNEGTPEVVNLTYGYDGESNPAARIRTN
                     KIGVYGQDDWRVTGNFKLSYGLRIDGLFFDNNDLMTNQAILDLDYSGRHIDTGKWPTA
                     NLIVSPRIGFVWDTFGDKSLKVRGGTGLFAGNLPLVFFTNMPTNGGMVQYQAQINAAN
                     AAKKGFTMDEFAGGLVTDADGNASIAALYNKLTELGYPSTISPEDGTVPSAVNGVDPN
                     FKMPQVWKSSLAVDYTFKTSFPLSVTVEGIYNKTINGVSISDWSIPNVGGFARFNGVD
                     NRPIYPSGYRTNTKAFVLENTSLGYGWSGNITVNAQPAEWLNLMAAYAHTVAKDVTGM
                     PGSAAESAFTYVPTVEGPNYIRLHNSQYNTPDRLVGSLTARDNSGNHYSLIYEAWLGG
                     ANYSYMMLNDINSDGYNYDAIYVPTDEEVESNQFRFVSADDKTRFMDYVHANSYLKNQ
                     QGKYAEPYSVYSPWVHRIDFGYKHDFVLNGDNAKHTLQLSLDLKNILNMFNSEWGVAK
                     YLNPAIGSEARILKYEGVDADGVATFSTPSSINGDVKTFTPSYSLGQCWYALIGVKYI
                     FN"
     gene            complement(3441..4700)
                     /locus_tag="PKK03_07325"
     CDS             complement(3441..4700)
                     /locus_tag="PKK03_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNX84250.1"
                     /translation="MKTIYTGLIALYSAIAGAASPFSAKARRWVRGRRGWRERLSSFS
                     RGEGKVAWVHCASLGEFEQGRPVIEKIRRDHPDWKVVVTFFSPSGYEIRKDYRGADMV
                     MYLPTDLPANVRFFLDHIKPDLALFVKYEFWYNYLSELKRRNIPTYLVSGIFRPDQYF
                     FRWYGSFARGVFQVFNRIFLQDEQSGRLLESIGYHRYSVTGDTRFDRVSQIAAAAKDL
                     PVIEKFRGKESLFVAGSSWDEDEEIIVRYINSNSGAMKWVIAPHEIDEAHLCRIEKRL
                     TPESVRYSRYVEGAKSCRVMIIDNIGMLSSVYRYASIAAVGGGFGRGIHNILEPACWG
                     IPVLFGPHHLKFREAVQLKERGGAVSFDNFETFTSVVEKYLSDPVALEAAGNASAIYI
                     SENKGSTDKVYKEIFENQNAENLFKKR"
     gene            4766..5713
                     /locus_tag="PKK03_07330"
     CDS             4766..5713
                     /locus_tag="PKK03_07330"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNX84251.1"
                     /translation="MVRLVALFISVVLQIVAAGIAIRFMKITRYRLSWILLSTSFVLM
                     AVRTLIQLIEYFQGKPSFELMMVDEWMNVLISVMIITGVVLIREIFYSLKRAEIDRAR
                     SDRRLLNAVINTEETEKKRFAKDLHDGLGPLLSTVKMSLSALNSRVTDPVGQEILQNT
                     NHVVNEALNAIKEISNNMSPHVLTNLGVASAISAFAAKVNMMRGILIDFRTNMEGERF
                     DTDKEVVIYRAACEFINNGMKHSGASRIEIELRRDQKIITLQYLDNGRGFDMEKLSSE
                     ETVGMGIPNIETRVRSVDGVFVIESARGKGTSALIKVDQ"
     gene            5862..6503
                     /locus_tag="PKK03_07335"
     CDS             5862..6503
                     /locus_tag="PKK03_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX84252.1"
                     /translation="METIRIALADDHQLFRNGLKILLGGYPEFEVVAEASNGAELLKV
                     LENCPADIVLMDINMPEMDGVEATSRISRQIPAACVIALSMYGEEEYYYRMVDAGAKG
                     FILKDSDISDVHDAIIAVHRGGNYFSQELLYHVIRRIKNREHEEKSSSLSRREKEILL
                     KICEGLSNHEIADALFISKRTVDKHRANLLAKTGSKNTASLILYAIKNRLIEV"
     gene            6570..6932
                     /locus_tag="PKK03_07340"
     CDS             6570..6932
                     /locus_tag="PKK03_07340"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNX84253.1"
                     /translation="MSDRKGLGTIGEELAAAWLTGKGYSIRHRNWRSGRTEIDIIAEN
                     SSFIVFAEVKTRSAGYQVHPSEAVNVPKQRTIIFAASNYIDTYGITREARFDIITVII
                     GETGHEIDHIEGAYYPTM"
     gene            7076..8233
                     /locus_tag="PKK03_07345"
     CDS             7076..8233
                     /locus_tag="PKK03_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNX84254.1"
                     /translation="MPLNAQKSKKQAAPEPEGYKFTTVVSLPASPVKNQSATGTCWCF
                     ATTSFMESELLRMGKGEYDLSEMFVVRKTYENRILDNYLRQGKGNLGEGSLSPSWVRV
                     FNEDGIVPQEAYTGISYDSPTHNHRELQSYVDAVTAVPVKMRRLSKESNEVTDAILDI
                     YMGEVPATFTYKGQSYTPKTFAASLGLNMDDYVHITSFSHFPFYTEGLLEVPDNWEMN
                     RFHNVPLDELIQIMDYSLNNGYTVNWDGDVSERGFSHANGVAINPDLTRMDPSGNTDR
                     ARFEAGQGGQGANQAMFEKPGPEINVTQDVRQKGYETFVTSDDHLMHITGIVKDQNGT
                     KYYITKNSWGTDRNPQGGYLNMSESYVRAKTIFIMVHKNAIPADIKAKMGI"
     gene            complement(8236..9219)
                     /locus_tag="PKK03_07350"
     CDS             complement(8236..9219)
                     /locus_tag="PKK03_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNX84255.1"
                     /translation="MELPEEIFRISGAGSFEKLALEIFRFQADGCPVYGEYVSLLGIK
                     PEEVDSICSIPFMPISFFRDHLVMTGGGEPERIFLSSGTAGMKQSRHHVRDTAIYDES
                     LERTFRLFYGEPAEYAITGLLPSYLERDDSSLIYMANRLIQLSGNDAGGFFLDDHTAL
                     LQAIEKARAARLKVLLIGVTFALLDLAEKHPVDLSDVIIMETGGMKGRRREMIREEVH
                     EIIMKAFGVSSVHSEYGMTELLSQAYSRGGGLFRTPPWMKVLIRDSHDPMSHSSEAGA
                     AGGISVIDLANLWSCSFIATADLGRMHPGGLFEVQGRFDEADIRGCNLLVS"
     gene            complement(9219..9758)
                     /locus_tag="PKK03_07355"
     CDS             complement(9219..9758)
                     /locus_tag="PKK03_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84256.1"
                     /translation="MNRWFIPALALMLMFAGCGSDDQKTSGTIILSSDLYDGGSYYYA
                     MGLSFDEAGEVPTLPDQYRADIWLMAGQVTTGGPVVAFLTANTFTPPFALAGTYGTES
                     LAKNAFSELMTVGSYSWGEFASPLAAHQVWVVKTRDSKYAKLRIISVTLNTSVTPAAA
                     TCSFEWVWQPDGSSTFPAK"
     gene            complement(9755..11020)
                     /locus_tag="PKK03_07360"
     CDS             complement(9755..11020)
                     /locus_tag="PKK03_07360"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNX84257.1"
                     /translation="MKIIDNFNFKGLKAIVRVDFNVPLDKATREVTDDTRIRGALKTI
                     RKITADGGSAILMSHLGRPEGVMEKYSLKPVLPVLEKHLGMKVAFAPDALGEEAVAMA
                     AALRPGEVMMLENVRFYPEEEGKPVLPETATDEEKKAAKAALKVKQKEMAKKLSTYAD
                     VYVNDAFGTAHRAHATTAVIADFFPEDKRMFGFLINSELAAMDRVLKSAGKPFTAVMG
                     GAKVSDKILIIENLLDRVNNLIIGGGMTYTFIKATGGNIGNSLCEADKLDVAKAVIEK
                     AKAKGVSLLFPVDGINADKFDAEANTYTSAIDDVKDGWMGLDIGPKSIELFEKVIMDS
                     KTILWNGPMGVFEMAKFAAGTTAIAKAVAKATEKGAFSLIGGGDSVAAINKNGLADKV
                     SYVSTGGGAMLEYMEGKKLPGITAIRGEQ"
     gene            11255..11752
                     /gene="tpx"
                     /locus_tag="PKK03_07365"
     CDS             11255..11752
                     /gene="tpx"
                     /locus_tag="PKK03_07365"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNX84258.1"
                     /translation="MAKITLRGNPVNTSGSLPEKGSKAPGFTLVKSDLSTLSLNELAG
                     KKVILNISPSLDTGVCATAVRKFNQLAAGKENAVVLAITKDLPFAHGRFCSTEGITNV
                     VTLSGFRDAEFGKAYGVDLIDGPMAGLYARSIVVVDENGNVIYTELVPETVQEPDYDK
                     ALAAL"
     gene            11809..12936
                     /gene="holB"
                     /locus_tag="PKK03_07370"
     CDS             11809..12936
                     /gene="holB"
                     /locus_tag="PKK03_07370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNX84259.1"
                     /translation="MNFSQIPGQKAVIDRLRSSVAENRVSHALLFYGPGGSGKFAIAL
                     AFARYISCEKRTAEDACGICPSCVKYDKLIHPDLHFVFPVIKKKSGTESVSDTFIAQW
                     REMVLRSPWFSLSTWTEAMEVANEQALIPVAEAAEIIRKLSLKSFESDFKIMIIWLPE
                     KMNAETANKLLKMIEEPPARTLFILVSEEDDRLLPTITSRCQHIRIPSIQSDELSAHL
                     VKTMGMDHARASEIAAIANGNMVRAMELAREDDNSAVHLERFTKLMRTAWTRDIHSLV
                     TWSEETATLGRERQKSFLSYSLRMIRENFILNFNGRENRLVYLTRAEDEFSEKFHPFI
                     NEKNINALNREFNLAYAHVESNGNTKMIFLDLALRTMRLIR"
     gene            13121..14137
                     /gene="ricT"
                     /locus_tag="PKK03_07375"
                     /pseudo
     CDS             13121..14137
                     /gene="ricT"
                     /locus_tag="PKK03_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
     gene            14166..14654
                     /locus_tag="PKK03_07380"
     CDS             14166..14654
                     /locus_tag="PKK03_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HNX84260.1"
                     /translation="MRGSRSKIIAAALMIAVAAMACDRSIRYSENYSLDEGKWSMFDP
                     AKYTCAIDDTVKTYDIQLSLRTSTDYPYRNIYLFVVTSFPSGTFVTDTIQAMVTDEKG
                     RWLGKGTGDLRELTIPFKSNVYFPESGDYHFRVIHGMRDTLLNGVYDMGMKISLTGGK
                     AK"
     gene            14663..15361
                     /gene="trmB"
                     /locus_tag="PKK03_07385"
     CDS             14663..15361
                     /gene="trmB"
                     /locus_tag="PKK03_07385"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNX84261.1"
                     /translation="MGKNKHARWAEMKGFDRVIEPPFEEVFRHDHELKGKWHREWFGN
                     NNPIILELGCGRGEYTVGLAGLYPGKNIIGIDIKGARMWRGAKDAHLMGLPNSTFIRT
                     RIEFTSSFFAPGEIDEIWLTFPDPQMKRIREKKRLSGPHFLNLYRQYLKDRGIVHLKT
                     DCRELYDYTLALAKANGLGIITSVTDLHREMPGDPLLSIRTHYEELFLNQGVPITYLS
                     FRLPYEKEIISPGE"
     gene            15330..15686
                     /locus_tag="PKK03_07390"
     CDS             15330..15686
                     /locus_tag="PKK03_07390"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNX84262.1"
                     /translation="MKKRSSVPVSDNEGFFSRVYEVTKLVPYGRITSYGAIAKYLGTG
                     GSARMVGWALNSSHAGHGYVPAHRVVNRNGILTGKHHFGNSSTMQQLLENEGFTVEDD
                     RVVEFRERFWDPADEL"
     gene            15808..>16753
                     /locus_tag="PKK03_07395"
     CDS             15808..>16753
                     /locus_tag="PKK03_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNX84263.1"
                     /translation="MYKKEDILNALRKVTHPEKGTDIVTLGMVGDITQTGEGISLVLV
                     PEKSNDPFITSVKSNCVRAVKEALGPDAVIASIEVKPRIVVEKMAAPKRDVLPGVKNI
                     IAVSSGKGGVGKSTVAVNLAVALARKGYATGLLDADIFGPSVPRMFDAGEYHPDVRSE
                     NGTDIIVPMSKYGVKILSIGFFVRGSDAVIWRGPMASSFLKQLISQGDWGELDYLIFD
                     LPPGTSDIHLTLVQEVPVTGAIVVTTPQEVALADARKGIAMFRSDSINVPVLGLVENM
                     SYFEAPELPGRRYYIFGKEGGSRLAAESGTLLLGQIPIV"
BASE COUNT         4350 a         4162 c         4234 g         4007 t
ORIGIN      
        1 ttttattagt tgaaaatata tttgacgccg atcagggcat accagcactg acccaatgag
       61 tagcttggtg tgaatgtttt gacatcacca ttgattgaag agggtgtgga gaatgtagcc
      121 acaccatcag catccacacc ttcatatttg aggatacggg cttccgaacc gattgccgga
      181 ttcagatatt tggctacgcc ccactcagaa ttaaacatgt tcaggatgtt tttgagatcg
      241 aggctaagtt gcagcgtatg cttggcattg tctccattaa gaacaaaatc gtgtttgtag
      301 ccaaagtcta tgcgatgtac ccagggtgag taaacgctat agggctctgc atatttcccc
      361 tgttgatttt tcagataact gttggcatga acatagtcca taaagcgggt tttgtcgtct
      421 gccgaaacaa accggaattg attgctttcc acttcttcgt cggtgggaac atagattgca
      481 tcgtagttgt aaccgtcact gttgatatcg ttaagcatca tgtatgagta gtttgctccg
      541 ccaagccatg cctcatagat aaggctatag tgattaccgc tgttgtcgcg ggcagtaagc
      601 gaacccacca gacgatcggg tgtgttgtac tgtgaattat gcagtctgat gtagttgggg
      661 ccttccaccg tgggcacgta tgtaaatgca gattctgcag ccgatccggg cataccggta
      721 acatccttgg caaccgtgtg agcataggca gccatcaaat tgagccattc tgccggttga
      781 gcattgacag tgatgttgcc tgaccagcca taaccgaggc ttgtgttttc cagcacaaag
      841 gcttttgtgt tggtacgata accggaagga taaatcggac gattgtcaac accgttgaaa
      901 cgggcgaaac caccaacatt tggaatactc cagtcagaga tagaaacacc gttgattgtt
      961 ttgttgtaaa ttccttctac cgttacagac aatgggaaag aagttttgaa tgtataatca
     1021 acagcaagtg aacttttcca tacttgcggc attttgaaat ttggatctac accgttaaca
     1081 gcagaaggaa ctgtcccatc ttcgggagaa atcgttgaag gatatcccag ttctgttaac
     1141 ttgttataaa gtgcggcaat actggcattt ccgtcagcat cagttacaag accaccggca
     1201 aattcatcca ttgtgaaacc tttttttgct gcgttcgctg cattaatctg tgcctgatac
     1261 tgtaccattc caccgttggt aggcatattg gtgaagaata caagcggtaa attacccgcg
     1321 aaaagaccgg tacctccacg gactttcagg ctcttgtcgc caaatgtatc ccacacaaaa
     1381 cctatgcggg gagaaactat gagatttgca gtaggccatt tccctgtatc gatatgacga
     1441 ccggaataat caaggtccaa aatagcctgg tttgtcatca gatcattatt atcgaagaaa
     1501 agcccgtcga tacgaagtcc atacgagagt ttgaagtttc cggtaaccct ccaatcatct
     1561 tgcccgtata ctccaatttt gtttgttctt atgcgggcgg caggattgct ctctccgtca
     1621 taaccataag ttaagtttac aacttcaggt gttccttcgt ttaagaagtc ggcaaggctt
     1681 gagtagcgat agtatccagt accgttacgc atataggcat tatctgccat tttatattcg
     1741 taagctattc cacctatgat tttatgattg cccagatagc gtgtcagttc gtctttgatg
     1801 ttccagacgt tgttgtgaac agcattgttc catgtgaaaa gttcatatcc caaagacata
     1861 taggcttgtc cgtctttcaa aatatcgatg aaagggaatt catcggaagt agaaccgcgg
     1921 agatcgtcaa gtttcgagaa tgttgccaga aactggttgg ataagttatc tgacaatcgg
     1981 ctgtttaagt caagagagaa tgaatgaaca aggttgttca tagaatacat cgagtttgca
     2041 aacgacattg aggcctgtga cattcttgcc tcagacatac gggtacctcc gtccattgat
     2101 gaagcattcg gagagctcca gtaaaggttt tttgtgtagt tataacgcag ggccagacga
     2161 tgtttgtctg tgatattcca atccaaacgc gcaagaagtt tgtagttgtt ttcgtcagca
     2221 ggaaaatctg tatatgatcc tgtatcataa ccatacttac ttttaacata atcagaaacc
     2281 gctttaagat cgcttactgt tgtgcgggaa atataattgt cggcatcggc gttaccatcc
     2341 gttgaagctc tccaacgatt gacaatggtt ggtgttttag ccatttcacc gtttacaaat
     2401 aagaacaatt tgtttttgac aataggtccg ccaaaagtaa agccataaga tgtattgcgg
     2461 tcaatatcac gcgctcctgc gatttgcctg ccattaacag catcaccgcg catgttttca
     2521 tttctgtgat aggaatagag actgccttta aaggtattcg taccggattt tgtaatagcg
     2581 ttcacaccac cacctataaa gttagtctga cgaacatcat atggagcgat tacaacctgt
     2641 aattcttcga tagattcaat agaaatcggg ctgcctccac cgggcagggc agaactcaaa
     2701 ccgaagttgt tgttgaaatt cgctccatcc actgtaaagt tagccgtacg accatctgtg
     2761 cctacaaagc tcatcccatt ccctccgtaa ggtgagaggc gggtaacatc gataatactg
     2821 cggctgactg aaggcatatt agctatctgg ttatttgtga tattcgtcac ggcaccggtt
     2881 tgagtggtat tgaatcttga tgtagcgtaa ccaatcacgg taacctctgt catctcggtc
     2941 gtggttggtg ccagggtcga gttcagggtt gtattttctc cgagcgccag ggtaatctca
     3001 gtgtaactct gggttgtgta gccgatgaaa gttacattga ccgtataggg gcctccggga
     3061 cgcataccct ggattgaata caaaccctgg gtatttgcgg tggcaccata aacggtaccg
     3121 gaaggcacat gggttgcaat tatcgtggca cccggcaacg actgaccgtc ggactccgta
     3181 atcttgccac tgagagttga agttgtcacc tggccaatca tggtggctgt tccaatgaac
     3241 agggacaaca gcatgagagt aaattttagt tttcgcatag ttgagtaagt tatgaaagtt
     3301 atggttgtag tttaaatata ttaaatgtat tgaaagtcag ctggttcagg taaagcctca
     3361 gttgttcttg ggcagatggt taaattacag ttacgggcac aataagtcag aaatgacatt
     3421 tcggaattat taacaacttt ttaacgcttt ttgaataagt tttcagcatt ctggttttca
     3481 aatatttctt tatatacctt atctgtagat cctttatttt cagatatata gatagcactg
     3541 gcattcccgg ctgcctcaag cgccaccgga tctgagagat acttttcaac aactgatgta
     3601 aatgtctcaa aattgtcaaa cgagacggct ccccctctct cctttagctg cactgcctca
     3661 cggaacttca ggtggtgcgg gccgaagagc accggtatgc cccagcatgc aggctcgagt
     3721 atgttgtgta tcccccttcc gaaccctccc cccacggcgg caatggaagc ataacggtag
     3781 accgatgaca gcatgccgat attgtcaatg atcattaccc ggcaagattt ggctccctca
     3841 acgtatcgcg aatagcggac gctttcgggg gtaagcctct tctctatacg gcacaggtga
     3901 gcctcgtcta tctcatgcgg agcgatgacc catttcattg cgccgctgtt tgaatttatg
     3961 tacctgacaa ttatctcttc gtcttcatcc cagcttgagc ctgcaacgaa aagtgactcc
     4021 ttgccccgga acttttcaat taccggaagg tccttcgcgg cagcggcaat ctggcttacc
     4081 ctgtcaaaac gggtgtcacc ggtcacactg taacggtgat atcctatgct ttcaagaaga
     4141 cggcctgact gctcatcctg gagaaaaatg cggttgaaca cctgaaacac acctcttgcg
     4201 aagctgccgt accacctgaa gaagtactgg tcaggcctga agattccgga gacgagatag
     4261 gtcgggatat ttctcctctt gagctcgctg aggtaattgt accagaactc gtacttgaca
     4321 aacagcgcaa gatcaggttt tatatggtca agaaaaaacc tgacattggc aggcagatcc
     4381 gtcgggaggt acatgaccat gtcagctcct ctgtaatcct tcctgatttc atacccggag
     4441 ggagagaaaa aggttacaac cactttccag tccggatggt ccctccttat cttttcaata
     4501 acaggcctgc cctgttcaaa ctcaccgaga gatgcacaat ggacccaggc cactttccct
     4561 tctcccctgc tgaatgaact gagccgttcc ctccatccgc gcctgcctct cacccaccgg
     4621 cgggctttgg cgctgaaagg cgacgctgct cctgcaatgg cagagtaaag tgcgatgagg
     4681 ccggtataga tggttttcat ttcccgattt tggcgcccca aaggtaataa actcatactt
     4741 ttgtatgaat taaatttttg ttgcaatggt acgcctggta gccctgttca tatcggttgt
     4801 gctgcagata gtggctgcag gcatagccat aagattcatg aagataacac gttaccgtct
     4861 ctcatggatc cttctttcca catcgttcgt actgatggca gtacgcactc tcatccagct
     4921 catagagtat tttcagggca aaccctcgtt cgagctgatg atggtggatg agtggatgaa
     4981 cgtgctgatc tcggtgatga taattacggg cgtggtgctg atcagggaga tcttttattc
     5041 actgaaacgt gcggaaattg acagggcaag atctgacagg aggctcctga acgctgtaat
     5101 caatacagag gagactgaaa aaaagcggtt cgcaaaggat ctgcacgacg gactgggtcc
     5161 gctgctctct accgtaaaga tgtctctctc agccctgaac agcagggtca ccgacccggt
     5221 gggacaggag attcttcaga atacgaacca tgtcgtaaac gaagctctga atgccatcaa
     5281 ggagatctcc aacaacatga gtccacatgt gctgaccaac ctgggggtgg caagcgccat
     5341 cagcgccttc gcggcaaagg tgaacatgat gcggggaata ctgatagact tccggacaaa
     5401 catggagggg gagcggtttg acactgacaa ggaggtggtt atataccgtg cggcatgcga
     5461 gtttatcaac aacggcatga agcactccgg tgcttccaga atagaaatag agcttcgccg
     5521 cgaccaaaaa ataattacct tgcagtatct tgacaacggc cgcggattcg acatggagaa
     5581 gctgtcatcg gaagagacgg tgggaatggg aattccgaac atcgaaaccc gcgtgagatc
     5641 agtcgacggg gtctttgtca ttgaaagcgc acgcggcaag ggaaccagcg cgctgattaa
     5701 ggtggaccag tgagatttgc gaatggcgat ttgcgatgtt cgattttgtc cgagagacat
     5761 aaagcattgt agaagaataa catatgacat gccaacaggg catcaggcat tgcagcaaaa
     5821 tgcataaaca aaaccaatcc ccgtagggga taaagcattg tatggaaaca atcagaatag
     5881 cacttgcaga tgaccaccag ctcttccgga acggactgaa aatcctcctc ggcggctatc
     5941 ccgaattcga ggtggtggct gaggcatcaa acggcgccga actgctgaag gtgctcgaaa
     6001 actgccccgc tgatattgtc ctcatggaca tcaacatgcc tgagatggac ggggtggaag
     6061 ccacatcgcg tatttcccgc cagatcccgg ccgcatgcgt gattgcactg tcaatgtacg
     6121 gtgaggagga atattattac aggatggttg atgccggagc gaaggggttc atactgaagg
     6181 attccgacat ctcggacgtg catgacgcaa ttatcgcggt gcaccgcgga ggcaactact
     6241 tctcgcagga gctgctttat catgtcatca ggcgcatcaa gaacagggaa cacgaggaga
     6301 agagctcaag cctctccagg cgcgagaagg agatcctcct caagatctgc gaaggactct
     6361 ccaaccacga gattgccgat gcactgttca tcagcaagcg tacggtagac aagcaccggg
     6421 ccaatctgct tgccaaaacc ggttccaaaa acaccgccag cctgatcctg tacgctataa
     6481 agaacagact gattgaagtt tgatttctcc aaaaaggaat caggtagcgg ccttgataat
     6541 ccgtcttccc ccgccagtgc aagtatgata tgagtgaccg caaaggactg ggaaccatag
     6601 gtgaagaatt agccgctgcc tggctgacag ggaagggcta ttcaatccgc caccggaact
     6661 ggcgctcggg acgcacggaa atagacatta tagctgaaaa cagcagtttc attgtattcg
     6721 ccgaagtaaa gacaagatct gcaggatatc aggttcaccc ttccgaggct gtcaacgtgc
     6781 ccaaacagag gacaatcatc ttcgcggctt ccaactacat cgatacttac ggtatcacca
     6841 gagaggccag gtttgatatc ataactgtaa taatcgggga gactggccat gagattgacc
     6901 acattgaggg cgcctactat ccgaccatgt gattttccgc ccacggcgct gctgcttaaa
     6961 aacatttccc ttttaaatat tattgtatat ttttgattcg tcctgaattg ataaaccaaa
     7021 aaagatacaa atgaaaaaga cagtattaat cctcatggca atggctatcg ccgccatgcc
     7081 gctgaatgcc cagaagagca agaaacaggc cgctcccgag cccgagggat ataaattcac
     7141 caccgttgtc tcccttccgg cctcgccggt taagaaccag tcggccacag gcacatgctg
     7201 gtgtttcgca accacttcat tcatggagtc ggagctcctg aggatgggca agggtgaata
     7261 tgacctctcg gaaatgttcg tggttagaaa aacatacgag aacaggatac ttgacaatta
     7321 tcttcgccag ggaaaaggaa acctgggtga gggcagcctt tcgccttcat gggtaagggt
     7381 tttcaacgag gacggaatag ttccccagga agcatacaca ggcatcagtt atgattcccc
     7441 gacccataat caccgtgagc tccagtctta cgtcgatgcg gtaactgctg ttccggtaaa
     7501 gatgagacgc ctcagcaagg agtcaaacga agtaaccgac gctattcttg acatttacat
     7561 gggagaagta cctgccacct ttacctacaa gggtcagagc tatactccga aaacatttgc
     7621 agccagcctg ggactgaaca tggatgatta tgttcatatc acctccttct cacacttccc
     7681 cttctatacc gaaggacttc ttgaagtgcc tgacaactgg gagatgaacc ggttccacaa
     7741 cgtgcccctc gacgaactga tacagatcat ggactactca cttaacaacg gttatacggt
     7801 taactgggac ggtgatgtaa gcgagagggg cttctcccat gccaacggcg tagccatcaa
     7861 tcccgatctc accaggatgg atccgtcagg caacactgac agggcccgtt tcgaggcagg
     7921 tcagggtggc cagggcgcca accaggcaat gtttgaaaaa ccgggtcccg aaattaatgt
     7981 aacccaggat gtccgtcaga agggatacga aacattcgta acctccgatg accacctgat
     8041 gcacatcacc ggtattgtaa aggaccagaa cggtacgaag tattacatca cgaagaactc
     8101 atggggcaca gacaggaatc cacagggtgg ttacctgaac atgtctgaga gctatgtgag
     8161 ggccaagacg atcttcatca tggttcataa gaacgccatc cctgcagata tcaaggccaa
     8221 aatgggaatc tgacttcagc ttaccagcag attgcatccc ctgatatcag cctcgtcaaa
     8281 ccggccctga acctcaaaga gcccgccggg gtgcatccgg ccaaggtcgg cagttgctat
     8341 gaatgaacat gaccagaggt tggcaaggtc aatgacactt atgccgccag ccgctccggc
     8401 ctctgaagaa tgtgacatcg gatcatggct gtcacgtatc agcactttca tccagggggg
     8461 ggtacggaac agtcctcctc cccgggaata agcctggctg agcagttcgg tcatcccgta
     8521 ttctgagtgt accgagctga caccgaaggc tttcatgatt atctcatgca cctcctccct
     8581 gatcatttca cgcctccggc ctttcatgcc gccggtctcc ataatgatga catcgctgag
     8641 atcaaccgga tgcttctccg ccaggtcaag cagcgcaaaa gtgactccta tcagcagcac
     8701 cttcagtcgt gcggctctgg ccttttcaat agcctgcaga agagctgtat ggtcatccag
     8761 gaaaaaaccg ccggcatcat tgccagagag ttgaatcagc ctgttagcca tataaatgag
     8821 agatgagtca tctctctcaa gatacgaggg aagcagcccc gtgatggcat attccgcggg
     8881 ttcaccgtaa aaaagcctga atgtccgttc caggctctcg tcatagatgg ccgtatccct
     8941 gacatggtgc cggctctgtt tcatgccggc cgtgccgctg ctgagaaaaa tccgttcagg
     9001 ttcccctccg ccggtcatga ccaggtgatc cctgaagaac gagatgggca tgaaggggat
     9061 tgaacagatt gaatcaacct cttccggttt aatcccgaga aggctgacat attcaccgta
     9121 gacggggcat ccgtcagcct gaaagcgaaa gatctccagt gccagctttt caaatgagcc
     9181 ggcgccggag atcctgaata tctcttccgg gagctccatc acttcgccgg gaaagttgac
     9241 gatccgtcag gctgccacac ccactcaaag gagcaggtgg cggctgcggg agtgaccgaa
     9301 gtattcaatg tcacgctgat tatcctgagc ttggcgtatt tcgagtcgcg tgtctttaca
     9361 acccatacct ggtgggctgc aagcggtgat gcaaactcac cccacgaata gcttcccacg
     9421 gtcatcagtt cgctgaaggc attcttcgcc aggctttcgg ttccgtatgt tcccgccagg
     9481 gcgaatgggg gagtgaatgt attggcagta aggaatgcaa ccaccggtcc gccggttgtc
     9541 acctgccctg ccatcagcca aatgtcagcc cggtactgat cgggaagcgt cgggacctcc
     9601 cctgcctcat caaaggagag tcccatcgcg taataataag acccgccgtc gtacaggtca
     9661 cttgacagaa tgatggttcc ggaggtcttc tggtcgtcgc ttccgcaccc tgcaaacatc
     9721 agcatcaggg ccagcgccgg tatgaaccaa cggttcactg ttcacccctg attgctgtga
     9781 tgcccggcag ctttttgcct tccatgtatt ccagcatggc gcctccgccg gtggagacat
     9841 agctcacctt gtcggccagt ccgttcttgt tgattgctgc aaccgagtcg cccccgccaa
     9901 tcagtgagaa tgctcccttt tcggtggcct ttgccacggc ctttgctatt gcggtggttc
     9961 cggcggcaaa ctttgccatc tcaaacacac ccatcgggcc gttccagagg atggtctttg
    10021 aatccataat caccttttca aaaagttcta tgctcttcgg tccgatgtca agacccatcc
    10081 atccgtcctt aacatcatca attgcagaag tatatgtatt agcttcagca tcaaacttgt
    10141 cagcattgat cccgtcaacc gggaagagca ggctgacccc ctttgccttt gccttttcga
    10201 tgaccgcctt tgccacatca agcttgtcgg cctcgcacag ggaattgcct atgtttccgc
    10261 ccgtagcctt tatgaaggta tatgtcatgc caccgcctat aatcaggttg ttcaccctgt
    10321 caagcaggtt ctcgatgatc agtatcttgt ctgacacctt ggctccaccc atgaccgcgg
    10381 tgaatggttt cccggcgctc ttcagcaccc tgtccattgc agcaagttcg ctgttgatga
    10441 ggaatccgaa catccttttg tcttccggaa aaaagtcagc tattaccgcg gtggtggcgt
    10501 gagcccggtg agccgtgccg aatgcgtcgt tgacatatac gtcggcatag gtcgaaagct
    10561 tcttagccat ctccttctgt ttcaccttca gagctgcctt ggcagctttc ttttcctcat
    10621 cagtggcggt ctccgggagt accggcttgc cttcctcctc gggatagaac ctgacatttt
    10681 cgagcatcat cacttcgccc ggtctgaggg cggcggccat cgccacagcc tcttcgccaa
    10741 gggcgtcagg agcaaaagca accttcatcc cgaggtgttt ctccagcacc gggagaacgg
    10801 gtttgagtga atatttctcc attactcctt ccggccgccc caggtgtgac atgagaatgg
    10861 cacttccgcc gtcagcggta atcttcctta tcgtcttaag cgctccgcga atgcgggtgt
    10921 catcagtaac ctcgcgcgtg gctttgtcga ggggaacatt gaaatcaacc cggacaattg
    10981 ccttgagacc cttaaaatta aaattgtcaa tgattttcat aaaatttctg tttacggatt
    11041 acctgtttgt ttgtattgta caaaaataag aagttgaaag gttcccttac aagcaaaggg
    11101 gtaattatgt cagtttattt tttattaaac aaatgccgtg gtgatttgtt aaatttgaga
    11161 aaactaaggc agaacatgcg cccggcagaa caggttgcat ggaatttcac tgaaagatta
    11221 cgcaaagcga agtattaaaa aaaataaatg aattatggca aaaattactc tcaggggcaa
    11281 ccctgtaaac acatcaggtt cgcttcccga aaagggaagc aaggctccgg gcttcaccct
    11341 ggtaaagtca gatctcagca ccctttcgct gaatgagctg gcaggaaaga aagttatact
    11401 taacatttct ccaagtcttg atacgggcgt ctgcgccacc gccgtacgca aattcaatca
    11461 gctggcagcc ggaaaggaga acgctgttgt gctggcaatc acgaaggatt tgccgttcgc
    11521 ccacggacgt ttctgctcaa ctgaagggat aacaaatgtg gttacactgt cagggttccg
    11581 tgatgccgaa ttcgggaagg cttatggcgt tgacctcatt gacggaccga tggccggtct
    11641 atatgcccgc agcattgtgg ttgttgacga gaacggtaat gtgatttaca ccgagctggt
    11701 gccggagaca gtccaggagc ccgattacga caaagccctg gcagcgcttt aaaaaacatt
    11761 actaatccgt tttttactac atttgctctt cgtcatccga agggcaaaat gaatttttca
    11821 cagataccgg gacagaaggc agtgattgac aggctgcgga gctccgttgc ggagaaccgc
    11881 gtcagtcatg cactgctttt ttatggtccc gggggaagcg gcaagtttgc catagcgctg
    11941 gcctttgcaa ggtacatcag ctgcgagaaa cgcaccgcgg aagatgcctg cggcatttgc
    12001 ccgtcttgcg tcaagtatga caagctgata catcctgacc tccactttgt cttcccggtc
    12061 attaaaaaga aatcgggcac tgaatctgtc agtgatacct ttattgcgca atggcgcgaa
    12121 atggtactcc ggtcaccgtg gttctcgctc agcacatgga cagaagctat ggaggtcgcc
    12181 aacgagcagg ccctgatacc tgtggccgag gcggcagaga ttatccggaa gctcagcctc
    12241 aaatcatttg aatctgactt caagataatg atcatctggc ttcccgaaaa gatgaatgcc
    12301 gagacggcaa acaagctcct caagatgatt gaggagcccc ctgcccgcac cctcttcata
    12361 cttgtttccg aagaggatga caggctgctt ccgacgatca cctcgcgatg tcagcatata
    12421 aggattcctt caatacagtc agatgaactc agcgcacacc tggtcaaaac catgggcatg
    12481 gaccatgcca gggcctcgga gatagccgct atcgcgaacg gcaacatggt cagggctatg
    12541 gaacttgccc gggaagatga caacagcgcg gtgcacctcg agcggttcac gaagctgatg
    12601 cgcacggcgt ggacccgtga catccactcc cttgtgacat ggtcagagga gaccgcaacg
    12661 ctgggaaggg aaagacagaa gagcttcctc tcctactcgc tgaggatgat acgtgaaaat
    12721 ttcatactca atttcaacgg gagggaaaac agacttgtat acctcacccg cgccgaggat
    12781 gagttttctg aaaaattcca tcccttcatc aacgagaaga acatcaatgc cctcaaccgt
    12841 gagttcaacc ttgcatacgc ccacgtcgag agcaacggca ataccaaaat gattttcctt
    12901 gacctggccc tcaggaccat gaggcttatc aggtaaacga ggggaatcac ttgataatag
    12961 ctcttttatt cctaattttg cattcaccct tgcggaaggc atgaaactga ccctttcttc
    13021 cgccagcggt taacatgatg ttatgataaa cgaggatata caggtccagc ccggcgagga
    13081 gccgccaaag cctgaaataa aatacacaat cgggacacac aagcttaatg agtacaactg
    13141 gcttgccgac ctccccttcg acatacgtca caatgatgtt gttgaagtcc ggttcaagaa
    13201 caccagaaag ggtttctacc gcaatgttaa cggcctgaaa ctggaagttg atgacattgt
    13261 tgcggtggag gcctctcccg gacatgatat cggttatgtg tcaatgaccg gcttccttgt
    13321 cagtcagcag cttaagaaaa tgaagacaag gcctgtagtc gatgagctga agaaagtcta
    13381 ccgtaaggcc cggcctgttg accttcagaa atgggaggag gcaaagaagc ttgaggttac
    13441 caccatgctt cgctcgagga agattgcaga aaacctgggg ctggcaatga agatcggcga
    13501 tgttgagtac cagggtgaca agaccaaggc catcttctac taccttgccg atgagagggt
    13561 tgatttccgc cagctgatca aggtccttgc cgaggagttt catatcagga tagagatgag
    13621 gcagataggt gcgcggcagg aggcagggcg tataggaggc attggcacct gcggcaggga
    13681 gctgtgctgc tctacccatg tgacaaattt catttcggtg actacttccc acgcacgcta
    13741 ccaggatctg tccctcaacc cgcagaagct cgccgggcag tgcggcaaac tgaagtgctg
    13801 cctgaactat gagcttgact gctacattga tgcgcagaag gaattcccct cgaaggagat
    13861 accccttgag cttgccggag gcacagccta cttcatgaag atggaggtgc acaccggcgt
    13921 atactggtat tcgactgatc cgcggattgc cgctaacatg actgcagtgc ctgtggcacg
    13981 ggtacgtgag atcatccagc tgaaccgtaa aggagtcaag cctgataacc tgctggccgg
    14041 accggcgaac aaccgcatgg aaagaggcaa tgacctgctt ctgcagaaca gcgggcttga
    14101 caggtttgaa tcaaagagcc gcccgcgtga aggcaaaggc aaaaacagac catatcacag
    14161 gagaaatgag aggaagcaga agtaagatca tagcagccgc actgatgatc gcagtggctg
    14221 ccatggcatg cgaccggagc atacggtact cggagaacta cagcctggat gaaggcaaat
    14281 ggagcatgtt tgatccggca aaatacacct gcgccattga tgacactgta aagacatacg
    14341 acatacagct gtcattgcgc acctcaacgg attacccgta ccgcaacatc tacctctttg
    14401 ttgttacatc cttcccttca ggcacttttg taactgacac cattcaggcc atggtgaccg
    14461 atgaaaaagg caggtggctg ggcaagggaa cgggtgatct gcgtgaactg accatcccct
    14521 tcaagtcaaa tgtatatttc cccgagtcag gcgactatca cttcagggtt atccacggta
    14581 tgagggacac cctccttaac ggcgtctatg acatggggat gaagatttcg ctgacaggcg
    14641 ggaaagcaaa ataacctggg ttttgggcaa gaacaaacac gcccggtggg ctgagatgaa
    14701 agggttcgac cgtgttatcg agcccccttt tgaggaggta ttcaggcatg accacgagct
    14761 taagggaaag tggcacaggg aatggttcgg caacaacaat cccattatcc tggaactcgg
    14821 atgcggcagg ggagaataca ctgtggggct tgcaggcctg tatcccggga agaatataat
    14881 cggcattgac atcaaggggg cgcgcatgtg gcggggcgcc aaggatgcac atctcatggg
    14941 gcttccgaat tccacattca tccgtacgcg tatagagttc acaagctcgt tcttcgcccc
    15001 cggcgaaata gatgagatat ggctgacctt ccctgatccc cagatgaaga ggataaggga
    15061 aaagaagagg ctctcaggcc cgcatttcct gaatctttac aggcagtacc tgaaagacag
    15121 gggcatagtg cacctgaaga ccgactgccg cgaactgtat gactatacac tggcgcttgc
    15181 gaaggcaaac ggcctcggga taatcacatc cgtcactgac cttcaccggg aaatgccggg
    15241 tgatccgctc ctctcaatca ggacacatta cgaagaactt tttctgaatc agggtgttcc
    15301 gataacatat ctgtcgttca gactgccata tgaaaaagag atcatcagtc ccggtgagtg
    15361 acaacgaagg cttcttcagc cgtgtgtacg aggtaacgaa actggtaccc tatggccgga
    15421 ttacctcata tggcgccatt gcgaagtatc tcggcaccgg aggctccgcg agaatggtgg
    15481 gatgggccct caattcatcg cacgccggtc acgggtacgt cccggcccat cgggtggtaa
    15541 accgcaacgg tatactcaca gggaaacatc acttcggtaa ctcgtcaacg atgcagcaac
    15601 tattagagaa tgagggtttt acggttgaag acgacagggt ggttgaattc agggaacggt
    15661 tctgggatcc ggccgatgaa ctatagatgg aagattgtgt tggtaaacaa gaaggtctga
    15721 aggtctgaag gtctgaaggt ctgaaggtct gaaggtctga aggtcttgaa aaaaatcgca
    15781 aatcgcaaat cgcaaataat caatatcatg tacaaaaaag aagatattct gaacgcgctt
    15841 cgcaaggtca cccacccgga gaagggtact gacatcgtca ccctcggaat ggtgggagac
    15901 attacccaga ccggcgaggg aatctccctg gtgctcgtac cggaaaaatc aaatgaccct
    15961 ttcattacat ccgtcaagag caactgcgtg agggctgtaa aggaagccct cggccctgat
    16021 gcggtcatcg catcgataga ggtcaaaccg aggatcgtgg ttgaaaaaat ggcagcgccg
    16081 aaaagagatg tgctgccggg cgtgaagaac atcattgcag tttcctcggg caagggaggc
    16141 gttggcaagt caaccgtggc cgtgaacctg gcagtggcgc tggcacgcaa gggctacgcc
    16201 accggcctgt tagacgccga catcttcggc ccctcggtgc cgagaatgtt cgatgccggt
    16261 gaataccatc ccgatgtacg cagtgagaac ggaacagaca tcattgtccc gatgtcaaag
    16321 tatggcgtga aaatactctc aataggtttc ttcgtcaggg gctctgacgc cgtcatctgg
    16381 cgaggaccca tggccagcag ttttctgaag cagcttatca gccagggcga ctggggtgag
    16441 cttgactatc tcatcttcga cctgcctccg ggtacaagtg acatacacct gacgcttgtt
    16501 caggaggttc cggttacagg agccattgtg gtgaccacac cgcaggaggt ggctctggca
    16561 gatgcgcgga aagggattgc aatgttccgc agcgactcca tcaacgtgcc ggttctgggt
    16621 ctcgttgaaa acatgtcata cttcgaagct cccgagcttc ccggcaggag atactacatt
    16681 ttcggcaagg aaggcggaag caggcttgcc gccgagagcg gcaccttgct tctggggcag
    16741 atacccatcg tgg
//
LOCUS       DAOPXU010000059        16686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_3665_length_16686_cov_12.064518, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000059 DAOPXU010000000
VERSION     DAOPXU010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16686
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3665_length_16686_cov_12.064518"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..2225)
                     /locus_tag="PKK03_07400"
     CDS             complement(81..2225)
                     /locus_tag="PKK03_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNX84264.1"
                     /translation="MDTSVHIRTIARKFQLHDWQVENTLRLLDDGATIPFISRYRKEV
                     TGSLNEVQLAEIRDEYERLKELEKRREAVIRSIEEQEKMTPELLAKINAALTMSQLED
                     LYLPFRPKRKTRASVAREKGLEPLALWLLAQNTGDTEAEAGKYVGDNVEDTTAALSGA
                     RDIIAEIASENEQVRGSLRRLYERDAVITSKVVKGKEQEGAKFSDYFDWSEPLRRCPS
                     HRLLAMRRGEEEGFLRITIMPSEEEAQSLVEKQFVKANNDSARQVREAVADSWSRLLA
                     PSMETEFRKTSKEKADEEAIRVFTENLRQLLLAPPLGEKNVLGIDPGQRTGCKLVCLD
                     RQGALLHHDVIYPHPPQNETARSVSKMLSLVDKFSIEAVAIGNGTASRETEQFVRSYL
                     PAESGIKVFVVSEAGASIYSASKTARDEFPDHDVTVRGAVSIGRRLMDPLAELVKIDP
                     KSIGVGQYQHDVDQTRLQASLDEVVMSCVNAVGVEVNTASPHLLTYVSGIGPKMAQNI
                     VDHRTENGPFRSRTELMKVKRMGEKAFEQAAGFLRIRGAANPLDASAVHPESYHIVGK
                     MAADLGCSVTALIKDEARRKSIMIEKYVTAEAGLPTLKDIMQELARPGRDPRQAIQEF
                     SFADIHSIDDVKEGMVVPGIVTNITRFGAFVDIGVKQDGLVHVSQMADRYVDDPAKIV
                     KLHQHVTVRVVSVDRNRNRIQLSMKGEKVRRQ"
     gene            complement(2232..3416)
                     /locus_tag="PKK03_07405"
     CDS             complement(2232..3416)
                     /locus_tag="PKK03_07405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNX84265.1"
                     /translation="MPANIILKKGKEQSVRRYHPWIFSGAVASVNGNPSEGDVVDVFA
                     WDKTYLATGHWAPGSIAVRIFSFTKCNPDHGFFRKRLEEAMAFRINAGIAGKPETTVW
                     RLVHGEGDGLPGLIVDIYGSIAVMQAHTAGFWKIREMIAQLLPEVTGGLVTAVYDKSE
                     GTLPFMARLNPSNGYLVGENGEPGAVVTENGYRFRVDWEKGQKTGFFIDQRDSRSLLS
                     RYSNGRRVLNMFGYTGGFSVYAMKNAELVHTVDSSAAATALADENVKLNFGDDPRHHC
                     YTTDAFTYLAGMEREYDLIILDPPAFAKHQTALNQALQGYRRLNALAIHKIRPGGLLF
                     TFSCSQVVSRDDFRRSVFVAAANTGRNVRILHQTGQPADHPVNIYHPESEYLKGLILY
                     IE"
     gene            complement(3427..4020)
                     /locus_tag="PKK03_07410"
     CDS             complement(3427..4020)
                     /locus_tag="PKK03_07410"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNX84266.1"
                     /translation="MTKQYNGSITDDELRRLPLLQFEGRVTLVDNMEKFRRVMGDIGR
                     PSLLGFDTETRPSFKKGRRYKVALLQLADADRAWLFRLNMIGLPPELAALLSDRNIVK
                     TGVAIRDDIKALRALTPFEPHGFIDLQSVVVDHGIQELGLKKLTAIVLGYSISKSQQV
                     SNWEAPALTEPQQLYAATDAWVCRSIYLALNGKETYQ"
     gene            complement(4004..4564)
                     /locus_tag="PKK03_07415"
     CDS             complement(4004..4564)
                     /locus_tag="PKK03_07415"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HNX84267.1"
                     /translation="MEKEEKVIYSRQVVEFAASANEYCKYLESTREINGIEILKVLQR
                     LLPFIYLRGSLLPLLEPVLEEPNEKTVTEFDWTRMHENLLSKLGNNDPFPVIVSVGDP
                     ADGLYTGSISENLSDIYQDLKDFIVSYKSGNEEVMNDAVWEVLMNFEEFWGKKLLNVL
                     TAIHTVLYSEDDTIAGNEQNEDDETI"
     gene            complement(4639..6549)
                     /locus_tag="PKK03_07420"
     CDS             complement(4639..6549)
                     /locus_tag="PKK03_07420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HNX84268.1"
                     /translation="MLPDIYRETLARYWGFTSFRPMQEEIIESVVSGSDVVGLLPTGG
                     GKSVTYQLPPVAGEGLCLVITPLIALMKDQVVRLKTMNIKAMAIHSGMTREEVEISLD
                     NCLYGDYRLLYVSPERLQTPMFRARLPRFNFTLVAVDEAHCISQWGYDFRPSYLHIAE
                     IRDMMNEDVPFLALTATATPRVVDDIISRLKMKNPKVHRTSFRRPNITYMVREVEDKN
                     TYVLRSLKKEQGSGIIYVSSRKRAKEVAEMLVANNIKADFYHAGLPQEMRDRKQTSWS
                     AGETRVIVATNAFGMGIDKADVRFVIHWDCPDSVEAYYQESGRAGRDGKPSFSVLLWS
                     QDEKRKLADSVRIKFPPVERIKDVYEAIGNFYQLPVGSGRNSVHDFDLWKFVSAFRFS
                     VTETYNSLNFLQKEGYLEFTDEINNPSRVHFVVTRDDLYKFQVANEEYDRFIKLLLRT
                     YSGMFSEFVPVNEENLAARSGLTREAIYQYLLRLSSQGIIHFIPGKKSPLVIFTEERL
                     DRSHLRISPELYLKVKENYIERVARITDYAENKTRCRPAFLAAYFGEESGRCGLCDTC
                     QERNELELSKYEFDLIIDKIKELLAEKPLRPEELEEQIDLPREKSIKVIRWLLDHEKL
                     VYDSDHRLTWKR"
     gene            6680..8170
                     /gene="guaB"
                     /locus_tag="PKK03_07425"
     CDS             6680..8170
                     /gene="guaB"
                     /locus_tag="PKK03_07425"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNX84269.1"
                     /translation="MSFSEDKLLFEGLTFDDVLLLPAYSEVLPRNVDLRSKFTRNIII
                     NTPIVSAAMDTVTEAELAIAIAREGGIGVIHKNMSVSQQAAQVRKVKRAESVMIFDPV
                     TISDGASVADALALMKEFKIGGIPVIHEDGALAGIVTNRDLRFETDYSRRISEIMTPL
                     ERLITLSHQTDLAAAAEILQKHKIEKLPIVDEKGRLKGLVTYKDITKAKDRPNACKDD
                     KGRLRAAAAVGIAPNTLSRVEALVGAGVDAIAIDTAHGHTKSVIEILREIKKNFPRTE
                     VVVGNIGTAEAARKLVEEGADAVKVGIGPGSICTTRVVAGVGVPQLSAIYNVARAIEG
                     SGVPVIADGGIRYSGDIVKAIAAGADSIMAGSLFAGVEESPGETIIYNGRKFKTYRGM
                     GSVEAMQMGSKDRYFQDMEDDIHKLVPEGITARVPYKGTLTEVIYQFLGGLRSGMGYC
                     GARDIATLKREGRFVRVTTAGVTESHPHDVTITREAPNYSRKINEY"
     gene            8173..9021
                     /locus_tag="PKK03_07430"
     CDS             8173..9021
                     /locus_tag="PKK03_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84270.1"
                     /translation="MVRLIIPLLLLAVVSCRHAGTDENRNAVARAGDRVLYLDQIPSG
                     LMTEGMSKADSTSAVQSYIRQWARKELMALRAEENLTPEYKAEVNRQLNEMRNNLLIY
                     QYQHQMIIQRMDTTVTDNEVQDYYVSNLSTFTLTSNLIRALYIKVPEGMSELDRIRKL
                     YRSQESADIRELEDICTRFGLRFEDYSDEWIPFTQLLMEVPFESVDQEQWLARNSAVE
                     LKDDRFSYFIAIREYRLRNSVAPFEYIRGQVRTIILNNRRNDFLQKLEDGIYNEAVSN
                     NTLKLY"
     gene            9037..10386
                     /locus_tag="PKK03_07435"
     CDS             9037..10386
                     /locus_tag="PKK03_07435"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX84271.1"
                     /translation="MKKNIILSCALAVMAVYGAAGQQVVESVVAVVGNEVIYLSDIEG
                     QVVQLKAERDPTPVEQLRCRIFEDQLVQKLFLDQARIDSIEVSTETVESDLNSRLTDY
                     VRRAGSEQALETYFNKSMAEIRKDLREMLTNQYIVQETQGTIAKGIAVTPEEVKKYYN
                     TIPRDSLPLVPRKVQLSIITVEPPDMDASKAEVRQRLLDLRSRIINGESFSTLAMVYS
                     EDPGSAPRGGELGFMTRGELYKPYAEVAWSLKQGVVSKIVETEAGFHIIQLIERKGDM
                     VNSRHILLKPKLSAEQTEWAILRLDSIANLVRSDSVSFTMAAMRYSSDKTTRANGGKM
                     VSENPAERVNWLTLDELPREINLVVRDMKLGEISDPFRSTDPKGNSVYCIVRLDEEIA
                     AHRVNMQEDYNYLSEAALREKQGRKYEEWVVDKIKRTYIRVSEDFRGCSFHYSGWLE"
     gene            10374..12107
                     /locus_tag="PKK03_07440"
     CDS             10374..12107
                     /locus_tag="PKK03_07440"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HNX84272.1"
                     /translation="MVRIARKITAGFISRSASRCALPPTGSRSRAGSYLQLQYLCNPG
                     RILLPLFFLLLSCHLRAQDTPGRRRVEILHSDSMKYEQSDGRTRNRLNGNVSLKHNDL
                     YMDCDSAWYYDDTNQVLAYSNIHIWQGDTIHIRGEYLSYNGNTRKAFLTDSVVLSDKE
                     MQLFTDKIDYDVNTQVANYDTWGRILNGDNTLTSREGIYYSDRKMLHFRDSVRIVNPD
                     YVMTADTMRYDTSREIVWFTGPTEAVGDSIYLYCENGWSDTRNDVSQLMKNAVLDNRE
                     QRITGDTLWYDEKSGKGEGFGRVTITDTTDNITGGGNYAWYVKRPEQFLLTGTPWFSL
                     FSDDDTLTLRADTLRAIPVEDTAGVSHRLLKAFHECRIFSREMQGKCDSLSYSFLDSV
                     IRLYDDPVVWSRAYQMTADSIALFTVNSKADHMEMYNSAFVVSEIDTSRYDQISGKNL
                     SGYFTDNKLTRIKVTGNGEAIYYVVDGEELVGVNRARSATIEILFEDSRIVEIIQNQS
                     PDGTLNPPLKVPDSEMRLDGFRWHPDLRPDRKGMGIDELFPPLPDEVREETPEEVREA
                     PPEAALKKPQR"
     gene            complement(12245..12466)
                     /locus_tag="PKK03_07445"
     CDS             complement(12245..12466)
                     /locus_tag="PKK03_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HNX84273.1"
                     /translation="MYWTLELASKLEDAPWPATKDELIDYAIRSGAPMEVIENLQEIE
                     DEGDIYESIEDIWPDYPSKDDFFFNEDEY"
     gene            complement(12558..13109)
                     /locus_tag="PKK03_07450"
     CDS             complement(12558..13109)
                     /locus_tag="PKK03_07450"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNX84274.1"
                     /translation="MKIYTKTGDKGETSVIGGVRLPKHHPRIEAYGTLDELIAWIGML
                     RSCEQSCVPREELIEIQSVLMSCCTVIATHPSAKVPDGIHIEDTVRLESSIDRMETDL
                     PGLQSFILPGGNNPAATANLARTVCRRAERAILRLNETEKTDPDVIRYLNRLSDYLFV
                     LSRCLVYSLGTEEVKWVSDKKAK"
     gene            complement(13181..13891)
                     /locus_tag="PKK03_07455"
     CDS             complement(13181..13891)
                     /locus_tag="PKK03_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX84275.1"
                     /translation="MDKVIEIQEIVKNYQVGTVIVRALRSVSLDIRRNEYVAIMGPSG
                     SGKSTLMNILGCLDTPTKGQYILNGTDVSKMEDDRLAEIRNKEIGFVFQTFNLLPRYT
                     ALENVTLPLIYAGVPRHEREDHAVKTLDLVGLGDRMTHKPNELSGGQRQRVAIARALI
                     NNPSIILADEPTGNLDSKTSYDIMGLLDKIHEDGNTVIVVTHEEDIARHAHRIIKLMD
                     GEVSADYINENRIEVRKK"
     assembly_gap    14119..14218
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14219..14835)
                     /locus_tag="PKK03_07460"
     CDS             complement(<14219..14835)
                     /locus_tag="PKK03_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84276.1"
                     /translation="MNLHTASAYAMLLLTARSTAGHGVHSPFMFRFITEVIGGRSDIA
                     IMREVESLRREMLSDRRVVMVTDLGAGSSVMRGSERTVRQIASAAAVPKREASLLTRI
                     AASLDLIRTGRPGSRQQPGGQTGAGDGSGEEPKIVLQKQAEAGDSQCAAVENETNKQK
                     QAESVDALWSGEGIPAPQPFRESDSGREDAGREDSGREDAGREDSG"
     gene            14908..>16686
                     /gene="recG"
                     /locus_tag="PKK03_07465"
     CDS             14908..>16686
                     /gene="recG"
                     /locus_tag="PKK03_07465"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNX84277.1"
                     /translation="MSELLDSEIKFLPGVGPKRAELLGAEADIHTFRDLIYYFPYKYV
                     DKTRFYKVRELDTDMQYVQVKGKIRRFTTEGVGKGRRLTADFYDETGIVKLVWFKGHK
                     WVISSYKPEKEYIIFGKPTLFSGAFNIVHPEIEEAEKRETRVSSALEAQYSTSEKMKD
                     HFLHSKAIGKLMATAFSMPALRFPETLPSSVTEPMKLMSLSDALFRIHFPDSNNDIDN
                     ARFRLKFEELFYIQLNLLRYKDNRKKSTHGFLFSVVGERLNSFYANNLPFALTEAQKR
                     VIREIRADLGSGRQMNRLLQGDVGSGKTMVALMSMLIGADNGFQSCLMAPTEVLAMQH
                     FNSISKFVSGIDANVALLTGSTKKSERARIEKGLLDGSIDILIGTHALIEENVQYKNL
                     GLVIIDEQHRFGVAQRAKLWQKSDCPPHVLVMTATPIPRTLAMTLYGDLDVSVIDELP
                     PGRKPIKTMAFTDAEREKVFGFMRRQIGDGRQIYVVYPLIKESESLDYKDLEDGLESM
                     ARAFPPPQYCISVVHGRMTSKDKEISMNMFRRGQAHIMVATTVIEVGVDVPNASVMVI
                     ESAERFGLSQLHQLRGRVGRGADQSYC"
BASE COUNT         3799 a         4263 c         4468 g         4056 t
ORIGIN      
        1 aggtctgaag gtctgaaggt ctgaaggtct gaaggtctga aggtcggcac ttttgattgc
       61 cacctgccga ctggtactgc ttactgccgc ctgacttttt cccctttcat ggagagctgt
      121 atacggttcc ggttgcggtc aaccgaaacg acgcggacag tgacatgctg atgcaattta
      181 acgatctttg ccggatcatc aacatagcgg tcagccatct gcgacacgtg cacaagaccg
      241 tcctgcttga cgccgatatc aacaaacgcc ccgaacctgg taatgtttgt gacaatgccg
      301 gggaccacca tcccctcttt cacatcatca attgaatgaa tatccgcaaa actgaattcc
      361 tgtatcgcct gacgcgggtc acgacccggc ctggccagtt cctgcatgat atccttcagg
      421 gttggcagcc cggcttcagc ggtaacatat ttctcaatca ttatggattt tcgccgggcc
      481 tcatccttta tcagggcagt gacagagcaa cccaggtcag cggccatctt tccgactatg
      541 tgatagctct ccgggtgcac ggccgaggcg tcgagcgggt tggcagcgcc tcgtatcctg
      601 aggaatcccg cagcctgttc aaaagccttc tctcccatcc gtttcacctt catcagttca
      661 gtgcgggagc ggaacggacc gttctccgtc ctgtgatcga cgatgttctg cgccatcttc
      721 gggccgatac ctgaaacata agtcagaagg tgcgggctgg cggtatttac ctccactccc
      781 acggcgttga cgcatgacat caccacctcg tcgaggctgg cctggaggcg ggtctgatca
      841 acatcatgct ggtactgccc caccccgatc gatttgggat ctattttaac gagttcagcc
      901 agcgggtcca tgaggcgccg tccaattgaa acggccccgc gcactgtcac gtcatgatcc
      961 ggaaactcgt cacgggcggt cttcgaggct gaatatatgg aagcgcccgc ttcgctcacc
     1021 acaaagacct taattcccga ttcagcaggg agatatgaac ggacgaactg ttcagtctcg
     1081 cgactggcgg ttccgttgcc gatagctacg gcctcaatgc tgaatttgtc aacaagtgaa
     1141 agcattttgc ttacgcttcg tgccgtctcg ttctgtgggg ggtgcggata gatcacgtca
     1201 tggtgcagca gggctccctg gcggtcaagg cataccagct tgcagcctgt tctctgtccc
     1261 gggtcaattc caagtacgtt cttttcaccc aaagggggtg ccagcagcag ttgccgcagg
     1321 ttttccgtga aaacgcgtat ggcctcctcg tcggcttttt ccttggatgt ctttctgaat
     1381 tccgtctcca ttgacggtgc caggagcctg ctccagctgt cggccacagc ctcgcgcacc
     1441 tgccgggctg aatcattgtt tgccttcaca aactgttttt caaccaggct ctgcgcctcc
     1501 tcctcagaag gcataatcgt aatcctgagg aatccctcct cctcgccgcg gcgcatggca
     1561 agcaacctgt gtgacgggca gcggcgcagc ggctctgacc agtcgaagta atcgctgaat
     1621 ttggctccct cctgttcctt tccctttaca accttcgagg ttatgacagc atcacgttca
     1681 tagagacgcc tgagcgatcc gcgaacctgt tcgttctcac tggcaatctc ggcgatgatg
     1741 tcacgtgcac cggacagggc agctgtcgta tcttccacat tatcacccac atactttcca
     1801 gcctctgctt cagtgtcacc ggtattctgt gcaagcagcc agagtgccag gggttcgagg
     1861 cctttctcgc gggcaacgga ggcgcgtgtc tttcttttcg gcctgaacgg caggtaaaga
     1921 tcctcgagct gtgacatggt cagggctgca ttgatctttg ccagcagttc aggagtcatt
     1981 ttctcctgtt cttctatcga tctgataact gcctccctgc gcttttccag ctccttaagc
     2041 ctctcatatt catcacgtat ctcagccagt tgcacctcgt tgagacttcc cgtcacctcc
     2101 ttgcggtatc ggctgatgaa gggtatcgtg gcgccgtcat caaggagcct cagagtattc
     2161 tcaacctgcc agtcgtgcag ctgaaatttt cgtgcaatag tcctgatatg taccgaagtg
     2221 tccatagatt attattcgat gtaaagaatc aatcctttca ggtattcgct ttcggggtgg
     2281 taaatattca cggggtggtc agccggctgt ccggtctggt gaagaatccg tacgttgcgt
     2341 ccggtattgg cggcggccac gaagaccgag cggcggaagt catcgcggct taccacctgc
     2401 gagcaggaga aggtaaacag cagaccgccg ggcctgattt tgtggatagc cagcgcgttg
     2461 agtctcctgt agccctgaag tgcctggtta agagcggtct gatgttttgc gaaggcgggg
     2521 ggatcaagaa taatgaggtc atattcgcgc tccattccgg ccaggtaggt gaaggcatca
     2581 gtggtgtagc agtggtggcg gggatcatcg ccgaagttga gcttcacgtt ttcatcggcg
     2641 agggccgtgg ccgccgcaga gctgtcgact gtatgcacaa gttcagcgtt tttcattgca
     2701 taaaccgaga agcctccggt atagccgaac atattaagga ccctcctgcc gttgctgtag
     2761 cggctcagca ggctgcgact gtcacgctgg tcaataaaga agcctgtctt ctgccccttc
     2821 tcccagtcga ccctgaacct gtagccgttt tcagtgacaa ccgcaccggg ttcaccgttt
     2881 tcaccaacga ggtagccgtt tgagggattc agccttgcca tgaacggaag cgttccctcg
     2941 cttttatcgt atactgccgt caccagtccg ccggtcacct caggcagcag ttgtgcaatc
     3001 atctccctga ttttccagaa tccggcagtg tgagcctgca tgactgcaat actgccatag
     3061 atgtcaacaa tcagtcccgg cagcccgtcg ccttcgccgt ggacaagtcg ccatacggtg
     3121 gtttcaggtt tgcctgcaat accggcattg atcctgaatg ccatggcctc ctcaagacgt
     3181 ttcctgaaga acccgtggtc aggattgcat ttcgtaaagg agaagatccg tactgcgatg
     3241 gatcccggag cccagtgacc cgttgccagg tatgttttgt cccatgcaaa aacatcaacg
     3301 acatcgcctt ctgacgggtt tccgttgact gaagctacgg ccccggagaa gatccagggg
     3361 tggtacctgc gtaccgactg ttccttgcct tttttaagga tgatgtttgc gggcattgat
     3421 agtggcttat tgataagttt ccttcccgtt aagggcaagg taaatgctcc ggcacaccca
     3481 ggcatctgtg gcagcgtaga gctgttgcgg ttcggtcagt gcgggagcct cccagtttga
     3541 aacctgctgc gactttgaga tagagtatcc gagaacgata gccgtgagtt tcttgaggcc
     3601 cagttcctgt attccatgat caaccacaac gctctgaagg tcaatgaatc cgtgcggttc
     3661 gaagggggta agtgcccgaa gggcttttat atcgtcgcgt atagcaacac ctgttttgac
     3721 tatgttcctg tcagagagca gcgctgccag ttcgggcgga aggccgatca tgttgagcct
     3781 gaagagccat gcccggtcag catcagcaag ctgcaggagc gccaccttgt atctcctccc
     3841 ctttttgaat gacggcctgg tttcggtatc aaatcccagc aatgagggtc tgcctatatc
     3901 gcccatgacc ctgcggaact tttccatatt gtcaaccagg gtcaccctcc cctcaaactg
     3961 cagcagaggc agtcttctca gttcatcatc ggtaatgctt ccgttatatt gtttcgtcat
     4021 cttcattctg ttcgttgcca gcaatcgtgt catcttcgga atagaggacg gtatggatcg
     4081 ccgtgagcac gttcagcagt ttctttcccc agaattcctc gaagttcatc agcacctccc
     4141 agacggcatc attcatcacc tcttcattac cgcttttata gctgacaatg aaatctttca
     4201 ggtcctggta aatatcactc aggttttcag aaatactgcc tgtatagagt ccgtcagcgg
     4261 ggtcacccac tgacacaatg accgggaaag ggtcattgtt gcccagtttt gaaagcaggt
     4321 tttcatgcat cctggtccag tcgaattccg tgacggtttt ttcgttgggt tcctcaagca
     4381 ccggttccag gagtggcaac agtgatcccc tcaggtatat gaacggcagg aggcgctgca
     4441 acaccttgag tatctctatg ccgttgattt cccgggttga ctccaggtat ttgcagtact
     4501 cgttcgctga agcggcaaac tcaaccacct ggcgggaata aataaccttt tcctcttttt
     4561 ccataatgaa cagatcaacg gggcaaaagt attcatttca gtcttaaaac cgggaatttg
     4621 ttgataaaag gaggcgggtt accttttcca ggtaagtctg tgatcactgt cataaaccag
     4681 tttctcatgg tcaaggagcc accggatgac tttgatgctt ttttcccgcg gaaggtcgat
     4741 ctgctcctcc agttcctcag gcctgagggg tttttcggca agcagttcct ttattttatc
     4801 gattatcaga tcgaactcat acttactcag ctcaagttcg tttctctcct ggcaggtgtc
     4861 acacagtccg catctgcccg attcctcgcc gaagtaagcg gccaggaaag cagggcggca
     4921 acgggttttg ttttcggcat agtcagtgat ccgggcaacc cgttcaatgt agttttcctt
     4981 caccttaagg tagagttcgg gtgagatgcg gaggtggctg cggtcaagcc gttcctcggt
     5041 gaaaatgacc agcgggctct tcttccccgg gataaaatga attattccct gtgatgagag
     5101 cctcaggagg tactggtaaa tggcttcgcg ggtaaggcct gacctggcag ccaggttttc
     5161 ttcattgacc ggaacaaatt cagagaacat gccgctgtag gtgcggagga gcagtttgat
     5221 gaacctgtca tactcctcgt tggctacctg gaacttgtaa aggtcatcgc gtgtcaccac
     5281 gaaatgcacc cgtgaaggat tgtttatctc atccgtaaat tcaaggtatc cctccttctg
     5341 gaggaagttg aggctgttgt atgtttcggt cacagagaac cggaatgccg aaacgaactt
     5401 ccacaggtcg aagtcgtgca cgctgttcct gcctgagccg accgggagct gatagaagtt
     5461 gccgatagct tcgtagacat cttttatccg ttccaccggc ggaaatttta tgcgtaccga
     5521 gtcagccagc tttctctttt catcctgaga ccagagcagc acggagaatg agggtttccc
     5581 gtcgcggcca gctcggcccg attcctggta gtatgcctcc acggagtcag ggcagtccca
     5641 gtggatgacg aacctgacat cggccttgtc aattcccatg ccgaaggcgt tggtagccac
     5701 tatcacgcgg gtttcgccgg cgctccatga ggtctgtttc ctgtcacgca tttcctgtgg
     5761 cagtccggcg tggtagaagt cggccttaat attgttagcc acaagcatct cggccacctc
     5821 ctttgccctt ttccggctgc tcacatagat tattccgctg ccctgttcct ttttgaggct
     5881 tcgcaggacg tatgtgttct tatcctccac ctcccttacc atataggtga tattgggtct
     5941 ccggaagctg gtgcggtgga ccttcgggtt cttcatcttc agtcttgaga tgatgtcatc
     6001 cacaacccgc ggggtggcag tcgccgtcag ggcaaggaat gggacatcct cattcatcat
     6061 atcccttatt tccgcgatat ggaggtatga gggtctgaag tcatatcccc actgtgatat
     6121 gcagtgggct tcgtcaacag ccacgagggt gaagttgaat ctcggcaggc gggccctgaa
     6181 catcggtgtc tgaagtcttt cggggctgac atacagcagc ctgtagtcgc cgtacaggca
     6241 gttatcaagg gatatttcca cttcttcgcg ggtcatgccg ctatgaatgg ccatagcttt
     6301 gatattcatt gtcttaagtc ttaccacctg gtctttcatc agtgctatca ggggtgtgat
     6361 gaccaggcat agtccctcac cggcaacggg aggcagctgg taggttaccg atttgccgcc
     6421 gccggtcggg agaaggccca ccacgtcaga tccggagacc actgactcaa tgatctcctc
     6481 ctgcatgggc ctgaaggacg tgaatcccca gtaccgtgcg agagtctccc tgtagatatc
     6541 cggcagcatg gttttgcagg ctgtttaata caaatttaat aactcaatct tacttttaac
     6601 cttttattga catttaagat atgcaggtgc ttagcaattt tttgtaattt tcgccccaag
     6661 taaaaaaaac ccttgcccga tgtcattctc tgaagataaa ctgctgtttg aaggtctgac
     6721 cttcgatgat gtgctgctcc ttccggccta ctcggaggtt ttgccgcgaa acgttgatct
     6781 caggtcaaag ttcacacgga acatcatcat caacactccc attgtttcgg cggccatgga
     6841 taccgtcacc gaggcagagc ttgcaatagc catcgcccgt gagggcggta tcggcgtgat
     6901 tcacaagaat atgtcagtca gtcagcaggc tgcccaggtg cggaaggtaa agcgtgcgga
     6961 gagtgttatg atttttgatc ctgtcaccat ctctgacggg gcctcagtgg ctgatgccct
     7021 ggcgctgatg aaggagttca agatcggcgg cattccggta attcatgaag acggtgcgct
     7081 ggcagggatt gtcaccaatc gtgatcttcg tttcgagact gactattcgc gtcgtatcag
     7141 tgagatcatg acacctcttg agaggcttat caccctcagt catcagacag atctggcggc
     7201 tgccgctgag atactgcaga agcacaagat agagaagctt cccattgttg atgagaaggg
     7261 aaggctgaag gggctggtga cctacaagga cattacaaaa gcaaaagaca ggcccaacgc
     7321 atgcaaggat gacaaggggc ggctgagggc cgccgccgca gtgggcattg cacccaatac
     7381 cctctccagg gttgaggcgc tggtgggcgc cggcgttgac gccatagcca ttgatacagc
     7441 tcacgggcat acgaagagtg tgatagaaat tctgagggag attaaaaaga actttccccg
     7501 taccgaggtt gttgtcggga acattggcac cgcggaggca gccaggaagc tggttgagga
     7561 gggcgctgat gcggtgaagg tgggtatcgg gcccggttcc atctgtacca caagggtagt
     7621 tgcaggggta ggtgtacctc agttgtctgc gatatacaac gtggcacgcg ccatagaagg
     7681 cagcggagtt cccgtgatcg ctgacggcgg catcaggtat tccggtgaca ttgtcaaggc
     7741 aattgccgcc ggagctgaca gcatcatggc agggtcacta tttgccggtg ttgaggagtc
     7801 acccggtgaa accatcatct acaacggcag gaaattcaag acctatcgcg gcatgggctc
     7861 ggtggaggca atgcagatgg ggtcaaagga caggtacttc caggacatgg aggatgacat
     7921 tcacaagctt gttcctgagg gtatcacggc gcgtgttccc tacaagggca ctctcaccga
     7981 ggtgatttac cagttccttg gcggcctgcg ctcagggatg ggttactgcg gggcacgtga
     8041 catagccaca ctcaagcgtg aagggcgttt tgtcagggta accactgccg gcgtcactga
     8101 gagccatccc catgatgtga ccatcacccg ggaggctcct aactacagcc gcaaaataaa
     8161 cgaatactga taatggtcag gcttatcatc cctctcctgc tccttgcagt tgtctcatgc
     8221 cgccatgccg gcactgatga gaacagaaac gctgtggcac gtgccggtga cagagtactc
     8281 tatcttgacc agataccatc agggcttatg acggaaggta tgagcaaggc agacagcacc
     8341 tcggccgtac agagctacat caggcagtgg gcccgtaagg agctcatggc cctgcgggcc
     8401 gaagagaacc tcactcccga atacaaggct gaggtcaaca ggcagctcaa tgagatgagg
     8461 aacaatctcc tgatttatca gtaccagcac cagatgatta tccagagaat ggataccacc
     8521 gttactgaca acgaagtgca ggattactat gtcagcaacc tcagtacctt taccctcact
     8581 tcgaacctca taagggctct ttacatcaaa gtaccggaag ggatgtcaga gcttgaccgg
     8641 atcaggaagc tttaccggtc acaggagtca gctgatatcc gggagcttga agacatttgc
     8701 acccggttcg ggttacggtt tgaagattac agtgatgaat ggatcccctt cacccagctt
     8761 ctcatggagg tgccgttcga gtcagttgac caggaacagt ggctggcaag gaacagcgcc
     8821 gttgagctga aggatgacag gttttcctat ttcatcgcaa taagggaata caggctgagg
     8881 aacagcgttg caccgtttga atatataagg ggacaggtca ggaccataat cctcaacaac
     8941 aggaggaacg atttcctgca gaagctcgaa gacggcatct acaatgaggc tgtgagcaac
     9001 aacacattga aactctatta gaagggattg aaaaggatga agaagaatat cattttatca
     9061 tgtgcgctgg cagtaatggc ggtgtacggt gcagccgggc aacaggtggt tgaatcggtt
     9121 gtggctgttg tgggcaatga ggtaatctac ctctctgaca tcgaggggca ggtggttcag
     9181 ctcaaggctg agcgtgatcc cactcccgtg gagcagctca ggtgccgtat ctttgaggac
     9241 cagctggtac agaagctttt ccttgaccag gccaggattg actctatcga ggtttcgacg
     9301 gagaccgtcg agtctgacct gaacagcagg ctgactgatt acgtaaggcg tgccggttcg
     9361 gagcaggccc tggaaaccta tttcaacaag agcatggcag agatccgcaa ggatttgcgg
     9421 gagatgctca ccaaccaata catagtccag gagacccagg ggaccatcgc gaaaggtata
     9481 gcagtcactc cggaggaggt aaaaaaatat tacaacacca ttccccgtga ctcgcttcct
     9541 ctggtaccga ggaaggtaca gctgagcatc ataacggtag agccgcctga tatggatgcc
     9601 agcaaggcag aggtgcgtca gaggctccta gacctgcgca gccgtatcat caacggtgaa
     9661 agtttcagca cattggcaat ggtgtattcc gaggacccgg gttcggctcc tcgaggaggc
     9721 gagttgggat ttatgaccag gggtgagttg tacaaaccat atgcagaggt agcatggagc
     9781 ctgaagcagg gcgtagtgtc caaaatagtt gaaacagagg ccggttttca catcatccag
     9841 cttattgaaa ggaagggaga catggtcaac tcgcgtcata ttcttttaaa accgaagctt
     9901 tcggccgaac agaccgaatg ggccatccta aggctcgaca gcatcgccaa cctggtccgc
     9961 tctgacagcg tatcctttac aatggctgcc atgaggtatt catccgacaa aaccactcgt
    10021 gccaacggcg gaaagatggt tagcgagaat ccggctgaaa gagtcaactg gctaactctt
    10081 gacgagttgc ccagggaaat taacctggtg gtaagagaca tgaaactggg agagatctcc
    10141 gatccgttcc gtagcacgga tccgaaggga aacagcgtct attgcattgt caggcttgac
    10201 gaggagatag ccgcccacag ggtcaacatg caggaagact acaactatct ttccgaagca
    10261 gctctgaggg aaaagcaggg ccgcaaatac gaggagtggg ttgttgacaa gatcaagagg
    10321 acgtacatcc gtgtcagcga agacttccgc ggatgcagct tccactattc cggatggtta
    10381 gaatagccag gaaaataaca gcaggattca tatcccgaag tgcttcccgg tgcgcattgc
    10441 cgcccaccgg ctcgcggagc agggcaggca gttatttaca gctgcaatat ctctgtaacc
    10501 ctggccggat tcttttgcct cttttcttcc tgcttctgtc atgccacctc cgggcacagg
    10561 acacccccgg ccgcaggagg gtagagatac tgcactctga ttcaatgaaa tatgagcagt
    10621 ctgacggccg tacacgtaac aggctgaacg gtaacgtcag cctgaaacac aatgacctgt
    10681 acatggactg tgacagtgca tggtactatg atgacaccaa tcaggtactg gcttacagca
    10741 acattcatat ctggcagggt gacaccatcc acatccgcgg tgagtacctt tcgtacaacg
    10801 gcaacacccg gaaggcattc ctgaccgaca gtgtggttct ctccgacaag gagatgcagc
    10861 tctttacaga caagattgac tatgacgtga acacccaggt tgccaactat gacacctggg
    10921 ggcgtatact caacggagac aacaccctta caagcaggga gggtatctat tactccgaca
    10981 ggaagatgct tcacttccgc gacagtgtca ggattgtcaa tcctgattat gtgatgacgg
    11041 ctgacactat gcgctatgac acgagcaggg agatagtctg gtttaccggt cccaccgagg
    11101 cagtcggaga cagtatttat ctatactgtg agaatgggtg gagtgacacc cgcaatgatg
    11161 tttcacagct tatgaagaat gcggtgcttg acaaccggga gcagcgcatc accggagaca
    11221 ccctctggta tgatgagaag agcggcaaag gtgaaggttt cggaagggta accataacag
    11281 acaccactga caacatcacc ggcgggggta attatgcctg gtacgtgaag cggccggagc
    11341 agtttttgct gacgggcaca ccctggtttt cactgttttc tgatgatgac acgctcaccc
    11401 tgagggctga caccctcagg gccattcctg ttgaagacac tgcaggggta agtcacaggc
    11461 tgctcaaggc attccatgaa tgcaggatat tcagccgtga gatgcagggg aagtgtgatt
    11521 ctctctccta ctcatttctc gactcggtca tcaggcttta tgatgatccc gttgtatggt
    11581 cacgggctta ccagatgaca gcagattcca tcgccctgtt tacagtcaac agtaaggctg
    11641 accatatgga gatgtacaac agtgcatttg tggtaagtga gattgatact tcacggtatg
    11701 atcagatcag cgggaagaat ctatcaggct attttactga taacaagctg accaggataa
    11761 aggtcaccgg gaatggtgag gccatttatt atgtagttga tggagaagaa cttgttggtg
    11821 tcaaccgggc gcgaagcgcc actattgaga tactgtttga ggacagcagg atagtggaga
    11881 taatccagaa tcagagtcct gacggcactc tgaatccacc cctgaaggta cctgactccg
    11941 agatgcggct tgacgggttc aggtggcatc ctgatcttcg gcctgaccgt aaagggatgg
    12001 ggattgatga gctgttccct ccgcttcctg acgaagtccg ggaggaaact ccagaggaag
    12061 tccgggaggc ccccccggag gcagccctga agaagcccca acggtagtcc cggaggaagg
    12121 cctgaggaag ccccagcggt agctcttagc tcgggaggca gaggggctca taaaagccga
    12181 agtgcccgga actgacgtcg gaatgaccgt tccaggcact tagtgtgctt atagcaaaag
    12241 gctgctagta ctcgtcttcg ttgaagaaga agtcgtcctt tgagggatag tcaggccaga
    12301 tatcttcaat gctttcgtag atatcaccct catcttcaat ttcctgaagg ttttctatta
    12361 cctccatggg ggcacctgac ctgatcgcgt agtcaatgag ctcgtctttt gtggcgggcc
    12421 atggcgcgtc ttcaagtttt gatgctaatt caagtgtcca atacataatt aagagagtta
    12481 ttttattacc cttctaagag tccgcaaata taaaattatt tagaataaaa aaagacttag
    12541 gaaagttttt acccggatta ttttgctttt ttatccgaca cccacttaac ttcttctgta
    12601 ccaagactat atacaagaca cctggacaat acgaagaggt aatctgacag tcggttgagg
    12661 tatcttatga cgtcagggtc ggttttttcg gtttcattga ggcggaggat tgcccgttcg
    12721 gcacgtcggc agacggtgcg ggcaaggttg gctgttgcag cgggattgtt acctcccgga
    12781 aggatgaatg actgcaatcc gggcaggtca gtttccatcc ggtctataga actttccagc
    12841 cggacggtat cttcgatgtg gatcccatca gggactttcg cggatgggtg ggtggcgatt
    12901 actgtgcagc atgacatgag caccgactgg atttcaatca gttcttcacg tggtacgcat
    12961 gactgttcgc aagagcgtag catacctatc caggcgataa gttcatcgag ggtgccgtag
    13021 gcttcgatgc ggggatgatg ttttggcagt ctgacgcctc cgatgacaga tgtctcgcct
    13081 ttgtcgccgg ttttggtgta gattttcatc gcttttacct gaccggagct ggtatggtta
    13141 tcaatacccg gccattctgc atcgggtcgt ctgttggagc ttacttcttt ctgacttcga
    13201 tgcggttttc attgatatag tcagccgaga cctcgccgtc catcagtttg atgatcctgt
    13261 gggcatggcg tgcgatatct tcctcgtggg taactacgat aactgtattg ccgtcttcat
    13321 ggatcttgtc aagcaggccc atgatgtcat atgaagtctt actgtcgagg ttaccggttg
    13381 gttcgtcggc gaggattatt gaggggttgt tgatcagagc cctggcgatt gccactctct
    13441 ggcgttgtcc tcctgaaagt tcgttgggtt tatgggtcat tctgtcgccc agtcccacca
    13501 ggtcaagtgt cttgacagca tggtcttcgc gttcatggcg gggtactccg gcgtatatca
    13561 gcgggagggt gacgttttcc agtgcggtat acctgggcag caggttaaag gtctggaaga
    13621 caaagcctat ctccttattg cggatttctg cgagcctgtc gtcttccatc ttgctgacgt
    13681 cggttccatt aaggatatac tgtccttttg tgggtgtgtc gaggcatccg aggatgttca
    13741 tcagtgttga ctttccggat ccggaagggc ccataatggc cacatactca tttcttctga
    13801 tatcgagtga aactgaacgg agagctctga cgatgactgt tccgacctgg tagtttttaa
    13861 ctatttcctg aatttcaatg actttatcca tgcctctttt attaaagttc cgaaattagt
    13921 tcaaattaag atataataat gttccgttgc tgttattttt tgttaatacc gcaccctgta
    13981 atgccccggg gtgatggatg ggatgcagaa cagcattccc gtacggagtg tttccagggt
    14041 agccgggacc gggctggcgg gcggcagcca aggatccggg accgggccgg cgggcggcag
    14101 ccaaggatcc gggaccggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
    14221 ggaatcctcc cgaccggcat cctcacggcc ggaatcctca cgaccagcgt cctcacggcc
    14281 ggaatccgac tccctgaatg gctgaggtgc cgggatacct tcccctgacc acagtgcatc
    14341 aactgattca gcttgcttct gcttatttgt ttcattttct accgcggcac actgcgaatc
    14401 tcctgcttca gcttgcttct gcagaactat tttcggctct tccccggacc catccccggc
    14461 accagtttgc cctcctggtt gttgccggct tcccggcctc cccgttctga tcaggtcgag
    14521 gctcgcagct attctcgtca gcagcgacgc ctcccttttc ggcacggcgg cagcagaggc
    14581 tatctgccgc acggttcgct cactgcctct catcaccgat gatcctgcac cgagatcggt
    14641 caccatcacc acccgcctgt ctgaaagcat ctcccgtctg aggctctcaa cctccctcat
    14701 tattgcaatg tcactcctgc ccccgatcac ctccgtgatg aaacggaaca tgaagggact
    14761 gtgcactccg tgacccgcag tacttcgggc agtcagcagg agcatggcat aagccgatgc
    14821 tgtatggagg ttcatttctt cagacagttc atttgaatcc cggtcaaaat taactatttt
    14881 cggtgacaat ctttttgacc gccagtgatg tctgaattgc ttgacagtga gatcaaattc
    14941 cttccaggag tgggccccaa acgggcagaa ctgctcgggg cggaggcaga catacacaca
    15001 ttccgcgacc tgatatacta ttttccttac aagtacgtcg ataaaacccg cttctacaag
    15061 gtcagggagc ttgataccga catgcagtac gtacaggtga agggcaagat ccgcaggttc
    15121 accaccgagg gggtcggaaa agggagaagg ctgacagcag acttctacga tgagaccggc
    15181 attgtaaagc ttgtctggtt caagggacac aagtgggtta tcagctcata caaacccgaa
    15241 aaggagtata tcatcttcgg caaacccacc ctcttcagcg gtgcattcaa cattgtgcat
    15301 cctgaaatag aggaggcaga gaagcgcgaa acacgagttt cctctgcact tgaggcacag
    15361 tatagcacct cggagaagat gaaggatcac ttccttcatt caaaggcgat cgggaagctc
    15421 atggcaacgg cattttcaat gccggcactc cgcttccctg agactctccc ctcttcagtc
    15481 accgaaccga tgaagctgat gagcctgtct gatgcccttt tcagaatcca tttccctgat
    15541 tcaaacaatg acatcgataa tgcccgtttc aggctgaaat ttgaggaact gttttacatc
    15601 cagcttaacc tgctgagata caaggataac cgtaagaaaa gcacacacgg gttcctcttt
    15661 tcggtagtcg gtgaaaggct caattcgttt tatgccaaca accttccctt tgcactcacc
    15721 gaggcccaga agagggtgat ccgcgagatc cgtgccgatc ttggcagcgg caggcagatg
    15781 aaccggctcc ttcagggaga tgttggcagc gggaagacga tggttgcgct tatgagcatg
    15841 ctgatcggcg ccgacaacgg gttccagagc tgcctgatgg cccccacgga agttcttgcc
    15901 atgcagcact ttaactccat tagtaaattt gtttcaggaa tcgatgcaaa cgtagcgctc
    15961 ctgacagggt cgacaaagaa aagtgagcgg gcaaggatag aaaagggact gcttgacggt
    16021 tcaattgaca tattgatcgg aacgcacgcg ctgatcgagg aaaacgtaca gtataagaac
    16081 ctggggctgg ttataattga cgagcagcac cgtttcggtg tcgcgcagcg cgcgaagctg
    16141 tggcagaaga gcgattgtcc tccgcatgtg cttgtgatga ctgccacgcc aatcccccgc
    16201 acgttggcga tgacccttta tggcgaccta gatgtctctg tcattgacga gttaccgccc
    16261 gggcggaagc cgataaagac aatggcattc accgatgccg agcgtgaaaa ggtcttcggg
    16321 ttcatgcgga ggcagatcgg ggacgggcgt cagatttacg ttgtttatcc attgataaag
    16381 gagtcggaat cacttgatta caaggatctc gaagatggcc tcgagagcat ggcccgtgcc
    16441 ttcccgccgc cacagtactg catcagtgtt gttcacgggc ggatgacctc gaaggacaag
    16501 gagatctcga tgaatatgtt tcgcaggggg caggcgcata taatggtcgc aacaaccgtg
    16561 attgaagtgg gtgttgacgt acccaatgcc agtgtgatgg ttattgagag tgcggagcga
    16621 ttcggactct cgcagttgca tcagttgcgc ggtcgggtgg gccgtggcgc agaccagtcg
    16681 tactgc
//
LOCUS       DAOPXU010000060        16590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_3688_length_16590_cov_12.347566, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000060 DAOPXU010000000
VERSION     DAOPXU010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16590
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3688_length_16590_cov_12.347566"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..2222
                     /locus_tag="PKK03_07470"
     CDS             51..2222
                     /locus_tag="PKK03_07470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX84278.1"
                     /translation="MPPPFLTRTDDWLTRRLSYPGADKHVIAQQKMYWISSVAVTSMI
                     LLLTILYHIFFPELRIIIYYGLFLSLVYMQGVIAPLLIRRVSVTWQIINQTIVALVTF
                     YTILKLGGIPYSGGLVLVGLALVFFTLNYKEKRHSVFIYSVYIITVILAGVLHPRLTV
                     PAEMTPQANISLYVINILWITGFALIFVLSFISQRVRLEELENIRLREIDEARKKLYT
                     NISHEFRTPLTVITGMNDLISTDPDQWLEKGSRTIDRNARILLNLVDQMLDLAKLEAK
                     AMPVNMIRADVILCIRYIVELFRSFAESKGIEMEFVSQHEELALDYDQDAIIKILSNL
                     ITNALKFTHPGGRVIIAAEQISDNKYEIRVSDNGPGITETFIPFVFDRFTREPATASQ
                     KTPGSGLGLALTRELVSLLNGTIRVESIYGEGSEFVVELPVTKTAKPGILNDRCQFGG
                     NRFHPESDSASKAGATTTLPHWDERNPILLIVEDNEDVISYLMTILADDYEVAAAADG
                     LEGFERACEIVPDIILTDVMMPGLDGIRMLDMVKNDPRTSHIPVVILTAKADVASRVE
                     GLARGADAYIAKPFNREELRAELRMLIRQRKLLCTRYSAAGMPPPDDNYNFKIEDKFI
                     KRINEVMTVHLADDRFDINSLCEQLNMSRTQLYRKFRSLSSQSPHDYYLKMKLQKARQ
                     MLTTSDLTVAEAAYRAGFKNVSHFSKAFTREFGINPSDIRR"
     gene            complement(2340..3350)
                     /gene="recA"
                     /locus_tag="PKK03_07475"
     CDS             complement(2340..3350)
                     /gene="recA"
                     /locus_tag="PKK03_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009161438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNX84279.1"
                     /translation="MEKKEINKEKLKALEVTLGKIEKDFGRGTIMKLGDHAVDNVQVI
                     STGSIGLDSALGIGGLPRGRVVEIYGPESSGKTTLAIHAIAEAQKAGGIAAIIDAEHA
                     FDRFYAEKLGVDVENLLISQPDNGEQALEITDHLIRSGAIDIIVIDSVAALTPKAEIE
                     GEMGDSKMGLQARLMSQALRKLTANINKTNTTCIFINQLREKIGVMFGNPETTTGGNA
                     LKFYASIRLDIRRTNQIKDGEDAVGNRVKVKVVKNKLAPPFKKAEFDIMFGEGISRTG
                     EIIDLGTEFNIIKKSGSWFSYGETKLAQGRDAVKKLLEDNPELAEEIKVKITEQLREK
                     AK"
     gene            complement(3369..3836)
                     /gene="bcp"
                     /locus_tag="PKK03_07480"
     CDS             complement(3369..3836)
                     /gene="bcp"
                     /locus_tag="PKK03_07480"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNX84280.1"
                     /translation="MTQLTAGMPAPHFEGTDQNGKTVKLSSFLGKKVVLYFYPKDDTP
                     GCTAEACSLRDNYNELLKKGFIVLGVSPDSEKSHAKFAGKYNLPFPLIADPQKKILSA
                     YGAYGEKLMYGKKVTGVIRTTFIIDEKGIIEKVIKKVDTKEHATQIFKMYDNV"
     gene            3899..5152
                     /locus_tag="PKK03_07485"
     CDS             3899..5152
                     /locus_tag="PKK03_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84281.1"
                     /translation="MHLRVINTGVVRAAAAMPVWLMLSVCPSLAQQGRAKPSDGEVQT
                     VKVRGDVTGVPYEYFRMLGEAARKQHEADSASLRAGQAVQQTRNAGTDQRERYYRKAM
                     QDSTRAAARKAEADSLLLAVEKAAGCPPEAPGPATIEETPASQLSRFDVLPGQAYTQS
                     NPVPVEPGMPGGLVYTIQIAAFKNDVTPSIFRGLTPVFGRKRQGSEALYYFAGLFRRL
                     DDARRALPEARGAGFPDAFIIALLDGTLVSLERAAHLEKEWGSRPLDGDSAVPGGTKP
                     AAGGTKSSAGGTRPEGGGAKPGATTSKHSAGASEPVAQTPPVPIGTLSFRAEVMRIDK
                     PVKPEVVQKIEVLAGNRGLDMVKNSNGETVFLIGNFITFESADEYVSLLVRNGYGSAR
                     VAAYVDRQEIPVEAARELVNKMPDD"
     gene            5145..5414
                     /locus_tag="PKK03_07490"
     CDS             5145..5414
                     /locus_tag="PKK03_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNX84282.1"
                     /translation="MTKRAPKPENNRRSEENLRSLMLINDDVNSFDHVIRSLVDVCGH
                     DEIQAEQCAVLTHIKGGCVIKIADARTVEKMRMKLRELSLDTVVI"
     gene            5425..5901
                     /locus_tag="PKK03_07495"
     CDS             5425..5901
                     /locus_tag="PKK03_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNX84283.1"
                     /translation="MSLGLIIFLIFVGLLLFIIEFMLIPGITIAGVGGAICLLTGIVF
                     AFVSFGTATGLVVLGITALVMVVLTVLMLRSNTWNKFMLKTAIDSKVDTVGAEEGKVK
                     PGDRGITVTRLAPGGKVLVNGEYFEAKSVDILIDPKQEIEVIRIDDNKLIVKPINK"
     gene            5904..6890
                     /gene="floA"
                     /locus_tag="PKK03_07500"
     CDS             5904..6890
                     /gene="floA"
                     /locus_tag="PKK03_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613657.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HNX84284.1"
                     /translation="MSALGIYVIIIVGAIILLWILLYFIPIGLWFQALVSGVRISLLQ
                     LIFMRWRKVPPSIIVGAMIEADKAGLKLNRDELEAHYLAGGHVAQVVHALVSANKANI
                     DLSFKMATAIDLAGRDVFQAVQMSVNPKVIDTPPVTAVARDGIQLIAKARVTVRANIK
                     QLVGGAGEETVLARVGEGIVSSIGSAHSHKEVLENPDRISKTVLEKGLDAGTAFEILS
                     IDIADIDIGKNIGAVLQMDQANADKNIAQAKAEERRAMAVALEQEMKAKAQEARAKVI
                     EAEVEIPLAMAHAFKSGNLGIMDYYRMKNIEADTSMRDSISKPPTSGGEKKK"
     gene            complement(6959..8233)
                     /locus_tag="PKK03_07505"
     CDS             complement(6959..8233)
                     /locus_tag="PKK03_07505"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HNX84285.1"
                     /translation="MKSFSVLPLLFLAFSLQAQVAFDDYFTDRTMRFDFMMAGNSTTT
                     KVYPVSFREEPFWGGSLINLIDPFGYGNFRYEIFDAVTGKLIYSRGFCTLYQEWQTTA
                     EAKQMERVFHEVATFPFPKNKVNFVLSIRGRDCHFSKLYETAIDPASYFITREKPDAS
                     LATRIAGSGDPHTSLDLAFIAEGYTSAEMEKFRNDVKRMAEFIFAEAPFDKYRDRINI
                     WAVEAPSQESGTDVPGEGIYVNTALSASYYTFDVDRYLTTQDIRRVNDYAASAPHDQI
                     IVLINSPRYGGGGVYNYYSAVTADHKLTPKVLTHEFGHGFAGLADEYYSSEVAYEEFY
                     PLDVEPWEPNITTRVNFDSKWKSLIAKSVPVPTPNEKRYIGVTGLFEGGGYSAKGIYR
                     PSYDCRMKSNEADSFCEVCQEALEKMILFYLK"
     gene            8323..9582
                     /locus_tag="PKK03_07510"
     CDS             8323..9582
                     /locus_tag="PKK03_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNX84286.1"
                     /translation="MFRSFDPVMAVREFLASLGMPASAVSLLSTVILVAAVGLLAWLS
                     YNFTRLILVRIFTRIVRRSRSAYDDKFLETRMFKRLAMIVPGVIVYYLASWMLRENPG
                     WLTFAHKTAALYIVIYATLTLTAFIEGWHQVWLMQPISTGRSIKPYVQIVKVLVAFAG
                     ILIMISVLFRVSLTSVFAGLGVATAVLAVVFKDTLLGLVASVQLSTNNMVKLGDWITI
                     PGRNIDGTVIDMTLYTVKVENFDKTILTVPTYALISESFQNWKGMEDSGVRRIKREIR
                     IDVRSIAFVTPELLDSISVRTHVAKWLSENNEDIEGLRNGGTTSLTNLGAFRAYMVEY
                     LRNHPHIDSMMPVMVRDMPPTESGMPVEIYCFSKINSWVPFESVQSSVVDFAYAVAGQ
                     FGLKLFQSPSGTDLEALRSVTNQNHKI"
     gene            9579..10799
                     /locus_tag="PKK03_07515"
     CDS             9579..10799
                     /locus_tag="PKK03_07515"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HNX84287.1"
                     /translation="MSLTYNEKLTAFIRAGIDDKPVSPGYDPFWQSLHSTGTELWLDT
                     GDMDEAEANWTAEMTALTTNNTLLNNEIQKGIYDSFVPRALEMVSHLSVSEQVKEIAF
                     ILNARHGLRLAKKFGGMVSVELHTDTAYDIDAIVSYGLRYRELCPEQFIVKVPYTPEG
                     LIGARLLHDMGVRINFTLEFSARQNALVTLVARPDYLNVFLGRIGAFIADNKLGDGSG
                     AGENAVLSSQSWVTRLSAETGSKTRQIAASLRNYTQLDLLAGTDVYTIPPKVAAAGRK
                     SLGGLFESRLTQRYDVPLNDDAAGLGIEKFWEVPKSVVELGRQLGKEVPAYGEEIVAK
                     AHEAGLGDMFPVMDPEEKARIAAEGKIPVLSSWKERILSGSLAPDTLLTLAGLASFTA
                     DQRQLDDRIRGILG"
     gene            complement(10952..11695)
                     /gene="rlmB"
                     /locus_tag="PKK03_07520"
     CDS             complement(10952..11695)
                     /gene="rlmB"
                     /locus_tag="PKK03_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNX84288.1"
                     /translation="MHSEKEFIFGMHPVMEAIRGGRQIDRVLVRQGLRGGQYHELMQV
                     VNEFAVPWQVVPQEKLDYITRKNHQGVIAWLSAIEFQDIENILPHIFEEGGDPLLLML
                     DGVSDVRNFGAIVRSALCFGAHAVIIPEKNSARISADAVKASAGALSTFPVCRVKSLT
                     KCLTYLKESGLKAVAATEKADRPVSAAGLTGPLVMILGSEEKGISRELLGLSDAQVSI
                     PITGAIGSLNVSVSAGILLYEVGRQRQAR"
     gene            complement(11695..13032)
                     /locus_tag="PKK03_07525"
     CDS             complement(11695..13032)
                     /locus_tag="PKK03_07525"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNX84289.1"
                     /translation="MTMRLIYKPAALAAVVTAAVLMTSCAVTKNVTDTKDMSYIYNPS
                     RSFFTPWISVYNEDAETSVLNISIRKSELYFNEANPEGVPMASMLVSVRLYDNTLGGA
                     LADTSTFKYDIKREETGGEYSFRTPLSARDGSSYTAEIKIIDLIRQRTQQLFVDFERT
                     GRYSGLNYKVRDHFTNNEVFSRAVRVDQYVNVLAPSLQPDTLWLFYYKAVTAIPPSPS
                     TILPEVTIAPEPEAIVPLAWSDTLPIMFPNQGIYLISVDSLIREGLVLCNFGPDHPSM
                     ARPETMIPPLAYIATPQEMDEMLTSEKPKLALDNFWLERTGSIERSKELIRIYYNRTL
                     FSNYYFTSYKAGWLTDRGMVYIMYGPPDKVYKNAEGESWGYKRPPVKSRWGSRYTYED
                     QYLWFNFRKQKSLFSDNDFVLNRAGTPVSYWDIAVARWREGKVFRLDNPQELR"
     gene            complement(13054..13812)
                     /locus_tag="PKK03_07530"
     CDS             complement(13054..13812)
                     /locus_tag="PKK03_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HNX84290.1"
                     /translation="MILFERKYPVNIFNTDLTGRLSPGALFSFFEDLAGRHAAELGWG
                     RDDLMASGGYFWALSRMLVKIERLPMAWDEVTLRTWPRGTETIFALRDLEMYDDTGRR
                     IAGASSSWVIVDYSTRKAQRPDKALSSLNLQFPEARALDTNARKVPSLPRGDHRLTRL
                     KVKLDDIDVNRHVNNARYVHWAVNCYDPEFISLHTPDTIEVNYLLEGHQDEMINILTA
                     PCDGEDNSYIHSVTRESDSTELCRLRMSWKENGQ"
     gene            complement(13799..16090)
                     /locus_tag="PKK03_07535"
     CDS             complement(13799..16090)
                     /locus_tag="PKK03_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNX84291.1"
                     /translation="MVSVNNVSLLFGSFVLLDEVSFLITRQERIGLTGRNGAGKSTLM
                     KIIAGLQEPTSGAVERPRELTVGYLEQQMKVSDTTTVLEEALTAFAELRSLEQDIDDM
                     TLEIAERTDYETDSYYQLLDRMHTAGERHSILGGSDYVALAGQTLNGLGFSAKEFNRP
                     TRELSGGWRMRVELAKILLRKPDLILLDEPTNHLDIESIIWLEGFLETYPGAVVLVSH
                     DRTFLDNVTRRTIEISLGKIYDYRVPYTKYTVLRRERREQQTAAYRNQQKMIDDTEKF
                     IERFRYKPTKAVQVQSKIKQLKKLERIEIEDEDTSAMNIRFSPAPRSGTVVVEADEAS
                     KRYGSHLVLDRVSMKIERGEKVAFVGRNGEGKTTLARMILGQVDFEGTLKRGHNVNIG
                     YFAQNQDELLNDDMTVFETVDRIAVGDIRTRLRDILGAFLFHGEDIDKKVKVLSGGER
                     SRLAIVQLLLEPYNLLLLDEPTNHLDIRSKEILKQALKKFDGTLIVVSHDRDFLDGLV
                     EKVYEFRHRKIRTHLGGIAEFLRARKIESLRELERKSPETGKGSGTVGKGSAVAGKGS
                     PLAGKGSAAAGKGSAAASKGSPLAGKGNPAAGKGNVAQVKGSPETGKGSPMTGKGRGT
                     QAKDSPPAGNGSPPAGNESPAAEKNGPPAANGNQGMTKVVSRAKPGITYQEKKENERV
                     HRRLQKQVTEAEAQITATEHEIAEMDARLASGDPAVISDPAFYSAYEEKKKRLEALME
                     QWENAHTELESFQAEYMNNNDTV"
     gene            complement(16193..>16422)
                     /locus_tag="PKK03_07540"
     CDS             complement(16193..>16422)
                     /locus_tag="PKK03_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84292.1"
                     /translation="GGLVTGSGTAGSGTAGGGGLVTGSGTAGSGTAGGGGLAAGSGTA
                     GSGTAGGGELVAGSGTAGGGEQNAGRRAKCG"
     assembly_gap    16423..16522
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3654 a         4299 c         4447 g         4090 t
ORIGIN      
        1 ggcgtaactt aagtaagatt ttgataatca atacctgatc tgttactgag atgccacccc
       61 cgtttctcac cagaacagac gattggctta cccgcaggct cagttacccg ggagcagaca
      121 agcatgttat agcccagcag aagatgtact ggatttcaag tgtcgcagtc acatcaatga
      181 tccttctgct tacaattctc taccatatct ttttcccgga actcaggata ataatatatt
      241 acggattatt cctctcactg gtttatatgc agggtgtcat cgcgcctctt ctgatccggc
      301 gggtaagcgt tacctggcaa attatcaacc agacgatagt tgcactggtt accttctaca
      361 caattcttaa acttggaggc attccctatt ccggcggact ggttctggta gggttggctc
      421 ttgtcttttt taccctcaat tacaaggaaa agcggcattc ggttttcatc tattctgtct
      481 atatcatcac agttattctt gcaggtgtgc ttcatcccag gctgaccgta cccgccgaaa
      541 tgactccgca ggccaacatc tcgctctatg tcatcaatat cttatggata acgggatttg
      601 cattgatatt cgtactgagt ttcatatccc agcgtgtcag gcttgaggag cttgaaaaca
      661 tacgtctcag ggagatagac gaagcacgga aaaaactcta tacaaacatt tcccatgaat
      721 tccgcacacc acttacggta attaccggaa tgaacgacct gataagcacc gaccccgatc
      781 aatggctgga aaagggaagc aggacaatag acaggaatgc ccggatcctg ctgaaccttg
      841 ttgatcagat gctggacctg gcaaagctcg aggcgaaggc aatgccggtc aatatgatca
      901 gggccgatgt catcctgtgc ataaggtata ttgttgagct gttcaggtca tttgccgaat
      961 caaagggaat tgagatggag tttgtctcgc agcatgagga gctggcgctt gattatgatc
     1021 aggatgcaat aattaagatt ctttcgaacc tgataaccaa cgctctgaag ttcactcatc
     1081 cgggcgggcg ggttattatt gcggctgaac aaatatccga caataaatat gaaatacgcg
     1141 tgagcgacaa tggcccgggt attactgaaa cctttatacc gttcgtgttt gaccgcttta
     1201 cgcgggaacc tgccacagcc agccagaaaa caccgggatc gggtctgggg ctggccctga
     1261 ccagagagct ggtctccctg ctgaacggca ccatcagggt tgaaagcatc tatggcgaag
     1321 gctcggagtt cgtggttgaa ctgcctgtca ccaaaaccgc aaaaccaggg atcttaaatg
     1381 acaggtgcca gttcggggga aaccggtttc atcctgaatc tgattctgct tcgaaagcag
     1441 gcgccaccac caccttgccc cactgggacg aaaggaatcc gatactgctg atagttgagg
     1501 acaatgagga tgttatcagt tacctgatga ccatccttgc tgatgattac gaagtggctg
     1561 ccgccgctga cgggctcgaa ggtttcgaaa gggcctgtga aattgttccg gatatcatcc
     1621 tgactgatgt tatgatgccc ggactggacg ggatcaggat gctggacatg gtcaaaaacg
     1681 acccacggac cagtcatatt ccagtcgtca ttcttacagc gaaggccgat gtggcttcaa
     1741 gggtggaggg gctggcacgg ggagcagatg cctacatcgc caagccattc aacagggaag
     1801 agctccgggc cgagctgagg atgctcatca ggcagcgcaa actgttatgc accagatact
     1861 ctgctgcagg aatgccacct ccggacgata actacaattt caaaatcgaa gataaattta
     1921 tcaagagaat caacgaggtg atgaccgttc atcttgcaga tgacagattt gacatcaaca
     1981 gcctctgcga acagcttaat atgagccgta cgcagttgta ccgcaaattc cggagcctca
     2041 gctcccagag tccgcatgac tactatctca aaatgaaact ccaaaaggca aggcagatgc
     2101 tgaccacttc agacctgacg gtggcagaag ctgcctaccg ggcgggattc aagaacgtat
     2161 cacatttcag caaggcattt accagggagt ttggaattaa tccgagcgat atccgcaggt
     2221 aattacggcc tgggagcaag tcataacgca tgccagccac gggtgcgcat caccggtcat
     2281 cataaataca ggtttaggtg aaaattgcaa tgaggagtga agtgcaggct tcaatccttt
     2341 tattttgctt tctccctgag ttgctcagta attttcacct tgatttcctc ggccagttcg
     2401 gggttgtctt caaggagttt cttcaccgca tcgcgtccct gcgcaagctt tgtttcgccg
     2461 taggagaacc atgagccgct ctttttgatt atgttgaatt ccgttccgag gtctatgatt
     2521 tcacctgtgc gggagatgcc ttcgccgaac ataatgtcaa actcggcttt tttgaatggg
     2581 ggagcaagct tgttcttgac aaccttcact ttgacgcggt tgccgacggc atcttcgcca
     2641 tccttgatct ggttggtgcg acggatgtcg agccttatgg aggcataaaa cttaagcgca
     2701 ttgccgcccg tagtggtctc gggattgccg aacatgaccc ctatcttttc cctgagctgg
     2761 ttgatgaaga tacaggtggt gttggttttg ttgatattgg cggtgagctt gcggagggcc
     2821 tgtgacataa gccttgcctg gagccccatc ttcgagtcgc ccatttcccc ctcaatctca
     2881 gccttgggtg tgagggcggc aacagagtct ataactatga tgtcaattgc tcctgacctg
     2941 ataaggtgat cggttatctc gagagcctgt tctccgttgt caggctgtga aatgagcagg
     3001 ttctccacat ccactcccag tttctcggca tagaacctgt cgaaggcatg ttcggcatcg
     3061 attattgcgg ctataccgcc tgccttctgc gcctcggcaa ttgcatggat agccagggtt
     3121 gtcttccctg atgactccgg gccgtatatc tccacgaccc ttccgcgggg cagtccgccg
     3181 attccgagcg ctgaatcgag gcctattgac ccggttgaga taacctgcac attgtcaacg
     3241 gcatgatcac ccagcttcat gatggtgccg cggccaaaat ccttttcgat cttgcccagc
     3301 gtcacctcaa gcgccttcag cttttccttg ttgatttcct tcttttccat ttacttattc
     3361 tttagcagtt agacattatc gtacatttta aagatctggg tggcgtgctc cttcgtgtca
     3421 acctttttta tcaccttctc aataattccc ttttcatcga taataaaggt ggtgcgtatc
     3481 acgccggtca cttttttccc gtacatcagt ttttcgccgt aggcgccata ggctgacaat
     3541 atcttctttt gaggatccgc tatcagcggg aagggaaggt tgtatttccc ggcaaatttt
     3601 gcgtgcgatt tctcactgtc aggactgact cccagcacga tgaatccctt tttcagaagc
     3661 tcattgtaat tgtctctgag actgcatgcc tctgcagtgc atccgggagt gtcatctttc
     3721 ggataaaaat agagtactac ttttttcccg aggaagctgc tgagcttcac ggtctttccg
     3781 ttctggtccg tcccctcgaa gtgcggggcc ggcatgcctg cggtgagctg agtcatgact
     3841 ttttgttatt tttgttatga ctggtaaagg tatgaagtta tcatctttca caaaatattt
     3901 gcacctgcga gttattaaca caggggtggt gcgggcggca gcagcgatgc cggtctggct
     3961 gatgctgtca gtgtgccctt cgcttgccca gcagggccgc gcgaaaccgt cagacggaga
     4021 agtacagaca gtgaaggtgc ggggtgatgt aaccggtgtc ccgtacgaat acttcaggat
     4081 gcttggtgag gctgcacgga aacagcatga ggctgattca gcttcactcc gggccgggca
     4141 ggctgtgcag cagaccagga atgccggcac tgaccagagg gagaggtatt acaggaaagc
     4201 aatgcaggac agcacccggg ccgctgcccg aaaagccgag gctgactcac tgctgcttgc
     4261 cgtggaaaag gctgcgggct gtcctcctga ggcgccaggg ccggcgacca ttgaggagac
     4321 ccctgcttct cagctcagcc ggtttgatgt tttacccgga caggcttaca ctcaaagcaa
     4381 tcctgtgcca gtggagccgg ggatgccggg cggactggtc tataccatcc agatcgctgc
     4441 cttcaaaaat gatgttacac cgtcgatttt caggggactc actcccgttt tcgggaggaa
     4501 gaggcagggg agcgaggcat tgtattattt tgcggggttg ttccgcaggc ttgatgatgc
     4561 caggagggct cttccagaag caaggggagc cggtttcccc gatgccttca tcatagcgct
     4621 tcttgacggc acactggtct cactggagag ggctgcccac cttgaaaaag agtgggggtc
     4681 acgtccgctg gatggtgatt cggctgtgcc aggcggcaca aaacctgcgg ccggcggcac
     4741 aaagtcatcg gccggcggca cgaggccgga aggcggcggc gccaagcctg gcgcgactac
     4801 ttcaaagcat tcggccggcg cctcagagcc tgtggcacaa actccgccgg tacccatcgg
     4861 taccctgtca ttccgggctg aggtgatgcg catcgacaaa cctgtcaagc ctgaagtggt
     4921 acagaagatc gaggtgcttg caggcaaccg cggcctcgat atggtcaaaa acagcaatgg
     4981 cgaaaccgtg tttctcatcg gtaattttat tacatttgaa agcgctgacg agtatgtctc
     5041 gctgctggtg cgtaatggct acggttccgc aagggtcgca gcctatgtgg acaggcagga
     5101 gattcctgtg gaagctgcaa gggaattggt aaacaagatg ccggatgact aaaagggcgc
     5161 cgaaaccgga aaataacagg agaagcgagg agaatctgag atccctcatg cttatcaatg
     5221 atgatgtcaa cagctttgat catgtcatca gatcacttgt tgatgtctgc ggccatgatg
     5281 agatacaggc agagcagtgc gctgtgctta cacacataaa gggcggatgt gtcatcaaga
     5341 tagcagatgc ccgcacagtt gagaaaatga ggatgaagct cagggaactg agccttgata
     5401 cagtagtcat ataaagaatt caacatgtct ctgggattga tcatatttct gatattcgtg
     5461 ggtctcctgc tcttcatcat agagttcatg cttatccccg gcataacaat tgcaggcgtt
     5521 ggcggcgcaa tctgcctctt gactgggatc gtattcgcat ttgtctcgtt tggtactgca
     5581 accggactgg tggtactcgg aataaccgca cttgttatgg tagtccttac tgttttgatg
     5641 ctgagatcca atacctggaa caagtttatg ctcaagacag ccatagacag caaggttgac
     5701 accgttggcg ctgaagaggg aaaggtaaag cccggcgaca ggggtataac agtcaccagg
     5761 ctggctccgg gcggcaaggt tctggtcaac ggagagtatt tcgaggcaaa gtctgttgac
     5821 atactcattg acccgaaaca ggagatagaa gtcatcagaa ttgacgacaa caaactgata
     5881 gtaaaaccaa taaataaata aatatgagcg cattaggcat ttatgtgata attatcgtcg
     5941 gggcaataat actgctctgg attcttcttt acttcattcc gatcggactc tggttccagg
     6001 ctcttgtatc cggcgtaaga ataagccttc ttcagctgat tttcatgcgc tggaggaaag
     6061 ttccgccgtc aatcatcgtc ggagcaatga tcgaggctga caaggccggg ttgaagctta
     6121 accgggatga gctggaggct cactaccttg caggcggaca tgtagcccag gtggtgcatg
     6181 cacttgtctc ggccaataag gcgaacatag acctgtcgtt caagatggca acagccatcg
     6241 acctggcagg ccgtgatgtt ttccaggccg tgcaaatgtc ggttaacccg aaggtgattg
     6301 acactcctcc cgtgacagct gtcgcccgtg acggtattca gcttatcgcg aaggcgaggg
     6361 ttaccgtaag ggccaatatc aagcagcttg tcggtggagc aggggaggag acggttcttg
     6421 cccgagtcgg cgagggcatc gtgtcatcga tcggttcggc gcacagccac aaggaagtgc
     6481 ttgagaatcc tgacaggata agcaagacgg tacttgaaaa gggtcttgat gcaggaacag
     6541 catttgagat tctctcgatt gatatcgctg acattgatat aggcaagaac attggcgccg
     6601 tgctgcagat ggatcaggcc aatgccgaca agaacattgc ccaggccaag gccgaggagc
     6661 gtcgtgcgat ggcagtggcc ctcgaacagg agatgaaagc gaaggctcag gaagcacgtg
     6721 ccaaggtcat tgaggccgag gtggagattc ctctggccat ggctcatgca ttcaagagcg
     6781 gcaacctggg tatcatggat tactacagga tgaagaacat cgaggctgac accagcatgc
     6841 gtgactccat ctccaaaccg cccacttcgg gaggagagaa gaagaagtaa tcaggatttt
     6901 cgattttcga tttgcgaatt gcgatttaac gggccattcg cagatcgcaa ttctttttct
     6961 atttcaggta gaaaaggatc attttctcaa gcgcctcctg gcacacctcg cagaatgagt
     7021 ctgcctcatt gctcttcatg cggcagtcat atgagggcct gtaaattccc ttggcggaat
     7081 accctcctcc ctcaaagagg ccggttacac cgatatatct cttttcattt ggcgttggta
     7141 cagggactga cttggctatc aggctcttcc atttggaatc gaaattcacc ctggtggtga
     7201 tatttggttc ccatggttcc acatcgagag ggtaaaactc ttcgtatgcc acctcagagg
     7261 agtaatactc atctgccagt ccggcaaaac cgtgaccgaa ctcgtgcgtc agcaccttag
     7321 gcgtaagttt gtgatcagcc gtgacggcgc tgtaatagtt atacactccg ccaccaccgt
     7381 acctggggct gttgatgagc acaatgatct ggtcatgcgg tgctgaggcg gcgtagtcgt
     7441 tgaccctgcg tatatcctgt gtggtcaggt accggtcaac atcgaaggtg tagtagctgg
     7501 cactcagggc agtattgaca tagattcctt cccccggcac atccgttccg gattcctggg
     7561 agggagcctc aacagcccag atatttatcc ggtctctgta cttgtcgaag ggtgcttcgg
     7621 cgaagatgaa ctccgccatc cgtttgacat cgttccggaa cttttccatc tcggcgctgg
     7681 tgtaaccttc ggcaatgaat gcgaggtcaa gtgaggtatg gggatcgccg ctgcctgcta
     7741 tgcgggtggc cagggaagcg tcaggtttct ccctggtaat gaaatagctg gcagggtcaa
     7801 tggccgtctc gtacagtttt gagaaatggc agtcgcgtcc cctgatgctc agcacgaaat
     7861 tgactttgtt tttcgggaaa gggaaggttg caacctcatg gaaaacgcgt tccatctgct
     7921 tcgcctcggc ggtggtctgc cactcctggt aaagggtaca gaagccgcgc gagtaaatca
     7981 gcttcccggt aacggcatca aaaatctcgt acctgaagtt gccgtaaccg aacggatcaa
     8041 tcaggttgat cagtgaacct ccccagaatg gttcctccct gaaggagaca ggatagactt
     8101 tggttgttgt gctgttgccg gccatcatga aatcaaacct catggtgcgg tcagtgaaat
     8161 aatcatcgaa agcaacctgg gcctgaagtg aaaatgccag aaacagaagc ggtaatacag
     8221 agaaagactt catgatttga cagttttata gtgtaaaggt actaaacagg cagccacaaa
     8281 aaaagataat tagtaaattc accggcgtaa aaaaagttaa taatgttcag atcattcgat
     8341 ccggttatgg cagtcaggga gttccttgcg agcctgggga tgccggcttc agccgtttca
     8401 ttgctgagca ctgtcatcct ggtggctgcg gtgggcctcc tcgcgtggct gtcatataat
     8461 tttacccgtc tcatcctggt caggatattc acccgcatcg taaggcgttc gcggtcagct
     8521 tatgacgaca agttcctcga aacgaggatg ttcaaacgcc tggcaatgat agtgcccgga
     8581 gtcattgtat attacctcgc aagctggatg cttcgcgaga atcccggctg gctcacattt
     8641 gcccataaga ccgctgcact ttatatagtg atctacgcta ccctgaccct caccgctttc
     8701 atcgagggat ggcaccaggt ctggctcatg cagcccatct caaccgggag gtcaatcaag
     8761 ccttacgtgc agatagtaaa ggttctggta gcttttgccg ggatactgat catgatttcg
     8821 gtactcttca gagtatctct tacaagtgtt ttcgcgggac tgggcgtggc aacagccgta
     8881 ctggcagtgg tattcaagga caccctgctg ggccttgtgg ccagcgtaca gctctcaacc
     8941 aacaatatgg tgaagctggg cgactggata acgattcccg gaaggaacat tgacggaacg
     9001 gttattgaca tgacactcta tacggtcaag gtcgaaaact ttgacaagac gatactcacg
     9061 gtgcccacct acgccctgat atcagaatct tttcagaact ggaaggggat ggaggattca
     9121 ggggtaagga ggatcaagcg cgagatccgc attgatgtac gctcaattgc ctttgtcacg
     9181 cctgaactgc tggactccat ctctgtgagg acccacgtgg cgaagtggct ctctgaaaac
     9241 aatgaagata tagagggact caggaatggc gggaccacct cacttacgaa cctcggggca
     9301 ttcagggctt acatggtcga atatctcagg aatcatcctc atattgactc catgatgcct
     9361 gtgatggtgc gtgacatgcc tcccacggag tcaggcatgc cggtggagat atactgtttc
     9421 agcaagataa attcatgggt tccgtttgag agcgttcagt catccgtggt cgattttgca
     9481 tatgccgtgg cgggacagtt cgggctgaag ctcttccaga gtccttccgg gacagacctt
     9541 gaggctctca ggagtgtgac aaatcaaaat cataagatat gagtttaacc tacaacgaaa
     9601 agctgaccgc ctttatcagg gccggtatcg atgacaaacc tgtttctccg ggatatgatc
     9661 cattctggca gtcgttgcac tcaacgggaa cagagctttg gcttgacacc ggtgacatgg
     9721 atgaagccga ggccaactgg accgcggaga tgaccgcgct taccaccaac aatacgcttc
     9781 tgaacaacga gattcagaag ggcatatatg attcctttgt tccgcgggcg ctggagatgg
     9841 tcagtcatct ctcagtcagc gagcaggtga aggagatagc cttcattctt aacgcccgtc
     9901 acggcctccg cctggcgaag aagttcggag gtatggtaag tgttgaactg catactgata
     9961 cggcatatga cattgatgcc atcgtctcat acggacttcg ctacagggag ctatgcccgg
    10021 aacagttcat cgtaaaggtt ccgtatactc ctgaaggact tattggcgcc cggttattgc
    10081 atgacatggg ggtgcggatc aactttaccc tcgagttcag cgcccgccag aatgcgctgg
    10141 tgacactggt ggccaggcca gactacctga atgtgttcct cggaaggata ggggcattca
    10201 ttgctgacaa caagctgggt gacggcagcg gcgcaggcga aaacgcagtt ctttcatcac
    10261 agtcatgggt tacacggtta tccgctgaga cgggctcgaa gacaaggcag atagctgcca
    10321 gcctcaggaa ttacactcag cttgacctgc ttgcaggaac cgatgtctat actatccctc
    10381 cgaaggtggc agctgccggc aggaaatcgc tcggcggtct ctttgagagc aggcttacac
    10441 agcgctatga tgtgcctctg aacgatgatg cggcaggact gggaattgaa aaattctggg
    10501 aggtgccgaa gtcggttgtt gagctgggca ggcagcttgg gaaggaagtt ccggcctatg
    10561 gtgaggagat agttgcaaag gcgcatgagg caggcctggg tgacatgttc ccggtgatgg
    10621 atcctgagga gaaggcgcgc attgctgcgg aaggaaagat accggtgctg tcatcatgga
    10681 aggagcggat tctctcagga agtctcgccc ctgacaccct gctgaccctt gccggcctgg
    10741 catcgttcac tgctgaccag aggcagttgg atgaccgtat ccgcgggata ctcgggtgag
    10801 aaaattgcga attgcgagtt gcgaattgcg attttgcgat tggcgcataa agcattatat
    10861 tacagagcgt cacaaatcac aagtcacaaa tcgcaaatct cgaatcgcaa gtctgcactg
    10921 ctaactgctg actggtactg cctgctgact atcacctggc ctgcctctgc cggccaacct
    10981 catagagaag tatccccgct gaaacggata cattaaggga accaatggca ccggtgattg
    11041 gtatggaaac ctgcgcgtct gacaatccca gcagttcgcg gctgatcccc ttctcttccg
    11101 aaccgagaat cataaccagc ggaccggtca gtccggcagc gctcaccggg cggtcagcct
    11161 tctcagtggc agccacggct ttcagtcctg actccttcag gtaggtcagg cattttgtca
    11221 ggctctttac ccggcatacc gggaaagtgc tgagggctcc tgctgatgcc ttgacagcat
    11281 cggcagatat tcttgctgaa ttcttttcgg ggatgatcac cgcgtgggct ccgaaacaga
    11341 gcgctgaccg cactatggct ccaaagttgc gcacgtcact gacgccgtca agcatcagca
    11401 ggagcggatc accaccctct tcaaaaatat ggggcaggat attctcaata tcctggaatt
    11461 ctatggccga cagccatgct atcaccccct ggtggttctt gcgggtgata taatcaagct
    11521 tctcctgcgg caccacctgc catggcacgg caaactcgtt caccacctgc atcagctcat
    11581 ggtactgtcc gccgcggagt ccctggcgga ccagcacacg gtctatctgc ctgcctcccc
    11641 tgatggcttc cattacaggg tgcatcccga aaatgaattc cttttcgctg tgtatcatcg
    11701 cagttcctga gggttatcga gcctgaaaac cttgccttcg cgccaccgcg caacagctat
    11761 atcccagtag ctgacaggtg tcccggcacg gttcaggaca aaatcattgt ctgaaaaaag
    11821 gctcttctgc ttgcggaaat taaaccagag gtactggtcc tcatatgtat agcgcgagcc
    11881 ccagcggctt ttgacgggag gtctcttgta accccagctc tctccctcgg cgttcttgta
    11941 gaccttgtca ggcggaccgt acattatgta gaccatgccc ctgtcagtga gccatccggc
    12001 cttgtaggag gtgaagtaat agtttgaaaa gagcgtgcgg ttatagtaaa tcctgatcag
    12061 ctccttcgat ctctcaatgc ttcctgtacg ttcaagccag aaattatcga gcgccagctt
    12121 cggtttctct gatgtaagca tttcatccat ctcctgcggt gtggcgatat atgccagcgg
    12181 agggatcatt gtctccggcc gggccatgga ggggtgatca ggaccgaagt tgcagaggac
    12241 gagaccctcg cggatcaggc tgtcaacgga gatcagataa atgccctggt tggggaacat
    12301 gatgggaagc gtgtctgacc aggccagtgg aactattgct tcaggttccg gcgctattgt
    12361 cacctcggga agaatggttg acggggacgg ggggattgcc gtaacggcct tgtaatagaa
    12421 cagccacagc gtgtcaggct gcagcgacgg agcgagaacg ttgacatact ggtcaaccct
    12481 caccgcgcgg ctgaatacct cattattggt aaaatggtca cgtaccttgt agttgagccc
    12541 gctgtacctg cctgtacgct cgaaatccac gaacagctgc tgggtgcgct gcctgatgag
    12601 gtcaatgatc ttaatttcag cagtgtatga gctgccgtca cgtgcagaca agggggtccg
    12661 gaatgaatat tcaccgccgg tctcctcacg tttgatgtca tacttgaagg tcgatgtatc
    12721 tgccagggct cctccgagag tattgtcata gagccttacc gagacgagca ttgaagccat
    12781 cggtacgcct tcggggttcg cctcgttgaa gtagagctca ctcttgcgga tgctgatgtt
    12841 cagaacagat gtctctgcat cctcattgta tacggagatc cagggggtaa aaaaactccg
    12901 cgacgggttg tatatatatg acatgtcttt tgtgtcagtg acgttcttgg tcactgcaca
    12961 tgatgtcatc agtacggccg ctgtcacaac cgcagcgagc gcagccggtt tatagatgag
    13021 acgcattgtc atggcataaa ggtaataaag atttcattgt ccgttctctt tccagctcat
    13081 tctcagacgg catagctcgg tgctgtcgct ctcacgggtc acggagtgta tgtatgaatt
    13141 atcctcaccg tcgcagggag cggtaaggat atttatcatc tcgtcctgat gtccttcaag
    13201 gaggtagttc acctcgatag tgtcaggggt atggaggctg atgaattccg ggtcatagca
    13261 gttcaccgcc cagtgcacat atctcgcgtt gttcacgtgc ctgtttacgt cgatgtcgtc
    13321 aagtttgacc ttcagccttg tcaggcgatg atcaccccgg ggaagcgatg gcaccttgcg
    13381 tgcgtttgta tcaagggccc tggcttcggg aaattggagg ttcaggctcg agagcgcctt
    13441 gtcaggacgc tgagccttgc gtgtgctgta atcgactatc acccatgacg atgaggcacc
    13501 ggcgattctg cggcccgtgt catcatacat ttcaaggtcg cgcagggcga agatggtttc
    13561 ggtgccgcgt ggccatgtgc ggagggtgac ctcgtcccag gccatcggga ggcgttctat
    13621 cttcaccagc attcgcgaga gcgcccagaa gtacccgccc gatgccatca ggtcatcgcg
    13681 gccccagccc agctctgcgg catgacgacc cgccaggtcc tcgaagaagc tgaagagtgc
    13741 accgggactg agccgtccgg tgaggtcggt attaaagatg ttaaccgggt atttcctttc
    13801 aaacagtatc attgttgttc atatattccg cctggaagct ctcgagctct gtatgcgcgt
    13861 tctcccattg ttccatcagt gcctcaagcc tttttttctt ctcctcgtat gcgctgtaga
    13921 aggccgggtc acttatcact gccgggtctc ccgatgcgag gcgtgcatcc atttctgcta
    13981 tttcgtgttc cgtggccgtt atctgagcct cggcctctgt tacctgtttc tgaagccggc
    14041 ggtgcacgcg ttcgttttcc ttcttctcct ggtacgtaat gccgggtttt gcccgggaca
    14101 ccaccttcgt catgccctgg ttgccatttg cggctggcgg cccgtttttt tccgctgccg
    14161 ggctttcgtt tcctgccggc gggcttccgt ttcctgcagg cgggctgtct ttagcctgcg
    14221 tcccgcggcc tttacctgtc atggggcttc cttttcctgt ctccgggctt cccttcacct
    14281 gcgccacgtt tccttttcct gctgccgggt ttccctttcc tgccagggga cttcctttgc
    14341 ttgctgccgc gctgcctttt ccagctgccg cgcttccttt tcctgccagg ggacttcctt
    14401 tgcctgctac cgcgcttccc tttccaactg tcccgcttcc ttttcctgtc tccgggctct
    14461 tcctctcaag ctccctgagc gactctatct tcctggccct gaggaactcg gcgatccctc
    14521 cgagatgcgt tctgatcttc ctgtggcgga actcgtacac tttctcaacc agaccgtcaa
    14581 ggaaatccct gtcgtgagaa accacaatta atgtgccatc gaattttttc agcgcctgct
    14641 tcagtatctc ctttgagcgg atgtcgaggt ggttggtcgg ctcgtcgagg agaagcaggt
    14701 tatatggctc cagcagaagc tgcactattg ccagccgtga ccgttctccg ccggagagga
    14761 ccttcacctt cttgtcgatg tcctcaccat ggaagaggaa tgctccgagg atatcgcgga
    14821 ggcgggtccg gatgtctccc acggcaatcc tgtctaccgt ctcgaagacc gtcatatcgt
    14881 cgttcagcag ctcatcctgg ttctgggcga agtaaccgat attgacattg tgtccgcgtt
    14941 tcagtgttcc ctcgaaatcg acctggccga ggatcatcct tgcgagtgtc gtttttcctt
    15001 cgccgttccg gcccacgaaa gcaacctttt ctcctctttc gatcttcatt gagaccctgt
    15061 cgagcaccag gtggctgcca tagcgcttcg aggcttcatc tgcttccacc accaccgtcc
    15121 cggatctcgg ggcgggtgag aagcggatgt tcatcgcgga ggtatcctcg tcttctatct
    15181 caatgcgttc cagcttctta agctgtttga tcttgctctg cacctgcacc gccttggtgg
    15241 gcttgtacct gaaccgctcg atgaatttct cggtgtcgtc gatcatcttc tgctggttgc
    15301 ggtaggctgc ggtctgctgc tcgcgccgct cgcgccgaag tacggtatat tttgtgtatg
    15361 ggacccggta gtcatagatt ttgccgaggc ttatctctat tgtgcggcgg gtgacattgt
    15421 cgaggaatgt gcggtcatgc gacaccagca caacagcccc gggataggtc tcaaggaacc
    15481 cctcgagcca tataattgat tcaatgtcaa ggtggttggt gggctcatcg agaagtatca
    15541 ggtcaggttt gcggaggagg atctttgcca gctccacgcg catgcgccag ccaccgctga
    15601 gttcacgggt gggacgattg aattccttcg cgctgaaacc gagtccgttg agcgtctgtc
    15661 ctgccagcgc cacataatcg cttcctccca gtatgctgtg ccgttcgccg gctgtatgca
    15721 tccggtcgag aagctggtag tagctgtcgg tctcgtagtc agtccgttcg gcaatctcga
    15781 gggtcatgtc atctatatcc tgttccaggc tcctgagttc ggcgaaagcc gtaagggcct
    15841 cctcgaggac ggtggtggtg tcggagacct tcatctgctg ctcgaggtag cctacggtga
    15901 gttcgcgggg ccgctcgacg gcgccggagg tgggctcctg gaggccggcg atgattttca
    15961 tcagcgttga tttgcccgcg ccgttcctgc ctgtcagacc gatccgctcc tgccgggtaa
    16021 tgaggaatga gacctcatcc aggaggacga agctgccgaa gagcagtgat acgttgttta
    16081 cggagaccat ctggccggtt tttcaaggcg caaagataaa taaaatcgtg gtttggtggt
    16141 gagggcagga gggatgcacg gcatgcattg cactctcccc gcttttccgc cttcagccac
    16201 actttgcccg ccgcccggcg ttctgttccc cgccacccgc tgttccgctc ccggccacca
    16261 gttccccgcc gccagctgtt ccgctcccgg ccgttccgct ccccgccgcc agtcctccgc
    16321 cgccagctgt tccgctcccg gccgttccgc tccccgtcac cagtcccccg ccgccagctg
    16381 ttccgctccc ggccgttccg ctccccgtca ccagtccccc gcnnnnnnnn nnnnnnnnnn
    16441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16501 nnnnnnnnnn nnnnnnnnnn nncgcctgat actcaatctc acatttttcc gttataattg
    16561 cagataattc tgaaagtcat gaaaagcaaa
//
LOCUS       DAOPXU010000061        16045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_3882_length_16045_cov_14.853096, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000061 DAOPXU010000000
VERSION     DAOPXU010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16045
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3882_length_16045_cov_14.853096"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="PKK03_07545"
     CDS             complement(<1..1315)
                     /locus_tag="PKK03_07545"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNX84293.1"
                     /translation="MKRILTITVLAAFSLFSAAQVSSEKSVPADPAYRIGKLENGLTY
                     YIRHNTEPAGRASYYIIQNVGAILEQDNQNGLAHFLEHMAFNGTTHFPGKTLLSTLEK
                     HGVAFGRNINAYTSFDETVYNLSDVPVDKPGLIDTCLMILADWSDFLTLDEEEIDKER
                     GVILEEWRSRRNAQWRMMTQMLPVMYEGSMYAKRDIIGDTAVIKNFDPETLRAFYHDW
                     YRTDLQAIAVVGDFNVDDVEAKIKTIFSPIPAIENPLSRPENLLTSREGTKYLLVTDP
                     EAPRTEVGLMIIDREPDKRKRDLDYIRDGYVTSLMNNMMNNRFNEIVQKGTPPFVAGG
                     LSYSSDLPRNYNALTLAAYANEGQEAKGFEAALTELERARRHGFTQGELNRARAEMLS
                     DFENLYKQKDKISNDEWASKIRDHFLTGEPLPSLDLQYDYYKKILP"
     gene            complement(1423..2037)
                     /locus_tag="PKK03_07550"
     CDS             complement(1423..2037)
                     /locus_tag="PKK03_07550"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNX84294.1"
                     /translation="MHIAIAGNIGSGKTTLAGLLSKHYGWQAHYEDVDDNPYLNDFYE
                     DMQRWSFNLQIYFLNSRFSQVLEIKKAKHTIVQDRTIYEDAYIFAPNLHAMGLMSTRD
                     FENYFTLFKLMSSLVEPPDLLLYLRASVPTLVNQIQKRGREYESSIRLDYLKRLNERY
                     EAWIESYKLGRLLILEADYYDFPENKEHLSEVIDRINAELHGLF"
     gene            complement(2114..3628)
                     /locus_tag="PKK03_07555"
     CDS             complement(2114..3628)
                     /locus_tag="PKK03_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNX84295.1"
                     /translation="MPFIPSVIKWINTKRLSQIDLFRKYPAETQQEVLTRLVSASKDT
                     EWGREHGYGSVTSYEQFCRNVAVQSYDDMIPWVERLRAGEKNVLWPGEVKWFAKSSGT
                     TSTKSKFIPITRESLEDTHYRGAKDCLVLYTSLNPETRIFLGKGLTLGGSHQINSFSN
                     NSLFGDLSAILIENAPAYADLIRTPPARIALIEDFEQKMKMITEKTVDMNITSISGVP
                     SWYLVLIRHILKTTGKSNLHQVWPNLELFIHGGVSFAPYREQYKKLLPDPKMHYMETY
                     NASEGFFAIQDDLTDSSMLLMLDYGVYYEFIPADEFGTDNPRTLSIGEVERGVNYAIV
                     ISTDGGLWRYAIGDTVEFTSLYPHKLRITGRTKFFINAFGEEVIIDNAEKALEKACHG
                     TNAHISEYTAGPVYMGDESRGAHEWVIEFDREPDDPEHFIEILDTTLKALNSDYEAKR
                     YRDLTLARPRVIVVPKGTFYKWFRERDKLGGQNKMPRLSNSREFVESVLKTAGL"
     gene            complement(3669..4391)
                     /gene="recO"
                     /locus_tag="PKK03_07560"
     CDS             complement(3669..4391)
                     /gene="recO"
                     /locus_tag="PKK03_07560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNX84296.1"
                     /translation="MLTRTKAILLHHVRYSDNSLIAHFYTQEYGRLSVMVKGISSKRG
                     TARFNYFQPLNIFNTELYYYENRELHNLKEMMLAYIPKRIHGDIHRTTVALFISELLH
                     NVIREEDVNKMLFDFIESSVISLDEATSGISNFHLWFLVAFTDYAGIGPTRTDMTGCY
                     FDMLTGQFTPLQPMHPDYLDPAGARVLNLLLQMPAEDLASLRLSGEERTELLAGMLKY
                     YRLHLPGIREMRSLQVLRDIFR"
     gene            4495..5883
                     /locus_tag="PKK03_07565"
     CDS             4495..5883
                     /locus_tag="PKK03_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNX84297.1"
                     /translation="MESASKDQLLRRIARLVRQNKELEEQVKKLEREKERLLETTEKY
                     RLLVENNELKGVRVAEEEKSLKFNMVTVLFADIQSFSNLTGEMDPAVVMDELDEIFFE
                     FDRIIKKYHIEKIRTIGDTYMCAGGIPAKNITNPVEVVMAAVELRQFLRDFEAARRGD
                     RRIWNLKIGIHTGPVSATVSGRTRVSYDIKGRAVNTASRIQGVSESDSILISVMTYEL
                     IKEFFSCEYHGKLPVKYMGNIQMYQVVGLLPELADDSNGAVPNKSFRVKFGLIQLTDI
                     QEVILDKLEKELPDFLFYHNVKHTVDVVTEVELIGWAEGCSDEEILLLKTAALFHDAG
                     HTVSYDDHEYQGTLLAKEMLPAYGYSEDQLERICRIIMSTKLPPRPGDLLEQIICDSD
                     LDYLGRSDFIPVSNTLYEELKAQDKITNLNDWNKMQVKFISGHQYFTGTARHLREVNK
                     KLQIERIKSLIT"
     gene            complement(5897..8203)
                     /locus_tag="PKK03_07570"
     CDS             complement(5897..8203)
                     /locus_tag="PKK03_07570"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNX84298.1"
                     /translation="MKRIISLAILALASLLASGQGPVGSWDDHLPYSRSYSITAGGGK
                     IYSSTGSAVLVHDPGSGATSSLSRVSGLTETAIALVAWCEAEESLIIVYRSTGIDILK
                     NGIITSIPDIKNKYIPGLKEIYGVTVNGSRALLTASFGIVVIDIRGRYVADTWRPGPD
                     GETNSVNEAVILGDRVYAATARGVWSAPVSRQGLSYYGNWELLDDLPEPTSEYGKIAT
                     AGTGLMICKPGTTGPASSADSLFLVSQGQPATLITTEPAGTIRALDGDGSRVIVSLSS
                     SLRILSPQGAVTREIPGYGWAGTSPAGALATGQDLWVADASVGLVSTTNYTDFRNHTI
                     DGPYTANVADIVFSGNSFCVTGGTVDNAWGNVYRPLQVFAGSGNSWESHILYGEADRD
                     AMRAAIDPSNEKHLFVSSWGNGLYEFLDGELVKNYNQYNSPLSSILPGENYSRICGLA
                     WDRSGNLWMTQTGVQGNLKALTPDGNWIITQVNLNMTVAGDMVIDRNQYIWVLLPRGN
                     GLLVYDPAGTPSNTSDDRYIRMQVQDADGHTMNNLFSIAADHDGNIWVGTDMGPAVFY
                     NPGRVFTSELRASRIKIPRNDGSGLADYLLGTETVTALTVDGANRKWFGTLSSGAFLM
                     SDDGRKELAHFTTANSPLLSDNIVKITVNGVTGDVWFGTSEGIISYRGEATSGNDDFS
                     GIYAFPNPVREDFEGVVTITGLVGNSSVKITDISGNLVYETKSLGGQAVWDLRNYRSQ
                     RVATGVYLVFCANEDGTLAGVTKMLVIN"
     gene            complement(8215..8811)
                     /locus_tag="PKK03_07575"
     CDS             complement(8215..8811)
                     /locus_tag="PKK03_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HNX84299.1"
                     /translation="MELVFATNNKHKIREISDLLDKNFRILGLADLGITEDIPEEAET
                     LEENALFKARYVHEKTGLNVFADDTGLEVSSLGGAPGVYSARYAGESKSFDDNIEKLL
                     REMMDLSERSARFRTVIALIIDGSEYLFEGTVEGEIIRERRGSGGFGYDPVFLASGYD
                     LTFAEIPLSEKNRISHRAKAMRKLIGFLSDQNRAGTRK"
     gene            9116..10819
                     /locus_tag="PKK03_07580"
     CDS             9116..10819
                     /locus_tag="PKK03_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNX84300.1"
                     /translation="MIDTATAVPVLRYRDGIIFTMGNDTAVEYSYFMAGPDSEWTEWT
                     DRAYKEYTSLDAGKYIFMYRYRVKYAGVGTGEGSFRFRVRPPWYFSLLALIIYPLLLL
                     AGGIMMRRRTHRLYTERQSRLEQLIAERTEELQHEKEKSDSLLANMLPKGTAEEIMSK
                     GKADKRKYNFVTVLFSDIQGFTKIAEEMNPETLIDELDRFFFHFDSVAEKYRIEKIKT
                     IGDAYMCAGGIPERNRTNPVEVVLAALEMQKYMQVMKNDPARPAARFWDIRIGIHTGT
                     VVAGVVGHKKITYDIWGDTVNTASRMESSGEAGKINISGTTYEFVKEYFTCDYRGRMP
                     VKYKGDLDMYFVTGIRPELREADGSPNRKFLAKIEMIRILDVEEHVFTRYSEIASPDL
                     FFHSADYIRSVSLQADLLARAENLSDEDYIHLRLASVFIYFGYAFDYNDPEAAALKRA
                     GEILSFYGFDPHTLEAVRELIASASSPEQTSMAGRVLHDAVYDFTGRVDFISMVDKLY
                     REEKAYGKTGDPKAWFRELASRIGENGFLTETARRLRSVAEDEQLRALREFAGGRLNN
                     T"
     gene            10877..11893
                     /locus_tag="PKK03_07585"
     CDS             10877..11893
                     /locus_tag="PKK03_07585"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HNX84301.1"
                     /translation="MVNLDWNNLPFGYIKTDFNVRCHYKNGQWGKPEVSDSEYINIHI
                     AATGLHYGQEAFEGMKAYRGRDGKVRLFRWDENAKRLISSANGIHMAPVPIELFREAV
                     FTAVRLNEKFVPPYGSGASLYVRPVLYGSGAEVGVKPASEYTFIVFVTPVGPYFKGGV
                     KPVNMLICRDVDRAAPLGTGNIKVGGNYAASLRAIVKAREGGYGNALFLDALEKKYLD
                     ECGPANFFGIKNNTYVTPKSVSILPSITNKSLIAIAESMGIKTERRRIPVEELAEFEE
                     TGACGTAAVISPIAKIFDPETGKVYEYCKDGKPGPVSMKLFNKLVGIQYGDEPDEFGW
                     MTEV"
     gene            12006..12884
                     /locus_tag="PKK03_07590"
     CDS             12006..12884
                     /locus_tag="PKK03_07590"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNX84302.1"
                     /translation="MAKSTGGISRYRLGSSYATLVVSVSLVLFLLGILGMVLINARQL
                     SDYFRESLSFTIMLKEDAREADIRMLQKELDAKPYVKQTEYVSKDEAAVKLRNELGED
                     FLDFLGYNPLMPTIDVYLQARYTHPDSVIKLEKVIADYPVVDEVYYQESILNLINDNV
                     RKISVFLLVISLLLFLIALTIINNTIRLSIYSKRFLINTMQLIGANRSFIRKPFLLKS
                     LFFGFLAALIAIGLMMGLMYLIEKEFFTLFTYENINLFLLLCLVLAAAGILINGISTF
                     FAVNRYLGMHEDKLYY"
     gene            12891..13139
                     /locus_tag="PKK03_07595"
     CDS             12891..13139
                     /locus_tag="PKK03_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HNX84303.1"
                     /translation="MVAKKEEEKKTGFALGRENYKLMAIGFAVIIVGFILMAGGRSDD
                     PKVFSDDIFSFRRVTLAPLIVLAGFIFEIYAIMKRPRE"
     gene            13173..13949
                     /locus_tag="PKK03_07600"
     CDS             13173..13949
                     /locus_tag="PKK03_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNX84304.1"
                     /translation="MNWYEALIFGLVQGLTEFLPVSSDGHLEIVKFLFGGVKESFLFS
                     VVVHGATVLSILVVFWKDILKLLAGVFRFKMNEETVYVLKLIVSMIPVAIVGFTLKDQ
                     VESLFVADMDITGSFLLVTALFLLVGHFVPKRNKPISYGGAFVIGIAQALATLPGLSR
                     SGSTISTGLMLGNSKDELARFSFLMVIVPILGANFIELITTDAGTVNGVFFPLVIAFV
                     AAFVSGYAACRWMINIVRKGKLGWFALYCVLAGLTLIIFA"
     gene            13955..14674
                     /gene="truB"
                     /locus_tag="PKK03_07605"
     CDS             13955..14674
                     /gene="truB"
                     /locus_tag="PKK03_07605"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNX84305.1"
                     /translation="MKRCSDFEDGEVLLFQKPLYWTSFDLVNKVKLAIRHHCGLKKLK
                     VGHAGTLDPLATGLMILCTGKATRRVEEFRDLDKEYIAVVRFGQTTPSFDLESEVDAE
                     YPSGHITEEFVREKLRGFLGEQDQEPPLFSAKFVDGKRAYELARKKIEHKLDSVKVVF
                     RELELVEYSGVDATVRIVCSKGTYIRAFARDLGQALGSGGHLRSLVRSAIGDFRLEKA
                     LSVEEFKFFLENLKHSENSFV"
     gene            14702..15751
                     /gene="queA"
                     /locus_tag="PKK03_07610"
     CDS             14702..15751
                     /gene="queA"
                     /locus_tag="PKK03_07610"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNX84306.1"
                     /translation="MKLSQFKYTLPQELIALYPSKNRDESRLMVVHRDTGEIEHKVFK
                     DIVEYFRERDVLVFNNTKVFPARLYGNKEKTGAEIEVFLLRELNREQRLWDVLVDPAR
                     KIRIGNKLYFGEDDLLVAEVIDNTTSRGRTLRFLFDGTYEEFKQTLYSLGETPLPKFI
                     NRPVEPEDNERYQTIFAKHEGAVAAPTAGLHFSRELLKRLEIVGVEFAEITLHVGLGN
                     FRNVDVEDLTKHKVDSERIIISEDASVLINKAKERKSRICAVGTTVIRTLESSVSTSG
                     MVKPFNGWTNKFIFPPYDFRVPDMLVSNFQLPYSTLLMMVSAFAGYNLLFDAYRTAVK
                     EKYRFGTYGDAMLIL"
BASE COUNT         3868 a         3657 c         4277 g         4243 t
ORIGIN      
        1 cgggcaggat tttcttgtag taatcatact ggagatcgag cgaaggaagg ggttctccgg
       61 tgaggaagtg atctctgatc tttgaagccc attcatcatt gctgatcttg tctttctgct
      121 tatagagatt ctcaaaatcg gagagcatct cagctctggc cctgttcagt tccccctgtg
      181 taaatccgtg ccggcgggca cgttccagtt ctgtcagggc agcttcaaat cctttggcct
      241 cctgtccttc attggcataa gcagccagtg tcagggcatt atagttcctt ggaaggtctg
      301 aagagtagct cagtcctccc gcgacaaaag gaggtgttcc tttctgcaca atctcattga
      361 aacggttgtt catcatgttg ttcatgagtg aggtgacata tccgtcacgg atatagtcca
      421 ggtccctctt cctcttgtcg ggttcacggt caattatcat cagtccgact tctgtcctcg
      481 gggcctcggg atcagtaaca agcagatatt ttgtcccttc cctggaagtt aggagatttt
      541 caggcctgga cagagggttc tcaatggcgg ggataggaga gaagattgtt tttatttttg
      601 cttccacatc atcaacgtta aagtcgccca ccacagctat ggcctgcaga tcagtacgat
      661 accagtcgtg gtagaaagcc ctcagtgtct ccgggtcaaa gtttttaatc accgcagtgt
      721 cgccgatgat atcccttttg gcatacattg atccttcata cataactggc agcatctgtg
      781 tcatcattct ccactgggcg tttcgcctgc ttcgccactc ttccaggatg acacctcgct
      841 ccttgtctat ctcctcttcg tcaagagtca gaaaatcaga ccagtcggca agaatcatca
      901 ggcaggtatc gatcaggccc ggtttgtcaa ccggcacatc gcttaggtta tatacggttt
      961 cgtcgaagct ggtgtaggca ttgatgtttc ggccgaaggc caccccatgc ttctcaaggg
     1021 tgctcagaag ggtttttccc gggaagtgag ttgtgccgtt gaatgccata tgttccagga
     1081 aatgggcaag gccgttctgg ttgtcctgtt ccaggatggc gcccacgttc tggatgatgt
     1141 agtagctggc cctgcctgcc ggttccgtat tgtgacggat gtagtaggta agtccgtttt
     1201 caagctttcc gatccggtaa gccggatcag ccggaacgct tttttccgat gatacctgtg
     1261 ctgcactgaa aaggctgaaa gcagctaaaa cagtaattgt taaaattctt ttcataatct
     1321 ttacatggtt gaatttatga gacaatgaca tcacttgatt gatcaaaatt aaataaaaaa
     1381 accatcgcac tgatggtttt ttagatttct ttcaggtggt atctagaaca gcccgtgcag
     1441 ctcggcattg atcctgtcga tgacctcact gagatgttcc ttgttttcgg ggaagtcgta
     1501 atagtcagct tcgagtatca ggagtctgcc cagtttgtaa gattcgatcc atgcctcgta
     1561 gcgctcattc agtcttttga ggtaatcaag tctgattgag ctttcgtact cacgtccgcg
     1621 tttttggatc tggttgacaa gtgtgggtac ggatgccctg aggtagagca ggaggtcagg
     1681 cggttccacg agtgagctca tcagcttgaa aagagtgaaa tagttttcga agtcacgtgt
     1741 tgacatgaga cccatggcgt gcaggttggg ggcgaagatg tatgcgtctt cgtatattgt
     1801 gcggtcctgc actatggtgt gttttgcctt tttgatttcg aggacctggc tgaagcggga
     1861 gttcaggaag tatatctgga ggttgaatga ccatctctgc atgtcttcat agaagtcatt
     1921 caggtaagga ttgtcatcga catcctcata atgagcctgc cagccgtaat gttttgaaag
     1981 cagtccggcc agcgttgtct tgcctgatcc gatgttgcct gctatggcta tatgcataag
     2041 aaatgactgt taaaatccgt gtttaaatat atgaaaaagg gagggagtag ttctctctgt
     2101 tgataaaaag atttcagagg ccggctgttt tcaggacgct ctcaacgaac tccctgctgt
     2161 ttgacagccg tggcatcttg ttctgtccgc cgagcttatc cctctccctg aaccatttgt
     2221 agaaggttcc tttgggaaca actatcaccc tggggcgggc aagtgtcagg tccctgtacc
     2281 tttttgcctc ataatcagag ttcagggctt taagggttgt gtcaagaatc tcaatgaaat
     2341 gctcggggtc atcgggttcc ctgtcgaatt cgatcaccca ttcgtgtgcc ccgcggcttt
     2401 cgtcacccat gtagactggc cctgccgtat attcgctgat gtgtgcattc gtgccatggc
     2461 atgccttttc gagggccttt tcggcattgt cgattataac ctcttcgccg aaggcattaa
     2521 tgaagaactt tgttcgtccc gtgattctca gtttatgagg ataaaggctg gtgaactcga
     2581 cagtgtcacc tatggcgtat ctccacaggc cgccgtctgt tgagattaca attgcgtagt
     2641 ttaccccccg ttcaacctcg ccgatgctga gtgtacgcgg attatccgtc ccgaattcat
     2701 ctgcggggat gaactcatag taaaccccgt agtcaagcat tagcagcatt gagctgtctg
     2761 tcaggtcatc ctgtatcgcg aagaaaccct cggaagcgtt gtaggtctcc atataatgca
     2821 tcttcgggtc cggcagaagc tttttgtact gctcacgata gggtgcgaaa ctgacgccgc
     2881 catggatgaa taactcgagg ttgggccata cctggtgaag gttcgactta ccggttgtct
     2941 tcagtatatg ccggatgagg accaggtacc acgagggcac tcctgagatg ctggttatat
     3001 tcatgtcaac cgtcttctca gtgatcattt tcatcttctg ctcaaagtcc tcgataaggg
     3061 ctatacgtgc agggggcgtg cgtatcaggt cagcgtatgc tggggcgttt tcaatcagta
     3121 ttgcagagag atcgccgaag agagagttgt tgctgaaact gtttatctgg tggctgcctc
     3181 cgagagtaag tcccttgcca aggaagatcc ttgtttccgg attgagagag gtatacagca
     3241 cgagacagtc ctttgccccc cggtagtggg tgtcttcaag cgattcacgc gtgatgggga
     3301 tgaatttgct ttttgtgctg gtggtccctg atgacttggc aaaccacttg acctcacctg
     3361 gccagagaac atttttttcg ccggcccgga gcctctcgac ccatggaatc atatcgtcgt
     3421 atgactgtac ggcgacgttc ctgcagaact gttcataaga ggtcactgag ccgtaaccgt
     3481 gttccctgcc ccattcggta tcctttgaag ctgaaactaa cctggtcaga acctcctgct
     3541 gcgtctctgc ggggtatttc ctgaaaaggt caatctgtga gaggcgtttg gtatttatcc
     3601 atttaataac agaagggatg aatggcatcg ggtcagtttt gttcaaagat agctaaagcg
     3661 cgactatttt atctgaaaat atctctcagt acctgcaggg agcgcatctc cctgattccc
     3721 gggaggtgga gcctgtagta cttcaacatc ccggccagca gctctgtacg ctcttcgccg
     3781 gacaatctca gcgaggccag gtcttcagca ggcatctgca ggagaaggtt caacacccgg
     3841 gctccggcag ggtccaggta atcagggtgc attggctgca acggggtgaa ctgcccggtg
     3901 agcatatcga agtaacagcc ggtcatatca gttcttgtcg ggcctattcc tgcgtagtca
     3961 gtgaaggcaa cgaggaacca gagatggaag ttgctgatcc ctgaagtcgc ctcatcaagg
     4021 ctgattactg atgactcaat gaaatcaaag agcattttgt taacatcttc ttccctgata
     4081 acattgtgaa ggagctcact aatgaacaat gcaacggtgg tccggtggat atccccgtga
     4141 atcctttttg ggatataagc cagcatcatc tccttcaggt tgtgcaactc cctgttttcg
     4201 tagtaataaa gttccgtatt gaagatgttc agcggctgga aatagttgaa ccttgcggtg
     4261 ccccgctttg atgatatgcc ctttaccatt accgaaaggc gcccgtattc ctgcgtatag
     4321 aaatgagcta taagcgagtt gtctgaatat ctcacatggt ggagcagtat ggcttttgtc
     4381 ctggtcagca tagcccgaaa tgtcacctca aaagtattaa agtttcataa cagcatactg
     4441 cgaacagaga ctctgagttt attagaatag aattattatt tcaggtcagc caagatggaa
     4501 tcagcatcca aagatcagct tctcaggcga attgcaaggc ttgtgcgcca gaacaaggag
     4561 ctggaggagc aggttaagaa acttgagcgt gagaaggaga ggcttcttga aaccaccgag
     4621 aaatacaggc ttcttgttga gaacaatgag ctgaagggag tcagggtggc cgaagaggaa
     4681 aagagtctca agttcaacat ggtcacagtc ctttttgctg acatccagag tttctcgaac
     4741 ctgaccgggg aaatggaccc tgcagtggtg atggatgagc tggatgagat tttctttgag
     4801 tttgaccgga tcatcaaaaa atatcacatt gagaagatca ggaccatcgg cgacacttac
     4861 atgtgcgccg gagggatacc tgcaaagaac atcaccaacc cagtggaagt ggtgatggct
     4921 gctgtggagc tgaggcagtt tctacgtgat tttgaagctg ccaggagggg agacaggcgc
     4981 atctggaacc tgaagatcgg gattcacacg ggacctgtgt ctgccaccgt ttcaggcagg
     5041 accagggtct cctatgatat caagggcagg gcggtgaaca cggcaagccg gattcagggt
     5101 gtgtctgaaa gtgattcaat cctgatctcg gttatgacct atgagcttat caaggagttc
     5161 ttcagttgcg agtatcacgg caagcttccc gtaaaataca tgggtaacat ccagatgtac
     5221 caggtggtcg gtctcctgcc cgaactggct gatgacagca acggcgctgt tcccaacaag
     5281 tcattcaggg tcaagttcgg tctcatccag ctaactgaca tccaggaagt gatacttgac
     5341 aagctggaga aggaactgcc tgacttcctc ttctatcaca acgtcaaaca caccgttgat
     5401 gtggtcaccg aggtggaact catcggttgg gctgagggat gttctgacga ggagatactg
     5461 ctgctgaaga ccgctgcgct attccatgac gccggccata cggtctcata tgatgatcac
     5521 gagtaccagg gtaccctgct ggcgaaagag atgttgccgg cttacgggta cagtgaggac
     5581 cagttggaaa gaatctgcag gatcataatg tcaaccaagc ttccgccgag gcccggtgat
     5641 ctcctcgaac aaatcatatg tgactcagac cttgattacc ttggtcgcag tgattttatc
     5701 ccggtgtcca atacgcttta cgaagagctc aaggcacagg acaaaatcac caatctgaac
     5761 gactggaaca agatgcaggt gaagttcatc tcggggcacc agtattttac cggcacggcc
     5821 cgccacctca gagaggttaa taaaaagctt cagattgaga ggatcaaaag cctgataact
     5881 taataccggt aacagctcaa ttgatcacca gcattttagt cacgccggcc agggttccgt
     5941 cttcatttgc gcaaaacacc aggtagactc ctgttgccac tctctgtgac ctgtagtttc
     6001 gcagatccca cactgcctgc ccgccaaggg attttgtctc atagaccagg ttgccgctta
     6061 tatctgttat cttaaccgat gaattcccga caaggcctgt tattgtcacc accccttcga
     6121 aatcttccct taccggattc gggaaggcat aaatgccgga gaaatcatca tttcctgatg
     6181 ttgcttcgcc tcgatacgat ataatgcctt cagaggtgcc gaaccacaca tcaccggtga
     6241 ctccgttaac agttattttt acgatattgt cagaaaggag agggctgttg gcggtggtga
     6301 agtgagccag ctccttcctg ccatcgtcag acataagaaa cgcgccggaa ctgagagttc
     6361 cgaaccattt ccggttggcg ccgtcgacgg tgagagctgt caccgtttcg gtaccgagga
     6421 gataatcagc aagtccggaa ccgtcgtttc ttggtatctt gatgcgtgag gctctcaatt
     6481 cagaggtgaa gaccctgccc ggattataga agactgccgg gcccatgtct gttcccaccc
     6541 atatatttcc gtcatggtcg gcggcgatcg aaaagagatt gttcatggtg tgcccatcgg
     6601 catcctggac ctgcatcctg atatacctgt cgtcggaagt atttgaggga gttcctgccg
     6661 ggtcatagac aagaagtccg ttaccgcggg gcagtaacac ccatatgtat tgattccggt
     6721 caatgaccat atctcctgcc actgtcatgt taaggttcac ctgtgtgatg atccagttgc
     6781 cgtccggggt gagtgctttc aggttgccct gcactcctgt ctgggtcatc cagaggttgc
     6841 ccgaccggtc ccaggcaagg ccgcagatgc gcgagtagtt ctctccgggc aggatgcttg
     6901 acagcgggga gttatactgg ttgtagtttt taaccagctc gccatcgagg aattcataga
     6961 gaccgttgcc ccatgatgaa acgaagaggt gcttctcatt ggagggatcg attgcggccc
     7021 gcattgcatc cctgtcagct tcgccgtaga gaatatggct ttcccaggaa ttacccgagc
     7081 cggcaaaaac ctggagcggc ctgtagacat tcccccacgc gttgtcaacc gttcctccgg
     7141 tgacacagaa gctgttaccg gaaaagacaa tgtctgcaac gttggcagta tagggtccgt
     7201 cgatggtgtg gttcctgaaa tccgtgtagt ttgtggtcga taccaggccc actgaggcgt
     7261 cggccaccca cagatcctgg cctgttgcca gtgcgccggc cggggaggtt cctgcccatc
     7321 catatccggg tatctcccgg gtgacggcac cctgcggaga cagaattctc agagaagagg
     7381 agagtgacac tatcacgcgg ctgccgtctc cgtcaagtgc cctgattgtc ccggccggtt
     7441 cggtggtaat cagggtggca ggctggccct gtgagacaag aaacagggaa tcagctgatg
     7501 aagcaggtcc ggttgtgccc ggtttgcata tcatcaggcc ggttccggca gtagctatct
     7561 tgccgtattc tgatgttggt tccggaaggt catcgagcag ttcccagtta ccataatagg
     7621 agagaccctg gcggctcacg ggggcggacc atactcccct ggcggtggcg gcataaaccc
     7681 ggtcaccaag tatgacagct tcgttcacgc tgtttgtttc gccgtcaggc ccgggtctcc
     7741 aggtatcggc tacgtacctg ccgcggatgt caatcacaac aattccgaag ctggctgtca
     7801 gcagggccct gcttccgttg acagtcactc cgtagatctc cttcaggcca ggtatgtact
     7861 tgtttttgat atcagggatg ctggtgatga tcccgttttt aaggatgtct atccccgtgc
     7921 ttcgatagac gatgataagc gactcttccg cttcgcacca tgcaaccagt gcgatagctg
     7981 tctccgtcag tcctgatacc cttgagagtg acgaggtagc gccgctgccc ggatcatgga
     8041 caagcaccgc ggaaccggtt gaggagtata ttttcccgcc accggcggtg attgaataag
     8101 acctgctgta aggaaggtgg tcatcccatg atcctacagg tccctgtccg gatgccagga
     8161 gagaggccag ggcaagaatt gcgagtgaga tgatcctttt cattttatgg cctgttactt
     8221 tcgggtgcca gcccggttct gatcactgag gaatccgata agcttcctca tggctttcgc
     8281 cctgtgcgag atcctgtttt tttcgcttaa gggtatctcg gcaaaagtga ggtcatatcc
     8341 cgatgcaagg aagacgggat catatccaaa tccgccgctg cctcttcttt ccctgatgat
     8401 ttcgccttca accgtaccct cgaagagata ctccgagcca tcaataatca gtgcgattac
     8461 tgttctgaac cgggcgcttc tttcggaaag atccatcatc tcgcggagga gtttctctat
     8521 gttgtcatca aagcttttac tctcgcctgc ataccttgcc gagtataccc cgggtgcacc
     8581 gcccagggat gaaacctcaa ggcctgtgtc gtctgcgaag acgttcaggc ctgtcttttc
     8641 atgcacatac cttgccttga agagggcatt ttcctcaagt gtttcggctt cctcgggaat
     8701 atcttcggtg ataccaaggt cagcaagccc gagaatcctg aaatttttat caagaagatc
     8761 acttatctcc ctgattttat gcttattgtt ggttgcgaat acaagttcca ttttctaagg
     8821 gataaaaggc aaaattaatc attgcattgt gacgctgctt taccggtata ccaaaaccgg
     8881 aaaattatag tttatttttg tatagatcac cagattaaaa tcctctatgg ttatcagcca
     8941 tttccgcggg ttcatgactg cttgcgtctt cctttggacg ggagcgtttg cggctgtgtc
     9001 tttgccgatg tctggggctg tgcctttgcc ggtgtctggg gctggcccgg ggcaagggtc
     9061 cggcctgcag tccgcggaga ggttgaccgg cgtttatctc tatcagtcag ggcagatgat
     9121 tgacacagct acggctgtac ctgtcttgag ataccgtgac ggaattatat tcaccatggg
     9181 aaatgatact gcagttgagt actcctattt catggccgga cctgacagtg agtggactga
     9241 atggactgac agggcataca aggagtacac gagccttgat gcgggaaaat atatcttcat
     9301 gtaccgttac agggtaaaat acgcaggagt tggcacaggc gagggttcat tcagattcag
     9361 ggtcaggcct ccatggtatt tctcactgct ggcgctgatc atttatccgc tgttgctgct
     9421 tgccgggggt atcatgatgc ggagacggac gcacaggctc tacacggaga ggcagagcag
     9481 gcttgaacag ctgatagcgg agagaacaga agagcttcag catgaaaaag agaagtctga
     9541 cagccttctt gccaatatgc ttccgaaggg cacagccgag gagataatgt caaaggggaa
     9601 agcagacaag agaaaatata atttcgttac ggtgctgttt tcagatatcc agggatttac
     9661 gaagattgct gaagagatga accctgagac cctgatagat gagctcgatc gcttcttctt
     9721 tcatttcgat tctgtggcgg agaaataccg gatagaaaaa atcaaaacga ttggtgacgc
     9781 ctacatgtgt gccggaggca tcccggagag aaacaggaca aatccggtcg aggtggttct
     9841 ggcagcgctt gagatgcaga agtacatgca ggtaatgaag aacgatccgg cccggcctgc
     9901 tgcccggttc tgggatatca ggatagggat acataccgga acggtggtgg caggtgttgt
     9961 cgggcacaag aagatcacat atgacatctg gggtgacacc gtaaacactg caagcaggat
    10021 ggaatcgtcc ggggaggcag ggaaaataaa catctcgggc actacctacg aatttgtaaa
    10081 ggagtatttt acatgtgact accggggcag gatgccggtg aagtacaaag gcgaccttga
    10141 catgtatttt gttaccggga taaggcctga gttgcgggag gctgacggat caccgaacag
    10201 gaagtttctt gcaaagatcg agatgatacg cattcttgat gtggaagagc atgtcttcac
    10261 caggtactct gaaatagcat ccccggatct cttctttcac agcgctgatt atatacggag
    10321 cgtatcattg caggctgacc tgcttgccag ggcagagaat ctttctgacg aggattacat
    10381 tcatctgagg ctggcttcgg ttttcattta ctttggttat gcctttgatt acaatgatcc
    10441 tgaggctgct gctctgaaaa gagccggtga gatattgtca ttttacggtt ttgatccgca
    10501 cacacttgag gcagtaaggg agctgatcgc ctcggcctct tcaccggaac agaccagcat
    10561 ggccggccgt gtgctgcatg atgcagtgta tgattttacc ggaagagttg attttatttc
    10621 catggttgat aaattgtaca gggaggagaa agcctacggg aagactggag acccgaaagc
    10681 ctggttcagg gagctggctt ccaggatcgg agagaacgga ttcctgacag aaacagcacg
    10741 caggctgagg tctgtggctg aggatgagca actcagggct ctccgggaat ttgccggcgg
    10801 ccggctcaac aatacttaaa atcatttgga actttggttt taaaccgtac atttgaaaaa
    10861 tccaaaattt gagatcatgg taaatttaga ctggaataat cttccttttg gctacatcaa
    10921 aaccgatttc aacgtaaggt gccattataa aaatggccag tggggcaagc cagaggtatc
    10981 ggattcagag tacatcaaca tccatatagc cgcaacagga cttcactacg gccaggaggc
    11041 cttcgaagga atgaaggcat accgcggacg tgacggcaag gtgaggttgt tccgttggga
    11101 tgagaatgca aaaagactta tatcatcggc aaacggaatc catatggctc cggtaccgat
    11161 tgagcttttc agggaggctg tctttactgc cgtgagactc aatgagaaat ttgttccccc
    11221 ttacggttcg ggtgcttcac tctatgtcag acccgtacta tacggaagtg gcgccgaagt
    11281 aggtgtgaag ccggcatcgg aatatacctt cattgtcttt gtaacccccg tgggtccgta
    11341 tttcaagggt ggagtgaaac cggtgaacat gctcatatgc cgcgatgttg acagggcagc
    11401 cccgctgggc actggcaaca taaaggttgg cggcaattat gcagccagcc tgagggccat
    11461 agttaaagcc cgtgagggcg gctacggcaa tgcgctcttc ctcgatgcat tggaaaagaa
    11521 ataccttgat gagtgcggtc cggccaactt cttcggaata aagaacaata cctacgttac
    11581 acctaagtcg gtatcaatcc ttccttcaat taccaataag agccttattg ccattgctga
    11641 aagcatgggg ataaagaccg aacggcgcag gattcccgtt gaagagcttg cagagtttga
    11701 ggagaccgga gcatgcggca ccgccgccgt tatcagtccg atagctaaaa tatttgaccc
    11761 cgaaaccggg aaggtttatg agtattgcaa ggacggcaaa ccgggccctg tttcaatgaa
    11821 gctctttaac aagcttgtgg ggattcagta cggcgatgag cctgatgagt tcggctggat
    11881 gaccgaggtt taaatcctga ggtgatattc aggaatttta tctgttaagc ctaaatatca
    11941 aaaaattgca gggctgctga aggatttggc ggtttgtatg ctatttttga ggcaaatttt
    12001 ctgaagtggc aaaatcaacg ggagggatat ccaggtacag actcggcagt tcatatgcca
    12061 cactcgttgt aagtgtttcc ctggtgctct tcctgctggg catccttggc atggtgctga
    12121 tcaatgcccg tcagttgtca gactatttca gggagagtct ctcgtttacc atcatgctga
    12181 aggaagacgc acgtgaggct gacataagga tgttgcagaa ggagcttgat gccaaacctt
    12241 atgtgaagca gacggaatat gtctccaagg acgaagcagc agttaagctg cgtaacgagc
    12301 ttggcgagga tttccttgat ttcctcggtt acaatccact gatgccgaca attgatgttt
    12361 atctgcaggc caggtacacg catcctgaca gtgtgataaa gcttgaaaaa gtcatcgcgg
    12421 attatccggt ggttgatgag gtttactacc aggagtcgat ccttaacctt atcaatgaca
    12481 atgttcgcaa gatcagcgta ttcctgttgg tgatcagtct tttgttattt cttatagcgc
    12541 ttacaatcat aaacaacacg ataaggcttt cgatttattc gaaaaggttc ctgataaaca
    12601 cgatgcagct tattggagcc aacaggtcgt ttattcgcaa gcccttcctg ctgaagagtc
    12661 tctttttcgg cttcctggct gcactgatag ctatcgggct gatgatggga ctgatgtatc
    12721 tgatagagaa agagtttttt acgctcttca cttatgagaa tatcaacctt ttcctcctgc
    12781 tttgcctggt acttgcggca gcagggattc tgattaacgg aatatctaca ttctttgccg
    12841 taaacaggta tctcggcatg cacgaggata aattatatta ttagaaaatc atggtcgcaa
    12901 aaaaagaaga agaaaaaaag accggtttcg ccctgggaag ggaaaactac aagctgatgg
    12961 ccataggctt cgctgtaatt attgtcggtt tcatacttat ggccggaggc aggtctgatg
    13021 accccaaagt cttctcggat gatatattca gtttccggag agttacactg gctccgctga
    13081 tcgttctggc cggctttatt tttgagatct atgcaataat gaaaaggcca agggagtaaa
    13141 gtctttgttc ttcttaaatc aaataaccgg gaatgaattg gtatgaggcc ctgattttcg
    13201 ggctggtgca gggattgact gagtttttac cggtcagcag tgacggccat cttgagattg
    13261 ttaaattcct gttcggaggg gttaaggaga gttttctctt ttctgttgtc gtgcatggcg
    13321 ctaccgtgct gagtatactt gtggtgttct ggaaggatat tttaaaactg cttgcggggg
    13381 tattcagatt taaaatgaat gaagagactg tctacgtttt gaagctgata gtgtcaatga
    13441 taccggtggc aattgtaggg tttaccctga aagaccaggt cgaatcgctg ttcgtagccg
    13501 acatggatat caccgggtca tttctgcttg tcacggcact gtttctgctg gtggggcatt
    13561 tcgtgcctaa aaggaataag ccgatcagtt atggaggtgc atttgtgata gggattgcac
    13621 aggcactggc tactcttcca gggctttcca gatccggctc aacaatttct acaggtctga
    13681 tgcttggcaa cagcaaggac gagctggccc ggttctcatt cctgatggta attgttccta
    13741 ttttgggagc caatttcata gagcttataa ccaccgatgc tggaaccgtt aacggtgtct
    13801 ttttcccgct tgttattgcg tttgttgcag cgtttgtgtc aggatacgca gcctgccgct
    13861 ggatgataaa cattgtcagg aagggtaagc ttgggtggtt tgcactttac tgtgttttgg
    13921 caggtctaac attaataata tttgcctgaa ggtgctgaag aggtgttccg actttgaaga
    13981 tggagaggtg ctgctgtttc agaagccgct ttactggacc tcctttgacc tagtgaacaa
    14041 ggtgaagctg gccatccgtc atcactgcgg attgaagaaa ctgaaagtcg gacatgccgg
    14101 cactctcgat ccgctggcaa ccgggctgat gattctttgc accgggaagg ccacaaggag
    14161 agtggaggag ttcagggatc tcgacaagga atatattgcg gtggtcaggt tcggtcagac
    14221 aactccttct tttgacctgg aaagtgaggt ggatgcagag tacccttccg gtcatattac
    14281 ggaggagttt gtcagggaaa agctgagagg atttttaggg gagcaggacc aggagcctcc
    14341 gcttttttcg gccaagtttg ttgacgggaa gagagcctat gagctggcca ggaagaagat
    14401 cgagcacaag cttgacagtg tgaaggttgt tttccgtgag ctggagctgg tggagtacag
    14461 cggggttgat gctactgtaa ggattgtctg cagcaagggg acctatatca gggctttcgc
    14521 ccgtgatctt gggcaggcgc ttggcagcgg tggtcacctg aggtcgcttg ttcgcagcgc
    14581 cataggtgat tttcgccttg aaaaggcact ttctgtagaa gagttcaaat tttttcttga
    14641 aaatttgaaa catagtgaaa actcattcgt ataaatattc ttgtactaac gtattttgat
    14701 catgaaacta tctcaattca agtacacact accccaggag ttgattgctc tttacccctc
    14761 caaaaacagg gacgaatcgc ggctgatggt agtgcacagg gataccgggg agattgagca
    14821 taaggtgttt aaagacatcg tggagtattt cagggagcgt gatgtgcttg tattcaacaa
    14881 caccaaggtt tttccggcca ggctgtacgg gaacaaggag aagactgggg ctgagataga
    14941 ggtgttcctg cttcgcgagc tgaaccgtga gcagaggctt tgggatgtgc tggttgaccc
    15001 ggcaaggaag atcaggattg gcaacaagct ttatttcggg gaggatgacc tgctggtggc
    15061 ggaagtgata gacaatacca catcgagagg cagaaccctt cgttttctct ttgacggaac
    15121 atatgaggag ttcaagcaga cgctttattc gctcggcgag actccgcttc cgaagttcat
    15181 caacaggcct gttgagccgg aggataatga gcggtaccag accatttttg caaagcatga
    15241 aggagcggtt gctgcgccga cggccgggct tcatttcagc agggaactgc tgaagaggct
    15301 tgagatagtt ggcgttgagt ttgccgagat cacccttcat gtggggcttg gcaatttccg
    15361 taatgttgat gtggaggacc tgaccaagca caaggttgat tccgagagga taatcatctc
    15421 cgaggatgca tcggtgctga tcaacaaggc caaagagagg aagagcagga tctgcgcagt
    15481 aggaaccacc gtgatacgga cactggagag ttctgtttct acctcgggaa tggtaaaacc
    15541 gtttaacgga tggaccaaca agtttatctt tccgccctac gacttcaggg tgcccgatat
    15601 gcttgtgagc aatttccagc tcccgtattc cactctgctg atgatggttt ccgcttttgc
    15661 gggctacaat cttctttttg atgcatatcg tactgccgtc aaggaaaaat acaggttcgg
    15721 aacctacgga gatgccatgc tgattcttta gagacgaggc atcaaaattg ttgataaaca
    15781 tttgataatg tggataatta gtgccggcat cacttgccgg cacttctctt ttctctgaaa
    15841 ttcacgcatt aagaggctaa attcttctgc aatgaagtaa ccattttgac gtttgatgag
    15901 tataaatgac gtacagcagt taaagaagcc acgtaatcaa cctgtagaat gttaagttac
    15961 aagaaagcga tatttccatt gcttgtaggg atattcgctt tattatttga gttatcagtt
    16021 tcagcccagt gcggaccggg gaaga
//
LOCUS       DAOPXU010000062        16011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_3895_length_16011_cov_10.563612, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000062 DAOPXU010000000
VERSION     DAOPXU010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16011
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3895_length_16011_cov_10.563612"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(359..1171)
                     /locus_tag="PKK03_07615"
     CDS             complement(359..1171)
                     /locus_tag="PKK03_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNX84307.1"
                     /translation="MKRFRLYTMVLLWFSAAVLLQAQTLVPRPDPPRLVNDLAGVLSS
                     GEVKALEDKLVAFNDSTSNQIAILIVNDLQGYDRSEFAFRVANDWGIGQGDLNNGVLV
                     LVKPKTSSSDGRVFIATGSGIEGAIPDLATADIIDREMIPRFRENDYYGGLDAGTNVL
                     MSLAAGEYNYDSYAGGAPSGAIPGVVIFLIILFFIIIVSAGSSNNKHMKRSGSSNLPL
                     WLLMSMMGGGRSHSGSWGGFSGGGGGFGGGGGGGFGGFGGGGFGGGGAGGSW"
     gene            complement(1174..1626)
                     /locus_tag="PKK03_07620"
     CDS             complement(1174..1626)
                     /locus_tag="PKK03_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNX84308.1"
                     /translation="MKASTFFTPEQQEVIVRSIREAEHATSGEIRVHIETSCKVDVLD
                     EAAWLFRKVGMHKTADRNGVLIYLALKERNFAIIGDSGINSAVPPGFWDGIRDHMQER
                     FSQGLFTEGLSEGVLMAGEKLKEHFPRSKDDVNELPDSISFDTNETGV"
     gene            complement(1637..2236)
                     /locus_tag="PKK03_07625"
     CDS             complement(1637..2236)
                     /locus_tag="PKK03_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNX84309.1"
                     /translation="MKKSISGLVILVIIVVALLLLVSWGVGIYNKIVPMNEKVASEWS
                     NVETQYQRRADLIPNFVNTVKGAAAFEQETLTKVIEARAKASQVTVDPSNITPEQLQE
                     FQAAQSEVSSALQRLMVVVERYPELKATQNFRDLQVALEGTENRISQARGTFNDAARE
                     YNTFIKKFPNNFIASLTKFNDRPYFEAQEGTEVAPKVEF"
     gene            2392..3369
                     /locus_tag="PKK03_07630"
     CDS             2392..3369
                     /locus_tag="PKK03_07630"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNX84310.1"
                     /translation="MRTIAIVAGGNSSEYPVSVRSAKGVESALKGKFETYIINIKGTD
                     WYWEDPRGRVCRIDKNDFSLVLDDQRIRFDAAFIAIHGNPGENGLLQGYFDMIELPYT
                     SCDAFVSALTFNKQACKLYLNEYGIPMTGGLLIRKGQKYDAAGIVRQTGLPCFVKPNA
                     SGSSFGVTKVKETTEFNEALEKAFAEGHEVLVESFMKGTEVACGVVKTRNRKIVMPVT
                     EVVPKKEFFDYEAKYTEGLSDEITPARFSEELTNRIQELSSEIYDILGCRGIVRVDFI
                     VTHDGPQFLEINTIPGMTPESIIPRQAAVFGISQTDLYSMVIEDLFTMV"
     gene            complement(3496..6384)
                     /gene="gcvP"
                     /locus_tag="PKK03_07635"
     CDS             complement(3496..6384)
                     /gene="gcvP"
                     /locus_tag="PKK03_07635"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNX84311.1"
                     /translation="MTSDRFVNRHVGPRESDAAVMCKAIGVDSPEQLIEQTVPASIRL
                     DRPLNLPPAMSEFEYLAHIRDLGKRNRQFRSFIGQGYYGVAVPPVIIRNVLENPAWYT
                     SYTPYQAEISQGRLEALLNFQTMVAGLTAMEIANASLLDEATAAAEAMIMMFNARSRD
                     KVKTGADLFLVDNSVFRQTRDVLITRAAPLGIDIQFVDYKLIEADPRMFGVIVQYPAA
                     CGEVRDYRELVEKVHGAGALVGVGADLMSLTLLAPPGEWGADIVFGSNQRFGLPMSYG
                     GPHAGFFATRDEMKRNMPGRLIGVSVDVDGNQALRMALQTREQHIKRERATSNICTAQ
                     ALLATMSGMYAQYHGPEGLKRIARHIFSTACTLAGALKKLGYTIKHEQFFDTVRVQAV
                     TGVTSDMIQKIALSRGINLWYPCANCAVISTDEVTTLKEINDIVGIFAEAAGKKADEV
                     TSLCDCFCIDEKFHRKSAFLQERTFNSYHSETDMMRYIKMLERKDFSLANGMIPLGSC
                     TMKLNPAASMFAMSWPEFGNIHPFVPADQAEGYHKMMEELGEALKEITGFDAVSFQPN
                     SGAAGEYAGLMVIRQYHISRNEGHRDVALIPSSAHGTNPASAAMAGMKVVVVECDENG
                     NISVEDLKKKAVEHRDNLSSAMITYPSTHGVFEEAIVEMIKIVHENGGLVYMDGANMN
                     AQVGLTSPGFIGADVCHLNLHKTFAIPHGGGGPGMGPILCNTKLAEFLPAHPVVKTGG
                     SHGITAVAAAPFGSAHVLTISHAYVMMLGAEGLTKSTVFAILNANYISAKLSGMYKVL
                     YTGANGRCAHEMILDCRGFKDAGVDESDIAKRLMDYGFHAPTLSFPVHGTLMVEPTES
                     EPKAELDRFVDAMRSIWNEIEEIRMGEADREDNVLKNAPHTAAAVISDGWKHVYAREK
                     AAFPLAWVVENKFWPTVGRVDNGYGDRNLVCTCAPIDAYRFEALEGRQ"
     gene            complement(6470..7105)
                     /locus_tag="PKK03_07640"
     CDS             complement(6470..7105)
                     /locus_tag="PKK03_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX84312.1"
                     /translation="MNIYRAVFSPMQVNTYIITGNGKECVIIDCGCYVGAEEQKLEEM
                     LSARRLKPVMLLDTHCHLDHVFGNRFMLERYGLRPWFHEGDRHNYQNAPKQSLMFGLS
                     MEPQPDPEGYLSDGQVISAAGVTLTVIAVPGHSPGGIAFYSATDGVVFTGDALFAGSI
                     GRSDLPGGDHEQLMENIRERLFVLPPDTVAYPGHGPETTIREEAEHNPFFM"
     gene            complement(7110..7775)
                     /locus_tag="PKK03_07645"
     CDS             complement(7110..7775)
                     /locus_tag="PKK03_07645"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNX84313.1"
                     /translation="MMIEESFEAKGKRKRLIEELRSKGINDERVLGAMGKVPRHLFMD
                     DAFLRHAYQDKAFPIAAGQTISQPYTVAVQTVLLGVGKRDKVLEVGTGSGYQAAVLAE
                     MGVKVYTIERQRELYRKAQIMLGDLGYRIHFFLGDGYEGQPQYGPYDGIIITAATAEV
                     PDKLLKQLRIGGRLVVPRGERDSQVMTLYERRGEDDYEITTHGYFVFVPMLRGIANGN
                     DRD"
     gene            7832..9253
                     /locus_tag="PKK03_07650"
     CDS             7832..9253
                     /locus_tag="PKK03_07650"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNX84314.1"
                     /translation="MKKFARTIRLSYILADFTGSSVAWGLFYLFRKYFIETKLYGDYF
                     RMELGVKFYLGISLIPLFFLVVFAMAGFYSDPLRRSRLLESGKSLAVTLAAVVVLFFL
                     LILDDVIGHYSTYYRSFGVLFLLEFMLTYIPRLIITSVVSGKIHRREAGFRTLIVGSR
                     EKAARLISEIRSEKMPAGNLLTGFLTVSGDSKRQPDVDIRRLGGADEIIRVIEEHEID
                     EVILAMEEDEYGQVEEIVNTVCYRDVVVRAVPSMRGIISGHVEAGPIYATPLLRVSYP
                     EMPRWQYMIKLFLDYSLSALALIFLSPLMAVLALLIRISGKGPVIFRQERIGRYGKPF
                     MIYKFRSMEEDAEPDGPKLAVKGDTRITPLGRFMRKHRFDEIPNFINVLRGEMSLVGP
                     RPERSHYIEQIRVRAPWFNRVLAVKPGITCWGQVKLGYASDLDTMLERLTYDLLYLEN
                     ISLFLDLKIIFYTLGTIIKGKGL"
     gene            complement(9255..9938)
                     /locus_tag="PKK03_07655"
     CDS             complement(9255..9938)
                     /locus_tag="PKK03_07655"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84315.1"
                     /translation="MRTTLIIGLVSALLLFSISLHAQQECRVLLPGISGSYNGDCKKG
                     LAEGEGTASGTDKYTGSFRKGLPDGEGTYTWATGAVYAGHWKKGMRDGYGTFTFPVNE
                     KDSIQAGYWSEDAYVGKEQLAPYVIQHRIGVTRASFLKQGKGESYVSFKFARSGNTSY
                     NVEDLIMQGSSGEESITTAFTGFQHTSFPFEGKIQFKAPNLLESGMYNYEMRFVINHP
                     GDWLITVYY"
     gene            complement(10048..11526)
                     /locus_tag="PKK03_07660"
     CDS             complement(10048..11526)
                     /locus_tag="PKK03_07660"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNX84316.1"
                     /translation="MKKIKMIGVIFISLLVLGSCTKGWEEMNIDPNNPTDVPAANIMT
                     NVERRLTNLLFDMWFTGNNTSSYANHIAKIQYIDENRYAERDGIIGYWDTFMTYVVEL
                     NKIIEKAQADENPAMEGAAMVIRAQIFHIMTDTWEAIPYTEGAKGEEGVFQPTYDSQE
                     DVYAALLADLKAANTKLAAGGSVDGDVLNDGDIMLWRKYANSLRMRMAIRMSNIDPAG
                     AKAVLTEILGDATTYPILASNDDVIGFEWVGVSPYFEPYYADLIEPRDDHGMCKTFID
                     ALLATADPRIGEFAHPAADDGIYRGLPAGITEGDEGFELGTISRIGTRYRDTPNGTNH
                     YFRYAEVEFIKAEAYARADLLNNPTAAKTAYEAGITASCAEHGVSAGNITTYMANTNV
                     AWDGAGSWGYTNVQKIAYQKWVSLFKQGQEAWAETRRTDIPLLSAAPGSIFSGHNRPP
                     FRWPYPTSEYTLNETVVKSFDTAIKDRFWGDKMWWDTRTGVN"
     gene            complement(11550..>12422)
                     /locus_tag="PKK03_07665"
     CDS             complement(11550..>12422)
                     /locus_tag="PKK03_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84317.1"
                     /translation="EIESNGVELSAYGEIVKTKDFSWMATVNWSKQKTVVNKLAEGVT
                     SLVLNSSWSPTTVEARPGEPFGQIYGIAYQRDEFGNKLLDGGYEIPTDAPVVCGNTLP
                     DWLGSINNTFRYKQFTGNFMIDAKWGGDIFTVTKWFGDYSGITETTVANGIREDGLVA
                     EGMIAEWIEDNAETGAGHWESTGVENDVRVSSQAYFGNYWGFTEPGVVDGSYVKLREI
                     NLGYDLRFRNKMVSNLNVGLYARNVAVLYLHPSNDIRIDPETGYGTGTDALGLEQYQL
                     PPVRSMGFKVRVDF"
     assembly_gap    12423..12522
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12523..14666)
                     /locus_tag="PKK03_07670"
     CDS             complement(<12523..14666)
                     /locus_tag="PKK03_07670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX84318.1"
                     /translation="MNRLRLLISLFVLTGFTALMAQTVTITGTVTSADDGMPIPSVSV
                     TVKGTTLGMLTDAAGRYSIAVPASATSLTFTFVGMKTITENISGRTTIDVVMQTDMLG
                     LDEVVVTALGITREKKALGYAVQEVSGSELANAEETNIVNALQGKAAGVQITRASGAV
                     GSSAQILIRGIKSFGDNQPLWVVDGTPISNSSSGATQWSGVDFGNNASDIDPEIIESI
                     SILKGANAAALYGSRARNGVVLVTTKRGKAGQIKVSLSNSTSFDNVAWLPAYQNEYGQ
                     GVNGEEYIYDIRSNTAGSPYFGMTYQDYAHAYSFRYVNGMGGGRGDGTDESWGPRLDA
                     GLKLNQFFAPADPSLSLAENLANAAPWVSHPNNVRDFFQTGITSTSSVSMEGGSDNMT
                     SRMTYTNVMEKGTIPNTDQKRNQINLSTVMNLTSKLKVDVALNYSKTGNDNLPGQGYD
                     VQNIMQSIGGWFGRQVDMKLLEENWDTFFDNGMPYNWNSNYHNNPYWTVNKNTTARNR
                     ERIFGNATATYQLTDWLNIVGRYGMDYYNEFRKHVTYEGSNDAPYGTGGYFWQNQRDN
                     RETNADVFLNFQKDFSALQVSGNIGASYRNVLYNYGSISADMLTVPNFFDVSNVQGSA
                     GTDMYRSEYEQNSVFGALNLGYASQVYLDFTIRNDWSSTLPKEEWSYLYPSVSLGWIF
                     TETFGLTESAFSFGKLRASWAKVGSDTSPYSLS"
     gene            14962..15618
                     /gene="elbB"
                     /locus_tag="PKK03_07675"
     CDS             14962..15618
                     /gene="elbB"
                     /locus_tag="PKK03_07675"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HNX84319.1"
                     /translation="MKAAVVLAGCGVRDGAEIHEAVMTMYAIDKLGGTFHIFAPNVAQ
                     HHVINHITGEEMKESRNVLTEAARIARGKISPLTDYRAEDFDTLIFPGGFGVAKNLCT
                     YAFDGPDCHVDRVVEKTIRSTHSAGKPIGALCISPVLIAKVLGDVTVTIGNDRQTAAD
                     IGKLGGKHENRQHGEVTIDRKNLIVTAPCYMINATISEIARDAEAVVKALIELTGKKA
                     "
     gene            complement(15654..>16011)
                     /locus_tag="PKK03_07680"
     CDS             complement(15654..>16011)
                     /locus_tag="PKK03_07680"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HNX84320.1"
                     /translation="ALIVRDEFGRINRLLETLPTDRAKRDFLQSYEKDLLAQYEGDIR
                     KLTFTQGKILIKLIDRETQNTSYDLIRQYRGKFSATFWQGVARIFGANLKSNYDPNGD
                     DYLIEYIVREIEAGRL"
BASE COUNT         3800 a         4233 c         3868 g         4010 t
ORIGIN      
        1 accaggaatc tggcattgag agttatatcg ctgtgcggac aaatgccggt gacaattcca
       61 aataatatcg taggccgaca gttaatcagg gcttcatgct ctgtaggatc caactatcgg
      121 gtagtcagcc gggcaaaatc gacagctgat ttcataaata aactccatat agttgaagag
      181 gaggctgatg agtccatatt ctggttagaa ttgctgcagg aaattgaacc atcatacaaa
      241 gactcaatca tgtcattaat ttcggaaatg aatgaaatcc tttcaatagt tgttgctgca
      301 agaaagactg ccaaaaacaa attttcagac aaaaatccta aatcgcaaat cgcaaatcct
      361 accagcttcc cccggctccg ccgccgccaa aaccgccgcc gccaaagccg ccgaaaccgc
      421 ctccgccgcc acccccgaag cctcctccgc ctccggagaa tccgccccag cttccgctgt
      481 gagacctgcc tccgcccatc atgctcataa gaagccacaa cggcaggttt gaagatccgc
      541 tgcgtttcat gtgcttgttg ttggagctgc cagcggagac aatgattatg aaaaagagaa
      601 ttatcaggaa gataactacg ccgggtatgg ctcccgaagg agcgccgccg gcataactgt
      661 catagttgta ctcgcctgcg gcaagtgaca tcagcacgtt cgtgccggca tcaagcccgc
      721 cgtaataatc attctccctg aacctcggaa tcatctcccg gtcaataatg tctgcagttg
      781 ccaggtccgg gattgcccct tctataccgg aaccggttgc gataaacacc cgaccgtcag
      841 aggatgaggt cttgggtttt accagcacaa gcaccccgtt gttgagatca ccctgcccga
      901 ttccccagtc gttggccacc ctgaacgcaa actcggaacg gtcatatccc tggaggtcat
      961 tcacaataag aatggcaatc tggttcgatg tggagtcatt gaatgcaaca agcttgtctt
     1021 caagagcctt cacttccccg gaagaaagaa cgccagccag gtcgttgacc agtctcggcg
     1081 gatccggccg tggtaccagt gtctgtgcct ggagcaggac tgctgccgaa aaccacaaca
     1141 gcaccattgt atataatcgg aatctcttca ttgtcatact cccgtttcat ttgtgtcgaa
     1201 cgagatgctg tcaggcagct cattgacatc gtcctttgat ctcgggaaat gctcctttag
     1261 cttctcccct gccatcagga ctccctctga gagtccttca gtgaagaggc cctggctgaa
     1321 ccgctcctgc atatggtccc ggataccgtc ccagaacccg ggcggcacag ccgaattgat
     1381 ccctgaatcc ccgataatgg caaaattgcg ctccttcagg gcgaggtaga tgagtactcc
     1441 gttacggtct gcggtcttgt gcatcccaac cttcctgaag agccatgccg cctcgtccag
     1501 aacatcaacc ttgcacgaag tctcaatatg cacacgtatc tccccggagg ttgcatgttc
     1561 ggcctcacgg atggatctga cgataacttc ctgctgttca ggtgtaaaga aagtcgatgc
     1621 cttcatcccc ggtatgtcag aattcaacct ttggcgctac ctcggtaccc tcctgagcct
     1681 caaaataagg cctgtcatta aacttcgtca gcgatgctat gaagttgttc gggaatttct
     1741 taatgaaggt gttgtactcg cgggctgcat cgttgaaggt accccttgcc tgcgatatgc
     1801 ggttctcggt tccttccagc gccacctgca ggtcacggaa gttctgggtg gccttcagtt
     1861 ccggatacct ctccacaaca accatcagtc tctgaagggc ggagcttact tcgctctgcg
     1921 cggcctgaaa ctcctgtaac tgctcaggtg tgatgtttga cgggtcaaca gtgacctggg
     1981 aagcctttgc ccttgcttcg atcaccttgg taagcgtctc ctgctcgaaa gcggcagcac
     2041 ccttgaccgt attcacgaag ttggggatca ggtcagccct ccgctgatac tgcgtttcca
     2101 cattgctcca ttcagaggca accttctcat tcatcggaac tatcttgttg taaatgccta
     2161 ctccccagga aacaagaaga agcagggcaa cgacaataat cacaaggatt acaagtccgc
     2221 taatactctt tttcattttt ctgtcagttt gattatatct caaaaataaa taaaatatcg
     2281 ctggtaaaga cttgccggaa atcttctttg atgcagctcc ggaatggttt tttatgttct
     2341 tgtgccggca cgaaaatatt tgtagtaatt ttggacaaaa cttaccgaat catgagaacc
     2401 attgccattg tcgccggagg gaattcatca gaataccctg tctcggtacg cagcgctaag
     2461 ggtgttgaat cagctcttaa ggggaaattc gaaacataca ttatcaacat caaaggtacc
     2521 gactggtact gggaagatcc caggggacgc gtctgcagga tagacaagaa tgatttctca
     2581 ctggttcttg atgatcagcg catccgtttc gatgctgcct ttatcgccat ccatggcaac
     2641 cccggtgaaa acggactgct ccaggggtat tttgacatga tcgaattgcc ctacacgagc
     2701 tgcgatgcat tcgtctcggc gctcaccttc aacaagcagg cgtgcaagct ctacctgaat
     2761 gagtacggta ttccgatgac cggcggcctt ctcataagga aaggacagaa gtatgatgcg
     2821 gccggcattg tcaggcagac tgggcttccc tgttttgtca aacccaacgc aagcggatcg
     2881 agcttcgggg ttacaaaggt gaaggagact actgaattta atgaggctct ggagaaggca
     2941 ttcgccgaag gccacgaagt gctggtggag tcatttatga aaggaaccga ggtcgcctgc
     3001 ggggttgtca agacaagaaa caggaagatc gttatgcctg tgacagaggt ggttcccaaa
     3061 aaggagttct ttgattatga ggcgaaatat accgaaggat tgtctgatga gatcaccccg
     3121 gcacgttttt ccgaagagct caccaaccgt atccaggagc tgtcatccga gatttatgac
     3181 atcctcgggt gccgtggaat cgtcagggtc gatttcattg tcactcacga tggtccgcag
     3241 ttcctggaga taaacaccat cccgggcatg acccctgaga gcataatccc ccgccaggca
     3301 gcagtattcg gaatcagcca gacagatctt tattcaatgg tgatagaaga tctctttaca
     3361 atggtttaac caattcggtc agaaccggct tcgcaaattc gcaattttga gcctgcgaca
     3421 taggagcagg atcaataccg tatccgccgg ctggcggatc gggacgcaaa tcgcaaatcg
     3481 caaatcgcaa tttctctatt gcctgccctc cagggcttcg aacctgtagg cgtcaatggg
     3541 agcacaggtg cagaccaggt tgcggtcacc gtaaccgttg tccaccctgc caaccgtcgg
     3601 ccagaacttg ttctcgacta cccatgcgag cgggaatgcc gccttctccc gggcataaac
     3661 atgcttccac ccgtcagaga tgacagccgc agcagtgtgt ggagcgttct ttaacacatt
     3721 atcctcacgg tctgcctcgc ccatccggat ctcctcaatc tcattccaga tagaccgcat
     3781 ggcatcgaca aacctgtcaa gctcagcctt cggctccgac tcggtaggct caaccatcag
     3841 tgttccgtga accgggaaag atagcgtagg tgcatggaac ccgtagtcca tcagcctctt
     3901 ggctatgtct gactcatcca ctcccgcgtc tttgaatccc ctgcagtcaa gtatcatctc
     3961 atgggcacag cggccgttgg cgccggtgta aagcaccttg tacatgccac ttagctttgc
     4021 agagatatag ttggcattaa gtattgcaaa gactgttgac tttgtcagac cttcagcgcc
     4081 cagcatcatc acgtaggcgt gactgattgt caggacatga gcgctgccga atggcgctgc
     4141 ggcaacagcg gtgatcccgt gacttccgcc cgtttttacc accgggtgtg ccggcaggaa
     4201 ctcagccagc ttcgtgttgc agaggatggg gcccattccc ggcccgccgc caccatgagg
     4261 tattgcaaag gtcttgtgca ggttgaggtg acacacgtcg gccccgataa aaccgggact
     4321 tgtgagtccc acctgggcgt tcatgttggc gccgtccata taaaccagtc cgccattctc
     4381 atgcactatc tttatcatct ccacgatagc ctcctcaaaa acgccgtgcg tggaaggata
     4441 tgtaatcatt gctgagctga ggttgtcacg atgctcaacc gccttcttct tcaggtcctc
     4501 gacgcttatg ttgccgttct cgtcgcactc aactacaaca accttcatgc cggccatggc
     4561 ggcgctggca gggttggtgc cgtgagctga cgaagggatc agcgccacat cgcggtgacc
     4621 ttcattgcgg ctgatatgat actgcctgat aaccatcaga ccggcgtatt cacctgccgc
     4681 acccgagttg ggctggaagg acacagcatc aaagccggtg atctccttca gcgcttcgcc
     4741 cagttcttcc atcatcttgt ggtaaccctc cgcctggtcg gcaggaacaa acgggtgtat
     4801 gttaccaaac tccggccagc tcatcgcgaa catcgaggca gcagggttga gtttcattgt
     4861 gcacgatccg agcggtatca tgccgttggc cagtgaaaag tccttgcgct caagcatctt
     4921 gatatagcgc atcatgtcag tctcgctgtg atagctgttg aaggtacgct cctgcaggaa
     4981 cgccgacttg cggtggaatt tttcgtcaat gcagaaacag tcgcagagcg aagtaacctc
     5041 gtcggccttc ttccctgccg cctcggcgaa gatcccgact atatcattga tctccttcag
     5101 tgtcgtcacc tcatcggtgc ttatcaccgc gcagttggca cacggatacc agaggttgat
     5161 accccggctg agggcgatct tctgaatcat gtctgaagta acaccggtta ctgcctgcac
     5221 ccttactgta tcaaaaaact gctcatgctt tattgtgtat cccagtttct tcagagctcc
     5281 ggccagggta catgccgttg agaagatatg gcgggcaatt ctcttcagtc cttcaggacc
     5341 gtgatactgc gcatacattc ctgacatcgt tgccagcagg gcctgcgccg tgcagatgtt
     5401 cgaggtggcc cgctcacgct tgatatgctg ctcgcgtgtc tgcagcgcca tgcggagcgc
     5461 ctggtttcca tccacgtcaa ccgagacgcc tatcaggcgg cccggcatgt tgcgtttcat
     5521 ctcgtccctg gtggcgaaaa atcccgcgtg cggaccgccg tagctcatgg ggaggccaaa
     5581 cctctggttg gaaccgaaga cgatgtcagc cccccattct ccgggaggtg ccagaagggt
     5641 gagactcatc aggtcagcgc ctacgcccac gagggctccg gcaccatgaa ccttctcaac
     5701 cagctcgcgg tagtcacgta cctcgccgca ggcggcggga tactgaacaa tcacaccaaa
     5761 catccgcgga tctgcttcaa tgagcttata atcaacaaac tgaatgtcta tacccagagg
     5821 agctgcacgg gtgatgagca catcacgtgt ctgcctgaag acagaattgt caacaaggaa
     5881 aaggtctgct cctgtcttca ccttgtcgcg cgatcgggca ttgaacatca tgatcatcgc
     5941 ttcggctgct gccgtggcct catcgagcag tgacgcgttg gcaatttcca tggccgtgag
     6001 gccggccacc atcgtctgga agttcagcag cgcctcaagc ctcccctgtg agatctcggc
     6061 ctgataggga gtatatgagg tataccatgc gggattctca agcacattac ggatgattac
     6121 gggaggaaca gccaccccgt agtaaccctg tccgataaat gaacggaact gcctgttgcg
     6181 cttgccgagg tccctgatgt gggcaaggta ttcgaactca ctcatcgccg gaggcagatt
     6241 cagaggtctg tcaagcctga tcgatgcagg cactgtctgt tcaataagct gctcgggtga
     6301 atcaacgccg atggccttgc acatcacggc tgcatcactc tcacgcggcc cgacgtggcg
     6361 gttaacaaaa cgatcagatg tcatattata tacggttggt ttttcgtctg aaaggaacga
     6421 agcgaaaata gcataaagcg aagaacaaaa aaaagcatat tcatcatgtt tacatgaaaa
     6481 agggattgtg ttcagcctct tccctgatgg ttgtttcagg accgtgaccg ggataggcca
     6541 ccgtatcagg gggcaggaca aaaagcctct cacgtatatt ttccatcagt tgttcatgat
     6601 ctccgcccgg caggtctgac ctgccgatgc ttcctgcaaa gagtgcatca ccggtaaata
     6661 cgacaccgtc agttgccgaa taaaatgcaa tgcctccggg tgaatggcct ggtacggcaa
     6721 taaccgtcag cgtcaccccg gcggcgctga tcacctgccc gtcagacagg tatccctcag
     6781 ggtcaggctg cggttccatg gagagcccga acatgagtga ttgtttgggc gcattctggt
     6841 agttgtgcct gtcgccctca tgaaaccacg gccttagccc gtatctctca agcatgaacc
     6901 tgttgccgaa gacatggtcg agatggcagt gcgtgtcaag cagcatcacc ggtttcagtc
     6961 tcctggcaga cagcatctct tcaagcttct gctcttcggc accgacatag cagccgcaat
     7021 caatgatgac acactccttc ccgttgccgg taatgatgta agtgttaacc tgcatgggcg
     7081 agaatactgc cctgtatata ttcataatgt cagtctctgt cgtttccgtt ggcaattccc
     7141 ctcagcatgg gtacaaaaac aaaatatccg tgtgttgtga tctcgtagtc atcttctccc
     7201 cggcgctcgt aaagcgtcat cacctgcgaa tcgcgctctc ccctgggcac caccagcctt
     7261 cctccgatcc gcagctgttt cagcagctta tcgggcacct cggcggtggc tgccgtgatg
     7321 attattccgt catacggtcc gtactgcggt tgtccctcat aaccgtcacc caggaaaaag
     7381 tgtatccggt agcccaggtc tccgagcatg atctgcgctt tgcgatacag ctccctctgc
     7441 ctttcaattg tataaacctt cactcccatc tctgcaagca ctgccgcctg gtaacctgat
     7501 ccggtcccta cctccagcac cttatccctt ttcccaaccc cgaggagcac agtctgaacg
     7561 gccacggtat aaggctgtga gatggtctgc cctgcagcta tcgggaaggc cttgtcctga
     7621 taggcatgcc tcaggaatgc atcatccata aaaagatgcc ggggcacctt ccccatggcc
     7681 ccaagaaccc tttcatcatt gatccccttg ctcctgagtt cctcaatcag cctttttctt
     7741 ttacctttcg cctcgaagga ttcctcaatc atcatctcat gaatttggtc taaaaataac
     7801 agatatcttt gcatatctcc cgtacagtgt gatgaaaaaa ttcgcccgca ccatcaggtt
     7861 atcatatatt ttagctgatt tcaccggctc gtcggtggcg tgggggctct tctacctgtt
     7921 ccggaaatac ttcattgaaa cgaagttata tggcgattac ttccggatgg aactgggggt
     7981 caagttttat cttggaataa gcctcatccc gctgttcttc ctggttgtgt tcgccatggc
     8041 gggattctat tcagacccgc ttcgccgttc gcggctgctt gaatccggaa aatcacttgc
     8101 agtgacactg gctgcagtag tggtcctgtt cttcctcctt atcctggatg acgtcatagg
     8161 ccattactca acatattaca ggtctttcgg cgtactcttc ctccttgagt ttatgctgac
     8221 atatatcccg aggctgataa tcacctcggt agtttcaggc aaaatccacc ggcgcgaggc
     8281 aggtttcagg acgctcatcg taggaagcag agaaaaggct gccaggctta taagtgagat
     8341 aaggagtgag aagatgcccg ccggcaatct tctgaccgga ttcctgacag tcagcggtga
     8401 ctcaaaacgg cagcctgacg ttgacatcag gcggctaggg ggtgctgatg aaatcatacg
     8461 cgtcattgag gagcatgaga ttgacgaggt gatactcgcc atggaagagg atgaatacgg
     8521 ccaggttgag gagattgtca acactgtctg ttaccgtgat gtggttgtca gggctgtacc
     8581 atccatgcgg ggaattattt caggtcatgt tgaggcgggg cctatctatg ccacacctct
     8641 gctgagagta tcctacccgg agatgccacg ttggcagtat atgataaaac tgtttctgga
     8701 ctattcattg tcagcgcttg cgcttatttt tctgtctccg ttgatggctg tactggccct
     8761 ccttatcagg atttccggaa agggtccggt catcttcaga caggagcgaa ttggcaggta
     8821 tggcaaaccc ttcatgatct acaagttcag gtcaatggag gaggacgccg agcctgacgg
     8881 tccgaagctg gcggtcaaag gtgacacccg gatcaccccc ctggggaggt tcatgagaaa
     8941 gcaccgcttt gatgagatac ccaatttcat caatgtactg aggggtgaga tgtcacttgt
     9001 agggccgagg cccgagcgga gtcattatat tgagcagatc agggtaagag cgccgtggtt
     9061 caaccgggtt cttgccgtga aaccgggcat aacctgctgg ggtcaggtga agcttggata
     9121 tgcttccgac ctggatacaa tgcttgaaag actgacctac gacctgctct acctggaaaa
     9181 tatctcgctg ttccttgatc ttaagatcat cttctacaca ctgggaacga taatcaaggg
     9241 aaaaggcctg taaatcagta gtagacagtt atcagccaat cgcccggatg attgattaca
     9301 aaacgcatct cataattgta catgccagat tcaagcaagt tgggagcctt gaactggatt
     9361 ttaccctcaa acgggaaaga tgtatgctga aatcccgtga acgcagtggt aatgctctct
     9421 tcacccgagc tgccctgcat tatcaggtct tcaacattat atgatgtatt gcctgagcgc
     9481 gcaaacttga acgaaacata actttctcct ttcccctgtt tgaggaatga tgcccttgta
     9541 acacctatcc tgtgttgaat gacataggga gccagctgct ctttgccaac atatgcatcc
     9601 tcactccagt atccggcctg gattgagtcc ttttcattaa ccgggaaagt aaatgttccg
     9661 tatccatccc tcattccttt cttccagtgc ccggcataga cggctcccgt tgcccaggta
     9721 tatgtacctt caccgtccgg cagccctttc ctgaagcttc cggtgtattt gtcagtgccg
     9781 gaagcagttc cttcaccttc cgccagacct ttcttacaat caccgttata acttcctgaa
     9841 atacctggca aaaggacccg gcattcctgc tgtgcatgaa gggaaattga gaagagcagt
     9901 aatgccgata cgagacctat aattaatgtt gttctcatgc caattaattt tttgtttgaa
     9961 tactgcatac atcaaatata gtcaaaacaa attaaaagaa gggagttctt tgtctgaact
    10021 ccctctttgc tatcggatta agatctgtta attcacacct gtgcgtgtgt cccaccacat
    10081 cttgtcaccc cagaacctgt ccttaattgc agtgtcaaaa gattttacca ctgtttcgtt
    10141 aagggtgtat tctgaagtcg ggtagggcca gcggaaaggc ggcctgttgt gtcctgagaa
    10201 gattgagccg ggagctgcgg aaaggagcgg gatgtcagtc ctgcgtgttt cagcccatgc
    10261 ttcctggccc tgtttgaaga gggagaccca cttctggtat gcaatcttct gaacgttggt
    10321 ataaccccat gaaccggctc cgtcccatgc aacgttggta ttagccatgt aggtggttat
    10381 gttcccggct gagactccgt gttcagcgca tgatgctgtg atgcctgctt cataggcagt
    10441 ctttgcggct gtgggattat tgagaaggtc agcgcgggca taggcttcag ccttgatgaa
    10501 ttccacctcg gcataacgga aataatggtt tgtgccgttg ggggtgtcac ggtaccttgt
    10561 gcctatcctg cttattgtac caagttcgaa gccctcatca ccttcggtaa tgcctgccgg
    10621 cagtcccctg tatattccgt catctgcagc cggatgggca aactcgccta tcctcgggtc
    10681 agcggtggca agaagtgcat caataaatgt tttgcacatt ccatggtcat ccctgggttc
    10741 aatcaggtca gcgtagtacg gttcaaagta ggggcttact ccaacccatt caaagccaat
    10801 gacgtcatca tttgaagcaa ggattggata tgttgtggca tcacccagta tctctgtcag
    10861 tacagccttg gctccggcgg gatcgatgtt tgacatgcgg atagccattc tcatgcggag
    10921 agagtttgca tactttctcc acagcattat gtcaccatcg ttgagaacgt cgccgtcaac
    10981 gctgccaccg gcagcaagct tggtattggc agccttaagg tcggccagca gtgcagcata
    11041 gacatcttcc tgcgaatcat aggtgggctg gaagacacct tcttcgccct tggcaccctc
    11101 agtgtaggga attgcttccc aggtgtcagt cataatgtgg aatatctgtg ccctgataac
    11161 cattgcagca ccctccattg ccggattttc atcggcctgt gccttttcaa tgattttgtt
    11221 cagttcaacc acgtaggtca taaacgtgtc ccagtatccg ataattccgt cacgttcagc
    11281 atacctgttc tcgtcgatat actgaatctt ggcgatatgg tttgcgtagg acgaggtgtt
    11341 gttccctgta aaccacatgt caaagagcag gtttgtcaat cgcctctcaa cgttggtcat
    11401 gatatttgca gccggaacat cagtaggatt attcgggtca atgttcattt cttcccaccc
    11461 cttggtgcag cttccaagta cgaggagaga gatgaagatt actcctatca tttttatctt
    11521 tttcataatc aattcttttt aggatttaat taaaagtcca ctctcacctt aaagcccata
    11581 ctgcggacag gaggcagctg atactgctca aggccgagag cgtcggttcc ggttccataa
    11641 ccagtctccg gatcaatcct gatgtcattg ctcggatgca ggtaaagcac cgcaacgttc
    11701 ctggcataaa gcccgacatt aagattgctt accatcttgt tcctgaacct caggtcatat
    11761 ccaaggttta tttcgcggag cttaacatat gaaccatcta caacccccgg ttcggtaaat
    11821 ccccagtagt tgccaaaata tgcctgggag gaaaccctga catcattttc aactccggtt
    11881 gattcccagt gtccggcgcc tgtttcagcg ttatcctcaa tccattcagc tatcattccc
    11941 tcggctacca atccatcctc acgaatgcca ttggcaacag tcgtctcggt tatccctgaa
    12001 tagtcgccga accatttggt taccgtgaag atgtctccac cccacttggc atcaatcatg
    12061 aaattaccgg taaattgctt gtaacggaaa gtattgttga tgcttccaag ccagtcggga
    12121 agagtgtttc cgcatactac cggagcatct gtcggaatct cgtatcctcc gtcaaggagt
    12181 ttgttgccaa actcatcacg ctgataggcg atgccatata tttgtccgaa cggttcacca
    12241 ggtctggcct caactgtggt cggagaccat gaactgttaa gcaccaggct tgtaaccccc
    12301 tcggcaagct tgttgacgac agttttctgc tttgaccagt taaccgttgc catccaggag
    12361 aaatccttcg tctttactat ttccccgtag gctgataatt caacaccgtt tgattcgatc
    12421 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngaaaggct gtaagggctg
    12541 gtatcgctgc ccacctttgc ccagctggca cgaagcttgc caaacgagaa ggctgattca
    12601 gtaagaccga atgtctcggt aaatatccat cccagtgaaa ctgaaggata aaggtagctc
    12661 cattcctcct tcgggagggt tgaactccag tcattcctga ttgtgaagtc aaggtacacc
    12721 tggctggcat agccaaggtt caatgctccg aatacactgt tctgctcata ctcggacctg
    12781 tacatatctg ttccggcgct tccctgaacg tttgagacat cgaagaagtt gggaacagtc
    12841 agcatgtcag cactgattga tccgtagtta taaagcacgt tcctgtatga ggcacctatg
    12901 tttccggata cctggagggc tgagaagtct ttctggaaat taaggaagac atcagcgttg
    12961 gtctcacggt tatcacgctg gttctgccag aagtaaccac cggttccgta aggagcgtca
    13021 ttggaacctt catatgtaac atgcttacgg aactcattgt aatagtccat accgtaacgg
    13081 ccaacgatat tcagccagtc tgtcagctgg taagtggctg ttgcattgcc gaagatcctc
    13141 tccctgttac gggctgtggt gttcttgttg actgtccagt acgggttgtt atgatagttg
    13201 ctgttccagt tgtagggcat cccgttgtca aagaaggtat cccagttttc ctccagaagc
    13261 ttcatatcaa cctgacgtcc gaaccatccg ccgatagact gcatgatgtt ctgtacgtca
    13321 taaccctgtc ccgggaggtt gtcattgccg gttttggagt agttgagggc aacgtcaacc
    13381 ttcagtttcg aggtaagatt catcacggtg ctgaggttga tctggttcct cttctggtca
    13441 gtattcggaa tggttccttt ctccatcacg tttgtgtagg tcatgcggga ggtcatatta
    13501 tcgctacccc cttccatgga aacacttgac gttgatgtta tccctgtctg gaaaaagtcc
    13561 cgaacgttat tcggatgtga aacccatggt gcagcatttg ccaggttctc tgcaagagag
    13621 agagacgggt cagcaggagc aaagaactga ttcagtttca ggcctgcatc aagcctcggg
    13681 ccccatgatt catcagtacc atcgccacgt ccgcctccca taccattcac atacctgaat
    13741 gaatatgcgt gagcataatc ctggtaagtc attccgaagt aaggtgaacc tgcggtatta
    13801 ctacgaatat cataaatata ttcttctccg ttcacaccct gaccatactc gttctggtag
    13861 gcaggaagcc atgctacgtt atcaaaggag gtgctgtttg acaggctcac cttgatctgg
    13921 ccggccttgc ccctcttggt ggtaacaagg acaaccccgt tacgtgcacg gctgccgtaa
    13981 agagccgcag cgtttgcgcc cttaagaatc gagatcgatt cgatgatctc cgggtcaatg
    14041 tctgaagcgt tgttcccgaa gtcaacacct gaccactggg tggcaccgct tgatgagttt
    14101 gaaatcggag taccgtcaac cacccacaga ggctggttgt caccgaatga tttgataccc
    14161 ctgataagaa tctgtgctga agatccgaca gcaccggaag ccctggtgat ctgcacaccg
    14221 gcagcctttc cctgaagggc attcacaatg tttgtctcct cggcattggc cagctcggag
    14281 ccagacactt cctgcactgc gtatcctaga gctttcttct ccctggtgat acccagcgcc
    14341 gtcacaacca cctcatccag accgagcatg tcagtctgca ttaccacatc aatagtggta
    14401 cggccactga tattttcagt gatggttttc atcccgacaa atgtgaaggt aagtgaagtt
    14461 gcacttgcgg gtacagcaat cgaataccgg ccggcagcat cagtcagcat gccgagcgtg
    14521 gtacctttga ctgttacgga tacgctggga atgggcatgc catcatcagc cgacgtaaca
    14581 gtaccagtaa tagtcaccgt ctgagccatc agggctgtga agcccgtcag cacaaacagc
    14641 gaaatcaaca gtcttagcct gttcatagat cagagtttaa gttagacaat caggtttcta
    14701 tttacctttt cctgcttgac acagactgca actaagccag aatatgtctc acaagagaac
    14761 tttaaaagta gatatatttt ttttctctga atatgattac ccgttttttt cacccggaga
    14821 cacattaaaa atcagtattt caggcaaaat gaggtgaatt tttcgatgtt tatacccggt
    14881 tgtgccggat acaagctcct gaaaatggaa aggatcaggg atttttctac ctttattacg
    14941 cttataaaac ataccatata tatgaaagca gctgtagtac ttgccgggtg cggcgtcagg
    15001 gacggtgccg agatacacga ggccgtgatg accatgtatg ccatagacaa acttggcggc
    15061 accttccata tatttgcgcc caatgtggca caacatcacg taatcaacca cataaccgga
    15121 gaggagatga aggagtcacg caacgtactc accgaggcgg cacgtatagc aagaggcaaa
    15181 atcagccccc tcaccgacta cagggccgaa gattttgaca ctctcatctt ccccggagga
    15241 ttcggcgtgg ccaaaaatct ctgcacctac gcctttgacg gccctgactg ccatgtcgac
    15301 agggtggtgg agaaaaccat acgctccact cacagtgccg gcaaaccgat aggggcgctg
    15361 tgcatctcac ccgtgcttat tgcaaaggtg cttggtgatg tgacggtgac aataggtaat
    15421 gaccggcaaa ccgcagctga cattggtaaa ctgggcggaa aacacgaaaa ccggcaacat
    15481 ggtgaggtga ccatagacag gaaaaacctc attgtaacag caccatgcta tatgatcaac
    15541 gctactatct cagagatagc ccgcgatgct gaagcagtgg taaaagccct gattgaactg
    15601 accggcaaaa aagcataacc cgggatcaga ttggactgac cggcaaaaga ccgtcataac
    15661 cgcccggcct caatctccct gacgatatat tctataagat agtcatcgcc atttgggtca
    15721 tagtttgact taaggttggc accaaagatg cgggccacac cctgccagaa ggtggcggaa
    15781 aactttccgc ggtactgcct tatgagatca tacgatgtat tctgagtctc cctgtcaatc
    15841 agcttgatga gtatctttcc ctgggtgaag gtcagttttc ttatatctcc ctcatactgc
    15901 gccagtaaat ccttctcata agactgcagg aaatcacgct ttgcacgatc agtcggaaga
    15961 gtctcaagaa gcctgtttat ccggccgaac tcatccctga ctatcagcgc a
//
LOCUS       DAOPXU010000063        15635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_4040_length_15635_cov_12.358793, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000063 DAOPXU010000000
VERSION     DAOPXU010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15635
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4040_length_15635_cov_12.358793"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PKK03_07685"
     CDS             <1..303
                     /locus_tag="PKK03_07685"
                     /inference="COORDINATES: protein motif:HMM:NF021381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNX84321.1"
                     /translation="LNGSLTRHCASPAGFVTTGKDQIRKKMKSEMPMIKYTCPKCGGR
                     KYRLSEIRVAHSLFTQLFDIQARKYSAMICEKCSYTEFYNVPVKKITSVFDFFTST"
     gene            complement(306..1010)
                     /locus_tag="PKK03_07690"
     CDS             complement(306..1010)
                     /locus_tag="PKK03_07690"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNX84322.1"
                     /translation="MQIFYCEDISGDKAVFSREESGHCLRVLRMRRGDTLTFTDGRGN
                     LYDGVIATDDPKGMSVTITSAKSDPLPGKYRLHVAISPLKNEDRLEWFVEKAVEIGVD
                     VITPVICSRSEKKRIRRERLEGIIISAMKQSGRCILPRLEEPETAEEFTRRPAKGKRI
                     IATCDSSFDRRGITEAFNRGDNVTILIGPEGDFTPEEVKAAISAGYVPVHIGTGRLRT
                     ETAGIAACCSVYLANI"
     gene            1073..1582
                     /locus_tag="PKK03_07695"
     CDS             1073..1582
                     /locus_tag="PKK03_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNX84323.1"
                     /translation="MRIGVISDTHGWIDPAVYEHFSGVDEIWHAGDAGNAGVIAELVA
                     FRPLRAVWGNIDGFDVRRETKEYLFFGAGSKNVLMIHIGGYPGRYAPRALELIRELRP
                     DIFVCGHSHIAKVIYDKTFGMLCINPGAAGRTGIHKVMTMLRFTIDGANVSDMEVIEF
                     GSRGRSQDQ"
     gene            complement(1631..6112)
                     /locus_tag="PKK03_07700"
     CDS             complement(1631..6112)
                     /locus_tag="PKK03_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84324.1"
                     /translation="MRKIIITILVAAAFAPALSAQIQQQFSGDTARFTTELSAFMGTL
                     VSKPQKAELDLFNKLYDSTVFSRPVKDRIINVASQLRGRRLSQVPGFIYFVRTLTDFV
                     ETEQKQDEVDVWLIGLSEMAFDPRFSNSSVEKFIEVTGLLLADNTIFSAGSVRWKATH
                     GKVEFARDTVLKIDIEAVTLTAYLGRDSTQILNFTGTYYPDIFRLHCQEGTVTWEKAG
                     YDPSRVFARISDFDIDVTKSEFTCDSALLTHPTYFQVPVPGKLTDRAVNIISPDKATM
                     PRFETYETRFFIDEIYRGVDYEGGLTLEGAIVRGTGSNWFPASVKLYRDDTLAIKITS
                     RNFILSQNSISSYESSPTLYLGTDSIYHSNLGFSYNTETREVSMSRTTSPLSRGPYFD
                     SYHDMDLYFEYLLWDMDQPTIIMSRTRGASIGSAKFESVTFYNEANFFRLMRLDDVHP
                     LYRVRDYAKQYGFDVFPVDGFAKSIRMPIEQATSLCIELANNGFLFYDRNFNEVAVKP
                     KVDDYIAAFAKKKDYDAITIFSETGDSEENAILNLKDYTIDVRGVTGVSLSDTQNVAI
                     RPYGGRLVIGRNRSISFDGIVHAGLFKIYGKDFSFNYDTFNIRLEKIDSIKIAVETGE
                     RDPYGRPVIRQIENMIELGSANLRIDDPKNKSGLRSMKQYPIINSTTDSYIFYDEITD
                     LDGLYPKEKFYFRLDPFTYTNIDHYTNEEMALAGEFVGGGIIEPMRQTLTVQPDTSLG
                     FTMTIPPEGIPIFDGRGRLYNHLSMSNSGLIGSGRVEHMTATAVADTFRFFPDYMLAR
                     AKTFDMKPDTEGRFPVLKAQDVDIQWLTDKDEWYAVNSEGKEFSIFGNGTSMAGTLIL
                     RPDKMKGQGEVNTADARITSETFSFGPMSFEADTSDYYLRALKGNGYGFVASDARTHI
                     DFTTRKASFRLNTDSSFVVLPGIEYISKMTGFEYDMEKKIMDMFQRGRESVPLMSPQE
                     LLKVPHGMEEKPTFLSTNNMKDTLKFQSGSASYFLQDEYLMVKDVNYIPVADALIQPG
                     DGVLYIERGARIRPTESAVVAVNNRHLLHSAKLNIESSANYSGSGKYDYISADGSSQV
                     IEFSDIRVDTMATKATGQIPANQNFTLSPAFTYSGDVRLRSDSEHLLFTGAAGIVTDC
                     GNISSKQVKFTAHIDPANVLIPISDKPRDLDDNLLFSGSFVTLDSAGVYGAFLSERHS
                     WSDAPLLNAEGFLFHDKGSDRYRIASMEKLADLRRHGNMVTFDRNMCLLLSEGKINFG
                     VDYGLLRLNSSGAVTHNTDSSRVELRSILGFSFYFNAEALKAMAADIRTNPTLRTVNL
                     SSEFNQKAMRDLLGEEAAKKLSEELQLFGVVRSLPKEYTSQLLLNDVTLQWNPYSMSF
                     ISKGKIGIGFIGDQPMNVYVDGWVELQRKRSGDLLDIYLKAGDNTWYWFSYFRGILMS
                     YSSNQAYNDILTNTKEKLRRDPNANSRIDFEYMPGVPDRLRSFLRRMESGGLGFEEY"
     gene            complement(6120..6878)
                     /gene="tpiA"
                     /locus_tag="PKK03_07705"
     CDS             complement(6120..6878)
                     /gene="tpiA"
                     /locus_tag="PKK03_07705"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNX84325.1"
                     /translation="MRKKIVAGNWKMNMNLNEGLELARGLNNHFISNPIGEKEVILCT
                     PFIHLAMTSNLLTAPGVTAGAQNCNDHKSGAYTGEISAAMVKSTGAGSVIIGHSERRT
                     YYGETDELLAVKTGEALRNGLRVIFCCGEVLAERQGNIHFEVVKRQLDKGLFVLSAED
                     FSKCIIAYEPVWAIGTGVTATPEQAQEMHCFIRQMVRDKYGKEAASDTTILYGGSCKA
                     SNAAELFANPDVDGGLIGGASLVKEEFCSIVNAL"
     gene            complement(6901..8136)
                     /locus_tag="PKK03_07710"
     CDS             complement(6901..8136)
                     /locus_tag="PKK03_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNX84326.1"
                     /translation="MKLLIQIARILTGLVFAFSGFVKGIDPMGTAFKLGDYFSAFRFG
                     FLDDFTLPLSLLLCVVEFVTGMMLLSGALLKPASWMAAIFMAIFTPLTLILAIWNPVS
                     DCGCFGDAIVLTNWQTFFKNVIIIIPVVFVFITRNDETGVMPVKAGLNFTLAFIFLFL
                     LFMRINLAYLPVIDFRPYSIGTNIAEGMAVPNDAVPDKYDIRFIYEKDGVQQEFTLEN
                     YPADDTLWKFIDQKSVLISKGYEPPIHGFTLMTEQGVDMTEQVTRQNGDVMLMVARRL
                     DESDRDGLMKGYSLGIELQKRGTDFHILTATSPAEAGPLVTGFSTLYADEITLKTIIR
                     SDPGFVLLHNGTVAAKWSYHNLPDPEKFMDGDLNALALRSRIVAENRLIAICAVMSVL
                     LAVALTLPFRMKKTIENNR"
     gene            complement(8133..8822)
                     /gene="tmk"
                     /locus_tag="PKK03_07715"
     CDS             complement(8133..8822)
                     /gene="tmk"
                     /locus_tag="PKK03_07715"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNX84327.1"
                     /translation="MKLIVIEGLDGAGKSTQISLLKEWFATRQMQCRYIHFPRTDAPY
                     FGELVARFLRGEFGDISSVDPYLVALLYAGDRKDAAAMIETWLEEKYFVVLDRYTYSN
                     IAYQCAKMNTEDDVRRLKEWILKLEFEHFGIPKPDINIFLDVPFRFTEEKLRNRRLGD
                     DRSYLLGSSDIHESSLEFQKKVRDIYISVAEDDQNLQVINCHDEDNRMPAPEEIFEMI
                     LDKLKERNILR"
     gene            complement(8841..9182)
                     /locus_tag="PKK03_07720"
     CDS             complement(8841..9182)
                     /locus_tag="PKK03_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS sensor protein"
                     /protein_id="HNX84328.1"
                     /translation="MKGIDWAEELGVAITVSDTDGKILYMNGKSASTFAKQGGRALVG
                     SGLDACHKAESWNKILQILDTGNANCYTIEKNGIKKMIYQAPWYSDGTVAGLVELSME
                     IPFNMDHFVRS"
     gene            9299..10153
                     /gene="folP"
                     /locus_tag="PKK03_07725"
     CDS             9299..10153
                     /gene="folP"
                     /locus_tag="PKK03_07725"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNX84329.1"
                     /translation="MNINVRGRLMDLSRPRVMGIINVTDNSFFQGSRYMQDDDIVRAA
                     AMMLEHGADILDIGGCSTRPGSEEVPEDVERERVCRAAGLILGKFPEAVISVDTYRSS
                     VAAAAVSDYGAAMINDISGGELDEGMFPLVARLNVPYILMHMQGTPRTMQVNPHYDDV
                     VADILAWFSRRMAPLRESGVRDIILDPGFGFGKKADHNFEMLRRFSEFRIAGMPLVAG
                     LSRKTMIWKTLDISPDLALNGTTALNMAALMKGASILRVHDVSEARQVVLLFEKMYPT
                     GIQFDHYS"
     gene            10180..10950
                     /gene="cdaA"
                     /locus_tag="PKK03_07730"
     CDS             10180..10950
                     /gene="cdaA"
                     /locus_tag="PKK03_07730"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNX84330.1"
                     /translation="MFQITVLDIIDILMVAIIFYQLYRLIRGTAAFSIFLGIFFVYLF
                     WLVVKALNMELISAIIGQVIGVGVIALIIVFQQEIRRFLLAIGNRYMSRNRFSFDRYF
                     PSGEVDQTTSQIVEEIVHAVESMAQSRTGALIAVGRTSLLDIYSDGGELLNALVTDEL
                     LKTIFYKGSPLHDGAVLIQNGKILAARCPLPSTDQTNLPARFGMRHRAGIGLTEQTDA
                     MVIVVSEERGRISVADAGKIQEDVTPNELRQVLLTIRI"
     gene            complement(11019..11438)
                     /locus_tag="PKK03_07735"
     CDS             complement(11019..11438)
                     /locus_tag="PKK03_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84331.1"
                     /translation="MSKTSLILVLLIMLLFSCEEGFLTDCDKCYTGPDFSVELNVIYR
                     NPDRVPVNPLLTIYEGNISDSIIISRIRIADPYSYMYQPAILYKDYSATLEFTYDGQK
                     YLTTASACPGVRYDETTCDEPCYFVYDNVLDLRLRYD"
     gene            complement(11431..12834)
                     /locus_tag="PKK03_07740"
     CDS             complement(11431..12834)
                     /locus_tag="PKK03_07740"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNX84332.1"
                     /translation="MGRIKEILEIYNARTLYVDTSEYVHGFEDADNINLQTAASMGAC
                     NEIIFLRARGADVNNTVGNIATPLHYAVNSGNWGAVEILLLLGANPDVKDIYGNTPLV
                     AAVRADQREIAEKLIRYGASIDETDRQLSSPLHHSVALRNFSMTDMLLYYDSPTELFD
                     IEGNTPLMTGVCFGYHDIADLLLQSGADPNAADRRGFTPLMAASQNGDTLMMSILVKA
                     GSNLYALNDDGVDALGCAVIGSQKDAADFLLSHGNRWNQTSGTRGDPVILANTSGESD
                     ILQMMNEHGLKAKREYTFDQLSFSAGGWITSHYQLAGGSISVSEPGLGAGITLGAAVN
                     PVNQRVLVGGDNSTIYQYNVKSNLIYAGLFREFPLNGKLNVLRVSVIPALNFGYMFHS
                     LYAGTEEKPDDKFCIIPEADIRFGWHNLSLNPGLAFMKSPFRKVGPLWFTLKVAYDLK
                     RPQDKIKGKRIRIYDYE"
     gene            13096..14391
                     /locus_tag="PKK03_07745"
     CDS             13096..14391
                     /locus_tag="PKK03_07745"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNX84333.1"
                     /translation="MRLLIIVFLVLFMVVPKVGTEQVAMIDGHYVPVNLRLDNSLSEN
                     DMFVNAEVGVEWFMKNWDIKGASVAVAKEGKLLYARGFGYASLDDSIPVEPYHRFRIA
                     SVSKLVTAVAIMQLQEEGRLSVNEKVFGPGAILDDTIFDHPRDKRAFDITVGHLLAHE
                     GGWSQRYGDQMFMPEVISRTLGVPMPVDLKTIIKFALTKNLHFTPGAGQSYSNLGYAI
                     LGLVIEEASGMDYESYCRKKVLEPLGIYDMALGKNLPENALPLEVSYYDVDNAPLRPS
                     IYGTGEMVRASRGGNDIETLGAAGAWVATAPDLMRLLLAVDGFEFPKDILSPESIEFM
                     TDAENGYAPVGWRATLSNGVWWRTGSFSGTSAMMKRMPDGTAWVVLMNSSGWNGPELT
                     GDIDQMMAKFLQRVSQWPDTDLFSYYTPVPLKFARVSEP"
     gene            14410..15132
                     /locus_tag="PKK03_07750"
     CDS             14410..15132
                     /locus_tag="PKK03_07750"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNX84334.1"
                     /translation="MCFTVNVNIVKEELEARYGSDLIDHDKYRPSYYYHAYSLPSMPV
                     VCSDRDAGIRLFRWGLIPSWVKDEGEANEIMLKTFNARSETISSKPAFRDSFASRRCL
                     VPVCGFFEWQHHGGRKIPWYITLRGENIFSLAGIWDSWTVEGGMTLNTFSVVTTRANE
                     LMEEIHNSKKRMPVILPASAERMWLRDGADESALAALMEPVAPDMLSAHTISPLITNA
                     RADRNRPELIMPFSYPVQGTLF"
     gene            complement(15138..>15635)
                     /locus_tag="PKK03_07755"
     CDS             complement(15138..>15635)
                     /locus_tag="PKK03_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84335.1"
                     /translation="ADKPLDIDPKEYEDMVKEEMIKSGLLNENNFIDQQKMAEEAGME
                     EIAATPEEVKPQVTEKKEKPQEQGTFRGPTRIFYDLAGRHHVYLPIPIYKCEGAGQIT
                     LAIVVDRGGNVLKAEPASKLSTTRDQCLTETAVEHALKTRFNADQSAPEKQGGFLTFV
                     FVSQN"
BASE COUNT         3705 a         3874 c         3930 g         4126 t
ORIGIN      
        1 ttgaatggat ccttgaccag gcactgcgca agtccggcag gcttcgtgac aacagggaaa
       61 gatcaaatca gaaaaaaaat gaaaagtgaa atgcccatga taaagtatac ctgcccgaaa
      121 tgtggtggca ggaagtacag gctcagcgag ataagggttg cacacagcct ttttacacag
      181 ctttttgaca ttcaggcgag gaaatattca gccatgatct gtgaaaaatg cagttataca
      241 gagttctata acgtaccggt aaagaagata acaagcgtct ttgatttctt taccagcacc
      301 tgatctcaga tgtttgccag gtaaacggag cagcaggcag cgatgcctgc tgtttcagtt
      361 ctgagacggc ctgtgccaat atgcactggt acataaccgg cgctgatggc agctttaacc
      421 tcttcggggg tgaaatcgcc ttcaggaccg atgagaatcg ttacattatc accccggtta
      481 aaagcttccg ttatgcctct cctgtcaaat gaagagtcgc atgtggcaat aatccttttc
      541 cctttcgccg gtctgcgcgt aaattcctct gctgtttccg gttcctcaag tcttgggagg
      601 atgcaccggc ccgattgctt cattgccgat atgattattc cttccagtct ttcccttctt
      661 attcttttct tttcggaacg gctgcagata acaggtgtta tgacgtcaac cccgatctcc
      721 accgctttct cgacaaacca ttcgagcctg tcttcattct tcaggggtga tattgccaca
      781 tgaagcctgt attttccggg taaggggtca ctttttgctg atgtgatggt gactgacatc
      841 ccttttgggt catctgtggc gatcactccg tcgtacaggt ttcccctgcc gtcggtaaag
      901 gtcagggtgt ctccgcggcg cattctcagc accctgaggc agtggcctga ttcttccctg
      961 ctgaagacag ctttgtcacc ggagatatct tcacaataaa aaatctgcat cctgtcgatt
     1021 gtttgcgcaa atttacggat tttagcgttg tcataatttt caaatccgga caatgaggat
     1081 aggagtgata tcagacacgc acggctggat tgatcctgct gtttacgagc acttttccgg
     1141 tgtcgatgag atctggcatg caggtgacgc cggtaatgcc ggtgttatcg ccgaacttgt
     1201 ggcgttcagg ccgttgcgtg ccgtctgggg caacattgac gggtttgacg tcaggaggga
     1261 aacgaaggaa tatctcttct tcggtgcagg ctcgaagaat gtgcttatga ttcacatcgg
     1321 aggctatccc ggcaggtatg caccccgggc gctggagctg atcagggagc ttagaccgga
     1381 tatctttgtc tgcgggcact ctcacatcgc aaaggtgatc tatgacaaga ccttcgggat
     1441 gctctgcatt aatcccggtg cggcaggccg aaccgggata cataaggtaa tgaccatgct
     1501 ccggttcact atagatggcg caaatgtcag tgatatggag gtcattgaat tcggcagcag
     1561 aggacgcagc caggatcagt gagaccgccg gatccttcat gaaacaggcc ctgccgttgt
     1621 tgcaggcggg ctaatactcc tcgaatccca gtcctccgct ctccattctt cgcaggaaac
     1681 tcctgagcct gtcaggcacc cccggcatat attcaaagtc tatcctggaa ttggcattcg
     1741 gatctcgcct cagcttctcc ttcgtgtttg tgagtatgtc attatatgcc tggtttgagg
     1801 aatagctcat cagtattccc ctgaagtatg agaaccagta ccaggtgttg tcacctgcct
     1861 taaggtagat atccagcagg tcgccagacc ttttcctctg aagctccacc catccgtcaa
     1921 catagacatt catgggctgg tcaccgataa atcctattcc gatttttcct tttgatatga
     1981 aagacatcga atagggattc cactgaagcg ttacgtcgtt aaggagcaat tgcgaggtgt
     2041 attccttcgg cagtgagcgt accacaccga agagctgaag ctcctcgctg agttttttag
     2101 cagcctcctc gcccagaaga tcacgcatgg ccttctggtt gaactcggag gagagattga
     2161 ctgtcctgag tgtcggattg gtccgtatat cggcagccat ggctttaagc gcttcggcgt
     2221 tgaaatagaa tgagaatccc agtatggaac gcaattccac tcttgatgag tcggtattgt
     2281 gtgtgacggc cccggaactg ttaagcctga gcagtccgta gtcaactccg aagttgatct
     2341 tcccttcact cagaagcagg cacatgttcc tgtcaaatgt caccatgttc ccgtgcctgc
     2401 ggagatcagc aagcttctcc attgacgcga tccggtacct gtcactgccc ttgtcatgga
     2461 acaggaagcc ttcggcattc agcagcggag catcgctcca cgaatgcctt tcagacagga
     2521 aggcgccata gacaccagct gaatcaagtg tcacaaaact tccggaaaag agaagattgt
     2581 catcaaggtc acgtggcttg tcactgatag gaataagcac atttgccgga tcaatgtgag
     2641 cagtgaattt aacctgtttg ctgctgatgt tcccgcagtc ggtcacaatt ccggcagcac
     2701 cggtgaaaag caaatgttcg ctgtcagatc tcagtctcac gtcaccggaa taggtgaaag
     2761 caggactgag ggtaaagttc tggtttgccg gtatctggcc ggtggctttg gtggccattg
     2821 tatcaaccct gatatcgctg aattcaatta cctggctgct gccgtcagcg cttatatagt
     2881 catatttgcc cgacccgctg tagtttgcac tgctttctat gttcaatttt gccgagtgca
     2941 gcaggtgtct gttgttgacg gcgaccaccg cactctcagt aggcctgata cgtgcccccc
     3001 gttctatgta aagcactcca tcgcccggtt gaatgagagc atcggcaacg gggatataat
     3061 tgacatcctt caccatcagg tattcatcct gcaggaaata tgacgctgag cctgactgga
     3121 atttcagggt atccttcatg ttgtttgtgg agaggaatgt gggcttctcc tccatgccat
     3181 gaggcacctt cagcaattcc tgcggtgaca tgaggggaac agattccctg cctctctgaa
     3241 acatatccat gatctttttc tccatgtcat actcaaagcc ggtcatcttt gatatgtact
     3301 ctatccccgg cagcaccaca aatgaagaat cagtattaag ccggaatgat gctttccggg
     3361 tggtgaaatc gatatgagtc ctggcatcgc tggccacgaa accatatccg ttgcctttaa
     3421 gtgcccggag gtaataatcg gaagtgtcag cctcgaatga catcggcccg aaggagaatg
     3481 tttcagaggt gatccgggca tcagcagtgt tcacctcacc ctggcctttc atcttatcag
     3541 gccggaggat gagggtaccg gccatggagg ttccgtttcc gaatatgctg aattcctttc
     3601 cctcactgtt caccgcatac cattcgtctt tgtcagtgag ccactgtatg tccacatcct
     3661 gtgctttgag gaccgggaac ctgccttcag tatccggctt catatcaaag gttttcgccc
     3721 tggccagcat gtaatcaggg aaaaacctga atgtgtcggc cacggctgta gccgtcatat
     3781 gttcaaccct gccgcttccg ataaggcccg agttgctcat gctgaggtgg ttgtacagcc
     3841 ttccccttcc gtcaaatata ggtatcccct ccgggggaat ggtcatggtg aatcccagtg
     3901 acgtgtcagg ctggactgtc agggtttgcc gcatgggctc aataattccg ccgccgacaa
     3961 actccccggc aagagccatc tcctcattgg tgtaatggtc gatattggta tacgtaaacg
     4021 gatcgagcct gaagtagaac ttttccttcg ggtacaaccc gtcaagatct gttatttcat
     4081 catagaagat ataggaatca gtggttgaat tgatgatcgg atactgcttc atgcttctga
     4141 gccccgattt gttctttggg tcatcaatac gcaggttggc tgaaccgagc tcgatcatat
     4201 tttcgatctg cctgatcaca ggcctgccat aagggtctct ctccccggtt tccactgcta
     4261 tcttgatcga atcaatcttc tcaagcctta tattgaaggt atcataattg aatgagaagt
     4321 cctttccgta gattttaaaa agtccggcat gcacaattcc gtcaaagctg atcgatctgt
     4381 tcctgccaat gaccaggcgg ccgccgtagg gccggatggc cacgttctgc gtatcactga
     4441 gcgaaactcc tgttactccc ctgacatcaa tggtgtaatc tttaaggttc agtatggcat
     4501 tttcctcact gtctccagtc tcgctgaaga tggttatcgc gtcatagtcc ttctttttgg
     4561 cgaaggctgc aatatagtca tcaaccttag gctttaccgc cacctcgttg aagttgcggt
     4621 catagaacag gaagccgttg tttgccagtt caatgcataa agacgtagcc tgttcaatgg
     4681 gcatacgtat ggattttgca aaaccatcaa ccgggaagac atcaaaacca tactgtttgg
     4741 cataatccct taccctgtac aggggatgaa cgtcatcaag cctcatcagc ctgaaaaagt
     4801 ttgcctcatt gtaaaaggtg actgattcga actttgctga ccctattgaa gcgcccctgg
     4861 tccttgacat gattatggta ggctggtcca tatcccacaa cagatactcg aaataaagat
     4921 ccatgtcatg gtatgagtca aagtacggac cccttgacag ggggcttgtg gtgcgggaca
     4981 tgctcacctc cctcgtctcg gtattatatg agaacccgag gtttgagtga taaatggagt
     5041 cagtccccag gtacagggtg ggagacgact cgtaactgct gattgagttt tgtgacagga
     5101 tgaagttcct ggaggtgatt tttatggcga gggtatcgtc acggtaaagt tttacgcttg
     5161 ccgggaacca gtttgagccg gtgcccctga caatggcgcc ctccagggtc agccctcctt
     5221 cgtagtcgac acccctgtag atctcatcga tgaagaacct tgtctcatag gtctcgaagc
     5281 gaggcattgt ggccttgtca ggagaaatga tattgacagc cctgtcagtc agtttacccg
     5341 gcaccggcac ctggaagtat gtcgggtggg tgagaagtgc cgagtcgcag gtaaattcac
     5401 ttttggtaac atcaatatca aaatccgata tcctggcaaa gacccttgac ggatcataac
     5461 cggctttctc ccaggtgact gttccttcct ggcaatggag ccggaagatg tcagggtaat
     5521 atgtcccggt gaaattcagt atctgtgtgc tgtcacgtcc aaggtatgcg gtcagcgtaa
     5581 ctgcttcgat atcaatttta agtacagtat cacgcgcaaa ctcaaccttt ccgtgggtgg
     5641 ctttccacct gacagatcct gcagaaaaga tggtattgtc cgccagcagg agaccggtca
     5701 cctctatgaa tttttccacg gaggaatttg agaaacgggg atcaaaggcc atttcactca
     5761 gtccgatcag ccagacatcc acctcatcct gcttctgttc agtctccacg aagtcagtca
     5821 gggttctgac gaagtatatg aagcctggta cctggctcag cctgcgcccg cgcagctggc
     5881 ttgcaacgtt aatgatccgg tcctttacgg gccttgaaaa taccgtactg tcgtacagtt
     5941 tgttaaagag atcaagttca gccttttgcg gtttgcttac cagggtgccc ataaatgcag
     6001 acagttcggt ggtgaacctg gcagtgtcac cggagaactg ctgctgaatc tgtgctgaaa
     6061 gcgcgggagc gaaagcagca gcaaccagga tggtgattat gatctttctc atggactttt
     6121 cagagggcgt tcacgatgct gcagaactcc tcctttacca gtgaagcccc gcctatcagg
     6181 cctccgtcaa cgtcaggatt ggcgaaaagc tctgcagcat tggatgcctt gcagctaccg
     6241 ccataaagta tggtggtatc actggctgct tcctttccgt atttgtccct gaccatctgg
     6301 cggataaagc agtgcatttc ctgggcctgt tcaggggtgg ctgttactcc tgttccgata
     6361 gcccatacag gttcgtaggc gatgatgcat ttgctgaagt cttctgctga caggacaaac
     6421 aggcccttat ccagctgtcg tttcacaact tcgaagtgga tattgccctg cctttctgca
     6481 aggacctcgc cgcagcagaa aataaccctg agaccgtttc gaagggcctc gccggtcttg
     6541 acagccagca gctcgtctgt ttctccgtaa taggttcgtc tttcagagtg tccgataatg
     6601 acactgcctg ccccggtgct cttaaccatt gcagcagaga tttctccggt ataagcgcca
     6661 gacttgtggt cattgcaatt ctgggctccg gcagtcacac ccggtgctgt cagcagattg
     6721 ctggtcattg ccaggtgaat gaacggagtg catagtatta cttccttttc gccgatggga
     6781 ttggagataa aatgattgtt cagccccctc gctagctcca gcccctcgtt caggttcata
     6841 ttcatcttcc agtttcctgc aactattttc tttctcattg tcgttttatt gttagagtta
     6901 ttatctgttg ttttcaattg tttttttcat cctgaaggga agggtaagtg ctacggcaag
     6961 gaggacagac atgacagcgc atatggcaat aagcctgttt tctgcaacta tgcgtgatct
     7021 gagagccagc gcgttgaggt caccatccat gaatttctcc ggatcgggaa ggttgtggta
     7081 tgaccacttg gctgccactg tgccgttgtg caggagcacg aagcccgggt cagatcttat
     7141 gatagttttc agggttatct catcagcata cagtgtgctg aatcctgtaa ccagcggacc
     7201 cgcttctgca ggggaggtag ctgtcaggat atggaagtca gtgcctctct tctgcagctc
     7261 aatacccagg ctgtaacctt tcatcagccc gtccctgtca gactcatcaa gccggcgtgc
     7321 aaccatcagc atcacgtcgc cgttctgccg ggtaacctgc tcagtcatgt caacaccctg
     7381 ttccgtcatg agagtgaatc cgtgaatggg aggctcgtat ccctttgaga tgagaactga
     7441 tttctgatcg atgaatttcc acagtgtgtc atctgctggg tagttttcaa gggtaaactc
     7501 ctgttgcact ccatcctttt cataaatgaa ccggatatca tatttgtcag gtacggcatc
     7561 gtttggaaca gccatgcctt cagcaatatt tgtcccgatg ctgtaaggcc tgaaatcgat
     7621 caccggaagg tacgcgaggt ttatcctcat gaaaaggaga aacaggaaaa taaatgccag
     7681 cgtgaagttg aggccggctt ttaccggcat gactccggtc tcatcgttcc tcgtgatgaa
     7741 cacgaacaca accggaataa taatgatcac attcttgaag aatgtctgcc agttggtgag
     7801 cacaattgca tcaccgaagc atccgcagtc agagaccgga ttccatatgg caagaataag
     7861 tgtcaggggg gtgaatatgg ccatgaaaat ggctgccatc catgatgccg gctttagcaa
     7921 cgctccggac aggagcatca tacccgtaac aaattcaacg acgcagagca gcagtgagag
     7981 gggaagggta aagtcatcga ggaaaccaaa tctgaatgcc gaaaagtaat cgcctaactt
     8041 gaatgccgtg cccatcgggt caattccttt aacaaagccg gagaaagcaa aaaccagccc
     8101 tgtgagtatc ctggcaatct gtatcagcaa cttcatctca gaatattcct ttcttttagt
     8161 ttgtcaagaa tcatttcgaa aatctcttcc ggtgcaggca tgcggttatc ctcatcatgg
     8221 cagtttatga cctgcaggtt ttgatcatcc tcagctacac taatgtagat atcgcgtacc
     8281 ttcttctgga actcgaggct gctctcatgt atatcactgc ttccaaggag ataactcctg
     8341 tcatcaccga gcctcctgtt cctgagcttc tcctcagtga acctgaaggg aacatcaagg
     8401 aaaatattga tatccggttt cgggattcca aaatgctcaa attccagttt cagtatccac
     8461 tctttcagtc ttctcacatc atcctcagtg ttcattttgg cgcactgata ggcaatgttg
     8521 gaataagtat aacgatcaag aacgacgaag tatttctctt cgagccatgt ttctatcatt
     8581 gcagctgcat ctttcctgtc gcctgcatag agcagggcaa caaggtaagg atcgacgctg
     8641 ctgatgtctc cgaactcgcc tctcaggaac cgggcaacca gttcgccaaa gtagggagcg
     8701 tctgtgcgag gaaagtgaat atatctgcac tgcatctgcc tggttgcaaa ccactccttc
     8761 agcaatgaga tctgggtaga tttgccggcg ccgtcaagac cttctattac aattagtttc
     8821 atggcctggg ggatttgttt tcagctgcgg acaaagtgat ccatattaaa cggaatctcc
     8881 atcgaaagtt caaccagccc cgcaacggtt ccgtcgctgt accacggagc ctggtaaatc
     8941 atctttttta tgccgttttt ttcaatggtg tagcagtttg cattcccggt gtcaagtatc
     9001 tgcaggattt tgttccatga ttcagctttg tggcacgcat ccaggccgga acccaccagt
     9061 gcccgtcctc cctgctttgc gaatgtggag gctgatttcc cgttcatgta aagtatcttt
     9121 ccatccgtgt cagaaactgt tattgccaca cccagttcct cagcccagtc tatgcctttc
     9181 atatgtattg tctgaattta cagaaagcaa atatacttaa tttgacggca acggtaagaa
     9241 ctctgcgcta tcagtacggg gccaatgact atctttaccc tggaataaaa caacaggcat
     9301 gaatatcaat gtaaggggga ggctgatgga tctttccagg cctcgtgtga tgggcatcat
     9361 aaacgtcact gacaactcgt tctttcaggg cagccgctat atgcaggatg atgatattgt
     9421 cagggcagct gccatgatgc tcgagcatgg cgctgatatt cttgatatcg gcggatgctc
     9481 cacccgtcct ggttctgagg aggtgcctga agatgttgaa agggaacggg tatgcagggc
     9541 tgcaggattg attctcggga aatttcctga ggcagtaata tcagttgaca cctacaggtc
     9601 ttcggttgct gctgctgccg tttccgatta cggcgctgca atgataaacg acatttcggg
     9661 cggagagctt gacgagggga tgtttcccct tgttgcccgg ctgaatgtgc catatatact
     9721 tatgcatatg cagggcacgc cccggacgat gcaggtgaat cctcattatg atgatgtcgt
     9781 cgcagacatt cttgcctggt tttcgcgacg aatggcgccg ttgagggagt ctggcgtcag
     9841 ggacatcata cttgatccgg gcttcgggtt tggcaagaaa gcggatcata attttgagat
     9901 gctgcgcagg ttctccgagt tccggattgc gggcatgccc cttgttgccg gcctttcaag
     9961 gaagaccatg atctggaaga cccttgacat ctctcccgat ctcgcgctca acggcacaac
    10021 agccctcaat atggctgcac tcatgaaagg agcttccata ctgagagtcc atgatgtcag
    10081 tgaggcacgg caggttgtgt tactttttga aaagatgtac ccaacgggca tccaatttga
    10141 tcattattct taaatttgtc ggtcactgca ctctgacata tgttccagat cactgtcctc
    10201 gatatcatcg atatattaat ggtagcaatc attttctacc agttgtaccg tcttatcagg
    10261 ggcactgctg cattcagcat ttttctcggg attttcttcg tctatctgtt ctggctcgtg
    10321 gtgaaggctc tcaatatgga gcttataagc gccataatag gacaggtgat cggggtaggg
    10381 gtcattgcgc ttatcattgt gttccagcag gagatcagaa gatttctttt ggctatcggg
    10441 aacaggtata tgagccggaa caggttctca tttgaccggt atttcccgtc aggagaggtg
    10501 gaccagacca ccagccagat tgttgaggag attgttcatg cagttgaatc aatggctcag
    10561 tcgcgcacgg gtgcacttat tgccgtgggt cgcaccagtc tccttgacat ttactctgac
    10621 gggggtgagt tgctcaacgc tctcgtaact gacgagctcc tgaagacaat tttttacaaa
    10681 gggtcaccgc tgcacgacgg ggcagtgctt atacagaacg ggaagatact tgccgcgcgc
    10741 tgcccgctgc catccaccga ccagacaaac cttccggcac ggtttggcat gcggcaccgc
    10801 gccggaatcg gccttacgga gcagactgat gccatggtca tcgtcgtatc cgaagagaga
    10861 ggaaggatat ccgtagccga tgccgggaag atccaggaag atgtgacacc caatgagctc
    10921 aggcaggtac ttctgactat cagaatttga tgcatttgac tttaaagcct gtgtccagtc
    10981 agcacagatg cgcggggaac agccttccgc gggcactgtc agtcatacct tagcctcagg
    11041 tcaagcacgt tgtcataaac aaagtaacag ggttcatcac aggtcgtctc gtcataccgt
    11101 accccggggc aggctgaggc agtggtgagg tatttctgac cgtcatacgt gaactcaagg
    11161 gtggctgagt aatctttgta aagtatagcc ggctgataca tataggagta cggatccgcg
    11221 atacggatcc ttgatatgat gatactgtcg ctgatgttcc cttcataaat tgtgaggagc
    11281 ggatttaccg gaaccctgtc aggatttctg taaattacat tgagttcaac actgaagtca
    11341 gggccagtgt aacacttgtc acaatcagtc aggaaacctt cttcgcatga aaagaggagc
    11401 attatcagga gtaccagaat gaggctggtt ttactcatag tcgtagattc tgattcgttt
    11461 gcccttgatt ttgtcctgag ggcgtttgag gtcataggcc accttaaggg tgaaccataa
    11521 tggtccaacc ttgcgaaacg gtgatttcat gaaagcaagg ccgggattca ggctcaggtt
    11581 gtgccagccg aaccggatgt ctgcctcagg aataatgcaa aatttgtcat caggcttttc
    11641 ttcagttccg gcgtaaagtg aatgaaacat gtatccgaaa ttcagggccg gaataacgct
    11701 gacccgcagc acgttcaact taccgtttag aggaaattcc ctgaacaggc ctgcgtagat
    11761 gaggtttgat tttacattat actggtaaat tgtgctgtta tccccgccga ccagaacacg
    11821 ctgatttacg gggttaacgg cagcgccaag agttattccg gctccaaggc cgggctcaga
    11881 aacggaaatt gaccctcctg caagctgata atgtgaagta atccatcctc ctgctgaaaa
    11941 tgagagctgg tcaaaggtat actcccgctt tgccttcagc ccgtgttcat tcatcatctg
    12001 aagaatatct gactcacctg aagtgtttgc cagtatcacc gggtcgccac gcgtaccact
    12061 ggtttggttc catcggtttc catgtgagag cagaaaatct gcagcatctt tctgtgatcc
    12121 tatcactgcg cagccgaggg cgtcaacccc gtcgtcattc agcgcataaa ggtttgaccc
    12181 tgcctttacc agtatgctca tcataagcgt gtctccgttc tggcttgctg ccatcagagg
    12241 cgtaaaaccc cgcctgtcag cagcgttggg atctgcgcct gattgcagca ggaggtccgc
    12301 aatgtcatgg taaccaaagc atactccggt catcagggga gtgttccctt caatgtcaaa
    12361 cagctccgtg ggagagtcgt agtaaagaag catgtctgtc atggaaaagt ttcgcagcgc
    12421 cacggaatgg tgcaatggcg aagagagttg cctgtcagtt tcgtcgatcg atgcaccgta
    12481 cctgatgagt ttttcggcaa tttcccgttg atcagcccgg acagctgcaa caagcggcgt
    12541 gttcccgtag atatctttca catcgggatt ggctccaaga agcagcagta tctccacggc
    12601 accccaattt cccgagttga ccgcatagtg gagaggagtc gcaatattcc ctacggtatt
    12661 atttacatct gcccctctgg ccctcaggaa tatgatctca ttgcatgcac ccattgatgc
    12721 agcagtctgc aggtttatat tgtctgcatc ttcaaatccg tgaacatact cagaggtgtc
    12781 aacatatagc gtgcgtgcat tgtaaatctc caggatttcc ttgattctcc ccattattga
    12841 atcaataacc aggctgtcgg tctggccact caggtctaaa cagcagatca gcagaggaac
    12901 caggaataat gactttctta aggaatgcct catgcataca aatttactct cagattacga
    12961 cccttccaat tttgttgata ttttttttct ccggttaaga aaaatgttgc cggaaggggt
    13021 tgcgagcagg ataatattct aattttaccg ccgttttctg agaagtacac tcttatgatt
    13081 ctgcctgtaa aaccaatgcg actgctgata atagtcttcc tggtgctatt catggttgtg
    13141 cccaaggttg gcacggaaca ggtggcaatg attgacggcc actatgtccc tgttaacctg
    13201 aggctagata actccctgtc tgagaatgat atgtttgtaa atgcggaagt gggggtggaa
    13261 tggtttatga agaactggga cataaagggt gcatcggttg ctgtggcaaa ggaagggaag
    13321 ctgctgtatg cacgaggttt tggctatgct tcacttgatg attcaattcc tgttgagcca
    13381 tatcaccgtt ttcgtattgc aagcgtctcc aagctggtaa ctgcggtggc aatcatgcaa
    13441 ctgcaggagg agggcaggct ttcagtgaac gaaaaggtgt tcggccccgg cgcaatactt
    13501 gatgacacta tttttgacca tcccagggac aagcgtgcat ttgacatcac ggtcgggcat
    13561 ctcctggctc atgagggtgg ctggtcgcaa aggtatggtg atcagatgtt tatgcctgaa
    13621 gtaatttccc gtacccttgg ggttccgatg cctgtggatc ttaagaccat aataaagttc
    13681 gcactgacaa aaaacctgca tttcaccccc ggagcagggc agtcatacag caatctcgga
    13741 tatgccatcc tgggcctcgt catcgaagag gcttcgggga tggattatga atcctattgc
    13801 aggaagaagg tgcttgaacc gctgggaatc tatgacatgg cactcgggaa aaacctgcct
    13861 gagaacgccc tgcctcttga ggtctcctac tatgatgtcg acaatgcacc tctcaggcct
    13921 tccatctacg ggaccggcga gatggttcgg gccagcaggg gaggaaatga catagagacc
    13981 ctcggagcag ccggagcatg ggtggccact gctcctgacc tgatgagact gctccttgct
    14041 gtggatggct ttgaattccc gaaggatatc ctctcacctg aaagcataga gttcatgacg
    14101 gatgcggaga acggatacgc ccctgtaggc tggagggcca cactctccaa cggcgtgtgg
    14161 tggcgtaccg gttcattctc gggcacttca gcaatgatga agaggatgcc tgacggtacc
    14221 gcatgggtgg tacttatgaa cagcagcgga tggaacggtc ccgaactgac aggcgacatt
    14281 gaccagatga tggctaaatt cctgcaacgg gtcagccagt ggcctgacac tgacctgttc
    14341 agctattaca cgccggtacc gctgaaattt gccagggttt ctgaaccctg atcaagaatc
    14401 aactccacga tgtgcttcac tgttaatgta aatatcgtca aggaagagct cgaagcacgt
    14461 tacggcagtg acctcattga tcacgacaag tacagaccga gttactatta ccatgcctac
    14521 agcctccctt caatgcccgt ggtttgttct gaccgggatg cagggatcag gctattcaga
    14581 tggggactta taccatcatg ggttaaagat gagggtgaag ccaatgagat tatgctcaag
    14641 accttcaacg cccgttccga gaccatctct tccaagcctg ctttcaggga ttcatttgca
    14701 tcgcgccgtt gtctcgttcc ggtatgcgga ttcttcgaat ggcagcatca cggcggtaga
    14761 aagataccgt ggtatataac cctcagggga gagaatattt tctcactggc aggaatctgg
    14821 gactcatgga ccgtggaggg cgggatgacc ctgaatacat tttccgttgt caccacccgt
    14881 gccaatgagc tcatggaaga gatccacaat tcaaaaaaga ggatgccggt tatccttccc
    14941 gcttcagcgg aacggatgtg gctcagggat ggagcagatg aaagtgcact ggctgcgctc
    15001 atggagcccg tggccccgga catgctctca gcccatacga tcagccccct gataaccaat
    15061 gcaagggctg acagaaacag gcctgaactt atcatgcctt tcagttaccc tgtacaggga
    15121 acacttttct gatcagatta attctgcgat acaaacacga aggtgaggaa gcctccctgc
    15181 ttttcgggag cgctctggtc agcattgaac cttgttttaa gtgcatgttc cactgctgtc
    15241 tcggtgaggc actgatccct ggtggtggaa agttttgagg ccggctcggc ctttagcaca
    15301 tttccgccgc ggtcaaccac aatggcgagc gttatctgac ctgcaccctc acacttgtaa
    15361 ataggtatag ggaggtagac atggtggcgg ccggcaagat cataaaaaat ccttgtggga
    15421 ccccggaaag tcccctgttc ctggggtttc tccttcttct cggtgacctg gggctttacc
    15481 tcctcaggtg tggcagcaat ctcttccatc cctgcctcct ccgccatctt ttgctggtca
    15541 atgaagttat tttcattaag aagaccgctc ttgatcatct cctccttgac catgtcctca
    15601 tactccttgg gatcaatgtc cagaggcttg tcagc
//
LOCUS       DAOPXU010000064        15374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_4142_length_15374_cov_14.594686, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000064 DAOPXU010000000
VERSION     DAOPXU010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15374
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4142_length_15374_cov_14.594686"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1896)
                     /locus_tag="PKK03_07760"
     CDS             complement(76..1896)
                     /locus_tag="PKK03_07760"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNX84336.1"
                     /translation="MSYAQSFNTFPVTTQKPPLHGRHWMAITGKPLAATAGAMIFDRG
                     GNAVDAACAMLAASCTMWDVLSWGGETQALIYNPKTGKVLAINALGVAPTGATAEFYK
                     SKGYKYPPEYGPLAAVTPGTPGGLMVMLAEYGTMSLREVLAPAMEMAAGYPMEAQTAN
                     SIERAKNRIKDWPYSARVFLPHGGEEREAPSAGEVFVQKDLLATLTKLVEAEQQALKK
                     KKSRRDAIYAAYDRFYNGDIAAEFARGCQEQGGLITKEDLAGWQVKIEEPLMTEYKGI
                     EVYKLQQWTQGPVMLQTLNILENFNLKELGYNSTRYIHTLYQAMNLAFADRDFYYGDP
                     SFPPEEPMQGLLSKDYARERSKLINPDRNDPAVRPGDPYLFEGKVNPFSTVLDSWQAD
                     ARNIYPSYDPEQYSSFMSKFQSGTTSIEAADEEGWVVSVTPSGGWVPACIAGNTGIGM
                     SQRMQSFVLDAKENPFNVVEPGKRPRVTLTPTLAMKDGKPLLSFAKQGGDEQDQLLLQ
                     FFLNVVEFGMTVQEACEAPSFKTLQMYSSFGEHEKVPGGLVLNSAMPSWVRKELAAMG
                     YRLSFQERTSGPVNAIGFDRAHGTFWGGSSNHGEDYGIGW"
     gene            complement(2050..2727)
                     /locus_tag="PKK03_07765"
     CDS             complement(2050..2727)
                     /locus_tag="PKK03_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84337.1"
                     /translation="MEREKPTGLIEIGLAAALLFSVLYGIGVRYLGGPDAFRYYVRED
                     GPAEYATAFFLFAGSLVAIFRAFTCCRGKRHLPVLTWSVLSFLFFFAAGDEISWGQRI
                     FNIESSEFFLKYNKQAETNIHNLVVAGKSINMIIFSRMVLIALLFYFIILPVLAKKPG
                     LIRRLVEKFGLPLPSLRHIIIMGVSTALIAVIRMAKGSELNELAFSVIFFLVFLNPRT
                     VGREQLS"
     gene            2913..3998
                     /locus_tag="PKK03_07770"
     CDS             2913..3998
                     /locus_tag="PKK03_07770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HNX84338.1"
                     /translation="MNPGLYFWFAFILIFIVGFWVDMRAAKNRKSEESVATALRWTGF
                     WITVAFLFGLAIFLFYPQNEGSAISTRNIMGMKFYSAYLTEYMLSIDNLFVFIMIFTM
                     MKVPGDIQPILLKYGILLSIFFRTLFILVGMGLINRFEWLIYLFGAFLIYMTFKMMKS
                     SDEEDTIDPEKNTLIRAAKKVFQVDVNNKNPRFHSRLNGKFAITPFALTFLLIGSTDV
                     MFAVDSVPAVIGVIREGNQALTLYESNFLAISSNTFALMGLISLFFALKGIMKLFRHL
                     KTGICFILFFVGAKMLLSGIESVSLFFAKHSWVSLTVILSTLAVSILYSVLETRMIEK
                     REAKLSANYEAAKAKKDDQHQAVDNFV"
     gene            4144..4542
                     /locus_tag="PKK03_07775"
     CDS             4144..4542
                     /locus_tag="PKK03_07775"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNX84339.1"
                     /translation="MANIEITLDGGKVVTAHINGKTIQTDQSVRNGGGGTAPEPFELF
                     LASIGTCAGIYVKSFCDQRGIAAEGIRIIQSVEFDPDKRVPSRFRLNIELPADFPEKY
                     RAAVINAAELCLVKKTINNSPQFEVTASIS"
     gene            complement(4535..5977)
                     /locus_tag="PKK03_07780"
     CDS             complement(4535..5977)
                     /locus_tag="PKK03_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNX84340.1"
                     /translation="MKVSVMFPARLVGMLCIAAILTLASGCQKETTEPADLKGDVPVM
                     LKSGQANEFIVISKSEILPAGLDRKLSAYGEIVSMTPEIGVMVVRPAASDSRDKISKL
                     PEVRAVVPDYNARWIEPAMNLSEATAESIGDNERFFSYQWNMRAISAPQAWDAGYTGE
                     GVRVFILDNGIDADNPDIRPNLNTQLSYSFVPDEPWYASEGSFFNHGTHVAGIVAGAD
                     NQWGIIGVAPHAELVAVKVLRESTGSGNFSWIFNGIVYSALNGADVINMSLGAWIDRA
                     GFFQDEEGNWHHMPAADYQNLIIAQQRAIDYAWRSGAVVIASAGNEGMNADGNRSLIK
                     IPGELNNVLAVSATAPECWWGQQNPFLDYPASYTDYGRSLIDIAAPGGDFDCDTYDMI
                     LSSAAGGPASYTFYFGAGTSQAAPHVAGVAALVIGKYDGNITPLEVTRILTLTADKIE
                     TNGQSLFYGKGRVNAWRAVTETIPGRGRFN"
     gene            6123..6638
                     /locus_tag="PKK03_07785"
     CDS             6123..6638
                     /locus_tag="PKK03_07785"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNX84341.1"
                     /translation="MTEKLKEVKPSDLTDNLIRLIASDWMLVTAGNRNKFNTMTANWG
                     GMGYLWNKNVVFVFVRPERYTYEFMESHEGFTLTFFDDRYRDALNLCGTKSGRDCDKV
                     AETGLTPFFTKSGLPAFAEARIVLECRKLYASQLTREAFLDNEPLKTHYSTKGGLHKI
                     YIAEIVNCLAR"
     gene            complement(6690..7685)
                     /locus_tag="PKK03_07790"
     CDS             complement(6690..7685)
                     /locus_tag="PKK03_07790"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84342.1"
                     /translation="MLRVLIVYDIDHTWDAAEISETRRSNRILFDALRKEGFETYLEE
                     LRDPHLDRILEKHDPRHTIVFNLCEALPGSSHPERRVTEIFENRGFTYTGNTPEVIDL
                     SYDKQKTKELLTTLGIRVPDGAVLSAEEAHTWTLFPAIVKPSREHCSLTISEKSVVFD
                     TASLKEQIRLVNSELNQPALVEDFIDGREFHVSVWNNGPPEILPVAEMDFSAFSEAKE
                     RLCTYDSKFLPGSCHYEKIETVMPAPLDDWLYKKLEKKTIDTWNGFGCTDYARFDFRL
                     RNDKFYLLDINPNNDISFDTSFAMAAGMMNYSYGQMVKRIVMMAAGRHPVFSDGF"
     gene            complement(7848..8615)
                     /locus_tag="PKK03_07795"
     CDS             complement(7848..8615)
                     /locus_tag="PKK03_07795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNX84343.1"
                     /translation="MLFLPESASLLHNEIISAILPVVLTILFFTMYWLVASSRRLKAK
                     LYGAYEPLRANSLHVVLKRSAGFVLLGVLPVAVMVLLNDNFTLAGAGVRFNPETTRFT
                     VISIILLSLLVIPIAFFSARKPDVYSIYPEIRAKCWTIGLLSAESFTWAVYLLGYETL
                     FRGVLLFGLVGTTGPVTATVINVILYSAAHLPKGKTETLAAIPFGIVLCILTLRSGTI
                     WIAFIVHLVNAVTATFTAISYNPEMRFAPGKARLSGF"
     gene            8881..9945
                     /locus_tag="PKK03_07800"
     CDS             8881..9945
                     /locus_tag="PKK03_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84344.1"
                     /translation="MKSRNSSFKIGIVALAVTALTACNQAPAKKEANTGTVHFRNLLF
                     SETPWDTERGSHELTPAQAKDINNYKFTYDDAGRLASVEFVRGDEPLGYGSLGGAAKI
                     TYEYTGDKQIKRFFDENGQPVESGGVFTYEYTLDEKGTRTALRFLDKDGNPVENRNKI
                     HNYIWRILPDGKLQEKRYNLAKEETVMNPFCPFYELRFTYNDKGYITEMANYMDDKLY
                     NCTAENCGDIGVSYFQFESNEHGDLLKFSVYNVTGQMSNLYWGWSRRASTYDENGYVL
                     ETAMFDQDNEYVAGKMIPVTQYTYDRHGSVTEVRNMDKDRNIINNPDNGVAITQYKYD
                     EKGNRTETLRFDRDRVAVAQ"
     gene            9965..11005
                     /locus_tag="PKK03_07805"
     CDS             9965..11005
                     /locus_tag="PKK03_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNX84345.1"
                     /translation="MKSRIFIGLMAVSLVALLAAGCAGKGTEKKKESTAPAEKAAPVI
                     DPETTLLLKDLEENGDYVNSQYFPSLIKASIVFEELDKNNLVIDLRPEEQFAAGHIKG
                     AVNRQFPGIPEYLEAGIKPFEYDKIILVCEDGQLSSYTTALLRMMGYGNVFAMRWGMG
                     GWNRQYAEAGWMKGLSAAYEDRLETTSNETPAAVGMPVPGTGLQTGSEISDARFRKLF
                     EKGAGNILISADEVYADPSKYFVINLERKDKYDAGHIPGAVRYKPEATLGFPDMMATI
                     PTDKITVVYCGTGHNSAFATAFLRLFGYDARTLRYGNNSFMYDRMVKDRNTLSWLPFT
                     LEESNDYPVVKK"
     gene            complement(11044..11454)
                     /locus_tag="PKK03_07810"
     CDS             complement(11044..11454)
                     /locus_tag="PKK03_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84346.1"
                     /translation="MKTLKKYRYVLAAVVVIAVMVLIRAFNPGSFRYDAAKWAEPSVT
                     GRNIVTPENLPEIGDNILFVMLDTECQAPDIPGADKLSVDPADLLSGEIIRKIRKSKG
                     PVVLCSEDVSISSRVWMALSEMGIRKLHILKNPV"
     gene            complement(11466..12077)
                     /locus_tag="PKK03_07815"
     CDS             complement(11466..12077)
                     /locus_tag="PKK03_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNX84347.1"
                     /translation="METRKQLTVLLLLLSLILALLPLTANRSFKGRPAALLEGVLQSE
                     TSFSADQVAGFIVNEAGDVQLFDLRPSVEYGQAFIPGAVSVPYAGFIRTDPDTWLRDR
                     NKKMILYSSDDTEAAAAMTYARGLGYDNTFFLRGGMEEWIKTVSSTEFTGERLTAREN
                     ALLETRRKAAGMYNELRSLPDSLKAKYLESKKFSARKLDGGCQ"
     gene            complement(12084..12689)
                     /locus_tag="PKK03_07820"
     CDS             complement(12084..12689)
                     /locus_tag="PKK03_07820"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNX84348.1"
                     /translation="MAPLSSLYQFPEWLNMLTALIIGIGFGFALEQGGFSSSRKLAGM
                     FYGYDTTVLKVFFTAAIVALIGSQFLGYFGLLNLNQVYVNEFYVTASIIGGVIMGGGF
                     IMGGFCPGTGLCAMSIGKIDAMVYILGGLTGAFLFAETYPMIEKMATANYRGPVKINE
                     ALGMSAGLFTLILVAAAAAMFWFAEKAEKKFARPDITNEIK"
     gene            complement(12890..13399)
                     /locus_tag="PKK03_07825"
     CDS             complement(12890..13399)
                     /locus_tag="PKK03_07825"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNX84349.1"
                     /translation="MKRKYMNPYLAGVLLGLVLLLAMVVAGRGLGASGGIKYCVVSIV
                     GAVSPEHVSQAEYYSGYFENGKKPLTNWLTIEIFGVLLGGFISGALSGRLKLKVEKSP
                     RITNTRRLVFAFLGGMLFVYGGQLARGCTSGAALSGMAVLSLAGFVTMIAIFGSAFAF
                     AWFFRKNWI"
     gene            13788..14147
                     /locus_tag="PKK03_07830"
     CDS             13788..14147
                     /locus_tag="PKK03_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84350.1"
                     /translation="MSATSMTVDQQEGLISMDLVRPSFDGGDMKTTEKYTLDGKESVN
                     TGMMDSSIKTITTWSDDKKELKFAKTILFDMNGDTMEIKISEIWSMSEDGKTLTVKST
                     MSSQMGESTVTLAYDKK"
     gene            complement(14180..>15374)
                     /locus_tag="PKK03_07835"
     CDS             complement(14180..>15374)
                     /locus_tag="PKK03_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNX84351.1"
                     /translation="LLHMKYLISIVGIALLMPFGLRAQQSNVNLDYNPQKDTEGLIPF
                     SASVNSPEVRDDNTVTFRLKAPNAQKVVLPVEAIHTSNNLGREPIPFTKGEDGVWTLT
                     IGPLKPDMYAYHFNVDGVQIPDPNNTYAAFTAMPPYSELIVHGDGPSYYDARNVPHGT
                     VTRHVYHSDVTGGERELYVYTPPGYNPKKKYPVLYLLGGSGELPSNWVFDGRANFILD
                     NLLAEGKAVPMIIAIPNNQVIHRNHPKHTELTFDIFEKELRNHVIPLVDEAYSTIRSP
                     KGRAISGLSMGGRHSMFIGFRSLDLFASFGILSAGDTNAETTLEQFLNDPKVNEKIEY
                     LFVGQGTEEAKGFMGQRCLVLHEALLNHGIKHQYYVGGHGGHDWSTWRHLLYYEFLPN
                     LWKGR"
BASE COUNT         4034 a         3930 c         3660 g         3750 t
ORIGIN      
        1 cagaccttca gaccttgtga ccttcagacc ttcagacctt cagaccttca gaccttcaga
       61 ccttcagacc tatatctacc accctatccc gtagtcctcg ccgtgattgc tggatccgcc
      121 ccagaaggtt ccgtgcgccc tgtcgaatcc gatggcattg acgggaccgg aggttctctc
      181 ctggaacgat agcctgtacc ccatggctgc cagttctttt cttacccacg aaggcatggc
      241 gctgttcagc accaatccgc cgggaacctt ctcatgctcg ccgaagctgg aatacatctg
      301 cagcgtcttg aatgacggcg cctcgcatgc ttcctgaacg gtcatgccaa actccaccac
      361 attcagaaag aactgaagca gcaactggtc ctgctcatcg ccgccctgct tggcaaacga
      421 tagcagcggc ttcccgtcct tcatcgccag cgtgggagtc agggtcacac gcggtctctt
      481 gccgggttca accacgttga acgggttctc cttcgcgtca aggacgaaac tctgcatcct
      541 ctggctcatc ccgatgccgg tatttcctgc aatgcatgcc ggcacccacc cgccgctcgg
      601 agttacggag acaacccatc cctcctcgtc agcggcctcg atggaggttg tacccgactg
      661 aaatttactc atgaaactgc tgtactgctc cggatcataa gatggataaa tattacgggc
      721 atctgcctgc catgaatcca gcactgtgga gaaaggattc accttcccct cgaagagata
      781 gggatctccc ggcctgacag ccggatcatt gcggtcagga ttgataagct tgctccgctc
      841 ccttgcataa tctttggaga gaagtccctg catcggctct tcgggcggga aggacgggtc
      901 accgtaataa aagtccctgt cggcaaaagc caggttcatt gcctggtaaa gtgtatggat
      961 gtaccttgtg gagttgtagc ccaactcctt caggttgaag ttttcaagta tgttcagggt
     1021 ctggagcatc accggaccct gggtccactg ctgcagcttg tacacttcaa tgcccttgta
     1081 ctctgtcata agcggttcct caatcttaac ctgccagccg gccaggtctt cctttgttat
     1141 cagtccgccc tgttcctggc atccacgtgc aaactcggct gcaatgtctc cgttatagaa
     1201 acggtcatag gcagcataga tggcatcacg cctcgatttc ttcttcttca gtgcctgctg
     1261 ttcggcctcg accagctttg tcagggtagc aagaaggtcc ttctggacga agacttcccc
     1321 ggctgagggt gcctcccgtt cttccccgcc atgcgggaga aacaccctcg cagaataggg
     1381 ccaatccttt atcctgttct ttgcacgctc gatggaattg gctgtctggg cctccatcgg
     1441 gtagcctgct gccatctcca tggccggggc caaaacctct ctcaggctca tggtgccgta
     1501 ctccgccagc atcaccatca gtccaccggg tgtacccgga gttactgcgg caagcggacc
     1561 atattcaggc gggtatttgt aacccttcga tttgtagaat tccgctgtcg cacctgtcgg
     1621 ggccactcca agggcattga ttgccagcac cttcccggtc ttcggattat agataagcgc
     1681 ctgggtctcg cctccccagc taagtacgtc ccacattgtg catgaggctg ccagcatcgc
     1741 gcatgcggca tccactgcgt tacctccgcg atcaaatatc atagcccccg ccgtcgcggc
     1801 aagaggtttg ccggtgatgg ccatccagtg ccggccatgc agcggcggct tctgggtggt
     1861 aaccggaaaa gtgttaaatg actgtgcata tgacatgatg aaaatgctta aaagactgat
     1921 tgataaaaaa aatttcctca taatggccag cattgtttcg gggaaggaac aggctccctc
     1981 ccggaatgca atttcgcaaa taatcgcatt gatgatacga aaaagtgatg atttggctcc
     2041 gcaggcaggt caggataact gttccctgcc cacagtacgg ggattgagaa atacaaggaa
     2101 gaatatgact gagaaagcaa gctcgttcag ctcgctccct tttgccatcc tgatgactgc
     2161 tatcagtgcc gttgatactc ccataatgat tatatgacgg agcgagggca gcggcaatcc
     2221 gaacttctct acgagcctcc ggatcaggcc cggtttcttt gcaagcaccg gaagaatgat
     2281 aaaataaaag agtaatgcaa tcaggaccat tctggaaaag atgatcatat tgatgctttt
     2341 gccggcgaca accaggttgt gaatattggt ttccgcctgc ttgttgtact tcagaaagaa
     2401 ttcactgctc tcgatgttga agatacgctg gccccagctg atctcatcac cggcagcaaa
     2461 aaagaaaagg aaggagagga ctgaccatgt cagtacgggg agatgccgtt tccccctgca
     2521 acaggtgaaa gccctgaaga tggcaaccag gcttcccgca aaaagaaaaa acgcagtggc
     2581 atattctgca gggccatcct ccctcacata atacctgaag gcatccggtc ccccgagata
     2641 tctgacacca atgccgtaca gcactgaaaa caggagtgct gcagccaagc caatctcaat
     2701 caaacctgtt ggtttctccc tttccataaa atcctgacaa aactgtaaac agacgtattg
     2761 cgatatgcaa atatatacaa gttaccttac ctgagcaacc gctgaccggc taagggagtc
     2821 acttttgcgg catttcacgc cctaaccatg acaaatgttc tcagttttat catgacaaca
     2881 gcatacttta gcgcatcaaa ataaccatca caatgaatcc cggattatat ttctggtttg
     2941 cctttattct gattttcatc gtaggatttt gggtggatat gcgcgcggct aagaacagga
     3001 agtcggaaga gagcgttgcc actgcactca gatggaccgg tttctggatt accgtagcat
     3061 tccttttcgg actggccata ttcctgttct atccgcagaa cgaaggcagc gccatcagca
     3121 cgaggaacat tatgggaatg aaattctatt ccgcctacct cactgaatat atgctttcaa
     3181 tagacaacct attcgtcttc atcatgatct tcaccatgat gaaggtgccg ggtgacatac
     3241 aacctattct gctgaagtac ggcatattgc tttcgatatt cttccgtacc ctcttcatac
     3301 ttgtgggaat ggggctcata aaccggtttg agtggctgat ctatcttttc ggcgcattcc
     3361 ttatctacat gacattcaaa atgatgaaga gcagcgatga ggaagataca attgatccgg
     3421 agaaaaatac acttatacgt gcagcaaaga aagtattcca ggtagacgtc aacaacaaga
     3481 accccagatt ccattcccgt ttaaacggca agtttgcaat tacccccttc gccctgacat
     3541 tcctgctgat aggatcaact gatgtcatgt ttgccgttga ttccgttccg gctgtgatag
     3601 gggttatccg tgagggaaac caggcactta cactttatga aagcaacttt ctggcaatct
     3661 catccaatac atttgccctg atgggtctga tctcactgtt ctttgcgctc aagggaataa
     3721 tgaaactgtt caggcacctc aagacaggaa tctgcttcat ccttttcttc gtgggagcga
     3781 aaatgcttct cagcggcatc gaatcagtca gcctgttttt tgcaaaacac tcatgggtat
     3841 ctttgacagt gatcctttca accctggcag tatcaattct ttattccgtg ctggagacaa
     3901 ggatgattga aaaacgtgag gcaaagcttt cggctaacta tgaagccgcc aaagccaaaa
     3961 aagatgatca gcaccaggca gttgacaatt tcgtctgaaa tgatccggga acaggggagg
     4021 atatttctgc ggtaaatcat atctttcctc cacctgattc agcaatattc aaacaaaata
     4081 aggcttcatc tgttgaggag ctgagaaaag cagcagatca agcggcaaaa atattataat
     4141 catatggcaa acattgaaat tactctcgac ggcggtaagg tggtcaccgc ccacataaac
     4201 ggcaaaacga ttcaaaccga ccaatctgta aggaacggcg gcggcggaac tgcccccgag
     4261 ccgtttgagc tgttccttgc atcaatcggg acctgtgcgg gaatctacgt aaaatcattc
     4321 tgtgaccaga ggggaattgc agccgaagga atcagaataa tccagtcggt tgagtttgac
     4381 cctgacaaga gggtaccctc ccggttcagg ctgaatatcg agcttcccgc ggattttccc
     4441 gaaaagtacc gtgcagccgt aatcaatgcg gcagagttgt gtctggtcaa aaagaccatc
     4501 aacaactcgc cgcagttcga ggtgacagct tcaatcagtt gaagcgacct cttcccggga
     4561 tggtctccgt aactgccctc caggcattca cccttccttt tccgtaaaag agggattggc
     4621 cgtttgtttc aatcttgtca gcagtcaggg tcagaatcct agttacttca agcggggtga
     4681 tatttccgtc gtacttcccg atcaccaggg cagccactcc tgccacatgg ggagcagcct
     4741 ggctggtgcc agcgccgaaa tagaatgtat atgaggccgg tccgccggca gctgagctta
     4801 gaatcatgtc atatgtgtcg cagtcaaaat cgcctcccgg agctgcgata tctatgagtg
     4861 agcgtccgta atcggtatag cttgccggat aatccaggaa aggattctgc tgaccccacc
     4921 agcactcagg tgccgttgct gataccgcaa ggacgttgtt aagttcaccg ggaattttta
     4981 tcagagaacg gttgccatct gcattcatac cctcattccc tgcggaggct ataactactg
     5041 cgccactcct ccatgcatag tcaattgcac gctgctgggc gatgatcagg ttctgatagt
     5101 cggctgcagg catatgatgc cagttgcctt cttcatcctg gaagaatccg gccctgtcaa
     5161 tccatgcacc aaggctcatg ttgattacat cggcgccatt gagcgcggaa tatacaatcc
     5221 cgttgaagat ccagctgaaa ttacctgaac ctgttgattc gcggagcact ttgacagcca
     5281 ctagctcagc atgcggtgca accccgataa taccccactg gttgtctgcc ccggcaacta
     5341 tcccggccac atgtgtaccg tggttgaaaa atgaaccttc actggcatac cagggctcat
     5401 cgggaacgaa ggagtagctc agttgagtat tcaggtttgg ccggatatcg ggattatcag
     5461 catcaatacc gttatcaagg atgaatactc ttactccttc tcctgtgtag cctgcatccc
     5521 atgcctgggg tgcactgatg gctctcatat tccactgata gctgaaaaat cgttcgttat
     5581 cgccgatact ttctgccgtg gcttctgata aattcattgc aggctcaatc cacctggcat
     5641 tgtaatcagg aacaacagcc cttacctccg gaagttttga aatcttatcc cttgaatccg
     5701 aagcagccgg cctgacaacc attaccccga tttcaggagt catgctcaca atttccccgt
     5761 aggctgacag ctttctgtca aggccggcag gaaggatttc actcttcgat attacaatga
     5821 attcatttgc ctggccactc tttagcataa ccggaacgtc acccttcagg tctgccggtt
     5881 ccgtggtttc cttctggcat cccgaagcca gggtcaggat tgctgctatg cacagcatcc
     5941 ccactaatcg ggctggaaac ataactgaga ctttcatttc taaattattt ttaattaagg
     6001 ttaattgatg ctctaaatct aaacaatttt tttatcaaag gggcgtaacg ttcatttaaa
     6061 tgagaggaaa gtaatgaacc ggttatcaac aattgttata ttagccggaa atttttcgtt
     6121 taatgacaga gaagctgaag gaagtgaaac cgtcagacct tactgacaac ctgatcaggc
     6181 tcatcgcatc tgactggatg ctggtgacag ccggcaacag gaacaaattc aacaccatga
     6241 ctgccaactg gggagggatg ggttacctgt ggaacaaaaa cgtggttttc gttttcgtaa
     6301 ggccggaaag gtatacctat gaattcatgg agagtcacga gggtttcacc ctgactttct
     6361 ttgacgacag ataccgggat gcccttaacc tttgcggcac aaaaagcgga agggattgtg
     6421 acaaggtggc agaaacagga ttgacgccat tctttaccaa atcaggcctg ccggcatttg
     6481 ccgaagcaag aattgttctg gagtgccgta agttgtacgc cagccagctg acaagggagg
     6541 cattccttga caacgaaccc ctgaaaaccc attacagcac caaaggcgga cttcacaaaa
     6601 tttacatcgc tgagatagtg aactgcctgg ccagatagga aaccgggcaa ctgttttgca
     6661 aaccggtcct ggcagctgta cctgccggtt cagaatccgt cactaaaaac agggtgccgc
     6721 ccggctgcca tcatcactat tctttttacc atctgaccat aggagtaatt catcatcccc
     6781 gctgccatcg caaagcttgt gtcaaagctt atatcattgt tgggattgat gtcgagaagg
     6841 taaaacttgt cattacgcag ccggaagtca aaccgtgcat aatccgtgca cccgaatccg
     6901 ttccaggtat caatggtctt tttttccagc tttttataca gccagtcatc gagaggcgca
     6961 ggcattaccg tctctatctt ttcatagtgg catgagccgg gaagaaattt tgaatcatac
     7021 gtacatagcc tctccttcgc ttcgctgaag gccgaaaagt ccatctcagc cacaggcagt
     7081 atctcaggcg gaccattatt ccagacagag acatggaatt cgcggccgtc aataaaatcc
     7141 tccaccaggg caggctgatt cagttcactg tttacaagcc ttatctgttc cttcagcgag
     7201 gctgtgtcaa aaacaacgga tttctctgaa atagtcaggc tgcagtgctc acgcgagggt
     7261 ttcactatgg ccggaaagag agtccaggta tgtgcctctt cagcgctcag aaccgctccg
     7321 tcaggaaccc taatccccag cgtcgtgaga agctcctttg tcttctgctt gtcataactc
     7381 aggtcgatga cttccggggt gttcccggta taggtgaacc cccggttctc aaatatctcc
     7441 gtcactctcc tttcgggatg cgaggaaccc ggaagggctt cgcagagatt gaagacaatt
     7501 gtgtgccggg gatcatgctt ttccagtatc ctgtcgagat gagggtcacg cagttcttcg
     7561 agataggtct caaaaccctc tttcctgagt gcgtcaaaga gaatgcggtt cgatcttcgc
     7621 gtctcgctga tctcggccgc atcccacgta tgatcaatat catatactat aagaaccctc
     7681 aacaaaaaag ctttgttacg aaactaaaat aaatgttcga aaaatggaga aatttcaatg
     7741 aaaaaatgca ccttttttcg tcaaaaaaat catgccccca ggaatacttt tctttaatcc
     7801 cggaatatca ggattttcag gattcggtga ctgcctttac cttcccgtca gaaccccgat
     7861 aatcttgctt ttccaggggc aaatctcatc tcgggattat agctgatcgc agtgaatgtt
     7921 gcagtcactg cattgaccag gtgcacgatg aatgcaatcc agatggttcc tgaccggaga
     7981 gtcagtatgc ataggacaat tccaaacgga attgctgcca gcgtctccgt cttacctttc
     8041 ggcaggtgtg cagcagaata caatatgaca ttgattacag ttgctgtaac aggacctgtg
     8101 gtaccgacaa ggccgaagag cagaacacca cggaaaagcg tctcatagcc caggagatat
     8161 acagcccatg taaatgactc ggcagacagg agaccaatcg tccagcactt tgcccgaatt
     8221 tcagggtaaa ttgaataaac atccggcttc cgggcactga aaaaggctat cggaatgacc
     8281 agcagggaaa gaaggattat gctgataacg gtaaaacggg tggtctccgg attgaacctg
     8341 accccggcac cggccagtgt aaaattgtca ttgagcaaaa ccatcacagc cacgggcagc
     8401 actccaagca gcacgaatcc agccgacctt ttcagcacca catgaaggct atttgccctg
     8461 agtggctcat acgccccgta caattttgcc ttcagcctcc gggaacttgc caccagccag
     8521 tacatggtga agaataaaat tgtcagcaca accggcagaa ttgcggatat tatctcatta
     8581 tgaagcagtg atgctgactc aggtaaaaat agcattgact gtaaagtaat tgttctgtta
     8641 aaaattcttc cctgcctctc ggcaggaacg gagatttgca acacatcccc ggtcatccaa
     8701 aaataaggaa aagttgctgc aggggaacaa caggtctgaa aatccgaagg aatcaggcaa
     8761 aatttgatcc gggtgctctc cggcatcttt atctgcttgc tccactccga ttttagtcat
     8821 aaattgcgga aaccttttta ccctgacatt gttctattgg tatcaaacaa aaaagaaagt
     8881 atgaaatcaa gaaactcatc gtttaagatt ggcattgtgg cactggcagt tacggctttg
     8941 acagcatgca accaggcacc cgcaaaaaag gaagccaaca ccggaaccgt tcatttcaga
     9001 aacctccttt tcagcgagac accgtgggat accgaaagag gatcgcatga gctgactccg
     9061 gcacaggcaa aggatatcaa caactacaaa ttcacttatg acgacgccgg ccgccttgca
     9121 tcggtagaat tcgtaagggg cgacgagccg cttggctatg gatccctcgg cggagccgcg
     9181 aaaatcacct atgaatatac aggcgacaaa caaatcaagc gcttctttga cgaaaacggc
     9241 cagcccgttg aatcaggagg agtctttaca tatgagtata ccctcgatga aaagggaacc
     9301 aggacggcac tcaggttcct cgataaagac ggaaacccgg ttgagaacag aaataagatc
     9361 cataactaca tctggagaat cctacctgac ggcaaactgc aggagaaacg ttacaatctt
     9421 gccaaagagg aaaccgtcat gaacccattc tgcccgttct atgagctcag gttcacttac
     9481 aacgacaagg gctatatcac cgagatggcc aactacatgg atgataaact ctacaactgt
     9541 accgcagaaa actgcggtga cattggagta tcatacttcc agttcgaatc gaatgaacac
     9601 ggcgacctgc ttaaattctc agtctacaac gtgaccggac agatgtctaa cctgtactgg
     9661 ggctggtcgc gccgcgcaag cacatatgac gaaaacggct acgtgctcga aacggctatg
     9721 tttgaccagg acaatgaata cgtcgccggc aaaatgattc ccgtcaccca gtacacctat
     9781 gacaggcacg gctcagtgac cgaggtcagg aatatggaca aggacaggaa cataatcaac
     9841 aatcctgata acggtgttgc aatcacccag tacaaatatg acgaaaaggg gaaccgcacc
     9901 gagaccctga ggtttgacag ggaccgggtg gcagttgccc agtaatgctc ataccgcttc
     9961 aaatatgaaa agcaggatat ttatcgggct tatggctgta tcgcttgtcg cactcctggc
    10021 tgcaggatgt gcaggcaagg ggaccgaaaa gaaaaaagag agcacagcac cggcagaaaa
    10081 ggcagcaccg gtaattgacc cggagacaac gcttctgctg aaggacctcg aggagaacgg
    10141 cgattatgtc aacagccagt acttcccgag ccttatcaag gcgtcgatcg tctttgaaga
    10201 gctggataaa aacaacctgg tcattgacct ccggccggag gaacagttcg ccgcagggca
    10261 cataaaaggg gctgttaaca ggcagttccc agggatcccg gaatacctcg aggcgggcat
    10321 aaagcctttt gaatatgaca agataatcct cgtctgcgaa gacggtcagc tctccagcta
    10381 taccaccgcc cttctcagga tgatgggata tggtaatgtg ttcgcaatgc gatggggtat
    10441 gggaggctgg aacaggcagt acgccgaagc cggatggatg aaggggttat ccgcggcata
    10501 cgaggaccgg ctggagacca catccaatga gactccggct gcagttggaa tgcctgtacc
    10561 gggaaccgga cttcagaccg gaagtgagat cagtgatgca aggttcagga aactgttcga
    10621 aaagggagcg gggaatattc tcatctctgc agatgaagtg tacgctgatc cttcgaagta
    10681 ctttgtgata aacctggaac gcaaggacaa atacgatgcc gggcacattc ccggcgcggt
    10741 aaggtacaaa cccgaggcaa cacttggttt tccggatatg atggcaacca tacccaccga
    10801 caaaatcaca gtcgtctact gcggcacagg ccataactcg gcttttgcaa cagcattcct
    10861 gagactcttc ggctatgatg cccgtaccct cagatacgga aacaacagtt tcatgtatga
    10921 ccggatggtg aaggacagaa acaccttgtc atggcttcct ttcacgctgg aggaatcaaa
    10981 tgattatccc gtggtgaaaa aatagttcct ccgaacttca gaaatactaa tccggccagg
    11041 taatcagacc ggatttttta atatgtggag cttcctgatt cccatttcac ttagcgccat
    11101 ccagacacgt gaagatattg aaacgtcttc tgagcaaaga actacagggc ccttgctctt
    11161 tctgatctta cgaatgatct cacctgaaag caggtcagcg ggatcaactg aaagtttgtc
    11221 cgcaccgggt atgtcaggtg cctggcattc agtgtcaagc ataacaaaaa gaatgttgtc
    11281 accgatttcc ggaaggtttt caggagtgac tatattcctg cctgtgaccg acggctccgc
    11341 ccactttgcg gcatcatacc tgaaggagcc gggattaaaa gcgcggatca aaaccatcac
    11401 tgcaatcacc acaaccgcag ccagtacata tctgtatttt ttcagtgtct tcatgatgct
    11461 aataattact ggcaaccgcc gtcaagtttc cgtgctgaaa atttctttga ttcaagatat
    11521 tttgctttca gactgtcagg gaggctgcga agctcgttgt acatgccggc tgccttccgc
    11581 cgtgtctcca gcagcgcatt ctccctggcg gtgagccttt cgccggtgaa ctcagtggaa
    11641 gagactgttt taatccattc ttccattcct cccctgagaa aaaaggtgtt gtcataaccc
    11701 aggcccctgg catatgtcat ggctgctgcc gcttccgtat catccgatga atacaggatc
    11761 atttttttgt tcctgtcgcg cagccaggta tccggatctg tccggatgaa gccggcatac
    11821 ggcacgctga ctgcaccggg gatgaatgcc tgcccgtact caacggaagg tctgaggtcg
    11881 aagagctgaa catctcctgc ctcattcacg atgaatccgg ccacctggtc ggctgaaaat
    11941 gacgtttcgc tctgcagtac cccttccagc agtgcggccg gccggccctt gaacgaccgg
    12001 tttgccgtca gcggaagcag ggccagaata aggctcagga gaagaagcag tactgttaat
    12061 tgtttcctgg tttccatggc aggtcatttt atttcattgg tgatatcagg tctggcaaat
    12121 ttcttttcgg ctttttcggc aaaccagaac attgccgcgg cagcggccac aagtatcagg
    12181 gtaaagaggc cggctgacat gccgagagct tcgttgatct tcaccggccc ccggtaattg
    12241 gccgtggcca tcttctcaat catagggtag gtctcggcga acaggaaagc tcctgtgaga
    12301 cccccgagta tgtagaccat ggcgtcaatt ttaccgattg acatggcgca aagccctgtt
    12361 cccgggcaga agccgcccat tatgaagccg ccgcccatta tgactccgcc aattattgag
    12421 gcagtgacgt agaactcatt gacatatacc tggttaagat tcagcagccc gaagtacccg
    12481 aggaactgtg aaccgatgag ggcaactatt gctgcagtga aaaatacctt cagcactgtg
    12541 gtgtcatacc cgtagaacat gccggccagc ttgcggctcg atgagaatcc tccctgttcg
    12601 agggcaaatc cgaatccgat gcctataatt aacgctgtca gcatgttcag ccattcggga
    12661 aactgatata atgatgatag gggagccatt gtgatttgcg atttgagagt tgcgatttgc
    12721 gatttgagag ttgagagttg agagttgaga gttgcgattt atgttgcgcg agttgttgta
    12781 ttaaatccat ctttatccag accttcagat ctcagattga ggaaccgccg ccggcgggac
    12841 cgaaatcccg atacccgaag tgtgccggga ccttcagacc tcagaccttt catatccagt
    12901 tcttcctgaa aaaccaggcg aatgcgaatg ccgagccgaa tatcgcaatc atggtcacga
    12961 atccggcaag ggagagaacg gccatccccg aaagggcggc gccgctggtg catccccgtg
    13021 cgagctggcc tccgtagacg aacaacatgc ctccgaggaa ggcaaataca agccttcgtg
    13081 tgtttgtaat ccgcggagac ttctccactt tcagtttcag tcttccggag agggctcctg
    13141 agatgaatcc gcccagaagc acgccgaata tttcgattgt cagccagttg gtcagtggct
    13201 tcttcccgtt ctcaaaatac cctgaatagt attctgcctg gcttacatgt tccgggctga
    13261 ctgccccgac aattgagaca acgcagtatt tgatcccgcc gctcgctccc agtccccttc
    13321 cggcgaccac catagccagc agcagtacaa gtcccagcag tactcccgcc aggtacggat
    13381 tcatatattt ccttttcata gttccggttt atgaataaac aaggtacagc aaaaataagt
    13441 ttattgccgg aaggattgca ggaaacccga cagaatgtgc gatatctgcc ttaagtgttg
    13501 ttttttttaa atgcaacatg aaaaccacaa gtaaaatatt tatgtatttt taggatcaac
    13561 aaattgttca aataccagta tcatatttct aataatcttt aataaaaatc attatgaata
    13621 atcaaaagat ttctacttcc ggcaaacgat tctttaacat ttcgttgcct cttttatttg
    13681 catttgtatc cttaggtctt gtaccgggga gtttatttgc ccaaccgaat ttttcaggtt
    13741 catgggcatt caatgaatca aagagtattc ttggtgacgg accaatgatg tctgcaacat
    13801 caatgaccgt tgatcagcag gaaggcctaa tctccatgga ccttgtccgg ccttcgtttg
    13861 acggcgggga tatgaagacg actgaaaaat atactcttga cggcaaggaa tcagtcaaca
    13921 cgggtatgat ggactcttcc attaaaacca tcacaacctg gtcggatgac aaaaaggaac
    13981 tcaaattcgc caaaacaatc ctctttgaca tgaacggtga cacaatggag atcaaaatct
    14041 cagaaatatg gagcatgtct gaggatggaa agactctcac tgtgaaatca accatgtcaa
    14101 gccagatggg agaatcaacc gtgacactgg cttacgacaa gaaataattc tcttttcctt
    14161 agtttcaaga ttatttgagt tacctgccct tccaaaggtt gggcaggaac tcataataaa
    14221 gcaggtgccg ccaggtcgac caatcatgac ctccatggcc gccaacataa tactgatgct
    14281 tgatgccatg gtttaaaagg gcttcatgca agaccaggca cctttgcccc ataaaacctt
    14341 ttgcttcctc ggttccctga ccaacaaaaa ggtactcaat cttttcattt actttcggat
    14401 catttaagaa ctgttccagc gtggtttccg catttgtatc ccctgcgctg agaatcccga
    14461 aactggcaaa caggtcgagc gaacgaaacc cgataaacat actgtgacgg cctcccatcg
    14521 ataaacctga aatggccctg cctttgggcg acctgattgt gctgtaggct tcatcaacca
    14581 gcgggattac atgattccgg agctcctttt cgaaaatatc gaaagtcagt tcggtatgtt
    14641 tgggatgatt ccggtgaatt acctggttgt tggggatcgc aatgatcatc ggaacagcct
    14701 tcccttcggc aagcaggttg tccagaataa aattcgccct tccgtcgaac acccagttgg
    14761 aaggaagttc accgcttccg cccagcaaat acaaaaccgg gtattttttc ttcgggttat
    14821 aaccgggagg tgtgtaaacg tagagttcgc gctcaccgcc ggtgacatct gaatgataaa
    14881 catgacgcgt cactgtcccg tgaggaacat tccgggcatc gtaatacgat ggtccgtcgc
    14941 cgtgtactat cagttcactg taaggcggca tggctgtaaa ggcagcataa gtattgttgg
    15001 gatcggggat ttgaacgcca tcgacattga aatggtaagc atacatgtcc ggtttgagcg
    15061 gccctattgt gagtgtccag acaccatcct caccctttgt aaaagggatc ggctccctgc
    15121 caaggttatt ggatgtgtgg attgcttcga ccggcaggac aaccttctgt gcattgggtg
    15181 ccttcagccg gaaagtaacc gtgttgtcat cgcgcacctc gggagaattt acagacgcgc
    15241 tgaacggaat caggccttct gtgtccttct gcggattgta atcgaggttc acgttagact
    15301 gctgcgccct taggccgaac ggcattaaca gagcaatccc gacgatcgat atcaaatatt
    15361 tcatatgcag caaa
//
LOCUS       DAOPXU010000065        15198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_4235_length_15198_cov_12.051971, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000065 DAOPXU010000000
VERSION     DAOPXU010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15198
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4235_length_15198_cov_12.051971"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..1371)
                     /locus_tag="PKK03_07840"
     CDS             complement(157..1371)
                     /locus_tag="PKK03_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNX84352.1"
                     /translation="MSRYLALIIILLPMAVQAQITAPGSRTVRPTNYPATPRTDSVYI
                     FCATGPGDTGTLTASSPGGTAPFTFTWTRYNQSGGGYTIPVKTESGITSTATGLAEGG
                     YRVQITGDGGYSNEFYAWVNLSMPVAGAALQHYTCDYVALDGTKAADPFTYYDPLTNV
                     SRLLPNGVKHLWSSSPASAIPYPDIEIDPITFSPPLTDVRYTLQVTDSFGCSTSASFD
                     YTSIHVKAEFTADPVEGESPLEITFTDKSVRASKYLWKFGDDSVSTMQDPGTHTYYIP
                     GEYTVSLVIESELTCRDSATAKIEVLPSALQIPNVFTPNDDGLNDYFVPDKKSIRFLN
                     LQIFAKSGHRVYYYQGDGEDLQNWLGWDGRINYSERRAEPGAYYYVLRAEGYDDVEYK
                     GREFRGTVYLYR"
     gene            1462..3825
                     /locus_tag="PKK03_07845"
     CDS             1462..3825
                     /locus_tag="PKK03_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNX84353.1"
                     /translation="MFRDYLKELNEAQQAAVVNTSGPAMVIAGAGSGKTRVLTYRIAY
                     LLQQGVQAHRILALTFTNKAAGEMKERINRIVGPEKARYLWMGTFHSIFAKFLRYDGH
                     HLGYKSNFTIYDQDNTRSLIRTLIKEMNLDDSVYKPASVAGRISHAKNNLITAQQYSS
                     VPQITESDKASRVPEMAAIYRHYAARCFKANAMDFDDLLMNINILFRDFPAVLEEYRN
                     RFDYVLVDEYQDTNYAQYIIVHKLSENHRNLCVVGDDAQSIYSFRGARIENILNFKND
                     YPDYQLFKLEQNYRSTQTIVNAANSVIEKNAGQIRKTIFSKNEEGEKIRVIQTTIDSE
                     EGILTASDILDTRMRERLDWKDFAILYRTNAQSRIFEEMLRKRNIPYKIYGGQSFYER
                     KEIRDLIAYLRLVVNPDDEEALRRIINYPRRGIGDTTVMKLMDLARSLDLSIWSIITS
                     QELSAKYLAPALRTKISYFTGLMMPVMAVAETATAYDIAFNMASAGGILNELKQGQVP
                     EEIDRLENVQELLNGIKEFTESAVTNGEPADIATWLQSVSLLTDQDMEKPEDRDKVTL
                     MTIHSAKGLEFKYVYIVGLEENLFPSAMNLFNPRELEEERRLFYVAITRACRRVTLSY
                     ALNRYKWGTLERSSPSRFIGEIESEFLSYPQTGGKPFGQRAVYEPGPAGIIPEQSRPF
                     SPPARMTRLRDAKTPPTADGVNGAGPAGSGATGHSAGRSTGMGGGVIGNGINMGLEPG
                     DRVSHERFGEGEVVSVEGESPNTTALINFKTAGTKKLLLRFAKLTKL"
     gene            complement(4001..4777)
                     /locus_tag="PKK03_07850"
     CDS             complement(4001..4777)
                     /locus_tag="PKK03_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84354.1"
                     /translation="MEAKGIYRYVPSFSGSYGTGWRAMSDNFLRLLLVVLVLAIIGSP
                     MLGSHSKWEFNPGDFGNIPFDMHDFFKFGAFGLAAAFLGLIVLLYIFLLLPVFRYGAK
                     MMFVQASRQITPDFDLLISGFRKNYLNIVLTNLLLTALIGIGLVFLIVPGIILACRLT
                     FTPYLVMDKGLDPIRAAEESWRLTRGHGWAIFLMGFIAFFIYIAGFICFFIGVLVSDM
                     WVKSSFATLYHSVLIEKGMWQVEPVPAAEPVQGDAAANEA"
     gene            5688..5897
                     /locus_tag="PKK03_07855"
                     /pseudo
     CDS             5688..5897
                     /locus_tag="PKK03_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
     gene            complement(5894..6769)
                     /locus_tag="PKK03_07860"
     CDS             complement(5894..6769)
                     /locus_tag="PKK03_07860"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNX84355.1"
                     /translation="MAPKDSAEAKDIKCSMAGYIACSMDLLAMQPVPDDTAIHDVRVL
                     MKKHRAAVKLVKPMLDEAVYRREYLAGRETGRLLALWRETAVLRKTVKALKKDNPELF
                     IRLGGNEKIQELLRKPYSTWEQAGDRAKSVHEVASRLKKTQYRLRFLGLNEPDMRLML
                     GELGRSHATASAAYLACRNKPAARLLHEFRKKSKTFMYQLIFFRHLAPQTVRQLEKRL
                     GVMTQNLGKYNDLAQIMLLTGYRYGDPENSDMDNELAIVIRDRQDRYLEKVWPYAYRI
                     FAPGKKLQDILGISF"
     gene            6929..7981
                     /locus_tag="PKK03_07865"
     CDS             6929..7981
                     /locus_tag="PKK03_07865"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNX84356.1"
                     /translation="MGKSNSRDSQPRTIGILTAGGDCPGINAAIRGVGKTAIVKYGMK
                     VIGISDGYAGLINKEYRELTESDLSGILTLGGTILGTSREKPFKGTGKKKDETRKPLL
                     IKEHYEQMGLDCLVCIGGNGTMKTANMLSEEGLNVVGIPKTIDNDIWGTDMTFGFDSA
                     VNIATEAIDRLHSTANSHKRIMVIEIMGHNAGWLALYSGMAGGGDVILIPEIPHNEKV
                     VMDYLRKRADNNKPYSIVVVAEGIAIPEGEISPARYLARRIMEETGLETRETVLGYIQ
                     RGGSPSPMDRVLASRFGAAAANLIAKGEFGKMVAQRNGEISSVKLSEVGGKTKLVGKD
                     DPMVRQAREMGTCFGD"
     gene            8079..8609
                     /locus_tag="PKK03_07870"
     CDS             8079..8609
                     /locus_tag="PKK03_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNX84357.1"
                     /translation="MVKKSKTEESGTRKLILIRHSKAEDFSSEISDFERSLTVRGKIN
                     SRLMAEILKAKGEEPGRVISSPAFRALETALIFCREYDISPAEIRLAPELYHGLRESE
                     FLPFIQQQSDNDHTVTLFGHNPLITELAAYFASDEPEILPKTGIYCIEFQAAGWQDVE
                     PESGRTLYFLTPRELA"
     gene            8606..10660
                     /gene="ppk1"
                     /locus_tag="PKK03_07875"
     CDS             8606..10660
                     /gene="ppk1"
                     /locus_tag="PKK03_07875"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNX84358.1"
                     /translation="MSKAYIPKEISWLSFNGRVLQEAANSEVPLLERFNFLGIYSNNL
                     DEYFRVRVATLRRLTLYGSKAKGILGYSPKATLKKIQEIVLSQNDVFEKTYTGLLAEL
                     KKHRINFVNEQQLDPEQTEFVRSFFRKEVRSRLMPLIISKENKLPDLPDDAVFFAVQL
                     RNTAKDRKIYALLEIPPKEILPRFIMLPGRKGSRYVIFLDDVIRWGLHEIFSTLPFDE
                     LSAYTFKVTKDAELEIADDISESYIEKISKSLQLRRKGNPVRFVHDREMPQELLKILS
                     KKLNLGTDDVIMSGNRYHNFKDFMRFIEIEGSGLSYPKLPPVRHPALHYGKSILSAVR
                     KRDILFYFPYHPFEHFIDLLREASIDPYVTSVHITLYRLARNSSVINALMNAARNGKS
                     VTTVVELQARFDEEANILWGNKLQDEGVKVIYGVPGLKVHSKLCLITREKEGVVQRYA
                     VVGTGNFNEETARVYTDHLLLTSDKKITNEVLKAFNFFSANYRKDKFYHLLVSPFGLR
                     NKLVSLIENETRNAKAGKAACIDIKINNLTDEEIIESLYRAGQAGVRIRLIVRGMFSM
                     TVGVDNVSENIRAIGIVDRYLEHSRFMVFCNGGEELVYITSADIMPRNLDRRIEVTCP
                     VWDPSLRKEITDIFNIQWNDNVKARILDAELSNMMVPAGETALRSQTEIHRYYSEISS
                     PA"
     gene            10858..11220
                     /locus_tag="PKK03_07880"
     CDS             10858..11220
                     /locus_tag="PKK03_07880"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNX84359.1"
                     /translation="MKNEDHFGRSYFTNCGKIVYCEADGSYSDIIFQNGEKHKISKNL
                     IQISMSLPSDMFFRCHRSFLVNTYRITGFDILNKTILLDGKYVVPISSSRLKKIDCKI
                     SEFSKSNIINMIRQENSI"
     gene            11302..11571
                     /locus_tag="PKK03_07885"
     CDS             11302..11571
                     /locus_tag="PKK03_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84360.1"
                     /translation="MKTLKKLSAVLALGLILALLPVSSTAKSTNEGVVITCSANDYGK
                     CYEFNFNWCNPYMLVAAECNWTGMQANYCSLLLVKAYNICMDYIM"
     gene            11707..12864
                     /locus_tag="PKK03_07890"
     CDS             11707..12864
                     /locus_tag="PKK03_07890"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HNX84361.1"
                     /translation="MKSHQYLHVIIPVFVLVTSFSACKKQHRVSGTRSYSDSTSIAVN
                     LNETSKISVFDIFSKISVIPLETDSNSVIEQISRVIPHNNRYYILDRKQSAVLIFDSN
                     GNFEKKIQKLGVGPGEYSLLYDFNINPFTNELELLNPRGELLSYTLDGEFISSLDIPM
                     RNASKFLPITEDMIIFYTSYEEEKLHYYSRSQDKILKKEFHFPETILSTPLIANNSSP
                     FIKHGDQTTFFLGFSNEIYDIDTSGIRLRFRWDFGEHNFDYRDLPEDKDPLYYVALLK
                     SGNMAHSFYSYQENQHIIMTRFAFDKQWVTLIYNKTNGESTIIRRFTEDIMPPSFPVL
                     SEEEILGFENSMMAHLFVNEFVVDEPSRSVLANLKPEDNPILIRYFFKNQK"
     gene            12873..13445
                     /locus_tag="PKK03_07895"
     CDS             12873..13445
                     /locus_tag="PKK03_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84362.1"
                     /translation="MIVIAALSCIGAVSSTLLLVKNGTGQENSEQSIENFYMNELIKA
                     SIENECIELGNPIVYPGKRIEEQVPLFDIVDQECLVFRFSGAACDVCVDFVLEKIKNR
                     FSDFSSNPRILLIGSNLNPRVKEDYYGKSVLSYASDDLGIPFEEYQIPFLFILDRDRT
                     CKMIFIPEKALPELTDLYLDTVLKRYFTAE"
     gene            complement(13504..15102)
                     /locus_tag="PKK03_07900"
     CDS             complement(13504..15102)
                     /locus_tag="PKK03_07900"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNX84363.1"
                     /translation="MKNDSLPKIFLLFLITAFSCNNGEKKGTDSADSFRFEEYTITQL
                     QQGYDNSEFTVTDVVQAYLDRIAQIDDEGPVLNAVIQVNPDALTIAAQLDAELRDGKK
                     RGPMHGVPVLLKDNIDTHDQMETTAGSRALTGSHPLQDSYIAIKLREAGAVIIGKANL
                     SEWANFRGNLSSSGWSGVGGQTKNPYVLDRNPCGSSSGSGVAVSANLTMIAIGTETNG
                     SIVCPATANGIVGIKPTVGLLSRSGIVPISFTQDTPGPMGKTVSDAAAALGAMTGIDP
                     NDSKTAASEGKYLTDYTTFLKEGGLKGKRLGWYTAARGAHFKVDTLMQHAVDFLKSQG
                     AEVIEIDNIYSREVGSLSFQVMLYEFRDGLNKYFASLGENAPVKSVEQLIEFNRNDSI
                     ELRFYDQELLIEAQKKGDLTSQEYLEALEKMNRLVRDEGIDKVMKEHNLDAFIAPTGS
                     PAWKTDLVNGDSYTVSSSSPAAVAGYPNITVPMGYVDGLPVGISFFGRAWSEPLLIEI
                     AYSYEQGTKFRKAPQFLPTLPYED"
BASE COUNT         3962 a         3694 c         3821 g         3721 t
ORIGIN      
        1 ggtctgaagg tctgagggtc tgaaggtctg agggtctgaa ggtccggagg tccggaggtc
       61 cggaggtccg gaggtctcaa atctgagagc ctgagagtta aaagattcaa agacatcaga
      121 ccttcagaca gagcgaagcg acagaccttc agactactac ctgtaaaggt agacggttcc
      181 ccggaactcc cggcccttgt attcaacatc atcatacccc tcggcgcgga ggacatagta
      241 gtatgcgccg ggctcagccc tcctttcaga gtagttgatc cggccatccc atcccaacca
      301 gttctggaga tcctcaccgt cgccctggta gtagtagacc cggtgaccgc tcttggcaaa
      361 tatttgcaga ttcaggaaac ggattgattt cttgtcgggg acaaaataat cattgaggcc
      421 gtcgtcattt ggtgtgaaca cattggggat ctgcagtgca gaagggagta cctctatctt
      481 cgccgtggca gagtcacggc atgtgagctc gctctcaatg acgagtgaaa cagtatattc
      541 tcccggtata taataagtat gtgtgcccgg gtcctgcatc gttgataccg aatcatcacc
      601 gaacttccag agatactttg atgccctgac agatttatca gtgaatgtga tctccagcgg
      661 tgactcgccc tcaaccggat cggctgtgaa ctcagccttg acatggatcg aggtataatc
      721 aaatgaagcc gaagttgagc atccgaagct gtcagtgacc tgaagcgtat atctcacatc
      781 ggtcagcggc ggagagaatg ttatgggatc aatctcaata tcgggatatg gtattgccga
      841 tgccggcgaa gatgaccaga ggtgcttcac accatttggc agcagccggg aaacgttggt
      901 caacggatca tagtatgtaa agggatcagc cgcttttgtc ccgtcgaggg ctacataatc
      961 gcatgtgtag tgctgcaggg cagcgcctgc cacaggcatg ctgaggttca cccatgcata
     1021 gaactcattg ctgtatcctc cgtcgccggt gatctgtacc ctgtatcctc cttcagcaag
     1081 acctgtggct gttgatgtta tacctgattc agtcttgact ggtatcgtgt aaccgcctcc
     1141 ggactggttg tacctggtcc atgtaaaggt gaagggtgcg gttccacccg ggcttgaggc
     1201 tgtcagtgtt ccggtatctc ctggtccggt ggcgcaaaag atataaacag aatctgtccg
     1261 gggagtggcc ggatagttag ttggcctgac agtgcgactg cccggggcag tgatctgcgc
     1321 ctgcactgcc atcggcaaca ggatgattat gagggccagg taccttgaca cacctagtta
     1381 acggatttag tcattaatat gatatgcaaa cctaataaaa aatgccgttc tttgggggat
     1441 taaaattctg cattctcatt catgtttcgc gactatctga aagagcttaa cgaggcacag
     1501 caggcggccg ttgtcaacac gtccggaccg gccatggtta ttgccggagc ggggtcagga
     1561 aaaacgaggg tattgactta ccggattgcc tatttactcc agcagggtgt tcaggcgcac
     1621 cgtatactgg ccctgacctt cacgaacaag gcagccggtg agatgaagga gcggattaac
     1681 aggatcgtcg gccccgaaaa ggcgcgatac ctgtggatgg gaaccttcca ttcgatcttc
     1741 gcgaaattcc tccgttatga cggccatcac ctgggataca aaagcaactt caccatttac
     1801 gaccaggata acacccgaag cctgatacgg acgctaataa aggagatgaa tctcgatgac
     1861 tcggtttaca agccggcatc cgtcgctggt cgcatttcac atgccaagaa caaccttata
     1921 accgcccagc aatacagttc ggttccccag atcaccgagt cagacaaggc ttcacgtgta
     1981 ccggaaatgg ctgcaatata caggcattat gcagccaggt gcttcaaggc gaatgccatg
     2041 gacttcgacg acctgctgat gaacataaac atactgttcc gcgactttcc cgcagtgctg
     2101 gaggagtaca ggaaccggtt cgactatgtt ctggttgacg aataccagga taccaactat
     2161 gcccagtaca tcatcgtcca caagctgtcg gaaaatcaca ggaacctgtg tgtggtgggt
     2221 gacgatgcgc agagcatcta ctcattccgc ggagcccgga tagagaatat cctcaatttc
     2281 aaaaacgatt accccgacta ccagttattt aaactggagc agaactaccg gtcgacccag
     2341 acgatagtca atgcagccaa cagcgtgata gagaagaacg ccggccagat ccggaagacc
     2401 atattctcga agaacgagga gggcgaaaag atcagggtca ttcagacaac catagacagc
     2461 gaggagggca tcctgactgc ttccgacatt cttgacacaa ggatgcgcga gaggctcgac
     2521 tggaaggatt ttgcaatact ataccgcacc aatgcccagt cgcgtatctt cgaagagatg
     2581 ctgcggaagc gcaacatccc ctataagata tacggcgggc agtcatttta tgagaggaag
     2641 gagatacgtg accttatagc ctacctgagg cttgttgtca atccggacga cgaagaagca
     2701 ctcaggcgca tcatcaacta cccgagaaga ggcattggtg acactaccgt catgaagctg
     2761 atggatctgg cccgttcact tgatctgagc atatggtcaa taatcacttc acaggagctg
     2821 agcgcgaagt accttgctcc cgccctgcgg acgaagataa gttacttcac gggtctgatg
     2881 atgcctgtga tggctgtggc ggaaacggcc accgcatatg acattgcatt taatatggct
     2941 tccgcaggcg ggattctcaa cgagctgaaa cagggacagg tgcccgagga gattgacagg
     3001 ctcgaaaacg tgcaggaact gctgaacggc atcaaggaat ttaccgaatc agcagtcacc
     3061 aacggagagc ctgcggatat tgcgacctgg ctgcagtctg tctccctgct caccgaccag
     3121 gacatggaga agccggaaga cagggacaag gtcaccctga tgaccattca ttctgccaag
     3181 gggctggaat tcaagtatgt atatatcgtt ggcctcgaag agaatctgtt tccatcagca
     3241 atgaatctct tcaatccgcg tgagttggag gaggaacgtc gcctctttta tgtggcaatc
     3301 acccgcgcat gccgtagggt gacactgagt tatgccctca acaggtacaa gtggggaacc
     3361 cttgagagga gctctccaag ccgttttata ggtgagattg agagtgagtt tctcagttac
     3421 cctcagacgg gaggaaagcc tttcggccag agggcggttt acgaaccggg acctgcaggg
     3481 ataatccccg agcagtcccg cccgttctcg cctccggcga ggatgaccag gctgcgtgat
     3541 gcgaagacgc ctccgacggc ggacggcgtg aacggtgcag gaccggcagg cagcggagca
     3601 accggtcaca gcgccggcag aagcacaggg atgggtggcg gcgtcatcgg caacggcata
     3661 aacatggggc tggagcccgg agacagggtt tctcatgaac ggttcggcga aggcgaggtt
     3721 gtatccgtcg agggcgagtc gcccaacacc actgcactca tcaattttaa aacagccggc
     3781 acgaagaaac tgcttcttcg ctttgcaaaa ctgacaaagc tttagaacgg attactgccg
     3841 ccccgatccg ccagcaggcg gatgcggggt tgagccacct cctgcgtcgg agtctcaaac
     3901 tgccgactgg tactgcctac taaaacaaag gagaccgtca tggctgaccg gccccctcag
     3961 tgaagattat gaagttcaaa taaatggatg ttcagtaaaa ttatgcctca tttgcggcgg
     4021 catctccctg caccggttct gctgccggca ctggctcaac ctgccacata cccttttcaa
     4081 tcagcaccga gtgatagaga gttgcaaatg agctcttgac ccacatgtcg gatacaagca
     4141 ctcctataaa gaagcagatg aatccggcga tgtatatgaa gaaggcaatg aaacccatga
     4201 ggaaaattgc ccacccgtga ccgcgcgtca gcctccagct ctcctcagcg gccctgatcg
     4261 ggtcaagacc cttgtccatg acgaggtagg gtgtgaatgt caggcggcac gcaaggatga
     4321 tacccggtac gatcagaaac acaaggccaa tgccgataag cgctgtaagc agaaggttgg
     4381 tcagtactat attaaggtag ttcttccgga aaccgctgat gagcaggtca aaatccggag
     4441 ttatctgacg ggatgcctgc acgaacatca tctttgcccc gtacctgaat accggcagca
     4501 acagaaaaat atagagcaga actatgagtc ccagaaaagc tgccgccagc ccgaaggcgc
     4561 cgaatttgaa gaaatcatgc atatcaaacg ggatgtttcc gaagtcaccg ggattgaatt
     4621 cccacttgga atgtgatccc agcatcgggg aaccgatgat tgccaggacc aatacaacca
     4681 gcaataacct cagaaaatta tctgacatag ctctccatcc tgtgccgtaa gatccgctga
     4741 aggagggaac atacctgtaa ataccttttg cttccatatt agtttgcttt ttactgtttt
     4801 gaaaaatttc gtttgtcgaa gcaaatctat tacggaaccc tcgggcggaa ttcatacctt
     4861 agtagtgatt ttgaaaaaca atacttaagt cttgccgtca atacttaagt agtgtctgta
     4921 tatcaattga ttattcgaaa acgggaatgg agtttatcag tcttgtcatt tttattttct
     4981 ccgcggcgat ggctgcggga agcattcttc tcgttggcag gatgcttgag gagtaccccc
     5041 tcccctttct ccggtcactc ttttaccata atcttttcat ctcggcgttc ggtttttacg
     5101 gtatctgggg acagttcata atttttgccc tggcggggga caggcttgat ccggcactct
     5161 attcaaccgt atctgttatt tcgcttctcc tcggcctccc gttccttgtc ttcgggtgga
     5221 tgatgctcat caggttttcc gccgaggcat ccggtgcacg cctgccgggg tacttcaacc
     5281 tcgttttcct ggtggtaaat ttcggggcga ttgctgcact tggcctgctg atccgggata
     5341 tggaattcac tgatgccctg cccctattcc ggtattacta tgtggctgcc tcgctgctct
     5401 ttgcgattat ctccgcactt atccttttga aggggatctc tgtttcgccc ggcatggcgg
     5461 accgtcttca cctggcactg ataatcgccg gaggcactct cttacagtcg ctggtgctca
     5521 tattcgtgcc ccattcagta tggatggccc tctccttcgt gtttctcctg tttgcagcta
     5581 taacatctgt atcgctgtac ctcagttaca gggccgatct gaagcctgcc attatccctg
     5641 accagctgcc gttgccgcgc ggtattgagg atttcataag gcgaaacgag atctcaccgc
     5701 gcgaggccga gatcatcacc gaaatatgca acggcctgag taaccaggag atagccgaca
     5761 agcttttcat cagcctgcag acagtgaagg accacacctc gcgcatatac atgaagacca
     5821 acgtgaggaa caggatgcag ctgatggcac tggtaagagg aatggatacc ggcagccatc
     5881 ccggtaaaaa tatctaaaag gagattccca gtatatcctg cagtttcttt cccggtgcaa
     5941 aaatccggta agcatacggc cataccttct ccaggtacct gtcctgcctg tctctgatga
     6001 ctatcgccag ttcattgtcc atatcactgt tttccggatc cccgtaacgg tagcccgtaa
     6061 gtaacattat ctgtgccagg tcattgtatt tcccgaggtt ctgtgtcatg accccgagtc
     6121 tcttctcaag ctgcctgaca gtctgcggcg ccaggtgcct gaagaagatc agctggtaca
     6181 tgaacgtctt cgattttttt ctgaactcat gcaaaagccg ggctgcaggc ttgttgcggc
     6241 aggccaggta agctgccgaa gcagtggcat ggctcctccc gagttcaccc agcatcagcc
     6301 tcatgtccgg ctcattcagc cccaggaacc gaagcctgta ttgtgtcttt ttaagcctgg
     6361 aggcgacttc gtgtaccgat ttcgcacgat caccggcctg ctcccatgtt gaatagggtt
     6421 tgcggagcag ttcctgaatc ttctcgttcc cgccaagcct tatgaaaagc tccggattat
     6481 ccttcttcag tgcctttacc gtcttgcgaa gcacggcagt ctccctccac agtgccagca
     6541 ggcgtccggt ttcccttccg gcaaggtact ccctgcggta gacagcctca tccagcatcg
     6601 gcttaacaag ttttactgcg gcacggtgct tcttcatcag caccctgaca tcgtggattg
     6661 ccgtgtcatc gggcaccggc tgcatcgcca gaagatccat cgaacaggca atgtatccgg
     6721 ccatcgaaca ttttatatcc ttcgcctctg cggaatcttt aggggccatg atcagtaaat
     6781 tgacagatgc taaagttaca taaatgttac tggttttcca ccttttatct gcttcagcgg
     6841 tttacctttg accctgaccg gcatgaaaat gaccggagaa aggactttgc ttttttagta
     6901 catttgataa ggtaaaactt taacatcaat gggcaaatca aacagcaggg acagccagcc
     6961 ccggaccata ggcatcctca ccgcgggagg cgactgtccg ggcatcaacg cagccatcag
     7021 gggcgttggc aagacagcca tcgtcaagta cggcatgaag gtcatcggca tcagtgacgg
     7081 ctatgccggg ctgataaaca aggagtaccg cgagctgacg gagagtgatc tctcaggaat
     7141 cctgaccctc gggggaacca tcctgggcac ttcccgcgag aaacccttca agggaaccgg
     7201 caaaaagaag gacgagaccc gaaagccgct attgatcaag gaacactatg aacagatggg
     7261 gctcgactgc ctggtgtgca tcggcggcaa cgggactatg aagacggcca acatgctgtc
     7321 agaagaaggc ctgaacgttg tcggcattcc caaaaccatt gacaatgaca tctgggggac
     7381 tgacatgacg ttcggattcg actctgcggt gaacatcgcc actgaagcca ttgacaggct
     7441 gcactccacg gccaactcgc acaagcgtat catggtgatt gagatcatgg ggcacaacgc
     7501 cggatggctg gcactttatt caggtatggc tggcggggga gacgttatac ttatacctga
     7561 gatcccccat aatgaaaagg ttgtgatgga ttaccttcgc aaacgggccg acaacaacaa
     7621 accatactcc attgtggttg ttgcagaagg gattgcaata cctgagggag agatctctcc
     7681 ggcacgctac cttgcgagaa gaatcatgga agagaccggg ctggaaacaa gagagacagt
     7741 tcttggttat atacagagag gcggatcccc ctcgccaatg gaccgggtgc tggcatcgcg
     7801 cttcggcgca gccgctgcca accttattgc aaagggcgaa ttcgggaaaa tggtggcaca
     7861 gcgcaacggc gaaatcagct cagtgaaact ctccgaggtg ggtggaaaaa caaagcttgt
     7921 gggcaaggac gacccgatgg tcagacaggc aagggagatg ggcacatgct tcggcgatta
     7981 acgcatagac agtccgggga tatgggtacc tgcttcggcg attgacacat aaacgggaca
     8041 gggtgacctg gaaccggaaa ttatattcat aaaagactat ggttaagaag agcaaaacag
     8101 aagagagcgg aacaagaaaa ctgattctca taagacacag caaggcagag gacttctcct
     8161 ccgaaatctc cgattttgag agatcgctga ctgtcagggg caagataaac agccggctga
     8221 tggcagagat actcaaggca aagggggaag agcccggcag ggtgatcagc agccctgcct
     8281 tccgcgcact ggagactgcg ctgatcttct gccgcgaata tgatataagc cctgcagaga
     8341 tcaggctcgc ccctgaattg taccatggcc tgagggaatc ggagttcctt ccttttatcc
     8401 agcagcagag cgataatgac cacactgtta ccctcttcgg tcataacccg cttattactg
     8461 aactggctgc gtactttgcg tctgacgagc ctgagatatt gcccaaaacc ggtatctact
     8521 gcattgaatt tcaggccgca ggatggcagg atgtggaacc ggaaagcggc agaaccctct
     8581 atttcctcac acccagggag ctggcatgag caaagcatac attcccaagg agataagctg
     8641 gctctcgttc aacggacggg tgctccagga ggccgcaaac agcgaggtgc ccctgctgga
     8701 acggttcaat ttcctgggta tctattcaaa taatctcgac gaatacttca gggtacgggt
     8761 ggctaccctg aggagactca ccctttacgg cagcaaggcg aaagggatac tgggctacag
     8821 cccgaaggcc acacttaaga aaatccagga gatcgtcctc agccagaacg atgtttttga
     8881 gaagacctat accggtctcc tggcggagct gaagaaacac cggatcaatt tcgtgaacga
     8941 gcagcagctt gacccggaac agacagaatt cgtccgctct ttcttccgca aggaggtcag
     9001 atcgaggctt atgcctttga tcataagcaa ggaaaacaag ctgcccgacc ttcctgatga
     9061 tgcagttttt ttcgccgtac agctccgtaa taccgcaaag gacaggaaga tatatgctct
     9121 gcttgaaatc cccccgaagg agatccttcc caggttcatt atgctgcccg gcagaaaggg
     9181 cagcaggtat gtgatcttcc ttgacgacgt tataaggtgg ggtctgcatg agatattcag
     9241 caccctgccc ttcgatgagc tctccgccta cactttcaag gtgacaaagg atgctgaact
     9301 tgagattgcc gacgatatct ccgaaagcta catcgaaaaa atctcgaaga gccttcagtt
     9361 gcgaaggaag ggcaatcccg tacgttttgt ccatgacagg gagatgccgc aggaattgct
     9421 gaaaatcctc tcaaagaagc tgaacctggg aacggatgat gtgatcatgt cgggaaacag
     9481 gtatcataac ttcaaggatt tcatgaggtt catcgagatt gaagggtcag ggctctccta
     9541 cccgaaactg ccgccggtga gacatcctgc cctgcactac ggcaagagca tcctctctgc
     9601 tgtgcggaag cgggatattc tgttctattt cccttatcat ccgtttgaac acttcatcga
     9661 tctgctcagg gaagcatcaa ttgatcccta cgtcacgtca gtccatatca ccctctacag
     9721 gctggcgcgc aactccagtg taatcaacgc actgatgaat gccgcccgaa acgggaagag
     9781 cgttacgacg gtggtggaac tgcaggcccg gtttgacgag gaggccaata tcctctgggg
     9841 gaacaagtta caggacgaag gggtgaaagt catatatggg gtgccggggc ttaaggtgca
     9901 ctccaagctg tgcctcatca caagggagaa ggaaggggtg gtgcagaggt acgccgttgt
     9961 tggcaccggc aacttcaacg aggagactgc cagggtgtat accgatcacc tgttgctcac
    10021 ctctgacaaa aagatcacca acgaggtgct caaggcattc aactttttca gtgccaacta
    10081 caggaaggac aagttctacc acctgcttgt ttcacctttc gggcttcgca acaagctggt
    10141 aagcctcatt gagaacgaaa ccaggaatgc caaagccggg aaagctgcat gtattgatat
    10201 taaaatcaat aacctgaccg acgaagagat aattgagtca ctttacaggg ccggtcaggc
    10261 cggagtgcgt ataaggctga tagtcagagg catgttttcc atgacagtgg gagttgacaa
    10321 tgtaagtgaa aacatcaggg cgatagggat tgtggacagg tatcttgagc attccaggtt
    10381 catggttttc tgcaacggcg gcgaagagct ggtttacatc acctcggctg acatcatgcc
    10441 gcgcaacctt gaccgcagga tcgaggtcac ctgcccggta tgggatcctt cattgaggaa
    10501 ggagatcacg gatatcttca acattcagtg gaatgacaat gtcaaggcgc gcatccttga
    10561 tgcggaactg tctaatatga tggttcctgc cggcgagact gccctcaggt cgcagacaga
    10621 gatacacagg tactactcgg agatcagttc accggcgtag gaggccagag tagtctggat
    10681 tggcataggc tgaaaatgcg agggatcgaa aataaccctt cctcaattgt taaatatcct
    10741 gcgatattta gcacttaatc tcaaaatact ttcataaatt tattgccgga atcctttgat
    10801 gtgctgattt tcagggattc tttaattcgc tgcaataact gtatttattt agtaactatg
    10861 aaaaatgaag atcacttcgg tagaagttat tttaccaatt gtggtaagat agtgtattgt
    10921 gaagccgacg gaagctattc tgatatcatc ttccagaacg gagaaaaaca taaaatatcg
    10981 aagaacctga tacagatttc aatgtccctt ccttcggata tgtttttcag atgtcaccgc
    11041 tcattcctgg taaatactta tagaattaca ggcttcgata ttcttaacaa aaccattctt
    11101 ctggatggga agtacgtggt tccgatatca agcagcaggc taaagaaaat tgattgcaaa
    11161 atcagcgagt tttccaaaag taatataatc aatatgatcc gccaggagaa tagcatttga
    11221 ctttgatttt tgtaaaaaag atttgcattt gacactgatt gttaggaatt ttgaaatatg
    11281 tttaactaaa cctcgtgcaa aatgaaaaca cttaagaaac tttcagccgt attggcttta
    11341 ggattaatcc tcgctcttct gccggtttca tcaactgcaa aaagcacaaa tgaaggagta
    11401 gtaattactt gttcagcaaa tgattatgga aaatgctatg agtttaattt taactggtgt
    11461 aatccctata tgctcgttgc tgcagaatgt aactggactg gaatgcaagc caactattgc
    11521 agcttgcttc tggtaaaagc ctataatata tgcatggact atattatgta aattctcaat
    11581 atgagggtga tattgactta ctctgcgata gtctccacgg gtgattaacg cggtagaatg
    11641 tagctgttgc tcaatccgga acaatcaaat gtaattgtat cattgaatgt tactaattgt
    11701 caaattttga aatcacatca atatttacat gttattattc ctgtgtttgt acttgtgact
    11761 agcttttctg cgtgcaagaa gcaacacaga gtctctggaa ccagatcata ttctgactcg
    11821 acatccattg ctgtcaattt aaacgaaacc agcaaaatat ccgtatttga tattttcagc
    11881 aagatatcgg ttattcccct ggaaaccgac agtaattcgg tgatagagca aatatcaagg
    11941 gtaatcccac ataataaccg ttactacata ttggatcgta agcagagtgc cgtgctgata
    12001 tttgacagca atggaaactt cgaaaaaaaa attcagaagt taggagtcgg gcctggagag
    12061 tattcattgt tatatgattt caatatcaat cctttcacca atgaactgga gctgctgaat
    12121 cccaggggtg aattactgtc atatacactg gatggggaat ttatcagctc cttagacatc
    12181 cctatgcgta atgccagcaa gttccttcct ataacagaag atatgatcat tttttatact
    12241 tcttacgaag aggaaaaact gcactattac tcaaggagtc aggataaaat actaaaaaaa
    12301 gaattccatt ttccggaaac aatcttaagt acgcccctga tagctaataa ttccagtcca
    12361 ttcattaagc atggagatca aaccacattt tttctgggtt tttctaacga aatatatgat
    12421 atcgacacct cagggataag gcttcgattc aggtgggatt tcggggagca taactttgat
    12481 tacagggatc tccctgagga taaggatcca ttgtactatg tagctttgtt gaagtctgga
    12541 aacatggccc acagtttcta ttcatatcag gaaaaccaac atatcatcat gacgagattt
    12601 gcattcgata agcaatgggt taccctgatc tacaataaaa caaacggaga atctactatt
    12661 ataaggaggt ttacggaaga cattatgcct ccttcatttc ctgttctatc cgaagaagag
    12721 atattgggat ttgaaaactc catgatggct catctgtttg taaacgaatt tgttgtggac
    12781 gagccttccc gatcagtgct tgccaatctg aaacctgaag acaacccgat cctaatcagg
    12841 tactttttta aaaaccaaaa atgaaaccat tattgatcgt cattgccgct ttatcttgca
    12901 taggcgcagt ctccagcact ttacttctcg tgaaaaacgg gaccgggcag gagaattcgg
    12961 aacagtcaat tgaaaacttt tatatgaatg aactgatcaa ggcttcaatt gaaaatgaat
    13021 gcattgagtt gggcaatccg attgtatatc ctggaaaaag gatagaagaa caagtgccgc
    13081 tatttgatat tgtcgaccag gagtgcctgg ttttcaggtt ttcaggagca gcctgcgatg
    13141 tgtgcgtgga tttcgtactc gagaaaatca aaaacaggtt cagcgatttc agcagcaacc
    13201 cgaggatact gcttataggc agtaatctta atcccagagt aaaggaagat tattatggta
    13261 aaagtgttct atcctacgcc tccgatgatt taggtatccc gtttgaggaa taccagatac
    13321 cctttctctt tatactggac agggacagaa catgtaagat gatctttatt cccgaaaagg
    13381 ccctgccgga attgacagat ctgtatcttg atacagtgct taaaaggtac ttcaccgccg
    13441 aatgatccgg aatttaaact ttctttttca ggtaatgctg accggtgtct tcattccatt
    13501 cagttagtcc tcatacggca atgtcgggag gaactgcggg gccttgcgga attttgtacc
    13561 ctgctcatat gagtatgcaa tttcaatcag cagcggttcg ctccatgccc tgccgaagaa
    13621 tgagatccct acaggcaggc cgtcaacgta acccatcggc acggtgatgt tggggtatcc
    13681 ggccactgca gcgggtgatg aactgctgac ggtgtaagag tcgccgttga ccaggtcggt
    13741 cttccatgcc ggcgagcctg tcggggcaat gaaggcatca aggttatgtt ccttcatcac
    13801 cttgtctatt ccctcatccc ttaccaggcg gttcatcttc tccaatgcct caaggtattc
    13861 ttgggaggtc aggtcacctt tcttctgagc ttcaatcagc agttcctggt cataaaagcg
    13921 caactcgatg ctgtcattcc tgttgaactc tatcaattgc tccacgcttt tcaccggggc
    13981 gttttctcca agcgaagcga aatacttgtt cagcccgtcc ctgaattcat agagcattac
    14041 ctggaatgag agcgagccca cctcacgact gtagatattg tcaatctcga tcacctctgc
    14101 gccctggctt ttgagaaagt caaccgcatg ctgcatcagg gtgtcaacct tgaagtgtgc
    14161 tcccctggcg gctgtgtacc acccgagtcg tttgcccttc agtcccccct ccttcaggaa
    14221 ggtagtgtag tccgtcaggt atttgccttc agaggctgca gtcttgctgt cgttcgggtc
    14281 aattcccgtc attgcaccaa gggctgcggc agcgtcgctt acggtcttgc ccatcggacc
    14341 gggagtgtcc tgcgtaaagg agatgggcac tatgccgctg cgactgagca aacccactgt
    14401 tggtttgata cccacgatgc cgttggcagt ggcggggcat actattgagc cgtttgtttc
    14461 ggtgcctatg gcaatcatgg tcaggttggc tgagacagct actcccgagc cggagcttga
    14521 gccacacgga ttgcggtcga ggacgtaggg attcttcgtc tgtccgccca cgccgctcca
    14581 tccgcttgat gagaggttac cacggaagtt ggcccactcg gagagattgg ccttgccgat
    14641 gatgacggcg ccggcttcgc gaagcttaat cgcaatatag ctgtcctgca acgggtgcga
    14701 gccggtaagt gcgcgtgatc ctgcggtagt ctccatctgg tcatgggtgt cgatgttgtc
    14761 tttaagcagc accggtacgc catgcatggg accccgtttt ttaccatccc tcagctctgc
    14821 atccagctgt gcggcaatgg taagcgcatc aggattgacc tggattacag catttagcac
    14881 aggaccttca tcatcgattt gtgcaattcg gtcaagataa gcctgtacca catcggtaac
    14941 ggtgaattca ctgttgtcat aaccctgctg aagttgtgtt atggtgtact cttcaaaacg
    15001 aaaagaatct gctgaatccg ttcctttctt ttctccattg ttacatgaga aagctgtaat
    15061 caggaataac aggaaaattt tgggtagtga atcgttcttc atggccgata ttaataatag
    15121 aaaatgtatc agctaaagta aagtaataaa taattaaaga aataatgtcg gtattatttc
    15181 tttccatagt gcaggata
//
LOCUS       DAOPXU010000066        14710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_4429_length_14710_cov_14.684681, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000066 DAOPXU010000000
VERSION     DAOPXU010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14710
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4429_length_14710_cov_14.684681"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..985)
                     /locus_tag="PKK03_07905"
     CDS             complement(266..985)
                     /locus_tag="PKK03_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNX84364.1"
                     /translation="MNTSLKRVSLAAVVILFGLGVSGQNQIPLNGPWEDEKFFNAGYT
                     IGFNVMTFGINPSEQFTLTDSVYPARGSVSPGLNIHLALNFRLNQFFDIRLLPGISFG
                     QRNVKYSSDRVHDTVFSPQKVESSFIEVPLQLRYGWRMQNIKPYVIGGINYRYDFYAQ
                     EKYRLERPVYLRLNRPDLYYEAGAGVGFYLGELRLALELKISNGIADVLAHDPHPEYP
                     EYCNTIEKLKSRIWVLSLHFE"
     gene            1123..1458
                     /locus_tag="PKK03_07910"
                     /pseudo
     CDS             1123..1458
                     /locus_tag="PKK03_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
     gene            1528..2985
                     /gene="aspA"
                     /locus_tag="PKK03_07915"
     CDS             1528..2985
                     /gene="aspA"
                     /locus_tag="PKK03_07915"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HNX84365.1"
                     /translation="MRNANTRILGYNAQYDSGSTRSESDLLGEREVPVESYYGVQTLR
                     AMENFNISGVTLNFFPSLIEALSMVKESAAEANYELGLLDRSVRDAIVLTCREIQNGK
                     YHNQFVVDMIQGGAGTSTNMNANEVIANRALEMLGHEKGDYARCHPNNHVNLSQSTND
                     AYPTSVKIALYNSNRKLVAVLKTLVESFRRKGEEFSHVLKMGRTQLQDAVPMTLGQEF
                     EAYAVTLAEEIERLNLNARLFLEINMGATAIGTGINSEPGYSDKVTRHLRRITGLDLV
                     LASNLVEATQDTGAFVMYSSAVKRLAVKLSKICNDLRLLSSGPRAGINEINLPPMQPG
                     SSIMPGKVNPVIPEVVNQIAFKVIGNDLTVTMASEAGQLELNVFEPVIVHSVFESVEM
                     LINGMNTLRFRCIDGITANEERCRSMVENSIGLVTALVPWIGYKSANELAMEALASGE
                     SVYSLVLSKNLLSKEKLDQILAPENMLRPGKMKDE"
     gene            complement(3004..3225)
                     /locus_tag="PKK03_07920"
     CDS             complement(3004..3225)
                     /locus_tag="PKK03_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84366.1"
                     /translation="MDFKTGIGSSMKKTVFRIAGVILVAYMLSFASCDTVDICWYCEN
                     PHNPNEWQQVCNAGTKTKLESYGYVCTPF"
     gene            complement(3321..6692)
                     /locus_tag="PKK03_07925"
     CDS             complement(3321..6692)
                     /locus_tag="PKK03_07925"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNX84367.1"
                     /translation="MRLRLIAISLAILICSAAAGQIEFSSGSGYRYLKGSGAASLSAD
                     WMTSQFFDSEWSEGNAPFRFGDGIGGVVLTDMMNRYSTLYLRSSFNAFSIDRIKTIRF
                     RVNYDDGFVIWVNGARVMARNAPATLSYNALAVANHESGTFETFYLDSTDVPLVEGTN
                     LLCIQAFNVSPSSSDFYFDVAVEASLSLPEVPETLPLNFDHEAGFCEAPFDLAISSGQ
                     SGYDVIYTIDGSNPQTSSTAIRSNTAVTLRIDPVISAGRPATPVFIVRASLVTEGYSP
                     SRPVTKSFIFPDKVLTQGHPGGGWPSSPVNGQVIDLGMDPDVVNDSRYSQQMKASLMD
                     IPSISLVTDLADMFGASGGIYVNAYQHGEEWERQCSLELINPDNTIGFRVNAGVRIRG
                     GASRTSDNPKHAFRLFFRKEYGPGKLLYPLFGNEGAAEFDKVDLRTEQNYSWSKDGDT
                     HNTFLRDIFSRDTQRDMGQPYTRGKYYHLYLNGMYWGLFQTEERPDADYAETYFGDGD
                     DDYDVIKVSVESWPYFNEATDGTMASWQELYNRCNRGFASNTDYFALEGKDQNGNPVK
                     NTRVWVDIDNLIDYMLVIFYTGNFDAPVSAFYGNAMANNYFAILNRKNRGEGFVFLAH
                     DSEHSMFVRPYSLSNGINENRVSISNPAMTVSGISNFQPQWLHHRLISNAEYRLRFAD
                     RAYKRFAAGGALSPAKCVNRFNARKAEVEMAIIAESARWGDAKTSQPRDKIDDWLPEV
                     NDLVNNFFPARSDIVMSQLRSASLYPSLEPTVVKESGITILEEKVVYTPLTIRLENPN
                     SRGQIYFTTDGSDPRQLGGTVSSNAVQAQNGSTVGVSGSMIFRSRILDGQTWSALKEV
                     RFSAVMEDYSNLRVTELHYHPADLVRSADTVDGKDLEFIELKNAGNGAMNISGITLDT
                     AVYYRIPDGTMLPPKGFWVVASKPEEFYSYYGMSPSGNFSGNLSNAGEYLLITDKSGN
                     RIFAFTFSDDSPWPVEADGDGYSLVSKAADPSGNPDAATYWKHSMNKGGSPFADDPVS
                     TAIERLINGERGELRLWPNPTSGVVVIDADEEISGPMKLRFISVTGATALVLDVEGAT
                     LVDLSEHGLAPGVYTVTAWHAGVSYMTRLVYIPER"
     gene            complement(6935..7156)
                     /locus_tag="PKK03_07930"
     CDS             complement(6935..7156)
                     /locus_tag="PKK03_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84368.1"
                     /translation="MDYNFHIMSLPHGRTYLMNLTRNVVIRSSPGKLSKFYAKEKGGK
                     EFELHHLSDLLQDTLKAARRITRVEYVQF"
     gene            7369..8709
                     /locus_tag="PKK03_07935"
     CDS             7369..8709
                     /locus_tag="PKK03_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX84369.1"
                     /translation="MNRERGRQFYFSVVAALIVLMTPCIKGQTGNEPGTVPGSADDSL
                     KCVDYVSWYKAAMNEGNISGALQPWRQALAGCPSVSEELYTDGELIYRSLFDITGEQK
                     YIDSMVMILTQRTYFYNNKPSNDLHKAEILLELAGDDPAYLGLCYNILAEAAGSFPEQ
                     MECHHFVQMAALAASLYAMGVIDGEELGNAFVTAIGTVDWRMENHIAGCSSADDLENM
                     ETFYRTNGAMTCEGLETLYGEKLDRNFRDTAFVSKVFAMIQEAGCTRSDLYYNVAVKM
                     FANDRSAQNAVRLAELNAAAGNNDKAISYFTEAYNRDTDSIVRSEVLIRVAMMELGQG
                     KRPEARDRAEHAWQLNRKNAAALMLLAECYAGAELGNTFDNHTAYWVAADYLDAAVKA
                     DPSLRKEAERKIKVYMQNFPTREECFYRKILDEGVIFTVGGWVNEVTRVRFRKE"
     gene            complement(8801..9313)
                     /locus_tag="PKK03_07940"
     CDS             complement(8801..9313)
                     /locus_tag="PKK03_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84370.1"
                     /translation="MKKLIVLIVGCMLALATWGQDKFTVPAPSDLQKYNLAAWQWTGA
                     YVILIKYAKSLGQTVEEAAIAAGGIAAATWNSDMTFDDLVNSMVYTYVVLTPSGKVEI
                     LEQSPDSVAFSVTNFYKPLDAMLDDFKITRAELSEFYEVYAKQIANLIGVKYEVIETG
                     DIITVTVSKN"
     gene            complement(9392..9850)
                     /locus_tag="PKK03_07945"
     CDS             complement(9392..9850)
                     /locus_tag="PKK03_07945"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HNX84371.1"
                     /translation="MKKIPPFILALMLLAGCTTAPKVDITAEAEAVRNLENQMTVAYQ
                     NRDIEKLLSYGTSDILHLSPGVPPETGIEDYRGRIGSQLADTACLWETYSTKIGLIEV
                     SSSGDMAYVWGTDLLQNRTPDGIVDDPAKWVDIWKKINGEWKIVLNIWNR"
     gene            10460..11080
                     /locus_tag="PKK03_07950"
     CDS             10460..11080
                     /locus_tag="PKK03_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HNX84372.1"
                     /translation="MKTFRQISSGLGVAAGCNTVLRNLKLRLTAKSVAPKVILIFMTF
                     FLNTYAVPGGKPEMTEEMQLRAEKDLFYFQVRYLPFSEELLIKCLGYEGIKYQEVVLL
                     QSRLETGYYTSDIFLNGNNLFGMRYPAFRPTVATGTYKEHARYAHWSDSVIDYALWQE
                     YYMSRGYRIGEGQDTDFYLVFLKCIPYAEDKMYVSKLVTMSGGDIT"
     gene            11179..11832
                     /locus_tag="PKK03_07955"
     CDS             11179..11832
                     /locus_tag="PKK03_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84373.1"
                     /translation="MKRIVAVLLFSLILFSSFCQSQGVELSHYVFPDFTNGSVLMKSG
                     QQHQALLNYNALSEEMIFVDKGTKLAISKEEKEKIDTVYIKGRKFFVLDGRFLELIYR
                     SGYELYAEHRCDVKYPGKPAGYGGTSETSSISTYSGVYSGGILYELRLPDDFSIKPYL
                     IYWLKKDGKINKIVNLKQLNKVFGDRKDQIKEYVGSNKTDYNDIESMVGLIRYLEAN"
     gene            complement(11825..>14710)
                     /locus_tag="PKK03_07960"
     CDS             complement(11825..>14710)
                     /locus_tag="PKK03_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX84374.1"
                     /translation="LYHPDVLSKDSIRDRKRTSLTAMRILAICEKINEELQQKQKIYV
                     LVQLMDFISFSTRAPGKELEFLETVATAFNVETGEYRDMLAFILVPGSEGFRKERLLV
                     ISSDSAHDSQGIKHLYNENLRGRLLFLNIASTNTLLMRYEGRENLLLNGQIIFSGQTY
                     VFEHGSSVRGAGINPVYFNDVEEVFVGEAFKLKVSLDARDISLRFRESENGVQKLSLH
                     EESGRLVGIMGGSGVGKSTLLNLLSGIARPDSGNVFINGHDLNTPDGKEHLRGVIGFV
                     PQDDLLFEELTVFQNLYYNARMCLNNLGRERICEEVNRILADMDLYSVRDLKVGSPVN
                     KVISGGQRKRLNIALELIREPTILFVDEPTSGLSSVDAETVMNMLKEQAYRGRLVIAT
                     IHQPGSDIYKMFDRIMILDKGGYLVFHGNPTETVVWFRKRASLANPFEDQCIACGHVD
                     TDQLLRIIETRVVNEFGKPTPVRRVSPAEWAENFTLEKGNKENDRVIAREPLPGSNYS
                     IPGPAKQALLFFKRDLLSKVADRQYMLIALLGAPLLAVLLAFFTRNVSGQAYRLSDNE
                     NLPAYMFMCVITAFFMGLILSAEEIVRDRRILKRESFLNLSWFSYINSKIIMMFLFAA
                     IQTGTFVLAGNLILGIKGMTLTYWIILFSASALASLTGLNISSAFNSVVTIYILIPFI
                     IIPQLLFSGVLVKFDSLHRSEDKQNEYVPVLGELMPARWAYEALVVEQYRSNKYENNF
                     FDFDTEISQNNWYSAFLAVALRRDIRECILFGDSPEYSGQVSDNLNRLGKYFHRLSEL
                     AGFDPPPAELISSLSPEKFNPAVAEYAEHYLDSLAGHFKAVRIANVIKKDSVTAALVA
                     SMGKQAFIELKENNTNRRLREIVLDEFGEHKIVDARGRYIQKYEPAYMKPVSDYGRAH
                     FYAPYKKTGNAEIGTLWFNLIVLWLASLLLYAALYLRLLKKAVNPVS"
BASE COUNT         3700 a         3622 c         3658 g         3730 t
ORIGIN      
        1 tcctgaccca atatcgccgt aaacttttcg tgctgcgacg caaagaggtt tttgtagttg
       61 tatttcatcg atattgtcag ttattcagca ggtatctcgt agcctgcagt tttcccatcg
      121 cctgcccaaa tttcgcgaat aaagctcata gtttgagcgc tacagccctc aaatagagga
      181 taattttgac caacaatggg actaagagga ttaatggaca gagttcttag gttacacgct
      241 tcaggccctc ttatgcctgt cacccttact caaaatgcag cgacaacacc cagattcgtg
      301 acttcagttt ctcaattgta ttgcagtatt cagggtactc aggatgaggg tcatgagcaa
      361 gtacatctgc aattccgttt gatatcttca gctccagggc gagcctcagt tcacccaggt
      421 aaaaaccgac tcccgcgcct gcctcatagt acaggtcggg tctgttcagc ctcaggtaaa
      481 caggacgctc caggcggtat ttttcctggg cgtagaaatc atacctgtaa ttgatgccac
      541 cgataacata aggttttata ttctgcatcc tccagccgta cctcagctgc aggggtacct
      601 caatgaaact tgattcaacc ttctgcggac tgaagaccgt gtcatgcacc cggtcagatg
      661 aatatttcac attcctctgt ccgaaagaaa tgccgggcag gagccttata tcaaaaaact
      721 ggttcagcct gaaattgagc gccaggtgta tgttcagccc gggagagact gatcccctgg
      781 cgggatacac tgaatcggtg agggtaaact gctccgaggg attaatgccg aaagtcatca
      841 cattaaatcc gatggtgtat cctgcattga agaatttttc atcctcccat ggaccgttaa
      901 gcgggatctg gttctgccct gacacgccaa gcccgaacag tatcacaacc gccgcaagac
      961 taactctttt cagactggta ttcataactg aatatttcaa caggtatggg gtgccaagat
     1021 agtaaaaccg atcgacttcg gccatgctga ggtttggcat taataataat cttaaattta
     1081 ttaacctttg aggcctgaca ctgattaacg gatagctgaa ccatggataa gaacgaaatt
     1141 tcacaatttc tgaagggtat tgagctcttc aggaacctta cggatgagga actgaacgag
     1201 ctcgccggac acatccgcga gaagaaatgc aactcaggat atatcctctt cagggaaaac
     1261 agtccgcgtg aagatatcta tgtaatatac gaaggagagg tagagctctt caagaggaat
     1321 gcctatggtg cagacacgag gctggcgctc ttcggcaggg gtgatttcat aggggagggc
     1381 atctgggagg agaattcgct tcattccacc tcggcgaagg ctgtaaagtc aaccactatc
     1441 ctggatatta gcagcggctt ctttcagcaa agtcccgcaa ccacccttaa gattcttcag
     1501 aatatcacaa gagtaatttc caggcgtatg cggaatgcaa atacccgtat tcttggatac
     1561 aatgcacagt atgattcagg aagcacgcga tctgaaagtg acctcctggg tgagcgtgag
     1621 gtgcccgttg agtcttatta cggcgtgcag acgctcaggg ctatggaaaa cttcaatatc
     1681 tcaggggtga cgctgaactt cttcccgtcg ctcattgagg cactctcaat ggtcaaggaa
     1741 tcagcggctg aggccaacta tgagttgggt ctgcttgacc ggagcgtcag ggatgcaatt
     1801 gttctgacat gccgcgaaat ccagaacggc aagtatcata accagtttgt ggttgatatg
     1861 atccagggcg gggcaggtac gtcaacaaat atgaatgcca acgaggtgat cgcaaacagg
     1921 gcgcttgaga tgctgggaca tgagaagggc gactatgccc ggtgccaccc gaacaatcat
     1981 gtgaatcttt cacagtctac aaatgatgcc tatcccactt cggtgaagat tgcgctctat
     2041 aactcaaacc ggaagctcgt ggcggtgctg aaaacgctcg ttgagtcatt tcggaggaaa
     2101 ggagaggagt tttcacatgt tctcaagatg gggcgcaccc agttgcagga cgccgttccc
     2161 atgaccctcg gtcaggagtt cgaggcatat gccgtcaccc tggcggagga gattgagaga
     2221 ctgaacctga atgcccgtct ttttcttgag attaacatgg gtgccacggc gataggcacg
     2281 ggcatcaatt cggagccggg ctattcagac aaggtgacca ggcatctcag gcggatcaca
     2341 ggtcttgatc tggtgctggc atccaatctt gtggaggcaa cacaggatac aggagcattc
     2401 gtcatgtatt catcggccgt gaagcgcctg gccgtcaagc tgtcgaagat ctgcaatgac
     2461 ctccgtttgc tctcctcggg acccagggcc ggcatcaatg agattaacct tcccccgatg
     2521 cagcccgggt catcaatcat gccggggaag gtcaacccgg taatacccga agtggttaac
     2581 cagattgcat tcaaggtgat cgggaatgac ctgactgtca ccatggcttc cgaagccgga
     2641 cagcttgaac tcaatgtctt tgaacctgtg atcgtacata gtgtctttga atcggttgag
     2701 atgctgatca acggcatgaa tacgctgaga ttcagatgca ttgatggtat cacagccaat
     2761 gaggaacgct gccggtcaat ggttgagaac agcatcgggc tggtgacggc actggtaccc
     2821 tggataggat acaagtcggc caatgaactg gcaatggaag cccttgcatc gggcgaaagc
     2881 gtatactctc tggtgctcag caagaacctg ctgtcaaagg agaagcttga tcagattcta
     2941 gcccctgaaa acatgttaag gccgggaaag atgaaggatg agtgaccggg cacagccgga
     3001 acatcagaac ggagtgcaaa cgtaaccgta tgactcaagc ttggtctttg ttccggcgtt
     3061 gcacacctgt tgccactcat tcgggttatg cggattttcg caataccagc agatatccac
     3121 agtatcgcat gaagcgaagc tcagcatgta tgcaaccagt atgactcccg ctatgcggaa
     3181 gactgttttc ttcatggatg atcctatgcc ggttttaaaa tccatagtgt gcaaatttaa
     3241 tcaagtacag ggctgatttc aaaatcattt tttccgattc cggtgggaaa gatacggctg
     3301 atcccggtca ggggagaacg ctacctctca ggaatgtata caagtcttgt catgtatgaa
     3361 accccggcgt gccatgctgt gacggtgtat actcccggcg cgagtccatg ctctgacagg
     3421 tcaaccagtg ttgcgccttc aacatccagt acaagagccg ttgctccggt aactgatatg
     3481 aaccgtagtt tcatcggacc gctgatctcc tcgtccgcat ctatcaccac cacgccgctg
     3541 gtcgggttgg gccatagccg gagctcaccc ctctcgccgt ttataagtct ttcaattgcg
     3601 gtggagacgg ggtcatcggc aaaaggtgac cctcccttat tcatcgagtg cttccagtat
     3661 gtggcggcat ccggattacc tgacgggtct gcagcctttg agaccagtga atacccgtcg
     3721 ccgtctgcct caacaggcca gggggagtca tccgagaagg tgaacgcaaa aatcctgttg
     3781 ccgcttttat cggttatcag caggtattcc ccggcgttgg aaagatttcc gctgaagttg
     3841 ccgctggggc tcatcccgta atatgaataa aactcctcgg gcttggaggc aaccacccag
     3901 aatcctttcg ggggcagcat ggtcccgtca ggaatcctgt aatatactgc agtgtcaagt
     3961 gtaatgccgg agatgttcat cgcaccattg cctgcattct tcaactcaat gaattcaagg
     4021 tccttgccgt caacggtatc tgcactcctt accagatcag cggggtggta atgcaactcg
     4081 gtcaccctga gattactgta atcctccatg acggcactga atctcacctc cttaagggca
     4141 ctccaggtct gtccgtcaag tatgcgcgac ctgaaaatca ttgaacctga aactccaact
     4201 gttgagccgt tctgcgcctg cactgcattt gatgaaaccg ttccgcccaa ctggcgggga
     4261 tcactgccgt cagtggtgaa gtaaatctgt cccctgctgt tgggattctc aagcctgatt
     4321 gtcaaaggag tgtagacaac cttctcctcc aggattgtaa ttcctgactc ctttacaacc
     4381 gtaggttcca gtgacgggta aaggctggct gatctgagct ggctcataac aatgtctgat
     4441 ctggcaggga agaaattgtt cacaaggtca tttacctccg ggagccagtc gtcaatctta
     4501 tccctgggct gtgaggtttt tgcatcgccc caccgggcgg attcagctat gatggccatc
     4561 tccacctccg ctttcctggc attgaaccgg ttgacgcatt ttgccggcga gagggctcct
     4621 cccgcagcaa aacgcttata ggcccggtct gcaaagcgaa gcctgtattc tgcattggat
     4681 atcagtctgt ggtgcagcca ctgtggctgg aagttgctga ttcccgaaac agtcattgcc
     4741 ggattgctta tcgacaccct gttttcattg atgccgttgc tcagcgaata gggacggacg
     4801 aacatggaat gttcggaatc atgtgcaagg aatacaaatc cttcacccct gtttttcctg
     4861 ttcagtatgg caaagtagtt gttggccatt gcattgccgt aaaaagctga caccggggcg
     4921 tcaaaattgc cggtatagaa gataaccagc atgtagtcaa tcaggttgtc aatatcaacc
     4981 cataccctgg tgtttttaac cgggttgccg ttctggtcct tcccttccag ggcgaaatag
     5041 tcagtgttgg atgcaaagcc acggttgcac cggttgtaga gctcctgcca tgatgccatt
     5101 gtaccgtcgg ttgcctcatt gaagtaaggc catgactcga cagatacctt gatgacgtca
     5161 tagtcgtcat ctccgtcacc gaaataggtt tcggcatagt cggcgtcagg ccgctcttcg
     5221 gtctggaaga ggccccagta catgccgttg aggtagaggt gataatattt tccccttgtg
     5281 taaggctgcc ccatgtcgcg ctgcgtatcc cgcgaaaaga tgtctctcag gaacgtatta
     5341 tgagtatcgc catccttgct ccatgaatag ttctgttctg ttctcaggtc aaccttatcg
     5401 aactcagcgg ctccttcgtt gccgaagagg ggatagagga gcttgccggg gccgtactcc
     5461 ttcctgaaga agagccggaa ggcgtgtttg ggattgtctg aagttctgct tgcacctccg
     5521 cgtatgcgaa cccccgcgtt tacccggaag cctatggtat tgtcaggatt aatgagctcc
     5581 agcgaacact gtctctccca ttcctcgcca tgctggtaag cgttgacata tatgcccccg
     5641 gaagcgccga acatatctgc aagatcagtc acgagtgata ttgaaggtat atccatcagg
     5701 cttgccttca tctgttgact gtacctggaa tcattcacca cgtcgggatc catcccgagg
     5761 tcaattacct gcccattgac cggggatgag ggccagcctc ctcccgggtg accctgggtc
     5821 agtaccttgt caggaaaaat gaatgacttt gtaacgggcc ttgaaggcga atagccctca
     5881 gtgaccagtg aggcacgcac gatgaatacc ggcgtggcag gacggcctgc cgatatgacc
     5941 ggatcaattc tcagcgtgac cgccgtgttg cttctgattg cagttgatga ggtctgcgga
     6001 ttgctgccat caatggtata gatgacatca tacccggact ggcctgatga gatggccaga
     6061 tcgaacggag cttcacagaa tccggcctca tggtcaaaat tcagcggcag tgtctccgga
     6121 acctcaggca gggagagtga cgcttctaca gccacgtcaa aataaaagtc ggaactgctt
     6181 ggtgacacgt tgaaggcctg aatacacagc aggtttgtcc cttcaaccaa tggcacatcg
     6241 gttgagtcaa ggtaaaaggt ctcaaatgta cccgactcgt ggtttgccac cgccagggca
     6301 ttgtaagaca gggtggccgg ggcgttgcgg gccatgaccc tggcaccgtt gacccatatt
     6361 acaaaaccgt cgtcataatt gaccctgaac cttatggttt taatcctgtc aatggaaaag
     6421 gcattgaatg aacttcgcag gtaaagggtg gagtacctgt tcatcatgtc agtgagtacc
     6481 actccgccaa ttccgtctcc gaagcggaaa ggggcgttcc cctcgctcca ttcagagtca
     6541 aagaattggg aagtcatcca gtctgccgaa aggctcgctg cgccggaacc tttaaggtac
     6601 ctgtacccgg aaccggaact gaattcaatc tgtcccgcgg cagcggaaca gattaggatt
     6661 gcaagactta ttgcgattaa cctcagacgc atctcttcac aacaatcccg ggcgtcaggc
     6721 tattctgcat cagcagcccg atccccgggg aaaatcaaaa gtagctcttt ttcctttata
     6781 cgccccgcgg cggaggatgt tgccgtatgt tgtgcagaat gtgaaagcgg aataacaaat
     6841 gatcagcgga gggctgcaca gaacagcgcc cgctcaggtg ccgcatattc ttaatgatcc
     6901 gactgtcacc ccggcagatg ccggcaggac acaatcagaa ctgaacatac tccacccttg
     6961 tgatcctcct ggctgccttg agggtatcct gcaaaaggtc actcaggtgg tgaagctcaa
     7021 attccttccc ccctttttcc tttgcataaa acttgctgag ttttcccggt gatgacctta
     7081 tcacaacgtt ccttgtgagg ttcatgaggt aggttctccc atgcggcagg ctcattatgt
     7141 gaaaattgta gtccatattg aatgaagtac ctgttgttag tcaaatcacg tcaacgttct
     7201 gcggatgccg gctaccggaa tcagttacat ttccttcatc cgtaaatatt taacgttcgt
     7261 tatataaaaa gattgtttaa accccctgat tccggatacg aattgagaaa gccgggagtt
     7321 atatatgata taatgaaagt cgccttatca taaactgact gaaataagat gaacagggaa
     7381 cgtggccggc agttttactt tagtgtagtg gcggcactga tagtgctcat gacgccgtgt
     7441 atcaagggcc agaccgggaa tgaaccggga acagtgccgg gaagcgctga tgacagcctg
     7501 aagtgcgttg attacgtctc atggtacaaa gctgccatga atgagggcaa catcagcggc
     7561 gcgttacagc cctggaggca ggcgctggcc ggatgtcctt cagtttccga agagctttat
     7621 actgacggtg aactgatcta caggtcgctc tttgacataa caggcgaaca gaagtacatt
     7681 gactccatgg tgatgatcct gacgcagaga acctactttt ataacaacaa gccttcaaat
     7741 gatcttcaca aggcggaaat cctccttgaa ctggcgggag atgacccggc ataccttggt
     7801 ctctgttaca acatccttgc tgaagctgca gggagtttcc ccgaacagat ggaatgtcat
     7861 cattttgtac agatggctgc cctggcggca agcctttatg cgatgggggt gattgacggt
     7921 gaagagctgg gcaatgcctt tgtcacagca ataggaaccg ttgactggcg gatggagaac
     7981 cacattgccg gctgcagtag tgcggatgat ctggagaata tggaaacctt ttatcgcacc
     8041 aacggtgcga tgacctgcga agggcttgag acgctctatg gcgagaagct cgaccgcaat
     8101 ttcagggata cggcttttgt cagcaaggtg tttgcgatga ttcaggaggc aggatgcacc
     8161 cggtcggatc tgtattacaa tgttgcagta aagatgtttg ccaatgaccg gtcagcacaa
     8221 aacgccgtca ggctggctga actgaacgct gccgccggca ataatgacaa ggccatttcc
     8281 tacttcaccg aggcatataa ccgtgacaca gacagcattg ttcgttctga agttctcatt
     8341 cgtgttgcga tgatggagct tggccagggg aaaagaccgg aggccaggga ccgtgcagag
     8401 catgcatggc agctgaacag gaaaaacgct gcggcgctga tgctcctcgc agagtgctat
     8461 gccggcgccg agctgggcaa cacttttgac aatcacactg cctactgggt ggcggccgac
     8521 tatcttgatg cagccgttaa ggctgatcct tcactgagga aggaggcaga aagaaaaata
     8581 aaggtttaca tgcagaattt ccccacgagg gaggagtgtt tttaccgcaa gatccttgac
     8641 gagggagtca tcttcactgt tggtggctgg gtaaatgagg taacccgcgt aaggttcagg
     8701 aaagagtaat acggaaaatc ccgggggaga aaattccggc agattcccgt aaacggaagg
     8761 tcagaccctg ccctggcagc aggatgttat ttttcagtgt tcagtttttg gaaactgtca
     8821 ctgtgataat atcacccgtc tcaataactt catattttac acctatcaga ttggcaatct
     8881 gtttcgcata tacttcataa aactccgata attcagccct tgtgatcttg aaatcatcga
     8941 gcattgcgtc aagcggttta taaaagttag ttaccgaaaa tgccacactg tccggagact
     9001 gttcaagtat ttccacttta cccgacgggg tcagaacaac gtaggtgtat accatgctgt
     9061 tcaccagatc atcaaatgtc atgtcgctgt tccatgttgc tgcagcaata ccgcccgctg
     9121 ctattgcagc ctcttcgaca gtctgcccga gtgatttggc atattttatc agtatcacat
     9181 atgcaccggt ccactgccat gctgcaaggt tgtatttctg aagatccgac ggagccggga
     9241 cagtaaattt atcctgtccc caggttgcca gtgcaagcat gcaacccaca atcagtacta
     9301 ttagcttttt cattttcaat ggattttgat atgttcaata attgatacgt ttgtcctgcc
     9361 tgtgataact tccttgaaat gattcatatc tctatctgtt ccagatattg agtactattt
     9421 tccattcacc gttgatcttt ttccagatgt caacccattt tgccgggtca tcaacaatcc
     9481 cgtcaggagt ccggttctga agcaggtcgg taccccagac ataagccatg tctcccgatg
     9541 atgagacttc aattaacccg atctttgttg aataggtttc ccacagacag gcagtgtctg
     9601 ccaattgcga tccaattctg ccgcgataat cttcaatacc tgtctcagga ggtacgccgg
     9661 gactcaggtg caagatgtca gacgttccat aactcagtag cttttcaatg tcgcgattct
     9721 gataggcgac agtcatctga ttctcaagat tacgcactgc ttctgcctcg gcagtgatgt
     9781 caacctttgg cgcagtggtg catcccgcca atagcatcag agccagaatg aacgggggaa
     9841 tttttttcat gatctgaagt tttaggataa atattccgtt taagaactca gatcaaagtt
     9901 acgccggtaa tttgctgaag tcaaatataa taagtgccag tcagccctga ggaatcatgt
     9961 tttggccggg tccatgaggt aacccgcgta atggtcagga aagagtaata cggaaaattc
    10021 cgggggagag aattccggca ggtctatctc cagacctggc ccaatgggat gatcttcatc
    10081 aggcatatgt catccccggc ctgatacgct gaatcggatt caaatctcag aaattaaccc
    10141 ttcccgaacc ccctttggtc aaaaacggct tttaaggcgg atttcaagcc tgcttcatct
    10201 aaaatactgc atatgaatgg cataaatggt catgtgcaca acgtaacacg aaagttcacc
    10261 aatttatggt aaaaacggga ctctttttga cgaatcgttt ttttccggtt accagcctgt
    10321 ctgaaccggg ggtgagaccc cctgaaaatt tgacaagcag ctcgcgtaac tgacacataa
    10381 tcacttattt cgcgacatgc gcccttgtct tgcgggggtt tctgaagtaa ctttgtcacg
    10441 aagatcaaaa cgaacagcca tgaagacatt cagacagatt tcttcaggtt taggcgttgc
    10501 ggccggatgc aatacagtac taaggaatct aaagctcaga ctgacagcaa aaagtgttgc
    10561 tccgaaggtc attttaatat tcatgacctt tttccttaat acgtatgccg ttccgggcgg
    10621 aaaacctgaa atgacggagg aaatgcaact ccgtgccgag aaggatctct tctatttcca
    10681 ggtcagatac cttcccttca gcgaggagtt gcttataaaa tgcctcgggt acgaaggcat
    10741 aaaataccag gaggtagtct tgctgcagtc gcgtcttgaa actgggtatt atacctctga
    10801 tattttcctt aacggcaaca accttttcgg tatgagatac ccggccttca ggcccacggt
    10861 agcaaccggc acctataagg agcatgcgcg gtacgcacac tggtctgatt cagtgatcga
    10921 ctatgcgctg tggcaggaat attacatgtc cagaggctac cggataggtg aggggcagga
    10981 cactgacttc tacctggtat ttctgaaatg catcccttat gccgaagaca aaatgtatgt
    11041 ttccaagctg gtgacaatgt caggtggaga tattacatag gcacattgct gtgaaactgt
    11101 tccttccgtc aacagtatca ttgtggtttt cgtattttta cgttggaaga aagtattcac
    11161 agtcaaaatg tcattgtcat gaaaagaatt gttgcggttc tgttattcag cctgatcctg
    11221 ttctcatcct tttgccagag ccagggtgtt gagctctctc attacgtatt tcctgatttt
    11281 acgaacggct ctgttctgat gaaatcaggt cagcagcatc aggctctgct gaactacaat
    11341 gccttatccg aggagatgat ttttgtcgac aaaggcacga agctggctat aagcaaggag
    11401 gagaaggaga agattgacac cgtttacata aagggaagga agtttttcgt gcttgatggc
    11461 cgtttcctcg aactgatata ccgttcagga tacgagctgt acgcggaaca caggtgcgat
    11521 gtgaaatatc ccggcaaacc ggccggatac ggaggcactt ctgaaacctc atccatatct
    11581 acatactccg gtgtatacag cggggggata ctgtatgaac tgagactgcc tgacgatttc
    11641 agcataaaac catacctcat ctactggctg aagaaagacg ggaagattaa taagattgtc
    11701 aatctcaagc agctgaataa agtattcggt gacaggaagg accagattaa ggagtatgtc
    11761 ggcagcaata agactgacta caatgatatt gagagcatgg tcgggctgat caggtacctg
    11821 gaagctaact gacgggattt accgcttttt tcagcagcct gagatacaac gctgcataca
    11881 aaagaagtga tgcaagccac aggactatta ggttgaacca cagagtcccg atctccgcat
    11941 tacctgtctt tttgtacgga gcatagaaat gggcacggcc gtaatccgaa acaggtttca
    12001 tgtatgccgg ctcgtatttc tggatgtacc tgcccctggc atccacaatc ttgtgttcgc
    12061 cgaactcatc aagcactatt tccctgagcc tcctgtttgt attgttttcc ttcagctcga
    12121 tgaatgcctg cttccccatc gaagccacaa gggccgccgt gacagaatcc tttttaatga
    12181 cgtttgcaat cctgacagcc ttgaaatggc ctgcgaggct gtcaagataa tgttcggcat
    12241 attcagccac ggcgggattg aatttttccg gactgagcga tgaaatcagc tccgcaggag
    12301 gaggatcaaa tcctgcaagt tccgaaagcc tgtggaaata ttttcccagc ctgttcaggt
    12361 tatcacttac ctggccggaa tattcagggc tgtcaccaaa aagaatacat tccctgatat
    12421 ccctccggag agccacagcc agaaaagcgg aataccagtt gttctgactg atctccgtat
    12481 caaaatcaaa gaagttgttc tcgtatttat tgcttctgta ctgttcaacg acaagtgctt
    12541 cgtacgccca cctggcgggc atgagttcac ccagaaccgg gacatattca ttttgtttat
    12601 cctcactgcg gtgaagactg tcaaacttca ccagcacccc gctgaacaac agctgtggaa
    12661 taattataaa cgggatcaga atatagatcg tcacaactga attgaaggcg gatgagatgt
    12721 taagacctgt caggctggca agggcggatg cgctgaacag gatgatccag taggtgaggg
    12781 tcatcccctt gataccaaga ataaggtttc ccgcgagaac aaatgttccg gtctggatgg
    12841 cggcaaacag gaacatcatt attatcttgg aattgatata gctgaaccag cttagattaa
    12901 ggaaagattc cctcttcagt atcctgcggt ccctgactat ctcctcggca ctcaggatca
    12961 gtcccatgaa aaaagcagta atgacacaca tgaacatata agccggaagg ttttcattgt
    13021 cactcaacct gtatgcctgg cccgagacgt tcctcgtaaa gaatgcaagc agaacggcaa
    13081 gcaggggagc tcccagcagg gcaatcagca tatactgcct gtccgcgacc tttgaaagga
    13141 gatctctttt aaaaaacaac agtgcctgtt tcgccggtcc gggtattgaa taattgcttc
    13201 ccggcaaagg ttctctggca ataaccctgt cattctcctt gttgcctttc tccagggtga
    13261 agttttcagc ccattccgca ggtgagactc ttcttacggg tgttggcttg ccaaactcat
    13321 tgaccaccct ggtctctatt atcctgagaa gctggtctgt atcaacatgg ccgcaggcga
    13381 tgcactgatc ttcaaagggg ttggccaggc tggccctttt cctgaaccat actacggtct
    13441 ctgtgggatt accgtgaaac acaagataac ctcccttatc cagtatcata atccggtcaa
    13501 acatcttgta gatgtctgac ccgggctgat ggatggtggc aattacaagc ctgcccctgt
    13561 aagcctgttc cttaagcatg ttcatgacag tctcggcatc aaccgaagaa agtcctgatg
    13621 tgggttcatc cacaaacagt atggttggtt cacggatcag ttcgagggct atattaagtc
    13681 ttttgcgctg gccgccgctg atgaccttgt ttaccggact gccgaccttc aggtccctga
    13741 cagagtacag atccatgtca gcgagtatcc tgttcacttc ctcgcatatc ctttccctgc
    13801 ccaggttgtt caggcacatc cgtgcattgt aataaaggtt ctggaatacc gtaagctcct
    13861 caaacagcag atcatcctgc ggaacgaacc cgatcacgcc tctgaggtgc tctttgccgt
    13921 cgggggtgtt caggtcatgt ccgtttatga agacattgcc tgaatccggc cttgcgattc
    13981 cgctgaggag attcagcagg gttgatttac caacgccgct gcctcccata ataccaacaa
    14041 gccttcctga ctcctcatgc agagacagtt tctgaactcc gttttcactt tccctgaacc
    14101 taaggctgat atcacgggca tcaagcgaga cctttaattt gaaggcttcg cccacgaaga
    14161 cctcttccac atcgttgaaa taaaccgggt tgatgccggc tcccctgaca gatgatccgt
    14221 gttcgaacac gtatgtctgc ccggagaaga ttatctgtcc gttgaggaga aggttttccc
    14281 tcccttcata acgcatcagg agggtgtttg tgctggcaat attgaggaac agcagcctgc
    14341 ccctcaggtt ttcgttatag aggtgcttga ttccctggct gtcatgggca gagtcgctgc
    14401 tgatcaccag cagccgctct ttccggaatc cttcggagcc cggtacgagt ataaatgcca
    14461 gcatgtcacg gtactcgccg gtctcaacat taaaggctgt ggccactgtt tcgaggaact
    14521 ccagttcctt gccgggtgcc ctggtgctga aggagatgaa atccatcagt tgaaccagca
    14581 catatatctt ctgcttctgc tgcagttcct cgtttatctt ctcacagatg gccagtatcc
    14641 gcattgcagt aagtgaagtc cgtttcctgt cacggatgct gtctttcgat aatacatcag
    14701 ggtggtaaag
//
LOCUS       DAOPXU010000067        14683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_4442_length_14683_cov_11.134947, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000067 DAOPXU010000000
VERSION     DAOPXU010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14683
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4442_length_14683_cov_11.134947"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PKK03_07965"
     CDS             complement(<1..202)
                     /locus_tag="PKK03_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84375.1"
                     /translation="MTIGKFRIHREGYKIIGGVLLGLAALNIAGWLVWPGPTICHWLA
                     ILASLLLFVFIVMFFRAPARPLE"
     gene            complement(204..1046)
                     /locus_tag="PKK03_07970"
     CDS             complement(204..1046)
                     /locus_tag="PKK03_07970"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNX84376.1"
                     /translation="MTNFVRRTVTGAFIVIFVLGGLWLHPVSFFLVGAVMIAGTQREY
                     YIMVRGTGVRPQLITGIISGFLLYVISTTVALGWLPRSWFLAMIPVISIVMVIELFRN
                     VERPFDSLAHTFFSILYTAVPFSMFPFSAFNQTGLSPLIPMEGIAFSPGIMVGFFLLL
                     WTNDTGAYLVGSALGRHRLFERISPKKSWEGFVGGLILTLLVARLFSGWLGVADTKGW
                     MAIALIISVGGTFGDLLESMLKRSVGLKDSGTILPGHGGFLDRFDSVVVAFPLVYLYI
                     ATML"
     assembly_gap    1087..1186
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1203..1400)
                     /locus_tag="PKK03_07975"
     CDS             complement(1203..1400)
                     /locus_tag="PKK03_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84377.1"
                     /translation="MENEWVAIAGFTDDVRSQIAVERLMTNGVDAVAIDKRDSIYKIG
                     EIEIFVHRDNVLIAKEIIKDL"
     gene            complement(1390..3525)
                     /gene="ftsH"
                     /locus_tag="PKK03_07980"
     CDS             complement(1390..3525)
                     /gene="ftsH"
                     /locus_tag="PKK03_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNX84378.1"
                     /translation="MAESNTPKSNKNEKSNGKSWFNSSWLFAILLLTFIVFQVLTSGK
                     YTQKAGQREIEAMVMHHDIEKVVIVNEEQAEIYLFPDSLKSGRYPDFEEQRSFGLQPP
                     KPHFYHTFGTVELFQKFFEEAQDKAGYTDDQRIYPDYQKRKDYLGTFLGVILPFILIA
                     AVWIYFMRRMSSGAGGGAGNIFSVGKSKAQIFDKDTHIKINFNDVAGLEEAKTEVMEI
                     VDFLKNPKKYTSLGGKIPKGALLVGPPGTGKTLLAKAVAGEANVPFFSISGSDFVEMF
                     VGVGASRVRDLFKQAKEKAPCIVFIDEIDAVGRARGRNPNFGANDERENTLNQLLTEM
                     DGFGTNSGVIILAATNRADILDKALLRAGRFDRQIHVELPDLKEREAIFKVHLRPIKL
                     EEGFDIAFLAKQTPGFSGADIANVCNEAALIAARGNKTMVQKQDFLDAIDRIIGGLEK
                     KNKIISMDEKKTIAYHEAGHATVSWLLEHASPLLKVTIIPRGKALGAAWYLPEERSIS
                     TREHILDELAYALGGRAAEELVIGKISTGALSDLEKVTKQAYAMVAYFGMSDEVGNIS
                     FYDSSGQSEYGFTKPYSEKTAELIDSEAKKIVEESYLKAKEVISSNIAGLTQLAEQLL
                     EKEVIFSEDLERIFGKRRGDKHAEAAAAEAATAGSSAGEGGVAGASATEAATAGSSAG
                     ESGVTGSQAREAEAPAAPATEVPGPGNGE"
     gene            complement(3534..3917)
                     /gene="rsfS"
                     /locus_tag="PKK03_07985"
     CDS             complement(3534..3917)
                     /gene="rsfS"
                     /locus_tag="PKK03_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNX84379.1"
                     /translation="MRKGTKVATGVTDSIVRGLLEKKGEKVALIDLRNIESRICDWFV
                     ISHAASTRQVDSLAWSVEDVVRKETGVKPYHIEGRENCIWVLLDYGDILVHIFQKPYR
                     EFYDLESLWADGRITYLDDNMEKKG"
     gene            4088..4828
                     /locus_tag="PKK03_07990"
     CDS             4088..4828
                     /locus_tag="PKK03_07990"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNX84380.1"
                     /translation="MIVGTYVRHFEKVSSTNAVAAAMLNEEAPAEGTVITASYQESGK
                     GQPGNSWESESGKNVIMSVILYPVMVRPADQFIISRMVSLAVYDLVSKHTRNASIKWP
                     NDIYVRNDKIAGILIENSIMGDTIGSSVSGIGLNVNQVVFRSGAPNPVSLKQVTGTTH
                     YLKAVKEELIAALDRRYAMVISGETAALENEYQRVLYRAGEWHRYSDDSGEFEGMIER
                     VMADGMLSVRRRDGTCRLYAFREIDYIL"
     gene            complement(4803..5264)
                     /locus_tag="PKK03_07995"
     CDS             complement(4803..5264)
                     /locus_tag="PKK03_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84381.1"
                     /translation="MGNRDMSPMTTFTSRRGEVPCGDSDLYAFLTDMRNFQTVLPSGS
                     VTDWEATEDRCSFKTDGAGRITVALSEALPHSEISYEAESFLTGKVKVRITIEFISGM
                     RSAVRVDTGLNMNPLLRMLIGDSAGRYLEMLMDIIESYDGYDRVRGCNQSP"
     gene            complement(5246..5884)
                     /gene="pyrE"
                     /locus_tag="PKK03_08000"
     CDS             complement(5246..5884)
                     /gene="pyrE"
                     /locus_tag="PKK03_08000"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNX84382.1"
                     /translation="MDETARKTAEYLLQIKAIKLQPAKPFTWASGWKSPIYCDNRMTL
                     SFPEVRGFIRDSFAARVRELYPGADLIAGVATGAIAHGALAADALGMPFVYVRSGAKD
                     HGLGNQIEGRYEKGQKVVVIEDLISTGGSSLGAVRALRDAGCEVLGMIAIFTYGFAKA
                     VDAFRAEGCRLDTLSNYSMLVDLAAASGYISHGDVETLKEWRKDPASWGTGI"
     gene            5885..6502
                     /locus_tag="PKK03_08005"
     CDS             5885..6502
                     /locus_tag="PKK03_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNX84383.1"
                     /translation="MVKYKVYFDNRVITLSPEPDRVQKYTLYHKYNQPEELFTQISQF
                     AVNNEIPSLNIYSLDINNLWKVFSSWFVVINAAGGLVGHPSGRYLFIMRYGRWDLPKG
                     HMEENETPEECAIREVTEECNIQGHIIEGPLPPSYHTYLFDNKPYMKKTWWFRMSYRG
                     EMITDPQASEGITHAEWLAPDEIARIKSNTFQSLLDLLNFTLRVK"
     gene            complement(6598..7074)
                     /gene="coaD"
                     /locus_tag="PKK03_08010"
     CDS             complement(6598..7074)
                     /gene="coaD"
                     /locus_tag="PKK03_08010"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNX84384.1"
                     /translation="MDKVALFPGSFDPFTVGHESLVKRGLQLFDRIVIAVGENADKKN
                     LFSIEMRMKMISRVFEGENRIEVTRYSGLTVEFCRSCGARYILRGLRTAVDFEYERAM
                     GHMNWKMAPEIDTVFLLTSTEHTPINSTIVRDIIRNGGDVSMFVPAVINISDYLGK"
     gene            complement(7090..9552)
                     /gene="lon"
                     /locus_tag="PKK03_08015"
     CDS             complement(7090..9552)
                     /gene="lon"
                     /locus_tag="PKK03_08015"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNX84385.1"
                     /translation="MEKKVYGKWDRIGLHELIDEGREIIPILSDGDDQELEEVQIPGS
                     VPLLPLRNTVLFPGVVIPIAVGRSKSVQLVRDAYRGDKLVAAVAQLDAEVDDPGVRDL
                     NVVGTLGHVVKLLEMPDGTTTAIIQGRKRVAIDNIIQTTPYMTAQVRAMPEPRGVDDE
                     KDFEVIIGSLKDLSVKIVKLNPNISPEATFAVRNIENSTYLINYICANTEINVTDKQK
                     LLEANSIRERGLRLLEHLVREIQLLELKNELQSKVKYEIDQQQREFLLHQQMKTIQNE
                     LGGNPIEKEVEEFRARGAKKAWGEDVAKVFSRELDKLSRLNPAAAEYSVQVNYIQTLL
                     ELPWNEYTTDNLDLKNAQSVLDEDHYGLEEVKERILEHLAVLKLKGDLKSPILCLYGP
                     PGVGKTSLGKSVARALGRKYARMSLGGLHDEAEIRGHRKTYIGAMPGRVVQNIRRAGS
                     SNPVFILDEIDKVGHDFRGDPSSALLEVLDPEQNTHFYDNFLELEFDLSKVLFIATAN
                     TLSTVSPALRDRMELIEISGYLVEEKREIAVRHLIPKQLEAHGVTPEQFLLSREVIEE
                     VIQKYTRESGVRELDKRIAKLVRFRAREIAFENRPEPEVSSAMVEEILGPPKYLKDIY
                     DGNDQAGVVTGLAWTATGGEILFVETSLSRGKGNLTLTGNLGEVMKESAVIALEYLKA
                     HASLLSLPDTFAEKWNIHIHVPEGAIPKDGPSAGITMAVSIASAFTQRKVKKYVAMTG
                     EITLRGKVLPVGGIKEKILAAKRAGIKEIVLSEQNRKDVEEINEKYVRGLKFKYVSTI
                     MDVLDHVLLQQKVANPRVIEFE"
     gene            complement(9690..9956)
                     /locus_tag="PKK03_08020"
     CDS             complement(9690..9956)
                     /locus_tag="PKK03_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HNX84386.1"
                     /translation="MKEGIHPNNYRLVVFQDMSNGFTFMTRSTAGSRDTIKWEDGKEY
                     PLIKLEISNTSHPFYTGKMKLVDTAGRVDKFMNRYKDHLTKKTK"
     gene            complement(10054..11262)
                     /locus_tag="PKK03_08025"
     CDS             complement(10054..11262)
                     /locus_tag="PKK03_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84387.1"
                     /translation="MKITEVTNEFLRKEFIEFPKKLYKGDPNWVCPLDRETEAVFDHE
                     RNAAFRHGEAVRWILSDDDGTTIGRVAAFIDEIRSKANRQPTGGLGFFEVIASREAAF
                     ILFDTARQWLTERGMKAMDGPINFGENDNYWGLLVDGFIQPGFGMPYNKPYYKEYFTG
                     YGFRTYFEQYSYHKDVAAVEVFPERFRKIAEWISKKPGYTYRHFDFSQRDKFAADMVE
                     IYNATWAVFKEDFTPLDPARIENTLHQASAFIDPELIWFAYHNDRPVAFFIIFPDLNQ
                     IIRHFNGRLTLLNKIRFLWYKMTHEMTRARALVAGVHPSYQNTGIESAIFLELFRVFR
                     KKRYYRELELSWVGDFNPKMISIYEAIGATKAKTHLTMRYMMDETLPFKMYKDEMEEN
                     RAARQARQKE"
     gene            complement(11268..12755)
                     /locus_tag="PKK03_08030"
     CDS             complement(11268..12755)
                     /locus_tag="PKK03_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNX84388.1"
                     /translation="MAFHDSGLEDYFGTFRRNIVGIHQDYTSPYGKKKIIYCDWIASG
                     RLYEPIEEKMSHLFGPFVGNTHTETSETGSMMTWAYHHAQEIIKKHVNAGPDDVLIAA
                     GAGMTAVINKFQRILGLKGCSYPRTARCLGDTDRPVVFITHIEHHSNQTSWYETLADV
                     VIIEPGSDLTVDPENLKKALEKYSDRKFKIGSFSACSNVTGVFTPYHTLARIMHEHGG
                     LCFIDFAASAPYVDMNMHPDDPMEKLDAIFFSPHKFLGGPGSSGIMLFDKSLYKSSAP
                     DQPGGGTVDWTNPWGEYKYVDNIESREDGGTPAFLQMIRAALAIRLKEQMGTAKIRAR
                     EEELVARTFSGLSAIPGLHILASDIRHRLGAVSFYIDGLHYNLVVKLLSDRYGIQVRG
                     GCACAGTYGHYLLDVTYDHSHRITEMINKGDLSEKPGWVRLSVHPTMTAAELETVIAA
                     LAEIRDNATEWSNDYIYCRRTNEFTHRDQTGAGMERVSSWFNLQT"
     gene            complement(12843..14189)
                     /locus_tag="PKK03_08035"
     CDS             complement(12843..14189)
                     /locus_tag="PKK03_08035"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HNX84389.1"
                     /translation="MSRLKIIDNISGLQAVQLLRFLTFLIISIVFTKSHLSRAQIGNW
                     ELFLFISSLLSFFWVTGIIQSLLTLYERNRTFRDDGAREGRKSPEIYNAFLLISFFSL
                     MVFILGIPIRSVLESSPADPDIPYPNLLLLYILLSNPVALIEYIYLLNKRPHRILQYG
                     IYTYSAQLLLVLAPVVAGYDEKSAIWGLFAITAVRWIWLIILLRRYTLLKVSPGFIRE
                     HLRVGMPLILTFLISGSAQYVDGIIISARYSDPGVFAMYRYGAKEFPLVLLLANGLSN
                     ALLPQFSTREGMREALVTLRKRSDRLIKYLFPLSMVTMLVARWVYPRLFTPEFTRSAD
                     VFMIYLLLIIPRLVFPQTIVIGRRKTNVTLYAAAIEIAVNIPLSLMLIKPYGVVGVAL
                     ATFIVYFLEKIFLMYYVWKKMKIRPAEYIPLTRWIIFSALLILLFVLIDHRIIDIY"
     gene            14346..14421
                     /locus_tag="PKK03_08040"
     tRNA            14346..14421
                     /locus_tag="PKK03_08040"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14381..14383,aa:His,seq:gtg)
BASE COUNT         3349 a         3913 c         3659 g         3662 t
ORIGIN      
        1 gctcaagcgg cctggccggt gccctgaaaa acatcacgat gaagacgaac aggagtagtg
       61 acgccagaat ggccagccag tgacagatcg tcgggccggg ccataccagc catccggcga
      121 tgttcagggc agccagccca agaagcaccc ctccgattat cttataaccc tccctgtgta
      181 tcctgaattt tccgatagtc atatcaaagc atcgttgcaa tgtaaagata aaccagcggg
      241 aatgccacca ccacgctgtc gaacctgtca agaaatccac cgtgccccgg caagatagtc
      301 cctgagtctt tcaatcccac gctccgcttg agcatcgact ccagcaggtc accaaacgtt
      361 ccccctacgg agatgataag tgcaatggcc atccaccctt tggtatcagc cacaccgagc
      421 catccggaaa agagccttgc caccagtaat gtcagaatga ggccaccgac gaatccttcc
      481 catgatttct ttggtgatat gcgttcaaac agacggtgcc tgcccagtgc ggatccgaca
      541 agataagcac cggtatcatt tgtccataga aggaggaaaa aaccaaccat gattccgggt
      601 gaaaaagcaa ttccctccat cggtatcagc gggctcagcc ctgtctggtt gaaagcagag
      661 aaggggaaca ttgaaaacgg cactgcagtg tacagtatcg agaaaaaggt gtgcgccagg
      721 gaatcaaaag gccgttcgac attgcggaaa agctcaatga ccattacaat tgatatcacc
      781 gggatcattg caaggaacca gcttctcggc agccatccca gcgccaccgt ggtagagatg
      841 acatagagga ggaatccgct gattattccg gtgatcagct gtggccgtac cccggtaccc
      901 ctgaccatga tgtaatactc gcgttgtgtg ccggcgatca tcactgctcc gacaaggaag
      961 aatgacacag ggtggagcca cagtcccccg agaacgaaaa tcactatgaa ggcacccgtg
     1021 acggtgcgtc tgacaaaatt tgtcacacta cagatccttg attatttcct tagctatgag
     1081 cacattnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngaca aaatttgtca
     1201 cactacagat ccttgattat ttccttagct atgagcacat tgtcacgatg gacaaaaatc
     1261 tcgatctctc ctatcttata tatgctgtca cgcttgtcta tcgccactgc atcgactccg
     1321 tttgtcatca gcctttcaac ggcaatctgg cttctgacat catcggtgaa gcctgctatg
     1381 gcaacccatt cattctccat taccgggacc tgggacttca gtggcgggag cagctggggc
     1441 ttcagcctcc ctggcctgtg aaccggtaac tccgctttct cctgcagatg aacctgctgt
     1501 ggcagcctca gtagcagagg caccggctac tccgccttct cctgcagatg aacctgctgt
     1561 ggcagcttcc gctgccgcag cctcggcatg cttatcacca cggcgcttgc cgaagatacg
     1621 ctcgaggtcc tcactgaaga tgacctcctt ctcaagcaac tgctcggcaa gctgggtcaa
     1681 cccggctata ttggacgaga tgacctcttt agccttcagg tatgactcct cgacgatttt
     1741 ctttgcctcg ctgtcaatca gctctgccgt cttctcgctg tatggcttgg tgaagccgta
     1801 ctcactctgg ccggaggagt cgtagaagct gatgttgcca acctcatcag acatgccgaa
     1861 gtaggcaacc atggcgtatg cctgctttgt aaccttctcc aggtcactca gcgcgccggt
     1921 tgagatcttt ccgatgacca gctcctcggc agctcttccg ccaagggcat aggccagttc
     1981 gtcaagtatg tgctcacgtg ttgagataga cctctcctcg ggcaggtacc acgctgcacc
     2041 cagcgctttg cccctgggga tgatggtcac cttgagcagc gggctggcgt gttcgaggag
     2101 ccagcttaca gtcgcatgac cggcctcatg gtaggcaatg gtcttcttct catccatgga
     2161 gatgatcttg ttctttttct ccagtcctcc tatgatacgg tcaatggcat caaggaaatc
     2221 ctgcttctgc accatggtct tgtttcccct ggcagctata agcgcagcct cattgcacac
     2281 gttggcaatg tcagcgccgg agaatcccgg agtctgcttg gccaggaagg ctatgtcaaa
     2341 tccctcctcg agcttgatcg ggcggagatg caccttgaaa atggcctccc gttccttcag
     2401 gtcgggaagc tccacgtgta tctgccggtc aaaacggcct gccctgagca gtgccttgtc
     2461 aaggatatcg gccctgtttg tggctgcaag gatgatcact ccgctgttgg tgccgaaccc
     2521 gtccatctcg gtgaggagct ggttaagggt gttctcccgc tcatcattgg cgccgaaatt
     2581 cgggttgcgt ccacgggcac gccccacggc gtcaatctca tcaatgaaga caatacaggg
     2641 agccttctcc ttggcctgct tgaacaggtc ccttaccctc gaggcgccca ctccaacgaa
     2701 catttcaaca aagtcagacc ccgagatgga gaagaaaggg acgttggctt caccggcaac
     2761 agccttggcc agcagtgttt ttcccgttcc gggaggaccg acaagcaatg ctcccttggg
     2821 tatcttgcca cccagggatg tatatttctt cgggttcttg agaaagtcaa caatctccat
     2881 aacctcagtc ttggcctcct cgaggcctgc cacgtcattg aagttaatct tgatatgggt
     2941 atccttgtca aatatctggg ccttcgattt cccgacgctg aagatgtttc ctgcaccgcc
     3001 accggcgccg gaagacatgc ggcgcatgaa atagatccag acagcggcta tcaatatgaa
     3061 cggaaggatg accccaagga acgttcccag gtagtccttc ctcttctggt aatcgggata
     3121 tatgcgctga tcatcggtat atcctgcctt gtcctgtgcc tcctcaaaga acttctggaa
     3181 aagctctacg gtgccgaagg tatgatagaa atggggtttg ggcggctgca gtccgaaact
     3241 gcgctgctct tcaaaatcag ggtaacgtcc gcttttcagc gagtcaggga acagatatat
     3301 ctcggcctgc tcctcgttga caatgaccac cttttcaata tcatggtgca tgaccatcgc
     3361 ctctatctcc ctctggcccg ccttctgggt atacttgccg gaggtaagga cctggaaaac
     3421 gatgaatgtc agcaacagaa tagcaaacag ccagctggaa ttaaaccacg acttgccgtt
     3481 acttttttca ttcttgtttg acttcggagt gttgctctct gccataatat aaattaacct
     3541 ttcttttcca tgttgtcatc gagatacgtt atgcggccgt cagcccataa cgactcaagg
     3601 tcataaaatt ccctgtaagg cttctggaag atatgcacga gaatatcgcc gtaatcgagc
     3661 aagacccaga tgcagttttc cctgccctcg atgtggtagg gttttacccc tgtttccttt
     3721 ctgaccacat cttccactga ccatgcaagt gaatcaacct gtctggtgga ggcggcatga
     3781 ctgataacga accagtcaca aatcctgctt tcaatatttc tcagatcaat cagggccacc
     3841 ttttcaccct ttttctccag cagacccctg acaatgctgt cagttacccc ggtggccacc
     3901 tttgtgcctt ttctcatcaa taatcttttc agttataaac agttctgtaa gcagacgcaa
     3961 aattacgaac atttatccgt attttagtcc ggccttagct ctttttacaa tttgggtgcc
     4021 aatttgttca gaacggtaat taatgccgtt ttccgtcaga aaagcacccc tttgtcaggt
     4081 gattgtcatg atagtaggta catatgtcag gcactttgag aaagtgtcat ccacgaatgc
     4141 agttgcagct gccatgctga atgaagaggc acctgctgag ggtacggtga taaccgcttc
     4201 ataccaggag agcggcaagg gacagcccgg gaacagctgg gagagcgagt ccggcaagaa
     4261 tgtgattatg agtgtaatac tttatccggt catggtccgg ccggctgatc agttcattat
     4321 ttcaaggatg gtctcgctgg cagtttatga cctggtatcg aaacatacgc ggaatgcgag
     4381 catcaagtgg ccgaatgaca tttatgtgcg caatgacaaa attgcaggca ttctgatcga
     4441 gaacagcatc atgggtgata ccattggcag cagtgtatcg ggcatcgggc tcaatgtaaa
     4501 ccaggtcgtc ttccggagcg gtgcgccaaa tcccgtctca cttaagcagg ttacaggcac
     4561 aacccattat ctcaaggctg tgaaagagga gctgatagcg gcccttgaca ggaggtatgc
     4621 gatggtcatc agcggagaaa ctgccgcgct tgaaaatgaa taccaacggg tcctctaccg
     4681 ggccggagaa tggcaccgct attcagacga cagcggcgag tttgaaggaa tgatagagag
     4741 ggtcatggca gacggcatgc tatccgtcag gagaagagac ggcacctgcc ggctctatgc
     4801 gttcagggag attgattaca tcctctgacc ctgtcatacc cgtcgtatga ctcgatgata
     4861 tccatcagca tctcaaggta tcgtcccgcc gaatctccaa tgagcatcct caacagggga
     4921 ttcatgttca gcccggtgtc aacgcgcacc gctgacctca taccgctgat gaattcaatg
     4981 gtgatcctca cctttacctt accggtgagg aatgactctg cctcatagga tatctccgaa
     5041 tgaggcaatg cttccgacag agccacagtg atgcgtcctg ccccgtcagt tttgaaactg
     5101 cacctgtctt cagtagcctc ccagtcggtg actgaaccgg aaggaagtac cgtttggaag
     5161 tttctcatgt ctgtcaggaa tgcatagagg tcgctgtctc cgcacgggac ctctcccctc
     5221 ctgctggtaa aagtggtcat cgggctcata tccctgttcc ccatgaggcc gggtccttgc
     5281 gccactcctt cagtgtttcc acgtcgccgt gggaaatata acccgatgca gcagccagat
     5341 caaccagcat gctgtagttg ctgagagtat cgagtctgca tccttccgcc cggaaggcgt
     5401 caaccgcctt tgcaaagcca taggtgaaaa tggcaatcat tcccagcacc tcacatcccg
     5461 catcgcggag ggccctgaca gcgcccaggc tgcttccgcc ggttgagatc aggtcttcta
     5521 ttacaaccac tttctgccct ttttcatacc tcccctcaat ctggtttccc aggccgtgat
     5581 ccttggcgcc tgacctgaca tatacgaagg gcattcccag tgcgtctgcc gccagtgcac
     5641 cgtgcgcaat ggcacccgta gccactccgg ctatcaggtc ggcacccggg taaagctccc
     5701 tcaccctggc ggcaaaggag tcacggatga acccgcgaac ctccggaaag gacagtgtca
     5761 tgcggttgtc gcagtaaatg ggcgacttcc atccggaggc ccatgtaaaa ggttttgccg
     5821 gttgtaattt tattgcttta atttgcagta ggtattctgc ggtttttctg gctgtttcgt
     5881 ccatatggtg aagtataagg tatatttcga caacagggtt atcacactct cccccgagcc
     5941 tgacagggtt caaaaataca ccctttatca taaatacaac cagccggagg aactcttcac
     6001 ccaaatatca cagtttgcag tcaataatga aatcccctca ctgaatattt attcattgga
     6061 tatcaacaac ctctggaagg tcttttcctc atggtttgtg gttattaatg ctgccggagg
     6121 cctggtgggg cacccctcgg gccgctacct gttcattatg aggtatggcc ggtgggacct
     6181 cccgaagggt catatggagg aaaatgagac gcctgaagag tgcgccatca gggaggtgac
     6241 ggaagagtgc aacatccagg gacacatcat cgagggtcct cttccgccga gctaccacac
     6301 ctatctcttt gacaacaaac catatatgaa gaagacatgg tggttcagga tgagctacag
     6361 gggagaaatg atcactgacc ctcaggcatc ggaggggatc acccacgcag aatggcttgc
     6421 acctgacgag atagcaagaa tcaagtcaaa cactttccag tcgcttctcg atctgttgaa
     6481 ttttaccctc agggtaaagt aatcacacct gtgccgtatt ggtttacctg tgcagaacgg
     6541 ccggggctgt accgctgtgt caccgggcat gccgggaaag ctgtcaggca ggacatacta
     6601 ttttcccagg tagtctgaaa tattgatcac cgcgggaacg aacatggaaa catcgccgcc
     6661 gttcctgatt atgtcgcgga ctattgtgga gttgatcggg gtatgctcag tgctggtaag
     6721 gaggaacacg gtatcgatct ccggagccat cttccagttc atgtgaccca ttgccctttc
     6781 atattcaaag tcaactgcgg tacggagccc cctgaggatg taccgtgctc cgcaactgcg
     6841 gcagaattcc accgtaaggc cgctgtagcg ggtaacctca atcctgttct ctccctcgaa
     6901 taccctggag atcatcttca tgcgcatctc tatgctgaag agatttttct tgtcggcatt
     6961 ctcccctaca gcaatgacta tcctgtcaaa gagctggagg cctcttttta caagtgattc
     7021 atgaccgaca gtgaagggat caaacgaacc cgggaaaaga gcaactttat ccatggcctg
     7081 attatgctgt tattcaaatt caattaccct cggatttgcc accttctgct gaagcagcac
     7141 atggtcaagc acatccatta ttgtacttac atacttgaat ttcaatcccc tgacatactt
     7201 ctcattaatc tcttcaacgt ccttccggtt ctgttccgag agcactatct ccttgatgcc
     7261 ggcgcgtttg gctgccagga tcttctcctt gatgccgccc acgggaagta cctttcccct
     7321 cagggtaatc tcaccggtca tagccacata tttcttcact tttctctggg tgaaggccga
     7381 agcaatcgaa acagccatgg ttatgccggc ggaaggaccg tccttcggga tggctccttc
     7441 cggaacatgg atgtgaatat tccatttctc cgcgaaggta tccggcaggc tgaggagtga
     7501 ggcatgcgcc ttcaggtact cgagggcgat cacggccgat tccttcatca cctcacccag
     7561 gttaccggta agtgtcaggt tgcccttgcc gcggctgaga ctggtctcca cgaagagtat
     7621 ctcgccgccc gtggcagtcc atgccagccc ggtgacaacg cccgcctggt cattgccgtc
     7681 ataaatgtcc ttcaggtatt tcgggggacc gagtatctcc tccaccatgg cagagctgac
     7741 ctccggttca ggcctgtttt caaaggctat ctcgcgggcc ctgaacctga ccagtttggc
     7801 tatcctcttg tcaagctccc tgacgcctga ttcgcgagta tatttctgta tcacctcctc
     7861 aataacctca cggctgagca ggaactgttc cggggtcaca ccgtgcgctt caagctgctt
     7921 gggtatcagg tgcctcacag ctatctcccg tttctcctca accaggtatc ctgatatctc
     7981 tatcagttcc atcctgtcac gcagggccgg gctcactgtt gagagcgtgt tggctgtggc
     8041 gatgaacagt accttcgaga gatcaaactc cagctccagg aagttatcgt agaaatgtgt
     8101 gttctgctcg gggtcaagca cctccaggag ggccgatgac gggtctcccc tgaaatcatg
     8161 tcccaccttg tctatctcgt caagaatgaa gacggggttt gaagagcctg ccctccttat
     8221 gttctgcacc acccggcccg gcatggctcc gatgtaggtc tttcggtgcc ccctgatttc
     8281 cgcctcgtca tgcaatccgc ccagtgacat gcgagcgtat ttgcgtccca gcgcccgggc
     8341 aacagacttg cccagcgagg tcttgcccac tcccggcggg ccgtaaagac ataggatagg
     8401 cgacttcagg tcgcccttca gcttcagcac ggcgaggtgt tccagtatcc tttccttgac
     8461 ctcctcaagc ccgtagtgat cttcatccag cacgctctgg gcattcttaa ggtcaaggtt
     8521 atcagttgta tattcattcc agggcagctc aagcagggtc tggatgtagt tgacctgcac
     8581 cgagtattcg gcggcagccg gattgagacg tgacagcttg tcaagctcac ggctgaagac
     8641 cttcgctaca tcctcacccc aggctttctt cgcgcccctg gcgcgaaact cctcaacctc
     8701 cttctcgatg ggattgccgc ccagctcatt ctgaatggtc ttcatctgct gatgaagcag
     8761 gaactcacgt tgctgctggt caatctcgta ctttaccttc gactgcagtt cgttcttcag
     8821 ctcaagcagc tgtatttcac gcacgaggtg ctcaagcagc ctcagcccgc gctccctgat
     8881 cgagttcgct tccaggagct tctgcttgtc agtgacattg atctcagtgt tggcgcagat
     8941 ataatttatc aggtaggtgc tgttttcaat attcctcacg gcgaaggtgg cctcgggact
     9001 gatattcggg ttcagcttga cgattttgac agagaggtcc ttcagtgatc cgattatcac
     9061 ttcgaagtcc ttctcatcat ccactcccct cggttcgggc atggccctca cctgcgccgt
     9121 catgtatggc gtggtctgga taatattgtc tattgcaact ctttttctgc cctggattat
     9181 ggcggtggtg gttccgtcag gcatctcaag gagttttacc acatggccca gcgttcccac
     9241 cacattcagg tcgcgtaccc cggggtcatc cacctcggcg tctagctgtg ccactgcggc
     9301 aaccagtttg tcaccccggt aggcgtcgcg cacaagctgc accgactttg aacgccctac
     9361 ggcaatgggt atcacaaccc ccggaaagag aacggtgtta cgaagcggca agaggggaac
     9421 actccccgga atctgaacct cctccagctc ctggtcatca ccatccgaaa ggatggggat
     9481 tatctccctt ccctcatcaa tcagctcatg caaaccgatc ctgtcccact ttccgtaaac
     9541 ctttttttcc atatggagta aaagtatgca aatttactta atattactct aactgtgcgg
     9601 gcatccgccg tatccggggg gcatatgcca tagacaaaca aaaaaaaagc cgccttgttt
     9661 aaggcagccc tgtatcatgt gactatcagt tacttcgtct tctttgtaag atgatccttg
     9721 taacggttca tgaacttgtc tacccttccg gctgtgtcaa caagcttcat cttacccgtg
     9781 tagaaaggat gtgatgtgtt tgaaatctca agcttgatga gcggatactc cttgccgtct
     9841 tcccacttga tggtatcacg tgatccggcc gttgaacgcg tcataaaggt gaatccgttt
     9901 gacatatcct gaaatacaac cagcctgtaa ttgttcggat gtataccttc tttcatctct
     9961 gatataattt attactgttc aaaaatccct ttttcaaggg agtgcaaaga taatcaacaa
    10021 taaagaaaat gcaaaaagag tattaaaaaa atttcactcc ttctgtctgg cctgcctggc
    10081 ggccctgttt tcctccattt cgtccttgta cattttaaac gggagggtct catccatcat
    10141 atatctcatg gtgaggtggg tctttgcctt cgtagcgccg atagcctcgt aaattgatat
    10201 catcttcgga ttgaaatctc ccacccatga gagttcaagc tccctgtagt acctcttctt
    10261 tctgaagacg cggaagagct ccaggaagat cgccgattcg atgccggtgt tctggtaaga
    10321 ggggtgtacg ccggcaacaa gcgccctggc cctggtcatc tcatgagtca tcttgtacca
    10381 gaggaaccgg atcttgttca gcaatgtcag gcggccgttg aaatgcctta taatctggtt
    10441 cagatccggg aaaatgatga agaatgccac aggcctgtca ttatggtatg caaaccagat
    10501 cagctcggga tcaataaagg cactggcctg gtgcagggtg ttctctatcc tcgcgggatc
    10561 aagcggggtg aagtcctcct tgaacactgc ccatgtggcg ttgtagatct ccaccatgtc
    10621 ggcagcaaat ttgtcacgct gtgaaaagtc aaaatggcgg taagtgtatc ccggcttctt
    10681 agagatccac tcggctatct ttctgaaccg ttcgggaaag acttccacgg cagccacatc
    10741 cttatggtaa ctgtattgct cgaagtaggt ccggaaaccg tacccggtaa aatactcttt
    10801 gtagtagggc ttgttgtaag gcattccgaa acctggctga atgaatccat ccaccaggag
    10861 accccagtag ttgtcatttt ctccgaagtt tatggggccg tccatcgcct tcatccctct
    10921 ctctgtcagc cactgccggg cggtatcgaa gaggatgaag gcagcttcgc gcgatgcgat
    10981 gacctcgaag aaaccgagac ctcccgtggg ctgcctgttt gcctttgagc ggatctcatc
    11041 aatgaatgct gccaccctgc ctatggtggt gccatcatcg tcagagagga tccagcggac
    11101 tgcctcgccg tgcctgaagg cagcattgcg ttcatggtca aagacagcct cagtttcacg
    11161 gtcaagggga cagacccagt tgggatctcc cttgtagagc tttttcggaa actcaatgaa
    11221 ttcctttctg aggaattcat tggtaacttc agtaattttc attgtcttca ggtctgaagg
    11281 ttgaaccacg agctgactct ctccattccg gcgccggtct gatcacggtg ggtgaattca
    11341 tttgtgcgcc tgcagtatat ataatcattg ctccattcag ttgcattgtc ccggatttcc
    11401 gccagggcgg caataaccgt ttcaagttca gcggcggtca tggtgggatg tactgacagc
    11461 ctgacccatc cgggtttttc tgacaggtca cccttgttga tcatctcggt gatccggtgg
    11521 ctgtgatcat aggtgacgtc gagcaggtaa tggccgtagg tgcctgcaca cgcgcatcct
    11581 cccctgacct ggatgccgta gcggtcactc agcagtttga ccaccaggtt gtagtggagg
    11641 ccgtcaatat aaaaggagac tgcgcccagc cggtgcctga tgtctgatgc caggatgtgc
    11701 agccccggta tggctgacag cccgctgaaa gtcctggcca cgagctcttc ctcgcgggcc
    11761 cttatctttg cggttcccat ctgttctttc agcctgattg caagtgcagc ccttatcatc
    11821 tgcagaaagg ccggggtacc accgtcttcc ctcgactcga tgttgtcaac atacttgtac
    11881 tcaccccatg gattggtcca gtcaaccgtg cctcctccgg gctggtcagg tgctgatgac
    11941 ttgtataatg acttatcaaa cagcattatg cctgatgacc ccggtccgcc aaggaactta
    12001 tgtggtgaaa agaaaatggc gtccagcttt tccatggggt catccggatg catgttcata
    12061 tccacatagg gtgccgaggc ggcgaaatca atgaagcaga gtcccccgtg ctcatgcatg
    12121 atgcgggcca gggtgtgata gggagtgaag actcctgtta cgtttgagca ggccgaaaat
    12181 gatcctatct tgaactttct gtcgctgtac ttttcaagcg cttttttcag gttctcaggg
    12241 tcaacggtga gatcgcttcc cggttcaatg attaccacat cggcaagggt ctcgtaccat
    12301 gaggtctgat tcgaatggtg ttcgatatgg gtgataaaga ccaccgggcg gtctgtatcg
    12361 cccaggcagc gtgccgttct ggggtagctg cagcccttga gccccagtat cctctggaat
    12421 ttgtttatga cagccgtcat gccggctcct gcggcaatga gcacgtcgtc aggcccggca
    12481 ttcacatgtt tcttgattat ctcctgggca tgatgatatg cccacgtcat catcgagcct
    12541 gtttcggagg tttcggtatg tgtgttgcct acgaagggac cgaaaagatg gctcatcttt
    12601 tcttctatcg gttcgtagag tctgccgctg gctatccagt cacaatagat gatcttcttc
    12661 ttaccataag gagaggtgta atcctggtgg atccccacaa tatttcttct gaatgtgccg
    12721 aaataatcct ccaggccgct gtcgtgaaaa gccatacttt taggcgttat atatgtccca
    12781 aatataattc tttttggcat tatggaagag ccgggcaccc ttccgcagtt tccgtaaagg
    12841 aatcagtaga tgtctatgat gcgatggtca atcagcacga agagcaggat gagcaatgca
    12901 gaaaagatga tccaccgggt aaggggtata tattctgcgg gcctgatctt catttttttc
    12961 cacacataat acatcaggaa tatcttttcg aggaaataga caatgaaggt ggccagtgcc
    13021 acgccaacca caccatatgg cttaataagc atgaggctga gggggatgtt cacagctatc
    13081 tcaatggcag cggcatagag tgtcacattg gttttgcgtc ttccaatcac gatggtttga
    13141 gggaatacca gccttggaat gatcagaagc aggtagatca tgaacacgtc agcgctgcgg
    13201 gtgaattcgg gggtgaacag gcgcgggtag acccatctgg ccacgagcat tgtaaccatt
    13261 gaaagcggga acaggtattt gatcagtctg tcagaccgtt tccggagtgt cacaagtgct
    13321 tcgcgcatcc cttccctggt gctgaactga ggcagaaggg cgttgctcag gccgttggca
    13381 aggagcagga caagagggaa ctcttttgcc ccgtaacggt acatggcaaa gacaccggga
    13441 tcggaataac gggcggagat gatgattccg tcaacatact gtgccgagcc gcttatcagg
    13501 aaggtaagga taagcggcat ccccaccctg aggtgttccc tgatgaatcc gggtgacacc
    13561 ttcaggagtg tatacctcct gagaaggatg attagccaga tccacctcac agcagtgatg
    13621 gcgaatagtc cccagatagc gctcttctca tcgtatccgg caaccaccgg ggccaggacg
    13681 agcagaagct gggcagagta ggtgtagata ccgtactgga gtatcctgtg aggacgcttg
    13741 ttcagcaggt agatgtattc aatgagggca acgggattgc tgagcaggat atagagcagc
    13801 agaaggttgg gatagggaat gtcagggtct gccggtgatg attcgagcac agacctgatg
    13861 ggtatgccca ggatgaacac catcaggctg aagaaagaga tcagaaggaa tgcattgtag
    13921 atctcgggtg attttctacc ctcccttgct ccgtcgtccc tgaaagtgcg gttgcgttca
    13981 tagagggtaa ggagtgactg gattatgccg gtgacccaga agaagctgag caggctggag
    14041 atgaagagaa acagttccca gttgcctatc tgtgcccggg agagatggct cttggtaaat
    14101 acgatgctga taatcaggaa ggtgaggaat ctcagcagct gcacggcctg cagtcccgag
    14161 atgttgtcta tgatcttcag cctgctcaaa ctgtcacgtt atgaatctga aacgcctgga
    14221 tgccgcaata attataccat cttgtttttg gtgcggctaa gttactatat gaatgaattg
    14281 ataacaaaat aaaaataact gtttgcaaaa tgggattttt gctgtttctt tgcactccgt
    14341 aaaacggtgg ttgtagctca gttggttaga gcgccagatt gtggatctgg aggtcgccgg
    14401 ttcgaacccg gtcttccacc ctgtcagaaa cagctgaccc tgatcagggt cagctttatt
    14461 tttttcaaaa atccgagaag cttgcttcta agagttttga aaaataaagc tgagccccgg
    14521 gcttgcccgg ggacagctgt ttctgacaga gtcgcctcct tccggctcga cgtctgaacg
    14581 cagtgaggac ggcaacccgg cattcaccct tttctgacag agtcgcctcc tcccggctcg
    14641 ccgtccgaac cccggcattc agcctttcct gacgacccct tat
//
LOCUS       DAOPXU010000068        14356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_4592_length_14356_cov_15.560241, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000068 DAOPXU010000000
VERSION     DAOPXU010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14356
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4592_length_14356_cov_15.560241"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1192
                     /locus_tag="PKK03_08045"
     CDS             167..1192
                     /locus_tag="PKK03_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNX84390.1"
                     /translation="MNLTEKKFNKFALVREMMETPGIVKSFRPESSIPFIPAFKKKKG
                     LFLTGEGSSRLFPAKRAIFANLRKEFLLPVLTEGCTQALEYNLKDFVVFAASNSGQTK
                     EVIRLVEKLKKKKHDAVFGLTANKNTKLEEFAHAAHVLKCGKEDAVAATKSVVEQALF
                     YDSLLRALRGEKMTGLRKAASQMEEVLTAKIDKKIIAMMHDAEMIWFAGRDNGVAAEL
                     ALKTNEITRKKSGFLEGTFALHGIEEVMNRGDVLIWVDPFEHDRDKFMECIVKGVGVN
                     VIAISDEKTDFPTIVIPKGGDYAEYLQLTAGWNLLVETGISLGIDLDHPVRARKVGNE
                     YVPAAKK"
     gene            1275..2249
                     /locus_tag="PKK03_08050"
     CDS             1275..2249
                     /locus_tag="PKK03_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNX84391.1"
                     /translation="MEKVKTVAGIDIGGTNTTFGFVDRSGKILAEDQIITTHYDEPEV
                     FVAALYEKMMITAQKSGNDFEILGYGIGAPMGNINKGTIEYPADLPWKGIIPLADIFE
                     RHTSLPVIVTNDANAAAVGEMIYGGAREMKNFVVITLGTGLGSGFVVDGKLVYGHDGF
                     AGEIGHTTIRPGTSNRDCGCGRKGCLETYVSATGLKRTVLKIMADRLDNSELRKHSFE
                     ELDSKMIFEAARRGDPIALEAFEHTGAMLGFKLADVVAHTNPEAIFLFGGLALAKELI
                     FEPAKKHMEENLLYIYRDKVKLLPSELSTQNAAVLGASSLVWSTLETA"
     gene            2270..2662
                     /locus_tag="PKK03_08055"
     CDS             2270..2662
                     /locus_tag="PKK03_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84392.1"
                     /translation="MSKYNLPEMMSYIIGALLMVIVFSIAYAYLKPHRLHHARPLSTL
                     ALKGTYLIYLLVMLLVIYFASLRDGGVSQVFDGAEFFVFLVVLFVPTAGIFSRKITRF
                     SGKRVRYNIIFSVINLLMTLTVLVLYRF"
     gene            2721..4373
                     /locus_tag="PKK03_08060"
     CDS             2721..4373
                     /locus_tag="PKK03_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX84393.1"
                     /translation="MIKERFISYVEDSIRKNWEKEALSNYREKGYTYREIAAIMVKQH
                     MAFREYGIREGDTIALLGRNSANWCATYLAVVTYGAVVVPILPDFKPDDLHNIINHSD
                     SKILFADDKLWESLEPEKVKDLQCVMSLDSFEMLLSRSGKITEIYKSLDDKFSQAYPA
                     FSPADIKFSGIDNEKLAVLSYTSGTTGFSKGVMIPHRSLAANIRYAQKNMPLEPGDKV
                     VSFLPLAHTYGCAFEFLFPFTYGCHITILTKTPSPQILIQAFKEIRPRLILSVPLVIE
                     KVYKKQLLPVISKPAMKVMLKVPGINKILFKKVREKLETSFGGNFKELVIGGAAFNAD
                     AEKFFRKIGMRYAVGYGMTECGPLISYDGWRTARLASCGKPVDTLEVKIDSPDPQNVV
                     GEIILRGDNVMLGYYKNEKATKEVIDEDGWMHTGDLGVIDKDGYIYIRGRSKDMLLGP
                     SGKNIYPEEIESVINNYKYVTEAVVISEEDKLVGLIYPDYEALRNDGIGEDGLAAALD
                     TIRKDVNSRLPDYMAVTKFRVHPEEFAKTPKKSIKRYLYMKG"
     gene            4424..5095
                     /locus_tag="PKK03_08065"
     CDS             4424..5095
                     /locus_tag="PKK03_08065"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNX84394.1"
                     /translation="MKKYCIIVAGGSGRRMKSAVPKQFLLLGGRPLLMQTIEKFHTFD
                     SSLEIILVLPEGHHALWEKLCTEHSFNVSHKVVAGGEERFHSVKAGLECVIDDSLVAI
                     HDGVRPLVSHDTIWRCFADAEEFGNAVPFTEPADSVRILAGDDSRPVPRSEVRLIQTP
                     QVFRGSVIIKAYRRGFDPSFTDDATVAEADGVTIHLTHGNRENIKITTPEDLAVAGIL
                     AGTLR"
     gene            5111..6235
                     /locus_tag="PKK03_08070"
     CDS             5111..6235
                     /locus_tag="PKK03_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84395.1"
                     /translation="MEQEPLLDYKKLEVRKVRSADEDITDLIKSTVLGSEGGMRYVML
                     NTPERIRSYGDGLSFLALYKRNSLKGVIGLCRRITSNRGIRYPSAYLRYLAVQSAFQV
                     ERAPGKAGRQLSGAGDSFKQKIFSIFRDPQLAAGSDDEGNAGPNVLYAYVESRNERSR
                     NMINQAGYEYIRSFLTVAFSRFNPEPHPSVEKLSPEEEPAMAKLLEEFYSGYCFYTDE
                     FSFYDHKYYVLRRNGEIVAGVGAIPARYRVVNVPGLWGWMMMKVLHRAPYFRRLFRPE
                     EFRYIILNAIYCREGSEDLLPDLFDAVCAEEGYHTALTWLDDHSHLYETLRTNRRMGA
                     LNRLLNAKPGLVYALFSGLTPGETEKFYDSPAYISGFDFS"
     gene            6380..7708
                     /locus_tag="PKK03_08075"
     CDS             6380..7708
                     /locus_tag="PKK03_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNX84396.1"
                     /translation="MSVIHSLSARLLLAAALFLALAGQASAQPYIPDRTYDDERMKWF
                     ADAKLGIFIHWGIYAVNGIDESWAFYNELIGYDDYMKQLDGFTASSYDPDAWVRLIRE
                     SGARYAVLTSKHHDGVALWDTKLSRLNTVDSTPAGRDLVKPFVKELRRTGLKVGLYYS
                     LLDWSHPDYPNLTKTVKRYTDDQERWQRFMQFNLGQIAELQKEFKPDLWWFDGDWEQS
                     AEKWGAEGIRKTILERNPSAIINSRLAGYGDYGTPEQGLPSDRPADKYWELCMTINDS
                     WGYQGNDRNYKSPSQVVRIFAGCIGMGGNMLLDIGPKADGTIPEEQVEVLKEMGRWTS
                     KHKQAIYGTVAGLPDGLFAGPSTMSRDSTMLYLFFPGDGGGELMLEGVKNRINKAFVI
                     GNGTNLEVKEYLRPWWSDHAGVYFIRVPEETLDKTMTVVGISLEGKLLTE"
     gene            7720..9192
                     /locus_tag="PKK03_08080"
     CDS             7720..9192
                     /locus_tag="PKK03_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNX84397.1"
                     /translation="MKSIFRSAGKLMQAYLPALVITALFTSPLMAQDHDDRYVPETDP
                     LVLQKLEEWQDMKFGLLMHWGPYSQWGIVESWSICAEDEDWCRRKNPDYTEYKKQYEA
                     LKLSFNPIAFNPEKWAAAARNAGMKYVVFTTKHHDGFSMFDTKLTDYRITDPECPFSV
                     NPRANVTKEIFKAFREQGLWTGAYFSKPDWHNEYYWWPNFATPDRHVNYSIPDYPERW
                     EKFVDFTHGQIMELLGGDYGRIDILWLDGGWVRKMTDEEVLKYRMAQGNKMTRVQNQD
                     IRMDELVAKARQAQPGLIVVDRAVYGKNQNYLTPENRVPGRALPYPWESCIIAGGGWA
                     WVPDPKFMSPREAVHLLVDIVVKGGNLLLNIGPAPDGTWPEEAYTLLEEIGGWMKVNG
                     EAIYGTRALTPHKDGKACITRKGNDTWYLYYMADEGENVPASVTMNGLTLPAGAKVSA
                     LGSGAICRWKNGNGSFTVSVPQQVRNKPSSDYAWVFRIDL"
     gene            complement(9418..10182)
                     /locus_tag="PKK03_08085"
     CDS             complement(9418..10182)
                     /locus_tag="PKK03_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNX84398.1"
                     /translation="MNSQKIRSMIVEDEPEALDLLAGLLEATGEVTVVASTTNPFEAI
                     DLLLLHTPDILFLDIKMPGMSGFDIINEMNRLTALTPRVVFTTAHDEYAIKAFDCAAF
                     DYLLKPIDPDRLAETLRRHSSQANGGKNDQLRQQSFLNNEKILIFRGVTGVTFIDTDD
                     VVYITADGNYSSFHFISGRVETVTALIGNIEAQLGKQYFRTGRSCIVNTSYLARIDMK
                     QMSCVFIRGEKEYRCEISRDKVKMLMDFMKNRFSEF"
     gene            complement(10207..13290)
                     /locus_tag="PKK03_08090"
     CDS             complement(10207..13290)
                     /locus_tag="PKK03_08090"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HNX84399.1"
                     /translation="MKLDKRVRGVMRPAAVLLLAPARYPALLPAPARLSALTQTAARY
                     PALLPAPARLSALTQTAARYPALLPAPARLSALTQTVARLPALLLVILLATTGLSLRA
                     QTYSYREYTSDDGLPQTQSTSVVQDSRGYLWIPTRNGLARFDGYTFNSYLRKDGLPSN
                     LITSVIEDRGGIIWAITANGVARFNGANFESYPIPDSFRVKQLDRVAIGRDTNSFFLS
                     ALIDNDNQIILLFENGRYTDFASRHPLLRGTSFSVAAANQRDSLLYLIDKNNEAFAYE
                     KGQLKPLNKSDVYEIDIVDGQVRYRYMSDALFPTIEPFYWESGNLAMYYEDREGTIWA
                     GTETSIYRLMSQAFVTYDRENGLPEETWAVVADPLGGIWTGSVEGELRYFDGEKFRGK
                     NEYQALFGHPVAFFRGSTTLSNGEIWFSTVEGVLIWDGRRFRLPDLSPDPYQVCIIYE
                     DPVDKSIFVGTDRGLFHIRDGRVTIHEEMSWYGYGIVESVARDHEGHYWLAGHYGVVY
                     FDGKNFIPFRSSPAPAEMVWGVVCDHMGNIWSAGSDGLYICDPNEPVFDRALPEEVNL
                     PASVIRDLGNHRLIIGRMMDICIIDLDKYYSGQPDYYSITGRSQGFPGNDCQDNGIIK
                     DKEGRWWILTSDKLIRFDPDKISRNTYPPMNHITKVELQDEETPWKTALEASLFYDTT
                     SFLRVRGRQNRIRISYTGISTRNPEGVRYRYRMAGFNDNWSEKTAERSVVYTNLPPGE
                     YTFEVQAINADGVLSEDPDKLEVSVVPTFIQSKVAIALMALIALGLIVFLSFQIRKAV
                     LERRVITARIQAEAYRLQLNSVIKQFDPHFTFNAVTSVGSLIMKGEKEKAYNYFIKLS
                     NLLRSIIADSNALLKPLEEELEFVTRYCELQKLRFGNRFEYRINVAPDVNLKSPVPKM
                     IIQSFAENAIKHGLENKKERGILEISIMNLEGGVEVKIRDNGIGRAAAAKMRTHGTGT
                     GLKNISGIVDTLNKVNREKITFNLTDLYDNGKASGTEVTVFLPYNYSLNLPADLL"
     gene            complement(13575..14000)
                     /locus_tag="PKK03_08095"
     CDS             complement(13575..14000)
                     /locus_tag="PKK03_08095"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNX84400.1"
                     /translation="MKKVALISGLLFMVVYFWVNTFSKEESSFDVVYPSVSVNSFTDY
                     ENVATPVTGNTVSPPADNNQVKYNYLIVASFTDIDQAGRMAEEYAGKLQADMFVLPAT
                     TNGYYRVSYGRYSTTAEALSALETLKGNNFPDAWLLASK"
     gene            14211..14336
                     /locus_tag="PKK03_08100"
     CDS             14211..14336
                     /locus_tag="PKK03_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84401.1"
                     /translation="MPAPASSLWGVLEFRLLADKRTSDLFRQLTDPPVGGLNQLS"
BASE COUNT         3494 a         3555 c         3967 g         3340 t
ORIGIN      
        1 gatgatttct tcggctatgt actggagggt aacaaaaact acggtaaatt agcgggtgta
       61 gaatatgccg agccgtactg gcagcacctg ggcggcggat tccagaaaga cccgcaggtg
      121 cagcgcgacc tgaaagaata cattgtataa caataactta actgacatga acctgaccga
      181 gaagaagttc aataagtttg cgctggtgcg cgaaatgatg gaaactcccg gaatagtcaa
      241 atcattccgt cccgaatctt ccattccttt cattcctgcc tttaaaaaga agaaaggtct
      301 cttcctgacg ggagagggaa gcagcaggct cttccccgcg aagagggcca tctttgccaa
      361 cctcaggaag gagttcctcc tgccggtcct caccgaagga tgcacccagg cccttgagta
      421 caacctgaag gacttcgtgg tctttgcggc ttccaactcg ggtcagacca aggaagtcat
      481 caggctcgtc gagaagctga aaaagaaaaa gcatgacgcc gttttcggcc ttacggccaa
      541 caaaaacacc aaactggagg agttcgccca tgcggctcat gtgcttaagt gcggcaagga
      601 ggatgccgta gccgccacca agtcagtggt tgagcaggct ctcttctatg actcgctgct
      661 gcgcgccctc aggggagaga agatgaccgg actgcgcaag gctgcatcgc agatggagga
      721 ggtgctgaca gcgaaaattg acaaaaagat cattgccatg atgcatgatg cggagatgat
      781 ctggttcgcg ggacgcgata acggtgtggc cgccgaactg gctctcaaaa ccaacgagat
      841 cacccgcaag aaatcaggtt tcctcgaggg aactttcgcc ctgcatggca tcgaggaggt
      901 gatgaaccgg ggtgatgtgc tcatctgggt cgaccccttc gaacatgacc gcgacaagtt
      961 catggagtgc atcgtcaagg gtgtgggggt gaatgtcatt gccatctcgg atgagaagac
     1021 cgacttcccg accatcgtca tcccgaaggg aggcgactat gccgagtacc tgcagctgac
     1081 tgccgggtgg aacctgctgg ttgagaccgg catctccctc ggcattgacc ttgaccatcc
     1141 cgtgcgcgca cggaaggttg gcaatgagta cgttccggca gctaaaaagt agaatcctgc
     1201 tgccaggtta cagttttttt tcgcagctat gatctaaagc gtatatttac gttaagcaaa
     1261 ctctctttct gaatatggaa aaggtaaaaa ctgttgcagg gattgatatc ggaggcacca
     1321 acaccacttt cgggtttgtt gaccggtcag gaaagatcct ggccgaggac cagatcatca
     1381 caacccatta tgatgagcct gaggtttttg ttgctgcgct ctatgagaag atgatgatca
     1441 ccgcgcagaa atccggaaat gattttgaga ttctcgggta tggaataggg gcaccaatgg
     1501 gtaacatcaa caagggaacc attgagtacc ctgctgacct gccatggaag gggatcatcc
     1561 cgctggctga tattttcgag cgccacacct cgctgccggt catcgtcacc aacgatgcca
     1621 acgcggcagc cgtgggcgag atgatctacg gcggcgccag ggagatgaag aacttcgtgg
     1681 tgataaccct cggcaccggc ctcggcagcg gcttcgtcgt tgacggcaag ctggtatacg
     1741 gccatgacgg cttcgcgggc gagataggac atacaaccat taggcctggg acgtcaaacc
     1801 gcgactgcgg atgcggacgg aaggggtgcc tcgagactta tgtctcagca acgggcctga
     1861 aaaggacggt gctgaagata atggcagaca ggcttgacaa cagcgaactg cggaagcaca
     1921 gcttcgaaga gcttgattcg aagatgatct ttgaggctgc gcgcaggggt gacccgatag
     1981 ccctcgaggc gtttgaacac acaggagcca tgctcgggtt caagctggcc gatgtggtgg
     2041 ctcacaccaa tcccgaggct atcttcctct tcgggggcct cgccctcgcc aaggagctga
     2101 tctttgaacc ggccaaaaag catatggagg aaaaccttct ctatatctac cgtgacaagg
     2161 tgaaattgct gccctcggag ctgagcaccc agaatgcagc ggttcttggc gcgagctcac
     2221 tggtatggtc tacgctggag acagcatgaa tcctgacttc gggaactgaa tgagcaaata
     2281 taacctgccg gagatgatga gctatatcat tggagcgctc ctgatggtga tagtattttc
     2341 cattgcctat gcttatctca agccgcacag gctgcatcat gccaggcctc tctcaacgct
     2401 ggctctgaaa ggcacatacc tgatctacct gctggtgatg ctgctggtga tctatttcgc
     2461 cagcctcagg gacggcgggg tgagccaggt ctttgacggg gcagagttct tcgtgttcct
     2521 ggtggtgctc ttcgtgccaa cggccggcat cttttcaaga aaaataaccc gcttcagcgg
     2581 aaagagagtc aggtacaaca taatcttcag tgtgatcaac ctgttaatga cactgacagt
     2641 gcttgtgctg tacaggttct gataacagat attttttaaa agcttacatt tgcccaatga
     2701 aataaaaaaa ctccaaaatc atgatcaagg aacgattcat ctcctatgtg gaggatagta
     2761 tcagaaagaa ctgggaaaaa gaggcgcttt cgaattaccg ggagaaaggt tacacgtaca
     2821 gggagatcgc cgcgataatg gtgaaacagc acatggcttt cagggagtac ggcatccgcg
     2881 aaggtgacac tatcgccctg ctcgggcgaa actctgccaa ctggtgtgcc acgtacctgg
     2941 cagtagttac ctatggcgca gtggtggtgc cgattctgcc tgacttcaaa cctgacgatc
     3001 ttcacaatat catcaaccac tccgactcaa agatcctctt tgctgacgac aagctgtggg
     3061 aatctcttga acccgaaaag gtaaaggatc ttcagtgcgt aatgagcctt gactcgtttg
     3121 agatgctcct gagccgttct gggaagatca cggagatcta caaatctctt gatgacaagt
     3181 tctcacaggc atacccggca ttcagtccgg ctgacattaa attttccggc atagacaatg
     3241 agaaactggc agtgctgagc tatacttcgg gtacaacagg tttctccaaa ggggtgatga
     3301 tacctcacag gagcctcgct gcaaacatcc gatacgcaca gaagaacatg ccgctggaac
     3361 ccggtgacaa ggttgtttcc ttccttccgc tggcacacac atatggctgc gccttcgagt
     3421 ttctcttccc cttcacctac ggatgccaca tcaccattct gaccaagacc ccgtcgccgc
     3481 agatactcat ccaggctttc aaggagatca ggccgaggct gatactctcg gtcccgctgg
     3541 tcatcgagaa ggtgtacaag aagcagctgc ttccggttat cagcaagcct gccatgaagg
     3601 tgatgcttaa ggtcccggga attaacaaaa ttctattcaa aaaggtgcgc gagaagcttg
     3661 agacgagctt tggcggcaat ttcaaggagc tggttatcgg gggtgctgca ttcaacgctg
     3721 acgccgaaaa gttctttagg aagataggga tgcgctatgc cgtgggttac ggtatgaccg
     3781 agtgcgggcc gctgatcagt tatgacggct ggagaacggc acgcctggca tcatgcggaa
     3841 aacccgttga caccctggaa gtaaagattg actcccccga tcctcagaat gtggtgggcg
     3901 agatcatact ccgcggtgac aacgtgatgc tcggatatta caagaacgag aaggccacga
     3961 aggaggtcat tgatgaggac ggctggatgc ataccggaga cctcggcgtg attgacaagg
     4021 acggttacat ctatatcagg ggccgcagca aggacatgct cctcggccca tcgggcaaga
     4081 atatctaccc cgaagagatc gagtctgtca taaacaacta taagtatgtg accgaagcgg
     4141 ttgtcatctc tgaggaggac aagcttgtcg ggctcatcta tcctgattat gaggcgctgc
     4201 gcaatgacgg catcggagaa gacggacttg cggctgccct tgacacaatc aggaaggatg
     4261 tcaacagcag gcttcctgac tatatggccg tcacaaagtt cagggtgcat cccgaagagt
     4321 ttgccaagac gccgaagaag agcatcaagc gctatcttta catgaaaggt taatataaca
     4381 gagcgaaggg gccccgaaag ggcctctccc atattatcgg gaagtgaaga agtactgtat
     4441 catagttgcc ggcggaagcg gacggagaat gaaatcagct gtcccgaagc agttcctcct
     4501 gctgggaggg aggccgctgc tgatgcagac cattgagaaa ttccacacgt ttgacagttc
     4561 cctggagatc atactggttc ttccggaggg ccatcatgcg ttatgggaaa agctctgtac
     4621 ggagcactct tttaacgtca gtcacaaggt tgtggcaggc ggggaggagc ggttccattc
     4681 cgtgaaggca gggttggagt gcgtcattga cgattccctt gtcgccatcc atgacggcgt
     4741 caggccgctg gtgagccatg acaccatatg gagatgcttc gccgatgctg aagagttcgg
     4801 caatgccgtg ccctttaccg aaccggcaga ctccgtaaga atcctggcag gtgatgacag
     4861 caggccggtg ccgaggagtg aagtgaggct gatccagaca ccacaggtgt ttcgcggcag
     4921 cgtgatcatc aaagcctacc gccgcggatt tgacccctca tttactgacg acgccaccgt
     4981 tgcagaagct gacggggtca ccatacacct tacccacgga aacagggaga acatcaagat
     5041 aacaacgccg gaggacctgg cagtggccgg gatactggcc ggaacactca ggtaatgaaa
     5101 cagataaaag atggagcagg agccgttgct cgattataag aaacttgaag tcagaaaagt
     5161 caggagcgct gacgaggata taactgacct gataaagtca acagtccttg gttcggaggg
     5221 cggcatgcga tacgtcatgc tgaacacgcc ggaacggatc aggtcatacg gtgacgggct
     5281 ttctttcctt gcactgtaca agagaaacag cctcaagggg gtgataggac tctgccggcg
     5341 gattacatca aaccgtggca tccggtatcc ctctgcttat ctcaggtatc ttgcggtgca
     5401 gagtgcgttc caggttgaaa gggcgccagg gaaagccggg agacagctgt ccggggccgg
     5461 agacagcttc aagcagaaga tattttccat tttcagggac ccgcagctgg cagccggatc
     5521 tgatgatgaa ggcaacgccg gcccgaatgt gctttatgcc tatgtggaga gccgcaatga
     5581 gcgatcgagg aacatgatca accaggcggg ctatgaatat atccgctcgt tcctgacagt
     5641 ggccttcagc aggttcaatc cggagcctca tccttcggtg gagaagctct ccccggagga
     5701 ggagcctgcc atggcaaagc tcctggaaga gttttacagc ggctactgtt tttataccga
     5761 tgagttttcg ttttatgacc ataaatatta tgtgcttcgc cggaacggcg agatagtggc
     5821 aggcgtggga gccataccgg cacgttacag ggtggtaaac gtaccgggct tatggggctg
     5881 gatgatgatg aaggtgctgc accgtgctcc gtatttcagg agactgttcc ggccggagga
     5941 attcaggtac atcatcctga atgccattta ttgccgcgaa ggcagcgagg atctgctgcc
     6001 ggatcttttt gatgccgtct gtgcagagga gggttatcat actgcgctga catggcttga
     6061 tgatcactca cacctctatg agacccttcg caccaacagg aggatgggcg ctctcaacag
     6121 gctgctgaac gcgaagccgg gactggtgta tgccctcttt tcggggctca ctcccgggga
     6181 gaccgagaag ttctatgact ctccggcata catctcggga tttgactttt catagacacc
     6241 tgccccgggc tgttgtgtgc ccggagggac tgatcccggg tggcggagag cctgcagaga
     6301 ctgatcccgg gtggcggaga gcctgcagag actgatcccg ggcagtgata ttttgcggaa
     6361 atttgtaaaa caatatacta tgagcgtaat tcattctctg tcagcacggt tgctgctggc
     6421 cgcagctctg ttcctggcac tcgccgggca ggcttcagcc cagccctata taccggaccg
     6481 gacgtacgat gatgagcgga tgaagtggtt cgcggatgcg aaactcggaa tattcatcca
     6541 ctggggaatc tatgccgtca acgggattga cgagtcgtgg gcattctaca acgaactcat
     6601 cggctatgat gattacatga agcagcttga cggcttcacc gcctcgtcat atgacccgga
     6661 cgcatgggtc aggctgatca gggagagcgg cgcccgctat gccgtgctta cctcaaaaca
     6721 tcatgacggg gtggccctct gggacacgaa gctcagccgg ctcaatacgg ttgacagcac
     6781 cccggcaggc cgtgacctgg tgaaaccatt cgtgaaagag ctgcggcgca cgggattgaa
     6841 agtgggactt tactattcgt tgctcgactg gtcgcatccc gattacccga accttaccaa
     6901 aacagttaaa cggtataccg atgaccagga gcgatggcag cggttcatgc agttcaatct
     6961 cgggcagatt gccgagctgc agaaggagtt caaacctgac ctgtggtggt ttgacggcga
     7021 ctgggaacag agtgccgaaa aatggggcgc agagggtata aggaaaacga tcctggaaag
     7081 gaatccttcc gccattatca attcccggct ggcaggatac ggtgattatg gcactcccga
     7141 acagggactg ccctcagacc gtccggccga caagtactgg gaactctgta tgacaataaa
     7201 tgattcctgg ggataccagg ggaatgacag gaactacaag agcccgtcgc aggtggtgcg
     7261 gatctttgcc ggatgcatcg ggatgggagg aaacatgctc cttgacattg gcccgaaggc
     7321 cgacggaacc atcccggaag aacaggttga ggtgcttaag gagatgggac gctggacctc
     7381 caaacacaaa caggcaattt acggaactgt ggccggactg cccgacggcc ttttcgcggg
     7441 gccctccacc atgagccgtg acagcaccat gctatatctt ttctttcccg gtgacggcgg
     7501 cggagagctg atgctcgagg gggtgaagaa ccgcatcaac aaggctttcg ttataggcaa
     7561 cggcaccaac ctcgaggtga aggaatacct gagaccatgg tggagcgacc atgccggggt
     7621 atacttcatc agggtgcccg aagagacact ggataagaca atgaccgtgg tgggcatctc
     7681 actcgaaggc aaacttttaa ctgaataata tctgttggca tgaaaagtat cttccgatca
     7741 gcagggaaat tgatgcaggc gtatctgccg gcattggtga ttacagccct ttttacctca
     7801 ccgctcatgg cgcaggatca tgatgataga tatgtcccgg agactgatcc gctggtgctt
     7861 cagaagctcg aagagtggca ggacatgaag ttcggcctcc tgatgcactg gggaccatac
     7921 agccagtggg ggatagttga atcatggtcg atctgcgccg aggatgaaga ctggtgccgc
     7981 cgtaaaaacc ctgattacac agagtataaa aagcagtatg aagccctgaa gctcagtttt
     8041 aatccgattg ctttcaatcc ggagaaatgg gccgctgcag cacgcaacgc ggggatgaaa
     8101 tacgtggtct tcaccaccaa gcatcatgac ggcttttcaa tgtttgacac aaaactgaca
     8161 gactatagga taactgaccc tgagtgcccg ttcagcgtca atccccgcgc aaacgtaacg
     8221 aaggagatat ttaaggcttt ccgcgaacag ggattatgga ccggggccta cttctcgaaa
     8281 cccgactggc acaacgagta ctactggtgg ccaaacttcg ccactccgga ccgccatgtc
     8341 aactacagca ttccggatta cccggagcgc tgggagaagt ttgtggattt cacccacggg
     8401 cagatcatgg aactcctcgg cggcgattac ggcaggattg acatcctgtg gctcgacggc
     8461 ggctgggtca ggaagatgac agatgaggag gtgcttaaat acaggatggc ccagggcaac
     8521 aagatgacca gggtgcagaa ccaggatatc cgtatggatg agctggtggc taaagcacgg
     8581 caggcacagc cgggactgat tgttgttgac agggccgtgt acggaaagaa ccagaattac
     8641 ctgacacctg agaacagggt gcccgggagg gcgctgccat acccgtggga gtcgtgcatc
     8701 attgccggag gaggctgggc atgggtgcct gatccgaagt tcatgtcgcc ccgcgaagct
     8761 gttcatctgc tggttgatat cgttgtcaag gggggaaacc tgttgctgaa cataggtccg
     8821 gctcctgatg gcacgtggcc tgaagaagct tatacgcttc ttgaggagat agggggatgg
     8881 atgaaggtca acggtgaggc catttacggc acaagggcgc ttaccccgca taaagacggc
     8941 aaggcatgta tcacccggaa gggcaatgac acctggtatc tttattatat ggctgatgaa
     9001 ggtgaaaacg taccggcatc cgttacgatg aacggactca ccctgccggc cggggcaaag
     9061 gtatcagcgc ttggttccgg agcaatatgc aggtggaaga acgggaacgg gtcatttact
     9121 gtttctgtgc cacagcaggt cagaaacaaa ccctcgtctg attacgcatg ggttttccgg
     9181 attgacttgt aattcaggta tcgccagaaa acgcgccaca cagaccattc tgctgtgatc
     9241 tgtgtggcct gacagggaaa tggggggaag aaccacctgt cgtatattga aatacagcgg
     9301 taaagattga ataaaattgc aatcttccaa gtcaaaatgt cctctattgt atgaaaggca
     9361 gcttctaaag tatggacaat cccccttgct aaatgtgtga aaaaatgtta atttatttta
     9421 gaattcagaa aaacggtttt tcatgaaatc catgagcatt tttaccttgt cacgcgagat
     9481 ctcgcacctg tattcctttt ctcctctgat gaatacgcag gacatctgtt tcatgtcgat
     9541 gcgtgcaaga tatgacgtgt tgacgatgca tgacctgccg gtcctgaaat actgcttccc
     9601 caactgcgcc tcaatgttcc cgatgagtgc ggttactgtt tcgacccttc ctgatatgaa
     9661 gtgaaagctt gagtagtttc cgtcagcggt aatataaacc acgtcatcgg tatcaatgaa
     9721 agtcaccccg gtgacaccgc ggaaaatcag tatcttttcg ttattcagga aagactgttg
     9781 ccgcaactgg tcattcttcc cgccattggc ctggctgctg tggcggcgga gagtctctgc
     9841 cagccggtca gggtcaattg gtttcagaag gtaatcgaag gcagcgcagt cgaacgcttt
     9901 gattgcatac tcatcatggg cagttgtgaa aacaacgcgc ggtgtcaggg cggtcagcct
     9961 gttcatctca ttgattatgt caaatcctga catccccggc attttaatat cgaggaacag
    10021 gatgtcagga gtatgtaaca gcaaaagatc gatggcctca aagggatttg tcgttgaggc
    10081 aaccaccgta acctctcctg tggcttccag cagtcctgcc agaaggtcga gggcctcagg
    10141 ttcgtcttcg acgatcattg atctgatttt ctgggaattc atgttatccg ggtttgggtt
    10201 ggtttatcac aacagatcag caggcaggtt gagggagtaa ttgtacggaa ggaatacggt
    10261 tacctcggtg ccagatgcct tgccgttgtc atacaggtca gtcaggttga atgtgatttt
    10321 ttccctgttc accttattga gagtatccac aatacctgaa atattcttca gtcccgtgcc
    10381 ggtgccgtgg gtacgcattt tcgcagcagc agcccttcct attccgttgt cacggatttt
    10441 cacttccacc cctccttcga ggttcatgat gcttatctca aggatccccc gctccttctt
    10501 gttttcaagg ccatgtttta tcgcgttttc agcgaacgac tgtatgatca tctttggtac
    10561 gggactcttc aggtttacgt caggtgcaac atttatcctg tattcgaacc tgttgccaaa
    10621 gcgcagtttt tgtagttcac agtagcgtgt aacgaactca agctcctcct ccagcggctt
    10681 caggagggcg ttgctgtcag cgataatgga acgaagcagg tttgacagtt ttatgaaata
    10741 gttataggct ttttcctttt cgcctttcat tataagtgac ccgacggagg taacggcatt
    10801 gaacgtgaaa tgcgggtcaa actgttttat caccgagttt agctgcagcc tgtatgcctc
    10861 agcctgaatg cgggcagtta taaccctcct ttccagtaca gcttttcgta tctggaatga
    10921 gaggaacact atcagcccga gggctataag agccatcagt gcaatagcga cttttgactg
    10981 gatgaatgtt ggcacaacgc tgacctccag tttatcgggg tcttcagaca gaactccgtc
    11041 ggcgtttatc gcctggactt cgaaagtata ttcgccggga ggcagattgg tgtaaacaac
    11101 tgatcgctct gcggtctttt ctgaccagtt gtcattaaag cccgccattc tgtaacgata
    11161 cctgacaccc tccggatttc ttgtcgagat accggtgtag ctgattctta tacggttctg
    11221 tcttcccctg accctcagga agctggtggt gtcatagaac agggaggcct ccagcgccgt
    11281 tttccagggt gtttcttcgt cctggagctc gactttggta atgtggttca tgggaggata
    11341 tgtgttcctg ctgatcttat ccggatcaaa acggatcagc ttgtcagagg tcagaatcca
    11401 ccatctgcct tccttatcct ttataattcc gttgtcctgg cagtcattgc ccgggaatcc
    11461 ctgactcctg cctgttatgg aataataatc gggctgccct gaataatatt tgtcaagatc
    11521 gatgatgcag atatccatca ttcgtccgat gatcagcctg tggtttccca ggtcacggat
    11581 gacactggca ggaaggttga cctcttcggg cagggccctg tcaaagacag gctcgttcgg
    11641 atcgcatatg tacaggccgt cagagcccgc actccagatg tttcccatgt ggtcgcaaac
    11701 cactccccat accatctcgg ccggggctgg cgatgaacgg aacgggatga aatttttccc
    11761 gtcaaaataa accacaccgt agtgtccggc cagccagtaa tggccctcgt gatccctggc
    11821 gacactctcc actattccgt atccatacca ggacatctcc tcatggatgg ttaccctgcc
    11881 atccctgatg tggaacaggc cccggtcagt cccgacaaat atgcttttat cgacgggatc
    11941 ctcgtagatg atacagacct ggtacggatc gggagacaga tctggcaacc tgaatcttct
    12001 gccatcccag atcagcacac cctctactgt tgaaaaccat atctcgccgt tgctcagggt
    12061 ggtgctcccc ctgaagaagg ccaccgggtg gccgaacagc gcctggtatt cattttttcc
    12121 ccggaatttt tccccgtcaa agtatctcag ttcaccttcg acagaaccgg tccagattcc
    12181 tccgagagga tcggccacca cagcccaggt ttcctccggt aacccgtttt ccctgtcata
    12241 tgttacgaat gcctgtgaca taagcctgta gatgctggtt tccgtgccag cccagattgt
    12301 cccctcccgg tcctcatagt acatcgcgag gttaccgctt tcccaataga atggttctat
    12361 ggtcgggaag agggcatctg acatgtaccg gtaccggacc tggccatcga ctatgtcaat
    12421 ttcgtagaca tcacttttgt tgagtggttt cagctggccc ttttcatagg cgaaggcttc
    12481 attatttttg tctatcaggt agaggagtga gtcgcgctga ttggccgctg cgactgaaaa
    12541 actggtgccc cggagcagcg gatggcggga ggcgaaatcg gtgtacctgc cgttttcaaa
    12601 aaggagaatt atctggttgt cattgtcgat taaagctgac aggaagaatg aattggtgtc
    12661 acgtccgatt gccaccctgt cgagctgttt gaccctgaat gaatcaggga tgggataact
    12721 ttcgaagttt gccccgttga agcgagccac cccgttcgcg gtgatggccc atattatgcc
    12781 ccccctgtct tcaatcaccg aggttataag gtttgacggc aggccgtcct tgcgcaggta
    12841 ggaattgaag gtataaccgt cgaatcttgc caggccgttc ctggtgggta tccagaggta
    12901 tcccctgctg tcctgcacca cggatgtcga ctgtgtctgt ggaaggccgt cgtcggaggt
    12961 atattcccgg taggagtaag tctgggcacg gagggagagg cctgttgttg caagcaaaat
    13021 gacaagcagc agggcaggga gccgtgcaac tgtttgcgtc agggcagaga gccttgcagg
    13081 cgctggcaac agggcaggat accgtgcagc tgtttgcgtc agggcagaga gccttgcagg
    13141 tgctggcaac agggcaggat accgtgcagc tgtttgcgtc agggcagaga gccttgcagg
    13201 tgctggcaac agggcaggat accgtgcagg cgccagcaac agtacagccg ccggccgcat
    13261 aacccctcgt actctcttgt ccagcttcat tatcagtgtt ctgcatgtaa atatatctat
    13321 ttagtcagga atataaaatg agggtgggca aacaatcgaa gggcattgga attacgattt
    13381 gagatttgag atttgcgatt tgaggctttt ggccgtaggc catgcagcat tgtagaactg
    13441 gtcattgatg gatggcgatg gtcgtagacc atcaggcatt gtaacaggca tagctacccc
    13501 ctttggccgt aggccaaacc agagtgcccc gatggggctg ataactttct gattatgctt
    13561 aatgtgagtg caggctactt cgaggccagc agccaggcat ccggaaaatt gttgcccttc
    13621 agggtttcga gcgctgaaag tgcttccgcg gtcgtggaat accttccgta gctgaccctg
    13681 taatatccat ttgtggttgc gggaaggaca aacatatcag cctgaagctt accggcatac
    13741 tcctcggcca ttctgccagc ctgatcaatg tcggtgaagc ttgctacgat caggtagttg
    13801 tattttacct ggttattgtc agccggcggc gaaacagtgt tgcctgtcac cggagtggcg
    13861 acattctcat aatccgtaaa tgagttgact gatactgagg gataaacaac gtcaaatgaa
    13921 gactcctctt tggagaatgt gttcacccag aagtaaacta ccatgaacag gaggccgcta
    13981 atcaaggcaa ctttcttcat ctggtaatca ttccttgttt ataatatata accagattta
    14041 tatgaataat gttccgcttc tacttgctaa tccaaattta cgccatgaaa taagcgaaat
    14101 caagtttttt taaccgcagt tctcaacaat gaatagaagt ttcaaccggt cagggctgtt
    14161 tttggcagac ggtgcggggg agggtgtggc attgtaaaag agccggcatc atgccagctc
    14221 ctgcgtcttc actgtggggc gtactggaat tccgcctgct ggcggacaag cgaaccagtg
    14281 acctcttccg tcagctgacg gatccgccgg ttggcggact aaaccaactg agctaacgtc
    14341 ccattcagtg caagtt
//
LOCUS       DAOPXU010000069        14325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_4611_length_14325_cov_11.399720, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000069 DAOPXU010000000
VERSION     DAOPXU010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14325
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4611_length_14325_cov_11.399720"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="PKK03_08105"
     CDS             <1..1206
                     /locus_tag="PKK03_08105"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNX84402.1"
                     /translation="EKNNEFTLLGQGSYELKIGSDEVYTIKIWKEGFEPFVKSYTRTN
                     NGTLTENVELQNRVVKVVASPNTTEVYADNVYVGKGGEELEVVVRYNNMVNLEVRGEG
                     YQTVRRSYYNMENTEIPPLKENIELAERLVKILASPPGCLISVDGKPVGETSAEVVVP
                     KGSCITLKVVKDGYAPFEKSYCNKPEVALPPISEEIILKDRIVQINTTPEDAAIRVDG
                     RAVGKGNYSLLVKRDQCAEIEVMKDGFDTNKKSYCNKANMSEPPVTDHIRLAEDEAWL
                     SSIRSDQANVNFSIPVNSSLGNDAAWKIIGQIVMEKFDVIEISDPVTGYLRTAWNVKL
                     FGNGKTIRTRCIVKLGNSNPLRYVIKIVSEESIVPGTSVKEDENFKEWDRILNTYKDI
                     IGEIQARIS"
     gene            complement(1240..1755)
                     /locus_tag="PKK03_08110"
     CDS             complement(1240..1755)
                     /locus_tag="PKK03_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84403.1"
                     /translation="MIRSVIIAVICTISCMSAAAQQPGPITTEKKGLAIQYFQNGQRL
                     DRKEIRTVLSGYTGSADELKKSSRNSGIGLGLLGGGCLVIGASSLISTLNDLETLNSG
                     SLDVSGANTGPFLIGCGMALAGIPFLLKGNAQFVRSINLYNSHSSAGIASGPSLTIAI
                     TPVTAGIKIIF"
     gene            2000..2443
                     /locus_tag="PKK03_08115"
     CDS             2000..2443
                     /locus_tag="PKK03_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84404.1"
                     /translation="MRTKNYLIGLVIGVLIFSVSTLETNAQSQYAAWRDRSDELPGMI
                     SDGEMLLIAGGAVLVIGGVITYIIIKKKQDNKLEAMLPDCCGMTGTERIVSGRTQGSS
                     LGSLYNDISRATDQASVLIFSRLDKIPNPGQGQNNVLSMGVLIKF"
     gene            complement(2536..3510)
                     /locus_tag="PKK03_08120"
     CDS             complement(2536..3510)
                     /locus_tag="PKK03_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84405.1"
                     /translation="MKMKLLCSIACVLAFSAFFASGLNAQDRNEVIAVYNEGAKAIQT
                     DVPTAITAFEKVIELSDKVGETTNDLKQKAVQVLPGLYYKLAATAYNEKKPAAEIIAA
                     SKLAGAMAEKYGSKTHGENSKKILVNGYYKMATEFFSADDYDNAMLAFDSVLTLNPDH
                     IASIYNKALIYKNRDEADLFEQTIDQFLAKLDPAKDADKAVQAKGAALEYFRAAGSKA
                     NSANKLDEALALLNRAAKYGDDKTMFYFFADVYNKKNSFTEGAEFAQKGLDLETGDAE
                     AKAMFYYQLAVAQVGKGDTAAACESFKNALYGPFAEAAKAQRTNLKCQ"
     gene            3747..4949
                     /locus_tag="PKK03_08125"
     CDS             3747..4949
                     /locus_tag="PKK03_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84406.1"
                     /translation="MPESFGKKPEKGNVVRVAWAAHRGAPASTAAEAEMQIRHFLAPD
                     DFIITGTRPDIILFMSGGSERRAIELADQGKPVLLLSICGNNAYASATEVMAWMVNNN
                     RMAILSDLSEAAESGLLNRWCRLAGAWERLDGARAGLIGAVSEWLVASGVSAQTLLSR
                     FGISLTEIPWSGLPDYTSSQPDQALMNRFGGHSPEGLDEAARVLTLLRKVIDENRLDA
                     VGVECFSLVQERKVTACLALAQLNTEGTVAACEGDLASMAGMMAGKALTGRVPWMANT
                     TRLTGKSVILSHCTAPFDLVSDVQLLSHYETRLSLAVDGKIRETEVTLFRFSDMLDRA
                     FVADGRIISHPRIANACRTQVETEIDKKALQLLKEKPLGNHLLLLPGNHSDFLRLACR
                     YKGIETIS"
     gene            5006..5314
                     /locus_tag="PKK03_08130"
     CDS             5006..5314
                     /locus_tag="PKK03_08130"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNX84407.1"
                     /translation="MIVTCVHVHVVPERVADFKDALKLNYLGTVGEPGNIRFDVLQQA
                     DDPCWFMIYEVFESEEAVRHHKETPHYLRWRDKVEQWMAEPRKGIKYNILEPSDRSLW
                     "
     gene            5311..6516
                     /locus_tag="PKK03_08135"
     CDS             5311..6516
                     /locus_tag="PKK03_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNX84408.1"
                     /translation="MKPFRLARIPQVLFRSGAISDLPDITKGYGRNTVLVTGARSFVS
                     SRRADRLFEQLRAGGLNVYHVTVAGEPSPELVDDAVSRLKGAGISLVVSIGGGSVMDA
                     GKAISAMMTTDGSVTDYLEVVGSREHPGTKVPFIAVPTTSGTGSEATKNAVISQVGEE
                     GFKRSLRHDNFVPDLVIIDPELTLECPPDITAAAGMDCFTQLTEAYLSDKGSPITDAL
                     AIEGLKAVSRSLLRSYTNGDDIEARSGMSYAALLSGICLANAGLGAVHGLAGTIGALF
                     NIPHGAVCGTLMAAANDLNVSELLKIIRYGSSTGDIAVTNANTALGKYSALGRIFADA
                     TGKSDSYYIGFFIDYLHAITYRFNLPRLGSYGLVYSDLEAISSQSDVKNNPVRLSSAR
                     LLEILGQRL"
     gene            6649..9069
                     /locus_tag="PKK03_08140"
     CDS             6649..9069
                     /locus_tag="PKK03_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX84409.1"
                     /translation="MLRNLIKYSVRSFKRQRSYIIINILGLSIGIACSLLIAIFVLNE
                     ASYDKYNEKKDRIFRLVLNGKIGGQEIVGAFTPSVMGPTMAEEFPEVEDFLRMTGSGP
                     TVVEYDAHIFTEDNLIQADSSFLDFFTIPVITGDPDQMLNAPHKAVLTESTAKKIFGE
                     ESPIDKALKIGSDSVRYIVTGVIGDIPENSHFDANIITSFMTNPRSKAPDWMSNSFST
                     YLLLNPNASASAVETKITDLLVKYVGPELQKYMGITIDEFIGQGNRYEYFLQNLEEIH
                     LDPSVQQEFKAASDPKYLIIFGSVAILIVLVAAVNFMNLSTAQASRRAKEVGIKKVGG
                     STRGMLVTQFLAESFILSFVSLLLALVIIKLSLPYFSNLLGTQMELKLLSCWYTIPAM
                     LLFTVVVALLAGSYPAMFLSSFNPYEVLKGSISGSMKNGRMRRVLVVFQFAVSILLIV
                     GTVVMYRQINFMLNKDVGFNKEQLIVIERAHVLGPRWKAFKDAAREIPGVVNIASSTA
                     VPGRNNNNNGYLIEGRNDESFLMVTNWVDPDYLDTYGMKIASGRFFSEAFTTDSEACL
                     VNESAVREFGLEQPDQTRIIRPGDEGDQLHMPVIGVVKNFNHESLRNPIQPYVFCFKG
                     DNQSWGYLTVRLEAQNYSATIAGIENLWKEFTANNPLQYYFVDDDFENMYRQEKQNAQ
                     MAVIFSILALFIAALGLFGLTSYTVEQRTKEIGVRKAMGSSVAGIYVVISREVIILVS
                     ISALLAWPLIWYFAGKWLESFHYRIELGVPVLLAGFVVALVIALMTISYRIIRAARVN
                     PAQSLKYE"
     gene            complement(9098..9406)
                     /locus_tag="PKK03_08145"
     CDS             complement(9098..9406)
                     /locus_tag="PKK03_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84410.1"
                     /translation="MTVKLYPNEVVIKAGDTNQLIENIKINGKLILTNQRIYFKTLSD
                     ETEKFNFEILFDNILEVTFYGKGIFAARGLQIVTRDGRNHSFPLRERDEIGKLINRMY
                     "
     gene            9587..10951
                     /locus_tag="PKK03_08150"
     CDS             9587..10951
                     /locus_tag="PKK03_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNX84411.1"
                     /translation="MVNGKILIVDRDKDSLRAMVRTLQGEFEKVIPVQSADRIRDTFR
                     QTEVDVVLLDSNFKSSVHNGNEGLFWMREVLNHDRNISVVIVTSSDESDLPVRAIREG
                     AFDFLRKPWEEARLLATVNAAWQLRHSKLEASSLRKDNIQLKEVINSGSEKIIRGASP
                     TMIRVMDTVKKVAATDANVLITGENGTGKELVAKEIHRLSGRSGELMVSVDMGSVTES
                     LIESELFGHVRGSFTDAREERVGKFEAANHSTLFLDEIGNLSLQAQSKILRVLQSRSV
                     CRVGSNRQIKVDIRLITATNCDIMKMVKEGRFREDLLYRINTIMIEVPPLRDRVDDIP
                     ILAKHFLRNYSSRYGRGDMRISIQGLEKLTTHDWPGNVRELQHAIEKAVILSDSPVLK
                     PSDFIFTPSPARSSHAEMTLEEMEKRFIAESLRRHNGNITATAGKLGITRQTLYNKMN
                     SYGL"
     gene            complement(11052..11717)
                     /locus_tag="PKK03_08155"
     CDS             complement(11052..11717)
                     /locus_tag="PKK03_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX84412.1"
                     /translation="MIKTNDLTKVFRTEEVETTALNKVCLEVKEGEYVAVMGPSGCGK
                     STLLNILGLLDNPTSGSYLFNGTEVANLRERDRTIFRKGNIGFVFQSFNLIDELNVWE
                     NVELPLIYLKMKTGERKKKVEDALERMKITHRAKHFPQQLSGGQQQRVAIARAVVANP
                     RLILADEPTGNLDSKNGIEVINLLSELNKEGTTIIMVTHSDRDAGYAHRIVNLFDGQV
                     VTV"
     gene            complement(11735..12994)
                     /locus_tag="PKK03_08160"
     CDS             complement(11735..12994)
                     /locus_tag="PKK03_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX84413.1"
                     /translation="MDKPIEKKKGIRGKHIAWIAGAIAFIVLMYMAFFAKRTSTFRVE
                     KDKLIIETVTENVFNDYITVPGTVEPISTIFLDAQEGGRVEEILIEEGSMVSEGDIIL
                     KLSNPDLYLNILDSEAQLAEKENFLRNTQIAMEQDRLQIKRELLNLKYDIERKKRNYD
                     QNRMLMHDSLISVEEFIRSKEDLDMAIQSEELFVERQKQDSVFRSVQVDNLKNNLDNM
                     RKNLALVRDRMENLNVRATVDGQLGLLVPEIGQSISRGSNMGQINVLTSFKVTAQIDE
                     HYIDRIRTGLTATLDRQGNAFNLTVRRVYPEVRNGTFKIDMVFTDTVPGNIRTGQTYY
                     TSLQLGQPKTAILVPIGGFFQETGGQWIYVVDPSESYATRRDISTGRKNPKYYEVLEG
                     LQPGEKVIISGYETFGNNEKLVFKNSK"
     gene            complement(13033..>14325)
                     /locus_tag="PKK03_08165"
     CDS             complement(13033..>14325)
                     /locus_tag="PKK03_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX84414.1"
                     /translation="AQETKWTLSDCITYALENNAGLQRQVLLTETAAVNLTKSKMDLL
                     PDLNLGSDARVGFGRSIDPVTNLITFKQNVSNSYSLNSSVELFNGFTTLNTIAAGKFM
                     LKAGLESEKVARNTLIVDIMGQYYQVTYAKGLEAAAKMQMELSERQLFRITRMVETGK
                     ESVSKKYEMESQASGDRLTWTIARNNASQAVTQLKQMLQLQSGVEFDIQLPDLDLIMI
                     SNTGYETDSVYNVAARVLPRLRAIEYELEAAERQLAAAKGQLAPGLSAGGAIYTGYYK
                     VLGENTEDQASFASQLKNNNSQAVYLSLNIPIFNRYSATRNIKLARIRKSDTELRLEL
                     EKNNLYTEIENACLSYNRGKQEYLAAEANLEFNRKSYNAVEKKFESGLVDVTDYSAAR
                     TTLFSAETEALRTKLQLVIRELTIRFFLTGEYENPAIN"
BASE COUNT         3608 a         3305 c         3643 g         3769 t
ORIGIN      
        1 gaaaaaaaca atgaattcac ccttctggga cagggatcct atgaactgaa aatagggtca
       61 gatgaggttt ataccataaa gatatggaag gagggattcg agccttttgt gaaatcctac
      121 accaggacga acaatgggac actgacagag aatgttgaac tccaaaaccg ggtggtgaaa
      181 gtcgtggcct ctcccaatac caccgaggtt tatgctgata atgtttatgt tggcaaggga
      241 ggggaggagc ttgaggttgt ggtacggtat aacaacatgg tcaatctgga ggtcagggga
      301 gaggggtatc agactgtaag gcgctcgtat tataacatgg aaaatacaga gatcccgccg
      361 ctgaaggaga acattgagct ggcagaaaga cttgtaaaga ttctggccag ccctcccgga
      421 tgccttatat ctgttgacgg gaagcctgtc ggagagacct cggccgaggt tgttgtcccc
      481 aaaggatcat gcataacgct caaggtggtc aaggatgggt atgccccctt tgaaaagtca
      541 tattgcaaca agcccgaagt agcccttccg ccgatatccg aggaaattat tctgaaggac
      601 agaatcgtgc aaataaatac cactcccgag gatgcagcta tcagggttga tggcagggca
      661 gtgggtaaag ggaactattc gttgctggta aaacgtgatc agtgtgctga gatcgaggtg
      721 atgaaagacg gctttgacac aaacaagaag tcatattgca acaaggcaaa catgagtgaa
      781 cccccggtaa cggatcacat caggcttgcc gaggatgagg cgtggctttc atcaatcaga
      841 agcgatcagg ccaatgtcaa cttttcaata cctgttaatt ccagccttgg taatgatgct
      901 gcatggaaaa ttatcggaca gattgttatg gagaagttcg atgtgatcga aatcagtgat
      961 ccagttacgg gttacctgag gactgcgtgg aatgtgaagc ttttcgggaa cggtaagacc
     1021 atccggaccc gttgcatagt taagctggga aattcgaatc cattgcgtta cgtgatcaag
     1081 atcgtcagcg aggagagtat tgttccgggc accagtgtga aggaagacga aaacttcaag
     1141 gaatgggaca ggatactgaa tacctataaa gacataatag gagaaattca ggcgagaatc
     1201 agctgagatc cggtgtggca atcccgtatc tctccggtac taaaaaataa tcttaatccc
     1261 cgctgtcacg ggagtgatgg caattgtcag gcttggacca gatgcaatac cggcagacga
     1321 atgtgaatta tagaggttaa ttgacctgac aaactgggca ttgcctttaa gcaggaaggg
     1381 aatgcccgcc agtgccatgc cgcaacctat cagaaaagga cctgtgttgg cgccggatac
     1441 atcaaggctg ccactgttaa gtgtctcaag atcattaagg gtgctgatca gtgaggatgc
     1501 tcctatcacc agacaaccac cgccaaggag accgagacca ataccggaat tcctggatga
     1561 ttttttcaat tcatcggctg atccagtata gcctgacaga acggttcgta tttccttccg
     1621 gtcaagacgc tggccgttct gaaaatactg gatggccaac ccctttttct cagtggttat
     1681 cggacctggt tgctgcgctg cggccgacat gcacgaaatg gtgcagatta cggcaattat
     1741 gacacttctt atcatacgga ttcgttttta aaacattgat aacaggtatt atttatgacg
     1801 aaaagttagg taatattatt aaatatttaa tatattagcc tgacaaaata atattaatcc
     1861 tgagctgttt ttggcagggc cggagagaaa gagtttaatt attagctgaa gtgttcaggt
     1921 gaagtttctc ctgaacctgg tttaatgcgg agtaaacaaa aagctatagt ccttttcaaa
     1981 taactaaaat gtcagcagga tgagaacaaa gaattattta attggccttg tcattggggt
     2041 gttgatcttc tctgtttcaa ctctggaaac caatgctcag agtcagtatg ccgcttggcg
     2101 tgaccgcagt gatgagctgc ccgggatgat cagtgacggt gaaatgttgc tgattgcggg
     2161 tggagcagta ctggttatcg gaggtgtgat aacatatatt attatcaaaa agaagcagga
     2221 caacaagctt gaggcaatgt tgcccgactg ctgtggtatg acaggaactg aaaggattgt
     2281 ttctggccgg acacagggca gctcacttgg atcactttac aatgacatct ccagggccac
     2341 ggaccaggca tcagtgctga tcttctcaag gctggacaag atacctaatc cgggtcaggg
     2401 acagaacaat gttctttcaa tgggggtatt gattaagttt tagcggttat ttggatagga
     2461 ctgggtgcat ctctgtaaaa aaaaagacgc ccgttcaggg cgtcctatgg aattccgata
     2521 caaagatcag gctaactatt gacatttgag gtttgtcctc tgtgcctttg ctgcctctgc
     2581 aaacggaccg tagagtgcat tcttgaatga ctcgcatgcg gcagcggtgt cacccttgcc
     2641 cacctgtgca acggcgagct gataatagaa cattgccttg gcttctgcgt cacctgtctc
     2701 cagatccagc cctttctgtg caaactcggc accttcggtg aagctgtttt tcttgttata
     2761 gacatcggca aagaaataga acatggtctt gtcgtcacca tactttgctg ctctgttcag
     2821 cagagccagc gcttcgtcga gtttattggc actgttggct tttgatcctg ctgcgcgaaa
     2881 atactcaagt gcggcgcctt tagcctgtac ggccttgtcg gcatccttcg cgggatcaag
     2941 cttagccagg aactggtcga tggtctgctc gaagagatca gcctcatcac ggttcttata
     3001 aataagagcc ttgttataaa tagaggcaat gtgatcagga ttcagcgtca gcacactgtc
     3061 aaatgcgagc atcgcattgt catagtcatc ggcagagaag aattcggttg ccatcttgta
     3121 gtaaccgttg acaaggatct tttttgaatt ctctccgtgg gttttgctcc cgtacttttc
     3181 agccatggca cctgcaagtt tcgaggctgc gattatctcg gcagcaggtt tcttttcgtt
     3241 gtaagcagtg gcagcaagct tatagtatag tccgggtagt acctgtacgg ccttctgttt
     3301 cagatcattg gtagtttcac caaccttgtc ggacagttca atcaccttct cgaaagcagt
     3361 tatcgctgtc ggaacatcag tctggatcgc cttggcgcct tcattgtaaa ctgctataac
     3421 ctcattacgg tcctgtgcat tcaggccgga ggcaaagaat gctgaaaaag ccagcacaca
     3481 agctattgaa cataaaagtt tcatcttcat tttcagttta ttttacgaat tatcaaaaat
     3541 caggcctttt attagcccgt aaatataaaa aatatgatga attattcagg atacatgtaa
     3601 aattcacaat aaaaaaacgg atcgtgaaga ttgaaattta cggattgcag cgtaaaaccg
     3661 gtcatgtgaa gaaacagttt gaactcaatg ttaaataaac atccggaata aaatgtattt
     3721 ttaaacctct caaaaatctg cgggacatgc cagaaagctt cgggaaaaag cctgaaaaag
     3781 gaaatgtggt cagggttgca tgggcggctc acagaggtgc tccggcatcg actgctgctg
     3841 aagcggaaat gcagatacgg cattttttgg cgcctgatga ttttataata accggcaccc
     3901 gccctgacat aattcttttc atgagcggcg gatcggagag aagggccatt gaactggcag
     3961 atcaggggaa gcctgtgctg ctgctgagca tttgtggcaa caacgcctat gcttcagcca
     4021 ccgaggtgat ggcatggatg gtgaacaaca acaggatggc cattttatcc gacctgtccg
     4081 aggcggcaga gtcaggcctt ttaaaccggt ggtgcaggct ggcaggcgca tgggagaggc
     4141 ttgacggagc cagggcagga ttgataggtg ccgtctccga atggctggtg gcttccggtg
     4201 tttccgcaca aaccttactg agcagattcg ggatcagcct gacagaaatt ccgtggtccg
     4261 gattgcctga ttacacttca tcacagcctg accaggccct gatgaacagg ttcgggggcc
     4321 atagccctga gggtcttgat gaggctgcga gggtgctgac actcctccgg aaggtaatag
     4381 atgagaaccg cctggatgcc gttggtgttg agtgcttcag cctggtgcag gagcggaagg
     4441 ttacggcctg cctcgccctg gctcagctta acacagaagg aaccgtggca gcctgcgaag
     4501 gagaccttgc atcgatggcc ggcatgatgg ccgggaaggc cctgaccgga cgcgttccat
     4561 ggatggccaa cactacccgc ctgaccggaa aatcagtgat tctttcacac tgtaccgcac
     4621 cattcgatct tgtgagtgat gtgcagttgc tgtctcacta cgagacgaga ttgtcacttg
     4681 ctgtggatgg caaaatcaga gaaactgagg taaccttatt ccggttctcg gacatgcttg
     4741 acagggcttt tgtagccgat ggcagaatta tcagccatcc gaggatcgca aatgcctgcc
     4801 gcactcaggt ggagacagag attgacaaaa aagccctgca attgctgaag gagaaacctc
     4861 tgggcaatca tttgctattg ctccccggca atcattcaga tttcctgcga cttgcatgca
     4921 ggtacaaagg gattgaaaca ataagttagc tggtcttatg gaacatatgt tcctgtgagt
     4981 tggatattaa tatgggagaa taaatatgat agtcacatgc gttcacgttc atgtagtccc
     5041 tgaaagagta gctgatttca aggatgccct taaattaaac taccttggga cggtgggtga
     5101 gcccggcaac atcaggtttg atgtgttgca gcaggccgat gatccttgct ggttcatgat
     5161 ctacgaggtc tttgaatcag aggaggctgt caggcatcat aaggagactc cgcattatct
     5221 ccggtggagg gataaggtgg agcagtggat ggccgaacca cgaaagggca taaaatacaa
     5281 cattcttgaa ccttcagaca ggtcgttgtg gtgaaaccgt tcaggcttgc caggatccct
     5341 caggttttgt tcagaagcgg tgcaatcagt gacctgccgg acattaccaa aggctatggt
     5401 agaaacactg tcctggtcac cggggccagg tcatttgtat catcccgccg cgctgaccgc
     5461 ctgtttgagc agctcagggc cgggggacta aatgtctatc atgtaactgt cgccggggaa
     5521 ccgtcgcctg agctggttga cgatgctgtc agccggttga agggagccgg tatctcactc
     5581 gttgtgagca taggcggggg cagcgtcatg gatgccggga aagccatatc ggccatgatg
     5641 acaactgacg ggtcagttac tgactacctt gaggtggtcg gcagccgtga acaccccggg
     5701 acaaaagtcc cgttcattgc agttccgaca acctcgggta ccggcagcga ggccacgaaa
     5761 aatgcggtca tttcgcaggt gggggaagag ggcttcaagc gctcgctgcg tcatgacaat
     5821 tttgtgcctg acctggtgat cattgatccg gaacttaccc ttgaatgccc gcccgacata
     5881 actgctgctg cggggatgga ctgttttaca cagcttacgg aggcatatct ttccgataag
     5941 ggcagcccca taaccgatgc ccttgccatt gaggggctga aggcggtcag ccggtcgctc
     6001 ctgagatcat atacaaacgg tgatgatatt gaggcaaggt ccggtatgtc atatgcagcc
     6061 cttttgtccg gcatctgcct ggccaatgca gggctggggg cagttcacgg actggccgga
     6121 accatcggag cgcttttcaa tattccgcac ggcgcagtct gcggcaccct tatggctgcc
     6181 gccaatgatt tgaatgtgag tgagctgctc aaaataatcc ggtacggttc ctccacggga
     6241 gacattgcag tcacaaacgc aaatacagct cttggaaaat actcagccct gggcaggatt
     6301 ttcgcggacg ccaccgggaa gagtgacagc tattacatcg gttttttcat agactatctg
     6361 catgcaatca cctacaggtt taacctgcca cggctgggca gctacggcct ggtatacagt
     6421 gacctggagg caataagttc acagtcagac gtgaaaaaca atcctgtgag actctcctct
     6481 gcccggttac ttgagatact tggacagagg ctgtaacagc gaatacgtgt gtcttgcaga
     6541 caaggctgtg cctgcaggta ccgtcccggc ataattttat agttctatgc agcgggaatg
     6601 cctggccagc gtctttacca agagaataaa agataaaccc gacctgcaat gttaaggaat
     6661 ctgatcaagt acagcgtacg ctctttcaag cgtcagagat catacataat cataaatata
     6721 ctgggccttt caatagggat agcatgcagt ctgctcattg caatttttgt gcttaacgaa
     6781 gcaagttatg ataagtacaa tgagaagaag gacagaattt tcaggctcgt acttaacggt
     6841 aagataggcg gtcaggagat tgttggcgca ttcactccat cagtcatggg tcctacaatg
     6901 gctgaggaat tccccgaggt ggaggatttt ctccggatga ccggttcggg tccgacagtt
     6961 gttgaatacg atgcgcatat ttttacagaa gacaatctca tacaggcaga ttcatcattt
     7021 cttgactttt ttacaattcc ggttatcacg ggagaccctg accagatgct taacgcccct
     7081 cacaaggctg tcctgacaga gtcaactgca aaaaagattt tcggtgagga atctccgatt
     7141 gacaaggcgt tgaagatcgg ttctgattct gtccgttata tagtcacggg agttattggt
     7201 gatattcctg agaattccca ttttgatgca aacattataa cctctttcat gacgaatccc
     7261 cggtcaaagg cacctgactg gatgagtaac agcttcagta cctaccttct tctcaatccc
     7321 aatgcaagtg cttcggctgt tgagacgaag atcacggatt tacttgtcaa gtatgttggt
     7381 cctgagctgc aaaaatacat gggaataact attgacgagt tcattggcca gggcaacaga
     7441 tacgagtatt tccttcaaaa cctggaggag atacatcttg atccctccgt acagcaggaa
     7501 ttcaaggcgg ccagtgaccc gaagtatctg attatcttcg gaagcgttgc aatattaatc
     7561 gtgcttgttg cagcagttaa ttttatgaac ctgtccaccg cccaggcttc gaggagagcc
     7621 aaggaagtag gaatcaaaaa agtaggagga tcgacgcggg gaatgcttgt cacccagttc
     7681 ctggcggaat cttttattct ttcattcgta tcactgttgc tcgcgcttgt aattatcaag
     7741 ctaagcctgc catatttcag caatctcctg ggaacgcaga tggagcttaa gctgctgtcc
     7801 tgctggtata caataccggc gatgctgctg ttcacggttg tggttgcact gcttgccggc
     7861 agctatcccg ccatgttcct ttcttcattc aatccgtacg aggttctcaa gggaagcatc
     7921 tccggcagca tgaagaacgg caggatgagg agggttcttg tcgtattcca gttcgcggtc
     7981 tcaatattac ttatagtcgg aacagttgtg atgtacaggc agattaactt tatgctcaac
     8041 aaagacgtag gtttcaacaa ggaacagctg attgttattg aaagggcaca tgtgctggga
     8101 ccgcgctgga aggcattcaa ggatgcagcc agggagatcc cgggcgttgt gaatatcgca
     8161 agttcaacag ctgttccggg caggaacaac aataacaacg gttaccttat tgaggggagg
     8221 aacgacgagt cattcctgat ggtaaccaac tgggttgacc ctgattacct tgatacttac
     8281 ggtatgaaga ttgcctcagg caggttcttc agtgaagcct tcacgacaga cagtgaggca
     8341 tgccttgtta acgaaagtgc tgtaagagag ttcggactgg agcaacctga tcagacacgg
     8401 atcatcagac cgggcgatga aggcgaccag cttcacatgc cggtaatcgg ggttgtaaag
     8461 aactttaacc atgaatccct gcgaaatccg atccagccat atgtcttctg cttcaaagga
     8521 gacaatcagt cgtggggcta cctgactgtc aggctggagg cacagaatta ttcagctact
     8581 atagctggta ttgaaaatct ctggaaagag ttcacagcca ataatccttt gcaatattat
     8641 tttgttgacg atgactttga aaatatgtac aggcaggaga aacagaatgc acagatggca
     8701 gtcatcttct cgattctggc cctgttcata gcagccctgg gtcttttcgg actgacctca
     8761 tatacagtcg aacaacgcac caaggagatc ggggtcagaa aggctatggg atcttcagtg
     8821 gcaggaatct atgtcgtgat atcgagggag gttatcatac tggtctccat atcagccctc
     8881 ctggcatggc ctctgatatg gtactttgcc ggaaaatggc ttgaaagctt ccactacagg
     8941 attgaactgg gagtgcctgt gctgctggcc gggtttgtcg ttgcacttgt aatagcgctg
     9001 atgactatca gctaccgtat aatcagggct gccagggtca atcctgcaca gtcactgaag
     9061 tatgagtaaa tgaaatcgtg cgggggataa tccgcaatca gtacatcctg ttgatcagct
     9121 tcccgatctc atccctctcc ctgagaggga atgagtggtt tcggccgtct cttgtaacta
     9181 tctgcaaacc tctggctgca aatatcccct tgccatagaa tgtgacttcc aggatattgt
     9241 cgaataatat ctcaaaattg aatttctctg tctcatctga gagggttttg aaatatatcc
     9301 tctggtttgt aagaataagt ttaccgttga tcttaatgtt ttcaatcagc tgatttgtat
     9361 cacctgcctt gatcacaact tcattggggt atagtttaac tgtcatagcg ccctggtttt
     9421 tcagttttgt ctgtcagtcc gtccggtcag gctgacatac aaaagactca ggcaaagccg
     9481 cggatgctgc atgcagtaac ccggtggcag ttcattttct cctgaaaaaa tgtccattaa
     9541 attgacacat tcccgaatat gtgccgtctt tagagaaacc gcatccatgg taaacggtaa
     9601 aatactcata gttgacaggg ataaagactc actccgggca atggtgagga ccctgcaggg
     9661 cgaatttgaa aaggttatcc ctgttcagtc tgctgaccgt atccgggaca ctttccggca
     9721 gacggaggtt gatgttgtac tgctggattc aaacttcaaa tcatcagtac acaacggaaa
     9781 tgagggactt ttctggatga gagaggttct taaccatgac aggaatataa gtgtagtgat
     9841 agttacctcc tcagatgaaa gtgaccttcc agtaagggca atcagggaag gggcctttga
     9901 tttccttcgc aagccctggg aggaggcaag gctgcttgca actgtgaatg cagcatggca
     9961 gctcaggcat tcgaagctgg aagcatcatc actcaggaag gataatatcc agttgaagga
    10021 ggttataaac agcgggagtg agaagataat caggggtgct tcgccaacga tgataagggt
    10081 aatggacacc gtgaagaagg ttgccgccac cgatgccaat gtccttataa caggagagaa
    10141 tgggacgggt aaagagttgg ttgccaaaga gatacacaga ttgtcaggac ggtccgggga
    10201 gttgatggtc agtgtagaca tggggtcagt cactgagtca ctcattgaga gtgaactctt
    10261 tggccatgta agaggttctt tcacagatgc cagggaggag agggtaggga agttcgaagc
    10321 cgccaaccac agcaccctgt tcctggatga gataggaaat ctgtcacttc aggcccagtc
    10381 aaagattctg cgggtgttgc agtcccgctc tgtctgcagg gtcggatcca accggcagat
    10441 caaagttgac attaggctta tcaccgcaac gaactgtgat ataatgaaga tggtaaaaga
    10501 gggtcgcttt cgcgaggatc tactttaccg gatcaatact ataatgattg aggttcctcc
    10561 attgcgtgac agggttgatg atattccaat cctggcgaag cattttctgc gcaactacag
    10621 ctccaggtat ggcaggggcg atatgaggat cagcattcag ggccttgaaa agctgactac
    10681 ccacgactgg cctggcaatg taagagagtt gcagcatgca attgaaaaag ctgttatact
    10741 tagcgactct cctgtgctca aaccgtctga ttttattttc accccctctc ctgcaaggtc
    10801 atcccatgca gagatgacgc tggaggagat ggagaaaaga ttcattgctg agagcctgag
    10861 gaggcacaac ggtaacataa ccgcaacagc cggtaaactg ggtataacaa ggcagacgct
    10921 ctataacaag atgaattcct acgggttgta gtgagccgga cctccggcac actggcccat
    10981 ggatcctctg ttcctcatgc tctcaggatc cagggctaaa aggctcccgg ccggaatgag
    11041 cagattccag tctaaacagt aaccacctgt ccgtcaaaca gatttacaat cctgtgggca
    11101 tagcccgcat ccctgtcaga gtgtgtcacc attatgattg tcgtaccttc cttgttcagt
    11161 tcggataaga ggttaataac ctcaataccg ttttttgaat ccaggttgcc cgtaggctca
    11221 tccgccagga tcagccgggg gttggcaacc acagcccttg caattgccac cctctgctgc
    11281 tggcctcccg acagctgctg cggaaagtgt ttggcacggt gagtgatctt cattctttca
    11341 agcgcatcct caaccttctt tttcctctcg ccggtcttca tcttcagata gatcagcggt
    11401 aattcgacat tctcccagac attcagttcg tctatcaggt taaagctctg gaagacaaac
    11461 ccgatgtttc ccttcctgaa gattgtacgg tcacgctcgc gcaggtttgc cacctccgtt
    11521 ccgttaaata gatagcttcc ggatgtggga ttgtcaagca gaccaaggat attcagaaga
    11581 gttgatttgc cgcatccgga aggacccata acggccacat attcaccctc tttcacttcc
    11641 aggcagactt tgttgagcgc tgtcgtctcc acctcttccg tacggaagac ctttgtcaaa
    11701 tcatttgtct ttatcatgat tttaatattg gttattattt gctgttttta aataccagtt
    11761 tttcattatt cccgaaggtc tcatatcctg agattatgac cttttctccc ggctgcagtc
    11821 cttcaaggac ctcatagtac tttggatttt ttcttcctgt tgagatatct ctccgggttg
    11881 catatgattc tgaaggatca acgacgtata tccactgccc tccggtttcc tggaagaagc
    11941 cgccaatagg tacaagtatg gctgttttgg gttgtcccag ctgaagactt gtataatagg
    12001 tctggccggt acggatgttc cccggcacag tgtcagtgaa caccatatct atcttaaagg
    12061 ttccgtttcg cacttcgggg tacaccctgc gcaccgtcag gttaaaggca ttgccctgcc
    12121 tgtcgagggt tgctgtgaga ccggtacgga ttctgtcaat atagtgttca tctatctggg
    12181 cggtcacctt aaaggatgta agcacattga tctggcccat atttgagccc ctggagattg
    12241 actgaccgat ttcgggcaca agcaatccaa gctggccgtc aacagttgcc cgcacattca
    12301 ggttctccat ccggtccctg acaagcgcca ggtttttacg catgttatcg aggttattct
    12361 tcaggttgtc aacctgaacc gagcggaaga ccgaatcctg tttctgcctt tcaacgaaaa
    12421 gctcctctga ctgaattgcc atgtccagat cctcctttga gcggatgaac tcctccacag
    12481 agatgaggct gtcatgcata agcatccggt tctggtcgta gttccttttc tttctctcga
    12541 tgtcgtactt tagattcagc agttcgcgct tgatctgcaa tctgtcctgc tccatggcta
    12601 tctgtgtgtt cctcaggaag ttttcctttt cagccagctg tgcctcgctg tcgagtatgt
    12661 tcagataaag gtcagggttt gagagtttca ggatgatgtc tccctcactg accattgatc
    12721 cctcttctat gagtatttcc tcgacgcggc ccccttcctg tgcatcgagg aatatcgtgc
    12781 tgattggttc cacagtgccg ggaacggtta tgtaatcatt gaatacgttc tccgtaacgg
    12841 tctcaattat aagcttatcc ttctccaccc tgaaggttga ggttcgcttt gcgaagaatg
    12901 ccatatacat aagcactatg aatgctatgg caccggcaat ccacgcgatg tgttttcccc
    12961 ttatgccctt cttcttctca atgggtttgt ccattctttc aagcggcttg tccattctgt
    13021 tttattttga gtttagttta ttgcaggatt ttcatactca cctgtcagga agaaccggat
    13081 ggtcagttca cgtatcacca gttgcagttt ggtcctgaga gcctctgtct ccgccgagaa
    13141 cagggtagtt ctggcagcag agtaatcagt aacgtctaca agtcccgatt caaacttctt
    13201 ttcaacggca ttgtaagact tcctgttgaa ttccaggttg gcttcagcag cgaggtactc
    13261 ctgctttccg cgattgtaac tcagacaggc attttctatc tcagtataga ggttgttctt
    13321 ctccagttcg agccggagtt cagtgtcact cttgcggatc cttgccagtt tgatattcct
    13381 ggtggcggaa tacctgttga atatggggat attcagcgag aggtacacag cctggctgtt
    13441 gttgttcttc agctggctgg caaaggaggc ctgatcttcc gtattctcac caagcacctt
    13501 atagtaacct gtatagatgg ctcctcctgc agagaggccg ggagccagct gacccttagc
    13561 cgcagcaagc tgtctttccg ctgcttcgag ttcgtactct atggccctga gcctggggag
    13621 aacacgggct gccacattgt atacgctgtc tgtttcgtat ccggtattgc tgatcattat
    13681 caaatcaagg tcagggagtt gaatgtcaaa ctccacacct gactgcaact gaagcatttg
    13741 tttcagctgt gtcaccgcct ggctggcgtt gttccgggct attgtccagg taagcctgtc
    13801 gcctgacgcc tggctctcca tttcatattt ctttgaaacc gattcttttc cggtctccac
    13861 catccttgtg atcctgaaaa gctgtctctc cgacagctcc atctgcatct tcgcggctgc
    13921 ctccagccct ttggcgtagg tgacctggta atactgcccc ataatatcaa ctataagtgt
    13981 attgcgggca accttttccg actccagccc tgccttcagc atgaatttgc ctgcggcgat
    14041 tgtgttcagg gttgtgaacc cgttgaagag ttcaaccgac gagttcaggg agtatgagtt
    14101 gctgacattc tgcttgaatg tgatcaggtt ggttacaggg tctattgatc tcccgaatcc
    14161 tacccttgca tcggagccca ggttgagatc gggaagcaga tccatctttg actttgtcag
    14221 gttcacagcc gcagtctcag taagtaaaac ctgtctctgc agccctgcat tattctcaag
    14281 tgcataggtt atgcagtcac tcagagtcca ttttgtttcc tgggc
//
LOCUS       DAOPXU010000070        14193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_4664_length_14193_cov_11.197765, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000070 DAOPXU010000000
VERSION     DAOPXU010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14193
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4664_length_14193_cov_11.197765"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..3223)
                     /locus_tag="PKK03_08170"
     CDS             complement(140..3223)
                     /locus_tag="PKK03_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84415.1"
                     /translation="MSSCKKNTISVRVIRLLFAVLTAWLITGVSSDAQYFGRNKPGYS
                     SFRFDVLQTPAFEIYHYMKNDSLLKTISGWSEEWFSIHQKIFRDTFERKNPLIFYNNH
                     SDFQQTNAVSGLIGTGTGGVTEGMKNRVIMPVALTLSQTDHTLGHELVHAFQFNMFLR
                     SDTTQKYSMNNIPLWMIEGMAEYFSLGSIDPNTAMWMRDAVLNKDFPTLKQLSSDSKY
                     FPYRYGQAFWAMVGKTWGDTTIMPLFRKTAQYGLSKAADTILGMDEKTLSGAWASAME
                     VYYRPYLKADADELAGKQLITTENGGRINISPAISPDGKYVAFFSEKNIFTLDLFVAD
                     ASTGKIISKVSGAAARTYDDFNYVESGGSWSPDSKKYVFVLFSDGINKLAFVDIKRGR
                     ITREVAMRGVPAISNPSWSPDSKKIVFTGSSEGISDLYLYDLMSGDIEKLTDDFTSNI
                     HPSWSSDSRTIVFSQEKMNASGHRKFSYSLAMLDLSTRKVSEIDVFEGAYNMNPVFSA
                     DDSHIYFLSDADGFRNMYKYEIATGRLLRLTSYMTGISGITPWSPAMSWSRDGNKTAY
                     NYYIKNSYHILVADGDEFRAEEADGSVVNFDAGTLPPLEHLSVNLIDSALYARKEVVQ
                     LPADEVKELPYKPRFKLDYISNNASVGVSSGIYRNNLGGSVNMIFSDMIGDNQLYSSL
                     SLNGEIYDFAGQAAYVRQKGRIKWGTAVSHIPYRAGSMAFGKDSVLLQGEMVPVDVLQ
                     LDYIRMFEDNITLYASLPFSQVRRIEATASASWYYYRIDRFNYYYLLNGYNIGGTREK
                     MPAPEGSSYQQLSFAYVKDNSLFGMTAPMQGGRARFQVDKYFGATQIFTGLIDYRQYI
                     YRKPFTFAFRLYNYGMYGKVTEENVLQPLYLGYPWLIRGYENISYNTGDTPGLASLNI
                     SRLNGTRMAVANAEIRFPFSGPERLALIKSKWFLTDLNLFFDAGMAWNRGDKIIFNDQ
                     ESHPADNEKFPLYSTGASLRINLFGYLILEPYYAFPFQNGGFKNGVFGLNFVPGW"
     gene            3429..5495
                     /locus_tag="PKK03_08175"
     CDS             3429..5495
                     /locus_tag="PKK03_08175"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNX84416.1"
                     /translation="MKKLSIITLLLLMALMGACKTEKTEKAATPAIDVSLTDAEKAGG
                     VLTPEIMWKYGRIGTIALSPDGSTVLYQVTNYDLPTEARATNIWSVPASGGDPVQLTT
                     EGGAGPQWIENGARIAFLAGGKIMVMNPDGTGKKEITGLEDFEILSFSPAGDKVYFTR
                     RVKLDQTANEKYGLPNAKVRIINDLMYRHWDYWHDYSYSHVFVASFDGTAISNEKDIM
                     EGQRFDAPDAPWFDEADLAWSPDGKYLAYACKKLTGKAYSLSTNTDIYLYDVTTGGEK
                     NITEGNMGYDKTPVFSPDGSMIAYSSMEEDGYESDLERLFVYNIASGERKWVSQGWDY
                     NVGNIQWNGSAEIWFTSAYLGRQPVFKISLADGAVTKVTEGDYDMGPLQLRNNTLVAG
                     IQSMARPTEVAKVDMAGGQFSMITEVNGHIYESIKMGKSEERYIKTKDGKDLQMWIIY
                     PPDFDPSKKYPALLYCKGGPQGPLGQSWSYRWNYQVMAANGYIVVAVNRRGNSGFGSY
                     WREQISGDYGGKNIQDYLDGIDAMAKEPFVDKDRLGAVGASYGGYSVFYLAGMHNKRF
                     KAFVAHCGVYNTVAEYGSTEEYWYPNKDYQGAPWDKPQPPGYNKFSPHTMVDKWDTPI
                     LIITGANDFRIPYSQSLEAFNSAQLRGVPARLLFFENESHWVTKPQNSVIWQKEYFEF
                     LDSYLK"
     gene            complement(5610..6428)
                     /locus_tag="PKK03_08180"
     CDS             complement(5610..6428)
                     /locus_tag="PKK03_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNX84417.1"
                     /translation="MENNNLFDKLPMAGIKNIIVVASGKGGVGKSTVAANLAIALARN
                     GMKTALVDADIFGPSIPTIFGLEDVRPRVESAGGKDVMYPVEKFGIKIISIGFFIDRS
                     QSLIWRGPMAANAVKQLFEDTQWGEIDYMIVDFPPGTSDIQLTTVQKLNLSGAIIVTT
                     PQEIALNDARKASSMFMNPDLQVPILGVVENMSWFTPAAHPEEHYYLFGRGGGAKLAK
                     EYDTNLLCQIPLVMEVGEAAEAGRTVFQQTNKTAVEAFEILAGQVMEVTENLKV"
     gene            complement(6418..7287)
                     /locus_tag="PKK03_08185"
     CDS             complement(6418..7287)
                     /locus_tag="PKK03_08185"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX84418.1"
                     /translation="MELAVISGKGGTGKSSVTAAFATVGERLVVADCDVDAANLYLIF
                     NPEHNNEQVYIGGHKAVVDHTLCTLCGICEGYCRFDAIKEINGKIVISETSCDGCFFC
                     SRICPMNAITMVRSDKSRMYSGSFRNGIMVYGRLAPGEENSGKLVSMVRDEAKRVATE
                     QGVPVILLDGPPGIGCPVISTVTGVDRMIIVTEPTMSGLEDLKRVFEVARKFTGKVGI
                     IINKCDLNHAMADRIEGWCTAHGASLLGRLPYDSRVTEAMVAGRSVTEMFPDAEISNI
                     IKDIWNNLSTNGK"
     gene            complement(7262..8134)
                     /locus_tag="PKK03_08190"
     CDS             complement(7262..8134)
                     /locus_tag="PKK03_08190"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX84419.1"
                     /translation="MKIAVASGKGGTGKTFVATNLFNVLSRRGEAVTLTDCDAEAPDD
                     LLFFRAEKVSETEVTHQVPVINKDACTWCGRCSRWCNYNAIFYVPAAKVIEVIENLCH
                     GCGACTFACNSGAITEKPVLLGKVTKFMTDGGSKVVEARMKVNEMSPVRIIKEAIKES
                     GEDGVVILDAPPGTSCPFIQTVDKADNVLLVTEPTPFGLSDLRQSVETLREMKKTPAV
                     IINRSDIGDGSVAEYLAKENIPLLAEIKYDPAIARVYSEGKLVTDEIPALTSLFEDMA
                     DKIIEGYGTGSNQR"
     gene            complement(8147..8590)
                     /locus_tag="PKK03_08195"
     CDS             complement(8147..8590)
                     /locus_tag="PKK03_08195"
                     /inference="COORDINATES: protein motif:HMM:NF020400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HNX84420.1"
                     /translation="MEKKRIGIIICDRYHTCAGGKCFRSFRNREGAFDRYMDEDVEIA
                     AFTTCGGCPGGNVEYTPEEMIKNGVSVIHLATGLVVGYPPCPRLDYFTRFIPEKYGIE
                     VVVGTHPVPQKYYTTHMNLGTWKGEAMQRFIKPALADEKMRLAYD"
     gene            complement(8600..8938)
                     /locus_tag="PKK03_08200"
     CDS             complement(8600..8938)
                     /locus_tag="PKK03_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNX84421.1"
                     /translation="MKKRIAIPLEGGILCSHFGHCEQFAIVDAEDKTITAKTLITPPP
                     HEPGLLPGWLAEKGITDVIAGGMGQRAIGLFNQHSINVFVGAPVKSPDELAADLLNER
                     LQAGANYCDH"
     assembly_gap    9097..9196
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9462..9782)
                     /locus_tag="PKK03_08205"
     CDS             complement(9462..9782)
                     /locus_tag="PKK03_08205"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNX84422.1"
                     /translation="MKVAITSTGQSLESKLDQRFGRCAFFVIYDTETGGVEILPNPNK
                     DAEEGAGPASVQLVASKEVEKIVSGEFGYKIKSLLDSLRIQMVVYKEPEKTVKSVIDM
                     LNKQ"
     gene            complement(9794..10378)
                     /locus_tag="PKK03_08210"
     CDS             complement(9794..10378)
                     /locus_tag="PKK03_08210"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNX84423.1"
                     /translation="MSPRPTRLRRVSNPPVISGFKPYGGMAKVGQPRSVFLHLEEYEV
                     IRLCDFENLNHHEAARVMDVSRPTLTRIYAKARAKIADALVTGKQIIIEGGKIYFDSE
                     WYKCNVCGCLFNQPEKSVTPEKCPLCGSGSIVNYVPVDDSTQVTRRAPSDICVCPGCG
                     YEREHEPSRRCKDEECPVCGSHLIRKNNLLAKDI"
     gene            complement(10661..11743)
                     /locus_tag="PKK03_08215"
     CDS             complement(10661..11743)
                     /locus_tag="PKK03_08215"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HNX84424.1"
                     /translation="MKITILLLMMMLTTLMSSCNMGENMFIASGYSRDGAADVLLCRL
                     NGNGSIDKISEIIVGDNPSYFTLGRGGLIYLVNEVDTFNTKAGGGITTLRYNKKSKSL
                     EKVSSMNQGGGGPCHIEVSVDGKFLITSNYGSGSVSVLRLNGEGIPEKVTDVIFYGEK
                     SHPHMTIHNPRLHTYYVSDLGLDRVHQLKLDTTLGLLMNADIAYFNGEPGSGPRHMAI
                     DKSSANLYVINELNSTATVYNILSDTVTAKQTVSALPEEFTEKSYCADIHLSRNGKKI
                     YGSNRGHNSIVTFRVGVDGKLSEPSHRDCGGNWPRNFAVSPSGKFFLVANQRSDEISV
                     VREGAHSDEAVSSLTLNAPSCVRFLK"
     gene            complement(11893..12141)
                     /locus_tag="PKK03_08220"
     CDS             complement(11893..12141)
                     /locus_tag="PKK03_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX84425.1"
                     /translation="MSVTPTTFLCTCGSRRLKPIIMAAMALLFSTLPMLFGSGLGNQL
                     QRLMALALIGGMTLGTFVSLCFIPLAYWYIYRRRGERV"
     gene            12258..12959
                     /locus_tag="PKK03_08225"
     CDS             12258..12959
                     /locus_tag="PKK03_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HNX84426.1"
                     /translation="MQPFKSNIVKQIETIPEGRIFTFADITFPRDKFANMAVILSQLT
                     KEQKLIRVERGAYYRPKQSSLGLGLLPVYQDEQLSYLTRKLGGYLTGAYVYNKMSLTQ
                     QVPSVITIAMRYPVRPFSFKKLSIECVKAYVDLPGDNETLHLIQILDAIKDLKYIPGV
                     SPQTAYERIFSLHVSVLSKSKLERIVSLAMVYPPRVRKILSDMLETGDNQDLREQLMA
                     MICPTTRFDLSYKIS"
     gene            12965..13963
                     /locus_tag="PKK03_08230"
     CDS             12965..13963
                     /locus_tag="PKK03_08230"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNX84427.1"
                     /translation="MNLHLNNEVFRELIELASDYFSYEQSHVEKDYWVCKILKELAIS
                     DFAGSVYFKGGTSLSKAYGIINRFSEDLDIFAFSGNVTPSKLSEKTLIRSISHFVIKN
                     NEDMYREELSVTGGDFRKLAFSYDIHYESAGLKENLEVEIKCCTLDDKSVMYYPAQKR
                     MVASIITEYLIMNERQDIIEQFALDAFEVTTIDPKRTLCDKISRLTRLSHSEDFEILI
                     AKYIRDVYDIYRILNTPEYIDFIRSEEFTVALTHVTHEDNLFRNSQLSNHVSEACIFA
                     RTEETLRLPAISRAYYDELRKLIFDSTKMPALEDVIKTMALLQEPLRRFDEKNYRI"
BASE COUNT         3600 a         3609 c         3214 g         3670 t
ORIGIN      
        1 ggcatctcag taacagatca ggtattgatt atcaaaatct tacttaagtt acgccaaaaa
       61 cagggcgatg tcaaatttat ttaggaaacc tcctgaaggc cgcccaatgg tcataccgga
      121 aaccaactcc cctaatcctc taccagccgg gcacaaaatt caacccgaaa actccgtttt
      181 taaaccctcc gttctgaaac gggaaggcat agtaaggctc aagaatgagg tatccgaaaa
      241 gatttatcct cagcgatgca ccggtactat acagtggaaa tttctcatta tctgccggat
      301 gagattcctg atcgttgaaa atgatcttgt cacccctgtt ccaagccatt cccgcatcga
      361 agaacaggtt aaggtcggtc agaaaccact ttgactttat gagggcaagc ctttcgggac
      421 cgctgaacgg gaaccttatt tctgcatttg ccaccgccat ccttgtgcca ttcaggcgcg
      481 agatattcag cgatgccagt cccggcgtgt ccccggtatt atacgaaata ttctcataac
      541 ctcttattaa ccagggataa ccgaggtata aaggctgcag gacattttcc tcggtcacct
      601 tcccgtacat gccgtaatta tacagccgga aggcaaaagt gaacggtttg cggtaaatgt
      661 actgcctgta gtctatgaga cctgtaaaga tttgcgtggc cccgaagtac ttgtcaacct
      721 ggaacctggc cctgcctccc tgcatcgggg cagtcatgcc gaacagtgag ttgtccttaa
      781 cataggcgaa cgaaagctgc tggtaactgc tgccttccgg agcaggcatc ttctccctgg
      841 ttccaccgat attgtaaccg ttcagcaaat agtaataatt gaacctgtca atgcgataat
      901 aataccatga tgccgaggct gttgcctcaa tccttctcac ctgggaaaag ggcagggaag
      961 catacagagt gatattatcc tcaaacatgc ggatatagtc aagctgaagc acatcaacag
     1021 gaaccatctc accctgcagc agaacggaat ctttaccgaa cgccatcgag ccggcacggt
     1081 aagggatatg agagacagcc gttccccact ttatcctgcc cttctgcctg acataggctg
     1141 cctgccctgc aaaatcatat atttcaccgt tcagcgacag ggatgaatag agctggttgt
     1201 caccgatcat atcactgaag atcatattga cactcccgcc caggttgttt ctgtaaatcc
     1261 ctgatgaaac tcccacactg gcattgttgg agatataatc gagcttgaat ctgggcttat
     1321 acggcaactc cttcacctcg tcagccggca actgtacaac ctcttttctg gcatacagcg
     1381 ccgagtctat gaggttaact gacaggtgct ccaaaggcgg gagggtgcct gcatcaaagt
     1441 tgaccactga tccatccgct tcttcagccc tgaattcatc tccatctgca accagaatat
     1501 gataagagtt tttaatgtag taattgtatg ccgtcttgtt cccgtcccgg gaccagctca
     1561 ttgccggtga ccacggcgta atcccgctta tgccggtcat gtacgaggta agcctcagga
     1621 gcctgccagt cgcaatttcg tatttgtaca tattcctgaa tccatcagcg tccgagagga
     1681 aataaatatg cgagtcatca gcagaaaaga cagggttcat attataggcg ccttcaaaga
     1741 catcgatctc gctgactttt cttgtactga gatcaagcat tgcgagggag taggaaaatt
     1801 tccggtgacc ggatgcattc attttttcct gtgagaaaac tattgtccgg ctgtcggatg
     1861 accatgatgg atgtatgttc gatgtaaaat catcggtcag tttttcaatg tcacctgaca
     1921 tgaggtcgta gaggtacagg tcacttatcc cttccgaaga tcctgtaaag acgatctttt
     1981 tactgtccgg ggaccatgag ggattggaaa ttgccggcac tcccctcatg gctacttccc
     2041 tggtgatcct gcctctcttt atgtcaacaa atgccagctt gttaattccg tcgctgaaca
     2101 ggacaaatac gtacttttta ctatcaggag accagcttcc gccggattcg acatagttga
     2161 aatcatcgta ggtacgggca gctgcaccag acaccttact gatgatcttc ccggttgaag
     2221 catcggcaac gaaaagatca agagtgaata tgtttttctc cgaaaagaat gcgacatact
     2281 ttccgtcagg actgatagca ggggagatat ttatcctgcc accattttca gtggttatca
     2341 gttgctttcc ggcaagctcg tcagcatcag ccttcaggta tggtctgtaa tagacctcca
     2401 tcgcactcgc ccaggcccct gaaagggttt tctcatccat tcccaggatg gtgtcagccg
     2461 cctttgataa tccatattgt gctgtcttcc tgaacagggg cataatggtt gtgtcacccc
     2521 acgttttccc caccattgcc cagaaagcct gaccatacct gtagggaaag tactttgagt
     2581 cagaggataa ctgcttaagc gtcgggaaat ccttattcaa aaccgcatcc ctcatccaca
     2641 ttgccgtatt gggatcaatg cttcccagcg agaagtattc tgccatccct tcaatcatcc
     2701 ataacgggat gttattcatt gaatatttct gagtggtgtc tgacctcagg aacatattga
     2761 actgaaatgc atgcacaagt tcatgaccca gggtgtggtc agtctgtgag agagtcagag
     2821 ctacaggcat gatgaccctg ttcttcatcc cctcagtaac ccctcccgta cctgttccga
     2881 taaggccact gacagcattg gtctgctgaa aatcagagtg attattgtaa aaaatcaggg
     2941 gattcttcct ttcaaaggta tccctgaaaa tcttctgatg aatgctgaac cactcctccg
     3001 accaccctga tatcgtcttc agtaatgaat cattcttcat gtaatgatag atctcgaaag
     3061 ccggcgtctg cagaacatcg aatctgaaag aactgtatcc cggtttgttc cttccgaaat
     3121 actgggcatc agaacttacc ccggtaatca gccatgctgt caatacagca aataacagcc
     3181 tgatgaccct gaccgatatt gtattctttt tgcacgaact catagcctgt caaagtttta
     3241 tcacctcaat actcctaaag ttaatccatg catgtcgatt aatcaaacta accgacctga
     3301 ataattgttc aaaaaatatg ccatttccca tggttggttt tgagtctgtt tatgaggtac
     3361 cgcagcatta aattttttat ttttgacaac cagcagcatt taatccggca acagaaaacc
     3421 cagacaatat gaaaaaacta tccatcatca cccttttgct tctcatggcc ctgatgggtg
     3481 catgcaaaac ggaaaagaca gaaaaggctg ctacgccggc catcgatgtc tcacttactg
     3541 acgccgaaaa agcaggaggt gtgcttaccc cggagatcat gtggaaatac ggccggatcg
     3601 ggacaattgc gctctcgccc gacggatcaa cagtactcta ccaggtgaca aactatgatc
     3661 tccccaccga ggcacgtgcc acaaacatct ggtccgtccc cgcatcggga ggcgatcccg
     3721 ttcagctgac caccgaggga ggcgcaggac cgcagtggat agagaacggc gccagaatcg
     3781 ccttcctcgc cggaggcaaa atcatggtca tgaatcctga cggcacggga aagaaagaga
     3841 tcaccgggct tgaggatttt gagatcctct cattctcacc ggccggagac aaggtttact
     3901 ttacccgtcg cgtcaagctc gaccagacgg caaacgagaa atacggactt ccaaatgcaa
     3961 aggtccgcat catcaatgac ctcatgtaca ggcattggga ctactggcat gactactcct
     4021 acagccatgt tttcgtcgcc tcatttgacg gtacggcaat tagcaacgaa aaggacatta
     4081 tggagggtca gcgttttgat gcccccgatg ccccgtggtt cgacgaggct gacctggcat
     4141 ggagccctga cgggaagtat cttgcatacg cctgcaagaa actgacgggc aaagcctatt
     4201 cgctcagcac caatacggac atatacctct acgacgtgac caccggcggg gagaagaaca
     4261 tcacagaggg aaacatggga tatgacaaga cgccggtgtt ctcccctgat ggttccatga
     4321 ttgcatactc cagcatggag gaagacggct atgagtctga cctcgagcgg ctgtttgttt
     4381 ataatattgc ttccggtgaa cggaaatggg tttcccaggg atgggactat aacgttggta
     4441 acatccagtg gaacggctcc gccgagatct ggttcaccag cgcctacctg ggcaggcagc
     4501 cggtattcaa aatttcactg gctgacggtg ctgtcacgaa ggtgaccgaa ggtgattacg
     4561 acatggggcc gctgcagctc aggaacaata cgctggttgc aggcattcag tcgatggcac
     4621 gcccgacgga ggttgcaaaa gttgatatgg ccggcggaca gttctcgatg atcaccgagg
     4681 taaacggtca tatttatgag agcataaaga tggggaagag cgaggagcgc tatatcaaga
     4741 cgaaggatgg caaggatctt cagatgtgga tcatctatcc gcctgacttt gacccctcga
     4801 agaaataccc ggcgctgctg tactgcaagg gcgggccgca gggaccgctc gggcagagct
     4861 ggagctaccg ctggaactac caggtgatgg ccgcaaacgg ctatatcgtg gtggctgtca
     4921 accgtcgcgg taactcgggc ttcggcagct actggcgtga gcagatctcg ggcgactacg
     4981 gcggcaagaa cattcaggat taccttgacg gtatcgatgc aatggcgaag gagcccttcg
     5041 tcgacaagga tcgtctgggt gccgtgggag caagctacgg cggctactcg gtcttctatc
     5101 tcgccggtat gcacaacaag aggttcaagg cgttcgtggc gcattgcggg gtttacaaca
     5161 ccgtagcaga gtacggctca accgaggagt actggtaccc gaacaaggat taccagggtg
     5221 cgccttggga caagccgcag cctccgggat acaacaagtt ctcacctcat acgatggtag
     5281 acaaatggga cacccccatc ctgatcatca cgggtgcgaa cgacttccgc ataccctaca
     5341 gccagtcgct tgaagcgttc aactcggctc agctccgtgg cgtgccggca cggctcctct
     5401 tcttcgagaa cgagagtcac tgggtcacca aaccacagaa ctccgttatc tggcagaagg
     5461 agtattttga gttcctggat agctatttaa agtaaaaaga ggcattaggc aataggcaat
     5521 aggcagtagt ggaggattac tgcctattgt tttttgtact gaatggtttg atgaccctct
     5581 gactacatga catcaggact gcaaaactct tagactttca ggttttctgt cacctccatg
     5641 acctgcccgg ccaggatttc aaatgcttcc acggctgtct tgtttgtctg ctggaagacg
     5701 gtgcgtccag cctcagcagc ctcgccaacc tccattacca gcggtatctg gcatagcaga
     5761 ttcgtatcat actccttcgc cagcttcgcg ccgccacccc tgccaaagag atagtaatgc
     5821 tcctcagggt gtgctgccgg ggtaaaccat gacatgttct ccactacacc aaggataggt
     5881 acctgcagat ctggattcat gaacatcgat gaggccttgc gggcgtcatt caatgctatc
     5941 tcctgcggag tggtaactat gattgcccct gagagattaa gcttctgtac cgtggtcagc
     6001 tgtatgtcac tggttccggg aggaaagtca actatcatat agtcaatttc accccattgt
     6061 gtatcctcaa aaagctgttt gacggcattg gcggccattg ggccgcgcca gatcagtgac
     6121 tggctgcgat ctatgaaaaa accaatggag atgatcttta ttccgaattt ctctaccgga
     6181 tacatgacat cctttcctcc ggccgattca acccttggcc tgacatcttc aaggccgaag
     6241 attgtaggta tcgatggacc gaagatgtca gcatccacca gtgctgtttt catgccgttt
     6301 cgcgccagcg ctattgccag gttggcagca accgtcgact tgcccacacc tcctttgccc
     6361 gatgctacaa ctattatgtt tttgataccc gccatgggta gcttgtcaaa caaattatta
     6421 ttttccattt gtgctcaaat tattccagat atcttttatt atgttgctta tctctgcatc
     6481 ggggaacatc tcggttacac tcctcccggc aaccatcgcc tcggttaccc tgctgtcata
     6541 gggtagtcgc cccagaagcg aagctccgtg ggcagtgcac catccctcaa tcctgtcagc
     6601 catggcatga ttgaggtcgc acttgttgat gatgatcccc acttttccgg taaacttcct
     6661 ggcaacttca aaaactcttt taaggtcttc aagtcctgac atggttggtt cagttacaat
     6721 aatcatcctg tcaacaccgg ttaccgtcga tatgaccggg cagccaattc ccggagggcc
     6781 atcgagcagg atgacaggca ctccctgttc cgtggcaacc ctctttgcct catctcttac
     6841 catactcaca agcttgccgg agttttcctc gccgggcgcc agtcgcccgt aaaccattat
     6901 accgtttctg aagctgccgg aatacatcct gcttttgtca ctgcgaacca tggtaatcgc
     6961 attcatcgga cagatacggg agcaaaaaaa gcatccgtcg catgatgtct ctgaaatcac
     7021 gatttttcca tttatctcct tgatggcatc aaaccggcaa taaccctcac aaattccgca
     7081 caacgtgcag agagtgtgat ccacaacggc cttatggccg ccgatataaa cctgctcatt
     7141 gttgtgttca ggattgaata tcaggtagag gttggccgca tctacatcac agtcggctac
     7201 caccagcctt tcacccacag tggcgaacgc tgcagtcacg cttgacttgc ccgtgcctcc
     7261 cttaccgctg attactgcca gttccatatc cttcaataat tttgtccgcc atatcctcaa
     7321 agagcgaagt cagcgccggg atctcatccg tcacaagttt gccctccgaa taaacccgtg
     7381 ctatggccgg atcatatttt atctctgcca gcaggggtat gttttctttg gcaaggtatt
     7441 cagcaacgct gccgtcacct atgtctgacc tgttgataat cacagccggc gtcttcttca
     7501 tctctctgag tgtttcaacg ctctgccgca ggtcactcag gccgaatggc gttggctcag
     7561 tcaccagcaa cacattgtca gccttgtcaa ctgtctgtat aaagggacaa gaggttccgg
     7621 gaggtgcatc gaggataacc actccatctt cacctgattc ctttatggct tccttgatta
     7681 tccttacagg cgacatctca ttgaccttca tccgggcttc aacaaccttt gagccgccat
     7741 cagtcataaa ttttgtaacc tttccaagca aaaccggttt ttccgttatt gctccggagt
     7801 tgcaggcaaa agtacatgcc ccgcacccgt ggcagagatt ctcaatgacc tctattacct
     7861 tcgctgccgg gacgtaaaat attgcattgt aattgcacca gcggctgcag cgaccacacc
     7921 atgtgcaggc atccttgttt atgaccggaa cctgatgggt cacttcagtc tcgctgacct
     7981 tttctgccct gaaaaagaga aggtcatccg gagcctcagc atcacagtcg gtcagggtca
     8041 ccgcttcgcc cctgcgtgaa agcacattaa agaggttagt ggcaacaaag gtcttgcccg
     8101 ttcctccctt gccactggct acagctatct tcatcgcgta tgcgggctaa tcataagcca
     8161 gcctcatctt ttcgtcggcc agcgcgggtt tgatgaaccg ctgcatggct tcacccttcc
     8221 aggtgccaag gttcatatgc gttgtgtaat acttctgcgg aaccggatgg gtgcctacta
     8281 ccacctcaat gccatatttc tcagggatga aacgggtgaa gtaatcaagc ctggggcacg
     8341 gtggataacc aaccaccagt cctgttgcca ggtgaatgac actcacaccg ttcttgatca
     8401 tctcctcagg ggtatattcc acgttgccgc cgggacagcc gccgcaggtg gtgaaggctg
     8461 ctatctcaac atcctcatcc atgtacctgt caaacgcacc ctcacggttg cggaatgacc
     8521 tgaagcactt gccacctgcg caggtatgat acctgtcgca tatgataatg ccaatccttt
     8581 tcttttccat gatgatatct taatggtcac agtaatttgc tccggcctga agcctctcat
     8641 tgagaaggtc tgcagcaagt tcatcaggac ttttcacagg agctcccaca aatacattta
     8701 ttgagtgctg gttaaagagg cctattgctc tctggcccat tcctccggca atcacatccg
     8761 tgatgccttt ctcagccagc cagccgggaa ggagtccggg ctcatgtggc ggcggagtga
     8821 tgagtgtttt ggcagtgatg gttttatcct cggcatcaac aattgcgaac tgctcgcaat
     8881 ggccgaaatg tgaacatagg ataccgcctt caagtggaat tgctatacgt ttcttcattg
     8941 ttcagtaatg attttttatg gtatatgaat cgattattta attaatagta agctatctgc
     9001 cggctttatc aacgctgaca accgcccggc tccgtttttt tcttcagcgg cctctgccgc
     9061 tccctttgcc acggcctgat ccggccccgc gaccggnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnggcc actgccctgg ccctgaacct ggccacttcc tcgtccgctg
     9241 ccctggccct gacctctgcc cttaccctgc ctgggtccct ggccgcgacc tctgccgcct
     9301 ggtgccactg aactctcttc cttatcagta gcattggcct cttccttgcc tgccttcccg
     9361 gcacggcccg caccgtaatt cgtgcactta ccggctttcc tgccggtcat cggtccctca
     9421 ccctgaggac ctttatgatc caatcctggc attttttcta tttattgctt attcaacata
     9481 tctatcaccg acttaacagt cttctccggc tccttgtaga ccaccatctg gatcctcaga
     9541 ctgtcaagaa gcgatttgat cttatagccg aactcgccgg agacgatctt ctcaacctcc
     9601 tttgatgcca ccagctggac tgaagccgga ccggctccct cctcggcatc cttgttgggg
     9661 tttgggagta tctccacccc gccggtctct gtatcgtaaa tgacaaaaaa ggcgcagcgg
     9721 ccaaaccgct ggtcgagctt gctctcaagg ctttgccccg tacttgttat tgctactttc
     9781 atatctgcta tttttaaata tcttttgcca gaaggttgtt ttttctaatg aggtggcttc
     9841 cacataccgg acactcctca tccttacagc gtcgtgaagg ctcatgctct ctctcataac
     9901 cgcagccggg acaaacgcag atgtcagaag gagcccggcg cgtaacctga gtcgagtcat
     9961 caacaggtac ataattgact atgctgccgc tgccgcataa cgggcacttt tcaggagtca
    10021 cactcttctc cggctggttg aagagacaac cgcagacgtt gcatttgtac cattcactgt
    10081 caaagtaaat cttaccacct tcgatgatga tctgcttacc tgttaccagt gcatcggcta
    10141 tctttgctct cgcttttgca taaatacggg taagcgtcgg acgcgagaca tccatcacac
    10201 gggcagcctc atgatggttg agattctcaa agtcacagag cctgatgact tcatactcct
    10261 cgaggtgcag gaagactgaa cggggctgac cgactttagc catcccgcca tagggtttga
    10321 acccggagat aaccggagga ttgctgactc ttcttaaacg tgttggacgc ggtgacactt
    10381 tttgatcatt tgttcgctac aaagataatg aacatatgat caaaacacaa aaacatcgtc
    10441 agctttctga taatttaaca ttaacaggac ataattgata cggattccag gcaggtatgc
    10501 ctggcgagta atccgtgaat ccgaatcgga atcagttcca aatgagttaa gattctttta
    10561 ctctgcggag aataagttca ttattctcat tgtttttgcg gcgattaact cgtacagctg
    10621 ccagcactgg ccgctctgat tttaacacaa acaatctttc ctacttcaaa aacctgacac
    10681 atgacggggc attcagggtc agtgaggaga cggcctcatc actgtgagcc ccttcacgaa
    10741 ccaccgaaat ctcatccgat ctctggttag ccaccaggaa gaatttgccg gatggtgaaa
    10801 ctgcgaagtt gcgcggccag ttgcctccgc agtcgcggtg agacggctct gaaagcttcc
    10861 catcaacccc gaccctgaag gtcacgatgc tgttatgtcc ccggtttgaa ccgtagattt
    10921 tcttcccgtt acgcgagagg tgtatgtcag cacaatagct tttctccgta aattcttcag
    10981 gaagggcgct gacagtctgt ttggcagtca cggtatctga aaggatattg tatactgtcg
    11041 ccgtggagtt gagctcattg atgacataca ggttagcaga tgacttgtca attgccatat
    11101 gccgcgggcc gcttcctggc tcaccgttga agtaagctat gtctgcattc ataagaagcc
    11161 cgagggttgt atcgagtttc agctgatgca ccctgtcgag gcccagatcg gagacatagt
    11221 atgtgtgaag tctcggatta tggatagtca tgtgggggtg cgacttctca ccgtaaaaga
    11281 tgacatcggt gaccttttca ggtatcccct cgccgttcag cctgagaacg gagacagatc
    11341 cgctgccgta gttggatgtg atcaggaatt tgccatcaac tgaaacctca atatggcatg
    11401 gtcctccccc tccctggttc atcgaactga ctttttcaag gcttttgctc ttcttattat
    11461 atcgtaatgt ggtgatacct cccccggctt tagtgttaaa agtgtcgact tcattgacaa
    11521 ggtaaatcag gccaccccta cctaacgtaa aatatgaagg attgtcccca acgattatct
    11581 ccgatatctt atcaatgctg ccgttgccgt tcagccggca tagcagcaca tcggcggcgc
    11641 catcgcgtga atagccggat gctatgaaca tgttctcccc catattacat gaagacatca
    11701 acgttgtaag catcatcatc agtaacagaa ttgtaatctt catcggattg cctgtatatc
    11761 attgtttacc tcagtctgtg tccgcagtcc gagcatggac ggatcaaaga caaatatgag
    11821 gtaatttttt taaatcttta ataaccgcca ggtaaagaat accataacct gtcagaaccc
    11881 cattcctcgc ggttatactc tctctccccg tctcctgtag atataccagt atgccagcgg
    11941 aatgaaacag aggctgacaa aggtgccgag ggtcatgcct cctataagcg ccaatgccat
    12001 cagccgctgc aactggttcc ccagacctga cccgaacagc atcggaagag tcgaaaacaa
    12061 caatgccata gcagccatga taattggctt cagcctcctc gaaccgcagg tacataagaa
    12121 tgtagtaggt gttacagaaa tgattttgag atggagattg atggcaacgt ttaggcgttg
    12181 gacttaacaa caaagataaa ttatgtttat acatgtaaat acataattaa tttcttatct
    12241 ttacaaaaaa aagagtaatg caaccgttta aatcaaatat tgtaaagcag atagagacaa
    12301 ttcccgaagg caggatattt accttcgctg acattacttt tcccagagat aaatttgcca
    12361 acatggcggt tatactttca caactaacaa aagaacaaaa actgattcgt gtggagagag
    12421 gggcgtatta caggccaaag caatcgagcc tgggattggg actattgcct gtttatcagg
    12481 atgaacagtt gagctatctg acacgaaagc tgggtggata tctgaccggg gcttatgtct
    12541 ataataaaat gtcattgacg caacaggttc catcggtcat tacgattgcc atgcgatatc
    12601 cggtacgacc tttcagtttt aaaaaactgt caatcgaatg tgttaaagcc tatgttgatt
    12661 taccaggtga taacgaaaca cttcatttga tacaaatatt ggacgcaata aaagatttga
    12721 aatatattcc gggtgtaagc ccccaaacag catacgagag aattttttcc ctgcatgtat
    12781 ccgttttgtc caagtcaaaa cttgaacgaa ttgtatcttt agcaatggtt tatcctccga
    12841 gggtcaggaa aatactcagc gatatgctgg aaacagggga taaccaggat ttgcgggaac
    12901 agcttatggc tatgatttgt ccgactacac ggttcgactt atcatataaa atatcatagt
    12961 gccgatgaat ttacatttga ataatgaagt tttcagggaa ctgatagaat tggcttccga
    13021 ttatttcagt tatgaacaat cgcatgtaga gaaagactac tgggtatgta agatattgaa
    13081 agagttggct atatctgatt ttgccggcag tgtctatttt aaaggtggaa cttcgctttc
    13141 aaaggcttat ggcataatta accgtttttc agaggatttg gacatctttg ccttctcggg
    13201 aaacgtcacc ccctccaagt tgtcagagaa aacactaatc cgtagtatat cccactttgt
    13261 cattaagaat aatgaagaca tgtacaggga ggaattatct gttacaggtg gagacttccg
    13321 aaagctggcc ttttcatacg atatacacta cgaaagtgcc ggactgaaag aaaaccttga
    13381 agtcgaaatc aaatgttgta cgttggacga taaatccgtc atgtattatc ccgcacaaaa
    13441 acggatggta gcttccatca tcaccgaata cctgataatg aacgaaaggc aagatattat
    13501 tgagcaattt gctcttgatg cctttgaagt gacaactatc gacccgaaac gtacactgtg
    13561 cgataaaatt tcacgattga cacgactatc tcatagtgaa gatttcgaaa tacttattgc
    13621 caagtatatc cgggatgtat atgatattta ccgaatcctg aatactcccg aatatatcga
    13681 tttcattcgc tctgaggagt tcacggttgc acttacacat gttacgcacg aggacaatct
    13741 atttaggaac tctcaattat ccaaccatgt ttccgaggca tgtatattcg cccgcactga
    13801 agaaacctta agacttcccg ctatttcacg agcctattac gatgagttga gaaagttgat
    13861 cttcgattcc actaaaatgc ctgcgttgga agacgttatc aaaacaatgg cattactgca
    13921 ggaaccgcta cgtagatttg atgaaaaaaa ctataggata tgagtttcaa acagtgtttg
    13981 gatttcagat catattccgg gaagggatag caatatttcc tttcatactt cgcagtccta
    14041 cagccatcca ttgacagcaa gctatcctaa agtatgagga accaacaaca tacttgatct
    14101 tgactcattg ttcattttgg gcatggcggt tttgtctgta gatataccag tacgccagcg
    14161 gaatgaaata gagactcaca aaggtgccaa ggg
//
LOCUS       DAOPXU010000071        13623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_4955_length_13623_cov_11.075103, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000071 DAOPXU010000000
VERSION     DAOPXU010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13623
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4955_length_13623_cov_11.075103"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..1648)
                     /gene="dinB"
                     /locus_tag="PKK03_08235"
     CDS             complement(209..1648)
                     /gene="dinB"
                     /locus_tag="PKK03_08235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNX84428.1"
                     /translation="MKKSLKVERSDVLRSCGFDSLTAGPSLPYGRDLSFAPADRQAAG
                     PHDGAQFQTFRPSDFQTFRPLTMDARSILHLDLDTFFVSVERVRDSRLNGRPVIIGGS
                     SDRGVVSSCSYEARSFGVSSAMPMKMARAMCPEAVFIRGDMEMYTKYSRMVTDIIADK
                     APLYEKASVDEHYIDLTGMERFHGCAGWSHELRDTIIRETGLPISIGLSVNKTVSKIA
                     AGEAKPNGEREVAQQQVLPFLDPLSIRKIPMIGQKTYHTLRSMGISTIHTLRVIPAEM
                     LGSLMGQNGMEIWKKANGIDNTPVISYSEQKSISTEHTFDRDTTDTVMLNQMIVSMTE
                     KLAYELRRQEKLASCLTVRIRYSDFDTHTLQQRVPYTAFDHVLISEAQSLFRRLWQRR
                     MLIRLIGVKVSGLVRGVQQLNMFEDTPEMVSLYMTMDRLRGRFGRHAIRRAAGVMTAA
                     ERSERELRKASELLAEKSRMEERLRGWRY"
     gene            1762..2559
                     /locus_tag="PKK03_08240"
     CDS             1762..2559
                     /locus_tag="PKK03_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HNX84429.1"
                     /translation="MRKRRGRTQDDVASALQMKRPTLSGYENGVAQPGIEALVAFSGY
                     FNISLDTMLKIDLTRLSESQLGELERGYDAYIRGSNLRVLTTTVAPDNRENIELVSEK
                     AKAGYTTGYADPEFIGDLPLFNLPFLSPDRKYRTFQLKGDSMLPIPDRSWVTGEFMQD
                     WRDIRSGQAYIILTSDDGIVFKVAENNIEKSGRLVLYSLNPLYEPYEVHISDIREVWK
                     FVHYISDELPEPVLPEKQLLRTVASMKQDLYQLKKSMGRDIEDAKEL"
     gene            2668..3999
                     /locus_tag="PKK03_08245"
     CDS             2668..3999
                     /locus_tag="PKK03_08245"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNX84430.1"
                     /translation="MKKTTILLISLLLSAAGDAFSQIPLSLKARHRTDGDTITLTSVL
                     RNRGLLSVRTSPDPVTGKSGNILLDDLRYYEIIPENLESLWQMIILRSPVYDNLLKSG
                     YSYETRSAMEDLMKEYLARAEQNNSFYIDSYLEARLYAILRKVYPVRNTDRRPGILSL
                     RILSEIAPDAWVGPDGTLVITTGLIAALDNETEMMALMAQEVAHFALDHHLSNYTSTV
                     TSGINPALGNIIRYTGQEERVADNCAISVLSYYGKNFSFLGSMLRNVMDYGELMGSFY
                     LGSFPNAKSRVDNYQGTITDKSADYDKLVAPVISYNAFMAYNQSHYLLCRRLLERNIA
                     SGTATADDIVLLSQAMMNLSGSDHEDLEALVLVRSVTDSLQEPPPGAFKQEALLLLRL
                     GRRDEAETALDRCREALEREEMKYSSKSGDWSEMLSYLASEKDWVARTRAR"
     gene            4140..4613
                     /locus_tag="PKK03_08250"
     CDS             4140..4613
                     /locus_tag="PKK03_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84431.1"
                     /translation="MPGKDYNPAMHTAEHILNSVMDKMYHCGRSFNSHIESKKSKCDY
                     HIRKGLSDKEVRAVERTVNEIIKLDIPVGEELVSRREADEKYFTGKLPGDAGETIRII
                     TIGNFDACPCIGQHVKNTSEIGTFSITSADFNEGILRIRFRIGQEVSQPGLRIPA"
     gene            complement(4699..5232)
                     /locus_tag="PKK03_08255"
     CDS             complement(4699..5232)
                     /locus_tag="PKK03_08255"
                     /inference="COORDINATES: protein motif:HMM:NF021231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HNX84432.1"
                     /translation="MKKLLFTVLLAAFTLPFAAAQGKLEVGKKAPEWALTDASKKEFT
                     MNSWPGKVLQINYVDPDNDDLNDPFNDAVNKAVDIDKIIDQNAFKGFGIVDLQSTKLP
                     DGLVRAIAGRKAKKYDTTILFDYKGLLHESWGMPKDSYTVVIVDKNRIVRGVYKGKIA
                     DSDIPGIIDMITKLTKE"
     gene            complement(5465..6130)
                     /locus_tag="PKK03_08260"
     CDS             complement(5465..6130)
                     /locus_tag="PKK03_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNX84433.1"
                     /translation="MISIDRSTLMFLSDLRRNNNRLWFAENRSRYEAARGNFSLFVQA
                     VIDDIVSFDPIFRGLEAKSCIYRINRDIRFTNDKTIYKTNFGAFMVRGGKKSADRFPG
                     YYLHIEPGQSFISGGSYIPPAPWLNSIRNRIAGEGDSLVKILNSREYRKYFGGLEGEK
                     LKLPPRGFSKDHPHIELIKMKSFLPEKLLTDREVVDPGFFEVVTGAFRAMKPLNDFLT
                     TGR"
     gene            6393..6938
                     /locus_tag="PKK03_08265"
     CDS             6393..6938
                     /locus_tag="PKK03_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84434.1"
                     /translation="MKISQLAALVILSAAMMISGCTREEEPDHFRYTVNDYPLYQGFI
                     HNYGMPEGASGYNFDVTIHSAGVSYNRDRHEFQGTGHLVFFQMFSSSATELAAGLYQF
                     SADDTPGDPSTFNVANFGMDINFSEDTGTLVSAVSGVVKVSGTGDYRTFDFDCLTGTG
                     EKITGHYYGFVPAYDMRDARK"
     gene            complement(7153..8298)
                     /locus_tag="PKK03_08270"
     CDS             complement(7153..8298)
                     /locus_tag="PKK03_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84435.1"
                     /translation="MKKITLFLLAVIISAGAMAQKGKITAAETFIREGAFDKAKEAIE
                     TAIVHPKSMGLAKTYYVKGKLCQAAFDSGDPKFIGLYPDILMEAYNNFEKAVQLDPKM
                     KNIIIRDNAYALLGNSFLNDAINKFNEKNFKGAMESFERNVKVSSSDLYVGMVDTLVI
                     FNAGLAAYNGEMYPEAIAYFRTCTESGTEDTKPYIFMSDCYMKMKDTVKAEAVLMEGF
                     KAYPDTLDIILQATQFYLDANNSPKAFEFVHKAMEMDPENHILYLVEGSLHMKLEDYP
                     KAIESLKTSLTKNPTQFESNYNIGLCYVSIANDMLNEANLISDNREYEIAKNKAFDEM
                     KKAIPFFLDAEKANPTSVATLEFLREIYLKLKMMPEFEAYKAKVENL"
     gene            complement(8372..10870)
                     /gene="gyrA"
                     /locus_tag="PKK03_08275"
     CDS             complement(8372..10870)
                     /gene="gyrA"
                     /locus_tag="PKK03_08275"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNX84436.1"
                     /translation="MSDQAKIVKVNIEEEMKSAYIDYSMSVIVSRALPDVRDGLKPVH
                     RRVLFGMSELGVLSNRPFKKSARIVGEVLGKYHPHGDSSVYDAMVRMAQDWSLRYPLV
                     DGQGNFGSIDGDSPAAMRYTEAKLKRIAEETLSDIEKETVDFQPNFDDTLQEPTVLPT
                     RIPTLLVNGASGIAVGMATNMPPHNLSEAVDAIIAYIDDPEITTEGLMHYIKGPDFPT
                     GGIIYGEQGIREAYETGRGRIVVRAKTEVELTHSGRECIVVTEIPYMVNKAEMIKRIA
                     DLINEKKLEGISYINDESDRTGMRVVIILKKEMPANIVLNHLYKHSQLQTSFNVNNIA
                     LVKGRPKTLALKEIIDSFVKHRHDIVVRRAKYDLDQAEKRAHILEGLIIASDNIDEVI
                     AIIRSSQNTDIARERLMERFGLTDIQARAIVEMRLRQLTGLEQDKLHAEYEEIKKLIE
                     YLNQVLADEKLQMQIIKDELLEVKDKFGDKRRTQIVPAAGEFNPEDFYADDEMVITIS
                     HMGYIKRTPLTEFRRQNRGGTGKRGSTTREEDFIEHMYVATMHNTMLFFTRNGKCHWQ
                     KVYDLPEGTRNSKGRAIQNVINIEPDDSVRAFINVKTIDDSDYINNNFIVLCTRKGVI
                     KKTSLEAYSRPRANGINAITVREGDELLEARMTNGSHEIMLAVRSGRAVRFKEATVRP
                     MGRTASGVRGITLSGDDDVVIGMITVENEAADVLVVSENGFGKRSAISDYRITNRGGK
                     GVKTINITDKTGKLIALKDVNDQYDLLIITQNGNILRTPVNELRVMGRATQGVKLIDI
                     KENDSIASVAYVEVNEDDNEPGIDIEEQSADSAE"
     gene            11114..>13623
                     /locus_tag="PKK03_08280"
     CDS             11114..>13623
                     /locus_tag="PKK03_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNX84437.1"
                     /translation="MDAHFSQRVNDILGYSKEEAIRLGNSHISPEHLFLGILREGEGV
                     AVDILLNNGVDLLMLKGSIEKSIKADRPVAMADNDILPLLKSTERVLRLVHIEAKAMK
                     TRIIDTEHLLMAILRDESNLVTRFLTELDIDYSLVKKAVEASNPAAQADYPRDEDDEG
                     IFSSPGSGKQQQGGQQAQQRSSSETPVLDNFGIDLTKAAEEGRLDPVVGREREIERLA
                     QILSRRKKNNPVLIGEPGVGKSAIAEGLALRITKRNVSRVLFNKRVVALDLASIVAGT
                     KYRGQFEERMKAILNELAKNDNIILFIDEIHTIVGAGGASGSLDAANMLKPALARGEI
                     QCIGATTLDEYRQHIEKDGALERRFQKVLVDPTTIEETIEILNNIKERYEEHHNVTYT
                     PEAIQACVRLTNRYISDRNLPDKAIDALDESGSRVHISNIVVPEKILALEKQLEETKK
                     EKSNAVKNQNYERAASFRDKERDILDLIEAEKKRWEKELALNREVVDEEKVAEVVAMM
                     TGVPVQRIAQTEGTRLLQMAEELKTSVVGQDEAIGRIVKSIQRNRAGLKDPNKPIGTF
                     IFLGPTGVGKTQLAKVLAKFLFDTTDNLIRVDMSEYMEKFSVSRLVGAPPGYVGYEEG
                     GQLTEKVRRKPYSVVLLDEIEKAHPDVFHILLQVLDEGQLTDSLGRRVDFRNTIVIMT
                     SNIGTRQLTEFGAGVGFDTSAKRTTRNEQNKSILQKALQKTFAPEFLNRIDDVIMFNQ
                     LTREDIDKIIDIELKGLFDRISALGYRIKLSPQARDFMADRGFDVQYGARPLKRAIQK
                     YLEDPLAEVIIKTAVAEGAVISVGYSKGKEELTFKVGG"
BASE COUNT         3437 a         3434 c         3329 g         3423 t
ORIGIN      
        1 ccacggtgag ggtgccgtaa cggaggctgt tgtatgagtg gcagttgatg tacattgtct
       61 catgtctgaa ggtctgatgt ctgaaggtct gaaggtctga tgtctgaagg tctgaaggtc
      121 tgaaggtctg tcgctttgtt ccgtctgaag gtcttgatat cttagaatct tttgactctc
      181 agactctcag attctcagac cttcagacct aatatctcca tccccgcagt cgttcctcca
      241 tgcgtgattt ctctgccagc agttccgacg cctttctgag ctcgcgctcg ctccgctccg
      301 cggcggtcat caccccggcc gcccggcgga tggcatgacg gccaaaacgg ccccgcagcc
      361 tgtccattgt catatagaga ctcaccatct ccggcgtgtc ttcaaacata ttcagttgct
      421 gcacaccccg caccagtccg ctcaccttca ccccgatgag cctgatgagc atgcgccgct
      481 gccagagacg ccggaataga ctctgcgcct cgctgatgag cacatggtca aaggcggtgt
      541 agggcacccg ctgctgcagc gtgtgagtgt caaagtcaga gtaccgtatc cgtaccgtca
      601 ggcatgacgc cagcttctcc tggcggcgca gctcataggc cagcttctcg gtcatgctca
      661 ctatcatctg gttgagcatc accgtgtcag tggtgtcacg gtcaaacgtg tgctcggtgc
      721 tgatagactt ctgctctgag taactgatga caggtgtatt gtcaatgcca ttggccttct
      781 tccatatctc catgccgttc tgacccatga gcgacccgag catctcggcc gggatgacac
      841 gcagcgtatg tatcgtggag atgcccatag agcgcagcgt gtgatatgtc ttctggccta
      901 tcatcggtat cttcctgatg gagagcggat cgaggaaggg gaggacctgc tgctgcgcca
      961 cctcccgctc accgttgggc ttcgcctctc cggcagcaat cttagagacg gtcttgttga
     1021 ccgacagccc gatggagatg ggcagccccg tctcccgtat gatagtgtca cgcagctcat
     1081 gcgaccagcc ggcacaaccg tggaatcgct ccatcccggt gagatcaatg tagtgctcat
     1141 ccaccgacgc cttctcatag agcggcgcct tgtcggcgat gatgtcagtc accatccttg
     1201 aatatttcgt gtacatctcc atgtcaccgc ggatgaatac cgcctcggga cacatggcgc
     1261 gggccatctt catcggcatg gccgagctga cgccgaagct gcgcgcctcg tagctgcagc
     1321 tcgagaccac cccgcggtca gagctgcctc cgatgatcac gggacggcca ttgagccggc
     1381 tgtcgcgaac cctctccacc gacacgaaaa aggtgtcgag gtcaagatgc aatatgctgc
     1441 gtgcgtccat ggttaaaggt ctgaaggtct gaaagtctga aggtcggaag gtctggaact
     1501 gcgctccgtc gtgcggtcct gcagcctggc ggtctgccgg agcgaagctg agatcccggc
     1561 cgtaaggcag agacggtcct gcagtcaaag aatcaaaacc gcaggacctt aagacatcag
     1621 acctttcgac tttcagactt tttttcattt tctgcttgtt atttcaaaaa cttaatttat
     1681 ctttgattag aaattaatca aacatgtgat tacaatataa tcaaattttg taatatgtac
     1741 ttcgcatcga acataaaatt tttgagaaaa cgccgcggac gtacacagga tgacgtggca
     1801 tcagccctgc agatgaaacg gccaaccctc agcggctatg agaacggcgt ggcacagccc
     1861 ggaatagaag cactggtggc tttttccgga tacttcaaca tctcgcttga cactatgctc
     1921 aagatagacc tgacccgcct ctccgagagc cagctgggcg agctcgagag gggatatgat
     1981 gcatatatca gggggagcaa cctcagggtc ctgaccacta cggtagcccc tgacaaccgg
     2041 gaaaatatcg agctcgtcag cgaaaaggcc aaggcgggct ataccaccgg ttatgctgat
     2101 ccggaattca ttggtgacct ccccctgttt aacctgccat tcctgtcgcc agaccgcaag
     2161 taccgcacat tccagctgaa gggcgactcc atgctgccca tccccgacag atcatgggtc
     2221 accggcgagt ttatgcagga ctggcgtgac ataaggagcg gacaggcata tatcattctt
     2281 acgtcggatg acggcattgt cttcaaggtt gccgagaata acattgagaa gagcggccgc
     2341 ctggtactct attcactcaa cccgctctat gaaccttatg aggtccacat aagtgacatc
     2401 cgggaagtat ggaagttcgt acattatata agcgacgaac tcccggaacc ggtactcccc
     2461 gagaaacaac tcctccggac cgttgcctca atgaaacagg acctgtacca gctaaaaaag
     2521 agcatgggtc gtgacatcga ggatgcgaaa gaactctgaa ccaatacgtt gctgcaaaca
     2581 aatatttttg tgccggcact gcaatgcagc acgatatttg tagtttattg tacattctga
     2641 aaccaatgtc atttaagagt tgttaccatg aaaaaaacaa ccattctcct gatctccctg
     2701 cttctttcag ccgcgggaga tgctttctcc cagatacctc tgagccttaa agccaggcat
     2761 cgtacagacg gagacacaat taccctgacc agcgtcctca gaaaccgcgg actcctttca
     2821 gtccggactt ctcctgaccc ggtaaccgga aagagcggga acattctcct tgacgacctc
     2881 aggtactatg agataattcc tgaaaacctg gaatcactgt ggcagatgat aatcctcaga
     2941 tcacctgtgt atgacaacct cctgaagtcg gggtacagct atgagacccg ctctgcgatg
     3001 gaggatctga tgaaggagta tctggccagg gcagaacaga acaattcatt ttatatcgac
     3061 agttacctgg aggccaggct atatgccatc ctgcgcaagg tctatccggt caggaacact
     3121 gacaggcgcc cgggaattct gagccttcgt atcctgtcgg agattgcccc tgacgcatgg
     3181 gtgggccctg atggcaccct ggtgattacc accggattga ttgcagccct ggacaatgag
     3241 accgagatga tggcactgat ggcccaggaa gtcgcccatt ttgcactgga ccatcacctg
     3301 tcaaattaca ccagtactgt cacatccggg ataaatcccg cactgggaaa tatcatcagg
     3361 tacaccgggc aagaggagag ggtggctgat aattgtgcaa tttcagtgct ctcatattat
     3421 ggcaagaatt tctcattttt gggttcaatg ctgcgaaatg taatggatta cggagagctg
     3481 atgggcagtt tttaccttgg atcattccca aatgccaaat ccagggtaga caactaccag
     3541 ggtacgatca cggataaatc cgctgattat gataaacttg ttgcacctgt tatatcctat
     3601 aatgccttca tggcatacaa ccagtcacat tacctgctct gtcgccgcct gctcgaacgg
     3661 aacattgctt caggcaccgc cactgccgat gatattgtgt tgctctcaca ggcaatgatg
     3721 aacctgtcag gttcggatca tgaagacctg gaggcactcg tcctggtccg ctcagtgaca
     3781 gacagcctgc aggagcctcc tcccggagca ttcaagcagg aagcccttct tttgctgagg
     3841 ctcggacgca gggatgaagc tgagacagcc ctcgacagat gccgggaggc actggaaagg
     3901 gaagaaatga aatacagttc aaagtctggt gactggtcgg agatgctcag ctacctggca
     3961 tcggaaaagg actgggtggc caggacaaga gcaaggtgac tccgctgaag cttcccggtt
     4021 tcagtgttct gcctgcaatc aacaatgatg atatttgcat cacagaccgg atatacctcg
     4081 gatcttgtta aaatattaat cccgctccaa cgtttcaata ttcaatatat acattcatca
     4141 tgccaggtaa agattataat cccgccatgc atactgccga gcatatcctg aactcggtga
     4201 tggacaagat gtaccattgc gggagatcat tcaacagcca cattgaaagc aaaaagagca
     4261 aatgcgacta ccatatcagg aaaggattgt ctgacaagga agttcgggca gtggaaagaa
     4321 cagtaaatga gataattaaa cttgacatac cggtgggaga ggagcttgtc agcaggaggg
     4381 aggctgacga aaagtatttt acagggaaac tgccgggaga cgcaggagaa acaatccgga
     4441 tcatcactat cgggaatttc gacgcatgcc cgtgcatcgg gcaacatgtg aaaaacacat
     4501 ctgaaattgg cacattttca ataacatcgg cagatttcaa tgaaggtatt ctgagaatcc
     4561 gcttcaggat cggccaggaa gtgtctcaac ccggcctccg gataccggcc tgatggttga
     4621 aaacaacgta aagcaaagct gacacacaaa aaaaaccggc gctataccgg tttttattta
     4681 tcatgattca ggtaattcct attcttttgt gagtttggtt atcatgtcaa tgatgcccgg
     4741 gatgtcgctg tcggcaatct tgcccttata tacacccctg acaatgcggt tcttgtcaac
     4801 aatcacaact gtatagctgt ccttgggcat accccatgac tcatgcagaa gtcctttata
     4861 atcaaacaga atggtagtgt catacttttt tgccttcctg ccggcaatgg cacgtaccag
     4921 tccgtcggga agcttggtcg actggaggtc gactatcccg aatcccttga aagcattctg
     4981 gtcaataatc ttgtcaatgt caacggcctt gttgacggcg tcattgaacg gatcattaag
     5041 gtcatcattg tcaggatcaa cgtagtttat ctgaagaact ttgcccggcc aggagttcat
     5101 ggtgaattcc tttttggaag catcggtcag agcccattcc ggagctttct ttccaacttc
     5161 gagcttgccc tgggctgcag caaacggcag agtaaaagct gccaggagaa cagtaaacaa
     5221 cagttttttc atttttacgg tttcttaaat ttagcggaaa gataacattt tttagtattc
     5281 atgcacatta tggcgaaaaa tgtgaagtga atccgggtga agcccatgat gttacctgcg
     5341 tgggctctgc agcactgtga atgtgaatgt tacctgggta aagcccatgc ttgaatcagg
     5401 aagagctttg cggcactctg acagccacaa tactgcgcgc cacccgcagg gattgacaaa
     5461 ctgattatct gcccgtggtg aggaagtcgt tcagtggttt cattgccctg aaagcgccag
     5521 tcaccacttc gaaaaaaccg ggatcaacca cctccctgtc tgtcagcagc ttttcaggca
     5581 ggaagctttt catctttatc agctcaatat gaggatgatc ctttgagaat ccgcggggcg
     5641 gaagctttag tttttctccc tctagtccgc caaaatactt cctgtactcc ctgctgttca
     5701 atatcttcac caggctgtcg ccttcaccgg caattctgtt gcggatcgaa ttcagccagg
     5761 gcgcaggagg gatgtatgac cctcctgaaa taaacgactg gcccggctcg atatgaaggt
     5821 aataacctgg aaacctgtcg gcgctcttct ttccgccacg gaccatgaac gccccgaaat
     5881 tagtcttata gatggtctta tcatttgtga accggatgtc tcggttgatc ctgtagatgc
     5941 agctttttgc ctcaagccct ctgaaaatcg ggtcaaaact gactatgtca tcaatgacag
     6001 cctgcacaaa cagggagaaa ttcccgcgag ccgcttcata gcgggaacgg ttttcggcaa
     6061 accagagcct gttattattt cggcgcaggt cagaaaggaa catcagggtt gaacggtcaa
     6121 tactgatcat atcattatca ggttaatcag ataaatctgt agaattgttt gttaaataat
     6181 tgaatatcaa tatgttacaa atgcacgtca gtttatccca aagctacgaa atttctctga
     6241 ttgccgtaac ggttcaataa ctgcatttct ctgattgaag taacggttta ataacatcat
     6301 tgttaagcca tttaaatcat agcatcagaa aacttttctg attggcagat gttaaacgca
     6361 tgaggcaatc tgtaatcaat aaaataaatg tcatgaaaat tagtcagtta gcagcattgg
     6421 taatcctgtc cgcagccatg atgatatccg gttgcaccag ggaggaagag cctgatcatt
     6481 tcaggtacac tgtgaatgat taccctctgt accagggatt tatccacaat tatggaatgc
     6541 ctgaaggcgc atccggttac aactttgatg tgaccataca ttctgcaggt gtaagttata
     6601 accgcgaccg gcacgagttc cagggtaccg gacacctggt ttttttccag atgttctcat
     6661 cgtcagcaac agaactggca gcaggtttgt accagttcag cgccgatgac accccgggtg
     6721 atccgtcgac tttcaacgtg gccaatttcg gaatggatat aaatttcagt gaggataccg
     6781 gaactcttgt atctgctgtc tccggagtgg tcaaggtatc agggaccggt gactacagaa
     6841 cctttgactt cgattgcctg acagggaccg gggaaaagat cacaggacac tattacggtt
     6901 ttgttcctgc ctatgacatg agggatgcca ggaaataaca tggttttggc aaacaaatgc
     6961 ctgacctcta tttgcctata attaatatta tgttaaatag aaacatcagt acaagggttt
     7021 caatagcaaa atcctgctga tggcgaaaag atggcccccc tgccctgtct ctggttgtga
     7081 gcgatgaagc gagccaaaaa aaaagaaggc acctcaacgg gtgccttcca tatttattct
     7141 ggtaacgctt tgttacaggt tctcaacctt tgccttatat gcctcaaatt caggcatcat
     7201 cttaagcttg aggtagatct cacgaaggaa ctcaagtgtc gcaactgaag tcgggtttgc
     7261 cttttctgca tccaggaaga aaggaattgc cttcttcatc tcatcaaatg ccttgttctt
     7321 ggcaatctca tactccctgt tgtcagagat caggttggct tcgttcagca tgtcatttgc
     7381 aatcgagacg tagcaaagac cgatattgta attgctctcg aactgagtcg ggttcttggt
     7441 gagtgatgtc ttcaggctct cgatggcttt cgggtaatct tcaagcttca tgtggaggct
     7501 tccctcgaca agataaagga tatggttttc aggatccatc tccatggcct tgtgaacaaa
     7561 ctcaaatgcc ttcggcgagt tgttggcgtc aagatagaac tgggttgcct ggagaataat
     7621 atccagggtg tcaggatatg ccttgaaccc ttccatcaga acagcttcgg ccttgacggt
     7681 atccttcatc ttcatgtagc agtcgctcat gaaaatataa ggtttcgtat cctcggtgcc
     7741 cgactcggtg caggtacgga aatacgcaat tgcttcagga tacatctcac cgttgtaagc
     7801 tgccagcccg gcattgaaga tcaccagtgt gtctaccatg cctacataga gatcgctgga
     7861 ggaaaccttc acgttccttt caaatgattc catagctccc ttgaagttct tttcattgaa
     7921 cttgttgatt gcgtcattca ggaatgagtt gccaagaagt gcatacgcat tgtccctgat
     7981 gatgatgttc ttcatcttgg gatcaagctg aactgctttc tcgaagttgt tgtaggcttc
     8041 catgaggatg tcaggataga gaccgataaa cttcggatcg cctgaatcaa acgcagcctg
     8101 gcaaagtttt cccttaacgt agtaagtctt tgcaaggccc attgacttcg ggtgaacgat
     8161 ggctgtctca attgcttcct tagccttgtc gaatgcacct tccctgataa aagtttcggc
     8221 agcggtaatt tttcccttct gtgccatggc acctgctgag atgattactg ccagtaaaaa
     8281 caatgtgatc ttcttcatgt ctggataatt tgcttttaat gttggtccaa aaataaaact
     8341 ttagaatcta ttcttcaaat aatttgccaa attactcggc actgtcggcc gactgctcct
     8401 caatatcgat accaggctcg ttatcatcct catttacttc gacataagct actgaagcaa
     8461 ttgagtcatt ttctttgata tctatgagtt ttacgccctg tgttgccctt cccatcactc
     8521 tcagttcgtt caccggcgtc ctcaggatgt taccgttctg agtaataatc agcaggtcat
     8581 actggtcgtt cacatccttg agggctatca gtttcccggt cttgtcagtg atgtttatgg
     8641 tcttcactcc ttttcctccg cggtttgtga tacggtaatc gcttatggcc gacctctttc
     8701 cgaagccgtt ttccgagacc accagcacat ctgccgcttc gttctcaacg gttatcattc
     8761 cgattacaac atcgtcatcg ccggagaggg ttattccgcg tacacctgat gccgtacggc
     8821 ccattggcct gacagtggct tctttgaatc tgacggcacg gcctgatctc actgcaagca
     8881 taatctcatg gctgccgttg gtcatcctgg cttcgaggag ctcatcccct tcccttaccg
     8941 tgatggcgtt gatcccgttc gccctggggc gggaataggc ttcgagcgac gtcttcttga
     9001 ttactccctt gcgggtgcag aggacgatga agttgttgtt gatataatca ctgtcatcaa
     9061 ttgtctttac attgatgaat gccctgacgc tgtcatcagg ctcaatattg atcacgttct
     9121 gaatcgccct ccccttactg ttgcgcgtac cctcgggcag gtcatagacc ttctgccagt
     9181 ggcacttgcc gttacgggta aagaagagca tggtgttatg cattgttgca acatacatat
     9241 gctcaatgaa atcttcttcc cgggtagtgc ttcccctctt gccagtacct cccctgttct
     9301 ggcggcggaa ctcggtcaga ggcgtccgct tgatgtaacc catatgcgaa atggtaataa
     9361 ccatctcatc atcagcgtag aaatcctcag gattgaattc gcctgcggcg ggaacaatct
     9421 gagtccgccg tttgtctccg aatttgtcct ttacctcgag aagctcatcc ttgataatct
     9481 gcatctggag cttctcgtct gcaagaacct ggttcaggta ttcgatgagt tttttaatct
     9541 cctcgtattc agcatgcagc ttatcctgtt ccagcccggt gagctgcctc aggcgcatct
     9601 ccacgattgc tctcgcctga atatcagtca gcccgaatct ctccatcagc cgctcacgcg
     9661 cgatgtcagt attctgcgaa gacctgataa tagctatgac ctcatcgata ttatcgctgg
     9721 caatgatcag accttcaagt atatgagctc tcttttcggc ctggtcaaga tcatatttcg
     9781 ccctgcgcac aacgatatca tgcctgtgct tcacgaagct gtcaattatc tccttgaggg
     9841 caagggtctt gggccgaccc ttgacaagtg ctatattgtt gacattgaac gatgtctgca
     9901 gctgcgaatg cttgtaaaga tggttcagga caatgttggc cggcatctcc ttcttgagga
     9961 ttatcacaac cctcattcct gttctgtcag actcatcatt gatataggaa atcccttcca
    10021 gctttttttc attgataagg tcagctattc tcttgatcat ctcggccttg ttgaccatat
    10081 aggggatttc ggtgaccacg atgcactctc ttcccgagtg ggtcagctcc acctccgttt
    10141 tggccctgac aacaatcctt cccctgccgg tttcataagc ctcccttatc ccctgctcac
    10201 catatattat acccccagtc ggaaagtcgg gtcccttgat ataatgcatc agcccctcgg
    10261 tggtgatctc cgggtcatca atgtaagcta tgatggcatc aactgcttcg gagaggttgt
    10321 gaggaggcat gttggttgcc atacccactg caatacctga agctccgttg accagcagcg
    10381 ttggtatcct ggtgggcaat acggtaggct cctgcagcgt gtcatcaaaa ttgggctgaa
    10441 agtcaacagt ttccttttca atgtccgaga gagtctcttc agcgatccgc ttgagctttg
    10501 cctctgtgta tcgcattgct gcaggactgt caccgtctat tgatccgaag tttccctgcc
    10561 cgtcaacaag cgggtaccgg agagaccagt cctgcgccat ccttaccatt gcatcataaa
    10621 ccgatgagtc accatgcggg tgatacttac ccagaacctc ccccactatc cttgctgatt
    10681 tcttgaatgg cctgtttgaa agcactccaa gctctgacat accgaagaga accctcctgt
    10741 gcaccggctt taaaccgtca cgcacatcgg gtaatgccct tgacactatg accgacatcg
    10801 aataatcgat gtaagccgac ttcatctcct cctcgatatt gacttttact atttttgctt
    10861 gatctgacat atcagattga taattaataa gttacaaata tatttttctc atttgaaatt
    10921 aatccacgaa aataatcaaa atgcctcatt ttttaacctt ttgaagcctc tatttgttaa
    10981 cagttgatgt taaaaattgg gcacaattct tgccaataca tttttacaat agaattaact
    11041 ttgaaatctg gcgttttatg tattaatggc agttttactg acacaattgc atgaaacccg
    11101 taacgaaaaa ataatggacg cacatttctc acagagagtc aatgatatcc ttggttacag
    11161 caaagaagag gcaatcaggc ttggcaacag ccacataagc ccggaacacc tcttccttgg
    11221 catactccgc gagggcgaag gagttgcggt tgatatactg ctgaacaacg gagtagacct
    11281 gctcatgctc aaaggatcga tagaaaagag catcaaggcc gacaggccgg tggcgatggc
    11341 agacaatgac atactgccac tgctgaaaag cactgaacgg gtcctcaggc ttgtacatat
    11401 tgaagccaaa gccatgaaga ccaggattat agataccgaa caccttctga tggccatcct
    11461 ccgcgatgag agtaaccttg tcacccggtt cctgacagaa ctagacatag attattcgct
    11521 tgtcaaaaag gcagtcgaag ccagcaatcc tgccgctcag gcagattacc cccgtgatga
    11581 agacgatgaa gggatctttt ccagtcccgg ctccggaaaa cagcagcagg gcggacagca
    11641 ggcacagcag aggagctcat ccgagactcc ggtgcttgac aacttcggca tcgatcttac
    11701 caaggccgca gaggaaggga ggcttgaccc ggtggtcggc cgtgaacgcg agattgagcg
    11761 tctggcgcag atactctcaa gacgcaagaa gaacaacccg gtgctgattg gggaacccgg
    11821 agtaggaaaa tcagcaattg cagaaggact ggcgctgagg atcacaaaga ggaacgtctc
    11881 gagggtgctc ttcaacaaga gagttgtggc gctcgacctg gcatcaatcg ttgccggcac
    11941 caagtaccgc ggacagtttg aggagagaat gaaagccatc ctcaatgagc tggctaaaaa
    12001 tgacaatata attctcttta ttgatgagat acacaccatt gtcggggcag gtggagccag
    12061 cggctcactt gatgctgcca acatgctcaa gccggcactg gcaaggggtg agattcagtg
    12121 cattggcgcc acaacccttg atgagtaccg tcagcatatc gagaaggacg gcgccctgga
    12181 acgccgtttc cagaaggtgc tcgttgaccc gacaacaatc gaagagacaa tagaaatcct
    12241 caataatata aaggaacggt acgaggagca ccataacgtc acctataccc cggaggcaat
    12301 ccaggcatgt gtccgcctca ccaacaggta catcagcgac aggaacctgc ctgacaaggc
    12361 aatagacgca cttgatgaat ccggctcaag ggtgcatatt tccaatattg ttgtgccgga
    12421 gaagatactg gccctcgaga aacagttgga ggagaccaag aaggaaaaga gcaacgccgt
    12481 caagaaccag aactacgaac gcgccgccag cttccgcgat aaggaacgtg acatccttga
    12541 cctcatagag gcagagaaga aacggtggga gaaggaactt gcgctgaacc gcgaagtggt
    12601 tgacgaagaa aaggttgccg aggtggttgc catgatgacc ggcgtgcccg tgcagaggat
    12661 agcacagacg gaagggacac gcctgcttca gatggcagag gagctgaaga ccagcgtcgt
    12721 gggccaggat gaagccattg ggaggattgt caaatcaatc cagcgcaaca gagccggact
    12781 caaggaccct aacaagccga tcggcacgtt tatattcctc ggtcccaccg gggtgggaaa
    12841 gacacagctt gccaaggtgc ttgcaaagtt tctctttgat accactgaca acctgattag
    12901 ggttgacatg agcgagtata tggagaagtt ttcggtgtca cgccttgtcg gggcgcctcc
    12961 cggatatgta ggttacgaag agggcggtca gctgaccgaa aaggtgaggc gcaagcctta
    13021 ctcggttgtg ctgctcgatg agatagagaa ggcgcacccg gatgtgttcc acatcctgct
    13081 gcaggtgctg gacgaaggcc agcttactga cagcctcgga cgaagggtcg atttccgcaa
    13141 caccatcgtg ataatgacct cgaacatagg cacccgccag cttaccgagt ttggcgccgg
    13201 tgtcgggttt gacacctcgg caaagcggac gacgcgcaac gagcagaaca agagtatcct
    13261 gcagaaagcc ctgcagaaga ctttcgctcc cgagtttctg aacaggattg acgacgtcat
    13321 catgttcaac cagctcacca gggaggatat cgacaagatc atcgatatag agctcaaggg
    13381 actgttcgac aggatatcgg cgctgggata caggataaag ctgagtccgc aggctcgcga
    13441 tttcatggca gaccgtggtt tcgacgtaca gtatggtgcc aggccgctga aacgggctat
    13501 tcagaagtac cttgaggatc ctctggctga ggtcattatc aaaaccgctg tggctgaggg
    13561 tgctgttatc agcgtcgggt attcaaaggg taaggaggag ctgaccttca aggttggcgg
    13621 caa
//
LOCUS       DAOPXU010000072        13559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_4989_length_13559_cov_11.922764, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000072 DAOPXU010000000
VERSION     DAOPXU010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13559
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4989_length_13559_cov_11.922764"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..1557
                     /locus_tag="PKK03_08285"
     CDS             112..1557
                     /locus_tag="PKK03_08285"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX84438.1"
                     /translation="MKTILYKILVLSVIAGAASAELRSQAFPDSLAAYLEIAARNNPG
                     VQKSLYDYQAALQKVPQVSSLPDPELTAGFFLKPMELMSGNQVADFQLMQMFPWFGVL
                     RNAKDEMSLMAQAKYELFRDAKLQVGYDVQKAWYELFKVREEIRISEKNIELLRTIER
                     LSLIKFKSPSAGGTTSLPAGSMTSPGSRGGMEGGSAGMGNMAGVQTTRRDADSRENQS
                     SMQSLPMGSSTSGSGLADFYRIQIEILGLEDNIASLKNQERTSVARFNSILDRSPEHP
                     VYVDTITRYSSVGLEETMDPDSLLADNPMLKMISYENQSLEARKKMISGMSYPMMGIG
                     INYSLINKSPMSESAMNGSDMVMPMVTVTIPVYRKKYNAMKSETDLLKSSSEKSYRAT
                     ENSLKVELLSAMLDYQDISRRVKLYESQHSLAARSLDLMLKDFSASASSLTDVLRLRQ
                     QIYEYELNLIEARADLNITSARITRLMAYTI"
     gene            1575..3233
                     /locus_tag="PKK03_08290"
     CDS             1575..3233
                     /locus_tag="PKK03_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX84439.1"
                     /translation="MEKLNNSQLNRLFSNRYFNYTLFVLAGLFLGWLIFRDGHGHDHE
                     PRQSTEVAQETIWTCSMHPQIRMQEQGDCPICGMDLIPLAQNGSSGIDPDAVHLTKEG
                     AALANVMTTVASRQKPVMEVRLYGKVQADERLLQNQVSHVSGRIEKLFVNFTGESVSK
                     GQLLAQVYSPELVSAQQELIEAAKTKETQPAIYDAAREKLRLWKITDAQIETIENSGI
                     AQSLVDIVSNTDGTVISRLVNTGNYISQGTLLYEIADLSGLWVLFDAYESDLRFLKRG
                     ERLTFTVEAYPGEKFTGTIAFIDPVIDPVTRVARVRVETGNRSGRLKPEMFVTGIFST
                     ALPEFSNNLIIPKSAVLWTGKRSIVYVRQPGIDEPVFTMREVELGPALGDSYVVVSGL
                     AEGEEIVTNGTFSVDAAAQLAGKPSMMNPVGGASSTGHDHGTMSETGSVEGNTTESDH
                     DHSTTATTGDNLSASTGHTEFRVEGNCEMCKERIETTAKKVHGVETANWIIDTKQLHL
                     TFDKSHTDLDEVKKAIADAGHDNDKYRAPDTIYNSLPECCLYRK"
     gene            3253..4080
                     /gene="larE"
                     /locus_tag="PKK03_08295"
     CDS             3253..4080
                     /gene="larE"
                     /locus_tag="PKK03_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HNX84440.1"
                     /translation="MEQKITNLIDLLRQHKSAVIAFSGGVDSTFLARIARDAYGDNLL
                     LITATSSTYPYYELDEARSLAELLGIKHRIIVSEEIEIPGYADNPPDRCYYCKSELFG
                     KIKFIAEQEGYEAVFDGSNADDLKDYRPGMRAKQEKGIISPLAETGFTKNDIRHFSAL
                     MNLPTATKQSYACLASRFPYGEKITIEKLDRVGAAEFEIRKMEFTQFRIRSHENLARL
                     EFIPSEMDKAWNNRDHLSLICKRSGFSYTTIDLTGYRTGSMNEVLPESVRNERNENH"
     gene            4107..5177
                     /gene="moeB"
                     /locus_tag="PKK03_08300"
     CDS             4107..5177
                     /gene="moeB"
                     /locus_tag="PKK03_08300"
                     /inference="COORDINATES: protein motif:HMM:NF004281.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNX84441.1"
                     /translation="MTSSRNLTREEKARYSRQIRLAEIGEEGQLKLKASSVLVIGAGA
                     LGCIVLQYLTAAGIGTIGIVDNDWIDVSNLHRQVLYDISDISKPKILASRARLERLNP
                     EVKFNTYFLRLNKDNAADIIKEYGIIIDCTDNFATRYLINDVCVLLNKPLVYGAILQF
                     SGQVMVLNYRNGPTLRCLFPDPPHPLEVPSCAEAGVIGSIAGIIGSIQATEVIKMIVG
                     AGEILSGKMLILDSLNYSTEIIAFERDPESSVVHSLGEYEDACLSEDDSVNEISAEDL
                     RKMLSVNPGTKVIDLRDKSDPENPEFESIRIPYYEISRRPDIFTGSEPVVFWCRYGIK
                     SRQVINYLKKYHGRNNLYSLVI"
     gene            5143..6213
                     /gene="hydE"
                     /locus_tag="PKK03_08305"
     CDS             5143..6213
                     /gene="hydE"
                     /locus_tag="PKK03_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HNX84442.1"
                     /translation="MDETTYTALSSDRNQIISLLASTGDLRKSLFEHAARVKREHVGD
                     LVYFRGLIEFSNNCRKNCLYCGIRSGNRKVKRFNLFDEEIVSAAIFANDNGYGSIVLQ
                     SGEIHGKMFTKRIESLLKKIHAATNDRLRITLSCGEQEKDVYRRWFESGAKRYLMRIE
                     TSNQDLYARLHPNDQRHSFRKRLDCLNILKETGYQTGTGVMIGLPFQTPEDLADDLLF
                     LKNNDIVMIGMGPYLEHADTPLYRYRSQLLPVNERFDLTLKMIAILRIMMKDVNIAAT
                     TALQTIDKLGREKAIMAGANVIMPNITPGMYRNDYSLYDNKPCTDENPEDCRTCLEAR
                     IALTGNNIAFGEWGDSAHFPGL"
     gene            6309..6740
                     /locus_tag="PKK03_08310"
     CDS             6309..6740
                     /locus_tag="PKK03_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84443.1"
                     /translation="MIKRITVILIFMLAGILLVAHAVVPHHHHDKQVCFEKSHCIHED
                     SDEEPGTSHDGHSHDGENNHNDCALNDPVVITTNEWKPDFKLIQASDRTGFDGLHVSL
                     LNNSSGCLTPSLSSCVYERVTDSLFPSAVPDSHGLRAPPAA"
     gene            6833..>7832
                     /locus_tag="PKK03_08315"
     CDS             6833..>7832
                     /locus_tag="PKK03_08315"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX84444.1"
                     /translation="MYFKLLLTSLIVITVAGCNHSHEKGSDTESEEIKFQYTAYSDQY
                     ELFAEADPFIAGQTANVLSHLSNLPEFTAVTSGEVTIKLIVNGKSVEQTLESPSRKGI
                     YSFDVRPDVTGTGTLEYIVKTETDDFKISVPEVVVYAGNQEAATAAAMNKTARTNTTV
                     FTKEQSWKTEFSTGYPEREPFGQVIKTTAQIQSAQGDEALVTAKTSGVISLQGNSLTE
                     GMNVSNGQALFMIKGSGLAENNSTVRFAEAQNNFEKASADYERARLLAEDKIVSEKQL
                     LETKTIYENARSVYENLKQNFSAAGQTVFSPMSGFVKQLLVRNGQYVGAGEPLTVIS"
     assembly_gap    7833..7932
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7933..8490
                     /locus_tag="PKK03_08320"
     CDS             <7933..8490
                     /locus_tag="PKK03_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNX84445.1"
                     /translation="LVRNGQYVGAGEPLTVISQNKSLLLRAEVQQKYARDLMSITAAN
                     IRTIEDNKTYTLEELNGKVLSVGRTTTTDNFLIPFTLQINNAGSLVPGGFVELYLKTV
                     SNSKALTIPVSAVLEEQGNFFVYVQVTPELFEKREIKTGATDGIRTEVISGLVDDERI
                     VLKGAVLIKLAQATGSLDAHSGHVH"
     gene            8505..11606
                     /locus_tag="PKK03_08325"
     CDS             8505..11606
                     /locus_tag="PKK03_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX84446.1"
                     /translation="MINNIIKFSLNNKYLIILLSVVLVVFGMRTALNMDVDVFPDLTA
                     PTVVVMTDAHGMASEEVERMVTFPIETSVNGATGVRRVRSASSQGFSFVWVEFDWGTD
                     IFRARQIVSEKLITISSRMPLGVGQPVLAPQSSVMGEIFFIGVQADSTSLMDLRTIAE
                     WNIKPLLLATGGVSQVTNIGGDYKQYQVLANPQKMKFYRVSMTELAEVCKGLSQNSTG
                     GVVRQYGNEYVVRGIARTSDLTTLGSSYVKSVNGKPVRLNDVAEIKIGSAVKMGYASE
                     NANPAIIISVSKQPNTNTLNVTERIEENLRTLQKTLPADVKLDTKIFRQADFIETSVS
                     NVQKALLEGAVFVVLILFLFLGSFRTTIISLIAIPLSLLGAILVLRLLGLNINTMSLG
                     GMAIAIGALVDDAIIDVENVYKRLRQNRQKPVELRQDAFTVVFEASKEIRASILNATL
                     IIIVAFIPLFFLSGMEGRMLKPLGISFIVSLFVSMVVAMTLTPLMSKMLLSNDRYLAR
                     NEKEKWLVRKLSYYYEESLRWSLNHKRSIVLSTLGLFFAAIIAMSTMGRSFLPEFNEG
                     SLTLSVITKPGTSLEESNNLGNLVETELLSIPEVSSTARRTGRGELDEHSQTTNSAEI
                     DVNFDLNERSREEFMADVRRTLSGIPGIAFTVGQPLGHRIDHMLSGTRANIAVKLFGS
                     DLNKMFSIGNEIKSSIADVEGLVDVNVDQQIEIPQIQIRANRDMLAQYGITTNEFNEF
                     VDIAFGGEKLADIYEGQRSFGLVLRMDTDYLENMEGIRSALIDTHDGRKVPLEQVADI
                     VSVTGPSSISRENVQRKIVVSANVAGRDLRGAVQDIQKNINESVVLPEGYRIEYGGQF
                     ESEAKASQTLMLTSLLALVIIFLLLFQEFKNFKLAGIILLNLPLALIGGVFAIYFTSG
                     ILSIPAIIGFITLFGIATRNGILLVSHYQHLQASGTSLTDTVTKGSADRLNPILMTAL
                     TAALALIPLALQGNLAGNEIQSPMAKVILGGLITSTLLNIYIVPIVYSALRTRGILKN
                     DNL"
     gene            11603..12778
                     /locus_tag="PKK03_08330"
     CDS             11603..12778
                     /locus_tag="PKK03_08330"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX84447.1"
                     /translation="MKRLIIVLSTVILGSANLLSQDIDRILAGVEKNNTTLVALRKSA
                     DAELISNKTGLYPENPEFAFNYLWGSPESIGNRKDISMMQSFDFPSAYAYRNQISEQK
                     NVQVELEYERQLKSIMTQTRLVCNELIYHNALAIELKQRIVNAQKIADSYKSKFEAGE
                     TGIIDFNKAQVYLLNIIKDAESCDIERSSHLAELKSLNGGLPVDFNDSIFPLQTLPVD
                     FEQWYADAEKNNPVLRWLNQEISISQKNEKLNAAMGLPKIQAGYMSEDVVGEQFRGVA
                     LGLTLPLFENKNAVKYAKAKTFAVQSAETDSRQQFYNNLKALHTKAGSLQNSLNDYRS
                     NLQKFNNSALLQKALDNGELSLAEYYLELTVYYESFNRLLDIEKSLNETVIELFRYQ"
     gene            12790..13437
                     /locus_tag="PKK03_08335"
     CDS             12790..13437
                     /locus_tag="PKK03_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNX84448.1"
                     /translation="MKENEQIFYKRAFWLSLFTIFYNIIEGVVSMALGYADETLSLFG
                     FGADSFIEVLSGIGIAIMITRIRQNPDSPKSRFEITALKITGTAFYLLSAGLFAGIIL
                     NIIYKHKPETTIWGIVISLISIAVMIWLMNAKKSIGKKLHSEPIISDANCTRVCVYMS
                     VVLLVSSLVYEFTGFAYADAIGAAGLIYFSVSEGREAFEKAKGKSCSCGEHCEAD"
BASE COUNT         3959 a         2944 c         3230 g         3326 t
ORIGIN      
        1 tacaggttat gacaattttt gttgttccgg ttttccaggc aatctggagg gaaggcgaag
       61 tgtcacggtc tgaaaagtta caagatcagt taccaaacaa agaaacggaa gatgaaaaca
      121 atactttata agatccttgt tttgtcagtc atagcgggtg cagcgtcagc agaattaaga
      181 agccaggctt tccctgattc cctcgctgct taccttgaaa ttgccgccag gaataaccct
      241 ggagttcaaa agtcgctgta cgattaccag gctgcacttc aaaaggtacc gcaggtaagc
      301 agcctgcccg accctgagct gactgcaggc ttcttcctta aacctatgga gcttatgagc
      361 ggaaaccagg tggctgactt tcagcttatg cagatgttcc cctggttcgg agttttgcgc
      421 aatgcgaaag acgaaatgag cctgatggca caggctaaat atgagctgtt ccgcgatgcg
      481 aaactacagg ttggttatga cgttcagaaa gcctggtatg agctgttcaa agtccgtgaa
      541 gagattagga tttctgaaaa gaatattgaa ctactgagga ctattgaacg cctttcgctc
      601 attaaattca aatctccttc tgcaggaggc acaacgtcac tgccagccgg ctccatgaca
      661 tcccccggct cccgtggagg gatggaagga ggttctgcag gaatggggaa tatggcaggt
      721 gttcagacaa ccaggaggga tgcagattca cgggaaaacc aatcctcaat gcagtcactc
      781 ccaatgggtt cttccaccag cggatcaggt ctcgcagatt tctacaggat acagatcgag
      841 atcctcggac tcgaggacaa cattgcctcc cttaagaacc aggaaagaac atctgttgcc
      901 aggttcaaca gtattcttga caggagtcct gaacatccgg tttatgttga cacaatcaca
      961 cgatactctt ctgtgggatt agaggagaca atggacccgg acagcctgct tgcagacaat
     1021 ccgatgctga aaatgatcag ctatgaaaac cagtcgcttg aggcacggaa aaagatgatc
     1081 tcaggcatga gttatccaat gatgggtatc ggaatcaatt attcgctgat caataaaagc
     1141 ccgatgtctg agtcagcgat gaacggttct gacatggtca tgccgatggt taccgttaca
     1201 atccctgttt accggaagaa gtataatgcc atgaaatccg agaccgatct gctcaaatct
     1261 tcatctgaga agagctacag ggcaactgaa aattccctga aggttgagct cttaagtgcc
     1321 atgctggact atcaggatat ctcgagaagg gttaaactgt acgaaagcca gcacagtctt
     1381 gctgccaggt ctcttgacct gatgctgaag gatttctctg cctccgcatc atcacttacg
     1441 gatgtattaa gattgcggca acagatttat gaatatgaac ttaacctgat tgaagccagg
     1501 gcggatctga atattacttc tgcccggata acacggctca tggcatacac aatataataa
     1561 ttagaatcac acaaatggaa aaacttaata attctcagtt aaaccgtctg ttctccaaca
     1621 ggtatttcaa ctacaccctc tttgtccttg ccggtctctt tcttggctgg ctgattttcc
     1681 gtgacgggca cggtcatgat catgaaccgc gacaatccac agaagtggca caggaaacaa
     1741 tatggacatg ctcaatgcac ccgcagataa gaatgcagga acaaggcgac tgccccatct
     1801 gcggcatgga cctgatacct ctcgctcaga acggttcctc cggcattgat cctgatgcag
     1861 tacatctcac aaaggagggt gcagccctcg caaatgttat gaccactgtg gcatcaaggc
     1921 agaagcctgt catggaggtg aggctttacg gcaaggtgca ggctgatgaa agattgttgc
     1981 agaaccaggt gtcacatgta tccggaagga tagagaagct gttcgtgaat tttacaggtg
     2041 agagtgtcag taaaggccag ctccttgcac aggtgtactc cccggaattg gtttcagccc
     2101 agcaggaact gattgaggcg gcgaagacga aagagactca gcctgccatt tatgatgccg
     2161 ccagggaaaa acttcgcctc tggaaaatta ctgatgcaca gatagagaca attgaaaact
     2221 caggaatcgc tcagtcactg gtcgatattg tatcaaacac tgatggcaca gtaatctcca
     2281 ggctggtgaa tactggtaat tatatctcac agggaacgct gttatatgaa attgctgacc
     2341 tctccgggct atgggtgctc tttgatgcat atgagagtga tctccggttc ctgaaaagag
     2401 gcgaaagact cacattcaca gtcgaggcat atccgggtga gaaattcacc gggacaattg
     2461 cctttattga tccggttatt gacccggtga cacgcgtggc gagggttagg gttgaaaccg
     2521 ggaacagatc aggcaggcta aaaccggaaa tgtttgtaac aggtatcttc tcaaccgctt
     2581 tgcctgagtt cagcaataat ctgattattc caaaatctgc ggttctctgg accggcaaac
     2641 ggtcaattgt atatgtcagg cagcccggga ttgatgaacc ggtctttaca atgcgtgaag
     2701 tagaactggg gccggcgctt ggagacagtt atgtcgttgt ctcaggtctg gcagagggag
     2761 aagagatcgt caccaacggc accttcagtg ttgatgctgc cgcacagctt gcgggcaagc
     2821 caagcatgat gaatcccgtc gggggagcct cctccacagg tcatgatcac ggtacaatgt
     2881 cagaaactgg aagcgtcgaa gggaatacca cagaatcaga ccacgatcat agcacgacag
     2941 ccaccactgg tgacaatctg tctgccagca cgggccatac cgaattcagg gttgaaggaa
     3001 actgcgagat gtgcaaggag aggatcgaaa ccacggcaaa aaaagtgcat ggagttgaga
     3061 ctgcaaactg gattattgat acaaaacaac tgcatctcac ctttgacaaa tcacatacag
     3121 atcttgacga agtgaaaaaa gcaatcgctg atgccggaca tgataatgac aaataccggg
     3181 cacccgacac tatctataac agtttaccgg agtgctgtct atacaggaaa taatgagtaa
     3241 tcccgtaaaa ttatggaaca aaaaataaca aacctgattg acctcctcag acaacacaaa
     3301 agtgctgtga ttgcattttc aggaggggta gacagcactt tcctggccag gatcgccagg
     3361 gatgcttacg gagacaacct cctcctgata actgcaacgt caagtaccta cccttactat
     3421 gagctcgacg aggccagatc acttgcggaa ctgctcggaa taaagcaccg gataatagtt
     3481 tccgaagaaa ttgaaatccc cggctatgct gacaatccgc ctgacagatg ctattactgt
     3541 aagagtgagc ttttcgggaa aataaaattc attgctgagc aggagggcta tgaggcagta
     3601 tttgacggca gcaacgccga tgaccttaaa gactatcgtc ccggaatgag ggctaaacag
     3661 gaaaagggaa tcatctcccc tcttgcagag acagggttta caaagaatga catccgccat
     3721 ttctccgccc ttatgaatct gccgacggcc acgaaacagt catatgcctg cctggcgtca
     3781 cggttcccct acggcgaaaa gataacgata gaaaaacttg accgcgtggg tgcggcagag
     3841 tttgaaatca ggaagatgga attcacccag ttcaggattc gaagccacga aaaccttgcc
     3901 cgccttgaat tcatcccgtc cgaaatggat aaggcctgga ataacaggga ccatctctca
     3961 ctgatatgca aaagatccgg gttcagctat accacgattg atttaaccgg ctacaggacc
     4021 ggctcaatga atgaagtcct tcctgaatca gtcaggaatg aaaggaatga gaatcactga
     4081 actactttat taaaagacat atctgaatga cctcatccag aaacttaacc cgggaagaaa
     4141 aggcaagata cagcaggcag atcaggctgg ctgaaatcgg tgaggagggg caactgaagc
     4201 tgaaggcatc atccgtgctg gtcatcggtg ccggggccct gggatgcatc gtgctgcagt
     4261 accttactgc agctggaatc ggtacaatcg gcatcgttga caatgactgg attgatgtct
     4321 ccaacctcca ccgacaggtt ctttatgata tcagcgacat ctcaaaacct aaaatactgg
     4381 cttcaagggc aaggcttgaa cgcctcaacc cggaggtgaa attcaacaca tacttcctga
     4441 ggcttaacaa ggacaatgcg gctgatatta taaaggagta cggtataatt attgactgta
     4501 cagacaactt tgccaccagg taccttatta atgatgtttg cgttttgctg aacaaacctc
     4561 tggtttatgg tgcgatcctt caattctcag gccaggtaat ggtactgaac taccgtaacg
     4621 gcccgactct cagatgcctg tttcctgatc ctcctcaccc gcttgaagtt ccttcctgtg
     4681 cagaggcagg tgttatcggt tcaattgcag gtataattgg ttcaatccag gctacggagg
     4741 tgattaaaat gatcgtcggt gccggagaga tcctctccgg gaaaatgctg atactcgatt
     4801 cccttaacta ttcaaccgag atcatagctt ttgaaagaga cccggaaagc tcagtggttc
     4861 acagccttgg tgaatacgaa gatgcctgcc tttcggaaga tgactccgtg aatgaaatat
     4921 ccgctgagga tctgagaaag atgctttcgg tcaaccctgg tacgaaagtc attgacctga
     4981 gggataaatc agatcctgaa aacccggagt tcgaaagcat aaggatacct tattatgaaa
     5041 taagccgaag acctgacatt tttaccgggt cagaaccggt tgttttctgg tgcaggtatg
     5101 gaatcaaaag caggcaggtg attaattatc ttaaaaagta tcatggacga aacaacttat
     5161 acagccttgt catctgaccg aaaccagatt atcagtctgc tggcctcgac cggcgactta
     5221 cggaaatctc tctttgagca cgcggcaagg gtaaagaggg aacatgtcgg tgacctggtc
     5281 tatttcaggg gattgatcga gttttcaaac aactgccgga agaactgcct ctactgcgga
     5341 ataaggtcgg gaaacaggaa ggtaaaacgg tttaatcttt ttgatgagga aatcgtcagt
     5401 gcggcaattt ttgccaatga caacggttat ggctcaattg tgctccagtc aggtgagatc
     5461 catgggaaga tgttcacgaa gagaatcgaa tccctcctga agaaaatcca tgctgcgaca
     5521 aatgacaggc tgaggataac actttcatgt ggcgagcagg agaaagatgt ctacagacga
     5581 tggtttgaga gcggcgcaaa gaggtacctt atgaggatcg agacgtcaaa ccaggatctc
     5641 tatgcacgac tccatccgaa tgaccagaga cattccttca ggaaaaggct agactgcctg
     5701 aatatcctga aagagacggg gtaccagacc gggacaggtg taatgatcgg tttaccattc
     5761 cagaccccgg aggatcttgc agatgacctg ttattcctga aaaacaatga catagtaatg
     5821 atcgggatgg ggccctatct tgagcacgct gacaccccgc tctacaggta ccggtcacag
     5881 ctccttccgg ttaatgaaag atttgacctg acactgaaaa tgatcgcaat cctccgtatc
     5941 atgatgaagg atgtgaatat cgctgctacc accgccctcc agacaataga caaacttgga
     6001 agggagaaag cgatcatggc cggtgccaat gtgataatgc ccaatattac accgggcatg
     6061 tatcgaaatg actactcact ttatgataat aagccctgca ccgacgaaaa tcccgaggat
     6121 tgcaggacat gcctggaggc acgtatagct ctgacaggca acaatatcgc ttttggcgaa
     6181 tggggagatt ccgctcactt tcccggttta taggagcaaa cagtattctt atttgaatga
     6241 gctatttgaa attactctaa atgatatttc ggttttttca ttataattgc attgattcca
     6301 ggtgaataat gataaaaagg ataacagtca ttctgatttt tatgctggca ggcatcctcc
     6361 tggtggctca tgctgttgta ccgcatcatc atcacgataa acaggtctgt ttcgaaaaat
     6421 ctcattgtat acacgaagat agtgatgagg aacctggcac cagtcatgac ggccacagtc
     6481 atgacggtga aaataaccat aatgactgcg ctttaaatga tccggttgta ataaccacaa
     6541 atgagtggaa acctgatttt aaattaattc aggcatctga tcgtacaggt tttgacgggc
     6601 ttcatgtaag cctgctgaac aatagttcag gatgtctgac tccctcatta tcatcctgtg
     6661 tctatgagcg ggtcacagac agtttatttc cttctgcagt ccctgattct catggattgc
     6721 gggcaccacc tgcagcttaa ggcttcccgt tgatttattg tgtacagtac gtcagcaact
     6781 gatgttttgc cgcatcacct ttttccgtct tataactctt aaacaaactg ttatgtattt
     6841 caagttatta ctgacctcat tgattgtgat tacagtcgcc ggatgcaacc atagccatga
     6901 aaaaggatct gatactgaat ccgaagaaat taagtttcag tatactgcat acagcgatca
     6961 gtatgaactg tttgcagagg ccgacccatt cattgccggt cagactgcta atgttctgtc
     7021 acatctctcc aaccttcctg aattcacagc tgtaacaagc ggtgaggtaa caattaaact
     7081 cattgttaac gggaaatcag tggaacagac actggagagc ccttcacgta aagggatcta
     7141 cagctttgat gtcaggccgg acgtgaccgg tactggcacc ctcgagtata ttgttaaaac
     7201 ggaaaccgat gattttaaaa ttagtgtacc ggaggtcgtt gtttatgccg gtaatcagga
     7261 ggcagcaaca gctgccgcaa tgaacaaaac cgcaagaacc aacaccacgg tttttacaaa
     7321 agaacaatca tggaaaacag aattctcaac cggttaccca gagagggaac cgttcggtca
     7381 ggttataaag accacggcac aaattcaatc cgcccagggc gatgaagcac ttgtgacagc
     7441 gaagacaagc ggcgtcatat cattacaggg aaatagcctg acggaaggaa tgaatgtgtc
     7501 aaatggccag gcacttttta tgattaaagg cagcggactc gctgaaaata attcaactgt
     7561 ccgttttgcc gaggcacaga ataattttga aaaggccagc gcagattatg aaagagcaag
     7621 actgctggct gaagataaga tcgtatctga aaaacaatta cttgaaacaa agacaattta
     7681 tgagaatgct aggtcggttt atgaaaatct gaagcagaat ttcagtgctg ccgggcaaac
     7741 agtcttcagt ccgatgagtg gtttcgtcaa gcaacttctt gtgaggaacg ggcaatatgt
     7801 cggggcagga gagccattga cagtgatatc acnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nncttgtgag gaacgggcaa tatgtcgggg caggagagcc attgacagtg
     7981 atatcacaaa acaagtcatt gttgctgcgt gccgaggttc aacaaaagta tgcccgtgac
     8041 cttatgtcaa ttacagctgc aaacatcaga accattgaag ataacaagac ctataccctt
     8101 gaagaactta acgggaaagt tttatctgtt gggaggacaa ccacaacaga taactttctg
     8161 attccgttta ccctccagat aaacaatgca ggcagccttg ttccgggtgg ttttgtggag
     8221 ttgtacctga aaacagtttc aaactcaaag gctcttacta tcccggtttc ggcagttctt
     8281 gaagagcagg ggaacttttt cgtatatgtt caggttactc ccgaactgtt tgaaaaacgt
     8341 gaaatcaaaa caggagctac agatggaatc cgcactgaag tcatcagtgg cctggttgat
     8401 gatgagcgca ttgtattgaa aggagcggtc ctgatcaaac ttgcccaggc tacaggctcc
     8461 ctggacgcac attcaggtca tgtacattaa caaatcgcag gatcatgatt aacaatatca
     8521 taaagttctc actgaacaat aagtatctga ttatcttact ttcagtggta ctggtggttt
     8581 tcggaatgag aactgcttta aacatggatg tggatgtatt tcccgacctg accgccccga
     8641 cagttgtcgt gatgaccgat gcacacggca tggcatcaga ggaagttgag cggatggtga
     8701 catttcctat tgagacctcg gtgaacggcg ctaccggagt gcgaagagtc cggtcggctt
     8761 catcacaggg cttctccttt gtctgggtag agtttgactg gggtacggat attttcaggg
     8821 cacgtcagat agtgagtgaa aaactcatta caatttcctc ccggatgcca ttgggagtcg
     8881 gtcagccggt actggcaccg cagtcaagcg ttatggggga aatattcttt atcggagtcc
     8941 aggctgacag tacaagcctg atggatctga gaacaattgc agaatggaac atcaaaccat
     9001 tactgctggc aacaggagga gtctcccagg taactaatat cggcggagac tataagcaat
     9061 accaggtgct tgccaaccct cagaaaatga aattctacag ggtttcaatg acggaactgg
     9121 cagaggtgtg caaaggtctc agccaaaact caacgggagg tgtcgtaagg cagtatggca
     9181 atgaatatgt tgtaagaggt attgcccgga catctgattt aaccacttta ggcagttcct
     9241 atgtcaaatc cgtcaacggg aaaccggtga gattgaatga tgttgcagaa ataaaaattg
     9301 ggagcgcggt taaaatggga tatgcctccg aaaatgcaaa tccggcaata ataatctccg
     9361 tatccaagca acctaacacg aatacactta atgttacaga gagaattgaa gagaacctta
     9421 gaacattgca gaagacactt ccggctgatg ttaagctgga cacaaaaata ttccggcagg
     9481 cagatttcat tgaaacatct gtcagcaatg ttcagaaggc attgcttgaa ggtgcggtat
     9541 tcgttgtatt gattctgttc ctctttctgg gaagtttcag aaccacaatc atatcactta
     9601 tagcaattcc gctttcactg ctcggtgcaa ttctggtact gagacttctt gggttaaata
     9661 tcaatacaat gagccttggt ggcatggcaa ttgcaatcgg tgcacttgtc gatgatgcaa
     9721 ttattgacgt tgagaatgta tataaacgcc ttcgtcaaaa ccgtcagaaa cctgttgaac
     9781 tgcgacagga tgcttttaca gttgtatttg aggcatcaaa ggaaataaga gcctcgatcc
     9841 tgaatgccac attgataatt attgtagcat tcattcccct gttttttctc tcaggaatgg
     9901 aaggaagaat gctgaaaccc cttggaatct ctttcatagt ttccctcttt gtatcaatgg
     9961 ttgtggcaat gacacttaca ccattgatgt caaaaatgct tttgtcaaat gaccggtacc
    10021 ttgcaaggaa tgagaaggaa aaatggctcg tacgaaagct gtcatactac tatgaagaat
    10081 ctctcagatg gtcactgaac cataaaagat ctatcgtgct ttccaccctt ggattatttt
    10141 ttgctgcaat catcgcgatg tccactatgg gaagaagctt ccttcctgaa ttcaatgagg
    10201 gttcattaac cctgtctgta ataacgaagc ccgggacttc cctcgaggaa agcaacaacc
    10261 tgggaaatct tgtcgaaact gaattattgt ctatcccgga ggtatcaagt accgcaagaa
    10321 gaaccggacg cggagaactc gatgaacatt cgcaaacgac aaacagcgct gaaatcgatg
    10381 taaattttga ccttaatgaa cgcagtcgtg aagaatttat ggcagatgtc cggcggacct
    10441 tatccggaat ccccggaata gcgtttacag taggtcagcc ccttggacac cgaatcgacc
    10501 atatgctctc aggaacaagg gcaaatattg cagttaagct gttcggatca gacctcaata
    10561 aaatgttctc aattggtaat gagataaaaa gctctattgc tgatgttgaa ggtcttgtgg
    10621 atgtaaatgt cgaccagcag atagaaattc cccagataca gatcagggca aaccgggaca
    10681 tgcttgccca gtacggaata accacaaatg agtttaacga atttgttgat attgcattcg
    10741 gtggagagaa actggctgat atctatgagg gtcagcgaag ttttggcctc gttttacgga
    10801 tggatacgga ctatctggaa aacatggagg gcattcgcag tgcgctcatc gacacccatg
    10861 acggcaggaa agttccgctg gaacaggttg ctgatatagt atcggtgaca ggaccaagct
    10921 caatcagccg tgagaatgta cagcgaaaaa tagtagtatc tgcaaacgtg gcagggcgtg
    10981 atttgcgcgg agcagtgcag gatattcaga aaaatataaa tgagtcagta gtccttccgg
    11041 aaggctacag aatcgaatac ggcggtcagt ttgaaagtga ggcaaaagcc tcacaaaccc
    11101 ttatgctgac ctcattgctg gccctggtga ttatctttct gctgctgttt caggagttca
    11161 agaatttcaa actggcggga attatccttc tgaatcttcc attggctttg atcggcggcg
    11221 tatttgcaat ttactttacg tcggggatcc tgagtatccc cgcaatcatc ggattcatta
    11281 cgctcttcgg tatagctacc aggaacggga tcctgctggt ttcacattac cagcacctgc
    11341 aggcaagcgg aacttcattg acggacacag taaccaaagg ctctgcagac aggcttaacc
    11401 cgatattaat gactgccctt actgcagccc ttgcactgat tcctctggcc ctgcagggca
    11461 acctggcagg aaatgaaatt cagagcccga tggcaaaagt tattctcgga ggtctgataa
    11521 cctcaacact gcttaatatt tacatagtcc cgatagttta cagcgcactc aggacccggg
    11581 gaatcttaaa aaatgacaac ttatgaaacg attgattata gttctaagta cagttattct
    11641 gggcagtgca aatctgttgt cccaggatat tgacaggatt cttgccgggg tggaaaagaa
    11701 caacaccaca ctcgtcgctc tcaggaaaag tgcagatgct gaactcatct ccaataagac
    11761 cggtctgtat ccggagaacc cggaatttgc cttcaactat ctctggggca gcccggaaag
    11821 tatcggtaac cgaaaggata taagcatgat gcagtctttt gattttcctt cagcctatgc
    11881 ttacaggaat cagatatcgg aacaaaaaaa tgtccaggtg gaacttgaat acgagagaca
    11941 gcttaaatca atcatgactc aaacccgcct cgtgtgcaat gaattgatat accataatgc
    12001 cctggcaatt gagttaaagc aaagaattgt caatgcacag aaaattgcgg attcgtacaa
    12061 atcaaaattt gaggccggcg agacaggaat aattgatttc aacaaagccc aggtgtacct
    12121 gctgaatatc atcaaagatg cagaatcctg tgatattgaa aggagttccc atctggcaga
    12181 actgaaaagc cttaatggtg gcttaccggt tgacttcaat gacagtatct tcccgttaca
    12241 gactctgcct gttgactttg aacagtggta tgctgatgct gaaaaaaaca atcctgtgtt
    12301 gcgatggctg aatcaggaaa tctcaatcag tcagaagaat gaaaaattaa atgcagcaat
    12361 ggggcttccc aaaatacagg ccgggtacat gagtgaggat gttgtcgggg agcagttccg
    12421 gggagttgcg ttaggtctga cgcttccttt atttgagaat aaaaatgccg tgaaatatgc
    12481 aaaagcaaag acttttgcgg tgcaaagtgc tgagaccgac agcagacagc aattctataa
    12541 taacctgaag gcactgcata caaaggcagg cagcctgcag aacagcctga atgattatcg
    12601 gagcaatctt caaaaattca ataattctgc cctgctgcaa aaagccctgg acaacggaga
    12661 actttcgctc gctgagtatt accttgaact aactgtatac tatgaaagtt tcaacagact
    12721 gctggacata gaaaagagtc ttaatgagac tgttatcgaa ctattcagat accagtaagc
    12781 ttttatctca tgaaagaaaa tgaacagata ttctataaaa gggcattctg gttaagcctg
    12841 tttacaattt tttacaacat tattgaagga gtggtttcaa tggctttggg ttacgcggat
    12901 gagaccctga gcctgtttgg tttcggtgct gacagtttta tcgaagtgct gtccggcatc
    12961 ggaatagcaa ttatgataac ccggattagg cagaatcctg acagcccgaa gagcaggttc
    13021 gaaataacag ccctgaagat tacaggtacc gcattctatc tgttgtcagc aggactgttt
    13081 gcaggtatta tactgaatat catttataaa cacaaaccgg aaacaacaat ctggggaata
    13141 gtgatttcac tgatatcaat tgccgtgatg atctggctta tgaatgcaaa aaaaagcatc
    13201 ggcaaaaagc ttcattccga acctataatt tccgatgcca attgcaccag ggtatgtgta
    13261 tacatgtcag tagtgctgct tgtatcaagt cttgtctatg aatttaccgg atttgcctat
    13321 gctgatgcaa tcggggctgc aggccttatt tacttttcag tatcagaagg cagggaagca
    13381 ttcgaaaagg cgaaagggaa gagttgcagt tgcggcgaac actgtgaagc agattaggag
    13441 acctggcgtc aaaactacca aatggcggat atcaggtacc gggcttagaa gtgttcaaaa
    13501 acccccaggc cgaacagagc aaagtcatac ctcaccggat ccgcaggatc catttcccg
//
LOCUS       DAOPXU010000073        13505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_5015_length_13505_cov_12.690558, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000073 DAOPXU010000000
VERSION     DAOPXU010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13505
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5015_length_13505_cov_12.690558"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..1790
                     /locus_tag="PKK03_08340"
     CDS             252..1790
                     /locus_tag="PKK03_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNX84449.1"
                     /translation="MKRSYIVLLAIFFFLAISALVIIQVRWINNVVSAEDQQFRFGVN
                     EALKEVVSELEKAETYKRIMSGINPEPIPQDTDEPSEQSEQKSAEEKLLEKYGFDPGV
                     RSIIISRAGYTYLLDSDSLGVESFFESAEPDEQILSAGATSRMTSKVISIENIVSRIL
                     HETPPLRDRFTADELNSLIRRSLDAVGIHLDYECSVRGAYGDIIYRTPDYLESSGANK
                     YLRQLFPNDPVPGNNILSIYFPEEEEYKFSQIAFMASSSMLIVLLLTLLATGTFIVIF
                     RQKRLSEIRSDFINNMTHELKTPIATISLASQMLADPSIPESSRNTGSLATVIREESN
                     RLKVLVERVLHTAIFEKARLELNKQPVDLHALIIRSVDAFKLQVNGRGGTISTFLDAS
                     DPIVVLDEANFFNVLLNLIDNALKYTLSTPEITVTTAAWHRGIVITVSDNGIGIPREH
                     LKHIFEKFYRVPAGNTHNIKGFGLGLSYVKKIVEEHYGTIKVESQTAKGTRIIIHLPK
                     EQPK"
     gene            1787..2473
                     /locus_tag="PKK03_08345"
     CDS             1787..2473
                     /locus_tag="PKK03_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX84450.1"
                     /translation="MKKISILLAEDDPNLGQLLKNYLIAREYDTTLVTDGAQALQVFR
                     KDKYQLCLLDVMMPELDGFTLAKEIRALDPHIPVIFLTAKNLKEDVIEGFKSGADDYL
                     TKPFSMEELLYRIEAILRRASGKQADPPATSYTIGKFTFDVTKQLLTYNDQSRKLTTK
                     ESELLELLCRNRNEVLERNFALKSIWIDDNYFNARSMDVYITKLRKYLSRDESVEILN
                     IHGKGYKLLC"
     gene            complement(2662..4449)
                     /gene="lepA"
                     /locus_tag="PKK03_08350"
     CDS             complement(2662..4449)
                     /gene="lepA"
                     /locus_tag="PKK03_08350"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNX84451.1"
                     /translation="MDNIRNFCIIAHIDHGKSTLADRLLEMTRTVDARDFQDQVLDNM
                     DLERERGITIKSHAIQMSYQYEGKNYFLNLIDTPGHVDFSYEVSRSIAACEGALLVVD
                     ATQGIQAQTISNLYMAIEHGLEIIPVMNKMDSPSAMPEEVKDQIVDLIGCKREEIIEA
                     SARTGMGIDRIMSEIITKIPAPKGDPDAPLQALIFDSVFNSFRGIIAYFKIANGTIRT
                     HELVKFVNTGSEYDADEIGVLKLKREPRDVLRAGDVGYIISGIKVSAEVKVGDTITHV
                     ARPCDRAISGFADVKPMVFAGIYPVDADDYEDLRASIEKLQLNDASLTFVPESSAALG
                     FGFRCGFLGLLHMEIIQERLDREFDMDVITTVPNVSFRVLTTRGEIIDVHNPSGMPPS
                     TTIDEIEEPYIHAQVISKSEYLGSILKLCIDKRGTMKNQVYLTSDRVEVTFDMPLSEI
                     VFDFYDKLKSISKGYASFDYHITGYKKADLVRLDILLNGEMVDALSTLIYRGHAYDFG
                     RRMCEKLRELIPRQQFDIAIQAAIGAKIIARETVKAVRKDVTAKCYGGDITRKRKLLE
                     KQKKGKKRMRQIGNVEVPQQAFLAVLKLD"
     gene            4574..6055
                     /locus_tag="PKK03_08355"
     CDS             4574..6055
                     /locus_tag="PKK03_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNX84452.1"
                     /translation="MITLAVSVTLLILGYLVYGRVAERFFGMDPARTTPAFTKNDGID
                     YIPMKGWRVFMIQFLNIAGLGPIFGAILGAMYGPVVYIWIVLGCIFMGATHDYFGGML
                     SVRHGGASLPELVGTYLGKSMRMFLRAFTLFLLIFVGVAFVSGPAKLLFTMTGFSLPF
                     WLGLIFFYYILATLLPIDKIIGLLYPFFGAALIFMAAGVLGGLIVNLAGGNITLMELS
                     PASLVNFQPDAATNNIFPFLFIVVSCGAISGFHATQSPLMARCIRSEKQGRHIFYGAM
                     IAEGIVAMIWATAAMNYMGDVHGLNYAFTHPLADNPAIKPDPAWLVNEICRSWLGKVG
                     AVVAVIGVVACPVTSGDTAFRSARLTIADMLKIPQKKVSSRLLIVVPVFALAFILTFV
                     MKDEFARIWKFVGISNQALAAITCWTISMYLGLRGKSHLMMSLPALFLTAVCVTYLLA
                     APHTGGGLALPMKVSLPAGIAAAVAVFALFLAVLRKKRLQENQ"
     gene            complement(6049..7452)
                     /locus_tag="PKK03_08360"
     CDS             complement(6049..7452)
                     /locus_tag="PKK03_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNX84453.1"
                     /translation="MTDPRQVYELEHEKPGKLMWKYFLPAFASMMANALYNVVDRIYI
                     GQGVDALALSGLTVVFPLMIIIMAFGMLVGMGSAVRISLSLGEKDFDRANRILGNAFF
                     LSLVLGLVLMGTGFLVRDRVLQIFGAGPETIQYASDYFTIILLGTPMAMMGFSLNNVI
                     RAEGNPRIAMYSIFISAGLNIVLDPIFIFGLDMGVKGAAIATVISHFVLAVWVLVHFR
                     SKRSVTRLTAATFRPDFYIIKYIVSIGFAPFAMNLASSIVWGVMNSKFIKYGGDISVA
                     ALGIVNSATMMLVISIISINMAVQPIVGFNYGAGLFCRVKETMMKAIRYATLIAAGGW
                     LICMLAPGVIVSIFNTDSPELREAGTLGLRIYCAVLPVVGFQIIASNYFQAIGKARLA
                     TFLSLLRQIIVLLPLIFILPQFWGATGVWIANPISDFVAAVISFIFFRREIAKLNCDM
                     PVAGRRNPEIAPPSETY"
     assembly_gap    7542..7641
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7770..9404)
                     /gene="groL"
                     /locus_tag="PKK03_08365"
     CDS             complement(7770..9404)
                     /gene="groL"
                     /locus_tag="PKK03_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654643.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNX84454.1"
                     /translation="MSKEIKFNIEARNLLKEGVDRLADAVKVTLGPKGRNVVLEKKFG
                     APQITKDGVTVAKEIELSDAYQNMGAQMVKEVASKTSDTAGDGTTTATVLAQSIISVG
                     LKNVTAGANPMDLKRGIDKAVSTVVESLKSQSQSVGDDLKKIEQVARISANNDEEIGK
                     LIATAMDKVKKEGVITVEEAKGTETKVEVVEGMQFDRGYISAYFVTNPDKMEVEMDNP
                     YILIHDKKISSMKDLLPILEETARSGRGLMIIAEDVEGEALATLVVNRLRGSLKVCAV
                     KAPGFGDRRKEMLEDIAILTGGTVISEERGMKLENATMADLGIADKVTVNKENTTIVN
                     GAGEKDMIQARINQIKQQISTTTSDYDREKLQERLAKLAGGVAVLYIGAASEVEMKEK
                     KDRVDDALHATRAAIEEGIIPGGGVAYLRAIPLLDKLKGDNDDQNTGIEIVKRAIEEP
                     LRQIVTNAGLEGAVIVQKVREGKGDFGYNAQTDKFENLLKAGVIDPAKVARIALENAA
                     SIAGMFLTTEAVIVEEKEENPMPNPAMGGGGMPGMM"
     gene            complement(9426..9704)
                     /locus_tag="PKK03_08370"
     CDS             complement(9426..9704)
                     /locus_tag="PKK03_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNX84455.1"
                     /translation="MAQLKGKVLAGKILVKPHEAEEKTASGIIIPDSAKEKPRAGTVV
                     LVGAAKKDEEMELKKGDVVLYGKYSGQELNIDGADYLLLSQSDVLYIS"
     gene            complement(9837..10187)
                     /gene="secG"
                     /locus_tag="PKK03_08375"
     CDS             complement(9837..10187)
                     /gene="secG"
                     /locus_tag="PKK03_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNX84456.1"
                     /translation="MGIYIFLTILVILACVLVIMVVLVQNSKGGGLASNFTSAGQSMG
                     VRKTADFLEKSTWTLGAAILVLCLAATASIPRGQGAKESVIKSSIERAQDPNAIPVLP
                     TQTPVQEQPEGGNE"
     gene            complement(10195..11649)
                     /locus_tag="PKK03_08380"
     CDS             complement(10195..11649)
                     /locus_tag="PKK03_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84457.1"
                     /translation="MNRNEFNRFIAGIDLPGPGDLDELRELTSLFPWFHSAHMLLLRG
                     LKENNDIRFDSQLRVSALSVADREVLYHYLFLSPADVAGAEVVTTPAFTEVPCEEVPP
                     VVITATGETPVAEEVPDTEEIPVISADQPAEGTPVAEEVPGTEEVPGSEEVIVTEEVP
                     VTGETPVIFADQSEEEIPGPEEISVAGEESAGNVIDGAEDRQAEELTEKLRTREELIA
                     EIEARLRELEEIHKTAADQVAQQVPEQQIPVAGQMPEEMPATEPRPEEPPVTEPVMEE
                     VLMPESMTVEIPVHDLPPERMPESELSTEETPVTESAPEELPAPEPEPEEIPQEFPEQ
                     FPEEFPEPVTEPLPEPVPGIEIEWQTGTPAESESDAEPRQEELLELIPDQPEEESEPA
                     TQLTPADLIDRFIRISPSIERLTPGDVQPVKDLSAESTDEHGKFITETLAKIYINQGY
                     YSRAINIYERLSLQYPEKSAYFASRIEKIKDLIK"
     gene            complement(11658..12173)
                     /locus_tag="PKK03_08385"
     CDS             complement(11658..12173)
                     /locus_tag="PKK03_08385"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HNX84458.1"
                     /translation="MHRIVKIMVPLVLLAMTAGCGVKIQYKFTGASIPVEANTISVAT
                     FQNRASLVVPGLTQTVTDALIDMCRAQTNLDMVSSGGDLSFEGEITDYKTQPLTVSGN
                     EQAAMNRFSISVRVRYVNAFNDDNSFDQTFTRYQDYSSALDLSAVEASLTEEIVKMLI
                     DDIFNRAFVNW"
     gene            complement(12166..13416)
                     /locus_tag="PKK03_08390"
     CDS             complement(12166..13416)
                     /locus_tag="PKK03_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNX84459.1"
                     /translation="MLMKELQSVKQRFGIIGNHEGLNRAIDIAVQVAPTDLSVLITGE
                     SGTGKEVFPQVIHSFSMRKHGPYIAVNCGAIPEGTIDSELFGHEKGAFTGATDSRKGY
                     FEVANGGSIFLDEVAELPLSTQVRLLRVLETGEFIRVGSSKVQKTDVRVITATNVDIV
                     QAVGSGRFREDLFYRLNTVPVRVPPLRERGEDIILLFRKFAVDFAERYRMPAIRLDHQ
                     AREMLMNYYWPGNVRQLKNITEQISVIEQERDVTAAILAHYLPDIQTGRFPVPARAHA
                     DDKSFSSEREILYKVLFDMKSDMHDLKNLVYDLLTTGDADNLTESGARAVRNLYNQGS
                     LAGDQYEPAPPVKVSYSPPKESRVIEDTEEVIEESLSLSDREKELIKKALDKNNGKRK
                     LAAAELGISERTLYRKIKEYRLDA"
BASE COUNT         3138 a         3575 c         3248 g         3444 t
ORIGIN      
        1 tcgcctcctc ttcagtgtta accctaaaac taacctaacc tatgaaaaaa actccattta
       61 tgttatcaca attatcgtgc cataactgat gcaaatatca gaatattttt ccggacaagt
      121 tcttaaggat atgttaatat attgattttt aacatttttt taatactttg ctttttttac
      181 attgcccctg aacctgttaa agaacgttaa gtctatgtat taaagcttta acagattatt
      241 tttgttaaaa tatgaaacgc tcatatatag tcctcctggc catattcttt ttcctggcca
      301 tatctgccct ggtaataatc caggtcaggt ggatcaacaa tgtagtgagc gcagaggacc
      361 agcaattcag gtttggagtg aacgaagccc tgaaagaggt ggtatcagag ctggagaagg
      421 ctgaaaccta taagcgcata atgagcggca tcaatcccga accgattcca caggacaccg
      481 atgaaccttc agaacagtct gagcagaaat cagctgaaga aaagcttctc gaaaaatacg
      541 gcttcgaccc cggtgtcaga tcaataatta tcagcagggc cggctatacc tacctgctcg
      601 attccgacag cctgggagtt gaatcattct ttgaaagcgc cgagcctgac gagcagatcc
      661 tctcggccgg agctaccagc aggatgacca gcaaggttat ctccattgaa aatattgtga
      721 gcaggatact gcatgagact cctcccctgc gtgaccgttt cacggcagat gaactgaaca
      781 gcctgataag gagatcactt gacgccgtgg gcatccatct tgattatgag tgttcggtac
      841 gaggcgccta cggcgacata atctacagaa cccctgacta cctggaaagc agcggggcca
      901 acaagtacct ccgccagctg ttcccaaatg acccggtgcc cggaaataat atactttcga
      961 tatattttcc cgaggaggag gaatataaat tcagccagat cgcgttcatg gccagctcat
     1021 cgatgctgat agtcctgctg ctgacccttc tggctacagg aaccttcatc gtcatcttcc
     1081 gccagaagag gctgtcagag atacggagcg acttcatcaa caacatgact catgagctta
     1141 aaacgccgat agctaccata tcactggcat ctcagatgct tgccgatccc tccatccctg
     1201 agagctcgag aaataccggc agccttgcca cggtcatcag ggaagagagc aacaggctga
     1261 aggtgctggt tgagagggtg ctgcatacgg caatctttga gaaagccagg cttgagctca
     1321 acaagcagcc ggttgacctg catgccctca ttatacggtc agttgatgcc ttcaaactcc
     1381 aggtcaacgg acgcggcgga accatctcaa ctttccttga cgcctccgac ccgattgtgg
     1441 tacttgatga ggccaacttc ttcaatgtac tgctcaacct cattgacaat gccctcaagt
     1501 acaccctgag cacacctgaa ataaccgtaa ccactgcagc atggcacagg ggcatcgtca
     1561 tcaccgtatc agacaacgga atcggcatac cacgagagca cctgaaacat atatttgaaa
     1621 agttttaccg cgttcccgcg ggaaatacac acaatatcaa aggattcggc cttggactca
     1681 gctatgtgaa aaagatcgtt gaagagcatt acggcaccat taaagtggag agccagaccg
     1741 caaaaggaac cagaattatc attcaccttc caaaagaaca gccaaaatga agaaaatcag
     1801 cattttactg gcagaagacg atcccaacct cgggcagttg ctgaagaact acctcatagc
     1861 aagggagtac gatacaacac tggtcactga cggtgcacag gccttgcagg tcttcaggaa
     1921 agacaaatat caactttgcc tcctcgacgt gatgatgcct gaactagacg ggttcacgct
     1981 ggcgaaagag atcagggcgc ttgaccctca catcccggtc atcttcctta ccgcaaagaa
     2041 tctcaaggag gatgtgattg aaggattcaa atcgggcgct gatgactacc ttaccaaacc
     2101 gttttcaatg gaagagctgc tgtaccgtat tgaggccata ctgcggcgtg cctccggaaa
     2161 acaggcagat cctccggcaa catcatacac catcgggaaa ttcacctttg acgtgactaa
     2221 acagctgctg acatacaatg atcagtcgcg caagctcacc acaaaggagt ctgagctcct
     2281 ggagctgctc tgccgcaacc ggaatgaggt gcttgaacgc aatttcgccc tgaagtcaat
     2341 ctggattgat gacaactatt tcaatgcgcg aagcatggac gtttacatca ccaagctgcg
     2401 caagtacctg agcagggatg aatcggtgga gatactgaat atccacggca aaggatacaa
     2461 actgctctgc tgagcatgac cattcataaa gcgggcaggg ctgccgaaaa gtaaataccc
     2521 cgcccggata atccgaacgg ggtatgagtc taggttagtt tcagggtctt tcgacttgtg
     2581 taatattctt cccgtcaaag cccaacgaag cgctaagtta gtataattta tttatttgca
     2641 ataccccctt catttttttt atcagtcgag cttaagtact gccaggaaag cctgttgcgg
     2701 cacctccacg ttgccaatct ggcgcattct cttcttgccc ttcttctgtt tctcaagcag
     2761 ttttctcttc ctggtgatgt caccgccgta gcattttgcg gtcacatcct tgcggactgc
     2821 cttgactgtt tcgcgggcaa ttatcttggc tccgatggct gcctgtatgg caatgtcaaa
     2881 ctgctgtctc ggtatcagtt cgcgcagctt ttcgcacatc ctgcggccga aatcatatgc
     2941 atgacccctg tagataaggg ttgagagtgc atcaaccatt tccccgttga gaagtatgtc
     3001 aagcctcacc aggtcagctt tcttgtaacc ggtgatgtga taatcgaacg aagcataacc
     3061 cttcgagatg ctcttcagct tgtcatagaa gtcaaacacg atctctgaca ggggcatgtc
     3121 aaaggtcacc tccaccctgt cactggtaag gtaaacctgg ttcttcatcg tacctctctt
     3181 gtcaatgcag agcttgagga tggatccaag gtactccgac ttcgaaatca cctgggcatg
     3241 aatgtagggt tcttcaatct cgtcaatggt tgttgacggt ggcatgcctg aggggttgtg
     3301 cacgtcaatg atttcgcccc ttgttgtcag cacacggaat gagacgttcg gcacggtggt
     3361 tatgacatcc atgtcgaact cgcggtcgag ccgttcctgt attatctcca tatgcagcag
     3421 cccgaggaag ccgcacctga agccgaatcc cagtgcagcg gatgattcag ggacgaaagt
     3481 cagtgacgca tcattgagct gcagcttttc aattgatgcc cgaaggtcct cgtagtcatc
     3541 cgcgtcaacg gggtaaatac ctgcaaagac catcggtttc acgtcggcga agccggagat
     3601 ggccctgtca cacggtctgg ccacgtgggt tattgtgtca cccactttta cctcggcaga
     3661 gaccttgatg ccggaaatga tatagcccac gtcaccagct ctaagcacat cgcgcggctc
     3721 gcgcttaagt ttcagcacac ctatctcatc ggcatcatac tcgctgccgg tgttgacaaa
     3781 cttcaccagc tcatgggtcc tgatggtacc gttggctatt ttgaagtagg ctatgatccc
     3841 cctgaatgag ttgaaaaccg agtcaaagat gagcgcctga agaggtgcat ccggatcgcc
     3901 cttgggggca ggtatctttg tgatgatctc tgacatgatc cggtcaatgc ccatcccggt
     3961 gcgggcgctg gcctcgatta tctcttcgcg cttacagccg atgaggtcaa caatctgatc
     4021 cttcacctct tcgggcatgg cgctgggtga atccatcttg ttcatcacag ggatgatctc
     4081 cagcccgtgt tcaatggcca tgtaaaggtt ggatattgtc tgagcctgaa tcccctgggt
     4141 ggcgtcaacc accagaaggg ctccttcgca ggcagcgatg gaacgcgata cctcatatga
     4201 aaaatcgaca tgccccgggg tgtcaatcag attgagaaaa tagtttttcc cctcatactg
     4261 ataagacatc tggatggcgt ggctcttgat ggtgattcca cgctcacgct caagatccat
     4321 attgtcaagg acctggtcct ggaaatcccg cgcatcaacg gtcctggtca tctcaagaag
     4381 cctgtcggca agagtgctct tgccatggtc tatgtgcgct attatgcaaa agttgcgtat
     4441 gttgtccatc agccgccggg cggttcaatt caggtgcaaa tatatataat ttgacccttt
     4501 cttaattgac cccgggatta tgataccgta ctgcattttc tgttaattta gcagattact
     4561 taatcaggaa cggatgataa ccctggcagt gtcggtcacg ttgcttatcc tgggatatct
     4621 tgtctacgga agagtggccg aaagattctt tgggatggac ccggcgcgga ccactcccgc
     4681 cttcacgaaa aatgacggta ttgattacat ccccatgaag ggatggcggg tcttcatgat
     4741 ccagttcctc aacattgcag gcctgggacc gatctttgga gcgatactgg gagccatgta
     4801 cgggccggtt gtctatatct ggatagtgct gggatgcatc ttcatgggtg ccacacatga
     4861 ttacttcgga gggatgctct cagtgcgcca cggcggcgcc agcctccctg aactggtcgg
     4921 cacctacctc ggtaaatcca tgaggatgtt cctcagggcc ttcacccttt tcctgctgat
     4981 attcgtcgga gtggctttcg tgagcggacc ggcgaagctg ctttttacaa tgacaggctt
     5041 cagcctcccc ttctggctgg gattgatttt tttctattat atacttgcca cgctgctccc
     5101 tatagacaag ataatagggc tgctctatcc cttctttggc gcagctctga tattcatggc
     5161 tgccggagtt ttgggcgggc tgatagtcaa cctggccggt ggcaacatca ccctgatgga
     5221 gctttccccg gccagccttg tcaacttcca gcctgatgcc gccaccaata acatcttccc
     5281 gttcctgttt atagtggttt catgcggcgc aatatcagga ttccatgcta cacagtcgcc
     5341 cctgatggcc aggtgcatca ggtcagagaa gcagggaagg catatatttt acggcgcaat
     5401 gatcgccgaa ggcatcgttg caatgatatg ggcaacagcg gccatgaatt atatgggtga
     5461 cgtgcatgga ctgaattatg cctttaccca cccgctggct gataacccgg caatcaagcc
     5521 cgacccggca tggctcgtga atgagatatg caggtcatgg cttggcaagg ttggcgcggt
     5581 ggtagccgtg attggtgttg tggcatgccc tgtgacctcg ggcgatactg ccttcaggag
     5641 cgccagactg accattgccg atatgctgaa aataccacag aagaaggtat catcaaggtt
     5701 actgatagtt gtgccagtgt ttgcgcttgc cttcattctg acctttgtca tgaaggatga
     5761 gtttgcccgg atctggaaat ttgtaggcat ctcaaaccag gccctggcag ccatcacatg
     5821 ctggaccatc tcaatgtatc taggcctcag gggcaagagt caccttatga tgtcactccc
     5881 ggcactgttt cttacagcag tctgcgtaac ctatctgctg gcggccccgc acacgggtgg
     5941 cggactggcc ctgccgatga aggtgtcact tccggcaggc attgcggccg cagtcgcggt
     6001 gttcgcgctt ttcctggcag tactgagaaa aaagaggttg caggagaatc agtaggtttc
     6061 tgacggaggt gcaatctccg ggtttcttcg cccggcaacg ggcatatcac agttaagctt
     6121 cgcgatctcg cggcggaaga aaatgaatga gataaccgct gccacgaaat ccgagatggg
     6181 attggctatc cagaccccgg ttgcgcccca gaactgcgga aggatgaaaa tcagcggaag
     6241 aaggacgatt atctgcctga gcaatgagag gaaggttgcc agcctggcct ttcctattgc
     6301 ctggaaataa ttggaagcta tgatctggaa tccgacaaca ggcagcacgg cgcagtagat
     6361 gcgaagccca agtgtgccgg cctcgcgcag ctccgggctg tctgtattga agatgcttac
     6421 tattaccccg ggagcaagca tacatatcag ccatccaccg gcagcaatca gggtggcgta
     6481 cctgatcgcc ttcatcatgg tctccttcac acggcaaaag agaccggcgc catagttgaa
     6541 tccgacaatc ggctgcaccg ccatatttat ggagatgatc gagatcacca gcatcattgt
     6601 ggcgctgttg acaattccaa gggctgcgac ggagatgtca cctccgtact taatgaactt
     6661 tgagttcata accccccaga caatgcttga ggccaggttc atcgcaaacg gggcgaatcc
     6721 aatggagact atatacttaa tgatatagaa gtctggcctg aatgtggcag cggtgagacg
     6781 ggtcacgctc cttttactgc ggaaatgtac cagtacccaa acggccagca cgaaatgcga
     6841 gatcaccgtg gcaatggcag cccctttaac acccatgtca agcccgaaga tgaagatagg
     6901 gtcaagaacg atgttcagcc cggcactgat gaatatggaa tacatggcta tccgcggatt
     6961 accctctgcc ctgattacgt tgttcaggga gaagcccatc attgccatgg gtgtgcccag
     7021 gaggatgatg gtgaagtaat ctgaggcgta ctgaatggtt tcaggacctg ctccgaatat
     7081 ttgcaatacc ctgtcacgca ccaggaatcc tgttcccatc agcaccaggc ccagaacgag
     7141 cgatagaaag aatgcatttc ccaggatgcg gttagcccgg tcgaagtcct tctcaccaag
     7201 gctgagtgag atgcgtactg ccgagcccat gccgacaagc attccgaagg ccatgatgat
     7261 tatcatcagc gggaacacca ccgtcaggcc tgacagtgcc agggcgtcaa ccccctgacc
     7321 gatatagatg cggtcaacca cgttgtagag cgcgtttgcc atcatgctgg caaatgcggg
     7381 gaggaagtat ttccacatca gtttccccgg tttctcatgc tcgagctcgt atacctgcct
     7441 ggggtcagtc ataaatgcaa agatggggaa aattggctgg atttgcgatt tgcgaattgc
     7501 gatttgcgaa ttgggatttc ggatttgcga tttcggattt gnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn ngatttcgga tttgcgattt cggatttgtg acttgcgaat
     7681 ttttgattcg ccaatctgaa atcgcaaata aaaaaactgc cccgggatcc ggggcagcct
     7741 ttccaattaa atcaataagc ttcttctttc tacatcatgc cgggcattcc gccgccgccc
     7801 attgcggggt tcggcatcgg attctcctct ttctcctcga caatgactgc ctcggtggtg
     7861 aggaacatac ctgctatcga tgcggcattt tccagagcta tcctcgcaac cttggccgga
     7921 tcaatcacac cagccttcag aaggttctcg aacttgtcag tctgagcatt gtacccaaaa
     7981 tcgcctttac cttcgcggac tttctgcaca ataactgcac cttcgaggcc ggcattggta
     8041 actatctgac gaagaggctc ctcaatagcc ctctttacaa tctcaatacc ggtattctgg
     8101 tcatcattgt cacctttcag tttgtcaagc agggggattg ccctgaggta agccactccg
     8161 ccgcctggga tgataccctc ttctattgca gcacgggtag cgtgaagagc atcgtcaacg
     8221 cggtccttct tctccttcat ctccacctct gatgcagcac cgatgtagag aactgccacg
     8281 ccgccggcca gtttggcaag cctctcctga agcttctccc tgtcgtagtc tgaggtggtg
     8341 gtggatatct gctgcttgat ctggttgatc ctggcctgga tcatatcttt ctcacctgca
     8401 ccgttcacaa tggtggtatt ttccttattg acggtcactt tgtcagcgat gccaaggtca
     8461 gccattgtgg cattttcgag cttcatgcct ctttcttccg agatgacggt accgcctgtc
     8521 agaatggcta tgtcttcaag catctccttc cttctgtcgc caaaacccgg ggctttgaca
     8581 gcacaaacct tcagtgagcc tctcaggcgg ttcaccacca gggtagcaag agcttcgccc
     8641 tcaacatcct cggcaatgat catcagacca cggccgctgc gtgcggtctc ctcaagtatc
     8701 gggagaaggt ctttcattga tgagatcttc ttgtcatgga tcagaatgta tggattgtcc
     8761 atctcgactt ccattttgtc aggattggtg acaaaatagg cagagatata tccgcggtca
     8821 aactgcattc cttccacaac ctcaactttg gtctcggtac cttttgcttc ttcaacggtg
     8881 atgacgcctt ccttcttcac cttgtccatt gcggttgcga tgagcttgcc tatctcttca
     8941 tcgttatttg ccgaaatgcg ggcaacctgt tcaatctttt tcaggtcgtc accaacgctc
     9001 tgtgactggc tcttaaggct ctcaacaact gttgacacag ccttgtcaat tcctctcttg
     9061 aggtccatcg ggttagcgcc ggcagttacg ttcttgagtc ctacgctgat aatgctctgc
     9121 gcaagaactg tagcggtggt agtaccgtca cctgcagtgt ctgaagtttt tgatgcaact
     9181 tccttgacca tctgtgcacc catattctgg taagcgtcag agagttcgat ctcctttgca
     9241 acggtcacac cgtccttggt aatctgggga gcaccgaatt tcttttccaa tactacgttg
     9301 cgtcccttgg gaccaagggt tactttaacg gcatccgcaa gacggtcaac accttccttc
     9361 agaaggttgc gagcttcgat attgaatttt atttcctttg acattgttat tcttttttaa
     9421 ggttgttacg aaatatagag aacatcagac tgagaaagaa ggagatagtc tgcgccatcg
     9481 atattcagtt cctggccgga gtatttcccg tagagaacaa catctccctt tttaagttcc
     9541 atctcttcat cttttttcgc agcaccaaca agaacaacag tgcctgcgcg cggtttttcc
     9601 tttgctgagt cggggataat aatgccactg gcggtctttt cctctgcctc atgaggcttg
     9661 acgaggatct tgcctgccag cacttttcct tttaattgtg ccattgctag tagttttata
     9721 gttaaacaat atttttcctg cttcagaatt tcataatctg tgccaaaggg aagttggcaa
     9781 aaaaaaagtg tcagttcatg acaaactgac actccgctgt cttcacggtg tcattattat
     9841 tcgttaccgc cttcaggctg ctcctgtaca ggtgtctgtg taggaagcac cggtatggcg
     9901 ttgggatcct gtgctctttc aattgatgac ttgatgaccg actctttcgc accctgtcct
     9961 ctcggaattg aggcagtggc tgccaggcaa agaaccagaa tggcagctcc gagggtccat
    10021 gttgatttct caagaaagtc tgctgtcttg cgtacgccca tcgactggcc ggctgaggtg
    10081 aagttcgatg cgagaccgcc tcctttggag ttctgaacca gcaccaccat aattacgagt
    10141 acacatgcaa ggataaccag gatcgtaagg aaaatataaa tacccattgt tatactattt
    10201 tattaaatct ttgatctttt caatacggct tgcaaagtaa gcactttttt ccggatattg
    10261 caatgagagt ctttcataaa tatttattgc cctggaatag tagccctggt tgatgtaaat
    10321 ctttgccagt gtttccgtta tgaatttgcc gtgttcatcc gtgctctctg ccgacaggtc
    10381 ctttaccggc tgaacatcgc ccggagtgag cctctcaatt gaaggactga tcctgataaa
    10441 acggtcaatc agatcggccg gggtcagctg cgtggcaggc tcagactctt cctccggctg
    10501 atcagggatc agctcgagga gctcctcctg ccggggttcg gcatcggatt ctgattctgc
    10561 cggagtacct gtctgccatt caatctcaat tccgggaaca ggttcaggga gtggttccgt
    10621 gaccggctca gggaattcct cagggaactg ctcagggaat tcctgtggaa tttcctcagg
    10681 ctcaggttcc ggtgccggca gttcctccgg ggcagattca gttaccggtg tttcctcagt
    10741 tgaaagttcg gattcgggca tcctctctgg tggaaggtca tgcaccggta tttccaccgt
    10801 catggattca ggcatgagga cctcctccat gacaggctct gttaccggcg gctcctccgg
    10861 ccggggctcg gttgccggca tttcctccgg catttgtccg gcaacgggga tttgctgctc
    10921 cggcacctgc tgagccacct ggtcagcagc tgtcttgtga atctcttcaa gttccctgag
    10981 ccgcgcttca atctcagcaa tgagctcctc cctggtgcgt agtttctccg tcagctcctc
    11041 tgcctgccgg tcttcagcgc cgtcaatgac attaccagca ctttcttcac cggctacaga
    11101 aatttcctcg ggaccaggga tttcttcttc agactgatca gcgaaaatga caggggtctc
    11161 cccggtgacc gggacctcct cagtgacaat gacttcctct gagccaggca cctcttcggt
    11221 gcccgggacc tcctcagcta ccggggtccc ttccgcaggc tgatccgcgg agataacagg
    11281 aatctcctca gtgtccggga cctcctcagc taccggagtc tccccggttg ccgtgataac
    11341 caccggggga acctcttcac acggcacttc agtgaacgcc ggtgtggtaa caacctctgc
    11401 ccctgcaaca tcagcaggag aaagaaacaa atagtggtaa agcacctccc tgtcagccac
    11461 tgaaagagct gatacccgga gctgcgaatc aaaccggatg tcattattct ccttcagccc
    11521 ccgcagaaga agcatatggg cggaatgaaa ccagggaaaa agggaagtaa gctccctcaa
    11581 ctcatccagg tctcccggac ccgggagatc aatgcctgca atgaacctgt tgaattcatt
    11641 cctgttcatg agcagaacta ccagttgaca aacgccctgt taaatatatc atcaataagc
    11701 atcttcacga tctcctctgt caggctggct tcaacagcac tcagatcaag cgcgctgctg
    11761 taatcctggt aacgggtgaa ggtctgatca aagctgttgt catcattgaa cgcgttcaca
    11821 tacctgaccc gcacggagat tgagaacctg ttcatggcag cctgctcatt gccgctgaca
    11881 gtgagcggct gagtcttgta gtcagtgatc tctccctcga aactcaggtc gccccccgat
    11941 gataccatgt caagattggt ctgcgccctg cacatatcga taagagcatc ggtaacggtc
    12001 tgtgtcagtc cgggtactac caggcttgcc ctgttctgaa aggttgcgac actaatggta
    12061 tttgcctcca ccggtatgga agcgccggtg aatttgtatt ggattttcac tccgcagccg
    12121 gcagtcatcg ccagcagaac cagcggcacc attatcttta ctatcctatg catcaagcct
    12181 gtactccttt attttcctgt agagagtcct ttcggagatc cccagctcag ccgccgccag
    12241 ctttctcttg ccgttgtttt tgtcaagcgc cttctttatg agttcctttt cacggtcact
    12301 cagtgagaga gactcctcta tcacctcctc ggtgtcctca atcaccctgc tctccttcgg
    12361 cgggctgtag ctgaccttca caggtggtgc cggctcatac tgatccccgg caagcgaacc
    12421 ctggttatac aggttgcgca cagccctcgc cccgctttct gtcaggttgt cagcatcacc
    12481 tgttgtcagc aggtcatata caagattctt cagatcatgc atgtcgctct tcatgtcgaa
    12541 cagcacctta taaagaatct ccctctcgct gctgaagctc ttgtcatcgg catgcgccct
    12601 cgcaggtacg ggaaaccgtc cggtctgaat atccggaaga taatgcgcaa gtatggcggc
    12661 agtcacatca cgctcctgct caatgacaga tatctgttca gtgatatttt tcagctgcct
    12721 tacgttgccc ggccagtagt agttcatcag catctccctc gcctggtgat cgagcctgat
    12781 ggccggcatc cggtagcgtt ccgcgaaatc aacagcaaac ttcctgaaca gtaaaatgat
    12841 gtcttcacct ctttcgcgaa ggggcggcac ccgtaccggc accgtattca gcctgtaaaa
    12901 aagatcttcc cggaaccttc ccgagcctac tgcctggaca atatcaacat tggtggcggt
    12961 tatgaccctg acatcggtct tctgaacctt tgatgaacca acccttatga attcacctgt
    13021 ctccagcacc ctgagcagcc tgacctgcgt cgagaggggc agttcagcca cctcgtcaag
    13081 aaatattgat cctccgtttg cgacctcgaa gtagcccttg cgactgtcag ttgccccggt
    13141 gaaagcgccc ttctcgtggc cgaagagttc cgaatcaatc gttccctccg gtatggcgcc
    13201 acagttcacc gcaatgtacg gaccgtgctt gcgcatgctg aatgagtgta tcacctgcgg
    13261 aaagacctcc ttcccggttc cgctttcacc tgttataagt acagagagat ctgtgggggc
    13321 cacctgaacg gctatgtcaa ttgccctgtt cagtccctca tgattgccaa ttattccaaa
    13381 cctctgtttt acgctctgta attccttcat caaaatccaa tgaactgcga atttacgact
    13441 tttttgcatt atgttaaagc tgttttttag taatttggaa ccatgaaaga gagccattca
    13501 ccgcg
//
LOCUS       DAOPXU010000074        13320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_5115_length_13320_cov_12.903053, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000074 DAOPXU010000000
VERSION     DAOPXU010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13320
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5115_length_13320_cov_12.903053"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..3147
                     /locus_tag="PKK03_08395"
     CDS             7..3147
                     /locus_tag="PKK03_08395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX84460.1"
                     /translation="MKKLSLLIGLLVLSVTALIGQTKVITGTVTSAVEGEGALPGVTV
                     QVKGTTIATPTDVNGRYSLNVPENATTLVFSYIGMKKLEVEIAGRSVVDAVLESEMVG
                     LNEVIVSAFGIKRNANEIGSAVTTIENELITQAKATDAASALSGKIAGLQINTVNASV
                     NPDVRVILRGNRSFLGNNQALLVLDGIPVNLAYLSTLNPNDIESMNVLKGGNAAALYG
                     SEAANGVIIVTTKSGSRDKTSIQFSTTTTFDKVSFFPQLQDRFGSGSASDSYGNPLYE
                     AFENQCWGPEFDGSMVQIGLDDENGAKLIVPYSPLPDEKYKFWDTGMTYQNDLSFSSG
                     NETGTIYLAIQDVKINGVVPGDEARRDVFRFNGSKNYRGFTASASINYTIRTTNTSRS
                     GVYWNVLNTPMQIPLTDFSDWSGKYTDAAGNPMTNWADIDHYYNAYYPNPYEELERLR
                     QETRRDYFTGVVTLEQKFTKWLKFEVRGSIAPNWYRYENKNYARTYSPYGLLMYTQYG
                     RSVSRTKNLSAYTVGENMGFRFTTDAILTFDHKFNDISVRAIAGGTTRDSFGKSGEHV
                     AATLEIDDFFNIKNRVGELGGSASWNQNRSMGLFGDITLGYKNYAYLHASGRNDWSSL
                     LDPSQWSFFYPGVDASLVLSELIPGLKGDIISYLKVRGGVSKVGSINIDNYSLENTFG
                     VAGNFPFGSLSAYSVGNTLNNRYLEPEFTLSKEAGVDISFLNSRINTSITAYQTNTTN
                     QTVDMTVSSATGYTSSKINAGEMLNKGIELELKTTPIATPNWRWDFNANYAYWYNRVV
                     SLAGDLEEIDLGNTAIYAIKGMAFPTIKVTQFTRDPQGRVIVDGTTGMPTIDAQTIPR
                     GQTVPKHLIGLQSSLSWKNLSLAISADYRGGHIFRSDLYYDLLFTGVGKLSAINARER
                     FVFPNSVINTGTAENPVYTPNTNVTIQDGGAAWWTSTMRNLSYYSVNSAAVWKIREIS
                     LTYDIPQSVLGFTNNVVKGLTVGVVGRNLFMFLPKNNLYTDPEFGNTTGNAVGRTTSS
                     QTPATRTYGVNLTVSF"
     gene            3175..4833
                     /locus_tag="PKK03_08400"
     CDS             3175..4833
                     /locus_tag="PKK03_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNX84461.1"
                     /translation="MKKLIIILIAVATIGLSGCEKWLDINKNPNDATRANPDLILPGV
                     LKTWGADVQNLTQLYGAWMGYWYHAGGWSGWYSEKKYEITASYLNRFDYYPGQLTDTR
                     FIRLNSGDNVMYPAITDVVDAWYYSRLIDLYGDVPYSEACQPELTLTPKYDDAETIWL
                     DLIDRLDNAITVFNNAVNAADHATNPIYSFKPASDIVFSGDFTKWKQFANTLKLRLVM
                     RLTNVRTAAELKGMMDNTVAHGFITANVSVNPGYSSSDGKTNPWWNTFGKSFSGTVQT
                     SNTQYVLNRYFHEKMAALADPRLEKYFFAPASASPAGTLISRVLGVDGDLVTQPNSTT
                     AANYSWLFIAANATVTSGRVNSGDGALDRAVIMIGPEAYFLQAEAASRGIITTGMTAQ
                     AAYEAGITASMTASKVASADIDPYLTSVNVAWNTAWSATEQIKRIIDQKYIANYFLNH
                     FESFCDYRRTGYPNPMHPTFTNDAALDPLREMLSYYPGGLIRRQIPRLFPYPQAEINI
                     NNDATMAAINAQISKNGVTFNTTSYPFDARTFWDTAPKTIGYDY"
     gene            4854..5783
                     /locus_tag="PKK03_08405"
     CDS             4854..5783
                     /locus_tag="PKK03_08405"
                     /inference="COORDINATES: protein motif:HMM:NF020111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1735 domain-containing protein"
                     /protein_id="HNX84462.1"
                     /translation="MKNISIKLLSLLVLLTMSLTSCLNDLEDFIGDFSSVPAIAEFNE
                     ATNASTGTVGREIIDPTAPAEFSLRLNITVINTLSTDTKITLAIDNALVAAYNTSKGL
                     TGAAAAVPVPAAAITAPTLEVTIPAGQREAEWEFSIDATKVPNPVTTFYILPVKIASV
                     NNDITISGNNYIRLVRILARNEFDGKYKMKGFIMRPGDTGGLEGWFKDQDYSLSTVSG
                     NAVKMNRVQVWANGSNVGGIEAWTIVVDNSGTSPYPITVIDNVQGAAFIMVPGYDSRY
                     DAASKTFFWSVEWGTATPKNRGCTDTLVYVGPR"
     gene            complement(5896..8301)
                     /locus_tag="PKK03_08410"
     CDS             complement(5896..8301)
                     /locus_tag="PKK03_08410"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HNX84463.1"
                     /translation="MRTKGSAFLAFVFITLISLSCSDDFVTVSPPGEWPVIRSYDMDH
                     LCRIALPLGGIGTGTVSLAGNGELKDWRLSGMPLTGSEATGDPENAPFFSIYARTGGS
                     RAVVKALAGPACAEGDQNRGGDSELLHGLPRFANASFDAAFPFGQVHLSDDEMPVDVT
                     IRAFNPMVPGEPEVSGLPVAALYFEVRNKTMDDVIVSVCGTMRNFIGGSAGEPVMNPD
                     VGGIPEGSGQPWNEYIFYRDIQGIYMSSGDADIIDPARRTMALSTAGEDTISWRTGSE
                     ASDLAGAIKEIRDDFSSDGELTELQTGPEGQPFASLAFRDTIGPGRSRYFRFIITWHF
                     PVSKELPGHSNDVNFYTGDFTNAWDVTKKIIPYMPLLEENSVRFVRTLMASKLPQPVL
                     EAALFNLSGLRSPQLFRTADGKFRGWEGSINGDDPCQDYCTTGWNNEQAMPFLFGRLA
                     RSMREIEFDIPGDSFTGTAQSVTEVPGFAAAALPAADFEGQLGAVMRFYRDWQLSGDS
                     LFLTERWGRIKSVITNAWSAGGPDHDRDGVIDRANNGSSDAGRFQADLQTQIWYLGAL
                     RAANEMSKAVNDSDFTGLTDGLFRRGSEWTDDNMLKRTLGRTVSGGQMAGRYMADICG
                     LGNLFRSGTRIKTEGRQPGTESGHEYITAAGMFLSGKPGDGLKILARVQSDHDGLSGN
                     PFGDPDCLCRGVQSMAAWTPVIALTRFRYSAVDESFQIRSSEGVTFWSNGYAWGECVV
                     TGDRGVREAKLKVFGGELRLRKIILDGFGERIISDNPVVIRTGEESVFSVIAAGNGRK
                     GQR"
     gene            complement(8398..>8922)
                     /locus_tag="PKK03_08415"
     CDS             complement(8398..>8922)
                     /locus_tag="PKK03_08415"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNX84464.1"
                     /translation="GAYRIRIRPDVKNLGVYSTNMGSALTEYDGAPVVDFVLDGREVL
                     KNFEFEMPEGEKEIEVAYGTIKRKDLTTDVGYIDGQADENAAYTDIYQLIQGKVPGVQ
                     VNGNKITIRGINSIMLSTDPLFVVDGAVVSTIDNISPRQVKSISVLKGSDAAIYGSRG
                     GGGVILITLVGTKR"
     assembly_gap    8923..9022
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9087..10289
                     /locus_tag="PKK03_08420"
     CDS             9087..10289
                     /locus_tag="PKK03_08420"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNX84465.1"
                     /translation="MTFDELDLDPEILDAIDYMGFRETTPIQEKAIPVILEGHDLIAC
                     AQTGTGKTAAYLLPVMNDIAYHRPAHTHTLILVPTRELALQIDQQIQGLAYTLNITSL
                     AVYGGGEGSAWDQEKAALTHGADIIVATPGRLISHLNLGYVQFTELQVLVLDEADRML
                     DIGFHDDIMKIISFLPKKRQTLMFSATMPPKIRSLSKHIMKNPEEISLEMSKPAEGVL
                     QAVYLLEEPQKLPLLNSLLADKPEYHSILIFSSTKKKVHEIVRGLRSRDYTVEGISSD
                     LEQKEREETLLRFRSRRTRVLVATDVLSRGIDIKDINLVVNFDAPSDAEDYVHRVGRT
                     ARADTTGVAITLVTRADLFKLQRIEKLIGYQVFRAPLPDFISSGHGDTAGHRPSRPRS
                     GNRKFRRR"
     gene            10405..11916
                     /locus_tag="PKK03_08425"
     CDS             10405..11916
                     /locus_tag="PKK03_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNX84466.1"
                     /translation="MTSDEKTMIIIGGGFAGLAAGIYGRLNGYKTEIYEMHSQPGGLC
                     TAWKRNGYTFDACIHWLVGSSPESNMNSMWIETGIAPGRKFVYAGEYYRAEGADGRTI
                     IFHADPDRLEKHLLEAAPQDKETIKDFIAGIRMCSAFDFPSEKEPFLTRIRKHVKTFF
                     TFMKYGKEFRRWTGITGEEFASRFSDPLLKQAFREIWIPEFSMVFLFFTFAYLSKRNA
                     GYPIGGSMPMSEALEKRYRDLGGVIHYNKRVARILTTDGRATGIRLVDGTEASAGRVI
                     SAADGHATLFDMLDGKFLDDKIREQYDTWPVFHSLLFVSLGIKRKFDDMPVSVSGFSF
                     PLNEPVMVADRMRDRLPVHIYNQDPTMAPDGKTSVIIMLESDYAYWKKLAEDRNIYVQ
                     KKDEVAGLIIRLLGQRFPGIGDQVEVTDVATPLTFESFTGNWKGSFEGWLITPKNAYT
                     MMKPMPQTVPGLRNFYMCGQWVEPGGGLPTAIMSARRLIRQICREDGRKFKTE"
     gene            12076..12420
                     /locus_tag="PKK03_08430"
     CDS             12076..12420
                     /locus_tag="PKK03_08430"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNX84467.1"
                     /translation="MLQTDLIKGSLRTIVLKLLKENGRMYGYEITRRVEELTSGKIKL
                     TYGALYPVLHKLESEGVLVTEAENFNNRIRIYYSLTEIGHSLVSEKVRELMEFIESLQ
                     GIVNPKTGPSYA"
     gene            12413..>13320
                     /locus_tag="PKK03_08435"
     CDS             12413..>13320
                     /locus_tag="PKK03_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84468.1"
                     /translation="MPELTFSDIDCIRQDVIRQEICYSHLADELIDHLCCDVENEMAG
                     GLSFRDAYRKVKESLGHRRLREIQEETLYAVDTKYRNMKNTMKISGITGTVMLGFAAL
                     FKIMHWPGAGIMLTLGSLLLAFVFMPSALTVLWKETHSGKKLFLYISAFITAMLLIIG
                     VTAKVQHWPMAGLILAAASLSGTLAFVPALLSAKLKDQENRSKKIVYILGAAGLICSI
                     MGLLCKIQHWPLATILLTFGTAVIFFAVFPLYTWMTWREEKNVSARFIFMIVGLIGLM
                     LPVLMLNLSLQRTFEAGYYIHHEEQQA"
BASE COUNT         3429 a         3496 c         3167 g         3128 t
ORIGIN      
        1 actagtatga agaaactttc actgttgatt ggtttgcttg tattgagtgt gacagcactt
       61 atcgggcaaa cgaaagtcat tacaggcaca gttacctccg ccgttgaggg ggagggcgct
      121 ctgccgggag ttaccgtaca ggttaagggg acaacaattg ccaccccgac cgatgtcaac
      181 ggtagatact cattaaatgt gccggaaaac gcaactactc ttgttttcag ctacatcggc
      241 atgaaaaagc tggaagttga gatcgcagga cggtcagtcg tagacgcagt tcttgaatct
      301 gaaatggtcg gtctgaatga agtgattgta tccgcattcg gtatcaagcg taatgcgaat
      361 gagatcggtt cagccgttac cacgattgag aatgaattga ttacccaggc aaaagccacc
      421 gacgccgcat cggccctgtc aggtaagatt gccggccttc agatcaatac cgtgaacgcc
      481 agtgttaacc ctgatgtcag ggtcatcctc aggggtaacc gttcattcct gggcaataac
      541 caggctctgc tcgtccttga cggcatcccc gttaaccttg cctacctctc aaccctgaac
      601 ccgaatgaca ttgaaagcat gaacgtcctc aagggcggta acgctgctgc tctttacggt
      661 tcagaagccg caaacggtgt tatcatcgtg acaaccaaat caggatcgcg tgacaagacc
      721 tccatccagt tcagcacaac aacaaccttt gacaaagtat cattcttccc gcagctgcag
      781 gatcgctttg gctcaggttc agcatctgac agctacggca acccgcttta cgaagctttc
      841 gaaaaccagt gctggggacc ggagtttgac ggatcaatgg ttcagatagg tctcgatgat
      901 gagaatggcg caaaactgat agtaccctat tcaccgctcc cggatgagaa gtacaagttc
      961 tgggataccg gcatgaccta tcagaatgac ctttcattct cttcgggaaa tgagacaggt
     1021 accatctacc ttgccataca ggatgtaaaa atcaatggcg ttgtccccgg agatgaagcc
     1081 cgccgtgacg tgttccgttt caacggatca aagaattaca gaggctttac agccagtgca
     1141 agcatcaact acaccatccg tacaaccaat acctcaaggt caggtgtcta ctggaacgtt
     1201 ctgaacaccc ccatgcagat tcctcttacc gacttcagcg actggagcgg caaatacact
     1261 gacgcggcag gaaatcctat gacaaactgg gcagacatag accattatta taatgcatat
     1321 taccccaacc cctacgaaga gcttgaaagg ctgaggcagg agacaaggag agactacttt
     1381 accggtgtcg ttacccttga acaaaaattc acgaagtggc tcaagttcga ggtcagaggt
     1441 tcaatagccc caaactggta tcgttacgag aataagaact atgcccgtac ctacagtccg
     1501 tacggacttc tcatgtacac acagtatggc cgcagcgtaa gccgtaccaa aaacctgtca
     1561 gcctataccg ttggtgaaaa catgggcttc aggttcacca ccgatgctat ccttacattt
     1621 gatcacaagt tcaatgacat ttcagtaagg gctatcgccg gcggaaccac cagggacagc
     1681 tttggcaaga gcggtgagca tgttgctgcc actctcgaaa tcgatgactt cttcaatatc
     1741 aagaaccgtg ttggggaact tggcggatca gccagctgga accagaacag gagcatgggt
     1801 ctctttggag acataaccct tggttacaag aactatgctt acctgcatgc ttcaggcaga
     1861 aatgactggt cgagccttct tgatcccagc cagtggtcat tcttctaccc gggcgttgat
     1921 gcatcacttg tcctttcaga gcttattccg ggcctcaagg gtgatatcat cagctacctg
     1981 aaagtcagag gaggtgtatc aaaggttggt tctatcaaca ttgacaacta ctcacttgag
     2041 aatactttcg gtgttgcagg caacttcccc ttcggaagcc tttcagcata cagtgttggt
     2101 aacaccctca acaacaggta tcttgagcct gaattcaccc tctcaaaaga agccggggtg
     2161 gacatcagct tcctgaacag ccgtatcaac acaagcatca ctgcctacca gacgaacact
     2221 accaaccaga cagttgatat gacggtttca agcgcaaccg gttacaccag ctccaagatc
     2281 aatgccggtg agatgcttaa caagggtatt gagcttgaac tcaagacaac cccgatagct
     2341 actcccaact ggagatggga tttcaatgcc aactatgcat actggtataa cagagttgta
     2401 tcacttgcag gcgaccttga agaaatcgac ctcggcaaca ctgccatata tgctatcaag
     2461 ggcatggcct tcccgacaat caaggttacc cagtttacac gtgacccgca gggaagagtc
     2521 atagttgacg gaacaaccgg catgcctacg atcgatgcac agaccatccc gagaggccag
     2581 acggttccca agcaccttat cggacttcag tcaagcctca gctggaagaa cctttcgctc
     2641 gcaatcagtg ctgattatcg cggcggacat atcttccgtt ccgacctgta ttatgacctt
     2701 ctctttaccg gtgtcggcaa actttctgcc atcaacgcac gtgaaagatt cgtattcccc
     2761 aattcagtca taaatacagg gaccgcagaa aacccggttt acactcccaa tacgaatgtt
     2821 acaattcagg acggtggcgc cgcatggtgg acaagcacga tgagaaacct cagctactac
     2881 tcagtgaaca gcgctgctgt atggaagatc cgcgaaatat cactcacata tgatattcct
     2941 cagtcagtac tgggctttac gaataacgtc gttaaaggac tcacagttgg tgttgtcggt
     3001 agaaacctgt tcatgttcct gccgaagaac aacctctaca cagacccgga atttggcaac
     3061 accacaggta acgctgtcgg tcggacaacc agttctcaga ctcctgcaac aaggacttac
     3121 ggtgtcaacc tgactgtatc cttctaataa cccattcaaa atttcagaaa gaacatgaaa
     3181 aagttaataa taatcttaat agctgtagcg actatcgggt tgtcaggttg tgaaaaatgg
     3241 cttgacataa acaaaaaccc caacgatgct accagagcca atcccgacct gattctgccg
     3301 ggagttctca agacatgggg agctgatgtt cagaacctca cccaacttta tggagcatgg
     3361 atgggttact ggtaccatgc aggcggatgg tcaggatggt acagtgaaaa gaaatacgag
     3421 atcactgcca gttacctgaa ccgtttcgac tactacccgg gccagctgac tgacaccagg
     3481 tttatccggt tgaattcagg tgacaatgta atgtatcctg caattacaga tgttgttgat
     3541 gcatggtatt attcacgtct gatagacctt tacggagatg ttccttactc cgaggcatgt
     3601 cagcctgaac tgactcttac tccaaagtat gacgatgcag aaaccatctg gcttgacctg
     3661 atcgacaggc tcgataatgc aattaccgta ttcaacaatg cagtgaatgc agctgaccat
     3721 gcaacaaatc cgatctatag tttcaagcct gccagtgaca tagtcttctc aggtgatttt
     3781 accaaatgga agcaatttgc caatactctt aagctgaggc tggtaatgag gctcaccaac
     3841 gtcaggactg ccgcagaact caagggaatg atggacaaca ccgttgctca cggctttatc
     3901 acggccaacg ttagtgtcaa cccgggctat tcgtcatcag acggtaagac caacccctgg
     3961 tggaacacct ttggcaagag cttcagtggc accgttcaga cctccaacac ccagtatgtc
     4021 ctgaacaggt acttccacga gaagatggca gcacttgctg atccgcgcct tgaaaagtat
     4081 ttctttgcac ctgcatcggc ttctcctgcc ggtaccctca tttcaagggt tctcggcgtt
     4141 gacggcgacc tggtcaccca gcccaactca accaccgcag ccaactattc atggctcttc
     4201 atagcagcga atgctactgt gacaagcggc agggtcaaca gtggtgacgg cgctcttgac
     4261 agggctgtca tcatgatagg tcctgaggca tacttcctcc aggctgaagc cgcttcccgt
     4321 ggtattatta ccaccggcat gactgcacag gcagcctacg aggcaggtat aacagcttca
     4381 atgactgctt caaaagttgc ttcagctgac atagacccgt acctcaccag tgtaaacgtg
     4441 gcatggaaca cagcatggtc agccaccgaa cagataaaaa ggatcattga ccagaagtat
     4501 atagcaaact acttcctgaa ccatttcgaa tcattctgtg actaccgtcg tacaggatat
     4561 ccgaacccga tgcacccgac attcaccaat gacgcggctc ttgacccgct tagggagatg
     4621 ctgagctact atccgggtgg tctcatcagg aggcagatac cgaggctgtt cccgtatccg
     4681 caggctgaaa tcaacattaa taatgatgct acaatggctg ccatcaatgc tcagatttca
     4741 aagaatggcg ttacattcaa caccacttcc tacccgtttg acgccaggac attctgggat
     4801 actgcaccca agacaatcgg atacgattat taaatcaata aaaactactg acaatgaaaa
     4861 atataagtat aaaactgtta tcgcttctgg tccttctgac gatgtctctc acatcatgtc
     4921 tgaatgacct cgaagatttc attggtgatt tcagcagtgt tccagcgata gccgaattca
     4981 atgaagcaac gaatgcttcc acaggtaccg tgggaaggga gattatcgac ccgaccgcac
     5041 ctgcagagtt ctctctccgt ctgaacatca ctgtaatcaa cactctcagc accgatacaa
     5101 agatcaccct tgctatcgac aatgccctgg tggcagctta caacacctcc aaagggctta
     5161 cgggtgcagc tgctgccgta ccggttcctg cagctgctat cactgctccc acacttgagg
     5221 tgaccatacc tgcaggacag agggaggcag aatgggaatt ttcaattgat gccacaaaag
     5281 ttcccaatcc tgtgaccacc ttctacatcc ttcctgtgaa gattgcctcc gtgaataatg
     5341 acattacaat cagcggaaac aattacatca ggctcgtgag aatcctggca aggaatgagt
     5401 tcgacggcaa atacaagatg aaaggcttca tcatgagacc cggtgacacc ggaggcctcg
     5461 aaggctggtt caaggatcag gattacagtc tcagcactgt cagcggcaac gcagtcaaga
     5521 tgaacagggt tcaggtatgg gctaacggct caaacgttgg cggtatcgaa gcctggacta
     5581 tcgtcgttga caactctggc acctcgccct atccgatcac tgttattgat aatgtccagg
     5641 gagcagcatt catcatggtt ccgggctatg acagccgcta tgatgctgca tccaagacat
     5701 tcttctggag cgttgaatgg ggtacggcaa ctcccaagaa caggggatgc acagataccc
     5761 ttgtttacgt aggcccaaga taatggcttg acaattttga ctataagaga ggctgcctca
     5821 gaagggcagc ctcttttttg tctccgtctg cagatacaca gctgtcagat tatcctatgt
     5881 ctcatacggt accggtcacc gctgaccctt tctaccattc ccggcggcga tcactgaaaa
     5941 tactgactcc tccccggttc ttatcacaac cggattatct gaaatgatac gttcgccaaa
     6001 tccgtcgagg atgatctttc ttaaccgcag ctcacctccg aaaactttta atttagcctc
     6061 ccggactccc ctgtcgcctg tcacaacaca ttcgccccag gcgtagccgt ttgaccagaa
     6121 ggtgactcct tctgaagacc ttatctgaaa tgactcatcc accgcggaat aacggaaccg
     6181 ggtgagtgca atcactgggg tccatgctgc cattgactgc accccccggc acagacaatc
     6241 gggatcgccg aaaggatttc cactgagacc gtcatggtct gattgcactc tggctagaat
     6301 cttcagtccg tccccgggct ttccgctcag gaacataccg gcagctgtaa tgtattcatg
     6361 gccggactcc gtaccgggtt gcctgccttc agtttttatc cgcgtaccgc tcctgaaaag
     6421 attgcccagg ccgcagatat cagccatgta ccggccggcc atctgaccgc cagaaacagt
     6481 tctgccgagc gtccgtttca acatgttatc gtctgtccat tccgagcccc gcctgaacag
     6541 cccgtcagtc aatccggtga aatcactgtc attcacagcc tttgacatct cattcgcagc
     6601 cctcagggcc ccgaggtacc atatctgtgt ctgtaagtca gcctgaaatc ttccggcatc
     6661 agaagatcca ttgttggctc tgtcaatcac tccgtccctg tcatggtccg gtccgcctgc
     6721 agaccaggcg ttggtaatga ctgactttat cctgccccag cgctccgtaa ggaacaggct
     6781 gtctcctgat aattgccagt cacggtaaaa tctcattaca gcacccagtt gcccctcaaa
     6841 gtcagcagca ggaagggccg ctgctgcaaa gcccggcact tctgttaccg actgtgcagt
     6901 tcctgtaaag ctgtcacctg gaatgtcaaa ttcaatttct ctcattgatc tggcaagcct
     6961 gccaaataga aaaggcattg cctgttcatt gttccatcca gtggtgcagt aatcctggca
     7021 ggggtcatca ccattgatgg atccttccca gccgcggaac ttcccgtcag ccgtacggaa
     7081 cagctgcgga gaccgcaggc cgctcaggtt gaaaagtgcg gcttccagaa ccggctgagg
     7141 aagtttgctt gccatcagcg ttcgcacaaa cctgaccgag ttctcctcaa gtaaaggcat
     7201 gtacgggatt attttctttg tgacatccca tgcattggtg aaatctcctg tatagaaatt
     7261 aacgtcattg ctatgacccg gcagttcttt actgacggga aaatgccatg taattatgaa
     7321 cctgaaatac ctgctccggc ccggccctat cgtatccctg aaagccagtg atgcaaatgg
     7381 ctgaccttca ggcccagtct gcaactcagt gagttcaccg tcactgctga agtcatcgcg
     7441 gatctccttt attgcgccag ccaggtcaga agcttcagat ccggtccgcc atgatattgt
     7501 atcctctccc gctgttgaga gagccatggt gcgccgtgca ggatcaatga tgtcagcgtc
     7561 gccggaagac atatatattc cctggatatc cctgtaaaag atatattcat tccagggctg
     7621 gccggaccct tcaggtattc cacctacgtc gggattcatc accggctcgc ccgcgctacc
     7681 accaatgaag tttctcatgg tgccgcagac tgacactatc acgtcgtcca tggttttgtt
     7741 cctgacttcg aaataaaggg ccgcaacagg cagtccgcta acctccggtt cacccgggac
     7801 catcgggttg aatgccctga ttgtcacatc caccggcatc tcatcgtcag agagatgcac
     7861 ctgtccaaag gggaaggctg catcaaacga agcattggca aaccgtggca gaccatgcag
     7921 cagttcagag tctccgcccc tgttttgatc tccctccgcg catgccggtc cagccagggc
     7981 cttgacaaca gctcttgaac cacctgtcct ggcataaatt gaaaagaagg gagcattctc
     8041 cgggtctcct gttgcctcag atcctgtgag aggcatgccg gaaagtctcc agtccttcag
     8101 ttcgccgttc ccggccaggg agaccgtccc tgttcctatt cctcccagag ggagggcaat
     8161 ccggcacagg tgatccatgt catatgacct gattaccggc cactcaccgg gcggggagac
     8221 agtcacgaag tcgtcagaac aggaaaggga gatcagtgtt ataaaaacaa atgccaaaaa
     8281 tgctgagcct ttggttctca taaagaaaga tgttgatcca cagattaaaa cttgtaataa
     8341 caggtgatat gcagtccgct ttcaggccat tatcgtcccg tgaggcagca gcatccgcta
     8401 cctctttgtt ccgacaaggg ttatcaggat gacaccgccg ccaccccttg agccgtaaat
     8461 tgcagcgtca gagcccttca gcacgctgat cgacttgacc tggcggggac tgatattatc
     8521 tattgtactg accacagcgc catcaaccac gaaaagcggg tcggtgctga gcataatgga
     8581 gtttattccc ctgattgtga tcttgttgcc attcacctgt actcccggca ccttaccctg
     8641 gataagctga taaatatctg tatatgccgc gttctcatca gcctgtccat caatgtaacc
     8701 cacatcggtt gtcagatcct tcctcttgat tgtcccgtat gcaacctcga tttccttttc
     8761 tccttcaggc atttcaaact cgaaattctt tagtacttcc ctgccgtcaa gaacaaaatc
     8821 aaccacgggc gctccatcat attccgtcaa tgcagatccc atattcgtgc tgtatacacc
     8881 gaggttcttc acatccggcc tgatacgtat cctgtaggcg ccnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnagttttcg atcttttttt actacttttg ctgccctcca
     9061 cactggagat tattaatcaa atatttatga cttttgacga gcttgatctg gaccccgaaa
     9121 ttcttgatgc aattgactac atgggattca gggagaccac ccctatacag gagaaggcga
     9181 tcccggtgat ccttgaaggg catgatctta ttgcctgtgc acagacaggc accgggaaga
     9241 cggctgctta cctcctgcct gtgatgaatg acattgcata tcaccggcct gcacataccc
     9301 acacccttat acttgttccg acacgtgagc tggcccttca gattgaccag cagatacagg
     9361 gtctggcata cacgcttaat attacctcac tggccgttta cggaggaggt gaggggagcg
     9421 catgggacca ggagaaagct gcgctgacac acggggctga cattattgtg gcgactccgg
     9481 gaaggcttat atcacatctc aacctcggtt atgtacaatt cacggagttg caggtgcttg
     9541 tgcttgacga agcagacagg atgcttgaca taggtttcca tgatgacatc atgaagatca
     9601 tttccttcct tccgaaaaag cgtcagacac tgatgttcag cgccacaatg ccgccaaaaa
     9661 taaggtcgct ttccaaacat ataatgaaga accctgagga gatctccctg gagatgtcga
     9721 agccggccga gggggtgctc caggctgttt acctgcttga ggagccgcag aaactccccc
     9781 tgctcaacag cctcctggcg gacaagcccg aataccacag catcctcatc ttcagctcaa
     9841 caaagaagaa ggtgcatgag attgtcaggg ggctccgctc acgtgactac actgtggaag
     9901 gcatctcatc agaccttgaa cagaaggaac gtgaggagac tctgctgcgg ttccgctcca
     9961 ggcggacgcg tgtactggtt gccaccgatg tgctgagccg gggtatcgat ataaaggaca
    10021 tcaacctggt tgtcaatttc gacgcaccct cagacgcgga ggactatgtg caccgtgtgg
    10081 gccgtacggc tcgtgctgac accacagggg tggcaataac cctggtaacc agggctgacc
    10141 tgttcaagct ccagagaatt gaaaaactga tcggttacca ggtgttccgt gcaccgctgc
    10201 ccgacttcat ctcttcaggt cacggtgaca ctgcgggcca tcgcccgtcg cgtccccgct
    10261 ccgggaacag gaaattcagg cgccgctgaa atctttcgcg gtatcgcgga agaaccccac
    10321 gttctgtttt aaaaaccggg cagggaacca ttgttgatta tctgattgtt ctataacttt
    10381 gatctccact aatccttttt cccgatgaca tcagacgaaa aaacaatgat cattatcggc
    10441 ggaggctttg ccggcctcgc cgcaggtatt tacggacgcc tcaacggcta taaaacagag
    10501 atctacgaga tgcacagtca gccgggcgga ctgtgtacag catggaagag aaacggctac
    10561 accttcgacg cctgcattca ctggctggtg ggctcctcgc ctgagagcaa catgaatagc
    10621 atgtggatcg agacaggtat cgcaccagga aggaaattcg tttacgccgg cgaatactac
    10681 cgcgccgagg gggctgacgg gagaacaatt atcttccatg ctgatcctga caggcttgag
    10741 aagcatctcc ttgaggctgc ccctcaggat aaggaaacca taaaggattt catcgcaggg
    10801 ataagaatgt gttctgcatt tgacttccct tccgaaaaag aaccgttcct gacacggatc
    10861 aggaaacatg tcaaaacctt ctttacgttc atgaagtacg gaaaagagtt ccgcaggtgg
    10921 accggcatta ccggtgagga gtttgcttcg cgcttcagcg atcccctcct gaagcaggca
    10981 ttccgcgaga tatggatccc ggaattctcg atggtcttcc tctttttcac tttcgcttac
    11041 ctaagcaagc gcaacgccgg gtacccgata ggcgggtcaa tgccaatgtc ggaagccctt
    11101 gaaaaacgat acagggacct tggcggagtg atacactata ataaaagggt ggcgaggata
    11161 ctcaccactg acggccgggc taccggaata aggctcgttg acggcaccga ggcaagtgcc
    11221 ggcagggtca tctctgcagc tgacgggcat gccacactgt ttgatatgct tgacggaaag
    11281 ttccttgatg acaagataag ggagcaatat gatacatggc cggtgtttca ttcactcctg
    11341 ttcgtttcgc ttggaataaa aaggaagttt gatgatatgc cggtcagtgt ctccggattc
    11401 tccttcccgc tgaatgagcc ggtaatggtt gcagacagga tgagagacag gcttcctgtg
    11461 catatctata accaggatcc gaccatggct cctgacggga agacatcagt gataataatg
    11521 ctggaatcag actacgcata ctggaagaaa ctggccgaag accggaatat ctatgttcag
    11581 aagaaggatg aggtggccgg gctgataatc aggctgctcg ggcagcgatt cccggggatc
    11641 ggggaccagg tggaggttac cgatgtagca actccgctta cgttcgagag cttcaccgga
    11701 aactggaagg gttcattcga gggatggctc atcaccccga agaatgctta cacgatgatg
    11761 aagccgatgc ctcagacagt gccggggctg cgaaatttct acatgtgcgg ccagtgggtt
    11821 gaaccgggag gcgggctgcc caccgccatc atgtcagccc ggaggcttat caggcagatc
    11881 tgcagggaag acggcaggaa attcaagact gaataatccc ggccgacagc tgccggcatt
    11941 cgctatgggc gctatgaagc cccgtcaaaa gcaatcgcca tagtcatttg cgaagtgatc
    12001 accaaaaata tatttgcatt tgtacaataa aatattgtat ttttacatta tataaacatc
    12061 ttatatatta tcaatatgct tcaaactgat cttatcaaag gttctctccg gacaatcgtt
    12121 ctcaagctgc tgaaggaaaa cggccgcatg tatgggtatg agataacccg cagggttgag
    12181 gaactcacct ccggaaaaat caagcttacg tacggtgcac tttatcccgt gctccataag
    12241 ctcgaaagtg agggagtact ggtcacggag gcagaaaatt tcaacaaccg catccggatc
    12301 tattacagcc tgacggagat aggccattca ctggtcagtg aaaaggtcag ggaactgatg
    12361 gagttcatag aatctctcca gggcattgtt aacccgaaga ctggtccgag ctatgcctga
    12421 gctgactttc agtgatattg attgcatcag gcaggatgtg attcgtcagg aaatctgcta
    12481 ttcgcacctt gctgacgagc tcatcgatca tctctgctgc gatgtggaga atgagatggc
    12541 cggtggtctc agcttccgcg acgcatacag gaaggtgaag gaatcacttg ggcaccgcag
    12601 gctcagggag atacaggaag aaactcttta tgctgtagac accaaataca ggaatatgaa
    12661 aaacacaatg aaaatttcgg gtatcaccgg cactgtcatg cttggatttg ctgccctgtt
    12721 caagatcatg cactggcccg gcgccggtat catgcttacc ctcggcagcc ttttactggc
    12781 ttttgtcttc atgccatcgg cactgacggt tctctggaaa gagacacaca gcgggaagaa
    12841 actcttcctg tatatatcgg cattcatcac cgcaatgctc ttaataatag gtgtaaccgc
    12901 aaaggtacag cactggccga tggccggact gatcctggct gctgcatcac tgtcagggac
    12961 ccttgccttt gtccctgccc tgctctctgc aaaactgaag gatcaggaaa acaggtcgaa
    13021 aaagatagtc tatatcttag gtgctgcagg cttgatctgt tccatcatgg gattgctctg
    13081 caagattcag cactggccac tggcaaccat acttctgaca ttcgggactg cagtcatatt
    13141 cttcgcagta tttccattgt acacctggat gacctggagg gaagaaaaaa acgtcagcgc
    13201 cagattcatt ttcatgatag tcggcctgat aggtctgatg ttgccggttt taatgctcaa
    13261 cctcagcctg caaaggactt ttgaggcagg ttactacata catcatgaag aacaacaggc
//
LOCUS       DAOPXU010000075        13165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_5207_length_13165_cov_11.474828, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000075 DAOPXU010000000
VERSION     DAOPXU010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13165
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5207_length_13165_cov_11.474828"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="PKK03_08440"
     CDS             complement(<1..1406)
                     /locus_tag="PKK03_08440"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNX84469.1"
                     /translation="MNKINLIFMVMLSASMVSVPEAHGCTIIAAGKKATADGSVLISH
                     TDTGPDSRIYYVPGKTFRAGEQAPVYWGIQDAGLSLMDDGEVLGYIPQVRKTYGYFHS
                     AYSHMNEVQLGIAESTTAQRPELVCTREEGKQIMTIEQAMIFALQRCDKAREAVLLIG
                     DLMIRYGFLPSSGDGSETLVIADTDEAWVLEVFGVGNGWEPGTGRPGAIWAAQRLPDD
                     QATMVPNWSIIKEINAGDKSRFLVSDNYMKEAVDRGWYDPASGKPFIWQEAYSPLPEE
                     YATSRFWLFYSTFMPGLKQWPDRKLDPSNPYKGMQPYYQVVEPLSIYPFAAVPEKKIS
                     VQDVIAFQRSTFEGTIYDMTSYPEWLVPDGKGGYEKSPLATPFPGSEMRKLIKQTYRR
                     PVARHRGHYGMVMQLRDWLPDAIGGVYWVYLDNPYFSPYVPIYAGNLSVAETYNIYDP
                     EKYDERSARWAIDFVDNLA"
     gene            1543..1725
                     /locus_tag="PKK03_08445"
     CDS             1543..1725
                     /locus_tag="PKK03_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84470.1"
                     /translation="MKKLIFLVLAILMLASCNQGVCPAYSDSANDMKNTLLQKGNIRA
                     DGINRVYRDSYQKKLR"
     gene            complement(1776..2174)
                     /locus_tag="PKK03_08450"
     CDS             complement(1776..2174)
                     /locus_tag="PKK03_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84471.1"
                     /translation="MTIRKVILLFIFVLVASCSTSNRAATEPEEDRLYVTRMYIGDYQ
                     DFRHTAPARFGDPHLIWIKTSADSTFGKISAYSRECGFNEGDRLYLRRQYVTPGIYGY
                     WMYQIENDSSVFYRVCEYQNDKKVLVQSWY"
     gene            complement(2249..2383)
                     /locus_tag="PKK03_08455"
     CDS             complement(2249..2383)
                     /locus_tag="PKK03_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84472.1"
                     /translation="MKTYYQISSDVTGKVLLRRRKIAKALRWWLNENGYTYKYLFYSV
                     "
     gene            complement(2411..2890)
                     /locus_tag="PKK03_08460"
     CDS             complement(2411..2890)
                     /locus_tag="PKK03_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84473.1"
                     /translation="MKSTAVFKIVSMIILCGFSFFRYMRRTGLFGGPGLIILSSHEGA
                     NYGRTDLSRARTLISLRRLDLIKHLEMFVSSLVRLLPPETSFVGCFAYRRSSGLDAQH
                     PGNMPLYLSRIIHHRSAVREELNPDRVSAILGRCGLVVIDMKELNGLTYFHSRKRIN"
     gene            complement(3152..3613)
                     /locus_tag="PKK03_08465"
     CDS             complement(3152..3613)
                     /locus_tag="PKK03_08465"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaC family regulatory phosphoprotein"
                     /protein_id="HNX84474.1"
                     /translation="MEKPTDLIIGKTPKTPQIELKYSSGELSLTGRSLPENATRIYEP
                     VLNWVNNYVIQPQPVTNLRLNVEYFNTSSSLWISKIIKALTRINNPDYRLIVHIYIPL
                     EEYDDMKLSADVRDSFNPITDILQDSIPEVSLKLYATDDTSRVIRDALVLF"
     gene            complement(3807..4442)
                     /locus_tag="PKK03_08470"
     CDS             complement(3807..4442)
                     /locus_tag="PKK03_08470"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HNX84475.1"
                     /translation="MSKKKDAACCRYRSHPWHGIDIGNKAPEIVMTYVEMVPTDTVKY
                     EMDKISGYLRLDRPQRYSNVVPALYGFIPRTLTGADVADRTMEKTGLAGIKGDGDAMD
                     ICILTEKDITHGDILVLARPIGGFRMIDGNEADDKIIAVLYNDAVYGEYADIMDCPQI
                     AIERLKHYFLTYKDLPGEKRRVTIADIYDTAEAQEVIRRSMSDYAREFPGM"
     gene            complement(4472..5275)
                     /locus_tag="PKK03_08475"
     CDS             complement(4472..5275)
                     /locus_tag="PKK03_08475"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNX84476.1"
                     /translation="MQKTVRAFLIVALVVAACTRLPDPEPVDNPRVVILMYHRITEGD
                     AGNLYERSAEEFEKDLLYLQENNIRVIDFNDLAAVISGEQELTTHAALITFDDGDHSW
                     YTLAAPLLKRYRMKATFFLWASKIGMDSFLDWDEVERLSSYTFDGGVRPFTFGSHTMS
                     HQYLYNMKSALGGGEAFQAYLDEELGGSKVLIDRHIYGIVEALALPYGDGAGDEDIIA
                     GARRHGYSFIRTSERNVTGMAGADLFRLPSLPLLDDTDQEIIGTYLGIQ"
     gene            complement(5372..7387)
                     /gene="uvrB"
                     /locus_tag="PKK03_08480"
     CDS             complement(5372..7387)
                     /gene="uvrB"
                     /locus_tag="PKK03_08480"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNX84477.1"
                     /translation="MEFRLTSDYTPAGDQPEAIRQLAGGLIQGVPYQTLLGVTGSGKT
                     FTIANVIAQLNRPVLVLSHNKTLAAQLYGEFREFFPENAVEYFVSYYDYYQPEAYLPV
                     TDTYIEKDLSINDEIEKLRLSATSSLLSGRRDVIVVSSVSCIYGIGNPDDFRSNTVHV
                     IRGAKISRTLLLRRLVDSLYARNELEFSHGTFRVRGDTVDVFPAYADLALRIVFFGDE
                     IEEVSTFDPLSGQHRDDLDEYVIYPANLFVTTRDRINTAVSLIQDDMVLQARHFRDTG
                     RPEEAKRIEQRVQYDLEMIKELGYCSGIENYSRYFDGRAPGTRPFCLLDCFPKDFITV
                     IDESHVTVPQIRAMYGGDHSRKQTLVEYGFRLPSALDNRPLRLEEFEALTGQTIYMSA
                     TPAEYEMEKSEGVFVDQVIRPTGLLDPPVEVRPSRNQVDDLMEEIRKRSARNERVLVT
                     TMTKRMAEELNNYLARFDIKSRYIHSDVETIERVEILEGLRSGVFDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRSGRALTQTAGRAARNINGLVVMYADRITGSMQETINET
                     KRRQARQLKYNSEMGITPQQVVKKSGTMLKGLSHKGVELHAYTGPVKVDVAADPVVKY
                     MSREQLEKAIDKTRKAMEKAAAALDFIEAARLRDEIEGMRKLANERG"
     gene            7522..10575
                     /locus_tag="PKK03_08485"
     CDS             7522..10575
                     /locus_tag="PKK03_08485"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNX84478.1"
                     /translation="MCHPCRTLVFLFTSLLLAGPGTDASCQGYVLTHYNSRNGIGHDN
                     VRTIATDSSGFIWLATWDGLTRYDGTDFVSYFHDPADTATIPYFSVSQVSIDKEDNLW
                     ISTDNGQLSLFNRTTEKFSRVRSLGGYPVSDLTCFNPGPDGFMWFILGKGVLKYNPLS
                     KETLFFPWRKNPGDSLQLVFGSTRLLFDENKEPWLTRNDVLKLNLVTDQAAGDRYAAI
                     NSFIPIERLPGRIGTFFDEVGYAKIVHDTPGNLWLASMTGLFRYDHARNRFIEYSGDF
                     RKIRFAGDQPVIFYNHDTGLNISFPDRDTLFTIPRETCGLPTTCLMTDPGLLWFSHSW
                     QGGTSAGVVKAVFTPHEFRFINPFREKSGELNVFGLAKDEQGALWIAARDRNYTIRID
                     SAGVPERKFLLNDREITELWHPRSFLPDTGSMWIGYFFKRLVHYDLATGKPEYHYPGK
                     SIHTTCYDDQGRILFADDGIMRYDPVTRTTEQLLATGDTMYIFTFHRRGDTLWAGCSH
                     SYLLRYNLSTGEHSPVKILEGMTNVEDICDGDDGTLWIATLGTGVCHYNPYTGEKLFY
                     TTAAGLSNNTTYCILRDRNGNIWVSTNDGLSVINPVTGLIRSFGENDGLMIHEFNSDA
                     SWVNEDGTFILGGVGGAVEFDPAEVLSEPKGMPKQQIIITELEVSALKRVLDEPVYMT
                     DTVVLRKGDDNFHLSFVVPEYRNPDMTRFRYRLGGDEANWYYTDHNDRNINFSNLSPG
                     WYDLHIEATDINGSWSNSRLLTIRVKPYFYQTILFKITLPLFLLTLFSFIAWSVLKQM
                     NQRERQKRDMLRQQALRAQMNPHFIFNALNSINYFISNNDRLSANRYISDFSKHIRTV
                     LNNMNEDFVRLSVEIDALEDYLKIEHLRFGDKFDYAIKVDQDVKAESFRISPGLIQPF
                     VENAIWHGVMGLSGRKGMITVAVKMNGSGLLCMVEDDGVGRARSEAMKDGTLPKKSRG
                     ISLATERMKIINNLLLTNYRITISDLYPDRTETGTRVEIELPLAP"
     gene            10627..11370
                     /locus_tag="PKK03_08490"
     CDS             10627..11370
                     /locus_tag="PKK03_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNX84479.1"
                     /translation="MIRAVIIDDEKLARDVISNYLQDYCPEVEVVAMASSVKTALTAI
                     QKTTPDLVFLDIEMPDGTGFDLLNMFEKIEFKIIFVTAYSEYAIKAFRFSALDYLLKP
                     VKIDELIDAVARVRSAATPGISSEIISSLLENLRSNSPRQSTLIIPNIKGFEVLRVAD
                     IIMCQADGYCTNFLLSGNRKVVSSRNLKHFDGLLEDQSFLRVHHSYLINLEHVTGYTR
                     QGEIILSEGLKAFLGDSYKNEFVRRFTGK"
     gene            complement(11690..12550)
                     /locus_tag="PKK03_08495"
     CDS             complement(11690..12550)
                     /locus_tag="PKK03_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNX84480.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGKEELISVEEEVELAQRIK
                     KGDRAALEKLTKANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAFARFEQENERIPSPEELAE
                     VLDLPKEKVTDTLKVSGRHVSVDAPFVEGEDNSLLDVLTNVDSPIADKMLIDESLSKE
                     IDRALATLTERERDIIRYFFGIACQEMTLEEIGEKFGLTRERVRQIKEKAIRRLRHTS
                     RSKLLKGYLG"
     gene            complement(12692..>13165)
                     /locus_tag="PKK03_08500"
     CDS             complement(12692..>13165)
                     /locus_tag="PKK03_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNX84481.1"
                     /translation="GLAEKDVIIAINGEPVETTADLQEKVSRYRPGDRLEVTYLRKGR
                     QDKKNVVLRNIEGGTGVVAPGAQSSSVFGATFTPVTAGEKSQFKISGGVKITSISDGR
                     FRDLGLSKGTVIIDVNGVKVNSGSDVRKATGDEKSLTSVEGFTPDGTYFKYQTRR"
BASE COUNT         3217 a         3483 c         3093 g         3372 t
ORIGIN      
        1 gcaaggttat cgacgaaatc tatcgcccac cgggcagagc gttcgtcata cttctccgga
       61 tcgtagatgt tgtaggtctc tgccactgaa aggttcccgg cgtagatcgg aacatacgga
      121 ctgaaataag ggttgtcgag atacacccag tacacgccgc caatggcatc cggaagccag
      181 tcgcgcagct gcatcaccat gccgtagtga ccacggtgac gggccaccgg ccttctgtag
      241 gtctgtttga tgagcttccg catctcactc cccgggaatg gggtagccag cgggctcttc
      301 tcataccctc cctttccgtc cggcaccagc cactcaggat aggaagtcat gtcataaatg
      361 gtaccctcga aggtcgatct ctggaaagca atcacatcct gcactgaaat cttcttctcc
      421 gggacagctg caaacgggta gatggagaga ggctccacca cctggtagta gggctgcatc
      481 cccttgtagg gattggacgg gtcaagcttc ctgtcaggcc actgcttcag gccaggcata
      541 aaggtgctgt agaaaagcca gaaacggctg gtggcgtatt cctcaggcag cggggagtag
      601 gcttcctgcc agataaaggg tttccccgac gcagggtcat accagccgcg gtcaactgcc
      661 tctttcatat agttgtcaga gaccaggaac cggctcttgt ctccggcatt gatctccttg
      721 ataatactcc agtttggcac cattgtcgcc tgatcatcag gaagccgctg tgctgcccag
      781 atggccccgg gcctgccggt cccgggttcc cagccgttgc ctactccgaa tacctctaag
      841 acccatgcct catctgtgtc agcaataacc agcgtctcag aaccgtcgcc acttgacggc
      901 aggaagccgt aacggatcat caggtccccg ataagcagca ctgcttccct tgctttgtca
      961 caccgctgaa gggcaaagat catagcctgc tctattgtca ttatctgctt cccctcctca
     1021 cgggtacaca ccagctcagg ccgctgtgcc gtggtgctct cggcaatgcc cagttgcacc
     1081 tcattcatgt gcgagtaggc tgaatggaaa taaccatagg ttttccttac ctgcggaatg
     1141 tatcccagca cctcgccatc atccatgagc gagaggccgg catcctggat accccagtaa
     1201 acaggggcct gctcccctgc cctgaaggtc ttgccgggaa catagtaaat cctcgaatca
     1261 ggtccggtat cggtatggga aatcagcact gaaccgtctg ccgtggcctt cttcccggcg
     1321 gcaatgatcg tacagccgtg agcctccggg accgaaacca tagacgctga cagcattacc
     1381 ataaaaatga ggttgatctt gttcatatga gttttttcta tactgatcag tcaatctgat
     1441 gtacaggcaa aaataagaaa ttattactgg caggaaaaaa acaagaatag aacgctctgt
     1501 caacttttgt tttaacggag gggattccct aaattcgcag caatgaaaaa actgatattt
     1561 ttagtccttg ccatactcat gctggcctca tgcaaccagg gagtctgccc tgcatacagt
     1621 gattcagcca atgacatgaa gaatacactt ctccagaagg gcaacatcag ggccgacggc
     1681 atcaacaggg tttaccgcga cagctaccag aagaagctga gataaaaggc tgcctgcaat
     1741 ctcattccga cagccggaac aagcaaaagg gagtatcagt accagctctg aaccagaacc
     1801 ttcttatcat tctggtattc acaaaccctg taaaagaccg atgaatcatt ctcgatctgg
     1861 tacatccagt acccgtagat acccggggtg acatactgcc tgcgcaggta cagccggtca
     1921 ccttcgttga aaccgcattc cctgctgtag gctgatatct ttccgaaagt gctgtcagca
     1981 gaggtcttga tccagatgag gtgtgggtct ccgaaccgtg caggagccgt atgcctgaaa
     2041 tcctggtagt cacctatata cattcgggtg acatatagcc ggtcctcttc aggctcagtg
     2101 gcagccctgt ttgaggtact gcatgaggcc accagtacaa atatgaacag gagaataact
     2161 tttctgatcg tcatgtcatt ttggttttga tacctaaatt gatactgatg gccggagggc
     2221 ggtgttcgcc gatacttccg gatcagtgtt ataccgaata gaacaggtat ttgtatgtat
     2281 acccgttttc attaagccac cagcgcagtg ccttggcaat ttttcttctc ctcaggagca
     2341 ccttcccggt cacgtctgaa gaaatctggt aatacgtttt catcgcagtt cttatttttt
     2401 gatttagtat ttaattgatt ctctttctgc tgtggaaata ggtcaggccg ttcagttcct
     2461 tcatgtcaat taccacaagt ccgcatctgc ccaatattgc agagaccctg tcaggattaa
     2521 gttcctcacg gactgctgaa cgatgatgga tgattcttga aaggtaaagc ggcatgttgc
     2581 cgggatgctg agcgtcaagc cctgagctcc tccggtaagc aaaacagcct acaaagcttg
     2641 tctccggtgg caacagcctc accagcgagc tgacgaacat ctcaagatgc tttatcaggt
     2701 caagcctgcg caggcttatg agtgttctgg cccggctcag gtcagtcctg ccgtaattgg
     2761 ccccctcatg cgaagacagt attatcagcc ctggtccgcc aaacagtccg gttcttctca
     2821 tgtaacggaa gaaactgaac ccgcagagga taatcatgct tacaatcttg aacaccgccg
     2881 ttgatttcat ttcctggtgg attgattttc ataccaaatg taccagggga aaacgttgaa
     2941 aacaaatcgc aagtgctcat tttacggaca cttggagcca tatcataatg attcgtcaag
     3001 tagttttggc agttcgctta cacaaacggg tgactaacgg cacaaacagg tgttttagtc
     3061 aaaagcggag cgcttttgac caatcacgga tatgcattga ttaaaattta gattcgtccc
     3121 ggctgaaaat tagaatcagc atgccggttt tctaaaataa taccagtgcg tcacgaatga
     3181 ccctcgaggt atcgtctgtg gcgtagagct tgagactcac ctcgggaatg gaatcctgga
     3241 ggatatcggt gatgggattg aaagaatcgc ggacgtcggc agacaacttc atgtcatcat
     3301 actcctcgag cggaatatag atatggacta taagccggta atcagggtta tttatccggg
     3361 tcagggcctt gataatcttc gaaatccaga gcgatgagga ggtattgaag tattcgacat
     3421 tgagtctcag gttcgtgacc ggctgtggtt gaatcacgta attgttcacc cagttaagta
     3481 caggttcgta gatcctggtg gcgttttcag gcagcgacct gcctgtaagt gaaagctcgc
     3541 cgctgctgta tttcagctct atctgcggcg tctttggcgt cttcccgatg attaaatcag
     3601 ttggcttttc cattatttag ttcttcccaa tctaaaacaa ttcgctcaag ttaataatac
     3661 ttctcaaaag taggccatgc tgcggtaagt tcataatcct taatcaaaat ctgatgcatg
     3721 atcatcaact tatagacctg ttcgtcaatg aaacacatga agttcacttt tcctgccggc
     3781 atttcaggcc gttcacagta tgattgtcac attccgggga actccctggc gtagtctgac
     3841 atcgaccgtc tgattacctc ctgtgcctcg gcagtgtcat aaatatctgc aattgtgacc
     3901 cttctcttct ctcccggcag atccttgtag gtaaggaaat agtgcttcag cctctcgatg
     3961 gctatctgcg ggcagtccat gatatcagca tactccccgt acactgcatc gttatacaga
     4021 acggcgatga ttttatcatc ggcctcgttg ccgtcaatca tcctgaatcc gcctatgggc
     4081 ctcgccagca cgagtatgtc accgtgggta atgtccttct ccgtcaggat acagatatcc
     4141 atggcatccc cgtcaccctt tatgccggcc agacccgtct tttccatggt ccggtcagcg
     4201 acatcggcac cggtgagagt cctgggaata aacccgtaca gggccggaac tacattagaa
     4261 tagcgttgcg gtctgtcaag cctcaggtat cctgaaatct tatccatctc atacttgact
     4321 gtgtcggtgg gcaccatctc aacatatgtc atcacgattt cgggggcttt gttcccgata
     4381 tcgatcccgt gccaggggtg cgatctgtac cggcagcagg cggcatcttt ctttttgctc
     4441 attttggttt cagggttagt cagggtatca gttattgaat accaaggtaa gttccgataa
     4501 tttcctggtc ggtatcatca agcagcggca ggcttgggag tctgaacagg tcagcgcctg
     4561 ccattccggt cacgttccgt tcggatgtgc ggataaaact gtatccatgg cgccgggccc
     4621 ctgcaatgat gtcctcatca cctgctccgt cgccgtaagg cagggcgagg gcttcgacaa
     4681 tgccatagat atgcctgtca ataagcacct ttgaaccacc gagctcctcg tcaaggtacg
     4741 cctggaaggc ttcaccaccg ccgagtgctg acttcatatt ataaaggtat tggtgtgaca
     4801 tggtatgcga tccgaaggtg aatggcctga cccctccgtc gaaggtgtaa ctgctcaaac
     4861 gctccacttc gtcccagtcg aggaaggagt ccatcccgat cttcgaggcc cagagaaaga
     4921 aggtcgcctt catcctgtat ctcttcagca gcggggctgc cagagtgtac catgagtggt
     4981 ccccgtcatc aaaagtgatc agagcagcat gcgtggtgag ttcctgctct cctgagataa
     5041 ctgctgccag atcattgaaa tctatcaccc tgatattgtt ttcctgaagg taaaggaggt
     5101 ccttttcgaa ctcttcagcg ctgcgctcat aaaggttgcc ggcgtcaccc tctgtgatcc
     5161 ggtgatacat gagtatcacc acacgcggat tatcgaccgg ctctggatca ggcagcctgg
     5221 tgcatgccgc caccactagg gccacaatga gaaacgccct gacagtcttc tgcatatata
     5281 actggtatga attcaacttt tttgttctcg tggaaatatc attctcatta tcaaacggga
     5341 gaatgactct tttcgtaccc gggccttcag gctatcctct ttcgtttgcc agcttccgca
     5401 ttccctcgat ctcatcgcgc agcctggcag cctcaatgaa gtcgagcgct gcagcagcct
     5461 tctccatagc cttgcgggtt ttgtcaatcg ccttttcaag ctgctcccgt gacatgtact
     5521 ttacaacggg gtcagctgcc acatcaacct taacgggacc ggtataggca tgcagctcca
     5581 cacccttgtg gctcagcccc tttaacatgg tcccgctctt cttcaccacc tgctgcgggg
     5641 ttattcccat ttcagaatta tacttaagct gtcgggcctg ccttcttttc gtctcattga
     5701 tagtctcctg cattgatccg gtaatcctgt cagcatacat caccaccagt ccgttgatat
     5761 tccttgccgc cctgcctgcc gtctgtgtca gcgcccttcc tgacctgaga aaaccctcct
     5821 tgtcagcatc gagtattgcc accagtgaca cttcaggaag gtcaagccct tcgcggagaa
     5881 ggttcacacc cacaagcaca tcgaacacac ccgatctgag cccctccaga atctccactc
     5941 tctcaatggt ctcaacatct gaatggatgt atctcgattt gatgtcgaac ctggcgaggt
     6001 agttattgag ctcctccgcc atcctttttg tcatggtagt aaccagaact ctctcattcc
     6061 tggcagagcg ttttctgatc tcctccatga gatcgtccac ctggttgcgg ctgggccgca
     6121 cttccaccgg cggatcaagg agccctgtag gcctgatcac ctggtcaacg aatacccctt
     6181 cgctcttttc catctcgtat tcagccggtg tggcgctcat gtatatcgtc tgaccagtaa
     6241 gcgcttcgaa ctcctccagc ctcagcggcc tgttgtccaa tgcagacggg aggcggaagc
     6301 catattccac cagggtctgc ttgcgcgagt ggtcacctcc gtacatcgcc cttatctggg
     6361 gaacggtaac atgactttcg tctatgaccg tgatgaaatc cttggggaaa caatcaagga
     6421 ggcagaaagg tcgtgttccg ggcgccctgc cgtcaaaata tcttgaatag ttctcaatac
     6481 cgctgcagta gcccagttcc ttgatcatct caaggtcata ctgcaccctc tgttcaatcc
     6541 ttttggcttc ctccggccga ccggtgtccc gaaagtgcct ggcctgcagc accatgtcat
     6601 cctggatcag cgagacagcc gtgttgatcc ggtccctggt ggtgacaaaa agattcgccg
     6661 gatagatcac atactcatcc aggtcatcac ggtgctgacc ggaaagcgga tcgaaggtgg
     6721 atacctcttc tatctcgtct ccaaaaaaga cgatgcgcag ggcgaggtcg gcataagccg
     6781 ggaaaacatc tacagtatca cccctgaccc tgaatgtgcc gtggctgaat tccagttcat
     6841 tgcgtgcata caggctgtca acaagcctgc gaagcagcaa cgtgcggctg atcttcgccc
     6901 ccctgatcac atgaacggta ttggaacgga aatcatccgg attgccaatg ccgtagatgc
     6961 aggagacaga agagaccaca atgacatcac gcctgccgga gagaagtgat gaggtggcgc
     7021 tgagccggag cttttctatt tcatcattga tggaaagatc cttctctata taggtatcag
     7081 ttaccgggag ataagcctcc ggttgatagt aatcatagta tgacacaaag tattccacag
     7141 cgttttcggg gaagaactcc ctgaattccc cgtacagctg ggctgcgagt gtcttgttgt
     7201 gactcagcac cagcaccggc cggttgagct gagcaatgac attggcaatg gtaaaggtct
     7261 ttcctgatcc cgtcaccccc aacagtgtct gataaggcac tccctgaatc agtcccccgg
     7321 ccagctgccg tatagcctcc ggctggtcgc ctgcgggtgt ataatctgat gtaagcctga
     7381 actccatgat ttgccggtag ggcaaaaata acaaaataga gaggcatggc acaaacatta
     7441 ccatgctatt tattaggtgg cagttcaggc atgctgttaa ctcctttttt ttatttttgt
     7501 attgtcatac atattcagcc gatgtgtcac ccgtgtcgca ctttggtgtt tttattcacc
     7561 agcctccttc ttgccggtcc tggcactgat gccagctgcc agggatatgt actgacgcat
     7621 tacaactcca ggaacggcat cggccatgac aatgtcagga caatcgcaac agacagcagc
     7681 ggctttatct ggcttgcaac gtgggacggc cttacaagat atgacggaac tgactttgtc
     7741 agctatttcc atgatccggc tgacactgcc acaattccat acttttctgt cagccaggtt
     7801 tccattgaca aagaggataa cctctggatt tccactgaca acgggcaact gtctcttttc
     7861 aaccgaacca ccgagaaatt cagccgggta aggagtcttg gaggttaccc tgtcagtgat
     7921 cttacctgct tcaatccggg cccggacgga tttatgtggt ttattctcgg caagggggtg
     7981 ctgaagtaca atcccttatc taaggagact ctgtttttcc cctggagaaa aaatcccggg
     8041 gactcccttc aactggtttt cggcagcaca cgcctgctgt tcgacgaaaa caaagagccc
     8101 tggcttacca ggaacgatgt tctgaaactt aacctggtta cagaccaggc tgccggtgac
     8161 cggtatgctg ccataaattc cttcatcccg atagagagac tgcccggaag gatcggtact
     8221 tttttcgatg aggtcggcta tgcaaagatt gtacatgaca cacccggtaa cctttggctt
     8281 gcatccatga ccggtctgtt ccgttatgac cacgcccgga accgtttcat agaatacagc
     8341 ggtgatttca gaaaaatcag gttcgcgggt gaccagcccg tgatctttta caatcacgat
     8401 accggactta acatctcctt tcctgacagg gacacattgt ttacaatacc gcgtgaaacc
     8461 tgcggcctac ccacaacctg ccttatgact gatcccgggc tgctctggtt ctcacattca
     8521 tggcagggcg gcacatcggc aggagtggtc aaggcagtgt tcactcctca tgaattcaga
     8581 tttataaatc ccttccggga gaagtccggc gaactaaacg tattcggcct tgcaaaagac
     8641 gaacagggtg ccctgtggat tgccgcaagg gacaggaact acaccattcg aatagacagc
     8701 gcaggtgtgc ctgaacgaaa gttcttactg aatgacaggg agataaccga actctggcat
     8761 cccaggtcat tccttcctga taccggctca atgtggatcg gatacttctt caaaagactg
     8821 gttcattatg acctcgcaac cggaaagcca gagtaccact atccgggcaa gagcatccac
     8881 accacctgct atgatgacca gggaaggatc ctgtttgctg acgacggaat aatgaggtat
     8941 gatccggtca cccggacaac ggaacaactg cttgccactg gcgataccat gtacatcttc
     9001 accttccatc gccgcggcga caccctctgg gccggttgca gccacagtta ccttctgaga
     9061 tacaacctga gcaccggaga gcattccccg gtgaaaatcc tggaaggaat gaccaacgtg
     9121 gaagatattt gtgacggcga tgacggaaca ctatggattg ccaccctggg tacaggggta
     9181 tgccactata acccgtacac cggggagaaa ctgttctata caaccgctgc cggcctgtcc
     9241 aacaacacaa cgtactgtat attgagggac cgcaacggga atatctgggt ttcaaccaac
     9301 gacggattgt cggtaatcaa tccggtaacg gggctcataa ggtcattcgg tgaaaatgac
     9361 ggccttatga ttcatgaatt caattcagat gcctcatggg taaatgagga tggcacattc
     9421 atactcggag gcgtcggggg agcggttgaa tttgatcctg ctgaagtgct cagtgaacca
     9481 aagggaatgc caaaacaaca gataatcatc accgagctgg aagtgtcggc actgaaacgc
     9541 gtactcgacg agcctgtata catgactgat acagtggttc ttcgtaaagg tgatgacaac
     9601 tttcacctct catttgtggt acccgaatac cgtaacccgg atatgaccag gttcaggtac
     9661 aggcttggcg gcgatgaggc taactggtac tatactgacc acaatgacag aaacataaat
     9721 ttctcaaatc tctccccggg atggtatgat ctgcatattg aagctactga tatcaacggt
     9781 tcatggagca acagcagact gctgactatc cgggtaaaac cctacttcta ccagaccatt
     9841 ctcttcaaga taacactgcc gctgtttcta ctgacgcttt tctcctttat tgcctggtct
     9901 gttctcaaac agatgaatca gagggaacgt cagaaaaggg acatgctgcg ccagcaggcc
     9961 ctccgtgcac agatgaatcc ccattttatc ttcaacgcac ttaactctat caattacttc
    10021 atctcaaata atgaccggtt gtcagccaac aggtacattt ccgacttttc caaacatatc
    10081 cggacagtcc tcaacaacat gaacgaagac ttcgtcaggc ttagtgtgga gattgatgcc
    10141 ctggaagact acctgaagat tgaacatctg agattcggag acaaatttga ctatgcaatc
    10201 aaagttgatc aggatgtcaa ggcagaaagt ttcaggattt ccccgggatt gatccagcct
    10261 tttgttgaga atgccatatg gcatggggtg atgggactga gcggacgcaa ggggatgata
    10321 accgttgcag tgaagatgaa cggatccgga ttgctgtgca tggttgagga tgacggtgtt
    10381 ggcagggcac gctccgaagc catgaaagac ggaacgctcc ctaagaaatc aaggggaatt
    10441 tcacttgcca cagagaggat gaagatcatt aacaaccttc tcctgaccaa ttaccggatc
    10501 accatttccg atctgtaccc tgaccgtacg gaaaccggta ccagggttga gattgagctt
    10561 ccccttgcac cataatcctg aatcagcgat atgtaactga ccaatcccaa ttctgactcc
    10621 actgatatga tacgggctgt aattattgac gacgaaaaac tggcaagaga cgtaatatcc
    10681 aactaccttc aggattattg tcccgaagtg gaggttgtgg caatggcatc ctccgtgaag
    10741 actgccttga ctgccattca gaagacaaca ccggacctcg tcttcctgga cattgagatg
    10801 cctgacggaa caggttttga tctcctgaat atgttcgaga agattgaatt caagattatt
    10861 tttgtcacag cctactcgga gtatgcaatt aaagcattca ggttcagcgc gctggactat
    10921 cttctgaaac cggtaaaaat tgatgagctg attgacgccg tggccagggt caggtcagct
    10981 gccaccccgg gtataagttc cgagatcatc agctcactgc ttgaaaatct ccggagcaac
    11041 tcaccaagac agtcaaccct gatcattcca aatataaaag gctttgaggt gctcagggtt
    11101 gcagatatca tcatgtgcca ggctgacggc tactgtacca attttcttct ttcaggaaac
    11161 aggaaggttg taagctcacg gaatctgaaa cattttgatg gtttgcttga ggatcagagt
    11221 ttcctcaggg tacaccactc atatctgatt aacttagaac atgttaccgg gtacacccgt
    11281 cagggtgaaa taattctgtc ggagggactg aaggcattcc tgggagattc atacaagaac
    11341 gaattcgtca gaaggttcac cggaaaatag gattctgttg gatctcccgg gcgttctatt
    11401 gccccaacct caggttctgt aagttgaagc cattcggtga taacacctga tcatcatttg
    11461 agttattttt accgacagat atggcagttt ctgtctctca ataacgccat atctgtatga
    11521 agcagttctt ccccccagat ccgcaagcaa tgcggacaca aaatgagccc gattcgccgg
    11581 gctcatttct tttacaatac aatatccgaa aaccttcagg ccaggcagta taaatgaaaa
    11641 aagagatgct gatacatctc tttttcctgt ttacctatcc ctgagaatac taacccaggt
    11701 atcccttcag gagtttgctg cgggatgtgt gtcgcagcct gcgaattgcc ttctccttta
    11761 tctgtctcac cctttcacgg gttaggccga acttctcccc tatctcttca agagtcatct
    11821 cctggcaggc aatgccaaag aaataacgta ttatatccct ctcccgctcg gtgagcgttg
    11881 ccagtgcacg gtcaatctcc tttgagagag actcgtctat cagcatcttg tccgcaatcg
    11941 gcgaatcaac gttggtgagc acatccagaa ggctgttgtc ctcaccttcc acaaacgggg
    12001 catcaactga tacatgacga cctgaaacct tgagagtgtc agtgactttc tccttcggca
    12061 ggtcaagcac ctcggcaagc tcttcagggg aaggaatacg ctcattctcc tgctcaaacc
    12121 ttgcaaatgc cttattgatc ttattcagcg aaccaacctg atttagcgga agacgaacta
    12181 tccttgactg ctctgcgaga gcctgaagga ttgattgcct gatccaccag acggcatatg
    12241 atataaattt gaaacccctg gtttcatcaa atttttctgc agccttgata aggccgaggt
    12301 taccctcatt aatgaggtct ggaaggctaa gcccttgatt ctgatactgt ttggccactg
    12361 agacaacaaa gcggagattg gctttggtaa gcttctccag cgctgccctg tcacccttct
    12421 tgatccgttg tgctaactct acctcttcct caaccgatat caactcttct ttaccaatct
    12481 cctgcagata cttgtccaac gacgcacttt cacggttggt aatggatttg gtaattttaa
    12541 gttgtctcat tctctatacc ccttcgaaaa aattttcgca aagttactgc taattaagtt
    12601 actaaacaaa tatatattca accactccgc atgcggttcg tctctcccgg caaaccgctg
    12661 atcttctgat ttttataact tactctcatt cttacctcct ggtctggtac ttgaagtatg
    12721 ttccatccgg tgtaaagcct tcgactgagg tcagactctt ttcgtcaccg gttgccttgc
    12781 ggacatccga accgctgttc acttttactc cgttcacgtc aattatgacc gttcccttcg
    12841 aaaggccaag atcgcggaac cggccgtccg aaattgaggt gatctttaca ccgcctgata
    12901 ttttaaactg acttttctcg cctgcagtga ccggcgtgaa tgtagcgccg aacactgatg
    12961 aggactgtgc cccgggagct acaacgcccg ttccgccctc aatattgcgg agaaccacat
    13021 tctttttatc ctgtctcccc ttcctcaggt aggtcacctc cagcctgtca cccggcctgt
    13081 aacggctcac tttctcctga agatccgccg tggtttcaac cggttcaccg ttgattgcga
    13141 tgatcacatc cttttctgcc agacc
//
LOCUS       DAOPXU010000076        13091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_5252_length_13091_cov_10.491102, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000076 DAOPXU010000000
VERSION     DAOPXU010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13091
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5252_length_13091_cov_10.491102"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1538
                     /locus_tag="PKK03_08505"
     CDS             120..1538
                     /locus_tag="PKK03_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84482.1"
                     /translation="MRYAIPFLLIAAACISCDPLANPVSGRLSFSSDTISFDTVFSGY
                     GSATLELRVKNLEDDPLLIDRIWLGGGSGSPFRINIDGQPATEAQNVTLARDDSLFIF
                     VIVTIDPTGADLPLAVTDSVNFISGSHSGRVILEAWGQDVRLVDEDILTPAVWTEGKP
                     YVIRDHLLVDTLATLTLEPGTRVYMHHGTMVTVAGSLVAAGTAEKRITIATDRLEKEY
                     RDVPGRWKGLRFQSCSHGNILNHTDIRNAEIAVRIDGLPSSVPDLSMNSTTLMHNTVA
                     SLAAKHADLFAVNSLFAHSGFSTVSLAEGGSYEFIHCTISNSWEYSFRSEPALLIRQG
                     TGVMATVSVINSVVSGSLGDELMIQAPVSAVAAAFRADSSLIKVDTLAADWYSTALFT
                     RVITTRPPLFIDENAWDFRPDTLSPLLDRAGKTEMKLWPADIRNKPRPAFDGPDIGAF
                     ERQGGEKRKEEESGMAGMRVRH"
     gene            1626..2594
                     /locus_tag="PKK03_08510"
     CDS             1626..2594
                     /locus_tag="PKK03_08510"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNX84483.1"
                     /translation="MALLATMLSQASGQDFTVMFYNVENLFDTVDDTAKNDEEFLPGG
                     SRRWTASRYHRKLNAVARAVAVAGEWELPSLVGLCEVENGEVLRDLAYGTLLSAGNYG
                     IAHRESPDPRGIDLALLYRRDCFSVAHTDAWLPADADSMPVLTRNILYVKLTAGADTL
                     HIVICHLPSRRGGVLAAGDLRERMVSLAGAGIDSIMSASGGRAAVIVMGDFNTSPREP
                     FLEGFAREAGLVNLSAEMAGQGRGSYRYHGTWEMIDQLLVSVPMTDSTGVFYALPGSF
                     RVVDAPFLLTADETYPGKKPYPTYGGYRYSGGYSDHLPVTVRLSVR"
     gene            complement(2598..4010)
                     /locus_tag="PKK03_08515"
     CDS             complement(2598..4010)
                     /locus_tag="PKK03_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNX84484.1"
                     /translation="MKIFPDDAIFEIVARVADRENVEAYVIGGFVRDHIMGRVNPDRD
                     IDIVVLGDGPAMARAVAREISRNIKVTVFKTFGTAMFRYRNSDIEFVGARKESYSADS
                     RKPSVTPGTLEDDQNRRDFTINALAISLNRDNPGEVLDPFGGIDDIRNGIIRTPLDPD
                     TTFSDDPLRMMRAVRFAAQLGFRIDGETLASIARNTERIKIVSGERITTELNKIMAAA
                     RPSAGFLLLDQTGLLELILPEIARLKGVEDKEGKGHKDNFHHTIKVLDNLAEKSEDLW
                     LRWAALLHDVAKPATKKFVQGTGWTFHGHEFIGAKMVPEIFKRLRLPLNDRMKYVQKL
                     VGLHLRPISLVQEEVTDSAVRRLLFEAGEDIDDLMLLCEADITSGNRMKVLKHRQNFA
                     LVRRKLKEIEEKDAVRTFQPPVHGEEIIATFKIPPGPLVGVIKAAIKEAILDGIIPND
                     HDAAREFMIRKGREMGLEPA"
     gene            4162..4524
                     /gene="trxA"
                     /locus_tag="PKK03_08520"
     CDS             4162..4524
                     /gene="trxA"
                     /locus_tag="PKK03_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNX84485.1"
                     /translation="MAEHLTKDTFLEKVFNYEQNKEWKYSGDKPCIIDFYADWCGPCK
                     MVAPILEELSKEYDGKLYVYKIDTEAEQELASVFGIRSIPSLLFVPMEGQPQMAMGAL
                     PKEVFEKAFKDIFGIEKN"
     gene            complement(4734..4931)
                     /gene="xseB"
                     /locus_tag="PKK03_08525"
     CDS             complement(4734..4931)
                     /gene="xseB"
                     /locus_tag="PKK03_08525"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNX84486.1"
                     /translation="MAKKEIGFNEAMNGIEQILKNIEEGEPDIDRLSEDVRKAAELIK
                     ICRRRLRETGEKIDGIMEELR"
     gene            complement(4934..6190)
                     /gene="xseA"
                     /locus_tag="PKK03_08530"
     CDS             complement(4934..6190)
                     /gene="xseA"
                     /locus_tag="PKK03_08530"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNX84487.1"
                     /translation="MNRDAKISLTELLGLVRDKIYEALPEAFWVVAEIAEMKVNTAGH
                     CYLELTGSDTPGGRITSRVRATIWVSKYRSLNTLFNASTGIPLRAGITILFRATVEYH
                     ELYGLSLNITDIDPAYTAGDMALRREAVIRRLTAEGVLSMNSGLPLALYPRNIAVVSS
                     SKAAGYQDFADHLLNNPYRYVFKATLFEAVMQGEETEASVIGALDRISEKTGKFDAVA
                     IIRGGGSTTDLSWFDNYAIAFHVTQFPLPVLTGIGHEKDQSVTDMVAWKALKTPTAVA
                     GFLVDRTLECENRIISMAGDMASAVTGALDAAGEQLSSFQNRAAATARLVVRVNHDRL
                     GYHTETLRRSARNVLQKAVDKTGRLEQSLRHLDPAGVLKRGFTLTSRNSIIIRSAAEL
                     EEGDNITTHFEKGTVKSTVKQINNKK"
     gene            complement(6187..7836)
                     /locus_tag="PKK03_08535"
     CDS             complement(6187..7836)
                     /locus_tag="PKK03_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNX84488.1"
                     /translation="MNRASAALAPLLFVIAGIIPAKAQQVYPGYDDLWFYRVWFSDKT
                     NSISEYSPEELLSPAAITRREKYGIPLVTESDIPVSSQHIQTLAGEGFRLRCTSKWLN
                     TALFTTTGSKDTDMSGEYPFIDSIQLVKHPVDPVKTNYSKYGKTELRTTTEIFDPRLP
                     LNGNVLHESGFTGRNITIAVLDAGFVNADKIEALEPLRKRGGIIATRDFITGSSFVYD
                     YHTHGTSVLSILAGSLPGIISGTAPGADYMLLRTEDDFSEFPVEEDYWIAAAEYADSA
                     GADIITSSLGYFEFDDPSMDYSFSDMDGDRPFITRGADMAFSKGILVVNSAGNERNNV
                     WLRIIAPSDGDSVLCVGAVNYDLTISDFSSAGYSSDGQVKPDVVAPGVLIPIQFEPGE
                     WHSGSGTSFSCPVISGLSASLMQAVPDASPLELINALHESSDRYTRPDSLYGYGLPDF
                     LKTLRLLEEEHTFRPEVIMSAGPNPFTDEILFWFHEPPGSLTVTITGTNGTTMLEKHF
                     PTFAARSFRLDGLGSLAEGIYLVRTVTDQGKKVFKMIHTDR"
     gene            complement(7833..8609)
                     /locus_tag="PKK03_08540"
     CDS             complement(7833..8609)
                     /locus_tag="PKK03_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84489.1"
                     /translation="MENKLIIILSLLLLSLTVLPQNVTDAAGKKQGPWVKKYPNGKIM
                     YEGTFVNDKPVGLLKRYTEEGVLLSELTYTPGRDEASAVFYYPGGLKAAEGKYVARAR
                     EGLWKFWSATQPHYLICEEYYHNDTRHGLSQKFYPDSTLAEKVAWDMGNRTGEWLQYY
                     PDGAICLRAEFVAGRLEGPFSYYHPNGNLQYEGRYKEDHRTGDWMVFNEDGALKQIIT
                     YREGVPSDPKLADQETKFLDDLEKNKGKIEVTDITGSVIK"
     gene            complement(8734..10659)
                     /gene="dnaK"
                     /locus_tag="PKK03_08545"
     CDS             complement(8734..10659)
                     /gene="dnaK"
                     /locus_tag="PKK03_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNX84490.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGNEPVVIPNSEGKRTTPSVVAFLE
                     NGERKVGDPAKRQAITNPRKTVSSIKRFMGRSFDEVTNEMKKMTYEVIRGDNNTPRVK
                     IDDRNYSPQEISAIVLQKMKKTAEDYLGQEVTEAVITVPAYFNDSQRQATKEAGEIAG
                     LKVKRIINEPTAASLAYGLDKKAHDLKIAVFDLGGGTFDISILELGDGVFEVKSTNGD
                     THLGGDDFDHLIIDWLADEFQREEGIDLRKDPMALQRLKEASEKAKIELSSTSTTEIN
                     LPYIMPVNGIPKHLVKSLTRAQFEKLTDKLINAVIGPCQKALDDAGMKTSDIDEVLLV
                     GGSTRIPAIQQIVEKFFGKTPSKGVNPDEVVAVGAAIQGGVLTGEVKDVLLLDVTPLS
                     LGIETMGGVMTRLIESNTTIPTRKTETFTTASDSQPSVEIHVLQGERPMAAGNKTIGR
                     FHLDGIPPAPRGIPQIEVTFDIDANGILHVSAKDKGTGREQRIRIEASSGLKEDEIRR
                     MRDEARANEAADKAAREMADKVNAADSMIFQTEKQIKEYGDKIPSDKKGRIENALGQL
                     REAHKNRDITAIDRALAEINAAWQAASEDMYKQAQGGPGPQAGPDAGPQSGPTGGTTG
                     GKGGDEQVTDVDFEEVK"
     gene            10896..11711
                     /gene="panB"
                     /locus_tag="PKK03_08550"
     CDS             10896..11711
                     /gene="panB"
                     /locus_tag="PKK03_08550"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNX84491.1"
                     /translation="MSVQKTVKIVTTHVLYEMKLKGEKISMLTAYDYSMARILDEAGI
                     DVILVGDSASNVMAGFDTTLPITLDNMIYHAASVVRGVKRALVVVDLPFGTYQGNSRE
                     ALASAIRIMKETGASAVKLEGGREIVESIERIMSAGIPVMGHLGLTPQSIHKFGTYVV
                     RARDEEEARKLKEDAKIIEKAGCFAIVLEKIPAALAGEVAESLKVPVIGIGAGPQVDG
                     QVLVLHDMLGINMEFSPRFLRRYHNLYDEIKGAVHHYIADVRSGNFPNEKEQY"
     gene            11777..12463
                     /locus_tag="PKK03_08555"
     CDS             11777..12463
                     /locus_tag="PKK03_08555"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNX84492.1"
                     /translation="MREGFPEVLYEDNHIIIVNKRSSDLVQGDGTGDESLDNIVRGYI
                     KEKYGKPGDVFLGVVHRLDRPVSGCVVYARTSKALTRLSELFRTRDVKKSYWAIVTDR
                     PPAEEGSLQNYLKKNEKQNKSYVYEKEVKGSKSAELSYKILSRSERYYLLEIDLLTGR
                     HHQIRAQLAAAGCPVRGDLKYGARRSNEGGGISLHSRRVSFIHPVRNEEINVVAPLPD
                     DRIWKLFKNV"
     gene            complement(12628..>13091)
                     /locus_tag="PKK03_08560"
     CDS             complement(12628..>13091)
                     /locus_tag="PKK03_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84493.1"
                     /translation="TKALSTVIGIVAAIFAGTTTLSAQETKNVEKKVIVVTVEEDSIV
                     KDSAVIEGDTIRYEGGNLIIETGEGKRIIRKPGKGSRMVWTATTADFPERGEAAGAAP
                     GRAMIRMQEEKEGVFYNISLDGVVVSIRAPKEKADEADKILSEVKKILMKK"
BASE COUNT         2918 a         3296 c         3468 g         3409 t
ORIGIN      
        1 tatgcatttg actttactct ttcagagatc cgcagctata cttacgggac acatgagctg
       61 accctgcttg cccgctttgg agacaacccg aggcggtaca ggtggatcaa caaatactga
      121 tgaggtacgc cataccattc ttacttattg ccgcagcctg cataagctgt gacccgcttg
      181 ccaatcctgt cagcggccgt ctgtccttct catcagatac catttcattt gacaccgtct
      241 tttccggata tggctcggcc acccttgaac tacgggtcaa aaaccttgaa gatgaccctc
      301 tccttattga ccgcatctgg cttggcggag gctccggttc accattcagg ataaacattg
      361 acggccagcc tgcaaccgag gctcaaaacg taacactcgc cagggatgac agcctcttca
      421 tctttgtcat tgtgacaatt gatccgacag gagctgacct gcctctggcc gttactgatt
      481 ctgtgaactt tatttcagga agccattccg ggagagttat ccttgaggcc tggggtcagg
      541 atgtacggct tgttgacgag gatattctta ccccggcggt atggaccgag ggtaagcctt
      601 atgtgatcag ggaccacttg ctggttgaca ccctcgcgac cctgacactg gaaccgggaa
      661 caagagtcta catgcaccat gggaccatgg tgacggtggc cggatcactc gtggcggccg
      721 gtacggctga aaaaagaata acaattgcaa ctgaccgcct ggaaaaggag tacagggatg
      781 tgccaggacg gtggaaaggg ctcaggttcc aaagctgcag tcatggtaat atactgaatc
      841 atactgacat acgcaatgct gagattgcag taagaattga tggtctgcca tcgtcagtcc
      901 ctgatctcag catgaacagc acaaccctga tgcacaacac tgttgcctca cttgcagcca
      961 agcatgcgga tctgtttgcg gtcaactccc tttttgccca ttccggtttc agtactgtct
     1021 cactggctga aggcggcagt tacgagttca tccattgcac aatcagcaac agttgggaat
     1081 acagtttccg ctctgagccg gcgcttctta tcagacaggg caccggggtg atggcgaccg
     1141 tctcggtgat aaattcagtt gtcagtggga gcctgggtga cgagctcatg atacaagctc
     1201 cggtatcggc tgttgctgcg gccttcaggg ctgacagctc actaataaag gttgacaccc
     1261 tggctgctga ctggtattca acggctttgt tcacgagggt gatcaccacc cggcctcccc
     1321 ttttcattga tgagaatgcc tgggatttca ggcctgatac gctctcgccc ctccttgacc
     1381 gtgccgggaa gactgagatg aagttatggc cggccgatat cagaaacaag ccgcggccag
     1441 ccttcgatgg tcccgatata ggggcgtttg agaggcaggg aggagaaaag cggaaagagg
     1501 aagaatccgg catggccggc atgcgggtca ggcattgaca aatctgacta ttagctgtat
     1561 atttgtcctg ctgctatttt aatctgtgct ttattcatcg tgtgatgagg gtgacactct
     1621 caattatggc attacttgca acgatgctgt cgcaggcatc cgggcaggat ttcaccgtga
     1681 tgttttataa cgtcgaaaac ctgtttgata ctgtcgatga taccgcaaag aatgatgagg
     1741 agtttctccc cgggggatca aggaggtgga cagcatcgag gtaccacagg aagctcaatg
     1801 ctgttgcgag agctgtggca gttgccgggg agtgggaact cccttccctg gttggtctct
     1861 gcgaggtgga aaacggagag gtgctgagag atcttgctta cggtactctc ctctcagccg
     1921 gcaattacgg aattgcccac agggagtcac ccgaccccag gggaattgac ctggcgcttc
     1981 tttaccggcg tgactgtttt tcagttgcac atactgatgc atggctaccg gcagatgcag
     2041 acagcatgcc agtactgacg cggaatattc tctatgtgaa gctcaccgca ggagctgaca
     2101 cgcttcatat tgttatctgt cacctgccgt cgcggagagg aggcgtgctg gcggcaggtg
     2161 atctgaggga aagaatggtc agtctggcag gcgccgggat tgactccatc atgagcgcct
     2221 caggagggag ggcagcggta atagtcatgg gcgacttcaa cacctctccc cgagagccgt
     2281 tcctggaagg atttgcccgg gaggccggtc tggtcaacct ctcggctgag atggcagggc
     2341 agggcagggg ctcctacagg taccatggaa cctgggagat gattgaccag cttcttgttt
     2401 cagtgccgat gacagacagc acaggggtct tttatgcatt gcccggcagt ttccgtgtag
     2461 tggatgcccc ctttctcctg acagctgacg agacttaccc gggcaaaaag ccctacccga
     2521 cctatggggg ttacaggtat tcaggagggt attccgatca ccttccggtg actgtcaggt
     2581 tatccgtaag gtgaccttca tgccggctcc agtcccatct cccgtccttt acggatcatg
     2641 aattcacggg cggcgtcgtg atcattgggt atgatgccgt cgagtatggc ctccttgatg
     2701 gcagctttaa tgactcccac cagcgggccc ggaggtattt tgaatgtggc aattatctct
     2761 tcgccatgga ccggaggctg gaatgtccgc acggcatctt tctcctctat ttcctttagt
     2821 ttgcggcgca ccagcgcgaa gttctgacga tgcttcagca ccttcatcct gttgccggag
     2881 gtgatgtcgg cctcgcatag caacatgaga tcgtcgatgt cctcgcctgc ttcgaagagg
     2941 agccttctta cggccgagtc agttacttct tcctggacga gtgagatagg tctcaggtgc
     3001 aggcccacga gcttctgcac atatttcatc ctgtcattga gcggaagtct gaggcgtttg
     3061 aagatttccg ggaccatctt ggccccgatg aattcatgtc cgtgaaacgt ccaccccgtg
     3121 ccctgcacga acttcttggt ggcaggcttt gccacatcgt gaagaagggc tgcccagcgc
     3181 agccacaggt cctcactttt ctccgccagg ttgtcaagaa cttttattgt atggtggaag
     3241 ttgtccttat gcccttttcc ttccttgtcc tcaacaccct tgagccttgc aatctccggc
     3301 aggatcagtt cgagcagacc ggtttgatcc agcagcagaa agccggctga cggcctggcc
     3361 gcagccataa ttttgttaag ctccgttgtt atcctttctc ctgagacgat tttgatcctt
     3421 tccgtattgc gggcgatgga ggccagtgtc tctccatcaa tccgaaaacc taactgggcc
     3481 gcgaacctga cagccctcat cattctgagc ggatcatcgc tgaaagtggt gtcagggtca
     3541 agcggagtgc ggatgatgcc gttgcgtatg tcgtcaatcc ccccgaacgg atctagtact
     3601 tcgcccggat tatccctgtt caggctgatg gcaagggcat tgattgtaaa gtcgcgcctg
     3661 ttctggtcgt cttcaagagt ccccggcgtg acagacggct tgcgggagtc ggctgagtat
     3721 gactctttcc tggcgccaac gaactcgata tcagagttcc ggtacctgaa catagccgta
     3781 ccgaaggtct tgaatacagt caccttaatg ttgcggctga tctcacgggc aacagcgcgg
     3841 gccatagctg gcccgtcgcc aagaaccaca atgtcaatat ccctgtcggg gttaaccctg
     3901 cccattatat gatcacgtac aaaaccgccg atgacatacg cttccacgtt ttctctgtca
     3961 gcaacgcggg cgactatctc aaagatggcg tcatcaggga aaatcttcat aatacgtatg
     4021 acattatagc cgaatccggt gacaaaatta caatttatac cctaacttga agaaaaattg
     4081 atctcctgag cctcccggca tgccaattgc aaggactgaa cattacccgt aaaaatctgt
     4141 taaacaatta aaacaataaa tatggcagaa catttgacaa aagatacttt tctcgagaaa
     4201 gtattcaatt atgaacagaa caaggagtgg aaatacagtg gcgacaaacc atgtattatt
     4261 gacttttatg ccgactggtg cgggccatgt aagatggttg cgcccatcct tgaggagctt
     4321 tcaaaggagt atgatggcaa gctttatgtg tataagattg atacagaggc agaacaggag
     4381 cttgcatcag tattcggaat aaggagtatt ccctcacttt tgttcgttcc gatggagggt
     4441 cagcctcaga tggcaatggg tgccctgccc aaggaagtgt ttgaaaaagc cttcaaggat
     4501 atatttggaa tagaaaaaaa ctagtttgtt aataaatatt ttactttttt cacagatttg
     4561 gaaaacaatt tcgtaactta atcgttctat ttactgaagg tagttttcta acagaagagt
     4621 tagttttctg gaatttaggt tagggtttcg agttgaagcc ccgctgcggc ggggcttcct
     4681 tgattttatg cccccgggaa tggcagggca gagcggagtg ccggccgcag gaattacctg
     4741 agttcttcca ttatcccgtc aattttttca ccggtttcgc gcaggcggcg ccggcatatc
     4801 tttatgagtt cagcggcctt cctgacatcc tctgacaatc tgtcgatatc aggttcacct
     4861 tcttcgatat ttttaagtat ctgttcaata ccattcatgg cttcattgaa cccaatttct
     4921 ttttttgcca ttgttatttc ttgttgttta tctgtttaac agtgctcttt accgtaccct
     4981 tttcaaaatg ggtggtaatg ttgtcaccct cctcaagctc agcggctgaa cgtattatga
     5041 tgctgttcct ggaggtcagc gtaaaccctc tctttagaac tcctgccggg tcaaggtggc
     5101 gcagtgactg ctcgagtctg ccggttttgt ctacggcctt ttgaagtaca ttcctggccg
     5161 agcggcggag tgtctcggtg tggtatccca gtctgtcatg gttgaccctg acaaccagcc
     5221 ttgcggtggc ggcagcacgg ttctgaaatg atgagagttg ttcaccggct gcatcgagag
     5281 cccctgttac cgctgatgcc atatcgcctg ccatgctgat tatcctgttt tcacattcaa
     5341 gtgttctgtc gaccaggaat cctgctacgg cagtgggagt cttcagggct ttccatgcaa
     5401 ccatgtcagt gactgactgg tccttttcat gcccgatgcc tgtcagtaca ggcaggggga
     5461 actgggtcac atgaaatgct atcgcatagt tgtcaaacca gctcaggtca gtggtggatc
     5521 cgcctcccct tattattgcc accgcgtcaa atttccctgt tttctctgaa attctgtcaa
     5581 gggctccgat gacagatgcc tcagtttcct caccctgcat tactgcctcg aaaagagtgg
     5641 ctttaaaaac atatctgtaa ggattgttca gcaggtgatc ggcgaagtcc tggtacccgg
     5701 cagcctttga tgaagagaca acggcaatat tccgcgggta taaggcaagg ggaagccctg
     5761 agttcatgga gagcacgcct tcagcggtaa gccttcttat taccgcctca cggcgaagcg
     5821 ccatatcacc tgcggtgtat gcaggatcaa tgtcagtaat gttcaggctg agtccgtata
     5881 gctcatggta ttccactgtc gccctgaaga ggatggttat cccggcgcgg aggggtatgc
     5941 cggtgctggc attgaagagc gtgttgagtg acctgtattt tgatacccag atggtagctc
     6001 tgaccctgga ggtgattctt ccgccgggtg tgtcgctgcc ggtgagttca aggtagcagt
     6061 gccctgcagt attgaccttc atctcggcta tctcggcaac aacccagaat gcttccggca
     6121 gggcttcgta tatcttgtcg cggaccagcc ccagcagttc tgtcagtgat atttttgcat
     6181 cgcggttcac ctgtctgtgt ggatcatttt aaagaccttt tttccctggt ctgttactgt
     6241 tctgaccagg taaattccct ctgcaaggct gccgagtccg tcaagcctga atgagcgtgc
     6301 ggcaaaggtc gggaagtgct tttccagcat cgtggtgcca tttgttcccg ttatggtcac
     6361 agtcagtgaa cccgggggct catggaacca gaagagtatc tcgtcagtga aaggattggg
     6421 gccggcgctc ataatcactt cgggtctgaa ggtgtgttcc tcctcgagca gcctgagtgt
     6481 tttcaggaag tcaggcaatc cgtatccgta gagtgaatcg ggcctggtgt acctgtcgct
     6541 gctctcgtgg agcgcattga taagttcaag aggtgaggca tccggaacag cctgcatcag
     6601 tgaggctgaa agaccgctga tgacagggca tgagaaagat gtaccgctgc cactgtgcca
     6661 ctctccgggc tcaaattgaa tgggtatcag aacacccggt gccaccacgt caggtttgac
     6721 ctggccatct gatgagtaac cggccgatga aaagtcggat atggtgaggt cgtagttcac
     6781 cgcgcccacg cacagtacgc tatcgccgtc agaaggggca attatccgga gccatacatt
     6841 atttcgttca tttccggcac tgttgacaac gagtatcccc tttgagaagg ccatgtcagc
     6901 acccctggta ataaaaggtc tgtctccgtc catatcgctg aacgaataat ccatcgacgg
     6961 atcgtcaaat tcaaagtaac cgagggacga ggtgatgata tcagctcctg cgctgtcagc
     7021 atattccgcg gcggcaatcc agtaatcttc ctccacggga aactcggaga aatcatcttc
     7081 ggtgcggaga agcatatagt ctgctcccgg ggcagtgccg ctgatgattc ccggcagtga
     7141 tcctgcaagg attgaaagca ctgatgtacc atgggtgtgg taatcataaa caaagctgct
     7201 gccggttatg aaatcacggg tggcgattat accgccccgc ttccttagcg gttcaagggc
     7261 ttcaatttta tcagcattga cgaatccggc atcaagaacc gcaatggtta tgttgcgtcc
     7321 tgtaaatcct gattcatgaa gcacatttcc gttgagagga agacgcgggt caaagatttc
     7381 agtggtcgtg cgcagttcgg ttttgccgta tttgctgtag ttcgttttga caggatcaac
     7441 cggatgcttt accagctgaa tgctgtcgat aaagggatac tccccggaca tgtcagtgtc
     7501 tttggacccg gtagtggtaa agagagcggt attaagccat tttgacgtgc accttaacct
     7561 gaagccttcc ccggcaaggg tctggatatg ctgactgctg acggggatgt cgctttctgt
     7621 aaccaggggg atgccgtact tttccctcct ggtgattgcg gcaggtgaaa gcagttcctc
     7681 aggtgagtat tcactgatgc tgtttgtctt gtcgctgaac cagacccggt aaaaccacag
     7741 gtcgtcatag ccgggatata cctgctgagc tttcgccggt atgatgcctg cgataacaaa
     7801 aagcaacggt gcaagggcag ctgaggctct gttcatttta ttactgatcc cgtgatgtct
     7861 gtcacctcta tttttccctt gttcttttca agatcatcga ggaatttcgt ttcctggtct
     7921 gccagtttcg ggtcagatgg gacgccctcc ctgtaggtga tgatctgttt cagggctcca
     7981 tcttcattga aaaccatcca gtcgcctgtc ctgtgatcct ctttatagcg cccttcgtac
     8041 tgcagattgc cgttggggtg gtagtaggag aaggggcctt caagcctgcc tgccacgaat
     8101 tcagctctga ggcatattgc cccgtcaggg tagtactgca gccattctcc ggtgcggttg
     8161 cccatatccc atgccacctt ctctgccagg gtgctgtcag ggtagaactt ttgtgacagt
     8221 ccgtgcctgg tatcgttgtg ataatactcc tcgcagataa gatagtgcgg ttgggtggcc
     8281 gaccagaatt tccagagtcc ttccctggca cgtgcgacgt atttgccctc cgcagcctta
     8341 agcccgccgg ggtagtaaaa cactgcagat gcttcatctc tgcctggggt gtatgtcagt
     8401 tccgaaagaa gcaccccttc ttcggtatat cttttaagca gtccgaccgg tttgtcattc
     8461 acgaaggtac cctcgtacat gattttgccg ttggggtatt ttttaaccca tgggccctgt
     8521 tttttccccg cggcatcagt cacattctga ggcaggacgg ttaaggagag cagcagcagt
     8581 gaaagtatga taattaattt gttttccatg gtttacttat ctgttacaaa gatataacca
     8641 aatagttcgc cctgttcgta tccggggcac cagaaaaagg aaagccgcag caacttttgc
     8701 tgccgcggct ttcactgatg gtttatatag tgattacttg acttcctcga agtcgacgtc
     8761 agttacctgc tcgtctccgc ccttgccgcc ggttgttcct ccggtggggc ctgactgcgg
     8821 gcctgcgtca ggacctgcct gcgggccggg acctccctgg gcctgtttgt acatatcctc
     8881 ggaggcagcc tgccatgcgg cgttgatctc cgcaagtgca cggtcgatgg ccgtgatatc
     8941 cctgttttta tgtgcttcgc gaagctggcc gagggcattc tcgatgcgtc ccttcttgtc
     9001 tgaaggaatc ttgtcaccgt actccttgat ctgtttctca gtctggaaga tcatgctgtc
     9061 agcagcgttg accttgtcgg ccatttctct tgctgcctta tcagccgctt cgttggccct
     9121 ggcctcgtca cgcatacggc ggatctcatc ctccttaagg ccagatgaag cctctatccg
     9181 tatcctctgt tcccttccgg tgcctttatc cttggccgag acatgaagaa tgccgttggc
     9241 gtctatgtca aaggtgacct cgatctgcgg gattccgcgc ggggcaggcg ggatgccgtc
     9301 gagatggaag cggccgatgg tcttgttgcc ggcggccatc gggcgttcac cctgcagcac
     9361 gtgtatctca accgaaggct ggctgtcgct tgccgtcgtg aatgtctcgg tcttgcgtgt
     9421 ggggatggtg gtgtttgatt cgatgagtct ggtcatcaca ccgcccatgg tctcaatgcc
     9481 aagtgacagc ggggtgacgt cgagaagaag cacatccttc acttccccgg tgaggacacc
     9541 gccctgaatg gcagcgccca ctgcaaccac ttcgtcggga ttgacaccct ttgaaggagt
     9601 cttgccgaag aacttctcaa ctatctgctg gatggcaggt atgcgggttg aaccgccgac
     9661 gagcagcacc tcatcgatat ccgatgtctt catgccggcg tcatcaagcg ccttctggca
     9721 aggaccgatg actgcattga tgagcttgtc ggtaagcttt tcaaactgtg cccttgtaag
     9781 tgactttacc aggtgtttgg gaataccgtt aacaggcatt atatacggga ggttgatctc
     9841 cgtggtcgag gtgcttgaaa gctctatctt ggctttctcg gaagcttcct tcagtctctg
     9901 gagagccatc gggtctttgc ggaggtcaat tccttcctct ctctggaact catctgcaag
     9961 ccagtcgatt atcaggtggt caaagtcatc accgccgagg tgggtatcac cgttagtcga
    10021 cttgacctcg aagaccccgt cgccgagctc aagaattgag atatcgaatg tgccgccgcc
    10081 aaggtcgaac actgcgatct taagatcatg ggcctttttg tcaaggccgt aagccagtga
    10141 cgctgccgta ggctcgttga tgatacgctt gaccttcagt cctgcgatct caccggcttc
    10201 cttggtagcc tggcgctgtg agtcgttaaa gtatgccgga acggtaatga cagcctcagt
    10261 gacttcctgg ccgaggtaat cctcagcggt tttcttcatt ttctggagca cgatagccga
    10321 gatctcctgc ggagaatagt tgcggtcatc aatcttgacc ctcggggtgt tgttgtcacc
    10381 gcggatgacc tcataggtca tctttttcat ctcgttggta acctcgtcaa aacttcttcc
    10441 catgaagcgt ttgattgatg aaacggtttt acgcggattg gtgattgcct gcctcttggc
    10501 cgggtcaccc accttgcgtt caccgttctc aagaaaagca actaccgaag gggtagtacg
    10561 cttgccttca ctgttgggta ttaccacagg ctcgttgcct tccatcactg aaacgcagga
    10621 gttggtggtt cccaggtcaa taccgataat ttttcccatt gttatactga tttatatgct
    10681 aagtttcttt ttaatactcc tcagaagtga caatgattgt gccattgccc tcaggccgga
    10741 gttattatga aattatgtca tggcaacgcg aatccgccgg cagtcaaata tttcacgtga
    10801 atgacaaaac ggcagaaccg gccaaaagaa tctggtttaa tggcattcaa ccttcttatt
    10861 ttgtagcttt gcaggtgaca ataaaatgcg aagagatgtc agtacagaag accgttaaga
    10921 tagtgaccac ccatgtgctt tatgagatga agctgaaggg tgagaagata tcgatgctta
    10981 cagcatatga ttattcaatg gcaaggatac tcgacgaggc aggcattgat gtgatcctgg
    11041 tgggagactc agcgtcaaat gtgatggcag gctttgacac aacattgcca atcactcttg
    11101 acaacatgat ttatcatgcc gcttcggtgg tcaggggggt taagcgagcc ctggttgtgg
    11161 tagacctgcc attcggcacc taccagggta attcgcgcga agcgctggct tcagccataa
    11221 ggatcatgaa ggagacaggc gccagtgccg tgaagctgga gggcggccgt gagatagtgg
    11281 agtcgattga gcggatcatg tcagcgggca tacctgtgat gggtcacctg ggcctgacgc
    11341 cgcagtcgat tcacaagttc ggcacctacg ttgtcagggc ccgcgatgag gaagaggcca
    11401 ggaaactgaa ggaggatgca aaaataattg aaaaggcagg ttgttttgcc atcgtgcttg
    11461 agaagatccc tgccgcactg gcaggtgagg tggcagagtc actgaaggtg ccggtgatag
    11521 ggattggtgc cgggccgcag gtagacggac aggtactggt gctgcatgac atgctgggca
    11581 tcaatatgga gttttcaccg agattcctga ggagatacca taatctttac gatgagatca
    11641 agggggcggt acaccattat atcgctgatg tgaggtcagg caattttccc aacgagaaag
    11701 aacagtatta aaggatttgc gaattgcgaa ttgcgatttg aaggctaaaa gaatttaaat
    11761 aatcaggaag aaaaagatgc gagagggatt tccggaagta ctatacgaag acaatcacat
    11821 aatcatagtc aacaagcgtt catcggacct ggtgcagggt gacggcacag gggatgagtc
    11881 actggataat atagtaagag gatacattaa ggagaaatac ggcaaacccg gggatgtgtt
    11941 cctgggtgtg gtgcaccgtc ttgacaggcc ggtcagcggc tgtgtggtct acgcccgcac
    12001 gtcgaaggct ctcacccgcc tcagcgagct cttcaggacc cgtgatgtaa agaagagtta
    12061 ctgggccatt gtcacagacc ggccgcccgc cgaagagggt tcgcttcaaa actacctgaa
    12121 gaagaatgag aagcagaaca agtcatatgt gtatgaaaag gaggtaaagg gctctaaaag
    12181 cgctgaacta tcgtataaga tactgtcacg ttctgagagg tactacctgc ttgagatcga
    12241 tcttctcacc ggcaggcacc atcagatcag ggcgcagctg gctgccgcgg ggtgccccgt
    12301 caggggagac ctgaaatacg gtgccaggcg ttccaacgag ggtggcggca tatctcttca
    12361 ttccaggcga gtctctttca ttcacccggt aaggaatgag gagataaatg ttgtggcacc
    12421 tttgccagat gaccgcatct ggaagctctt taaaaacgtc tgattaaaga tggaatggag
    12481 ggctatgcct ccccggttag aaaacatgcc cggatacata aaagatgaag aggcgcctct
    12541 gccgcgcctc ttcccaactt aacctaaaac taacctaaat caaccacctg atgattgtcc
    12601 gggccctggc gggaccgtaa gaaatgctca tttcttcatc agtatctttt ttacctcact
    12661 cagtatcttg tcagcctcat ctgccttttc tttgggggct cttatactta caacaactcc
    12721 atccagggaa atgttgtaaa aaactccttc tttttcctcc tgcatccgga tcatggccct
    12781 gccgggcgct gctccggcag cctccccgcg ttccggaaaa tctgctgtcg tggcagtcca
    12841 taccatcctg ctccctttac cgggtttccg gatgattctt ttcccttcac ccgtttcgat
    12901 aatcaggttg ccaccctcat accggatggt gtcaccttca attactgctg agtcctttac
    12961 tatactgtct tcttccacag tgaccacaat caccttcttc tcgacattct ttgtctcctg
    13021 tgcggatagt gtagttgttc cggcaaagat ggctgccact attcctataa ccgttgagag
    13081 tgcttttgtc a
//
LOCUS       DAOPXU010000077        12868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_5379_length_12868_cov_14.266214, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000077 DAOPXU010000000
VERSION     DAOPXU010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12868
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5379_length_12868_cov_14.266214"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..90
                     /locus_tag="PKK03_08565"
     CDS             <1..90
                     /locus_tag="PKK03_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HNX84494.1"
                     /translation="NILRKKIDRDFEPKLIHTRIGLGYIFTDA"
     gene            91..1473
                     /locus_tag="PKK03_08570"
     CDS             91..1473
                     /locus_tag="PKK03_08570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX84495.1"
                     /translation="MKIRQKLTLQFFLMASVILLLFSLTVYILSATYRRDDFYQRLES
                     RAISTARLLIEVDEIDTDLLNKIEEDNPVSLPDEKIIIFNYKNEIVYSTDTRNVILYD
                     DDLLTRIRTSGQIKTKSGRYELMGILFPEEFNRFVVLSAARDIYGFDKMKNLRAILVL
                     LTGIFLLLFYPAGWLFAARSLSPISKVVDRVEEISIASLDLRVDEGNGKDELAHLAHT
                     FNRMLERLETAFEVQKDFISNASHELRTPLTAIRGQLEVLLMKDRTDKEYREATAAVL
                     EDISKLNRLLDRLLLMAHASSEGAREGFREVRIDEILWQAREELLKHEKEYTVTVALD
                     ENISDIENLTMRGDEQLLLSAVTNIMENGCKYSPDRSVRVLLSHDTNNILVSFTDNGP
                     GIAEDEIERIFESFYRSKSTSSVRGHGIGLSLASKIIDIHNGKITVTSRQGEGTTIRL
                     AFPAERFIMA"
     gene            1557..2477
                     /locus_tag="PKK03_08575"
     CDS             1557..2477
                     /locus_tag="PKK03_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84496.1"
                     /translation="MKRCLLIMLVLAGLSAGLAGQDRIITLRNDTIVCKITKITRNEI
                     QFDIVSQGVRAAGRLPLSEICSYSVSTLPMWEPGPAYPPAGTTPAGSLRIGFNGGMGY
                     LTSSSESAEETMAGVGIEEENARDYYDNLKSGWYGSADVAWIINQRYGVGVKYKFFNT
                     SAVTEGYFNLGDYYNLYYATYRENIYVNYGGLFLFYREPVGKKDLFSIYSAFSAGLTL
                     YRNESEVFSQAILITGKAPGLDGTFGLEYKITPLVSAGAEVSVFASKLRKIDITDGET
                     EQTQELDKEHHENLSRIEVSLGLRFYLWNR"
     gene            2474..4141
                     /locus_tag="PKK03_08580"
     CDS             2474..4141
                     /locus_tag="PKK03_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84497.1"
                     /translation="MKRTAYITGIILLTLAGVSCSDDFLEKNNAEWYSLSDTLIMDRQ
                     NYEATVMFELPEKINGDFTVFIRPVWLTVDDPTGDVREGKIEFKLSLDGTNLPSGYYS
                     HTGAVVLEIEDFGYVSFTVIFTDYGSPSIQCSPTELVFDGILTRTFTLTNPTPGILDW
                     TITEIPDWLAFSVDSGVLYQGQAEWITVTVDASKLVPGVGKNTTVRISSPHAQYPYML
                     KISVTPVTVPPPEGFTIGSILADAEYHHESGILAVCTSSPNQLILYNTGSWETDTIEL
                     DRAPGCISISEDGHTAVIGYSVASVSYIDLDAAAITDDFSIDCIPHDIVLGDNGWCYI
                     TPVGDQWEAMHSLDLNTGQLTVSTNISIVYEKTQIKKVPGKALMVGSRLTVSPTGLLL
                     FDIAAGLPKDEVTYYHESTGDFWISKDAARVYSAYGNVYTIPEFDGMNHGSTAPVYGM
                     FDLGYSYISTLDDCPAINSVFYSTAPSWHEEASSSLIEQYNSTSLIKTKSHNVSVAQV
                     SISGNLVPYETTPRFIFVNKEGTSMYVLKVIRPDYKIDGWLMETIAL"
     gene            complement(4185..4811)
                     /locus_tag="PKK03_08585"
     CDS             complement(4185..4811)
                     /locus_tag="PKK03_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84498.1"
                     /translation="MDKGTIGLNKETIRDQAVVRMSPRKLSLRDSGLTHLFNELDLEV
                     RQGLIDYLDRMGLVKESGMLVIPSSRHYFYDAEDMKGIRTVINLRQLNYIREIRDFLK
                     QITGLLPAKSNFIGCFIDNRAHSAGSDKNGSSSAQVSDKTEAYENGIESRIPFINRMY
                     SIIDFRTNRYLTRRAVTNLLKESGLELVGMTEINGFTYFHARKSNPAA"
     gene            complement(5076..5354)
                     /locus_tag="PKK03_08590"
     CDS             complement(5076..5354)
                     /locus_tag="PKK03_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNX84499.1"
                     /translation="MGLKVHYRIHVTGMVQGVGFRRSAAREAWKRGITGYVKNMADGS
                     VFIEAEGAREQLDEFSAWCRRGPGAGFVDNAEVTEGPPAGFWEFRIEH"
     gene            5473..7584
                     /locus_tag="PKK03_08595"
     CDS             5473..7584
                     /locus_tag="PKK03_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNX84500.1"
                     /translation="MKRGWKIAVVALGVIIFIPVFFISSVQAGIFGRLPGKKELSSFR
                     NATASVVLSHEGETIGRFFSENRITIPFDQMPPHLINALVATEDARFYEHSGVDSRSL
                     IRVVFKTILFSDRSAGGGSTITQQLAKNMYGRKDYRVFSVILNKTREVILAKRIEKVF
                     TKDEILNLYLNTVSFGENIYGIEAASQRYFSKKAADLKVEEAALLTGMLKANTMFNPR
                     LYPQNAVRRRNVVLGQMEKYGYLAGRAADSLSALPIKLNYSKTDLSGPADYFMVRVRM
                     EADRILKDISAYRGKEWDLERDGLVITTTLRLPLQQAAGEAFAAHMPRMQKRLDDQYS
                     TSSGRRALKEIPDSLKKMMTTLHAGLFAMDPATGAVMAWTGGIDFKTQPYDQVLARRQ
                     LASVFKPFIYAAALEDGVEPCTYLDNDSVTLSGFNDWSPENYNHSYGGKYSLAGALAQ
                     SMNIPTFSLFLMIGFDNIDRLWQQMGFSFPLVNMPSLAMGTAEASILEVARAYAAFAN
                     GGMLVTPYLIESVKAPDGRILWQYTPPRGSDRVLSERSATLMAAMLEKAIREGTGASV
                     HSVYGVKIPLAGKTGTSQSYADAWFAAFNPAMVIVSRVGASTPSIHFNSGSYGSGSAL
                     ALPLVAMTLKKAEKNKELMSVINTPFAPLPPELAMALDCPDFREKSFFDRLIDIFRDN
                     NIDYQKETRRRRIIRGRIFKI"
     gene            7655..8479
                     /locus_tag="PKK03_08600"
     CDS             7655..8479
                     /locus_tag="PKK03_08600"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HNX84501.1"
                     /translation="MKKKAILLLTAALILIAGACNEIRNGNDTGRVILKVTDAPFDVS
                     NIESALVTITKVELKKEGDGISDGYQYITLSEDTVILDLIDLRNGVAETLLDMEIPAG
                     RYDMVRLYIDEAGLKLKDSEEIHRVKVPGGSQTGIKIFLRPALEVAEGSLEEVLLDID
                     LSRSFILRGNPAHNNGFIFKPVVRAANMAKAGCVRGLVTDTAGLKINEASVWIEQDTV
                     LATAFTDTLGYYAIMGVPAGTWSVSATKEGYDTVRFDGVNIIAGNKTIQDFELTKK"
     gene            8633..10099
                     /locus_tag="PKK03_08605"
     CDS             8633..10099
                     /locus_tag="PKK03_08605"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNX84502.1"
                     /translation="MKKRRTLIYAALITAALVTVISCAVNPVTGRKQIMLMSEAQEVQ
                     LGLSYDPQVLASFGEYNSPALQSWVQAKGTEMGKLSHRPNLEYHVKVVDSPVVNAFAV
                     PGGYIYLTRGIMANLNNEAEFIGVLGHEMGHIAARHSVSQQTKQQLGTLLLIGGMIAS
                     PKFANYAEQAMQGLELLFLSFSREDEREADALGSEYATKMAYDGTKMADFYKVLIRMN
                     LSEAEGGVPTFLSTHPDPGDRYNAVLRNTKAWQDSLKLPSYKVNGDSYLQMIDGMIYG
                     EDPKQGYTMDNTFYHPELRFKFSYPLGWELTNAPTQVTVQPSDGRALMLFTLSNQKTL
                     QSAVDTTMAMYGLQLQRSEKKTVNGLQAIVTLAKQPVQDQSTGATGANAVISYFVQYG
                     SVIYVFHGVSTEADFASYAPTMEASMKTFAQLTDQSRINVKPKRVYVRKAPRAGTLAS
                     TLSALNMPQNLMEELSLLNNMELNETVPAGKYIKIIGE"
     gene            10124..11101
                     /gene="hflK"
                     /locus_tag="PKK03_08610"
     CDS             10124..11101
                     /gene="hflK"
                     /locus_tag="PKK03_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNX84503.1"
                     /translation="MAEQTFRKSGYRVWMDKNGLYLKYGSIALLVLILLATTLFQVQP
                     EEVGVITRFGKYVRKVEPGLNLKVPIIEKLYKVAVERQQKEEFGFRTTAPGVRSEYTK
                     EGTIDESLMLTGDLNLADVEWVVQYRIEDPYSFLFRVREPVVALRDISEASMREIVGD
                     RTVNEVLTVGRADIATSVQQKIQELCTEYSLGIRIEQVVLQDVNPPDPVKDAFNAVNQ
                     AQQERETLINQARSEYNKVIPRARGQAEETIQRAEGYATERVNNALGEASRFNALYLE
                     YVKAPEVTKRRIYLETMEDILPRAGNKIITDESGNNLLPLLQMQLTSKK"
     gene            11105..12055
                     /gene="hflC"
                     /locus_tag="PKK03_08615"
     CDS             11105..12055
                     /gene="hflC"
                     /locus_tag="PKK03_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNX84504.1"
                     /translation="MRTNTTRLIVTLSVLLVSLLLILECVYKVKETEQVVVTQFGKPV
                     GEAKTTPGLKLKMPFIQRANYFEKRYMEWDGDPNQVPTKDKKFIFVDTYARWQITDPL
                     QFFKRLTDERGAQSRLADILDGETRDHIAGHNLEEVVRSVNRTPASSGLIGEETGDTL
                     NNIVTGRQKIEDMILISANDKAKDLGIVILDVRIKRLNYVEEVRTQIYERMKSERYRI
                     AERFRSEGQGEASKINGEKERDLKNIQSEAYRKAEEIRGKADARAAAVYAGAYDQSAS
                     ARNLYGFLKSMETFRLTFDSTTTIILSTDSDLYRYLNGFN"
     gene            12213..12347
                     /locus_tag="PKK03_08620"
     CDS             12213..12347
                     /locus_tag="PKK03_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84505.1"
                     /translation="MKTYYQITAEGIGIVLLRKRKIAKALRRWLREKGLSYEYVFYIQ
                     "
     gene            complement(12704..>12868)
                     /locus_tag="PKK03_08625"
     CDS             complement(12704..>12868)
                     /locus_tag="PKK03_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HNX84506.1"
                     /translation="IQNIIDFLIVALCIFFVIKAMNSLKKKEEAAPATPPPPSEEVTL
                     LTEIRDLLKK"
BASE COUNT         3318 a         3290 c         3277 g         2983 t
ORIGIN      
        1 aacatcctca ggaagaagat agacagggac ttcgagccga agctcatcca cacccggata
       61 ggactcggat acattttcac ggacgcatag atgaagatac ggcagaaact taccctccag
      121 ttttttctga tggccagcgt catattgctg ctattctcgc tgacagtcta tatattgtcg
      181 gccacctacc gtcgcgacga cttctaccag cggctcgaga gcagggccat cagcacggcc
      241 aggctgctga ttgaagtgga tgagattgat accgacctcc tgaacaagat cgaagaggat
      301 aacccggtct cactgcccga tgagaagatc ataatcttca actacaagaa tgaaattgtg
      361 tattccactg ataccaggaa cgttattctg tatgatgacg atctgctgac gaggatcagg
      421 acatcgggac agataaagac gaagagcggc aggtatgagc tgatggggat cctcttcccg
      481 gaagagttta accgttttgt ggttctcagt gcggcccgtg acatttacgg gtttgacaag
      541 atgaagaatc tgagggcgat actggtactt cttaccggta tttttctgtt gctgttttat
      601 cccgcaggat ggcttttcgc ggcacggtcg ctctctccga tatccaaagt ggttgaccgc
      661 gtggaggaga tctccattgc cagccttgac ctgagggttg atgagggcaa cggaaaggat
      721 gaactggctc acctggcaca caccttcaac cgcatgcttg aaaggcttga gactgccttc
      781 gaggtgcaga aggattttat ttcaaatgcc tctcatgaac tgcggacacc gctgacagca
      841 ataagggggc agcttgaggt gctgctgatg aaggaccgta cggacaagga gtacagggaa
      901 gcaactgctg ccgtactcga ggatataagc aagctgaacc ggctgcttga ccgccttctg
      961 ctgatggccc atgccagctc cgagggggcc agggagggtt tccgggaggt cagaattgat
     1021 gagatactgt ggcaggcacg cgaggaactg ctgaaacacg aaaaggaata tacggttaca
     1081 gttgccctcg acgagaacat ctcagacatt gaaaacctta ctatgagagg cgatgagcaa
     1141 ctgctcctct cggcagtaac caacatcatg gagaatggct gcaagtattc ccctgacagg
     1201 tctgtaaggg ttcttctgtc acatgacacg aataacatac tcgtaagctt cacagacaat
     1261 ggtcccggca ttgcagagga tgaaattgag cgtattttcg aatccttcta ccgcagcaaa
     1321 tccacttcat cggtaagggg tcacgggata gggctctctt tggccagcaa gataattgac
     1381 atccacaacg gaaagataac cgtcacatca cgccagggtg agggaacaac catcagactt
     1441 gcatttcctg ccgaacgttt tatcatggct taaccaggat ataatctgaa aagttgttaa
     1501 ctttggtatc ggatgctctg catcagaagg tctgtattac aataaatatc tgcaatatga
     1561 agagatgtct gctcatcatg ctggtccttg ccgggctcag tgccgggctg gcagggcagg
     1621 acaggatcat taccctccgt aatgacacca ttgtctgcaa gatcaccaag atcacccgca
     1681 atgaaatcca atttgacatt gtcagccagg gggtgagggc agcaggacgt ctgccgctat
     1741 ctgaaatctg cagctactct gtttccaccc tgccgatgtg ggaaccgggg ccggcgtacc
     1801 cgccggcagg gacaacaccg gccgggagcc tgcgcatcgg cttcaacgga ggaatgggct
     1861 acctgacaag cagctcggaa tctgccgagg aaacgatggc aggcgtcgga atagaagaag
     1921 aaaatgcccg tgactattat gataacctca aatcaggctg gtatggcagc gctgacgttg
     1981 catggatcat caaccagaga tacggtgtcg gggtcaagta caaattcttc aataccagtg
     2041 ccgtaactga gggatatttc aatctgggcg actattataa cctctactat gccacctacc
     2101 gggaaaatat ctatgtcaac tacggggggc tgttcctgtt ctaccgtgaa cctgtcggga
     2161 aaaaggatct cttcagcatt tattcagcct tttcggcagg cctgaccctc taccgcaatg
     2221 agtcagaggt tttctcccag gctatcctga ttaccgggaa agccccgggc ctggacggaa
     2281 cctttggcct ggaatataag atcacgccac tggtttcagc aggagccgaa gtttcagtct
     2341 ttgcttcaaa actcaggaaa attgatatca ccgacggtga aacggaacaa acccaggaac
     2401 tcgataagga gcatcatgag aacctttcga ggatcgaggt ttcacttggc cttagatttt
     2461 atttgtggaa cagatgaaaa ggacagctta cattacggga attatcctgc tgactcttgc
     2521 aggcgtttca tgcagtgacg acttcctcga gaagaacaat gccgaatggt actccctttc
     2581 ggacaccctg attatggaca ggcaaaatta tgaggccact gtcatgttcg agcttcctga
     2641 aaagatcaat ggagacttta ccgtcttcat acgccctgta tggctgacag tggatgaccc
     2701 caccggcgat gtaagggaag ggaaaattga gtttaaactg agccttgacg gcaccaacct
     2761 tccttcaggc tattacagcc acaccggtgc agtggttctg gagattgagg attttggata
     2821 cgtaagcttt accgtcatct ttaccgatta cgggtcacca tccattcaat gctctcccac
     2881 ggagctggtg ttcgacggca tcctcacccg taccttcacc ctgacaaacc ctacaccggg
     2941 catccttgac tggaccatta cagaaatacc tgactggctt gccttttcag ttgattcagg
     3001 agtgctgtat cagggccagg cagaatggat aacggttact gttgatgcct cgaagctcgt
     3061 cccgggagtg gggaagaata caacagtcag aataagcagc ccgcatgcgc aataccccta
     3121 catgctgaag atatctgtta ctcctgtcac cgtacccccg ccggaagggt tcaccatcgg
     3181 cagcatcctc gctgatgctg aatatcatca cgaatcgggg atacttgccg tgtgtaccag
     3241 ttcgcccaac cagctgatcc tttacaatac cggctcatgg gagacggata ccatagagct
     3301 tgacagggct ccgggctgta taagcatctc cgaagatggg catacggcag taataggcta
     3361 ttccgttgct tcggtaagtt acattgatct ggatgctgcg gcaatcaccg atgacttcag
     3421 catcgattgc attccgcatg acatcgtgct gggagataat ggctggtgct atatcacacc
     3481 ggtcggtgac cagtgggaag cgatgcacag tcttgatctg aataccggac agctgacagt
     3541 cagcaccaat atttcgattg tttatgaaaa aacgcagata aaaaaagtgc cgggaaaagc
     3601 cctgatggtt ggctcaaggc tgacagtgag ccccaccggc cttctgttgt ttgacattgc
     3661 agccgggctg cctaaggatg aggttactta ttaccatgaa tcgacgggtg atttctggat
     3721 ctcaaaggat gccgcgcgcg tttattccgc ctacggaaat gtctacacaa taccggaatt
     3781 tgatggcatg aatcacggca gcaccgcacc tgtttacggg atgttcgatc tgggctactc
     3841 atacatcagc acgcttgatg actgcccggc gattaacagc gtcttctaca gcaccgcacc
     3901 ctcctggcat gaggaagcct cctcatcgct tatagagcag tacaattcaa cgagcctgat
     3961 aaaaacaaaa tcccacaatg tatctgtagc acaggtcagt ataagcggta accttgtacc
     4021 ctatgagaca acccccagat ttatcttcgt aaacaaagag ggtacctcta tgtatgtcct
     4081 taaggttatc aggccggatt acaaaataga cggatggctg atggagacca tcgcccttta
     4141 gaacattgct gaattcattg attgacgcac tctgatctgt gccttcaggc agccggattg
     4201 cttttgcgtg cgtggaaata agtgaagccg tttatctcgg tcatgcctac cagctcaagt
     4261 cctgactcct tcagcaggtt agtgacagcc ctcctcgtca ggtacctgtt tgtcctgaag
     4321 tcaatgatgc tgtacatcct gtttataaag ggaatccttg attcaatacc gttctcgtag
     4381 gcttcggtct tgtctgatac ctgcgctgaa gaactgccgt ttttatcaga cccggcgcta
     4441 tgtgccctgt tgtcaataaa acacccaatg aaattactct ttgcgggaag aaggccggtt
     4501 atctgcttca ggaagtcacg tatctccctg atatagttca gctgcctgag gttgatgacc
     4561 gtcctgatgc ccttcatgtc ttcggcatcg tagaaatagt gacgtgaaga aggaatgaca
     4621 agcatacctg actccttcac caggcccatg cggtcaaggt agtctatcag gccctgacga
     4681 acctcgaggt caagctcgtt gaacaggtga gtcagcccgc tgtccctgag cgacagcttg
     4741 cgcggactca tccttactac ggcctgatcc cttatagttt ccttattcaa cccgattgtt
     4801 cctttatcca tgtctgcgtc agatgccttc tctcaaacaa tttttgtgcc actatgtttt
     4861 gacagtatcc cttaagcagg attttcacac cgaagatatt cactcttcac catacgaacc
     4921 aaactatttt gatgttcgaa cctaaccctt tgaccaacgg tatcaaacag gggataatca
     4981 gcaaatagct gatccgtgac tgtcagcctg tcaataggtc aattcggatg taatttattc
     5041 tatatcttat agttcagtac gttttcaggt cattttcagt gttcaattct gaactcccag
     5101 aagcctgccg gaggtccttc ggtcacctcg gcattgtcaa cgaacccggc tccgggtcca
     5161 cgtctgcacc atgccgaaaa ctcatccagc tgttccctgg ctccttcagc ctcgataaac
     5221 acgcttccat cagccatgtt cttcacatac ccggtgattc cccgcttcca tgcctcacgg
     5281 gcggcgctcc ggcggaaacc caccccctgg accatgccgg tgacatgaat cctgtaatga
     5341 accttaagtc ccatgcctca tcggagtttg tcatgaagcg aatgaaccat aattctgttt
     5401 ttacctacaa tttaagttaa ttttgcacat tattaaaatg ttttatcaac aaaaagaggc
     5461 ctgaatccgc tgatgaaaag ggggtggaaa atagctgtcg tggcgcttgg ggtgatcata
     5521 ttcatcccgg tgtttttcat cagttccgta caggccggca tcttcggccg cctgcccggg
     5581 aagaaggaac tcagcagttt tcgtaatgcc acagcctcag tggtgctttc gcacgaagga
     5641 gagacaattg gcaggttctt ttcagagaac cggatcacca tccccttcga ccagatgcca
     5701 cctcacctga tcaacgcgct ggtggccacc gaggatgccc gcttctatga gcattccggg
     5761 gtggactcaa ggagtctgat cagggtggtt ttcaagacca tcctgttcag tgaccgcagt
     5821 gccggcggag ggagtaccat aacccagcaa ctggccaaga acatgtacgg ccggaaggat
     5881 taccgggtgt tttccgttat cctgaacaag accagggagg ttatccttgc gaaacggatt
     5941 gaaaaggtct tcacaaaaga cgagatactt aacctttacc ttaacacagt ttcgttcggc
     6001 gagaacattt acggaattga ggcagcctcg cagcgttatt tcagcaagaa ggctgctgac
     6061 ctgaaggtgg aggaggcggc cctgctcacc ggcatgctca aggccaatac catgttcaat
     6121 ccgcgtctct atcctcagaa tgccgtcagg agacgcaatg tggtgctggg gcagatggag
     6181 aaatacggtt acctggcagg cagggccgcc gattcgctca gcgcactgcc cattaaactc
     6241 aactacagca agacggatct ctcagggccg gctgattact tcatggtcag ggtaaggatg
     6301 gaggcagatc gcattctgaa ggatatatcg gcttaccggg ggaaagaatg ggaccttgag
     6361 cgtgacggcc tggtcatcac caccaccctg aggctgccgc tgcagcaggc tgccggtgag
     6421 gcgtttgccg cccatatgcc caggatgcag aagaggctcg atgaccagta cagcacatcc
     6481 tcgggacgcc gggccctgaa agagatacct gacagcctta agaagatgat gacaacactt
     6541 catgccggcc tcttcgccat ggacccggcc acaggagctg tcatggcatg gaccggtgga
     6601 atcgacttta aaacacagcc ctatgaccag gtgctggcca ggaggcaact tgcttcagtc
     6661 ttcaagccgt tcatatacgc tgccgcactt gaagacgggg tagagccgtg cacgtatctc
     6721 gacaacgact ccgtcaccct gtcaggattc aacgactgga gccctgaaaa ctataaccac
     6781 tcctacggtg gcaagtactc gcttgcagga gcactggcac agagtatgaa catacccacc
     6841 ttcagcctct tcctgatgat cgggttcgac aatatagaca ggctgtggca gcaaatgggt
     6901 ttctccttcc cgctggtgaa catgccttca ctggcaatgg gcactgcgga ggccagcata
     6961 ctggaagttg ccagggcata tgccgctttc gccaacggcg ggatgcttgt cactccgtat
     7021 cttattgaat cggtgaaggc gcctgatggc aggatacttt ggcagtacac gccgccacgc
     7081 gggtcagaca gggttctttc cgaacgttcc gccacactca tggcagccat gcttgaaaag
     7141 gccatcaggg aagggacagg agcctcggtc cacagtgtct acggtgtgaa gatcccgctg
     7201 gccggcaaga caggcacctc tcagagctat gctgacgcat ggttcgcggc tttcaatccg
     7261 gcgatggtta ttgtaagccg tgtgggcgcc tccacgcctt caatccattt caacagcggc
     7321 tcctacggtt caggcagcgc ccttgccctg ccgctggtgg ctatgactct taaaaaggcg
     7381 gagaagaata aagaattaat gagtgtcatc aacaccccct tcgctcccct gccccctgaa
     7441 ctggcgatgg cgcttgactg ccccgacttc agggagaaaa gcttcttcga caggctgatt
     7501 gacatcttca gggataataa tatcgactat cagaaggaga cccggcgcag gcggatcatc
     7561 cgcggcagga tttttaaaat ctgaaattca tcattccttc attctgcaca tcctactttc
     7621 gcacgagtag tgcaaaccat aaaacagaag caagatgaaa aagaaagcaa tcctgttgtt
     7681 gacagcggca ttgatcctta tcgctggagc atgcaacgaa ataaggaacg ggaatgatac
     7741 cggaagagtg attctgaagg ttactgacgc cccgtttgac gtaagtaata tcgaatcagc
     7801 gcttgtaacc atcacaaagg tagagctgaa aaaagaaggt gacggcatca gcgacggata
     7861 tcagtacatc acactttcgg aagataccgt gatccttgac ctgatcgacc tcaggaatgg
     7921 tgttgccgaa acactgcttg atatggagat cccggccggg aggtatgaca tggtgcgcct
     7981 ttacattgac gaggccggcc tgaaactgaa agactcggag gaaattcacc gcgtgaaggt
     8041 accaggcggc agccagacag gaattaagat tttcctgcgt ccggctcttg aagtcgctga
     8101 gggaagcctt gaggaggtgc tgcttgacat agatctctcc aggtcattca tcctcagggg
     8161 caatcctgcc cacaataacg gttttatctt taagcctgtg gtaagggcag ccaatatggc
     8221 gaaggcaggt tgcgtcagag gactggtaac tgatactgcc gggttgaaga taaacgaggc
     8281 aagtgtctgg attgaacagg atactgttct tgcaactgca ttcactgata ccctgggcta
     8341 ctatgcaatc atgggtgtgc cggccggcac atggtcagtt tcagcaacca aagaaggata
     8401 tgatacggtc cgttttgacg gtgtaaatat cattgcagga aataaaacaa ttcaggattt
     8461 cgagctcacc aaaaagtaac aggctgaata agtttgatat ggcttgaatt tgagccgtgc
     8521 ccctccggca cggcttttgt ttttgaataa ttaattccta aatttgggag attgcagcca
     8581 tgtttatggt gataacctaa acccgttaca aggaaaacat ttacacatta cgatgaaaaa
     8641 aagaagaact cttatttatg cagcgcttat taccgcagca ttggttacgg ttatatcctg
     8701 cgctgtcaac ccggttaccg ggaggaagca gattatgctt atgtcagaag cacaggaggt
     8761 tcagctggga ttatcatatg atccccaggt actcgccagt ttcggtgaat acaacagccc
     8821 ggcacttcag tcatgggtcc aggccaaggg aacagagatg ggaaaacttt ctcaccggcc
     8881 gaacctcgaa tatcatgtca aagtggttga ctcgcctgtg gtaaacgcct ttgcggtccc
     8941 cggaggttac atatatctta ccaggggtat aatggcaaat ctcaataacg aagctgaatt
     9001 cattggcgtt ctcggccatg agatgggcca catagccgcc cgccactcag tcagccagca
     9061 gaccaagcag cagctgggga cgcttcttct gataggcggg atgattgcct caccaaagtt
     9121 cgccaactat gccgagcagg caatgcaggg acttgagctg ctcttcctca gcttcagccg
     9181 tgaagatgaa cgggaggctg acgccctcgg atccgaatat gccacgaaga tggcatatga
     9241 cggcaccaaa atggccgatt tctacaaggt acttatcagg atgaacctct ctgaagccga
     9301 gggtggggtt cccaccttcc tttcgaccca ccccgatccg ggtgaccgct acaatgctgt
     9361 gctcaggaac accaaggcat ggcaggacag cctgaaactt ccttcctaca aggtaaatgg
     9421 agattcttat ctccagatga ttgacggtat gatctacggc gaggatccga agcagggata
     9481 caccatggac aacaccttct atcaccccga actcaggttc aagttctcct accctctcgg
     9541 atgggaactg acaaatgcgc ctacccaggt aaccgttcag ccttctgacg gaagagccct
     9601 gatgctcttc accctctcca accagaaaac cctgcagtca gctgttgaca ccacaatggc
     9661 aatgtacgga ctccagctgc agcgttcgga gaaaaagact gtcaacggac tccaggccat
     9721 cgtcaccctt gcaaaacagc cggttcagga ccagtcgacc ggagcaacag gtgcaaatgc
     9781 cgtcatatcc tatttcgttc agtacggatc agtgatatat gtattccacg gcgtctcaac
     9841 tgaagctgac ttcgcaagct atgcccccac aatggaagca tcgatgaaaa ccttcgctca
     9901 gctcacagac cagtcaagga tcaatgtcaa gcccaaaagg gtctatgtca gaaaggcacc
     9961 tcgtgcaggg acccttgcca gcaccctcag cgccttgaac atgccccaga acctgatgga
    10021 agagctttct ctcctcaaca acatggagct taatgagaca gtgccggcag gaaagtatat
    10081 caagatcatt ggtgaatagg caacggcaca aaactgacag acattggcag aacagacatt
    10141 cagaaaatca ggctacaggg tctggatgga taaaaacggg ctctacctga aatatggttc
    10201 cattgccctt ttggtcctca tcctccttgc tacaaccctc ttccaggttc agcccgagga
    10261 ggtgggtgtg attaccaggt tcgggaagta tgtgcgcaag gttgagcccg gactcaacct
    10321 caaagtaccg ataattgaga aactctacaa ggttgctgtg gagaggcagc agaaggagga
    10381 gttcggattc cgtaccactg ccccgggcgt ccggtctgag tacaccaagg agggaaccat
    10441 tgacgagtcc ctgatgctta ccggagatct caacctggca gacgtggagt gggtggttca
    10501 gtaccgcatc gaggatcctt acagctttct tttcagggtg cgtgagccgg tggtggccct
    10561 tcgtgatatc tcagaagctt ccatgcgcga gatagtgggt gaccgtaccg tgaacgaggt
    10621 tttaaccgtc gggagggctg acattgccac ctctgttcag cagaagattc aggagctctg
    10681 tacagagtac tcgctcggca tcaggataga acaggtggtg ctgcaggatg tcaaccctcc
    10741 tgaccctgta aaggatgcat tcaacgccgt caaccaggct cagcaggagc gcgagacact
    10801 catcaaccag gcccgttcgg aatacaataa ggtgattccc agggccaggg ggcaggccga
    10861 ggagacgata cagcgcgctg aaggctatgc taccgaaagg gtgaataacg cacttggcga
    10921 agcatcccgc ttcaatgccc tctaccttga atatgtcaag gcgcccgaag tgacaaagcg
    10981 gagaatttac ctcgaaacga tggaagatat ccttccccgt gccgggaata agatcataac
    11041 tgatgagagc ggcaacaacc tgcttcctct ccttcagatg cagctcacct caaagaagta
    11101 ggccatgcgc acaaatacaa caagactgat agtaactctc tctgtgctcc tggtatcact
    11161 gctgctcata cttgagtgcg tctacaaggt aaaagagacc gaacaggttg tggtgacaca
    11221 attcggcaaa ccggtcgggg aggcgaagac caccccgggc ctcaaactga aaatgccctt
    11281 cattcagcgt gccaattact tcgagaagag atacatggag tgggatggcg accccaacca
    11341 ggtgcctacc aaggacaaga agttcatctt cgtcgacacc tacgcccgct ggcagatcac
    11401 cgatccgctt cagttcttca agcgcctgac agatgagagg ggagcacaat cgaggcttgc
    11461 agatatcctc gacggtgaaa cccgtgatca tattgcaggc cataacctcg aggaggtggt
    11521 acggtcagtg aacaggacgc ctgcatcttc cgggcttatc ggtgaggaga ccggtgacac
    11581 gctcaacaac attgtgaccg ggaggcagaa aattgaggat atgattctga tctcggcaaa
    11641 tgacaaggca aaggatctgg ggatagttat ccttgacgtg aggatcaagc gccttaacta
    11701 cgtagaagag gtaaggaccc agatctatga gcgcatgaag agtgagcgtt accgtattgc
    11761 cgagcgcttc aggtccgagg gtcagggtga ggcctccaag atcaacggtg aaaaggagcg
    11821 tgatctgaaa aacatccagt ctgaggctta ccggaaggcg gaggagatac gaggcaaagc
    11881 cgatgcaagg gctgcggccg tctacgccgg cgcatatgac cagtcggcct cagcacggaa
    11941 cctctacgga ttcttgaagt caatggagac cttcaggctc accttcgact ctaccactac
    12001 catcatactg tcaactgaca gtgatcttta cagatacctg aacggtttca actgatgagt
    12061 tgtccttcct ccctgcgggg aaggaacaat cgatccatga tgatatcata acggggcccc
    12121 tgttggtcaa tacctggcca ttgtctgtca aaagaggctg gctgaaagct cttttccccc
    12181 gtatattagt atggaattca tccacggaaa tgatgaaaac gtactatcag ataacagcag
    12241 aaggaatagg tattgtattg ctccggaaga ggaagattgc caaggcactt cgcaggtggt
    12301 tgcgcgaaaa gggattgtcc tacgaatatg ttttttacat ccaataatct ttcactctca
    12361 ggttctgttt aatgattttt ccggagaaat ggagaccggc ccgggagggc cggtttttta
    12421 ttccaatgct ttaatccccc gacagcctga cggctatcgg ggcaagctct acagccaacc
    12481 accgctgcct gctgcctgaa atgactgccg actgccgact gccaactgta gaaaaggccg
    12541 cactcttgcg aatgcagcct ctgttcaatg tctgttgctg tgagccgttc tagcccctgt
    12601 cagggaagaa cctgcgccac cagtaatatt tccttattct tgccatagta aaatcgtctg
    12661 caaatatgca aacaattttc ggcagcaaat attgtaccac tgactatttt ttcagcaggt
    12721 cacgaatctc cgtcagaagg gttacttctt cgcttggcgg cggcggagtt gcaggagctg
    12781 cttcttcctt cttcttcagg ctgttcatcg ccttaatcac gaagaatata cacagggcca
    12841 caatcaggaa gtcgatgatg ttctgaat
//
LOCUS       DAOPXU010000078        12557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_5600_length_12557_cov_12.535034, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000078 DAOPXU010000000
VERSION     DAOPXU010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12557
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5600_length_12557_cov_12.535034"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PKK03_08630"
     CDS             complement(<1..784)
                     /locus_tag="PKK03_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4968 domain-containing protein"
                     /protein_id="HNX84507.1"
                     /translation="MILIMKRCLVLLIVVSTVCPISSGQSYSRTDLGIRTAIDGIGIE
                     IQFYSPSIVRIVKWPEGKSFSKESLSVIMTPQKTDLGLKQKGDELIARSKTIAVSLNL
                     RNGTISFMTAGSGLLLSESEAGAGFTGFNDAGTETYSAYQAFVLDKNDAIYGLGGQQD
                     GKMIRRNVTLNMIQGNTDDYIPFFVSVKGYGLFWDNCSPTVFADTPEFTSFKSDVGEC
                     IDYYVMYGGSADGVIARMRDLTGQVPMFPLWTFGYWQSRERYR"
     gene            complement(854..1570)
                     /locus_tag="PKK03_08635"
                     /pseudo
     CDS             complement(854..1570)
                     /locus_tag="PKK03_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666410.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
     gene            complement(1733..2893)
                     /locus_tag="PKK03_08640"
     CDS             complement(1733..2893)
                     /locus_tag="PKK03_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNX84508.1"
                     /translation="MKKIALFLFAMMICGIASAQFAGGMRGQQQEPLPDGYKPSSTNT
                     FLAQYPAVNAQTRQAIFKVVAPHADNVALDLAGKKYSMTKDEKGVWTVISDPQVVGFH
                     YYAILIDSVPVMDRNTDSFFGSNWESSGIEIPEGPEGDYYRFNKSIPHGQVRSLYYWS
                     EINGMERHCNVYVPAEYESNTDKKYPVMYLLHGWGEDENGWSNQGHMANIMDGLINAG
                     KAVPMIVVMDCGDIKTNSDVRKASTNDVTQIYINDLMPFIEKTFRTKNDRQNRAMAGL
                     SRGGFQTTQTVFANLDKFAWMGTFSGFFSRPGQDVTETFNGVFKDPAAFDKQMNLLFI
                     STGTEERTPKEAVDALKAHGIKNIVYHESQGTAHEWLTWRRALNEFAPRLFK"
     gene            complement(3018..3461)
                     /locus_tag="PKK03_08645"
                     /pseudo
     CDS             complement(3018..3461)
                     /locus_tag="PKK03_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007411427.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
     gene            complement(3499..5025)
                     /locus_tag="PKK03_08650"
     CDS             complement(3499..5025)
                     /locus_tag="PKK03_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNX84509.1"
                     /translation="MKHLNLFMSIVAVSATIGMTSCNAKRQAYLPDLESLKKVNPAPE
                     WFKDAKFGIYFHWGVYSVPAYANEWYPRNMYMERSRENAHHKEVYGAPADWPYHNFIT
                     GANDLKGNYVQFKPRLKSEGGAFDPDEWAQLFADAGAKYAGPVAEHHDGFSMWASKVN
                     PWNAKDMGPQLDLAGLLTDAIRKQNLKVIMSMHHAFNITGFYDAVPQRDDPVLQILYG
                     QQGTEKNVALWSAKLKEIIDVYKPDIIWQDFNLHKIPENALLGFLAYYYNKASEWNKE
                     VVATYKDGLNTECAVLDFERGGPADITEKYWLTDDAISSSSWCYTEGIGYYSPKQVLH
                     GFLDRISKNGNLLLNISPMADGTIPQEQKDILLAMGAWLKKYGEAVYATRTWEKYGEG
                     PTLMGAGQGVFTEPAEGTAMDIRYTQSKDNKTLYAIMLGWDKGQKGITLTSLAPNNVD
                     LRTLKSVTLINAEAGKYLPLKYSQNPEGLTISLPDRSFEEMAYVIRLTFRRKMETTRQ
                     "
     gene            complement(5126..6337)
                     /locus_tag="PKK03_08655"
     CDS             complement(5126..6337)
                     /locus_tag="PKK03_08655"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNX84510.1"
                     /translation="MEKVRHPWPWWRWILAGLNTLALALTIIMSWHYLAGGSVAGCGG
                     GSPCEKVLGSRWSEIADILPVSGLALGVYLSMLVAGFFTGPASELPVRRMAWGAMLIM
                     AGAVAGSAVWFTVIQKWIIGAFCPYCLAAHLTGMLLSVLIIWRSLKVADHGGLKNDTP
                     ASGKRLFRPVHVTGLVLAGMFAAGLLAMTQVISAPSAAYTDTVSQDNLPEVDYNAVPM
                     TGSPDAPYIVSLLFDYQCTHCQQLHFMLDEAIRIYSGRLAFMLCPTPLDPGCNSFIPP
                     GRDEYRNSCELAKISLAVWRGRREAFPEFENWMFTFETGDRWYPRSPEAARAKAIELL
                     GPEGFDAAWSDPWIEEYLRTSIGIFGKTLQSGRGGIPKLIYGSRWVIPESFSAGDLVM
                     ILRKSLMVPEP"
     gene            complement(6337..9675)
                     /locus_tag="PKK03_08660"
     CDS             complement(6337..9675)
                     /locus_tag="PKK03_08660"
                     /inference="COORDINATES: protein motif:HMM:NF020768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0031221 - arabinan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinofuranosidase catalytic domain-containing
                     protein"
                     /protein_id="HNX84511.1"
                     /translation="MRKKSSFVVILALALALPVTLMMVITVAGTGCNVSSARPEGPCD
                     IYAAGGAPCVAAHSSTRALYAKYNGPLYQVMRQSDGRTLDIGVVRGGKGDPGGYANAA
                     AQDKFCANTYCWITILYDQSGKGNHLTQAPRGGFSGPAMGGFNNLPIADMAPVTVMGH
                     KVYGIFIEPGMGLRDDDPVGTAVDDQAEGQYWVINGLHYNSGCCFDYGNAETDSRDDG
                     DGTMETTYYGNATAWYTGQGTGPWIMTDQENNLVGCVNPSPDDKYCPDLPSITWRFVT
                     ATADGEPHHWRAMGGDAQQGELKTMFDGPRIINDRSSYDPMRKQGAILLGNGGDNSVG
                     SQGTFYEGAMTAPGTFPTNETNQKIQANIVAAKYDVQRLSIAPADQIDTPPGLQTFAP
                     GSTRKTTVSFTNTTGAPVSNLNLSIIAPKGWKTVLTDSEESSMTFTNTIAPGERVSAT
                     FEVTSASEIYNGDLTGQASWMAQGNSKSRSETAVEKVRNVSPVKINEFRVSDGSPENS
                     TNSFIELYNASDTETDISGWTLTHHQTMMPAFSSVKIPAATKLAPKGFYLLGLSTSGL
                     AVPANKGENVLYVRSAAGMSAGDIVRIGTGTAEETRTIASVTIPPAPQAPANPFGFGR
                     RGPAGQTTVWQPLPDGPVITIPAGSTNIPVTGVDGFEVGQKMAIGYGATYPAVAMAVE
                     KYEVVTITAVGKPGTQAWLSMDARAGDKNIKVSSVENISVGDKIRLDIESEGHGIETV
                     TVTRVGTQSSRSTFNGPLTDKDDPGTGLDLAEPLKFNHASNMPFSVRGTGISFEPATA
                     AAHSSNEPVLALRHIITLDQPLAYNHETDDVVSDEKVTSSGYQGARKPDQWFGGPALS
                     ASAGNMVLRDAANIVVDALNYGGLVDPWAAEGYQAASGANESGCFVPSPSAIRGFWMG
                     PAMTMTQPNRSAGRYPDGTDMDSNCRDFMIQNTVSLAARAASGSENIKVTSVAGFSND
                     QSIIIGSGAASETAVIATVGTSGATTTEAATAAGRNIIRVGTVAGFSAGQTITIDDGS
                     RNETAVIAAVGAARRRFGPQATTQTDSITVSAPLRYAHARGVQVSGSGITLVKPLKMD
                     HDNGGQIASNLPTPGEPNQYVRKP"
     gene            complement(9729..>10290)
                     /locus_tag="PKK03_08665"
     CDS             complement(9729..>10290)
                     /locus_tag="PKK03_08665"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HNX84512.1"
                     /translation="NPNFLQQQNSQRDAIVAALNFNIFARHAERVHGANIAQMINVLQ
                     AMIFTDGNKMVLTPTYHAFRMYVPFQDAVRLPVNFNAGSYQVEGIDLPRLDAIAAKGK
                     DGNIYVAITNIDPNNPASIELPLDGYSIVSVKGETLYTSSIDAVNTFESPENVSPKEI
                     DADVTGSKVSVTVHPQSLTVLSIAVK"
     assembly_gap    10291..10390
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10391..11488)
                     /locus_tag="PKK03_08670"
     CDS             complement(<10391..11488)
                     /locus_tag="PKK03_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HNX84513.1"
                     /translation="MNINLKRLIVLQGMVALVFLFSMCSERGKEQGNDTTVVIKIDPS
                     QTGPVINRNIFGQFAEHLGFGIYGGIWVGPESDIPNTRGIRNDVVEALKALKVPNVRW
                     PGGCYADKYHWRDGIGTPEERRARINVSWGGAPEPNTFGTHEFFDFIEQIGSEAFISA
                     NVGSGTVKESADWLEYLTASGSALAAERARNGHPEPYNVAFWGVGNEVWGCGGPFTPE
                     EYITELKKFATFSTNYNTDVNTQYVAVGPDSYDQYSFGYAEPIMKAWANKTWTWNIHG
                     LSLHNYTRGGWPPVIKATGFDELQYASVLEETLRMDKFVTENRALMDKYDPESKVSIL
                     VDEWGTWYAPSEGTNPNFLQQQNSQRDAIVAA"
     gene            complement(11631..>12557)
                     /locus_tag="PKK03_08675"
     CDS             complement(11631..>12557)
                     /locus_tag="PKK03_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84514.1"
                     /translation="SGITLESALEKNHEAGAPVNNPLIASAGYQGPVKPDQLYGMPLS
                     PASGSIALMDGSGNLVVDAMVYGSQQSNSSANGTIPSPELATLEGDQSQGGCMVVVPG
                     SGFSGQGMALRSTSMVNRSLGRFPDGNDTDSNCDDFLLQDEATLLLPATAGSNNIKVG
                     SVTDLKAGQRVFIGSGSDIESMVIEAIGTSGGTTVISDVNAGATAIPVAGIEGFSEGQ
                     TITVGSGTNIETAVVASVTSARGRPGVQGAVPAQSVTVTSPLTKSHVAGALVTGSGIT
                     LASPLKLNHDRGSQVASNVPTPGKTNQYAREQ"
BASE COUNT         2863 a         3224 c         2984 g         3386 t
ORIGIN      
        1 tcctgtaacg ttccctgctt tgccagtagc cgaatgtcca caacgggaac atcggcacct
       61 ggccagtcag gtcgcgcatg cgtgcgatga caccatcagc actgcctccg tacatcacat
      121 agtagtcaat gcactcaccc acatctgatt tgaatgaggt gaactcaggt gtgtctgcaa
      181 agaccgtagg tgaacagtta tcccagaaca ggccgtaacc ttttaccgaa acgaagaaag
      241 gaatataatc atcggtattg ccctgtatca tattcaatgt cacgtttcgc cggatcattt
      301 ttccatcctg ttgcccgccc agaccgtata tggcatcatt cttatccagg acaaatgcct
      361 ggtatgcact gtatgtttca gtacctgcat cgttgaaacc ggtaaacccc gcacctgctt
      421 cactttcgct cagcaataag ccggatcccg ctgtcatgaa tgatatcgtt ccgtttctga
      481 ggtttaatga taccgcaatg gttttgcttc tggcaatcag ctcgtctcct ttttgtttta
      541 aacccagatc agttttctga ggggtcatta ttaccgaaag actctccttg ctgaaactct
      601 tcccttccgg ccatttaacg atccttacta ttgacgggct gtagaattgg atttcaatcc
      661 caatgccatc aattgcagtt ctgatgccca ggtcagtcct gctgtatgat tgtccggagc
      721 tgatcgggca gactgttgaa accacaatca ggagtaccag gcagcgcttc atgatcagta
      781 tcatatgcgg gtttaaactg atgctgtcag tgtaagttta tttcagtccg tgttaaggca
      841 tattgatatt atatcccatg agagaaagca tcctggcagc gtaccttctt ccgagaatac
      901 gataaccttc cggtgtgaaa tgaaggtgat caggcattcc gggacaaccc tcggaggata
      961 tcacccagga gttcggaata acctgcggaa gggtggcaat tatagcgttc atatcggtac
     1021 atattccacc ttcggcagca ctaaccattt caccggcaag gaaaggcacc ttctcaggct
     1081 ggagaccaag gtcgttcagc aggttttcat agaccttgcg gactttgccg ggccatgtgg
     1141 tatcgccatt gtctgactca ccctgatgca tgagaattcc ctttatgacg ccatcctgtt
     1201 gggccagttt tgccatctca acaagccggc cataggggtc accatcgtag gagttgatca
     1261 tcgggaccat ccatcgtggt gcagttgaga catattcctt gtaagtctcc ttgtcaaaca
     1321 gctgtatttt gcagcccccg atggaaacat tgatgactcc gaccctgata ttcttcggga
     1381 ggttttcaac cattgtcctg ccgaaataat cggcaggtga caatccggtc ctgcacctgg
     1441 taagaggcgg gatggccgga taccagcctc ccttgactct tccgaggttc gggcagtcaa
     1501 tggtttccat aaccatgaac cttccgtcca ctgtggtgtc ctgaggctca atcctggcag
     1561 ctccttccat gtttgactgg ccgaagcaca ggtatatgtg gaaatccgga tcctgtgcaa
     1621 cgaccctggc acctgacagg aagagagtga caataaccag aaacaatagt tttgttttca
     1681 tattttattt tgagaattag cgctaccgcg aaagctagtg aaaaatttcc cgttatttga
     1741 ataatctggg agcaaattca ttcagtgcac ggcgccaggt aagccattcg tgggctgtgc
     1801 cctgtgattc gtgataaacg atgtttttaa ttccatgtgc cttaagggcg tcaactgctt
     1861 ctttcggcgt gcgttcttcg gtgccggtac ttatgaacag gagattcatc tgtttgtcaa
     1921 aggctgcggg atccttgaaa acgccattga aagtctctgt aacgtcctgg ccgggacgtg
     1981 aaaagaaacc gctgaatgtg cccatccatg cgaatttatc caggttggca aaaacagttt
     2041 gggtggtctg gaacccgcct cttgacagac cggccattgc gcggttctgc ctgtcattct
     2101 ttgtgcggaa agttttttcg atgaatggca tcaggtcatt gatatatatc tgcgtcacat
     2161 cgttcgttga ggctttgcgt acatcagaat tggtcttgat atcgccgcag tccataacta
     2221 ctatcattgg aacagctttg ccggcattga tcaggccatc cattatattg gccatgtgtc
     2281 cctggtttga ccagccgttt tcatcctcgc cccagccatg gagcaggtac attaccggat
     2341 atttcttgtc agtattgctt tcgtattcag caggcacgta aacattgcag tgacgctcca
     2401 tcccgtttat ctccgaccag tagtacagtg agcggacctg tccgtgagga atgcttttgt
     2461 tgaacctgta gtaatcacct tcgggacctt cgggtatctc tatccctgat gattcccagt
     2521 tgctgccgaa aaacgagtca gtgttcctgt ccattaccgg aacgctgtcg ataaggatgg
     2581 catagtaatg gaagcctaca acctgagggt cagagataac tgtccatact cctttttcat
     2641 ccttcgtcat gctgtatttt ttcccggcaa gatcaagagc cacattgtcg gcatggggtg
     2701 ccaccacttt gaagattgcc tggcgtgtct gagcatttac agccgggtac tgggcaagaa
     2761 aagtattggt tgatgaaggt ttgtaaccgt caggaagggg ttcctgctgc tggccgcgca
     2821 tacccccggc aaactgggca gatgcaatgc cgcagatcat catagcgaac aaaaagagtg
     2881 caattttttt catgattttt tcagtatttg agtttataaa cgattcactt taatatcacc
     2941 tgatcagtat gtttacagcc ttcagatcct tctcatatga actgttcccg taaagaatct
     3001 cgtattttcc cggggtcacg gccccaccgc ggatcacgga agatattgat gtttggcgtc
     3061 cagacggaaa ggctgaggaa acgtctgttc tcctgccctc tctgaattgc ctggttgtat
     3121 ttggcacgtg tctcatccga cactgcgctg aagattctgt atacaagctc atcgtcaaat
     3181 gaggcagcca tgccgacggg ctggggaaaa acagttacgc tgtcattgtt ggccaggcca
     3241 tgcaatgcct cgctccacca gttgaatgtc ttaatgccca ggcgcggtat ggcctctgac
     3301 ttgtcacaca tcagaagggc cttctcttca atggtcagcc ggcttataag atcttttgcc
     3361 cgctcctcgg aattcagggc ggggttctga aacgggtact gttgtgctga taaggaggag
     3421 gcaagtacca gattcaggac aatgattacg gttcttctca tattcttctc atttcttgtt
     3481 tctgtcaggt cttcaggttc actggcgggt tgtttccatt tttcttctga aggtgagtct
     3541 gatcacgtat gccatttctt caaaagacct gtcaggcagg ctgatcgtca gcccttccgg
     3601 attctggctg tatttaaggg gaagatattt tcctgcttct gcattaataa gtgtgacgga
     3661 tttcagggtc ctgagatcaa cattatttgg ggccagcgaa gtaagggtta tccctttctg
     3721 gcccttgtcc cagcccagca tgattgcata cagagttttg ttatcctttg attgtgtgta
     3781 tctgatatcc attgctgtgc cctctgccgg ttctgtgaat acaccttgcc cggctcccat
     3841 cagggtcggt ccctctccgt atttttccca ggttcgggtg gcatagacag cctcgccgta
     3901 ttttttgagc caggcaccca tggccagtaa aatatctttt tgttcctgcg gtatcgtacc
     3961 gtcagccatc ggggagatgt ttaagaggag gttgccgttt ttgcttatcc tgtcgagaaa
     4021 accgtgaagc acctgttttg gggagtaata cccgattcct tcagtataac accagctgga
     4081 agaactgatt gcatcatcgg taagccagta tttttcagta atgtcagcag gaccgccacg
     4141 ttcgaagtcc aggactgcac attcagtatt gagaccgtcc ttatatgttg caaccacctc
     4201 cttattccat tccgaggcct tgttataata atatgccagg aacccgagga gcgcattctc
     4261 cggaatttta tgaaggttga aatcctgcca gataatgtca ggcttgtata catcaattat
     4321 ctctttaagc tttgctgacc acagagctac gttcttttct gtaccctgct gtccgtaaag
     4381 aatttgcagg acggggtcgt ctctctgtgg caccgcatca taaaagccgg taatgttgaa
     4441 ggcgtgatgc atcgacatta tcactttcag gttctgcttt cttattgcat cagtcaacag
     4501 gcctgcaagg tcaagttgag gacccatatc ctttgcattc cacggattga ccttacttgc
     4561 ccacatggag aagccgtcat gatgctctgc aaccggtccg gcatatttcg ctccggcatc
     4621 agcgaaaagc tgagcccatt catcagggtc gaaagcacct ccctcagatt tgagcctggg
     4681 tttaaactgt acataattac ccttcaggtc atttgccccg gtgatgaaat tgtggtaagg
     4741 ccagtcggca ggtgcaccat aaacctcttt atgatgcgca ttctccctag atctttccat
     4801 gtacatgttg cggggatacc attcgtttgc ataggcaggt acagagtaga ctccccagtg
     4861 aaaatagatt ccgaatttgg catccttaaa ccactcggga gcaggattga ctttcttcag
     4921 cgattcgagg tcaggcagat aggcttgtct cttcgcatta cacgaagtca tcccgattgt
     4981 cgcggaaact gcaacgattg acatgaacag attcaggtgt ttcataatac attcaggtat
     5041 ttttgtcaag gtcctgttcc gtggttaagt ttctctttat aatgttgctt cattattatc
     5101 agatttgcca ttctgtctcg ctgttttagg gttcaggaac catgaggctc ttcctgagga
     5161 tcatgacgag gtcgccggcg ctgaatgatt cgggtattac ccatcgtgac ccgtagatca
     5221 gcttcggaat gccgcccctg ccgctctgta aggtcttgcc gaagataccg attgaggtcc
     5281 tcagatactc ttctatccag ggatccgacc aggcagcatc gaatccctcc gggccgagca
     5341 gttcgattgc ttttgccctg gcagcttccg ggcttctggg ataccacctg tcaccggtct
     5401 caaatgtgaa catccagttt tcaaattcag gaaacgcctc acgccttcct ctccatactg
     5461 caagactgat ctttgcaagc tcgcatgaat tcctgtattc atccctcccg ggaggtataa
     5521 aggaattgca tcccggatcc agtggagtgg ggcagagcat aaaggccagc ctgccgctgt
     5581 aaatccggat tgcctcatca agcatgaaat gcaactgctg acaatgggtg cactgataat
     5641 caaagagcag tgagacaatg tagggagcat caggagatcc ggtcattggg acggcattat
     5701 aatcaacttc aggcaggtta tcctgtgaca cagtatcggt gtaggcagct gacggagctg
     5761 aaatgacctg ggtcatcgcg agaagaccgg ctgcaaacat cccggccagg accagtcctg
     5821 tcacatgtac tggccggaac agacgctttc cggaggccgg agtatcattt ttgagacctc
     5881 cgtgatccgc aaccttcaat gaccgccaga taatgagaac agaaagcagc ataccggtta
     5941 aatgtgcagc caggcagtaa gggcagaaag ctccgatgat ccatttctgt atgacagtga
     6001 accatactgc acttcccgca acagcgccgg ccatgataag catagcgccc caggccattc
     6061 gccgtaccgg cagttctgat gccggtccgg taaaaaaacc tgcaaccagc attgaaagat
     6121 aaacccccaa agccagacca ctgacaggca atatatctgc aatctccgac caccggctac
     6181 cgagcacctt ttcacacggg ctcccgccac cgcagccagc caccgatccg ccggcgagat
     6241 aatgccagct catgattatt gtcaatgcaa gtgccagcgt attcaatccg gcaaggatcc
     6301 aacgccacca gggccaggga tgtcgtactt tctccactaa ggtttccgga catactggtt
     6361 aggctcaccc ggtgtgggga ggttgctggc tatctgaccg ccattgtcat ggtccatttt
     6421 caatggcttt acgagagtaa tgccgctgcc ggagacctgt accccccttg catgagcata
     6481 tctcagaggt gcggagaccg tgatggagtc tgtctgtgtg gttgcctggg ggccaaaacg
     6541 gcgccgtgcg gctccaacgg cagcaattac tgctgtttcg tttctgctgc catcgtcaat
     6601 tgtaattgtc tggccggcac tgaagcctgc aactgtccca acgcggatta tatttcttcc
     6661 tgctgcagtg gcagcttctg ttgttgtagc tcccgaggta ccaacagttg ctataacagc
     6721 agtctcgctg gcagcgcctg agccgataat tatactctga tcattgctga agccggcaac
     6781 acttgtgact ttgatattct ccgaaccgga cgctgccctg gcagcaagtg aaacagtatt
     6841 ctgaatcatg aaatcacggc aattgctgtc catgtcagtg ccgtcgggat atcggcctgc
     6901 gctccggttg ggttgcgtca ttgtcattgc aggtcccatc cagaaacctc ttatggcaga
     6961 aggtgatgga acgaagcatc cgctctcatt ggctccggaa gcggcctggt acccttcggc
     7021 ggcccacgga tcaaccagtc cgccgtaatt gagagcgtca acaacaatat tggcagcgtc
     7081 acgcagcacc atatttcctg cgctggctga cagtgcaggt cctccgaacc actgatccgg
     7141 cttccttgct ccctggtagc cagagctggt tactttttca tctgatacca catcatcagt
     7201 ttcgtggttg taagccagcg gctgatcaag ggtgatgata tgcctgagag ccagaacagg
     7261 ctcgttgctt gagtgagcag cggctgtagc cggttcaaag ctgatacccg tgcccctgac
     7321 actgaagggc atgtttgatg catgattgaa tttcagcggc tcagccaggt cgagtcctgt
     7381 tccgggatca tctttatccg tcagaggccc gttgaatgtg ctgcgcgacg attgtgtgcc
     7441 gacacgggtt acagttacag tttcaatccc gtgtccctca ctttcgatat ccagccttat
     7501 cttatctcca acggagatgt tttctactga tgataccttg atatttttat ctccggcccg
     7561 ggcatccatt gaaagccatg cctgggtgcc gggcttgccc acggcggtta ttgtaaccac
     7621 ctcgtatttt tcaacagcca tggcaactgc cgggtatgtt gccccgtaac ctatagccat
     7681 cttctgaccg acctcaaaac cgtcaacacc tgtcacaggt atgttggtcg atccggcagg
     7741 gattgtgatc acaggaccat caggaagagg ctgccataca gttgtctgac ctgcagggcc
     7801 acggcgtccg aaaccgaatg ggtttgccgg agcctggggt gccggcggaa ttgtcacact
     7861 cgcaatcgtg cgggtctcct ctgccgtacc ggttccaatc ctgacaatat caccggccga
     7921 catccctgct gcactcctga catagagtac gttctctccc ttattcgcgg gaaccgccag
     7981 gcctgatgtt gaaaggccca gcaggtaaaa gcctttcggg gcaagttttg tcgcggccgg
     8041 aatcttaact gatgaaaagg ccggcatcat tgtctgatga tgtgtaagag tccagcctga
     8101 gatatcggtc tctgtgtcac ttgcattgta aagctcgata aatgagttgg tggagttttc
     8161 aggtgagccg tcgcttactc tgaactcatt aattttaacc ggactgacgt tacgtacctt
     8221 ttcaacggct gtttcagacc gggatttgct gtttccctgt gccatccatg aagcctggcc
     8281 tgtaagatcg ccattgtata tttctgatgc ggaagtgact tcgaaggttg cgctcacgcg
     8341 ttcacccggg gcaattgtat ttgtgaaggt cattgaggat tcttcagaat ctgtaagcac
     8401 agttttccag ccttttggcg ctatgatgct gagattgagg ttactcacag gggctcctgt
     8461 agtgtttgta aatgaaacag tggtcttccg tgttgacccc ggtgcaaatg tctgcagtcc
     8521 cggaggtgta tcaatctgat cagcaggtgc aatactcaga cgctgaacgt catacttagc
     8581 tgccacgatg ttggcctgga tcttttgatt ggtctcattt gtcggaaatg tacccggggc
     8641 tgtcattgca ccctcataga aggtaccctg tgaaccgacg ctgttgtcgc cgccgttgcc
     8701 gagcagaata gctccctgct tgcgcatcgg atcatagctg ctgcggtcat taattatacg
     8761 tggtccgtca aacatcgttt taagttctcc ctgctgagcg tctcctccca ttgctctcca
     8821 gtggtgaggt tcgccatctg ccgtggctgt aacgaaccgc catgttatac tgggaagatc
     8881 cgggcagtat ttatcgtccg gagacggatt gacacaaccc accaggttgt tctcctggtc
     8941 tgtcatgatc catggaccag tgccctgtcc ggtataccag gcagtggcgt tgccgtagta
     9001 ggtggtttcc attgtaccgt cgccgtcatc gcgactgtct gtttcggcat tgccatagtc
     9061 aaagcagcag cctgagttgt agtgaagtcc gttgataacc cagtactgac cctctgcctg
     9121 gtcgtctacc gcagtgccaa ccgggtcatc gtcgcggagg cccatacccg gttcaatgaa
     9181 gatgccgtat accttgtgac ccatgaccgt gaccggcgcc atatcggcta tcggaagatt
     9241 attaaatccg cccatggcgg gaccgctgaa accgcccctt ggtgcctggg tcaggtggtt
     9301 ccccttgcct gactggtcat agaggattgt aatccagcaa tatgtattgg cgcagaattt
     9361 atcctgagct gctgcattag cataacctcc cggatcaccc ttgccaccgc gcaccacacc
     9421 tatgtcaagc gtcctgccgt cagactggcg cataacctgg tacagcggac cgttgtactt
     9481 tgcatacaga gcacgggtgc tgctgtgagc ggcaacgcac ggagcacctc ctgcagcata
     9541 gatatcacac ggaccttccg gtctggcaga agaaacattg cacccagtac ctgctacagt
     9601 tatcaccatc attagtgtga ccggcagagc aagtgccaga gccagaataa ctacaaaact
     9661 gcttttcttt ctcatgttat gtccattgac ctgatatgat gattatttga aatagaaatc
     9721 aaaatacttt attttacagc aattgacagt accgttaatg attgcggatg cacggttacg
     9781 gacactttgc tccctgttac atctgcatcg atctcttttg gtgaaacgtt ctccggactt
     9841 tcaaatgtgt taacagcatc gattgaggag gtatagagcg tttccccttt cacactgacg
     9901 attgaatagc catccagggg taattctatc gaagcagggt tattcggatc gatattggtg
     9961 attgccacgt aaatgtttcc atctttccct tttgcagcaa ttgcatcgag ccgcggaagg
    10021 tcaatgcctt ccacctggta actgcctgca ttaaaattga caggaagcct cactgcatcc
    10081 tgaaatggaa cgtacatacg gaaggcatga tacgtgggtg tcagaaccat cttattccca
    10141 tcagtaaaga tcattgcctg caaaacattt atcatctggg caatgttggc gccatgcacc
    10201 cgttcggcat gccgggcgaa aatattgaaa ttcaatgctg ccacaatggc atcacgctgt
    10261 gaattctgct gctgcaggaa gttgggattc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn tgctgccaca atggcatcac gctgtgaatt ctgctgctgc aggaagttgg
    10441 gattcgtacc ttcggaaggg gcataccagg taccccattc atcaacgaga atggacactt
    10501 ttgattccgg atcgtacttg tccatgagtg ccctgttctc tgtaacaaac ttgtccatcc
    10561 tgagtgtttc ttccaatact gacgcgtact gcaactcatc aaacccggtt gccttaataa
    10621 ccggaggcca gccaccacgt gtataattat gaagcgagag tccatggata ttccatgtcc
    10681 aggttttatt tgcccaggct ttcatgatcg gttccgcata accaaaactg tactgatcat
    10741 aagagtcggg acccacagcc acatattgtg tatttacatc tgtgttatag ttagttgaaa
    10801 aggttgcgaa ttttttaagt tcagttatat actcttccgg tgtaaaagga cctccgcaac
    10861 cccacacttc gtttcccact ccccagaaag caacattgta aggttcagga tgaccgttcc
    10921 gggctctctc cgctgcaagc gctgagccgc tagccgtaag gtattcaagc cagtcggctg
    10981 attccttcac ggttcccgaa cctacattgg ctgaaataaa agcctccgaa cctatctgtt
    11041 caatgaaatc aaagaactcg tgggttccaa aagtattagg ttcaggggct cctccccagc
    11101 tgacgttaat ccttgctctc cgctcctccg gggttcctat tccatctctc cagtggtatt
    11161 tgtctgcata acagccaccc ggccatcgga catttggtac cttaagggct ttcaaggctt
    11221 caaccacatc attccttatc cctcttgtat tcgggatatc cgattcaggc cctacccaaa
    11281 tgcctccata gatgccaaaa cccaggtgtt cggcaaactg tccgaaaatg ttcctgttta
    11341 tcacaggtcc ggtttgcgag ggatcaattt taattactac tgtcgtgtca ttcccctgtt
    11401 cttttcctct ttctgagcac atggagaaaa ggaaaacaag tgcaaccatg ccctgcaaaa
    11461 cgatcagtct tttaaggtta atgttcattt acattatcag tttttattgt taatattccg
    11521 caaatcataa ttttatcaat tgcagtcccg cgctattctg ccaccaacct tgatacctgt
    11581 cgtccggcat tgagctttac cagcgtgaaa ttgaaatacc ttatagttta tcattgttcc
    11641 ctggcatact gattggtttt ccccggagtg ggtacgttac ttgccacctg tgaacctctg
    11701 tcatggttca atttaagagg agaagccagt gtaataccgc ttcctgttac cagtgcacct
    11761 gcaacgtgcg attttgtcag gggggaagtg accgtaaccg attgagccgg aacggcacct
    11821 tgaactccgg ggcgtccacg tgcagaagtt acggatgcta cgacagctgt ttcgatattt
    11881 gttccgcttc ctacagttat tgtctggcct tcgctgaacc cttcaatacc cgcaacagga
    11941 atagccgtag cccctgcatt cacatcagaa atgactgttg tgccgccgga tgtccctatt
    12001 gcctcaataa ccatactttc gatatcggac cctgaaccga tgaataccct ctgtccggcc
    12061 ttaaggtctg tgacactgcc tactttaata ttatttgatc cggctgttgc aggtagcaga
    12121 agggtggctt catcctgaag aaggaaatca tcacaattgc tgtcagtatc atttccgtca
    12181 gggaaacggc ccaagctacg attgaccatt gatgttgacc gaagtgccat accctgaccg
    12241 gaaaatcctg aacccggaac aactaccatg caaccgccct ggctctggtc accctcgaga
    12301 gttgccaatt caggacttgg tattgtgccg tttgcgctgg agttgctctg ctgtgatcca
    12361 tagaccattg cgtcgacaac aaggtttccg cttccatcca tcagggctat tgaccctgat
    12421 gccggcgaaa gaggcattcc gtacagctga tctggcttaa caggtccctg ataacccgct
    12481 gatgcgataa gaggattatt taccggggcg cccgcttcgt gatttttctc aagtgcactt
    12541 tcaagggtta taccgct
//
LOCUS       DAOPXU010000079        12512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_5630_length_12512_cov_11.884643, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000079 DAOPXU010000000
VERSION     DAOPXU010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12512
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5630_length_12512_cov_11.884643"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PKK03_08680"
     CDS             complement(<1..898)
                     /locus_tag="PKK03_08680"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNX84515.1"
                     /translation="MSARETLIAKTNRVRKAVLPGFDGAPLDLVVKLIVKGLFGRGVL
                     VTRASSIAFNMLMALLPASIFLFTLIPFIPVPNFQAELVKLFENFLPVAAYDLLETTI
                     IDVITNRSGTLLIVMFIATIIFSTNGIHALMHAFNVSMHDFASRSWIQQRKIAVFLLV
                     FILIMFSAAGGLIILSRLVVNRLVDIGVLEMNLVFYIVMAFKWLLIIVMLFFAISTLY
                     YLVPARRKDFRYISPGSILATSLFIITSLAFSAYVNNFGQYNKLYGSIGTLIVILIWL
                     YLNSVALLVGFELNVSIRAATCN"
     gene            complement(908..1750)
                     /gene="nadC"
                     /locus_tag="PKK03_08685"
     CDS             complement(908..1750)
                     /gene="nadC"
                     /locus_tag="PKK03_08685"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNX84516.1"
                     /translation="MSDNQLRSFIINAFAEDTGDGDHSSLASIPDTAAGRARLIVKES
                     GIISGLRVAREVFSIADPSLQAEILLRDGDRVQYGDIALTVSGKAQSILRSERLVLNI
                     MQRMSGIATATALYTEKVSDTQAKITDTRKTTPGMRALEKEAVRTGGGVNHRMGLYDM
                     IMLKDNHIDYAGGITAAIRRTQEYLSAHGLNLRIEVEARNIREVTEILETGGVDRIML
                     DNFSIPDTRKAVALIAGRTETESSGGITLENVRDYALCGVDYISIGALTHHIKSLDMS
                     LKAY"
     gene            complement(1743..2144)
                     /locus_tag="PKK03_08690"
     CDS             complement(1743..2144)
                     /locus_tag="PKK03_08690"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HNX84517.1"
                     /translation="MRKKIMATAGVLLTFAFLLAAQDQPRIPAGIAIAFRTGSASGLS
                     EYLNKTVELVLPGKEDFYSKSVAETILKDFFTRYRSREFVIKHQGARGDAQYAIANLE
                     TDKGKFRVYFLLKKVDGNPLIHQIRIEADNE"
     gene            2295..2768
                     /locus_tag="PKK03_08695"
     CDS             2295..2768
                     /locus_tag="PKK03_08695"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNX84518.1"
                     /translation="MKMVFLLTGKTDQSWIRDGVAEYEKRISKYGRLETITLPDVRNP
                     GNRPAEWVKAREADKMLAQLRNDDHVVLLDERGKGYSTLELADWMRRTVMLPVKRIVF
                     VVGGPYGFDTSVTARADHMLSLSRLTFSHQVVRLLFAEQLYRVLSVIAGDPYHHE"
     gene            2773..6462
                     /locus_tag="PKK03_08700"
     CDS             2773..6462
                     /locus_tag="PKK03_08700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX84519.1"
                     /translation="MAEKLKNIRIVGVALLIAVALAAVADFLYFSDLEWRYRTSCVDA
                     RLTSLENDAEKLLRTAEKQISDAGDPSSLFHSTLGSNALKKGITLLVYREGRIVYWSD
                     NSIAFPVIYEQHFDEHKPVFYSNGWFVPVHLENSEYEMLALIKVYRQYPIVNNILQTG
                     FPETFSLPDEVRITFDPSASGFRIWGSEEEFHFGLLFPETKTNTIFLAIPALLWLIVL
                     FLLVRLVILVALYIDRKSRASSGVAIAFIALLAAYGVLLLTGLPRSVTSTELFSPFNW
                     SAGRLMPTVGHVLLLGILILSGIRFTFRSGRFSKPWTGQGIFRYLVPGVMLATGFVIF
                     LLVEAYFRDLVVNSAISFEAYRILDMTFMSLAGIVSVMLLLTIPVILIMRTFRMMNGW
                     PLREILAVAGGTVLLLPLACLSGTGCNGWSILWVLVMVTTIFIWQRKSYSEVSLAIIF
                     SVITALLSTALIIKYSIIREERNMKVMAISLASDNDMVAEGMIIDMWTALEGDTVLAR
                     MVKKEIITPGDINSVYRYLEDGYFYGYWENYDISIVICRNDSPLSLGSTGTEVANCFD
                     WFDNRIRNEGDTITNTGFYFMRNRTGRAWYMSKLLYELSPGVTNGIFIELVSHIETFL
                     AGYPELLLDGGHQRFPMLKDISYAKYNKGALVLRSGEYPYDASIAGGQSEREEFRFED
                     TRDYRHLYYTLGDMTLLVTAARVKLLDRIITFAYLFIATLIYVLILLVIFTLRPAELL
                     KTDTFRRRLQLSFAAVLTVVFIIVITGALMLSTRQFRSNHTRIIIEKATSLSIELEHK
                     LSSEKTLEGGWQTPDYPTLNHLLVKFSNVFFTDINLYSPSGRLIATSRPEVFSRKLEG
                     SMIDPVAFGMLTSGGRDEYIGIESIGSMSFLSAYLSFYNEDNELLAYLNIPYFAMQNL
                     LAKETSNLIVTLVNFALLFLLIMMWVAVFLSERITRPLSMLQEAMASVAYGRKNEHIS
                     YNSRDEVGELVRQYNRMTDELEESAGKLARTEREMAWREMARQIAHEIKNPLTPMKLN
                     VQQLLKSWNDRVPDFEKRIESFTVNQIEFIDNLSNIASAFSSFAKMPVAEPAEVDVLA
                     QLNTTLAMFGHADDTTITLESGKITRAVVMADREHLNSVFSNLLKNAIQSIPPGRKGE
                     IRIVLSADENHVRVRFIDNGTGISDELIPKMFTPNFTTKSSGMGLGLSIVKRHVEGAG
                     GKIWFTTEQDKGTVFTVEFPLLFAV"
     gene            6544..10149
                     /locus_tag="PKK03_08705"
     CDS             6544..10149
                     /locus_tag="PKK03_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNX84520.1"
                     /translation="MPLSFRNIARSYLLIIVLMITFGSVLVSGQTPLLTGQVNRYAKV
                     TAVGAGFVIVDDAGDFAVSDTVMVMQMNGVEINAGTALEGNYQNTIGTPGKYEIIIVS
                     SVNTGTRRIDFTRVLMNAYDPAASVQLVKVRTYRDAVVNTQLSCQAWDSASVTGGVLV
                     FMVKGVLTLNADINVSGSGFRGGIVSQGIGWCQNTYDSLRWESYSIWSPVAGYKGEGL
                     GIRTALGQPLYPYFMKGKGANMTGGGGGNGHFSGGGGGSSYGSGSVGDQEMAPDTCGF
                     LRPGGRPGFTVASYPTLNTGVYMGGGGGASVYLTTPTTSAGANGGGIVIIHADTIVGN
                     GRIITASGAAANNNSAADAGAGGGGAGGSVIISTRHFLSAPVLSADGGKGGNNVNQNG
                     AGGGGGGGLIWTAGVFPGTATVAGGQGGIHSSGDPNRDGLPGLVRSNLNLPLNGFLFN
                     EIYIAYNQHQLDSICEGMVPPKITGTRPAGGSGTYTYQWQRSYDNNNWSTVTGSSIEY
                     TPTGTESATVWFRRVVSDGAGIIDRSLPVKIIVHPRITGNIIGSDTTLCYNMDPEELY
                     PLNSGPAGGNGLYFYQWEQSADNVTWGSAAGIGTAATYNPQALTATMHYQRRVSSGAC
                     FDISSPVTVTILPVITNNSVTADQTICEGTLFSNLSGSTPAGGAAPSFTYQWMSSTNN
                     TDWAPAPGAATASGYDVTNDSPGTTWYRRVVYSGPLNTCQSTSNSVTLICHPAITNNT
                     ISADQTICEGTAPATIDGTVPASGAGTYGYQWMSSPTGVSFSNISGATTEDLPGTTLT
                     AGIWYRRVVTSSACTSTSNNVHITVDPRITGFDISLPAQGHDTICTGTAPGLLSGTPA
                     GGLGTFTYAWASSADNSNFTPLAETGHSYQHGNLTATTWFRRTVTSGACTESSVFRIT
                     VLPQITGNVITADQTVCNTHIPAALTGGTLSGGDGKFRYLWEGKESQSPGWTAAPGTN
                     NAATYQPAMLDETTQFRRSVFSGENNCCVSVSDPVTVNVDIMPVNINAGADRTLLPYQ
                     FAAVLQGSFDGEGTSAWSYDFSSGNAEPVFTTPLEKITEVRKLEFGDNTFIFAVANGA
                     CIAPEMTVTLTVPDLTIPQGVTPNNDGINDYFNVEGLEFTRNELVIINTAGAVVFRAA
                     DYRSNDPIGAWTGLDLNGREVPDGTYYYLLTIKGAQDLDVPEYSAHISGFIILRR"
     gene            10151..11080
                     /locus_tag="PKK03_08710"
     CDS             10151..11080
                     /locus_tag="PKK03_08710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNX84521.1"
                     /translation="MKRGLHISVLLALVLALPVDGQQVPVTSQYLTNGLVINPAYAGT
                     REALSANLSYRKQWARISGAPQFQTLSLHSPVNKKEKVSLGLMADYLTYGVTKDVGIY
                     AFYAYSVRLGHGRLSMGLKGGFDLSNTNYNRLQFPDGNPADPLLTGDMKYTLPNMGVG
                     FYYYTDKYFAGLSVPSLLTYKRDEADQFRVSPDYSLFRTYLTAGTLIRFADVFKVKPS
                     VLVRYSMHEPLEVDLNANLIFADMLWVGGSYRISEKAAVALLDLQVTPQLKVGYSFDY
                     QLGHLNNYTSGTHEVSLRYEFAFAVSATSPRYF"
     gene            11085..>12512
                     /locus_tag="PKK03_08715"
     CDS             11085..>12512
                     /locus_tag="PKK03_08715"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX84522.1"
                     /translation="MRRILKHIAAAGLFLFVSIAIMSGQAKGTFEVMHISKISSSSDE
                     MAPVIMSDGILFCSNRKTNPFLTKKNLEGVRLYELYFAPFGEEGEPASPVRFAPGLVR
                     DANIGPASVATDGNTLWFTRNTSDGRRLGKKQPNRLGIFTARRSGSQWTDITPFEYNN
                     PEYNLSFPYVSADNRYLFFCSDMPGGLGRYDIYMCENIDGRWTKPENLGPEVNSSDAE
                     IHPFLHSSGRLYFASDRPGGKGGLDIWFSNLVFGSWTKPVAMDEPVNSTADDFAFYAA
                     QGGMQGYFASNRRNFNDDIYSFTSTIIRWSTCDSLQKNSYCYEFIEENALKQDTLKKG
                     FRWEWDFGDGSRASGITAEHCFSGPGIYDISLDIVNLITGGIEKRQASYELEITDIEQ
                     PVITGPDTIAAGETLILNAGDTYLPGMKIDRYYWNFGDETVATGLEVSKVFALPGRYN
                     IQLIVSSAPDAGGVVREVCVCKDIEV"
BASE COUNT         3038 a         3156 c         3319 g         2999 t
ORIGIN      
        1 tgttgcaggt ggcagccctg atactgacat tcagctcaaa gcccacaagc agagccactg
       61 aattcagata cagccagata aggataacga taagtgtccc gatggacccg tacaacttgt
      121 tgtactggcc gaagttattg acataggcag agaacgccag tgaggtaatg ataaacaggc
      181 tggtggcgag gattgacccc ggggagatat accggaagtc ctttctgcgg gcagggacaa
      241 gataatagag agtggagatg gcaaaaaaca gcatcacaat tataagaagc cacttgaagg
      301 ccattacgat atagaacacc aggttcatct ccagcacccc gatatcaacg agcctgttaa
      361 ccaccaggcg gctaaggatt atcaggccgc ctgcagcaga aaacataata aggataaaaa
      421 cgagcaggaa gactgcaatc ttcctctgct gtatccatga ccggcttgca aagtcatgca
      481 tcgaaacgtt gaaggcgtgc atcagcgcgt gaatgccatt ggttgagaat atgatggttg
      541 cgatgaacat cactataagc agggtgccgc ttctgtttgt gatgacatca ataatggtcg
      601 tttcgagaag gtcatatgct gccacaggga ggaagttctc aaacagcttt acaagttcag
      661 cctggaagtt tggaacagga atgaacggta tgagtgtgaa gaggaagatt gatgcgggga
      721 gcagtgccat cagcatgttg aaggcgatgg atgaggctct ggttacgagc actccgcgtc
      781 cgaacagtcc cttgacaata agtttcacta ccaggtcaag cggtgcgccg tcaaagccgg
      841 gcagaacagc tttccttact ctgttggttt ttgcgatcag cgtttccctg gcgctcatac
      901 aaggttatca ataggctttc aggctcatgt caaggctttt tatatggtgg gtcagagccc
      961 ctattgaaat gtaatccacg ccgcagaggg cataatccct cacgttttcc agggtgatgc
     1021 ctcccgatga ttcggtctca gttctcccgg cgatcagagc cacggctttc ctggtatcag
     1081 gaatactgaa attgtcaagc atgatccggt caacgcctcc cgtttcaagt atttccgtaa
     1141 cctccctgat gttccgtgct tccacttcga tcctcaggtt gagtccgtgt gctgacaaat
     1201 actcctgtgt ccgcctgatt gctgcggtga tacccccggc atagtcgata tggttatcct
     1261 tcagcatgat catatcatag aggcccattc ggtggttgac tcctcctccg gtacgtactg
     1321 cctccttttc gagggcgcgc atacccgggg ttgttttgcg ggtgtcagtg atttttgctt
     1381 gtgtgtcact gactttttcg gtatagaggg cggtggcggt ggctatgcca ctcattctct
     1441 gcataatgtt aagcaccagt ctctctgagc gcaggataga ctgagccttg cctgacacgg
     1501 taagagctat gtcgccgtat tgaaccctgt caccgtcccg aaggagtatc tctgcctgca
     1561 gtgacggatc tgcaatgctg aaaacttcgc gtgcaacgcg aagcccggag atgatgccgc
     1621 tttcctttac tataagcctg gcacggcctg cggcggtgtc agggatggat gcaagtgagc
     1681 tgtgatcgcc gtcgccggtg tcttcggcga aggcattgat tataaaagac cgcagctggt
     1741 tatcactcat tgtcagcctc gatcctaatc tggtgtatca gggggttgcc gtcaactttc
     1801 ttcagcagga aataaacccg gaacttgccc ttatcggttt ccaggttcgc tattgcatac
     1861 tgggcgtcgc ccctggcccc ctggtgcttg atgacaaatt cgcgtgagcg gtagcgggtg
     1921 aagaaatcct tcagtattgt ctcggctaca ctcttactgt aaaaatcctc cttccccggc
     1981 agaaccagct caactgtctt gttgaggtat tcggagagcc ccgaagcgct tcctgtacgg
     2041 aaggcgattg cgatacctgc aggtatcctg ggctgatcct gggcagcaag cagaaatgca
     2101 aaggtgagaa gcacacctgc cgttgccata atcttctttc tcattttgtt tcctttatgc
     2161 tttacacgta aggacaaagc aaatttaacc aaatatatca aaaaccgaac cacttttaat
     2221 tatcaggaag acgcacccgc aataaagggc ttaattgcta tatttgccat actcaacaga
     2281 aaccggggag agtaatgaag atggtgttcc tgctgactgg aaaaactgat cagtcatgga
     2341 taagggacgg ggtggcggaa tatgagaaga ggatctcaaa gtacggccgt ctcgaaacaa
     2401 taacccttcc cgatgtccgg aacccgggta accgcccggc agaatgggta aaggccaggg
     2461 aggcagataa gatgctggcg caactccgga atgatgatca cgtggtcttg cttgatgaac
     2521 gcgggaaggg ttattcaacg ctggaacttg ctgactggat gcgccggacg gtgatgctgc
     2581 cggtaaaaag gattgtgttc gtggttggag ggccctatgg gttcgatacc tcggtaactg
     2641 caagggcaga tcatatgctc tcgctttcaa ggctcacgtt ctcacaccag gtggtacgat
     2701 tattgttcgc ggaacaacta tacagggtac tttcggtcat tgccggggat ccctatcatc
     2761 atgaatagcc ttatggctga gaaactgaag aacatcagga tagtaggagt cgcccttctc
     2821 atcgctgttg ccctggccgc agttgctgac tttctctatt tcagcgacct ggagtggcga
     2881 taccgtacat catgcgttga cgccaggcta acatcacttg agaatgatgc tgaaaaactg
     2941 ctcaggacag ctgaaaagca gatctctgat gcgggtgatc cttcatccct gttccacagt
     3001 actctcggca gcaacgccct taagaagggg atcacactgc tggtttaccg tgagggacgc
     3061 atagtctact ggtcagacaa cagcattgcc ttccccgtga tctatgagca gcattttgat
     3121 gagcataagc cggtcttcta cagcaacggc tggttcgtac cggtgcacct ggaaaacagt
     3181 gaatatgaga tgctggcact gatcaaggtg tacaggcagt acccgattgt gaacaatatt
     3241 cttcagacag gctttcccga gacgttttct cttcctgatg aggtccgtat cacctttgac
     3301 ccttccgcct cgggcttcag gatatgggga tcggaggagg aatttcactt cggccttctt
     3361 ttccccgaga cgaagaccaa caccatattc ctggccattc cggcactgct ctggcttata
     3421 gtccttttcc tgctggtaag gctggtgatc cttgttgccc tttacattga caggaaatcc
     3481 agggcgtcgt caggtgttgc cattgccttt attgcgcttc tggcggcata cggagtcctt
     3541 cttctgacgg gattgccgcg ttcggttact tcgacagagc tgttttcacc gttcaactgg
     3601 tcagccggcc ggctgatgcc gacggtaggc cacgtcctgc ttctcggcat tctgatcctt
     3661 tccggtatcc ggttcacatt caggagcggc aggttcagca agccatggac ggggcaggga
     3721 atattcaggt acctggtccc gggagtaatg ctggcaacgg gatttgtcat cttcctcctg
     3781 gttgaggcct atttcaggga cctggtggtg aattcggcaa tcagttttga ggcctaccgg
     3841 attcttgaca tgaccttcat gagcctggcc ggcatagtct ctgtgatgct gttgctgacc
     3901 attcccgtga tacttatcat gcgcacattc aggatgatga acgggtggcc cctgcgtgag
     3961 atccttgcgg ttgccggcgg aaccgtgctg ctgctccctc tggcatgcct gtccggtacc
     4021 ggctgcaacg gctggagcat cctgtgggta ctggtgatgg tcaccacgat ttttatttgg
     4081 cagaggaaat cctactccga ggtatcactc gcaatcatct tttcagtaat aacggccctg
     4141 ctctccaccg cacttattat caaatactca atcatcaggg aggagagaaa catgaaggtc
     4201 atggccatct cccttgccag cgacaatgat atggtggcag aagggatgat cattgacatg
     4261 tggacggcgc tggagggaga caccgtgctt gcacggatgg tcaaaaagga aataatcact
     4321 ccgggagaca tcaattctgt ctacaggtat cttgaggatg gctacttcta cggttactgg
     4381 gaaaactatg atatcagcat tgttatctgc cgcaatgact cacccctcag cctgggttcc
     4441 acgggcacag aagtggccaa ctgcttcgac tggtttgaca accgtataag gaatgagggt
     4501 gacaccatca cgaataccgg tttttacttc atgcgcaacc gtaccggaag ggcctggtac
     4561 atgtcaaagc tcctgtatga gctatcgccg ggtgtgacca acggaatctt cattgagctg
     4621 gtcagccata tagagacgtt cctggcaggc taccctgagt tgcttctcga cggggggcat
     4681 cagagatttc caatgcttaa ggacatctca tacgcgaaat acaacaaggg ggcgctggtc
     4741 ctgaggtcag gcgaatatcc gtacgatgct tccattgccg gagggcagtc ggagagggag
     4801 gagttccggt ttgaagatac cagggattac aggcacctgt actatacact gggtgacatg
     4861 acccttttag tcaccgcagc cagggtaaag ctgcttgacc ggataatcac ttttgcctat
     4921 ctcttcattg ccacccttat ctacgtttta atattgcttg tcatcttcac tctgagacct
     4981 gccgagctcc tgaaaacgga tacatttcgc cggcgcctgc agctgtcatt tgcagccgtt
     5041 cttaccgtgg tcttcattat tgtgatcacc ggtgcgctta tgttaagcac caggcagttc
     5101 cgcagtaatc ataccaggat cataattgaa aaagcgacat cgctctcgat agagctggag
     5161 cacaagctct cctctgagaa gacgcttgag ggagggtggc agacaccaga ctatcccacg
     5221 cttaaccatc tgctggtgaa gttctcaaat gtattcttca ctgacatcaa tctctattca
     5281 ccttccggga ggctcatagc aacatcgagg cccgaggttt tttcgcgcaa gcttgaaggc
     5341 agcatgattg atcctgtagc tttcggtatg cttacatccg gaggcaggga tgagtacatt
     5401 ggtattgaga gcattggcag catgagtttc ctctcagcct atctgtcatt ctacaatgag
     5461 gacaacgagc tcctggcata tctcaacata ccctattttg ccatgcagaa cctcctggca
     5521 aaagagactt ctaacctgat tgtcaccctt gtaaactttg cactgctctt cctgctgatt
     5581 atgatgtggg tggcagtttt tttgagtgaa aggattacca ggccgctcag tatgcttcag
     5641 gaggcaatgg cttcggttgc ctacgggagg aagaacgaac atataagcta taacagtcgc
     5701 gatgaggttg gcgagcttgt gagacaatac aacaggatga ctgatgagct ggaagagagt
     5761 gccggcaagc ttgcgcgcac cgagcgcgag atggcctggc gggagatggc caggcagatt
     5821 gcccatgaga tcaagaaccc tctcaccccg atgaagctca atgtgcagca actgttaaag
     5881 tcctggaatg acagggttcc tgatttcgag aagagaatcg agagctttac agtcaaccag
     5941 attgaattta tcgataacct ttccaacatt gcctcagcct tctcatcatt cgcaaagatg
     6001 ccggtggcag aacctgccga ggtagacgtc ctggcccagc tgaacaccac tcttgccatg
     6061 ttcggccatg ccgatgatac aaccatcacc ctggaaagcg gtaaaatcac cagggcagtc
     6121 gtgatggctg acagggaaca tctgaacagc gtcttttcca acctgctgaa gaacgcgata
     6181 cagtctatac ctcccgggag aaagggagaa atcaggatag tgctttctgc tgatgaaaac
     6241 catgtaaggg taagattcat tgacaacggc accggtattt cagatgagct gatccccaaa
     6301 atgttcactc ctaattttac cacaaaatcg tccggaatgg ggcttggttt atcaatcgta
     6361 aaacgacatg ttgagggagc cggcggaaag atctggttta caacggaaca ggacaagggg
     6421 acagttttca ccgttgagtt tcccctgctc ttcgctgttt agcctcatta aacacggggc
     6481 tcctaaaata atctgcggtt ttctcagttt taataacgta gattagcaaa ccagttcctc
     6541 aagatgcccc ttagtttccg taacatagcc aggagttatc ttctgataat tgtgctgatg
     6601 ataaccttcg gttctgtatt ggtgtcaggg cagacacccc ttcttaccgg ccaggtcaac
     6661 aggtatgcga aggtcactgc cgttggtgcc ggttttgtca ttgtggatga tgccggtgac
     6721 tttgctgtca gtgataccgt tatggtgatg cagatgaacg gggtggagat caatgcgggc
     6781 acagccctgg aggggaatta tcagaacacg ataggaactc ccggcaagta tgaaataata
     6841 attgtgtcat cagtcaatac gggcactcgc aggattgatt tcacccgggt gctgatgaat
     6901 gcctatgatc ctgcagcaag tgtacagctc gttaaggtaa ggacttaccg tgatgctgtt
     6961 gtcaacaccc agctttcatg ccaggcatgg gattcagcct cagtcaccgg aggggttctg
     7021 gtgttcatgg taaagggagt tctgacactc aatgcagata taaatgtttc cgggtccgga
     7081 ttcaggggag gaatcgtatc acagggcata ggctggtgtc agaacacata tgacagtctc
     7141 aggtgggaat catacagcat atggtcacct gtcgcagggt acaaaggtga aggtcttggc
     7201 atccggactg cccttggcca gcctctttat ccttacttca tgaagggtaa gggtgcgaat
     7261 atgaccggtg gtggcggtgg caacggtcac ttctcgggcg gcggcggcgg ttccagctac
     7321 ggttccggca gtgtagggga ccaggagatg gctcccgata cctgcggttt cctcaggcca
     7381 ggcggccggc cgggattcac ggttgccagt tatcccacac tgaacacagg agtttatatg
     7441 ggtggcggcg gcggcgcttc tgtatacctg actactccca ccacctctgc cggagcaaat
     7501 ggtggcggaa ttgtgatcat tcatgctgac accattgtgg gtaacggcag aatcatcact
     7561 gccagcggcg cagcggcaaa taacaattct gctgcagatg ccggtgccgg cggtggcggt
     7621 gcaggaggct ccgtgataat aagcacaaga catttcctct cagccccggt tctctctgcc
     7681 gatggcggca agggaggcaa taacgtaaac cagaatggtg cgggtggcgg cggtggcggc
     7741 ggactgatat ggactgccgg ggtcttcccc ggaacggcaa cggttgccgg cgggcagggc
     7801 ggcatacaca gcagcggcga tcctaaccgt gacggtttac ccggccttgt acgcagtaac
     7861 cttaacctgc cgcttaacgg attcctcttc aatgagatat acatagccta caaccagcac
     7921 cagcttgact caatctgcga agggatggtt cccccgaaga ttaccggcac gaggcctgca
     7981 ggagggagcg gtacatacac ttaccagtgg cagagaagct atgacaacaa taactggtca
     8041 accgtgaccg gttcttccat cgaatacacg cccacaggaa ccgaatcagc cactgtctgg
     8101 ttcaggaggg tggtcagtga cggcgccggt atcattgacc ggagcctgcc tgtgaagata
     8161 atcgttcacc ctcgcataac cgggaacatc ataggcagtg acaccacgct atgctacaac
     8221 atggatcctg aggagctcta cccgctgaac tcaggtccgg caggaggaaa tggcctctac
     8281 ttttaccagt gggagcagag cgctgacaac gttacatggg gaagcgctgc cggcattggc
     8341 actgcagcga catataatcc tcaggccctg actgccacta tgcactatca aaggagagtc
     8401 agttcgggag cctgcttcga tataagctca cctgtcactg taacgatatt gcctgtcatc
     8461 accaataaca gtgtaacggc tgaccagacc atctgcgaag ggaccctctt cagcaatctg
     8521 tcaggcagca cccccgccgg cggagcggct ccctcattta cctaccagtg gatgagcagc
     8581 accaacaaca ctgactgggc tccggcacct ggagcagcca ctgcatccgg ctacgatgtg
     8641 acgaatgatt caccaggtac aacctggtac aggagggtgg tttacagcgg cccgcttaac
     8701 acatgccagt caaccagcaa cagtgtcacc ctgatctgcc acccggcgat aaccaacaat
     8761 accatatctg ctgaccagac aatctgtgaa ggcactgcac cggcaaccat tgacggtacc
     8821 gttcctgcca gcggagccgg cacctacggc taccagtgga tgagcagccc caccggggta
     8881 tcattcagca atatcagcgg cgctaccact gaagacctgc cgggcaccac tcttactgcg
     8941 ggtatctggt acaggcgtgt ggtgacctca tcagcctgca ccagcaccag caacaatgtt
     9001 catatcacgg ttgatccgcg tattaccgga tttgacatta gcctcccggc acaggggcat
     9061 gatacaatat gtacaggaac cgcgccgggc ctcctctcgg ggactcccgc cggcgggctg
     9121 ggcacattca catacgcatg ggcctcatct gcagacaaca gtaacttcac gccactcgct
     9181 gagacaggtc actcgtacca gcatggcaac cttacggcaa caacatggtt ccgcaggact
     9241 gtcacctctg gtgcatgcac agagagttca gttttccgga tcaccgttct gccgcagata
     9301 accggaaatg tcatcactgc tgatcagacg gtatgcaaca ctcatatccc ggcggccctc
     9361 accggcggca cactctcagg cggtgacggc aagttcaggt acctgtggga agggaaagag
     9421 tcacagtcac ctggctggac agctgcgcca ggcacgaaca atgccgccac ctatcagcct
     9481 gcgatgcttg acgaaacaac ccagttcagg aggagtgtct tctccggtga gaacaactgt
     9541 tgtgtttcag tatcagatcc ggtcacagtg aatgttgaca tcatgcccgt caatatcaat
     9601 gcgggtgctg accgcacact tcttccctac cagttcgcag ctgttctgca gggaagtttt
     9661 gacggcgagg gaacctcagc ctggagttat gatttcagca gcgggaacgc agagccggtc
     9721 tttacaacgc ccctcgaaaa gatcaccgag gtaaggaagc tggagttcgg agacaacact
     9781 tttatcttcg ccgtcgcaaa cggtgcatgc atagcgcctg agatgactgt gacacttaca
     9841 gtgcctgatc ttaccatacc ccagggggta accccaaaca atgatggcat aaatgactac
     9901 ttcaacgttg aggggctgga gtttacacgt aatgagcttg ttattatcaa cacggcaggt
     9961 gctgttgtgt ttcgggctgc ggattaccgc agcaatgatc cgattggcgc ctggacaggg
    10021 cttgacctga acggacggga ggttcccgat ggcacctatt attacctgtt gaccataaag
    10081 ggagcgcagg accttgatgt tcctgaatac tcggctcata tcagtggatt tatcattctc
    10141 aggagataag atgaagagag gtttgcacat atcagttctc cttgcattgg tgcttgcgct
    10201 gccggtggat gggcagcagg tgcctgtgac cagccagtat cttaccaacg gccttgttat
    10261 caatccggct tatgcaggta cccgcgaggc gctgagcgcc aatctctcgt accgcaagca
    10321 gtgggcacga ataagcggtg ccccgcagtt ccagacactt tcactgcaca gcccggtaaa
    10381 caagaaggaa aaagtatcac tcggactgat ggctgactat ctcacatacg gtgtgacaaa
    10441 ggatgtgggt atatatgctt tctacgctta cagcgtaagg ttaggccatg gaaggctttc
    10501 aatgggactt aagggcggct ttgacctcag caacaccaat tacaaccgtc tgcagttccc
    10561 tgacggcaac cctgctgatc ctctcctcac gggagacatg aagtacaccc ttccaaatat
    10621 gggagtcgga ttctattatt atactgacaa atacttcgcc ggattgtcag taccatctct
    10681 gctgacttat aaaagagacg aggctgacca gttcagggtg agtcctgact attccctgtt
    10741 caggacatat ctcacagcag gaacactgat aaggtttgca gatgtgttta aggtcaagcc
    10801 ttcggtactt gtgaggtatt caatgcacga gccactggaa gttgacctga atgccaacct
    10861 gatttttgcc gacatgctat gggtcggagg ttcatacaga atttcagaga aggcagcggt
    10921 tgccctcctt gacctgcagg taacacctca gcttaaggtt gggtattcct ttgattacca
    10981 gcttggtcat ctgaataatt atacatccgg gacacatgag gtgtcactga ggtatgagtt
    11041 tgcatttgcc gtaagcgcaa catcgccaag gtatttctga aaatatgagg agaattctga
    11101 aacatattgc agcagcgggg cttttcctct tcgtcagtat cgctatcatg agcggacagg
    11161 cgaagggaac ctttgaggtc atgcatatct caaagatcag ctcctcttcc gatgagatgg
    11221 ctccggtgat aatgagtgac ggaatcctgt tctgttcgaa caggaagacc aatcctttcc
    11281 ttacgaagaa aaaccttgag ggagtcaggc tttatgagct ttattttgcc cctttcggtg
    11341 aggagggtga gcctgcctca cctgtccggt tcgcaccagg tcttgtcagg gatgcaaaca
    11401 ttggcccggc ctctgtggca actgacggaa acaccctgtg gttcacgcgc aataccagcg
    11461 atggccgccg gctcgggaag aaacagccca acagactcgg catctttaca gccagacgtt
    11521 ccgggtcaca atggactgat atcaccccct ttgaatacaa taaccctgag tacaatctgt
    11581 ccttcccata tgtttcagca gacaacaggt atctgttttt ctgttctgac atgccgggag
    11641 gactgggcag gtatgatatc tatatgtgcg agaatattga cggacgctgg accaaaccgg
    11701 aaaatcttgg tccggaggtc aacagcagcg atgcagagat acatccgttc cttcacagtt
    11761 ccggcaggct ctatttcgct tccgacaggc ccgggggaaa gggggggctt gatatctggt
    11821 tttcaaacct ggttttcggc tcatggacaa agcctgtggc aatggatgaa ccggtgaact
    11881 cgacagcaga cgactttgcc ttttatgctg cgcagggcgg catgcagggc tattttgcct
    11941 caaaccgccg caatttcaat gatgatattt acagtttcac atcaaccatt atcagatggt
    12001 caacctgcga ttcacttcag aaaaacagct actgttacga gttcatagag gagaacgcgc
    12061 ttaaacagga taccctcaag aaaggtttca ggtgggagtg ggacttcggt gacgggtcaa
    12121 gagcctcagg cataactgcg gaacactgtt tcagcggtcc gggaatatat gatatcagcc
    12181 ttgatattgt gaaccttatt accgggggca ttgagaaacg acaggcatcc tatgagcttg
    12241 aaattacaga tatcgagcag ccggttatca ccggcccgga tactatcgca gccggggaaa
    12301 cgttgatcct taatgcaggt gacacttacc tgcccgggat gaagatagac cgctactact
    12361 ggaatttcgg agatgagact gtagcaaccg gactggaagt atcgaaggta tttgcccttc
    12421 cgggaagata taatattcag ctgatagtca gttcagcacc tgacgccggg ggagttgtgc
    12481 gtgaggtctg tgtctgcaag gacattgaag ta
//
LOCUS       DAOPXU010000080        12173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_5848_length_12173_cov_13.375722, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000080 DAOPXU010000000
VERSION     DAOPXU010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12173
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5848_length_12173_cov_13.375722"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="PKK03_08720"
     CDS             complement(<1..1371)
                     /locus_tag="PKK03_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNX84523.1"
                     /translation="MESDKINRILEGQKRFFAEGKTLDVRYRLEVLKKLRKLIVKNEK
                     EIMNALRQDFHKPEFESLATETRFVIKEINLAIRRLRKWTGKKHVWTPFVHFLAFSYI
                     TPQPYGQVLVLSPWNYPFQLSFMPLVGALAAGNCVVMKVSRQVKAVTKVMEKILDEFP
                     KELIVMMNGDHTVSEYLLSYKFDYIFFTGSCETGKYVMQKAAENMIPLSLELGGKNPC
                     VIASDAKLNYAAKRIAWGKFMNAGQTCICSDYVLIDRVVKDRFLELITNELQRFYGSD
                     PLKSSDFARIINKDNVRRLSALMQAGEIVTGGITDEENCYVSPTVIKDIKPDDPIMKE
                     EVFGPLLPVIEFERFEEVYSIIEMNPKPLATYIFTDNKKLAREFLNRTQSGTAGINDT
                     VIQIASPYLPYGGVGCSGIGRYHGRKSFETFSNMRSVIRKSNLFDLSLRYPPYNKFKE
                     NIIRLLM"
     gene            complement(1353..2975)
                     /locus_tag="PKK03_08725"
     CDS             complement(1353..2975)
                     /locus_tag="PKK03_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNX84524.1"
                     /translation="MIYDYIVIGSGFGGSVASLRLAEKGYKVLVLEQGKRYDKDDFPR
                     TNWNLPRYLWLPPLRFFGFQKLSFYSKASILSGTGVGGGSLVYANTLYVPPDEFFQNP
                     AWSRFGNWKEILSPFYNKASFMLGRQKYPKLNVEDKVLEEVAKEMNAHSSFETVYVGV
                     NLGEKSDPVDPYFNGLGPLRNKCTECGGCMVGCRENAKNTLDKNYLWFAEKLGVEILP
                     ETRAEKIIIQDDLYHVHTRKVTSLFGTKANTFTSKGIVVAAGTLGTLELLLKQKYKYG
                     TLPLLSEKLGHELRTNAETLCAVSGADRKLNNGLAITSVFSPDPHTHVEIVKYPDNSN
                     AMKFFFGLSADGAATSLKRSWNLIVNTLKHPVQFLKTVLNFRWSSNLVILLVMQTVDN
                     AMKMVWKKTAFGGRMVIDNSGNKKVPAYIPVGQEVMLRYADKVKGIPQNILLEVLFNR
                     PTTAHILGGCPMSESAESGVVDNSFRVHNYPNFYITDGSVVQGNIGVNPSLTITAIAE
                     YAMNRIEPKDNCHIQDISQKLKLLEEEWNQTK"
     gene            complement(3533..5524)
                     /locus_tag="PKK03_08730"
     CDS             complement(3533..5524)
                     /locus_tag="PKK03_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84525.1"
                     /translation="MKTKRITVIAFALLVFMQIAVAQNYKSQSYATGNYIFCGKELAK
                     NFSWLIEKQDAKGKWISVAEIQAPKNAADCRARLMSLPQVVSAVTEISDGMTDFVWNK
                     IKSPSATLDSLYIHSTDPRWQYVAGAGWFDNETKTPGTYKYRISRLSGSRSKTQGREI
                     SVSFPPEPSADEAIPVRYKVNMGYIEISYELSDIGNTAGLKLQRSPYMQNAFSEISAE
                     TMFAQEKGKMVALVRDRNVTTGMIYSYVAIPYDGLGNLGKQSEPVNVNFITKPADIGI
                     VTEFTVTPQPEKGGNLLKWDYDHAAFVNAIEIYRSTSYNDNYKRVVSLPAGQNEYFDG
                     SDLQPSITYYYYAVLNNGTGNSLPSARVPVILRGNKENIIPPQDLTATRSGNVVTLRF
                     RRVGYDIHGYYVYRADGYTSELHQLERMLHSTDSLLTYTDTLPVSSRSAVYSYAVASV
                     NTSYNISPVSSRVSISFSGGLMPVPDRLNALLEENAVKLVWSDVAGLNPSVTAYEVYR
                     STRSQGKEYGNEQLLGTVNFMSNSFTDRQVMPGRNYVYRVRCIAADTTDVSSFSLPYS
                     IYVPAGSALPPGEVSAIASSKVITLKWTLPVAGDIQSVLVYRAPENGKESLLKTLDGK
                     AESFTDDTAEKGKMYYYFIVVKYKNGAESRPTDAVSARI"
     gene            complement(5536..12051)
                     /locus_tag="PKK03_08735"
     CDS             complement(5536..12051)
                     /locus_tag="PKK03_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84526.1"
                     /translation="MKYSFKGFPELYPVTGTDVIAEALVPIYPGNTDPAPVITVKNRS
                     YKKVASVALTTDDQGKINSQLPVPVNYIVNDTIYCRLLIGNQYYVDDEFDLKIVTHQD
                     HTVSQDPSSQNGTPGSGEFLTWAEGRSSFADFGELVAKTYAYRLNLKVQKRFTSYFVS
                     QDGTNVTVQHKEKTEAQKALEKMKTQGVENTLNDTQQAGYYESEIVAVAPGIPVILYR
                     ENKKPHIPAWEGNIDMKTPLTDGKITVIAKGITEIQGDKSYAVFDRLLASNSEKYKII
                     AVKDIDNWVSKQNQNINISGAVKTGAVKTDAVKTGAVNATQTNADAAKTAYKFNPATP
                     ISASEKMTLEAAIESIITAGTSTTNFLDEDDYIAEPMEFSLALPETGDPDAYYRTVSA
                     DYDVVSCRPPTSVIRGRLLYTWKSDKGKLKSPLANTRFRVIVNYVDENNVSIGATKSQ
                     YNNIPGLPGGSESYSFSPDNSGGDTDEWFSLSDQYATMAAGITDANGNFVIETVNLDF
                     KGDLGPGTVTHSKTSHSVPETTPGGGIGDQFKKELGAGLQGMDQYVTNPWNDGSSFAF
                     PSGPSIAGLKPGGNGFGTGNASLSGQSSINPGFNVSFDEGLNSFELYGSKLKGDKSIT
                     TGGEMFFPEPDAPAHGPNPSVNSLPATNPCPIPAYAAEDEYETKPYKGFKRVFRIIID
                     GDAASYYYPSAEIITVQPLEEIQTPIDITHYVREFEMKVYPKTLNEDKEKISLSGMQV
                     TLFRNLADKKKNLPHGEGDGKYTYAELLNPQYNTQDAASTSDKIDAGGIFSKKFEQLW
                     PARPTVYNSASQSNYSSLPALLQSQYPYYFIESSSYVAENNLTYQATIRESPQVPQVT
                     ETNWANPEIPVVEADVILQPLVSRALITVRDVKSGSVVTKNHGARVMLSKEQSISGNS
                     NFKTVPVDDYGKAEILASGGSALDEYVADGQNPTKVYFFAGANGYRDSGAGAGYSFIR
                     KGVQFVKDIGLSPAHTVTGTVRCPDEKLPDNTLATVQAYVQVNDGKYFETYGSGYFEI
                     PAPYMAKDTLKIIPKDVAYFNSSYVFTDADAAKDLLKLNDVLVFRRRHRIQFSVTEKL
                     DQAGPPKSIAGATIQLGDSVRTTDAGGIARFNFENVSVNNYTFVVRGAAGSGYIPKTV
                     NVNSKETKDFVIKSVQLEKGSEISGLVKLDGTPVKNAKVYIDVSNTSTQVASSLNISQ
                     KSGSADYPVVNLNGPGFNKPEGKITQDANLVAAYTDSQGKYTLRGVPVDNQEIFVRAT
                     LDTTFTVAGDKQPASIKGGKAVADLNLKSYSQAVINKIYGFPLTVESLTPVNQKQVKV
                     TGLIHWTEAISDFSLNEVNKLLRIEDVVFNLVSKNGSVVGIANDDEVVIKGVTMLKLS
                     YLDSYNIALQSAGTQNIFNTQPLTVKRVNDFGKIDGKMKIVDNSFNYPSSYLNFDKSE
                     FFLAKKQNSAISNIVELVTSAVSETEASKPAYDNLSVYQKAIQTGYIDYLSKPKPVYY
                     LCDRNAAPIAFKLLNFPATANPLNSFIDDNGKIHLDVDLTCSIANAKPENFTVNIPGI
                     VLDQNKVSPASGTVPIKLALEDWTLEVKDWKLDPSEGGIVSQNAMIRTKIVDIPLTRF
                     VLRNDMFLIDKFQFDKIALAGGIFPLKDVDQTKAHLNFEQKVGSDMNPHWNFNLISNG
                     TEKVASLPGLAGLTNPDLVTPYPVDFDYLQILSNNEMIVQLKQSGVKARLKGNSLASF
                     VPQSIYNGPNYMKLSGTLNVGAPRVSDILLNAIWKSPTANPDFENVSTDFETKGFVHF
                     YSSLKPIAIEKNEIRISGKVIEKPSKTFNPIPSTFYARVTGTPHYEVKMQKDWVTQLT
                     SEEDESITSPVASSKGYSLKIAEGGMSVTGNDWTTCKFTGDLTNNDKSKTDDIAPTKT
                     TFEVLGDISASSDKLSVSAINTPFGSMTQTFDFKTKELIGTLSFNYELTLGSVVIHKG
                     TISSCFGAEGFYIIGACNAFLPIGILAGNYNTGLMLGSHKLTDEMWQLTNIYIDDRVV
                     NWCYKKNTERLSGFYFAFNRELFKAREDFDYVLAKGYVDALGLIGGNTYVNVTGGSWQ
                     VGLGGYVYLHAGAGLSAITGTSISGSVGGDGRIEFQLGDPTYIKTSINLGFSAKISQD
                     LGVKTVSKSIDVNASAEGGSGGFRFLLESGTDKLSSCKE"
BASE COUNT         2935 a         2889 c         2776 g         3573 t
ORIGIN      
        1 cattaacaac cttataatgt tctctttgaa tttattatat ggcggatagc gaagactcag
       61 atcgaaaaga tttgattttc ttataacgga gcgcatattg gagaatgtct cgaatgattt
      121 ccttccgtga tatcttccga ttccgctgca tccaactcct ccatatggca gatacggcga
      181 tgcgatctga atgacagtat catttatccc ggccgtaccg ctttgggtcc tgttcaggaa
      241 ttcgcgtgcc agctttttat tatctgtgaa aatgtatgta gcgagaggtt ttggattcat
      301 ttcaataatg ctgtagactt cttcgaacct ttcaaattca ataaccggca gcaatggacc
      361 aaatacttct tccttcatga tcggatcgtc cggtttaata tcctttataa cagtcggaga
      421 aacataacag ttctcttcat cggttatccc cccggtaact atttcacctg cttgcatcag
      481 tgcggataat cttcttacat tatccttgtt gattattctg gcgaaatcac tgcttttcag
      541 gggatcagag ccgtagaacc tttgtaattc atttgttatc aattcaagaa acctgtcttt
      601 cactactctg tcaatcaata cataatcgga acagatgcaa gtctggccgg cattcatgaa
      661 tttaccccag gcaatccttt ttgctgcata gttcagtttt gcatctgaag ctataacaca
      721 gggatttttg cctccgagtt caagtgacag gggaatcata ttttcagcag ccttctgcat
      781 aacatattta cctgtttcgc agctgcctgt gaaaaaaata tagtcgaact tgtaactgag
      841 caggtattcg cttacagtat gatctccgtt catcataact ataagctctt ttggaaattc
      901 atcaaggatt ttttccatca ctttggtgac agcttttacc tgccttgata ccttcattac
      961 gacacagtta ccagccgcga gggcaccaac aagaggcatg aatgaaagct ggaacgggta
     1021 gttccatggc gagagaacga gcacctgacc gtatggctgg ggagttatat agctgaaagc
     1081 aagaaagtgc acaaacgggg tccagacatg ctttttcccg gtccattttc taagtctgcg
     1141 aatcgcaaga tttatctcct tgatcacgaa ccttgtttcg gttgccagag attcaaattc
     1201 aggcttatga aaatcctgcc gcaaagcatt cattatttcc ttttcattct ttacgataag
     1261 cttcctgagt tttttcagaa cttcaagcct gtaacggaca tccagggttt tgccttcagc
     1321 aaaaaatctc ttctggcctt ccagaatcct atttattttg tctgattcca ttcctcttcc
     1381 aggagtttta gtttttggga tatatcctga atgtgacagt tgtcctttgg ctcgatcctg
     1441 ttcattgcat attctgctat tgcagtgatg gtcaggctcg gatttactcc gatgtttccc
     1501 tgaacgacag atccgtcagt gatatagaaa ttggggtaat tgtgaaccct gaagctgttg
     1561 tcgaccaccc ccgattcagc tgattccgac atcgggcacc ctccaagtat gtgggcggta
     1621 gttggtctgt taaataacac ttcaagaagg atgttctggg ggatgccttt tactttatcc
     1681 gcataccgta gcattacttc ctgcccgaca ggaatatatg caggtacctt cttgttaccg
     1741 ctgttatcaa taaccattct gccgccgaac gcagtcttct tccagaccat cttcatggca
     1801 ttatccaccg tctgcataac aagaagtata acaaggttgg aggaccacct gaagttcagg
     1861 acggttttaa ggaattgcac cggatgcttt aacgtattaa ctatgagatt ccatgatctt
     1921 ttaagcgaag ttgccgcccc gtccgccgat aaaccaaaga aaaatttcat tgcatttgag
     1981 ttatcgggat actttacgat ctcgacgtga gtatgagggt caggactgaa cactgaagtt
     2041 atggcaagcc cgttattaag tttccggtcc gccccggaga cagcacacag tgtctcggca
     2101 tttgtccgga gctcatgacc aagcttttcg gataacagag gcaatgtacc gtacttatat
     2161 ttctgcttaa gtaacagttc aagcgttccc agtgtgcctg ccgcaaccac aatacctttt
     2221 gaggtgaagg tatttgcttt tgtcccgaac agggaagtaa cttttctggt atgaacatga
     2281 tacagatcgt cctgtatgat aatcttttct gcccttgttt caggaagtat ttcaacacca
     2341 agcttttcag cgaaccagag gtagttttta tcgagagtgt ttttggcgtt ttccctgcat
     2401 ccgaccatgc atccgccgca ctcggtgcat ttatttctca gaggtcccag tccattgaaa
     2461 tatggatcaa ccggatcact tttttccccg agattaactc cgacatagac agtctcaaat
     2521 gaactgtggg cattcatttc ttttgcgact tcctcaagaa ctttatcctc gacgttcaat
     2581 tttgggtatt tctgccttcc gagcatgaag gaagctttat tgtagaaagg gctgagaatt
     2641 tctttccagt ttccgaagcg ggaccatgcc ggattctgga agaattcatc gggagggacg
     2701 taaagggtat ttgcatagac aagacttcca ccaccgacgc cggttccgct cagtatgctt
     2761 gccttactgt agaaggagag cttttgaaat ccgaaaaacc ttaaaggggg gagccacagg
     2821 taccttggaa ggttccagtt ggtacgaggg aaatcatctt tatcatagcg tttgccctgc
     2881 tcaagaacca gtaccttata acctttttca gcaagtctca ggctggcgac acttcctccg
     2941 aaacctgatc ctataacgat atagtcatat atcattatta aaaatggatt ggttaaaaac
     3001 aacagagcgc taatatagaa agacctgcag aaaatcagca ggaagcagat gaaaaatcaa
     3061 agagcgggac catcaggaat cccgttagac aagagtcaga ctgcaaaaac cagaggcctc
     3121 ctcaacattc agccaggttt ctcaccactt tgtgccatgg cactcaaaac aggatacccc
     3181 tgtatttccc ccgcgtaaat cgtttccatg acaggagaca caatttgtca gaggttgatt
     3241 gagtccgctc ttatgcatat atccaccctg actgacggta tgatctgccg gaggattgta
     3301 ttttgggtca ttagtatttt cacagccaac cagaagagtg cctatgacca gtatcacaat
     3361 cactatcaat gatttgtggg ttttcatttt tcgaattttt agttgaacat taaattcaag
     3421 gacggatgcg gatgtacttc tgcactatag aacgtcctat gaaaatttta ttgtaagccg
     3481 cacagggaag ttatgaaccg ttccatactt cactggcagg catgtcaata agttaaattc
     3541 tggcacttac agcgtcagta ggcctgcttt ccgcaccgtt tttatattta actacaatga
     3601 aataatagta catttttcct ttttcagcag tgtcatcggt aaagctttcg gctttgccat
     3661 caagggtttt cagcaggctt tccttaccgt tttcaggagc acggtaaaca agtacagact
     3721 ggatatcacc cgcaaccgga agtgtccatt tcaaagttat aacttttgag gatgcgattg
     3781 ccgaaacctc acccggaggc aatgcactgc cagctggaac ataaatactg tacggcaggc
     3841 tgaaactgct cacatcagtt gtgtcagcag cgatacacct taccctgtag acgtaatttc
     3901 tgccgggcat aacctgcctg tccgtaaagc tgtttgacat gaaatttaca gttccgagaa
     3961 gttgctcatt cccgtattcc ttaccctggc tcctggtact gcgatatacc tcataggctg
     4021 tgactgacgg atttaaacca gccacatcac tccagaccag cttcaccgca ttctcttcga
     4081 gcaaggcatt cagcctgtca ggaacgggca tcaggccacc gctgaaggag atgctgacgc
     4141 gactgctcac agggcttata ttgtaggaag tgttgacaga tgccacggca tagctgtaaa
     4201 cagcagaacg gcttgaaaca ggaagcgtat cggtatatgt cagcaaactg tctgtggaat
     4261 gcagcattct ctccagctgg tgcaactcag aagtatatcc gtcggcgcgg tatacataat
     4321 atccgtggat atcataaccc actcttctga acctgagtgt gaccacgttg ccactccggg
     4381 tggctgtaag gtcctgcgga gggataatat tttccttatt cccccggaga attaccggga
     4441 cccgggcgct gggcaggctg ttgcctgtcc cgttgttcag gactgcataa tagtagtaag
     4501 ttatggaagg ctgaaggtct gagccatcaa aatactcgtt ctgaccggcc ggaagtgaaa
     4561 ccaccctctt ataattgtca ttgtatgagg tggaacggta aatctctatt gcatttacaa
     4621 aggctgcatg atcataatcc catttcagca gattgcctcc cttttcaggc tgtggcgtta
     4681 cggtaaactc cgtaacaata ccaatatctg cgggttttgt aatgaaattc acattcaccg
     4741 gttcagactg tttcccgaga ttgcccagtc catcataagg aatggcaaca taactgtaga
     4801 tcatgccggt tgttacattc ctgtctctga cgagggctac catctttcct ttttcctggg
     4861 caaacattgt ttcggcggaa atctcactga aggcattttg catatacgga cttcgctgca
     4921 gtttaagccc tgcagtgttt ccaatatctg agagttcata gcttatctcg atatagccca
     4981 tattgacctt gtaacgcaca ggtatcgcct catcagcaga tggttccggc gggaatgaca
     5041 cgctgatttc ccttccctgg gttttgcttc gcgatccgct caggcggctg atacggtatt
     5101 tgtacgttcc gggagtttta gtttcattgt caaaccatcc tgctccggca acatactgcc
     5161 atcttggatc tgtcgagtgg atataaagtg aatccagtgt agcggagggg cttttaatct
     5221 tgttccagac aaagtcagtc attccatcgc ttatttcggt aacggcagat acaacttgcg
     5281 gaagtgacat cagccttgcc cggcaatcag cggcattttt cggagcctga atctcggcaa
     5341 ctgatatcca ttttcccttc gcatcctgtt tttcgataag ccaggagaaa ttctttgcca
     5401 gttctttccc acagaatatg taattcccgg ttgcatatga ctggctttta tagttctgtg
     5461 caacagcaat ttgcataaat acgagcaatg caaacgcgat aactgtaatc ctttttgtct
     5521 tcatggaatt tcgccttatt ctttacagct gcttaattta tctgtaccgc tttccagcag
     5581 gaatctgaac ccgcctgaac cgccttccgc cgaagcgttg acatctatgc tcttggagac
     5641 cgtttttact cccaggtcct gggagatttt ggccgagaat ccaagattta ttgatgtttt
     5701 aatgtatgtt ggatctccaa gctggaattc aattctgcca tcacctccga cgctgccgct
     5761 tatgctggta ccggttatag ctgagagtcc cgccccggcg tgaaggtata cataaccgcc
     5821 aaggccaacc tgccagcttc cgccggtgac attcacatac gtattgcccc cgatcagccc
     5881 gagcgcatca acatatcctt tggccaggac ataatcaaaa tcctccctgg ctttgaacag
     5941 ttccctgtta aaggcgaaat aaaatccgga gaggcgctca gtgtttttct tgtagcacca
     6001 gttgacaacc ctgtcatcaa tataaatatt ggtcaactgc cacatctcat cggtaagctt
     6061 gtgcgatcct aacataagcc cggtattata gttcccggca agaatcccta tgggaaggaa
     6121 ggcgttgcat gctccgatta tataaaatcc ctcagccccg aagcaggaag atatagtacc
     6181 cttatgtatc acaaccgacc ccaatgtcag ttcatagtta aagctcaggg ttccgatcag
     6241 ctccttggtt ttgaagtcga atgtctgggt catcgacccg aacggggtat ttatggcgct
     6301 gacagacagc ttgtctgaac tggcggagat atctcccagg acttcaaatg tagttttggt
     6361 cggggcaata tcgtccgttt tggatttgtc attgtttgtc aggtcaccgg tgaatttaca
     6421 tgtggtccag tcgtttccgg taactgacat gcctccctcg gcaattttca ggctgtaacc
     6481 cttggatgat gccacaggtg aagtgattga ttcatcctct tcgcttgtca gctgggttac
     6541 ccagtctttc tgcatcttca cttcatagtg cggggttccg gttacacggg catagaaagt
     6601 ggaggggatg ggattaaatg tttttgacgg cttttcaatc accttccctg aaatcctgat
     6661 ttcattcttt tctatcgcta tcggcttcaa actcgaatag aaatgaacga agcctttggt
     6721 ttcaaaatcc gtactgacgt tttcaaagtc aggatttgct gtcgggcttt tccagattgc
     6781 attcagcaga atatcactta cacgcggggc tcctacattc agcgtaccgc tgagtttcat
     6841 gtagttcgga ccgttataaa tggactgagg aacgaaactg gcgagggaat tgcctttaag
     6901 ccgggctttt acaccggatt gtttcagctg aacaatcatc tcgttgttgc tcaggatctg
     6961 cagatagtca aagtcaaccg ggtatggggt caccaggtca ggatttgtaa gtcctgccaa
     7021 accgggcagg gatgccacct tttcagtgcc gttgcttatc aggttgaagt tccagtgcgg
     7081 gttcatatca gatcccactt tctgctcgaa attcagatga gccttcgtct ggtcaacatc
     7141 cttcagaggg aatatgccac cggcaagtgc aattttgtca aactggaact tatcaatcag
     7201 gaacatgtca ttacgcagca cgaaccgggt aagcggaata tccacgattt tggtgcggat
     7261 catcgcattc tggctgacaa tgcctccttc cgaagggtcg agtttccagt ctttaacctc
     7321 cagagtccaa tcctcaagtg cgagctttat cggcacggta cctgaagcag ggctcacctt
     7381 attctggtca agcactattc ccgggatgtt caccgtgaag ttttcgggct tcgcgtttgc
     7441 aatgctgcag gtcagatcca catcaaggtg tatcttgccg ttatcgtcaa taaaactgtt
     7501 aagcggattt gcggtagccg ggaaattcag cagtttgaaa gcaatcgggg cagcgttcct
     7561 gtcgcaaagg taataaacag gtttcggctt actcagataa tcaatgtacc cggtctggat
     7621 cgccttctga tacactgaca gattatcata agctggtttc gatgcttcag tttcagatac
     7681 tgcgcttgtg acaagctcca cgatattgct aattgccgaa ttctgctttt ttgccaggaa
     7741 gaattcactc ttgtcaaaat tcaggtagct gctggggtaa ttgaaggaat tgtcaacgat
     7801 cttcattttc ccgtctatct tcccgaaatc attgaccctc ttaaccgtaa gcggctgggt
     7861 gttgaaaatg ttctgtgtgc cggctgactg cagggcaatg ttgtagctgt ccagatagga
     7921 gagcttcagc attgtgactc cttttatgac aacttcatcg tcattggcta ttccaacgac
     7981 tgacccattc ttgcttacaa ggttgaaaac aacgtcttca atcctgagca acttgttcac
     8041 ctcgttgagc gagaaatcgc tgatagcctc agtccagtgt ataagccccg tcactttcac
     8101 ctgtttctga ttcacgggag taagactttc cacagtgagc ggaaagccgt agatcttatt
     8161 gattacagcc tggctgtatg atttcagatt caggtcggca actgcttttc ctccttttat
     8221 tgatgcaggt tgcttgtcgc ctgctactgt gaaggtagtg tcgagtgtgg cacggacaaa
     8281 tatttcctgg ttatcaaccg ggacaccgcg aagggtatat ttcccctggc tgtcagtgta
     8341 agccgccaca aggttggcat cctgagtgat cttaccctca ggcttgttga accccgggcc
     8401 gttaagattt acgacagggt agtcagcaga tccggacttc tggctgatat tcagtgatga
     8461 agcaacctgt gttgatgtat ttgagacatc tatatagacc tttgcgtttt tcaccggtgt
     8521 cccgtcaagc tttacgaggc cgctgatttc gcttcccttt tcaagttgca cggattttat
     8581 cacgaagtcc tttgtttcct tactgttcac atttactgtc ttagggatat atccgcttcc
     8641 ggcagcgccc cttactacga aggtgtaatt gttcacagag acgttttcga agttgaaccg
     8701 ggcaataccg cctgcatcgg tggttctgac tgaatcacca agctgaattg ttgcacccgc
     8761 aatgcttttc ggcggacccg cctggtcaag tttctcggtg accgagaact gaatcctgtg
     8821 acggcggcga aatacaagaa cgtcgttcag tttcagaagg tccttggctg cgtctgcatc
     8881 cgtgaaaacg tatgatgaat tgaaataggc aacatccttt ggtatgatct tcaatgtatc
     8941 ctttgccatg tagggagccg ggatttcaaa atatcccgaa ccataggtct caaaatattt
     9001 cccgtcattt acctgtacat atgcctgtac ggttgccaat gtgttgtccg gcagcttctc
     9061 atcagggcat ctcacggttc ctgttactgt atgggcgggt gagaggccga tatcctttac
     9121 gaactgaaca cccttcctga taaatgaata gcctgctccg gccccggagt ccctgtatcc
     9181 gttcgctccg gcaaagaaat ataccttggt cggattctga ccgtctgcaa catattcgtc
     9241 aagcgcggag cctccggaag caagtatttc ggctttgccg taatcatcca ccggaacggt
     9301 tttgaaattg ctgtttcccg agatggattg ctcttttgac agcattaccc gggcaccatg
     9361 atttttggtc acaaccgaac cggatttcac atcacggaca gtgataagtg cccggctcac
     9421 aagaggttgc aatattacat ccgcttccac gaccgggatt tccgggtttg cccagttggt
     9481 ctcagtgacc tgggggacct ggggtgattc cctgattgtg gcctgatatg tcaggttatt
     9541 ctcagccaca taactcgatg attcaatgaa gtaatacggg tactgcgact gaagaagggc
     9601 cgggagggaa gaatagttgg actgacttgc agaattgtac actgtaggtc tggcaggcca
     9661 gagttgttca aattttttgc tgaaaatgcc cccggcatct attttgtcgg aagtggaggc
     9721 tgcatcctgg gtgttgtatt gagggttcag cagctcagca taagtgtatt tcccgtctcc
     9781 ttcgccgtga ggcaggttct tctttttgtc tgccaggttc cggaaaagtg taacctgcat
     9841 gcccgagagc gaaatctttt ctttatcctc attaagggtt ttgggataaa ctttcatttc
     9901 aaattccctc acatagtggg taatgtcaat cggggtctga atttcctcca atggctgtac
     9961 agtgattatt tctgctgagg ggtaataata tgatgctgca tcgccatcga ttattatgcg
    10021 aaacactctc ttgaatccct tgtacggttt tgtttcgtat tcatcttcag cagcatatgc
    10081 aggtattgga catggattgg ttgcaggcag ggagttgact gacggattgg gtccgtgagc
    10141 cggagcgtcc ggctccggaa aaaacatctc cccgccggtt gtaatgcttt tgtctccttt
    10201 taacttgctg ccgtagagtt caaatgagtt tagtccctca tcaaagctga cattaaagcc
    10261 gggatttatt gatgactggc cactcagcga tgcattgccg gtcccgaaac cgttgccgcc
    10321 gggcttcagt cctgcaatag aaggacctga agggaatgca aagctgctcc cgtcattcca
    10381 tggattcgtc acatactggt ccattccctg aaggccggcg ccgagttctt ttttaaactg
    10441 atcgccgatg cccccgccgg gagttgtttc cggcacagag tgacttgtct ttgaatgagt
    10501 gacagtcccc gggccaaggt cgcctttaaa gtccaggttc acggtttcaa ttacgaaatt
    10561 tccgttcgca tcggtaatgc ctgctgccat tgtggcatac tgatcggata aggaaaacca
    10621 ttcatcagta tcgccacctg aattatcagg ggaaaacgaa tatgattcgc taccgcccgg
    10681 taaacctgga atgttattgt actggctctt tgtggctcct attgatacat tgttctcgtc
    10741 gacatagttt acaatcaccc tgaatcgggt atttgcaagc gggctcttca atttgccctt
    10801 gtcagatttc catgtgtaga gcaatcttcc gcgtattaca gaggttggag gccggcatga
    10861 aaccacatca taatctgcgg aaacggtccg gtaatatgca tcggggtcac ctgtttcagg
    10921 cagggccagg ctgaattcca tcggttcagc tatataatcg tcctcatcga ggaagtttgt
    10981 ggtgcttgtc cccgcagtga taatactttc aatggccgct tccagagtca tcttttccga
    11041 agcactgata ggtgtagcag ggttaaactt gtaagccgtt ttagctgcat ctgcatttgt
    11101 ctgggttgca ttgacggcac ctgttttgac cgcgtcagtc tttactgctc ccgtttttac
    11161 ggcccctgaa atgtttatat tctgattctg cttactgacc caattgtcaa tgtctttaac
    11221 cgcaatgatt ttatattttt cagaattgga tgccagcaaa cggtcgaaaa ctgcgtagga
    11281 tttgtctccc tggatctcag tgattccttt ggctataacc gtgattttcc cgtcggtgag
    11341 cggagttttc atgtcaatat ttccttccca ggccgggata tggggtttct tgttttccct
    11401 gtacaggatt accgggatgc cgggagcaac agctacaatc tctgattcat aatagccagc
    11461 ctgctgtgta tcattcagag tattttcgac cccctgggtc ttcatttttt caagggcttt
    11521 ctgggcttcg gttttttcct tgtgctggac tgtgacattg gtcccgtcct ggctcacgaa
    11581 ataggaggta aaccgttttt gcactttcag attcaacctg taagcatatg tttttgcaac
    11641 aagctctcca aaatcagcga aggagcttcg cccttccgcc catgtgagga attcccctga
    11701 accgggtgtg ccgttctggg aggagggatc ctgggatact gtatgatcct gatgggtgac
    11761 aatcttcagg tcaaattcat catccacata gtactggttt ccgatcagta aacggcaata
    11821 gatcgtgtca ttgacgatgt agttcaccgg tactggcaac tgtgaattga ttttgccctg
    11881 gtcatcagtt gtgagtgcaa ccgaagcgac ctttttataa ctcctgtttt taactgtaat
    11941 gaccggcgca ggatccgtat ttcccggata aatagggacg agtgcttctg caatcacatc
    12001 ggtacctgtc acaggataca gctccgggaa acccttgaaa ctgtatttca acacggcctg
    12061 tattgaaatg tcctgcatca gggtaccttc aagttccctg tatataaaac cggggctggt
    12121 gtaggttgct atctggttat ttgtcttgcc aatttgtctg attgaggcag tgt
//
LOCUS       DAOPXU010000081        11852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_6116_length_11852_cov_11.508773, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000081 DAOPXU010000000
VERSION     DAOPXU010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11852
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6116_length_11852_cov_11.508773"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="PKK03_08740"
     CDS             complement(<1..1191)
                     /locus_tag="PKK03_08740"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX84527.1"
                     /translation="MRRVRYIICVILVAHAVSLAGQTKSSLRELFVSAEGDLLFEDYA
                     EALPKYMSLLQIYPENYNLYYRIGQCYLNTPGEKDKAISWLETAAGHINPEYRGGRLR
                     ETGAPYDALYFLANAYRIANDFDRALETYDLFLKDVDTEKYDTALVRFQMQTCHNARE
                     MMRKPVFITDRNLGSTINDRFSESNPVISNDENTLLFTRELQFYDAIFWSRNVDGQWT
                     EPVNLTPQLGIDQDYYTSSLSADGRTLLMYRTDTYDGNIYLSRLEGDTWTKVEKLNGN
                     VNTRYWESHATMSSDGKKLFFTSNRRESIGGLDIFVSARDSTGNWGPALNIGPGINTV
                     YNEETPFLANNDSTLFFSSRGHFNMGGYDIFRSDMGPDGQWSAPVNIGYPLNTSDDDL
                     FFTPV"
     gene            complement(1280..2140)
                     /locus_tag="PKK03_08745"
     CDS             complement(1280..2140)
                     /locus_tag="PKK03_08745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX84528.1"
                     /translation="MELSAVIVSYNVCELLRGALQSLMAAAKATEHEIIVVDNNSSDG
                     SPGMVSQEFPSVKLIVSDSNEGFASACNRGIQASSGEYVLILNPDTVTSPDAITRALD
                     FMKTHPDAGAAGAHMTDGNGKFLPESKRGFPSPLTALFRFTGLCRLFPSSALFNAYYL
                     GDKPENETCRADILTGAFMLIRRKALDKAGLFDTSFFMYGEDIDLSWRIRKAGFSNYY
                     LHDVRITHFKGGSSGQDRQTSVWHFWDAMAIFGEKHLQKGWHMPVRAAVSLLKWLSIT
                     RLRVAGLVKK"
     gene            complement(2145..3359)
                     /locus_tag="PKK03_08750"
     CDS             complement(2145..3359)
                     /locus_tag="PKK03_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNX84529.1"
                     /translation="MADTFDRFFSDASHLMKKSAIREILKLTQRPEMISFAGGLPSTD
                     SFPIEDIRRVTSEVLEQDGKSALQYGTTEGDNKLRTLLAERHRKDGLEIGPENIIITT
                     GSQQALDLCGKIFVNRGDVVLCGLPSYLGGLNAFANYGALLKGIPLDDQGMRPDLLEK
                     TITDLKKEGKIIKFIYIIPDFQNPTGVTLPRSRRLEIIRIAERHDLLIVEDSPYRDVR
                     FAGTPEPLMSSLDTHGRVMTLNTFSKILAPGFRLAWVTGHREVIDKMVTAKQSADLCT
                     PPFVQKIAARYIEQGLLETNLRKTITLYHRRRDFMLKCFRELMPEGVKWTEPDGGLFL
                     FVTLPPATDAARLLEKAIRRNVAFVCGSVFYCNNEGHNTMRLNFSYADEKDTLEGIRR
                     LAEVIREEMATP"
     gene            complement(3530..4153)
                     /gene="recR"
                     /locus_tag="PKK03_08755"
     CDS             complement(3530..4153)
                     /gene="recR"
                     /locus_tag="PKK03_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNX84530.1"
                     /translation="MSSRFPSRLLEEAVNEFASLPGIGRKTAFRLVMHLLRRDSGEVR
                     RFGETVIRLRDDVCYCSVCHGISDTPLCQICSDQSRDISAICVVENVQDVMAVENTRQ
                     FRGLYHVLGGIISPVDGIGPSDLTINSLEERVRSGSVREVILALSTTMEGDTTNFYIY
                     KRLSGYDVRITTLARGVAIGDVLEYTDEITLGQSIVNRRLFSLPSQG"
     gene            4260..5726
                     /locus_tag="PKK03_08760"
     CDS             4260..5726
                     /locus_tag="PKK03_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNX84531.1"
                     /translation="MTPTLILLIFIAYTLLLFAITFITARRANHQSFYIGNKASPWYV
                     VAYGMIGASLSGVTFMSVPGWVGETQFSYMMVAFGYFFGYVVIALVLLPLYYRLKLTS
                     IYGYLEQRFGFWSYKTGAGFFILSRVLGASLRMFLVINVLQIFVFDAWNIPFWVNVLI
                     FIALIIIYTLKGGIRTIIWTDTLQTTFMLLAVVLSVFFISRELGEPVQKLMRNLFDSD
                     MSRIVITDWDHPRHFIKQFFSGMFITITMTGLDQEMMQKNLSIRRLKDAQKNMFTFST
                     INLVVVFMFLVLGAFLVFFARAESINVAMTDDLFPTIAFRYLSPAAGIVFLIGLISAA
                     FPSADGALTSLTTSFSIDFLGLEKKTEMDNARKTRIRYTVHFSFALIFFICILAFRQM
                     NDRAIIDKLFTIAGYTYGPLLGLYTFGLFTKRTVTDRFTPWIAIISPVLCYFLNVFSE
                     ELFNGYRFGFEMLLVNGFLTFMGLLIFSKKGSKTVAEA"
     gene            complement(5703..6470)
                     /locus_tag="PKK03_08765"
     CDS             complement(5703..6470)
                     /locus_tag="PKK03_08765"
                     /inference="COORDINATES: protein motif:HMM:NF039966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HNX84532.1"
                     /translation="MARRTCIFFISLFLLAETVAVSQDTVTVPLHVRAGFDISGPVMK
                     LVTKDLISYGVAASADINSSIAATAGVRYSSYRSSDVLYDFMSRGFSFTAGADYNFIK
                     ANVSQGRYSAGIGLRYGLSFYREEAPMLRYTNEWGAGETSVPAESHTGHFMEITPGVR
                     AELFRGVTIGWNLYMRLLLSAGAGKDLKPVWMPGYGPGTSGMTTGAEYYVSISIPYRK
                     IKVYIKPKVRETEEEETEVTNTGSGTGTGLSGFSNRL"
     gene            complement(6439..6906)
                     /locus_tag="PKK03_08770"
     CDS             complement(6439..6906)
                     /locus_tag="PKK03_08770"
                     /inference="COORDINATES: protein motif:HMM:NF040109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HNX84533.1"
                     /translation="MKRLSVIIYIAVIPSLVMVIPSCSVQPCYEETDPVLHTVLLVSG
                     TGEAAKADSLRVYTYRETDTLRFINLKGVSSFSVPLDPASDESEFLITLNGITDTALI
                     RYSRKPHLVSPECGYTIISELTGLKTTHNIIDTLIIENKFVNLDGQKNLHLFY"
     gene            complement(7109..8962)
                     /locus_tag="PKK03_08775"
     CDS             complement(7109..8962)
                     /locus_tag="PKK03_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNX84534.1"
                     /translation="MEVTRTFDLIDLGCKVCPREVMFGGKHGNGWVTYSTEAVRELTD
                     LFSCGMMAIGYTRGDHIATITPNKAEWNIVDHGLAQAGMIHVPVYPTIGHEEYAYILD
                     HSGAKAVIIGNKQIYNKISSVVSKIPAVRALYSFDQFNGLMTFDDIIEEGRKNRGRFM
                     DELSAIRDSIKPSDMVTIIYTSGTTGNPKGVMLSHNNLVTNAIATSTAHEFGLGYRAL
                     SFLPLCHVYERMMNYHFQYKGTSIYYLENMGVIAETMREIKPHIANTVPRLLEKIYDN
                     IISKGKNLPWLKKQIFFWAVTLGLKFRLRGNTRFYRFRLGIARKLVFNKWKEALGGEL
                     RVLVSGGAALQSRLERIYWAAGIPVLQGYGLTETSPVIAVNPLRIPDIKFETVGPILD
                     GVQVKIADDGEILCKGPNIMMGYYREPEMTAEVIDSDGWFHTGDIGRIEDGRFLRITD
                     RKKEMFKLSAGKYISPQVIENRLKESIFIEQAMVIGENEKFASALLSPNFTFLHDWCS
                     IHRIQYRDNTDLVEIPEVIERFAREVREVNKNLGEFEQIKRFRLVTEEWTPQTGELSP
                     TLKLRRNILTGRYQNIINEIYSVKEKGNVVEKVVEVIRNGVSGILKNLPKF"
     gene            complement(9119..11752)
                     /gene="alaS"
                     /locus_tag="PKK03_08780"
     CDS             complement(9119..11752)
                     /gene="alaS"
                     /locus_tag="PKK03_08780"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNX84535.1"
                     /translation="MRANEIREEFLGFFRSKEHKIVPSAPMVIKNDPTLMFTNAGMNQ
                     FKDIFLGNQPAKYKRIANSQKCLRVSGKHNDLEEVGLDTYHHTMFEMLGNWSFGDYFK
                     KEAIEWAWEFLSGRLGIPAGRIYATVFEGDPVEDVPRDEEAATYWAKCFGNTENHILD
                     GSKKDNFWEMGETGPCGPCSEIHVDIRSESERAAIPGHLLVNKGHPQVIEIWNLVFIQ
                     YNRRAGGELELLPDRHVDTGMGFERLCMVIQNKQSNYDTDVFQPLLAASAEATGKPYG
                     QKNDWDIAHRVIADHLRTVSFSVADGQLPSNNRAGYVIRRILRRAVRYGYTSLGMTEP
                     FIYRLLPALVESMGGHYPELKAQQDIISKVIREEEQSFLRTLGKGLRMIERRVETLRQ
                     EGKSIFPGSDAFELFDTYGFPVDLTQLILRENRMTLDEEAFRNELNAQKERSRDDAAV
                     SADDWVVVRETESTIFTGYDRTEDQVRVARYRKVTSRGRQAVQLVFDRTPFYAESGGQ
                     TGDRGFIEAGKEKIIITDTVRENNLIIHIASAVPDDPSLTFKAAVDAEARQDTANNHS
                     ATHLMHHALREVLGKHVEQKGSLVTPDRLRFDFSHFRQVTPEEISAVEEKVNSMIMQN
                     IVSEVHEDTPIEEALSMGAMALFGEKYGEKVRVVTFGDSVELCGGTHVDRTSRIGLFK
                     IVSEGAVAAGVRRIEAVTGRKAVSYINEKLSILDEVTAMLRSTGNPVANISKLMAENA
                     ALKGTIEKLQASAALSARNELAARAETAGNCRIYSGVLEETGADTLKNIAHQIRQADS
                     SSIIVIGSGYEGKAALVVGLGDNATGSGRLNAVEIIKAVSGEINGGGGGQPFLATAGG
                     KKPEGLPGAVARAVEMAKKML"
BASE COUNT         2621 a         3072 c         3032 g         3127 t
ORIGIN      
        1 cacaggggtg aagaacaggt catcatcaga ggtgttcagc gggtaaccta tgtttaccgg
       61 ggcgctccac tgcccgtcag gccccatgtc tgacctgaag atatcatatc ctcccatgtt
      121 gaaatgtccc cgggagctga agaacagggt gctgtcatta tttgccagga acggggtttc
      181 ctcgttataa accgtattga ttccgggacc gatattcagt gccgggcccc agttgccagt
      241 gctgtcgcgg gccgagacga agatatcgag accgccgatg ctttcccggc ggttacttgt
      301 gaaaaagagc ttcttcccgt cactgctcat tgtggcgtgt gactcccagt acctggtatt
      361 cacattgccg ttaagttttt caaccttggt ccaggtatcc ccctccagcc ggctcaggta
      421 gatatttccg tcataagtgt cagttcggta catgaggagg gttcgcccgt cagcggatag
      481 ggatgaggtg taataatcct ggtcaatccc cagttgcgga gtcaggttga ccggttctgt
      541 ccattggccg tcaacattcc gtgaccagaa tatggcatca tagaactgca attcacgtgt
      601 gaacagcagt gtgttttcat cgtttgaaat taccgggttg gattccgaga atcggtcatt
      661 gatcgttgat ccgaggttac ggtcagtgat gaacaccggt tttcgcatca tctcccgggc
      721 gttgtgacag gtctgcatct gaaatcttac cagggcagtg tcgtactttt cggtatcaac
      781 atccttcaga aagagatcat acgtctccag tgccctgtca aagtcattag cgatacggta
      841 tgcatttgcc aggaaataga gggcgtcata cggtgccccg gtctcgcgca gccttcctcc
      901 ccggtactcc gggtttatat gcccggcagc agtctcaagc caggagatag ccttgtcctt
      961 ttcacccgga gtattaaggt aacactggcc aatgcggtaa tagagattat aattttcggg
     1021 ataaatctgt agaaggctca tgtatttggg aagggcttct gcgtaatctt caaagaggag
     1081 gtccccctcg gcagagacga agagttcgcg caggctgctt ttcgtctgac cggcgaggga
     1141 tacagcatga gctacaagga tcacacaaat aatatatcta acccttctca tcaggttgat
     1201 aaatattctt taagtaacta atataactca aattctgcta taagattata gtagaacagg
     1261 tcctttttaa tagggaattt cactttttga ccagtccggc cacccgcagg cgggtgattg
     1321 acagccattt gagaagactc acggccgccc tgacgggcat gtgccacccc ttttgaaggt
     1381 gcttctcccc gaagattgcc atggcgtccc agaaatgcca gacagaggtt tgcctgtcct
     1441 gacctgaact gccccccttg aaatgggtga tgcggacatc atgaaggtaa taattgctga
     1501 accccgcctt ccttatacgc cagctgaggt caatgtcctc accgtacatg aagaaggagg
     1561 tgtcaaacag acctgcctta tcgagggctt tcctccggat gagcataaaa gcaccggtga
     1621 ggatgtctgc acggcaggtt tcgttctcag gcttatctcc caggtagtat gcattgaata
     1681 aggcagaact cggaaagagc ctgcataggc cggtaaacct gaatagtgcg gtcagggggg
     1741 atgggaagcc cctttttgat tcaggaagga atttgccgtt gccatctgtc atgtgtgcac
     1801 cggccgctcc tgcatcagga tgtgttttca tgaaatcaag ggccctggtg atggcatcgg
     1861 gactggtgac agtgtcagga ttcagtataa ggacgtactc tccggacgaa gcctggatac
     1921 ccctgttgca tgctgaagca aatccttcat tgctgtcaga aacgatcagt tttaccgaag
     1981 gaaactcctg gctgaccatt cctggagagc cgtctgaaga attgttgtcg acaacaatga
     2041 tttcgtgctc tgtcgccttc gcggcagcca tgagcgactg cagcgcaccg cggagcagct
     2101 cgcagacatt gtaggaaaca attactgcag agagttccat cgggtcatgg ggtggccatt
     2161 tcctccctga tcacttccgc cagccggcga ataccttcaa gggtatcctt ttcatctgcg
     2221 taggaaaaat taagacgcat ggtattatgg ccttcgttgt tgcagtaaaa gactgatccg
     2281 cagacgaaag ccacgttcct gcgaatggct ttctcgagca gccgtgcggc atccgtggca
     2341 ggaggaaggg tcacaaagag gaaaagtccg ccatcaggct cggtccattt aacaccctcc
     2401 ggcatcagct cacggaaaca tttcagcatg aaatcacgcc tccggtggta gagcgttatc
     2461 gtcttcctca ggttggtctc aagcagtccc tgttcaatgt acctggctgc tatcttctga
     2521 acgaagggag gggtgcagag gtcggctgac tgttttgctg tcaccatctt gtcaatgacc
     2581 tccctgtgac ctgtcaccca ggccagcctg aaacccggtg caagtatctt tgagaaggta
     2641 ttgagggtca ttaccctgcc atgcgtgtca agggaagaca tcagcggttc aggagtccct
     2701 gcaaaacgga catcgcggta cggactgtcc tctacaataa gtagatcatg cctttcggcg
     2761 atgcgaatga tctcgagacg gcgcgacctg gggagagtga cgcctgtagg attctgaaag
     2821 tcgggaatta tatagatgaa cttgatgatc tttccttcct ttttcaggtc ggttatggtt
     2881 ttttcaagga ggtcaggacg catcccctgg tcatcaagag gaatcccctt cagcagggcc
     2941 ccgtagttgg cgaaggcgtt gagcccgccc aggtatgaag gcagtccgca cagcaccaca
     3001 tcaccgcggt tgacgaaaat ctttccgcag aggtcaagcg cctgctgtga gccggtagtg
     3061 attatgatgt tctccggtcc gatctcaaga ccatctttcc ggtgccgttc ggcaaggaga
     3121 gtccggagtt tgttgtcacc ctcggtggtg ccatactgga gagctgattt gccgtcctgt
     3181 tcaagtacct ccgaggtcac tctgcggatg tcttctatgg ggaaagagtc agtcgagggg
     3241 agacctccgg caaaggagat catctccggt cgctgcgtca gcttaagtat ctccctgata
     3301 gccgatttct tcatcagatg cgatgcatct gaaaagaacc tgtcaaatgt gtcagccatg
     3361 ctaatattga tttgtatcaa attaagcaag attttcagaa taaaaaaatg acagtggccg
     3421 gcaggctgaa aaagttactt ccggacagac agggaaggtt gtttccggac agacagggaa
     3481 agtcactgca taggacgtgc ctgtcacggc cggcccggga gcatacccgt cagccctgtg
     3541 acggaaggga gaaaaggcgg cggttgacta tcgattgccc gagggtgatc tcatcggtat
     3601 attcgagaac atctccgatg gccacaccgc gtgccagggt agttatacga acatcatagc
     3661 ccgacagccg cttgtagata tagaaattcg tggtgtcacc ctccatggtt gtgctcagcg
     3721 caagtatcac ctccctgaca ctgccgctgc ggaccctttc ttccaggctg ttgatggtaa
     3781 ggtcagaagg accgatgccg tcaaccggtg aaatgatgcc tccaagcaca tggtacaggc
     3841 ccctgaactg ccttgtattc tccacagcca tcacatcctg tacgttttcg accacgcaga
     3901 tggcagagat gtccctcgac tgatcactgc atatctggca cagcggagta tcactgatgc
     3961 catgacagac gctgcaataa catacatcat ccctcaggcg gatgactgtt tcgccgaaac
     4021 ggcgcacttc cccgctgtca cgcctgagca ggtgcatgac aagcctgaag gctgtcttgc
     4081 ggcctatccc gggaagcgag gcaaactcat tcacagcctc ttcgagcagt cttgaaggga
     4141 aacgtgatga cataatacga ctttgtgaca aaaataattc aatatttgcc gtaaacctga
     4201 cagcttttaa tttttatctg cgatcttttt agtattcttg cagatacgtt tgcagaatta
     4261 tgacacctac cctcatcctg ctgattttca tagcctatac gctcctgctg tttgccatca
     4321 ccttcattac tgcacgaaga gccaatcatc agtcgttcta tatcggaaat aaagcatcac
     4381 cgtggtatgt tgtggcttac ggcatgatcg gagcctccct gtcaggggtg accttcatgt
     4441 cagtgcccgg atgggtggga gagacccagt tctcatacat gatggtggcg ttcggttact
     4501 tcttcggata cgtggtgatt gccctcgtgc tccttcctct ctattaccgc ctgaagctta
     4561 cctccatata cggttacctg gaacagcggt tcggtttctg gtcatacaag accggggccg
     4621 gattctttat tctttcaagg gtcctgggtg catcactccg tatgttcctt gtaataaatg
     4681 ttctgcagat ttttgtattt gacgcatgga atattccctt ctgggtaaac gtgctgatat
     4741 tcattgccct gataatcata tacaccctca agggggggat tcggaccata atctggacag
     4801 acacacttca gaccacgttt atgctgcttg cggtggtgtt gtcggtcttt ttcatcagcc
     4861 gtgaactggg agagcctgtg cagaagctta tgaggaacct gtttgacagt gacatgtcac
     4921 ggatagtgat tactgactgg gatcacccgc gccatttcat caagcaattc ttcagcggga
     4981 tgttcataac cattaccatg accgggcttg accaggagat gatgcagaag aacctcagta
     5041 tcaggcgtct gaaggatgct cagaagaata tgttcacctt cagcaccatc aatcttgttg
     5101 tggttttcat gtttctggtc ctcggcgcat tcctggtctt ctttgccagg gctgagagca
     5161 tcaatgtggc aatgactgat gatctcttcc cgacaattgc attcaggtat ctcagtcctg
     5221 ccgccggcat agtcttcctt atcggactga tctcagctgc cttcccgagt gcagacgggg
     5281 ctttgacatc gctgaccacc tccttcagca tagacttcct cggccttgag aaaaagactg
     5341 agatggataa tgccaggaag accaggatca ggtataccgt gcacttttcc tttgccctga
     5401 tcttttttat ctgcatactg gccttcaggc agatgaatga cagggcgata attgacaagc
     5461 tgttcaccat agccggatac acctacgggc cgctgctcgg cctgtacacc ttcggtctct
     5521 tcacgaagag aaccgttacc gaccgcttta ccccgtggat agccatcatc tcaccggtat
     5581 tatgctattt cctgaatgtc ttttcagaag agctcttcaa cggttacagg ttcggctttg
     5641 agatgttgct ggtaaacggc ttccttacat ttatgggatt acttattttc agcaagaagg
     5701 ggtcaaagac ggttgctgaa gcctgataag cccgtgccgg ttccgctgcc ggtgtttgtt
     5761 acctcagtct cttcctcctc cgtttcgcgg actttaggtt tgatataaac cttgattttc
     5821 ctgtatggga ttgatattga tacataatat tctgcaccgg ttgtcatgcc tgatgtaccc
     5881 ggaccgtagc ccggcatcca gaccggttta agatcctttc cagctccggc gcttaacagg
     5941 agcctcatat aaagattcca gccgattgtc actccccgga aaagctcagc gcggacaccc
     6001 ggggtgatct ccatgaaatg acctgtgtgg ctttccgcag gaacagaagt ttctccggct
     6061 ccccattcat ttgtatacct gagcataggg gcttcctccc tgtagaaact gagtccgtat
     6121 ctgaggccta tgcctgcact gtacctgccc tgtgatacat tggccttgat gaagttgtaa
     6181 tctgctccgg cggtgaagct gaagcccctg ctcatgaagt cgtacaacac atctgatgaa
     6241 cggtacgatg agtacctgac gcctgcagtt gccgctattg atgagttgat gtcagccgat
     6301 gctgcaacgc catagctgat aaggtctttg gtgacaagtt tcatgaccgg gccggagatg
     6361 tcgaatcctg cccttacgtg gagcggcacg gtaaccgtat cctgtgacac ggcaacagtc
     6421 tccgcaagca gaaaaagact aataaaaaag atgcaggttc ttctggccat cgaggttcac
     6481 gaatttgttc tctatgatga gcgtgtcaat tatattatgc gttgttttaa gcccggtcaa
     6541 ttcgcttatg atggtgtagc cgcactccgg cgagacaagg tgcggcttcc ggctgtagcg
     6601 tatcaaagcc gtatcggtga taccgttgag ggtaataagg aactccgact cgtcgctggc
     6661 cgggtcaaga ggcacggaga atgaggatac tcctttcagg ttgatgaatc tgagtgtatc
     6721 ggtttccctg tatgtatata ccctgagtga gtctgccttt gctgcctctc cggttccgct
     6781 gaccagaagg acggtgtgaa gtaccggatc cgtctcctcg tagcatggtt gcaccgagca
     6841 ggagggaatg accatgacca gggacgggat gacggcaata tagattatga cggagagtct
     6901 cttcataacg ggatacaaag gtataaaaat aataaggagg taaccattga atggtatccc
     6961 ctcacacact gccggaaaat tggcatggcc ggattgacgg ggccggtcag gcagggccgg
     7021 attgacggtg ccggattgac ggtgccggtc aggcagggcc ggactggcgg ggccggtcag
     7081 gcaggtctga agtggctgac ggaggctgtc agaatttcgg caggttcttc aggatgccgc
     7141 tgactccgtt gcggattact tccacaacct tttcaaccac gttacccttc tccttcaccg
     7201 agtaaatctc atttatgatg ttctgatacc tgccggtcag gatatttctc cggagtttca
     7261 gggtgggtga gagttcgcct gtctggggag tccactcttc ggtcaccagc ctgaaccgtt
     7321 ttatctgttc aaactctccg aggtttttgt tcacctcccg tacctccctg gcaaaccgtt
     7381 cgatcacctc gggtatctcc accaggtcgg tgttgtcacg atactgtatc ctgtgtatcg
     7441 agcaccagtc gtgaaggaag gtgaagttgg gcgataacag ggcgctggca aacttttcat
     7501 tttcacctat caccatcgcc tgttcgatga aaattgattc cttcagtctg ttttcaatca
     7561 cctggggaga gatgtatttt ccggccgaaa gtttgaacat ctccttcttg cggtcagtga
     7621 tccggaggaa cctgccatcc tcaatccttc cgatatcgcc ggtatggaac cagccgtctg
     7681 aatcaatcac ttcagctgtc atctccggct ccctgtagta gcccatcatt atattggggc
     7741 ccttgcagag gatctcaccg tcatcggcta tcttcacctg cacgccgtca agaatgggac
     7801 ccaccgtttc aaactttatg tcagggatcc gcaggggatt gacagcgatg acaggggatg
     7861 tctccgtgag gccgtaaccc tggagaacag gtatgccggc agcccagtaa atgcgttcga
     7921 ggcgcgactg cagggcggcg ccgccggaga ccagaacgcg gagctcacca cccagcgcct
     7981 ctttccactt gttgaaaacc agtttgcggg ctatgccaag cctgaaccgg taaaaccggg
     8041 tgttgcccct gagcctgaat ttaagtccca gggtcactgc ccagaagaag atctgctttt
     8101 tgagccaggg caggtttttc cccttactta taatgttgtc gtaaatcttt tccagcagcc
     8161 ttgggaccgt atttgctata tggggtttga tctcacgcat tgtttctgcg atgacgccca
     8221 tgttctcaag gtagtatatg cttgtcccct tgtactggaa gtggtaattc atcatccgct
     8281 catagacatg acagagaggc aggaaactga gcgccctgta gcccagtccg aactcatgtg
     8341 cggtactggt tgctatagcg ttggtgacca ggttgttgtg tgacagcatg acccccttgg
     8401 gattcccggt ggtacccgag gtatatataa tggtcaccat gtcagagggt ttgatggagt
     8461 ccctgattgc tgacagttca tccatgaatc ttccccggtt ctttctgccc tcttcgatga
     8521 tatcatcaaa tgtcatcagg ccgttgaact ggtcaaagga gtacagcgcc ctgacagccg
     8581 ggatctttga aactaccgat gagatcttgt tgtaaatctg tttgttgccg attatcactg
     8641 cctttgcccc ggagtgatcg agaatgtatg catattcctc atggccgata gtgggataga
     8701 cgggtacatg gatcataccg gcctgtgcca gtccgtggtc cactatgttc cattcggctt
     8761 tattcggggt gatggtggcg atgtgatcac ccctggtgta accaatggcc atcatgccac
     8821 aggaaaaaag gtcggtcagt tcccttacgg cctcggtaga gtaggtgacc cacccgttgc
     8881 cgtgtttacc gccgaacatg acctcccgcg gacagacctt acaccccagg tctatcaggt
     8941 caaacgttct ggtaacttcc atcaggatag gatttggatt agtgatacaa gattatgaat
     9001 ttcgaacaaa aaaaacaaga ataagaacgg tcaaattatt gacagtctaa caatttgatc
     9061 tttagtcttg tactaaaacc tgttttcagt gcctgtttcg aacggcagaa aaactcttct
     9121 acagcatttt ttttgccatt tccacggccc tggccactgc cccgggaagc ccttcaggtt
     9181 ttttgccgcc ggcagttgcc aggaatggct gtcctcctcc tcctccgttt atttcacccg
     9241 agacagcctt aatgatttcg acagcgttga ggcggccgga accggttgcg ttgtctccca
     9301 gtcccaccac cagtgcagcc ttgccttcgt atccggaacc gatcacgatt atgctgctgc
     9361 tgtcagcctg tcttatctgg tgagcgatat tcttcagtgt atcagccccg gtctcttcaa
     9421 ggactcctga atagatacgg cagtttccgg cagtttccgc cctggccgcc agttcattcc
     9481 gggctgacag ggctgctgaa gcctggagtt tttcaatagt gcctttaagt gcggcgttct
     9541 cagccatcag ttttgatata ttggccaccg ggtttcctgt gctgcggagc atagccgtga
     9601 cctcatcgag gatgctcagt ttctcgttga tatagcttac tgccttccgg ccggtcacgg
     9661 cctctattct tctgacgcct gcggccacgg ctccttcaga gacaatcttg aacaatccta
     9721 tccgggatgt ccggtcgaca tgtgttcctc cgcacagctc gactgaatct ccgaatgtta
     9781 ccacccttac cttctcgccg tatttctctc cgaacagggc catggcgccc attgaaaggg
     9841 cctcttctat aggggtgtct tcatgcacct cggagacgat attctgcatt atcatagagt
     9901 tgaccttctc ttccacggca gatatttctt ccggagtcac ctggcggaag tgactgaagt
     9961 cgaacctcag ccggtcaggg gtgaccagcg aacctttctg ttccacatgt ttgcccagga
    10021 cttccctcag cgcatggtgc atgaggtgtg ttgccgaatg gttgttggca gtatcctgcc
    10081 tggcttcggc atcaacggct gctttgaacg tcagtgaagg atcatccggc acagcggagg
    10141 caatatggat gatcaggttg ttttccctga cagtgtcagt gatgatgatt ttttcctttc
    10201 cggcctcgat gaatccgcgg tcacctgtct ggcctcctga ttcagcatag aacggtgttc
    10261 tgtcaaacac aagctggaca gcctggcggc cacgtgaggt caccttccgg taacgtgcta
    10321 ctcttacctg gtcttcggtc cggtcatatc ctgtgaagat ggtgctttcc gtttcgcgga
    10381 ccaccaccca gtcgtctgct gacacggcag cgtcatccct tgacctttcc ttctgggcat
    10441 tgagttcatt cctgaatgcc tcctcatcga gcgtcatcct gttttccctg agaatcagct
    10501 gggtgaggtc aaccgggaag ccataggtgt caaaaagttc aaatgcgtca ctgcccggga
    10561 agatgctttt cccttcctgg cggagggttt ccacccggcg ttctatcatc ctgagtcctt
    10621 tgccaagggt tctgaggaat gactgttcct cctcgcggat cacctttgat ataatatcct
    10681 gctgggcttt gagctccgga tagtgaccgc ccattgactc caccagtgcg ggcaggagcc
    10741 tgtatatgaa cggttcggtc attccgagag aggtgtatcc gtaccttacg gcacgacgaa
    10801 gtattctcct gatgacataa ccggccctgt tgtttgaggg gagctggccg tcagccactg
    10861 agaaagatac cgtccgcagg tggtccgcaa tgacccggtg ggctatatcc cagtcattct
    10921 tctgtccgta tggtttgccg gttgcttctg cggaggctgc cagcagaggc tggaagacat
    10981 ctgtgtcata gtttgactgt ttgttctgga tgaccatgca gagtctctcg aatcccatgc
    11041 ccgtatccac gtgcctgtca gggaggagct caagttcacc gccggcacgt cggttatact
    11101 ggatgaacac caggttccaa atctcaatca cctggggatg acccttgttc acaagcaggt
    11161 gcccggggat tgcagccctc tcactctctg acctgatatc gacgtgaatc tccgagcagg
    11221 gtccgcacgg acccgtctca cccatctccc agaaattgtc cttctttgat ccgtcgagaa
    11281 tgtgattctc cgtgttgcca aaacacttcg cccagtatgt tgcggcctct tcatcgcgcg
    11341 gcacgtcttc aacgggatca ccttcgaata cggtggcgta tatccgcccg gcaggaatcc
    11401 cgagccggcc ggaaaggaac tcccacgccc actctattgc ttccttttta aagtagtcac
    11461 cgaatgacca gttgccaagc atctcgaaca tcgtatggtg gtaggtgtca agtcccacct
    11521 cctcgaggtc attgtgttta cctgagacac gcaggcattt ctgcgagttt gctattcttt
    11581 tgtattttgc cggctggttg ccgagaaaaa tgtccttgaa ctggttcatt ccggcattgg
    11641 tgaacatcag tgtggggtca ttctttatta ccatcggtgc cgaaggcacg attttatgtt
    11701 cctttgacct gaaaaagcca agaaattcct ctcttatttc gtttgctctc attttaactt
    11761 cgcggcccga tttgaaacta ttttcactgg ggtgcgtaaa attaaatcaa atattttacg
    11821 tttgcagaat agtcatctta aaatggtggg gg
//
LOCUS       DAOPXU010000082        11784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_6168_length_11784_cov_13.921562, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000082 DAOPXU010000000
VERSION     DAOPXU010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11784
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6168_length_11784_cov_13.921562"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..694
                     /gene="radC"
                     /locus_tag="PKK03_08785"
     CDS             17..694
                     /gene="radC"
                     /locus_tag="PKK03_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNX84536.1"
                     /translation="MKITDWAVEDRPRERLWNKGPSSLSDAELLAILIGSGTRNNSAL
                     DLARELLAMAGNSLGQLGRLSAGEIRKIKGMGEAKAVTIAAALELGRRRKMAEAADNP
                     QIRSSADVFSIISPLMEDLSHEEFWILFLNRANRVTGRMKISQGGVSGTVTDVRIVMK
                     KAIETLASGLVICHNHPSGNTSPSDSDIRITQKIKEAGALMDIQLLDHLIVAGKDYYS
                     FADNGAM"
     gene            complement(784..1296)
                     /gene="folK"
                     /locus_tag="PKK03_08790"
     CDS             complement(784..1296)
                     /gene="folK"
                     /locus_tag="PKK03_08790"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNX84537.1"
                     /translation="MKLSACSEVIAGLGSNLGDRYAALQRALELLGEEAGEITALSSV
                     WETEPWGFDADEKFLNMVAVIKTSRRPAQLMQLFRSIEGRMGRKRSGGGKYESRIIDL
                     DILLWEERVISIPGLEVPHPKIADRRFVLEPLSEVAPAAVHPVIGLTVAEMLQLCADN
                     SDVRLAARQL"
     gene            1368..3137
                     /gene="sppA"
                     /locus_tag="PKK03_08795"
     CDS             1368..3137
                     /gene="sppA"
                     /locus_tag="PKK03_08795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNX84538.1"
                     /translation="MKTFLKMVLAVIAGIIITSILFFVVMLSVFSGMAAAGKKSVVIP
                     EKSVLVIKTGAMIPDRSQSNPLAMLDPVTMSFTQTPGLNELLSNLKKAAEDEKVSGIL
                     IENGTMPSGWATADELRSALLEFKKSGKFIYSYSDYLLMQESYFISTAADAIWINPTS
                     MFDFKGLAAEVTFYKDALEKLGVDVQVIRHGKFKGAVEPYMLDRLSDENRLQISGYMG
                     SIWEHVVKSVSESRGVPAEKIMSLADSLAGYDAALMTAAGLIDGTIYRDQLEDSIRTA
                     AGIDSGKKINYVSMSKYSNVTVKHSTVTGKDKIAVLFAEGSIVMGEGDDTNIGGNRYA
                     EELRKLRLDSTVKAIVFRVNSRGGNAIASDQIWREVELTDKVKPVVVSMGNYAASGGY
                     YIAAAGRKIIASPVTITGSIGVFGLIPNAQTLLDKKLGISSDVVRTNAHSDSPSLTRP
                     LSAFEKDALQDNVERTYNTFTGVVAAGREMSQPAVDSIGQGHVWSGADAIPIGLVDSF
                     GGLRDAISEAATLAGLEEYRTIEKPESADLYTKLLKELAGGIRAKAIRQELGEVSRYY
                     FDLKDIISSGGIQAAMPYYLEIR"
     gene            3139..4203
                     /gene="lpxK"
                     /locus_tag="PKK03_08800"
     CDS             3139..4203
                     /gene="lpxK"
                     /locus_tag="PKK03_08800"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNX84539.1"
                     /translation="MIMPGKLFLWAVSKIYGMAVAVRNRLYDLGIFRSHSFSVPVICV
                     GNITAGGTGKTPHVIYLAEKLSSPGSVAVLSRGYLRRSRGFMRVTADSTTAASGDEPL
                     LMARSLPQAQVFVDRDRVNGIREIMLTDPGTRVVILDDGFQHRAVRAGMNIILTDFNR
                     LMTRDRLLPLGMLREHIKAVRRADAVIVTKTPEVTSPSVREEIRRELIAAGPCETIFF
                     TSLAYGRPLHLYSENAGAIRRTTSVLLVTGIANPAPLEEYLGSIAGHVNHLAFPDHHA
                     YNTADIGRITSAFNAMSGPDKIIVTTAKDGVRLKEITNIADHVRQALHYLPVRVQFIE
                     DEELFLNKVYSYAGKDYQNR"
     gene            4175..4984
                     /locus_tag="PKK03_08805"
     CDS             4175..4984
                     /locus_tag="PKK03_08805"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNX84540.1"
                     /translation="MLEKIIKTAEFLASKGIKDPDAGIVLGTGLGGLTAEIDIQVEIP
                     YKDIPDFPVSTVQGHAGKLIYGDFGGKKVVAMKGRFHYYEGYGSEQVTFPIRVMKELG
                     IRALFLSNAAGGVNPNFRIGDIMIIDDHICLVDNPLIGPNDDRIGPRFPDMSEPYDKK
                     LIEKAKAIATGLNIPVTSGVYLSTTGPTFETPAEYRFFRIIGADAVGMSTVPEVITAR
                     HMSIPVFAVSIITDLGVEGKIEYTTHESVQAAAEHSEEKMTRIMKELIAAL"
     gene            complement(5015..6715)
                     /locus_tag="PKK03_08810"
     CDS             complement(5015..6715)
                     /locus_tag="PKK03_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84541.1"
                     /translation="MKKIILLPLLPLYLSVTAQTGSAAFPFPVTGGIVISEIMADPTP
                     SRGLPEREYLEIFSLSRDTLELKGIMLIAGNDTAYLPEGVAAPGELIILCSTGSRADL
                     LPFGRVMAVKSFPSLNDAGELIALRTPGGSLVHSVSYSPEFLGDGPRSGGGWSAELTD
                     TDNPFHEPDAWSPSTDPSGGTPGRVNSTVTAVADTRCPSVIAAWPQTPGVVAVLFDET
                     IILEESGPWLADGIETMPALSGDPADRTLLVTLAGEIDPGTVLTLQIPPGLTDFAGNQ
                     PCTGDIRTGLPSGPAPGEIMFNELMPDPSPGCSEYMELFNNSGKIFDLSALLLAGSSK
                     GAATAITGLHRQLLPGAFVALTTGREDFASVYPCADEKSVFRADRLPALPDDGGTLVL
                     YDRSLNVIDRVDFSPGMHMIFLSGTEGVALEKVSPGMPSDVASGWHSASGVCGFGTPG
                     APNSVLTGDTGESGGLSLSTGRISPDGDGFEDVISVGVFPGGEGNVITVTVFTDRGYP
                     VRRLAEKVTADAGAQFVWDGVSDSGARLPAGLYMIIAESFNTAGSSRRWKKVCALLYR
                     "
     gene            6796..7788
                     /locus_tag="PKK03_08815"
     CDS             6796..7788
                     /locus_tag="PKK03_08815"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNX84542.1"
                     /translation="MKVAVVGATGMVGRMMLKVLEERQFPVDRLFPAASERSAGKEVI
                     FRGKPAKVVSVMEAVEAVPDVALFSAGASVSKEWAPVFAENGTTVIDNSSAWRMEKGI
                     PLVVPEINAAAITSATRIIANPNCSTIQMLMAIAPLHREYGIRRLVISTYQSVTGTGI
                     RAKTQLENERRGLSGEMAYPYPIDLNCIPQCDVFLDNGYTKEEMKLVNETRKILNDEE
                     IRVTATAVRVPVTGGHSESVNIEFAREFDLDRVRALLASTPGVVLYDDPSQNKYPMPL
                     TAHDHDEVFVGRIRRDFSQEKTLNMWVVADNIRKGAATNAVQIAEYMMSANLIK"
     gene            complement(7781..8968)
                     /gene="hflX"
                     /locus_tag="PKK03_08820"
     CDS             complement(7781..8968)
                     /gene="hflX"
                     /locus_tag="PKK03_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNX84543.1"
                     /translation="MERAVLIGVITPEISEASARDYLDELAFLAETAGAVPVRKFLQR
                     VEHPNPRTFVNKGKIEEIAAFIAAGEITLAIFDDELTPSQIRNIEKDLNCRIIDRTNL
                     ILDIFAGRARTSHARTQVELAQYQYLLPRLTGMWTHLERQRGGIGLRGPGETEIETDR
                     RIIRDKITKLKEQLKKIDTQMNTRRKGREKMVRVALVGYTNVGKSTLMNQISKTDLFA
                     ENKLFATLDTTVRKVVLGNLPFLLSDTVGFIRKLPHDLVESFKSTLDEVREADLLLHV
                     TDISHPGFEEQLAVVNATLRELGSLNKPTILVFNKTDLFTFTRKDEDDLTPLKRENLS
                     LEDLKKTWMAGAADQQTVFISAKKRDNLDELRRMLYAEVKKIHVKRYPYNDFLFESEE
                     PVI"
     gene            complement(9136..10308)
                     /locus_tag="PKK03_08825"
     CDS             complement(9136..10308)
                     /locus_tag="PKK03_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84544.1"
                     /translation="MKRTIGILFVAAMLMSGCGSSKKQLETGNYDAAINKAVAELRKK
                     PDSEKDIETLERAMNIALEQDNERIRFLKIEGRANAWDEIYVIYKKMSDRQAAVRTVT
                     PIRYQGRSIEWPYVDYMQEMVIAKKKAADFYYAHGQELMKNNTKDSYRQAYAEFARAR
                     EYVGDYPNIDQMMQESHYLGISRAWVTVKNYSVIKFPEEFEKELLALDLPRLNSEWVE
                     YYTTIPNNDTQIDYQININLRTIAVSPDRQFQRDTLMKATVEDGFDYVKDTRGNVMKD
                     SLGNDIKVKKFRELQCALIQTVQVKECVIQGDVEMLSLYPERTIKKEPIGARSDFEHI
                     SARAIGDVNALSAEQKKMLQSQPVQFPLDIDMVIMCSENLKTAIRGFMEANRRYIN"
     gene            complement(10380..11066)
                     /locus_tag="PKK03_08830"
     CDS             complement(10380..11066)
                     /locus_tag="PKK03_08830"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNX84545.1"
                     /translation="MEPKKSEKANLENKKSMFIQVGLIVALLVCLGAMEWTSGQKKDS
                     AFAGMTEEAIEEEQIPVTEETPPEEMPPPEVTVTDLFEIVEDDVVIDNEVKFEDDETS
                     EDKVVEIYAPVLQAEEEEVEEEIFVIVEDMPKFKGGDINKFREWVQKRVRYPELAAEN
                     GIQGRVFITFVVETNGNVSNVTISRSVDQLLDEAAKEAVMASPKWEPGMQRGRPVRVR
                     YSIPIIFQLQ"
     gene            11217..11290
                     /locus_tag="PKK03_08835"
     tRNA            11217..11290
                     /locus_tag="PKK03_08835"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11251..11253,aa:Ala,seq:ggc)
BASE COUNT         2815 a         3111 c         3083 g         2775 t
ORIGIN      
        1 gttttgctgc atgtcaatga agatcacaga ctgggctgtc gaggacaggc cccgtgaacg
       61 gctctggaac aagggcccct caagcctcag cgatgctgag ctgttagcca ttctcatagg
      121 atcaggtacc aggaataact cagccctgga cctggcacgc gaactgctgg ccatggccgg
      181 taactcactc ggccagctgg gaagactcag cgccggcgag atacggaaaa tcaaagggat
      241 gggtgaagct aaagcggtca ccatcgctgc agccctcgaa ctgggccgac gacgaaaaat
      301 ggctgaagcc gctgataatc cgcagatacg ctcatcagcc gacgtgttca gcattatcag
      361 ccccctgatg gaagacctct ctcatgagga gttctggatc ctctttctca acagggcaaa
      421 ccgggtgaca ggcaggatga aaatcagcca gggaggcgta agcggtacag tcaccgacgt
      481 acgcattgtc atgaaaaaag cgatcgagac actcgcatcc gggctcgtca tctgccataa
      541 ccacccttcg gggaacacca gcccaagcga ctctgacatc cgcatcaccc agaagattaa
      601 ggaggcaggt gcgctgatgg atatccagct gcttgaccac cttatcgtgg ccgggaagga
      661 ttattacagt tttgctgaca acggggcaat gtgaaaccgg ccggcatccg gctggttcat
      721 aacagcacca ggttggcaac cggcctgatt catccctggt gacggctgat tccgcggcct
      781 tctttataac tgacgggcag ccagcctgac atcggaattg tctgcacaga gctgcagcat
      841 ctcagccacc gtcagcccta tgaccggatg aacagctgca ggggccacct ccgagagcgg
      901 ttccaggacg aacctcctgt cagctatctt cggatgagga acctcaagcc cgggaatgga
      961 aatgaccctt tcctcccata agagaatgtc aaggtcaatg atacgcgact catatttacc
     1021 tccgccgctg cgcttccgcc ccatgcgccc ctcgattgac ctgaaaagct gcatgagctg
     1081 tgccggcctg cggctggtct ttatcacagc aaccatgttc agaaactttt catcggcatc
     1141 aaaaccccat ggctcggtct cccagacaga ggagagcgca gtgatctctc ccgcctcctc
     1201 cccgagcagc tccagcgccc gctgaagggc tgcatacctg tctcccaggt tgcttccgag
     1261 gcccgcgatg acctctgaac aggctgataa tttcatgact tgtagttcag gcctttaaag
     1321 atgcctgttt tttttataat ttggtcaatt aattcaatag tacagagatg aaaaccttcc
     1381 ttaaaatggt cctggctgtt atagcaggaa taattatcac cagcatcctc ttttttgtgg
     1441 tcatgctctc ggtcttctcc gggatggcag cggcaggaaa gaaatcggtg gtaatccccg
     1501 aaaaatcagt gctcgtaata aagaccggag ctatgatccc tgaccgctca cagagcaatc
     1561 cgctggcaat gcttgacccg gtgacaatgt cctttacaca gaccccgggc ctcaacgagt
     1621 tgctcagtaa cctgaagaag gcagctgaag atgagaaggt gagcggcatt ctgatcgaga
     1681 acggaaccat gccctcggga tgggcaacag ctgacgagct gcgcagcgca ctgcttgaat
     1741 ttaaaaagag cgggaaattt atctattcct attcagatta tttgctgatg caggagagct
     1801 atttcatctc aacagctgca gacgccatct ggatcaaccc cacctcaatg ttcgacttca
     1861 aggggctggc cgccgaagtc accttttata aggatgccct ggaaaagctg ggcgttgatg
     1921 tccaggtaat aagacacggc aaattcaagg gggccgtgga accttatatg ctcgacaggc
     1981 tgagcgacga gaaccgcctc cagatatccg ggtacatggg ctcgatatgg gaacatgtgg
     2041 tgaagtcggt ttctgagtcc cgcggcgtac ccgccgaaaa gataatgagc ctcgccgatt
     2101 cacttgcggg atatgacgct gcacttatga ccgccgccgg gctgattgac ggcaccattt
     2161 atcgtgatca gctcgaagac agtatcagga ctgcagcagg gattgacagt ggcaagaaga
     2221 taaattatgt ctcaatgtca aagtattcaa acgttaccgt gaagcacagc accgtaacag
     2281 ggaaagacaa gatagcggtt ctcttcgccg agggcagcat agtcatgggt gaaggcgatg
     2341 atacaaacat cggcggcaac aggtatgcgg aggagcttcg caagctccgg ctagactcca
     2401 ctgtaaaggc aatcgtcttc cgggtcaatt cgcgcggcgg caatgccata gcctccgacc
     2461 agatatggcg tgaagtggag ctgactgaca aagtcaagcc tgttgtggtc tccatgggca
     2521 actatgccgc cagcggaggt tactatatcg ctgccgccgg gcggaagata attgcatcac
     2581 ccgttaccat aaccggatcg ataggcgtct tcggccttat ccccaacgca cagacactcc
     2641 ttgacaagaa gctgggaata agctccgatg tggtgcgcac aaacgcacac tctgactcac
     2701 cttcgctgac ccgtccgctc agtgcctttg aaaaggatgc ccttcaggac aatgtggaac
     2761 ggacatacaa caccttcacc ggtgtggttg ccgcaggccg cgagatgagc cagcctgcag
     2821 ttgacagcat aggacagggc cacgtctgga gcggcgccga cgcaattccc atcggccttg
     2881 tcgactcgtt cggcgggctc agggacgcaa tcagtgaagc tgccaccctt gccgggctgg
     2941 aggaatacag aactatagaa aagccggaga gcgcagacct ttatacaaag ctgctgaagg
     3001 aacttgccgg cggtatcagg gcgaaggcca tccgccagga actgggagaa gtctcaaggt
     3061 attacttcga cctgaaggac atcatctcct ccggaggaat acaggctgca atgccatact
     3121 acctggaaat ccgctaggat gataatgccg gggaagcttt tcctgtgggc cgtttcaaag
     3181 atctacggca tggccgtggc agtgaggaac aggctctatg acctcggtat cttccggagc
     3241 cattcattca gcgttccggt tatctgcgtg gggaatatca cagccggggg tacgggcaag
     3301 acaccgcatg tgatttacct ggctgaaaaa ctttcatcac cgggctctgt ggccgtgctc
     3361 agccgcgggt acctgcgcag gagccgcgga ttcatgaggg tcaccgctga cagtaccacc
     3421 gccgcctcag gagacgagcc gctgctgatg gcacggagcc ttcctcaggc acaggtcttc
     3481 gttgaccgcg acagggtaaa cggcatcagg gaaatcatgc tgacagaccc cggtaccagg
     3541 gttgtgatcc ttgatgacgg ctttcagcac agggcagtca gagccggaat gaatatcata
     3601 cttaccgact tcaacaggct gatgacacgt gaccgtctgc tgcccctggg gatgctgcgc
     3661 gagcatataa aagccgtcag aagggccgat gcggtaattg tgaccaaaac tcctgaagtg
     3721 acctcaccat ctgtcaggga ggagatcagg agagagctga ttgcagcagg tccatgcgag
     3781 accattttct ttacctccct cgcttacggg cggccccttc acctttacag cgaaaacgca
     3841 ggcgcgatcc ggcgaaccac atccgtgctc cttgtgacag gaatagccaa cccggcacca
     3901 ctggaagaat acctcgggag catcgcaggt cacgttaacc atcttgcctt ccccgatcac
     3961 cacgcttaca acaccgcaga tatcggcaga ataacctctg ccttcaatgc aatgagcggg
     4021 ccagacaaaa taatcgtaac cactgccaag gatggcgtca ggttaaaaga aatcactaat
     4081 attgcagacc atgtcaggca ggcgttacat tacctgcccg tcagggtgca attcattgaa
     4141 gatgaagaac tatttctaaa taaagtttac tcatatgctg gaaaagatta tcaaaaccgc
     4201 tgaattcctg gcttcaaaag ggattaagga tcctgatgcc ggcattgttt taggaacagg
     4261 actgggcggt ctgacggcag agattgacat ccaggttgaa atcccctaca aggatattcc
     4321 cgattttccc gtttcaacgg ttcagggcca tgccggcaag ctcatctatg gcgatttcgg
     4381 tggcaagaag gtggttgcca tgaagggacg ctttcattat tacgagggat acggctccga
     4441 acaggttaca ttccccatca gggtcatgaa ggaactgggc atcagggcgc tgttcctctc
     4501 caatgcagca ggtggagtca acccgaactt caggattggc gatatcatga taatagacga
     4561 tcatatatgc cttgttgaca atccgttgat cggccccaat gatgaccgca tcggtccccg
     4621 cttcccggat atgagtgaac cctatgacaa aaagctcatt gagaaggcaa aggcgattgc
     4681 cacaggactg aatatacctg tcaccagtgg agtatatctc tcgaccacag gcccgacttt
     4741 tgagactcct gccgaataca ggttcttccg gatcatcggg gcagacgcag ttggtatgtc
     4801 aacagtgccg gaggttatca ctgccaggca tatgagcatc ccggtgtttg ccgtcagcat
     4861 aatcacagac ctgggcgttg aagggaagat cgagtacacc actcatgaat cggtccaggc
     4921 agcggcagaa cacagcgaag agaagatgac aaggatcatg aaagagctca tcgcagctct
     4981 ctgatctgca aatatgtcat tgattgtatt actgttacct gtaaagcagg gcacatacct
     5041 ttttccacct gcgcgatgag ccggcagtat tgaatgactc agcaattatc atgtataatc
     5101 ctgccggcag cctggcaccg ctgtccgaca ccccgtccca gacgaactgt gctcctgcat
     5161 cagcagtgac cttctcagcc agccgcctta ccggataacc ccggtcagtg aataccgtca
     5221 cagtaatgac attcccctcc cctcccggga aaacacccac cgagatcaca tcctcaaatc
     5281 cgtcgccgtc aggcgaaatc ctgcctgtag agagggagag accgcctgat tcacctgtat
     5341 ctccggttaa aactgaattt ggcgctcccg gagtgccgaa accacatacg ccggaggctg
     5401 aatgccagcc ggacgcaaca tcagatggca tgccgggtga gactttctct agcgccaccc
     5461 cttcggttcc tgaaagaaag atcatatgca tgccgggact gaagtcgacc cggtcgatga
     5521 cgttgaggct cctgtcatag agcaccaggg tgccgccgtc atcaggcagc gcaggcagcc
     5581 gatcagcccg gaacactgac ttttcatcgg cacaggggta gaccgaagcg aaatcctccc
     5641 tacctgtagt gagagccaca aaagctccgg gaagaagctg acgatgcagg ccggtaatgg
     5701 ccgttgccgc ccccttgctg cttccggcaa gcaacagggc ggacaggtca aatatcttcc
     5761 ccgaattgtt gaacagctcc atgtattcac tgcatccggg ggaagggtca ggcattagct
     5821 cattgaacat gatctcaccc ggcgcaggac cagaaggaag acctgttctg atatcgcctg
     5881 tacagggctg attcccggca aagtcggtca gccccggggg gatctgcagg gtcagaacag
     5941 tacccgggtc aatctcccct gccagggtca caagaagggt gcggtcagcg ggatcgcctg
     6001 ataatgcagg catggtctca atgccgtcgg ccagccatgg cccggattcc tccaggataa
     6061 ttgtttcgtc aaaaagcact gccaccaccc cgggcgtttg aggccaggca gcgattaccg
     6121 aagggcatct cgtgtctgca actgccgtga cggttgaatt tacccttccc ggtgtgcctc
     6181 ctgatggatc cgtcgaggga ctccaggcat caggctcgtg gaacggattg tcagtgtcgg
     6241 tgagctctgc tgaccagcct ccgcctgagc ggggtccgtc acccaggaac tccgggctgt
     6301 agctgaccga atgtaccagg gagccgccag gggtacgcag ggctatcagc tccccggcgt
     6361 cattcagcga agggaacgat ttcacagcca tgacccttcc aaacggaaga aggtcagccc
     6421 tgcttccggt ggaacacagt atgatcagct ctccgggagc ggccacacct tcaggaagat
     6481 acgcagtgtc attccctgcg atgagcatga tccccttaag ttcgagggta tccctgctca
     6541 ggctgaatat ctcgagatac tcgcgttcag gcaggcctcg tgacggggtg ggatcggcca
     6601 ttatctcgga aatgacaatg ccgcctgtga ccgggaaagg aaaggcagca ctgccggtct
     6661 gtgccgtaac agacagatac aatggcaata gcggcaacag tataatcttt ttcatgcagc
     6721 gggactaaaa aacgattaaa tttgtcgcac ttttctcttt ataaagttcg tacaattttt
     6781 ttaaaaacga aagaaatgaa agtagcagtt gtcggtgcaa ccgggatggt tggcagaatg
     6841 atgctgaaag ttctcgaaga gagacaattt ccggttgaca ggctttttcc ggcagcatcg
     6901 gagcgttcag ccggcaagga ggtgatattc aggggcaaac cggcaaaggt ggttagcgtg
     6961 atggaagccg ttgaggctgt tcctgacgtg gcgctgttct ccgccggtgc atcggtttca
     7021 aaggagtggg ctcctgtctt cgccgaaaat ggcaccacgg taattgacaa ctcttccgca
     7081 tggagaatgg agaagggtat accgctggtg gtacccgaaa taaacgctgc tgcaatcact
     7141 tcagcaaccc gcattattgc caatcccaat tgctctacca tacagatgct gatggccatc
     7201 gctccccttc acagggagta cggcataagg cggcttgtga tctcaaccta ccagtcagtg
     7261 accggcaccg gcatcagggc caagacacag cttgaaaacg agagacgcgg gctgagcggt
     7321 gaaatggcat atccctaccc catcgacctt aactgtatac cacagtgcga cgtcttcctt
     7381 gacaacggct acacgaagga ggagatgaag ctggtgaacg aaacacgcaa gatactgaac
     7441 gatgaagaga tacgggtcac cgccacggcc gtaagagtcc cggtgacggg agggcattca
     7501 gagtctgtaa acattgagtt tgcccgcgaa ttcgaccttg acagggtgag agcgctgctt
     7561 gcctcaaccc ccggtgtggt gctgtatgat gatccctcac agaacaagta ccccatgccg
     7621 ctcacggctc atgaccatga cgaggtgttt gtgggacgca tcaggcgcga tttctcgcag
     7681 gagaagaccc tcaacatgtg ggttgtggct gataacatac gcaagggcgc tgcaaccaac
     7741 gcggttcaga tagctgaata catgatgtct gcgaacctga ttaaatgaca ggctcctcag
     7801 actcaaacag gaaatcattg taggggtaac gctttacgtg aatctttttt acctccgcgt
     7861 acagcatccg cctcagctca tcaagattat cacgcttttt ggctgagatg aatactgtct
     7921 gctggtcagc agcacccgcc atccatgtct tcttcaggtc ttcgagcgac aggttctcgc
     7981 gcttcaacgg ggtaagatca tcttcatcct tccgggtgaa agtgaacagg tctgtcttgt
     8041 tgaagacaag aatagtcggc ttgttgagcg accccagttc ccggagggtg gcattcacca
     8101 cagccaactg ctcttcgaaa ccgggatgag aaatatcagt gacatgaagc agcaggtcgg
     8161 cctccctgac ctcgtcgagg gttgacttga acgactccac caggtcatgc ggcagcttac
     8221 ggatgaaacc caccgtgtca gaaagcagga atggcaggtt gcccagcacc accttcctca
     8281 ctgtggtgtc aagagtggca aagagcttgt tttccgcaaa caggtcagtc ttgctgatct
     8341 ggttcatcag ggtcgatttc ccgacgttgg tataaccgac aagggcaacc ctgaccattt
     8401 tttccctccc cttgcggcgg gtgttcatct gggtgtcgat cttcttgagc tgctccttga
     8461 gcttcgtgat cttgtcacgg atgatacggc ggtcggtctc aatctcagtc tcacccggtc
     8521 ctcgcaatcc gatacctccc cgctgccgtt cgaggtgagt ccacatgccg gtgagtctcg
     8581 ggagaaggta ctggtactga gccagctcca cctgggttcg ggcatgcgag gtacgggccc
     8641 ttcccgcaaa aatgtcaagt atgaggttgg tgcggtcgat gatgcggcag ttgagatcct
     8701 tttcaatatt gcgtatctgg cttggggtga gctcatcgtc aaagattgcg agtgtgatct
     8761 caccggcagc aataaaggca gctatctcct ctatcttccc cttgttgaca aaggttcttg
     8821 gattgggatg ttcaacgcgc tgcaggaact tcctgaccgg cacggctccg gccgtctctg
     8881 ccaggaacgc aagctcgtca aggtaatcgc gggcgctggc ctcgctgatc tccggggtga
     8941 tgacccctat caataccgct ctttctattt ttcagaaggt tattcagtta gtattattcc
     9001 ctgctcttcc ggcttatgca gccggtaaga gctcccctga ctcagtagcc ttagggcggc
     9061 tgtcataccg taacaaaagt gacggaaaga aaccgccccg gtctttcagg gccggggcgt
     9121 caaaaataat acaatctagt taatatacct cctgttggcc tccatgaagc ccctgatggc
     9181 agtcttaaga ttttcgctgc acataatgac catgtctata tccagaggga actgaaccgg
     9241 ctgtgactgc agcattttct tctgctccgc tgacagtgca tttacgtcgc ctattgccct
     9301 ggctgagata tgttcaaaat ctgaccttgc tccgataggc tcctttttaa tggtacgctc
     9361 gggatagaga gaaagcatct caacatcgcc ctgtatgaca cattccttga cctgtaccgt
     9421 ctggatgagg gcgcactgaa gctccctgaa cttctttacc ttgatgtcat tgccaagcga
     9481 atccttcatc acgttgcccc tggtgtcctt gacatagtca aagccgtcct caaccgttgc
     9541 cttcataagg gtatcgcgct ggaactgcct gtcgggactg acagctatgg tgcgaaggtt
     9601 gatattgatc tggtaatcga tctgggtatc attgttcggt atggtggtgt agtattccac
     9661 ccattcgctg tttagccgtg gcaggtcgag ggccagcagt tccttctcga actcctcggg
     9721 aaatttaatg acagaatagt ttttgactgt tacccaggcc cggctgatgc cgaggtaatg
     9781 tgattcctgc atcatctggt caatattcgg gtaatcaccc acatattccc tggcgcgggc
     9841 aaattcggcg tatgcctggc ggtatgagtc cttggtgttg tttttcatca gttcctggcc
     9901 gtgagcgtag tagaagtccg ctgccttctt ctttgcgatg accatctcct gcatgtaatc
     9961 aacgtaaggc cattcgatgc ttctcccctg gtacctgata ggggttacgg tacgaacagc
    10021 tgcctggcgg tcgctcatct ttttatagat cacatagatc tcatcccagg cgttggctct
    10081 gccttcgatc ttcaggaaac gaatccgttc gttgtcctgt tcgagggcga tgttcattgc
    10141 ccgttcgagg gtttcgatgt ccttctcgct gtcgggcttt tttctgagtt cagccacggc
    10201 tttgttaata gccgcgtcat agttgccggt ttcaagctgt ttttttgacg atccgcagcc
    10261 tgacatgagc atggctgcaa caaataagat tccgatggtc cgtttcataa caatggattt
    10321 aaaaaacccc gctgttctta ttgaaggcgg ggtttatatt taacgctggt tacgtcagat
    10381 tattgcagct ggaatatgat ggggattgaa tatctcaccc tcaccggccg gccgcgctgc
    10441 atgccgggtt cccatttggg tgatgccatc actgcttcct tggcggcttc gtcaaggagc
    10501 tggtcgacgc tgcgggagat tgtaacgttg ctgacgttgc cgtttgtctc aaccacgaag
    10561 gtgatgaata ctcttccctg gattccgttt tcggctgcca gttcggggta gcggacccgt
    10621 ttctggaccc attcgcggaa cttgttaatg tcaccgccct tgaatttggg catatcttca
    10681 acgatgacga atatttcctc ttccacctct tcctcctctg cctggaggac aggggcgtaa
    10741 atttcaacca ctttgtcttc ggatgtttca tcatcctcga atttcacctc attgtcaatg
    10801 accacgtcgt cttcgacgat ttcgaagagg tcagtgacag tcacttcagg cgggggcatc
    10861 tcttcggggg gagtttcttc cgttacgggg atctgttcct cctcgattgc ctcctcggtc
    10921 attccggcga aggccgaatc ttttttctgg ccgctggtcc attccattgc accgaggcag
    10981 acaagaaggg caactataag gcctacctgg atgaacatgg atttcttgtt ctcgaggttt
    11041 gccttttctg atttcttcgg ttccattgta atgttgttat aggcagttca aagttacaaa
    11101 attgtttgtg atctccgact gtgggttatg aagtgatttc gaaaaaattt ctatatttgc
    11161 accgctccgg gcgtttggag ttttttgtcc ggcaccgggg ttgttcattt aagcatgggg
    11221 atatagctca gttggctaga gcgtgtgact ggcagtcaca aggtcagggg ttcgattccc
    11281 cttatctcca caaattcaat tagttacaaa attggagtcc tgtaaatcaa atgtttgcag
    11341 gattcttttt ttcatccatt cccttcatct acaccgcaat atacctacct gtgcggctta
    11401 ttcggtggca attttcaaat cttgaatttg tccaccgaat aagctgtaat gtactggtat
    11461 tcttcggctt acaattcagt aatttgtaat tatcaaccag taatatcagt atcaccggaa
    11521 gaagaacctt ttccttaatg ttctttatca gaagaacgaa gttgcacaag aacttcgaag
    11581 caccggtaat gttgagaatt acagtgaacg gtgacaggac ataaccagta aagagcaaat
    11641 cagaatgacc ttgactttat agcaggcagg aaatattttt gactggcaat atgatgatta
    11701 tttcatttta ggattgcaac atgaccatct gtaaacggca aataacaatg tacgtttttt
    11761 cgcaaataag aatgtactac aatt
//
LOCUS       DAOPXU010000083        11736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_6212_length_11736_cov_11.325828, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000083 DAOPXU010000000
VERSION     DAOPXU010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11736
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6212_length_11736_cov_11.325828"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="PKK03_08840"
     CDS             complement(<1..1208)
                     /locus_tag="PKK03_08840"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HNX84546.1"
                     /translation="MVKSIPRIKLLSLSIFFLSCPLFSFSSPEFSSVESADENVKSVC
                     FEKEGWKLAYPFLQPVSGDEMTLSFDLIDGNAGTLWYNITHCDRDWNKSDLFTSDYLE
                     GYDENQVTDFLPSFNTKVNYVHYRLTLPNSDVRFLVSGNYVITVWEASEPDKPLLVRR
                     FYISEGSTSAAVVFRRPMKPGTTDTHQQAEITVTTGGLPVTDPYRQVTLTVMQNGRAD
                     RMHAGLTPDFVGNGRLEYNTLSDKTLFPGGNEFRYFDIKTVRQTRQNVRAIDFVNGVY
                     NAFLIPSDNREFKPYFFNEDFNGKSVVAIEESNDPDREADYLWVWFTLPAYQEQRGGS
                     VYVTGDFCSWQYTSDNRMSWNTAKGCYEASLLLKQGWYNYEFAFLPDGASSPEGFHFE
                     GSHWETENDYL"
     gene            1362..2714
                     /locus_tag="PKK03_08845"
     CDS             1362..2714
                     /locus_tag="PKK03_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HNX84547.1"
                     /translation="MSTLIRLRKGLNIRLAGKAEKILLPEVAVTRFGVRFADFPGLVA
                     RLEVQEGDKVMCGSVLFHDKAFPEIKYVSPVSGVVREIIRGEKRLLTEVVIGQEGSEK
                     VDFGSAAPLSLTREQVKEKLLSSGMWPVLRQRPYHIVARPGIVPRDIFISTFDTAPLA
                     PDLDFVMANTHGGYLQTGIDALSRLTDGKVHLSQDAEGTRVAEMKNLKNVEFHRFSGP
                     HPAGNVGVQIHHIAPVSKGEVVWTLDLQDVIAIGRLFTTGFHDRERIIALAGQGAQHR
                     KYHRIKPGASVAGILKGNLVEGNLRVISGNVLTGKKISPEGSFGFYDNMVTVIPEGDY
                     HELLGWAAPGLKKFSYWRTFISKLLPQREYNLDTNLHGGHRAFVVTGHYENVLPMDIY
                     PVHLLKAILAGEIELMENLGIYEVAEEDFALCEYIDPSKTEIQEIIRSGINLMIKEMN
                     "
     gene            2733..3890
                     /locus_tag="PKK03_08850"
     CDS             2733..3890
                     /locus_tag="PKK03_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HNX84548.1"
                     /translation="MSFLLDKMKKIEPLFEKGGRFEKLHSVYDGFATFLFVPDKVTTR
                     GSHIRDHMDMKRTMTVVIIALMPALLFGSYNIGLQHTRAFGINDWTLMNMFWFGFLKI
                     LPLIIVSYVVGLGIEFIAAQIRGHEINEGYLVTGMLIPLIMPVDVPLWMLAVSVAFAV
                     IIGKEIFGGTGMNILNPALTARAFLFFSYPGWMTGDAVWIEGLTKGGGLDGFSGATPL
                     ALAKLDGFSQMPSAMEMFIGTIPGSVGETSTVAILIGAFILLLTGVGSWRIMLSMTLG
                     ALGMGLLLNATGGNEYTQIPAYYHLIMGGFAFGAVFMATDPVTAAQTGTGKYIYGALT
                     GILVVIIRVFNPAYPEGTMLAILLMNVMAPLIDHYVVQANIRRRIKRVKTA"
     gene            3902..4603
                     /gene="nqrC"
                     /locus_tag="PKK03_08855"
     CDS             3902..4603
                     /gene="nqrC"
                     /locus_tag="PKK03_08855"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HNX84549.1"
                     /translation="MKDFSNKYIIIFSVVMVVSVAILLSLMALLLQPRQEKNVEVEKK
                     MNILSSINVVSDKSDAVQLYDRHIKESFVINTEGKKFEGVDAFTVTLRAEQKKPLAEQ
                     YLPVFIAVADNGDTLHIIPLEGKGLWGPIWGYVSLKKDLSTIAGATFDHKGETPGLGA
                     EINTTPFESQFHDKSIYENGNFVSVRVVKGGARPGNLHEVDAISGGTITSKGLEAMVH
                     DGLEKYEKYLSQNRK"
     gene            4606..5259
                     /locus_tag="PKK03_08860"
     CDS             4606..5259
                     /locus_tag="PKK03_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HNX84550.1"
                     /translation="MEKVEKEPLLSAKNRKLLTNPLGIDNPITVQVLGICSALAVTVK
                     MKPAIVMAISVTVVVALSNLIIALMRKSIPGRIRIIVQLVVIATLVIIVDQFLKAFAF
                     DVSKQLSVFVGLIITNCIIMGRLEAFAMANKPWPSFLDGIGNGAGYGLILIIVSFFRE
                     FLGSGSIFGYPVMEKLGVYKFGYEDNGMMILPPMALITVGLIIWFQRARNKSLIENK"
     gene            5270..5887
                     /gene="nqrE"
                     /locus_tag="PKK03_08865"
     CDS             5270..5887
                     /gene="nqrE"
                     /locus_tag="PKK03_08865"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HNX84551.1"
                     /translation="MENLVNIFVKSIFIDNMIFAYFLGMCSYLAVSKTVKTSMGLGIA
                     VTFVLLITVPVNFLLEQYVLKKGALVWLDESFATLDLSFLSFIMFIAVIASIVQLVEM
                     VIEKSSPALYNSLGIFLPLIAVNCAILGASLFMQEREYENIAEATVFGLGSGIGWLLA
                     IVALAAIREKMRYSNVPAPLRGLGITFILTGLMGIAFMSFLGITL"
     gene            5903..7159
                     /gene="nqrF"
                     /locus_tag="PKK03_08870"
     CDS             5903..7159
                     /gene="nqrF"
                     /locus_tag="PKK03_08870"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HNX84552.1"
                     /translation="MILGLSIAGTVLISIAVFVLVMILLVVVLLYARQKLTPQGDVTL
                     KINDREMVVSPGSSLLSTMSNNGIFLPSACGGGGTCGMCRCQITEGGGSILPTETGFF
                     TRREQHDNWRLGCQVKVRESMTIKVPEAVLGVKKWECEVVSNRNVATFIKEFVVKLPE
                     GEDLNFRSGDYIQIDVPKYDVEFGRDIDVDEEYRADWDKFKMWDLKHKNTEETYRAYS
                     MANFPAEKNLIILNIRIATPPFDNARKQFMNVPPGICSTFIFSRKPGDKVSISGPYGE
                     FHIKHTDNEMVYVGGGAGMAPLRSHIFHMLKTEKSGRKISYWYGARSKREIFYEDEFR
                     ELEKKFPNFTFNIALSEPLPEDNWTGYKGFIHKVLLDNYLSRHKLPEEIEYYLCGPPV
                     MNAAVLNMLDNLGVPEENIAFDDFGG"
     gene            7253..8257
                     /locus_tag="PKK03_08875"
     CDS             7253..8257
                     /locus_tag="PKK03_08875"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNX84553.1"
                     /translation="MRITLFCSAIALMMASCSPQPEYAELNGFTQGTTYHIVVEKIPG
                     LDVMALRQDIENLFTGIDNSLSIYNDSSVISAINFNRSDLTDTLFREVFRASEVISAE
                     SGGLFDITIGPLVKSWGFGPDAMKRFNPSMLDSLMSLVGMNKVRLEGDRIIKTDPGMF
                     IDVNAIAQGYTVDLAADLIVRMGIKQCLVEVGGEVRTAGDKNGMGWKVGIDAPADGNF
                     TPGADIQATIRLDDRALATSGNYRKFYVENGVKYSHTIDPRTGYPVRHTLLSATIIAP
                     TGAVADAWATACMVGGKDAAIAFIEKYDFLEGYLIYSDETGAMKSWVSEGIRKMILTE
                     "
     gene            complement(8365..8589)
                     /locus_tag="PKK03_08880"
     CDS             complement(8365..8589)
                     /locus_tag="PKK03_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84554.1"
                     /translation="MLLKLLIISAILVGLSMAGLAISILLKPKGQFPDTHVGHNKEMR
                     KRGITCAKSTDTGCNPIDSEGCCGCAVNSD"
     assembly_gap    8730..8829
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9134..10645)
                     /gene="nhaC"
                     /locus_tag="PKK03_08885"
     CDS             complement(9134..10645)
                     /gene="nhaC"
                     /locus_tag="PKK03_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HNX84555.1"
                     /translation="MSLKQYKTPTMLQSLLPIAVLIVFLTLNVKFFRDDTLSGANQMA
                     LILAAAVAGIIAVNLGYRWLFIRERVIKGIGTAMPAILILFLIGSLAGTWMVSGVVPM
                     LIYYGLEILHPKIFLFASVIICAVVSVSTGSSWSTVATIGVALLGIGKALGFSEAMIA
                     GAIISGAYFGDKMSPLSDTTNLAPAVAGTDLFTHVRYMVITTTPSLIITMVIFLIIGF
                     TIDLQGGATNADAVQAAIKAKFNVTPVLLLVPAMLIYIIVKKVPPLPSLLAGTLLAAV
                     FAIIFQPHILEEVAATGGSFVKQAYIAIMKTMFGSVAVTTGDPSVDELMKTRGMAGML
                     NTVWLIICAMVFGGIMEACGMLKTITGKLMKFVHNAGTLVGSTVATCLFFNVTASDQY
                     LSIVIPGRMYAKKFRDMGFKPELLSRSLEDSGTVTSVLIPWNTCGATQSGVLGVSTWA
                     YAPYCFFNIISPFMSVFIAAIGYKIRKYTPEEHAKVLEEADAADRELYREEEV"
     gene            10748..11335
                     /locus_tag="PKK03_08890"
     CDS             10748..11335
                     /locus_tag="PKK03_08890"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNX84556.1"
                     /translation="MPQYPDIFSINPEDKIPGKGRVLISEPFLTDRFFNRTIVFLTEH
                     TPEGTVGFILNRVTDLMVSSAVEGFDGWDEHLSVGGPVSPDTLHYLHSLGTKVPGSVP
                     VIPGIWWGGEIDALRNLIATGTVGRDQVRFFLGYSGWEKGQLKRELKENSWVIAKVPP
                     EVIMKSRGNVWRDVLRSLNNKYRIWAEFPESPEMN"
     gene            complement(11414..>11736)
                     /locus_tag="PKK03_08895"
     CDS             complement(11414..>11736)
                     /locus_tag="PKK03_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNX84557.1"
                     /translation="RVVNYYAGHSKKEIIPLIFPVLFSSFSRVLIYHSLKDKSDGNVV
                     AMLKTYPGYVRDFKAAAALYSVGACMNIISLVRSYDLKAKGFGEAGSDAGDLLRELVF
                     KIMH"
BASE COUNT         2840 a         2932 c         3089 g         2775 t
ORIGIN      
        1 aggtaatcat tctccgtctc ccagtggctg ccttcgaaat ggaatccctc aggcgatgat
       61 gccccgtcag gcaaaaacgc aaactcataa ttgtaccatc cctgtttcag cagcagcgaa
      121 gcctcgtagc atcctttggc ggtgttccac gacatccggt tatcagatgt gtactgccat
      181 gagcagaaat cacccgtcac gtagactgac cctccccgct gttcctggta tgccggcagc
      241 gtgaaccaga cccacaggta atcagcctcc cggtcgggat cattgctctc ctcgattgca
      301 acaaccgatt tgccgttgaa gtcctcattg aagaaatacg gcttgaattc cctgttgtca
      361 gaaggtatga gaaacgcatt gtacactcca tttacaaagt caatggccct gacattctgc
      421 ctggtctgcc tgaccgtttt aatgtcaaag tacctgaact cattaccgcc agggaataaa
      481 gtcttgtctg agagggtgtt gtattccagc cttccgtttc ctacgaaatc aggggtgagg
      541 ccggcatgca ttctgtcagc ccggccgttt tgcatcacgg tcagggtcac ctgccggtac
      601 gggtcagtga cggggaggcc acccgtggtg acggtgatct cagcctgctg gtgcgtgtcg
      661 gttgtgccgg gtttcatcgg cctgcggaag accacggcag ctgaggtgct cccctcactg
      721 atataaaaac gcctgacaag cagcggcttg tctggttcgc ttgcttccca gactgtgatg
      781 acatagttgc ctgataccag gaaacggaca tcgctgttcg ggagggtgag acggtagtgg
      841 acgtagttca ctttcgtgtt gaacgaaggg aggaagtcag tcacctggtt ctcatcatat
      901 ccctcaaggt agtcacttgt aaagaggtca ctcttgttcc agtcacggtc acaatgtgtt
      961 atgttatacc aaagggttcc ggcgttgccg tcgatgagat cgaagctgag agtcatctca
     1021 tcgcctgata ccggctgcag gaaagggtat gcgagcttcc agccctcttt ttcaaagcag
     1081 acacttttta cattctcatc agcagattcg accgatgaaa attccggaga tgaaaaagaa
     1141 aagagagggc atgatagaaa gaatatcgat aaacttaata attttatccg cgggatagat
     1201 tttaccatta tcactatttt aggactaaat tacaaatatc ctgccgcact ctgaacaacc
     1261 gtctgcaaaa agagttatgt gataaaaatc aacttttctg tgaaaatatt aacaatgtag
     1321 tataattgcc atctctaaag gaattaagat tcaattcaaa tatgtcaact ctgatcagat
     1381 tacgtaaagg attaaacatc aggcttgccg gaaaggcgga gaagattctt ctccccgagg
     1441 tggccgtgac ccgtttcggg gtcaggtttg ccgatttccc gggtcttgtt gcccgtctcg
     1501 aggtacagga gggagacaaa gtcatgtgcg gcagtgtgct gtttcatgac aaggcatttc
     1561 ctgagataaa gtatgtgtca ccggtaagcg gtgtggtccg ggagatcatt cgcggtgaga
     1621 aacgactgct taccgaggtg gttatcgggc aggagggatc ggaaaaggtc gatttcggca
     1681 gtgccgcccc tctcagcctt acccgggaac aggttaaaga gaagcttctg agctccggga
     1741 tgtggcctgt tctgaggcag cgcccatatc atatcgtggc caggcccggg attgttccga
     1801 gggacatttt catctcgacc tttgacaccg ccccgcttgc tcctgatctc gactttgtga
     1861 tggccaatac tcatggcggg tacctgcaga ccggtataga tgccctctca cgtctcactg
     1921 acggtaaggt gcatctttcg caggatgccg aaggaacaag ggtggcagag atgaaaaacc
     1981 ttaagaacgt ggaattccac cgtttctccg gtccgcatcc ggcaggcaat gtgggtgtgc
     2041 agatacatca tattgccccc gtttccaagg gggaggttgt ctggacgctt gacctgcagg
     2101 atgtcattgc tattggaagg cttttcacca caggattcca tgaccgtgag aggataattg
     2161 cgcttgccgg tcagggggcg cagcaccgca agtatcaccg cataaagccc ggggcctccg
     2221 tagccggaat cctcaaaggt aaccttgttg aaggcaacct cagggtcatc agcggcaacg
     2281 tgcttaccgg gaaaaaaatc tcaccggaag ggtcatttgg tttctatgac aacatggtca
     2341 ccgtgattcc cgagggtgac tatcatgagc tcctgggctg ggccgctccg ggactgaaga
     2401 agttcagtta ctggagaaca tttatttcga agctcctccc gcagagagag tataaccttg
     2461 acaccaacct tcacggcggc cacagggcat ttgttgtaac ggggcattat gagaatgtcc
     2521 ttcccatgga catatatccc gtccatcttc tcaaagccat tcttgccggc gagatagaac
     2581 ttatggagaa cctgggcatt tacgaagtgg ctgaggagga cttcgccctg tgcgaataca
     2641 tcgatccttc aaaaaccgag atacaggaga tcatccgcag cgggatcaac ctgatgatta
     2701 aagagatgaa ttaattaagg aaagagcttg ctatgagttt cctgctagat aagatgaaaa
     2761 agatagagcc tctctttgaa aagggaggcc gctttgagaa gctgcattcc gtctatgacg
     2821 gctttgctac cttccttttt gttcctgata aggttacaac gcgcggatcg catatacggg
     2881 atcatatgga catgaagcgg acgatgaccg ttgtgatcat tgcactgatg cctgccctgc
     2941 tgttcggcag ctacaacata ggcctgcagc acacaagggc gttcggcatc aatgactgga
     3001 ccctgatgaa catgttctgg ttcggattcc tgaagatcct gccgctgatc atcgtcagct
     3061 acgttgtggg gctggggata gaatttattg ccgcacagat aaggggtcat gagatcaatg
     3121 agggctacct cgtcaccggg atgctgatcc ctctgataat gccggttgat gttcctctct
     3181 ggatgctggc cgtctcggtt gcatttgccg tgataatagg caaggagatc ttcggaggga
     3241 ccgggatgaa catccttaac cccgcgctga cggcaagagc cttcctcttc ttcagctatc
     3301 ccggctggat gaccggtgat gcggtatgga ttgagggatt gacgaagggg ggaggtcttg
     3361 acggcttttc gggagccaca cccctggcgc tggccaagct tgacggtttt tcacagatgc
     3421 cttcggcgat ggagatgttt atcggtacca tcccgggatc ggtaggtgag acttcgaccg
     3481 ttgcgatact gattggcgca ttcattcttc tgctgaccgg tgttggcagc tggcggatca
     3541 tgctgagcat gaccctcggg gcactcggga tgggactgct gctcaatgcc acgggtggaa
     3601 atgagtatac gcagatacct gcatactacc acctgataat gggtggcttc gcctttggtg
     3661 cggtctttat ggcaaccgat ccggtaacag cggcacagac aggtaccggc aagtatatct
     3721 atggggcgct gacgggcatc ctggtggtga ttataagggt tttcaacccg gcatatcctg
     3781 agggaacgat gctggcaata ctgcttatga acgtgatggc tccgctgatt gaccattatg
     3841 tggtgcaagc aaacatcagg agaaggataa aaagagtaaa gacagcttaa ctacacagaa
     3901 tatgaaagac ttcagcaata agtatataat tatattctcg gttgtgatgg ttgtttccgt
     3961 cgccatactc ctgtctctca tggcgctgct gctccaacca agacaggaga agaacgtgga
     4021 agtggaaaaa aagatgaata ttctctcctc catcaatgtg gtgtctgaca agtcagatgc
     4081 tgtgcagctc tatgacaggc atataaagga gagcttcgtc atcaataccg aaggaaaaaa
     4141 gttcgaaggt gtggatgctt tcactgtaac ccttcgcgcc gagcagaaga aaccgctggc
     4201 agagcagtac ctgccggtct tcattgcggt ggcagacaac ggtgataccc tgcatatcat
     4261 tcccctcgag ggaaagggac tctggggtcc gatatggggt tatgtgtcac tgaagaagga
     4321 tctctccaca atcgccggcg ccactttcga ccacaagggt gaaactccgg gactgggagc
     4381 ggagataaac accaccccct ttgagagcca gtttcacgac aagagcatct atgagaacgg
     4441 caactttgtc tcggtcaggg tggtgaaagg cggcgccagg ccgggtaacc tgcacgaggt
     4501 ggatgccatc tcgggaggga caatcaccag caaagggctg gaagcaatgg tgcacgacgg
     4561 acttgaaaaa tatgagaaat acctcagcca aaacaggaag tagatatgga aaaagttgaa
     4621 aaggagccgc tgttgtcggc aaagaacagg aaactgttga caaacccgct ggggattgac
     4681 aaccccataa cggtccaggt gctggggatc tgctcggctc tggcggtgac ggtgaagatg
     4741 aaacctgcca ttgtcatggc catatcggtc acggttgtgg tggcgttgtc aaacctgatt
     4801 atagcgctga tgcgcaaatc aatacctggc cgtatcagga tcatagtcca gctggtggta
     4861 atagccaccc tggtaatcat agttgaccag ttcctgaagg cctttgcctt tgacgtgagc
     4921 aaacagcttt cggtctttgt ggggctgatc attacgaact gcataataat gggccgcctg
     4981 gaggctttcg ccatggcgaa caaaccatgg ccttcattcc ttgacggcat cggcaacgga
     5041 gccggttacg gcctgatact gatcattgtt tcgttcttcc gtgaattcct tggcagcggc
     5101 agcatttttg gctacccggt gatggagaag ctgggcgtat acaagttcgg ttacgaggat
     5161 aacgggatga tgattcttcc gcctatggcg cttataaccg tgggcctgat catctggttc
     5221 cagcgcgcac gcaacaagtc attgatcgaa aacaagtaac agcaggatca tggaaaacct
     5281 tgttaacata tttgtaaagt cgatcttcat cgacaacatg atcttcgcat acttcctggg
     5341 tatgtgttca tacctggcag tttcgaagac agttaagaca tccatggggt tgggtattgc
     5401 cgtcaccttt gtgctgctca taaccgtgcc ggtgaacttc ctgctggaac agtatgtgct
     5461 taagaagggg gcgctggtat ggcttgatga gagctttgcg actctcgatc tcagtttcct
     5521 ctcgttcatc atgttcatag cagtgattgc gtcgatagtg cagcttgtgg agatggtaat
     5581 tgagaaatcg agccctgcac tttacaattc ccttggcata ttcctcccgc tgatagctgt
     5641 aaactgtgct atccttggcg cttcgctctt catgcaggag agggagtatg agaacattgc
     5701 cgaggctact gtgttcggtc ttggctcagg gatcggctgg ctgctggcta tagtggcact
     5761 ggccgcgata agggagaaga tgcgctattc aaatgtgcct gccccgctgc gcggactggg
     5821 gatcaccttc atattaacgg gactgatggg gatagcattt atgagcttcc tgggtatcac
     5881 actataaaga aatcgtaagg aaatgatatt aggattatca attgcaggca ccgttctcat
     5941 aagcattgcg gtatttgtac tggtgatgat actgctggtg gtggtgctgt tatatgccag
     6001 gcagaagctc accccccagg gcgatgtcac tctgaagatc aatgacaggg agatggttgt
     6061 ctcaccgggg tcgagtctgt tgtcgaccat gtccaacaat ggaatcttcc tcccatctgc
     6121 ctgcggcgga ggtggcacat gcggaatgtg ccggtgccag atcaccgagg gcgggggctc
     6181 tatactaccc accgaaaccg gcttcttcac ccgtcgtgaa cagcatgaca actggcgcct
     6241 ggggtgtcag gtcaaggtgc gcgagagcat gacaatcaag gtacctgagg ctgttcttgg
     6301 agtaaaaaaa tgggaatgcg aggtggtctc caacaggaac gtcgccacat tcatcaagga
     6361 gttcgtggtg aagctgcctg aaggcgaaga cctgaacttc cgctcaggtg attacatcca
     6421 gatagatgtt ccgaagtacg atgtggagtt cggcagggat atagatgtgg acgaggagta
     6481 cagagccgac tgggacaagt tcaaaatgtg ggatctgaag cataagaaca cagaagagac
     6541 atacagggct tattcaatgg ctaacttccc ggcggaaaag aacctcatca tactgaatat
     6601 ccgtattgcc acaccaccct ttgacaatgc caggaagcag ttcatgaacg tccctccggg
     6661 gatatgctcc acctttatat tctcacgcaa gccgggtgat aaggtttcaa tcagcgggcc
     6721 ttacggcgag ttccacatca agcatactga taacgagatg gtgtatgttg gcggcggtgc
     6781 cggcatggct cccctgagat cgcatatatt ccacatgctc aagacagaga agtcgggacg
     6841 caagatatca tactggtacg gtgcccgctc gaagagagaa atcttctatg aggatgagtt
     6901 ccgtgagctg gaaaagaagt tccccaactt tacattcaat attgccctct ccgagccgtt
     6961 gccggaggat aactggacag gatacaaggg attcatacat aaggtactgc ttgacaatta
     7021 cctgagcagg cacaagcttc ccgaagagat tgagtattat ctctgcggtc ctcccgtgat
     7081 gaatgccgcc gtgctgaaca tgcttgacaa tctgggtgta ccggaggaga atattgcgtt
     7141 tgatgatttt ggaggctagt tggatttgcg atttgcgatt tacgatttgc gatttgcaga
     7201 attgcgaact gcgaattgga aatgtcaatc cctggctcga gtttaagaca ttatgcgtat
     7261 aactctgttc tgttccgcca ttgcactgat gatggcatcc tgctcaccgc agcctgagta
     7321 tgccgagtta aacggtttca cccagggaac cacttatcac atcgttgtgg agaaaattcc
     7381 cggtctcgat gtaatggcgt tgcggcagga tattgagaat ctcttcaccg gaattgacaa
     7441 ttcactgtca atctataatg attcctcggt tatctctgcg ataaatttta accgtagtga
     7501 cctgacagac actcttttcc gggaggtatt ccgggcttct gaagtgattt ctgctgagag
     7561 cggcggtctg tttgatatta ccattggtcc gttggtgaag tcatggggct tcggccctga
     7621 cgccatgaag cggtttaatc cgtccatgct tgattcgctt atgtcactgg ttgggatgaa
     7681 taaggttcgt ctggaaggtg accgcatcat caagacagac cccggcatgt ttattgacgt
     7741 gaatgccata gcacagggtt ataccgttga ccttgcagct gacctaatcg ttcgcatggg
     7801 aataaaacag tgcctggtgg aggtgggagg tgaagtccgg acagcaggag acaagaacgg
     7861 gatgggctgg aaggtgggga tcgatgcccc ggccgacggg aatttcaccc cgggtgcaga
     7921 catccaggcc acgatcaggc ttgatgacag ggcgctggca acgtcaggca actacaggaa
     7981 attttacgtg gagaacgggg taaaatactc ccataccata gatccgagaa cgggttaccc
     8041 ggtaagacac accctgctca gcgccactat cattgccccc acgggtgccg tggcagatgc
     8101 ctgggcaaca gcctgcatgg tgggagggaa ggatgcggcc attgccttca ttgagaagta
     8161 tgattttctt gagggatacc ttatttactc tgacgaaact ggcgcaatga agagctgggt
     8221 ttctgaaggg atcaggaaaa tgatccttac ggaataagag gggccgggaa tatacaatgc
     8281 tttaattctg agtgccctac gggcaaaaca actgtacgaa atgtccataa aagtgacaga
     8341 ctatattgcc tgcagcaatt accactagtc actgttgact gcgcagccac agcacccttc
     8401 gctgtcgatg ggattgcagc cggtgtcggt gctctttgca caggttatgc cacgcttacg
     8461 catctccttg ttgtgaccca catgggtgtc aggaaactgc cccttcggct tgagcagtat
     8521 tgatatggcc aggcctgcca tcgagaggcc gaccaatatg gctgatatta tcaaaagctt
     8581 taacagcata atttttctct ccgcaatgca aatttacaac taaatcacaa accctccgac
     8641 cctccaaccc tccgaccctc cgaccctccg accttccgac cctcagacct tccgaccctc
     8701 cgaccctccg accctccgac cctcagaccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnt ccgaccctca gaccttccga ccctccgacc ctccgaccct ccgaccctca
     8881 gaccttccga ccctccgacc ctccgaccct ccgaccctca gaccctcaga ccttccgacc
     8941 ttccgaccct cagaccttcc gaccctcaga ccctccgacc ctcagaccct cagaccctcc
     9001 gaccttccga ccctcagacc ttccgaccct cagaccctca gaccctcaga ccttccgacc
     9061 ctccgaccct ccgaccctca gaccttcaat taaaacaccc ctgccatcac tttgttcggt
     9121 tatggtgtgc ctgtcagacc tcctcctccc tgtatagctc cctgtcggcg gcatcagcct
     9181 cctcaagcac tttcgcatgc tcttcagggg tatacttcct gatcttatat ccaatggcag
     9241 caatgaaaac gctcatgaac gggctgatga tattgaagaa gcagtagggt gcgtacgccc
     9301 aggtggaaac gcccagcacg cctgactggg tggcgccgca ggtgttccat ggtatcagca
     9361 ctgacgttac cgtcccggag tcctccagtg aacgactcag aagttcaggc ttgaaaccca
     9421 tgtcgcggaa tttcttcgca tacatcctgc ccgggattac tattgagagg tactggtcag
     9481 atgccgtcac attgaagaag aggcatgtcg ccacggttga accaacgagt gtgccggcat
     9541 tgtgaacaaa cttcatcagt tttccggtga tggtcttcag cataccgcag gcctccatga
     9601 ttcctccgaa gaccattgca cagatgatga gccagacggt attaagcatg ccggccatac
     9661 cacgtgtctt catcagttca tcaaccgacg gatcaccggt tgttacagct actgacccga
     9721 acatagtctt cataatggct atatacgcct gcttcacgaa cgatccgccc gtggctgcta
     9781 cctcctcgag gatgtgaggc tgaaagatga ttgcaaatac agcagcaaga agggttcctg
     9841 caagcagtga gggaagtggc ggtacctttt taacgataat atagataagc atggccggca
     9901 ccagcagcag gacaggcgtc acgttgaact tcgcctttat tgcggcctgc acggcatcgg
     9961 cattggtggc gcctccctgg agatcaatgg tgaatccgat tatcaggaaa ataaccatag
    10021 ttataatcag tgacggcgtt gtggttatca ccatataccg tacgtgcgtg aacagatctg
    10081 tgcctgcaac tgccggtgca aggttggtgg tatcagacag gggtgacatc ttgtcaccaa
    10141 aatatgcacc tgagatgatt gcaccggcaa tcattgcctc actgaaaccc agcgctttgc
    10201 ctatgccaag cagggcgact cctattgtcg caacagttga ccatgaactt ccggtggaga
    10261 cagatacaac tgcgcagatg atcaccgacg caaaaaggaa gatcttgggg tgaaggatct
    10321 caagaccgta atagattagc atcggcacca ctccgctgac catccatgtc ccggccaggg
    10381 agccgatcag aaagaggatc aggattgctg gcatggcagt gcctattccc ttgatgacgc
    10441 gttctcggat aaacagccac ctgtatccga gatttactgc aataataccg gcaactgcag
    10501 cagcaagaat aagcgccatc tggttcgcac ccgagagggt gtcatcacga aagaatttta
    10561 cattgagggt aaggaaaaca atcagcacgg cgattggcag aagcgactgc agcatcgtcg
    10621 gtgttttgta ctgtttcaga gacatgaatg ctttttagcg ttggatcgtg caatttacta
    10681 ttttccgcga tttttcttga atatgccgct aaaagatgtt attttgtatg gtcaattttg
    10741 ctgacttatg cctcaatatc ctgacatttt cagcataaat cccgaagaca aaatcccggg
    10801 aaagggcagg gtactgattt cggaaccctt cctgaccgac cgtttcttta accggaccat
    10861 cgtgttcctt accgagcaca cccctgaggg caccgtcggg tttatcctga acagggtgac
    10921 agacctgatg gtgagcagcg ccgtggaggg atttgacgga tgggacgagc acctttcggt
    10981 gggcggtccc gtttctcctg acacgctgca ttaccttcac tccctcggca cgaaggtgcc
    11041 tggaagcgtg ccggtgatac cgggaatctg gtggggtgga gagatcgatg ccctgcgcaa
    11101 cctgattgcc accggaaccg ttgggcgcga ccaggtgcgc ttcttcctgg gttactccgg
    11161 ctgggagaag gggcagttga agcgtgagct gaaggagaac tcatgggtga ttgcaaaggt
    11221 ccctccggag gttatcatga aaagccgcgg caacgtatgg cgtgacgtgc tgaggtcatt
    11281 aaacaacaaa taccggatct gggcggaatt cccggaatca cctgagatga actgaccctg
    11341 ccggcatcac tgccagggac cgacagccgc agaattacgg tgacaccggc aaaccgcagg
    11401 acaggcacgc aaatcagtgc attatcttga aaaccagctc gcgcagcagg tcgccggcgt
    11461 cgctgccggc ctcgccgaac cccttggcct tcagatcata tgaacgtacc agcgagatga
    11521 tgttcataca ggcccccaca ctgtacagag ctgctgcagc cttgaaatcc ctcacatatc
    11581 cgggataagt cttcagcatc gcaacaacgt tgccgtcact cttgtccttc aggctgtggt
    11641 agataaggac gcgactgaag ctcgagaaga gcaccgggaa gatcagcgga atgatctcct
    11701 tcttcgaatg gcctgcatag tagttcacca ccctgg
//
LOCUS       DAOPXU010000084        11667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_6273_length_11667_cov_10.136841, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000084 DAOPXU010000000
VERSION     DAOPXU010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6273_length_11667_cov_10.136841"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1480
                     /locus_tag="PKK03_08900"
     CDS             <1..>1480
                     /locus_tag="PKK03_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNX84558.1"
                     /translation="ATPLHDIVAGLCFALARNFRSTVARRKDMKKPVLFTGGVAANAG
                     MVRAFREVLDLSQEELIIPEHHASMGAIGAVLYSRLEGHQGNGFTGLEKLEKYLHRST
                     ATFVSLPQLKESAALYNKEVKIKSNGQGKLRVYLGIDVGSLSTNVVLIDPEHNVVARR
                     YLPTAGKPLEAIRKGLTEIYDEAGNFVEVIGAGTTGSGRYLTGDFIGADAIRNEITAQ
                     ATAAIDYDPTVDTIFEIGGQDSKYISIENGVVVDFEMNKVCAAGTGSFLEEQAEKLNI
                     KIVGEFGKMALSSKSPVKLGDRCTVFMESDLNTFMQKGARDENLVGGLAYSIVYNYLQ
                     KVVADRRIGNRIFFQGGVTNNKAVVAAFEQVLGKKIIIPPHFDVTGAIGAAILAQKSM
                     TEGQKTTFKGFGIRNASYEISRFVCNGCVNHCEISSVKIAGEKKPLFYGGRCEKWETD
                     SRKKKNDNIPNLFLKRTEMLMGDYREEEPDGRPSVGIPRALMV"
     assembly_gap    1481..1580
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1581..3515
                     /locus_tag="PKK03_08905"
     CDS             <1581..3515
                     /locus_tag="PKK03_08905"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HNX84559.1"
                     /translation="TSNSNCPWIQSFPFMVRAALRDRVDEKKFLTPTLHFRYFERALT
                     KEMISFFGDKMGFDSSRVKKALLQADGMQTAFERGVAEYGRKALIEIPEGCRPAVILG
                     RPYNSTDPHLNLSLAEKLIAQNVMPIPLDMLDLSVADLYKNYRNMYWPNGQKIIAAAK
                     HVARTDGLYAVYISNFRCGPDSFIWHYVTEELKGKPFLHLEVDEHSADAGMVTRIEAF
                     LDSLEGSEKNARKAVEVIRPRPSPSEPPSNRTLYFPYMHDGARFVAAAARSVGIPSEV
                     LPMQTEEDIALGRKYTSSKECFPMICTTGSFLKKLMEPGADPSKMSFFMPDHNGPCRF
                     GQYNQFQRILFDRLGFRDAELVTPSNDTSYADLAGDRSQRFRINAWKGFVAFDFIRKL
                     YRETRPYELNPGESEAVYLDAMKKAEASIEKNCRGLRRILVGASSAFMAVKVDKSKKK
                     PVIAVLGEIFMRDNAGCNGNIANRLEALGAEVLIGPFSEWITYSTHRYTRDSRWKNDT
                     KGVIRSKIQGIGQEVLVDYLLRGIRKVSDTSKDVSLHEILRLSNRYVSEFYDGDPPIA
                     LGMSSALAGRGVSGIAAILPFTCMPGTLIASVSDSFRKDHNNIPFLNIPYDGQDSVSL
                     ETRLQAFFFQVREYAEAHQQ"
     gene            complement(3484..5652)
                     /locus_tag="PKK03_08910"
     CDS             complement(3484..5652)
                     /locus_tag="PKK03_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNX84560.1"
                     /translation="MKTKLFTIAMLLAMATPAALADEGMWIPVLIEKYNIKLMQENGF
                     KLTAEDIYSINRASMKDAVVLFGGGCTGEFISDKGLIITNHHCGYGYIQNHSTLEHDY
                     LRNGFWAASNAEELANPGLSVTILKYMEDVTDKVLKGVTDDMDKEKRDEIINANITAI
                     NAEAVKGTHYRSAVRPFFLGNQYFLIVNESFRDVRLVGAPPSAIGKFGGDTDNWVWPR
                     HTGDFSLFRVYADKENKPAAYSAENVPYHPAYHFPVSIAGVKEGDFTMVFGYPGRTQE
                     YAPSYHISMLKDVIYPKMVDMRGKKISIMEQAMAKDPLVRLKYSGKSFGLANGWKKSI
                     GEIQSLNRMNGVARKQAFEKEFMKWVAADPERVRKYGKILDEYADIYSTYTHNYLVNS
                     YTNEVFGSAGVEPASLAGAFRRAVEMASQKDPGLDKELKRLQEYADGYFRNYDRNVSE
                     QLFVAVMDLYGKNIDREWQTDVYKALAESCRGDFGMVAGKLFDKSIFTDENRVKALIS
                     KFDPKKVQKDIFYRMAVSASDLTASRLRGELSAADARLAELNKLYMAAQMEQGGDKVF
                     YPDANSTLRLAYGKVMGYDSRDAVYHEHQTTLTGVMEKDNPEIYDYDVPDRLKELYEK
                     KDFGRYGYDGDMPVCFIANNHTTGGNSGSPVLNAEGHLIGVNFDRAWEGVASDMMYNP
                     QQSRNISIDIRYALFLIEKLGNAGYLIDEMTIVGVPQHTP"
     gene            complement(5649..6266)
                     /locus_tag="PKK03_08915"
     CDS             complement(5649..6266)
                     /locus_tag="PKK03_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HNX84561.1"
                     /translation="MFSDRLQNFDLILASASPRRRQLMEDAGFRFRVAVTGFEENWPA
                     HLRGKDIAEFLAAGKASSWDEPLAPGQVLITADTIVWCHGENLGKPAGDAEAISFLRQ
                     LSGCRHEVITGICLRDSKKEMVFSVTTGVTFREMSDDEIEYYVKNYRPLDKAGAYGIQ
                     EWIGLRGITTIEGSYYNVVGMPVSELFTALLQFTGNEIRNKTRNI"
     gene            complement(6268..7278)
                     /locus_tag="PKK03_08920"
     CDS             complement(6268..7278)
                     /locus_tag="PKK03_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HNX84562.1"
                     /translation="MKHSRGERLKDLLDLVRLPNLLVVALTMMLMRYAIIRPLLGAIL
                     VTMAGDPATLVAMDFRLGWFDFLILVISTCLITAAGYVINDYFDIRADLINRGTIIVG
                     NTITRRMAMMYHNVFNLLGVIGGIYVSARIGYVWLGIFFILISGLLYFYSATYKRQFL
                     IGNIIVALLVAMVPMLVVVYDAAPVYVYYSQASSGFPGVAILFWWVGGFALFAFLTTL
                     IREIIKDMEDCDGDRETGRKTLPVISGLTVCRVIVVLLSVVTVALLYYVWSRHLRDTI
                     TLVYISLLLAVPLVAVIVRVLTSGEKKHLTLASRLMKLIMLAGILYSLVAGAIISSGK
                     IL"
     gene            complement(7259..7780)
                     /locus_tag="PKK03_08925"
     CDS             complement(7259..7780)
                     /locus_tag="PKK03_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNX84563.1"
                     /translation="MTNFKEELRGVKAFVFDIDGVLSTQTIALSVWGIPLRTVNLRDG
                     YAIQLAVKKGYHVGVISGANSKEYIKRLKTLGVNDIFLNSRTKKESMKELAERWNVDL
                     KSVLYMGDDIPDFEVMKMVGLPACPADADSEIKQIAVYISDKNGGEGCVRDVIEQALR
                     LHKNWMDYEAFSW"
     gene            complement(7777..8559)
                     /locus_tag="PKK03_08930"
     CDS             complement(7777..8559)
                     /locus_tag="PKK03_08930"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNX84564.1"
                     /translation="MSTYNISFIGAGNVAESLATGLASAGHRIVSVASRNGISARELA
                     KKTGAGYRLDLTVPGECDLLLICVTDKTVAEVAASIVISPHTVIAHTAGSIPIEALGR
                     ASGAGVFYPLQTFTKAFPPDLRKVPFFIEATDSLAMEVLRNTGESIGAGVWECDSESR
                     KKLHLAAVFTNNFSNFMMTAGESVAREAGIDPVLLRPLMEETVRKALRTGPEAAQTGP
                     AVRHDTGTVKSHIELLSFSPQYQKLYRLVSRMIAGHYRKREK"
     gene            complement(8594..8995)
                     /locus_tag="PKK03_08935"
     CDS             complement(8594..8995)
                     /locus_tag="PKK03_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84565.1"
                     /translation="MKRNRILATLALVFAVAALVAVTAGCKKQPKCGCDGDALDSLNL
                     SNVYITYDAVNKTARFSTIWDQYSIYYFCNPSQWISELTKFAQGEEILVSGEYFYECN
                     YLMNSSNSYYYSYMRIYQVKVTAVAAHEWGK"
     gene            complement(9101..9418)
                     /locus_tag="PKK03_08940"
     CDS             complement(9101..9418)
                     /locus_tag="PKK03_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNX84566.1"
                     /translation="MSDIKNQGQFNIELSEDVAEGVYSNLAIITHSPAEFVIDFVRIM
                     PGVPKSKVKSRIILTPEHAKRLVAALSDNIAKYEAVHGPIREVKGSGPVMPVTFGGPT
                     AQA"
     gene            complement(9431..>11667)
                     /locus_tag="PKK03_08945"
     CDS             complement(9431..>11667)
                     /locus_tag="PKK03_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNX84567.1"
                     /translation="ERYNQIIDIWTHVNARLTQSLMKQLSNDKQGFNSVYMMLDSGAR
                     GSKEQIRQLSGMRGLMAKPQKSGATGSEIIENPILSNFKEGLSVLEYFISTHGARKGL
                     ADTALKTADAGYLTRRLVDVSQDVIITEEDCGTLRGLVATAIKNNEDIVESLYERILG
                     RTTVHDIYNPLTGDLIVSAGEEITEEVAGNIDQSPIEQVEIRSVLTCESKKGVCAKCY
                     GRNLATGRMVQKGEAVGVIAAQSIGEPGTQLTLRTFHVGGTASNITTESSLIARYGGI
                     AVIEELRTVAKKDPEKGNINIVIGRLAELRIVDKTTGIALTTHTIPYGSRLYVKPNQE
                     IQKGELICEWDPFNAVIISEVSGKVAFDYLIEGVTFREESDEQTGFKEKVIIESRDKT
                     KNPTIKIMSPEGVEIKSYNLPVGAHLVTDLNKLVEAGDTIVKIPRAVGKSGDITGGLP
                     RVTELFEARNPSNPAIVTEIDGEVTYGKIKRGNREITITSKSGEMKKYLVPLSKQILV
                     QENDYVRAGTSLSDGAITPSDILAIKGPTKVQEYIVNEVQEVYRLQGVKINDKHFETI
                     VRQMMRKVEIIDPGDTKFLERQIVDKLEFMDENDWVYGKKIVLEPGDSQTLKPGMIIT
                     ARKLRDENSLLKRRDLKLIDARDAVPATSQQVLQGITRAALQTNSFFSAASFQETTKV
                     LNEAAILGKVDFLEGLKENVIVGHLIPAGTGLREYNNIIVGSLDDYESLVHAGQSPSE
                     VEQG"
BASE COUNT         2706 a         3057 c         2930 g         2874 t
ORIGIN      
        1 ttgccacacc gctccatgat atagttgcag ggctgtgttt cgcccttgcg cgaaacttcc
       61 gcagcaccgt agccaggagg aaagatatga agaagccggt gcttttcacc ggcggtgttg
      121 cagccaatgc cggaatggtc agggcgttca gggaagtttt ggatctcagc caggaggagc
      181 tcatcatacc tgaacaccat gcatcaatgg gtgcgatagg tgccgtgctc tactcacgac
      241 ttgaaggtca ccagggcaac ggcttcacag gacttgaaaa acttgagaaa taccttcacc
      301 ggagcacagc cacgtttgtc agcctgcccc agctgaaaga atcggctgca ctatacaaca
      361 aagaggttaa gataaaaagc aacggccagg gtaagctgcg cgtttacctg ggtattgacg
      421 tcggttcact cagcaccaac gtggttctga ttgaccctga acacaacgtg gtggcccgcc
      481 gttacctgcc tacggcagga aagccgctcg aggccatcag gaaaggactt accgagatct
      541 acgatgaggc tggcaatttt gttgaggtca tcggggcagg aacaaccggc tcgggaaggt
      601 accttacagg tgacttcata ggggctgacg ccatcaggaa cgagatcacc gcacaggcaa
      661 ccgcagcaat agactatgat cccactgtgg acaccatctt cgagattggc gggcaggact
      721 ccaagtacat cagcattgaa aacggagtgg tggttgattt cgagatgaac aaggtctgtg
      781 ctgccggcac aggctcattc ctcgaagagc aggccgagaa gctcaacata aagatagtcg
      841 gcgagttcgg gaaaatggcc ctcagcagta aaagtcccgt taagcttggt gaccgctgca
      901 ccgtcttcat ggaatctgac ctgaacacat tcatgcagaa gggagccagg gatgagaacc
      961 tggttggcgg actggcttat tccatagtct ataactacct tcagaaggtg gtggcagacc
     1021 gcaggatagg caaccgcatc ttctttcagg gtggcgtaac caacaacaag gcggtggtgg
     1081 ctgctttcga gcaggtgctt gggaaaaaga taattatccc gccccatttc gatgtcacag
     1141 gagccatcgg tgctgcaatt ctggcacaga agtcaatgac cgaaggacag aagactacat
     1201 ttaaggggtt cggcataagg aatgccagct atgagataag ccggtttgtc tgcaacggct
     1261 gtgtcaatca ctgtgagatc agcagcgtga agattgccgg ggagaaaaaa ccacttttct
     1321 acggcggcag gtgcgaaaaa tgggagacag acagcaggaa gaaaaaaaat gataacattc
     1381 ccaacctctt tctgaagagg acggagatgc tgatgggcga ttaccgcgag gaggagcctg
     1441 acggcaggcc ttcagtcggc atccccaggg cgctgatggt nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn acatccaact ccaactgtcc gtggattcag tcgtttccgt
     1621 ttatggtcag ggcagcgctc cgtgaccggg ttgatgagaa gaaattcctc acccccaccc
     1681 tgcatttcag gtattttgag cgggctctga caaaggagat gatttcgttt ttcggtgata
     1741 agatgggatt tgattcctcc agggtgaaaa aggcgcttct gcaggctgac ggcatgcaga
     1801 cagcctttga gagaggcgtg gcggaatacg ggcgtaaggc cctcattgaa attcccgaag
     1861 gatgcaggcc tgcagttatt cttggcaggc catacaacag cactgatccg caccttaatc
     1921 tgagccttgc agaaaagctc atcgcacaga atgtgatgcc tattccgctt gacatgcttg
     1981 acctttccgt tgctgacctt tataagaact accgtaacat gtactggccc aacggccaga
     2041 agatcattgc ggcagcaaag cacgttgccc gtacagatgg tctgtatgca gtatatatca
     2101 gtaatttcag gtgcggccct gattcattca tatggcatta cgtcaccgag gagctcaagg
     2161 gaaagccctt ccttcacctg gaagtcgatg agcactctgc tgacgcaggc atggttaccc
     2221 gcatagaggc atttcttgac agcctggagg ggtcagagaa gaatgcaagg aaggctgttg
     2281 aggtcatcag gccccggccc agcccttctg agccaccctc gaacaggacc ctctatttcc
     2341 cgtacatgca tgacggcgca cggtttgttg ccgccgcggc gaggagtgtc gggatcccct
     2401 cggaggttct gcccatgcag acagaagagg atattgcact gggaaggaag tatacctcat
     2461 cgaaggagtg tttcccgatg atctgcacca ccggcagctt cctgaaaaag cttatggagc
     2521 cgggagcgga cccgtcgaag atgagcttct tcatgcctga ccataacgga ccatgccgtt
     2581 tcgggcagta caaccagttc cagaggatac tatttgacag gcttggcttc agggatgcag
     2641 agctggtcac accttcaaat gacacctcct atgccgatct ggcaggagac cgcagccagc
     2701 ggttcaggat caacgcgtgg aaagggttcg tggcgtttga cttcatcagg aaactgtaca
     2761 gggagacccg gccatacgag ctcaatccgg gcgaaagcga ggctgtctac ctggatgcca
     2821 tgaaaaaggc cgaagcctcc atcgagaaaa actgcagggg ccttcgccgg atactggtcg
     2881 gggcttcatc ggccttcatg gccgtaaagg tcgacaagag caaaaagaaa ccggtgatag
     2941 ccgtgcttgg tgagatcttc atgcgtgaca acgcaggatg caacggcaac attgccaacc
     3001 ggcttgaagc actgggcgcc gaggtgctca ttggaccctt ctccgaatgg atcacctatt
     3061 caacccaccg ctatacacgt gacagccgct ggaagaacga cacgaagggt gtcatcaggt
     3121 cgaagataca gggcattggc caggaggttc ttgttgatta cctgctgaga ggcatcagga
     3181 aggtgagtga tacctccaaa gatgtgtcgc ttcatgaaat actgaggctg agcaacaggt
     3241 acgtaagcga gttctatgac ggtgatccgc caatagccct cgggatgtct tctgcgctgg
     3301 cagggagagg ggtgtcagga atagcagcaa tcctgccctt cacgtgtatg ccgggaacgc
     3361 ttatcgcttc tgtcagtgat tccttcagga aggatcacaa caacattccg ttcctcaaca
     3421 taccctatga cgggcaggac agcgtatcac ttgagacaag gctacaggcc ttcttcttcc
     3481 aggtcaggga gtatgctgag gcacaccaac aatagtcatc tcgtcaatga ggtaacctgc
     3541 gttgccaagc ttttcaatca ggaaaagcgc atacctgata tctatgctga tattacggct
     3601 ctgctgaggg ttgtacatca tgtctgatgc caccccttcc catgcacggt caaagttgac
     3661 ccctatcagg tgaccctcgg cattaagcac cgggcttccc gagttgcctc ctgtagtgtg
     3721 gttattggca ataaagcata ccggcatatc gccgtcatat ccgtacctgc cgaaatcttt
     3781 cttctcatac agttccttca gcctgtcagg cacatcgtaa tcatagatct ccgggttgtc
     3841 cttctccatc acaccggtga gggtcgtctg atgctcatgg taaacggcat cacgtgagtc
     3901 ataacccatc acctttccgt aagccagcct tagggttgag tttgcatcag gataaaagac
     3961 cttgtctccc ccctgctcca tctgggcggc catatagagt ttgttgagct ctgccagcct
     4021 ggcatcggca gctgacagct ctcccctgag gcgtgatgca gtcaggtcag atgccgagac
     4081 agccatccgg tagaagatat ccttctgaac ctttttggga tcgaatttgc tgatgagtgc
     4141 cttaaccctg ttctcgtcgg taaagattga cttatcgaaa agcttaccgg ccaccatgcc
     4201 gaaatctccc ctgcatgatt cagccagcgc cttgtagaca tcagtctgcc actccctgtc
     4261 gatgttcttc ccgtaaaggt ccattacagc cacaaacagc tgctccgaaa cgttgcggtc
     4321 atagttgcgg aagtatccgt cggcatattc ctgcagtctt ttcaattcct tgtcaagccc
     4381 gggatccttt tgggaggcca tttccactgc tcttctgaag gctccggcaa gtgatgccgg
     4441 ttctacgcct gctgagccaa acacctcatt ggtgtaggag tttacaagat aattgtgtgt
     4501 gtaggtgctg tagatgtcag catactcatc gagtatctta ccgtactttc ggacacgttc
     4561 cgggtcagct gccacccact tcatgaactc cttctcaaaa gcctgcttgc gggctacacc
     4621 attcatcctg ttaaggctct ggatttcacc gattgatttc ttccagccgt tggcgaggcc
     4681 aaatgattta cccgaatact tcagcctgac cagcgggtcc tttgccatgg cctgctccat
     4741 gattgatatc ttcttgccgc gcatgtccac cattttcggg tagatgacgt ccttcagcat
     4801 gctgatgtga tatgacggag catattcctg tgtccttccg gggtatccga agaccattgt
     4861 aaaatcgccc tccttaactc cggcaattga caccgggaag tggtatgcgg gatggtaggg
     4921 cacattctca gctgaatatg cagcaggctt gttctccttg tcagcataaa ccctgaacag
     4981 ggaaaagtcg cccgtatgcc tcggccatac ccagttgtca gtgtcgcctc cgaacttgcc
     5041 gatggctgac gggggcgctc ccacgagtct gacatcacgg aaagattcat tgacaatcag
     5101 aaagtactgg ttaccaagga agaaaggcct gacggccgaa cgataatggg ttcctttgac
     5161 tgcctctgcg ttgatggcag tgatgtttgc gttgatgatc tcatcacgtt tctccttgtc
     5221 catgtcatca gtcacccctt tgagaacctt gtcggtgaca tcttccatat acttgaggat
     5281 ggtgacggag agtccgggat ttgcaagctc ttcagcattg gaagcagccc agaatccgtt
     5341 tctcaggtag tcatgctcca gagtggagtg gttctgaatg tacccatagc cacagtggtg
     5401 gttggtgatt atcagccctt tgtcagagat gaactcgcct gtgcaacctc ccccgaatag
     5461 caccacggcg tctttcattg atgccctgtt gatgctgtag atatcttcag ctgtaagctt
     5521 gaagccattc tcctgcatca gtttgatgtt gtacttctca ataagcacag ggatccacat
     5581 cccctcatct gccaatgctg ccggtgtggc catggcaagg agcatcgcaa tagtgaaaag
     5641 tttcgttttc atatgtttct ggttttgttt ctgatttcgt ttccggtaaa ttgaagaagg
     5701 gctgtaaaga gttcgctaac cggcattccc acaacattgt aataagaccc ttctattgtt
     5761 gttatgcccc tcaggcctat ccattcctgg atgccatatg cgccggcttt gtcgaggggg
     5821 cggtagttct taacgtagta ttcaatctcg tcatcactca tttccctgaa ggtgactccg
     5881 gtggtgactg aaaagaccat ttctttttta ctgtctctca ggcatatacc tgtgatcacc
     5941 tcatgcctgc acccggaaag ctgcctgagg aaactgattg cttccgcatc accggcaggc
     6001 ttgcccaggt tctcaccatg gcaccagaca atggtgtcag cggtgatcag cacctgcccc
     6061 ggagcaagcg gctcatccca tgaggacgcc ttgcctgccg ccaggaactc tgcgatatct
     6121 tttcccctga gatgagcagg ccagttttct tcaaagccgg tgacagccac cctgaacctg
     6181 aatccggcat cttccattag ttgcctgcgc cttggtgatg ccgaggcaag gataaggtca
     6241 aagttctgca acctgtctga aaacattcta aagaattttc cctgaagaga ttattgctcc
     6301 tgcaacaagt gagtagagta ttcctgccag catgatgagc ttcatcagcc tgcttgcaag
     6361 ggtaagatgc ttcttttccc ccgaagtgag caccctgaca atcaccgcca ccagcggaac
     6421 cgccaggaga agcgagatat acacaagggt gatggtgtca cggagatgcc tggaccatac
     6481 ataataaagc aacgccacgg ttacgactga aagaaggaca acaatgactc tacagactgt
     6541 gagccctgat atcaccggca gcgtttttct gcctgtctca cggtcgccat cacaatcttc
     6601 catatccttt attatctcgc ggatgagggt tgtcaggaat gcaaacaggg cgaatccgcc
     6661 tacccaccag aaaagtatgg ctacacccgg aaaaccggag gaggcctgtg aatagtatac
     6721 ataaacaggt gctgcatcat acaccactac cagcattgga accattgcaa ccagcagcgc
     6781 cacgataatg tttccaatca ggaactgccg cttataggtg gccgagtaga agtaaagcag
     6841 cccggatatc agtataaaga agattccaag ccatacataa cctatacgtg cagaaacgta
     6901 tatcccgccg ataacgccca gtaaattgaa cacgttatga tacatcattg ccatgcgcct
     6961 tgtgatagtg tttccgacaa tgatagttcc cctgttgatc aggtctgccc ggatatcgaa
     7021 gtagtcgttg atcacatagc ctgctgcagt gataaggcat gttgaaatta caagtatgag
     7081 gaagtcaaac cagccgaggc ggaagtccat ggcaaccaaa gttgccggat ctccggccat
     7141 tgtcaccagg atggcgccca gcagcggcct gatgattgcg tatctcatca gcatcattgt
     7201 cagcgccact accagaaggt ttggcagcct tacaagatcc agaaggtctt ttaatctctc
     7261 accacgagaa tgcttcatag tccatccagt ttttgtggag tctcagcgcc tgctcaatca
     7321 catcgcgcac gcatccctct cccccatttt tatctgaaat gtaaacggca atctgcttta
     7381 tttcactgtc ggcatcggcc ggacaggccg gaaggccaac catcttcatc acctcaaagt
     7441 caggaatgtc gtcacccatg tacaggacac tcttcaggtc tacattccac cgttctgcca
     7501 gttccttcat gctctctttc ttggttcgcg aattcagaaa aatgtcatta acaccaagtg
     7561 ttttaagtct tttgatatat tcctttgaat tagcgccgga aataaccccg acgtgatatc
     7621 cctttttgac agccagctgg atggcatatc cgtccctgag gttgactgtc cggagtggta
     7681 tgccccatac cgagagagcg atagtctgtg ttgacagtac tccgtcgatg tcaaagacaa
     7741 aggcttttac tccccggagt tcttccttga aattcgtcat ttttcccttt tcctgtaatg
     7801 tccggcaatc atcctgctga caagccggta gagtttctgg tattgtggtg agaaagatag
     7861 caattcgatg tgacttttca ctgtgccggt gtcatgtcgc acagcaggcc cggtctgggc
     7921 ggcttcaggg ccggtgcgga gagccttgcg caccgtctcc tccatcaggg gtctgagcag
     7981 taccgggtca atgccggctt ccctggcgac tgactcaccg gctgtcatca taaagtttga
     8041 gaagttgttt gtgaagacgg cagccaggtg aagctttttt ctgctctctg agtcacactc
     8101 ccacaccccg gccccgatgc tctcacctgt attcctcagc acctccatgg ccagactgtc
     8161 agtggcctca atgaagaacg gcaccttgcg cagatcaggc ggaaaagctt tggtaaaggt
     8221 ctgcagcgga taaaatacac ccgcaccgga tgcccgcccg agggcttcaa tcgggatact
     8281 gcctgctgtg tgggcgatga ctgtatgagg ggatatgacg attgaggcag caacttcggc
     8341 aactgtctta tcggtgacac agatcagaag gagatcacat tcgcccggca cagtaagatc
     8401 cagccggtat ccggccccgg tttttttcgc cagctcacgt gcgcttatgc cgtttcttga
     8461 ggcaacggat actatcctgt gacctgctga tgccagccct gtggcaagtg actccgccac
     8521 attgcctgct ccgatgaagg atatgttgta ggtagacacc gttgctggca gatcattgcg
     8581 tcaaattgca gcgttacttt ccccattcat gggcagccac cgcagttacc tttacctggt
     8641 agatcctcat atagctgtaa taatatgagt tactgctgtt catcaggtaa ttgcactcat
     8701 agaagtactc ccctgacacc agtatctcct ctccctgggc gaatttggtc agttcactta
     8761 tccattgcga gggattacag aaataatata ttgaatactg atcccagatt gtggagaacc
     8821 gcgcagtctt gttgactgcg tcatatgtga tataaacgtt tgacaggttc agggagtcaa
     8881 gggcgtcacc gtcacaaccg cattttggtt gtttcttgca gccagccgtc actgcaacca
     8941 gtgcagcaac tgcaaaaacc agggccagtg tggccaatat cctgtttctt ttcatgattc
     9001 tgagctgttt agacactgca aaataatgct aattttcctt ccctccaaac aaacaagccg
     9061 gttgttttca caaccggctt atcttcagta tgttcatgaa tcatgcctgt gctgtcggtc
     9121 cgccgaaagt gaccggcatg acagggcctg atcccttgac ttcgcgtatc gggccgtgaa
     9181 cagcctcgta cttggcaata ttgtcactca gcgctgccac aagccgcttg gcatgctcgg
     9241 gggtcaggat gatcctcgac ttgacctttg acttgggtac gcccggcatg atccggacga
     9301 agtcaattac aaactccgcc ggtgagtggg ttattattgc caggttggaa tagacgccct
     9361 cagcaacatc ctcgcttagt tcaatgttaa actggccctg attcttgatg tctgacatga
     9421 tcctctggtt ttagccctgt tccacctcgc tgggactctg cccggcgtga acaagagatt
     9481 cataatcatc gagagacccg acaatgatgt tgttgtactc cctgaggccg gtgcctgccg
     9541 ggatgaggtg tccgacgatg acattctcct tgagaccctc aaggaagtcg accttcccga
     9601 ggatggcagc ctcattgagg accttggtgg tctcctggaa tgatgctgca gagaagaagc
     9661 tgttcgtctg gagtgcagcg cgggtgatac cctgaagcac ctgctgtgat gtagcaggta
     9721 ctgcatctct tgcatcaatc agcttgaggt cacggcgctt gagcagtgag ttttcatccc
     9781 tgagtttgcg ggccgtgatt atcatgcccg gcttcagcgt ctggctgtca cctggttcca
     9841 gcacaatctt cttgccatat acccagtcat tctcatccat gaactccagc ttatcaacaa
     9901 tctgtcgctc aaggaattta gtgtcacccg ggtcaattat ctcaaccttg cgcatcatct
     9961 gcctgacgat ggtctcaaag tgcttgtcat tgattttcac accctggaga cgataaacct
    10021 cctggacctc attgacaata tattcctgga cctttgtggg ccccttgatg gcaagaatgt
    10081 ccgaaggtgt gattgcgccg tcagagagtg atgtgcctgc cctgacatag tcattctcct
    10141 gcacaagaat ctgttttgac agcggtacaa ggtacttctt catctcacct gacttggatg
    10201 tgatggttat ctccctgtta cccctcttga tctttccgta ggtaacctca ccgtcaatct
    10261 cggtcacgat agccgggttg gacgggttac gtgcctcaaa gagctcggta acccttggca
    10321 gacctccggt gatgtcaccg gactttccta ctgccctcgg tatcttgacg atagtgtcac
    10381 cggcttcgac cagtttgttg aggtcggtaa ccaggtgggc acccaccggc aggttatatg
    10441 atttgatctc aactccctcg ggagacatga tcttgatggt cgggttcttg gtcttgtccc
    10501 tgctctcaat gatcaccttc tccttgaagc ctgtctgctc atctgactcc tcacggaagg
    10561 tcacgccttc tatgagataa tcaaatgcca ccttaccgga aacttcggag attatgaccg
    10621 cgttgaacgg atcccattcg caaatcagct cacccttctg tatctcctgg ttcggcttga
    10681 cataaagcct gctgccgtaa ggaatggtat gtgttgtaag cgcaataccc gtggttttgt
    10741 caacaatcct gagctcggca agacggccga tgacaatatt gatgttcccc ttctcgggat
    10801 cctttttggc aacggttctc agttcctcaa tcacagcaat accaccgtat cttgcgataa
    10861 ggctagattc ggttgtgatg ttcgaggcgg taccaccgac gtggaaggta cggagggtaa
    10921 gctgtgtacc gggttcaccg atactctgtg cggctataac accgacagcc tcacccttct
    10981 gcaccatcct tccggtggcg aggttacggc cgtaacactt ggcacagaca cccttctttg
    11041 attcgcaggt aagaaccgaa cggatctcca cctgttcaat gggcgactgg tcaatattgc
    11101 cggcaacttc ctccgttatc tcctcccctg cggagacaat aagatctcct gtaagcggat
    11161 tgtagatgtc atggacggtt gtgcggccaa ggatcctttc atacagtgat tcaactatat
    11221 cctcgttgtt cttgattgcc gtggcaacca gtccgcgcag ggttccgcag tcctcctcgg
    11281 tgatgatgac atcctgggag acgtcaacga gacggcgtgt gaggtaacct gcgtcagcag
    11341 tcttgagggc tgtgtcagca agacccttac gggcgccgtg ggtggagatg aagtactcga
    11401 gtactgacag tccttccttg aagttcgaca ggatcgggtt ctcgatgatc tccgagccgg
    11461 tagcgcctga tttctgaggc ttcgccataa gacccctcat ccctgagagc tggcggatct
    11521 gctccttcga gccccttgca cccgagtcaa gcatcatgta aaccgagttg aagccctgct
    11581 tgtcatttga cagctgcttc atgaggctct gggtaagacg ggcattgaca tgagtccaga
    11641 tatcaatgat ctggttgtac ctttcat
//
LOCUS       DAOPXU010000085        11558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_6365_length_11558_cov_9.685386, whole genome shotgun sequence.
ACCESSION   DAOPXU010000085 DAOPXU010000000
VERSION     DAOPXU010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11558
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6365_length_11558_cov_9.685386"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..1048
                     /locus_tag="PKK03_08950"
     CDS             128..1048
                     /locus_tag="PKK03_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84568.1"
                     /translation="MNSKLKILPLILGVTALAFCLAATSCKGKTRGEANKGQEEAVSP
                     DEQTMEQIKSAEKVFNALPTPLEVAQLIKEAGAKFDATLLNPVENKSNYTSSKSMALN
                     LGTYVCDLSFASMYDETSLLVNYMEAAEDMATGLGILDAIDETTIERLEQNINNRDVI
                     MEIVSETFLNSQAYLEDDEQHAVAATIIAGGFIEGLYISTSLVDMKNYKGNKLVGTIA
                     DQKMSVDDMIRLLETYTDNTAIRELLGPIHELKAIFDRIERSTTPSTAQYNEKIGMTE
                     ITGAAKSEMKQEVFLAIKDKVAEIRNSYVK"
     gene            1055..1498
                     /locus_tag="PKK03_08955"
     CDS             1055..1498
                     /locus_tag="PKK03_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84569.1"
                     /translation="MRQLIQILLVTVLLSLLVPGEAAAQCKGFAKSLCKKELGAYLHD
                     GNYHAAVLVEGEEAELYKTFYSDLEYRLAICSADGMPPVEFTVYDATGKVLYNNKDQG
                     LAKTWDFRLEASQQLKVVVKVSTFGTREQLPVSGCVAIMFGFKAK"
     gene            complement(1485..2030)
                     /locus_tag="PKK03_08960"
     CDS             complement(1485..2030)
                     /locus_tag="PKK03_08960"
                     /inference="COORDINATES: protein motif:HMM:NF033823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HNX84570.1"
                     /translation="MEDKKIILLSCGSFDRGFLKKVSGAVEDEYRCQVITEECHLDLS
                     PYYNPARRQYDGDRLLNQIPACTTSEYFKIIGLFRVDLYIPILTYIFGQAILGRQLAI
                     ASVFRLRNELYGLKQDDDILLSRTIKEVIHELGHTFGLVHCHNLSCVMRSSTYAEEID
                     LKGAALCPSCRSIYLSNPTLP"
     gene            complement(2051..3391)
                     /locus_tag="PKK03_08965"
     CDS             complement(2051..3391)
                     /locus_tag="PKK03_08965"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNX84571.1"
                     /translation="MADKISILIVDDEDSVRDSLLNWFLEDGYEVEAAENAKKALQLL
                     EERNFNIILADIKMPGMDGLELHRRIRSLNRETIVIIMTAFASVETAVQALKDGAFDY
                     VTKPFDPDDLSHLIRNAASQIALRNENEALKERVTTLEEVDDIIGHSEAIVRVLQHIE
                     SVAQSNASVIITGESGTGKELVARAIHANSSRKYFPMISVHCGALTESLLESELFGHE
                     KGAFTGATFNRKGRFEMADGGTIFLDEIATISPKMQIELLRVLETKSFVRVGGNREIT
                     SDFRVICATNRDLAAMVRNGTFREDLYYRLNVVNIDIPPLRERTDDIPLLVNHFIRKY
                     CTSMSRDLITIEPSALKHLEKFEFPGNVRELENMIERAIVIGNGREIKLKDLPLGAET
                     PENSVESLDELEKRHIEQILLKYNWNISRSARALSVDRVTLYNKIKKYDLRQKE"
     gene            complement(3402..5072)
                     /locus_tag="PKK03_08970"
     CDS             complement(3402..5072)
                     /locus_tag="PKK03_08970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX84572.1"
                     /translation="MAQRGKTDSPRESLGEKMRRYFRFSSTIYGRVVTIITLAAVVLF
                     ISFSAIFRTVYINYFNTVLKQNGNNVGAIVEGSLYKSMLENDKSTLHGTLDIINTMPG
                     IDDVNLYDQNDNLAYSSFAADARNHSNPDCISCHTDLGQMFSRNERSYMIIDAKSDCA
                     MNPNKPGERHMLIRRPILNELSCYTAACHAHSRDDEVLGSLIIRMPLRDLDSALRKSS
                     VQFLLMALLISGIIVMILIWFTRKRIKAPLLGIISASEAVSQGEVSTRLDIKPNLLDD
                     MKMVSMAFNNMLDHIDNATRELENWSKQLEYKVQKKSEELSEAHNELMNVERIASLGK
                     LSASVAHEINNPLSGILIYTKLVHKQVESQDLTAAKKQNILRQLKLIETETKRCGDIV
                     KGLLDFSRKDQEDFEERHLNEIVEGTYELMLHHVKISNIRFTVMTEAKADLVRCSANQ
                     IKQACVALLVNASEAVTENGEISIRTRNSDSTTVRIEISDNGSGIAPEDIPHIFEPFF
                     TTKQDASGIGLGLSIVHGIVQNHNGRIEVDSAVGRGTTISIILPLKTE"
     assembly_gap    5304..5403
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5404..7018)
                     /locus_tag="PKK03_08975"
     CDS             complement(<5404..7018)
                     /locus_tag="PKK03_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84573.1"
                     /translation="MKKSIIFIISLACLSAGLSGSQYAGPEQDGKNTDAVTVASSTGL
                     KALAQSWIDAYRVENPGRNVSMLPVDEYNKADIRIFEGASAGTASGESEWKMVVARDV
                     IVPVMSTDNPFYQVIMSKGIAPEKFGALLSSPTVTWGKILGTEANVPAMTNVAVDGAA
                     LGYLAGFAGIDASQVIARELPVSEIASLLRNQPATVVFCKLSDITAPGGTGFAENLMV
                     VPIDINSNGQSDYFEQFYADFNSFNRGVYIGKYPKELCNSIFCATASQMVPEAAADFI
                     RWILADGQEIAATSGLTALSGSEGMIRREMLTPVTEIVQAGNENKAGVSILVWVMAVI
                     SAISLLGFLLYRITRAGKGKISLLEEEPLKAFNPKTFVTPAGILFDKSHTWAFMEKNG
                     TVTVGIDDFLQHVTGIVTKITLKATGEKVRKGERIASLIQKGKQLDVLSPVSGTVVSR
                     NETLANNTDLLHSGPYSEGWMCGIEPDNWFNESRLMAAADKFAVQLREEFGRIRDFLA
                     TVAGANDMRLAHVVLQDGGELKEGFLEEFG"
     assembly_gap    7566..7665
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7666..9147
                     /gene="zwf"
                     /locus_tag="PKK03_08980"
     CDS             <7666..9147
                     /gene="zwf"
                     /locus_tag="PKK03_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNX84574.1"
                     /translation="GRGDLAMRKIVPALYELFIQGLMPERFAVVATGRSKVDDEVYRN
                     EVSSALTGKAGKEGGKGDDDFMRAFFYTTLDPLVPGDYRELAGLLNRLRSEMKIQGNT
                     LFYLSVPPEIYDEISPGLAGQGLNSEADGWKRLIIEKPFGYDYASAMHLNRLLLEHWK
                     ESQLFRIDHYLGKETVQNILVTRFSNGIFEPLWNRNYVHHVEITSSESLGVERRGSYY
                     EGTGALRDMVQNHLLHLVGFIAMEPPSSIDSTSIRNELVKVFESLRPVKPEDVGKMTI
                     RGQYTGSHVKGEWIGGYREEESVSKTSMTETFAALKFHIDNWRWGGVPFYIRTGKRLP
                     TRVTEAVIHFKPTPHYLFSGESACEGCNQLIIRIQPDEGILIKFGMKIPGGGFKVRNV
                     NMDFHYSDLAVDRIPDAYERLIHDCMNGDSTLYTRSDAVLAAWKFLDPVLQAWKDDPS
                     IPLYGYPAGTWGPETADGLIEEPELTWRYPCKNLADDGVYCEL"
     gene            9181..9912
                     /gene="pgl"
                     /locus_tag="PKK03_08985"
     CDS             9181..9912
                     /gene="pgl"
                     /locus_tag="PKK03_08985"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNX84575.1"
                     /translation="MSHSVRVFPTREALAQSIAAMLIKDLDRIEPDHYYTIALSGGST
                     PEYIFRYLSENYAERISWEKLLVFWVDERCVSPDDDESNYRMAYESLLQNVPIPDANI
                     FRIRGEDDPVAEARQYSELVTSMVPEYRGIPQFDLMLLGLGSDGHIASIFPDRPDLFT
                     TGQLFEVAARPDKKQKRITATGRLINNSSKIYYIVTGKEKAPVVSQVLEGRSGSELYP
                     AARIKPGSGHLVWMLDTGAASLLKG"
     gene            9941..11077
                     /locus_tag="PKK03_08990"
     CDS             9941..11077
                     /locus_tag="PKK03_08990"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HNX84576.1"
                     /translation="MIAGILRETGGEKRVALLPGETAALVKLGLKVLVETGAGEEAFA
                     SDDQYLAAGAAVAERSGVIRESGLLLTVNPPVSEDITLFREGQIIFTALDPVSNREWL
                     EKARTGKLSIMALDLVPRITRAQSMDILSSMATVSGYKAVLEAASMLPRFFPMFMSAA
                     GTIKPARVLILGAGVAGLQAIAVARKLGAVVEVFDVRSAVKDEVKSLGGKFIEVEGAR
                     EDATAGGYAVEQSEEFRKKQQALIQERAAAADVIIATAQIPGRKAPVLILKETVGKMK
                     PGSVIIDLAASSGGNCELTENGRTVIISGVTIAGRSDYPSMMSADASRMFGNNLVNLL
                     RIMTDNKGEVRIDLADEIIAGTAAVHDGEYRSQRVKQMLDIKLT"
     gene            11080..11382
                     /locus_tag="PKK03_08995"
     CDS             11080..11382
                     /locus_tag="PKK03_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNX84577.1"
                     /translation="MEGILEFFMANRGTIYMVILSVFLGIEVISHVPAILHTPLMSGA
                     NAIHGVVIIGAIIVMGHADTTGAMILGFLAVVLGTLNVVGGFVVTDRMLEMFKKKN"
BASE COUNT         2799 a         2687 c         2941 g         2931 t
ORIGIN      
        1 tgatctttcc atccggtttg ctttggaaca aatttatggc taaataataa gttcatggca
       61 aattcgttta ttaaaaaagt gtaaattggt ctgaagaagg cctgtgaaat cataaactac
      121 aaatactatg aacagtaagc ttaagattct gccactgatt ctgggcgtaa ccgctctggc
      181 attctgcctg gctgccacct catgcaaggg aaaaaccagg ggtgaggcaa acaagggcca
      241 ggaggaggcc gtcagtcctg atgagcagac gatggagcag ataaagagtg ccgagaaggt
      301 attcaatgcg ctgcccacac cgctcgaggt ggctcagctg ataaaggagg caggggcgaa
      361 gttcgatgct acactgctga atcctgttga gaacaagagc aactacacct ccagcaaaag
      421 tatggcgctg aacctgggca catatgtttg tgacctcagt tttgccagca tgtatgatga
      481 gacttcattg ctggtcaatt atatggaggc tgctgaggac atggccacag gccttggcat
      541 tctcgacgcc atcgacgaga ccaccattga gcgccttgag cagaatatca ataaccgcga
      601 cgttatcatg gagatagtct cggagacatt cctcaattca caggcttatc ttgaggatga
      661 tgagcaacat gccgtggccg ctacaattat tgcgggaggc ttcatcgaag ggctgtacat
      721 atcgaccagc ctggttgata tgaagaacta caagggcaac aagctggtcg gaacaattgc
      781 tgatcagaag atgtcagttg atgacatgat acgccttctg gaaacctata ctgacaatac
      841 tgcaatacgg gaactcctcg ggcccataca cgaactgaag gccatcttcg acaggattga
      901 aaggtcaacc acaccctcaa ctgcccagta caatgaaaag atcggcatga cggagatcac
      961 cggtgcggca aaatcggaga tgaagcagga ggttttcctg gcaatcaagg ataaggtggc
     1021 agagatcaga aattcatatg taaaatagat accgatgaga cagcttatac agattttact
     1081 ggttacggtc ctcctttccc tccttgttcc cggggaagct gccgcgcagt gcaagggatt
     1141 tgccaaatca ctatgcaaga aggaactggg cgcctacctt catgacggta actaccatgc
     1201 agccgtcctg gttgaaggtg aagaggctga actctataag acattctact ccgacctgga
     1261 gtaccggctt gcaatttgtt cggctgacgg tatgcctccg gtggagttca cagtttatga
     1321 tgccaccggt aaagtgctct acaacaataa ggatcagggt ctggccaaaa catgggactt
     1381 caggctcgag gcaagccagc aactgaaagt ggtcgtaaaa gtgtcaacct tcggtacaag
     1441 agaacaattg ccggtcagcg gttgcgtggc aatcatgttc ggtttcaagg caaagtagga
     1501 ttgctcaggt aaattgaccg gcaggagggg cagagagctg cgcccttcag gtcaatctcc
     1561 tcggcatagg tgctggagcg catcacacag ctgaggttat ggcagtggac cagcccgaag
     1621 gtatgcccaa gttcatgtat tacttcctta atggttcttg acaggagaat gtcatcatcc
     1681 tgtttaagtc cgtacagttc gttacgaaga cggaagaccg aagctatggc taattgccgg
     1741 cccaggattg cctgtccgaa aatgtaggta agtatgggga tgtaaagatc aaccctgaaa
     1801 agcccgataa ttttgaaata ttcagaggtg gtgcaggccg gaatctggtt cagcaacctg
     1861 tctccgtcat actgtcttcg ggccggattg taataggggc tgagatcaag gtggcactcc
     1921 tcggttatga cctgacacct gtattcatct tccactgcgc cggagacctt tttcaggaac
     1981 cccctgtcaa aggagccgca ggaaagcaat atgatctttt tatcttccaa tgggcgggga
     2041 tgagtgacag ttactccttc tgtctcaggt catatttttt aattttgtta tagagagtca
     2101 ccctgtcaac actgagcgcc ctggctgaac gggatatatt ccagttgtat ttcagaagta
     2161 tttgctctat gtgcctcttt tccagttcgt caagactctc cacggaattt tcgggtgttt
     2221 ctgcccccag cggaaggtcc ttgagcttaa tctccctgcc gttgccaatt acgatggcac
     2281 gctcaatcat gttctccagt tccctgacgt tacccggaaa ttcaaatttt tcaagatgtt
     2341 tcagcgctga cggctcaatg gtgatgagat cacggctcat tgaagtgcag tatttcctga
     2401 tgaagtggtt gaccagcagc ggaatgtcgt cagttctctc tctcagggga ggaatgtcaa
     2461 tgttgaccac attaagcctg tagtagaggt cctcacggaa tgtaccgttt ctgaccatgg
     2521 ctgccaggtc acggttggtt gcacatatga ctctgaagtc agaagtaatt tccctgttgc
     2581 cacctaccct gacaaagctt ttcgtctcaa gcacacggag cagctctatc tgcatcttcg
     2641 gggagatagt ggcaatctca tcgaggaaga tcgtacctcc gtcagccatc tcgaagcgtc
     2701 ctttccggtt gaaggtggcg cctgtgaaag cgcctttctc gtgtccgaaa agctcgcttt
     2761 caagcaggct ttcggtcagg gctccgcaat ggacacttat catgggaaag tattttctgg
     2821 aggagttggc atgtatggct ctggcaacca attccttgcc ggtgccgctc tcacctgtaa
     2881 taatgactga ggcatttgac tgggctacac tctctatatg ctgcaatacc ctgactattg
     2941 cttcactgtg gccgatgatg tcatcaacct cctccagggt agtgaccctt tccttaagcg
     3001 cctcattctc atttctaagc gctatctgac ttgctgcatt ccggatgagg tgcgacagat
     3061 catcaggatc gaacggtttt gtcacatagt caaaggcacc gtctttcagt gcctgtaccg
     3121 ctgtctcaac agaggcgaag gctgtcatta ttattacgat agtctcgcgg ttcagcgatc
     3181 tgatccttct gtgcagttca agcccgtcca ttcccggcat cttgatgtcg gcaaggatta
     3241 tgttgaaatt tctctcttcg agaagctgca gtgccttttt tgcattctcg gcggcttcca
     3301 cctcataacc gtcctcaaga aaccagttaa gcagggaatc ccttacagag tcctcgtcgt
     3361 caacaatcag aattgaaatc ttatctgcca tacccggtgg tttattctgt tttcaatggt
     3421 aaaataattg agatggtggt tcccctgccc actgccgaat caacctcgat cctgccgttg
     3481 tggttctgca caatgccgtg cactatcgag agccccagtc caatgccgga tgcatcctgc
     3541 ttggtggtga agaagggttc gaatatgtgg ggtatgtctt caggggctat gcctgacccg
     3601 ttgtcggata tttcaatccg aaccgtggtg ctgtcgctgt tcctggtcct gattgaaatc
     3661 tccccgtttt cggtaacagc ctccgaggca ttgaccagga gggcgacaca ggcctgcttg
     3721 atctggtttg cgctgcatcg gaccaggtca gcctttgctt ctgtcataac ggtgaacctt
     3781 atattggaga tcttcacgtg atgcagcatc agctcatagg tgccttccac tatttcattc
     3841 agatgccttt cctcgaagtc ctcctggtcc ttgcgtgaaa agtcaagtaa acccttaaca
     3901 atgtctccgc acctttttgt ttcagtctca ataagcttca gctggcgaag gatattctgt
     3961 tttttggccg cagtcaggtc ctggctttcc acctgtttgt gcacgagctt ggtatagatc
     4021 aggatgcctg aaagcgggtt gttgatctcg tgtgcaacag atgctgacag cttgcccagg
     4081 gacgcgatac gctcgacatt catcagctcg ttgtgtgctt cggagagttc ttctgacttt
     4141 ttctgaacct tgtactcgag ttgttttgac cagttttcaa gttctctcgt tgcattgtca
     4201 atatggtcga gcatgttatt gaaagccatg gagaccatct tcatgtcatc cagcaggttg
     4261 ggtttgatgt cgagccttgt gctcacctca ccctgcgaaa cagcctcgct ggcactgatg
     4321 atgcccagaa gcggggcttt gatcctcttc ctggtgaacc agatgaggat cattacgata
     4381 attccggata tgagcagagc catcaggagg aattgcacgg aagatttccg cagggcgctg
     4441 tcaagatcac ggaggggcat cctgatgatg agagacccca ggacttcgtc gtccctgctg
     4501 tgagcatggc atgcggcagt gtagcacgaa agctcgttga gtatgggcct gcgtataagc
     4561 atatgcctct ctccgggttt gttggggttc attgcacagt cgctcttcgc atcaattatc
     4621 atgtagctcc tctcattcct tgaaaacatc tgtccgaggt cagtatggca cgagatgcaa
     4681 tcggggttgc tatgattccg ggcgtcagca gcaaatgacg aataggccag gttgtcattc
     4741 tggtcataca ggttcacgtc atcaataccg ggcatggtgt taatgatgtc aagagtacca
     4801 tgaagggtgc tcttgtcatt ctccagcatc gatttataca gagagccctc tactatagca
     4861 cctacattat tcccgttctg tttcagaacg gtgttgaaat agttgatata aaccgtgcgg
     4921 aaaattgcgc tgaacgatat gaacaataca actgcagcga gggtgattat ggtcacaacc
     4981 ctgccgtaaa tggttgaact gaatctgaaa taccttcgca tcttttctcc cagggattcc
     5041 cgcgggctgt ccgtttttcc tcgttgagcc atttttatcc tagttgggtg caaaacaaac
     5101 taatgtgaac gcccacgaca gttgtggacg atcacatatt gtgtttaaat tttctacaaa
     5161 atacgaaatt aaagtattca ggacaagtga ggctgcactt ttcaggacct gttgatgaat
     5221 ctgatctgga actcttccca gacttcaggt ccgaactcct cgaggaagcc ttccttcagt
     5281 tctccgccgt cctgaagcac cacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnngtccgaa ctcctcgagg aagccttcct tcagttctcc gccgtcctga agcaccacat
     5461 gggccagtct catgtcgttt gcaccggcca cagtggcaag gaagtcgcgt atgcggccga
     5521 actcttcgcg aagttgaact gcaaatttgt ctgcggcagc catcaggcgt gattcattga
     5581 accagttatc aggttctatg ccgcacatcc atccttccga gtagggcccg ctgtgaagca
     5641 ggtcagtatt gtttgccagg gtctcattgc gtgagaccac tgttccgctg acgggtgaga
     5701 ggacgtcaag ctgcttgcct ttctgaatga gtgatgctat cctttcgccc ttgcgtacct
     5761 tttcaccagt tgctttcagt gtgatttttg tcacgatacc ggttacgtgc tgaaggaagt
     5821 cgtcaatacc gactgttacc gtgccgttct tttccatgaa ggcccaggtg tggcttttgt
     5881 cgaataggat tcctgcaggt gtcacaaagg ttttagggtt gaatgctttc agcggttctt
     5941 cctccaggag gctgatcttt cctttgccgg ccctggtgat gcgatacaac aggaagccga
     6001 ggagtgagat ggcggagatt actgccatta cccataccag gatgctgacg cctgccttgt
     6061 tttcgttgcc tgcctgcact atctccgtca caggtgtcag catctccctt ctgatcattc
     6121 cttcacttcc cgaaagggca gtcaacccgg aggtggctgc tatctcctgg ccgtcagcca
     6181 gaatccagcg gatgaaatca gcagcggctt cgggtaccat ctgcgaggcc gtggcacaga
     6241 agatactgtt gcagagctcc ttcggatact tgccgatgta tacgccacgg ttgaagctgt
     6301 tgaaatctgc ataaaactgc tcaaaatagt ctgactgacc gttggaattg atgtcaatcg
     6361 gaactaccat caggttttca gcaaaaccgg taccaccggg agctgttatg tcagacagtt
     6421 tgcagaaaac gacagtggca ggctgattcc tgagcagcga tgctatctca gagactggca
     6481 attcccgtgc aatgacctgc gaagcgtcga tcccggcaaa tcctgccaga taacccagag
     6541 ctgctccgtc aacagcaaca tttgtcattg cagggacatt ggcttccgta cccagaatct
     6601 tgccccaggt gactgtaggg gaggagagaa gcgcaccaaa cttttcgggg gcgatgcctt
     6661 tcgacattat gacctggtag aatggattgt cagtactcat aacaggaact atcacatcac
     6721 gcgcaacaac cattttccat tccgattcgc cgctggctgt ccctgcggag gcaccttcaa
     6781 atatcctgat atcagctttg ttatactcgt caactggcag catcgacaca ttccggccgg
     6841 gattttccac cctgtaggcg tcaatccagc tctgagccag ggctttaagc cccgttgagg
     6901 aagctactgt gacggcatcg gtgtttttgc cgtcctgctc cggccctgca tactgcgagc
     6961 ctgataatcc tgcgctcagg caggcgaggc tgataataaa gatgattgac tttttcatat
     7021 agttgtattt tatattgtct gtaataatgt ataccaataa gcatgccaaa gcttgtaata
     7081 tattgagaac caataatata aagcactttg aggtctgtat tgttcctaca aagtgttgat
     7141 aaattaaaca ggcaataatg gctattgata taaagcacat agaatcagtc tgataagaca
     7201 atgtgtaaat aatatacata tgtataaaaa tgcaacactt tgaagaatta acatgtaagg
     7261 tctcaggacc gggagcttca atgtaatgat ttgttcaata aacttttaaa cagtatcagt
     7321 tgttttagat gatgatgatg tcagagaagg gctcaatttt ctatatttgt tttaactgtt
     7381 gttgcaaatg caaggattga acatttatgt gttgatcccg ttgaccgggg caattatgaa
     7441 tgatgtctgc ggaaggctgt aaacgaagct gatgaacagg gcggagaacc agataattgt
     7501 gatattcgga ggcaggggag accttgccat gaggaagatt gttcctgctc tgtatgagct
     7561 ttttannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggcag gggagacctt
     7681 gccatgagga agattgttcc tgctctgtat gagcttttta tacagggact gatgccggag
     7741 aggtttgcgg tggtggccac aggaaggagc aaggttgatg atgaggtcta cagaaatgag
     7801 gtgagtagtg cgctcacagg gaaggccggg aaagaagggg gaaaaggcga tgatgatttt
     7861 atgcgggctt tcttctatac gactcttgat ccgttggttc cgggtgatta cagggagctt
     7921 gcagggcttc ttaacaggtt aaggtctgag atgaaaattc agggcaacac cctgttttat
     7981 ctctccgttc cgccagagat atatgacgag atttctcctg gtcttgccgg tcagggtctg
     8041 aacagcgagg ctgatggctg gaagcgtctg attatcgaaa agcctttcgg gtatgattat
     8101 gccagtgcta tgcatctgaa caggttgttg ctggagcact ggaaagagag ccagcttttc
     8161 cgcattgacc attatcttgg aaaggagact gttcagaaca tacttgtgac ccgcttttcc
     8221 aatggcattt ttgaacctct gtggaacagg aattatgttc atcatgtcga gatcacatct
     8281 tccgagagcc tgggggtgga gaggaggggc tcgtattacg aaggcaccgg tgcattgcgt
     8341 gacatggtcc agaaccatct cctgcacctt gtcggtttta ttgcaatgga acccccgtca
     8401 tccattgact caacatcaat caggaatgag ctggtcaagg tctttgagtc actccggccc
     8461 gttaaaccgg aagatgtagg aaaaatgacc atcaggggtc agtacaccgg atcgcatgtc
     8521 aaaggtgaat ggattggcgg ttaccgggaa gaggagagtg tcagcaagac atcaatgacc
     8581 gagacatttg cagcgctgaa attccatata gacaactgga ggtggggagg ggttcccttt
     8641 tacatcagga caggcaagcg tcttcccacc agggttacgg aggctgtgat ccatttcaag
     8701 ccaacacctc attacctgtt ctcgggagag agtgcgtgcg agggatgcaa tcagctgata
     8761 atccgtattc agcctgatga agggatactg atcaagtttg ggatgaagat cccgggagga
     8821 ggattcaagg taagaaacgt gaacatggat ttccattact ccgatcttgc cgtggaccgc
     8881 atacctgatg cctatgagcg gctgatccat gactgcatga atggtgactc aacgctttac
     8941 accaggtctg atgccgtact ggcagcatgg aaattccttg atccggtatt gcaggcatgg
     9001 aaagatgacc cttctatccc gctgtatggt tatcctgctg gaacctgggg accagagacg
     9061 gctgatggcc tgattgaaga accggagctg acatggcgtt atccgtgtaa aaatcttgca
     9121 gatgatggtg tttattgtga attataatta gttttatatc aagattactg tttatttgtc
     9181 atgtcacatt cagtcagagt gttccccacc cgtgaggcac tggctcaaag catcgcggcg
     9241 atgctgatca aggatctcga tcgtatagag cctgatcact attatacaat tgctctctcc
     9301 ggaggctcta ccccggaata catattccgt tatctttcag aaaactatgc agaacgaata
     9361 agctgggaaa agctgctggt cttctgggtt gacgagagat gtgtctcccc tgatgacgat
     9421 gaaagcaatt acaggatggc ttatgaaagc ctcctgcaga acgtcccgat acccgatgct
     9481 aacatcttta ggatccgtgg cgaggatgac cctgttgcag aggcccggca atattcagag
     9541 cttgtgacca gcatggtgcc ggaatacagg ggcatcccgc agtttgacct gatgctgctc
     9601 ggtctgggca gcgatggtca catagcttcc atctttcctg acaggccgga cctgttcact
     9661 acaggtcagc tcttcgaggt ggccgccagg cccgataaga aacaaaagag aattacagcc
     9721 acgggccggc tgataaacaa ttcatcaaaa atatattaca ttgttaccgg taaggagaag
     9781 gcaccggtcg tatcacaggt tctcgaaggg aggagcggct cggaactcta tccggcagcc
     9841 aggataaagc ccggctcggg gcatctggta tggatgcttg ataccggagc tgcctcactc
     9901 ctgaagggct gaaaaataaa caatatgtaa cattccggct atgatcgcag gtattctcag
     9961 agaaacagga ggtgaaaaaa gggtcgccct cctcccgggc gaaacggctg cattggtcaa
    10021 actgggactg aaggtcctcg ttgagaccgg cgccggcgaa gaagcctttg cttctgatga
    10081 ccagtacctt gctgccggcg ctgccgtggc agaaaggagc ggggtgatca gggagtccgg
    10141 cctcctactg acagtcaatc ctcccgtcag tgaagatatc accctcttca gggaagggca
    10201 gataatcttt actgctcttg atcctgtttc caacagggag tggcttgaga aagcacgtac
    10261 aggaaaactt tctatcatgg ctcttgacct ggtgccacgc atcacccggg cacagtcaat
    10321 ggacatcctc tcatcaatgg caactgtttc aggatacaaa gccgtgcttg aggcagcttc
    10381 catgctgccc aggtttttcc ccatgttcat gtccgctgcc ggcaccataa aacctgccag
    10441 ggtgctcatc cttggcgcag gtgtggcggg attgcaggct attgcagtgg cgcgcaagct
    10501 gggtgcagtg gtggaggtct ttgacgtcag gtcagccgtg aaagacgagg tgaaaagcct
    10561 cggcggaaaa tttattgagg tagagggagc cagagaagat gctacggcag gagggtacgc
    10621 tgtggaacag agtgaggagt tccggaagaa acaacaggca cttattcagg agcgtgctgc
    10681 tgctgccgat gtgatcattg ctactgcaca aatacccggc agaaaggccc ccgtgcttat
    10741 acttaaggaa acagtcggga aaatgaagcc gggctccgtg attatcgacc tggccgcttc
    10801 ctccggtgga aactgcgagc ttactgagaa cggcaggact gtgataataa gcggtgtaac
    10861 catcgcaggg agatctgact acccatccat gatgtcagcc gacgccagca ggatgttcgg
    10921 aaacaacctg gtcaacctgc tcaggatcat gactgacaac aagggagagg tgagaattga
    10981 tctcgccgat gagattatag ccggtaccgc tgccgttcat gacggcgaat accgcagcca
    11041 gagagtgaaa cagatgcttg atatcaaact gacctgatta tggaaggaat acttgaattc
    11101 ttcatggcca acaggggaac aatctatatg gtgatcctct cggtctttct tggaatcgag
    11161 gtgattagcc acgtcccggc catactgcat acaccgctta tgtccggggc aaacgccatt
    11221 catggtgtag tcatcatagg cgcaatcatc gttatgggac atgcagacac aaccggggca
    11281 atgatactcg gtttcctggc agtggtactg ggcacactga atgttgtggg cggtttcgtg
    11341 gttactgaca ggatgcttga gatgttcaaa aagaaaaatt gatacgatat agaccctgac
    11401 cctgataaac gatggcacaa tgaacgacgt atcacatttt tctgccggaa tctccgttct
    11461 cgagatctca tatatactgg cttcggtatt attcatcctt ggcctgaaga tgctcagtca
    11521 tccactgtcg gcacgcaggg gaaaccttat cgctgcgg
//
LOCUS       DAOPXU010000086        11356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_6524_length_11356_cov_14.366074, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000086 DAOPXU010000000
VERSION     DAOPXU010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11356
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6524_length_11356_cov_14.366074"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PKK03_09000"
     CDS             <1..315
                     /locus_tag="PKK03_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84578.1"
                     /translation="VHHANPAISAVTVDMLTEKDTLSRIWNKHGAYVETEEMKIKEIV
                     PDSVLKFKGDKIKMMQKEIRKAMAEAAGDDDKISGLQEQYKAMTSLQKKISAGLGGRL
                     II"
     gene            503..1405
                     /locus_tag="PKK03_09005"
     CDS             503..1405
                     /locus_tag="PKK03_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84579.1"
                     /translation="MRHFLTVTVATCAVILLTASCNRTDKGIKAPAPVFSTYIPESPG
                     DTTRIREIYYGLMTPVEVCNIFERLGIHFNDTIILPPENRDLYMSSYKAAMNLGVYGV
                     DMGYVKLFGVNRQTMSYFNTLKTLSNRLSMPDSFVSDAIKSLDPEMSNPDSVTRIMND
                     VYSKIDHHLREEGNEGTLGLMLMGGWIESMYLATQLAYDPANPDPQVVGKIAEQKYSL
                     ISLLSFMKNYYSDPMVVFYTKKLKYLNRWFDTFDIYYQPGDITVDTLRQVITTTGTEM
                     TVTVDTLDEIRDYVAKLRAEITAV"
     gene            1487..7051
                     /locus_tag="PKK03_09010"
     CDS             1487..7051
                     /locus_tag="PKK03_09010"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNX84580.1"
                     /translation="MNRRLLQVTAVIAVISMSCGQGKKESGASSSGAVSPAVTVIDKG
                     FSKFISAYTSGIVPVNGTLQVVFTPEFAATVDRTRTQGLFSFTPALKGSAEWADDITL
                     VFKPSKLLAPGTAYQGTLDMSRLGAVEERFRFFPLAFRTVEKNFTVSLNPVTVDLPDG
                     NTYTLTGTLVTSDQIDNAEVEKYLTVSVGKRNESIVWDHSGGNVHNFSVEKIRRAKEK
                     SELTVTWNGNAYGIKSKGEMAVTIPAEGVFTVTDVKISAGESKSIEIFFSDLLDAAGE
                     PDGMVTADPSFPMTISAEANRLLVIPGENVSGVVELTVDGSLRNTRGERLGEPVKRSI
                     NFTPVEPGIKSAGRGVIMPSSGEIIFPFLAANLSAVDLTIIKIFENNLPYFLQQNNMG
                     EANYYQNSITQFGRPVYRGRVDLTGSAGFDPNGYNLFTIDLANYIEPEQGILYRIELG
                     MRPSYSLYPCTGERKHSKYEEMLELADNDRRWEGSDDYYSFSEEGLFYEYAYNWREYE
                     NPCSDAFYSPDKKLVRNILASDLGIIAKSGPDNNLRIFVRDLLTADPVEGATVEAYDF
                     QNQLMGSATTDRSGIAAIPCPRKPFLVIARKDKNRNYLSLLDGNALSMSSFDVSGESP
                     QDGIKAFLFTERDVRRPGDSIYVGVIVRDLGKGLPAGHPVHFELYNSKGQRIDDQVTT
                     LNDRGFLAFTTVTADDAETGNYRAQVRIGAATFTKVIKVETVKPNRLKIKLDFPDAVA
                     GGDDNTINGNMKVTWLNGSTARDMKSTVEMLLKPVKTSFERYRQYNFDDPSAQFYFES
                     QTIFDGDINSSGEASVSFSPDEQMQAPGMLNAIFTTRVFEKGGDASITRTVVPYAPYP
                     AFVGMNIPALGAAGRIIYTDRPNEVRLVTLDKNGKPVRSEVEINVYKLDYRWWWESDD
                     EYLGSYISGERNANIFSETITTSGGEGKTSFSVGKEDWGRYLVRATLPSGHSTGIIVL
                     VDWPWDYGMKPGGNDGASLLQLNTDREKYSVGDEISLTFPAPENGRAIVTIENATGLL
                     DVTEVKTRAGNTEVKIKATPAMAPNAYLYVTMLQPHSQTVNDAPVRLYGVIPVMVEDP
                     ATKLVPVITMPDKIRSQQEVEIRVEEKNRQEMTYTLAVVDDGLLDLTGFRTPDPWKWF
                     FVKNALGVKTWDLYDIVFGAFGGKLGRGFAIGGSEAAIDQSKNRERRFEPVVRFIGPF
                     TLAPGKTASHKIRLPQYTGSVRVMVTAAGKANSFGAAEKTVTVADPLMILATAPRVLS
                     PGDKVALPVTVFAQDKKISGVSVSATANDMITMTESAGAVSFSEPGDKDLELMFSTAT
                     RTGTAKISVSAEGSGEKASYDLTVPVRSPNPPDRRAETKSVAKGQKFEKSFSPFGMAG
                     TSAVSVEIFTLPSVNLSKRLKYLTSFPHGCSEQITSAAFPQVYLPDLLGSGLDNPAVV
                     RNNVQEALRALSTRQMASGAVTLWPGGTYPDDWVTSYVGHFAVEAQKAGYAIPAGFIR
                     KWTSYQRKQASAWRYQPQYRYTANDQAYRIFTLALAGSPDKGAMNRMREITDLPALAR
                     WFLAAAYATTGRTEVAQTLIDVRNLKTEDDYVNYYYGSSLRDQSIILYTLTQMGNTGE
                     SIELLKDVAEALSHDTWYSTQTTAWGLFAYMNYAKMMKSDASKPLKATLEFNGNREKL
                     STDGGAIMKNFTPAASNRLVVENESENPLYVTVTEEGVPSVADMTVKENNLAMKVSYF
                     NMESEPVNVSSLAQGTSFIMAVDVTNTTFRPVVNLALTQMVPSGWEIQNTRLFDAGLQ
                     LKEGAYDYRDYRDDRVYTYFSLRSGETKKFFIILTASYRGSYSMPAVVCEALYDESYA
                     ARRPGFGVEVKK"
     assembly_gap    7170..7269
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7338..9701
                     /gene="pbpC"
                     /locus_tag="PKK03_09015"
     CDS             7338..9701
                     /gene="pbpC"
                     /locus_tag="PKK03_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HNX84581.1"
                     /translation="MRSVFGRRRSPDSRRLSGALRIVIPGLAALLFAILVVPMPSFRA
                     PLSTVVEAADGTLLGARVAADGQWRFPPPDSLPDKYITCLINYEDRWFRWHPGVNPVA
                     VTKALTDNIRAGETVRGGSTITMQVARMARGNPERTYGGKIIEMLSAVKLELFRSKGT
                     ILKLYAANAPFGGNTVGIEAAAWRYTGRGTADLTWAEAATLAVLPNAPALIYPGRNSE
                     RLKEKRDRLLQTLAERGKIDSLTCELAMGEPLPGAPLQMPSLAPHLTGRLWIEEPGTR
                     KRTNVDPLLQREASELVDRHVSALEGNGIHNAAAVVVEVSTGNVVAWVGNSTLPDTTG
                     RHGRDVDMVTAPRSTGSIMKPFLYAGLLSSGMLLPNALIPDIPTRFQGFRPENADFSY
                     SGAVPAGDALARSLNIPAVRMLQMYEEERFLLLLRQMGFTTFTKPASHYGLSLILGGG
                     EATLWELASAYASMARVLITNPGTQTSNSSSSIPNRKSQIAIPPLSPSSIWLTYEALR
                     RVNRPETETGWQYFGSAPEVAWKTGTSFGFRDAWAIGTTPQYIVAVWAGNADGEGRPG
                     LSGIASAAPLLFDIIRLLPPSASWFAKPDDGMTLVEVCAASGYRAGPDCTDRKDEWVP
                     EAGLRSEACPYHTIVSLDPTETWRVNSSCAAPGEMVARSWFVLPPAMEYYYRMRNPSY
                     RTLPPFRQGCSDDMKLPSMEFIYPSRDARIFIPRSLQGQLMSMLPEIAHRRRNATVYW
                     HLDNQYIGMTRHIHQTEIRVGEGEHLITAVDEEGMTVSRKFYCIGTL"
     gene            9762..11285
                     /locus_tag="PKK03_09020"
     CDS             9762..11285
                     /locus_tag="PKK03_09020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX84582.1"
                     /translation="MTTLSFGSFFKRIIAGTAFNRYRQVFDRAGYAIMLIDNATNHFI
                     KANRAASELFGYTEEEFRKMTPLDLSTETGEEPTLPGEKTEESLIRPARRKDGKIITI
                     KVQTSILERFRISLISDVTEEYRIRRALELNEERLREAQRIGRFGWWEYRPETNRYIG
                     SEEISRIYNDEPSNLSFTYQDNISYTHPEDRSYLITAIEDALEKKKESYSLTYRIITP
                     NGKVKAIAVNSNVRYDENGRLLYRYGTCQDITETQKIMDDLIAARRQAEESDRLKTTF
                     LANFSHEIRTPLNAMLGFLNILTSMDTTEKEKEEMVKLIEINSQRLLRLINDMIDMSR
                     IESNSMEIKYTNIEINGFVNSMVSSFNHELEASGKKDLRLVPVVDKTKAAVYQYIDEL
                     RLSQIFSNLVSNAIKFTDQGEIKYGYRISRTDNRYSIVYFVTDTGQGIRPDRLDDIFL
                     PFVQYWNEDTPKVQGAGLGLAICKRLAELMKGRIWVESLPGKGSSFLFSFNTPVMIE"
BASE COUNT         2711 a         3155 c         3100 g         2290 t
ORIGIN      
        1 gtgcatcatg ccaatcccgc catcagcgcc gtcaccgttg acatgctgac ggaaaaggac
       61 accctcagcc gcatctggaa caaacacggt gcatacgtcg agactgaaga gatgaaaata
      121 aaggagatcg ttcccgattc ggtcctcaag ttcaagggag acaagatcaa gatgatgcag
      181 aaggagatac gcaaagcgat ggcggaggcg gccggtgatg atgataagat atccgggttg
      241 caggagcagt acaaggcgat gacctcgctg cagaagaaga tctccgccgg cctgggcggg
      301 aggctgatta tctgatcacg cgtgctacaa tgcctgcccc ccccgacaac ttccggctat
      361 cgggaccgcg tccacgagga tgatcggcaa tcgcaaatca cagatcgcac atctaaaatg
      421 cctgttggtc tgggtcaaca tcgcaacatc gcaaatcgca aatcgcaact ttttattacc
      481 ttgtggcaaa tttctgcata caatgagaca tttcctgact gtgaccgttg ccacatgcgc
      541 cgtgattctg ctcaccgcat catgcaacag gacagacaag ggcatcaaag cgccggcgcc
      601 ggtcttttct acatacatcc cggaatctcc gggtgacacc acacgcataa gagaaatcta
      661 ttacggcctc atgacacccg tggaggtatg caacatcttc gagagactgg gcatccactt
      721 caatgacaca atcatcctcc cgcccgagaa ccgcgatctc tacatgagca gctacaaggc
      781 ggcaatgaac ctcggcgtat acggagtaga catgggctat gtcaagctct tcggcgtcaa
      841 caggcagaca atgagctatt tcaacaccct gaagacactc agcaaccgcc tcagcatgcc
      901 cgactccttc gtctccgatg ccataaaaag cctcgatccg gaaatgagca atcccgactc
      961 ggtgacacgg attatgaacg atgtttacag caagattgac caccacctcc gcgaggaagg
     1021 caacgagggg accctcggac tgatgctcat gggaggatgg atagagtcga tgtacctcgc
     1081 cacgcagctg gcttatgacc ccgcgaatcc tgatccgcag gtggtgggca agatcgccga
     1141 acagaaatac tctctgatct ccctgctgag cttcatgaaa aactattaca gtgacccgat
     1201 ggtcgttttt tacaccaaga aactgaagta cctcaatcgc tggtttgaca ctttcgatat
     1261 ttattatcag ccgggagaca tcaccgttga caccctccgg caggtgatca ccaccacggg
     1321 tacggagatg accgtcaccg tggataccct ggatgagata cgtgattatg tggcgaagct
     1381 cagggccgag atcactgctg tctgaatcat ctgagtgaga tcaccggaac catgtgagcc
     1441 tgaaagtgag tactgctgtc tgaatgaacc tgtaataaaa tatactatga accgtcgtct
     1501 tctccaggtc acggctgtta ttgccgtcat ttcaatgtca tgcgggcagg gaaagaaaga
     1561 gtccggcgcc agcagctccg gggcagtctc tcctgccgtc accgtcattg acaagggatt
     1621 cagtaaattc atctccgcct acacttcagg catcgttccg gtgaacggca cgctccaggt
     1681 agtatttacc ccggagtttg cagccaccgt tgaccgtacg cgcacacagg ggctcttctc
     1741 attcacgcct gcgttgaagg gatcagcgga gtgggctgat gacatcacac tggtattcaa
     1801 gccgtcaaag ctgctggcgc cgggcaccgc ctaccagggc acgcttgaca tgagcaggct
     1861 gggcgccgtg gaggagcgct tccgcttctt tcccctggcc ttccgcaccg tggagaagaa
     1921 cttcaccgta agcctcaatc ccgttaccgt cgaccttcct gacggaaaca cctacaccct
     1981 aaccggcacc ctcgtcacct ctgaccagat tgataatgcc gaggtggaga agtacctcac
     2041 cgtcagcgtc gggaagcgca atgaatccat tgtctgggac cactcgggag gaaacgtcca
     2101 taacttttca gttgaaaaga tacgcagagc gaaggagaag agtgagctca ccgtgacctg
     2161 gaacggaaac gcatatggta taaagtctaa gggcgaaatg gctgtaacga tacccgccga
     2221 aggtgtcttc accgtcaccg atgtgaaaat cagcgccggg gagagcaaga gcattgagat
     2281 tttcttttcc gaccttcttg acgctgccgg tgagcccgac gggatggtga ccgccgatcc
     2341 gtcgttcccc atgaccatca gcgccgaagc caacaggctc ctggtgattc ccggtgaaaa
     2401 tgtgagcggc gtcgttgaac tgaccgttga cggctctctc cgcaacaccc gcggtgaacg
     2461 gctcggagaa cctgtgaagc gcagcattaa cttcaccccg gtggaaccgg gtataaaatc
     2521 agccggccgg ggcgtcatca tgccctcgtc gggagaaatt atcttcccct tcctggccgc
     2581 caacctgagc gccgttgacc tcaccatcat aaagatcttc gagaacaacc tcccgtactt
     2641 cctccagcag aacaacatgg gtgaggccaa ctactaccag aacagcatca cgcagttcgg
     2701 caggccggtc taccgcggac gcgttgacct cacaggcagc gcaggcttcg atcccaacgg
     2761 atataacctc tttacaatcg acctcgccaa ctatatcgag ccggaacagg gtattctcta
     2821 ccggatagag ctcggcatgc gtccctcata ttcactctac ccatgtacgg gcgaacgtaa
     2881 acactcaaaa tacgaggaga tgcttgaact ggccgacaat gaccgccgct gggaaggttc
     2941 tgatgactat tactccttct cggaggaagg cctcttctac gagtacgcct acaactggcg
     3001 ggaatacgaa aacccgtgca gcgatgcatt ttacagtccc gataagaaac tggtccgtaa
     3061 tatactggct tcagacctgg gaataattgc caaaagcggt cctgacaaca acctccgcat
     3121 cttcgtcaga gaccttctca ccgctgatcc tgtcgagggt gcaacggtag aggcatatga
     3181 cttccagaac cagctgatgg gttcagccac cactgacagg agcgggatag ccgcaatacc
     3241 ctgtcccagg aaaccctttc tggtgatagc acgaaaggac aagaaccgca actacctctc
     3301 gctgctcgat ggcaatgcgc tgtcaatgag ctcttttgat gtctcgggcg agtcgcccca
     3361 ggatggcata aaggcatttc tgtttactga gcgtgacgtc cgcaggccgg gagactcgat
     3421 ctatgtcggc gtaatcgtgc gcgatcttgg taaagggctg ccggccggcc acccggtaca
     3481 cttcgagctt tacaacagta agggccagcg catcgatgac caggttacca ccctcaacga
     3541 caggggcttc ctcgccttta caaccgtaac ggctgacgat gctgagaccg gcaactaccg
     3601 cgcacaggtg aggataggcg cagcaacttt cacaaaagtt atcaaggtgg agactgtcaa
     3661 gcccaacagg ctgaagatta aacttgactt ccccgatgcc gtggccggcg gagacgacaa
     3721 caccatcaac ggaaacatga aggtcacatg gctcaacggc tcaacggccc gcgacatgaa
     3781 atctaccgtc gagatgctcc tgaaacctgt caaaacctcc ttcgagagat accggcagta
     3841 taacttcgat gatccttccg cacagtttta cttcgagagc cagactatct tcgacggtga
     3901 catcaacagc tccggcgaag cttcggtctc cttctctcct gatgaacaaa tgcaggctcc
     3961 gggcatgctc aacgcgatat tcaccaccag ggtgtttgaa aagggcggtg atgccagcat
     4021 aacccggacc gtggttccgt atgcaccgta ccccgccttc gtcgggatga atattcccgc
     4081 actgggagct gccggacgaa tcatctatac tgaccgtccc aacgaggtca ggctggtgac
     4141 cctcgacaag aacggcaaac cggtccgcag cgaggtggag atcaatgtat acaagctcga
     4201 ttaccgctgg tggtgggaat ctgatgatga atacctcggt tcctatatct ccggcgaaag
     4261 aaatgccaat attttctctg agacaatcac cacctccggc ggtgagggca agacctcatt
     4321 cagcgtcggc aaggaggact gggggcgtta cctggtcagg gcaacactcc cctcggggca
     4381 ctccaccgga atcatagtgc tggttgactg gccgtgggat tacggcatga agccgggagg
     4441 caacgacggc gccagccttc tgcagctcaa cactgacagg gaaaaataca gcgtgggtga
     4501 tgagatcagt ctcaccttcc cggcgccgga gaacggtcgc gccattgtca ccattgagaa
     4561 tgccaccggc ttgcttgacg tgactgaggt caagacccgg gccggcaaca ccgaggtgaa
     4621 gatcaaggca acaccggcca tggctcccaa cgcctatctc tatgttacga tgctgcagcc
     4681 tcactcgcag acggtcaatg atgcgccggt gaggctttac ggggtgatcc cggtgatggt
     4741 tgaggacccg gccacgaagc ttgtgccggt gatcacaatg cctgacaaga tccgctcgca
     4801 gcaggaggtt gagatccgcg ttgaggagaa gaaccgccag gagatgacat acacgctggc
     4861 agtggttgat gatggtctgc ttgacctgac agggttccgt acgcctgacc cgtggaagtg
     4921 gttcttcgtc aagaatgctc ttggagtgaa gacatgggac ctttatgaca tcgtcttcgg
     4981 tgctttcgga ggcaagctgg ggcgcggatt cgcgatagga ggaagtgagg cagccatcga
     5041 ccagagcaag aacagggagc gccgtttcga acctgtcgta aggttcatcg gtcccttcac
     5101 cctcgccccg gggaagacgg cgtcgcataa gatcaggctg ccgcagtaca ccggatcggt
     5161 aagggtgatg gtcaccgcgg ccgggaaagc caacagcttc ggcgcagccg agaaaacagt
     5221 gacggtggct gacccgctga tgatcctggc aacagccccg agggtgctct cgccgggcga
     5281 caaggtagct ctgccggtca ccgtgtttgc acaggataag aagatttccg gtgtcagtgt
     5341 ttccgccacg gcgaacgata tgatcacaat gacggagtca gcaggggcgg taagtttcag
     5401 cgaaccgggc gacaaggatc tggagctgat gttcagcact gcgacgcgga cgggaacggc
     5461 aaaaatcagc gtcagcgccg aggggagcgg cgaaaaggcg tcatacgatc tgacggttcc
     5521 ggtgcggtcg cccaatccgc ccgacaggag ggccgagaca aagtcggtag ccaaaggaca
     5581 aaagttcgaa aagagtttct caccgtttgg catggcggga acatctgcag tgtcggttga
     5641 gatatttacg ctgccgtcag tgaatctgag caagcgcctg aagtatctca cctcgttccc
     5701 gcacggctgt tccgaacaga tcacctccgc ggcgttcccg caggtctacc tgcctgatct
     5761 gctgggatcc gggcttgata atcctgccgt tgtccgcaat aacgtgcagg aggctttgcg
     5821 tgccctctca accaggcaga tggcttcggg ggcagtgaca ctctggcccg gcggcaccta
     5881 tcctgacgac tgggtgacgt catacgtggg acatttcgcc gttgaggcac agaaagccgg
     5941 atatgccatc ccggcagggt tcatccgtaa atggacctca taccagagga agcaggcctc
     6001 ggcatggaga tatcagccac aatatcgtta tacggcaaat gaccaggctt acaggatctt
     6061 tactctggcc ctggccggtt cgcccgacaa gggcgcaatg aaccgcatgc gcgagattac
     6121 cgatctgccg gcactggcca ggtggttcct tgcagctgcc tatgccacaa cgggacggac
     6181 cgaggtggcg cagacgctga ttgacgtgcg gaacctgaag accgaggacg actatgtgaa
     6241 ctattactat ggcagctcgc tcagggacca gtcgataata ctgtataccc tcacgcagat
     6301 gggcaatacg ggcgaatcaa ttgaactgct gaaggatgtg gccgaggcat tgagccatga
     6361 cacgtggtac agcacacaga cgacagcgtg gggcctgttt gcgtacatga actacgcgaa
     6421 gatgatgaag agcgatgcat cgaaaccgct gaaggcgacg ctggagttta acggcaaccg
     6481 tgaaaagctc agtactgacg gcggcgccat catgaagaac ttcactcctg ccgcttccaa
     6541 ccggctggtg gttgagaacg agagcgaaaa tccgctgtat gtgaccgtca ccgaggaggg
     6601 ggtgccttct gtggctgaca tgaccgtgaa ggaaaacaac cttgcaatga aggtcagtta
     6661 tttcaatatg gagtcagaac ctgtcaatgt cagctcgctg gctcagggga ctagtttcat
     6721 aatggctgtt gacgtgacga ataccacgtt ccggcctgtc gttaatctgg ctctgacgca
     6781 gatggtgccg tcggggtggg agatacagaa cacgcgtctc ttcgatgccg ggttgcagtt
     6841 gaaggagggg gcctatgact accgcgacta cagggatgac cgtgtttaca cctatttcag
     6901 tctgcggtca ggtgagacga agaaattctt catcattctg acggcctcgt accgtggcag
     6961 ttacagcatg cccgcggtgg tttgcgaggc actctatgac gagtcttacg ctgcacgccg
     7021 gccgggattt ggtgtagagg tgaaaaaata ggcaagaggc aattggcatt aggcagtagt
     7081 ggaattggtg gcaggccata cagaattgcg atgtgcgatg tgcgatgtgc gattttggcc
     7141 atacagaatt gcgatgtgcg atgtgcgatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnna ttttggccat acagaattgc gatgtgcgat gtgcgatttt ggccatactg
     7321 cattgtagaa ctgtaacatg agatctgttt ttggtcggag gcgtagtccc gatagccgaa
     7381 ggctgtcagg ggcattaaga attgtaatcc ccggcctcgc cgccctcctc ttcgccatcc
     7441 ttgtggtgcc gatgccctca ttccgtgcac ccctctctac cgtggtggag gcagctgacg
     7501 gcaccctcct tggcgcccgt gtggcagcag acggccagtg gcgatttccc ccgcccgaca
     7561 gcctgccgga caaatatatc acctgtctca tcaactacga ggaccgctgg ttccggtggc
     7621 acccgggggt gaatcccgtt gcggttacca aggctctgac cgataatatc agggccggtg
     7681 agacggtccg cggaggcagc accatcacca tgcaggtggc gcggatggcg cgcggcaacc
     7741 cggaacgcac ttatggcggc aagatcattg agatgctctc ggcagttaag ctggagctct
     7801 tccgctcaaa agggactatc cttaagctct atgccgccaa tgcccccttt ggcggaaaca
     7861 ctgtcggcat tgaggcggcc gcctggaggt ataccggtcg cggcaccgcc gacctgacat
     7921 gggctgaagc cgctacgctg gctgtgctgc ccaatgctcc tgccctcatt tatcccggcc
     7981 gtaactccga acggctgaaa gagaagaggg acaggttgct tcagaccctg gctgagaggg
     8041 ggaaaatcga cagcctgaca tgcgagcttg caatggggga gcctttgccg ggagcgccgc
     8101 ttcagatgcc gtcgctggcg ccacacctca ccggaaggct atggattgag gagcccggaa
     8161 caagaaaacg aaccaacgtt gaccctttgc ttcagcgcga ggcatcagaa ctggtcgaca
     8221 ggcatgtttc tgccctggag gggaatggta tccacaacgc cgccgccgtg gtggttgagg
     8281 tgtccaccgg caacgtcgtt gcctgggtgg gtaacagcac cctccctgac accaccggca
     8341 ggcacggccg cgatgttgat atggtcacgg cgccccgcag cacgggcagc atcatgaaac
     8401 cgtttctcta tgcggggctg ctctcctcgg gcatgctcct tcccaacgca ctcatacccg
     8461 atatccccac ccgcttccag ggcttccgcc ccgagaacgc cgacttctcc tacagcggcg
     8521 ccgtgcctgc cggtgacgcg ctggcccgct cgctcaatat accggccgtc cgaatgctcc
     8581 agatgtacga agaagaacgg ttcctgctcc tcctccgcca gatgggcttc accaccttca
     8641 ccaaacccgc ctcccactac ggcctctccc tcatcctcgg cggcggcgag gccaccttgt
     8701 gggaactggc atccgcttac gcatcgatgg caagagtcct gatcaccaac cccggaacgc
     8761 aaacaagcaa ttctagctcg tcaatcccaa atcgcaaatc ccaaatcgca attcctcctc
     8821 tctctccctc ctccatctgg ctgacctatg aggccctccg gcgggtcaac aggcccgaga
     8881 cagaaaccgg gtggcagtat ttcggaagcg cgccggaggt ggcatggaaa accggcacca
     8941 gcttcggctt ccgcgatgcc tgggccatcg gcacaacacc tcagtacatc gttgcggtgt
     9001 gggcgggaaa tgccgacggc gagggtcgtc ccgggctgtc gggcatcgcg tcagcagcac
     9061 ccctcctctt cgacattata aggctccttc caccgtcagc atcctggttc gcgaaaccgg
     9121 atgatgggat gaccctcgtc gaagtatgtg ccgcaagcgg atacagggct ggccccgact
     9181 gcactgacag gaaagatgaa tgggttcctg aggcaggact tcgcagcgaa gcttgcccgt
     9241 atcatacaat tgtcagcctc gatcccacag agacctggag ggtcaacagt tcctgtgccg
     9301 cacccggaga gatggtggcc cgctcctggt tcgtccttcc accggcaatg gaatattact
     9361 atcgcatgcg aaatccgtcg taccggaccc ttcctccctt ccgccaaggg tgttcagatg
     9421 acatgaagct gccctcgatg gagttcatct acccttcgcg cgatgcgcgt attttcatcc
     9481 cccgcagcct ccagggacag ctcatgtcaa tgctgcccga aattgcccac cgcagacgaa
     9541 atgcaactgt ttactggcat cttgacaatc agtacatcgg gatgaccagg catattcacc
     9601 agacagagat cagggtaggt gagggcgagc atctgataac tgccgtagac gaagaaggga
     9661 tgaccgtttc acgcaagttt tactgcatcg gaacattgtg acggcaaatc cggaaaaagg
     9721 attactttta caatcgcata acaagccaac tttaaggaaa agtgaccact ctttcattcg
     9781 ggagtttctt taaacgtatc atcgcgggga ctgccttcaa ccgttacagg caggtttttg
     9841 acagggcggg atatgcgatt atgcttatcg ataacgccac aaaccatttt atcaaggcaa
     9901 acagggcggc atctgagctg ttcggataca ccgaagagga attccggaag atgaccccgc
     9961 tggatctctc aacagagacc ggtgaagaac cgaccctccc cggggagaaa accgaagaat
    10021 ccctgatcag gccggccagg cgcaaggacg gtaaaatcat taccatcaag gtgcagacaa
    10081 gtatcctgga aagattccgg attagcctta tcagcgatgt gaccgaagag taccggatac
    10141 gccgggccct cgagctgaat gaggaacgtc tcagggaagc acagcggata ggccggttcg
    10201 gatggtggga ataccgtccg gagacaaaca ggtatatcgg gtcggaagaa atatcgagga
    10261 tttataacga cgaaccgtca aatctctcgt tcacttacca ggacaatata tcctacacac
    10321 acccggagga cagatcatac ctgataactg ccatagagga tgccctcgaa aaaaagaagg
    10381 agtcttattc tctcacctac aggatcataa cccctaacgg gaaggtgaag gccattgccg
    10441 tcaactcgaa cgtcaggtat gacgaaaatg gccgtctgct ctaccgttac ggcacctgcc
    10501 aggacatcac cgagacccag aagataatgg acgacctcat agcggcgcgg cgccaggctg
    10561 aggagtccga caggctcaag acaacattcc ttgccaattt cagccacgag atacgtaccc
    10621 cgctcaatgc gatgctggga ttcctcaaca ttcttacttc gatggacacc accgaaaagg
    10681 agaaggagga gatggtgaag ctcattgaga tcaactccca gaggcttctg cggcttatca
    10741 atgacatgat tgacatgtcg cgcattgaga gcaacagcat ggagatcaag tacaccaata
    10801 ttgagataaa cggtttcgtc aactcaatgg tctcctcttt caatcatgaa cttgaggcct
    10861 cgggcaagaa ggacctaagg ctggtacctg ttgttgacaa gacgaaggcc gctgtttatc
    10921 agtacattga cgagcttcgt ctgagtcaga tcttcagcaa ccttgtcagc aatgccatca
    10981 aattcactga ccagggcgaa ataaaatacg gttacaggat ttcgcggact gataaccgtt
    11041 actcgatagt ctatttcgtc actgacaccg gtcaggggat aaggcctgac aggcttgatg
    11101 acatcttcct gccgtttgtt cagtactgga atgaggatac cccgaaggta cagggggcag
    11161 gattaggact cgccatctgc aagcggcttg ccgagctgat gaagggacgc atctgggtgg
    11221 agtcgctgcc cggcaaggga tcgtcgttcc tcttctcgtt caacacgccc gtgatgatcg
    11281 agtaattgca aaattcaaat tttgtttatc tttgtccccg cccgggacgt tagctcagtt
    11341 ggtttagtcc gccaac
//
LOCUS       DAOPXU010000087        11329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_6548_length_11329_cov_13.152652, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000087 DAOPXU010000000
VERSION     DAOPXU010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11329
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6548_length_11329_cov_13.152652"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="PKK03_09025"
     CDS             <1..1227
                     /locus_tag="PKK03_09025"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNX84583.1"
                     /translation="RGSEFTGWLRLPDSVVNETAAVKACAARLRSQAPVTVVVGIGGS
                     YLGARALTEALNDPFAPKEHTLLFAGHTLSEDYHAALLRFLDGTAYNIVVISKSGTTT
                     EPAIAFRVLRDHLRNKMGVAAMKSHIVAVTDARRGALHDLAVTEGYETFVIPDDVGGR
                     YSVLTPVGLLPLALAGYDIDAFARGMLDMALSVREGNDTPANIAVTYAAARNTLYSIG
                     YSTEILISYEPSLVFLAEWWKQLYGESEGKESKGIFPASVTFTTDLHSMGQYIQQGER
                     KLFETVIHVADSRNKLVVPGATDNSDGIGFIAGKRMTEVNHKAEDGTRIAHTDGGVPV
                     IMVSMERIDEYNLGQLMYFFELACAISGYVLGVNPFDQPGVESYKKNMFALLGKPGFE
                     AERERLEQQLKTQAGL"
     gene            complement(1212..1832)
                     /locus_tag="PKK03_09030"
     CDS             complement(1212..1832)
                     /locus_tag="PKK03_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84584.1"
                     /translation="MNKLRHMELSELLKPLNSPVKIQEFLDSIPYNSTKRTLSPLLVM
                     KERMAHCMDGGMFAAAALRRLGYLPLIVDLTAENDDDHIIAVFREAGCWGAVAKSNTT
                     VLRFREPVYRTLRELAMSYFDLYYNLNGQKSLRSYSRAIDLSRFDDRDWETTGEDLEY
                     IGDHTYTMRHYPLITPAQAKRLNHVPKYLYDAGFAGADIDGLYKPA"
     gene            complement(2095..3600)
                     /gene="lysS"
                     /locus_tag="PKK03_09035"
                     /pseudo
     CDS             complement(2095..3600)
                     /gene="lysS"
                     /locus_tag="PKK03_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
     gene            complement(3729..4334)
                     /locus_tag="PKK03_09040"
     CDS             complement(3729..4334)
                     /locus_tag="PKK03_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84585.1"
                     /translation="MKKLIITMILLAGGGIIWSQDNGSLNFSGSYIYMPQYRMSGAGY
                     TISLQHSLKPWLRLEIGGSYTLGSRTIRRNETVNGVKLLDLNYHHAAYSLYAAPVVTA
                     GNGGAVSLDVYAGPVITWQSNVFDLNRYEMPESPDYIGRMTNIVYLNRVLEGTFFGGI
                     AGCRVNVRTGENWRVNFGISTTGIIKAVSSVNASIGIRYCR"
     gene            complement(4345..5220)
                     /locus_tag="PKK03_09045"
     CDS             complement(4345..5220)
                     /locus_tag="PKK03_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84586.1"
                     /translation="MKTPIILSILLSAVILASCDPEELTSPVALFSAQTSPIYMFKVN
                     LDASGSFSTEEGDWLEYRWDLNGDHLGWETDWSNSPVVTVQFPYDNSGYIGLQVRNSD
                     GNITELYQGFYTDVNYGIIRPFTDLQIGFRTINYQFSYPDHHWSWMWSYDNIQLPDSD
                     SWYNYTASADRAAYGTLMPWTVADTLEKDYDLPSKADWQKMIDFCGGAVLAGFNLQVE
                     TEHGLRLTLPGIVTEGQLQEKNTAGYYWTGDEADGDSAWALKISAESDKAEFVILEKS
                     SLASVRLMIEFLNYR"
     gene            complement(5231..5917)
                     /locus_tag="PKK03_09050"
     CDS             complement(5231..5917)
                     /locus_tag="PKK03_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84587.1"
                     /translation="MKKSALIFLICAASLTGTVCLAQTDTVKIETELQQDQEYYYRNK
                     YRYLDVRLGDEDRLLKIGIQPFKPSDNFNFLVFTLHGGFEQKINTSLSAVTEINSIMT
                     WSEGEAFHLQGLSLGGRYYFLKKSDIEKGRSGNNLQGVYGLFKMSDLLSLVTTRSESS
                     GYDPSSHNPHLERFIKGSLTPEIGIGFQQRLDYHLYFDSALSCNYSILDDDFGFQIRV
                     LFGVLFSYTK"
     gene            complement(5924..6547)
                     /locus_tag="PKK03_09055"
     CDS             complement(5924..6547)
                     /locus_tag="PKK03_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84588.1"
                     /translation="MFCLAALALVLLMSREANAQDFRESPYNKRIFSFNSGAVFSGDG
                     DCWGIGTGVSHLKTIGRRFYIRENLTSWIINGESWIEGAFENQTAIDLSAELGFSPFR
                     IGQRFFSVHGGISGAYFINTDATGGGTWLTYNPATGDYSYMQYFEQKLARTPDFGITF
                     GVNYHRQVSPSLYLNARADFRSHLSTGSAISMITIGVGFDARELFKK"
     gene            complement(6606..7463)
                     /locus_tag="PKK03_09060"
     CDS             complement(6606..7463)
                     /locus_tag="PKK03_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84589.1"
                     /translation="MKILQHMVMCLVLLGTCDLAAQEVMQKASRTDERSFGKGISGVS
                     VYGEKATVTVRGTAVSELKVLLRPVSRNRNKELAVADLKYIHYSAEKEGDILVIRNSF
                     RGQPEQITSNLSMEIEIIMPSSLPVSITNLYGPVEAGNLASMTANVSFGSLRLTDIGS
                     ATTVTARYSDMELTSIRGKADIKAEKSDILMTALGAATVINCSYGTANLELAGSGPLI
                     VKGYRTTVNISLDEPGKFQYALKAPQGRIIMSDGKQYRNQPVEVIPQVPAGLIDVTTS
                     YCDITIAKK"
     gene            complement(7460..8209)
                     /locus_tag="PKK03_09065"
     CDS             complement(7460..8209)
                     /locus_tag="PKK03_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84590.1"
                     /translation="MNKEFDDILSERIRELPSRLPSANLWREIEAALDMEDTISSQLR
                     DLPLHQPSAAIWETIEAALQDGHQDGYSGADVRGPVKRTLQIPSERADTLLPGEKRPG
                     LRRRVLLLAGEKRAGLSRRVLLLTTAGVAAVVLILFMIPRLVRTEKGITVESEIVLSE
                     EHGTPVTPAGEDEDPIEVIKDLCKTGAPVCQSELFREKMQLYQELNEELRQLETVIGQ
                     VGDSPEIIQSVIRIENLKSGTLQELIQLIHS"
     gene            complement(8193..8741)
                     /locus_tag="PKK03_09070"
     CDS             complement(8193..8741)
                     /locus_tag="PKK03_09070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNX84591.1"
                     /translation="MQQSELNQNDRELVRGCLENNRLVQRALYERFSTRLYTLVYRLI
                     SDRDASNDVLQDSFIEIFRSMATFRFESSLFTWMRTIVIRNTMNYLRKNARLQVATDE
                     IPDQASEGSFNFTAEHLEKAILSLPAQARSVFILIEVEGYKHQEVAEILNINEGTSKS
                     QLNYAKKLLRNRLTKPSDEQRV"
     gene            complement(8988..9845)
                     /locus_tag="PKK03_09075"
     CDS             complement(8988..9845)
                     /locus_tag="PKK03_09075"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNX84592.1"
                     /translation="MEVVYLNGQFLPKDQALISPDDRGFIFGDSVYEVARWYGGYFFD
                     LTGHTGRFRRSMKETHMVWEDEAKLEEIFEELIQRNELGAECAIVYFQATRGVAPRNH
                     AFPDPPVKPTIYGFARRQSINTLASERGVAIFLTPDPRWNRCDIKTTALIANILPYQE
                     AHDQGFAEVCFVRNGFVTEGAHSNIFFVRDETLYTHPGNNDILSGITRKNIISVARTN
                     DISVVEEAVAADMIPYMNEAFISNTTGEIVPVTRISEQVIGEGTPGHLTRKLQVLLRE
                     FIQSCDPRR"
     gene            10015..10884
                     /gene="lipA"
                     /locus_tag="PKK03_09080"
     CDS             10015..10884
                     /gene="lipA"
                     /locus_tag="PKK03_09080"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNX84593.1"
                     /translation="MRQSEFMTGKRRLPSWLRMERASGESYTMVKNLVESHRLHTICT
                     SGNCPNIGECWNAGTATLMILGDICTRSCKFCGTKSGRPLPPDPGEPERVAGAVKTMH
                     LKHCVITSVDRDDLPDGGAAHWAETIRKIKEVNPGVTIETLIPDFMGETEHIDMVIDA
                     GPDVISHNIETVRRLTPLIRSVAKYEVSLAVLRHIASRGRRAKSGIMLGLGETEEEVT
                     EALTDLYATGCKIVTIGQYLAPSLEHMPVVEYVTPEKFEEYRKRGLETGFEFVESSPL
                     VRSSFHAEKHVGK"
     gene            10937..11311
                     /locus_tag="PKK03_09085"
     CDS             10937..11311
                     /locus_tag="PKK03_09085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNX84594.1"
                     /translation="MKSYKDLDIYNEAFRLALAVHVMTQKLAKAEHYELGSQVRRAAQ
                     SVRSNIVEGYGRREYKNEFIRYLIFARASLLETGSHLEMSRSLYENIEIAGLIEDYNL
                     LGKKLAAFIHYVESSWMTPHDK"
BASE COUNT         2666 a         2861 c         2894 g         2908 t
ORIGIN      
        1 cggggaagtg agtttaccgg ctggctccgt ttgcctgaca gtgttgtcaa tgagaccgcg
       61 gctgttaagg cgtgtgcagc aaggctgcgt tcccaggcgc ctgtgacagt ggttgtcggc
      121 ataggtggct cataccttgg cgccagggct ctcaccgagg cactgaatga ccccttcgct
      181 ccgaaggagc acacgctcct ctttgcaggc cataccctca gcgaggatta ccatgcggcc
      241 ctgctcagat tccttgacgg cactgcctat aatatagtcg taatctcaaa gagcggcacc
      301 accaccgagc cggcaatagc cttccgggtc ctccgtgacc acctcaggaa caagatgggg
      361 gtggctgcca tgaagtcaca tattgttgct gtcactgacg cccggcgagg tgcattgcat
      421 gaccttgccg tcaccgaggg ttatgagact ttcgttatcc ctgatgatgt tggcggccgc
      481 tattcggttc tgacaccggt tggattactg cctcttgccc tcgcagggta tgacattgac
      541 gcttttgccc gtggaatgct tgacatggcc ctctccgtaa gggaggggaa tgatacgcct
      601 gcaaacattg ccgtaaccta tgcggcagca cgtaacaccc tgtattcaat tgggtattcg
      661 acagagatcc ttataagcta cgaaccatcg ctggttttcc ttgccgagtg gtggaaacag
      721 ctctacggcg agagtgaggg caaggagagc aagggcatct tcccggcatc ggtgacattt
      781 acaacagacc tccattccat gggacagtat atccagcagg gcgaacgcaa gctgttcgag
      841 acggtgatcc acgtggcgga ctcgcgtaat aagcttgttg tgcccggcgc cactgacaac
      901 tctgacggca tcggcttcat cgccggcaaa aggatgaccg aggtaaacca caaggctgag
      961 gacggcactc gcattgcgca tacagacgga ggtgtacctg tgataatggt cagcatggag
     1021 cgtattgatg aatataacct gggccagctg atgtatttct ttgaactggc ctgcgccatc
     1081 agtggttatg ttttgggcgt caatcccttt gaccagccgg gggtggagtc atacaagaag
     1141 aatatgttcg ccctcctggg caagcctggt ttcgaagccg aacgcgaacg gctcgagcaa
     1201 cagttgaaga ctcaggctgg cttatagaga ccatcaatat cggcaccggc aaagcctgca
     1261 tcatagagat atttcgggac atgattgagc ctcttcgcct gggccggtgt tatgagcggg
     1321 tagtgtctca tcgtgtacgt atgatcgcca atgtattcaa ggtcttctcc tgttgtttcc
     1381 cagtcgcggt catcaaatcg cgaaaggtca atagcacgtg aatagcttcg cagcgatttc
     1441 tgcccgttca ggttataata gagatcgaaa taggacattg ccagttcgcg aagtgtccgg
     1501 taaaccggtt cgcggaagcg caatacggtg gtatttgatt tcgcgacggc gccccagcac
     1561 cctgcctcac ggaatacggc aattatatga tcatcatcat tttctgcagt cagatcgacg
     1621 ataagcggta ggtatccaag cctgcggagg gcagcggcag caaacatccc gccatccatg
     1681 cagtgggcca tccgttcctt cattaccagc agtggcgaga gtgtccgttt tgttgagttg
     1741 tacggaattg aatccagaaa ttcctggatt ttcaccgggg aattgagagg tttcagcagc
     1801 tcactcagct ccatgtgtct cagtttattc acgaatgtcg gatatacaat gctgtatggc
     1861 ctacggtcaa atttatttta tgttgctctt ctacaatgct ttatggccta cggctaatct
     1921 gtataatatg ctactggtct acaatgctgc atgctctgcg gataaaatct cacattgcaa
     1981 atcgcacgtc tcaactccaa gtatttttta ttatcagcca tcaacagtcc tgcaaagctg
     2041 tattacaggc caaattaatt cgcaactcgc aattcgcaac tcgcaaatct aagcctccgg
     2101 cttcatctgc gggaagaaca gcacatcctg tatcgagggc tggttggtca tgaacatggt
     2161 gagcctgtcg atacccattc ccattcccga ggtaggaggc atggcatatt ccaaagccct
     2221 gacgaagtcc atgtcgataa acattgcctc gtcatcaccc ttttccgaga gacgaagctg
     2281 atcctggaac ctctcgagct ggtcgattgg gtcattcagc tcggagtagg cgttggccag
     2341 ctccttgccg ttgaccatca gctcgaaacg ctcggtcata ccatgcttgc tgcggtgttt
     2401 ctttgtcagc ggtgaggtct cctgcgggta gtcaatgatg aatgtgggct ggatgaagtg
     2461 atgttcgcac ttctctccga aaatctggtc aatcagcttg ccctttccca tcgagggaga
     2521 gtggcctatc tccatcctgt cgcactcccg gcgcaggtca tcctcagtca tgtctgttat
     2581 gtcaatgccg gcatattctt ttatggcgtc ggtcatggag atacgcctgt agggagcctt
     2641 gtagtctatg acatgctcac cgagaggcac ctttgtggtg ccgtggaggt ccatggccac
     2701 cttctcaagt atcttttctg tgaactccat catccagttg tagtccttat aggccacata
     2761 gatctccatc actgtgaact cgggattatg cgtgcggtcc atcccctcgt tgcggaagtc
     2821 ctttgcaaac tcgtagactc cgtcataacc gcctactatg agacgcttca ggtaaagctc
     2881 gtccgctatc cggaggtaga gaggtatgtc gagggcatta tgatgagtta tgaatggtct
     2941 tgctgcggca ccgccgggaa taggctgaag cacgggtgtc tccacctcga ggtagccata
     3001 agagtcaaag agctcacgca tcgacctgat tatctgcgtg cgcttgcgga aaacctcacg
     3061 gacttcggga ttgatgatca ggtcgacata acgctggcgg tatcgcatct caggatcagt
     3121 caccgcatcg tacagaaccc cgtccttttc ctttactatg ggaagagggc ggagcgactt
     3181 gctgaggagg gtcagctcct tcacatgaac ggtgatttcg cccatctggg tgcggaatgc
     3241 aaaacccttc actccgatga tgtcaccaat atccagaagc tttttaaaca gggtattgta
     3301 caatgttttg tcctcgccct ggcagaggtc atcacgacgt acatagattt gtatgcggcc
     3361 agaagagtcc atcagctcgg cgaatgaggc gttgcccatg atacggcggc tcatgatccg
     3421 gcctgccagg catacatccg tgaatttatt ttcagtgtca ctgaatcctg ccagtatttc
     3481 tgcggccgaa atgttaaccg ggtacattgc agcaggataa gggtctatgc caagtttgcg
     3541 tatttcttca agtgattgcc tgcgaaccag ttcctgctcg ctcagttggg gtgtgctcat
     3601 gttacgaaaa atttttgcaa aggtagcact tgagaggcaa tagacaatag cagaaattca
     3661 ccgaaggcga ttcatccggt cacaattctt attgccctgc gggcgaaggt gctgcggggt
     3721 tctgccagtt accggcagta acgaatcccg atgctggcat tgacggatga aactgccttg
     3781 attatgcccg tggtactgat accgaagttc acccgccagt tctccccggt acgtacatta
     3841 accctgcagc ctgctatacc tccgaagaag gttccttcga gcaccctgtt gagatacaca
     3901 atatttgtca tccttccgat gtaatcaggg ctttcaggca tctcataacg gttcaggtca
     3961 aacacatttg actgccaggt gatgaccggg cctgcataaa catccagtga aaccgccccg
     4021 ccgttgccgg ctgtcacaac cggtgccgca tacagactgt aagccgcatg atggtaattg
     4081 agatccagca gcttcacgcc attgacggtt tcgtttctcc tgattgtccg tgacccgaga
     4141 gtatagcttc ccccgatctc gagtctgagc cagggcttca gactgtgctg cagggatata
     4201 gtgtaacccg caccgctcat ccggtactgt ggcatataaa tatatgagcc cgaaaaattg
     4261 agggatccat tgtcctgtga ccagattata ccgccgccgg caagcagaat cattgttata
     4321 atcagctttt tcatggtttc cgggttatct gtagtttaag aattcaatca tcagtcgcac
     4381 cgaggccagg cttgattttt caagtatgac gaactctgcc ttatcgcttt cagccgagat
     4441 cttcagtgcc caggctgagt ctccgtcggc ctcatcacca gtccagtaat atcctgcggt
     4501 attcttttcc tgcaactggc cttcagtaac tattcccggc aaagtgagcc gcagtccgtg
     4561 ctctgtctcc acctgcagat tgaaacctgc caggacagcc ccgccgcaga agtcaatcat
     4621 cttctgccag tcggcttttg agggaaggtc ataatctttt tcaagggtgt ctgccacagt
     4681 ccagggcatc agggtaccgt atgcggcacg gtccgcagaa gcagtgtagt tgtaccaact
     4741 gtcagagtcg ggaagctgaa tgttgtcata agaccacatc catgaccagt ggtgatcagg
     4801 ataagagaac tggtagttta ttgtacggaa tccaatctga agatctgtaa aaggcctgat
     4861 aattccgtag ttcacatcgg tatagaagcc ctgataaagc tctgtaatat tcccatcgct
     4921 gtttctaacc tgcaaaccga tataaccgct gttatcgtaa gggaactgca cggtaacgac
     4981 aggactgttg ctccagtctg tttcccaccc gaggtgatca ccgttgagat cccaccggta
     5041 ttccagccag tcaccctcct ctgttgaaaa ggaaccggac gcatcaagat ttaccttgaa
     5101 catataaatg ggagacgtct gagcgctgaa aagggccaca ggcgatgtca gttcttcagg
     5161 gtcacaggat gcaaggataa ccgccgatag taaaattgat agtattatgg gtgttttcat
     5221 ggcaggtagt ctatttggta taactgaaaa gaacgccgaa cagaaccctg atctggaatc
     5281 cgaaatcgtc gtcaaggata ctgtagttgc atgagagagc cgaatcgaaa tagagatggt
     5341 agtcaagcct ctgctggaag cctatgccta tttcgggagt cagtgaacct tttatgaatc
     5401 tttccagatg aggattatga gatgacggat cataaccgct gctttctgaa cgggttgtta
     5461 caagtgagag aaggtcgctc atcttaaaga gtccgtaaac tccctgcagg ttgttgccgc
     5521 tgcgcccctt ttcaatgtca ctcttcttga ggaaatagta acgtcctccg agtgacagcc
     5581 cctggagatg gaatgcttcg ccctcagacc atgtcattat cgagttaatc tctgtaactg
     5641 ccgaaaggga agtgttgatc ttctgttcaa atccgccatg cagtgtaaag accaggaaat
     5701 tgaagttgtc actgggcttg aaaggctgga taccgatctt gagcagcctg tcttcgtctc
     5761 ccagccgcac atcgaggtag cggtacttgt tccggtagta atactcctgg tcctgctgaa
     5821 gctctgtctc aatcttcacc gtatcggtct gtgcaaggca gactgtgccc gtaagtgagg
     5881 ccgcgcaaat tagaaatatc aatgctgact ttttcatttc ctgttacttt ttaaatagtt
     5941 cgcgggcatc aaagccgaca ccaatagtga tcatcgagat tgctgatccg gtacttaaat
     6001 gtgaccggaa atcagcccgt gcgttaaggt acagggaagg agagacctgc cggtggtagt
     6061 tgactccgaa agtaattccg aaatcaggcg ttctggcaag tttctgctca aaatactgca
     6121 tgtagctgta atctccggtg gcaggattgt aggtaagcca tgtcccgcct ccggtcgcat
     6181 cagtgtttat aaaatacgca ccgctgatgc caccatgtac gctgaagaaa cgctggccaa
     6241 tcctgaaggg agagaaaccc agttcggcac tcaggtcaat ggcagtctga ttctcgaaag
     6301 ccccttcaat ccagctctct ccgttaatta tccatgaggt caggttctcc cgtatataga
     6361 acctcctgcc tatagtcttc aggtgagaaa cgccggtgcc gattccccag caatcgccgt
     6421 cgcctgaaaa gacagccccg ctgttaaagc tgaagatccg cttattgtaa gggctctccc
     6481 tgaaatcctg tgcatttgct tcccggctca tcagcagaac cagggccagg gctgccagac
     6541 aaaacatatg atgccttttc tgcaagaccc ctgaaatcgg tttatccttc gttttcatag
     6601 acggattatt tctttgctat tgtaatgtca cagtatgaag tggttacatc aataagtcct
     6661 gcaggaacct gtggtatgac ctccaccggc tggttccggt attgtttccc gtcggacatg
     6721 attatccttc cctgcggtgc cttgagtgcg tactggaatt tgcccggctc gtcaagggag
     6781 atgttaaccg tggtacggta tcccttgaca atcagtggtc ctgatccggc cagctcaagg
     6841 tttgcagtgc cgtacgagca gtttatgact gttgcagcac caagggccgt catcagaata
     6901 tcagactttt cagctttaat atcagccttt ccacggatgg atgtcagttc catgtccgag
     6961 taccgggcag ttacagtggt cgctgagcca atgtcagtga gccgcagcga gccgaaggaa
     7021 acattggcag tcattgaagc gaggttaccg gcttccaccg ggccatacag attggtgata
     7081 ctcacgggca gggatgaagg catgatgatc tcgatctcca tgctaaggtt gctggtaatc
     7141 tgttccggtt gtcccctgaa tgaattcctg atcaccagga tgtcaccctc tttctcagct
     7201 gaataatgaa tatatttcag atcagctacc gccagctcct tattcctgtt ccgggagaca
     7261 ggcctgagca gcaccttcaa ctcgctgacg gctgtgcctc tgacagttac ggtggccttc
     7321 tccccatata ccgagactcc gctgatcccc tttccgaagc tcctttcatc agtccttgat
     7381 gccttttgca ttacctcctg agctgcgaga tcgcaggttc cgagcaggac gaggcacatt
     7441 accatgtgtt gcaggatttt catgaatgga tcagttgtat gagttcctgc agggtgcccg
     7501 acttaagatt ttcgatccgt atgaccgact gtatgatttc gggagagtcg cccacctggc
     7561 cgatcactgt ttcgagctga cggagctcct cgttcagttc ctggtagagc tgcatttttt
     7621 ctctgaatag ctctgactgg cataccggtg cgccggtctt gcagaggtct ttgatgactt
     7681 cgatcgggtc ttcatcttcg ccggccggag tcacaggagt gccatgctct tcactcagga
     7741 caatttcact ctcaactgta atgccttttt ctgtgcgcac aaggcgaggg atcataaata
     7801 aaatcaggac aacggccgca actccggctg ttgtcagaag gagtaccctt cggctaagcc
     7861 cggcacgctt ttccccggcc agaaggagta cccttcggcg aagtccgggt cgcttttccc
     7921 cgggcaaaag ggtatcagcc ctttcagagg gtatctgcag agtccttttc accggcccgc
     7981 gcacatctgc gcctgaatat ccgtcctgat gcccgtcctg caacgcagcc tcaatggttt
     8041 cccatatagc agctgacggc tggtgcaagg gcagatcccg gagctggctg gagatggtat
     8101 cttccatatc aagggcagct tctatctccc tccagaggtt cgcggaaggt aagcgggagg
     8161 gcagctccct tattctttcg gataatatgt catcaaactc tttgttcatc tgacggtttt
     8221 gtcagtcgat tccttaacag cttctttgca taattcaact gggactttga ggtcccttca
     8281 tttatattca gtatttcggc cacttcctgg tgcttatatc cttctacctc aatcaggata
     8341 aacacgctcc gtgcctgtgc cggcagtgag agtatcgcct tctcgaggtg ttcagcggtg
     8401 aaattgaacg agccctctga cgcctggtcg gggatctcgt cggttgccac ctgcagccgg
     8461 gcatttttcc tcaggtagtt catcgtgttt cttattacta tggtgcgcat ccatgtaaag
     8521 agcgacgatt cgaacctgaa ggttgccatt gaccggaata tctcaatgaa cgaatcctga
     8581 agaacatcat tactggcatc gcgatcgctt ataagcctgt ataccagggt atagaggcgg
     8641 gtgctaaacc tttcgtacag ggcgcgctgc accagcctgt tgttttccag gcatccccgg
     8701 acaagctccc tgtcgttctg gttcagttca ctctgctgca tcttcgtctt ctgtctatat
     8761 agtgcggatc aggccggaaa aggttggaag agagagaaaa taatttatgt gtccagctaa
     8821 tatcatggat tcagacagcc tgacattcaa ggggcaagct attcatacaa aattaccggg
     8881 attgtttgta atatacaatt gtttatccgc tacgcggaaa ggaatggtgc ccgcggctgg
     8941 tctacaatgc tttatccgct acgcggaaag aggggggatg ccgctgtcta ccgccgcgga
     9001 tcacaggatt gaatgaactc cctcagcaga acctgcagct tccgtgtcag atggcccggg
     9061 gtaccctccc cgatgacctg ttcactgatc cgcgtcacag gcactatctc tccggtagtg
     9121 ttggagataa acgcttcgtt catgtacggt atcatgtcag cggcgacagc ctcctcaacc
     9181 acgctgatat catttgtcct cgcaacggag ataatgtttt tccgggtgat cccggagagg
     9241 atatcattat tccccggatg ggtgtaaagt gtttcgtccc tcacaaaaaa gatatttgaa
     9301 tgagctcctt cggttacaaa gccattccgt acgaagcaca cctctgcaaa tccctggtca
     9361 tgtgcctcct gataaggcag gatattggct atcagtgcag tggtcttgat atcgcagcgg
     9421 ttccaccgtg gatccggggt gagaaagata gcgacacccc gctcggaagc aagtgtattg
     9481 atgctctggc ggcgggcaaa tccgtagatg gtcggtttga ccggaggatc gggaaaggcg
     9541 tggttgcgcg gcgcaacacc ccgggtggcc tggaaataaa ctattgcgca ttcggcgcca
     9601 agctcatttc gctgtataag ctcttcaaag atttcctcga gctttgcttc atcttcccat
     9661 accatgtgtg tctccttcat gctgcgcctg aacctgccgg tatggcccgt gagatcaaag
     9721 aagtacccgc cgtaccatct tgcaacctcg taaacactgt ccccgaaaat aaaaccccgg
     9781 tcatcagggc tgatcaatgc ctgatccttc gggagaaact gaccgttaag atatactact
     9841 tccatatttt aagatttaca ctttgccggt tgggtcatga tggcctgcga ctctgatttt
     9901 ttgcccatac gggaatctga aaattaatct tcaaagatac attttcaatg gattggtcat
     9961 acaaatttca acccatcctt tctcttttat tatgtacctt tgcatattca cgaaattcgg
    10021 caatcagaat ttatgacagg caagaggcga ctgccatcat ggctcaggat ggagagggca
    10081 agcggggaga gctacaccat ggtgaagaac ctcgtggaga gtcatcggct gcataccatc
    10141 tgcacctcgg gcaactgccc gaacataggg gagtgctgga acgccggcac tgccaccctg
    10201 atgatcctcg gtgacatctg tacaaggtca tgcaagttct gcggcacgaa gagcggccgt
    10261 ccgcttcccc ctgatccggg ggaacctgag agggtggccg gtgcagtgaa gacgatgcac
    10321 cttaaacatt gtgtcatcac ctcggttgac cgtgacgacc tgcctgacgg cggagctgct
    10381 cattgggcag aaacgatacg taaaatcaag gaggtgaatc cgggagtgac catcgaaaca
    10441 ctgattcccg acttcatggg tgagacagaa catattgaca tggttattga tgcagggccc
    10501 gatgtcatct ctcataacat agagacagtc aggcgcctga ctcccctgat caggtcagtc
    10561 gcaaagtacg aggtaagcct ggcggtactc aggcacatcg cttcgcgcgg taggcgcgca
    10621 aaatccggaa ttatgcttgg cctgggtgag accgaagagg aggtgaccga agcgctgacc
    10681 gatctttatg caaccggttg caaaattgtg actatcgggc agtatcttgc tccaagcctt
    10741 gagcatatgc cagttgttga atatgttacg cccgagaagt ttgaagagta caggaaaagg
    10801 ggactggaga ccggatttga attcgttgag agcagtccgc ttgtcagaag ttccttccat
    10861 gctgaaaagc atgtgggtaa atagtggtta tcggttatcg gttatgggtt atcggttatg
    10921 gttatcggtt aggtgaatga aaagttataa ggatcttgac atatataatg aggctttcag
    10981 gttggcgctg gctgttcatg ttatgacaca gaaactggct aaagcagagc attatgagct
    11041 tggaagccag gtgcgaagag ctgcacagag tgtgagatcc aatatagtgg aagggtatgg
    11101 caggagagag tataaaaatg agttcataag atacctgatt tttgccaggg cctcattgct
    11161 tgaaaccggg tctcatcttg agatgagcag aagtttgtac gaaaatattg aaattgccgg
    11221 attgatcgaa gattataatc tgctgggtaa aaagctcgct gcattcattc actatgtgga
    11281 atcctcctgg atgacccccc atgataaata acagacaata accgataac
//
LOCUS       DAOPXU010000088        11151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_6707_length_11151_cov_14.114185, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000088 DAOPXU010000000
VERSION     DAOPXU010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11151
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6707_length_11151_cov_14.114185"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2336)
                     /locus_tag="PKK03_09090"
     CDS             complement(<1..2336)
                     /locus_tag="PKK03_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNX84595.1"
                     /translation="MRDKKFRKNIIWFWVIISLPAVFLLTLFLLISANRLGPIPSFEE
                     LENPDNSLAAEVYSEDNVLLGKFYIQNRTWTDYEDISPYVIDALIATEDIRFHRHSGI
                     DFRGLARVLVKTIMLGQNTGGGSTITQQLAKNLFLPRDLSNDPAIVRASKLAVSKFKE
                     WQTAVKLERSYTKEEIITMYLNVFDFIYNAVGINSAARIYFNTTPDSLNIEQSAMLVG
                     MLKNSVAYNPLRNEESALKRRNVVISQMERYGYISRALADSVSQLPLGLDLREDSHNT
                     GPATYFREYIRSTMNSKEPQRDRFFSDDAYNEALWRWQNDPLYGWCYKNTKPDGSPYN
                     IYRDGLRIYTTLNSKMQTYAEEALAEHLSKNLQPVFDKRARNYRNPPYSNDLSTAQVN
                     QIMARSVRQSDRYQSMRRAGVPEDSITLAFNTPVPMKLFSWQGERDTVMTPLDSIRYY
                     KFFVRSSFMAMDPHTGHVKAYIGGPDFRYFKYDAVTQQKKQVGSTIKPFLYTLAMQDG
                     YSPCYEVENIERTFEVNDTIWRPRSSGPEEYHGKMVTLKWGLAQSENYISAWLMKQFS
                     PQSVVDIMHRMGIRSYIDPVVSIFLGTSDLSVEEMVGAYGTFVNKGVYTRPLYVTRIE
                     DRNGNVISTFTPFIDEVISEDDAYLMTSLLQGVVSSGTAVRLRLTYKLTNQMGGKTGT
                     TQNHSNGWFMGVMPDLVAGVWTGWEDQAIHFEDLSMGQGANMALPVFGIFMQKLLADK
                     DFSVIAESQFEAPPGFNVDLDCDRVNREQQGNPYRQRE"
     gene            2448..4766
                     /gene="topA"
                     /locus_tag="PKK03_09095"
     CDS             2448..4766
                     /gene="topA"
                     /locus_tag="PKK03_09095"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNX84596.1"
                     /translation="MTDNLVIVESPAKAKTIEKFLGKDYTVVSSFGHIRDLVRNNLGV
                     EVDNNFAPVYEIPEDKKKVVAELRKLADKATTVWIASDEDREGEAIAWHLISVLGLDP
                     ATTRRIVFHEITKDAITRAVESPRQVDMNLVNAQQARRILDRLVGFELSPVLWKKVQP
                     SLSAGRVQSVAVRLLVDREREIIGFVADSAFRVTGTFSGTNSEGNEVQLKAECPKKFS
                     TEEDARLFLEKCDASAFTVENIVVKPGTRSPAPPFTTSTLQQEAYRKLGFSVAQTMSV
                     AQKLYEAGKITYMRTDSTNLSSLALNTAREVITNEYGAAYSRTRQFKTHSRGAQEAHE
                     AIRPTYFDRKEVTGNNNEKKLYELIWRRAIASQMADARVEKTTITIGMSASPVSFTAT
                     GEVILFDGFLKVYTESYDSNASEDEKAILPRVDQGMKLTPGTITALQRFTSPPPRYTE
                     ASLVKKLEELGIGRPSTYAPIISTIQSRGYVAREDRPGEKRTLAQLALSKGKITRSEK
                     TEIAGKEKSKLFPKDIGMIVNDFLIANFPDILDYNFTAGVEKEFDEIASGEVKWEKMI
                     STFYGPFRKTVDESLATRAKMTGARQLGTDPATGLPVAVKMSRFGPVVQLGEASEEQK
                     PRFANLRRDQLLETITLEEALALFALPRAVGTFEDSEMTVGVGKFGPYVKHAGKFYSL
                     KRGVDDPYTITGDRAIELITEKREGDKQKVISELGDIMVLNGRYGPYIMHDKTNYRIP
                     KGTDPHKLTRDECLAIIEKGSKTKTTRGRKKK"
     gene            4778..5947
                     /locus_tag="PKK03_09100"
     CDS             4778..5947
                     /locus_tag="PKK03_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HNX84597.1"
                     /translation="MIDLNQYIDPVCLDKPGVEPLKGSSAFPHNMLVNTGNEPLGDLS
                     GFAVAILGVPDDRSSVNTGAAQAPDAVRRSLYSFSRLPGRLKVTDLGNFRPGASFDDT
                     IAGLRDVLLQLLSQNIVPVIIGGTSSLMPAVDRALSAGKQKWSMVSVDSRIDFTAEKR
                     PPDSLNWMNDLIYRSGSCLSHLSAIGHQTYLTDQQVVNRFNRRHYDMMRIGEVRSAVH
                     ETEPLFRDASAALFDIGSVRQSDAPGTFLPSANGFYGEEICLLARYAGLSDNLKIFSV
                     TEVNPALDTRMQTSHLAAQLVWFFLEGFSQKQYEVSYLSDQTNSRFTRYHVTLSDLES
                     EAIFIKSTITERWWMEIRNTRGEPQYLACSYEDYLMANRDQVPVRWSRGMLRHGH"
     gene            6031..6975
                     /locus_tag="PKK03_09105"
     CDS             6031..6975
                     /locus_tag="PKK03_09105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNX84598.1"
                     /translation="MKKTRILILALLLPAGIVCGQQDPVFSHYMFNTQTYNPGFSGMS
                     GMITASALTRQQWVGFPGAPSTMLFNINMPFSLFGFRSGAGLLVEADKYGFSNDIDIT
                     LSYSGLFSVGTGTLGAGFSIGVLNKTVSPEWFIPSGPGHTPPTGDPLIPENDESFLAA
                     DFSAGVYYQGLNYYAGLSVTHLNQPKIKYSQTATYVSRQYYFTAGYYFQLPNPAFELV
                     PSVFIVNDGATTQYLATGMVRYNKKIWGGVSYRISDAITGFAGIELYNGLKIGYGYDF
                     PVSEIRKGTSGSHEFMVSYSFDLGLGKSVTRYKSIRFL"
     gene            7023..8357
                     /locus_tag="PKK03_09110"
     CDS             7023..8357
                     /locus_tag="PKK03_09110"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNX84599.1"
                     /translation="MKKVTLLALALILILSSCGSYESGELTGVQGRKKFYEPEPFEMA
                     FIPAGSFVQGPSDQDPLRAMNSLSKTVTVEAFWMDQTEITNNKYRQFTYWVRDSILRT
                     RLGEEGIEGYEFFRTDEEGNVMEPNVLNWEEEIDMDDENVQAVMESMYYPPEERFNGN
                     KQWDTRLLNYSYFWVDYKQAAKARYNYKEQKYEGTVTDLEGNTTEVKDRSSFIMHHVV
                     NIYPDTLAWIRDFTYSFNEPWATLYFWHPGYDDYPVVGVSWIQARAFSVWRTNFMNEH
                     LRAQGIADVHNYRLPLETEWEYAARGGLDLSMYPWGGPYTRNYQGCFLANFKPLRGNY
                     IDDGAVYTIKVASFEPNEYGLFDMAGNVAEWTSCAYHPSSYIFTHDLNPDYEYNARPD
                     DPPVLKRKVIRGGSWKDIALFLQTSTRDYEYQDSTKSFVGFRNVRTYIGVNN"
     gene            8385..9251
                     /gene="gldL"
                     /locus_tag="PKK03_09115"
     CDS             8385..9251
                     /gene="gldL"
                     /locus_tag="PKK03_09115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HNX84600.1"
                     /translation="MGLSEIVHTSKWKGFMSKLYGWGASVVIIGALFKIQHYPMAGLF
                     LSVGLITEAIIFFFSAFEPLKEEPDWKLVYPQLDPNYSGEDIGFAAVGGGGGGYGYGG
                     GGGAGLSQFDKMLSDAGITPAVFDSLSQGLKKLTETTNNFNAMGDLAATTNEYAGTIK
                     KANESLMNLSESVRQTARSMTDTSNVYQAIGESSKTIETGGKSYHEKLEALNKNLSAL
                     NAAYELQLRGTNDHVKSAETIYKGMEGLMKDLTGSADDTKKYREQVAKLNENLSSLNN
                     VYGNMLAAMNVK"
     gene            9297..10940
                     /gene="gldM"
                     /locus_tag="PKK03_09120"
     CDS             9297..10940
                     /gene="gldM"
                     /locus_tag="PKK03_09120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HNX84601.1"
                     /translation="MSKGKETPRQKMIGMMYLILTAMLALNVSKEAVEAFRKVDMSLT
                     KTTANYIKKNDITYAAFDAAAAENPEKAGAWKTKAYEIKGRADELYNYIQDLKVEIIT
                     TAEGSDSEALLPDNQIDINKVKKIDENNVPSEILVGANQGGKGNDLKALIEDYREYLV
                     ETLEGKDASAERSILDILNTDNPQNLEKTGTEDWVTANFQTMPLVAAITMLSKMQVDV
                     RNAETDVLNFLYTQIDAGAFRFNYIVPTVTTNTSYVMQGNEYEAKVFVAATDTTQDLE
                     IFVGPYTTKTNPDGTPLYEPTSQATQLPIDESGRGVYRVRPGSVGEKSWGGVIRMKAP
                     DGSVRTYKFDQKYSVGMPNVVVSPTAMNVLYQGIQNPLDISVPGVGSDKLTVRMTNGD
                     ISKGKYKNYRGEYVAQPRTVGQNAQIIVSANIDGKVQSFPPVEFRVRRLPDPEARFAN
                     MKEGNVLKSVAAAQQVVTAVLENFEFDLTYTVTGFTVSVNDKGYEITAESNNNRLTDK
                     QKGLIGNLRAGQKLIIEKIKAVGPDGRTRDLNPIILKIN"
     gene            10959..>11151
                     /locus_tag="PKK03_09125"
     CDS             10959..>11151
                     /locus_tag="PKK03_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84602.1"
                     /translation="MKRTILAGLAGLLMCTGAMAQSFGDIYTKQMPDNQKVNYTYLNE
                     SDVIWSKRLYRIIDLREKMN"
BASE COUNT         2899 a         2907 c         2840 g         2505 t
ORIGIN      
        1 tactccctct gcctgtacgg attgccctgc tgctccctgt tcaccctgtc acagtcaaga
       61 tctacattga atccgggagg cgcttcaaac tgtgattctg ctatcactga gaaatccttg
      121 tcggccagca gcttctgcat aaagatcccg aacaccggaa gggccatgtt ggcaccctgt
      181 cccatcgaga gatcctcgaa atggattgcc tggtcctccc atcctgtcca gacaccggcc
      241 acaagatcag gcatgactcc cataaaccat ccgttggagt ggttctgcgt ggttccggtc
      301 ttccccccca tctggttggt aagcttgtag gtaagcctga gtctcacggc ggtaccgctg
      361 cttaccacac cctgcagcag cgaggtcatg aggtaggcat catcctcgct gataacctcg
      421 tctatgaacg gggtgaaggt ggagatgaca ttcccgttgc ggtcttctat ccgcgtaaca
      481 tagagcggcc gggtatacac ccccttgttg acaaaggttc cgtatgcccc caccatctcc
      541 tcgacggaga gatctgaggt accgaggaat atggagacaa caggatcaat atagctcctg
      601 attcccatac ggtgcattat gtcaaccacc gactgcggtg agaattgctt catcaaccag
      661 gctgatatgt agttttccga ctgggccagc ccccatttca gcgtcaccat cttgccgtga
      721 tactcctcag gacctgagct tctgggccgc catatcgtgt cattgacctc gaaggtgcgc
      781 tcaatgttct caacctcgta gcatggcgag taaccgtcct gcatggccag tgtatacagg
      841 aagggtttga tggtggaacc cacctgtttc ttctgctggg tgacagcatc atacttgaag
      901 tacctgaaat caggtccgcc gatatacgcc ttgacatgcc ccgtgtgggg gtccatggcc
      961 atgaatgatg atctcacgaa aaacttgtaa taccttatcg agtcaagcgg tgtcataacg
     1021 gtgtctctct ctccctgcca tgagaacagc ttcataggca cgggcgtgtt gaatgccagc
     1081 gttatcgagt cctcgggcac accggccctt ctcatgctct ggtacctgtc gctctgcctg
     1141 actgaccttg ccatgatctg gttcacctgt gccgtggaaa ggtcatttga gtagggggga
     1201 ttccggtaat tccttgccct cttgtcaaag accggctgaa ggtttttgga aaggtgctcg
     1261 gcaagagcct cttcagcata ggtctgcatc ttcgagttaa gggtggtata gattctcagt
     1321 ccgtcacggt agatattata tggtgagccg tcaggcttgg tgtttttgta gcaccagcca
     1381 tagagcggat cattctgcca gcgccagaga gcctcattgt aggcatcatc tgaaaagaac
     1441 ctgtctcgtt gcggttcctt gctgttcatt gttgacctta tatactctct gaagtatgtt
     1501 gccgggcccg tgttgtggct gtcctcgcga aggtcaagac ccagcggaag ctggctgact
     1561 gagtctgcca gggcacggga gatgtacccg tacctctcca tctgtgagat taccacattc
     1621 cgcctcttca gggcgctctc ctcattgcgg aggggattgt aggccacaga gttcttcagc
     1681 atgccgacca gcattgccga ctgctcgata tttagagaat caggggtggt attgaaatag
     1741 atgcgcgcag ccgaattgat gcccacggca ttatagatga agtcaaacac gttgagatac
     1801 atggtgataa tctcttcctt cgtgtagctg cgctcgagct tcactgccgt ctgccattcc
     1861 ttgaacttgg acaccgccag cttcgaggca cgcacaatgg ccggatcatt ggagagatcc
     1921 ctcggcagga aaaggttctt cgccagctgc tgtgtgatgg tgcttccacc tccggtgttc
     1981 tgaccaagca tgatagtctt gaccagaact cttgcaagac cacggaagtc aatgcccgaa
     2041 tgcctgtgga acctgatgtc ctcagtggcg ataagcgcat caatcacata cggtgaaata
     2101 tcttcgtaat cagtccatgt gcggttctgt atgtaaaact tgccaaggag gacattgtct
     2161 tccgaatata cctcggcagc aaggctgttg tcgggattct caagctcctc gaaggaaggg
     2221 atcgggccaa gcctgttggc ggagatcagc aagaacaatg tcagcaggaa cactgccggc
     2281 agagatatta ttacccagaa ccagataatg tttttccgga attttttatc cctcatgata
     2341 ccctcattat aaggccgcta atttaatgat taatacagaa tttttctcaa aattttgaag
     2401 ttggcgccct tattcattta ctttgccctg tttttaaaaa aactcggatg actgataatc
     2461 tggtaatagt tgaatctcct gccaaagcaa agacgataga aaaatttctc ggaaaagatt
     2521 acaccgttgt gtcgagcttc gggcatatcc gtgatctcgt acgaaacaac ctgggtgtgg
     2581 aggttgacaa caatttcgca ccggtatatg aaatacctga agacaagaag aaggttgttg
     2641 ccgagcttcg caagcttgct gacaaggcca caacagtatg gattgcttcc gatgaggacc
     2701 gtgaggggga ggcaattgca tggcacctga tctccgttct cgggcttgac ccggcaacaa
     2761 cgcgccggat tgtattccat gagattacga aagatgccat tacccgtgcc gtggaatcgc
     2821 cgcggcaggt agacatgaac ctggtcaacg cccagcaggc acgccggata cttgacaggc
     2881 tggtaggctt tgagctttca cctgtgctgt ggaaaaaggt acagccttca ctttctgccg
     2941 gaagggttca gtctgtagca gtgcgcctgc ttgttgaccg tgaacgtgaa ataattggct
     3001 tcgttgctga ttccgccttc agggtaaccg gcacattcag cggaacaaac agtgaaggga
     3061 acgaagtgca gctcaaggca gagtgcccga agaaattcag cacggaggaa gatgcaagac
     3121 tcttcctgga aaagtgtgac gcctctgcat tcacagttga aaatatagtg gtcaagcccg
     3181 gcacccgctc gccggcgcct ccctttacca cctccaccct ccagcaggag gcctatcgca
     3241 agctcggttt ctctgtggca cagaccatgt cagtggcaca gaaactgtac gaggccggaa
     3301 agatcacata catgaggacc gactccacca atctctcctc gctggcactc aacaccgcca
     3361 gggaagtgat taccaatgag tacggtgcag cctactcgcg cacccggcag ttcaagaccc
     3421 attcgcgcgg tgcccaggaa gcacatgaag ccatcaggcc aacctacttc gacaggaagg
     3481 aggttaccgg caacaacaat gagaagaagc tctatgaact gatatggaga agagcgatag
     3541 cctcccagat ggccgatgcc agggtggaaa agacaaccat aaccattggc atgtctgctt
     3601 cacccgtcag ctttacggca accggtgaag ttatcctctt cgacggcttc ctgaaggttt
     3661 ataccgaatc atatgacagc aacgcctccg aggatgaaaa ggcaatactg ccccgcgttg
     3721 accaggggat gaaactcaca cccggaacca tcaccgccct ccagcgattt acttcacccc
     3781 ctccgcggta tacggaagcg agcctggtga agaagctcga ggaactcggg ataggaagac
     3841 cgtcgactta cgcgcctatt atatccacaa tacagtcaag gggttatgtg gcccgtgagg
     3901 acaggcccgg ggagaagcga acccttgccc agctcgccct ctccaaggga aaaataacca
     3961 ggagcgagaa gaccgagata gccggcaagg agaagtcaaa actcttcccg aaggatattg
     4021 gcatgattgt gaacgacttt ctgattgcca attttcctga catcctcgat tacaacttca
     4081 ctgcaggtgt ggagaaggag tttgatgaga tagcctcggg cgaggtgaag tgggaaaaga
     4141 tgatctccac attttacgga cccttccgga agaccgttga cgaatctctt gccacgcggg
     4201 cgaagatgac cggcgcgcgg cagcttggca cagacccggc aacgggcctg ccggttgcag
     4261 tgaagatgag ccgtttcggg cccgttgtac agctgggcga ggcctcggag gaacagaagc
     4321 cccggttcgc caacctcaga cgtgaccagc tgcttgaaac cattaccctt gaggaggccc
     4381 tcgccctctt cgcgctgccg cgcgccgtgg gtaccttcga ggatagtgag atgaccgtgg
     4441 gggtgggtaa attcggacca tatgtcaaac atgcaggcaa gttctactct ctgaagagag
     4501 gcgttgatga tccgtataca atcaccggag acagagccat tgaactgatc acggaaaaga
     4561 gggaaggcga caagcagaag gtcatcagcg agctgggtga tatcatggtg ctcaacggac
     4621 ggtacggtcc gtacatcatg catgacaaaa ccaactaccg catcccgaag ggaactgacc
     4681 cccataagct taccagggat gagtgcctgg caataataga gaagggaagc aagaccaaaa
     4741 ccaccagggg aaggaagaag aagtgacctt atataatatg attgacctga accagtatat
     4801 tgatcccgtc tgccttgaca agccgggggt ggagccgctt aaggggtctt ctgcctttcc
     4861 gcacaatatg ttggtgaata ccgggaatga gcccctgggt gacctcagcg gattcgcagt
     4921 agccatactg ggtgtgccgg atgaccgctc ttcagtgaat acaggagcgg cacaggcgcc
     4981 tgatgctgtc aggaggagtc tctactcctt ctccaggctg ccgggcaggc tgaaggtcac
     5041 tgacctgggc aacttcaggc cgggggcctc cttcgatgac accatagcag gccttcgcga
     5101 cgtgcttctc cagcttctgt cacagaacat cgttccggtg atcatagggg gaacaagctc
     5161 cctgatgccg gctgttgaca gggcgctctc tgcaggaaaa cagaaatggt caatggtgtc
     5221 ggttgattcc cgtatagact tcaccgccga gaagagaccg cccgactcgc tcaactggat
     5281 gaatgacctc atctatcgca gcggcagctg cctgtcacac ctctcagcca tagggcacca
     5341 gacctatctc actgaccagc aggtggtgaa caggttcaac cgccgtcatt atgacatgat
     5401 gcgcattggc gaggttcgct ccgcggtaca tgagaccgag cccctcttcc gtgacgcctc
     5461 cgccgcactg tttgatatcg gatcagtaag gcagtcagac gctcccggaa cattccttcc
     5521 gtcagccaat ggtttctacg gcgaggagat atgcctcctt gcccggtacg ctggcctctc
     5581 agataacctg aagatttttt ctgtgacgga ggtcaacccg gcccttgaca caaggatgca
     5641 gacatcgcac ctggcagcgc agcttgtctg gttcttcctc gaaggcttct ctcagaagca
     5701 gtacgaagtc tcttacctca gtgaccagac gaacagcagg ttcacccgtt atcatgtaac
     5761 cctcagtgac ctggagagtg aagccatctt cataaagagc accatcaccg agcgctggtg
     5821 gatggagatc cgtaacaccc gtggcgaacc gcagtatctt gcctgctcat atgaagacta
     5881 cctgatggct aaccgtgatc aggtacccgt caggtggtca cgcggaatgc tccgccacgg
     5941 ccactgattt ttggcctcaa ttttgtggca tgtatatttt tttatttatt ttacccgtta
     6001 tatgtaaggg tttacaggca caccccggct atgaagaaaa caaggatatt aatcctggca
     6061 ttgttactcc cggcaggcat cgtctgcggt cagcaggacc cggtgttcag ccactacatg
     6121 ttcaataccc agacatataa tcccgggttc tcgggcatgt cggggatgat caccgcctcg
     6181 gcacttacgc gtcagcagtg ggtgggtttc cccggtgcgc cctcaaccat gcttttcaac
     6241 atcaatatgc cgttcagcct cttcggtttc cgcagtggcg ccggcctgct tgtggaagca
     6301 gacaaatacg gattcagcaa tgatattgac atcactcttt cctattcagg cctgttttcg
     6361 gtcggaaccg gtaccctcgg agccggtttc agcattgggg tgctcaacaa gactgtttcg
     6421 ccggaatggt tcatcccttc gggcccgggc catacgcctc cgacaggtga ccctctgatt
     6481 cctgagaatg atgaatcttt tcttgcagct gacttttccg ccggggtata ctaccagggg
     6541 ctcaactact atgccgggct gtcagtcaca cacctgaacc agccaaaaat aaaatattct
     6601 caaacagcca cttacgtcag ccgccaatac tacttcactg ccggttacta cttccagctg
     6661 ccaaaccctg cctttgagct tgtcccctca gtttttatag taaatgacgg cgccacaaca
     6721 caatatttag ccacaggtat ggttagatac aataaaaaga tatggggtgg cgtttcttac
     6781 aggataagtg acgcaatcac aggattcgca ggcatagaat tgtataacgg tctaaagatt
     6841 ggatatggat acgacttccc ggtatcggag atcagaaaag gaaccagcgg ctcacatgag
     6901 ttcatggtct cttacagctt cgaccttggt ctcgggaaaa gcgttaccag atacaagagt
     6961 atccgtttcc tttagtggac aggaaaaaat aaaattactt gcatatttaa attaatgagt
     7021 atatgaaaaa ggtaaccctt ttagctcttg cgttgatctt gattttaagc agctgtggct
     7081 cttacgaatc aggcgagttg acaggcgttc agggaaggaa gaagttctat gagccggagc
     7141 cgtttgagat ggcattcata ccggccggtt cctttgtaca gggaccaagt gaccaggacc
     7201 ctctgcgcgc aatgaacagc ctctccaaga cagttacagt ggaagcattc tggatggacc
     7261 agacagagat caccaataat aaataccgtc agttcaccta ctgggtgcgt gactccatcc
     7321 tcaggaccag gctgggagag gaaggaattg agggttacga attcttcagg actgacgaag
     7381 agggcaatgt catggaaccc aacgtgctga actgggaaga ggagattgac atggatgatg
     7441 agaacgtcca ggccgtgatg gaatcaatgt attatccccc cgaagagaga ttcaacggaa
     7501 acaagcaatg ggacacccgg ctcctgaact actcctattt ctgggttgac tacaagcagg
     7561 cagccaaagc caggtacaac tacaaggaac agaagtacga gggtaccgta actgacctcg
     7621 agggcaacac caccgaagtg aaagaccgtt catcattcat tatgcatcac gtggtgaata
     7681 tctatcctga cacccttgca tggatcaggg acttcaccta ttcattcaac gagccgtggg
     7741 cgacactcta tttctggcat cccggttatg atgactaccc ggttgtggga gtatcatgga
     7801 tccaggcacg cgcattcagc gtgtggcgta ccaacttcat gaatgagcac ctgcgtgcac
     7861 agggcatcgc cgacgtacac aactaccgtc tcccgctgga gaccgagtgg gaatatgccg
     7921 cacgcggcgg acttgacctc tcaatgtacc catggggcgg tccctacaca cgcaactacc
     7981 agggatgctt cctggcaaac ttcaaaccgc tgcgcggtaa ctacatcgac gacggagccg
     8041 tttacaccat aaaggtggct tccttcgaac ccaatgaata cggattgttt gatatggcag
     8101 gaaacgtggc cgaatggact tcgtgcgcct accatccctc atcatatatc tttacccatg
     8161 acctcaaccc cgactatgaa tacaacgccc gcccggacga tccgcccgtg ctcaagcgca
     8221 aggtcatcag gggaggctca tggaaggata ttgcactgtt ccttcagaca tcaacaaggg
     8281 actacgaata ccaggactca acaaaatcat ttgtcgggtt ccgcaatgtg agaacttaca
     8341 tcggagttaa taactaatac cgtctaactt taatacataa tcatatgggc ctctcggaaa
     8401 ttgttcatac tagtaagtgg aaaggattca tgtccaaact ttacggctgg ggtgcttcag
     8461 tcgtgatcat cggtgcactc ttcaagattc agcactatcc gatggctgga ctattcctct
     8521 ctgtgggtct tatcacagaa gcaatcatct ttttcttttc ggcgtttgag ccgctcaagg
     8581 aggagccgga ctggaagctt gtctaccccc agcttgatcc gaattactct ggcgaggata
     8641 tcggttttgc agctgtcggc ggcggtggcg gcggttatgg atatggcggt ggcggcggtg
     8701 ccggcctctc gcagtttgac aagatgctgt ctgacgcagg aatcacacct gctgtgtttg
     8761 acagcctcag ccagggactg aagaagctca ccgagaccac caataacttc aacgcaatgg
     8821 gtgacctggc agctaccacc aatgagtatg ccggcacaat aaagaaagca aatgagtctc
     8881 tcatgaacct ctccgagtct gtcaggcaga ctgcacggtc aatgactgat accagcaacg
     8941 tctaccaggc tatcggtgag tcttcaaaga ccatcgaaac cggaggcaaa tcataccatg
     9001 agaagctcga ggctctcaac aagaaccttt ccgccctcaa tgcagcatat gaactgcagc
     9061 ttcgcggcac aaatgatcac gtgaagagcg ctgaaacaat ctataaggga atggaaggtc
     9121 tgatgaaaga cctcaccggt tcagctgatg acacgaagaa atatcgtgaa caggtggcca
     9181 aactcaacga gaacctcagc tcacttaata acgtctacgg caacatgctt gctgccatga
     9241 atgtgaagta atgttgagtt catcaatata tacgataaca tctaaactat ccgcaaatgt
     9301 ctaagggaaa agaaaccccg cggcaaaaga tgatcgggat gatgtacctc atcctgacag
     9361 caatgcttgc tcttaacgtt tcgaaggagg cagtcgaggc tttcagaaaa gttgacatga
     9421 gccttaccaa gactaccgcc aattatatca agaaaaatga tataacctat gcagcttttg
     9481 atgcagcggc ggcagagaac cctgaaaagg cgggagcctg gaagacaaag gcctatgaga
     9541 ttaagggacg tgccgatgaa ctctataatt acatccagga tctgaaggtt gagatcatta
     9601 ccacggctga aggttccgat tcggaagcac tccttcccga taaccagata gatatcaaca
     9661 aggtaaagaa gatcgacgag aacaatgtcc cttctgaaat cctcgttggt gcaaaccagg
     9721 gcggaaaggg aaatgacctt aaggccctga tagaagatta cagggaatac ctcgttgaaa
     9781 ccctggaagg caaggatgcc tcagcagaac ggtcaatact tgatattctc aatactgaca
     9841 accctcagaa ccttgagaaa acaggtactg aagactgggt cacggccaat ttccagacca
     9901 tgcctctcgt tgctgccata accatgcttt caaagatgca ggttgacgta cgcaatgcag
     9961 agactgacgt tctgaacttt ctctataccc agattgacgc cggtgcattc aggttcaact
    10021 atatcgtccc cacggtcaca acgaatacct cttatgtaat gcaggggaat gaatacgaag
    10081 ccaaggtatt cgtggcagcc actgacacca cccaggatct cgagattttc gtgggcccat
    10141 atacaacgaa aaccaatcct gacggaactc ctctctacga acccaccagc caggccacac
    10201 agctgccgat tgatgaatca ggcaggggag tttacagggt tagaccaggc tcggtaggtg
    10261 agaaatcatg gggaggcgtc ataaggatga aagccccgga tggcagcgta cgcacttaca
    10321 aatttgacca gaaatattcc gtgggaatgc cgaacgtggt agtgtcaccc accgccatga
    10381 acgtcctcta ccagggaata cagaaccccc ttgacatttc agttcccggc gtcggatcag
    10441 acaaactgac cgtccgtatg accaacggtg acatcagcaa gggaaaatac aagaattacc
    10501 gcggtgaata tgtggcacag ccacgtaccg tgggacagaa tgcccagata atcgtgtcag
    10561 ccaacataga cggcaaggtg caaagcttcc cgcctgtgga attcagggtc aggagattgc
    10621 ccgaccccga ggcacggttc gcaaatatga aggaaggcaa tgtactcaag agcgtggccg
    10681 ccgcacagca ggtggtgacc gcagtgctcg agaactttga gttcgacctt acatatacag
    10741 tgacaggctt tacagtctct gtcaatgaca aaggctatga aatcacagcc gaatcaaata
    10801 acaacaggct taccgacaag cagaaaggcc tcataggaaa cctgcgcgcc ggacagaaac
    10861 tcataataga aaagatcaag gctgttggcc ccgatggcag aaccagggac ctcaacccga
    10921 ttattctcaa gattaactaa caacagaaga tacaaaccat gaagaggaca attctggcag
    10981 gcctcgcagg cctcctgatg tgtacaggcg caatggccca gtcgttcggc gacatctata
    11041 ccaagcagat gcccgataat cagaaggtta attacaccta tctgaatgag tcagacgtga
    11101 tctggtcaaa aagactatac aggataatcg acctgaggga aaagatgaac c
//
LOCUS       DAOPXU010000089        11030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_6805_length_11030_cov_12.248656, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000089 DAOPXU010000000
VERSION     DAOPXU010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11030
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6805_length_11030_cov_12.248656"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1213)
                     /locus_tag="PKK03_09130"
     CDS             complement(74..1213)
                     /locus_tag="PKK03_09130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX84603.1"
                     /translation="MIISTDTTHLILLGLLLAMFIIQAWYWLGYYRRAAFAKVPEIAP
                     GTPLPPLSVVICARNEAENLSRFLPDVLKQDYPSFEVVVVNDCSEDNSFDILGDLMTR
                     YPHLKVSMIQKDPGFTHTKKLAMLIGIKAAKNDLLVFTDADCRPASPQWLRHVAAAAT
                     CEKTDIVLGYGAYNVEKGLLNSYIRYETMFIAMQYFGMAMAGTPYMGVGRNLAYRRTF
                     FFGKGGFGPHNHIMSGDDDLFVNRTATSENCSLMLAHNSFTLSVASKTIREWVKQKKR
                     HLTAAAHYKNRDKLRLFLEPFSRVSWYGLLIAMLVMLVSWPVVLFLALSRVVMRAVIL
                     RKAAAVFNERHLWFVSLFFDIFAPFLTAFLYLTNRRKGKGRETWK"
     gene            1368..2495
                     /gene="tgt"
                     /locus_tag="PKK03_09135"
     CDS             1368..2495
                     /gene="tgt"
                     /locus_tag="PKK03_09135"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNX84604.1"
                     /translation="MFTIESRDGATSARAGILRTAHGDIQTPIFMPVGTAANVKGVFH
                     RDLKQEIDAPVILANTYHLYLRPGTEIIRQAGGVHRFMAWDRAVLTDSGGYQVFSLAA
                     NRKLTEEGAIFRSHIDGSKHLFSPESVIDVQRQIGADIIMAFDECAPYPCDHRYALKS
                     MEMTHRWLDRCVARMASTEPLYGHVQLLFPIVQGAVYGDLRKISAEKCASSGMPGNAI
                     GGLSVGEPAEVMYEMIEVVNEILPADKPRYLMGVGTPANILEGIARGIDMFDCVMPTR
                     NGRNGMLFTRDGIVNIRNRKWATDFSPIDAGSHSEISRTYTRAYLRHLVISGEMLGAQ
                     IATIHNLAFYLSLVREARERILDGTFGSWKEETVRKISVRV"
     gene            2453..3592
                     /locus_tag="PKK03_09140"
     CDS             2453..3592
                     /locus_tag="PKK03_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNX84605.1"
                     /translation="MEGRNSSKNISEGLKKLSPGILDWYIIRQFLGTFLFSLVLILGI
                     AVIFDFSEKIDDFIQKQAPMRAIIFDYYLNFIPYFATLFAPMFVFISVIFFTSRMAVN
                     TEIIAMLNSGMSFRRLMVPYLISAGFIAFCAFLLQNSVLPHANVARLDFEEKYYRSSD
                     FRRVNVSSVHRQVYPNVYIYMEQYNRISQSGRNFSMEKFNDQGMLESKLTANMIRWDS
                     TSGKWGAWWYYIRETDSLGERISSGRRIDTVLNVGPDDFTRSPEFVFTMTHNELIDYI
                     HVLKAQGSDEIKLYLNEKHRRYASPFAFFILTIIGVSLSSRKIKGGIGMQIGLGLMLT
                     FSYILFMQFSSQFSLKGDLNPAIAMWIPNIIYTFIAAGVYYTAPK"
     gene            3681..5228
                     /locus_tag="PKK03_09145"
     CDS             3681..5228
                     /locus_tag="PKK03_09145"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNX84606.1"
                     /translation="MMALEKKIRELKEKREKMLLGGGEEAIVKQKAMGKNTARERIMA
                     ILDAGTFNEYDMFVEHDARDFGMDKKNLPSDGVVIGTGTVHGNPVAIFAQDFTVAGGS
                     LGLMHSRKITKIMDHALKMRIPLIGINDSGGARIQEGVNSLAGYGEIFFRNTISSGVI
                     PQISVILGPCAGGAVYSPALTDFVFVVDNISKMFITGPEVIKTVLGEEISMEALGGAR
                     VHCETSGNAHFFSSSEEECFSQIKRLISFIPRNNEMKAAVAETLPPEAGYDIEKIVPG
                     DPTLPYDVREVIRAVADSSDFFEIQEMYARNMVIGFGRLGGRTVGFVANQPLEMAGVL
                     DVDSSDKAARFIRYCDAFSIPLVTLVDLPGYLPGVDQEHAGVIRHGAKVLYAYSEATV
                     PKLTVILRKAYGGGYIAMSSRHLRADFVFAWPTAEIAVMGPEGAANIIFRKEITSAAN
                     PDEMRRQKVDEYREKFANPYVAASRGYVDAVIEPSETRRLLLHGLEVSENKDVQMPWK
                     KHGIPPF"
     gene            5251..5565
                     /locus_tag="PKK03_09150"
     CDS             5251..5565
                     /locus_tag="PKK03_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HNX84607.1"
                     /translation="MEKKNTDRLIIDHTTYSTRLSRRFTSRKPYEPPVPGRIQSFIPG
                     TVVEVLVKTGDAVKEGDDIVILDAMKMKNRLKSHIDGKVMAVNVSPGDRVTKGAVLVE
                     IA"
     gene            complement(5586..6407)
                     /gene="ispE"
                     /locus_tag="PKK03_09155"
     CDS             complement(5586..6407)
                     /gene="ispE"
                     /locus_tag="PKK03_09155"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNX84608.1"
                     /translation="MVVFPKAKINIGLRIVEKRPDGYHNIETFFYPVDLADALEFVVR
                     KDNRRNDSLRVTGLMKEADPDNNLVMKAVKIMRQSFDFPGIRIHLHKSIPVGAGLGGG
                     SSDAAGMLKALNRYFGFGLNAEELRSFAGLIGSDAPFFIDGTPSFAEGVGNILSEFPL
                     DLRQYRIVILYPDTNISTAEAYAGCVPCPTGLSLRQLLNLPVSQWRGRVVNDFELNIF
                     RLYPQVGRLKVALYEAGALYASMSGSGSAVYGIFDSEPELPRELSRYRLTTAMLE"
     gene            6620..8143
                     /gene="dnaB"
                     /locus_tag="PKK03_09160"
     CDS             6620..8143
                     /gene="dnaB"
                     /locus_tag="PKK03_09160"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNX84609.1"
                     /translation="MAQGTSKSRQDKKQSVVIIPEGGRIPPQAAEMEEAVLGAAMLEI
                     EAARMVVDMLRPEVFYSAAHQKIFASIIELNKRDQPIDLLTVTQELKRTGELEEAGGA
                     VYLTELTSRIVSAANIEYHARIVLQKYIQRELIRAATMIQNLVYEDTLDVTELLDRSE
                     DELFRIAEGNIKHEVAPIHEVIKQTLAEIEELGKKEDALVGVPSGFTKLDRLTSGWQR
                     SELVIIAARPSMGKTAFALTMARNMAIDHGRKVAIFSLEMSAIQLVNRLIVSETEIPG
                     EKIKNGKLSPEEWSLLESRIKSLTEAPIFIDDTPAISVFELRAKCRRLKAQGKLDVVV
                     VDYLQLMTGPPDAGSREQEVSSISRSLKSIAKELAVPVLALSQLNRSVETRGGNKKPM
                     LSDLRESGAIEQDADMVVFIHRPEKMGVLQFEDGQSTKGHADIILAKNRNGPTDEVLL
                     YFKEDRASFVEPDDFGPDVAFPDIPSGGVFTMGSKMNDDISSDYSDFSPDDNGRRPF"
     gene            complement(8269..>11030)
                     /locus_tag="PKK03_09165"
     CDS             complement(8269..>11030)
                     /locus_tag="PKK03_09165"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNX84610.1"
                     /translation="GRRQIPEALSSLPYITNNGLVPGTRILILVAFLLLPAISVNAQV
                     LRGRITDSSGTPMPSASVYIAELRQGTTSNNEGWYEISLPPGNYNVNYQFLGYIPVTR
                     NIAVTGEGVAADITLTEQLFEIPPVRVSASGKDPAYFIMRKAIGMAPYHLNQVKMWKA
                     EVYIRGGGSVDRIPEMLKRRMKIDSNGDDLKVGKYYFTESVNMITFTAPDKYEHRVIS
                     ANSSEDLTQGQASPMDFIEASFYQPVLVEMAISPLAPNAFSHYNFTFLGSSSQGDNVI
                     DKISVTPRRKSQQLFTGVIYIVEDLWAIHSLDLTNDNMAGRIRVRQVYTPVEDGIWMP
                     VSHEFNADISIMGVKARAAYTSAVRYLDVEPDRILRRPAAYETAITAGAEEGPAERPK
                     TSAGREIEALLAKDDLTARDMSRLARLNEKNVRTTRGKPPLEIEDKTTMIVEDGATGR
                     DSAYWAEVRPIPLTREESASLATAPAAKGKLAVRDTSTLTITIGGRSQPGSDSAAVSD
                     SAGMKTSPVSRFIRDLTGGKRWQLSKNTFLEFDGLADLKSFSYNTVDGFVAGTGMSLS
                     ARSGKTGRFTIAPSARYAFSREKLLWNVTANMLYDPMHAGNIYIRAGSQSDEFSASGV
                     NQLVNTVSSLFFRENIMRLYGSQYIIGGHRIDVVNGLTLDIQGKYEKRDPLPNNSSFS
                     FFRREKPWSQNIPDNPYASAAEGYDYEPPVSHRHLSLESSLTWTPRQRYRISGGARVS
                     AGSDYPTFTLTWRHGYGYNDTLSGSYDLLAAEVNRTDRFGAMNEFRWRIRAGGFLNSE
                     NARMQDMHFFNTQSSPVLLNFYEDAFYLKPCFSVSTRKAFAEAHLRYTTPTLLLKRLP
                     GLSRTLIRENIGISALWTPEAGNYYEAGYSLSEVLLVGTVGVYAGFTGTSFESAGIRV
                     ILRLE"
BASE COUNT         2577 a         2738 c         3051 g         2664 t
ORIGIN      
        1 gccctgctgt cgccgtgcag tgcacggtcg atgaccttga ggtcatactg cgctttatcc
       61 gataggccgc tttctacttc catgtctccc tgcccttacc ttttcttctg tttgtcaggt
      121 aaaggaatgc cgttaagaat ggggcgaaga tatcaaaaaa gagcgaaaca aaccataagt
      181 gccgctcatt gaaaacagca gctgcctttc tgagtatcac cgctctcatg acaacccttg
      241 acagggcaag gaagaggaca accggccacg agaccagcat caccagcatg gcaatcagca
      301 gtccgtacca tgataccctc gagaagggct cgaggaacag ccggagcttg tcccggttct
      361 tgtaatgcgc cgcggcagta aggtgtcttt ttttctgttt cacccactcc cggatggttt
      421 tggaggcaac agaaagagtg aatgagttgt gggccagcat caggctgcag ttttcagagg
      481 tggcagtgcg gttcacgaag aggtcatcgt cgcctgacat tatgtggtta tggggcccga
      541 aaccaccttt cccgaagaag aatgtacgcc ggtatgccag gttgcggccc acccccatgt
      601 atggtgttcc tgccatggcc attccgaagt attgcattgc aataaacatg gtttcatacc
      661 tgatatagct gttcagcagt cccttttcta cgttgtacgc tccgtacccc agtacaatgt
      721 cagtcttttc acatgttgct gctgcagcca cgtgacggag ccattgcggc gaagcaggcc
      781 ggcagtcggc atcagtaaat accagcaggt catttttagc ggccttgatg ccgatgagca
      841 tagccagctt cttggtgtgt gtgaaacctg gatccttctg gatcatggag actttcaggt
      901 gcgggtaccg ggtcataagg tctccaagga tatcaaagga gttgtcctcg gagcagtcgt
      961 ttaccaccac cacctcgaag gaagggtagt cttgtttgag aacgtcgggc aggaacctgc
     1021 tgaggttttc tgcttcatta cgggcgcata ttaccactga caggggcggg agcggggttc
     1081 cgggagcaat ctccgggacc ttggcgaagg cagctctcct gtaataacca agccagtacc
     1141 aggcctgtat aataaacatg gccagaagca gcccgagaag tataaggtgc gtcgtatcag
     1201 ttgaaatgat catgtgaggt gatattgtgc ggagcaatat tataaagaaa tggaaaacag
     1261 tgggaataat cttttcaaca atcatggtcc ggattcttca aattgataac tgcctgttaa
     1321 aaatcaggct gagaaagctt acttttgtga ccatcacaga gaagcggatg ttcactattg
     1381 agagcagaga cggagccaca tcggctcggg caggtatatt gcgtacggca catggcgaca
     1441 ttcaaacgcc aattttcatg cctgtgggaa cggcggcaaa cgtcaagggg gtttttcacc
     1501 gtgacctgaa gcaggagata gatgcaccgg tcatccttgc caatacctat catctatacc
     1561 tgcgtccggg cactgagata atcaggcagg caggaggcgt gcaccggttc atggcgtggg
     1621 acagggcagt gctgactgac agcggcggtt accaggtttt ctcactggct gccaacagga
     1681 agctcacgga ggagggcgcc atcttcaggt cgcacataga cggttcaaag catctttttt
     1741 cacctgaaag cgttattgac gtacagcggc agatcggggc agacatcatc atggcttttg
     1801 atgaatgtgc tccgtatccc tgtgatcaca gatatgccct gaaatcaatg gagatgactc
     1861 accgctggct tgacaggtgc gtggcacgaa tggcttcaac agagccgctc tacggacatg
     1921 tgcagctgct gtttccgatt gtgcaggggg ctgtttacgg ggatctgcgt aaaatatccg
     1981 cggagaaatg tgcttcaagc ggcatgcccg gaaacgccat cggaggactc tctgtaggtg
     2041 aaccggctga ggtgatgtac gaaatgatag aggtggtgaa cgaaattctc cctgcagaca
     2101 agccccggta cctgatggga gtgggaacac ctgcaaacat tcttgaaggg atcgcgcgcg
     2161 gcattgatat gttcgactgt gtcatgccta cacgtaacgg acgtaacgga atgctgttca
     2221 cccgtgatgg catagtaaac atcaggaatc ggaaatgggc aaccgacttc tccccgatag
     2281 atgccggttc acattctgag ataagcagga catataccag ggcatacctc cgccacctgg
     2341 taatctcggg cgagatgctc ggtgcgcaga tagcaaccat tcacaacctt gcgttttacc
     2401 tcagcctcgt cagggaggca agggaaagga tacttgacgg tacattcgga tcatggaagg
     2461 aagaaacagt tcgaaaaata tcagtgaggg tctgaaaaag ctgtctcccg gtatacttga
     2521 ctggtatatc atcaggcagt tcctcgggac attcctcttt tcgctggtgc tcatcctggg
     2581 catagcggtg atatttgact tttccgagaa aattgacgac ttcattcaga agcaggcgcc
     2641 gatgagggcg atcatcttcg actattatct taatttcatt ccatacttcg ccaccctctt
     2701 tgccccgatg tttgtcttta tctcggtgat cttctttact tcgaggatgg ccgtcaacac
     2761 tgagatcata gccatgctta acagcggcat gagtttcagg aggcttatgg tgccgtatct
     2821 tatatcggcg ggtttcattg cgttctgcgc tttcctgctg cagaacagcg ttctgccgca
     2881 tgccaatgta gccaggctcg acttcgagga gaagtattac cgcagctctg acttcaggag
     2941 ggtgaacgtc tcatcggtgc accggcaggt ataccccaac gtgtacattt atatggagca
     3001 gtataacagg atctcccaga gcggccgcaa cttcagcatg gaaaagttca atgaccaggg
     3061 gatgcttgag tcaaagctga ctgccaacat gatcagatgg gacagcacct ccggcaaatg
     3121 gggcgcctgg tggtactata tcagggaaac tgacagcctc ggtgaacgga tcagctccgg
     3181 acggcggata gacaccgtgc ttaacgtggg gcctgatgat ttcacaaggt cgcccgagtt
     3241 tgtctttaca atgacgcata atgaacttat cgactatatc catgtgctta aggcacaggg
     3301 ttctgatgag atcaagcttt acctgaatga gaagcaccgg cgttatgcct cgccgttcgc
     3361 gttcttcata ctgacaataa tcggagtctc actatcatcg cgcaagatca agggagggat
     3421 cgggatgcag atagggctcg ggctcatgct gacattcagc tatatactct tcatgcagtt
     3481 ctcctcgcag ttttcgctca agggcgacct gaaccctgcc attgcaatgt ggataccgaa
     3541 tatcatatac accttcattg cggcaggggt ctactacacc gctccgaaat agcacatttt
     3601 cgacaatagt gtgcatattt gttttttgct taaattgccg tgcttttttt tgcaagtagt
     3661 tactgtaata aacaatattg attatggcac tggaaaagaa gatacgcgag ctcaaggaga
     3721 agcgcgaaaa gatgcttctt ggcggcggcg aggaagccat cgtgaaacag aaggccatgg
     3781 ggaagaacac tgcccgtgag cgcattatgg ccatccttga tgccggcacc ttcaatgaat
     3841 atgacatgtt cgtggagcac gatgcccgtg atttcggaat ggataaaaag aacctgccca
     3901 gcgacggtgt ggtgataggt accggcaccg ttcacggcaa cccggttgca atttttgcgc
     3961 aggattttac tgttgcaggg ggttctctcg ggctgatgca ttcgcgaaaa atcaccaaga
     4021 tcatggatca tgccctgaag atgaggatac cgctcatagg aataaatgac tctggtggtg
     4081 cacgcatcca ggagggggtc aattcactcg ccggttatgg tgagattttc ttcaggaaca
     4141 ccatctccag cggggtcatt ccacagatct cggtgatcct gggaccatgc gccggaggtg
     4201 cggtatattc gccggccctg accgacttcg tatttgtggt tgacaatatt tcgaaaatgt
     4261 tcatcaccgg tcccgaggtt ataaagaccg ttctgggcga ggagatatca atggaggcgt
     4321 tgggaggcgc ccgcgtgcac tgcgagacat cgggcaacgc ccatttcttt tcttcctctg
     4381 aggaggagtg cttcagccag atcaagcgac tgatctcctt cattcccagg aacaatgaga
     4441 tgaaggctgc cgttgctgaa acactgccgc ctgaggcggg gtatgacatt gagaagatag
     4501 tgcctggtga cccgacgctc ccatatgatg tcagggaggt tatcagggcg gttgctgaca
     4561 gttccgattt cttcgagatt caggagatgt atgcccggaa catggtcatc ggcttcgggc
     4621 gtctcggggg ccgtaccgtg ggttttgtag ccaaccagcc gctggagatg gccggggttc
     4681 ttgacgttga ttcatctgac aaggctgcga gattcatcag gtactgtgat gcattcagca
     4741 tcccgctggt gacccttgtt gacctgcccg ggtatctgcc cggtgttgac caggagcatg
     4801 ccggcgtcat ccgtcacggt gccaaggtgc tttacgccta cagcgaagcc actgttccca
     4861 aacttacggt gatactgcgc aaggcttatg gcggaggtta tattgccatg agttcgcgcc
     4921 atctgcgcgc cgacttcgtc tttgcatggc ctacggccga aatagctgtg atgggcccgg
     4981 agggagctgc caacatcatc ttccgcaagg agatcacttc tgctgccaat cctgacgaga
     5041 tgcgccgcca gaaggttgac gagtatcgcg aaaagttcgc caatccttat gtggctgcct
     5101 cccggggata tgttgatgcg gtaatagagc cgtcagagac tcgccggctg ctgctgcacg
     5161 gacttgaggt ttctgagaac aaggatgttc agatgccctg gaaaaagcat gggatcccgc
     5221 ccttctgatt aatgcccgaa agagactgca atggaaaaga aaaatacaga caggctcata
     5281 atagaccaca ccacctacag caccaggctg agcaggcggt ttacatcacg caagccttat
     5341 gagccgcccg taccgggaag aatacagagt ttcataccgg gaacggttgt tgaggtgctg
     5401 gtgaaaaccg gtgatgccgt gaaggagggt gacgatatag tcatcctcga tgcaatgaag
     5461 atgaagaaca ggctcaagag ccacattgat ggcaaggtca tggcggtgaa tgtcagtccc
     5521 ggcgacaggg tgaccaaagg cgcggtgctg gtggagatag cctgagtcag ataatggcag
     5581 gcggctcact caagcatggc tgtggtgagc ctgtaacgcg acaactcgcg cggcagttcc
     5641 ggctcgctgt cgaagattcc gtatacggcg gatccgcttc cagacatcga tgcatataat
     5701 gcccctgcct cgtagagtgc cactttaagt ctgcccacct ggggatacaa cctgaagata
     5761 ttaagttcga aatcattcac cacccttccc ctccactggc tgacgggcag gttgagcagc
     5821 tgtctgagtg agagccccgt aggacagggt acgcatcctg cataggcctc agccgtgctg
     5881 atatttgtgt caggatagag tatcacgatc cggtactgcc tcaggtcaag cgggaactct
     5941 gacaaaatgt tacccacccc ttcggcaaat gaaggggtac cgtctatgaa gaatggtgcg
     6001 tcgctgccga taaggcctgc aaaggagcgg agttcctcgg cgttaaggcc gaagccgaaa
     6061 tacctgttca gcgccttcag catgccggcg gcatcggatg atccgccgcc aaggcccgca
     6121 ccgacgggga tgctcttgtg aaggtgaatg cggatgccgg gaaaatcaaa cgactggcgc
     6181 atgatcttca ccgccttcat caccagattg ttgtccggat cagcctcctt catcaacccg
     6241 gttaccctca gtgagtcatt cctgcgatta tccttcctga ccacaaactc aagagcatca
     6301 gcaagatcaa cagggtagaa gaaggtctcg atgttatgat agccgtcagg cctcttttcc
     6361 acgatcctta accctatgtt tatcttcgct tttggaaata caaccatagc cggtgtctct
     6421 tgagagatca aaaatatacg aaccttttta atttacctca tctttatcga ttatatcctt
     6481 tttcaacatc ttataaacaa cttgcggtgg aaagaattag acaggctgtt tccccggaga
     6541 cggttgttat tgttaaattt gattccggct gcagtgaatg ctgcattcgt ggaactaaca
     6601 tataatcaat catatattaa tggcacaggg tacatctaaa agcaggcagg acaaaaagca
     6661 gtcggtggtc ataattccgg agggagggag gatcccgccg caggcggctg agatggaaga
     6721 ggctgtcctg ggggctgcca tgcttgagat agaggctgcc aggatggtag ttgacatgct
     6781 ccggccggag gttttttatt cggctgccca ccagaagatt tttgcgtcaa ttatcgagct
     6841 aaacaagcgc gaccagccaa ttgacctcct gacggtaacg caggagctga agaggacagg
     6901 tgagcttgag gaagccggag gtgcggttta tctcacggag ctgacctcca ggatagtatc
     6961 tgccgccaat attgaatacc atgcaaggat cgttctgcag aaatatatac agcgtgagct
     7021 gatacgtgcg gccacgatga ttcagaacct ggtctatgag gatacccttg acgtcactga
     7081 gttgcttgac aggagcgagg atgaactctt ccgcattgcc gaggggaaca tcaagcatga
     7141 ggttgcacca atacacgaag tgatcaagca gacccttgcg gagattgagg agctgggcaa
     7201 aaaggaggat gctcttgtgg gcgttccttc agggttcacg aaacttgaca ggctgacttc
     7261 gggatggcag aggtcggaac tggtgataat cgccgcgagg ccctcgatgg gaaagactgc
     7321 ctttgctctc actatggccc gtaacatggc aatagatcac ggaaggaagg ttgccatctt
     7381 ctccctggag atgtcagcaa ttcagcttgt gaacaggctt attgtctctg agaccgagat
     7441 accaggggag aagatcaaga acggcaagct ctcgccggag gagtggtctt tgcttgagtc
     7501 gcgcattaag tctcttaccg aggcgcccat ttttattgat gacaccccgg ccatctccgt
     7561 ttttgagctc agggccaagt gccgcaggct gaaggcccag gggaagcttg atgttgtagt
     7621 ggttgactat ctgcagctta tgaccgggcc gcccgatgcg ggcagccgtg aacaggaggt
     7681 gagctccatc tccaggtcgc tgaagagcat tgccaaggag cttgctgtgc cggtgctggc
     7741 gctctcgcag cttaaccggt cagtagagac gcgcggaggc aacaagaaac cgatgctgtc
     7801 agaccttcgt gaatcgggcg ccattgagca ggatgccgat atggtggtgt tcatccaccg
     7861 tcctgagaag atgggtgtcc tccagtttga agacggacag tcaaccaagg ggcatgctga
     7921 tatcatactg gcaaagaacc gtaacggccc caccgatgag gttctgttgt atttcaagga
     7981 agaccgtgcc tcctttgttg agcctgatga tttcgggcct gatgttgctt tccccgacat
     8041 tccctccgga ggcgtcttca caatggggtc aaagatgaat gatgacatat catcggacta
     8101 cagtgatttt tctcctgatg acaatggccg gagacccttt tgaattgcga gttgcgaatt
     8161 gcgatttgcg aagtggaaat tgcgtcccga tccgtcagcc ggcggatgcg ggattgatcc
     8221 tgctcctggg tcgcaggctc aaaattgcga attggtgatt tgaggaacct attctaacct
     8281 aaggataacc ctgattccgg ctgattcgaa tgatgtgccg gtgaatccgg catatacgcc
     8341 aaccgtaccg acaagtaaca cttcgctgag ggagtagcct gcttcatagt aatttccggc
     8401 ttccggcgtc cacaacgctg atatccctat attctccctg atgagtgtgc ggctgagccc
     8461 gggcaggcgc ttgagcagca gggtgggagt ggtgtatctt aaatgtgcct cggcaaatgc
     8521 tttccttgtc gagacggaaa aacatggctt cagataaaat gcatcttcat agaaattaag
     8581 cagtaccggt gacgactgtg tattgaagaa atgcatatcc tgcatcctgg cattttcact
     8641 gttgaggaaa ccaccggccc ggatccgcca gcggaactca ttcattgccc cgaatctgtc
     8701 tgtgcggttt acctccgctg caagaaggtc gtagctgcct gaaagggtgt cattatagcc
     8761 gtaaccgtgt ctccatgtca gggtaaaggt cggataatca gatcccgcac tgaccctggc
     8821 gccgccagat atgcgatacc tctgccgcgg ggtccatgtc agggatgact caagggagag
     8881 atgcctgtgg ctgaccggtg gctcatagtc gtatccttcg gctgctgaag catacgggtt
     8941 gtcaggtatg ttctgagacc agggtttttc cctcctgaag aaggagaagg aggaattatt
     9001 tggcagggga tcacgctttt cgtacttccc ctgtatgtca agtgtcaatc cgttgacaac
     9061 gtcgatacgg tgtccgccaa tgatgtattg gcttccgtaa agtctcatga tgttctccct
     9121 gaagaaaaga gaagatacgg tattaaccag ctggttaacg cctgatgcag agaattcgtc
     9181 ggactggctg cctgccctga tgtaaatatt ccctgcgtgc atcgggtcat atagcatgtt
     9241 ggcagtgaca ttccacagga gtttttccct gctgaaggca taccttgccg aaggggcaat
     9301 agtaaagcgg cctgttttgc ctgacctggc cgagagactc attcccgttc ctgccacaaa
     9361 cccgtcaacg gtgttgtagg agaagctctt caggtctgcc agtccgtcaa attcaaggaa
     9421 ggtgtttttt gacagctgcc atctctttcc gcctgtcagg tccctgatga accgggatac
     9481 agggctcgtt ttcatccctg cggagtcgga gacggcggct gagtctgatc ccggctgtga
     9541 tcttccgccg atggtgatgg tcagtgttga ggtgtcacgc actgccagtt ttccttttgc
     9601 ggccggggct gttgccaggc tggcgctctc ctcgcgggtg agcgggatgg gccgcacctc
     9661 cgcccaatac gccgaatccc ggccggtggc gccatcctcg acaatcatcg tggttttgtc
     9721 ttctatctcc aggggaggct tcccgcgcgt ggtgcggaca tttttctcat tcagccgcgc
     9781 cagccgtgac atgtcccttg ccgtcaggtc atcctttgcc agcagagcct caatctcccg
     9841 cccggcagat gttttcggcc gttcagcagg tccttcctca gccccggccg taatggcagt
     9901 ttcatatgcg gctggccgcc ggagaatcct gtcaggctct acatcaaggt atctgactgc
     9961 gctggtgtat gcagccctgg cctttactcc catgattgaa atgtcagcgt taaactcatg
    10021 gctgacaggc atccatatgc cgtcttccac tggtgtgtaa acctgcctta cccttatgcg
    10081 tccggccatg ttgtcattgg taaggtcgag ggagtgaatg gcccacaggt cctccacgat
    10141 gtatattacc ccggtgaaga gctgctggct tttgcggcgc ggggtgacgc tgatcttgtc
    10201 aatcacattg tcgccctgcg atgatgaccc aaggaatgtg aagttataat gagaaaatgc
    10261 atttggcgcc aggggtgaaa ttgccatctc caccagtaca ggctgataga aactggcttc
    10321 gatgaaatcc attggcgagg cctgcccctg agtcaggtct tcgcttgagt tggcagagat
    10381 aacccggtgt tcgtatttat ccggagcggt gaaagttatc atgttgaccg actcggtgaa
    10441 gtagtatttg cctaccttga ggtcatcacc gttcgagtcg atcttcatcc tccgcttgag
    10501 catttcagga atccggtcaa cactgccacc gcccctgatg taaacctcgg ctttccacat
    10561 cttaacctgg ttgaggtgat aaggtgccat gccgatagcc ttgcgcatta tgaaatatgc
    10621 agggtctttt cctgatgccg agaccctcac cggtggtatt tcgaagagct gttcagtcag
    10681 ggttatgtct gcagcgacgc cttcgccagt gacggcaatg tttcttgtca caggaatgta
    10741 tccgaggaac tggtagttta cgttgtagtt gccgggaggg agggagatct cgtaccagcc
    10801 ctcgttgttg gaggttgtac cctggcgcag ttcagcgata tagacagatg cggaaggcat
    10861 tggtgttccg ctgctgtcgg ttatcctgcc gcgtaatacc tgggcattga ccgaaatggc
    10921 agggaggaga agaaaggcaa caagaatcag tatccttgta cccggaacca ggccattatt
    10981 cgtaatgtac ggcaatgaag aaagtgcctc cggaatttgt ctcctgccgc
//
LOCUS       DAOPXU010000090        11003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_6838_length_11003_cov_12.306997, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000090 DAOPXU010000000
VERSION     DAOPXU010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11003
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6838_length_11003_cov_12.306997"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..1198)
                     /locus_tag="PKK03_09170"
     CDS             complement(185..1198)
                     /locus_tag="PKK03_09170"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HNX84611.1"
                     /translation="MNNLKIAFFDAKPYDTESFNEVNRHFGFTIKYFKFHLTPDNVVL
                     AQGYDAVVIFVNDTVDAAMISQLREYGVKLIALRSAGFNNVDLKAALGNIRVARVPAY
                     SPHAIAEHTVALMLTLNRKVHRAYFRTRDTNFALNGLLGFDMNGKTAGVIGTGRIGRV
                     LVKILRGFGMEVLLTDSYPDTAFAADTGSSYVSLEELYRRSDIISLNCPLTRETEFMI
                     NSSSIAMMKKGVMLINTGRGKLIRTADLIEGLKSGQVGSAGLDVYEEESQFFFEDLSD
                     RVLADDVLARLLTFNNVIITSHQGFFTREAIHNIAQTTLQNIDDFFAGRKLENEVCFN
                     CGN"
     gene            1380..2444
                     /locus_tag="PKK03_09175"
     CDS             1380..2444
                     /locus_tag="PKK03_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNX84612.1"
                     /translation="MKTRFLLILFVLTALLTAPVLMAQEAKPWYFGDPASRPEPDPKA
                     KTLPLIQVRGNKFVTPDGQTVLFRGLSISDPDKIERQGHWNKTHFEQVKKTGAMIVRI
                     PVHPVAWRERTPEKYVELLREAVDWCTDLGMYVIIDWHSIGNLWMELFQDPMYNTTKT
                     ETYEFWRTIARNFAGHNTVAFYELFNEPTIYRGELGSLPWSEWKKINEHMISLIRAYD
                     PETIPLVAGLDWAYDLSPLRDDPVNAEGIAYVTHPYAFKRGQPWEPRWEENFAFAAAY
                     VPVVATEFGMHTDMNAPDYNDYGNRIIKFLEERGISWMCWIYDPQWWPQMLKSWDYDL
                     TEGGLFFSRAMKGELPFQKK"
     gene            complement(2552..>2920)
                     /locus_tag="PKK03_09180"
     CDS             complement(2552..>2920)
                     /locus_tag="PKK03_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84613.1"
                     /translation="ILFPFNYATWNAGNFGPQVMARAKEKNMGILALKAMARGPWPEG
                     ADRSAYPKCWYEPLISEEDITMGLRFTLSHPVTAAIPPGDEGLFRQALTLGHNLKPLD
                     QSEVISIREKALKGVPLFRS"
     assembly_gap    2921..3020
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3021..3358)
                     /locus_tag="PKK03_09185"
     CDS             complement(<3021..3358)
                     /locus_tag="PKK03_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNX84614.1"
                     /translation="MKRRKFVISAASAIPAITLFPADLSGMTRETARGKIEKRSLGRT
                     GEMLSFIGFGGIVVMNATPDDAFARVRMAIDAGVNYFDVAPSYGDAEIKLGPALEPFR
                     KEVFLACKTT"
     gene            complement(3360..4283)
                     /locus_tag="PKK03_09190"
     CDS             complement(3360..4283)
                     /locus_tag="PKK03_09190"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNX84615.1"
                     /translation="MKRTIAIFTLIFLVAALAGQQQERPANLSGKPQAEIAGIPCQME
                     ELTSPQFAEAVELSGGVCVIPIGIIEKHATHLPLGTDLFEARASVIRAAEKEYAVVFP
                     PYYVGQIFEAMHQPGTIAYSNDLMWQMLEETCRELSRNGLKKIVLFNGHGGSTYFLNY
                     FCQSQLAARKDYIVVLFQGGPDEAMDRQISSLKKSKLDGHAGEEETSMMYLIRPDLVD
                     TEAMGKESGLNQGRLNAMPHAFAGIHWYARYPNHFAGDVLQTNRELGELLITRDAVQL
                     AELIRYLKNNSTIEELHKEFYNRAEYPLKNN"
     gene            4453..5367
                     /locus_tag="PKK03_09195"
     CDS             4453..5367
                     /locus_tag="PKK03_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84616.1"
                     /translation="MKKLNLLALILIMGIAFVSCDKTDATEFDVMAKSSLSEDPSGPA
                     TPAGVAPFIIPGANNGGNRTCAEVGMFFKNDPAYFDYCGEKVDFGNFKSAFPEGLMVT
                     SDGKTLSFNATDCIKIGDKYYKVGAVIVKGGNSANVYFYEGGTLGDVNLTAPANASGK
                     PAGISNVTFCYIECDEQPELVIAFKSYLNNSDWVCTTGGPGNIGFVAYYDFKPGVVGK
                     VFLSANTNPSTGDLTKPVGDITVADIDGDGLWEVTIDNVDRPELLYKNAYLFVGTLAE
                     YTGKYYLNFPFQTGEITPVAPLTFQLSF"
     gene            complement(5567..5642)
                     /locus_tag="PKK03_09200"
     tRNA            complement(5567..5642)
                     /locus_tag="PKK03_09200"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Lys,seq:ctt)
     gene            complement(5733..>6543)
                     /locus_tag="PKK03_09205"
     CDS             complement(5733..>6543)
                     /locus_tag="PKK03_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84617.1"
                     /translation="SLHLLTRTEAMNRERKIWLIIAASLLAIFIIYYLVMSVSAVSRT
                     VNDFDNAYFASLEQKEGDTINLCAIPGYIDMIRDKAFLSSQVKLAESDSVGLLINVRD
                     SVIQLLIKGLPVRTVEIDEYDVSPFFHRANQEAIYSMLSAPLNITGMQATFMKNPVSV
                     KIAPRDTSQAVESVKPDTTDFEAVFYTLDTDRNIRFFFEQQEDTIGADRRARFFFDLK
                     DRMRNASVTMKAVTRLKTPPYVPYIKIWIPKAEAKIIYRAIPREGMIVLTQ"
     assembly_gap    6544..6643
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6644..7716)
                     /locus_tag="PKK03_09210"
     CDS             complement(<6644..7716)
                     /locus_tag="PKK03_09210"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNX84618.1"
                     /translation="MKRGSLTNIIVYAIVGIVLTIIGVLVFRALVPEPPLPEVEQARA
                     AIARARDLQSEIYSPKLFREAKNNYDSAMAVWRSENDRFILARDYERVIQFAEIAQKK
                     AEDASRNTISRSKSLKSNLESEITRLNHEMATFEKIFLSMPLPQDVKKKHARGKLLIK
                     EAGIDFKKERYVDGNVKITEANEYISGTYNLARKNLEEYFKHYDNWQDWADETITESK
                     RSGSYAILVEKIPAQLHLYQGGKKKYTFEAEFGSNWLGDKKSRGDMATPEGKYTVTKK
                     LSGGSTKYHKALLINYPNKIDVQEFNERIRNGQLPADASIGDLIEIHGDGGKGGNWTQ
                     GCVALKNSDMDMLFKYVSKGTPVT"
     gene            complement(7783..8382)
                     /locus_tag="PKK03_09215"
     CDS             complement(7783..8382)
                     /locus_tag="PKK03_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84619.1"
                     /translation="MKNKLFLAIATVAVAALMASCAKVPQEALDAAKAALETAKTAQT
                     DVYFPVEFAGLNDSLTVMLQNIETQKSKSAKDFKAIKVAAEGIALKAAELSGNVEAKK
                     AEVKAEAETMMAGAKTLLDEAKALLLKAPKGKEGKAVVEEMKNELTVLETSLTDTQTL
                     FDGGSNYMEVVDKVKAATEGINGIIAELKAAMEKAGIKI"
     gene            complement(8948..10168)
                     /locus_tag="PKK03_09220"
     CDS             complement(8948..10168)
                     /locus_tag="PKK03_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNX84620.1"
                     /translation="MNDLAGNKRIESLDVLRGLALILIIFFHSSIYNFANIHKIDFED
                     PPLLIIIMSFMALWGGIFIIYSMVVNTIMLSRRSEGSLIFKPFLYVVIAGLIYVIIHY
                     LLNIFLGRWNIDFINNMPDLTVVAASLRDSKLEFPGYTKYFEGSSLSTIAFNLILLTP
                     VLFLLLKHIRKERTQYLLLGLSIALIMILSFARISIFPSFERAVESRHFLAATFFSFT
                     IANPYPLLPYSAYGLTGMMIGLMAYHGRRRLLKLYALPLGTLFCVYGLAGMTEFDKSI
                     SRPDFFWYFKTNFELGFFILLIVTALLTQAKNNRLLTRMALLKWFSRVSLTIYLLETT
                     TSELFRVLWFHVVPGWDQTINGCLLFGGMNVLLWSIILFFWQKAGFLCSLEYFWVKMF
                     GRLGKQSTKMDFIR"
     gene            complement(10161..10358)
                     /locus_tag="PKK03_09225"
     CDS             complement(10161..10358)
                     /locus_tag="PKK03_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84621.1"
                     /translation="MSESEGDLRLMSNMITSEGSIIDPAALMEFAEIPDSACDPGERG
                     RIDFLSSDKLSRIKKESDNNE"
     gene            complement(10321..>11003)
                     /locus_tag="PKK03_09230"
     CDS             complement(10321..>11003)
                     /locus_tag="PKK03_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84622.1"
                     /translation="VSYAEILYPDVKKYLSDVLHCPVHEIYKCTEAPMAITCKYGNLH
                     INEDLVMVETFNNDGSETSSGQTCNRLIVTDLHKKSQPIIRYELNDIITISPEKCRCG
                     SNFRVIQSIQGRADDLFWARNTESNKWQPIFPDYISRAIISSSEEIDEYQATQDSPEH
                     VLVRIKLKDDANPEEFDRGVIAESVKDVFSKYKCNLPEVSVEFGSPESGKNSGKLVRI
                     RRRFEVNE"
BASE COUNT         2545 a         2820 c         2645 g         2793 t
ORIGIN      
        1 cggcgcctcc ctttttccat ggtggttagc cctccgaaga aaacccgtaa gcgcggcggt
       61 gcagttatcc ggactgcagg acctgacgaa gtaacagacc ctcagacctc agattgagga
      121 accgccgccg gcgggaccga aatcccgatg cccgaagggt gtcgggacct tcagacttcc
      181 gacctcagtt cccgcaattg aaacagacct cattctcaag ctttcgcccg gcaaagaagt
      241 cgtcaatgtt ttgtagcgtt gtttgtgcga tattatgtat ggcctcgcgg gtgaaaaacc
      301 cctggtgcga tgtgattatc acattgttga atgtaagcag acgcgccagt acgtcatcag
      361 ccagcaccct gtcagagaga tcctcgaaga agaactggct ctcctcctcg taaacatcga
      421 gccccgccga cccgacctgt ccgctcttga gcccttcaat caggtcggca gtcctgatca
      481 gcttgccacg gccggtgttg attagcatca cccccttttt catcatcgca atggatgagg
      541 agttgatcat gaattccgtt tcgcgcgtca gcggacagtt cagcgagatg atgtccgacc
      601 tcctgtaaag ctcctcaagg ctcacgtaac tgcttccggt gtcggcggca aacgctgtgt
      661 ccggatagct gtcggtaagc agcacctcca tcccgaatcc ccgcaggatc ttcacaagca
      721 ccctgcctat gcggcctgtc ccgatcaccc cggccgtctt gccgttcata tcgaatccca
      781 gcagcccgtt aagggcgaaa ttggtgtcgc gggtccggaa ataagcccga tgcaccttcc
      841 ggttcagcgt cagcatcagc gccacagtgt gctcggcgat ggcgtgtggc gagtaagcag
      901 gcaccctggc caccctgata ttgccgaggg ctgccttgag atcgacgttg ttgaatcccg
      961 cagagcgaag ggcaatcagc ttcacaccgt attccctgag ctgactgatc atggctgcat
     1021 cgactgtgtc attcacgaag atgacgacgg catcatatcc ctgtgcgagc actacgttgt
     1081 caggcgtcag gtgaaacttg aaatacttga ttgtgaaacc gaaatggcgg ttcacctcgt
     1141 tgaacgattc ggtatcataa ggtttggcgt cgaaaaacgc gatcttcaga ttgttcattc
     1201 cttactggtt gttaaatgat aacaagaggc gcctgcattt gttcttaaac aggtgcagtt
     1261 acagacggag ggtgattttg acatatccgg gttggcaata aatcaaacgg atttactaaa
     1321 ttcataacct tcaatttgtt gaaaccgcat tattgcagta acctcataac tgtcacgata
     1381 tgaaaacaag attcctcctt attcttttcg tgctgaccgc actgctcaca gcgccggtcc
     1441 tcatggctca ggaagccaaa ccctggtact tcggcgaccc tgcctcgcgg ccggagcccg
     1501 atccgaaagc aaagaccctg cctctcatac aggtccgggg gaacaaattc gtcaccccgg
     1561 acggtcagac agttctattc cgcggactct caatctctga tccggacaag atcgagcggc
     1621 agggccactg gaacaagaca cacttcgagc aggtgaaaaa gaccggcgca atgatagtgc
     1681 gcattcccgt tcacccggtc gcatggcgcg agcgcacacc cgaaaagtat gtggaactgc
     1741 tgcgtgaagc ggttgactgg tgcaccgacc tgggcatgta tgtgataatc gactggcatt
     1801 caataggcaa cctctggatg gaactcttcc aggatccgat gtacaacacc acgaaaacag
     1861 agacatatga gttctggaga actattgcac gcaacttcgc cgggcataat acagtggcct
     1921 tttacgagct cttcaatgaa ccaacaatct accgcggcga gctgggctca ttgccctggt
     1981 ctgagtggaa aaagatcaat gagcacatga tcagcctgat aagggcatat gatccggaaa
     2041 ctattccgct ggttgcgggg ctcgactggg catatgacct ctctcccctg cgtgatgacc
     2101 cggtgaatgc ggaagggatc gcctatgtga ctcaccctta cgccttcaag cgcggacagc
     2161 cctgggagcc gagatgggaa gagaacttcg cgtttgctgc ggcttacgtg cctgtggttg
     2221 ccacggagtt cgggatgcac actgacatga atgcccctga ttacaatgac tacgggaatc
     2281 gtatcataaa gttcctggag gagcggggca tcagctggat gtgctggatc tatgatccgc
     2341 agtggtggcc gcagatgctc aaatcatggg actatgacct gactgaaggg ggtctcttct
     2401 tcagcagggc catgaagggg gaactgccgt tccagaagaa gtagagtacc ggacttccgg
     2461 ggccgggtgg atactcctcc ggaattcagt cattcactat gaggccggaa agcttccctg
     2521 gctgattgaa gtgatattcc gcctgaaagg ttcaggacct gaacagggga actcccttca
     2581 gcgctttctc cctgatgctg ataacctccg actgatcgag gggcttcagg ttgtgcccca
     2641 gcgtgagagc ctgcctgaac agcccctcgt ctcccggagg tatggctgct gtgaccggat
     2701 gagacagggt aaacctcaat cccatcgtta tatcttcctc actgataagc ggttcgtacc
     2761 agcattttgg gtaggctgac cggtcggccc cttcaggcca cggtcctctc gccatcgcct
     2821 tcagtgccag gatgcccata ttcttctcct ttgccctggc cataacctgc ggcccgaagt
     2881 taccggcatt ccaggtagca taattgaacg ggaaaaggat nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn tcagtggtct tgcaggcaag aaaaacctct ttccggaacg
     3061 gttcaagtgc cggacccagc ttgatctcag catcgccata tgacggggca acgtcgaaat
     3121 agtttacccc ggcatcgatc gccatcctta cccttgcgaa tgcatcgtca ggagttgcat
     3181 tcattacgac gattcctcca aatccgatga acgaaagcat ctcgcctgtc ctgccgagag
     3241 atctcttctc tatcttgccc cgggctgtct ccctggtcat cccggagaga tcggctggga
     3301 aaagcgtgat ggcaggaatc gccgatgctg ctgaaataac gaattttctt cttttcatct
     3361 cagttatttt tcagagggta ttcagccctg ttgtaaaact ctttatgaag ctcttcaata
     3421 gtgctgttgt tcttcaggta ccggataagc tccgccagct gaaccgcatc cctggttatc
     3481 aggagctctc ccagctcgcg gtttgtctgg agcacatcac ctgcgaaatg gttgggatac
     3541 ctggcatacc agtggatccc tgcaaatgcg tgaggcatgg cgttaagcct accctggttc
     3601 agccccgact ctttgcccat agcttccgtg tcaaccaggt caggacggat caagtacatc
     3661 atagaggtct cttcctctcc tgcatgaccg tcaagcttag acttcttcag gctgctgatc
     3721 tgccggtcca tagcctcgtc gggcccgccc tggaagagca caacgatgta atctttcctc
     3781 gcggcgagct gcgactggca gaagtagttc aggaagtaag tgcttccgcc gtggccgttg
     3841 aaaaggacaa tctttttcag cccgttacgc gagagttccc ggcaggtctc ctcaagcatc
     3901 tgccacatca ggtcgttgct gtaggcgatg gtccctggct ggtgcattgc ctcaaatatc
     3961 tgccccacat agtatggcgg gaaaacaacg gcgtactcct tttctgccgc ccttatgact
     4021 gaggccctcg cctcaaagag gtctgtcccc aggggaagat gggttgcatg tttttctatt
     4081 atccctatcg ggatgacgca gacgccgccg gacagctcca cagcttcagc aaactgcgga
     4141 gaagtaagtt cttccatctg gcaagggatg ccggcaatct ccgcctgcgg tttcccggag
     4201 agatttgccg gtctctcctg ctgctgtccg gccagtgcag ctaccaaaaa aatcagtgta
     4261 aaaatcgcaa tagtcctctt catcgctctg cagtatttaa atcatttaaa attagatgtt
     4321 ccggccgact aatcaaaaac tattcgcacc gccattcgtc aaaaaatcag gtttcttcat
     4381 catatcttca caaccatatg cttattgttg tgttatgctg acagtgaata ttaaaccaaa
     4441 cactttatgc gtatgaaaaa actgaacctt ttggcactta tccttatcat gggaatcgca
     4501 ttcgtttcct gtgacaagac cgatgcgacc gaatttgacg tgatggcgaa atcatcactg
     4561 tctgaagatc caagtggtcc ggccacaccg gctggtgttg ctccgtttat tattcccgga
     4621 gccaacaacg gagggaacag gacctgtgca gaagtgggaa tgttcttcaa gaatgacccg
     4681 gcctactttg actactgcgg agaaaaagtg gactttggca atttcaaaag tgcgttcccg
     4741 gaagggctta tggtcacttc agacgggaag actctctcct ttaacgcaac tgattgcatt
     4801 aagatcggcg acaaatacta caaggttggc gcagttatcg tgaaaggcgg aaattcagcc
     4861 aacgtttatt tttatgaggg tggcaccctg ggtgatgtca acctgaccgc gcctgccaat
     4921 gcgagcggca agccggcagg aataagcaac gtgacgttct gctacataga gtgcgatgag
     4981 cagcctgagc ttgtgatagc attcaagagc tacctcaaca acagcgactg ggtttgcaca
     5041 acaggcggac cggggaacat cggcttcgtg gcttactatg acttcaagcc gggcgtcgtc
     5101 gggaaggtgt tcctctcagc caataccaac ccctcaacgg gtgaccttac caaaccggta
     5161 ggtgacataa ctgtggcaga cattgacggt gacggcctgt gggaagtcac aatcgacaac
     5221 gtggaccgtc ctgaactgct ctacaagaat gcatatcttt ttgttggaac acttgctgaa
     5281 tacaccggta agtattatct gaatttcccc ttccagacag gtgagataac acctgtagca
     5341 ccgctgactt tccagctgtc tttctagagg atttttcagt ctacaatagg aatttttgat
     5401 ggcagaggct gccccggcta acccgggcag cctctttctt tcctggtcag tctgcaggga
     5461 ctcgaacccc ggaccctccc gatccgccgg ctggcggatc cgggatgctc tgccatctga
     5521 gccaaacaaa aaaaggagcc tgtgcggctc ccttctgatt tattcctggt cggtctgcag
     5581 ggactcgaac cctggacccg ctgattaaga gtcagctgct ctaccatctg agctacagac
     5641 cggaattaaa acggtgcaaa agtacacttt tttgggaatt gtgcattaaa aatttcgcat
     5701 caaaaatgtc agctcctgag gcgtatgata cgctattgcg tcagaactat catcccctcc
     5761 cgcggaatgg cccggtaaat gatcttcgcc tcagccttgg gaatccagat cttgatatac
     5821 ggcacatacg gcggggtctt cagcctcgtg acagccttca tcgtcacgga agcattcctc
     5881 atcctgtcct tcagatcgaa aaagaacctc gcccgccggt cagcaccaat ggtatcttcc
     5941 tgctgctcaa agaagaaccg gatattacgg tcagtatcca gcgtgtaaaa taccgcctcg
     6001 aaatcagtgg tgtcaggctt tacactctcc accgcctgcg acgtatccct gggagcaatc
     6061 ttcacactaa ccggattctt catgaaggtg gcctgcatac ctgttatgtt cagcggcgca
     6121 gagagcatac tgtaaatggc ctcctggttt gcacgatgaa aaaacggcga gacatcatac
     6181 tcgtcaatct ccaccgttcg caccggcaat cccttgatca gcagctgaat cacgctgtcg
     6241 cgaacattga tcagcaaacc caccgagtcc gactccgcca gcttcacctg agaactcagg
     6301 aaggccttgt cacgtatcat atcgatatat cccgggatgg cacacaggtt tatcgtgtct
     6361 ccctccttct gctccagcga agcaaaatac gcattgtcaa aatcattcac ggtacggctg
     6421 acagccgaaa ccgacatcac cagataatag atgatgaaaa ttgccagcag agaagcagca
     6481 attatcagcc agatttttct ttcccggttc atcgcttcag ttcttgtcag taaatgtaaa
     6541 ctcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtcaccg gtgttccttt
     6661 tgaaacatat ttgaataaca tgtccatgtc agagtttttc agggcgacgc atccctgtgt
     6721 ccagttgcct cccttgccgc cgtcgccgtg aatctcaatc aggtcgccga tgctggcgtc
     6781 ggccgggagc tgtccgttcc ttatcctctc gttgaattcc tgcacgtcaa tcttgttcgg
     6841 atagttgatt aacagggctt tatgatattt agtcgagcct ccggaaagct tcttcgtaac
     6901 agtgtatttt ccctccggcg tagccatgtc tcccctgctt ttcttgtcac ccagccagtt
     6961 gcttccgaat tctgcctcaa aggtatactt ctttttgcct ccctggtaca ggtgaagctg
     7021 cgccggaatc ttttccacga gaattgcgta tgatccgctc cttttgctct cggtaattgt
     7081 ctcatcggcc cagtcctgcc agttgtcgta gtgtttgaag tattcctcga gatttttcct
     7141 ggctaaattg taagtacctg atatatactc gtttgcttcg gtgatcttga cgtttccgtc
     7201 gacgtagcgc tcttttttaa agtcgattcc cgcctccttg atgagaagtt ttccgcgggc
     7261 atgtttcttt ttgacatcct gagggagcgg catcgagagg aagatctttt cgaacgttgc
     7321 catctcgtgg ttaaggcggg tgatttcgct ctcgaggttg cttttgaggc ttttcgacct
     7381 tgaaatggtg ttacgtgagg cgtcttcggc cttcttctga gctatctccg cgaactgtat
     7441 gacccgctcg taatcgcgtg ccagtatgaa acggtcgttt tcgcttcgcc atacggccat
     7501 agctgagtca tagttatttt tcgcctcgcg gaaaagttta ggtgaataga tctccgactg
     7561 cagatccctg gccctggcaa ttgcggcgcg ggcctgttcc acctcgggca gcggcggctc
     7621 gggcacaagg gccctgaaaa ccagcactcc gatgatggta agcacgatgc cgacgatagc
     7681 gtaaactata atgtttgtca gggatcctct cttcatgggc gcgaagttaa aaaaatcccc
     7741 gggaccaggc ccggggatcc ttttattagt taaacggtac ggttagattt tgataccggc
     7801 tttttccatt gctgccttca gctcggcgat gatgccgttg atgccttcgg tagctgcttt
     7861 tactttgtca acaacttcca tgtagttgct gccgccgtcg aagagagtct gagtgtcggt
     7921 gagagaggtc tcgaggacgg tcagttcgtt cttcatttct tctacaactg ctttgccttc
     7981 cttgcctttg ggagccttga gaaggagagc cttagcctcg tcgaggaggg ttttggcgcc
     8041 tgccatcata gtttcggctt cagccttaac ttcagctttc ttggcttcta cattgcctga
     8101 cagttcagct gctttgagag cgataccttc agcggcaacc ttgatagctt tgaaatcctt
     8161 tgctgacttc gacttctgtg tttcgatgtt ctggagcatg acggtaagtg agtcattcag
     8221 gccagcgaat tctaccggga agtaaacatc agtctgagct gtttttgctg tttcgagagc
     8281 agcttttgca gcgtcaagag cttcctgggg aactttagcg cagcttgcca tcagggcagc
     8341 tacagcaacg gtagcgattg ctaagaaaag tttgtttttc atttctgttt aaaatttggt
     8401 tctgttataa tgtttaaaat gtctcaggtt ttgtatcact aaaccgatgc aaatttatat
     8461 aataatttta atccggtttt aatctccggc agtttttttt aatggaaatt taatctcggc
     8521 ttggcggatt tcccgggatg gccctggctg ggggtttccg cctgcagggc gaagctcttg
     8581 ctctgttggt taaggggtgt tgaaggggac ttttatggta taagggggct ggtggcagga
     8641 cgaaattgat gttttacggc agggattgag gctaccgata ttcacattct ctttggcgga
     8701 gctgtattgc cggcaccccg gccgctcact ctccgcacct tcgtaactgc acagaaagct
     8761 tatgtgcccg atagctctta tcagaattta gaatcaaaag gccctgtgtc cttctcaggc
     8821 atacacgatt tgcagggtct gccccgtcat gcatgatttg aagggtctgc cccgtcatgc
     8881 atgatttgaa ggacattctc cgggtcacac gatttgcgga attttccggc ctgtaccaat
     8941 aaaaaatcta tcggatgaaa tccatctttg tcgattgctt cccgagcctc ccgaacatct
     9001 tcacccagaa atattcgagg ctgcacagga atcctgcttt ttgccagaag aataatatta
     9061 tactccacaa caacacattc atcccgccaa agaggaggca cccgttgatg gtctgatccc
     9121 aaccggggac aacgtgaaac cagagcaccc tgaacagctc cgatgttgtt gtttccagaa
     9181 ggtatatcgt caggctgacc ctgctgaacc acttcaggag tgccatgcgg gtcagcagcc
     9241 gattattctt cgcctgcgtc agcagtgcgg ttacaattag caaaatgaag aaccccagtt
     9301 caaaatttgt cttgaaatac cagaaaaaat ctggccttga gattgacttg tcaaactctg
     9361 tcattccggc cagtccatag acacagaaca gcgtacccag tggcaacgca taaagtttca
     9421 gcaacctcct cctgccatga tatgccatga gcccgatcat catgccggta aggccatagg
     9481 ccgaataagg cagaagcgga tatggattgg ctatggtaaa actgaaaaag gttgctgcca
     9541 gaaagtgacg cgattcaaca gcccgttcaa acgacggaaa aatcgagatc ctggcaaagg
     9601 agaggatcat tataagcgct atggagagac ccaggagcag gtattgggtt ctttccttcc
     9661 tgatatgttt caacagcagg aataagaccg gggtaagcag aatcaggttg aaggcgatgg
     9721 tggacagcga tgatccctcg aaatatttgg tataaccagg gaactccagc ttcgaatccc
     9781 tcagtgaagc ggccacaacc gtcagatcag gcatgttgtt gatgaaatcg atattccacc
     9841 ggccaaggaa aatgtttagc agataatgga tgattacata gatcagcccg gcaatcacaa
     9901 catacaggaa aggtttgaat atcagactcc cttcgctcct tcgcgacaac ataattgtat
     9961 taacaaccat ggagtaaatg atgaaaattc ctccccagag agccatgaaa ctcattataa
    10021 taataagtag cggagggtct tcaaaatcga ttttatggat attggcaaaa ttatagatcg
    10081 atgaatgaaa gaaaatgatc agtatcagcg cgagtcccct cagcacatcg agcgactcga
    10141 tccttttatt tcccgccagg tcattcattg ttgtctgatt ccttttttat tctggaaagt
    10201 ttatctgagc tgaggaagtc aatccttccc ctctcaccag gatcacaggc cgaatccggg
    10261 atttctgcga attccatcaa tgctgccgga tcaatgattg aaccttctga tgtaatcata
    10321 ttactcatta acctcaaatc tccttctgat tctgacaagc tttcctgaat ttttgccgga
    10381 ttccgggctt ccgaattcaa ccgatacctc aggcagattg catttgtact ttgaaaaaac
    10441 atccttaacc gactctgcta ttacgcccct gtcaaattct tcaggattcg catcatcctt
    10501 cagttttatc ctcaccagta catgttccgg ggagtcctgt gtggcctggt actcatcaat
    10561 ctcctccgac gatgatatta tcgccctgct tatataatcc gggaaaatgg gttgccattt
    10621 gttgctctct gtgttcctgg cccagaaaag atcatcggcc ctgccctgga tggattgaat
    10681 gaccctgaaa ttcgaaccgc accggcattt ttcaggactt atggtaatga tatcattaag
    10741 ctcatagcgg ataatgggtt gtgatttttt gtggagatcg gtgactatca gcctgttgca
    10801 tgtctgtccg gaactggtct ccgatccgtc gttattaaac gtctcaacca ttaccaggtc
    10861 ctcattgata tgcaggttgc cgtatttgca cgtgattgcc atcggtgcct cggtgcattt
    10921 gtagatctca tgtaccgggc agtgaagcac gtcggacagg tactttttca cgtcgggata
    10981 aaggatctcc gcataggaga cta
//
LOCUS       DAOPXU010000091        10987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_6858_length_10987_cov_12.026802, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000091 DAOPXU010000000
VERSION     DAOPXU010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10987
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6858_length_10987_cov_12.026802"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PKK03_09235"
     CDS             <1..1131
                     /locus_tag="PKK03_09235"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNX84623.1"
                     /translation="WSSYTVPGYFSATPLKGINGAVWFRKEINIPTSAAGQPGTVNLG
                     RIVDADSAFLNGTFIGTISYQYPPRWYTFPAGILKEGKNVLTVRLISNIGDAGFVPDK
                     SYELTAGDFKASLEGEWKYKVGAALPPLRGQTFFGYKPAGLYNAMLAPLLNCSIKGIL
                     WYQGESNAERPEEYRTLLPAMIRDWRNSLGQGDVPFLVVQLPNFMESRETPYDSGWAL
                     FREAQTEALKLPATGMAVTYDIGEWNDIHPLNKRDVGVRLALAARKVAYGDSEVVASG
                     PTYKSMRVRGRRIIISFDNTGSGLTVRNGKRPGHFAIAGEDMQFVWAKARVRGNRVVV
                     KSREIKKPVAVRYAWADNPVGANLYNEEGLPAMPFRTDQVKM"
     gene            complement(1190..1873)
                     /locus_tag="PKK03_09240"
     CDS             complement(1190..1873)
                     /locus_tag="PKK03_09240"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNX84624.1"
                     /translation="MRLSYRYSELLTGIDALAGVMGYLPGTAPEPVTDLIAVLSEELL
                     ATGEARAEYIIFDHVNLDPVSKTVEFEGVVFHVKPIIFSQIKNAEGVALFICTAGREV
                     GELSRKSMKEGDLLRGYVYDVIGSEVAEGAADRMQEALKTAVALSGMGITNRFSPGYC
                     GWDVAEQHKLFSFFTENYCGITLTDSALMNPVKSVSGMIGIGRKVKYAPYKCQLCDDK
                     NCIYRGRKT"
     gene            complement(1870..2718)
                     /locus_tag="PKK03_09245"
     CDS             complement(1870..2718)
                     /locus_tag="PKK03_09245"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNX84625.1"
                     /translation="MKTILKGTLKEVVIDTEGPVVIIGECINPTRRKKLVSTLQANDF
                     SYMLELAKSQIDAMADVLDVNVGFPGVVDEELLPQAVIELQDHFDIPLCLDSPNPKAI
                     EAALKVARGKCLINSVNAEEASLQRLLPVAREYGAAIIGLVLDDDGITHDPEKRLKIA
                     EKIIERAVRSGIKEEDVIIDPMAMAVSADPNACRVTLETIRLVHDRLGHNITQGASNI
                     SFGLPHRENINNAYMAMSILYGLTCPIANPEKITGLVRAADLVLGRDDYAIRFIEHYQ
                     STQLPK"
     gene            complement(2839..3504)
                     /locus_tag="PKK03_09250"
     CDS             complement(2839..3504)
                     /locus_tag="PKK03_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HNX84626.1"
                     /translation="MTEILERISTCVEFGKVDIKSPYPPAMKDQEGAFELTRHALDEG
                     IKPGEILEEALIPAMSRVGQKFSRNEIFVPQMLMAAKAMSSSMIHIKPYYLSGETKRR
                     GTFIIGTVRGDLHDIGKNLVSMMIEGGGWEIIDLGVDVSTEKFIEAAEKHPGAVIGLS
                     ALLTTTMENMRKSVEVLRNRFPAMKILVGGAPVTEDFCRKIGADFYSPDPQGAVEYLK
                     TLA"
     gene            complement(3709..5667)
                     /locus_tag="PKK03_09255"
     CDS             complement(3709..5667)
                     /locus_tag="PKK03_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007173839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX84627.1"
                     /translation="MKRKLINSVLGTAIALLLFASCSDILEEQPRAIYTPETFKTPEG
                     VMGGLTAEYATLRRLWGDAYFYSNGLTGTDEATWAASGGTGNFKIMDLSGAGGPITPS
                     ASDAGGLWSNAFPAINTANGIIENATAVGTISEALIAEARFFRAFHYFYLVQTFGGVP
                     LDFGSGELAFNISPVRTSVRNTVPEVYTKAIFPDLLAAAEDLPATGRLTGTLTKTAAR
                     LYLSKAYLTYAWWLENPNDIPTYPDSPRTDPDGHNAAWYYQEAYNVATAAIDDPGPFG
                     LLPYFYDVNLGSNDRNKEMVLYADRTQASDYWNDANLSYASGGGADNFAAWMMTWNYT
                     TMTSSKTPWANPSTVNSVQREAEQHLGRPWTRMAPPIEVFTNTFADKTNDSRYDGTFV
                     TVYRGNWNKGGVTNAFLYNANDMMVYPGDPILTFLDSDPGGIDYSNSVYKSNIGAGVL
                     PGRADYVIAPNAISRLVHPGCGYKIGTYRTDNGVGLGQPNGSITRPFPIAKFSELYFI
                     AAEAAAKGANTVTGKTALDLINVIRARAGKWRWNNNGNAPKIEDHSADMIAATPATIT
                     VDYILDERSREYYGEGVRWWDLVRTQTWGTKALTYTICGPNLGDHTPVAVTRTIEKYH
                     YLRPIPQSQLDGLEMTAAEKAAYQNPGY"
     gene            complement(5680..8829)
                     /locus_tag="PKK03_09260"
     CDS             complement(5680..8829)
                     /locus_tag="PKK03_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX84628.1"
                     /translation="MINEPLTQGILLKKYFGKLLLSITLIAMLILVQTGMAFASGSNP
                     EELNLNQQRVVTGLITESDGNPLPGVTIMEKGTNNGSLSDANGQYRIVVTTANPVLVF
                     SFVGYTTQEVTVGSQAVVNLALAASDIGLEEIVVIGYGTARKSDVTGSVASIGGDAIR
                     DVPSSNISQALQGRVSGVDMSQVSTKPGATMQIRVRGTRSLNASNDPLVVIDGIPFAG
                     SIGDISPSAIKSIDILKDASSTAIYGSRGANGVILITTNKGYAGQQARISLDSYVGMK
                     QVFAKYPMMNGPEFVALRAAANQYTNTLDESNDMDTDWQDLLYKTGMVNSHDLGITGG
                     TQTGNYTFGVGYYKDEAVVPLQDYTRYSIRSSLDQGIGKYLRFGISTNTNYSVTNGNN
                     LGAVGMALDHSPIANIYNEDGSLKQRFMQATSGAQWVSTREVLEGLGDKYIDQNRAFG
                     TYNALYGEVKIPGIDGLKYRINLGLNYRQSNNGSYTGVGVFSGTPSNNSTASISNSHT
                     INWAAENLLTYDKTFAGKHQLNAVAMFSAEQSMYNSSNVSTKDIPIDAFQFYNLGQAT
                     ADKTVNPSNQRYELTGLLSYMGRVMYSYDSRYMISVATRTDGSSRLAPGQKWHTYPAV
                     SVGWNIANEQFMKSISQINLLKLRMGYGETSNQSVDPYKTLGLLNTRPYNFGTVYSIG
                     MYVSELPNPTLGWEFSKTFNIGLDFGLLKNRLSGTVEYYAMYTHDVILSVGLPSTSGV
                     GSYMANIGKTENKGLEFSVDGVIIDNLNGWTWEAGVNLYANRNKLVELASGQLKDETN
                     WWFVGHPIDVIYDYENIGIWQQNDPYFNILEPGGNIGMIKVKYTGDYLNDGVTPARAI
                     TAADRQIIEMEPDFMGGFNTRLAYKGVDLSIIGAFKKGGILNSVLYGSGSYLNNLNTR
                     SGNNVRIDYWTPDNTDARYPKPNGVGGDNPKYGSTMGYFDASYLKVRTITLGYYLDSN
                     EWIKRAGFKTLRVYATVQNPFVLFSPYTKESGMDPETNSYGNENAAVPLSGSLRRLLT
                     IGTNTPATRNYMIGINMSF"
     gene            9359..10864
                     /locus_tag="PKK03_09265"
     CDS             9359..10864
                     /locus_tag="PKK03_09265"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HNX84629.1"
                     /translation="MKRTQFLPICMIIFGFVSGNLFSQNPTVIKIDPDRTIATVDPNI
                     YGAFVEPIRTVVYGNIYDPGSSFADENGFRKDVIQLLRELKIPVVRWPGGNYVSGYNW
                     EDGIGPKDQRPARLDLAWHQVETNQMGTDEYVKLCKLIGSENFICINAGTGTLDQARH
                     WVEYCNIEKGTWYSDLRRKYGNETPFKVKYWALGNEIDGPWQMGQKSAEDYVKFALEA
                     AKLMQWVDKDIKLIASGASNYKPDNNWIDWNDHVLTKMAGSIDYLSVHRYATEALGND
                     RSFSGMMCLGLDLDQKIETVKALILKAMMKTGSKRPVYISFDEWSAGFGNSITASLMV
                     AQHLNSFIRHADIVKMANITMLSSLVGYSPEGADKNALFKAFYLYSNNCHGKALDVYA
                     SCEKYSNDVFKDIPYLDVTAVLNDATGTIVLNVVNRHETNPITADIVLQTGEFTGSAI
                     ANEVNGKIVVPGNPRASEPVNSTSADIKFKGNTVKYTFPAHSLTQLMLPLK"
BASE COUNT         2848 a         2631 c         2531 g         2977 t
ORIGIN      
        1 tggagcagtt atactgttcc gggatacttt tcggcaacac ctctgaaagg gatcaacggt
       61 gccgtatggt tcaggaaaga gatcaacatc cctacatctg cagccgggca gcccggaacg
      121 gtcaaccttg ggaggatagt cgatgcggat tctgcatttc tgaacggcac cttcatcggg
      181 acaatatcct accagtaccc tccgagatgg tacacgttcc cggccggcat attgaaagag
      241 ggaaaaaacg ttctgacggt gagattgatc agcaatatcg gtgatgcggg cttcgtccct
      301 gacaagagct atgaactgac ggccggtgac tttaaggcca gtctcgaagg tgagtggaaa
      361 tacaaagtag gggctgcact tccaccgctg agaggacaga cattcttcgg ttataagccg
      421 gcagggcttt ataacgctat gctggctccg ctgctgaatt gcagcatcaa gggcatcctt
      481 tggtaccagg gtgagtccaa cgcagaaaga cctgaagagt acaggaccct cctgccggca
      541 atgatacggg actggcggaa cagcctcggc cagggcgatg tgccgttcct ggtggtccag
      601 ttgccaaact tcatggaatc acgggagacc ccgtatgaca gcggctgggc gctcttcagg
      661 gaggcacaga ctgaagcgct aaaattgcca gcaacgggaa tggcggtcac atatgacata
      721 ggggaatgga atgacatcca ccctctgaac aagagggatg taggtgtcag gctggccctg
      781 gctgccagga aagtagcata cggtgattca gaggtggtag cctcaggccc cacttacaaa
      841 tcgatgaggg tcaggggccg gagaatcatc atttcgtttg acaacaccgg atcgggactg
      901 acggtacgca acggcaaaag acctggtcat tttgccatcg ctggtgagga tatgcaattc
      961 gtatgggcta aggccagggt tcgcggaaac agggtagttg ttaaaagccg ggagattaag
     1021 aagcctgtgg ccgtcaggta tgcctgggca gacaatcctg tcggagccaa tctctataat
     1081 gaggaaggac tgccggcaat gccattccgg actgatcagg tgaaaatgtg attttcgatt
     1141 tgagatttgc gatgttcgat ttaactcgca actcgcaatt cgcaaatcct caggtttttc
     1201 tcccccggta gatgcaattt ttgtcatcgc acagttgaca cttgtacggg gcatacttaa
     1261 cctttctccc aatgccgatc atcccgctga ctgatttcac cggattcatc agggcagagt
     1321 cagtgagagt gatgccgcag taattttcgg tgaagaaact gaagagttta tgctgttcag
     1381 ccacatccca tccgcagtaa ccggggctga agcggttggt aatgcccatg cctgataatg
     1441 ccacagcagt ttttaaagcc tcctgcatcc tgtccgcggc gccctccgcg acctccgatc
     1501 caattacatc gtatacataa ccgcgcagaa gatcaccctc cttcatgctc ttccggctga
     1561 gttcacccac ctccctgccg gcagtgcaga tgaagagtgc aacaccctcg gcattcttta
     1621 tctggctgaa gataatcggc ttaacatgaa aaacaacacc ctcaaactca acagttttac
     1681 tgaccgggtc aaggttgaca tgatcgaata tgatatactc tgccctggct tctcctgttg
     1741 ccagaagttc ctcagacagc acggcaataa gatctgtcac cggttcaggt gcggtaccgg
     1801 gcaggtatcc catgacacca gccagggcgt caatgccggt cagaagttca ctgtatctgt
     1861 agcttaatct catttaggca attgtgtcga ctggtagtgc tcaatgaacc tgatcgcata
     1921 gtcatccctc ccaagtacca gatcagcagc tctcaccagc ccggttatct tctcaggatt
     1981 ggcaatgggg cacgtgaggc cgtacagtat tgacatggcc atgtaagcgt tgttgatgtt
     2041 ctcgcggtga ggcagtccga atgagatatt actggctccc tgcgttatgt tgtgtccaag
     2101 cctgtcatga accagcctga tagtctccag ggtaaccctg caggcgttgg ggtcagcact
     2161 gacagccatc gccatcggat caatgatcac atcctcctct tttattccgg atctgactgc
     2221 ccgctcaatt atcttttcgg caatcttcag acgcttttcc ggatcatggg taatgccatc
     2281 atcatcaagc accagtccta ttatggctgc tccgtactcc cgggccaccg gaagcaatct
     2341 ctgcagggaa gcttcttcag cattgacgga atttatcagg cacttgcccc tggctacctt
     2401 cagggcagcc tcgatggctt taggattggg tgagtcaaga cacaggggta tgtcaaagtg
     2461 gtcctgcagt tcaatcaccg cctgcggcag caattcctca tctaccaccc ccggaaagcc
     2521 gacattcacg tcaaggacat ctgccatggc atcaatctga gactttgcaa gctccagcat
     2581 gtaactgaaa tcgttcgcct gaagagttga taccagtttc ttccgccgtg tgggatttat
     2641 gcattccccg ataattacca ccgggccttc ggtatcaatt accacctctt ttaatgttcc
     2701 cttcagtatt gtcttcatta gctgatcatt atggttatat gtaaatatcc ggaagccatt
     2761 tccagaaaat acttcctaag catcgggacc gtaatggttc cgggcgctgc agagcgaaat
     2821 aacaaataat tattttattc aggccagggt tttgagatat tccacggctc cctggggatc
     2881 aggagaatag aaatcggctc ctatttttct gcagaagtcc tccgtgaccg gtgctcctcc
     2941 gacaaggatc ttcatggcag ggaatctgtt cctcaggacc tccacgctct tgcgcatgtt
     3001 ttccattgtg gtggtcagaa gggcagagag tccaatcact gcgcccgggt gtttctcagc
     3061 agcctcgatg aatttttctg tgcttacatc cactcccagg tcgataatct cccagccacc
     3121 accttcaatc atcattgaga ccaggttctt tccgatatcg tgaagatctc ccctgaccgt
     3181 tcctatgata aaagttcccc ttcttttggt ttcgccggag agataataag gtttgatatg
     3241 gatcattgag ctgctcatgg ctttggctgc cataagcatc tggggcacga atatctcatt
     3301 tcttgaaaac ttctgtccca cccttgacat tgcaggtata agggcttctt cgagaatctc
     3361 gccgggtttg attccctcat ccagggcgtg cctggtaagt tcgaaagctc cctcctggtc
     3421 cttcattgca ggtgggtaag gtgattttat gtcaaccttg ccgaattcaa cacaggttga
     3481 gattctttca agtatctcag tcatttcaaa agtcaataac aataagcctt tcctgccctg
     3541 gtggcaggct tttgcaaaga aacacaaaaa atcaggtaaa aagtgcccgt ggacaaagcc
     3601 acggacactt taacctgaac caaccctaat ctaaaacact aatcatatat tcgaataaaa
     3661 tcagttgttg gttgttcaga ccctgagatt ttcatccttt acaggtgatc aatatccagg
     3721 gttctgataa gcagcttttt cagcggctgt catctccagg ccatccagtt ggctttgagg
     3781 tatagggcgt aggtagtgat atttctcaat tgtacgggtc actgcaacag gtgtgtggtc
     3841 tccaagattt gggccacaaa tcgtataagt gagtgccttg gttccccatg tctgtgtacg
     3901 tacaaggtcc caccaccgga caccttcacc ataatactca cgtgaacgct catccagaat
     3961 ataatctaca gttatggttg ctggtgtagc agcaatcata tctgcactat ggtcctcaat
     4021 ttttggagca ttgccgttat tgttccaacg ccactttccg gcacgtgcac gaataacgtt
     4081 gatcagatcc aatgcggttt ttcctgttac tgtatttgca cccttagctg cagcttctgc
     4141 agctatgaaa tagagctctg agaacttggc aattggaaaa ggacgtgtaa tgcttccgtt
     4201 gggttgacca aggccgacac cattatcagt gcggtaagtg ccaatcttgt acccgcaacc
     4261 cggatgtacc agtctgctta ttgcattggg agctattaca taatctgctc ttcccggcaa
     4321 tacaccagca ccaatattac ttttgtaaac cgaattagag taatcgatgc caccaggatc
     4381 actgtcaagg aatgttagta tcgggtcacc cggataaacc atcatatcat tcgcattgta
     4441 caggaacgca tttgtaacgc cacctttatt ccagttgcca cggtacaccg tgacaaaggt
     4501 cccgtcataa cgggaatcat ttgtcttgtc ggcaaatgtg ttggtgaata cttcgatagg
     4561 aggagccata cgagtccagg gacgacccag atgctgttct gcttcacgct gaacagagtt
     4621 cactgttgaa ggattagccc agggagtctt tgagcttgtc atggtggtgt agttccatgt
     4681 catcatccag gcagcgaaat tgtcagcacc gccacctgaa gcataggaga gattcgcgtc
     4741 attccagtag tcacttgcct gggtacggtc ggcatacagc accatttctt tgttacggtc
     4801 gtttgaccca agattgacat cataaaaata tggaagcaat ccgaaaggcc cgggatcatc
     4861 tattgcagct gttgcgacat tataggcctc ctgataatac caggcggcat tatgtccgtc
     4921 agggtcagtg cggggactat ccggatatgt agggatatca tttggatttt ccagccacca
     4981 tgcatatgta aggtaagctt ttgacaggta aaggcgtgca gctgttttgg ttaaagtacc
     5041 tgttaaacgt ccggtagcgg gcaaatcttc ggccgctgcc agcaagtccg gaaatatggc
     5101 tttggtgtaa acttcaggaa cagtatttcg cacagaagtt ctcaccggtg agatgttgaa
     5161 cgccagttca ccagagccaa agtcaagcgg tacccctcca aatgtctgta caagatagaa
     5221 atagtggaat gcccggaaga atctggcttc ggcaataagt gcctcggaaa ttgtccctac
     5281 tgctgtagca ttttcaatga taccatttgc agtgtttata gcagggaatg cattagacca
     5341 gagccctccg gcatctgagg cgctgggagt gataggcccg cctgccccgg aaaggtccat
     5401 gatcttgaag ttacctgtac ctccgctggc agcccaagtt gcttcatcag tgccggtcag
     5461 gccattactg taaaagtatg catcgcccca taaacgtctg agtgtggcgt attcagctgt
     5521 tagacccccc ataacgcctt ccggtgtttt gaaagtctca ggtgtatata tagcacgtgg
     5581 ctgctcctcc agaatatcag agcacgacgc aaacaggagc aacgcaatgg ctgttcctaa
     5641 aacagaattt atcagtttac gtttcattat tgttcctttt taaaatgaca tattgatacc
     5701 gatcatgtag ttgcgtgttg caggcgtgtt ggtgcctatg gtcagcagtc gtctcagtga
     5761 tccggacaag ggcactgcag cattttcatt accgtacgag ttagtctcgg gatccatccc
     5821 tgattccttc gtatacggag agaacaatac gaacggattc tgtacggttg catataccct
     5881 cagtgtctta aatccagctc tttttatcca ctcgttgcta tccagatagt acccgagtgt
     5941 aattgtccgc accttcaaat acgatgcatc aaagtagccc attgtgcttc catacttagg
     6001 attgtcgcca ccaacaccgt ttggtttggg atacctggcg tctgtgttat ccggggtcca
     6061 gtagtctatc cttacgttat tgcccgatct tgtgttcagg ttgttcaggt agcttccgga
     6121 tccgtaaaga acgctgttga gaataccgcc tttcttgaag gcaccaataa tggagaggtc
     6181 tactccttta tatgccagac gggtattgaa gcctcccata aagtcaggct ccatttcaat
     6241 tatttgcctg tcagcagctg taattgctct tgcaggagtg acaccatcgt taaggtaatc
     6301 accggtatat tttaccttaa tcataccaat attgccgccc ggttcaagaa tattgaagta
     6361 tggatcattc tgctgccaga taccaatatt ctcataatca tagataacat caataggatg
     6421 gcctacaaac caccagttgg tttcatcctt caattgtccc gaagcaagct caaccagctt
     6481 gttacgattg gcgtacaggt tgacacctgc ttcccatgtc catccgttta ggttgtcaat
     6541 aatcacaccg tctactgaaa attcaagacc tttattctca gtctttccaa tattggccat
     6601 atagctgcct acgccagaag tggatggcag cccgacacta agaataacat catgggtata
     6661 cattgcgtaa tactcaacag ttcctgacag tcgattcttc aggagtccaa agtccagacc
     6721 aatattaaat gtctttgaga attcccatcc taaagtaggg ttaggcagtt ctgaaacata
     6781 catcccgata gaataaacag tgccgaaatt ataaggtctg gtgtttaaca gaccaagggt
     6841 tttgtaagga tctacagatt ggttggaagt ttcaccgtag cccatcctca gtttaagaag
     6901 atttatctgg ctgatgctct tcatgaattg ctcatttgca atattccatc ctaccgacac
     6961 ggcaggatag gtatgccact tctgtcccgg agccagtctt gatgaaccat cagtacgggt
     7021 ggcaacactt atcatataac gactgtcata tgaatacatt acacggccca tgtatgataa
     7081 caaacctgtc agttcatatc tctggtttga aggattgaca gtcttatccg cagtggcctg
     7141 tcccaggttg tagaattgga atgcatcgat gggaatatct ttggttgaaa catttgaaga
     7201 gttatacata gattgctcag ctgagaacat tgccactgca ttcaactgat gttttcccgc
     7261 aaaggtttta tcatatgtca gcaggttctc ggctgcccag ttaatcgtat gagagttgct
     7321 gattgaggca gtggagttat tggatggtgt accgctgaaa actcctacac ctgtatagct
     7381 tccattgtta ctctgccggt agttcaaacc caggttaatg cgatacttaa gaccatctat
     7441 ccctggtatt ttcacttcac cataaagtgc attgtaagtt ccgaacgccc tgttctgatc
     7501 gatatattta tctcctaaac cttccaatac ctctcttgtc gatacccact gagcacccga
     7561 agtggcctgc atgaatcttt gtttcagaga gccatcttcg ttgtagatgt tggctatagg
     7621 tgaatgatcg agggccattc ctacagctcc gaggttattc ccgttcgtaa ctgagtaatt
     7681 ggtatttgtg ctgataccga aacgtaagta tttcccgatt ccctgatcaa gtgaactccg
     7741 gattgaataa cgtgtgtaat cctgaagtgg aacaacggcc tcgtccttgt agtatccaac
     7801 accgaacgtg tagtttcctg tctgtgttcc gccggttatg cccaaatcat gactgttcac
     7861 cattccggtt ttatacagca gatcctgcca gtcagtatcc atatcattag attcatccag
     7921 cgtgttagtg tactggttag ctgcagcacg cagcgcaaca aattccggtc cgttcatcat
     7981 tgggtatttg gcaaacacct gcttcatccc aacatagctg tcgaggctta tccgtgcctg
     8041 ctgaccggca tagcctttat tggtggttat aaggattact ccgtttgctc ctcgcgaacc
     8101 atagatggct gttgaagaag catccttaag aatatcaata ctcttgattg cgctggggct
     8161 aatgtcaccg attgatcctg caaagggtat cccgtctatt acaaccagcg gatcattgct
     8221 ggcattaagt gaacgagtac cacgaacccg tatttgcatt gttgcaccag gtttggttga
     8281 tacctgtgac atatcaacac ctgagacacg cccttgaaga gcctgtgaaa tatttgatga
     8341 cggtacatct ctgattgcat caccgcctat tgaagctacc gatccggtca catcgctttt
     8401 cctggctgtt ccgtaaccaa taactacaat ttcttcaagg cctatgtcac ttgcagccag
     8461 ggctaaattg acaactgcct gggatccgac tgtaacttcc tgagtcgtgt atccgacgaa
     8521 agagaataca agcacaggat tggctgtcgt caccactatt ctgtattggc cgttcgcatc
     8581 tgaaagacta ccgttgttag tacccttttc catgatagtt acccctggca aggggttgcc
     8641 atcgctctca gtaatgagtc ctgtaacgac tctttgctga ttcagattca gttcctcagg
     8701 attggatcct gaggcaaatg ccatccctgt ctgcacaaga atcagcattg caatgagcgt
     8761 aatgctcaaa agtagttttc caaagtattt ctttagcaga ataccttggg tcaggggttc
     8821 attaatcata cgatttagat tttaaggtta aattttttga tgttggtttg gctgggatga
     8881 ctactctggg tagtcatctc atcagagggt tcaggaatgt tcttcccgtt tcagggtgat
     8941 ttgtcattga ttcgcatggt tgtttaaaag gtctgtagtt tatatgtcaa ttaagcttgg
     9001 ccaaccaaca ttcagcaccg tgccgggaaa acggttcttt aaactggaaa accttattcc
     9061 ctgtcactat taaaatcaaa accctgcaat actacccttg gaaaatggtc tacaacatgg
     9121 taaatataat aaataatttt taaaatgcaa ccgatttcat aaaaataaaa tctattcaca
     9181 tcaggttcag attcattaaa ttcacctgat atgaaacctt gcccgtagag actgtttttt
     9241 tcttgttttc gattttatga ctatttttag aatcgctgac gaaaaatcaa aggtaaaccc
     9301 gacgtttatt tatttcataa ataagaactt atctaatgtt taacatgatt ataaggatat
     9361 gaaaagaaca cagtttttac ccatttgcat gatcatcttc ggttttgttt ccgggaatct
     9421 tttttcccag aatccaaccg tgatcaaaat tgatcctgac cgcacgatag caacagttga
     9481 tccgaatatc tacggagctt ttgttgagcc tatacgtaca gtggtatacg gcaacatcta
     9541 tgatcccgga tcttcttttg ctgatgaaaa cggtttcagg aaagacgtca ttcagttgtt
     9601 acgtgagctg aaaataccgg ttgtgagatg gcccggcggc aattacgtgt ctggatataa
     9661 ctgggaagat ggcattggcc cgaaggacca gcgcccggca agactcgacc ttgcctggca
     9721 tcaggttgag accaaccaaa tgggaacaga tgaatacgtc aagctttgca aactcattgg
     9781 ctcggagaat tttatctgca taaatgccgg gactggcacc cttgaccagg caaggcactg
     9841 ggttgagtac tgcaacattg aaaaaggcac ctggtattct gacctccgga gaaagtatgg
     9901 caatgagaca cctttcaaag tcaaatactg ggcactggga aatgagatcg acggcccatg
     9961 gcagatggga caaaaaagtg cagaagacta tgtgaagttc gcccttgagg cagctaaact
    10021 gatgcaatgg gtagataaag acatcaagct catcgccagt ggcgcctcaa attataaacc
    10081 cgataacaac tggatagact ggaatgacca tgtactgacc aaaatggcag gaagcatcga
    10141 ttacctctca gtgcacaggt atgcaacaga ggccctggga aacgacagga gcttctcagg
    10201 tatgatgtgc cttggccttg atctcgatca gaaaatagag acagtaaaag cccttatcct
    10261 gaaggcgatg atgaaaaccg ggtcaaaacg gcctgtatat atctcatttg atgagtggtc
    10321 cgcaggattc gggaacagca tcacggcttc actgatggtg gcacagcacc tcaactcctt
    10381 tatcaggcat gccgatattg tgaagatggc aaatataacc atgctttcaa gcctcgtggg
    10441 atattctccc gagggcgctg ataaaaatgc attgttcaaa gcgttttacc tgtacagcaa
    10501 taattgccac ggaaaagccc tcgatgtcta tgccagctgt gaaaaataca gcaatgatgt
    10561 attcaaagac attccttatc tcgatgtcac agccgttctg aatgatgcta ccggaaccat
    10621 tgtgttaaat gtggtcaaca ggcatgaaac aaaccccatc acggcagaca tagttttaca
    10681 gacgggagaa tttaccggaa gtgcaatagc caatgaggtc aacgggaaaa ttgttgtccc
    10741 cggcaatccc agggcttcag agccggtaaa cagcacttca gctgacataa aattcaaggg
    10801 caatacagtt aagtatactt tcccggctca ttcattgaca cagcttatgc ttcccctgaa
    10861 gtgaaaagta ctgttgtcaa tggtcagaag gttgaccatt tgcagcttaa atcaggaccc
    10921 tgccggattt taggtatatg ttgccccggg catctatggt caatccatct gaaccggatg
    10981 gtgcaaa
//
LOCUS       DAOPXU010000092        10960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_6877_length_10960_cov_11.685465, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000092 DAOPXU010000000
VERSION     DAOPXU010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10960
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6877_length_10960_cov_11.685465"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1361
                     /locus_tag="PKK03_09270"
     CDS             120..1361
                     /locus_tag="PKK03_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX84630.1"
                     /translation="MKGLTLFWENVRVAFKAIRSNRVRAALTMAIIAFGIMALVGILT
                     AIDGLKSTLTEQFTMMGANSFTITSRSMRITFGDQHIRTKNHSHITFKEALAFKERFR
                     EPAWVSVSFNASGMAEYRFGNNKTNPNISILGSDENYLTIAGQELESGRNFSQEDLAG
                     SRHVVIIGKDIVRFLFPGVDPLGKEISLPGLKLTVIGVIKSSGESMMGTDQVTIIPIT
                     TARQYFSRPNISYNIGVMPQKSVSLDMMASEAEGIFRIVRNLDPRDESDFNVQKSDSL
                     IEMLMENIKYVTLAATIIGLITLFGAAVGLMNIMLVSVTERTREIGVRKALGARKSVI
                     RQQFLYESVIIGQMGGLLGIITGILIGNLVSLGMGSSFTVPWVWVIGGVLACFLVGIA
                     SGYFPAVKASAVDPIEALRYE"
     gene            1412..1789
                     /locus_tag="PKK03_09275"
     CDS             1412..1789
                     /locus_tag="PKK03_09275"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaC family regulatory phosphoprotein"
                     /protein_id="HNX84631.1"
                     /translation="MSIEIIQTGSNNIPRITYDQDEDVLSITGRSIAERPDIIYTPLE
                     TWIRSHLERFTTLRIVIFLEYINSGSSKALRDVLRMISSYRAPRYRIRITWLYEEDDE
                     AMHELGEHYRDSAGIKMDVQMVL"
     gene            complement(1910..2920)
                     /gene="gap"
                     /locus_tag="PKK03_09280"
     CDS             complement(1910..2920)
                     /gene="gap"
                     /locus_tag="PKK03_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNX84632.1"
                     /translation="MAKIKVAINGFGRIGRNVFKMAFEKSNIEIVGINDLTDTKTLAH
                     LLKYDSTQGQFPGVTYDEQNIIVRGDKIKVTAEKNPAAIPWEVKPDIVIESTGIFTSK
                     ESPKGGYGDHLKNGAKKVILTVPAKDTIDAMIVAGVNDDVLKPEHTCVSNASCTTNCL
                     APVAKVLNDNFGIEIGYMTTIHSYTNDQRILDLPHKDLRRARSAALSQIPTTTGAAKS
                     VGKIIPELKGKLDGISVRVPTPTGSLVDLVAVLKKAATKEEINAAMKKAAEGPMKGIL
                     EYTEDEIVSVDVIHNPASSIFDAQSTMVNGTMIKVLSWYDNEWGYSARCVDLIEMMGK
                     ML"
     gene            complement(2999..3793)
                     /locus_tag="PKK03_09285"
     CDS             complement(2999..3793)
                     /locus_tag="PKK03_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84633.1"
                     /translation="MIKRFWHVILFIAICGSLAGQGDIDPQDRIFWRNEKSIGPALNA
                     DGWSIIYRDLRQFKPNQRWFIEGSVEGFKHPKEIKLSNYYFQSPGSFVFGKLNTTWTL
                     GAGAGYQLEIFEKHDLGGVSVSWFGGGGATLLFAKPIYYKIIVLLGNDYYTIEEQKFD
                     LTIHQPLDIMSKASFFKGFDEIKLYPGLYARTGFNFEYSRNDRITHTVEVGASLHAFA
                     KTIPIMASDDNKQFFPSIFVGYRIGMILDPVSQRGLFDLLRKQSAE"
     gene            complement(3837..3962)
                     /locus_tag="PKK03_09290"
     CDS             complement(3837..3962)
                     /locus_tag="PKK03_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84634.1"
                     /translation="MKKVGLIVVLALLAVLAFSSCNREACPAYSSADAASTEQEG"
     gene            4146..5030
                     /locus_tag="PKK03_09295"
     CDS             4146..5030
                     /locus_tag="PKK03_09295"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HNX84635.1"
                     /translation="MEKESLSQEKPAYNDLSAQESKVIISKATEYPFTGLYEKFNAKG
                     TYICKQCGNALYHSDAKFDAGCGWPSFDEEVAGAVKRVSDADGMRTEIICNSCGGHLG
                     HVFTGERFTPKNTRHCVNSVSLDFVPEVLPAGTYGTALFAGGCFWGVEYFLQKEPGVV
                     AVVSGYTGGHIKNPSYREVCNGNTGHAETVKVMYDTKKTSYDKLVKLFLEIHDPTQVG
                     RQGPDIGDQYRSEIFWKNEEQRKTAAKYLTLLRDKGLKVATGLTKASEFYPAEEYHQD
                     YYFRNGKMPYCHGYTRRF"
     gene            complement(5200..8499)
                     /locus_tag="PKK03_09300"
     CDS             complement(5200..8499)
                     /locus_tag="PKK03_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84636.1"
                     /translation="MKKVLTLIFVLLLAGSGSLWAQKKETKKDAKTPESQIKPSVSSS
                     FFSAVSFRSVGPAWASGRIADLAVNPSNHSEIYAAVASGNIWKSENNGTTWEPIFDKY
                     GAYAIGCIVIDPGNHNVVWAGTGENNHQRALGWGDGVYRSDDAGASWKNMGLKESRQI
                     GGIAIDPRNSDVVYVAAEGSVWGPGGERGLYKTADGGKTWEKILNISENTGINNVVLD
                     PRNPDVIYCTSEQRRRHVGMKISGGPESAVYKSTNAGKSFEKIMKGLPGGDLGGMGIA
                     ISPVNPDVLYLIVEAQGEAGGFYRSVNRGASWQRMNSYHSSGQYYNKIICDPKDVDRV
                     FSLDTYTQVTVDGGRTWSRVSNNGRHVDDHALWIDPTNTDHFYIGGDGGIYETYDNGS
                     NFIFKSNLPVTQFYRVAVDNSYPFYYIYGGTQDNNSMGGPSASTSNGVVNDDWFVTSG
                     GDGFWVAIDPEEPDIVYTESQYGNASRYDRRSGESVSIKPFPGEAELTYRWNWDTPIL
                     ISPHNNKRIYMAANFLFRSEDRGSSWKTISPDLTRNENRDNFKVMDRYWPSDAVAKNK
                     STSLWGTIVSLTESPVQKDLLYSGSDDGVIAVSEDGGANWRMNKTYPGVPEYTYVSDL
                     LADKYNADVVYATFNNHKRDDFRPYVLKSTDKGRTWTSISANLPANGPVHTIEQDHVN
                     PNLLFLGTEFSFFVSFDAGATWTEFNRGLPTIAVRDIAIQERENDLVIATFGRGFYVL
                     DDYTPLQNYSKSITTKAGYIFPIKDARMFVQTDEFDNQGSMYYVAKNPPYGATITYYV
                     DTVPKTARDIRKEKEKKLVEKGDFIPQPTARELSLEEREIKPYLIFTISDEEGSVIRK
                     LYKNASKGVGRVTWNFTYAGYQPVRASGKFSPVADEGRGRWGGGGISVIPGKYFVSMA
                     MYADGEIKELAGPEPFVCKPLDIVTLPATNAAAKEKWMKEMAAFAKTAYGAISYATEL
                     DGTVNTIMQTIHQMPEAPASLMKEAAALSDEMSEITYLIRGISVGASTEETPPSPVSL
                     SARLSAMSRSLYGNSGDISGIADQQFTILKKEFPVLLERIQKAGKAVDALNAKLDEIK
                     APWTTGRVPRL"
     gene            8610..9353
                     /locus_tag="PKK03_09305"
     CDS             8610..9353
                     /locus_tag="PKK03_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNX84637.1"
                     /translation="MNSTYFTRRTIRKYAQRDIDSDLLERLLTAGTRASTTGNMQIYS
                     IIVTRDQEMKKRLAPAHFNQPMVTEAPVMLTFCADFNRFNLWCRQREAEPGYDNFMSF
                     MTAAIDSLLAAQTICNAAEEEGLGICYLGTATYNADRIIEVLELPLGVVPVATVTLGW
                     PAQVPEQPDRLPLEAVVHYEKYHDFTPDLIDSLYREKEARTDSQQFIAANGKETLAQV
                     FTDVRYKKADNEYFSGKFLEVIRRQGFTF"
     gene            complement(9343..10416)
                     /locus_tag="PKK03_09310"
     CDS             complement(9343..10416)
                     /locus_tag="PKK03_09310"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HNX84638.1"
                     /translation="MYDHKYLLDFTTEVFTRVGCNSDDAAAVAAVLVAAELRGHSSHG
                     MIRVREYYELWKSGRVNVSPDVKVVHETPSTAVVDGDRCFGMVAGVRSMHLAIEKAAA
                     TGTGWVAARNSNHFGIAGYYAMMALEHDMVGIAVTNANPLVVPTFSVSRLLGTNPLAV
                     AVPALEEPPFVADFATTPIARGKLAVSEKKGEKVPLGYVQDAEGNPSDDPGILRHGGG
                     MVTLGGDRLHGSHKGYCMTAVIDIFSALFSGANFGPFVPPSVAYLPVPENQPGKGTGH
                     FFGAMRIDAFRPANEFREAMDLWIRTFRNAKPAKGEQRVLIPGDPEREAEKEHLKSGI
                     SVVPAVVDDLKIIATELGFDFRK"
     gene            complement(10430..>10960)
                     /locus_tag="PKK03_09315"
     CDS             complement(10430..>10960)
                     /locus_tag="PKK03_09315"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX84639.1"
                     /translation="QANRKAVIIDERFNGGGMIADYYIDLLQRPLQSYWNMRYGNDLK
                     TPSASIQGPKVMIIDETAGSGGDMLPWMFRKFGVGTLVGKRTWGGLVGTLGFPELMDG
                     GMVTAPNLAIWTEDGFVVENVGVAPDIEVEQWPADVLEGRDPQLEKAIEVALKELEKN
                     PPAEPVRPPYPVKVRK"
BASE COUNT         2573 a         2839 c         2806 g         2742 t
ORIGIN      
        1 gttcaactga cagatggtcg ttcttcaccc gggcaaaggt aaaaaatgaa gtgaatgtta
       61 aagaagaaga aattcagaaa agatgtatct tagttccatc tttatctgaa ggaagtaccg
      121 tgaaaggatt gactttattc tgggagaatg tcagggttgc attcaaggca atcaggtcaa
      181 acagggtcag ggccgcactg acaatggcta taattgcttt cgggatcatg gcccttgtgg
      241 gaatactcac tgccattgac gggttgaaga gtacccttac cgagcaattc acaatgatgg
      301 gggcaaactc cttcacaatc acctcacgct caatgaggat tacctttggt gatcagcata
      361 tccgtaccaa aaaccattcc catatcacct ttaaggaggc tcttgccttc aaggagaggt
      421 tccgggaacc ggcctgggtc tcagtttcat tcaatgcatc cggcatggcc gaataccgct
      481 tcggtaacaa taagaccaat cccaacattt caatcctggg ttcggatgaa aactatctga
      541 ccattgcggg acaggagctt gaatcagggc gcaacttctc acaggaagat ctggctggca
      601 gcaggcatgt ggtcattatc gggaaggata tagtccgctt cctctttcca ggtgttgacc
      661 ctctcggcaa ggagataagc ctccccggcc tgaaactcac ggttatcggg gtgatcaaga
      721 gctccggcga aagcatgatg ggcactgacc aggtgaccat tatccccatt accactgcca
      781 gacagtactt ctcaagaccc aatatttcat acaatatagg tgtgatgcct cagaagtcgg
      841 tcagtcttga catgatggca agcgaagccg agggtatctt caggatagtg cgtaacctcg
      901 acccccggga tgagtctgat ttcaatgttc agaagagcga ctccctgatt gagatgctga
      961 tggagaacat caagtatgtg acgctggctg ccaccataat cggacttatt actctattcg
     1021 gggcagctgt gggtctgatg aacatcatgc tggtatccgt tacggagcga acaagggaga
     1081 ttggtgtccg aaaggcactc ggggcgcgaa aatcagtgat caggcaacaa tttctttatg
     1141 aatctgtcat tatcgggcag atgggtggcc tccttggcat tataacggga attcttatcg
     1201 gtaacctcgt ttccctcggg atgggttcaa gtttcacagt gccctgggtg tgggtcatcg
     1261 gaggagtgct ggcctgtttc ctggtcggga ttgcttcggg atacttccca gccgtaaagg
     1321 catcggcggt tgacccgatt gaagcgcttc gctatgaatg acgtactgac tatctgatga
     1381 gcagcactgt aatgatgcgg gagtttgaaa gatgagcatt gagataattc agacaggatc
     1441 gaacaacatt cccaggatca cctatgatca ggatgaggac gtgctgagca tcaccggacg
     1501 ttcaattgct gagaggcctg atattatcta cactcccctg gagacatgga tcaggtcgca
     1561 cctggaacgg ttcaccacgc taaggattgt cattttcctg gaatatatca acagcggatc
     1621 atcaaaggcg ttgcgcgatg tgctcaggat gatcagcagc taccgggctc cgcgctacag
     1681 gatacgcatc acctggctct acgaggagga tgacgaggcc atgcatgagc tgggggaaca
     1741 ctatcgcgat tcggccggca ttaaaatgga tgtgcagatg gtgttgtaac cgtgggtggg
     1801 atcatcttcc ggattcaaat catgtttata taaacgaaac gtggggtctg agaccccacg
     1861 ctacatcata ttattgcgtc tcctgcctgt gtggcaggtt cagcggattt taaagcattt
     1921 tacccatcat ctcgatgagg tcaacacacc ttgccgaata accccactcg ttgtcatacc
     1981 atgacagaac cttgatcatc gtgccgttga ccatggtgct ctgagcatca aatatcgatg
     2041 acgccgggtt gtggatgaca tcaacggaga caatctcgtc ctcggtatac tcaaggatgc
     2101 ctttcatcgg accttcagca gccttcttca ttgctgcgtt gatctcctcc ttcgttgcag
     2161 ccttcttcag aacggcaaca aggtcaacca gtgagccggt gggcgtaggg accctcactg
     2221 agatgccgtc aagcttgccc ttcagttcgg gtatgatctt gccgactgac ttggctgcgc
     2281 cggtagtggt aggtatctgt gagagggcag ctgacctcgc cctgcggaga tccttgtgcg
     2341 gaagatcgag gatacgctgg tcattggtgt atgagtgaat ggtggtcata taaccgatct
     2401 cgataccaaa gttgtcattc aggacctttg ctaccggtgc aaggcagttc gtggtacagg
     2461 atgcatttga tacgcaggtg tgctccggtt tgaggacatc atcatttaca ccagcaacta
     2521 tcatcgcatc aatggtatcc ttcgcgggaa cagtcagaat aaccttcttg gcaccgttct
     2581 tcaggtgatc accgtaaccg cccttgggac tctccttcga tgtgaatatt ccggttgatt
     2641 caattacgat atcaggcttg acttcccaag gaattgctgc gggattcttt tctgcagtga
     2701 cttttatctt gtcacccctt actataatat tttgctcatc ataggttact cctgggaact
     2761 gaccctgagt ggagtcatac ttgaggagat gagctagtgt ctttgtgtca gtaaggtcat
     2821 tgattcctac tatctcaatg ttggacttct caaaggccat cttgaaaacg ttacggccaa
     2881 tcctgccaaa tccgttaatt gctactttaa tctttgccat tgctataatg ttaataaggt
     2941 tactaaaacg aatgacaaaa gtacaaaata aaaaggagag gcaaacaatg agcgataatt
     3001 attctgcgga ctgctttcgg agaaggtcaa acagaccccg ctggctgacg ggatcgagta
     3061 tcatgccaat acggtaaccg acaaatattg aggggaagaa ctgcttgttg tcatcgctgg
     3121 ccattatggg aatggtcttt gcaaaggcat gaagtgaggc acccacctcc acagtgtggg
     3181 ttatccggtc attcctgctg tattcgaagt tgaacccggt gcgtgcatag agtcccgggt
     3241 aaagttttat ctcgtcaaaa cccttgaaga aggaggcttt gctcatgata tcgagtggct
     3301 gatggatggt cagatcgaat ttctgctcct ctatcgtata gtaatcattt cccagcaata
     3361 ctattatctt gtaatagatt ggcttggcaa agagcagggt tgctccgcca cctccgaacc
     3421 agctgaccga aacccctccc aaatcatgtt tttcaaatat ctccagctgg taaccggccc
     3481 ctgccccgag ggtccaggtg gtattcagtt tcccgaacac aaatgaaccc ggactctgga
     3541 aatagtaatt tgacaacttt atctccttgg ggtgcttgaa gccttcgaca cttccttcaa
     3601 taaaccagcg ctgattgggc ttgaactgac gcaggtcacg gtatatgatc gaccagccgt
     3661 cagcattgag tgccgggcca atcgattttt cattgcgcca gaaaatcctg tcctgcgggt
     3721 caatatctcc ctgccccgcg agagacccgc agatagcaat aaaaagtata acgtgccaaa
     3781 atctcttaat cattattgcg ctgatgttaa gactggcagc gggggagaag cgatgatcag
     3841 ccttcctgct ccgttgaagc agcatcggca ctgctgtatg ccggacatgc ttcacgattg
     3901 catgaactga atgccagtac tgcaagcaga gcgaggacga caattagacc aacttttttc
     3961 ataattcctg cctttaagtc tgagacaaaa gtaactttat ttttaaaaca agatttgtgc
     4021 catattatta tttaagtctg ctcatttttt aaaccaacag ctctgtcagt tgttatctgg
     4081 taaactgaaa aacaatgaaa tttcagggat atataatact tttaatacca atatttatac
     4141 tttcaatgga aaaggaaagc ctgtcacagg agaagccggc atacaatgac ctgtcggcac
     4201 aggaatcgaa ggtaataatc agcaaggcaa cggaataccc cttcacgggg ctttatgaaa
     4261 agttcaatgc caagggaaca tacatatgca agcagtgcgg caatgccctc tatcactctg
     4321 acgccaagtt tgatgccggc tgcggatggc caagctttga cgaggaggtt gcaggtgcgg
     4381 tgaaacgggt ctcagacgct gacggcatgc gcaccgagat aatctgcaac tcatgcggcg
     4441 gacacctcgg tcatgtcttc accggagaac ggttcacccc taagaataca cgtcactgcg
     4501 tcaattcggt atctctcgac ttcgtaccgg aggtactgcc ggcaggtact tacggcactg
     4561 ccctctttgc cggcggatgc ttctggggag tcgagtattt cctgcagaag gagccggggg
     4621 tggtggcagt cgtctccggg tatacagggg gtcatataaa gaatccgtct taccgggagg
     4681 tatgcaacgg aaataccggc catgccgaaa cggtgaaggt catgtatgac acgaagaaga
     4741 cctcgtatga taagcttgta aaactcttcc ttgagataca tgatcctacc caggtgggaa
     4801 gacaggggcc ggacatcggc gatcagtacc gctcggagat tttctggaag aatgaagagc
     4861 agcgcaagac tgcagcaaag taccttaccc tgctgcgcga taaggggctg aaggtggcta
     4921 ccgggctcac caaagcgtct gagttttatc cggcagagga gtatcaccag gactactatt
     4981 tccggaatgg taagatgcca tactgccacg ggtataccag gaggttttag tcggcagtac
     5041 cagttggcag ttggcagtca gtcagatatt tgggaactgc agactgcaga atgaaaaaaa
     5101 ccccgacagc cgtcaggcat gtcgggggtt cagattattc gcaattttca gttcgcaact
     5161 cgcccaattc gcaattcgca aatcgcaaat cgcaattcct tacagccttg gcaccctccc
     5221 ggttgtccac ggagccttta tctcatccag tttcgcattg agcgcatcaa cagcctttcc
     5281 tgctttctgt atcctctcaa gcaacaccgg aaactccttc ttcaggatgg tgaactgctg
     5341 gtcagcaatt ccggagatat caccggaatt tccgtagagc gaccttgaca tggctgacag
     5401 ccttgcagag agtgataccg gtgacggagg agtctcctcg gtgctggcac caacagaaat
     5461 tcccctgatg agatatgtta tctcactcat ctcatcactc agcgctgcag cttccttcat
     5521 cagtgaagcc ggtgcttctg gcatctgatg gatggtctgc ataatggtgt taacagttcc
     5581 gtcaagctcg gtggcatatg agatggcacc gtaagcagtc ttcgcgaagg cagccatctc
     5641 cttcatccat ttttccttgg ctgccgcatt ggtggccggc agggtcacta tgtcaagcgg
     5701 cttgcacacg aagggctcgg gaccggccag ttctttgatc tcgccatcgg cgtacatagc
     5761 cattgaaaca aaatactttc ccggaatgac cgagatgcct ccgcctcccc agcgtccacg
     5821 gccttcatca gccacagggc tgaatttccc ggaggccctg acaggctgat aaccggcata
     5881 ggtaaagttc catgtcaccc ggcccacacc ctttgatgca tttttataga gcttgcggat
     5941 caccgagcct tcttcgtcac ttatggtgaa gatgagatag ggtttgatct cacgctcctc
     6001 aagactgagc tcccttgctg tgggctgcgg aatgaaatca cctttttcga cgagtttctt
     6061 ctccttctcc ttcctgatat cacgggcggt cttcggaaca gtgtcaacat aataggtgat
     6121 ggtagctccg tagggtggat tcttcgccac ataatacatt gagccctggt tgtcaaactc
     6181 atcagtctga acaaacatcc tggcatcctt gatggggaag atataaccgg cctttgtggt
     6241 gatgctcttc gagtaattct gcaacggagt gtagtcatcg aggacataaa atcctcttcc
     6301 gaatgtggca atcaccaggt cattctcacg ttcctggatg gcaatgtcac gcactgcaat
     6361 tgtcggaagg cccctgttga actcagtcca tgtggcgcct gcgtcaaatg atacgaagaa
     6421 gctgaactca gttcccagga acaggaggtt gggattgaca tgatcctgct cgatcgtatg
     6481 aaccggtccg tttgccggca ggttggcgct tatcgaggtc catgtgcgtc ccttgtcagt
     6541 gctcttgagt acatacggcc tgaaatcatc acgcttgtgg ttgttgaatg tcgcatagac
     6601 tacgtctgca ttgtatttgt cggccagcag gtcgctcaca taggtatatt ccggcacgcc
     6661 gggataggtt ttgttcatcc tccagttggc gccgccgtct tccgagactg caattactcc
     6721 gtcatcactt ccggaataga gcaggtcctt ctgtacgggc gattcagtga gtgaaacaat
     6781 tgttccccag agggaggtag acttgttttt cgcgacagcg tctgacggcc agtacctgtc
     6841 catcacctta aagttgtctc ggttctcgtt ccttgtcagg tcgggcgata ttgtcttcca
     6901 gctgcttccc cggtcttcac tgcggaacag gaaattggca gccatgtaga ttcttttgtt
     6961 gttgtgcggg ctgataagga tcggagtatc ccagttccag cggtaggtca gttcggcctc
     7021 accggggaag ggcttaatgc ttacgctctc gccgcttctg cggtcataac gggatgcatt
     7081 gccgtactgc gattcggtat agacgatgtc cggttcttcc ggatcgatgg ccacccagaa
     7141 tccgtccccg cctgaagtaa cgaaccagtc gtcattgacg accccgttgc tggtgctggc
     7201 cgaggggcct cccatggagt tgttgtcctg ggtaccgccg tagatgtagt agaaggggta
     7261 tgaattgtca acagccacac ggtagaactg ggttaccggg aggttcgact tgaagatgaa
     7321 gttactcccg ttgtcatatg tctcgtagat gccgccgtca ccgccgatgt aaaagtggtc
     7381 agtgttggtc ggatcaatcc acagggcatg gtcgtcaaca tgacggccgt tgttgcttac
     7441 cctcgaccat gtgcggccgc catcaacagt cacctgggtg taggtgtcaa ggctgaatac
     7501 cctgtccaca tccttcgggt cacagatgat cttgttgtaa tactgtccgc ttgaatggta
     7561 ggagttcatc ctctgccatg aagcgcctct gttgaccgac ctgtagaagc caccggcctc
     7621 accctgtgct tccactataa gatagagaac gtcagggtta accggcgaaa tggctatccc
     7681 catgccgcca aggtctcctc ccgggagccc tttcattatc ttttcaaacg attttccggc
     7741 gttggttgac ttgtagactg ctgattccgg tcctccgctg atcttcattc cgacatgtct
     7801 gcggcgttgt tcgcttgtgc agtaaatgac atcgggatta cgcggatcaa gcaccacgtt
     7861 gttgatgccg gtgttctcgc tgatattgag aatcttttcc catgtttttc cgccgtcagc
     7921 cgttttgtac agtccgcgtt caccgcccgg accccataca gatccttccg ctgcgacata
     7981 taccacatcg ctgttgcggg ggtcaatggc tattccgcct atctgacgcg attccttcag
     8041 acccatgttc ttccagcttg cgcctgcgtc atccgagcgg tatacgccgt caccccatcc
     8101 cagtgcacgc tgatggttgt tttcgccggt accggcccag accacgttgt ggttgcccgg
     8161 gtcaatgacg atgcagccaa tggcatatgc accgtatttg tcaaaaatcg gttcccaggt
     8221 ggtgccgttg ttctcgctct tccagatgtt gcccgaagca accgcggcgt agatctccga
     8281 atggtttgag ggattgaccg cgaggtctgc tatacggcct gaggcccatg ccggccccac
     8341 ggaccggaaa gaaacagcgc tgaagaatga tgaggatacg gagggcttga tctggctctc
     8401 cggtgtcttt gcatccttct tcgtctcttt cttttgtgcc cacagactgc ctgagcctgc
     8461 cagcaacaat acaaaaatca gtgtcagaac ttttttcata agaggttttt tggtcagttt
     8521 attactatcg ctaaggtaag ataaggaatt ttgtcagcta actttttaac gttatttaat
     8581 tattttgctg cagattaaaa agagtctaaa tgaacagcac atattttacc aggagaacga
     8641 tacggaaata cgcacagcgc gatattgaca gtgacctcct tgaacgactg ctgacggcag
     8701 gaacaagggc atccactacg ggcaacatgc agatatacag catcatagtg acccgggatc
     8761 aggagatgaa aaagcggctg gctcccgccc atttcaacca gcccatggtg acagaggcac
     8821 cggtgatgct taccttctgt gccgacttca acaggttcaa cctctggtgc aggcagcgtg
     8881 aggccgagcc cggctatgat aatttcatgt catttatgac tgcggcaatt gattcacttc
     8941 tggctgccca gacaatctgc aatgccgctg aagaggaggg actgggcatc tgctatctcg
     9001 gaacggctac ctataatgca gacaggatca ttgaggtgct ggaacttcca ctgggcgtag
     9061 tacctgttgc cacggtcaca ctgggctggc cggcacaggt gccggagcag cctgacaggc
     9121 tgcccctgga ggcagtggtg cattacgaaa agtaccatga ttttactccg gacctgatcg
     9181 acagcctgta cagggagaag gaggcacgca ccgactctca gcagttcatt gccgcgaacg
     9241 ggaaagagac gcttgcacag gtcttcacag acgtgcgtta caagaaggca gacaacgaat
     9301 atttttcagg gaaatttctg gaagtgatcc gccgtcaggg tttcactttc tgaaatcaaa
     9361 tcccagttca gtggctatga tcttcaagtc gtcaaccacg gcaggcacca cacttattcc
     9421 tgatttaagg tgttccttct ccgcttccct ttccggatca cctggaatca gcacacgctg
     9481 ttcccccttt gcaggtttcg cattgcggaa tgttctgatc cagagatcca ttgcctccct
     9541 gaattcattt gcgggcctga aggcatcaat tctcatggcg ccgaaaaaat ggccggtacc
     9601 tttgccgggc tgattctccg gcaccggcag gtaagcaacg ctgggcggca cgaacgggcc
     9661 gaagttggct ccgctgaaga gagcgctgaa tatgtcaatc accgcggtca tgcagtaccc
     9721 cttgtggctg ccgtgcaggc gatcaccccc cagagtgacc attccccctc cgtgccgaag
     9781 aataccgggg tcatcggaag gatttccctc ggcgtcctga acgtatccca acggtacctt
     9841 ctcgcccttc ttttccgaaa cggcgagctt tccgcgcgca ataggcgtag ttgcgaagtc
     9901 tgccacgaag ggcggctcct caagcgcggg aactgctact gcaagcgggt tggtgcccag
     9961 gaggcggctc actgagaagg tgggtaccac aagcgggttg gcgttggtga cagcgatgcc
    10021 taccatatca tgttcgagag ccatcattgc gtagtaaccg gctatgccga agtggtttga
    10081 gttgcgggct gccacccagc ccgtgccggt ggcagccgcc ttctcaatgg ccagatgcat
    10141 cgacctgact cctgcaacca tgccgaagca cctgtcgccg tcgacaacag ccgtggaggg
    10201 ggtttcatgt accaccttca cgtcagggct gacattcacc cgtccgctct tccacagctc
    10261 gtagtactcg cggaccctta tcatgccgtg cgatgagtgg ccgcgcagct ccgccgccac
    10321 cagtacggcg gcaacggcag cagcatcatc agagttgcac cccacccggg tgaaaacttc
    10381 ggtggtgaaa tcaaggagat atttgtggtc gtacataatc cttcattgct tacttcctca
    10441 ccttcaccgg atacggcggt ctgacgggct cggccggcgg gttcttctcc agctccttaa
    10501 gagccacctc gatggctttc tcaagctgag gatccctgcc ttcaagcaca tcggcaggcc
    10561 actgctcaac ttcaatgtca ggggccacac ctacattctc aaccacaaaa ccgtcttcgg
    10621 tccagatagc caggttgggt gctgtcacca ttccgccatc catcagttcg gggaagccca
    10681 gtgtccctac cagtccgccc caggtacgct tgcctaccag ggtaccgaca ccaaacttgc
    10741 ggaacatcca gggaagcata tcaccgcctg aacctgccgt ctcatcaatg atcatgacct
    10801 tcggcccctg aattgaagca cttggtgtct tgagatcatt gccgtaacgc atgttccagt
    10861 acgactgtaa cgggcgttga agcagatcaa tgtagtagtc ggcaatcatc cccccgccat
    10921 tgaaccgttc gtcaatgatg acagccttcc tgttggcctg
//
LOCUS       DAOPXU010000093        10799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_7006_length_10799_cov_10.308917, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000093 DAOPXU010000000
VERSION     DAOPXU010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10799
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7006_length_10799_cov_10.308917"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="PKK03_09320"
     CDS             <1..1359
                     /locus_tag="PKK03_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HNX84640.1"
                     /translation="MRTNIMMLAIFVAALILWITEAIPNYLTSLIIIISLILTKVLPE
                     KEALAQLGHPVMWLNIMSFVLASMLVATGVAKRFALWFIIKFGKNASSIFISFILINL
                     VLSAFISATTAKAAILLPIFMVIASIYGAHSGTRNNFGRSIVLQNLLNINIGAGAFVT
                     GSGANLLAAALIGGAIGGKVFFGDWMMAMFPIMVGLMFIGYLVAMKIFFPLAPGERLP
                     QIEGGMDRLREELKKLGKIDIQEIKSIILFVLILGFWATDRIHGISATSVAFVGAVIA
                     LLPRVGIVKWNDVDVPWHLMLFSAGAYTLGSGFDYTDLPSITVNAIFDHMGIGQETPF
                     WLLYILLTGVMVFSALLFQSKTMRAMIFIPIAIGVANRFGFSVISLALPVAFMIEHVY
                     VLPFNSKPAALLYETDQYSISDAFKFGITMMIIAWLLIIIAGETWFRFLGITPNGVFG
                     LF"
     gene            1371..2834
                     /locus_tag="PKK03_09325"
     CDS             1371..2834
                     /locus_tag="PKK03_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84641.1"
                     /translation="MIEKNVKIHDKFSLELKFGYSTRRKLKENLFEVNTWIFIPNSLD
                     INRQTYDKSDFYKDLKSNIRLITPEFLLRDIADRTKSPFIHLEASFNALAATPTRGNA
                     REYEHQIKMFNSILKSSLRDHRVHTIRNIETEDRDYLISNYLQSSREILSNYRSLRHI
                     IAVPTVSREQFSLYLLGDEFMSNLIEQHTFLLADEIRKRYPELYSQTGPMLTELIRGE
                     VEYRKSKGFPVAENRGREEIRELIYRRGVLKKFFESQLFLATRKKKDGFIAEQLIYSI
                     AAGFAMIFATGIAFAFQQKYGNFTIPLFVALVISYMLKDRIKELTRYYFAGKLKKIFF
                     DHKTRISVTPDKSVGWCKESFDFVSAEKVPADILGIRGKSLALEVQSVKGWEKVVLYR
                     KLMKLDRPSLDGIFTHYTITGVNDIIRFNVSNFVHNMDNPEIPFRTLAGDDYEEISGQ
                     KVYYLTFVIDVRFDGENQVTLYRVTFNRSGILKVDSL"
     gene            2893..3420
                     /locus_tag="PKK03_09330"
     CDS             2893..3420
                     /locus_tag="PKK03_09330"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNX84642.1"
                     /translation="MKLARRNVASIVIASLLIITAIAIASLTVGRDIYYGSRDQGLLS
                     FAIVNACGYLFFLFMPVEIAFVYYLNGDISILALNAVAIGTALFSQSIDYAIGVLVSD
                     KIIDNLIGRRRYKRAEAKIEKYGNLTIFIFNALPLSSPVISLAAGMLRHNIRGTVIFT
                     VLGLLVKYAALTLIF"
     gene            complement(3450..4127)
                     /locus_tag="PKK03_09335"
     CDS             complement(3450..4127)
                     /locus_tag="PKK03_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84643.1"
                     /translation="MKDRISTLVLFAAALLLITTATGCEKDDNKPASDKLEGLIYYYS
                     DEVNKQIRVIDLNAVSPFRRTYTLSEEVTMDVIAQDREELVIKVYRPASTSPMADKKG
                     MLLKSKNANGEWLFIGNYPWVGLTGIADGETWFEASEYAEGATGFRFHEKEVINGETI
                     YAIESTKYPGWYFSHNGHPIQGANLLCLEEFPDVDKAPGFRIYKPGVQVLEGDPSVPH
                     DVEIGGE"
     gene            complement(4374..5438)
                     /locus_tag="PKK03_09340"
     CDS             complement(4374..5438)
                     /locus_tag="PKK03_09340"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNX84644.1"
                     /translation="MKLLKILLLLSVLPFAGCDKNEDVRPDDFMYFPSGNEWDTRSVS
                     DMGWNESAVQPLKDFLVQKNTRSFMILVNGRIVMEEYFNGHTPSTTWQWNSAGKTLVS
                     AVTGIAQQEGLLDIDNRVSDYLGAGWTSEPTEKENLITLKHLLTMTSGVNDGSNLVIR
                     ANLTYLADAGTRWSYHNVFQLLMDAVADAGSQEFETFFSSRLKNKIGMDGYWNNGIIF
                     KIYHSNTRSMARFGLLALNKGKWENEQILEESFFNAGTSSSQDMNPSYGYLWWLNGKT
                     SYMVPGSQNVYLGSLVPKAPSDMFAAMGAEDQRIYVIPGKKMVVVRMGEASDPQNPSF
                     ALSGFDNALWEKIGAVIGTK"
     gene            complement(5515..6048)
                     /locus_tag="PKK03_09345"
     CDS             complement(5515..6048)
                     /locus_tag="PKK03_09345"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNX84645.1"
                     /translation="METTKWVIDPAHSEIQFKVKHMMITTVTGSFKEFKSEVETKGDD
                     FTTAKISFEANTASVFTNAEQRDAHLRSADFFDADNFPVMSFVSSRLEKIDDESWQLN
                     GDLTIRGVTNPVRLDVEFGGVGKDPWGNTKAGFSLNGKINRKDWGLNWNAALEAGGVL
                     VSDDVRIFCEIQYSKQA"
     gene            complement(6154..7068)
                     /locus_tag="PKK03_09350"
     CDS             complement(6154..7068)
                     /locus_tag="PKK03_09350"
                     /inference="COORDINATES: protein motif:HMM:NF004846.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNX84646.1"
                     /translation="MNKWTTGRIGELNGKRIVITGSSHGIGFEAAGILASKGAEVVLA
                     VRNREKGEKAAVKIRPLNGSKPVTVMNLDLADLESVRKFAEEYATRFDRLDVLINNAG
                     VMIPPYKMTKDGFELQFGTNHLGHFALTGHLLPVVMNTPLSRIVTISSIAARRAKIHF
                     DNLDGSKGYNPMTFYRQSKLANLLFAMELQNRLEVAGSTTISVACHPGISVTNLLSRG
                     TGRETGRVMKALMGIVAQPASMGALPTLYAATHPDLRGGEFIGPDGPGNTKGYPVLTN
                     DAARLYKPDLAAKLWEVSETLTGVRYSI"
     gene            complement(7135..7812)
                     /locus_tag="PKK03_09355"
     CDS             complement(7135..7812)
                     /locus_tag="PKK03_09355"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HNX84647.1"
                     /translation="MTMIRKATVGLIAMLFSVIAAGQKTDYGIWYEASASKDIWKGLR
                     ADLEASVRTNENAGNIEKFYLEPGLRYKFNDYFNAGVYYRFIEQKEKDDLYHARHRWF
                     FQMKGTAPSVARFTFSVRYRIQQQFRTYYKDPEDREPQWYQRVRLELDYDVRGIPLKP
                     YINTEMHLQLSDPNDIVADKWRSMVGIEYTFNKRHTFGLEYIYNDSRVTKPPFENFLG
                     VTYSVKL"
     gene            complement(7799..8500)
                     /locus_tag="PKK03_09360"
     CDS             complement(7799..8500)
                     /locus_tag="PKK03_09360"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HNX84648.1"
                     /translation="MNITGMTLPDSSVVMAGQGMFSFAGLPELLLRFCINLVVMLVLV
                     RWLYYSTTRRKDYLFTYILISSVVFLLCYLLESVSLQIGFALGLFAIFGIIRYRTNPM
                     PIKEMTYLFLVIGISVINALTAGTSMIEIIFANLAVILITLGLEKVWLLRHISSKSIV
                     YEKINLIVPEKHDEMIADLQQRTGIKEIKKVEIGAINFLKDTCRMIIYYEDRGRNVNL
                     ADQNGFIKPDDNDDD"
     gene            complement(8469..9257)
                     /locus_tag="PKK03_09365"
     CDS             complement(8469..9257)
                     /locus_tag="PKK03_09365"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HNX84649.1"
                     /translation="MSELSEIIEILEGFEPVDLDGISAVRYMNRVDTKYLFSAGRLPD
                     LLRRGQQHYSALEIESTREFSYKTIYFDTPDLLFYTQHITGKLNRTKVRIRTYESNGL
                     TFLEVKQKSNKGRTRKSRLEKESGEMNHIEQSREFLEELVSPEAVALKPVINTSFTRI
                     TLVSLERSERITIDYNLAWNNLRGDSVRMPFLAIAEIKSDKSTSMSPFFLMLKDMGVR
                     RTGFSKYAVGMALVNRTARQNNLKPKFLLINRIRDEYYRNDAAR"
     gene            complement(9367..10797)
                     /locus_tag="PKK03_09370"
     CDS             complement(9367..10797)
                     /locus_tag="PKK03_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005808345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNX84650.1"
                     /translation="MIADDLGYGDTGPYGQSKIETPNIDKLAAEGLRFTHFYAGSPVC
                     APSRCVLLTGMHTGHSQVRGNDEWAERGDVWSYRAMIADSTLEGQKPMADSTVTIASL
                     LQKNGYITGAVGKWGLGAPHTGGSPVRQGFDFFFGYNCQRIAHTYYPTHLWRNDHRVW
                     LRNDTLAPHTGFDPEADPLDPASYEKFSLADYAPDLMFREMLGFIDSCSGRPFFLYWA
                     TPIPHLALQAPAGWVDRYHEKFGDEPPYNGNEGYFPARYPHAAYAAMISYLDDQIGSL
                     IAHLKEKGLYENTIIFFTSDNGPAWSAGTDPEWFASASPFRGTEGYGKGSLNEGGIRV
                     PLIASWPGVTEPGGVTGHVSAFQDFLPTISEITGSEIPGDADGISFLTVLRGGKQREH
                     DYLYWEFPEYGGQQAVLIGKYKALRKGMHNGNDAFALYDLENDPQETVDISADHPEIM
                     KRVGEIVRKEHNASPNPRWRYRLLGE"
BASE COUNT         2633 a         2779 c         2502 g         2885 t
ORIGIN      
        1 atgcggacga atatcatgat gctggcgatc tttgttgccg ctcttattct gtggatcacc
       61 gaggccattc ccaattacct gacctcattg ataatcataa taagcctcat actgaccaaa
      121 gtgcttcctg aaaaagaggc tctagcacag cttggacacc cggtcatgtg gctgaatatc
      181 atgtcgtttg tactggccag catgctcgta gccaccggag tggccaaacg gtttgccctg
      241 tggttcatta ttaaattcgg gaagaatgca tcatcaatct ttatcagttt catccttatc
      301 aacctggtgc tgtctgcatt catttcggcg acaactgcaa aagctgccat acttcttccc
      361 atattcatgg ttatagcctc aatttacgga gcacactcag gtacgaggaa taatttcggg
      421 cgaagcattg tccttcagaa cctgctgaat ataaacatag gagccggagc ctttgtgaca
      481 ggttcggggg ccaaccttct ggctgcagct cttatcggag gtgccatcgg cggcaaagtg
      541 ttctttggcg actggatgat ggctatgttc ccgataatgg taggtctcat gtttatcgga
      601 taccttgtag ccatgaagat attctttcct cttgccccgg gcgagaggct cccccagatt
      661 gaaggcggaa tggacaggct cagggaagag ctgaaaaaac ttgggaaaat tgacatccag
      721 gagataaagt caattattct tttcgttctg atactgggat tctgggccac tgaccgtata
      781 cacggaataa gcgccacctc cgtggctttc gtgggagctg tcatagccct cctgcccagg
      841 gtgggaattg taaaatggaa tgatgtggat gtgccatggc acctgatgct tttctcggca
      901 ggtgcatata ctcttggctc gggcttcgac tacactgacc tgccatcgat tacagtcaat
      961 gcaatcttcg accatatggg cataggtcag gaaacgccct tctggctcct ttatatattg
     1021 cttacaggag tcatggtctt cagcgcgctg ctgttccagt cgaaaacaat gagagcaatg
     1081 atatttattc ctattgcaat tggcgttgcc aacaggttcg gcttcagcgt catcagcctt
     1141 gcactgccgg tagcgttcat gattgaacat gtctatgttc ttccctttaa cagcaaacct
     1201 gcagcactgc tttatgagac tgaccagtac agcatctctg acgctttcaa attcggcatt
     1261 acgatgatga tcattgcctg gctgctcatc attattgcag gggagacatg gttcaggttc
     1321 cttggcatca cgccaaacgg agtattcggg ctgttctgac ttgaccggtg atgatcgaaa
     1381 agaacgtcaa gatacatgac aaattctccc tcgaacttaa attcgggtac tcaaccagga
     1441 gaaaactgaa ggagaacctt tttgaagtca atacctggat attcattccc aacagcctgg
     1501 atatcaacag gcaaacttac gataaatcag atttctacaa ggatctgaag tcgaacatca
     1561 ggctcatcac ccctgagttt cttttacgtg acatagctga ccggacaaag tcgccgttca
     1621 ttcatcttga ggcttcattc aacgcactgg cagccactcc tacccgtggc aatgcccggg
     1681 agtatgagca ccagattaag atgttcaact caatcctcaa gtcatccctt cgcgatcacc
     1741 gcgtacacac catacgtaac attgagacgg aggatcgtga ctatcttatt tcgaattatc
     1801 ttcagagctc acgagaaatc ctctccaatt acaggagcct gaggcatata attgcagtgc
     1861 ccacagtgag cagggaacag ttctctctat acctcctcgg tgatgaattc atgagtaacc
     1921 tcatcgagca gcataccttt ctgctggccg atgagatcag gaagcggtat ccggaactgt
     1981 acagccagac cggtccgatg cttacagaac tcatccgggg ggaggttgaa taccggaaat
     2041 caaagggctt tcccgttgct gagaaccggg gcagggagga gatcagggag ctgatctaca
     2101 ggcggggcgt tctgaagaag ttcttcgaga gccagctgtt cctggccaca agaaagaaaa
     2161 aggacggatt tattgctgaa cagctcattt acagcattgc cgctggtttt gccatgatct
     2221 ttgccacggg gatagccttt gcgttccagc agaagtacgg gaacttcacc attccgctat
     2281 ttgttgccct tgtcatcagc tacatgctca aggaccggat caaggagctt accaggtact
     2341 acttcgcagg gaagctgaag aagattttct ttgatcataa gacaagaatc tccgtcaccc
     2401 cggataaatc agtcggctgg tgcaaggaga gctttgattt tgtctcagct gaaaaggtgc
     2461 ccgctgatat cctcggcatc cgcggcaaat cacttgctct tgaagtccag agtgtcaaag
     2521 gctgggaaaa ggtggtgctt tacaggaagc tgatgaagct cgacagacca tcgcttgatg
     2581 gcatttttac tcattataca ataaccggtg tcaatgacat cataaggttc aacgtctcaa
     2641 actttgtgca caacatggac aacccggaga ttccgttcag gactcttgcc ggggacgatt
     2701 atgaggagat cagcggtcag aaggtctatt acctcacatt tgtcattgat gtcaggtttg
     2761 acggcgagaa ccaggtcacg ctgtacaggg tcactttcaa caggtcaggc atcctgaagg
     2821 tagattccct gtaaccaaat ttttctctct attgcaaaat ccttacattt atggcgatcc
     2881 taaaagccgc gaatgaaact tgccagacgt aacgtcgcca gcattgtaat tgcctccctc
     2941 ctgattatta ctgcaattgc aatcgcatcc cttacagtgg ggcgcgatat ttactacgga
     3001 tcacgtgacc agggcctgct ctcatttgct attgtaaatg catgcggcta cctgtttttt
     3061 ctctttatgc cggttgagat agcgttcgtt tactacctca atggcgatat aagtatactg
     3121 gccttgaatg cagtagctat tggcacagct ctcttttccc agtcaataga ttatgcaatt
     3181 ggagtgcttg tcagcgacaa aatcattgac aatctgatcg gccggcgccg ctataaaaga
     3241 gctgaggcaa aaattgagaa atacggcaat cttacaatct tcattttcaa cgctttgcct
     3301 ctctcctccc cggttatttc actcgcggca gggatgttac gtcacaatat ccgtggcaca
     3361 gtgatcttta ctgttcttgg gctgctcgtc aaatatgccg ctcttacact gatcttctga
     3421 tgaaaggatc gctgtggaaa tgacgaagac tattctccgc caatctcaac atcatgcggg
     3481 acactgggat caccctcaag aacctgtact cccggtttat agattctgaa tccgggggcc
     3541 ttgtcgacat cggggaactc ctcaaggcaa agcaggttag cgccctggat gggatggcca
     3601 ttgtgggaga agtaccagcc aggatacttt gtgctttcaa tcgcataaat ggtctcgccg
     3661 tttatcacct ctttctcatg gaaccggaag ccggtggctc cctcagcata ttcacttgct
     3721 tcaaaccagg tttcaccatc ggctattcct gtcagtccga cccatggata gttgccaata
     3781 aacagccact cgccatttgc gtttttggat tttaacagca ttcctttctt atcggccatc
     3841 gggctggtag atgcaggtcg gtaaaccttt atcacaagct cttccctgtc ctgtgcgatt
     3901 acatccatgg taacctcctc tgagagggta taagttcgcc tgaatggaga gacagcattt
     3961 aaatctatta ctctgatctg cttattcact tcatcactgt aataatagat taatccttca
     4021 agtttgtctg atgcaggttt gttgtcatct ttttcacaac cggttgcagt tgtaattaat
     4081 aacagggctg ctgcaaacag aactaaagtg gagatacgat ctttcatagt cattactttt
     4141 tacggatttt cggaattggt tcaagtattt aacacaaaca tgccggaaac aggacaaact
     4201 caataaaata caggcaatat caattacttg tcaagaaacc accaagtgta tcaaaaagtt
     4261 cagagacaga taaattttac catccagggc gtagaattgg ccctgttatg caggtaggta
     4321 atgcaatcag tggagataat tcagctgaca agccgctgag gatcaacagg accctacttg
     4381 gtgcctatga ccgcacctat tttttcccag agagcattgt caaatcccga aagggcaaag
     4441 ctcgggttct gaggatcaga ggcctccccc attcttacta ctaccatctt tttgccgggg
     4501 ataacataaa tacgctgatc ttccgctccc atggcggcaa acatatctga gggagccttc
     4561 gggacaaggc tccccaggta aacattctgc gatccgggaa ccatgtaact ggttttgccg
     4621 ttcagccacc acaggtaacc gtaggacgga ttcatgtcct gggaggagct tgtgcccgca
     4681 ttgaaaaatg actcttccaa tatctgctcg ttctcccatt tccctttatt gagcgccagg
     4741 aggccaaacc tggccatgct gcgggtattg ctgtgataaa tcttaaatat aatcccattg
     4801 ttccagtatc cgtccatccc gatcttattc tttaacctgg aactgaaaaa ggtttcgaac
     4861 tcctggctgc cggcatccgc aaccgcatcc atcaacagtt gaaagacatt atggtatgac
     4921 catcttgttc cggcatcggc aagatatgtg aggttcgccc taatcacaag attggatccg
     4981 tcgtttacgc cggaggtcat tgtgagcaga tgcttaagtg taatcaggtt ctctttctca
     5041 gttggttcac tcgtccaccc tgcgcccaga taatcagata ccctgttgtc aatatccagc
     5101 aatccttcct gctgtgctat gcctgttact gcagagacca gtgtctttcc ggcgctgttc
     5161 cactgccatg tggtggatgg ggtatgtccg ttaaagtact cctccatgac aattctgccg
     5221 ttgaccagta tcataaagga tctcgtattc ttctgaacca ggaaatcctt cagaggctga
     5281 actgcacttt cattccaacc catgtctgac acagaccttg tatcccattc attgcctgaa
     5341 gggaaataca taaagtcatc aggtcggaca tcctcgttct tgtcgcaccc ggcaaacgga
     5401 agtaccgaca gcagcagaag gatcttaagg agcttcatat atttaaagac ttgtcccgca
     5461 aaatggtttc tcatttatac gaaacaagtc ttaaattgtt ttatctgcaa caggtcaggc
     5521 ctgctttgag tactggatct cgcagaatat gcgaacatca tcacttacaa ggactccgcc
     5581 tgcctccagg gctgcattcc agtttagccc ccagtccttg cgattgatct tgccattgag
     5641 gctgaatccg gccttggtgt tgccccacgg gtccttgcca acacctccga attcaacatc
     5701 aagcctgaca ggatttgtga ctcccctgat ggtaaggtcc ccattaagct gccagctctc
     5761 gtcatcgatc ttctccaggc gtgatgaaac aaaactcatg acgggaaaat tgtcggcatc
     5821 gaagaaatct gcactccgga gatgtgcatc tctctgctcc gcattggtga agactgatgc
     5881 ggtattggct tcgaaactga tttttgccgt tgtgaaatcg tcaccctttg tctcaacctc
     5941 agatttgaat tccttgaaac tgccggtcac ggtggtgatc atcatgtgtt tcaccttgaa
     6001 ctgaatttcg ctgtgtgcgg gatctatcac ccattttgta gtctccatga taatattctc
     6061 tgtttttgtt gaacgtttct gtttctgctc ttaaaacaac aagcagccag gattgttcaa
     6121 cagaaggtgc acaaaatacc ggagactgaa ggattaaatt gagtaacgca caccggtcag
     6181 agtctctgac acctcccaca gctttgccgc gagatcaggt ttgtagagcc tggcagcatc
     6241 atttgtaagc acgggataac cctttgtgtt tcccgggcca tccggaccga tgaactcgcc
     6301 tccgcgaagg tcaggatggg tagcggcata cagggttggc aatgctccca tcgatgcggg
     6361 ctgggccact atccccatga gcgccttcat caccctgccg gtctctcttc ctgtcccgcg
     6421 ggagaggaga tttgtgacac ttatacccgg atggcaggca acactgatgg ttgttgaccc
     6481 ggcaacctca agcctgttct gcagctccat ggcgaacagc agattggcca gcttgctctg
     6541 gcggtaaaat gtcatgggat tatacccctt tgagccgtca agattgtcaa aatgaatctt
     6601 agccctcctg gcggctatac tgctgatggt gacgatacgc gacaacggcg tgttcatcac
     6661 caccggaagc agatgaccgg taagggcgaa atgaccgaga tggtttgtgc cgaactgaag
     6721 ttcaaaccca tccttcgtca ttttataagg cggaatcatc actccggcat tgttgatcag
     6781 cacatcaagc ctgtcaaaac gggttgcata ctcttcagca aatttcctga ccgactcaag
     6841 atcggccagg tcaaggttca tcaccgtcac aggttttgat ccgttcaaag gcctgatctt
     6901 tacagcagcc ttttccccct tctccctgtt ccttactgca agaaccacct cagctccctt
     6961 tgacgccaga attccggccg cttcaaaacc tatcccgtgt gatgatccgg taattactat
     7021 cctctttccg ttaagttctc cgattctccc ggtagtccat ttattcatgt ccgttcttca
     7081 atatataaaa aacagcctgg ggctgaaaat ctgccgcaaa tatacagaat gaaactaaag
     7141 tttcactgaa taggtaacgc caagaaagtt ctcgaacggc ggctttgtta ccctgctgtc
     7201 gttgtaaata tactcgagtc cgaaagtatg cctcttgttg aaagtgtatt ctatccctac
     7261 catcgatctc cacttgtcag caacaatatc gttgggatca gaaagctgca gatgcatctc
     7321 tgtatttata tagggcttta acgggatacc cctgacatca tagtcaagtt cgagccttac
     7381 cctttgatac cattgcggtt ccctgtcctc cggatcttta tagtaagtcc tgaactgctg
     7441 ctggatacga tatctgactg aaaaggtgaa gcgggctacc gagggagccg tgcccttcat
     7501 ctgaaaaaac caccggtgac gtgcgtgata caggtcgtcc ttctccttct gctcgatgaa
     7561 cctgtaataa acgcctgcat tgaagtagtc attgaacttg tacctcaatc cgggctccag
     7621 gtaaaacttt tctatattcc cggcattctc attggtcctg acactggctt ccagatcagc
     7681 cctgagtccc ttccagatat cctttgaagc actcgcctcg taccatatac cgtaatcagt
     7741 cttctgcccc gccgcaatca cggaaaacag cattgcaata agcccgactg tggcctttct
     7801 aatcatcgtc attgtcatcc ggtttaatga atccgttctg gtcagccagg tttacattgc
     7861 gtcctctgtc ctcgtaatag attatcatcc tgcaggtgtc cttcagaaaa ttaatcgctc
     7921 ctatctcaac cttctttatc tcctttatcc ctgttctctg ctgcaggtca gcaatcattt
     7981 cgtcatgctt ctccggcact atcaggttga tcttttcata gacgatcgat ttggatgaga
     8041 tatgcctcag caaccacacc ttctccagtc ccagggtgat tagtatcaca gccaggttgg
     8101 cgaagatgat ctcaatcatg ctggtgcctg cagtaagggc attgatcact gaaataccta
     8161 taaccaggaa gaggtaggtc atctccttga tgggcatcgg gtttgtccgg tacctgatta
     8221 tgccgaagat tgcgaacagg ccgagtgcaa accctatctg aaggctgacg ctctcaagca
     8281 ggtaacagag caggaaaacc acgctggaaa tgagaatgta cgtaaacagg taatcctttc
     8341 tcctggtagt gctgtaataa agccagcgca ccagaaccag catcaccacg aggttgatgc
     8401 agaaacggag caataactcc ggtaatcctg cgaagctgaa cattccctgt ccggccatga
     8461 ccacactgct atcgggcagc gtcattcctg taatattcat ccctgatcct gttaattaat
     8521 aaaaacttcg gttttagatt attttgcctg gctgtcctgt ttaccagagc catgccaact
     8581 gcatacttgc tgaatcctgt cctgcgcacc cccatatcct taagcatgag aaagaaggga
     8641 gacattgagg ttgatttgtc actcttgatc tcggcaattg cgaggaaggg cattcttacg
     8701 ctgtcacccc tgaggttgtt ccatgccagg ttatagtcaa tagttattct ctcggatctc
     8761 tccagactca caagggtgat gcgggtaaaa gaggtgttta tgacaggctt cagtgccact
     8821 gcttccgggg agacaagttc ctcaaggaac tccctcgact gctcaatgtg attcatctcg
     8881 ccggactcct tttcaagccg cgatttgcgt gtgcggccct tgttcgactt ctgcttcacc
     8941 tccaggaagg tcagtccgtt cgactcgtac gtcctgatcc tgaccttggt cctgttgagc
     9001 ttgccggtga tgtgctgggt atagaacagc aggtcgggag tgtcaaaata tattgttttg
     9061 taagagaatt cccgcgtgct ttcaatctcg agggcgctgt agtgctgctg cccccttcgg
     9121 agaagatcag gaagcctgcc tgcagaaaag agatacttgg tatcaacgcg gttcatgtac
     9181 ctcacggcgg agatgccatc gaggtccacc ggctcaaatc cttcaagtat ttcaattatt
     9241 tctgacaatt cactcattcc ggaacccgcg caaagatagc agacttcgcg taggaagaaa
     9301 gcctttttat catgccggtg gatttaaatt ataacgtccc ggaagcgtgt aatatttcac
     9361 cctgggtcac tctcccagca gcctgtacct ccatcgcggg ttcggtgagg cattgtgctc
     9421 cttacggact atctccccga cccgcttcat gatttcagga tggtctgccg aaatgtcaac
     9481 agtctcctgc ggatcattct caagatcata aagggcaaaa gcatcattgc cattgtgcat
     9541 ccccttgcgg agcgccttgt actttccgat gagcacagcc tgttgccctc cgtactcggg
     9601 aaactcccag tagagatagt catgctctct ctgtttccct ccgcggagca ccgtaaggaa
     9661 actgatgccg tcagcatctc cgggtatctc tgagccggtt atctcgctaa tcgtcggaag
     9721 gaagtcctgg aaggcggaga catggccggt gacacctccc ggttcagtca ctccgggcca
     9781 tgaggcgatc agcggaaccc tgataccgcc ctcgttgagt gaacctttgc cataaccctc
     9841 tgttcccctg aagggggaag cactggcaaa ccattcagga tcagtacctg cgctccatgc
     9901 cggaccgttg tcactggtaa agaaaattat ggtattctca taaaggccct tctctttaag
     9961 gtgagcaatg aggctgccaa tctggtcatc aaggtatgag atcattgcag cgtaggcggc
    10021 atggggatat cttgccggaa aatagccttc attgccattg tatggtggtt catcaccgaa
    10081 cttttcatga tacctgtcta cccatccggc cggcgcctgc agcgccaggt gaggtatggg
    10141 agttgcccag taaaggaaaa acggtctacc ggaacaggag tcaatgaacc cgagcatctc
    10201 cctgaacatc aggtccggcg cataatcggc gaggctgaat ttctcatagg aggccggatc
    10261 aagcgggtca gcctccgggt caaaccccgt atgcggagca agagtgtcgt tcctcagcca
    10321 tacccggtgg tcatttcgcc agaggtgtgt cggatagtaa gtgtgggcaa tcctctggca
    10381 gttgtatccg aagaagaagt cgaatccctg cctgacaggg cttccgccgg tgtgaggtgc
    10441 accaaggccc catttgccga cagccccggt tatatatccg tttttttgca gcagtgaggc
    10501 gatggtgacg gtgctgtctg ccatgggttt ctgcccctcc agggtggaat cggctatcat
    10561 cgccctgtag ctccagacat ctcccctttc tgcccactcg tcgtttcccc tgacctgcga
    10621 gtggccggtg tgcatacctg tcaggagaac acatcgtgaa ggcgcacata ccggtgaccc
    10681 tgcatagaaa tgagtgaacc gcaatccctc agccgccagc ttatcaatat tgggagtctc
    10741 aattttcgac tgcccgtaag ggccggtgtc accgtatccc agatcgtcgg caataatta
//
LOCUS       DAOPXU010000094        10730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_7085_length_10730_cov_16.265855, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000094 DAOPXU010000000
VERSION     DAOPXU010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10730
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7085_length_10730_cov_16.265855"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..839)
                     /locus_tag="PKK03_09375"
     CDS             complement(240..839)
                     /locus_tag="PKK03_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNX84651.1"
                     /translation="MEGFVKFNCYWSQSGPVITDEQYEIINHWREILFNLDLVGAFEN
                     GVGFGNISIRIGKSSQFIITGSSTGDIPELEPGHYVKVSSFNIDDNAVMCSGPLKASS
                     ESLTHAAIYTADPGTNAIIHVHSTRLWEELMNKVPTTDPAVEYGTPAMAREMIRIFSE
                     PEVFEKRIIVMAGDRGGLITFGYDLDEAGGVLFDYINRQ"
     gene            complement(845..1918)
                     /gene="mtnA"
                     /locus_tag="PKK03_09380"
     CDS             complement(845..1918)
                     /gene="mtnA"
                     /locus_tag="PKK03_09380"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNX84652.1"
                     /translation="MKVGERYYRSIWHDKADRGSVHVIDQRRLPFAFEVMTLRSVSDV
                     YDAIREMAVRGAPLIGAAAAWGVYLSFLEHSSGKKNETGNVYNAVISDAQRLKSARPT
                     AVNLAWAVDYMTGVMKFFPDADALCRAAEGLCDEEAERSRLIGVNGLRVIEAASSMKG
                     GETVNILTHCNAGWLACVDYGTALAPVYMAHDRGLKVHVWVDETRPRNQGSRLTAWEL
                     LNHGVPCTLITDNAGGHLMQNGMVDMVIVGCDRATASGDVANKTGTYLKALAAFDNGI
                     PFYSALPVSTFDLSVDRPGQIEIEERDAGEVSTIDGIRIAPDGVNVSNYGFDVTPARL
                     ITGLITDRGICRADKESVLKTVS"
     gene            2084..2668
                     /locus_tag="PKK03_09385"
     CDS             2084..2668
                     /locus_tag="PKK03_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX84653.1"
                     /translation="MVDKEEYRARIIRTASRIFSHYGFRKTTMDEISRALKKGKSSIY
                     YYFPGKEEIFEAVVLYEANQLRRRLTAAIKEVDSPPDKLRNYIYVRMREFAKLSNYYN
                     AVFDKNLDHYEFIEKIRARYDREELAIIRLLVYVGNNRGTFAVEDSEYTAMAIQTMLK
                     GMEVPLFWRRRETDINYRLEAILTLIFNGIMKKQ"
     gene            complement(2772..3614)
                     /gene="lgt"
                     /locus_tag="PKK03_09390"
     CDS             complement(2772..3614)
                     /gene="lgt"
                     /locus_tag="PKK03_09390"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNX84654.1"
                     /translation="MELQIIPWNVNPEIFRIGNFAVRWYGLLFASGFVFGYYIMRKIF
                     RNEGLTDATLDRLTVYMAIGTIVGARLGHCFFYEPEYYLARPLEILKVWHGGLASHGA
                     AIGILLALWLFVRKEKKPYLWIIDRVVIVVALAGTMIRLGNLMNSEIYGVETTLPWGF
                     VFLRNGETAPKHPTQIYEALAYLITFGVLMRIYWKNLGKQKPGLLFGLFLIFVFGFRF
                     LVEYIKEDQVGFEAGMKLNMGQWLSIPLVLAGIALLVWVFTRKPEPVPVTEQWSPKKG
                     VPKK"
     gene            3742..5544
                     /locus_tag="PKK03_09395"
     CDS             3742..5544
                     /locus_tag="PKK03_09395"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HNX84655.1"
                     /translation="MDRGRYITGSLGLTVLATLFVLSCAKISAPTGGPKDLDPPVILK
                     SIPENGSVLFTGKSFTVIFDEYVVLDRITEKFMVSPPLATKPDVKLRGKSLVVNWEDD
                     LADSTTYTFYFQDAIRDNNENNPINNYQYVFSTGPVLDSLSLTGNVFNASDLEIVEDV
                     TVIMYSNLSDTAPRTILPAYISRPDPSGGFMISNIRPGNYRLFALKDLNGNTRYDLAD
                     EIFAFGDSVIVITPDNYFGLVPDTVTYRPANATETTKPDKFVYGLHRLYAFQEPPKQQ
                     YLTFTERKSSWGISFGMALPSDSGQVSVTLPEADSSSWYMEHNVARDTFLIWVTNPDV
                     YSRDLVDAILTYPFTDSTGAIIAKTDTISFRYMKPAAPRGGAGRTPGLSITTNISGKI
                     RPGTVPLFRSATPLADPDTSLIRLTRTVDSVQVVLPYEFVRDTVSSRLLFLKAQLEPG
                     ATYSLLCRKGAFSDIFGVASDSAMYRISVATEEDYGSLRIKIAGYEGRVIIQLTADAK
                     DKVIREESLTSPGEVSFPLLEKGKYRLKAIYDLDSDGKWTTGDFNAGRLPEPVTYYPA
                     ELEVKINWALVQDWDIGEMYVKDVSLRNKPATRR"
     gene            5549..6979
                     /locus_tag="PKK03_09400"
     CDS             5549..6979
                     /locus_tag="PKK03_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HNX84656.1"
                     /translation="MSTRRKFIRNSMVAGAAFSAGIPSFVSASNRKRPSVRPAARVRK
                     FRSKAVEETIKEIESFLGSDNELAWLFSNCFPNTLDTTVNFSEKEGRPDTFVITGDIN
                     AMWLRDSSAQVWPYVSLAPGDPQLKDLIAGVINRQVKCIHIDPYANAFNDGPGQSEWM
                     NDLTDMKPELHERKWEIDSLCYSVRLAHGYWKATGDTSVFDSSWSSAMRLIIKTFREQ
                     QRKDNPGPYKFQRVTGWQTDTVAGAGYGNPVNPVGLIVSIFRPSDDATIWPFLVPSNY
                     FAVSALRQIGEIAADITRDETLLVDATALADEVDAALAKYARAAHPEHGEIIPYETDG
                     YFNYNFMDDANVPSLLSMPYLGTIDRSDPLYLRTRKFVLSKSNPYYFSGISGHGVGSP
                     HTGLDRIWPIAIIMKALTASNNYDIITSLRMLKNTHAATGFMHESFMKDDSSKFTRKW
                     FAWANTLFGELVLHTYRTQPELLKEV"
     gene            6992..7945
                     /locus_tag="PKK03_09405"
     CDS             6992..7945
                     /locus_tag="PKK03_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNX84657.1"
                     /translation="MKEVVAGVDIGGTNTMIGFVDADGVIHAEGRLSTPDYAFADDFV
                     IALDAKLKEMVAENSGLKLIGIGIGAPNANFNKGTIELAPNLRWKGIIPLVRITEKLT
                     GLKTRITNDANAAALGEMIFGAAKGVKDFIILTLGTGLGSGIVVDGEVVYGHTGFAGE
                     LGHMTVVEGGRVCGCGRRGCLETYASATGLVRTVLSLLSDRRDESLLRELRGSEITSR
                     RIAEAAAAGDIIAQEALDQTARMIALGIANSIIFSSPEVIYLFGGLAKAGDALFVPVR
                     KYADELVQPVFRGTFRVEPSGLPEAQAAILGASALIWKEAR"
     gene            8129..8782
                     /locus_tag="PKK03_09410"
     CDS             8129..8782
                     /locus_tag="PKK03_09410"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HNX84658.1"
                     /translation="MRIIEVRRLKTDREIPPMAEVSARLDELGEGHRIGTVNWQSHTY
                     KPEVRFNIAWGEKEIYIKYYVREAHVKAEKGTTNEMVCEDSCVEFFVSPEDDGIYYNF
                     EFNPIGTTLMGSGHGRHDSARTDPAIVNNIRRLTTMGSEPFPEISGDISWSITVAIPL
                     GTFFRHSVGSLAGKSFRANFYKCGDKLTTPHFVTWNPVGTEKPDYHRPEFFGILKFI"
     gene            8847..10052
                     /locus_tag="PKK03_09415"
     CDS             8847..10052
                     /locus_tag="PKK03_09415"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HNX84659.1"
                     /translation="MIKGNAVIGQSGGPTSVINSSLAGVIDAAKRSGLVSAVYGMNYG
                     IEGFMNEWLFDLSAQPAEIIHGLRHTPSSALGSSRYKLKDDDFPKILSVLQKFDIRYF
                     FLIGGNDTMDTVNRVEAYCRTMGYELTGVGIPKTVDNDLFGTDHTPGFASAAWSNILN
                     VMQAGTLARDMMKVDQFVVYQTIGRDAGWLAAATALARRDEDEAPHLIYTPEHPFIRE
                     KFLADADHCIRRYGWVFVVAGEGIKYEDGTPVSASVVKDKFSNTEFGAMGGASVAITL
                     HRMLSSEFGIRGEFQVPESLIMSDYVRALPADLEEAYRCGIEAVRMAERGESGKMVTI
                     ERKSDDPYEVVYGMVPLGKVAIAARPMPEEYFNAAGNYVSDAFARYMKPLAGAIPEFV
                     RLKKIMVIK"
     gene            10064..>10730
                     /locus_tag="PKK03_09420"
     CDS             10064..>10730
                     /locus_tag="PKK03_09420"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNX84660.1"
                     /translation="MATEFNKVALSINAHPDDAEAWNAGALKLLHDRGYKIVIATMTG
                     GDLGGCNMSMEETARVRYEEAKAAAAVLDAEYYTMGGIDGSLFDSREMRLKVISLMRR
                     VRAGIVFTHLPNDYHPDHRATAAIAEAAAMVASLDPVPVPEKPLEVTPLLYHTSPLTL
                     SDPLGSQLTPPHFFVDVTSAVQTKIEMLGRHVSQLELMKHMHKIDDFFGYVLEGNKNY
                     GKLA"
BASE COUNT         2647 a         2889 c         2758 g         2436 t
ORIGIN      
        1 tgctgctaac catctgagct atgggcccga aataacaggg tgcaaatata atatcaaatt
       61 gaaatatctg caaacctttt acaatgccgg atcccctaag ggaaattttt atcatatgtt
      121 aatcttctac aatgctgaat ggcctgcggc caaaatcaca aatcgcacat ctacaatcct
      181 ttatggcctg cggccaaaat cgcacatcgc aaatcgcaaa tcgcaaatca acagccttcc
      241 tactgtctgt tgatgtaatc aaacagcacc ccccctgcct catccaggtc ataaccgaag
      301 gtgataaggc cgccacggtc accggccatg acaatgatcc tcttctcaaa cacctcaggc
      361 tcactgaaaa tgcgaatcat ctcccgcgcc atcgccggcg tgccatactc aacggccgga
      421 tctgttgtcg ggaccttgtt catcagctct tcccacagtc gcgtcgagtg aacatggatg
      481 attgcattgg tgcccggatc agccgtgtag atggctgcat gggtgagcga ctccgatgag
      541 gccttcagcg gcccgctgca cataaccgcg ttgtcgtcta tattaaatga cgatactttt
      601 acataatgcc cgggctcaag ctcggggata tcaccggtgg atgatccggt tatgataaat
      661 tgagacgact tcccaatgcg tatgcttata ttcccgaacc ctacgccgtt ctcaaatgca
      721 ccaacaagat caagattgaa gaggatctct ctccagtgat tgatgatctc atactgctcg
      781 tcagtgatta ccggacccga ctggctccag tagcagttaa acttgacaaa cccttccata
      841 taacctatga tactgttttt aataccgatt ccttatccgc cctgcaaatt ccgcggtctg
      901 taataagacc ggtgataaga cgcgcaggtg taacgtcaaa cccataattc gaaacattaa
      961 caccatccgg tgcaattctt atgccatcaa tggtacttac ctccccggca tcacgctcct
     1021 cgatctcaat ctgtccggga cggtcaactg acaggtcaaa tgttgatacc ggcagtgccg
     1081 aataaaaagg tatcccgtta tcaaatgccg caagagcctt gagatatgta ccggttttgt
     1141 tggccacatc accggacgcc gtcgccctgt cgcacccgac aatcaccata tcaaccattc
     1201 cgttctgcat caggtgcccg ccggcattgt cggtgatgag ggtgcacggt accccgtgat
     1261 tgagcaattc ccatgccgtg agacggctac cctggttgcg gggacgtgtc tcatcaaccc
     1321 atacatgaac ctttaatccc ctgtcatgag ccatatacac cggcgccagc gccgtgccgt
     1381 agtcaacaca tgccagccac ccggcattgc agtgcgtcag gatattgacg gtttctccac
     1441 ctttcatgct gcttgcagct tcaattaccc gtagcccgtt caccccgatc agcctgcttc
     1501 tctcagcctc ctcatcacat agtccttccg ctgcacggca caatgcatca gcatccggga
     1561 agaatttcat cacccctgtc atgtaatcaa ctgcccatgc aaggttcacc gcggtcggtc
     1621 tggccgactt aagccgctgc gcgtcactga tcactgcatt ataaacattg ccggtttcgt
     1681 ttttcttccc gctgctgtgt tcaaggaatg aaagatatac tccccatgct gccgcagccc
     1741 caatgagagg agcccccctc accgccatct cccggatagc atcataaaca tcacttacac
     1801 tcctgagagt catcacctca aaggcaaacg ggagcctcct ctggtcaatc acatgtactg
     1861 acccgcggtc agccttatca tgccagatgc tcctgtaata cctttcccca accttcatca
     1921 actactcttc cctcacaaat taatcatcaa aattactaat aattacactt attctgatta
     1981 tataaacagt tacttaattc aatcacaacc attttcgaat atccgacccg tttgttccat
     2041 cacgttagaa ttaattgatt atctttgcat aagagtaaaa cctatggtag ataaagagga
     2101 atacagggca cggatcatcc ggacagctag caggattttc agtcattacg gcttcaggaa
     2161 gacgacgatg gatgaaattt cccgggcgct gaaaaaggga aagagttcaa tttattatta
     2221 tttcccgggc aaggaggaga tctttgaggc ggtggtgctt tatgaagcaa accagctgcg
     2281 caggaggctg acagctgcaa tcaaggaggt ggactcgccg ccggacaagc tgcgtaacta
     2341 tatatatgtg aggatgaggg agtttgcaaa gttgtcaaac tactacaatg ccgtctttga
     2401 caagaacctc gatcactacg agttcatcga aaagatcagg gcacgatatg accgggagga
     2461 gctggcaatc atccgcctgc tagtgtatgt aggaaacaac aggggtacat ttgccgtgga
     2521 ggacagtgag tataccgcca tggcgataca gacaatgctc aaggggatgg aggttccgct
     2581 attctggcgc aggcgcgaaa ctgacatcaa ttatcgcctc gaggcgatac tgaccctcat
     2641 ctttaacggt ataatgaaga aacagtaata acggagggct taccgcgatg cgggatcggt
     2701 cgctcatcat tttttcccgg ccacaggatt ttccggccgc aggctttttc cgccgcaggc
     2761 gatgatatca gctacttttt aggtacccct ttcttcggtg accactgttc ggtcaccggc
     2821 accggttccg gcttgcgggt gaatacccag acgagcagcg caatgcctgc cagcaccaac
     2881 gggatactca gccactggcc catgttcagc ttcatcccgg cctcgaaccc cacctggtct
     2941 tcctttatat attctacgag gaacctgaat ccgaagacaa aaatcaggaa cagaccgaac
     3001 agcagtccgg gcttctgttt cccgaggttc ttccagtaga tgcgcatcag tactccgaag
     3061 gtgatgaggt atgccagcgc ctcatatatc tgtgtggggt gctttggcgc cgtttctccg
     3121 ttccgaagga atacgaaccc ccatggcaga gttgtctcaa ctccgtaaat ttccgaattc
     3181 atcaggttcc ccaggcgtat cattgtcccg gccagtgcca ccacgattac caccctgtca
     3241 atgatccaca ggtacggctt tttctccttc cttacgaata gccacagtgc cagaagtatg
     3301 cctatggccg ccccgtggct tgccagtccg ccgtgccata ccttcagaat ctccaggggc
     3361 ctggccagat agtactcggg ttcatagaaa aagcagtgtc ccagcctggc gcctacaatc
     3421 gtgccaatgg ccatgtaaac ggtcagcctg tcgagggttg cgtctgtcag cccttcgttc
     3481 ctgaatatct tcctcatgat atagtacccg aagacgaacc ctgatgcaaa gagcagacca
     3541 taccatctta ctgcaaagtt gcctatccgg aagatctccg gattaacatt ccacggaatt
     3601 atctgaagtt ccatactgct gatgtttgga ttacaaattt atgcaaaaaa ggaacagttg
     3661 tcctatttca ctgtaaaagg catgtccgag ccccttcgcc acacatatca tgtaacaaaa
     3721 tttgatgtaa gtttgcaggt ggtggatcgc ggaagatata taacaggcag ccttggactg
     3781 acagtcctgg ccacgctttt tgtgctttcg tgtgcaaaga tcagcgctcc cacgggaggg
     3841 ccgaaggatc tggatcctcc tgtgatactg aaaagcattc cggagaacgg ctccgttctg
     3901 ttcaccggca agtcattcac cgtcatcttt gatgaatatg tcgtccttga ccgcataacg
     3961 gaaaaattca tggtgtcacc tccgctggct acaaaacccg acgtgaaact aaggggaaag
     4021 agccttgttg tcaactggga ggatgacctg gcagacagca ccacctacac tttttatttc
     4081 caggatgcca tccgtgacaa taacgaaaac aacccgatca acaattacca gtatgttttt
     4141 tcgaccggac cggttctcga ctcactctcg ctgaccggca acgtgttcaa tgcatctgat
     4201 cttgagatag ttgaagatgt gacagttatc atgtactcca acctgtcaga tactgcgccc
     4261 cgcaccatcc ttccggcata tatatccagg ccggatccgt cgggcgggtt catgataagc
     4321 aacatcaggc ccggaaacta caggctcttc gccctgaagg accttaacgg caataccagg
     4381 tacgatcttg ctgatgagat atttgccttc ggcgactctg tcatcgtcat cacccctgac
     4441 aactacttcg gccttgtgcc tgacactgtc acatacaggc ccgcaaatgc caccgagacc
     4501 accaaacccg ataagttcgt ttacgggctt cacaggctct atgcctttca ggagccgccg
     4561 aagcagcaat acctgacatt caccgagagg aagagctcat ggggtatcag cttcggaatg
     4621 gcattgccgt ctgacagcgg acaggtgtcg gtaaccctgc ccgaagctga cagttcatca
     4681 tggtacatgg agcacaacgt ggcacgtgat accttcttga tatgggtgac caatcccgat
     4741 gtgtacagcc gtgatctggt ggatgccatt ctcacctatc cgtttactga cagcaccggt
     4801 gcgattatcg cgaaaaccga caccatcagt ttcagatata tgaagcctgc cgccccgagg
     4861 ggaggcgccg gaaggacgcc gggactgagc atcaccacca atatttccgg aaagatcaga
     4921 ccgggcacag tgccgttgtt cagatcagcc acaccgcttg cagatcctga tacatcgctc
     4981 ataaggctga cccggacggt tgactcggtt caggttgtgc ttccttacga gtttgtaaga
     5041 gatacggtct cgtcccgcct cctgttcctg aaggcgcagc tcgagccggg agcgacctac
     5101 tcgctgctgt gcaggaaggg agctttcagc gatatcttcg gagtggccag tgactctgcg
     5161 atgtaccgaa tctctgtagc tactgaagag gattacggaa gcctcaggat taagattgcc
     5221 ggctatgagg gaagggtgat aattcagctg accgcggatg ctaaggacaa ggtgatacgt
     5281 gaagagtcac ttaccagccc cggagaggtc tcgttcccgc ttcttgaaaa agggaagtac
     5341 aggctgaagg cgatctacga tcttgattcg gacgggaaat ggaccaccgg ggactttaat
     5401 gccggaaggc tgcccgaacc ggtcacatat tacccggctg agctggaagt caagattaac
     5461 tgggccctgg tgcaggactg ggatatcgga gagatgtatg tcaaggatgt ttcgctgcgg
     5521 aataagcccg caacaaggag gtaaacagat gtcgacacgc aggaagttta tcaggaacag
     5581 catggtggca ggggcggcgt tctctgccgg gataccttca tttgtctcag ccagtaaccg
     5641 gaagaggcct tcggtaagac ccgctgccag ggtgcgaaaa ttccgcagca aggctgtaga
     5701 ggagactatc aaagagattg agtcattcct ggggtctgac aatgagctgg catggctctt
     5761 ctcgaactgt tttcccaaca cccttgacac cactgtcaac ttttccgaaa aggaaggcag
     5821 gccagatacc tttgtcatca ccggtgatat caatgcgatg tggctgcgtg actcctcggc
     5881 ccaggtgtgg ccctatgtgt cacttgcccc gggtgacccg cagctgaagg atctcattgc
     5941 cggtgtgatc aacaggcagg ttaagtgcat tcatattgat ccttatgcca atgccttcaa
     6001 cgatggtcct ggccagagcg aatggatgaa tgacctgacc gacatgaaac cggagctgca
     6061 tgagcgcaag tgggagattg actcactctg ttattctgtc aggctggctc acgggtactg
     6121 gaaagccacc ggagacacct ctgtttttga ttcctcgtgg tcatcggcga tgaggctgat
     6181 catcaaaaca ttccgcgaac agcagcgcaa ggacaatccg ggtccctaca agttccagag
     6241 ggttacaggc tggcagaccg atactgttgc cggagccggt tatggcaatc cggtcaatcc
     6301 ggtgggcctg atcgtctcaa tcttcaggcc gtcagatgat gcaaccatat ggcctttcct
     6361 ggttccttca aactatttcg cggtgagcgc actgcgccag attggcgaga ttgccgccga
     6421 tatcacccgt gatgagactc tcctggttga tgccaccgcg ctggccgatg aggtagatgc
     6481 cgcacttgcg aaatatgccc gtgccgctca cccggagcac ggagagatca tcccctacga
     6541 gactgacggg tatttcaact acaatttcat ggatgatgcc aatgttccca gcctgctcag
     6601 catgccatac cttggaacca ttgaccggag tgatccgctt tacctgcgca cccgcaagtt
     6661 tgtgctgagc aaaagcaacc cgtactattt ttcaggcatc agcgggcatg gtgtggggag
     6721 tccgcatacc ggccttgacc gcatctggcc cattgccatc atcatgaagg cgctgacagc
     6781 ctcgaacaat tatgatatca tcacatcgct caggatgctt aaaaataccc atgccgcaac
     6841 gggcttcatg catgagtcgt tcatgaagga cgattcctcg aagttcacac gcaaatggtt
     6901 tgcatgggcc aacaccctgt tcggggaact ggttttgcat acttacagga cacagcctga
     6961 gctgcttaag gaagtataaa atccataaca tatgaaagaa gtagttgccg gagttgacat
     7021 tggcggaaca aacaccatga ttggttttgt tgatgctgat ggtgtcattc atgccgaggg
     7081 gaggctctcg acgcccgatt atgcctttgc agatgatttt gtcattgcgc ttgacgcgaa
     7141 gctgaaggag atggtggctg agaacagcgg actgaagctg atcggcatag gtatcggcgc
     7201 accgaatgcc aacttcaaca aggggacgat tgagctggcg cccaacctgc gttggaaggg
     7261 gatcatcccg cttgtcagga taaccgagaa gctcaccggc ctcaagaccc gcatcaccaa
     7321 tgacgccaat gcggcagctc ttggagagat gatctttggc gccgcgaagg gtgtgaagga
     7381 cttcatcatc ctgaccctgg gcacgggcct cggcagcggc atcgttgttg acggcgaggt
     7441 ggtgtacgga cataccggtt ttgccggtga gctgggtcat atgaccgtgg ttgagggagg
     7501 ccgggtgtgc ggatgcgggc gccgcggctg tcttgagacg tatgcatcag ccacagggct
     7561 ggtccgtaca gttttatcac tgctcagcga ccgccgtgat gagagcctgc tgcgggaact
     7621 caggggcagt gagatcacct cacggcggat cgctgaagcc gcggcggccg gcgatatcat
     7681 cgctcaggaa gctcttgacc agaccgcccg gatgatcgca ctcgggatag ccaactcaat
     7741 tatcttctcg agcccggagg tcatttacct gttcggcgga ctggcaaagg caggcgacgc
     7801 cctcttcgtg ccggtgcgga agtatgctga cgagctggtg cagcccgtct tccgcggcac
     7861 cttcagagtg gaaccctcag ggttacccga ggcgcaggcg gcaatactcg gtgcatcggc
     7921 cctgatatgg aaagaagcga gatagaagca gtcggcagca gatactgcga cttgcgattt
     7981 gcgacgttcg attttaaccg cagaccgcgc aacattgcat atttttggtt atgaattaca
     8041 ttcagatacc gcataactgt cacagcatcg aaacctcaca ccaccttctt cccgaacaaa
     8101 cctatgtcac acataacaat agctagaaat gcgtatcatt gaggtcagaa ggctgaagac
     8161 agacagggaa ataccaccaa tggcagaggt gtctgcccgg ctcgacgaac tgggcgaagg
     8221 ccacaggata ggaacagtga actggcagag ccatacatac aaacctgaag tgagattcaa
     8281 tatcgcctgg ggcgaaaagg aaatctacat caagtactat gtccgcgagg cacatgtcaa
     8341 agccgaaaag ggcaccacca acgagatggt atgcgaggac tcatgtgtag aattcttcgt
     8401 ctcaccggaa gatgacggca tctattacaa cttcgagttc aatcccatcg gtaccacgct
     8461 gatgggctcg ggtcacggcc gccatgacag cgcacgcaca gatccggcca ttgtcaacaa
     8521 catccgccga ctcaccacca tgggcagcga accgttcccg gagattagcg gagacatcag
     8581 ctggtcaatc accgtggcca tcccccttgg aactttcttc aggcatagtg tcggcagcct
     8641 ggcaggcaaa agtttcaggg caaacttcta caagtgcggc gacaagctga ccaccccgca
     8701 cttcgtcaca tggaacccgg ttggcacgga aaaacctgat taccacaggc cggaattctt
     8761 cggaattctg aagtttatat aaccatttca gtgtcttctt ctgcaagtga actgaattca
     8821 aacaaaccac accataaaac ctgattatga tcaagggaaa tgcagtcata ggccagtcgg
     8881 gcggaccgac ttcagtaatc aactccagcc tcgccggtgt gatagacgcc gcaaaaagat
     8941 caggcctggt atcggcggtt tacggcatga attacggaat agaagggttc atgaacgagt
     9001 ggctctttga cctctcggcc cagccggccg agatcattca tggtttgcgc cacaccccat
     9061 cctcggccct gggatcatcc cgctacaagc tgaaggatga tgatttcccg aagatactga
     9121 gcgtgctgca gaaatttgac atcaggtatt tcttcctgat cggagggaat gacacaatgg
     9181 acacagtcaa cagggtggag gcatattgcc gcaccatggg ttatgagctt accggtgtcg
     9241 gcatacccaa gacagttgac aatgacctgt tcggcactga ccataccccc ggttttgcct
     9301 cggcggcatg gtcaaacata ctgaacgtga tgcaggcagg caccctggcc cgtgacatga
     9361 tgaaggttga ccagtttgtt gtctaccaga ccatcggccg cgatgcggga tggctggctg
     9421 ccgccacggc cctggcacgc cgcgatgagg atgaagcccc gcacctcata tatacacccg
     9481 aacatccttt tatcagggaa aagttccttg ccgatgcaga tcactgcatc aggaggtacg
     9541 gctgggtctt cgtcgttgcc ggcgagggaa tcaaatatga agacggcacg ccggtcagcg
     9601 cttcggtggt gaaggacaaa ttcagcaaca cggagtttgg agccatgggg ggcgccagcg
     9661 tggccatcac ccttcaccgt atgctttcat cagagttcgg catcaggggc gagttccagg
     9721 tgcctgagtc gcttataatg agcgattatg tcagggcgct tcccgccgac cttgaggagg
     9781 cttaccgctg cggcatcgag gctgtccgca tggccgaaag gggtgagtcg gggaagatgg
     9841 tgacaatcga aagaaaatca gatgacccgt atgaggtcgt ctacggaatg gttcccctcg
     9901 gcaaggtggc tatcgccgcc aggcccatgc ctgaagagta tttcaatgct gcgggcaatt
     9961 atgtctctga cgcttttgcc aggtatatga agcctttggc cggagcaatt ccggagtttg
    10021 tgagactgaa aaagatcatg gtaattaaat aaaacctaaa gcaatggcaa ctgagttcaa
    10081 caaggtggcg ctctcaatca acgcacaccc cgatgatgcc gaggcatgga atgccggggc
    10141 attgaagctg ctgcatgaca gggggtataa gattgtgatt gcaaccatga ccgggggtga
    10201 ccttggaggg tgcaacatga gcatggagga gacggcacgg gtacgctatg aggaggcaaa
    10261 ggccgcagcc gcagtgcttg atgcagagta ctataccatg ggaggcattg acggttcgct
    10321 gtttgacagt cgcgagatgc gcctgaaggt gatctcgctg atgcgcaggg tacgtgccgg
    10381 tatcgtcttc acccacctgc ccaatgacta tcatcctgac cacagggcaa cggctgccat
    10441 agcagaagcc gctgcaatgg tggcctcgct tgacccggtg cctgttcctg agaaaccatt
    10501 ggaggtcacc cccctccttt atcatacctc gcccctgacg ctgagtgatc cgttgggatc
    10561 gcagcttacg ccgccacatt tcttcgttga tgtcaccagt gcggtgcaga ccaagatcga
    10621 gatgctgggc aggcatgtct cacagcttga gctgatgaaa cacatgcaca agatcgatga
    10681 tttcttcggc tatgtactgg agggtaacaa aaactacggt aaattagcgg
//
LOCUS       DAOPXU010000095        10721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_7100_length_10721_cov_12.263923, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000095 DAOPXU010000000
VERSION     DAOPXU010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7100_length_10721_cov_12.263923"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1613)
                     /locus_tag="PKK03_09425"
     CDS             complement(90..1613)
                     /locus_tag="PKK03_09425"
                     /inference="COORDINATES: protein motif:HMM:NF010184.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HNX84661.1"
                     /translation="MTDLYYTGFDNEKYLIEQTASILERVERFNNKLYLEFGGKLLYD
                     YHASRVLPGFDPNVKMRLLQMLKEKIDVILCIHAGDIERKKVRADFGITYDVDALKTI
                     DDFREWGIDVTAVVITRYTGQQPARAFRNKLELRGVKVYTHTPTKGYPTDVDLIVSDE
                     GYGANEYIKTTHPIVVVTGPGPGSGKLATCLCNLYHEYRHGVKAGYAKFETFPIWDLP
                     LDHKVNIAYEAATLDLKDEVLVDSYHLKRYGQNVVNYNRDIEAFHLLKRILEKITGGE
                     SMYQSPTDMGVNRASAGIINDVLIQEAAHQEIIRRYFRCAVEYAIGLADKEILEHAGK
                     IMGKVKAKPEDRRVVMPARRAAAEAEKGGKGNGGIFSGAAIELGNGTIITGKNSPLMH
                     SASSLILNATKHMAGLPDSLLLLPKTLIDSVTYLKKDILNGKEVSLDVEETLILLGIS
                     ALFNPAAQAALEQLTVLRGCEVHLTHIPTPGDEAGLRKLNVNVTCDPEYSSKSLFVS"
     gene            complement(1780..2625)
                     /gene="ygiD"
                     /locus_tag="PKK03_09430"
     CDS             complement(1780..2625)
                     /gene="ygiD"
                     /locus_tag="PKK03_09430"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HNX84662.1"
                     /translation="MARLLSRMHDEGLSFAGDGYHSEKMPVLFVGHGSPMNAIEENEF
                     AAGWREMGSKLPRPAAILCVSAHWQSKGTKVTAMEKPETIHDFGGFPRELYEVQYPAP
                     GSPELARTTATTVHDRVIELDKTWGLDHGAWSVIRRMYPAADIPVVELSLNYHLTPQQ
                     HYDLAREIAPLRRKGVLIIGSGNMVHNLGLVAWDHVNTPGYAYDWAIEADAAIKKSIL
                     GGDHAPLINYQAQGRAFRLAIPTPEHFIPLLYSLALQEKDEPVELFNDKPVGGSLTMT
                     SVKIG"
     gene            complement(2683..3792)
                     /gene="menC"
                     /locus_tag="PKK03_09435"
     CDS             complement(2683..3792)
                     /gene="menC"
                     /locus_tag="PKK03_09435"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HNX84663.1"
                     /translation="MRITQVELRHIKMVLVSPFVTSMGTEYDEEHIIVRVDGEGITGW
                     GESVAEGTPYYSYETVQTAWHILRDFLIPSILGKDLKGVDEAIASYAIVRGHMMAKAG
                     LEAALWDAFARSENRSLSRMLGGVRDRVDVGVSIGIQPTEKDLIKKVEGYLAEGYKRI
                     KIKIAPGNDIQYVSALRNEFPRLLLQVDANSAYTLNEIDLFKRLDEYNLSLIEQPLGY
                     EDIYDHSKLQREIKTPICLDESIHSLDDTRAAIELKSCRIINIKPGRVGGFTESKLIH
                     DYCASMMIPVWHGGMLESGIGRAGNVALASLPNFILPGDISASKRYYKEDIVIPEFVV
                     NSDGTINVPSGPGIGVEVDMKMLEKVTVRKEVFRA"
     gene            complement(3838..5334)
                     /locus_tag="PKK03_09440"
     CDS             complement(3838..5334)
                     /locus_tag="PKK03_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNX84664.1"
                     /translation="MNRLIIFCFLVGCYSVSAQDFQTPLQKNGFTRPSSYEELSEFVR
                     QADAGSGLLTVEAAGNSVRGRNIYAMKFSSSVFGVDRSKIRVFLFAQQHGNEHSGKEG
                     ALLLAAELLKPENRYLFDRIDLLLVPQVNPDGSEESQRRNGNEADLNRNHLILTEPET
                     ITLHRLFDQYHFDVTMDVHEYYPYGETWKEYGYRNNSDELLGTATNINVSEKLRRLSN
                     ESFLPFMKEFFSGRNVSNFIYSPGGPPEIDYIRHSTFDINDGRQSFAILNTLSFIQEG
                     INGRDLFTDNIRRRAEGQMTGMRGLLEYSYDNYRKIKKTVAAERKKLIRGVAGKTVTI
                     QNEHVRDGRTLPLPLWSYSTGTDTVVNVIDYRPVVRSLYDVRKPCGYMIPAELVDVTA
                     WIARHALVTENFAGGDRYRIEQYDVSSIDSTDFEGDMIVNPEVTLREHDAGIKYSDYI
                     YVPTSQLKGNMIVIALEPKSMLGLVTYPGFANLLRANDKFPILRVTRK"
     gene            complement(5362..6525)
                     /gene="iadA"
                     /locus_tag="PKK03_09445"
     CDS             complement(5362..6525)
                     /gene="iadA"
                     /locus_tag="PKK03_09445"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HNX84665.1"
                     /translation="MLLIKNAEVHSPVFLGIKDILIAGEKIIAVENEIDARALKSIEI
                     ITIDGTGKRVIPGLIDAHVHVAGAGGEGGPATRTPEMQLSHMLDGGITTVVGCLGTDG
                     FTRSLEAVLMKVKSLKAQGVSAYMYTGSYQVPTPTLLGDVGRDIALIEEVIGIGEIAI
                     SDHRSSCPSTAELIRLVEHARVGGMLGGKAGIANMHMGDARDPFRPIYDAVENSELKL
                     TQFFPTHCNRNPYIFEDAKAYGKKGYVDLTASSYPYDPENEIKPSKAIVELLSAGVPL
                     EHITLTSDGNGSLPNFDREGRLISMDMGLPKSIMSVMIDTVTEENLPLETAVAVVTSN
                     VASILRLRDKGRIEPGKDADIVILDKDYAISDLISRGQMMTRNYERLKKGTYE"
     gene            complement(6559..8163)
                     /locus_tag="PKK03_09450"
     CDS             complement(6559..8163)
                     /locus_tag="PKK03_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HNX84666.1"
                     /translation="MAKKRQIPHTFVLVFSIIVLAAAASWFVDGGEYSRTLKVMPDGT
                     TRTVIDPGSYQNVENNPQTWQVFSALFEGFVAKSDIIIFILLIGGAFWIMNESKAIDV
                     SIIAFLGVARRLERFRFIKWLGIDNIIITLFMILFSLFGSVFGMSEETIAFIIIFVPL
                     AISMGYDSIVGICMCFFAAGLGFAGATLNPFTIGIAQGLSDLPLFSGLEYRIFMWVVL
                     TLTGILYVLWYARRIKRDPKRSVMYELDDVWRKMVLQRETKTAYTTPVSAWVVYIVLF
                     AVMVVFAVLYPVSTISFGQAKLTIPALPVIAGLFLISAYVALRKSVHFFILDIFIFTI
                     LFLLVGVMGYHWYVMEIATLFFVMGLSSGAAMGYSPNKITELFIAGVKDILSAALVVG
                     LAGGIIVVLQNGKVIDAMLHGIAESMSDVGKFGSLSIMYGIQTLINLVMTSGSAKAAL
                     TMPIMAPFSDLIEVSRQATVVAYQLGDGITNMITPTSAVLVGVLGVAKIPYEKWFRWA
                     WPLILFLMILGLLLLLPTATLDLNGF"
     gene            complement(8175..9023)
                     /locus_tag="PKK03_09455"
     CDS             complement(8175..9023)
                     /locus_tag="PKK03_09455"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNX84667.1"
                     /translation="MLKSIAVRMMLLTALCAAGYSCRSETPKEGKLFILGGGNLSSSI
                     ISKIVAESGLDKGGYGVILPMSSEEPDTAIFYTTAYFTKQGLNNVHGLHFVRGKPYSQ
                     ARIDSIRNARMVFISGGDQSRFMEAIEGTPIKAAIHEAFEKGNVVCGTSAGAALMSKT
                     MITGNELKNPDADVVFATMEAENVETIEGLGMLEKVIIDQHFIIRKRLNRSVCACIEH
                     PEQMVVGIDEPAGIFVEGNTATVLDAQVVVLRNPVDPKSENGLLGARNIQLDVYLPGE
                     SFRILK"
     gene            complement(9036..>9386)
                     /locus_tag="PKK03_09460"
     CDS             complement(9036..>9386)
                     /locus_tag="PKK03_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pkd domain containing protein"
                     /protein_id="HNX84668.1"
                     /translation="LWSKNLDGTGETEIIPLVEGGSTIVIGSRIYFDQWVASGDIHIK
                     SANPDGTGISTVATGVSRVVFGLGYEAEYDKIYWGDRTPGKIMRANRDGSGIETWYVK
                     SGSSPRGIEFGKKD"
     assembly_gap    9387..9486
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9487..9859)
                     /locus_tag="PKK03_09465"
     CDS             complement(<9487..9859)
                     /locus_tag="PKK03_09465"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX84669.1"
                     /translation="MKFVSRIVIVLSLSLLLNSCKDEGFPVPPASTVPRFSYTVDNNG
                     YAPATVNFTNESIVPAEAGNPFYYWDFGDGTRSTDVSPEHLYSASGVFTASLMIKTDV
                     SMEIKQQSEEIVILNSEVTGIP"
     gene            complement(9990..>10721)
                     /locus_tag="PKK03_09470"
     CDS             complement(9990..>10721)
                     /locus_tag="PKK03_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84670.1"
                     /translation="KIVLDGTNTLSTVKDVAGMSGPGIAYDDETGYLFFSDYYDADTP
                     DGKIWKMKPDGTGLASIVTGILDPYGVAIDHDNNKVYWADDAEHISRANLDGSSLQTE
                     LVTIEEASMRAVAIDEKNDKLYFYEAWFENLYVANLDGTNPTILVSGVYGYSLFVDTE
                     NNKIYFDDQNSMTLKRANLDGSGVVTIADVDDRVFGIAIDYEKDKLYWSDRGLGEIYE
                     ANLNGTGKVTLATGLSSPRGIVLVK"
BASE COUNT         2541 a         2926 c         2447 g         2707 t
ORIGIN      
        1 gaggaaccgc cgccggcggg accgaaatcc cgatgcccga agggtgtcgg gaccctcaga
       61 ccctcagacc ctcagacctt cagaccttct caagacacaa acaggctctt gctggaatac
      121 tccggatcac acgtcacgtt aacattcagc ttgcgcaggc cggcctcatc acccggggtg
      181 gggatgtgcg tcaggtgcac ctcgcagccg cgaagcaccg tcagctgctc aagcgctgcc
      241 tgtgcagcgg gattgaacag cgcactgatc cccagcagaa tcagcgtctc ctccacatcc
      301 aggctcacct ccttgccgtt gagaatatcc ttcttcaggt aggtgaccga gtcaatgagg
      361 gtcttaggca gcagaagcag gctgtcaggc aaccctgcca tatgcttcgt ggcattgagt
      421 atcaggcttg aggctgaatg catcagggga gagttcttgc cggtgatgat cgttccgttg
      481 cccagttcaa tcgctgctcc gctgaagata ccaccgttgc ccttgccgcc cttctccgct
      541 tcggcggcag ccctgcgtgc cggcatcaca accctgcggt cctccggctt cgccttaacc
      601 tttcccatga tcttcccggc atgctccagt atctccttgt cagccagccc gatggcatac
      661 tccacggcac acctgaagta ccttcgtatt atctcctggt gcgcagcctc ctggatcagg
      721 acgtcattta taattcccgc actggcacga ttgacaccca tatcggtggg cgactggtac
      781 attgattcac ctccggtaat cttttcaagg atcctcttga gcaggtgaaa cgcctcgatg
      841 tcgcggttgt agtttacaac gttctgtccg taccgtttga ggtgatagct gtcaacaagc
      901 acctcgtcct taaggtcgag ggtggcggcc tcgtacgcga tgttcacctt gtggtcaagg
      961 ggcagatccc agatagggaa ggtctcgaac ttggcataac cggccttaac accatgccgg
     1021 tactcgtggt agaggttgca cagacaggtg gccagcttgc cgcttcccgg ccccggcccc
     1081 gtcaccacta caatcgggtg ggtggtttta atatactcgt tggcgccgta cccctcatca
     1141 ctgacaatca gatccacatc ggtgggatat cccttcgtcg gtgtatgggt atacaccttt
     1201 actcccctga gttccagctt gttcctgaaa gccctggccg gctgctgtcc cgtgtaacgg
     1261 gtgatgacga cggctgttac atcaatgccc cactccctga aatcatcaat ggtcttgagc
     1321 gcatccacat cgtaggtaat gccgaaatca gcccgcacct tctttctctc gatatcgccg
     1381 gcatgaatgc agaggatcac atcgatcttt tccttcagca tctgcagcag cctcatcttg
     1441 acgttcgggt cgaaacccgg aaggactctc gatgcatgat agtcataaag cagcttaccc
     1501 ccgaactcga ggtaaagctt gttgttgaaa cgctctaccc tctcaaggat ggatgcggtc
     1561 tgctcaatga ggtatttctc attgtcaaac ccggtatagt ataaatcagt catttagctc
     1621 tccccctgaa taattccgac aacaatcagg atcagcttcc aatattaagt aaattttctt
     1681 tgtatgttgg attcagccgc gacaaaaaat attcaccgca gggggttgaa aacttaattg
     1741 ccatgcggcg aagctcctgt cgccggggcg aagctcctgt cacccgatct tcaccgaggt
     1801 catcgtcagc gatcccccga caggcttgtc attgaacagt tccaccggtt catccttctc
     1861 ctgcaatgcc agcgaataga gcaaagggat gaagtgctcc ggggtgggaa tggcgagcct
     1921 gaaagccctg ccctgggcct ggtaattgat aagaggggca tggtcgcccc cgaggatgct
     1981 cttctttatt gccgcatcgg cttcaatggc ccagtcatat gcatatcccg gcgtgttaac
     2041 gtggtcccac gccaccagcc ccaggttatg caccatattg ccgctgccga tgatcagcac
     2101 ccctttcctg cgcagcggcg caatctcacg tgccaggtca taatgctgtt gcggagtcag
     2161 gtgatagttg aggctcagct ccacaaccgg gatatcggca gcggggtaca tccgcctgat
     2221 cacactccag gcaccatggt caagacccca ggttttgtcc agctcgataa ccctgtcatg
     2281 caccgtcgtg gcagtggtgc gtgccagctc cggactgccg ggcgcaggat actgcacctc
     2341 gtaaagttcg cggggaaacc ccccgaagtc gtggatggtc tccggctttt ccattgctgt
     2401 tacctttgtt cccttcgact gccagtgagc cgaaacgcac agtatggcgg ccggacgcgg
     2461 aagcttgctg cccatctcgc gccatcctgc ggcaaattca ttctcctcga tggcattcat
     2521 cgggctgccg tgcccgacga acagcacggg catcttctct gagtgataac cgtcaccggc
     2581 gaaactcagc ccctcatcat gcattcttga aagcaatcgt gccatagccg tcttttgtta
     2641 ttcaacaatg caggccggga aaagttaaac aggactatta tctcatgctc tgaatacctc
     2701 ttttctgact gtcacttttt cgagcatctt catgtctacc tcaacaccaa tgcccggccc
     2761 tgacggcaca tttatggttc cgtcactgtt cacaacaaac tcaggaatga ctatgtcttc
     2821 cttataatac cgtttgctgg cagagatatc gccgggaaga ataaaattcg gaagagaagc
     2881 cagggcaaca tttccggccc ggccgattcc cgactccagc atccctccgt gccatacggg
     2941 aatcatcatc gaggcacagt aatcatgaat cagtttcgac tcggtaaaac cgccaacccg
     3001 cccgggcttg atgtttatta tcctgcagct tttcagttca atcgcagccc tcgtatcatc
     3061 gagtgaatga atgctctcat caaggcagat gggcgtcttt atttcacgct gtagctttga
     3121 atgatcataa atatcctcgt atccgagagg ctgctcgatc agcgaaaggt tgtactcatc
     3181 gagtcttttg aacaggtcaa tttcattgag agtatatgct gagtttgcat ccacctggag
     3241 gagcagccgg gggaactcat tacgcagagc acttacatac tgaatgtcat ttcccggggc
     3301 aatcttgatt ttgatcctct tatatccctc tgcaagataa ccctccacct tttttatcag
     3361 atccttctcc gtcggttgta tcccgatact cacgccaaca tcaaccctgt ctctcacccc
     3421 gccaagcatc cgcgaaagag acctgttctc cgacctggca aatgcatccc acagggcagc
     3481 ctccagtccc gcttttgcca tcatatgtcc tctcacaatt gcataggagg caatagcctc
     3541 atcaacccct ttcagatcct ttcccaggat ggaggggatc aaaaaatcac gaagaatatg
     3601 ccaggcggtc tgcactgttt cataagagta gtaaggagtc ccctcagcaa cgctttcacc
     3661 ccagccggtg atgccttcgc cgtcaacgcg gacaattatg tgttcctcat catactctgt
     3721 ccccatggat gtgacaaacg gactgaccag gaccatcttg atgtgcctga gttctacctg
     3781 tgtgatacgc atttcgagtt tttttagagt taagtaacaa tgatggtacc gggcgggtta
     3841 ctttcgcgta acccggagta tcgggaactt gtcgtttgcc ctgagcagat tagcaaaacc
     3901 cggatatgtc acaagaccca gcatcgattt gggctcgagg gcaatgacta tcatattccc
     3961 ctttaactgt gatgtgggaa catatatata atcgctgtac ttaatcccag catcgtgctc
     4021 acgcaaagtc acctccgggt tcactatcat atctccctca aagtctgttg aatcaattga
     4081 ggaaacatcg tactgttcta tcctgtaacg gtcaccgccg gcaaagtttt ctgtcacaag
     4141 agcatgtctg gctatccagg cggtaacatc aaccagctct gcggggatca tgtaaccgca
     4201 tggtttcctg acatcataca gtgatctgac cacaggcctg taatcaatta cattcaccac
     4261 ggtatcggtg cctgtggaat atgaccagag tggcagcgga agcgtacggc cgtctctcac
     4321 atgttcattc tgtatcgtga cggtttttcc agccacgcca cgtatcagtt ttttcctctc
     4381 agcagctacc gtttttttga ttttccggta attgtcatac gaatactcca gcagtcccct
     4441 catccccgtc atctgtcctt cggcccggcg cctgatgtta tcggtaaaaa gatccctgcc
     4501 gttaatccct tcctggatga atgaaagagt attaaggatg gcaaagctct gcctgccgtc
     4561 attgatatca aatgtgctgt gcctgatata atcgatttcc gggggccccc cgggactgta
     4621 aatgaaattg gaaacatttc tcccggaaaa gaattctttc ataaatggaa gaaaactttc
     4681 attggatagt cgcctcagtt tttctgagac gttgatattg gtggcagttc caagcagttc
     4741 gtccgagttg ttgcggtaac cgtattcttt ccatgtctca ccgtacgggt agtattcatg
     4801 aacatccatg gtcacatcaa aatgatactg atcgaagagc cggtgcaggg tgatggtttc
     4861 cggttcagta agaatcaggt gattgcggtt caggtcagct tcattgccgt tacggcgctg
     4921 actttcttct gatccgtccg gattcacctg gggcaccagc aacaggtcaa tacggtcaaa
     4981 caggtaccgg ttttcaggtt ttaaaagttc tgcggcaagg agcagggctc cctctttccc
     5041 tgaatgttcg tttccatgct gctgcgcgaa aaggaacacc ctgatcttcg acctgtctac
     5101 tccgaataca gaggaggaaa acttcatcgc ataaatgttc ctcccccgga ccgagttccc
     5161 ggctgcctca accgtgagaa ggccggaacc ggcgtcagcc tgcctcacga attcactcag
     5221 ctcttcatag gacgaaggcc tggtaaaccc gtttttctgc aaaggtgtct gaaaatcctg
     5281 ggctgataca gagtagcagc caaccaggaa acagaagatg ataagcctgt tcattttatt
     5341 aatatcgaag catatggaat actattcata ggtccctttt ttcaatcgct cgtaattgcg
     5401 ggtcatcatc tgtccgcggg aaataagatc agagattgca taatccttgt cgagaatcac
     5461 gatgtcagca tctttcccgg gttcaatcct tcccttgtcc ctgagcctaa ggattgaagc
     5521 aacgttggag gtcaccaccg caactgctgt ttcaagggga agattttcct ctgtcacggt
     5581 atcaatcatc accgacatga tcgatttcgg taatcccatg tccatgctga tcagcctgcc
     5641 ctccctgtcg aagttaggca gtgatccgtt tccgtctgat gtcagtgtga tatgttccag
     5701 cggcactccg gccgataaaa gctcaacgat tgccttcgat ggcttgatct cattttcagg
     5761 gtcatatggg tatgagctgg ctgtcaggtc aacatatccc ttcttcccgt aggcttttgc
     5821 gtcttcgaaa atatagggat tcctgttgca gtgtgtcggg aaaaactggg tcagcttcaa
     5881 ttcgctgttt tcaacagcgt cataaatcgg ccggaaggga tcccttgcat cacccatgtg
     5941 catattggca atacctgctt ttcctccgag catgccgccc acccttgcat gttccacgag
     6001 acggataagc tccgcggtgg aaggacagga gcttctgtga tcagatatgg ctatttctcc
     6061 gattcctata acctcttcaa taagggctat atcccttccc acatcgccaa gcaatgtggg
     6121 tgtgggaacc tggtatgaac cggtatacat ataagctgat acaccctggg ccttcagcga
     6181 cttgaccttc atcaggaccg cttcaagtga tcttgtgaac ccgtcggttc ccaggcatcc
     6241 tacaacagtt gttataccgc catcgagcat atgtgacagc tgcatttccg gagtacgggt
     6301 ggccggccca ccttcaccgc cggcgccggc aacatgaaca tgggcgtcga taagccccgg
     6361 gatcactctc tttccggtac cgtcaatggt tatgatctcg atactcttca atgcccttgc
     6421 gtcgatctca ttttcaacgg caatgatttt ttctcctgct ataaggatat cttttatgcc
     6481 taaaaaaacc gggctgtgca cttcggcatt ttttatcagc agcatgttca taatcagttt
     6541 tttagtaatg aaaaacagct agaatccatt tagatccagt gttgcggtcg ggagcagcag
     6601 caacagtccg aggatcatta agaacagtat caggggccat gcccacctga accatttttc
     6661 gtaagggatt ttggcaacac ccagcacacc gaccagcacg gcagaggtgg gggtgatcat
     6721 gttcgttatg ccgtcgccaa gctggtaagc cactacagtc gcctgccgcg aaacctcaat
     6781 aaggtctgag aacggcgcca tgattggcat ggtcagtgct gccttggctg acccggaggt
     6841 cattacaaga ttgatcaggg tctggatccc gtacatgata ctaagcgagc caaatttacc
     6901 gacatcgctc attgactctg caatgccgtg cagcatcgca tcaatgacct tgccgttctg
     6961 caatacaaca attattcctc cggccagacc aactacaagg gcagcagaca gaatatcctt
     7021 tactccggca atgaacagct ccgtaatttt attcggagaa tagcccattg ccgctccgct
     7081 cgaaagcccc attacaaaaa acagggttgc aatctccatc acgtaccagt gatagcccat
     7141 aacccccacc aggagaaaca ggatggtgaa gatgaagatg tcaaggataa agaaatgcac
     7201 tgacttgcgg agagcaacat aagctgatat caggaacagc cccgcaataa ccgggagagc
     7261 cggtattgta agttttgcct gcccgaaact gatagtcgaa acaggataca aaactgcaaa
     7321 gacaaccatt acagcaaaga ggacgatgta cacgacccag gcagagaccg gggttgtgta
     7381 tgctgttttt gtctccctct gcagaaccat cttccgccat acatcatcaa gctcatacat
     7441 aaccgatctt ttcggatcac gtttaatcct cctggcgtac catagtacat acaatatgcc
     7501 cgtcagggtg agcacaaccc acatgaatat gcggtactcc aggcctgaaa acagcggaag
     7561 gtcagataat ccctgggcga tgcctatggt gaacggattc agggttgcgc ctgcaaaacc
     7621 cagcccggca gcaaaaaaac acatgcatat ccccactatc gaatcatacc ccatgctgat
     7681 cgcaagaggc acgaagatga taatgaatgc gatggtctct tccgacatcc cgaacactga
     7741 tccgaaaagg ctgaagagta tcataaagag ggtaatgatg atattgtcta tgcccagcca
     7801 ttttatgaac ctgaatctct ccagccgccg ggcaacaccc agaaaggcaa taattgaaac
     7861 atcaattgcc ttgctctcat tcataatcca gaacgcacca ccgataagca gtatgaatat
     7921 tattatatcc gacttggcta cgaatccctc gaacagggca gaaaaaacct gccatgtctg
     7981 aggattattt tcaacgttct gatatgatcc ggggtcaatg acagtcctgg tagtaccgtc
     8041 aggcattact ttaagcgtcc tggaatattc acctccgtct acgaaccagc tggcagcggc
     8101 agccaataca ataatagaaa agacaaggac aaaagtgtgg gggatctgtc tctttttcgc
     8161 catttatcag ttcctcattt taatatccgg aagctttctc ccggcaggta gacatcaagt
     8221 tgaatattcc gggctcccag caatccgttt tccgatttcg ggtcaaccgg attccgaagc
     8281 accaccacct gggcatccag aaccgtggct gtattgcctt ccacgaaaat acctgcaggc
     8341 tcgtctattc ccaccaccat ctgttcaggg tgctcaatgc atgcgcagac cgacctgttc
     8401 aggcgcttcc ggataatgaa atgctggtca atgatcacct tttcaagcat cccgagaccc
     8461 tctatggttt caacgttctc cgcctccata gtggcgaaca caacatcagc atccggattt
     8521 ttcagttcat tcccggtgat catggtcttg ctcatcagtg ctgctcccgc gctggtaccg
     8581 cataccacat ttcctttttc aaaggcctca tggatggcag cctttatggg agtaccctca
     8641 atcgcctcca tgaagcggct ctgatcgcct cctgaaataa aaaccattct tgcattgcgg
     8701 atggaatcga tcctggcctg tgaatatggt ttacccctga caaaatgtag gccatgcaca
     8761 ttgttcagtc cctgtttcgt gaaatacgca gtagtgtaaa aaattgcagt atcaggctct
     8821 tcacttgaca ttggaaggat aacaccgtac ccccctttgt ccagtcctga ttctgcaaca
     8881 attttcgaaa tgatggagga ggagaggttt cctcctccga gaatgaacag ttttccttct
     8941 ttcggggttt cgctccggca cgaataacct gctgcgcaca atgccgtcag cagcatcatg
     9001 cgtaccgcta ttgattttaa catacagtct cttttttaat ccttcttgcc aaattctatt
     9061 ccgcgcgggc ttgatcctga tttaacatac caggtctcaa tacctgatcc gtcacggttc
     9121 gctctcatga tcttgcccgg agtgcggtca ccccaatata tcttatcata ttcagcttcg
     9181 tatccaaggc caaataccac ccggcttacc cctgtggcaa cagtgctgat accggtaccg
     9241 tcggggtttg cagatttgat atggatatct cccgaagcca cccactggtc aaaatagatc
     9301 cggctgccga taactatggt agatcctccc tcaaccagcg gaattatttc agtctcacct
     9361 gtcccgtcga ggttctttga ccacagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnncagg tattcctgtc acttccgaat tcaatatgac gatctcctcc gattgttgct
     9541 ttatctccat cgagacatcg gttttgatca tcagcgatgc cgtaaagacc cctgatgcgg
     9601 aatacagatg ctccgggctc acatcggttg acctggtacc gtcgccgaaa tcccagtaat
     9661 aaaatggatt tccggcctcc gccgggacga tggactcatt ggtgaaattt accgtggccg
     9721 gagcataccc gttgttgtca accgtgtagg aaaagcgggg aactgtgctc gcggggggaa
     9781 cgggaaagcc ctcgtccttg cagctgttga gcagcaggga aagtgacagc actataacaa
     9841 tccgacttac aaacttcatt ttcatatttt cattttttat tggatcagat cacctgttgt
     9901 ggtctgaccc gtcgacactt cagtcagtaa tacagccagg caggctatgc ggaccggcga
     9961 cgccggtccg catagggcca cccgcttttc tatttgacca gcacaatgcc cctcgggctt
    10021 gagagccctg tagccagggt aacctttcct gtaccgttga ggttggcttc gtaaatctct
    10081 cccagacccc tgtcggacca gtacagcttg tccttctcat aatcaatggc aatgccgaaa
    10141 acacggtcat caacatcagc aattgtcaca acaccggaac cgtcaagatt ggccctcttc
    10201 agtgtcatcg aattctggtc atcaaagtaa atcttgttgt tctcggtgtc cacaaataat
    10261 gaatatccgt atacgcctga gaccaggatt gtgggattgg tgccgtcaag gttggcaaca
    10321 tacagattct caaaccaagc ctcataaaag tacagtttgt cgttcttctc gtcaatggca
    10381 accgccctca tactcgcttc ctctattgtc accagttcag tctgaaggct tgaaccatcg
    10441 aggtttgccc tggagatgtg ctcggcgtca tcagcccagt aaaccttgtt attgtcatga
    10501 tctatggcaa caccgtaggg atcaaggata ccggttacta ttgatgcaag tccggtaccg
    10561 tcaggcttca ttttccagat cttgccgtca ggcgtgtcag catcataata atcactgaaa
    10621 aagagatagc ctgtttcatc gtcataggca atgccgggcc ctgacattcc tgccacatcc
    10681 ttgacagttg agagagtatt ggttccgtcg agaactattt t
//
LOCUS       DAOPXU010000096        10497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_7348_length_10497_cov_11.000479, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000096 DAOPXU010000000
VERSION     DAOPXU010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10497
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7348_length_10497_cov_11.000479"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..1739
                     /locus_tag="PKK03_09475"
     CDS             189..1739
                     /locus_tag="PKK03_09475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNX84671.1"
                     /translation="MNMRKFTGLIVLMVVSVVAIVWVQVTWISNSVKAQNEQFDFFVI
                     NSLRNTAHSMETNRRMNFLNEMFIRGYSALPQTIERTASQGLTTGRYSIQSQITSDSD
                     SVEIIVSENENPPVRMKVTRQEASEKMGSSIVVGSDEYMRWMQQQAIEFQSMSNQLAS
                     EMFVWERNKEINKEELLQTLKGELAASGINTPFEFAVIRSDSIIDGVFDKAKRRDFLD
                     SPYRVSLFTQGLFRRGESLSVVFPRKTNYVLGTMGLMLGGFGLFLLIILSTFALSLWF
                     IIRQKKTSEMQADFINNMTHEFKTPIATISLAADTIANSRVITDENRVRHFISMIKKE
                     NSRMNRQVESILQIATLDKHEMDFRFAPTDLHEVIRQAIDTIEIQVTDRGGVVHRHLD
                     AENSIINGDTEHLRNLVHNLLDNANKYSEKAPDITVNTSSNDSGIFLQVADRGIGMSK
                     SVQNRVFERFYRETTGNIHNVKGFGLGLNYVKAITEAHGGTVTVESEQGEGSVFTVFL
                     PFSNETRA"
     gene            1736..2428
                     /locus_tag="PKK03_09480"
     CDS             1736..2428
                     /locus_tag="PKK03_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX84672.1"
                     /translation="MSRKNARIFLVEDDISFGSVLKSYLEINDFEVDLVDDGKAAVET
                     FRKGVYDLCILDVMLPHVDGFTIASEIRIMNSVIPIIFLTAKKLKEDLLKGYGVGADD
                     YITKPFDTDILLCKIRAIIARRDIQDGTRDIYEIGKYIFNSRLRTLTLGDQERQLSPK
                     EGQLLEMLAISPNALVSREAALRKIWGADDYFTARSMDVYVTKLRKYLSDDPQILLKN
                     IHGSGFQLVVGE"
     gene            complement(2544..3029)
                     /locus_tag="PKK03_09485"
     CDS             complement(2544..3029)
                     /locus_tag="PKK03_09485"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNX84673.1"
                     /translation="MNKRETTISFLEYNSEDELPAMEKALVGEAVRAASGAYSPYSHF
                     MVGAALLLDSGEIVRGANVENAAYPSGSCAEKTALSYSVANFSDKKVVAIAIAALTDG
                     SLTEEPVPPCGNCRQMLIEEEQRSSSPIKVILAGKSAIYVLENSQGLMPLSFTGRNLG
                     K"
     gene            3139..>3416
                     /locus_tag="PKK03_09490"
     CDS             3139..>3416
                     /locus_tag="PKK03_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HNX84674.1"
                     /translation="MKNLFLYIVLVSVLLFSSCSPARRAARSTVSTGPASEYAREYIL
                     NYSNLAVSEMKRSGVPASITLAQGMLESNYGRSRLATQGNNHFGIKCH"
     assembly_gap    3417..3516
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3517..3905
                     /locus_tag="PKK03_09495"
     CDS             <3517..3905
                     /locus_tag="PKK03_09495"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNX84675.1"
                     /translation="TVREEEEPIKVISISTGEKTLENNNVEYIIVGEGDTYELLAERH
                     QLLSWEITRYNDLAAGAPLIPGQVVYLQPKRTRAAQGLSVHTVEAGETMYSISQKYAI
                     KLSSLYKMNLMDEGTECRPGQKLRLR"
     gene            complement(3912..4871)
                     /gene="fmt"
                     /locus_tag="PKK03_09500"
     CDS             complement(3912..4871)
                     /gene="fmt"
                     /locus_tag="PKK03_09500"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNX84676.1"
                     /translation="MTESPLRIVFMGTPEFAVASLKALHETGVDIAAVVTVPDRPAGR
                     GRKITFSPVKEYALEHRLALFQPEKLRDPEFISALRDINADLFVVVAFRMLPKEAWSM
                     PPLGTFNLHASLLPQYRGAAPINHAIINGETRTGVTTFLIDKEIDTGMILLSRETGIS
                     PDETAGSLHDRLMVTGASLVVETARGLAARSLKPVPQKKTHDTKLKAAPKIFPADTVI
                     RWEEKAEKIHNLVRGLSPCPGATTMLRKESAGLRLKILGSTISNDITGNIPGTLIVKE
                     KSRLMVACGQGTLEILSLVPEGRKKMSAAEFLRGFDPSGWEMD"
     gene            complement(4864..6108)
                     /locus_tag="PKK03_09505"
     CDS             complement(4864..6108)
                     /locus_tag="PKK03_09505"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNX84677.1"
                     /translation="MTDETKLNTINEAIGEIREGRVVIVVDDEDRENEGDFICAAELI
                     TPEIVNFMATHGRGLICVPLPEERCEELELNMMVGHNTSLHETPFTVSVDLLSPETTT
                     GISAKDRALTIKALVDPSTKPYQLGRPGHIFPLKARTKGVLRRAGHTEAAVDLTRLAG
                     LNPGGALVEIMNEDGTMARLPDLIKLSDKFGIRIISIRDLIRYRLGIESIVEMGEQVK
                     LPTEYGDFDFIPFRQVSNGLEHAALVKGHWTKDEPVLVRVHSSCFTGDIFASKRCDCG
                     PQLHMAMQMVEAEGKGAIIYLSQEGRGIGLINKIRTYKLQEQGRDTVEANIELGFRED
                     ERDYGIGANIMRGIGLGKVRLISNNPVKRAGLEGYGISIVETIPLIIPPNQYNSFYLE
                     TKATKMGHSITCYKTPTKGEND"
     gene            complement(6110..7276)
                     /locus_tag="PKK03_09510"
     CDS             complement(6110..7276)
                     /locus_tag="PKK03_09510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX84678.1"
                     /translation="MAAETILVITAALLAWSYLIYPAAVILAANVKKRRAPSPAVVSA
                     SLPTVTVVMSLHNEKEVAEKKIRSMLASEYPAEKLDFIIGSDGSDDGTGAVITAVAAT
                     NPHIQLIINAERKGKAAMLNELASKAKGDILVVTDANVMFTPSVVRQMADGFADPRTG
                     LCDATVRTLSAADMGITRQEIMYSRFETALKRAEGNLWGAMPGPYGGCYAVRRSLFPR
                     LPDNTLVDDLFVGLSVLKQNYRAFNIAEAVVYEDTQPDMIEQFRRRTRIAAGSFQNLF
                     RFGPFISGRPGVVFAFLSHKIMRWLTPLLLVLFFMTTVILSGRSDFYFCLTAIQLIFI
                     ILSALDIALDRRGKKLLFQRYVTQFLLMNAALTAGFVKVVIGIENGIWEPTKRV"
     gene            complement(7266..8477)
                     /locus_tag="PKK03_09515"
     CDS             complement(7266..8477)
                     /locus_tag="PKK03_09515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX84679.1"
                     /translation="MMKILVLAPRPLWPVHDGGTVASVHCVRGLSATGADVTVLSMVT
                     EKHLTSGISAFGAKPDYLIDYFTVPVNTAIRPLPMILNLLFSGEPYDLARFRSAEYSK
                     TLRSILTGSHFDIIHCEGLPLTLYLNEIRNLTTSPVVLRAHNLEHTIREMMAATACSP
                     LRKYYLSVLSRRLKKTETKAAGQFDAVVPISESDAAWFRSVAGNKPVFLSETGTNEAL
                     MLPEPPPESPRVGFIGAMNWQPNADGINWFISEVWPGVIRKIPSATLHIAGKGLSKGA
                     FLPSGHNIFNEGEPDNALEFIASNHVMVAPLFAGSGIRIKIIEAMSTGRPVVATPAAV
                     AGLPVENGRELAVASDPASFTAAIIRMLENPGLRASTGRAALSLVKERYDNASVTARL
                     LEFYKKLKYGS"
     gene            complement(8449..9948)
                     /locus_tag="PKK03_09520"
     CDS             complement(8449..9948)
                     /locus_tag="PKK03_09520"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNX84680.1"
                     /translation="MKKIDLFMIRSFIGPLVLTFFIVLIIFILQFLWMYVDDLAGKGL
                     ELKVLGELLFQFSLTFVPLSLPLAILLASLMTFGNLGEFLELTALKSSGVPLQRIMMP
                     IFVFSSLLAIGSFLFADYILPISTRKARTLLTDIRRMRPELNIQTGTFYNGIDGFSIK
                     ITDKDPETNRLDGIYVYDHRDGIGNTAVIYADSGYMNVTADESGLILTLYNGHVYKDI
                     EEKNVPQEKRKYPFRRDRFTEQTMVVELEGFGLNRSGMDIYRSNYAMLNTADLTYFID
                     SLSTRFRSRTDAYYSEFNQTRLLTTSHFSLPGVKHVEDSLAPWKTQKFNARAAFDTLS
                     YNDRGTTISRALSFAREGTSFMIEKSEALVAEAKNLRKYEAERYKRFTLPFACLVFFF
                     IGAPLGAIIRKGGLGTPAVISVLFFVIYYVITLSGEKFAKELIISIPLGMLASTIILL
                     PIGAFLTYKATTDAAIMNAETYILFFRKIGAFLTGIKPEKDDEDTGTGA"
     gene            complement(10003..10389)
                     /locus_tag="PKK03_09525"
     CDS             complement(10003..10389)
                     /locus_tag="PKK03_09525"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HNX84681.1"
                     /translation="MRARFEIEYTLNTPVRVLYDRLSTPEGLSEWFADNVTVDGDVFT
                     FHWDKTEARARLIYARENKAVRFDWLDFADTESNFFEFRIIIHELTNDTALIITDHAE
                     EDEIEDARDLWDTQISYLKKVLGVTE"
BASE COUNT         2485 a         2534 c         2733 g         2645 t
ORIGIN      
        1 gttccggcaa agatggctgc cactattcct ataaccgttg agagtgcttt tgtcattgcc
       61 tgtgtttatt ctttctgaca gtacaaaaat agcccatata atccggggag gggttaaagt
      121 gaggttaaaa ctggttaaca aaaagttaaa acggatttcg acattattta tatgtataat
      181 tttgcgccat gaacatgagg aaattcaccg gtctgatagt gctgatggtg gtatctgtgg
      241 ttgctatcgt atgggttcag gtgacatgga tcagcaattc ggtcaaggcc cagaacgagc
      301 agtttgattt cttcgtgatc aacagtcttc gtaacaccgc gcactccatg gagacaaacc
      361 gaagaatgaa tttcctcaat gagatgttca tcaggggata ttcggcactt cctcagacaa
      421 ttgagcgaac agcttcacag ggtctcacga ccggaaggta ctccatccag agccagatca
      481 cctccgacag tgattccgtg gaaataatcg tctctgaaaa tgagaacccg ccggtcagga
      541 tgaaggttac ccggcaggag gccagtgaaa agatgggcag cagcattgtt gtgggatctg
      601 atgaatatat gcgttggatg cagcagcagg caatcgagtt ccagtcgatg agcaaccagc
      661 tggcatccga gatgttcgtc tgggaaagaa acaaggaaat aaacaaggaa gagttgcttc
      721 agaccctgaa gggtgagctg gctgcctcag gcatcaatac gccgtttgag tttgcagtca
      781 tccggagtga ctccataatc gacggagtct ttgacaaggc aaaaagaaga gattttctcg
      841 atagcccgta cagggtcagc ctgtttaccc agggattgtt cagaagagga gaatcgcttt
      901 ccgtggtctt tcccaggaag actaactatg tgcttggaac catggggctt atgcttggcg
      961 gatttggtct ttttctcctg ataatcctct caacctttgc tctgagtcta tggttcatta
     1021 tcaggcaaaa gaagacctca gaaatgcagg ctgacttcat caacaacatg acgcatgagt
     1081 ttaaaacgcc gatcgccact atctcgctgg cagcggatac cattgcaaac agccgggtta
     1141 taactgatga gaacagggtt cgtcatttca tcagcatgat caagaaggag aactcccgga
     1201 tgaacaggca ggtggagtct attcttcaga tagcaaccct tgacaagcat gagatggatt
     1261 tcagatttgc cccgaccgat ttgcatgaag tgatccgcca ggccatcgac accattgaga
     1321 tccaggtgac cgacagggga ggtgtggttc accggcatct cgatgcggaa aacagcatta
     1381 ttaacggtga caccgaacac ctgcgcaacc tggtgcataa cctgcttgac aatgccaaca
     1441 aatactccga gaaagcgcct gacatcacag tgaatacaag cagcaatgac agcggaattt
     1501 tccttcaggt ggctgacagg ggcataggga tgagcaaatc agtgcagaac cgggtcttcg
     1561 aacgctttta ccgtgaaact acaggcaata ttcacaatgt caagggcttt gggctgggac
     1621 tcaactatgt aaaagccatt accgaagctc acgggggaac agtcaccgta gaaagcgaac
     1681 agggtgaagg gagtgttttt acagtatttt taccattcag caatgagacc agggcatgag
     1741 caggaaaaac gcaaggatat ttctggttga ggatgatatc agtttcggat cggtgctgaa
     1801 atcatatctc gagataaatg atttcgaggt agacctcgtc gatgacggga aagcggcagt
     1861 ggagacattc cgtaagggag tttatgatct ctgcatactt gacgttatgc tgccgcatgt
     1921 ggatggtttc acaattgcct cggagatcag gatcatgaat tcggtcatcc ccattatctt
     1981 ccttaccgct aagaagctta aggaggacct gttaaagggt tatggcgtag gggctgacga
     2041 ttatattaca aaaccgtttg acactgatat cctgctgtgc aagatcaggg ctatcattgc
     2101 gaggagggac attcaggacg gaacgcgcga catatatgag atcggtaaat atattttcaa
     2161 ttcgcggttg cgcacactta ccctgggcga ccaggagcgg cagctgtcgc ccaaggaggg
     2221 acagttgctt gaaatgcttg ccatttcacc caatgccctg gtcagcaggg aggcagccct
     2281 gaggaagata tggggcgccg atgattattt cactgcccgg agcatggatg tttatgtcac
     2341 caagctcagg aaatacctga gtgatgatcc gcagatcctc ctgaagaaca ttcatggaag
     2401 cgggtttcaa ctggttgtag gagaataaaa gtctgaaggt ctgaaagtca aaaggtctga
     2461 aggtcagacg tctatgatct tgctgtcttg cagtcctgca gtttgtgagt gaaaatcgac
     2521 aatcgcaaat ctcaaatggc aaattatttc ccaaggtttc tcccggtgaa actgagtggc
     2581 atcagcccct ggctgttttc aaggacataa atggctgatt ttcctgccag gatgaccttt
     2641 atcgggctac tgctcctctg ttcttcctca atcagcattt gccggcagtt gccgcaaggc
     2701 ggtaccggtt cctcagtcag cgagccatca gtcagggcag cgattgctat cgccaccact
     2761 ttcttatcac tgaaattggc aaccgaatat gacagtgcgg ttttctctgc gcaacttcct
     2821 gaggggtagg cggcattttc aacattggct cccctcacaa tctctccgct gtcaagaagc
     2881 agggctgctc caaccatgaa atgcgaatag ggagaataag ctcccgatgc tgccctgaca
     2941 gcctctccaa caagcgcctt ttccattgcc ggcaactcat cctcgctgtt gtattcaagg
     3001 aaagagatag ttgtttctct cttattcata gacattatca gtgaattaca gtgccataaa
     3061 tgtaataata ttttgacctc ctgaggcaca atttgtcagg gctgccgaca ttttcttatt
     3121 tttgccaaaa ggcttcggat gaaaaacctg tttttatata tagttcttgt atctgtcctg
     3181 ctgtttagtt cctgcagccc ggcaagaagg gcagccagga gcacggtctc cacaggtccg
     3241 gcctcggaat atgcacggga gtatatcctc aattacagca atctggctgt aagcgagatg
     3301 aaacgatccg gagtacctgc cagcatcacg ctggcccagg gaatgctgga gtcgaactac
     3361 ggacgcagcc ggctggccac gcagggcaac aaccattttg gcatcaagtg tcacagnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncaac cgtaagggaa gaagaggagc
     3541 cgatcaaggt gatcagcatc tcaactggag agaagacgct cgagaacaac aatgtggaat
     3601 acatcattgt cggagaggga gatacctatg aattgctagc tgaacggcat cagcttcttt
     3661 catgggagat aacccgctac aacgacctcg cagcaggcgc gccgctgata cccggacagg
     3721 tggtgtatct tcagcccaag cgcaccaggg cggcccaggg attatcggtt catacggtgg
     3781 aagctggtga gaccatgtac agcatctcac agaaatatgc cattaaactt tcgtcgctgt
     3841 ataagatgaa cctgatggat gagggtaccg aatgccgccc ggggcagaag ctcaggttaa
     3901 gatagccaat gtcagtccat ttcccatcct gacggatcga agccccgaag gaactccgca
     3961 gcgctcatct tctttcgtcc ctcagggacg agtgaaagta tctcgagggt accctgaccg
     4021 caggcgacca tgagcctcga tttctccttc acaataagcg ttccgggtat gttgccggtg
     4081 atgtcattgc tgatggtgct gcccagtatt ttcaatctga gacctgcgct ctccttcctg
     4141 agcatggtgg ttgcacccgg gcagggtgaa aggcctctta ccaggttatg aatcttttct
     4201 gccttttctt cccatctgat aacagtatcg gcaggaaata tctttggtgc cgctttcagc
     4261 tttgtgtcat gtgtcttttt ctggggtacc ggtttgagac ttctggcagc caatcccctg
     4321 gcagtttcaa ccacaagtga ggcgcccgtc accatgaggc ggtcatggag gctgcctgcg
     4381 gtctcgtcag gtgatatccc tgtctccctc gagagcagga tcatgcccgt atctatctct
     4441 ttgtcaatga ggaatgtggt aacgcctgtt ctggtctcgc cgttgatgat cgcatggttt
     4501 attggtgcgg cgcccctgta ctgcggaagc agagaggcat gaaggttgaa tgtcccgagc
     4561 ggaggcatgc tccaagcttc ctttggaagc atccggaagg ccaccaccac aaagagatca
     4621 gcattaatat ccctcagggc ggaaatgaat tccggatcac gcagtttctc aggctgaaag
     4681 agtgcaagcc tatgttccag ggcatactct ttcacagggc tgaaggtgat cttgcggccg
     4741 cgaccggcag gcctgtccgg aacagtgacc actgcagcga tgtcaacccc cgtttcatgc
     4801 agggctttca ggctcgcaac ggcaaactcc ggcgtaccca tgaatactat gcgcagtggt
     4861 gactcagtca ttttcgcctt ttgttggcgt cttatagcat gtaatgctgt gccccatctt
     4921 ggtggccttg gtctccaggt aaaacgagtt gtactggttg ggaggaatga tcaggggtat
     4981 ggtctcgaca attgagatac cgtatccttc cagtcctgcc cgctttaccg ggttgttcga
     5041 gataagcctg acttttccaa gccctatgcc cctcatgata ttggctccga taccgtagtc
     5101 gcgttcatct tcacggaagc ccagttcgat atttgcctca accgtgtcgc gtccctgctc
     5161 ctgcagtttg tatgtccgta ttttgttaat caggccgata ccccttcctt cctggctgag
     5221 atatataatt gcccctttcc cttcggcttc gaccatctgc atggccatat gaagctgcgg
     5281 cccgcagtca cacctcttcg atgcaaagat gtcaccggtg aagcatgagg aatgcacgcg
     5341 caccaggaca ggttcatcct tggtccagtg cccttttacc agggcggcat gttcaaggcc
     5401 gttactgacc tgcctgaatg ggatgaaatc gaagtcgcca tactcggtag gcagtttgac
     5461 ctgttctccc atctccacta tcgattcaat tccgaggcgg taccgtataa ggtccctgat
     5521 tgaaattatc ctgatgccga acttgtcaga cagtttgatc aggtcaggca gacgtgccat
     5581 ggttccgtcc tcgttcatta tctccaccag tgcaccgccc ggattcagcc ctgcgagcct
     5641 ggtcaggtca acggcagcct ctgtgtgccc tgctctccgg agaacgcctt tggtacgggc
     5701 cttgaggggg aaaatatgcc cgggccttcc aagctggtag ggttttgtgg atggatctac
     5761 cagcgccttg atagtaagtg ccctgtcttt ggccgatatg cctgtagttg tctcgggact
     5821 gagcaggtcg acagacactg tgaacggtgt ttcgtgaaga gatgtattgt ggcctaccat
     5881 catgttaagc tccagttcct cgcatctctc ttcgggtagt ggcacgcaga tcagaccgcg
     5941 tccgtgcgtt gccatgaaat tcactatctc cggtgtgatc agttcagccg cacagatgaa
     6001 atcaccttcg ttctccctgt cttcatcatc cacgacaata accacccggc cttccctgat
     6061 ctccccgatg gcttcattga ttgtattcag ctttgtctca tctgtcattt taaacacgtt
     6121 tggtgggttc ccatatcccg ttttcaatcc ctattactac cttcacaaac cctgctgtca
     6181 gcgctgcatt cattaacagg aactgagtca cataacgctg aaagagcagc ttttttcccc
     6241 tcctgtcaag cgcgatgtca agcgctgaca aaattatgaa aatcagctga attgctgtaa
     6301 gacaaaaata gaaatcagac cggcctgaaa gaatgaccgt tgtcatgaaa aacagcacca
     6361 gcagcagggg ggttaaccac ctcattatct tatgtgaaag aaaagcaaag acaacccccg
     6421 gtcttcctga gatgaagggt ccgaatcgga acaggttctg gaatgatccg gcagcaatcc
     6481 ttgtccgccg gcggaactgc tcaatcatgt caggctgggt gtcttcataa accactgctt
     6541 ccgcgatgtt gaaagccctg tagttctgtt tcagcacgga caggcccaca aagaggtcat
     6601 caaccagggt gttatccggc agccggggga agaggctccg gcgcaccgca tagcagcctc
     6661 cgtaaggccc gggcattgct ccccagagat tgccctcagc tctcttcagt gctgtttcaa
     6721 aacggctgta catgatctcc tgccgcgtta ttcccatgtc agcagcagat aaagtacgga
     6781 cggtggcatc acataatccg gtacgcgggt ccgcaaatcc atctgccatc tgtctgacga
     6841 cggaaggagt gaacatcaca ttggcatcag ttacaaccag gatatctcct tttgccttcg
     6901 aggcgagttc gttgagcatg gctgcctttc cttttctttc agcgttgatg atgagctgaa
     6961 tatgcgggtt agtggctgcc acagcggtta taacggcgcc ggtgccatcg tcagaaccgt
     7021 ctgaaccaat aatgaagtcc agcttttcgg ccggatattc cgaggccagc atgctgcgaa
     7081 tctttttttc tgccacttcc ttctcgttat gcagcgacat caccacggtt actgttggaa
     7141 gggatgctga gaccacggcc ggggaaggtg cccgcctctt ctttacgttt gctgccagga
     7201 ttactgctgc cggatagatg agataggacc atgccagcaa tgcagctgtg ataaccagga
     7261 ttgtttcagc tgccatattt cagctttttg tagaattcaa gcagtcgtgc cgtgacggag
     7321 gcgttatcat atctctcctt taccaggctt aaggctgccc tccctgttga ggccctcagg
     7381 ccgggatttt caagcatcct gataatggca gcggtgaatg acgccgggtc tgaggcaact
     7441 gccagttccc ggccgttttc cacgggcagt ccggccacag ccgcaggggt ggctaccaca
     7501 ggccttcctg tgctcatggc ctcaatgatt tttatcctga tgccgcttcc tgcaaagagc
     7561 ggggctacca tcacatggtt tgaagctatg aattccaggg cgttgtccgg ttctccctcg
     7621 ttaaaaatat tgtgcccgga gggcaggaag gcccccttgc taagtccttt tccggcaata
     7681 tgcagggtgg ctgaagggat ctttcttatg actccgggcc acacttcgga aatgaaccaa
     7741 tttattccgt ctgcattggg ttgccagttc atcgcaccga tgaatccaac gcgcgggctt
     7801 tccggtggag gctccgggag cataagagct tcgtttgttc cggtctccga aaggaacacc
     7861 ggtttatttc cggccactga tctgaaccag gcggcgtcag actcgctgat gggcaccacg
     7921 gcgtcaaact gccccgctgc ctttgtttct gtcttcttca gcctccgtga gaggacggac
     7981 aggtaatatt tacggagtgg actgcaggct gtggcggcca tcatttccct gatcgtatgt
     8041 tcgaggttat gagccctgag gaccacaggg gaggtggtca ggtttctgat ctcgttcagg
     8101 tagagggtaa ggggcaggcc ttcacaatgg attatgtcga aatgacttcc ggtgagtatg
     8161 ctgcgaagtg ttttggagta ttccgctgac ctgaaccttg cgaggtcata aggttcaccg
     8221 gagaagagca ggttcaggat catcgggaga ggcctgatcg ctgtatttac cggaacagtg
     8281 aagtaatcga tgagataatc cggtttcgcg ccgaaggcgg aaattccgga agtcaggtgc
     8341 ttttctgtca ccattgaaag caccgtcacg tctgctccgg tggcagagag gcctctgaca
     8401 cagtgaactg atgccaccgt tccgccgtca tgcaccggcc aaaggggtct aggcgccagt
     8461 accagtatct tcatcatcct tttcgggctt aatgccggta aggaaggctc ctatcttcct
     8521 gaagaagagg atataagttt cggcattcat tatggccgca tcggtagtgg ccttataggt
     8581 caggaatgcg cctatcggga gaaggataat agtggaggca agcatgccca ggggtatgct
     8641 tataatcagt tccttggcaa acttctcacc ggagagggtg atcacatagt aaattacgaa
     8701 gaagagtacg gagatcactg caggggttcc taggccgcct ttccggatga tagctcccag
     8761 gggtgccccg ataaagaaga aaaccaggca ggcaaaggga agggtaaatc tcttgtacct
     8821 ttcagcttcg tatttgcgga ggttttttgc ttccgccacg agagcctcgc tcttttcgat
     8881 catgaatgag gtgccctccc gggcgaagct cagtgctctg ctgatggtgg tgcccctgtc
     8941 gttgtagctc agggtgtcga aagcagcccg agcattgaac ttctgtgtct tccatggagc
     9001 aagggaatct tctacgtgtt ttacaccagg taagctgaaa tgtgaggttg tgaggagcct
     9061 tgtctggttg aactccgagt agtaggcatc ggtccttgac ctgaacctgg tggagagtga
     9121 gtcaataaag tatgtaaggt cagcggtatt cagcatcgcg tagttggagc ggtagatatc
     9181 catccctgac cggttcaggc cgaaaccttc cagttccacc accattgtct gttcggtgaa
     9241 cctgtcacgt ctgaaggggt attttcgttt ctcctgcggg acatttttct cttctatatc
     9301 cttgtataca tgtccgttat acagcgtcag tatgaggccg ctttcgtcgg ctgtcacatt
     9361 catgtaaccg ctgtctgcat aaatgaccgc agtgtttcct attccgtccc tgtggtcata
     9421 gacgtagatc ccgtcgaggc ggttagtttc ggggtccttg tcggttatct tgatgctgaa
     9481 gccgtcgatg ccattataga aggttcctgt ctggatattg agttctggcc gcatacggcg
     9541 aatgtcagtc agcagtgtcc ttgctttccg tgttgagatg ggaaggatgt aatcagcgaa
     9601 caggaatgat ccgattgcga ggagggagga gaagacaaag ataggcatca tgattctctg
     9661 gagaggcacg ccggaagatt taagggctgt cagttcgagg aactcgccga ggttgccgaa
     9721 agtcatcaga gaggcaagga gtattgccag cgggagtgac aggggaacga atgtaagtga
     9781 gaactggaag agtagttcgc cgaggacttt gagttcaagg ccttttccgg cgaggtcatc
     9841 gacatacatc cagaggaact ggagaatgaa gataatgagc acgatgaaga atgtcaggac
     9901 cagcggtccg ataaacgatc ttatcataaa cagatcgatc ttcttcattg tctcgggtgt
     9961 ggtgcaaacg tacgttattt ttgagaaaga tgcaaagctc tctcactcgg tgacgccgag
    10021 gacttttttc aggtatgaga tctgggtgtc ccagaggtct ctggcatctt cgatttcatc
    10081 ctcttctgca tggtcggtaa tgataagtgc agtatcattg gtgagttcat ggataattat
    10141 tctgaattcg aagaagtttg attcggtgtc ggcgaagtcg agccagtcga atctgacggc
    10201 tttattttcc cttgcgtaga tgagccgggc tcttgcctct gtcttatccc agtggaaggt
    10261 gaagacatcg ccgtcgacag tgacattgtc agcgaaccat tctgacagtc cttccggggt
    10321 gctcagcctg tcgtaaagaa ctcttaccgg tgtattcagg gtgtattcaa tttcaaatct
    10381 ggctctcatt tcagggaagg actgtcatgc aatataaaaa taatagatga aaagaaaaag
    10441 tttgttcggc aaaatgaaaa aatatttata tttgcagcct tgaaaaaccc gcctttt
//
LOCUS       DAOPXU010000097        10463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_7377_length_10463_cov_11.281130, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000097 DAOPXU010000000
VERSION     DAOPXU010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10463
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7377_length_10463_cov_11.281130"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2127
                     /locus_tag="PKK03_09530"
     CDS             <1..2127
                     /locus_tag="PKK03_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNX84682.1"
                     /translation="EKQVHNYPHCWRTDKPVLYYPLDSWFIRTTALRDRMIELNNTIN
                     WKPESTGTGRFGKWLENLVDWNLSRSRYWGTPLPIWMTDDRQEEICIGSLAELKMEIA
                     RSIDAGYMTRNPLENFAEGDFSAENYSLLDLHRPFVDEIVLVSPTGRPMHREPDLIDV
                     WFDSGAMPYAQAHYPFENRENFSEMFPADYIAEGVDQTRGWFFTLHAIATMISDSVSF
                     RNIISNGLVLDKNGNKMSKRLGNAVDPFSTIDTHGADPVRWYMLTNAQPWDNLRFDIS
                     GVEEVKRKFFGTLYNTYSFFALYANVDGFTGHEPQVPVERRPEIDRWIISLLNTLIKE
                     VTARYDDYDITPAGRAVSEFVTENLSNWYVRLNRKRYWGGGMDEDKLSAYQTLYTCLE
                     TVALLASPIIPFYTDRIFTDLNSVRGRFSEGSVHLAPFPSYDEKLIDRDLEERMEIAQ
                     KMSSMILALRRKVNIRVRQPLAKIMVPVTDAGFREKFEAVRPLVLAEVNVKDVEYVDD
                     TSGILVRRIKPNFKLLGPKFGRQMKEAGAAIMALTQDQIVQFERDGFLDLSLDGATGA
                     AGAVGAAGAADPNGNVHRITTEEVEIISEDIPGWLVANEGKLTVALDITVTEELLHEG
                     IAREFVNRIQNIRKESGFEVTDKIRVVIEDLPSVAAAVRKHSDYIASQTLALEVTLAP
                     ASSISGPAARETDIEEQMVKVLVEKV"
     gene            2321..3139
                     /locus_tag="PKK03_09535"
     CDS             2321..3139
                     /locus_tag="PKK03_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX84683.1"
                     /translation="MDDIIKYPVQHPLLKKYIRFFWALRIDHAQLNHKIIPQRNINLR
                     FNLSETSHFVNINNSEYSLEDVYFLGLHDHFGNVNLKINGKVDVLGICFYPDGFYPFL
                     KIPVSEFKNQLLGAEEVGFKAAVSINEQLKLTTDIGLRLNILETGLVKILDDDFYIPR
                     DFRQLFLSLKNGGNFDHLTEFCKQNNVSIRKSERLFNKYVGIPANTYMLLNRFHSSVN
                     QILYSDYSKLSDLAYDNGYFDQMHFISDFKRFAGNTPKKFSQQNNSILQIGKMG"
     gene            3211..3519
                     /locus_tag="PKK03_09540"
     CDS             3211..3519
                     /locus_tag="PKK03_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84684.1"
                     /translation="MKTKVITEFVKMEVLQTTTEEQLLAKTEIINQFLRKQDGFIDAE
                     LVKAIEGNVWFLIYHIENFEKLKALGEKLRAMKLFDEIIPLIEPGSMSVSFYQKIKTW
                     "
     gene            3660..4208
                     /locus_tag="PKK03_09545"
     CDS             3660..4208
                     /locus_tag="PKK03_09545"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HNX84685.1"
                     /translation="MKGLLIIDMQNDSFPEKMPVHDLEGVVLRINKLAESFRKKNYPV
                     IHIQHDGTLQNSCLPGTHGWQLIPSLIKEPKDLLVSKTVNDSFYGTELKNVLESEHID
                     TLVIAGFATDFCVDTTVRSAINKNYKVLIAGDCHTTFSRSFLSAEIIIRHHNLVWGYL
                     IPTKYKAEVKTCDELIAQLQEA"
     gene            4257..4829
                     /locus_tag="PKK03_09550"
     CDS             4257..4829
                     /locus_tag="PKK03_09550"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNX84686.1"
                     /translation="MPETLISEKVMNYVEDACIQPANQLTKSFYREHVLVVHKYCGIL
                     ASVLKCDEDIACISALLHDISVVFDFKSLPNHNSESAGIAERVLRSFKYPEDRIARVK
                     RCISNHMSPLKINEGPLEDVVLSNADAISQIIVPSYWLYFAFKIRNSGYDEGLKWYSD
                     RIENNWDSLIDPAKSLIEERYRNIRLTLHL"
     gene            complement(5203..6357)
                     /locus_tag="PKK03_09555"
     CDS             complement(5203..6357)
                     /locus_tag="PKK03_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013815704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX84687.1"
                     /translation="MITREAETELRILAEQFKAVAVLGPRQSGKTTLVRKVFESKPYV
                     SLENPDMRLFAMDDPKGFISNYPDGAVLDEIQRVPELFSYLQQILDDQTANGFFILTG
                     SNNFLLQESISQSLAGRVAYLFLLPLTISEIGDRESNINSVLFSGCYPALYNNTTDPA
                     RFYANYIRTYVERDVRLIRNITDLYAFEKFLRLCSGRIGQLLNMSSLASEVGVDLKTI
                     GSWIGVLEASFILFRLQPHHMNFNKRIVKMPKLYFYDTGLAAALLGIEKADYLTMHPS
                     RGSLFENMVIVDFLKNRYNRGRLSNLYFWRDNTGNEIDLLIDNGISQIPVEIKSGQTL
                     SEESLRGLRFWSKLTGIEEGYLIYGGGEIQKRSNGITAVPINSLNIIYNL"
     gene            6549..7151
                     /locus_tag="PKK03_09560"
     CDS             6549..7151
                     /locus_tag="PKK03_09560"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNX84688.1"
                     /translation="MFSGIVEEAAPVVRIEQDKDNIHITMRCSFAHELKIDQSVSHNG
                     VCLTVVKKEGDTYTVTAIRETLDKTNLGLLKPGDKVNLERSTRLDGRLDGHMVQGHVD
                     LTATCTSVREAEGSWYYTFEYEPKSPEHITVEKGSVSVNGVSLTVVNSKDRSFDVAII
                     PFTWQVTNFHQIKAGTVVNIEFDILGKYIAKIVRQVLGKA"
     gene            7356..7985
                     /locus_tag="PKK03_09565"
     CDS             7356..7985
                     /locus_tag="PKK03_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HNX84689.1"
                     /translation="MKNRKIIVAITGASGAVYAVKLLERLRSLRAADTLRVAQPKVKA
                     EQSNLLPETLQEVAVILTGNAEDIMLYETGAGYTPSGNEKLWSNKDFNAPFASGSSSY
                     DTMIICPASMGMVGRIAHGVSDDLITRAADMMLKERRRLILVPRETPYSLIHINNMKL
                     LTEAGAIICPATPSFYSRPQTIDDLVMTVIERVLTLAGFTIDAYRWNEE"
     gene            8072..8536
                     /locus_tag="PKK03_09570"
     CDS             8072..8536
                     /locus_tag="PKK03_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNX84690.1"
                     /translation="MEQPALHKRIRSLFRSHHLLSIATVSEKGPWCASCFYARDEENN
                     TLVITTDPATRHGAEFRANPQVAGTIALETKRIGRIRGAQFTGTISEPTGDDLSRART
                     VYLKRFPYAALTEIHLWVIKLNQIKLTDNRLGFGKKIIWTSGDDFVESAGQI"
     gene            complement(8697..9890)
                     /locus_tag="PKK03_09575"
     CDS             complement(8697..9890)
                     /locus_tag="PKK03_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX84691.1"
                     /translation="MEQLFERYYRLLDSVSVDFTRGYAEKINWNARMIGVRGARGIGK
                     TTILLQHMKRNFKRDGSALYVSLDNIWFSENRLIELADVFIKQGGRYLFLDEVHKYNG
                     WSQELKNIYDGYPDLKIVFTGSSLLEIRKSRADLSRRAVVYNLRGLSFREYLNLMVGL
                     EMPVFSLDDILSGHVDHAQEVLKQVKPLKYFASYLKSGYYPFFLEVPELYFHRLDEII
                     NLILENELPLLRNVELANVPKLKLLLKILAESAPFVPNIVKLSERSGLTRNTVISYLG
                     YFEEAHLGKNLYKNAKGITRLQKPDKIYLENTNLMHALSPQGVNSVNLRETFFVNQLS
                     ENHIVEYASEGDFIIDDKFIIETGGKSKGFYQIKGILNSYIASDGIEYGTGNRIPLWL
                     FGFLY"
     gene            complement(9968..10333)
                     /locus_tag="PKK03_09580"
     CDS             complement(9968..10333)
                     /locus_tag="PKK03_09580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNX84692.1"
                     /translation="MGTIKDFEDLEIWRLSRELVNLIYSDFRLCRDFTFRNQVTGAGI
                     SIMNNISEGFFRNSDTEFRHFLNIAKGSSGEVKSMYYIAEDQNYVSAELALDRRNRCQ
                     RIMAGVTTLMKYLRTTQKP"
BASE COUNT         2928 a         2464 c         2389 g         2682 t
ORIGIN      
        1 gagaagcagg tgcacaacta cccgcactgc tggcgcaccg acaaacccgt gctgtactac
       61 ccgctcgact catggttcat ccgcaccacc gctctgcgcg accgcatgat tgagctcaac
      121 aatacaatca actggaagcc tgagagcacc ggcaccggcc gcttcggcaa gtggctcgag
      181 aacctcgtcg actggaacct gtcgcgctcc cgctactggg gcacgccgct gcccatctgg
      241 atgaccgatg accgccagga ggagatatgc atcggttcgc tcgcagagct gaagatggag
      301 atagcccgca gcatcgatgc aggatacatg acccgcaacc cgctggagaa cttcgccgag
      361 ggcgacttct cggctgagaa ctattcgctg cttgacctgc acaggccatt tgtggacgag
      421 attgtgctgg tgagccccac cggcaggccc atgcaccgtg agcctgacct gattgatgtc
      481 tggtttgatt cgggagcgat gccctatgcg caggcgcact acccattcga gaacagggag
      541 aatttttccg aaatgttccc ggctgattat atcgccgagg gcgtcgacca gacacgcggg
      601 tggttcttca cgctgcacgc catagccact atgatctctg attcggtttc gttcaggaac
      661 atcatttcca acgggttggt gctcgacaag aacggaaaca agatgtcgaa acggctcggc
      721 aacgcagttg atcctttttc tacaattgac acgcacggcg ctgatccggt gcgctggtac
      781 atgctcacca acgcccagcc gtgggacaat ctccgctttg acatatcggg cgtggaggag
      841 gtgaagcgca agttcttcgg gacgctttat aatacatatt ccttcttcgc gctttacgcg
      901 aacgttgacg gattcacggg ccatgaaccg caggtgcccg ttgagcgcag gccggagata
      961 gaccgctgga taatctcgct gctcaacacg ctgattaagg aggtgaccgc gaggtatgat
     1021 gattatgaca tcaccccggc aggccgggct gtttccgagt ttgtaacaga aaatctctcg
     1081 aactggtacg tgcgcctcaa ccgcaagcgc tactggggcg gcggcatgga cgaggacaag
     1141 ctctcggcat accagacgct ctatacatgt cttgagactg tggcgctgct ggcctcgccg
     1201 ataataccgt tctatactga ccgcattttc acggatctca attccgtccg tggccggttc
     1261 tctgaaggtt cagtgcacct ggcgccgttc ccgtcatatg atgagaagct gattgacagg
     1321 gatctcgagg agcggatgga gattgcccag aagatgtcgt cgatgatact ggcgctgcgc
     1381 cgcaaggtga acatcagggt gcgccagccg cttgcaaaga tcatggtgcc ggtgaccgat
     1441 gccggcttcc gcgaaaaatt cgaggctgtc aggccgctgg tccttgctga ggtcaatgtc
     1501 aaggacgttg agtacgtgga tgacacctcg ggaatccttg tgcgtcgcat caagcccaac
     1561 ttcaagctgc tgggtccgaa gttcggcagg cagatgaagg aggccggcgc tgcaataatg
     1621 gcactcacgc aggaccaaat agtgcagttc gagcgcgacg gtttcctgga cctttcactt
     1681 gacggagcaa ccggtgcggc cggtgcggtc ggtgctgctg gtgctgcaga cccgaacggt
     1741 aatgtgcacc gcatcaccac cgaagaggtg gagatcatct ccgaggacat acccggatgg
     1801 ctcgtagcca acgagggcaa gctgacagtg gcgcttgaca taaccgtcac cgaggagttg
     1861 ctgcatgagg gcatagcccg cgagtttgtc aaccgtatcc agaacatacg caaggagagc
     1921 ggtttcgagg tgaccgacaa gatcagggtg gtgattgagg atctgccgtc agtggcggcc
     1981 gccgtgcgca agcactcgga ttatattgcc tcgcagacgc tggcactgga ggtcacgctg
     2041 gccccggctt cctccatctc cggccccgct gcccgcgaga cagacatcga ggagcagatg
     2101 gtgaaggtgc tcgtggaaaa agtctgaagg tctgatccgc cagaaggcgg acaatagtct
     2161 gaaggccggt attacggatt ttacccctga tccgacaacc tgctgccggc gggaaaataa
     2221 tcgattgtac caactattta aaaacatgtc ttgttgagaa ccgggtaatt aatgttattg
     2281 ataattttag caggaatatt ggatttgcct ggtctcattg gtggatgata taattaaata
     2341 tccggttcaa catcctttac tgaagaaata tatcagattc ttttgggcgt tgcgcattga
     2401 ccatgcacag ctcaatcata agataattcc tcaaagaaat attaatctga ggtttaacct
     2461 gagcgaaact tctcattttg taaatatcaa caatagcgag tattcgcttg aggatgttta
     2521 ctttttaggt ttgcatgacc atttcgggaa tgtgaacttg aaaataaacg gcaaagttga
     2581 tgtgctggga atctgctttt atcccgatgg attttatcca ttccttaaaa tacctgtttc
     2641 tgaatttaaa aatcaattac tgggagcaga agaagtggga tttaaagcag ccgtcagtat
     2701 caacgagcag ttaaagttaa caactgatat tggattacgg ttaaacatcc ttgaaaccgg
     2761 gctggttaag atacttgacg acgatttcta tattccccgg gacttcaggc agttattcct
     2821 gtcactgaaa aatggcggca attttgatca tcttaccgaa ttttgtaaac agaacaatgt
     2881 cagtatacgt aagtctgaac gcctgtttaa caaatatgtc gggattccgg caaatactta
     2941 catgttgctt aatcgtttcc atagcagtgt caatcaaata ctttacagtg attattccaa
     3001 actgtcagac ctggcatatg acaatggcta ttttgaccag atgcatttca tcagtgattt
     3061 caagcgtttc gcaggaaata cgcccaaaaa attctctcag caaaataatt ctatcttaca
     3121 aatcgggaaa atgggctgag tgtcgttcct gtactatttt gtgatgtcat cagcctttac
     3181 ttttgccaca taattctaaa acaaagcatt atgaagacaa aagttattac tgagtttgtg
     3241 aaaatggaag tattgcaaac tacaactgaa gagcagctcc tggctaagac tgaaatcatt
     3301 aaccaatttc tccggaaaca ggatggtttt attgatgctg aactcgtaaa agctattgaa
     3361 gggaacgttt ggttccttat ctatcacatt gaaaattttg aaaagctgaa agcattggga
     3421 gaaaagttgc gggcgatgaa gttatttgat gaaattatcc ctcttatcga gcccggaagc
     3481 atgagcgtga gtttctatca aaaaattaaa acctggtagt gccggggaaa taatcagagg
     3541 aggccgccag ggaaaataaa atcagcaaga aaccgcctgg cggcctccgg taagtacgac
     3601 cagcacagta agtcaggctg atggccggaa tacatttttg acgattatac tattatcata
     3661 tgaaaggttt gttaataatt gacatgcaaa atgacagttt cccagagaaa atgccagtgc
     3721 atgatctgga aggggtggtc ttaagaatca acaaactggc agagtctttt cgaaagaaaa
     3781 actatccggt aatacacatt caacacgatg gcaccttgca aaattcatgt ttgcccggca
     3841 cacatggatg gcagttgatc ccttcactga taaaagaacc gaaggatctt ttggtcagta
     3901 aaacagtcaa tgattcattt tacgggactg aactgaaaaa tgtactggaa tcggaacata
     3961 ttgatacatt ggtcatagcc ggctttgcaa ctgatttctg tgttgataca acagtaaggt
     4021 cagccataaa taaaaactat aaagtattga ttgcgggcga ttgtcataca acattcagcc
     4081 gctcattttt atctgccgaa ataattataa ggcaccacaa cctggtctgg ggatacctga
     4141 tcccaacaaa atataaggca gaggtcaaaa catgtgatga gttaatagcc caattgcagg
     4201 aagcataaaa gtcgcaaatt gcagtccgga ttttatcatg attaaaaaaa acaaaaatgc
     4261 ctgaaaccct gatctcagaa aaggtaatga attatgtaga ggacgcatgc attcaacctg
     4321 ccaaccaact cacaaaatcc ttttacaggg agcatgtcct cgtcgttcac aagtattgtg
     4381 gtatactcgc ctctgtattg aagtgtgatg aagatattgc ctgcatttca gctctgttgc
     4441 atgacatttc ggtggtgttt gattttaaaa gtttgccaaa tcataattcg gaaagtgcag
     4501 gtatcgcaga aagggtgctt cgttcattta agtaccctga agacaggata gccagagtaa
     4561 aaaggtgtat ttcaaaccac atgtcacctc tgaaaataaa cgagggacca cttgaggatg
     4621 tggtgctttc aaatgctgat gccatttctc agatcattgt tccttcctac tggttgtact
     4681 ttgctttcaa aatccggaat tcaggctatg acgagggcct gaaatggtat tctgacagaa
     4741 ttgaaaacaa ctgggactct ctcattgatc ctgcgaaaag cttaatagaa gaaagatatc
     4801 ggaatatcag gttaacctta cacctgtaag agttactgtt tcagaaccat ggtaaaacta
     4861 tcaggattgc cgggggcaca tttctgtaac tgtagttgtg ataagatccg ttgattcccc
     4921 ttactgtttc caccggacac atcgtcgatt caaatccatc aatcttctga gtatggaggt
     4981 cctcctgccg gttgaaataa catttcctat ctttgcccgg aacaaacagc agacaatatg
     5041 ttttcgggaa tagtagagga agctgcaccg gtggttcgga tagaacagga aagggataac
     5101 attcaaatta caatgcgacg ctcattcgcc aatgagccga agtcgctata gcaaatcacc
     5161 gtggagaaag gttcagtcag ccagggagaa tataactggt cactacaaat tatatatgat
     5221 attcagagaa ttaattggaa ccgcagttat accgttgctc cttttttgaa tttctcctcc
     5281 gccataaata agataacctt cttcaatccc ggttagcttg ctccagaatc ttaagcctct
     5341 taacgactct tccgacagag tctgaccaga tttaatctcg accggtatct gtgatattcc
     5401 gttatcaatc agcaaatcta tctcattgcc tgtattatca cgccaaaaat acagattgga
     5461 taatcgtccc cggttatatc tgtttttcag gaaatctact atcaccatat tttcaaacag
     5521 gcttcccctt gaaggatgca tcgtcagata atcagccttt tcaatcccaa gaagtgcagc
     5581 cgccagtcct gtatcataaa aatataattt aggcatcttc acaattcgct tgttgaaatt
     5641 catgtgatgc ggttgaagcc ggaataagat aaagcttgct tccagaacgc caatccacga
     5701 accgattgtc tttaaatcaa cccctacttc tgatgccagg ctgctcatat tcagcagctg
     5761 accaatccga ccggaacaca atcggagaaa cttttcaaaa gcataaagat ctgtgatatt
     5821 cctgataagc cggacatcgc gctccacata agttctgata taattcgcat aaaaccgggc
     5881 agggtcagtg gtgttattgt ataaagccgg ataacaacca ctgaagagaa cagaatttat
     5941 attactctcc ctgtcaccta tttcgcttat tgttaatgga agcaggaaaa ggtaggcaac
     6001 cctgcctgca agactttgtg aaatgctctc ctgcaacagg aaattattgg atccggtaag
     6061 tataaaaaat ccatttgccg tctgatcatc aaggatttgc tgcaggtagg agaaaagctc
     6121 cggaacacgt tgtatttcat ccagaacagc accatcagga taatttgaga tgaacccttt
     6181 aggatcatcc atggcgaata atcgcatatc aggattctca agactgacgt acggtttgga
     6241 ttcaaatact tttcttacaa gcgtcgtctt tccggactgg cgaggcccca gaactgcaac
     6301 agccttgaat tgctctgcaa gtatccttag ctctgtttct gcttcacggg ttatcataac
     6361 acaaatttac aattctggaa ttgaattcca atattgtaaa aaagaattta taataaagat
     6421 gtcatattta ttttgtcggc gcgatatcgc aggtttcaga agatggctta ccccgtcagt
     6481 acgacactac agtctctgcc tcttctccca acatttccta tctttgcccg gaacaaacag
     6541 cagacaatat gttttcggga atagtagagg aagctgcacc ggtggtgcgg atagaacagg
     6601 ataaggataa tattcacata accatgcgat gctcatttgc ccatgagctg aagatagacc
     6661 agagcgtgtc gcacaatggt gtctgcctta cagtggtaaa gaaggagggt gacacctaca
     6721 cagtcaccgc catccgcgag acactcgaca agaccaatct cgggctcctg aagccgggcg
     6781 acaaggttaa cctggaacgg agcacccgtc ttgacggcag gcttgacgga catatggtcc
     6841 agggccatgt ggatctcacc gcaacatgca catccgtgcg cgaggctgag ggttcatggt
     6901 actacacatt tgaatacgag ccgaagtcgc ccgagcacat caccgtagag aagggctcgg
     6961 tcagcgtgaa cggagtgagc ctcacggttg tcaactcgaa ggaccggtcg tttgatgtgg
     7021 caatcatccc cttcacctgg caggtgacaa acttccacca gataaaggcc ggcacggtag
     7081 tcaacatcga gtttgacatc ctcggcaaat acattgcgaa gattgtcagg caggtgctgg
     7141 ggaaagcctg aaattcataa ttgtctgcac tatcgtcaga cttcagacct tattgtaaga
     7201 gtgtaaaatc tccgtaaaaa ttgattttgt ttctgttttg cactattaat gatgcattac
     7261 agaaataaaa cagatatcat actgacaacc ctaggcctta aacctttaat aattgcgggt
     7321 tgtaacaatt tatatctttg catttccaga gggttatgaa aaaccgaaag ataatcgttg
     7381 ccatcacggg cgccagcggg gcggtatatg ccgtgaagct gctggagcgg cttcgctccc
     7441 tgcgggcagc agatactttg cgggttgcac aaccaaaggt gaaggcagag cagtcgaacc
     7501 tgctaccgga gacgctgcag gaggttgctg tcatcctcac cggaaatgcc gaggacatca
     7561 tgctctacga gaccggcgcc ggttacactc cctcaggcaa cgagaagctc tggagcaaca
     7621 aagatttcaa cgcaccgttc gcctccggat cttcttcata tgacactatg attatctgtc
     7681 ccgcctcgat gggaatggtc ggacgcattg cacacggcgt atccgatgac ctgatcaccc
     7741 gtgcagctga tatgatgctg aaggaaaggc gccgactgat actggttccc cgcgagacac
     7801 cctacagcct catacacatc aacaacatga agctgctcac agaggccggc gcaattatct
     7861 gcccggccac cccgtcattt tacagccggc cgcagacaat tgatgacctg gtaatgacgg
     7921 tgattgagag ggtgctgacg ctggcgggtt tcacaattga tgcttacagg tggaacgagg
     7981 agtagttgtc ttgttcgata cagaaaacct ataaaccaca gttaaacgag gagcagcagt
     8041 aagatgctgc gaagaaaacc aacagatccc ggtggaacaa ccagcacttc ataaacgaat
     8101 cagatcgctt ttcaggagcc atcatttgct gagcatcgcc accgtcagcg aaaagggacc
     8161 gtggtgcgca tcatgcttct atgcccggga tgaggagaac aacacccttg ttatcaccac
     8221 cgaccctgcc acaaggcacg gtgctgaatt ccgggccaac ccgcaggttg caggcaccat
     8281 cgccctggaa acgaaacgca tcggtcgcat ccgcggcgcc cagttcaccg gcaccatcag
     8341 cgagcccacc ggtgatgacc tcagccgcgc cagaaccgta tacctgaagc gcttccccta
     8401 cgccgccctg actgaaatcc acctgtgggt cataaaactc aaccagataa aactcacgga
     8461 taacaggctg gggttcggga agaaaatcat ctggacatca ggtgacgatt ttgtcgagtc
     8521 tgccggccag atataacggc aggggaatga tatttcaata tgtttgcttt ttatataaag
     8581 catttattgt taataattgc tttccaaata aagcaattat tttagtgtct ttccgacttc
     8641 agaaaatatc tcattcgagc tgaacactca gtacattcgg gcagaaagcc ggtaaatcaa
     8701 tataaaaatc caaacagcca taagggaatc ctgtttccgg ttccgtattc aatcccgtct
     8761 gaagctatat agctgttaag gatcccctta atctgataaa atccctttga tttccctcct
     8821 gtttcaataa taaatttatc gtcaattatg aaatcaccct cgcttgcata ctcaacaata
     8881 tggttctcgc ttagctgatt gacaaaaaat gtttcacgaa ggtttacgct gttcactccc
     8941 tggggagaaa gggcatgcat gaggttggtg ttttcaagat agattttatc tggtttctgc
     9001 aaccttgtaa ttcctttcgc gttcttgtac aagtttttgc ccagatgagc ctcttcaaag
     9061 tacccgagat aagaaatgac agtattgcgg gtcaaccccg atctttcact aagcttgacg
     9121 atatttggaa caaagggagc tgattcggca agaatcttta aaagaagctt cagtttgggc
     9181 acattcgcta attcaacatt ccgcagaagc ggcagttcat tttccagaat cagatttata
     9241 atctcgtcaa gcctgtgaaa atacaattcc ggaacttcaa gaaagaaagg atagtagcct
     9301 gatttaaggt aggaggcaaa atatttcagc ggcttgacct gcttcaacac ttcctgtgca
     9361 tgatccacat gtcctgaaag gatatcatca aggctgaaaa caggcatttc cagtccaacc
     9421 atcagattca ggtactctct gaatgaaagc cccctcaaat tatagacaac tgccctgcgg
     9481 ctcaggtcgg ctctggattt gcgtatctca agcagtgatg aaccggtgaa gactatcttt
     9541 aaatccggat aaccgtcata gatattttta agttcctgcg accagccatt atatttatgc
     9601 acttcgtcca gaaagaggta acgcccaccc tgcttgataa acacatctgc cagctcaatc
     9661 agcctgttct ccgaaaacca tatattgtca agactcacat agagggcaga accgtcacgc
     9721 ttaaaatttc ttttcatgtg ctggagcaaa atggttgttt tgccgatgcc acgagcaccc
     9781 ctgaccccta tcatacgggc attccagttt attttttcgg cataacccct ggtaaaatcc
     9841 actgaaacac tgtcaagtaa ccgatagtat ctttcaaata gttgttccat aactgctttt
     9901 tatataaagc aaatatagca atttattgct ttatttaacg agcatcactg gatatttcgt
     9961 ctgaaggtca gggcttttgg gttgttctga gatatttcat gagagtggtg accccagcca
    10021 ttattctctg acatctgttc cttctgtcaa gtgcaagttc agcagataca taattttgat
    10081 cctctgcaat gtagtacatg cttttaactt ctccggaaga acctttggct atattaagaa
    10141 agtgtctgaa ttcagtatca ctgtttctga agaatccttc agagatgtta ttcataattg
    10201 agatccctgc cccggtaacc tggttcctga aagtaaaatc ccggcataac ctgaaatctg
    10261 aataaatcag attaaccaac tctctggata acctccatat ctccagatcc tcaaaatcct
    10321 ttattgttcc catctttaca cgttttagaa atacagcact ttaaagttaa taaattcctg
    10381 tgaccttcag acctcagacc ttcagacctc agaccttcag acctcagacc ttcagacctt
    10441 cagacctcag accttcagac ctc
//
LOCUS       DAOPXU010000098        10185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_7689_length_10185_cov_11.432675, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000098 DAOPXU010000000
VERSION     DAOPXU010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10185
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7689_length_10185_cov_11.432675"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1748)
                     /locus_tag="PKK03_09585"
     CDS             complement(<1..1748)
                     /locus_tag="PKK03_09585"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNX84693.1"
                     /translation="MNHILPFSAIILTAALAMCTSPADDTTPPNEPARLIREENNISL
                     LYNGAAILTLEIDLPQAEYTVKELDEERDCKVSCVITISSLNGSAMNITGRILAGKES
                     FTCEADRRIEGTQVVRHTYGISHSLLNRAVYDRQSDWLLSADQSYTSSSLKIEPETAD
                     STAITYALSMKGNEVTFRFRPRYYQKHRGLDYYEPWNYQVWKEPVAGWCSWFAYKTGI
                     NQKNVMEATDVLAETMKPYGLEYIQIDDGYQQYGGTPEAWINPNDKFPSGLGDLATYI
                     SSKGFRPGIWTNVAVHDSAWAYSHPDWFVRNADGTPASGRWIGYILNGLDDEMLDSVI
                     TPVYSRFRNDGWSYFKLDALRHLLFEGYNSHSSFFSGGYAEREKAFRNVVGRVREETG
                     RNNFLLACWGVLPGVVGLADGCRIGNDGYGYTALAQYNSFNNVVWRNDPDHIELTPQE
                     AWRSCMATSLTGSLFMVTDKPEVYRTGLIDPARKSLPVLFTLPGQVFDLDPSRSAFLD
                     RTTTEMSGSGERESDGSRTSPFDLFMLELNMPWERWCVLGRLGEKEESISLAELGLEK
                     GKEYLLYEFWSDTFLGT"
     gene            1921..4725
                     /locus_tag="PKK03_09590"
     CDS             1921..4725
                     /locus_tag="PKK03_09590"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNX84694.1"
                     /translation="MKNLLRAALILLVVTAVSCQKKAADELSLPFEKYELGNGLDVIL
                     HQDHSDPIVSVAIYYHVGSNREVPGRTGFAHLFEHMMFQQSENVPEDQYFRLIQTAGG
                     TLNGSTNQDRTNYYETVPKNALEMVLWMESDRMGYLTNTITQQAFAIQQNVVQNEKRQ
                     NYDNRPYGHSSTVMAKALFPEGHPYSWTTIGEMKDLFNATVDDVKEFHGRFYIPNNAT
                     LSIAGDFDPAEVKALVQKYFGEIPAGADVEKMSPMPVTLAETKKLYHEDNFANTAQYT
                     MAFPTVEMYNPDAYALTILSDILAGGKKSPMYNVLVKEKKLTSGVGAYNSAQLLAGTF
                     RVSVNANPGVSLTDIENAIFESFARFEAEGFTEKDLEKVKAGQETRFYNMISSIDGKS
                     KQLAEYNMYAGDPGYYRQDMEALKAVTIGDVKKVYEKYIKGKNFVATSFVPKGKVDLA
                     SAGSVNAGIEEENILTATEVKVEAGAAEEIKKTPTSFDRSVQPPLGPDVSVTVPTVWK
                     SSLANGMKLYGIQHTEVPLVQYDIVIEGGHMLDDISAPGVASMLATMLNEGTRNKTPE
                     ELEEEIDLLGASIRVGAGSEDITVSVNTLARNFEKTLAIVEEMLLQPRWDSAAFELAK
                     TRTLNSLRRNSIDPTYLGSRALNNLILGESNIYATDVSGTPVSVQAMTMDQLKAFYEK
                     NFSPSISRFLVVGAVDQPRVEKALASLNTNWAAKEVQMPEVMPAQKPEKSGIWFVNIP
                     DAKQSNIFIGAPAIPRGHPDYYPAYVANYKLGGSFNGYVNLVLREEKGYTYGARTNLS
                     AMKNYGYFNASSAVRSTATLESVEIFRDLMARYREGVSQEDVDFTKNALLKGNALRFE
                     TQRALLGMISTMSEYGLPDDYIAQEENYVRELTVEKVNEMVNKYIDPMKMYYVVAGDA
                     ATQLKELNKLGFGEPVLVKF"
     gene            4731..7532
                     /locus_tag="PKK03_09595"
     CDS             4731..7532
                     /locus_tag="PKK03_09595"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNX84695.1"
                     /translation="MKRTVSFFMIASMLLMAACSGKEKTKLSVPYEKYKLDNGLTVIL
                     NEDKSDPMASLVILYHVGSAREVPGKTGFAHLFEHMMFQRSENVNEDEFFKYIEGAGG
                     DLNGGTSFDQTIYFEVVPKNALELAMWLESDRMGFLRNTVTPQSFALQQNVVQNEKRQ
                     GVDNRPYGHTDAVILKNLYPQGHPYSWDVIGEMEDLANATVDDVKEFHKKFYIPNNAT
                     LVVSGDYELEEVKALIEKYFGEIPAGEELKDPEPMTVTLSETKKLYHEDGFARAPQYT
                     MVYPVAQQFTNDAYALDVLAQLLGVGKNSPLYKVLVKEKKLTSNVSAYNMSQEIAGQL
                     QISVTANPSTSLNDVEKAIFEAFGRFETEKFTERDLERIKAGIERNFYLQMSSVMYKG
                     FMMAVYNEFAGSPDFMLEELEMMKNVSMDDVWDVYNRYIKGKNYVATSFVPRGQADLV
                     AEGSVNAGIIEEDITKATQMEVVAGGEEVIEKTPSSFDRSVAPVPGPDPSITLPEIWK
                     SEASNGMKLYGIGQHELPLVVYSIVIEGGHLLDDPAKPGVARFTASMLNEGTKNRTPE
                     QLEEEIQLLGAIINIGCSDERITVSVNTLARNFEKTLALVQEMLLEPRWDEEQFSLNK
                     TRTVNNLKRNLADPNYVAGAAYDKLVLGADNILSVDLSGTVESVEAITMDDLKSFYNK
                     NISPSVANFIIVGDIDQARVEKALAGLSEGWAPKEVVIPALSFPPAPEKSAIYFVNIP
                     DAKQSVINIGNLSIPRTHPDYYKAVVANHMLGGGASAKLFMVLREEKGFTYGAYSNFM
                     GQKSYGAFQAYASVRSDATLESVELFRDIMAEYRAGVPQEIVDFTKGSLLKANALRFE
                     TIDAKLGMLNTMTFFGLPADYIRQEEDYLRGLTVEQVNETVQKYIDPMRMYYVVAGDA
                     KTQMQGLKKAGFGDPVPVN"
     gene            complement(7639..9921)
                     /locus_tag="PKK03_09600"
     CDS             complement(7639..9921)
                     /locus_tag="PKK03_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNX84696.1"
                     /translation="MKHTRIMICAIVTLLLAANAAGQVRKSALLQHDTQIDGIISRMT
                     LEEKINMLHAKHMFVSAGVERLGIADMKYADGPFGIREEMQPDGWTPLGWVNDKATFF
                     PTGSALAATWSPELAYAYGSAMAKEARLRGKDMLLGPAINIQRIPTGGRTYEYLSEDP
                     FLSSRLSVGYTKGVQDNGVAVCLKHYALNNQENNRGTVNVIIGERAMREIYLPPFRAA
                     VEEADAFGVMAAYNKVGGWWCAENDMLLNKILRDEWGFAGMVISDWGGTHSTVHSIKN
                     GLNVEMPDKKFLGQALLDSVRAGIVSEEIIDRRVREILRVRLAIRPVPEDEANKKITS
                     QPAQQMVAYEVASKSIVLLKNDGVLPLKLADKPVIAVIGANATQKMATGGLGAGVKAL
                     YEVTPLEGLKKKIGDRAEIIYVKGYEPVQYNWADRFRKRTPEELEKEARAKELTAQKL
                     SDEAVKVASGADLVLFIGGNNRAVETEGEDRKDIFLPSGQDRLITLIAEVNPNIITVL
                     TSGAPNDLNAVNPLSRALLISWFNGSEGGNALSDVLLGEISPSGRMPFTIPVKLEDSP
                     AFVLGNYPRNSRNTDVFASLVSDGDTTGQHRKEQAKDLKKDPDIAYYSEESLVGYRWF
                     DTKKIPVMYPFGHGLTYSRFEYSKLKSDKKTYGAADVITVTFKLKSTGRMLADEVVQV
                     YVHRIDPSVEWPEKELKAFERVTLAPGEAKTVKLSVPVKSLMYWNETKQTWNNDLCKI
                     NLLVGASATDIRLQKNIRLR"
     gene            10100..10184
                     /locus_tag="PKK03_09605"
     tRNA            10100..10184
                     /locus_tag="PKK03_09605"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10133..10135,aa:Leu,seq:taa)
BASE COUNT         2461 a         2506 c         2694 g         2524 t
ORIGIN      
        1 gttcccagga aagtgtcaga ccagaactca tagagcaggt attctttgcc tttttccagg
       61 ccaagttcag ccaggctgat gctctcttcc ttctcaccga gcctgccaag aacgcaccat
      121 ctctcccatg gcatattcag ttcgagcatg aacaggtcga agggtgaagt gcggctgccg
      181 tcgctctccc gttctcccga gccgctcatc tcggtggttg tgcggtcaag gaaagccgac
      241 cgtgagggat cgaggtcaaa cacctgtccc gggagtgtaa agagcaccgg cagtgacttg
      301 cgggccgggt cgatgaggcc tgtgcggtat acttccggct tgtcggtaac catgaacagt
      361 gatccggtca gcgaggtggc catgcaggag cgccaggcct cctgcggtgt cagctcaata
      421 tggtccgggt catttcgcca caccacgtta ttgaatgagt tgtactgtgc aagtgcggtg
      481 tacccgtacc cgtcattgcc tatgcggcat ccgtcggcca gcccgaccac accgggaagc
      541 actccccagc atgccagcag gaagttgttg cgcccggttt cttcgcgcac cctgcctacc
      601 acattcctga acgctttttc gcgctcagcg taaccgccgc tgaagaagct gctgtggctg
      661 ttgtacccct cgaagagcag atgacggagg gcatcgagct tgaagtagct ccacccatca
      721 tttctgaacc ggctgtagac aggcgtaatg actgaatcaa gcatctcatc atcaaggcca
      781 ttgagtatgt agccaatcca tctgccggaa gcgggggtgc cgtctgcgtt ccttacaaac
      841 cagtcagggt gggagtaagc ccaggctgag tcgtgcactg ccacgttggt ccagatgcct
      901 ggcctgaatc ctttggagct gatgtatgtt gcaaggtcgc cgagtcctga cgggaattta
      961 tcattggggt tgatccaggc ttccggtgta ccaccatact gctggtagcc gtcgtcaatc
     1021 tgaatgtatt caagtccgta cggcttcatg gtttcagcga gcacatcagt ggcttccatg
     1081 acattcttct gattgattcc ggtcttatat gcaaaccagg agcaccagcc tgcaacaggt
     1141 tctttccata cctggtaatt ccagggttca taatagtcca gtcccctgtg cttctggtag
     1201 taacggggcc tgaacctgaa ggtgacctcg ttgcccttca tgctcagggc ataggtaata
     1261 gctgtactgt cagcagtttc aggttctatt ttaagtgatg atgaggtata tgactggtca
     1321 gctgacagca gccagtcgga ctgacggtca taaacggctc tgttcagcag gctgtggctg
     1381 attccgtatg tatgcctgac tacctgggtt ccctcgatgc gtcggtcagc ctcgcaggtg
     1441 aatgactcct ttcccgcgag gatacgtcct gtgatattca ttgccgatcc gtttagtgaa
     1501 gagattgtaa tgacgcagga gactttacag tcacgctcct catcaagctc cttaacagtg
     1561 tattcagcct gaggcaggtc aatttcgagc gtcagtatgg ccgcaccgtt atagagcagg
     1621 ctgatgttgt tttcttcccg gatcagtctg gccggttcgt ttggaggagt tgtatcatct
     1681 gccggcgagg tgcacattgc cagtgcggca gtcagaatga tggcggagaa aggtagaata
     1741 tggttcatga caggttccct gagatttcca aaaataagca aaaacggact gtttcagcct
     1801 ttttcccggg ccaacgtgaa agaggtaatg tgaagaaatg ttaacggaag ccggtattaa
     1861 taaggataag ttttaaatta gctgaatatt atgcaatcag ttaacccaat ttccaaaatc
     1921 atgaagaact tgttgagagc agctttgatc ttattggttg tcactgcggt ctcctgccag
     1981 aagaaggcag ctgacgaact atcactcccg ttcgaaaaat acgagctggg aaatggtctg
     2041 gatgtaattc tgcaccagga tcattcagat cccattgtgt cggtggcaat ttactatcat
     2101 gtaggttcca accgtgaggt tccgggccgt accggttttg cacacctgtt tgagcatatg
     2161 atgttccagc agagtgaaaa tgttccggaa gatcagtatt tcaggctcat tcagacagcc
     2221 ggcgggacac tgaacggatc aaccaaccaa gaccgtacca actattatga aaccgtgccc
     2281 aaaaatgctc tggaaatggt actctggatg gagtctgacc gcatgggata cctgacaaac
     2341 accattacac agcaggcttt tgccattcag cagaacgtgg ttcagaatga aaaacgccag
     2401 aactatgaca acaggccgta cggtcattcg tcgacggtaa tggcgaaagc gttgttccct
     2461 gaaggccatc cctacagctg gacaaccatc ggtgagatga aggatctttt caacgccacg
     2521 gtggacgatg tgaaggagtt tcacgggcgt ttttacatac cgaacaatgc aaccctttcg
     2581 attgcaggcg attttgatcc cgctgaagtt aaggcattgg ttcagaagta tttcggtgag
     2641 attccggcag gagccgatgt tgagaagatg tctcccatgc ccgtaaccct tgctgagacg
     2701 aaaaaactat atcatgaaga caacttcgcc aatactgcac agtacacgat ggctttcccc
     2761 acagttgaga tgtacaatcc cgacgcctat gcactgacca ttctgagtga tatccttgcc
     2821 ggtggcaaga agtcgccgat gtacaatgtt ctcgttaaag agaagaagct tacctcaggg
     2881 gtgggtgcct ataactcggc ccagttgctt gccggcacct tccgtgtatc tgtcaacgcc
     2941 aatcccggtg tttctctcac tgatatagag aacgccatct ttgaatcctt tgcacggttt
     3001 gaagccgagg ggttcaccga gaaggacctg gagaaggtta aggcaggaca ggagacccgt
     3061 ttttataaca tgataagcag cattgacggc aaatcgaagc agcttgccga gtacaacatg
     3121 tatgccggtg accccggtta ttacaggcag gatatggagg ccctgaaggc tgtcaccatt
     3181 ggtgatgtga agaaggttta tgaaaaatat atcaagggga agaatttcgt tgccaccagt
     3241 tttgttccga aggggaaggt tgaccttgca tcggccggat cggttaatgc aggcattgag
     3301 gaggagaaca tactgactgc cacagaggta aaggtggaag ccggtgctgc cgaagagatt
     3361 aagaaaaccc cgacatcctt tgaccgttcg gttcagccgc cgcttggccc tgatgtatct
     3421 gtcacagttc cgacggtatg gaagtcatca ctggcgaacg gcatgaagct ttacggcata
     3481 cagcacacgg aagtacctct tgtacagtac gatattgtca tcgagggagg tcatatgctt
     3541 gatgatataa gtgcgcctgg cgttgccagc atgctggcaa ccatgctcaa tgaaggtacc
     3601 cggaacaaaa cccctgagga gcttgaggaa gagatagacc tccttggagc cagcatacgg
     3661 gtcggagcag gcagtgagga tattaccgtg agcgtaaata ccctcgcccg caattttgaa
     3721 aagacccttg ccattgttga ggagatgctg ctacagccca ggtgggactc agctgccttt
     3781 gaactggcca aaacccgcac cctcaacagc ctcaggagga actctataga tcccacatat
     3841 ctgggttcca gggcgctcaa caatctgatc ctgggtgaga gtaacattta cgccaccgat
     3901 gtgtccggca ctcccgtgag tgtacaggcg atgaccatgg atcagctcaa ggcattctat
     3961 gagaagaatt tttctccctc aatttcgcgc ttcctggttg tgggtgccgt tgaccagcca
     4021 agggtggaga aagcgcttgc ctcgctgaac accaactggg ctgccaaaga ggttcagatg
     4081 cctgaagtta tgcctgcaca gaaacctgaa aagtcaggca tctggttcgt gaacatacct
     4141 gatgcaaaac agtccaatat tttcatcggg gcgcctgcaa taccaagggg tcatcctgac
     4201 tactacccgg catatgttgc caactacaag ctgggaggtt ccttcaacgg ctacgtcaac
     4261 ctggtcctca gggaggagaa gggttacaca tacggagcca gaaccaacct cagtgcgatg
     4321 aagaattatg gttatttcaa tgccagctct gcagtaagat caactgcaac ccttgaatcg
     4381 gtggagattt tcagggacct gatggcgcgt taccgcgaag gtgtttcaca ggaagatgtt
     4441 gacttcacga agaatgccct tctcaagggg aatgcactca ggtttgagac ccagagggca
     4501 ctgctcggga tgatcagcac tatgagcgaa tacgggcttc ctgatgacta catcgcacag
     4561 gaggaaaact acgtcaggga acttaccgtg gagaaggtaa acgaaatggt aaacaagtat
     4621 atcgatccga tgaagatgta ctatgttgtt gccggagacg cagcaacaca gcttaaggaa
     4681 ttgaataaac tgggcttcgg agagcctgta ctggttaaat tttaaaagag atgaaaagaa
     4741 ctgtatcatt tttcatgatt gcctcgatgt tgctgatggc agcgtgcagt ggcaaggaaa
     4801 aaaccaagct gagtgttccc tacgagaaat acaagcttga caacgggctg actgtcatcc
     4861 tcaatgagga taagtctgat ccgatggcat cactggtcat actgtaccac gtcggttctg
     4921 ccagggaggt tcccggcaag accgggtttg cccacctgtt tgaacatatg atgttccagc
     4981 gttcagagaa tgtgaatgaa gatgagttct tcaaatacat tgaaggagcc ggcggcgacc
     5041 tcaatggcgg caccagcttt gaccagacaa tctatttcga ggtagtcccg aagaacgccc
     5101 tggaactggc catgtggctg gagtctgaca ggatgggttt ccttcgcaac acagtgacac
     5161 cgcagtcttt tgcgctgcag cagaatgtgg tgcagaacga gaagcgccag ggtgttgaca
     5221 acaggcccta cggccatact gatgccgtta tcctgaaaaa tctctacccc cagggacacc
     5281 cctacagctg ggatgtgatc ggtgaaatgg aagacctggc aaatgcgact gtagatgacg
     5341 tgaaggagtt tcacaagaag ttctacatac ccaataacgc aactcttgtt gtttcaggcg
     5401 attatgagct tgaagaggtc aaggcgttga ttgagaagta tttcggtgag atccccgcag
     5461 gggaggagct gaaggatccg gagccgatga cggttacact gtcagagacg aagaagcttt
     5521 atcatgagga cggcttcgcg agagcgccgc agtacacgat ggtttacccg gtggctcagc
     5581 agttcacgaa cgacgcatac gcactcgatg ttcttgccca gttgcttggc gtaggcaaga
     5641 attctccgtt gtacaaggtg cttgtaaagg agaagaagct tacctccaac gtttcggcct
     5701 acaacatgtc acaggaaatt gcaggtcagc tgcagataag tgtgactgca aacccgtcga
     5761 caagcctgaa tgatgtcgaa aaggcaattt ttgaagcatt cggaaggttc gagactgaaa
     5821 aattcaccga gagggacctt gaaaggatca aggccggcat tgaaaggaac ttctatcttc
     5881 agatgagcag cgtgatgtac aagggattca tgatggcagt gtacaacgag tttgccggct
     5941 cacccgattt catgctggag gagcttgaga tgatgaaaaa tgtctcgatg gatgatgtct
     6001 gggatgtcta caacaggtac atcaaaggca agaactatgt ggctacaagc tttgtccccc
     6061 ggggacaggc tgacctggtt gcagaaggtt cggtaaatgc aggcatcatt gaggaggaca
     6121 tcaccaaggc gacccagatg gaagttgtgg caggcggaga agaggtaatc gaaaagacgc
     6181 cgagcagctt tgaccgctca gtggcacctg ttccgggccc tgacccgtca atcactcttc
     6241 cggagatctg gaaatcagaa gcttcgaacg ggatgaagct ctacggtatc gggcagcatg
     6301 aactgcctct ggttgtctat tccatagtca ttgaaggtgg tcatttgctt gatgatcccg
     6361 caaaaccagg ggtggcccgc ttcaccgcat caatgctgaa tgagggcacc aaaaaccgga
     6421 ctcccgagca gcttgaggag gagatacagc ttcttggcgc tataatcaat attggttgca
     6481 gtgatgagag gataaccgtt tctgtcaaca ccctggcccg caactttgaa aagacgctgg
     6541 cactggttca ggaaatgctg cttgaacccc gctgggatga agagcagttc tcgctgaata
     6601 agacacgcac agtgaacaat ctcaagcgga acctggctga ccccaactat gtagcaggtg
     6661 ctgcatatga caagcttgtc ctcggggcag acaatatcct ttctgttgac ctgagcggga
     6721 ccgtcgagag cgtggaggct attaccatgg atgatcttaa gtcattctat aataagaaca
     6781 tatctccgtc tgttgccaat ttcatcattg tgggtgacat agatcaggca agagttgaga
     6841 aggctcttgc aggactgagc gaggggtggg ccccgaaaga ggtggtgata cccgctcttt
     6901 ctttcccgcc tgcaccggag aagtcagcaa tttactttgt caatatcccg gatgccaagc
     6961 agtcggtaat caatatcgga aatctctcca ttcccaggac gcaccctgac tattataagg
     7021 cagtggtggc caatcacatg cttggtggtg gcgccagtgc gaagctgttc atggtgctca
     7081 gggaggagaa ggggttcacc tacggagcct actccaattt catggggcag aagtcttacg
     7141 gtgcgttcca ggcatatgca tcggtccgct cggatgccac actggagtct gtggaactct
     7201 tccgtgacat catggctgaa tacagggccg gagtcccgca ggagattgtt gatttcacca
     7261 aaggctcatt gctcaaggcc aatgcgctca ggtttgaaac gattgacgcc aagcttggca
     7321 tgctgaatac aatgaccttc ttcggtttgc cggctgatta catcaggcag gaggaagatt
     7381 atctgagggg cctgaccgtt gagcaggtca atgagacagt gcagaagtat attgacccga
     7441 tgaggatgta ctacgtggtt gccggtgacg ccaaaaccca gatgcagggg ttgaaaaagg
     7501 ctggcttcgg tgacccggtg ccagtgaatt aatctgattg catgttatag ttacagagtt
     7561 cccggtttgc attattgcaa gccagggact ctgtaatgtt ttttgccggt atttgcgagc
     7621 agttcaggtg tgtggtgatc acctgagcct gatatttttc tgaagtctta tatctgtggc
     7681 cgatgccccc acaagcaggt ttatcttaca cagatcatta ttccaggtct gttttgtttc
     7741 gttccagtac atcaggctct ttaccgggac tgaaagtttt actgttttgg cctcgcccgg
     7801 ggcaagggtt accctttcaa atgccttgag ttccttctca ggccactcca ctgaaggatc
     7861 tatgcggtgc acgtagacct gcacaacttc atccgccagc attctgccgg tgctcttcag
     7921 tttgaatgtt actgtaatta catcggctgc tccatacgtt tttttatcgc tcttcagttt
     7981 tgagtattcg aaacgggaat aggtaagacc gtgaccaaag gggtacatta cgggaatttt
     8041 cttcgtatca aaccagcggt agcccacgag tgattcttca gaataatacg ctatgtcagg
     8101 gtccttcttc aggtcctttg cctgttcctt ccggtgctgt ccggttgtgt cgccgtctga
     8161 aaccaggctg gcaaaaacat ctgtgttccg gctgttccgg ggatagttgc cgaggacgaa
     8221 tgccggggaa tcttcaagct ttaccggtat tgtgaacggc atccgccccg aaggagaaat
     8281 ctctccaagg agcacatcgg ataatgcatt tcccccttcg gatccgttga accaggagat
     8341 cagtaatgcc ctggatagag ggttaactgc attcagatca ttcggggcgc cactggtcag
     8401 cactgtgatg atattcgggt tcacttcagc aatgagggtg atcagcctgt cctgtcctga
     8461 gggaaggaat atatccttcc tgtcttctcc ttcggtctcc acagcgcggt tattaccgcc
     8521 aataaacagg accaggtctg ctccggaggc aaccttaaca gcctcatccg agagtttttg
     8581 tgctgtcagt tcttttgccc tggcttcctt ctcaagctct tccggtgtcc gtttccggaa
     8641 cctgtcggcc cagttgtact gaaccggttc ataccctttt acatagatga tctcagccct
     8701 gtctccaatc ttcttcttca gtccctccag cggggtcact tcataaagag ccttgacacc
     8761 tgccccaaga ccacctgttg ccatcttctg tgtggcgttt gcaccgataa ctgctatcac
     8821 cggtttgtca gcgagcttaa gcggaagcac accatcattt ttcagcagga cgattgattt
     8881 gctggccact tcataggcaa ccatctgctg tgccggctgg gaagtgattt tcttgtttgc
     8941 ttcgtcctca ggaacaggcc tgatagccag tcttacacgc aggatctcac ggacgcgcct
     9001 gtcaattatc tcttcggata caatacccgc ccggactgaa tcgagcaagg cctggcccag
     9061 aaatttttta tcaggcattt cgacattaag accgtttttg attgaatgca ctgtgctgtg
     9121 agttccgccc cagtcggaaa ttaccattcc ggcaaagccc cattcgtcgc gcagaatctt
     9181 attgaggagc atgtcatttt cagcgcacca ccagccgccg accttgttgt atgcagccat
     9241 caccccgaat gcatcagcct cctcaacagc ggctctgaac ggagggaggt agatctcgcg
     9301 cattgcccgt tctcctatta tcacgtttac cgttccgcga ttgttttcct ggttgttcag
     9361 ggcgtagtgc ttcaggcaga cggcgactcc gttgtcctgg acgcctttag tgtacccgac
     9421 tgacagccgg gaactcagga aggggtcttc gctgaggtat tcatatgttc ttccgccggt
     9481 aggtattctc tggatattga ttgcaggtcc gagcagcata tcctttccac gcagacgtgc
     9541 ctccttcgcc attgctgatc cgtaggcgta cgccagttcc gggctccagg tcgcggccag
     9601 ggctgatcct gtcgggaaga aggtggcttt gtcgttgacc cagcctagag gcgtccagcc
     9661 gtcaggctgc atctcctccc ttataccgaa cggaccgtca gcatatttca tatctgcaat
     9721 tccgagccgt tccaccccag ctgacacgaa catgtgcttt gcatgcagca tgtttatttt
     9781 ttcttcgagg gtcatccggc tgattatgcc atctatttgt gtgtcatgtt gaagcagggc
     9841 tgatttgcgg acctgtccgg cggcgttggc tgccagcagt aaagtgacga ttgcacagat
     9901 catgattctg gtatgtttca tgaggttttt gggtgtttga tcattcatta agcccgggaa
     9961 ttttaaaagg cagccgtaaa tataatttac atatcctata tttgcacaac catatgaaag
    10021 atcattgtcc ggcaggtttc tggacaataa gacctgagac cttcagacct cagaccttgc
    10081 gactttcaga ccttccattg cccggatggt ggaatggtag acacgctgga cttaaaatcc
    10141 agtgggcagt aatgcctgtg cgggttcaag tcccgctccg ggtac
//
LOCUS       DAOPXU010000099        10130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_7762_length_10130_cov_14.928734, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000099 DAOPXU010000000
VERSION     DAOPXU010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10130
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7762_length_10130_cov_14.928734"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1526
                     /locus_tag="PKK03_09610"
     CDS             <1..1526
                     /locus_tag="PKK03_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HNX84697.1"
                     /translation="NYRDISGDPAARCEKILADVRGKNFKTLKNTHLDDFTTLMSRVS
                     LKIGDPVKNEIPTDARVAALKKGESDPDLLAKIFQFGRYMIVSGSRDGSEPLNLQARW
                     NQDLLPNWGSKYTININTQMNYWPAEVTNLSECTGPLFNLIKDLSENGAQTAKLYYNA
                     GGWVAHHNTDLWRGTAPVDAARYGMWPVGGAWLCQHIWEHYLYTGDKEFLREYYPVMK
                     GSAQFLMDIMTVNPAYDYLVIPFSMSPEQGFFVSEGAEEAFLAPATTMNTGLINDLFP
                     NCIEAAGLLDTDADFSKSLGEALGRIPPYMIGRDSLLQVWIEDWKRGNEGHDMSANFG
                     FYPGNSITLRDNPDLAAAIRKWLEPRRASTSWPTAWDISFWARLEDGARADTVIRKFF
                     MARSSWGGIGNNLHNTAFNQSDANFGYTAGVAECLLQSHAGEISILPALPASWKDGSV
                     TGLKSRGGFEVDIDWKDGKLVKAEIRSLLGNPCTVRANGTTKEYSPAIGEKVVINGI"
     gene            complement(1596..3302)
                     /locus_tag="PKK03_09615"
     CDS             complement(1596..3302)
                     /locus_tag="PKK03_09615"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNX84698.1"
                     /translation="MKKSLTILALVLASCSGETTEKEATYCNPLNINYRFQYTDSVSY
                     REAADPTMIRFKDKYILLVSHSGGYWFSDDLHGWTYLPVSTLPIEDYAPDAIAISDTV
                     WFAASSAERKPFYYTTDPFSDNWKPLNDTLPFAVWDPHFFADSDGQRYLYWGCSNQLP
                     IYGVKLNSKMQAVSEPQVLIGHNADIYGWEVPGEKNELTTGGWNEGAWMTKYNNRYYL
                     QYAAPGTEYRTYADGVYTSDSPLGPFAYETYSPFSYKPGGFAGGAGHGSTFPDEYGNY
                     WHIATMSISIRHMFERRLGLFPAAFDKDGVLRTFTAFGDYPAIMPDRKVDFEKENTFT
                     GWMLLSYNKKAEASSAMDEFPVSNAFDEDIRTWWSAVTGNSGEWLSVELDDASTVYAV
                     QVNFADNGSKLKPGSKEIFYRYRILASKDGKSWDVLADKSNNTADACHDYIQLEKPVQ
                     TRFIKIENVSVPDGTFSLYDLRIFGTREGNTPAEVNEFTVARNEADTRKAIVEWPADD
                     RATGYVVNYGTSPQKLYTSIMVYDSGSVMLTGLNRDVKYYFTVDAFNESGITRGMTVV
                     EK"
     gene            3554..5083
                     /locus_tag="PKK03_09620"
     CDS             3554..5083
                     /locus_tag="PKK03_09620"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HNX84699.1"
                     /translation="MKTIKSSFMKKLPFLLGCMILSCLFPDTLKSQDKAVIKIDFDRK
                     IGTVDPNIYGAFVEPIMKVVYGNIYDPASPFADENGFRKDFIQLVRELKIPVVRWPGG
                     NYVSGYNWEDGIGPKDKRPARLDLAWHQVESNHMGTDEYVQLCKLIGAENFICINGGT
                     GTLDQARHWVEYCNLEKGTYYSDLRRKYGNEEPFKVKYWALGNEIDGPWQMGQKSAED
                     YVKFALEAGKLMQLVDKDIKLIASGASNYRPDNGWIDWNDYVLTHMTGTIDYLSVHRY
                     ATEALGDDRSFSGMMSLGLDLDQKIEAVKALILKAMTKTGSTRPVYISFDEWASGFGN
                     SITVSLMVAQHLNSFIRHADIVRMANMTMLSNLVGYSPEGVYKNATFRAFWLYSNNCF
                     GTSLDVSTSCEAYSNNIFSEIPYLDVTAILNSEAGNVVVNVVNRHETKAIETDIILQT
                     GDFAGNAKVNEVNGKIADPGDPLAEEAVNSTSSDIRFKGNTIKYSFPAHSFTQIMIPF
                     K"
     gene            5357..8575
                     /locus_tag="PKK03_09625"
     CDS             5357..8575
                     /locus_tag="PKK03_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017141023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX84700.1"
                     /translation="MEQKLTKPILLLRTGMQKHFLIAKLVLLTFALSILGGNAYSLGA
                     DDLNLGSDNILQQIRVAGVVKDANGQPMPGVAVKVTGTTLGALTDANGRYTLDVPGAS
                     ATLEFSFIGYAVVTAPVNGRSVIDVTLQETTEALDEIVVVGYGTVRKSDVTGAVASVR
                     ADAIKNVSTTDAAAALQGKAPGVQVLSNSGAPGQAATIRVRGYSSNSGNIGPLLIVDG
                     LKVDNIQYLDPSMIESIEILKDAASAAIYGAQAGNGVVLITTKSGTKGASSITYDFKY
                     TGQSLANKPEIFRAKDWINYKRMSGYDMDAMLTANHYDNTDTDWFDAVFAPSWSPQHT
                     LTFQGGNNQGQFFTSVNYVNNDGMVKGDRDVYKRLTAQINADYNVKEWLQVGTNNSIE
                     NWNTKSVSQMSQYGSVMNSVMTLDPLTPVYYSSPDQFSSSMKQAYDAGRPILKDPTND
                     LYYATSKYITDDSGNPLLQRDRVDRKNGGINLRGVVYGNLKPLKGLVITSRFGYRVGQ
                     NTVSSYNTPYFATPQASTYDYNISADANTNYYYQWENFANYNLVLGKHNATAMAGMSY
                     TENNSFNVSASASGPNILSGYEPNFRYLNYVISNVVDGTEKTVKSFSNMPSQSTQISY
                     FGRLIYNFANKYSIQGNFRADAFDSSKLSAQNRWGYFPSFSAGWIISNEEFFAGVADR
                     IPVTFLKLRGSWGLNGNINVLNNYPYSTSISYNSSWYQYMVDDGAPEYGSAPSGLANP
                     NLKWETSEQLDFGLETRFLSDRLSFSAGYYNKKTNDLLVTISPVPEIGVSSTVVNAGS
                     VLNSGLEFEAGWKDKAGDFTYAINANLFTLHNEVTYLDPAISRLTDNKGGVSGTNNEV
                     ESAFEVGYPIWYFRGYKYNGVDPATGDAIIEDVNGDETISDADMTYLGKAIPDYTYGI
                     TVNLGYKGFDLNVFGNGVAGNDIFTVFYRADTPMRNSLRYYYDNAWTPDNTGASMPDP
                     SKVATSWKFWSSSAAMFNGAYFKIKQIQLGYTLPASITNRILVNRLRCYVSLDDYLTF
                     TKYPGADPETATASNNSASAAGYDNGTYPQARKVIFGVNITF"
     gene            8593..>10130
                     /locus_tag="PKK03_09630"
     CDS             8593..>10130
                     /locus_tag="PKK03_09630"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX84701.1"
                     /translation="MKKNKYILVLLLVMLFAACSEDRLEIPQKGIISIDSFYKTDADA
                     ESALVAMYAHFATQIASWEGAYIYVPLNILLNYPADNILAAGEFYGDNDMVAAINEFR
                     FDTQSPVIRLAYNHFYYVIYRANLIIDNFQYGDSPVKDRCISEARVMRAWCHMMAAMA
                     WGNPPLVDHVLQGSDKPTNYEGGHEALLQWCADECADAVQYLDERQSKADKDGAVKIT
                     KGFAWTVQGKALMYKGDYPNAKIALKKVISSDKYDLVPGEEWADIFHIGGDGSEEKIF
                     ESNIAVNASIGDWGGKIQRSTWMEMNIWGWRTSRMIAQPKYMAAQGWGGLAIEDEFAD
                     EFYANDGDSYRRKGTMINYQEFLTELEWPSDNGNINGMTVAQKLADPLRGINNVQGLY
                     GQCSFLQKKHIATPADVLTTSSYRYNNYIVARYADVLLLYAEACAQTSDNDGLQYLQK
                     VQQRAGSAHVSTALTLNDVKKERNYELWCEGARWIDMKRWGELDKAKNAGKHIPSLKD
                     AFFV"
BASE COUNT         2799 a         2389 c         2418 g         2524 t
ORIGIN      
        1 ttaattacag ggatatcagc ggagatccgg cagccaggtg tgaaaagatc cttgcagatg
       61 tcaggggaaa gaacttcaaa acacttaaaa acacccatct agatgatttc accaccctga
      121 tgagccgggt gagtctcaag atcggtgacc cggtgaaaaa tgagattccg accgatgcaa
      181 gggttgctgc tctgaagaaa ggagaatctg atcctgacct tctggcaaag atatttcagt
      241 ttggccggta tatgattgtt tccggcagca gagacggcag tgaaccatta aacctgcagg
      301 cccgctggaa ccaggacctg ctgcccaact ggggcagcaa atacacgatc aacataaata
      361 cccagatgaa ttactggccg gctgaggtga caaacctgtc ggaatgcaca ggtcccctgt
      421 tcaatctgat aaaggacctg tctgaaaacg gggctcagac tgcaaaactg tactacaatg
      481 caggtggctg ggtagctcat cataatactg atctctggag agggaccgcg cctgttgacg
      541 cagcccgtta cggaatgtgg ccggttgggg gagcatggtt atgtcagcat atatgggaac
      601 actacctcta caccggggac aaagaattcc tcagggaata ttacccggtg atgaagggat
      661 ctgcacagtt cctgatggac ataatgaccg taaatccggc gtatgattac ctcgtcattc
      721 cgttttcaat gtcacccgag cagggattct tcgtaagtga aggtgcggaa gaggcattcc
      781 ttgccccggc aacaacaatg aatacaggac ttattaacga tctctttcct aattgtatcg
      841 aagccgccgg gttacttgac actgatgcgg acttcagcaa aagtctcggg gaagcccttg
      901 gcaggatccc gccgtacatg atcggcaggg acagccttct ccaggtgtgg atagaagact
      961 ggaaaagggg aaatgaaggt cacgacatgt cagccaattt cggattctat cccggcaact
     1021 ccataaccct tagggataat cccgacctgg ccgccgcaat cagaaaatgg cttgaaccgc
     1081 gccgggcctc aacatcatgg ccaactgcat gggacatcag tttctgggcc aggctggagg
     1141 acggcgccag ggctgatacc gtcatcagga aattcttcat ggcacggtca tcatggggag
     1201 ggatcggaaa caaccttcac aacacagcct tcaaccagtc agatgcaaac ttcggataca
     1261 cggccggagt ggcagagtgc cttttacaga gtcatgcggg agaaatatcg atactgccgg
     1321 ccctgcctgc aagctggaag gacggatcgg tgaccggact gaagtccagg ggtggtttcg
     1381 aggttgatat agactggaag gacggaaaac tcgtgaaggc tgagatcagg tccctgctcg
     1441 gcaatccatg caccgtcagg gctaacggca caacaaagga atacagccct gccataggtg
     1501 agaaagttgt aataaacggc atttaaccat tgaaaagagg gaggccggtt tcaccgtgac
     1561 ctccctcttt aatacgccat caaaactgcc tgcggctatt tttccacaac tgtcataccc
     1621 ctggtgattc ccgattcgtt gaaggcatca acagtgaaat aatatttcac atcccggttc
     1681 agacctgtca gcataaccga accggaatca tataccatga tggaggtata tagcttttgg
     1741 ggcgaagtgc catagtttac tacatagcct gttgcccggt catctgcagg ccattccacg
     1801 attgcttttc tcgtgtcagc ttcgttgcgc gccacggtga attcattcac ctctgcggga
     1861 gtatttccct ccctggttcc gaagatccgc agatcataga gagagaaggt gccatcagga
     1921 acactgacat tttcaatctt tatgaacctg gtctgaacgg gtttctcaag ctgtatgtaa
     1981 tcatggcatg catcagcagt gttattgctc ttgtctgcaa gtacatccca tgattttcca
     2041 tcctttgatg ccagaatcct gtaacggtaa aagatctctt tgctgccggg cttgagtttt
     2101 gatccgttgt ctgcaaagtt gacctgaacg gcatacacag tggatgcgtc atcaagctcc
     2161 acgctgagcc attccccgct gttgccggta acagctgacc accatgttct tatatcctca
     2221 tcgaaggcat tgctgacagg gaactcatcc attgccgatg aagcttcagc cttcttatta
     2281 tatgacagaa gcatccatcc agtaaaagtg ttctcttttt caaaatcaac cttcctgtca
     2341 ggcatgattg ccgggtagtc accgaaagca gtaaatgtcc tcagtacacc gtctttgtcg
     2401 aaagctgccg ggaacaaccc aaggcgcctc tcgaacatat gacggattga aattgacata
     2461 gttgctatat gccagtagtt gccgtattca tccgggaatg tcgaaccgtg ccctgccccg
     2521 ccggcaaatc ctccaggctt gtaagagaat gggctgtagg tctcatacgc gaacggtcca
     2581 aggggcgaat ccgaagtata aacaccgtca gcataagtcc tgtattcagt tcccggtgca
     2641 gcatactgaa ggtagtaccg gttattgtat tttgtcatcc atgctccttc attccatccg
     2701 ccggtcgtca gctcattctt ctctccgggt acctcccacc cgtagatgtc ggcattatgt
     2761 ccgatgagca cctgcggttc agagacagcc tgcatttttg aattgagctt tactccatat
     2821 atgggaagtt gatttgagca accccagtaa agatatctct gcccgtcact gtcagcgaag
     2881 aaatgaggat cccaaacggc aaacggcaac gtgtcattca ggggtttcca gttatcactg
     2941 aaagggtctg tggtataata aaatggcttc ctttctgcag atgatgcggc aaaccagact
     3001 gtatcactga ttgcaattgc atcaggtgcg tagtcctcaa tgggaagggt actgacaggc
     3061 aggtaggtcc acccgtgaag atcatcagaa aaccagtacc ctccgctgtg ggaaactaag
     3121 agtatgtatt tatccttgaa ccgtatcatt gtgggatcgg ctgcctcacg gtatgataca
     3181 ctgtcagtgt actgaaaccg gtagttgata ttcagcggat tgcagtaggt tgcctccttt
     3241 tctgttgttt ctcccgaaca ggatgccaga accagggcga ggattgttaa cgactttttc
     3301 atgattcgat gggtttagaa gcctgtattc tgtcagactt cgcatctttt ccagatgata
     3361 tcgaaagata gaaaaacttg tcatgaaagc acatcgtgca gccataaaaa ccagccccgc
     3421 gtgaagtttt tgctgatgat atgttcagca tgaaggattt ttgaaaccaa tgacgtaaaa
     3481 gtaaaagcaa tattctgtgg gtgaccacat ccggagccgg cggatttcat tatatttcaa
     3541 attgattaac gatatgaaaa ccataaaatc atcattcatg aaaaagctgc cttttcttct
     3601 gggttgcatg attctttcct gcctgtttcc cgacactctg aagtctcagg ataaagccgt
     3661 tataaaaatc gattttgatc gcaaaatcgg cacggttgat ccaaacatct acggagcctt
     3721 cgttgagcct ataatgaaag tggtgtatgg taacatctat gaccctgctt ctcccttcgc
     3781 tgatgaaaat ggtttcagga aagacttcat tcagcttgtc cgtgagctga aaattccggt
     3841 tgtcagatgg cctggcggaa actatgtttc agggtacaac tgggaggatg gcatcggccc
     3901 gaaggacaag cgtccggcaa gactcgatct tgcctggcac caggttgaaa gcaaccatat
     3961 gggaactgat gaatatgtgc agctctgcaa gctcatcgga gcggagaatt tcatatgcat
     4021 aaacggagga acgggcaccc ttgaccaggc gagacactgg gtggaatatt gcaaccttga
     4081 aaaaggcact tattattctg acctgagaag aaagtacggt aacgaggaac cttttaaagt
     4141 aaaatactgg gcactgggaa atgagattga cgggccatgg cagatgggac agaaaagtgc
     4201 cgaagattat gtgaagttcg cacttgaggc cggaaagctg atgcagttgg ttgacaagga
     4261 cataaagctg attgccagcg gcgcttcaaa ttacaggccg gataatggct ggatagactg
     4321 gaatgactat gtgttgactc atatgacagg caccattgat tacctctcag tccaccgtta
     4381 tgcaaccgaa gcactcggtg atgacagaag cttctcaggg atgatgagtc tcggccttga
     4441 ccttgaccag aagatagagg cagtgaaagc tctcatcctc aaagcgatga caaagacagg
     4501 ttcgacacgg ccggtttata tttccttcga tgagtgggct tccgggttcg ggaacagcat
     4561 caccgtctca ctgatggtgg cacaacacct gaactcattt atccggcatg ccgacatagt
     4621 caggatggct aacatgacaa tgctctcaaa ccttgtggga tattcacctg agggggttta
     4681 taagaacgca accttcaggg cattctggct ttacagcaat aactgcttcg gcacttcgct
     4741 cgatgtcagt acttcatgtg aggcatacag caacaatatc ttcagtgaaa ttccgtacct
     4801 tgatgttact gcaatcctta acagcgaagc cggaaacgtt gtggtcaatg tggtcaacag
     4861 gcatgagaca aaggccattg aaacggatat aatcctgcag acaggagact ttgccggcaa
     4921 tgcaaaagtg aacgaggtaa acgggaaaat agccgacccc ggagatcccc tggcggaaga
     4981 ggctgtaaac agcacatcat ccgacatcag attcaagggg aataccatta aatattcatt
     5041 cccggctcat tcattcacac agatcatgat cccgttcaaa tgaacgataa ttcactttaa
     5101 ggtgatttga atgattttta aatctctttg actaaaaaac aaaagccttt atggcaagtt
     5161 tttcattgct ttgaaagtgt tagagctaca cttactggta gactcccttt cagtaattgc
     5221 aaaatttaaa cctgattgcc tatgaaacac ccttcgaaac ctgacctcaa cctgtggatt
     5281 tggaatgtcc atgaaattta atttacagta cttacttcaa taaatggaaa ctcccaacta
     5341 actaaacaac caagttatgg aacaaaaact caccaaacct attcttctat tgagaacagg
     5401 aatgcagaaa cactttctaa ttgcaaaatt agttcttctc acctttgccc tgagtattct
     5461 tggaggcaac gcatatagcc ttggggctga tgatctgaat ctcggttcag ataacatact
     5521 tcagcaaatc agggtcgccg gggttgtaaa agatgctaac ggacagccca tgcctggtgt
     5581 tgccgtaaaa gttacaggaa caactttagg agcattgaca gatgccaacg gaaggtatac
     5641 tcttgacgta ccgggagcca gtgcgacact ggaattctct tttatcggtt atgcagtggt
     5701 gacagcgcct gtaaatggca ggtcagttat tgatgtcacg cttcaagaga ccacggaggc
     5761 actggatgaa attgttgttg tgggctatgg caccgtgaga aagagcgatg ttaccggcgc
     5821 tgtagcatca gtccgggcag acgccatcaa aaatgtatct accactgacg cagccgcagc
     5881 tcttcagggc aaggctcccg gagtccaggt tctttccaac tcaggtgctc ccggtcaggc
     5941 tgcaaccatc cgtgtacgcg gttactcttc aaactcgggt aatatcggtc ctctccttat
     6001 tgtggacggc ctgaaggtcg ataatatcca gtacctggat ccttcgatga ttgaatccat
     6061 cgagatattg aaggatgctg cttcagctgc catttatggt gcccaggcag gtaacggagt
     6121 cgttctgatc acgacaaaat ccggtaccaa aggcgcttcc agcattactt atgatttcaa
     6181 atacaccggc cagagccttg caaacaaacc ggaaatcttc agagcaaagg attggatcaa
     6241 ttataagaga atgtcaggtt atgatatgga cgctatgtta acagcaaatc attatgataa
     6301 tactgacact gactggttcg atgcagtgtt cgctccttcc tggagcccgc agcatactct
     6361 cactttccag ggcggaaaca accagggaca attcttcacc tctgtcaatt atgtgaataa
     6421 tgacggtatg gtaaagggtg acagggatgt ttacaaacgt ctcactgcac agatcaatgc
     6481 tgactataac gtaaaagaat ggttacaggt aggtaccaac aactctattg agaactggaa
     6541 taccaaatcg gtttcccaga tgtcacagta tggttcagtc atgaactcgg tgatgacact
     6601 tgaccctctg actccggtat actattcttc gccggatcag ttctcatcat cgatgaaaca
     6661 ggcatatgat gcaggcaggc caatactcaa agatcctaca aatgatctct actatgcaac
     6721 atcaaagtat ataacggacg atagcggtaa ccccctgctt cagagagacc gtgttgaccg
     6781 gaagaacgga gggatcaatc ttcgtggtgt tgtctatgga aacctgaagc ctttgaaagg
     6841 gcttgtaata acctcccgtt tcggttacag ggtgggacag aacactgtca gcagctacaa
     6901 cactccgtat tttgccacac cacaggcatc gacatatgat tacaacatct cagctgacgc
     6961 aaataccaat tactattatc agtgggaaaa ctttgccaac tataatcttg tcctcggcaa
     7021 acataacgca acagcgatgg ctggtatgtc ctatactgaa aacaattcat ttaacgtcag
     7081 tgccagtgcc agcggaccca atattctgtc agggtacgaa ccgaacttcc gctatctcaa
     7141 ttatgtaata agcaatgtgg tagacggaac tgaaaagact gtcaagagtt ttagcaatat
     7201 gcccagccag tctactcaga tatcatactt tggacgactc atctacaact ttgccaacaa
     7261 atactcaatt cagggtaact tccgtgctga tgcgttcgac tcatccaaat tatcagctca
     7321 aaaccgctgg ggttacttcc cgtcattctc agccggctgg atcatcagca acgaagaatt
     7381 ctttgcgggt gtcgctgacc gtattccggt cactttcctg aaactccgcg gttcatgggg
     7441 gctgaatggt aatattaatg tgctgaacaa ttatccgtac agcacaagca tctcttacaa
     7501 cagctcatgg tatcagtata tggtagatga cggtgctccc gagtacggtt ccgcaccctc
     7561 aggccttgcc aacccgaatc tgaaatggga gacctcggaa cagcttgact ttggtcttga
     7621 aacgagattc ctgagtgaca ggctgtcttt ttcagcaggc tactataaca agaagaccaa
     7681 tgaccttctc gtcacaatca gccctgttcc tgaaataggt gtttccagca ctgtagtcaa
     7741 tgcaggatct gttctcaaca gcggtctgga atttgaagca ggatggaaag acaaggcagg
     7801 tgatttcacc tacgctatca atgcaaacct gtttacactg cacaacgagg ttacctatct
     7861 cgatcccgcc atatcgcgcc tgactgacaa caaaggtgga gtaagtggca ccaacaacga
     7921 ggtagagtca gcattcgagg ttggttaccc gatctggtat ttcagaggtt ataaatataa
     7981 tggtgttgac cctgcaaccg gtgacgctat tatcgaggat gtcaacggag acgaaacgat
     8041 aagtgatgct gatatgacct atctcggtaa agcaattcca gactacacat acggtatcac
     8101 cgttaacctc ggttataagg gattcgacct gaacgtattt ggcaacggcg tagctggcaa
     8161 tgatatcttc actgtgttct atcgtgctga tacccctatg cgtaactctc tcaggtatta
     8221 ttatgataac gcctggactc ctgataacac cggcgcctca atgcctgatc cttcaaaggt
     8281 ggcaaccagc tggaagttct ggagctcttc agctgccatg ttcaacgggg catacttcaa
     8341 gatcaagcag atccagctgg gttacaccct tcctgcttcg attaccaaca ggatacttgt
     8401 caacaggctg agatgttatg tgtcgcttga tgactacctt acctttacca agtaccccgg
     8461 agctgaccct gaaacagcta cagcctccaa caacagtgct tcggccgctg gttatgataa
     8521 cggcacttat cctcaggcca ggaaggttat tttcggagta aatattacat tctaacttaa
     8581 ttcttaagca agatgaaaaa gaataaatat atactggtct tactactcgt gatgttattc
     8641 gctgcatgca gcgaggacag gctcgaaatt ccacagaagg gtatcatttc catagattca
     8701 ttttacaaga ctgacgcaga tgcggaatcg gcgcttgtag ccatgtacgc gcattttgcc
     8761 acacaaatag caagctggga aggtgcatat atctacgttc ctctaaatat cctcctcaac
     8821 taccccgctg acaacatcct tgcagccgga gaattctacg gtgacaatga catggtagcc
     8881 gccatcaacg agttccgttt tgatacacaa agcccggtta tcaggctcgc gtacaaccat
     8941 ttctactacg ttatctatcg ggctaacctg atcattgaca acttccagta cggcgacagc
     9001 ccggtaaaag accgctgcat ttctgaggca agggtgatgc gcgcatggtg tcatatgatg
     9061 gccgcaatgg cctggggtaa ccctcctctt gttgaccacg tgctccaggg ctcagacaag
     9121 ccaacgaact atgaaggcgg acatgaagct cttcttcagt ggtgtgcaga tgagtgtgca
     9181 gatgctgtac agtaccttga cgaacgtcag agcaaggcag ataaagatgg cgctgttaaa
     9241 attaccaagg gctttgcctg gacagttcag ggaaaggctc tcatgtacaa aggcgactat
     9301 cccaatgcaa aaatagccct taagaaagta atttcttcag ataaatatga ccttgttccc
     9361 ggtgaagagt gggctgatat cttccatatt ggcggtgacg gttccgaaga gaaaatcttc
     9421 gagtcgaata ttgctgtgaa cgcctccata ggtgactggg gtggaaaaat ccagcgctct
     9481 acctggatgg aaatgaatat ctggggttgg agaacttccc gaatgatagc ccagccgaaa
     9541 tacatggctg ctcagggatg gggcggtctg gccattgaag atgagttcgc cgatgagttt
     9601 tatgccaatg acggagattc atatcgccgc aaaggcacta tgataaatta tcaggagttc
     9661 ctgaccgaac ttgaatggcc aagtgataac gggaatataa atggcatgac cgtagcacag
     9721 aaactcgcag accccctacg tggtatcaat aatgtccagg gcctttacgg acagtgcagt
     9781 tttcttcaga aaaagcatat cgcaactcct gcagatgtgc tgacaacctc ttcgtaccgg
     9841 tacaacaact acattgttgc aaggtatgct gacgtccttc tcctttatgc ggaggcctgc
     9901 gcccagacaa gcgacaacga tggtctgcag tatctccaga aggttcagca gcgtgcagga
     9961 tcagctcatg tcagtaccgc tttgactctg aatgatgtca agaaggaaag aaactatgag
    10021 ttgtggtgtg aaggcgcacg ttggatagat atgaagcgct ggggtgaact tgacaaggca
    10081 aagaatgcag gaaagcatat tccttcactc aaagatgcct tctttgttga
//
LOCUS       DAOPXU010000100        10125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_7769_length_10125_cov_13.711321, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000100 DAOPXU010000000
VERSION     DAOPXU010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10125
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7769_length_10125_cov_13.711321"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..306
                     /locus_tag="PKK03_09635"
     CDS             106..306
                     /locus_tag="PKK03_09635"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HNX84702.1"
                     /translation="MAVLKVIEILASSPVSWEDATASAVKMAAKTVKEIRSVYVQDQS
                     AVVVKNKITEYRVNVKITFEVI"
     gene            321..782
                     /gene="lysM"
                     /locus_tag="PKK03_09640"
     CDS             321..782
                     /gene="lysM"
                     /locus_tag="PKK03_09640"
                     /inference="COORDINATES: protein motif:HMM:NF008399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HNX84703.1"
                     /translation="MGLISFIKNAGAKIFKSEEKREEEKANMITEHIKKFGFDVSRVS
                     VAVDDEKVVLTGSVDTLSNKNKIIVTAGNVEGISVVEDHLLVTTPEPVAPPEPEKQFY
                     TVKKGDYLSKIAKEVYGDAKKYPIIFEANKPMLKDPDLIYPGQVLVIPPLT"
     gene            881..1363
                     /locus_tag="PKK03_09645"
     CDS             881..1363
                     /locus_tag="PKK03_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84704.1"
                     /translation="MRKIRIISPFISLFLITLILSGSFGFTLIHHTCIHCGTNETIAS
                     LAGDPVGDNCCGNHGNSCCGDQKATSCSHGDCDVQHRHSTGDAVLSDDCCTHETERVV
                     TDELVRTEAQNEILPYFLAATIIAVMEEHQTNNYNHFSGEQPFHCGRDLTTMLCRIRS
                     "
     gene            1498..3756
                     /locus_tag="PKK03_09650"
     CDS             1498..3756
                     /locus_tag="PKK03_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX84705.1"
                     /translation="MKHLITLITILLIPAFAVAQGRLEGKVVVAGSDGAPDGEGLPGA
                     NVVWAGTTVGASANAAGYFTIRKVKDHNLLVASFVGYTSDTVMIDPGTDYIEIILKEN
                     SMIDEITVIGRATGTHIDREATIATVNITAAELCKAACCNLSESFVTNASVDVNYADA
                     ATGAKQIQLLGLAGSYVQILTENIPAMYGLGAAYGLSYIPGPWMESIQVSKGTSSVRN
                     GNESITGQINVEYKKPATSEIVFVNGFVSDAGKIETNANASLRLNQYWSTMLLGHGET
                     QSGASDHNKDGFRDEPDVRQYSFMNRWDYLSNNLTFRAGIKYMNEERIGGQFDYSRND
                     PNTWDSGYGINMATERAEAFTKLGGMFGPSDNMSLGWIQSFTFHEMNSWLGYNKYDAT
                     QKSYYTNLLYQYFPAEKHTVDAGVSYRFDHYDELQGIYGPLTGQVLTNINAETVESVP
                     GIFMQYTYTDSAKVTLLAGVRYDFYRNHGAQFTPRLHLRYEITPHMTLRASAGKGYRT
                     VHVLAENMYFLSSSRQMVIADNLDIEEAWNAGINLTGYIHIGDRELRLTGEAYRTSFI
                     KQIVTDLDSDVDRVSFYNLDGKSYSNVIQLEAQMQPVTGLDITAAWRWNDVRQTIGGD
                     LREKPLASRYKGLLTASYTTRMRKWQADYTLQYNGPGRLPSTMANPEPYRRHDQFGAY
                     PVMNAQVTRYFKKWSVYIGAENITGSRQHDAIIAADDPFGPYFDGSMIYGPVHGRKLY
                     AGFRFALDRPQN"
     gene            3783..4124
                     /locus_tag="PKK03_09655"
     CDS             3783..4124
                     /locus_tag="PKK03_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HNX84706.1"
                     /translation="MKSNLIITAVLLFLFSSLTLSAQEKNKKDVSTVRFATSIDCQNC
                     VNTIMKNVPFEKGVKDVKCDLATKEVTVQYQKDKISPETLKRSIEKLGFTAKEVTEKK
                     KEEVTPQGSRQ"
     gene            complement(4166..4897)
                     /locus_tag="PKK03_09660"
     CDS             complement(4166..4897)
                     /locus_tag="PKK03_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNX84707.1"
                     /translation="MEGWLSRTEMLLGPEALQRLKDSHVLVAGLGGVGSWAAEMICRA
                     GAGAMTIIDGDVVTAANRNRQLPALTSTTGRKKAEVMGERLLDINPDLRLRIIAEFIR
                     DQRMIDILEEAKYNYVVDAIDTLSPKVFLVYHSLRLNLRVVSSMGAGGKYDPTAIRVA
                     DISETNFCNLARMLRKKLHKLGVHEGFTAVYSPEAVDKNLIVRGSDESNKASSVGTIS
                     YMPAAFGIACASVVIRELAGITGAV"
     gene            complement(4897..5568)
                     /locus_tag="PKK03_09665"
     CDS             complement(4897..5568)
                     /locus_tag="PKK03_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNX84708.1"
                     /translation="MKLPGEIRYIDFHTHSQGGGGDTVAVRNLMAGDEVPSDFPANTL
                     FSAGIHPWQLTDDNLENLQTELLLTAAHPHVVMIGEAGFDRLRGAQGEVQYRAFLFQA
                     NIAGEMGKPVVIHCVRGWDELRRAHREVKPGRPWIIHGFRGSGSLAASLAEEGFWFSL
                     GAKGLTTEVLQSVYHEKLLLETDESGQSIAEVYRLFEAAAGYDEQRAESLVRNNFKLL
                     LNFNL"
     gene            complement(5577..7088)
                     /gene="gltX"
                     /locus_tag="PKK03_09670"
     CDS             complement(5577..7088)
                     /gene="gltX"
                     /locus_tag="PKK03_09670"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNX84709.1"
                     /translation="MADRKIRVRFAPSPTGPLHIGGVRTALYNYLFARHHGGTFILRI
                     EDTDQTRYVDGAEDYIMDSLEWAGIKVDEGVREGGPFAPYRQSDRKEIYHRYADELLR
                     SGDAYYAFDTPEELERLRQDHESRGETFNYGCTTRMSMCNSLTLPADEVEQRIASGEH
                     YVVRYRMPENEDIHFNDLIRGDIVVNTSTLDDKVLYKSDGMPTYHLANIVDDHLMEIS
                     HVIRGEEWLPSLPLHVMLYRSFGWQAPLFAHLPLLLKPTGKGKLSKRDGDKMGFPVFP
                     LYWPYGETASGYREEGYFPEAFVNMLALLGWNPGTEQEIFTMDELIAAFTIDRVGKSG
                     SRFDPDKAKWFNARYFQMKSNEELADLFMPLVNQKGFSPDRASLVSIVSLVKERAGFV
                     KDLWNESDFFFVAPESYDPEVVRKRWNADSPGQMGELRGKVAEVHHFAPEVLEQSVKE
                     WIASAGYNTGAILNLFRLLLVGASRGPHLFDIAAIIGREETLRRLDDGIRRLS"
     gene            7279..8313
                     /gene="tdh"
                     /locus_tag="PKK03_09675"
     CDS             7279..8313
                     /gene="tdh"
                     /locus_tag="PKK03_09675"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HNX84710.1"
                     /translation="MKKMKALVKAQAAKGLWMQEVPIPEPGYNDVIIKVKKSAICGTD
                     LHIYEWNEWAQKTIKVPMTIGHEYMGHVEKAGPGVTNVKVGDRVTGEGHLFCGTCRNC
                     RRGREHVCENTVGIGVHIDGSFAEYVKVPARNVVLLDSRIPDDWAAIMDPFGNATHTA
                     LAFPLLGEDVLITGSGLIGSMAVAIARFAGARHIVASDLSDYRLDLAKKMGATLVVNP
                     KKGEKIKDVLEPLNMKGFDVGLEMSGSPAAFREMIANMYNGSNIALLGILPSNFEVPW
                     SDIIFKALTLKGIYGREMWETWYKMQMMIIGGINLDPIITHRFPIDDFRKGFDIMEEG
                     NCGKVILSWD"
     gene            8502..>10125
                     /locus_tag="PKK03_09680"
     CDS             8502..>10125
                     /locus_tag="PKK03_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX84711.1"
                     /translation="MTLNPEHRSYEELSGNFTWAVAEKELDYKNGEVINIGWYCSDRI
                     CLKGNAAKVALIYEGFGGISRQFTYDDIRIAGNTIGTFLRGLGIIEGDRVCLFMDRIP
                     ELYLSFLGILKTGAIVQPLFSAFGEESLFVRLENAGTRAIITQKKHLQKVRKIRGKLP
                     HLEYVIIVDEIKSADLAAGEVLFRLDNAAPVENFDICPTMAETPSVLHYTSGTTGQPK
                     GVQHVHYSLISQYLTTKWVLDLRDDDIYWCTADPGWVTGTSYGIIGPWSLGVTQCVLD
                     SGFSAQAWYRFAEKNRVTVWYSAPTAIRALMKAGNEIVKSFDLSSLRHLASVGEPLNS
                     EAVIWSEKVFGKPFHDTFWQTETGSIMITNFPGMKIKPGSMGRTFPGITGTVLDPATF
                     RPYPEHGRAGLIAFRPGWPAMMRAYWNNEEKYREKFVNGWYLSGDRASIDSEGYYWFI
                     GRDDDVINTGGHLVSPFEVESALLEHPAVAESAVTSRPDEINMEVVKAFITLKPGHTP
                     DPDLELDIMNFIRKRLSPLAMPQAVEFMDRLPK"
BASE COUNT         2608 a         2697 c         2552 g         2268 t
ORIGIN      
        1 ccggaattga taaatcctga tttttttgat taacttacgg agtttcccgg caattcagga
       61 aataatgtca acactttaaa tattaacctt ttaaaatgaa tgattatggc agtacttaaa
      121 gttatcgaga ttcttgcatc atcaccggtc agctgggagg atgcaacagc ctctgccgtg
      181 aagatggcag caaaaacagt caaggagatc cgttctgttt atgtacagga ccagagcgct
      241 gtggtggtca agaacaagat caccgaatac cgtgttaatg taaaaataac ctttgaagta
      301 atttaattaa aaacagaatt atgggactta tttcatttat caagaatgca ggtgcaaaga
      361 tcttcaaatc agaagagaag cgcgaggagg aaaaggcaaa tatgattacc gagcatatca
      421 aaaagttcgg ttttgacgta agcagggtca gtgttgctgt cgatgacgaa aaggtggttc
      481 ttaccggatc ggttgacacc ctctcgaaca agaacaagat tatcgttacc gcaggtaacg
      541 ttgaaggcat ctcggtggtt gaagatcacc tcctggtcac aacacccgaa ccggttgctc
      601 ctcccgagcc ggagaagcag ttctacacgg tcaagaaagg tgattacctg tcgaagatcg
      661 ccaaggaggt ttacggtgac gcaaagaaat acccgatcat cttcgaagcc aacaaaccca
      721 tgctcaagga tcccgatctg atctatcccg ggcaggtcct ggttattcct ccactcactt
      781 aggaatgtct tgcatatgat atgtgcagag gcgggtctga agcccgcctc tgctgtttac
      841 ggcacaacat ttgatcaaaa ttgttgttat atttgcaccg gtgagaaaga tacgcatcat
      901 atcgccattc atatccctgt tcctgattac actcattctg tcaggcagtt tcggttttac
      961 ccttattcat catacctgta tccactgcgg aaccaacgag acaatcgcct ccctggccgg
     1021 tgacccggtc ggggataatt gctgcggcaa tcacggaaat tcctgctgcg gtgatcagaa
     1081 agcgacctcc tgcagccatg gagattgtga cgtacagcac cgccacagta caggtgacgc
     1141 ggttctttca gatgactgct gcacacacga gactgagagg gttgtgacgg acgaactcgt
     1201 gcgtacagag gcacagaatg agattcttcc ttacttcctt gcagcaacga tcattgctgt
     1261 catggaagaa catcaaacca ataactacaa tcatttttcg ggagagcagc ccttccactg
     1321 cgggcgcgac ctgacaacaa tgctctgccg tatccgctcc tgatactttc atagggaaac
     1381 aactgacacc ggaaaggatg actgccttac ccggatgttg ttgttgacgc tgaatgcgtg
     1441 atggttttca aaaaccgctg caataatgcc atttaattaa tttttaacat tttccctatg
     1501 aaacatttaa taacactgat aacaattctt cttatccctg ccttcgcggt ggcccagggt
     1561 cgcctggagg gcaaggtcgt agtcgccggc agtgacggtg cacccgacgg agaaggcctg
     1621 cccggtgcaa acgtggtctg ggccggaaca actgtcggag cttccgccaa tgcagcaggc
     1681 tattttacaa tcaggaaagt aaaggatcac aacctgcttg tggccagttt tgtcggttat
     1741 acaagtgaca ccgtcatgat tgatccaggc actgattaca tagagatcat cctaaaggaa
     1801 aacagcatga ttgatgagat cacggtaata ggccgggcca caggtacaca cattgaccgc
     1861 gaagccacaa ttgccacagt aaacatcact gccgcagagc tgtgcaaggc ggcatgctgc
     1921 aacctctcgg aaagttttgt caccaacgct tccgttgatg tcaactatgc cgatgccgcc
     1981 accggcgcga aacagataca gcttctcggc cttgccggct catacgttca gatactgaca
     2041 gagaacatcc cggcaatgta cggactggga gcggcttacg ggctgagcta tatccccggc
     2101 ccgtggatgg agtccataca ggtctcaaag ggcacctcct cggtgcgaaa cggaaacgag
     2161 tcaatcaccg ggcagattaa cgtcgaatac aagaaacctg caacaagtga gatagtattt
     2221 gtaaatggat tcgttagcga tgcaggcaag atcgagacca atgcaaatgc ttcactcagg
     2281 cttaaccaat actggagcac gatgctgctt ggtcacggcg aaacacagtc gggggccagc
     2341 gaccacaaca aagacggttt ccgcgatgag cctgacgtca ggcaatacag tttcatgaac
     2401 cggtgggact acctcagcaa taatctcacc ttccgcgcag gcatcaagta catgaatgag
     2461 gagaggattg gcggccagtt tgactatagc cgcaatgacc caaacacctg ggacagcggc
     2521 tacgggataa acatggctac agagagggct gaggccttca ccaagctggg aggcatgttc
     2581 ggcccctcag acaatatgtc tttaggctgg atacagagtt tcacctttca tgagatgaac
     2641 tcctggctgg gatacaacaa gtacgacgca acccagaaga gctattacac caaccttcta
     2701 taccagtact tcccggcaga gaaacacact gttgatgcag gtgtcagcta ccgcttcgac
     2761 cattatgacg agttgcaggg tatctacggg ccgctgaccg ggcaggtcct gaccaacatc
     2821 aatgcggaaa cagtcgaatc ggtaccggga atcttcatgc aatacaccta cactgattcc
     2881 gcaaaagtaa cactgctggc cggagtcagg tacgacttct accgtaacca cggcgcacag
     2941 ttcaccccgc gccttcacct gcgctatgaa atcacacccc atatgaccct cagggcttcc
     3001 gcaggaaaag gctaccggac agtgcatgta ctggctgaga atatgtactt cctgtcaagc
     3061 agcaggcaga tggtgattgc agacaacctc gacattgaag aggcatggaa cgcagggatc
     3121 aacctgacag ggtacatcca catcggcgac cgtgagctca ggcttaccgg cgaagcctac
     3181 cgaaccagct tcataaagca gatagtgact gatctcgact ctgacgttga cagggtatcg
     3241 ttttacaacc tcgacggaaa atcatattca aatgttatcc agctcgaggc acagatgcaa
     3301 cctgtgacag gactggatat cacagccgca tggcgctgga atgacgtcag gcagaccatc
     3361 ggcggagatc ttcgcgagaa gcctcttgcg tcacgctaca agggactgct cactgcctca
     3421 tacacaacac gcatgaggaa gtggcaggct gactatacac tgcagtacaa cggtcccgga
     3481 aggttaccct ccaccatggc aaatccggaa ccctaccgga ggcatgatca gttcggcgcc
     3541 tacccggtga tgaatgccca ggtgacgagg tatttcaaaa aatggagcgt ctatatcggt
     3601 gccgagaaca tcaccgggtc aaggcagcat gatgcgatca tcgcagccga tgatcctttt
     3661 ggcccgtatt ttgacggttc aatgatctac ggtccggtgc acggccggaa actctatgca
     3721 ggtttcagat ttgcactcga cagaccacag aattaaaaac aatactcaaa atacacaata
     3781 caatgaaaag caatcttata atcacagcag ttcttctctt cctcttctcc tcactcacgc
     3841 tcagcgccca ggagaaaaac aaaaaggatg tctcaacggt caggttcgcg acaagcatcg
     3901 attgtcagaa ctgtgtcaac acaatcatga agaacgtccc ctttgaaaaa ggcgtgaaag
     3961 acgtgaaatg tgatctcgca acaaaagagg tcaccgtgca gtaccagaag gataagatct
     4021 ccccggaaac gctgaagaga tcgattgaaa agcttggctt caccgcaaag gaggtgactg
     4081 aaaagaaaaa ggaggaagtg accccgcagg gaagcaggca gtaatggaaa aattaaactt
     4141 ttcctgtgtc cggacggata tccgtttaaa cagccccggt tataccggcg agttccctga
     4201 tgaccaccga ggcgcaggct atgccgaaag ctgcgggcat atatgatatc gttccgacgc
     4261 tcgatgcctt gttgctctca tcagagcccc tgacaatgag gttcttgtca accgcctccg
     4321 ggctgtaaac ggcagtgaat ccctcatgca cacccagctt gtgcagcttc ttgcgaagca
     4381 tgcgtgccag gttgcagaaa ttcgtttcag agatgtcagc tacccttatt gccgtcggat
     4441 catacttgcc tccggcaccc atggagctta ccaccctcag gttcagcctg agagagtgat
     4501 agacgagaaa aaccttgggt gacagagtgt ctatggcgtc aaccacatag ttgtattttg
     4561 cttcttccag gatgtcaatc atcctctgat cgcgaatgaa ctcggcaatg atccggagcc
     4621 gcaggtcggg gttgatgtca agcagcctct ctcccatcac ctcggctttc tttctgcccg
     4681 tggttgaggt aagcgccggc agttgcctgt tcctgtttgc agccgtaacc acatcaccgt
     4741 caatgattgt catggcgcct gcccccgccc tgcaaatcat ctcagcggcc catgatccca
     4801 cgcctcccag acctgccacc agcacatgcg aatccttcag tctctgcagt gcctcagggc
     4861 cgagcagcat ctcggttcgt gacagccatc cttccatcac aggttgaagt tcagtaataa
     4921 tttgaaatta ttccgtacca ggctttccgc cctctgctca tcataccccg cggcagcctc
     4981 aaagaggcgg tatacctcag ctattgactg acccgattcg tcagtctcga gaagcagttt
     5041 ttcgtggtat actgactgaa gcacctcggt tgtcaatccc tttgccccca gtgagaacca
     5101 gaaaccctct tcggccagtg atgctgccag gctgccgctg ccccggaatc catggatgat
     5161 ccatgggcgg ccgggtttga cctccctgtg cgcacgcctc agttcatccc accccctgac
     5221 gcaatggatc accaccggct tccccatctc cccggcaatg ttcgcctgga aaaggaaggc
     5281 acggtattgt acctccccct gcgccccgcg aagccggtca aacccggcct caccaatcat
     5341 caccacatga gggtgcgcgg cagttaacaa caactctgtc tgtaagttct ccaggttgtc
     5401 atctgtcagc tgccacggat ggatgcctgc agaaaagagg gtgtttgcgg gaaaatccga
     5461 aggcacctca tcacctgcca tcaggttcct gacagcaacg gtgtcacctc ctccgccctg
     5521 tgaatgggtg tgaaagtcaa tatatcttat ctccccgggg agtttcatca ggtttattat
     5581 gagagccgtc tgatgccgtc atcaagccgg cgcagcgtct cctccctccc tatgatggca
     5641 gcaatgtcaa agaggtgcgg gcccctggaa gcgcccacca gcagcagccg gaagagattg
     5701 agtatcgccc ctgtgttgta acccgccgag gcaatccatt ccttcacgct ctgctccagc
     5761 acctccggtg cgaagtgatg cacttcagca accttcccgc gaagttcacc catctgcccc
     5821 ggcgagtcag cattccagcg cttcctgaca acttcaggat catagctctc cggggccacg
     5881 aagaagaagt ccgactcatt ccagagatcc tttacaaacc cggctctctc cttcaccagt
     5941 gatacgatac tgacgaggga ggcccggtct gggctgaatc ccttctgatt caccaaaggc
     6001 atgaagaggt cagccagttc ctcattgctt ttcatctgga aataccgggc attaaaccac
     6061 ttcgccttgt cagggtcaaa gcgcgatcct gactttccga ccctgtcaat ggtgaaggct
     6121 gcaatcagct catccattgt gaatatctcc tgttccgttc ccgggttcca tcccaggagg
     6181 gccagcatgt tgacaaacgc ttcggggaag tagccctcct cgcggtatcc ggaggctgtt
     6241 tcgccgtatg gccagtaaag cggaaaaacc gggaatccca tcttgtcgcc gtcgcgtttg
     6301 ctcagcttac ccttgcctgt aggcttgagc aacagcggca ggtgcgcaaa gagcggagcc
     6361 tgccagccga agctgcggta aagcatcacg tgcagcggca gtgacggaag ccattcctcg
     6421 cccctgatca cgtgcgagat ctccatcagg tgatcatcga caatgtttgc cagatggtaa
     6481 gttggcatgc cgtcagactt gtacagcacc ttgtcatcga gggtggaggt gttgaccacg
     6541 atgtcacccc tgatcaggtc attgaagtga atgtcttcgt tctcaggcat gcggtatctt
     6601 accacataat gctccccgga agcaatgcgt tgctcaacct catctgcagg gagggtaagg
     6661 gaattgcaca tactcatcct ggtggtgcag ccgtagttga atgtctcacc acgtgattca
     6721 tgatcctgcc tcagcctctc aagctcttcg ggcgtgtcga aagcgtaata ggcatcgcca
     6781 cttctgagaa gctcatcggc atagcggtgg tatatctcct tcctgtcgct ctgccggtaa
     6841 ggggcaaacg gaccgccctc gcgcaccccc tcatcaacct taatgcccgc ccattccagt
     6901 gagtccatta tatagtcttc tgcaccgtca acgtaacgtg tctggtcggt gtcttctatc
     6961 cggaggatga aggtaccgcc atgatgcctt gcaaagagat agttataaag tgccgtgcgt
     7021 acgccgccga tgtgaagggg gcctgtgggg ctcggggcaa agcgcaccct gatttttctg
     7081 tctgccatat gctgaatgtt gcctgaactg atgatagccg ctccgctccg tcctgcaaag
     7141 gctcctgcgg cagcgcctga caaagataaa tattatgtgg ctaagggcaa tttacccccg
     7201 gggtcaacat taaaaatcat gttttgtgac ctctatttgt attattttta cgtcataacg
     7261 aattaatcat cactcacgat gaaaaagatg aaagcactcg tcaaggcaca ggctgccaaa
     7321 ggattatgga tgcaggaagt acccatcccc gagccgggtt acaatgatgt cataataaag
     7381 gtcaaaaaat cagccatctg cggtactgac cttcacatat atgagtggaa cgaatgggcg
     7441 caaaagacaa taaaagtacc catgacaatc ggccatgagt acatgggtca tgttgaaaaa
     7501 gccggcccgg gcgtcacaaa tgtaaaggtg ggtgacaggg tcaccggcga ggggcatctc
     7561 ttctgcggca cctgccgtaa ctgccgccgc ggccgcgaac atgtatgcga aaacaccgtc
     7621 ggcatcggcg ttcacataga cggctccttt gctgaatacg tgaaggtgcc ggcccggaat
     7681 gtggtgcttc ttgattcaag gataccggat gactgggcag ccatcatgga tccgtttggc
     7741 aacgccaccc atacagccct ggcgttcccg ctgctgggcg aggatgtgct catcacaggg
     7801 tcaggactga taggcagcat ggccgttgcc attgccagat ttgccggtgc ccggcatatt
     7861 gtcgccagtg acctgagcga ctaccgcctt gatctggcaa aaaagatggg cgccacattg
     7921 gttgtcaacc cgaaaaaggg agaaaagata aaggatgtac tggagccgct caatatgaaa
     7981 ggcttcgacg tggggcttga gatgtcaggg tcaccggcag ccttccgtga aatgatcgcc
     8041 aacatgtaca acggttcaaa tatcgccctg ctgggcatac tcccctcgaa ctttgaggtg
     8101 ccatggagtg atatcatatt caaggctttg acactcaagg gtatttacgg ccgtgagatg
     8161 tgggagacat ggtacaagat gcagatgatg ataatcggcg gcatcaatct tgaccccatc
     8221 attacccacc gattcccgat tgatgacttc cggaagggat ttgacatcat ggaggagggc
     8281 aactgcggca aggtgattct gagctgggac taatccgcag catgtatctg caatcatgcc
     8341 aacgtggtca atcatgacat tgctcatcat cccgtgagag gaaaagtcca tggccacagg
     8401 gtcagggatt tccgcttccc cataaatatg agaaaagtca gttctggagc ttgacgatta
     8461 cgaaaataat tttatcttac atgtcattgt tacgataaca tatgacgtta aacccggaac
     8521 acaggtccta tgaagaattg tcaggcaact tcacctgggc tgttgctgaa aaagagcttg
     8581 actacaaaaa cggtgaagta atcaacatcg ggtggtattg cagcgacagg atatgcctga
     8641 agggcaatgc agccaaagtt gccctgatct acgaagggtt cggtggaata agcaggcagt
     8701 tcacctatga tgacatccgt attgcaggca acacaatagg gacattcctc agaggtcttg
     8761 gcatcattga aggtgacagg gtatgcctct tcatggacag gatccccgag ctgtacctct
     8821 ccttcctcgg catactcaag accggcgcta ttgtacagcc gctcttttcc gccttcggcg
     8881 aagaatcact gtttgtcagg ctggaaaacg cagggaccag ggctataatc acgcaaaaaa
     8941 aacatctcca gaaagtcagg aagatacggg ggaagctgcc tcacctggag tatgtcatca
     9001 tcgttgatga gattaaatct gcagaccttg ccgccggtga ggttcttttc aggctcgaca
     9061 acgcagcgcc ggtggaaaac tttgacatct gccccaccat ggccgagaca ccttcagtgc
     9121 tgcattatac atcaggaacc accggccagc ccaagggggt gcaacatgtt cattactcac
     9181 ttatttcaca gtacctgaca acaaagtggg ttcttgacct tcgtgatgac gatatttact
     9241 ggtgcaccgc agacccggga tgggtcaccg gcacctcgta cggcatcatc ggtccgtgga
     9301 gcctcggagt gacacagtgc gtcctcgaca gcggtttctc ggcacaggca tggtaccgct
     9361 ttgccgaaaa gaacagggtt accgtatggt actccgctcc cactgcaata agggcactta
     9421 tgaaggccgg aaatgaaata gtgaagtcgt ttgacctgtc atcgcttcgc cacctggcaa
     9481 gtgtgggtga gccactcaac tcagaagccg tgatatggtc ggagaaagtg ttcggcaaac
     9541 cattccatga tacattctgg cagacagaga ccgggtcaat aatgataacc aacttcccgg
     9601 ggatgaagat taaaccggga tcaatgggca gaacatttcc cgggataacc ggaacggtac
     9661 ttgatccggc cacattccgg ccttatcctg aacacggaag agccggcctc atcgccttca
     9721 gaccagggtg gcccgccatg atgcgtgcat actggaacaa cgaggaaaaa tacagggaga
     9781 agtttgtcaa cggatggtat ctttcaggcg acagggccag cattgacagc gagggatatt
     9841 actggttcat cggccgtgat gatgatgtca taaatacagg cgggcacctt gtgagtccct
     9901 tcgaggttga atcggcactg cttgaacatc cggccgtggc cgaatcagcc gtcacctcca
     9961 gacctgatga gatcaacatg gaggtggtca aggcgttcat taccctgaaa cccggccata
    10021 cacctgaccc agacctcgaa cttgatatca tgaacttcat caggaaacgt ctgtctcccc
    10081 ttgccatgcc gcaggccgtg gaattcatgg acaggctccc gaaaa
//
LOCUS       DAOPXU010000101        10064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_7827_length_10064_cov_9.452693, whole genome shotgun sequence.
ACCESSION   DAOPXU010000101 DAOPXU010000000
VERSION     DAOPXU010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10064
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7827_length_10064_cov_9.452693"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="PKK03_09685"
     CDS             <1..1353
                     /locus_tag="PKK03_09685"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNX84712.1"
                     /translation="LKMLSHPLSARRGNLIAAAGMGLAIVATLLFHRKEGEPIGNIGW
                     ILAAMAAGTVIGWMIAKKVKMTAMPQLVSFFNGMGGAAAALISLMEFPHVSQELMMKS
                     GMANGHVLAILLGLVIGTVSWAGSMIAFGKLDGRIGDLRVRAMKYVNLTILAAIIGFI
                     VFIMTREVTASAELIHLVIILFGVAIIYGVLFVMPIGGADMPVVISLLNSFTGVAAAM
                     GGFLYNNQAMLTGGILVGSAGTILTVLMCRAMNRSLLNVIIGVFGGGGATTATAAGSI
                     KEVSVTDAAVLLSYSKKVVIVPGYGLAVAQAQHLCHELDKLLDEKGVEVKYAIHPVAG
                     RMPGHMNVLLAEADVPYEQLVESDEINPDMPNTDVIVVIGANDVVNPAAEDDTSSPIY
                     GMPIVRAREAKNIIVMKRGMGKGYAAIDNMLFFNDKTRMLFGDAKSTLQALINEVKAL
                     "
     gene            complement(1377..2483)
                     /locus_tag="PKK03_09690"
     CDS             complement(1377..2483)
                     /locus_tag="PKK03_09690"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNX84713.1"
                     /translation="MKRFFLLIIVAFLSVSLFSQDFFRGDFSKDKKYVILVNPTAGNI
                     GVVTFLMDNGILKTDPGLVDFVGVYHSSQEYDFSKSAEYIAANGLTRFHLHEVNAPLT
                     EEMVYRQNPCSDDFRKIFSNSVGIIFFGGQDIPPAIYGQENWYSETTDPGRHYFEVSF
                     LFHLLGGTRNSSYRPILEDNPDYMVTGFCLGLQSMNVAAGGTLWQDIPAQVYQSTKPE
                     TNVLIDRKNLHRNYWQNIRDDKDFMGISMHPVRFTENMFFGKTVKVSRKLQPIVYSSH
                     HQAINELAPVFNVTARSNDGKIIEGIAHKKYPNVFSVQFHPEVSALYEDRALVKFAPD
                     DTPASLHSMLDKKSLKFHLKYWGHISDVILERSR"
     gene            complement(2514..3977)
                     /locus_tag="PKK03_09695"
     CDS             complement(2514..3977)
                     /locus_tag="PKK03_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84714.1"
                     /translation="MKTVILAASLIGLFCTSCNNSPDNIRDAILNHKNSFYYINFRSY
                     PSNDESLPVGVFDSGTGGLSVLADIVQHDGLAAESYIYMGDLANMPYGSYSLENNTDL
                     LIEHVMKDVQFLLGNKYYRSGNATTPETDKKPVKAIVIACNTATAYALDTVKRFLREA
                     GSDIGVIGVIDAGARGAFGNFTPGENGSVAVMATDGTVKSGAYPQAIMLVNREDGQEE
                     KIAVFQQAGIGIAEAIDGSPDAIDMKATAPRADYKGPSENGSGDMKIDQELLERYDFD
                     LSNGAILFEGDAENPRNIQINSVENYIAFHVVSLMEKIRKTEGALPLGSVVLACTHYP
                     FFTEIFRNEFSRLRDYRENGEYIYRSLIAEDLILVNPAVEVADQLYEHLASRNLLYEG
                     TGEGSEFYISVPSLTNKQVKLRDDGSFTYDYKYGRKAGEIQEYVKVVPFSRTSIPEDV
                     LNRLSVQIPGVWALITEFNSASPKAGYLSPSEKITVE"
     gene            4221..4541
                     /locus_tag="PKK03_09700"
     CDS             4221..4541
                     /locus_tag="PKK03_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNX84715.1"
                     /translation="MEAGKAKSRKYYTDDQEKLSRYAKALSHPARVFVMDFLANNLDR
                     CCYSGDMAEELPIARSTLSEHLRELKKAGLIQGEINPPYIKYCINREKWDEAKVLLNR
                     FFKR"
     gene            4592..4825
                     /locus_tag="PKK03_09705"
     CDS             4592..4825
                     /locus_tag="PKK03_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNX84716.1"
                     /translation="MEIKILGPGCPKCKTLEKLTREVVEKSGIDANVTKVDDIVAIMN
                     YGVMTTPALVIDEKVVVKGRVPSAEEILKLITT"
     gene            4838..5290
                     /locus_tag="PKK03_09710"
     CDS             4838..5290
                     /locus_tag="PKK03_09710"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HNX84717.1"
                     /translation="MKKLFPVLAVVVIITAAIAIFAFRGSESQVAGDTSGISTDKVEV
                     IYFHLTRRCVTCQAVENVARDAVKELYPAEVEKGTVVFRSLNIEDKSSEADAQRVNAT
                     GQCLLIVSGDTRIDLTSEGFMNARNSPGKLKEEIKKAVDPLLKALTSS"
     gene            5290..5991
                     /locus_tag="PKK03_09715"
     CDS             5290..5991
                     /locus_tag="PKK03_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HNX84718.1"
                     /translation="MEILQKILENSQYSFLTAAILGLMTAISPCPLATNISAIGYISR
                     DLDDRRRVFLNGLVYTLGRAITYAGIALIIFFGASQMDISGWFQRWGEKILGPVLIII
                     GFFMLDFIRFKLPGSVGSLTERIGEKGRKSYLNTLLLGILFAVAFCPYSGVLYFAMLI
                     PMTVASAEGLYLPLVFAVATGIPVIIFAWLLAYAVGNVGKVYSRLKTFELWFRRVVAV
                     LFIGVGIWYVLSVWF"
     gene            6045..7388
                     /locus_tag="PKK03_09720"
     CDS             6045..7388
                     /locus_tag="PKK03_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNX84719.1"
                     /translation="MESRKEIKILLWIIAVFIFAFFMPIGSVRFNTAIAAAFDLVKWY
                     AREHVILCLLPAFFIAGVISVFVSQGSVIKYFGANAKKWLAYSVAAVSGTILAVCSCT
                     ILPLFSSIHKRGAGLGPAIAFLYSGPAINILAIILTARILGFEMGVARVIGAVLFSIV
                     IGLIMSVIYRKEEMQKKEDQMHIVAAPEKRPMWQTMTHFFTLVLILVFANWGSPAADD
                     TSSAWYYIFSYKWYITGFFGLVLAWSLIKILKIKWQWVLAALVITVLSVFLTNSFIES
                     SKLKPLIPMVVGIAALSVITLYDKRDKENREWTLSTWGFARQIIPLLGIGVVIAGFLL
                     GSTHDGQSIAGVIPNEWIAKLVGGNSVFSNFFASVVGAFMYFATLTEVPILQGLIASG
                     MGKGPALALLLAGPSLSLPNMLVIKDIIGFQKTAVYVGLVVVMATISGLVYGAIL"
     gene            complement(7441..7776)
                     /locus_tag="PKK03_09725"
     CDS             complement(7441..7776)
                     /locus_tag="PKK03_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84720.1"
                     /translation="MKKFIPGISALIILSFVVISVVNAQNDQKKEKKAKTESVKDCSK
                     CPSAASCEMAAGEAPVPGSQVKHASANCDPAKCKEGKCDAACKTACTKTGPDAKCDPA
                     KCHSVAEKK"
     gene            7907..9169
                     /locus_tag="PKK03_09730"
     CDS             7907..9169
                     /locus_tag="PKK03_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNX84721.1"
                     /translation="MKRSRAQLMILLLALGALIALTAIQFSWILKAARMQEAQFNHSV
                     EMAMMRIVETLSGNEAICSEVNNCLKHGGNESCMLVMKTREEWESMKSLIRKDLNFYG
                     IGLDFEFDIVSSDLPANIPRPKNTYFSNGLEEMLEKSGYRLSLRFPEKRDFIIAQMGD
                     IFIFSLLLLFLVTISFIIIYNFYRREKLLSESIVDFVNNVTHEFKTPLTNISLANSML
                     SKNEKVESDEKLSFYSSVIRTEQAKLNEKVEKLLKTDFAVIDKSQTLEEINAGAAVED
                     IAETFRVQIEQKGGKLDIEKEGRRFTVTGNPDLFYIALGNLVDNAVKYSGQPPEILVK
                     LISKDNRLLIEINDKGPGIAREFRDKVFEKYFRVPAGNTHNVDGFGLGLYQVRGIIAG
                     MKGNIKISNRKEGGLAVMIDLPLAPGNE"
     gene            9174..9875
                     /locus_tag="PKK03_09735"
     CDS             9174..9875
                     /locus_tag="PKK03_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX84722.1"
                     /translation="MKNTRILLAEDDVNLGFLLVDFLESNGFEVILYRDGLSALQGFS
                     RAGADFCLIDIMLPGMDGFTLTSGIKKLDSRIPVIILSARSLKEDKIAGFRLGVDDYI
                     TKPFEEEELLYRIRAVLNRTIQSGPVAFQTFDVISIGQFSFDPQNLMLTHGGNTQRMT
                     IKECKILKMLAVSVNNIVTRDEIMIGVWGEADYYTGRSLDVFISKLRSYLKRDPSVRI
                     VTIPTLGYKLEIEKV"
BASE COUNT         2519 a         2298 c         2578 g         2669 t
ORIGIN      
        1 ctgaagatgc tcagtcatcc actgtcggca cgcaggggaa accttatcgc tgcggcaggt
       61 atgggactgg ccatagtagc caccctcttg tttcaccgga aggaaggtga acctatcggc
      121 aacatcggct ggatacttgc cgccatggca gcgggcacgg tcatcggatg gatgatagca
      181 aaaaaggtga aaatgaccgc aatgccgcag ctggtttcct tctttaacgg tatgggcggc
      241 gcggcagccg cactgatctc cttgatggaa ttcccgcacg tgagccagga actgatgatg
      301 aagtcaggca tggccaacgg ccatgttctg gccatccttc tcggcctggt gatcggaacg
      361 gtatcctggg ccggaagcat gattgcattc ggcaaactcg acggccggat tggcgacctg
      421 agggtcaggg ccatgaagta tgtaaacctt accattcttg cagccattat cggattcatt
      481 gtctttatca tgacccgtga agtgacggca tccgctgaac tgatacacct tgtgatcata
      541 ctgttcggtg ttgcaataat ctatggtgtg ctgtttgtca tgccaatagg cggggccgat
      601 atgcccgtgg tcatttcact tcttaacagc ttcaccggcg ttgcagccgc catgggcgga
      661 ttcctgtaca acaaccaggc gatgctcacc ggcggtatac ttgtcgggtc ggccggaacc
      721 atcctgacag tgcttatgtg cagggcaatg aaccggtcgc tgctgaacgt catcattggt
      781 gtgttcggcg gtggcggggc aaccactgca accgctgcgg gcagcataaa ggaggtgtcg
      841 gttactgatg ctgccgtact gctgagctat tccaaaaaag tggtcattgt ccccggctac
      901 ggtctggcag tggcacaggc acagcatctg tgccatgagc ttgacaagct cctcgacgaa
      961 aagggggtgg aggtgaaata tgccattcac ccggtggccg gacgcatgcc cggtcatatg
     1021 aacgtacttc ttgccgaggc tgatgtaccg tatgaacaac tggtcgagag tgatgagatc
     1081 aaccccgata tgcccaacac cgacgtcatc gtagtcatag gagccaacga tgttgtcaac
     1141 ccggcagccg aggatgatac ttcgagcccg atatacggaa tgccgattgt cagggcccgc
     1201 gaagcaaaaa atatcatcgt aatgaaacgc ggtatgggga agggttatgc tgccatagac
     1261 aacatgcttt tcttcaatga caaaacccgg atgctctttg gtgacgccaa atcaacactg
     1321 caggcactga tcaatgaggt gaaagcccta tagatcagtc ggcagcgtgc tgacttttat
     1381 cttgaacgtt ccagaattac atcagaaatg tgtccccaat acttcagatg gaacttcagg
     1441 ctctttttgt caagcattga gtgcaggctg gcaggggtgt catcaggagc aaatttcacc
     1501 agggcacgat cctcgtacag ggctgagacc tccgggtgaa actgaacgga gaagacgttt
     1561 ggatactttt tatgtgcaat tccctcaatt attttgccgt cgtttgacct tgccgtcaca
     1621 ttgaataccg gcgccagctc atttattgcc tgatgatggc tgctgtagac tattggctgc
     1681 agcttgcgtg aaacctttac tgtcttcccg aagaacatat tttcagtaaa cctcacagga
     1741 tgcatgctta ttcccataaa gtccttgtcg tccctgatgt tctgccagta attgcggtgc
     1801 aggttcttgc ggtcaatcag cacattcgtc tcgggctttg tgctctggta tacctgcgcg
     1861 gggatatcct gccatagggt tcctccggct gcaacgttca tcgactgcag gcctaagcag
     1921 aagccggtaa ccatatagtc cggattgtct tccagaatgg gtctgtagct tgaatttcgt
     1981 gtaccaccca acaggtgaaa caggaagctc acttcgaagt agtgccttcc cggatcggtg
     2041 gtctccgaat accagttctc ctgcccgtag atggccgggg gtatgtcctg tccgccaaag
     2101 aatatgattc ccactgaatt gctgaatatc ttccggaagt cgtcactgca cggattctgc
     2161 ctgtaaacca tctcttcggt aagtggtgcg ttcacttcgt gcaggtggaa gcgggtcagt
     2221 ccattggcgg caatatactc agcagatttg ctgaagtcat attcctggct ggagtgatat
     2281 accccgacga agtcaaccag tccggggtcg gttttcagga tgccattatc catcaggaat
     2341 gtcaccacgc caatattacc tgcggtggga ttgacaagga tgacatactt tttatccttg
     2401 ctgaaatcgc ccctgaagaa atcctgtgaa aagagagaga cagacaggaa ggcaacgatg
     2461 ataagcagaa agaacctttt catagcataa ttatttaaac acaaatgtaa tgtttattcg
     2521 actgtaatct tttcagaagg ggagaggtac ccggccttcg ggctggcgga gttgaattca
     2581 gtgatcagtg cccatacccc gggtatctga actgaaaggc ggttcagcac atcctccgga
     2641 attgatgttc tgctgaaggg taccaccttc acgtattcct gtatctcgcc tgccttgcgg
     2701 ccgtatttgt aatcgtaggt aaatgagccg tcatccctta gtttgacctg tttattggtc
     2761 aggctgggga cgctgatgta gaattcgctt ccttcgccgg taccttcgta cagcagattc
     2821 ctggaagcaa ggtgttcata gagctggtcc gcaacctcca cggcagggtt gaccaggatc
     2881 aggtcttcgg caataagcga cctgtagatg tactcaccgt tttcgcggta gtccctgagc
     2941 ctgctgaatt cgttccggaa gatctccgtg aagaacgggt agtgggtgca tgcaagaaca
     3001 accgagccaa ggggcagcgc tccttcggtc ttccttatct tttccataag tgagaccaca
     3061 tggaatgcga tatagttttc caccgagttg atctggatat tgcgtggatt ttcggcatca
     3121 ccttcaaaca gaatggcccc gttgctgagg tcaaaatcat acctttccag gagttcctga
     3181 tcaatcttca tgtcgccgga gccgttttcc gagggtccct tgtaatccgc ccgcggggcg
     3241 gtggccttca tgtctatggc atcgggactc ccgtcaattg cctcggcaat gccaatccct
     3301 gcctgctgga atacggctat cttctcctcc tggccgtcct ccctgtttac cagcataata
     3361 gcctgtgggt atgcgcctga tttcacggtg ccgtctgttg ccatcacggc gacgctgcca
     3421 ttctcgccgg gggtgaaatt cccaaatgct cccctggccc ccgcatctat cactccaata
     3481 acgcctatat cagaccctgc ctctctgagg aatctcttca ccgtgtccag tgcgtaggca
     3541 gtggccgtgt tgcaggcaat gactattgcc tttaccggct tcttgtcagt ctcgggggtg
     3601 gtggcattac ccgatcgata gtacttgttt cccagcagga actgcacatc cttcataaca
     3661 tgctcaataa gcagatccgt attgttctcg agcgagtagc ttccgtaggg catgttggcg
     3721 agatccccca tgtagatgta cgattcagct gcaagtccat catgttgaac gatatccgcc
     3781 agtaccgaga gtcctcccgt tcccgagtca aacaccccga cgggaaggga ttcgtcattc
     3841 gagggataac tcctgaaatt gatataataa aagctgtttt tatgattcag tatcgcatcc
     3901 ctgatattgt ccgggctgtt gttgcacgag gtgcagaaca ggccgatgag ggaagcggcc
     3961 aggattacgg tcttcatcag gacttttttc atgggtgcag taacatcact ggtttggtca
     4021 ctgtgaattt agcaccaaat ggggataaac gcaatcactt ctgctgaaga agtataaaaa
     4081 ggcaacccgg ggcagttcag caatcacatc cggacaacaa tacacagttg aataatcagc
     4141 agagattaat aattcttttc tgccaccata ccctgtattc aataaaacat tatttttgtt
     4201 tcgtcatttc ccgaaatgaa atggaggctg gaaaggcaaa aagtagaaaa tactatactg
     4261 atgatcagga aaagctgtca aggtatgcga aggccctgtc gcatcctgcc agggtattcg
     4321 tgatggattt tctcgctaac aatcttgaca ggtgttgtta cagtggtgat atggctgagg
     4381 aattgccgat tgccaggtca acactttctg agcatcttag ggagttgaag aaggccggtc
     4441 tcatacaggg cgagatcaat cctccctata tcaaatactg tattaaccgt gagaaatggg
     4501 atgaagctaa ggttctgctg aacaggtttt tcaaaagata aaaaatttaa aataacattt
     4561 cgacgtttta cgaaataagc gggataaaaa aatggaaatc aaaattctgg gccccgggtg
     4621 ccccaaatgc aaaaccctgg agaaactgac acgcgaggtg gttgaaaaga gcggtattga
     4681 tgccaatgtc acaaaagtgg atgatattgt tgcgatcatg aattacggag tgatgacgac
     4741 gccggcgctg gtcattgacg aaaaggttgt ggtgaaggga agggtaccat cggcggaaga
     4801 aatactgaaa ctgataacca cataataaag taccaatatg aaaaagttat ttccggttct
     4861 ggctgtcgta gtaataatta cggcagcaat agcaattttc gctttcaggg gaagtgagtc
     4921 acaggtggca ggggatacct ccgggatttc aacagataag gtagaagtga tttattttca
     4981 tctgacccgt cgatgtgtta cctgccaggc agttgagaac gttgccaggg atgccgtgaa
     5041 agagctctac ccggcagagg tcgaaaaggg aacggttgtt ttcaggtcgc tcaacattga
     5101 agacaagtcc tcggaggccg acgctcaaag agtaaacgcc accgggcagt gcctgctgat
     5161 tgtcagcgga gatacaagga ttgatcttac cagcgaaggc ttcatgaatg cacgtaacag
     5221 tccgggaaaa cttaaggaag agataaagaa agcggttgat ccgttgctta aggcattaac
     5281 aagcagctga tggagattct gcagaagata cttgagaact cacagtattc attcctgacg
     5341 gcggcaattc tgggattgat gactgccatc agtccctgtc cgctggcgac caacatatcg
     5401 gctattggct atatcagtcg tgatcttgac gacaggagga gggtcttcct caacggactt
     5461 gtctacaccc tggggcgggc gataacctat gcagggattg cacttataat cttcttcggt
     5521 gccagtcaga tggatatttc aggctggttc cagcgatggg gagagaaaat ccttggtccg
     5581 gtgcttataa ttattggttt cttcatgctg gatttcataa ggtttaagtt gccaggttca
     5641 gtgggttcac tcacggaaag aatcggagag aaggggcgga aaagctatct gaacacactg
     5701 cttctcggga tactgtttgc cgtggccttc tgcccctata gcggagttct ttatttcgca
     5761 atgcttatcc ctatgacggt ggcgagtgca gaaggccttt atctgccgct tgtatttgca
     5821 gtcgcaacag gtatcccggt gatcatcttt gcctggctgc tggcctatgc ggttggcaat
     5881 gtcggcaagg tttacagccg cctgaagaca tttgagctgt ggttcaggag ggttgtggct
     5941 gtactcttca tcggggtagg tatctggtat gttttgtcag tctggtttta aattttttaa
     6001 tcatatattt cgtgattctg cgaaataaaa gtatttaatc tgaaatggag tcaaggaagg
     6061 agataaagat cctgctgtgg atcattgcag tttttatatt tgcgtttttc atgccgatag
     6121 ggagcgtacg tttcaatact gcaattgcag cggctttcga tctggttaag tggtatgcaa
     6181 gggagcatgt tattctgtgc ctcctgccgg cctttttcat tgcaggggtc atttcggttt
     6241 ttgtcagtca gggctcggtt ataaagtatt tcggagccaa tgcgaaaaaa tggctggcct
     6301 actctgtagc tgccgtttca ggtactatac tggcggtatg ctcatgcaca atactaccgc
     6361 ttttctcaag cattcacaag cgtggggcag gccttggtcc ggccattgcg ttcctttact
     6421 ctgggccggc tatcaacata ctggcaatca tactcactgc ccgaattcta ggttttgaga
     6481 tgggcgttgc ccgggtcatc ggggccgttc tgttcagtat tgtgatagga ctgataatgt
     6541 cggtgatata tcggaaggag gaaatgcaga aaaaggagga tcagatgcat attgttgctg
     6601 ctccggagaa gagacccatg tggcagacga tgacacattt ctttaccctg gtgctgatcc
     6661 ttgtatttgc caactggggc agcccggctg ctgatgacac ctccagcgcc tggtattata
     6721 ttttttcata caaatggtat ataacgggat ttttcggcct ggttctggca tggtcactga
     6781 taaaaatcct taaaattaag tggcagtggg tactcgctgc tttggtaata acggttcttt
     6841 cggtgttttt gaccaactcc tttatcgaaa gctcgaagct gaaaccgctg ataccgatgg
     6901 tagtgggtat cgcagccctg agtgttatca ccctttacga caaaagagac aaggagaatc
     6961 gtgagtggac actctccaca tggggatttg ccaggcagat tatcccgcta ctgggaattg
     7021 gggttgtcat tgcaggattc ctgcttggat cgactcatga cggacagagc atcgccggcg
     7081 tcatcccgaa tgagtggatc gcgaagcttg tgggaggtaa ctcggtgttc tcaaatttct
     7141 ttgcttctgt ggttggcgca tttatgtatt tcgccactct gacagaggtt cccattttac
     7201 agggactgat agcatccgga atggggaaag gtccggcgtt ggcactactg ctggccggac
     7261 catcgctttc tttgcccaat atgcttgtga tcaaagatat tatcgggttt cagaagactg
     7321 ccgtatatgt gggtcttgta gttgtcatgg cgacaatctc aggactggtt tacggtgcaa
     7381 ttctctgagg caacggataa gcgaaacaga gggtaaggta tcaggcagaa tagccggact
     7441 ttactttttc tcagcaactg agtggcattt tgccgggtcg cattttgcat ccggtcctgt
     7501 cttggtgcag gcggtcttgc aggcagcatc acacttccct tctttgcatt ttgccggatc
     7561 acagtttgct gaagcatgct tcacctgaga cccgggtacc ggagcttcgc cggcggccat
     7621 ttcacagctg gctgcagagg ggcattttga acagtctttt acggattccg ttttggcttt
     7681 cttttccttc ttctggtcat tctgtgcatt tacaactgat atcacaacaa acgacagaat
     7741 tatgagagct gagattccag ggatgaattt cttcatgact ttaatagttt ttgtttgcgt
     7801 caaaattagg cagacggatt tttaatcagg ttatcctgtt gttaaatcgt gttaccccga
     7861 tgttaaatct taatcctcat cataaaaact ccttattttt gagcttatga aaagatcgcg
     7921 cgcacaactg atgattcttt tgctggcatt gggcgccctt atagccctta ccgccataca
     7981 gttctcatgg atcctgaaag cggcccgcat gcaggaagct cagtttaacc acagcgttga
     8041 gatggcaatg atgagaatcg tggagaccct gtcaggaaac gaagcaatat gcagcgaggt
     8101 aaacaattgc ctgaagcatg gcggcaatga gtcgtgcatg cttgtgatga agacaaggga
     8161 ggagtgggaa agcatgaaat cactgatcag gaaggacctg aacttctatg gaataggatt
     8221 ggactttgaa tttgatatcg tgagctcgga cctgcccgct aacatccccc ggcctaaaaa
     8281 cacctatttc agcaacggcc ttgaggagat gcttgaaaaa tccggttaca gactcagcct
     8341 ccgctttccc gagaaacggg acttcatcat cgcccagatg ggcgatatct ttatcttctc
     8401 gctgctcctc ctgtttcttg tcacaatctc gttcataatc atatataatt tctaccggag
     8461 agaaaagctc ctttcggaga gcattgtcga ttttgtaaac aatgttactc atgagttcaa
     8521 aactccgctg accaatatat cacttgccaa cagtatgctc tcaaagaatg aaaaagtgga
     8581 aagtgatgag aaactgtcgt tttactcttc tgtcatcaga accgaacagg ccaagctgaa
     8641 tgagaaggtg gagaagttgc ttaagactga ctttgcagta attgacaaat cccagaccct
     8701 ggaggagata aatgccggcg cagccgtgga agatattgct gagacattca gggtacagat
     8761 tgaacaaaaa ggcggaaaac tggacatcga aaaggagggc cgccggttca cggttaccgg
     8821 caacccggat ctgttttaca ttgccctcgg gaaccttgtc gataatgcag tcaaatattc
     8881 tggtcaacca cctgaaattc tcgtaaaact gatttctaag gataacaggc tgttgattga
     8941 gattaatgac aaaggtcccg gaatagccag ggaattccgg gataaagtat ttgagaagta
     9001 tttccgtgtt cctgcgggca atactcataa tgtggacggt ttcgggcttg gactttacca
     9061 ggtaagggga attattgcgg gaatgaaagg caatattaaa atttcaaaca ggaaagaagg
     9121 cggtcttgcc gtgatgattg accttcccct ggcaccggga aatgagtaac aacgtgaaaa
     9181 acacaagaat actccttgcc gaggatgacg tcaaccttgg atttttgctg gttgatttcc
     9241 ttgagtcaaa cggttttgaa gtgatactgt atcgtgatgg tctttcagca ttgcagggtt
     9301 tttcaagagc cggagccgac ttttgtctga ttgacatcat gctgcccggc atggatggtt
     9361 tcacactcac atccgggata aagaaacttg acagcaggat ccccgtgata atactctctg
     9421 cacgttcact caaggaagac aagatcgcgg gttttcgcct gggagtcgat gactatataa
     9481 ccaaaccgtt cgaagaggag gaacttcttt acagaattcg tgctgtgctc aacagaacga
     9541 tccaatcagg cccggttgca ttccagacat ttgacgtcat cagcatcggc cagttctctt
     9601 ttgatccgca gaaccttatg ctgactcatg gcggcaacac gcagagaatg acaataaagg
     9661 agtgcaaaat actcaaaatg cttgcagttt cggtaaacaa catcgttaca cgtgacgaga
     9721 ttatgatcgg cgtctggggc gaagctgatt attataccgg acggagtctc gacgtattca
     9781 tttccaaact gcgaagttat cttaagagag atccctctgt ccggattgtc accataccga
     9841 cacttggtta caagctcgaa attgagaaag tctgattgat gattttgatc cggatttgga
     9901 gaaattctta ttacaagaat ttcgtaaata tacgaaatga ttatctttga ttagtcagat
     9961 caggcaagag gcaattggct gcagcgtaag gcaatgataa gcaatatacg gaccttcaga
    10021 ccttcagacc ttcagacctt cagaccttca gaccttcaga cctt
//
LOCUS       DAOPXU010000102         9997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_7909_length_9997_cov_13.724502, whole genome shotgun sequence.
ACCESSION   DAOPXU010000102 DAOPXU010000000
VERSION     DAOPXU010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9997
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7909_length_9997_cov_13.724502"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..550)
                     /gene="rplU"
                     /locus_tag="PKK03_09740"
                     /pseudo
     CDS             complement(251..550)
                     /gene="rplU"
                     /locus_tag="PKK03_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
     gene            794..1399
                     /locus_tag="PKK03_09745"
     CDS             794..1399
                     /locus_tag="PKK03_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HNX84723.1"
                     /translation="MQNKLIRLKVIGISYSQTQSGAYALILAEENGERRIPIIIGGFE
                     AQAIVIKLENLEPPRPLTHDLFRSFAEACGVTVEQVLINRLREGIFYSEIVCTDGERR
                     FHIDSRTSDAVALALRFECPIFIEPQILNEAGIVMSATTEEEQKKQAEPPKEKKQPVS
                     SEYSEFSVDELKDMLSDAIGREDYEQAAAIRDEMARRKGQG"
     gene            1414..3369
                     /locus_tag="PKK03_09750"
     CDS             1414..3369
                     /locus_tag="PKK03_09750"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HNX84724.1"
                     /translation="MKILRILIAAGLLIPVPLLSKEYKVKSPDGKITLSAETTTGINL
                     TVSYENKEIITGLSPELQLEGVTIPGQRPAIAKAVTSSVNNILVPVVPHKNSRVPDIF
                     NALELRFRGGSSIELRVYDDGVAYRFKSAMKGTVKVRDERFAFSLPGGAGAFWPLEKG
                     FMSHNECTFFPASMDTIGPKHLASLPALFTSGPINILLSEADIHDYPGLWINGDGRGG
                     VKGVLPAYPAAVKAHNDRNVYVTGREDYLAVTSGTRTYPWRLFIITPDDAGLVESEMV
                     YRLGAPPASGADFSWVKPGKVAWDWYNANNIYGVDFRAGLNNDTYRYYIDFASENGIE
                     YVILDEGWYKLGDVLEQAPGFNVGELCEYAASKNVGIILWVVWKTFHDRIDEALAQFE
                     KWGVKGIKVDFMQRDDQWMVNYYYEVAEKAARHKMLVDFHGSYKPDGMERTWPNVITR
                     EGVKGLENDKWSADCNPEHDLTLPFTRMVAGPMDYTPGAMVNMQQRDFKPIYYRPASQ
                     GTRVHQMAMYVVFESPLQMLADSPSNYKRNQECTSFIAGVPVTWDETRVVEARKGDNI
                     VIARRHGTVWYLAAMNDWKPFATEVDLSFLPSGEYSMEIFSDGINADRHGEDYKHVTR
                     RAAAGEKITLTLAPGGGWVAKITPSVR"
     gene            3385..4179
                     /locus_tag="PKK03_09755"
     CDS             3385..4179
                     /locus_tag="PKK03_09755"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNX84725.1"
                     /translation="MKQYLDLLSHVMEHGIEKRDRTGTGTVSTFGYQMRFDLAERFPL
                     MTTKKLHLKSIIHELLWFLSGSTNVRYLQENGVRIWNEWADADGNLGPIYGYQWRSWP
                     VTDGRTIDQVKTVVTSLKNNPDSRRHIISAWNVGEIEKMALPPCHILFQFYVAGDRLS
                     CQLYQRSADIFLGVPFNIASYSLLTMMMAQVTGYRPGEFIHTLGDAHIYLNHIDQVKL
                     QLTRDPRRLPVMSLNPDVRDILGFTYADFSLSEYDPHPAIKGDISV"
     gene            4181..4669
                     /locus_tag="PKK03_09760"
     CDS             4181..4669
                     /locus_tag="PKK03_09760"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNX84726.1"
                     /translation="MITIIVATDRNNGIGYNNSLLAHIPGDLRRFKDITMGHCLIMGK
                     KTWESLPNKPLTGRKNIVLTDNELDCFNCAETARSIDEALSLCDPGREIFIMGGGSVY
                     RQFLPLADRLMVTHIHWEYQADTFFPEISPDEWYVSEQEDFLSEDPSEISFSYTTYLR
                     RR"
     gene            4758..5606
                     /locus_tag="PKK03_09765"
     CDS             4758..5606
                     /locus_tag="PKK03_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84727.1"
                     /translation="MKIKTLILFTALTGLIMTSCSKSDPIDTKSTDAADDVALSEALY
                     DDAFASLEIATVFAEAGKKSAYVLDSCPLITINSPGDEFFPRNIVIDYGTGCTGLFDI
                     ERSGKILITLSGPRGTTGTERVLTFDNYYVNGAKVEGTFSIENLGPNNNQNVVFGVSL
                     AGGKITFPDSKVITREFTRQREYIAGYGTWNPWDDRCLITGMSAGTNLDGKTFALTIT
                     SPLEWTAACRFLVTGTIGFDVEGIEPFVLDYGTGECDALATLSRGDETREITLRYWHP
                     KYQGNQ"
     gene            complement(5714..6928)
                     /gene="clpX"
                     /locus_tag="PKK03_09770"
     CDS             complement(5714..6928)
                     /gene="clpX"
                     /locus_tag="PKK03_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNX84728.1"
                     /translation="MDKCSFCGRTKKEANLLIAGLEGHICDHCIEQAHNIMVEELGGK
                     SSFNLDGIKLRKPDEIKKFLDQYVIGQDRAKKILSVAVYNHYKRLMQKADSDDVEIEK
                     SNIILVGETGTGKTLLARTIARMLHVPFTIVDATVLTEAGYVGEDIESLLTRLLQNAD
                     YDVKAAEKGIVFIDELDKIARKGDNPSITRDVSGEGVQQGLLKLLEGSVVNVPPQGGR
                     KHPEQKMIPVDTRNILFICGGAFEGIEKKIAHRLNTTVVGYTASRSRDHYDKDDLLKY
                     IAPQDLRAYGLIPEIIGRLPVLTHLSPLDRKALRAILTEPKNAITKQYVKLFAMDGIE
                     LTFTPEALDYIVDKAVEFRLGARGLRAICEAIMMDAMFELPSGDVKEFTVTTEYAAHK
                     LDRVDMKVLKAS"
     gene            complement(6941..7609)
                     /gene="clpP"
                     /locus_tag="PKK03_09775"
     CDS             complement(6941..7609)
                     /gene="clpP"
                     /locus_tag="PKK03_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNX84729.1"
                     /translation="MKDFKKYAADRFGISSMTMHRFISASNSYISPTIIEERQLNVAS
                     MDVFSRLMMDRIIFLGLPIDDYVANIIQAQLLYLDSADPGKDIQIYLNTPGGAVHAGL
                     GIYDTMQYIAADVATICTGMAASMGAVLLTAGAKGKRSALKHSRIMIHQPMGGAEGQA
                     SDIEIAVKEILKAKRELYEIIAFHTGKTVEKVEKDSDRDYWMTAVEAKEYGMIDEVLI
                     RNKS"
     gene            complement(7672..9003)
                     /gene="tig"
                     /locus_tag="PKK03_09780"
     CDS             complement(7672..9003)
                     /gene="tig"
                     /locus_tag="PKK03_09780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNX84730.1"
                     /translation="MNVTRENIDALNATVRIDIAKTDYEEKVEKKLREYRRTASIKGF
                     RPGHVPIQMIKKMYGTTVLVDEINNLVSESLSEYIKNEKLDILGDPIPKNDGHTFDPE
                     KSDEFSFTFELGLAPEFEVNLNKKQKLTRYLIAPDTKMIADYVDNYSRRYGQFITAGA
                     AEEKDLLKGIVESSDGSVKNDAATLSVEMIKDEEIKKHFLGKKAGETVEFEIRKAFPN
                     DYEIAGLLKKQKDEVKDLQGSYSIQIMEVSRFVPAENNQELWDKVYGEGTVKSATEFE
                     AKVTEEIREFFNRETEYKLRTDARDLALGKTPFELPEDFLKRWLLRVNEKTTAEDIEK
                     DFDHFRDDLRWQLIKNRVAKDNELKITDEEILEEAKAFTRAQFSQYGLHYASDEQITS
                     FASDFLKKEEEARRIAEKVLDTRVLGLLIDAVKVDEKTVTPEEFNKLFGNK"
     gene            complement(9082..9166)
                     /locus_tag="PKK03_09785"
     tRNA            complement(9082..9166)
                     /locus_tag="PKK03_09785"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9130..9132),aa:Leu,seq:tag)
     gene            complement(9223..9948)
                     /gene="lptB"
                     /locus_tag="PKK03_09790"
     CDS             complement(9223..9948)
                     /gene="lptB"
                     /locus_tag="PKK03_09790"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNX84731.1"
                     /translation="MKLYTTNLVKRYKTRTVVNEVSVEVEQGEIVGLLGPNGAGKTTT
                     FYMIVGLIRPNGGQIWLDNTQITDFPMYRRARLGIGYLAQEASVFRKLSVEDNLMAVM
                     ELSDMTRQERKERQESLLEEFGLARIRKSLGIQLSGGERRRTEIARALALKPNFILLD
                     EPFAGVDPIAVEDIQMIVSHLKDKNIGILITDHNVHETLSITDRAYLLFEGKILKQGT
                     ASQLADDEQVRNVYLGKNFELRR"
BASE COUNT         2340 a         2595 c         2557 g         2505 t
ORIGIN      
        1 acacctttct tgtgtgccat atcgttttct ttttactgtt tatcggttat tccttcgccg
       61 gtttctttgt gctttttgcc ggagccttct ttacggtttc cttcttctcg gccgtttcct
      121 taacggcagc ggcttttttt gccggagcct tcttcacctc tgccttcacc tcttctgttg
      181 cgggcgcttc ggctgctttc ttttctgcct tcggtgcggc tttcttcctg ggctcaccgg
      241 ccgggccgat gctcagtatc tccacctgtg tgaaatcctg acggtgaccg gtcctcttct
      301 gataaccctt ccttctcttc ttcttgaaga tgatgacttt gtctcctctt gcatggccga
      361 gaaccttacc ctcaataaca gtattgggaa caaggggttc acccacgatg actgcaccgt
      421 cattgtctgt aagaagaact ttttcaaact ctacactgtc gccttcagct gcatcaagac
      481 ggtgaacata tatcttctgt ccctcttcta ccttgaactg ctgaccggca atttcaacga
      541 ttgcgtacat tttcaatctc tcttatatta ctccgtgagg cggacatgct cctctgtgca
      601 tgacacttct gcaggccccg ccggatctta gtttttcgga ctgcaaagat ataaaaatta
      661 acttatctgc aaaccggtga cattaattta tcaccaatat gatgtggata aaaaatcggg
      721 aaggggagga gataaagatt catattattt atatttgtca tatcggcctg tcaggcagaa
      781 aatctggtaa actatgcaga ataagctcat tcgtctgaag gttataggca tttcatacag
      841 ccagacgcag tcaggcgcct atgcccttat actggcggag gaaaacgggg aacggcggat
      901 acctataatt atcggggggt ttgaggctca ggccatagtc atcaaacttg aaaacctgga
      961 gcccccaaga ccgctgacac atgacctctt ccgcagcttt gccgaagcat gcggcgtcac
     1021 ggtggaacag gtgctcatca accggctccg cgaagggata ttctattctg agatcgtatg
     1081 cactgacggt gaaaggcgct ttcacattga ctcacgcacc tctgacgctg tggcactggc
     1141 actgaggttt gaatgcccga tattcattga acctcagatt ctgaatgagg ccggcattgt
     1201 catgagtgcc accaccgagg aagaacagaa gaaacaggcg gaaccgccga aggagaaaaa
     1261 acagcccgtg agcagcgaat acagcgaatt ctcagtggat gagcttaagg atatgctctc
     1321 cgatgcaatc ggccgggagg attatgagca ggcagcagcc atccgcgacg agatggcaag
     1381 aaggaaagga caaggataat taatcaggga gaaatgaaga ttttaaggat tttgattgca
     1441 gcagggctgc tgataccggt gcccctgctg tcaaaggagt acaaggttaa atcacccgac
     1501 gggaagatca cactctcggc ggagaccacc acggggatta acctcaccgt cagttatgaa
     1561 aataaagaga taatcaccgg gctgtcgccc gaactgcaac tggaaggggt gaccattccc
     1621 ggacaacgcc cggccatagc aaaggctgtc acctcatcag tgaacaatat tctggttcct
     1681 gtggtgcccc ataaaaacag ccgggtgcct gacatcttca acgcacttga acttcgcttc
     1741 aggggcggat catcaatcga gttacgcgtt tatgatgacg gtgtggcata cagattcaaa
     1801 tcggcaatga agggaaccgt caaagtaagg gatgaacggt tcgccttttc gcttcccggc
     1861 ggagccggag ccttttggcc gctggaaaag ggcttcatgt cacataatga atgcaccttc
     1921 ttccccgcat ccatggatac catcggcccg aaacacctgg ccagcctgcc ggcgctcttt
     1981 acctccggcc cgataaacat cctcctcagc gaggctgaca tacatgacta cccgggcctg
     2041 tggataaacg gagacggccg cgggggcgtg aagggggtgc ttcccgcata ccctgctgca
     2101 gtgaaagcgc acaatgaccg caacgtttat gtgaccgggc gcgaagacta ccttgcagtg
     2161 acatcgggca cacgcactta cccgtggcgc ctgttcatca tcacccctga tgacgccggc
     2221 ctggttgaga gcgagatggt ctacaggctg ggagctcccc cggcatcggg agctgacttc
     2281 agctgggtca aacccggaaa ggtggcatgg gactggtaca acgcaaacaa tatttacggg
     2341 gttgatttca gggccggact caacaatgat acatacaggt actatataga ttttgcatcg
     2401 gagaacggta ttgaatacgt catcctcgac gagggatggt acaaactggg agatgttctg
     2461 gagcaggccc cgggatttaa cgttggtgag ctgtgcgaat atgccgcatc taaaaacgtc
     2521 ggaatcattc tgtgggttgt atggaaaacc tttcatgaca ggattgatga agccctggca
     2581 caatttgaga agtggggagt gaaggggatc aaggtcgatt tcatgcagcg cgacgaccag
     2641 tggatggtca actattatta tgaggtagct gagaaagctg cccggcataa gatgctggtt
     2701 gatttccacg ggtcttacaa gcctgacggg atggaacgca catggcccaa tgtaatcacc
     2761 cgcgagggag tcaagggact ggagaacgac aagtggagcg ctgattgcaa cccggaacat
     2821 gacctgaccc tgccgtttac ccgcatggtc gccggcccga tggactatac cccgggagct
     2881 atggtcaaca tgcagcagcg cgacttcaag ccgatatact acaggccggc cagccagggc
     2941 accagggtgc accagatggc tatgtacgtg gtcttcgaga gtcccctgca gatgctcgct
     3001 gacagcccct caaactataa gcgcaaccag gagtgcacct ccttcattgc cggtgtaccg
     3061 gtcacctggg atgaaaccag ggtggttgag gccaggaagg gtgacaacat tgtcatagca
     3121 cgcaggcacg gcactgtctg gtacctggcc gcaatgaatg actggaaacc gttcgcgacg
     3181 gaggttgacc tttcgttcct gccttccggc gaatacagca tggagatatt ttctgatgga
     3241 attaacgctg acaggcacgg agaagattac aaacatgtca cacgcagggc tgctgccgga
     3301 gaaaagatca ctctcaccct ggctcccggt ggaggatggg tggcaaagat cactccttca
     3361 gtcagataaa gagggcggca ttctatgaaa caataccttg acctgttgag ccatgtcatg
     3421 gaacatggca ttgagaagag agaccggaca ggcaccggca cggtaagcac cttcggctac
     3481 cagatgcgct ttgatctcgc cgaacggttt ccgctgatga ccaccaagaa gctgcacctg
     3541 aaatcaatca tccatgaact gttatggttc ctcagcggaa gcacaaacgt gcgttacctt
     3601 caggaaaacg gggtcagaat atggaatgag tgggctgacg ctgacggtaa tcttggcccc
     3661 atctacggct atcagtggcg atcatggcct gtaactgacg gcagaaccat tgaccaggta
     3721 aagacagtgg tcacatcact gaagaacaat cccgactcac gaagacatat catctcagcc
     3781 tggaatgtcg gggagataga aaaaatggcc cttccgccct gccatattct gttccagttc
     3841 tatgtggccg gcgacagact ctcatgccag ctctaccagc gcagcgcaga catcttcctt
     3901 ggcgtaccct ttaacatagc ttcctattcg ctgctcacca tgatgatggc gcaggtgacc
     3961 ggctacaggc cgggtgagtt tattcacacc ctgggcgatg cgcatatata cctcaatcat
     4021 attgaccagg taaagctgca gctcacccgt gatccgcgca gacttccggt tatgagcctg
     4081 aatcctgatg taagggatat cctcggtttc acttacgctg acttctccct ttcggaatat
     4141 gatccgcacc ccgccataaa aggcgatata tctgtatgaa atgatcacta ttatagtagc
     4201 aactgaccgc aacaacggca tcggatacaa caacagcctg ctggcacaca tcccgggtga
     4261 cctccggaga tttaaggata ttaccatggg ccattgcctt atcatgggca agaagacgtg
     4321 ggaatcactc ccgaacaaac cgctcacagg caggaagaat atagtcctta ccgacaatga
     4381 gctcgactgc ttcaactgcg ctgagacagc acggtcaatt gatgaggcgc tgagcctctg
     4441 tgatcccggc agggaaatat tcatcatggg aggcggatct gtctacagac agttcctgcc
     4501 actggctgac agactgatgg tgactcatat ccactgggag taccaggctg acaccttctt
     4561 cccggagata agtccggatg aatggtatgt ttcggaacag gaggatttcc tttcggaaga
     4621 cccttctgag atcagctttt cctataccac ctacctcagg cgcaggtaac ttttctttgg
     4681 ctcatccgtt aaaccgtggg tgttttgtac tgtcaaagca atacaaacac caattgaaca
     4741 tttaacagat tctgcttatg aaaatcaaga cactaatcct gttcacagcc cttacggggc
     4801 tgataatgac atcttgcagc aaatcggacc cgattgacac aaaatccact gacgctgctg
     4861 atgacgtggc actgagcgag gctctctatg atgatgcgtt tgcatcactg gagatagcca
     4921 ctgtatttgc cgaggcgggg aaaaaatcag cctatgtcct tgacagttgt ccgctgatta
     4981 caatcaactc cccgggcgac gagttcttcc cgagaaatat tgttatcgac tacggcaccg
     5041 ggtgtaccgg attgtttgac attgagcgaa gcggaaagat cctgatcacc ctttcgggcc
     5101 cgaggggcac caccgggacg gagcgggttc ttacatttga caattattat gtaaacggag
     5161 caaaggtgga ggggaccttt tcaattgaaa acctcggtcc aaacaacaac cagaatgttg
     5221 ttttcggggt cagcctcgct gggggtaaga taacattccc tgacagcaag gtcatcaccc
     5281 gtgagttcac ccgccagcga gagtatattg ccggatacgg tacatggaat ccatgggacg
     5341 acaggtgcct gataaccggt atgtcagcgg gaacgaacct cgacggaaag acatttgctc
     5401 tgacaataac cagtccgctt gagtggactg cagcctgcag gttccttgtc accggaacca
     5461 tcggatttga cgtggagggc atagagcctt tcgtgctcga ttatggcact ggtgaatgtg
     5521 atgcactggc caccctctcg agaggagatg agaccaggga gataacgttg cgttactggc
     5581 atccgaaata ccagggcaat caatgatctg cactgacccg cgcagtttcg cggaactgtg
     5641 aaacggtcag gttaaaaaga aatcccggcg agccatctgg cggaccggga ttcttttttt
     5701 tatttttcgc ggattacgat gccttgagca ccttcatgtc caccctgtcg agcttatggg
     5761 ctgcatattc ggtggtaact gtaaactcct tcacatcgcc ggatggcagc tcgaacatgg
     5821 cgtccatcat gatggcttcg cagatggcac gcaggcctct tgcacccagc ctgaactcca
     5881 cggccttgtc gacaatgtag tcaagggcct cgggggtaaa ggtgagctcg ataccgtcca
     5941 tggcaaacag cttgacatac tgttttgtga tagcattctt aggctcggtg agaatggctc
     6001 tcagcgcttt acggtcaagg ggagagaggt gggtcagcac cggcagacgg ccgatgatct
     6061 ccggtatgag accataagca cgcagatcct ggggtgcgat gtacttcagc aggtcatcct
     6121 tgtcataatg gtcacgtgag cggctggcag tgtatcccac cacggtggtg ttgagcctgt
     6181 gggctatctt cttctctatc ccctcgaagg cgcctccgca gatgaagagg atattcctgg
     6241 tatcaaccgg tatcatcttc tgctcggggt gtttcctgcc accctgcggg ggcacgttca
     6301 caaccgatcc ttcgaggagc ttgagcagac cctgctgaac accctcacct gatacgtcac
     6361 gggtgattga gggattatcg cccttgcggg ctatcttgtc aagctcgtca atgaacacta
     6421 ttcctttctc tgcggccttg acatcatagt cggcattctg aagcaggcgg gtaagcaggc
     6481 tctcgatgtc ctcaccaaca taacctgcct ctgtcaggac ggtggcatca acgattgtga
     6541 aggggacatg aagcatgcgg gcgattgtgc gcgccagcag tgtcttgccg gtaccggtct
     6601 caccaaccag gatgatgttc gatttctcaa tctccacatc atcactgtca gccttctgca
     6661 tcagcctctt gtagtgattg tagacagcca ccgagaggat cttctttgcc ctgtcctgcc
     6721 cgatgacata ctgatcgaga aacttcttta tctcatccgg cttgcggagt ttgatgccgt
     6781 caaggttaaa gcttgatttg cctcccagct cctctaccat aatattatga gcctgctcta
     6841 tgcaatgatc gcatatatgg ccctcaagac cggcgatgag aaggttagcc tctttcttgg
     6901 tacgtccgca gaaggagcat ttatccatta gtggaaaaaa ttagcttttg ttcctgatca
     6961 gtacctcgtc aatcataccg tattccttcg cttctacagc agtcatccag tagtcgcggt
     7021 ctgaatcctt ttcgaccttt tcaacagttt tgccggtatg aaacgcgatt atctcataga
     7081 gctcccgttt ggccttcagt atctccttga cggctatctc gatgtctgat gcctgtccct
     7141 ctgcgccacc catcggctga tggatcataa tgcgggagtg tttgagtgcc gagcgtttgc
     7201 ccttcgcacc ggccgtaagc agtactgcgc ccatcgaggc ggccatgccg gtgcatatgg
     7261 tggctacgtc ggctgcgatg tactgcatcg tgtcatagat gcccagccct gcatgtaccg
     7321 caccgccggg tgtgttgagg tagatctgta tgtccttgcc cggatcagcc gagtcaagat
     7381 aaagcagctg agcctgtatg atattggcca catagtcgtc aattggcagg cccaggaaga
     7441 tgatacggtc catcataaga cgcgagaaga cgtccataga tgccacgttc agctggcgct
     7501 cctcaataat cgtcggggag atgtagctgt tgctggccga gatgaaacgg tgcatcgtca
     7561 tgctgctgat gccgaaccgg tcagcggcgt attttttgaa atccttgatg tctgacatgg
     7621 tatgaaactt tgattgaatg ccaaatatac gaaatattag gctttaaagt ttcacttatt
     7681 gccgaagagc ttgttgaatt cctcgggagt gaccgtcttc tcatctacct tgacggcgtc
     7741 aatcagaagg ccgagcaccc ttgtgtcaag cactttttct gcaatgcggc gggcctcctc
     7801 ctccttcttc aggaaatcac tggcaaatga tgtaatctgc tcgtcagaag cgtaatgcag
     7861 gccatactgg gagaactggg cacgggtaaa agcctttgcc tcttcgagga tctcctcatc
     7921 ggtgatcttc agctcattgt cttttgcaac cctgttcttt atcagctgcc agcggaggtc
     7981 atcacggaag tgatcgaaat ccttttctat atcctctgcg gtagtttttt cattgactct
     8041 tagcagccat ctcttgagga aatcctcggg cagctcgaac ggggttttcc ccagagccag
     8101 gtcacgggcg tctgttcgta acttgtattc agtctcacgg ttgaagaact ccctgatctc
     8161 ttcggttacc tttgcctcga actcagttgc tgacttcacg gttccctcac cgtaaacctt
     8221 gtcccacaac tcctggttgt tttcagccgg aacaaagcgg gatacttcca ttatctggat
     8281 cgagtagctc ccctggaggt ctttgacctc atccttctgt ttcttgagca ggcctgcaat
     8341 ctcataatca ttcgggaatg ccttacggat ttcgaattcc acggtctctc cggctttctt
     8401 tccaaggaaa tgcttcttta tctcctcatc ctttatcatc tccactgaca gggtggcagc
     8461 atcatttttc accgatccgt cagatgactc cacgataccc ttaagcaggt ccttctcctc
     8521 tgctgcaccg gccgtgatga actggccgta cctgcgggag tagttgtcaa cgtaatcggc
     8581 aatcatcttt gtgtcaggtg caataaggta gcgggtgagc ttctgctttt tgttaagatt
     8641 cacctcaaat tcgggcgcca gcccaagctc gaaggtgaaa ctgaattcat ccgacttctc
     8701 cgggtcaaat gtgtgaccgt catttttcgg aatcgggtca cccaggatgt caagcttctc
     8761 atttttaatg tattcagaaa gactttccga tacaaggttg ttgatctcgt cgacgaggac
     8821 ggtggttcca tacatcttct taatcatctg gataggaacg tgtccggggc ggaatccctt
     8881 tatactggct gttctccggt attccctgag tttcttttca accttctctt cataatcggt
     8941 tttggcaata tcaatcctga ccgttgcatt cagtgcgtca atgttctctc tggtgacatt
     9001 catcttgtaa aagtttacta tcgatttaaa aaccgccata acctgcgcca tgaggcgaag
     9061 tgtcagagcg gttcaggtgt ctggtgcgga tgaagggact cgaaccccca cgccctgagg
     9121 cacaagatcc taagtcttgc tcggctacca attacgacac atccgcaaaa agtggtgcaa
     9181 aattagtaaa aaatacgaag ttgcaaaagg gtccggatta atttacctcc tgagttcgaa
     9241 gttcttaccg aggtaaacgt ttcttacctg ttcatcatca gcaagctgcg aagcagtgcc
     9301 ctgcttgagt atcttcccct cgaacaggag gtaggccctg tcagtgatgg acagggtttc
     9361 gtggacgtta tggtcggtta tcaggatgcc tatgttcttg tctttcaggt gagagacgat
     9421 catctggatg tcctctactg caatagggtc aacgccggcg aagggttcgt cgaggaggat
     9481 gaagttaggt ttgagggcca gggcgcgggc gatctccgta cggcggcgtt cacctcccga
     9541 aagttgaata ccgaggctct tgcgtatgcg tgccaggccg aactcctcaa ggagtgactc
     9601 ctgacgctcc ttcctctcct gcctggtcat gtcagagagc tccatgacag ccataaggtt
     9661 gtcttcgacg ctcagtttgc ggaagacgga ggcttcctgt gccaggtagc cgataccgag
     9721 gcgtgccctg cgatacatcg gaaagtcggt tatctgggtg ttgtcaagcc agatctggcc
     9781 tccgttcggc cgtatcagtc ccacgatcat gtagaacgtg gtggttttgc cggcgccgtt
     9841 gggaccgaga agacccacta tttctccctg ctcaacctca acggagacct cgtttaccac
     9901 tgtccgtgtc ttgtaccgtt tgacaagatt tgttgtatag agcttcatag tttcactgta
     9961 agttggctgc aggttcatcc gttcctcccg gctgcct
//
LOCUS       DAOPXU010000103         9946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_7962_length_9946_cov_11.558892, whole genome shotgun sequence.
ACCESSION   DAOPXU010000103 DAOPXU010000000
VERSION     DAOPXU010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9946
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7962_length_9946_cov_11.558892"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PKK03_09795"
     CDS             <1..839
                     /locus_tag="PKK03_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNX84732.1"
                     /translation="VRKIEIRSRGLTRQIFAGEYHSAFRGRGMAFSEVREYQFGDDIR
                     NIDWNVTARFGHPYVKVFEEERELTVMLLVDVSRSGDFGTVASTKREMMTEIAAVLAY
                     SVIANNDKVGLMLFSDRVEKYIPPKKGRGHMLRIIREMLGYEPSGSATSLSEPLRYLT
                     NIIRKRCTAFILSDFLAPPFTDALRIASGKHDVVALRISDRREKEMPDVGFIKVLNPE
                     TGREQWIDTSSSAVRKDYAAQWKRHDEAVASAFVSCGVDMASISTGEDYIKPLISLFK
                     HR"
     gene            844..1872
                     /locus_tag="PKK03_09800"
     CDS             844..1872
                     /locus_tag="PKK03_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84733.1"
                     /translation="MRKAFISIVVFLTSVLLLSAQQIRVTSKPDTSVMLIGDQIGFTV
                     TAEMPSGVSADMSGAADTLAGKIIILRRDPRDTTVSANGSVTITDRYLITAFDTGSYT
                     IPPFYAGVASGDSLLRFFSDYSFLDVIRPDVTPPDTTDVIFDIVGPRSAPVTFREILP
                     WIIITLIVAVMVWLLARFLPRNPLRRFVRPPAPPEPAHVIALRDLKTLRSEEIWQKGD
                     IKEYYSRLSDILRRYIDNRYGVSSPELTTDETVRMLQKASVTTSGQMAIVKEVLSLSD
                     MVKFAKYLPDSALHESSMDSAVRFVEETRLPDAAPADTKDKTENTSGEGSDGAVAGKQ
                     KEKGGRHA"
     gene            1874..2857
                     /locus_tag="PKK03_09805"
     CDS             1874..2857
                     /locus_tag="PKK03_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNX84734.1"
                     /translation="MVFENSWFLWFLLIIPLLVAHYIWRQGKANAPVKLSTAGFFAEG
                     RKTFRHYLRHLLFALRVAAIALVIVVIARPQNVDRWQSSTTEGIDIILALDVSGSMLA
                     RDFNPDRLEASKNVATEFISGRPYDRMGLTVFSGESFTQCPLTTDHAVLINLLREVKS
                     GIIEDGTAIGVGLATAINRIKDSDAISKVIILLTDGVNNRGSIDPMTAAEIAKTFGIR
                     VYTIGVGSMGYADYPVQTPFGMRYQKMEVEIDEALLQRIAEMTGGQYFRAVDNTSLQK
                     VYGEIDKLEKSKIETREHSRRDEVFMSWALAALALLGLELLLRYLLMKNMS"
     gene            2865..3920
                     /locus_tag="PKK03_09810"
     CDS             2865..3920
                     /locus_tag="PKK03_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNX84735.1"
                     /translation="METFRFAQPDMLYLLLLVPVLIILWIIGNRKRRLARERFGDAPM
                     LEQLSPDYSPARMAVKFIIRLLAFVMAVLTVARPQFGSRLEEVKREGVEVVIALDVSN
                     SMLAADITPSRLERAKQAITQLVDQLKEDRIGLILFAGDAYTQIPVTNDYLSAKMFLS
                     TAGPDAVSKQGTAIGAAIDLGIRSFTPESDKSRALIIITDGENHEDDAVEKAREAAEK
                     GIVVHTIGIGSPDGSPIPVVTGGRTDYLKDSQGNTVISKLDEKGLQEIASVTGGRYVR
                     ASNTSIGLTEIYNDIGRMKKSEINAMMYTEYNEQFLIPAALLFILLVTDLLLADRKNN
                     LLRRIGIIGKEPKQDRI"
     gene            3917..4651
                     /locus_tag="PKK03_09815"
     CDS             3917..4651
                     /locus_tag="PKK03_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX84736.1"
                     /translation="MRGNGTAYRLLIAVICTAVISPSLHGQEEREYIRKGNRLYKKSE
                     FAGSEGMYRRAQEDGKPSGDAVFNLGDALYRQNRYGEAAEEFSRAVQAGETDSLRQAD
                     GLYNLGNSLLKEQKFSESINAYINSLKLNPDNIQAKYNLAYAQDQLKKQEEQQQQQDQ
                     QDQDKNRQDQQNKDQEDKKDQEQKDDQQQQQQDQQNNQQQQQSSMSREDAKRLLDALA
                     ANEKQTQEKVQRDKAAGARVRVIKNW"
     gene            4662..6482
                     /locus_tag="PKK03_09820"
     CDS             4662..6482
                     /locus_tag="PKK03_09820"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNX84737.1"
                     /translation="MKRITTALVFLLACLAATAQEVTLTVEAPESAVAGERFRIIYTI
                     NSTDGQFTPPKFDPSFSVQGPQQSTSRNVQWINGDMKAVSSTTLIYYVTVGAPGTYTI
                     PAAQFEAKKVTVSSQERQIVISAGGSPQQAAGQQQGQGQVKAAGDAPASGSEVSMKIL
                     LGDREVYAGEPVTGMLKLYTRINLSGIQELKFPDFKGFLKEDIETPPLRSLETEVIDG
                     IQYGTGVIQRFVLYPQVSGEIRIEPAEMTVLVQERTGARDPFFDDPFFDSFFSSVSTV
                     PRKITTQPLTVRVKSLPEPRPADFHGAVGSFTMESSISGTDAKLNDAVTMTVTLKGSG
                     NLNLSGEPVISFPQGIEKYDPKVTVKSSGTSSGTKVFEYLLIPRNTGTFEIPPISYSV
                     FDPKEEKYVTLRAGGFTVNVTGTPGQEESAAPVYIPGEDVKYLGQDIRFIRNGKTKLA
                     AISSPLVSTLYYWLWFALALFVAVLVLVVRKQQIRRNADTAGVRNRRAAKNARKRLAR
                     ATALLKSGKKEEVNAEVARALWGYLGDKLRIAQSDITRDKCYIALRDRKADEEMITEL
                     DRILTATEYSQYAPVSEGESPAALCERAATLIGKLDDVLD"
     gene            6495..7253
                     /locus_tag="PKK03_09825"
     CDS             6495..7253
                     /locus_tag="PKK03_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84738.1"
                     /translation="MRRILLIQIIFMAGILSLEAQSPEERYSQAGGLYGSGDYSGAAA
                     IYRKLWEEGFRSEDLLYNAGNAFFKSGDNASAILFYERAKLLAPADEDIDYNLQIARS
                     RITDRFESVPQLFFVRWFDFLALLTSTNTWAVSALVMFLVALVLALLFLTRARARGRL
                     LSFWLAITALLLSFLIISLALRNNNLVNHNSKAVITCSIVTGRSAPGETGNELFVLHS
                     GTTVTVEQELGAFLEVRLPDGNKGWIRSDCMEKI"
     gene            7304..7723
                     /locus_tag="PKK03_09830"
     CDS             7304..7723
                     /locus_tag="PKK03_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84739.1"
                     /translation="MIGKLPVVLLAAVLLLTSCKKRNITDEGPTDVRIHNQTGQIIND
                     VNVTVTDDPAYAVRTHNFGTVAAGAYSDYFRFDIAHTEADITVKIGNVTYSTPSSQFD
                     YLTYIGPDRITYRITITDPVNRVLDIETVIEEPIDNL"
     gene            7732..9756
                     /locus_tag="PKK03_09835"
     CDS             7732..9756
                     /locus_tag="PKK03_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNX84740.1"
                     /translation="MKELITVCPRNCYSTCTFRVFTEDGRVVRILPFEGNLATPEGPC
                     IKGLSYLEREYSPERIIYPLRRQPDGSFARIGTDEALTIIAGRLVEARKNHGPHSVLF
                     YKGSGYSGLSNEIAGSFWRLFGGATTTYGNLCWPAGLEAVRLTLGEVKHNLPWDLANA
                     SMIILWGKNSAETNVQELIHIEKARQNGAKVVVIDPRRTLTADKADMLIRPRPGTDAA
                     LALAMARVIIDRGLTDKEFISRHVSGFGQFAASLQITPDKAQDITGIPAQTIIDLAVA
                     AAGAGRLTLVAGFGLQRYTNGGQTIRSILSIPVITGMIGRPGCGFNFANLQSYIFDEV
                     KEPLSYYPDPGTDKPFRRAISMARFGPDFFALGEPAIKFAWIERGNPVIQLPASAEVR
                     KALESVPFKVVVEQFMTDTAAMADIILPAKGVFEQADVVGSYWNPYVQYKPQVTDPPG
                     EVLPESEIYWRLAQFMGLNTGEAGIPAPGQYDSWLMERISHTPGFVREELLQKPVIPD
                     QNEHPAYGNLKFDTPSGKIELLSDSAAKLWGVNPLPTFEPLNDLGEERLPLRLMTPNI
                     ASRIHSQFGNLEVVRSVVEEPAWEISVADARSRGLRSGDRIRVFNSRGEVTGKVRVTA
                     RVRSGSLVFPNGIWLGEGGGVNRLIASSETDMGHGAAFHNTRVDIEKLVP"
     gene            9753..>9946
                     /locus_tag="PKK03_09840"
     CDS             9753..>9946
                     /locus_tag="PKK03_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84741.1"
                     /translation="MRKGFTFDAALCINCKACSAACILENGLRPGTRTLFTWNEHALP
                     LAGVINLSMACNHCAEPACAS"
BASE COUNT         2486 a         2519 c         2756 g         2185 t
ORIGIN      
        1 aggtaaggaa gattgagatc aggtcgcgcg ggctgacccg ccagatcttt gcaggtgagt
       61 atcacagtgc tttccgtggc aggggaatgg ccttcagcga ggtacgcgaa taccagtttg
      121 gtgatgacat ccgcaacata gactggaacg tcaccgcccg gttcggtcat ccttatgtca
      181 aggttttcga ggaggagaga gagctgacag tcatgctgct ggttgatgtg agcaggtcgg
      241 gagatttcgg aacggtggct tccaccaaga gggagatgat gacggagata gctgccgtcc
      301 ttgcatattc ggttattgcc aacaatgaca aggtagggct gatgctcttc tctgaccgtg
      361 tggagaaata cattcccccg aagaagggca gaggccatat gctcaggatc atccgcgaga
      421 tgctcggtta tgagccttca ggatcagcca catcgctgtc cgagccgtta aggtacctga
      481 ccaatattat caggaagagg tgtaccgcct ttattctttc tgatttcctt gctcccccct
      541 ttacagatgc ccttcgcatt gcatcgggca agcatgacgt ggttgccctc cgcatcagtg
      601 acaggcgcga gaaggagatg cctgacgttg gtttcataaa ggttttgaat ccggagacgg
      661 gcagggaaca atggatagac acctcctcat cggctgtcag gaaggattat gccgctcagt
      721 ggaaaaggca tgatgaggct gttgcctcgg cctttgtcag ctgcggagtt gatatggcat
      781 caatcagcac cggcgaagac tatatcaaac cacttatctc actgttcaag cacaggtagg
      841 aatatgagaa aggctttcat ttcaatagtg gttttcctta catcggtact actcctgtca
      901 gcccagcaga taagggttac ctcgaagcct gacacctcgg tcatgctgat aggtgaccag
      961 attggcttca ccgtcacggc agagatgcct tcaggtgtct ctgctgatat gagcggtgcc
     1021 gccgacaccc tggccggaaa gatcatcatt ctgcgcagag acccgcgtga cacaactgtt
     1081 tcagcgaacg ggtcggtaac aatcactgac aggtatctca tcacggcatt tgataccggg
     1141 tcatatacca ttccgccatt ctatgcgggg gtggcttccg gtgacagcct gctgagattc
     1201 ttttcggatt actcatttct ggacgtaatt cgtcccgacg tcaccccgcc tgacaccact
     1261 gatgttattt ttgacatagt cggccctcgc agtgcacctg taaccttccg ggaaatcctg
     1321 ccatggataa taatcaccct gattgttgca gtgatggtat ggctgctcgc acgcttcctg
     1381 ccaaggaacc ccctccggcg ttttgtcaga ccaccggccc cgcctgaacc ggcacatgtc
     1441 attgcgctgc gtgacctgaa gactctcagg agtgaggaaa tatggcagaa gggtgatatc
     1501 aaggaatatt actccaggct ttcagacata ctcaggagat atatcgacaa ccgttacggt
     1561 gtcagctctc ccgaactgac gacggacgag accgtgcgga tgctgcagaa ggcatctgtg
     1621 accacttcgg gacagatggc tattgtcaag gaagtgcttt ctctctcaga catggtcaag
     1681 tttgccaaat acctgcccga cagtgctttg catgaatcat ccatggatag tgccgtgagg
     1741 tttgtcgaag agaccaggct tccggatgca gctccggctg ataccaagga taaaactgaa
     1801 aatacttctg gcgagggcag cgatggagca gtcgcaggaa aacagaagga gaaaggaggg
     1861 cgccatgcat aacatcgtct tcgagaacag ttggttcctc tggttccttc tcatcatacc
     1921 cctcttggtg gcacactaca tatggcgcca gggaaaagcc aatgccccgg tgaagctttc
     1981 cactgccggg ttctttgccg aagggcgcaa gaccttccgg cactacctga ggcacctgct
     2041 ctttgcccta agggtggctg ccatagcgct ggtaatagtg gtaattgcca ggcctcagaa
     2101 tgttgacagg tggcagagct ccaccactga ggggattgat attatccttg ctctcgacgt
     2161 ttcagggtcg atgcttgcgc gcgacttcaa tcccgacagg ctggaagcat ccaagaacgt
     2221 cgcaacggaa ttcataagcg gaagaccgta tgaccgcatg ggactcacgg tattcagcgg
     2281 cgagagcttt acccaatgtc ccctcacgac agatcatgcc gtgctcatca acctgctcag
     2341 ggaagtgaag agcggaatca ttgaggatgg cacggccatt ggcgtgggac tggccactgc
     2401 aatcaacagg ataaaggatt ccgatgccat cagcaaagtc ataatcctgc tcactgacgg
     2461 cgtcaataac cgtggatcca ttgacccgat gacggctgca gagattgcaa agacctttgg
     2521 catcagggtg tatacaatcg gtgtgggcag catgggatat gccgactacc cggtacagac
     2581 acctttcggc atgagatacc agaagatgga ggtggagatt gacgaggctc ttcttcagcg
     2641 gatagctgag atgaccggag ggcagtattt cagggctgtg gacaacacca gcctgcagaa
     2701 ggtatacggt gagattgaca agcttgagaa atcaaagata gagaccagag agcacagccg
     2761 gcgtgacgag gtgttcatgt catgggccct cgcggccctg gcgctgctcg gcctcgagct
     2821 gttgctcagg tatctcctca tgaaaaacat gtcatagcgc gattatggaa acattcagat
     2881 ttgcacagcc tgacatgctt tacctgcttc tcctggtacc ggttctgatc attttatgga
     2941 tcatcggcaa caggaaaagg agactggcca gggaacgttt cggcgatgcg ccgatgcttg
     3001 aacagctctc gcctgactat tcccctgcaa gaatggcggt taaattcatc atcaggcttc
     3061 ttgccttcgt catggccgtg ctcaccgttg caagaccgca gttcggctcc cggctggaag
     3121 aggttaagcg tgagggagtg gaagtggtga tagcccttga cgtaagtaac tcgatgcttg
     3181 ctgccgatat aaccccctcg aggctcgaaa gggccaaaca ggctataacc cagctcgttg
     3241 atcagctgaa ggaagaccgg atcgggctca ttctctttgc cggcgatgca tatacgcaga
     3301 tccctgttac caatgactat ctctcggcca agatgtttct gtcaaccgca gggcctgatg
     3361 cggtatcaaa gcagggaacc gcaatcggcg ctgccattga cctggggatc agatccttca
     3421 cccccgagag cgacaagagc agggcactga taataatcac ggatggtgaa aaccatgagg
     3481 atgacgcggt tgaaaaggca cgggaagcgg ctgagaaggg tatagtggta catacaatag
     3541 gcataggatc gcctgacgga tctcccatac cggtggtgac cggcgggaga accgattatc
     3601 ttaaagacag tcagggaaat acagttattt cgaagcttga cgagaaaggt ttgcaggaga
     3661 ttgcatcggt aacgggcgga aggtatgtca gggccagcaa cacaagcatc ggactgactg
     3721 agatttataa tgacattggc aggatgaaga agagtgagat taacgccatg atgtacaccg
     3781 agtacaatga gcagttcctt atacctgcgg cacttctctt cattctgctt gtgactgacc
     3841 tgctgctggc tgacaggaag aacaatcttc tcaggcgtat tgggatcatc ggaaaggaac
     3901 cgaaacagga caggatatga gaggtaatgg aacagcttac agattgctga ttgccgtcat
     3961 ctgcacggca gtaatctctc cttccctcca cggacaggag gagagggaat atatcaggaa
     4021 gggaaacagg ctttacaaga agagtgagtt tgccggcagc gaagggatgt accgtcgtgc
     4081 acaggaagac ggcaagcctt caggcgatgc ggtcttcaac ctcggtgacg ccctctacag
     4141 gcagaacagg tatggcgaag ctgcggagga attcagccgt gcggtacagg ccggcgaaac
     4201 cgattcactc aggcaggctg acggcttata caaccttggc aactcattgc tgaaggagca
     4261 gaaattcagc gaaagcatca atgcttatat caactcgctg aaactcaatc ctgacaatat
     4321 ccaggcaaag tacaacctcg catatgccca ggaccagctg aagaaacagg aggaacagca
     4381 acagcagcag gatcagcagg atcaggacaa gaacaggcag gaccagcaga acaaggatca
     4441 ggaggataag aaggaccagg agcagaagga tgatcagcag caacagcagc aggatcagca
     4501 gaacaatcag caacaacagc agtcctcaat gagtcgtgag gatgcgaagc gccttcttga
     4561 cgcccttgct gccaatgaaa agcagaccca ggagaaggtg cagcgcgaca aggctgccgg
     4621 tgcaagggta agggtcatta aaaactggta attttgcagt gatgaaaagg ataacgacgg
     4681 cgttggtttt tctgctggcc tgccttgcgg caacggccca ggaggtaacg cttaccgttg
     4741 aagcaccgga gagcgccgtt gccggagaaa ggttcaggat catctatacg atcaactcaa
     4801 cagacgggca attcacccct ccgaagttcg atccttcatt ctcggtccag ggtcctcagc
     4861 agtcgaccag ccgcaatgta cagtggatca acggagacat gaaggcggtt tcttccacca
     4921 ccctcattta ttatgttaca gttggtgcac caggcactta taccattccg gcggctcagt
     4981 tcgaggccaa aaaagtcact gtcagctcgc aggaaaggca gatagttata tctgccggag
     5041 gttccccgca gcaggctgca ggccagcagc agggccaggg gcaggtaaag gcggccggtg
     5101 atgctcctgc ctcaggatca gaggtctcca tgaagatact gctcggcgac cgtgaggttt
     5161 atgcaggtga gccagtcacc gggatgctga aactttatac ccgcattaac ctttcaggca
     5221 tacaggaact taagtttcct gatttcaagg gttttctgaa ggaggatatt gaaaccccgc
     5281 ctctgcgcag cctcgagacc gaggtgattg acggcataca gtacggcacc ggcgtcatcc
     5341 agcgttttgt actctatccg caggtttcgg gtgagatacg catagaaccg gccgagatga
     5401 ccgtcctggt ccaggaacgg accggtgcaa gggatccctt ctttgatgat cccttcttcg
     5461 acagtttctt ttcatcggtc tccaccgtac cacggaagat taccacacag cctctgactg
     5521 tcagggtcaa atccctgccc gaaccccggc cggcagattt ccacggcgct gtaggttcct
     5581 ttacaatgga atcgtccatt tccggtactg atgcgaagct taatgacgct gtcacgatga
     5641 ctgttaccct taaaggttcg ggaaacctca acctctccgg agaacctgta ataagctttc
     5701 cgcagggaat tgaaaagtat gatcccaaag tgaccgttaa gagttcaggc acctcctcgg
     5761 gaaccaaggt tttcgaatac ctgctcatcc cgagaaatac gggcaccttc gagataccgc
     5821 ccatatcata ttctgtcttc gacccaaagg aggaaaagta cgtcacgctg cgtgccggag
     5881 gattcactgt caacgtcacc ggcacgccgg gacaggaaga gagcgcagct ccggtctata
     5941 ttcccggtga ggatgtaaag tacctcggtc aggatatcag gttcatccgg aatggcaaaa
     6001 caaagctggc agcaatttcc tcgccgctgg tttcaacact ttattactgg ctctggtttg
     6061 cccttgccct ctttgttgct gtcctggtgc ttgtggtccg gaagcaacag atcagacgca
     6121 atgctgacac ggcaggagtt cgcaaccgcc gggcggcaaa gaatgcccgc aaaaggctgg
     6181 caagggcaac tgcacttctc aaatcaggga agaaggaaga agtaaatgcc gaggttgcca
     6241 gggctctgtg gggttatctt ggtgacaagc tccgtatagc gcagtcggat attaccaggg
     6301 acaagtgcta catagcactg agagacagga aggctgacga ggagatgatc accgaactcg
     6361 accggatact caccgctacc gagtactcgc aatatgctcc cgtttcagag ggtgaaagcc
     6421 ctgcagcact ctgtgaaaga gcagccacac ttatcgggaa acttgacgat gttcttgact
     6481 aaaggaggga gaggatgaga aggattctac tgatacagat catattcatg gccggcatcc
     6541 tgagtcttga ggcccagtca cctgaggagc gctattctca ggcaggcggt ctctacggtt
     6601 caggagacta ttcaggcgct gcagccatct accggaagct gtgggaggaa ggtttcagaa
     6661 gcgaggacct gctctacaat gcaggaaatg cgtttttcaa gtcgggagac aatgcctctg
     6721 caatcctgtt ttacgaaagg gcaaagttgc tggcacctgc agatgaggac attgactata
     6781 acctgcaaat agccaggagt cgtataacag acaggtttga atcggtgcca cagctgtttt
     6841 tcgtcaggtg gttcgatttc cttgctttac ttacttcgac aaatacctgg gcagtatcag
     6901 ccctcgtgat gttccttgtg gccctggtgc ttgccctgtt gttccttacc agggcaaggg
     6961 cgcggggacg gcttctttca ttctggctgg ccatcaccgc gctgctgttg tccttcctga
     7021 taatttcact ggccttacgg aacaataatc tcgtcaatca taacagcaaa gcggtgatta
     7081 cctgcagcat tgtgacaggc cggagcgccc cgggggagac aggcaacgag ctctttgtcc
     7141 tccattccgg caccaccgtc accgttgagc aggaactggg agcattcctc gaggtgagac
     7201 ttccagacgg gaacaagggt tggatcaggt cagactgcat ggaaaagata taatccgtta
     7261 agactatttt cttaactttc tgccggaatc aatttgtccg gacatgatag gaaaactacc
     7321 ggtggtcttg ttggcagctg tgctattgct gacatcatgc aagaaacgaa acatcactga
     7381 cgaaggtccg accgacgttc gtatccataa tcagaccggc cagattatca atgatgtcaa
     7441 tgtcacagtc actgatgatc cggcgtatgc ggtaaggaca cataattttg gcaccgtcgc
     7501 ggcaggggct tattcagatt acttcaggtt cgatattgcc cataccgagg ctgacatcac
     7561 ggtaaaaata ggcaatgtca cctattccac accctcatct caatttgatt acctgacata
     7621 tatcggtcct gaccggatca cctacaggat cacaatcaca gatccggtga acagggtgct
     7681 cgacattgaa acagtcatcg aggagcctat agacaatctt tagccccggg aatgaaggaa
     7741 ctcatcacag tctgtccgcg caactgttac agcacatgca ccttcagggt attcaccgaa
     7801 gatgggaggg tggtccgcat tctccctttt gagggtaacc tggctactcc ggaaggaccg
     7861 tgcatcaagg gactttctta cctggaaagg gaatactctc cggaacggat catttatcca
     7921 ctcaggagac agcctgatgg ctcctttgcg cgcattggta cagatgaagc cctgactatt
     7981 atagccggca gactggtgga ggcaaggaag aaccatggtc cgcattcggt tttgttctac
     8041 aagggcagcg gttactcagg gctgagcaat gaaattgcgg gcagcttctg gagactgttc
     8101 ggcggagcca ccacaaccta cggcaatctc tgctggcctg cggggctcga agccgtcagg
     8161 ctgacactcg gcgaggttaa gcataacctg ccatgggacc tggccaatgc ctccatgatc
     8221 atcctctggg gaaagaattc cgccgagacc aacgtgcagg agctgataca tattgagaag
     8281 gcacggcaga acggagcaaa agtggttgta attgatccgc gccggaccct cactgcagac
     8341 aaggctgaca tgctcatcag accacggccc ggcaccgacg ccgccctggc actggcaatg
     8401 gcacgggtga taattgaccg cggcctgaca gataaagagt tcatcagcag gcacgtttca
     8461 ggtttcggac agtttgcagc gtcacttcag ataacgcctg ataaagcaca ggatattaca
     8521 ggtataccag ctcagacaat aattgatctt gcagtggcgg cggcgggtgc cggccggctg
     8581 actcttgtgg caggtttcgg attgcaaagg tacaccaacg gagggcagac aataaggtca
     8641 attctttcca ttccggttat cacaggtatg atagggaggc ccggttgcgg tttcaacttt
     8701 gccaacctgc agagttacat atttgatgag gtaaaggaac ctttgagcta ttatcctgac
     8761 cccgggacag acaaaccttt ccgacgggcc atttcgatgg ccagattcgg cccggatttc
     8821 tttgccctgg gagagccggc cataaaattt gcctggatcg agagggggaa tcccgttatc
     8881 cagcttcctg ccagcgcgga ggtcagaaag gcccttgagt cggtaccttt caaggttgtg
     8941 gttgagcagt ttatgactga cacggctgcg atggctgaca taattctgcc ggcaaaggga
     9001 gtatttgaac aggctgacgt ggtgggttca tactggaatc cctatgttca gtacaaaccg
     9061 caggtcaccg atccgcccgg cgaggtgctt cccgagagcg agatatactg gcggctggct
     9121 cagtttatgg gtctgaatac cggtgaagcc ggcatcccgg ctccaggaca gtatgacagc
     9181 tggctcatgg agaggataag ccacaccccg ggtttcgtaa gggaagagtt attgcagaag
     9241 cctgtcatcc ccgaccagaa cgaacatccg gcatacggaa acctgaaatt tgacacacct
     9301 tcaggaaaga tagaactgtt gtctgacagc gctgcaaaat tatggggtgt taatccgttg
     9361 ccgacctttg aaccgctgaa cgacctcggg gaggagcgtc tgcccctgag gctcatgaca
     9421 ccgaatattg catcacggat ccattctcag ttcggaaacc tggaagtggt aaggagtgtg
     9481 gttgaagaac cggcctggga aatatccgtg gctgatgccc gcagcagggg tcttaggagc
     9541 ggagacagga tcagggtgtt caattcgcgc ggtgaagtta ccggcaaggt cagggttacg
     9601 gcccgtgtca gaagcggcag cctggttttt ccaaacggca tatggcttgg cgagggcggg
     9661 ggagtgaaca ggctcatcgc ttcctcggag accgatatgg ggcacggagc tgcctttcac
     9721 aatacgaggg ttgacataga aaagctggtg ccatgaggaa gggattcaca tttgacgctg
     9781 cactctgtat aaactgcaag gcctgcagtg ccgcctgcat ccttgagaac gggctccggc
     9841 ccggcacgcg cacccttttc acgtggaatg aacatgcact gcccctggcg ggagtcatca
     9901 acctctccat ggcctgcaac cactgtgccg aacccgcctg cgcctc
//
LOCUS       DAOPXU010000104         9888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_8021_length_9888_cov_9.947219, whole genome shotgun sequence.
ACCESSION   DAOPXU010000104 DAOPXU010000000
VERSION     DAOPXU010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9888
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8021_length_9888_cov_9.947219"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            784..3327
                     /locus_tag="PKK03_09845"
     CDS             784..3327
                     /locus_tag="PKK03_09845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNX84742.1"
                     /translation="MKKKTNKSPFPWNSVSVLLFTAAVLTAIIIIYSGEQKKRLLRDA
                     VTEMEAVANLKSEQVSEWKKERLNDATLIHENVTLVEHIDAMFIKDNSVREKSRLIQL
                     LTTLIESYDFGGAVLLDAGGSPGIAVPPEEAVMGEYLRGNVTKAISNPVISMSDFHTA
                     KVVKYLHLDLLIPMKMPYEGDTVLAGVLVLRIDPEKELFPMLSTWPTPGSTSECILFR
                     FEGDSVIYLNGSHLAGYDRPVTFAAGPMELSAMRPATESDEANVYNDYTGTEVIASVK
                     KVPGTDWYLIAKKETSEILARFMREITMLWMIMGIVYLALASMAWLIIKSTKARYLRE
                     RQQEELNRQAIMKHFDFVMKHGNDIILLIDSDLNIVEANERAVTTYGYPREKLIGMNV
                     SEIRTPHEIPQLSEHVRNLQETGSSYIETVHLRSDGSTFPIEISAYTVEVEGNRFFQS
                     IGRDITDRKRTEEEMRESNKKLSTIINNLRGVVFRCNNDRNWTMQYISDGIYELAGYL
                     PNEFINNKIRTYSSIIDPEDVERVWNNVHAALETGYLYTIEYRIITSSGNRKWVWERG
                     RGYYESDKLVALEGFISDITERKRIEEELIRAKEKAEQSDRLKTAFLHNISHEIRTPM
                     NAIVGFTTLLDSPDTTDDSRRQYIDIIYQSSNQLLSIITDIVDISNIETGLVKISRST
                     VNMNQLIRSLYDQYRMRVQQQGLVLNFTTHLDDRDAQVVTDETKVIQIFSNLLNNALR
                     FTKKGRIELGYVVRGEMVEFFVSDTGIGIAPEFQQKVFERFFQVEGPSSKQYSGTGLG
                     LSISKAYAELLGGSIWLISTPGEGSLFCFSIPFARPAEPVVQARTSSHK"
     gene            complement(3350..5113)
                     /gene="aspS"
                     /locus_tag="PKK03_09850"
     CDS             complement(3350..5113)
                     /gene="aspS"
                     /locus_tag="PKK03_09850"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNX84743.1"
                     /translation="MYRTHTCGELSLSNRGETVSLAGWVQRSRDLGGMTFIDLRDRYG
                     ITQLVFNMETNADLCMKARKLGREFVIRAEGIVAERSNKNQKIPTGEIEIITERIEVL
                     NASDTPPFTIEENTDGGDELRMKYRYLDLRRQNVRSNIILRHKMAQEVRKYLDGQGFI
                     EVETPVLIKSTPEGARDFVVPSRLHHGEFYALPQSPQTFKQLLMVAGFDKYFQIVKCF
                     RDEDLRADRQPEFTQVDCEMSFIHQEDILNMFEGLARHLFQEIKGLVFHGPFPRISYD
                     EAMSRYGSDKPDIRFDMCITEITENVKGRNFPPFELAAYAGAVSAPGCAGWSRKQLDE
                     LTEFVKRPQIGAAGLVWAKVEADGTVRSSADKYYSADDLANIALMTGSDAGDLILMMA
                     GPRERTLSALGELRLEMGRRLGLRDSSVYAPLWVVDFPLLEWDDETQRFYAKHHPFTA
                     PKPEDIQYMESDPWRVRANAYDLVINGTEIGGGSIRIHDPETQSLMFRTLGFTAEEAQ
                     KQFGFLLNAFRYGAPPHGGIAFGFDRWTAIMGGSDTIRDYIAFPKNNQARDLMIDTPA
                     TIKQEQLDELGIEISKEDEKK"
     gene            5233..6090
                     /locus_tag="PKK03_09855"
     CDS             5233..6090
                     /locus_tag="PKK03_09855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNX84744.1"
                     /translation="MTTPLKTRTARLSVISNSLLIAMKLAAGIISGSVSIISEAIHSS
                     LDLVAAIIAFFAVKISGTPADERHPFGHGKVENVSGVIEALLIFAASAWIVYEAVDRF
                     IHPGETGALGLGVLVMAISAVVNTFVSRRLYKVARQTDSVALEADALHLKTDVITSAG
                     VALGLALIWLTGWHVLDPIIALGVAVIIIYESWMLLRKAFFPLLDTALSPEEMKKLTG
                     ILDEMQVSYHDLKTRAAGHQRFVEFHLDVPAQDTIDTIHRQCDLIEERLEREFRDIDV
                     IIHPEPKNS"
     gene            6129..6578
                     /locus_tag="PKK03_09860"
     CDS             6129..6578
                     /locus_tag="PKK03_09860"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNX84745.1"
                     /translation="MEPGSDEYYMKMALAEAEKAAARDEVPVGAVVVAGGTVIARAHN
                     LTETLNDPTAHAEMQAITAATSYLGGKYLTGCTIYVTVEPCVMCAGALAWSQVTALVW
                     GASDPKKGYTTTGAGILHPKTEVRTGVLAGKCSDIMKKFFRKKRDQA"
     gene            6638..7120
                     /locus_tag="PKK03_09865"
     CDS             6638..7120
                     /locus_tag="PKK03_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84746.1"
                     /translation="MTLDNGKKIVSLRLTGFITTVVYVLYIFLAYFPKVFRPIMTEKS
                     LTILTVVLTAAYLILLMRPVILKYMYLLFSADERKITLRWYKPGLMPGESKSIEIPVE
                     RFAGYEIKEQFMGLHSYLTLFQQVQGRKAAYPPVSISALTRKQREDIEATLKNYKSVI
                     "
     gene            7231..8568
                     /gene="gdhA"
                     /locus_tag="PKK03_09870"
     CDS             7231..8568
                     /gene="gdhA"
                     /locus_tag="PKK03_09870"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNX84747.1"
                     /translation="MKVKEILAELEKKHPGEPEYHQAVKEVLETIEDVYNENPRFAKA
                     GVLQRLVEPDRIFTFKVPWMDDKGNVQVNLGYRVQFNGAIGPYKGGLRFHPTVNLSIL
                     KFLGFEQIFKNSLTSLPMGGGKGGSDFDPKGKSENEVMRFCQSFMLELWKHIGPETDV
                     PAGDIGVGGREIGFLYGMYKKLAAEHTGVLTGKGLNWGGSLVRPEATGFGGIYFVKEM
                     LATKNDSLQGKKVSISGFGNVAWGAALKATQLGATVVTISGPDGYIYDPAGISGEKID
                     YMLDLRASNQDIVEPYAKKFAGSKFVKGKRPWEVKVDIALPCATQNELNGADAQTLIA
                     NKVMLVAEISNMGCTPEAIAEFHKHKIQYAPGKAVNAGGVATSGLEMTQNSMKLNWNK
                     DEVDEKLHSIMVNIHDACVRYGKRADGYVDYVKGANVAGFLKVANAMVDQGLV"
     gene            complement(8673..9782)
                     /gene="dprA"
                     /locus_tag="PKK03_09875"
     CDS             complement(8673..9782)
                     /gene="dprA"
                     /locus_tag="PKK03_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNX84748.1"
                     /translation="MSSMPLVYKIALGMIPGVGDITARRLVSYAGDVEAVFRESFRSL
                     TRIPGIGEGLASAIAGHEYLPAAEREAEFVARNNIRVYFYLDDDYPARLRQCEDSPVT
                     FYFRGNADLDSPMILSVVGTRHATENGRGLCHKIVSGLAEHFPELLIVSGLAYGIDIA
                     AHRAALAAGLPTVAVLGHGLKTIYPSVHAGVAKAMLEKGGHVTDFSSPTLPERNNFIR
                     RNRIIAGISDATLIIESGIKGGALITADIAASYNRDVMAVPGRPGDEWSAGCNSLIRC
                     NKASLVEKCAHIEYLLGWKPASLSAPVQPMLFTSLTDNEKRICEALRNDGGMTIDMLA
                     RMIDIPVYKLSPTLLQMELAGLIIPCPGNVYRLRG"
BASE COUNT         2553 a         2593 c         2522 g         2220 t
ORIGIN      
        1 atttcttatt gttagcgcat ttcaacccaa caatcagaat acacttccgt cccgtcagtc
       61 acactgacat ccgcactgtg gcatgccggt aaaaaagtac ccggcctgcg attaagggcc
      121 gggcatatcc gattcaaatc accagaaatg acaaaacctg ttgcaacgtc aacagttagt
      181 tcatcgaaat aaaaaatgct cagcctttaa aacagctcta aatttaagaa taaagaaata
      241 cacctatttt tttatttgac caaaaggggt atcgcataga caaaattgta tatgtgttgg
      301 attaactttc ggggtattgt ccgcatacca ccgcaagaca atctattttt caatttttaa
      361 tcaacaatgg ttgctttttc aattcacaga tactattttt agcctcactt gccacgggat
      421 tgggccctgg gctgtaaagt gtttaaaatc atattgataa agccatgaca aaaggactcg
      481 taacaaagaa ggacaaaaag aaagagcctg caaagagcct gaaagagaag cgtaaggaga
      541 agaaggaaaa gaagaacgca aaataaaatc gtaccctgac tttgcctgct ccgcctccgg
      601 agaatacaaa gccagctctc catacaactc ctcatattca ttacggcggc tcacctccgg
      661 tgacgccggg gggagtgttg tgttttttgc tgtagcggca caataatcgc cttgatttca
      721 gggttgatta aatacatttt tcttaacttc atgccccggc tgaattctat catcaggaac
      781 ccgatgaaaa agaagaccaa taaatcaccg ttcccctgga actccgtgag cgtgcttctt
      841 ttcaccgccg cagtcctcac agccatcatc attatctatt cgggagaaca gaagaaaaga
      901 ctgcttcgcg atgccgtcac tgaaatggag gccgttgcaa acctgaaatc agaacaggtg
      961 tctgaatgga aaaaagagcg tctcaatgac gcaacactga tccatgagaa cgtcaccctg
     1021 gttgagcata ttgacgccat gttcatcaag gacaactctg tccgtgaaaa atcaagactc
     1081 atccagctcc ttaccaccct gattgaaagc tacgacttcg gaggggcagt actgcttgac
     1141 gcaggaggaa gccccggaat agccgtcccg cctgaagaag cagtgatggg agaatacctc
     1201 agaggcaatg tcaccaaagc catttccaat ccggtcatat ccatgtctga tttccacacg
     1261 gcgaaagtgg tcaaatacct tcacctcgac ctgctcatcc ctatgaagat gccctatgag
     1321 ggcgacacgg tactcgccgg cgtgctggtc ctgagaatag atcccgaaaa ggaacttttc
     1381 ccgatgctct ccacctggcc aacaccggga agcacgtcgg agtgcatcct gttccgattt
     1441 gagggtgact ccgtaatcta cctcaacggc tcacaccttg caggatatga cagacctgtc
     1501 accttcgccg caggcccgat ggaactgagc gccatgaggc ccgccacgga atcagacgag
     1561 gcaaatgtct ataatgacta taccggtacc gaggtgatcg cttcggtcaa aaaagtgccc
     1621 ggcaccgact ggtacctcat agccaagaag gaaacctcgg agatccttgc caggttcatg
     1681 cgcgagataa ccatgctctg gatgatcatg ggcatagtct acctggccct tgccagcatg
     1741 gcatggctga tcataaaatc gaccaaagcc cgctacctcc gcgaaaggca acaggaagaa
     1801 ctgaaccggc aggccatcat gaaacacttc gatttcgtga tgaaacacgg gaatgacatc
     1861 atcctgctga ttgacagcga tctgaatatt gtcgaagcaa atgagcgggc tgtcaccaca
     1921 tatggctatc cgcgcgagaa actcatcggc atgaatgttt ctgagatccg tactccacac
     1981 gagataccgc agctgtcaga acatgtcagg aatctccagg agacaggctc atcatatatt
     2041 gaaaccgttc acctgcgtag tgacggaagc acgttcccca tcgagatcag cgcctatact
     2101 gttgaggtag aagggaacag gttcttccag agcatcggcc gtgacatcac tgaccgtaaa
     2161 cgtaccgagg aggagatgcg cgagagcaac aaaaaactca gcaccatcat caataacctc
     2221 cggggagtgg tgttccgctg caacaacgac cggaactgga ccatgcagta catcagcgac
     2281 ggcatctacg agcttgccgg ctatctgcct aatgagttca ttaacaataa aataagaact
     2341 tacagttcga taattgaccc ggaagatgtg gagagagtct ggaataacgt ccatgctgcc
     2401 ctggaaacag ggtaccttta taccatagaa tacaggatca tcacctcatc gggaaacagg
     2461 aaatgggtct gggagcgcgg acgaggctat tacgaaagcg acaaactggt agccctcgaa
     2521 ggcttcattt cagacatcac cgaacggaaa cggatagagg aagaactcat cagggcaaag
     2581 gaaaaggcgg aacagagcga caggctgaag acggcattcc tgcataacat ctcgcacgag
     2641 atccgtaccc cgatgaacgc tatcgtcgga ttcacaaccc tgcttgactc gcctgacacc
     2701 acggatgatt cgcgcaggca atatattgac ataatctatc agtcgagcaa ccagctgctc
     2761 tcaattatca ctgacatcgt cgacatctcc aatattgaga ccggcctggt aaagatcagc
     2821 cggtcaaccg tcaatatgaa ccagctgatc aggagcctct atgaccagta ccggatgaga
     2881 gtccagcaac agggtctcgt gctcaacttt accacacacc ttgatgaccg ggatgcacag
     2941 gtggtaacgg atgaaacaaa ggtgatccag attttttcca acctgctcaa caatgccctg
     3001 agattcacca aaaagggaag gatagagttg ggatacgtgg tcaggggtga aatggtggaa
     3061 ttctttgtat ctgacaccgg tatcgggata gcccccgagt tccagcagaa ggtctttgag
     3121 agattcttcc aggtggaagg acccagttcg aagcagtaca gcggaacagg cctcggtctg
     3181 agcatttcga aggcatacgc cgaacttctc ggtggctcaa tatggcttat ctcaacgccg
     3241 ggagaaggat cgctgttctg tttctccatt ccattcgcca ggccggcgga accggtggtc
     3301 caggccagga catcttcaca caagtgaccg gctgaagccg ggaaggaggc tactttttct
     3361 cgtcttcctt actgatctct attcccagct cgtcaagctg ctcctgcttt atcgtggcag
     3421 gggtgtcaat catcaagtca cgggcctggt tgtttttcgg gaaagcaatg taatcacgga
     3481 tggtatccga gccacccatg attgccgtcc acctgtcaaa cccgaacgca atcccaccgt
     3541 gcggcggggc gccgtagcgg aaggcattga gcaggaagcc gaactgcttc tgcgcttcct
     3601 cggcagtgaa gccaagagtc cggaacatga gcgactgtgt ttccgggtca tggatacgta
     3661 tcgagccccc tcctatctcc gtcccgttaa ttaccaggtc ataagcgttg gcacgcactc
     3721 tccacgggtc gctttccata tattggatat cctccggctt aggtgcggta aacgggtgat
     3781 gcttcgcgta gaagcgctgc gtctcatcgt cccactccag cagcgggaag tcgaccaccc
     3841 acaggggagc atagactgag ctgtcgcgaa ggccaagacg cctgcccatc tcgagacgaa
     3901 gttcaccaag ggcggatagt gttctttccc tcggaccggc catcatcagt ataaggtcgc
     3961 cggcatccga accggtcatc agggcgatgt tggcaaggtc gtcggcgctg taatacttgt
     4021 cagccgatga tctgaccgtt ccgtctgcct cgaccttcgc ccatacgagg ccggcagccc
     4081 ctatctgtgg gcgcttgaca aactcggtca gttcatcgag ctgcttgcgc gaccagcctg
     4141 cacagcccgg ggcggaaaca gcccccgcat aggcagcaag ttcaaaaggc gggaaattac
     4201 ggcccttgac gttctcagta atctcggtaa tgcacatgtc aaaccttatg tcaggcttat
     4261 cactgccata ccggctcata gcctcatcat acgaaatgcg cgggaaaggc ccgtgaaaca
     4321 ccaatccctt tatctcctgg aaaagatggc gggccagccc ttcaaacata ttcaggatat
     4381 cttcctggtg gatgaatgac atctcgcagt caacctgggt gaattccggc tgccggtctg
     4441 cacgcaggtc ttcatcacgg aagcacttta caatctggaa atacttgtcg aatccggcca
     4501 ccatcagcaa ctgcttgaat gtctgcggac tctgcggaag ggcataaaac tctccgtggt
     4561 gcagccttga aggcacaaca aaatcgcgcg ctccctcggg ggttgacttg atgagcaccg
     4621 gtgtctccac ctcgatgaat ccctggccgt caaggtactt gcgcacctcc tgggccattt
     4681 tgtgcctgag aatgatgttc gagcgcacgt tctgccttcg caggtcaagg tacctgtatt
     4741 tcatcctcag ttcgtcaccg ccgtcggtgt tctcctcgat cgtgaagggc ggggtgtcag
     4801 atgcattgag cacctcgatc cgctcagtga ttatctctat ttcgcccgta gggatcttct
     4861 gattcttatt gcttcgctca gcgacaatgc cttcagcacg gatcacaaac tcacggccca
     4921 gtttacgggc tttcatgcaa aggtcagcgt tagtctccat gttaaagacc agctgggtga
     4981 tgccgtacct gtcacgcagg tcaatgaacg tcattccccc gaggtccctc gaacgctgta
     5041 cccatccggc cagactgacc gtttctcccc tgttgctgag tgaaagctca ccgcaagtat
     5101 gtgtcctgta catatcagat attttgagga gacaaaaata aggtattccg gtaacaaacg
     5161 gtaataactt tcctctgact tgtcgctcct ttttcctaaa attgcagcag ttcaaaagca
     5221 gttccgacag caatgaccac tccactgaag acacgtactg caaggctttc ggtcatatcg
     5281 aacagcctgc tcatagcgat gaaactggcc gcagggatta tttccggttc ggtcagcatc
     5341 atttccgagg ccatccattc atccctggac ctggtggcag ccattatcgc atttttcgca
     5401 gtaaagatat cggggacacc tgctgatgag aggcatccct tcggccacgg aaaggtggag
     5461 aatgtctcgg gcgtgattga ggcgcttttg attttcgccg cctcggcatg gattgtatat
     5521 gaggcagttg acaggtttat acatcccggg gagacgggag cgttgggcct gggggtgctg
     5581 gtgatggcca tctctgctgt tgtcaacaca tttgtctcca ggcgtctgta caaagttgcc
     5641 cggcagactg actccgttgc cctggaagct gatgccctgc atctcaagac tgatgtaatc
     5701 acatcggccg gtgtggcgct cgggctggcg ctgatctggc tgacaggctg gcatgtgctt
     5761 gatcccatca tcgccctggg cgtggccgtc ataataatat atgaatcatg gatgcttctc
     5821 agaaaagcct ttttcccgct ccttgacaca gccctctcac cagaggagat gaagaaactg
     5881 acaggcatac tggatgagat gcaggttagc taccatgacc tgaagacaag ggctgccggc
     5941 catcagaggt ttgttgagtt ccaccttgat gtgccggccc aggatacaat tgacaccatc
     6001 caccgtcagt gtgacctgat cgaggagcgc ctggaacgcg aattcaggga catagacgtg
     6061 atcatccatc ccgaaccgaa aaattcgtaa gactgcaatc tcctgtaacc tgaagacaga
     6121 acaccaccat ggaacccgga agcgatgaat attacatgaa gatggctctt gccgaagctg
     6181 aaaaggctgc cgcccgtgac gaggtgccgg tgggggcagt ggttgttgcc ggaggcacag
     6241 tgattgcccg tgcccacaac ctgacggaga cactcaatga tcccaccgcc catgcagaga
     6301 tgcaggccat cacagccgct acttcttacc tcggtggcaa ataccttacc ggctgtacca
     6361 tttacgttac cgtggagcca tgcgtcatgt gcgccggtgc gctggcatgg agccaggtca
     6421 ctgcactggt atggggtgca tccgatccga aaaagggcta taccaccaca ggcgccggta
     6481 tactgcatcc gaagacggag gtccgcaccg gagtacttgc cggaaaatgc tcggacataa
     6541 tgaaaaagtt cttcaggaaa aagagggacc aggcctgaca gctgcccccc cctccgattc
     6601 gaaaaaaatt agtaggtttg tttaaaacct gacactcatg acccttgaca atggcaaaaa
     6661 aattgtttcg ctgaggctga ccggattcat caccacggtg gtgtatgttc tgtacatctt
     6721 cctggcctat tttccaaagg tattcagacc tataatgaca gaaaagagcc ttacaatact
     6781 gacagtcgtg ttaacggcgg catacctgat tttgctcatg cggccggtga tactgaagta
     6841 tatgtacctt ttattttcag ctgatgagcg aaagataacc ctccggtggt ataagccggg
     6901 gctgatgccc ggcgaaagca aaagcattga gataccggtc gagaggtttg ccgggtatga
     6961 gattaaagaa caattcatgg gcctgcatag ctacctcacc cttttccagc aggtacaggg
     7021 gcggaaggca gcataccctc cggtgagtat ttcagcactt acaaggaaac agagggaaga
     7081 tattgaggca actcttaaaa actacaaatc agttatttag acggtctccg gtaaaacatg
     7141 agcaaagtca tttttcatga ctggcggttc attgaccttt gaggagtaat agatctttac
     7201 aataatcaca aaccacaaac atacattgaa atgaaagtaa aagagattct ggctgagctc
     7261 gagaagaagc atcctggtga acccgaatat catcaggcag tcaaggaggt ccttgaaact
     7321 atcgaggatg tttacaatga gaaccccagg tttgccaagg caggtgtcct gcagaggctg
     7381 gttgagccgg accgcatatt cacctttaag gtaccctgga tggatgacaa gggcaatgtc
     7441 caggtcaatc tcggctacag ggtgcagttc aacggagcca tcggcccata caagggcgga
     7501 cttcgcttcc accccacggt gaacctttca atccttaagt tcctgggctt cgagcagatc
     7561 ttcaagaact cccttacctc cctcccgatg ggtggcggca agggcggatc agacttcgat
     7621 cccaagggca agtcagaaaa cgaagtgatg cgtttctgcc agtcgttcat gcttgagctg
     7681 tggaagcata tcggtcccga gactgacgtt ccagcaggtg acataggtgt aggcggacgc
     7741 gaaatcggtt tcctgtacgg catgtacaag aaacttgcag ctgagcacac cggcgtactg
     7801 acaggcaagg gcctgaactg gggcggttct ctcgtacgtc ccgaggccac aggcttcggc
     7861 ggtatttact ttgtcaagga gatgctggct acaaagaatg acagtctcca gggcaagaag
     7921 gtatcaatct ccggcttcgg caacgttgca tggggtgcag ccctcaaggc tacccagctt
     7981 ggtgcaacgg ttgttacaat atccggtccc gatggttata tatatgatcc cgctggtatc
     8041 agcggagaga agattgatta tatgcttgat ctgagggcat cgaaccagga tatcgttgaa
     8101 ccttatgcaa agaaatttgc cggctcgaag ttcgtcaagg gaaagaggcc atgggaagta
     8161 aaggtagaca tcgcactgcc gtgcgcaacg cagaatgagc ttaacggtgc cgatgcccag
     8221 accctgatcg ccaacaaggt gatgcttgtt gccgagatct cgaatatggg ttgcacaccg
     8281 gaggccatcg cagaattcca caagcacaag attcagtatg ctccgggcaa ggcagtaaac
     8341 gccggcggtg tagccacctc gggtcttgag atgacgcaga actcgatgaa gctcaactgg
     8401 aacaaggatg aggttgatga gaagctgcat tcgatcatgg tcaacattca tgatgcatgc
     8461 gtcaggtacg gcaagcgtgc cgacggttat gtagattatg tcaagggtgc aaacgtcgca
     8521 ggcttcctga aggttgccaa cgcaatggtt gaccagggat tggtataacc accgagatta
     8581 aacattgatg caggttatct gcattttatc acgtaacgac gggtctgaga cccgtcgtta
     8641 cctgcatata tgaacgataa tccttgtcat tgtcatccac gtaaccggta tacattcccc
     8701 gggcagggta ttatcaaccc ggcaagctcc atctgcagca gggtgggtga cagcttatat
     8761 accggaatat caatcatccg ggcaagcatg tcaatggtca taccgccgtc attcctcaac
     8821 gcctcgcaga tccttttttc attatccgta aggctggtga acagcatcgg ctgaacgggc
     8881 gctgacagcg atgccggctt ccagccgagg aggtattcaa tgtgagcaca cttctccacc
     8941 agggaggcct tgttgcacct tatcagtgaa ttgcagccgg ctgaccattc atctcccggc
     9001 ctgccgggaa ccgccatcac gtcacggtta tacgaagccg cgatgtcagc ggtgatgagc
     9061 gcccctccct ttatccccga ctctatgatc agggtggcat cagagatgcc tgcaatgatg
     9121 cggtttcgcc tgatgaagtt gttacgctcc ggcaacgtcg gtgaggagaa gtctgtgaca
     9181 tgtccgccct tctcaagcat cgctttagct acgcccgcgt ggaccgacgg atagatggtc
     9241 ttcagtccgt gacccagtac tgcgacggta ggcagccctg ctgcgagggc agccctgtgg
     9301 gcagcgatgt caattccgta agcgaggccg cttacaatca gcaactcagg aaaatgctcc
     9361 gccagtccgg aaacgatctt atggcacaac ccgcggccgt tttcagtggc atgccttgtg
     9421 cctaccacgc tgagtatcat cggcgagtca aggtcagcgt taccccggaa ataaaaagtc
     9481 acgggagaat cttcgcactg tctgagccgc gccgggtaat cgtcgtcaag gtaaaagtat
     9541 accctgatat tgttcctggc gacaaattct gcttcccgtt cggcggcggg gagatactca
     9601 tgaccggcaa tagcggaggc aagcccctcg cctattcccg ggatcctggt caggctgcgg
     9661 aacgattccc tgaagacggc ttccacgtcc ccggcgtatg aaaccagcct gcgggcggta
     9721 atgtcgccca caccgggtat catgccaaga gcgatcttgt aaacaagtgg catcgatgac
     9781 attgtgcaat actttagcga gacactataa agataggaac aaaaaggcaa atgatataaa
     9841 tttgtgccat gcaaatcatt gacactcata accacatata cctgcctg
//
LOCUS       DAOPXU010000105         9791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_8141_length_9791_cov_12.134449, whole genome shotgun sequence.
ACCESSION   DAOPXU010000105 DAOPXU010000000
VERSION     DAOPXU010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9791
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8141_length_9791_cov_12.134449"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="PKK03_09880"
     CDS             <1..1187
                     /locus_tag="PKK03_09880"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX84749.1"
                     /translation="DRNDGQGRCDIYFSSYDGNRWSPGINVGPPVNTTYWESQPSVSP
                     NGRMLFFVSNRPGGIGGMDIWYSLRGSDGRWGKPVNPGKVINTVDDEFSPFIYFNGRT
                     LYFSSNGRESFGGHDIYVSTMNRDSTWTSPENLGPPVNTPADETGLVIESSGRRAYFS
                     SVRDKGRGKDIYYLDLPQQVQPEQVLYFQGTVIDKASGQPIVAKYELSDLTNGIDVIT
                     SLTDNRGGFFVCLPRGSSYGMNVTADGYMIYSENFDFAEGYTSAEPYRKTIALNRVRE
                     GEFMRMYNVFYDTDSWELLETSRPELEHLLEFLQVNHTVVIEIGGHTDSDGSDEHNQR
                     LSERRADSVREFLMNRGIDGKRIFAHGYGETHPVADNITPAGKRLNRRTEITILSEGI
                     AR"
     gene            complement(1305..2120)
                     /locus_tag="PKK03_09885"
     CDS             complement(1305..2120)
                     /locus_tag="PKK03_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNX84750.1"
                     /translation="MFEILRNAWNETGLTAANGHEKLDKMINQPNILSGVSHEVRTQM
                     NSIVAFSYLINNERFSESERREFSEQIIISCEQLIGLFENFFDTAALESGSARENLRE
                     TDITRLFESLTSDFRVFMRKKGRDNVVLIQEDNLPEKLMLKLDTARMTRIVSNLFRNA
                     LDNTLSGFIKVGCTWYDDTITFYVKDSGQGYPRSSDLLLSDNPEIQHSESQDTYSAMN
                     LILARNLILAMDGRIRVETNDAGGTSVFVSLPARESPDLKILTGTSSENKIAI"
     gene            complement(2148..2894)
                     /locus_tag="PKK03_09890"
     CDS             complement(2148..2894)
                     /locus_tag="PKK03_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84751.1"
                     /translation="MKTKDVNNAIENWLGGHADRSAQDWLIDEMKKDAALAREVTLRR
                     KTDEILSDREVINLRHKLTAIEMRRRNAGTVRRTVIRSARYAAAVALIAILSTALYLT
                     LRPSDSPGELYSANYSRYESPGAVRSAISSENTLMENAIASYAAREYDKAIVYLEQLI
                     NTGQEDMESVFMHGMANMEVKNYPVASGSFTRVIDHNDNLYLEDAAWYLGLCYMMTDN
                     KDKAVRQFSTIASSGSRYSKQAARLVRRLK"
     gene            complement(3086..3220)
                     /locus_tag="PKK03_09895"
     CDS             complement(3086..3220)
                     /locus_tag="PKK03_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84752.1"
                     /translation="MKTLTIGNFSEFILSNEEMINVRGGNGANNEVGDPTKPPVIIEI
                     "
     gene            3408..6590
                     /locus_tag="PKK03_09900"
     CDS             3408..6590
                     /locus_tag="PKK03_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNX84753.1"
                     /translation="MEERRTTLMRPKSGIITLIVLFLMVVCMVPDTVLAAGAGKQTIA
                     GFYKEESSVGWRESGSAPFSAADIQWNAYEEERLREKTDSLAAYLEKRDRGADPVKLA
                     AEITAVLEASEVKDTLLLSDACYFVGVQLYQSNLYIKASDYFSRSVMYREKLDLADKR
                     YANGLINMAVSLLSSGDFYKAYPLGMKALDAKRAVSGSDSSGLATNYLNLASICLELN
                     DNEKAVTLAEAGLTVSGIYPDLVLPRVRADLYHVIGLSLYRTQEYNKSLVYCREALKI
                     FETYPAGNAASRILLHNTVALVYRRLGQSDKAEEYFRRGLAIKDEKNAQDKYLLYINY
                     SNLLGQSGQISEGERVLEEGIARVRNIYGPESREYFMMLVSFADFINSTRGGSARSLA
                     LYEQCFSYVRANPWDVSVSKFFTVKYAQSLANAGKYAEVLRITDEMEFLTGAHSPAAG
                     AEAGSGGNLAAPAVFSEDDVDILEIRYAALDALANVAHDSHYDRQAVATGRKITSLYD
                     RQRLELSEDESRTSFSTYSRDLYTGMIGNYVRLYEADHSREALEGIFEYSERSKVAGF
                     LASIRELNAARFSLPAELAGLEAEIRRQTGFYRELIASEQLKAVPDSQRLATWESVTF
                     RLLRSRDSLNRIFEEQYPSYYNLKYRNEVASPGDVGKVIGRRSNLLSYVLTSDRLYIL
                     VANSRGNKVITREIDSGFFDTLHRFRGILSTTPRTSGSRAPFNEYMDMAYSLYRILIE
                     PAEPYLVGDKIVISPDNILSYLPFETLVTEEFRSPGLLYREAPFALKKYRFSYIYSVT
                     LSSETMQRTRRFNNKLVAFAPTYEGMEISDSVLAAWPTLRGEIRELPAALIEAEDAVS
                     QCGGRAYLAREASEETFKREAPGYDIIHLAMHTLVDDSRPAFSKMLFAGSEDSRDDGM
                     LNTYEVYSLPLKAMMVVLSSCNTGSGILAGGEGILSLARGFLYAGSRSAVMSMWEVDD
                     ASASEVIHSFYKNMRSGQTKSSALRNARLKFLRTADQGRSHPYYWSALVIYGDDTPLW
                     YNRVTLYISLLLLLLVATALVALVYRGPRS"
     gene            complement(6569..7135)
                     /locus_tag="PKK03_09905"
     CDS             complement(6569..7135)
                     /locus_tag="PKK03_09905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNX84754.1"
                     /translation="MGLFRKYSDLDIIDGVRRQDNRILSYLYDSYYEMVSDYVRKNSG
                     TEDDAGEVMQESVVNLYRQVTTGEFSVKADLKGYFFGMARNVWNAQLRHRSRLVHFET
                     EPADHSSSEDLTRATLERIVARSFALLAEDCQTVLTLYGDGLSYEEIASRMGLKNAAY
                     ARRKKYLCKEALMEIIKTDPEFHDLGPL"
     gene            7275..9461
                     /locus_tag="PKK03_09910"
     CDS             7275..9461
                     /locus_tag="PKK03_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HNX84755.1"
                     /translation="MRFPFVRQHDAMDCGPACISMVAMCHGKRLSLETIRRKAWITRE
                     GVSFLGLKTAAESIGFRVAGVKIPFSRLREEAPLPCIAHWHQNHFIVINRIGEKAVWV
                     SDPAIGRIKMTLDEFRQGWISGEAAGEPAGMALLLEPGPGFEALHDDPPPKGGFEFLL
                     PYLRPYRKQIIFLIAGLLVSSAIQLVFPFLTQAIIDRGIAGRDIKFIWLVLSGQLALT
                     AGRLAVEFTRGWLLLHMGTRLNINIVSDFLARLMGLPIAYFDTKLNGDILQRIDDNSR
                     IENYLTSSSLAILFSLFNFVVFGVVLGIYSTRVLLIFLAGTALYVLYVTLFMPARERL
                     DNMRFIQMAEAGNRMINIVNGMQEIKLAGNEATNRKEWEAQQHAIYNTRIRGLRILQF
                     QTAGGTLIHESVNIFITVISATLVINGSMTLGMMLAVQFITGQLNGPVSQIVNFMRAT
                     QDARISLERLAEVHAMAPEEESVAADAAEMPEKADVEVRNLSFRYEGPGSPWVLRNLD
                     LSIPAGKVTAVVGESGSGKTTLLKMLLGFYRAEEGDIYIGGTRLEDIRISSLRKNTGA
                     VMQEGYIFPDTIMGNIAPGVENPDAEKVAEAVRVARLKPMLDMLPSGLQTRVGQGGHG
                     LSQGQRQRILIARVVYRKPALLLLDEATSALDASNEKMIVENLAEFYAGRTVVVVAHR
                     LSTVRHADNIVVLEAGRVAESGTHASLTAMKGTYYRLIRDQLEMGR"
     gene            complement(9480..9662)
                     /locus_tag="PKK03_09915"
     CDS             complement(9480..9662)
                     /locus_tag="PKK03_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HNX84756.1"
                     /translation="MEKKDIIILVAAFVLAGFSLYRKYMKKKESSSGNTPLKKSGRSS
                     LKDQADDYEPYSGGKS"
BASE COUNT         2387 a         2354 c         2578 g         2472 t
ORIGIN      
        1 gtgacagaaa cgacggacag gggagatgtg acatctactt ctcatcatat gacggcaaca
       61 ggtggtcacc cgggattaat gtcgggcctc ctgtcaatac aacttactgg gaatcacagc
      121 catctgtcag tcccaacgga aggatgcttt tcttcgtcag caacagaccc ggggggatcg
      181 ggggtatgga catatggtat tcactgcgtg gcagtgacgg cagatggggc aaaccggtca
      241 acccgggaaa ggtgattaac acggtggacg atgaattttc acccttcatc tatttcaacg
      301 gaagaacgct ctacttctca tccaacggca gggagtcatt tggcgggcat gatatctatg
      361 tgtccaccat gaaccgtgat tccacatgga caagcccgga aaacctgggt ccgccggtaa
      421 atactcctgc tgatgaaaca ggacttgtga tcgagtcttc gggccgcagg gcttactttt
      481 cctcagtccg tgacaaggga agggggaagg atatatatta cctggatctg ccgcagcagg
      541 ttcagcccga acaggttctg tacttccagg ggacggttat tgacaaggcg tcagggcaac
      601 ccattgttgc aaagtatgaa ctgtcagacc ttacaaatgg tattgatgtg ataacttcgt
      661 taactgacaa caggggaggg ttcttcgtct gcctgccccg gggaagcagt tacgggatga
      721 atgtcacggc agacggttac atgatctatt ctgagaactt cgacttcgcc gagggctata
      781 catccgcgga gccttacagg aagaccattg ccctgaacag ggtacgtgag ggagaattca
      841 tgcggatgta caatgtgttc tatgacactg actcatggga gttgcttgaa acatcgcggc
      901 ctgaacttga gcatctgctt gaattcctgc aggtgaacca cactgttgtg attgagatcg
      961 gggggcatac tgactctgac ggaagtgatg agcataacca gcggctttct gagagaaggg
     1021 ctgattcagt cagggagttc ctgatgaacc ggggcattga cggaaagcgg atctttgcgc
     1081 atggttacgg tgagacccat ccagttgctg acaatatcac ccctgcgggg aagcgcctta
     1141 acaggcggac ggagataacg atactgtcgg aaggtattgc cagatgacgt atcggctgac
     1201 tgaagacctg tcacaatttg ggaattaata caaaccggta agggagggtg ccctggggca
     1261 gccctcctta ccactcttaa cagacagttg taggcttcag agaatcagat ggctatcttg
     1321 ttctccgaag aggtaccggt aagtattttc agatccggac tctctcttgc gggtaatgaa
     1381 acgaagacag aggtgcctcc ggcgtcgttg gtttcaaccc tgattctgcc gtccattgca
     1441 agaataagat ttctggccag gattaaattc atagcggaat aggtatcctg actttcggaa
     1501 tgttggatct caggattgtc ggaaagcagc aggtcgctgc ttctgggata accctggcca
     1561 gaatctttta catagaaggt gattgtatca tcataccagg tgcaccctac tttaataaac
     1621 cctgataaag tattgtcgag tgcattcctg aacagattgc ttacaatcct ggtcattctt
     1681 gccgtatcaa gtttcagcat cagcttttcg ggaaggttat cctcctggat gagaaccacg
     1741 ttgtcacgtc cttttttcct catgaaaacc ctgaaatcag aggtaagtga ctcaaacagc
     1801 ctggtgatat ctgtctcgcg gaggttttcc ctggctgacc ctgattcaag ggcagcagtg
     1861 tcaaagaagt tttcaaacaa tcctatgagc tgttcgcatg agattatgat ctgctctgag
     1921 aactcacgtc tttcgctttc gctgaacctt tcgttattga taaggtagga aaaagctacg
     1981 attgagttca tctgggttct cacctcatgt gaaacacctg aaagaatatt tggttggttg
     2041 atcatcttgt ccagtttttc atgcccattc gctgctgtca gccccgtctc attccaggca
     2101 ttgcgaagaa tctcgaacat atgaacagtt agttgtagtt tagttgatca tttaaggcgg
     2161 cggaccagcc tggcagcctg tttgctgtac ctgcttccgg aagaggctat agtactgaac
     2221 tgcctgacag ccttgtcttt attgtcggtc atcatatagc agagtccgag gtaccaggcc
     2281 gcatcttcaa gataaaggtt gtcattgtga tcgatgacac gggtgaatga tccgctggct
     2341 accgggtagt tctttacctc catgttggcc atcccgtgca tgaacactga ttccatgtct
     2401 tcctgccctg tgttaatcag ttgttcgaga tagacaattg ctttatcata ctctcttgca
     2461 gcgtatgagg cgatggcgtt ttccatgagg gtattctccg aactgattgc agacctgact
     2521 gcccctggtg actcatagcg tgaatagttg gcagaataaa gttctccggg tgagtcagac
     2581 ggcctcagcg tcaggtagag ggcagtggac agtatggcaa tcagggccac agctgccgca
     2641 taccttgcac tcctgatgac ggttctcctc acggttccgg catttcgcct cctcatctca
     2701 atagctgtca gtttatgtct gaggttgata acctcgcggt cagagagaat ctcgtcagtc
     2761 tttcttctca gagttacttc ccttgccagt gcagcatctt ttttcatttc atcaatcagc
     2821 caatcctgtg cagacctgtc tgcgtgcccg cccagccaat tctcgatggc attgttgaca
     2881 tcttttgtct tcatgttgta cattattatt tcacactcat ataaatctat cagtcagata
     2941 gttatttatg ttctgccccg tacttttgcg gacagtgccg ccggtatccg ggcgtgatgc
     3001 ctgacattca gggctatctg caaacgcgga tttacccctt ttatgttaca aaaacaggcc
     3061 gggtcaaccg gcaattataa tcagctcaga tctcgatgat caccggaggt ttggtcggat
     3121 caccaacttc gttatttgct ccgttgccac cgcgtacgtt tatcatttcc tcgtttgaaa
     3181 gaataaattc tgaaaagttt ccaattgtca gggttttcat aactgtaatt tttaaagtgt
     3241 taaaatcagg gtttaattat catttatacc gtacgtgtca gaaagtgcca ttttgtgaca
     3301 cgaaaaaatc aacttttttt taaaaaaaat ccaacatatt gaataacaag cttgtataac
     3361 atgggtaaaa tgcgaacccg gtaatggatt gttttatact tttaccggtg gaagaaagaa
     3421 gaaccacact gatgcgtccg aagtcaggaa ttatcacttt aatagtcctg tttttgatgg
     3481 ttgtctgcat ggtaccggac acggttctgg cagcaggagc agggaaacag actattgcag
     3541 gcttttataa agaagagagt tcagtcggat ggagagaatc cggctcagca ccattctctg
     3601 ctgctgatat tcaatggaat gcatatgagg aggaaaggct gagggagaag actgattcac
     3661 ttgcagctta cctggaaaag agggatcgtg gtgccgatcc ggtgaagctc gcggcggaaa
     3721 tcactgcagt gcttgaagca tctgaagtaa aagatactct tttgctatct gatgcctgtt
     3781 actttgtcgg ggtacagctt tatcagtcaa acctttacat aaaggcttct gattattttt
     3841 cgcgatcggt catgtacagg gagaagcttg atctggctga caagcggtat gccaacggtt
     3901 tgatcaatat ggcagtttcg ctgctcagtt ccggagattt ttacaaagct tatccactgg
     3961 ggatgaaagc tctggacgca aagagagctg tctctggaag tgactcttcc gggctggcaa
     4021 caaattatct caaccttgca agcatatgcc tggagctgaa tgacaatgag aaggctgtta
     4081 ccctggctga agctggtctg acggtatcgg gaatctatcc tgaccttgta ctgcccaggg
     4141 tcagggctga cctgtatcat gtgataggac tgagccttta ccgcacccag gaatataaca
     4201 agtcactggt ctactgtcgg gaggcgctga agatatttga aacttatcct gcagggaatg
     4261 cagcctcccg tattctgctg cacaacactg ttgccctggt ttacaggcgg ctgggacagt
     4321 ctgacaaggc tgaggaatac ttcaggagag ggctggcgat aaaggacgaa aagaatgctc
     4381 aggataaata ccttttatat atcaattatt caaatctgct gggtcagtcg gggcagatca
     4441 gtgaagggga aagggtgctg gaggagggta ttgcacgggt aagaaacatc tacgggcccg
     4501 aaagcaggga atacttcatg atgctcgttt cctttgctga cttcattaac agcacccggg
     4561 gcggcagtgc acgctcactg gctctttatg aacagtgttt cagctatgtg cgggccaatc
     4621 catgggatgt gtcagtgtca aagttcttta cggtcaagta tgcgcagtca cttgccaacg
     4681 cgggaaaata tgccgaggtt ctgaggataa ccgatgaaat ggaattcctg acgggagctc
     4741 attccccggc cgccggagca gaggcaggct ccggaggtaa tctcgcggca ccggcggtat
     4801 tttccgagga tgacgttgat atccttgaaa taaggtatgc tgccctggat gccctcgcga
     4861 atgttgcaca cgacagccat tatgaccggc aggcggtggc aaccggcagg aaaattactt
     4921 ccctgtatga ccgtcagcgc cttgaactga gcgaggatga aagccggacc agcttcagca
     4981 catattcaag ggatctctat acaggcatga tcggcaacta tgtaaggctt tatgaggcag
     5041 atcacagccg cgaagccctg gaagggatat ttgagtattc cgaaaggagc aaagtggcag
     5101 gattcctggc ttcgatccgt gagttgaacg ccgcacgttt ttcccttcct gctgagctgg
     5161 ccggtctcga agcggaaatc agacggcaga ccggttttta cagggaactg attgccagtg
     5221 agcagcttaa ggcagttcct gattcgcagc gactggctac atgggaaagt gtcaccttca
     5281 ggctgctcag gtcacgtgac tcgctaaacc gcatctttga agaacaatat ccttcttact
     5341 ataacctgaa atacaggaat gaggtggctt cgcccggcga tgtgggaaag gtcatcggca
     5401 gaaggtcgaa tctgctcagc tatgtcctga cgtcagacag gctttatatc cttgttgcca
     5461 acagcagggg gaataaggtt atcacccggg agatcgattc aggttttttt gacactcttc
     5521 accgctttcg cgggatactg tcaaccaccc ccaggacaag cggatcacgt gccccgttca
     5581 atgagtatat ggatatggcc tacagcctat accgcatact gatcgagccc gccgaaccat
     5641 accttgttgg tgacaagata gttatatcgc ctgacaacat cctttcctat cttccgtttg
     5701 agacgcttgt gactgaagag tttcgcagtc ccgggcttct gtaccgggag gctccgtttg
     5761 ctttgaagaa gtatcgtttc tcttatattt acagtgtcac cctttcatca gagacaatgc
     5821 agagaacccg gagattcaac aacaagcttg ttgcctttgc cccgacgtac gaagggatgg
     5881 agatctctga ttctgttctt gcagcgtggc ccaccctcag gggagagatc agggagcttc
     5941 cggccgccct tattgaggct gaagatgctg tcagccagtg tggtggcagg gcttacctcg
     6001 cccgggaggc cagcgaggag accttcaagc gtgaggcgcc cggttacgat atcattcacc
     6061 tggcaatgca taccctcgtg gatgacagcc ggcctgcctt ttcaaaaatg ctctttgccg
     6121 gcagtgagga tagccgtgat gacggaatgc tcaacacata cgaggtttac agcctcccgc
     6181 ttaaggcaat gatggttgtc ctcagctcat gcaacacagg cagtggcatc ctggcaggcg
     6241 gagaaggaat actcagcctt gcccgcggat tcctctatgc cgggagccga tcggcagtga
     6301 tgtcgatgtg ggaggtggat gacgcctcag cctcggaggt tatacactcc ttttacaaga
     6361 atatgagaag cggacagaca aagagttccg cactgcgcaa tgcacggctg aaattcctca
     6421 ggacggctga ccagggacgg tctcatccat attactggtc ggcgctcgtc atctacggag
     6481 atgacacccc cctgtggtat aacagggtca cactttatat ctcccttctt cttctgttgc
     6541 tggtggctac ggcgctggtg gccctggttt acaggggacc cagatcatga aattccggat
     6601 ctgtcttgat gatctccatc agggcttcct tgcagagata cttttttctc ctggcatagg
     6661 cagcattttt caatcccatc ctgctggcaa tctcttcgta agacagtccg tctccgtaga
     6721 gggtgagcac cgtctggcaa tcctctgcaa gaagtgcgaa tgaacgggca actattcgct
     6781 caagcgtagc cctggtcaga tcctcggatg atgaatgatc ggcaggttcg gtctcaaaat
     6841 ggaccagcct ggagcggtgc ctcagctgcg cgttccagac attcctcgcc atcccgaaaa
     6901 aatagccctt gaggtcagcc ttcaccgaga actctccggt ggtgacctgc ctgtacaggt
     6961 ttacaaccga ttcctgcatt acctcgcctg catcatcctc agtaccgctg ttcttcctga
     7021 catagtcact gaccatctcg tagtaggaat catacaggta tgacaggata cggttatcct
     7081 gccgcctgac tccatcaatg atgtcaaggt ccgagtactt cctgaataat cccaaacaag
     7141 cggtttaatt ggcaaatata caatttccgc aatcgataca gttaatatgc aggggcgcac
     7201 attttaagtt caacggcgtg agtcggcttt aattggatat atttacattt gcaatgagtc
     7261 attccttctg caagatgaga tttccgtttg tcaggcagca tgatgccatg gattgcgggc
     7321 ccgcatgcat cagcatggtg gcaatgtgtc atggcaaacg tctgtcactt gagacaataa
     7381 ggagaaaggc atggatcacc cgtgaagggg tatcattcct gggtctgaaa acagcggcgg
     7441 agagtatcgg gttcagggtt gcaggggtga agataccctt cagcaggctc agggaggaag
     7501 ctcctctgcc atgcattgcg cactggcatc agaatcattt catagtcatc aacaggattg
     7561 gtgagaaagc tgtctgggtt agtgaccctg ccattggcag gataaaaatg accctggatg
     7621 agttcaggca gggatggata tccggggagg cagccggtga gcctgcgggg atggcattgt
     7681 tgctggagcc cggacccgga ttcgaagcgc tgcatgatga tccgccgcca aagggcggct
     7741 tcgaattcct gctgccctac ctcagacctt accggaagca gataatcttc cttattgccg
     7801 gcttgctcgt atccagtgcc atccagcttg tcttcccctt cctgacacag gcaataatcg
     7861 acagggggat agccggacgc gatattaagt tcatctggct tgtgctgagc ggccagcttg
     7921 cccttactgc cggaaggctc gctgtggaat tcacacgggg atggcttctt ctgcatatgg
     7981 gtacaagact gaatatcaat attgtttcgg acttccttgc ccgcctgatg ggattgccga
     8041 ttgcctactt cgatacaaag ctgaacggcg atatacttca gagaatcgat gacaacagcc
     8101 ggattgagaa ttacctgacc tcttcaagcc ttgccattct cttttccctt ttcaattttg
     8161 tggttttcgg ggtggtcctc gggatctaca gcaccagggt gttgctgata ttcctcgccg
     8221 gcactgccct ttacgtactg tatgtcactc ttttcatgcc ggcccgcgag cgtcttgaca
     8281 acatgcgttt catacagatg gccgaggccg ggaacaggat gatcaacatc gtcaacggca
     8341 tgcaggagat aaagctcgcc ggcaacgagg ccacaaaccg gaaggaatgg gaggcacagc
     8401 agcacgcaat atacaatacc cggatcaggg gactgaggat acttcagttc cagaccgcag
     8461 ggggaacgct tattcacgaa tctgtcaata ttttcattac agtaatttca gcaacacttg
     8521 tcatcaacgg ctcaatgacc ctgggaatga tgctggcggt gcagttcatc accgggcagc
     8581 ttaacggccc cgtctcacag atagtaaatt tcatgagagc cacccaggat gcaaggatca
     8641 gccttgaacg gctggccgag gtgcatgcca tggcccccga ggaagaatcc gtggcagccg
     8701 atgctgcaga gatgcctgaa aaggcagatg tcgaggtgag aaacctctct ttcagatacg
     8761 agggaccggg ttcaccatgg gtccttcgca acctggacct ttccataccg gcaggcaagg
     8821 tcactgccgt ggtgggggag agcggatcgg ggaagaccac cctgttgaag atgctgctcg
     8881 gtttctacag ggcagaggag ggtgatatat acatcggagg tacgaggctg gaagacataa
     8941 gaatctcatc actaagaaag aataccggtg ccgtaatgca ggaggggtat attttcccgg
     9001 acaccataat gggcaacatt gcccccggag tggagaatcc tgacgctgaa aaggtggcag
     9061 aggcagtaag agtggccagg ctgaagccca tgcttgacat gctgccatca ggactccaga
     9121 cccgcgtcgg gcagggaggc cacggtctca gccagggtca gcggcaacgg atcctgattg
     9181 ccagggtcgt atacaggaaa cccgcgcttc ttctccttga cgaggccaca agcgctcttg
     9241 acgcctcgaa cgaaaaaatg atcgtcgaaa acctggcgga attctatgcc gggcgaacag
     9301 tggtggttgt ggctcacagg ctcagcacag tgcggcatgc agacaacata gtcgtgcttg
     9361 aggcaggcag ggtggccgaa tcgggaacac acgccagcct taccgccatg aaaggtacgt
     9421 attacaggct tatcagagac cagcttgaga tgggccggta gaccggctct tcaccgctct
     9481 cagcttttcc cgccggaata gggttcgtaa tcatccgcct ggtccttcag gctgcttctg
     9541 ccagattttt tgagaggcgt gttgccggat gagctttctt tcttcttcat gtacttcctg
     9601 taaagagaaa agcctgcaag cacaaatgcc gcaacaagaa ttatgatgtc tttcttttcc
     9661 atggtagatg atcgtgaatc tgacaggcaa aaataaacaa tttgaagctc tgccggcatt
     9721 gcgcctcatt taaatggggt attggtttga gttttaccaa taatccatta ttttcgtggt
     9781 ttgaaaaaaa g
//
LOCUS       DAOPXU010000106         9701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_8235_length_9701_cov_13.521252, whole genome shotgun sequence.
ACCESSION   DAOPXU010000106 DAOPXU010000000
VERSION     DAOPXU010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9701
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8235_length_9701_cov_13.521252"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..1068
                     /locus_tag="PKK03_09920"
     CDS             55..1068
                     /locus_tag="PKK03_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX84757.1"
                     /translation="MKAPGILLSLLLLALTAVSCVTGKRYNTLTDRNLLLMSERDSLK
                     AENIWLTMSNRELTTKSMQLAGDAERLTREVEKARTDLAQSRDDVNRLSTRYDELQRA
                     QAELVAGKAQETQRLLADLRQAQTQLQQREDLMRDLEMNLDTKKATLEELTYELEKRN
                     TRLAELEKMLDAQQKAVKALKDKVSEALFGFENNGLTVTIKNGQVYVSMDDKLLFRTG
                     SYEIDANGRSALRKLGALLEKNTDVTITIEGHTDDVPYRAAPGQQILDNWDLSVKRAT
                     TVVRVLTQDTHINPKRFVASGRSEYIPVDPAKTPDARQKNRRTEIILTPDLTALYRIL
                     ESY"
     assembly_gap    1105..1204
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1205..2409)
                     /locus_tag="PKK03_09925"
     CDS             complement(<1205..2409)
                     /locus_tag="PKK03_09925"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNX84758.1"
                     /translation="MGPDGSKKIQAALIAGLLALSLHSFSQESILDRTYRLPGNSIRA
                     SRALGEISRLTGYLFTYDTQIINANRTFTLTGGEMPVRVILDSVAGDTTIHYLIQGRH
                     IILYRETALPPRPDPPADSLPVFLAIGGKIIDAETSEPLPFATIGISHRGKGTVTNFN
                     GDFVLRISEENLYDTLTVSYVGYVNRLLPVRSLPGNVMTITMDRDYIPIPEIIIRAQD
                     PLVIMRKTVSSVASNYGTTPAILTGFYREGVYRKKEPQVYSEAVLKIYKSPYARSLQN
                     DQIKVIRSRKIESLEAKDTLAVRLKAGLSATLSLDGMRHLFDFLDPQSFNSYEYHLTD
                     IVTIDGQTAFVISFKQREWVTDPLMQGDVYINVDDYSLMLAEFEVNPRYVDQTGESFI
                     SRLPRDYSM"
     gene            complement(2428..3411)
                     /locus_tag="PKK03_09930"
     CDS             complement(2428..3411)
                     /locus_tag="PKK03_09930"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNX84759.1"
                     /translation="MKKINETEKWNDREWAGAASWLSGEENGDGENARILASEEADIM
                     KKWNDLKYSGEEPVDVDKAWEKLNGRIEAAEWPVMPLPVKRTPFLSTFARIAAMVIII
                     AGAGWLLFEVAAPQKITLTSDSDQKNIEVLLSDGSTVFLNRNSSLTYPKNFRSGTRKV
                     SLKGEAFFDIARDEEHPFIIDAGKAKIRVLGTSFNVITENAENEVEVYVASGKVMVTS
                     VDGSGSLTLEPEYVGKVSGSNATQERNSNVNYLAWHTGMLSYDGERLGVVFEDLKRAY
                     NIEIKVADPEINDFRLTSPFEQQPQDTIVKLICTTFNLRSVREGSTYILFR"
     gene            complement(3411..3944)
                     /locus_tag="PKK03_09935"
     CDS             complement(3411..3944)
                     /locus_tag="PKK03_09935"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HNX84760.1"
                     /translation="MFSDSEIIGRIRQGDKQEFEKLFRSSYVSLVRYAKTLIRDLDAS
                     EEIVQELFFRLWQDRQTIRIESSLNGYLYRSVHNRALHYLEHQKVVSRHAGEVLAAAD
                     MTSEPVTDAIYYHELQARVARVLERLPERCRLIFKMNRFEGLKYIEIADKLAVSLKTV
                     EADMGKALKEFRKALAE"
     gene            complement(3963..>4698)
                     /locus_tag="PKK03_09940"
     CDS             complement(3963..>4698)
                     /locus_tag="PKK03_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNX84761.1"
                     /translation="LRFDAVRIYKGRKSNDVEKMDTVLFKLQGGPTTTLYLDVVKNPE
                     TFLTREALAQYDLELSNIAMIDDRTNYVINFYQKPSITTPLYQGRLFIDIETYAVAQA
                     EFSFNLENKELAASMFIRKKPMGMQVTPELTSYMVRFREQSGKWYFNHSRAEVKFKVD
                     WKKKLFNTNYTTMSELAITDRTEEDVVKFSNKERMKPTDFFTEEVAAFADPDFWGDYN
                     VIEPDQSIEAAIRKLSRKVKFSDRTE"
     assembly_gap    4699..4798
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4799..5031)
                     /locus_tag="PKK03_09945"
     CDS             complement(<4799..5031)
                     /locus_tag="PKK03_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84762.1"
                     /translation="MKTKVLFTAIASALLLILTAGDMSVSAAPGKSKKERPQQVTTIR
                     GRVIDAETNQPLVFASIAVQGSNVSTVTNLDGE"
     gene            5142..6107
                     /locus_tag="PKK03_09950"
     CDS             5142..6107
                     /locus_tag="PKK03_09950"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HNX84763.1"
                     /translation="MPHGFLNASYSVFSYAAKRYGGIRAIPPLPPAISVELSSVCNLS
                     CPECVTGLGLTRRANSFISYDLAAEIASQSGRRVLSAWLSYQGEPMMHPEFFRIAELF
                     TGMNPVISTNGHFLDPENCRRLADSPLKKIIISWDGATPEVYNIYRKGGDHAVVKEGI
                     RRLAATIKERRSKLEMILQFLVHRGNEHETEAASSFARSVGAGFRVKSMQVLDPAGAG
                     KWAPSDPRKSRFMKGDDGAWRAAESSAKGCLRMWTSAVITTDGDVIPCCYDKNLRHVM
                     GNLGQQTLSGIWRGERYSSFRETVMRSRRQVDICSGCPEGKRLFF"
     gene            6180..8570
                     /locus_tag="PKK03_09955"
     CDS             6180..8570
                     /locus_tag="PKK03_09955"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNX84764.1"
                     /translation="MKILKISLLTIAVILAVVLLAGVIVVPALRKSGLPDLNGEKSLA
                     ALTAEVKVIRDERGVPHIYASNEHDLYYMTGYITAQERLWQMDMVRHATQGRLSELLK
                     RDMFDTDYFLRALGMPKKSELVLEKEDPAILATLQAYADGVNSWIAECGKKLPPEFKI
                     LGHTPEPWTTTDITNIIGYIGWDLASSNLSGEINNYKLGRKLGPEKASELIPDWSMVD
                     EVVFPEFKLDDKLIDKALAAVSSLDKLEELGIVTFSGSNNWAVSGERSETGKPILSND
                     MHMGFNVPGVWMQVHQVIPGKLNVTGVIFPGEPFVIAGHNEKIAWGMTNLSVDDIDLF
                     VETVDSTGNNYLYNGEWLPFREEEHQLKMTDDSSQTRIIRYTHHGPVISEMRGINDEV
                     LTMCWSGYDYSDEIQAVYLLNRAGNWDEFRTALSHFRSISQNFAYADVDGNIGLNAGG
                     GVPVRKGTGLIPRKGDTDEYEWKGYVPFELLPSSFNPEEGFISSANQRTVDESYPFFI
                     SGEFALPYRIMRIREMAAEKQVLGIDDFKRMVTDNHSAYAKMLTPVILKGAEALSTPG
                     ETEKKAIEELRGWDYTMDASLVAPTLFEFIRAELARQIMQDELDELYGSPLGRQYDFY
                     LYSMMKEGPDEWIDNVDTPEKESMETIISRSISAALDTLTARQGGYGENWQWGKVHTF
                     TLEHPMGGVKILNRLLRLNSKSYGAGGSNHTVAPFAFRNNLKAVHGASERHIFNTADW
                     DKSLTVIPTGTSGIPGSPFYLSQTETYVNNGFYSEPFSDAAVEAAKKYEMIFRPGK"
     gene            complement(8620..9183)
                     /gene="frr"
                     /locus_tag="PKK03_09960"
     CDS             complement(8620..9183)
                     /gene="frr"
                     /locus_tag="PKK03_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNX84765.1"
                     /translation="MTEELELIRDEAEDKMEKAVRHLEYELSHLRAGRANPLLLDGIT
                     VDYYGTMTPLAQVSNINTPDAKSILIQPWEKNMLGVIEKAILAANIGMTPMNNGEVIR
                     INVPPLTEERRHQLVKQVKQEGETAKISIRTGRKWFLDELKTLLKEGLPEDEEKTGDR
                     LIQELTDKHINRVDKIMEIKEKEIMTV"
     gene            complement(9228..>9701)
                     /gene="pyrH"
                     /locus_tag="PKK03_09965"
     CDS             complement(9228..>9701)
                     /gene="pyrH"
                     /locus_tag="PKK03_09965"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133821.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNX84766.1"
                     /translation="VINSLALESFLSRAGCNVKVFTSIRMEPVGELYSRDRASAALDC
                     GCVAILAGGTGNPFFTTDTAAALRAVELNCDVLLKGTRVDGVYTADPEKDRSAVRYDS
                     LTFGEAIEKKLRVMDQTAFAMCSENGMPLIVFDMNREGNLRAVAEGNRCGTVITL"
BASE COUNT         2223 a         2365 c         2526 g         2387 t
ORIGIN      
        1 ttccttattt attgtttaat tagcatcgga tgtaaagtaa aaatcaacat gattatgaaa
       61 gcacccggaa ttctgttgtc attactgctt ctggctctca ccgcggtctc ctgtgttacg
      121 ggaaagagat acaacactct cactgacagg aacctgcttc tgatgtcaga gcgtgattcg
      181 ctcaaggcgg aaaacatctg gctgacgatg tcaaacaggg agcttacgac aaagtcgatg
      241 cagcttgcgg gagatgccga gcggctcacc cgggaggttg agaaggcgcg gacagatctt
      301 gcacagtcaa gggatgatgt caaccggctc tccacaaggt acgatgagct gcagagagcc
      361 caggccgagc tggttgccgg gaaagcccag gagactcaga ggctgcttgc agatttgagg
      421 caggcgcaga cgcaacttca gcagagggag gatctgatgc gtgatctcga gatgaacctt
      481 gacaccaaga aggcaaccct ggaggagctc acctatgagc tggaaaagcg caatacacga
      541 ctggctgaac tggagaaaat gcttgacgca cagcagaagg ccgtgaaggc gctgaaggac
      601 aaggtgtctg aagccctgtt cgggtttgaa aacaacgggc tgacggtgac tattaagaac
      661 ggccaggtat acgtttcaat ggatgacaag ctgcttttca ggaccggcag ctatgagatt
      721 gacgccaacg gccgctcggc actgcgcaaa ctcggagcgt tgcttgagaa gaacaccgat
      781 gtgaccatca ccatcgaggg gcatactgat gatgttccgt acagagcagc tccggggcag
      841 cagatcctcg acaactggga cctgagcgtc aagcgggcta caaccgtggt gcgggtgttg
      901 acccaggata cacatatcaa tccaaaacgg tttgtcgcct cagggcgaag cgaatatatc
      961 ccggttgacc cggcgaaaac gcctgatgcc cgccagaaaa acaggaggac ggaaattatt
     1021 cttacccccg acctgactgc actttacagg atacttgaga gttattagag gcctgcctcc
     1081 gcgaacggcc ggaaatccgg gcctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnttcatg gaataatccc tggggagcct gctgatgaat gactcaccgg tctggtccac
     1261 atagcgcgga ttgacctcaa attcagcgag catcaggctg taatcatcaa cattgatata
     1321 gacatcgccc tgcatcagcg ggtctgtgac ccactccctc tgtttgaagg agattacgaa
     1381 tgccgtctgg ccgtcaatgg taactatatc agtcagatgg tattcatatg aattaaatga
     1441 ctgcgggtca agaaaatcaa agaggtgtct catgccgtca agggagaggg tggcactgag
     1501 gcctgccttg agccttacgg cgagtgtatc ctttgcctcg aggctttcaa tcttgcggct
     1561 gcggataacc ttgatctgat cattctgaag agaccgtgcg tatggactct tgtaaatctt
     1621 cagcactgct tcggaataaa cctgcggttc ctttttgcga tagacccctt cacggtagaa
     1681 tccggtgagt atggccgggg tggtgccata gtttgatgcc acggatgaga cggtctttct
     1741 cataatgacc agcggatcct gtgccctgat tatgatctcg ggtatcggga tatagtccct
     1801 gtccatggtg attgtcatta cgtttcccgg gaggctcctt acgggcagca gtctgttgac
     1861 gtagcccaca taggagactg tcagggtgtc ataaaggttc tcctcactta tcctgaggac
     1921 aaaatctccg ttgaaattag taaccgtgcc tttcccgcgg tggctgatgc cgatagtggc
     1981 gaaggggagg ggttcggaag tttcagcgtc aataattttg cctccgattg ccaggaagac
     2041 cgggagagag tcggcgggcg ggtcgggtct gggaggcagt gctgtctcac ggtagaggat
     2101 gatgtgtctc ccctgtatga gataatggat ggtggtatca cctgccacag agtcaaggat
     2161 caccctgaca ggcatttcgc cgcctgtcag ggtgaaagtt ctgttagcat ttattatctg
     2221 ggtatcatag gtaaagagat aaccggtgag cctgcttatc tcacccagcg ccctggatgc
     2281 cctgatgctg tttcccggca gtctgtacgt cctgtcgaga atgctctcct gcgagaatga
     2341 atgcagtgag agcgccagca gtccggcaat cagggccgcc tgtatctttt ttgatccgtc
     2401 tggccccatc agttcctttt gaccctgtca gcggaagagg atgtaggtac ttccctccct
     2461 gacagagcga aggttaaagg tagtgcaaat cagtttaaca atggtatcct gaggctgctg
     2521 ttcaaagggt gaagtgagcc tgaagtcatt aatttcaggg tcagcaacct ttatctcaat
     2581 attatatgca cgtttcaggt cttcaaagac cactcccagg cgctcgccgt cataggaaag
     2641 cataccggta tgccatgcga ggtaattaac gtttgaattg cgttcctgcg tggcattgct
     2701 ccctgacacc ttcccgacgt attcaggttc cagggtcagg cttcctgatc cgtccacgct
     2761 tgtcaccatc actttaccgg aggcgacgta tacttccacc tcgttttcag cattctcggt
     2821 gatcacgttg aacgatgtgc ccagtacccg gatttttgct tttccggcat cgatgatgaa
     2881 cgggtgttct tcatcgcgtg cgatgtcgaa gaaagcttcc cctttaaggg atactttccg
     2941 tgtcccgctc ctgaagttct tcgggtaggt gaggctgctg ttacggttaa ggaagacggt
     3001 gctgccgtca gagaggagca cctctatatt tttctggtca gagtctgaag tcagtgtgat
     3061 tttctgaggg gcagccacct caaagaggag ccatcccgca cctgcaatga tgatcaccat
     3121 tgcagcaatt cttgcaaaag ttgaaaggaa cggtgtcctt ttaacgggca ggggcataac
     3181 aggccattcg gcggcctcga ttctgccatt cagtttttcc catgctttat caacatcaac
     3241 aggttcctct cctgagtatt tcaggtcatt ccattttttc attatatctg cttcttcact
     3301 ggccagtata cgggcatttt caccgtcacc gttctcctct ccggaaagcc atgaggctgc
     3361 accggcccat tcccggtcat tccatttttc tgtttcatta atctttttca tcactctgca
     3421 agtgcttttc tgaactcctt aagtgccttt cccatgtctg cctcaaccgt tttaagcgag
     3481 acagcaagct tgtcagctat ctcaatgtac ttaagacctt cgaagcggtt catcttgaat
     3541 atcagcctgc atctttcggg caatctctcg agcacccttg ccaccctggc ctggagctca
     3601 tgataataga ttgcatctgt caccggctcg gaagtcatgt ctgctgctgc cagcacctcg
     3661 ccggcgtggc ggctgaccac cttctgatgt tccaggtaat gcagtgccct gttgtgtact
     3721 gatctgtaaa gataaccgtt caaagaactt tcaattctga tggtttgcct gtcctgccac
     3781 agcctgaaga agagttcctg tactatctcc tccgaggcgt cgaggtccct tataagggtt
     3841 ttggcatagc gcaccagtga aacataggag gaccggaaca gtttctcaaa ctcctgtttg
     3901 tctccctgcc tgatccttcc tatgatttca ctgtcgctga acatgggcag aaaccccccg
     3961 tcttactctg tcctgtcgct gaatttaacc ttcctgctga gctttcgtat ggctgcctcg
     4021 atgctctggt caggctctat cacgttgtag tcaccccaga aatcagggtc agcaaaagct
     4081 gcaacttcct ccgtgaagaa gtctgttggt ttcatcctct ccttgtttga gaactttacc
     4141 acatcttctt cggtacggtc agttatggca agttctgaca tggttgtata gttggtgtta
     4201 aagagtttct ttttccagtc taccttgaac ttgacctcgg ccctggaatg gttgaaatac
     4261 cactttccac tctgttcacg gaaccggacc atataggagg tcagttcggg tgttacctgc
     4321 attcccatgg gtttcttcct gatgaacatc gatgctgcaa gttccttgtt ttcaaggttg
     4381 aatgagaatt cggcctgtgc tactgcatag gtttcgatgt caataaagag gcgaccctgg
     4441 taaagaggtg tggtaattga aggcttctgg tagaagttga tgacatagtt ggtacggtca
     4501 tctatcatgg cgatattgga gagctccagg tcatactggg caagtgcttc ccgggtgagg
     4561 aacgtttcgg ggttcttcac aacgtcaaga tagagtgtgg tggtagggcc gccctgcagt
     4621 ttaaagagga cggtatccat cttttccacg tcgttgctct ttctgccctt gtaaattctg
     4681 acagcgtcaa acctgaggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa
     4801 ttccccgtca aggttggtga cagttgagac gttgcttccc tgtacggcta tgctcgcaaa
     4861 tacaaggggc tggtttgtct cggcgtcaat aacccttccg cggatggttg taacctgctg
     4921 gggcctctcc ttctttgatt ttcccggcgc agccgaaacc gacatgtcgc ctgcggtcag
     4981 aatgaggagc agtgccgaag caattgctgt aaagaggact tttgttttca tggtctttta
     5041 tttttttgtt cttaatccgg atcattgttc catactaatg accggaattg ccccctctcc
     5101 cctatacatt cacaggaaaa aattgttcat ttgcctttgc catgccacat ggattcctga
     5161 atgcaagtta ctccgttttc tcttacgccg caaagagata tgggggtatt cgtgccattc
     5221 cgccgctgcc gccggcaatc agcgttgagc tgagcagcgt gtgcaacctg tcctgtccgg
     5281 agtgtgtcac cggcctcggc cttaccagga gggcaaacag cttcatcagt tatgaccttg
     5341 ccgcggagat tgcctctcag tcagggcggc gggtgctgtc agcatggctc agttaccagg
     5401 gagaacccat gatgcaccct gaattcttcc gtatagcaga actttttacc ggaatgaacc
     5461 cggtgatttc aaccaacggc catttcctcg atccggagaa ttgcagaagg cttgcagatt
     5521 cgccgctgaa aaaaattatc atctcatggg acggcgccac cccggaggta tacaatatct
     5581 accggaaggg gggagatcat gctgttgtca aagaaggtat ccgcaggctg gccgccacga
     5641 taaaggagag gaggtcaaag ctggaaatga ttctgcagtt cctggttcac aggggtaacg
     5701 agcatgaaac tgaggcagct tcttcctttg caaggtcagt gggtgcaggc ttccgggtaa
     5761 agagcatgca ggtgcttgac ccggccggag ccgggaaatg ggcgccatct gacccgcgga
     5821 agtcacgctt catgaagggc gatgacggcg cctggcgggc tgcagagtca tcagctaaag
     5881 gatgcctcag gatgtggacc tcggcggtaa tcaccaccga cggcgacgtt attccctgct
     5941 gctatgacaa aaacctcagg catgttatgg gaaacctcgg acaacagact ctcagcggga
     6001 tatggagagg agagaggtac agttcattca gggagacggt tatgagaagc cggcgccagg
     6061 ttgacatttg cagtggctgc ccggaaggaa agagactgtt tttttaagat gcgcgccaga
     6121 atgcctattg ccacattgta ttcaattatt aattttaagc aaatcaaacc ggaggtcata
     6181 tgaagattct gaagatttca cttttaacca ttgccgtaat cctggcggtt gtactgctgg
     6241 ccggagtaat agttgttccc gcccttcgca agtcgggcct tccggatctt aacggagaaa
     6301 aaagtttagc ggcccttacg gccgaagtga aggtcatccg cgatgagcgc ggtgtgcctc
     6361 atatttatgc ctcaaacgag catgaccttt attatatgac gggatatatc accgcacagg
     6421 agagactctg gcagatggac atggtaaggc atgccaccca gggcaggctg tcagagctgc
     6481 taaaaaggga tatgtttgat actgactact tcctgagggc actggggatg ccgaagaaat
     6541 cagagcttgt tctggagaag gaagaccccg ccatcctggc cacccttcag gcatacgctg
     6601 acggggtcaa cagctggata gctgaatgcg gcaagaaact tcccccggaa ttcaagatat
     6661 tgggtcatac gcccgagcct tggacaacca cggatataac caatattatc ggctatatag
     6721 gatgggatct ggcatcatca aatctctccg gtgaaatcaa caactacaag ctgggcagaa
     6781 agctgggtcc ggagaaagcc tcggagctca ttcccgactg gagcatggtt gatgaggttg
     6841 tattcccgga gttcaagctg gatgacaagc tgatagacaa agctttggcg gcagtcagct
     6901 cattggataa actggaagag ctgggcattg tcaccttctc gggaagtaat aactgggctg
     6961 tctccggcga acggagcgag acgggcaaac cgattctttc caatgacatg cacatgggat
     7021 tcaacgtccc aggcgtatgg atgcaggtgc atcaggttat ccccggaaaa ctcaatgtca
     7081 ccggagtgat cttcccggga gagcccttcg tcattgccgg gcacaatgaa aaaatagcct
     7141 ggggaatgac caacctgagc gttgatgata ttgacctctt tgttgagacg gttgattcca
     7201 caggcaataa ttatttgtat aatggcgagt ggcttccatt cagggaagag gaacatcagt
     7261 tgaagatgac tgatgactca tcccagacac gcataatcag atacacccat cacggaccgg
     7321 tcatctcgga gatgcggggc attaatgacg aggtactgac aatgtgctgg tcaggctatg
     7381 actacagtga cgagatacag gcagtgtacc tgctgaacag ggccggcaac tgggatgagt
     7441 tccggacagc cctcagccat ttcaggtcaa taagccagaa ttttgcatac gcagatgttg
     7501 atggaaatat aggcctgaac gccggtggcg gagttcccgt caggaagggt acgggactca
     7561 ttccccgcaa gggcgacacc gacgagtatg aatggaaagg ctatgtgccg tttgagctcc
     7621 tcccctcatc attcaatcct gaagagggat ttatctcatc agcaaaccag aggactgtgg
     7681 atgagagcta tccgttcttc atcagcggcg agtttgcatt accctaccgc attatgcgaa
     7741 taagggagat ggctgctgag aagcaggtgc tcggaataga tgatttcaaa agaatggtca
     7801 ctgacaatca ctctgcctat gcaaagatgc ttactccggt gatactgaag ggggcggagg
     7861 cactcagcac tcccggcgaa acggaaaaga aagctattga ggagctgaga ggatgggact
     7921 acacaatgga cgcctcgctc gttgccccga cgctctttga attcatcagg gcggagttgg
     7981 cccgtcagat aatgcaggat gagcttgatg agctttacgg atcacccctg gggaggcagt
     8041 acgattttta cctgtacagc atgatgaagg aagggccgga tgaatggatt gacaatgttg
     8101 acacacccga aaaggagagc atggagacca tcatttcccg gagtataagt gcagcgctcg
     8161 ataccctcac agcccggcag ggcggatacg gggagaactg gcaatggggg aaagtccaca
     8221 ctttcaccct tgagcacccg atgggagggg ttaagatcct gaacagactg ttgcgtctca
     8281 actcaaagag ctacggtgcc ggaggaagca atcacactgt ggctcccttt gctttcagga
     8341 acaacctgaa ggcggtgcac ggcgcatccg aaaggcatat tttcaatacg gctgactggg
     8401 ataagtctct tacagtcatt ccaaccggca cctccggcat accgggcagc ccgttctacc
     8461 tctcgcagac tgaaacctac gtgaataacg gattttacag cgaacccttc tctgacgctg
     8521 cagttgaggc tgcaaagaaa tacgaaatga tcttcaggcc cggtaaatag cccgccggca
     8581 caggcattca ataaaaaaag gggtttcggc cccttttttt tatactgtca ttatctcttt
     8641 ctccttgatc tccatgatct tgtcgaccct gttgatgtgt ttgtcagtca gttcctggat
     8701 gagcctgtct ccggttttct cctcgtcttc aggcagtcct tccttcagca gtgttttaag
     8761 ttcgtcgagg aaccatttac ggccggtccg gatgcttatc ttggcggttt cgccttcctg
     8821 tttcacctgc ttgaccagct gatgccgcct ctcttctgtc aggggaggca catttatccg
     8881 gatgacttcg ccgttgttca tgggggtcat gccgatattg gctgcaagaa tggccttttc
     8941 gatcacaccg agcatattct tttcccatgg ctgtatgaga atgctcttgg cgtcaggggt
     9001 gttgatattg gacacctgtg ccagcggggt catggtaccg taatagtcta cggtaatgcc
     9061 gtcaagcagc agcgggttgg ctctccccgc gcgaaggtgg gagagttcgt actcgaggtg
     9121 cctgacagcc ttttccatct tatcttcggc ctcgtctctg atcagttcca gttcttcagt
     9181 catattgttg tttttattct ggtggaaaca aaagtaggaa aaacttttca gagagtaata
     9241 acggttccgc acctgttacc ctctgcaaca gccctcagat tgccctcccg gttcatgtcg
     9301 aaaacaatca gcggcatgcc gttctcgctg cacattgcga atgccgtctg gtccattacc
     9361 ctgagctttt tttcgatggc ttcgccgaag gtgagggaat catatctcac tgccgaacga
     9421 tccttttccg ggtcagcggt ataaacgccg tcaaccctgg ttcctttcag gaggacgtcg
     9481 cagtttagtt ccacggcacg aagggctgct gccgtgtcag tggtgaagaa gggattaccc
     9541 gttcctccag ccagtatggc cacgcagccg cagtcgagtg cagctgatgc cctgtccctg
     9601 ctgtaaagtt cacccacagg ctccatgcgt attgaggtga agacctttac gttgcagcct
     9661 gcccgtgaca ggaagctctc cagggcgagg ctgttgatca c
//
LOCUS       DAOPXU010000107         9660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_8293_length_9660_cov_10.930245, whole genome shotgun sequence.
ACCESSION   DAOPXU010000107 DAOPXU010000000
VERSION     DAOPXU010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9660
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8293_length_9660_cov_10.930245"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..493)
                     /locus_tag="PKK03_09970"
     CDS             complement(110..493)
                     /locus_tag="PKK03_09970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HNX84767.1"
                     /translation="MALINVITYDAPLPGGHYSQAIVSGNMIFVSGQLPVIPLTGDKL
                     TGNISEQTLQALKNVLSIVKAAGGDITSIARTTIYTTDVKYWEEVNRVYADFFGPTLP
                     ARSIITVAGLHRGCLVEIEAVAFIL"
     gene            complement(513..1178)
                     /locus_tag="PKK03_09975"
     CDS             complement(513..1178)
                     /locus_tag="PKK03_09975"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HNX84768.1"
                     /translation="MSFDYNTQRKRMALPEYGRNVLKMIEHVKTIKDPEERNQAARTI
                     IQIMGNLNPNLREVTDFRHKLWDHIMIIADFDLDVDSPYPAPDRKKLDAKPNKVPYHN
                     GEIKYSHYGRIVPAMIEAAAKMEDGEEKDYLTSLILNQMKKDYLTWNKATVADEIIIR
                     DLTDMSGGRLKVPENFRMPDVRDLLAQPKSKIQGKHQGRPQDKYQGRPGKQQNKKKHK
                     NGY"
     gene            complement(1217..1927)
                     /locus_tag="PKK03_09980"
     CDS             complement(1217..1927)
                     /locus_tag="PKK03_09980"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNX84769.1"
                     /translation="MENSTILLAGTAVTIGFIHTLVGPDHYLPFIVMGEARKWTIRKT
                     MFITFLCGLGHVLSSVIVGFIGIAAGISLSKLEFLEGFRGNIAAWLLIAFGLVYMLIS
                     LRNVYRRKQHVHTHTHADGIAHEHRHDHFSGHSHIHLNDRKNLTPWILFLVFVLGPCE
                     PLIPILMYPAAENNISGVILVSLLFSAVTIATMMAVVLAFRLGISKINLKPLERWVNV
                     IAGATIAVSGLAIQFLGL"
     gene            complement(1974..3107)
                     /locus_tag="PKK03_09985"
     CDS             complement(1974..3107)
                     /locus_tag="PKK03_09985"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNX84770.1"
                     /translation="MRSLKIILLLACLSCAPLILGQEPDSVLTLLFAGDIMGHDGQIA
                     SARNDSSGTYEYDSVFRHIAPFISSVDVATGNLEVTLGGPPYKGYPAFSSPDELAVAC
                     RNAGFDILGTANNHSADRGARGIFRTVKVLDSLEIRHTGTWISPEERDIISPLMICHE
                     YMRIALLAYTYGTNGIVVPPPATVAYIDTIRAAADIRRAEWLGADRTIIFIHWGIEYD
                     TIPSSEQKKTAAALQRAGADIIIGSHPHVVQPVAAETDSAVIRNPVVWSMGNFVSNQR
                     TRRRDGGILIRLDITANSDTAYVSDAGYVLTWVYTPVENGKQKFYILPCAEFEKKPEF
                     FQSSGHYDAMMLYIADARRLLDNHGTGFREMTLTDGKWTGVTR"
     gene            3181..3771
                     /locus_tag="PKK03_09990"
     CDS             3181..3771
                     /locus_tag="PKK03_09990"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HNX84771.1"
                     /translation="MEHLNTDIVNYVLLPLFIFLARIFDVSLGTLRIIFVTKGMRSVA
                     PFVGFFEVLIWLLAISRIMQDLDNWVSYVAYAGGFATGNFVGMYLEERLAIGHEMIRV
                     ITRKDASSLIEDLRNKGYGVTSVKAEGIEGEVAVIYIIARRSMIREVLDEINIFNPRA
                     LYTVESIKYVNKEIFHRSEEERQKSWLAGRMRRIVK"
     gene            complement(3777..4190)
                     /locus_tag="PKK03_09995"
     CDS             complement(3777..4190)
                     /locus_tag="PKK03_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84772.1"
                     /translation="MKPSLILMLLIAALLTSCNLDPGYTMYTDLVIPIEERSVPETGV
                     VNQPVNIYVIAAAYNGCWSNIHFYLTQKDDRQYEIWAAADFESTGQCPDVIISADTIL
                     TFTPARTGDHVISFRMTPEISERDTVVIGTAPGSR"
     gene            complement(4255..5739)
                     /locus_tag="PKK03_10000"
     CDS             complement(4255..5739)
                     /locus_tag="PKK03_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNX84773.1"
                     /translation="MNRILPAAFAILLITSCTRQRETEFFTGRVDFPVQPNLGVKEAM
                     AARLIPHQRQLEWQSLEMTAFIHFTINTFTDREWGTGKESPELFNPSSLDADQWVRVL
                     SEAGMKMVIITAKHHDGFCLWPTKTTPHSVASSPWKDGKGDVIRELKDACEKYNMKFG
                     VYLSPWDRNASCYGDSPAYNRFYMEQLTELLTWYGKVDEVWFDGANGEGPDGRRQEYD
                     WQAYYDLIRKLQPDAVTAIMGQDVRWVGTESGYGRETEWSATVLAPGATPESVAINEA
                     LEVNAMSEDLGSSSLLHRADHLFWYPAEVDVSIRPGWFWHASEDSLVKDVAKLADIYL
                     SSVGRNAVLLLNVPPDNRGLITDQDISSLRGMKAWIDKMYATDLLNGSRPYGRGTSNL
                     TDGDNSTSWNPRRNPVASFVPAIAATFNVAMLQEDISKGQRVEKFIIEALSGAEWDTI
                     ATGTTIGYKRLIRFPEVKADEIKLTIVSSRAAPYISTFALYYTE"
     gene            complement(5749..6156)
                     /locus_tag="PKK03_10005"
     CDS             complement(5749..6156)
                     /locus_tag="PKK03_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84774.1"
                     /translation="MDNILKKLKYGEQARIAVLNAPEEFRGRITGLLPGVQIDSEINA
                     RYLYDFMIAFTPGSAEVDKYGPACIHNLSEDGKLWMVYPKGTSRRYKTNINRDRGWET
                     VTGTGFRPVSQVAIDEDWSALRFRNVKFVKSGK"
     regulatory      6255..6350
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6405..8888
                     /locus_tag="PKK03_10010"
     CDS             6405..8888
                     /locus_tag="PKK03_10010"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX84775.1"
                     /translation="MVRKVSVFMFVFMLLAVIVTGQRRGVTGTGQNQGEVIRPQSHGS
                     IITGTVTDSGGVPMAGASVVLTDAATGIATDNEGRYTLRGLSDGSHNLRISFTGYEPH
                     DTLVVVRGSAVADVTLKEALYVAPEVIVRGSRAGARTPMAHSTISSDELRGRDLTRDM
                     PFLLSLTPSVVETSDAGTGIGYTSLRIRGTDASRINITLDGIPLNDSESQQVFWVDLP
                     DLASSTGSVQVQRGVGTSTNGAGAFGASVNISSLTPPSEAGASADMSYGSFNTSRLSA
                     KAWTGMLGDRFNMMVRASDIHSDGYVDHSTADIKSAMVSGLWSAPSDMTRFNIITGSQ
                     KTGISWWGVPAEILPENRRYNPAGEYVGSDGITSYYEDETDVYTQNHYHLFHTHLFPG
                     RVSLNTGIHLTTGLGYYEEQKSDISPSEYGLSDMFPSDPVIKELDIVQRKWLDNLFYG
                     AVWSLIKQGAGTEWTLGGALNRYDGDHYGTILWIEYPGDINPGYEWYRNRGVKDEVNV
                     YGKVNASLTGSLSGFLDLQLRHIGYRFEGPDDDMKELGGSHRFLFFNPKAGMFWSNGS
                     GSEAFVSAAVAHREPTRADYKDAAGDPSATPGRERLTDFEGGYTFRTSAAALGVNLYY
                     MWYRDQLVPTGRISTTGYPIMTNVPESYRAGVELSGSFRPSPVAALKMNLTLSRSRIR
                     DFRNYYFNYNTDDWSEEYLFSDLGTVDIAYSPRVTGSAELEVNPVERLLLSLTGKYVG
                     KQYFDNTMSADRAIDPYFVSNLSAAYGFRLKKAGGLTLRLMVNNLINTRYENNAYGGM
                     WAEDGDEKTWAYYFPQAGINYTAGISISF"
     gene            8939..9604
                     /gene="pnuC"
                     /locus_tag="PKK03_10015"
     CDS             8939..9604
                     /gene="pnuC"
                     /locus_tag="PKK03_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNX84776.1"
                     /translation="MATNWIAENIVEIFGAVTGIAYVILEIRRNILLWPLGIITSAVY
                     IYVFGREGFYANMGLQVYYLVISIYGWYAWRRTQNNDTGIARRASRIVGPGDPPSSLP
                     TGNIDGAPGTDVRRIDRLTAVWCIFTAVALWAILWFILGRATDSQIPLWDGLIASLSV
                     VATWMLTRKYLEQWYVWIFANAIAVAVYLASGLYPTAVLFFVYFGMAIIGLIKWGKQP
                     LNN"
BASE COUNT         2194 a         2493 c         2561 g         2412 t
ORIGIN      
        1 ttcatcatgc ctctcttcgt gactcttctt ttttaccatg ataaaaacag tttactaagg
       61 gccattaaca aaatgccggc cacaatgata aaactgacaa agtggcaatc taaagtatga
      121 aagccacggc ctcaatctct accaggcatc cgcggtgcag gcctgcgacg gtgattatgg
      181 atctggccgg aagcgtcgga ccaaagaaat cggcgtacac cctgtttacc tcttcccagt
      241 atttaacatc cgtggtatag atggttgtgc gggcaatgga tgtaatgtca cctccggcag
      301 ccttcacaat actgagcaca ttcttaagag cctgaagggt ctgttcgctg atgttaccgg
      361 tcagcttgtc tcctgtcagt ggaatgaccg gcaactggcc cgatacaaag atcatgtttc
      421 cggagacaat tgcctgcgaa tagtgcccgc ccggcaatgg agcgtcatag gtgataacat
      481 tgatgagggc catggtctga aagatctgat tattagtagc cgttcttatg tttcttcttg
      541 ttctgctgct tacccggcct tccctggtat ttgtcctggg gtctgccctg atgcttgccc
      601 tggatcttgc tcttgggctg ggccagcagg tccctgacgt cgggcatcct gaagttctca
      661 ggcaccttca gcctgccgcc tgacatgtca gtcaggtccc tgatgatgat ctcgtcagca
      721 accgttgcct tgttccaggt aagatagtcc ttcttcatct ggttgaggat gagtgatgtg
      781 agataatcct tttcctcgcc gtcttccatc tttgctgctg cctcaatcat ggcaggaacg
      841 attcgcccgt aatgagaata ttttatttcc ccgttatgat aggggacttt gtttggtttt
      901 gcgtcaagtt tcttccggtc aggggcagga taaggcgaat caacgtcaag gtcaaaatca
      961 gcaatgatca tgatgtgatc ccagagcttg tgcctgaaat cagtcacctc cctgaggttg
     1021 ggattcaggt tgcccattat ctgtataatg gtcctggctg cctggttcct ctcctccgga
     1081 tccttgattg tcttgacatg ctcaatcatc ttcaggacat tcctcccgta ttcaggcagt
     1141 gccattcttt ttctttgtgt attgtaatcg aaactcatct gttatatttt gctcaaagct
     1201 actaataaat tcgttctcag agaccaagaa actggatggc aaggcctgag actgcaatgg
     1261 tggctccggc aatcacattg acccaacgct cgaggggttt caggtttatc ttgctgatac
     1321 ccagcctgaa ggccagtacc actgccatca tggtggcaat ggtaactgca gagaaaagca
     1381 gtgagacgag gatcaccccg ctgatgttgt tctctgctgc cgggtacatg agaattggta
     1441 taagcggttc acacgggccc agcacgaaaa caaggaagag aatccacggg gtgaggttct
     1501 tcctgtcatt caggtgtata tgcgagtggc cgctgaagtg gtcatgccgg tgctcatgcg
     1561 cgataccgtc agcgtgggta tgcgtgtgga catgctgttt cctgcggtag acattgcgaa
     1621 ggctgataag catgtacacc agtccgaagg ctatcagcag ccaggctgca atattccccc
     1681 tgaatccttc gaggaattcc agttttgaga gggagatccc tgcagcaatg cctatgaatc
     1741 ccacgattac agaactcagg acatgtccga ggccgcagag aaaggtgatg aacatcgttt
     1801 tcctgatggt ccatttccgc gcctcgccca taacaatgaa cggcagatag tgatcgggtc
     1861 ccaccagcgt gtgaatgaag ccgattgtta ctgcagtgcc tgccagaagt attgttgagt
     1921 tctccattac cggattttaa cagtgtgtaa agataatgat attgacggtc cggctacctt
     1981 gtaacccctg tccactttcc atcggtcagg gtcatctccc tgaatcccgt tccatggttg
     2041 tcaagcagcc gccttgcgtc tgcaatgtag agcatcattg catcataatg cccggaagac
     2101 tgaaagaatt ccggtttctt ttcaaattca gcgcagggca ggatgtaaaa cttctgtttg
     2161 ccgttttcca ccggggtgta gacccaggtg agaacgtatc cggcgtcaga aacataggcg
     2221 gtgtcactgt ttgcggtgat gtccagcctg atcaatatcc cgccgtccct tctccttgtc
     2281 ctctggtttg atacgaagtt gcccatggac catactacag gattcctgat tacagcagag
     2341 tctgtttcgg ccgccaccgg ctgcaccaca tgcgggtggg agccgatgat gatatcagcg
     2401 cccgccctct gcagcgccgc cgccgtcttc ttctgttcgg atgaaggaat ggtatcatat
     2461 tcaatgcccc agtgaatgaa tattattgtc cggtctgccc ccagccattc agccctccgg
     2521 atatcagcgg cagcacgtat ggtgtcaata tatgcgaccg tagccggtgg cggcaccact
     2581 atcccgtttg tgccgtaggt gtaagccagc agggctattc tcatatattc atggcatatc
     2641 atcaggggag agatgatatc acgttcttcc gggctgatcc atgtgccggt atgccttatt
     2701 tccaggctgt caagtacctt aacggtgcga aagattcccc gagcccccct gtcggctgaa
     2761 tgattgttcg cggtccccag gatatcaaaa ccggcattgc ggcatgccac tgccagctca
     2821 tcgggcgaac tgaaggccgg gtagcccttg tacggaggcc ctcccaacgt tacttcgaga
     2881 ttgcctgtgg caacatcgac ggatgagatg aagggggcaa tatgcctgaa gacactgtca
     2941 tattcgtatg tgcctgaaga gtcattgcgc gctgaggcaa tctgcccgtc atgccccata
     3001 atgtcaccgg cgaagaggag ggtaagaacc gaatccggtt cctgccccag gatgagaggg
     3061 gcacagctca gacatgccag cagtaaaatg attttcaacg atctcatcgc cacatggttt
     3121 tggggtatcg gtttcaaaaa tagtcatatc ttagcaagtt caaaaatgtt tgttgctgcc
     3181 atggaacatc ttaatacaga tattgttaat tatgtcctgc tcccgctctt cattttcctg
     3241 gccaggattt ttgacgtttc cctcggcact ctaaggatca tcttcgtcac caaggggatg
     3301 aggagtgtag ctccgttcgt gggctttttc gaggtgctta tctggcttct ggccatcagc
     3361 aggatcatgc aggatcttga taactgggtc agttatgttg cttatgccgg tggattcgcc
     3421 accggtaatt tcgtggggat gtacctcgag gagcgtcttg ccataggtca tgagatgatc
     3481 agggttatta cacgcaagga tgccagcagc cttattgagg acctcaggaa caagggttac
     3541 ggcgtgacat cggtgaaagc cgaaggaata gaaggggagg ttgctgtaat ctatattatt
     3601 gcacgcagga gcatgatcag ggaggtgctt gacgaaatca acattttcaa cccgcgtgca
     3661 ctctatacgg tggaatcaat caagtacgtc aacaaggaga tctttcacag gtctgaggag
     3721 gagcgtcaga aaagctggct tgccggcagg atgagacgaa ttgtgaagta gcccggttac
     3781 ctgctccccg gggcagtgcc tattaccacg gtgtctcttt cacttatctc cggagtcatc
     3841 cggaatgaaa tgacatgatc ccccgtccgt gcaggggtga aggtgagaat tgtatctgcc
     3901 gagattatca cgtcagggca ttgcccggtt gattcgaagt cggctgctgc ccagatctcg
     3961 tattgacggt catccttctg tgtcaggtaa aaatggatgt ttgaccagca tccgttgtat
     4021 gcagcggcaa tgacataaat gttaaccggc tggttgacta cacccgtttc cggcacactg
     4081 cgctcctcga tgggaatcac cagatcagtg tacatagtgt aaccgggatc aagattgcat
     4141 gatgtcagaa gggctgcgat cagaagcatt aaaataagtg aaggtttcat gtttgagttt
     4201 attattctcc cgtttgcttt tacagccctt caaagccaca ataaccatac caaatcattc
     4261 ggtataataa agtgcgaagg tgcttatata aggggctgcc cgtgatgaga ctatggtgag
     4321 ttttatctcg tctgccttta cctctggaaa ccgtatcagc cttttataac cgatagttgt
     4381 gcctgtagcg atggtatccc actcagcgcc ggagagagcc tcgattatga atttctctac
     4441 ccgctgcccc ttgctgatgt cttcctgcag cattgccaca ttgaatgttg cggctatggc
     4501 cggaacgaat gaggccacgg ggttgcggcg cggattccac gaggtactgt tgtctccgtc
     4561 agtaaggttc gatgttcccc gtccgtatgg cctggaaccg ttgagcaggt cagtggcata
     4621 catcttgtct atccaggctt tcatcccccg gaggctgctg atatcctgat cagttatgag
     4681 cccgcggttg tcaggcggta catttagcag cagaacggca ttgcgtccca cagaactcag
     4741 gtagatgtca gccagctttg caacgtcctt taccagtgaa tcttcggaag catgccagaa
     4801 ccaccccggc cttattgaca cgtcaacctc cgccggatac cagaagagat gatctgccct
     4861 atgaaggagg ctgctgcttc caagatcctc tgacatggca tttacctcca gtgcctcgtt
     4921 gatggcgaca ctctccgggg ttgccccggg tgccagtacc gtggcgctcc attcggtttc
     4981 gcggccatag ccgctttctg ttccaaccca cctcacatcc tgacccatga tggcggtgac
     5041 ggcatcgggc tgaagtttcc tgataaggtc atagtatgcc tgccagtcat attcctgtcg
     5101 ccggccgtca ggcccttcgc cgttggcgcc gtcaaaccac acctcatcaa ccttgccgta
     5161 ccatgtcagc agctcggtaa gctgctccat gtaaaagcgg ttgtaggccg gcgagtcacc
     5221 atagcatgac gcattcctgt cccagggaga gaggtagacg ccgaacttca tattgtactt
     5281 ttcacatgca tccttgagct ccctgatgac gtcacccttg ccatccttcc atgggctgga
     5341 ggcaacggag tgaggggttg tcttcgtggg ccagagacag aacccgtcat gatgcttggc
     5401 agtaattata accatcttca tgccggcctc gctgagtacc ctgacccatt ggtccgcgtc
     5461 aagcgaggaa ggattgaaga gctccgggga ttccttgccg gtaccccatt ccctgtcagt
     5521 gaaagtgttt atagtgaagt gtatgaatgc cgtcatttcc agactctgcc actcaagctg
     5581 ccgctggtgg gggataagcc gcgcagccat cgcctccttc accccgagat tcggctggac
     5641 ggggaagtca accctcccgg tgaaaaactc agtctcacgc tgtcgtgtac aggatgtgat
     5701 cagtaagatt gcgaaggcag ccggcagtat cctgttcatc agaaaaaact atttgccgga
     5761 tttgacgaat ttgacattcc ggaacctgag ggctgaccag tcttcgtcaa tggctacctg
     5821 gctcacaggc ctgaatccgg taccggtaac cgtttcccat ccccggtcac ggttgatatt
     5881 ggttttgtac ctgcgggaag tgcctttcgg gtagaccatc cacagcttgc cgtcctcact
     5941 aaggttatgg atacacgccg ggccgtactt gtcaacttcg gcagagccgg gagtgaaggc
     6001 aatcatgaaa tcatacaggt agcgggcatt gatctcagaa tcaatctgta cgcccggaag
     6061 aagcccggtg atgcggccac ggaactcctc gggagcgtta agcaccgcaa tccgtgcctg
     6121 ttccccatac ttcagttttt tcagaatatt gtccatttct ttgtaattct tcccgggaca
     6181 aatatacaaa taacagagaa ttgcaattgc cgggatccgc cggtggtaat ttcacattta
     6241 tttggtacct ttgcggtgtc ttttcggggt gccttactta acaggctgag atcataccct
     6301 taaaacctga tccggataat gccggcgtag ggaaagagag taatcatttc cggcttgtct
     6361 gaacaccctt gattaagacg atgtttattt aaaaaactgg agaaatggtg agaaaagttt
     6421 cggttttcat gtttgtattt atgcttctgg cagtaattgt taccggtcag aggaggggcg
     6481 ttacgggtac cgggcagaac cagggagagg tgatccggcc gcaaagccac gggagcataa
     6541 tcaccggcac ggtcactgac agtggcggtg tgcctatggc aggagcttct gtggtactca
     6601 ctgatgccgc tacgggtatt gccacggaca atgagggaag gtataccctt cggggcctga
     6661 gcgacggttc ccataacctg cggatcagct ttaccggtta cgaaccacat gataccctgg
     6721 tagttgtgag aggctcggct gtggctgatg tcacacttaa ggaggctctt tatgttgccc
     6781 cggaggttat tgtcaggggc agtcgcgccg gggcgcgaac acctatggca cacagtacca
     6841 tcagctctga tgaacttcgc gggcgggacc tgaccaggga catgccgttc ctgctctcgc
     6901 ttacaccatc agtggtggag acctccgacg ccgggacggg cattggctat acctccctgc
     6961 gcatacgagg aacagatgcc agccgcatca acatcacact tgacggtatc ccgctcaacg
     7021 actccgagtc gcagcaggta ttctgggtcg atctgcctga cctggcatca tcgacaggca
     7081 gcgtacaggt gcagcggggc gtgggtacat ccacgaatgg cgccggagct tttggcgcct
     7141 cggtgaacat aagcagcctg accccgccct ctgaagcagg cgcctcggct gatatgtcat
     7201 acggctcttt caacacctcg cgcctttcgg cgaaggcatg gacaggaatg ctgggcgaca
     7261 gattcaacat gatggttcgc gcatcagaca tccacagcga cggctatgtt gatcacagca
     7321 ctgctgacat taagtcggcg atggtatccg gcctgtggtc ggcaccgtca gacatgaccc
     7381 gcttcaacat tattaccggc agccagaaaa cggggatcag ctggtggggg gttccggctg
     7441 aaatccttcc tgaaaacaga aggtacaacc cggcaggaga gtatgttggc tctgacggta
     7501 tcacctctta ttatgaggat gaaacggatg tctataccca gaatcattac cacctcttcc
     7561 atacacatct cttcccgggc agggtgagcc tgaacaccgg gatacacctt accacgggac
     7621 tgggttacta tgaggaacag aagagcgata tcagcccgtc agaatacggt ctgtcggaca
     7681 tgttcccgtc ggacccggtt atcaaggaac tggatatcgt acagcggaaa tggctcgata
     7741 acctcttcta cggggcggta tggtcgctta ttaagcaggg cgccggtact gagtggacac
     7801 tcggtggagc gctgaacagg tatgacggcg accattacgg aacaattctc tggatagagt
     7861 acccgggtga catcaatccc ggctatgagt ggtacaggaa cagaggggta aaggatgagg
     7921 ttaacgttta cggcaaggtt aatgccagcc tgacaggaag cctgagcggg ttccttgacc
     7981 tgcagctgag gcatatcggt taccgtttcg agggaccgga tgacgatatg aaggagcttg
     8041 gcgggagcca ccgcttcctc ttcttcaatc ccaaggcagg catgttctgg agcaacggaa
     8101 gcgggagcga agcctttgtc tcggccgccg tggctcatcg cgaacctacc cgcgctgact
     8161 ataaggatgc cgccggcgac ccttcagcta ccccgggccg cgagaggctg accgacttcg
     8221 agggagggta taccttcagg acctccgcgg cagccctggg tgtcaacctg tattacatgt
     8281 ggtaccgcga ccagctggtc cctacaggca ggataagcac cacgggttat cccattatga
     8341 caaatgtgcc tgagagctac agggcagggg tggagctgtc gggaagcttc cgcccgtcac
     8401 ccgtggcggc gctgaagatg aatctgaccc tcagccggag caggatcaga gacttccgga
     8461 actattactt caattacaac actgacgact ggagcgagga gtatctcttc agcgatctcg
     8521 gaacggttga tattgcctat tctcctcgcg taaccggctc cgccgagctg gaggtcaatc
     8581 ctgtagaaag gctcttgctc agccttacgg gcaaatatgt gggaaaacag tattttgaca
     8641 acaccatgag tgctgaccgg gccattgacc cctattttgt aagcaatctt tctgctgctt
     8701 atggtttcag gttgaagaag gccggcgggc tgacgctgcg gttaatggtc aacaacctga
     8761 tcaacacgcg ctatgagaac aatgcttacg gcggcatgtg ggccgaggac ggtgacgaga
     8821 agacgtgggc atattacttt ccgcaggccg ggataaacta cacggcaggt atttctatat
     8881 ctttctgatc cgatggcaac tgaacttatg acaggtttct attctttccg cttactcaat
     8941 ggcaacaaac tggatagctg aaaatattgt tgagatcttc ggggcggtca ccggtattgc
     9001 ctatgtgatc ctggagatca ggcgcaacat acttctctgg ccgctgggga taatcacctc
     9061 ggcagtgtat atatatgttt ttggccggga gggtttctat gccaacatgg gacttcaggt
     9121 ttattacctt gtcataagca tttacggctg gtacgcctgg cgccggactc aaaacaatga
     9181 taccgggata gctcgtagag cttcaagaat tgtaggtccg ggcgatcccc catcatccct
     9241 ccccaccgga aacattgacg gggcccctgg caccgatgtc cgccggattg acaggctaac
     9301 agctgtatgg tgcatattta ctgccgttgc actgtgggca attctttggt tcattcttgg
     9361 ccgcgcaact gactcacaga tacccttgtg ggatggtctt atagcttcac tttcagtagt
     9421 ggcaacatgg atgctcacca ggaaatacct cgagcaatgg tatgtatgga tttttgccaa
     9481 cgccattgcc gttgccgttt acctcgcctc cggcctctat cccacagcgg tgctgttttt
     9541 tgtatatttc ggtatggcaa ttatcggact aataaaatgg gggaagcagc ccctgaataa
     9601 ctgatcgcac aaccctaatc ccctgacctt cagaccttca gacctcagac cttcagacct
//
LOCUS       DAOPXU010000108         9561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_8402_length_9561_cov_10.851357, whole genome shotgun sequence.
ACCESSION   DAOPXU010000108 DAOPXU010000000
VERSION     DAOPXU010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9561
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8402_length_9561_cov_10.851357"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PKK03_10020"
     CDS             complement(<1..753)
                     /locus_tag="PKK03_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNX84777.1"
                     /translation="MKKIVHHSLLTFCLAFVLNTLCNAQEHNLLSDSIYSGILKEERS
                     LKVLLPDTWKPGSAEKYEVIYITDGEWAMDPFSFVYKWAQGENFVPPAIIVSVPNRYI
                     NSANQRDRDFLPVHVPQPAISGGAGNFLSFFKDELIPYIDKTYPSNGTSSLYGHSYGG
                     LFVLYALLSEPQLFESYYATDPPFRFNDDYLIKMAAQKLENLPPDKILWLAGNELSYK
                     GQGIDRLDSVLRAKAPASLHWKMVTYPNETHNS"
     gene            970..1374
                     /locus_tag="PKK03_10025"
     CDS             970..1374
                     /locus_tag="PKK03_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HNX84778.1"
                     /translation="MKKGNKLRDKESVLRVEAEYDQAWQAGDVEGIVTRLTKDAVVIS
                     PRGDVACGHQEIRDLFIEFLGGPAKGSTHTSRIIRVNFVTDDVAVLDGEALIEGGEFA
                     NVSSLAHHMFTDILVRRGDVWLIAQIRAYSRY"
     gene            1461..2234
                     /locus_tag="PKK03_10030"
     CDS             1461..2234
                     /locus_tag="PKK03_10030"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNX84779.1"
                     /translation="METSGTIKIKPMGIIPSILFFGIPSLIFSFSIYYVMQKLHQNGT
                     NDFVNYIVSMTSPLVLMLVAAIIAYRIEGNSFGWTAFRTRFRLKRLTRKDWIYALVLF
                     VVMAIIYETLSFTPKWLIEFKLFSPPEFLIPAVDPRLEQNIFLDSFLGIPLKGQWWIA
                     ITYFIVLIFNIIGEEFWWRGYILPRQELAFGKWTWVIHGTLWGLFHIFWKWNLIILLP
                     SCLALSYVIYKRKNTWIGIITHMAFNSVPLIGLIVGIIG"
     gene            2309..3142
                     /locus_tag="PKK03_10035"
     CDS             2309..3142
                     /locus_tag="PKK03_10035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNX84780.1"
                     /translation="MISNYKKPGLFYLLSTILPWTFWFTAGYISHLPSNAERNMNIAI
                     MLALVGLVSPMIVAFLLLNRNPDLRRDFYRRFFNFRDINPWYIFLTCFIMLASILVAM
                     GISLLFGYSPGQFVITGHFSFTSGVLPVWFLLIIAPVFEELAWHSYGTDCLRNKFSLF
                     YTSLIFGAYWAVWHAPLSTIKDYYQANLVESGWIYSLNFMVSIIPYVLIMNWLYYKTR
                     RNILLPVIFHITAVFFNEIFATHPDSKIIQTILLLILAGYLIIRDRKFFFSKDISLIY
                     T"
     gene            3285..4550
                     /locus_tag="PKK03_10040"
     CDS             3285..4550
                     /locus_tag="PKK03_10040"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNX84781.1"
                     /translation="MKKQIRVLLAFMVSGVVLTSCTTREVSCTLKGRIHNRNSDTLIL
                     KRATDDPRFNQVLIPVRDSAFECTIQISHPEAYELIFKEELERGSWRPVLFIAEKGEI
                     SFELYSNPDENKITGRNLNSVLAEYNKEFLGMFRPKADPLNDSIDALFQKNEYFSAEM
                     KLLQSELANAGDNESRMALREKMEDLRSTGNDLSPRAKDLTRQGDSLRAEATRWRYKY
                     IEENPSIVSYYFLLNDLRYLESSQASIKEIKSLYPVFAGRYPKHAYTEIMRSMLEGYD
                     AIKVGGSFIDFSLPDLDGTVHRLSDIIGGKYALIDLWASWCGPCIATSRSMIPVYEEF
                     RDRGFTVCGVAAEIDNTDQMRARIEKEKFPWINLVELDHKNHIWDKYGVSNSGGGTFL
                     MDNEGTILAINPDAEKVREILSEKLKQTF"
     gene            4647..5216
                     /locus_tag="PKK03_10045"
     CDS             4647..5216
                     /locus_tag="PKK03_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNX84782.1"
                     /translation="MTELEKYIHTYFSVSKDDLTSISAFFKPIILKKGEYYLRTGRQS
                     DRLGFVQSGIIREFVIINDREVTKWISTKGYFVVDLSSFMFHLPARWNIQALTDCEIY
                     VIDSMDYQKIGQVVPGWTELEKLFIAKCFTVLEDRIVTHLSMTAEERYNQLFSINKEL
                     FNQVPLQYLASMLGMTPETLSRLRKKMIG"
     gene            5367..5819
                     /locus_tag="PKK03_10050"
     CDS             5367..5819
                     /locus_tag="PKK03_10050"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNX84783.1"
                     /translation="MKRINSSAPVKCSKTVIISANSEKVWAVLTDISNWPAWQTDISK
                     SFLHGDLKPGRNFNWTSGGANILSTIHTVEPFSYFGWTGKAVGLFAVHNWILMETSGQ
                     TIVTVEESMEGFLAVLLRKSLNRNLEKGMHNWLALLKQACEKKQAIPA"
     gene            5874..7328
                     /locus_tag="PKK03_10055"
     CDS             5874..7328
                     /locus_tag="PKK03_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84784.1"
                     /translation="MKRYTLNSVILLLTLTLLISKGHSQVIVKDTLPPSFEWSLHLFD
                     FPYMVDAAKTEAIRAGGGDLPQSVTPGAGDYGNFYRNLSMEQTTEMARNLHGSLYYGH
                     NVIWNVVIEPNSTKRYILNRVVANIAALGTDYLAIKLPYGFAYQHEEFHRAVMSSRHI
                     YSYDEVWKFGKGLDIAVTNVKDEDLVYLKANHPADQVRLSAAGVEGEYAFLLRMREDN
                     FFRQTGYPFLGISLLGTIHAVSYVNLPFTERFNSITDSILAHDRDNILARDFTGWDFS
                     AWVYDLFTPEEPYEDRGTWPGGLGIKRPVKESDLTAEMKDFLRETGNMQYLNFISPFM
                     IGINRIQLKPGYFFNFALRSIPTSFGYYAGGDFFLDFNDRQVLLNLGINRSKSLILPS
                     VDVTYYNIIRREDSRFSANVNLSGWLQPKDQLFAADKASPGMAVGVQPRFDIGERFTI
                     MADLSYKTKGWVFGNPYLDEKLTFRLGFGLNTKN"
     gene            7424..8449
                     /locus_tag="PKK03_10060"
     CDS             7424..8449
                     /locus_tag="PKK03_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNX84785.1"
                     /translation="MIKNSFRWIASHQVTSFFIITFLITWGLGFSYIAVMREKYLFLP
                     LMFLATCGPAIAGIIVASISDSDRQAARKRFSWLALLICWIVVTMAFITNQFFVNKGQ
                     VSTPLVILISISSLPVAFIVNLAYTRFPVIRGFKLKSTVSGGSHTWIIIAFLLFPCLT
                     IISILVSRILGHETPSLSGLPATGFGLAGLFAIKFLSQFFYFNGTGEEVGWRGFALRR
                     MQARSSPLVAGLILALFWVPWHMFLWTGEGRDVMKVSFWASSYLLHIPSAIILCWCYN
                     RSRGSVLAAGIAHASANTIVALIQPLDQNIMIIVFFVFTAAIVILDRMWKPLPEEHPA
                     VQRIRES"
     gene            8480..>9561
                     /locus_tag="PKK03_10065"
     CDS             8480..>9561
                     /locus_tag="PKK03_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNX84786.1"
                     /translation="MNLRRACMHTRSQPKIQYMKGVFLFILLVAVISCNTDKKFTITG
                     FTSRLPDSTAIYLRKASASQICDSTMLSGNSFRFQGAVDSIEQYVVHTRDFQDYISLW
                     IDNSEITIDASKYKLRTAKVSGSEFQELNSSYLELENNLKAKKDSIDMIIRITNKSDT
                     VRLESLYRIRDSLQTVKQNAILDFMVSNPEFYLNPYYLTFLMYNQPLQVTEKMFNALS
                     VPSREGHWGKAINTFLNESVELKTGDQAIDFSLPDINGNQVSLNSYRGKYVLLEFWAS
                     WCGPCRLENPNLLDAYRKYRDQGFEILGVSLDEQKTDWESAIREDTIGWTTVSDLRGM
                     LGDVPVRYRVNGIPQNYLIDPQGKII"
BASE COUNT         2666 a         2033 c         2247 g         2615 t
ORIGIN      
        1 gctgttatga gtctcattgg ggtatgtgac cattttccag tgcagacttg ccggtgcctt
       61 agctctcaga accgaatcca ggcgatctat cccctgacct ttgtatgata attcattgcc
      121 agcaagccat aaaatcttat cgggcgggag attttccagt ttttgggctg ccattttaat
      181 cagataatca tcattaaacc gaaaaggagg gtcagttgca taataagact caaataattg
      241 aggttcagaa agcaaggcat acaaaacaaa caaacctccg tatgaatgcc cataaaggga
      301 actggttcca ttagaagggt atgtcttgtc aatatacggg atcagttcgt ctttaaaaaa
      361 agacaggaaa ttacctgctc cgcctgatat agctggttga ggaacatgta ccggcaaaaa
      421 atccctgtca cgctggttag cagaattgat atatctgttc ggaacagaaa ctatgatggc
      481 aggcggaaca aaattttctc cctgagccca tttgtataca aaggaaaacg gatccatagc
      541 ccactctccg tcagttatat agataacttc atatttttct gctgatccgg gcttccaggt
      601 gtcaggcaag agtactttga gagatctttc ctctttcaaa ataccggaat aaattgaatc
      661 actcagcaga ttgtgttcct gcgcattaca taaagtgttc aggacgaaag ccagacagaa
      721 ggtcagtagc gagtggtgaa cgattttttt catgatgtca ggtttattgg ttgaaggatg
      781 aataaaagac cgattaaatt tacaaatgct gcctgaaaaa aattagattt aaggaagtct
      841 ttaacataat ttgtcaatga aagcaatccg tttacttacc agggccatgc tttccgggca
      901 aatacctcag acctgacggg tttgacattt gaatccatac caacaaggca aaataatttt
      961 acaatagaaa tgaaaaaagg aaataaactg cgagacaagg aatcagttct cagggttgag
     1021 gctgagtatg accaggcctg gcaggcggga gatgttgagg gaatagtcac ccgccttaca
     1081 aaagacgcgg tggtgataag ccccaggggt gatgtggcct gtggtcacca ggagatccgt
     1141 gacctgttca ttgagttttt aggtggtccc gccaaaggtt cgacacacac aagccgtatt
     1201 atccgggtaa attttgtgac ggacgatgtg gcagttctcg atggggaggc tcttattgag
     1261 ggcggggaat ttgctaatgt atcctccctg gcacatcata tgttcaccga tatcctggtc
     1321 cgcaggggag atgtctggct aattgctcag atccgtgcat attcaagata ttaggttcaa
     1381 aaaaagtata ccattttgaa cctcattttt ggggtaaata ataggcaaac taacagcagg
     1441 ctaaacagaa cagaccattt atggaaactt caggtactat taaaattaaa ccgatgggga
     1501 taatcccctc tattttgttt tttgggattc catctctgat ttttagtttc agcatttact
     1561 atgtcatgca aaagctccac cagaatggaa ccaacgactt tgtcaactac attgtatcaa
     1621 tgacttcccc gttggtatta atgcttgtgg ctgcaataat tgcgtacaga attgagggga
     1681 atagctttgg ctggactgcg ttccggacaa gattccggct taagagattg accaggaaag
     1741 actggatcta tgcccttgtt ctttttgttg tcatggccat aatttatgag acactctcat
     1801 tcacccccaa atggcttatt gagttcaagc ttttttctcc tcctgaattt ctgatccccg
     1861 cggttgaccc gagacttgag cagaacattt tcctggactc tttcctggga atacccttaa
     1921 aaggacaatg gtggattgcg atcacatatt ttatagtctt aatattcaat attatcggag
     1981 aagaattctg gtggcgggga tatattctgc cccggcagga gctggccttc gggaaatgga
     2041 cctgggtaat tcatggaaca ctctggggat tgtttcatat tttctggaaa tggaacctga
     2101 taatcctttt gccgtcatgc ctggcccttt cctatgtaat ttataaacga aaaaatacct
     2161 ggataggaat cataactcat atggctttta acagtgtgcc tttaatcgga ctcatagtcg
     2221 gaataatagg ataaagagca ccttttctga acgggcaatc agaaatcacg aatcttaact
     2281 cgggtaccaa atcataactc atgataatat gatcagcaat tacaaaaaac cgggcctttt
     2341 ctatctttta tctacaatcc tgccatggac cttctggttc actgccggtt acataagcca
     2401 cctgccatcc aatgcagaac ggaatatgaa tattgcaata atgcttgctc tggttggatt
     2461 ggtgagccca atgatagtag ccttcctgct cctgaatcgc aatccggatc tgcgcaggga
     2521 tttttatcgc aggttcttta atttcagaga tattaatccc tggtacatct tccttacatg
     2581 cttcatcatg ctggcaagca tccttgtcgc aatgggaatt tctttactat ttggatacag
     2641 tccgggtcag ttcgtaataa ccgggcattt ttcctttacc tcaggagttc ttccagtttg
     2701 gtttttgctt atcatagccc cggtttttga agagctcgca tggcattcgt atggcactga
     2761 ttgtcttaga aataaattca gtctgtttta tacttccttg atttttggag cttactgggc
     2821 tgtttggcat gctccgttgt caacgattaa ggattactac caggcaaacc ttgtcgagtc
     2881 tggttggatt tatagcctga acttcatggt cagtatcatc ccatatgtct tgatcatgaa
     2941 ctggttatat tataagacca gaaggaatat tttgcttccg gtcattttcc acataacagc
     3001 tgttttcttt aatgagattt ttgctactca ccctgacagt aaaattatcc agactattct
     3061 cctgttaatc ctggcaggat acctgataat aagagaccgg aagttcttct tttctaaaga
     3121 cataagttta atctatacat aaatcaccaa ctctgacaaa aggtgctgca tgaccgcaga
     3181 gacctttacc cggcttgttg ccattggccc tggtaaaccc gttatgaata tgctatatgg
     3241 cttgattata attgattaat atacaattaa ataattatat ttcgatgaaa aaacaaatca
     3301 gagttttact cgcatttatg gtctccggag ttgtccttac cagctgtaca accagggaag
     3361 tatcctgcac tctaaagggc agaattcata acagaaacag tgataccctt atcctgaaaa
     3421 gggcaactga tgatccccgg tttaaccagg tgttaatacc ggtcagggac agtgctttcg
     3481 aatgcaccat acagatctca catcctgaag cgtatgagtt gatattcaag gaggaattgg
     3541 aaaggggttc ctggcgaccg gtattgttta tagctgagaa gggagagata agcttcgagc
     3601 tgtacagcaa tcctgatgaa aataagatta ccggcaggaa cctgaacagc gttctggcag
     3661 agtataacaa agagtttctt ggtatgttcc ggcctaaggc ggatccgtta aatgacagca
     3721 tcgatgcatt gtttcagaag aatgagtatt tcagtgcgga aatgaaattg ctgcagtcgg
     3781 aactggcaaa tgctggtgac aatgagagca ggatggcgct tcgggaaaaa atggaagatc
     3841 tgcgttctac gggcaatgat ttgagccccc gggccaagga cctaacccgt cagggcgaca
     3901 gcttaagggc agaagccaca aggtggagat acaaatacat cgaggaaaat ccgtccatcg
     3961 tctcatacta ctttctgttg aatgatctca ggtaccttga atcaagccag gcaagcatta
     4021 aggagataaa aagcctttat cccgtgtttg ccggaaggta cccgaagcat gcctacaccg
     4081 agattatgag gagcatgctt gaaggctatg atgctataaa agttggcggc agctttatag
     4141 atttctcact gccagacctg gacggaaccg ttcacaggct ctctgacatc ataggcggca
     4201 aatatgctct tattgatctg tgggcttcgt ggtgcggacc atgtatagca acaagccgca
     4261 gcatgatccc tgtttatgaa gaattcaggg acagaggatt tactgtttgt ggtgtggctg
     4321 ccgagattga taacactgat cagatgagag ctcgcataga gaaggagaag tttccgtgga
     4381 taaatctcgt agagcttgac cataagaatc atatctggga taagtacgga gtgtcaaatt
     4441 cgggtggcgg aacattcctg atggataatg aagggacaat tcttgcaatt aatcccgatg
     4501 ctgaaaaggt gagagaaatc ctgtcggaaa agttaaaaca gactttttag cgaaccgccg
     4561 gttgtactga attgtttact gtacagcaaa gatcacaata cctgctccgg tcacctgaaa
     4621 ggtgtaacag gctgattact gcggaaatga cagaacttga aaaatacata cacacctatt
     4681 tcagtgtcag caaggatgac ctgaccagta tcagcgcctt ttttaaacct attatcctca
     4741 aaaagggcga atattatctc aggaccgggc gacagtccga caggcttggt tttgtgcagt
     4801 cggggattat cagggaattt gtaatcatca atgacaggga agtcacaaag tggatctcaa
     4861 caaagggata ttttgtggta gacctttcaa gtttcatgtt tcaccttccg gcccgctgga
     4921 atattcaggc attgacagac tgcgagatct acgttattga cagtatggac tatcagaaaa
     4981 tcgggcaggt agttcccggc tggaccgaac tcgaaaaact gttcatagca aagtgtttta
     5041 cagtactcga agacagaatt gtaacccatc tctcaatgac tgctgaagag agatacaatc
     5101 agctcttcag tataaataaa gagttgttca accaggtgcc cttgcaatac ctggcctcga
     5161 tgcttggcat gacaccggaa acattgagca ggctgagaaa aaaaatgatt ggctgaagtt
     5221 cttgattttt gtcaagagca cgggctggct ttgtaaggat ctttgtgtcg tcggcagtaa
     5281 tgacgacagt tcttacgaaa tcagcttttt ttaaccgcaa tcagactgac agtcattgaa
     5341 ggcctgattg caaaaacggg acaatcatga aaagaatcaa cagcagcgca ccggtaaaat
     5401 gcagcaagac ggtaatcatc agtgcaaaca gtgaaaaggt atgggcagtt ctgacagaca
     5461 taagcaactg gccagcctgg cagacagaca tcagcaaatc gttccttcac ggagatctga
     5521 aaccaggcag aaattttaac tggacatccg ggggagcaaa cattctctca acaatccata
     5581 cggtggaacc gttcagttac ttcggctgga caggcaaggc agttggactg ttcgctgtac
     5641 ataattggat attgatggag acaagcggac aaacaattgt cacggtggag gagagtatgg
     5701 aaggattcct tgcagtcctc ctgaggaagt cgctcaacag aaacctggag aagggtatgc
     5761 acaactggct cgccctttta aagcaggcgt gtgaaaagaa gcaagccata cctgcatgag
     5821 ccgaagcaac catagaaaaa ttattgagtc actaaaataa atgaataaca gtcatgaaaa
     5881 gatatacttt gaacagtgtt attttgttgc tgacactaac cctgctgatc agtaaaggtc
     5941 actctcaggt cattgtaaaa gacactctgc ctccctcttt tgaatggagc ctgcatctgt
     6001 ttgactttcc ctatatggtt gatgcggcaa aaaccgaagc catcagggca ggcggcggag
     6061 accttcccca gtcagtcaca ccgggtgccg gagattacgg caatttctac cgtaacctga
     6121 gcatggagca gacaactgag atggcccgga acctgcatgg ctccttatac tacgggcaca
     6181 atgtgatctg gaacgtagta attgaaccaa acagcacgaa gagatatatc ctgaaccgcg
     6241 tagtggcaaa tattgcagcc ctgggcacag attatcttgc cataaaactg ccttacggat
     6301 ttgcatacca gcatgaggag tttcatcgtg cggtaatgtc atcaagacat atctacagtt
     6361 atgacgaggt ctggaagttt ggcaaagggc tggatatcgc cgtaaccaat gtgaaggacg
     6421 aagacctggt ttacctcaag gcgaaccatc ctgctgacca ggtgagattg tcggcagccg
     6481 gcgttgaagg ggagtatgcc ttcctgctgc ggatgaggga agacaatttc ttcaggcaga
     6541 ccggctaccc gttcctcggt atttcactgc tgggtacaat ccatgccgtg agttatgtaa
     6601 acctgccttt taccgaaaga ttcaattcga taactgacag catccttgcg catgacaggg
     6661 acaatatact ggcaagggat ttcacgggct gggatttttc cgcctgggtt tatgatctgt
     6721 ttaccccgga ggagccgtat gaagaccgtg gtacatggcc cggaggtctg ggtataaaac
     6781 gccccgtgaa ggagtcagac ctgactgcgg agatgaaaga ttttctgagg gagacaggga
     6841 acatgcaata tctgaatttc atctcgccgt tcatgattgg tatcaacagg atccagctca
     6901 aaccaggcta cttctttaat ttcgccttgc gaagcatacc cacctccttt ggctattacg
     6961 ccggaggcga ttttttcctg gattttaacg accggcaggt gctgctgaac ctcgggatca
     7021 acagaagcaa aagtcttatc ctcccgtctg tggatgttac ttactataac attatcagga
     7081 gggaggattc acggttcagc gcgaatgtca atctctcagg atggcttcag ccaaaggatc
     7141 agctgtttgc cgccgacaag gccagcccag ggatggctgt cggagtgcaa ccccgttttg
     7201 atatcggaga gcgatttacc ataatggccg atttgtcata taaaaccaaa gggtgggtat
     7261 ttggcaaccc gtacctcgat gaaaagttga catttcgcct gggttttggt ttaaatacga
     7321 aaaattagtc ataacttttc ctgagaaatc agagcatctg caaaacaatg cagacagtgc
     7381 tatcgtgcct tatctgttgg gtgtccatta agaaggggga attatgatca aaaattcgtt
     7441 caggtggatt gcctctcatc aggtaacgtc ttttttcatc atcactttcc tgataacatg
     7501 gggacttggg ttttcataca tcgcggtaat gcgcgagaag tacctctttc tgccgttgat
     7561 gtttctggcg acgtgcggac ccgccattgc cggcatcatt gttgcatcca tttcggattc
     7621 ggaccggcag gcggcaagaa agcggttttc atggctcgcc ttgctgattt gctggatcgt
     7681 cgtgacaatg gcctttatta ccaatcagtt tttcgtcaat aaaggccagg tttcgacccc
     7741 acttgtgatc ctcatttcaa tctcctcact tccggttgca tttatcgtta atctcgccta
     7801 tacacggttc cccgttatac gtggtttcaa actgaagtca accgtttctg gcggtagtca
     7861 cacatggatc atcattgcat ttttactctt tccttgcctg acgataattt caatattggt
     7921 cagcaggata ttgggtcacg agacaccctc cctttccggc ctgccggcta ccggttttgg
     7981 cctggccgga ctgtttgcaa tcaaattcct gtcgcagttc ttttacttca acggaacagg
     8041 agaggaggtc ggttggcgag gctttgcgct tcgcaggatg caggcccggt ccagcccgct
     8101 ggttgccggc ctgatactgg ccctcttctg ggtgccgtgg cacatgtttc tgtggaccgg
     8161 ggaaggaagg gatgtcatga aagtatcttt ctgggcaagt tcgtacctgc ttcacatacc
     8221 ctcggcgata attctctgct ggtgctataa ccggagtcga ggcagcgtgc ttgcagcagg
     8281 tattgcccac gcatctgcta atacaattgt tgctttgatt cagccgcttg atcagaatat
     8341 catgattata gttttctttg tattcactgc agcaatagtt atccttgacc ggatgtggaa
     8401 gccgttgcct gaagaacatc cagccgtaca aaggatacgc gaatcctgag cttatcagga
     8461 tagacgcaag aatgtctgca tgaatctgcg aagggcttgt atgcatacga ggtcacaacc
     8521 aaaaattcaa tatatgaaag gggtattctt attcattttg ctggttgctg taatatcttg
     8581 taatacagat aaaaaattta cgattaccgg ttttacttcc agactgccgg acagcacggc
     8641 gatttacctg agaaaggcat ctgcttcaca aatctgtgat tcaacgatgc tctcaggcaa
     8701 cagtttccgg tttcagggag ctgttgattc aatcgaacaa tatgtagtgc ataccaggga
     8761 cttccaggat tatatatcat tatggattga caactctgag ataactattg atgcttcaaa
     8821 gtacaagctc cggactgcga aagtttccgg gtctgagttt caggagttga actcatctta
     8881 tctggaactg gagaacaatc tgaaggcgaa gaaagacagt attgatatga tcataagaat
     8941 cacaaacaaa tcagatactg tcagactgga atcgctttac agaatcaggg actcccttca
     9001 gacagtgaag cagaatgcaa tccttgattt tatggtttca aatcctgagt tctatctaaa
     9061 tccatattac ctcacattcc tgatgtacaa tcaaccattg caggtaactg aaaaaatgtt
     9121 taatgcgcta tctgtgcctt cacgggaagg ccattggggc aaagcgataa atacattcct
     9181 taatgagtct gttgagttga agacaggcga ccaggctatc gatttttcat tacccgatat
     9241 taatggaaac caggtgtctc tcaattcgta caggggcaaa tatgttttac tggaattctg
     9301 ggcttcatgg tgtggcccat gtcggctgga gaacccaaac ctgcttgacg cttacaggaa
     9361 atacagggat caggggtttg aaattcttgg cgtttcactt gatgagcaaa aaactgactg
     9421 ggagtctgcc atcagggaag ataccatcgg gtggacaaca gtgagtgacc tcagggggat
     9481 gctgggagac gttcctgtga ggtatagagt aaatggaatt ccgcagaact atctcattga
     9541 tcctcaaggg aaaattattt a
//
LOCUS       DAOPXU010000109         9493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_8493_length_9493_cov_11.129794, whole genome shotgun sequence.
ACCESSION   DAOPXU010000109 DAOPXU010000000
VERSION     DAOPXU010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8493_length_9493_cov_11.129794"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PKK03_10070"
     CDS             complement(<1..925)
                     /locus_tag="PKK03_10070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNX84787.1"
                     /translation="MKILKIAAVVGLLAVMPVIRAQQFPMYSQYMMNGFLLNPSYAGS
                     DYYTTFGLTVREQWLNLPHAPSTYAAAFQTRLLNDSYITKSTAVRKKISRPTKGGRVG
                     VGGYLFSDHNGIMHRTGIQLAYAYHLPVGDEQQLSLGLSLSAYQYFVDIRGAVMPDDV
                     QDELLNNYDQVVYIPDANFGVSYTTRRYYAGFAMTNLFRGTLMLGNGGENSRSELGHF
                     FLTGGMRFYPGADWIIEPSVMLKSSDMVFKSFQVDLTGRVYYKEDYWLGLSYRTGDAV
                     IMMAGVKVDRFYLGYAFDFTLSEIRSYTYGTH"
     gene            complement(946..6741)
                     /locus_tag="PKK03_10075"
     CDS             complement(946..6741)
                     /locus_tag="PKK03_10075"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HNX84788.1"
                     /translation="MKQIHLSTLVLALLLALPPQSADAQVGPGGVGTLTNTQLWLRSD
                     SLIAIQPLYYVTSWLDLSGHMRDFGAVIMGQTVPTLSLNTINGYPAVTFSDQGGVGGD
                     FLGYNGSIGISGSDGATVVIVARNTTAADEQNGGLYMGQKNAGGINAVRSYGLEHADA
                     VRFNGQNQVFNDGHTAGNWKIIYYSNPAGASVSAYGAFLNGTQLTGNSASAFVPSLVS
                     NFALVGATQMNGTYNPAGYFNGDMTEIAVFSGQLNDAERVVLQNNLGAKYLLPIADDH
                     YAWETTHSHDVSGIAAFNGTTFTNAWSTGMLSVTSPSGLSEGEYLFFGHNKAGAKTWT
                     TTEVPAPGTYRLPREWRFDETSDVGTVTISVPASSLPVLPSGYATVGILIDNDGDFTS
                     GATMYRPTLSGGNYTLDVNITDGQYLTIIAFRPEVNFTVPAGSGLENVTPASVQVSLN
                     YPHTSDVTVSYAATGGTATAGTDYTLVPGPVTIPAGSLTATFAINVINDAIVEPSETV
                     ITGLSNPSPGVSVGMQNSHTYTILNDDTVYAAFSSATASGPEGNGPAPVSSLRIVVSG
                     GITTVPGSVIVIVSNGTASSADWSQTGNMITIPAGDYTTPVSLPIPGAVLTIQGDLTV
                     EPDETVNVSMNTFVTVAAGPVVSSVYTILNDDNSTASVNAATTVIPEGGPGAAGTGTF
                     AFAFSNPVSNARTVSYTVTGTATSGTDFVALPGSFVMPAGALSYNLVLTSVADLLIEG
                     DETVRVTITGVSGAPAVTVNATPAVITIDDDDLPVILFSPVAVNMAEGSTATIEVWLQ
                     NAPAGPVTINVSTMMAGLLNISPASLTFNSSNFSTHQVITLQAIENAFLGDQTDNVIL
                     AVNDALSFDPFDPLPDIIIPVTIVNNDVASIVANPTSVMVAENGTATFTVTLSAAPPS
                     GDVVVDLVSNNTGVATINIAQLTFSAANYNVPRTITVSGVNNNTVPDASTTISLAVNN
                     ALSYDGYDGVTATVNVNVTNDDLAGFTVTPLTLTINEGGPAGQFTVVLNAQPLTDVVF
                     NLVNAAPVNVTHVSQVTFTPADWNVPVVVMVTATEDLLDADRTDVIAVTVNQALSDNS
                     FDALSSQNVTVNIEDNDPPVITGCPGNLSVNTSPGACSATVSWTPPVSTAPMTSTHLP
                     GSVFASGVTTVTYTSTDADGMVSTCSFTVTVTDAEMPVVNCKNITVNLDASGNATIAP
                     SDVLVSAPADNCAIAGVTLSRSAFTCADLGPVTVTVTATDASGNAASCDAVVTIADPF
                     VTSLNAGPDAEICTTQPSYTITGASVSNMTLSWSTSGSGTFSDPAALNPVYTRGATDL
                     TEVTLTLTGTKLNGCPETLTDAMTLTFAGVPAAGAGADKDLCSGTATVALSDATATNG
                     TISWTTSGNGTFSDPSIVTPVYTFGSSDTGPVTLTMTVTSASCGTVSDNLTITFTPEP
                     VAEAGPGGSVCRTEAGFQVAGASHAGGTVTWSTSGNGTFSDNTADNPYYTFGTADYAS
                     GSATLTMTVTGGGTCGTVTSSATVAINALPVPEVTSQQQITCNGLTDGAVQLAASGGL
                     APYTYSINGGPFQASGDFTGLAAGTYGFEVMDANGCIAGTTVDITEPMPFTAVIDGTD
                     DVTCHGSSDGAIYATLSGGTEPYSISWAGPDGYTASTATITGLEAGTYTLTVSDRYNC
                     ITLSFIEVITQPDAIKIISADISDNNGYGVTCPGSADGSITVVVQGGTPPLVLEWTGP
                     AGFTSDQPSISMLGAGEYMLTISDAAGCILTADYTLTAPEALDISAVTADASCPDTPD
                     GDIDLTVSGGSGTLAFLWNDGATVADRTDILSGDYSVDVTDVSGCTTTFEITVGVRGL
                     DCLRIYEIITPNGDGRNDTWQLRNAWLYPDAEVFVYTRWGKLVYHSGNATDEWDGTFN
                     GELLPNDSYHYVIHLNDGSEPRTGIISIISK"
     gene            7128..7202
                     /locus_tag="PKK03_10080"
     tRNA            7128..7202
                     /locus_tag="PKK03_10080"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7163..7165,aa:Val,seq:tac)
     assembly_gap    7204..7303
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8392..9348)
                     /locus_tag="PKK03_10085"
     CDS             complement(8392..9348)
                     /locus_tag="PKK03_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84789.1"
                     /translation="MKKMMYLFAALVMAAALGSCQQDDNEIVLKKGDSGSETVLPPSD
                     AGITPVVLEGANNGGNVTCAEVAAEFDLEEGYFLCGDKVDYTDGMFAGEFPDGLNVEV
                     KDGVYVSFSLDAPVIIEGVEYVVGAAIVKGGNSANVYFYPGGSMGDKNLTAPLNRSGK
                     PAGLSNLTFCLVKANPLVIALKTYLAVPVAGEQVSYKRIGWAVSGGLGVSTEYGLHMG
                     YNYYDFNGNNEFDLVKATLGAIEGPVGTIKASDYWEENEHFLEVVLEIDEAYNFVFDD
                     TYLYVGSLAGYEGRWYKAFPWSVEGIFAGSRVFRINFDEITM"
BASE COUNT         2354 a         2324 c         2382 g         2333 t
ORIGIN      
        1 catgtgtccc gtaagtatag ctgcggatct ctgaaagagt aaagtcaaat gcataaccca
       61 ggtagaacct gtcaaccttc acaccggcca tcatgatcac cgcgtcgccg gtcctgtatg
      121 agagcccaag ccagtaatcc tccttgtaat aaacccttcc cgtcaggtca acctggaacg
      181 atttgaacac catgtccgac gacttgagca taacagacgg ttcgatgatc cagtcggctc
      241 cggggtagaa tctcatacct ccggtcagga agaaatggcc aagctcggag cggctgtttt
      301 cacctccatt gcctaacatc agcgtacccc tgaaaaggtt ggtcattgca aaacctgcat
      361 agtatctcct tgtggtataa ctgaccccga aattggcatc ggggatataa accacctgat
      421 catagttgtt cagcagctca tcctgcacgt catcaggcat cacggccccc ctgatatcaa
      481 caaaatactg gtatgcgctg agtgacagcc cgagagaaag ctgttgctca tcccctacgg
      541 ggagatgata cgcatatgcc agctgaatgc ctgtcctgtg catgatgccg ttatgatctg
      601 agaagagata acctcctact cctacccttc cacccttggt gggcctgctg atcttcttcc
      661 tgactgcagt cgacttcgtg atgtaactgt cattcagcaa cctggtctga aaggctgcag
      721 cgtaggtgct tggcgcgtgg gggaggttaa gccactgctc gcggactgtc aggccgaatg
      781 tggtgtaata gtcacttccg gcatacgatg ggttaagcag gaagccgttc atcatgtact
      841 ggctgtacat cgggaactgc tgcgctctga tgacaggcat aaccgccaga aggccaacca
      901 ctgctgctat ttttaaaatt ttcatatctc cttccggtta tctggttact tgcttatgat
      961 cgagattatt cccgtccgcg gctcagagcc gtcgttcagg tgaatcacat agtggtaaga
     1021 gtcattgggg aggagctctc cgttgaaagt gccgtcccat tcgtcagtgg cattccctga
     1081 gtgatagacc agcttgcccc agcgggtata aacaaagacc tcggcatccg ggtagagcca
     1141 tgcattgcgc agctgccatg tatcattcct gccatcaccg tttggtgtta ttatctcata
     1201 tatcctcaga cagtcgagtc ccctgacccc aacggtgatc tcaaaggttg tggtgcatcc
     1261 gctgacatca gtcacatcga cggaatagtc tcccgacaga atatctgtcc tgtcagcaac
     1321 agtggcaccg tcattccaga ggaaggcgag tgttcccgaa ccgcctgata ccgtgaggtc
     1381 aatgtcgccg tcaggcgtat caggacagga ggcatcggcc gttacggccg aaatatccaa
     1441 tgcttcgggc gcggtaagtg tatagtctgc agtgaggatg caccctgccg catccgaaat
     1501 tgtcagcatg tactcgccag caccgagcat ggagatcgaa ggttggtcag atgtgaatcc
     1561 ggcaggacct gtccattcca gaacaagcgg aggcgttcct ccctgaacta ccacggttat
     1621 tgaaccgtct gcagacccgg ggcaggtaac tccgtaacca ttgttatctg aaatatcagc
     1681 agagatgatc ttaattgcat caggctgggt aatcacctct ataaagctga gtgttatgca
     1741 gttatatctg tctgagacag taagtgtgta ggtccctgcc tccaggccgg taatggttgc
     1801 ggtggaagcc gtatatccgt ccggtccggc ccagctgata ctgtaaggtt cagttcctcc
     1861 ggacagcgtt gcgtagattg caccgtcaga gctgccatga catgttacat catcagtacc
     1921 gtcaatcaca gccgtgaacg gcattggttc agtaatgtcc acagtggttc cggcaatgca
     1981 gccattggcg tccatcactt cgaacccata ggttcctgca gccaggcctg tgaaatcacc
     2041 ggaagcctgg aatggtcctc catttatgct gtatgtatac ggagcaagtc cgccggatgc
     2101 ggccagttgc actgcgccgt cagtcagacc gttacaggta atctgctgtt ggctggttac
     2161 ctcaggcaca ggaagagcgt tgattgcaac agtggctgaa gaagttaccg tgccgcaggt
     2221 tccgccacct gtaactgtca ttgtgagggt tgctgatcct gaggcatagt ccgccgtgcc
     2281 gaatgtatag taggggttgt ctgcagtatt gtcactgaag gtgccgttcc cgctggtgct
     2341 ccatgtcaca gtgcctccgg catgggaagc accggctacc tggaatccgg cctcggtgcg
     2401 gcatactgat ccacccggac cggcttctgc tacaggctca ggggtgaagg ttattgtaag
     2461 attgtcactc actgtcccgc atgaggcgct tgtgaccgtc atggtaagag tgacagggcc
     2521 ggtgtcagaa ctcccgaaag tgtaaaccgg agtgacaatg gacggatcac tgaaggtacc
     2581 attgcccgat gtggtccagg atatcgttcc gtttgttgcc gttgcatctg aaagggcaac
     2641 agtggcggtt ccgctgcaga ggtccttgtc ggctcccgca ccggctgccg gtaccccggc
     2701 aaaggtcagc gtcatggcgt cagtcagcgt ctcaggacac ccattcagct tcgtcccggt
     2761 cagtgtcaga gtcacctctg taagatcggt tgcacctctg gtataaaccg gattgagcgc
     2821 tgccgggtca ctgaatgtac cactgcccga tgtgctccat gacagggtca tattggagac
     2881 tgatgccccg gtgatggtat aggacggttg cgtagtgcat atttccgcat caggaccggc
     2941 attcagcgat gtgacaaacg gatctgcaat ggtaacgact gcatcgcaag atgccgcatt
     3001 gccgcttgcg tcagttgcag ttaccgttac ggtaacaggt cccaggtctg cacaggtgaa
     3061 ggctgaacgg ctgagcgtga caccggcaat cgcgcagttg tccgcgggag cggacaccaa
     3121 cacgtctgac ggggcaattg ttgcattacc ggaagcatcg agattaaccg ttatgttttt
     3181 gcaattcaca actggcattt cggcatcagt cacggttacc gtgaaactgc aggtcgaaac
     3241 cattccgtca gcatccgtcg acgtataggt cacagtagtc acacctgaag caaagaccga
     3301 acccggaagg tgggtggatg tcataggtgc tgttgacacg ggaggcgtcc agctgactgt
     3361 ggctgaacat gcgcccgggg atgtattgac cgataaattg cccggacaac cggtaataac
     3421 cggcggatca ttgtcttcga tattgacggt gacattctgt gatgacagtg catcgaagct
     3481 gttgtcggac aaagcctggt tgactgtgac tgcaatgaca tcagtacggt cagcatccag
     3541 gagatcctct gttgcagtga ccataaccac aaccggcaca ttccagtccg ccggtgtaaa
     3601 ggtcacctgc gaaacatggg tgacgttaac cggcgcagca ttgacgaggt taaaaaccac
     3661 gtcagtcagc ggctgggcat tcagcacaac agtgaactgg ccggcagggc ctccctcatt
     3721 gatggtcagc gtgagaggcg tgaccgtaaa ccctgccagg tcatcattcg tgacgttgac
     3781 attgacggtg gcggtcactc cgtcataccc gtcatatgac agtgcattgt ttacggcaag
     3841 ggagatagtg gttgatgcat ccggcaccgt gttgttgttt acgccggata cagtgatggt
     3901 ccgtggaaca ttataatttg ccgctgagaa ggtgagctgt gcaatattga ttgtagctac
     3961 cccggtgtta ttgctgacca gatcaaccac gacgtcgccg gatggaggtg cggctgaaag
     4021 agtaacggtg aaggttgccg ttccgttttc agcaaccatt acactcgtgg gattagccac
     4081 tatagacgct acatcattgt tgacaatggt gaccggaata ataatatcag gcaagggatc
     4141 aaagggatca aacgaaagag cgtcattcac tgccagtatt acattgtcag tctgatcgcc
     4201 caggaatgca ttctcaatcg cctgcagggt aatcacctgg tgagtggaaa aattcgacga
     4261 attgaatgta agtgaagccg gactgatatt cagcagccct gccatcatgg tggagacgtt
     4321 gattgtcacc gggccggcgg gcgcattttg tagccatacc tcgatggtag ccgttgaccc
     4381 ttcagccatg tttactgcta ccggggagaa cagaatgaca ggaaggtcat catcatcgat
     4441 tgtaataaca gccggtgtgg cgttaacagt aaccgccgga gctcctgaga ctcccgtgat
     4501 ggtgaccctt acagtttcat ctccctcgat aagcaggtca gcaacggaag tcagcacaag
     4561 attgtatgac agtgcgcctg caggcatcac aaaggatccc ggaagggcaa caaagtcggt
     4621 accggatgtt gctgttccgg tcacagtgta cgaaactgtc cttgcatttg aaacaggatt
     4681 gctgaaagca aatgcaaagg tgcctgttcc tgccgcaccg ggtccgcctt caggaatgac
     4741 tgttgttgcg gcattgacgc ttgctgtcga gttatcatcg ttgagtatgg tgtatacgga
     4801 actgacaaca ggacctgcag caacagtcac aaatgtattc atgctgacgt taacggtctc
     4861 atcaggttcc accgtgagat ctccctgaat ggtcaggaca gctcctggta tcggcaatga
     4921 aaccggtgtt gtataatcgc ctgcaggtat ggtaatcata ttgccggtct gagaccagtc
     4981 ggccgatgag gccgtaccgt ttgaaactat cacgataaca cttcccggaa cggtggtaat
     5041 tcctccgctg accactatcc tgagcgatga gaccggtgcg gggccattac cctcaggacc
     5101 tgaagcagtg gccgatgaaa atgcagcgta aacggtgtca tcattaagga tggtgtatgt
     5161 atgactgttc tgcattccta cagatacccc cggggaagga ttggagagac ctgtaatgac
     5221 tgtttctgaa ggctcaacaa tggcatcatt tatgacattg attgcaaaag tggcagtaag
     5281 agatcctgcc ggaattgtca cgggaccggg aacaagtgta tagtcagttc ctgctgttgc
     5341 agttccgccg gttgcggcat agctgacggt gacatcagac gtgtgaggat agttgaggga
     5401 aacctgtaca cttgcgggag taacattttc aagtcctgat cctgccggca cggtgaaatt
     5461 cacctccggg cggaatgcaa ttattgtcag gtactggccg tcagttatat tcacgtcaag
     5521 agtgtaatta cctcctgaca gggttgggcg gtacattgtg gctccggaag tgaagtcgcc
     5581 gtcattatcg ataagtatgc caactgtcgc atagcctgat ggcaataccg gcagtgagga
     5641 tgccgggacg ctgattgtca ccgtgccgac atctgaagtc tcgtcaaacc gccattccct
     5701 gggcagccgg taagtaccgg gagcaggcac ctctgtggtt gtccatgtct tagctccggc
     5761 tttgttatgt ccgaaaaaga ggtattcgcc ttcactgagg ccggaaggag aagtaactga
     5821 aagcatacct gttgaccagg cattggtgaa ggtagtgcca ttgaaagctg caatacctga
     5881 cacatcatga gaatgggttg tttcccaggc ataatggtca tctgctatcg gaagcaggta
     5941 ttttgcgccc aggttattct gaagcacaac cctttcagca tcgttcagct ggcctgagaa
     6001 gacggctatc tctgtcatgt caccgttgaa ataaccggcc gggttatagg tgccgttcat
     6061 ctgggttgct cccacaagag cgaagttgga aacaagtgaa ggaacaaagg ctgaggcaga
     6121 gttaccagtc agttgagtac cgttgagaaa cgcaccgtag gcagagacag acgcccccgc
     6181 agggttggaa tagtagatta ttttccagtt gcctgccgta tgcccgtcat tgaagacctg
     6241 gttctggccg ttgaatctga cagcatcagc atgttcaagt ccgtagcttc ttactgcatt
     6301 tatgccaccg gcattcttct ggcccatgta cagacctccg ttctgttcat cggcagcagt
     6361 ggtattccgg gcaacgatca caactgtggc tccgtctgat cctgaaatgc cgatggatcc
     6421 gttgtagccc agaaaatcgc ccccgacacc gccctgatca gagaatgtga cagccggata
     6481 accgttaatg gtattcagcg aaagtgtagg cacggtctgg cccatgatca ccgccccgaa
     6541 gtcgcgcata tggcctgaaa gatcaagcca gctggtgaca tagtacagag gctgtatcgc
     6601 aatcaggctg tctgacctga gccagagttg tgtattggtc agtgtgccta ctccgcccgg
     6661 gccaacctgc gcatcggcgg actgcggagg cagggcgaga agaagtgcaa gcaccagggt
     6721 tgacaggtgt atttgtttca tatcggtcag gatcagcgtt caggcaacat atcaaactac
     6781 ttatcaaaca gttgagtctg caatctttaa gcacttaccc ccggattgca actcatttat
     6841 ggtcaaaaag taccaaaagc taatataaat atattcctat aaacggaatc atgtgggtaa
     6901 agcgtataat taacagggtt aataaaatga ttcacagtgt ggccctatgg ttgtacgaag
     6961 acgattttga tttgttccgg aagggtgttt tatttttaaa accgctattg cggaataaaa
     7021 aattgaattt atatttgcac tctctttccg ggcgattagc tcagttggtt cagagcgtcc
     7081 cgacacgcaa gtgtcgggag ggtcgtaggt ccactgagat tgaaataggg cgattagctc
     7141 agttggttca gagcgcctgc cttacaagca ggaggtcgta ggttcgatcc ctacatcgcc
     7201 cacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttttttt ggttcagagc
     7321 gtcccgacac gcaagtgtcg ggagggtcgt aggttcgatc cctacatcgc ccacaatatg
     7381 aaattttttg ctaaatttag tgggatcttt tatgtacgct gtttacatta tatattcttc
     7441 taaactcgat aaatattata tcggctcctc ggctgacgta atcggcagac taagacgtca
     7501 caactctcat tcaaaaggat tcacttctgc aggaaggccc tggatattag tttacaccga
     7561 agaccattca aataaacatg atgcagaatc cagggaattg caactgaaaa agtggaagaa
     7621 tagagagagg ttgaaaaccc ttcttgataa aggtttagag ggtcatgaga cgcaaatccc
     7681 cgcagggtca taagtttgtc tcccgcatca acaatcttta agatttggca cttactctaa
     7741 tcatcacagt aaagatagtc ccaactggtt ttgaatttac tgatcctagc tcctacttaa
     7801 taatcttaaa attgtgtata ggtctgtaaa caaataaacc agccaaaatc attgtcgttg
     7861 cttcctgtaa aatcctacca taatgtcaga caaaaaaaag aggcagttcc tccccccacg
     7921 tacattttat aatagatagt aaagatgaaa aaattgtccg actagcgtac gtgctggaac
     7981 tgccgtgatc caaaaatata aaaaactaat tcaatagcct aatgaaaagc taaaatttcc
     8041 ggaaatatac actgaacgga actttttgcc tacagcgaaa tcatggaaac ttcgtctgtc
     8101 aatgcctgta atctgttgac catgtaatta cctatattag tcaaaaccag catattgccc
     8161 tattgccatg tcctgtatat tttgttactt tatacttccg aaggtccttt ttaattttga
     8221 aaggcagccc tcacaacttc agaaagttcc ccggagaggg gacagaaaag tgagggctgc
     8281 tgtcatttat gcaagtagcc ggatgtgacc gggtaaaaag cgtgaaggga tgcaaaaaaa
     8341 aagccggcca tcatggccga cccttttttt acaggatttt ttcggttcat cctacattgt
     8401 aatctcgtca aagttgattc tgaatactct tgaaccagca aagattccct caacagacca
     8461 ggggaatgcc ttgtaccacc tgccctcgta gccggcaagc gatcctacat agagataggt
     8521 atcatcgaac acgaagttgt acgcctcgtc aatttcgaga actacctcta gaaaatgctc
     8581 attttcttcc cagtaatcgg aagcctttat agtacccaca gggccttcaa tcgctccgag
     8641 tgtagccttt acaaggtcaa actcgttatt gccgttgaag tcgtagtaat tgtatcccat
     8701 gtgcagtccg tattccgtgc tgacacccag tccgccactt acagcccatc cgattctttt
     8761 ataggagacc tgttctcctg caaccggtac tgccaggtag gttttgagcg caatgacaag
     8821 cggattggct ttgaccaggc agaatgtcag gttgctcagg ccggctggct tgccggatct
     8881 gttaagaggt gcggtaagat tcttgtcacc catgctgcct ccgggataaa aatatacatt
     8941 ggcagagttg cctcccttga caattgctgc gccaacaacg tattccacac cttcaataat
     9001 gacaggcgca tcgagactga agctcacata aacgccatcc ttcacctcca cgttcagacc
     9061 atccggaaac tcaccggcga acattccgtc ggtatagtca accttgtcac cgcagaggaa
     9121 atatccttcc tccaggtcaa attcagcagc cacttcagcg catgtaacat tacctccatt
     9181 attggcacct tccaggacca caggcgttat acctgcatct gaaggcggaa gtactgtttc
     9241 agaaccagaa tctccctttt tcaggacaat ttcgttgtca tcctgctggc aggaacccag
     9301 tgcagctgcc attacaagag ccgcaaacaa atacatcatt tttttcattt cagccatttt
     9361 tagtgaataa ctaataatca tttattgttt gtaatatttt taaatatgtc tgtcacgcaa
     9421 tcagtcctat cctggcagac ctgcgccttg catggaaata ggtcaggccg tttttctcgg
     9481 tcatggtaat cag
//
LOCUS       DAOPXU010000110         9465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_8533_length_9465_cov_11.831031, whole genome shotgun sequence.
ACCESSION   DAOPXU010000110 DAOPXU010000000
VERSION     DAOPXU010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9465
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8533_length_9465_cov_11.831031"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..555
                     /gene="nuoE"
                     /locus_tag="PKK03_10090"
     CDS             88..555
                     /gene="nuoE"
                     /locus_tag="PKK03_10090"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNX84790.1"
                     /translation="MSKIDDILSRYREGSHESLIPILQDVQEEEGYLTESAIVRIGSF
                     LRMSTTKIYGLATFYDRFRFIPAGRVHMRICNGTTCFINGSAAVVTAVRETLGIEPGQ
                     TTRDGRFSYELTACQGGCNEGPVICIGENYHTRVRPEDVPELIAKLKSLSDLR"
     gene            552..2501
                     /locus_tag="PKK03_10095"
     CDS             552..2501
                     /locus_tag="PKK03_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNX84791.1"
                     /translation="MKLTKDSGIKDFLINEVLMHASDTLPAETSARLAGIRRDKPSVP
                     VIYVATGSAAIIAGSNATAKATRQYLDEMGMKGEVVRTGCHGPAAFEPLFCVHLPGKN
                     KLIFRNVTEDKVEPLLNGVFHNDMPVEDLVAQSGTYGFEAWHEIPFLDEVPFFRMQKR
                     VVLGNCGCYDPESIEEYIARGGYRAFLKTIRNYTHEEVCDIVERSGLRGRSGGGFNTG
                     LKWKYALNSASDNRYLICNAKESDPGSYADRSVMEGDPHRLIEGICIASYAIGASTAI
                     IYLKTGSPHPLARLRKALEAASEFGITGHNILSSGFNLEILIREEAGAFVCGEETALI
                     ASLDGKRGMPELKPPYPTTSGLYGKPTVINNVETLMNVSLIMSHGPEWFRSMGTDGSK
                     GTKLFSLGGRGRCTGLIEVEMGTTFRTILEGIADGIREGKAFKTLQIGGPSGTFITEK
                     NLDLPVDFDVLRANGIAMGSGGLIIIDETTCLVDFVRYYMEFIHRESCGKCIPCREGT
                     GRMLEILENVVRKPLNEDSNATLERFKGVMQLETIASVMKDTSLCGLGQTAPNPFISA
                     LDNYREEFEEHIFDRRCRANVCRGLRTFSIDVEKCTGCTVCAAKCPVNAIYGTRLQPF
                     FIVEDKCIGCGVCYDVCKFSAVTVK"
     gene            2505..4220
                     /locus_tag="PKK03_10100"
     CDS             2505..4220
                     /locus_tag="PKK03_10100"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNX84792.1"
                     /translation="MQFTIEVNNKSIRAEKGETILSALNRNGIIIPTLCRMAEFTPTG
                     ACRMCVVEVEGRDRLVTACSAPVEEWMKIKTHSPRVIRARKTIVELLLSNHPDDCLYC
                     DRNLDCELQRLSDEMQVRERRIRGSKIKPRRDQSSPAIVRELSKCILCGRCLRVCEEV
                     VTATSIDFIGRGRYTHVGPAMDRDFNFSSCIHCGQCVLVCPTGALHEKHYLSEVQEQL
                     SKGDVTRVIQYSPLVPYALAEELGVKYNRDFDRLLNAALRKTGFDRIFLSGAGTDILI
                     TELTDAILSSRNKPDRKPLIVSACPAWVKYCEQFLPELLPMLSTLKTPQQISGAIIKS
                     VVPASNDKSRIYTVSVTPCTAMKFEARRDSMTRKGISDIDTVLTVRELARLIVLYGID
                     ITNIDPEPADEPMALRSSSSLLAESAGGITESVLRSLAARMGEKDAEKPALKKLRNTV
                     SFRETMVTAGGESFSVAVVDGLKGLTRIRQMIEGGAKYDLIEVMACPGGCVNGGGMLP
                     AGSKEAVKQRSRFIWQTDDQEAVRGPEQSAAVADLYGRVFEQCTELADKKLFHTHFSK
                     RDILL"
     gene            4234..6204
                     /locus_tag="PKK03_10105"
     CDS             4234..6204
                     /locus_tag="PKK03_10105"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNX84793.1"
                     /translation="MLVYTIKELCRTCYTCVRECPARAIRIVGGQAEVITEKCIACGN
                     CTKVCSQGAKVFVNTTDLVRKVLARPGKRAAIVAPSFPAEFHDVPDYLRVVGMIRALG
                     FDLVSEVSFGADLVAHRYRNLVSQAGEKNYISSDCPAIVSFIRFYHSDLTAELIPVVS
                     PMVAMTRVMRKKYGDDLSVVFIGPCVAKKAENAEVDAAITFIELREMLQESGIKPETA
                     EPSEFDPPLGGRGAIFPVTRGLLQTAGVNDDAITGNIIAAEGRIGFQEAIKEYEQGMI
                     GGQHLELLCCEGCIMGPGMSKGGKQYNRRAQVSAYAQKKMAGLDMEEWQRNLDLYDRL
                     DLSVRYRPEDKRQLIPPDDNEEVRQVLASMGKLTEKDHLNCGACGYDTCYDHAIAIIK
                     GLAEEEMCLPYTIEKLHKSVQDLALSNAKLTTMQNALKQSEKLAHMGQLSAGIAHELN
                     NPLGVVIMYSNILLDESKPDDPVREDLQLIVEQAARCKKIVGGLLNFARKNQVNHQFI
                     DIRKLTENSVAGVVFPDNVKAVIEDRTTSPDASIDSEQMTQVLTNLFRNAIDAMPGGG
                     TLTVTLEDTPSDVIFIVSDTGSGIRDEDKPKIFEPFFTTKGLGKGTGLGLATTYGIVK
                     MHKGQITVETSNDPSKGPTGTTFRIILPRRPE"
     gene            6246..6638
                     /locus_tag="PKK03_10110"
     CDS             6246..6638
                     /locus_tag="PKK03_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX84794.1"
                     /translation="MSKIYTVLLADDDPDYLFQVAFYLRKAGFEVMAVESQAEAEQVI
                     AKMKPDIAVFDLMMESDDSGFILCYKIKRKYPEVPVVLATAVSRETGITFGLSGGQDR
                     EWIRADLYLEKGVRPEQLEQELRKLLKI"
     gene            6641..7789
                     /locus_tag="PKK03_10115"
     CDS             6641..7789
                     /locus_tag="PKK03_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNX84795.1"
                     /translation="MAQLKILVVDDEPGIRSGVSRILGNFHVTYPFMDEDYTFNILEA
                     ATGEEGIEIIDREMPDILLLDNKLPGIQGVEVLEYVRKKNYDIVVAMITSYASIDVAV
                     KAHNDGAIDFIPKPFTPQELKASIEQITKQQYLRRITHKLKVEGRKVRFQFLSVLSHE
                     LKAPLNAIEGYLRMMQERQMGDSLDEYASAIERSLQRIESMRNLIMDLLDFTKVSFER
                     QMEKMQNVNLKEMVSMAVVTVSPYAIHKDIRFETSINGCETIWADPNDFEIILNNLVS
                     NAVKYNKPGGTVTVTVDCNENEFTIRVADTGIGMNNEERDMLFEEFSRIRNDKTRNIS
                     GSGLGLSIVKKVVDLYHGVIDVESSPDRGSAFTVIIPRSQVSNLKTGI"
     gene            7786..8421
                     /locus_tag="PKK03_10120"
     CDS             7786..8421
                     /locus_tag="PKK03_10120"
                     /inference="COORDINATES: protein motif:HMM:NF003996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNX84796.1"
                     /translation="MKTLPGKTVERLSQYRRALASTLAANRNYVFSHELAAMLHITAV
                     QVRRDLMLIGYGSVMRKGYDVRELINTIGSIIDAPSGLNVAIIGMGNLGRAITGYFRG
                     KRTNMNVVAAFDVDPQKIDKVVAGVRCYNISQIRKLREELDIMVAVLAVPADQAVATA
                     SQLVSNGIKGIMNFTTVPLNVPDAVYLEEFDMITSIEKVAYFVKENQQPAG"
     gene            8421..9332
                     /locus_tag="PKK03_10125"
     CDS             8421..9332
                     /locus_tag="PKK03_10125"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HNX84797.1"
                     /translation="MRIFRSFQEASVINAPVVTTGTFDGVHIGHRTIIKRLKKLASDY
                     GGESVLITFHPHPRVVLYPETAGKGLKLICSQEEKIELLRKAGLDNVIIIEFTRDFSK
                     VTSEEFVRDILCGVLGAKVIVVGHNHHFGFNQEGDYRTLWEWREKYGFEAEEIPMQEV
                     QNETVSSTRIRQALKEGYIQRANAYLDHFYLVIGTAVKDDPGLAPLVPMVRIPVTDVT
                     KLVPAPGLYAVSCAAETYFSRGMVCIPESTGLLPDLFLHLENYESDIPGKRMTLFFHK
                     RLDASRLASDPAKALVAGAEEMKELIF"
BASE COUNT         2437 a         2402 c         2601 g         2025 t
ORIGIN      
        1 tatttgttaa taaaataaca atgtttttga aataacaata aaaataaata gctttgtatt
       61 tgtctgaata gtcagatcgt ttttaaaatg agcaagatag atgatattct gagccgttac
      121 agggagggga gtcatgagag cctgataccc atccttcagg acgtccagga ggaagagggt
      181 tacctcaccg agagcgctat tgtgcgcatc ggcagtttcc tccggatgtc cacaaccaag
      241 atatacggcc tggccacctt ctatgaccgt ttcaggttca tcccggctgg cagggtacac
      301 atgcgcatct gtaacggaac gacctgtttc ataaatggtt cggctgccgt ggtgactgcc
      361 gtgagggaga cgttgggcat agaaccgggg cagacgacac gtgacggcag gttcagctat
      421 gaactgactg cctgccaggg cggatgcaat gaagggccgg tgatctgcat aggtgaaaac
      481 taccatacca gagtacggcc cgaggatgtc cctgaactta ttgcaaaact aaaatcactt
      541 tctgacctgc gatgaagctg acaaaagatt ccggcataaa ggatttcctc ataaatgagg
      601 tgctgatgca tgcctctgac acacttccgg cagagacctc cgcgagactg gccgggataa
      661 ggcgtgacaa gccatcggtc ccggtaatct acgtggccac cggctcggcg gcaatcattg
      721 ccggttccaa tgccactgca aaggcaaccc ggcaatatct ggatgagatg ggcatgaagg
      781 gtgaggtggt gcgtaccggc tgccacggcc ctgcagcctt cgagcctctt ttctgcgttc
      841 accttccggg taaaaacaaa ctcatcttca ggaatgtcac tgaagacaag gtggagcctt
      901 tgcttaacgg cgtatttcat aatgacatgc ccgtggagga tcttgtcgca cagtcaggca
      961 cctacggttt tgaagcctgg catgagatcc ccttcctgga cgaggtgccg ttcttcagga
     1021 tgcagaagag ggttgtgctt ggcaattgcg gctgttatga cccggaaagc attgaagagt
     1081 acattgcacg cgggggctac agggcgttcc tcaagacaat acgcaattac actcatgaag
     1141 aggtctgtga catcgttgag cgcagcgggc tccgcgggcg gagcggtggc ggcttcaaca
     1201 ccgggctgaa atggaagtat gccctcaact cagcttcgga caacaggtat cttatctgca
     1261 acgctaagga gagcgatccc ggttcatatg ctgaccggtc agtaatggaa ggtgaccctc
     1321 accggcttat agagggtata tgcattgcct cctatgccat aggcgcttca acagcaataa
     1381 tttatctcaa gaccggttca ccacacccgc tggcacggct tcgcaaggcc ctcgaagctg
     1441 cttcagagtt cggcatcaca ggccataata tcctttccag cggattcaac ctcgagatac
     1501 tcatcaggga agaagcaggg gcattcgttt gcggtgaaga gacagcgctc atcgcaagcc
     1561 tcgacggaaa acggggaatg cccgaactca agccgcctta tcccaccaca agcggactat
     1621 acggcaagcc gacagtcatc aacaacgttg agacactgat gaatgtgtcg cttatcatgt
     1681 ctcacggtcc tgagtggttt cgctctatgg gcactgatgg aagcaagggc accaaactct
     1741 tctccctcgg aggaagaggc cgctgcacag gcctgataga agtggagatg ggtactactt
     1801 tccgaaccat cctcgaggga atcgccgacg gcatcaggga gggaaaggca ttcaaaaccc
     1861 tccagatagg gggtccctcc ggcaccttca ttacagaaaa gaatcttgac ctgccggtcg
     1921 acttcgacgt gttgcgggca aacggcattg ccatgggatc aggcgggctg attatcattg
     1981 acgaaacaac ctgccttgtc gatttcgtca ggtactacat ggagttcatc cacagggaga
     2041 gttgtggcaa gtgtatcccc tgccgcgagg gcaccggaag aatgcttgag atactggaaa
     2101 atgttgtccg caaaccgctg aacgaggact ccaacgcaac gctggagcgg ttcaaggggg
     2161 tgatgcagct ggagaccatc gcttcggtga tgaaggacac ttcgctctgc ggactgggtc
     2221 agacagctcc caatcctttc atcagtgccc ttgacaacta ccgcgaggag ttcgaggagc
     2281 atatctttga ccgccgctgc cgggccaatg tctgccgggg cctgagaacc ttcagcattg
     2341 atgttgaaaa atgtaccggg tgcactgtct gtgctgccaa gtgccctgtc aatgccattt
     2401 acggcaccag gctgcagcct ttcttcatcg ttgaggacaa gtgcatagga tgcggggtat
     2461 gttatgatgt ttgcaaattc agtgccgtca cagtaaaata agccatgcag ttcactatag
     2521 aagtaaacaa caaaagcatc agggcggaga agggtgaaac catcctttcg gcgctcaacc
     2581 gcaacggcat tatcattccc actctatgcc gcatggcgga gttcaccccc acgggcgcct
     2641 gccgcatgtg cgtggtcgag gttgagggcc gtgaccgcct ggtaactgcc tgttcggcac
     2701 cggttgagga gtggatgaag atcaaaaccc attcgccccg cgttataagg gccaggaaga
     2761 caatagtcga gctgctgctc tccaatcatc ctgatgactg tctctattgt gaccggaacc
     2821 tcgactgcga actgcagcgc ctctctgatg agatgcaggt gagggaacgg cgcatacgcg
     2881 gaagcaagat caagccccgc cgcgaccagt cgagccccgc catagtacgc gaactctcaa
     2941 agtgcatcct ctgcggccgc tgtctcaggg tctgcgagga ggtggtcacc gccacctcaa
     3001 tcgacttcat cggccgcgga agatataccc atgtgggccc ggcaatggac cgcgacttca
     3061 acttctcgag ctgtatccac tgcggacagt gcgtgctggt atgccccacg ggagcacttc
     3121 atgagaaaca ttacctctcc gaagtgcagg aacagctcag caaaggcgat gtgacaaggg
     3181 ttattcagta ttcgccgctt gtgccctatg ctcttgcgga agagctgggg gtgaagtaca
     3241 accgcgactt cgacaggctg ctcaatgctg cgctcagaaa aaccggtttt gacaggatat
     3301 tcctttcggg agcggggacg gatatcctta ttactgaact taccgatgct atcctcagct
     3361 ccaggaacaa gccggacagg aaaccactta ttgtttcagc atgtccggcg tgggtcaagt
     3421 actgcgaaca attcctccct gaactgcttc ccatgctctc aacactgaaa acaccccagc
     3481 agatctcggg cgctataatc aagtcggtcg tccccgcaag caatgataag tcaaggattt
     3541 ataccgtctc tgttacaccc tgtactgcaa tgaagtttga ggcacgccgt gacagcatga
     3601 ccaggaaggg aataagcgac attgacacgg tgctgacggt gagggaactg gccaggctga
     3661 tagtcctgta cggcattgat attacaaaca ttgatcccga gcccgctgat gagcccatgg
     3721 ccctgcgcag ttcctcctca cttcttgccg agtcggcagg aggcattaca gaatctgtcc
     3781 tccgttcact ggcagcacgc atgggagaga aggatgcaga aaaacctgcc ctgaaaaaat
     3841 tgcgcaacac cgttagcttc cgcgaaacaa tggtcacagc cggaggtgag agtttttccg
     3901 ttgcagtggt tgacggactg aaaggcctca ccaggatcag acagatgatt gagggtggtg
     3961 caaagtatga cctcattgag gtgatggcat gccccggcgg atgtgtcaac ggcggcggaa
     4021 tgctgcctgc cggatcaaaa gaggctgtga agcagagaag caggttcata tggcagactg
     4081 acgaccagga ggctgtccgc ggtcccgaac agtcagccgc tgtggccgat ctctatggca
     4141 gggtatttga acagtgtacg gagcttgctg acaagaaact gtttcatacc catttcagca
     4201 agcgggacat attgctttaa aagaagaaag gctatgctgg tatatacgat aaaagagctg
     4261 tgccgcacat gctacacatg cgtgagggag tgccctgccc gtgcaatccg gatcgtcggg
     4321 ggacaggctg aggtgataac cgagaagtgc atcgcctgcg gcaactgcac caaggtttgc
     4381 agccagggcg ccaaggtctt cgtcaacacc accgaccttg tcagaaaggt actcgcccgg
     4441 ccagggaaga gggctgcaat agtagcccca agttttcccg cagagttcca tgacgtgcct
     4501 gattatctca gggtcgtcgg gatgatcagg gcacttggct ttgacctggt ctccgaggtc
     4561 tccttcgggg cagacctggt ggcgcatcgc tatcgcaatc tcgtcagcca ggccggtgag
     4621 aaaaactata tctcatctga ctgcccggca atagtctcat tcataagatt ttaccattca
     4681 gacctgaccg ctgaactgat ccctgtggtc tctcccatgg tggcaatgac ccgggtcatg
     4741 agaaagaaat acggcgatga cctgtcagtt gttttcatag gtccctgcgt tgccaagaag
     4801 gctgaaaatg cagaggtcga tgctgccata acgttcattg aactgcgcga gatgctgcag
     4861 gaatctggaa taaaaccgga gacggctgaa ccttcggagt tcgatccgcc gctgggcgga
     4921 aggggcgcca tcttcccggt gacaaggggt ttgctccaga ctgccggcgt caatgacgac
     4981 gccattaccg gtaacattat tgcagctgaa ggaaggatag gattccagga ggcaatcaag
     5041 gagtatgagc aggggatgat tggcggacag cacctggaac tgctatgctg tgagggatgc
     5101 atcatgggcc ccgggatgag caagggtggc aagcagtata accgaagggc gcaggtgagc
     5161 gcctatgccc agaaaaaaat ggccggactg gatatggagg agtggcagcg aaacctagat
     5221 ctgtatgacc ggctcgatct gtcagtccgg taccgcccgg aagacaagcg gcagcttatc
     5281 cccccggatg acaatgaaga ggtgcgccag gtcctcgctt ccatgggcaa actcacggaa
     5341 aaggaccacc ttaactgtgg tgcatgcggt tatgacacct gctatgacca cgcaattgcc
     5401 atcataaagg gactcgccga ggaggagatg tgccttccat ataccattga gaaactgcac
     5461 aagtcagtcc aggacctggc tctttccaat gccaagctga ccaccatgca gaacgccctc
     5521 aaacagtcgg aaaagcttgc ccatatggga cagctctcgg ccggcattgc acatgaactc
     5581 aacaaccccc tgggagtggt gatcatgtac agcaatatcc ttcttgatga gagcaaacct
     5641 gatgatcctg tgcgcgagga cctgcaactt attgtggaac aggcagcaag atgtaagaag
     5701 attgtcggcg gactgcttaa cttcgcccgc aagaaccagg tgaaccacca gttcatcgat
     5761 atcaggaaac tcaccgaaaa cagcgtggcc ggcgtggttt tccctgataa cgtaaaagcc
     5821 gtcatcgaag accgcacaac cagtcccgat gcatctattg actccgaaca gatgacccag
     5881 gtgctcacca acctcttcag gaatgcaata gacgccatgc ctggtggcgg cacactgacg
     5941 gtaacccttg aggacacccc cagtgatgtc atcttcatcg tctccgatac cggcagcggg
     6001 atcagggatg aggataagcc gaagattttc gaacccttct tcaccacgaa gggcctgggc
     6061 aagggaaccg gcctgggact tgcaacaact tacggcatcg ttaagatgca caagggccag
     6121 attacggtcg aaaccagcaa tgacccgtca aaaggcccca ccggcaccac attcagaatc
     6181 atccttccga ggagacccga atagtgccgc ttaacagaaa tgaataaaca aaacaaacat
     6241 caataatgag taaaatttat acagtcctgc ttgctgacga tgaccccgac tacctcttcc
     6301 aggtggcttt ttacctgcgc aaggcgggct tcgaggtgat ggcagtggag agccaggctg
     6361 aagctgagca ggtaattgca aagatgaaac ctgacatcgc cgtctttgac ctgatgatgg
     6421 aaagtgatga cagcggcttt atcctctgct acaagatcaa gaggaaatac cccgaggtgc
     6481 cggttgtcct tgccactgct gtctcaaggg agacaggcat aaccttcggg ctcagcgggg
     6541 gtcaggaccg cgagtggata agggctgacc tttaccttga gaaaggggtc aggcctgaac
     6601 agcttgaaca ggagttaaga aagcttctta agatctgacc atggcacagc tgaagattct
     6661 tgttgttgat gacgaaccgg gcatcagatc aggcgtttca aggatcctgg gtaactttca
     6721 tgttacatat ccatttatgg atgaggacta taccttcaac atactggagg cagccacagg
     6781 tgaggaaggg attgagataa ttgaccgtga gatgcctgac atactcctgc tcgacaataa
     6841 gctgccgggt attcagggcg tggaagtcct tgagtatgtg aggaagaaga attatgacat
     6901 cgttgtggcg atgataacct cctatgcctc aattgacgtc gccgtaaagg cgcacaatga
     6961 cggggccatt gacttcatac ccaaaccatt cactccccag gagcttaaag cttcaataga
     7021 gcagatcacc aaacagcagt acctgcgcag gataactcac aagctcaagg tggaggggag
     7081 aaaggtgcgg ttccagtttc tgtcagtgct gtcgcacgaa ctgaaggcac cactcaatgc
     7141 cattgaaggt tacctcagga tgatgcagga gaggcagatg ggtgattcac tcgatgaata
     7201 tgcatcggct attgagagat cactccagcg aatagagagc atgaggaatc tgataatgga
     7261 ccttctcgat tttacgaagg tgagtttcga acggcagatg gaaaagatgc agaacgtgaa
     7321 cctgaaggag atggtgtcaa tggccgtggt taccgtcagc ccgtatgcca ttcataagga
     7381 tatccgcttt gaaacatcaa tcaacggatg tgagaccatc tgggccgatc cgaacgattt
     7441 cgagataatc cttaataacc tggtatccaa cgccgtcaag tacaacaagc ccgggggaac
     7501 ggtcacagtc accgttgact gcaatgagaa tgagttcacc atcagggtgg cagataccgg
     7561 catagggatg aacaatgagg agcgggacat gctgttcgag gagttctcga gaatccgcaa
     7621 tgacaagacc cgcaatatca gcggaagcgg actgggacta tcgatagtga agaaggtggt
     7681 tgatctctat catggggtga tcgatgtaga gagttccccc gacaggggat cagccttcac
     7741 ggtgataata cccaggtcac aggtttccaa tttaaagaca ggtatatgaa aacactcccc
     7801 ggaaagaccg ttgaacgtct cagccagtac aggagagcgc ttgcctccac cctggctgca
     7861 aacagaaatt atgttttttc acatgagctc gccgcgatgc tccacattac cgctgtgcag
     7921 gtcaggcgcg acctcatgct tatcggatac ggcagcgtga tgcgtaaagg atatgatgtg
     7981 agggaactta tcaatacaat cggatcaata atcgatgccc ccagcggact caacgtggct
     8041 attatcggga tgggcaacct gggaagggcc atcaccggct acttcagggg caagcggaca
     8101 aacatgaacg tggtggccgc ctttgacgtt gacccccaga agattgacaa ggtggtggcc
     8161 ggggtgcggt gctacaacat aagccagatc aggaagctga gggaggagct tgatatcatg
     8221 gttgccgtgc tggccgttcc ggctgaccag gccgtagcca cagcctccca actggtcagt
     8281 aacggaatca agggcatcat gaacttcaca acagtgcctc tcaatgtacc tgacgccgtc
     8341 tatctcgagg agtttgacat gataacctcg atcgagaagg tggcctactt tgtcaaggaa
     8401 aaccagcagc ctgcagggta atgagaattt tcaggagttt tcaggaggcc tcggtcatca
     8461 atgcacctgt cgttaccaca ggcaccttcg acggcgtcca catcgggcac cgcacaatta
     8521 ttaaacgact taaaaaactt gcctctgatt acggcggcga gagcgtgctc ataaccttcc
     8581 atccgcatcc cagggtagtg ctctaccccg agactgccgg caagggactt aagctcatct
     8641 gctcccagga ggaaaagatc gagcttctcc gaaaggcagg gctcgacaat gtcattatca
     8701 ttgaatttac gcgtgatttt tcgaaggtca ccagcgagga gttcgtgcgt gatatactct
     8761 gcggtgttct tggcgccaaa gtgatagtcg tagggcataa tcaccacttc gggttcaacc
     8821 aggagggcga ttaccggaca ctctgggagt ggagggagaa atacggcttc gaggctgagg
     8881 agataccgat gcaggaggtg cagaatgaaa ccgtcagctc aacgcgtatc aggcaggcac
     8941 tgaaggaagg atacattcag cgtgccaatg cataccttga ccacttctac cttgttatag
     9001 gcacagccgt gaaggatgac cctggcctgg cgccgctggt tcccatggta cgaattccgg
     9061 taactgatgt gaccaagctt gttccggcac ccggactcta tgctgtctca tgtgccgccg
     9121 agacctactt ctcaaggggg atggtctgta tacctgaaag caccggtctg ctaccggacc
     9181 tctttctgca ccttgagaac tatgagagtg atatcccggg aaaaagaatg actctcttct
     9241 ttcataaacg gctggatgcg agccgcctgg cttcagatcc ggcaaaagca cttgttgcag
     9301 gcgctgaaga gatgaaggaa ctcatcttct gaccggactg aacaatcaga ctcctttttt
     9361 cgtatctttg tatgttaatt tctgagcggg ggcataatta aaattgtaga gaaatgaaat
     9421 tcaatcctga aaaatgcagt atacctgacc gcaggatgga gccgt
//
LOCUS       DAOPXU010000111         9390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_8623_length_9390_cov_8.789716, whole genome shotgun sequence.
ACCESSION   DAOPXU010000111 DAOPXU010000000
VERSION     DAOPXU010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9390
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8623_length_9390_cov_8.789716"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="PKK03_10130"
     CDS             complement(<1..1088)
                     /locus_tag="PKK03_10130"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNX84798.1"
                     /translation="MAGLPAESGSTGGKKKITKVWLIGDSTVADYSLEKDYRTKRYPI
                     MGWGQVFQSFMAPDSLKLIRNLIGSDSAVVDDRARGGRSTRSFFEEGRWSEIYRQLRP
                     GDLVLIQFGHNDASVNKGERYTSLTGYKEFLRLYVSQSREKGAIPVLVTPVARNYPWK
                     DGVLGNTHGAYPDAMKEVAAETGAGLIDLNSLSMDYFSRRGRDYVTMTYYMNFPPGLY
                     EAYPDGSSDNTHFQPAGATVVAQLVFNAMKDLKPGKAGKPSALTVQVAPQVLSSPAGE
                     IYRDIEFTMPQVIEPQIAGNTVSIAEFGAVGDGVVLNTEAFRKAIESVSSRGGGTVVI
                     PRGIWLTGPIILKSNLRMHAEEGALVLF"
     gene            complement(1149..2759)
                     /locus_tag="PKK03_10135"
     CDS             complement(1149..2759)
                     /locus_tag="PKK03_10135"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HNX84799.1"
                     /translation="MKTADAIIINLVFQAAIMTGCNRSQSTDTGAVYTNPVLHSDYSD
                     PDVIRAGEDYYMVASSFNCVPGLPVLHSKDLVNWELTGYALDRLLPEEYYSTVRHGGG
                     VWAPCIRERNGEFYIFYPDPDHGIYMVKASGPGGPWSLPVMVMQGNGLIDPTPLWDDD
                     GSAYLAYAFAGTRAGVKSVLMVIRMKPDGTGVTGDPVMIFDGHDDNPTVEGPKFYKRN
                     GYYYIFAPAGGVTNGWQLALRAQKVFGPWEHRVVMHRGKTPINGPHQGAWVTTPGGED
                     WFIHFQEKGAYGRIVHLQPMKWVDDWPVIGIDDDGDGAGEPVTYFAAPRVGSTASPAA
                     IDTGDEFDSSVRPLNWQWPANPSLTWGYPSEAGGFYRLNCIARPDSMRNMWEVPNLFL
                     RKLPAEKFTATASVKCNLRSVGEEAGMIVVGRDYRYISVRRTDDRLLLRVVNCTDADA
                     GSVETELLSREVPTGDLFFRLTVESGASCRFSYSIDGNEFTDAGESFPARQGMWIGAK
                     VGFFALRDGFTNDAGSIDLNWFRILTTN"
     gene            complement(2764..4788)
                     /locus_tag="PKK03_10140"
     CDS             complement(2764..4788)
                     /locus_tag="PKK03_10140"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNX84800.1"
                     /translation="MMRRQIHIPVCKALLLTAFFFVVAAGLKAQDQNKVADWKTEFTL
                     RSHLLQEMHSLYDLRRERLREALSDECAINEYKAGCRGSYLRLLGELPPETPLNPEIT
                     GRIALGSIVLEKVIIESRPGHHVTANLYLPAGEGPFPAVLFFCGHEMTSKATLSYLET
                     ALLFAANGISVMVVDPISQGERVQFIDSEGRRILRGSTTEHTLLNAGAILTGSSVAAW
                     ELHDNIRALDYLVSRPEIDSNRIGCMGNSGGGAQTMWFIGFDDRVKAAAPCSFITTRE
                     REYELNGTGDGCQQIAGEAEAGLEIADYPAIFAPKPLLILAGRYDFVDYRGTVDAFNE
                     LKSVYTLTGHPDRISLFTCDDGHGLSAPKREAALMWFRRWLDAGNSPIVREIPAAPAE
                     EDTWCTPSGQVNVMYPGEITMQEYNRRRVTELDAERRRFIEGNNREALIDTLRRLLSV
                     NSDCHEGVSIETRGSFSVKGHVAEKLIIRRTGEVPLPCHLYRNTGKGVADTLTIWLSH
                     KGKASVAADSTPDGFLPGRYRVVLAADLRGMGETAEKPQDNDPKYYNSEYHNAVLSMH
                     VGQPLPGQRVRDILMILDFISADEELGRMPLKVIASGPAALPAHLAAVLDTRIDDLEM
                     SATIESFSEITDRPVEKEWFSWIIPGGLKYFDLPDLAALRPDLKYKYTYR"
     gene            complement(4904..6511)
                     /locus_tag="PKK03_10145"
     CDS             complement(4904..6511)
                     /locus_tag="PKK03_10145"
                     /inference="COORDINATES: protein motif:HMM:NF028470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5123 domain-containing protein"
                     /protein_id="HNX84801.1"
                     /translation="MKKIIIKTGLFAGLITLLLFTSCKEQIDPVVEELDFSRAFSPVG
                     IEAVISNTTTVTLTWSSVKNTDHYLLEIYDGATATSTLVHSAEVPATEDSQVSYTYVL
                     PAGDTQFFGRIKAVSSLQGIDESKWSLVAFKTDPENLFQGYDTYMSAMNACIVQWEPE
                     ATATSLLFVSGGTQVPVTLSAEDLALGGRQVTGLANGVYEVRLMNGNFVRGRTHVIIE
                     GDVLVEPGGDLKAALDALPAGGVILLVNGVSYSLPEVDTVRTSTKVRGILPHDLPKIF
                     LATGGGNHMFDIGTAMTLSDSLVFENVDISCAYDDGDTKHRGVIDQEGDAFSIGAIKF
                     RNCIIRNSGRSAIRLRGNAAGQVINNVEFLNCVMYDFAFDSHYGVLNGAATGNFINIK
                     FLNCTVNKLRGGIINYGNGAGCQSVVIDNCTFNETTMDTGSSRYFIDFGTSNTSTGTL
                     AISDCIFGQTVDRANGVRPGSMVMTVTGSYYTSDFYDVAGPVKNLMTAYSGASTALWT
                     DPVGGNFTFLDANFEGIGLAGAPRWAD"
     gene            complement(6531..8321)
                     /locus_tag="PKK03_10150"
     CDS             complement(6531..8321)
                     /locus_tag="PKK03_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX84802.1"
                     /translation="MKKFVDILISIVFAGLLVSCEDFLEAPSQSTLDESLIFSNPTLA
                     ASAIDGIKEPIGQTNSYRGRFLTHYGANTDIEWYNNSHQAGARSDLSRYLNSAINTDM
                     NTANNAWAQMYSGIERANICIRGLRTYGNPAPGTEMGQLLGEALTLRAVYYADLVKAW
                     GDVVARFEPVSSETMYLPKSSRDEIYKQLIADLGEAAQLVAWPNGNARTTSTEGINRA
                     FVKAFRARICLAAAGYSQYPDGIRRSSDPDLSVSALYPVAMQECLDVISSGTAKLESS
                     FEGFWRKVCEENIAAGGESLWEIPFADGRGRMAFTFAVRHASADQYTEQPRGGDYGPL
                     PHVFYDYDVKDTRRDVTCVPYSWGTATNKVSKQTINTGNGVSLWYFGKLRYEWMKRYV
                     TSTNDDGLNEIYMRYAEVLLMAAEIQNELNNLGPARDYLKQIRQRAFRQADWPVKVDA
                     YLNAITTKEQMFTAIVNEQAFEYCGEMERKAALIRWNLLGTKMDEALAKMTSLSKREG
                     SYSDVPEMLYYRYAPDGESLQFYGLNRGENADMSSEYTYNTVYVSTDKLGTSKISSLY
                     TNDPDQNQFWPIWQVFIDASNGALKNDYGY"
     gene            complement(8342..>9390)
                     /locus_tag="PKK03_10155"
     CDS             complement(8342..>9390)
                     /locus_tag="PKK03_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX84803.1"
                     /translation="YRNNTKDLLIEFPVSGTGYNTQYRNMGETENKGLEIELNLIALS
                     EKKYGLSFGFNIGFNKNKILSLGVMNDFGQNTSWASTEIGNDYWIALGGSVGQMYGYL
                     NDGRYSVSDFSGYDAEEEEWILREGVVDCSNVVGTLRPGSLKLRDLSGDKIVTVADGT
                     VIGNANPKHTGGFNIEGYFYGFDVAAMFNWSVGNDIYNANKIEYTTSRYPFRNMIDVM
                     AEGKRWTNLDPATGELVNDPARLESMNATTTMWSPFMTRHVFSDWAVEDGSFLRLNTL
                     TLGYSLPEVVTDRMHIQRLRFYVSGQNVFVLTNYSGFDPEVSSRRNTVLTPGLDYSAY
                     PKSRQLLFGVNLTF"
BASE COUNT         2130 a         2617 c         2310 g         2333 t
ORIGIN      
        1 ctgaacagca ccagggcgcc ctcctcggcg tgcatcctca ggttgctttt cagtattatc
       61 ggccctgtaa gccatatgcc ccggggtatg acaacagttc ctcctccgcg ggaggatact
      121 gactctatcg ctttgcggaa tgcttcagta ttgagcacaa ctccgtctcc cacggcccca
      181 aattcagcaa tggaaactgt gttgccggca atctgaggct caattacctg tggcatggta
      241 aactcaatgt ccctgtaaat ctcgccggcg ggagaggaaa gtacctgcgg tgcaacctgt
      301 accgtgagag cggacggctt accggccttc cccggcttca gatctttcat ggcattgaat
      361 acaagctgcg caaccaccgt tgcgccggca ggctgaaaat gcgtattgtc gctgctgccg
      421 tcaggataag cttcatagag gcccggcggg aagttcatgt aataggtcat tgtgacataa
      481 tcacggcccc tgcgggaaaa ataatccatg ctcagggaat tgaggtcaat gagaccggcg
      541 ccggtctcgg cagccacctc cttcattgca tcaggatagg ccccgtgggt attgccaagc
      601 acgccatcct tccatgggta attccttgcc accggagtta ccaggacggg tatcgccccc
      661 ttctcacgtg actggctgac ataaagcctc agaaactcct tgtatcctgt cagtgaagta
      721 taccgttcac ccttgtttac cgatgcatca ttatgaccga actggatcag cacgagatca
      781 cccggccgga gctggcgata aatctctgac catcgtccct cctcaaagaa tgaacgggtg
      841 cttctcccgc ccctcgcacg gtcatcaacc actgcactgt cactcccgat aaggttcctt
      901 atcagtttca gtgagtcagg ggccatgaac gactggaaga cctgtcccca ccccatgatc
      961 ggataccgtt ttgtccggta gtccttctcc agcgagtagt cagccacggt cgaatcacca
     1021 ataagccaga cctttgttat cttcttcttc ccccctgtgc ttccgctttc agcgggaaga
     1081 ccggccataa atgccgacag tattcccgtc agaagacaca ttattaaaaa cctgccagat
     1141 ctcataaatc agtttgttgt taatattctg aaccagttga ggtcaattga acctgcatca
     1201 tttgtaaatc catcgcgcaa ggcgaagaag ccgaccttag ccccgatcca cattccctgc
     1261 ctggcaggaa aggattcacc ggcatcagta aattcgtttc cgtcaatgct gtaagagaac
     1321 cggcaactgg ccccggattc aaccgtaagc ctgaagaaca gatctccggt gggtacttcc
     1381 cgcgagagga gctctgtctc aacgcttcct gcatcagcat ccgtgcagtt cacaaccctc
     1441 aggaggagcc ggtcgtctgt ccgtcttact gatatgtacc ggtagtcacg gcccactaca
     1501 atcattccgg cctcctcgcc gacggagcgg aggttacatt ttacggaggc tgttgcggtg
     1561 aacttttccg caggcagctt tcgaaggaaa aggttgggga cctcccacat gtttcgcatg
     1621 gagtcaggac gtgcgataca gttcagcctg tagaatccgc cggcttccga ggggtatccc
     1681 caggtgaggg acggattggc gggccattgc cagttaagcg gcctgacact gctgtcaaat
     1741 tcatcaccgg tatcaattgc agcaggggag gccgtactgc caacccgtgg cgcggcaaag
     1801 taggtaacgg gctctcctgc tccgtcacca tcgtcgtcaa tgccaatcac gggccagtcg
     1861 tcaacccatt tcatcggctg gaggtgaact attctgccgt atgccccctt ctcctggaag
     1921 tggatgaacc agtcctcccc gccaggggtt gtcacccagg caccctggtg agggccatta
     1981 attggagtct tgccccggtg catcaccacc ctgtgctccc agggaccgaa taccttttgt
     2041 gccctgagcg caagctgcca gccgtttgtg actccgccgg caggggcaaa aatgtaatag
     2101 tatccgttcc gtttgtaaaa cttcggcccc tcaaccgtag gattgtcatc atggccgtca
     2161 aagatcatca ccggatcacc ggtgacaccg gtaccgtcag gcttcatcct gatgaccatc
     2221 agcacacttt tgacaccggc cctggtccca gcaaatgcgt aagccaggta agcgctcccg
     2281 tcatcatccc acagcggtgt gggatcaatc aggccatttc cctgcataac catcactggc
     2341 aggctccacg gtcccccggg acccgaagct tttaccatgt aaataccgtg gtcaggatca
     2401 gggtaaaaga tatagaactc accgttccgt tccctgatgc acggggccca tactccaccc
     2461 ccgtgcctga ccgtggaata gtattcttcg ggcagcagcc tgtcaagggc ataaccagtg
     2521 agctcccagt tgacaagatc tttggagtga agtaccggca ggcccggaac gcagttgaat
     2581 gacgaggcaa ccatatagta gtcttcgcct gcgcgaatca catccggatc agagtaatcg
     2641 gagtgaagga caggatttgt gtagactgcc ccggtgtcag tggactggct tctgttgcaa
     2701 ccggtcatga tggcagcttg aaataccagg tttatgatta ttgcgtccgc tgtcttcatt
     2761 gtattacctg taagtgtatt tgtatttcag gtctggtctc agggctgcca ggtccggcag
     2821 gtcgaaatac ttcagacctc ccggaattat ccaagaaaac cattcctttt ccacaggcct
     2881 gtcagtgatt tcggaaaatg actctattgt tgccgacatc tcaaggtcat caatacgggt
     2941 gtcaagaaca gctgcaaggt gtgcggggag ggctgccgga cctgaggcaa tcacctttaa
     3001 aggcattctt ccgagctcct catcagcaga aataaaatca aggatcatga gtatgtcgcg
     3061 tacgcgttgt cccggaaggg gctgtcccac atgcatgctc agtacggcgt tatggtactc
     3121 actgttgtaa tatttcggat cattatcctg tggcttttcg gctgtttccc ccattccgcg
     3181 cagatctgca gccagcacaa ccctgtatct gccaggcagg aacccgtccg gagtgctgtc
     3241 agcggcaact gacgcctttc ctttgtggct cagccagatg gtcagggtat cggctacacc
     3301 tttcccggtg ttccggtata aatggcaggg caggggaacc tcaccggtgc gcctgattat
     3361 cagtttttct gcgacatgac cctttactga aaaactgccc ctggtttcaa tgctgactcc
     3421 ttcgtggcaa tcactgttta ctgacagcag tcttctcagt gtgtctatga gtgcctccct
     3481 gttgtttcct tcaataaacc tccgcctctc agcatccagt tccgtcactc tcctcctgtt
     3541 atactcctgc atggtaatct caccggggta catgacatta acctgacctg acggagtgca
     3601 ccaggtatct tcttctgccg gtgcagccgg aatctcacgg acaattggtg agttgcctgc
     3661 gtcaagccat ctcctgaacc acatcagggc tgcttccctt ttaggcgccg acaggccgtg
     3721 cccgtcatca caggtaaaca gtgaaatccg gtccgggtgg ccggtcagtg tgtagactga
     3781 cttcaattcg ttgaatgcat caactgtgcc ccgataatca acgaagtcat atctccctgc
     3841 aagtataagc aatggtttgg gagcaaagat ggcagggtaa tcagctatct ccagcccggc
     3901 ttccgcctca ccggcgatct gctggcatcc gtcaccggtg ccgttgagtt cgtattccct
     3961 ctctctggtt gtgatgaagc tgcatggagc tgccgcctta accctgtcat caaaaccaat
     4021 gaaccacatt gtctgggcgc ctccgccgga attgcccatg catcctatcc ggttgctgtc
     4081 aatttccggc cgggagacga gataatcaag tgccctgata ttatcatgca attcccacgc
     4141 ggccacactg ctgccggtaa ggatagcccc tgcattgagg agagtgtgct ctgttgtgct
     4201 tcccctgaga atacgccttc cctcagaatc aataaactga accctttcac cctgtgatat
     4261 ggggtcaacc accattactg aaataccatt ggcagcaaat aaaagagctg tctccaaata
     4321 tgaaagtgtt gcctttgatg tcatctcatg cccgcagaag aagagcactg ccgggaaggg
     4381 gccctctccg gccggaaggt aaagattggc tgtaacatga tgacctggcc tcgactcaat
     4441 aataactttc tcaagcacaa tgctgccaag ggcaatacgg cctgtaatct cagggttcag
     4501 cggagtctcc ggtggaagct ctccgagaag gcggaggtaa ctcccgcggc atcctgcctt
     4561 gtattcattt atagcgcact catctgacag ggcttcacga agccgttcac ggcgaagatc
     4621 atagagggaa tgcatctcct gaagaagatg tgagcgcaag gtgaattctg tcttccagtc
     4681 ggccaccttg ttttgatcct gggctttaag cccggcagca acgacaaaaa agaaagccgt
     4741 cagtaacaat gccttgcaga ccggtatgtg gatttgcctt cgcatcatgt ctggcaggat
     4801 aattctatcc ggcggacctt atctgaaatt atcccgggga ccggggagag tacccggtcc
     4861 tccggaataa tcagagaact aacctaaaat ccaaacctga agtttagtct gcccagcgtg
     4921 gtgcgccggc caggccgata ccttcaaaat tcgcatccag aaaggtgaaa ttacctccca
     4981 cagggtcggt ccacagggca gtggaagcac cgctgtaagc tgtcatcaga ttcttgaccg
     5041 gtccggcaac atcatagaaa tcagatgtat agtatgaccc ggtaacagtc atcaccatgg
     5101 aacccggtct gaccccgttt gccctgtcaa ctgtctgtcc gaagatgcag tcactgatgg
     5161 caagtgtacc cgttgatgta ttgctcgtac cgaagtcgat aaaatacctt gagctgccgg
     5221 tgtccatggt tgtttcattg aaggtgcagt tgtcaatcac cacactctgg catcctgctc
     5281 cgttaccata gttaatgatc ccgcctctta atttattgac cgtgcaattg aggaatttga
     5341 tgttgataaa gttcccggtt gcggcaccgt tcagcacgcc gtaatggctg tcaaaggcaa
     5401 aatcatacat tacgcaattc aggaactcga cgttattgat gacctggccg gcggcattac
     5461 cgcgaaggcg gatggcgctt cggcccgagt tcctgatgat gcagtttcgg aattttattg
     5521 ccccaatcga aaatgcatca ccttcctgat ctatgacacc tctgtgtttt gtatcaccat
     5581 catcatatgc acagctgatg tcaacgttct cgaaaaccag ggagtcagac agggtcatgg
     5641 ctgtcccgat atcgaacata tggttgcctc cgccggtagc caggaatatt ttcggaaggt
     5701 catgaggcaa aattcctctc accttggtgc ttgttctcac ggtgtcaacc tcaggcaggc
     5761 tgtatgaaac tccgttgacc agcagtatca ctcctccggc aggcaatgcg tcaagcgctg
     5821 ccttgagatc accgcccggc tccaccagca cgtcgccttc aatgatgaca tgtgtccggc
     5881 cccttacaaa attcccgttc atgagacgta cctcatacac cccgtttgcc agtccggtca
     5941 cctgtctccc gccgagcgca agatcttctg cgctgagagt gaccggtacc tgggtaccac
     6001 cactgacaaa gagaagcgag gtggctgttg cctcaggttc ccattgcact atgcaggcat
     6061 tcatagccga catataggtg tcataaccct ggaacaggtt ttcaggatcg gtcttgaacg
     6121 caaccaatga ccatttcgac tcgtcaatgc cctgcaggga gctgaccgct tttatcctgc
     6181 caaagaactg agtgtcaccg gcaggtaaaa cgtatgtgta ggatacctgg ctgtcttctg
     6241 tggcagggac ctcagcagag tgcaccaatg tagaggtagc agtggctccg tcatagatct
     6301 caagaaggta gtgatcagtg tttttgactg atgaccacgt aagtgtaacc gttgtggtat
     6361 tgcttatcac ggcctctatc cctaccgggg aaaaggccct gctgaaatca agctcttcca
     6421 ccaccggatc aatctgctcc ttgcaggaag tgaacagcag cagcgtgatc agcccggcaa
     6481 atagtcctgt ctttattatt atcttcttca ttgataacag tttagttggt ttaatagcca
     6541 taatcatttt tcagggcgcc gttgctggcg tctatgaaaa cctgccagat aggccagaac
     6601 tggttctgat ccggatcatt cgtgtaaagc gagctgattt tggaagtgcc gagcttatcc
     6661 gtgctcacat agacagtatt ataggtatac tctgatgaca tgtcggcgtt ctcgcccctg
     6721 ttcagcccgt agaactggag tgattcaccg tcaggggcat acctgtagta gagcatttcc
     6781 ggcacgtctg agtagctgcc ttcccgtttg ctgaggctgg tcattttcgc cagcgcctcg
     6841 tccatcttgg ttcccagcag gttccagcgt atcagcgcag ccttgcgttc catctcaccg
     6901 cagtactcaa atgcctgttc attgacaatt gcggtaaaca tctgttcctt ggttgtgatt
     6961 gcattcaggt atgcatcaac cttaacaggc cagtctgcct gtctgaatgc tctctgcctt
     7021 atctgcttaa ggtagtccct ggccggaccc aggttgttga gctcattctg tatctcggca
     7081 gccatcagca gtacttcagc ataacgcatg taaatctcat tgagtccgtc atcatttgtg
     7141 gaggtgacat accttttcat ccactcatac ctgagcttgc cgaaatacca cagggagacg
     7201 ccgttgccgg tgttgattgt ctgtttcgat accttgtttg tggctgttcc ccatgagtag
     7261 ggcacgcagg tcacgtccct gcgggtgtcc ttcacgtcat aatcatagaa tacatgcggc
     7321 agcggaccgt aatcaccgcc acggggctgc tcggtatact ggtcagcgct agcatgcctg
     7381 acagcgaagg taaatgccat tcggccacgg ccgtcagcaa agggtatctc ccaaagtgat
     7441 tcaccgcctg cggcgatatt ttcctcacac accttccgcc agaacccttc gaaactgctt
     7501 tccagctttg cggttccgct gctgataaca tcaagacact cctgcattgc aacaggatag
     7561 agggctgata ctgacagatc aggatcactg ctccggcgta tgccgtcagg atactgggaa
     7621 taccctgctg cggcaaggca tatccgtgcc cggaaagcct ttacaaatgc cctgttgatc
     7681 ccttccgtgc ttgtggtacg ggcgttgccg ttgggccatg ccaccagttg tgcagcctct
     7741 cccaggtcag caatgagctg tttgtagatc tcatcgcggc tgctcttagg caggtacatc
     7801 gtttccgagg atacaggctc gaacctggca acaacatcac cccaggcctt tacaagatct
     7861 gcatagtaaa cggccctgag ggtaagcgcc tccccgagca gttgtcccat ctccgttccg
     7921 ggagccggat tgccataggt gcggagaccc ctgatacaga tgtttgcccg ctcaatgcca
     7981 gaatacatct gtgcccaggc attgttcgcc gtgttcatgt cagtgttgat ggcggaattg
     8041 agatatctcg acaggtctga ccttgcacct gcctggtggg agttgttata ccactctatg
     8101 tcagtgttgg ccccgtaatg tgtcaggaat cttcccctgt atgagtttgt ctgaccgatg
     8161 ggctccttta taccgtcaat ggccgaagct gcaagtgtcg gattggagaa gatcagtgac
     8221 tcatccagag ttgactgtga cggagcttcc aggaagtctt cgcacgacac aagcaggccg
     8281 gcaaaaacga tactaatcag aatatctacg aactttttca tattcatctc tttttatcgg
     8341 gttaaaaagt caggttcaca ccaaacaaca gctgcctgct ttttggataa gccgagtaat
     8401 caagtcccgg agtaagcacg gtgtttctcc tcgaagagac ctcaggatca aatcccgaat
     8461 aattggtcag cacaaacacg ttctggcccg atacgtagaa tctcaggcgc tggatgtgca
     8521 tcctgtcggt aacaacttca ggcagcgagt accccagggt cagtgtatta agcctgagga
     8581 aagagccgtc ttccaccgcc cagtcactga atacatgcct tgtcatgaag ggtgaccaca
     8641 ttgtggttgt ggcgttcatc gactccagcc tggccgggtc attgacaagc tcccctgtgg
     8701 ccgggtcaag gttggtccat ctcttccctt cagccatgac gtcaatcatg ttgcggaagg
     8761 gatatctcga tgtcgtgtac tcaatcttgt tggcgttata gatatcgttc ccgacactcc
     8821 agttaaacat ggccgcgaca tcgaatccgt agaagtatcc ctcgatatta aaaccgccag
     8881 tatgtttcgg gttggcattg ccaatcacgg tgccgtcagc aacggtcaca atcttgtctc
     8941 ccgacaggtc cctgagcttg aggctgcctg ggcggagcgt tcctacaaca ttcgaacagt
     9001 caaccacccc ttccctgagt atccattcct cctcctcggc atcatagcca ctgaagtctg
     9061 acacactgta acggccgtca ttgagataac cgtacatctg tcctaccgat ccgccaagtg
     9121 ctatccagta gtcattgccg atctccgtcg atgcccagct tgtgttctgg ccaaagtcgt
     9181 tcatgacacc aagtgacagg atcttgttct tgttaaaccc gatgttgaat ccgaagctca
     9241 gaccatattt tttctcacta agggcaatca ggttaagttc gatctcaagc cccttgtttt
     9301 cggtttcacc catattcctg tactgggtat tgtacccggt accggatacc gggaactcga
     9361 tcagcaggtc ctttgtattg ttgcgataca
//
LOCUS       DAOPXU010000112         9000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_9175_length_9000_cov_13.225601, whole genome shotgun sequence.
ACCESSION   DAOPXU010000112 DAOPXU010000000
VERSION     DAOPXU010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9000
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9175_length_9000_cov_13.225601"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1119
                     /locus_tag="PKK03_10160"
     CDS             130..1119
                     /locus_tag="PKK03_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HNX84804.1"
                     /translation="MKKIIGIGNALVDVMTIIPDETCLGQFSLPKGSMTMVDAHRSGE
                     IKQAINGMKRTLASGGSAGNTMYGLGIMGVHSSFIGKVGRDEMGNFYEKDMVDAGLTP
                     VLIRSSQSPTGTAVALVTPDSERTFATHLGAATELSADDLNISYFKGFNILYIEGYLI
                     YNLPLVEKAFQLAKENRMCVALDLASFNVVTELLPAFDRLVREYVDIVFANEEEARAF
                     TGGLNPREALDAIARKCKIAVVKTGPGGSWIKRGDEVIKVDALKVKAVDTTGAGDLYA
                     AGFLYGFSNGFGLDKCGMFGSILAGKVIEVVGARMPEEKWAEARKLVNTVAQE"
     gene            complement(1183..1833)
                     /locus_tag="PKK03_10165"
     CDS             complement(1183..1833)
                     /locus_tag="PKK03_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84805.1"
                     /translation="MFRNETLLICFTLLLVSGICSGQKTWWGEVSGFAGGGDNDIFRF
                     QELVGAGSVTGTGVWTAGADLRRLLGDHFSIGTGLSYSHQYYYTSPAPGIGGTDTPGS
                     FGLMAVPVMVRADFLKWFFADAGALLAMQTGVSRVDNMSGLGATVGAGIQYRFRSDMF
                     IRIRAYAAQYGLVHFSPENQPQTLLNGGVTLGVGYRFIRLGSCNCPDDRNPVRKFH"
     gene            complement(1844..3091)
                     /gene="fucP"
                     /locus_tag="PKK03_10170"
     CDS             complement(1844..3091)
                     /gene="fucP"
                     /locus_tag="PKK03_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HNX84806.1"
                     /translation="MTTGTLLKPTRRLVTRDFIVPFILVTSLFFLWGVAHSLLDVLNK
                     HFQDILGITKARSGLVQAALYGGYFIMAIPAGLFMNRLGYRKGIITGLLLYAAGAFLF
                     WPATFIQTFEFTLLCLFIIACGLTFLETAANPYSSVLGPKDTSEQRLTLSQSFNGLGW
                     IIGPALGGMLILGGNGDNGANKFSSMALPYMLIGVVVLVVAFMFVITRMPDIHEGEHD
                     SRGERGTFRGLFRIRHFRYAVMAQFLYVAAQTGINSFFINYVVETMPGYSPEKAAYLL
                     SLGFVFFMSGRFIGSLFMSWFSPRQMLGIYALINTAAMALVMLGLGWPSLVALYLNYF
                     CMSIMFPTIFALGLKDLGGMTKKGSSVLVMTIVGGAICPVIMGRIADLSGMAIGFVVP
                     LVCFAFIAWYAFYRAKSDKIFIK"
     gene            3266..4771
                     /locus_tag="PKK03_10175"
     CDS             3266..4771
                     /locus_tag="PKK03_10175"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNX84807.1"
                     /translation="MRTLLLTVAISLLAAFTSSAPAQQTDEQLMLKHFHSISSEEIAG
                     WMAEICSPEYNGRLAGTPEYLAAAEWMAAKFREWGIKPAGDNSSYFQWFDHPYTVVNE
                     PGELKLQIPQKDGVTIVKDYKAPADFYPGMNSGSGEVTAEVVWVGYGTTAPELNYDDY
                     KNVDVRGKIVLMNRDVPFKDVRDPDYAKWVKYCYHQYKTENAARHGAAGMLYIDGNSA
                     NPNISYIENMIVTGIGPEPLSDIFAGLGKDNKALLDQVDKTLKPASFATGKVMTMKAN
                     TTRHPEGRTCNVAGMIEGTDPGLKDEVIIIGGHLDAVGNAGGLLVAGGLDNASGVIDI
                     MAAAKALAASGIKMKRTVLFLLIGGEEVGLLGSKHYTKNPIFTREKTVTYINLDMVGN
                     GTGLAVSASESCQGLMEHFKNANEKYIHRPMKVSASRGVFYGRPRSDGLVFLADGFRT
                     MNLSTTDGVKPVYYHLPGDDATSVTPEIMEDVAKLIYLSFIDMANAETLNY"
     gene            4787..5878
                     /locus_tag="PKK03_10180"
     CDS             4787..5878
                     /locus_tag="PKK03_10180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX84808.1"
                     /translation="MAYRVLFISDISLSPFTTIADRMLIRGLRARDVDMTVVTQRPTP
                     ETQELEEEGVRVEYVIFDKKISRTIITRLRSIIDNGKIELIHVTFGKAMTNTIMAARG
                     RKLKIIGYYGSLSLHWHDPSAWLAFLSPRIDRLICASDAVEAHAKKQLPPWRRNRTVR
                     IYRGYNPEWFTGLIPVTRGEIGARDDEFLICTVGILRKVKGIRYLTEAAGLLPKEMPF
                     RIILIGDGTDSPDTLRLAAESGVPERFIHKGHQKNPPAWMAACDLYVQPSLSEGLGRA
                     ISEAMCLGKPVIVTDGGGAKELFARGDNGFVVPRGSAAELAKAITGCWKNRDSLAATG
                     EKARETILKDFHHGETVMKTFELYHELLG"
     gene            complement(5882..6466)
                     /locus_tag="PKK03_10185"
     CDS             complement(5882..6466)
                     /locus_tag="PKK03_10185"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNX84809.1"
                     /translation="MKRIFFILTVSVFAVALLPAQKKLVADAAATNIEWHGHKIVGAS
                     HTGNIDLQSGWLVLEGNSVSGGEFIVDMNSIKNSDVKDEKMRDRLVGHLKSDDFFGVE
                     KYPLSKLVITGSSKTPDGKTLFKGNLTIKEATHPVEFTATESKNGGVLTYTADINFDR
                     SLYDVRFGSGKFFSNLGDNAIVDEINLKVNLVVK"
     gene            complement(6577..7356)
                     /locus_tag="PKK03_10190"
     CDS             complement(6577..7356)
                     /locus_tag="PKK03_10190"
                     /inference="COORDINATES: protein motif:HMM:NF009386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HNX84810.1"
                     /translation="MNPKQVAIITGASRGIGRAISLALASEGYDIAAIARTSDSECML
                     SLQPEIEALGAQFFPIGLDVAHTNLHDEAISSILDRYGRIDLLINNAGIAPIERKDLL
                     DLDEETFDRVLSVNLRGPVFLARRVAREMIWLKDQVKDYRPSIMFVTSFLAYTSFTNG
                     VDVCISKAGLSMAALVFADRLSEEGINVYEIRPGLIKTELTSRSKYKFDRLITEGLIP
                     QKRWGTPEDVAKAVASLARGDWAFSAGTIFEVSGGMNIQSI"
     gene            complement(7374..8951)
                     /locus_tag="PKK03_10195"
     CDS             complement(7374..8951)
                     /locus_tag="PKK03_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84811.1"
                     /translation="MYVRTGTPAFDELIPRISRFLEQDLLELNIDGEKIRGYRSPDAR
                     SVWIRNYSDMMRALRYTEADLNSVVRHFADNQSRNGRIFDYFTTFPEKMPCERENWTK
                     YVRTPVEADTEFRFIKAAWLAWQATGDDAFIRNLMPSFERALTYVMEGRYFDREKYLF
                     RRPYTIDTWDFAYTAGSHDWLQFQIDDNTFWGYFHGDNSGYYEVFHFMSYWYSMFGDK
                     VRSALWRDRALRLRKSLNTHCFNGSFYTHFVKQIPVTIEGVDEASQLSLSNPAAINRG
                     AASPRIATAIINEYMKRRETTTAFAEWFSIDPPFPSGIFGDYKLSRGAYCNGGIMPLT
                     GGELARAALEYGFEQYGIDILMRYYDLISRNGETYLWYFPDGKPSSVETSTSPEAKPT
                     DGWGSSAMLYALVEGLAGVQDKDKLFRRVRLCPKWLSAGVEKADVKIDYKSSGAYVKY
                     AYSHTRDQLIINLSGSYEYIQLSLPVPGNYTAGDLMIGGKKKKYIMASFNQSSYLIAD
                     ADVKGECEIILDLRKKP"
BASE COUNT         2333 a         2428 c         2255 g         1984 t
ORIGIN      
        1 ggaccccctg attaacagtc aggtgctcta accaactgag ctactgagga agattctata
       61 gctccgaaaa gcgtggcaaa attaaccgct ttaaacgaat taagcaatat ctttgtcaaa
      121 attttcctga tgaagaaaat tataggcatc ggcaacgctc tcgtcgatgt aatgacaata
      181 attcctgacg aaacctgcct cggccaattc agcctcccta aaggaagcat gacaatggtc
      241 gacgcccacc gctccggcga aatcaaacag gccataaacg gcatgaaaag aaccctcgca
      301 tccggcggct cagctggcaa tacaatgtac ggcctcggca tcatgggagt ccactcatca
      361 ttcattggca aagtaggccg tgacgaaatg ggcaacttct acgaaaaaga tatggtcgac
      421 gccggcctca cccctgttct catacgctca tcacagtcac ctaccggcac cgccgtggca
      481 ctggtcacac ctgactccga acggactttc gcaacacacc tcggcgctgc aaccgaactg
      541 tcagccgatg atctcaatat cagctatttc aagggattca acatactcta catcgaaggc
      601 tacctcatct ataacctccc cctggttgaa aaggcctttc aactagcaaa agaaaaccgc
      661 atgtgcgtgg ccctcgacct ggcaagcttt aacgtggtca ccgaattgct ccctgcattc
      721 gaccggctcg tgagagaata tgtcgatata gtcttcgcca acgaggagga agcccgcgct
      781 ttcaccggcg gcctgaaccc cagggaagct ctcgacgcta tagcccggaa atgcaaaata
      841 gccgttgtca aaaccggccc cggcggatcg tggataaaga gaggtgacga agtgataaag
      901 gtcgacgccc tgaaagtcaa agcggttgac acaaccggcg ccggcgacct gtacgctgcc
      961 ggattcctgt atggcttttc aaacggattc ggcctcgata aatgcggaat gttcggatca
     1021 atcctggccg gaaaggtcat agaggtggta ggagcacgca tgccggagga gaaatgggca
     1081 gaagccagaa aacttgttaa cacagtggcc caggagtaac ccgcaaaaga tccggagctg
     1141 aagtatcgga accggcccgg cacaactcaa aatcaactga aatcagtgaa acttccttac
     1201 cggattcctg tcgtccggac agttgcaact gcccagacgg atgaacctgt aaccaacccc
     1261 gagggtcacc cctccgttaa gaagggtctg cggctgattt tcaggtgaaa aatgcaccag
     1321 cccgtactgt gccgcatagg ccctgatcct gataaacata tccgacctga accggtactg
     1381 gattccggct cctacagttg ccccaagccc gctcatattg tcaacccggg aaacacctgt
     1441 ctgcattgcc agtaaagcac ccgcatcggc aaaaaaccat ttcagaaaat cagccctcac
     1501 cataacaggc acagccataa ggccaaatga gccgggagtg tccgtgcccc ctatccccgg
     1561 cgcaggactg gtataataat actgatgtga atatgacaat cccgttccga tgctgaaatg
     1621 atcacccagc agcctgcgca ggtccgcccc agccgtccaa actcccgtcc cggtcaccga
     1681 tcccgctccg accagctcct gaaacctgaa gatatcattg tctccgccac cggcgaaacc
     1741 agatacttca ccccaccagg tcttctgtcc gctgcatatt cctgaaacta aaagcaacgt
     1801 aaaacagatc aataatgttt catttctaaa catgcgaata tgactatttt ataaatattt
     1861 tatcactctt cgccctgtaa aaggcgtacc acgcgataaa cgcgaagcag acaagaggaa
     1921 ccacaaaacc gatcgccatc cccgatagat cggcaatccg gcccattatc accgggcaga
     1981 tggcgccgcc gacaatggtc atgaccagca ccgaggaccc cttcttggtc atccctccca
     2041 gatccttcaa cccgagggca aaaattgtgg gaaacataat agacatgcaa aaataattca
     2101 ggtagagagc gacaagcgaa ggccatccga gacccagcat caccagcgcc atggctgcgg
     2161 tgttgatcag cgcatatatc cccagcatct gcctgggact gaaccagctc atgaacagcg
     2221 atccgataaa ccggcctgac atgaagaaga caaaaccaag cgagaggaga taggctgcct
     2281 tctcgggaga atatccgggc attgtctcaa caacatagtt gatgaagaag ctgtttatgc
     2341 ccgtctgtgc cgccacatac aggaactgtg ccataacagc atacctgaaa tgccttatcc
     2401 tgaacaaccc gcggaaagta cctctttcgc cgcgactgtc atgctcaccc tcatgaatgt
     2461 caggcatccg ggtgatgaca aacatgaatg caacaaccag caccaccact cctatcagca
     2521 tatatggcaa tgccatggaa gagaacttgt tggcgccatt gtcaccgttg cctccaagaa
     2581 tcagcatccc tcccagcgcc ggccctataa tccatcccag gccgttgaac gactgcgaga
     2641 gggtgagccg ctgctcagag gtgtcctttg gtccaagcac actcgagtaa ggattcgccg
     2701 cagtctccag gaaggtgagt ccgcaggcaa ttataaagag gcataacagg gtgaattcaa
     2761 atgtctggat aaatgtggcg ggccagaaga gaaaagcccc tgccgcatac agcaacagcc
     2821 cggtgatgat acccttcctg tatcccagcc tgttcatgaa gagaccggcc gggatggcca
     2881 tgataaagta accgccgtag agtgcagcct gcaccagccc tgacctcgcc ttggtaatgc
     2941 caaggatatc ctgaaaatgc ttgttcagca cgtccaggag actgtgtgca acaccccaca
     3001 ggaaaaagag gctcgtaaca agtatgaatg gcacaatgaa atcacgcgtc acaagccgcc
     3061 tggtcggttt caaaagggtc ccggtagtca ttagcaggga ttttaccatg caatatttat
     3121 cataatcctt gaaatataca acgatcgggg cagctggttt tgtatcagca atacctgttt
     3181 atcagcatgc ccaatccata tctgaaacct aaaacgatat atttgcatct gttagcggac
     3241 tattaaactt aaacggaatt acaacatgcg aacactctta ctgacagtcg ccatatcact
     3301 acttgctgcc ttcaccagct ctgcccccgc acagcagacc gatgagcagc tcatgcttaa
     3361 gcatttccat tcaatcagca gcgaagaaat agccgggtgg atggcagaga tctgctcacc
     3421 ggaatacaac ggccgcctgg caggcacccc cgaatacctg gccgcggcag agtggatggc
     3481 cgcaaagttc agggagtggg gcattaaacc cgcaggtgat aacagctctt acttccagtg
     3541 gtttgaccac ccctacactg tcgtcaacga gcccggggaa ctgaagttgc aaatcccgca
     3601 aaaggacggt gtgaccatag taaaagacta taaggcgccg gccgacttct accccggcat
     3661 gaattcaggc agcggggaag tgaccgcaga ggtggtatgg gtcggatatg gcaccacagc
     3721 ccccgaactt aactatgacg attataaaaa cgtggatgtc aggggaaaga tagtactgat
     3781 gaaccgcgac gttcccttta aggatgtccg tgatccggat tatgcaaaat gggtgaaata
     3841 ctgctatcac cagtacaaaa ctgagaatgc cgcccgtcat ggtgccgcag gcatgcttta
     3901 catcgacggc aacagcgcca atccgaacat ctcttacatt gaaaacatga ttgttacagg
     3961 catcgggccg gagcccctct cggacatctt cgccggcctg gggaaggaca acaaagccct
     4021 tcttgaccag gttgacaaaa cactcaaacc ggcatctttt gccaccggta aggttatgac
     4081 gatgaaggcg aacaccaccc gtcacccgga aggcagaacc tgcaatgtgg cgggaatgat
     4141 tgaaggaact gatcccggcc tgaaagacga ggtgattatc atcggagggc atctcgatgc
     4201 ggttggtaat gcaggcggat tgctggttgc cggaggactt gacaatgcca gcggggtgat
     4261 tgacattatg gctgccgcga aggcgctggc ggcgtcaggg ataaaaatga agcggacagt
     4321 gctcttcctg ctgatcggcg gagaagaggt tggcctgctg ggcagcaaac actacacgaa
     4381 gaatcccatt ttcaccaggg aaaaaaccgt cacatatatc aaccttgaca tggttggcaa
     4441 cggcaccggc ctggctgtaa gcgcctcaga atcatgccag ggccttatgg agcactttaa
     4501 aaatgccaat gaaaaataca ttcacagacc catgaaggta tccgcttcaa gaggagtgtt
     4561 ttacggcagg ccgcgaagcg acggacttgt attccttgct gacggcttca gaaccatgaa
     4621 cctgtcgact actgatgggg tgaaaccggt ttactatcac ctgccgggcg atgacgcaac
     4681 gtctgtcacc ccggaaatca tggaggatgt ggcgaaactc atctatctct ctttcattga
     4741 tatggctaac gccgaaacac ttaactactg aaacaaacac ctctgaatgg cataccgggt
     4801 cctcttcata agtgacatct cgctctcacc gttcaccacg atagccgaca ggatgctgat
     4861 ccggggcctc cgtgccaggg atgttgacat gaccgtggtt acccagaggc ccacacccga
     4921 aacccaagag ttggaagagg aaggggtaag ggttgaatat gtcattttcg acaaaaaaat
     4981 cagccgcacc atcatcaccc ggctgcgcag tatcattgac aacggaaaaa tagagctgat
     5041 ccatgtaacc ttcggcaagg ccatgaccaa caccattatg gctgcccggg gaagaaaact
     5101 gaaaattata ggttactacg gctcgctcag ccttcactgg catgacccat cggcatggct
     5161 ggcattcctg agcccgcgca tcgacaggct catctgtgcc agcgatgccg ttgaagccca
     5221 tgcaaaaaaa cagctcccgc catggcggag gaacaggacg gtgcgcatct accgcggata
     5281 caacccggaa tggttcactg gtctaatacc ggttacccgg ggtgagatcg gagcccgtga
     5341 tgatgagttc ctgatctgca cagtcgggat attacggaaa gtaaagggta tcaggtatct
     5401 caccgaagca gccggactgc ttccgaaaga gatgccgttc aggatcatac tgataggcga
     5461 cgggaccgat tcacctgaca ccctcaggct cgccgcggag agcggagtgc ctgagcggtt
     5521 catccacaag ggacaccaga aaaatccgcc ggcctggatg gcggcatgtg acctttatgt
     5581 gcagccgtca ctcagcgaag ggctgggcag ggcaatctca gaggcgatgt gcctcggcaa
     5641 accggtgata gtgactgacg gcggaggggc aaaagagctt ttcgccaggg gcgataacgg
     5701 cttcgtggtg ccaagaggct cggctgccga actggcaaaa gccatcaccg gttgctggaa
     5761 aaacagggac tcccttgccg ccacggggga gaaggcaagg gagaccatcc tgaaggattt
     5821 ccatcacggt gagaccgtca tgaaaacctt tgaattatac catgaactgc tggggtgaaa
     5881 attacttcac cacaagattt accttaagat tgatttcgtc aactattgcg ttgtcaccaa
     5941 ggttcgagaa aaacttgcct gaaccaaatc tcacatcata aagcgaacgg tcaaaattaa
     6001 tgtcggcggt atatgtcaag acccctccgt tctttgattc agttgcagtg aactccacag
     6061 gatgcgtggc ctccttgatt gtcaggttgc ccttaaaaag agtctttccg tcaggtgttt
     6121 tgctgctgcc ggttatgacc agctttgaca gcgggtactt ctccacgccg aagaaatcat
     6181 ccgatttcag gtgccctaca agcctgtcgc gcatcttctc gtccttcacg tcgctgtttt
     6241 tgatgctgtt catgtcaaca atgaactctc ctccggatac ggaattccct tccaggacaa
     6301 gccatccgct ctgcaggtca atgttgccgg tatgtgaagc accgactatt ttatgaccat
     6361 gccattcaat attggttgcc gccgcatccg ccacaagctt cttctgtgcc ggcagaagcg
     6421 ccacagcaaa aacagaaact gtcagtataa agaatatcct tttcattttt ttcagtgttt
     6481 agtttcaggt taaaccacgg aaaagcacat tttgttcaac aaaagttgaa tgatatcgtg
     6541 aaaaggcgaa gctcctgtgc ggtaagctgt tgattattag atactctgga tattcatccc
     6601 gccgcttacc tcgaagatgg tcccggccga gaatgcccag tcgccccgtg caagtgatgc
     6661 cacggctttt gcaacgtctt ccggggtacc ccatcttttc tggggtatga gtccctcggt
     6721 gatgagccgg tcgaatttgt atttgctccg tgaggtaagc tcggtcttta taagtccggg
     6781 tctgatctca taaacattta ttccctcctc gctgagcctg tcggcgaaga caagcgctgc
     6841 catgctcaga cctgcctttg agatgcagac atcaaccccg ttggtgaatg aggtataggc
     6901 aaggaatgat gtcacgaaca taatggaagg gcggtaatcc ttgacctgat ctttgagcca
     6961 tatcatttcc ctggctaccc gtctggcaag gaatacgggg ccccgcaggt tgacactgag
     7021 cacgcggtca aaggtctcct catcaaggtc aaggaggtct ttgcgttcta tgggagctat
     7081 gccggcgttg ttgatgagga ggtcgatcct gccgtaacgg tcaaggatgc tcgatatggc
     7141 ttcatcatgg aggttggtat gagccacgtc aagcccgatg ggaaagaact gggcaccgag
     7201 cgcctcaatc tcgggctgca gggagagcat gcattcactg tcagaggtgc gcgcgatggc
     7261 tgctatgtca taaccctccg aagccagcgc cagggagatg gccctgccga ttcccctgct
     7321 cgctccggtt ataatagcta cctgttttgg gttcatatgt cacacttttc tggtcaaggc
     7381 tttttccgga gatccaggat gatttcgcat tcacccttta catcagcatc ggcaatcagg
     7441 taactgctct ggttaaatga cgccatgatg tactttttct tcttccctcc aatcatcagg
     7501 tcacctgcgg tatagtttcc cggaacggga agagagagct ggatgtattc atagctccct
     7561 gaaaggttga tgatcagctg atcacgggta tggctgtagg catatttgac ataagccccc
     7621 gaacttttat agtctatttt cacatctgcc ttctccactc ctgccgataa ccacttgggg
     7681 cacaggcgga ccctcctgaa gagcttgtcc ttgtcctgaa cgccggccag cccttcgacg
     7741 agggcgtaga gcatggctga agatccccag ccatcggtcg gttttgcttc tggcgaggtg
     7801 ctggtctcaa ctgatgaggg cttcccgtca gggaagtacc agagataggt ttcgccgtta
     7861 cgtgagatta ggtcataata gcgcatcagt atgtcaatgc catactgttc aaatccgtat
     7921 tcaagagcag ccctggccag ctctcccccg gtgaggggca tgatgccgcc attacagtaa
     7981 gccccccttg agagcttgta gtcgccgaaa atcccggagg gaaaaggcgg atcaatggaa
     8041 aaccactcgg caaaggcggt tgtggtttca cgcctcttca tgtactcatt tatgattgcc
     8101 gtggctatgc gtggtgaggc agcgcccctg ttgatggcgg cgggattgct caggctgagc
     8161 tggctggctt cgtccactcc ctcaattgtc accgggatct gctttacgaa atgagtatag
     8221 aatgatccgt tgaagcaatg ggtgttcagg ctcttgcgaa gcctcagtgc gcggtcacgc
     8281 cagagcgccg agcgaacctt gtcgccaaac atgctgtacc agtatgacat gaagtggaac
     8341 acctcgtagt acccgctgtt gtcaccgtgg aaatatcccc agaaggtgtt atcgtctatc
     8401 tggaactgaa gccagtcatg actccctgcc gtatatgcaa aatcccaggt atcaatggta
     8461 taaggcctcc ggaaaaggta tttctccctg tcgaaatacc gtccctccat cacataggta
     8521 agggctctct caaatgaggg catcaggttg cggatgaaag cgtcatcgcc tgttgcctgc
     8581 catgccagcc aggctgcctt gataaacctg aactcggtat cagcctccac cggagtcctt
     8641 acatatttgg tccagttctc ccgctcacat ggcattttct cgggaaatgt tgtgaagtaa
     8701 tcaaagatcc ggccgttgcg cgactggttg tcagcaaagt gtcttaccac actgttcagg
     8761 tcagcctcgg tatagcgcag ggcacgcatc atgtcagaat agttcctgat ccatacggaa
     8821 cgggcatcgg gcgatctgta gcctcttatc ttctcaccat caatgttcag ctcgaggaga
     8881 tcctgttcca ggaacctgct tatcctcggg ataagctcat caaatgccgg agttccagtt
     8941 ctgacgtaca ttaccgggcc tttaggttgg ttttagtggc gttaaaatta acaattaatt
//
LOCUS       DAOPXU010000113         8830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_9428_length_8830_cov_12.156923, whole genome shotgun sequence.
ACCESSION   DAOPXU010000113 DAOPXU010000000
VERSION     DAOPXU010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9428_length_8830_cov_12.156923"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PKK03_10200"
     CDS             complement(<1..649)
                     /locus_tag="PKK03_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNX84812.1"
                     /translation="MLFIPAFKDIEAASRLISPHVHRTPVLTSKQLDAISGARLFFKC
                     ENFQKVGAFKFRGATNAVLNLTEGQRAAGVVTHSSGNHAAALAHAAVSRGVKAFIVMP
                     SSAPEVKKRAVAGYGAEITFCEPTLAAREAAAAEVIGRTGATMIHPYDNFYIIAGQGT
                     AAMELLEDIPDPDAVLAPVGGGGLLSGSAITIRHMAPPAKIYGAEPLLADDAARSL"
     gene            complement(703..1551)
                     /locus_tag="PKK03_10205"
     CDS             complement(703..1551)
                     /locus_tag="PKK03_10205"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HNX84813.1"
                     /translation="MKFLGELNYTAAEQREEAFNRAHAMSVQGVQPKLSARLNVREGR
                     MEVVDTGGRYILKPQHDYFPEMPQNEDLTMRLADLTGLNIPVHGLIWSKDNSLTYFIR
                     RFDRKGQREKIAVEDFAQLAGLTRDTKYDYSLEKVVGLVNKFCTFPAVEKTVLFKLVL
                     FGYLTGNDDMHLKNFSVITDGGITRFSPCYDLVNTTIEYNEPDVEIALPLRGRKKKLT
                     RHILVDYFGIERCELPVRSIDKVLETFVSAVPRWKELIAISFLSQGMKDKYLRLLQAR
                     MDVLGI"
     gene            complement(1629..1943)
                     /locus_tag="PKK03_10210"
     CDS             complement(1629..1943)
                     /locus_tag="PKK03_10210"
                     /inference="COORDINATES: protein motif:HMM:NF025041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HNX84814.1"
                     /translation="MRKAKVHVKGVEAGVLTEIIKGKEYTFEYVEGYNGPEVSRTVPV
                     SDRVFTFDSFPSFFEGLLPEGIQLEGLLKIRKIDRADYFSQLMAVGNDMVGAVTVEEI
                     EK"
     gene            complement(1930..2166)
                     /locus_tag="PKK03_10215"
     CDS             complement(1930..2166)
                     /locus_tag="PKK03_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX84815.1"
                     /translation="MTEQIAEIIRLHRRAARLSRIELAELAGVGKTVIYDIEKGKVSV
                     QLDTLTKILKVLNITLVLKSPLMDNLTKHVNEKS"
     gene            complement(2312..4606)
                     /locus_tag="PKK03_10220"
     CDS             complement(2312..4606)
                     /locus_tag="PKK03_10220"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNX84816.1"
                     /translation="MISSLFWIAPVAAVLALLFAWIFYRSMMKAPAGSDRMQEIAGYV
                     KEGAMAYIKRQYSVVLKVFIVLVILLFILAYLGVQNPFVPLAFLTGGFFSGLCGYLGM
                     RTATNASSRTAQGASESLNRGLKIAFRSGAVMGLIVVGFGLLDIVIWFLVLNHWVFSP
                     ENMIEGWKWAGLTFVHPGTTDHQKMIEITATMLTFGMGASTQALFARVGGGIFTKAAD
                     VGADLVGKVEAGIPEDDPRNPATIADNVGDNVGDVAGMGADLYESYAGSILATAALGA
                     ALPIVAGGDQIKAVISPMIVAAIGILLSIAGVYMVRTREQATQKNLLNALLLGTGGSS
                     ALILVAIAVMAATGWITWGVFGAVVAGLIAGVIIGQSTEYFTSDEYKPTRGIAKQTQQ
                     GPATTIIEGIAVGMQSTWIPVITIAIAIIAAYGFAGGFRDFSWGVYGIGFAAVGMLST
                     LGITLATDAFGPIADNAGGNAEMAHLPKEVRERTDALDMLGNTTAATGKGFAIGSAAL
                     TAMALVAAYMEEVKLWLGRLADGTAEGYKQIGDVLFYKSTVPAVIPEGMTAVNVSSAH
                     IKDFVGAYDLSLFNPLLIAGIFIGAMMAFLFSAMSMKAVGRAAGSMVDEVRRQFREIP
                     GIMEGTGKPDYARCVAISTKGAQREMMLPSLLAIAVPLVIGLTLGVPGVVGLLMGGMT
                     AGFTLASMLNNSGGAWDNAKKFIEKGNYGGKRSEAHKAGVVGDTVGDPFKDTSGPSLN
                     ILIKLMSMVSVVMAGLTVTWSILG"
     gene            complement(4683..7094)
                     /locus_tag="PKK03_10225"
     CDS             complement(4683..7094)
                     /locus_tag="PKK03_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNX84817.1"
                     /translation="MMRKMLVIVILAASAMLISAQDKTGYQLPPEAIVSLLDAPQTPS
                     VNLSPDNQTMLLLFRPGLPSIADLSQPELRLAGVRFDPSTNGPSRGQGISRISSMKTD
                     GTQAGDLKGLPSDASVGGVTWSRDNKWFAFTNTTDNTIELWVADAATMNARKIDEGLN
                     GILRRNITWLPDGSLIYLRTLRNRGKAPEASTVPTGPSVQESAGVRGDSPTFQDLLQT
                     VDDEKIFEYYATSELVRWDGMSAVSIGSKGMITDVSPSPDGKWFLVSITGKPFSYLVP
                     YYYFPSRTEIWDLEGKMVRLMLETPLRESLPRGYDVVIPGPRGYSWRSDRPASLMWVE
                     ALDGGDYHAKEMKYHDQVFMLEAPFTGEPAPFIATEKRFQGITWGNDNLAILMEGLSK
                     SRVRVTSTFSPASPQATLKKLWELNSDDRYNNPGFPMTVPNAWGRSVLLTTDKGRGLY
                     LSGQGASPEGDRPFVDKYDLRTGKTTRLWRSEAPFYETVNEFIDPARGLLLTVRQSVS
                     EVPNYFIRDLKKGTLKQVTKFENPYPQMAGVHKELVTYKRKDGIDLSFTLYLPAGYDR
                     AKDGPLPTILWAYPREFNDPSMAGQVSGSPYTFTRISASSVLVYVTQGYAVLNDASFP
                     IVGAEGKEPNDTFVEQLVANAEAAINKAVEMGVTDPERVAVSGHSYGAFMTAHLLSHS
                     RLFAAGIARSGAYNRSLTPFGFQSERRSFWEDPELYLQMSPFTHATKVKDPLLLIHGM
                     ADDNQGTFPIQSERYYAALKGAGAVTRLVLLPHEAHGYSARESLLHMHWEYLRWLDKY
                     VKNKK"
     gene            7216..7791
                     /locus_tag="PKK03_10230"
     CDS             7216..7791
                     /locus_tag="PKK03_10230"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNX84818.1"
                     /translation="MKSEFGTDLAGALKVLRAGGVILYPTDTVWGLGCDATNVKAVSR
                     IYEIKQRAENKSLIILVNSVAMLERYVENPPEVAIEMAELSEKPLTVVYDRGRSLAEG
                     VASSDGSVGVRVCTEPFCDAIIDALRKPLVSTSANISGSNSPAIFDEISEEVKAAVDY
                     VCLWRQDDRSRATPSSVIKVSGNGIVKILRG"
     gene            complement(7792..8487)
                     /locus_tag="PKK03_10235"
     CDS             complement(7792..8487)
                     /locus_tag="PKK03_10235"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HNX84819.1"
                     /translation="MKKVLIFSFLTIFAAQASFAQTAYTEKRQVSGFDEVAFAVSGEV
                     LINLGKDYSVELEGDRDYIKEIITEVDGDELKIRRDKWFDSGNKKVIVRITMPALDGI
                     SVSGSGRLTVNDPLRGNELDVAISGSGKAILRDVALGEVECAISGSGSLNIAGNGTIK
                     KLEISISGSGDYMGDGTRIDVLDARISGSGSCDCHVTEMLRASISGSGNINYSGDPKI
                     DASVSGSGKVRMK"
     gene            complement(8574..>8830)
                     /locus_tag="PKK03_10240"
     CDS             complement(8574..>8830)
                     /locus_tag="PKK03_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84820.1"
                     /translation="LYEGAGESGDDLMALKGFTLTDTTTGLSGIIDDFMQNPGQVIAV
                     TIIKGREVYIPLHTDLIVSVDRGQRAITMTLPVGLLSLND"
BASE COUNT         2122 a         2371 c         2151 g         2186 t
ORIGIN      
        1 ggagcgatct ggccgcatcg tcggccagca gcggctcggc cccgtagatt tttgccggcg
       61 gggccatgtg gcgtattgtt atggccgagc cgctgaggag gccgccgccg cccaccgggg
      121 ctaggaccgc gtcagggtcc ggtatatcct caaggagttc cattgccgcg gtcccctgac
      181 cggcgatgat gtaaaagttg tcatagggat gaatcatcgt cgcgccggtc cgcccaatca
      241 cctccgcggc ggcggcctca cgcgccgcca gcgtgggctc acagaaggtg atctccgcac
      301 cgtagcctgc caccgccctc ttcttcacct caggcgccga ggaaggcatc acaataaatg
      361 ccttcacccc gcgtgacact gccgcatgag ccagggcggc ggcatgattg cccgatgagt
      421 gcgtcaccac gccggcggcg cgctggcctt ccgtgagatt caggacagca ttggtggcgc
      481 ccctgaactt gaaagcccct actttctgga agttctcaca cttgaagaaa agcctcgccc
      541 cgctgatggc atcaagctgc ttcgatgtca gcaccggagt ccggtgcacg tgaggcgaga
      601 tcagcctgct ggccgcctca atatctttaa acgctggaat aaataacatc ctgatccgga
      661 attaacattg tatcaattac tattgctctg tctgagtgcc tttcagatac ccaaaacatc
      721 catccttgcc tgtaacagtc tgaggtattt atccttcatc ccctgtgaaa gaaaactgat
      781 tgctatcagt tccttccagc gaggtaccgc agaaacaaag gtttcgagta ccttatcaat
      841 gcttcttacc ggcagctcac atctttcaat tccaaagtaa tctacaagaa tatgtcgcgt
      901 aagcttcttt ttgcgtcccc tgagtggcaa agcgatctcc acatccggct cattgtactc
      961 tattgtggta tttacaagat cgtaacatgg tgaaaacctg gttattccac catcagtgat
     1021 tacagaaaag tttttgagat gcatatcatc attccctgta agatagccga ataggacaag
     1081 tttgaaaagc acagtttttt caacagccgg gaaagtgcag aatttattga ccagaccaac
     1141 aaccttttcg agactgtagt catatttcgt atcccttgtc agtccggcca gttgtgcgaa
     1201 atcttccaca gctatctttt ctctttgccc ttttctgtca aacctcctga tgaaataagt
     1261 caatgaattg tcctttgacc agattaaacc atggactggt atattgagac cagtcagatc
     1321 agcaagcctc atcgtcagat cttcattctg aggcatctcc ggaaaatagt cgtgctgagg
     1381 tttgagtatg tatctcccac ctgtatccac tacctccatc ctgccctccc tgacgttaag
     1441 ccttgcactt aacttaggct gtaccccctg aacagacata gcgtgggcac ggttaaaggc
     1501 ctcttccctc tgttctgcag cagtataatt aagctctccg aggaatttca acccggggga
     1561 taagagcttc aaccctctgc tgctgtacct gctggccccg catggctcat atgttatggg
     1621 acaccggttc atttttcgat ctcttctacc gttacagcac ccaccatatc atttcctact
     1681 gccatgagtt gtgagaaata gtcagccctg tcaatttttc tgatttttaa caacccttcc
     1741 agctggatcc cttccggcaa taatccctca aaaaatgaag ggaaactgtc aaaagtgaag
     1801 accctgtctg aaaccggaac tgtacgggat acttccggtc cattgtaacc ttccacatat
     1861 tcaaacgtgt actccttacc cttaatgatc tcagtaagga cacctgcttc gactcccttt
     1921 acatgtactt tagcttttct catttacatg ctttgtaaga ttatccatca gcggactttt
     1981 taatactaat gtaatattca aaactttgag aatttttgtg agggtatcga gctgaacaga
     2041 tacctttccc ttctctatat cataaattac agtcttacca acaccggcaa gttctgcaag
     2101 ctcaatacga cttagcctgg cagcccttcg gtgcagcctg atgatttctg caatttgctc
     2161 tgtcattacc tttttgcctc tatggcggta aatttagtgc tttcctatta aattagcaag
     2221 caattcatta attattgctg tttgagcagc aaaaaaaagg gccccggaag gccctctatt
     2281 tttttatggc atatccattc tgaccaaaat gtcagcccag tatgctccag gtcacggtca
     2341 gaccggccat aaccacggaa accattgaca tgagcttgat gaggatgttg agtgagggac
     2401 cggatgtatc cttgaacgga tctccaacgg tatcaccaac aacaccggct ttgtgagctt
     2461 cgctcctctt gccgccgtag ttgcccttct caatgaattt tttggcattg tcccatgcac
     2521 cgcccgagtt attaagcatg ctggccaggg tgaagccggc ggtcatgcct cccatgagca
     2581 ggccaacaac acccgggaca cccagggtaa ggccaatcac aaggggaacg gcaattgcaa
     2641 gcagtgacgg aagcatcatc tcgcgttgtg cacccttggt tgatattgca acacaccttg
     2701 catagtcagg tttgcctgtg ccttccatga tacccggtat ctcgcggaac tggcgcctaa
     2761 cctcatcaac catgctgcca gcagcgcggc ccacagcctt cattgacatt gcgctgaaga
     2821 ggaatgccat catggcaccg atgaaaatac cggcgatgag aagcggattg aacagtgaca
     2881 ggtcatacgc acccacaaag tccttaatat gagcggaact tacgttcacc gcggtcatcc
     2941 cttcaggtat tacagcaggc acagtgctct tatagaacag cacatcacct atctgcttgt
     3001 aaccctcggc ggttccatcg gcaagacgac caagccagag cttcacttct tccatgtatg
     3061 ctgccacaag agccatggct gtaagagccg ccgaaccgat tgcaaatcct tttccggttg
     3121 ctgcggtggt attgcccagc atatccaggg cgtcagtgcg ctcacgaacc tccttgggta
     3181 gatgagccat ctcggcgtta ccgcctgcat tgtcagcaat aggaccgaag gcgtcagttg
     3241 ccagggtgat tcccagtgtt gagagcatac ccacggcagc aaatccgatg ccatatacac
     3301 cccacgagaa gtcccggaat ccgcctgcaa agccatatgc cgcaatgatt gcaatggcaa
     3361 ttgtaatgac agggatccag gtagactgca ttccaacggc aataccttca atgatggtgg
     3421 ttgccggacc ctgctgtgtc tgttttgcaa tgcccctggt gggcttatac tcatcagatg
     3481 tgaagtattc cgttgactgt ccgatgataa caccggcgat aagaccagca acaacggcac
     3541 caaacacacc ccatgtaatc catcctgtgg cagccatgac ggcgattgcc accagtatca
     3601 gggccgagct gcccccggta ccgagaagaa gtgcattgag caggttcttc tgtgtagcct
     3661 gctccctggt gcgtaccatg taaacaccgg ctattgagag cagaattcct attgcagcaa
     3721 cgatcattgg cgatatgaca gccttgatct ggtcaccacc ggcaacaatc ggcagtgctg
     3781 ctccgagagc tgctgtagcc agtatcgagc ctgcatatga ttcgtaaagg tcagcgccca
     3841 tacctgccac gtcacctacg ttgtcaccaa cgttgtcagc aatggttgca gggttacgcg
     3901 ggtcatcctc ggggatgcct gcctcaacct taccgaccag gtcagcgccc acgtcagcag
     3961 ccttggtgaa gatacctccg cccacgcggg caaagagagc ctgcgttgag gcgcccattc
     4021 cgaatgtaag catcgttgca gtgatctcga tcatcttctg atgatcagtg gtaccaggat
     4081 gaacaaaagt cagaccggcc catttccatc cttctatcat attctcaggg ctgaataccc
     4141 aatggttaag gaccaggaac cagattacaa tatcaagtaa tccgaagccg actacaatca
     4201 gacccattac ggcaccgctg cggaaggcaa tcttaagccc tctgttgagt gattcggaag
     4261 ctccctgtgc tgtccttgag gaagcgttgg ttgcggttct cattcccaga tacccgcaga
     4321 gacccgagaa gaatccgcct gtcaggaatg ccagtggcac aaaggggttc tgaaccccaa
     4381 ggtaagccag gataaaaagg agtattacaa gcacaataaa gaccttgagc acaacgctgt
     4441 actggcgttt gatataggcc atcgcacctt ctttgacata accggcaatt tcctgcatcc
     4501 ggtcgctgcc ggcaggtgcc ttcatcatgc tcctgtagaa aatccatgca aagagcaatg
     4561 ccagcaccgc tgcaacaggt gctatccaga acaaactact gatcataagc ttaggttttt
     4621 ataagatttg aggtacaaaa ctaatcagtt tttttacata acctaaccgc acccggcagt
     4681 tatcattttt tgtttttcac atatttatca agccatctga ggtattccca gtgcatatgg
     4741 aggagtgact cccgggccga gtatccgtgt gcctcatgag gcagcaggac cagtcttgtc
     4801 acagcgccgg ctcccttgag tgccgcatag tacctctcgc tctgtatcgg gaaggtaccc
     4861 tggttgtcat cagccatgcc gtggatcagc agcagcggat ctttcacctt tgttgcatgg
     4921 gtgaagggtg acatctgaag atataattca ggatcctccc agaaactgcg gcgctcgctc
     4981 tggaacccga acggtgtcag tgaacggtta taggcaccgc tgcgggcaat gcctgccgca
     5041 aagagtctcg agtgcgacag caggtgtgcc gtcatgaaag caccatacga gtggccgctg
     5101 actgcaaccc gctccggatc agtgacaccc atctcaacag ccttgttgat tgctgcctcg
     5161 gcattggcaa caagttgttc cacaaatgtg tcattgggtt cctttccctc cgcacccaca
     5221 atggggaagc tggcatcgtt caacacggcg tacccctggg tcacataaac aagcaccgat
     5281 gaggcgctga tgcgtgtgaa ggtatagggg gagccactca cctggcctgc catcgaggga
     5341 tcattgaact cacgcggata agcccacagt atagtcggaa gcggaccgtc cttggccctg
     5401 tcatacccgg ccgggaggta gagggtgaat gaaagatcaa taccatcctt tcgcttgtag
     5461 gtgaccagct ccttatgtac acctgccatc tgcggatagg gattctcaaa tttcgttacc
     5521 tgcttcaggg ttcctttctt caggtcacgt atgaaataat tcggtacttc agagacggat
     5581 tgcctcacag tcagaagaag gcctcgggca ggatcaataa actcatttac cgtctcataa
     5641 aaaggagcct cagaacgcca cagcctggtt gtttttccgg tcctgagatc atatttgtct
     5701 acaaaaggcc tgtcgccctc cggtgacgca ccctgacccg agaggtacaa gcccctgccc
     5761 ttatcggtag tcagcaatac tgaccttccc catgcattgg gtactgtcat cgggaacccg
     5821 gggttattgt acctgtcgtc agagttgagc tcccagagct tcttaagagt ggcctgcggg
     5881 ctggcgggac tgaatgttga tgtaaccctg acacgtgact ttgacaatcc ttccatcagg
     5941 atagccaggt tatcattgcc ccaggtaatt ccctggaacc gtttttccgt ggcaatgaaa
     6001 ggtgccggtt cgcctgtaaa tggtgcctcg agcatgaaaa cctggtcatg gtacttcatc
     6061 tcctttgcgt ggtaatcacc tccgtcaagg gcttccaccc acatcagtga tgccggcctg
     6121 tcactgcgcc agctgtatcc ccttggacca ggtatgacaa catcatatcc cctgggcagg
     6181 ctttcccgga gcggagtctc gagcataagc cttaccatct ttccttcaag gtcccatatc
     6241 tctgtccgtg acgggaaata gtaatacgga accaggtacg aaaatggctt tccggttatg
     6301 gaaacgagga accactttcc gtcaggtgag ggtgagacat cagtgatcat tcccttgctg
     6361 ccgatggaaa ctgctgacat gccatcccat ctgaccagct ctgaggtggc ataatattca
     6421 aatatctttt catcatcaac agtctgcagg aggtcctgga atgtgggtga atctcccctg
     6481 actcctgcgc tttcctgaac cgaaggaccc gttggcacgg tggaggcttc gggggctttt
     6541 cccctgttgc gcagggtgcg cagatatatc agcgaaccgt caggcagcca tgtaatgttc
     6601 ctgcgcaata tgccgttcag tccttcatcg atttttcgtg cattcattgt tgcagcatcg
     6661 gcaacccaca gttcaatcgt attgtcagtg gtgttggtga aggcaaacca tttgttgtcg
     6721 cgtgaccatg taactcctcc cacactggcg tcggagggaa gtcccttcag atctccggcc
     6781 tgcgttccgt cagtcttcat cgaagagatc cggcttattc cctgcccgcg gctcggaccg
     6841 ttggtcgaag gatcgaacct gacccctgcc aggcgcagtt caggctgcga gaggtcggca
     6901 atagatggta atcccggacg aaataacaac agcatcgtct ggttgtcagg gctgagattc
     6961 acagacggcg tctgcggggc atccaggagt gagacaatag cttcgggagg aagctgatag
     7021 ccggttttat cctgtgcgct gatcagcatt gctgaagcgg caaggatgac aatgacaagc
     7081 attttacgca tcataatggt tctttttggt ctgtctcaaa tttaaccttc tgaattcagg
     7141 tagagaaacc aaataagagc tattattgtt ttttaacttt ataaaaaact gtctatgacc
     7201 gttcataata aaggaatgaa gagtgaattc ggaaccgacc tggccggtgc cctgaaggtt
     7261 ctcagggccg gcggggtaat actttacccc actgacactg tctgggggct gggctgtgat
     7321 gccacgaacg tgaaagccgt aagccggatc tatgaaatta agcagagggc tgagaacaag
     7381 agccttataa tacttgtgaa ctccgttgcg atgcttgaga ggtatgttga aaatccgcct
     7441 gaggtggcca tagagatggc agagctctcg gagaagccgc tgacggtggt ctatgacagg
     7501 ggaaggagcc tggctgaggg ggttgcctct tctgacgggt cagtgggtgt gcgtgtatgc
     7561 acggaaccgt tctgcgatgc gatcattgat gcgctcagga agccgctggt atcaacatcg
     7621 gcaaacataa gcggcagcaa cagccctgca atatttgacg agataagtga agaggttaag
     7681 gctgcggttg attacgtatg cctctggaga caggatgacc gaagcagggc cacaccctct
     7741 tcggtcatca aagtatccgg aaacggtatt gtaaagattc ttcgcggatg attacttcat
     7801 ccgcaccttg cctgacccgg aaacagaggc atcaatcttc ggatcacccg agtaattgat
     7861 attgccgctg cccgagatag aggctctcag catttcagtc acatggcagt cgcaactgcc
     7921 gctgccggaa atcctggcat ccaggacatc aatcctggtg ccatccccca tatagtcacc
     7981 ggaacctgaa attgagattt caagtttctt aatggtgccg ttcccggcga tgttaagact
     8041 gcctgacccg gatatggcgc actcgacttc cccgagtgcc acatcacgga ggattgcctt
     8101 gccgctaccg ctgatggcca catcgagctc attccccctg agaggatcgt ttaccgttaa
     8161 cctccctgaa cccgaaacgc ttataccatc gagggcaggc atggtgattc ttacaataac
     8221 ctttttgttc ccggaatcaa accatttatc ccgtctgatt ttcagctcat caccgtcaac
     8281 ttcggtaatg atctctttga tgtaatccct gtcaccttcc agctctacac tgtaatcctt
     8341 gccgaggttg atcagcactt cgcctgaaac ggcgaaggcg acttcgtcga agcctgacac
     8401 ctgtctcttt tcggtatagg ctgtctgagc aaatgaagcc tgggcggcga atatcgttaa
     8461 gaatgagaaa atcaggactt ttttcatagc tgctccgttt tatttagtta taacagaaag
     8521 acggtgcaga atgttctttg ctgcacagtg aataaaaaaa aggggcagga atgtcagtcg
     8581 ttaaggctca acagacctac agggagggtc attgtgattg ccctttgccc gcggtcaaca
     8641 gacacaatca ggtcagtgtg gagggggatg tatacttccc ggcctttgat gatggtcacg
     8701 gcaataacct ggccgggatt ctgcataaaa tcatctatta tgcctgacaa tccggttgtt
     8761 gtgtcggtca gtgtgaatcc cttcagcgcc atgaggtcat cacctgattc gccggcgcct
     8821 tcgtagagca
//
LOCUS       DAOPXU010000114         8639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_9706_length_8639_cov_11.949790, whole genome shotgun sequence.
ACCESSION   DAOPXU010000114 DAOPXU010000000
VERSION     DAOPXU010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8639
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9706_length_8639_cov_11.949790"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1665)
                     /locus_tag="PKK03_10245"
     CDS             complement(97..1665)
                     /locus_tag="PKK03_10245"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNX84821.1"
                     /translation="MKIRIEKIRMMLVAVILFAVVMPAFTQEAASDLEMVYKIRQEGQ
                     RNSDIEKLAYIMTDLAGPRLTGSAGIDRANEIARAKLAEYGLSNVRIEEVSDFNRGGW
                     DLTKAYAAMVAPYYNNFSVTPVAWTAGTNGLIRGEVILVDIKTLADIEKYRGKLNGKI
                     IMTPTTTQYEVSFEPLASRYTDEELENLAKADAGGRAARRPVTGDFMAMRELRTKVNE
                     FLKSENILMSVSQGSAFNIPRSSGSSYDGKGTPPVTEISIPMEAYGRMERMLKGGEKV
                     EMEAEIKAEFTPNRKVYNVIGEIPGTDPKLKDQVVLLGAHIDAWHGGTGAADNASGCI
                     VMMEALRILKALDVKPRRTIRVALWGGEEQGLYGSRGYVSKYLYDREKNEKKPGFDKF
                     SVYFNMDNGTGRYRGVYLQENILARSFFEKCMEPVKDMGFTTITSRNTGGTDHQSFDG
                     AGLPGFQFIQDEIEYGRGYHTLADTRERLLVPDLRHNAIITAWLAWNASQQEQLLPRK
                     PEMKSNQQRSPYGF"
     gene            1767..4244
                     /locus_tag="PKK03_10250"
     CDS             1767..4244
                     /locus_tag="PKK03_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNX84822.1"
                     /translation="MIYPDTYELKIGFDRIREQLNGCCISNAGIRRVEDMHPSFAADE
                     VRDRLSLTAEFQMILAFGEQFPSDNYNDPFATLEKLRVEGTFPEIEEVVALRGSLDTI
                     RRIIGFFRNRKDGRYPLLTGLTGKVVYYPFVSEAVDRIIDKEGKIRDGASPALKTIRA
                     ELASKNLAAVRKLHAVLRQAQADGIIDRDVTVAVRNGRGVIPVSASNKRGIKGFVHDE
                     SATGKTVFIEPSEVVELNNEITELEHQEKREIVRILTALADTIRPYIDELEENCSFLA
                     EIDFLRAKAIFGNRLSCIMPSVADTPHIRWINARHPLLTLSFARTAGREVVPLTITID
                     SRERILLISGPNAGGKSVCLKTVALIQYMLQCGMTIPAGEGTEAGVFGKFFIDIGDEQ
                     SIENDLSTYSSHLINMKQMLRHADDSSLVMIDEFGTGTEPVLGGALAEAILAELNRRG
                     AYGVITTHYTNLKHFATSQEGISNGAMAFDNQLMQPLFRLDQGKPGSSFAFEIAKKIG
                     LPQEILADAATRAGEGNVDFDRNLREIARDKRYWEKKREAVRRHEKRLEELIATYETQ
                     LGDIKTQKKEIITQAKSKAEEMLRDTNRMIENTIRTIREAQAEKEKTKEVREELDSFR
                     EHLDREEKQVEIPVPPKPRIDTPAASIVKRISAKKETPQPVKKKKGPLAPGDYVKVSG
                     TDTVAQVTGIRGNKVQIISGNLMMNVDISMTEPVSEEEQKKATLPGTRQQKVDWSLTT
                     RRNQFNPEIDVRGMRTGEALQVVQDFVDTALMIQFRNLRILHGKGNGILRQMIRQYLE
                     STGAVKRATDEHVDRGGAGITLVELDI"
     gene            4310..6238
                     /locus_tag="PKK03_10255"
     CDS             4310..6238
                     /locus_tag="PKK03_10255"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HNX84823.1"
                     /translation="MKKLTLLFSALVMLMTLPANGQTNDFYDDAPTMQLKGAKKILVT
                     GEIEKDVTVDLSRLQKHTITVKETTITDGNVGFTGAYRYDGYSVYDILDRAVLKKKNE
                     AEFPPIIDLYVEITNDRGETAVFSWGEIYYPVNMHRLMIATSVARIVPSKAKDLWPLP
                     AETKVVAGNDLVTSRNISNPVKITVKSLNSKYPINKGMNPMFSETMKLCVNGKQRAIL
                     SELPEGMDSETYHTVFYGRGMGIHGTTPFTGAMLNELIRDYYRLSPEALQKGMVVIAG
                     LDGYRAAFTLSEIVNRNDQQEVLIIDRDNYEGAGRFSIFPACDFFSDRAIKAVMEVNL
                     LLPTAGSPWAIVVHGGAGAITRDRMNAEAEAEYRRALQAVIDSGAAVLSAGGSALDAV
                     ERAIRMMEDSPLFNAGKGAVFTHEGRNELDASVMDGSNLAAGAVAGVTDIRNPITAAR
                     TVMEKSPHVMLTGRGASEFAAEQGLEIVDPLYFREESRFRELQRALEREKHGTVGCVA
                     LDMNGDLAAGTSTGGMTNKRYNRVGDAPVIGAGTYASNATCAVSATGHGEFFIRYTVA
                     HDISALMQYRGLSLDEAAREVIKNKLVKAGGTGGIISVDGKGNISMPFNTGGMYRGYK
                     NSDGKKGVFIFADEPDRL"
     gene            complement(6299..7381)
                     /locus_tag="PKK03_10260"
     CDS             complement(6299..7381)
                     /locus_tag="PKK03_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84824.1"
                     /translation="MKKLFTNQRVTLFAALLCSVALSASAEEVKKEFHRELTPGDNST
                     VTVINKFGSVVTETWDKNNVVVDVTVKVEHPSADRARKLLDMIRVEFTEEGGNLKAET
                     IFSDDFSSISWKGSNNNFSINYVIKMPARVNFDVTNKYGNTVIDELSGLVTANVKYGD
                     LTVDKLTRGNVKPLNTLIVAYGKATVYELGWAEINARYVGMFSVERATALLVDSKYSK
                     VSVKKVSSLVADSKYDGYSVGEANNIVVLGGYTDLRFDKVSRKLDVETKYGNLSVNYV
                     PAGFERLAVKAGYCAVRLGIDPSACYRLDAKSSYGSIKLDDSLFSADRRIVGNTSTEM
                     AGKVGKCSTPSSEVTIDTSYGSVRLN"
     gene            complement(7362..7925)
                     /locus_tag="PKK03_10265"
     CDS             complement(7362..7925)
                     /locus_tag="PKK03_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84825.1"
                     /translation="MNMIDDIIRKSRSEFDDREPSDGHFERFSFKLATRLHAGKAKRS
                     IIPYLLKAAVVTLLVTLSSLWTFDHFVRPNLKTKMTLSQVSPEYREVEKYYVQQVNLM
                     ENELTLIDMSNPEQKEALMVEMASMDSVYVELQKELRANPDDQRIIDAMIKHYQTKIE
                     VMSYILDQLKEIKAETVKPVSHEEVVY"
     gene            complement(7925..8497)
                     /locus_tag="PKK03_10270"
     CDS             complement(7925..8497)
                     /locus_tag="PKK03_10270"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX84826.1"
                     /translation="MENVEAAFRNIHQDLIDGCRSGDQRAQFQVYKLYYKAMYNTSLR
                     IVNDEMEAEDVMQEAFLSAFEKINTYSGTVSFGAWLKRIVVNRSLDVLGKRKAIFEDI
                     ESHAGIRDDSGEETAYHEDLALRVEEVKAAIERLPEGYRVILSLYLIEGYDHDEIAEM
                     LNISSSTSRSQLSRAKQKLVGELKNRKIYE"
BASE COUNT         2122 a         2351 c         2167 g         1999 t
ORIGIN      
        1 ttcaaacaga atagaaaaac aatgagttgc agttggcctg tggtcagcgc cgggtgaacc
       61 atacccgttg cctgtgactt aaggcctgcg cagggatcag aacccgtagg gtgaccgctg
      121 ctggtttgat ttcatctcgg gcttgcgggg cagcagctgt tcctgctgtg atgcattcca
      181 cgccagccat gccgttatga ttgcattatg cctcaggtca ggaaccagga gacgttcacg
      241 ggtgtcggca agtgtatgat agccacggcc atattcaatc tcgtcctgaa tgaactggaa
      301 tccggggaga cctgcgccgt caaagctctg atggtcggtg ccaccggtgt tcctgcttgt
      361 tattgtggtg aatcccatgt ctttgaccgg ttccatgcac ttctcgaaaa atgaccttgc
      421 aaggatgttc tcctgaagat agacaccgcg gtaacgaccg gtaccattat ccatgttgaa
      481 gtatacgctg aacttgtcaa atccgggttt cttctcgttc ttttccctgt catacaggta
      541 ctttgagaca tatccccttg atccgtaaag accctgttcc tcaccacccc agagagcaac
      601 acggatggtt ctgcgcggtt tgacatcaag ggccttcagt atgcgaagcg cttccatcat
      661 cacgatgcag cccgatgcgt tgtctgcagc tcccgtgccg ccatgccagg cgtcgatatg
      721 cgcacccaga agaacaacct ggtccttcag tttgggatcg gtgcccggaa tctcgccaat
      781 gacattgtag actttcctgt tgggagtgaa ttcagccttt atctcagcct ccatctcaac
      841 cttctcaccg cccttcagca tcctctccat tctgccatag gcttccatcg ggatgcttat
      901 ctcagtcacc ggaggtgtgc ccttgccatc ataggatgac cctgacgacc tggggatgtt
      961 aaaggcactt ccctgtgaaa ccgacatcag aatattctca ctcttcagaa actcattcac
     1021 cttggtgcgc agctcacgca tcgccataaa gtcgcctgtt accggccttc ttgccgcacg
     1081 tcctccggca tcagcctttg caagattctc cagctcctca tcggtatacc tggatgccag
     1141 cggctcaaag ctgacctcat attgtgtagt ggtgggtgtc atgatgatct tgccattcag
     1201 cttgccacgg tacttttcga tgtcagcgag ggttttgatg tcaacaagaa ttacttcgcc
     1261 cctgatcagg ccgtttgtcc cggccgtcca cgccaccggc gtaacggaaa agttgttgta
     1321 gtatggagct accatagctg catacgcttt tgtcaggtcc catccaccac gattgaaatc
     1381 actcacttcc tcaattcgta cgtttgacaa accatattct gccagcttgg cccgcgcaat
     1441 ctcattagcc cggtcaatgc cggctgatcc tgtgagcctg ggcccggcaa ggtcagtcat
     1501 gatataggca agcttctcga tgtcagaatt acgctgaccc tcctgcctta tcttgtatac
     1561 catttcaagg tcactggcag cttcctgtgt gaaagccggc atgaccacag caaacaggat
     1621 gactgcaaca agcatcatcc tgattttttc aatacggatt ttcattttat tttgtggtta
     1681 acgggattaa agataaattc agaagtttta cacccgtgca gtgttaacat taattaacgt
     1741 tacttttgcg aggagatgac caccagatga tctatcccga cacatatgaa ctgaagatcg
     1801 gctttgaccg tatcagggaa cagcttaacg gatgctgcat cagcaatgcc ggaatccgca
     1861 gggttgaaga catgcacccg tcatttgctg cagatgaagt cagggacagg ctttccctta
     1921 cggccgagtt ccagatgatc cttgccttcg gcgaacaatt cccctctgac aactacaatg
     1981 atccctttgc aaccctggaa aaactccgcg tggagggcac ttttcctgag attgaagagg
     2041 tggttgccct gcggggatca cttgatacca tacggcgaat cataggcttc ttccggaaca
     2101 ggaaggacgg acgatacccg ctgctgaccg ggctgaccgg gaaagtggta tactacccgt
     2161 ttgtttcaga ggctgttgac cgtattattg acaaagaggg taagatacgt gacggcgctt
     2221 caccggctct taaaacgata cgcgccgaac tggcttcgaa gaaccttgcg gcagtgagaa
     2281 aactgcacgc agtgctgcgc caggcccagg ctgacggcat cattgaccgc gatgtgaccg
     2341 tggcagtgcg caacggcaga ggcgtgatac ccgtaagcgc atcaaacaaa agaggaataa
     2401 aaggattcgt tcatgatgag tctgccaccg ggaaaaccgt tttcatagag ccttcagaag
     2461 ttgttgaact caacaacgag atcaccgagc tcgagcacca ggagaagcgc gagatcgttc
     2521 gtatccttac agccctggct gacaccatca gaccatacat cgatgaactg gaggagaact
     2581 gcagcttcct ggcggagatc gactttctca gggcaaaagc catcttcggc aacaggctca
     2641 gctgcatcat gccttcggtg gctgataccc cgcacataag atggatcaac gcccgccacc
     2701 cgctgctgac actctctttt gcccgcacag cgggacggga ggtggtgcca cttacaatta
     2761 ccatcgacag cagggaaaga attcttctca tctccggccc caacgccgga ggaaaatcag
     2821 tctgcctcaa gaccgtggca ctcattcagt atatgctcca gtgcggaatg acaatcccgg
     2881 caggcgaggg caccgaggct ggcgtgttcg gcaagttctt tatcgatatt ggtgacgagc
     2941 agagcattga aaatgacctc agtacctaca gctcacacct catcaacatg aaacagatgc
     3001 tcaggcatgc tgatgactcc tcactggtca tgatcgatga gtttggcaca gggactgaac
     3061 cggttctcgg cggagccctc gcagaggcaa tccttgccga actcaacaga aggggcgctt
     3121 acggtgtaat taccacccac tatacaaacc tcaagcactt cgctacatca caggagggaa
     3181 tcagcaacgg ggcaatggcc tttgacaacc agcttatgca gcccctcttc cgccttgacc
     3241 agggaaagcc gggaagctca ttcgcctttg agatagccaa aaagataggc ctgccacagg
     3301 aaatactggc agatgccgca accagggcag gagaggggaa cgtcgacttc gacaggaacc
     3361 ttcgcgagat tgcccgtgac aaacgctact gggagaagaa gcgcgaggcg gtacgccgcc
     3421 acgagaaacg gctcgaggag ctgattgcaa cctacgagac acagctcggc gacataaaga
     3481 ctcagaagaa ggagatcatc acccaggcga agagcaaggc ggaggagatg cttcgcgaca
     3541 ccaaccggat gatagaaaac accatccgga ccatccgcga ggcacaggcc gaaaaggaga
     3601 aaacgaaaga ggttcgcgag gagcttgact cattccgtga acaccttgac agggaagaga
     3661 aacaggttga gatacccgtt ccgcctaaac cccgcattga cacccctgcc gccagcatag
     3721 taaagaggat ctcagcaaag aaggagactc cgcagccggt taaaaagaag aaaggaccac
     3781 tggcgccggg cgactatgta aaggtatccg gcaccgatac cgtggcccag gtgaccggga
     3841 tacgcggaaa caaagtacag atcatctccg gaaacctgat gatgaatgtt gatatcagca
     3901 tgactgaacc ggtcagcgaa gaggaacaga aaaaggcaac actgccaggc acccggcagc
     3961 agaaggtcga ctggtcactc acaacccgcc gcaaccagtt caatcctgag attgatgttc
     4021 gtggcatgcg caccggggag gccctgcagg tggtgcagga tttcgtagat accgccctga
     4081 tgatacagtt caggaacctg aggatactgc acggcaaggg taatggcatc ctgagacaga
     4141 tgatcaggca gtacctggaa tcaaccgggg cagtaaagag ggcaacagat gaacatgtag
     4201 accggggcgg ggcaggcatc accctggtgg agctggatat ttgacctgct ttttatcatt
     4261 ttgtccgcaa cgcgaattga atagatttgc ataacgtaaa acctgaatca tgaagaaact
     4321 gacactgctt ttttccgccc tggtgatgct gatgacgctt ccggccaacg ggcagaccaa
     4381 tgatttttat gatgatgcgc ccacaatgca gctgaagggc gccaaaaaga tccttgtcac
     4441 cggcgagatt gagaaggatg tgacagttga cctgtcacgc ctgcagaaac acaccatcac
     4501 tgtaaaggag acaacaatca ctgacggaaa tgtcggcttc acaggagcat accgttatga
     4561 cggctattca gtatatgata ttcttgacag ggcagtcctg aagaaaaaga acgaagcaga
     4621 attccctccg atcattgacc tctatgttga gatcaccaat gacaggggcg aaaccgcagt
     4681 attctcatgg ggagaaatct actacccggt aaacatgcac cggctgatga ttgccacttc
     4741 cgttgcccgc atcgtgccct ccaaggcaaa ggacctatgg ccattgccag cagaaacgaa
     4801 ggttgtggcc ggcaatgacc tggtcacatc acgcaatatc agcaacccgg tgaaaatcac
     4861 cgtgaagtca ctcaacagca agtatcccat taacaagggg atgaacccga tgttttcaga
     4921 gacaatgaag ctatgcgtca acggaaagca gcgtgccata ctgtctgagc ttcccgaagg
     4981 catggacagt gagacctatc atacagtgtt ctacggacgc gggatgggca ttcacggtac
     5041 cacacccttt accggtgcaa tgctgaatga actgatcagg gattattaca ggctctctcc
     5101 cgaagcgctt cagaagggga tggtggtcat cgccggcctc gacggttacc gtgcggcatt
     5161 cactctgagc gagatagtta accgtaatga ccagcaggag gtgctgatca ttgaccgtga
     5221 taactacgag ggtgccggcc gcttctccat cttcccggca tgcgatttct tctctgaccg
     5281 cgccatcaag gcagtgatgg aggtcaatct tcttttgccg acagcagggt ctccgtgggc
     5341 cattgtggtg catggcggcg ccggcgccat tacccgtgac cggatgaatg ccgaggcaga
     5401 ggctgaatac cgcagggcgc tgcaggcagt aatcgacagc ggcgccgctg tgctcagcgc
     5461 cggcggctcg gccctcgacg ccgtcgagcg tgccatccgg atgatggaag actcacctct
     5521 cttcaacgcg ggcaagggcg ctgtattcac ccacgagggc cggaatgaac tcgacgcctc
     5581 cgtcatggac ggaagcaacc tcgccgccgg cgccgtagcc ggagtcacag acatccgcaa
     5641 ccccatcacc gccgcacgca ctgtaatgga gaaatcacca cacgtcatgc tgacaggcag
     5701 gggagcatct gaatttgcag ctgaacaggg cctcgagata gtcgacccat tatacttccg
     5761 cgaggaatca cgattcaggg aactgcaacg cgctcttgaa agagaaaaac acggcactgt
     5821 gggctgcgtg gcccttgaca tgaacggcga cctggcagca ggcacttcca cagggggtat
     5881 gacaaacaaa agatataacc gcgtcggaga tgcaccggtg attggagcag gaacctatgc
     5941 cagcaacgcc acatgcgccg tatccgcaac aggccacggt gagttcttta tcaggtatac
     6001 cgttgcccat gatatctctg cactgatgca gtacaggggc ctctcgctcg atgaagcagc
     6061 ccgcgaggtg atcaaaaaca aactggtgaa ggccggcgga actggaggaa tcatctctgt
     6121 cgacggcaag ggcaacataa gcatgccatt caataccgga ggaatgtatc gcgggtataa
     6181 gaattcagat ggcaaaaagg gcgtcttcat tttcgccgac gaacctgaca ggctttaatg
     6241 acaaaaaaaa acgcggcgcc ctgtcactgg cgccgcgatt cttttttgct gtccctgctt
     6301 agttcagcct gactgaaccg taggatgtat cgatggtaac ctctgatgac ggcgtgctgc
     6361 atttgcccac cttgcctgcc atctcggttg atgtgttgcc aacaatccgc ctgtcggccg
     6421 agaaaagact gtcatcaagc ttgatgcttc cgtatgagct tttggcatcg agcctgtagc
     6481 aggccgaagg atcaatcccg agcctgacgg cacagtaacc tgccttgacc gctaacctct
     6541 caaatcctgc aggcacatag ttaactgaaa gatttccgta ttttgtctcc acatcaagct
     6601 tcctgctgac cttgtcaaac ctgaggtccg tatagccgcc gagaaccact atgttgttgg
     6661 cctcgccaac cgaataaccg tcatacttgc tgtcggctac cagtgaactc acctttttga
     6721 ctgacacctt tgagtacttg ctgtcaacca ggagcgcagt cgccctttca acactgaaca
     6781 tccctacata gcgggcattg atctcagccc atccgagttc ataaacagtg gccttgccgt
     6841 atgccacaat cagggtattc agtggcttga cattcccgcg cgtgagcttg tcaactgtca
     6901 gatcaccgta cttgacattt gccgtgacaa gccctgaaag ctcatctata accgtgttgc
     6961 catacttgtt ggtgacatca aagttcaccc tggccggcat ctttattaca tagttgatgc
     7021 tgaaattgtt gtttgatccc ttccagctta tcgatgagaa gtcatcgctg aagatggtct
     7081 cagccttcag gttgccgcct tcctcggtga actccaccct gatcatgtca aggagcttcc
     7141 tcgccctgtc ggcagatgga tgttcaacct tcacggtaac atccacaaca acattattct
     7201 tgtcccacgt ctcggtgacc acagaaccga atttattgat tacggtcact gtcgagttgt
     7261 cacccggagt caactccctg tggaactcct ttttgacctc ttctgccgag gcggacaggg
     7321 ctactgagca cagcaacgct gcgaacagcg taacccgctg attagtaaac aacttcttca
     7381 tggctgactg gtttaacggt ttctgctttt atctctttga gctggtcaag tatataactc
     7441 atcacttcta ttttggtctg gtaatgtttg atcatggcat ctatgatccg ctggtcatcc
     7501 gggttggccc tgagttcctt ctgcagctca acgtagaccg aatccatgga agccatctca
     7561 accatcagtg cctctttctg ttcaggattg gacatgtcga tcagtgtgag ctcgttttcc
     7621 atcaggttca cctgctgcac ataatatttc tcaacctcgc ggtattcagg tgaaacctgg
     7681 ctgagtgtca tctttgtctt caggttgggc ctgacgaagt ggtcaaaggt ccacagtgag
     7741 gaaagtgtca ccagcagggt taccaccgca gccttaagca ggtatggaat gatacttctc
     7801 ttggctttgc cggcatgaag cctcgtagcc agcttgaagc tgaacctctc gaagtgcccg
     7861 tctgacggct ccctgtcgtc aaactcgctc ctgctcttcc tgattatgtc atctatcatg
     7921 ttcatcattc gtaaattttc ctgtttttaa gttcacccac cagcttctgc tttgcccttg
     7981 agagttgtga tctcgaggta ctgcttgaga tgttgagcat ctctgctatc tcatcgtggt
     8041 cataaccttc aatgagatag agagagagta tcacgcggta tccttccggc agccgctcaa
     8101 tggcagcctt tacctcctcc acccgcagtg ccagatcctc atggtaggca gtttcctcac
     8161 cgctgtcgtc gcggatccct gcgtgacttt caatgtcttc aaagatcgcc ttccttttcc
     8221 ccaggacatc aagtgatctg ttgaccacta tccgcttcag ccaagcgccg aagctcaccg
     8281 tccccgagta ggtgtttatc ttctcgaaag ctgagagaaa cgcttcctgc atcacatctt
     8341 cagcttccat ctcatcattt acaatacgca ggctggtgtt gtacatcgct ttatagtaaa
     8401 gcttgtaaac ctggaactgt gcccgctggt cacctgagcg acacccgtca atcaggtcct
     8461 ggtgtatgtt cctgaatgct gcttcaacat tttccatctt tttccaaaga tccggtttaa
     8521 gtatgtttga cgccgcaccc acgtatttga actcttcaat tcaatgacgg gggcgatgcc
     8581 cgaatgctgc atccaaggta gaaaaaagct gtaagaggat gaaaattttt ttatccctg
//
LOCUS       DAOPXU010000115         8251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_10359_length_8251_cov_15.040020, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000115 DAOPXU010000000
VERSION     DAOPXU010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10359_length_8251_cov_15.040020"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..400)
                     /locus_tag="PKK03_10275"
     CDS             complement(248..400)
                     /locus_tag="PKK03_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HNX84827.1"
                     /translation="MGLLRVLLCILFPPLAVVDKGCGSVLIIFLLTLCGWVPGVIGAL
                     IINNKK"
     gene            complement(462..3053)
                     /locus_tag="PKK03_10280"
     CDS             complement(462..3053)
                     /locus_tag="PKK03_10280"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNX84828.1"
                     /translation="MKSGFFFLRLLPTVVFPAAVLISCQYVDRAEYTAPGVSKLLATY
                     RYETIGDVRYGLTFRIDTGKNTPVEGIAVIELLRTAGREPLVLDFKVPGDHLKTVAVN
                     GKPTETGLVNGHIVIDRKLLSHRYNRVEISFRAGDLSLNRNDEYLYTLFVPDRASTAF
                     PCFDQPDIKGRFTLTVDIPATYRAMSNNPVISSDTTGGRVTLRFSETKPISTYLFAFA
                     AGNFDLVEREIDGVKMEMLHCETRPGYVENNVDEIFRLHHSALKWLEEYTQIPYPFDK
                     FGFVLLPPFQYNGMEHPGSIFYRASSLMLEASPTLNEQLSRASLIAHETSHIWFGDLV
                     TMKWFDDVWLKEVFAGYMSDKITGPDFPDMNHDLRFLLSRYPAAYEVDRTRGTNPIIQ
                     ELGNMKDAGSLYGNIIYFKAPIVMRQLEQLAGEENLKKGLQVYLKAYSWGNARWDDLV
                     GILEETSQKPLGEWSRMWIREAGMPVISPVITEEDGYRISLSEDDPAGTIRHWPQTMK
                     TMVITASDTVIAELEPAERDSWVTTAEKPLCIIPDISGRAYGTFLHDSTSTDYLTKHL
                     PGLKDPLLKGILWINLYENVVNGTISPDDFCETALRDLQVITDLQLRNYLSGRFSSVW
                     WNWLTPSERAARAADAEAVLRGKMESAGSPAEKRSWFGTLRNVTITSGGLDYLASLWE
                     KGTLPGPVILSEDELSTLALTLALKGHPGATRIIAEQRERIAGKERLERFDFVIPAVS
                     DNATVRDTFFESLRDPVNREREPWVLEALDYLHHPAVAERSEKYILPSLELLEEIKST
                     GDIFFPEGWISGTIAGHRSPGARATVEKFLDDHPDYPADLKLKILQAADRLFRQSCQI
                     VADDQEL"
     gene            3233..6340
                     /locus_tag="PKK03_10285"
     CDS             3233..6340
                     /locus_tag="PKK03_10285"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX84829.1"
                     /translation="MKKLLFILMISALTGSVSGQGQSAFLSDPAIAPDGSKIVFVYES
                     DLWMADRSGGTAHRITAMTGEESVPRFSPDGKWLAFASTQNGNADVYIMPVDGGALQQ
                     LTWHDGNDYPDSWSWDSKTVYFTSDRFNTFGAYTVPATGGTPARLFSDNYFDQAHHVV
                     EIPGGNGQEWYFTSSWESYMFAHRKRYRGENDPDIEYYNKGTGDYKRLTEWEGKDLWP
                     SVDRYGKLWFASDEGNAEYNLYTFDNGTKRQMTTFPTSIRRPQVSADGRYVVFARDYR
                     IWTFDVATGQSSPCEISVWSNENLATEIGYNSAGKITDFDVSVDGKKIAFVSRGRLFV
                     SDITGKFIKEMPTDRGERVQEVRWMKDNESLIYTRTVKGWANLFTISASAPEAEKQVT
                     RYERTLQNLLVSPDGEKALVNSGDSYIDLIDLKSFAVKSIIKDEFWFSPTQPRFSPDS
                     RYILYAAVRNFEQDVLVYDTREEKTYTLTNNGVDETDPYWSPDGRYIYLSADRYNAGF
                     PRGAGVSKLYRIPLYRFSESLRTEEYGKLFAKKASKDSLKVEIKIETEDISDRWEQID
                     VKGNDQSSPHVFTVRGKTMVLFNNSPNPRDRVMTKVELSPFDPPKSAAIGERSFSRLI
                     MAGDKFYALISGDVHEVKPAEGKADKIALSATFSKNLHDEFVQMFYENWATLAEHFYD
                     LNYHGADWKAMLSRYEQYLPLVRNRDNLRTIQNDMLGELNSSHLGFSSYGEEARPFYR
                     LQTNATGIIFNNDDPYVVDGWVKRSPTDLTDTPLRPGDRLVAVDGTPVVVSADRDRFF
                     TFPRLEKELTLRFERKGKEFDMTVAPVSSDAMKTLLYDSWVDENRRIVDSLSGGRVAY
                     VYMKDMGTQSLEKFLIDMTGIALGKEALILDIRNNRGGNVHDDVIRFLSQKPYLEWQA
                     RNGKRSPQPNFAPSGGPIILLVNEQSLSDAEMTAAAFRQLKMGTIVGTETYRWIIFTS
                     GRTLVDGSSTRLPAWGCYDLDGNDLEKTGVKPDVYVRTTFGDMQKGADPQLVAAVEEA
                     LGALH"
     gene            complement(6402..6770)
                     /locus_tag="PKK03_10290"
     CDS             complement(6402..6770)
                     /locus_tag="PKK03_10290"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNX84830.1"
                     /translation="MGSYVNGHLIKDESVAYETKYHWVIFFSLKSIFTLTLYAWFKRW
                     LSEFVITNRRIVIKEGFIARRTFEMNLSKIETVNVDQTVMGRILNYGSITIIGTGGTT
                     ETFHNIARPMTFRQKFQELC"
     gene            complement(6801..7187)
                     /locus_tag="PKK03_10295"
     CDS             complement(6801..7187)
                     /locus_tag="PKK03_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HNX84831.1"
                     /translation="MPRVFKALEIEKEEKEIMRDYLDRHMPHVICPDNVRRGEKFKVK
                     VRMGEEYPHPDEDDHYIGLIQLWNRETLMAEARYSAGVNGHQPSHVEVDFYIVAPDVS
                     MNLSAMAVCTKHGLWQSEDKAVKVTD"
     gene            complement(7270..8223)
                     /locus_tag="PKK03_10300"
     CDS             complement(7270..8223)
                     /locus_tag="PKK03_10300"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNX84832.1"
                     /translation="MEYKKLGARGPVVSTVGFGAWGISGRDWGTTDDEKSRKAIETAL
                     DSGVTFIDTADVYGFGHSEELIREVLDRRGDREQIVIASKAGNNFYPFLGQEHRTTPM
                     NIDYSVKHLVFAAEHSLRRLNLEALDILQLHSPDLEILERDEPWIALEKLKKEGKIKH
                     AGWSVQSFKETEQAHILERHHELLDVIQVRYNLLEREAEKVLFPMALRYGTGVIVRIP
                     LLFGLLSGKFKTDSRFGDNDHRRFNLSPEKLETYLSDLKRYQPLFDEYADYSKAQISL
                     RFCIAHPACHTVIPGGKNPGQVKENVVASDLGFIPHEKFSF"
BASE COUNT         1932 a         2114 c         2211 g         1994 t
ORIGIN      
        1 ttggggataa tctcggtttc tatgtcggtc acgaaaccgt acttgtattc tcccgatgtt
       61 acctcgccca gcagcttatc ctggtctttt tccatatgtt tctccttcaa cggtacattc
      121 actcaacaag tggcgccgtt taaagtttgt gctcgttttt agaatcgtgc aaaattaatc
      181 aaaaatggcg agatgaagga ggaatgtaaa tgtatgatga acagaggagc cggtaatctc
      241 tgagggatca tttcttattg ttaatgatca gggcgccgat aactccgggg acccagccgc
      301 agagggtgag gaggaagatg atcagtactg agccgcagcc cttgtctacc accgccaggg
      361 gagggaagag aatgcacagc agaacccgga ggagacccat aggaattgcg attttcgatt
      421 tgcgaatttc gaatacgaag ataggaaatt tcaatatcct tttacaattc ctgatcgtct
      481 gcgacgatct gacagctctg cctgaaaagc ctatccgccg cctgcaggat cttcagcttc
      541 aggtctgccg ggtaatccgg gtgatcatcg agaaacttct ccacggttgc ccgggccccg
      601 ggcgaccgat ggccggcaat ggtgcccgag atccagcctt ccgggaagaa tatgtcgccg
      661 gtgctcttta tctcctccag caactccagg gaggggagga tatatttttc tgacctctct
      721 gccacagccg ggtggtgcag ataatccagc gcttcgagca cccatggctc gcgctcgcgg
      781 ttgaccgggt cacggaggct ctcaaaaaag gtatcgcgaa ccgtagcatt gtcagatact
      841 gccggtatga caaaatcaaa ccgctccagt ctctctttcc ctgcaatccg ttcccgctgt
      901 tccgcaatga tccgggtggc gccggggtga cccttcaacg ccagtgtcag cgcgagggta
      961 gatagctcat cctcactcag aatgacaggg ccgggaaggg ttcctttttc ccaaaggctg
     1021 gccaggtaat ccaaacctcc tgacgtgatt gtcacatttc tcagcgtacc gaaccatgaa
     1081 cgtttttcag ccggacttcc agctgactcc attttgcctc tcagcaccgc ttcggcatcc
     1141 gcagccctgg ctgcccgttc ggaaggggtg agccagttcc accataccga tgagaacctg
     1201 cccgaaaggt agttacgcag ttgcagatcg gtgatgacct gtaggtcgcg gagtgccgtt
     1261 tcacagaaat catcgggact aattgttccg ttgaccacgt tttcatacag gttgatccag
     1321 aggatccctt tcagcagggg atccttcagt ccgggaaggt gcttcgtcag gtagtcggta
     1381 ctggtgctgt cgtgcaggaa tgtgccgtat gctcttccgg atatatccgg aatgatgcac
     1441 agaggctttt cagcagttgt gacccaggaa tcgcgttctg ccggttcaag ctcagcgatt
     1501 acggtatcgg atgcggtgat caccattgtt ttcatcgtct gcggccagtg cctgattgtg
     1561 cctgccggat catcctcact gagcgagatt ctgtatccgt cttcttccgt gatgaccggc
     1621 gaaatgaccg gcattcctgc ctccctgatc cacatcctgc tccattcccc gaggggcttt
     1681 tgcgatgttt cctccagtat tccaacgagg tcatcccagc gtgcgttgcc ccatgagtac
     1741 gccttcaggt aaacctgcaa tcccttctta aggttctcct ctccggccag ctgttcgagc
     1801 tgtcgcatca ctatcggcgc cttgaaatag attatgttcc cgtagagcga gccggcatcc
     1861 ttcatgttcc ccagttcctg tattatgggg ttggtgcccc gtgtacgatc cacttcatag
     1921 gcagccggat agcgggatag tagaaaccgc agatcgtggt tcatgtcagg gaaatcaggc
     1981 ccggtaatct tatctgacat gtacccggca aagacctctt taagccagac gtcgtcgaac
     2041 cacttcatcg taaccaggtc accgaaccag atatgcgatg tctcatgtgc aatcagcgag
     2101 gcccgcgaaa gctgttcgtt aagggtgggg gaggcttcga gcatcagaga ggaggctctg
     2161 tagaatatcg atcccgggtg ttccataccg ttgtactgga atggtggcag aagcacaaaa
     2221 ccaaatttgt caaagggata aggaatctga gtgtactcct caagccactt cagcgcactg
     2281 tgatgaagcc ggaagatctc atccacgttg ttctcaacat aacctgggcg ggtctcgcag
     2341 tgcagcatct ccatcttcac accgtcaatt tccctttcca caaggtcaaa attgcctgcg
     2401 gcgaaggcaa acaggtatgt gctgatcggc tttgtctcgc tgaagcgaag ggtgaccctt
     2461 ccgcccgtag tgtcagagga gatgacggga ttgtttgaca tcgcccggta agttgccggg
     2521 atatccaccg tcagtgtgaa ccgccctttg atgtcaggct gatcaaagca cgggaaggct
     2581 gttgaggccc tgtccgggac gaacagggta taaaggtatt catcgtttct gttaagggag
     2641 agatcccctg cacggaagct tatctccaca cggttgtaac ggtgcgataa cagtttcctg
     2701 tcaatgacta tatggccgtt aacgagtcca gtctcagtcg gtttcccgtt tacggctact
     2761 gttttgaggt gatccccggg caccttgaaa tccaatacca gaggttcacg gccagccgtt
     2821 cgtaagagct caatcaccgc aattccttcc accggtgtat tcttcccggt atcgattctg
     2881 aaagtcaggc cgtaccttac atcgccgata gtctcatacc tgtatgttgc caacagtttt
     2941 gacacacccg gggcagtgta ttccgccctg tcgacatatt ggcaggaaat aagtacggca
     3001 gcgggaaaaa ctacagtcgg cagcaggcgc aggaagaaaa atccggattt catggtggct
     3061 ataaaggttt ctcaaatata gtcaatcatg ctgaagagcc cgtcttaagc catgtttaaa
     3121 aggcatgctg ccgtaatttc acacctgtca ggccaacccg gtgctgcaaa acatcacaac
     3181 aggcaaaacg atattcccta tctttggaat agtacgtgct aacccaaata ccatgaaaaa
     3241 actgttattc attctgatga tatccgcact taccggcagt gtcagcggcc agggccagtc
     3301 tgcctttctg tctgatccgg caatagcacc tgacggatca aaaattgtat tcgtgtatga
     3361 gagcgacctc tggatggctg accgcagcgg tggtactgca caccggatca cagcaatgac
     3421 aggagaggaa tcagttcccc gcttttcacc tgacgggaaa tggcttgctt ttgcatcaac
     3481 acagaacggt aacgccgatg tatacatcat gccggtggat ggtggcgctc tgcagcagct
     3541 gacatggcat gacggcaatg actatcctga cagctggtca tgggattcga agacagtcta
     3601 ttttacctcc gacaggttca atacatttgg cgcctacacc gttcccgcaa cgggaggcac
     3661 acctgcgcgc ctcttctcgg ataactactt tgatcaggct catcatgtgg ttgaaatccc
     3721 gggaggcaac ggacaggagt ggtattttac atcttcgtgg gaaagttaca tgtttgccca
     3781 caggaaaaga tacaggggag agaatgaccc tgacatcgag tactacaaca agggtacagg
     3841 tgactataaa cggctgaccg agtgggaagg caaggacctg tggccttccg ttgaccgcta
     3901 cgggaaactc tggttcgcct ccgacgaggg caatgccgaa tacaaccttt acacttttga
     3961 caatggcaca aagcgacaaa tgacaacctt cccgacctcc atccgtcgtc cgcaggtgag
     4021 cgcagacggc aggtacgtgg tttttgcgcg tgactacagg atatggacct tcgatgtcgc
     4081 caccggccag agttcgccct gcgagatcag cgtctggtca aatgagaacc tggctacgga
     4141 gataggatac aacagcgccg gaaagatcac cgactttgat gtgtcagttg acggcaaaaa
     4201 gattgccttc gtctcacgcg ggaggctctt tgtctcggat attaccggga aatttattaa
     4261 ggagatgccc accgacaggg gcgagagggt acaggaggtc agatggatga aggataatga
     4321 gtcgctcatc tatacccgca cagtgaaagg atgggctaac ctgttcacaa tctccgcatc
     4381 ggcgcctgag gctgagaagc aggtcacacg atatgagcgc accctgcaga acctgcttgt
     4441 cagccctgac ggtgagaaag ccctggtcaa cagcggcgac agctacatcg atctgattga
     4501 ccttaagagc tttgcagtga aaagcataat caaagacgag ttctggttca gcccaaccca
     4561 gccccgcttc tcacctgaca gccggtacat tctttatgcc gcagtccgga acttcgagca
     4621 ggatgttctt gtctacgaca ccagggagga gaaaacgtat actttaacca ataacggtgt
     4681 tgatgagacg gatccttact ggtcacctga cggcaggtac atctacctga gcgctgaccg
     4741 ttacaatgca ggcttcccgc gaggggcagg cgtcagcaaa ctctatcgca tcccgcttta
     4801 cagattcagc gagtcgctgc gcacggagga gtatggaaag ctcttcgcga agaaagcttc
     4861 aaaggacagt ctgaaggttg aaattaaaat tgagactgag gatatttctg accgctggga
     4921 acagatcgat gtaaagggca atgaccagtc atcgcctcat gtattcacgg tccggggtaa
     4981 gacgatggtg ctcttcaaca actctcccaa tccgcgcgac agggtgatga cgaaggtgga
     5041 gctctctccg tttgatccgc cgaagagcgc agccattggc gagaggagtt tcagcaggct
     5101 gataatggcc ggtgacaagt tttacgcact tatctccggt gatgtccatg aggttaaacc
     5161 ggccgaaggt aaggctgaca agatagctct gtctgccacc ttcagcaaga acctgcacga
     5221 tgagttcgtt cagatgtttt acgagaactg ggctaccctg gcggaacact tctatgacct
     5281 gaattatcac ggcgcagact ggaaggcgat gctctcgagg tacgagcagt acctgccgct
     5341 ggtacgtaac cgggacaacc tgaggaccat tcagaatgac atgctcggcg agcttaactc
     5401 atcgcatctc ggattcagca gctacggcga ggaggcgaga cccttctaca gacttcagac
     5461 caacgctacg gggattatct tcaataatga tgatccgtat gttgtggatg gatgggtgaa
     5521 gcgctcaccg actgacctga ccgacacgcc gctccggccc ggcgacaggt tggtggcagt
     5581 tgacggaaca ccggtggttg tgtctgcaga tcgtgacagg tttttcactt tcccgcggct
     5641 tgagaaggag ctgacactcc gctttgagcg gaaggggaag gagtttgaca tgacggtggc
     5701 gcctgtcagt tcagatgcga tgaagacgct gctttatgac agctgggtgg atgagaaccg
     5761 aaggattgtc gacagtttgt ctggtggcag ggtggcttat gtctatatga aagacatggg
     5821 gacgcagagc ctcgagaagt tcctgattga catgacgggg atagccctcg gcaaggaggc
     5881 gttgatcctc gacatccgca acaacagggg aggcaacgtc catgatgatg taatcaggtt
     5941 cctctcgcag aagccctacc tagagtggca ggcgcgcaac gggaagcggt cgccgcagcc
     6001 caacttcgca ccgtcaggcg gaccaatcat tcttttggtt aatgagcagt cgctgagcga
     6061 tgccgagatg accgctgctg ctttccggca gcttaagatg gggacgatag tgggtaccga
     6121 gacatacagg tggataatat tcacctcggg gcggacactg gttgacggat cctcaacacg
     6181 cctgccggcg tggggttgtt atgacctgga tgggaatgac ctggagaaga ccggggtcaa
     6241 gcctgatgtt tatgtacgca ccacattcgg tgacatgcag aaaggggctg atccgcagct
     6301 ggttgccgct gtagaggaag cattgggagc actgcattaa gatgttggcg gaattcgcaa
     6361 ctcacaaatc gcaactccca aatcgcaaat cctcaggtcc ttcagcacag ttcctggaac
     6421 ttctgcctga atgtcatcgg gcgggctatg ttgtggaacg tttccgtggt gccgccggta
     6481 ccgatgatgg ttatggagcc gtagttgaga atgcgtccca tgaccgtctg gtccacgttg
     6541 accgtctcta tcttcgagag attcatctcg aaggtacgcc gtgcaataaa accctccttg
     6601 atgacaatcc ggcggtttgt gatgacaaac tcagatagcc atctcttgaa ccaggcatag
     6661 agagttagcg tgaatatcga tttcagtgaa aagaaaatta cccagtgata tttggtttca
     6721 taggctactg actcatcctt gatgaggtgc ccgttgacgt agcttcccat tattcggttt
     6781 gattatttgt cgtaaaatta tcagtcggtt accttgacag ccttgtcttc gctctgccag
     6841 agcccgtgct tggtgcatac tgccatggcg ctgaggttca ttgaaacgtc aggggcgacg
     6901 atatagaaat ccacctcaac atggctgggc tgatggccgt taactccagc cgaataacgt
     6961 gcctcagcca tcagtgtctc gcgattccag agctggatca ggccaatgta atgatcatcc
     7021 tcatcggggt gcggatattc ctcgcccatg cggaccttta ccttgaactt ctcgccccgc
     7081 ctgacgttgt cggggcagat gacatgcggc atgtgacggt cgagataatc acgcattatc
     7141 tccttctctt ccttttctat ctcaagtgct ttgaatactc ttggcatggc tttttgtttt
     7201 tagtaacaat taatgcaact gaattatcag tcccggtgat gtaaatttag aaagaattcc
     7261 ggtcagtttt caaaaactga atttttcatg cgggataaaa cccaggtctg atgcaacgac
     7321 gttctccttt acctgtccag ggtttttccc gcccgggatt actgtatggc aggcggggtg
     7381 tgcaatgcag aacctgaggc tgatctgtgc ctttgaataa tcagcgtact catcgaacag
     7441 cggctggtac cttttaagat cggaaagata agtctcaagc ttctccggtg agagattgaa
     7501 ccggcggtga tcattgtcgc caaaccggga atcagtttta aacttgccgg acagcaggcc
     7561 gaacaggagt ggaatccgca ctatcacacc cgttccgtag cggagggcca tcgggaagag
     7621 gaccttttcg gcctcgcgtt ccagcaggtt gtacctgacc tgtatgacat caagaagctc
     7681 atggtggcgc tcaaggatgt gtgcctgctc agtctccttg aaggactgca cgctccagcc
     7741 ggcatgcttg atttttcctt ccttcttcag cttctccagg gcaatccatg gctcatcacg
     7801 ctcaagtatc tcgaggtcag ggctgtgcag ctgaagtatg tcgagcgcct ccaggttgag
     7861 gcgccggaga ctatgttccg cggcaaagac gaggtgttta acagagtaat caatgttcat
     7921 cggagttgtt ctgtgctcct gtcccaggaa ggggtagaag ttgtttcctg ccttggaggc
     7981 gatgactatt tgttccctgt cgccacgacg gtcaagtact tcgcggatca gctcctcgga
     8041 gtggccgaag ccgtaaacgt cagcggtgtc aatgaatgtc accccggagt cgagagctgt
     8101 ctctatcgct tttcgtgact tttcatcatc tgttgtgccc cagtcgcgtc cgctgatacc
     8161 ccatgcgccg aagccgacgg tggagacgac gggacctctt gcaccaagtt ttttgtattc
     8221 catgtatttt gttttttgcc ggtattaatt a
//
LOCUS       DAOPXU010000116         8239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_10382_length_8239_cov_11.554252, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000116 DAOPXU010000000
VERSION     DAOPXU010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8239
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10382_length_8239_cov_11.554252"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..3052
                     /locus_tag="PKK03_10305"
     CDS             224..3052
                     /locus_tag="PKK03_10305"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNX84833.1"
                     /translation="MKKGLKITGIILVSLIVLILAAGLIIPTVFRDRIKEKVQTAINN
                     SVNANVSFDGYRLSLFKAFPNASFSLENLSVTGKGDFEGDTLAALKTAGIVINLKSLF
                     GDRGYEVKSVVIDEPFVNAIVNKDGKANWDIAKVSGEPGNGTGDQAGTAAAAGKGPEG
                     EEPSGLKLMLRKFAINNGRVNYTDYESDMQAAVSDLDFLLSGNMSGSQTGLDLEMNAG
                     KVDFVMDEIPWLTGAALSFRAGIDALLDSMKFTLRDNVLKLNDIALNFSGTAAMPGDD
                     IDLDMVFSAPGTSFKSLLSLVPAFYMKGYEDLKAAGTVALDGAVKGVFSSADSTLPDV
                     LLNLKVSDGLISYPALPEKISEINIDGKVQTVGSDMDKTVAEVSRFHFVLAGNPFDIS
                     LTMATPLSDPAVNVMAKGRIDLAKLQQAVPLDSVTLNGLLDVSLDMAGRMSMIENREY
                     EKFRAAGNMNIADMAVAMAGMPDMKISKASFSFSPAFAELTGMNASMGEGSDFSLSGR
                     LENYIPYLFSDGILRGNLSLKSDHVDLNEIMEYIPSDTVETDTVPMEVIRIPEDIDFT
                     LDALVGTLNYGMLAANDIRGNIVIREGVVTVSETGMKALGGSLLVNAVYDTRDTLKPA
                     VDAEMLVSAVSIKDAFGAFKTVKRLMPAAEGLGGNVSAKIDFSSILGTGMMPLLNTLS
                     GTGEINSESVQVLQSTSFDRMKNVLKIDPAYTNILKDIRATFIINDGRLWIKPFDTKL
                     GRIKLNVSGDQGLDQTINYLVKAEIPREELGQAAGALMSTFASQAAALGFSAAPPEVI
                     RVNLNVGGTVGNPAFTPSFAGGSVSSAVTALTDTVKSEVTEKVNEAARQQADRIIKEA
                     EDNAKVIREKAASSAETIRSEADLRGKKLIKDAEASGPFAVAAAKKAAEALNREADKR
                     ATQIVAEANKRADEVLATARTKADEILR"
     gene            3233..6070
                     /locus_tag="PKK03_10310"
     CDS             3233..6070
                     /locus_tag="PKK03_10310"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNX84834.1"
                     /translation="MKKQLFNLLLIVTILLPLNSFSQTLGGTITGVVRDQSTGETMPG
                     VNVVIEGTLRGASTGPDGEFTITGVAPGTYSLSLTFISYKPVRVEGVEVRTGSVSVIN
                     VAMSDQSVELDDIVVVATKRTDSEVSVMNSIRTSPVVVSGISAQLISRTQDKDASEVV
                     RRLPGVSIVDDRFIVVRGLSSRYNNVWLNNATAPSLEADARSFSFDLVPSSMIENIYI
                     LKSPTAELPADYSGGFIKISTTGIPSENRFSVSYGTTFRQYATFGDYLAERGGKTDWL
                     TMGTGQRALPDAMPVHLDLYESATNTDIRNRVTDLGRSLSNSWDAVQATALPDQKLAL
                     GISRRFEKGSFIAGNVTSLTYSYSNTFREAVNNSYSIYNYAYDTPGILDEYIDRQYRT
                     SARVGLMHNQTLYPYQGLKLEFRNFFTSTAGSGVSVREGRDWYNNGRSVRSGEIKYSH
                     RLIYTGQLGAEQILNDGRSVIDFTAGYARSARTEPDIRRYRYISNDGENSPYIILFGN
                     NPDLSSVSRMWLDLDETNLSGMLNLTQKFSEGMIDWEIRTGIFAENRDRSFRARNFGY
                     AVSSDTSPFASTSLPVQEIFAAENINLTDGIRLKEITALSDSYEASAMVLAGYLSFRF
                     RFGTRTDLSAGVRIEKERQELSSYRQGTRIPVKVVRDTVNLFPSGNLAVNLSSSSLVR
                     LTYGMSVNRPEFREMAPFYFVDFDQNAGIYGNEGIKSAYIHNLDLRFELYPGNNETFN
                     AGIFYKHFTNPIEVSIRGNNPTQFSFDNIGSAYSFGLETEARKNLGFITDRLEKLTAV
                     VNLSLIRSSVAFDRPLQGQSPYIVNIGLFWQDTDRGLMASVVYNRIGKRIIAVGRPSP
                     NSWESIPDIYEMPRNEADLAVSKKISKMLEIKAGIKDLFGEQVVFQQHVNSIVDMTNY
                     GGDGIKAFDRNQNTRSWYPGRQYSFGLSLKF"
     gene            6100..7731
                     /locus_tag="PKK03_10315"
     CDS             6100..7731
                     /locus_tag="PKK03_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNX84835.1"
                     /translation="MKTNILSAIARVMALCIVLTAMVSCEKEIPVSFLSLDKDEATVQ
                     VGADLTLLPVIDPLNATNREVSWTSSNSSVATVADGVVTGVALGNATITAASSENPLL
                     TAECEITVVPSTGQVITVSGDITTDTRWYAQAKYHLSGFVYVRNNSTLTIDAGTIIKG
                     VSNTKATLIIERGSRIIAAGTASQPIVFTSDKPAGQRATGDWGGIVLCGKAKTNKHDD
                     GEGVGIAEGGIGSKYGGTDDNDNSGVLQFVRIEFPGIPLTSTANSEINGLTLYSVGKA
                     TVIDHIQVSYSGDDSFEWFGGNVNAKYLVALGGLDDGFDTDNGFSGKIQFGLILQDPL
                     KSDQSGSNAFESDNDADGSLLTPVTSPVFSNVTAIGPLAVTATLPAETKHEKALHLRR
                     GTMTSVYNSVFVGFPQGLSIDGQKGNAPTRADQNELQIENTILAGMTTLYVEKTGTVA
                     VPYTVAQHQSYFTATARNNTDNMTMAEITGAGLINLTSPVLIPQAGSPLLTGASFTNA
                     RLSDTFFTITTYRGAFGTENWTSGWCNWNPQNSVY"
     gene            complement(7858..8205)
                     /gene="rplS"
                     /locus_tag="PKK03_10320"
     CDS             complement(7858..8205)
                     /gene="rplS"
                     /locus_tag="PKK03_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNX84836.1"
                     /translation="MDLMKVVEKEFASGVEFPKFRAGDTVTVHYKIKEGNKERIQQYR
                     GVVIQRSGAGMTATFTVRKMSGNIGVERIFPVTSPFIDKVEVNKYGKVRRARIFYLRN
                     LTGKKARIKEKIF"
BASE COUNT         2192 a         1933 c         2094 g         2020 t
ORIGIN      
        1 aaccttctga tccgtagtca gatgctctat tcagttaagc tacgggacct tttgcgggtg
       61 caaatatatt aaattttcgt tatcacttca gaagaaaatt tatttatccg acctgattac
      121 ctacttttgt gaatgacatg catttatttg ctggtcacca ttggctgcgc cgaactaatt
      181 tcagcgcttt ttcgttaaac tcaggaaatt atcagatgca acaatgaaaa aaggactgaa
      241 gattaccgga attattcttg tttcccttat tgttctgata ctggccgccg gattgatcat
      301 cccgacagtc ttcagggaca ggataaaaga aaaggtgcag accgcaataa acaacagcgt
      361 gaatgcaaat gtatcatttg acggatacag actgagcctt ttcaaagcct ttccgaatgc
      421 atcattctca ctcgaaaacc tcagcgtgac gggaaagggt gattttgaag gtgataccct
      481 cgctgcgctt aaaaccgcag gtatcgttat taatcttaaa agccttttcg gcgacagggg
      541 atatgaggtc aagtcagtcg tcattgatga accctttgtc aatgccattg tgaataaaga
      601 cgggaaggcc aactgggata ttgcgaaggt gtctggggaa cccgggaatg gtaccggtga
      661 tcaggccggt actgccgccg ctgccgggaa aggccctgaa ggagaagagc cttccggctt
      721 gaagctgatg cttcgtaaat ttgcaattaa caacgggcgt gtgaattaca ctgactatga
      781 atcagacatg caggctgccg ttagtgatct cgatttcctg ctatcaggaa acatgagcgg
      841 ttcgcagacc gggcttgatc ttgaaatgaa tgccggaaag gttgatttcg tcatggatga
      901 aattccctgg ctgaccggcg ctgccctgag cttcagggcc ggaatagatg cactgcttga
      961 ctcaatgaaa tttactctaa gagacaacgt cttaaaactg aatgatatag cgctgaattt
     1021 ctccggtact gcagccatgc cgggtgatga tatagatcta gatatggtct tcagcgctcc
     1081 cggaacctca ttcaagtcac tcctctcact ggtgcctgct ttttacatga agggatatga
     1141 ggatctgaaa gccgccggca ctgtagctct tgacggcgct gtaaagggag ttttcagttc
     1201 agccgacagc accctgccgg atgtcttgct gaacctgaag gtcagtgacg gtttgataag
     1261 ttaccctgcg cttcctgaaa aaatcagtga gatcaatatt gacggaaagg tgcagactgt
     1321 aggcagtgac atggacaaaa ccgttgctga ggtgagccgt ttccattttg tacttgccgg
     1381 caatccattt gatataagtc tgacgatggc caccccgttg agtgacccgg cggtcaatgt
     1441 catggcaaag ggcaggattg accttgccaa gcttcagcag gctgttccac tggacagcgt
     1501 caccctgaat gggctcctcg acgtgtcact tgacatggcc ggccggatgt caatgattga
     1561 aaacagggag tatgagaagt tcagggcagc aggcaatatg aatatagcag acatggctgt
     1621 ggcgatggcc gggatgcctg acatgaaaat cagcaaagcg tcattttcct tcagccctgc
     1681 atttgctgag ctgacaggca tgaatgcatc aatgggggag ggaagcgact tctccctgtc
     1741 gggacgactt gaaaattata tcccttatct tttctctgat ggaatcctcc ggggtaacct
     1801 gtcgctgaaa tcagaccacg ttgacctgaa tgagattatg gaatatatac catctgacac
     1861 ggttgagact gataccgttc cgatggaggt gataaggatc cctgaagata tagactttac
     1921 ccttgacgct ctggttggca ccctgaacta cgggatgctg gcagcaaatg acataagagg
     1981 caacattgtt atccgtgagg gtgttgtgac agtaagtgag acagggatga aggcattggg
     2041 aggctcgttg ctggtgaatg ccgtctatga tacccgtgat accctgaaac ctgccgtaga
     2101 cgcggaaatg cttgtcagcg cggtcagcat aaaggatgca ttcggcgcct tcaaaaccgt
     2161 taaacggctg atgcccgcag ccgaaggtct gggaggcaat gtctcagcga agattgactt
     2221 cagcagcatc ctcggtaccg ggatgatgcc gctgctgaac accctctcgg gaaccgggga
     2281 gatcaattcc gagtcagtac aggtgttgca gtcgacaagt tttgaccgga tgaagaatgt
     2341 tctgaagatc gatcctgcct ataccaatat tctcaaggac atcagggcta cattcatcat
     2401 taatgacggg agactgtgga taaaaccttt tgacacgaag cttggcagga taaagctgaa
     2461 tgtgtcaggg gatcagggac tggatcagac cataaattac ctggtgaagg cggagattcc
     2521 ccgggaagag ctggggcagg cagcgggggc cctgatgagt acctttgcct cacaggcagc
     2581 tgccctgggc ttcagtgctg caccacctga agtgataagg gttaacctga atgtgggagg
     2641 cacagtagga aatccggcat tcactccatc tttcgccggc ggcagcgttt catctgccgt
     2701 gactgcactg actgacaccg tgaagagcga ggtaacggaa aaggtcaacg aggcagccag
     2761 acagcaggca gacaggatca taaaggaggc tgaggataat gcaaaggtga tcagggagaa
     2821 agctgcatca tcagctgaaa cgatacgcag tgaagccgat ctgaggggca aaaagctcat
     2881 caaagatgcg gaggccagtg gtccgtttgc cgtggccgcc gcaaaaaagg cagcagaagc
     2941 actgaacagg gaagctgata aaagagccac acagatcgtt gcggaagcca acaagagagc
     3001 tgacgaggta cttgcaacag ccaggaccaa agccgatgaa attctcaggt aatttgtaac
     3061 aggacaggct cagatattct gaagaggttg taataacctc tttttttttg attaccatca
     3121 aatcagcctg tcacaatcat gtaacataag tgtcatctat tcgtaaccgg ttttttacac
     3181 tcctgtaaca tcagcggctg acttttgcac tgtattcaag tcagccatca aaatgaaaaa
     3241 gcaattattt aaccttttat tgatcgttac aattctgctt cctctcaatt ccttttcaca
     3301 gactttgggc ggcaccataa ccggtgttgt acgtgaccag tcaaccggcg aaacaatgcc
     3361 cggtgtcaat gtggtaattg aaggaactct caggggcgcc tcaaccggac ccgacggaga
     3421 gtttaccatt accggcgtag ctcccggaac ctacagtctg agtttaacct tcatatcata
     3481 caaacccgtg agggtggagg gtgttgaggt cagaaccggt tctgtttcgg tcatcaacgt
     3541 tgccatgtct gaccagtctg tcgaactgga tgacattgtg gttgttgcca ctaaacgcac
     3601 agacagcgaa gtgtcggtaa tgaacagcat caggaccagc ccggtggtcg tttccggtat
     3661 ctctgcccag ctgatcagcc gcacgcagga taaagatgcc tcggaggttg taaggcgact
     3721 tccgggagta tccatcgtgg atgacaggtt catcgttgta aggggactct catcgaggta
     3781 caacaatgtc tggctcaata atgcaacggc tccgagtctc gaggctgatg caaggtcctt
     3841 ctcgtttgac ctggtcccgt cctcaatgat cgagaatatt tatattttga agtcgcccac
     3901 ggcagagctc cctgcagatt attccggcgg gttcattaag atatcaacaa ccggcattcc
     3961 gtcggaaaac aggttttcag tatcctatgg tactacattc agacagtacg caacattcgg
     4021 cgattacctc gcagagagag gtggtaagac agactggcta actatgggga ccggacagag
     4081 ggcactgcct gatgcaatgc cggttcatct tgacctttat gaaagcgcca ccaatacgga
     4141 tataagaaac agggttactg accttgggcg gagcctgagc aactcatggg acgcggttca
     4201 ggctacagcc ctgcccgacc agaaactggc actcggtatt tcaaggcggt ttgagaaggg
     4261 aagcttcatt gccggtaatg tcacatctct gacttacagc tattccaaca ccttcaggga
     4321 agctgtgaac aacagctact caatatataa ttatgcctac gacactccgg gtatactgga
     4381 tgaatacatt gacaggcagt acaggacaag tgctcgtgtg gggctgatgc ataaccagac
     4441 cctctatcca taccagggat taaaactgga gttcagaaac ttcttcacca gcactgccgg
     4501 gtcaggggta tcagtcagag agggacgcga ctggtacaac aacggtcggt ctgtcagatc
     4561 aggtgagata aaatactctc acaggctgat ctacacaggg cagcttggcg ccgagcagat
     4621 tttgaatgat gggaggtcag tgattgattt cactgcaggc tatgctcgtt cggccagaac
     4681 ggagcctgat atcaggcggt accgttatat cagcaatgat ggcgagaact caccatacat
     4741 cattctcttc ggaaacaatc ctgacctttc atctgtatcg aggatgtggc tggatctcga
     4801 tgaaacaaat ttgtcgggaa tgctcaacct tacacagaag ttcagtgaag ggatgatcga
     4861 ctgggagatc agaacaggga tttttgcaga gaaccgtgac aggagcttca gagcacggaa
     4921 tttcggatat gctgtatcat ctgacacctc gccatttgct tccacttctc tcccggtaca
     4981 ggaaattttt gcagctgaaa acatcaatct tacagacggt atcaggttaa aggagattac
     5041 cgccctttcc gattcatatg aagcctcggc gatggtgttg gccgggtacc ttagtttcag
     5101 gttcaggttt ggcacacgga ccgatttgtc agctggtgtg aggatcgaaa aggagaggca
     5161 ggaactgtca agctacagac agggcaccag aataccggtc aaagttgttc gtgacacagt
     5221 caacctcttt ccttcaggca atctcgccgt aaacctcagt tcatcgagcc tggtgaggct
     5281 gacctacggg atgtcagtca accggccgga attcagggag atggctccgt tctacttcgt
     5341 tgatttcgac cagaatgcag gaatctacgg caatgaggga atcaaatcag cctatattca
     5401 caatctggat cttcgttttg aactttaccc cggcaacaac gaaacattca atgcaggtat
     5461 tttttacaag cacttcacca atcccattga ggtctcaata aggggcaaca atcccactca
     5521 gttttcattt gataatatcg gctctgcata cagcttcggg ctggaaacgg aggctcgtaa
     5581 aaacctgggg ttcatcactg acaggcttga gaaacttact gccgtggtca atctttcgct
     5641 gatcaggagt tccgtggcct ttgaccggcc cctgcaggga cagtcacctt acatcgtcaa
     5701 catcggcctt ttctggcagg atactgacag ggggctgatg gccagtgtgg tatataaccg
     5761 tataggtaag aggattatag cggttggacg gccttcgccg aactcctggg aaagcatacc
     5821 tgatatttac gagatgccgc ggaatgaggc tgacctggct gtaagcaaaa aaattagcaa
     5881 aatgcttgag ataaaagcag ggatcaagga cctgttcggt gagcaggtgg tattccagca
     5941 acatgtcaat agtattgttg acatgactaa ttacggcgga gatggcataa aggcatttga
     6001 caggaaccag aacaccaggt cgtggtaccc gggcaggcag tattcgtttg gactatcact
     6061 taaattctaa ggaaacaatc atttaataaa acacgaatca tgaaaacaaa cattttatca
     6121 gcaattgcca gggtgatggc actttgcata gttctgacag cgatggtgtc atgcgaaaag
     6181 gaaataccgg tctctttcct cagtcttgat aaggatgagg ccacagtgca ggttggagca
     6241 gatctgacac tgctgcctgt cattgacccc ttgaatgcca caaacaggga ggttagctgg
     6301 accagcagca attccagtgt ggccacagtg gcagatgggg tagtgaccgg agtagctttg
     6361 ggtaatgcaa ccattaccgc agcttcgtca gaaaatcctc tgttaactgc agaatgcgaa
     6421 attacggttg taccatccac aggtcaggtc attaccgtct ccggagatat aaccactgac
     6481 accagatggt acgcacaggc aaaataccat ctatccggat tcgtctatgt caggaacaat
     6541 tcaactctta caatagatgc tggcacgatc atcaaggggg tatcaaacac caaggcaacc
     6601 ctgatcattg agagaggttc gaggataatt gctgcaggca cagccagcca gccgatagtt
     6661 tttacatccg ataaaccggc tggtcagagg gcaaccggag actggggcgg gattgtttta
     6721 tgcggaaagg cgaaaaccaa caagcatgat gacggtgaag gtgttggcat tgctgaagga
     6781 ggcatcggat ctaagtacgg tggtactgat gacaacgaca attcaggtgt gcttcagttt
     6841 gtcagaattg agttccctgg aattcccctc acttccacgg ctaacagcga gattaacgga
     6901 ctgacacttt actctgttgg gaaagcaact gttattgacc atattcaggt ttcatattca
     6961 ggtgacgact ccttcgaatg gtttgggggc aatgtaaacg caaaatacct cgtagctctg
     7021 ggaggacttg atgacggatt tgatactgat aacggattca gcggtaagat tcagtttggt
     7081 cttattctgc aggatccttt gaaatcggac cagtctggct caaatgcctt cgagagcgac
     7141 aatgatgcag acggctcgct tctaacacct gttacatctc ccgtattctc caatgtgact
     7201 gccatcggac ctcttgcagt aaccgccacc ctgcctgcag agactaaaca tgaaaaggca
     7261 ctgcatcttc gcaggggcac tatgacctcg gtatataact ccgtatttgt cggattcccg
     7321 caaggactct cgatagacgg tcagaaaggc aatgcgccga cacgtgctga ccagaatgag
     7381 ttgcagattg agaatacaat acttgcagga atgacaaccc tctacgttga gaagaccggc
     7441 accgtagctg taccctatac agttgctcag caccaatcgt actttacggc tacagccaga
     7501 aacaataccg ataatatgac catggcagaa atcactggcg caggtttaat aaaccttaca
     7561 tctccggttc tgatccccca ggctggatcc ccgctcctta ccggagcctc cttcaccaat
     7621 gcaaggcttt cagacacctt ctttaccata acaacctaca ggggggcctt cggaacggaa
     7681 aactggacat ccggatggtg caactggaac ccgcagaatt cggtgtacta gtccagaaat
     7741 tgatggtttg acaacctttt accgtggggg cacaccggag tggccccctt ttttcttttc
     7801 aggaacaaag aaaaagccgc ccggtgaggc ggctcttcca tgtttatgcc tgaagtatca
     7861 gaatattttt tccttgatgc gtgccttttt gccggtcagg ttcctgaggt agaagatgcg
     7921 cgccctgcgt accttgccgt acttgttaac ctcaaccttg tcaatgaatg gcgatgtaac
     7981 cgggaaaatc ctttctactc cgatgttccc tgacatcttg cggacggtga atgtagctgt
     8041 catccctgct cctgatctct ggataacaac accacgatac tgctgtatcc tctccttgtt
     8101 accttccttg atcttatagt gtaccgttac agtgtcgcct gccctgaatt tcggaaactc
     8161 gactcccgag gcaaactctt tttctactac tttcattaag tccatcgcgc aaatatgttt
     8221 atgtgatctt tactctttt
//
LOCUS       DAOPXU010000117         8042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_10741_length_8042_cov_15.556154, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000117 DAOPXU010000000
VERSION     DAOPXU010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8042
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10741_length_8042_cov_15.556154"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1677
                     /locus_tag="PKK03_10325"
     CDS             <1..1677
                     /locus_tag="PKK03_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNX84837.1"
                     /translation="PMFPLWTFGYWQSRERYRSQDELVGVVSKYRELGVPLDGIIQDW
                     QYWGNNYLWNAMEFLNADFYNPQKMVDDVHGMNAHMIISIWNSFGPETKQYRELEKIG
                     ALMDFKTWPMSGSDKWPPMLDYPSGVRPYDPFNPEARDIYWRYLNKGLFSFGIDGWWI
                     DSSEPDHMDFKPSDLDNKTYLGSFRKVRNAFPLMTVGGVYQHQRSVSSDKRVFILTRS
                     AFAGQQRYASNVWSGDVTASWTALRNQISSGLNFSLSNIPYWNSDIGGFFLSRYSKKL
                     EDAEYRELYARWMQFGAFCPMMRSHGTDAPREIYQFGKKGDRIYDAIEKSIKLRYSLL
                     PYIYSAAWDVTSNRSSMMRALAMDFPSDSKALDINDQYMFGKSFLVCPVTTAMYSRDT
                     KEDFSAVGKRELYLPSGTDWIDFWTGEKLRGGQIVIKEAPVDIIPLYVRAGAIIPIGP
                     DVQYATEKRWDNLEIRIYAGADGLFTLYEDENDNYNYEKGAYSNITFRWNDAGKTLTI
                     GDRKGSFPGMLSEQKFRIILVGKNKGTGTETPKQWDKEITYEGKRTVTKL"
     gene            1700..3274
                     /locus_tag="PKK03_10330"
     CDS             1700..3274
                     /locus_tag="PKK03_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HNX84838.1"
                     /translation="MKKYYFSLLILINSLLCLTVTGQADQARNPVIWADVPDMSVIRV
                     GNIWYMTSTTMHMSPGVPVMKSEDLVNWEMVTYAYSTLGDNDELNLIHGKNAYGRGSW
                     ASCIRYHNETFYVSTFSSTTNKTYIWSTKDIEKGPWKEVSFSPSFHDHTLVFDDDGRV
                     YLIYGNKKLYLVELNADLSGVKPGGINQVIIENSSAPSGPESGLGEGSQLFRKDGRYY
                     LFNITWPRGGMRTVVVHRADRLTGPWEGKLVFQDLGVAQGGMTDTPDGRWFAYLFRDY
                     GSVGRIPYIIPMTWENGWPVIGTDGKVPEMLDLPPGKGLIPGIVAPDEFKRKKGDPPL
                     PLVWQWNHNPDNSRWSVTQRKGFLRLTAGRIDSVFTQARNTLTQRTIGPECSGSTLID
                     VSGMKEGDFAGLALLQKKYGIVGVKYSNGIRSVVMVNAQSGSPEEIESIPLTRKKVYL
                     KAECDFTDRRDLAYFYYSLDGKTWKSIGNQLKMEYSMPHFMGYRYGLFNYATREPGGF
                     VDFDWFRITNERTESK"
     gene            3296..5908
                     /locus_tag="PKK03_10335"
     CDS             3296..5908
                     /locus_tag="PKK03_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HNX84839.1"
                     /translation="MTKTNLNHSRYPWKRSIFMVPFLGFLFGSVILNGQLLRLNEKGY
                     FEARGVNILVYSNQYTGFFFDEKTAGIEIIQQGVRTSTGGAVRLQNTPEQWDLIPAVT
                     GRKVDEANKTITTELNYKDFDFNSRISVTARDNGVEINVYLDRPLPRELEGNAGFNLE
                     FLPSAYFEKTYLVDGKPGNFPRYPSGNTRIESSDKKIPQYAGHTTFDDRGLGEFIVPL
                     PLATGKTLTLAPDDPARMVIIKGTETDLMLYDGRNLAQNGWFVVRSLLPANKTGKVLT
                     WYLEVNSVPDWTREPVIGFSQAGYVPAGEKVAVIELDMNDTPMNSAQVFQVTADGRSV
                     EKFIGPVKIWGKYLRYNYARFDFSSVREPGVYYISYGNHKTNPFVISPDVFENVWHPT
                     LDVWFPVQMDHMTVNEAYRIWHGASYLDDCLQAPLNHQHFDGYRMGDTTDTRFRPLER
                     IPNMAVGGWFDAGDFDIQTGSHNSVITRFVEAWEEFRPDRDQTFIDQETRYVDIHRPD
                     GKPDILQQIEHGTLNLVAQCENIGHPVTGIIVPAMHQYHHLGDAMTETDNLPYNPALK
                     PYETDGRSSGTPDDRWAFTTRQPYLDYSTAATLAAASRALRGFNDDLAERSLANAKRL
                     VEEADELGKNKTGNDPMMIMWSMMGIEAQLEAALQLYITTGEKKYAERFLGKIWSSLE
                     SQRGGREMAFQGAFSGMELLPALKALPYMDDSYKTKLKGYVVKYRKSLDEIEKQNPYA
                     VPITLGSWGGTSSVVAWGIANYYAFKAFPDVMDKEYVLRGMNYVLGCHPYSNISFVEA
                     VGTRSKKVAYGNNRADFSIIAGGIVPGLILLRPDYLENKDDWPFFWGQNEVTMAGSAD
                     YLFLAKAAAEIANN"
     gene            5953..7296
                     /locus_tag="PKK03_10340"
     CDS             5953..7296
                     /locus_tag="PKK03_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNX84840.1"
                     /translation="MRYLKLSIAITAMVAGTLAYSQNPVIRDQFTADPTARVFNGKMY
                     LFPSHDIPAPESFPRKDWFCMADYHVFSSSNLTDWTDHGVIVSQDKVDWVNPTSFSMW
                     APDCIERNGKYYFYFPANVKTGRGFGIGVAIADNPEGPYIPQPEPIAKARGIDPSVFI
                     DKDGQAYIYYSMGRIFVAKLKENMTELDSDPVVVGDLPTKGLVEGPFFFERNGIYYMT
                     YPHVENTVERLEYAMGDNPLGPFKVTGVIMDETPMGTWTNHHSLVQYNDQWYLFYHNS
                     AYSPKFDKNRSVCIDSLFFNEDGTIRKVIPTNRGVGITSAQTTIEIDRFSQVSPAGVT
                     VALIDTINPFRGWTAQLSGPGSWISYNAVDFGNKKLKSVRVNASSVTGGIIQIRLDNP
                     AGLLLAEIEIPGNTALSNVDAKLKKYSKGTHNLFVVLKDGNPVNIDWVQFRKKSK"
     gene            7336..>8042
                     /locus_tag="PKK03_10345"
     CDS             7336..>8042
                     /locus_tag="PKK03_10345"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HNX84841.1"
                     /translation="MKSLITGSLAALLMFSACSTSGKVSPATKEMVLWYDKPAGKAWL
                     DGLFIGNGYMGANVFGKVQNERIALNESTFWSGRPHDYNDPDAYKYFGQIRDLMFAGK
                     FREAEKLADEHFYGKPGAQQAYQPVGDLLLDFKVGSDSVKDYYRELDMETGIVRISYT
                     DGDVKMTREVFMSYPDHVMVMRISADKPGKVSVEAKLSSPFAEKTKVTDNRLTLSGTW
                     KYLPEEISWLVAKVEGPG"
BASE COUNT         2271 a         1857 c         2038 g         1876 t
ORIGIN      
        1 ccgatgttcc cgttgtggac attcggctac tggcaaagca gggaacgtta caggagccag
       61 gatgaacttg tcggcgtggt cagtaaatac cgtgaactgg gggtgccgct tgatggtatc
      121 atacaggact ggcagtactg gggtaacaat tacctctgga atgccatgga gttcctcaac
      181 gctgacttct ataatccgca aaagatggtt gatgatgtgc acggcatgaa cgcacacatg
      241 atcatttcca tatggaattc gtttggcccg gagacaaaac aatacaggga actggaaaag
      301 ataggggcgc tcatggattt caagacctgg ccaatgtccg gttccgacaa gtggcccccg
      361 atgctcgatt acccctcagg tgtaagacca tatgaccctt tcaatcctga agcccgcgac
      421 atctactgga gatacctgaa caagggcctg ttctcctttg gcattgacgg ctggtggatc
      481 gattcatcgg agcctgacca tatggacttc aaaccttctg atctcgacaa caagacgtac
      541 ctgggttcat tccggaaagt ccgcaacgct ttcccactga tgacagtagg tggcgtttat
      601 cagcaccagc gctcggtcag ttcagacaaa cgcgtgttta ttcttacccg ctcggccttt
      661 gcaggtcagc agcgttatgc ttccaatgta tggtcaggtg atgtcactgc atcatggact
      721 gcattgagaa accagatatc gtcaggactg aacttttcgc tgagcaacat accttactgg
      781 aacagtgata tcggcgggtt ctttctgagc aggtacagca aaaaacttga agatgctgag
      841 taccgtgagc tgtatgcccg ctggatgcag ttcggcgctt tctgcccgat gatgcgttcg
      901 cacgggaccg atgccccgcg cgagatttac cagttcggga aaaaaggtga caggatctac
      961 gatgccattg aaaaatcaat aaaactgcga tacagcctgc tcccttatat ctattccgca
     1021 gcatgggacg ttacatcaaa ccggtcgtcc atgatgaggg cactggcaat ggactttccg
     1081 tccgatagca aagcactgga tatcaatgac cagtacatgt tcggaaaatc attcctggta
     1141 tgtcctgtta caacggccat gtattcaaga gacacaaaag aggatttcag cgccgtcggc
     1201 aaaagagaac tttacctgcc ttcgggaaca gactggattg atttctggac aggcgaaaaa
     1261 cttcgtgggg gacagatagt gataaaggaa gcgcctgttg acattattcc gttatacgtc
     1321 agggccgggg caataattcc catcggtccc gatgtccaat atgccacgga aaagagatgg
     1381 gacaatcttg aaattcgcat ctatgcgggc gctgacggct tgtttaccct ttatgaagac
     1441 gaaaacgaca attataatta cgagaaagga gcttattcaa acattacctt ccgctggaac
     1501 gatgcaggga agacactgac aatcggtgac cgcaagggtt cttttcccgg aatgctctca
     1561 gagcagaaat tcagaatcat actggttgga aaaaataagg gcacaggaac tgaaacaccc
     1621 aaacagtggg ataaagagat aacctatgaa ggtaaaagga cagtgactaa actataaata
     1681 ttgagatcaa agcagcgtca tgaagaaata ctatttcagc ctcctgatac taatcaattc
     1741 ccttctttgc ctgacagtca ccggtcaggc ggaccaggcc cgaaatccgg tgatctgggc
     1801 tgatgttccc gacatgtcag taatacgtgt cgggaacatc tggtatatga ccagcaccac
     1861 catgcacatg tctccgggcg ttccggttat gaagtctgag gatcttgtca actgggaaat
     1921 ggtcacttat gcttacagta cgcttggcga taatgacgag ctcaacctca ttcacgggaa
     1981 gaatgcctac gggagaggct cctgggccag ctgtatcagg tatcataatg agaccttcta
     2041 tgtatcaaca ttctcctcca caaccaataa aacatatatc tggtcaacaa aagatataga
     2101 aaaggggcca tggaaagagg tctcattcag ccccagtttc catgatcata ccctggtgtt
     2161 tgatgatgac ggtcgtgttt atctgattta cggtaataag aaactatatc tggttgaact
     2221 caatgcagac ctgtcaggag taaaacccgg aggaataaac caggttataa ttgaaaactc
     2281 aagtgcaccg tcgggtccgg aaagcgggct gggagaaggt tcgcagttgt tcaggaagga
     2341 tggcagatac tacctgttca atataacttg gccgcgcggg ggaatgcgca ctgttgttgt
     2401 ccacagggcc gacaggctga ccgggccgtg ggaaggaaag cttgtttttc aggatctcgg
     2461 agtagcccag ggaggaatga cagatacccc tgacggcaga tggtttgcat accttttcag
     2521 ggattacgga tccgtcggac ggataccata tattattccc atgacatggg agaacggatg
     2581 gcctgtaatc ggcactgacg gcaaggttcc tgaaatgctc gatcttcctc ccggcaaggg
     2641 actgatacct ggaatagttg caccggatga attcaaaaga aaaaagggtg atccgcctct
     2701 ccctcttgtc tggcaatgga accacaatcc cgacaactcc cgctggtcag tgactcagag
     2761 aaagggattt ctcaggctga ctgcagggcg aattgactct gttttcacac aggcgaggaa
     2821 taccctgact cagcgcacaa tcggtccgga gtgttccgga tcaacgctta tagatgtttc
     2881 aggtatgaag gaaggtgatt ttgcagggtt agccctcctg caaaaaaagt acgggatcgt
     2941 cggggtaaaa tacagcaacg ggatcagatc ggttgtcatg gtcaatgcac agtcaggcag
     3001 ccctgaagaa attgaaagca taccgctgac gcgaaagaag gtttacctga aggcagaatg
     3061 tgacttcact gaccgcaggg atttggctta cttttactac agccttgacg gcaagacatg
     3121 gaaatcaatt ggcaatcagc tgaaaatgga atattcaatg cctcatttca tgggataccg
     3181 gtacgggctg ttcaattatg ccacccggga gcctggcgga ttcgtcgatt tcgactggtt
     3241 ccgcataacc aatgaaagaa ctgaaagtaa ataaactcaa ccagaaacca aaacagtgac
     3301 aaaaaccaac ctaaaccaca gcagataccc ctggaagagg tcgatattca tggtcccctt
     3361 tctgggattt ttgtttggca gtgtaatact taacggtcag cttctcaggc tgaatgagaa
     3421 gggatatttt gaagcgcgcg gagtgaatat actcgtttac agcaaccagt acacgggatt
     3481 ttttttcgat gaaaagactg cgggaattga aatcatacaa cagggagtaa ggacttccac
     3541 tggcggagca gtaagactgc agaacactcc tgaacagtgg gacctcatac ctgcggtaac
     3601 aggacgcaag gttgatgaag caaacaaaac cataaccaca gagctgaact acaaggactt
     3661 cgatttcaat tccaggatca gtgttactgc cagggacaac ggtgtggaga tcaacgttta
     3721 tcttgacaga ccgttgccgc gggagcttga gggtaatgcc ggcttcaatc ttgaattcct
     3781 cccttcggcg tacttcgaga agacctacct ggttgacggt aaacccggta atttcccgag
     3841 atacccttct ggcaacacaa gaatagaatc ttcagacaaa aagattcctc aatatgcagg
     3901 acataccaca tttgatgaca gaggtctcgg tgaatttatt gtccccctgc cccttgcaac
     3961 aggcaaaacc ctgacgctgg cacctgatga ccctgccaga atggtgataa tcaaaggaac
     4021 agaaacagac ctgatgcttt atgacggtcg aaacctcgca caaaacggat ggtttgttgt
     4081 gcgcagcctg ctccctgcaa acaaaacggg gaaggttctt acgtggtacc tggaagtgaa
     4141 ttccgtgcct gactggacaa gggagccggt tataggattc tcgcaggcag gatatgtacc
     4201 cgccggtgag aaggtggcag taatagaact tgacatgaat gacacaccaa tgaattcggc
     4261 acaggttttc caggtcactg ctgatggcag gtcagttgag aaattcatcg gtcccgtgaa
     4321 gatatggggt aaatatttgc gatataatta tgcccggttt gatttttcct cagtcaggga
     4381 accaggcgtt tattacattt catacggtaa tcataaaacg aatccgttcg ttatatcccc
     4441 tgacgtattt gaaaatgtat ggcatcctac tcttgatgtc tggtttcccg ttcagatgga
     4501 tcatatgact gttaacgaag cctaccgcat ctggcacgga gcttcctacc ttgatgattg
     4561 tctgcaggcg ccactgaatc accagcattt cgacggatac aggatgggcg acactaccga
     4621 tacgagattc aggccccttg aacgtattcc gaacatggca gtcggcggat ggtttgatgc
     4681 cggtgacttt gacattcaga ccggttcaca taacagcgtt atcacaaggt ttgttgaggc
     4741 atgggaagaa ttcaggcctg acagggatca gactttcata gaccaggaaa caaggtacgt
     4801 tgacatccat cgtcctgacg ggaagccaga tatcctgcaa cagattgagc atggaacgct
     4861 gaacctggtt gcacagtgtg aaaatatcgg ccaccctgtg acaggaatca tagttcccgc
     4921 catgcatcag tatcatcacc tcggagatgc aatgacggaa acagacaatc taccttataa
     4981 tccggccctt aagccctatg agaccgacgg caggtcgagc ggtacccctg atgaccgctg
     5041 ggccttcaca acccgtcagc catatcttga ttattccacg gcagccaccc ttgcggctgc
     5101 cagtcgcgcc ctgagaggtt ttaacgatga ccttgcagag agaagcctgg ccaatgcaaa
     5161 acggctcgtg gaggaggctg acgaactggg taaaaacaaa accggtaatg accctatgat
     5221 gataatgtgg tcgatgatgg gcatcgaggc tcagcttgaa gctgcactgc aactgtatat
     5281 caccaccggc gagaagaaat atgctgaaag gttcctcggc aagatatggt cttcacttga
     5341 atcgcagcgt ggcggacgcg aaatggcttt tcagggagcc ttttcaggaa tggagcttct
     5401 ccctgcactg aaggcactgc catatatgga tgacagttac aagacaaagc tgaaaggcta
     5461 tgtggtcaaa tacaggaaat cacttgacga aattgaaaaa cagaatccct atgccgtgcc
     5521 tatcaccctc gggtcatggg gtggcaccag ttctgttgtc gcctggggaa ttgcaaacta
     5581 ttacgccttt aaggcattcc ctgatgtgat ggataaggaa tatgtgctgc ggggaatgaa
     5641 ctacgtcctg ggatgccatc cctattcgaa tatatcgttt gttgaggcag tcggaacaag
     5701 gtcaaaaaaa gttgcttacg ggaacaacag ggctgatttc tccataattg caggcggtat
     5761 cgttcccgga ctgatactgc tcaggcctga ttaccttgaa aacaaggatg actggccatt
     5821 cttctggggt cagaatgaag ttacgatggc cggcagcgcc gactatctct tccttgccaa
     5881 agctgccgca gaaatagcaa ataattaaaa tacccggcca gagaaatatt aacagaaaca
     5941 aacagataaa taatgagata cttaaaatta tcaatcgcta tcaccgctat ggtggcaggc
     6001 acattggcct attcgcaaaa tcctgtcatc cgtgaccagt tcaccgctga tccgacagcc
     6061 agggtcttca acgggaaaat gtatcttttc ccctcacatg acatccctgc gccggaaagt
     6121 ttcccgcgaa aagactggtt ctgcatggca gattaccatg tcttctcctc atcaaacctt
     6181 actgactgga ccgaccatgg cgtcattgta agccaggata aagtcgattg ggtcaacccc
     6241 acatcgttca gcatgtgggc gcctgactgc attgaaagaa acggcaagta ctatttctac
     6301 ttcccagcca acgttaagac cggccggggc ttcggaatcg gggtagcaat agcagataac
     6361 ccggagggac catatattcc acagcctgag cctattgcaa aggcaagggg cattgacccg
     6421 agcgtgttca ttgacaagga tggccaggcg tatatctatt attcgatggg cagaatcttt
     6481 gtcgccaaac tgaaagagaa catgaccgag ctggattctg atccggttgt tgttggtgat
     6541 ctgccgacaa aaggattggt tgagggaccc tttttcttcg agaggaacgg aatatattat
     6601 atgacctatc ctcacgtgga aaatacagtt gaaaggcttg aatatgcgat gggtgacaat
     6661 cccctcgggc catttaaggt gacaggggtc attatggacg aaaccccgat ggggacctgg
     6721 actaatcacc actcattggt gcaatacaat gaccagtggt atctctttta tcataacagc
     6781 gcctactcgc cgaagtttga caagaacagg tcagtctgca tagacagcct gttcttcaat
     6841 gaagacggga ctatcaggaa ggtgattcca accaaccgcg gagtaggaat taccagcgca
     6901 caaaccacaa tcgagattga tcgcttcagc caggtaagcc cggcgggagt aactgttgcg
     6961 ctcattgata caataaatcc attccgggga tggacagccc aattgagcgg accaggcagc
     7021 tggatctcat acaatgcagt tgacttcgga aacaaaaaac tcaaatcagt gcgggtcaat
     7081 gcctcatcag tgacaggagg gattattcag atccggctcg ataatccggc cggcctgctc
     7141 cttgcggaga ttgaaattcc ggggaacacg gcgctgagca atgtggatgc aaaactgaaa
     7201 aagtacagta aaggaactca taaccttttt gttgtactga aagatggaaa tccggtgaac
     7261 attgactggg ttcagttcag gaaaaagtca aagtgaattc agtaaatata aaactacccg
     7321 aaatccgaaa aaattatgaa atcactcatt accggttctc tcgcagcatt gctgatgttt
     7381 tctgcctgtt ccaccagtgg gaaagtaagc ccggcaacaa aagaaatggt tctctggtat
     7441 gacaaaccgg cagggaaagc atggctcgat ggtttgttca taggaaacgg ctatatggga
     7501 gccaatgtat tcggaaaggt tcagaatgag cggatagccc tgaatgaatc aaccttctgg
     7561 tcaggcagac cgcatgatta caatgacccg gatgcatata agtatttcgg gcagataagg
     7621 gatctcatgt ttgctggaaa gttcagggaa gcagaaaaac tggcagacga acacttctat
     7681 gggaagccgg gtgctcagca ggcctaccag cctgtgggcg accttcttct cgatttcaag
     7741 gttggcagtg attctgttaa ggattactac cgcgaactgg atatggaaac aggcatcgtc
     7801 aggatcagct acaccgacgg tgatgtgaaa atgacccgtg aagtctttat gtcatatcct
     7861 gaccatgtaa tggtaatgag gatatcggca gacaaaccgg ggaaggtatc tgttgaggca
     7921 aaactcagca gtccgttcgc cgaaaaaaca aaggtaacgg ataacagact gactctcagc
     7981 ggcacatgga aatatcttcc cgaagagatc agctggcttg ttgcgaaggt cgaaggtccg
     8041 gg
//
LOCUS       DAOPXU010000118         7842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_11148_length_7842_cov_11.515603, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000118 DAOPXU010000000
VERSION     DAOPXU010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7842
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11148_length_7842_cov_11.515603"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /gene="crtI"
                     /locus_tag="PKK03_10350"
     CDS             <1..1371
                     /gene="crtI"
                     /locus_tag="PKK03_10350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HNX84842.1"
                     /translation="GRLNQLTSGGFTFDMAPTFFSMSYHFDEFVRDAGIEMPFRFVQI
                     DPLYRVNFRGSDRWYTIYRDLDRLAHQFEDIEPGFRDRMARFLASSGALFDDIEKGVL
                     AKNFRSLPDFMMQMTKLPVKHVPKVMRSVWSEMSRYFTSYEVRVIFSLVSFFLGATPF
                     DTPAVYTILSYTEMVHDGYHNVEGGMYRIVDGLLREAEKAGIKINYNVTINGFDGSGR
                     RNRAFIDTDGRRWEGDIFVINADAAMFRGRVFRRPAFTDEKLDRMQWTLAPLTIYLGV
                     KGKMENIHHHNYFLGDRDYEPYSKGIFRNKVSLDKPYYYVNANAIFNPQSAPPGCENL
                     FILVPVPDLRFKPDWSDRERIADAVIKDLSARTGHNVEANLMTRVVLDPVDWQNMFGL
                     YRGSGLGLGHKMTQVGGFRPSNRDEMFSNVWYTGASTVPGTGLPMVIISSKLTVQRID
                     NEAGTL"
     gene            1352..>1836
                     /locus_tag="PKK03_10355"
     CDS             1352..>1836
                     /locus_tag="PKK03_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HNX84843.1"
                     /translation="MKPALYDTISLRCSKVVTTGYSTSFSLGIRTLAVRHRPAIYAIY
                     GFVRLADEIVDTFFGHSQREMLDRFRQDTAEAISGGVSPNPVLHSFQWAVNTYNIDNE
                     FIEAFLCSMEMDLEQKNHDRHSYNQYIYGSAEAVGLMCLRVFCHDDPRAFDRLREPAR
                     RL"
     assembly_gap    1837..1936
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2083..3552
                     /gene="crtI"
                     /locus_tag="PKK03_10360"
     CDS             2083..3552
                     /gene="crtI"
                     /locus_tag="PKK03_10360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HNX84844.1"
                     /translation="MKTKAAVIGAGIGGLASAIRLAVKGYDTHLYEKSSRTGGKIGEM
                     RLAGCRFDTGPSLFTLPELVDELFTLAGEDPRGHFRYVPLVSSCRYFWDDGTLLNAWT
                     DRQRFAEEAERVTGEPRENILGFLDECAELYDLTAGIFIFSPFYQLENFRKPESLNVA
                     RNFRKLNAFSTMHGIISRRFRSPKLIQLFDRYATYNGSSPYRAPGTLNVIAHLEHNMG
                     AFFPEKGMREIVDALELLAGRCGVTIHTGEEVVSVVKQGKIVSAVETTAGVKPFGVVV
                     SDVDVIPFYRKLLKEETYARKQERQERSTSALIFYWAMNRRFEELDLHNIFFSSDYPG
                     EFRALRKGEVSADPTVYLFASSRAVSTDAPEGKDNWFVMINVPYDRGQDWKGVVSVAR
                     GNIIQKLGKRLSCSIEEAIGAEQVLDPPLIESLTASHRGALYGSSSNSRFAAFRRHPN
                     VRNRYENLWFVGGSVHPGGGIPLCLASAKIACDMIPAAK"
     gene            3549..4238
                     /locus_tag="PKK03_10365"
     CDS             3549..4238
                     /locus_tag="PKK03_10365"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HNX84845.1"
                     /translation="MTALKPRLLKSTEILLWVYGVGLAGMLLPFSRELFKLITPLNLL
                     FAAVFLFWGKLPARRVIFTGLFIAVTSFFIEAAGTETGKIFGVYIYGQTLGPTLLNTP
                     LIIGLNWFLLIYCTNVISRQLWERLPHDNTRRHGGVIKSLFVIFTGSLLMVAYDLFLE
                     PAAMRLDMWSWECKVIPLKNYLGWFFFSAVFHAVTRVWGEEEINSRALPLFAVQMGFF
                     AAIDLFYLLAA"
     gene            complement(4220..4954)
                     /locus_tag="PKK03_10370"
     CDS             complement(4220..4954)
                     /locus_tag="PKK03_10370"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNX84846.1"
                     /translation="MDNQDLRLELKAIDTSSLVDKVEMRLIEVFLYKELKPGDAIPKE
                     TELASIMGVSRTVIRESLNRLKTMGLIESKKHRGTIIKSPDLSVILGKSLIPRILDNK
                     TLMDVFEMRLALEVGMADFIFARKTARDVRELEEIVEIEPDHSDNILFDVEHELKFHG
                     KLYQMTGNNTLEEFQNLLLPAFQHVYNIGLIKVKGKRKKYKSHKELVALLKKGTPDEF
                     REGMRRHLENHFVRILANHASQAANR"
     gene            5276..6913
                     /locus_tag="PKK03_10375"
     CDS             5276..6913
                     /locus_tag="PKK03_10375"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNX84847.1"
                     /translation="MRTLLLFLLLLLTASKCGDSKEATSGFAVSETVAIFSTEPIFPE
                     RTNNDLVSLMFTITGEDESVSLKSIGITFSDESSPQALSALTAWYTGVVPGVPQKEML
                     ATVSEPSLFTLVECQLPVAGGKHTFMFDFRTRANADLAGRLKIESVIFTFSNGSSTSY
                     EAKPEDPVIRFGRIVRAAGQDGADTYRIPGLVTTNEGILVAVYDIRYNNSKDLQEDID
                     IGMSRSTDGGRTWEPMKVIMDMGEYGGLPQNLNGIGDPSILYDHTTGTLWVAALWMSG
                     SAPDKMLWWASQPGMTPQETGQFILVKSTDDGLTWSEPVNITSQIKDTAWQLLLQGPG
                     RGITMDDGTLVFPAQFKADIGTKALDGGQYTCHSTVVCSRDGGNTWQIGTGAKSNTTE
                     AQVVQLADGTLMLNMRDDRNRSDKGDTNGRAVAVTGDLGRTWTAHPSSNSALPEPNCM
                     ASLISAKIKIAGADKNILFFSNPDDRNSRIRMTIKASTDQGLTWPEENQVELFGPSGY
                     GYSCLTMVNETQVGILYEGVKELYFQIIPAADFFGDK"
     gene            6928..7341
                     /locus_tag="PKK03_10380"
     CDS             6928..7341
                     /locus_tag="PKK03_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84848.1"
                     /translation="MNLLAKKWLLLILISLFTGGITYPAPASEKTSLIPAPQIISRSA
                     ENFRVSQTGLSDDSRIRKAPRSPEPVSAERKGDMVIVSFRYSGRLKTSVRLPLREVEV
                     SESGHDFKAATARIRGNRLEINAPGAMAVRYGWKP"
     gene            7412..>7842
                     /locus_tag="PKK03_10385"
     CDS             7412..>7842
                     /locus_tag="PKK03_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNX84849.1"
                     /translation="MKIEKLAGLIAAPFTPMYPDGEINSGIIEKYHRLLVSNGVVGAF
                     INGSSGEGVSITNHEKLIVAEAWAKASSTSNLKVISHLGGTCYRDCIELAGESEKMGL
                     YAVAVTSPSYFKPANVKMLAEFFIRVAESVPEMPVYYYHIPV"
BASE COUNT         1891 a         1872 c         2112 g         1867 t
ORIGIN      
        1 ggaaggctca accagctgac ctccggaggc ttcacctttg atatggcccc cacctttttc
       61 agcatgagct atcacttcga cgagttcgtg agggatgcgg gcatagagat gccgttccgg
      121 ttcgttcaga ttgatcccct gtatcgcgtg aacttccggg gtagtgacag atggtacacc
      181 atataccgtg acctcgacag gcttgctcac cagtttgagg atatcgagcc gggcttccgt
      241 gaccggatgg cacggttcct tgcctcatca ggggcgctgt ttgatgacat cgaaaagggt
      301 gtactggcca aaaacttcag atcgctgccc gacttcatga tgcagatgac gaagctgccg
      361 gtgaagcatg tcccgaaagt gatgcggtca gtgtggagcg agatgagccg ttattttacc
      421 tcctatgagg tgagggtcat cttctccctg gtatcattct tcctcggggc caccccgttt
      481 gacaccccgg ccgtgtatac catcctgagc tacaccgaga tggtgcatga cggctatcac
      541 aacgtggagg gcggaatgta tcgtatcgtg gatgggttgc ttcgcgaagc tgaaaaggcc
      601 ggaattaaga ttaactacaa tgtcaccata aacggcttcg acggttcggg caggaggaac
      661 agggcattca ttgacactga cggcaggcgc tgggagggag acatattcgt tataaacgct
      721 gatgctgcta tgttccgggg aagggtgttc cgccggccgg cattcactga tgaaaagctt
      781 gaccggatgc agtggacgct ggctcctctt acaatctacc tgggtgtaaa gggaaagatg
      841 gagaacatcc accatcataa ctatttcctc ggggacagag actatgaacc ttattcgaaa
      901 gggatcttca ggaataaagt ttcacttgat aagccctatt actacgttaa cgccaacgcc
      961 attttcaatc cgcagagtgc acctcccggc tgtgagaacc tgtttatcct tgtgccggtg
     1021 cctgacctga ggtttaaacc cgactggagt gacagggagc gcattgccga tgcggtgata
     1081 aaggatctgt cggcccgtac tggccataac gtagaggcca atctcatgac gcgcgtggtg
     1141 cttgacccgg tcgactggca gaatatgttc ggtttatacc gcggcagcgg gctcgggctg
     1201 gggcacaaaa tgacccaggt ggggggattc aggccctcaa acagggatga aatgttttca
     1261 aacgtctggt atacaggcgc ttcgactgtt cccgggacag gtctcccgat ggttataatc
     1321 agctcaaaac tgacagtaca gagaatagat aatgaagccg gcactttatg acaccatatc
     1381 actgaggtgc agcaaggtgg ttaccaccgg ttacagcacc tctttctcgc tggggatccg
     1441 taccctggca gtaagacatc gtccagccat ctacgcgatt tacggttttg tcaggctggc
     1501 cgacgagata gtggacacct tcttcggcca cagccagagg gagatgctgg accggttccg
     1561 gcaggatacg gcagaggcga tctccggggg ggtcagcccg aatccggtgc tacactcgtt
     1621 ccagtgggcg gtaaatacct ataatattga taatgagttc atagaggcat tcctctgcag
     1681 catggagatg gaccttgagc agaagaacca tgatcgccac agctataatc aatacattta
     1741 cggctcagcc gaagcggtgg gactgatgtg cctgagggtt ttctgccatg atgatccccg
     1801 ggcatttgac aggcttcgcg aacctgcccg caggctnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnggcg ctctttgcca ggatcaggaa aacgccgccg gaagctgtta
     1981 ccggacagcg tttccgcatt tccaacacca ggaaattatg gcttcttctg aaggtgattg
     2041 tgaaggacgg attaggattg atataacaga ccacacagag cgatgaagac aaaggcagca
     2101 gttatagggg cgggcatcgg aggcctggca tcggcaatta gactggccgt aaagggttac
     2161 gacacccacc tttatgaaaa gagcagccgt accggaggca agatagggga gatgagactt
     2221 gccggttgca ggtttgatac cggcccctct ctgttcaccc tccctgagct ggttgatgaa
     2281 ctcttcaccc ttgccggtga agacccccgc gggcatttca ggtatgtgcc actggtatcg
     2341 tcgtgcaggt atttctggga tgatggaacg ctgctcaacg catggactga cagacagcgc
     2401 tttgcggagg aagctgagag agtcaccggt gagccgcgtg aaaacatact gggtttcctg
     2461 gatgagtgcg ccgaactgta tgacctgacg gcagggatat ttattttttc acctttttac
     2521 cagcttgaaa acttcaggaa gccggagtcg ctcaacgttg cccgcaactt caggaagctg
     2581 aatgccttct ccaccatgca cgggattatc agcaggcggt tcagaagccc gaagctgatc
     2641 cagctctttg accgttatgc cacttacaat ggctcaagtc catacagggc gccgggaaca
     2701 ctgaacgtga ttgcgcacct ggagcacaac atgggcgcct tcttccctga aaaggggatg
     2761 agggagattg tagacgcact tgagctgctg gcaggcaggt gcggagtgac aatacatacc
     2821 ggcgaggagg tggtttcggt ggtgaagcag ggaaagatcg tcagtgcagt tgaaaccaca
     2881 gcgggagtaa aaccgtttgg tgttgttgta tcggacgttg atgtgatacc gttttaccgg
     2941 aagcttctta aggaggaaac ttatgcaaga aaacaggaga ggcaggagcg gagcacctcg
     3001 gcgctcatct tttactgggc aatgaacagg cggtttgagg agctcgacct gcacaacata
     3061 ttcttctcat ctgactatcc gggtgaattt cgcgcactgc gaaagggaga ggtctccgct
     3121 gacccgactg tctatctgtt tgcatcatcg agagcggtta gtaccgatgc tcctgaaggg
     3181 aaggacaact ggttcgtaat gatcaacgta ccgtacgaca gagggcagga ctggaagggg
     3241 gttgtaagtg tggctcgcgg aaacatcatt cagaaactgg gaaaacggct cagctgcagc
     3301 attgaggagg ctatcggggc agagcaggtg cttgacccgc cgctgataga gagcctgacg
     3361 gcctcgcacc gcggcgccct ctacggcagc agctccaaca gccggttcgc ggctttcaga
     3421 cgccacccga acgtaaggaa caggtatgag aacctgtggt ttgtcggtgg ttctgtccac
     3481 cccggcgggg gcatacccct ctgccttgca tccgcaaaga ttgcatgtga catgatacct
     3541 gcggcaaaat gacagcactg aaaccacgat tgttgaaatc gaccgagatc cttttatggg
     3601 tttacggcgt tggactcgcg gggatgctgt tgcctttcag cagggagctt ttcaagttga
     3661 taaccccgct taacctgctg tttgcggcgg tgtttctttt ttgggggaag ttgcctgcca
     3721 ggcgggtgat tttcacggga ttgttcattg cggtgacttc cttcttcatt gaagccgccg
     3781 gcaccgaaac cgggaagatt ttcggagtat acatctacgg acagactctc ggaccaaccc
     3841 tgctgaacac accattaata atcggcctca actggttcct gctgatctat tgcacaaatg
     3901 tcatcagccg gcagttgtgg gaacgattac cgcatgacaa tacacgccgg cacgggggag
     3961 taataaagtc gctgttcgtg attttcacgg gctcgctgct catggttgca tatgaccttt
     4021 tcctggagcc ggcagcaatg aggcttgaca tgtggtcatg ggagtgcaag gtgattccgt
     4081 tgaagaatta cctcggctgg tttttcttct ctgccgtgtt tcatgcggtg accagggtct
     4141 ggggagagga ggaaatcaac agcagggcgt tgcctctctt cgcggttcag atgggcttct
     4201 ttgcagcgat agatttattt tatctgttgg cagcctgact tgcgtggttt gccagtatcc
     4261 tgacaaagtg gttttcaaga tgtcttctca ttccctcgcg gaactcgtca ggggtgcctt
     4321 tcttcagaag tgccaccagt tccttgtgtg acttgtattt cttccgtttc ccctttacct
     4381 tgatgagccc gatgttgtaa acatgctgga atgccggtaa cagcaggttt tgaaattctt
     4441 caagagtgtt gttgccggtc atctggtaca gtttgccgtg gaatttcaat tcatgctcca
     4501 cgtcaaacag gatattgtct gagtgatcag gttcgatctc gactatctct tcaagctccc
     4561 ttacatccct ggcggtcttc cgtgcgaaga tgaaatctgc catgccgact tccagggcga
     4621 ggcgcatctc gaaaacgtcc atcagtgttt tgttatcaag tatcctgggg atgagtgatt
     4681 tacctaggat tacggaaaga tcagggcttt tgatgatggt tcccctgtgt ttttttgatt
     4741 cgatcaggcc cattgttttg agcctgttca gtgactccct gatgacagtc ctgcttaccc
     4801 ccatgataga ggcgagttct gtctccttgg gtatggcgtc accgggttta agttccttgt
     4861 agaggaaaac ctcaatgagg cgcatctcga ccttatcgac gagactgctt gtgtcgattg
     4921 ctttcagctc aagtcttaag tcctgattat ccattattta tccaatagat actaaagtat
     4981 tacaaaaaaa gcaaaaataa aggagaaatc cttgcatgtg gaattttaaa attaaatttg
     5041 tattattcta tgtattacat aaagcaatta cttcttcaat tctaatccca taaccaactc
     5101 aatttttgaa aagaaaattc tctttcactc gcgggctgat catgcttttt ccttaacagg
     5161 tgctcaacag aagccccctg aaggccggga aagcatccgg gaaataatga cggtactggg
     5221 aagggccggg atactgcctg tttttttgaa tgattttttt caaagatttg tatttatgag
     5281 aacactgctc ctttttcttt tacttctact gacagcttcg aaatgcggag actcaaagga
     5341 ggcgacttcc ggatttgccg tcagcgagac cgttgcaatc ttcagtacag aaccgatttt
     5401 tcccgaaagg actaataatg accttgtcag cctgatgttc acgatcaccg gtgaggacga
     5461 atctgtctcc ctgaaaagca tcggaattac cttctcagac gaaagcagcc cgcaggcact
     5521 gtcggcactg accgcatggt atacgggtgt ggttcctgga gtaccgcaga aagagatgct
     5581 ggcaactgtc agtgaacctt cactctttac gctggttgag tgccaattgc ctgttgcggg
     5641 aggcaagcat actttcatgt ttgatttcag gacaagagcc aacgctgacc ttgccggaag
     5701 actgaagata gaatctgtaa tctttacatt cagtaacggg tcatcaacca gttacgaggc
     5761 aaaaccggaa gatcctgtta ttcgctttgg aaggattgtc cgtgcagcgg ggcaggatgg
     5821 agcagatacc taccgcattc cgggacttgt gacaacaaat gaaggaatcc tggtggctgt
     5881 atatgacatc aggtacaaca acagcaagga ccttcaggag gatattgata taggtatgag
     5941 ccgaagtaca gacggagggc ggacatggga gccgatgaag gtgataatgg atatgggtga
     6001 atacggaggt ctgccacaga acctgaacgg cattggtgat cccagcatcc tgtatgatca
     6061 tactaccggt acactatggg tggcagcgct ctggatgagc ggttcagcgc ctgacaagat
     6121 gctctggtgg gcttcgcagc ctgggatgac cccgcaggag accggccagt tcatcctcgt
     6181 gaagagcact gatgacgggc tcacatggtc tgaaccggtg aatatcacct cacagatcaa
     6241 ggatactgca tggcaactgc tccttcaggg acctggccgg ggtattacaa tggatgacgg
     6301 aacacttgtt ttcccggccc agttcaaggc agacatcgga acgaaggccc tggacggggg
     6361 gcagtacaca tgccactcga ctgttgtctg cagcagggac ggagggaata catggcagat
     6421 tggtaccggc gcaaaatcaa atacaactga agcccaggtg gtgcagcttg ccgacggcac
     6481 acttatgctt aacatgcgtg atgacagaaa caggagcgac aaaggtgata caaacggcag
     6541 ggcagtagcc gttaccggcg acctgggcag gacatggacc gctcatcctt cttcaaattc
     6601 tgccctccct gagccgaact gcatggccag cctcatctcg gcaaagatca aaattgccgg
     6661 agcggataaa aatattctct tcttttccaa tccggatgac aggaacagca ggatcaggat
     6721 gaccatcaag gccagcactg accagggatt gacctggccg gaggaaaacc aggtggaact
     6781 gttcggtcct tccggttacg gatattcatg cctgacaatg gttaacgaaa cccaggtcgg
     6841 tatcctctat gagggggtta aggaactcta ttttcaaata attcctgcag ccgatttctt
     6901 cggggataag taacctgcct ctccctgatg aatctcctcg caaaaaaatg gctccttctg
     6961 atcctgatca gcctgtttac aggcgggatc acttacccgg caccggcgtc agaaaaaaca
     7021 tctctgatac ctgcgccaca gataatcagc aggtccgcag aaaatttcag ggtcagtcag
     7081 accggtttgt ccgacgattc gaggatcaga aaggctcccc gcagcccgga acctgtctcc
     7141 gctgaacgaa agggggacat ggtcatcgtc tcattcaggt attcaggcag gctgaaaacc
     7201 tcagtcagat tgcctctcag ggaagtcgag gtatccgaaa gcggtcatga cttcaaggcg
     7261 gcaactgccc gcatccgtgg caaccgtctg gaaatcaatg ctccgggagc aatggctgta
     7321 agatatgggt ggaaaccata atcagaagga aatttaaccg gtgaatcagg tttgccggtc
     7381 tcaatattca gacttatgat aaaatgacat tatgaagatt gaaaaacttg ccggcctgat
     7441 tgccgcaccc ttcactccga tgtatccgga cggggaaatc aattccggca taattgagaa
     7501 atatcacagg ctgcttgtct ccaacggagt ggtgggtgct ttcataaacg gctcatcagg
     7561 cgaaggtgtc tcaatcacga accacgagaa gctgattgtt gcagaagcct gggcaaaagc
     7621 ctcttccacc agcaatctga aggtcataag tcacctcgga ggaacatgct acagggactg
     7681 tatcgaactg gccggggagt cggagaagat gggactttat gcagtggcag tgacatctcc
     7741 ctcgtacttc aaaccggcaa atgtgaagat gcttgcggag ttcttcatca gggtggcgga
     7801 atcagttcct gaaatgcccg tttattacta tcatatcccg gt
//
LOCUS       DAOPXU010000119         7808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_11214_length_7808_cov_12.420611, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000119 DAOPXU010000000
VERSION     DAOPXU010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11214_length_7808_cov_12.420611"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PKK03_10390"
     CDS             <1..711
                     /locus_tag="PKK03_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016110865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNX84850.1"
                     /translation="EIELDPNETIVAEAGTMVYMEQGITYETRMGDGASPDRGVMDKL
                     FQAGSRILTGESLFLTHFTNKGSMRSKVAFAAPYPGSIIPIDLRNVNGSLIVQKDGFL
                     CAAFGTKLSVTLNRKLGAGLLGGEGFILQRVEGDGKMFVHAGGTVIEKQLSGETLRID
                     TGCIVAYEPSLDFDIETTGSLKTMVFGGEGIFLATMRGTGKVWLQSMPIRKLIRALSP
                     MSSNRGKEAQGLLGNLFQ"
     gene            complement(797..2599)
                     /locus_tag="PKK03_10395"
     CDS             complement(797..2599)
                     /locus_tag="PKK03_10395"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HNX84851.1"
                     /translation="MPEQFNRNNLRESSSPYLRQHAGNPIWWQEWTSETLAYAASEKK
                     PLLVSVGYATCHWCHVMAREAFSDPETAEYVNDHFISIKVDREQRPDIDQYMMNFIQA
                     QTGSGGWPLNVFLTHDLKPVHALTYAPLQTSGYRYSFLHIARAVIDFLEKQADRIVPF
                     SLTEQEAPHADSERLTESLSEYFDAEDGGFGQGQKFPPHSTLLYMLYRLSVSPDETLS
                     RMVTTTLDAMMMRGLNDHLQGGIYRYCVDREWTIPHFEKMLYDQAMSLWSYSLAYRVT
                     GRESYLTMAEKITRGLHETFAADGMYVSAFNADTDHKEGATYLWSLKELAALLTREEL
                     SRFRDVYVVTEDGNFEGLNHLVRKNAGRVDDIEEKLLAARKQRSQPFRDDKILSGLNA
                     LTAVAFIQAGRNLDMPGLEAEAAELTRKLLETFRDGSSLAHSFYDGVMLRQSFLSDAA
                     AMLLAVSMLYETDESWGETMDSMAEYVRSFRDGSGHWIESDADDFQKIYASWFDHPVP
                     SAVSLAETALTRVALLTGGEVTPAVHRRPYQSDFYNINVLLTEDLFHLYTTRDPLSWS
                     SIPVNSLQRRGEPETVCYGRTCQVLRNNAAGSQQ"
     gene            complement(2622..3317)
                     /locus_tag="PKK03_10400"
     CDS             complement(2622..3317)
                     /locus_tag="PKK03_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4918 family protein"
                     /protein_id="HNX84852.1"
                     /translation="MRFFADRIIEFNRTVEFTGSLPDGINIMNPFRGEDDYIFQVSSA
                     FYRKFYSDNSPRRLILGINPGRFGAGFTGVPFTDTKRLTAECGIPYSGRQTHEPSSVF
                     VYEMIKAYGGQEKFYSQFYITSVCPLGFTQSSPGGREKNYNYYDRPDLTAAVYEFMVE
                     NIRRQIALGVMTDTCFCLGTGKNEKFLIALNAKHNFFGRIIAVEHPRFIMQYRSKRMH
                     EYIAKYLEAFRQV"
     gene            complement(3387..3953)
                     /locus_tag="PKK03_10405"
     CDS             complement(3387..3953)
                     /locus_tag="PKK03_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNX84853.1"
                     /translation="MADIQTIYQETIKFAAEKHGAQKVKGAKIPYVVHLSNVTMEIFM
                     AARKTHGFDLGYALQVALLHDTIEDTETTRQELEMVFGKDIADAVWALTGDPKLLKEY
                     RLSDSLAKIRQCPREVGAVKLADRITNLQAPPKSWSKGHISDYLFDAKQILKALHGSN
                     EYLEARLAAKIEEYGKYLKVERSESLKV"
     gene            4185..5030
                     /locus_tag="PKK03_10410"
     CDS             4185..5030
                     /locus_tag="PKK03_10410"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX84854.1"
                     /translation="MKTLRFFLAIAVALSLQLNVCGQDYRFPGNAGNLTTVNRESGIT
                     SPVTVTVIYDNYVHSEGMKPDWGYSILIEGLDKTVLFDTGTKPAIFSSNFKLTGIDAG
                     SIDILAFSHEHLDHVGGLSAFAEMKTGIPAVIPHSFTPPVVSSIVNAGYIPLLVRDAA
                     MICNNLYTSGEFDFGIAEQCLVLDTRAGLVVMTGCAHPGIVRMLSEIKQTFNKNIVAV
                     FGGFHLMDKSKAGTEEIIAGMKSLGIVRCGATHCTGEKQIDMFREAFGENYFELGAGN
                     RVIFN"
     gene            5099..5452
                     /locus_tag="PKK03_10415"
     CDS             5099..5452
                     /locus_tag="PKK03_10415"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HNX84855.1"
                     /translation="MGGTVLYDGECSFCNSQVRFIRERDTRNRFRFVPLQSDEGRELL
                     RKAGLPENELDSVVYDRSGRQYLRSSAVLGILRDMGRGWSLLYSLVIVPACIRDVFYR
                     LVARNRHRLTPRSRS"
     gene            5530..5823
                     /locus_tag="PKK03_10420"
     CDS             5530..5823
                     /locus_tag="PKK03_10420"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HNX84856.1"
                     /translation="MILSTCGLKCDECEFYNKTCSGCRNVMGATFWAIEMMPAKVCPL
                     YDCAVNQRGHKDCGECAELPCATFLQMKDPATSDEEHQRMIGIRVDLLRATAN"
     gene            5847..6584
                     /locus_tag="PKK03_10425"
     CDS             5847..6584
                     /locus_tag="PKK03_10425"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX84857.1"
                     /translation="MEEITRKSLLYRSGLGFWCINHVHGCSHGCKYPCYAWMMAHSHG
                     RVGTYAEWCRPSLVVNAEALLSKELERMKSKPGNIHLCLTTDPFMYGYPEVTSLTLRM
                     IELINSYGISCSTLTKGVTPVDLADSIRFPEQNSYGISLVSLSEEFRKKWEPGAAPYN
                     ERIGALRELHDLGCSTLVHIEPYPTPNIIEQDLRELLDAVDFADEIWFGSWNYNNLAK
                     QYPGFREFYDEQSAILSRFLSTRQDSL"
     gene            6772..>7808
                     /locus_tag="PKK03_10430"
     CDS             6772..>7808
                     /locus_tag="PKK03_10430"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNX84858.1"
                     /translation="MRSLLQKYLSALVLMSAVSSVASGQPWYVATTGSDSNAGTLSSP
                     FKSIEKAVSVVKGGETIYLRGGTYNLTATITLSKSGAENTVISVIAYPGEVPVLNFSA
                     QTQSSGNRGLVLTGSWWHIKGISITGAGDNGMIISGGNNNLIDQCSFYRNRDTGLQID
                     NGASDNQVRNCDSYFNADPPDNDDADGFAPKLTAGSGNSFYGCRAWRNSDDGWDGYLR
                     GTNDITTTLEYCWAFENGFLENGTDAGENANGNGFKIGGSDDKMLMHNFILKNCLAFG
                     NKSKGFDQNNNRGSMTLYNCTGHNNGADNYRISQALAAGKALTLKNCAELGGKVSIGT
                     FAVQEKNSWLTP"
BASE COUNT         1881 a         2107 c         2023 g         1797 t
ORIGIN      
        1 gagatagagc ttgacccgaa tgaaacaatc gttgccgagg ccggcaccat ggtatatatg
       61 gaacagggaa tcacctatga gaccaggatg ggcgatggcg catcacctga ccgcggcgtg
      121 atggacaagc tcttccaggc aggctccaga atactgacgg gtgaatcact cttcctgacc
      181 cacttcacca acaaaggttc aatgaggagc aaggtggcct tcgctgcccc ttatccggga
      241 tcaatcatcc caattgacct caggaacgtc aacggaagcc tgatagttca gaaagacggg
      301 ttcctgtgtg ctgccttcgg cacgaagctg tcggtcacac tgaaccgtaa gctgggagca
      361 ggactgctcg gcggcgaggg atttatcctg caacgcgtcg agggcgacgg aaaaatgttt
      421 gttcatgccg ggggaacggt gattgagaag cagctcagcg gggagacgct gcggatagac
      481 accggctgca tagtagcata tgaaccctcc cttgattttg acatcgagac caccggcagc
      541 ctgaagacga tggtcttcgg cggcgaggga attttcctag ccacaatgcg cggtaccggg
      601 aaggtgtggt tgcagagcat gcccatccgc aagcttatcc gcgccctctc acccatgagt
      661 tccaacaggg gcaaggaggc gcaggggctg ctcggaaacc tgttccagta gaatttgcga
      721 attgcgattt tgcccgccgg gcatacagca gtgtatactc aagccccctt ttttgcggcc
      781 tgacagaaaa ttatgcctac tgctgactgc cggctgcatt gttccggagt acctggcagg
      841 tcctgccata acataccgtc tccggctcac cgcgccgctg gagggagttg accggaattg
      901 agctccacga cagcggatcc ctggtagtat agagatggaa gagatcctcg gtcagcaata
      961 cgttgatatt gtaaaaatcc gactgatatg gtcttcggtg cactgccggg gtcacctctc
     1021 cgcccgtgag cagcgccacg cgtgtcagcg ccgtctccgc caggctcact gccgaaggca
     1081 cagggtggtc aaaccatgat gcatatatct tttgaaaatc atcggcatca gattcaatcc
     1141 agtggccgga cccgtcgcgg aatgacctga catattccgc catggaatcc atcgtctctc
     1201 cccatgactc atcagtctcg taaagcatag agactgccag cagcattgcc gcggcatcgg
     1261 agaggaagct ctgccggagc atcaccccgt cataaaatga atgagccagt gacgagccgt
     1321 cccggaaagt ttccagcagc ttacgtgtca gctctgccgc ctcagcctca agcccgggca
     1381 tatccagatt gcggccggcc tggataaacg ccactgctgt cagtgcattc aggccgctga
     1441 gtatcttgtc atcacggaag ggctggctgc gctgcttccg cgctgccaga agcttctcct
     1501 caatatcatc tacccgcccg gcattctttc tgaccaggtg gttcagcccc tcgaagttgc
     1561 cgtcttctgt taccacatac acatcgcgga accttgaaag ttcctcccgg gtcagcagcg
     1621 cagccagctc cttaaggctc cagaggtagg tggcaccctc cttatgatct gtatcagcat
     1681 tgaaggccga gacatacatc ccgtcagcgg caaaggtctc gtgaaggccc ctggtgatct
     1741 tctctgccat cgtaagatag ctctccctgc ccgtcacacg gtatgccagc gaataactcc
     1801 aaagcgacat cgcctggtca taaagcatct tctcaaaatg aggaattgtc cattcgcggt
     1861 caacgcagta acggtagatg cctccctgca gatggtcatt cagtccccgc atcatcatcg
     1921 cgtcgagcgt cgtggtgacc atccggctga gggtctcatc gggtgagaca gaaagacggt
     1981 aaagcatata aagcagggtg gagtggggag ggaatttctg gccctggccg aagccaccgt
     2041 cttcggcatc aaaatactcc gagaggcttt cagtcaggcg ttcagaatct gcgtgaggcg
     2101 cctcctgctc cgtcaggctg aagggaacaa ttctgtcagc ctgcttctcc agaaaatcaa
     2161 taacagcccg ggctatatgg aggaacgagt accggtaccc tgatgtctga agcggagcgt
     2221 aggtcagcgc atgaacaggc ttaaggtcat gtgtgaggaa aacattcagc ggccatcccc
     2281 cgctgcctgt ctgagcctgt atgaagttca tcatgtactg atcgatgtca ggcctctgct
     2341 cacggtctac cttgatgctg atgaagtgat cattgacata ttcagctgtc tccggatcgg
     2401 aaaaggcctc cctggccatc acatgacacc agtggcatgt ggcgtatccc acagagacaa
     2461 gcagcggctt cttctcagat gctgcatagg caagcgtttc gctggtccat tcctgccacc
     2521 atatcgggtt gcctgcatgc tgtcggagat agggagatga tgactctctg aggttattgc
     2581 gattgaattg ttccggcatt gtgatgtttt tcgaaattta atcaaacctg ccggaacgcc
     2641 tccaggtatt tcgcaatgta ttcatgcatc cgcttcgagc ggtattgcat gatgaacctc
     2701 ggatgttcca ctgctatgat ccggccaaag aaattgtgct tcgcgtttag cgcaatgagg
     2761 aacttctcat tcttcccggt cccgagacag aagcaggtat cggtcatcac cccgagtgca
     2821 atctgcctcc tgatgttctc aaccatgaat tcatatacgg ccgcggtcag gtcaggccgg
     2881 tcatagtaat tgtagttctt ttccctgccg cccgggctgc tctgcgtgaa ccccaggggg
     2941 caaaccgaag tgatgtagaa ttggctgtag aacttctcct gcccgccata ggctttaatc
     3001 atctcataga caaagaccga tgagggttca tgtgtctgac gacccgaata ggggatgccg
     3061 cactctgccg tcagccgttt cgtgtcagtg aatggcactc cggtgaatcc ggcgccgaaa
     3121 cggccgggat tgataccaag aatcagccgc cgcggactgt tgtcactgta aaatttcctg
     3181 taaaatgcag atgaaacctg aaagatgtag tcatcctcac ccctgaaggg attcatgata
     3241 ttaattccgt cgggcagact gcccgtgaac tccactgtcc tgttgaattc aatgatccgg
     3301 tctgcaaaga aacgcatctg gcatttcgca tttggcattt ggcttctcag cagatcgaat
     3361 ttcgcaaatc gcaaatcgca aatccctcag accttcagac tttccgacct ttcgaccttc
     3421 agatacttcc cgtactcttc tatcttggct gccagccttg cctcaaggta ttcattcgaa
     3481 ccatgcaaag ccttgagtat ctgcttggca tcaaacaggt agtcactgat atggcccttg
     3541 ctccagcttt tcggcggagc ctgcaaattg gtgatacggt cggcaagctt gactgctccc
     3601 acctcgcgcg gacactgcct tattttggcc aggctgtcgg agagcctgta ctccttgagc
     3661 agcttcggat ctcccgtgag cgcccagaca gcatcggcaa tatcctttcc gaaaaccatc
     3721 tccagctcct ggcgggtggt ttccgtgtct tctatcgtgt catgcaggag agccacctgg
     3781 agagcataac cgaggtcaaa tccatgggtt ttgcgtgcag ccatgaatat ctccattgta
     3841 acattgctca ggtgaacaac atacggaatt ttagctcctt taaccttctg tgcgccgtgc
     3901 ttttccgccg caaattttat agtctcctgg tagatcgtct gaatatcagc catatatgaa
     3961 acactaattg gaccgggtga agatagtgaa aaaaatgctg acattttaat tatcacaaaa
     4021 agtcattatt ccaaacagtc agtgattttc cggcgaagtt ggctgtgctg acatccttca
     4081 tggctgacat gcactgattt agactccctg agcgggccat ctttttaatc cggtacttga
     4141 catgcttctt ccggtagcct aactttacag ataagtaaat ggccatgaaa acactgagat
     4201 tttttttagc aattgctgta gccctcagcc tgcaactgaa tgtatgcggg caggattaca
     4261 ggttccccgg gaatgccggg aaccttacca cggtgaaccg cgaaagcgga atcacatccc
     4321 cggtcaccgt cacagtgata tatgacaact atgttcattc ggaagggatg aagccggact
     4381 ggggatactc aatcctaatt gaaggacttg ataagactgt gctgttcgat accggtacaa
     4441 agcctgccat cttcagttca aactttaagt tgacgggcat cgacgccggg tctattgaca
     4501 tcctggcatt ttcgcacgaa cacctcgatc atgtgggagg tctctctgca tttgctgaga
     4561 tgaagacagg gatcccggct gtcattcctc attcctttac ccctccggtt gtctcatcca
     4621 tcgtaaacgc cggctacata cctctcctgg tcagagatgc cgcaatgata tgcaacaacc
     4681 tgtatacatc aggggaattt gatttcggga ttgccgagca gtgtcttgtc cttgatacaa
     4741 gggcaggact cgtggtgatg acggggtgtg ctcatccggg aatagtgagg atgctcagtg
     4801 aaatcaaaca aacattcaac aagaacatag ttgccgtttt cggaggcttc catcttatgg
     4861 acaaatcaaa agccgggacg gaggagatta ttgccggtat gaaatctctc ggaattgtaa
     4921 gatgcggcgc cacccactgt acaggtgaaa agcagattga catgttccgc gaagcgtttg
     4981 gcgaaaacta ttttgagctc ggtgccggta acagggtaat cttcaactga acaacccctg
     5041 atctgctccc tgagggtttt ccgggggaac aatcagccat gtttcatgtt tatccgatat
     5101 gggcggaact gttttatatg acggtgagtg cagcttttgc aacagccagg tgagattcat
     5161 ccgggagcgc gacacgcgga acaggttccg tttcgttccg ctgcaatcag atgagggaag
     5221 ggagttgttg cgcaaagccg gattacctga aaatgaactt gattctgttg tgtatgacag
     5281 gtcagggcgg caatacctcc gctcatcagc cgttctgggc attttaagag atatgggccg
     5341 tggatggtcg ctgctttatt ctctggtgat tgtacctgcc tgcatccgtg atgtcttcta
     5401 ccggctcgtt gcaaggaatc gtcaccggct gactccacgg agcagatctt agcaagctga
     5461 tcattttccc tgtaattatc atgcctgtta aacaggaatc tgcttaaatt gcatcaaatt
     5521 atcaaagcca tgatactgag tacatgcgga ctcaaatgcg atgaatgcga gttctacaac
     5581 aaaacctgtt ccggctgccg taacgttatg ggagccacct tctgggcaat tgagatgatg
     5641 cccgcaaagg tatgcccgct ctatgactgc gcagtgaacc agcggggtca taaagattgc
     5701 ggcgagtgcg ccgaacttcc gtgcgcaact ttccttcaga tgaaagaccc tgccacctcc
     5761 gacgaggagc accagcgtat gataggcatc agggttgact tgctcagggc gacggccaac
     5821 tgaccaccac agcctccccg ttgaccatgg aggagatcac ccgcaaatcg ctgctttaca
     5881 ggagcgggct gggcttctgg tgcattaacc atgtccacgg ctgcagccac ggctgtaaat
     5941 atccgtgcta tgcctggatg atggcacact ctcacggcag ggtgggaacc tatgccgaat
     6001 ggtgcagacc ttcactggtc gttaacgccg aggctctgct ctcaaaagag ctggagcgaa
     6061 tgaagtcaaa gcccggcaac atccatctgt gcctgacgac cgatccgttt atgtatggtt
     6121 acccggaagt gacatccctg accctgcgca tgatcgaact gatcaactca tatgggatta
     6181 gttgttccac cctcaccaag ggtgtcacgc cggttgacct ggccgactcc atcaggttcc
     6241 cggagcagaa ttcgtacggt atcagcctgg tgtcgctcag cgaggagttc agaaaaaagt
     6301 gggagccggg agctgcaccg tacaatgaac gtatcggcgc attacgcgaa ctgcatgatc
     6361 ttggctgcag cacccttgtg catatcgaac cttaccccac accgaacatt attgaacagg
     6421 acctccggga actgcttgat gccgttgact ttgccgatga gatatggttt ggctcctgga
     6481 actataacaa cctcgccaaa cagtacccgg gcttcaggga attctatgac gagcagtcgg
     6541 ccatcctcag ccgtttcctc tccacccgcc aagactcgct atagaatcgc cgttggcggt
     6601 caggaattgt atgccaatta atcgccgtag gcaataaagc attgtatacg caaggcaccc
     6661 ccatccaccc gcaccgcact ttattctccg atggacaaaa agacaggaag accccatatt
     6721 cccccaccta tctgtaaatg acgtatcttt atgctggata atagtgaccg gatgaggagc
     6781 ctgcttcaaa aatacctgtc agcactggtg ctcatgtcag cagtttcgtc tgtggcttca
     6841 ggacaaccct ggtacgtggc caccaccggc tccgactcca acgccgggac actcagcagc
     6901 cccttcaaat ccattgaaaa agctgtctcg gtggtaaaag gcggggagac aatctatctc
     6961 cgcggcggca catataacct gacagcaaca ataaccctca gcaagagcgg cgcagagaac
     7021 acggtcattt cagtcatcgc atatcccggg gaggtgcctg tactcaattt ctcagcacag
     7081 acacaaagct ccggcaacag gggcctcgtg ctgacaggca gctggtggca cataaaaggg
     7141 atcagcatca ccggggcagg cgacaacggg atgatcatca gcggcggaaa caataacctc
     7201 atcgaccagt gcagcttcta tcgcaatcgc gacacggggt tgcagattga caacggcgca
     7261 tcagataacc aggttcgcaa ctgcgactcc tacttcaacg ccgatccgcc tgacaacgac
     7321 gatgccgacg gctttgcgcc gaagctcacc gccggcagcg ggaacagctt ctacggctgc
     7381 cgcgcatggc gcaacagtga tgacggctgg gacggatacc tcaggggaac caatgatatc
     7441 accaccaccc tcgaatattg ttgggctttt gagaacggat tcctggaaaa cggcaccgat
     7501 gccggcgaaa atgccaacgg caacggtttc aagataggag ggagcgatga taaaatgctg
     7561 atgcataact tcatcctgaa gaattgtctg gcctttggca acaagtcgaa ggggtttgat
     7621 cagaacaaca acaggggatc gatgactctc tataactgca ccggccataa taacggggca
     7681 gataattacc gcatcagtca ggcactggcg gccggcaagg cgcttaccct gaagaactgt
     7741 gccgaactcg ggggcaaggt gagcatcggc accttcgcgg tgcaggagaa aaacagctgg
     7801 ctgacgcc
//
LOCUS       DAOPXU010000120         7672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_11500_length_7672_cov_14.381384, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000120 DAOPXU010000000
VERSION     DAOPXU010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7672
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11500_length_7672_cov_14.381384"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PKK03_10435"
     CDS             complement(<1..292)
                     /locus_tag="PKK03_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84859.1"
                     /translation="MKKLTLFAFVMLLAGTTGEVWSCTTFLMSGKHTPDGRPLLYKNR
                     DTGTLDNALVYFTDGKYDYIGLVDSKESWKREVWGGFNSAGFAIMNSAAYNNN"
     gene            complement(532..2199)
                     /locus_tag="PKK03_10440"
     CDS             complement(532..2199)
                     /locus_tag="PKK03_10440"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNX84860.1"
                     /translation="MKKAGFLVTVILSVISYFITVPLAAQPLNVKKLAVDYDKMLLEQ
                     FKPDETGCAALVAKDGQVIYTRAFGMANLELNVPMQPDMVFRIGSITKQFTAVAILQL
                     MEQGKLSLQDDVTKFIPDYPTQAYTITIEHLLTHTSGIKSYTNLPGYIENVRTDMKPD
                     ELINTFKNQPMEFAPGTKWNYNNSGYFLLGYIIEKVTGKSYPDYIQENFFTPLGMTST
                     CYGSDTKIIKNRAYGYQQGGDGVANSDYCSMTQPYSAGSIMSTVGDLFKWHQALYAGK
                     LVKKETLEKAVTEYRLSNGKGSGYGYGWFLSQLQGSPTVEHGGGIFGYLTSSVYLPKE
                     DVYVALFSNSTAKAPEFTALKMAAMAIGKPLVTTGITLDEATLDQYAGVYFNDAGREV
                     TVTRDGNQLSATLTGAGTRKMFPVEKDKFLVDDAFMYATFSRDAAGKIVSFVSDDRGR
                     LDEWKLTDKKIEEKKAITLSEATLDRYTGEFELQPGFTITFTREGNRLMTQATGQGKF
                     EVFAESETKFFLKVVEAQVEFVADPDGKFNRMILYQGGQKVEGKRIK"
     gene            2312..2797
                     /locus_tag="PKK03_10445"
     CDS             2312..2797
                     /locus_tag="PKK03_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNX84861.1"
                     /translation="MKLFIAAIALLIFGLAPLPAQKIEKIGIPGLEKILASSEDQLYV
                     VNFWATWCPPCVTELPYFEKLSKEYQGKGVKFILVSLDFPSQIDSKLIPFLKKNKITA
                     DVRLMTNLDYNSWIDKVDPGWQGNLPATLFFNNAEKTKYFHPSEVDEKELRDLITKYK
                     Q"
     gene            2821..3429
                     /locus_tag="PKK03_10450"
     CDS             2821..3429
                     /locus_tag="PKK03_10450"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNX84862.1"
                     /translation="MKKYLFISVAALMMTGLLNAQSQYKAGDIASDFNLKNVKGEPVS
                     LTDIKDADGFIVAFWCNTCPVVKKYDQRLQELHAKYAPKGYPVVAINANDPAVSPGDS
                     YDRMKETAAKLNYKFEYLFDETQETARKYGATNTPHIYILSWKDGRLVVDYVGAIDNN
                     ADDATAADKKYAEDALNSILKGQAVAVAGTKAVGCSIKWKKS"
     gene            complement(3480..3707)
                     /locus_tag="PKK03_10455"
     CDS             complement(3480..3707)
                     /locus_tag="PKK03_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84863.1"
                     /translation="MAGPNNSFIKKQKTEKRLKKRKEKEQKKQLRRENSPGGGLDNMI
                     AYVDKFGNITSEPPAEDAAIKPGETETAPGR"
     gene            complement(3720..4925)
                     /locus_tag="PKK03_10460"
     CDS             complement(3720..4925)
                     /locus_tag="PKK03_10460"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNX84864.1"
                     /translation="MEYKKDRRSAKNRTYKKGKKETANFNSRKKHNAGELPPESFVRK
                     AVPADETIYRAGRLITDLPVEGSIISNLRKKGYEVPTEIQDKTLEALLEGRNLIGLAQ
                     TGTGKTAAFLIPLINNLIRKDPSGNVLVISPTRELALQTDTEFRSIAHGLKLFSTCLI
                     GGTSVRHDIEKLRKPGHVIIGTPGRLADMVRQGALDLRRFSVLVLDEFDRLLDMGFAP
                     EIQKLVEGMGRRKQTILFSATEDSSQRKIIDGLMENPFVVRVRTGNISADNIEQDIIS
                     VKDGERKIEILLEMVREDSFEKVLVFAETKRGVSRICRDLRRGGITADEIHGDKSQNY
                     RIKALESFRNRKIKVLVATDVAARGLDISHVSHVINFQQPRDMESYIHRIGRTGRAGA
                     SGRAITFVN"
     gene            5295..5855
                     /locus_tag="PKK03_10465"
     CDS             5295..5855
                     /locus_tag="PKK03_10465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNX84865.1"
                     /translation="MKKIAIISPSVRQGRKSHRVALWFRSYLKQNNLAETDIIDLSEY
                     RFPLFEERLKMQQTPLPEAIEIAGRVRSADGVIIIAPEYNGGYPASLKNVIDLLTDEW
                     RRKPVAISTVSDGPFGGTQVITSLQFTLWKIGTITVPAMFPVPRVEEAFSETGVPAEA
                     EAMNRRSARFTGELLWFIEAKARMES"
     gene            6004..7509
                     /locus_tag="PKK03_10470"
     CDS             6004..7509
                     /locus_tag="PKK03_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84866.1"
                     /translation="MKKFFCTTILTVLMASAAIAGRQDYPEEYLGLPGDNLNLYAVMN
                     LFQESETLEAFERSLNEENSRINNLDLNNDGYVDYLTVSDFVSGNVHTIVLRSVLDRN
                     EYQDVAVFTVEQLRNGEVQIQLIGDEALYGRNYIVEPNYAETPNPGYTGRTVQRNNVT
                     VVTTTYYEVAAWPVIRYIYSPRYVIWRSNWYWGYYPVYWHPWRPFFWHHYYGYHYHWY
                     PHYYAHYRHWHQPRWNHYHDYYYGSVRAYSPNVSHRITAGSYKQTYSRPETRRDGEAL
                     YASTAGTRSRSVGSQATAGRRQSETAAAAQDQSRTRSVGTTAGRRQSETAAAAQGSET
                     RRSATTQPATRTQSSSSGRNSAPATRTQSSTSRSSSAPAARTQSSTSRSSSAPAARTQ
                     SSTSGRSSAPAARSQSSSSGRSSAPAARTQSSSRSSSVPAARTQSSSRSRSSSTPAAR
                     TQSNSSGRSSGTVSSRSSKSSSRSSGTPSKSSSRSSSSSGKSDNSSRSSRR"
BASE COUNT         1883 a         2012 c         1908 g         1869 t
ORIGIN      
        1 tgttgttgtt ataggcagct gagttcatga tggcgaagcc tgcggagttg aatccgcccc
       61 atacctcgcg cttccacgac tccttcgaat caaccagccc gatatagtcg tacttgccgt
      121 cggtgaaata tacaagggcg ttgtcgaggg tgccggtatc cctgtttttg tacagcagag
      181 gcctgccgtc aggggtgtgc ttgcctgaca tgaggaatgt ggtgcatgac cagacctcgc
      241 ccgtagtgcc ggcaagcagc atcacgaacg caaacagtgt cagtttcttc ataggggtta
      301 aaatatagtt tggataaagt tatgtttttt ttaaccccgt caggtccgcc gggtaacatg
      361 ttaagatttt ttaacccgca accccgtccg tggcattgaa aaccacaatt tctgaccggt
      421 actgccttcc cgactgctac tgccgaccgc cgactggcac tgccttcccg atccgccggc
      481 cggcggatgc gggactgatc ctgcgctgct gctgcagtct cagactgcca actattttat
      541 cctcttccct tcgaccttct gccctccctg gtaaagtatc atcctgttga atttcccgtc
      601 agggtcagcc acaaactcaa cctgggcttc gaccaccttc aggaagaact tcgtctcgct
      661 ttcagcgaaa acttcgaatt taccctgtcc tgttgcctgt gtcatcagcc ggttgccttc
      721 acgggtgaac gtgattgtga atccgggctg cagttcaaat tcacctgtgt acctgtcaag
      781 agtagcttca ctgagtgtga ttgctttctt ttcctctatc ttcttgtccg tcagtttcca
      841 ttcatcaagc ctgcccctgt cgtcagacac gaatgatacg attttaccgg cagcatcacg
      901 gctgaaggtt gcatacatga atgcatcatc aaccaggaac ttatcctttt caaccgggaa
      961 catcttccgc gttcctgccc ctgtcagggt ggccgagagc tggttcccgt ccctcgttac
     1021 tgttacctcc ctgccggcgt cattgaaata aaccccggcg tattgatcaa gggttgcttc
     1081 gtcaagcgtt atcccggtgg ttaccagcgg cttgcctata gccatggcgg ccatcttcag
     1141 agctgtgaat tcaggtgctt tggcagtgct gttggagaag agtgcaacat agacatcctc
     1201 tttcggaagg tagactgagc ttgtcaggta accgaaaatt ccgcctccgt gctcaactgt
     1261 ggggctgccc tggagctggg acaggaacca gccgtagccg tacccggaac cttttccgtt
     1321 gctaagcctg tactcagtaa cggctttttc aagagtctcc ttttttacca gtttgcctgc
     1381 gtagagcgcc tggtgccatt tgaagagatc accgacggtt gacattatgg aacctgccga
     1441 gtaaggttgt gtcatgctgc agtagtcgga attcgccacg ccatcgcccc cctgctgata
     1501 accataggcg cggtttttaa taatttttgt atcacttccg tagcatgtgg aggtcattcc
     1561 gagaggggtg aaaaaattct cctggatata atccgggtag ctctttccgg tgactttctc
     1621 aatgatatac ccaagcagga aataacctga gttgttataa ttccatttcg ttccaggggc
     1681 aaattccatc ggctggtttt tgaaagtatt tatcagttcg tcgggcttca tgtcagtcct
     1741 gacattctca atgtaaccgg gcaggtttgt atagcttttt atccctgagg tgtgggtcag
     1801 cagatgttca atagtaatag tataggcctg tgtcgggtaa tcagggataa attttgttac
     1861 atcgtcctgc agtgagagtt ttccctgttc cattagctgg aggattgcaa ctgcagtgaa
     1921 ttgtttggtg attgagccaa tcctgaatac catatctggt tgcatcggca cattcagctc
     1981 gaggttggcc atgccaaagg cccgggtgta tatcacctga ccgtcctttg caaccagagc
     2041 tgcgcagccg gtctcgtcag gcttgaattg ttcaagaagc attttgtcat agtccacagc
     2101 aagttttttg acattaagcg gctgtgctgc aagaggaaca gtgataaaat aagagatcac
     2161 tgacagtata actgtcacaa ggaatcctgc ttttttcatg gtcatggttt tatgatggta
     2221 ctcaataaag acgacatttg ggcatcagtg ctgctgtgaa catctgaggg ctgcagttgt
     2281 ttcattaaag ttatgcaaaa tgagtaactg tatgaaatta tttattgcag caattgcatt
     2341 gctcattttc ggactggcgc cattaccggc acaaaagatc gaaaagatcg gtatccccgg
     2401 actggaaaag attcttgcca gctctgaaga tcagttgtat gtggtcaact tctgggctac
     2461 ctggtgcccg ccatgtgtca cagaactgcc atactttgaa aagctttcca aagagtacca
     2521 gggcaaggga gtaaaattca tcctcgtaag ccttgatttc cccagccaga ttgactcgaa
     2581 actcatcccc ttcctgaaga aaaacaagat caccgctgat gtaaggctga tgacaaacct
     2641 cgactacaac agctggatcg acaaggttga tcccggctgg cagggaaacc tcccggcaac
     2701 cctgttcttc aataacgctg agaaaacaaa atactttcac ccctcagagg tggatgaaaa
     2761 agagctgaga gatctgataa ccaaatacaa acagtaatta ttaataaaac cgacaagatc
     2821 atgaagaagt acctgtttat atctgtggcg gcccttatga tgacgggcct cctgaacgcg
     2881 cagtcgcaat acaaggctgg cgacatcgca tctgatttca atcttaaaaa cgttaaaggc
     2941 gaaccggtat cactgacaga tattaaagac gctgacggat tcatcgttgc cttctggtgc
     3001 aacacctgcc cggtggtgaa gaagtatgac cagcgcctgc aggagctgca cgcgaagtat
     3061 gcacccaagg gatacccggt ggttgcaatc aacgcgaatg acccggccgt ttctccgggc
     3121 gattcatatg acaggatgaa ggagaccgcc gctaagctga actataagtt tgaatacctc
     3181 tttgatgaga cccaggagac agcacgtaaa tacggagcaa ccaatacacc acatatatat
     3241 atattgtcat ggaaggacgg cagactggtt gttgattacg tcggtgccat cgacaacaat
     3301 gctgatgatg caaccgcagc agacaagaaa tatgcagagg atgccctgaa cagcatcctg
     3361 aagggtcagg ccgtcgccgt ggccggcacg aaggctgtgg gatgttccat caagtggaag
     3421 aaaagctgag ttgccccgat aaggtaaacc tgtttaactt aatgaatggc atccggcatt
     3481 tacctgccgg gtgccgtttc tgtttccccc ggtttgatag cggcatcttc agccggcggc
     3541 tcggaggtga tgtttccgaa cttgtcgacg taagctatca tattatccag tccgccaccc
     3601 ggtgagttct ccctccgaag ctgcttcttc tgctcctttt cctttctctt cttcagacgc
     3661 ttctcggtct tttgcttttt aatgaaactg ttgtttggtc ctgccatata ttactgattt
     3721 taattaacaa atgtgattgc cctgccgctt gccccggctc tgcccgtgcg gccgatacgg
     3781 tgaatgtagc tttccatgtc tctgggttgc tggaagttaa ttacatgtga tacgtgagag
     3841 atatcaagcc ctctcgcagc cacgtcagtt gccacaagca cctttatttt cctgttcctg
     3901 aaagattcaa gggctttaat cctgtaattc tgcgatttgt cgccgtgaat ctcatcggct
     3961 gttatgccgc ctctgcggag gtctcggcat atcctgctca ctccacgctt tgtttcggca
     4021 aagacgagca ccttctcaaa agagtcttca cgcaccattt caaggagaat ctcaattttc
     4081 ctttctccat ccttaactga gatgatatcc tgttctatat tgtcagcgct gatgttgccg
     4141 gtacggaccc tgacgacaaa cgggttttcc atcagtccgt caataatctt tcgctggctg
     4201 ctgtcctccg tggctgagaa aagtatcgtc tgtttccggc ggcccatccc ttcaaccagt
     4261 ttctggatct caggggcaaa gcccatgtcg agcaaccggt caaactcatc cagcacaagt
     4321 accgaaaacc tccgcaggtc gagggctccc tgccgcacca tgtcggccag ccgacccggc
     4381 gtgccaatga ttacatggcc cggttttctc agtttttcga tatcgtgcct gacgcttgtg
     4441 ccgccaatga ggcaggtgct gaagagcttc aggccatggg caatgcttct gaactcggtg
     4501 tcggtctgaa gtgcaagctc acgtgtcgga gagatcacca gaacattgcc tgacgggtct
     4561 tttcttatca gattattaat taaaggtata agaaatgctg cggtctttcc tgtccctgtc
     4621 tgagccaggc ctataaggtt ccggccctcc agcaatgctt ccagggtctt gtcctgaatc
     4681 tcagtgggca cctcgtagcc cttctttctc aggttgctga tgattgaacc ttcaacgggg
     4741 aggtcagtga ttaatcttcc ggccctgtag atagtttcat ctgcaggaac agccttcctg
     4801 acgaagctct cgggagggag ctcccctgcg ttatgttttt tcctgctgtt gaagtttgcc
     4861 gtttcttttt ttcccttttt gtaagtcctg ttttttgcag atcgtcgatc ttttttgtat
     4921 tccattctga attaattggt taatgcctct gaaaaatcat taaccagata tctgttcaaa
     4981 atggtaatgt tcttatggcg aaaaaatgac tgagcctagg ctggtggtgg tagaaaggat
     5041 ctctgaaaaa tttcggggag attcgcggtc tatacagatc taacctatgc cagaatcgcg
     5101 gcaaagatac atttttttca gataccccgc aaacaggtca tggctgaatg ccggtcggtg
     5161 tcaatccgcg gacagtccac gagacattgc cggcgaaagc ctgcctgagt acaggcgatg
     5221 gatcaaagcc ggaggaaacc gcctcgcgga gggcaacccg ggcagcgttg aaaataagtg
     5281 ttatcttggc ggatatgaaa aagatcgcta tcatcagccc aagcgtgagg cagggaagga
     5341 agagccacag ggtggcactc tggttccgta gttacctgaa acagaataac ctggcagaaa
     5401 ctgatatcat tgacctttcg gaataccggt tcccgctctt tgaagagagg ctgaagatgc
     5461 agcaaacacc gctccctgaa gcgattgaga ttgcagggag ggtcaggtcg gctgatggcg
     5521 taatcataat cgcaccggaa tacaacggag gttacccggc cagcctgaag aatgtcatcg
     5581 acctgctcac cgatgagtgg cgccgcaagc ccgtagccat ctccactgtt tcagacggcc
     5641 ccttcggggg aacacaggtc atcacctcgc tccagttcac cctctggaag atcggaacga
     5701 tcacagtccc ggcaatgttc cccgtgccac gggtcgaaga agcattcagt gagacaggtg
     5761 taccggcaga ggccgaagcc atgaacaggc gctctgccag gttcactggc gaactcctgt
     5821 ggttcatcga ggcaaaggca aggatggaga gctgacgctt tctctcctgc cccatggctg
     5881 cccctgatcc atgtcttttt atatttttca aataatgagc cacatagcca ttttgtgcaa
     5941 tctttggcat aacttttgaa ttttactatc gcgaagagac agttcttcgt aaaacgtaaa
     6001 gagatgaaaa agtttttctg cactacgatc ctcacagtcc tgatggcttc agcggcaata
     6061 gccggaaggc aggattatcc tgaggagtac ctgggtctcc cgggtgacaa cctgaacctc
     6121 tacgcagtca tgaatctttt ccaggagtca gagactcttg aggcctttga aagaagcctt
     6181 aacgaggaga actccaggat caacaacctc gacctgaaca acgacggtta tgtcgattac
     6241 cttaccgttt ccgattttgt cagcggcaat gtccacacga tagtcctccg ttcggttctt
     6301 gaccgtaacg agtaccagga cgtggcagta ttcaccgttg aacagcttcg caacggcgag
     6361 gttcagatac agctgatcgg cgacgaggct ctttacggaa ggaattacat agttgagccc
     6421 aactatgctg agacgcccaa tcccggttac accggacgga ccgtccagag gaacaatgtg
     6481 accgtggtca ccaccaccta ctatgaggta gcagcctggc cggtgatcag gtacatctac
     6541 agtccccgct acgtcatatg gcgctccaac tggtactggg gttactaccc ggtctactgg
     6601 catccctgga ggccgttctt ctggcaccac tactacggtt accactacca ctggtatcca
     6661 cactactacg ctcactaccg tcactggcac cagccgaggt ggaaccacta ccacgactac
     6721 tactacggca gcgtaagagc ttactcaccc aatgtatcgc accgcatcac tgccggttca
     6781 tacaagcaga cctactcgag gcctgaaaca aggagagacg gtgaagctct ttacgccagc
     6841 acggcaggaa cacgcagcag gtcagtcggc agtcaggcaa cagccggaag gaggcagtct
     6901 gagactgcag cggcagcgca ggatcagtcc cgcacacgca gtgtcgggac aacagccgga
     6961 aggaggcagt ctgagactgc agcggcagcg cagggcagtg agacacgcag gtcggcaacg
     7021 acccaacccg caaccagaac tcagagcagc agcagcggac gcaacagcgc acccgcaacc
     7081 agaactcaga gcagcaccag cagaagcagc agtgctcccg cagcaagaac tcagagcagc
     7141 accagcagaa gcagcagtgc tcccgcagca agaactcaga gcagcaccag cggacgcagc
     7201 agtgcaccgg cagcaaggag ccagagcagc agcagcgggc gcagcagcgc accggcagcc
     7261 aggacccaga gcagcagcag gagcagcagc gtaccggcag ccaggactca gagcagcagc
     7321 aggagcagga gcagcagcac accggcagca aggacccaga gcaacagcag cggacgcagc
     7381 agcggaactg tctcaagccg cagcagtaaa agcagcagca gaagcagcgg caccccttca
     7441 aagagcagca gcagaagcag cagctcatca ggaaaatcag acaacagcag ccgctcatcc
     7501 cgaagataag gggcacagag caggttgctc agtgcattga ttataccgcc aggtgcaggg
     7561 gaaaaacacc cctgcacctt tttttttttc atgtacctga acccctattg tgaattagaa
     7621 tattttctat ttacttagat gcgcttaact taaacctaaa aaccaaacta gt
//
LOCUS       DAOPXU010000121         7481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_11939_length_7481_cov_10.133585, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000121 DAOPXU010000000
VERSION     DAOPXU010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11939_length_7481_cov_10.133585"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1214)
                     /locus_tag="PKK03_10475"
     CDS             complement(63..1214)
                     /locus_tag="PKK03_10475"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX84867.1"
                     /translation="MTSFLTDTSVKMIYSVMPMFLMSIGASKASLGLIEGVAESTASL
                     IKALSGFWSDRLGRNKPFMLVGYAISAIVLPLYAFVISPMQVLILRFAERFGKGIRTA
                     PRDSLIAASVTNNETGKGFGLQKAMDNSGAIAGPLIAFAVLSLFPDNYRLIFLIAGIP
                     AIAGIFVIIFGIKEVKKNKTELLQKFRFRDYPKRYWFFLAIIFVFTLGNSTDALLLVK
                     ANEAGIKVAFIPLVYLITNLVSVVAAIPAGSLSDRIGKERLLVAGFLIYAVVYLFFGM
                     NVGEGALFVLFALYGLYSAATDGIQKAFVADVADANKKGTALGIYNAMLGITLLPASL
                     IAGILYDRVNSSVPFIFGAAMALLAAILMAVYMFTGRKHPATDSRQQCL"
     gene            complement(1334..4009)
                     /locus_tag="PKK03_10480"
     CDS             complement(1334..4009)
                     /locus_tag="PKK03_10480"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HNX84868.1"
                     /translation="MTEAYRQEAADLIRELGANESSGLTTAEVKRRQEQYGFNQLESK
                     SKKSFLRMFLEQFRSFMIIVLLIAAAISGVVGVMEGEGLLDTYIILGILLVNALIGAV
                     QEKKAETSLEALKELASPLSKVLRDGAISEVNTRELVPGDIVILETGAVIPADIRLTE
                     AINLKIQESSLTGESVPVDKVTNALTGDDIPLGDRKNMAFSSGMVTYGRGRGVVAATG
                     MHTEVGKIADMLQHAGETETPMSKRLNQLGRLLGIVALSICAVIFVVGVLYGNSVISM
                     FMTAVSLAVAAIPEGLPAVSTIVLAMGVQRMVKRNAIIRTLPSVETLGSATVICSDKT
                     GTLTQNRMTVVEAYVNHKHDLINRSAPGSVLNDEENRLLSISVLCADATMKVNQDGKF
                     TFTGDPTETALLDFGVLYNVYKDELEKSFPRVAEIPFDSERKRMTTVNRMGEKETRVN
                     VKGGLDEVLNVCDKIVIKGEVRSIEKDDIEKIREANESMANSALRVLAMAYKDYHEAP
                     QQVTIGQIENGLIFIGLLGMIDPARPEVVDAVARCNTAGIRPVMITGDHKSTALAIAR
                     EIGIYREGDVAITGTELEKLSDEELEKSVCRFSVYARVAPEHKVRIVKAWQSHSEVVA
                     MTGDGVNDAPALKQADIGAAMGIVGTDVAKGAADMILTDDNFATIVSAVEEGRRIYDN
                     ILKVIQYLLSTNVGEIFLILVTSVFNMGMPLLPIHILWINLVTDSLPALALSVDPPEK
                     GIMNRKPRNTMKGFMTRGMVWRVLYQGLMIGCIPLVSYILGKHDGGEVLGRTMAFATL
                     MFAQLVHVRNLHSNEKSSFLFNPLRNKALIGAIFVSAMIGLIVLLVPPVREAFSLAVM
                     DREHWIIVILMALAPIPVVELFKMFRINGVKPDKD"
     gene            complement(4189..4659)
                     /locus_tag="PKK03_10485"
     CDS             complement(4189..4659)
                     /locus_tag="PKK03_10485"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HNX84869.1"
                     /translation="MVDEAPEKRFILDVHLGRLAKYLRLCGFDSLFSAFFNDREIIEE
                     AVRESRVILTRDRLLLKNATAATGYLVKSQYHEEQLREVFEALRLREHVRLFSRCITC
                     NAVLEEVKKEEIADRLLADTKKYFSVFRRCPGCDRIYWEGSHYDNMKKSLASLL"
     gene            complement(4754..7123)
                     /locus_tag="PKK03_10490"
     CDS             complement(4754..7123)
                     /locus_tag="PKK03_10490"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX84870.1"
                     /translation="MIRNLIKTAVRHILKHPGYSFLNVLGLTLGITSALFLLIYVSDE
                     VSYDRYHEKADRIYRVSSRITETDDQFTWIVAQIPFGPQVAQDYPEVEAYVRFFNMPR
                     ALYEYEDKEFVEEDFFYADSALFDIFTYRVIRGDARRALLEPNKIVLTETVAARYFGD
                     GDPVGRTLTSGNSAYEVTGVIEDVPSNSHFRFGALASRTNLPEQIGSWGNFGVFTYLL
                     FPEGLDVKAFEEKMQGMYAAYMEPIFGPVNVKVEYLLEPIREIHLYSTKEGEPEPTGS
                     ITYVYIFAIVAIFLVLIAAMNYMNLSTARSSGRAREVGLRKVVGSRRGPLVLQFLSES
                     VIFTVLSLAISIILLMALLPKLNLLAGKSFDSNVIFSPVVLLGMLGVVLLVGFIGGSY
                     PAFFLSRFSPVTVLKGELTHGTAGSLFRKILVVIQFAVSVIMIVCTLVVFRQLNYLKN
                     MDQGFDQKNVLSLDLNGPMAMKYPVLKQALLENPNVLYVSSTSTRLGEGSGKLLFDVE
                     TDQGMVQRGINFAIADHDFVEALGIKMVQGRDFQKDMPSDTLTGVIVNETFVRRMGWD
                     EPIGKKVEAGDENTLRARVVGVMKDYHQTGMYNEIESLLLAYRTLNNIVYIKVSDENV
                     EQALSHIESSWKEVFPDQPYSYTFLSERFNRQFESDEKRGLIFTLFTVLAILIACLGL
                     FGLASYTVEQRTREIGIRKVFGANEGTILKLVTRDFIILSLISIVIAFPVAWYFMSKW
                     LENYVYKSSMGPVVFILAGVLTLVITFATISYKAYQASVSNPAEAISTV"
BASE COUNT         1901 a         2011 c         1673 g         1896 t
ORIGIN      
        1 ggtctgaggg tctgagggtc tgagggtctg agggtctggc aacctatatt attgattatc
       61 tgctataaac attgttgtct gctgtctgtt gccgggtgct ttctgcctgt aaacatgtag
      121 actgccatca ggatggcggc gagtaacgcc atggcagcgc caaatatgaa agggacggat
      181 gaattgaccc tgtcatacag tatgcccgca ataaggcttg caggaagaag ggttatcccg
      241 agcatggcat tgtaaatgcc aagggcagtc cctttcttgt tggcgtctgc cacatcagcc
      301 acaaaggcct tctgtatgcc gtcagtagct gccgaatata acccgtaaag tgcaaagagc
      361 acaaaaagag cgccctcacc cacattcatg ccgaaaaaga gatagaccac cgcgtaaatc
      421 aggaaccccg ctaccagcag cctctctttc ccgatcctgt cggatagcga tccggcgggt
      481 atggcagcaa caactgacac gagattggtt ataagataaa ccaaaggtat gaaagcgacc
      541 tttatcccag cctcattggc tttgacgagc agcagggcat cagtcgagtt tccgagagta
      601 aaaacaaaga tgatggcaag aaagaaccag taccgtttcg gataatccct gaagcggaac
      661 ttctgcagaa gctcagtctt attctttttc acctccttta tcccgaatat tatgacaaaa
      721 atcccggcaa tggcgggtat cccggcaatc aggaaaatga gccggtagtt gtccgggaac
      781 aggctgagga cagcaaatgc tatgagtggt cctgcgatgg cgccgctgtt gtccatcgcc
      841 ttctgcaaac cgaatccttt gcctgtctca ttattggtga cagacgcagc aataaggctg
      901 tcacggggag ccgtgcgaat gcccttgcca aatctctcgg caaacctgag aatcagaacc
      961 tgcatcggtg atatgacaaa ggcatacagc ggaagaacga ttgcagagat ggcgtaaccg
     1021 accagcatga agggcttgtt cctgcctaac ctgtcactcc agaaaccaga cagcgccttg
     1081 attagagaag ccgtgctctc ggcgactcct tcaatcagtc caagacttgc cttggaagcg
     1141 ccaatggaca ttagaaacat cggcatgacc gaataaatca tcttgacgga tgtgtccgta
     1201 agaaaactgg tcatgcctgt aaaaagaata tttctttcga acccgaatat cctggattcg
     1261 tgcaacaccc ttccggaccc ggaatccggt ttgccctcgc ctgtcatgtc atcttaagtc
     1321 cgcacctgcg gctttaatct ttgtctggct tcacaccgtt gattctgaac attttgaaca
     1381 actcaacaac gggtattggt gccagcgcca taaggattac aattatccag tgctcacggt
     1441 ccattaccgc caggctgaag gcctcacgta ccggcggcac gagcagaact atcagcccga
     1501 tcatggcaga tacaaatatt gccccgatca acgccttgtt tcttaacgga ttgaacagga
     1561 atgacgactt ctcattggag tgcaggttac gtacatgcac cagctgtgcg aacatcagcg
     1621 ttgcaaatgc catggtgcgg cccaggacct caccaccgtc atgcttgccg aggatatagg
     1681 aaaccagcgg aatacaacct atcatcagtc cctggtagag cacacgccac accatccctc
     1741 tggtcatgaa tcccttcatg gtgttgcgcg gcttgcggtt cattatcccc ttctcaggcg
     1801 gatcgacact cagggcaagc gccggcaggc tgtcggtaac aaggtttatc cacagaatat
     1861 gtatcggcag cagcggcatc cccatattga ataccgaagt gaccaggatg aggaaaatct
     1921 ctccgacatt tgttgagaga aggtactgga tgaccttgag aatattgtca tatatccttc
     1981 gtccctcctc aacggcagag acgattgtgg caaagttgtc atcggtgagt atcatgtctg
     2041 ctgccccttt ggcgacatca gttccgacta tacccatggc agcgccaata tctgcctgct
     2101 ttagcgccgg tgcgtcattc acgccgtcgc cggtcatcgc caccacctca ctgtgactct
     2161 gccatgcctt gactatccgc accttgtgtt ccggggccac cctcgcatag actgagaaac
     2221 ggcacaccga tttttccagc tcctcatcac tgagtttctc cagctctgtg ccggtgattg
     2281 ccacatcacc ctcgcggtaa atgccaatct cacgtgcaat ggcaagcgca gtgctcttat
     2341 ggtcacccgt gatcatcacc ggacggatac cggctgtatt gcacctggca acagcatcaa
     2401 ccacctccgg tctcgccggg tcaatcatac ccagcaatcc aatgaagatc agtccgttct
     2461 ctatctgacc tattgtcacc tgctgcggcg cctcgtgata atccttatag gccattgcaa
     2521 gtaccctgag cgctgaattg gccatacttt cattggcttc gcggatcttc tcaatatcat
     2581 ccttctcaat ggacctgact tctcccttaa tgacgatctt gtcacaaacg ttcagcacct
     2641 cgtcaagacc tcccttgaca ttcaccctcg tctccttctc tcccatcctg ttgacggtgg
     2701 tcatcctctt ccgctcggag tcaaacggaa tctcagccac ccttggaaat gacttctcaa
     2761 gctcatcctt gtaaacattg tagagcaccc cgaaatcaag cagggctgtc tccgtgggat
     2821 caccggtgaa ggtaaacttt ccgtcctggt tgactttcat cgtagcgtca gcacagagga
     2881 ctgatattga aagcagtctg ttctcttcat cattcagtac gcttccggga gcactcctgt
     2941 taatcagatc atgcttgtga ttgacatatg cttctacaac cgtcatcctg ttctgtgtca
     3001 gcgtcccggt cttgtcagaa cagattacgg ttgcactgcc cagtgtctca acggaaggaa
     3061 gcgttcttat tatggcatta cgctttacca tccgctgaac acccattgca aggacaatgg
     3121 tcgacactgc cggcagccct tcggggatgg ccgcaacagc caggctcact gcagtcatga
     3181 acatgctgat caccgaatta ccatagagaa cacctaccac aaatatgaca gcgcatattg
     3241 aaagggctac gattccaagc agtcgtccaa gctggttaag ccgtttactc ataggagttt
     3301 cagtctcacc ggcatgctga agcatgtcag ctatcttgcc aacctccgta tgcatgcccg
     3361 tggctgcaac aactcccctg ccgcggccat aggtgaccat gccgctcgaa aaggccatat
     3421 tcttcctgtc acccagaggg atatcatctc cggtcaatgc attggtgacc ttgtcaacag
     3481 ggacactctc gccggtgagc gatgattcct gtatcttgag gtttatggcc tccgtcagcc
     3541 tgatgtcagc cgggatcact gccccggttt caaggatgac aatgtcaccc ggcaccagtt
     3601 ccctggtgtt cacctccgag atggcaccat cgcgaagcac cttcgacaga ggcgatgcaa
     3661 gctccttcaa agcctccagc gaggtttcag ccttcttctc ctggacagcc ccgataagtg
     3721 cattgacaag cagaatgcca agtatgatat atgtatcgag aagtccctct ccttccatta
     3781 ctccgacaac tcctgagatg gccgctgcga taaggaggac aattatcatg aagctcctga
     3841 actgctccag aaacatccga agaaaactct ttttcgattt tgactccagc tggttgaagc
     3901 cgtactgctc ctgcctcctc tttacttctg ccgtggtcag ccctgatgat tcgttggcgc
     3961 cgagttccct gataaggtca gcggcttctt gtctgtacgc ttctgtcatt tgtcttttca
     4021 gataatttct gttgttgaat ccctcctacg accggcatca tccgccgccc tcacagcaga
     4081 tattgtccta aacgtcggtc cgggcattgg ccggactaaa tcaaggcacc aaaaatatta
     4141 attttaactg attgtaccta aatattatgc ctgaaccggc actacggttt aaagcagtga
     4201 tgcaaggctc ttcttcatgt tgtcataatg cgagccttcc cagtaaatcc tgtcacagcc
     4261 gggacaccgc ctgaaaacgg agaaatattt ttttgtgtca gcaagaagcc tgtcggctat
     4321 ttcctccttc tttacttctt ccagcactgc gttgcaggtt atgcaccttg aaaagagcct
     4381 gacatgctct ctgagcctga gcgcttcgaa tacctcccgg agctgctctt cgtggtattg
     4441 cgacttcaca aggtaccctg ttgcagcagt tgcattcttc agcagcaggc ggtcacgggt
     4501 gagtatcacc cggctctccc ttacagcctc ttcaattatc tcccggtcat taaaaaaggc
     4561 actgaagaga gaatcaaagc cacacagcct tagatatttt gccagccttc caaggtgtac
     4621 atcaaggatg aatctttttt caggagcctc atcaaccata gtgatataat ataatctccg
     4681 gcttaattgc cctgtcttac tccggcacca acaggaaaat tttccaattg cattatatgt
     4741 tacgagatta tttctataca gtactaattg cttcggccgg atttgatact gatgcctggt
     4801 atgccttgta gctgattgtg gcaaaggtta tcacaagagt cagcacgcct gccaggatga
     4861 ataccaccgg acccatactg cttttataga cataattctc cagccatttg ctcatgaaat
     4921 accaggcaac gggaaaagca atgactattg agatcagcga caggatgatg aaatcccttg
     4981 tcacaagctt caatatcgtg ccttcatttg caccgaagac ctttcggatg cctatctccc
     5041 tggttcgctg ttccacggtg tatgaagcca ggccaaagag gccaagacag gcaatcagga
     5101 ttgccagtac cgtgaacagg gtaaagatca ggcctcgctt ttcatcagac tcaaactgcc
     5161 tgttgaaccg ctctgagagg aacgtgtaac tgtatggctg gtccgggaaa acctccttcc
     5221 agctcgattc aatatggctg agagcctgtt cgacattctc atcactgacc tttatataga
     5281 ctatgttgtt aagagtccgg taagccagaa gcagagattc aatctcattg tacattcctg
     5341 tctgatgata atccttcatt acacccacca ccctggctct cagcgtgttc tcgtcgccag
     5401 cttcaacctt cttgcctatc ggttcatccc atcccattcg cctgacgaat gtttcattca
     5461 ctatgacccc ggtcagtgta tcagaaggca tatccttctg aaaatcccga ccctgtacca
     5521 tcttaattcc caatgcttca acaaaatcat gatcggcaat tgcaaagtta attccacgct
     5581 gaaccatgcc ctggtcggtt tcgacgtcaa agaggagctt tcctgagccc tctcccagcc
     5641 gggtggatgt ggaggacaca tacaatacgt tgggattttc cagcagggcc tgtttcagga
     5701 cagggtattt catggccatc ggtccgttca ggtcaaggct gagaacattt ttctgatcaa
     5761 acccctggtc catgttcttc aggtaattga gttgcctgaa aacaaccaga gtacatacta
     5821 tcattattac agaaactgca aactgaataa caaccagtat tttacgaaac aaacttcctg
     5881 ctgtgccgtg ggtaagctct cccttgagca cggtaaccgg gctgaacctt gagaggaaga
     5941 atgcagggta gcttccgccg ataaatccga caagcaatac tactccgagc atgcccaaca
     6001 gtaccacggg gctgaatatt acattgctgt caaaggattt tccggccagg agattcaatt
     6061 tgggaaggag cgccataagc aggataatgc ttattgcgag tgacaaaact gtgaatatga
     6121 ccgattcgga aagaaactgc aaaaccagcg ggccccggcg tgatccgaca acttttctca
     6181 agccgacctc acgtgcccgg ccggatgaac gggcagttga aaggttcatg tagttcattg
     6241 cggcaatcag tacaaggaag attgccacaa ttgcaaatat atagacatat gtgatgcttc
     6301 ctgtgggttc cggctctccc tccttggtcg agtacagatg aatctccctg atcggctcga
     6361 gaaggtattc aaccttgaca ttcaccggcc cgaagattgg ttccatatac gcagcataca
     6421 tcccctgcat cttttcttca aaagccttga catcaagccc ttccgggaaa agaagatatg
     6481 taaacacccc gaagttgccc catgaaccaa tctgctccgg cagatttgtt cttgatgcga
     6541 gggctccaaa cctgaagtgg gaattggaag gaacatcttc tatcacccct gtgacctcgt
     6601 atgcagagtt accggaagta agcgtcctgc cgaccggatc accgtccccg aaatacctcg
     6661 cggcaactgt ttccgtaagg acaatcttgt tcggttccag gagcgctctc cgggcgtctc
     6721 cgcgaatcac cctgtacgta aaaatatcaa acagggctga gtcagcatag aaaaagtcct
     6781 cctcaacaaa ctccttgtct tcgtattcat aaagtgcacg aggcatattg aagaacctga
     6841 catatgcctc cacctcgggg taatcctgcg ctacctgggg cccgaagggt atctgggcaa
     6901 caatccatgt gaactgatca tcagtctccg ttatcctcga tgaaacacgg tatatcctgt
     6961 cggccttctc gtgataacgg tcataactca cctcatccga gacatatatc agaagaaaaa
     7021 gcgcgcttgt aatgccgagc gtcagcccga gaacattaag aaaactatag ccggggtgtt
     7081 tcagaatgtg cctgacagct gtcttgataa ggttcctgat catacttttc tgttttagcc
     7141 ataaaaataa acctaatgga ataaccggat gctttagaat ttcaatttat tacttgatta
     7201 acagaatatt aaccctgcca aaaccggtga attgtatgat tcttttacac tctccgtatg
     7261 attattttac actttccaca atagcagcaa ttacatctta ttgattatat gcctattacg
     7321 agtgcggcac actctttgat taaaaacatc gaaaatttgg actgagatga agtatctgat
     7381 atgcatggct ttggttatgg cagctttttt tccccgggaa ctgagtgccc aggaaacaaa
     7441 atggactctg agtgactgca taacctatgc acttgagaat a
//
LOCUS       DAOPXU010000122         7323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_12323_length_7323_cov_12.819895, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000122 DAOPXU010000000
VERSION     DAOPXU010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12323_length_7323_cov_12.819895"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="PKK03_10495"
     CDS             complement(<1..951)
                     /locus_tag="PKK03_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNX84871.1"
                     /translation="MKSSDDLILSIDFGTQSVRSVLIDLEGNVLQLARVEIEPYFSVA
                     PGWAEQYPEYFRDKMFESLRKLFSETSYNKDRIKAVTLTSQRSTLINLDSEGKSLRPA
                     ITWLDQRLSAVGKYPGKMMDLALRLVGMREAVHFAVKNGECNWIMQNEPEIWKKTGKY
                     LLLSGYLTWALTGEYRDSVGCTVAYLPFDYKTQKWTDRKHMNYKMFPIDSEKLPDLVK
                     PSEVLGTISRGASELSGIPAGLPLIAAAADKASEVLGSGVINPDVACLSYGTTATVQT
                     THSRYHEVIPFFPSYPSAIPDCFNTEVQIYRGYWMISWFKK"
     gene            complement(961..2589)
                     /locus_tag="PKK03_10500"
     CDS             complement(961..2589)
                     /locus_tag="PKK03_10500"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNX84872.1"
                     /translation="MENGNSYRHILKWGDKHEEGISHHMAEVIKEKFDLSDADFKGKH
                     LPGTEPVKLEKPSMLKPVQVDFFRSVSGAENIQTDDVSRARFSCGKFYGELLDLRLGL
                     VPDPPDAVVAPRTHEEVKKIISFCSGEGIAVVPAGGLSSVTGAVRAPGGGIALDLTKH
                     LNKVLAVNKVNKTVTVQAGMYGPALEEKLNRQGYSCGHFPQSFEYSTVGGWISARGAG
                     QASTGYGKIEDMLVSVKVVTPAGVIVTRDFPRMAQGWDIFRLFAGAEGTLGVITEATL
                     HVFNYAPENTTSAAFIFRSFEAAVEAMRIIIQAECGKPHLFRISDPDETDIAFRTGGF
                     EGTLADRALQMLGFKTGSRCLMFVTVEGEKDYARFVIRKIKRKARAGKGLYIGTSPVR
                     KWLEQRYSSAYMRDPLMDLGIMTDTLETAVTWENLLKVWKAAHEYVARRSKAFMMIHI
                     SHVYENGANLYFTFLSPMEKGNEKNDFTTFHRGLVETILEHGGSISHHHGVGRVLGPW
                     MEGHLGRSSMELLRAVKKHLDPNNIMNPGGTLGL"
     gene            complement(2594..4228)
                     /locus_tag="PKK03_10505"
     CDS             complement(2594..4228)
                     /locus_tag="PKK03_10505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNX84873.1"
                     /translation="MMLKRYIEEGGPDKFDLVIVGGGITGAAVAYIAAARGLSVALFE
                     KKDYGGATSAATSKLIHGGLRYLANGELKLVRESLRERRILGNIAPNFVYPLPFLLPM
                     YKRWRGNIWKMLIGMYVYDVLSYDKKDTWDRSKKLENHRLINHRKTIRVEPNLQKENL
                     RNAFYFFDYQSIFPERLTLAFIKSAAEYGASVSNYTPVEGFIFGEGKKITGVRVRDIF
                     TDRTREIHAGLIVNCGGTWADKILSLASGEGEVKHRVKRSEGIHIITKKIAGNHVVSL
                     QKPDGGHMMIMPWREHSLIGTTDKEFLGDPDDYKVSMESIDEIIENVNNNFGRKISRE
                     DIIHAYGGLRPLVDDQTKGSYETSRKYEVYDNAADGIEGMITVEGGKYTTSRSLAREV
                     LRLISAKLNRTLSDPVSDNLYLSGSEIRDMKQFMIRQHLNYKDFSRNTVEYVSRNYGT
                     ESQVVFRIARSNTRFAEEVTHDGEILAEVVYAIQYENAKTLRDIMLRRTGIGTLGNPG
                     KAVIDKIASLAAGMMGWSEKRKEDEIASLIKVYDLF"
     gene            4368..5555
                     /locus_tag="PKK03_10510"
     CDS             4368..5555
                     /locus_tag="PKK03_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004247678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNX84874.1"
                     /translation="MDGTIIKLMYSLNINGLALSKGRSKRSQFGTFMNITTQKIDVVL
                     SKIVVKMNKKIVPSLESLYSDVRNIIEAARANAYRAVNITMVKAYWNIGRAIVEEEQK
                     GGNRAEYGTFLVENISATLSEEYGKGFDKTNLWNMIRFYRQFPILDALRQELSWTHYR
                     ILLRVEKAPAREFYLQECVDGNWSTRQLERQINSLYFERILMSSKEGRKLVKKEPEDK
                     KENIQPGQLIKDPYVLEFLNLKPATNFYEKEIEQALIDKLKEFLLELGKGFSFVSRQY
                     RISSDTKHFYVDLVFYNYILRCFLLIDLKTGELTHQDIGQMDFYVRFFEDQVRQESDN
                     PTIGLILCAEKDRTIVKYSLLNESKQIFASKYKLYMPTVKELEQEITRERLQIEQEKG
                     LQD"
     gene            complement(5629..6930)
                     /gene="creD"
                     /locus_tag="PKK03_10515"
     CDS             complement(5629..6930)
                     /gene="creD"
                     /locus_tag="PKK03_10515"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HNX84875.1"
                     /translation="METGNEKQEKWYESMTIKMILIAIMGLMLLIPLALIMEVIRERS
                     ANAESARTEIGQLWASAQTVTGPVLNVPGTRVIANDGTFVTTTMHILPGELGVNGVLE
                     PEMRYRGIYETVVYDSEMEISGSFTIAGFEQLNDYTWQWDKAYISLGVSDNKGISDKA
                     ELSIGGRVISAIPGAGQSDLFDKGITFPYAVDPSKLAESGGEFSLKLGLRGSSSVAFS
                     PVGQTTKVTLSSKWNAPSFTGNFLPAERDVTDSGFTASWVVTHLNRSFPQVWEGKRYS
                     PSDDAFGVDLILEADHYTKAERSAKYGLLFIALTFLVLIIIELRSSRRIHIFYYLLVA
                     LALIMFFSLLTALSEHIGFNPAYLISAVATVGLLTFFFRSLLEKGWMTLLISGLLTGL
                     YVFIFFLLAMKDYAFLVGNIGLFVLLAALMIVSARYKLFRS"
     gene            complement(6983..7279)
                     /locus_tag="PKK03_10520"
     CDS             complement(6983..7279)
                     /locus_tag="PKK03_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNX84876.1"
                     /translation="MKQILANFNKAFESRVRLGIMSVLAVNPAMDFTGLKETLAVTDG
                     NLASHLRALEEAGYIRMEKSFINRKPNTSYSITDDGMESFSSHLKALEELIRNQ"
BASE COUNT         1773 a         1895 c         1805 g         1850 t
ORIGIN      
        1 cttcttgaac cagctgatca tccagtaacc cctgtagatc tgcacctcgg tgttgaaaca
       61 gtcggggatg gcgctcgggt atgacgggaa gaatggtatc acctcatgat acctgctgtg
      121 ggtggtctgc accgtagccg tggtgccata gctgagacat gccacatcag ggttgatgac
      181 cccggagccc agaacctcgc tggccttgtc ggctgcagct gctatcagtg gcagcccggc
      241 agggatgcct gacagctccg aagccccgcg actgattgtc cccaacacct cggagggctt
      301 aaccaggtca ggcagcttct cactgtctat cggaaacatc ttgtagttca tatgcttcct
      361 gtcagtccac ttctgggtct tgtaatcgaa cggcaggtaa gccaccgtgc accctaccga
      421 atcgcggtac tctcctgtca gcgcccatgt cagatagccg gagagaagga gatacttgcc
      481 tgtcttcttc catatctccg gttcattctg cataatccag ttgcactcgc cgttcttgac
      541 ggcaaaatga acagcctccc tcatgccaac gagtctcagg gccagatcca tcatctttcc
      601 gggatacttt cccactgcac ttagccgctg gtcgagccag gtaatggccg ggcggagcga
      661 cttcccctca gaatcaaggt tgatcagcgt cgatcgctgc gaagtgagtg tcactgcctt
      721 tatccgatcc ttattgtaag aagtctcgct gaagagcttg cgaagtgact cgaacatctt
      781 gtccctgaag tactcggggt actgttcggc ccacccgggc gccacggaga aataaggctc
      841 gatttcgacc ctggccagtt gaagtacgtt gccttcaagg tcaatgagca cggagcgtac
      901 cgactgtgtg ccgaagtcga tggacaatat caggtcgtca gatgatttca tgtcaggttg
      961 ctaaaggccc agcgtgccgc cgggattcat tatgttatta gggtcaaggt gtttcttcac
     1021 tgccctgagc agttccatcg agctcctgcc aaggtgccct tccatccacg gccccagcac
     1081 tctgcccaca ccgtgatggt gcgagattga gccaccgtgc tccaggatgg tttccaccag
     1141 tccgcggtga aaagttgtga aatcattttt ctcatttcct ttttccatgg gactcaggaa
     1201 ggtgaagtac aggttggcgc cgttctcata aacatgtgat atgtgaatca tcatgaaggc
     1261 ttttgacctg cgtgcaacat attcatgcgc cgccttccag actttcagca ggttctccca
     1321 ggtaaccgca gtctcgagcg tgtcggtcat gatccccagg tccatcagcg ggtcacgcat
     1381 gtaagcgctt gagtatctct gctccagcca cttcctgacc ggagaggttc ctatgtacaa
     1441 ccccttccct gccctggctt tccttttaat cttcctgata acgaaccgtg catagtcctt
     1501 ctccccctcc accgtcacga acatcaggca gcggctgccg gttttgaatc ccagcatctg
     1561 cagtgccctg tcggcaaggg tgccctcgaa tccgcctgta cggaatgcaa tatctgtctc
     1621 gtcgggatcg gagattcgga acaggtgggg cttgccgcat tcagcctgga taataatgcg
     1681 catagcctca acagctgctt caaacgatct gaagataaaa gcggcggagg tggtgttttc
     1741 aggggcataa ttgaagacat ggagcgtggc ttcagtgatc acgcccagtg ttccttccgc
     1801 acctgcgaat agcctgaaga tatcccaacc ctgcgccatg cgtggaaaat cccttgttac
     1861 tatcactccg gcgggtgtca caaccttgac tgacaccagc atgtcttcaa tctttccgta
     1921 ccccgtcgat gcctgtcctg cccccctggc agagatccag cctcccacgg ttgaatactc
     1981 aaatgactgc ggaaagtgac cgcagctgta accctgcctg ttgagcttct cctcgagtgc
     2041 cggcccatac atgcctgcct gcacggtcac agtcttgttg actttgttga ctgccagcac
     2101 cttgttgaga tgtttggtga ggtcaagagc gatgccaccg ccgggtgcgc gtactgcacc
     2161 cgttacagaa gagagtccgc ctgcgggaac cacggctatc ccctccccgc tgcagaaact
     2221 gattattttt ttcacttctt catgcgtacg gggagccacc actgcatcgg gaggatccgg
     2281 tacaaggccg aggcgcaggt caagcaactc accataaaac tttccgcatg aaaaacgtgc
     2341 ccgtgacaca tcatctgtct gtatgttctc cgcacccgaa acactcctga aaaaatcaac
     2401 ctgcaccggt ttgagcatcg aaggcttctc aagtttcact ggctccgttc ccggcagatg
     2461 ttttccttta aaatccgcat ccgacaggtc aaatttctcc ttgatcactt cagccatgtg
     2521 atggctgatg ccctcctcat gcttgtctcc ccacttcaga atgtgacggt aactgttccc
     2581 gttctccata tggtcagaat aaatcgtaaa cctttattag tgatgcaatc tcatcctctt
     2641 ttcttttttc gctccaaccc atcataccgg ccgcaagtga ggcaatttta tcaatcaccg
     2701 ccttgccggg attacccaga gtgccgatgc cggtcctccg gagcatgata tccctgaggg
     2761 ttttggcgtt ctcgtactgt atggcgtaga ccacctcggc cagtatctcc ccgtcgtgag
     2821 tgacctcttc ggcaaacctg gtattactgc gggcaatgcg gaagacaacc tggctctcgg
     2881 tgccgtagtt tctgctcaca tactcaacgg tattgcggct gaagtcctta taattgaggt
     2941 gctgtctgat catgaactgc ttcatgtcac ggatctcact acccgacagg tagagattgt
     3001 cggagaccgg atcagacagt gtacggttga gctttgccga gatcagcctc agcacctcgc
     3061 gtgcaaggct gcggctggtg gtgtacttgc ctccctccac cgtaatcata ccctcgatgc
     3121 cgtcagcggc attgtcatag acctcatact tcctggaggt ctcgtaggag cctttcgtct
     3181 ggtcatcaac cagcggacga aggccgccat aggcatggat tatatcttca cgtgaaatct
     3241 tccggccaaa attgttgttt acattttcaa tgatctcatc gatgctctcc attgacacct
     3301 tatagtcgtc cgggtcaccc aggaactcct tgtcggtggt gccgataagt gagtgttcgc
     3361 gccacggcat gatcatcatg tgacctccgt caggcttctg cagggagacc acatgattgc
     3421 ctgcaatttt cttcgttatg atatgtatcc cttccgaccg tttgacgcga tgtttaacct
     3481 ctccttcgcc ggaggcaagg ctcagtatct tgtcagccca tgtgcctccg cagttgacta
     3541 tcaggccggc atgaatctcc ctggtcctgt cggtgaagat atccctcacc ctgactccgg
     3601 taatcttctt cccctctcca aaaatgaatc cttccacggg agtatagttg gagaccgagg
     3661 caccgtattc ggcagccgac ttgatgaagg ccagtgtcag gcgttcagga aaaatgctct
     3721 ggtaatcaaa gaagtagaag gcattacgca ggttttcctt ctgcaggttg ggttccaccc
     3781 tgatggtttt cctgtggttt atcaggcggt ggttttccag tttcttgctc ctgtcccagg
     3841 tgtctttttt atcgtatgag aggacgtcat agacatacat tcctatgagc atcttccaga
     3901 tattgcctcg ccaccgcttg tacatcggca ggaggaaagg tagcgggtag acgaagttag
     3961 gggctatgtt gccgagaatc cgtcgttcgc ggagcgactc gcgtaccagt ttcagttcgc
     4021 cgttggcgag atagcggagt ccgccatgga tgagtttcga ggtggctgcc gaggtggcgc
     4081 cgccatagtc ttttttctcg aagagagcaa ctgagaggcc gcgcgcagcg gctatataag
     4141 ccacggcagc cccggtgatt cctccgccga cgataaccag gtcaaactta tcaggccccc
     4201 cttcttcgat atacctttta agcatcattg atcagtcagt ttaaaacgac gctgtaagtt
     4261 agtcgaaaaa gccgagaatc ctcatgaggc ggatcaattc attccgcgaa tgaaactcat
     4321 caaccgttag atgattcagg ataatgttcc tttagagatc atcttctatg gatgggacaa
     4381 ttataaaact catgtattct ttaaatatta atggtttagc gttatctaaa gggaggtcaa
     4441 aaagatcaca gtttgggact tttatgaata tcacaactca gaaaattgat gtagttttga
     4501 gtaagatagt agttaaaatg aataaaaaga tagttccttc acttgagagt ttatacagtg
     4561 atgtaagaaa cataattgag gctgcgaggg ccaatgcgta tagggctgta aatattacca
     4621 tggtgaaagc ttactggaac atcggccgtg caattgtaga agaagaacaa aaggggggca
     4681 atcgcgccga atatggtaca ttcctggtag agaatatatc agccactttg tcagaggaat
     4741 atggcaaagg ttttgataag accaaccttt ggaatatgat ccggttttat cgtcagttcc
     4801 cgattctcga cgcactgcgt caagaattat cctggacaca ttacaggata ctcttgcgag
     4861 tagaaaaagc cccggcaaga gagttttacc tgcaggaatg tgtggatggc aattggagta
     4921 caagacagct ggagaggcaa atcaacagcc tatattttga gcggatacta atgagcagca
     4981 aagaaggtcg caaactggta aaaaaagagc ctgaggacaa aaaggagaat attcaaccag
     5041 gacaactaat caaggatcct tatgtacttg aatttttaaa cttgaaaccc gctacaaatt
     5101 tttacgagaa agaaattgaa caggctctta tcgataagct gaaggaattc cttttggaac
     5161 ttggtaaagg cttttcattt gtaagcaggc aatatcgtat atcatctgat acaaagcatt
     5221 tttatgttga ccttgtgttt tacaattata tcctcagatg ctttttgctt attgatctga
     5281 aaaccgggga gttgacacat caggatattg gacagatgga tttttatgtc aggttctttg
     5341 aggaccaggt caggcaggaa tctgataatc caaccattgg acttattcta tgcgctgaaa
     5401 aagaccggac aatagtaaaa tacagtctgt tgaatgaaag caaacaaatc tttgcttcca
     5461 aatacaagct atacatgcca acagttaagg aacttgagca ggagattacc cgggaacgcc
     5521 tgcagataga acaggaaaaa ggattacagg attaatcccc gctgcgagtc gtcgcgcagc
     5581 ggggaaagat cccggccgga ctgccggagg catttcgctc tgcgaaaatt atgacctgaa
     5641 cagcttatac ctggccgaga cgatcatcag cgcggccagc agcacgaaga ggccaatgtt
     5701 gccgacgagg aatgcataat ccttcattgc cagcaggaag aaaatgaaca cataaagtcc
     5761 cgtcagcagg ccgctgatga gcagggtcat ccagcccttt tccagcaacg acctgaaaaa
     5821 gaaggtcaaa agaccgacgg tggccacagc ggaaatgagg taggcaggat tgaatccgat
     5881 atgctcgctc agagcggtaa gcagtgagaa gaacatgata agcgcaagtg ccacaagcag
     5941 ataatagaag atgtgtattc tccgggatga acgcagttca atgattatca gcaccaggaa
     6001 ggtgagagcg atgaaaagca ggccgtattt ggcgctgcgc tcagccttgg tgtaatggtc
     6061 tgcctccaga atcaggtcaa cgccaaaggc atcatcggac ggggaatagc gcttgccctc
     6121 ccacacctgc gggaaactcc tgttaagatg agtcacaacc cagctggctg taaaccctga
     6181 atcagtcaca tcacgttccg caggcaggaa attaccggta aacgagggtg cattccattt
     6241 tgaagaaaga gtaacctttg tggtctgccc caccggagag aaggctacgc tgctgcttcc
     6301 gcggagaccc agtttcaggc tgaactcccc tcctgattct gccagttttg aaggatcaac
     6361 ggcatagggg aacgtgatcc ccttgtcaaa cagatcactc tgccctgcgc cgggaatggc
     6421 gctgatgact cttcccccga tcgaaagctc ggccttgtcg ctgatcccct tgttgtcgga
     6481 gacccccagt gaaatgtaag ccttatccca ctgccaggtg taatcattta gctgctcaaa
     6541 tcctgcaatg gtaaaactgc ccgatatttc catctccgag tcatatacaa cggtctcata
     6601 gatccccctg tacctcatct cgggctccag cacgccattg acaccgagtt cgccgggaag
     6661 gatgtgcatg gtggtggtta cgaaggtgcc gtcgtttgct ataacccgtg ttcccggaac
     6721 attaagaacc ggaccggtta ccgtctgggc cgatgcccag agctggccaa tctcggtacg
     6781 ggctgattcg gcattcgccg aacgctccct gataacctcc atgataagtg caagggggat
     6841 gagaagcatc agccccataa tggcaatcag aatcattttg attgtcatcg actcatacca
     6901 tttttcctgt ttttcgtttc ctgtttccat tttattgagt taattaaagt actttgtgat
     6961 tcaaagttaa attataaaaa aattattggt ttctgatcag ttcctcgagt gccttgaggt
     7021 ggctgctgaa gctctccatc ccatcgtctg tgattgagta actggtgttg ggcttgcggt
     7081 tgatgaaact cttctccatc cttatatagc cggcctcctc gagagcccgc agatgactgg
     7141 ccaggttgcc gtcagtgact gcaagggttt ccttcaggcc ggtgaagtcc atagcgggat
     7201 tcaccgccag cactgacatg atgcccagcc tgacgcggct ctcgaaggcc ttgttgaagt
     7261 ttgcaagaat ctgtttcatc ccctgtacct gagatgcatg aatagtccgt agacgatatg
     7321 cag
//
LOCUS       DAOPXU010000123         7289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_12403_length_7289_cov_11.867432, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000123 DAOPXU010000000
VERSION     DAOPXU010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7289
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12403_length_7289_cov_11.867432"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="PKK03_10525"
     CDS             complement(<1..761)
                     /locus_tag="PKK03_10525"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNX84877.1"
                     /translation="MHRMITRNHVKMILSLQKRKVREDNSLFVIEGDKLVREYILAGN
                     RVMLMAGKPEWIDGESEAVIGGADEVVTVSYEELRRISSLRTPHNVLAVAPMRKAQFS
                     DDSIRGRLTPVLECVQDPGNLGTIIRIAAWFGMGNIICSPDSVDFYNPKVIQATMGAF
                     MHVNVWYRPLDELLRYAAGEGIPVYGTTTDGTSVYESELGSEGIILFGNESKGISAGL
                     LQHVTRRITIPGPPNPVAGLESLNVSMAAAIVCSE"
     gene            825..3185
                     /locus_tag="PKK03_10530"
     CDS             825..3185
                     /locus_tag="PKK03_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNX84878.1"
                     /translation="MIQLRTNIKITGPKLFLLTIIIFTAASCNPTKYVPSGELLLSSN
                     ELTVSPGGESLPATVSRSAMKPYVRQQPNKRIFGVRFHLGLFNLSDITREKWPHGWLR
                     RIGEEPVIFDEVAAERSAGQIQSYLWSKGFFNAIVTDTVNVDGNEAKVIYRVSPGRPY
                     KIADIRYEVQDSILHSLIMIDTVNCSVERGMIYDVDVLRKERQRLERFIRDIGFYTFS
                     TENIFYRIDSSLMSHQMIVNYVVSPGVTFDSQGRQVTTPHKMYRIRDIYVYPEFDPRT
                     ALTGGEEYLNSLDTSYFGGIHFVSPPGRTLVRPEVLAQAIYIAPGSLYSVTNTERTQK
                     HLNDLRNHRLVNVSFIDAGSLSGERRDEGMLDCVIQLTPMQRQSFTVELEGTNTGGNL
                     GGALNLIYQNKSLLKGAEVFSMKLKGAYETLTEDLSGFRNTQQYGIEANLRIPKFMIP
                     FPAKESFIRNRDPRTVLSAGYNYQKLPAFARSVANISLGYSWNGNKYTRFSVNPLSLN
                     VVKLHYINPDYQATIEDMPYFSNSLRDVRIVGGRYDFAFNNQVIQKSRDFWNIRLGLD
                     IAGNLLDAAYRTANAELAPDGSYHLFRQPFAQFIKGEVDAAYNYRFNEASSIVYRFFA
                     GVGWPYGNSEVMPFEEQYFGGGSNDIRAWMVRTLGPGSYILQESSFINQTADMKLEAN
                     VEYRFKLFWILEGAAFIDAGNIWTVREDEDRPGSVFRFRSFTDDIAVGTGLGLRFDLK
                     FVLLRADFGLKVRDPQETEGSKWIPFNRPYDRKKDVTMVIGIGYPF"
     gene            3347..4405
                     /locus_tag="PKK03_10535"
     CDS             3347..4405
                     /locus_tag="PKK03_10535"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNX84879.1"
                     /translation="MKRFRKSVTALFGFDRRERHGTFVLSLIVVTLLLVRLTVMRPGE
                     VPAELPPLPLAQDVNAGGSDTESTTPALFMFDPNTCTYDDLLSLGLSARQASTLINYR
                     GSGARFRRPEDLRRIYGIDSSTAARLIPYILIETDPEGKKSGTRKGWSGADSGRDASV
                     TWANRKESAPERKPSADQEENAVGGPRYNQGDNQATPTAGGGVLALDINRCTAGELVA
                     LPGIGPVLAERIIKYRNLLGGFVDKWQLVEVYGLDSSVARLAAEQLILTFEEVRPLVL
                     DSASYSDLAHHPYLGYETARKITTYRSVSGLPVTLGEMVSSGVITPVQAGRIAPYVRP
                     APGVTGTDYEFISSKVLK"
     gene            4455..4646
                     /gene="rpsU"
                     /locus_tag="PKK03_10540"
     CDS             4455..4646
                     /gene="rpsU"
                     /locus_tag="PKK03_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006045671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNX84880.1"
                     /translation="MIIVPVKEGENIDRALKKFKRKFEKTGVIRELRSRQAYVKPSVR
                     RRDEIKKAVYVQQLQREEE"
     gene            4699..5580
                     /locus_tag="PKK03_10545"
     CDS             4699..5580
                     /locus_tag="PKK03_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNX84881.1"
                     /translation="MNTKESFLQYISTEKRYSRHTVTSYKNDIDQFMGWLEEVRPGAD
                     LVSVIHADVRGWMVSLLEGGASAGTVHRKMSALRTLFRYMRRHELITADPMAGLKLPK
                     KPKQLPVFVAEDALAKLLDEFRFGDNFSGLRDRTVVEFLYLTGMRRSELTGLRDADVD
                     LSAGQVRVTGKRDKQRVIPLAAGFVKSLRSYIEAREEKGFSGGWFFVTDRGNKMYDRA
                     VYNIVTRYLAMVTTVEKKSPHVLRHTFATHMLNYGADLNSIKELLGHASLSATQVYTH
                     STFEQLKKIYKQAHPRA"
     gene            5607..5906
                     /gene="raiA"
                     /locus_tag="PKK03_10550"
     CDS             5607..5906
                     /gene="raiA"
                     /locus_tag="PKK03_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNX84882.1"
                     /translation="MNIQIHSLRFDADKKLIDFVTQKVNKLSQLSDDIVSAEVFLRLD
                     KDDERENKITEIKVEYPRGPLFARKQSKTFEEATDLVVDALKRQIVKQKGKMRGE"
     gene            6019..6092
                     /locus_tag="PKK03_10555"
     tRNA            6019..6092
                     /locus_tag="PKK03_10555"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6053..6055,aa:Thr,seq:tgt)
     gene            6141..6223
                     /locus_tag="PKK03_10560"
     tRNA            6141..6223
                     /locus_tag="PKK03_10560"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6175..6177,aa:Tyr,seq:gta)
     gene            6291..6363
                     /locus_tag="PKK03_10565"
     tRNA            6291..6363
                     /locus_tag="PKK03_10565"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6324..6326,aa:Gly,seq:tcc)
     gene            6370..6441
                     /locus_tag="PKK03_10570"
     tRNA            6370..6441
                     /locus_tag="PKK03_10570"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6402..6404,aa:Thr,seq:ggt)
     gene            6485..>7289
                     /locus_tag="PKK03_10575"
     CDS             6485..>7289
                     /locus_tag="PKK03_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNX84883.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITMVLAKKGLSEV
                     RDFDSIDNAPEEKERGITINTAHVEYSTANRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     IIVVAATDGPMPQTREHILLARQVNVPRIVVFLNKVDLVDDEELIDLVEMEVRDLLTF
                     YKFDGDNAPVIRGSALGAMNHEPKWEDKVMELMDAVDSYIPIPPRENEKPFLMPVEDV
                     FSITGRGTVATGRIETGMVHTGDELEIVGLGSEKRKTVCTGVEMFRKILD"
BASE COUNT         1851 a         1800 c         1938 g         1700 t
ORIGIN      
        1 aattccgaac acacaatggc agcggccatg ctgacgttaa gcgattccag cccggcgaca
       61 ggattcggcg gaccgggaat ggtgatgcgc ctggtgacat gttgcagcag cccggcggaa
      121 attccttttg actcgttccc gaagaggatg atgccctcgc tgcctagctc actctcatag
      181 accgatgtac cgtcagtggt ggtaccgtaa acagggatgc cctcaccggc ggcgtagcgg
      241 agaagctcat caagcggcct gtaccagacg ttcacatgca tgaaggcacc cattgtggcc
      301 tggatcacct tgggattgta aaagtcaact gaatcgggag aacagatgat gttgcccatc
      361 ccgaaccacg cggcaattcg aatgatggtc ccgaggttgc ccggatcctg tacgcactcc
      421 agcaccgggg taagcctgcc ccggatggag tcatcgctga actgcgcctt cctcatgggg
      481 gccacggcca gtacgttgtg cggggtccgg agcgagctta tccgtctcag ctcctcgtag
      541 cttaccgtga caacctcatc agccccgccg ataactgctt cagattcacc atcaatccat
      601 tcaggcttgc ctgccatcag cataaccctg ttgcctgcaa gaatatactc cctgaccagc
      661 ttgtcgccct caatgacaaa gagtgagtta tcctccctta cctttcgttt ctgaagtgag
      721 agtatcattt ttacatggtt gcgggtgatc atcctgtgca taattcacta attgaaaata
      781 gagtttataa tggtatattt gcgtgcacca ctcttcccgg gcaagtgatc caactgagaa
      841 caaatataaa aattaccgga cccaaactct tcctgcttac catcattatt ttcacggctg
      901 catcgtgcaa tcccacaaaa tacgtccctt cgggcgagct gctcctcagc agcaatgaac
      961 ttaccgtcag tccgggtggt gaatcattgc ccgcgacggt gagccggagc gcaatgaagc
     1021 cttatgtcag gcagcagcca aacaagagga ttttcggggt gaggtttcac ctcggactct
     1081 tcaatctctc tgatatcacc cgtgaaaaat ggccccacgg ctggctgagg cggatcggcg
     1141 aggaaccggt gatatttgat gaggtcgccg cagaaagatc cgccgggcag attcagagct
     1201 acctctggtc aaaaggcttt ttcaatgcca ttgttaccga tacggtcaat gtcgacggta
     1261 acgaggcgaa ggttatctac cgggtcagcc cgggaagacc ttataagatt gctgacatca
     1321 ggtatgaggt gcaggattcc attctccaca gcctgataat gattgacacg gtaaactgct
     1381 ccgtggagag agggatgatc tatgatgtgg acgtcctcag gaaggagcgt cagagactcg
     1441 agaggtttat ccgcgacata ggcttttata ctttttcgac ggagaatatc ttctaccgca
     1501 tagacagctc cctcatgagc catcagatga ttgtcaatta tgtcgtttcg ccgggggtga
     1561 cgtttgacag ccagggcaga caggtgacaa ctcctcacaa aatgtaccgg atcagggata
     1621 tctatgtcta tcctgagttt gatccccgga cggcgctgac gggcggcgaa gagtatctca
     1681 acagccttga tacctcatat ttcggaggga tacattttgt atcacctccc ggccgtaccc
     1741 tcgtcaggcc cgaggttctc gcacaggcaa tatatattgc accgggatcc ctctacagtg
     1801 tcaccaatac agaaaggacg caaaaacacc tcaacgatct aaggaaccac aggctggtga
     1861 atgtctcgtt catcgatgca ggaagccttt caggcgaaag aagggatgaa gggatgcttg
     1921 actgtgtcat tcagctaacc ccgatgcagc ggcaatcatt caccgtggaa ctggagggta
     1981 ccaatacagg cggaaacctc ggcggagcac ttaaccttat ataccagaac aagagcctgt
     2041 taaaaggcgc tgaagtattc agcatgaagc tgaaaggtgc atacgaaacg ctcaccgaag
     2101 atctcagcgg tttcaggaac acccagcaat acggcatcga ggctaacctg agaatcccca
     2161 agttcatgat cccatttccg gcaaaagaat cgtttatcag gaacagggat cccagaacgg
     2221 ttctgtcggc cgggtacaac taccagaagc tgccggcatt tgcccgctcg gttgcaaaca
     2281 tatcgcttgg ttattcatgg aacggcaaca aatataccag gttcagtgtg aacccgctct
     2341 cgctgaacgt ggtaaagctt cattacatca atcctgatta ccaggcgaca attgaagata
     2401 tgccatactt ctccaactca ctgcgtgacg tcaggattgt cggcggacgg tatgacttcg
     2461 ccttcaacaa ccaggtgatt cagaaatcac gcgacttctg gaacattcgt ctcgggctgg
     2521 atattgccgg caacctgctt gatgccgcct accggaccgc caatgcagag ctggctcctg
     2581 acggaagcta ccacctgttc cgtcagccgt ttgcccagtt catcaaggga gaggttgatg
     2641 cagcctataa ctacaggttc aatgaggcca gcagcatcgt ttacaggttc ttcgccggag
     2701 tgggatggcc ctatgggaac tcggaggtga tgccctttga ggaacagtac ttcggcggcg
     2761 gatcaaacga tatcagggca tggatggtga gaacgctcgg acccggatct tacatattgc
     2821 aggagagctc attcatcaac cagacagctg atatgaagct cgaagccaac gtcgaatacc
     2881 ggttcaaact cttctggata ctggaggggg ctgccttcat cgacgccggt aacatctgga
     2941 ccgtcaggga ggacgaggac aggcccggaa gcgtcttccg gttcaggtca ttcaccgatg
     3001 acattgccgt aggtaccggc cttggtctga ggtttgacct taagtttgtc ctgctgcgtg
     3061 ccgatttcgg gctgaaggtg cgtgaccctc aggagacgga gggctcgaaa tggatcccct
     3121 tcaatcgtcc ctatgaccgt aagaaggacg ttacgatggt gatcgggata ggatatccct
     3181 tctagcaggc aagaggcaat agccatcagg caatagtgtt gacaagatat gagcagtaac
     3241 tgcctgataa ccaatactta cactactgcc tactgcccat tgcctattgc ctattgcaag
     3301 gtatcccaaa ttttcatatc tttcatgacg tcttgctgct tcgtctatga aacgattcag
     3361 gaaatccgtc acagcactgt tcggttttga ccgccgggag aggcacggaa cgtttgtcct
     3421 gtcactcatt gtcgtgacgc ttctgctcgt acgccttaca gtcatgcggc ccggtgaggt
     3481 gccggcagag ctgcctccgt tgccattggc gcaggatgtc aatgccggcg gcagtgatac
     3541 ggaaagtact accccggcgc tcttcatgtt tgaccccaac acatgcacct atgatgacct
     3601 tctgagtctc ggcctgtcgg ccaggcaggc gagcactctt ataaactacc ggggcagcgg
     3661 ggcgaggttc cgccggccgg aggacctgcg gcggatatac ggaatcgatt cgtccactgc
     3721 cgcaaggctg ataccatata tattaataga gacagacccg gaagggaaaa aatccggtac
     3781 ccgtaaagga tggtccgggg cagattccgg gagggatgca tcggtgacat gggcaaacag
     3841 gaaggagagt gctccggagc gaaagccatc ggcagatcag gaggaaaatg ccgtgggagg
     3901 tccgcggtac aaccagggag ataaccaggc aacccccact gccgggggag gtgtgttggc
     3961 gcttgacatt aaccggtgca ctgccggcga gctcgtcgcc cttcccggca tcgggccggt
     4021 acttgcagaa cggataataa aataccgcaa ccttcttggc gggtttgttg acaaatggca
     4081 gctggttgag gtatacggcc ttgacagctc ggtagcaagg ctggcagcgg agcagctgat
     4141 actgacattt gaagaggtaa ggccgctggt ccttgacagc gcaagctaca gcgatctcgc
     4201 tcaccatccc tacctggggt acgagactgc aaggaagatt accacctaca ggtcggtatc
     4261 cggactgccg gtcacactcg gagagatggt cagcagcggt gtcatcaccc cggttcaggc
     4321 cgggcggata gccccttatg tgagacctgc cccgggcgtt acaggaaccg attatgaatt
     4381 tatttcatca aaagttttga aatgattttc agagatatta attttgcggc tcgattaaaa
     4441 tttaaagttc aaatatgatt attgtaccgg taaaagaagg cgagaacatc gacagggctc
     4501 tcaagaaatt caagaggaag tttgagaaga ccggagttat ccgtgagctc aggtcccgtc
     4561 aggcctacgt caaaccctcg gtccgccgca gggatgagat aaagaaggcc gtctatgttc
     4621 agcagctgca gcgagaggag gagtaatact cctgccggcc gaagctgtaa taaaatataa
     4681 aaccaaaagg ttcctttcat gaatacaaag gaatcgttcc ttcaatacat cagtaccgag
     4741 aagcgctatt cacggcacac tgtaacatct tacaaaaatg atattgatca gttcatgggc
     4801 tggcttgagg aagtccgtcc gggggctgat cttgtatccg taatccatgc tgatgtgcgc
     4861 ggctggatgg tatcgcttct tgaggggggc gcttcggccg gcacggttca caggaagatg
     4921 tcggcgctca ggactctctt ccgttatatg cgaaggcacg agctcattac tgcagatccg
     4981 atggctgggc tgaagcttcc caaaaagccg aaacagcttc ccgtatttgt tgccgaggat
     5041 gctctcgcga agctgcttga cgaattcagg ttcggagata atttttcagg gctccgtgac
     5101 agaactgttg tggaattttt gtatcttaca ggcatgagga ggtcggagct gacaggcctg
     5161 cgtgacgcgg atgtggatct ctctgcgggg caggtcaggg ttactggtaa aagggacaag
     5221 cagagggtca ttcctcttgc tgcaggattt gtaaaatcac ttcggtcata cattgaagcg
     5281 cgtgaggaga aggggttctc aggaggctgg ttctttgtca ctgacagggg gaacaaaatg
     5341 tatgacaggg ctgtttataa catagtgacg agatatcttg ccatggttac aacggttgaa
     5401 aagaagagcc ctcatgttct caggcatacc tttgccacgc acatgcttaa ttacggtgct
     5461 gacctcaact cgataaagga gcttctgggc catgcaagtc tatcggccac gcaggtatac
     5521 acgcacagca cgtttgaaca acttaagaaa atctataaac aggctcatcc aagagcataa
     5581 tcattaactt aacaggaggt actgccatga acattcagat ccattcgcta aggtttgatg
     5641 cagacaagaa actgattgac tttgttactc aaaaggtaaa caagctttct caattaagtg
     5701 acgatattgt cagcgccgag gtgtttctga ggttagataa agatgatgag agggagaaca
     5761 agattacgga aatcaaggtg gagtatccca ggggcccgct ttttgcccgc aagcagagca
     5821 aaactttcga ggaggcgacc gaccttgttg ttgacgccct taagcggcag atagtcaaac
     5881 agaagggaaa gatgcggggg gagtaatccc tctgcatcaa aaaaacttcg gataaatttg
     5941 agatttgaat ttttaatttc tacatttgca aaccgatttt tgagggtaat ttctcagaaa
     6001 tcggtcgttc tttcaaatgc cgatgtagct cagttggcca gagcagctga tttgtaatct
     6061 gcaggtcaag ggttcgaatc ccttcatcgg ctcattgtat gacaggcatg tatgtctaac
     6121 caggcatgct ctgtaaagac ggggagatac caaagtggcc aactggggca gactgtaaat
     6181 ctgttgtctg atgacttcgt aggttcgaat cctgctctcc ccactcttac agtgcaacgt
     6241 tcttttttcc gcagaaacgg gaaaaaaagg agtaccggtt aactatataa gcgggagtag
     6301 ctcagttggt agagcattag ccttccaagc taagggccgc ggattcgaat tccgtctccc
     6361 gctcagcatg ccgatgtagc tcaggggtag agcacttcct tggtaaggaa ggggtcatga
     6421 gttcaattct catcatcggc tcaaacgatt ttggatgtaa atttaaatta ataagctttt
     6481 agttatggca aaagaaaaat ttgacaggtc gaagccacac gtaaatatcg gtacgatcgg
     6541 tcacgtcgac cacggcaaga ccaccctgac ggctgctatt acgatggttc tcgctaagaa
     6601 gggtctctca gaagtaaggg actttgattc aatcgacaat gctcccgaag aaaaggaaag
     6661 gggtattaca atcaacacag cgcacgttga gtactcaact gcgaaccgtc actatgctca
     6721 cgttgactgt ccgggtcacg ctgactatgt gaagaacatg gttaccggtg ctgcccagat
     6781 ggacggcgcc atcatcgttg ttgccgccac tgacggcccg atgccccaga cacgtgaaca
     6841 tatccttctt gcacgccagg tgaacgtgcc gaggatcgtc gtgttcctca acaaggttga
     6901 ccttgttgat gatgaggaac ttattgacct cgttgagatg gaagtacgtg atctcctcac
     6961 attctacaag tttgacggtg acaatgctcc cgttatccgc ggttcagctc tcggtgcaat
     7021 gaaccatgag ccgaagtggg aagacaaggt tatggaactg atggatgcag ttgactcata
     7081 cattcccatt cctccgcgtg agaacgagaa gcctttcctg atgcctgttg aagacgtttt
     7141 ctcaatcacc ggccgtggta cagttgctac cggccgtatc gaaaccggta tggttcacac
     7201 cggcgacgag cttgagatcg tcggccttgg ttcagagaag cgcaagactg tttgtaccgg
     7261 cgttgaaatg ttccgcaaga tccttgacc
//
LOCUS       DAOPXU010000124         7285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_12409_length_7285_cov_13.706777, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000124 DAOPXU010000000
VERSION     DAOPXU010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12409_length_7285_cov_13.706777"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="PKK03_10580"
     CDS             <1..739
                     /locus_tag="PKK03_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNX84884.1"
                     /translation="DQIDMSRLPGHVAVIMDGNGRWAHRRGRERVFGHHEGVKAVRKT
                     VEASAELGVRYLTLYAFSTENWNRPDDEVSALMDLMIESINNETGTLVEKSISLRVIG
                     DLSRLSEKVRNRLFETMKITSQGSRMTLVVALSYSSRWEISEACRRIAVEVREGRMRP
                     DQIDEHTVSTFLTTSDIPDPELMIRTSGEKRISNFLLWQLAYTELYFTEKLWPDFGKE
                     DFYKAVADFQKRERRYGKTSEQIAENS"
     gene            741..3263
                     /gene="bamA"
                     /locus_tag="PKK03_10585"
     CDS             741..3263
                     /gene="bamA"
                     /locus_tag="PKK03_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNX84885.1"
                     /translation="MKKMKPMRILSLFALLLTGVIATAQTEEKYVSYTYPEEYTLAGI
                     TVSGVKFLEPNALIGISGLRIGQKIDIPGEVITAAAKKLMDQGLFSDVRITVTGKEGE
                     NIWLDIYLQERPRLSSLRFEGIRKSEETDLTEKLSLPAGSQVTAHLLNRAERTIRDHY
                     IEKGFLNTAVNIIQKDDPDMPNNVILNFVIDKKAKVKIEQIVFEGNEYFMDKRLRRVM
                     KNTKQKDLNIFKGSKFIEDKFEEDRAKLVEFYNENGFRDFRILGDSTYALEDGKMALV
                     LKVNEGNQYYLRNVDWVGNSIYTQDQLSRVFNFPSGEIYNQTLIDKRLNVDDDAVSAL
                     YLNYGYLFSRLTPVEKKVEGDSIDLEVRIYEGDPATLNNIIITGNTRTNEHVARRELF
                     TYPGDLFSKDKLIRSARQVAVLGHFDPEKINPQPLLNQVDGNVDLLYALEEKANDQFE
                     ISGGWGAGMLVGTVGVRFNNFAIRNFFKWNEWRPYPSGDGQSLQIRAQSNGSYYQSYN
                     VSFIEPWLGGKKPNTLSISAFRNKMGDSKKTPKEDRQSMIIDGGSVGFGKRLEWPDDY
                     FSVFYEASYQRYSLNKYEMYSFLFKNGTSNLATLSAKLTRFSAGPNPIYPTGGSTFSL
                     SLQATPPYSAISGKNFAGASDEEKYKLIEFYKAVFKADYYLPLDGAGKLILNAKAAFG
                     YLGFYNKDIGPSPFENFYLGGDGMTGYSFYGREVIALRGYTNGSLTPRVDKMETDGTL
                     RRVESGNVYSKLTMELRYPISLNPQATIFALAFVEAGKAWYDIRDYNPFRMNRSAGVG
                     LRANLPMFGLLGIDWGYGFDSPVYGSSEDSGSQFHFVIGQQF"
     gene            3290..3805
                     /locus_tag="PKK03_10590"
     CDS             3290..3805
                     /locus_tag="PKK03_10590"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNX84886.1"
                     /translation="MRKIIIMTVIVFAATLSGMAQKFAFVDSEYIRNNIPAFTAAQQQ
                     LDKLSQGWEKEVADGYAAVEQMYKDYQAEVVLLSQDQKKKREETIIAREKEVKDLQNR
                     YFGMEGELYKKREELVKPIQDEILKAIKEISVEGSYAVIFDTSAGSNILFANPRYDLS
                     DQVLQKMGYKN"
     gene            3845..4357
                     /locus_tag="PKK03_10595"
     CDS             3845..4357
                     /locus_tag="PKK03_10595"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNX84887.1"
                     /translation="MKKVFGILLFAALFLTAQDGFAQSFKTGHINRDDIIMAMPDYDT
                     AMKSYNAFGQELTNALELMQVELNNKYDQFTKEEKNLTDLVKANRAQEITDMQTRISN
                     FQQQAQVQLQEKQAELLNPIIEKANNAINAVAKEGGYIYIYDVRTLVYVDTVKSTDIG
                     PLVKTKLGIK"
     gene            4415..5221
                     /gene="murI"
                     /locus_tag="PKK03_10600"
     CDS             4415..5221
                     /gene="murI"
                     /locus_tag="PKK03_10600"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNX84888.1"
                     /translation="MEASNRPIGVFDSGVGGLTVAQAIRQILPGEQIVYFGDTAHLPY
                     GDKSEEAIRGYSRKITEFLLGYDAKVVLIACNSASASAYEALLAEYGNRVIILDVINP
                     VVEYCASRNFQKLGVIGTKLTVNSGTYTRKLKEASPATSVVSLATPLLVPMIEEGFIF
                     DDISNAIIRTYLSDQRLNDINGLILGCTHYPIIRNQISKFFNFSVEVVDSARIVAVRL
                     REILGEHNLVNRGKPGEDLFFVSDFTDYFEKIARMFFESSINLRRADIWS"
     gene            5424..7277
                     /locus_tag="PKK03_10605"
     CDS             5424..7277
                     /locus_tag="PKK03_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNX84889.1"
                     /translation="MGKRSKVIEKDAVVIRFSGDSGDGMQLTGTLFSNTSALFGHDIS
                     TFPDYPAEIRAPQGTVGGVSGFQVHIGHKLINTPGDFTDILVAMNPAAIRANARWLRK
                     GSTIIYDSDNFTEKSIQRAGYKADPFAEDKLDSMNLIAAPISTLVKEALKDMEIDPKT
                     VLRTKNMFALGMVYWLFDSKLKYTEDFIEEKFGKKPLVAEANSKVLRAGYYYGETIEA
                     LTPSYRVDPAPIRKGTYRNINGNQATAWGVLAAAEKAGLKLFIGSYPITPATGVLEEL
                     ALRKDMGVKSLQAEDEIAGICTSIGASFAGRMAVTSTSGPGLALKSEALNLAVMTELP
                     LVVIDVQRGGPSTGLPTKTEQSDLWQALYGRNGESPVPVIAAGTPSDCFRFGFEAAKI
                     AVEHMTPVILLTDSYLANGSEPWRIPSTKDLPPITVPYAVKTDKEFLAYERDPVTLAR
                     KWAIPGTPGLEHRVGGLEKTVKGSVSYTPENHEYMVKIRDEKVERIVKEVPDLKVLGK
                     KTGDLLVIGWGSSYGHITTAVTELQQEGYAVSAAVFNYIRPLPKNTAKVFAGFDKLVV
                     CELNTGQFANYLRMKHQKYTYNQYNKVQGLPFTVKEIKDYCVKLLKGKKNG"
BASE COUNT         2030 a         1804 c         1878 g         1573 t
ORIGIN      
        1 ggatcagata gacatgagca ggttgccggg gcatgtggct gtcattatgg acggcaacgg
       61 ccgctgggca cacagaaggg gccgcgaaag ggtcttcggc catcatgagg gagtcaaggc
      121 agtccgtaaa acagtggagg catccgctga actgggagtc aggtatctta ccctttatgc
      181 cttctctacc gagaactgga acaggcctga cgatgaggtc tctgccctga tggacctgat
      241 gatcgagtca ataaacaatg agaccggaac ccttgtggag aagagtattt ccctcagggt
      301 catcggtgac ctttcacgcc tctcggaaaa agtcaggaac aggctgttcg agacgatgaa
      361 gatcacttca cagggttcgc ggatgaccct cgtggtagcc ctcagctact cgtcgcgatg
      421 ggagatctcg gaggcatgcc ggaggatagc agttgaagtc agggagggaa gaatgcgccc
      481 tgaccagata gacgagcata ccgtcagtac attcctgacc acatcagata ttcccgatcc
      541 cgaactgatg atacgtacca gcggagaaaa gcgcatcagc aatttcctcc tctggcagct
      601 tgcatatact gaactttatt tcaccgaaaa actttggccc gattttggta aggaggactt
      661 ttacaaagca gtggcagact tccagaaaag ggaaaggaga tatggtaaga ccagtgagca
      721 gatcgctgaa aacagttgag atgaaaaaaa tgaaacctat gcggatactc tccctttttg
      781 ccttgcttct gacaggagtg attgccacag cccagacaga ggaaaaatac gtcagctata
      841 cataccccga agagtacact cttgccggca taaccgtttc aggggtgaaa ttccttgagc
      901 ccaatgccct gataggcatc tccggactga ggatcggcca gaagattgat atacccggtg
      961 aggtgattac agcagcagca aagaagctga tggaccaggg gctgttctct gacgtaagga
     1021 taaccgttac cgggaaagag ggcgagaaca tctggctcga catatatctc caggagcgcc
     1081 cgagactctc ctcgctcagg ttcgaaggga tcaggaaatc agaagagacc gacctgacag
     1141 aaaaactaag cctccccgca ggttcacagg taacggcaca cctgctcaac agggccgaac
     1201 gcacaatcag ggatcattat attgagaagg gattcctcaa caccgcagtg aacatcatcc
     1261 agaaggatga tcccgatatg cccaataatg ttatcctcaa cttcgtcatt gacaagaaag
     1321 ccaaggttaa gatcgagcag attgtttttg aaggcaatga atacttcatg gacaagcgcc
     1381 tgcgccgtgt gatgaagaac accaagcaga aggacctcaa tatcttcaag ggctccaagt
     1441 tcatcgagga caagtttgag gaggaccggg ccaagcttgt tgaattctat aatgaaaacg
     1501 gctttcgtga cttccgcata ctgggagact ccacctatgc gctggaagac ggcaaaatgg
     1561 cactggtgct gaaggtcaat gagggcaacc agtactacct gagaaatgtc gactgggttg
     1621 gcaacagcat ctacacacag gatcagctgt cgcgtgtctt caacttcccc tccggcgaga
     1681 tatacaacca gaccctgata gacaagcggc ttaacgtgga tgatgatgcc gtaagtgccc
     1741 tctacctcaa ttacggttac ctcttctcaa ggctcactcc cgtcgaaaag aaggtggagg
     1801 gtgactctat tgacctcgaa gtgcgaatct acgaaggtga tccggcaact ctcaataaca
     1861 tcattattac gggtaacacc cgcaccaatg aacatgtggc caggcgtgaa ctgttcacat
     1921 atccgggtga cctcttcagc aaagataaac tcatacgttc agcaaggcag gtggccgtgc
     1981 tcggacattt cgaccccgaa aagatcaacc cccagcctct cctcaaccag gtggatggaa
     2041 acgttgacct gctctacgca ctcgaggaaa aagccaatga ccagttcgag atatcaggcg
     2101 gatggggcgc tggcatgctc gtgggaactg taggagtgag attcaacaat ttcgccatcc
     2161 gtaacttctt taaatggaac gagtggagac cgtacccatc cggcgacgga cagagtctcc
     2221 agatcagggc acagtcgaac ggcagttatt atcagtctta taatgtctca ttcatcgaac
     2281 catggctggg tggcaagaag cctaacaccc tctccatttc tgccttccgg aacaagatgg
     2341 gtgacagcaa gaaaacacct aaggaagaca ggcagtcaat gatcattgac ggtggttcgg
     2401 tgggcttcgg taagaggctg gaatggcctg atgactattt ctcggtattt tatgaggcca
     2461 gctatcagag gtacagcctc aacaaatacg aaatgtacag tttcctcttc aagaacggca
     2521 cgtccaatct tgcaacactg agcgccaaat taaccaggtt ctcggcaggg cccaacccga
     2581 tttacccgac cggtggttcc accttctcac tttcattgca ggccacccct ccatattcag
     2641 ccatcagcgg caagaatttt gccggtgcca gtgatgaaga gaaatataaa ttgatagagt
     2701 tctacaaggc tgttttcaag gcagattatt acctgcccct cgatggtgca ggaaagctta
     2761 tactcaacgc caaagcagct ttcggatacc tgggatttta caataaggac atcgggcctt
     2821 cgccctttga aaacttctac ctcggcggcg acggcatgac cggatacagc ttctacggcc
     2881 gcgaggtaat agccctcagg ggttacacca acggatcact gactcccagg gttgataaga
     2941 tggagaccga tggaaccctg agacgggtgg agtcaggtaa cgtttactca aagctcacca
     3001 tggaactgcg ctatcccatc tcactgaacc cgcaggcaac cattttcgca ctggccttcg
     3061 tcgaggcagg caaggcctgg tatgatatca gggactacaa tccattccgt atgaaccgct
     3121 cggcaggggt aggcctgaga gcaaacctgc cgatgtttgg cctcctcggc attgactggg
     3181 gttacggatt cgattcacct gtttacgggt catccgaaga ttccggaagc cagtttcatt
     3241 tcgtaatagg tcagcaattc tgatgaaaac aagatcaaat acaacagcaa tgagaaagat
     3301 cattatcatg acagtcattg tgtttgccgc cactttaagc ggtatggcac agaaatttgc
     3361 attcgttgac agcgagtaca tacgcaataa catacctgct tttacagcag cacagcagca
     3421 gcttgacaaa ctttcccagg gatgggagaa ggaggtggct gacggttatg cggcagtgga
     3481 gcagatgtac aaggactacc aggctgaggt ggtgctgctc tcacaggatc agaagaagaa
     3541 gagggaagag accatcattg ccagggagaa ggaggtgaaa gaccttcaga acaggtattt
     3601 cggcatggag ggagagctct acaagaaacg cgaggagctg gtgaaaccta tccaggatga
     3661 gatactgaag gcaatcaagg aaatatctgt cgaggggagc tatgcagtga tttttgacac
     3721 atccgcaggt tcgaacatcc tcttcgccaa cccgagatat gacctctcag accaggttct
     3781 ccagaaaatg ggttataaga attaaataat tacatttgca cgttaaaatt tgaaaattaa
     3841 gacaatgaag aaagtatttg ggatactcct gtttgctgca ctgtttctca cggcacagga
     3901 tggatttgca cagtcgttca agacaggtca catcaatcgc gatgacatca ttatggctat
     3961 gcctgactat gacacggcaa tgaagagtta caatgcattc gggcaggagc tgacaaatgc
     4021 tttggagctg atgcaggttg aactgaacaa caagtatgat cagtttacca aggaggagaa
     4081 gaatcttact gatcttgtaa aggcaaacag ggcgcaggag ataacagaca tgcagacacg
     4141 aataagcaat ttccagcagc aggcccaggt tcagctgcag gagaagcagg ctgagcttct
     4201 gaatcccatc atcgagaagg ccaacaacgc tatcaatgcc gttgcaaagg agggcggtta
     4261 tatatatata tatgatgtcc gcactctcgt ttacgtggat acggtaaaga gcaccgacat
     4321 cggtccgctg gtaaagacga agctgggaat caaataatta aaatgaaatc aaagtgctgc
     4381 ccgcccccgg cgggcagtct tacattataa agttatggaa gcaagcaacc gtccgatcgg
     4441 ggtatttgac tcaggtgtgg gagggttgac ggtggcacag gccatccgcc agatcctgcc
     4501 cggggaacag atagtctatt ttggtgatac ggcacacctg ccttacggcg acaagtcaga
     4561 agaagccatc aggggctatt caaggaagat taccgagttc ctcctcggct atgacgccaa
     4621 ggtggtgctc attgcctgca actcggcatc agcctcagcg tacgaagctc tgcttgcaga
     4681 atacggcaac agggtcataa tcctcgatgt gatcaacccg gtggttgagt attgcgcttc
     4741 acgcaacttt cagaaactcg gagttatagg caccaaactg actgttaact caggcactta
     4801 caccaggaaa ctgaaggagg catctcccgc caccagcgtg gtctcgcttg caacaccgct
     4861 gcttgtaccc atgatcgagg aaggctttat ctttgatgac atcagcaatg ccatcatccg
     4921 cacttatctc tccgaccaga ggctgaacga cataaacggc ctgatactcg gatgcacaca
     4981 ctatcccatc atacgtaacc agataagcaa atttttcaac ttcagcgtgg aggtggttga
     5041 ttccgcccgt atcgttgcag tcaggctccg ggaaatcctc ggggaacata acctggtcaa
     5101 caggggcaaa ccgggtgaag acctgttctt tgtatcagac tttacagatt actttgagaa
     5161 gattgccagg atgttctttg agagctccat caacctccgc agggcagaca tctggtcatg
     5221 atacttaact aattaccctc cacagatata ccggcatatt ttttctgcag gattccgctg
     5281 tccggatgag gttatttgga aggtcatgac aaacatgacc aaaatcatac agttgtatca
     5341 ggttaatggt gaacttagct tcaccttttc aggttactgt tttatacagg ttcttattta
     5401 aaatcaaata catcacaatc atcatgggaa aaagatcaaa ggtcattgag aaggatgcgg
     5461 tggtgatcag gttctcaggc gactcgggag acgggatgca gcttacaggc acccttttct
     5521 ccaacacgtc ggccctgttc ggacatgaca tctccacctt tcctgactat cctgcagaga
     5581 taagggcccc gcagggtacc gtcggaggcg tctccgggtt ccaggtacac atcgggcaca
     5641 aactgattaa cactccgggt gatttcaccg acattcttgt ggcgatgaat cccgcagcca
     5701 tccgcgccaa tgccaggtgg ctgaggaaag ggtccacaat catttatgac tcagataatt
     5761 tcactgaaaa gtcgatacag agggcaggct acaaggctga tccgtttgca gaagacaagc
     5821 ttgactctat gaacctcatt gcggcgccta tctccaccct ggtgaaggaa gccctcaagg
     5881 atatggaaat tgatcccaaa accgtactca ggacgaagaa catgtttgca ctggggatgg
     5941 tttactggct ctttgacagc aagcttaagt acacagagga cttcattgag gagaaattcg
     6001 gaaagaagcc gcttgtggcc gaggccaaca gcaaggtgct cagggcaggc tactactacg
     6061 gagagaccat tgaggcgctg acaccttcat acagggttga tcccgcacca atcaggaaag
     6121 gcacttaccg caacatcaac ggcaaccagg ccaccgcatg gggcgtgctt gccgctgccg
     6181 agaaagccgg actgaagctc ttcatcggtt cataccccat taccccggct accggtgtcc
     6241 ttgaggaact tgctctaagg aaggatatgg gcgtcaagag cctccaggct gaggacgaga
     6301 tcgcaggcat atgcacctcc atcggagcaa gcttcgcagg gcgcatggcc gtcacatcca
     6361 catccggtcc cggactcgca ctgaagtctg aagcccttaa cctggccgtg atgactgagc
     6421 tgccactggt agtgattgac gttcagcgtg gcggaccctc cacgggactg ccgaccaaaa
     6481 cagaacaatc tgacctgtgg caggcactct acggccgtaa cggcgaaagc ccggtaccgg
     6541 tcattgcagc cggaacacct tcagactgct tcaggttcgg gttcgaggct gcaaaaattg
     6601 ccgtcgaaca catgacaccg gtgatcctcc ttactgacag ctatctggcc aacggctcgg
     6661 aaccttggag gataccatca accaaagacc ttcctcccat cactgtacct tacgcagtta
     6721 aaactgacaa ggagttcctc gcatacgaga gagacccggt gacactggca cgcaaatggg
     6781 ccatccccgg cacaccggga ctggagcacc gtgtgggcgg actcgagaag acagttaagg
     6841 ggagcgtatc atataccccg gagaaccatg aatacatggt gaaaatccgc gacgaaaagg
     6901 tggaacggat tgtaaaggaa gtgcctgatc tgaaggtcct cggcaagaaa acaggcgatc
     6961 tgcttgtaat cggatggggc agcagctacg ggcatattac caccgctgtg actgaattgc
     7021 agcaggaggg atacgcagtg tcggcagccg tcttcaacta catcaggccc ctgccgaaaa
     7081 acaccgcaaa ggtgttcgcc ggatttgaca aactggtggt ttgcgaactg aatacaggcc
     7141 agtttgccaa ctacctgaga atgaagcacc agaagtacac gtacaaccag tacaacaagg
     7201 ttcaggggct cccgttcact gtcaaggaga ttaaagatta ttgtgttaaa ctgcttaagg
     7261 gtaagaaaaa tggctgaaac aaata
//
LOCUS       DAOPXU010000125         7092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_12898_length_7092_cov_13.560608, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000125 DAOPXU010000000
VERSION     DAOPXU010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12898_length_7092_cov_13.560608"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..663)
                     /gene="efp"
                     /locus_tag="PKK03_10610"
     CDS             complement(97..663)
                     /gene="efp"
                     /locus_tag="PKK03_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNX84890.1"
                     /translation="MATTADFRNGMVIEFNNDLYTIVQFQHVKPGKGPAFVRTRLKNV
                     TTGKVLENTFNSGVKVNVARVERRPHQFLYSDDMGYHLMHVETFDQITVPDSMIENSD
                     LLKEGDTVEVVVHADTENVLLVDLPVNVVMEVTYSEPGLRGDTATNTLKPATIETGST
                     IMVPLFVNTGDRIRIDTRDRSYIERVKG"
     gene            complement(686..1468)
                     /locus_tag="PKK03_10615"
     CDS             complement(686..1468)
                     /locus_tag="PKK03_10615"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNX84891.1"
                     /translation="MRKTFIAVLIIITAALQLLDAQKQPYLPGEKVTYQIRYGIVGSG
                     QASLELREGIHKGRPVWHAVISGQTTGIADALYKVRDVYESYIDPENDLPVFSIRNIQ
                     EGRYRKYNEVGFDHVSGADDSTLVFSDLTGDHKGPEGLLDIVSCFYWFRKYYLASGKE
                     LVPGETYRMMTWFADELYPIILKYKGKETIRVKGKRIECYRFHPVTEVGRVFKTEEDM
                     AMWFTADENFLPVKIRFDIFVGSFYVDMTSYEGLRAPLRFID"
     gene            complement(1472..2176)
                     /gene="tsaB"
                     /locus_tag="PKK03_10620"
     CDS             complement(1472..2176)
                     /gene="tsaB"
                     /locus_tag="PKK03_10620"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNX84892.1"
                     /translation="MILCIETATGVCSAALCDEKRVHGVAEAPPGMSHAAQLTVIIQQ
                     LMKENRITATDLDAVAVSKGPGSYTGLRIGVSAAKGIAYGAGIPLIGINTLAAMCSGY
                     ISRHPDDLRPGTLLCPMIDARRMEVYNAIFAADGTRVTETSASIIDKNSFSEFLAENQ
                     VIFFGNGSGKCRGIIAGPNAFFRDDFSLSASYLFIPSVNAMRNRQFEDLAYFEPYYLK
                     EFIATVPKSFFPDKPE"
     gene            complement(2199..3467)
                     /locus_tag="PKK03_10625"
     CDS             complement(2199..3467)
                     /locus_tag="PKK03_10625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX84893.1"
                     /translation="MKNNRLLKILAAAVVLLLIFAVVGKKAGWFGKEVTIKVATEKVG
                     VNPIIEAVTANGKIQPETEVKISPDVSGEIVELHVKEGDFVQKGTLLFKIKPEIYISS
                     RDRAAATLNSTKSRLAQVEAQMIQAELAFNRSKKLFEENTISKADFEQAESQYKVAKA
                     EMESAQYSVKSSEASLKEANENLIKTTVYSPMTGTISSLLVEQGERVVGANMMTGTEV
                     LRVADLNRMEVVVDVNENDIIRVKLGDTAIVEVDAYLDRDFKGVVTEIANSANTLGTT
                     SDQVTNFKVRILILKESYEDLISEKNPSPFRPGMSASVDIYTSSKANVMTVPIQAVTT
                     RTDTTITDPAAKNDIRTLVFVSDGTYALARDVTTGIQDNVNIEILSGLTGEEDVIVQP
                     FTAISKKLSDSTRIEVVAKEALFSTEKTKK"
     gene            complement(3488..4855)
                     /locus_tag="PKK03_10630"
     CDS             complement(3488..4855)
                     /locus_tag="PKK03_10630"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX84894.1"
                     /translation="MKRKTLYLGFILLLGPLALFSQQKQWTLEECIAHALQNNISIKQ
                     QEIMTEYQVAALEQSKLNLLPTLNGSASHNYAFGRALDETTYEFTENETVQSNNFYAG
                     SNVTLFRGLVSYNTIQKNKYEVLASEQELEQFRDNISLNISLAYLQILLNQELVAATE
                     SQVELTKQQIDRTRKMVDAGSLARGNLLEIEAQAAREELQLVNMRNQLTLSLLNLAQM
                     LELQNTDDFSVAIPAISVRDEAVDGNPAAIYSIAEKTRPEIMSAEYKLKSAEYDLAIA
                     KGNRSPRLSLGASMSTGYSDMRLKPMTNEKYPFGEQLNDNLNYGLGFSLNIPIFNGWQ
                     VNTGIKNSRLGIENSRYALENTRKQLYKNIQQAYTDASGALKKYNASQKAVTSMEEAF
                     RYAEQKLGVGMVTAVEYNQSKTQLLNAQSEMAQAKYEYVFKTKVLDFYKGIPLTLDHI
                     TILAK"
     gene            5022..6098
                     /gene="fbaA"
                     /locus_tag="PKK03_10635"
     CDS             5022..6098
                     /gene="fbaA"
                     /locus_tag="PKK03_10635"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNX84895.1"
                     /translation="MTNKGLNLEPGVISGEDVKKLFDHANKNDFAIPAVNVVGTDSVN
                     AVLETAREVKSPVIIQFSNGGAHFFAGQGLTKENHTGAIAGGISGALHVHNVAEYYGI
                     PVILHTDHAARKLLPWIDALLDAGEEFYESNGKPLFSSHMLDLSEEPLKENIETCKKY
                     LERMDKMGMTLEIELGVTGGEEDGVDNTGVHSSKLYTQPEDVAYAYEELMKVSHNFTI
                     AASFGNVHGVYKPGNVTLRPEILKNSQDYIQKKFKTGPNPVNFVFHGGSGSEKHLIAE
                     AIKYGAIKMNIDTDMQWAFWSGVKDYYEAKKGYLQGQIGNPEGEDKPNKKYYDPRVWL
                     RKGQETFIARLKEAFADLNAMNRL"
     gene            6234..6476
                     /locus_tag="PKK03_10640"
     CDS             6234..6476
                     /locus_tag="PKK03_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84896.1"
                     /translation="MRRGPLTEEKIKRTTTLTLRLNEREAKALSIYCKRYRVRSKSDF
                     MRRTIMQAIVVRFDAEHPSLWEQGELTLFTHEAKNN"
     gene            6555..6938
                     /locus_tag="PKK03_10645"
     CDS             6555..6938
                     /locus_tag="PKK03_10645"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HNX84897.1"
                     /translation="MELEIIKIEPTRVTPQVLFLEGHIELRGRSISENSTDFYRPLAA
                     WVDSYVEKTEVRTRVILAFDFINTASTKWIYSIVKRLALYPNVREMVSIEWYYEKGDE
                     DQYELGQIIHSFIDCPFIFYETEQT"
     gene            6986..7058
                     /locus_tag="PKK03_10650"
     tRNA            6986..7058
                     /locus_tag="PKK03_10650"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7019..7021,aa:Gln,seq:ttg)
BASE COUNT         1631 a         1671 c         1843 g         1947 t
ORIGIN      
        1 gcgttgatgg agagggtaat gtcaagcagg gcgtcgagct tgaacttcga catcttaagt
       61 ctgtccttcg gaagcctgcc ttctctcatt tccacgtcag ccctttaccc tttctatgta
      121 ggagcggtca cgcgtgtcaa tgcgtatcct gtcgccggta ttgacaaaga gtggcaccat
      181 gatggttgac ccggtctcga tagttgccgg cttcagggtg ttggttgctg tatcaccgcg
      241 gaggcccggc tccgaatagg ttacttccat taccacgttg accggaaggt caaccaggag
      301 aacattctcc gtatcagcgt gcaccaccac ctcaaccgta tcgccctctt tcaggagatc
      361 actgttttca atcatcgagt cgggaacggt tatctgatca aatgtctcga catgcatcag
      421 gtgatagccc atatcatcac tgtaaaggaa ctggtggggt ctgcgttcca cccttgccac
      481 attgaccttc actccggagt tgaatgtatt ttcaagcacc tttccggttg taacgttctt
      541 aagtctcgtt cttacgaagg ccgggccctt tcccggtttt acatgctgaa actgaacgat
      601 ggtataaagg tcattgttga attctatcac catcccgttt ctgaaatctg ctgttgtagc
      661 cataaagaaa aataattcat gttggttagt caataaatct gaggggcgct ctaagccctt
      721 catagcttgt catatccacg taaaatgacc ccacaaagat atcaaatcgt attttaaccg
      781 gtaaaaaatt ttcatcggcg gtgaaccaca ttgccatatc ctcctctgtc ttgaagactc
      841 tgccaacctc ggttacggga tggaaacggt agcattcgat tcgtttcccc ttcaccctga
      901 tagtctcttt tcccttgtat ttcaggataa taggatatag ttcgtcagcg aaccatgtca
      961 tcatcctgta tgtctctccc gggacaagct cctttccgga agcgagataa tatttcctga
     1021 accagtaaaa gcaggagaca atgtcaagga gtccctccgg tcctttatgg tcacctgtga
     1081 ggtcgctgaa gacgagggtt gagtcgtcag ctcctgaaac gtggtcaaat ccaacctcgt
     1141 tgtatttgcg gtagcgtccc tcctggatat tccttatgga gaacaccgga aggtcatttt
     1201 ccgggtcaat gtagctttca tatacatccc tcaccttgta gagcgcgtcg gcaatgccgg
     1261 tggtttgtcc ggagatcact gcatgccaga ccggcctgcc cttgtggatt ccctccctga
     1321 gctcgaggct tgcctgaccg ctgcctacga taccatatct tatctggtaa gtcacctttt
     1381 cacccgggag gtaaggctgc ttctgggcat caagcaattg cagggctgca gttattatta
     1441 tcaggaccgc aataaatgtt tttctcattt ctcattcagg tttgtcaggg aagaagcttt
     1501 tcgggactgt ggcaatgaac tccttgagat aatagggttc gaagtaagcc aggtcctcaa
     1561 attgcctgtt cctcattgca tttaccgatg ggataaacaa atatgatgcc gagaggctga
     1621 agtcatccct gaagaaggca ttcgggccgg caattatgcc gcggcatttt ccggatccgt
     1681 tcccgaagaa aatcacctgg ttttcagcga ggaattcact gaatgagttt ttgtcaataa
     1741 tgcttgcgga agtttccgtg acccgggttc cgtcagcggc aaaaatggca ttgtatactt
     1801 ccatgcggcg ggcatcgatc atcgggcaca ggagtgttcc gggccggagg tcatccgggt
     1861 gacggcttat gtagccgctg cacattgcag ccagggtgtt tattcctatc agcggtatgc
     1921 cggcgccgta agctatgcct ttggcggctg agacgcctat cctcagtccg gtgtatgagc
     1981 ccggtccctt gctgacagca accgcatcaa ggtctgttgc cgtgatgcgg ttttctttca
     2041 tgagttgttg aataatgact gtgagctgtg cggcatgaga catgcctggc ggggcctcgg
     2101 caacgccgtg cacccttttc tcgtcgcaca atgcggcaga gcatacaccg gtggctgtct
     2161 caatgcagag aatcattcag ttatgtcagc agattatctt acttcttcgt cttttcagtg
     2221 ctgaaaagag cctccttcgc aacaacctct atgcgtgttg aatcagacag tttcttggag
     2281 atggcggtga aaggctgaac tattacatct tcttccccgg tgagtccgct caggatctct
     2341 atgttgacat tatcctggat gccggtcgtc acgtcgcgcg caagcgcata ggttccgtcc
     2401 gaaacaaaca ccagggtgcg tatatcattt ttggcggcag ggtcggtaat ggttgtgtca
     2461 gtgcgggtgg tgacagcctg gatgggcact gtcatcacat tggccttgct gctggtgtaa
     2521 atgtcaaccg aggcagacat gccgggcctg aacgggctgg gattcttttc agagatcagg
     2581 tcttcatatg attccttgag gatgagtatc ctgaccttga agttggtcac ctggtcagag
     2641 gtggttccga gggtgtttgc cgagttggct atttcagtca caacgccctt gaaatcacgg
     2701 tcaagatagg catccacctc aacaatggca gtatcaccga gcttaaccct gatgatgtca
     2761 ttttcattga cgtcaaccac cacttccatg cggttcaggt ctgcaacgcg gaggacctcg
     2821 gtacctgtca tcatgttggc gcccacaacc ctttcgccct gttcaaccag caggcttgag
     2881 atggtgcctg tcatgggtga atagacagtg gtctttatca ggttttcatt ggcttccttg
     2941 agtgatgcct cagagctctt gacagagtac tgggcgctct ccatctcggc tttggccacc
     3001 ttgtattgtg actctgcctg ttcaaagtcg gcctttgaaa ttgtgttttc ctcgaacagc
     3061 ttcttgctcc tgttgaatgc cagttcagcc tggatcatct gtgcttccac ctgtgccagg
     3121 cgggattttg tcgagttgag ggtggccgca gcacggtcac gcgatgagat atagatctct
     3181 ggttttatct tgaatagcag ggttcccttc tggacgaaat cgccctcctt gacatgaagt
     3241 tcgacgatct cgccggatac atcggggctg atcttgacct cagtctcggg ctgtattttg
     3301 ccatttgccg tcaccgcctc aatgattgga ttaacaccca ccttttcagt ggcaaccttt
     3361 atggtcacct ctttgccgaa ccagccggcc ttcttgccca cgacggcaaa tatgaggagg
     3421 agaacgacgg cagcggcgag gattttaaga agtctgttat ttttcatcgg ttgaatgttt
     3481 actggaatta tttggcaagg attgtaatgt ggtcaagtgt cagcgggatg cctttataga
     3541 aatcgaggac cttggttttg aagacatact cgtattttgc ctgggccatt tcgctctggg
     3601 cgttgagtag ctgtgtcttt gactggttgt attccacagc ggtgaccatt cccactccga
     3661 gtttctgttc ggcatagcgg aatgcctctt ccatggaggt gactgccttc tggcttgcgt
     3721 tgtatttctt gagagccccg cttgcatcag tgtatgcctg ctggatattc ttgtaaagct
     3781 gtttcctggt attttcgagg gcataccttg aattttcgat cccgagcctg gagtttttta
     3841 ttccggtgtt gacctgccag ccgttgaaga tggggatgtt aagtgagaat ccgagtccgt
     3901 aattcaggtt atcattcagc tgttccccga acgggtattt ctcattggtc atgggcttga
     3961 gcctcatatc tgaataacct gttgacattg aggcgccgag gctcagccgc gggctcctgt
     4021 tgcctttggc tattgcgagg tcatattcag cgcttttgag tttgtattca gcgctcatta
     4081 tctccgggcg tgtcttctct gctatcgaat agattgctgc aggattaccg tctactgcct
     4141 cgtccctcac cgatatggcg ggtatggcta ctgagaaatc atcagtattc tgcaactcaa
     4201 gcatctgggc caggtttaga agtgacaggg taagctggtt tcgcatgttc accagctgca
     4261 gttcctcgcg tgcggcctgt gcctctatct caagcaggtt gccgcgcgcc aggctgccgg
     4321 cgtctaccat cttgcgggtc cggtcaatct gttgttttgt gagctccacc tgtgattccg
     4381 tggctgccac cagctcctgg ttgaggagta tctggagata ggccagggat atgttcagcg
     4441 agatattgtc cctgaactgc tcaagctcct gttcactcgc cagcacctcg tacttgttct
     4501 tttggattgt gttgtagctc accagccccc tgaaaagggt aacattgcta ccggcgtaga
     4561 agttgttcga ttgtacggtt tcgttctcgg tgaactcgta agtggtctcg tcaagtgcac
     4621 gcccgaaggc ataattgtga gatgcgctac cgttgagggt cggaagcagg ttaagcttcg
     4681 actgttcaag cgctgcgacc tggtactcgg tcatgatctc ctgctgcttt atgctgatat
     4741 tgttctgaag ggcatgtgca atgcactcct ccagcgtcca ttgcttttgc tgggagaata
     4801 gcgcgagcgg acccagcaac agaataaagc ccaggtaaag agtctttctt ttcataatct
     4861 gattgacagc attatttaag tgtacgaaga taggaaatag atcagtattt taaagactgc
     4921 aaagatatta tgatgttgca taaggtctgt caacagtatc attcattttt gtatatcttt
     4981 gcgcagcttg actacaaaaa aataatcaaa tttataaaac gatgactaac aagggattaa
     5041 atctggagcc gggtgtgatc agcggcgaag atgtgaagaa gctgtttgac catgccaaca
     5101 agaatgattt cgccattcct gccgttaatg tggtaggaac cgattctgtc aacgctgtac
     5161 ttgagactgc acgtgaggta aaatcaccgg ttatcataca gttttccaat ggcggtgcac
     5221 atttctttgc aggccaggga ctgaccaagg agaatcatac gggggcaatt gccggaggca
     5281 tatcgggtgc actgcacgtg cacaatgtgg cggagtatta cggcataccc gttatactgc
     5341 atactgacca tgctgcccgc aagctcctgc cctggattga cgcgctgctt gatgctggcg
     5401 aggagtttta tgagtcaaac ggcaagccgc tgttcagctc tcatatgctt gatctctcgg
     5461 aggagccgct gaaggagaac attgagacat gcaagaagta tcttgagagg atggacaaga
     5521 tggggatgac ccttgagatt gaactcggtg tcaccggcgg cgaggaagac ggtgttgaca
     5581 acaccggcgt tcacagctcg aagctctaca cccagcctga ggatgtggca tatgcatatg
     5641 aggaacttat gaaggtttca cataacttta ccattgccgc atcattcggt aacgtacacg
     5701 gtgtgtacaa gcccggtaac gtaacgctca ggcccgagat actgaagaat tcgcaggatt
     5761 atatccagaa gaaattcaag accggaccca atccggtgaa tttcgtgttc cacggcggct
     5821 caggatcaga aaaacacctg atagccgagg ccattaagta cggtgccatc aagatgaaca
     5881 ttgacactga catgcagtgg gcgttctggt caggtgtcaa ggattactac gaggccaaaa
     5941 agggttacct gcagggccag ataggcaatc ctgagggaga agacaagcct aacaagaagt
     6001 attatgatcc gagggtatgg cttcgcaaag gccaggagac cttcattgca aggcttaagg
     6061 aggcgtttgc cgaccttaac gcaatgaaca ggctgtaaga cttattaata agctaacata
     6121 aagcaggtca gcgcattggc gttggcctgt ttttttattg ttgataagtt gcatttttca
     6181 ttggcccaca ggatggccat gcgggctact ttagcataaa attgactgcc ggattgagac
     6241 gcggaccttt gactgaggaa aagataaaga ggaccaccac gcttaccctc aggctgaatg
     6301 agagggaggc gaaggcgctc agcatctact gcaagcggta cagggtaagg agcaagtcgg
     6361 atttcatgcg gcggaccatc atgcaggcca ttgtcgtgcg gtttgatgcg gaacatccgt
     6421 cattgtggga gcagggtgag ctgaccctgt tcactcatga ggcgaaaaat aactgaaaag
     6481 ggataggggc aactcttatt ttattatttt tgtgattcca cgatagcggg ggaaggtgtc
     6541 gtctcggaag aaaaatggaa ttggaaatta ttaaaataga acccaccagg gttacccccc
     6601 aggttttatt ccttgagggt catattgagc tcaggggcag gtcgatatct gagaattcaa
     6661 ctgattttta caggccgctg gcagcatggg ttgactccta tgttgagaag accgaggttc
     6721 gcacgagggt tattcttgca tttgatttca taaacacggc ctcgacgaaa tggatttatt
     6781 ccattgtcaa gcggctggct ctttacccga atgtccgtga gatggtttcc attgaatggt
     6841 attatgagaa gggtgacgaa gatcagtacg aacttgggca gataattcac tcttttatcg
     6901 attgtccgtt catcttttac gagactgagc agacctgata atccggaaaa atatttattt
     6961 ttgccacgcc ttatcaggcg ccgattgacc catggtgtaa ttggcaacac gtctgatttt
     7021 ggttcagaag agtcggggtt cgagcccctg tgggtcaaca aaagaaagag agtgtgattg
     7081 agagcgagca ct
//
LOCUS       DAOPXU010000126         7084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_12913_length_7084_cov_11.252383, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000126 DAOPXU010000000
VERSION     DAOPXU010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12913_length_7084_cov_11.252383"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PKK03_10655"
     CDS             <1..266
                     /locus_tag="PKK03_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84898.1"
                     /translation="WHNSFLINFPFYSGLQKFMFNTIILSELACTLPLPFSVRGNISQ
                     AGTPLPPFLLRASARITQPVHYGGKGLPQCPGVKFSEDVTYGL"
     gene            485..2641
                     /locus_tag="PKK03_10660"
     CDS             485..2641
                     /locus_tag="PKK03_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNX84899.1"
                     /translation="MKTYSTDHLRNIALLGNSGSGKTTLAEAMLFNGGVIERRGTVEA
                     RNTVSDYKPIEQENRNSIYSTVLYTEFRDKKINILDTPGLDDFSGGVISSMYAADTAM
                     LVINVQNGVEVGTEIHFRHAERFHKPLIIAVNGLDHEKANFEKTVEMLHERFGSNVTL
                     AQFPVNEGLGFNTVIDVISMKMLRYGKDGGKAEVLDIPAEHKAKADELHAALTEKVAE
                     SDEALMELFFANDKLSEEEIGRGLREGLLKRGIFPVFFVSSKGNIGIDRLLDFVAVSA
                     PSPADMPAVKNSKGTEVKCTAAGPASVFVFKTTLEEHIGEINYFRVMSGKVTENFDAV
                     NTSNGTKERLSQIFACAGKNRTRIPEIHAGDIGGFVKLKNTKTNHTLNAPGNDWTYEE
                     MMFPAPKYRAAIKPLSESDEEKMGEALNRMHQEDPTILVEYSKELKQIIIHGQGEYHL
                     NILKWHLDNIYKIETQFLAPKIPYRETITKASQADYRHKKQSGGAGQFGEVHMIIEPY
                     EEGSEAKTMMKIAGKEIKLSNRDLEEIELPWGGKLIYVNCIVGGSIDARFMPAILKGI
                     MEKMEVGPLTGSYARDIRVYVYDGKMHPVDSNEISFRLAGRNAFSTAFKNAGPKILEP
                     VYDVEIWVPSDRMGDVISDLQGRRGMVLGMSSEKGFEVIKARVPLAEMNKYSTALSSL
                     TGGRAMYNMKFSEYSQVPGEVQDAVIKAYESEEEEE"
     gene            complement(2727..4646)
                     /locus_tag="PKK03_10665"
     CDS             complement(2727..4646)
                     /locus_tag="PKK03_10665"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX84900.1"
                     /translation="MKKQNWYKLIYQFAIIAILAFMGFRLLFDKAYAPDFEAYCPFGG
                     LQALGSYMTMDSLSCSMTSTQIMMGVALFIGIVLFSRLFCGYICPLGTIGEWTGKLGD
                     RLKVRITLKGIADKALRLLKYVLLFITFYFTLKSSELFCKKFDPYYAVASGFDTDVVL
                     LWALIAIGALLVGSLFFRLFWCRYLCPLGALSAIFKYSWWFLGVMAVYVILLLLGLNI
                     SYVYPLLIVTAGGYILEVTRMDRVRPDPVHITRNTGTCTSCGLCTESCPQGIDVASME
                     KVTHADCTLCGDCLHACPEKDTLQINKRNMKWLPAVVLGALIILGITAGSLFELPTIS
                     QQWGTEEQLANAGTFEMSGLKNIKCFGSSTAFANQMRSVEGIYGVSTYVASHTVHILY
                     DTAMYNDEKIQQLIFVPVKRVIEEPSADVSNIVYHTLTIDKFFDPLDATYLQHLLDQK
                     TEAVGYQSDFACPVIVRIYFPEGKAPDRETLIKAIESKNLRFTVNETEFNVKLPYRVV
                     TLEENSGSMPVNEFAREMFMPVKSFFNEYQKYTPDVLKGMPVRLGDNAQLKQRYNYLI
                     SHISNDEGVVGFETLLNDAGEEMVTLWYVDTLTVPARIFSVMNADSLLLHYGDGTTGT
                     VPNPFRFAPADTLPH"
     gene            complement(4866..5159)
                     /locus_tag="PKK03_10670"
     CDS             complement(4866..5159)
                     /locus_tag="PKK03_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84901.1"
                     /translation="MKAIMIIYNLALTEKVEYTLEHFGIRGYTQWDNVEGVGSFTGVP
                     HLGTHTWPEINGVTLTFVDEDKVAPLLEAVQRIDAINKEVGIRAFVWSVEQAV"
     gene            complement(5156..>7084)
                     /locus_tag="PKK03_10675"
     CDS             complement(5156..>7084)
                     /locus_tag="PKK03_10675"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX84902.1"
                     /translation="IERGSRPREAAIYATNEVWLAVIVTTLTVVAVFLPLTFVKGLTG
                     VLFAQLGWIVSITIIMSTLAAITLTPTLSALLLRLRPVKENAPFWTWDGSVHKFLEWL
                     DGFYVRTLAWVLHHKKFVILGALVIFIASMMLFKFIGTEFMPSADESRVSATVELQTG
                     TRVTETEKVADRIDSIVRKQIPEIKLISMSAGTDDSGGFTSLFGQSGTHVVTFTFALV
                     DVEDRKRSSEEVAEEIRGIISPMPEVINFSVLSEGGGMGAMGGGNTVDIEIYGFDISQ
                     TNLLAEQLAEKVKKIPGATNVDISRDKSKPELQIILDQDKLLRYGLTTAQVSTAIRNR
                     VDGLTATRLRQFGDEYDVIVRYKESARNSITELQNIGVALPTGQVIRLGEIATVKEYW
                     SPPNIERKRKERIVTVSVTPYKRALNLIVADIQKELDALTIPSGVMVEISGAFEDMSE
                     SFMDIGLLMVISLILVYLVMASQFESLKMPVIIMLSIPFAFSGVAIALFLTGTNLSLI
                     AAIGAIMLIGIVVKNAIVLVDFINLTRDRGVELYQAVLISGRSRLRPVLMTSLTTILA
                     MLPLAINPGEGSELWQPMGIAVIGGLVFSTIVTMVLVPVGYVLIARHGERDKKHKVAF
                     RDMKFLDEIKENHENGKS"
BASE COUNT         1772 a         1860 c         1806 g         1646 t
ORIGIN      
        1 gttggcataa ttctttctta attaattttc ctttttattc tggtcttcaa aaattcatgt
       61 tcaacactat catcctctcc gaactggcct gcactctgcc tctccccttt tctgtccggg
      121 gcaacatttc tcaggccggc acacctcttc ccccattttt gcttcgtgcg tcagcgcgga
      181 tcacacaacc cgtgcattat ggcggcaaag ggctgccaca atgccccggg gtaaagtttt
      241 ctgaagatgt aacttatggt ttgtaagtta tttccaggat tttcttcccg accgctcctt
      301 ccgacatctt ttgtaaactt acacactgac ggtgcgaccg gtactcctgt tttcaaaaaa
      361 ccgccgcaaa gaaagtaaat atttttcaaa caaccatcac tttatatgca atcttttgaa
      421 aatttgttat ttttgcgctc cattccgtac agaatacatt atagtaatat aacaacagcc
      481 gaagatgaag acatactcaa cagaccacct aagaaacatt gctctgctgg gaaactctgg
      541 ctcaggtaag accaccctcg cggaagcaat gcttttcaac ggcggggtta tcgagagacg
      601 gggtacagta gaagccagga acaccgtctc tgattacaaa cctattgagc aggagaaccg
      661 gaactccatc tattcaacag tactttacac cgagttcagg gacaagaaga tcaatatcct
      721 tgacacacct ggccttgacg atttctccgg aggggttata tcatcaatgt acgctgctga
      781 caccgccatg ctggtgatca acgtgcagaa cggcgttgag gtgggcacgg aaattcactt
      841 caggcatgcc gagaggttcc ataaacccct catcatagcc gtcaacggcc tcgaccatga
      901 gaaggcaaac ttcgaaaaga ccgttgaaat gcttcatgag cgtttcggaa gcaatgtgac
      961 acttgcacag ttcccggtga acgaaggtct cggcttcaac accgtcatcg acgtcatcag
     1021 catgaagatg ctgcgttacg gcaaggatgg cggcaaagcc gaagtacttg acatcccggc
     1081 cgaacataag gccaaggccg atgaactgca tgctgcactc accgaaaagg tagccgaaag
     1141 cgatgaggca ctgatggaac tcttctttgc aaacgataag ctttcagagg aagaaatcgg
     1201 acgcggactc cgcgaaggac tcctaaagag gggtatcttc ccggtgttct ttgtatcatc
     1261 aaagggaaac atcggtattg acaggctgct cgattttgtg gcagtatccg ccccgtcacc
     1321 cgcagacatg cctgcagtga agaactcgaa gggtaccgag gtaaaatgca ctgctgccgg
     1381 tcctgcttca gtatttgtct tcaagacaac cctcgaggaa cacatcggcg agatcaacta
     1441 cttcagggtg atgtcaggca aggttaccga gaactttgat gcagtgaaca ccagcaacgg
     1501 caccaaggaa cgcctctccc agatcttcgc atgcgccggc aagaaccgca cccgcattcc
     1561 cgagatccac gcaggtgaca tcggtggctt cgtcaaactg aagaacacaa aaacaaacca
     1621 taccctcaac gctccgggga acgactggac atatgaggaa atgatgttcc cggctcctaa
     1681 ataccgtgca gccatcaagc ctctcagcga aagcgatgag gaaaagatgg gtgaggccct
     1741 taacaggatg caccaggaag acccgacaat cctcgtcgag tattccaagg aactcaagca
     1801 gatcatcatc cacgggcagg gtgaatacca tctcaacatt ctgaagtggc atcttgacaa
     1861 tatatacaag atagagaccc agttccttgc accaaagatc ccttaccgtg aaactatcac
     1921 gaaggcatca caggccgact atcgtcacaa gaaacagtca ggtggcgcag gccagttcgg
     1981 tgaggttcat atgatcatcg agccgtacga ggaaggttcg gaggcaaaga ccatgatgaa
     2041 gattgccggc aaggagatta agctttccaa ccgtgacctg gaggagatag aactaccctg
     2101 gggcggcaag cttatctatg taaactgtat tgtgggaggt tccattgatg cacgtttcat
     2161 gcctgcgatc ctcaagggta ttatggagaa gatggaggtt ggtcctctga caggctccta
     2221 tgcccgtgac atcagggtct acgtctatga cggcaagatg cacccggttg actcaaacga
     2281 aatttcattc cgtctggcag gccgtaacgc cttcagcacc gcgttcaaga acgccggtcc
     2341 gaagatcctc gagcctgttt acgatgttga gatatgggtt ccgtcagacc gtatgggtga
     2401 tgtgatcagt gacctgcagg gacgccgcgg aatggttctc ggcatgtcaa gcgagaaggg
     2461 attcgaggtc atcaaggcaa gggttcccct ggcagagatg aacaagtact cgacagccct
     2521 gagttctctc accggcggtc gtgcaatgta caacatgaag ttctccgaat attcacaggt
     2581 tcccggcgag gttcaggatg ccgtcatcaa ggcatacgaa tccgaggaag aagaggaata
     2641 acaaccacag atccaataat ctgtataaca ggcacgtagc ggcaggtctc agacctgccg
     2701 ctacctgtat atggcggttg catgcatcaa tggggcaacg tgtctgccgg agcaaaacgg
     2761 aacgggttgg gcaccgtgcc cgtggttccg tctccgtaat ggagcagcag gctgtcggcg
     2821 ttcatgacgc tgaatatccg ggcaggaaca gtcagcgtgt caacatacca cagggtcacc
     2881 atctcctctc cggcatcgtt gagcagcgtc tcaaaaccca cgaccccttc gtcattagag
     2941 atatgactta taaggtaatt gtatctctgc ttcagctgag cattatcacc cagccttaca
     3001 ggcatcccct tcagaacatc aggtgtatat ttctggtatt cattgaagaa cgacttcacc
     3061 ggcatgaaca tctcccgtgc aaactcattc accggcatgc ttccgctgtt ctcttccagg
     3121 gtcacaaccc tgtaaggaag cttgacatta aactcggtct cattgacagt gaaacgcagg
     3181 ttcttcgatt caatggcctt tatcagcgtc tccctgtcag gggccttccc ttcggggaaa
     3241 tagatacgta caatcaccgg gcatgcaaag tcgctctggt aacccactgc ctccgtcttc
     3301 tggtcgagca ggtgctggag gtaggtggcg tcaagcggat cgaagaactt gtcaatggtc
     3361 agggtatggt aaacaatgtt actgacatct gcagaaggtt cctcaatcac ccgtttcacg
     3421 ggaacgaata tcagttgctg gattttttcg tcattgtaca tcgcagtgtc atacagaata
     3481 tgtaccgtgt ggctggcgac gtaagtggat acgccataga tcccttctac gcttctcatc
     3541 tggttggcga aggcggtgga gctgccgaag cacttgatgt tcttcagacc ggacatctcg
     3601 aaagtccctg cgtttgccag ctgttcttcg gtgccccatt gctggctgat ggtgggcagc
     3661 tcaaacagtg agccggcggt tatgccgagg atgatgagag cgccaagcac cactgccggc
     3721 agccacttca tgttccgttt gtttatctga agggtatcct tttcagggca tgcatggagg
     3781 cagtcgccac acagtgtgca gtcggcgtgc gtcaccttct ccatggaagc aacatcaatg
     3841 ccctgcgggc atgattcggt gcagagtccg caggaggtac aagtgccggt attccttgta
     3901 atatgtaccg gatcaggacg caccctgtcc atacgggtca cctcgaggat ataacctccg
     3961 gcagttacta tcagaagcgg gtagacatat gagatattta accccagaag cagcagtatc
     4021 acataaactg ccatcactcc caggaaccac catgaatact tgaagattgc cgagagggca
     4081 ccaagaggac agaggtaccg gcaccagaag agcctgaaga agagagatcc gacaagcagg
     4141 gctcctattg ctatcagggc ccataacagg accacgtctg tgtcaaagcc cgacgccacc
     4201 gcgtaatagg gatcaaattt cttgcagaag agttcgctcg atttaagagt aaagtagaag
     4261 gttatgaaaa gcagtacata ctttaggagc cgcagggcct tgtcagctat tcctttcagg
     4321 gtgatccgga ccttcagcct gtctcccagc ttgcctgtcc actcccctat tgttcccagc
     4381 gggcagatgt atccgcagaa gaggcgggag aatagcacga tacctatgaa gagcgccact
     4441 cccatcatta tctgtgtgct ggtcatggag catgaaagcg agtccatggt catgtagctt
     4501 cccagcgcct ggaggcctcc gaacgggcaa taggcttcaa aatcgggtgc gtaggctttg
     4561 tcaaacagga ggcggaatcc catgaacgcg agaattgcga taatggcaaa ctgataaatc
     4621 agcttgtacc agttttgttt cttcattttg gaggagttag ggtacaagcc aaatatatca
     4681 ttttcatcca gaaaggcaaa tgacaaaaag cagcagcgat gtcccgggtg gaaacatcgc
     4741 tgctgtttga taactggcag gggtacggcg tcagccgctt cgccccgcag gggtaatgcc
     4801 cggatcagca gacccggatg tcttcaggga cagagccggg agcaaccatt ccggatgccg
     4861 ctgcgtcaga cagcctgttc tacagaccag acgaatgccc tgatacccac ctccttgttg
     4921 atggcgtcaa tgcgctgcac cgcctccagc aggggggcaa ccttatcctc gtcaacaaag
     4981 gtgagggtca ccccgttgat ctcgggccag gtgtgggtgc cgaggtgagg cacaccggta
     5041 aaggagccca ccccttccac attgtcccac tgggtgtaac ccctgatgcc aaaatgctcc
     5101 agcgtatact ccaccttttc agtaagagcg aggttgtata taatcattat tgctttcatg
     5161 actttccgtt ttcatggttt tccttaatct cgtccaggaa cttcatatcc ctgaatgcca
     5221 ccttatgctt cttgtccctt tcgccgtggc gggcaatgag aacatagccc accggcacaa
     5281 gcaccatcgt tacgatggtt gagaagacca gcccgccgat aacagcgata cccatcggct
     5341 gccacagctc ggaaccttcc cccgggttaa ttgccagcgg gagcattgca aggatggtgg
     5401 tcagggaggt cataagcaca ggcctgagcc tggaacggcc tgaaatcagc actgcctggt
     5461 aaagctccac gcccctgtca cgcgtaaggt tgatgaagtc taccagcacg atggcgttct
     5521 tgacgacgat accgatcagc attatagcgc cgattgcggc tatgaggctg aggttggtgc
     5581 ctgtcaggaa taaggcgatt gccacacctg agaaggcgaa tggtatcgag agcatgatga
     5641 tgacaggcat cttcagcgac tcaaactgcg aggccatgac caggtaaacc agtatcagcg
     5701 agataaccat cagcagcccg atatccatga atgattcgga catgtcttca aaggctccgg
     5761 agatttcaac catcacaccg gagggtatcg tcagggcgtc aagctccttc tgaatatcgg
     5821 ctacgataag gttcagtgct ctcttgtagg gtgtgactga aacggttaca atgcgttcct
     5881 tgcgcttcct ctctatgttg ggaggcgacc agtattcctt gacagtggct atttcgccca
     5941 gcctgatcac ctggccggtg ggaagggcca ccccgatgtt ctgaagctcg gtgattgagt
     6001 tacgggctga ttccttatac cttactatca cgtcgtactc atccccgaac tggcgcaggc
     6061 gtgtggcagt cagcccgtca acgcggttgc gtattgctgt cgagacctgt gctgttgtca
     6121 gcccgtatct cagaagcttg tcctgatcaa gtatgatctg aagctccggc ttcgacttgt
     6181 cgcggctgat atcgacgttt gtggcgccgg ggatcttttt gaccttctcg gcaagctgtt
     6241 ctgccagaag gttggtctgg gagatgtcaa atccgtatat ctctatgtca acggtgtttc
     6301 ctccgcccat ggcgcccatg ccgcctccct ctgaaagcac cgagaaatta atcacctccg
     6361 gcatcggtga gatgataccc ctgatctcct cggcaacctc ttcagatgac cttttcctgt
     6421 cttccacgtc aacaagtgcg aatgtgaaag tgacaacatg ggtgccgctc tgtccgaaca
     6481 acgatgtgaa tcctccgctg tcgtccgtgc ctgctgacat tgagatgagc ttaatttcag
     6541 gaatctgttt cctgactatt gaatcgatac ggtcagcaac cttttctgtt tcagtcaccc
     6601 ttgttccggt ctgaagctcg acggtagccg aaacccttga ttcgtcagcc gagggcataa
     6661 attcagtgcc gatgaatttg aacagcatca ttgaagcgat gaatatcacc aatgcaccaa
     6721 gaattacgaa tttcttatga tgcaataccc aggccagtgt cctgacgtaa aatccgtcga
     6781 gccactcaag gaatttatgg acagagccgt cccatgtcca gaaaggtgca ttttccttaa
     6841 ccggtctcag tcggagcaac agggcgctca gtgtcggggt gagggtgatg gcggcaaggg
     6901 ttgacatgat gattgtaatg gatactatcc agcccagctg tgcaaagagc acaccggtca
     6961 gtcccttgac aaaggtcagt ggtaggaaca ctgccacaac ggtaagggtg gtgacaatga
     7021 ctgccagcca cacttcgttg gtggcgtaga tggctgcctc gcgcggacgg cttcccctct
     7081 caat
//
LOCUS       DAOPXU010000127         6960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_13231_length_6960_cov_14.438957, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000127 DAOPXU010000000
VERSION     DAOPXU010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6960
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13231_length_6960_cov_14.438957"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PKK03_10680"
     CDS             complement(<1..789)
                     /locus_tag="PKK03_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84903.1"
                     /translation="MKKLSYSLLLVLAVSITSCTSNRRGSGDAAVVSGDYLGQAAPGD
                     SARLFASGIISTGMTERDFAITPDGNEIFFCREAGNFRYVTIFYSRRSDGVWSIPEVF
                     EFCTDPAYKYVEPHISPDGTRLYFISTMPADSASEANEDIWVCTKTDGRWSEPRNLGA
                     PVNTASKEFFPSVTVDGTIYYTHLDPVSGEEFIYRSKLVNGVFQEPEKLGPNVNTGSA
                     RYNAFVADDESYIIVPAYGMPDSFGATDYYISFRDSLDNWSQPVN"
     gene            complement(820..1542)
                     /locus_tag="PKK03_10685"
     CDS             complement(820..1542)
                     /locus_tag="PKK03_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84904.1"
                     /translation="MKNIIIAMILLVTGSSCSQKPENGYELIEQMYKANKSNWYETLC
                     FTQEVLHYWNDSITSSEVWYEAYQSPGNLIIRFNDWNSGNGMLFTNDTIYSFREDTLE
                     SVRYRIHDLVVLGLDINNIPPEITAERAVKCGYNLDLLAEGKCMGRDAWIAGDTLSTC
                     FWADMESLLFLKMRRRSGANSREVEFARYEDINGFPVATEIRFYDKDGVLNMVEKYFN
                     VMPGCRVPAQTFIPSEFNNARW"
     gene            complement(1597..2517)
                     /locus_tag="PKK03_10690"
     CDS             complement(1597..2517)
                     /locus_tag="PKK03_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84905.1"
                     /translation="MNRIADSPVLIFLATFGLSWVFWTSPVPGTTGILADIRTEKPVP
                     RPEVFIVGSIHSMHYNPDYHYSINDLLEQIIALKPDVVCGEIAPEAFEHITEGYFPPE
                     AALLAEMAPELHYRFVPVDWRLDYATQSKADSIYPESVKEKLAPFGSTYFARMNESAS
                     QSMYDAVHCESNIAIIDSVFEQIIGADPIAETAHGNWHERNRRIAENGMNAAGDAQRI
                     VFVFGSDHIPQIRRQLQILGFEPQVPDRLFIPSGNHKVPDAVLKRWKHNLENLRLIRD
                     RKIPVTDDYFQKIINSGRIRELEQAIQKSS"
     gene            complement(2514..3485)
                     /locus_tag="PKK03_10695"
     CDS             complement(2514..3485)
                     /locus_tag="PKK03_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84906.1"
                     /translation="MIHEETMLRIVKYAVKAPSCHNAQPWRFKIRNDTICLLPDFTRA
                     LPVADNDNHSLYISMGCALENMILAAHEFGYDTEVEITGNGNNPQIIVALHDEPEGVK
                     PELFDYILKRQARRNKYRNDKVPDNLISELFNDPDDEGVSTKLFLSGNEMREMIPYIS
                     EGTRLQFSNKAFVDELVGWVRFSEKEAMLKGDGIRAGAMGLPNTGRLIGKIIMKYFIS
                     AEIEARRMEELARASSGFALFMAEKNDPYHWIRLGQAFQRFGLKAAKYQVSHSHLDMP
                     CEEMEVRDKLINHLQLKDLTPLLLIRFGYSGPMPYSFRRSFYKLLDP"
     gene            complement(3720..4850)
                     /locus_tag="PKK03_10700"
     CDS             complement(3720..4850)
                     /locus_tag="PKK03_10700"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX84907.1"
                     /translation="MEYILIIGTFEALFLLLLLLGKKNKSLPDYFLAAIFVLYAISIG
                     GVYLELQNFSHNFTYSAIISTSWVFLFLHGPALWFYIRSLSDHEFRFKPLYLLHFVPF
                     IFFSVFHYFNFINLTVPEKIRFLENDLFKESVSYKITVMLIGVSTISYNIWALNLIRR
                     RRDRLMQHYSKIEDIDLGWLSALTIATLISYSVNVTLFNLDMIFHFATYNFLIILTNG
                     FASVYILFLGYFGIRQGNIFINQSKEIQKSGVKSALTEASSPVKNTDDAFIKTLTRDM
                     EEKQPYLDPDITISRLSEMLNVKVEFLSEVLNARLNQNFFDFINRYRVGEFKEQCVLK
                     ANSHLSIMGIAYNCGFNSKASFYRAFRKFEGMSPSEYIGKVS"
     gene            complement(4943..5512)
                     /locus_tag="PKK03_10705"
     CDS             complement(4943..5512)
                     /locus_tag="PKK03_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNX84908.1"
                     /translation="MKTRRLSLVFGLLVLATGSAMSQTAGNSVTDLLLSAWSPRNYTE
                     VAVTDQQIDMILQCGIKAPSSRNGQPWHFTVLKNEAMIKEIISNITPGNVIIIISGKV
                     SQSGSTPDFDCGLATQNMFVAATSMGLGARIYGGPIAAAREKREALQIPEGFTPVIML
                     RVGNIEKGTDAVTGASPRKSKEEIVNWVR"
     gene            complement(5662..6435)
                     /locus_tag="PKK03_10710"
     CDS             complement(5662..6435)
                     /locus_tag="PKK03_10710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX84909.1"
                     /translation="MSSENQSIHEIDYGLICEYFSNFERQGPGSPEVTKKALSFIDNL
                     TNDSVIADIGCGTGGQTMVLAQNARGRITGIDLFPKFIELFNANAGKLNLQDRVKGIT
                     GSMDKLPFGDEELDLVWSEGAIYNIGFERGLKEWRKFLKKGGYIAVSESSWFTEERPE
                     EINRFWMDAYAEIDTIPRKVDQMQKAGYVPVAAFILPEECWTVNYHEPQASIQEAFLK
                     KYPGNKAAEDLVAFQRHEVQLYDKYKEYYGYVFYIGRKI"
     gene            6665..>6960
                     /locus_tag="PKK03_10715"
     CDS             6665..>6960
                     /locus_tag="PKK03_10715"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNX84910.1"
                     /translation="MKVTAFIGSGRKRHSYNACEKFLQNLQTMGNVEYEMVRLSEYNI
                     GTCRGCRICFDKGEEFCPHKDDRDKLIEKMKDSDGVIFASPNYSFQVSGMMKVFL"
BASE COUNT         1776 a         1674 c         1593 g         1917 t
ORIGIN      
        1 attgaccggc tgactccagt tgtcgaggct gtcgcgaaag gatatgtaat aatcagtggc
       61 gccaaaactg tcgggcatgc cgtatgccgg tacaatgata taactttcat catccgcaac
      121 aaacgcattg tatctggcac tccctgtgtt tacgtttggc ccaagctttt caggctcctg
      181 aaaaaccccg ttaaccagtt tggagcgata gatgaactct tcgccggaga caggatccag
      241 gtgggtgtaa tagattgtac cgtcaaccgt cactgacggg aaaaactcct tgcttgcagt
      301 atttacgggt gcaccaaggt tccgtggctc cgaccatctg ccatctgttt ttgtgcatac
      361 ccagatatct tcatttgctt ctgacgcgga gtcagccggc attgtggata tgaaatacaa
      421 ccttgtcccg tcaggagata tgtgcggctc aacatacttg tatgcgggat cggtacaaaa
      481 ctcaaatacc tcagggattg accacacacc gtcactcctc cgggagtaaa aaatggtgac
      541 atacctgaaa ttgcctgcct cgcggcagaa gaagatctca ttgccatcag gagtgatggc
      601 gaaatcgcgc tcagtcatac ccgttgagat gatgccagat gcaaagagcc tggcactgtc
      661 accgggagct gcctgcccga ggtaatcacc gctgaccaca gccgcatcac cggatccccg
      721 gcggtttgat gtgcaggaag ttattgaaac ggcgagaacc agtaacaggg agtacgaaag
      781 ttttttcatt ttattataaa tcattgtttt atccggtgat caccaccttg cattattgaa
      841 ttccgacgga ataaatgtct gtgcaggaac tctgcaaccg ggcatgacat taaaatactt
      901 ctcaaccatg ttcaggactc cgtctttgtc atagaacctg atttccgttg caacgggaaa
      961 accattgatg tcctcatacc tggcaaactc aacctctctt gagttagcgc ctgaccggcg
     1021 cctcatcttc aggaacagca aagactccat gtctgcccaa aagcaggtac tcagggtatc
     1081 acctgctatc caggcatcac gtcccatgca ctttccctct gccagaagat cgaggttata
     1141 tccgcatttc acagcccgtt cagcagtaat ttccggtggg atattattta tgtccagtcc
     1201 aagtacaacc aggtcatgta tccggtaccg gacagattcg agtgtgtctt ccctgaagct
     1261 gtagatggtg tcatttgtaa acagcattcc gttgccgctg ttccagtcgt tgaatctgat
     1321 gatgagattg ccgggcgact ggtatgcctc gtaccagact tcggaagatg tgatgctgtc
     1381 attccagtaa tggagaacct cctgcgtgaa gcaaagagtc tcataccagt tcgatttgtt
     1441 tgctttgtac atctgctcaa ttaattcata cccgttttca ggtttctgag agcatgatga
     1501 gcctgtgaca agcaggatca tggcaattat tatattcttc attatcttga ttttttcgtt
     1561 gaagcgctca gggttctcta tcccggagca attgatttat gacgattttt gtatggcctg
     1621 ttcaagttcc ctgatccggc cactgttgat tatcttctga aagtaatcat ccgtgaccgg
     1681 gatttttctg tcgcggatta atcgcaggtt ctccaggttg tgtttccagc gtttcaggac
     1741 ggcatcgggt accttatggt tccctgacgg gataaacaaa cggtccggaa cctgcggttc
     1801 gaatcccagg atttgtaact gtctccttat ctgtggaatg tgatcacttc cgaaaacaaa
     1861 gactattctt tgcgcatccc cggcagcatt catcccgttc tctgcaatcc gccggttgcg
     1921 ctcgtgccag ttgccatggg ctgtttcggc aatcgggtct gcgccaatga tctgttcaaa
     1981 tacagaatca ataatcgcga tgtttgattc gcagtgtact gcatcataca tagactggct
     2041 ggctgattca ttcatcctgg cgaaataggt gcttccgaaa ggtgccagtt tctccttcac
     2101 cgattccgga taaatgctgt ccgctttcga ctgggtggca taatccagcc gccagtcgac
     2161 cgggacgaac cggtaatgaa gctcaggtgc catctctgcc agcaaggctg cctcgggtgg
     2221 gaagtagcct tcagttatat gttcaaatgc ctcaggggcg atctccccgc aaaccacgtc
     2281 aggcttcaag gcaatgatct gctccagcag gtcattaatt gaataatggt agtcagggtt
     2341 ataatgcatg gaatgaatgg atccgactat gaagacctcc ggccggggga caggtttttc
     2401 cgtcctgata tctgcgagga ttcctgtagt gcccggtaca ggagacgtcc agaacaccca
     2461 tgacaggcca aacgtagcca ggaaaatcag gacaggggaa tctgcaattc tattcatgga
     2521 tcaagcaatt tataaaaact tctcctgaat gagtatggca ttggtcccga ataaccaaac
     2581 cggattagca gcaacggcgt caggtctttc agctgcaagt ggttaataag tttatcacgt
     2641 acctccatct cctcacaggg catatccaga tgagaatgac tgacctggta ttttgccgcc
     2701 ttaaggccga agcgctgaaa agcctggccc agtcttatcc agtggtacgg gtcgttcttc
     2761 tctgccatga agagagcgaa tcctgaagaa gcccgggcca gctcctccat acgcctggcc
     2821 tcaatttcgg cagagataaa atacttcatt attatctttc cgataagcct tcccgtattt
     2881 ggtaaaccca tcgcgcctgc ccggatacca tcacctttca gcattgcctc cttctcactg
     2941 aaccgtaccc agccaaccaa ctcgtcgaca aatgccttgt tgctgaattg cagcctggtg
     3001 ccttcagaaa tgtaagggat catctccctc atctcatttc ccgagagaaa cagctttgtc
     3061 gagacacctt catcatcagg atcattaaaa agttcactta tcaggttgtc aggcacttta
     3121 tcatttctgt atttattcct tcttgcctgc cgttttaaaa tataatcgaa caactccggt
     3181 ttgaccccct cgggttcatc atgaagcgca acaatgattt gcggattatt tccattgccc
     3241 gtgatttcaa cctctgtatc ataaccgaat tcatgcgccg caagaatcat gttttccagg
     3301 gcgcacccca tgcttatata taaagaatga ttatcgttat cggcaacagg caacgccctg
     3361 gtgaagtcag gcaacaggca tattgtgtca ttcctgatct tgaatctcca tggttgtgca
     3421 ttatgacatg aaggagcctt cacagcatac ttgacaatcc ttaacatggt ctcttcatgt
     3481 atcatttcaa tgcgcttgat taaaccaggt atccatattt tactggccat aaattaaatg
     3541 cctgactacc agcaggaggt tactcgcctg gattgatata actctgataa gaataattgc
     3601 tgctctgtcc atcttcggta attgaaattg ttcgcgacaa aagtaccgta ctggcattag
     3661 gccgtagtct caccggttat cctggaagta ccactttttg gaaaaaggag tcccactttt
     3721 tatgagactt tcccgatata ttcacttggg gacatccctt cgaacttcct gaaagccctg
     3781 taaaatgaag cttttgagtt aaatccgcag ttataggcga tgcccattat ggagagatgg
     3841 ctgtttgcct ttaaaacgca ctgctcttta aattcaccta ccctgtacct gttgatgaaa
     3901 tcgaaaaaat tctggttaag ccgggcgttg agcacctctg aaaggaactc gactttgaca
     3961 ttcagcatct cgctcagcct ggagattgta atatccggat caaggtatgg ctgcttctcc
     4021 tccatatccc gggtgagagt ttttataaag gcatcgtctg tgtttttgac gggagatgaa
     4081 gcctcagtta aagctgattt aacgcctgat ttctgtattt ctttcgattg gtttataaag
     4141 atattaccct gccgtatccc gaagtaaccc aggaaaagga tgtataccga tgcaaaacca
     4201 ttcgttaaga ttatcaggaa attgtaggtc gcgaaatgga agatcatgtc aaggttgaag
     4261 agggtcacgt tgactgagta gcttatcagc gtggcaattg tgagtgcgct gagccaaccg
     4321 aggtcgatgt cttcaatttt cgagtaatgc tgcattaatc tgtcccgcct tctcctgatc
     4381 aggttgagag cccatatatt gtaactgatg gtggacacac caatcagcat aactgttatc
     4441 ttataagaga ccgattcctt gaacaggtca ttttcaagga acctgatctt ttcaggaaca
     4501 gtgagattga tgaaattgaa atagtggaag accgaaaaga agatgaaagg tacgaagtga
     4561 agcaggtaca ggggtttgaa tctgaattcg tgatcggata agctcctgat gtaaaaccac
     4621 agcgccggac catgaaggaa gaggaatacc cagcttgtac tgattattgc ggaatatgtg
     4681 aaattgtggc tgaagttttg taattcgaga taaacgcccc cgatgctgat agcgtataac
     4741 acgaagattg ccgcaagaaa ataatccgga agtgatttgt tctttttccc gagcagcagc
     4801 agaagcagaa acagggcttc gaaagttcct ataataagga tgtattccat ttcttcctgg
     4861 ccttgatttc catctggaga atgcagttgt agacaatcct gttgacaggg attttcacgc
     4921 catttgaaaa tgtcttacaa agttacctga cccagttgac aatctcctct tttgacttgc
     4981 ggggagatgc accggtgacg gcgtcagtgc ctttctctat gttacccaca cggagcatta
     5041 tgacaggggt gaatccttcc ggtatctgca gtgcctcccg tttttccctg gctgcggcaa
     5101 tgggaccgcc ataaatcctt gccccgaggc ccatggaggt ggcagccacg aacatgttct
     5161 gtgttgccag cccgcagtcg aagtcgggtg tggagcccga ctgtgacact ttgcctgaga
     5221 tgattatgat cacattgccg ggtgttatat tggagatgat ctcctttatc attgcttcat
     5281 tcttaagcac cgtgaagtgc catggttgtc cgttgcgcga gctgggcgcc ttgatcccgc
     5341 actgcagaat catatcaatc tgctggtcgg taacggccac ctcggtgtaa ttgcgtggcg
     5401 accatgcaga gaggaggaga tcagtgacag aattgcctgc cgtttgtgac atggcgctgc
     5461 cggtggcaag gacgagaagt ccgaatacta atgataatct tctggttttc atgacttgag
     5521 ggctttttat ttgttccata aacccatggc tgaagatagt caaaaaaata ggctgcaaga
     5581 atacgtattc aggcagctgt tcttcttgaa gagggagatt ggagtgaaaa tgcacctctt
     5641 cggaattacc tgaccatgcc gctaaatctt cctccctatg taaaacacat agccgtaata
     5701 ctccttgtac ttgtcatata gctgaacttc atgcctttgg aaagcaacaa gatcttcggc
     5761 agctttattc cccggatact ttttcaggaa tgcttcctgt atcgaagcct ggggttcatg
     5821 gtaattaacc gtccagcact cttccggcag gataaaagca gcaaccggaa catacccggc
     5881 tttctgcatc tggtcaacct tcctcggtat tgtgtcaatc tcagcatatg catccatcca
     5941 gaacctgttt atctcttccg gccgttcttc ggtgaaccat gaactctcgg aaactgcaat
     6001 atatcctccc tttttcagaa attttctcca ttccttcagg ccccgctcaa acccgatatt
     6061 atagattgcc ccctcagacc agaccagatc cagctcctca tccccgaaag gaagtttgtc
     6121 catcgaacct gtaatccctt taaccctgtc ctgaagattc agtttcccgg cattggcatt
     6181 aaacagctcg atgaatttcg ggaacagatc gatccctgta atccttcccc gtgcattctg
     6241 cgccagaacc atcgtctgcc cgcctgtgcc acagccgata tcggcaatca ccgagtcatt
     6301 cgtgagattg tcgataaaac tcaaagcctt tttagtcacc tccggactgc cgggcccctg
     6361 tctttcaaaa tttgagaaat actcacagat cagaccgtag tcgatttcgt ggattgactg
     6421 attttcactg ctcatgatta ttgaatttgt taagcagcaa gttatgaaac ttccggaaaa
     6481 tgccgcgatt gtcatcccgg aaaaatgaag acgaatgtct ctcactgagg catttcagcc
     6541 attggccggc ttaaaatggc tcttcaattg aaaataattt ttctgcactt gtttacatgg
     6601 cgtaactttg ttcatctgac agggggaacc aattagtata cagacctata agaagtacca
     6661 gattatgaaa gttacagcat ttatcggaag cggaagaaag cggcattcat acaatgcctg
     6721 cgaaaaattc ctgcagaacc tgcagacgat gggcaatgtg gagtatgaga tggttcggtt
     6781 aagcgagtac aacatcggaa cctgcagggg ttgcaggatc tgcttcgaca agggggagga
     6841 attctgtccc cataaggatg accgggataa gttgattgaa aagatgaagg attctgacgg
     6901 ggtgattttt gcatcaccca actattcctt tcaggtctcg ggtatgatga aggttttcct
//
LOCUS       DAOPXU010000128         6860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_13493_length_6860_cov_11.945481, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000128 DAOPXU010000000
VERSION     DAOPXU010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13493_length_6860_cov_11.945481"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PKK03_10720"
     CDS             <1..750
                     /locus_tag="PKK03_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84911.1"
                     /translation="NRGTVDNPLGMFSVGGLTLEPGQKVKARIEVEGFTVESEWIETD
                     GLMVSAVEHENFRASARPGSETFSADSSFVVVSAIRGETAPEGTVTLVRGADAPHASL
                     TAPQSVPEFPEAKKAKVWFRKEVRLVPLAEHPGAAIASTGPWNVAYGFSSPGDALDPL
                     KNRMHPFEMVTYVFKGKNLGYSVVTDECISCTAPANVIDRIGRESIPGRPSDTTGRPA
                     TVHNEPGGTPAVKPGQQVRKPVVIRQSSPTY"
     gene            complement(740..2065)
                     /locus_tag="PKK03_10725"
     CDS             complement(740..2065)
                     /locus_tag="PKK03_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HNX84912.1"
                     /translation="MKYNRPLRRMLELINLESDLDEYENIHDTIEKELVFKGTNLWIL
                     VFAILVASVGLNMNSTAVIIGAMLISPLMGPINGIGYSIATYDLSLFRQALKNFTFAV
                     IAGLLASTAYFAISPISTAHSELLARTSPTIYDVLIAFFGGMAGIVAITSRKKGNVIP
                     GVAIATALMPPLCTAGYGLATGHFTFFFGALYLFTINTVFIALSAFLISQILKLPIRG
                     TIADAQKKRINQMISVVLTLVLIPSIYFGYLMVQKEKFTERAGMYVENISVVEGNYLL
                     KHEINPKTRTIQLIYGGTSLTEAQKEDILERANDFSLGDVNVQVEQGFSYADLALRNS
                     EVETLKAEINRLNLLLRERNAELTGISAIREKGSDLLAEMAVIYPQVSEVSYARTLNY
                     VKERETPDTLALVIVTTKGKDLTVKEKEQVEKWLSTRLKSGNVKVLFNK"
     gene            complement(2243..3964)
                     /locus_tag="PKK03_10730"
     CDS             complement(2243..3964)
                     /locus_tag="PKK03_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNX84913.1"
                     /translation="MTLALMALAVMLARAQEVKVPLRFDFYYSYEKMVEAMKALNTAY
                     PEFTKLDEVGRSEEGRIIWALTINNPKTGEPFSKPGVYVDGNIHGNEIQAGEVCLCLA
                     NRLLTNYGKITEITDVVDRNVFYIIPVVNVDGRAHFFSDANTPSSNRGLRIRMDDDRD
                     GLYDEDGPDDLDGDGNITNMRIRDTLGRLRSDPSDKRLMITVKPGEKGEWTRLGQEGI
                     DNDGDGQVNEDGEGYVDGNRNWGMNWNPPYVQSGAGNYPFEGKGIRAIAEWMLARPNI
                     ILVFAFHNNGGMYLRGPTSKAAGELPQGDVAVYDYLGGHMEKIVPGYQYLISWKDLYS
                     TYGDFTDFTDKLVGSYSLVGELYQPETETYDGTMKRGEEERSRSEDPSAGERQRLLFD
                     DNVNQGELFKEWKAYKHPLYGDIEIGGWTKMSSRLPHPFMLQDLVHRNASSVIFAARQ
                     TPKITMDVFEVKKVGGDLWSVRVRLVNSGALPSVTYETMQNKLYPIDKLTVTGRSAKV
                     AGGGVLTNAWMNLVTYKEYRPEVQMCQVPGLGKVEYQFLVSGRGDIEIKYESRKAGTI
                     SKTAELK"
     gene            complement(3990..5837)
                     /locus_tag="PKK03_10735"
     CDS             complement(3990..5837)
                     /locus_tag="PKK03_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNX84914.1"
                     /translation="MNYTRLHVMGGMMLLVALLLMAPAPVRATEKYSDPAAVNRMVEQ
                     LRLANPSLVKVHKLAVTPGGREMLMIEIGKSGTSVPAVLVGANFSGLTPLATEAAMSL
                     ASRVVSDASLNGKLTWYILPVGNPDAYARYFTKPLWADPGNGRPFNDDTDDQTDEDGY
                     NDLDGNGIITQMRVKVPDGTWVPVEGEPRLMRKADAAKGERGIYKLYTEGIDDDGDGQ
                     YNEDVPGGTNVNINFPHLFKKFGRRSGLYPGSEPESEAMLKFAFSHPEIAMVLAFGQT
                     NYLVSPPKGGKKGPVDTENIRIPREIGASLGIDVSRTYTMAEVIELVQPMLPPGMTAD
                     ERMIASFLGLGAVVNPMPDDMTFYNEISSDYKEYLKKKGVTAERFDPAQPEDGSFEQW
                     AYYQLGLPVFTMDFWGLPKPKEEKKESQGDEKKNDTVKVNQKKGDPKELAQLAFSDKW
                     LDGHGFIPWKSYRHPTLGEVEIGGFTPFIDNTPPESMIDSLLDLQLPYIPELVKRLPR
                     LAIAEVKTTEKGGGVYQLEAWVTNEGYLSFPIAMGKRNKVPAPAILTLEGDGIEILSG
                     KKRTPVGEVGGMKSVKYVWLVRSPKKGTLTLRLESKQAGNDSETTNIGG"
     gene            complement(5865..>6860)
                     /locus_tag="PKK03_10740"
     CDS             complement(5865..>6860)
                     /locus_tag="PKK03_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84915.1"
                     /translation="GFNSAGFAIMNSAAYNNNSGDTTKLADREGEVMKMALASCATLA
                     DFEKMLSEMPKPIGVDANFGVIDAQGGAAYYETGNFSWKKIDANDPAIAPYGLIIRTN
                     HSFTGTPDIGFGYIRYEAASLALNMALAQKKLDPQNLLNSISRNLSHALTKENLRDNL
                     PASPADARFRYFEDFIPRPSTASVMMVVGAAPGEDPAGAMMWTLAGFPLTTVAVPVWV
                     SAGKNLPAVVSMKENMHAPLCDAAMTLKNQLFPIKRGSGPKYINVAILLNQEKTGILQ
                     KLGPVETEIFMKTAGLVEALPAKGKQQKEAILEHYKWLDGYIIQSYKELFGIEVK"
BASE COUNT         1433 a         1926 c         1782 g         1719 t
ORIGIN      
        1 aaccgtggca cggttgacaa tcctcttgga atgttctcgg tgggcggcct cacacttgaa
       61 ccgggacaga aggtgaaagc ccggatagag gtggaagggt tcacggtgga gtcggagtgg
      121 attgagaccg acggactgat ggtttcagct gttgagcatg aaaatttcag ggcatcagcc
      181 aggcccggaa gcgagacctt ctcggcagac agcagcttcg tggtggtgag tgccatccgc
      241 ggtgagactg ctccggaagg aaccgttacg ctggtgcgcg gagccgatgc accacatgcg
      301 tcactgacag caccccagtc cgtacccgag ttccccgagg caaaaaaggc aaaggtatgg
      361 ttcagaaaag aggtcaggct ggttcctctt gcggaacacc cgggtgctgc catagcatca
      421 accggacctt ggaacgtggc atacggattc tcatcaccgg gagatgccct ggaccccctc
      481 aaaaacagga tgcacccgtt tgagatggtg acttatgtct tcaagggaaa gaacctcggg
      541 tactcagtcg tcacggatga atgtatatca tgcaccgctc cggctaatgt cattgacagg
      601 attggcaggg agagtattcc cggaaggccg tcagacacaa cgggtcgtcc tgcgacggtt
      661 cacaatgaac ccggtggcac gccggcggtc aagcccggcc agcaggtaag aaagccggtg
      721 gttataagac agagcagtcc tacttattga acaggacctt cacgttaccg gattttagcc
      781 tggttgagag ccatttctca acctgctctt tttccttgac ggtgaggtct ttacccttgg
      841 tcgtcactat gaccagcgcc agtgtgtcag gagtttccct ctccttcaca tagttgagcg
      901 tccgcgcata ggagacctct gatacctggg gataaattac cgccatctcg gcaaggaggt
      961 cacttccctt ttcccttatg gctgagattc cggtcagctc ggcattcctc tcccggagaa
     1021 gcaggttcag acggttgatc tctgccttca gcgtctccac ttcactgttt cgtaatgcga
     1081 gatcggcata ggagaatccc tgttccacct ggacattcac atccccgaga gagaagtcgt
     1141 tggcccgttc gaggatatcc tccttctgcg cctcggtgag ggatgtgcct ccgtagataa
     1201 gctgaattgt gcgggttttg gggttgatct catgcttgag cagatagttg ccttcaacaa
     1261 cactgatgtt ttcaacatac attccggccc gttcagtgaa tttctccttc tgaaccatca
     1321 ggtatccaaa ataaatactg ggaatcagca ccagtgtcag gacaaccgag atcatctggt
     1381 taatcctctt tttctgcgca tccgcgatgg tgcccctgat aggaagcttg agtatctgtg
     1441 agataagaaa ggccgacagg gcgatgaata ccgtgttgat ggtgaagagg taaagggccc
     1501 cgaagaaaaa ggtgaaatgt cccgtcgcaa ggccgtaccc ggcggtacaa agcggtggca
     1561 tgagagccgt ggcgatcgcc acccccggga tgacgttgcc cttcttcctg ctggtgatgg
     1621 ccacaatgcc ggccattcct ccgaaaaagg cgatcagcac atcatatatg gtagggctgg
     1681 tgcgggcaag caactcggag tgggcggtag agatcgggct gatggcaaaa taggcggtgg
     1741 aggccagaag accggctatc acggcaaagg tgaagttctt cagtgcctgc ctgaagaggg
     1801 agaggtcata ggtggcaatg ctgtatccga tgccgttgat cggacccatc agcggagaga
     1861 tgagcatcgc accaatgatc actgcggtgg aattcatgtt cagcccaacc gatgcaacca
     1921 ggatggcaaa gaccaggatc cagaggttgg tgcccttgaa aacgagctcc ttctctatgg
     1981 tgtcatggat attttcatat tcatcgaggt cactctcaag gtttatcagc tcgagcattc
     2041 tcctgagcgg tctgttgtat ttcataatct ctgtttgctg atacaaatat agttatttgg
     2101 ggtattctgg tcaggcaaca gtccagtcag cagtctgaga ctgcagcggc agcgcaggat
     2161 cagtcccgta tccgccaccg gcggatcggg aaggcagtat tagtaagcag ccggcagtct
     2221 gagactgcac cgccgggcgg aatcacttca gttccgccgt cttgctgatg gtgcccgcct
     2281 tgcgcgactc gtacttaatc tcaatatcac ccctgccgct gaccaggaac tggtactcca
     2341 cctttccgag acccggcacc tggcacattt gtacctcggg acggtactcc ttgtaagtga
     2401 caagattcat ccatgcgttg gtcagcaccc caccgccggc cactttggca ctccgacccg
     2461 taacagtcaa cttatcaatt ggatacaact tattctgcat cgtctcgtat gttactgacg
     2521 gcagggcgcc gctgttcacc agcctaaccc ttacactcca caggtcaccg ccaacctttt
     2581 tcacctcaaa cacatccatc gtaattttcg gtgtctgcct ggcagcgaag atgactgaag
     2641 aggcgttcct gtgcaccagg tcctgcagca tgaacggatg cggcagacgc gaactcattt
     2701 tcgtccaccc tccaatctct atgtcaccgt aaagcggatg cttataagcc ttccactcct
     2761 taaagagctc accctggtta acgttgtcat cgaacaacag tctctgccgc tcacctgctg
     2821 agggatcttc acttcttgaa cgctcttcct cacccctctt catcgtgccg tcataggtct
     2881 cagtctcagg ttggtacagc tctcccacca gtgagtatga ccctacaagc ttgtctgtaa
     2941 aatcagtaaa gtcgccgtag gtagaataga ggtccttcca gctgatcagg tactggtatc
     3001 cgggcacgat cttctccatg tgccctccga ggtagtcata tactgccacg tctccctgag
     3061 gaagctcgcc ggcagccttt gaggtggggc ccctgagata catgcctccg ttattgtgga
     3121 aggcaaacac caggatgata ttgggccttg ccagcatcca ttcggcgatg gccctgatgc
     3181 ccttcccctc gaaggggtag tttcccgctc cgctctgcac gtaaggagga ttccagttca
     3241 ttccccagtt gcggtttccg tcgacatacc cttcgccatc ctcattgacc tgtccgtcgc
     3301 cgtcattgtc tatcccttcc tgcccgagcc gtgtccattc acccttctca ccgggcttga
     3361 cggtgatcat cagccgcttg tcggaaggat cgctcctcag cctgccgagg gtgtccctta
     3421 ttctcatgtt ggtgatattt ccgtcgccgt cgaggtcatc cggaccgtcc tcatcataca
     3481 atccgtcgcg atcatcatcc atccgtatcc gcagtcccct gttagagctg ggagtatttg
     3541 catccgagaa gaaatgcgcc ctgccgtcga cattcaccac cggtattata tagaacacgt
     3601 tacggtctac aacatcagtt atctcagtaa ttttcccgta gtttgtcagc agcctgttgg
     3661 ccaggcaaag acaaacctcc cctgcctgga tctcgttgcc gtggatgttg ccgtcgacat
     3721 acactcccgg cttggaaaat ggctctccgg tcttcgggtt attgattgtc agcgcccaga
     3781 ttatccttcc ttcctcgctt cgtcccacct catcaagctt tgtgaattcc ggatatgctg
     3841 tgttgagcgc cttcattgcc tcaaccatct tttcataact gtaatagaaa tcaaagcgga
     3901 ggggcacctt cacctcctgc gcccgggcta gcatgacggc aagcgccatc aatgccaatg
     3961 tcataaatac tctttttctt tccatggctc taccctccga tgtttgtcgt ttcactgtca
     4021 ttacctgcct gtttcgactc cagcctgagt gtcaatgtcc ctttcttcgg gctcctgacg
     4081 agccagacat atttgactga tttcatgcct cccacctcgc cgacgggagt ccttttcttg
     4141 ccggaaagga tttcaatccc atctccttcg agggtcagta tggcgggtgc cggcaccttg
     4201 ttccgcttgc ccatagctat agggaaggag aggtaaccct cattggtcac ccatgcctca
     4261 agctggtaaa ctccgcctcc cttttccgtt gtcttaacct cagcgatagc aagccgtggc
     4321 agtcttttca ccagttccgg gatgtaagga agctgcaggt ccaacagtga gtcaatcatg
     4381 ctttccgggg gtgtgttgtc aatgaacgga gtaaacccgc ctatctccac ctcgccaagg
     4441 gtcggatgcc tgtagctctt ccaggggatg aatccatggc catcgagcca tttgtcggag
     4501 aaagccagct gtgccaactc cttcggatcg cccttcttct ggtttacctt cactgtgtcg
     4561 ttcttctttt catcgccctg gctttccttc ttttcctcct tcggtttcgg aaggccccag
     4621 aaatccatgg tgaacaccgg cagaccgagc tggtagtatg cccactgctc gaatgatccg
     4681 tcttccggct gggccggatc gaacctttcg gcggtgacgc ccttcttctt caggtactcc
     4741 ttgtagtctg acgagatctc attatagaat gtcatgtcat caggcatcgg gttaaccacg
     4801 gccccaaggc caagaaagct ggctatcatt ctctcgtcag ccgtcatgcc tgggggcagc
     4861 atcggctgca ccagttcaat gacctcggcc atggtatagg ttcgtgagac gtcaataccg
     4921 agtgaggcgc ctatctcccg tggtatgcgg atgttttcgg tatcaaccgg gccctttttg
     4981 ccacccttcg ggggcgaaac gaggtagttg gtctggccga aggcaagaac catggcaatt
     5041 tctggatgcg agaacgcgaa cttaagcatc gcctccgact caggctccga accgggataa
     5101 aggccgctgc gcctgccgaa ttttttaaac aggtgcggga agttgatgtt gacgttcgtg
     5161 ccgccaggca catcctcgtt atactgaccg tcaccgtcgt catcgatgcc ttcagtgtag
     5221 agcttataga ttcccctctc gccctttgcg gcatcagcct ttcgcatcag gcgcggttcg
     5281 ccctcaacgg gtacccatgt gccgtcaggc accttcaccc tcatctgcgt tatgatgccg
     5341 ttgccgtcga ggtcattgta gccgtcctca tcagtctggt catcagtatc atcgttgaac
     5401 ggcctgccgt tgccgggatc ggcccagagc ggtttggtga agtaacgggc ataggcatca
     5461 ggattgccca ctggcaatat gtaccatgtc agcttcccgt tcagcgacgc gtcggagaca
     5521 acccgcgagg ctagtgacat tgcagcctcc gtagcaagcg gagtaaggcc tgaaaaattg
     5581 gcaccgacga gcaccgccgg aacggaagtg cctgactttc caatctcaat catcagcatc
     5641 tccctgccgc cgggggtgac tgcgagctta tgcaccttca ccagcgaagg attcgccagt
     5701 cggagctgct ctaccatgcg gttcactgct gccggatcac tgtacttttc ggttgctctg
     5761 accggtgccg gcgccatcag cagcaaagcg acaagaagca tcatgcctcc catcacgtgt
     5821 aatctggtat aattcatcct ggaaatggtt ttgcgtggta tctgctactt cacttcgatt
     5881 ccaaataact ctttgtatga ctgtattata tagccgtcga gccatttata gtgttcgagg
     5941 atagcctctt tctgttgctt tccctttgca ggtaatgcct caaccaggcc ggcagttttc
     6001 atgaagatct ctgtttccac cggtccgagc ttctgcagaa taccggtttt ttcctgattg
     6061 aggagtatgg ctacatttat gtacttcgga ccggagcccc ttttaatggg gaagagctgg
     6121 ttcttgaggg tcatggccgc atcacagagg ggagcatgca tattttcctt catgctcacc
     6181 acagccggca ggttctttcc tgcgctgacc cacaccggta ctgccaccgt cgtcagcggg
     6241 aagcctgcaa gtgtccacat catggcaccg gccgggtctt cacccggggc tgcgccgaca
     6301 accatcatca cggatgctgt tgaaggcctt ggaatgaagt cctcaaaata cctgaacctg
     6361 gcatctgcag ggcttgcagg aagattgtcg cgaaggtttt ccttcgtcag ggcgtgtgac
     6421 aggttgcgcg atatgctgtt gagcaggttc tgcggatcga gctttttctg tgcgagcgcc
     6481 atgttcaggg cgaggcttgc agcctcatac ctgatatagc cgaaacctat gtccggagtg
     6541 ccggtgaatg agtggttggt gcgtattata agaccgtaag gtgcgattgc cgggtcattg
     6601 gcatcaatct ttttccagct gaagttgccg gtttcataat aggctgcacc tccctgtgcg
     6661 tcaattactc cgaagttggc atcaactccg attggttttg gcatttcgct gagcatcttc
     6721 tcgaaatcgg ccagggtggc gcaggaggca agagccatct tcatcacttc gccctcgcgg
     6781 tctgccagct tggtggtatc accgctgttg ttgttatagg cagctgagtt catgatggcg
     6841 aagcctgcgg agttgaatcc
//
LOCUS       DAOPXU010000129         6807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_13632_length_6807_cov_11.504591, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000129 DAOPXU010000000
VERSION     DAOPXU010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13632_length_6807_cov_11.504591"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /locus_tag="PKK03_10745"
     CDS             complement(<1..145)
                     /locus_tag="PKK03_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HNX84916.1"
                     /translation="MEKELRLMKGNEAIAEAAIRAGADGYFGYPITPQSEILEYLMEA
                     NPQA"
     gene            complement(167..394)
                     /locus_tag="PKK03_10750"
     CDS             complement(167..394)
                     /locus_tag="PKK03_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX84917.1"
                     /translation="MAKIKGAVVVDREKCKGCEVCVVTCPTQTLALATEVNSKGYHFS
                     YAANPETCIGCSNCAVVCPDGVITVYKVKIG"
     gene            complement(431..1753)
                     /locus_tag="PKK03_10755"
     CDS             complement(431..1753)
                     /locus_tag="PKK03_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNX84918.1"
                     /translation="MKNTPINRQTVSNAIVSSGITDLGRASIREIVKLVNVLRRETGV
                     EFIRMEMGVPGLPSAEVGVRAEIEALRRGVASDYPDIEGLPEMKTEASKFVKNFLDVS
                     VKPECCVPTGGAMMGGMASFMVANRNDHTKEGTLFIDPGFPVQKTQTRILGQDFRSFD
                     VYNYRGEKLGPKLESILECGKVSTLLYSNPNNPSWICFTDDELRTIGTLARKYDVIVI
                     EDLAYFGMDFREDYSVPGQPPFQPTVAKYCDDYILLISSSKIFSYAGQRIGLLVMSDH
                     LYNRRYTDLLRYYSTDIFGRAMIFGALYALSSGVSHSAQYGFTAILKAVNEGLYNFVD
                     NTRVYGEKAAKMKEMFTRNGFSIVYDMDIDRPVADGFYFTISYPGLSGGELLQELLSY
                     GIAAITLNICGSERHEGLRACVSQVRKSQLPLLEERLTLFSENNPIGK"
     gene            complement(1899..2594)
                     /locus_tag="PKK03_10760"
     CDS             complement(1899..2594)
                     /locus_tag="PKK03_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84919.1"
                     /translation="MKRSDIVFVIVFAGLFIPFFLSRRLYDFFVWFSTDWAFTASFIK
                     FAILATMGEMLGSRIRTGIYLPAGFALLYRAVVWGFLGMTVKAAFMIFGGGTPLILEH
                     LGMENASAVMAGPVTGARVLLAFAISTFLNIFYAPVLMVTHRVSDLHVAAAAGDRRKL
                     LTVPDVKGLLASIDWNSMWGIVLKKSIPLFWIPAQTVNFLLPEEFRMLVAALYSTVLG
                     VILALAAGAGKRI"
     gene            complement(2691..4820)
                     /locus_tag="PKK03_10765"
     CDS             complement(2691..4820)
                     /locus_tag="PKK03_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNX84920.1"
                     /translation="MINAKLLNPKSIVVVGGSDDVTKAGGKVLKNLLTGDFPGSIYVV
                     NPKATVVQGLTAFADVSLLPRTDLAVIAVAARYCPGIVRTLATEKGTGGFIILSAGFS
                     EENEEGAGFEKEIVEIINGAGGTLIGPNCIGYLNKNYHGVFTEPIPRLSQQGIDFISG
                     SGATAVFILETAITGGLPFSSVWSVGNSAQTGVEDVLEHLDLTFDPLTSSRIKLLYIE
                     NIADPQRFLRHSSSLIRKGCRIAAIKAGSSEAGSRAASSHTGAMASPDTAVDALLRKA
                     GIVRCYSRADLAAVAALFTYRPLKGKRIAVVTHAGGPAVMLTDALSEGGMEIPRLAGP
                     AAERLLEKLFPGSSVANPIDFLATGTAEQLGHIIDACENDFEEIDAIAVIFGSPGLFP
                     VDDVYSLLHDKIRSCRKPIYPILPSVINVKREIAEFIAHGNTIFTDEVLFGRALCRVN
                     ATPAPAVTEKIEDVRAGSSDLNGNSARTDSRVIREVIDGAGEGYLPPEKVGILLRACG
                     IETVPETVVTSAEAAVRAAASMGYPVVMKVVGPVHKSDIGGVTLNIGDGEKVREEFAR
                     MMALPEVTGMLIQKMISGHELFCGATREGRFGHLVMAGMGGIFIEVFKDFATALVPVS
                     DQEARSMIRSLKSYKIIKGIRGSAGVNEEAFVRVITSLSDLLAAAPEIEEMDINPLLG
                     TPEKIIAVDARIRVGSLLSAAGSHYGK"
     assembly_gap    5108..5207
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5419..6546)
                     /locus_tag="PKK03_10770"
     CDS             complement(5419..6546)
                     /locus_tag="PKK03_10770"
                     /inference="COORDINATES: protein motif:HMM:NF025336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4221 family protein"
                     /protein_id="HNX84921.1"
                     /translation="MRTAGLLFILLLAGCAIGTQEADFENELIIADTIVRFPVSDQTY
                     PQSQAVELFESYDSVRYLCYLNPGKNEILFFFLDSIKLSHKISLAQDGPHGVGKAVGF
                     SVGSLDSVYVTSKGRKILYLVDRNGLLLSSNDYSAASPLSMSSYSTTKTPVFLTDTCL
                     YLPLLPAGNWSTITEKQMVSFNVCLSLNLTRGAASLCPACYPESAFETRSVQYQWCRG
                     DELYVISFERNDKLYATVDFAELKATTAKSNFHRKTESPPANPAPEQYLRYSIETPSY
                     TGIIYDKYRDVYYRICYQGKTTGKDDNLMQLASFKPSYSILILDKHLEVIGETLLPEN
                     QFYPEEYFVAKEGLYISENSVFNENYDENYLAYRLLRLTKR"
     gene            complement(6605..>6807)
                     /locus_tag="PKK03_10775"
     CDS             complement(6605..>6807)
                     /locus_tag="PKK03_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84922.1"
                     /translation="TNLYANMKKKAIVSLLFVLLLTGYFLSRQVRAEKDPTFYCWFNE
                     AIKDCDTGGMWVCYGVYPPDCN"
BASE COUNT         1748 a         1695 c         1491 g         1773 t
ORIGIN      
        1 ttgcctgggg attggcctcc ataagatatt caagtatctc gctctgcgga gtgatgggat
       61 acccgaaata accgtcggca ccggcgcgta tcgccgcttc ggcgatagct tcgttgccct
      121 tcatcagtct cagttccttt tccattaact tatattgttt ctgtcgttat ccgattttta
      181 ccttgtagac ggtgatcacg ccgtcaggac aaacaaccgc gcagtttgag catccgatgc
      241 aggtctccgg gttggctgca tagctgaaat gatatccctt tgaattcact tccgttgcca
      301 gggcaagcgt ctgggtggga catgtaacca cgcacacctc gcaacccttg cacttctcgc
      361 ggtcaacaac aactgctcct tttatctttg ccatatcttt attgagttaa cttcggttca
      421 aaagtattat ttatttccct atcgggttat tttcactgaa aagtgtcagt ctttcttcaa
      481 ggaggggcag ctgacttttt cgcacctgcg agacacatgc cctgagccct tcatgccgct
      541 cactgccgca gatgttcagt gtgatggcgg ctataccgta tgaaagcaat tcctgcaaaa
      601 gctctcctcc gctcaggcca ggataggaga ttgtaaaata aaatccgtct gccaccggcc
      661 tgtcgatgtc catgtcatat acgatgctga aaccgttcct tgtgaacatc tccttcattt
      721 ttgcagcctt ctcgccgtat accctggtgt tgtcaacaaa attgtaaagt ccttcattga
      781 ccgccttgag tatggctgta aaaccgtact gtgcggagtg gcttacccct gaagaaaggg
      841 cgtacagggc accgaagatc attgccctgc cgaaaatatc tgtagaataa tagcgcagaa
      901 ggtcagtgta gcgacggtta tatagatggt cagacatcac aaggaggccg attctctggc
      961 cggcataact gaatatcttt gagctggata tcagcagaat ataatcatca cagtattttg
     1021 caaccgttgg ctggaaaggc ggctggccgg ggacagagta gtcttcgcgg aaatccatgc
     1081 cgaaataggc caggtcctcg ataactatca catcatactt tctggccagg gtgccgatcg
     1141 ttcgaagctc gtcgtcagtg aaacagatcc acgacgggtt attggggttg gagtaaagga
     1201 gggttgacac ctttccgcac tccaatatcg attccagttt cgggccaagc ttctctccgc
     1261 ggtaattata tacatcaaat gatctgaagt cctgtccgag tatacgtgtc tgggtcttct
     1321 gcaccgggaa tccggggtcg atgaagagtg ttccctcctt tgtatggtca tttcggttgg
     1381 ccaccatgaa gcttgccatc cctcccatca tggccccgcc ggtggggaca cagcattcgg
     1441 gtttcacaga cacatcaagg aaattcttca cgaatttcga ggcttcggtt ttcatttcag
     1501 gaagcccttc gatgtcaggg tagtcggatg cgacgcccct tctcagggct tcaatttctg
     1561 ccctgactcc cacctctgcc gatgggagtc cggggactcc catctccatg cggatgaatt
     1621 ccactccggt ctcgcggcgc agcacattca ccagcttgac tatctcgcgg atgctcgcac
     1681 gacccaggtc agtgatcccg ctggatacta tggcgttgct tactgtttga cgattaatgg
     1741 gggtattttt cataaatttt tcatttagtg ccctcatttg tggctctaag ataaaaattt
     1801 ataagggaac gataactgct atttgtcata tttgaatttg caattgacga tttgcgatgt
     1861 tcgattgctt ttaaatcgca attcgcaaat ctcaaatctc aaatcctctt gcctgctccc
     1921 gcagccagtg caagtatgac tccgaggacg gtgctgtaga gtgccgcaac aagcattctg
     1981 aactcttcgg gcagcagaaa gttgactgtc tgtgccggta tccagaacag gggtatgctc
     2041 tttttcagca caatacccca catactgttc caatcgattg atgccaggag ccctttcaca
     2101 tcgggtactg tcaaaagctt ccgacggtca cctgcagctg cggcaacgtg caggtcactg
     2161 acacgatggg ttaccatcag aaccggggcg taaaaaatat tcaggaaggt actgatcgca
     2221 aatgcgagca gcaccctggc tccggtcacc ggaccagcca ttactgctga tgcattctcc
     2281 atccccaggt gttccaatat aagtggtgtg ccgccgccga agatcataaa agcagccttg
     2341 actgtcatcc ccaggaatcc ccatacaaca gccctgtaca atagggcaaa acctgccggc
     2401 aggtaaatcc cggtacggat cctgctgcca agcatctcac ccattgttgc gaggattgca
     2461 aattttatga aacttgcagt aaatgcccag tcagtgctga accagacaaa aaaatcatag
     2521 agccggcgcg acaggaaaaa cggaataaat aatccggcaa agacgataac aaaaactata
     2581 tctgaacgct tcatgtggat ttgcgaattg cgatgtgcga tgtgcgattc agggttttgg
     2641 acatgctgtt ctacaattct aataggtcta cgaccaattt ccatgtgatc ctattttccg
     2701 taatgactgc cggctgccga aaggagactt cctactctta tcctggcgtc aacagcaata
     2761 atcttttcag gcgttcccag cagcggattg atatccatct cctcaatttc aggggcggca
     2821 gccagaagat cagagaggga ggtgatcacc ctgacaaatg cctcctcatt gacgccggcg
     2881 gagccacgga tccccttgat tatcttatat gatttcaatg accgtatcat tgaccgtgcc
     2941 tcctggtcag agacaggcac cagcgcagta gcaaaatctt tgaagacctc gataaagatc
     3001 cctcccatgc ccgccatcac caggtggccg aaacggccct cgcgggttgc gccgcagaat
     3061 agctcgtgac cgcttatcat cttctgtatc agcataccgg tcacctcggg tagcgccatc
     3121 atcctggcaa attcctccct gaccttctcc ccgtcaccaa tattcaaggt gacaccgcca
     3181 atatcagatt tatgcaccgg acccaccacc ttcatcacta ccggataacc cattgatgct
     3241 gcagccctga cggccgcttc ggccgatgta acaacggttt cgggaaccgt ctcaatgcca
     3301 caggccctaa ggaggattcc gaccttttcg ggaggcaggt atccttctcc ggcaccatca
     3361 ataacctccc ttatcaccct gctgtcagtt cgtgcagagt ttccattcag atcagatgat
     3421 ccggcccgga catcctcaat tttctccgtg accgcgggag caggggttgc gttcaccctg
     3481 cagagtgccc tcccgaagag cacctcatca gtgaatatcg tattcccatg tgctataaat
     3541 tcggcgatct cccttttgac atttataaca gaaggcagta tcgggtatat cggcttacgg
     3601 caactgcgta tcttatcatg caggagactg tagacatcat ctacaggaaa gagtccgggg
     3661 ctgccgaata tgacagcgat ggcatcgatc tcttcaaaat cattttcaca tgcatcaatt
     3721 atatgtccga gttgttcggc agtgccggtt gccaggaaat cgatcgggtt tgccaccgat
     3781 gacccgggaa agagtttttc aagcagtcgc tctgctgccg gtcctgccag gcgggggatc
     3841 tccatcccgc cctcactgag ggcatcggta agcatcactg cgggtccacc ggcatgggta
     3901 acgacagcta tcctttttcc tttcaggggt ctgtatgtaa acagtgctgc tacggcagcc
     3961 agatcggcac gactgtaaca cctgacgatt ccggccttac gcagcagggc atccactgcc
     4021 gtatcaggtg aagccatcgc acctgtgtgt gatgatgcag cccttgaccc ggcctctgat
     4081 gaacctgcct tgatggcagc aatcctgcac cccttcctga tcagtgatga tgagtgtctg
     4141 aggaaccgtt gcggatcggc aatgttctca atataaagaa gtttgattct tgagctggtc
     4201 agcggatcaa acgtaagatc tagatgttcc agcacatcct ccacgccggt ctgcgcactg
     4261 ttgccaactg accagacgct ggagaacggc agtcctccag taatagccgt ctcaagaatg
     4321 aagacggctg ttgcccccga accggagatg aagtcaatac cctgttgtga gagccttggt
     4381 atgggctctg tgaatacccc atggtagttt ttgttcaggt agcctatgca gttgggacct
     4441 atgagtgtac ctccggctcc gtttataatt tccacaatct ccttttcgaa gccagccccc
     4501 tcctcattct cctcgctgaa cccggcagaa agaatgatga aacctcccgt gcccttctcc
     4561 gttgccagag tcctgactat gcccgggcag tagcgggcag caactgcaat tactgccagg
     4621 tcagtacggg gaagcagaga cacatcggca aacgctgtca atccctgtac tacagtcgct
     4681 ttagggttta caacataaat gctgcccggg aaatcgccag tcagcaggtt ctttagcacc
     4741 ttgccgccag ccttggtgac gtcatctgat ccgccaacaa cgactatact tttaggattc
     4801 aataatttag cattgatcat aacgggggtc tctgttgcgg gaaaggtaga aacattttga
     4861 aaaaaaacaa atgacagcac taccgtctag attgactcaa cccctagaac tggtcattga
     4921 aagattatct ctgtactact ccggacagtt aaccgcaacg attttatgag tgaacaatct
     4981 gtcaccaggg gccataaaat gacactatgc ttccagatca agggaatgta tgtattaccc
     5041 ctgtatttca ctttaagcta attttaatca atagtatgca tttgtcttta tttttgacaa
     5101 aatcattnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntta ttaacaaaat
     5221 caagtacagg ctgaagacaa ctgctgcacc cagttaaagg aattagaatc agatatgatt
     5281 catcatggca gaattcaaga tcaaatttct tctgaatctg tttgtactcc ttttgaaagt
     5341 tattcttaac ttccggattc tggcaatacg ctaacagaag cagaattaca aaagttactt
     5401 tgaacaattc taatattctc atcgctttgt aagccgtaat aacctgtaag ccagataatt
     5461 ttcatcataa ttctcattga aaactgaatt ttctgaaatg tacaaacctt cttttgcaac
     5521 aaaatactct tccggataga actggttttc aggcaacaga gtttcgccaa tgacttccag
     5581 gtgtttgtca agaataagaa ttgaataaga aggtttgaat gaagccagtt gcatcaggtt
     5641 gtcatccttc ccggtagttt ttccctgata gcaaattctg taatagacat ctctgtattt
     5701 atcataaata atcccggtat aagaaggtgt ctcaattgaa taccttaggt attgttccgg
     5761 agcgggattt gccggaggtg actccgtctt cctgtgaaaa ttacttttag ctgtggttgc
     5821 cttcaattcg gcgaaatcaa ctgtagcata gagcttatca tttctttcaa aagaaataac
     5881 atacaattca tcacctctac accattgata ttgcacactc ctggtctcaa atgctgattc
     5941 agggtagcat gcaggacaaa gggaagcagc acctcttgta aggtttagac taaggcaaac
     6001 attaaacgaa accatttgtt tctcggtaat tgtgctccaa ttgccagcag ggagaagcgg
     6061 caggtaaaga caagtgtcgg taaggaaaac aggcgtttta gttgtcgaat aggatgacat
     6121 gctaagaggg cttgctgccg agtaatcatt gctggaaagc agcaaaccat tcctgtcaac
     6181 caagtatagg atttttcttc cctttgaagt aacatatacc gagtcaagag agcctacgga
     6241 gaatccgacg gctttaccca cgccgtgtgg tccatcctgg gcaagggata ttttgtggct
     6301 aagttttatt gaatccagaa agaagaaaag tatttcattc ttacccgggt tgagataaca
     6361 cagataacgc acggaatcat aagactcaaa taattcaact gcctgagact gtggataagt
     6421 ctggtcagaa accggaaatc ttactattgt atctgcaatg ataagctcat tctcaaaatc
     6481 agcttcttga gtacctatgg cacaacctgc caagagtaaa ataaaaagta aacctgcagt
     6541 tctcatatcc ttttcaacac ataaagctac agccgggccc tggctgtaac attaaaatct
     6601 catttcaatt gcaatccgga ggatataccc cataacagac ccacatacca ccagtatcac
     6661 aatctttgat cgcttcatta aaccaacaat aaaaagttgg atctttctct gccctaacct
     6721 gtcttgataa aaaataacct gtcaggagca atacaaatag cagactaaca atcgctttct
     6781 ttttcatatt cgcatataga ttagtta
//
LOCUS       DAOPXU010000130         6791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_13670_length_6791_cov_13.321853, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000130 DAOPXU010000000
VERSION     DAOPXU010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13670_length_6791_cov_13.321853"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..1306)
                     /locus_tag="PKK03_10780"
     CDS             complement(236..1306)
                     /locus_tag="PKK03_10780"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNX84923.1"
                     /translation="MTVEEFSDTREQEKYTEPDQEGQEQELYEHYRYVVDPGQSMLRI
                     DKYLSKRIENVSRTRVQAAAQAGNILVNDSPVKPNYRVKPLDVIQVLLPNPPREIELI
                     PQDIPVTVVYEDDDLIVVDKAAGMVVHPAYGNYSGTLMNALMYHFRDLPLFQTGEIRP
                     GLVHRIDKNTSGLLVVAKNEYAHNRLARQFFRRTTGRLYTALVWGTPDPSEGTITGHV
                     GRNIRDRKIMQVFPDGSQGKHAVTHYRIIEPLGYVSLIECRLETGRTHQIRVHMAWKG
                     HPLFNDEEYGGHRILKGTTFTKYQQFVRNCFDLLPRQALHARTLSFDHPVTGKRLSFE
                     SPVPQDMVVVIEKWRNYIAGRE"
     gene            complement(1315..3171)
                     /locus_tag="PKK03_10785"
     CDS             complement(1315..3171)
                     /locus_tag="PKK03_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84924.1"
                     /translation="MKNSRAIFLLLAAVLTLDVSGQEVITGLIRNRQVSDAFRPASVL
                     KGATAEEPVPLPFSDDFTGDSIFPSVTRWADLQAFINNTYSVRQPSAGIATLDCLDER
                     GLLHEGASQAVFPADRLTSRTIDLEYQPSDSIFLSFLFEAGGVADLPEAGDSLTLSFW
                     APEEQKWYSIWQATGEATNGFNMVIIPVTDTRYLMKGFRFMFTGHASLAGVISEPSQA
                     GNADHWNIDHVLLDKGRSVHDTVLRDVALTLPPRSLVKEYEAMPWRHFRQAYLSAMSP
                     VAPVKYRNNDTIVRNVTRHVTITDMSDNTVVRDFDAGAANAQPLSDVSYNAPLLYTYN
                     TTASADTAQFMVTISLITDDFDPKQNDTLRYIQKFSDYFAFDDGTAEAGYGINGQGSN
                     NAMAALRFRSFLPDSVTGISFCFNDAYDNANQRAFDIMVWADVNGRPGALLGSSDGPV
                     ASPAGEINGFVTYMFDTPVRVTDNFWIGWRQNSEKFLNAGLDLNTAPAGRQYFHLSGI
                     WQESQAPGIIMMRPVMKGSGIPNSSDNGTLINNLFSLYPNPTDGLVTLVPAEGAPDDF
                     TIDVISSSGAVVMSPGNPKRPDLSRLAAGNYMLLIRTRDGRPLSLLRVVLVK"
     gene            complement(3174..4031)
                     /locus_tag="PKK03_10790"
     CDS             complement(3174..4031)
                     /locus_tag="PKK03_10790"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNX84925.1"
                     /translation="MSVREFFKSRVFWKQLGLAMVIGVAIILILIIWLNIYTRHGQAR
                     PTPEVRGLSIREAQHVAGKNRMKLQIIDSVYTTMVPRGCVAEQTPLPGHRVKKGRTIK
                     ATINAFNPEMVAVPDLVGLPRRQALSIIETAGLQVGQLNYVADLTVDFVLKQTIHGRE
                     ASPGDSVQKGMVVDLVLGRGLSSQRTSLPRLTGLTLDEARNEILGASLNLGAFVFDGT
                     VITHDDSLGAFVFKQNPEYHSQASVQLGSAVYIWLTTDSLRMPSDTISADTLLPEGDF
                     GPPDAEIPR"
     gene            complement(4102..5211)
                     /locus_tag="PKK03_10795"
     CDS             complement(4102..5211)
                     /locus_tag="PKK03_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HNX84926.1"
                     /translation="MKNLLIKIPNPELRNLAAAVTDGKRLTPAEALTLYRNADLSLMA
                     LLATTVKRKKSGDAVYFNRNFHIEPTNICIYNCRFCSYHKAEGDPGSWEMTENEILDA
                     VRHFDGIPVTEVHITGGVHPSRDIYYYARILSSIRKLRPDVRIKAYSAVELDYMIREA
                     GMTYTDGIRLLHEHGLDSIPGGGAEIFDPELRRRICPEKADAETYLAIHEAAHRLGLS
                     SNATMLYGHAESPEQRIDHMLRLRDLQDRTGGFNAFIPLKFRRENNRMSELGEVPVTE
                     DMRNYALSRIFLDNFSHIKAYWPAIGRNNAMMSLAFGTDDLDGTIDDTTRIYSMAGSG
                     EDRPSLSTTELVAMIRSAGFRAVERDTNYNTVKEW"
     gene            complement(5314..5472)
                     /gene="rpmH"
                     /locus_tag="PKK03_10800"
     CDS             complement(5314..5472)
                     /gene="rpmH"
                     /locus_tag="PKK03_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNX84927.1"
                     /translation="MKRTFQPSRKKRTNTHGFRKRMSTPNGKRVLAARRAKGRKKLTV
                     SDEVSHKD"
     gene            complement(5600..6223)
                     /gene="rsmG"
                     /locus_tag="PKK03_10805"
     CDS             complement(5600..6223)
                     /gene="rsmG"
                     /locus_tag="PKK03_10805"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNX84928.1"
                     /translation="MTTSAEKYFPSLTPLQRHQFSLLPEIYREWNNRINVVSRKDIDN
                     FEVNHLLHSLAIARFIRFAPGTSVIDIGTGGGLPGIPLAVFFPDVRFTLADSIAKKIR
                     VASAVATEAGLNNVTAVTARAESLSGQYDFIIARAVTETAQFVRLTRHLASRKSFNEK
                     KNGYILLKGGNLEEELGPFLKSVEIADISNWFEEPWFETKKVVYLPF"
     gene            complement(6220..>6791)
                     /locus_tag="PKK03_10810"
     CDS             complement(6220..>6791)
                     /locus_tag="PKK03_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX84929.1"
                     /translation="AQYDLKVIDRALHGDSRAYTELLNRYRDSVYYVILRMVNNPSDA
                     EDLTIEAFGKAFHNLAKYVPSHAFSTWLFRIATNNCIDFMRRKSQSPRPFDQDEGEED
                     EVEATVASDMIAPDELMINRETAASLNRIVKTLKPRYRRLIELRYFEDYSYEEIASEL
                     SLPIGTVKARLFRAKVLILNMVQSKSSGI"
BASE COUNT         1485 a         1713 c         1851 g         1742 t
ORIGIN      
        1 ttgtcaccgg catttgtccg cacagcgata taactctcaa tgccagattc ctggtacgaa
       61 gctgcatttg ttccttcaga cccataatta ttccacgtaa ttatgactct gaaagtttat
      121 caaaaattac tgaaatcctg aatcctaaat cctaaatcac aaatcgctcc attaaaacgc
      181 aaatcgcaaa acgcaaatcg ctccattaaa tcgcaaatcg caaatcgcaa atccattact
      241 ctctgccagc aatataattc ctccacttct caataacaac aaccatatcc tgagggacgg
      301 gtgactcgaa ggagaggcgc ttgccggtga caggatggtc aaaggagagg gtgcgggcat
      361 gaagcgcctg gcgcggaagc agatcgaagc agttgcggac gaactgctgg tattttgtaa
      421 aggtggtgcc tttaagtatt cggtgaccgc cgtactcctc gtcgttgaag agcggatgcc
      481 ccttccaggc catatgcacc cttatctgat gggtccggcc ggtctcgaga cgacattcaa
      541 tcagtgacac ataccccagc ggttcgatga tcctgtaatg ggtcactgca tgcttcccct
      601 ggctgccgtc agggaatacc tgcatgatct ttcggtctcg aatattgcgg ccgacatggc
      661 cggtaatggt cccttccgaa ggatcaggtg taccccatac cagggctgtg taaagcctcc
      721 ctgtggtacg tctgaagaac tgcctggcca gcctgttgtg agcgtactcg ttcttggcca
      781 caaccagcag tcccgatgtg ttcttgtcaa tgcggtgcac cagccccgga cgaatctctc
      841 ccgtctggaa gagcggcagg tccctgaagt gatacatcag ggcgttcatg agtgtgcccg
      901 agtagttccc ataggccgga tgaaccacca ttcctgcggc cttgtcaaca actatcaggt
      961 catcatcttc ataaacaacc gtgacgggaa tatcctgcgg tataagttca atctcccggg
     1021 gcggattggg aaggaggacc tggattacat caagtggttt gacccggtag tttggcttga
     1081 cgggggaatc gtttaccagg atgtttccgg cctgggccgc agcctgcacc cttgtgcggg
     1141 atacgttctc tatccgtttg gagaggtatt tatcgatcct gagcatcgat tgcccggggt
     1201 caacaacgta ccgataatgc tcatatagtt cctgttcctg gccttcctgg tcaggttcgg
     1261 tgtatttttc ctgttccctg gtatcggaga attcctccac agtcatcccc ggatttactt
     1321 gactaataca actcgcagta gcgacagggg ccggccatcc ctcgtcctga tcaggagcat
     1381 atagttcccg gcggccagcc ggctcaggtc gggcctcttg ggatttccgg gcgacatcac
     1441 cacggctcct gaagatgaga taacgtcaat ggtgaagtca tcaggcgccc cttctgccgg
     1501 gaccagggtt accaggccgt cagtcggatt gggataaagg gagaagaggt tattgatcag
     1561 ggtgccgtta tctgatgaat ttggtatgcc gcttcccttc atcaccggcc gcatcattat
     1621 tatgccggga gcctggctct cctgccatat gccgctgaga tgaaagtatt gccttcccgc
     1681 cggggcggtg ttaaggtcca gtccggcatt gagaaatttc tcggaattct gtcgccagcc
     1741 aatccagaag ttgtcagtca ccctcaccgg agtgtcaaac atgtaggtca cgaaaccgtt
     1801 tatttctcct gccggagagg ccacgggacc gtcactgctg cccagcaatg cgccggggcg
     1861 accgttgacg tcagcccaga ccattatgtc gaatgccctc tggttggcgt tgtcatacgc
     1921 gtcattgaag cagaaactga tacctgtgac ggagtccggc aggaaagatc tgaaccggag
     1981 ggcagccatg gcgttgttcg agccctggcc attgatgccg taacctgctt ctgcggttcc
     2041 gtcgtcaaat gcgaaatagt cagaaaactt ctggatatat ctcagggtgt cattctgttt
     2101 gggatcgaag tcgtccgtta tcagtgatat tgtaaccatg aactgcgcgg tgtctgcaga
     2161 tgcggtcgta ttataggtat agagcagcgg agcgttatag ctgacatcgg aaagaggctg
     2221 ggcgttggct gctcctgcgt caaaatcgcg taccacggta ttgtctgaca tgtctgtgat
     2281 ggtgacatgc ctggtgacgt tgcggacgat ggtgtcattg ttccggtatt ttaccggagc
     2341 cacgggactc atggccgaga ggtatgcctg tctgaaatgg cgccatggca ttgcctcata
     2401 ttcctttaca agtgatcttg gtggaagggt gagggcaaca tcacgaagga cggtatcatg
     2461 aaccgaccgt cctttgtcaa gcagtacgtg gtcaatattc caatggtcag cgtttcctgc
     2521 ctgtgaaggt tcggagatca ctccggcaag agatgcgtgg ccggtgaaca tgaaccggaa
     2581 tcctttcatc aggtatctgg tgtcagtgac aggtatgatc accatgttga atccgttggt
     2641 tgcttctcct gtcgcctgcc agatgctgta ccacttctgc tcttccgggg cccagaagct
     2701 gagtgtgagg ctgtcgcctg cctcaggcag gtcagccacc cctccggctt caaaaaggaa
     2761 gctgaggaat atactgtccg aaggctggta ttcgaggtca atggtgcgcg atgtgagccg
     2821 gtcagcaggg aatactgcct gtgaagcccc ctcgtgcagc aatcctcttt catcaaggca
     2881 gtcaagggtt gcaatgccgg cagaaggctg tctgacagag taggtgttat ttataaatgc
     2941 ctgcaggtca gcccacctgg tgactgaagg aaaaatgctg tcacctgtaa agtcatcgct
     3001 gaagggcaga ggcacgggtt cttcggccgt tgcaccctta aggacagagg caggtctgaa
     3061 agcatcagaa acctgcctgt tgcggataag gccggtaatg acctcctgtc ctgacacatc
     3121 aagggtgagt acagcagcaa gcagaaggaa tatggcccgg ctgtttttca tctttagcgt
     3181 ggtatttctg catccggtgg tccgaagtca ccttcaggga gcagggtatc agcactgatt
     3241 gtgtcagacg gcattctgag actgtcggtg gtgagccaga tataaacggc agatcccagc
     3301 tgtactgatg cctggctgtg gtactcgggg ttctgcttga agacgaaagc cccgagggag
     3361 tcatcgtgtg taatgactgt gccatcgaag acgaaggcac caaggttgag ggaagctccc
     3421 agtatctcat tcctggcttc gtcgagtgtc aggccggtga gcctgggaag ggatgtgcgc
     3481 tgactgctca gaccgcggcc cagcaccagg tcaaccacca tccctttctg gacggagtcg
     3541 ccgggtgatg cttcgcgacc gtgaatcgtt tgtttaagaa cgaaatcgac cgtcaggtca
     3601 gcgacgtagt taagttgtcc aacctgcaga ccggctgtct caatgattga gagcgcctgg
     3661 cggcggggca gtcctaccag gtcaggaact gcaaccatct cggggttaaa tgcgttaatg
     3721 gtggccttga tcgtacgtcc tttcttgacc ctgtgaccgg ggagaggagt ctgttcggcc
     3781 acacatccgc ggggaaccat tgtggtataa accgagtcta ttatctgcag cttcatcctg
     3841 ttcttgccgg caacatgctg ggcctccctg atggacagtc ccctgacctc gggagtgggc
     3901 cttgcctggc cgtggcgggt atatatatta agccagatta tgagaatgag aatgatggca
     3961 acgcctatga ccattgccag gccaagctgt ttccagaaga cccggctttt gaagaattcc
     4021 ctgacgctca tcgtgtgatg aagtattagt ttaataacgg tgcctcaaag ataaaaatta
     4081 tatcgcggga acgagggatt tctaccactc cttcactgta ttgtaattgg tatcacgttc
     4141 cactgccctg aagccggctg accgtatcat ggccaccagc tctgtggttg agagtgatgg
     4201 tctgtcctca ccggaacctg ccatcgaata tattcttgtg gtgtcatcta tggtgccgtc
     4261 gaggtcatcg gttccgaatg caaggctcat cattgcgttg ttccggccta ttgccggcca
     4321 gtatgccttg atatggctga agttatcgag gaatatgcgt gacagggcgt agttgcgcat
     4381 gtcttccgta acaggcacct caccgagctc tgacatgcgg ttgttctcgc gcctgaactt
     4441 gagtgggatg aaggcattga agcctccggt gcggtcctgc aggtccctca ggcgaagcat
     4501 gtggtcaatg cgttgttcgg ggctctcggc atgaccgtag agcatggtgg cgttggagct
     4561 cagtccgagc ctgtgtgcgg cctcatggat ggcaaggtag gtttcggcgt cagccttttc
     4621 agggcatatc cggcgtcgca gctcaggatc gaatatctcc gcccctccgc cgggtatgga
     4681 gtcgaggcca tgttcgtgaa gcaaccgtat cccgtcggta taagtcatgc ctgcttctct
     4741 tatcatataa tcaagctcca cggcggaata ggctttaatt ctgacatcag ggcggagctt
     4801 ccggattgaa gagagaatac gggcgtaata atatatgtca cgcgagggat gcacccctcc
     4861 ggttatatgg acctcggtga caggtatgcc atcaaagtgg cggactgcat caagtatctc
     4921 attttcagtc atctcccagc tgccgggatc gccctcggcc ttatggtatg aacagaaccg
     4981 gcagttatag atacagatat ttgtaggctc aatgtggaaa ttcctgttga agtagacggc
     5041 gtcaccgctt ttttttctct tgacggtggt agccagcagc gccatcagtg aaaggtcggc
     5101 attccggtat agagtgagcg cctcagcggg tgtgagccgt ttcccgtctg ttactgctgc
     5161 tgccagattc cttaactcag ggtttggaat tttgattaac aggtttttca ttcttcagat
     5221 tccctgcaaa aatagctcag ggatgcataa cagaaataaa aaaccgcctt aattgttcat
     5281 caagacggcg attatttatt gtctgtcagc ctgtcagtcc ttgtggctca cctcgtctga
     5341 aaccgtgagt ttcttccttc cctttgccct tcgtgctgca agaacgcgct tgccattggg
     5401 agttgacatt ctcttccgga agccgtgagt gtttgtcctt ttctttctcg atggctgaaa
     5461 tgtccttttc atctgatgct atatttcgtt gttaactatc cggtttttga gactgcaaag
     5521 gtagtcattt tttcaaataa agaagtccgg aggctacttt ttttaatatt aagcatttcc
     5581 ccggtgccgg gggaataact cagaagggca ggtacacaac cttttttgtt tcgaaccatg
     5641 gttcttcgaa ccagttgctg atgtccgcaa tttcgacaga cttcaggaag ggaccgagtt
     5701 cctcctcaag gtttcctccc ttgagaagga tatatccgtt tttcttctca ttgaatgatt
     5761 tccgcgaagc cagatgccgt gtcagcctca cgaactgcgc tgtttcggtc accgccctgg
     5821 cgataatgaa atcatactgc cccgaaagac tttccgccct cgctgtcacg gctgtgacat
     5881 tgttcaggcc ggcttccgtg gcgacagctg aggcaaccct gatcttcttg gcaatggaat
     5941 ctgccagggt gaacctgacg tcaggaaaga acacggccag aggtattccc ggaagtcctc
     6001 cgccggtgcc gatgtcaatg acagatgttc ccggcgcgaa cctgatgaaa cgggcaatgg
     6061 caagggaatg aagcaggtgg ttaacctcaa agttgtcgat atccttccgg gaaacgacgt
     6121 tgatcctgtt gttccactcc ctgtatattt caggcaggag tgagaactga tggcgctgca
     6181 aaggggtgag tgacgggaag tatttttcgg ctgatgtggt caaatgccgc tcgatttgct
     6241 ttggaccatg ttcagtatca ggacttttgc cctgaagagc ctggctttga cagtgccaat
     6301 gggaaggctc agttcactgg ctatctcctc gtaggaataa tcctcgaagt agcggagttc
     6361 aatcagcctg cggtagcgcg gcttgagggt tttcactatc ctgttgagtg aagctgccgt
     6421 ttccctgttg atcatcagtt catcgggagc aatcatatca gaagccactg ttgcctcgac
     6481 ctcgtcctct tccccttcat cctgatcgaa tggcctggga ctctggcttt tacgcctcat
     6541 gaaatcgata cagttattgg tggctatcct gaacagccat gtgctgaagg catgtgacgg
     6601 cacgtatttg gcgaggttat ggaacgcctt gccgaaggct tctatggtca gatcctctgc
     6661 atcggaggga ttgtttacca ttctcaggat gacgtaatag acggagtcac ggtaacggtt
     6721 cagcagctcg gtgtatgccc tgctgtcgcc gtgcagtgca cggtcgatga ccttgaggtc
     6781 atactgcgct t
//
LOCUS       DAOPXU010000131         6749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_13797_length_6749_cov_11.964745, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000131 DAOPXU010000000
VERSION     DAOPXU010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13797_length_6749_cov_11.964745"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..1352
                     /locus_tag="PKK03_10815"
     CDS             42..1352
                     /locus_tag="PKK03_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84930.1"
                     /translation="MEIIGKLTYRRILLFWGPLALTWLMMAFEQPFLIAFIARLGDAK
                     VNLAAFGIAGSFAMIFEAPVIMLMSTSTALVTGHHSYRKLKHFTDLLNSGVTLVQLIV
                     LIPPVFRFIIINLMGVPEEVAGVAHTALLIFLPWAASIGYRRFYQGILIRNNLTRRVT
                     YGTMVRMTVIVITGVLLYSAGVRGAYVGAGAMSLAVFSEAIATRLMAASTVRALVIKD
                     DTENGHLNLRNILKFYYPLALTSLLSLGVHPFVTFFLGRSTMAVESLAVLPVVNSLVF
                     IFRSLGLSYQEVVIALIGKEKQNYSMLRNYAVRLGIIVTVLISVIAFTPLADLWFTDI
                     SGLDGELAGLSYLPLQIMILLPALTVLLNFQRSLFVVNGTTGPISAATAVELVGIIAV
                     LLVCVIFLGMVGVFAASIAFVAGKGMSTLYLLPGQSALLKRWRG"
     gene            1407..1823
                     /locus_tag="PKK03_10820"
     CDS             1407..1823
                     /locus_tag="PKK03_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNX84931.1"
                     /translation="MKSYRKEFWIETLHRRDYVNITRQVEKCLDESGIREGMVLVNAM
                     HITASVFINDDDNGLHNDFEEWLEKLAPEKPHSQYRHNSFEDNADAHMKRQLMGREVV
                     IAVTNGKLDFGPWEQIFYGEYDGMRRKRVLVKIIGE"
     gene            complement(2014..3747)
                     /locus_tag="PKK03_10825"
     CDS             complement(2014..3747)
                     /locus_tag="PKK03_10825"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNX84932.1"
                     /translation="MINIEEIRKKASAWLGEEFDEPTRNEVKHMMDHDEKGLIDAFYQ
                     DLEFGTGGLRGIMGAGTNRMNRYTLGMATQGLSNYLKKHAANPSSIKVAIAYDCRNNS
                     SYFAKVAADIFTANGFTAYLFESLRPTPELSYAIRLYGCDAGVIITASHNPKEYNGYK
                     VSWNDGGQVVSPHDRGIINEVRSIRSVTEIAGGGDPSKIVMLGEETDKKYLDEVLKLS
                     LNPEIIRRNADMGIVYTPLHGTGYKMVPQALRMYGFKNVYSVEAQSITDGNFPTLRSP
                     NPEEPDALAMALSLAREKNADLVMATDPDADRLGIAVRKPSGEFVLLNGNQTGALLTW
                     YIISQKKQNGDLKGNEYIINTIVTSDLLDRIAEKNGVKSYNVLTGFKYFAALMRDLEG
                     KEKYIGGGEESFGYLPGDYVRDKDGVASCALVAEAAAWAADHGKSIWELLLDIYAGYG
                     LFREKLVNVVRKGAEGAAEIKSMMEGYRTSPPVSIAGSRVARINDYQLQVSTDPATGI
                     KTPLDFEKSNVLQFFLADGTKISVRPSGTEPKIKFYFSTSTVMNETSQFEELWQQLDE
                     QIDAVIKDMKL"
     gene            complement(3744..4616)
                     /gene="rfbD"
                     /locus_tag="PKK03_10830"
     CDS             complement(3744..4616)
                     /gene="rfbD"
                     /locus_tag="PKK03_10830"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNX84933.1"
                     /translation="MMTILVTGASGQLGSEIRELSARFSGYEFIFTDTGELDITDAGA
                     TAEFIAANAPSWIINCAAYTAVDRAEDEEKLATIINAGGVGNLVSALRGTDCRLIHIS
                     TDYVFDGNIPVPYTEYDTPSPASAYGRSKLAGEKAALTWPGTMIIRTSWLYSSYGNNF
                     VKTILRKAGSSHDISVVFDQVGSPTYAADLANAIMEIISGVIRNKHNFVPGIFNYSNE
                     GVCSWYDLAIEIIREAGSQCSVIPVRSSAWPSKVKRPAYSVLDKTRIKESFNLTIPHW
                     RTSLNNCISKINRI"
     gene            complement(4613..5173)
                     /gene="rfbC"
                     /locus_tag="PKK03_10835"
     CDS             complement(4613..5173)
                     /gene="rfbC"
                     /locus_tag="PKK03_10835"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNX84934.1"
                     /translation="MQVHKTEFPGLLIIEPDVFYDARGYFFESYNRLKYSNNEVVYEP
                     VQDNESSSTRGVIRGLHYQEEPFAQAKLIRVIEGRIYDVALDIRRGSPTFGKWFGLII
                     DSEKKLQFLIPKGFAHGFSVLSERAIVLYKCDNTYSVEHERGINALDPALGIDWMLRD
                     ETPVISPKDLKQPLFKDISTNFIFRA"
     gene            complement(5176..6048)
                     /gene="rfbA"
                     /locus_tag="PKK03_10840"
     CDS             complement(5176..6048)
                     /gene="rfbA"
                     /locus_tag="PKK03_10840"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNX84935.1"
                     /translation="MKGIILAGGSGTRLHPITEAISKQLLPVYDKPMIYYPLSALMLA
                     GIREVLIISTPRDLPSFRALLGDGSRLGMNFTYREQPSPDGLAQAFIIGEDFIGDDHV
                     CMILGDNIFYGQGFGSILKQAAMLKEGAVVFGYAVNDPERYGVVEFDADLKAVSIEEK
                     PLKPKSNYAVTGLYFYDNTVAGRARNLKPSPRGELEITDLNRLYLQEGKLEVKLLGRG
                     MAWLDTGTFDSLLQASNFIATLEQRQGLSASCIEEIAYKNGFITREKLVDLAKPLAKS
                     QYGQYLLKVANEQN"
     gene            6253..>6749
                     /locus_tag="PKK03_10845"
     CDS             6253..>6749
                     /locus_tag="PKK03_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNX84936.1"
                     /translation="MSENNENAGTVSNEEVKVNETGAEEAKSKEVVVESAGEMVAEEQ
                     PILKEKKEKKVKAEAPSTAAAAAALTPEEFDWDAYEQDGFSSKRSRADQEKIYEETLS
                     TVAVDEVVDGTVISMNNREVVINIGQKSEGVVSLNEFRYNPELKVGDKVEVYVESQED
                     KKGQLV"
BASE COUNT         1551 a         1679 c         1776 g         1743 t
ORIGIN      
        1 ggattattcg acgtttatta tctttgcgct ttcgcttacc ggtggaaata atcgggaaac
       61 tcacataccg caggatactc cttttctggg gaccgctggc actgacctgg ctgatgatgg
      121 ccttcgagca gccattcctg atcgccttca tcgcccgcct gggtgacgca aaggtcaacc
      181 tggcagcctt cggcattgcc gggtctttcg caatgatctt tgaggctccg gtaataatgc
      241 tgatgagcac ctccacggcc ctggtcaccg gacatcattc atacaggaaa ctgaaacact
      301 tcactgatct gcttaactcc ggtgtcacgc ttgttcagct gatcgtgctg atccctccgg
      361 tcttcaggtt cattatcatc aacctgatgg gggtgccgga agaggtcgcc ggggttgcac
      421 ataccgctct tctcatcttc ctgccctggg cagcatcgat agggtacagg aggttctacc
      481 aggggatcct gatcaggaat aatcttacca ggcgggtcac atacggcacc atggtgagga
      541 tgacggtcat agtgattacc ggagtgcttc tctattccgc aggcgtcagg ggcgcttatg
      601 tcggcgcagg ggcaatgtca ctagcggttt tctcagaggc aatagcaacc cggttgatgg
      661 cggcatcaac ggtcagggct ctggtcataa aagatgacac cgagaacggg catctcaacc
      721 tgagaaatat cttgaagttc tattaccccc tggcacttac ctcacttctc tctctggggg
      781 tgcacccgtt tgtgacgttc ttcctgggaa ggagcacaat ggcagttgaa tctcttgccg
      841 tgctgccggt ggtgaactca ctggtgttta ttttcaggag ccttggcctg tcataccagg
      901 aagttgtcat tgcccttatc gggaaagaaa aacagaacta cagcatgctc cgtaattatg
      961 cagtccggct cggaatcatt gtgacggttc tcatctccgt cattgccttt accccgcttg
     1021 ccgatctgtg gttcactgac atctccgggc ttgacggcga gctagcaggg ttgtcatacc
     1081 ttccgctgca gataatgata ctccttccgg cgctgacggt gctgctaaac ttccagcgct
     1141 cgctgtttgt ggtcaacggt acgacaggac ccatatctgc agcaacagcc gttgagctcg
     1201 tcggaattat tgcagtattg ctggtttgcg tgatattcct cggcatggtg ggtgtgtttg
     1261 ccgcatccat agcgtttgtg gcaggcaagg ggatgtcaac cctctatctt ctgcccgggc
     1321 agtctgcact gctaaagagg tggaggggat agattatttg cagaattctc tgagtatatt
     1381 tgtataagag aatcaatttc accgtcatga aatcataccg caaggaattc tggatcgaga
     1441 cactgcatcg cagggattat gtcaatatta cccgccaggt tgaaaagtgc ctggatgaga
     1501 gcggaatccg ggaaggcatg gtactcgtca atgccatgca tataacggca agtgtattca
     1561 tcaatgacga cgacaacggg ttgcataacg acttcgagga gtggctggag aagctggcgc
     1621 cggagaagcc tcactcacaa taccggcaca acagctttga ggacaacgct gatgcccaca
     1681 tgaagcgcca acttatgggg cgtgaggtgg tgattgccgt aaccaatggc aaactcgact
     1741 tcggtccgtg ggagcagatc ttctacggtg agtatgacgg catgcggagg aaacgggtgc
     1801 tggtgaagat aatcggggaa taggccggag gcaacaggca gtcggcagta ccagtcggca
     1861 gtcggcagta ccagccggca gtcggcagtg ccagtaggta gtcggaagta gtagtgcctt
     1921 ctgtaataca gatagttagg ggctgaatac tgcatgaagc cacctgcagt ctgcaaaatt
     1981 cactattccc tgttgcctgc tgcctattgc ctgctagagt ttcatgtcct taatgacggc
     2041 atcaatctgc tcatctagtt gctgccacag ttcctcaaac tgagaggtct cattcatgac
     2101 agtgctggta ctgaagtaga actttatctt cggttcggtt ccggaaggac gtacggaaat
     2161 cttcgttccg tcggcaagga agaactggag gacgtttgac ttttcaaagt caaggggtgt
     2221 cttgatcccg gtggcaggat cggttgagac ctgcaactgg taatcattta tcctggccac
     2281 cctgcttcct gcaatagaca ccggcggact ggtgcggtat ccttccatca tgctctttat
     2341 ctcggctgct ccctcggctc cctttctgac cacattgacc agtttttccc tgaagagacc
     2401 gtacccggca taaatgtcga gcagcagttc ccagatcgat ttgccatgat cggcagccca
     2461 tgcggctgcc tcagcgacga gggcgcatga tgcaacgccg tccttgtccc tgacataatc
     2521 gcccggcaga taaccgaagc tctcttcgcc tccgccgatg tatttttcct tcccttcgag
     2581 gtcacgcatg agagctgcga aatatttgaa tcccgtgaga acattatagc ttttcacacc
     2641 gttcttttcg gctatcctgt caagcaggtc ggacgttacg atagtattaa taatgtattc
     2701 attgccttta agatcaccat tctgtttctt ctgggagatg atgtaccagg tcagcaatgc
     2761 acctgtctgg ttcccgttca gcagcacgaa ctcgccggaa ggttttctca ctgctattcc
     2821 gaggcggtca gcatcagggt ccgtagccat caccaggtcg gcatttttct cgcgtgccag
     2881 gctgagggcc attgccagag catcaggctc ctcgggattg ggtgaacgca gggtagggaa
     2941 gttgccgtca gtaatacttt gggcttcaac tgaataaaca tttttaaagc cgtacattct
     3001 cagcgcctgc ggaaccattt tataccctgt gccgtgaagg ggagtataga cgattcccat
     3061 gtcagcgttt ctccggatta tttccggatt gaggctcagc ttcagcactt catccaggta
     3121 ctttttgtct gtctcttcgc caagcatcac tattttggaa ggatcaccgc caccggctat
     3181 ctcagttact gacctgatgc tccggacctc attgatgatt cccctgtcat ggggagagac
     3241 cacctggccg ccgtcgttcc atgaaacctt ataaccgttg tactctttag ggttgtggga
     3301 ggcggtgata atgacaccgg cgtcacaacc gtacagtctg atggcatatg acagctccgg
     3361 tgtcggcctc agagactcga acaggtatgc ggtaaagccg ttcgcggtga agatatctgc
     3421 ggcgactttg gcgaaatagc tgctgttgtt gcggcagtca tacgctattg ccaccttgat
     3481 ggatgacgga ttggcagcat gttttttcag gtagtttgac agtccctgtg ttgccatccc
     3541 cagggtgtaa cggttcatcc tgttggtgcc ggcgcccata ataccgcgca gaccgccggt
     3601 gccgaactca aggtcctggt agaaagcgtc gatgaggcct ttctcgtcgt gatccatcat
     3661 atgtttcacc tcgttgcggg ttggttcgtc gaactcttct ccgagccatg ccgaggcttt
     3721 cttcctgatt tcttcgatgt tgatcatatc ctgtttattt ttgagataca gttgttaagg
     3781 cttgtgcgcc aatgcggtat tgtgaggttg aaagattcct ttatccttgt cttgtcaagt
     3841 acgctgtatg ccggcctttt tacctttgag ggccatgctg agctcctgac ggggataacg
     3901 gagcattgtg accctgcctc cctgattatc tcgatggcaa ggtcgtacca gctgcagacg
     3961 ccctcattcg aatagttgaa gattcccgga acgaagttgt gcttattgcg gatcacgccg
     4021 ctgataattt ccataatggc atttgcaagg tcggcagcat aggtcggaga acccacctgg
     4081 tcaaagacca cactgatgtc atgcgacgat ccggctttgc ggagaatggt ctttacgaag
     4141 ttgttgccgt aggaggagta gagccatgat gtcctgatga tcattgtgcc gggccaggtt
     4201 aaagcggcct tttctccggc cagtttgcta cgcccgtaag ctgatgccgg cgacggggtg
     4261 tcatactcgg tatatggcac cggaatgtta ccgtcgaaga cataatcggt cgagatgtga
     4321 atcagcctgc aatcggttcc cctgagggcg ctgaccagat tccccacccc tccggcgttg
     4381 atgatggttg ccagcttttc ttcgtcctca gccctgtcaa cagcagtgta tgcggcgcag
     4441 ttgatgatcc atgaaggggc atttgcagca atgaattcag ctgtcgcccc ggcgtcagtt
     4501 atgtcaagct caccggtatc ggtgaagatg aattcatagc cgctgaaacg ggctgacagt
     4561 tccctgatct cacttcccag ttgtccggat gccccggtca ccagtatggt catcatgctc
     4621 tgaagatgaa gttcgttgaa atatctttga acagaggctg tttgaggtcc ttgggactta
     4681 tcacaggtgt ttcgtccctg agcatccagt cgatccctag agcagggtcc agggcattga
     4741 ttcccctttc atgttcaacc gagtaggtgt tgtcacattt gtacagcact atggcccttt
     4801 cactgagcac ggagaagccg tgggcaaatc ctttgggaat gaggaactgc agtttcttct
     4861 cagagtcaat tatcagtccg aaccacttcc cgaatgtggg ggagcccctg cggatatcaa
     4921 gggcaacgtc atagatccgc ccctcaatca cccttatcag ctttgcctgg gcaaaaggct
     4981 cctcctggta gtggagtccg cggatgacac ccctggtgga acttgattcg ttgtcctgga
     5041 caggttcgta aactacttcg ttattggagt atttcagcct gttatagctt tcaaagaaat
     5101 aaccgcgggc gtcataaaag acatccggtt cgatgattag aagcccgggg aattcagttt
     5161 tgtgtacctg cattttcagt tctgttcgtt tgccactttc agaagatact gaccgtactg
     5221 gcttttggcc agtggtttgg ccagatcgac cagtttctcc ctggttataa atccgttctt
     5281 gtaggcgatc tcctcgatgc aggatgcgct gagcccctgc ctctgctcca gcgttgcaat
     5341 gaagttggac gcctggagga ggctgtcaaa tgttcctgta tcaagccagg ccatcccgcg
     5401 accgagaagt ttcacttcaa gcttgccttc ctgcaggtag agcctgttga ggtcagttat
     5461 ttcaagctct ccgcggggtg agggtttaag attgcgtgcc cttccggcaa cggtattatc
     5521 atagaaatac agccctgtca ccgcatagtt tgatttgggc tttaaaggtt tttcctcgat
     5581 gcttacagcc ttcaggtcag catcaaattc aaccacaccg taacgctccg ggtcattgac
     5641 agcatacccg aagactacgg caccctcctt cagcattgcg gcctgtttga ggatgcttcc
     5701 gaaaccctgg ccgtaaaaga tgttgtcacc cagaatcatg cagacatgat catcgccgat
     5761 gaagtcctct ccgatgatga atgcctgtgc cagcccgtca ggagagggct gctcgcggta
     5821 agtaaagttc atccccagcc ttgatccgtc acccagcaat gccctgaaac tcggcaggtc
     5881 acgcggagtg gagatgatca gcacctcgcg gatgcctgca agcataagag ctgaaagcgg
     5941 gtagtagatc atgggtttgt catagacggg cagcagctgt ttggaaatgg cttcggtaat
     6001 agggtgaagc ctggtgccgg acccgccggc aaggataatg cctttcatca gcaaatattt
     6061 agttatacag atgacaaaaa tagaaaaaag agccaatcta aacagtgacc ccagcgcttc
     6121 acctgttttt aattcttata tcctgaaaat ctgttctttg ttcaacgccg gatcaaaaaa
     6181 ataattatct ttgcaacctg tttttaatca acataatgtc taacttatta attttcaatt
     6241 aatttattaa agatgagtga aaataacgaa aatgcaggca ccgtttcaaa cgaagaggtt
     6301 aaagtgaatg agaccggtgc ggaagaagca aagtcaaaag aggttgtggt tgagtcagca
     6361 ggcgaaatgg ttgctgaaga acagccaatc ctgaaggaaa agaaagaaaa aaaggtgaag
     6421 gcggaagcac cttcaacagc agcggcagca gcagccttaa cacctgaaga gtttgactgg
     6481 gatgcctatg agcaggacgg attctcgtcg aagaggagcc gcgctgacca ggagaagatt
     6541 tatgaagaaa ccctttcaac agtagcagtt gacgaggttg ttgatggcac agtcatttca
     6601 atgaataacc gtgaagtggt tatcaacatc gggcagaagt ccgagggcgt ggttagcctt
     6661 aacgaattcc gctacaatcc cgagctcaag gtaggagaca aggtagaggt ctatgttgag
     6721 agccaggaag acaagaaggg ccagctggt
//
LOCUS       DAOPXU010000132         6688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_13971_length_6688_cov_10.845620, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000132 DAOPXU010000000
VERSION     DAOPXU010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6688
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13971_length_6688_cov_10.845620"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="PKK03_10850"
     CDS             <1..1068
                     /locus_tag="PKK03_10850"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HNX84937.1"
                     /translation="FTSFFTFARVKNDHLSVELGIDAFAELGRAMREGATGSGTGSGK
                     RFHDLSATLYLSNPWFTPDNVRRALFATGAVLTKEKLTRWLSAYPELSEERNPVTVGV
                     VMAGNIPMVGFHDLLCVLISGNRLLARLSTRDELMMRAVAATLTEIEPAFRHVTELTT
                     DKLKGFGKVIATGSNNTSRYFEYYFRNVPSIIRRNRNSIAILDGTESDGELELLGDDV
                     FSYFGLGCRSVSKLYLPRGYDPASLLPRWERHESLCSHYRYMVNYDHHKAVMIVNRES
                     FTDGRFILLKESSSFTPPMAVLHYEFYDSGTGPAITPEMSDGMLQCIAGHGHLPFGKT
                     QKPELWNYADNVDTISFLLKK"
     gene            1127..1702
                     /locus_tag="PKK03_10855"
     CDS             1127..1702
                     /locus_tag="PKK03_10855"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84938.1"
                     /translation="MVYRFVVLSDEDEAFAREFEFLDSQSLLDFHNALQEELEFDRSQ
                     IASFFMATESWEKEEEFTLFDMGAGSSTMENAVLEEIIFRKNQKLLYVFDFFNDRALY
                     VEYVGEFKEEDDKEYPVCINSKGLPPKQVTFGGVSRKKYNNLVVTDDDDDDDVVDEEV
                     VDDEIFFEGEDEDDLSEFDNIENSDEEEEEE"
     gene            1724..2638
                     /gene="miaA"
                     /locus_tag="PKK03_10860"
     CDS             1724..2638
                     /gene="miaA"
                     /locus_tag="PKK03_10860"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNX84939.1"
                     /translation="MKNTLIVLPGPTGVGKTAFATGLAERLGCEIISCDSRQFYREMK
                     IGTAAPGKEELARVRHHFVGFLSVTDYYSISLFERDVLALLPSLFSSNPVAIMTGGSM
                     LYMDAVCRGMDDIPDTDPAVRQKYLGMYHDEGIEGLRIALKMLDPASYSRIDLHNPRR
                     ILRALEITESSGRPYSSFLTAPVRQRDFRIIKAGLTRERGELYRRIDMRVDRMMEEGL
                     EQEAVSLKQYRELNALNTVGYREMFSWFDGEVSRDQAVMLIKRNTRRYARKQLTWWNR
                     DNDINWFDVSEREMIVSWIESRLEENCS"
     gene            complement(2682..3335)
                     /gene="rpe"
                     /locus_tag="PKK03_10865"
     CDS             complement(2682..3335)
                     /gene="rpe"
                     /locus_tag="PKK03_10865"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNX84940.1"
                     /translation="MSHLVAPSLLAADFANLASEVEMINDSEADWLHLDIMDGLFVPN
                     ISFGFPVMEAVRDLSSKPLDVHLMIVDPDRYLERFREAGASNLTVHFEACRNLHRTVT
                     EIRRLGMKAGVTLNPHSPVILLKDILPYIDMVLLMTVNPGYGGQTFIPGSFNKIGELR
                     SMIDEGGFDVMIEVDGGVDLHNAPMLVHAGVDVLVAGNTVFSSSDPAEVIRKLKQPV"
     gene            complement(3361..4224)
                     /locus_tag="PKK03_10870"
     CDS             complement(3361..4224)
                     /locus_tag="PKK03_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNX84941.1"
                     /translation="MRQLKITKQVTNRDTPSLDKYLQEIGKVELISPDEEVSLARRIR
                     TGDHDALARLVKANLRFVVSVAKQYQNQGMTLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQAILQSLAEQSRIVRLPVNKIGSINRINKAYSKLEQEYEREPSAQEIAD
                     LLEIAPEDVKEAIRTNGRTLSMDAPISSEEDNNMYDVMQNEETPSPDRNLINESLSYE
                     IEKALSTLSPREARVLKLYFGIGMKHPFTLEEIGEELKLTRERVRQIKEKAIKRIQFT
                     TRSRILKSYLG"
     gene            4561..>6688
                     /gene="rho"
                     /locus_tag="PKK03_10875"
     CDS             4561..>6688
                     /gene="rho"
                     /locus_tag="PKK03_10875"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNX84942.1"
                     /translation="MHDILELSEMLVPELREIAKQLKIKRVELLKKQDLIYKILDQQA
                     IDAAETRKTGRAENQPSDRKQEQSGQPNRQGKPDRPAKNPDQRRQQGQQNQGQQQQQP
                     RQQNLGRPPKPQHDQQQRKPQGQGQQQGQTQSQPQGQGQKQGQQQQQQGQQHGQPQPQ
                     SQGQGQSQGQKQGQQQQQGQQHPQGQQQNRQQQWQQNRDKRPRTFRNDQQGPYRHDQN
                     RQQQQQQARQDEMAQTPSQQPQQQSQPQPQQQSQQQSQQQPREQAYQNEQGLPPADKL
                     LFTPETVTDSDQDLLRFPEPEGESRIIEEAAAPEGAVVIPPVPAEASAPQPEVHEPVR
                     EEKKERAYDFEGIVANTGVLEIMPDGYGFLRSPDYNYLSSPDDIYVSQSQIKLFGLKT
                     GDTIRGTIRPPREGEKYFPLIKVEEINGRTPDFIRDRVPFDYLTPLFPNEKFTLCRPG
                     EGNLSVRIVDMFCPIGKGQRGLIVAQPKTGKTILLQDIANAIAKFHPEVYLMILLIDE
                     RPEEVTEMARNVNAEVIASTFDEPAERHVRIATIVQEKAKRLVECGHDVVILLDSITR
                     LARAFNTIAPASGKVLSGGVDSNALHKPKRFFGAARNIENGGSLTIIATALTDTGSKM
                     DDVIFEEFKGTGNMELQLDRKLSNRRIFPSIDIPASSTRREDLLLDKNMLNKIWILRN
                     YLTDMNSVEAMEFLRDRITQTRSNEEF"
BASE COUNT         1761 a         1689 c         1750 g         1488 t
ORIGIN      
        1 ttcacttcat tttttacctt tgcccgggtg aagaacgacc atctgtcagt tgaacttggc
       61 attgatgcct ttgcagagct gggaagagcg atgcgcgaag gtgcaaccgg ctccggaaca
      121 ggatccggaa aacgctttca tgacctttcc gcgacgctgt atctctccaa tccatggttc
      181 acacctgata atgtcaggcg tgctctcttc gcaaccgggg ctgttctcac caaagaaaaa
      241 ctgacccggt ggttgtcggc gtacccggaa ctcagtgagg aacgtaatcc ggtaacggtg
      301 ggggtggtta tggccggtaa cattccgatg gtaggattcc atgacctgct ctgcgttctt
      361 atatcaggaa accgcctgct ggccaggctg agcaccaggg atgagctgat gatgagagcc
      421 gtggccgcaa cactgactga aattgaaccg gcgttcagac atgttactga gctcacaacc
      481 gacaaactga aaggattcgg gaaagtgatt gcaacgggaa gcaacaatac ctcaaggtac
      541 tttgagtact acttcagaaa tgttccctcc atcatcagaa ggaacaggaa cagcatagcc
      601 attcttgacg ggacagagag tgacggtgaa ctggagctgc tgggtgacga tgtcttttca
      661 tacttcggac tgggttgcag gagtgtttca aaactgtacc tcccccgcgg atatgacccg
      721 gcatccctcc tgccccgctg ggagcgccat gaatcactct gctcccatta caggtatatg
      781 gtaaactatg accatcacaa ggcagtgatg atagtaaaca gggaaagttt cactgacggc
      841 cggttcattc tgcttaagga gtcgtcttca ttcactcccc cgatggctgt gcttcattat
      901 gagttctacg acagcggaac aggccctgcc ataaccccgg aaatgagcga tgggatgctg
      961 caatgcatag ccggacacgg acatctgcct ttcgggaaaa cccagaaacc ggaactgtgg
     1021 aattatgccg acaatgttga tacaatcagt ttcctgctaa aaaaataagt cggagagaat
     1081 ctattataaa aaatattaaa ttgcacaagt tgaaaaagtg agtgttatgg tatacagatt
     1141 cgtggtgtta tctgatgagg atgaggcgtt tgctagggag tttgagttcc ttgacagtca
     1201 atctctgctt gattttcaca atgccctgca ggaggagctg gagtttgacc gttcccagat
     1261 tgcttctttc tttatggcaa cagagagctg ggaaaaggag gaggagttca ccctgtttga
     1321 tatgggcgca ggctcatcaa caatggagaa cgctgtactt gaagagatca tcttccgcaa
     1381 aaaccagaaa ctgctctatg tttttgactt tttcaatgac agggcactct atgtagaata
     1441 tgtaggtgag tttaaggaag aagatgacaa ggagtatccg gtatgcatca actccaaggg
     1501 tctgcctccc aagcaggtga ccttcggtgg ggtttcccgc aagaaatata acaacctcgt
     1561 tgtcaccgat gatgacgatg atgacgatgt ggttgatgag gaggttgtgg atgacgagat
     1621 cttcttcgag ggtgaggatg aggatgatct gtctgaattc gacaacatag aaaacagtga
     1681 cgaggaggag gaagaggaat agtcgctcct tcacccggac cccatgaaaa atacactgat
     1741 tgtccttccc ggtcccaccg gagtgggtaa aacggcattt gccaccgggc tggccgaaag
     1801 gctgggctgt gaaataatat catgtgactc aaggcagttc taccgcgaga tgaagatcgg
     1861 aacggctgcc cctggcaaag aggaacttgc acgggtgaga caccacttcg tgggatttct
     1921 gtctgtcacc gattactaca gcatcagcct tttcgagagg gatgtgctgg cccttctgcc
     1981 atcattgttt tcgtcgaacc cggtggcaat catgacggga gggtcaatgc tatatatgga
     2041 tgcagtatgc aggggtatgg atgacatccc tgacactgat cctgccgtca ggcagaagta
     2101 tcttggaatg tatcatgatg aaggcattga aggtctgcgc attgcactga aaatgcttga
     2161 tcctgcgtcc tactcaagga tcgaccttca caatccccgt cgcatactcc gggccctgga
     2221 gataaccgaa agctccggca gaccctactc ttcgtttctt acggcaccgg tgagacagcg
     2281 cgatttcagg atcatcaaag ccggactgac ccgggagaga ggcgaacttt accgcaggat
     2341 tgacatgagg gttgaccgga tgatggaaga agggcttgaa caggaagctg tttcactgaa
     2401 acagtacagg gaactgaatg ccctaaacac cgtcggttac cgcgaaatgt tcagctggtt
     2461 tgacggagag gtctcacgtg accaggcggt gatgctcatc aagaggaaca caaggcgcta
     2521 tgcacgaaaa cagctcacct ggtggaacag ggataacgac ataaactggt ttgatgtgtc
     2581 ggagcgtgag atgatagtct cctggattga aagccggctg gaggagaact gctcctgaaa
     2641 aactgcctgt acaccattat accggggtag cgcagccggt ctcagaccgg ctgcttcagt
     2701 ttcctgataa cttcagcagg gtctgatgaa gaaaacacgg tattgcctgc aacaagcaca
     2761 tccacaccgg catgcactag catgggagca ttatgcagat ccacaccgcc gtcaacctca
     2821 atcattacgt caaatccacc ctcgtcaatc atgcttctga gttccccgat cttgttgaag
     2881 ctgccgggga tgaaggtctg ccccccgtag ccagggttga cggtcatcag caggaccata
     2941 tcaatatatg gaagaatgtc tttgaggagt atcaccggag agtgaggatt cagtgtcacc
     3001 cccgccttca tcccgagcct gcggatctca gtaacggtcc ggtgaaggtt ccggcacgcc
     3061 tcgaagtgga cagtcaggtt tgaggcacca gcctccctga accgttcaag ataccggtca
     3121 gggtcaacta tcatcaggtg tacgtcaagc ggctttgaag agaggtcgcg gaccgcttcc
     3181 attaccggaa aaccaaaaga tatattggga acaaacagac cgtccattat gtcaaggtga
     3241 agccaatcgg cttcgctgtc attgatcatt tcaacctccg atgcaaggtt ggcaaagtca
     3301 gcggcaagaa gtgacggtgc aacaaggtgg ctcataatta aaagaatatc ggtttggtga
     3361 tcagcccagg tatgacttaa gtatccggct gcgggtggtg aactgaatgc gtttgatggc
     3421 cttttccttg atctgcctga cccgttcacg ggtgagtttc agttcttcgc ctatttcttc
     3481 aagggtgaac gggtgtttca tcccgatgcc gaaataaagc ttcagcaccc tcgcctctct
     3541 gggcgacaga gtgctcaggg ctttttctat ttcataagat aatgattcat tgataaggtt
     3601 acggtcagga ctgggtgttt cttcattctg catcacgtca tacatgttgt tgtcctcttc
     3661 cgaacttatc ggggcatcca tactcagtgt gcgtccgttg gtgcgaatgg cttccttaac
     3721 atcctccggt gctatctcaa gcaggtcagc aatctcctgc gccgaaggct cccgttcata
     3781 ttcctgttca agtttggagt acgccttgtt gatgcggttg atagagccga tcttgttgac
     3841 cggcagcctt acaatccttg actgctcggc aagtgactgc aggatagcct gcctgatcca
     3901 ccagacagcg tatgaaatga atttgaagcc cctggtctcg tcaaaacgct gggcagcctt
     3961 gatgaggccg aggttccctt cgttaatgag gtcagggagt gtcatgccct ggttctggta
     4021 ttgttttgca acggacacaa caaaccgcag gttggctttc acaagccttg ccagggcatc
     4081 atggtcacct gtgcggatac gacgggccag agacacctcc tcatcgggtg agatcagctc
     4141 aaccttcccg atctcctgaa ggtacttatc aagagagggc gtgtcacggt tggtaacctg
     4201 tttggttatc tttaactgac gcattaacaa ttaattagcg gacttactgc cgtttccttt
     4261 acgcagaagc tcacaatttt actaaaatat tctcagataa acaagagttg tgcaactttt
     4321 aatataaatg tcccgtataa agtaattttt taagtattca ccactcgtcg ggtaaaaagg
     4381 tgaaagatca ggaagattcg tgtgccactt tttgtgctgt caatttgtat attaagtaat
     4441 tattaattat acttgcaccg gaataatgac gcaagtcgtt tactcagtcg gagggactct
     4501 ttcatattcc ccgcttcatc aaaaacaatc gaaattaatt cttctacaca ttattacact
     4561 atgcatgata ttcttgaact gagcgagatg ctggttcccg aattgaggga gattgccaag
     4621 cagctcaaaa ttaagagggt agaactactc aaaaagcagg atcttatcta caagatcctt
     4681 gaccagcagg ctattgacgc cgctgagacc cgcaagaccg gcagggccga aaaccagcct
     4741 tctgacagga aacaggagca atccggtcag ccaaacaggc aaggaaagcc tgacagacct
     4801 gcgaagaatc ctgatcagcg tcgccagcag ggtcagcaga accaggggca gcagcaacag
     4861 cagcccaggc agcaaaacct gggacggcct ccgaagccgc agcatgacca gcagcagaga
     4921 aagccccagg gtcagggaca gcagcagggt cagacacagt ctcagccgca gggtcagggt
     4981 cagaaacagg gtcagcaaca gcagcagcag ggtcagcagc atggacagcc acaacctcag
     5041 tcgcagggcc agggtcaatc ccagggtcag aaacagggtc agcagcagca acagggtcag
     5101 cagcatccgc agggacaaca gcagaacagg cagcagcagt ggcagcagaa ccgtgacaag
     5161 cgcccccgca ccttcaggaa tgaccagcag ggaccgtaca ggcatgacca aaatcgccag
     5221 cagcaacaac aacaggcccg tcaggacgaa atggcgcaga caccatcaca gcagccacaa
     5281 caacagtccc agccgcagcc tcagcagcag tcacagcagc agtcacagca gcagccccgt
     5341 gagcaggcct accagaatga acagggactg ccacctgccg acaagcttct ctttaccccc
     5401 gaaacagtta ctgacagcga tcaggatctc ctgaggttcc ctgaacctga gggtgaaagc
     5461 cggatcatcg aggaggcagc cgcacctgag ggtgctgtgg ttatcccgcc ggttccggcc
     5521 gaagccagcg caccccagcc cgaggtgcat gagccggtaa gggaggaaaa gaaagaaaga
     5581 gcttatgatt ttgagggaat agtagcaaat accggtgtgt tggagataat gccagacggt
     5641 tacggtttcc tccgctcacc agactacaac tatctcagtt cacccgatga catttacgta
     5701 tcacaatcgc agataaagct cttcggtctg aagaccggag acaccatcag gggcaccatc
     5761 cgtccgccgc gcgaaggcga aaaatacttc cctctcatca aggtcgaaga gatcaatggc
     5821 cgtacaccgg acttcatccg tgacagggta ccatttgact accttactcc gctgttcccc
     5881 aatgagaaat ttaccctctg caggccgggt gaaggaaacc tgtctgtccg catcgttgac
     5941 atgttctgcc caataggcaa gggacagcgc ggtcttatcg tggcgcagcc aaaaaccggc
     6001 aagaccatcc tgttgcagga tattgccaat gccattgcca aattccaccc ggaggtctac
     6061 ctcatgatcc tgctcatcga cgagcgtccc gaggaagtca ccgagatggc ccgcaatgtg
     6121 aatgcagagg ttattgcatc cacctttgat gagccggcag agaggcatgt gcggatagcg
     6181 accatcgttc aggagaaggc caagaggctg gtggagtgtg gtcatgacgt ggtcatcctg
     6241 cttgactcca tcacccgtct tgcaagggca ttcaacacca tcgccccggc atcgggaaaa
     6301 gtgctctcag gcggtgtcga ctcgaacgcc ctgcacaaac cgaaacgctt cttcggtgca
     6361 gcccgtaaca ttgaaaacgg aggctcactg accatcatcg ccacagcgct cactgacaca
     6421 gggtcgaaga tggacgatgt catctttgaa gagttcaagg ggacgggcaa catggagctc
     6481 cagcttgacc gcaagctctc caacaggagg atcttcccgt cgattgacat accggcttca
     6541 agtacaaggc gtgaggacct gcttcttgac aagaatatgc ttaacaaaat atggatactc
     6601 cgcaactacc tgactgacat gaactcagtg gaggccatgg agttcctgcg tgaccgcatc
     6661 acccagacac gctcaaacga agagttcc
//
LOCUS       DAOPXU010000133         6480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_14608_length_6480_cov_10.623502, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000133 DAOPXU010000000
VERSION     DAOPXU010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14608_length_6480_cov_10.623502"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="PKK03_10880"
     CDS             <1..849
                     /locus_tag="PKK03_10880"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNX84943.1"
                     /translation="ADMTKNVSGVVTDEKGGPLEGAVVLVKGTTAGTTTDGSGRFTLK
                     DVPDDAVLVITYVGYVTKAVSVKAAGNYITTQLQWGNMIIDTVAVPPPPPPPPASGPS
                     KALVVLDGKVTNKPLSEISPDVIAEIHVLKGEQAVAKYGEKGKDGVIEITTKKNTVAS
                     PDKQKEVTVTGYGKEENRDVFVIVEEMPEFPGGMEAMMLWISQRVKYPEQAKKDGITG
                     LVMVSFIVNKAGKVTDISVERSAHPLLDAEAVRVIGEMPAWKPGTQHGKAVEVRMTVP
                     VKFSLR"
     gene            1115..2221
                     /locus_tag="PKK03_10885"
     CDS             1115..2221
                     /locus_tag="PKK03_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HNX84944.1"
                     /translation="MILLNGEVGNYIYSNGKKFSYFAGNNYLGLAGHPEIKKAVIRAV
                     EKYGVNFAASRHTTGTSDLHLELEGALASFKGKEDAVVFASGYQGNSILLEILKGSYS
                     TIFIDQSAHASIIAAIPADVVKVMYYDHCDAGHLDYLLDYNRGSSPLVITDGIFALTG
                     EIAPLNQIYEVVRKHDAILVVDDAHATGVIGATGKGTPEHFGLPGDGKIYQTETMSKA
                     LGGYGGFISGSRSVTGLIREKSATYQASTALPPPIAAAGIASLAIIRENPGLRKELLD
                     KAWTIREALTAMDFRTTHFRTPIVPVLFDTAEKANGLSQFLEENRVIAPSVNYPARQQ
                     LFLVRIAVSATHTDDQISQLLGLLKQWKDKNESN"
     gene            2208..3281
                     /locus_tag="PKK03_10890"
     CDS             2208..3281
                     /locus_tag="PKK03_10890"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNX84945.1"
                     /translation="MKAIKIKKVVIEPLNLDLEEPFRISIGTKFSIENALVTVILDNG
                     IEGYGEAAPLEPINGENQATALATLKSCVDFIKGKDVTEYRSIAATLRGVFQPQVTAR
                     CAIEMALLDAYAKVLGVPLFQFFGGAGNKAETDYTIDIVPPDVARKNAAKLAKEGYTV
                     LKTKVGKKLTDDIDRLLAIKDGAPQCGITIDANQGYSPCEAVHFIEEMTKNGIRPILF
                     EQPVSKYDLAGMRFVKDHTSIPIAADESVFTSADAINVVRTGCADYINIKLMKSGIIE
                     AMDIAAIARSANIKLMIGCMLESKLALGCAVHLVAGMGCFSHVDLDPHSEPEKEPFAG
                     GPDYRAPFYTLSPDLPGIGCTRK"
     gene            complement(3303..5978)
                     /locus_tag="PKK03_10895"
     CDS             complement(3303..5978)
                     /locus_tag="PKK03_10895"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84946.1"
                     /translation="MKLTFIPTILILCCVALSSCSKKDSPIYKSDMYAVYPDRVEQGE
                     FTARALSSEEITSDYQSQSSAIDPVIEFKFSINGRDNELPFGVNHLANIHPVSDRSKV
                     LNIKFGQKSDPVDGSDISDPLPQNTRVRFRVDFSQVLASFREKGYYDDILGNRIFSDD
                     FKGLYIAGDTYPLNWDFENIPGNPDLKLEDPDGDGIFEKELTFNVYDPSAHTSSTWRL
                     TNDISKYPVFGSSSTLLNALHNMALDEMVMLMEEDGTFRTGKEWAGVWTRDVSYATLL
                     SLVWLDPERCINSLMRKVSDNRIIQDTGTGGAWPVSSDRVVWALAAWEIYKYTGDKEW
                     LMNAFGIITTSMEADSMTIPDPGTGLIRGESSFLDWRKQTYPLWMEPADIYGSLNLGT
                     NIAYCQALRVTGMMARELGREDNWSKRAEELEEKINSHLWLEDRGYYGQYLYGRRYRT
                     LSPRAEALGESFSVLFGIADEERSLRVLQNTPVTPFGITCIYPQIPGIPPYHNNGIWP
                     FVQAFWTMANAQERKVEAVEAGIASILRPAALFLTNKENFVAGNGDFEGTETNSDRQL
                     WSVAAMLAMHHRVIMGISFEADRMIFSPVIPESFNDTYSLSGLKYRNGTYNIRVTGWG
                     DGVTSFRIDGNESTDHFIPATMTGSHNIEVVMNGKTTGSTHNAVSNLTAPETPVLSVT
                     GSGLQWSPVSESTGYMVFKNGNMTAATAETEFVPENRDEAAEYQVMASGDDSTESFMS
                     NPVTVSPAGDGIITAEAESFNVRGEDSYPGFSGSGYVKFSLADNRELRIKINAREGNY
                     ALLLRYSNGTGPVNTDNNCAIRSLYVNGIYARALVFPQRGKDEWSDWGYTYPEWIGLK
                     DGENILTIRYEDFNRNMDGEINEFLLDRITLERLN"
     gene            6184..6375
                     /locus_tag="PKK03_10900"
     CDS             6184..6375
                     /locus_tag="PKK03_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNX84947.1"
                     /translation="MSKGIVKFYNEFKGFGFIKEENSPKEYFVHSSGLKDNIKENDVV
                     TFDLEQGKKGLNAVNVKRV"
BASE COUNT         1583 a         1541 c         1712 g         1644 t
ORIGIN      
        1 gcagacatga caaaaaatgt cagcggggtg gttacggatg aaaagggtgg cccgctcgaa
       61 ggggcggtag tattggtgaa gggcacaacg gccggtacaa ccaccgacgg ttcgggacgc
      121 tttacgctta aggatgttcc cgacgatgca gtcctggtaa ttacctatgt cggctatgtt
      181 acgaaagctg tctcggtaaa ggctgccggt aactatatta caacgcagtt gcagtgggga
      241 aatatgatta ttgacactgt agcggttcct ccgcctcctc cgcctccacc agcatctggt
      301 ccgtcaaagg ctcttgtagt attggatgga aaggtgacaa acaaacccct ctcggagata
      361 tctccggacg ttatagcaga aatccatgtc ctgaagggcg aacaggctgt tgcgaaatac
      421 ggtgagaagg ggaaagatgg agtcattgag ataacgacga aaaagaatac tgtcgcttcg
      481 cctgacaagc agaaggaagt aaccgtgaca ggctacggca aggaagagaa cagggatgtt
      541 tttgttattg tggaggaaat gcccgagttt ccggggggca tggaagccat gatgctatgg
      601 atctcccagc gggtgaagta tccggagcag gccaagaagg atggcattac aggactggtg
      661 atggtgtcat tcatagtaaa caaggcaggg aaggttactg acatcagtgt tgaaaggtct
      721 gcacatcctt tgctcgatgc tgaggcggtt agagtgatag gggagatgcc agcctggaaa
      781 cccggtactc aacatggcaa ggctgtcgaa gtgagaatga cagtcccggt gaaattcagc
      841 ctgcggtagg cagtctggta gggttaagca tttgaataat actgatgagg gtatctccct
      901 cagggctggt gagtcatcac cggccctttt ttcttcgggg tacaggaact gtatcatggc
      961 ctgcatatcc cgggaattcc ccgggtcaag ttcagggtgt atgctttacg gcatacaggg
     1021 atcattttcc ggttttttta tgagtggtag gatgtctgat gactatataa aagattaatt
     1081 ttgataaagt cataatttta gttaatcagg gaacatgatc ctattgaacg gcgaagtcgg
     1141 gaattacatt tacagcaacg gcaaaaagtt ttcttacttc gcgggaaaca attacctggg
     1201 gctggccggt catccggaga taaagaaggc cgttatccgg gccgttgaaa agtatggtgt
     1261 caactttgct gcatcgcgtc atactacagg cacatcagac ctgcatcttg agctggaggg
     1321 tgcgctggcc tcattcaagg gaaaagagga tgcagtggtt tttgcatcgg gttaccaggg
     1381 taacagcatc ctgcttgaga tactgaaggg gagctattcg acaatcttta ttgaccagtc
     1441 ggcccatgcc agcatcattg ctgccatccc tgccgatgtg gtcaaagtga tgtattatga
     1501 tcactgtgac gccggccacc tcgattatct gctggattat aaccgggggt cgagtccgct
     1561 ggtgatcact gacgggatct tcgccctgac gggcgaaata gcccctttga accagatata
     1621 cgaggtagtc agaaagcacg atgccatcct ggtcgttgat gatgcgcatg caacaggcgt
     1681 catcggtgcg acgggcaagg gaactcctga gcattttggc cttcccggtg acgggaaaat
     1741 ttaccagacc gagacaatga gcaaggcgct tggcggttac ggcgggttca tatccggcag
     1801 ccgttcggtc accggattga tccgtgaaaa gtcggctact taccaggcct ccaccgctct
     1861 gcctccgccg attgcggctg caggaattgc ttcacttgca atcatcaggg aaaatcccgg
     1921 cctgcgcaag gaactgcttg acaaggcgtg gacgatcagg gaggcattga ctgccatgga
     1981 tttccggaca acccatttca ggactcccat cgtgcctgtt ctcttcgata ccgctgaaaa
     2041 ggcgaatgga ctttctcagt tccttgaaga gaacagggtg atagcgccct ctgtcaacta
     2101 tccggccagg cagcagctgt tcctggtgag gattgctgta tccgccactc atacggatga
     2161 tcagatcagt cagttactcg gtttattaaa acaatggaag gataaaaatg aaagcaatta
     2221 agattaagaa ggtagttatt gagccgctga atcttgatct cgaagagcca ttcaggatat
     2281 ccatcggcac aaagttcagc attgagaatg cactggtgac ggtgatcctg gacaacggaa
     2341 ttgaaggtta cggtgaggct gctccgcttg agccgatcaa cggagagaac caggccacgg
     2401 cgctggccac cctgaagagc tgtgtggact tcatcaaagg gaaggacgta actgaataca
     2461 ggtcgatagc tgccactctt cgcggagtat tccagccgca ggtgactgca cgctgtgcta
     2521 ttgagatggc gcttctcgat gcctatgcca aggtgctcgg ggtaccgctc ttccagttct
     2581 tcggcggagc cgggaacaag gctgagactg attacactat tgatatagtg ccgcccgatg
     2641 tggcaaggaa gaatgccgcc aaactggcga aggagggata taccgtgctg aaaacgaagg
     2701 tgggaaagaa gcttacggat gatattgacc gtctgcttgc aatcaaggat ggggctccgc
     2761 aatgcggcat cacaattgac gccaaccagg gatattcacc atgcgaagcg gttcacttta
     2821 ttgaggagat gacaaagaat ggcataagac ccatcctgtt cgagcagcct gtctcaaagt
     2881 atgaccttgc cgggatgagg ttcgtgaaag accacacttc gatacctatt gccgcagacg
     2941 agtcggtatt cacaagcgct gacgccatca atgttgtacg gaccggatgc gcagactata
     3001 tcaatatcaa gctgatgaag tcaggcatca ttgaggcgat ggacattgca gccattgcca
     3061 gaagtgcaaa catcaaactg atgatcggct gcatgcttga atcaaagctg gccctgggtt
     3121 gtgcagtgca cctggtggcc gggatgggct gtttcagcca tgttgatctc gatccgcaca
     3181 gcgagcctga aaaggaaccc tttgcaggcg gacctgatta cagggcaccg ttctatactc
     3241 tttcaccgga tcttcccggg atcgggtgca ccaggaaata gtcgccggca aaccgctgcc
     3301 cgtcagttga gcctctccag agttatcctg tcgaggagaa attcgtttat ctccccgtcc
     3361 atgttcctgt tgaagtcttc gtacctgatg gtaaggatgt tttccccatc cttcaacccg
     3421 atccactcgg ggtatgtgta gccccagtcc gaccattcgt cctttccgcg ctgagggaat
     3481 acaagcgccc tggcatagat gccattgaca tacagtgacc gtatcgcaca gttgttgtcg
     3541 gtgttgacag gtccggtccc gttggagtag cgcaacagca gtgcataatt cccttccctg
     3601 gcgttgattt tgatcctgag ttcacggttg tcagccaggc tgaacttcac atacccgctg
     3661 ccgctgaatc ccgggtaact gtcttcaccg cggacattga agctctctgc ttcggctgtt
     3721 atgatgccgt ctcccgccgg tgagacggtg acagggttgc tcatgaagga ctctgttgag
     3781 tcgtctcctg aagccattac ctggtattca gccgcctcat cccggttctc aggaacaaat
     3841 tctgtctctg cagtggcagc agtcatatta ccgtttttga aaaccatgta gccggttgat
     3901 tcgctcaccg ggctccactg aagaccagag cctgtcactg aaaggaccgg tgtctccggg
     3961 gcggtcaggt tactgacagc attatgggtg ctgccggtcg tctttccgtt catgactacc
     4021 tcaatattat ggctgcctgt cattgtggcc gggataaaat gatcagtcga ttcgtttccg
     4081 tcaatcctga atgatgtgac tccgtcaccc cagcccgtga cccggatatt ataggttccg
     4141 ttacggtatt tcaggccgga cagcgagtac gtgtcgttga aactttccgg aataaccggg
     4201 ctgaaaatca tcctgtctgc ttcgaagctt atccccatta tcacacggtg gtgcattgcc
     4261 agcattgccg caacgctcca caactgcctg tcagagttcg tttcagttcc ttcgaaatct
     4321 ccgttcccgg ccacgaagtt ctccttattg gtaaggaaga gtgcagcagg gcgtagtatc
     4381 gaggctatgc cggcctcaac cgcttcaact tttcgctcct gtgcattggc catggtccag
     4441 aaagcctgca cgaagggcca tatcccgttg ttgtgatatg gaggtatccc gggtatctgc
     4501 ggatagatgc atgtaatccc gaaaggggtg acgggagtgt tctgaagcac cctgaggctc
     4561 ctctcctcat ctgcgatgcc gaagagtacc gaaaatgatt cgccgagggc ttccgcccgg
     4621 ggagaaagtg tcctgtagcg tcttccgtaa aggtactggc cgtaatagcc cctgtcttcc
     4681 agccagaggt ggctgttgat cttttcctcg agttcttcgg cccttttgct ccagttgtct
     4741 tctctgccta gttcccgggc catcatccct gtcactctga gtgcctggca gtaggctatg
     4801 ttggtgccca ggttgaggga gccgtaaata tctgcaggtt ccatccacag cggataggtc
     4861 tgcttcctcc agtcgaggaa ggatgactca ccccggatca gtcctgtccc tggatcaggg
     4921 attgtcatgc tgtcagcttc catgctggtt gtgataatcc cgaaggcatt catcagccac
     4981 tctttgtctc cggtatattt atatatctcc catgcagcca gcgcccatac caccctgtca
     5041 gaagaaaccg gccatgctcc accggtgccg gtatcctgta ttatcctgtt atcagacacc
     5101 ttgcgcatga gactgtttat gcatctttcc gggtcaagcc agaccagtga gagtagggtg
     5161 gcatagctca catcgcgggt ccagaccccc gcccattctt taccggtacg gaatgtcccg
     5221 tcctcttcca tgagcataac catctcgtca agtgccatgt tatgaagggc attgagaagg
     5281 gtggaggagc tgccaaacac cggatatttt gagatatcat tggtcagtct ccaggtggat
     5341 gatgtatgtg ctgaaggatc atatacattg aatgtcagct ctttctcgaa gatgccgtcg
     5401 ccgtccgggt cttccagctt caggtcaggg tttcccggaa tattttcaaa atcccagttg
     5461 agcgggtagg tgtcacccgc aatgtaaagt cccttgaaat cgtcactgaa tatccggttc
     5521 ccgagaatat catcatagta tcccttttct ctgaaagagg ccagtacctg gctgaagtcg
     5581 accctgaatc tgaccctggt attttgcgga agaggatctg agatatctga accgtcaaca
     5641 gggtctgact tttgtccgaa cttgatattc agtactttgc ttctgtcact gaccgggtgg
     5701 atatttgcca ggtgattgac tccgaaaggc agctcattgt cacgtccatt gatgctgaac
     5761 ttgaattcta tgaccgggtc aatagcggag ctctggctct gatagtcaga ggtgatttct
     5821 tcagagctca aagcgcgggc tgtaaattca ccctgctcca cgcggtcagg gtaaaccgca
     5881 tacatgtctg atttatagat gggagaatct ttcttgctgc acgatgaaag tgccacgcaa
     5941 cagagtatta aaatagttgg aatgaaagtc agtttcatat caattttgtc aatgttcggt
     6001 tatgtccagt ctttcaaatt taatgaatgc gggggatcta tccctgcaat aatccacact
     6061 ccaaaagtgt atctgattaa ctgctttttt gtttcttttg cgacatcagg tataatttat
     6121 tgaaaataaa taaaatatat agtttttaag ttgtatattg ctgcatatta ataaaatcaa
     6181 ataatgagta aaggaatagt aaaattctac aatgaattca agggattcgg atttatcaaa
     6241 gaggagaatt ccccaaagga gtattttgtc cattcatctg gtctgaagga caatataaag
     6301 gaaaatgatg ttgttacttt tgacctggag caggggaaga aaggattgaa tgcagtaaat
     6361 gtcaaacgag tatagtttta gtccaattac ttgattatca agagtcccac cggagtcggc
     6421 gggacttttt attgcaatta aggaaatcat gatagaaaag aaaccagtaa ttcacaactc
//
LOCUS       DAOPXU010000134         6429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_14784_length_6429_cov_12.770003, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000134 DAOPXU010000000
VERSION     DAOPXU010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14784_length_6429_cov_12.770003"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..886)
                     /locus_tag="PKK03_10905"
     CDS             complement(50..886)
                     /locus_tag="PKK03_10905"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNX84948.1"
                     /translation="MERMKILVIGASGLLAGPVIRKLDERGYDLRLFSRSVKPSMFVN
                     DYEIAQGDVFNVADLEKAVTGCDAVHISLASADDVKAVSNILEVSKRNGVRKISLISG
                     CTVAEENRWFKFTDDKFRTEQMIMQSGLSWYIFRPTWFFESLPMFVRNGKASVLGRLT
                     DRYHWVAADDLGTMVADAYSTAGAENRFYYVYGPERYTMKEMLEKYCSALHPEIKSVG
                     ETPLLPLKIIAFLTRNRQLKFAASLFGYFEKVHEPDFSEADLALLTRPATDFDTWVRS
                     KQ"
     gene            complement(970..1533)
                     /locus_tag="PKK03_10910"
     CDS             complement(970..1533)
                     /locus_tag="PKK03_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNX84949.1"
                     /translation="MDFKDRLIEHYGFTRRDWNETVSRYRPETLQARSLFLEKGQIAT
                     RIAYLRSGMMRSFFYDDRANEITTHFFKKGSVVISMKSFNDQVPSREYIIAIEDCEMD
                     VITYDRMQELIRVVPAWRQIGKDVDEAMYNSLMNRSIQLQTLTASERYELFIKKHPDI
                     LQKVALKHIASYLGIDIATLSRIRKSI"
     gene            1700..2908
                     /locus_tag="PKK03_10915"
     CDS             1700..2908
                     /locus_tag="PKK03_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX84950.1"
                     /translation="MKEYIERPLYIDRIKPYIGKSLIKVITGQRRVGKSCMLMQVRDL
                     IMEKDHDTHIIFINKEQHEFAPITDHVTLLEYVGLHSKGKGKVALFIDEIQEIDSFEV
                     ALRDLSTRKKFDIYCTGSNARLLSGELATLLAGRYIEIRVYGLSYSEYLMFRDAADSP
                     DSFRTYLREGGLPYLIHLGTDINVVNEYLNTIYSTILLKDVVARYNVRNVKFLESLVL
                     FLADNTGSIVSAKKISEYLKSQNINISTQVVIDYIGYLESSFMIHRVRRTGIRGKRLF
                     EIGEKYFFGDTGIRNVIAGYNLADVHKMLENIVFLHLRTAGYEVTVGSENGKEIDFIA
                     SRGGERIYVQVAYLLEKPETAQREFGNLLGISDNYPKYVVTMDELNETSSYQGIQRMH
                     VKDFCRMMAG"
     gene            complement(2949..4652)
                     /locus_tag="PKK03_10920"
     CDS             complement(2949..4652)
                     /locus_tag="PKK03_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84951.1"
                     /translation="MNQGIPISDFEQFVDEKLLARGLSYFENGKVTSFEELSPGEFEA
                     EVAGTEDYDVRITVSRRKITSYLCTCPYDYGTVCKHIVAVMYYIRNEEEKLKKADNKK
                     GSARKPTSGRKTVSEQVDELLGALSHDDLNSFIKEQCLADPAFRRSFLSGFMYKSSAE
                     SPEKYRDQVKNILRSARDKDRFIKWGRAGIVGRSVNEMLVIALKHVDSGNWLSACHIC
                     FPVAEEMVKALQFADDSDGDIGGNIETAFEILYSVAASDRPEEIRAYMLDQVLHYYRK
                     NVFSGWDWHTDLLRLAVDLVKTEKEAGTVLSLLHTHVHSEYEGEQMTMLEYELIRKMK
                     GDDEAEKFASQNLDTPELRRKALEKVLRDKDFEKAKSLAHAGIKNDAETKPGLAKEWY
                     DWLLRTAVAEKDKPHVIEYARYLFVDGFRNDQDYYQLLKKNIDPVEWNSYVEGLVDEI
                     RRRYRWDNISVLGKIYITEKWWDRLLDLVRGAKHLPYIQHYEKYLAADYSEELAELYE
                     KGIIDYLKVNTGRKHYQEACRYLERMLKLGGLARVRNLIADLRREYPQRRALLDELDK
                     F"
     gene            complement(4664..>6429)
                     /locus_tag="PKK03_10925"
     CDS             complement(4664..>6429)
                     /locus_tag="PKK03_10925"
                     /inference="COORDINATES: protein motif:HMM:NF021375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HNX84952.1"
                     /translation="EFVSWQNSLSRVRDLLELGEIENSFVALEYEVPYNQSRLDCLLF
                     GKGEGDSSNIVLIELKQWTSVTALPEEGNFVETYTGGRERVVPHPSQQAKGYHYYLTG
                     FVEEFEKTPTLNLFSCAYCHNYSRSDGKGLFNPVYSELIRDFPLYTKEDTKVLGARIK
                     ELLDKGSGLEIFNRFMQSRIHPSKKLLENVHKVIKNDAVFSLLNEQLVAKNLIWAHVR
                     KTSRDGSKSVIIVHGGPGTGKSVIAINLLAEAALRKKNVLFGCKSKPFTNGLQKLVGR
                     GAELMFTNLYRFVPSRTAENEFDILLIDEAHRIEKSSNHQYTRPADRTDMPQINQLIR
                     CAKTSVFFIDDRQKVRSQETGNSDLIRNAAIVAGATVREVTLETQYRCMGSNDYLLWI
                     ESVLGYSEEKRILLRNEIFDFRIMSSPSEIMEILKEKEAEKANSARMVAGFCWPWSSK
                     TDANGDLIKDVRIGDFAMPWETHDKITRTPPGYVKWYEWAYRPEGIKQVGCIYTAQGF
                     EFDYIGVIIGNDLRYDPHTDSLTGDITASKDPMLKRDFQTFDMHVKNIYRTLLTRGMK
                     GCYVYFTDKGTEDFFRNRMES"
BASE COUNT         1580 a         1557 c         1509 g         1783 t
ORIGIN      
        1 tcgccggatc ggtcaccccg tcccgtgagt tgttcttttt ctccatgatc tattgctttg
       61 acctgaccca ggtgtcaaaa tcagtcgccg gcctggtcag cagggcgagg tcagcttcgg
      121 aaaaatccgg ctcatgcacc ttttcgaaat acccgaacag cgaggctgca aacttcagct
      181 gcctgttgcg ggtcaggaag gcgatgatct tcaggggcag gaggggagtc tcaccgacac
      241 tctttatctc cggatggagg gcgctgcagt atttctcaag catctccttc atcgtgtatc
      301 tctccgggcc gtagacataa tagaacctgt tctctgcccc ggcggttgaa tatgcatctg
      361 ccaccattgt gcccaggtcg tcagcagcca cccagtgata ccggtctgtc agcctgccca
      421 ggacagaagc cttgccgttc cggacgaaca tgggcagcga ctcgaagaac catgtaggcc
      481 ggaagatata ccacgaaaga ccgctctgca tgatcatctg ttccgtcctg aacttgtcgt
      541 ccgtgaactt aaaccaccgg ttctcctccg ccacggtgca tcccgagatc aggctgatct
      601 tcctgacacc gttcctcttc gagacttcga gaatgttcga gaccgctttc acatcgtccg
      661 ccgaggcgag tgaaatatgc acagcatcgc acccggtcac tgccttctca aggtctgcca
      721 cgttgaatac atcgccctgt gcgatctcat agtcattgac gaacatcgac ggtttcaccg
      781 accgcgagaa gagtcgcagg tcataccctc tttcgtccag tttgcggata acgggacccg
      841 ccagcagccc gctggcgccg ataacaagaa tcttcatcct ttccatagct gttgttgttt
      901 gatttgctga tgaggcaaag ttaccccacc ggtttgggag cgttgtttga caaaagtcaa
      961 aaaaagggct cagatcgatt ttctaatcct gctgagggtg gcgatgtcga tgccgaggta
     1021 ggatgcgata tgtttcagtg cgactttctg caggatgtca gggtgttttt tgatgaagag
     1081 ctcgtaccgt tccgatgctg tgagggtctg gagctgtatt gaacggttca tgagggagtt
     1141 gtacattgcc tcgtccacgt ctttgcctat ctgccgccat gccggcacca cgcgtatcag
     1201 ctcctgcatc cggtcataag tgatcacgtc catctcgcag tcctcgatgg cgattatgta
     1261 ttcccttgac ggcacctggt cattgaagct tttcatcgag atcaccaccg agcccttttt
     1321 gaagaagtgg gttgtgatct cgtttgccct gtcgtcatag aagaatgacc gcatcattcc
     1381 cgaccgcagg taggcgattc gtgtagcaat ttgtcccttc tccaggaaca gtgatctggc
     1441 ctgcagtgtc tccggccggt agcgactgac ggtctcattc cagtccctcc tggtaaaacc
     1501 gtaatgctca atcaacctgt ctttgaagtc catagtagag taaagataca gttgttggat
     1561 ttcatggcaa agcagtcgac aggtttcaaa atggtataaa tgaacagcat gattgacgaa
     1621 tttaaataaa tagcattaaa cataagatat aattgacgaa aggtattata tttgtaaaag
     1681 cataagataa gattgaagaa tgaaagaata cattgagagg cctttgtata ttgacagaat
     1741 aaagccttat atcggcaaga gtctgataaa ggtaattacc ggccagagga gggtgggtaa
     1801 gagctgcatg ctgatgcagg taagggatct cataatggag aaagaccacg acacccacat
     1861 tattttcatt aataaggagc agcatgagtt cgccccgatc actgaccatg tcacgcttct
     1921 tgaatatgtt ggccttcatt caaagggaaa agggaaggtt gcgttattca ttgatgagat
     1981 ccaggagatt gattcatttg aagtagcgtt gagagacctg tccacaagga agaagtttga
     2041 catctactgt accggtagta acgcaaggct gctttcaggt gagctggcaa ctcttctggc
     2101 ggggcgttac attgagataa gggtgtatgg cctgagttat tccgaatacc tgatgtttcg
     2161 cgatgctgct gattcaccgg attcattcag gacatacctg cgtgaaggag ggcttcccta
     2221 tctgatccat cttggaactg acataaatgt tgtcaatgag tatcttaata ctatttatag
     2281 caccattctg ctgaaggatg tggtggcaag gtataatgtg cggaatgtta aatttcttga
     2341 gagtctggtt ctttttcttg ccgataatac aggaagcatc gtttccgcaa aaaaaatcag
     2401 cgaatatctg aaatcacaga acataaatat ttcaacacag gtagtgatag attatatcgg
     2461 ataccttgaa tcctctttca tgatacacag ggtgaggcga acaggtatcc gggggaagag
     2521 actctttgaa ataggagaga aatacttctt cggtgacacc ggaatcagga atgtcattgc
     2581 aggatataat ctggctgatg ttcataaaat gctggagaac atcgtttttc ttcatctcag
     2641 gactgccggc tatgaggtga cggttggatc ggaaaacggc aaggaaattg acttcatcgc
     2701 cagtcgggga ggagagcgga tttatgttca ggttgcatat cttcttgaaa agcctgagac
     2761 ggcacagcgt gaattcggaa atctgcttgg gatcagcgac aattacccca aatatgtggt
     2821 aacgatggat gagctgaacg aaacctcatc ttatcagggc atacagagaa tgcacgtgaa
     2881 ggatttctgc cggatgatgg caggttgact acacacaggc gacccgcgat gcaggccgtt
     2941 gtatgcgttc aaaacttgtc aagctcatcc agcaatgccc ttctttgagg gtattctctg
     3001 cgcaagtcag caataagatt cctgaccctg gcaagacctc ccagtttaag catccgctcc
     3061 aggtaccggc aagcttcctg ataatgcttt ctgcccgtat ttactttcaa ataatcgatg
     3121 atgcctttct cataaagctc tgcaagttcc tcagaataat cggctgccag atatttctca
     3181 taatgctgga tgtaaggaag gtgtttggcg ccgcggacaa gatccagaag cctgtcccac
     3241 catttttcgg ttatgtaaat ctttcccagg acactaatat tgtcccaccg gtatcttcgt
     3301 ctgatctcat cgaccagccc ttccacatag ctgttccatt caacgggatc aatgtttttc
     3361 ttcaggagct ggtagtaatc ctgatcatta cggaacccgt caacaaaaag atatcgggca
     3421 tattcaatca catgtggttt atccttttca gcaactgctg tacgcagaag ccagtcgtac
     3481 cactcttttg caagtcccgg tttggtttct gcatcgtttt tgataccggc gtgagcaagt
     3541 gatttggctt tttcgaaatc cttatccctc agaactttct ccagtgcttt tctcctgagc
     3601 tcaggagtat caagattttg ggaggcaaat ttttcagcct cgtcatctcc cttcattttc
     3661 cggataagtt catattccag cattgtcatc tgttcgccct catattctga atgcacatgt
     3721 gtatgaagca gggagaggac tgtgccggct tccttttccg tttttaccag gtctaccgcg
     3781 agacggagaa gatctgtatg ccagtcccaa cctgaaaaaa catttttcct gtagtaatga
     3841 agcacctgat caagcatata agctcttatt tcttcaggtc tgtcagatgc agccacagaa
     3901 taaagtatct caaaagcggt ttcgatattc ccgccaatgt ctccgtcgct gtcgtctgca
     3961 aactgaagcg ctttcaccat ttcctcagct accgggaaac agatatggca tgcgctgagc
     4021 cagttgcctg aatcaacatg tttcagggca atgacgagca tctcgttcac ggatctccct
     4081 acaattccgg cacggcccca cttaatgaac ctgtctttat ccctggcact ccttaaaata
     4141 ttcttaacct gatcccggta tttttccgga gattcagctg aagatttgta cataaagccg
     4201 gaaaggaatg atcgcctgaa tgccgggtct gccagacatt gttctttgat aaaagagtta
     4261 agatcgtcat gtgaaagagc cccaagcagt tcatctacct gttcagaaac agtttttctt
     4321 ccggatgttg gtttccttgc cgaacctttt ttgttgtcag ccttttttag tttctcctcc
     4381 tcatttctga tataatacat tactgcaacg atgtgcttgc agaccgtccc gtagtcgtat
     4441 ggacaggtac agagataaga ggtaattttt ctcctggaaa ctgttatcct gacatcataa
     4501 tcttctgttc ctgccacctc ggcttcaaat tcccccggcg agagctcttc aaaagaggtc
     4561 accttcccgt tctcgaaata agacaagccg cgggcgagaa gtttttcatc cacaaattgt
     4621 tcaaaatcag agatagggat gccttgattc atagtctgca tatttaagat tccatcctgt
     4681 ttctgaagaa atcctcagta cccttgtcgg taaaatagac atagcatccc ttcatcccgc
     4741 gggtcagcag tgtgcggtag atgtttttga catgcatgtc aaacgtctga aaatcacgtt
     4801 tcagcatggg gtcctttgaa gcagtgatat caccggtcaa cgaatcagtg tggggatcat
     4861 accgcaggtc attgccaata atgactccga tatagtcaaa ttcaaatccc tgggcagtgt
     4921 aaatacatcc aacctgttta atgccctccg gcctgtaagc ccattcatac catttaacgt
     4981 aacccggagg tgtgcgtgtg atcttgtcat gtgtctccca tggcattgca aagtcaccga
     5041 tcctgacatc cttgatcagg tcaccgtttg catctgtctt gctgctccag ggccagcaga
     5101 atcctgcaac catacgcgcc gagttagcct tctcagcttc cttctccttc agtatctcca
     5161 ttatttccga aggcgatgac attatcctga agtcaaatat ctcattcctg agcaggatac
     5221 gtttttcctc cgaataaccc aggaccgatt ctatccacag caggtagtca tttgaaccca
     5281 tgcacctgta ctgtgtctca agggtcactt ccctgacggt ggctccggct acgatggcag
     5341 cattcctgat aaggtcggag ttgccggtct cctgtgatct caccttctgc ctgtcatcaa
     5401 taaagaatac tgatgtcttt gcacatctta tcagctggtt gatctgcggc atgtccgtcc
     5461 tgtccgcagg acgggtatac tggtggttgc tgctcttttc aatcctgtgt gcctcgtcaa
     5521 taaggagaat atcaaactca ttctctgcag tacggcttgg tacaaacctg taaaggttgg
     5581 tgaacatgag ttcggcgcca cggccgacga gcttctgaag cccgttggtg aatggttttg
     5641 atttacaacc gaacaaaaca ttcttctttc ttaatgctgc ttctgcgaga aggtttatgg
     5701 ctataaccga tttgccggta ccggggccgc cgtgcactat gatgacggac ttcgaaccgt
     5761 ccctgctggt tttcctgacg tgagcccata tcaggttctt ggccacaagc tgttcattta
     5821 gcagggagaa gaccgcatca ttttttatca ccttatgtac attttcaagc agctttttgg
     5881 agggatgtat ccggctctgc atgaagcgat tgaaaatctc aaggccggac cctttgtcaa
     5941 gcagctcctt tatccttgca cccaggacct tggtgtcttc ctttgtatag aggggaaagt
     6001 ccctgataag ttcagaatag acagggttaa ataacccctt tccgtctgat cgcgaatagt
     6061 tatggcaata ggcgcagctg aagaggttaa gtgttggtgt tttctcaaat tcctcgacaa
     6121 acccggtaag gtagtaatgg tatcctttag cctgctggga agggtggggg acaacccgtt
     6181 ccctgccacc ggtataggtt tcaacaaagt taccctcctc tggcagtgcg gtgacggaag
     6241 tccattgttt gagctcgatg aggacaatat tggaggagtc gccttcccct tttccgaaca
     6301 ggaggcagtc gaggcggctc tggttatatg gcacctcata ttcgagagca acgaaggagt
     6361 tttctatctc accgagttca agcaggtctc tgactctgga aaggctgttc tgccaggata
     6421 caaattcct
//
LOCUS       DAOPXU010000135         6301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_15243_length_6301_cov_11.885046, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000135 DAOPXU010000000
VERSION     DAOPXU010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15243_length_6301_cov_11.885046"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PKK03_10930"
     CDS             <1..816
                     /locus_tag="PKK03_10930"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNX84953.1"
                     /translation="ETVIKIKDMVDRLASSFRLGNAIRKGIPVVIAGRPNVGKSSLLN
                     ILLQEERAIVSDIPGTTRDTIEEVLHLGGLLFRFIDTAGLREGGDEIEELGMERTRSK
                     LRAAEVVLAVAEATDTDESIAALAASIGEITGNSGARLIVLVNKSDLITRKRIAELKR
                     ELKDICPCPALFVSASQSEGIEQVRKQLIASVETGKLDNPQYIVTNARHYEALKNVSD
                     SLGRVLDGFRDGIPTVLIATDVRQSIYYLGQITGRITPDDILGEIFSKFCIGK"
     gene            complement(892..2016)
                     /locus_tag="PKK03_10935"
     CDS             complement(892..2016)
                     /locus_tag="PKK03_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNX84954.1"
                     /translation="MIRDFRKKDIEEVFRTPIVQQTAFWSEVKRRMGISSIACNFRVP
                     GSEINGNGSDAVNITSDVLVVLRQIDPHHSIAYVPYGPELEPLDEYRGIFLEELSECL
                     RSQLPGDCIMVRYDLAWESFWAREDDAYTPEGIWLGPPSQQSQELRFNYGSFHHNLKK
                     AHTNILPSNTIFLDLRKGEESLLQSMKSKTRYNIGLSQRNGVTVSVAGTEDLSRWYAL
                     YTETARRNGFFLHNIEHFRALLTSTAENSQSPAEVFLLVAEHDNEPLAAMFLVITGNR
                     GTYLYGASSSRKRNLMATYALQWKAILLAKERGCTEYDMFGIAPNPDPDHPMYGLYRF
                     KTGFGGEIYRSMGCWDYPLDRAAYSYFAATELTSQGYHIT"
     gene            complement(2144..3421)
                     /gene="aroA"
                     /locus_tag="PKK03_10940"
     CDS             complement(2144..3421)
                     /gene="aroA"
                     /locus_tag="PKK03_10940"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNX84955.1"
                     /translation="MPKKTYFNLMNNTVNPSGLAGLTDAPPSKSLTQRAIAAGLLSGG
                     TTVIRNASFCNDSMAAINMAQALGATIMQQEERINIIAGIRPAGPVTLNCGESGLALR
                     MFAPVAGLLSESVTFTGEGSLVRRPVTMIREALIQLGVKVETSGGLLPLKLSGRLRPG
                     RAFIDGSSGSQLLTGLLMALPMADGDSRIDVASLTSKPYIGLTLGLLAEFGIKVENDD
                     YRSFIIPGRQSYRAHEYTVEGDWSGAAFLLVAGATAGRVTVDGLNPCSLQADRAVLQA
                     LEEAGAGLITGPCGITASRSELKAFTFEATDAPDLFPPLAALAARCEGTSRITGVGRL
                     RHKESDRAEAIAEVLGAMGIRVRFSGNEMLITGGQAKGATVSSHNDHRIAMMAAVMAA
                     SAAGPVTIAGAEAVSKSYPGFYDDLTRLGAVIS"
     gene            complement(3437..4564)
                     /locus_tag="PKK03_10945"
     CDS             complement(3437..4564)
                     /locus_tag="PKK03_10945"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNX84956.1"
                     /translation="MKTDDLTMIAPGIRNRLNVVPVEEWPLFKKRPLVIAGPCSAESE
                     EQVLATAAALSASGRVDLFRAGIWKPRTRPGQFEGVGSPGLRWLRRVKDEYGLPVAVE
                     VATERHVYEALKHGVDAVWIGARTSVNPFAMHEIASALKDSDVMVFVKNPVNPDLELW
                     IGALERISRAGITRIAAVHRGFSTWEKCSFRNQPRWQIAVELRQRIPGIGLLCDPSHM
                     GGDRDFIGDLSQKAMDLHYDGLMIESHINPAGALSDRKQQITPVELMKVLGSLVLRSP
                     TTDDPEFLETIEDLRSQIDMYDNLLLDILESRMQVAETIGRYKKEHGITILQSSRWRE
                     VKDNAVDKGVRKGLTPEFIQAVLEAIHQESITHQMKVMKEG"
     gene            complement(4580..5431)
                     /locus_tag="PKK03_10950"
     CDS             complement(4580..5431)
                     /locus_tag="PKK03_10950"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HNX84957.1"
                     /translation="MSLNICITGLGLIGGSLARSLRLNGFTGRIIGVEANPHHCTIAM
                     YRGLADEFLPLEEAVAKSDIIVLAAPVDVNCRLLPGILDIVTGTGKVVTDMGSTKADI
                     AAVADRHPARGRFVAAHPMAGTEHSGPAAAVDHLFSDRVAIICDPDRSDEDAMEKITA
                     LLQSLNMKIQYMDSREHDIHVAYVSHISHITSFALALCVLDKEKDAANILSLAAGGFE
                     STVRLAKSSGETWAPIFTGNAGPILEVIDTYIDKMLLFRQHIGSGNGEALRQLMKNAN
                     SIGKILK"
     gene            complement(5438..>6301)
                     /locus_tag="PKK03_10955"
     CDS             complement(5438..>6301)
                     /locus_tag="PKK03_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNX84958.1"
                     /translation="MHISMAFLDPGDEVLVPDPGYPAYASAAKLAGGVVRSYDLSEEN
                     GWRPDLRAIETSGTDRVKIMWINYPHMPTGARASYTLFEELVRFARRNRILLCNDNPY
                     SFILNNDHLSILSVPGADETALELNSLSKSHNMAGWRIGMLAGNSTYINNVLKVKSNM
                     DSGMFQPLQVAAAAALALPESWYEELNSVYSRRRILAAQIMNALMCTFDDSQSGLFLW
                     GKIPDGYNDAEELTEELLHNNHLFVTPGSIFGNNGRRHIRISLCASEELLSGALIRVR
                     ERVNTNQLKTA"
BASE COUNT         1396 a         1741 c         1650 g         1514 t
ORIGIN      
        1 gagacggtga taaaaatcaa ggacatggta gacaggcttg cctcctcgtt ccggctgggc
       61 aatgccatca ggaagggaat cccggtggtg atagcaggga ggccaaatgt tggcaagtct
      121 tcgctcctga acatcctgct tcaggaggag cgtgccattg tctctgatat tccgggcact
      181 acccgtgata caattgaaga ggtacttcac cttggcggac tgctgttcag gttcattgac
      241 actgccgggc tcagggaagg cggtgatgag attgaggaac tgggaatgga gagaacccgc
      301 agcaagctca gggccgcaga agtggtgctt gctgttgccg aggccactga cacagatgag
      361 agcattgccg ccctggctgc cagtataggt gagattacgg gcaacagcgg ggcgcggtta
      421 atcgtgctgg tcaataaatc tgacctgatc accaggaaaa ggattgctga actgaagagg
      481 gagctgaagg atatctgccc ctgccctgcc ctgtttgttt cagccagcca atcagaagga
      541 attgagcagg taaggaagca gctgattgca tcggttgaga ccgggaagct tgacaatccg
      601 caatacattg ttaccaatgc caggcattac gaggcgctga agaatgtttc tgacagcctc
      661 ggaagggtgc ttgacggatt ccgtgatggc atccctaccg tgttgatagc cacagatgtc
      721 cgtcagtcca tctactatct gggtcagata accggccgta tcacccccga tgatatcctc
      781 ggggagatat tctcaaagtt ttgtatcgga aaataatttt accatgttct accgcgacct
      841 gacattgcgg gctttggctt gcagtcatct ggcgccagtg tggtgaacca gtcatgttat
      901 atgatacccc tggcttgtca gctctgtggc agcaaagtaa gaataggcag ccctgtcaag
      961 tgggtaatcc cagcagccca tcgagcggta gatctcaccc ccgaagccag ttttaaaacg
     1021 gtagagtccg tacatagggt ggtcagggtc aggattggga gctatgccga acatgtcata
     1081 ctcggtacag cctctctcct ttgccagtaa aattgctttc cactgaaggg cataggtggc
     1141 catcaggttg cgcttgcgtg atgatgaggc gccataaaga tatgtgcccc tgttgccggt
     1201 tatcacgagg aacatggctg ccagcggttc attgtcatgc tccgcgacaa gaagaaaaac
     1261 ctcggcaggc gactgtgaat tctctgcggt gctggtgagc agtgccctga aatgctcaat
     1321 gttgtggagg aaaaaaccgt tcctgcgcgc cgtttcagta tacagggcat accacctgga
     1381 gagatcctcc gtgcctgcca cgctgacagt gacaccgtta cgctgagaaa gcccgatgtt
     1441 gtacctggtc tttgacttca tcgactgcag cagtgattct tcgcccttcc tgaggtcaag
     1501 gaatatggta tttgaaggca atatattggt gtgggccttc ttcaggttat gatggaagct
     1561 tccgtaattg aatctcagtt cctgggactg ctgtgacggc ggtcccagcc agattccttc
     1621 cggtgtatat gcatcatcct ccctggccca gaacgactcc catgcaagat catacctgac
     1681 catgatgcaa tcacccggaa gctgtgagcg gaggcactct gagagctcct ccaggaatat
     1741 gcccctgtat tcgtcaaggg gctcaagctc cggaccgtaa gggacataag caatggagtg
     1801 gtgcggatcg atctgcctca gtacaaccag gacatccgag gtgatattga cagcatcact
     1861 tccgtttcca ttaatctcag aaccgggtac cctgaagtta caggctatcg aactgatgcc
     1921 catacgccgc tttacctcag accagaatgc cgtctgctgc actatgggtg tcctgaatac
     1981 ctcttctatg tctttctttc tgaaatccct gatcatgatc gtctgcctgc gataacttgc
     2041 ttcagttgct gaaggcgtgg caaacatggg acttaataat cagaattcca aggatttttg
     2101 tctctgacag aataacagga tccggacagg ttgttctcct gagtcaggaa attactgctc
     2161 caagcctggt caggtcatcg tagaagccgg ggtatgactt gctgactgcc tccgctccgg
     2221 cgatagtaac gggacctgcg gcgctggcgg ccatcacagc ggccatcatg gctattcggt
     2281 ggtcgttgtg tgatgagacg gtggcgccct tcgcctgccc gccggtgatc agcatctcgt
     2341 taccgctgaa ccgcacgcgg atgcccattg cgccgagcac ctccgcgatc gcctccgccc
     2401 tgtcgctctc cttgtgcctc agcctgccca ctccagtgat cctgctcgtc ccctcacatc
     2461 gggccgccag cgccgccagc gggggaaaca gatcaggtgc gtcggtggcc tcaaaggtga
     2521 atgccttcag ctccgaccgg ctggcggtaa ttccgcatgg acccgtgatc aggccagccc
     2581 ctgcctcctc aagagcctga agcactgctc tgtcagcctg caggctgcag ggattaagcc
     2641 cgtcaacagt caccctgcct gccgtggcgc cggcaaccag cagaaaagcc gccccgctcc
     2701 agtcgccctc aacggtgtac tcatgcgccc tgtacgactg tctcccgggt atgataaatg
     2761 acctgtaatc atcattctcc accttaatgc caaattcggc aagcagcccc agggtaagtc
     2821 cgatgtaggg tttgctggta aggctggcaa catcaatgcg actgtctcca tctgccatcg
     2881 gcagggccat caggagcccg gtaagcagct gtgagcctga cgatccgtca ataaaggccc
     2941 ggcccggcct tagtctccct gaaagcttca gggggagcag ccctccggag gtctccacct
     3001 ttacccccaa ctgtatgagc gcttccctga tcatggtgac cggacggcgc acaagtgagc
     3061 cttcaccggt gaaagtaaca ctttcagaga gcagcccggc cacaggggca aacatacgca
     3121 gagccagtcc tgattcgccg cagttcagcg ttacaggtcc ggcaggtctg atccctgcga
     3181 tgatatttat tctttcttcc tgctgcatta tggttgcacc cagagcctgc gccatgttta
     3241 tggctgccat ggaatcattg cagaatgagg cattccggat aaccgtcgtc ccgcctgaaa
     3301 gtagccctgc agcaatagcc ctctgagtca ggctcttgct tggcggagca tctgtcaggc
     3361 ctgccaggcc ggaaggattg acggtattgt tcatgaggtt gaaatatgtc ttcttaggca
     3421 aattccggcc actaatttaa ccttctttca tcactttcat ctggtgagtg atcgattcct
     3481 ggtgaatcgc ctccaggacg gcctgaatga actccggtgt cagtcccttc ctgaccccct
     3541 tgtccactgc gttgtcctta acctctctcc agcggctgct ctgaaggatt gtgatgccat
     3601 gttccttctt atagcggccg atggtctccg ctacctgcat cctgctctca aggatgtcaa
     3661 gcaggaggtt atcatacata tcaatctggc tgcgaagatc ttctatggtc tcaagaaact
     3721 caggatcatc cgtggttggt gaccgtaata ccaggctgcc gaggaccttc atcagctcca
     3781 cgggagtgat ctgctgtttt ctgtcgctga gggcgcctgc cggattaatg tgagactcta
     3841 tcatcagtcc gtcataatgc aggtccattg ctttctgtga aagatcacca atgaaatcac
     3901 ggtcacctcc catatgactc gggtcacaga ggagtccgat gccgggaatg cgctgacgca
     3961 gctcaacggc aatctgccag cggggctggt tgcggaatga gcatttctcc caggtggaga
     4021 agccacggtg cacagctgca atgcgcgtga tgccggcacg gctgatacgc tcaagtgccc
     4081 ctatccacag ttcaaggtca ggattgaccg gattcttgac aaacaccatt acatctgaat
     4141 ccttcagagc agaggcaatc tcatgcatag caaacggatt cacgctggta cgggcaccaa
     4201 tccagacggc atcaactcca tgtttgagag cttcataaac atgcctttcc gtagccactt
     4261 ccacagcaac cggaagacca tattcatcct ttacccgtct cagccaccgt agacccggtg
     4321 acccaacccc ctcaaactgc ccgggacggg tgcgtggttt ccagataccg gcacggaaga
     4381 gatccacccg gcccgaggcg gacagggcag cagcagtggc caatacctgc tcctcgcttt
     4441 cggcgctgca gggaccggcg attaccagag gccgtttctt gaagagcggc cattcctcca
     4501 ccggcacaac attcagcctg ttccggattc cgggggcaat cattgtcaaa tcatcagttt
     4561 tcatggtatt gtcagagttt catttaagga ttttcccgat actgtttgca tttttcataa
     4621 gctgcctcag cgcttcgccg ttgccgcttc cgatatgctg cctgaacagg agcatcttgt
     4681 ctatataggt gtcaataacc tcaagtattg gacccgcatt tccggtgaag atcggagccc
     4741 aggtctcccc gctgcttttc gcgagacgta ctgtgctctc aaaaccgcct gcggcgaggg
     4801 acaaaatgtt ggctgcatcc ttctccttgt caagtacgca gagtgcaagt gcaaacgagg
     4861 ttatatgtga tatatgggaa acatatgcca cgtggatgtc atgctccctg gaatccatgt
     4921 actgtatttt catgtttaat gattgcagca gggctgtgat cttctccatt gcatcttcgt
     4981 cgctccggtc cggatcacag attattgcca ccctgtcact gaacaggtgg tcaactgctg
     5041 ctgcaggacc tgagtgttcc gtcccggcca taggatgtgc cgccacaaac ctcccccgtg
     5101 cagggtgacg gtctgccact gcggcgatat ctgcctttgt tgatcccatg tctgtcacca
     5161 cctttcctgt cccggtaaca atatcaagta ttcccggcag aagccggcaa tttacatcca
     5221 ccggtgcagc aagaacaata atatcagact tcgccacggc ctcttccagc ggcaggaact
     5281 catcggccag accacgatac atggctattg tacagtgatg agggtttgcc tcaaccccaa
     5341 tgatcctgcc ggtgaatccg ttgagtctga ggcttcgcgc cagcgagcct ccgatgaggc
     5401 ccagtcccgt tatgcaaatg tttagtgaca tgtttcttca ggctgttttt agttggttgg
     5461 tattgactct ctccctgacc cttatgagcg ctcctgagag cagctcttca ctggcacaaa
     5521 gggaaatcct gatgtgcctc cggccgttgt tgccgaaaat tgatcccggg gtaacgaata
     5581 ggtgattatt gtgaagaagt tcctccgtaa gttcttctgc atcattgtag ccgtcgggta
     5641 tcttccccca aaggaacagc cctgactgcg aatcatcgaa agtgcacatg agtgcgttca
     5701 ttatctgagc cgccaggata cgcctccggg agtagaccga gttgagctct tcataccagc
     5761 tttcagggag ggcaagtgca gcagcggcgg caacctgtag aggctggaac attcctgagt
     5821 ccatgttgct tttaaccttc aacacattat ttatatatgt actgttgccg gcaagcatac
     5881 cgatcctcca gcctgccatg ttatgtgatt tgctgagcga attgagctca agcgctgtct
     5941 catcagcccc cgggactgag aggatactca ggtgatcatt gttaaggatg aagctgtacg
     6001 gattgtcgtt gcaaagcagg atccggttcc gccgggcaaa tctgacaagt tcctcaaaga
     6061 gcgtatatga ggcccgggcc ccggtaggca tatggggata gttgatccac atgatcttca
     6121 ccctgtctgt tccggaggtt tcaattgcac gtaaatcagg ccgccaccca ttttcttcac
     6181 tgaggtcata gctcctgacc acccctcctg caagttttgc tgcagatgca tatgccgggt
     6241 aaccgggatc aggtaccaga acctcgtcgc ccggatcaag aaaggccatg ctgatgtgca
     6301 t
//
LOCUS       DAOPXU010000136         6270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_15356_length_6270_cov_14.007884, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000136 DAOPXU010000000
VERSION     DAOPXU010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15356_length_6270_cov_14.007884"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PKK03_10960"
     CDS             complement(<1..404)
                     /locus_tag="PKK03_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84959.1"
                     /translation="MGIIVYIIVFGLVLLILLPFVIGHFRNVGKDKSMIVALKASASA
                     KGLSLTATDAWKGAYAIGIDAGRGTVVYLRKAGGSMQEEMTELSGVTDCRVDITARTE
                     KTPNGSMTVVEGISLVITFGDRALSGKRLEFY"
     gene            complement(544..1134)
                     /locus_tag="PKK03_10965"
     CDS             complement(544..1134)
                     /locus_tag="PKK03_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84960.1"
                     /translation="MRNSEGTWRRSAKQIWCLAQGCEGVKTPFEEVKTPCARAATESG
                     DSPKAEASELPGVSVRLMVPNLICYLCIMVRQRHFEGLLLGLTFLFLCYGIRGYYPRY
                     NEPLQEDAPFSRTAITEGNYPELLILNKGTGNPADALKQPPAPFDRTVAHGLSDLPVW
                     SGAGEIVKETAFRSYAGTVYIDVSSTDLIFPFHYFL"
     gene            1245..1601
                     /locus_tag="PKK03_10970"
     CDS             1245..1601
                     /locus_tag="PKK03_10970"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX84961.1"
                     /translation="MTDISFNKWSSSSDKGLTEQIGAFVRHHRIEQNKTQGELAKAAG
                     ISRSTLSLLERGETVTVTTLIQVLRVLDQLNIMSAFEVRENISPLALARLQKEKRQRA
                     RGRSAKKEESSEGSEW"
     gene            1605..2909
                     /locus_tag="PKK03_10975"
     CDS             1605..2909
                     /locus_tag="PKK03_10975"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HNX84962.1"
                     /translation="MNVAEVKIWGKPAGAVAWDEKTGFATFEYDPDFTRLGWDLAPLK
                     MPVADSGRVFAFPELRKDRNTESDTFRGLPGLLADALPDKYGNSLINVWLAQQGRPPE
                     SMNPVEMLCFIGTRGMGAMEFEPPVLKEYIRPFSIEIHSLVDTARRILSRREAFTINL
                     RQDEEKAVQEILKTGTSAGGARPKAVIAWNEKTGEVRSGQTKALRGFEHWLIKLDGVS
                     EVQMGVSHGYGRVEMAYYNMAKACDIEMMPSRLLEENGRAHFMTRRFDREGAETKHHV
                     QTFCALKHFDYNLVNSFSYEQLFQCMRELKLTYADAEQMFRRMAFNVVARNCDDHTKN
                     FSFLLKHGGRWELAPAYDLCHAYRPGSEWVSQHSLSINGKRKDITKADLLVIGDLIRL
                     RKASEIIDEINETVNRWKNYADEVHVSPKLRDEIGKTLLNLR"
     gene            3044..3901
                     /locus_tag="PKK03_10980"
     CDS             3044..3901
                     /locus_tag="PKK03_10980"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX84963.1"
                     /translation="MKQPELGRKITLLRKEKGLTQEELVDRCNISVRTLQRIEAGEVT
                     PRSYTVKTILAALDYDLSRISDKDSDRRSFAGWLKDIFLIDIDPDRSGTFIIKQLNIA
                     WLLGLVYFILGFLESAADYFRMEDDRMIFSIPVYVTIKVAVLITYFYFQRGFILVGHM
                     FRNYLLKIMSYVLIFGIFLIIAYDIASLFYESVEREYILGAQALTFGGILILYGISLI
                     KLQRSFGHVPVIAGIFEIAAGFFFLTIILSFIGYIMLIPAELLEIVILFKAVEIIKSK
                     QPVNGAMTS"
     gene            4061..5968
                     /locus_tag="PKK03_10985"
     CDS             4061..5968
                     /locus_tag="PKK03_10985"
                     /inference="COORDINATES: protein motif:HMM:NF024295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HNX84964.1"
                     /translation="MRKICLLASLLLLAIRPGKAQNITIQVNANEGRMTVSPYIYGRN
                     NNLSDDPAKPTTEANINLYKEAGLRFARENGGNNATKYNWRRKLGSHPDWYNNVYGHN
                     WDFASQDIQTRMPGLQVMWAFQLIGKAAGNRNNNFNDWSYNGSNWWSGVGQNLAGGGQ
                     VNPAGGSQALVNGNPDLYLVNWTADSTTDVLNHWFGTGGLSLDKNQFTYWSMDNEPEI
                     WNGTHDDVMPSLLSASAFMDSYFAVAKKARERFPEIRLTGPVPANEWQWYKWGNEALT
                     LGGKYYCWLEYFIKRVADEQKATGIRLLDVLDVHWYPSESSNADLVQLHRVFYDETYV
                     YPGANGVKTINGGWDGAQTKEYIFKRINGWLNTHFGENHGITIALTENDISSSNPNIV
                     SVVYASMLGTFADNGVEIFTPWSWKTGMWETLHLFSRYAKSTRVRTTSTGESTVSGYS
                     TVNSSNDSLTVILVNRDLSASRTVTVNLAGFTVPNGSYNILELSALPSTETFVSHTTN
                     AIKSKTVAVSGNSFTLTLPSLSTTAVILAGTGTGIEENQPESGDSNTLRAYPNPTSGK
                     FTIHLDEQVPGDLTLKFTNLTGNMVLTLKAGNDSEIDLSQTTLDGGMYVVTAENEGRC
                     WRTKLIYERGK"
BASE COUNT         1687 a         1570 c         1556 g         1457 t
ORIGIN      
        1 ttgtaaaact ccagcctctt cccgctcaaa gccctgtcac cgaacgtaat tacgaggctg
       61 atgccttcga ccactgtcat gctcccgttc ggagtctttt cagtcctggc cgtaatatca
      121 accctgcagt ctgtgacacc tgacagctcc gtcatctctt cctgcattga ccctccggct
      181 ttcctgagat aaaccaccgt gcccctgccc gcgtcaatgc caatggcata ggcccccttc
      241 caggcatctg ttgcggtcag tgaaagtccc tttgcagacg ctgaagcctt caacgcgacg
      301 atcatcgact tatccttacc aacattcctg aaatgtccga taacaaacgg caacagtatg
      361 agcagcacca gtccaaaaac tataatataa acaataattc ccattttcgt atgtaattat
      421 aaatgaaaca tatagctgcc tgaacaacac gctatggaac aattgccaca tgtacgctgt
      481 tcggggcagc aattaattaa acgacagatt gttgccaggc tgatgaaacc agccggcact
      541 gacttacagg aaataatgaa acgggaaaat caggtcagtt gaagatacat ctatataaac
      601 cgttccggca tatgaccgga aggctgtttc ttttactatc tcgcccgctc ctgaccagac
      661 cggcaggtct gacagaccat gcgccactgt tctgtcaaag ggagcaggtg gttgtttgag
      721 ggcatcggca ggattgccgg tccccttatt caggatcagc agttccggat aattgccttc
      781 ggtaattgcc gtgcgggaaa acggagcatc ttcctgcagc ggttcgttat accgcgggta
      841 ataacccctg attccatagc aaaggaaaag gaacgtaagt cccagaagta atccttcaaa
      901 atgcctttgc ctgaccataa tgcaaagata gcaaattaag tttgggacca tgagcctgac
      961 gcttacacct ggcagttctg aagcttctgc cttcggcgaa tcgcctgact ccgttgccgc
     1021 ccttgcgcaa ggtgtcttca cctcttcgaa tggtgtcttc accccttcgc acccctgcgc
     1081 aaggcaccat atctgtttcg cgctccggcg ccatgtgcct tcactgtttc gcaccccggc
     1141 gcaaggaaaa aacagatcaa ttcttcctct aaaccttgga tattcaaaat attatagtta
     1201 tatttgtatg atttatcatt cactaatagc atttattaaa cattatgact gatatttcat
     1261 tcaataaatg gtcttccagc agtgataaag gcctgactga acagatcggc gcctttgtga
     1321 ggcatcaccg tatagagcag aacaaaaccc agggtgaact ggcaaaagca gccggtatca
     1381 gtcgctccac cctgagcctg cttgagcggg gtgagacagt cacagtcaca acactgatcc
     1441 aggtgctgag ggtattggat cagctgaata tcatgagcgc ttttgaagtc agggaaaaca
     1501 tcagtcccct cgctctggcc aggttgcaga aagaaaaacg acaacgggca cgaggcagat
     1561 ccgcaaaaaa ggaagaatct tctgaaggct cagaatggta aggcatgaac gtagcagaag
     1621 taaagatatg ggggaagccg gcaggcgccg tagcctggga tgaaaaaacg ggctttgcca
     1681 cctttgaata tgaccctgat ttcactcgcc tgggctggga cctggctccc ctgaaaatgc
     1741 ctgttgctga ctccggacgg gtttttgctt ttccggaact caggaaagac agaaacacgg
     1801 aatccgatac cttcagaggg ttgcccggtc tgcttgcaga tgccctgccc gacaaatacg
     1861 gcaacagtct gataaatgtc tggctcgcac agcagggacg cccgccggag agcatgaatc
     1921 cggtggaaat gctctgcttc atcggcaccc gtggcatggg agccatggag tttgaaccgc
     1981 cggtgctgaa agagtacatc aggccatttt caatagaaat ccacagcctg gttgataccg
     2041 cccgaagaat tctcagccgg cgtgaggcct ttacgattaa tctcagacag gatgaagaga
     2101 aggctgtaca ggagattctg aaaaccggca catcggccgg tggcgcccgt cccaaggccg
     2161 tgatagcctg gaatgagaag accggggagg tacggtcagg gcagaccaaa gcactcaggg
     2221 gatttgaaca ctggctgata aagctcgatg gcgtcagtga ggttcagatg ggagtaagtc
     2281 acggttacgg gagggttgag atggcatact acaatatggc gaaggcctgc gacattgaaa
     2341 tgatgccatc gcgactcctg gaagagaatg gcagggcgca cttcatgaca cggcggttcg
     2401 accgtgaggg cgcggaaaca aagcatcatg tgcagacatt ctgtgccctg aagcatttcg
     2461 attacaacct ggtcaacagc ttcagttacg agcagctttt ccagtgcatg cgggagctta
     2521 aactgaccta tgccgatgct gaacagatgt tcagaaggat ggctttcaac gtcgttgcaa
     2581 ggaactgtga tgatcacaca aagaactttt cattcctgct gaagcatggg ggcagatggg
     2641 aactggcgcc ggcctatgac ctgtgccacg cttaccggcc gggcagcgag tgggtcagcc
     2701 agcattcact cagcatcaac ggcaagcgta aggatattac caaggccgat ttgctggtaa
     2761 ttggcgactt gatcaggctc aggaaagctt ctgaaatcat tgatgagata aacgagacag
     2821 tcaaccggtg gaagaattat gctgacgagg ttcatgtgtc accgaagctg cgcgacgaaa
     2881 tagggaaaac ccttctgaac ctccggtgat gaattgcgcc tgtaagtctc tctttttcag
     2941 tcttttccca aatgtcgggt aaatgacggg caaagtaccg tcaaaatatt ttttgcattg
     3001 ggttttcaat cgtaatattg gtgaagattc agaaatcaag acaatgaaac aaccggaatt
     3061 aggcagaaag atcaccctcc tcagaaaaga aaaaggtctc acccaggaag aacttgtaga
     3121 caggtgtaat ataagtgtac gaaccctcca gaggatagaa gccggcgagg tcaccccaag
     3181 aagctacaca gtaaaaacaa tcctcgcggc tctcgattac gacctgagca ggatatcaga
     3241 taaagactca gacagaagga gtttcgccgg atggctgaag gatatctttc tgattgatat
     3301 tgaccccgac cggtcaggca cttttattat taaacagctt aacattgcgt ggctgctcgg
     3361 cctggtctat ttcattctcg ggttcctcga atctgcggca gactatttcc ggatggaaga
     3421 tgaccggatg atcttcagca tcccggtata tgtaactatc aaggtggctg tcctgatcac
     3481 ttacttttac ttccagcgag gcttcatcct tgtgggccac atgttcagga actacctact
     3541 gaagatcatg tcatatgtcc tgatctttgg catcttcctt ataatagcat atgatattgc
     3601 ttcactgttc tatgaatccg ttgagcggga atacatactg ggagcccagg cactgacttt
     3661 cggagggata ctgatccttt acgggatctc gctgatcaag cttcagagat cattcgggca
     3721 tgttccggtt atcgcaggta tctttgagat tgccgcaggc ttcttcttcc tgacaatcat
     3781 actgtcattc atcggatata taatgcttat cccggccgaa ctgctggaga ttgtgatcct
     3841 ctttaaggca gtcgaaatca ttaaatcgaa gcagccggtg aacggggcga tgacatcgta
     3901 aggcgcgtgc tggcactgaa ggccgctact gatcctgata atgcaatact gcagtccccc
     3961 tcggcaccgg acaggtcccc tgaattggtt gacaaaataa gagctacctt tgtgaaccca
     4021 caggtcagta tcggtaatac tctgtcctgg tgcagttatt atgagaaaga tctgtctgct
     4081 tgcttcgctc ctgctgctgg ccatccggcc ggggaaagca cagaacataa ccatacaggt
     4141 aaatgcgaac gaaggacgca tgactgtttc gccatacatc tacggccgga acaataacct
     4201 ctccgatgac ccagcaaaac ccaccaccga agcaaatatc aacctgtata aagaggccgg
     4261 acttcgcttt gcgcgcgaaa acggaggaaa caacgccacc aaatacaact ggcggaggaa
     4321 gctgggaagt caccccgact ggtacaacaa tgtctatggc cataactggg atttcgcatc
     4381 gcaggatata cagacacgga tgccgggact gcaggtcatg tgggctttcc agctgatagg
     4441 gaaagctgcg ggcaacagga acaacaactt caacgactgg tcgtacaacg gatcgaactg
     4501 gtggagcggc gttggccaga atctggccgg gggcggccag gtgaatcctg ccggcggctc
     4561 ccaggcactt gtgaacggaa accctgatct ctacctggtc aactggaccg ccgattcaac
     4621 cacggatgtt cttaatcact ggttcggaac gggaggtctg agcctggata agaatcagtt
     4681 cacttactgg agcatggata atgagccgga gatatggaac ggtacccatg acgatgtgat
     4741 gccttcactc ctgtctgcct cggctttcat ggattcatat ttcgccgtgg cgaagaaagc
     4801 ccgcgagagg tttcctgaga tcaggcttac aggcccggtc cctgccaacg aatggcagtg
     4861 gtacaaatgg ggtaatgaag cgctgaccct cggcggaaag tattattgct ggctcgaata
     4921 ctttataaaa cgtgttgccg acgaacagaa agcaacagga atacgcctcc ttgatgtgct
     4981 cgacgtccac tggtacccgt cggaatcatc gaatgccgac cttgtacagc ttcaccgtgt
     5041 attctatgac gagacttacg tgtaccccgg cgccaacggt gtgaaaacga tcaacggcgg
     5101 ctgggacgga gcacagacca aagagtacat tttcaagagg ataaacggct ggctgaacac
     5161 tcatttcggc gagaatcacg gcataactat tgctctgact gaaaacgata taagctcatc
     5221 aaacccgaat attgtttcag tggtttacgc ctccatgctt ggcacatttg ccgataacgg
     5281 tgtggaaata tttactccgt ggtcatggaa gaccgggatg tgggagacgc tgcacctctt
     5341 cagccgctat gcgaaaagta ccagggtcag aaccacctca acaggcgagt caaccgtctc
     5401 aggctacagc accgtcaaca gcagcaatga ttcactgacg gtcatccttg tcaaccgtga
     5461 tctgagtgca tcgcggacgg taacggtaaa ccttgccggc tttactgtcc ccaatggcag
     5521 ctacaatatc ctcgagctgt ccgctctgcc ctcgaccgag acattcgttt cgcacaccac
     5581 aaacgccata aaaagcaaga cagtagcagt ttccggcaac tcgttcacac tcaccctgcc
     5641 gtccctgtca accactgcag tgatacttgc cggaaccgga acaggcattg aagagaatca
     5701 gccggaatcg ggtgattcga acacgttaag ggcctaccct aacccgacat cgggaaaatt
     5761 cacgattcac ctcgatgaac aggtgccggg ggacctgacc ctgaagttca ccaacctgac
     5821 agggaacatg gtgctgaccc tgaaagctgg aaacgactcg gaaatcgacc tgtcacaaac
     5881 gactcttgac ggcggcatgt acgtcgtaac agctgaaaat gaaggccgct gctggcgcac
     5941 caagctgatt tatgaacggg gcaaataatt aagcttctcc atcatcctgt gtaagaacca
     6001 tttagaaaaa ttcttaaaga tttatcaaaa ataatgtctt attttcttga catcgtgtat
     6061 attttattta tctttgtgtc gttatacttt tactcatagt atataataac agacacgtgg
     6121 aggtattaaa atgggacacc atgaaaaaca atcaatcgta tcaatcatcg gctcagtgct
     6181 ggtgatcgga ttctactcgc tctatgttta caggaattac atccaggcag atatggagct
     6241 gctgaacgat tttcagttct gggggaaatc
//
LOCUS       DAOPXU010000137         6220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_15536_length_6220_cov_13.975020, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000137 DAOPXU010000000
VERSION     DAOPXU010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15536_length_6220_cov_13.975020"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /gene="gatD"
                     /locus_tag="PKK03_10990"
     CDS             complement(<1..1361)
                     /gene="gatD"
                     /locus_tag="PKK03_10990"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003217.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HNX84965.1"
                     /translation="MAEDFFQGYKGEALAVLKKFNVRVWGQADIETTRGLFKGTILPR
                     SENNDDLHIVIKIETGYNIGIDIATIIRMTETGYNKANYKIPEKEFPYSDDKPNVKLF
                     GTGGTIASRLDYRTGAVIPAFSPGELYGAVPELADVCNIRTEKLFAVFSENMGPDQYK
                     ILAKAIGDEISNGIDGIMIGHGTDTLHHTAAALSFMVQNSPVPIVLVGSQRSSDRPSS
                     DAALNLIHASTAAGHGDIAEVMVCMFGPTSDEYGFLHRGTRVRKMHSSYRSTFRTIGD
                     IPVATVKRGEVKPIQKIYNPRRTDRNVKIMPYFNENVTMIYFYPHMKADMIHSLIDNG
                     YKGIIIVGTGLGHVNRELYPALERARRENVAVYMTLQTIWGYVHMFVYETGRDMMAKG
                     VIPLGNMLPEVAWVKLGWALGQTDDLEEVKRIMLTPVNGEITEREPYNGYLVYQGGVP
                     EVE"
     gene            complement(1367..3349)
                     /gene="gatE"
                     /locus_tag="PKK03_10995"
     CDS             complement(1367..3349)
                     /gene="gatE"
                     /locus_tag="PKK03_10995"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HNX84966.1"
                     /translation="MSIIDPRKNYEETREEIGYVTRKEATLNDYKRIGFKSGLEVHQQ
                     LRTKSKLFCRCPAGIYHDDNDFDAEVIRHMRPTLSEMGVYDGTALMEFRTRKEITYRI
                     NNETACTYEVDDTPPFPVDREALEIAISIALAARMNIVGEVHITRKQYLDGSIPTGFQ
                     RSAIISVEGELQLKNKRVRLIQLSLEEDSCREVSDIGHKRIYKTDRLGMPLIETVTYP
                     DCLTPDELAEAAEYIRFLNRSTGKVRTGMGAAREDVNVSCEGGSRAEIKGVAHIRWIP
                     ELSHNEAFRQYALLNIRSKLHKNIEDPAAWSAKTKEITEREQEFHFAPVRSALDRGDR
                     VIALNLPGCRGILSHFTQPGRIFADEISDRLKVIACIEKPNMTHSEEIAGGNFNNLFP
                     ELAIWLSADAGDAQIILWGPGEDIPTAIETVEERIRLAFAGVPNETRKAFEDGTTMFE
                     RVLPGRDRMYPDTDSQPIAVTTEMLEKLGRNMPVDVAKRFLQMKEWNLPEDTWHYLLS
                     KNLVVLVERISNDLGFDPREVGIMIGHRFRSLEGRGRVAAGFSYERLYDLFAFLKNEK
                     LLAPVARILLPAAVAEASPDFKSILAGTGLHRATMNEIRDEAKNLAARFPEVCYNGNM
                     AGMEEWMMGQLHMKALGTVRLSEVRNAVSELLEVRD"
     gene            complement(3579..4823)
                     /locus_tag="PKK03_11000"
     CDS             complement(3579..4823)
                     /locus_tag="PKK03_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX84967.1"
                     /translation="MSIGTFIASRLIKDRNRGTAFSRPINVIAIIGIALGLAVMILAV
                     SILTGFKQQIRDKVAGFGAHIQVVNFDANLSYETVPVSSNQPFVSKIREHRGIRNVQV
                     FATKAGIIKTDENIQGVVLKGIGSDYDWSFFRSNMVEGETLVLSDTARSNDVVISKKV
                     ADMLGLKLGDSFSMFFVQDPPRMRKFTILGIYETSLEEFDKTYLFCDINHIRSLNGWS
                     DDQVSGFEIYINDFEELDYMTDVVRDAIGYRVAEEEEQLKVTNIRGRYPQIFDWLGFQ
                     DTNVIIILVLMILVAGFNMISGLLILILEKTNMIGVLKALGAGNKVIRSVFLYQAAWL
                     TARGLFWGNVIGIGLALLQLKTEMISLDPSSYYLRSVPINLDPVHVILLNIGTMIAII
                     LMLLIPSQLIARITPVKAIRYD"
     gene            4901..6076
                     /locus_tag="PKK03_11005"
     CDS             4901..6076
                     /locus_tag="PKK03_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNX84968.1"
                     /translation="MKKFVIPLIIVTVVIAACAAGNKNTTLMTGAERTGEYLPLLKGK
                     RVAVVANQTSMVGKTHLIDTLLSEGVNVRLIFAPEHGFRDLADDGAVITSGTDPVTGI
                     EVISLYSSKKKPAPEDLADIDVVVFDIQDVGTRFYTYLTTMCYVMEACAENGKQFIVL
                     DRPNPNGYYVDGPILDTAGYRSFVGIHPIPVVHGMTFGEYAGMTNGEGWLAGGIRCDL
                     KVIGCTAYTHSTMYQLPVIPSPNLPNMNSIYLYPSLCFSEGTVLSCGRGTGFAFQVLG
                     APGMPDTGFSFTPQPSFGSSNPKHNGLTCYGIDLRNATGDGLVPKPEINLDWIIDMYR
                     AYPDKENFFTGYFNTLAGSSTLREQIISGMSAAEIRASWKEGLEAFKTIRSKYLLYD"
BASE COUNT         1422 a         1737 c         1545 g         1516 t
ORIGIN      
        1 tcctccacct ccggaacgcc gccctggtac accaggtaac cgttatacgg ctcccgctcg
       61 gtgatctcgc cattgacggg agtcagcatg atccgcttca cctcctccag gtcatcggtc
      121 tgccccagcg cccaacccag tttaacccac gccacctctg gcagcatgtt gccaagcggt
      181 atcacaccct tcgccatcat gtcacgaccg gtctcatata caaacatatg aacataaccc
      241 cagatggtct gcagcgtcat gtataccgcc acattctcgc gccgggctct ctcaagggcc
      301 gggtagagct ccctgttcac gtgcccgagg cctgttccca ctatgataat ccccttgtaa
      361 ccgttgtcaa tgagtgaatg gatcatatcc gccttcatat gcggatagaa atagatcatt
      421 gtcacattct cgttgaaata tggcatgatc ttcacattgc ggtcagtgcg ccgcggattg
      481 tatatcttct ggatcggctt cacctctccc ctcttcaccg ttgccacggg tatgtcaccg
      541 attgtccgga aagtggacct gtacgaactg tgcatcttac gcaccctggt gccgcggtgc
      601 aggaaaccgt actcatcact ggtgggaccg aacatgcaga ccatcacctc ggcgatgtca
      661 ccatgcccgg cggccgttga ggcatgtatc aggttgaggg cagcgtctga cgacggccgg
      721 tcagatgacc tctgcgatcc taccagcact atcggcacgg gcgagttctg taccatgaac
      781 gagagcgctg cagccgtgtg atgcagggtg tcagtgccat gaccgatcat gatgccgtca
      841 attccgtttg aaatctcgtc acctatggcc tttgcaagta tcttgtactg gtcggggccc
      901 atgttctcgc tgaatacggc gaagagcttc tccgtgcgta tgttgcatac gtcagccagc
      961 tcgggcacgg caccgtagag ctcacccggc gaaaatgccg gaatgactgc acccgtgcgg
     1021 taatcaagcc tcgaggcaat ggttccccct gtgccaaaca acttcacatt gggcttgtca
     1081 tcagaatagg gaaattcctt ttcgggaatc ttgtaattgg ccttgttgta accggtctcc
     1141 gtcatcctga ttatggtagc aatgtcgatc ccgatattat aaccggtctc gatcttgatc
     1201 acgatgtgga gatcgtcatt attctctgaa cgcggcagga tggtcccctt gaacagtccc
     1261 ctggtagtct cgatgtcagc ctgaccccag acgcggacat taaacttctt cagcaccgca
     1321 agcgcctcgc ctttgtatcc ctggaaaaaa tcttcagcca taacagtcaa tctctaacct
     1381 caagtaattc tgacaccgca tttctcactt ccgacaacct cacagtcccg agggctttca
     1441 tatgcaattg tcccatcatc cactcttcca tccccgccat attaccgttg tagcagactt
     1501 ccgggaacct tgcggcaagg ttttttgcct catccctgat ttcattcatc gtggcacggt
     1561 gaagcccggt tccggccagt atgctcttaa aatcaggtga tgcttctgca actgcggcag
     1621 gaagcaatat tcgcgccacc ggcgcgagaa gcttctcatt cttcaggaag gcaaagaggt
     1681 catagagtct ttcatagctg aaccctgctg ccaccctgcc ccgcccctcg aggctgcgga
     1741 aacggtggcc tatcattatt ccgacctccc tgggatcaaa gccaagatca ttgctgatcc
     1801 gctctaccag taccaccagg ttcttgctca gcaggtaatg ccaggtgtct tcgggaaggt
     1861 tccattcctt catctggagg aacctttttg caacgtcaac cggcatgttt ctgccaagtt
     1921 tttcaagcat ctccgtcgtg acagctatgg gctgtgaatc ggtgtcaggg tacatgcggt
     1981 cacgcccggg aagaaccctc tcaaacatgg ttgtgccatc ctcaaacgcc ttgcgggttt
     2041 cattgggcac gcccgcgaaa gcgaggcgga tccgttcctc gactgtttca atggcagtgg
     2101 ggatgtcttc accagggccc cagagtatta tctgtgcatc accggcatca gccgaaagcc
     2161 atatggccag ttccgggaag agattgttga agttgccccc ggcgatctcc tccgaatggg
     2221 tcatgttggg cttctcgatg caggctatca ctttaagcct gtcacttatc tcatcggcaa
     2281 agatccttcc gggctgggta aaatgcgaaa gtatacctct gcaccccggg agattcaggg
     2341 caatgacccg atctcctcgg tcgagagccg acctgaccgg tgcgaaatga aactcctgtt
     2401 ccctttctgt gatctccttt gttttagccg accatgcagc cgggtcttct atgttcttgt
     2461 gcagtttcga gcggatgtta agcagcgcgt actgcctgaa tgcctcgttg tgtgacagct
     2521 ccgggatcca cctgatatgt gccaccccct tgatctcagc ccggcttccg ccctcgcagc
     2581 tgacgttcac gtcttcgcgc gcagcgccca tgcccgtgcg taccttgccg gtactgcggt
     2641 taaggaacct gatatattcg gccgcctcgg ccagctcgtc aggggtgagg caatcaggat
     2701 aggtcacagt ctctatcagc ggcatgccaa ggcggtcagt cttgtatatc cgtttgtgcc
     2761 ctatgtctga tacttcgcgg catgagtcct cctccaggct gagctggatg agccttaccc
     2821 tcttgttttt cagctgaagc tctccctcaa cgctgatgat ggcagagcgc tggaacccgg
     2881 tgggtatgct tccgtcaaga tactgcttac gggtgatatg cacctcaccc acgatgttca
     2941 tcctggcagc cagtgcaatg cttatggcaa tctccagcgc ctcccggtca acggggaacg
     3001 ggggggtgtc atcaacctcg taggtgcatg ccgtttcatt gttaatacgg taagtgatct
     3061 ccttcctggt cctgaattcc atgagtgcag tgccgtcata aactcccatc tcactgagag
     3121 tcggtctcat gtgacggatc acctccgcgt caaaatcatt gtcatcatgg taaataccgg
     3181 ccggacaacg gcaaaagagc ttgctcttcg tcctcagctg ctggtgcacc tcgaggcccg
     3241 atttgaagcc gatccgctta taatcattaa gcgtcgcctc cttacgggtg acatacccga
     3301 tctcctccct ggtttcttcg tagtttttcc gcggatcaat tattgacata ccttaagaca
     3361 ttgcctctgc aatttacgca gaagcgcgga atattggaag tgcccattta aagaaagttt
     3421 gcgtaacttt ctgctataac atgtttcaat gccggacaat taaaattatg tcgaaatcaa
     3481 agggcgggga tttaaaaatt aaaccctgcg gaaaaaaatt gacatttgca ttaaaacggg
     3541 atccacttaa taatattaag cgcgtagcga cctgattatc agtcgtagcg aattgccttg
     3601 acgggtgtaa tgcgggcaat aagctgagaa ggtataagaa gcatcaggat gatggcaatc
     3661 atcgtcccga tgttaagcag gatgacatga acgggatcaa gattgatcgg aacgctcctg
     3721 agataatatg aggatgggtc cagcgaaatc atctctgttt tgagctgaag cagtgccagc
     3781 ccgatgccta ttacgttgcc ccagaaaagg cccctggccg tcagccaggc agcctggtaa
     3841 agaaagacgc tgcggataac cttgttgccc gcgccaagag ccttcagcac ccctatcatg
     3901 ttggtcttct caagtatcag aatgagcaga ccggaaatca tgttgaaccc ggctacaagg
     3961 atcataagca ccaggatgat tatcacgttg gtgtcctgga atcccagcca gtcgaatatc
     4021 tgcgggtaac gccccctgat gttggttacc ttgagctgct cctcttcctc ggcgacccgg
     4081 tagcctattg catcacggac aacatccgtc atgtaatcaa gctcttcgaa gtcatttatg
     4141 taaatctcaa aaccgcttac ctggtcatcg ctccagccgt tcaggctcct gatgtggttg
     4201 atgtcgcaga agaggtaggt cttgtcaaac tcctcgaggc tggtctcata gatgccgaga
     4261 atggtgaact tgcgcatccg gggcggatcc tgaacgaaaa acatcgaaaa cgagtccccc
     4321 agcttgagac ccagcatgtc agccaccttc ttcgagataa ccacatcatt gcttcgtgcc
     4381 gtgtcactga ggaccaacgt ctctccctcc accatgttcg atctgaagaa actccagtca
     4441 taatcactgc caatcccctt tagaacaact ccctgaatgt tttcgtcagt cttgatgatt
     4501 cccgccttcg tggcgaagac ctgcacgttc ctgattcccc ggtgctccct gattttggag
     4561 acgaagggct ggtttgagct gacaggcacg gtctcatacg aaagattggc gtcgaaatta
     4621 accacctgga tatgagcccc gaagcccgcc accttgtcac ggatctgctg cttgaagccg
     4681 gtgaggattg acaccgcgag gatcatcacc gccagtccca gggcaatgcc tattatggct
     4741 attacattta ttggacgcga aaaggcagtg ccacggttgc ggtcctttat aaggcgactg
     4801 gctatgaagg ttccgattga cattgcgaca aaattaagaa ttaagagcga agaatccgtt
     4861 tgttgcctct tttcttatct ttacgctaaa cctgactgaa atgaaaaaat ttgtcatccc
     4921 gctgatcatt gtaactgtgg tcattgcggc ctgtgcggcc ggaaataaaa acacaaccct
     4981 gatgacaggg gccgagagaa ccggtgagta cctgccgctg ctcaagggaa aaagggtggc
     5041 ggttgtggca aaccagacct ctatggttgg caaaacccat ctgattgata ctctgctatc
     5101 tgaaggcgtc aacgtccggc tgatctttgc tccggaacat ggcttcaggg acctggccga
     5161 cgatggtgcg gtgatcacct cgggaaccga ccctgtgacg ggcatcgaag tcataagcct
     5221 ctattcctcg aaaaagaagc cggcaccgga agatctcgct gacatagatg ttgttgtatt
     5281 tgacatccag gatgtgggga ccaggtttta cacatacctg accaccatgt gttacgttat
     5341 ggaggcatgc gccgaaaacg ggaagcagtt catagtgctt gacaggccga acccgaatgg
     5401 ttattatgtc gacggaccga tacttgacac cgccggttac cgctcctttg taggaattca
     5461 tcccataccc gtggtgcacg gaatgacctt cggcgagtat gccggcatga cgaacggcga
     5521 gggatggctg gcaggaggca tcaggtgcga tctgaaggtg atcggctgca ccgcctacac
     5581 tcactctacg atgtaccagc tgccggtgat accgtcacct aaccttccaa acatgaactc
     5641 catatacctg tacccgtcgc tctgcttctc ggagggaaca gttctgtcat gcggaagggg
     5701 taccggtttt gctttccagg tgcttggagc tccggggatg ccggatacag gattcagctt
     5761 caccccgcag ccctcattcg gctcatccaa ccccaaacat aacggattga cctgttacgg
     5821 cattgacctt cgcaatgcga cgggtgacgg actggtcccg aaacctgaga taaacctcga
     5881 ctggatcatt gacatgtaca gggcgtaccc tgacaaggag aacttcttca ccggttattt
     5941 taacaccctg gccgggagca gcacgctccg cgaacagatt atcagtggca tgagtgctgc
     6001 ggaaataagg gcttcatgga aggaggggct ggaggcgttc aaaaccatca ggtcaaaata
     6061 cctcctgtac gactaataat gttttacccc ggtaattcac gactcgcaac ccgcaaattt
     6121 gagcatgcga cgcaggagca gtatcaatcc cgcatccgcc agccggcata tcgggaccca
     6181 actcgcaatt cacattttgt tgttgtcgca aatcgcaatt
//
LOCUS       DAOPXU010000138         6105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_15971_length_6105_cov_8.373554, whole genome shotgun sequence.
ACCESSION   DAOPXU010000138 DAOPXU010000000
VERSION     DAOPXU010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15971_length_6105_cov_8.373554"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1123
                     /locus_tag="PKK03_11010"
     CDS             158..1123
                     /locus_tag="PKK03_11010"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNX84969.1"
                     /translation="MTKRCLIIYNEPAPDALPDELDVLDQVNFINETLQSLGYQVECR
                     GITDNFFSEIGEIAEEEFDFVFNLVESVGKKAEILYFIPGLLNMNHIPYTGCPVEATF
                     ITASKVLARKIMKANGIPVAGGYRVSEASQLVPGKKYILKPVWEDGSLGITEESVFTF
                     NGTPPEILRGKNDRHWFIEEFIEGREFNVSVKAGPDGPEMLPPAEMLFEDYPDDIPRI
                     VSYKAKWVADTFQYENSRRNFPGNLSEKLMNNLRDAVNGCWHTFGLKGYARVDMRVDA
                     DENVYVLEVNANPCISPDSGFISAAVHAGYTHQEIIRHIINDLNT"
     gene            1131..1610
                     /locus_tag="PKK03_11015"
     CDS             1131..1610
                     /locus_tag="PKK03_11015"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX84970.1"
                     /translation="MPEISFKITPADRPAFEKILASSGFFYDFEIEVALSLADETFTH
                     GEDKSGYYWVKLVEDGEVIGFANFGPNPSSVHSWDIYWLAIQEEYRKKRYGSLILTKT
                     EDRIRSLGGKIAWIETSGRPLYEPTRHFYIRNGYELEATLREFYGPGDPKLVYRKEL"
     gene            1607..1975
                     /locus_tag="PKK03_11020"
     CDS             1607..1975
                     /locus_tag="PKK03_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNX84971.1"
                     /translation="MKGLWSILLLIVSNSFMTIAWYGHLKYQNVSKNALLGLLGIVLA
                     SWGIALFEYFFQVPANRIGYRGTGGPFSLVELKLVQEVISISVFVVFTLVFFRTETFK
                     LNHLFAFMCILGAIYFTFKG"
     gene            complement(1965..2981)
                     /locus_tag="PKK03_11025"
     CDS             complement(1965..2981)
                     /locus_tag="PKK03_11025"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HNX84972.1"
                     /translation="MIDLRSDTVTRPSAGMLERMMKASVGDMIFGEDPEVNELERMAA
                     EMFGMEGALFCPSGTMTNQIAIKAHTRPGDEVICSRMAHIYLFEGGGVAFNSGASVAL
                     IDTVNGTFTAEDVKQHINNPDDPHRAFTRLVEVENTVNRGAGACWDFKDLKRIRELCD
                     SRHLGLHLDGARLFNAMTADGRKPEEYGALFDSISICLSKGLGAPVGSVLIGNREIIR
                     QGVRIRKLFGGAMRQAGYIAAAGIYALKNNILRLADDHRNAAFLAEALNKCSLTEKVN
                     YGGTNIVIFTLKPGNTASEFLEKIKARGILALKTGERSIRMVTHLDVSDGQIREACDI
                     LASL"
     gene            3097..3759
                     /gene="ung"
                     /locus_tag="PKK03_11030"
     CDS             3097..3759
                     /gene="ung"
                     /locus_tag="PKK03_11030"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNX84973.1"
                     /translation="MEVKIEAGWKEKLSGEFEKEYFRTLTEFVREEYRTRQVFPPGNK
                     IFNAFDLCPLDRVRVVIIGQDPYHNYGQAHGLCFSVNDGTEFPPSLVNIFKELNRDLG
                     VPVPKSGNLERWASQGVLLLNAILTVRAHQALSHQNRGWERFTDAAISALNRERENLV
                     FLLWGAYAQNKGASIDESKHLVLKTVHPSPLSASRGFFGCSHFSRCNQWLVARGMEPI
                     QW"
     gene            complement(3760..4929)
                     /locus_tag="PKK03_11035"
     CDS             complement(3760..4929)
                     /locus_tag="PKK03_11035"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNX84974.1"
                     /translation="MKKREINSVRTPVYRDSGFELPTAAATSGAFAAETGHEREPDMY
                     IYSRYRNPTVVAAEEAVMEVEGCGWALLAQSGMAAIDIALSIFQEAGKQSNWLFFSEI
                     YGGTLSYIGSVLQKRRGMDVSFFRPVDGRYDYRQLENALSDIRPDILYFETISNPLLI
                     VAEGRRVIELGKKYGAKVIVDNTFATPLLWKPLDDGADMVIHSATKYLSGHGNLTAGV
                     ICGNDTNLMQSAIEYRKYVGHMLSPDDAYRLQTQMASFELRYRQQCDNAAAVASMLKS
                     SDLVAGVLYPGLESHDTHEAAKELTGGKGYGAMITFDLAGASPEAKRKNRDRFIAAVY
                     PEIRLIPTLGDNHTILMPVEAVWGTKYPEPGMIRLSVGYEKTDRLAEIIGRGLKG"
     gene            complement(4883..>6105)
                     /locus_tag="PKK03_11040"
     CDS             complement(4883..>6105)
                     /locus_tag="PKK03_11040"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX84975.1"
                     /translation="VINLSMACNHCAEPACASGCPALAYTISPDGVVIHHTDRCMGCG
                     YCTWRCPYGAPKINPALGYIEKCHFCADRTTQGVEPACVTACPTGALKMTEREEFDGA
                     LPAWFPDTGIKPSVFIKNAGTNHKPFIIPAGNESEEDVEEILTTQGTSSGSSGRLKEE
                     WSLALFSVLVITAYVILIVSALRGTITGGFMPFILMVCAMAVSVVHLGVPGKSWRAVL
                     NLLSSPLSREIAMVLMLAFVALLEWMKPDILPPLVTGLLALLTLISIDNVYFAADRSF
                     SLKLHSGQAFFTGLYAATWFIEPTIIFIVLSMLAAISVVMRYRSSEADSLTRALYYTR
                     AMALPMVFMLIYPDSPVTDIAALVIFITGLMADRLLFYCDFRPPNIKDTITEHFAKEY
                     EKERDKQRQDAGIS"
BASE COUNT         1474 a         1605 c         1539 g         1487 t
ORIGIN      
        1 gccacggaac gatgtgcttc atcgaagtca atcccctggc aggaatgaac cccgtccact
       61 ccgatctgcc catgctttca cgcatgaacg gccttgcctt cagtaccctg atggaaatga
      121 taatgaactc tgctaagaaa cgttacggac ttgcataatg acaaagaggt gcctgataat
      181 atacaatgaa ccggctcctg atgctctgcc ggacgaactc gatgtccttg accaggtcaa
      241 tttcataaat gagacactgc aaagccttgg ataccaggtt gagtgccgcg gaataaccga
      301 caatttcttc agtgagatcg gggagattgc cgaagaagaa tttgatttcg tattcaacct
      361 cgtggagtcg gtcggtaaaa aggcagagat actctatttt atccccggac ttctgaatat
      421 gaaccatatt ccctacaccg gctgtccggt ggaggctacg ttcattaccg ccagcaaggt
      481 gctggcgcga aagatcatga aagccaacgg cataccagtt gcaggaggtt acagggtatc
      541 agaagcctca cagcttgtcc ccgggaaaaa atatatcctc aaaccggtat gggaagacgg
      601 ctcactgggt attacagaag agtcggtctt tacctttaac ggcacgcctc ctgagattct
      661 caggggcaag aatgaccgcc actggttcat cgaggagttt attgagggac gggagttcaa
      721 tgtaagtgtc aaggccggac ctgacggacc tgagatgctg ccgcctgcag agatgctctt
      781 cgaggactat ccggatgaca tccccagaat agtaagttat aaggcgaagt gggtggcaga
      841 caccttccag tatgaaaaca gcagacggaa tttcccggga aacctgagcg aaaaactcat
      901 gaataacctg agagatgccg tcaacggctg ctggcatacc ttcggcctaa agggctatgc
      961 acgcgttgac atgagggttg acgccgatga aaatgtatat gtgcttgaag taaatgccaa
     1021 tccgtgcatc tcacctgaca gcggattcat ctcagctgca gttcatgccg gatatacgca
     1081 ccaggaaatt atcaggcaca tcataaatga cctgaatacc tgaacaatcc atgcctgaaa
     1141 tttcattcaa gataacaccg gccgacagac ctgcattcga gaagatactg gcttcatccg
     1201 gtttttttta cgattttgag atagaagtgg cgctttctct tgccgacgag acttttaccc
     1261 atggagaaga taaaagcgga tactactggg ttaagctggt ggaagacgga gaggtaatag
     1321 gatttgctaa tttcgggccc aatccttcat ctgtccactc atgggacatc tactggcttg
     1381 ccatacagga ggaatacagg aagaagcgct acggttccct gatcctgacc aagactgagg
     1441 atcgcatacg atcgctgggt ggaaagatag cgtggataga gacctcggga aggcctcttt
     1501 acgagcccac caggcacttc tacatcagga acggctatga actggaggct actctccgcg
     1561 agttctacgg cccgggtgac cctaaacttg tttaccggaa ggaattatga aaggtctctg
     1621 gtcaatactg ctgctgatag tctccaattc attcatgacg atagcctggt acggccatct
     1681 caagtaccag aacgtttcga agaatgccct gctggggctt ctgggcattg tgctggccag
     1741 ctggggaata gcgctgtttg aatatttctt ccaggtaccc gcaaacagga tcggctaccg
     1801 ggggaccggc ggtcctttct cactggttga gctcaagctt gtccaggaag tgatatcaat
     1861 atcagtattt gttgttttta ccctggtctt cttccgcact gagacattca agctcaacca
     1921 cctctttgcc ttcatgtgca tcctcggggc gatctacttc accttcaaag gctagcaagt
     1981 atatcacagg cttctcttat ctgcccgtcg ctcacatcga ggtgagtgac catccttatg
     2041 cttctttctc cggtcttcag ggcaagtatt cccctggctt ttatcttttc aaggaactct
     2101 gaggccgtat ttcccggctt cagggtgaag atgacgatgt tggttccgcc gtagttaacc
     2161 ttttcggtga ggctgcactt attcagcgct tccgccagaa acgcggcatt ccggtggtca
     2221 tcggccaggc gcaaaatgtt gtttttcagg gcatagatgc ctgccgcagc gatatagccg
     2281 gcctgcctca tggcgccacc gaacaacttc ctgatcctca ctccctgcct tatgatctcc
     2341 ctgtttccga tcagcaccga tccgaccggc gcgcccagtc ctttcgaaag acagattgaa
     2401 attgaatcga agagagcacc gtattcttcc ggtttcctgc cgtctgcagt catggcgttg
     2461 aagagcctgg caccgtcaag atggagtccc aggtgcctgc tgtcacacag ctccctgatg
     2521 cgcttcagat ccttgaaatc ccagcaggcg cctgcgcccc tgttgactgt gttttccacc
     2581 tccaccaggc gggtgaatgc cctgtgaggg tcatccggat tgttgatatg ctgttttaca
     2641 tcctctgcag taaaggtgcc gtttacagtg tcaatgaggg caacggaagc gcccgaatta
     2701 aatgccacac caccaccttc aaacaggtag atgtgggcca tcctgctgca gatgacctca
     2761 tcgccgggcc ttgtgtgtgc cttgatggct atctggttgg tcatggttcc cgaaggacag
     2821 aaaagggctc cctccatgcc aaacatttcc gctgccatcc gttcaagctc attgacttcc
     2881 gggtcttcgc cgaagatcat atcaccgaca ctggccttca tcatcctttc gagcattccg
     2941 gctgatggcc gggttacggt atcactgcgc aaatcaatca tcttcttctg aattgaagat
     3001 tctaagatag ctcattttta tccctccttc cgtgcaggct cgacacttca gtgaaaattc
     3061 aatatattta acatcattta gagggtgcgt gtcattatgg aggtaaagat cgaagctggc
     3121 tggaaagaaa aactcagcgg ggagtttgaa aaggagtact tcaggactct gacggaattt
     3181 gtccgggaag agtaccgtac caggcaggta ttcccgcctg ggaacaaaat cttcaacgca
     3241 tttgacctct gccctcttga cagggtaagg gttgtgatca taggacagga tccgtatcat
     3301 aattacgggc aggcacacgg actctgtttt tcagtaaatg acggaacaga gttccctccc
     3361 agcctggtga atattttcaa ggaactgaac cgcgacctcg gggtgcccgt acctaagtca
     3421 ggcaacctgg aacggtgggc gagccagggt gtgctactcc tcaacgctat acttactgtc
     3481 agggcacacc aggccctgtc acaccagaac aggggatggg aaaggttcac cgatgctgcc
     3541 ataagcgcac tgaaccgtga aagggaaaac ctggtattct tgctgtgggg cgcctatgca
     3601 cagaacaagg gggcatcaat tgacgagtca aagcacctgg tgctcaaaac tgtacacccc
     3661 tctcccctgt cagcatcacg cggctttttc ggctgctcgc acttcagcag gtgcaaccag
     3721 tggcttgttg ccagaggaat ggaaccaata caatggtaat catcccttca gtccgcgccc
     3781 gataatctca gccagcctgt cagtcttttc gtaaccaaca gacaatctta tcattcccgg
     3841 ctcaggatac ttcgttcccc atactgcctc cacaggcatc agaatggtat ggttgtcgcc
     3901 gagggtggga ataagtctta tttccggata aacagccgca ataaacctgt cgcggttctt
     3961 cctcttcgcc tctggcgaag ctcctgcaag atcaaaggtg atcatggcac cgtatccctt
     4021 ccctcccgtc agctctttcg ctgcttcatg ggtatcatgg ctttcaagcc cggggtaaag
     4081 cacgccggca accaggtctg acgacttcag cattgatgcc acagctgccg cattgtcaca
     4141 ctgttgcctg tagcggagct caaacgaagc catctgcgtc tgcagccggt atgcatcatc
     4201 aggcgacagc atgtgtccga catacttacg gtactctatt gcgctctgca tcaggtttgt
     4261 atcattgccg cagatgacac cggcagtcag attgccatga cctgacaggt acttcgtggc
     4321 actgtggatg accatgtcgg ccccgtcatc aaggggcttc cataataatg gtgtggcaaa
     4381 ggtgttgtca actatcactt tcgcaccgta ttttttccca agctcaatga ccctgcgtcc
     4441 ctctgcaacg atcagcagcg ggtttgagat ggtctcaaaa taaagaatat caggtcttat
     4501 gtcagatagt gcgttttcaa gctgccggta atcatacctg ccgtcaacgg gtctgaagaa
     4561 gcttacatcc atgccgcggc gcttttgcag cacgctgccg atgtatgata gtgtgccgcc
     4621 gtaaatctcc gaaaagaaga gccagttgct ttgtttccct gcctcctgga atattgaaag
     4681 ggctatgtca atggcggcca tgcctgactg tgccagcagc gcccagccgc acccctcaac
     4741 ctccatgact gcctcctcgg cagcgacaac cgtcgggttg cgataccgtg aatatatata
     4801 catatccggc tcccgctcat ggcctgtctc cgctgcaaaa gcgcccgagg ttgctgcagc
     4861 agtgggtaat tcaaagccgg aatcacgata taccggcgtc ctgacgctgt ttatctctct
     4921 ctttttcata ttctttcgcg aaatgttctg ttattgtatc ttttatattt ggcggcctga
     4981 agtcgcagta gaaaagcagc ctgtcggcca tcaggcctgt gatgaagatc acgagggctg
     5041 ctatgtcggt taccgggctg tcagggtata tcagcatgaa aaccattggc aatgccatcg
     5101 cccttgtata gtacagtgca cgtgtcaggc tgtctgcctc cgaagatctg taccgcatta
     5161 ccacggagat ggcggccagc attgaaagga caatgaatat tatcgtaggt tcaataaacc
     5221 aggttgcagc ataaagtcct gtaaagaaag cctggccgct gtggagtttc aggctgaacg
     5281 acctgtcggc agcaaaatat acattgtcta tggatatcag tgtcagcagc gccagcaagc
     5341 ccgttacaag cggcggcaga atgtccggtt tcatccactc caggagtgca acaaatgcaa
     5401 gcataagaac catggctatc tcgcggctca acggcgaaga gagaagattg agcactgccc
     5461 tccatgattt gccgggtacc cccagatgca caacagatac tgccatggca caaaccataa
     5521 ggatgaacgg cataaatccg ccggtaattg ttccccggag agccgagact atcaggatga
     5581 cataggctgt tattacgagc accgaaaata gagccaggct ccactcctcc ttcagcctgc
     5641 cggatgaacc tgaacttgtg ccctgtgttg tgagaatttc ttctacatct tcctccgatt
     5701 cattgccggc aggaataata aacggcttgt gattggtacc ggcattttta atgaaaacag
     5761 aaggttttat ccctgtgtca ggaaaccacg ccggaagagc accgtcgaat tcctcccttt
     5821 ctgtcatttt aagcgctccc gtggggcagg cggtgacgca ggccggctca acgccctgag
     5881 tggttctgtc agcgcagaaa tggcattttt caatgtatcc cagggcagga ttgattttcg
     5941 gggcaccata tgggcatctc catgtgcaat acccgcatcc catgcaacgg tctgtatgat
     6001 ggatgacaac tccgtcagga ctgatggtgt aggccagggc cgggcagcct gaggcgcagg
     6061 cgggttcggc acagtggttg caggccatgg agaggttgat gactc
//
LOCUS       DAOPXU010000139         6038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_16223_length_6038_cov_11.323751, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000139 DAOPXU010000000
VERSION     DAOPXU010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16223_length_6038_cov_11.323751"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..382
                     /locus_tag="PKK03_11045"
     CDS             137..382
                     /locus_tag="PKK03_11045"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNX84976.1"
                     /translation="MGQSYNIEICLGSSCFSRGNRETVQIIRDFLRKNHLTDKVVLKG
                     ARCMNRCSEGPFIIINGKTFTSIGVQDIEKILENELV"
     gene            411..2288
                     /locus_tag="PKK03_11050"
     CDS             411..2288
                     /locus_tag="PKK03_11050"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNX84977.1"
                     /translation="MKIPDQVIYINEDKCRNSYSCVRVCPVNAIEVKPERAHPVIIAD
                     RCIGCGLCFLACSPRAIEFRDSRTQVKQILQSGRPTAALIAPSIASEFDDITDYRKFV
                     GMIRSLGFTYVHEVSFGVDLVAYAYRKLFEESSGKYYITSNCPSIVEMIEKYHPNLVP
                     NLAPLVSPMVATALVTRGLYGDEVVNVFIGPCIDIKAEAERYGDRSLVEAVLTFIEIR
                     QLFEEYEIQEKTVKMSEFDPPFGNWGALYPYPAGILQAAGIKRDLVSSQVITASGAED
                     VKEAINDFDHHIDTIRHHFNLFFCQGCMLGPGMIRHDERFRRRSLVKQYAEKRVEALD
                     KKQWEKDMERWSELDLSHQFSVNDQRIPDPSPEAIIEVMKIIGKDNRSEELNCNACGY
                     GSCREFAATVAKGLAVPEMCHTYNLRNKQEYIETLRQTNKKLSETKKALKDSEEQAMR
                     EKDAAQDASDTMNSMLNKLPNGVVIVDNELKILQSNERFLEIIGEDAKAIAEIIPGLR
                     GADLKTLLPFNVYNIFSFVLRENESVISRDVQLEDRMFNISIFPIRQNRIAGAVIRDL
                     FSPEVQREEVINRVSDVIDKNLEMVQKIGFLLGEGASDTEKMLNSIVESFRQKKNATG
                     K"
     gene            2272..3486
                     /locus_tag="PKK03_11055"
     CDS             2272..3486
                     /locus_tag="PKK03_11055"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNX84978.1"
                     /translation="MQPESNATAAMERQFFIEVNSQQRNHHDERICGDVFLSVNVREE
                     NRIITVLSDGMGHGVKANILATLTSTMALNFTREHKEPDRIAEIIMNTLPVCSERKIS
                     YATFSIVDVESDGRANILEYDNPKCIVLRGSQPFEPDWKDVVLDKGRNAGKVLHKCTF
                     YPAKEDRIILLSDGVAQSGMGSAAWPLGWERDNVQQYASSLVASESSISASTLAAKIV
                     NMAYKHDNYEAKDDISCAILYFREPRKLLICTGPPYDEEKDKELADRVSGYKGKVILC
                     GGTTADIVARELNRKIVDELIFEDPDLPPESFMEGIDLVTEGILTLQKVTELVKGYKN
                     SVKLGKGPADKIVKMIMESDEINFIVGTRINVAHQDPTLPVDLEIRRTVVRRIARILE
                     DKWLKQVSVDYI"
     gene            complement(3663..4139)
                     /locus_tag="PKK03_11060"
     CDS             complement(3663..4139)
                     /locus_tag="PKK03_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNX84979.1"
                     /translation="MERIAEGSTPLKVSYVVIAARHNGGWLFVKHRRRGGWEMPAGHP
                     GPGEDTVEAAVRELTEETGAVSFTMEPVTYYSVDAGTGKQYGRLFCAEVELIGAHTDT
                     DEIEEVRIFRRLPRNLSLHEVMSFLYRVARQHGEGLKVPTPFGHRDFGSAGGGSSI"
     gene            4225..5403
                     /locus_tag="PKK03_11065"
     CDS             4225..5403
                     /locus_tag="PKK03_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNX84980.1"
                     /translation="MLFYLVLLVADLILWYILVRALKGRNWVLRIAVLLLKTAVTAAF
                     VTLFFRIVLYRGEFAEPANAFRQIQMGVVALLLISAAGSCLAVSITGRIAESLLKREM
                     KWTAMTNIVLFLLVTFFVADGYFRQRLDVRIVRQDVTVPGLDQALDGLKIALIGDIHL
                     SSWEGNYDRLEAAMKQAGREKPDLVLNTGDFITYGWQEFGMSDTILRKAGGLAGAFAV
                     EGNHDDGTYYPGYNSRYGELCREMVKRSIEKSGYTLLRDTAVIISHRGVRMAVAGVET
                     HGHHLDMRYGNFEKVLSPLPDSLFTILLLHDPESWLLSAVSGKIPQLTVAGHTHGFQA
                     ALPLGIWSPASLVHERWKGLYEFRGSHLYVTSGLGTMGMAARFFMPPEIVILTVKSGH
                     "
     gene            5477..>6038
                     /locus_tag="PKK03_11070"
     CDS             5477..>6038
                     /locus_tag="PKK03_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84981.1"
                     /translation="MRLNPLIVAAALLAALPSYPQKEALKAITLENSEAYMKFLSSDN
                     LEGRRTGSEGNNAAAEFIKAEALKLGLRPLPGQDDLFQQLRYLKIDIVPGESRITLSD
                     SCSGHILSAGIMPLMPPSDTVSLDGEVVFGGYGYMNSEEKYNDLAGISLKDKIVIIMT
                     RMPEIKGNGMPAPGTGISEMTEVRKLP"
BASE COUNT         1537 a         1466 c         1609 g         1426 t
ORIGIN      
        1 aaagctattt atttttattg ttatttcaaa aacattgtta ttttattaac aaatactggt
       61 tgaaaagaac tactgcatag tggaagcgtc gtaaaatatt gtacttttga gtgagtatga
      121 actaaagaaa agggtgttgg gacaatctta caacattgaa atctgccttg gaagttcttg
      181 tttttcaaga ggtaacaggg agactgttca gatcatcagg gattttctga ggaagaatca
      241 ccttacagac aaggtggtgc ttaagggtgc gcgctgcatg aaccgatgca gtgagggtcc
      301 gtttataatc atcaacggga agacctttac cagcattgga gtccaggata tagaaaagat
      361 actggaaaat gagcttgttt agtctgtgct taataacaaa ttaactgatt atgaagattc
      421 cggatcaggt aatatatata aacgaggata agtgccgtaa ctcttattca tgtgtaaggg
      481 tatgtccggt gaatgcaatc gaggtaaagc ccgagagggc gcatccggtg ataattgctg
      541 acaggtgcat agggtgcgga ctttgtttcc tggcatgttc tcccagggca attgagttca
      601 gggattcgcg gacccaggta aaacagatat tgcagtcggg ccgccctaca gcagccctga
      661 tagctcccag catcgcatcg gagtttgatg acataacgga ctaccgcaag tttgtaggga
      721 tgatacggag ccttggattc acctatgttc atgaggtatc ctttggcgtt gacctggtgg
      781 catatgctta caggaagctt ttcgaggagt caagcggcaa gtattatatc acttcaaact
      841 gtccttccat tgtggagatg atagagaagt atcatccgaa cctggttccc aaccttgcac
      901 cgctggtctc gcccatggtt gccacggcgc tggttacccg cggcctttat ggtgacgagg
      961 ttgtcaacgt tttcatcgga ccctgcattg acatcaaggc cgaagctgag cgttacggtg
     1021 acaggagcct cgtggaagcc gtcctgacat tcatcgagat acgtcagctc tttgaggagt
     1081 atgagataca ggaaaagacc gtcaagatgt ctgagtttga tcctcccttt ggcaactggg
     1141 gtgcactcta cccctaccct gccggcatcc ttcaggctgc aggcatcaag cgtgacctgg
     1201 tatcgagcca ggtgatcacc gcttcagggg cggaagacgt gaaggaggcg atcaacgact
     1261 tcgaccatca tattgacacc atcaggcacc acttcaatct cttcttctgc cagggttgca
     1321 tgctcggtcc gggcatgatc aggcatgacg aacggttcag gcgccgctcg cttgtgaaac
     1381 agtatgccga gaagcgggtt gaggcccttg ataaaaagca atgggaaaag gatatggagc
     1441 ggtggtcaga gctcgacctc tcgcaccagt tcagtgtcaa tgaccagcgg attccggatc
     1501 cgtcccctga agccatcatc gaggtgatga agatcatcgg caaggacaac cgttccgaag
     1561 agctcaactg caacgcttgc ggttacggct catgcaggga gtttgcagcc actgttgcaa
     1621 agggacttgc agtaccggag atgtgccaca cctacaacct gcgcaataag caggaatata
     1681 ttgaaacact gaggcagacc aacaagaaac tctcggagac taaaaaagcc ctgaaagatt
     1741 cggaggaaca agcaatgcgc gaaaaggatg ccgcacagga tgcctctgat accatgaaca
     1801 gcatgctcaa caagctgcca aacggagttg tgattgtaga caatgagctg aagatcctcc
     1861 agtcgaacga acgtttcctg gagatcatag gtgaggatgc aaaggctatt gcagagatca
     1921 tccctggcct tcgcggagcc gacctgaaga cactgctgcc atttaatgta tacaatatct
     1981 tttcctttgt gctccgtgag aatgaatcgg tgataagccg tgacgtgcag cttgaggacc
     2041 ggatgttcaa catctccatc ttccccatca ggcagaacag gattgccggg gcggtcatcc
     2101 gcgacctctt ctctcccgag gtacagcggg aggaggtgat caacagggtt tccgatgtca
     2161 ttgacaagaa cctggaaatg gttcagaaga tagggttcct cctgggtgaa ggagcctcag
     2221 atactgaaaa gatgctcaac tccattgtcg aatcgttccg ccagaagaag aatgcaaccg
     2281 gaaagtaatg ccacggcagc aatggagagg cagttcttca tcgaagtcaa cagccagcag
     2341 cgcaatcatc atgatgagcg tatctgcggt gatgttttcc tgtcagtaaa cgtgcgtgag
     2401 gagaaccgca ttataacggt tctctctgac ggcatggggc atggggtaaa ggccaacatc
     2461 ctggccaccc tgacctctac catggcactc aacttcaccc gcgagcacaa ggagcccgac
     2521 cgtattgcag agattatcat gaataccctc ccggtatgtt ccgagaggaa gatcagctat
     2581 gcaacgtttt caattgttga cgttgagagt gacggaaggg ccaacatact tgagtatgac
     2641 aacccgaagt gcatagtcct caggggcagc cagccctttg agcccgactg gaaggatgtg
     2701 gtacttgaca aagggagaaa tgccggtaag gtcctgcaca aatgcacctt ttatccggcc
     2761 aaggaggacc ggataatact gctctccgat ggcgttgcgc agagcggtat gggatcagcg
     2821 gcctggccgt tgggatggga gcgcgacaat gtacagcaat atgcctcctc ccttgttgcc
     2881 agtgaatcct ccatatcggc atccacgctg gcggccaaga ttgtcaacat ggcctacaag
     2941 catgacaatt acgaagcaaa ggatgacatc tcatgtgcaa tactttactt cagggaacct
     3001 cgaaagctgc tgatatgtac cgggcctccc tacgatgagg agaaggataa ggaactggct
     3061 gacagggtat ctggatacaa gggcaaggtg atattatgcg gtggcaccac cgccgatata
     3121 gttgcacggg aactgaaccg taagatcgtc gatgagctga tcttcgaaga ccccgacctg
     3181 ccgccggaga gcttcatgga gggtatcgac cttgttaccg aggggatact caccctgcaa
     3241 aaggtgacag agctggtcaa gggttacaaa aacagtgtga agctgggaaa aggaccggct
     3301 gacaagatcg taaaaatgat tatggagagc gacgagatca acttcatagt cggcacacgc
     3361 ataaatgtgg ctcatcagga tcccacgctt cctgttgacc ttgagatacg tcgtacggtt
     3421 gtcaggcgca tagcccgtat cctggaggac aaatggctga agcaggtcag tgttgactat
     3481 atctgaggga tttgcgattt gcatcccgat ccgccggctg gcggatacgg gattgagcct
     3541 gctccccgac gctccgcgta cgggactcct cctttgtcac agcctcaaat gtgcgaattg
     3601 ggcaatagac agttgtgttg acaatgtatt aatgaattgt aaatcaatgt aatatctgac
     3661 cctcagattg aggaaccgcc gccggcggat ccgaaatccc gatgcccgaa gggtgtcggg
     3721 accttcagac cttcgccatg ttgtctcgct accctgtaca ggaatgacat cacttcgtga
     3781 agggagaggt ttcgcggcag gcgcctgaag atccgtacct cctctatctc gtcagtgtca
     3841 gtgtgtgcgc caatcagctc aacttcagcg caaaagagcc tgccgtattg ttttccggtc
     3901 cctgcgtcaa ccgaataata tgttaccggc tccatggtga agctgaccgc tcccgtttcc
     3961 tccgtcagct cacgcactgc cgcctcaacc gtatcctctc ccggccccgg atgaccggca
     4021 ggcatctccc agcctcccct cctccggtgc ttcacaaaca gccatccgcc attatgacgg
     4081 gcagcaatta caacgtatga tactttcagc ggcgtgctgc cttccgctat tcgttccacg
     4141 gtcaccatac ggtaaaatta aatcaaaaag agaattatgt accggattat cccttaaata
     4201 ttacttttgt ccaaaactta gcagatgttg ttctacctgg ttctactggt tgccgacctg
     4261 attctctggt atatcctggt cagggcactg aagggaagaa actgggtgct ccgtattgct
     4321 gttttgttgc tcaagacagc agtgacagcc gcctttgtga ctctcttctt caggatagta
     4381 ctgtaccggg gtgagtttgc cgaaccggca aatgccttca ggcagattca gatgggggtt
     4441 gtggccctgc tgctgatatc ggcagccgga tcatgcctgg cagtgtcaat aacaggacgt
     4501 atagctgagt ctctcctgaa gcgggagatg aaatggactg cgatgaccaa tattgtccta
     4561 ttcctccttg taacgttttt tgttgctgac ggttacttcc gccagaggct tgatgtccgg
     4621 attgtgaggc aggatgtcac agtcccggga cttgatcagg ctcttgacgg actcaagata
     4681 gctctcatag gcgatataca tctctcctca tgggaaggaa actacgaccg gcttgaagcg
     4741 gccatgaagc aggccggcag ggagaaaccg gatctcgtgc tcaacacagg cgatttcatt
     4801 acttacggct ggcaggagtt cggcatgtct gacacgatac tccgtaaggc aggaggcctg
     4861 gcaggagcat tcgccgttga aggaaaccat gacgacggaa catattatcc cggctacaac
     4921 agcaggtacg gagagctgtg ccgtgagatg gttaagagga gcattgaaaa gtcgggctac
     4981 acccttctga gagatactgc agtcatcatc agccacaggg gagtgagaat ggcagttgcc
     5041 ggagtagaga ctcatggtca ccatcttgat atgagatacg gtaattttga gaaggtactg
     5101 agcccgttgc ctgattctct gttcactata ttgctgctgc acgatccgga atcatggctt
     5161 ctgtcggcgg tttcaggaaa aattccgcag ctcactgttg cagggcacac tcacgggttt
     5221 caggcggccc ttccccttgg catctggtcg ccggcatcgc tggtccatga gaggtggaaa
     5281 gggctgtatg aattcagggg cagtcatctt tatgtcacct ccggccttgg cacaatggga
     5341 atggcagcaa ggtttttcat gccccctgag atcgttattc ttaccgtgaa atccggtcat
     5401 taatttatca gggtataaat aacaaccgat ctctcattta tgtaactttg cagtttattt
     5461 ataaactgtt tacagcatga gacttaaccc tctgatcgta gctgccgccc tgttggcagc
     5521 cctgccatct tacccgcaga aggaagctct gaaagctatc acccttgaaa actcggaagc
     5581 atacatgaag ttcctctctt ctgacaacct tgaggggaga aggacaggga gcgaagggaa
     5641 caacgcagct gcagagttca ttaaggctga agccctgaag ctgggtttgc gcccgcttcc
     5701 cggccaggat gatctgtttc agcaactcag gtacttaaag atcgacatag tccctggcga
     5761 atcacgcata acactcagtg acagctgttc gggtcatatt ctctccgccg gtatcatgcc
     5821 cctgatgccc ccttctgaca ctgttagcct tgacggcgaa gtggtttttg gaggttacgg
     5881 atacatgaac agtgaggaaa agtacaatga cctggcgggg atcagcctca aggacaagat
     5941 tgtcataatt atgacaagga tgccggagat caaggggaac ggaatgccgg caccgggcac
     6001 tggcatcagc gagatgaccg aggtcaggaa gcttccct
//
LOCUS       DAOPXU010000140         5868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_16897_length_5868_cov_10.667125, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000140 DAOPXU010000000
VERSION     DAOPXU010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16897_length_5868_cov_10.667125"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PKK03_11075"
     CDS             <1..566
                     /locus_tag="PKK03_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNX84982.1"
                     /translation="RVAESVPEMPVYYYHIPVLTGGFFNMIDFVREVAPRVPNFAGIK
                     YTHEDFMDFLSCLNYDKGRYDMLWGRDENFLSALVLGARGAVGSTYNYAAPLYHRMIE
                     AFERNDQVTARAMQEKSIDMITLLAKYGGIATGKAYMRFIGLDCGEFRLPVRNMSVAD
                     YQAFVKDVRELGMDDLFSKASGMARFR"
     gene            575..1759
                     /locus_tag="PKK03_11080"
     CDS             575..1759
                     /locus_tag="PKK03_11080"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HNX84983.1"
                     /translation="MNTQLQSVAERYRNALLNDIIPFWMKYSPDRERGGYFTCLDRNG
                     KVYDTDKFIWLQGREVWMFSHLFNNVEKRQEWLETALSGAAFLEKYGSDRDGNFYFSL
                     TREGKPLVHPCNIFSDCFAAMAFGELFRATGNSRYGEIALNTFRNILGRSGNPKGNWS
                     KVYPGTRPLKGFSLPMILSNLSGIIAPLLDAGTVERVREECIHEVMDVFNSKEYGLIL
                     ENVAPDGSFSDSFEGRLLNPGHVTEAMWFMMDLSEIRGDQKLAARATEITLQTLSKGW
                     DEKHGGIFYFLDVKGYPPQQLEWDQKLWWVHIETLISLAKGYRLTGDRRCLDWFEKVD
                     AYTWSHFPDPESGEWYGYLNRRGEVLLPLKGGKWKGCFHIPRGLLQVWKTLSDVEPVK
                     TA"
     gene            1762..3003
                     /locus_tag="PKK03_11085"
     CDS             1762..3003
                     /locus_tag="PKK03_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX84984.1"
                     /translation="MKESVAERRYAWVVVALLWVVALLNYMDRQMLSTMKNAMMVDIV
                     ELEKAENFGRLMAVFLWIYGLMSPVAGIIGDRLNRKWLIVGSLFVWSGVTFAMGYTHD
                     YGVLYALRAIMGVSEALYIPAGLSLIADYHQGSTRSLAVGIHMTGLYMGQALGGFGAT
                     IADSLSWQKTFQYFGIVGIIYSLVLILLLREYKYRKKVKKVSPGVSGFRSAASGLSVL
                     FGMISFWMILFYFAVPSIPGWAVKNWIPTLFSSSLGIEMPLAGPMSTIIIAFSSLVGV
                     IFGGILSDRWILKNIRGRIYTGAIGLSLNIPSLLLIGLGHSYLWIIGGAVLFGIGYGM
                     FDANNMPILCQFVTPRLRATGYGLMNMAGVFAGAFITRLLGKSMDSGNLARDMALLAI
                     PVLLAVILQLAILRPESANKE"
     gene            complement(3037..3405)
                     /locus_tag="PKK03_11090"
     CDS             complement(3037..3405)
                     /locus_tag="PKK03_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84985.1"
                     /translation="MKGNVIIMVTPRVCPKVLPGRLLTLNIKGTKNRPRTVNAIPPHS
                     SCFQAKIRRAIKANDGIRWRKKPARFCQNELSSENASNANRLMKIIARMPRILGVHHK
                     NRLCGFIPQFLEVNTKVLHL"
     gene            3543..4262
                     /locus_tag="PKK03_11095"
     CDS             3543..4262
                     /locus_tag="PKK03_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84986.1"
                     /translation="MKKPVGIIFMTLALFFTGRASGQGFVETVEEGGMSAMYGNECVV
                     TFQSGEEITGKFAGGVYVINGLTKIKVKLETGENAKFSPDQVVSLKVKPSKLMKLSMI
                     SDAGSSIKEFADSDLNGIVNREWVIFETATTPAQKETRRILQLLNPGFDSKIKVFAEP
                     GAKTGGLSIGGLQVTGGEDRVYLFVKEGGKAFKVKKGSYRENFQELYSDCPQMLEQFQ
                     GEKIRWDDVALHVYAYDQLCR"
     gene            4528..4914
                     /locus_tag="PKK03_11100"
     CDS             4528..4914
                     /locus_tag="PKK03_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNX84987.1"
                     /translation="MKTMQLTRAEEQVMQILWDMGEGLVKDIRDRFDEPRPARNTVST
                     VVRILEKKGFVDHKSYGNVYLYFPVVSRDEYSRHQLFGLLEGYFDNSFPAMASFFARE
                     KDLTMAEMEELIKETRKELSDSKLQE"
     gene            4914..>5868
                     /locus_tag="PKK03_11105"
     CDS             4914..>5868
                     /locus_tag="PKK03_11105"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HNX84988.1"
                     /translation="MEAVGFYLLRSAVWITGFALVYLLFLRNERFFLLNRIYLIIGIM
                     ASVVLPLFTVRYAVEVSVAEAAAEAGPATAALSGGSSWQNILAIALFAVWLAGAGTML
                     FRYVMQVLPVLRAAGDADRSSGYPVKVLRSPEFPGSFSLFSYVVVNPSVTETETREIL
                     IHEMVHIRQKHWFDLVLSSLLCMVQWFNPAVWIYSRFIRQNHEYLADEEALQRSSDPA
                     VYRAVLLNQIAGSPVIDLGNFFSYSLNKKRFQMMKNKISSPYRKLRLLLILPVAALVL
                     YAFAEPRYTVSPEEQGLTASAAVTADMTKNVSGVVTDEKGGP"
BASE COUNT         1456 a         1285 c         1617 g         1510 t
ORIGIN      
        1 tcagggtggc ggaatcagtt cctgaaatgc ccgtttatta ctatcatatc ccggtgctta
       61 ccggtggatt tttcaacatg atcgactttg tcagggaggt ggcgccgcgt gtgcccaact
      121 ttgcaggcat caagtataca cacgaagact ttatggattt cctgagttgc ctgaattatg
      181 acaaaggcag gtatgacatg ctgtggggca gagacgaaaa cttcctttct gcgctggtcc
      241 ttggagcccg gggtgctgtc ggaagcacat acaactatgc cgctccactc tatcacagga
      301 tgattgaggc ctttgaaagg aatgatcagg tcactgcccg cgcaatgcag gagaaatcaa
      361 ttgacatgat cactttgctg gccaaatacg gaggaattgc cacgggaaaa gcctatatga
      421 ggttcatagg ccttgactgc ggcgagttca ggctgccggt aaggaatatg tcagtggctg
      481 actaccaggc attcgttaag gatgtcaggg aactgggaat ggatgatctg ttttcaaagg
      541 catcaggaat ggccagattc aggtaaagaa gactatgaat actcaattac agtcagtagc
      601 tgagcggtac aggaatgcgc tgttgaatga catcattcca ttctggatga agtactcgcc
      661 tgatcgtgaa cggggaggct atttcacatg ccttgaccgt aacggaaagg tatatgacac
      721 ggacaagttc atctggctcc agggaagaga ggtatggatg ttcagtcacc tcttcaacaa
      781 cgttgagaaa cgacaggaat ggctggaaac ggcactgtcc ggagcagcct tccttgagaa
      841 atacggaagt gaccgtgatg gcaactttta tttctctctg acccgggaag ggaagcctct
      901 cgtccatccc tgcaatattt tctctgactg tttcgctgca atggcatttg gtgaattgtt
      961 cagggcaaca ggcaacagca ggtacggtga gattgcattg aatacattcc ggaacattct
     1021 cggccgttcc ggtaatccta aaggcaactg gtcaaaagta tatccgggca cccggccgct
     1081 gaagggcttc tcccttccga tgattctcag caatctctcg gggatcatag caccgcttct
     1141 tgatgccgga actgttgaaa gggtaaggga ggagtgtata catgaggtaa tggatgtctt
     1201 caacagcaag gaatatggcc tgatcctgga gaacgtggct cctgacggat cattttctga
     1261 ttcgtttgaa ggccggctgc tgaatcccgg acacgtcaca gaagccatgt ggttcatgat
     1321 ggacctctcc gagatcaggg gagatcagaa gcttgcagcc agggccaccg aaatcaccct
     1381 gcagacgtta agtaaggggt gggatgagaa gcatggtggc atattctatt tcctggacgt
     1441 aaaaggatat cctccccagc aactggagtg ggatcagaag ctctggtggg tgcatatcga
     1501 aactcttatc tcactggcaa aggggtacag gctgacaggt gacaggcgct gcctcgactg
     1561 gtttgagaag gttgatgcct atacatggag tcacttcccc gaccctgaat ccggagagtg
     1621 gtacggttac ctgaaccgga ggggagaagt gctcctgccg ctcaagggcg gaaagtggaa
     1681 ggggtgtttc cacatcccca gaggattgct gcaggtatgg aagacactca gtgatgttga
     1741 accggtaaaa actgcataat gatgaaagag agcgttgcag aaagaaggta tgcctgggtt
     1801 gtggtggcgc tgctctgggt ggttgctttg ctcaactata tggaccgcca gatgctttcc
     1861 accatgaaga atgccatgat ggttgacatc gtggaattgg aaaaggctga gaatttcggc
     1921 aggctgatgg cggttttcct ctggatctac gggctgatga gtcccgtagc gggaatcatt
     1981 ggcgacaggc tcaacaggaa atggctgatc gtcggcagcc tcttcgtatg gtcaggggtc
     2041 actttcgcaa tgggctatac ccatgactat ggggttctct atgccctgcg ggcgataatg
     2101 ggtgtcagtg aggcgcttta cataccagcc gggctttccc ttattgccga ttatcaccag
     2161 ggctcaacac gatcactggc ggtgggaata catatgacgg ggctatatat gggacaggcc
     2221 ctgggtggtt tcggggccac catagctgac agcctgtcat ggcagaaaac atttcaatac
     2281 ttcggtattg tgggcataat atacagcctg gtgctgattt tgctgcttcg cgaatacaaa
     2341 tacaggaaga aagtaaaaaa ggtctcaccg ggtgtctccg gattcaggtc agcagcttcg
     2401 gggctttccg ttctgttcgg gatgataagt ttctggatga tcctctttta tttcgccgta
     2461 ccgagcatac caggctgggc tgtcaagaac tggatcccga cgctcttctc atcaagtctt
     2521 ggcatagaga tgccgttagc cgggccgatg tcaaccataa ttattgcatt ctcctcactg
     2581 gttggtgtga tcttcggggg catactgtct gacaggtgga tactgaagaa cattcgcggt
     2641 cgcatttata caggtgccat aggcctgtca ctcaacattc cgtcacttct gctgatcgga
     2701 ctcgggcaca gctacctgtg gatcataggc ggcgcagtac ttttcggaat cgggtatggc
     2761 atgtttgatg ccaataacat gccgatcctc tgccagtttg ttacgccgcg cctgagggcg
     2821 acgggttatg gcctgatgaa tatggcaggc gtatttgctg gagctttcat aaccaggctt
     2881 ctgggaaaat cgatggattc aggtaacctg gcacgtgata tggctctgct ggcaatcccg
     2941 gttctgcttg ccgtcatact tcagctggcc attctgaggc ccgaaagcgc aaataaagag
     3001 taagagcaga ctgaatgaaa taaaattgac atcccgttac aggtggagta cctttgtgtt
     3061 aacttctaaa aactgaggta tgaaaccaca taaccgattt ttatggtgga cccctaggat
     3121 cctgggcatt cttgcaatta ttttcataag cctgttcgca tttgatgcat tttctgagga
     3181 aagttcattc tggcagaacc tggcaggctt ttttctccac cttatcccgt catttgcttt
     3241 gattgccctt ctgatttttg cctggaaaca ggaactgtgg ggagggattg catttacagt
     3301 tctgggcctg ttttttgtcc ctttgatatt caatgtcaat aaccggcccg gcaacacctt
     3361 cgggcagact ctgggagtta ccatgatgat tacattgcct ttcattgtca taggaatact
     3421 ttttattgtc agtcattaca tgaataaaag gcacgggacc ggtgaacaac agccttgatt
     3481 cagtgttcat tgatccggaa gagacacagt atcttaaacc attactaaaa aaaatccgta
     3541 tcatgaaaaa accagttgga atcattttca tgaccctggc gctgtttttt acaggcaggg
     3601 cttcaggtca gggttttgtt gagacagtag aggaaggggg catgtcggct atgtatggca
     3661 acgaatgcgt ggtgaccttt caatctggcg aagagatcac cggcaagttt gcaggtgggg
     3721 tttatgtgat aaatggtttg accaaaatca aggttaagct tgaaaccggg gagaatgcta
     3781 aattctcgcc tgatcaggtt gtctcactga aggtgaaacc ctcgaagctc atgaagctta
     3841 gcatgatcag tgatgccgga agctccatca aggagtttgc tgacagtgac ctgaacggaa
     3901 ttgtaaatcg cgagtgggtg atatttgaaa cggcaacaac tccggctcag aaggaaacca
     3961 ggagaatcct ccagctgctg aatcccggct ttgacagcaa aatcaaggtg ttcgccgaac
     4021 caggcgcaaa aacaggcggg ctaagtatag gcggactcca ggttacggga ggcgaagaca
     4081 gggtgtatct ctttgtgaag gaagggggga aagctttcaa ggtaaagaag ggctcctatc
     4141 gggagaattt ccaggagcta tattcagact gtccgcagat gctcgaacag ttccagggtg
     4201 agaagatccg ctgggatgat gtggcactgc atgtttatgc atacgatcag ctttgcagat
     4261 aaggactctt ttcgcttcct gccggggaaa cctgtcaaag gcggctcttt agcggagaac
     4321 aataccagcc cctaccagtg aaaatcacgc aaagcggctc tgccccggaa aatcggcaca
     4381 cgactgacca cgattatctg gattgtaaaa atctgtaata ctgcagatta gcctatagac
     4441 taaaaaaata gtcaaaaaaa gtgcataatt agttgcatga ctaagaaatt agtcatatat
     4501 ttgcattgaa ggattgacag aaaagttatg aagacaatgc aacttacgag ggcagaggag
     4561 caggtgatgc agattttgtg ggatatgggt gaaggactgg tcaaggatat ccgcgaccgg
     4621 tttgacgaac ccaggcctgc gaggaacact gtatcgactg tggtaagaat tcttgagaaa
     4681 aagggatttg tggatcataa gtcgtacggg aacgtctatt tgtattttcc ggttgtttcc
     4741 cgtgatgaat attcgcgcca tcagctcttt ggccttcttg aggggtattt tgacaactcg
     4801 ttccctgcta tggcatcatt ctttgcacgt gaaaaggatc ttaccatggc ggagatggaa
     4861 gagctgatca aagagacacg gaaggaatta tcggacagta aactgcaaga gtaatggaag
     4921 cggtgggttt ttaccttctc aggtcagcag tatggataac ggggtttgcc cttgtttatc
     4981 tgctctttct caggaatgag aggttttttc ttctgaaccg tatatacctt attattggta
     5041 tcatggcttc ggtcgtcctt ccgctgttca cagtgcggta tgcggttgag gtgtctgtcg
     5101 ctgaggcggc tgccgaagcc gggccggcaa cagcagcgct gagtggcggc agttcctggc
     5161 agaatatact ggcgattgcg ttgtttgccg tctggcttgc cggagcgggg accatgcttt
     5221 tcaggtatgt catgcaggtg ttgcctgtgc tcagggcagc gggtgatgct gaccggagct
     5281 ccggttatcc ggtgaaggtg ttgcgttcgc ccgagtttcc cggatcattt tcattattct
     5341 cctatgttgt tgtcaatcct tcagttacag agaccgagac aagggagatt ttgattcatg
     5401 agatggtgca tatcaggcag aagcactggt ttgacctggt gctttcatcg cttctgtgca
     5461 tggttcagtg gttcaatcct gccgtatgga tctattccag gttcatcagg cagaaccatg
     5521 agtatctggc tgatgaggag gcgctgcagc gttcctccga tccggccgtt tacagggccg
     5581 tgctgcttaa ccagatcgcc ggctctccgg taatcgacct gggcaacttt ttcagttatt
     5641 cactaaacaa aaaacgattt caaatgatga aaaataaaat cagttcgcca taccgtaagc
     5701 tcaggctgtt actgatcctg cctgtcgccg cactggtact ctatgcattt gcagagccca
     5761 ggtacaccgt cagcccggag gagcagggat taactgcttc ggccgcggtc acggcagaca
     5821 tgacaaaaaa tgtcagcggg gtggttacgg atgaaaaggg tggcccgc
//
LOCUS       DAOPXU010000141         5864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_16917_length_5864_cov_11.886039, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000141 DAOPXU010000000
VERSION     DAOPXU010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16917_length_5864_cov_11.886039"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2360
                     /gene="leuS"
                     /locus_tag="PKK03_11110"
     CDS             <1..2360
                     /gene="leuS"
                     /locus_tag="PKK03_11110"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNX84989.1"
                     /translation="TTNKNCDTFRRQIKELGLSYDWDREINTTDPKYFKWTQWIFIKL
                     YNTWYDHNANRGRDINEMPIPLDVLKAGDEARKKYIDSKRLAYYDNAQIWYCPSCRIV
                     CANEEVLTDGSHEKCGNRVVRRNLKQWMLRIPYYAERLLSGLDSLDWPEGIKEMQRNW
                     IGRSTGAEVDFKIDGTRERITVYTTRPDTLFGATYMVLSPEHPLVEKITNPEMVSQVK
                     AYVAAASLKSDLDRTDLAKDKTGVFTGTYAVNPVSNKKIPVWVADYVLMGYGTGAIMA
                     VPAHDERDFEFAKKFGIEITCILDPAVTDTEQRERIIAGNECWSGDGSYINSSNKELN
                     ISLDGLNVEEGIATITTWLESRNLGIRRVNYKLRDWLFSRQRYWGEPFPVIHWEDGTI
                     GLLDEKDLPLTLPELEVYEPGDSGESPLSNATDWLWVTDKNGRRGKRETNTMPQWAGS
                     CWYYLRFIDPHNDKAIFDPEKEKYWMPVDLYIGGAEHAVLHLLYARFWHKVLFDLGVV
                     STDEPFMKLFNQGMILAFAYETATGAKVSSDLITEKDGKFFITESGEEVRQIVAKMSK
                     SLKNVINPDDVVKNYGADTLRLFEMFLGPLEATKPWDEKGIRGPHNFLSRVHRLIANT
                     ENITDGDEDTEVLKTLHKTIKKVTEDIEGLRFNTAISAMMIFMNTALKKEKITRTSAS
                     VFIRLLAPFAPHLAEELWQLTGHRGSLTYEPWPVAEEKFLKEHVFECPVSVNGKMRFK
                     IELPVNLPVEEVKKIVLADERAQKWIGTGTPKVIVVPNRIVNIVV"
     gene            2357..3655
                     /locus_tag="PKK03_11115"
     CDS             2357..3655
                     /locus_tag="PKK03_11115"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNX84990.1"
                     /translation="MRFRLIPAALIVAAMLTSVSCVRSGAAENTPTGDVARPVTSETP
                     SDTASVAGMPADSTVMDSGQNTLFGIPVDSYNVKTEKVRRNQFISSILAAQGITWNDI
                     EKLLGDNKETFDPRRVRTGSTYSVLTTKDTLSKPEYIIYQHDPRVAYVFSFKDTLAIY
                     RHDAEIKRHLRYSSGTISTSLWEAAQENNLNPNLSAELSEIYAWTIDFFGLQKGDRFK
                     VIYEEDFIGDESVGIRRVHAAEFEHAGSTIYAIPYIQDSVMSFFDTTGASLRKAFLKA
                     PLRYSRISSRFSGGRMHPILKIVRAHHGVDYAAPLGTPVLAIGDGRVTSTAYENGSGR
                     IVRITHNSVYSTAYMHLSRFGPGISSGVYVKQGQVIGYVGSSGLSTGPHLDFRFYRNG
                     SPIDPLKVEAPPVNPVAPEAMEEFNRVAEGYINLLATIKY"
     gene            complement(3736..4305)
                     /gene="purN"
                     /locus_tag="PKK03_11120"
     CDS             complement(3736..4305)
                     /gene="purN"
                     /locus_tag="PKK03_11120"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNX84991.1"
                     /translation="MKNIAILASGAGTNAENIIRYFSNEKKAKVTLVLSNKPQAMVLK
                     RAAALGVDAFFFDRSQFYTTGEVLMMLRRRETHLVVLAGFLWLVPGDVIGAYRGRIVN
                     IHPALLPGFGGKGMYGDRVHRAVLDAGCTESGITIHYVNEHYDSGDIIFQARCPVLPG
                     DDADSLAARVHKLEYEHYPRVIEKLVEQS"
     gene            4491..4727
                     /locus_tag="PKK03_11125"
     CDS             4491..4727
                     /locus_tag="PKK03_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNX84992.1"
                     /translation="MSDISTRVKKIIVEKLGVDEAEVTPEAHFTNDLGADSLDTVELI
                     MEFEKEFNIGIPDDQAESISTVGEAIKYIEENAK"
     gene            4748..>5864
                     /gene="fabF"
                     /locus_tag="PKK03_11130"
     CDS             4748..>5864
                     /gene="fabF"
                     /locus_tag="PKK03_11130"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNX84993.1"
                     /translation="MRRVVVTGMGALTPIGNNLQDFWKSLVDGVSGAGLITHFDTSKF
                     KTRFACEIKNYDASRYFDRKESRKMDRYTQYAHVAADEAVKDAGLDPEATDKDRSGVI
                     WASGIGGIETILEEVKGFVMGDGTPRFSPFFIPKMISDIAAGMLSIKYGFRGPNFATV
                     SACASSTNAIIDAYYYILLGKADLFIAGGSEASINPPGIGGFNAMQAMSTNNEEYATA
                     SRPFDVTRDGFVIGEGAGALILEELDHARARGARIYAEVIGSGLTADAYHITAPHPDG
                     MGAVNVMKQAINESGLKPSDVDYINVHGTATPLGDIAEIKAILALFGEHAYKLNISAT
                     KSMTGHLLGAAGVVEAIATTMAVVHDIIPPTINFRNPD"
BASE COUNT         1528 a         1530 c         1503 g         1303 t
ORIGIN      
        1 taaccacaaa caaaaactgc gacaccttcc gccgccagat aaaggaactg ggcctgagct
       61 acgactggga ccgcgagata aataccactg acccgaaata cttcaaatgg acacagtgga
      121 tcttcataaa gctctataac acctggtatg atcacaatgc caaccgcgga cgtgacataa
      181 atgaaatgcc cataccactt gacgtcctca aggcaggtga tgaggcaagg aagaaataca
      241 tcgactcgaa gaggctcgcg tactatgaca atgcccagat ctggtactgc ccctcatgcc
      301 gcattgtctg cgccaacgag gaggtgctga ccgacggctc gcacgagaag tgcggaaaca
      361 gggtggtgcg aagaaacctc aaacagtgga tgctccgtat cccttactat gccgagagac
      421 tgctcagcgg actcgacagc ctcgattggc ccgaggggat caaggagatg caacgtaact
      481 ggataggccg ctccaccggc gccgaggttg actttaaaat tgacggcacc cgggaacgga
      541 taacagtcta taccacccgt ccggatacac tcttcggggc aacctatatg gtgctctcac
      601 cagagcaccc gctcgtggaa aagatcacaa acccggagat ggtatcgcag gtgaaggcat
      661 atgtggcagc agcctccctg aagtcagacc ttgaccgtac tgacctggcc aaggacaaaa
      721 ccggtgtctt caccggaaca tacgccgtca accctgtcag caacaagaaa ataccggtgt
      781 gggtggcaga ctacgtgctc atgggctacg gaaccggcgc catcatggca gtgccggctc
      841 acgacgagcg cgactttgaa tttgcaaaga agttcgggat agagataaca tgcatccttg
      901 atccggccgt gactgacact gagcagcggg agaggatcat cgccggtaac gagtgctgga
      961 gcggcgacgg cagttatatc aactcatcaa acaaggaact gaatatcagc cttgacggac
     1021 tgaacgtcga agagggaata gccaccatca ccacatggct cgagagcagg aacctgggaa
     1081 tccgtcgcgt caactacaag ctgcgcgact ggctcttcag caggcagcgc tactggggcg
     1141 aacccttccc cgttatccac tgggaagacg gaaccatcgg ccttctcgac gagaaggatc
     1201 tgcctctgac cctcccggag ctcgaggtct acgaaccggg cgacagcggc gaatcgcctc
     1261 tctccaacgc aaccgactgg ctgtgggtca ctgacaaaaa cggtcgtcgc ggcaagcgcg
     1321 agaccaatac catgccacaa tgggccgggt catgctggta ttacctgaga ttcatagacc
     1381 cgcataatga caaggctatc tttgaccctg agaaggaaaa atactggatg ccggtcgacc
     1441 tctatatagg aggagctgaa catgccgtgc tgcacctgct ctatgcccgc ttctggcaca
     1501 aggtgctctt tgatctcggg gtggtatcaa ccgatgagcc cttcatgaag ctgttcaacc
     1561 aggggatgat acttgcattt gcctacgaaa cagctaccgg cgccaaggtg tcatcagacc
     1621 tcatcactga gaaagacggc aaattcttta tcacggaaag cggtgaagag gtgaggcaga
     1681 tagttgccaa aatgtctaaa agcctcaaga atgtgatcaa tcccgacgat gtggtgaaga
     1741 actacggtgc agacacactt cgcctgttcg agatgttcct gggccctctg gaagcaacaa
     1801 agccttggga cgagaagggg atcaggggtc cgcacaactt cctgtcaaga gtccaccgcc
     1861 tcatagccaa cactgagaac atcaccgatg gcgatgaaga cactgaggtc ctgaaaaccc
     1921 tgcataaaac aattaaaaag gtcacagaag acatcgaagg cctcaggttc aacacggcca
     1981 tctcggcaat gatgatcttc atgaacaccg cgctgaaaaa ggagaagatc acccgcacct
     2041 cagcctctgt cttcataagg ctccttgccc cctttgcccc tcaccttgca gaagaactgt
     2101 ggcagctcac cgggcacagg ggatcattaa cttatgagcc atggcccgtg gccgaagaaa
     2161 aattccttaa agagcatgtg tttgaatgcc ctgtctcggt caacgggaag atgaggttta
     2221 agatagagct gcccgtaaat ctccccgttg aggaggtgaa aaagatcgtg ctcgctgatg
     2281 agagggccca aaaatggatt ggcacgggaa ctccaaaagt catagtggta cccaaccgta
     2341 ttgtaaatat agttgtatga gattcagact tattcccgcg gccctgattg ttgccgcaat
     2401 gctgacatcg gtttcttgcg ttcgcagcgg agctgctgaa aatacaccta cgggagatgt
     2461 ggcccggccg gtcacttctg aaacaccatc tgataccgct tctgttgcag gtatgcctgc
     2521 cgattcaacg gtaatggatt caggtcagaa tactcttttc gggatacccg ttgactccta
     2581 taatgtcaaa acggagaagg ttagaaggaa ccagttcatc tcttcaatcc tggcagccca
     2641 ggggattacc tggaatgaca ttgaaaagct gctcggcgac aacaaagaaa cttttgatcc
     2701 gcgcagggtg cggaccggca gtacttattc ggtactgact acaaaagaca cgctgagcaa
     2761 accagaatac attatctatc agcatgatcc gagggtggca tatgtcttct ccttcaagga
     2821 caccctggcc atatacaggc atgatgcaga gataaaacgg catctgcgct actcatcagg
     2881 tacaatttcc acttcgctgt gggaagctgc ccaggagaat aatctcaatc ccaacctctc
     2941 agctgagctg tcagagatct atgcatggac catagacttc ttcgggctgc agaagggtga
     3001 ccggttcaag gtgatttacg aggaggattt cattggtgac gagtcagttg gcatcaggcg
     3061 tgttcatgcc gcagaatttg agcatgccgg gagtacaatc tacgccattc cctatatcca
     3121 ggacagcgtc atgtcattct ttgacaccac cggagccagc ctgcgcaagg cttttctcaa
     3181 ggcgccgctg aggtactcgc gcatcagctc ccggttctcg ggcggcagga tgcatccgat
     3241 cctgaagatt gtaagggcac atcacggtgt tgactatgcg gccccactgg gtacccccgt
     3301 gcttgccata ggcgacggaa gggtgacgag cacagcctat gagaacgggt ctggaaggat
     3361 tgtacgcata acacacaaca gcgtctactc gaccgcttac atgcatctct cgcggttcgg
     3421 cccgggcatc tcatccggtg tatatgtcaa gcaggggcag gtgataggtt acgtggggag
     3481 ttccggcctc tccacagggc ctcatctcga tttcaggttc taccggaacg gatcacccat
     3541 tgatccgttg aaggttgagg caccgccggt caatcctgtt gccccggaag ccatggagga
     3601 gtttaacagg gtggctgagg ggtacattaa tctgctggca accataaaat attgaagatt
     3661 tgcgatttgc gatttgcgat ttgcgagtgt ggagataatt cgcaaatcga aaatcgaaaa
     3721 tcgcaaattc tctcgtcagc tttgctccac cagcttttct atgaccctgg gatagtgttc
     3781 atattccagt ttatgcaccc ttgcggcaag gctgtcagcg tcatcgccag gaagtaccgg
     3841 gcaccttgcc tggaaaatta tatcacctga atcatagtgc tcgttcacgt aatggatggt
     3901 gatgccgctc tcggtgcatc cggcatcgag caccgcacgg tgaaccctgt caccgtacat
     3961 tcccttcccc ccgaacccgg gcagaagagc aggatgaatg ttgacaatcc gtccacggta
     4021 cgcgccgatg acatcacccg gaaccagcca caggaaccct gccagaacca ccagatgtgt
     4081 ctccctcctg cgcagcatca ttagcacctc tccggtcgtg taaaattgag accggtcgaa
     4141 aaaaaatgca tccactccca atgccgctgc ccttttcaat accatagcct gaggcttatt
     4201 cgacaaaacg agggttactt ttgctttttt ttcgttcgaa aagtatctga tgatgttttc
     4261 agcgttggtc ccggcacctg aggctagtat tgctatattc ttcatcttct gatacttagt
     4321 ctgtttttca tcccgggtag ccggagtccc attttgagtc ttaaaatact gaatatttgt
     4381 gccttgcgcc tgcagagatg taaatttccg gcaaatttct tgaattatat ggtacaaata
     4441 tatttctttg cagagtttta aaaacgagta ttaacgtaaa attgattagt atgtctgaca
     4501 tttccacaag agtaaagaag atcatcgttg agaagcttgg cgttgatgaa gcagaggtta
     4561 ctcccgaagc acattttaca aatgaccttg gtgcggactc tcttgacaca gtcgagctga
     4621 tcatggaatt tgaaaaggag ttcaacatcg gaattcctga tgatcaggca gaaagcatca
     4681 gcacagtagg cgaagccatt aagtatattg aagagaacgc caagtagtta tctctgataa
     4741 cccataaatg aggcgtgtag tagttaccgg gatgggggcg ctgaccccca tcgggaacaa
     4801 tctgcaggat ttctggaaaa gtcttgtgga tggtgtcagt ggtgccgggc ttataaccca
     4861 ttttgacaca tccaagttca aaaccaggtt tgcctgcgag atcaagaact atgatgcttc
     4921 ccgttatttt gacaggaagg aatcgcgcaa aatggaccgt tacacccagt acgctcatgt
     4981 tgcggctgat gaagcggtaa aagatgcagg tcttgatccg gaagcaacag acaaagaccg
     5041 ttccggtgtg atctgggcct caggcatagg tggtatagag actattctgg aagaggtaaa
     5101 ggggtttgta atgggggatg gcaccccccg tttcagcccc ttcttcatcc ccaagatgat
     5161 cagtgacatt gccgccggga tgctctcaat caagtatggc ttccgcggac ccaatttcgc
     5221 cacagtttcg gcatgcgcct cttctacgaa tgcaataatt gacgcttact actacattct
     5281 tctcggcaag gctgatctgt tcattgccgg agggtctgag gcatccatca atccgcctgg
     5341 cattggcgga ttcaacgcca tgcaggccat gtcaaccaac aatgaagaat atgccacagc
     5401 ctcccgtcct tttgatgtga cccgcgacgg attcgtcatc ggcgagggcg ccggcgcact
     5461 catactggag gagcttgatc atgccagggc caggggcgca cggatttatg cagaggtgat
     5521 aggttccggc ctgacagctg acgcctatca tattactgct cctcaccctg acggcatggg
     5581 agctgtcaac gtgatgaaac aggctatcaa tgaatccggc ctgaagccct ctgacgttga
     5641 ctatatcaat gtgcacggca ctgccacacc gcttggtgac atcgctgaga taaaagccat
     5701 cctggctctc ttcggcgaac acgcctacaa gctgaacatc agtgcaacaa aatcgatgac
     5761 cggccaccta cttggcgccg ccggggttgt cgaagccata gcaacaacaa tggcggttgt
     5821 ccatgacatc attccgccaa caatcaactt caggaatcct gatc
//
LOCUS       DAOPXU010000142         5705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_17627_length_5705_cov_12.573097, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000142 DAOPXU010000000
VERSION     DAOPXU010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17627_length_5705_cov_12.573097"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PKK03_11135"
     CDS             <1..362
                     /locus_tag="PKK03_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX84994.1"
                     /translation="YIQADMELLNDFQFWGKSFLYLIPVAIVVQIVIQILFAIANRII
                     TQEDLPDITDERDKLIELKCIRISHWIFILGFMLAMGSLAAGMKPYVMFLTLISSGFL
                     ATLASEIAKIIYYRMGA"
     gene            364..639
                     /locus_tag="PKK03_11140"
     CDS             364..639
                     /locus_tag="PKK03_11140"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX84995.1"
                     /translation="MKEEKIMISNNIRRLRFFANEMTQQELADKTGVTRQTIVAIENG
                     KYFPSLELAFRIAYTFSVQLSDVFAYFPENDPVHKKVIAKQSKKEKQ"
     gene            641..1636
                     /locus_tag="PKK03_11145"
     CDS             641..1636
                     /locus_tag="PKK03_11145"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNX84996.1"
                     /translation="MKAIIYTEYGEPDVLHLQEVEKPSPKSNEVLVRVHAVSVNYGDI
                     IARKIRNLPAGEFNMLSLFRVMARFGFGYSKPKRQILGNTFAGEVETAGSTVKKFKAG
                     DQVFGYTGETMGAYAEYLCIPEDGIIDAKPSHLTYEEASVVPYGATMALCLLRKVNIQ
                     KDWKVLVIGASGAIGSAAVQLAAHHFGAEVTGVCSTQGIEYVKSLGAVRVIDYKKDDF
                     AKSGESYDLIIDILGKGSFSGYRGSLRQKGIYMPVSFKTGKLLQMLCTSVSGGKRVIC
                     ALATPKQEDLELIREFLEGRKLRPVIDQSFPLEKAAEAHRYVEAGNKKGSVVLTV"
     gene            complement(1734..2486)
                     /locus_tag="PKK03_11150"
     CDS             complement(1734..2486)
                     /locus_tag="PKK03_11150"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HNX84997.1"
                     /translation="MYTRAIVRRPGRNFASGITTSNLGRPDSGKALEQHGAYCEALIK
                     CGVALTVLDADERYPDGCFVEDTAVVNSRVKVISRPGATTRRGEEEAIAQVLAGYGPA
                     EAITAPGTLEGGDILRAENHYFIGISERTNAEGASQLAAILSRYGFTTSFISVEAGLH
                     LKSDIAYLGDGSFISTPVFSQVAQPANTIIPEPEEYYSANCLRVNDFLLVAKGFPKSR
                     KKILELGYNIIELDMSEFRKMDGGLTCLSLLF"
     gene            2633..3085
                     /locus_tag="PKK03_11155"
     CDS             2633..3085
                     /locus_tag="PKK03_11155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX84998.1"
                     /translation="MKTIDSRKPTTQEQLAAKESYESLVSSIGLLQNNLAEIEISETG
                     KRIRIPVSVLKLLVQILKEISQGNPVTVVPEATEITTQAASEVLGCSRPHVVRLLEEG
                     KIPYSKVGKHRRIRYDDLMSFKKKMKVSQKKKLQDLMKLDEESGLYDT"
     gene            3075..3638
                     /locus_tag="PKK03_11160"
     CDS             3075..3638
                     /locus_tag="PKK03_11160"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNX84999.1"
                     /translation="MIHSVRFTCVLDTNVFYPLWIRDLLLWFAYHDLYTPKWSNHIFN
                     EWIEAMLRKGITEIEAHRRTKIVNDAFPDALVENYEPLIESLSLPDLKDRHVLAAAIK
                     VNANLIVTNNLKHFPKEYLANFGIAATDADDFLTDIIDLNYRTSIKAFKDMVTSKIRP
                     PLDYYQVLEILRKNGLKNTADHLHSLL"
     gene            3828..5153
                     /locus_tag="PKK03_11165"
     CDS             3828..5153
                     /locus_tag="PKK03_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HNX85000.1"
                     /translation="MSPPRKRTRFKIGPGALVTAAFIGPGTITTCTLAGASFGYALLW
                     GMVFSVAATIILQEMAARLGIISHNGLGEALRTHFSKPASKLFTAILVISAITIGNAA
                     FQTGNLLGASLGLETLFGSGAPSASGSAAIAAGAAAAGSAAPVGSGSLFSSGILTLRF
                     WVAINAAIAFILLFVGSYKLLEKFLVALVILMSLTFVTTAIIVAPALPALLKGMFVPT
                     IPAGAVLTLVGLIGTTVVPYNLFLHASAVQERWHSPSDLPEARWDLSVAMILGGFISM
                     AVIVTAAAAFAGTGTNITSGADLAQQLEPLAGRWAKYITSIGLFAAGISSSITAPMAA
                     AYATAGIMGWKRDLRSARFRMVWMFILLAGVTFSMLGFKPVEAILFAQVANGILLPVI
                     AVYLLVVMNSRKIMGDNVNTLLSNILGGIVVLTATGLGIRSILHVIGIL"
     gene            complement(5256..5456)
                     /locus_tag="PKK03_11170"
     CDS             complement(5256..5456)
                     /locus_tag="PKK03_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX85001.1"
                     /translation="MKNSLKVERAIRNITQEELAREIGVSRQAINSIELGKFVPSTVL
                     ALRLSKYFDKPVNDLFSLEEGD"
     gene            complement(5446..>5705)
                     /locus_tag="PKK03_11175"
     CDS             complement(5446..>5705)
                     /locus_tag="PKK03_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85002.1"
                     /translation="FIISAFFVAFSREKTEDEFIARIRLESLLWATYINYGILLFCFM
                     FFYDFGFFYVMIINMFTVLVIFIIRFQYLLHRAKKSLSHEK"
BASE COUNT         1512 a         1463 c         1338 g         1392 t
ORIGIN      
        1 attacatcca ggcagatatg gagctgctga acgattttca gttctggggg aaatcgtttc
       61 tttaccttat accggtggca atagttgtac agatagtgat ccagatcctt tttgccattg
      121 caaaccgcat aatcacccag gaagatttac ccgacatcac cgacgagcgg gataagctga
      181 ttgaactgaa atgcatccgg atctcgcact ggatctttat actgggattt atgctggcga
      241 tgggatcgct ggccgcggga atgaaaccat atgtgatgtt tcttaccctt atctcatcag
      301 gattcctggc caccctggca tccgagattg caaagatcat ttattacaga atgggggcgt
      361 gatatgaaag aggaaaagat aatgatcagc aacaacatcc gcaggctacg attctttgcg
      421 aatgagatga cacagcagga gctggctgac aaaacagggg ttacacgcca gacaattgtg
      481 gcaatcgaga acggaaagta cttcccctcg ctggaactgg cattccgcat tgcttacacc
      541 ttcagcgttc agttatccga cgtttttgca tatttcccgg agaatgaccc ggtgcataaa
      601 aaagtaattg ccaaacagtc aaaaaaagaa aaacagtaaa atgaaagcaa taatatatac
      661 agagtatgga gaaccggatg tccttcatct gcaggaagtg gaaaagccaa gccccaaaag
      721 caacgaggta cttgtcaggg ttcatgctgt ctcggtaaac tacggggata ttatagcccg
      781 taaaatcagg aacctgccgg caggtgagtt caatatgctg tcattgttca gggtaatggc
      841 gcggtttggc tttggctaca gcaagcccaa aagacagatc ctgggaaaca catttgccgg
      901 ggaggttgag actgcgggca gcaccgtgaa aaaattcaaa gcaggcgacc aggttttcgg
      961 ctatacaggc gagaccatgg gcgcatatgc cgaatacctc tgtatcccgg aagacggcat
     1021 cattgatgcg aaaccttcgc atctgacata tgaggaggct tcggtagtcc cttacggagc
     1081 gacaatggcg ctctgtttac tgaggaaggt gaatattcag aaagactgga aggtgctggt
     1141 tatcggtgcc tccggggcga tcggttcggc agccgtacag ctggccgcac atcatttcgg
     1201 ggctgaggtc acgggtgttt gcagcactca gggaattgaa tacgtaaaga gcctgggagc
     1261 agtcagggtc attgattata aaaaagacga ctttgcgaaa agcggggagt catatgacct
     1321 catcatcgac atcctgggga aaggatcctt ttcaggatac aggggatcac tgaggcaaaa
     1381 agggatttac atgccggtaa gcttcaagac cgggaaactg cttcagatgc tatgcacgtc
     1441 tgttagcgga gggaaaaggg tcatttgcgc gcttgcaact ccaaaacagg aggaccttga
     1501 attgataagg gaattccttg aaggccggaa gctcaggcct gtaattgatc agagtttccc
     1561 ccttgaaaag gcagccgaag cccacaggta tgttgaagcc gggaacaaaa agggcagtgt
     1621 ggttctgaca gtttagtaca attgccggta caggaaattt aaccttataa attcgcaaat
     1681 ccgccagccg gcggacacag tcgcaaatcg caaatcgcaa ttcccgggtc ctgtcagaac
     1741 aacagcgaga gacaggtaag gccgccgtcc atcttcctga attctgacat atcaagctcg
     1801 ataatgttgt agccgagctc aagtatcttc ttccttgatt ttgggaatcc tttcgcaaca
     1861 agaagaaaat cattgacccg cagacaattg gccgaataat attcttcagg ttcagggatg
     1921 attgtatttg ccggttgagc gacctgcgaa aacaccgggg tggaaatgaa gcttccgtct
     1981 ccaaggtatg cgatgtccga tttcaggtgc agtcctgcct ccacactaat aaatgaagta
     2041 gtgaaaccat atcttgaaag gattgctgcc agctggctgg ctccttctgc attcgtcctt
     2101 tcggaaatgc cgatgaagta atggttctca gcccggagaa tgtctccccc ctccagggtg
     2161 ccgggcgctg tgattgcttc cgctggtcca tagccggcca gcacctgtgc gatggcctcc
     2221 tcctctcccc ttcttgttgt ggctcccggc cgtgatatga ccttcaccct gctgttgaca
     2281 acagccgtat cctccacgaa acagccgtct ggatatcttt catcggcatc cagtactgtc
     2341 agtgccacac cgcattttat aagcgcctca cagtatgcac catgctgttc gagggctttg
     2401 ccggaatccg gccgcccgag gtttgatgtt gttatcccgc tggcaaagtt cctgcccggt
     2461 cttcttacta ttgctcttgt atacaattct ttatttgatt tttgaatgtc tctgttacct
     2521 caagtataat caaatttcgg gtgaactcaa actcatagca tttttattca tttattttgt
     2581 ttgtttcgtt tatttcgttt aattttgcat aaagtcaaac tatgatcaca caatgaaaac
     2641 aatcgatagt cggaaaccaa ccacacaaga gcagctcgcc gcgaaagagt cctacgaatc
     2701 gcttgtctca tctataggat tgttgcagaa caatttagct gagattgaga tctcggaaac
     2761 aggcaaaaga atcaggattc ctgtcagtgt gttgaaactg ttggtccaaa ttcttaaaga
     2821 gataagccag ggtaatccgg ttaccgtagt cccggaagca actgaaataa caacgcaggc
     2881 agcctcagaa gtcctgggtt gttcaaggcc tcacgtcgtt cgcctgcttg aggaaggtaa
     2941 aattccctac tcgaaggttg gcaaacacag gagaatcaga tacgatgacc taatgtcatt
     3001 caaaaagaaa atgaaggtat cccagaaaaa gaaattacag gatcttatga aacttgatga
     3061 agaatcagga ttgtatgata catagtgttc gatttacctg cgttcttgac accaacgtct
     3121 tctatcctct ttggatacgg gacttattgt tatggtttgc ataccatgat ttgtacactc
     3181 ctaaatggag taatcacatc tttaatgaat ggatcgaagc aatgctccgg aaagggatca
     3241 cggaaattga agcacacagg aggaccaaaa tagttaacga tgcttttccg gatgcattgg
     3301 ttgaaaacta tgaacctttg atagaatctc tttcacttcc tgacctcaag gacagacatg
     3361 tgcttgcagc cgcaatcaag gtaaatgcta acctgattgt cacaaataac ctgaaacact
     3421 ttcctaagga gtaccttgct aattttggta ttgcagcaac ggatgcggat gactttctga
     3481 cagacattat cgacctcaat tatagaacga gtatcaaggc cttcaaagat atggttacaa
     3541 gtaaaatcag acctcccctt gattattacc aggtacttga aatcctcagg aaaaacggat
     3601 taaagaatac ggcagaccat cttcattccc tgctataata atcactcgtt atggattgta
     3661 ttcccaaaat attataaaat tcaaggtata taatccatat attctgatat cgaatatacc
     3721 atacccgatc caaacccatg ccaactgcag aatggagact gctgtctccc tgatatggtc
     3781 ataattcaat ttttcttatt tttataatac ccatatttaa tcacatgatg tcccctcccc
     3841 gaaagcgcac cagattcaaa atcggccccg gagccctggt cacagcagcg ttcatcggac
     3901 cggggacaat cacaacctgc accctcgccg gcgccagctt cggttacgct ctcctctggg
     3961 gaatggtctt ctcggtggcg gcaacaataa tcctccagga gatggcggca cgactcggca
     4021 tcatctcaca caacggcctt ggcgaggctc tccgcacaca cttcagcaaa ccagcctcca
     4081 agctgttcac agctatcctc gtcatctcgg caatcacaat aggcaatgcc gccttccaga
     4141 ccggcaacct gctcggcgca tcactgggcc tcgagacact tttcggatcc ggggcccctt
     4201 cagcctcagg gtcagcagcg atagcagccg gggcagcagc agccggatca gcagcaccgg
     4261 tcggctctgg ctccctcttc agttccggca tcctgaccct ccgtttctgg gtggccatca
     4321 acgccgccat tgccttcatc ctcctcttcg tgggcagcta taagctcctg gaaaaattcc
     4381 tggtggcact ggtgatcctt atgagcctca ccttcgtcac aacagcaata attgtggccc
     4441 ctgccctgcc ggcgctgctg aaggggatgt tcgtcccgac aatacctgcc ggcgcagtat
     4501 taacccttgt cggactcatc ggcaccacgg tggtccccta taacctcttc ctccatgcct
     4561 cggcagtaca ggaaaggtgg cacagtccgt ccgacctccc ggaggcaaga tgggatctct
     4621 cggtggcgat gatcctagga gggtttatat ctatggccgt catcgtcacc gcagcagcag
     4681 cctttgcagg caccggtacg aatatcacct ccggcgccga cctcgcacaa cagcttgaac
     4741 cccttgccgg ccgatgggca aaatacatca ccagcatcgg cctcttcgcc gcaggcatct
     4801 cctcctcaat caccgccccc atggccgccg cctacgccac cgccggcatc atgggctgga
     4861 aacgcgacct ccgctctgca cgcttccgga tggtgtggat gttcatcctc ctggctggcg
     4921 tcaccttctc aatgctcggg ttcaaaccgg tcgaggccat tctcttcgcg caggtcgcca
     4981 acggcatcct gctgccggtg atagccgtct acctcctcgt ggtgatgaac agccggaaaa
     5041 taatgggtga caacgtcaac acactcctct caaacatcct cgggggtatc gtcgtgctga
     5101 cagcgactgg actgggcatc cggagcatac tgcatgtaat cggcatactc tgacagcctg
     5161 cggccgcgca tctgataaag gcatccggca gaggtctgca acctcaggca accgatggaa
     5221 ttgcaggaga atcagctctg ccctaccccg gagctctaat caccctcttc aagactgaac
     5281 aggtcgttta ccggtttgtc aaaatacttt gagagcctga gagccaggac ggtcgacggc
     5341 acgaacttgc caagctcaat cgagtttatt gcctgcctgc tcacccctat ctccctggcc
     5401 agctcctcct gggtgatatt cctgattgcc ctctcaacct tcagactatt tttcatgact
     5461 cagcgatttt ttggctctgt gaagtaaata ctgaaaccgg attatgaata tcaccaggac
     5521 tgtaaacatg ttgataatca tcacatagaa aaatccgaaa tcataaaaga acataaaaca
     5581 gaacagcagg atgccataat tgatgtatgt agcccacaac aatgactcaa gcctgattct
     5641 ggcaataaac tcatcttctg tcttttccct cgagaatgca acaaagaaag cgctgataat
     5701 gaaaa
//
LOCUS       DAOPXU010000143         5672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_17782_length_5672_cov_14.133701, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000143 DAOPXU010000000
VERSION     DAOPXU010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17782_length_5672_cov_14.133701"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PKK03_11180"
     CDS             <1..841
                     /locus_tag="PKK03_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX85003.1"
                     /translation="IPPDFSEGLAGVTRPRLQMLVDAVNATSAQLSWSYLTSVVRDFN
                     RDVIVSTGMMPGNTAALSGGSVPGISVSPRYWYNPTLNYKYYMLPGILVILITAIGLM
                     MSGLNLVREKESGTIEQINVTPVMKWQLIASKIIPFFIIGLLDLALGLSIGWLAFNIP
                     FEGSLLLFFACAAIFLVAVLGLALFFSTMASTQQQFLFVAFFFVMIFILMSGIFTPTE
                     SMPLWAQQFNWVNPVAYLMRINRMVMLKGSGFSDIMRDMGMLTVLAISFLTLAIRAYR
                     KRA"
     gene            complement(886..1281)
                     /locus_tag="PKK03_11185"
                     /pseudo
     CDS             complement(886..1281)
                     /locus_tag="PKK03_11185"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1278..1655)
                     /locus_tag="PKK03_11190"
     CDS             complement(1278..1655)
                     /locus_tag="PKK03_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNX85004.1"
                     /translation="MNTKAPSNIDDYISLQAPEVQPLLKKVRETILDAAPGAEEYISY
                     MMPSFRYNGKILVYFAAHKNHLGFYATPSGNIAFSEELKDYKSSKGAVQFPLDRPIPY
                     DLIRRMVQYKLEEIMASSTKKRI"
     gene            complement(1753..2598)
                     /locus_tag="PKK03_11195"
     CDS             complement(1753..2598)
                     /locus_tag="PKK03_11195"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNX85005.1"
                     /translation="MLRSLITAALFAILFFSCVPADDPAIDVMTLNIRMDTPRDSINA
                     WPNRAAIVAGFVNDQIPDLLGMQEVLWHQYKYLDSALIGYGSVAAGRDDGLRAGEACP
                     VFWRLGRFERLDTGTFWLSATPDVPGSIGWGAALTRVATWVKLYDKVKQDTLLFFNTH
                     FDHISDSARVMSSGVLLGMVRKLAGDNEFVITGDFNALPESLAISRMKEGDFAVDSYL
                     ISETPPAGQSYTFNGWKDEPGEGRIDYIFIRNGMKTLSHETHRVIEDGVFISDHWPVT
                     ARIRQ"
     gene            2650..3801
                     /locus_tag="PKK03_11200"
     CDS             2650..3801
                     /locus_tag="PKK03_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HNX85006.1"
                     /translation="MSKKRTIAEINEKILSGKAVVLTAEQVAAMGREATPAEIAEKVD
                     VVTTGTFGPMCSSGMFINFGHTDPPMRMERITLNNVPVFAGIAAVDAYIGATETALPH
                     DQYGGAHVIEELIAGRDVMMQATAKGTDCYPRTEVKTLINRESVNEMIMFNPRNAYQN
                     YNVAVNSTSKMKYTYMGTLLPSFGNATFSTSGELSPLLNDPEMRTIGIGTRIFFGGTT
                     GYVAWHGTQFNTGKPVNEHGVPVGNSATLALIGNAKEMNTRFIRAAYFEKYGVSIFVG
                     VGIPIPVLDEDLARRVMIRNSQIETNVLDYGSPGHDLLGRANYESLFSGEISLNGKKI
                     RTAPLSSLRVAREIAETLRGEIAAGRFLLTAPVENFKMTSGLNNLEIRL"
     gene            3823..4233
                     /locus_tag="PKK03_11205"
     CDS             3823..4233
                     /locus_tag="PKK03_11205"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HNX85007.1"
                     /translation="MKSRRFVLTFPPEATGEPITYNLIRKFDIMVNIVKADVSPGQVG
                     HLVMEMSAPSKVLKDGIEYMKQQNVECVPIDRTITYRKELCVHCGACSAVCFAGALTM
                     DRKSAELVFEPEKCVVCELCLTACPLKLFEIDLG"
     gene            4226..4984
                     /locus_tag="PKK03_11210"
     CDS             4226..4984
                     /locus_tag="PKK03_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HNX85008.1"
                     /translation="MAKSTAYQPRLYREAMGNSRWTSFRCAFRETDLWVAIDSSHYHK
                     EVERFTMDRIIFYRRVLEEHIKLHPEFRDSLTPVVAPGGVHPLITDMSDAALAAGTGP
                     MSAVAGAMAEYICRDMLAETGADEVIIENGGDIFMKLTAPATVAIFAGDSPLSEKIAL
                     QPVPGDTPLSLCCSSGTVGHSLSFGVADACMIACRSGALADACATAFCNMVKNPEMVR
                     EVTEQALKMREILSVVIIAGDKVGLGGTIAVRFV"
     gene            complement(5000..>5672)
                     /locus_tag="PKK03_11215"
     CDS             complement(5000..>5672)
                     /locus_tag="PKK03_11215"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNX85009.1"
                     /translation="NTEVQIYRGYWMISWFKKEFGLKEIQMAEQTGRHPETFFDAMVD
                     DVPAGSMGLVLQPYWSPGVKVPGTEAKGAIIGFGDVHTRAHVYRAILEGLTYSLKEGL
                     NRTVKRTKIPARSVVVSGGGSQSRQAMQITADIFNLETFRAHTYETSALGAAINAAVG
                     MKYYSDFPAAVGAMTRRGDSFTPDPHNVEIYSELYEKVYSKMYRHLQPLYEQIRDVTG
                     YPPKI"
BASE COUNT         1316 a         1515 c         1464 g         1377 t
ORIGIN      
        1 cataccgcct gatttttccg agggacttgc cggagtcacc cgcccgcgcc tgcagatgct
       61 tgtcgatgcc gtaaacgcca ccagcgcaca actctcatgg agctatctca cgtcggtagt
      121 acgcgatttc aaccgggatg tgattgtcag caccgggatg atgcccggta acacagcagc
      181 cctctcagga gggtctgtgc cggggatctc cgtttctcca cggtactggt acaacccgac
      241 attgaattac aagtactata tgctgccggg catactggta attctcatca cagcgatcgg
      301 actgatgatg tcaggactta acctcgtcag ggagaaggag agcggaacca tagagcagat
      361 caacgtcacc ccggtaatga aatggcagct gatagcatcc aagatcatcc ccttcttcat
      421 catcggtctc ctcgatcttg ccctggggct gagtatcggg tggctggcat tcaatatccc
      481 cttcgagggg agtcttctcc tcttcttcgc ctgtgcggcg atattcctcg tggcagtgct
      541 tggactggcg cttttcttct ccaccatggc atcgacgcaa cagcagttcc tctttgtcgc
      601 attcttcttt gttatgattt tcatcctgat gagtggtatc tttactccca ccgagagcat
      661 gccgctatgg gcacagcagt tcaactgggt taatccggtg gcttacctga tgcgcataaa
      721 ccgtatggtg atgctgaagg gatcaggttt cagtgatatc atgcgggaca tggggatgtt
      781 gaccgttctt gcaatctcat tcctcacact ggcaataagg gcataccgca agagggcgta
      841 acccttatct gaccggttct ttcctgccct gccgggcgaa atgaatcagc cggccgcctc
      901 agtcagtttc cggatgatat gttcctcctg gccgctgtac agctgccacg atactccgtg
      961 cctgtcttcg acccgtccgt atttcggact gaaaggatac tcatccagcg gcatcagcgt
     1021 ctttccgtct tcaatcagcc ttgagtagat ttcctccact tcgctctgcg atgaggtgag
     1081 gtacatcagg gagatttacg gatttggccg gtacatgtcg ccgccgttta gaagcatcag
     1141 tttctgacca cttatttcca ttattaccac caccgggttt tcgtctgcaa ttttcgattt
     1201 cgggaaggcg gatatgtaaa attccgcgat ttccctggcg ttgttgttgc accagaccga
     1261 tggatagatt gagttgttca tattcttttc tttgttgatg acgccatgat ttcttcaagt
     1321 ttgtactgaa ccatcctgcg gatcaggtcg taagggatag gtctgtccag cggaaactga
     1381 accgcccctt tggatgactt gtagtctttc agttcctcac tgaatgcaat attcccggag
     1441 ggtgtggcat agaagccgag gtgattttta tgcgcagcaa aatagaccag aattttgccg
     1501 ttgtatctga acgagggcat catgtagctg atgtactctt cagcacccgg ggctgcatca
     1561 agaatggttt ctctcacctt cttcagcagt ggctgcactt caggcgcctg cagcgaaata
     1621 taatcgtcga tgttcgaggg agcttttgtg ttcatgatcc gcgtgaatta tatataaaac
     1681 aattcctgac ggattttgat catttgttgc cgtatttgac caagtcaggc acaactgcct
     1741 gttgcctgat gcctattgcc ttatcctggc ggtcaccggc caatggtccg atatgaacac
     1801 accatcctca atcaccctgt gcgtctcatg cgacagtgtc ttcatgccgt ttcggatgaa
     1861 aatataatct atcctccctt cacccggttc atccttccag ccgttgaagg tatatgactg
     1921 acctgcaggc ggggtttccg atataagata tgaatcaact gcaaagtcgc cttctttcat
     1981 gcgcgaaatc gccaggcttt ccggcagcgc gttgaaatca ccggtgatca caaattcatt
     2041 gtcacccgcc agttttctga ccattcccag cagcacgccg gaactcatca ccctggctga
     2101 atcgcttata tggtcgaaat gagtattaaa gaacagaaga gtatcctgtt tcactttgtc
     2161 atagagcttc acccaggtcg ccacccgtgt cagtgccgcg ccccatccta ttgatccggg
     2221 cacatccggg gtggcagaca gccagaaagt tcccgtgtca agcctttcaa atctcccgag
     2281 ccgccagaaa acaggacaag cctcgcctgc acgcagaccg tcatcacgtc ctgctgccac
     2341 cgaaccatat ccgatcagtg ctgaatcgag gtacttatac tggtgccaga gaacctcctg
     2401 cataccaagc agatcaggta tctggtcgtt gacaaaacct gcaacaatag cagcccggtt
     2461 aggccatgca ttgatactgt cgcgcggagt atccatcctg atgttgagtg tcattacgtc
     2521 aattgcaggg tcgtcagccg gtacacatga aaagaaaaga attgcaaaaa gagcggcggt
     2581 gataagtgat ctaagcatat ctttgaagtt tgtttgaaag caggctaaag ttactttaaa
     2641 atttacatta tgtcgaagaa acgaaccatt gcggagatca atgaaaagat cctcagcgga
     2701 aaggcggtag tgctgacggc cgaacaggtt gcagccatgg gtcgcgaagc aacgcccgct
     2761 gagattgcag aaaaggttga cgttgtcacc acaggcacct tcgggccgat gtgctcgtcg
     2821 ggcatgttta tcaacttcgg tcacaccgat ccgcctatgc gcatggagag gatcaccctg
     2881 aacaatgtgc ctgtctttgc cgggatagct gccgttgatg cctacatcgg ggccaccgag
     2941 acagcactgc cgcacgatca gtacggcggt gcacatgtca tcgaggagct gatagcaggg
     3001 agggatgtga tgatgcaggc cactgcgaag ggcaccgact gctacccgcg caccgaggtt
     3061 aaaaccctta tcaaccgcga gagcgtcaat gagatgataa tgttcaaccc gcgcaatgca
     3121 taccagaact ataatgtagc ggtgaacagt acctcaaaaa tgaaatatac gtatatggga
     3181 actctgctcc catcgttcgg caatgccacc ttttcaacct cgggtgagct cagcccgttg
     3241 ctgaatgatc ccgaaatgcg gaccatcgga atcggtacac gcatcttttt cggcggtacc
     3301 accggttatg tggcatggca cggtacgcag ttcaacaccg gaaagccggt caacgaacac
     3361 ggcgttcccg ttggcaactc agccactctg gcacttatcg gcaacgcgaa ggagatgaac
     3421 acccgattca tcagggcggc atactttgag aaatacggag tgtcaatctt tgttggggtc
     3481 ggtataccca tcccggtgct tgacgaagac cttgcgcgca gggttatgat ccgcaacagc
     3541 cagatagaaa caaacgtcct tgactatggc tcacccggtc atgacttgct gggacgcgcc
     3601 aactatgagt ctctcttctc cggagagatt tccctcaacg ggaagaagat acgtactgcc
     3661 ccgctctcga gcctgagagt ggcacgcgag atagcagaaa cactccgcgg ggaaatagca
     3721 gcaggacggt tcctgctgac agccccggtg gaaaacttca agatgacctc cggcctcaat
     3781 aacctggaga tcagacttta acatttaatt tttaatgaca ttatgaaaag caggagattt
     3841 gtgctgactt tcccgcccga ggcgacgggt gagcccataa catataacct tatcaggaaa
     3901 tttgacatca tggtcaacat agtaaaagct gacgtttcac ctggccaggt gggccacctt
     3961 gtgatggaga tgtctgcccc gtcaaaagtc ctgaaggacg gaattgaata catgaagcag
     4021 cagaatgtcg agtgtgttcc cattgaccgt acgataacat accggaagga actgtgtgtg
     4081 cactgcggtg cgtgttcggc ggtttgtttt gcaggagctc tgacaatgga ccgtaaatca
     4141 gctgagctgg tgttcgaacc tgagaaatgc gtggtctgtg agctctgtct cacagcctgc
     4201 ccgctgaaac tatttgaaat cgatcttggc taaaagcaca gcatatcagc cccggctgta
     4261 ccgcgaagcc atgggcaaca gccggtggac gagcttccgt tgcgctttcc gcgaaacaga
     4321 cctctgggtg gcaattgaca gcagccacta ccacaaggaa gttgaacggt tcaccatgga
     4381 ccggatcatc ttttaccgca gggtgctgga ggaacacata aaattgcacc cggagttcag
     4441 ggattcgctg actccggttg tggcacccgg cggggttcac ccgctgatca cggatatgag
     4501 cgacgctgca cttgccgccg gcaccgggcc gatgagcgcc gtggccgggg caatggctga
     4561 atatatctgc agggacatgc tggcggaaac aggggctgat gaagtgataa ttgaaaatgg
     4621 cggcgacatc ttcatgaaac tgacagcccc ggccacggtg gcgatatttg caggagactc
     4681 cccgctgtca gaaaagattg cccttcagcc ggtacctggc gacacgcctc tgtcactctg
     4741 ctgttcgtcc ggaacggtag gtcattcgct tagcttcggg gttgctgatg catgcatgat
     4801 tgcatgccgt agcggcgctc ttgccgacgc ctgtgccact gccttctgca acatggtgaa
     4861 aaacccggaa atggttcgtg aagtgactga acaggcgctg aaaatgagag agatcctgtc
     4921 agttgtcata atcgcaggtg ataaggtcgg cctgggtggc accattgccg tcaggttcgt
     4981 ctgacggcac ctggcagcgt cagatcttcg gcgggtagcc cgtcacatcc ctgatctgct
     5041 catacagcgg ctgcagatgg cggtacatct tcgagtaaac cttttcatac agctcactgt
     5101 aaatctccac attgtgcggg tcaggcgtga aactgtcacc tctcctggtc attgccccca
     5161 ccgctgccgg gaagtcgctg taatacttca tccctacagc cgcgttaatt gccgccccga
     5221 gagccgatgt ctcataggtg tgagccctga aggtctcaag gttgaatata tcagcagtga
     5281 tctgcatggc ctgcctgctc tgcgaacctc ctccggaaac cactacgctt cgtgcaggga
     5341 ttttcgtacg tttaaccgta cggttaagtc cttccttaag tgaataggtg aggccttcaa
     5401 gtatggcgcg gtatacatgg gcacgggtat gaacgtcacc aaatcctatg attgcaccct
     5461 tagcttcggt tccgggtacc ttcacgccgg gcgaccagta tggctgtagc accaggccca
     5521 ttgagcctgc cggcacatcg tcaaccatgg catcaaagaa ggtctcaggg tgtctgccag
     5581 tctgctcggc catctgtatc tccttcaggc cgaactcctt cttgaaccag ctgatcatcc
     5641 agtaacccct gtagatctgc acctcggtgt tg
//
LOCUS       DAOPXU010000144         5655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_17863_length_5655_cov_11.144821, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000144 DAOPXU010000000
VERSION     DAOPXU010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17863_length_5655_cov_11.144821"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PKK03_11220"
     CDS             <1..302
                     /locus_tag="PKK03_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85010.1"
                     /translation="SNFDWDNDGNSMVVSVERKGQIIPLSGEDEWATSKIQIYEGEIG
                     KSLDFRFYKRKGELVKNNNVVVSVQNQYGENLPFFSAPIGGVPVYKPRIKVVPKK"
     gene            359..3463
                     /locus_tag="PKK03_11225"
     CDS             359..3463
                     /locus_tag="PKK03_11225"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNX85011.1"
                     /translation="MLHPFVSSAQTYSFRTYDEYTGLPGPWLSVIAQDRSGLLWMGVD
                     TGLYRYDGFSFHHMPFPDTLPRGNVSALFCDRKGTMWVGMNDGSLFTWNMTGGLERMT
                     LEESDRINRITGSPDGKVWIVTQTSGIFIAGSGGDEKITRMKMPDDMTLFDIAFAGEG
                     TFLAATQNNLHLCDAEGDKAVSRLTFPELDYTWIQTVVRMDDDTWFAGTDNTGLWMIR
                     RSGDSINARAVGDTLFMNMNIPALNAGPERTLTIATKDSGAVKVAFSTDYSKMIITGR
                     YDVRSGLPDNNVRTIFTDREENLWMGLYTSGLAAVTTNAFAFYRPETDKEITYIGRAG
                     TQVVAGTRSGLFDFDPLTGSFTRFRNLSARTGGSGITSWSADDPGNIWIGTAGDGLWL
                     MKPDGSLKQVYRSGNPGQNHINSVAVEGDNTWLGTSDGVVLLDRETGRQKAVYTTMEL
                     LPHNYISQVVVNRDGEVLVATKSDRLCYIHVTGGVRTENEVMEGRLVNDILSISVAPD
                     RSVCAGTKGNGLFLFAADTLLNYTEDDGLLNNFCYSAMIASDGRIWTGHQKGFSITNP
                     ATGSVRSYSADFGVRGDCLPNALTQLPDGTVCIGTTEGIVVYNPSLEPDSAVAPQAGI
                     ISVVINNVVYPYRESYNLPYRKSYTVKVNYAGIFLRDPLNVVYRTRLGRDEQWSEMTG
                     DRSVTYNSLRDGHYRFSVEALTRDDPGSATSASFDLIIQKPVTDRWWFFLSMLALIAG
                     VVYIIVRLRERAHRKQREYLESELQKRTAEVYEQKEELAQKNQDITESIKYAKRIQSS
                     VLPDTARLNNVFRDAFVFFQPRDIVSGDFYWFDWVDKDRFIVVCSDSTGHGVPGAFMS
                     MIGTALLQDIITVKKITRPSQILHELDRQIFSTLNQNQELEASNDGMDIVVCEFNMTT
                     RQLVFSSAMRPVILVIDGVQHYVRGNRSSVGGESVTEKFYDDQEYHLREGDLVFLFTD
                     GYCDQFGGTGGKKMKISRLKTLIDDITPLSAVDQQREIKDFFFEWMGKGEQVDDVLFM
                     GMRV"
     gene            complement(3464..3985)
                     /locus_tag="PKK03_11230"
     CDS             complement(3464..3985)
                     /locus_tag="PKK03_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85012.1"
                     /translation="MKNAIIITVIMGGLMMASCGPIETLPPEPSIEFRAFSLYDTTDM
                     LGNPAKAGKITFYFEDGDGDLGLDPPVIPGVDPSSNLFFRLYRKTNGTFELAAPSDPL
                     YPSEYRIPYIDAEGQNKITKGTIDVTLIYLIYNPDDTLYYDFWIKDRSGNESNTATTC
                     IVVLGENGTCLPE"
     gene            complement(4030..5334)
                     /gene="der"
                     /locus_tag="PKK03_11235"
     CDS             complement(4030..5334)
                     /gene="der"
                     /locus_tag="PKK03_11235"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNX85013.1"
                     /translation="MSKIAAIVGRPNVGKSTLFNRLTESRKAIVNEESGVTRDRHYGR
                     AIWNGVEFSVVDTGGFVEDSDDVFESEINRQVKLAISEADVILFMVDVETGIHELDKA
                     VAAMLRKVQKKVITVVNKVDNNKRLLDAAEFYGLGLGDYFPVSSVNGSGTGELLDEIV
                     KHLPDTPEEAGEDIPKVAVVGRPNVGKSSLANTLLGEERNIVTPVAGTTRDSIHSLYN
                     KFNHRFLLIDTAGLRKKAKVDEDIEFYSILRSVRAIEESDLCLLLIDATRGLEAQDMS
                     ILGIIQKNNKGVIILVNKWDLIGKDQTTAKQFEEEIRRKTAPFTDYPVLFISAITKQR
                     IHKVLELIMSVYENRSRKITTSKLNEVMLNIIGETPPPSTKGKHIRIKYVTQLPAEYP
                     AFAFFCNLPQYIRDPYKRFVENRLRENFQLTGAPVRIYFRKK"
     gene            complement(5347..>5655)
                     /locus_tag="PKK03_11240"
     CDS             complement(5347..>5655)
                     /locus_tag="PKK03_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNX85014.1"
                     /translation="EIIRGKIFETYQKEIPYSVEVEIESYREEPEINRIAAVIHVARD
                     SQKGIIIGHKGSMLKRVGTASRKEMEEFLGKKVFLELYVRVAHEWRDNPRMLKKFGYL
                     "
BASE COUNT         1358 a         1408 c         1494 g         1395 t
ORIGIN      
        1 agagtaactt cgactgggac aatgacggga attccatggt ggtatccgtt gagaggaagg
       61 ggcagataat cccgctttca ggcgaagatg aatgggcgac atcgaaaata caaatctacg
      121 agggagagat aggaaagtcg ctcgacttcc gcttttacaa gaggaaggga gaactggtca
      181 aaaacaacaa tgtggtggta tcagtacaga accagtacgg cgagaatctc ccgttcttca
      241 gcgcaccgat cggaggagtt cccgtatata agccacggat aaaggtagta cccaagaagt
      301 aatcatgccc ggaagattca gatattcaat tgcagcaata tgtattgttt gtctaaatgt
      361 gctgcatccg tttgtttcgt ctgcacagac gtacagtttt cgtacctatg atgagtatac
      421 cggccttccg ggaccctggc tgagtgtgat agcgcaggac aggagcggcc ttctctggat
      481 gggagttgac accggccttt accgttatga cggtttctca tttcatcata tgccttttcc
      541 tgacaccctg ccgcggggta acgtgagcgc cctgttctgt gaccggaagg gaacaatgtg
      601 ggtgggaatg aatgatggct cgctcttcac ctggaacatg acaggaggat tggagagaat
      661 gacgctggag gaatctgaca gaatcaaccg gatcaccggc agccctgacg gcaaggtttg
      721 gatagtcacg cagaccagcg ggattttcat tgccggttcc ggaggtgatg agaagatcac
      781 caggatgaag atgcccgatg atatgactct gtttgacatc gcctttgccg gagaaggaac
      841 attccttgca gcaacgcaga ataatctcca tctctgtgat gctgaaggcg ataaggcagt
      901 cagcaggctc accttccctg aactggacta tacatggatt cagacagttg tccggatgga
      961 tgatgacacc tggttcgccg gtaccgacaa cacgggcctg tggatgatcc gtcggagcgg
     1021 tgactcaata aatgccaggg ctgtgggtga caccctcttc atgaacatga atatcccggc
     1081 acttaatgcg gggccggaaa ggacactgac cattgccaca aaggattcgg gggcagtaaa
     1141 ggttgcgttc agcactgact atagcaagat gatcatcacc gggagatatg atgtcaggtc
     1201 aggtctccct gacaataacg tccgtaccat ttttaccgac cgggaagaga acctttggat
     1261 gggtctctat accagtggcc tggctgctgt cacaactaac gccttcgcct tttacaggcc
     1321 ggagactgac aaggaaatta catacatcgg ccgggccggc acacaggtgg ttgcaggcac
     1381 caggagcggg ctttttgact tcgatccgct taccggcagc tttacacggt tccgtaatct
     1441 ttctgcaaga acaggcgggt caggcatcac ctcatggtct gctgatgacc ccggaaacat
     1501 atggataggc actgcgggag acggtttatg gctgatgaag cccgacggga gcctgaaaca
     1561 ggtctatcgt tccggcaatc cgggccagaa ccacatcaat tcagtggccg tggagggtga
     1621 caatacctgg ctcggaacca gcgacggggt ggttctgctt gacagggaga caggcaggca
     1681 gaaagctgta tatacgacga tggaattgct gccgcacaac tatatctcac aggttgttgt
     1741 caaccgcgat ggagaggtgc tggtagccac aaagagcgac aggctgtgct acatacatgt
     1801 gaccggcggg gtacgtacgg agaacgaggt catggagggc aggctggtaa acgatattct
     1861 cagcatctct gttgctcctg accgttccgt atgtgcagga acaaagggca acgggctctt
     1921 tctctttgct gctgacacac ttctgaatta taccgaggat gacgggttgc tgaacaattt
     1981 ctgctacagc gcaatgattg cctctgacgg aaggatatgg accggccacc agaaaggctt
     2041 ttcaatcacc aatccggcaa ccggctcggt aaggtcctac agcgcggact tcggggtgcg
     2101 tggagattgt ctgcccaatg cgcttaccca actccctgac ggaaccgtat gtatcggaac
     2161 aacagaaggg atagttgtat acaatccttc actcgagcct gactcggcag tagcccctca
     2221 ggcagggatc atttctgtgg ttatcaacaa tgttgtttat ccctacaggg agagttacaa
     2281 cctcccctac aggaagtcct acactgtgaa ggtaaattat gccggtattt ttcttcgtga
     2341 tccgcttaac gtagtctacc gcaccaggct tggcagggac gaacagtgga gtgaaatgac
     2401 cggtgacagg tctgtgacct acaacagtct gcgtgacggg cactaccggt tcagtgtgga
     2461 agccctgacc agggatgacc ccggcagcgc aaccagtgca tcattcgatc tgataataca
     2521 gaaacctgtt acagacagat ggtggttctt cctctcgatg ctggctctga ttgccggtgt
     2581 ggtttacatc attgtgagac ttcgtgagag ggcccacagg aaacagcgcg aatatctcga
     2641 gagtgagctg cagaagcgta cggccgaagt ttacgaacag aaagaggaac ttgcacagaa
     2701 aaaccaggac atcactgaaa gcatcaagta tgccaagcgt atccagagca gcgtgctccc
     2761 ggacaccgcg cgcctcaaca atgtcttcag ggatgcattc gtcttcttcc agccgcgtga
     2821 tatcgtcagt ggcgacttct actggtttga ctgggtagac aaggaccgtt tcattgttgt
     2881 ctgctctgac tccaccggac acggggtgcc gggcgcattc atgtcaatga tcggcacggc
     2941 gctgcttcag gatataataa ctgtcaaaaa gataaccaga ccctcacaga ttctgcatga
     3001 gcttgacagg cagatattct ccaccctgaa ccagaaccag gagctggaag catcaaatga
     3061 cggcatggac attgttgtat gcgaattcaa catgaccacc aggcagttgg tattctcatc
     3121 agcgatgaga ccggtaatac tggtcattga cggggtccag cactatgtaa ggggaaacag
     3181 gtcatcagta ggtggcgaat cggttaccga aaaattctat gatgaccagg aatatcatct
     3241 cagagaagga gatctggtct tcctcttcac cgacggttac tgtgaccagt tcggaggaac
     3301 aggaggcaag aagatgaaga tcagccgtct caagactctg atagatgaca tcaccccgct
     3361 gagcgcagtc gaccagcaga gggagattaa ggacttcttc ttcgaatgga tgggcaaagg
     3421 tgaacaggtt gatgatgtcc tatttatggg gatgagagtc tgatcactca ggaaggcagg
     3481 tgccgttttc acccaggacg actatgcagg tggttgcggt gttgctctca ttgccgctcc
     3541 tgtcctttat ccagaaatcg taataaagtg tgtcatccgg attgtaaatc aggtagataa
     3601 gagtaacgtc tatcgtaccc ttggttatct tgttctgccc ttcagcatca atataaggta
     3661 tgcggtattc tgaggggtaa agcgggtctg aaggtgcggc aagttcgaaa gtgccgttcg
     3721 ttttgcggta tagcctgaaa aagagattgc tcgaagggtc aacaccaggt atcactggtg
     3781 gatcgagtcc gaggtccccg tcgccatcct cgaagtaaaa tgtaattttc ccggctttgg
     3841 cgggattgcc gagcatgtct gtcgtatcat aaagagagaa cgcccggaac tcaatcgacg
     3901 gctccggtgg cagcgtctca atgggcccgc aggaagccat catcagacca cccatgataa
     3961 ccgttatgat aattgcattc ttcatatgcg ggtaacacac cctcgcatca aaaatcacac
     4021 caaatgtatt tatttttttc tgaaataaat cctgaccggc gctccggtaa gctggaagtt
     4081 ctccctgagc ctgttctcga cgaacctttt gtaggggtcc ctgatgtact gtggcaggtt
     4141 gcagaaaaag gcgaatgccg gatattctgc cgggagctgg gtcacgtact ttatacgtat
     4201 atgttttccc tttgtcgaag gcggaggagt ttcaccgata atattaagca tcacctcatt
     4261 cagtttcgaa gtggtaatct tcctgctcct gttttcatat acgctcatta tcagctcaag
     4321 caccttgtgg atcctctgct ttgtgattgc cgagatgaaa agtacagggt aatcggtaaa
     4381 aggcgcggtc tttctcctga tctcttcctc gaactgcttt gccgtggtct ggtctttccc
     4441 gatcaggtcc cacttgttaa ccaggatgat gactcccttg ttgtttttct gaatgatgcc
     4501 gaggatgctc atatcctgtg cctctaggcc gcgggtggca tctatcagaa gcaggcagag
     4561 gtcgctctcc tcgatggctc tcacagacct caggattgag tagaactcga tatcctcatc
     4621 cacctttgct ttttttcgca ggccggcagt gtctatcagc aggaagcgat ggttgaattt
     4681 attgtaaaga ctgtgtattg agtcacgtgt tgtgcctgcc acaggggtta caatgtttct
     4741 ctcctcaccc agaagggtgt ttgcgagtga tgacttgcct acgttcgggc ggcctaccac
     4801 ggccaccttt ggtatatcct ccccggcttc ttcgggtgtg tcgggaaggt gcttcacgat
     4861 ctcgtcgagt aactctccgg taccgctacc gttgaccgag cttaccggga agtagtctcc
     4921 cagccccagg ccgtagaact ctgccgcatc aagaaggcgc ttgttgttgt caaccttgtt
     4981 gaccacagtg atcaccttct tctgaacctt tctgagcatt gccgcaactg ccttgtcaag
     5041 ttcatggatg cctgtctcca cgtcaaccat gaagagtatc acgtctgcct cgctgatggc
     5101 aagcttcacc tgcctgttta tctcgctctc gaagacgtcg tcagagtctt ccacgaagcc
     5161 tcccgtgtca accactgaaa actccacccc gttccagatg gctcttccgt agtgcctgtc
     5221 gcgggtgacg ccactctcct cgttaactat ggccttgcgg ctctccgtta aacggttgaa
     5281 gagggttgac ttgcccacat tgggtcttcc gactattgct gctatcttgc tcataaacaa
     5341 taaaatctac aggtatccga attttttcag catgcgggga ttgtcacgcc attcatgagc
     5401 caccctcaca tacagctcaa ggaagacttt ctttccgagg aactcttcca tctcctttct
     5461 ggaggccgtg ccaacacgtt tgagcattga gcctttatgg ccgatgataa tccccttctg
     5521 gctgtcgcgg gctacatgga tgacggcggc tatccggtta atctcaggct cctcgcggta
     5581 actctcgatc tccacttcca ctgaataggg aatctccttc tggtatgtct cgaagatctt
     5641 tcccctgatg atttc
//
LOCUS       DAOPXU010000145         5632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_17975_length_5632_cov_17.519993, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000145 DAOPXU010000000
VERSION     DAOPXU010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17975_length_5632_cov_17.519993"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..71)
                     /locus_tag="PKK03_11245"
     CDS             complement(<1..71)
                     /locus_tag="PKK03_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNX85015.1"
                     /translation="MGQKVNPIGNRVGIIKGWDSNWYG"
     gene            complement(75..494)
                     /gene="rplV"
                     /locus_tag="PKK03_11250"
     CDS             complement(75..494)
                     /gene="rplV"
                     /locus_tag="PKK03_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNX85016.1"
                     /translation="MGARKRISAVARKEAAKEKAQALLRNVPTSPRKMRLVTDLIRGQ
                     EVNRALDILKFSKQEASRRLEKLLLSAIANWQAKNEGVRLEDSNLYVKEVQVDGGRIL
                     RRIQPAPQGRAYRIRKRSNHVTLVLGSRKQEVENISE"
     gene            complement(529..795)
                     /gene="rpsS"
                     /locus_tag="PKK03_11255"
     CDS             complement(529..795)
                     /gene="rpsS"
                     /locus_tag="PKK03_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNX85017.1"
                     /translation="MSRSIKKGPFIDIHLGKKVDTMNDSGKKTVIKTWSRRSVISPDF
                     VGHTLAVHNGNKFIPVYITENMVGHKLGEFAPTRTFRGHGGKQK"
     gene            complement(804..1628)
                     /gene="rplB"
                     /locus_tag="PKK03_11260"
     CDS             complement(804..1628)
                     /gene="rplB"
                     /locus_tag="PKK03_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNX85018.1"
                     /translation="MAVRKIKPVTPGQRHKITSTFDEVTREMPEKSLLAPLKKTGGRN
                     VNGRRTMRYIGGGHKKMYRVIDFQRTKDGVAAKVTSIEYDPNRSARIALLTYEDGEKR
                     YIIAPNGLKVGQELMSGKTATPEVGNTLFLEDIPLGTIVHNIELKPGRGAAMARSAGT
                     FAQLAARDGKYAIIKLPSGETRMVLTSCRATIGTVSNTDHNQERSGKAGRKRWLGRRP
                     RTRGVAMNPVDHPMGGGEGRASGGHPRSRSGMPAKGFKTRSRTKYSNRHIIERRKK"
     gene            complement(1635..1925)
                     /gene="rplW"
                     /locus_tag="PKK03_11265"
     CDS             complement(1635..1925)
                     /gene="rplW"
                     /locus_tag="PKK03_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNX85019.1"
                     /translation="MEILIKPIVTEKMTAQGEKFNRYGFLVDRKANKLQIKKAVEELY
                     GVNVESVNTINYGGKSKTRYTKSGVMAGRTAATKKAVVTLAKGETIDFYSNI"
     gene            complement(1941..2564)
                     /gene="rplD"
                     /locus_tag="PKK03_11270"
     CDS             complement(1941..2564)
                     /gene="rplD"
                     /locus_tag="PKK03_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNX85020.1"
                     /translation="MEIAVINIDGKETGRKVSLNEAIFGIEPNDHAIYLDTKQYMAGQ
                     RQGTHKAKERAEVMGSTRKLKRQKGTGTARFGSIKNPQFRGGGRIFGPRPRFYGFKLN
                     KKVKELARKSALSYKARENKIIVLEDFSLDVPKTSKMAGMSANLNLPNKKSVIVLPEQ
                     NKNIYLSSRNLQGVEVVTISDLNTYTIMKASTLVLTESAVDVLQSTL"
     gene            complement(2564..3181)
                     /gene="rplC"
                     /locus_tag="PKK03_11275"
     CDS             complement(2564..3181)
                     /gene="rplC"
                     /locus_tag="PKK03_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNX85021.1"
                     /translation="MQGLIGKKVGMTSVFNEDGKSIPCTVLEVGPCVVSQVKTVEKDG
                     YSALQLSFDDKKEKASNKAEIGHFKQAGTGPKRKIAEFVGFEEGKYKRGDVITVDLFN
                     DDTYLDVRGWTKGKGFQGVIKRHGFGGVGGTTHGQHNRLRAPGSMGASSWPSRVMPGM
                     RMAGQKGGKQQETELMIVKIFPESNLLLVKGAVPGPKGGYVIITK"
     gene            complement(3202..3507)
                     /gene="rpsJ"
                     /locus_tag="PKK03_11280"
     CDS             complement(3202..3507)
                     /gene="rpsJ"
                     /locus_tag="PKK03_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNX85022.1"
                     /translation="MSQKIRIKLKSYDHNLVDKSAEKIVKTVKSTGAVVSGPIPLPTH
                     KKIYTVLRSPFVNKKSREQFELSSYKRLLDIYSSTPKTIDALMKLELPSGVEVEIKV"
     gene            complement(3513..5576)
                     /gene="fusA"
                     /locus_tag="PKK03_11285"
     CDS             complement(3513..5576)
                     /gene="fusA"
                     /locus_tag="PKK03_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNX85023.1"
                     /translation="MAHIDAGKTTTSERILYFTGRTHRIGEVDDGNATMDWMVQEQER
                     GITITSAATTTFWKYRDQLFKINLIDTPGHVDFTMEVERSLRVLDGAVAVFCAVGGVE
                     PQSETVWRQANKYNVPRIAFVNKMDRSGADFLGVVQQMKDKLGAHPVPVQIPVGSEDH
                     FDGIIDLVEMNAIMFEESDDPLMNFTVKPIPDFMMEEATEWRMKLVEACAEMDDSILE
                     RYLEDHESITADELMAVLRRETVEGVIVPVFCGAAFKNKGIQRLLNGVVAYLPSPVDV
                     GAVNGKDPRDDKEVTRKPEADEPFAALAFKIATDPYMGRLVFMRVYSGTLNAGDTVLN
                     VRTGKRERINRLFQMHANKQNPVETVYAGDICAGVGFKDLKTGDTLCAINKPVVLESM
                     EFPEPVIGIAIEPKTQADIDRLGVALGKLAEEDPTFRVKTDPDSGQTVISGMGELHLE
                     ILIDRLKREFNVECNQGRPQVAYKEAVTTSYTHRELFRKQTGGRGKFADITVEIMPAE
                     DGVPGLTFVNEIRGGNVPKEYIPSVEKGFREAMMNGPLAGFPLDSLKVILKDGSYHPV
                     DSDSLSFEIAARQAFRHAAAKCRPVLLEPVMNAEVVTPEEYVGDVVGDFNKRRGKVEG
                     MESKAGARVIKAKVPLAENFGYVTVLRTLTSGRATSTMEFSHFEEVPAELAKKIIEVT
                     TGKIL"
BASE COUNT         1104 a         1518 c         1367 g         1643 t
ORIGIN      
        1 ccataccagt tggagtccca tcccttgatg attcctactc tgttgcctat cggatttact
       61 ttctgtccca ttgtttactc tgaaatattt tcaacttcct gtttcctgct acccagcaca
      121 agggttacat ggtttgatct cttgcgtatg cggtaagccc ttccctgggg ggcaggctgg
      181 atacggcgca gaatgcggcc gccgtcaacc tgaacctctt taacgtaaag gttgctgtct
      241 tcgaggcgga ctccttcatt cttggcctgc cagttggcga tggccgagag gagaagtttt
      301 tccagtctgc gtgaagcttc ctgcttgctg aacttcagga tatcaagagc cctgttcact
      361 tcctggccac ggataagatc ggtaaccagc ctcattttgc gcggtgaggt cgggacattg
      421 cgaagaagtg cctgagcctt ctctttagcg gcttccttcc ttgcgactgc gcttattctt
      481 tttcttgcac ccatttctga ttctttttca gtttttatgc cacattacct acttctgttt
      541 tccaccatga ccgcggaagg tgcgggtagg ggcaaactca cccagcttat gaccaaccat
      601 gttctcggta atgtaaacag gtatgaattt gtttccgtta tgcactgcca gggtgtgacc
      661 cacaaagtcg ggtgaaataa ctgatcttct cgaccaggtc ttgatcacag ttttctttcc
      721 gctgtcattc attgtgtcca cctttttgcc caggtgaata tcgatgaatg ggcctttttt
      781 aatagatctg ctcatcgtat caattatttt ttccttcttt caataatatg cctgttagaa
      841 tacttggtcc ggctcctggt tttgaaaccc tttgcgggca taccgctgcg tgagcgcggg
      901 tgtccgccgg aggctcttcc ttcaccgccg cccattggat ggtcaaccgg gttcatggca
      961 acgcccctgg ttcttggcct gcggccgagc cagcgtttgc ggcctgcctt tccggatctc
     1021 tcctggttgt ggtcagtgtt agaaacagtt cctatcgttg cgcggcatga ggtaagcacc
     1081 atcctggtct cccccgaagg aagcttgatg attgcatact tgccgtcacg ggcagccagc
     1141 tgtgcaaaag tgcctgcact gcgggccatt gctgcacccc ttccgggctt cagctcaatg
     1201 ttgtgaacta tggtaccgag aggaatgtct tcaaggaaga gggtgtttcc aacctcaggg
     1261 gttgccgtct ttcctgacat cagctcctgg ccgaccttca ggccgttggg ggcaatgata
     1321 tacctcttct ctccgtcttc ataggtgagg agggcaatac gtgccgaacg gttcgggtcg
     1381 tattcgatcg atgtgacctt cgcggcaacg ccgtccttgg tcctctggaa atcgatcaca
     1441 cggtacattt ttttgtggcc gccgccgatg tacctcatgg tccgtctgcc gttaacgttc
     1501 cttcctcctg ttttcttcag cggtgcgagc aaggatttct ccggcatctc acgggttacc
     1561 tcgtcaaagg tactcgtgat cttatgtctc tggcctggtg ttacaggctt gatctttctg
     1621 actgccatat tatattaaat gttgctgtaa aaatctatgg tctctccctt ggcgagagtg
     1681 acaacagcct ttttcgttgc tgccgtccgg cctgccatta caccggactt ggtgtagcgg
     1741 gtcttgctct tgccaccgta gttgatggtg ttgacgctct caacattgac cccgtaaagc
     1801 tcctcaacag ctttcttgat ctgaagcttg ttggccttac ggtcaaccag gaagccatac
     1861 cggttgaact tctcgccctg ggcggtcatt ttctcggtca ctatgggttt gataagtatt
     1921 tccatatctc tgctcctggt ttataatgtt gactgcaaaa catcaactgc gctctctgtc
     1981 agcactagag ttgaagcttt cataattgta taagtgttta aatcactgat agttacaact
     2041 tcaactccct gcaaatttcg agatgacaaa tatatatttt tattttgttc cggtaaaaca
     2101 atgactgatt ttttattcgg gaggttgagg tttgcgctca tcccggccat ttttgaagtc
     2161 ttcggaacat cgagtgagaa gtcttcaagg acgatgattt tgttttctct tgccttgtat
     2221 gagagggctg atttcctggc cagttccttt acctttttgt tgagtttgaa gccgtagaat
     2281 ctgggtctgg ggccgaagat gcggccgccg ccacggaact gagggttctt gatgctgccg
     2341 aaacgtgctg ttccggtgcc tttctgtctc ttgagcttgc gtgtgctgcc catgacctct
     2401 gcgcgttcct tggccttgtg tgtgccctgt ctctggccgg ccatatactg tttggtatca
     2461 aggtaaatag cgtggtcatt gggttcaatg ccgaagatgg cctcattcag actgaccttt
     2521 cttccggtct ctttgccgtc tatattaatt actgctattt ccattacttt gttattatta
     2581 cgtaaccacc tttggggccg ggtacggcac ccttgacgag aagcaggttt gattcgggga
     2641 agatcttaac gatcatcagt tctgtttcct gctgttttcc gcctttttgt cctgccatgc
     2701 gcattccggg cataaccctt gacggccatg aagatgcgcc cattgaaccg ggagccctga
     2761 gcctgttgtg ctggccgtgg gttgtaccgc cgacgccgcc gaagccgtga cgcttgatga
     2821 cgccctggaa gcctttgccc ttggtccagc ccctgacatc aagatatgta tcatcattga
     2881 agagatcgac tgtaatgaca tcgcctctct tgtatttgcc ttcctcgaat ccgacgaact
     2941 cggcaatttt cctcttcggg cctgtgccgg cctgcttgaa atgacctatc tcagccttgt
     3001 tgcttgcctt ttccttcttg tcgtcgaaac tgagctgcaa agccgaataa ccgtctttct
     3061 ccacagtctt tacctgagac acgacacacg ggcccacttc caggactgtg catggaatgc
     3121 tcttcccgtc ttcattgaag acggaggtca ttccaacctt ttttcctatt aatccctgca
     3181 taattcagta tttttccggt ttcacacttt aatttccact tccactccgc tgggcagctc
     3241 aagcttcatc agagcatcga tggttttggg tgttgagctg tatatgtcaa gcaggcgctt
     3301 gtacgatgac agttcgaact gctctcttga tttcttgttc acgaacggtg aacggagcac
     3361 cgtatagatc ttcttgtggg tgggcaacgg tatgggaccg ctgacaactg cacccgtcga
     3421 tttgaccgtc ttgacaatct tctcagccga cttgtcgacc aggttatgat cgtaagactt
     3481 cagtttgatg cggattttct gactcatctt tgttaaagaa tttttcctgt tgttacttca
     3541 ataattttct tcgctaattc tgcaggcacc tcttcaaaat gcgagaactc cattgtagat
     3601 gtagccctgc ccgaggtaag ggtacgcagt actgtaacgt atccgaagtt ttctgccaga
     3661 ggtacttttg ctttaattac ccttgcacct gctttcgatt ccataccttc caccttgcct
     3721 cttcgcttgt tgaagtcgcc cacgacgtca cccacatatt cttccggggt aactacctcg
     3781 gcgttcatca ccggttcgag cagcaccggg cggcatttgg ccgccgcatg gcggaaggcc
     3841 tgcctggccg caatctcgaa cgaaagcgaa tctgagtcaa ccggatggta cgaaccgtct
     3901 ttgaggataa cctttaggct gtcgaggggg aagccggcca gcgggccgtt catcattgcc
     3961 tcgcggaatc ccttttccac tgaagggata tattcctttg gcacattgcc tcccctgatc
     4021 tcgttcacga aggtcagtcc cggaacacca tcttcggcag gcatgatctc cacggtgata
     4081 tctgcgaatt tgcccctgcc gcctgtctgt ttcctgaaga gttccctgtg tgtgtaggag
     4141 gttgtgacgg cctccttgta tgccacctgt ggtctgccct ggttgcattc aacattgaac
     4201 tctctcttaa gacggtcaat aagaatttca agatgcagct cacccatgcc actgatgact
     4261 gtctgtccgc tgtcaggatc tgtcttcacc ctgaaggtgg gatcctcttc ggcaagtttg
     4321 ccgagtgcaa cgcccagacg gtcaatatca gcctgtgttt ttggctcaat ggcaatcccg
     4381 ataaccggtt ccgggaattc cattgattca agcaccaccg gcttgttaat ggcacagagt
     4441 gtatctccag tcttgaggtc cttgaatccc acgcctgcgc agatgtcacc tgcatagacg
     4501 gtttcaacgg gattctgttt gtttgcatgc atttggaaga ggcggttgat cctctccctc
     4561 ttgccggtcc tgacattgag aacggtgtct cctgcattga gggtaccgga atagaccctc
     4621 atgaagacca gccgtcccat gtacgggtcg gtagctattt taaaggcgag agcagcaaaa
     4681 ggctcgtcag cctcaggttt gcgcgtgacc tccttatcat cacgcgggtc tttgccattt
     4741 acggcaccca cgtcaaccgg ggagggcagg tatgccacca ctccgttcag gaggcgctgt
     4801 atccccttgt tcttgaaagc agctccgcag aagacgggaa ctattactcc ctctactgtc
     4861 tcgcggcgga gcactgccat aagctcatca gcagtgatgc tctcatggtc ttcgaggtaa
     4921 cgttcgagga ttgaatcatc catctcggcg catgcctcaa ccagcttcat tctccactcg
     4981 gttgcctctt ccatcatgaa atcggggata ggcttgacag tgaagttcat gagcgggtca
     5041 tcactctcct cgaacataat ggcattcatc tctacaaggt caatgatgcc gtcgaaatga
     5101 tcctcagaac cgactggtat ctgtacaggc accgggtgag ccccgagctt atccttcatc
     5161 tgctgaacca ctccgaggaa atcggcgccg gaacggtcca tcttgttgac aaacgctatg
     5221 cgcggcacat tatatttgtt agcctgcctc cagacagtct cgctctgggg ttccacgccg
     5281 cccacggcgc agaagaccgc caccgcaccg tcgagtacgc gcagagaccg ttccacctcc
     5341 atggtgaagt ctacgtgccc cggagtgtca atgaggttga tcttgaagag ctgatcccgg
     5401 tatttccaga atgtggtagt ggcagctgac gtgatagtga tccctctctc ctgttcctgc
     5461 accatccagt ccatggtggc attgccgtca tcgacttcgc cgatgcgatg agtacggccc
     5521 gtgaaataga gaatgcgctc ggaagttgta gtcttcccgg catcaatatg ggccattatg
     5581 ccaatgttcc tggtattttt gagatcgctg ttcattctgt gctcccggct aa
//
LOCUS       DAOPXU010000146         5609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_18079_length_5609_cov_12.267375, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000146 DAOPXU010000000
VERSION     DAOPXU010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18079_length_5609_cov_12.267375"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /locus_tag="PKK03_11290"
     CDS             complement(<1..1523)
                     /locus_tag="PKK03_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HNX85024.1"
                     /translation="MKGNIEIKGARVHNLREISLSIPRNSLVVITGVSGSGKSSLAFD
                     TIYAEGQRRYVESLSSYARQFLGRMNKPDVDFINGIPPAIAIEQKVNTRNPRSTVGTS
                     TEIFDYLRLLWARVGRTISPVSGREVRRHTVDDVVNYLATLTPGTRVVLTAPIAVPEN
                     VDPAEYIKFLIQQGYNRISTEKGVIRLDDAGTAELTAGMRSAELIIDRFAAGDSPEEL
                     SRAADSVQTAFLTGRGEINLTVTDEETTQTVRFSDRFEEDGITFEEPTEHLFSFNSPL
                     GACPVCEGYGKIIGIDESLVIPDENLSVYDNAIACWRGEIMKQWKDRLIYSADKFDFP
                     IHKPWYKLTPEQKALVWNGNRYFFGLNRFFEHLEEKKYKIQYRVMLSRYRGKRSCPEC
                     GGMRLRREAGYVKVGGRSVTEVTSIPISEAHSFFGHLVLNGEEKEIAGRLLKEIQQRL
                     GFLVDVGLSYLTLDRLSNTLSGGESQRINLATSLGSSLVGSLYILDEPSIGLHPRDTA
                     "
     gene            1721..2308
                     /locus_tag="PKK03_11295"
     CDS             1721..2308
                     /locus_tag="PKK03_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX85025.1"
                     /translation="MKSKMTDYELITSFMNGNERSIELLILRHKSKVYSYISLYIRNR
                     DLADDIFQDTFLKVVQSIRSGRYQDDGKFISWVMRIAHNLIIDHFRHEKQMGIVLNDD
                     YETDLFNSKRLTDDNVEDVIVRRQVMRDVRRLVNGLPEDQREVVIMRHYAGMSFKEIA
                     DMTGVSINTALGRMRYALINMRKIMVEKNITLSIS"
     gene            2361..2546
                     /locus_tag="PKK03_11300"
     CDS             2361..2546
                     /locus_tag="PKK03_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85026.1"
                     /translation="MDKNSTLLLSYFEITNTPSDLISDAFGIDNALSVLLPDMDDTPS
                     DEVIASLFEKIRKDKVN"
     gene            2683..4353
                     /locus_tag="PKK03_11305"
     CDS             2683..4353
                     /locus_tag="PKK03_11305"
                     /inference="COORDINATES: protein motif:HMM:NF013560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HNX85027.1"
                     /translation="MKRSIVYFTALMTLLWSCTSKQEKMENRMRAFIASYETAVIPLY
                     KESALSSWDANVTGTDEAWARNEKASLAYISYFTDKKAFDELKELKASGMVKDPVLAR
                     QLEVIYNQFLSGQVDTSLLAEQVRMETEITKKFNSFRADVSGKMLSDNEVEDILRNST
                     RSAELQAAWEGHKKIGPVVADDIIKLVRHRNRIAQELGFSNYHQMSLKLSDQDPEEVT
                     RIFDELDSLTRGTFATLKDEIDNYYSKRYGIPAADLMPWHYQNRYFQEAPEIYSVDFD
                     SYYKSKDPVKLAATYYASIGLPVDEIIARSDLYEKPGKNQHAFSTDIDREGDVRTLDN
                     IRPDSYWMNTIMHELGHGVYSYYNDRALPFAIRDAAHTFTTEAVANFFGRYATDPAWM
                     QLMGLIDAKEAEKISKDAFNAFRLQQLVFSRWAQVMYRFEKAMYENPDQDLNQLWWDM
                     VEKYQMMKEPAGRDMPDWATKIHVALYPCYYHNYLLGELLASQFSAYITGSVVKGNAG
                     EDMKAMGEFFKIKVFMPGMRYEWNEMIEKATGEKLTARYYAAQVVGEK"
     gene            4370..>5609
                     /locus_tag="PKK03_11310"
     CDS             4370..>5609
                     /locus_tag="PKK03_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85028.1"
                     /translation="MRKVIFLMAIVLTACSTPAKKGGETASAFIGETAVTSAIDRVMG
                     EQTAADRLLVEKGVRHAASLWREEDGTAEEFADFASANYISDPAKRKGVFIKISNYLE
                     SLAGNMNEITLDLKKILDEAAGDIDEVDRMFGNYSVGSHLQSDFYENKIAFVIALNFP
                     YYTLEEKDKLGSGWSREQWAMARLGDQFVSRVPAELNQKANTALGNAEMYIAEYNIHM
                     GKLRTDDGRQLFADDMVLLSHWNLRDEIKSNYADMETGNEKQEMVYKVMERIIRQEIP
                     AKVINNPQYEWAPYSNKVTKDGAAADASAEPDTRYSHVISIFKAWKAIDPYNPGMNTA
                     MLRSFSGGMEISEEDAAALFNEYLSSPQLRQLAEIIRERLGRDLRPYDIWYDGFKSRS
                     SMPESMLTAKTSALYPDPAAF"
BASE COUNT         1484 a         1350 c         1422 g         1353 t
ORIGIN      
        1 gcggtgtcac gagggtggag tccgatgctg ggttcgtcaa ggatataaag agagcccacc
       61 aggctgcttc cgagagaagt ggccaggttg atgcgttgcg attcaccacc cgagagggtg
      121 ttggacagcc tgtctagggt aagataggag agccccacat ccacgaggaa tcccagccgt
      181 tgctgtatct ccttcaggag gcggcctgca atctccttct cctcaccgtt gagtaccagg
      241 tgtccgaaga agctgtgcgc ttcgctgatg ggaatggagg tgacctcagt tacagacctg
      301 ccgccaactt taacgtagcc cgcctccctg cgcagacgca tccctccgca ttcaggacat
      361 gaccgtttgc cccggtagcg gctgagcatg accctgtact gtatcttgta ttttttctct
      421 tcaaggtgct cgaagaagcg gttcagcccg aagaagtacc tgttgccgtt ccataccagc
      481 gctttctgct caggtgtcag tttataccag ggcttatgaa tgggaaagtc gaatttgtcg
      541 gcactgtaga tgagcctgtc cttccactgc ttcattattt ccccgcgcca gcaggctatg
      601 gcattgtcat agacagagag attttcatca gggatgacca ggctctcgtc tatccctatt
      661 attttcccgt agccctcgca gacagggcat gctcccagcg gagagttgaa gctgaagaga
      721 tgctctgtcg gctcctcgaa ggttataccg tcttcctcga acctgtcaga gaacctcacc
      781 gtctgggtcg tctcctcatc ggtcacggtc aggtttattt cgcctcttcc cgtcaggaat
      841 gccgtctgca ccgagtcggc cgcacgactg agttcctccg gtgagtcacc ggcagcaaac
      901 ctgtcaataa ttagctccgc gctgcgcatc cccgccgtca gttctgccgt tcctgcatca
      961 tcaagtctta tgacaccctt ttcggtgctg atacggttgt atccctgctg gataaggaat
     1021 tttatatatt cagccggatc tacattctcc ggcactgcaa tgggagcagt aagcaccacc
     1081 cttgttccgg gagtcagagt ggcaaggtaa ttgacaacgt catcaacggt gtgacgccgc
     1141 acttcccgtc ctgataccgg agaaattgtc cgtcccacgc gggcccagag aaggcgcaga
     1201 tagtcgaata tctccgtgga ggttcctacg gttgaccttg gattccttgt gttgactttt
     1261 tgttctatag cgatggcagg agggatgccg tttatgaaat caacgtcggg tttgttcatc
     1321 ctgcccagga actgccgggc ataggaggag aggctttcca catacctgcg ctgaccctcg
     1381 gcatatattg tatcaaatgc cagtgaagac ttgcctgatc cggagacgcc ggtgatgacc
     1441 acaaggctgt ttcgtggtat tgagagggaa atctccctga ggttgtgcac acgcgctccc
     1501 tttatctcaa tattgccctt cattctgccc tggttgataa gttggcaaaa ttaacaattt
     1561 tgaatttact attttttgtt ttctttgtct cagtcagaaa tgaaacaaaa cggaatacca
     1621 ccccgtataa gccatcagtt aacaagaaaa aaaccaacaa cgaaacaaag gaggaccgcc
     1681 tatcgtataa ctctacccga aaccaataat agagttagat atgaagagca aaatgaccga
     1741 ttatgagctg attacaagct tcatgaacgg taatgagaga agcatagagc tgctcatcct
     1801 ccgacacaaa agtaaagtct attcctatat aagcctttac atccgcaaca gggacctggc
     1861 cgatgatatt ttccaggaca ccttcctcaa agtagttcag tcaatacgct cgggccgtta
     1921 ccaggacgac ggcaagttta tctcctgggt gatgaggatt gctcacaacc ttattattga
     1981 tcacttcagg catgagaagc agatggggat tgtcctcaat gatgattatg agactgatct
     2041 cttcaactcg aagaggctga cagatgacaa tgttgaggat gtaatcgtac gtcgccaggt
     2101 tatgcgtgat gtaaggcgcc ttgtcaacgg gctccccgag gatcagcgtg aggtggttat
     2161 catgcgtcac tatgccggaa tgagcttcaa ggagattgct gacatgaccg gagtaagcat
     2221 taacaccgcg ctgggacgga tgagatacgc acttatcaac atgcgcaaga taatggttga
     2281 aaaaaatatc accctttcaa tcagctgata caatttttga tgtaaggggg cgtttttctg
     2341 atatgagaac agagacgcct atggataaga attctaccct cttactttca tattttgaga
     2401 tcaccaacac cccgtcagac ctgatttcag atgctttcgg catcgataat gccctgagcg
     2461 ttctgcttcc tgacatggat gatacaccgt ctgatgaggt cattgccagc ctgttcgaga
     2521 agatcaggaa ggataaggtg aactaagaaa ctttcctcaa atactttcaa cattggtcgg
     2581 agaaccggca ggcaggtcct gtccggttct tccaatatct gaccggcatc atgatcattc
     2641 ttgcatgatt tttttacctt agcgccactc aattaaaaat ttatgaaaag atcaattgtc
     2701 tatttcactg cactgatgac cctattgtgg tcatgcactt caaaacagga aaagatggaa
     2761 aacaggatga gggctttcat tgcctcctac gagacggctg taattcccct atacaaggaa
     2821 tcagctctct cttcatggga tgcaaatgtc accggtaccg acgaggcatg ggcaagaaac
     2881 gaaaaagcct ccctggctta tatcagctac ttcactgata aaaaggcatt cgacgagctt
     2941 aaggaactga aggcatcggg aatggtgaag gatccggttc tggcacgaca gctggaagtg
     3001 atctacaacc aattcctttc aggccaggtt gacacgagcc ttcttgcaga acaggtgagg
     3061 atggagacgg agataacaaa gaaattcaat tcattcaggg ctgatgtcag cggaaagatg
     3121 ctgagcgaca atgaggtgga ggatattctt cgcaactcca cccggtcagc cgagcttcag
     3181 gctgcatggg aaggccataa aaagatcggc ccggttgtgg ctgatgacat aataaagctt
     3241 gtcaggcacc ggaacaggat cgcacaggag ctgggtttca gcaactatca ccagatgagc
     3301 ctgaaactgt cagaccagga tcctgaggag gttacaagga tttttgatga gcttgacagt
     3361 ctcacaagag gtactttcgc taccctcaaa gacgagatag ataattatta ttcaaagcgt
     3421 tacggcattc cggccgctga cctgatgcca tggcattacc agaaccggta cttccaggaa
     3481 gcgccggaaa tctactctgt cgattttgac agttattata agagtaagga tcccgttaag
     3541 cttgcagcaa cctactacgc atccatcggg ctgccggttg atgaaataat tgccaggagc
     3601 gatctttatg aaaagcccgg caagaaccag cacgcattct ccacagacat cgacagggag
     3661 ggtgatgtcc gcacccttga caacatcagg cctgattcat actggatgaa caccataatg
     3721 catgagcttg gccatggtgt atattcgtac tacaacgaca gggccctgcc atttgccatc
     3781 agggatgctg cccatacatt caccacagag gctgtggcca atttctttgg cagatatgca
     3841 actgatcctg catggatgca gctcatgggt ttaatagatg cgaaggaagc agagaagata
     3901 agcaaggacg ccttcaatgc cttcagactg caacaacttg ttttcagccg ctgggcacag
     3961 gtgatgtaca ggtttgaaaa agccatgtat gaaaatccgg atcaggatct caaccagttg
     4021 tggtgggaca tggttgagaa ataccagatg atgaaggaac cggcagggcg tgacatgcct
     4081 gactgggcca ccaagataca tgtggctctc tacccgtgct attaccataa ctaccttctt
     4141 ggcgaacttc ttgcttcaca gttctctgca tatatcacag gctcggttgt aaagggcaat
     4201 gccggcgagg atatgaaggc catgggtgaa ttctttaaaa taaaagtatt tatgcccggc
     4261 atgcgatatg agtggaatga aatgatagaa aaggcaacag gagagaaact gactgccagg
     4321 tactacgctg cccaggttgt cggtgaaaaa taatcataaa ttgatagaaa tgagaaaagt
     4381 aattttcctt atggccattg tattgacagc atgttcgacc cctgcaaaaa agggtgggga
     4441 gacagcatca gccttcatcg gtgaaacggc agtcacctct gccatcgacc gtgtaatggg
     4501 agaacagacc gctgccgaca ggttacttgt cgaaaagggt gtgcgtcatg ccgcctcgct
     4561 ctggcgcgaa gaggacggaa ctgctgagga atttgccgat ttcgcctccg cgaactatat
     4621 ttctgatccg gccaaacgga agggtgtgtt catcaagatc agcaactatc ttgagtcact
     4681 ggccgggaac atgaacgaga tcacactcga cctgaagaaa atcctcgacg aggctgccgg
     4741 tgatatcgat gaggtagacc gcatgttcgg aaactattcc gtgggctctc accttcagtc
     4801 ggatttctat gagaacaaga ttgcatttgt gattgcgctc aacttcccgt attatacact
     4861 tgaggagaag gataaactag gctccggctg gagtagggag cagtgggcca tggcccggct
     4921 gggtgaccag tttgtgtcac gcgtgcctgc ggagctgaac cagaaagcga acactgcact
     4981 gggaaatgcc gagatgtata ttgcagaata caatattcat atgggaaagc tccgcaccga
     5041 tgatggccgg cagcttttcg ccgatgatat ggtgcttctc tctcactgga acctccgaga
     5101 cgagatcaag tcgaactatg ccgacatgga aaccggaaac gagaaacagg agatggttta
     5161 caaggtgatg gaaaggataa tcaggcagga gattccggca aaagttatca ataatcctca
     5221 gtacgagtgg gccccttact ccaacaaagt gacgaaggat ggtgcagcgg cagatgcctc
     5281 tgctgagccg gatacccgct attctcatgt aatcagcatt ttcaaagcat ggaaggcaat
     5341 tgacccgtac aatcccggca tgaataccgc catgctgcgc agtttttccg gagggatgga
     5401 aatatcagag gaggatgctg cagccctgtt caatgagtat ctcagctcac ctcagctcag
     5461 gcaactggca gaaattataa gggagaggct cgggcgtgat ctcaggccgt atgacatatg
     5521 gtatgacggc tttaagtcga ggagctcaat gccggagagc atgctcaccg caaagacatc
     5581 tgccctttac cctgatcctg ctgccttca
//
LOCUS       DAOPXU010000147         5555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_18346_length_5555_cov_9.574182, whole genome shotgun sequence.
ACCESSION   DAOPXU010000147 DAOPXU010000000
VERSION     DAOPXU010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18346_length_5555_cov_9.574182"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3345)
                     /locus_tag="PKK03_11315"
     CDS             complement(<1..>3345)
                     /locus_tag="PKK03_11315"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNX85029.1"
                     /translation="TLSPANRSRALISGSFMYEYYDLRSLKLVVRTPDSVQYRGRPFI
                     VGLKALNENDMILPDARVTLHILRDRVNEIPGPSLPVKDTLAVIRENLRPAGETVVMI
                     PDSIFPAANMTCRIVAIANTSDNESVTETKSMTFIDRKEEICYSTRADSIKFYLKVNG
                     TETEREAELITSDAFGNKKPASKISLPWQIKTDPFITDYAITSGNARRTVETSELPHG
                     IAPRTFRKADSVFISVTSNTGLAFSYFIYDINREVARGTAETLEFRERVTSDRKWYLS
                     LSYLWGGVMNNSIYEISRDISKLNILAEQPDKIIPGKETAITLTVTDAKGSPVRNTDI
                     TAFSITRKFGYNLPALPFLAKPRKSKEQINSFRTSTPNAPYLAHQLEYDWWNAKAGLD
                     TVEYFRFRYHPEEIAIFHCDMGDSITQFAPFIFRDGQPVRINQISVDHRPVYIDFASA
                     NQPYSFRVDTGYHFISIRTPEAIYEADSVRFERGRKLVLSIRDTEMPEGYYKKDASPK
                     MQVSEQRRIANYVMPYRTLFENNVAYLQQGDNLLLLSNVNQQLANPDRVFTSQGTRMV
                     GPVMPTNARFVLPGKFTTDFRFEPLYEYDFEKGLMRMRSFDPAKRIPLYYAKGNTMQD
                     WRSSVLTPAYIEKVSREIMEKWTAPRYRYSRSERTVPGNGTVQILDSRPEKVASPVVN
                     FLVSVAGRAVYDRQGYDKALTNIESGIYRFISFFPGGHQSVIDSVEVRSNSTTYIDMA
                     WADTSDDPELYNKIIEMINTPGSRPGTPSSLERQLMQEFTRQDVNAYDGPGYTVSGVV
                     TSLSDNERLPGVTVLCQENGFGTITDIDGRYSIRLPFGNHKLSFMFIGMKPYETDVYF
                     ENQLDITLEEEVLHLDEVVVVGYGISRKESLTASVVTVNSLAGRVAGLDVSDGRSIMI
                     RGVSSADAQPPLIIIDGVPWSGDMSLIDPELLKSMTVIKDPAMTALYGSRAAGGVLML
                     TMKPGGVIAAAAESDPLVDEQFLEEAMAAGTLRRNFRDYGYWKPDLRTDDSGKVTFNV
                     KFPDDITSWETFAVAINGNRQAGSAKGNIRAFMPVTGLLSAPHYVVEGDSLNLIGRIM
                     NHTGAPMSLTERFISNGDTLIDRHRD"
     assembly_gap    3346..3445
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3446..3728)
                     /locus_tag="PKK03_11320"
     CDS             complement(<3446..3728)
                     /locus_tag="PKK03_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85030.1"
                     /translation="MTRLLTFLPALLITFTLGSQDLAESRTTSPFTYIFSITEKQALD
                     LIRKSSTGKDQELFRTVVDSFPTGTKFKGKLKPGNYVTAYVETDKVRVLY"
     gene            3928..5187
                     /locus_tag="PKK03_11325"
     CDS             3928..5187
                     /locus_tag="PKK03_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85031.1"
                     /translation="MKKILLTGLILLGYSAVSGQTVADTLRKDALNVFMEASDYVRKE
                     IPYVNYVRDIKDAGLYIISTTQMTGSGGREFTYFFIGQNENQGMRDTLKFTSSPDETS
                     ELIRENEVRTLKLGLIRYVARTPLARFLKISFSEPLAQTVTTDKWNSWVFKTSVSGYA
                     SGEKSYRSSYLNGSFSANRVTEKLKSSVSLRYSYSSDKYEVDDELITSEYNSKSLSGL
                     LVGSLTDHWSVGGSMYAGASRYNNSDLSFNIQPGIEYNVFPYSESTRRQLRITYKLGL
                     GYENYIDTTIYNKVKENLFSQYLTVNYEVIQKWGSANLSLGYSNYLHDWSKNNISLNA
                     FLDLRIAKGLSLNFGGGGAIVHDQLGLVKGGATQQEVLLRLRELETQFEYFTSFGLTY
                     TFGSIYNNVVNPRFGNSGGGGMTIIMN"
     gene            complement(5304..>5555)
                     /locus_tag="PKK03_11330"
     CDS             complement(5304..>5555)
                     /locus_tag="PKK03_11330"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNX85032.1"
                     /translation="GKALTEGQPEGILRILSEPKYGEVLGVQIISHNATDLISEAAAY
                     MAVESTVWDVAHTMHAHPTISEVFLEAGADAVGKPVHKA"
BASE COUNT         1324 a         1313 c         1341 g         1477 t
ORIGIN      
        1 ggtcacgatg gcggtcaata agtgtgtcgc cgttactgat gaatctttct gtcagcgaca
       61 tgggcgcgcc tgtgtgattc atgatccggc caataaggtt aagcgagtcg ccttcaacaa
      121 cataatgtgg cgccgagagc aacccggtca caggcatgaa tgccctgata tttccctttg
      181 cagagcctgc ctgacggtta ccattgatag ctacggcaaa tgtctcccat gaggtgatgt
      241 catcggggaa cttaacgtta aaggtaactt tacccgaatc atcagtgcgc aggtcgggct
      301 tccagtagcc gtagtcgcgg aagttcctgc gaagggtacc tgcagccatt gcctcttcca
      361 ggaattgctc atccacaagc gggtcacttt ccgcagcggc cgcgatgact cctccgggct
      421 tcattgtaag catgagcact cctcctgcag ctcttgatcc gtacagtgct gtcattgccg
      481 ggtcctttat aacggtcatg ctcttaagca gttcaggatc aatgagactc atgtcaccgc
      541 tccatggcac accgtcaatt ataataaggg gaggctgagc gtcagctgat gagacgcctc
      601 taatcatgat ggacctgccg tctgatacat ccagacctgc caccctccct gcaagcgaat
      661 tgacagttac cactgatgct gtgagacttt ccttccggga aattccataa cccaccacaa
      721 caacttcatc caggtgcaaa acttcttctt caagagttat atccagctga ttttcaaagt
      781 agacatcagt ttcgtatggt ttcatcccga tgaacatgaa tgacagctta tggttcccga
      841 aggggagtct gattgagtat ctgccatcaa tatctgtgat agtaccaaat ccgttttcct
      901 ggcacaagac cgtcactccc ggcagccgct cattgtctga gagacttgta acaaccccgg
      961 agactgtgta tcccggtccg tcataggcgt tcacatcctg ccttgtaaac tcctgcatga
     1021 gttgccgttc cagtgaggat ggcgttccag gccggcttcc cggggtattg atcatctcaa
     1081 tgatcttatt ataaagttcc ggatcatctg aagtgtcagc ccatgccatg tcaatatagg
     1141 tagtgctgtt gctccttact tcgacggagt caataactga ctggtgccca cccggaaaga
     1201 aggagatgaa cctgtaaatt cccgattcga tattggttaa ggccttatca taaccctgcc
     1261 tgtcatagac tgcccttccc gcaacggaga ccaggaagtt tacaaccggg ctggcaacct
     1321 tctccggccg gctgtcaagt atctgaaccg ttccgtttcc gggcacagtt ctctcactgc
     1381 gtgaatacct gtaacggggg gctgtccact tttccatgat ctcgcggctt accttctcaa
     1441 tataggccgg agtcaggaca ctgcttcgcc agtcctgcat cgtattgccc tttgcatagt
     1501 acagcggaat cctttttgca gggtcgaatg acctcattct cattaaacct ttctcaaaat
     1561 catattcata caggggctcg aacctgaaat ccgttgtaaa ttttccaggc agaacgaagc
     1621 gtgcatttgt cggcattacc ggacccacca tccgtgtgcc ttgcgaagta aagaccctgt
     1681 ccggatttgc aagttgctga ttcacattgc tcaacagaag aaggttgtct ccctgctgca
     1741 gataggcaac gttgttttca aacagtgtcc tgtagggcat gacgtaattg gcgatcctgc
     1801 gttgttctga tacctgcatt ttgggagatg catccttttt ataatatcct tcaggcattt
     1861 cggtatccct gatagaaagg accagcttcc tgcctctttc aaaccggacg ctgtcagctt
     1921 cgtatatagc ctcgggggtc ctgatgctta tgaaatggta accggtgtca accctgaagg
     1981 agtatggctg attggccgat gcaaaatcaa tgtaaaccgg cctgtggtca acactaatct
     2041 ggttaattct tacgggctgg ccgtcacgga agatgaacgg ggcgaactgg gtgatgctgt
     2101 cacccatgtc acagtggaat attgcgatct cttcagggtg atagcggaat ctgaaatatt
     2161 cgaccgtatc aaggcctgct ttggcgttcc accagtcgta ttcaagctga tgggcaagat
     2221 atggtgcatt gggcgtggac gtacggaatg agttgatttg ttccttgctt ttcctgggtt
     2281 tcgcaagaaa cggaagtgcc ggcagattgt acccgaactt gcgggtaatg gaaaaagctg
     2341 ttatatcggt attccggacg ggactgccct tcgcatctgt gactgtgagg gtgatggcag
     2401 tctctttccc gggaatgatc ttgtcaggct gctctgcaag tatattcagc ttgctgatat
     2461 ccctggagat ctcgtagatg ctgttgttca tcactccgcc ccacaggtag ctgagtgaga
     2521 gataccattt ccggtcagaa gtgactctct ccctgaattc aagggtctca gcggttcctc
     2581 gtgccacctc gcggttgatg tcatatatga agtagctgaa tgccagtccc gtgttgctgg
     2641 tgacgctgat aaatacggag tcggcttttc tgaatgtccg gggtgcaatg ccgtgcggca
     2701 gctcactggt ttccactgtc cgcctggcat ttcccgaagt gattgcatag tccgtgatga
     2761 acgggtcagt tttaatttgc caggggaggc ttattttcga tgcaggcttt ttgttcccga
     2821 aggcatcaga ggttatcagt tctgcctctc tttcagtttc tgttccattg actttcaggt
     2881 aaaactttat tgaatctgcc cgggtgctgt agcaaatctc ttctttcctg tcaataaagg
     2941 tcatcgattt ggtttcagta acagactcat tgtccgaggt gttggcaata gcaacaatcc
     3001 tgcatgtcat gttggcggca ggaaagattg aatcagggat cattacaact gtctctccag
     3061 ccggacgaag gttttccctt atcacagcaa gagtgtcctt caccggaaga gacggccccg
     3121 gaatctcatt gacccggtca cgcaggatat ggagtgtcac cctggcatca ggcagaatca
     3181 tgtcattttc attcagggct ttaagtccga caataaacgg ccggcctctg tactgaacgc
     3241 tgtcaggtgt cctgacgacg agtttcagac tccggagatc ataatactca tacatgaatg
     3301 atccggagat aagtgcacgg ctcctgtttg caggcgacag ggtaannnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnntataa agcaccctta ccttgtcggt ttcaacatac
     3481 gccgtgacat aattgccggg cttcagctta cctttaaatt tcgttccggt agggaaggag
     3541 tcgaccacag tccggaatag ttcctggtcc ttgccggttg agctcttgcg gataaggtca
     3601 agtgcttgtt tttcagttat tgagaagatg tatgtgaacg ggcttgtggt gcggctttca
     3661 gccaggtcct gtgatcccag tgtgaacgtg ataagcaggg ccggcaggaa agtaagtaac
     3721 cttgtcatgt ggtgacagct ttttaatgca aacaggcata aaagtagcaa ataaagaaag
     3781 gaattgttcc gcagatgacc gctaatccga tttaccggca aaacgaggat aaatgatttt
     3841 gattttgaaa tgatgcatat ttaacgataa ctttggattt catactgtct gaatcatttc
     3901 cgtcaaacaa aaccggggga gaaaacaatg aaaaagatcc ttttaacagg cctgatatta
     3961 ttgggctaca gtgccgtttc aggccaaaca gtggctgata ctctccgaaa agatgccctg
     4021 aatgtgttca tggaagcatc cgattatgta cggaaagaaa ttccctatgt caattatgtc
     4081 cgtgatatta aggacgcggg tttatatatc atatcaacca cgcagatgac tggttcgggg
     4141 ggcagggaat ttacctattt tttcatcggg cagaatgaaa atcagggcat gcgtgatacg
     4201 ttgaagttta cctcaagccc tgacgaaact tctgaactca tccgggaaaa cgaagtcagg
     4261 actttaaaac tgggcctcat acgttacgtg gcgcggacac cccttgccag gtttcttaag
     4321 atcagtttca gtgaaccgct tgcccaaaca gtgacaacgg ataaatggaa cagctgggtt
     4381 tttaagacct ctgtttccgg ttatgcatca ggcgagaaat cctacaggtc atcttacctc
     4441 aacggcagct tctctgccaa ccgggtaaca gaaaagctga agtcaagtgt gtccctgagg
     4501 tacagttact cttcagataa atatgaagtt gatgatgagc tgattacgag tgaatataat
     4561 tcaaagtcac tcagcggact gttggtgggg agtcttactg atcactggtc agtgggaggc
     4621 agtatgtatg ccggtgcctc aaggtacaat aattcagacc tttctttcaa cattcagccc
     4681 gggattgagt acaacgtgtt tccatactcg gaatcgacgc gccggcaatt gcggatcact
     4741 tacaaattgg gcctcggtta tgaaaactat attgatacaa ctatctacaa caaggtaaag
     4801 gaaaaccttt tctcacaata ccttactgta aactatgagg ttatccagaa atggggatct
     4861 gctaatttgt ctctcgggta cagcaactac cttcatgact ggtctaagaa caacatttca
     4921 ctgaatgcct tccttgatct gagaatagcg aagggccttt cactgaattt tggcggaggc
     4981 ggagcaattg ttcatgatca gctgggtctt gtaaaaggag gtgcaaccca gcaggaggtg
     5041 ctgctccggt taagggaact tgaaacgcaa tttgagtatt ttacaagctt tgggctgact
     5101 tataccttcg gatcaatcta caataatgtt gtcaatcccc gcttcggaaa ctccggtggt
     5161 ggaggaatga caatcataat gaactgatgc actcagttct ggctgaaagg ctgacagaaa
     5221 tcccggaaca ttagcccttt tcaggcctta tgctcaggct taccgatggc atctgcctct
     5281 gactcccggt tcattagccc ttgtcaggcc ttatgcaccg gtttgcccac tgcatctgct
     5341 cctgcctcca ggaacacctc cgagattgtc gggtgtgcat gcattgtgtg agcgacatcc
     5401 cagacggtgc tttcaacggc catgtaggct gctgcctcgc tgatcaggtc ggtggcattg
     5461 tgcgatatga tctgtacacc cagaacctcc ccgtatttcg gttcggaaag gatgcgaagt
     5521 attccttcag gctggccctc tgtaagagcc ttgcc
//
LOCUS       DAOPXU010000148         5463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_18773_length_5463_cov_15.647744, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000148 DAOPXU010000000
VERSION     DAOPXU010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18773_length_5463_cov_15.647744"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1680)
                     /locus_tag="PKK03_11335"
     CDS             complement(<1..1680)
                     /locus_tag="PKK03_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85033.1"
                     /translation="MNSRGRTCKIFFLLAAFWLFYLAADAATYYSRQSGAWNSGFTWS
                     TSGHTGGAAASFPAAGDIVYIGNHTVTVAVNATITQVFFNRDYQPGNLVINSGITLNV
                     SGSVTVVSDDDANISHTLSGAGTLNCSSLNVGTNIDPGRNQTTSFTTDLYSLNISGNV
                     LIYSNRAGWWTSYYNNSSIVVNSGNVRVGGLIETDNENSNNTSSFSLGNGSPYLFVGG
                     ATSFDPDATGTSTIILNGAGATVDYFRSGIQTVLNTAYTNLTLSGSGVKTIPAGVTVS
                     GMLSMEGTATVSGSAPSYGTASAIRYRGSAAQTTGIELTPLFSGDGGLIIDNAAGVNL
                     GSSVTLASGLNLVAGNFNVGGNTLTLNGPAITRASGFLSTTAASSLVFGGSSPGIYIP
                     ASVITLNSLTINNANGVSLNGNLNLAAGGVLSLSAGILYAENHTISVTNTSASAVTGS
                     ESSFINVTSGGLQRTLPAGLAGTGSNYLFPIGESGTYKAINLVDVNTGATGPVLKASV
                     SPTGALNGDNTTIISVDPAYWTLQNINGGNFTRARIELYEGGLDASKTIGMA"
     gene            complement(1720..2565)
                     /locus_tag="PKK03_11340"
     CDS             complement(1720..2565)
                     /locus_tag="PKK03_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85034.1"
                     /translation="MRKLPVRHLTMLLLISLFLSPGIIKAQGDLLITPRRVVFEGNKR
                     SIDISLANIGKDTATYNISLVQIRMTEDGGFETITEPDEGQSFASPYIRFFPRTVTLK
                     PDEAQTVKIQAVRTGDLAPGEYRSHLYFRATPKEKPLGEESPVQQEQATISIKLVPVF
                     GITIPVIIRVGTPSVNVTLSDLALTFENDTLPRLSFVFNRTGDYSVYGDIEVDHVAPG
                     GTVTTVGLAKGVAVYTPNTTRKFSFNLMGGGAVDFHSGRLVVTFSAPSDVRPEKYAEA
                     IIDLH"
     gene            complement(2596..3129)
                     /locus_tag="PKK03_11345"
     CDS             complement(2596..3129)
                     /locus_tag="PKK03_11345"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HNX85035.1"
                     /translation="MKRLIPGTISLFLLLLVSAYVAEAQPGVSSTVTGHITAEVISTL
                     SAVETSQLSFGKFSPGPQGGSLILNPENTISVVGSVWPGSGSHSAASFYVTGDPGVSY
                     TVSLPVSPVTITHVSSPRTMTVEDWKSVPLAEPGAGLLENGSQVVYVGATLKVGTLND
                     NPVGVYTGTYAITFEFN"
     gene            complement(3159..3665)
                     /locus_tag="PKK03_11350"
     CDS             complement(3159..3665)
                     /locus_tag="PKK03_11350"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HNX85036.1"
                     /translation="MKKILAFSIILFAITAGTFAQNTDTEPTIATATIVGPLTLTKVN
                     DMDFGTIAVSAAVGSVVLATDNTRTASGGASLVPPANGLAASFTVAGQASETFAITLP
                     ADGTVTLTGPGVAMPVSTFNHNAGATPALNGTGTAAFTVGATLTTGASQASGAYSSAN
                     FPVTVSYN"
     gene            complement(3852..5255)
                     /locus_tag="PKK03_11355"
     CDS             complement(3852..5255)
                     /locus_tag="PKK03_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNX85037.1"
                     /translation="MSGFYGSVSERSCIDDVFYGTDYHSHLGTKRAGLVFYDREIGFR
                     RRIHNLENEYFRNKFEAELKDFRGNSGMGVISDTDPQPILFNSHLGPFALMTVSRIVN
                     INELADYFLARKKHFTENFMGNINPTEMVANIICEGDSYVEGITNVFDKIQGSCSLLL
                     LTPDGIIAARDKLGRTPVVIGRKEGARAVVSETCSFPNTGFETEYYLGPGEIVHIRAN
                     GFTRLRAPGKRMQICSFLWVYYGFPTSEYEGRNVEEVRYGCGAALARRDPVKADFVSG
                     VPDSGIGHAFGYANEKQLPYMSSFSKYTPTWPRSFMPQRQVQRELVAKMKLIPNKKLT
                     RGKKIVFLDDSIVRGTQLRDNTRDLREAGAGELHMRIACPPLLYPCPFLNFSQSRSTL
                     ELAARKAIVRLEGEEKNVEEYLDPDSEKYEMMVKEIASMLGMDSLMFQRLDDLVKAIG
                     LPREKLCTHCWDGSSSF"
BASE COUNT         1389 a         1370 c         1314 g         1390 t
ORIGIN      
        1 tgccatccca atggttttgg aagcatcaag accgccttcg taaagttcaa ttcttgcccg
       61 tgtgaaattt cctccgttta tattctgcag ggtccagtaa gccggatcca ccgaaataat
      121 tgtagtgttg tcaccattta gcgctccggt cgggctgacc gaggctttca gcaccggacc
      181 ggtggctccc gtattgacat caacaagatt aatggcctta tatgttccgc tctcccctat
      241 cgggaacaga taattgctgc cggtgcctgc aaggcctgcc ggcagtgttc tctgcaatcc
      301 tccggatgtc acattgatga aggaggactc ggaaccggtc acggccgatg ccgaggtgtt
      361 tgtaactgaa atagtatgat tctctgcata aagtattccg gctgaaagag acagcacgcc
      421 ccccgctgcc aggtttaggt tgccattgag agatacgccg tttgcattgt tgattgtcag
      481 gctattcagc gttattacgc ttgccgggat atatattccc ggggaagaac cgccgaaaac
      541 cagtgaagaa gctgcggtgg ttgagagaaa tcccgatgcg cgggttatgg caggtccgtt
      601 aagtgtaagc gtatttccgc caacattgaa atttcccgcg acaaggttaa gacctgacgc
      661 aagtgttact gatgatccaa gattaacccc ggcagcattg tcaataataa gacctccgtc
      721 accgctgaag aggggggtta gctcaatccc cgtggtttgt gctgcggagc ccctgtaacg
      781 tatggctgat gctgtgccat aagagggtgc cgatcctgaa acagtagcgg taccctccat
      841 tgagagcatg cctgaaaccg taacccctgc cgggattgtt tttacaccac tgccggacaa
      901 agtaaggttc gtataagcag tatttaagac agtctggatt ccgctgcgaa aataatcaac
      961 cgttgctccg gcgccattga ggattattgt gctggtgcca gtagcatccg gatcaaaaga
     1021 tgttgcccct cctacaaaaa ggtaaggtga accattacca agggagaagg aggatgtgtt
     1081 gttactgttt tcattatctg tttctatcag accccccacc ctgacattgc cactgttaac
     1141 gactattgaa gagttgttgt aatatgaggt ccaccacccg gcccggtttg aataaatcaa
     1201 cacgttgcct gaaatattca gggaataaag atctgttgta aaagaagttg tctgattcct
     1261 gcccggatct atgttggtcc cgacattcag agaagagcag ttcagggtac ctgcgcccga
     1321 cagagtatgt gagatatttg catcatcatc actgacaaca gtgacagatc cggagacatt
     1381 aagggtaatt ccgctattga taacaaggtt tccgggttga taatcccggt tgaaaaagac
     1441 ctgggttatt gttgcattta cagccacggt aacagtgtga ttaccgatat atacaatatc
     1501 tccggcagcc ggaaaagagg cagcggcacc acctgtatga cccgatgttg accatgtaaa
     1561 cccggaattc caggcaccgc tctgtctgga ataataggtt gctgcgtcgg cagccaggta
     1621 aaaaagccaa aaggccgcca acaggaagaa aatcttgcag gtccggcctc ttgagttcat
     1681 atcatctgtt aatttagtat caccgctgtc cggggttgat cagtgcaagt caatgattgc
     1741 ctcagcatac ttctccggcc tgacatcgga aggtgcagag aaggtcacaa ccagcctgcc
     1801 gctgtgaaag tctacagccc ctccgcccat caggttaaat gaaaattttc tcgtggtatt
     1861 cggggtataa accgcaactc cctttgcaag cccgacagtc gtaactgttc cgcccggagc
     1921 aacgtgatcg acttcaatat ctccatatac cgaataatcg ccggttctgt tgaaaacaaa
     1981 tgacaacctg ggcagggtat cattttcaaa ggtaagggcg agatctgaaa gcgtgacatt
     2041 gactgaagga gttccgaccc ttattattac aggtatggtg attccgaata caggtacaag
     2101 cttgatcgaa atcgtagcct gttcctgttg taccggactc tcctcaccca atggtttttc
     2161 ctttggggtt gccctgaaat aaaggtgtga cctgtactcg ccgggagcca gatcgcctgt
     2221 ccttactgcc tggatcttta cagtctgtgc ctcatccggt ttcagagtga ctgtcctcgg
     2281 gaaaaaccgg atataagggg aggcaaaact ttgcccttca tcaggttctg taatggtctc
     2341 gaaacctccg tcttcagtca tcctgatctg aacaagggag atattatacg tagccgtatc
     2401 tttcccgatg ttagcaagac tgatgtctat tgaccgcttg tttccctcaa ataccaccct
     2461 cctcggtgtg atgaggagat ctccctgggc cttgattatg cctggtgaca aaaagaggct
     2521 tattaacaga agcattgtca gatgccgtac gggtaatttt ctcatcacat tttgttttta
     2581 tggttatctc cggcgtcagt tgaattcaaa ggtaatggca taggtcccgg tgtaaacacc
     2641 aacgggatta tcattcaatg tacccacctt cagggtggca ccgacataaa caacctggga
     2701 tccgttttca agtaatccgg cccccggctc tgccagtgga acagatttcc aatcctcaac
     2761 agtcatggtt ctggggctgc tgacatgagt tatggtcacc ggcgacacgg gcagggaaac
     2821 cgtataggaa acccccgggt caccggtgac atagaagctg gcagcgctgt gtgacccgct
     2881 gccgggccag acactgccaa ccactgaaat tgtattttca ggatttaata tgaggcttcc
     2941 gccctgtggt ccgggggaaa acttcccgaa actgagttgc gaagtctcca ccgctgacag
     3001 tgttgagata acctctgccg taatgtggcc tgtcactgtt gaagacacgc ccggctgggc
     3061 ttccgccaca tatgcggaaa ccaacaatag cagaaaaagg ctgattgttc caggaattag
     3121 ccgtttcata gcaactgctg ccggttgtcc cttatctgtt aattatagct gacggtaaca
     3181 gggaagtttg cggaggaata ggctccggat gcctggcttg ctcctgttgt aagtgttgca
     3241 ccgacagtaa acgctgctgt gccggtgcca ttgagagcag gagttgctcc ggcgttgtga
     3301 ttaaatgtgc ttaccggcat tgccactccg gggccggtta atgtcaccgt tccgtcagcc
     3361 ggtaatgtaa tggcaaatgt ttccgatgcc tggccggcaa cagtgaatga agctgcaaga
     3421 ccattggccg gagggacaag agacgctcca cccgaagccg tcctggtatt gtcagtagcc
     3481 agcactacag aaccgacggc tgcagagact gcaatagtcc cgaagtccat gtcattgacc
     3541 ttggtaagag ttaagggccc tacaattgtg gccgttgcaa ttgtcggctc agtatctgtg
     3601 ttctgagcaa aagttcccgc tgtaattgca aacagaataa tcgagaatgc gaggattttt
     3661 ttcatctttc cttaagtatt aatggttatc tactaattta aatgtggcct agtccgcttt
     3721 gaccatttat acaaaacatc atgcactggg gttacatggc attttgtgaa gtgtaaaatt
     3781 aaacggacta aatggcctaa tattttgacg agcgtcaatt tattaacaac tttcggccgt
     3841 tttcaacacg ttcagaagct gctgctgccg tcccagcaat gggtacagag cttttcgcgg
     3901 ggaagaccga tggcctttac cagatcatca agtcgttgaa acataagtga atccattccc
     3961 agcattgagg ctatctcctt taccatcatt tcatacttct ctgaatcagg atcaagatat
     4021 tcctctacat tcttttcctc tccctcaagc ctgacaatgg ccttgcgcgc agcaagctcc
     4081 agggttgaac gcgactgtga aaaattcaga aacgggcatg gatagagtag cggcggacat
     4141 gcaatgcgca tatgcagctc gccggcgcca gcctcccgca gatcccgcgt gttgtccctc
     4201 agctgtgtcc cccgtacaat gctgtcatca agaaatacta tcttcttgcc cctggtaagc
     4261 tttttgttcg gaatgagctt cattttagcc acaagctcgc gctgcacctg cctctgcggc
     4321 atgaaactcc tcggccaggt gggagtgtac ttggagaagg agctcatata gggtagctgc
     4381 ttctcattgg cataaccaaa cgcatggcca atccccgagt cgggaactcc agacacgaag
     4441 tcagccttca ccgggtcacg cctggcaagc gcagcgccgc aaccgtaacg cacctcctct
     4501 acattcctgc cttcatactc agaagtgggg aatccgtaat aaacccatag gaaagaacag
     4561 atctgcatcc gctttccggg tgccctgagc ctcgtaaacc cgttcgcacg gatgtgcact
     4621 atctcccccg gcccgagata atattcggtt tcgaaaccgg tattcgggaa agaacaggtc
     4681 tccgatacca cagcacgggc cccctccttc ctgccaatta ccacgggcgt tcttccgagc
     4741 ttgtcacgcg ctgcaatgat gccgtcagga gtcagcagca gcagtgaaca tgatccctgt
     4801 atcttgtcaa atacatttgt gataccttcg acataggaat ctccttcaca gataatattg
     4861 gcaaccatct cggtggggtt gatgttgccc atgaagtttt cagtgaaatg cttcttgcgc
     4921 gccagaaaat aatcagcgag ctcatttatg ttcacaatcc gcgagacggt catcagggca
     4981 aaagggccaa gatgcgagtt gaacagtatc ggctgcggat cagtgtcact gatcaccccc
     5041 atcccgctgt tccctctgaa atccttcagc tccgcttcga acttattccg gaaatactca
     5101 ttttccaggt tgtggatcct cctcctgaat ccgatctccc tgtcgtaaaa taccaatccc
     5161 gccctcttgg taccgaggtg ggaatgatag tccgtaccgt aaaacacatc atcaatacaa
     5221 ctcctttcgg agaccgaacc gtaaaatccg ctcatgagaa aatataagtg tgattttttt
     5281 tataaattgt aaggcgaaga taatcaaaca ggagaaaaat ttgttgtact ataataattg
     5341 ttaattttgc accggattaa aatacaaaca aatggaaaat caggaaaaat atcaggctga
     5401 acacgttgag cataagaaag gcgctccgat tgtcatgatt gtgtcaacca taatcctgac
     5461 cct
//
LOCUS       DAOPXU010000149         5382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_19183_length_5382_cov_12.268444, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000149 DAOPXU010000000
VERSION     DAOPXU010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19183_length_5382_cov_12.268444"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            912..2363
                     /gene="cls"
                     /locus_tag="PKK03_11360"
     CDS             912..2363
                     /gene="cls"
                     /locus_tag="PKK03_11360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HNX85038.1"
                     /translation="MRWSLLLEISYGIILLAACIRIIYDTRSSTKTLAYLLLAVFVPV
                     AGMIFYFLLGVNYRKHQIYSKKLIMDEKASRALNEMIISVTEKNLLENEDVINDTRGI
                     VRILLYDNLSPLTAGNRIALLINGEEKFPEVIKAIESARDHIHLEYYIYENDTIGNKI
                     KDLLIRKAREGVKVRLIYDDFGSRSIRKQLVKELRNGGVEAFPFNRIRLPFFANRINY
                     RNHRKIIVVDGTTGFTGGINISDRYINDPRMDNRYFWRDTHIRIDGPGIIFLQNIFLC
                     DWNFCSGQQIEPEKKHFNTSRQPGGNIYVQIAASGPDSPNSTIMLSILKAVSIAKEEI
                     LITTPYFIPGGSIIDSLKMALLGGVSVKLLVPGISDSRIVNAAARSYYGELLGAGAEI
                     FLYRKGFVHSKIMVIDGSFAVVGSANMNHRSFNLDFEVNANIYSAEFAGRMKRMFLAD
                     IKDAEKIEYQQWQDRPLLVRLVERTARLLSPLL"
     gene            2473..3171
                     /locus_tag="PKK03_11365"
     CDS             2473..3171
                     /locus_tag="PKK03_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HNX85039.1"
                     /translation="MDLKRASYLFEGIKGQILPENLIDTIRSFIVAENLYSSAAREIA
                     TKLENLNSEFNSTHERNPIHLIQTRVKTPASILEKLKKRGFELSVDSARNNLTDIAGV
                     RVICSYINDIYMISEFLLSQFDIQLVRTTDYIKNPKPNGYRSLHHIVKVPVYLSDRME
                     LVNVEIQIRTIAMDFWASLEHELAYKLEREKSGEAFAELKACAAEIADIDRRMQNLYN
                     ISSDHQVLPEEHLQ"
     gene            3585..4133
                     /locus_tag="PKK03_11370"
     CDS             3585..4133
                     /locus_tag="PKK03_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85040.1"
                     /translation="MDRKEFFRKTCLAGVCSCGFGALALKAGDIARADQDNISPVPQQ
                     WLINLLENLNRNVDQPELRKIIKMSAGVHYDQLKIDDLLSGYIGKLKEFVVFLEKEWV
                     WKVNYDEATGVITADENKSYCVCPVLKGTPAGSPVICYCSEGFAERMFSKVAGVQVSA
                     QVISSVRRGDASCIYRIELPSR"
     gene            complement(4130..5173)
                     /locus_tag="PKK03_11375"
     CDS             complement(4130..5173)
                     /locus_tag="PKK03_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006848275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HNX85041.1"
                     /translation="MLTPTIFFIIFGVLIVSVLLIDLLVVGRGYHTVSLKESLIWTAV
                     WVSLAAGFYFFIRFYGQIIHGIGDWESLKAYVSHYLPALHLPDDFGAALDLYRKELAL
                     NYITGYLLEYTLSIDNVFVILMILTGFSVAPQNYKPVLFWGVLGAIVLRFIFIFAGSA
                     LIHRFSWLLLIFGAFLIYSGIHMFVTRNRNEKTDIQKHWLVRFLSGRINIYPDYIENR
                     FWKRIDGRFFFTPLFIVLIMIEFTDLIFAMDSIPAIFSITTDPYIVFFSNVFAIIGLR
                     SLFFLLAKLVNLFRYLKVGISFLLAFVGFKLLFHTWLDNIGYQTTYSLYIIAGTLLIS
                     IVASVAFPEKGKV"
     gene            complement(5303..5376)
                     /locus_tag="PKK03_11380"
     tRNA            complement(5303..5376)
                     /locus_tag="PKK03_11380"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5340..5342),aa:Arg,seq:acg)
BASE COUNT         1514 a         1159 c         1280 g         1429 t
ORIGIN      
        1 gcaatgaatt ccagggtaca ggtagtgtca aaagctgtcg agaagcttga atcagttatg
       61 gttgtgcccg atatttcaaa gatacattct gaaagcaatt cacaaccaca ctcttcttcg
      121 gtccagtgat gtcttagttg tgcccgatat ttcaaagata cattctgaaa gcaattcaca
      181 acctgtctct tccaggtagc ggaagaattg acgctgtgtc cggtatttca aagatacatt
      241 ctgaaagcaa ttcacaacgc gttcggactt actactatca ttatgtacct cggcatattc
      301 cttatcacta gagaaaatca cctgactctt cgtggtagtg gtttttatgg tatcattgag
      361 gtgtgtgccc ttagtgtctg tctggtggct tatcaggatt ccccggtcgc tatctttctt
      421 ccgccacttg gagtatgcgg cagagatctt tccgcccatc gttttttttt cggtcccttt
      481 cttttcctcc atttgaccag gttccggtca gtaaatccct agttatccca gctctcttca
      541 aagtgtcgtt gtccctccac agatactact tcaggaagga catccctgat tttgtttact
      601 acggccctgc ttatcctctg cagatctcct ggcagtattg cgattatttt gccactttac
      661 ttgactttgg tacataaatt gccttacttt tgttgtctgg agggctcctt cgggatccca
      721 ccaaccaaag attgcctgac gtaagccagg ctttcttatt ttaagaaagt cgtgcccgaa
      781 attccaaaga tacattctga aaccaattca caacggatag ctcccttgcc gcttcaggcc
      841 aacggatttt gcagtatcac ctattcatgt taattttagc ggatagtaca ctttgtgaca
      901 atcattgaca aatgcggtgg agtttacttc ttgagatcag ttacgggatc attctgcttg
      961 cagcctgtat ccggattatt tatgatacac gcagttcaac caagaccctt gcatatctcc
     1021 tgctggctgt gtttgtgccc gtcgcaggca tgatcttcta cttcctgtta ggagtgaatt
     1081 atcgcaaaca ccagatatac agcaaaaaac tcattatgga tgaaaaagca tccagggccc
     1141 tgaatgagat gataatttca gttactgaaa agaaccttct ggagaatgaa gatgtgatca
     1201 atgataccag gggaatagta aggatactgt tgtacgataa ccttagtccg cttacagcag
     1261 gaaacaggat agctcttctc atcaatggtg aggaaaaatt tcccgaagtg atcaaagcca
     1321 ttgaatcggc cagggaccat attcacctcg agtattatat atatgaaaat gacacgatcg
     1381 ggaacaaaat caaggattta ctcatcagaa aagcccgcga gggtgtaaag gtcaggctga
     1441 tatacgatga ctttggaagc cggtcgataa ggaaacagct ggtgaaggaa ctgaggaacg
     1501 gaggagtcga ggcattcccc tttaaccgca taagactgcc gttttttgcc aacaggatca
     1561 attacaggaa tcacaggaaa attattgtcg tagacggcac aacaggattt acgggaggga
     1621 tcaatataag cgacaggtat ataaacgacc cccgaatgga taacaggtac ttctggcgag
     1681 atacccatat ccgcatagac ggacccggaa ttattttcct gcagaacatt ttcctgtgcg
     1741 attggaactt ctgttccggt cagcagatag agccggagaa aaaacacttt aacacttccc
     1801 gtcagcccgg tggaaatatt tatgtacaga ttgcggcaag cggtcctgac tctcccaact
     1861 caaccataat gctatcgatt ctcaaggctg tttccatagc gaaggaggag attctcatca
     1921 ctacccctta ttttatcccg ggaggttcaa taatagactc cctcaaaatg gccctgctgg
     1981 ggggtgtatc ggtgaagctg ctggtccccg ggatatccga ctcgcgaata gtaaatgcag
     2041 cagcaaggtc atactacggg gaactgcttg gtgccggagc tgagatattc ctgtaccgga
     2101 agggatttgt acattcgaaa ataatggtaa tcgacggatc ttttgcagtt gtcggatctg
     2161 caaatatgaa ccatcgcagt ttcaatctcg atttcgaggt gaatgccaat atctattcgg
     2221 cagaattcgc cgggcggatg aaacgcatgt tccttgccga tataaaggat gcagagaaaa
     2281 ttgaatatca gcagtggcag gaccgccccc tgcttgtcag attggtggag cggacggcca
     2341 gactactgtc tcctcttttg taggcataca tggatttgtt cttccgtttt ttttctccac
     2401 ctttacgtga ctttattatt actgaagtca gcccggatag aaatttacat ggaggataaa
     2461 gagatgaaca gaatggacct taaaagggca tcatatcttt ttgaagggat caagggacag
     2521 atattgccgg agaatctgat agacaccata aggtcattta ttgtagctga aaacctatat
     2581 tcatcagccg cccgggagat cgccacaaag cttgagaatc tgaatagcga gttcaatagc
     2641 acacatgaac ggaatccgat tcatctgatc cagacgaggg taaagactcc ggcaagtatt
     2701 ttggaaaagc ttaagaagag aggctttgaa ctcagtgtgg attcggccag gaacaacctc
     2761 acggatattg ccggtgtaag ggttatttgt tcatatatca atgacatata tatgatttcc
     2821 gagtttctgt tgtcgcagtt tgatattcag ctcgtacgca ccaccgatta tattaagaat
     2881 ccgaaaccga acgggtaccg tagtcttcat catatagtca aagttccggt ttacctgtct
     2941 gacaggatgg aacttgtgaa tgtcgagata cagatcagga ccattgcgat ggacttctgg
     3001 gcatctctgg agcatgagct ggcgtataag cttgaaagag aaaagtcggg ggaggcgttt
     3061 gccgagctta aggcctgtgc tgcggagatt gcggatattg accgccggat gcagaacctg
     3121 tacaatattt cttctgatca tcaggtttta cccgaagagc atctgcagta ataatgctat
     3181 tgcctgaaga tcgtttgtgt ttcaatatcg caagggtctg tgccggtaaa gactggctgt
     3241 tctgaatcgt ctggccaaca acccttcatg ctgatcatta ataatctgca tggactgtgg
     3301 gtcttattgc gatcctgtta tgcgttcatt accagaggat tgagtatgaa tattttaagc
     3361 agtcctgtta aagcgacaaa atgcaaggtg ttataagatg atttttaacc agctagttta
     3421 tagtccctga tcagaagtcc gggggaaaga tttaatctct cagtcagatt tcgaatcatt
     3481 tccacagtca atttgcgctt ccggttcagg atctcggaaa tcctgttcta aaggcaatct
     3541 tcagaagact tttatgatta gctttaaaca ttgtttcaga ttctatggac aggaaggaat
     3601 ttttcaggaa aacatgtctt gccggagttt gcagttgcgg attcggggcg ttggcattaa
     3661 aagcgggcga tatcgccagg gctgaccagg ataatataag tccggtgcca cagcagtggc
     3721 tgatcaactt gcttgaaaac ctgaaccgga atgttgacca gccagagttg aggaagataa
     3781 tcaaaatgtc tgccggtgtt cactacgatc agttgaaaat tgatgatctg ctttcaggtt
     3841 acatcggaaa gctaaaggaa tttgtagtct tccttgagaa ggagtgggtc tggaaagtga
     3901 actatgacga ggctaccgga gtcatcacag ctgatgaaaa taaaagttat tgtgtatgcc
     3961 cggtgctgaa aggcacgccc gcaggttcgc cagttatctg ctattgttct gaagggttcg
     4021 ccgagaggat gttctcaaag gttgccggag tacaggtttc agcacaggta atctcctcgg
     4081 tgagaagggg agatgcgagc tgcatataca gaatcgaact gccctcccgc taaaccttgc
     4141 ccttttccgg gaaagccacc gaggctacaa tgctaatcag tagggtgcct gcaataatgt
     4201 acaacgaata ggttgtctgg tagccaatgt tgtcaagcca ggtgtgaaac agcagcttga
     4261 acccgacaaa agcaagcaaa aatgaaattc caaccttaag ataacgaaac agattcacaa
     4321 gcttcgccag caggaagaat agtgaacgga gaccgattat tgcgaagacg ttggagaaaa
     4381 acacgatata cgggtcagtg gtaattgaaa atattgcggg gattgagtcc attgcaaaga
     4441 tcaggtccgt gaactcaatc ataatcagga caatgaacag aggtgtgaag aagaacctgc
     4501 cgtctattct tttccagaag cgattctcaa tataatcggg ataaatgttg atacgtccgg
     4561 aaagaaacct tacgagccag tgtttctgaa tgtcggtttt ttcattgcgg ttccttgtga
     4621 cgaacatatg tatgcccgaa tagatcagga atgcaccgaa gatcagcagg agccagctga
     4681 acctgtgtat cagggctgat ccggcgaaga tgaaaataaa ccttaggaca attgcaccca
     4741 gcacccccca gaacaggacc ggcttgtagt tctgaggcgc caccgagaaa ccggtcagta
     4801 tcatcaggat cacgaagacg ttgtcaattg acagggtata ttcaagaagg taaccggtaa
     4861 tataattgag ggcgagctct tttcggtaca gatccagcgc agctccgaaa tcgtccggca
     4921 gatgaagcgc cggcagatag tgtgatacat aggctttcag cgattcccag tcgcctattc
     4981 cgtgaattat ctgaccgtaa aaccggatga aaaaatagaa tcctgctgcc agactcaccc
     5041 agacagctgt ccagataagt gattccttca gcgagactgt atggtaaccg cggcccacga
     5101 cgagaaggtc aatcagcagg accgagacta tcaatactcc gaagataatg aagaagattg
     5161 ttggcgtcag cacttgtgtc aggtttaaat gatgcgcaaa aataacagat aaggggataa
     5221 atcgttcagc agtacgactc tccgaagcaa gattcggagg ccctttcaaa aaaaaatcct
     5281 gcttgcgcag gatgatttct tgtgatcccg gcgggagtca aacccacaac cttctgatcc
     5341 gtagtcagat gctctattca gttaagctac gggacctttt gc
//
LOCUS       DAOPXU010000150         5350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_19358_length_5350_cov_11.597923, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000150 DAOPXU010000000
VERSION     DAOPXU010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19358_length_5350_cov_11.597923"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PKK03_11385"
     CDS             complement(<1..434)
                     /locus_tag="PKK03_11385"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNX85042.1"
                     /translation="MKELVAVTLMILFSFSSAAQEPRYPAALIAKGARVQKAGSGYAF
                     TEGPSVARDGRVFFTDQPGDRIYAWDEKSGITIFKKNCGRANGTRFDREGNLLACADQ
                     HNTIIKITPHGELITVYDKGFGGVHLNGPNDLWQDMKGGIYFT"
     gene            773..1813
                     /locus_tag="PKK03_11390"
     CDS             773..1813
                     /locus_tag="PKK03_11390"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNX85043.1"
                     /translation="MERRSIGKTGISIPPVIFGTSALGNLYSALSDETKTEIIKECLL
                     NVPGPVAFDTAGKYGAGLALEKLGDILCRLNAEPEDVIISNKLGWLRTPLLTAEPAFE
                     KGIWFGIKNDARQNISYDGILECWEQGNRLLGGKYKPGLVSVHDPDEFLAASRDQRDF
                     NIHLNNILDAYKALDNLRSEGEVKAVGIGAKNWKTIQLIAGETRLDWVMFANSMTIYR
                     HPEDLLVFMGKLHSMGIAIINSAVFHAGFLTGGDYFDYVRIKPDSEENKAKFRWREDF
                     FAVCRKYNIVPADACVQFAITPPGVVSISLNTSDPGRVKSNVESVERQIPVEFWREMI
                     SNGLIDGDYPYL"
     gene            1847..2905
                     /locus_tag="PKK03_11395"
     CDS             1847..2905
                     /locus_tag="PKK03_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNX85044.1"
                     /translation="MNKMKAAVLTEYRKVEWKEVDIPSVSGNEVLIKVSYASICGSDQ
                     HVFLGEFHPRTSVPLIQGHEFAGTIVQKGPAVRNFKEGDRVAVDPIIWCGHCPACKRH
                     HYPACSTLKLVGIDLDGGFSEFMKVPESMLYKVTEKISDMHAALVEVLSIGFHACKRA
                     GVSEGDRIAIWGAGKVGQSILQAVRTKTRNTVVMVDILDERLAIAQKAYNDIHIINAA
                     NTNAVEKIRELTGGEGVDIAFEAVGHAKELPGVINPVRGCVQAIRGAGKVCVLGLSSE
                     PSPVVMKELILKEGIIMTSRVSHGEFAETIENMSRDTLKPEAVVTDIMHPSATQAAFE
                     MLEKEPQKHLKIMLKFSE"
     gene            2902..3738
                     /locus_tag="PKK03_11400"
     CDS             2902..3738
                     /locus_tag="PKK03_11400"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNX85045.1"
                     /translation="MKIDAHQHFWIYNSSEYGWIGHNMDMLKRNYTPEDLYNELIKAG
                     YSGSVAVQARQSVEETRWLLELAGKHDFIRGVVGWADLSGEDDLRRQLDDFCRNEKLV
                     GLRHVIHDEPADSFMLRDDFLKGISILKEYDLTFDLLLFPKHLPVAQLLVSLFPEQKF
                     VLDHISKPLIRNKVVSPWREDITRLAQNENVWCKLSGMVTEAESKSCDHEDFRPYLDV
                     VFEAFGTERLMIGSDWPVCRLAGEYQDVVGIVEEYISGMSDDLRTGITGENCINFYGL
                     ER"
     gene            4123..>5350
                     /locus_tag="PKK03_11405"
     CDS             4123..>5350
                     /locus_tag="PKK03_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HNX85046.1"
                     /translation="METPTMGVIAYKGIPFAAPPVGDLRWKEPQPVVPWEGVKVADTY
                     GDAASQVTWDPESFYGKEWRASGSVPFSEDCLYLNIWTPAAGKTGKKLPVAMWIHGGG
                     YREGFSYEPEMDGGEAWASRGVILVQVTYRLGVMGFFSHPLLSAESPHGVSGNYGLMD
                     QIAALKWIHNNIEQFGGDPGNITIFGQSAGAGSVQSLCASPLSRDLISNAIIMSGGGL
                     SDLRPGIMMDTAQVSGKNLMDHFEKTTLEDMRALSFDELTKMSGEYAAATNRRTWWSP
                     VVDNYVLTGTFTTVARAGEIADIPYIIGFTANDLNDMTKPVTDFCALRAEKSDKPAYA
                     YLFARPLPGDDSGAFHSADLWYVFNSFKHSWRPFTAGDNELSNKIVDYWTNFAKHGNP
                     NGLGEQTWTPYTSQLPK"
BASE COUNT         1447 a         1162 c         1348 g         1393 t
ORIGIN      
        1 gtgaagtaga taccgccctt catgtcctgc cagagatcat tgggtccgtt caaatgaact
       61 cctccgaaac ctttgtcata gacggtaatc aattccccat ggggagtgat tttgataatg
      121 gtgttatgct gatcagcaca tgccagcagg ttaccctctc gatcgaacct tgtgccgttt
      181 gcccgcccgc aattcttttt aaagattgtt attcctgatt tttcgtccca ggcatagatc
      241 ctgtcgccgg gctgatcagt aaagaagacc cttccgtcac gggctacaga gggcccttca
      301 gtaaaggcat accctgaacc ggctttttga acccgtgctc cttttgcgat taatgctgca
      361 ggatatcgcg gttcctgtgc cgctgatgaa aatgaaaaaa gaatcataag ggtgaccgca
      421 accaattctt tcattgcttg aatctttact taattaacac taagatggtt gattatggtt
      481 tacattgagt gaaaacatgg aataactggt tcgatcacat gccggaatct gatttatttt
      541 tgccggatta cttaaaactg acaggtgcat ggttggcata atcctgaaat attgaggata
      601 tttaacctga tatttatctg atcatggatt ttgctccggt tataaagggt cagtcaaacc
      661 cgattgccga cgggcagggt aaacagctac tccgcctggc cgacgggctg taccttatag
      721 gttgaaacgg ggtacataaa cattacgact tatcaacctt tgacaacaaa ttatggaacg
      781 ccggtcaatt ggaaaaacgg gaatcagcat acccccggta atatttggca ccagcgccct
      841 ggggaatctt tattccgctc tcagcgatga aacaaaaaca gagataataa aagagtgcct
      901 tctgaatgta ccagggccgg tagccttcga taccgccggc aagtatggtg caggattggc
      961 acttgaaaaa ctcggtgata tcctttgcag gctgaacgct gagcctgaag atgtaataat
     1021 cagcaataag cttggctggc tacgcactcc cttactgact gccgaacctg ctttcgaaaa
     1081 aggcatatgg tttggcatta aaaacgatgc ccggcagaat atctcatacg acggtatcct
     1141 ggaatgctgg gaacagggca acaggcttct cggtggaaaa tacaaacccg gactcgtttc
     1201 ggtccatgat cctgatgagt ttcttgccgc ttcgcgtgat cagcgcgact ttaatataca
     1261 ccttaataat atacttgatg cgtataaggc tctcgacaat ctcaggtcgg agggcgaggt
     1321 aaaggcagtg gggattggtg caaagaactg gaagaccata cagttgattg ccggtgaaac
     1381 ccggctagac tgggtgatgt ttgccaacag catgacaatt tacagacacc ctgaagatct
     1441 tctcgtattc atgggaaagc ttcattcaat gggtattgcc ataataaact ccgctgtgtt
     1501 tcatgccggg ttcctgaccg ggggagatta tttcgactat gttcgaatca aacccgattc
     1561 ggaggagaac aaagcaaaat tcaggtggcg ggaggatttc tttgcggttt gcagaaaata
     1621 caatatcgtt cctgcagatg cctgcgtgca gtttgccatt acaccccccg gtgttgtcag
     1681 tatttcactc aatacatctg atcccggccg ggtaaaaagc aacgttgaat cagttgaacg
     1741 ccagatcccc gtggagtttt ggcgcgagat gatcagcaat ggattgatcg atggcgatta
     1801 tccatatctt taagctgaat tgtaatctgt tataataaat cctgaaatga ataaaatgaa
     1861 agccgctgta ctcactgaat acaggaaggt tgaatggaag gaggttgata taccttctgt
     1921 ttcgggcaat gaagtcctga taaaggtaag ctacgcctct atctgcggaa gtgatcagca
     1981 tgttttcctt ggtgagtttc atccccgtac aagtgtgcct ttgattcagg gacatgagtt
     2041 tgccgggacc atcgtgcaaa aaggacccgc tgtcaggaat ttcaaggaag gcgacagggt
     2101 ggcagtcgat ccgatcatct ggtgcggtca ctgcccggca tgcaaaagac atcattatcc
     2161 agcctgctcc accctgaaac tggtggggat tgatctagat ggaggcttca gcgaattcat
     2221 gaaagtaccg gagagcatgc tttacaaagt gacagaaaag atttccgata tgcatgccgc
     2281 actcgtcgag gtcttatcca taggatttca tgcctgcaaa agggccggag tatctgaggg
     2341 tgacaggata gccatatggg gtgcaggcaa ggtaggacag agcatcctgc aggccgtcag
     2401 aacaaagacc aggaacacgg ttgtaatggt cgatatactt gatgaacgtc ttgccattgc
     2461 ccaaaaagca tataatgata ttcacattat aaacgcagca aatacgaatg ccgttgaaaa
     2521 gataagggaa cttaccggag gagaaggagt ggatatcgct tttgaggccg tgggccatgc
     2581 aaaagagcta ccgggggtga tcaaccccgt gagaggatgt gtacaggcaa tcaggggggc
     2641 aggcaaagta tgtgtactgg gactttcatc tgaaccatct cccgtcgtga tgaaagaact
     2701 tatcctgaaa gaagggatca taatgacctc ccgggtttct cacggtgaat ttgccgaaac
     2761 tatcgaaaac atgagcaggg acacacttaa gcctgaagcg gtcgtaactg atataatgca
     2821 tccttccgca acacaggcgg catttgaaat gcttgaaaag gagcctcaga agcatctgaa
     2881 aatcatgttg aagttcagtg aatgaaaata gatgcacatc agcacttctg gatctacaac
     2941 agcagcgaat acggctggat aggccacaat atggatatgc tgaagcgcaa ctatactccg
     3001 gaagaccttt ataatgagct tatcaaagcc ggatattcgg gatcggtggc tgtccaggcc
     3061 cggcagagtg ttgaagaaac ccgatggctg ctggagctgg caggaaaaca tgatttcatc
     3121 aggggagtag ttggctgggc tgacctgtcc ggtgaagatg atttgagacg gcagctggac
     3181 gatttctgca gaaatgaaaa gctcgtcggc ctgagacatg tcattcatga cgaacctgct
     3241 gacagtttca tgcttcgcga cgatttttta aaaggtatca gtatcctgaa ggagtatgac
     3301 ctcacattcg accttctcct tttccctaag catttgcctg tcgcacagct tcttgtgtca
     3361 ctctttcccg aacagaaatt tgtcctagat cacatctcca aaccactgat aagaaataaa
     3421 gttgtcagtc cgtggcgtga agacatcacg cgcctggcgc agaatgagaa tgtctggtgc
     3481 aagttatcgg gaatggtcac ggaggctgaa tcgaagagct gtgatcatga agacttcaga
     3541 ccatatctcg atgttgtctt tgaagcattt ggcactgaaa ggcttatgat tggaagcgac
     3601 tggcccgtat gcaggctggc aggagaatat caggacgttg ttggcattgt cgaagagtat
     3661 atctcaggaa tgtcagatga tctccgtacc ggtatcacag gtgaaaattg catcaatttt
     3721 tacgggctgg aaagatgaca ggttgaacgg tcgaaaacct aaagtccggg tagtctgcat
     3781 attacgtagc agattccatt acattcccat gtgccttcat catgccggtt tatcattaac
     3841 aggaaatcag attcttttag ctcttttatc atcgactttt aaatttcagt catcatttgc
     3901 atataaaatg aacctgtctt aaaataattt ttatgagttt tacaattacc atgattcccc
     3961 ggttcctctt attatgaaca gagctaaatc aatatatcat ttaactattg taccattatg
     4021 aaacgtatat ctttctttat tctgacaatc attacgttgg catcagtctc atgcagtgat
     4081 tcaaatcccg aattgcaaat tgaaggcggc cgggtaattg ggatggagac accaacaatg
     4141 ggtgttatag cttataaagg cattcctttc gcagcccctc cggttgggga tctgcgctgg
     4201 aaggagccgc aaccggtagt gccctgggaa ggtgttaagg tggcagacac ctatggggat
     4261 gctgcttccc aggttacttg ggatcccgaa agtttttacg gcaaggaatg gagggccagt
     4321 ggctccgttc cgttcagcga ggactgtctc tatcttaata tctggactcc cgcggctggt
     4381 aaaacgggca agaaactacc tgttgccatg tggatacacg gtgggggtta tcgcgaaggc
     4441 ttctcctatg agccggagat ggatggcggt gaggcctggg catcacgtgg tgtaatcctg
     4501 gtgcaggtaa cttatcgtct tggtgttatg ggatttttct cacatcccct tttatctgca
     4561 gaaagtcctc atggtgtatc tggaaattat ggcttaatgg accagattgc agcacttaaa
     4621 tggattcata ataatatcga acagttcgga ggtgaccccg gcaatataac catctttggt
     4681 cagagcgcag gtgcaggcag cgttcagtca ttatgcgctt ctccgctgtc aagggacctc
     4741 atcagcaatg ctattataat gagcggaggc gggttgagtg acctgaggcc cggtatcatg
     4801 atggatactg cacaggtgtc cggcaagaat ctgatggacc attttgagaa gaccacgctg
     4861 gaggatatgc gtgctctttc attcgatgaa cttacaaaga tgtcaggtga gtatgctgct
     4921 gcaacaaaca ggcgtacatg gtggagtccg gttgtggaca attacgtttt aacgggtact
     4981 tttacaactg ttgcccgcgc cggagaaatt gcagatatac catatataat tggtttcacg
     5041 gccaatgacc tgaatgatat gactaaacca gttacggatt tctgtgcact tcgggcagag
     5101 aagagcgaca agcctgccta tgcctattta ttcgcacgcc ccttgccggg ggacgatagc
     5161 ggggctttcc actctgccga cctgtggtat gtcttcaatt cgtttaagca cagttggagg
     5221 ccttttacgg ccggtgataa tgagctgagc aacaagatcg tagattactg gaccaatttt
     5281 gccaaacacg gcaatccgaa cggattgggg gaacagacct ggacacctta cacatcacag
     5341 cttccgaagt
//
LOCUS       DAOPXU010000151         5219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_20036_length_5219_cov_12.368513, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000151 DAOPXU010000000
VERSION     DAOPXU010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20036_length_5219_cov_12.368513"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PKK03_11410"
     CDS             <1..756
                     /locus_tag="PKK03_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85047.1"
                     /translation="TTEVINGKTIYSGYLRNAYLQYENGRLLVRGGLIGTEQISMMEK
                     IWGYRYVTKPPIDYSGMIQAADLGVMTVVQAGEFLDLDLSVTNGRGFKDIAANGTYRL
                     SAGFTLTPAQNMLVRGFYDMMGPSGRMQRTASITAAWMGPKFNAGAEYFRQDNASMTE
                     GRNYSGISIFTRVPLAEKVKFFARYDRIASVTMDGAEDPWNLSRDGSYIYAGIDFSPV
                     KNVRISPNYNSYIPPAENSDNVGTIGLNIEAKF"
     gene            831..1895
                     /gene="pstS"
                     /locus_tag="PKK03_11415"
     CDS             831..1895
                     /gene="pstS"
                     /locus_tag="PKK03_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HNX85048.1"
                     /translation="MKKQIFILMAAMVLLLVSCGGGGSTTGKQKQITLSAAGATFPQP
                     FYNLAFKKYNEAGGPLTNYGGIGSGGGIKSLRDQIVDFAATDAYLSDAELAEMPGEVV
                     HIPTCIGAVVIAYNLPGVTDLQLTPELLEGIFLGNIKKWNDPKILAANPGLAVPDMEI
                     TVVYRSDGSGTTYIFSDYMSKVSPAWKAQLGTGKALNWPAGIGAKGNPGVSGTISQSQ
                     GSIGYIGYEYAFAQKIPMAKVMNSAGLFIEPTLESFTAAAAAEMPADTRVMLTNSPNP
                     AAYPITSFTWIIMYKELAYGKHSSEAAEAAVKTVKWLTGEEAQAIATSVHYAALPQNA
                     SLQAAELLRGITYQGLTVLR"
     gene            1900..2751
                     /gene="pstC"
                     /locus_tag="PKK03_11420"
     CDS             1900..2751
                     /gene="pstC"
                     /locus_tag="PKK03_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNX85049.1"
                     /translation="MSGDRVTKVLLFISSLTILLLSAGIVISLFGGALPAFREFGFRF
                     ITSSAWNPTAGSEEYGAMSFIAGTVITSILALIICIPMAFSTSLFIAEYYNGTRTSGI
                     LRSLVDLLAGIPSIVYGFWGFYALRPLMVATGLTDQGFGVFTASLVLAIMIVPYATSL
                     SAEIISMVPAELKEAAYSLGATRREVIFSVVIPSARSGIIASFILALGRALGETMAVT
                     MLIGNANVIPKGLFAPGNTMASVIANQFGEASGLKMSALIAIGLLLFLITALVNYLGK
                     LIIKRFA"
     gene            2748..3638
                     /gene="pstA"
                     /locus_tag="PKK03_11425"
     CDS             2748..3638
                     /gene="pstA"
                     /locus_tag="PKK03_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNX85050.1"
                     /translation="MTEMYPVLTDNISRRNRTNIRFRYLVIAGSVITVIPIFLIVGNL
                     AVKGFRQINLAFFTKPAPDTFQAMMAASSGEIIPGGIANGLTGSLLMVVLASLFAIPL
                     GLMTGIYIYENSSLKYARFIRSLTEILQGVPSIILGIIAYIWIVKNITRGYSALAGSV
                     SLAIMMLPLIIRSTEETMRMIPVSIREAAFALGTPYYKMIYKILIPVSFSGLATGVLL
                     ALSRILGETAPLMLTALGSPAISLDVTRPVSAVPLLIWDFYNDPNMVSLVWSASLFLM
                     VFVLSMNLLARSVASKHKYI"
     gene            3644..4420
                     /gene="pstB"
                     /locus_tag="PKK03_11430"
     CDS             3644..4420
                     /gene="pstB"
                     /locus_tag="PKK03_11430"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNX85051.1"
                     /translation="MNNTPQSNNVLEIRDLSIAYEPGKNAVNGVTASVKRNSITAIMG
                     PSGCGKSTLLRAMNRMHELYPNIRTKGDILLDGQSIFSMSVIELRRKVGMVFQRPNPF
                     PNMTIYENVVAGFSLNDIRLKKSQRDEIVENALRNVTLWDEVKESLYRKGTFLSGGQQ
                     QRLCVARAIAFNPEVLLLDEPTSALDPIATSKIEDLLGELKKKYTLVLVTHNMSQAAR
                     ISDDSMFMYLGELVESGRTGTLFTTPKDPRTEAYLTGKFG"
     gene            4460..5152
                     /locus_tag="PKK03_11435"
     CDS             4460..5152
                     /locus_tag="PKK03_11435"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HNX85052.1"
                     /translation="MSEKKEQALNEIKTSFGEMASLVLRQLTLLEKLMGTTEEQAFTD
                     LIDEMEKAESRIDNLEVVIGEQFTSTIVLYQPVASDVRRLVAIYRMTINLERIGDRIH
                     NLCRIIGDIRNTTEFTSTASLITNMLTSGTIMVEKALLSFTNSDPDYAIWTIKNDTVV
                     DEMNHKLLLGHLRKENIDDRTRSMVQSYIELKDMISNIERIADHATNIAEASIYSLQG
                     TDIRHTGIGKEE"
BASE COUNT         1323 a         1415 c         1344 g         1137 t
ORIGIN      
        1 accaccgagg ttatcaacgg gaaaaccatt tattccggtt acctgcgaaa cgcctacctt
       61 cagtatgaaa acggcaggct cctggtaagg ggcggactga tcggtaccga gcagatctcc
      121 atgatggaaa agatctgggg atacaggtat gttacgaagc ccccgataga ttattcaggc
      181 atgatacagg cagctgacct gggtgtgatg acagtggtgc aggcaggtga attccttgac
      241 cttgacctct cagtcacaaa cggacgcggt ttcaaggata tagccgcaaa cggaacctac
      301 aggctttcag caggattcac cctcaccccg gcacagaata tgctcgtaag gggcttctat
      361 gacatgatgg gtccttccgg caggatgcag cgtacggcca gcattaccgc agcatggatg
      421 ggtccgaagt tcaatgccgg agcagagtac ttcaggcagg ataatgcatc catgaccgaa
      481 ggacgcaatt actcaggcat cagcatcttc acaagggtgc cgctggcaga aaaagtgaag
      541 ttctttgcaa ggtatgaccg catagcatcg gttaccatgg atggggcgga agatccctgg
      601 aacctttcaa gggacggatc atacatctat gccggaattg atttttcccc ggtaaagaat
      661 gtaaggatat ctcccaatta caacagctac atacccccgg cagagaattc tgacaatgtc
      721 ggaaccattg gcctcaacat agaggcaaaa ttctgacggt tccggcaatg gaaaggggct
      781 ctgcaaatca atgaatcata aaaaagatgt aaattcataa caaaacaagc atgaaaaaac
      841 agattttcat cctgatggcc gcaatggtac tgctgctcgt ctcatgcgga ggcggcggtt
      901 caaccaccgg caagcagaag cagatcaccc tttcagctgc cggggccacc ttcccgcagc
      961 cattctacaa cctcgccttc aaaaaataca atgaggcagg aggtccgctc accaactacg
     1021 gaggcatagg ttcaggcgga ggaatcaaga gccttcgcga ccagatagtt gatttcgcgg
     1081 ctactgacgc atacctttca gacgccgagc tggcagagat gcccggagag gtggttcata
     1141 tccccacctg catcggtgca gtggtgatag catacaatct tccgggcgtc acagacctgc
     1201 agctcacccc cgaactcctt gaagggatct tcctgggtaa cattaaaaag tggaatgatc
     1261 cgaagattct tgcagccaac ccgggtcttg ccgttcccga tatggagatc actgttgtct
     1321 accgttctga cggcagcggc accacataca tcttcagcga ctacatgtcg aaagtcagcc
     1381 cggcctggaa agctcagctt gggacaggca aagctctcaa ctggcctgca ggaataggcg
     1441 caaagggcaa tccgggcgtt tcaggaacca tcagccagag ccagggctcc atcggttaca
     1501 tcggttatga atatgctttc gcacagaaga tacccatggc taaagtgatg aacagcgcag
     1561 gtctattcat agagccgact ctggaatctt tcactgctgc tgccgctgca gagatgccgg
     1621 ctgacaccag ggtgatgctt accaactcgc ctaatcctgc tgcctatccc atcaccagct
     1681 tcacatggat catcatgtat aaggagctgg catatggcaa gcacagcagc gaggctgccg
     1741 aagcagcggt caaaaccgtg aaatggctga ccggtgaaga ggcacaggca attgccacca
     1801 gtgttcatta tgctgccctg ccgcagaacg cctctctgca ggccgcagag ctgctcaggg
     1861 ggataaccta ccagggtctg accgttctca gataaacaga tgtcaggaga cagggtcaca
     1921 aaggtactgc tgttcatctc atcactgaca atacttcttc tcagtgccgg gatagtcatc
     1981 tcccttttcg gaggggctct cccggcattc agggagttcg gattcaggtt catcacctct
     2041 tcagcctgga atccaaccgc aggaagcgag gagtacgggg ccatgtcatt catcgcgggg
     2101 acggtcatca catccatcct ggcactgata atatgcatac ccatggcctt ctccacctcc
     2161 ctcttcattg ccgaatacta caacggcacc cgcacctcag ggatactgcg gagcctggtt
     2221 gacctgctgg ccgggatacc ttcgatagtt tacggattct ggggctttta tgccctccgc
     2281 cccctgatgg tggctacagg cctgactgac cagggcttcg gggtattcac cgcctcactg
     2341 gtgctggcta ttatgattgt tccctatgcc acctcgctca gcgcggagat catctccatg
     2401 gttcccgccg agcttaagga agcagcctat tcactcggcg ccacacggcg cgaggtgatc
     2461 ttcagcgtgg tcatcccctc tgcccgctca ggcatcatcg ccagcttcat cctcgccctg
     2521 ggaagggccc tcggtgagac catggccgtg acgatgctga tcggcaatgc caacgtcata
     2581 cctaaaggac tctttgcacc gggaaacacc atggcttcgg taattgccaa ccagttcggt
     2641 gaggcatcag gactgaagat gagtgcactg atagccatcg gcctgcttct gtttctgata
     2701 acggccctgg tgaactatct cggcaaactg atcatcaaac gcttcgcatg acagagatgt
     2761 atcccgtact gactgacaat ataagcagga gaaaccggac caacatccgc ttccggtacc
     2821 tggtgatagc cggttcggtc ataaccgtca tccccatctt cctgatagtg ggcaaccttg
     2881 ctgtcaaggg attccggcag ataaacctcg cattctttac caagccggct cctgacacct
     2941 tccaggcaat gatggcagcc tcatcgggtg agatcatccc cggcggcata gccaacggcc
     3001 tcaccgggtc actgctgatg gttgtgctgg cgtcgctctt cgccataccg cttggactga
     3061 tgaccggaat ctatatctac gaaaacagca gtctgaagta tgcccggttc atcaggagcc
     3121 tcactgagat actgcagggt gtgccctcaa tcatactggg gatcattgcc tatatctgga
     3181 tagtaaagaa catcacaaga ggatattcgg cactggccgg gagcgtctcg ctggccatca
     3241 tgatgctgcc gcttatcata cgctccaccg aggagacgat gcgcatgata cccgtttcca
     3301 tcagggaggc agcatttgcg ctgggaactc catattacaa aatgatttat aagatactta
     3361 tacctgtcag cttcagcggc ctggcaacgg gagtgctcct ggcgctctca cgtatacttg
     3421 gtgagacggc tccgctgatg cttacagccc tcggcagccc tgccatcagc cttgatgtca
     3481 caaggccggt cagcgccgtg ccgctgctga tatgggattt ctacaatgat cccaacatgg
     3541 tcagcctggt ctggagcgcc tcactgttcc tgatggtatt cgtgctttca atgaacctcc
     3601 tcgcgcgaag cgtggcatca aaacataaat acatctgaac ctgatgaaca acacccctca
     3661 aagtaataat gtactggaga tcagggatct gtctattgcc tatgaacccg ggaaaaatgc
     3721 cgtgaatggt gttaccgctt cagtgaagcg aaacagcatt accgccatca tgggtccctc
     3781 gggttgcggt aagagcaccc tgctgcgggc catgaaccgc atgcacgagc tctatcccaa
     3841 catccggaca aaaggtgaca ttctgcttga cggtcagagt atcttctcaa tgtcagtaat
     3901 agagcttcgc cgtaaggtgg gaatggtctt ccagcgtccc aacccattcc caaacatgac
     3961 tatctatgaa aacgtggtgg ccggtttcag cctcaacgac atacgtctga aaaaaagcca
     4021 gcgcgacgag atagttgaaa acgcccttcg caacgtgacc ctctgggacg aagtgaagga
     4081 gtcgctctac agaaaaggga ccttcctctc cggagggcag cagcagcggc tctgcgtggc
     4141 cagggccatc gctttcaacc ccgaggtgct gctcctcgac gagcccacct ccgcccttga
     4201 ccccatcgcc acatcgaaga ttgaagacct gctgggagag ctgaagaaga aatacaccct
     4261 ggtgcttgtg acccataaca tgtcacaggc agcccgtatt tcagacgact cgatgttcat
     4321 gtaccttggg gagctggtgg agtcgggccg caccggtaca ctctttacca ccccgaaaga
     4381 cccgcgtact gaagcatatc tgaccgggaa attcggatga ttagggacgt tttttataca
     4441 tttgctaaaa cacttgatta tgagtgagaa aaaagaacag gcactgaatg agataaagac
     4501 cagctttggc gagatggcaa gtcttgtact ccggcagctg accctcctgg agaagctgat
     4561 gggcacaact gaagaacagg cattcactga tctgattgat gagatggaaa aggccgagag
     4621 ccgcattgac aacctcgagg tggttattgg cgagcaattt accagcacca ttgtgcttta
     4681 ccagccggtg gcctccgacg tgcggcgcct ggtggcaata taccgcatga ccattaacct
     4741 tgagcgcatc ggggacagga tacataacct ctgccggatc atcggcgata tcaggaacac
     4801 aaccgagttt acctcaaccg cgtctctgat cacaaacatg ctcacctctg gaaccatcat
     4861 ggtggaaaag gcactgctct cattcaccaa ttcagatcct gactatgcta tctggacaat
     4921 caagaatgac acggtggttg atgagatgaa tcacaagctg ctgcttggcc accttcgcaa
     4981 ggagaatatt gatgaccgga cccggtcaat ggtacagagc tacattgagc tgaaagatat
     5041 gatatccaat attgagagaa tagctgatca tgccaccaac attgcagagg catcgatcta
     5101 ctccctgcag ggaacagata tcaggcatac cgggatcggg aaagaagagt agtgaagtct
     5161 gaaggtctga aggtctgaag gtctgaaggt ctgaagtctg aaggtctgaa ggtctgaag
//
LOCUS       DAOPXU010000152         5161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_20345_length_5161_cov_11.258911, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000152 DAOPXU010000000
VERSION     DAOPXU010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20345_length_5161_cov_11.258911"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2885)
                     /locus_tag="PKK03_11440"
     CDS             complement(<1..2885)
                     /locus_tag="PKK03_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNX85053.1"
                     /translation="MKKSCLSLVLAVCALMIYPSPVTGAENNVPWKNFTGDPRVDSIL
                     NTMTVREMIAQLIWVPAWADEKGGNYRQVELLVEKYGIGGVIFFEGSRDMQLDYTKRI
                     SSISRIPLIIAQDAEWGTGMRLREVEDFPYQMTLGALQDDSLIYRMGAAVAWQCHDIG
                     VNLNLAPVADVNNNPMNPVINYRSFGEDPEKVAEKATMYMKGLQDNGIIACAKHFPGH
                     GDTDTDSHTGLPVIRGDRSRFDRIELIPFRRLADEGIGAVMTAHISVPGLDAGGLPAT
                     FSKKVVTGLLRQESRFRGLILTDAMNMAGATMTFPSGIADAEALLAGNDIIEYSTDPI
                     RAIEEIARRVEKGEIAVSEITDRCRKLLEAKLWLEIHRAENGGKAESGDQAIPGPASE
                     AAAGFASAPGGIPVSGHPALIRDLYAGAMTLIENNDNLLPLGRLDRIRIATVSVNRLA
                     MTEFQRMTDRYTNADHYFIDPANEQGARFVMSKLKDYDVVIAGFCALEQKPAGLYGVT
                     PALNSLFRQIAALDRAAVIWFGNPYGVARLDMTKKPTAMLVAYQDNSYTHQVAVQVLF
                     GAIGASGRLPVTVNENYPAGTGIKTPGNIRMQFGFPENAGLSSVRLISKVDSIVSAGI
                     DTLAFPGCQVLIARRGIVVLDKCYGFHLYDSTEAVTENDLWDLASVTKVSAATPSLML
                     LDDRGLFDIDKTLGDYLPWFRFSDKGDMVLREMLAHQAGLKAWIPFWRNTLENDSTFR
                     RGIFSDYTNRKYALQVTDSIYMNRHYLKDMFREIRDSPVGDKKYVYSDLTFILAAEII
                     ESLTDSTIDKFAPANIYRPIGAFNITYRPLEKYPAERVVPTEYDSLFRKQLLRGTVHD
                     EGTAMLGGVSGHAGLFATGNDLLKLVEMYRRGGEYGGVRVLSSDVLRKYTTVQFPDND
                     NRRALGFDKPLLRNDTVPPEEAYPCPSASPASFGHSGFTGTFIWCDP"
     gene            2993..4120
                     /locus_tag="PKK03_11445"
     CDS             2993..4120
                     /locus_tag="PKK03_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HNX85054.1"
                     /translation="MAATKSSGKSAGKSARLLSLDVFRGMTVAFMIIVNTPGTWSHVY
                     APLRHASWHGCTPTDLVFPSFLFISGVAMFYSLRKYNFEFSGPSLFRILRRVLLIFAA
                     GLFLNIFPHFVRDYSTLRIMGVLQRIALAWGLGAILVLLIRRNYIWLATAVILFGYWA
                     LMHFMGGADPYSLEGNYARTVDIAVLGENHLYRGFGMPFDPEGLLSTLPATATVLLGF
                     MAGGLISANGSSWKTPGWLALTGTLLIGAGLLWGQYFPINKPIWTSSYVIYAGGIGMV
                     ILALLFIIIDIWNLKGWTGFFNTFGTNPLFTYLLAGIWTKTMLAVKIGETTLYKWIFE
                     HICSPLFEEQKLASLLFAIMQVLIIWAFGYILYRKKIIIRF"
     gene            4122..>5161
                     /locus_tag="PKK03_11450"
     CDS             4122..>5161
                     /locus_tag="PKK03_11450"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNX85055.1"
                     /translation="MDIRTAVEKAFERWKGSSPEIITRLPQSGSDRVYFRLICREDQV
                     IGAYNPSREENEAFVGFSNHFRSKGLPVPEIYVYYPEDKIYFLEDLGDTNLFTWLARR
                     SDEERFNRETENLFLTVAGDLVRIQTEGIRGLDLSLCYPHRSFDRQSIVWDMNYYKYM
                     FLKLIGAPFNETRLERDFEILTRYLLDAGQEYFLFRDFQTANIMIRDGKPWYIDYQGG
                     RLGAPQYDIASLVYDAKAYIPEKIRKTTINRHLDLFAAATGRPRESLEMYQGAFTLIR
                     LMQALGAFGFRGLHENKPTFNESIVPAVELMNELLESGDVRIDLPELKKASLAVPLQR
                     KYRILEHYQDMVT"
BASE COUNT         1197 a         1428 c         1279 g         1257 t
ORIGIN      
        1 tccggatcac accagatgaa ggtgccggtg aaccccgagt gcccgaaact ggcaggcgag
       61 gccgacggac agggataagc ttcctccgga ggcaccgtat cattccgcag cagcggtttg
      121 tcaaatccca gtgccctgcg gttatcgttg tccgggaact gaacggttgt gtacttcctg
      181 agcacatcac tgcttagaac tctgacacct ccgtattcac ctcccctgcg gtacatctcc
      241 actagcttca ggaggtcatt gcccgtggca aacagaccgg catgacctga cacgcctccc
      301 agcattgctg tcccctcatc atgcaccgta cccctgagca actgcttcct gaaaagagaa
      361 tcatactcag tagggacaac cctctctgcc ggatacttct caagcggcct gtaggtgatg
      421 ttgaaggcac cgatgggacg gtagatattt gccggtgcaa acttgtctat ggtgctgtcg
      481 gtgagactct ctatgatctc tgcagcaagg atgaaggtga gatcggaata gacatatttt
      541 ttatcaccca caggactgtc acgtatctcc ctgaacatat ccttcaggta atgacggttc
      601 atgtagattg agtcagtaac ctgtaaggca tacttccggt tggtataatc gctgaaaatg
      661 ccccgcctga aagtgctgtc attctcaagg gtatttctcc agaagggtat ccatgccttc
      721 agaccggcct ggtgggcgag catctcgcga aggaccatgt cacccttgtc ggaaaaacga
      781 aaccagggaa ggtaatcacc aagggttttg tcaatgtcaa acaaacctct gtcatcaagc
      841 agcatcagag agggagtggc cgctgatacc ttggtcactg aggcaaggtc ccacaggtca
      901 ttttctgtga cagcctcggt gctgtcgtag agatggaatc cgtagcattt gtcaagcacg
      961 acgattcccc ggcgtgctat tagcacctgg caacccggga aagccagggt gtcaatgcct
     1021 gcactcacga tggaatcaac tttgcttatc agcctcaccg aggaaagtcc tgcattctcc
     1081 ggaaacccga actgcatcct gatgttgccg ggggttttga tgcccgttcc tgccggataa
     1141 ttttcattta ctgtcaccgg cagcctgcct gatgcgccga tggcgccgaa gagcacctgc
     1201 accgcaacct ggtgagtgta actgttatcc tggtatgcca caagcatagc cgtcggcttc
     1261 ttcgtcatgt caagccgcgc aacaccatag ggattgccga accatattac agcagccctg
     1321 tcaagtgcgg ctatctgccg gaaaagactg ttcagggccg gcgtgacgcc ataaagccct
     1381 gccggcttct gctccagtgc acagaaacct gctatgacca catcataatc ctttagtttt
     1441 gacattacaa acctggcgcc ctgctcattt gccgggtcaa tgaagtagtg gtcagcattg
     1501 gtatacctgt cggtcatacg ctggaattcg gtcatagcca gtcggttgac agagacagtg
     1561 gctattctta tcctgtcaag cctgcccagc ggaaggagat tgtcattgtt ctctatcagg
     1621 gtcatcgctc cggcatacag gtcacgtatc agcgccgggt gcccggaaac ggggatgccg
     1681 cctggggctg aggcgaagcc ggcagctgcc tctgatgccg ggcccggaat tgcctgatcc
     1741 ccggattctg ctttacctcc attttccgcc ctatgaatct ccagccacag ctttgcttca
     1801 agaagcttcc tgcacctgtc agtgatctca cttactgcta tctcgccctt ttcgaccctg
     1861 cgggcaatct cctcgatggc acggatagga tcagtggagt attctattat gtcattcccc
     1921 gccagcagcg cctcggcatc ggcaatgccc gacgggaagg tcatggtcgc accggccata
     1981 ttcatcgcat ctgtaaggat gagccccctg aaacgcgact cctgcctgag cagtccggtc
     2041 accactttct tcgagaaagt cgccggaaga cctccggcat ccagtcccgg cacacttata
     2101 tgcgctgtca taactgcccc gattccttca tctgccagcc tcctgaaggg tatcagctca
     2161 atcctgtcaa aacggctgcg gtcacccctg attacaggaa gtccggtatg ggagtcagtg
     2221 tcagtgtcac cgtgacccgg gaaatgcttt gcacaggcta tgatgccgtt atcctgcagc
     2281 cccttcatat acattgtcgc cttttctgca accttctccg gatcctcacc gaaggagcgg
     2341 tagtttatta ccggattcat agggttgttg ttcacatcgg caacgggtgc aaggttcagg
     2401 tttaccccaa tgtcatggca ctgccacgcc acggcagcac ccatcctgta aatcagggag
     2461 tcatcctgca gcgctcccag cgtcatctgg tacggaaagt cctccacctc cctcagccgc
     2521 atcccggttc cccactcagc atcctgtgca atgatcagcg gtatccgcga gatactgctg
     2581 atccttttcg tgtaatccaa ctgcatatca cgtgatccct cgaagaaaat cacacccccg
     2641 ataccatact tttcaaccag cagctctacc tgcctgtaat tacctccctt ttcgtcagcc
     2701 catgccggta cccatattag ctgagcaatc atctccctga cagtcatagt gttaagtatg
     2761 ctgtcaaccc tggggtcgcc ggtgaaattt ttccacggaa cattgttctc cgctccggtg
     2821 accggagagg ggtagatcat cagggcacac acggcaagta ctagtgataa gcaggatttt
     2881 ttcatcagag gaatgaatta tgcgttgtac caaaattagc atttttcttt atgattcttt
     2941 tcccggttga ttggttccaa tttgttattt ttacccaacc aacagaacgc atatggcagc
     3001 aacaaaaagt tccggaaaga gcgccggcaa atcagcgcgg cttttgtcac ttgacgtttt
     3061 cagggggatg actgtggctt tcatgatcat tgtcaacacc ccgggtacat ggagtcacgt
     3121 atatgctccc ctgagacatg cctcgtggca tggctgcaca cccactgacc ttgtatttcc
     3181 ctcattcctg ttcatctcgg gtgtggcaat gttctattcc cttcgaaagt ataacttcga
     3241 attctccgga ccatcactgt tccgcatact gcgaagggta ctgctcatct tcgccgcagg
     3301 cctttttctg aatatttttc cccattttgt acgtgattac agcacactcc ggatcatggg
     3361 agtgctacag aggatagcac tggcatgggg ccttggtgca attctggtgc tgctgatccg
     3421 caggaattat atctggcttg ccacggcagt aatactgttc ggatactggg ccctgatgca
     3481 tttcatggga ggggccgatc cctattcact cgagggtaac tatgcacgga cagtcgatat
     3541 cgccgtcctc ggcgaaaatc acctttacag gggatttggt atgccgtttg acccggaagg
     3601 actgctgagc acccttcccg ccaccgctac ggtattgctc gggtttatgg ccggaggatt
     3661 gatcagcgct aacggatcat catggaaaac cccgggatgg cttgccctca caggcaccct
     3721 gcttatcggg gcaggactgc tatggggaca gtatttcccc atcaacaaac ccatctggac
     3781 aagctcctat gttatctacg ccggcggtat tggtatggtg attctggccc tgctgttcat
     3841 aataattgac atatggaacc tgaaaggatg gacaggcttc ttcaatacat tcggcacaaa
     3901 tcccctcttc acctacctcc tcgccggaat ctggaccaag acaatgcttg ccgtgaagat
     3961 aggggagacc actctttaca aatggatatt cgaacatatc tgcagtccgc tctttgagga
     4021 acagaaactt gcaagcctgc tcttcgcaat aatgcaggtt ctgatcatat gggctttcgg
     4081 ctacatactt tacaggaaaa agataattat caggttctga aatggatata agaacagcag
     4141 tagaaaaggc atttgagcgc tggaaaggct cctctccgga gatcatcacc cgcctgccgc
     4201 aatcgggctc agacagggtt tatttcaggc tgatatgccg tgaggaccag gtgatcggcg
     4261 catacaaccc gagccgcgag gagaacgaag ccttcgtcgg attttctaac cacttcagat
     4321 caaagggatt accggtgccg gagatttatg tatattatcc cgaggataag atctattttc
     4381 ttgaagacct gggtgatacc aaccttttta cctggctcgc ccgccgttcc gatgaggaac
     4441 gtttcaacag ggagacggaa aacctcttcc ttacagtcgc aggtgacctg gtacgcatac
     4501 agactgaagg gatcagaggg cttgacctgt cgctgtgtta tccacaccgc agcttcgacc
     4561 ggcagagcat agtctgggat atgaactatt acaagtacat gttcctcaag cttataggag
     4621 caccgttcaa tgagaccagg cttgagcggg attttgagat tctgacccgt tacctgctgg
     4681 atgcaggaca ggagtatttt ctcttccgcg acttccagac ggccaatatc atgatcaggg
     4741 acggaaagcc gtggtatatt gactaccagg ggggcaggct cggggcgccg cagtatgaca
     4801 tcgcctccct cgtttatgat gccaaggcct atatcccaga aaagatcagg aagacaacaa
     4861 tcaacaggca cctggatctc ttcgcggcag ctaccggcag accacgcgag tcacttgaga
     4921 tgtaccaggg cgccttcacc cttataaggc tcatgcaggc cctgggcgcc ttcggattca
     4981 ggggactgca tgagaacaaa cccactttca acgagagcat cgtaccggct gttgagttga
     5041 tgaacgagct tcttgagagc ggtgacgtca ggattgacct ccccgaactg aagaaagcct
     5101 cactcgcagt gccgctgcaa cgaaagtacc gcatcctgga acactaccag gatatggtta
     5161 c
//
LOCUS       DAOPXU010000153         5018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_21154_length_5018_cov_11.892404, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000153 DAOPXU010000000
VERSION     DAOPXU010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21154_length_5018_cov_11.892404"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /gene="hydG"
                     /locus_tag="PKK03_11455"
     CDS             <1..1425
                     /gene="hydG"
                     /locus_tag="PKK03_11455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HNX85056.1"
                     /translation="EMKFNPEKCSIPDRRMEPFIDPAELWAHINNNKADAVRVRELIA
                     KSLSKERLTLAETAALINAEGDLVEEIKAGARELKKKVYGNRIVLFAPLYIGNKCINN
                     CQYCGFRTSNKDAKRKTLTDTEIIAEVEALEDNGQKRLILVYGEHPEYGPEYIAHTVR
                     LVYGIKKDRGEIRRVNINAAPLDIDGFRTVGRAGIGTYQIFQETYDPEAYKWYHLGGK
                     KRDYQWRLTALDRAQEAGIDDVGIGALFGLYDWRFEVMGLVRHTNHLEACYNVGPHTI
                     SFPRIKDASSLNLNGKYFTSDDDFKKLIAILRLAVPYTGLILTAREAPDVRSEAMAFG
                     VSQIDGGTKLELGSYSASRNEEQNLNREQFKINDARSLADVINELIDNDYIPSFCTAC
                     YRLGRTGEHFMEFSVPGFIKRYCTPNAILTLSEYIVDYASPELAEKGWKAIEKNMVDL
                     DEAMKQSIRKKIERIRNGERDLYY"
     gene            1437..1961
                     /locus_tag="PKK03_11460"
     CDS             1437..1961
                     /locus_tag="PKK03_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85057.1"
                     /translation="MVRVLGIKITDRIKEAGLVQKALSEHAAVVSTRLGFHELNDELC
                     SREGFMVIHLAGNSDDCEDLAAKLKGIEGLFVREIIFSHGLSDAPVECEGTTILGIMV
                     PRRDDLGPAVQALLTTYGCVIRTRLGVNEVYFGEPAGLIILELAGDRSQYLSLERSLR
                     EIDDIAIGRICFPA"
     gene            complement(2113..3204)
                     /gene="hydE"
                     /locus_tag="PKK03_11465"
     CDS             complement(2113..3204)
                     /gene="hydE"
                     /locus_tag="PKK03_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HNX85058.1"
                     /translation="MSSGIASLLDRKVHDRDDILALLQCSEDEAPLLYRRAAAVRDRY
                     VGNHVYLRGLIEYSNICGKNCLYCGVRRDNVRIERYTLTREEVLEAAMTAYNLKLGSI
                     AIQSGENSSKAFIDTIEELVSTICRMTDNRLGITLSLGEQSRETYKRWYDAGAHRYLL
                     RIEASSQALYRRVHPDDDMHRYQRRLDCLKAIQDTGYQTGTGVMVGLPYQTMSDLADD
                     VIFMRDFDIDMCGMGPFIEHSDTPLGERGTDNLFLKERFNLTLRMIAIVRIIMKDINI
                     VASTAMQTIDPAGREKAISCGANVVMPNLTPARYRAGYQIYEGKPGYREVNESNVSGL
                     NLDLIPGITLGLGTWGDTPHYGRRLTGKR"
     gene            complement(3272..4900)
                     /locus_tag="PKK03_11470"
     CDS             complement(3272..4900)
                     /locus_tag="PKK03_11470"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNX85059.1"
                     /translation="MKNQTLSAVIRTGLILLLAVLPLLAAGQFAKDIDPDGPGYAENC
                     TSIMVGRLASTDGSVMTSHTCDGRYRTWLEVVPAMTFARDTVHAVYWGTMQTEEAWDM
                     TNVTRKGEIPEVKSTYAYLNTAYPCLNEKQLAIGETTIYGKRELINQDGLFLIEELER
                     IALQRCTTARQAIALIGSLAEQYGYGDMAECITIADPKEVWQLEIAGSGPGKPSALWV
                     AQRIPDDHIGVAANIPRISDVNFKDTDKFMTSTDLRKKAKDLGYWDGKDPFKFYRVIG
                     TGKPYAIREFFVLNTLAPGLNLTMEMEELPFSVKPEKKLSVSDVMALFRETYEGTPYD
                     MTRNLMVTVRRRDKDGKEVTETVKSPAINNWMNNDIRTLINELKPGTLDRQRTIAIAG
                     CSYSHVIQCREWLPDEVGAVAWFSFDNPGQSPRIPVFSGTMSLPQSFRICGQHRYRED
                     AAIWAFREANRLSAVNWERGRRLIEPAVAEQEHKALAELPELEERVKSMVKEGRHKEA
                     REAVTGYSNAFAYGAMNRWEELKRELWQMFGRGF"
BASE COUNT         1133 a         1309 c         1343 g         1233 t
ORIGIN      
        1 gaaatgaaat tcaatcctga aaaatgcagt atacctgacc gcaggatgga gccgttcatt
       61 gatccggccg aactgtgggc acatattaat aataacaagg ctgacgccgt aagggtgagg
      121 gaactgatag caaagtcgct ttccaaggag cgcctcactc tggctgagac agctgcactc
      181 atcaacgccg aaggcgacct ggttgaggaa ataaaggcag gcgcacgcga gctgaagaaa
      241 aaagtctatg gcaacaggat agtgttgttc gcaccccttt acataggaaa caagtgcata
      301 aacaactgcc agtactgcgg tttcagaacc tccaataagg acgcaaaacg caagaccctt
      361 accgatacgg agatcatagc ggaggttgaa gccctggagg ataacggcca gaagagactg
      421 attctcgttt acggtgagca cccggagtat ggcccggagt acattgcaca tacggtgcgc
      481 ctggtttatg ggatcaagaa agaccgcggt gagatccgca gggtgaacat caacgctgcc
      541 ccgcttgaca tagacgggtt ccgcaccgtg ggccgggccg ggataggcac ataccagata
      601 ttccaggaga cctatgaccc tgaggcatac aaatggtatc acctcggcgg aaagaaaagg
      661 gattatcaat ggcgcctgac agctctcgac agggcccagg aggcaggtat tgacgacgtg
      721 ggcattggcg ccctcttcgg cctctatgac tggcgcttcg aggtgatggg cctggtaagg
      781 catacaaacc atctggaagc ctgctacaat gtaggcccgc ataccatctc attccctcgc
      841 ataaaggatg cctcgtcgct gaacctcaac gggaagtact tcaccagtga tgacgacttc
      901 aagaagctta ttgccattct tcggcttgca gtgccttata cgggactgat actcactgcc
      961 agggaggctc ctgatgtccg ttccgaggct atggcattcg gggtgtcaca gatagacgga
     1021 ggaaccaaac ttgaactggg cagctactca gcctcaagga atgaggaaca gaaccttaac
     1081 cgcgagcagt ttaagataaa tgacgcgagg tcactggccg atgtcatcaa tgagctgata
     1141 gacaatgact atatcccgtc attctgcacg gcatgctacc ggctgggccg caccggcgag
     1201 cacttcatgg agttctcggt gccgggcttc atcaagaggt actgcacccc caacgccatc
     1261 cttactctct ctgagtacat cgttgactac gcttcacccg aactggcaga gaagggctgg
     1321 aaagccattg aaaagaacat ggtcgacctc gatgaggcaa tgaagcagag catcaggaaa
     1381 aaaatagagc ggatcaggaa tggcgagagg gatctttact attaaaatca acaatcatgg
     1441 tcagagtatt aggaattaag attactgaca ggatcaagga ggcagggctg gttcagaagg
     1501 cactttctga gcacgctgcc gttgtcagca caaggctcgg gttccacgaa ctgaacgatg
     1561 aactctgcag tcgcgaaggt tttatggtaa tacatcttgc aggcaattcc gatgactgtg
     1621 aggatctggc tgcaaagctg aagggaattg aggggctgtt tgtcagggag ataatattct
     1681 ctcacggcct gtcagatgct cctgtggagt gtgagggcac caccattctc gggatcatgg
     1741 tccccaggcg tgatgacctt ggtcctgctg tacaggcgct gctgactacc tacgggtgtg
     1801 ttatccgcac caggctgggc gtcaatgagg tttatttcgg agaaccggcc ggacttatca
     1861 tccttgaact tgcaggcgat cggagccagt atctttccct tgagcgatcg ctgcgcgaaa
     1921 ttgatgatat agccatcggc cgtatctgtt ttcctgcctg accaagacaa ctcctgtccg
     1981 gaccggcttc cctatctgag tgtctgtctg aagaagacgg cgttgataaa aagtttcgag
     2041 gtgccatacc tcaccggcat cgccggtggg cgtgtccttt ctggcagaat cttttcagtg
     2101 acacaggtat tatcatcttt ttccggtcag cctcctgccg tagtggggtg tgtcacccca
     2161 tgtccccagc ccgagggtga ttccgggtat cagatcgagg ttaaggccgc tgacatttga
     2221 ctcattgacc tcgcggtagc ccggcttgcc ctcatagatc tggtatcctg ccctgtacct
     2281 ggcaggggtg aggttcggca ttaccacgtt ggcaccgcag gaaatagcct tctcccggcc
     2341 tgccggatca attgtctgca tggctgttga agcgacgata ttgatatcct tcatgatgat
     2401 cctgacaatt gctatcatcc tgagggtgag gttgaaacgt tccttaagaa agagattgtc
     2461 ggttcccctt tcccccagcg gggtgtcaga atgctctatg aatggtccca tcccgcacat
     2521 gtcgatgtca aaatcgcgca tgaagatgac atcatcagcc aggtcgctca tagtctggta
     2581 cggcagccct accattacgc cggtacctgt ctggtatccg gtatcctgaa tcgccttcag
     2641 gcaatcaagt ctcctctggt acctgtgcat gtcatcgtca ggatgcaccc tccggtagag
     2701 tgcctggctt gatgcctcta tcctcagaag gtaacggtgc gcccccgcgt cataccatct
     2761 cttgtaggtt tccctgctct gctcgccaag cgagagtgtt atgccgagcc tgttatcagt
     2821 catcctgcat attgtggaaa caagctcttc aatggtgtca ataaatgctt tggaggagtt
     2881 ttctcctgat tggatggcaa ttgacccgag cttgagattg tatgcggtca tggctgcttc
     2941 aagtacttct tcccgtgtca gggtgtacct ttctatcctg acattgtcac gcctgacacc
     3001 gcaatagaga cagttctttc cgcagatgtt cgaatactcg atgagtccgc gcagataaac
     3061 atgattgccc acataccggt ctctgacggc tgctgcacgc ctgtagagca ggggagcctc
     3121 atcctcgctg cactgaagaa gtgcaaggat gtcatccctg tcatggactt tcctgtcaag
     3181 aagtgaggct atgcctgaac tcattgctat tgtgaatcgt ttaacgttaa caacacggaa
     3241 gatgataagt tcagggcagg gggcattatg atcaaaaacc tctgccgaac atctgccaga
     3301 gttctctttt caattcctcc cacctgttca tggcaccata tgcgaaggcg ttcgagtatc
     3361 ctgtcacggc ttccctggct tctttatgcc tgccctcttt aaccatgctc tttactcttt
     3421 cctccagttc gggcagttct gccagtgcct tatgctcctg ctcagctact gccggctcga
     3481 tgagcctgcg gccacgctcc cagttaaccg ccgagagcct gttggcttcg cggaaggccc
     3541 atatggcggc atcttcacgg tagcggtgct gcccgcagat gcggaagctc tgcggcaggg
     3601 acatcgtccc tgagaagacg ggtatgcgcg ggctctggcc gggattgtca aatgagaacc
     3661 atgccactgc gccaacttca tccggcagcc actcacggca ctgaatcacg tgcgagtagg
     3721 agcagcctgc gatggcaatg gtgcgctgcc tgtcaagggt gccgggcttg agctcattga
     3781 tcagggtgcg tatgtcattg ttcatccagt tattgatggc aggcgactta accgtttcgg
     3841 taacctcctt gccatcttta tcccttctcc ttacagtaac catcaggttc ctcgtcatgt
     3901 catacggcgt gccttcgtag gtttcgcgga agagcgccat aacgtcactg acagagagct
     3961 ttttctcagg tttcaccgag aacgggagtt cttccatctc catcgtcagg ttaagtccgg
     4021 gggcaagggt gttcagtacg aagaattcgc ggatggcgta gggtttacct gtgccgatca
     4081 ccctgtagaa cttgaacggg tcctttccgt cccagtaacc caggtccttt gcctttttac
     4141 ggaggtctgt tgatgtcatg aatttatcag tatccttgaa gttgacatct gagattctcg
     4201 ggatgttggc ggcaacgcct atgtggtcat caggaatcct ctgtgccacc cacagggctg
     4261 atggcttgcc cggacctgag ccggctatct caagctgcca cacctccttc gggtctgcta
     4321 tagtgatgca ttcggccatg tctccgtacc cgtactgttc cgccagcgag ccgatgaggg
     4381 ctattgcctg gcgtgccgtt gtgcagcgct gaagcgcgat cctctcaagc tcctctatca
     4441 ggaagaggcc gtcctggttt atcagttccc tcttaccgta aatggttgtt tctccgatgg
     4501 cgagctgctt ctcattgagg catggatatg ccgtgttgag gtatgcatat gtcgatttca
     4561 cttccgggat ttcccccttg cgtgtcacgt tggtcatgtc ccatgcctcc tcggtctgca
     4621 ttgtgcccca gtaaacagca tgaacggtgt cccttgcgaa agtcattgcc ggaacaacct
     4681 ccagccaggt gcggtacctg ccgtcacagg tgtgggaggt cataaccgag ccatcggttg
     4741 aagccagccg gccgaccatg atgcttgtgc agttttcggc gtagccggga ccgtcagggt
     4801 caatgtcttt tgcgaattgt ccggcggcaa gaagcgggag tactgccaga aggaggatta
     4861 atcctgttct gataacggca gataaggtct ggtttttcat aattagtcgt ttgctgacaa
     4921 aaatagtcag gttattcaac ttaatgctgc caaagataat attctgcagc ataatgccgc
     4981 ggtgttgttg ccaggtatga aatgcacaga tcatgata
//
LOCUS       DAOPXU010000154         4958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_21553_length_4958_cov_14.106669, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000154 DAOPXU010000000
VERSION     DAOPXU010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21553_length_4958_cov_14.106669"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PKK03_11475"
     CDS             complement(<1..898)
                     /locus_tag="PKK03_11475"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNX85060.1"
                     /translation="MSIWDEIKGSFRHGTALTRLMYINGAIFLLIKIIEVIGVLTSSP
                     GLATAVISNLAVPASTSALALKPWTPVTYMFTHQGFIHLLFNLLWLWWFGKIFLSYLD
                     QRKLVSLYIMGGLAGALLYIIVFNIFPAFASAVNVSIALGASASVMALVIATAAYLPD
                     LELHLLFFGRVKLKYLALVTFLITSVFDFSVNTGGKIAHIGGAVMGFAYGQGLRNGKD
                     FGAWLNRILDFFVNLVKPGKRLRVKYKKTGASFNAQKTKTDHEYNSVKAERQKEIDHI
                     LDKISKGGYDSLTREEKAILFSE"
     gene            complement(898..1632)
                     /locus_tag="PKK03_11480"
     CDS             complement(898..1632)
                     /locus_tag="PKK03_11480"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNX85061.1"
                     /translation="MNRILSGTPPVVKNLIIINVVMLLLNELLRLTSGINLNGILGLY
                     FPKSEFFRSWQIVTHMFMHGGFVHLLFNMFALWMFGRILEQVWGPKRFLLYYLVTGLG
                     AAFFFELVQWIQYNKLMGILSPEQLQYVYDMGGNAVSQGKNFTDNQMGMLNSILNVPV
                     VGASGAVYGVLLAFGMLFPNTQLMFIFPPIPVKAKYVVIGYAAIELYLAITSPGSGIA
                     HTAHLGGMIFGYLLIRYWRKTTKTLY"
     gene            complement(1685..3529)
                     /gene="mutL"
                     /locus_tag="PKK03_11485"
     CDS             complement(1685..3529)
                     /gene="mutL"
                     /locus_tag="PKK03_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNX85062.1"
                     /translation="MSDIIKLLPDSVANQIAAGEVIQRPASVVKELTENAVDAGATEI
                     SVVIRDAGRTLVQVIDNGCGMSETDARLSFERHATSKISSADDLFIITTKGFRGEALA
                     SIAAVSMTELRTRREEDEAGTLVEINNSRVTRQEPCSCAAGSVFSVRNLFYNVPARRK
                     FLKSDTTEFRHIITEFQRVALAHPEIRFNLTHNDQEIYRLPAGNHRQRIAGLFGKHYN
                     QNLVSVETSTSIVTVSGFAGKPENARRSAGEQFFFVNNRFIRHPYLHRAVLEAYQGLL
                     PPDTLPAYFIYMTADPATIDVNIHPTKTEVKFEDERSVWQIMLASVREALGRFNVVPS
                     IDFGPEGAVEIPVIMGKTPPPPLPQIEVDHTFNPFEGTEYQRPETKWKWDAERNLPRD
                     WESLFTITARSGNKSESESGDDMSTGRSADSGQATGQRRFFQVKNRYIMCPVISGIMM
                     IDQRRAHERVLYEKYLTSLGDGPKPAQVSLFPVEAELNPGDMVIIEEIGDQLRMLGFE
                     IKAGKNGRVTITGHPAASRNANPLSMLETIIAEYKHSMGDPAIGARERIALSMARASA
                     IQYGTPLTHTEMEELFDMLFACALPNYSPSGKTVMNIITLEELDRRFS"
     gene            complement(3539..3874)
                     /locus_tag="PKK03_11490"
     CDS             complement(3539..3874)
                     /locus_tag="PKK03_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85063.1"
                     /translation="MAIPSFFKQNKPRGFNYRPRYYDPVKEELEQRRRAWAEEERKGG
                     EEQKNSESSFTEATEEGARQPYRSRIMRGDMKNYFNRRQEKVQKQTTIRLIVIIIILA
                     FVVYLYLRF"
     gene            3995..4522
                     /locus_tag="PKK03_11495"
     CDS             3995..4522
                     /locus_tag="PKK03_11495"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNX85064.1"
                     /translation="MKILVTHAGTSGLSASVAERVARVLTGLGAEVTVRPMSGVSTIK
                     GFSAVIAGCQEQGGGWVPEAMEFVRRNQSALGKKPFAIFTVIPSLGTDKGAESRMVIM
                     QYTAHVRGMVHTVSEGFFSGETENRGAPQPGERSKFKLRLLLGLLREVETRSNSEIET
                     WSAQVHEKLVKLLAK"
     gene            complement(4614..>4958)
                     /locus_tag="PKK03_11500"
     CDS             complement(4614..>4958)
                     /locus_tag="PKK03_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85065.1"
                     /translation="IYAKQVVNDSVIATFPDSYQGGQVKMWCNNYWMFVGQYKQDTII
                     SESYGGGSYTLEGIVYKETIKYHVTDDYIGETLRMRIVVANDTLIQVWPADETGEVDK
                     SNYWSEKYIRVK"
BASE COUNT         1062 a         1243 c         1378 g         1275 t
ORIGIN      
        1 tctcgctgaa gaggattgct ttttcttccc tcgtgagact gtcataacct cctttcgaaa
       61 tcttgtcgag tatatggtct atctccttct gcctttcggc ttttacagag ttgtattcgt
      121 ggtcggtttt cgtcttttgt gcattgaagg aggctccggt tttcttgtat ttaaccctga
      181 gccttttccc cggcttgacc agattaacaa aaaagtcgag gatcctgttt aaccatgccc
      241 caaaatcctt gccatttctc aaaccctgtc cgtacgcaaa tcccatcacc gcaccgccga
      301 tgtgggcaat cttgccgccg gtattaaccg aaaaatcaaa tactgaggtg ataaggaatg
      361 ttaccagcgc caggtatttg agcttcaccc ttccgaaaaa caggaggtgc agctcgaggt
      421 cgggaagata tgcggccgtg gctatgacca gcgccattac cgaggctgag gcgcccagcg
      481 caattgagac atttaccgct gacgcgaagg ccgggaagat attgaagaca atgatgtaaa
      541 gaagcgctcc ggcgagacct cccatgatat aaagtgacac cagcttccgc tgatcgagat
      601 atgacaggaa tattttcccg aaccaccaca accacagaag gttaaagagc aggtggatga
      661 atccctggtg ggtgaacatg taggtaaccg gggtccatgg cttgagggcc agggcggagg
      721 tggaggccgg gaccgccagg ttcgatatta ctgccgttgc caggccggga ctggaagtga
      781 gtacccctat tacttcaatg atctttataa gcaggaaaat ggcgccgttg atgtacatca
      841 gccgcgtcag ggcggtgcca tgtctgaatg agccctttat ctcgtcccag atgctcatca
      901 gtaaagggtt ttagtggtct tgcgccaata cctgatgaga aggtagccga agatcatccc
      961 gccgaggtgg gcggtatggg caatgccgct tcctgggctg gtgatggcaa ggtacagctc
     1021 aatggcagcg tatccgatga caacgtattt tgccttgacc gggattggcg gaaatatgaa
     1081 cataagctga gtgttgggga aaagcatccc gaaggccagc aatacaccat acacggcacc
     1141 tgaagctccc acgaccggta cgttcagtat cgagttgagc atacccatct ggttgtctgt
     1201 gaagtttttc ccctggctga cggcattacc gcccatgtca tatacatact gaagctgctc
     1261 aggtgagagg attcccatca acttgttgta ctgtatccac tgcaccagct cgaagaagaa
     1321 ggctgcgccg aggcctgtca ccaggtaata caggaggaac cgcttcggtc cccaaacctg
     1381 ctcgagtatc ctcccgaaca tccacagcgc aaacatattg aaaagcagat ggacaaatcc
     1441 gccgtgcata aacatgtgtg tcactatctg ccatgagcgg aagaactctg atttcgggaa
     1501 gtaaagcccc aggatgccgt tcaggttgat gcctgaagtc agcctgagca gttcattaag
     1561 caggagcatc accacattga tgatgatgag gtttttcacc accgggggag tccctgaaag
     1621 tattctgttc atcttgttgt tctcacgtgt ttcagaacgc aaatttagcc attctgaccg
     1681 aatatcagct gaatctccgg tcaagctcct cgagggtgat gatgttcatc accgttttac
     1741 ctgagggaga gtagtttggc aatgcgcagg caaagagcat gtcaaagagt tcttccatct
     1801 ctgtgtgcgt cagcggggtg ccgtactgga tggctgaagc cctggccatg gagagggcaa
     1861 tccgttcgcg tgcacctatg gccgggtctc ccatcgaatg cttgtattca gcaatgattg
     1921 tttcgagcat cgacagtggg tttgcattgc ggcttgcggc ggggtggccg gtgatggtta
     1981 ccctgccatt cttcccggct tttatctcaa atccgagcat cctgagctgg tcgcctatct
     2041 cttcaataat aaccatgtcg ccagggttta gttccgcctc caccgggaaa agtgatacct
     2101 gtgcaggttt cgggccatcg cccagggagg taaggtattt ctcgtagagg actctctcat
     2161 gggcccttct ctggtcaatc atcattatgc cggagatcac ggggcacatt atgtagcggt
     2221 tcttcacctg gaagaaacgg cgctggcctg tcgcctgtcc tgaatccgcc gaccgaccgg
     2281 tagacatatc atctccgctc tcgctctcac tcttgtttcc gctgcgtgcg gtgatggtga
     2341 acagcgactc ccagtcgcgg ggcagattgc gttcggcatc ccatttccat ttcgtctcag
     2401 gtctctggta ttcggttccc tcgaagggat tgaaggtgtg gtcaacctct atctgcggca
     2461 gcggtggcgg aggagttttg cccatgatga ccggaatctc cacagcgccc tcgggtccga
     2521 agtcgatcga cggaacaacg ttgaaccggc ccagcgcctc gcggacggat gccagcatga
     2581 tctgccagac agacctctca tcctcgaact tgacctcggt tttggtgggg tgaatgttga
     2641 cgtctattgt tgcaggatcg gcggtcatgt agatgaagta tgccggcagc gtgtcaggag
     2701 gaaggagacc ctggtaagcc tccagcacgg cacggtgaag gtagggatgc cggatgaacc
     2761 tgttatttac aaagaagaac tgttcgccgg cggagcggcg ggcgttttcg ggcttgcccg
     2821 cgaaaccgct caccgtcact atggaggtgg aggtttccac acttaccagg ttctggttgt
     2881 aatgtttacc gaacagtcct gcaatgcgct gacgatggtt tcccgccggc aggcgatata
     2941 tctcctggtc attgtgggta aggttgaacc gtatctccgg gtgggccagc gccacacgct
     3001 ggaactcggt gatgatatgc cggaactcgg ttgtgtccga cttcaggaat ttgcggcggg
     3061 cggggacatt gtaaaagagg ttcctgacgc tgaacactga tcctgcggcg catgagcatg
     3121 gttcctgtct tgtgactctc gagttgttta tctcaaccag tgttcctgct tcgtcttctt
     3181 cgcgccttgt gcgcaactcg gtcattgaga cggctgctat tgaggcgagc gcctcgccgc
     3241 ggaacccttt ggtagttatg ataaagaggt catcggctga tgagatcttc gaggtggcgt
     3301 gtctctcgaa tgagaggcgg gcgtcggtct cgctcatgcc gcagccgttg tcaatcacct
     3361 gcaccagggt tcgtcccgca tcgcggataa cgacgcttat ctccgtggct cctgcgtcaa
     3421 cggcgttctc agtcagctcc ttgaccaccg aggccgggcg ctggatgact tcgcctgctg
     3481 caatctggtt agcgacggag tcgggcagga gctttatgat gtcactcatc cggtgcgatt
     3541 aaaaacggag gtaaagatag actacaaacg ccagaataat aattatcaca atcagcctga
     3601 tggtggtctg tttctgtact ttttcctgtc tgcggttaaa gtagtttttc atgtcgccac
     3661 gcatgatcct cgagcggtag ggctgacggg ctccctcttc cgtagcttca gtgaaggatg
     3721 actcactgtt tttttgttct tccccgcctt ttctctcctc ttccgcccat gctctgcgtc
     3781 tctgttccag ttcctcctta accgggtcat agtaccgggg tctgtagttg aagccgcggg
     3841 gtttgttctg tttgaagaaa ctcgggatag ccattgtgtt ctttttgcaa aaatagcaag
     3901 aaaacggtta gccgggaaga tccggccggt ttcccggagc taaagattgt tcacttttta
     3961 ttacttttgg aaggctaaac cttgttctag agcgatgaag attcttgtaa cccatgcggg
     4021 gacatccggg ctgtcagcca gtgtggctga gcgcgtggcc agggtattga ccggcctggg
     4081 agctgaggta actgtcaggc cgatgagtgg tgtatcgacg atcaaaggtt tttcagctgt
     4141 gattgccggt tgtcaggagc aggggggagg ctgggtaccg gaggccatgg agtttgtcag
     4201 gcgaaaccag tcggcattag gtaagaagcc gtttgccata ttcacagtca ttccgtcgct
     4261 gggtacggat aaaggtgccg agtcgaggat ggtgatcatg cagtatacgg ctcatgtgag
     4321 aggtatggtt catactgtga gtgaagggtt tttcagtggt gaaactgaaa accggggggc
     4381 accgcagccc ggtgagcggt cgaagttcaa gctgcggttg ttgctcgggt tgcttcgtga
     4441 ggtcgagacc agaagtaatt cggagatcga gacgtggtca gcccaggtgc atgaaaagtt
     4501 ggtcaagctg cttgccaaat aggcagatca gcattgagga tttgcgattt gcgattgacg
     4561 atttaggccg caggcctttc ggcattgtat ctcctggccg gctccccgcg ctcctatttc
     4621 acccttatat atttttcgga ccagtaattg cttttgtcca cctcccccgt ctcgtcggca
     4681 ggccaaacct ggatcagggt atcgtttgca acaacgattc tcattctcag ggtttctccg
     4741 atgtaatcat cagtgacgtg gtattttatg gtctccttgt agactattcc ctcgagtgta
     4801 taggatccgc cgccgtagct ttctgatatt atggtatcct gtttatactg tccgacgaac
     4861 atccagtagt tattgcacca cattttcacc tggccaccct ggtaagagtc cgggaatgtg
     4921 gctatgaccg aatcatttac cacctgtttg gcatagat
//
LOCUS       DAOPXU010000155         4926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_21765_length_4926_cov_10.414494, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000155 DAOPXU010000000
VERSION     DAOPXU010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21765_length_4926_cov_10.414494"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PKK03_11505"
     CDS             complement(<1..688)
                     /locus_tag="PKK03_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNX85066.1"
                     /translation="MKEVLEKVRKELREASDEKTRQTGLRYFKEEVNLYGIRSKTVSE
                     IARTNYALIRKAGKDEILLLCDSLWKSGMMEESFVACVWSEKMASEFVSADFAILEKW
                     VHNYVSNWASCDTLCNHTVGDFIQKYPEYLAELKKWARSENRWVKRASAVSLIIPARK
                     GKFLDDIFEIAGILLLDRDDMVQKGYGWILKAASQAHQEEVFNYVMQHKAVMPRTALR
                     YAIEKMPPALR"
     gene            complement(693..2411)
                     /locus_tag="PKK03_11510"
     CDS             complement(693..2411)
                     /locus_tag="PKK03_11510"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HNX85067.1"
                     /translation="MTRDRTGISDKWIRASIIGTIWAAAEIVLGSFLHNLRVPFSGNM
                     LTAIALVILIAVSYRWRQHGLYWRAGLICALMKAMSPSAIIFGPMIAIIMESVLLEVS
                     VRLLGRTYLGFIVGSMLAMSWNLFQKVFNMIIFYGGNLVEIYSGITAWAEKQLSLNFN
                     AFWAPLVLLLSIYALFGAVTAVAGIITGRRIASLPPEESLPEPAVIRAQEKKKKNSFD
                     YSLPWLAADILLVAAMLVAVSALSWVWWVIVVIPGVSVMAMRYKRAMRQLSKPGFWVT
                     FVVITMLSALVFTAISHEKNSLREAVLIGIQMNFRAVIIIVGFSALGTELYNPKIRTL
                     FSRTRFRQLPLALELSAASLPTMIADMPDLKTAVKTPVSILNRMITRVERRLNEVRKE
                     QEEERKVFIITGAIGEGKTAWLVKLTGLLRERGVMTGGILALRIMEEGKTTGYDITDI
                     RTGDRTPFLRQTGDAETGVERFTMDEAGHRAGQRALDPALNRSVDVVIVDEAGPLELR
                     GEGWSERITGILNETRAIIILAVRKNLIQDVTETFGISPTGITDVANGDVVRFADEIT
                     ALTIKR"
     gene            complement(2440..3378)
                     /locus_tag="PKK03_11515"
     CDS             complement(2440..3378)
                     /locus_tag="PKK03_11515"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNX85068.1"
                     /translation="MESRSRRSFLRGTAAAGAALFAAPSILSGKSRSAAVPDADKPFR
                     LAYAPSIGMFRELCGSEDPLDNIQFLADKGFRALFDNGFPGRPVEEQERISTKIQKMG
                     LMMGPWVLHADFGNTSFVMPHRDVRDFLTTKMEEGIEVARRTGFKTALVVPGRYDERL
                     HPDIQLANVIDNLRYCADLVGPEGMTLVLEPLNTLHDHPGLWLTRMPQAYQVCRAVGS
                     PAVKIVEDMYHQQITEGNLIPNIEACWDEINAFHVGDNPGRNEPTTGEINYKNIFSYL
                     LSRDYQGVICMEHGRSVRGPEGARRVIEAYRECDPF"
     gene            complement(3501..4829)
                     /locus_tag="PKK03_11520"
     CDS             complement(3501..4829)
                     /locus_tag="PKK03_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX85069.1"
                     /translation="MKKSSISRRNFLGTAAAAGAAGIVVPAFITSCSTEKRAKVVLPT
                     MLDAAPDGPLLKAGLIGCGGRGTGAALNFLHAGPNLTIAALGDVFADRITACRERLAK
                     DGVEIADEKCYTGFDAYQKVIDSGVDILILATPPYFRPMHFEAAVTAGKHVFMEKPVA
                     VDPAGARQIMATGEKAKGMELCVVTGTQRHHQHDYVATWGKVQEGMIGDIVAANCYWN
                     QNQLWYRDPNPSWSEMEYMIRDWVNWCWLSGDHIVEQHVHNIDVINWFTGKKPVSAVS
                     FGARHRRVTGDQYDFFSTDFIYDGGFHVHSMCRQINGCEGNVSEWIQGTKGTSNCQNT
                     VWDQAGTELWKYDGYKLDDQGKMTEELIRSPYDQEHIDFVTAIRTGKPLNEAEFVAVS
                     TMAGIMGRVSAYTGKKVTWDEMMNSELKLGPKVFEMGPVDVSKTVPVPGE"
BASE COUNT         1088 a         1411 c         1186 g         1241 t
ORIGIN      
        1 tccggagcgc gggaggcatc ttctcaatgg catacctcag tgccgtacgt ggcataactg
       61 ccttatgctg catcacatag ttgaatacct cttcctgatg cgcctggctg gcagccttca
      121 gtatccagcc gtatcccttc tgcaccatgt catccctgtc aaggagcaga ataccggcaa
      181 tctcaaagat gtcatcaaga aatttcccct tccgggccgg tattataagt gaaacagcgg
      241 aagccctttt tacccatcgg ttttctgacc ttgcccactt tttaagctct gcgaggtatt
      301 ccgggtattt ctggatgaaa tcgccaaccg tgtgattgca cagcgtgtcg caggatgccc
      361 agttactgac ataattgtga acccacttct caaggatggc aaaatcagca gatacaaact
      421 ccgatgccat cttctccgac catacacatg ccacgaacga ctcctccatc atgcctgatt
      481 tccacagtga atcgcacagc aagagaattt catccttgcc ggctttcctt atcagggcat
      541 aattcgttct tgcgatctca gagacagttt tggatcttat tccgtaaagg ttcacttctt
      601 ctttaaaata ccgcaaaccg gtctgacggg tcttttcatc tgaagcttcc ctgagctcct
      661 ttcttacttt ttccagtacc tctttcattg catcaccgtt ttattgtcaa cgcggtaatc
      721 tcatccgcga agcggacgac atccccgttt gccacatccg ttatccctgt cgggcttatc
      781 ccgaatgttt cagtaacatc ctgaatgaga ttttttctga ccgcaaggat aattattgcc
      841 ctggtttcat taagaatccc ggtgatcctt tcgctccatc cctctcctct cagttcaagc
      901 ggccctgcct catccacaat aacaacatcc acagacctgt tcaatgcggg gtcaagagcc
      961 ctttgtccgg ctctgtgccc ggcctcatcc attgtgaagc gttccactcc cgtctcagca
     1021 tcacctgtct gcctcaggaa gggagtcctg tcgccggtcc ggatatctgt tatatcataa
     1081 cctgtagtct tcccctcctc catgattcgc aatgcgagga tgccacccgt catgacacct
     1141 ctttccctga gcagccccgt cagtttgacc agccaggccg tctttccttc gccgatggcg
     1201 ccggttataa taaagacctt cctttcctcc tcctgctcct ttctgacctc attcagcctc
     1261 ctttcgacac gtgttatcat ccggttgaga atggaaaccg gtgtctttac cgccgtcttc
     1321 aggtcaggca tgtcagcgat cattgttggc aggctggcgg ctgacaattc gagggccagg
     1381 ggaagttgcc tgaagcgtgt gcggctgaac agagtgcgga ttttcgggtt gtaaagctcc
     1441 gtgcccagcg ctgaaaagcc tacaattatt atcacagccc ggaagttcat ctggatacca
     1501 ataagcactg cctcccggag gctgtttttt tcatggctga ttgcggtgaa gaccagggct
     1561 gaaagcatgg tgataaccac gaaggtgacc cagaagccgg gcttcgacag ctgtcgcatc
     1621 gccctcttgt acctcatggc cattacggaa acacccggaa tcacaactat gacccaccat
     1681 acccatgata gggcgctcac tgccacgagc atcgcggcaa ccaacagaat gtctgccgcc
     1741 agccatggaa gcgaatagtc aaacgaattt tttttcttct tctcctgtgc gcggatcaca
     1801 gccggctcgg ggagcgactc ctcaggcgga agggacgcta tccggcgtcc ggttatgatt
     1861 cccgcgacag cggtgacagc cccgaaaagg gcatagattg aaagcaacag taccagcggg
     1921 gcccagaagg cgttgaaatt cagactcagc tgcttctcag cccaggcagt tatgcctgaa
     1981 tatatctcca cgagattgcc cccgtagaaa atgatcatat tgaacacctt ctggaacagg
     2041 ttccatgaca tggcaagcat tgaaccgaca ataaaaccca ggtaagtgcg gccaagaagc
     2101 ctgacagaga cctcaaggag aaccgattcc atgatgatag caatcatcgg cccgaagatg
     2161 attgcgctgg gcgacattgc cttcatgagg gcgcatatca gcccggcacg ccagtagaga
     2221 ccatgctggc gccagcggta actgaccgca atgagtatga caagggctat tgcggtaagc
     2281 atgttaccgc tgaacggaac acgaaggtta tgcaggaaac tgccgagaac gatctcggca
     2341 gcagcccata tggtgccgat aatggatgcc ctgatccact tatcgctgat acctgtcctg
     2401 tcccttgtca aatgatgtag tgcagtttcc cttactcaat cagaacgggt cacactcacg
     2461 gtatgcctct atcacccggc gggcaccttc agggcccctt acactgcggc cgtgttccat
     2521 gcagatcaca ccctgataat cacgtgagag caggtaactg aaaatgttct tgtagttgat
     2581 ctcccccgtt gtaggctcat tgcggcccgg attgtctccg acatggaatg catttatctc
     2641 atcccagcag gcttcgatat tcggaatcag attcccctca gtgatctgct gatggtacat
     2701 gtcttcaact attttaactg ccgggctgcc caccgccctg cacacctggt atgcctgcgg
     2761 catgcgcgtc agccagaggc ccggatgatc atgcagcgtg ttcagcggtt ctagcacaag
     2821 tgtcattcct tcggggccta ccaggtcggc acagtacctg agattatcaa ttacatttgc
     2881 aagctggatg tcagggtgca gccgttcatc atacctgccg ggaaccacca gcgctgtctt
     2941 gaaaccggta cgcctggcca cctcaatgcc ctcttccatc tttgttgtca ggaagtcacg
     3001 cacatcgcgg tgtggcatca caaacgaagt gtttccgaag tcggcatgca gcacccatgg
     3061 acccatcatc aatcccattt tctggatttt tgttgatatc ctttcctgct cttccaccgg
     3121 acggccggga aaaccgttgt caaacagcgc cctgaatccc ttatcagcca ggaactggat
     3181 attgtcgaga gggtcttcac ttccgcacag ctcacggaac atcccgatcg agggagcata
     3241 ggccagcctg aacggtttat ccgcgtcagg aacagctgct gaacggctct ttccggaaag
     3301 aattgaaggg gcggcaaaga gtgccgcgcc tgcagctgcc gtccccctga gaaaactgcg
     3361 tcttgatctt gattccataa tacaatcagg tatctgtttg cccggcagat ctgactttgc
     3421 actgcagatc tgccggggtg tgattatcta ttcgcccggc acaggcacaa tcaagcgtac
     3481 cccgcccgga ccctcattct ttattcccct ggcaccggta ccgttttcga tacatccacc
     3541 ggtcccattt caaatacctt cggacccagt ttgagctctg agttcatcat ctcatcccat
     3601 gtgaccttct taccagtata tgctgagact ctgcccatga tgccggccat ggtcgacact
     3661 gcgacaaatt cagcttcatt cagcggcttc ccggtacgga ttgccgtcac gaagtcaatg
     3721 tgctcctggt cataagggct gcggatgagc tcttctgtca tcttgccctg gtcatcaagc
     3781 ttgtaaccat catatttcca gagctctgtt ccggcctgat cccataccgt gttctggcag
     3841 ttggatgtgc ccttcgttcc ctgtatccac tccgagacgt taccctcgca gccgttgatc
     3901 tggcggcaca tgctgtgcac atggaagccg ccgtcataga tgaaatcagt gctgaagaaa
     3961 tcatactgat cacctgtgac gcgacggtgg cgtgcgccga aggagacggc cgaaaccggc
     4021 ttcttgcctg tgaaccagtt tattacgtct atgttgtgga catgctgttc aacaatatga
     4081 tcacctgaaa gccagcacca gttgacccag tcacggatca tatactccat ctccgaccat
     4141 gagggattgg ggtcccggta ccagagttgg ttctggttcc agtagcagtt ggctgccacg
     4201 atgtctccta tcattccctc ctgtaccttg ccccatgttg ccacataatc gtgctggtga
     4261 tgacgctggg ttccggtaac aacgcagagc tccattcctt tcgccttttc gcccgtggcc
     4321 atgatctgcc ttgctcctgc cggatcgacg gcaacgggtt tctccatgaa cacatgcttg
     4381 ccagccgtta cggctgcctc gaaatgcatc gggcggaagt atggcggtgt cgcaagtatt
     4441 agaatgtcaa cccctgaatc aattaccttc tggtaggcat cgaaacctgt atagcatttt
     4501 tcatcagcaa tttccacccc gtctttggcc aggcgttcgc ggcatgcggt gatcctgtcg
     4561 gcaaagacat caccgagggc agctattgtc aggtttggtc ccgcatgtaa aaagttaaga
     4621 gcagcgcccg tgcctctgcc gccgcagcca atcaggcctg ccttgaggag cggaccgtca
     4681 ggtgctgcat caagcattgt tggcagcacg acttttgcgc gtttttcggt tgaacatgat
     4741 gtgatgaatg ccggcacaac gatgcctgcc gcccccgcgg ctgcagccgt gccaagaaag
     4801 tttcttctgg atatggatga ttttttcatg gtatgaattg gttggatagc caaagataat
     4861 gtttttgtct gaggtctgaa ggtctgaggt ctgaaggtct gaggtctgaa ggtctgaggt
     4921 ctgaag
//
LOCUS       DAOPXU010000156         4889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_21995_length_4889_cov_10.623707, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000156 DAOPXU010000000
VERSION     DAOPXU010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21995_length_4889_cov_10.623707"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PKK03_11525"
     CDS             complement(<1..695)
                     /locus_tag="PKK03_11525"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HNX85070.1"
                     /translation="MIKRFVFAAILALQSVAMISQPRHITVASDGTGEFTSVQEAVNS
                     IRAYMSDTTFMHIKAGVYREKVVIPSWVTNLFMKGEGAEKCVITWDDYAGLRNMGTFR
                     TYTILIQGSGFTAEDITFENSAGPVGQAVAVHADGDRAVFRRCRMLGNQDTLLTANQE
                     SRQYYEECYIEGTTDFIFGPATAWFERCHIHSKKNSYVTAASTVENQESGYVFNRCRL
                     TAADGIERVYLGRP"
     gene            781..2037
                     /locus_tag="PKK03_11530"
     CDS             781..2037
                     /locus_tag="PKK03_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HNX85071.1"
                     /translation="MKDPGKYSFGIGDRFSHQGKAQLEALMNAASKLGVEITPVWNKS
                     NREHNIVHSEPADTRAEADASVKAMNYNGPYFVDADHINLNTVDRFIPHSDFFTLDVA
                     DYIGKKASDTDIEGFVEAHRTFIGELRIPGIGGRIIVTESYLREFAGKFLFAVNEAAA
                     IYSHIASIKGEGTFVTEVSMDEVDQPQKPIDLLFILKGLADLKVPVQTIAPKFTGRFN
                     KGVDYEGDPERFGTEFEEDILVIKYAVRQFGLPAGLKLSVHSGSDKFSIYPIIGNLIR
                     KHDCGLHLKTAGTTWLEEVIGLALSGGSGLGLARQIYRNAYERRDELCGPYANVIDIK
                     PNLLPHPDEVDSWETLKFAATLRHVPGDPDYNPSFRQLIHVGYKVAAELGNSYTDALR
                     ENEQIISTCVWENIYRRHIIRLFTTD"
     gene            2048..3454
                     /gene="uxaC"
                     /locus_tag="PKK03_11535"
     CDS             2048..3454
                     /gene="uxaC"
                     /locus_tag="PKK03_11535"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HNX85072.1"
                     /translation="MKEYINEDFLLRGETARRLYHEHSEGFPIIDYHCHLSPEAIATD
                     RRFEDLGQLWLEGDHYKWRAMRTNGIDEKYITGKNSTFHEKYDRWAETVPMTMRNPLY
                     AWTHLELKRVFGVGRLLNPDTAAAIWEECTALLQQPEYSARGLMKKFNVEVVCTTDDP
                     VDSLEHHKTIRDQGFEIKVLPAWRPDKAMSPEDPAKFINYIYKLEETSGIEIKGFASL
                     IEALRVRHAYFADAGCVISDHGIEQFHADDYTEKEVGYILRKVLSGSCLESSEISKFR
                     SAMLYELAVMDSERGWVQQYHYGAIRNNNSRMFNQLGPDTGYDSIGDFTVARTLSKFL
                     DRLDAGGNLAKTILYNLNPRDNELIATMIGNFQDGSVPGKIQFGAGWWFLDQETGMIN
                     QLNTLSLMGLLSRFVGMLTDSRSFVSYPRHEYFRRILCNMLGDDIEKGKLPESEIGTI
                     GKMVEMISYYNAKNYFGF"
     gene            3478..4158
                     /locus_tag="PKK03_11540"
     CDS             3478..4158
                     /locus_tag="PKK03_11540"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HNX85073.1"
                     /translation="MSGFKRINVALGMEETGLVPLFYHRDPQVCREVIAACHDGGARV
                     FEFTNRGDFAHEVFSDVRKWALNELPDMMIGAGSIPDAPTAAIFMQMGADFVVSPSLN
                     PEVARICNRRKVLWIPGCGTMTEISQAEELGAEIVKIFPGTAVGGPSFVKAVMGPCPW
                     TSIMPTGGVEPTEENLAGWFGAGVTCVGMGSALVTDKIVEEGDWKTLEKTVRNTLNLI
                     GRLRTTRS"
     gene            4251..>4889
                     /locus_tag="PKK03_11545"
     CDS             4251..>4889
                     /locus_tag="PKK03_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008777414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX85074.1"
                     /translation="MTNYRWAITSLLFFSVAINYMDRQVLSLTWKDFIAPEFHWTNND
                     YGTITGLFSIFYAVCLLFAGRFVDWIDTKKGFLWAIGVWSAGAVLHAFCGIATSGITA
                     GKWLVGFEEARRIIGTVDDIGLVTSVSVTLFIFARFVLALGEAGNFPAAIKTTAEFFP
                     KKDRAFSTSIFNSGAQVGALIAPITVPVIAAKYGWEMAFIVIGAIGFIWMGFW"
BASE COUNT         1284 a         1212 c         1271 g         1122 t
ORIGIN      
        1 ggcctgccaa ggtataccct ttcaatgccg tcggcagcag tgagccggca ccggttgaaa
       61 acatatccgg attcctggtt ctcaaccgtt gaggcggcag ttacatatga atttttcttg
      121 ctgtgaatat gacatctttc aaaccaggct gttgcaggac cgaagataaa gtcggtagtg
      181 ccctctatgt agcactcctc gtaatactgc cggctctcct ggttggcagt gagaagcgtg
      241 tcctggttgc ccagcatcct gcagcggcgg aaaaccgccc tgtctccgtc agcatggacc
      301 gccaccgcct gccctaccgg accggcgctg ttctcaaagg tgatatcctc ggcagtgaat
      361 cccgaaccct gaatcaggat agtataggta cggaaggtcc ccatattgcg caaaccggca
      421 taatcatccc aggtgataac acacttttcc gcgccctcac ccttcatgaa aaggttggta
      481 acccacgacg gaatgacaac cttctccctg taaacaccgg ccttgatatg cataaaggta
      541 gtgtcagaca tataagccct tatcgagttt acagcctcct gtacggaggt gaattcaccg
      601 gtgccgtcag aggccactgt gatgtgtctg ggctggctga tcatagcaac cgattgcaat
      661 gccagtattg ctgcaaatac gaaccttttt atcatgtgtg aattattggt gttttgagac
      721 tctaatttag ttttttattg ctatttttcc gacaaagctg aaaaccaata caagataaat
      781 atgaaagatc cgggaaagta ctcgttcggg ataggtgaca ggttctcgca ccagggaaaa
      841 gcacagctag aagcgctgat gaatgcagca tcaaaactgg gtgttgagat cactcccgtt
      901 tggaataaat caaacaggga acacaacatt gtacattcag agccggctga cacccgtgcc
      961 gaggcagatg cttccgtcaa agccatgaat tacaacggac cgtactttgt cgatgccgat
     1021 catatcaacc tcaatactgt tgaccggttc attcctcact cagacttttt tacgctcgac
     1081 gttgctgact atatagggaa gaaagcatca gacaccgata tcgagggctt cgttgaagct
     1141 catcgcacgt tcatcgggga actccggata ccaggtatcg ggggcaggat catcgtgact
     1201 gagagctatc tgagggaatt cgccgggaaa ttcctgtttg ccgttaatga ggctgcagcc
     1261 atctacagcc atattgcatc catcaaggga gaagggacgt tcgttaccga ggtatcgatg
     1321 gacgaagtag accagccaca gaaacccatt gacctgctgt tcatcctgaa aggcctggca
     1381 gatctgaaag tcccggtgca gaccatcgct ccgaaattca ccggccggtt caacaaggga
     1441 gtagattatg aaggtgaccc tgagaggttc ggaacggagt tcgaagagga tatcctggtt
     1501 atcaaatatg cagtcaggca gtttggtctt ccggccggcc tgaaactgag tgttcattcc
     1561 ggcagtgaca agttctcaat ctaccctatt atcggaaacc tcatccggaa gcacgactgc
     1621 ggcctgcatc tgaaaacagc cggcaccacc tggcttgagg aggtgatagg tcttgccctc
     1681 tcgggcggtt ccggactcgg ccttgccagg cagatatacc ggaacgccta cgaaagacgc
     1741 gatgagctct gtgggcctta tgcaaacgtc atagacatta agccgaacct tcttccccac
     1801 cctgatgagg ttgattcctg ggagactctt aagttcgctg ctaccctgag acatgtgcca
     1861 ggagaccctg attacaatcc aagcttcagg cagttgatac acgtaggata caaggtcgct
     1921 gccgaactgg ggaatagcta tacggacgcc ctcagggaaa atgaacagat aatctcaaca
     1981 tgtgtctggg aaaacatcta ccggcgccat atcattcgtc tctttacaac agattaaatt
     2041 catgccgatg aaagaataca taaatgaaga cttcctgctc cggggtgaga cagcccgcag
     2101 gctctatcac gaacactcgg aaggattccc catcattgac taccattgtc acctgagccc
     2161 tgaagcaatt gccaccgaca gacgtttcga agacctgggt cagctctggc tcgagggtga
     2221 ccattataag tggcgggcaa tgcgaaccaa cggtatagac gagaaataca tcacgggaaa
     2281 gaattctaca ttccacgaaa aatatgaccg gtgggcagag accgtgccga tgaccatgcg
     2341 taacccgctc tatgcatgga ctcacctgga gctgaagagg gtattcggcg ttgggaggct
     2401 cctgaaccct gatacggcag ccgcgatatg ggaagagtgc acagcccttc ttcagcagcc
     2461 ggaatattct gcacgcggac tgatgaaaaa attcaatgtc gaagtggtat gcacaaccga
     2521 tgatccggtt gacagcctcg aacatcataa gaccatcagg gaccagggct ttgagattaa
     2581 agtcctgcct gcatggcggc cagacaaagc aatgtctcct gaagacccgg caaaattcat
     2641 taattatatc tataagcttg aagagacttc ggggattgag ataaagggat ttgcttcact
     2701 gatagaagct ttgcgtgtca ggcacgccta cttcgctgat gcaggatgcg tcatcagtga
     2761 ccatgggatc gaacagtttc atgccgatga ttacactgaa aaggaggtgg ggtatatcct
     2821 ccggaaggtg ctctccggat catgcctgga aagcagtgag atatcaaagt tcaggtcggc
     2881 catgttgtat gaacttgcag taatggatag cgagagagga tgggtccagc aatatcacta
     2941 cggtgctatc cgcaacaaca acagccggat gttcaaccag ctgggccccg acaccggata
     3001 tgactctata ggtgatttca cggttgccag aacactttca aagttccttg acaggctgga
     3061 tgccggtgga aacctcgcca aaacgatact ctataacctc aatccgaggg acaatgagct
     3121 aattgccacc atgatcggca acttccagga cggctcagtc ccgggtaaga tccaattcgg
     3181 tgccgggtgg tggttccttg accaggaaac gggaatgatc aatcagctca ataccctgtc
     3241 cctgatgggc ctcctcagca ggttcgtcgg gatgctcacc gactcacgca gctttgtctc
     3301 ataccccagg catgagtatt tccgcaggat tctctgcaat atgctgggcg atgatattga
     3361 aaaaggcaaa cttcctgaat cagagatcgg gactataggc aaaatggtag agatgatctc
     3421 atattacaat gccaagaact atttcggttt ctgaaaccac caaaataaga aaagacaatg
     3481 tcaggtttta aacggataaa tgttgcactg gggatggaag agaccggtct cgtaccactc
     3541 ttctatcatc gtgatcctca ggtgtgcaga gaggtaatcg ctgcctgcca tgacggggga
     3601 gccagggtat ttgaattcac caacaggggc gacttcgcac atgaggtctt ttccgatgta
     3661 aggaaatggg ctctcaacga gctgccggac atgatgatcg gtgccggatc aattcctgac
     3721 gcccccacag ccgctatctt tatgcaaatg ggtgctgact tcgttgtctc cccgtcgctg
     3781 aatcccgagg tggccaggat atgcaacagg aggaaagtgc tctggatacc cggatgcgga
     3841 acgatgaccg agatttcaca ggccgaggag ctgggtgcgg aaatagtaaa aatattcccg
     3901 ggtaccgcgg ttggcggacc atcgttcgtc aaagccgtca tgggcccatg cccctggaca
     3961 agcatcatgc ccacaggggg agtggaaccg acggaagaaa accttgccgg atggttcggg
     4021 gcaggtgtga catgcgtcgg catgggatcg gccctggtga cggataagat tgttgaggaa
     4081 ggtgactgga aaaccctgga gaagacagtg cggaacacac tcaacctcat cggcagactg
     4141 cggacaaccc gaagctgaca ggacctgaca ggcagctctg accaagaata cgaacacttg
     4201 tattaaccct ttaaacaatc aatatgtcct taaaaacctc agaatccggg atgacaaact
     4261 acaggtgggc catcacctca ttactattct tttcagttgc aataaactac atggacaggc
     4321 aggtactgtc actaacctgg aaggatttta tagcgccgga atttcactgg accaacaatg
     4381 actacggaac catcaccggt cttttctcga ttttctatgc cgtctgcctg ctttttgcag
     4441 gaaggtttgt cgactggatt gacaccaaaa agggatttct ctgggcaata ggagtctggt
     4501 cagcaggagc tgtgcttcat gctttctgcg ggattgcaac ctctgggatt actgccggga
     4561 aatggttagt gggttttgag gaagcacgca ggataatagg cacggtggat gacatcgggc
     4621 tggtgacttc ggtcagtgtc actctcttca ttttcgcacg attcgtgctg gctctcgggg
     4681 aggcgggcaa cttcccggca gccattaaga caactgcgga gttcttcccc aagaaggacc
     4741 gtgcattctc aacaagcata ttcaattccg gggcacaggt aggcgccctt atagcaccca
     4801 taaccgtacc ggtcattgca gccaaatacg gctgggaaat ggcattcata gtcatcggtg
     4861 caattggatt catctggatg ggattctgg
//
LOCUS       DAOPXU010000157         4814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_22492_length_4814_cov_10.612734, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000157 DAOPXU010000000
VERSION     DAOPXU010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22492_length_4814_cov_10.612734"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(649..1221)
                     /gene="rsxA"
                     /locus_tag="PKK03_11550"
     CDS             complement(649..1221)
                     /gene="rsxA"
                     /locus_tag="PKK03_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNX85075.1"
                     /translation="MEFILLIISAIFVNNVVLAQFLGVCPFLGVSNKVSTALGMSGAV
                     VFVIVLATMVTYLIHTYVLIPLGITFMQTITFILVIAALVQMVEIILKKISQPLYQSL
                     GIFLPLITTNCAVLGVAILVIKKDYNLLEGVVYGAATAIGFGLALVILAGIREQMELA
                     DVPSGMKGVPISLVTAGILALAFMGFAGLV"
     gene            complement(1234..1815)
                     /locus_tag="PKK03_11555"
     CDS             complement(1234..1815)
                     /locus_tag="PKK03_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNX85076.1"
                     /translation="MSQMQNFTKGFLKENPILVLVLGTCPTLATTSSALNGMGMGLAT
                     TFVLIGSNVVISLMKNVIPDKVRIPAFIVVIATFVTIVDQVMQAYVPSLYETLGIFIP
                     LIVVNCIVLGRAEAYASKNTVWSSFVDGAGMGLGFTGALGVLGAVREILGSGAIAGFK
                     FIEGDGILVFILAPGAFIALGYLIAIVNRINKA"
     gene            complement(1812..2405)
                     /locus_tag="PKK03_11560"
     CDS             complement(1812..2405)
                     /locus_tag="PKK03_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HNX85077.1"
                     /translation="MAKMQSTLKNMVLSLTLISLGASAALGFVYEMTKDPIAAALLNK
                     KIEAIKQVVPEFTNNPNDEMFMLPTGEGDSLEVYPAKNGDQVVGYAIKSYTNNGFSGY
                     IGFMVGFTPDGAIYNVNVLEQKETPGLGTKMVPDGTPEKPEFKDQFKGKNPAEYQLKV
                     KKDGGPVDAITAATISSRAFCDGVQRAYNTFEKGGLK"
     gene            complement(2398..3399)
                     /locus_tag="PKK03_11565"
     CDS             complement(2398..3399)
                     /locus_tag="PKK03_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNX85078.1"
                     /translation="MSNILNVSPSPHQQSAETTRKLMLGVVIALAPALAASVWYFGLG
                     AIIVTLVSVVSCVAVEYLIQKFILKTKPSVSDGSAIVTGLLLAFNVPSNLPVHIIIIG
                     AIVSIGVAKMTFGGLGNNPFNPALVGRVFLLISFPVQMTSWPVPSGFNTGYTDAVTGA
                     TPLAIMSEGIKNGEAVSALMEKIPSHMQLFYGYMGGSMGEIAAVALILGGLYMIWRKI
                     ITWHIPVTILLTVVVFTGILWAVDPTKYADPLFHLLTGGIMLGAIFMATDYVTSPMTA
                     KGMIIYGIGIGVITVLIRTWGAYPEGVSFAILIMNSFVPLLNMYIKPKRFGKEVKHG"
     gene            complement(3480..4814)
                     /gene="rsxC"
                     /locus_tag="PKK03_11570"
     CDS             complement(3480..4814)
                     /gene="rsxC"
                     /locus_tag="PKK03_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNX85079.1"
                     /translation="MLKTFPKGGIHPPENKLTADRPIVILPVPSQVTVPVSQHIGAPA
                     VPVVEKGAAVKAGQLIAQAKGFVSTNIHSPVSGKVNKIDTVVDTTGYKQTAVVIDAEG
                     DEWVETIDRSAALVKEITLSREQIIERCQQCGLVGMGGATFPTHIKLTVPAGKKCDML
                     IINGVECEPYLTSDHRLMLEKGAEVLTGVSIIMKALGVENAVIGIEANKPDAISQLTE
                     LAKDFKGITVQPLKVKYPQGGEKQLIKACTGREVPSGGLPVDVGVVVQNVGTSFAVYE
                     AVQKNKPLFERVVTVTGKSVKNPGNFMVRVGTPVTALIEAAGGMPDDTGKVVNGGPMM
                     GKALAITDVPVTKGTSGIIMFPADASKRAEVMPCIRCGKCSSACAMGLEPWLISVLGQ
                     KAMYEKAEQEKITDCMECGACAYTCPAKRPLLDYIRLGKATVIRMARERVTK"
BASE COUNT         1220 a         1354 c         1138 g         1102 t
ORIGIN      
        1 gcaaagggta gcttatgagc aagtcatcga agtgtcaggc ggctggtgca aagggtagct
       61 tatgagcaag tcagcggagt gtcatgctgc tggtgccagg ggtagctcgt gagcaagtca
      121 gcataatgtc atgctcccga tgtgccggga ttcaatgcag gagtcattgc agttccatga
      181 cccgggttgg gccatcaccg gcagatgaca taataagaca acccaattgt gccgggattc
      241 aatgcaggag tcattgcagt tccatgaccc gggttgggcc atcaccggca gatgacataa
      301 taagacaacc caattgtgcc gggcgtaaaa gccaattgat tatcagtaaa ttaaaaatca
      361 ggtctttaag attgcttttg tacaggtata atactccgcc ccgtcagtca cattaatacc
      421 ttaccggaga aagtgcatcg gggtgacttt cactgactga cagaagatgt aaaatgcaaa
      481 catggaaatt tagtgaaaaa ttttaaccga aacaagtaaa ttgttcgaaa aaccttattt
      541 atccattggc cggatacaga attacgtcct ggcggaggcg tgacaggaac attttttcag
      601 gaaaatacat gtcccggaca gatcggggtc agcgtttgaa aaaccttgtt acaccagccc
      661 agcaaaaccc ataaaggcca gggcaagtat accggcagtg accagcgata tgggcacccc
      721 cttcatccct gaaggtacgt cggcaagctc catctgctca cgaataccgg ccagaatgac
      781 caacgccagc ccgaaaccaa tggctgtggc tgccccgtat acaacgcctt ccaaaaggtt
      841 gtagtccttc ttgatcacca ggatcgcaac accaagtaca gcacagttcg tggtgatcag
      901 gggaagaaat attcccagcg actggtacag cggctgactg atcttcttca gaatgatctc
      961 aaccatctgc actagcgcgg caatcaccaa aataaacgtg atcgtctgca tgaaggtgat
     1021 acccagcggt atcagcacat aggtgtgaat caggtatgtg accatagtgg caagcactat
     1081 cacaaaaaca actgccccac tcatcccaag agccgtgctt accttgttcg agactcccag
     1141 aaagggacaa acacccagaa actgggccag caccacgttg ttgacaaaaa ttgccgatat
     1201 gatgagaagt ataaattcca tttcctttcc tccctaagct ttgttaatcc tgttcacaat
     1261 ggcaatcaga tacccgaggg caatgaacgc acccggagca agaataaata ccaggatgcc
     1321 atcaccctca atgaacttga atcctgcaat ggcaccactg ccaagaattt cgcggactgc
     1381 cccaagcacc cccagggccc ctgtaaaacc gagccccatc ccggcaccgt caacaaacga
     1441 agaccagaca gtattcttgc tggcatacgc ctccgcacgt ccaaggacta tacagttcac
     1501 cacaatcagc ggaatgaata tccccagagt ctcataaagc gaaggaacat aagcctgcat
     1561 tacctggtca acaatcgtca caaaagtagc tatgaccaca ataaatgcgg gaatacgaac
     1621 cttgtcggga atcacattct tcatcagtga aatgaccaca tttgacccga tgagcacaaa
     1681 tgtggtcgcc agccccatcc ccataccgtt cagggcggaa gaggtggtag caagggtggg
     1741 acatgttccc agtacaagca ccaggatcgg gttctccttc agaaatccct ttgtgaaatt
     1801 ctgcatctgg ctcatttcag acctcctttt tcaaatgtgt tgtacgccct ctgaactccg
     1861 tcacagaaag cccttgaact gatagtggct gcggtgatgg catcaaccgg accgccgtcc
     1921 ttcttcacct tcagctggta ctcagcaggg ttcttcccct tgaactgatc cttgaactca
     1981 ggcttttccg gagtcccgtc aggaaccatc ttggtgccca gtcccggcgt ctccttctgc
     2041 tcgaggacgt tcacattgta tatcgcgccg tcaggcgtaa atcctaccat aaaaccgatg
     2101 taaccgctga atccgttgtt cgtgtatgat ttgatcgcat aacccacaac ctggtcaccg
     2161 ttctttgcag ggtaaacctc cagtgagtcg ccctcgccag taggcagcat gaacatctcg
     2221 tcgttcgggt tatttgtgaa ctcaggcacc acctgcttga tagcctcaat cttcttgttg
     2281 agcagtgctg cagctatcgg atcctttgtc atctcgtaaa caaatccgag tgcggctgaa
     2341 gcgccaagcg aaataagcgt cagcgagagc accatgtttt tcagggttga ttgcatctta
     2401 gccatgcttc acctccttcc cgaacctctt tggtttgatg tacatgttga gcagcggcac
     2461 gaaagagttc atgatcagga tcgcaaatga tacaccctcg ggatacgctc cccaggtcct
     2521 gatcagcaca gtaataacac ctatgcctat accgtatatt atcattccct tcgccgtcat
     2581 cggcgatgtt acataatcag tcgccatgaa tatcgcgcca agcattatac cacccgtcag
     2641 cagatggaag aggggatcag catatttcgt ggggtcaaca gcccagagga tgccggtgaa
     2701 tacaaccaca gtaagcagta tggttaccgg gatgtgccat gtaattatct tcctccagat
     2761 catgtacagc cccccgagaa tcaaagccac agccgcaatc tcacccatgg agcctcccat
     2821 gtaaccatag aagagctgca tgtgcgatgg gattttctcc atcagggccg aaacggcctc
     2881 gccattcttg attccctcac tcatgatcgc aaggggagtg gcgcctgtga ctgcatctgt
     2941 ataaccggtg ttgaaacccg atggcacagg ccagctggtc atctgtaccg ggaaggagat
     3001 gagcaggaag acccttccca ccagagccgg gttgaacgga ttgttcccca atccgccgaa
     3061 ggtcatcttt gccacaccta tcgagactat ggcaccgatg ataataatat gaaccgggag
     3121 gttcgaaggc acgttgaatg ccaggagaag ccccgtgaca atcgccgatc cgtctgatac
     3181 cgaaggcttt gtcttcagga tgaatttctg gatcaggtac tcaaccgcca cgcatgagac
     3241 taccgatacc agtgtgacga taatcgcccc caacccgaag taccagactg aggctgcaag
     3301 ggcaggagcc agggcaatca ccacgcccag catcagcttg cgcgtcgttt cggcgctctg
     3361 ctggtgcggt gacggggata catttaaaat gttgctcata tctaatgttt gctgtttcgt
     3421 aacacgttta tctcttcatg ccgtgccagg gaatgaggag aaaggaagat tcttgtttgc
     3481 tatttcgtaa ctcgttcacg ggccatcctt ataaccgtgg ccttgccaag cctgatatag
     3541 tcaagcaggg gtcttttcgc cgggcaggtg tatgcacatg ccccgcactc catgcaatcg
     3601 gtgatcttct cctgctctgc cttctcatac atcgccttct gcccgaggac cgaaatgagc
     3661 cacggctcga gacccatggc acaggccgag gaacactttc cgcacctgat acacggcatg
     3721 acctctgcac gcttcgatgc atcagcagga aacatgatga tccccgaggt ccccttcgtg
     3781 acaggtacat ctgtaattgc cagcgccttg cccatcatcg gaccgccgtt aaccaccttg
     3841 ccggtgtcat cgggcatgcc tcctgcagcc tcaatcagcg ccgtaacggg agtgccgacg
     3901 cgaaccatga aattaccggg gttctttacc gacttcccgg tgacggtcac caccctctca
     3961 aacagcggct tgttcttctg aactgcctcg taaacggcaa acgaagtccc tacattctgt
     4021 acaacaacac caacatcaac aggaagcccg ccggatggaa cctcacggcc ggtacaggcc
     4081 ttgatgagct gtttctcacc accctgcggg tacttgacct taaggggctg aacagtaatg
     4141 cctttgaaat cctttgcaag ttccgttaat tggctgatgg catctggctt gttcgcctca
     4201 atgcctatta ccgcgttttc aaccccgagg gctttcatga ttattgagac gcccgtcaac
     4261 acctcggcac ccttctcaag catcagcctg tggtccgaag tgagataagg ttcgcactcc
     4321 actccgttga taatcagcat gtcacacttc ttgccggcag gaacggtaag cttgatatgg
     4381 gtggggaagg tcgcacctcc catgcccaca agcccgcact gctgacacct ctctattatc
     4441 tgctcacgcg acagcgtgat ctccttgacc agtgcggcgc tgcggtcaat tgtctcaacc
     4501 cattcgtctc cctcggcatc aatcactacc gcagtctgct tataaccggt ggtgtcaaca
     4561 acggtgtcta tcttgttcac cttgccggaa accggcgaat ggatgttcgt ggagacaaac
     4621 cctttggcct gggcaattaa ctgcccggcc ttcactgcag cacccttctc aaccaccggc
     4681 actgcaggag ctccgatgtg ctgcgagacc ggcaccgtca cctgtgacgg aacgggaaga
     4741 ataactatcg gcctgtcagc cgtcagcttg ttctctggcg gatggatacc gcccttcgga
     4801 aatgtcttta acac
//
LOCUS       DAOPXU010000158         4778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_22771_length_4778_cov_10.775778, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000158 DAOPXU010000000
VERSION     DAOPXU010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22771_length_4778_cov_10.775778"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..537)
                     /locus_tag="PKK03_11575"
                     /pseudo
     CDS             complement(175..537)
                     /locus_tag="PKK03_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
     gene            complement(580..1314)
                     /locus_tag="PKK03_11580"
     CDS             complement(580..1314)
                     /locus_tag="PKK03_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HNX85080.1"
                     /translation="MGKFAKWIGGGLGFAIGGPLGGLLGFFIGSVFDGTTITYQQTGT
                     GRRINRTTPGDFGMSLLVLIAAVMKADGKVLKSELDYVKRFFISQFGPETAQEALQIL
                     RDLLKQEIPVRDVCMQIRSNMDYPSRLQLIHILYNISAADSRFEPKEVSVIKSIADML
                     GISSTDYDSIRNMFIPATDSAYRILEVERTATDEELKTAYRKMALKYHPDKVSHLGED
                     FRKTAEEKFKAVNEAWEKIKKERNIV"
     gene            complement(1314..1913)
                     /gene="coaE"
                     /locus_tag="PKK03_11585"
     CDS             complement(1314..1913)
                     /gene="coaE"
                     /locus_tag="PKK03_11585"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNX85081.1"
                     /translation="MAKRLGVTGGIGSGKTTVCRIFRVLGIPVFVADNVARDVMENEP
                     DIRAAINLITGKDLYTHGNLDRKELARIIFNRPDLLRKVNAAVHPEVLERFNRWASAS
                     DSPYVIMEAAILFEAGADSLVDRVVTISAPVEERISRVMGRNELTREEVIRRINNQLG
                     DEEREEQSYYVINNSDNEMIIPEILKIHEDMLRLAEKER"
     gene            complement(1930..2700)
                     /locus_tag="PKK03_11590"
     CDS             complement(1930..2700)
                     /locus_tag="PKK03_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HNX85082.1"
                     /translation="MNDKKERSERFLNRDLLVFAFFLLLSFIFWYLNELSKELQGSIN
                     YPVRYINPPKDRIVTGDLPDKLEMDLRGPGYSILKVKLSGSRAPVVIDFTKVAPRRIP
                     GQLPRYFLVTSGLMSNFSKQLHADFDILAIHPDTLFFGYDKLVTRKMPVIPNLKVELA
                     EGKKVIIVADPDSITVTGPQHVLDTLDGIPTKFRTFRRTNENFRSRIPIECPEYLQTA
                     QKRVTVEVTVAGRAASSQNGKHAADRRQTLTGEQRPGS"
     gene            complement(2725..3051)
                     /gene="yajC"
                     /locus_tag="PKK03_11595"
     CDS             complement(2725..3051)
                     /gene="yajC"
                     /locus_tag="PKK03_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNX85083.1"
                     /translation="MTTLASIYLQAAPAQGSPNLLTGLLPLVLVFVVFYFFMIRPQMK
                     KQKELTNYRNAITKGDKVITTGGIYGKVVDVTDNVVTVEIANDVRIRVDKNAILKDPS
                     DVAARQ"
     gene            complement(3100..3537)
                     /locus_tag="PKK03_11600"
     CDS             complement(3100..3537)
                     /locus_tag="PKK03_11600"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNX85084.1"
                     /translation="MKNALTYLVAVLVTAALLTGACGRNSSGAGNIAASDTTGTAVLT
                     FTAPEHNFGKVEAGEKVGCIFSYTNTGDADLVISSASASCGCTVPKYDKRPVPPGRSG
                     TIEVIFDTSGREGVQTKTVVVQSNAENNLVILRIIADVTKKGS"
     gene            complement(3550..4569)
                     /gene="nusB"
                     /locus_tag="PKK03_11605"
     CDS             complement(3550..4569)
                     /gene="nusB"
                     /locus_tag="PKK03_11605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNX85085.1"
                     /translation="MISRRQLRIKALAVLYSCNRKETADLETAEQELMLSIRQSYDLY
                     HYLLLLLISLSDLARERVALSGMKKVPGPEDINPNRRFADNRVIAQLRKNEQLRSYAE
                     ATPSLWTRKDKLNSMKALSRWLATKGLSWNDHPEVVRIIFNEMIQWDVYKAYMNSPES
                     SYKADLRFIKDVVTKFFPASEDLETSLEEQSVYWNDDLPFAASMIRNSLGRFRENDEG
                     SAISIPPLFTNDDDEKYVKVLLRKTILHSEKNSELIDAHTTNWEVERIALMDKLVMQL
                     AITELVEFPEVPVKVTLNEYIEIAKEYSTAKSSTFVNGILDKIVKEMRAGGTINKSGR
                     GLIGE"
BASE COUNT         1075 a         1247 c         1097 g         1359 t
ORIGIN      
        1 ttcggctttt gctgtcttgc ctgcttcggc ttttgctgtc ttgcctgctt cggcaccttt
       61 ttcctttttg ggtttggcgg tggtgccatt acctgccgtc tcttcactta cggactgacc
      121 ttccccttct tccttctcgg caggcttctc ctcctctttt accaggagat tcagcccctg
      181 aagtatgtta taccagtgaa gcaccttcct gatgtccgag gtatagaccc tttcgcggtc
      241 atactccggg agcgcctccg cgaagtattt cttaagctcg gcgtcaggct gcctgtgaga
      301 cagggccggt ccgccattct ccttttccca gatcatgtcc ataacctttg cgagtggtat
      361 atcctcgccg gttgtgtaaa tggaaatctc atccagtgcg cttaccttgg ccgtcgtacc
      421 ggcacttgac cgttttcccg tctcaaggtt ctcaacaatg actgcattct taccctgggc
      481 aatgaatttg aagagggtac cttcaccgga aatagccaga atatctttca ggttcatatt
      541 cggaatgatt aatgttaata actgtatttc aatcttttat cagacaatat ttcgttcttt
      601 cttgattttt tcccacgctt cgttcacagc cttgaatttc tcttcggccg tcttcctgaa
      661 atcctctccg agatggctga ctttgtcagg atgatatttc agggccattt ttctgtaggc
      721 agtcttcagc tcctcgtcag tggctgtccg ctcaacctca agaatccggt aagctgagtc
      781 agtggccgga atgaacatgt tgcgtatcga gtcgtaatcg gtagagctta taccgagcat
      841 gtcagcaatt gactttatga cactgacctc cttaggctcg aaccggctgt cagcggcgga
      901 gatgttatag aggatatgta tcagctgaag cctcgatgga tagtccatgt tgctccgtat
      961 ctgcatgcat acgtccctga cgggtatttc ctgcttcagc aggtcacgga gtatctgaag
     1021 cgcttcctgg gccgtttccg gtccgaactg tgagatgaag aaccttttga cataatcaag
     1081 ttctgacttc agaacctttc cgtcagcctt catcacggca gcgatcagca ccaggagact
     1141 catcccgaaa tcaccggggg ttgtacggtt gatccttcgt ccggttccgg tctgctgata
     1201 tgtgattgtg gttccgtcaa aaacggaacc gatgaaaaat cccagtaacc ctcccagcgg
     1261 tccgcctatg gcaaatccga gtcctccgcc tatccacttg gcaaacttac ccatcacctc
     1321 tccttttctg caagacggag catatcctca tggatcttga ggatctccgg tatgatcatt
     1381 tcgttgtcag agttgttgat gacatagtac gactgttcct ctctttcctc atccccgagc
     1441 tggttattga tcctgcggat cacctcttca cgggtaagct catttcttcc catcacccgg
     1501 gaaatgcgtt cctcgacagg tgcggaaatt gtaaccaccc tgtctaccag agaatcagct
     1561 ccagcttcaa agagaatggc agcctccatt ataacataag gggaatctga agccgaagcc
     1621 caccggttga accgctcaag cacctcagga tgcactgcgg cattcacctt gcggagaaga
     1681 tcaggcctgt tgaagatgat ccgggcaagc tccttgcggt caaggtttcc gtgagtatac
     1741 agatccttgc cggttatcag gtttatggct gcgcggatgt caggctcatt ttccatcaca
     1801 tccctcgcaa cattgtcagc aacgaacaca ggaatcccga gaaccctgaa gatcctgcat
     1861 acggttgtct tgccgctgcc tattccgcct gtgactccca gtcgttttgc catcagtagt
     1921 cctccttttt cagcttcccg gcctttgttc accggtgagc gtctggcgcc ggtcagccgc
     1981 gtgcttcccg ttttgggatg atgctgcccg ccctgctacc gtaacctcca ctgttaccct
     2041 cttctgggcg gtctgcagat attcagggca ttcaatcggg atcctcgacc tgaagttttc
     2101 attcgtccgc ctgaaggtcc tgaactttgt aggtatgccg tcaagggtat ccagcacatg
     2161 ctggggccca gtgacagtaa tgctgtcagg gtcagccaca attataacct tctttccttc
     2221 ggcaagttcc accttcagat tcggaatcac aggcatcttt ctcgtcacca gcttgtcata
     2281 accaaagaag agggtatcgg gatggatggc aaggatatcg aaatcagcat gcaactgctt
     2341 tgagaaattg ctcatgagcc ctgaggtaac aagaaagtag cggggcagct ggccgggaat
     2401 tcttcgcggt gcaacctttg taaagtcaat cactaccggt gccctgctgc ccgacaattt
     2461 caccttcagg atggagtagc ccggaccgcg gagatccatc tccagcttgt caggaagatc
     2521 tccggtcaca atccggtctt tgggcggatt gatatatctt accgggtaat ttattgagcc
     2581 ctgcaactcc ttgctgagct cgttaaggta ccagaagata aaggaaagga gcaggaaaaa
     2641 cgcaaaaaca aggaggtccc tgttcagaaa tctttccgat ctttcctttt tatcattcat
     2701 ggttgcaatg tttacactac tgttttattg cctggcagca acgtctgacg gatccttgag
     2761 gatggcgttc ttgtcaaccc tgatccttac gtcattggca atctctactg tcacaacgtt
     2821 gtcagttacg tccacaacct taccgtatat gccaccggtg gtaattacct tatcaccctt
     2881 ggtgatagca ttgcggtagt ttgtcagttc cttctgcttc ttcatctgcg gccttatcat
     2941 aaagaaatag aaaaccacaa ataccaggac aagcggaaga aggccggtta agaggttcgg
     3001 acttccctgg gcaggagctg cctgcagata aatggatgct aatgttgtca tgtgtgttgt
     3061 ctttgtttga tgttatttgt attgattaat atttacctgt taactgcctt tcttcgtcac
     3121 atcggcaata atacgcaaaa taactaaatt attctctgca tttgactgaa ctacaacagt
     3181 ttttgtctga actccctctc tcccggatgt atcaaagatg acctctatgg tgccggatcg
     3241 gccgggaggc acaggtcttt tgtcatattt tggcaccgtg caaccgcagc ttgcagaagc
     3301 tgagctgata acaaggtcag catcgccggt attggtgtat gagaagatgc atcccacctt
     3361 ctctcctgcc tccacctttc cgaagttgtg ctccggtgca gtgaaagtca gcacggccgt
     3421 gccagtggtg tctgaggctg caatgttgcc ggcccctgaa ctgttccttc cgcatgcgcc
     3481 ggtcagaagt gctgcagtca caagaactgc caccagatat gtcaaagcat ttttcattcc
     3541 ttaatccttc tattcgccta tgagccctct gccggacttg tttatagttc cgcccgccct
     3601 catctcctta acgatcttgt caagaattcc gttcacaaaa gtactgcttt tggcagtgct
     3661 gtactctttg gcaatttcaa tatattcatt cagtgtaacc tttacaggca cctccggaaa
     3721 ttcaaccaac tcagtaatag ctaattgcat caccagctta tccatcaggg ctatccgttc
     3781 cacttcccag ttggtggtat gggcatcaat cagctcactg tttttctcac tgtggaggat
     3841 tgtctttctg agcaatacct ttacatattt ttcatcatcg tcattggtaa agagcggagg
     3901 gatggatatc gcgcttccct cgtcattttc cctgaacctg cccagggagt tgcgtatcat
     3961 ggaagctgca aagggcaggt cgtcattcca gtagacagac tgctcctcga ggctggtttc
     4021 gaggtcctcc gaagccggga agaattttgt taccacgtcc ttaatgaagc gaaggtcagc
     4081 cttgtaactg ctttcgggag agttcatgta agccttgtag acgtcccatt gtatcatctc
     4141 attgaagatg atcctgacca cttccggatg atcattccac gaaaggcctt ttgtcgcgag
     4201 ccagcggctc agagccttca tgctgttgag cttgtccttg cgggtccaca gtgagggtgt
     4261 cgcttcggca taactgcgaa gctgttcatt cttcctcagc tgggcgataa ccctgttgtc
     4321 tgcaaacctt ctgtttggat taatgtcttc tggtccggga accttcttca tgcctgaaag
     4381 tgccaccctt tcccgggcca gatcagacag ggagatgagc agaagaagca gatagtgata
     4441 cagatcatag ctttgcctga tgcttaacat gagctcctgc tcggcagttt cgagatcggc
     4501 tgtctccttc ctgttgcagg aatagagcac agccagcgct ttgatcctga gttgtcttct
     4561 gcttatcact taaagaacat tttttcaggc agcaaagtta aaaaaatcaa catagcatgc
     4621 cacctccggg ccaaaaaagt atcacaattt gtcagcctga ctttgagttg aaaaaataat
     4681 tgttacattt gagcatgcta accgattgaa accctaaaac attcgctact gatggaagac
     4741 gaaaaaggaa gaattgaagg tcagaatgag agaagtga
//
LOCUS       DAOPXU010000159         4753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_22949_length_4753_cov_13.211367, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000159 DAOPXU010000000
VERSION     DAOPXU010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22949_length_4753_cov_13.211367"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PKK03_11610"
     CDS             complement(<1..872)
                     /locus_tag="PKK03_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNX85086.1"
                     /translation="MKYTGLSRQIWNNNIKSVVLLILFPIVILGLVWLFVFLVQPLQD
                     YRVDRANQLFFTSLPWVIAGVGLWFVIAWFSHTGMIERATGSAPLSRKENKRIYNLVE
                     NLCIASGMPMPKVNIINDDSLNAFASGINTKTFTVSLSRGIIEKLNDEELEAVIAHEL
                     THIRNRDVRLLIISIIFVGIFAFLTEALVRSVRFSGGSSGRKDGRAVAVALMLAAVGY
                     FLSSIFRFAISRQREYMADGGSAMMTKNPLALASALEKISVDSQIEAVKRNDVAQMFI
                     DNPQDKKVRPVSFS"
     gene            complement(877..1431)
                     /locus_tag="PKK03_11615"
     CDS             complement(877..1431)
                     /locus_tag="PKK03_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNX85087.1"
                     /translation="MFYALLIIIAVLALLAVSLYNRLVRLRNNRENAFADIDVQLKQR
                     LDLVPQLVEAVKAYMKHESTVLTEITKARASAIEAKGIDEKIAADGRLSSALQGLRVA
                     VEAYPDLKASTNFIQLQEEISDIENKLAAARRFFNSATKELNNAVETFPSNLIAGMFG
                     FKRETMYDLAETRAQAEETPKLNF"
     gene            complement(1510..2676)
                     /locus_tag="PKK03_11620"
     CDS             complement(1510..2676)
                     /locus_tag="PKK03_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HNX85088.1"
                     /translation="MTADSSRLHVVDALRGFAIVSIMLLHNIEHFDFYFLPEGLPAWM
                     VTLDKGIWDTLFFIFGGKSYAIFALLFGLTFFIQSDNQARRGFDFRGRFAWRLFLLLL
                     FGIINSSFYQGDILTIYAVIGLVLIPVARLGDRSVFWIALFLMFQPYEWFGIIKGLHN
                     PGAGLADPVSWSYFARTGEHITGDSLLKTWVGNLTIGKPAVLYWNWENGRVFQTASLF
                     MSGMLAGRRKLFVLSDSSRRFWKKTFLVASVTFIPLFIIKTHISGWIGSEALSRPAST
                     ILTSWSNMAFMLVLVSGFVLLYWNERSHRALNIFSNYGRMSLSNYMIQSVLGSFIYYG
                     YGLGLYKHTGATICLLIGILLAVLQGYFSSWWLKHHNKGPLETLWHYATWIGSK"
     gene            complement(2688..3341)
                     /locus_tag="PKK03_11625"
     CDS             complement(2688..3341)
                     /locus_tag="PKK03_11625"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNX85089.1"
                     /translation="MNDEKEVIREVITFVKQAMQEHESGHGWSHIERVVRMALHIRES
                     EGCGDRLTVELGALLHDVGDHKFRAHDGPAEIRKLLGRLGVGNKVTDEVVSINENISF
                     SKGKRPDSGSIAFRIVQDADRLDAIGAIGIARAFSYGGFRGNEIYDPHIKITDPPLLA
                     GASTKSVSTIHHFYDKLLLLRDLMNTPTGRRLAEERHDYMVKYLEQFFTEWNPGNDL"
     gene            3413..4657
                     /locus_tag="PKK03_11630"
     CDS             3413..4657
                     /locus_tag="PKK03_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HNX85090.1"
                     /translation="MNSAEIITIGDELLIGQTIDTNSAWMGMQLSMKGIRVNRITSIS
                     DSREGIIAALDESLRRVPLVLMTGGLGPTSDDITKETLAEYFGTKLVMDQGVLEEVTA
                     RIKRRNLEMNENNRRQAMVPESCRVLANHAGTAPAMLFEKEGRIIVSMPGVPSEMKQI
                     MQEHVLPMISAMAGGHTIIHRNIMTFGTFEAKLAERLEGFEKELPAMVKLAYLPAHGI
                     IKLRLTGTGDDPELVRMTEEEQVRKLYSIIPEVIYGEDEVTLEEIVGKLLLDNNLTVS
                     TAESCTGGKIASMITSVPGSSAWYRGSVVAYDNSIKTGVLGVDPGIISRYGAVSEETV
                     RAMAERMRSIAETDYAIAVTGIAGPTGGTPEKPAGTVWIAVASASGVVAEKHRFADDR
                     QVNISRSATNALNLLRKQIISR"
BASE COUNT         1228 a         1171 c         1217 g         1137 t
ORIGIN      
        1 attgaaaatg ataccggcct tactttcttg tcctgggggt tgtcaatgaa catctgggca
       61 acgtcatttc gctttaccgc ctcgatctgc gaatcgacag agatcttttc gagtgctgat
      121 gcgagtgcaa gcgggttctt tgtcatcatt gccgaaccgc catctgccat gtattcgcgc
      181 tggcgcgaaa tggcaaaacg gaatatcgac gagaggaagt acccgacggc ggcaagcatc
      241 agcgccacgg caacagcccg gccatccttt cttccgctgc tgcctccgct gaagcgaacc
      301 gaccggacca gtgcctcggt caggaaagca aagatcccga cgaaaataat ggaaatgatc
      361 aggagcctga catctcggtt acggatatga gtcagctcat gggcgatgac cgcctccagc
      421 tcttcgtcat tcagtttttc aattattccc cgcgagagcg agacagtgaa ggttttggtg
      481 ttgatgccgc tggcaaaggc attcagcgag tcatcgttga tgatattgac cttcggcatc
      541 ggcatgccgc tggcaataca caggttctcg accaggttgt aaatcctctt gttttccttc
      601 ctgctgaggg gagcggagcc ggttgccctt tcgatcatcc ccgtatggga aaaccatgca
      661 attacgaacc acagtccaac gcctgcaatc acccatggca atgaggtgaa aaagagctgg
      721 ttggcccggt caaccctgta gtcctgcaga ggctgcacca ggaagacaaa gagccacacc
      781 agtcccagta tgacaatcgg aaagagaatg agcaaaacga cagatttgat attattgttc
      841 cagatctgcc tgctcagccc ggtgtatttc attttttcag aagttcagtt ttggcgtctc
      901 ctcagcctgt gccctggtct cggccaggtc atacatggtt tcgcgcttga agccgaacat
      961 gcctgcaata aggtttgagg ggaatgtctc aacggcattg ttcagctcct tcgtggcgga
     1021 gttaaagaag cggcgtgctg cggcaagctt attctctatg tcagatattt cctcctgcaa
     1081 ctgaatgaag ttggtgcttg ccttcaggtc ggggtaagcc tccacggcaa ccctcagtcc
     1141 ctgcagggca gacgacaacc tgccgtcagc tgcaatcttc tcatcaattc cttttgcttc
     1201 gatggcgctg gcacgggctt tggtgatttc ggtgagcaca gtgctttcgt gcttcatgta
     1261 agccttcacc gcttcaacca gctgcggcac caggtcaagc ctctgtttca gctgtacatc
     1321 gatgtcagca aaggcattct ccctgttatt cctcaggcgt acgaggcggt tgtacaggct
     1381 cactgccagg agggccagaa ccgcgatgat aatcagcaat gcatagaaca tttctttagt
     1441 ttttagttgt tcacgtaaaa ataacacatt aaaacttatt ttaacaagtt ttcacggata
     1501 tggccggact tattttgaac ctatccatgt cgcatagtgc cacaatgttt cgagagggcc
     1561 cttgttgtga tgcttcagcc accatgaact gaaatatccc tgaagcacag cgagaagtat
     1621 tccaattaac agacatatgg ttgcgccggt gtgtttatac aatccgaggc cgtaaccata
     1681 ataaatgaat gaccccagta ccgactggat catatagttc gaaagactca tgcggccgta
     1741 gtttgagaag atgttcagcg ctctgtgcga cctttcattc cagtagagca atacaaatcc
     1801 cgaaacgagc accagcataa aggccatgtt tgaccaggag gtcaaaattg ttgatgccgg
     1861 gcggctcagg gcttcactgc caatccaccc ggaaatgtgt gttttaatga tgaagagggg
     1921 tatgaaagtc acagatgcaa ccaggaaagt ttttttccag aatctccgtg atgagtctga
     1981 caggacaaac agtttccttc tgcctgccag catgcctgac atgaagagtg atgcagtctg
     2041 gaagaccctg ccgttctccc agttccagta caggacagca ggttttccga ttgtcaggtt
     2101 acccacccag gtcttcagaa gggaatctcc tgtaatatgt tcacctgtcc tcgcgaaata
     2161 agaccatgat acgggatccg ccagaccggc tcccggattg tgtaaccctt ttattattcc
     2221 aaaccattca taaggctgga acatcagaaa caatgcaatc cagaagacac ttctgtcacc
     2281 cagccgggcc acaggtatga gtacaagccc gatcactgca tagattgtga gtatatcacc
     2341 ctggtaaaac gaagagttta tgatgccgaa tagcagaagc aggaataatc tccatgcaaa
     2401 tcttccgcgg aagtcgaagc ctctccttgc ctggttgtcg gactgaatga agaaggtcag
     2461 tccgaacagg agggcaaaga ttgcgtacga cttcccgccg aaaataaaga agagggtgtc
     2521 ccatatccct ttatcaagcg tgaccatcca ggcaggcagt ccttcaggca aaaaataaaa
     2581 atcgaagtgc tcaatgttat gaagcagcat tattgagaca atggcaaatc cacgcagtgc
     2641 atccaccaca tgaagacgtg aagaatcagc agtcatttgt tgagaggtta taaatcattc
     2701 cccggattcc attccgtgaa gaactgttcg aggtacttca ccatgtaatc atgtctttct
     2761 tccgcaagcc tgcggcctgt gggagtgttc ataaggtctc tcaggagcag gagtttatca
     2821 tagaagtgat ggatggtcga cactgatttt gtactggccc cggccagcag gggcggatca
     2881 gtaatcttta tgtgtggatc atatatttca ttccccctga acccaccgta actgaatgcc
     2941 ctggcaatgc cgatagcccc gatggcgtca agcctgtcag catcctgaac tatccggaac
     3001 gcgatgctgc cgctgtcagg tctcttccct tttgagaagg agatattctc attgatgctg
     3061 acaacttcat ctgtaacttt gtttccgaca ccaaggcgtc ccagcaactt tcgtatctcc
     3121 gcaggtccgt cgtgagccct gaacttatgg tcgcccacat cgtgcagaag tgcacccagt
     3181 tctacagtca gcctgtcacc gcatccctca ctttcgcgga tatgcaaagc cattctgaca
     3241 actctttcga tgtgagacca tccgtggccg ctttcatgct cctgcattgc ctgcttcacg
     3301 aaggtgatta cctccctgat gacttccttc tcatcattca ttagaaacta cataattagt
     3361 gacgcaaaat attgataatt ttgccaccct gatcaaaggt cacttacaaa atatgaacag
     3421 cgcagagatt atcacgatag gcgatgaact gctgataggg cagaccattg acaccaactc
     3481 ggcatggatg ggtatgcagc tcagtatgaa aggcatacgg gtgaaccgga ttacatcgat
     3541 ctctgacagc agggaaggga tcattgctgc tcttgacgaa tcgctgcgca gagtgccgct
     3601 cgtgttgatg acgggaggac tcggccctac aagtgatgat atcacgaaag agacactggc
     3661 tgagtatttc gggacgaagc ttgtcatgga ccagggtgtt cttgaagaag tcactgccag
     3721 gatcaagcgg aggaatctgg agatgaatga aaacaacagg cgccaggcga tggtgccaga
     3781 gagctgcaga gtacttgcaa accatgcggg tacagctccg gccatgctgt ttgagaagga
     3841 gggaaggatt atagtgtcaa tgcccggggt tccctccgag atgaagcaga tcatgcagga
     3901 gcatgtattg ccgatgatat cagccatggc cggcggtcat acaatcattc acaggaacat
     3961 tatgactttc ggcactttcg aggcgaaact ggccgagagg ctggagggtt tcgagaagga
     4021 actgcctgcc atggtgaagc ttgcatacct gcctgcacac ggcatcatca agcttcggct
     4081 gaccggcacg ggagatgacc ctgaactggt acgcatgacg gaggaggagc aggtgcggaa
     4141 actatactcc atcatacctg aggttatcta cggtgaagat gaagtaacgc ttgaggagat
     4201 cgtaggcaag ttattgcttg acaataatct gacagtatca acagctgaga gttgtaccgg
     4261 tgggaagatt gcttcgatga tcacctcggt acccggcagc tcggcctggt acaggggatc
     4321 ggtggtggct tatgacaaca gcatcaagac cggggtgctg ggggttgacc ccggcataat
     4381 aagcaggtac ggagcagtga gtgaggagac agtgagggcc atggcggagc gaatgcggtc
     4441 aattgcagaa actgattatg cgatagcagt tacgggtata gccgggccca ccggggggac
     4501 tccggaaaaa ccagccggga ccgtctggat tgcagttgcc tctgcatctg gcgtagtagc
     4561 tgagaaacac cggtttgccg acgacaggca ggtcaatatt tcaaggtcgg ccaccaacgc
     4621 cctcaacctg cttaggaaac agataatcag caggtaatct tcgcgaaagc gactctgtcg
     4681 gaaaaaaatg cacaataaat ttggttaata gaataaaaat cacgtatttt gcgaaccgat
     4741 ttttggagtt ttc
//
LOCUS       DAOPXU010000160         4628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_23821_length_4628_cov_13.813689, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000160 DAOPXU010000000
VERSION     DAOPXU010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23821_length_4628_cov_13.813689"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PKK03_11635"
     CDS             <1..465
                     /locus_tag="PKK03_11635"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX85091.1"
                     /translation="LFGFAQAGYGNPPGNNFLSDKADIYYSLGAGLKWNIYDWGKNSN
                     EQKTLAIQQQLLEIRRGAAEESLQRMLTLKMAEIESLREAAGRDEELIGIRSRIAAAA
                     ASQLKNGTITASQYMTELNNEKQAVIAAAARRISIARAETEYLYITGYKTRE"
     gene            467..1351
                     /locus_tag="PKK03_11640"
     CDS             467..1351
                     /locus_tag="PKK03_11640"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNX85092.1"
                     /translation="MKRVVIPIVLLLAAAGCNRGESDADAWGTFEADEVIISAETPGR
                     IIRMDVTEGMLVAGGAVIAVTDTTMPVLQRGELDAVTAQAQARLAGIAAQDAVIRQQM
                     DNLAVNIERTRRMLADGAATQKQLDDLTGQMEVLRRQGEANGTQRRSVAAEQQGIAAK
                     RAVLDEQIARSTVRAPCDATVIEKYASAHELTAAGKPLVKLADMKTMKLKVWVSGAQL
                     AEVKTGTTCTVRIDQGKKEFKNYTGTVTHIAEKAEFTPKIIQTKEERVTLVYAVTIEV
                     PNDGSIKSGMPGEAIFMR"
     gene            1348..2271
                     /locus_tag="PKK03_11645"
     CDS             1348..2271
                     /locus_tag="PKK03_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX85093.1"
                     /translation="MNAVDATSITQRFGKITAVDDVSFTVREGELFGFIGPDGAGKTT
                     LFRIAATLIIPTGGRMNLFGLNVVSDFRELRNHIGYMPGRFSLYTDLTVEENLQFYAT
                     IFGTTIHENYYLIEPIYKQIEPFRKRLAGRLSGGMKQKLALSCALIHKPRILILDEPT
                     TGVDAVSRTEFWEMLEGLKREGITIIVSTPYMDEALRCDRIALMQQGKLMGIDTPAAI
                     RSSYGKRIYAVSHRDRYRLLLALRAMTEAVGPEPFGEAVHITLREGASVEDVKKSLER
                     AGLEGAEATEITPGIEDVFLGLMKRSESLKV"
     gene            2343..3086
                     /locus_tag="PKK03_11650"
     CDS             2343..3086
                     /locus_tag="PKK03_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX85094.1"
                     /translation="MEREKVIEVKDLVKRFGAFTANNRLTFDVYRGEIFGFLGANGAG
                     KTTAMRILCGLSRPTSGRVLVAGIDASRRPEEIKKRIGYMSQRFSLYEDLTVRENIEL
                     YGGIYGMSMKEIVPRREALLERLSFTSYGDMLIRALPLGLKQKLAFAVAVFHDPEIVF
                     LDEPTSGVDPVTRRQFWEMIYETASRGITVFVTTHYMDEANYCDRITIMSEGRIVALD
                     SPSALLRQYGTASVEDLFVKIARPQKNTV"
     gene            3083..4204
                     /locus_tag="PKK03_11655"
     CDS             3083..4204
                     /locus_tag="PKK03_11655"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX85095.1"
                     /translation="MKRFMAFVRKEFLHIFRDFRTLIILFGIPAAQILIFGYAVRTDL
                     KDAGIAVLDNAGDGVSKELTQKIEASEFFYVKKHLNSYEEIDEAFRQGEIKAVIIFPG
                     QIAASVAREGSATVSIIADGSEPNTASLVTGYITGIVSDYSSSLTSAVTSSLPLIVPE
                     VRMYFNPGLESHFMFIPGVITLILILICALMTSVTIVREKEYGTMEVLLVSPLKPWQI
                     IVGKVTPYFFLSLVNVIVILLMAWFVFGLPVRGSIPLLIAECMLYILLGLAIGILVSA
                     STSKMENAIFISFLALMLPTLLLSGFIFPIENMPVIYDYVSMILPPRWFVEIIRAIMI
                     KGAGFAYIWKETAILAGMTLLLMVISSRRFKTRLEEEGK"
     gene            4278..>4628
                     /locus_tag="PKK03_11660"
     CDS             4278..>4628
                     /locus_tag="PKK03_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX85096.1"
                     /translation="MKKLIFLLSKEFRQIRRNPFMIRAIIAVPVLQMLILVPAVTFEV
                     KRIDLAVIDNDRSQASRELVAKLTGSSFFVVAATPATVTEAESMLFSGDADIALVIPP
                     DFSEGLAGVTRPRLQ"
BASE COUNT         1129 a         1217 c         1323 g          959 t
ORIGIN      
        1 ctctttggtt ttgcgcaggc aggctatggc aatccccccg ggaacaactt tctttcagac
       61 aaggcagaca tatattattc gctcggggca ggactgaaat ggaacatata cgactgggga
      121 aaaaacagca atgaacaaaa gaccctggcg attcaacagc agcttctgga gatcaggaga
      181 ggtgcagcgg aagagtcgct gcagcgtatg ctcaccctga agatggcaga gattgagtcg
      241 ctgcgtgagg ctgcaggtcg cgacgaggaa ctgatcggca ttcgcagccg tattgctgcg
      301 gccgctgcct cacagctgaa gaatggaact atcactgcct cgcagtacat gacggagctg
      361 aacaacgaga agcaggcggt tattgcggcg gcggccagga ggataagcat tgccagggcc
      421 gaaacggaat atctctatat aacaggatac aaaacacggg aataatatga aaagagtggt
      481 tataccaata gttctgctcc tggctgccgc cggttgcaac cgcggtgaga gcgatgccga
      541 tgcctggggc accttcgaag ccgacgaggt gatcatatct gccgagaccc cgggcaggat
      601 catccgcatg gatgtgacgg agggcatgct ggtggcgggc ggcgccgtca tcgccgtcac
      661 cgacacgacg atgccggtgt tgcagcgggg tgagcttgat gcggtgacgg cgcaggcgca
      721 ggcgcgcctg gcgggcattg cggcgcagga cgcggtcata cgccagcaga tggacaacct
      781 cgccgtaaac atagagcgta cgcgccgcat gctcgctgac ggcgccgcca cccagaaaca
      841 gctcgatgac ctcaccgggc agatggaggt cctccgccgg cagggagaag ccaacggcac
      901 ccaacgccgt tcggtggcag cggaacagca gggcatagca gccaaacgtg ccgtgcttga
      961 tgaacagatc gcccgaagca ccgtccgcgc cccctgcgat gcaaccgtca ttgaaaaata
     1021 tgcctcggca cacgaactga cagcagcagg caaacccctg gtgaagctgg ccgacatgaa
     1081 aaccatgaag ctgaaggtgt gggtcagcgg ggcacaactg gcagaggtga agacaggaac
     1141 aacatgtacc gtgcgcattg atcagggtaa aaaggaattt aaaaactaca ccggcacagt
     1201 tacccatata gctgagaagg ctgagttcac tcccaagatc attcagacaa aagaggagcg
     1261 tgtgacactg gtctatgcgg tgacaataga ggtgccaaac gacggctcaa tcaaatcggg
     1321 aatgccggga gaggcaatat tcatgagatg aacgccgtag atgcaaccag cataacccag
     1381 aggttcggca agatcactgc cgtcgacgat gtctcattca ccgtaaggga gggcgagctc
     1441 ttcggcttca ttggtcccga cggagcgggc aagacaaccc tctttcgcat cgcggcaact
     1501 ctgatcatcc ccaccggcgg aaggatgaac cttttcgggc tcaatgtggt gagcgatttc
     1561 cgggagctgc ggaatcatat cggttacatg ccaggccgct tcagcctcta cacggatctg
     1621 accgtggagg agaacctgca gttctatgcc accatcttcg gcacaacaat acatgagaat
     1681 tactacctta tagaaccgat atacaagcag atagagccct tcaggaaacg gcttgccggt
     1741 cgtctctcgg gaggcatgaa acaaaagctt gccctctcat gcgcactgat acacaaacca
     1801 aggatcctga tcctcgacga gcccaccacc ggggtggatg ctgtctcgcg cactgaattc
     1861 tgggagatgc tggagggcct gaaaagggag gggataacca ttatcgtttc cacaccctac
     1921 atggacgagg cgctccgctg tgaccgcatc gcccttatgc agcaagggaa gctgatgggc
     1981 atagacaccc ctgcagccat acgctcatca tacggtaaga ggatttatgc cgtgagccat
     2041 cgcgacaggt acaggctcct cctggcactt cgtgcgatga ctgaggcggt cggcccggag
     2101 cctttcggcg aagcagttca tataaccctt cgggaaggtg catctgtgga agatgtgaag
     2161 aagtcgctgg aacgcgccgg cctggagggc gctgaagcaa cggagatcac ccccggcatt
     2221 gaggatgtgt ttctcggact gatgaagagg tctgaaagtc tgaaagtctg aaagtctgaa
     2281 agtctgaagg tctgaaagtc tgaaagtctg tagagcagta gagggatgaa aaaggaatat
     2341 tgatggaaag ggaaaaagtc atagaggtaa aggatctggt gaagcggttt ggggcattca
     2401 cggccaataa ccgtctcacc tttgacgtgt accggggcga gatattcggg ttcctgggag
     2461 ccaacggcgc gggcaagacc actgccatga ggatcctctg cggcctgtcg cgccccacct
     2521 cgggcagggt tctggttgcc ggtattgacg cctcgcgcag gccggaagag atcaagaaac
     2581 gaatcggtta catgtcacag cgcttctctc tgtatgagga cctcaccgta agggagaaca
     2641 tcgagctcta cggagggatt tacggtatga gcatgaaaga gattgttcct cgccgtgagg
     2701 cactgcttga aaggctcagc ttcacctcat acggtgacat gctgatcagg gcgctgcctc
     2761 tcggtttgaa acagaaactg gcattcgccg tggcggtatt tcatgacccg gaaatagtct
     2821 tcctcgacga gcctaccagc ggtgtcgacc ctgtcacgcg gcgccagttc tgggagatga
     2881 tctacgaaac cgcctcacgc ggaatcactg tctttgtcac aacccactat atggatgagg
     2941 ccaattactg tgaccgtatc accatcatga gcgagggcag gatagtagcc ctcgactccc
     3001 cctctgccct gctccgccag tacggaacgg catcggtgga agacctgttc gtgaaaatag
     3061 ccagacctca aaagaacact gtatgaaaag gttcatggca tttgtccgta aggagttcct
     3121 ccacatcttc cgcgacttcc ggaccctgat aatcctgttc gggataccgg cggcacagat
     3181 actcatcttc ggctatgccg tccgcaccga ccttaaggat gccggtatcg cagtgcttga
     3241 caatgccggt gacggggtat cgaaagagct gacccagaag attgaggcct cggaattttt
     3301 ctatgtaaag aagcacctca actcgtatga ggagattgat gaggccttca ggcagggtga
     3361 gatcaaggcc gtgatcatct tccccggaca gattgccgcc tccgttgccc gtgagggcag
     3421 cgccaccgta agcatcatcg ccgacggatc agagccgaat acagccagcc tggtgacggg
     3481 atacattacc gggatcgtga gcgactactc atcatcactt acgtctgccg tcacctcctc
     3541 actgccgctg atagtgcccg aggtaaggat gtacttcaat cccggcctcg agagccattt
     3601 tatgttcatc cctggcgtca taacgctcat actcatactg atatgcgcat tgatgacctc
     3661 agtcaccatc gtccgcgaga aggagtacgg caccatggag gtgcttctgg tatcgccgtt
     3721 gaaaccgtgg cagatcatcg ttgggaaagt gactccctac tttttcctct cgctggtgaa
     3781 cgtgatagtg attctgctga tggcatggtt tgtctttggt ctgccggttc gcggaagcat
     3841 acctctgctg atagccgagt gcatgcttta tatcctgctg ggactcgcca tcgggatcct
     3901 ggtgtcagcc tcaaccagca agatggaaaa cgcaatcttt atatccttcc tggccctgat
     3961 gctcccgaca cttcttctct cggggttcat tttccccata gagaacatgc ctgtgatata
     4021 tgattatgta agcatgatcc ttccgccaag atggtttgtt gagatcatcc gggcgataat
     4081 gatcaaggga gcgggattcg catacatatg gaaggagacg gctatccttg ccggcatgac
     4141 gctgctgctc atggtcatca gttcacgcag gttcaaaaca aggcttgagg aggaaggaaa
     4201 atgagcagga ataactgtac cgggtccgga gatggtaatg aatggaagac ctgcacgaaa
     4261 tacggggagg gcaaaagatg aaaaaactta tcttcctgtt aagtaaggag ttccgtcaga
     4321 tacgccggaa tccgtttatg atcagggcca tcattgccgt accggtatta cagatgctga
     4381 tccttgttcc ggctgtcacc tttgaagtca agcggataga cctcgccgtg attgacaatg
     4441 accgttcaca ggcttcgcgt gagcttgtcg cgaagctgac agggtcatca ttctttgttg
     4501 tggccgccac accggccacc gtcaccgagg cagagtcgat gcttttttcc ggcgatgccg
     4561 acattgcgtt ggtcataccg cctgattttt ccgagggact tgccggagtc acccgcccgc
     4621 gcctgcag
//
LOCUS       DAOPXU010000161         4584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_24170_length_4584_cov_10.939943, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000161 DAOPXU010000000
VERSION     DAOPXU010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24170_length_4584_cov_10.939943"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..833
                     /locus_tag="PKK03_11665"
     CDS             216..833
                     /locus_tag="PKK03_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX85097.1"
                     /translation="MNQMEEYDARIIAGAAELFRVYGIRAVTMDAIASHLGISKRSIY
                     ERFIDKDTLLFAVINSMIEKQRETMEGILAGSPDVITAIFTIIRMGRDHAATMNPLIS
                     ADLKKYHSKVMQKLKETCGHPDYESAGRILDMGISQGIFRDEINVEIVSRAFRGIGSL
                     TGDEELFPSERFMKRDLMRNIMVNFLRGISTPRGIELIDSMVNEI"
     gene            866..2188
                     /locus_tag="PKK03_11670"
     CDS             866..2188
                     /locus_tag="PKK03_11670"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX85098.1"
                     /translation="MKRRLIAVFMTLAAAASATLQAQDIPQALSLTVDQAVEYALDHN
                     RSVSAARYDLLASEKSVWETMAQGLPSIDASASLGYNLAIMTRIIDIGGTPQPFKFGT
                     KYDAAAGVSASAVVFNAPWLVGVETAKMASTLARQGLTQTTLDTKENVKNVYFLILTL
                     KETMKVVDANLENLNAILASTKAMYSVGMAEATDVDQMQSTVTMLENSRSSMLRSLEV
                     NQNMLRFLLGVPANTEITLTDNLDGITAQIDVDALLADEFRLEDNISYQLIETQLAMS
                     ELALKSAKASNLPSLAGSIYYNRNGMGDELMHMQYFPYSAAALQLQIPIFASGLRSAK
                     INKAKINLDKSINTKSMVSEQLLMQERQLRYNLLNANEQFKSQKANIEIAGRVLESYQ
                     NKYNQGMASSLDLTQANNNYLTAQNNYIQALMNLLQTKVEFDKLMNKL"
     gene            2218..3270
                     /locus_tag="PKK03_11675"
     CDS             2218..3270
                     /locus_tag="PKK03_11675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX85099.1"
                     /translation="MNRTIFSLILAGGILLASCAPKEKKAGASAATTADSVAAIPVKI
                     MPVSKTMIARTIDYTSTIQAYEEVNLAPSTPGRVDKIYVEVGDRVQKGQKLFLMDRTQ
                     YYANKIQLANLEKDLARLDTLLKVGSVKEQLYDQTKAQYEVMKTNVDFMEENTMLEAP
                     FSGIITGKYLEDGELYSGAPGMSGKAAVVTLMQINPVKIIISISEQYFPLIKNGMKVR
                     IVADVYPDKSFEGTIFRIHPTINAMSRSFNAEVRISNGSELLRPGMFARSFIDMGQEE
                     AIVVPANAVMMQEGTNERYIFVTENGIAVRKPVTLGQRFDDRFEIAGGALKEGDNLVT
                     EGQARLNNGQKVDITR"
     gene            3297..>4584
                     /locus_tag="PKK03_11680"
     CDS             3297..>4584
                     /locus_tag="PKK03_11680"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX85100.1"
                     /translation="MSIYASAVKKPVTTILIFVITMVMGLYSLTQLPIDLYPEIELPF
                     LSVYTTYAGASAADIETNITRPIEDALNSVSGLKEMTSTSSDNVSVIFMEFEYETNLD
                     EASNDVRSSLAFIESFLPEDAEDPTIFKFNSSMMPIIFYAITAEESYKGLEKILDEKL
                     VNPLNRIDGVGNVGLSGVPGREIYVEVDPRRMEAYNLTIEQVGNVLRAENLNMPAGYL
                     EMGKTDYPIRIQGEFAESDLIANVIVGNFNGNNVYLRDIATVNDTIRETKLVSKIDGK
                     QGMTLFVQKMSGANSVEICREVDAEITKLQKDLPPDIRIEKIMDTSEFIQGSINNLTE
                     TLMYAALFVVLVVLFFLGRWRATLIIILTIPISLLVAFVYIFLTGSSINIISLSSLSI
                     AIGMVVDDAIVVLENITKHIERGSRPREAAIYATNEV"
BASE COUNT         1305 a         1166 c         1140 g          973 t
ORIGIN      
        1 ccggacctcc ggaccttcag accctcagac cttcagaccc tcagaccttc agaccttcag
       61 accttcagac cttcagacct tcagaccttc agaccttctt tatccattca ccggccattt
      121 accgcaactc ggcgggattt tacaaatatt tgacagaaaa aactaggaaa actcaaaaag
      181 ttttctgaat attgcgccct gttcccgaaa acattatgaa ccagatggaa gaatatgatg
      241 ccagaattat agccggtgct gcagagctgt tccgggtgta cggcattagg gcagttacca
      301 tggatgccat cgcttcccac ctggggatct cgaaaagaag catctatgag aggttcattg
      361 acaaggatac cctgcttttt gcagtgatca attcaatgat cgaaaaacaa agagagacaa
      421 tggaaggaat tctggcaggc tcacctgacg taatcactgc catcttcacg attatccgga
      481 tgggaaggga tcatgctgca accatgaatc cgctgatcag cgccgacctt aagaaatacc
      541 acagcaaggt aatgcagaaa ctgaaggaaa catgcggaca tcccgactat gaatcagccg
      601 gcaggatact ggatatgggg atcagccagg gtatcttcag ggatgagatt aatgtggaga
      661 ttgtcagcag ggccttcagg ggaataggtt ccctgaccgg tgacgaggag ctctttccca
      721 gcgagaggtt catgaagagg gacctcatgc gtaacataat ggtgaacttc ctgaggggca
      781 tttcaactcc ccggggaata gaactgattg attcaatggt aaatgaaata tgattttaac
      841 acaggaacag ataataggta accatatgaa aagaagatta attgctgttt ttatgactct
      901 tgccgctgca gcctctgcca cactgcaggc gcaggatatc ccgcaggctc tctcgctgac
      961 ggtggaccag gcggtggagt acgcccttga ccacaacagg agcgtgtcag ctgcacggta
     1021 tgacctgctt gcatcggaga agagtgtctg ggaaaccatg gctcaggggc ttccttcaat
     1081 tgacgccagc gcctcactgg gttacaacct tgcaatcatg acaaggataa ttgacatagg
     1141 cggcacacca cagcctttca agttcggtac caagtatgac gcagctgcag gggtgtcggc
     1201 atcggcagtt gtattcaacg caccatggct ggtgggagtc gagaccgcaa agatggcttc
     1261 cacacttgca cgccagggac tgacacagac aacccttgac accaaggaga atgtcaagaa
     1321 cgtatacttc ctgatcctca cactgaagga aaccatgaaa gttgtggatg ccaaccttga
     1381 aaacctgaat gcaatacttg catcaaccaa agcaatgtac agtgtcggca tggccgaagc
     1441 cacagacgtg gatcagatgc agtctaccgt taccatgctt gaaaactcaa ggtcatcaat
     1501 gctccgctca ctggaggtta atcaaaacat gctgaggttc cttctcggag ttccggctaa
     1561 cactgagata acccttaccg acaaccttga cggaataacg gctcagattg atgttgacgc
     1621 ccttcttgct gatgagttca ggctggaaga caatatcagc taccagctca tcgagaccca
     1681 gctggctatg tcggaactgg cgcttaaaag cgcgaaggcc agtaaccttc ccagtcttgc
     1741 gggttcgatc tactacaaca gaaacggcat gggtgacgag ctgatgcaca tgcagtactt
     1801 cccctattcg gcagctgcac tgcagctcca gattccgata ttcgcatccg gactgagaag
     1861 tgcgaagatc aacaaggcga agatcaatct tgacaagagc atcaatacaa aatcaatggt
     1921 ttccgagcag ctgctgatgc aggagaggca gttacggtac aacctgctta acgcgaatga
     1981 acagttcaag agccagaagg caaacataga gattgcaggc cgggtcctgg aaagctatca
     2041 gaacaagtac aaccagggga tggcttccag ccttgacctg acacaggcca acaacaacta
     2101 cctgactgcc cagaacaact atatccaggc ccttatgaac cttctgcaga ccaaggtgga
     2161 atttgacaag cttatgaaca aactgtaaca gaaaaacaac aataaataaa gaatacaatg
     2221 aaccgcacaa ttttttcact gatccttgcc ggaggcattc tgcttgcatc atgtgcccca
     2281 aaggaaaaaa aggccggagc ctctgcagca acaactgcag attcagttgc agccatcccg
     2341 gttaagatca tgccggtcag caagaccatg attgcaagaa caatagacta tacatccact
     2401 attcaggcat acgaggaggt taatctcgcc ccctcaaccc cgggcagggt tgacaaaata
     2461 tacgtcgaag tgggtgacag ggtgcagaag ggacagaagc tcttcctgat ggaccgcaca
     2521 cagtattacg ctaacaagat tcagctggca aacctggaga aggacctggc gcggcttgac
     2581 accctgctca aggtcggaag cgtgaaggag cagctctatg accagacgaa agcgcaatac
     2641 gaggttatga agacaaacgt agatttcatg gaggagaaca ccatgctcga ggctccgttt
     2701 tccggaatca taaccggcaa ataccttgag gatggcgaac tctattcggg agcacccggc
     2761 atgtcaggca aggctgccgt tgtcaccctg atgcagatca atcccgtgaa aatcatcatc
     2821 agcatctcgg aacagtattt cccgcttata aagaacggca tgaaagtgcg tatcgtggct
     2881 gacgtatacc ctgacaaaag cttcgaagga accatcttca ggatacatcc taccattaat
     2941 gccatgtcaa ggtcattcaa tgccgaggta cgtatcagca acggcagtga gttgcttcgt
     3001 cccggcatgt tcgccagatc atttatagac atgggacagg aagaagccat cgtggttccg
     3061 gccaacgcag taatgatgca ggagggaacc aatgaaagat acatcttcgt cacggagaac
     3121 ggtattgcag taaggaaacc ggtcaccctc gggcaaaggt ttgatgaccg cttcgagatc
     3181 gccggcggcg cccttaagga gggtgacaac cttgtcaccg aaggccaggc acgcctcaac
     3241 aacggccaga aggtcgatat caccaggtaa tgacaaacaa gagacaatag cgcattatga
     3301 gtatatatgc cagtgcagta aagaagcccg tcaccacgat actgatcttc gtgatcacga
     3361 tggtcatggg gctttattcc ctgacgcagc tgccaatcga cctctaccct gagatcgagc
     3421 tccccttcct gtcggtttac acaacctacg cgggagccag cgcggcggac attgaaacga
     3481 acataacccg tccgatagag gacgccctca actccgtctc cggtcttaag gagatgacat
     3541 ccacatcgag tgacaacgtt tcggtcatct tcatggaatt cgaatatgag acgaacctcg
     3601 atgaagcgtc aaatgacgtc aggagttccc tagccttcat tgaaagcttc cttccggaag
     3661 atgccgagga tcccacaatc ttcaagttca actccagcat gatgcccatc attttttacg
     3721 ccatcaccgc tgaggagagc tacaagggac tggaaaagat actcgatgag aagctggtta
     3781 atccccttaa ccgtatcgat ggcgtcggta acgtcggact ttccggtgta ccgggaagag
     3841 agatatacgt tgaggttgac ccgcgacgga tggaggccta caacctgacc attgaacagg
     3901 ttggaaacgt gcttcgagcc gagaacctga acatgccggc aggttacctg gagatgggca
     3961 aaaccgacta ccccattagg attcagggag aatttgccga gagtgacctg attgcaaacg
     4021 taatcgtcgg caacttcaac ggaaacaacg tttacctgcg tgacatcgcc acggtaaacg
     4081 atacaatcag ggaaacaaaa ctggtttcaa agatcgacgg caagcaggga atgaccctct
     4141 ttgtgcagaa gatgtcaggg gccaactcgg ttgagatctg ccgcgaggtg gatgccgaaa
     4201 tcacaaagtt gcagaaagac ctcccgcctg atatccggat agaaaagata atggatacct
     4261 cggaattcat ccagggctca atcaacaacc tgactgagac gctgatgtac gctgccctgt
     4321 tcgtggtgct ggtggtgctc ttcttcctcg gcagatggcg tgcaacgctg ataatcatcc
     4381 ttaccatccc catctctctg ctggtggcct ttgtctacat attcctgacg ggaagctcca
     4441 tcaacatcat ttcgctctcg tcactgtcaa ttgccatagg tatggtggtt gacgacgcca
     4501 tcgtggtgct ggagaacatc accaagcaca ttgagagggg aagccgtccg cgcgaggcag
     4561 ccatctacgc caccaacgaa gtgt
//
LOCUS       DAOPXU010000162         4572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_24257_length_4572_cov_8.445207, whole genome shotgun sequence.
ACCESSION   DAOPXU010000162 DAOPXU010000000
VERSION     DAOPXU010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24257_length_4572_cov_8.445207"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1863)
                     /locus_tag="PKK03_11685"
     CDS             complement(85..1863)
                     /locus_tag="PKK03_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85101.1"
                     /translation="MKAFLLAVALAAITVEVASQPLPGFRPSGQFDEQQTVIENSVAG
                     TRVLINAPMTGFGFDDRVLLVLYALPNGNTIEQTFGKKLKEGDDWHYDIQHIGAQTRF
                     LRRVITDRTVVVAYLENERKSWPAWSASNSGPEEQVKRTVEEIAALFAPWRTELVLNG
                     HSGGGRFIFDYLDAHQRIPDNVVRIAFLDSNYGWEDDRYGPKMVSWLQSGRNRYLCTL
                     AYNDSVVIYNGKPLVSPTGGTWYRSGVMRDYLARSFSLKRRERDSLVWYSSRDRRIEF
                     IFKPNPEGKVFHTHQVEYNGFIHSMLSGTRQEQHGYRYFGVRAYQDLISDTVIVPVRR
                     LNIPPRDPSSVTGSEFMKRINDLSREEREEEIYRAAACGNIPDFLRETVTLRAVFSDM
                     NGVQHMLEYEVMPDYLAIGSDEDYCRIPMTPGTAQRLADLFGASLLTAKLSDHIWSVA
                     EVKPEPFFYRPVGNENELVKKFFEHNEQIERQLADAGWKHGQLVAGIKKDVIISSRIS
                     ERPDRVVIYGWHRSDGSPIQPVYSGHIWWYVDYSHGIRLMNAQVLVDGRPVKVSDLLK
                     DPVLFRIITNEENPVEMSFYPKNVTL"
     gene            complement(1860..3212)
                     /locus_tag="PKK03_11690"
     CDS             complement(1860..3212)
                     /locus_tag="PKK03_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HNX85102.1"
                     /translation="MKWVFAILLFLAAGILVNAQLPAFPGAEGFGRYTTGGRGGAVYT
                     VTNLADSVVKPPEGSLRWALNKPSPKTIVFAVSGTIDLKRRLNVSKGDVTIAGQTAPG
                     DGICIKGETVSIEADNVIIRHIRFRCGNETPGEEPKDAISCIRHRNIIIDHCSMSWSV
                     DEAASFYDNENFTLQWCIISESLYDAGHPKGEHGYGGIWGGNGASFHHNLLASHTSRL
                     PRFCGARYHPDTRETELVDFRNNVIFNWGFNSSYGGEMGQQNMVNNYYKPGPATKKDV
                     ISRIVEPWDTVGRWHVSGNRIEGNSKVSSDNWSGGVQGDNAGNPVIREDEPFVVADVR
                     TTSARRAYRSVLRNAGATLPHRDPVDARIIDETRAGFCAFGDSYGAGTGIIDTQNSAG
                     RWPLLKTYDVLADNDGDGMADSWELKKGLNPADPEDRNNLSGSGYTMLEEYINGLCRR
                     "
     gene            complement(3209..>4572)
                     /locus_tag="PKK03_11695"
     CDS             complement(3209..>4572)
                     /locus_tag="PKK03_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HNX85103.1"
                     /translation="LKSNLRMHAEEGALVLFSPDKSLYPLIETSFEGYNTVRCISPVY
                     GKDLENIAFTGKGVWDGSGHFWRHVKKEKVPPPMWNDLVRSGGTVSTDGKTWYPSEAY
                     GRAIEQSEMNVPLPMEGGIEAYEWMRDFLRPVMVSLVSCKKILIDGPVFQNSPAWCIH
                     PLMCQDLTVRNITVRNPWYSQNGDGIDIESCRNTLLYNCNFDVGDDAICIKSGKNEDG
                     RKRGMPTENLVIRNCIVYHGHGGVTIGSEMSGGVRNVSVAGCTFMGTDVGIRFKSNRG
                     RGGVVENIWFNDILMTDIPSQAISFNLYYGGLSVSEMLAEGKNIETTVGDIPPVTEET
                     PQFRNISMKNITCRGADQAIYLQGLPELNLENITMENIDMTAVEGMACIDARGIKIRN
                     MRLVTEKKPLIFFLNSSDADVTGLQVAEDADGLVEVRGSSSARITVGTVTPDGTARAR
                     MFR"
BASE COUNT         1033 a         1321 c         1062 g         1156 t
ORIGIN      
        1 ccgataagaa gagaaacgag tatttccatc tcaatcagtt tttaggatta aacttcaaag
       61 aatgggaaat gtactaaata aaatttacaa tgtcacattt ttaggataaa aagacatctc
      121 aacgggattt tcttcattgg tgataatcct gaaaagcacc ggatctttca aaagatcaga
      181 aactttaacc ggacggccat caaccagcac ctgtgcgttc atcagcctga tgccgtgact
      241 gtagtcgaca taccaccaga tatggccgct gtatacgggc tggatggggc tgccgtcaga
      301 gcggtgccag ccgtatatca ccaccctgtc aggccgctct gaaatacgtg acgaaattat
      361 aacatccttc ttgatccctg caacaagctg accgtgcttc cagccggcat cagccagctg
      421 cctctcaatc tgctcattgt gctcaaaaaa cttcttcacc agctcattct cattgccgac
      481 aggcctgtaa aagaacggct caggcttcac ctcagccaca gaccatatat gatcactcag
      541 ttttgctgtc agcaaagaag ccccaaacag gtcagccagc cgctgtgctg ttccaggagt
      601 catgggaatc cggcagtaat cctcatcact tcctatggcc aggtaatcag gcattacctc
      661 atattcgagc atatgctgaa cgccgttcat atcactgaac actgccctga gggtgacggt
      721 ctcacgcagg aaatccggaa tatttccgca ggcagctgcc cggtaaattt cttcctcccg
      781 ttcctcgcgt gaaagatcat tgattcgctt catgaactcc gaacccgtca cagatgacgg
      841 atcacggggc gggatattaa gcctcctgac ggggacaatg acggtatctg atatcagatc
      901 ctggtaagcc cgcaccccga aatacctgta tccatgctgt tcctgcctgg ttccactcag
      961 catactgtga ataaatccgt tatactccac ctggtgggta tggaaaacct ttccttccgg
     1021 gtttggtttg aagatgaatt cgatccggcg gtcacgcgat gaataccata caagcgaatc
     1081 acgctcccgc cttttcaggc tgaatgaccg ggcaagataa tcacgcatga caccgctccg
     1141 gtaccatgta cccccggttg gagaaaccag cggtttgccg ttgtagataa ccacgctgtc
     1201 attgtaggcc agtgtgcaga gatacctgtt tctgcccgac tgcaaccagc tgaccatctt
     1261 cggtccgtac cggtcatctt cccatccgta attgctgtcg aggaaggcta tccgtaccac
     1321 attgtccggt attcgttggt gagcatccag atagtcgaat atgaaccgcc ctccgccgct
     1381 gtggccgttc agaaccagct cagtcctcca cggggcaaaa agggcagcta tctcctcaac
     1441 ggttcttttg acctgttcct ccgggccgga attgctggcg ctccatgccg gccagctctt
     1501 tcgctcattc tcaagatagg ccaccaccac ggttctgtca gttattaccc ttctcaggaa
     1561 cctcgtctgg gcgcctatgt gctggatgtc atagtgccag tcatcgccct ctttcagctt
     1621 cttcccgaaa gtctgttcaa tggtgttacc gtttggaagt gcatagagta caagcagcac
     1681 ccggtcatcg aagccaaatc cagtcatggg ggcgttgatg agcaccctgg tgccagcaac
     1741 tgaattttca ataactgtct gttgttcatc aaattgccct gacggtctga atccgggcag
     1801 gggctgcgag gcaacctcaa cggtgatggc tgccagggcg acagccagaa ggaaagcttt
     1861 catctcctgc agagaccgtt tatgtactcc tcgagcatag tatagcctga ccctgacagg
     1921 ttattccggt cttcaggatc ggccggattc agtccctttt taagttccca tgagtcagcc
     1981 atgccatcac cgtcattgtc ggccagtaca tcatatgttt tcagcaacgg ccatctgccg
     2041 gcactgtttt gtgtgtctat tatcccggtt ccggcaccgt acgaatcgcc aaaggcgcag
     2101 aagccggccc gggtttcatc aattatccgg gcatcgaccg gatccctgtg aggcagggtg
     2161 gctccggcat ttctcaagac cgacctgtaa gcacgcctgg cggaagtggt tctcacatct
     2221 gcaacaacaa acggttcatc ttccctgatg acagggttgc cggcattatc accctgaaca
     2281 ccaccgctcc agttgtcaga tgagacttta ctgttgcctt cgatgcggtt tccgctgaca
     2341 tgccatctgc caacagtgtc ccatggttcc acaatacggc tgatcacgtc tttttttgtt
     2401 gccggtccgg gcttgtagta gttattgacc atattctgct gccccatctc gccaccgtac
     2461 gagctgttaa aaccccagtt gaagatgacg ttgttccgga aatcgaccag ctccgtctca
     2521 cgtgtatccg ggtgatatct tgctccacag aaacggggca ggcggctggt gtggcttgcc
     2581 aggaggttat gatggaatga ggctccattt ccgccccata tcccgccata cccatgctcg
     2641 cccttcggat ggccggcatc ataaaggctc tcggagatga tgcaccattg aagagtaaag
     2701 ttctcgttgt catagaacga agcggcctcg tcaacagacc agctcatgga gcagtgatct
     2761 atgatgatgt tccggtgcct gatgcatgag atggcatcct tgggttcttc acccggggtc
     2821 tcattgccac aacggaacct gatatgcctg attataacgt tatcagcctc tattgaaact
     2881 gtctccccct taatgcatat tccgtcaccg ggcgccgtct gcccagcaat ggtgacatcg
     2941 cccttcgaga cgttcagtct ccgcttcagg tcaatggtac ctgaaactgc gaacacaatt
     3001 gtcttcggcg acggcttgtt aagcgcccat ctcaacgagc cttcaggagg cttaaccacc
     3061 gaatcggcaa ggtttgtgac ggtatagacc gctccgcccc ttccgccggt ggtataccgg
     3121 ccgaatccct cagcgccggg aaatgccggc aactgggcat tcaccaggat gccggctgcc
     3181 agaaaaagca atattgcaaa aacccatttc atctaaacat cctcgctctg gctgtcccgt
     3241 caggtgtaac tgttccaacc gttatacggg ctgaactgct gccgcgaacc tcaacaagac
     3301 cgtctgcatc ttcggcaacc tggagtcccg tgacatcagc atccgagctg ttgagaaaga
     3361 agatcagcgg tttcttctcg gtcaccagcc gcatgttcct gatctttatc cccctggcat
     3421 caatgcaagc catcccttcc actgcagtca tgtcgatgtt ttccatggtt atattctcaa
     3481 ggttcagttc aggcaatccc tgaagatata tcgcctggtc agcacccctg caagttatgt
     3541 ttttcatgga aatgttcctg aactgcgggg tttcttcagt gaccggaggt atatcaccaa
     3601 ccgtcgtctc tatattcttc ccttctgcaa gcatctccga gaccgagagg cccccgtaat
     3661 acaggttgaa cgaaattgcc tgcgagggga tgtctgtcat caggatgtca ttgaaccaga
     3721 tattttctac cacccctccc ctgccgcggt tgctcttgaa acgtatgccc acgtcagtgc
     3781 ccatgaaggt gcatccggca accgatacat tccttacgcc gcctgacatc tcgctcccta
     3841 ttgtcactcc tccgtgaccg tgatagacaa tgcagttcct tatcaccagg ttttcagtgg
     3901 gcatgccccg ctttcttccg tcctcattct tgcccgactt gatgcatatg gcatcatctc
     3961 ccacatcgaa gttacagttg tacagcagag tgttgcggca tgactctata tctatcccgt
     4021 ctccgttctg ggaataccag ggatttctga cagtgatatt ccttacagta agatcctggc
     4081 acataagcgg atgaatgcac catgcggggg agttctgaaa tacaggcccg tcaatcaaaa
     4141 tctttttaca gctgaccagg cttaccatga ctggacgaag gaaatccctc atccattcat
     4201 atgcctcaat gcctccctcc atcggcaacg gtacgttcat ctcgctctgc tcgatggccc
     4261 tgccgtatgc ctcagaaggg taccatgtct tcccatcggt gcttaccgta cctcctgatc
     4321 tgacaaggtc attccacatc gggggaggca ccttttcctt cttcacatgc ctccagaagt
     4381 gaccgctgcc atcccagaca cctttcccgg tgaaggcaat gttttcaagg tccttgccat
     4441 agaccggcga gatacacctg actgtattgt agccctcgaa gcttgtttct ataagcggat
     4501 acaggctctt gtcggggctg aacagcacca gggcgccctc ctcggcgtgc atcctcaggt
     4561 tgcttttcag ta
//
LOCUS       DAOPXU010000163         4567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_24306_length_4567_cov_14.734707, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000163 DAOPXU010000000
VERSION     DAOPXU010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24306_length_4567_cov_14.734707"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..3343)
                     /gene="carB"
                     /locus_tag="PKK03_11700"
     CDS             complement(125..3343)
                     /gene="carB"
                     /locus_tag="PKK03_11700"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HNX85104.1"
                     /translation="MKDDIKKVLLLGSGALKIGEAGEFDYSGSQALKALREEGIYTVL
                     INPNIATVQTSEKIADKIYFLPVTPKFVERVIEREKPDGILLSFGGQTALNCGTALFR
                     TGVFEKFAVRVLGTPVEAIMNTEDRELFVKKLDEISVKTPRSVAVTSVEDALSAAGQL
                     GFPIIIRAAYTLGGQGSGFCSNTDELRTMAARAFSYSDQILVEESLKGWKEVEYEVVR
                     DRFDNCITVCNMENFDPLGIHTGESIVVAPSQTLTNSEYHKLRELSIKIIRHIGIIGE
                     CNVQYALDPGSEDYRVIEVNARLSRSSALASKATGYPLAFVAAKLALGYGLHQLKNSV
                     TRSTTACFEPALDYIVCKIPRWDLNKFQGVAHEIGSSMKSVGEIMAIGRTFEEAIQKG
                     LRMIGLGMHGFVSNRNLSFNDIEKELSQPTDMRIFSIAEALEKGFDVDRIWELTRIDR
                     WFLYKLQNISHIKKELEKYDSMESLPDRELLHAKINGFSDFQIARHVLKSENRAINEA
                     MLSVRRHRRSRGIVPYVKQIDTLAAEYPAMTNYLYLTYSGMEHDIWYENDKRSVIVLG
                     SGAYRIGSSVEFDWCSVNAINTIRKEGLRGVMINYNPETVSTDYDMCDRLYFDELSFE
                     RVMDIIDLETPRGVILAMGGQIPNNLAMRLHRQGVPILGTPPESIDRAEDRHKFSSMC
                     DALDIDQPRWKELRSFEEINKFIEDIGFPVLIRPSYVLSGAAMNVVSNSTELVHFLEL
                     AASVSKEHPVVVSEFIEYAKEIEIDAVASGGDIVAYAISEHVEYAGVHSGDATIVFPP
                     QKIYFETVRRIKRITREIARELNISGPFNIQFLARENDIKVIECNLRASRSMPFVSKV
                     LKTNLIELATRVMLGVQWEKPHKSVFDLDYVGVKASQFSFSRLAKADPVLGVDMASTG
                     EVGCIGDSFHEALLKAMYSVGYRMPVKAVLISSGPAKSKADLLEAVRLLEKRGYKIFA
                     TRGTQQFLSNAGIAAKVAHWPDEAASPNTVELIKLRVVDLVINIPKDLSATELSNDYQ
                     IRRSAVDYNIPLITNARLASAFINAFCSVPESEMEIKAWDEY"
     gene            3443..3904
                     /locus_tag="PKK03_11705"
     CDS             3443..3904
                     /locus_tag="PKK03_11705"
                     /inference="COORDINATES: protein motif:HMM:NF013483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HNX85105.1"
                     /translation="MNRTSRLALIEKLVREHVIGSQDELLRMVEGTGMKCTQATLSRD
                     LRQLGISRGFDGSGGYRYILPTGREEAAETVPGSTAAAITGMTWARGLLLISTPPGFA
                     SAVAIRIDRAGRYEIAGTVAGDDTILLIPRDNISNEAVEECLRTIFQSEIK"
     gene            3916..>4567
                     /gene="argG"
                     /locus_tag="PKK03_11710"
     CDS             3916..>4567
                     /gene="argG"
                     /locus_tag="PKK03_11710"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNX85106.1"
                     /translation="MKKVMLAYSGGLDTSVILKWLINKGYEVIAYVADVGQNDDFEAV
                     REKALAIGASSVIIEDLKREFVTDYIFQAVKANAVYEDRYLLGTALARPLIAKKQIEA
                     AIEYEADYVAHGATGKGNDQVRFELAYYALKPDIKVISPWKDAEFLSQFQGRSDMIRY
                     AAGHRIPVKASISKPYSEDDNLMHISHEAGILEDPLYFAGKDILQKMVMPMDAPDME"
BASE COUNT         1075 a         1259 c         1150 g         1083 t
ORIGIN      
        1 tcatacttta attatctaat ctgatctaca tttctttata cccaacggcc aaatcgcaaa
       61 atcgccaatc gccaatcgcc aatcgcaaaa tcgcaaatcg caaatcgcaa atcgcaaatc
      121 ctcatcagta ctcatcccat gccttgatct ccatctcgct ctcgggaacg ctgcagaagg
      181 cattgatgaa ggccgaggcc agacgcgcgt tggtgatcag cgggatattg taatcaaccg
      241 cgctgcgccg tatctggtaa tcattgctca gctccgtagc cgacaggtcc ttcggtatgt
      301 tgatcaccag gtcaaccaca cgcagtttta tcagctccac tgtatttggc gatgccgcct
      361 cgtccggcca gtgcgccacc ttcgcggcaa tgccggcatt ggaaaggaac tgctgggtgc
      421 cccgcgtggc aaagatctta taaccccgct tttccagtaa ccttacagcc tccagcagat
      481 cggccttcga tttggcaggc cctgatgaga tcagcactgc ctttaccggc atcctgtaac
      541 ctaccgaata catcgccttt agcagcgcct catggaagct gtcgccaatg caacccacct
      601 caccggtgga agccatgtcc actcccagca ccgggtcagc cttggccagt cgcgagaaac
      661 tgaactgcga tgccttaacc cccacataat caaggtcaaa aaccgacttg tgcggtttct
      721 cccactgaac ccccagcatt acccttgtgg ctaactctat caaattggtc ttcagtacct
      781 ttgagacaaa tggcatgctg cggctggctc gcaggttgca ttcaatgact ttgatgtcat
      841 tctcacgtgc caggaactga atgttaaatg gccccgaaat attaagctcc cgggctattt
      901 cgcgggtgat acgcttgatg cgccttaccg tctcgaaata aattttctgc ggagggaaaa
      961 caatggtggc atcgcccgaa tgtaccccgg cgtactcaac atgctcactg atggcatatg
     1021 ccacaatatc accgcccgat gccacggcat caatctctat ctccttcgca tactctatga
     1081 attcagacac caccaccggg tgctccttgg aaacgctggc cgcaagttcc aggaaatgga
     1141 ccagctcggt gctgttcgaa accacgttca tcgcggcacc cgagagcaca tatgaaggcc
     1201 gtatgagcac cgggaatcct atatcttcaa tgaacttgtt gatttcttcg aaactccgga
     1261 gctccttcca gcgtggctgg tcaatatcaa gtgcgtcaca catcgaggag aacttatggc
     1321 ggtcctccgc acggtcaatc gactccggcg gcgtgcccag tatcggaacg ccctgccggt
     1381 gcagcctcat cgccaggtta ttgggtattt gtccgcccat ggcgagaata actccgcgtg
     1441 gcgtctccag gtcaatgata tccataaccc tttcaaacga caattcgtcg aaatagagcc
     1501 tgtcgcacat gtcatagtcg gtgctgacag tctccgggtt gtagttgatc atcaccccgc
     1561 gcagaccctc tttcctgatg gtgttgatgg cgttgaccga gcaccagtca aactcaacgc
     1621 tcgagccaat cctgtaagcg cctgacccaa gcactatcac ggagcgcttg tcattttcat
     1681 accatatgtc atgctccatc ccgctgtaag tgagatagag ataattggtc atggcgggat
     1741 actcagccgc cagtgtatca atctgtttga catagggtac aattccacgg cttctcctgt
     1801 gtcgcctgac gctgagcatg gcttcattga tggccctgtt ttctgacttc agcacatgcc
     1861 gtgcgatctg gaaatcactg aacccgttta tcttggcgtg aagcagctcc ctgtcgggca
     1921 gactctccat ggagtcatat ttctcaagct ctttcttgat atggctgata ttctgaagct
     1981 tgtaaaggaa ccagcggtca attctggtga gttcccagat gcggtcaaca tcaaaaccct
     2041 tctccagagc ctcggcaata gagaagatgc gcatgtctgt gggttgtgaa agctcctttt
     2101 cgatgtcatt aaagctcagg tttcggttgc tgacgaaacc atgcatcccc agtccgatca
     2161 tgcggaggcc cttttgtatt gcctcctcga aggtgcgccc gatggccatg atctcaccaa
     2221 cgctcttcat gctgcttcct atctcatggg ccaccccctg gaacttgtta aggtcccacc
     2281 gcgggatctt gcagacgata taatcgagcg ccggctcgaa gcatgcagtt gtggatctgg
     2341 tgactgaatt cttcagctgg tgcaggccgt agcccagggc cagtttggca gccacaaatg
     2401 ccagcggata gccagttgcc tttgacgcca gcgcgctgga gcgcgaaagc cgggcgttga
     2461 cctcgatgac acggtagtcc tccgagccgg gatcgagggc gtattgcaca ttgcactcgc
     2521 cgataatacc gatatggcgg ataatcttta tcgacagctc cctgagcttg tggtactcac
     2581 tgttggtaag cgtctgcgaa ggagccacca cgatactctc accggtatgt atcccgaggg
     2641 gatcgaagtt ctccatgttg cacaccgtga tgcagttgtc gaaccggtcg cgcaccacct
     2701 catattccac ctctttccag cctttgagcg attcctccac cagaatctgg tcagagtagg
     2761 agaaggcgcg ggcggccatc gtcctgagct catcggtatt tgagcagaat ccgcttccct
     2821 gcccgccgag ggtatatgca gcgcggatga tgatagggaa tcccagttgt ccggccgcgg
     2881 agagcgcatc ctccacgctg gtgacggcca cgctgcgggg agtctttaca cttatctcgt
     2941 caagtttctt tacgaagagt tcccggtcct cggtgttcat tatagcttcc accggtgttc
     3001 ccagcactct cactgcgaac ttctcaaata ccccggtgcg gaagagagcc gtgccgcagt
     3061 tgagtgccgt ctgccctccg aatgagagga gtatcccgtc aggcttctcg cgctcaatga
     3121 cccgctccac aaacttcggg gtaaccggaa gaaagtagat cttgtcggcg atcttctccg
     3181 aggtctgaac agttgcaata ttcggattga taagtacggt gtagattccc tcttcgcgca
     3241 gggctttcag cgcttgcgat ccggagtagt caaattcacc tgcttcaccg attttaaggg
     3301 ctcccgaacc gagcagcagc acctttttaa tatcatcctt catcgttcct gtttggattg
     3361 taaaaataca atttttgcga cctgaatgct tgcaataata aaaattgtat aaatttgcat
     3421 ctgatatgta tataaataca gtatgaacag aacgtcacga ctggcgctga tagagaagct
     3481 ggtcagggag catgtgatcg gctcacagga tgaattgctc aggatggtgg agggcacagg
     3541 gatgaagtgt acgcaggcga cactctcccg cgatttaagg cagctgggta tcagcagggg
     3601 atttgacggc agcgggggtt accgctatat tctacccaca ggcagggagg aggctgctga
     3661 gacggttccg ggcagcactg cggctgcaat taccggcatg acatgggcaa ggggacttct
     3721 gcttatcagc accccgccgg gatttgcatc agcagtagcc atcaggatag accgagccgg
     3781 ccgttacgaa atcgcaggaa cagttgccgg tgacgacaca atcctgctta tcccgcgtga
     3841 caacatcagc aacgaggcag ttgaagagtg cctccgcaca attttccagt cagaaattaa
     3901 ataacagaga acagaatgaa aaaagtaatg cttgcatatt ccgggggact cgatacctcc
     3961 gtgatactca aatggctcat caacaaagga tacgaagtaa ttgcctacgt ggccgacgta
     4021 ggacagaatg atgatttcga ggcagttcgc gaaaaggccc ttgccatagg ggcctcctcc
     4081 gtgattattg aggacctgaa aagggaattc gtcactgatt atattttcca ggcagtgaag
     4141 gcaaacgctg tttacgaaga ccgctatctc ctcgggactg cgctggcacg tccgctgatc
     4201 gcgaagaaac agattgaggc tgccatagaa tacgaggctg attatgtcgc ccacggcgca
     4261 accggtaagg ggaatgacca ggtacggttc gagcttgcct attatgccct taaacctgat
     4321 atcaaggtta tatcaccctg gaaggatgct gaattccttt cgcagttcca gggcaggtca
     4381 gacatgatca ggtatgctgc cggtcaccgc atcccggtca aggcatccat ctccaagccc
     4441 tacagtgagg acgacaacct gatgcatatc agccatgagg caggtatcct tgaggatccg
     4501 ctctacttcg ccgggaagga tatccttcag aagatggtga tgccaatgga tgcgcctgat
     4561 atggaga
//
LOCUS       DAOPXU010000164         4535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_24557_length_4535_cov_11.242857, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000164 DAOPXU010000000
VERSION     DAOPXU010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24557_length_4535_cov_11.242857"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1902
                     /locus_tag="PKK03_11715"
     CDS             100..1902
                     /locus_tag="PKK03_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HNX85107.1"
                     /translation="MKRMIIIAIFLTVALALAAQEEKKLTILHTNDFHSHLQGYAPES
                     AYTPGVADNDPTIGGMARIAGIIEAVRNENPGSALVVDAGDCLMGTLFQALEPETGFQ
                     HALMKKAGYDVVAMGNHDFDFGPEAFAGILRKASQRGEIPLILTGNAITDPDDPADDA
                     FEALISDGLIKPYTVKEINGIRIGLFSLIGKDADESAPYAPPVTFGKIIPAAKKLVRN
                     LKKEGCDVIICLSHSGIRKDDKGEWTGEDVKLASKVKGIDLIISGHTHTLLKEPLVVN
                     AVPIVAVGDNGRFVGRIDARVINGSLKLEKYEAIPVDDRTTALEEIQSEIVLQQQKIN
                     EAILNPIGMTYTMPVAIAPYTVSYDEYGKAADSNLGVLVADAIYNYVNQEGPGTDIAI
                     VAAGVLRDPIQPGVQSVADIFRVMSLGSGNDNVPGYSLSKMWVTGKELRNITEILLFT
                     SATASSNYPYYSHLRIEYDPEGGLFNKVRKIEITDRLGNVKEVNTDKDDPKLYSIVAN
                     SYMADNLGLVKKKTFGLIKVEPKDSNGNIVTEMDDFVMDFNDAQPGIQEGKEWIALLK
                     YLQRFKPAEEGGLPVIPDSYRNPVKSLVPVNRPK"
     gene            1899..2516
                     /locus_tag="PKK03_11720"
     CDS             1899..2516
                     /locus_tag="PKK03_11720"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNX85108.1"
                     /translation="MIIKARHIPFFVRFFSYYSRTGLKRHFGDVRFDCNTDTAGRPVL
                     LLGNHFSWWDGFIAYRLNDLFLHKKFHIMMLEEQLESRRFLNKAGAFSIKRGSRTVVD
                     TLKYTSGLLHDSANMVVVYPQGIITSVHRRPVRFERGTERIIAGASDKLMILFYVALP
                     DWFSEKKPGLYVRVIEYSARERSVTDLEETYNMFLDECIAKQIPV"
     gene            2513..3613
                     /locus_tag="PKK03_11725"
     CDS             2513..3613
                     /locus_tag="PKK03_11725"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX85109.1"
                     /translation="MILTVAGSLVLYLTFLRFAVALVNLVSRPYLPFVTRDRSRLPSL
                     SVLIPVRNEEKNIGTLLGSLSLLPYEKAEIIVYDDGSTDSTAEVIAACARVDRRIRCI
                     SGDELPEGWTGKNHACHRLAAEAANEYLLFLDADVTVGEDLLKRAVSYASRHRLALLS
                     MFPRQKMRSRGEKIVVPFMFRILLSLLPLFLIRRCRWTSFSAANGQMMLFHGETYRRY
                     RFHERVRDRLAEDIEIMRLVKRSRLRGDTLVGRKEIECRMYRSYIQGVNGFSRNIFSM
                     FGNSTTFFLLYAITGLAGWIILLFLPWQFAALYFMMVVLLNAMIAATSRQKIRENLLW
                     LLPGIAAFYHIAFLALRRRKHRTFEWKGRKIR"
     gene            3589..4359
                     /locus_tag="PKK03_11730"
     CDS             3589..4359
                     /locus_tag="PKK03_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85110.1"
                     /translation="MERKKNKMMQNVAVFIMMLLAMPAASGAATEDCSSCTIYKGYVS
                     GNMEIWKKGMQELQEQYRRDPKSCTLYTLAEARYGYIGYLLGRNEKDLARPQIETFDG
                     EVGQLARFPEYHAETEAFRVALLGFRMGLNPARAVTLGPKALKQLEAAMEAGKNSAVV
                     WIEKANSEAHMPAFAGGSKEKAAASFREALRLFEAGGSVAYCNWRYLNTMVLLGQLLE
                     KMGDNSGARDAYRMALRRAPDFQWVRDELLPEVEKKLK"
     gene            4364..>4535
                     /locus_tag="PKK03_11735"
     CDS             4364..>4535
                     /locus_tag="PKK03_11735"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNX85111.1"
                     /translation="MTGKKALIVGAGLGGLATALRLRRRGYEVEMVEMYRQAGGRLNQ
                     LTSGGFTFDMAPT"
BASE COUNT         1175 a         1057 c         1251 g         1052 t
ORIGIN      
        1 catccggagc ctcatctgtc cgggtgtatg aattctttca gcccctctgt ggcagggata
       61 taaagctttt tgctaactta atcccaaatt tcatgagaga tgaaaaggat gattattata
      121 gcaatattcc tcaccgtagc attggcgctc gcagcacagg aggaaaagaa actgaccata
      181 cttcatacaa atgattttca ttcgcatctc cagggctatg ccccggagtc tgcctataca
      241 cccggcgtgg ctgacaatga tcccacaatc ggaggaatgg cacgcattgc cggtattatt
      301 gaggcggtgc gtaatgagaa tcccggatca gcacttgtag ttgatgcagg tgactgtctt
      361 atgggaacgc ttttccaggc gctggagccg gagaccggat tccagcatgc gctgatgaaa
      421 aaagcgggat atgatgtggt tgccatgggt aatcatgatt tcgacttcgg gccggaggcc
      481 tttgccggca tactgagaaa agcgtcacag cgcggagaga taccccttat cctgacggga
      541 aatgccatca ctgatcccga tgaccctgct gatgatgcat tcgaggcatt gataagcgat
      601 ggtctcatta aaccttatac cgtaaaggag ataaacggca tcaggatagg ccttttctcc
      661 ctgatcggca aggacgcaga tgaatcagct ccctatgccc cgccggtgac ctttggcaaa
      721 ataatcccgg ctgcaaaaaa actggtcagg aatctgaaaa aggaaggttg tgatgttatt
      781 atatgtctgt cacacagcgg cataagaaaa gacgataaag gggaatggac aggtgaggat
      841 gtgaaacttg cctcaaaagt gaaagggatc gaccttatca tcagcgggca tacccatacg
      901 ctgcttaagg agccgcttgt tgtaaacgct gtaccgatag tagcggtcgg agacaacgga
      961 aggtttgtcg gaagaattga tgcccgggta atcaacggca gcttaaagct tgaaaaatac
     1021 gaggccattc ctgttgacga cagaaccact gccctggaag agatacaatc agaaattgta
     1081 ttacagcagc agaagatcaa cgaagcaata ttaaacccca tcgggatgac ctataccatg
     1141 ccggttgcca ttgcacccta cactgtcagc tatgatgaat acgggaaggc agctgacagc
     1201 aaccttggcg tccttgtggc agatgccata tacaactatg ttaatcagga aggccccggc
     1261 actgatatcg ccatcgttgc tgcaggcgtc ctgcgtgatc cgatccagcc tggcgtacag
     1321 agcgtggcgg atatcttcag ggtaatgtca cttggctccg ggaatgataa tgtgcccgga
     1381 tactcgctgt caaaaatgtg ggttacagga aaagaactca ggaatattac cgagatactc
     1441 ctctttacct cagccacagc ctcatcaaat tatccctatt attcccatct ccgcatagaa
     1501 tatgatccgg aaggagggtt gttcaacaaa gtgcgcaaga tcgaaatcac ggacagactg
     1561 ggaaatgtga aggaagtaaa taccgataag gatgatccga aattatattc aatcgtggcc
     1621 aattcatata tggcagataa ccttggcctt gtgaagaaga aaactttcgg tctgatcaag
     1681 gttgagccaa aagacagcaa cggaaacata gttaccgaga tggatgattt cgtgatggac
     1741 ttcaatgatg ctcagcccgg catacaggag ggcaaggaat ggatagcgct cctgaaatac
     1801 cttcagcgat tcaaaccggc tgaagagggc ggattgcccg tgattcctga ttcttaccgc
     1861 aatccggtaa aatcactggt tcccgtaaac aggccgaagt gattataaaa gcaagacata
     1921 ttccattctt tgtcaggttc ttcagttatt attcacggac cggactgaag cggcatttcg
     1981 gggatgtcag gtttgactgc aacactgata ctgccggccg gccggttctg cttctgggta
     2041 accacttcag ctggtgggac gggttcattg cctatcgcct caatgacctc ttcctccata
     2101 agaaatttca catcatgatg ctggaggaac agcttgaatc gcgtcgtttt ctgaataagg
     2161 caggggcatt ttcgataaag agagggtcac gcacagtggt ggacaccctg aaatatacct
     2221 caggcctgct acatgattca gccaacatgg ttgtggtata tcctcagggc ataattacct
     2281 cggtacacag gaggccggtg cggtttgaga gaggtaccga acgtatcata gcgggagcct
     2341 cggacaagct tatgatactt ttttatgtcg ccctgccgga ctggttctcg gagaagaagc
     2401 cggggctgta tgtcagggtc attgaatatt cagccaggga gagatcagtc actgaccttg
     2461 aagagacata caacatgttt cttgacgagt gtattgccaa gcaaatacct gtatgatact
     2521 gaccgttgcc gggagccttg tcctctacct tacctttttg agattcgcgg tggcgctggt
     2581 gaatctggtg tcccggcctt atctcccttt tgtcaccagg gacaggtcgc ggttgccgtc
     2641 gctttccgtc cttatacctg tcaggaacga ggaaaagaat attggcactc tgcttggcag
     2701 tctttcactg cttccttacg aaaaggctga gatcattgtg tatgatgacg gatcaacaga
     2761 cagcacggct gaggtaatag ccgcatgcgc gagggttgac cggagaatca gatgcatcag
     2821 cggcgatgag ctccctgaag gatggacggg taagaaccat gcctgtcaca ggcttgccgc
     2881 tgaagcggcg aacgagtacc tcctcttcct cgatgctgac gtgacagtgg gtgaagacct
     2941 gctgaagagg gcggtgtcat atgcatcaag gcacagactg gcgctgctct cgatgttccc
     3001 gcgccagaag atgagaagcc gcggagaaaa gatcgtggtt ccgtttatgt tcaggatact
     3061 tctttcactg ttgcctcttt tcctgataag gcgatgcagg tggacctcct tctctgctgc
     3121 caacggccag atgatgctct tccacggtga gacctacagg cgttaccggt tccatgaaag
     3181 agtgcgcgac aggctggcgg aggacatcga gatcatgagg ctggtgaaaa gaagcaggct
     3241 caggggagac accctggttg gccggaagga gattgaatgc cggatgtaca ggagttatat
     3301 ccagggagtc aacggattct cgcgtaacat tttttcaatg ttcggaaaca gcacgacctt
     3361 tttccttctt tatgcaatta ccggtcttgc cgggtggatt atactgctct tcctgccctg
     3421 gcagtttgcc gcgctgtatt tcatgatggt ggtgctcctt aatgccatga ttgctgcaac
     3481 cagcaggcag aagataagag aaaacctgct gtggctgctt cccggaatcg cagcttttta
     3541 ccatattgca tttctggctc ttagacggag aaaacacaga acttttgaat ggaaaggaag
     3601 aaaaataaga tgatgcaaaa cgttgcggta tttattatga tgttgctggc catgccggcg
     3661 gcatctggtg ctgccacgga agactgcagc agctgtacca tatataaagg atacgtttcc
     3721 ggcaacatgg agatatggaa gaaggggatg caggagttgc aggaacagta ccggcgtgat
     3781 ccgaagtcat gcacactata taccctggcc gaagcgagat acggatatat cggctacctg
     3841 ctgggcagga atgagaagga tctggcaaga cctcagatag agacatttga cggtgaggtc
     3901 gggcagcttg cgaggtttcc tgaataccac gcagaaactg aggctttcag ggtggccctg
     3961 ctcggtttca ggatgggact caatcccgca cgggcagtaa ccctcgggcc gaaggccctg
     4021 aaacagctcg aggcagcgat ggaagccggg aaaaattctg ccgtggtctg gattgaaaag
     4081 gcaaattctg aggcgcacat gcccgctttt gcaggcggat caaaggagaa ggctgctgcc
     4141 tcattccgtg aggcgctgag gctcttcgag gccggcggca gcgtcgccta ctgcaactgg
     4201 aggtatctca acaccatggt gcttctcgga cagttgcttg agaaaatggg cgataacagc
     4261 ggggcccgcg atgcttaccg catggctcta aggagagccc ctgactttca gtgggtgcgc
     4321 gatgaactgc tccctgaggt ggagaaaaaa ctaaagtgac catatgacag gaaagaaagc
     4381 gctgattgta ggcgccggac ttggcgggct ggcgacggca ctgcgtctaa gacgcagggg
     4441 ctatgaggtg gagatggtgg agatgtacag gcaggcaggc ggaaggctca accagctgac
     4501 ctccggaggc ttcacctttg atatggcccc cacct
//
LOCUS       DAOPXU010000165         4496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_24901_length_4496_cov_11.249043, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000165 DAOPXU010000000
VERSION     DAOPXU010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24901_length_4496_cov_11.249043"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..3716
                     /gene="nifJ"
                     /locus_tag="PKK03_11740"
     CDS             156..3716
                     /gene="nifJ"
                     /locus_tag="PKK03_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNX85112.1"
                     /translation="MATKKFITCDGNQAASHIAYMFSEVACIYPITPSSTMAEYVDEW
                     AAHGLKNMFGEEVKVVEMQSEAGAAGAVHGSLQSGALTTTYTASQGLLLMIPNMYKMA
                     GELLPGVFHVSARTVAAHALSIFGDHGDIYATRQTGFAMLATGSIQEIMDLAGVAHLS
                     AIKSRVPFLHFFDGFRSSHEIQKIEEMNIEEMKKLVDQDALAAFRQRALNPGKPVTRG
                     TAQNPDIFFQAKEASNRFYTNLDDTVNEYMEKISGITGRTYKPFTYYGAPDAENIIIA
                     MGSVTETIKEVIDYLAETKGEKLGLISVHLYRPFSAKYLFNVLPKSVKRITVLDRTKE
                     PGANGDPLYLDVKDVFYGNKVQPLILGGRYGLSSKDTTPSQMISVFENMKKEKPMNQF
                     TVGIVDDVTFRSLPQLPEINVSHKGTYSAKFYGIGSDGTVGANKNSIKIIGETTDKYC
                     QAYFAYDSKKSGGVTTSHLRFGDHPIRSPYLVNTPDFVACHVPAYLDKYDMLRGLKKG
                     GTFLLNSIWDTEETKSKLPDHMKKYLAENKVNFYIINATSIAEELGLGTRTNTIMQSA
                     FFKVSEVIPYKLAVDEMKHAVIKAYGKKGENVVKMNNDAIDRGGDVTRVEIPAEWAGI
                     EVAAVKEDTGKPAFIREVVDAINGLRGDDLPVSAFVGREDGTFPAGTAAYEKRGIAVN
                     VPEWQMDKCIQCNQCSYVCPHAAIRPFLLTDEELAAAPEGTVAKQGIGNTKSHKFKIQ
                     VSVFDCTGCGNCADVCPAKEKALIMKPLETQLAEADRWTYMHEKVGYKDTVVDKYTNV
                     KNSQFAQPLFEFSGACAGCGETPYIKAITQLYGDRMVIANATGCSSIYGGSAPSMPYT
                     VNRNGHGPAWANSLFEDNAEYGFGIMLGATRIRERLERLMKESLGSGLAPETVAAFNE
                     WIAEKENSKVSRAVSEKVIAACEKDKAPVCKEILELKSYLVKKSFWVFGGDGWAYDIG
                     YGGLDHVLASGENINVLVLDTEVYSNTGGQASKSTPAGAVAKFAASGKKIRKKDLGQM
                     AMTYGYVYVAQVAMGSSQNQYFKAIREAEEYPGPSLIIAYSPCINHGLRNGMGKTQQQ
                     AEDAVTAGYWHNYRFDPRLEAEGKNPFQLDSKEPDWSKFQDFLKSEVRYTSLLKDFPG
                     EADVLFKQAEENAKWRYNNYKRLSLMEYGTVPAETETKTE"
     gene            3839..4279
                     /gene="ruvX"
                     /locus_tag="PKK03_11745"
     CDS             3839..4279
                     /gene="ruvX"
                     /locus_tag="PKK03_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNX85113.1"
                     /translation="MGRALAIDYGRKRCGVAVTDPMRIIASPLLTVPSGEMEAFLREY
                     IPRENVTDVIIGYPVTVNNLPSESVKYIDPFIARFRKIFPEIPLHLADERFTSQMALQ
                     TMIEGGVSKSGRRDKGMADRISASIILQSWLTVQENKKQELKKR"
     gene            4276..>4496
                     /locus_tag="PKK03_11750"
     CDS             4276..>4496
                     /locus_tag="PKK03_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNX85114.1"
                     /translation="MTYPIFVYGHPVLRKVAEEIDRDYPDLDRFIEDMFETMYKSDGL
                     GLAAPQVGKSIRLFVIDGEPLAEDHPEMK"
BASE COUNT         1224 a         1124 c         1177 g          971 t
ORIGIN      
        1 gccatggagt tcctgcgtga ccgcatcacc cagacacgct caaacgaaga gttcctcatt
       61 tcaatgaaca gcgagtaaaa ctctcgtaaa aaatgagtac atttgcaatt tattttttta
      121 aaggttattt gatcaataat tacttataat cagaaatggc aacaaaaaaa ttcatcactt
      181 gtgacggcaa ccaggcagca tcgcacatag cctatatgtt cagcgaggtg gcctgcatct
      241 atcctattac tccttcctcc accatggcag aatatgttga tgagtgggca gcacacggac
      301 tgaagaacat gttcggtgaa gaggtaaagg ttgttgagat gcagagcgag gccggtgccg
      361 caggagcagt acacggttcg cttcagtcag gtgcactgac gacgacctac accgcatcac
      421 aggggcttct tcttatgatc cccaacatgt ataaaatggc aggcgaactc cttccgggag
      481 tatttcatgt cagcgcacgt acggttgctg cacatgcact ttcaatcttc ggtgaccatg
      541 gtgacattta tgccacccgc cagacaggtt tcgcaatgct ggccaccggc agcatacagg
      601 agataatgga ccttgctggt gtggcacacc tttcagcaat caagtcaagg gttcccttcc
      661 tccacttctt cgacggcttc cgttcatcac acgagatcca gaagattgag gagatgaaca
      721 ttgaggaaat gaaaaagctt gttgaccagg atgctctcgc agcattccgt cagagagctc
      781 taaatcccgg gaaaccggta accaggggaa cagctcagaa ccctgacatc ttcttccagg
      841 ccaaggaggc atcaaaccgt ttctatacca accttgacga tactgtcaat gagtatatgg
      901 aaaagatttc cggaatcacc ggtcgtacat acaagccgtt tacctattac ggtgcaccgg
      961 atgcagaaaa cataatcatt gcgatgggtt ctgtcacgga gactatcaaa gaagtcattg
     1021 actacctggc agaaacaaaa ggcgagaaac tcggactcat cagtgtccac ctctacaggc
     1081 cgttctcagc caaatacctc ttcaatgtcc tgccgaagag cgtcaaacgc attaccgttc
     1141 ttgaccgtac aaaggaaccg ggagccaacg gagatccgct gtatctcgat gtaaaggacg
     1201 tattctacgg taacaaggtg cagccgttaa tccttggcgg acgctacggt ctcagctcga
     1261 aggataccac tccctcacag atgatctcgg ttttcgagaa catgaagaag gagaagccta
     1321 tgaaccagtt cacggtagga atcgttgacg acgtaacctt caggtcgctg ccgcagcttc
     1381 ccgagatcaa cgtgagccat aagggcacct attcagccaa gttctacggt attggctctg
     1441 acggaacagt cggcgcgaat aagaactcga tcaagatcat aggtgagacc accgataagt
     1501 attgccaggc ttacttcgca tatgactcaa agaaatcggg cggtgtgaca acatcacatc
     1561 tccgtttcgg tgatcatccc atccgttcgc cctatcttgt caacactccc gatttcgtgg
     1621 cgtgccacgt accggcatac cttgacaagt atgacatgct caggggcctc aagaagggag
     1681 gcaccttcct cctgaactcc atatgggata cagaggagac gaagagcaag ctgcctgacc
     1741 atatgaagaa gtatctcgca gaaaacaagg tcaacttcta catcatcaat gcaaccagca
     1801 ttgctgaaga actgggcctc ggcaccagga caaacaccat catgcagagc gccttcttca
     1861 aggtatcgga agtaattccg tataagcttg ctgttgacga gatgaaacat gccgtcatca
     1921 aggcttatgg caaaaagggc gagaacgttg tgaagatgaa caatgacgct attgatcgcg
     1981 gtggtgacgt taccagggtt gaaattcctg cagagtgggc cggcatagag gtagctgcag
     2041 tgaaggaaga caccggcaag cctgcattca tccgcgaggt agttgatgcc atcaatggtc
     2101 tcaggggcga tgatcttcct gtcagtgcgt ttgtcggacg cgaggatggc acattccctg
     2161 caggaacggc ggcatatgaa aagcgtggaa ttgctgtcaa cgtgcccgag tggcagatgg
     2221 acaagtgcat acagtgcaac cagtgctcat atgtatgtcc tcacgccgca atccgaccct
     2281 tcctcctgac tgacgaggag ctggcagcag cgcctgaagg tactgttgca aaacagggta
     2341 ttggaaacac caagagccat aagttcaaga tccaggtaag tgtatttgac tgcaccggct
     2401 gcggcaactg tgccgatgtt tgtcccgcca aggaaaaggc ccttatcatg aagcccctgg
     2461 aaacacagct tgcagaagct gacaggtgga cctacatgca tgagaaggta ggatacaagg
     2521 atactgttgt tgacaagtac accaacgtca agaactctca gtttgcgcag ccgctctttg
     2581 agttctcggg agcatgtgcc ggatgcggcg agactcctta catcaaggcc atcacccagc
     2641 tttacggtga ccgcatggtc atcgccaacg ccacaggttg ttcatccatc tatggtggtt
     2701 cggctccttc aatgccttac acggtgaaca ggaacggtca cggtccggca tgggccaact
     2761 cactgttcga agacaacgcc gagtatggtt tcgggattat gcttggcgcc acccgcatca
     2821 gggagcgtct tgagcgcctg atgaaggaga gtctcggaag cggacttgca ccagagaccg
     2881 ttgcggcatt caacgagtgg attgccgaga aggaaaactc aaaggtcagc agggctgttt
     2941 cggagaaggt tatcgcagca tgcgaaaagg acaaggctcc tgtttgcaag gagatccttg
     3001 agctgaagag ttacctggtc aagaagtcgt tctgggtatt cggcggtgac ggatgggctt
     3061 atgacatagg ctacggcgga ctcgaccatg tccttgcctc aggcgaaaac atcaatgttc
     3121 tcgtgcttga cactgaggtc tactccaata ccggaggcca ggcttcgaag tcgacacctg
     3181 caggtgctgt tgccaagttt gccgcttcgg gcaagaagat ccgcaagaag gacctcgggc
     3241 agatggccat gacctatgga tatgtttatg tagcgcaggt tgccatgggc tcaagccaga
     3301 atcagtactt caaggcaatc cgtgaagcag aggagtatcc cgggccgtca ctcataatag
     3361 cctactcacc ctgtatcaac cacggcctcc gcaatggcat gggcaagaca cagcagcagg
     3421 ctgaggatgc agtaaccgca ggatactggc acaattacag gttcgatccg cgccttgagg
     3481 cagagggaaa gaatccgttc cagctcgact ccaaggagcc ggattggagc aagttccagg
     3541 atttcctcaa gtcagaggta cgttacacat cgcttctgaa agacttcccc ggcgaggctg
     3601 acgtgctgtt caaacaggca gaggagaacg caaaatggag atacaacaac tataagcgtc
     3661 tttccctgat ggagtacggc accgtacccg cagaaactga aaccaaaaca gaataaagct
     3721 gttatatgac gaagggagag ccggcgctga ccggctctct tctttttatg gaggaaaacc
     3781 ggtatctttg cgcccggcag aataccggct gaacagaccg gaacaaagcc ggaacagaat
     3841 gggcagagca ctggcaattg actacggaag gaagagatgc ggagtggcag tcaccgatcc
     3901 gatgaggatc atcgcttctc ccctgctgac tgtgccatca ggagagatgg aggcttttct
     3961 gcgcgaatac atcccgcggg agaacgtaac tgacgtcatc atcggttacc cggtgaccgt
     4021 gaacaatctg cccagtgaaa gcgtaaaata tattgatccg tttattgccc ggttcaggaa
     4081 gatctttccg gagatacccc tgcacctggc tgatgaacgg ttcacttcac agatggcatt
     4141 gcagacaatg attgaaggag gagtaagcaa aagcggaagg cgcgacaagg gcatggctga
     4201 ccgtatcagc gcttccatta ttctccagtc atggctgaca gtccaggaaa acaaaaaaca
     4261 ggaactgaaa aagagatgac ttaccctatt tttgtatacg gacaccccgt cctgagaaag
     4321 gtggcagagg aaatagacag agattaccct gaccttgaca ggttcattga agatatgttc
     4381 gaaaccatgt acaagtctga cggtcttggc cttgctgccc cacaggttgg taagtcaatc
     4441 aggcttttcg tcattgacgg tgagcctctg gccgaggatc accccgagat gaagga
//
LOCUS       DAOPXU010000166         4468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_25121_length_4468_cov_12.948108, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000166 DAOPXU010000000
VERSION     DAOPXU010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25121_length_4468_cov_12.948108"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PKK03_11755"
     CDS             <1..345
                     /locus_tag="PKK03_11755"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNX85115.1"
                     /translation="LIMPYREKKVEKLFYQIGEVAEMFSEPVSTIRFWEKEFDILKPR
                     KNNKGNRLFMQEDLKNLRLIHHLLRERGMTLDGAKKYLKNNRDDADYRLEIADTLRNI
                     RAMLVEIRDEIK"
     gene            350..1477
                     /locus_tag="PKK03_11760"
     CDS             350..1477
                     /locus_tag="PKK03_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNX85116.1"
                     /translation="MAVTLAEFVRWLEGLIPPSFQEEYDNSGLQVGDLTAEVASVLLT
                     VDVTPDVIDEAAAGGFDLVLSHHPLIFRPLKRLAYGSNSETCVASAIMKGIAVYSAHT
                     SFDNAGNGVSRILAEKIGLEGIRVLEPLRGRLLKLVTFVPESHSVAVREALHAAGAGN
                     IGNYDSCSFNTRGEGTYRAGEGADPFAGGPGEFHTESEVRIEVVLPSHIKGACLKAML
                     AAHPYEEVAWDLIALENEYRGAGAGAIGALAAPLTGAALLERLRALTGLPVIRYCGDP
                     ARTVTTVAVCGGAGSHLISSAARTGADAFITGDIKYHTFTEAPGEMLVADIGHYESEK
                     FSLEILYDLIQKKFPNFALRFSGIKTNPINYYGYDKTDEIT"
     gene            1452..2219
                     /locus_tag="PKK03_11765"
     CDS             1452..2219
                     /locus_tag="PKK03_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNX85117.1"
                     /translation="MTKQTKSPETELTIEEKLRALYSLQLVDSEIDKIRTLRGELPLE
                     VQDLEDEVAGLETRINNLKSEVSELEKSVVGKTNEITASQALIKKYEEQQNNVRNNRE
                     YDSLSKEIEFQTLEIELCNKKIREFTAQVAEKKEVMAEAQTMLEERKRDLESKKSELE
                     DITHDTQKEEEQLASRSGELQTRIEDRLLTAYKRIRTNARNGLAVVPVKRDACGGCFN
                     QIPPQRQLDIRSRKKIIVCEYCGRILVDDEIVGEANQ"
     gene            2394..4406
                     /locus_tag="PKK03_11770"
     CDS             2394..4406
                     /locus_tag="PKK03_11770"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNX85118.1"
                     /translation="MNDFKSQIREGIPSNLPAPKAWDPAVNHAPRRRDILTGEEKKLA
                     LRNALRYFPPSLHGELAPEFAAELRERGRIYMYRYRPDYEMKARPISEYPHRSAQAAA
                     IMLMITNNLDPAVAQHPHELITYGGNGAVFQNWAQYRLTMKYLSEMTDEQTLVMYSGH
                     PLGLFPSHREAPRVVVTNGMVIPNYSSQDNWERFNALGVSQYGQMTAGSYMYIGPQGI
                     VHGTTITLLNAQRRVKPVAAEERRSGLFISSGLGGMSGAQPKAGNIAGVVSVIAEVNP
                     KAIRTRHTQGWVDEVHTCLSELAVRVRKACGDGEVVSLAYQGNIVDLWERFADEGLWA
                     DLGSDQTSLHNPWAGGYYPAGISFDEANRLMAEDPEKFRGLVQGSLRRQVEAINRHTA
                     HGTYFFDYGNAFLLEASRAGADIGNGRGGFRYPSYVEDIMGPICFDYGFGPFRWVCCS
                     GDPADLEKSDAMAAATLERLMKESPEDIRQQMSDNIHWIREAGKNRLVVGSQARILYA
                     DAAGRIMIASAFNDAIASGIISGPIVIGRDHHDVSGTDSPFRETSNIYDGSRFTADMA
                     MQNVIGDAFRGATWVSIHNGGGVGWGEVINGGFGMLLDGSPETSRRIRSMLLWDVNNG
                     IARRSWARNSGAMAAITREMERTPALVVTMPQEADQNLIDEIFRNS"
BASE COUNT         1088 a         1144 c         1304 g          932 t
ORIGIN      
        1 ctgatcatgc cttacaggga aaagaaagtg gaaaagctct tttaccagat cggtgaggtt
       61 gccgaaatgt tttcagagcc tgtttccacc attcgcttct gggaaaagga gtttgatatt
      121 ctcaaaccca ggaagaacaa caagggcaac cggctgttca tgcaagagga cttaaagaac
      181 ctcaggctga tccatcacct gctgagggag aggggcatga ccctggatgg ggcgaagaaa
      241 tacctcaaaa acaatcgtga cgatgctgat tacaggcttg agatagcaga taccctccgc
      301 aacatcaggg ccatgcttgt ggagatcagg gacgaaatca agtgatttta tggcggttac
      361 actcgcagaa tttgtaaggt ggctcgaggg gctgatccct ccgtcatttc aggaagagta
      421 tgacaacagc ggactgcagg tcggtgatct gacggctgag gttgcttctg tgctgctgac
      481 cgtcgatgtt acccctgatg taattgatga ggcggctgcg ggagggtttg atcttgtgct
      541 ctcacatcac cctctgatct tcaggccgct gaagcggctg gcttacggga gcaacagcga
      601 gacgtgcgtg gcatcggcga tcatgaaagg catagccgtt tattctgcac ataccagttt
      661 tgacaacgca ggcaatggtg taagccgtat tctggctgaa aagatcgggc tggaggggat
      721 aagagtgctg gagccgctga ggggcaggct gctgaagctt gttacctttg tgccggagtc
      781 tcattccgtg gctgtgcgtg aagccctcca tgccgccggt gccgggaata tcggaaacta
      841 tgactcgtgc agcttcaata caaggggtga ggggacatac cgtgccgggg agggagctga
      901 tccttttgcg ggcggcccag gtgagtttca cactgagtcc gaggtgcgta ttgaggttgt
      961 gttgccgtca catattaagg gagcctgtct gaaggccatg ctggcagcac atccctatga
     1021 ggaggttgca tgggatctga ttgcgcttga gaatgagtac aggggcgccg gcgcgggcgc
     1081 cattggcgcc ctggcagctc cgctgacagg cgcggcgctg ctggagcggc tgcgggcgct
     1141 caccggcctg cccgtcatcc gctactgcgg tgaccccgcc cgcactgtaa ccacggtggc
     1201 cgtatgcggg ggagcagggt cacacctcat cagcagcgcc gcgcgcaccg gcgccgacgc
     1261 attcatcacg ggagatatca aatatcatac tttcaccgaa gcgcccggag aaatgctcgt
     1321 ggctgacatc ggacactacg aaagtgaaaa attttcactg gaaatcctct atgacctcat
     1381 tcagaaaaaa tttcctaact ttgcactccg gttttccggg ataaaaacca atcccattaa
     1441 ttactacggg tatgacaaaa cagacgaaat cacctgaaac agaacttacc atagaagaga
     1501 aactgagagc tctctattca ttgcagcttg ttgattcaga gatagataaa atccgcaccc
     1561 tgcgcggcga actgcctctc gaggtgcagg accttgagga tgaggtggca ggacttgaaa
     1621 ccaggataaa caatcttaaa agcgaagttt ccgaactgga aaagtctgtc gttggcaaga
     1681 ccaacgagat aactgcctca caggcgctta tcaagaaata cgaggagcag cagaacaacg
     1741 ttcgcaacaa ccgcgagtat gactcccttt caaaggagat agaattccag accctggaga
     1801 tagaattgtg caacaaaaag atccgtgaat tcaccgccca ggttgcagag aagaaggagg
     1861 tgatggctga ggcacagaca atgcttgaag agcggaagcg cgacctggag agcaagaagt
     1921 ctgagctgga ggacataacc catgacaccc agaaggagga ggaacagctt gcatccaggt
     1981 caggagagct tcagacgaga atagaggaca ggctgctgac ggcttacaag cgtatacgca
     2041 caaatgcccg taacggcctc gcggtggtac cggtaaagag ggatgcatgc ggcggatgct
     2101 tcaaccagat tccacctcag cgccagcttg acatcaggtc aaggaaaaag atcatcgtct
     2161 gcgagtactg cggccgcata cttgttgatg atgagatcgt gggagaagcg aatcaatagt
     2221 cggcagtacc agtttgagac tccggcgaag tcggaggatc aatcccgcat ccgccggccg
     2281 gcggatcggg acggcagtaa cagaaaccat tgtaaatgat atagagaggg acagcggaag
     2341 gtgtctctct ctctgtttta ttatctttac aacactgaat tttatccaat atcatgaatg
     2401 atttcaaatc acagatccgt gaaggcatac catcgaacct tcctgcaccc aaagcctggg
     2461 atcccgcggt gaatcatgcc ccgcgccgcc gtgacattct gaccggtgag gagaaaaagc
     2521 ttgcccttcg caacgccctg aggtattttc ccccgtcgct tcacggtgaa ctggctcctg
     2581 aatttgcagc cgaactccgt gagcggggac gtatatatat gtatcgttac aggcctgact
     2641 atgagatgaa agcccgtccg ataagcgaat atccgcaccg gtcagcgcaa gctgccgcaa
     2701 taatgctgat gatcaccaac aacctcgatc ctgccgttgc ccagcatccg cacgagctca
     2761 tcacctatgg cggcaacgga gctgtttttc agaactgggc tcaatacagg ctcaccatga
     2821 agtacctctc tgagatgacc gatgagcaga cgctggtgat gtactcggga caccccctcg
     2881 gcctctttcc ttcgcacagg gaggcgcctc gggtagttgt caccaacggt atggttatcc
     2941 ccaactattc gtcacaggat aactgggagc ggttcaatgc tctcggggta tcacagtacg
     3001 ggcagatgac cgccggctca tacatgtata tcggtccgca gggaatagtt cacggtacca
     3061 ccatcaccct cctcaatgca cagcggcggg ttaagccggt tgcggctgaa gaacgcagga
     3121 gcgggctctt catctcgtcc ggacttgggg ggatgtcagg ggctcagccc aaggctggaa
     3181 atatagccgg ggttgtgtca gtcattgccg aggtcaatcc gaaggctatc aggacacgcc
     3241 acacccaggg atgggttgat gaggtacaca cctgtctgtc agagctggct gtgcgggtcc
     3301 gcaaggcctg cggtgacggc gaggtggtgt cactggccta ccaggggaac atcgtcgacc
     3361 tgtgggagag gtttgctgat gaaggtctgt gggctgacct gggatcggat cagacctcgc
     3421 ttcataatcc gtgggcagga ggttactacc ctgcagggat cagctttgac gaggcaaaca
     3481 ggctcatggc tgaagacccg gagaagttcc gcgggctggt gcagggttcg ctccgccggc
     3541 aggtggaggc catcaacagg catacggcac acggaaccta tttttttgat tacggcaatg
     3601 ccttcctcct ggaggcttca cgtgccggcg ccgacatagg caacggcagg ggagggttcc
     3661 gttacccgtc gtatgtcgag gatatcatgg gacccatctg cttcgactac ggcttcggcc
     3721 cgttccgctg ggtttgctgt tccggtgacc ctgccgatct ggagaagagt gacgccatgg
     3781 ctgctgccac ccttgagcgc ctcatgaaag agtcgcccga agatatccgt cagcagatgt
     3841 ctgacaatat tcactggatc cgcgaggcgg gaaaaaaccg cctggtggtt ggatcccagg
     3901 cgcgtatcct ttatgccgac gccgccggca ggataatgat agcctctgcc ttcaatgatg
     3961 cgatcgcttc ggggatcatc agcggcccga tagtgatcgg tcgtgaccat cacgatgtgt
     4021 caggcacaga ctcaccgttc cgtgagacat cgaacattta tgacggttca cgtttcacgg
     4081 ctgacatggc aatgcagaat gtcattggcg acgctttccg cggggccacg tgggtctcca
     4141 ttcacaatgg cggcggcgtg ggctggggcg aggtgatcaa cggcgggttc gggatgcttc
     4201 ttgacggcag tccggagaca tcacgccgca tccggtcaat gttgctctgg gatgtcaaca
     4261 acggcatcgc acgtcgcagc tgggcgcgca acagcggcgc aatggccgcc ataacaaggg
     4321 aaatggaacg caccccggct ctggtggtca ccatgccgca ggaggcagat cagaacctca
     4381 tagatgaaat tttcaggaat agctgataaa attgcgattt gcgatttccg aattaaaaaa
     4441 tcgaaaatcg gcaatagtga aaaggacc
//
LOCUS       DAOPXU010000167         4351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_26125_length_4351_cov_11.836359, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000167 DAOPXU010000000
VERSION     DAOPXU010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26125_length_4351_cov_11.836359"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PKK03_11775"
     CDS             complement(<1..606)
                     /locus_tag="PKK03_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85119.1"
                     /translation="MKRNFFAVGMIAALVFGASGNLSGIDTSDTRMLSQPAASKTHVA
                     FIYAEDLWVMNADGTDPRRLTVDEGIEGEPCFSPDGKLIAFSAQYDGNTDVFVIPVEG
                     GVPVRLTWHPGADVARTFTPDGAKVVFASQRSTFTGRYAQLFTVGIEGGYPEQMQIPN
                     AHYVSLSPDGKRMAYTPLPPAHAQWKNYRGGTVSTIWLFTQA"
     gene            complement(609..1373)
                     /locus_tag="PKK03_11780"
     CDS             complement(609..1373)
                     /locus_tag="PKK03_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX85120.1"
                     /translation="MQKTVLITGATSGIGEAAAVKFAESSDRLIITGRRKERLDEFAD
                     HLRDTYGAEVLALCFDVRNRHEVEEHLGALREEWKKIDILINNAGLAVGLNHIDEGDT
                     DDWDRMIDTNVKGLLYVTRVVSPLMVARGTGHIFNIASIAGKEVYENGAVYCASKHAV
                     DALSRAMRIDLLKHGIKVTNIAPGMVETEFSEVRFKGDTDRAAAVYTGLEPLTGDDIA
                     GVIHYCASLPDHVCINDLVITPKAQANATNTFRKTR"
     gene            1525..2271
                     /gene="ubiE"
                     /locus_tag="PKK03_11785"
     CDS             1525..2271
                     /gene="ubiE"
                     /locus_tag="PKK03_11785"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNX85121.1"
                     /translation="MQNTTGNSGPSADKVESMFDSISKHYDRLNHLLSFGTDRFWRRR
                     AVSLIGRHIKPVKMLDVATGTGDLAIEALRLRPEKITGIDISSRMLEEGLRKINDMGY
                     GGTIELLRGDASATGFSDGTFDVVMSAFGVRNFENTLTGLTEMCRVVRPGGMIMVLEF
                     SRPEWFPFRQIYGFYFRRILPLIGRKVSGDRSAYSYLPDSVMAFPDNEKFLVLLEKAG
                     FSRVAQKRLTGGIASIYTGFRNGEGQYNKN"
     gene            2307..3005
                     /locus_tag="PKK03_11790"
     CDS             2307..3005
                     /locus_tag="PKK03_11790"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNX85122.1"
                     /translation="MYKRTAIIILLLLMPLLLVAQKAKPKNDSNYDDRLLHFGFSMGI
                     NTMDFIVNNKAESDLKAEIVSLKPGINIQIVTDYRPSTYLDLRFLPGVSFGQRNINFY
                     DLSGAKWGETQVIESSFLEFPLSLKAKGMRLNNSRPYLLAGVNFRYDLAGKREYDIDR
                     PVYIRLKRPDLYYEVGAGIDFYLPYFKLTIEAKMSTGLFDVMVHEPFPGFEEYVTSIG
                     SLRSQIWSLSFHFE"
     gene            3008..>4351
                     /locus_tag="PKK03_11795"
     CDS             3008..>4351
                     /locus_tag="PKK03_11795"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNX85123.1"
                     /translation="MSGELNLVVPPQTAFDETALRRFLEKKSGRNDFTIRILRRSVDA
                     RKPEIKVNLTVQLGYPGEEQEVTEPFRITDVSHAREVLIAGSGPAGLFAALALIEAGL
                     KPVILERGREVHERKRDVAAISRQHIVDPDSNYCFGEGGAGTFSDGKLYTRSKKRGDN
                     RRVLELLVLHGADPAILYEAHPHLGTDKLPGIITAIRNTIREAGGEVLFGKRITGISV
                     SDGRFREVTANGNETFRAGDLILATGHSARDIHAMLAGAGVKLAFKPFAMGVRVEHPQ
                     ELIDRIQYHGRPRGEYLPAAAYNLVTQISGRGVYSFCMCPGGFMVPSATSPEEVVVNG
                     MSPSNRNSRWANSGIVTEIREEDAIRYGSGPLAGTAYQAALEKMAWKEGGMTQRAPAQ
                     RLTDFVEGRASSTLPDASYFPGLTASPLHQWMPADISGRLREGFREFGKKMRGFLT"
BASE COUNT         1076 a         1091 c         1180 g         1004 t
ORIGIN      
        1 ggcctgggtg aagagccaga tggtcgaaac ggttcctccg cggtagtttt tccactgggc
       61 atgtgccgga ggcagcgggg tgtaggccat ccttttccca tcgggagaca gggaaacata
      121 gtgagcgttt ggaatctgca tttgttccgg gtaaccccct tcaattccca ccgtgaagag
      181 ctgggcatag cggccggtga atgtcgacct ctgtgaggcg aagaccacct tcgccccgtc
      241 aggggtgaat gttcgtgcaa catcagcccc cgggtgccag gtgagcctaa ccggcacacc
      301 gccctccacc gggatgacaa agacatccgt gttcccgtca tactgggcac tgaatgctat
      361 aagcttcccg tcaggggaga aacacggttc gccttcaatt ccttcgtcaa ctgtgagacg
      421 gcggggatca gttccgtcgg cgttcatcac ccagagatcc tcggcgtaga taaatgcgac
      481 atgtgtttta ctggctgcgg gctgtgacag catccgtgtg tcagatgtgt caataccgct
      541 aagattgcct gatgctccga aaacgagagc tgcaatcatt ccaactgcaa agaaatttct
      601 tttcatggct atctggtttt tctgaaagtg ttggttgcgt ttgcctgtgc tttgggtgtg
      661 atgaccaggt cattgataca aacatgatcg ggaaggctgg cacagtaatg gatgaccccg
      721 gcaatgtcat cgccggtgag cggctcgagg ccggtataaa cggctgcggc cctgtcagtg
      781 tcacccttga agcgtacttc cgagaactcg gtctcaacca tgcccggtgc aatgtttgtc
      841 acctttatgc catgcttcag aaggtcaatc cgcatggccc tcgagagagc atcaacggca
      901 tgcttggagg cacagtatac ggcaccgttc tcgtatacct ccttcccggc aattgatgcg
      961 atattgaaaa tgtgacccgt gccccttgcc accatcagcg gcgataccac ccgggtaacg
     1021 taaagaagcc cctttacatt cgtgtcaatc atcctgtccc agtcatcggt gtctccctcg
     1081 tcaatatggt tcagacccac tgccagcccg gcattattga tgagaatgtc aatcttcttc
     1141 cactcctccc taagggctcc gagatgctct tccacttcgt gcctgttcct cacatcgaaa
     1201 cagagagcaa gcacttcagc cccataagta tcccggaggt gatcagcgaa ttcatccagt
     1261 ctttcttttc ttcttcctgt aataatcagt ctgtcagatg attctgcaaa cttcacggct
     1321 gcggcttccc cgatacctga agtcgcacct gtaatcagta ctgtcttttg catagcataa
     1381 cttattcaga tgggtaaatg taaaaatatt aagggtatct ttgccgcctg tccggaaaat
     1441 gatgggttaa atctaccata aagacacatg gatgtccggc agtgatgcaa gaaaaaacag
     1501 ccagctgaca aaaaatctgt actgatgcaa aacacaaccg ggaacagcgg accatctgca
     1561 gacaaggttg aaagtatgtt tgattcaatc tcaaagcatt atgaccggct taaccatctt
     1621 ctttcctttg gcaccgacag gttctggagg aggagggctg tttcgctcat cggcaggcat
     1681 attaaaccgg ttaaaatgct cgatgttgcc acgggtacag gagacctggc catcgaggcg
     1741 ctcaggctca ggcctgaaaa gattaccggc attgacatca gctccaggat gctggaggaa
     1801 ggcctccgga agattaatga catgggctat ggaggcacca ttgagctgct gaggggcgat
     1861 gcctctgcca ccggattcag cgatggaact tttgatgttg tgatgtcagc gttcggggtg
     1921 cgtaattttg agaacaccct gaccggactg accgagatgt gccgcgtggt caggcccgga
     1981 ggaatgataa tggtgctgga attctcaagg ccggaatggt ttcccttcag gcagatttac
     2041 ggattttatt tcagaaggat tctgcccctt ataggaagaa aggtttcggg tgacaggtca
     2101 gcctattcct atctgcccga ttcggtgatg gcattcccgg acaatgaaaa atttctcgta
     2161 ctgcttgaga aggccggatt cagccgcgtt gcacagaaga ggctgactgg aggcattgca
     2221 agcatatata ccggattcag aaacggcgaa gggcaataca ataaaaacta aattttccgt
     2281 tttattattc aggaccattc agaagagtgt ataaaagaac tgccataatc atccttctgc
     2341 ttctgatgcc attattgttg gttgcccaga aggcgaagcc caaaaacgac tcgaattatg
     2401 acgacaggct tctccatttc ggcttcagca tgggcatcaa cactatggat ttcatagtga
     2461 acaacaaggc agagtctgac ctgaaggcag aaatcgtcag cctgaagccg ggtataaata
     2521 tccagatcgt aacagactac agaccatcaa catatcttga cctgaggttt ctgccgggag
     2581 tatccttcgg gcagcggaac attaatttct atgacctgag cggagcaaag tggggagaaa
     2641 cacaggttat cgaatcatca ttccttgaat ttcctctttc acttaaggca aagggaatga
     2701 gactgaacaa ttcaagacca tacctgctgg ccggggtaaa tttccgttat gacctggccg
     2761 ggaaaaggga gtatgatatt gacaggcctg tttatatcag gctgaaaaga cccgatcttt
     2821 actatgaggt tggcgcgggc atagactttt accttcctta tttcaagctg accattgagg
     2881 caaagatgtc tacaggactc tttgacgtca tggttcatga acctttcccg gggttcgaag
     2941 agtatgttac ttcgatcgga tcactcaggt ctcagatctg gtctctctcc ttccattttg
     3001 aatagtcatg tccggggaac taaacctggt ggtgcctccg cagacagcct ttgatgagac
     3061 ggcactgagg aggttcctcg aaaaaaagag cggcagaaac gattttacca tccgaatact
     3121 caggcgttca gtcgatgcgc gtaagcctga gatcaaagtc aatctgactg tacagctggg
     3181 atacccggga gaggaacagg aggtgacaga accgttcagg atcaccgatg tctctcatgc
     3241 cagggaagtg cttatcgccg gcagcgggcc ggcgggactg tttgctgcgc ttgcactcat
     3301 cgaggccgga ctgaaacccg ttattcttga acggggcaga gaggtgcatg agcgaaagag
     3361 agacgtggca gccataagca gacagcatat cgttgacccg gactcaaatt actgtttcgg
     3421 tgagggtggc gcaggcacct tttccgacgg caagctctat acaagatcca aaaagcgggg
     3481 cgacaaccgg cgcgtgctgg aactgctggt actgcacgga gccgatcctg caatactgta
     3541 tgaggcacat cctcacctgg gaacggacaa actgcccgga ataatcaccg caatccggaa
     3601 taccatcagg gaagccggag gagaggtgct tttcggcaaa cgaataacgg gcatttcagt
     3661 cagtgacgga cggttccggg aagtgaccgc aaacgggaac gaaaccttca gggcaggtga
     3721 tctgatcctg gccacaggcc attcggcccg tgacatacat gcgatgcttg ccggagcagg
     3781 ggtaaagctt gcctttaagc ccttcgccat gggcgtaagg gtcgagcatc cccaggagct
     3841 gattgaccgc attcagtatc atggcaggcc acggggcgaa tatctgccgg cagcagcgta
     3901 caacctggtc acgcagattt ccggcagggg cgtatactct ttctgcatgt gccccggagg
     3961 attcatggtt ccttcggcca cttcgccgga agaggtggtg gtgaacggaa tgtcgccctc
     4021 aaaccgcaac tccaggtggg ccaacagcgg gatagtgacc gagatcaggg aggaggatgc
     4081 tatccgttac ggcagcgggc cgctggcagg cacagcatac caggcagccc tggagaaaat
     4141 ggcatggaag gaggggggaa tgactcagcg ggctccggcg cagaggctga ccgacttcgt
     4201 ggagggcagg gcttcatcca cactccccga tgcgtcatat tttcccggcc ttacagcctc
     4261 accgcttcat caatggatgc ctgcagatat ttccggaaga ctgagggaag gattcaggga
     4321 gttcgggaaa aagatgaggg gattcctcac c
//
LOCUS       DAOPXU010000168         4266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_26959_length_4266_cov_7.426502, whole genome shotgun sequence.
ACCESSION   DAOPXU010000168 DAOPXU010000000
VERSION     DAOPXU010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26959_length_4266_cov_7.426502"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1863)
                     /locus_tag="PKK03_11800"
     CDS             complement(85..1863)
                     /locus_tag="PKK03_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85124.1"
                     /translation="MKAFLLTVILATITAAGASQSLPGFKSSGQFDEQQTVIENSVAG
                     TRVLINAPMTGFGTDDRVLLVLYALPNGNTIEQTFGKLLKEDDDWHYDIQHIGAQTRF
                     LRRVITDRTVVVAYLENEQRSWPAWSANTPGHTEIVKKMVDEITSLFAPWNPELVLNG
                     HSGGGRFIFNYIDAHEQIPGCVVRIAFLDSNYGWEGERYGPKIVSWLRSGKSRYLCTQ
                     AYNDSVVIYNGKPLVSPEGGTWYRSWKMKDYLSASFSLKRRERDSLLWYSSRDRRIEF
                     IFKPNPGGKIFHTQQVEYNGLIHSILSGTRNEQKGYRYFGGRAYSGLISDTIIVPVRR
                     LNIPERNPAAEPGSEFMKRISELPLKDREEEIYIAAASGNIPAFLRETVTLKGVFNDL
                     NGTPHDLEYEVMPDYLAIGSDDDFCRIPITPGTAQRLADLFGASLLTAKLSDHVWSKA
                     EVKPEPFFYRPVGNANELVTKFEEHNEQIERQLADAGWRHGQLVAGIKKDVIISSRIA
                     ERPDRVVIYGWHRPDGSPIQPVYSGHIWWYVDYSHGIRFINARVLVDGIPVKVSDLLK
                     DQVLFRILTDEENPIEMSFYPKNRIL"
     gene            complement(1860..3212)
                     /locus_tag="PKK03_11805"
     CDS             complement(1860..3212)
                     /locus_tag="PKK03_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HNX85125.1"
                     /translation="MRRIIAILFFLAAGILVNAQLPAFPGAEGFGRYTTGGRGGAVYA
                     VTNLADSVVKPPEGSLRWALNKPSPKTIVFAVSGTIDLKRRLNISKGDVTIAGQTAPG
                     DGICIKGETVSIEADNVIIRHVRFRCGNEIAGEEPKDAISCIRHRNIIIDHCSMSWSV
                     DEAASFYDNENFTLQWCIISESLYDAGHPKGEHGYGGIWGGKGASFHHNLLASHTSRL
                     PRFCGARYHPDTRETELVDFRNNVIFNWGFNSSYGGEMGQQNMVNNYYKPGPATKKDV
                     ISRIVEPWDTVGRWHVSGNRIEGSSKVSSDNWSGGVQGDNASNPVIRAGEPFVVAAVR
                     TTSARRAYRSVLRNAGATLPHRDPVDHRIINETLAGYCAYGDSYGAGTGIIDTQNSAG
                     GWPLLKTYDVPADSDGDGMADSWELKKGLNPADPEDRNNMAGPGYTMLEEYINGLCKR
                     "
     gene            complement(3209..>4266)
                     /locus_tag="PKK03_11810"
     CDS             complement(3209..>4266)
                     /locus_tag="PKK03_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HNX85126.1"
                     /translation="GRAIEQSEMNVPLPMEGGIEAYEWMRDFLRPVMISLISCKKILI
                     DGPVFQNSPAWCIHPLMCQDLTVRNITVRNPWYSQNGDGIDIESCRNTLLYNCNFDVG
                     DDAICIKSGKNEDGRKRGIPTENLVIRNCIVYHGHGGVTIGSEMSGGVRNVSVAGCTF
                     MGTDVGIRFKSNRGRGGVVENIWFNDILMTDIPSQAISFNLYYGGLSVSEMLAEGKNI
                     ETTVGDIPPVTEETPQFRNISMKDITCRGADQAIYLQGLPELNLENITMENIDMTAVE
                     GMACIDARGIKIRNMRLVTEKKPLIFFLNSSDADVTGLQVAEDADGLVEVRGSSSARI
                     TVETVTPDGTARARTLR"
BASE COUNT          965 a         1215 c          996 g         1090 t
ORIGIN      
        1 ccgataagaa gagaaacgag tatttccatc tcaatcagtt tttaggatta aactttaatg
       61 aatgggaaaa gtactaaata aaatttacaa tatcctattt ttaggataaa aagacatctc
      121 tatggggttt tcttcatcag tgagaatcct gaaaagcacc tgatctttca gaagatcaga
      181 gacttttacc ggaatgccgt caaccagcac ccgggcgttt ataaacctta tcccatggct
      241 gtaatcaaca taccaccaga tatgaccgct gtacacgggc tggatggggc tgccatcggg
      301 gcggtgccag ccgtagatga caaccctgtc gggccgctct gcaatacgtg acgaaataat
      361 aacatccttc ttgatccctg caacgagctg accgtgcctc cagccggcat cagccagttg
      421 cctctcaatc tgctcattgt gctcttcaaa ctttgttacc agttcattcg cattgccgac
      481 cggcctgtaa aaaaacggct caggcttcac ctcagccttc gaccatacat ggtcactaag
      541 cttagccgtc agcaatgaag ccccaaacag gtcagcaagc cgctgtgctg ttcccggagt
      601 aatgggaatc cggcaaaaat catcatcact gccaattgcc aggtaatcgg gcataacctc
      661 atattcaaga tcatgaggcg tcccattcag gtcattgaac acccctttca gggtgactgt
      721 ctctctcagg aatgcaggga tatttccgga agcagcagca atgtaaatct cctcctcccg
      781 atctttcagc ggcagctcac tgattcgctt catgaactcg gaccctggtt cagctgccgg
      841 gttgcgttcc ggaatattca gcctcctcac gggaacaata attgtgtcag aaataagacc
      901 gctgtaagcc cttccgccaa aataacggta gcctttctgc tcattacgcg ttccgctcag
      961 aatgctgtgg atgaggccgt tatactccac ctgctgagta tgaaagatct tccctccggg
     1021 gtttggcttg aagatgaact caatcctcct gtcacgcgac gaataccaga gcagtgagtc
     1081 ccgttcacgc ctcttcagac tgaacgatgc ggagaggtaa tccttcattt tccagctccg
     1141 gtaccaagtg cctccttcag gcgaaaccag gggtttgccg ttgtagataa ccacactgtc
     1201 gttataggcc tgtgtgcaga gatacctgct tttccccgac cggagccagc tgactatctt
     1261 cggcccgtaa cgctcaccct cccatccgta attgctgtcg aggaaggcaa tccgcacaac
     1321 acagcccggg atctgttcat gggcatcgat atagttgaat atgaaccgcc ctccgccgct
     1381 gtggccgttc agaaccagct caggattcca gggtgcgaac agagaagtga tttcatcaac
     1441 catttttttt acgatctccg tgtggcccgg ggtattggcg ctccaggccg gccagctcct
     1501 ttgttcattc tcaagatagg ccaccaccac ggtcctgtca gttatcaccc ttctgaggaa
     1561 cctcgtctgt gcgcctatgt gctggatgtc atagtgccag tcatcgtcct ccttcagcaa
     1621 cttgccgaag gtctgttcaa tggtgttacc gtttggaagt gcataaagca caagcagcac
     1681 acggtcatcg gtgccaaatc ccgtcatggg ggcgttgatg agtactctgg tgccagcaac
     1741 tgaattttca ataactgtct gctgttcatc aaattgccct gacgatttga atccgggcag
     1801 ggactgcgag gcacccgcag cagtgatggt tgccaggatt actgtcagaa ggaaagcttt
     1861 catcttttgc agagaccgtt gatgtactcc tcgagcatcg tgtagcctgg ccctgccatg
     1921 ttattccggt cttcaggatc ggccggattc agtccctttt taagttccca tgagtcagcc
     1981 atgccgtcac cgtcactgtc ggccggcaca tcataagtct tcagcaacgg ccagccgccg
     2041 gcgctgttct gtgtgtctat tatccctgtt cccgcaccgt atgaatcgcc ataggcgcag
     2101 tagccggcca gggtctcatt aattatccgg tgatcgaccg ggtccctgtg aggcagggtg
     2161 gctccggcat ttctcagaac agacctgtaa gcacgcctgg cggaagtggt tctcaccgct
     2221 gcaacaacaa acggttcacc tgccctgatg acagggttgc ttgcattatc accctgaaca
     2281 cctccgctcc agttgtcaga cgaaaccttg ctgctgcctt cgatgcggtt tccgctgaca
     2341 tgccatcttc cgaccgtgtc ccatggttca acaatacggc tgatcacgtc tttttttgtt
     2401 gccggtccgg gcttgtagta gttattgacc atattctgct gccccatctc accgccgtac
     2461 gagctgttga atccccagtt gaagatgaca ttgttcctga aatcgaccag ctccgtctca
     2521 cgtgtatccg ggtgatatct tgctccgcag aatcgtggca gccggctggt atggctggcc
     2581 agaaggttat ggtggaatga ggcccccttg ccgccccata tcccgccgta accgtgctcg
     2641 cccttcggat ggccggcatc atacaggctc tcagagatga tgcaccattg aagagtaaag
     2701 ttctcgttgt catagaatga agcagcctcg tcaacagacc agctcatgga gcagtgatct
     2761 atgattatgt tcctgtgcct gatgcatgag atggcgtcct tgggttcttc acccgcgatt
     2821 tcattgccac aacggaatct gacatgcctt ataataacat tatcagcctc tatagaaact
     2881 gtctccccct tgatgcatat tccgtcaccg ggtgcagtct gtcccgcgat ggtgacatcg
     2941 cccttcgaga tgttcagcct ccgcttaaga tcaatggtgc cggatactgc gaacacaatt
     3001 gttttcggcg acggcttgtt aagcgcccat ctcagagagc cttcaggagg tttaaccacc
     3061 gaatcggcaa ggtttgtaac ggcatagacc gctccgcccc ttccgccggt ggtataacgg
     3121 ccgaatccct cagcgcccgg aaatgccggc aactgagcat tcaccagaat gccggctgca
     3181 agaaaaaaca gtatggcaat aatccttctc atcttaacgt ccttgctctg gctgtcccgt
     3241 cgggtgtaac tgtctcaacc gttatacggg ctgagctgct gccgcgaacc tcaacaagac
     3301 cgtctgcatc ttcagcaacc tgcaggcccg tgacatcagc atccgagctg ttgagaaaga
     3361 agataagcgg tttcttctct gtcaccagcc gcatgttcct gatctttatt cccctggcat
     3421 caatgcaagc catcccttcc actgcagtca tgtcgatgtt ttccatagtt atattctcaa
     3481 ggttgagctc aggcaatccc tgaagatata tcgcctggtc agcacccctg caagttatat
     3541 ctttcatgga aatgttcctg aactgagggg tttcttcagt gaccggaggt atatcaccaa
     3601 ccgtagtctc tatattcttc ccttcggcaa gcatctccga gaccgagagg cctccgtagt
     3661 acaggttgaa cgaaatcgcc tgcgagggga tgtctgtcat caggatgtca ttgaaccaga
     3721 tgttttctac cacccctccc ctgccgcggt tgctcttgaa ccgtatgccc acgtcagtgc
     3781 ccatgaaggt gcaaccggca acggaaacat tccttacgcc gcctgacatc tcgctcccta
     3841 ttgtcactcc tccgtgaccg tgatagacaa tgcagttcct tatcacgagg ttctcagtgg
     3901 gtatgccccg ctttcttccg tcctcattct tgcccgactt gatgcagatg gcatcatctc
     3961 ccacatcgaa attacagttg tacaggagag tgttgcggca tgactcaata tctatcccgt
     4021 ctccgttctg ggagtaccag ggatttctga cagtaatatt ccttacagta agatcctgac
     4081 acataagcgg atgaatgcac catgccggcg agttctgaaa cacaggtccg tcaatcaaaa
     4141 tctttttgca gctgatcagg cttatcatga ccgggcgcag aaaatccctc atccattcgt
     4201 atgcctcaat gcctccctcc atcggtaacg ggacgttcat ctcgctctgc tcgatggccc
     4261 tgccgt
//
LOCUS       DAOPXU010000169         4240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_27181_length_4240_cov_13.194982, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000169 DAOPXU010000000
VERSION     DAOPXU010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27181_length_4240_cov_13.194982"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..1373
                     /locus_tag="PKK03_11815"
     CDS             360..1373
                     /locus_tag="PKK03_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNX85127.1"
                     /translation="MSRENITIKDIARELGISPSTVSRALKDHPDISQATRDAVNELA
                     ERWNYRPNPIALSLKSGSSKTIGVIIPDVVHYFFSTVISGIEDVVYRRDYNMILCQSN
                     EMWEHEVKNIKTLLSSRVDGILASVAKTTTDFRHYRSITEKDIPLVFFDRAAEEIDTD
                     SVVIDDETGSYKAVIHLLRTGKKRIVHLAGPPRLAIARNRLNGYLKAMKEYRLTPQDE
                     DIVKCDDIQSAERIIPALLTRTPRPDAFFAVNDLTAAQTLMIVKRHGLRIPEDISVVG
                     FTNSQIATLTDPGLTSVDQKGFEMGQLAARLLIDRIENRRGPVQKKIITSELVIRGSS
                     STR"
     gene            complement(1380..2219)
                     /locus_tag="PKK03_11820"
     CDS             complement(1380..2219)
                     /locus_tag="PKK03_11820"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX85128.1"
                     /translation="MKLSLFNISNFRVDGGAMFGVIPKALWSRQMQSDELNTIELSLT
                     SLVIEAEKRVILVDAGWGDKQDEKFFRHAYLHGGSGLAGGLAECGYRPEDITDVIITH
                     LHADHCGGCFVNAPGGGTVPFFPNAAWHISRAQWEWANESNLREEDAFLPENIRPFGE
                     GGRLNIVEEPGELCPGVSLRICYGHTPGMLIPVIYYRGRTWVYTGDLIPTVAHIPLLW
                     NMAYDLLPLVTIAEKQEILQEALVNDWMLIFQHDPACECCTLTETPKGIRVGRKGRLA
                     DFT"
     gene            2296..3105
                     /gene="zupT"
                     /locus_tag="PKK03_11825"
     CDS             2296..3105
                     /gene="zupT"
                     /locus_tag="PKK03_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HNX85129.1"
                     /translation="MNPDFNTVLFAFLLTLFAGLSTGVGSTIAFFAKKTNTRFLTLSL
                     GFSAGVMIYVAFVEIFVKGREVLTEAMGMRQGWWAAVIAFFAGIALIGIIDKLIPSQE
                     NPHEFKKIEGGEEEQRSGRLLRMGAMTAIAIGIHNFPEGLATFTAALSDPQIGIAIAV
                     AVAIHNIPEGIAVSVPVYYATGSKRKAFRLSFLSGIAEPVGALAGYLLLLPIMTDTLM
                     GVLFSFVAGIMVFISLDELLPAAEKYGEHHLSIYGLVSGMAVMALSLLLFK"
     gene            3175..3642
                     /locus_tag="PKK03_11830"
     CDS             3175..3642
                     /locus_tag="PKK03_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85130.1"
                     /translation="MKRTLTVILVLAALTFGVQAQSYKSAIGLRGGDPSGITFKTFLN
                     STNALELIAGTGYWGHNFAVTGFYQWQNPTDWTPNLDWFIGPGAHVGFWNDYWKNEYS
                     TGILLGVDGIIGLEYTFDDIPLNLGFGVGPSIQLTSGPGFFYWNGGFSVRYVF"
     gene            3760..3831
                     /locus_tag="PKK03_11835"
     tRNA            3760..3831
                     /locus_tag="PKK03_11835"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3793..3795,aa:Glu,seq:ttc)
     gene            3855..4109
                     /gene="rpsT"
                     /locus_tag="PKK03_11840"
     CDS             3855..4109
                     /gene="rpsT"
                     /locus_tag="PKK03_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006254600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNX85131.1"
                     /translation="MANHKSALKAVRQAETRKEDNKYYARTMRNSLKKMRSTTEKTEA
                     EKMLPELNAMLDKLAKKNVIHKNKASNLKSSITKHAQSLK"
BASE COUNT         1115 a         1078 c          994 g         1053 t
ORIGIN      
        1 actccgggta acgtctgtct gtcggctgcg tcggtaactg tacctgacac tcttacctgt
       61 tgcccgaaga caacaagact catcattatg ccgaaggtca gcatgaagag ccgttttcca
      121 gttagtttct ccataaatag atcagtttaa ttacttttca gaatttgagt tattgctaaa
      181 ttaatttgtc caggtttggg acgataaaag tcgatttttt ttcagagtta accaactctt
      241 tttcagcttt tttccgcaat cgttaccgca aacgtttgaa ttgaggacga ggcatatttt
      301 catgatcccc cggatagcaa attttttatt atatttaaca tctaatagct ccgtctcata
      361 tgagcaggga aaacatcaca ataaaggaca tcgcacgcga actcggaatt tctccctcaa
      421 ctgtatcaag ggccctgaaa gatcatcctg acatcagcca ggctacccgc gatgccgtca
      481 atgaacttgc cgaacgctgg aactacagac ccaatcccat cgccctgagc ctgaagagcg
      541 gatcctcaaa aactatcggg gtgatcatcc ctgatgtcgt acattatttc ttctcaacag
      601 tcatcagcgg tatcgaggat gtggtctacc ggcgtgatta caacatgatt ctctgccaga
      661 gcaatgagat gtgggagcat gaggttaaga acattaagac tcttctttcc agccgcgttg
      721 acggcatcct tgcatctgtt gccaagacca ctacagactt caggcattac cggagcataa
      781 ctgaaaagga tataccgctg gttttctttg acagggccgc tgaagagatt gacaccgaca
      841 gtgttgtcat tgatgacgag accggatcat acaaggctgt gatccatctg ctccgcacgg
      901 gaaagaagcg catcgttcac cttgcaggcc cgccccggct ggcaatagcc cgtaacaggc
      961 tcaacggcta tctgaaggca atgaaggagt accgcctcac tcctcaggat gaagatatcg
     1021 tcaaatgcga tgatatccaa agcgccgaga ggattatccc cgcattactg acacgcaccc
     1081 cgagacctga tgcatttttt gccgtaaatg acctgacggc agcacaaact ctcatgatag
     1141 tcaaaaggca cggactgagg atccctgaag atatttccgt cgttggattc accaacagcc
     1201 agattgccac cctgacagat ccgggcctga catccgttga ccagaagggg tttgagatgg
     1261 gacaattggc tgcaaggctg ctgattgacc gcattgagaa ccgccgcggt cctgttcaga
     1321 aaaagatcat cacctcagaa ctggtgatac ggggttcttc atcaaccagg taatgccgtt
     1381 caggtaaaat cggcaagcct cccctttctt cccaccctga tccctttcgg tgtttccgta
     1441 agagtacagc attcacaggc cggatcatgc tggaatatca gcatccagtc attcaccagg
     1501 gcctcctgaa gtatctcctg tttttcggca attgttacga ggggaaggag atcataagcc
     1561 atgttccaaa gtaacgggat atgcgcaacc gtggggatga ggtcaccggt gtatacccat
     1621 gtcctgccgc gatagtatat tacaggaatg agcattcccg gagtatggcc gtagcatatc
     1681 ctcagtgaca ctcccgggca cagttcgccg ggctcctcaa caatgttcag ccttccgcct
     1741 tcgccgaatg gcctgatgtt ctcgggaaga aaagcatcct cctcacggag gtttgattca
     1801 tttgcccatt cccactgggc acggcttatg tgccaggcgg cattggggaa aaaaggtacg
     1861 gtgccgcctc ccggtgcgtt tacaaaacaa cctccgcaat ggtcggcatg cagatgggtg
     1921 attatcacat ccgtgatatc ctcaggtctg taaccgcatt ccgccagacc gccggcaagc
     1981 cctgatccgc catgaagata ggcgtgacgg aagaactttt catcctgctt gtcaccccaa
     2041 cccgcatcaa cgagaatgac cctcttttcg gcctcaatga caagcgaggt gagcgacaac
     2101 tcaatggtat tcaactcgtc tgactgcatc tgccggctcc agagggcctt cgggatcacc
     2161 ccgaacatcg ccccgccatc aacccggaaa tttgatatgt tgaaaagaga taatttcatt
     2221 ttatggccct ttaacggatg caagataacc aattttggta tttttgactg atgatatagc
     2281 aatgctgaaa cagcaatgaa ccccgacttc aacacagtac tttttgcgtt cctgcttact
     2341 ctgtttgccg ggctgtcaac cggtgtgggc agtacgattg cattcttcgc aaagaagacc
     2401 aacaccaggt ttctcactct tagcctgggc ttctcagccg gtgtgatgat atatgtggct
     2461 ttcgttgaga tctttgtcaa gggccgcgaa gtgctgaccg aagcaatggg catgcgccag
     2521 ggatggtggg cagccgtaat cgccttcttt gccggcatag ccctcatagg catcattgac
     2581 aaactcatcc cgtcacagga gaaccctcat gagttcaaaa agatcgaagg aggcgaggag
     2641 gaacagagaa gtggcaggct gcttcgcatg ggagcaatga ctgccatcgc cattgggata
     2701 cacaacttcc ccgaagggct ggcaacattc actgcagcac tttctgaccc tcagataggt
     2761 atagctattg ccgtagctgt ggcaatacac aacatccctg aaggcatagc cgtctcagtg
     2821 ccggtatatt atgccacagg cagcaagaga aaagcattca ggctctcctt tctgagcggc
     2881 attgccgaac cggtaggagc cctcgcaggt tatctgctgc tcctcccgat catgacggac
     2941 acgcttatgg gcgtgctctt ttcattcgtt gccggaatca tggtatttat ctcactcgac
     3001 gaattgctgc cggcagcaga gaaatacggc gagcaccacc tttctatata tggccttgtc
     3061 tcaggtatgg ccgtgatggc gctcagtctg ttgctcttta aataaacaaa attttcccga
     3121 atctgttaga taaatatata tttgcaagtt caatacaaac taaacaaaat tatgatgaaa
     3181 agaacactga ctgtcatact ggtactggca gcactgactt tcggagtgca ggctcagagt
     3241 tacaaatcag ccattggcct caggggaggt gatccttcag gtattacctt caagacattc
     3301 ctcaacagca ccaacgcact tgaactgatt gcaggaactg gttactgggg tcataacttt
     3361 gcagtaaccg gattctacca gtggcagaat cctactgact ggactcccaa tctcgactgg
     3421 ttcatcggtc ccggagcaca tgtcggattc tggaatgatt actggaagaa tgaatacagc
     3481 accggtatcc ttctcggtgt tgacgggata ataggactgg agtacacatt tgatgacata
     3541 ccgctgaacc tcggcttcgg agtaggcccc tccattcagc tcaccagcgg accgggcttc
     3601 ttctactgga acggcggatt ctcagtaagg tacgtcttct gagaaggccc tgcattgtca
     3661 tttcggagcg gatcagaata agaagcattt tttctgcata tttttttgca gattaaaaaa
     3721 aagatgcgat ctttgcatcc gcttttttaa gttctttaag gtccattcgt ctagtggtta
     3781 ggacgtcagg ttttcatcct ggtaacaggg gttcgattcc cctatggact actaaattac
     3841 aataaacaac aaaaatggct aaccataagt cagcactcaa agccgtaaga caggccgaga
     3901 caaggaagga agacaacaag tactacgccc gtacgatgcg taactcgctc aagaagatgc
     3961 gcagtaccac cgagaagaca gaggcagaaa agatgcttcc cgaacttaat gccatgcttg
     4021 acaagctggc aaagaaaaac gtcatccata agaacaaggc ttccaacctc aagtcttcaa
     4081 taacgaaaca cgctcagagc ctgaaatagg gttctactcc aaatatttca cagccgtcat
     4141 ttccttgtga cggctgtttt ttttatattg cagccgggat aaattgtttt gctgcatgtc
     4201 aatgaagatc acagactggg ctgtcgagga caggccccgt
//
LOCUS       DAOPXU010000170         4153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_27976_length_4153_cov_12.476818, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000170 DAOPXU010000000
VERSION     DAOPXU010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27976_length_4153_cov_12.476818"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="PKK03_11845"
     CDS             complement(<1..175)
                     /locus_tag="PKK03_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNX85132.1"
                     /translation="MKKLKFEEPFLTESGEVLTRLEIAYNTYGALNGEGNNVIWVCHA
                     LTANSDVESWWPGM"
     gene            complement(172..1476)
                     /locus_tag="PKK03_11850"
     CDS             complement(172..1476)
                     /locus_tag="PKK03_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNX85133.1"
                     /translation="MENSKLRFETLQLHAGHTPDSDTLSRAVPLYQTTSYVFRNAKHA
                     ADLFGLAEPGNIYTRIMNPTTDVFEKRVAALEGGAAALAVSSGHAAQFIALTTFLRAG
                     DNFVSSPFLYGGTYNQFNVTLPSFGINVRFSADLNLASFERLIDDNTKAIYLETIGNP
                     GFDIPDFEAFAGLASRYAIPLVVDNTFGACGYLCAPIKYGANIVVESATKWIGGHGTS
                     IGGVIVDAATFEWRNGKFPSLTEPAKGYHELVYTDVFSPDTEAGNIGFIIKARVEGLR
                     DLGPAISPFNSFLLLQGLETLSLRMERHIQNTIQLARWLKEQSFIERVTYPGLEENPY
                     HEVAKKYLPNGAGGVLSFVLKTDLGKAVKLVEHLKIVSHLANVGDAKTLIIQPSATTH
                     SQLSPEEQLAAGVEPGLFRVSVGLEHIDDIIGDFSQAFRKII"
     gene            1818..2243
                     /locus_tag="PKK03_11855"
     CDS             1818..2243
                     /locus_tag="PKK03_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85134.1"
                     /translation="MKKLLSILTVLLVVTAMLHFSVARHYCHGEMVASKVSLSGALAT
                     CGMEDNEGDCRHGHNEDLFDSHCCDDVVTSYFLDNTYTPATKAKSGLDHAKLQVPVMP
                     VEKPARVAFTEARSLSDISPPGGLMTSSVDLPQIRVFRI"
     gene            2350..2724
                     /locus_tag="PKK03_11860"
     CDS             2350..2724
                     /locus_tag="PKK03_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNX85135.1"
                     /translation="MKSIRLFISVLALSLFTIPAFTQAHDHAKNTVAGDTVKTETFKV
                     WGACGMCKTRIEKTVKTEGATAASWDSKTQLLTVTYNPAKTGKEALSKKLAAAGHDTE
                     KFKAPDEVYNSLPGCCHYERQK"
     gene            2839..>4153
                     /locus_tag="PKK03_11865"
     CDS             2839..>4153
                     /locus_tag="PKK03_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX85136.1"
                     /translation="MFNRLVKYFLENRLITFILLIAFVVMGLVTMPFSLKSGLLPRDT
                     VPVDAIPDIGENQQIVATEWMGRSPQDIQDQVTYPLTTALLGIPGVQTIRSTSMFGMS
                     FIYIIFKDNVEFYWSRSRILEKLNSLPPGTLPEGVQPTLGPDATALGQIFWYTLEGRN
                     PETGEPAGGWDPSELKTIQDFYVKYSLSAAEGVSEVASAGGFLKEYQVDLDPEALKAY
                     NVSIMDVMNGVKRSNLDIGAETIELNNVEYIIRGLGYVKSLNDLELSVITVRENVPVR
                     IRDVAVVSFGPAPRRGGLDKAGSEAVGAVVVARYGSNPMEVINNVKEKIKEIEAGLPQ
                     KSLPDGTVSKVTIVPFYDRTGLIKETIGTLESALSHEILISIIVIIVLVMNLRASLIV
                     ASLLPIGVLMTFILMRFLGIDANIVALSGIAIAIGVMVDIGIVDVE"
BASE COUNT         1077 a         1006 c         1018 g         1052 t
ORIGIN      
        1 ccattcccgg ccaccacgat tccacatcag agttcgcggt aagggcatga cagacccaga
       61 taacattatt tccctcccca ttcagcgcac cataggtatt gtaggctatc tccaggcgtg
      121 taagcacctc tcctgattct gtcagaaaag gctcctcgaa tttcagtttc ttcatattat
      181 cttcctgaat gcctgtgaaa agtcaccgat gatgtcatct atatgctcca gtccaaccga
      241 caccctgaag aggccaggct ccaccccggc agcaagctgc tcttcgggtg aaagctgtga
      301 gtgggtggtt gctgacggtt gtattatgag tgtcttggca tcacctacgt tggcaagatg
      361 actcactatt ttcaggtgtt ctacaagttt tactgctttc ccaagatctg tcttcaaaac
      421 aaaactcagc accccgccgg caccgttggg aaggtatttt tttgccactt catgatacgg
      481 gttttcctcc aggcccgggt atgtgaccct ctctatgaag ctctgttctt tcagccaccg
      541 ggccagttga attgtgttct gtatatgcct ctccatgcgc agtgagagcg tctcgagacc
      601 ctgcaggagc aggaaggagt tgaacgggct gatagcagga ccaaggtccc tgaggccttc
      661 aaccctcgct ttgatgataa acccgatatt gccggcttca gtgtcaggcg aaaatacatc
      721 agtgtagaca agctcgtggt accctttcgc cggctccgtc aatgagggga atttcccgtt
      781 tcgccactca aaggtggcag catcaacaat gactcccccg atgctcgtac catgtccccc
      841 tatccatttt gtggcagact ctacaacaat gtttgcaccg tactttatgg gtgcacacag
      901 atacccgcag gctccgaaag tattatcaac caccaggggt atcgcgtaac gagacgccag
      961 tccggcgaat gcctcaaaat caggaatgtc aaagccgggg ttgccgattg tctcaagata
     1021 aatggccttt gtgttgtcat cgattagtct ctcaaaggag gcaagattga ggtcagcaga
     1081 aaaccttaca tttatgccga atgaaggaag tgttacgttg aactggttgt aagtgccccc
     1141 gtacaggaac ggtgaactga caaagttgtc tcctgccctc aggaatgtgg tcagggcaat
     1201 gaactgtgca gcgtggcccg aggacaccgc cagtgccgca gcgcctccct ccagcgctgc
     1261 cacacgcttt tcaaagacat cagttgtcgg attcattatc ctcgtataga tattgccagg
     1321 ctcggcaagg ccgaagagat cggcagcatg tttcgcattt ctgaatacat aggatgttgt
     1381 ctgataaagg ggaactgccc ttgacagggt gtcactatcg ggtgtgtgac ctgcatgcag
     1441 ctgcagagtc tcaaatctga gtttactgtt ttccatgata acaaaattga tatgaatact
     1501 aaggcggata ccgcaacagg atcaatcctt ccatcattac gtggcggtaa aatctaaaac
     1561 gacaatatca ggggaaggag attaccggca catgcacata ggcatgagcg gcatgatgaa
     1621 attgttatgg ttgagatgaa tgtaattcat gttctccttt tactccggca gaggtctgcc
     1681 ggagctgcaa aaataatcaa atttgttccc ggtatgcgga aaccgggaaa aaatctccca
     1741 aacaacacgg tttccgaacg gaatataaaa agcattaact ttgtaaacca ttttaacgga
     1801 actttgttga ttattttgtg aaaaagttac tctccatact gactgtactt ctggtggtga
     1861 cagcaatgct gcacttctcg gttgcgcgtc attattgcca cggagagatg gttgcttcaa
     1921 aggtttcact ttcgggggcc ctggctacct gcggaatgga agataatgaa ggtgactgca
     1981 ggcatggaca taatgaggac ctttttgact cccattgctg tgatgatgtc gtgacatcat
     2041 attttcttga caatacttac actcctgcca caaaagccaa gtccggtctt gaccatgcga
     2101 aattacaggt tccggtcatg ccagttgaga aacctgcaag ggttgctttt accgaagcaa
     2161 ggtcattgtc tgatatcagt cctcccggag gattgatgac atcttcggtt gatctcccgc
     2221 aaattcgggt attccgtatc tgatttcatt ttaccgtcat tgccgttgca ttggggcttt
     2281 gttcccggtc tgcatcggct tctgtttatt ctactgctaa aatcattcaa actttaaaaa
     2341 tttgaaatca tgaaatcaat acgactcttt ataagtgtac tggcattgtc actgtttaca
     2401 ataccagcat ttacccaggc gcatgatcac gccaaaaaca ccgttgccgg agatacggta
     2461 aaaacagaaa ctttcaaggt atggggcgca tgcggaatgt gcaagaccag gatagaaaaa
     2521 actgttaaaa ctgagggtgc tacagcagcc tcatgggact ctaaaaccca actcctgacg
     2581 gttacttata atcctgcaaa aaccggcaag gaagctttga gcaagaagct tgctgctgcc
     2641 ggacacgata cggagaaatt caaggctcct gacgaggtct ataacagtct tccgggctgc
     2701 tgccattacg agaggcagaa gtaaatttcc agtattcaca ttttgaacct ggctgctgaa
     2761 gccacagtga ggccatgaca tcaggtcaga gggcagccct gtggccggca caggaattaa
     2821 agttttaacc acaatgccat gtttaacaga ctagtcaaat acttccttga aaaccggctt
     2881 ataaccttca tcctcctcat agctttcgtg gtgatggggt tggtgacaat gccgttcagc
     2941 ctcaagtcag gccttttgcc acgcgatacg gttcctgttg atgcaatccc ggacatcggt
     3001 gaaaaccagc agattgttgc caccgaatgg atgggccggt ctccccagga tatccaggac
     3061 caggtcactt atccgcttac aaccgccctg ctgggtattc cgggcgttca gacaataaga
     3121 agcacctcta tgttcggcat gtcttttatc tatataattt tcaaggataa tgtcgaattc
     3181 tactggagcc ggtcaaggat actggagaag ctgaactcac ttcctccagg cacattgccg
     3241 gaaggggtgc agccgaccct ggggcccgat gccacagcac tcgggcagat tttctggtat
     3301 acacttgaag gacggaatcc cgaaaccggg gaaccggcag gaggctggga cccgtcagaa
     3361 ctaaagacaa tccaggactt ttacgtgaaa tacagcctct cggctgcaga gggggtctcc
     3421 gaggtagcat ctgcaggcgg attcctgaag gaataccagg ttgacctgga tcccgaagcg
     3481 ctcaaggctt acaatgtatc cattatggat gtgatgaatg gagtgaagcg aagcaacctg
     3541 gacataggag ctgaaacaat tgaacttaac aacgtcgagt acataatacg cggcctcggt
     3601 tatgtcaaga gcctgaatga cctcgaactg tctgtaatta cagtccggga aaatgttccg
     3661 gtgcgaatca gggacgtagc cgtcgtcagc ttcggccctg caccgcgaag aggcggactt
     3721 gacaaagccg gctcagaggc tgtgggtgca gttgttgttg ccaggtacgg ctcaaacccg
     3781 atggaggtga ttaacaatgt aaaagaaaaa atcaaggaga ttgaagcagg cctgccgcag
     3841 aaatccctgc ctgacggtac ggtctcgaag gtcacaattg ttccctttta tgaccggaca
     3901 ggcctgataa aggaaacgat aggcaccctt gagtccgcgc tgtcacacga aattctcata
     3961 agcataattg taataattgt gcttgtcatg aaccttaggg catcacttat tgtggcaagc
     4021 ctcctgccaa ttggcgtcct gatgactttc atcctgatgc gttttctggg tattgatgcc
     4081 aatattgtcg ctttatcagg tattgccatc gccatcggag taatggtaga catcgggatc
     4141 gttgatgttg aga
//
LOCUS       DAOPXU010000171         4142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_28076_length_4142_cov_13.335699, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000171 DAOPXU010000000
VERSION     DAOPXU010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28076_length_4142_cov_13.335699"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PKK03_11870"
     CDS             complement(<1..506)
                     /locus_tag="PKK03_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HNX85137.1"
                     /translation="MDNFKMRLARLREEHEALIKRRNKKIEPGNGIFDRYAYPVLTGA
                     HAPLEWRYDLNPATNPHLMERFGINAAFNAGAMKFNGKYILAVRVEGYDRKSFFAIAE
                     SQNGVDNFRFRDYPVTMPETEDPDINVYDMRLTRHEDGWIYGVFCSERKDPKAAPGDL
                     SSAVAAAG"
     gene            complement(519..1649)
                     /locus_tag="PKK03_11875"
     CDS             complement(519..1649)
                     /locus_tag="PKK03_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNX85138.1"
                     /translation="MYKTFIITAVMLTMTACSSGDTKRTGPADRSATPETVNMLAGLK
                     QAAGQGIMFGHQDDLSYGIGWVYPGGESDVKRVTGDYPAVYGMDLGDIEHRSPVNLDS
                     VPFDDMKVFVQQIWERGGVITFSWHVDNLLTGGTAWDVSSDRAVASVLPGGEKHEEFK
                     DWLDNLAGFFASLRDEKGVAVPVIFRPWHEHTGSWFWWGQNLCTTEEFVALWRFTFDY
                     LCREKNLHNLLFTYSSGGDIDNSEQYLERYPGDDYVDLIGFDYYQMPDAGNSSFRKNV
                     SRVLGIVTEAAASHGKLAALTEAGYEGIPDSAWWTTTFWPAMENHRISYALVWRNAHN
                     RPGHFYAPYPGQVSGQDFISFYGFRETLFQSEVTALSIYKQE"
     gene            complement(1657..2961)
                     /locus_tag="PKK03_11880"
     CDS             complement(1657..2961)
                     /locus_tag="PKK03_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-mannosidase"
                     /protein_id="HNX85139.1"
                     /translation="MKGKVFAGLLLMIALAGCGSSQKYDYVTVDDGIFRLAGEPYYFI
                     GANYWYGAILGTEGQYGDRGRLLRELDHMKSIGITNLRIMVGPEGPDNEPFRVTPALQ
                     AAPGEYSGEMLDGLDFLLAEMGKRGMHAILFLNNAWDWSGGFSQYLSWNGYGPIPNPN
                     VPPNTWPQFMAFSGQFNTCEPCIRQFEEFIRFILGRTNTCTGVKYTDDPAIMTWEIAN
                     EPRAFSDENIPAFGEMIARIAALLQELDENHLVTTGTEGRHGCEQKMELFESIHADPN
                     IDYLVMHIWPKNWGWLDIADIAGTLDSSVVKTNRYIDDHLEVAVRLHKPIVLEEFGLP
                     RDLHGFSPDESTVARDAYYANAFGQVLEHAGRLGPLAGCNIWAYAGEGRAAEGRIHWL
                     PGDDYMGDPPQEEQGLNSVFDTDSTTGIISDFSLRLSSLTKN"
     gene            3258..4139
                     /locus_tag="PKK03_11885"
     CDS             3258..4139
                     /locus_tag="PKK03_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNX85140.1"
                     /translation="MHDNILREITPLTQSDCFTYFYRAKSEFDFPLHYHDEIELNFIR
                     NAKGAKRIIGDHVEEIGNLELALIGPGLPHGWFGTGFAPDQVEEVTIQFHKDFFDEKF
                     LQKNQLNFIRNMLRRSLRGIIFSPETTARLEPRIVSLGKKQGFDSVLELMSILHDLSI
                     SRDYRVCSDSGFSDTEVFNYNSRRIEKVMEFIHSSFDKQITLGEAARIASMTESAFSR
                     FFKLRTGMTFIDCVTEVRLGHASRMLISTTKTIAEIAYSCGFNNISNFNRIFRRKKSC
                     TPRELRESYDEGMRVFI"
BASE COUNT         1011 a         1119 c         1014 g          998 t
ORIGIN      
        1 atgccggcag cggcgactgc tgaagagaga tcacccggtg cagctttcgg atccttccgc
       61 tcactgcaga agacaccgta gatccatccg tcctcatgcc gtgtaagcct catgtcataa
      121 acgttgatgt ccgggtcctc ggtctccggc atggtgaccg gataatcacg gaagcggaaa
      181 ttgtcaacgc cgttctgact ttcggcaatg gcgaagaatg atttcctgtc gtatccttcg
      241 acccttactg caaggatata tttgccattg aacttcattg ccccggcgtt gaaggcggcg
      301 ttgatgccga atcgctccat caggtgggga ttggtggcag gattgaggtc atatctccat
      361 tccagcggtg catgggcgcc ggtaagcacg ggataagcat acctgtcaaa gattccgttg
      421 ccgggttcaa tctttttgtt acgacgtttg ataagcgcct catgctcttc cctgagtctt
      481 gcaaggcgca ttttgaaatt atccatctgg ttaaaaaatt attcctgttt atatatgctg
      541 agggccgtca cttcactttg aaagagagtt tccctgaaac cataaaaact gataaaatcc
      601 tgcccgctca cctgacccgg gtacggtgca tagaaatgtc ccggccggtt atgtgcatta
      661 cgccatacca gggcgtatga tatacggtga ttctccatcg ccggccagaa cgtggtggtc
      721 caccacgctg aatcgggtat gccctcatat cccgcttccg tcagtgcagc cagcttacca
      781 tgagacgcgg cagcttcagt gactatgccg agcaccctgc tcacattctt cctgaatgaa
      841 ctattaccgg catcaggcat ctgataatag tcgaaaccga taagatcaac gtagtcatct
      901 cccgggtaac gctcaagata ctgctcgctg ttgtcgatat cacccccgct ggaatacgtg
      961 aagagcaggt tatgaaggtt cttctcccgg cacaggtaat caaaggtgaa tctccacaat
     1021 gcaacaaact cttcggttgt gcaaagattc tgtccccacc agaaccagct tcccgtatgc
     1081 tcatgccagg gccggaagat taccgggaca gccaccccct tttcatccct gagagaggca
     1141 aaaaatccgg caaggttatc aagccagtcc ttgaactcct cgtgtttttc tcctccgggc
     1201 agcaccgaag cgactgccct gtcagaagac acatcccatg ccgttccgcc ggtaaggagg
     1261 ttatcgacat gccagctgaa agtgatgacc ccgccgcgct cccatatctg ttgcacaaaa
     1321 actttcatat catcaaatgg aacagaatcc aggttcactg gtgaccggtg ctcgatgtca
     1381 ccgaggtcca tgccataaac agccggatag tctccggtga ctcttttcac atccgattca
     1441 cctccggggt agacccaccc gattccgtac gagaggtcat cctggtgtcc gaacattata
     1501 ccctgcccgg cagcctgctt cagccctgcc agcatgttaa cagtctcagg cgtagcagac
     1561 ctgtcggcag gcccggttct tttggtgtcg cctgagctgc aggcagtcat ggtcagcatt
     1621 acggcggtga taataaaagt cttatacata aagggatcaa tttttggtga gagatgagag
     1681 gcgaaggctg aaatcggata tgatgcctgt ggtggagtcg gtgtcaaaaa cagagttaag
     1741 gccctgctcc tcctgaggcg gatcacccat gtagtcatca ccgggcagcc agtgaatgcg
     1801 tccctcagca gccctgccct ctccggcgta ggcccatatg ttgcatccag ccaggggccc
     1861 gaggcgaccg gcatgctcaa gcacctggcc gaaagcgttg gcgtaatagg catcacgcgc
     1921 aaccgtcgac tcatcaggcg aaaatccatg gagatcacgt ggcagcccga actcctcgag
     1981 cactatcggt ttgtgcagcc gtacggcaac ttcaagatgg tcatcaatat accggttggt
     2041 tttaaccacc gatgaatcca gggttccggc aatatccgca atgtcaagcc agccccagtt
     2101 cttgggccat atatgcatga ccaggtaatc aatattcgga tcagcgtgaa tgctctcgaa
     2161 gagctccatc ttctgctcgc agccatggcg gccctcagtg ccggtggtaa caagatgatt
     2221 ttcatcaagc tcctgcagca atgcagcaat gcgtgcaatc atctccccga aagccgggat
     2281 gttctcatcg gagaatgccc gcggttcgtt ggctatctcc caagtcatga ttgcagggtc
     2341 atcggtatac ttaacaccgg tgcaggtgtt tgtacgtccc aggataaaac gtatgaactc
     2401 ctcgaactgc ctgatgcacg gctcgcaggt gttaaactgc cccgagaaag ccatgaactg
     2461 cggccatgtg tttggcggca cattggggtt gggtatgggt ccgtaaccgt tccagctcag
     2521 atactgggaa aagcccccgc tccagtccca tgcgttgtta agaaagagga tggcgtgcat
     2581 cccccgtttg cccatttcgg caagcaggaa gtcgagcccg tcaagcatct ccccactgta
     2641 ttcacccggg gccgcctgca gcgcaggggt aaccctgaaa ggctcattgt cagggccttc
     2701 aggaccaacc atgatccgga ggtttgtgat gccgatactc ttcatgtgat ccagctcccg
     2761 gagcagccgc ccgcggtcac catactgtcc ctcagttcca agtatggcac cgtaccagta
     2821 gtttgctccg ataaaataat atggctctcc ggcaagcctg aagataccgt catcaactgt
     2881 cacataatca tatttctgac tgcttccgca acctgcgagg gcaatcatca gcagcagccc
     2941 tgcaaatacc tttcctttca tgcgattaca tacgatctta atcaaaatta agcaatcact
     3001 cccgctggcg ggtaatacca ttcttctaaa tagtgatact ttaatgccat tatcttcagt
     3061 gtaataacat aaaatatgca acataccgat acatttttag ccgtcaccgc ccggggaaag
     3121 atgtcatggc tgtatggtta cggtccttaa acccttattt catgcacatt acatcaaaca
     3181 ggaatcagga gccaggaata tattatatta acacttcttt tatatctttg aactgactat
     3241 actatattaa tccggccatg cacgacaata tactgagaga gattactccc cttacccagt
     3301 ctgactgttt cacctatttc tacagggcga agtcggagtt tgactttccc cttcattatc
     3361 atgatgagat agagctcaat ttcataagga atgcaaaggg tgcgaaacgg atcatcgggg
     3421 atcatgtaga ggagataggc aacctggagc tggctctcat cgggcccggg ctgccgcacg
     3481 gatggttcgg gacaggcttt gctcccgacc aggttgaaga ggtgaccata cagttccata
     3541 aggatttctt cgatgagaag ttccttcaga agaaccagct gaatttcatc cgtaacatgc
     3601 ttcgcaggtc actgcgcggc attatcttct ccccggagac gacggccagg ctggagcccc
     3661 gcatagtgag cctcggcaaa aagcagggtt ttgattcggt gcttgagctg atgagcatcc
     3721 ttcatgatct ctcaatctcg cgtgactacc gtgtatgttc tgattcagga ttcagtgata
     3781 ccgaagtgtt caattacaac agcaggcgga tagagaaggt gatggagttc attcacagtt
     3841 cgtttgacaa gcagataacc cttggcgagg cagcaaggat agccagcatg acagagtcag
     3901 ccttcagccg atttttcaag ctgcgcaccg gcatgacatt tattgactgt gttacggaag
     3961 tcagactggg tcatgcctca aggatgctga tttccaccac caagacaata gctgaaatag
     4021 cctactcatg cggcttcaac aacatctcca acttcaaccg cattttccgc aggaagaaga
     4081 gttgcacccc ccgtgagctc cgggagagct acgatgaggg gatgagggta tttatctgac
     4141 ct
//
LOCUS       DAOPXU010000172         3958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_30057_length_3958_cov_13.904432, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000172 DAOPXU010000000
VERSION     DAOPXU010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30057_length_3958_cov_13.904432"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /gene="rplR"
                     /locus_tag="PKK03_11890"
     CDS             complement(<1..303)
                     /gene="rplR"
                     /locus_tag="PKK03_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNX85141.1"
                     /translation="MALAKTEKREKIKLRIRKRIRGTAEKPRVAVFRSNKQIYAQAVD
                     DVNGVTLAAASSREKEIASVTGKKKSEVAVLVGKRLAAACREKGIETVVFDRSGYQY"
     gene            complement(324..878)
                     /gene="rplF"
                     /locus_tag="PKK03_11895"
     CDS             complement(324..878)
                     /gene="rplF"
                     /locus_tag="PKK03_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNX85142.1"
                     /translation="MSRIGKLPVNLPSGVTLSVSDDNVVSVKGPLGTLSQKVDSDIKV
                     EVENNTIILARPNDEKRLKSLHGLYRALIANMVTGVSKGYRKDLELVGVGYRAEANGQ
                     RLEMSLGYSHDIVMELPQEIKVETKTEKRSNPVITLSSIDKQLIGHVAAKIRSLRPPE
                     PYKGKGIKFVGEQLRRKAGKSAGV"
     gene            complement(894..1289)
                     /gene="rpsH"
                     /locus_tag="PKK03_11900"
     CDS             complement(894..1289)
                     /gene="rpsH"
                     /locus_tag="PKK03_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNX85143.1"
                     /translation="MTDPIADFLTRVRNAIRAGHKVVETPAAGLTKDITRILYDKGYI
                     LSYKFTDDDLQGKIKIALKYHPKTKLPAIKQIQRVSRPGLRRYAGVDELPRVLNGLGI
                     AILSTSRGVMTEKEAAKENVGGEVICYVY"
     gene            complement(1325..1594)
                     /gene="rpsN"
                     /locus_tag="PKK03_11905"
     CDS             complement(1325..1594)
                     /gene="rpsN"
                     /locus_tag="PKK03_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNX85144.1"
                     /translation="MAKESMKAREVKRAKLAKRYAAKRAALKAEGNYVALSTLPRNSN
                     PIRQRNRCKLTGRPRGYMRQFGISRITFREMASKGLIPGVRKASW"
     gene            complement(1606..2151)
                     /gene="rplE"
                     /locus_tag="PKK03_11910"
     CDS             complement(1606..2151)
                     /gene="rplE"
                     /locus_tag="PKK03_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNX85145.1"
                     /translation="MYTPALRTKYKNEITPALVKQFNYTTVMQVPRLEKIVINQGLGQ
                     AIADKKLLEMGQKEVSDIAGQKAVQTISRKDISNFKLRKAMPIGVMVTLRQDRMYEFL
                     ERLISVALPRIRDFKGVSAKLDGRGNYTLGITEQIIFPEIDLDKIAKIMGLQITFVTT
                     AKSDEEAYALLKEFGIPFKNK"
     gene            complement(2151..2474)
                     /gene="rplX"
                     /locus_tag="PKK03_11915"
     CDS             complement(2151..2474)
                     /gene="rplX"
                     /locus_tag="PKK03_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNX85146.1"
                     /translation="MQKKLHIKKGDTVAVIAGESKGQKGRVLEVDRAKNKALVEGINM
                     VSKHTKPNAKAPQGGILKKEGPIHISNLMVIDPATGKPTRVGRKLNDKNKLVRYAKKS
                     GEEIK"
     gene            complement(2493..2858)
                     /gene="rplN"
                     /locus_tag="PKK03_11920"
     CDS             complement(2493..2858)
                     /gene="rplN"
                     /locus_tag="PKK03_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNX85147.1"
                     /translation="MIQQESRLNVADNTGAKEVLCIRVLGGSKKRYASVGDKIVVSVK
                     SALPSGEAKKGSVSRAVVVRTTKEIGRADGSYIRFDDNAVVLLTNQDEVRGTRIFGPV
                     ARELRDKYMKIVSLAPEVL"
     gene            complement(2861..3118)
                     /gene="rpsQ"
                     /locus_tag="PKK03_11925"
     CDS             complement(2861..3118)
                     /gene="rpsQ"
                     /locus_tag="PKK03_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNX85148.1"
                     /translation="MEERNLRKERTGVVVSNKMDKSIVVAIHRKVKHPIYGKFVSKTK
                     KLMAHDEENSCNIGDTVRIMETRPLSKNKSWRLVEIIERAK"
     gene            complement(3129..3332)
                     /gene="rpmC"
                     /locus_tag="PKK03_11930"
     CDS             complement(3129..3332)
                     /gene="rpmC"
                     /locus_tag="PKK03_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNX85149.1"
                     /translation="MKSSEIRELSNQELLERIDNEKTALVRMKLNHAISPLENPQKIK
                     ESRKTVARLMTEMRKREINEKSK"
     gene            complement(3345..3767)
                     /gene="rplP"
                     /locus_tag="PKK03_11935"
     CDS             complement(3345..3767)
                     /gene="rplP"
                     /locus_tag="PKK03_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNX85150.1"
                     /translation="MLQPKKTKFRRSQKGKMKGNAQRGNQLAFGSFGIKTLEEAWITG
                     RQLEAARQAVTRHMKREGQIWIRVFPDKPITKKPAEVRMGKGKGAPEGFVVPVTPGRI
                     IIEAEGVPFDVAKEALRLAAAKLPVATKFVVRHDYAEE"
BASE COUNT          733 a         1050 c          938 g         1237 t
ORIGIN      
        1 atactggtat ccgcttctgt caaagacaac ggtctcaatg cccttctccc tgcatgcagc
       61 ggcaagacgc tttcctacca ggacagccac ctcactcttt ttcttcccgg ttacagaggc
      121 aatctccttc tcccttgagg aagctgcagc cagtgtcacg ccattgacgt cgtcaacggc
      181 ctgggcatat atctgtttgt tgctgcggaa gaccgccacc cttggttttt cggccgttcc
      241 cctgatccgc ttgcggattc tcagtttaat tttttctctc ttttctgtct ttgctaaagc
      301 catgacttca atatttaatc atgttacaca ccagccgatt taccggcctt tctcctgagc
      361 tgttcgccca cgaatttgat acccttgccc ttatacggct caggcggacg cagtgacctg
      421 atctttgctg cgacatggcc tatgagctgc ttgtcaatgc ttgagagggt gatgaccggg
      481 ttgctccttt tttcagtctt ggtttcaacc ttgatctcct gcggcagttc catcacaata
      541 tcatgtgaat aaccgaggct catttcgaga cgttggccat tggcttctgc acggtaaccg
      601 acacctacca gctcaagatc cttgcggtag ccttttgaaa cgcccgtcac catgttggcg
      661 atgagagcac ggtagagtcc gtgaagcgat ttaaggcgct tttcgtcgtt gggtcttgcc
      721 agtattattg tattgttttc cacctccact ttgatatcac tgtcaacctt ctgtgacagg
      781 gttcccaggg ggcctttaac gcttaccaca ttgtcgtcac tcactgaaag ggtgacgcca
      841 gatggcaggt ttacaggtaa ttttcctatt cgtgacattt gatttcctcc tgtttagtaa
      901 acataacata ttacttcacc tccgacattc tccttcgctg cctccttttc agtcattaca
      961 ccgcgggagg ttgagagaat ggctattccc agcccgttga ggacacgcgg gagttcatca
     1021 acaccggcat atctcctgag gcccggacgg ctgacccgct gtatctgttt gattgcgggg
     1081 agcttggtct tgggatgata cttgagggcg atctttatct tgccctgcag gtcatcatca
     1141 gtgaatttat agctgaggat atatcccttg tcgtaaagta tccgtgtgat atccttcgtc
     1201 agaccggcag ccggtgtctc aaccacttta tggccggctc tgatggcatt ccttaccctg
     1261 gtaagaaaat ctgctattgg atctgtcatc ttatcgtatg attaatttgt ttatatctcg
     1321 gttgttacca gcttgccttt cttacgcccg ggatgagtcc ttttgaagcc atctccctga
     1381 atgtaatcct gctgatgcca aactgtctca tgtagcctct cggtctgccg gtgagtttac
     1441 acctgttgcg ctgcctgatt gggtttgagt tgcgcgggag ggtgctcagg gcaacgtaat
     1501 tgccttctgc tttgagagcg gcccttttgg cggcatacct tttggcaagt ttcgcgcgtt
     1561 tgacttcgcg ggctttcatt gattccttag ccatattccg atctgttatt tgtttttaaa
     1621 gggaattccg aattctttaa gcagggcata agcctcctcg tcactctttg ctgttgtaac
     1681 gaatgtgatc tgcagtccca tgatcttggc gatcttgtcg aggtctatct cggggaagat
     1741 gatctgctcg gtgatgccga gggtatagtt accgcggccg tcgagttttg cactgacacc
     1801 cttgaagtca cgtatgcgcg gcagggctac agagatgagc ctctcgagga attcgtacat
     1861 gcggtcctgg cgcagggtaa ccatcacgcc gataggcatg gctttcctga gcttgaagtt
     1921 tgatatgtcc ttcctggaga tggtctgcac cgccttctgt ccggcgatat ctgatacctc
     1981 cttctgtccc atttcaagca gtttcttgtc ggcaatggcc tggccaagtc cctggttgat
     2041 gactattttt tcaagacgcg gcacctgcat cacagttgta taattaaact gcttcacaag
     2101 ggcaggtgtg atctcattct tatatttggt tcttaaagca ggcgtataca ttacttgatc
     2161 tcctctcctg attttttggc ataacgtacc agtttgttct tgtcattcag cttgcggccg
     2221 acgcgggtag gtttaccggt tgccgggtca atgaccatga ggttcgagat gtggatgggg
     2281 ccctcctttt tgagaatacc tccctgggga gcctttgcgt tgggtttggt atgttttgat
     2341 accatgttga tcccttccac cagggctttg tttttggccc tgtcgacctc gaggacgcgt
     2401 cctttctgtc ctttggattc accggcaatg acagccactg tgtctccctt cttaatgtgt
     2461 aattttttct gcatcgggtg tcagattaac agttataaca cttcaggtgc aagtgaaacg
     2521 atcttcatat acttgtcgcg cagttccctg gcgacagggc cgaagatgcg ggttccgcgg
     2581 acttcatcct ggttggtgag cagcaccacg gcgttgtcat caaagcggat ataggaaccg
     2641 tcagcgcggc ctatttcctt ggtggttctt actaccactg ccctgctgac agagcctttc
     2701 tttgcttcac ctgaggggag agcgctcttg acggagacga caatcttgtc acccacgctt
     2761 gcatacctct tcttgcttcc gccgagcacc ctgatgcaaa gtacctcctt cgcaccggta
     2821 ttatcggcta cattcaacct gctttcctgc tgtatcatga ttacttagct ctttcaatta
     2881 tttcaaccaa tctccaggac ttgtttttgc tgaggggcct ggtttccatt atgcgcaccg
     2941 tatcaccgat gttgcagctg ttctcctcat cgtgagccat gagcttcttt gtcttgctga
     3001 cgaatttgcc atagatcggg tgtttaacct ttctgtgaat ggcaacgacg atagatttgt
     3061 ccatcttgtt gctgacgaca acacctgtac gttcttttct aaggttcctt tcttccatgc
     3121 tgatacggct attttgattt ctcattgatc tctctcttgc gcatctctgt catgagcctg
     3181 gcaactgttt tcctgctctc cttgatcttc tggggatttt caaggggaga gattgcgtgg
     3241 ttgagtttca tgcgcacgag tgcggtcttc tcgttgtcaa tacgttcaag aagctcctgg
     3301 ttgcttaatt ctcttatctc tgaacttttc attgctctgt ttttttattc ctctgcgtaa
     3361 tcatgacgca ccacgaattt tgttgccacg ggcagttttg cagctgcgag gcggagtgct
     3421 tccttggcca cgtcaaaggg aacgccttcg gcttcaatga tgatgcggcc tggggtaacg
     3481 ggaaccacga atccttcagg tgcaccctta cctttaccca tacggacctc tgcaggtttc
     3541 ttggttatcg gtttgtcagg gaagacacgg atccatatct ggccttcacg tttcatgtga
     3601 cgggtcacag cctggcgtgc agcctcaagc tgacggccgg taatccatgc ctcctcgagg
     3661 gttttgatgc caaatgatcc gaatgccagc tgattgcctc tctgggcatt gcctttcatc
     3721 ttgcccttct ggctcctcct gaacttggtc ttcttcggtt gtaacatcgc tatataatat
     3781 taactgttat ttacttatct cgattacctc ttgtttccgc gatcgcctcc acggtcgcca
     3841 cggtcaccac ggtcaccacg atcacggtca ccacggtcgc cgcggtcacg gtcgccgcgg
     3901 tcgctcttct tcctgaagcc gcctgagggt gccttgcctc ccttgacgtt cttcgcgc
//
LOCUS       DAOPXU010000173         3806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_31840_length_3806_cov_13.194615, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000173 DAOPXU010000000
VERSION     DAOPXU010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31840_length_3806_cov_13.194615"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..799
                     /locus_tag="PKK03_11940"
     CDS             122..799
                     /locus_tag="PKK03_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX85151.1"
                     /translation="MNDKKKILAVDDEEPILEILSFNLEGEGYQVDTCLSAEDAMRKN
                     LHTYDLFILDVMMGHVSGFRLAEKIRKENDLSTPIIFLTARDTENDRITGFNLGADDY
                     LAKPFSVRELMARVKAILRRSAPARVTMETAAGSFTVDTENNLILTDGRALELTRKEY
                     DILSLLIRNEGKIFPRHEILARVWGGDVIVTDRTVDVTIARIRKKLGEKGNMIRNKSG
                     YGYYLGE"
     gene            812..2572
                     /locus_tag="PKK03_11945"
     CDS             812..2572
                     /locus_tag="PKK03_11945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNX85152.1"
                     /translation="MTKSISFRRRLFLYYFGIFLLFTVLILAFQYHREKDIKISALDN
                     RLNDMAQLVDNYIKVNSLSETGNYRLVDSVFNLIPVPDLRITVLAIDGKVLYDSSVDD
                     WSTMENHLNRPEVGKSLFSPYGTAVRHSESTGKDYYYFSRYFNTHYVRLAEEYNINIR
                     GFLKREQIFLVFMAIVFAGIWALLAAVTRRMGKSITMLRDFAARMRRGEGNDPSIIFP
                     KNEIGETGKEIVRIYNNLALNTAELSLQKEKLIRHLHVLNEGVAFFTPERTVTLSNSH
                     FMVFLNAITGEHSLTPEGFIDHPEFAGIRTFLNSNLETRAGKQENPSTEMRIEKGGRY
                     FSVRCILFNDKSFEIVITDITRTEQNKAMRQQMTSNIAHELKTPIASVRGYLETLIVN
                     KDLDEEKRNHFLDKALGQAARLTDLINDIVTLNKLDETGSNFPFSEVDVADVVREVCD
                     NLMTDIRSKQMKVDIAIEPGTMVNGNRSLIVSVFQNLLENAITYAGEKVTVTVRTLPG
                     ESGFRTFSFADNGIGIPAEHQERIFERFYRIDDGRSRKSGGTGLGLAIVKNAVLLHRG
                     EISVRTRPGGGAEFIFSLPA"
     gene            complement(2641..3702)
                     /gene="selD"
                     /locus_tag="PKK03_11950"
     CDS             complement(2641..3702)
                     /gene="selD"
                     /locus_tag="PKK03_11950"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNX85153.1"
                     /translation="MTTTVNGPAGFDLLSTVETGGCSAKIPPRQLEELLQDMPLPADP
                     RILVDIATHDDAGVYKINDETALVITTDFFPPVVSDGYEFGLIAATNSISDVYAMGGT
                     PLVALNIMMFPSTRIPMEVYKEILRGGHDKAREAGVSIIGGHTIDDYPPKYGLAVIGT
                     VHPGRVITNAGVREGDELILTKPLGTGIILAGHRMKMTIPSQLENAVSIMKQLNARGA
                     ELMQKYSVRGATDITGFGLAGHALKMARGSGVTVNIDMKSVPLLDCVYSLADQGCIPG
                     AAFRNLDYTEGGTRFTSDLDYNLKMIAHDAQTSGGLLMAVAPEKSRELLKELHESGHG
                     EAAVIGKAIRKAEADLVLQ"
BASE COUNT          948 a         1024 c          962 g          872 t
ORIGIN      
        1 tctgaaggtc tgaaggtctg aaggtctgaa ggtctgaagg tctgaaggtc tgaaggtcga
       61 aattagcgca cacaaggagt agacatggca tagcttgccc atagggcatc aagcatttta
      121 aatgaacgac aaaaagaaaa tactggccgt tgatgatgag gaaccaatcc tcgaaatcct
      181 ctcattcaac cttgagggcg aaggctacca ggttgacacc tgcctttcgg ccgaggatgc
      241 catgcgcaaa aacctccaca cctatgacct gttcatcctc gacgtgatga tgggccatgt
      301 gtcagggttc cgcctggcgg aaaagataag aaaggaaaat gacctctcaa ccccgataat
      361 cttcctcact gcaagggaca cggaaaatga ccggatcacc ggtttcaacc tcggagccga
      421 tgactacctt gccaaaccat tcagcgtcag ggaactgatg gcgcgggtta aagccatcct
      481 ccgccggtct gcgccggcac gggtgaccat ggagacagcg gccggctcct tcaccgttga
      541 cactgaaaac aacctcatcc tcacagacgg ccgggcactc gagcttaccc gcaaggagta
      601 cgatatcctc tccctcctca tccgcaatga aggcaagatt tttcccaggc atgagatact
      661 ggcaagggtc tggggcggtg acgtcattgt caccgaccgt accgttgacg tgaccatcgc
      721 ccgtatccgc aaaaaactgg gcgaaaaggg taacatgata agaaacaaat cagggtacgg
      781 ttactattta ggcgaatgaa aaaccagtca aatgacaaaa tcgataagct tcaggcgtag
      841 actgttcctt tattatttcg ggatcttcct cctctttacg gttctgattc tggcattcca
      901 gtaccaccgt gaaaaagata tcaaaatctc ggctcttgac aacaggctga acgacatggc
      961 tcagcttgtt gacaactaca taaaggtcaa ttcgttaagc gagacaggca actatcgcct
     1021 ggtggactcg gttttcaacc ttattccggt accggacctc cggatcacgg tattggcaat
     1081 tgatggcaaa gtgctgtatg acagctcggt ggacgactgg tcaacaatgg agaaccatct
     1141 caacaggccc gaggtgggca aatcgctctt ctctccctac ggtactgcag tccggcactc
     1201 ggaatcgacc ggcaaggact attattactt ttcgaggtac ttcaacacac actatgtcag
     1261 gcttgccgaa gaatacaaca tcaatatcag gggattcctg aaacgtgagc agatttttct
     1321 cgttttcatg gccattgtct tcgcaggtat ctgggctctg cttgctgccg tgacgcggcg
     1381 gatgggaaaa agcattacca tgctacgtga ttttgcagca agaatgcgtc gcggtgaagg
     1441 caatgacccg tcaattatct tcccgaagaa tgagatcggt gaaacaggca aggaaatagt
     1501 caggatctac aataacctgg cactcaacac cgctgaactg tcactccaga aagaaaagct
     1561 catcaggcac ctccatgtgc tcaatgaggg agtggcattc ttcacccctg aacgtactgt
     1621 gacactctcg aacagccatt ttatggtgtt ccttaacgcc atcaccggag aacactcact
     1681 cacacccgag ggtttcattg atcacccgga attcgcaggc atcaggactt tcctgaacag
     1741 caatctcgaa acaagggccg ggaagcagga aaatccgtcc accgagatga ggattgaaaa
     1801 agggggcaga tacttctccg tgagatgtat cctgttcaat gacaaaagct tcgagattgt
     1861 catcaccgat attacccgca ccgagcagaa caaagcgatg cggcagcaga tgacctccaa
     1921 catcgcccat gaactcaaaa ctcctattgc gtctgtcagg ggatatctcg aaacactgat
     1981 cgtgaacaaa gacctggatg aggagaagcg taatcatttc cttgacaaag cccttggcca
     2041 ggctgcccgc ctcaccgacc tgatcaatga cattgttacc ctgaacaagc ttgatgagac
     2101 aggcagcaac ttcccgttca gtgaggttga cgttgcagac gttgtaaggg aagtctgcga
     2161 caacctcatg actgacatca ggagcaaaca gatgaaggtc gatattgcca ttgagcctgg
     2221 caccatggtc aacggaaaca ggtcactgat cgtatcggta ttccagaacc tccttgagaa
     2281 tgccatcacc tatgccggtg agaaagtcac cgtaacggtg aggaccctcc ccggtgaatc
     2341 cggtttccgc accttctcat ttgctgacaa tggcatcggt attcctgcgg agcatcagga
     2401 gaggatattc gaacggtttt acaggattga cgacggccgt tcgcgcaaga gcggaggcac
     2461 aggtctcgga ctggctattg tcaagaatgc tgtattgttg cacaggggag agatctctgt
     2521 caggacacgc ccgggtggcg gagcagaatt cattttctcg ctgccggcat agacaggact
     2581 tgttcagccg gcaggcgatg taacgccaaa tgcatcagct ttcacgatcc cggcagcttt
     2641 ttattgcagc acaagatcgg cctctgcctt cctgattgct ttaccgatga ctgcggcctc
     2701 accgtggcct gactcatgca gttccttcag cagttcccgg ctcttctccg gcgccaccgc
     2761 catcagtaac ccgcccgagg tctgggcatc atgagctatc atcttcaggt tatagtcaag
     2821 atcactggta aaccgtgtcc ctccttcggt gtaatccagg ttgcggaacg ccgcgccggg
     2881 tatgcacccc tggtcagcca ggctgtaaac acaatcgagc agcggcacgc tcttcatgtc
     2941 aatatttaca gtcactccgg aacctcgtgc catcttaagg gcatggcctg ccaggccgaa
     3001 accggtaatg tcggtggcac ccctgacgct gtatttctgc atcagctccg caccacgggc
     3061 gttgagctgc ttcatgatgc tcacggcatt ctcaagctgc gaaggtattg tcattttcat
     3121 cctgtgtccc gccaggatga taccggtacc caggggtttg gtcagtatca gctcatcccc
     3181 ttcccttacc ccggcattgg tgatgaccct gccgggatgc accgtgccga tcacggccag
     3241 cccgtattta ggcgggtaat catcgatggt atgtcctccg atgatgctga ctccggcttc
     3301 gcgtgccttg tcatggcctc ccctcaggat ttccttgtat acctccatcg gaatccgggt
     3361 tgagggaaac atcattatgt tcagcgccac caggggtgtt ccgcccatgg cgtaaacatc
     3421 acttattgag tttgttgcag ctataagacc aaattcgtaa ccgtcgctca ccaccggcgg
     3481 aaagaagtcg gttgtaataa caagggctgt ctcgtcattg attttgtaaa cgcccgcgtc
     3541 atcatgggtt gcaatatcga ccaggatgcg cggatctgca ggcagaggca tatcctgcag
     3601 cagctcctca agctggcggg gaggaatttt tgccgagcag cctcccgttt cgacagttga
     3661 gaggagatca aatccggctg gtccgttaac agtggttgtc atatggcatt ttccctgtca
     3721 gggacggata atatgatccg catccttcag cacgttcagt atctggtaca tgtcactgaa
     3781 gcttcccgcc ggcaggcggt cacgga
//
LOCUS       DAOPXU010000174         3730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_32796_length_3730_cov_14.401905, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000174 DAOPXU010000000
VERSION     DAOPXU010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32796_length_3730_cov_14.401905"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="PKK03_11955"
     CDS             complement(<1..1174)
                     /locus_tag="PKK03_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNX85154.1"
                     /translation="MNTRTLWIGLVLVTLLSFSVLLFFGKEIYRLKPPIPEKVVSDSG
                     EVLFTAQQIKDGQNVWQSIGGQQVGTVWGHGAYLAPDWSADWLHRESLTILGGWSESE
                     FGLSFDKIDKEKQAMLEVRLQKALRANTYDQQTGILTLTADRVQAVNGNAAYYAGLFM
                     NDPALESLRESYSIPANTVKEKGRMDAMNAFFFWAAWACVTERPGSELTYTSNWPPDP
                     VIGNKPSAGVVLWTGFSVIMLLVGVGLLAYYNARNRSDVIDRSLLPQDDPLGGLNPTP
                     SMKAVLKYYWVVVGLILLQILTGVITAHYGVEGSGFYGLSLDSIMPYSISRTWHIQLA
                     LFWIATAWLAAGLYIAPAVSGHEPKFQKFGVNLLFFALLVIVLGSMAGEWMGVMQKL"
     gene            complement(1290..1973)
                     /locus_tag="PKK03_11960"
     CDS             complement(1290..1973)
                     /locus_tag="PKK03_11960"
                     /inference="COORDINATES: protein motif:HMM:NF039786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HNX85155.1"
                     /translation="MKKLKLRNSNGAGVNGNPRGLFSRSGRLAILTALLMLLSTGITL
                     AHCDTMEGPVIKDAKLALDVNNINYALKWIRETDEAELREAFALTMKVRKLGPEAQTL
                     ADRYFFDTLVRIHRNGEGVGFTGVKPAGTPVDERILSADKAIEAGNLSPLAGKVPEEM
                     KHELHIRFDKVMSLKNFDVNNVRAGREYVAAYVSFFKFAEGEEHEHAAEHSETVAGAA
                     PAPACGHGH"
     gene            complement(2122..3249)
                     /locus_tag="PKK03_11965"
     CDS             complement(2122..3249)
                     /locus_tag="PKK03_11965"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNX85156.1"
                     /translation="MKYAFLIAAFNAFFFTALLLQKKTSALHDRILVLWLIYLGLFIG
                     IYAFYSHELFTRFLLLSVSLISLLMLHGPFLYTYVLTLVTGRTRITAKDSLHLIPFLL
                     FNAYILTASFIPELSARLNLEKVTPGQGTPLLFSFFLILTALSGTVYFLMTMKLFRKL
                     DTNISNNFSNAEGVDLHWIRRLTIAFGIIWTILITITVIHHILNLFSAAFCTDGLFLT
                     LSVFVILIGWFGLRQRVIFSPGSMVLSTDAAANQARYSGSRLSDADAGAFAARIREYM
                     DSSGPYLEPELSLPQLASDLDIPVHYMSQVINEQFGQNFHDFINRYRVDAFRKRLASG
                     GSDNLTLLGIAFECGFNSKSAFNRVFRQFTGMTPSEYKKTL"
     gene            3468..>3730
                     /locus_tag="PKK03_11970"
     CDS             3468..>3730
                     /locus_tag="PKK03_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85157.1"
                     /translation="MKIIWKYLKPFKKWLFLSMGLAAIAQILELIDPIIFGKIIDNFA
                     VNTDGRNQQELVNGVLRLLALAVGIALLARLAHAFTDYFTRLVV"
BASE COUNT          941 a          919 c          944 g          926 t
ORIGIN      
        1 ccagcttctg catcacaccc atccattcac ctgccatcga gccaagaact atcaccagca
       61 gggcaaaaaa gagaaggttg actccgaatt tctggaactt cggctcatgc cctgaaaccg
      121 caggggctat gtatagtccg gctgcaagcc atgcagttgc tatccagaag agcgccagct
      181 gtatgtgcca tgtcctggag atagagtatg gcatgatact gtcaaggctc agcccgtaga
      241 aaccgcttcc ttcaacaccg tagtgtgctg tgataacacc ggtgagtatc tggagcaata
      301 ttagtcccac aactacccag taatatttca gtactgcctt cattgacggg gtgggattca
      361 gtccgcccag cgggtcatcc tgcggcagca gtgacctgtc aatcacatca gacctgtttc
      421 ttgcgttgta gtatgcaaga agtcctactc cgacaagcaa catgataacg ctgaatcctg
      481 tccagagtac aacgcctgca gagggtttgt tcccgataac aggatccgga ggccagttgc
      541 ttgtgtaagt cagctccgac cccggccgtt ccgtcacgca ggcccatgct gcccagaaga
      601 agaaggcatt catggcatcc atgcgcccct tttccttgac tgtattggca ggtatggaat
      661 acgattccct caggctttca agtgccggat cattcatgaa gaggccggca taataggcag
      721 cgttgccatt caccgcctgt accctgtcgg cagtgagcgt aagaatgcct gtctgctgat
      781 cataggtgtt tgcacgaagg gccttctgaa gcctcacctc cagcattgcc tgtttctcct
      841 tatcgatctt gtcaaaagaa aggccgaatt cactttcaga ccacccgccg agaatggtca
      901 gtgattcacg gtgcagccag tcggcagacc agtccggggc aagataggcg ccgtggcccc
      961 agacggttcc aacctgttgg ccgccgattg attgccagac attctggccg tcctttatct
     1021 gctgtgcggt aaaaagaacc tcgccactgt cagagacaac cttttcagga atggggggct
     1081 tgagcctgta aatctctttt ccgaagaaca gcaggacgga aaagctgagc agtgtgacca
     1141 gtacaagacc gatccataga gtccttgtat tcataatcag gtatttaggg gttaattaat
     1201 gaatcaggtt caagatctca tggacgtaaa ataggcaatt aattcatgcg agtcaattga
     1261 ttgtgatatt tatgaccact taccttctgt cagtgcccgt gtccgcaggc cggtgccgga
     1321 gcagctcctg ccacagtttc agaatgctct gcagcatgct cgtgttcttc gccttcggcg
     1381 aacttaaaga agcttacata agcagccaca tattccctgc cggccctgac gttattgacg
     1441 tcgaaattct taagagacat aaccttatca aagcgaatat gcagttcatg tttcatctct
     1501 tcaggaacct taccggccag cggcgagagg ttgcctgctt cgattgcttt gtcggctgaa
     1561 aggatgcgct catccaccgg ggtgcccgca ggcttgacac cggtgaagcc aacgccctct
     1621 ccgttcctgt gtatcctgac aagcgtgtca aagaaatacc tgtcggcaag ggtttgtgct
     1681 tccggcccga gttttctgac tttcattgta agtgcaaagg cctccctgag ttcagcctca
     1741 tcagtttccc ggatccactt cagggcataa ttgatgttgt tgacgtccag tgcaagcttt
     1801 gcatccttta tcaccgggcc ttccatggtg tcacaatgcg caagggtaat gcccgtcgat
     1861 aaaagcatca gaagtgcggt caaaattgcc agccgtcctg atctgctgaa aagtccacgg
     1921 ggatttccgt ttacccctgc tccgttactg tttctcagtt ttaatttttt catgttgttt
     1981 gattttaaat gattaatgaa aatttcctga ggcaaagcta aaatcagcag aagaggtaat
     2041 cgccccgttt tataaaacgg gacctgtttg atgaggctga attggtgctg ctttacaaaa
     2101 caggacctgg ctgcaggaag attacagggt ctttttgtac tccgaagggg tcatcccggt
     2161 gaattgcctg aagactctgt tgaatgctga tttggaattg aatccgcact caaaggcaat
     2221 tccaagcagt gtcaggttgt ctgatccgcc cgatgccagt cgcttcctga atgcatccac
     2281 ccggtacctg tttatgaaat catggaaatt ctgtccgaac tgttcattga tgacctgcga
     2341 catgtaatgt accggtatat cgaggtcaga ggcaagctgg ggcaatgaaa gctccggttc
     2401 caggtatggc ccggaggaat ccatgtattc cctgatcctg gcggcgaagg ctccggcatc
     2461 agcatcggag agcctggatc cggaatatct tgcctggtta gccgccgcgt ccgtgctcaa
     2521 caccatgctt ccgggtgaga atataacccg ctgcctgaga ccgaaccacc cgatgagaat
     2581 aacgaatacc gacagggtga gaaaaagacc gtcggtgcag aatgctgccg agaacagatt
     2641 cagaatatga tgtattacgg taatggtgat aagtatggtc cagatgatcc cgaatgctat
     2701 cgtaagccgc cttatccagt gaagatcaac accctcggcg ttggaaaagt tgtttgagat
     2761 gttcgtgtcg agtttcctga agagtttcat ggtcatcagg aaataaacag tgccggaaag
     2821 agctgtaagg ataaggaaaa acgaaaagag aagcggagtg ccctgcccgg gggttacctt
     2881 ttccaggttc agcctggccg agagttcagg aatgaatgaa gcggtgagga tatatgcatt
     2941 gaacagcagg aacgggatga ggtgcaggct gtccttcgcg gttatccgtg tccttccggt
     3001 gacaagtgtc agcacatagg tatagagaaa gggtccgtgg agcatcagca gggagatcag
     3061 cgatactgag agcagaagga acctggtaaa cagctcatgc gaataaaagg cataaatccc
     3121 tataaacagc ccgagataga tcagccacag aaccagtatc ctgtcatgca gtgcacttgt
     3181 tttcttctgc aggagcagtg ctgtaaagaa gaacgcgttg aatgccgcta tcaggaaggc
     3241 gtatttcata cctcaaaaat agtcaattag cattaccggt ctgaggtgat tgtcctcccg
     3301 ttttcaattt gtaaattgcg tatcctgacc cggaaacaga attaccggaa attgcgtcac
     3361 tgtcacgacg ggatgacctg ccggtaatca taacaccccg gagatgccct gtggagagaa
     3421 tttagtttct atctttagtg attgttccct gatgaaagac cagagttatg aagatcattt
     3481 ggaaatacct gaaacctttt aaaaaatggc tttttctgtc aatgggactg gcagccattg
     3541 cgcagatcct tgaactgatt gacccgatca ttttcgggaa aatcatcgac aattttgctg
     3601 taaacaccga cggccgcaac cagcaggaac tggtgaacgg ggtactgaga ctgctggccc
     3661 ttgcagtggg aatcgccctc ctggcgaggc tggcccatgc cttcaccgat tattttacac
     3721 gcctggttgt
//
LOCUS       DAOPXU010000175         3690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_33329_length_3690_cov_12.474553, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000175 DAOPXU010000000
VERSION     DAOPXU010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33329_length_3690_cov_12.474553"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PKK03_11975"
     CDS             complement(<1..539)
                     /locus_tag="PKK03_11975"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX85158.1"
                     /translation="MLDRYNRHINYLRISVTDRCNLRCRYCMPEEGVKLIRHEDILTY
                     DEITAFTRVAVAAGINKVRITGGEPLVRKGVTELVRMIADIDGVTDLSMTTNGILLAG
                     YATALAEAGLMRVNVSLDTLDPVKYGYVTRGGDLYSVLKGIEAAQKAGLRPVKINCVI
                     KSTPDESDAREVAEYCMREG"
     gene            complement(523..1698)
                     /locus_tag="PKK03_11980"
     CDS             complement(523..1698)
                     /locus_tag="PKK03_11980"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNX85159.1"
                     /translation="MITFEEAFRIVTESSFRLGREVVSLSEAAGRALTLPVRSDMDMP
                     PWNKSAVDGYACRHTDLGHELTVLETVAAGVMPQKEVVPGTCSRIMTGAAVPAGADYV
                     FMLEDSEELSDGRVKFTGRAGNDNIAKAGEDIMKDQTVLRSGHYIKPQDVAVLASVGA
                     MEVTVSCRPRIGVISTGDELVEPDIVPAGAQIRNSNASQLMAQIARAGARGKYYGIAR
                     DDVESITSLLKKALEENDVVLISGGVSFGDFDLVPRVMRSLGLTIHFDQVAMQPGKPL
                     TFCTGDKKAVFGLPGNPVSVFVQFEVMVRAFLDRMAGVSEQAPVLQMPLAADYSRKKA
                     ERMSWIPCTITAAGEALPVEYHGSAHISALTGAWGVIIIPRGQSWIQKGEMVSVRQV"
     gene            complement(1705..2781)
                     /locus_tag="PKK03_11985"
     CDS             complement(1705..2781)
                     /locus_tag="PKK03_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX85160.1"
                     /translation="MMLRTEKLCKRLDAFLLQEISLEVSKGDYYVLLGRSGAGKTQLL
                     ELICGLTPPDKGKIFLDGEDITRKRVQDRGIGLVFQDLALFPHFSVKDNIMYPLKVMK
                     LPLKERVDRVQRTAGEMNITDLLLRKPENLSGGEKQRVTLARTLVTQPKILLLDEPMA
                     SVDASLKDSIRRMLRRLNRNGQTIVHVTHDFGEAISLASRVGVMHNGRIIQEGTPDEV
                     FTHPANRFVARYAGIKNFFRVTFVRSYNCCSCITDNRTKFKLPKGDYPSSGLLIIAAT
                     EVLLSRQGAAGVQINNIHGVIEDIVPSPLGYEITINAGDHFYADITRREMDEEKYVTG
                     EPVIISFRPESLKMVGEGEEMNKY"
     gene            complement(2765..3574)
                     /locus_tag="PKK03_11990"
     CDS             complement(2765..3574)
                     /locus_tag="PKK03_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX85161.1"
                     /translation="MKGHTLFNWIFALLASLVLLFIIAPLAGMFLHTSTEEMRSTASD
                     PAVIRSVWYTLAIAFTATIIFAVGAVPLAWIMARRKFPLKNVVQGIIDLPVVLPHTAA
                     GIALLGFISRDGFLGGAAQKAGILLVNNGAGIALAMAFVSLPFLINAARDGFAAVPER
                     TEKAALMLGATQSRIFFTISLPQAWRSVVSGFIMMFARGMSEFGAVVIIAYHPMIAPV
                     LIYDRFNTYGLSAARPASIIFILVALFFFVLFRVLATERRQIFRREDDATD"
BASE COUNT          807 a         1057 c          970 g          856 t
ORIGIN      
        1 ccctctctca tacaatactc tgcaacctcg cgggcatccg actcgtcggg cgtactcttt
       61 atgacgcaat tgatcttgac aggacgcaat ccggccttct gtgcagcttc aattcctttt
      121 aaaacagagt aaaggtcacc gccgcgggtg acatatccgt atttgacggg gtccagagtg
      181 tcaaggctga cattgaccct catcagtcct gcctctgcca gagccgtggc ataaccggca
      241 agcagaatgc cgttggtggt catcgagaga tctgtcaccc cgtcgatgtc tgcgatcatg
      301 cgaaccagtt cagtcacccc cttccttacc agcggttcac ctccggtgat gcgcaccttg
      361 tttatacctg cagccactgc cacccgggtg aaggcagtga tctcgtcata ggtgagtata
      421 tcctcatgcc tgataagttt cactccctcc tccggcatgc agtagcggca acgcaggtta
      481 caacggtcgg tgaccgagat gcgcagatag tttatatgtc tgttatacct gtctaacact
      541 taccatttct cctttctgaa tccagctctg tccgcggggt atgataatga cgccccaagc
      601 tcccgtcagg gcagaaatgt gtgccgagcc gtggtactca accggcagtg cctcgccggc
      661 tgcggtgatg gtgcatggta tccatgacat cctctccgcc ttcttgcgtg agtagtcagc
      721 cgccaggggc atctgaagca ccggggcctg ttcagagact cccgccatcc tgtcgaggaa
      781 tgcccttacc atcacctcaa actgcacgaa gacggagacc gggttacccg gaaggccgaa
      841 tacggctttc ttatcccctg tgcagaatgt aagtggtttg ccgggttgca tggcaacctg
      901 gtcaaagtgt atggtcagtc ccagcgaacg catcacccgg ggaacgaggt caaaatcacc
      961 aaacgagaca ccgccggaga ttagcaccac atcgttttcc tcgagtgctt tcttcagcag
     1021 ggatgtgatg gattccacat catcacgggc aatgccgtag tatttacccc tggcgcctgc
     1081 cctggctatc tgcgccatga gctgcgatgc attgctgttg cgtatctggg ccccggccgg
     1141 gacaatgtcg ggttccacca gttcatcacc tgtgctgatc actccaatcc ggggacggca
     1201 gctgacggtt acctccatgg cacccacgga agccagcact gctacgtcct gcggtttaat
     1261 ataatgaccg gagcgcagaa cagtctgatc cttcattata tcttctcccg cttttgcaat
     1321 gttgtcgttc ccggccctgc cggtgaactt tacccgtccg tcagaaagtt cctctgagtc
     1381 ctcgagcatg aacacgtaat cagcccctgc gggcacggca gcgccagtca ttatgcgtga
     1441 acatgtgccc ggcacaacct ccttctgagg catgacgcca gcggcaactg tctcaagcac
     1501 cgtgagctca tgtcctagat ccgtatgacg gcaggcatag ccgtcaacgg cgctcttgtt
     1561 ccagggaggc atgtccatgt cactcctcac cggcaacgtc aaagcacgtc cggcagcctc
     1621 tgaaaggctg acaacctccc ttccgagacg gaatgatgat tccgtcacaa tcctgaatgc
     1681 ttcctcaaat gttatcatat catttcagta tttattcatt tcttctcctt cccctaccat
     1741 tttcagcgat tcaggacgga acgaaatgat taccggttct cctgttacat atttctcttc
     1801 atccatctcg cggcgcgtga tgtcagcata aaagtgatcg ccggcattga tggtgatctc
     1861 gtagcccagg ggggaaggta cgatgtcttc gatgacaccg tgaatattgt ttatctgcac
     1921 gccggctgca ccctgcctcg agagaagtac ctcggtggca gcaattatca gcagccccga
     1981 agaagggtaa tctcctttcg gcagtttgaa tttcgttctg ttgtcagtta tgcatgagca
     2041 acagttatat gatctgacaa aagtgacccg gaagaagttt tttattccgg cgtagcgggc
     2101 cacgaacctg ttggccgggt gagtgaatac ctcgtcaggg gtcccttcct ggattatccg
     2161 tccgttgtgc atcacgccca ccctgctggc aaggcttatc gcttcgccga agtcatgggt
     2221 gacatgcact attgtctgac cgttcctgtt gagacggcgc agcatccgcc tgatgctgtc
     2281 cttcaggctg gcatccactg aggccatcgg ctcgtcgaga aggagtatct tcggctgcgt
     2341 caccagtgtc cttgccagcg tcactctctg tttttccccg cccgagaggt tctcaggctt
     2401 gcgaagaagc aggtcggtaa tgttcatctc cccggcagtt cgctgaaccc tgtcaaccct
     2461 ctccttaaga gggagcttca ttaccttcag ggggtacatg atgttgtcct tcactgaaaa
     2521 atgcgggaag agggccaggt cctggaatac cagtccgatg ccacggtcct gtacccgttt
     2581 gcgggtaata tcctcaccat ccaggaatat ttttcccttg tcggggggag tgaggccgca
     2641 gatgagctcg aggagctgtg ttttgccagc gccggagcgg cccagcagca catagtaatc
     2701 accctttgac acctccagtg atatttcctg aagcaggaag gcatcgagcc ttttgcacag
     2761 tttttcagtc cgtagcatca tcttccctcc tgaatatctg gcggcgttct gtggcgagca
     2821 ccctgaagag cacgaagaag aacagggcca caaggataaa tattattgaa gcgggccttg
     2881 cggcgctgag gccataggta ttgaagcggt catagatcag cacaggggca atcatggggt
     2941 ggtacgcgat gatcaccaca gctccgaact cgctcattcc gcgggcgaac atcatgatga
     3001 agcccgacac caccgaccgc catgcctggg gcagcgagat ggtaaagaat atgcgcgact
     3061 gggttgcgcc gagcatcagc gccgccttct ctgtacgttc cggtaccgct gcgaagccgt
     3121 cgcgggcagc attgatgagg aagggaagac tgacgaaagc cattgccagg gctataccgg
     3181 caccgttgtt gacaagcagt atgcccgctt tctgagctgc accgcccagg aatccgtcac
     3241 gtgagatgaa acccagcaat gctattcccg ccgccgtgtg agggagcacc acaggcaggt
     3301 caataatacc ctgcacaaca ttcttcaggg ggaacttcct gcgtgccatg atccatgcca
     3361 ggggcacggc gccaactgca aagatgattg tcgccgtaaa ggcgatggcc agggtgtacc
     3421 acacactgcg tatcacagcc ggatctgagg cagtgctgcg catctcttca gtggaggtat
     3481 gcaggaacat gccggccagc ggtgctatga taaagagaag caccaacgat gccagcaatg
     3541 caaagatcca gttgaagagc gtatggcctt tcatagcatt gattactttg ccgggacggc
     3601 agccatgaat ttcctcagtg atgaaggcac tgcatcagtg tcatcggcaa cggcaggaac
     3661 aagcgggtcc tggccacact tcctgaagat
//
LOCUS       DAOPXU010000176         3588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_34756_length_3588_cov_9.339655, whole genome shotgun sequence.
ACCESSION   DAOPXU010000176 DAOPXU010000000
VERSION     DAOPXU010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34756_length_3588_cov_9.339655"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..501
                     /locus_tag="PKK03_11995"
     CDS             76..501
                     /locus_tag="PKK03_11995"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNX85162.1"
                     /translation="MKILILCTGNSCRSQMAHGFLQSFDHNLHVRSGGTEPAARINPK
                     AVEVMADLGIDISHHSPHLVDEYFDEEWDYVITVCDHANETCPAFFGMVKNRLHLGFE
                     DPSHATGTPEFIHSEYIRIRDQIRETFLQFYNEYISNKS"
     gene            498..1289
                     /gene="arsM"
                     /locus_tag="PKK03_12000"
     CDS             498..1289
                     /gene="arsM"
                     /locus_tag="PKK03_12000"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNX85163.1"
                     /translation="MNPAELKLIVQKKYGKIASSDEASQDSCCSTTGCCSTEKISTFN
                     ESYDHLDGYVPDADLSLGCGVPVDYADIRHGDRILDLGSGAGNDCFVARALTGETGHV
                     TGIDFTEQMLLKAEENRKKLGYSNVEFLRGDIEEMPLPDNSYDVVLSNCVLNLVPDKQ
                     KAFSEIFRVLKSGGHFSVSDVVVKGNLSERARSDAELYAGCVSGASTMNQYLTFIKKA
                     GFNDINFHKQKRIELPEGIIDPTEDQDDNGVYSITISAIKPGIIS"
     gene            1361..2416
                     /gene="arsB"
                     /locus_tag="PKK03_12005"
     CDS             1361..2416
                     /gene="arsB"
                     /locus_tag="PKK03_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNX85164.1"
                     /translation="MPSKKRLKLLDRYLTLWIFLAMAAGVIIGWLSPEIAGFWNNLSS
                     GTTNIPIAIGLIVMMYPPLAKVKYEELADVFRNTRVLGLSLVQNWIIGPVLMFLLAIL
                     FLRFNIDYMYGLILIGLARCIAMVIVWNDLAKGDTQYAAGLVAFNSIFQVLFFSVYAY
                     FFIAVLPGWFGLPVNDAVEQITIGQIAKSVFIYLGIPFIAGFLTRFILIRVKNKEWYH
                     TKFIPKISPLTLIALLFTIFVMFSLKGEYIVRIPLDVVRIAIPLVIYFVIMFFVSFFM
                     SRRAGANYEQTATLSFTAASNNFELAIAVAIAVFGINSGEAFAAVIGPLIEVPVMIAL
                     VNAALRLKTKYFRQAEA"
     gene            complement(2533..3429)
                     /locus_tag="PKK03_12010"
     CDS             complement(2533..3429)
                     /locus_tag="PKK03_12010"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNX85165.1"
                     /translation="MNILSVFPNILNSLSGFDPGFLSLVNLWITILIIVVLTCFIVSE
                     ITRNYSQVDKLWSLLPPAYAWVTVGSFHTARTVIMAVLVTLWGLRLTYNFHRKGGYNI
                     IPWKGEEDYRWSILRKSSALKGRLRFGAFNLLFISLYQNVVILLFTTPILMAALHPES
                     PLGATDILAAALMITFLITETVADNQQYRFQTMKRSASGGEHEYRDSLNKGFLTEGLW
                     KYLRHPNFASEQAIWISFYFFGVAASGKWINFTLLGPVLLVLLFIGSSIMTENISSSK
                     YPEYSTYQKEVPKFIPRIFRRK"
BASE COUNT          953 a          800 c          881 g          954 t
ORIGIN      
        1 cagaccttca gaccttcaga ccttcagacc ttcagacctt cagaccttca gacctgacaa
       61 aatgatatgc acaaaatgaa aatactgatt ctctgtacgg gcaacagttg ccgcagccag
      121 atggctcacg gcttcctgca atcatttgac cacaatcttc atgtcagatc cgggggtacg
      181 gagccggcag ccaggatcaa tcctaaggca gttgaggtga tggctgacct ggggattgac
      241 atcagccatc attctcctca ccttgttgat gaatattttg atgaggagtg ggactatgtg
      301 atcactgtat gtgatcatgc caatgaaacc tgtccggcat ttttcggcat ggtgaagaac
      361 agactccatc tcggatttga ggatccctca catgccaccg gaacaccgga gttcattcac
      421 agtgaatata tcaggatacg cgatcagatt cgtgagacat tcctgcagtt ttataacgaa
      481 tacatcagta acaagtcatg aatcccgcag aactgaaact tattgtacag aaaaagtacg
      541 gtaaaatagc atcgtcggat gaagcttcac aagactcctg ctgcagtaca accggatgtt
      601 gctcaacaga aaagataagt accttcaatg aaagttacga tcatctcgac gggtatgttc
      661 ctgacgctga cctgagcctt ggatgcggtg tgccggttga ttatgcagat atccggcatg
      721 gtgaccgtat tcttgatctt ggcagcggtg ccggcaatga ctgtttcgtt gccagggcgc
      781 tgacgggtga gacaggtcat gtgacaggta ttgattttac agagcagatg ctgttaaagg
      841 cagaagagaa caggaaaaaa ctggggtact caaatgtaga atttctgaga ggagacattg
      901 aagagatgcc actacctgac aacagctatg atgtagtgct ttcaaactgt gtactcaatc
      961 tggttcctga taaacagaag gcgttcagtg agattttcag ggttttaaag agtggcggac
     1021 atttcagcgt ctcagatgtg gtggtaaagg gaaatctgtc agaaagggcg cgatccgatg
     1081 cagagttgta tgccggatgt gtttccggag cttcaaccat gaatcagtat ctcacgttta
     1141 taaagaaagc cggatttaat gacataaact tccacaagca aaagaggatt gagttgccgg
     1201 agggcattat tgaccccacg gaagatcagg acgacaatgg agtatacagc attaccatat
     1261 cagccatcaa gcccggaatc atcagctgat cagcagccaa aaacttgtgc agtactttat
     1321 aaaattgcaa atcgcatatc gcaaatctca aattctgaat atgccttcaa aaaaacgact
     1381 caaactgctt gaccggtatc tcaccctgtg gatcttcctg gctatggcgg ccggagtgat
     1441 tatcggatgg ctgagccctg agatcgccgg attctggaac aatctttcca gtggaacgac
     1501 caatatacca attgccattg gcctgatagt gatgatgtat ccgcctctgg ccaaggtgaa
     1561 atatgaagaa ttagcggatg tgttcaggaa cacccgcgtg ctcggtctgt cacttgtgca
     1621 gaactggata atcggtcccg ttctgatgtt cctactggcc atactgttcc tgaggttcaa
     1681 catcgattat atgtatggtc tgatacttat cggcctggcg cggtgcatag ccatggtgat
     1741 tgtctggaat gaccttgcaa aaggggatac tcagtacgca gctggactgg ttgctttcaa
     1801 ctccatcttc caggtgctct ttttctcagt gtatgcatac ttttttattg ccgtcctgcc
     1861 cggctggttc ggcttaccgg tgaatgatgc tgttgaacag atcacaatcg gtcagattgc
     1921 caaaagtgtc ttcatctacc ttggtatacc cttcattgca ggattcctga cacgcttcat
     1981 ccttataagg gtgaagaata aggagtggta tcatacaaag ttcatcccga agatcagccc
     2041 gctcaccctg attgctttgc tctttacaat tttcgtaatg ttttcgctga aaggcgaata
     2101 tatcgtgagg ataccacttg atgtggtacg gatagctatc ccgctggtta tctattttgt
     2161 cataatgttc tttgtctcct tcttcatgag caggagagcg ggggcgaact atgaacagac
     2221 tgcaacactg tctttcacgg cagcaagcaa caacttcgaa cttgcgatag ccgtagcaat
     2281 tgctgttttc ggtattaatt ccggcgaagc ctttgctgcg gtgataggac ctctgataga
     2341 ggtgccggtg atgattgcac tggtgaacgc cgctctgagg ttgaaaacga agtatttcag
     2401 gcaggcagag gcctgacggc agggcggact gacataactg agactttcct ccttaggaga
     2461 tcagagtcgt aagatgcagg cattggtgct gcatcggccc ggttaatagt tcttccggta
     2521 aggatactcg aattacttcc tgcgaaagat tcgcggaatg aatttcggaa cctccttctg
     2581 gtaggttgaa tattccgggt acttgctgct gcttatgttc tcggtcatga tggaactgcc
     2641 gatgaagagc agcaccagca aaacaggtcc gagcagtgtg aagttgatcc atttgccgga
     2701 ggcagctact ccgaagaaat aaaagctgat ccagattgcc tgctcggagg cgaaattcgg
     2761 atgacgcaaa tatttccata agccctcagt caggaaaccc ttattcagcg agtcgcggta
     2821 ctcgtgttcc ccgcctgagg cagatcgctt cattgtctgg aacctgtact gctggttgtc
     2881 ggcaacagtc tcagttatca gaaaggtgat catcagcgct gcagccagaa tgtcagttgc
     2941 acccagggga ctctcaggat gaagtgctgc catgaggatt ggtgtcgtga agagcaggat
     3001 gacaacattc tggtaaagtg agatgaataa aaggttgaac gccccgaacc tgagcctgcc
     3061 tttaagtgcg gagcttttcc tgagaatgct ccagcggtaa tcctcctcac ctttccatgg
     3121 aatgatattg tatcctccct tgcggtggaa gttataggta agccgaagtc cccagagtgt
     3181 aaccagcaca gccatgataa ccgttctggc ggtgtggaat gagcccactg tcacccatgc
     3241 ataggccggc ggcagaaggc tccagagctt gtctacctgg ctgtagttgc gggttatctc
     3301 acttacaatg aaacaggtaa gcactacaat aatcaatatg gtaatccata aattaaccag
     3361 actcagaaac cccgggtcaa aacctgaaag tgaattcaga atattcggaa atactgaaag
     3421 aatgttcacg gcagttcatg ttttcttctc aaatatggga ataatcactc tggtttcatc
     3481 aagcatttat ttttttttaa ctttattgaa tctactaacc aagtgttatt ctagttatga
     3541 cctgtttcaa ttgtcagtgt agcatagctg tcaggttgat ggttattc
//
LOCUS       DAOPXU010000177         3502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_35978_length_3502_cov_14.528866, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000177 DAOPXU010000000
VERSION     DAOPXU010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35978_length_3502_cov_14.528866"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PKK03_12015"
     CDS             <1..872
                     /locus_tag="PKK03_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85166.1"
                     /translation="HKKGAPIVMIVSTIILTLGLIALVVLYYNQKSKMVEMEQVLTEE
                     KDSLANELRMMMYGYDTLKTTNDTLNAEIQRERTRIQKLLEVNASNAQLIRKYKAEIS
                     TMRDIMKSYIVQIDSLNTRNQILTAENVEIKQQMTAVQETNVELEKVKEELTSKVTVA
                     SVIQAKDVVAVALNKKRKETDELRNLDKVRVCFTLRENPIAAAGNKVVYLRVVRPDQL
                     VITPSPDNLFEVKGEQLIFSANRAVDYANSDIEMCIFLDNTGDFIPGTYNVELYLDGE
                     KIGTGAFTLKGRG"
     gene            complement(873..>1688)
                     /locus_tag="PKK03_12020"
     CDS             complement(873..>1688)
                     /locus_tag="PKK03_12020"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HNX85167.1"
                     /translation="LKKAGINRISLGVQSWDDKRLRYLGRRHDAAQSARALDMVFREG
                     IKNVSVDIIYGVPGMTTADLKADLEKTFAFPVTHLSAYHLTVEEGTRFGRMKKEGKLK
                     ETDEETSASMFSLLGKVCREHGFIHYEISNFAKEGYISRHNSSYWKQVPYLGLGPSAH
                     SFDGRSRQWNVADVRKYVKAISSGEIPCEREELDRITVFNEYVMTSLRTMWGIDLTHV
                     EEFYDKELHDYLVNLSGKYIRYGLMKREKNTLVLTDQGRMISDNIIAELLADL"
     assembly_gap    1689..1788
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2024..2329
                     /locus_tag="PKK03_12025"
     CDS             2024..2329
                     /locus_tag="PKK03_12025"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNX85168.1"
                     /translation="MLNIPYIDKMGHFAMYAFFSAVLLLDSCRWRTSRHFRYAILLIP
                     LLFGALMEILQMTLTTSRKAESLDLAANIGGVTAGILLAHIALKILERFRSSQPDHS"
     gene            complement(2301..2741)
                     /locus_tag="PKK03_12030"
     CDS             complement(2301..2741)
                     /locus_tag="PKK03_12030"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNX85169.1"
                     /translation="MLSATCKYGIRAVIFIASKSDTKANTGLKEIAEKLKIPQPYLAK
                     ILQVLARKKILHSAKGPHGGFYLLIPAEKLTLMDIIDAIDGRDFFDSCYVTGERCNLD
                     RPDDGRCILHNDLREEKVRLEKFFSSKTVNQLVAQVRNDPAVKI"
     gene            complement(2742..3182)
                     /gene="rpiB"
                     /locus_tag="PKK03_12035"
     CDS             complement(2742..3182)
                     /gene="rpiB"
                     /locus_tag="PKK03_12035"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNX85170.1"
                     /translation="MEKIIIASDHAGFRLKEKLVSWLISSRYEVRDIGCFSEDSVDYP
                     DYAHPLATAVEKGDYNYGITICGSGNGINMACNKHQKIRSAVCWNDEISRLARSHNDA
                     NVCALPGRFLTEAEAIHIVKIFLTTSFEGGRHKRRIDKIPIKSR"
BASE COUNT          921 a          822 c          746 g          913 t
ORIGIN      
        1 agcataagaa aggcgctccg attgtcatga ttgtgtcaac cataatcctg accctgggct
       61 taattgccct ggtggtcctg tactacaacc agaagagtaa aatggttgag atggagcagg
      121 tgctcacaga agagaaagac tccctggcaa atgagctccg catgatgatg tacggctatg
      181 atacccttaa gacaaccaat gacacgctga acgccgagat ccagcgtgag cgcacaagga
      241 ttcagaaact cctcgaggtc aacgcctcaa atgcccagct gataaggaaa tacaaggcag
      301 agatcagcac catgcgtgat ataatgaaga gttatatagt tcagatagat tcactgaaca
      361 cccgcaacca gatcctcacg gcagaaaacg ttgagatcaa acaacagatg actgcggtgc
      421 aggaaaccaa tgtcgaactt gaaaaggtga aggaggaact gacatccaaa gtcacggttg
      481 cctcggtgat ccaggcaaag gacgtggttg cggtggccct caataagaaa cggaaggaga
      541 ctgatgagct gagaaacctc gacaaggtgc gggtctgctt caccctgagg gaaaacccga
      601 ttgctgcagc aggcaacaag gtggtttacc tgagagtggt gcgccctgac cagctggtaa
      661 tcaccccctc acctgacaat ctcttcgaag tcaagggcga acagctgatc ttctcggcca
      721 acagggctgt tgattatgca aactctgaca tcgagatgtg cattttcctc gacaatacgg
      781 gcgactttat cccgggaacc tacaatgttg aactgtatct tgacggcgaa aagatcggaa
      841 ccggcgcctt cacactgaag ggaagaggct gatcacagat ctgccagcaa ttcggcaata
      901 atattgtcag atatcatcct gccctggtct gtaaggacca gggtattttt ttcccttttc
      961 atcaggccat acctgatata cttgcctgag agattgacca ggtaatcatg caactccttg
     1021 tcgtagaact cctccacatg ggtcagatca atgccccaca tcgttctcag cgatgtcatt
     1081 acatactcat tgaacaccgt tatcctgtca agctcctccc tctcgcaggg gatttccccg
     1141 ctgctgatgg ccttaacgta tttccgcaca tccgcgacgt tccactggcg tgatcgcccg
     1201 tcaaacgagt gcgccgaagg acccaggccc agatagggta cctgcttcca gtacgaagag
     1261 ttgtgccttg aaatatatcc ctccttcgca aaattggaga tctcgtaatg aatgaagccg
     1321 tgctccctgc agactttacc aagcagagaa aacatagatg cagaagtctc ctcgtctgtc
     1381 tccttcagct tcccctcctt cttcatccgg ccaaacctgg tgccctcctc cacggtaaga
     1441 tgataagccg acagatgggt cactggaaat gcaaaagtct tttccaggtc tgctttcaga
     1501 tcagcggttg tcatcccggg gacgccgtat ataatgtcaa cagaaacatt tttaattcct
     1561 tccctgaaaa ccatatcaag agcccttgcc gactgagctg catcatgccg gcggcccagg
     1621 tagcgaaggc gtttgtcatc ccatgactgc acaccaaggc ttatcctgtt tatgccggct
     1681 ttcttcagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa atcacagtaa
     1801 ctgcagaagc tcttgcagaa tggtatgtgt atataaatgc cggccatcgg gaaaatatat
     1861 actttaaaag tacaaaagaa ttaatattag tttgttattt tcaccatctc aaaaaccagg
     1921 catgatatat ctctttataa aacaatatta taagagtata ataatagggt tgttaattct
     1981 gtggctgagt ctttccggaa gcaagtcgat ggtaccgggg cggatgttaa acatacctta
     2041 tattgacaaa atggggcatt ttgcgatgta cgcgtttttc tcagcggtac ttctgcttga
     2101 ttcatgccgc tggcgtacaa gccgtcactt caggtatgca atattactga tacccctgct
     2161 tttcggggct ctgatggaga tacttcagat gacgctcaca acatcgcgca aggccgaatc
     2221 cctggatctt gcagccaaca taggaggtgt cacggcagga atactgctgg cacatatagc
     2281 acttaaaatt cttgagagat tcagatcttc acagccggat cattcctgac ctgggcaacc
     2341 agctgattga ctgtttttga actgaagaac ttttcaaggc gcactttctc ttccctgaga
     2401 tcattgtgaa gtatgcacct gccatcatcg ggcctgtcaa ggttgcacct ctcccctgtc
     2461 acatagcaac tgtcaaaaaa gtcccttccg tcaatggcat caataatatc catcagggtc
     2521 agcttctcag ccggaatcag aagataaaac cctccatggg gtccctttgc tgaatgaaga
     2581 atctttttgc gggcaagcac ctgcaggatc ttcgcaaggt aaggctgggg aattttcagc
     2641 ttttcagcta tctccttaag accggtattg gcctttgtgt ctgatttgga ggcaatgaat
     2701 atcactgccc tgatgccgta tttacaggtg gctgacagca tttacctgga tttgattgga
     2761 attttgtcta tgcgtctctt gtgcctgcca ccctcaaaag aggtggtaag aaatattttt
     2821 acaatatgta tggcttctgc ctcggtgaga aatcttcccg ggagggcaca tacattggca
     2881 tcattgtgtg atcttgccag cctgcttatt tcatcattcc agcaaactgc tgaccggatc
     2941 ttctgatgct tgttgcaggc catgtttatt ccgttgccgg acccgcaaat ggttatgcca
     3001 taattataat cacccttctc aaccgcagtg gcaagcggat gggcataatc cggataatca
     3061 acactgtcct ctgaaaaaca accaatatcc ctgacttcgt acctgctgga gatgagccaa
     3121 ctcacaagct tctccttcag cctgaaaccg gcgtggtctg aggcaattat aattttttcc
     3181 aaaatgacct tatattttta tacaaatata accctgtaat tattaaaaga aaagaaaaca
     3241 tggggagttt gatctgttca taacatgtaa catgaacttt gattatttgt tcatttatta
     3301 aatcatgatc cgggttatca acgccatgtc ttttttcaat taattcaaaa tttttttctt
     3361 accgtttggc gaattacttt caacgcttac tgaacagtgc ccgatcctga tttatcctgg
     3421 acggtcatat taacaaaaca ggtctaacaa ttgttaattg tatttctaat tgtctctttt
     3481 tcagaaaagg tacgtttttt ga
//
LOCUS       DAOPXU010000178         3444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_36912_length_3444_cov_10.350251, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000178 DAOPXU010000000
VERSION     DAOPXU010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36912_length_3444_cov_10.350251"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="PKK03_12040"
     CDS             <1..1434
                     /locus_tag="PKK03_12040"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNX85171.1"
                     /translation="LALFAMYLVWLLNTTDFHEAPAELRLKLPLLFFPVVAGSTERTD
                     RSLLRMVMLSFIAGCLFATFAGFAALAGLMPGEIRSSRELALFVPSIRLSILLCFATF
                     SSLWLIFSDGGEERQAGTVGRLYKVMLAAAAAAMSYFLFRLLSVTGLVIFVLLLFLTG
                     IFLITAGRRVRSGIIFLAAAAAATAALTILSLSAWNRLHNPADPSVNGKRQLTLSGRA
                     YTHYPEETLMENGYLVWVNVCEEELRQEWNRRSLKAYDGADAAGNELRVTLVRYITAK
                     GMPKDSAAVATLSDSDIFNIEQGYANPLYARKGSPMARAYEIAWEIDRAAGGANPSGH
                     SLTQRPEFYRAAAGIISKHPWTGVGTGDLARAFSDEYASISSPLKPEYRLRAHNQYLT
                     FGVTFGIPGMILAVALLLIPWLMSGGRFSYLFLVFMTVILISMFNDDTFSSFTGAAFF
                     SYFYTLLIVTSAGKGNIHHPRQRKDEC"
     gene            1453..1932
                     /locus_tag="PKK03_12045"
     CDS             1453..1932
                     /locus_tag="PKK03_12045"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNX85172.1"
                     /translation="MSLNSKDRKFLELAIKTAGENMEDGGGPFGAIITKGENILARAG
                     NRVVPAHDPTAHAEVMAIRMAAEKLGTHDLADCTIYASCEPCPMCLGAIYWAGIRRLV
                     YASDRYSAASAGFSDNHIYEELALDNGSRNIEMVRGLKKEGDSILKRWVELPDKIQY"
     gene            1942..2466
                     /locus_tag="PKK03_12050"
     CDS             1942..2466
                     /locus_tag="PKK03_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNX85173.1"
                     /translation="MGKVYRDFYLADAVTVARQLPGMELIMASGEKYLRYVITETEAY
                     LGDGDRACHASRGRTRRTEPMYLEGGHLYIYFVYGMHWMLNVVTGPADHPQAVLIRGV
                     SQYQGPGRVTKGLGVDGSFNREDLASSGRIWIEDTGFRPALIAGPRVGIDYAGEPWIS
                     KPWRFIIKNEKEKI"
     gene            2463..>3444
                     /locus_tag="PKK03_12055"
     CDS             2463..>3444
                     /locus_tag="PKK03_12055"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNX85174.1"
                     /translation="MKNGFRLWNNITGWAVFAVSLVIYLLTIEPTVSFWDCGEFILCS
                     YRLEVGHPPGAPFFMLLGRFFTLFAGGDTSKVAMMVNILSAFASAFTILFLFWTITRL
                     VRLATGDDETVTGGKSLATLAAGAIGALAYAFSDTFWFSAVEGEVYGTSSLFTAAVVW
                     AMLRWYDEADDPRSGRWIILIAYLMGLSIGVHLLNLLALPVIVLLWYFRKHKPSSRGF
                     IYALLAGFLILGVLNFVFVPGVPKVAGWFELFFVNTLGLPYNTGLYIYLIILTGLIAG
                     GIWYSLKKGRRILNFVMTVIAVLVLGYSSFALIIIRANAHPPMNQNDPSDV"
BASE COUNT          751 a          912 c          963 g          818 t
ORIGIN      
        1 cttgcactct tcgcgatgta cctggtgtgg ctgctgaata caaccgactt ccatgaagca
       61 cctgctgagc tcaggcttaa gcttccgctg cttttcttcc cagttgtggc cggctcaacc
      121 gaaaggacag acaggtcact tctccggatg gtcatgctct ccttcatcgc cggatgcctg
      181 ttcgccactt ttgccggttt cgccgcactt gccggcctga tgcccggaga gatacgttct
      241 tcgcgcgaac ttgctttgtt tgtgccgtca atacgactct ccatactgct ctgcttcgcg
      301 accttttcct ccctgtggct tattttttct gatggagggg aagagagaca ggccggtacc
      361 gtcggaagat tgtacaaggt gatgcttgcc gccgcggccg cagccatgag ctattttctg
      421 ttcaggctcc tctcggttac agggcttgtc atatttgtgc tgctgctctt cctgacgggg
      481 attttcctga tcaccgcggg ccggcgcgtg cgttcgggca taatcttcct ggctgcggcc
      541 gcggcggcaa ccgctgcatt gacaatcctg tcactcagcg cctggaacag gctccataat
      601 cctgccgatc catcggtgaa cgggaagcgg cagctcacat tgtcgggaag ggcttatacc
      661 cactatcctg aggagaccct gatggagaac ggctacctgg tctgggtaaa cgtgtgcgag
      721 gaggaactga ggcaggagtg gaaccggagg agcctgaaag catatgacgg tgccgatgcc
      781 gcaggcaatg agctgcgcgt gactctggtg agatacatca cagctaaggg aatgcccaag
      841 gactcggcag cagttgcaac gctttcagac agtgacattt tcaatattga acaggggtat
      901 gccaatcctc tttatgcgag gaaggggagc cccatggcca gggcatatga gattgcgtgg
      961 gaaattgacc gtgcggccgg cggtgccaat ccctcaggcc attcactcac acagcggcct
     1021 gaattctacc gggcagcggc cggaataatc agcaaacacc cctggacagg agtggggaca
     1081 ggtgatctgg cccgtgcctt cagtgatgag tatgcatcaa tctcctcccc gttaaagcct
     1141 gagtaccgtc tgagggcgca taaccagtat ctgacattcg gtgttacctt cggcattccc
     1201 gggatgatac tggccgtggc cctgctgctt atcccatggc tgatgagcgg tggccgcttc
     1261 tcatacctgt ttctggtctt catgacagtc atcctgatct caatgtttaa tgatgataca
     1321 ttcagttctt ttaccggagc ggcattcttt tcttattttt acaccctgtt aattgtcact
     1381 tcagccggaa agggaaatat tcatcatccc cggcagcgaa aggatgaatg ttaattgtca
     1441 catttaaggg taatgagtct caacagtaag gacaggaaat tccttgaact tgcaataaaa
     1501 actgcagggg agaacatgga agatggcggc gggccattcg gtgcaataat cacaaaaggc
     1561 gaaaacatcc ttgcccgggc aggcaacagg gttgttcccg cacatgatcc gacagcccat
     1621 gccgaggtga tggccatcag gatggcagca gagaagctgg gcacgcatga cctggctgac
     1681 tgcaccattt atgcttcatg cgaaccctgt cccatgtgcc tgggggccat ttactgggca
     1741 ggtatcaggc gcctggtata tgcctccgac aggtacagcg ccgcctccgc cggattcagt
     1801 gacaatcata tttatgaaga gctggcgctt gacaatggca gcagaaacat cgagatggtc
     1861 cgcggactga agaaggaggg agacagcata ttgaaaaggt gggtggagct ccctgacaag
     1921 atacagtact gaacagcagc aatgggcaaa gtctacaggg atttctatct tgcagatgca
     1981 gttaccgtcg ccagacagtt accgggcatg gaactgatca tggcatccgg agagaaatat
     2041 ctcaggtatg ttattacaga gacggaggcc tacctgggtg acggtgacag ggcatgccat
     2101 gccagcaggg gaagaacccg gaggacagaa ccgatgtacc tcgagggagg gcatctctac
     2161 atatatttcg tctacgggat gcactggatg ctgaatgtgg ttaccggtcc cgctgaccat
     2221 ccccaggctg tgctcataag gggggtgtca caataccagg gacccggacg cgtcacaaag
     2281 ggactgggag ttgacggttc attcaacagg gaggacctgg catcgtcagg caggatctgg
     2341 atagaggaca ccggcttccg cccggcgctg atcgcagggc cgagggtggg aatagactat
     2401 gccggtgagc catggataag caaaccatgg cgcttcatta taaagaacga aaaagaaaag
     2461 atatgaaaaa cggattcaga ctgtggaaca acataacggg ctgggcagtg tttgccgtct
     2521 cgcttgtcat ctacctgctg accatcgaac ccactgtcag cttctgggac tgcggggagt
     2581 ttatcctctg ttcctacagg ctcgaggtgg gccatccgcc cggagccccg ttcttcatgc
     2641 tcctggggcg tttcttcacc ctcttcgccg gaggcgatac ctcgaaggtg gccatgatgg
     2701 taaacatact ttccgctttt gcgagtgcct ttacaatcct gttcctgttc tggaccatca
     2761 ccaggcttgt ccgccttgcc accggtgatg acgaaaccgt aaccggagga aaatccctgg
     2821 caactctggc tgccggagcc attggcgcac tggcatacgc tttttcggat accttctggt
     2881 tctcggcggt tgagggtgaa gtctatggca ccagctccct gttcactgct gcagtggtat
     2941 gggcaatgct ccgctggtac gatgaagccg acgatccccg ctcagggaga tggatcattc
     3001 tgatagccta tctcatgggc ctttcaatcg gggttcacct gctgaacctg cttgcccttc
     3061 cggtgatagt cctgctctgg tacttcagga agcacaagcc atcttcccgt ggcttcatct
     3121 atgcacttct tgccggtttc cttatcctgg gtgtgctcaa cttcgtgttc gtgccgggtg
     3181 tgccaaaggt ggccgggtgg tttgaacttt tctttgtaaa caccctgggc ctgccttaca
     3241 atacgggcct ttatatctat ctgatcatcc tcaccggcct gatagcggga gggatctggt
     3301 attcactgaa gaaagggcgc aggatactga acttcgtgat gacggtgata gccgtccttg
     3361 tgcttggtta ctcatcgttc gccctgatta tcataagggc caacgcccac ccgcccatga
     3421 accagaatga cccgtcagat gtct
//
LOCUS       DAOPXU010000179         3403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_37545_length_3403_cov_12.951613, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000179 DAOPXU010000000
VERSION     DAOPXU010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37545_length_3403_cov_12.951613"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PKK03_12060"
     CDS             <1..320
                     /locus_tag="PKK03_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85175.1"
                     /translation="AVNKSSVNIELLSFYGGNGIVQELVEHGYIISGDSSGFVFTNRR
                     YIYLGGTLSGNSFICVFPFAIEFFRKNHLPIYDTTLIYCGAKLRTPAKSEIMQLKDLK
                     YKK"
     gene            752..>3403
                     /locus_tag="PKK03_12065"
     CDS             752..>3403
                     /locus_tag="PKK03_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85176.1"
                     /translation="MTLNSGIEFRIRSFYPNFFSLLQALDSKGNEGAPSAGHDFRKPE
                     LEYRRPGEASFHELPLTFTDVLVLNQRDRQSAHRYLDLLLASSGNDLPALKEKNLFRS
                     NYLETLKLRMKQLTFTENMMVVPSCTTNPYDEIMLSHKGKMLLDLYREGYPVPDFCTL
                     TSNSYQLDGPDREACLLDAIANLEKMTGEELGGQDHPLIFAMRSATPYYIPGVMPTYL
                     NVGVTKTSLPGLEKQYGKEVADKIYLNNLRNICQILAQGHDFFIGKQEDEMPSAGDSE
                     ARIGKLFKWIEATDERILYDARYQASVFMNNSHSFFKRNQDLIYTFQRGHLTYPALIL
                     QKMVWTIRNKESYPGVLYSRHPRTGLGMQIESVRGIFGEDIMTGTIHAEQREYFHRQE
                     IKEEFPAIYHFTPILPKLEEKLKSPATIEFAVESFEGTSFFALLQLNMSELTGRSTLL
                     TAIDLYQKKIISKERVINLIHPYHLRQIFSERIDDASLKSMILFGNGVSVLPRSAVSA
                     RIYFTMAKALEAKKRGERVCLCKENFIPSETIFMGELDVILSMNPLAIHVVTACLGYG
                     IPAFIDLRNHGIELTEDFISNEAGMVIQEGEWITVSSKNHSIYAGKASFKPARFQKYL
                     EGKTFEMDIKEETVFINMARAYKAYQEILSTLNTEEAMTLPDLVKLIRNDYDQRPEKA
                     VEFVNGWFDKHSDYYYSEILKSGLGSHLDQHKIYNLLTTDRKITFFRNVIRMCLSDNL
                     SGFTAGAFMLGRFLCQVHPIAFWHAFSPQGIAFMLNEYILFEKYMQVLSEFGERHLNR
                     TRNKILTDDLNTIALSSMDTDAFLTLKLTAPDWDGIQSSMPPFCDKDSLQFIALLKQP
                     YGRLYDYNLAWSLEKLTAVCQTEGIAVP"
BASE COUNT          905 a          936 c          772 g          790 t
ORIGIN      
        1 atgcagttaa taaaagcagc gttaatattg agctattgtc attttacggc gggaacggaa
       61 tcgttcaaga actggtggaa cacggctaca tcatatccgg cgattcatca ggctttgtgt
      121 tcacaaaccg cagatatatc tatcttggag gcacattatc cggaaatagt ttcatttgcg
      181 tattcccgtt cgcgatcgaa tttttcagga aaaatcacct gccgatttac gacacgactc
      241 taatctattg cggtgcaaaa ttgaggacgc ccgctaaatc agaaattatg caactcaaag
      301 accttaaata caaaaaataa tcttctgaag acatccctca tcaaatccgg gtcgttatac
      361 cccgatgatg ccgttgccat aatctctgaa agcccggatt catacgcagg ttgccgcgcc
      421 ggttcaattc ctgcctgccc cgcaggactg caggcagccg ggaatttcac tatcttccgg
      481 caatgtctgt tcacaatttt actttcttgt atagcatatc gttggatagt aaaagacaaa
      541 acaacagtga atagttgaac ggctttaaga cgtacatttg tcagatgccg atcatccgga
      601 taaaaaagct gtttttaagc tgttgcgaag cgatgcaaat cttccggtaa ggaggctgac
      661 gacctgacgg cggagatacc ggttccctga atcaggtctc tttcactgca catctgttga
      721 actgaatttc cggccagaaa aacattcagc tatgacgtta aactcaggta tcgaattcag
      781 gataaggtct ttctatccga actttttctc ccttctgcag gcgctggaca gcaaaggtaa
      841 tgagggcgcc ccctccgccg ggcatgattt caggaaaccc gagctggagt acaggaggcc
      901 gggcgaagcg tcgtttcatg aacttccgct gaccttcacc gatgtgctgg tcctgaatca
      961 gcgagaccgg caaagcgccc accgctacct tgacctttta ttagcctcat ccgggaatga
     1021 cctcccggcc ctgaaagaga agaatctctt tcgcagcaat tacctggaga cgctgaagct
     1081 gagaatgaag caactgacct tcacggagaa catgatggtt gttccgtcct gcaccacaaa
     1141 tccctatgat gagatcatgc tcagccacaa aggcaaaatg ctgctcgacc tctatcgcga
     1201 aggatacccg gtccccgatt tctgcaccct cacttcaaac tcttatcagc ttgacggccc
     1261 cgaccgggaa gcctgccttc tcgacgccat agccaacctg gaaaaaatga ccggcgagga
     1321 acttggcggg caggaccatc ccctcatctt tgcaatgcgc tccgctacgc cctactacat
     1381 acccggagtg atgcccacct acctgaatgt cggggtgaca aaaacgagcc tgcccggcct
     1441 tgaaaagcag tacggtaaag aagttgccga caaaatctat cttaacaatc tccggaacat
     1501 ttgccagata ctcgcgcagg gacacgactt tttcatcggg aaacaggagg acgagatgcc
     1561 ttctgccgga gattccgaag ctcgcatcgg aaagcttttt aaatggattg aagccactga
     1621 cgaaagaatt ctttatgatg cccgctacca ggcttctgtt ttcatgaaca attcccacag
     1681 cttcttcaag cggaaccagg atctcatcta tacatttcag cgcggacatc tgacttaccc
     1741 ggccctgatc ctgcagaaaa tggtatggac catccgcaac aaggagtctt acccgggagt
     1801 tctttacagc cgccaccccc gcaccggact aggcatgcag attgaaagcg tcaggggaat
     1861 tttcggcgag gacatcatga ccggaacgat tcatgctgaa cagcgcgaat attttcaccg
     1921 gcaggaaatc aaggaggaat tccccgccat ctatcacttc acccctatcc tgcccaaact
     1981 tgaggaaaaa ctaaaatcgc ctgcaaccat tgagtttgcc gttgaatcgt tcgaaggcac
     2041 ctctttcttt gctttgctcc agctcaacat gtcggaactg accggccgtt caaccctgct
     2101 caccgccatc gacctgtatc agaaaaaaat catatccaag gaaagagtca tcaacctgat
     2161 acacccgtac cacctgaggc agatattttc cgaaaggatt gacgacgcat ccctgaaatc
     2221 gatgatcctt ttcggcaacg gggtttccgt ccttccgcga tcggctgtat cggccaggat
     2281 ttacttcacg atggcaaaag cgcttgaagc aaaaaaaaga ggagaaaggg tgtgcctatg
     2341 caaagagaac ttcatccctt ccgaaaccat cttcatgggc gaacttgacg tgatcctcag
     2401 catgaacccg ctggctattc acgtggtcac cgcctgcctg ggatacggca tcccggcctt
     2461 catcgacctt cggaaccacg gtattgagct caccgaagat ttcatcagca atgaggccgg
     2521 aatggtcatt caggaaggcg agtggatcac cgtatcgagt aaaaaccaca gcatctacgc
     2581 cgggaaagcc agttttaagc ctgcaaggtt tcagaaatac ctcgagggta aaacttttga
     2641 gatggacatc aaggaggaaa cggtgttcat caacatggcg cgcgcctata aggcgtacca
     2701 ggaaattctc agcacactca acaccgagga agccatgacc ctacccgacc tcgtgaaact
     2761 gatccgcaac gactatgacc agcggcctga aaaagccgtt gaatttgtca acggatggtt
     2821 cgacaagcat tcggattatt attattcaga gattctcaaa agcggcctcg gttcccatct
     2881 ggatcagcat aaaatctaca acctgctgac caccgatcgc aagattacct ttttcaggaa
     2941 tgttatccgc atgtgcctga gcgacaacct gagcggtttt actgccggtg cctttatgct
     3001 aggccggttc ctctgccagg ttcatcccat agccttctgg cacgcctttt ccccgcaagg
     3061 gatcgccttc atgctgaacg aatatatcct gtttgaaaaa tacatgcagg tgctctccga
     3121 gtttggggaa cggcacctga accgcaccag gaacaagata ctcaccgacg acctgaacac
     3181 catcgccctt tcgtcgatgg ataccgacgc ttttctgacc ctgaaactta cagctccgga
     3241 ctgggacggg attcaatcct cgatgccgcc cttctgcgac aaagacagcc tgcagttcat
     3301 cgcccttctg aaacagccct atggccggct ctatgattac aaccttgcct ggtcgctgga
     3361 aaaacttacc gccgtttgcc aaacagaggg cattgccgtc ccc
//
LOCUS       DAOPXU010000180         3320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_38911_length_3320_cov_11.847473, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000180 DAOPXU010000000
VERSION     DAOPXU010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38911_length_3320_cov_11.847473"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PKK03_12070"
     CDS             <1..919
                     /locus_tag="PKK03_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX85177.1"
                     /translation="EMAFIVIGAIGFIWMGFWVFMYKKPHEHPLVNKAELDYINQDTI
                     SETKANNNVARPEKKIPFLQTFKYRQTWAFAFGKFMTDGVWWFYLFWTPAYLSDVYGL
                     TSDTTMAKTLIFVLYAIVMLSIIGGWLPKYFVDKLGMDPYAGRMRAMLIFAFFPLLAL
                     LAQPLGHASYWFPVIIIGIAGAAHQAWSANLFSTIGDMFPKTAIATITGIGGMAGGIG
                     AFIINKGSGALFDFASGTTLVDGQAAVMTKELLAAGAQYLNGPMKFFGFEGIRAGYFI
                     IFCICAVAYLIGWIVMKMLVPKYKPIVID"
     gene            935..1780
                     /locus_tag="PKK03_12075"
     CDS             935..1780
                     /locus_tag="PKK03_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX85178.1"
                     /translation="MNDFSDLRGKVAAITGGSGIIGKTLARGLCNAGVNVIILDYKAE
                     RGAMTAKEITAGQESGLATSFDANVIDRDSLLRARDRIREKFGSLDILINCAGGNKAT
                     ATTAKEYIAGGEKDLQGTFFDLDIEGFDGVFDLNFKGTVLPTMILAEDMVSRGSGVIL
                     NISSMNGYRPLTKIPAYSAAKAAINNLTQWLAVHFAPAGVRVNAIAPGFLLTNQNRFL
                     LTDEKTGGMTPRGKKIISATPMGRYAEPEELVGTMLYLLSGASSFVTGVVIPVDGGFS
                     AYSGV"
     gene            1837..2715
                     /locus_tag="PKK03_12080"
     CDS             1837..2715
                     /locus_tag="PKK03_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85179.1"
                     /translation="MGYLEDFKPEKDFFIGIDSDGCVFDTMEVKHKEFFCPATVKFFG
                     LFAIAKYVRETWDFVNLYSVTRGINRFPALIRVMDLLADRPEVAERGVTLPDMEPLRE
                     WVSRETRLGNPLFREYVKANPHPALQKVLEWTLAINEDIAAWLRDTPPFPHARKSIEK
                     LDVVADTMVVSQTPLEALTREWKEHSIDGFVKAIAGQEQGTKSEHIALAAVGKYPPER
                     MLMIGDAPGDLKAADDNGALFFPVIPGDEDRSWKLFHDEALNRFITGTFRGEYQNRLL
                     GEFRRSLPEVPPWKRK"
     gene            2800..>3320
                     /locus_tag="PKK03_12085"
     CDS             2800..>3320
                     /locus_tag="PKK03_12085"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNX85180.1"
                     /translation="MVYYSETDSDRGLNRDKVRAALESILSNFSGRKRMLILPPDITR
                     AHSGAGVITELLWEQLGERITDILPALGTHMPMEAAELSRMFGGTPHTLFREHRWRTD
                     IEELGRVPASFVSSVTGGRLNFDYPVQVNRLLSSGGHDLIISVGQVVPHEVTGMANYN
                     KNVFVGCGGSEAI"
BASE COUNT          846 a          826 c          918 g          730 t
ORIGIN      
        1 ggaaatggca ttcatagtca tcggtgcaat tggattcatc tggatgggat tctgggtctt
       61 catgtacaaa aaacctcatg aacaccccct ggtaaacaag gcagaacttg actacatcaa
      121 ccaggatacc atctctgaga caaaggcaaa caacaatgta gccagaccgg aaaagaagat
      181 ccccttcctg caaacattca aatatcgtca gacatgggct ttcgcattcg ggaagttcat
      241 gactgacggg gtatggtggt tctacctgtt ctggacgcct gcctacctga gtgacgtcta
      301 tggactgaca tcagacacca ccatggcaaa gacactgata ttcgtgcttt acgcaattgt
      361 catgctctca atcataggtg gctggctgcc taaatacttt gttgacaaac tgggcatgga
      421 tccctatgcc gggcgtatgc gcgccatgct gatctttgct ttcttcccgc tgctggcact
      481 tctggcacag ccgctggggc acgcatccta ctggttcccg gtaataatta taggtattgc
      541 cggagctgcg caccaggcct ggtcagccaa tctcttttcc accatcggtg acatgttccc
      601 gaaaacggct attgccacaa ttaccggaat tggcggaatg gcgggcggta taggcgcctt
      661 catcatcaac aagggctcag gagcactgtt tgacttcgca tctggcacaa cattggttga
      721 cggccaggct gcagtcatga ccaaggaact gcttgccgcc ggagcgcagt atcttaatgg
      781 ccctatgaag tttttcgggt ttgagggcat aagggcaggg tacttcatca tcttctgcat
      841 ctgcgccgtc gcgtatctta taggatggat agtgatgaag atgctggttc cgaaatacaa
      901 accgatagta attgattaga taaaaaatat aataatgaat gatttcagtg atctgagagg
      961 taaagtggcc gccataaccg gtggcagcgg gataatcgga aagacgctcg ccaggggcct
     1021 ctgcaacgcc ggggtgaatg taataatact ggactacaag gctgaaaggg gagcaatgac
     1081 agccaaagag atcaccgccg gacaggaatc cggattggca acaagtttcg atgcgaatgt
     1141 gatcgaccgt gattcactgc tcagagcgcg tgaccggata agggaaaaat tcggcagcct
     1201 tgacatcctc ataaactgcg ccggagggaa caaagccact gccacgacag caaaagagta
     1261 catcgcagga ggcgaaaaag acctgcaagg aaccttcttc gaccttgata tcgaaggatt
     1321 tgacggagtc ttcgacctga acttcaaggg gacagtgctt cccacaatga tcctcgcgga
     1381 ggatatggtc agcagaggca gtggagtgat cctgaatatt tcctcgatga atggctacag
     1441 gcctctcacc aagatcccgg cttattctgc agccaaagct gcaatcaaca acctcaccca
     1501 gtggctggcg gtccatttcg cgcctgcggg agtaagggtc aatgcaatcg ccccgggatt
     1561 cctgctaacg aaccagaacc ggttcctcct tactgacgaa aaaacgggcg gaatgacccc
     1621 gaggggtaag aaaataatct ccgctacacc tatgggacgc tatgccgaac cggaagagct
     1681 ggtaggtaca atgctatacc tcctctccgg ggcctcatca ttcgtgacgg gtgtggtgat
     1741 acctgtcgat ggaggcttca gcgcctacag cggtgtctga cggaacattt tctgccggca
     1801 tttgtattat ttttgaaaaa tttttatcag cacaacatgg ggtacctgga agattttaaa
     1861 ccggaaaagg atttttttat cggtatcgat tccgacggct gcgtctttga caccatggag
     1921 gtcaagcata aggagttctt ctgcccggct accgtgaaat ttttcgggtt gtttgcaatt
     1981 gccaaatacg tacgcgagac atgggatttc gttaatctct actctgtaac ccgtggtata
     2041 aaccgcttcc cggcacttat cagggtcatg gacctgctcg ctgacaggcc ggaagtggca
     2101 gaacggggag tgactcttcc cgatatggaa ccgctcaggg aatgggtaag cagagaaacc
     2161 aggcttggca atcccctgtt cagggagtat gttaaggcta acccccatcc tgccctccaa
     2221 aaggtgctgg aatggaccct ggcgataaac gaagacattg cagcctggtt gcgtgacacc
     2281 cctccttttc cgcatgcacg taaatccatt gagaagcttg acgttgtggc tgatacgatg
     2341 gttgtgtcgc agaccccgct tgaggccctg acgcgggagt ggaaggagca ctcgatagac
     2401 ggtttcgtaa aggcaatcgc cggacaggag caggggacca aaagcgaaca cattgccctg
     2461 gcggccgtgg ggaagtatcc cccggagagg atgctgatga taggtgacgc tccgggtgac
     2521 ctgaaggcgg cagatgataa cggagcccta ttctttccgg tgatacccgg tgatgaggac
     2581 agatcatgga aactgtttca cgatgaggcc cttaacaggt tcatcaccgg aactttcagg
     2641 ggtgaatacc agaacaggct gctgggtgaa ttccgtcggt cactgccgga ggtgccgcca
     2701 tggaagcgta aatgagcgga cgaaacggag ccctgcaaag ggtagatgca ataatggcac
     2761 cacccgcagt agcggcagga gacaaaaaaa tggaatgaca tggtatacta ttctgaaact
     2821 gacagtgaca ggggccttaa cagggataag gtcagggcag ctcttgaatc tatcctcagc
     2881 aacttcagcg gaaggaagcg gatgctgata ctgcctcctg acattaccag ggcgcactcg
     2941 ggcgcggggg tgattaccga actgctctgg gaacagctgg gtgaaaggat cactgacatc
     3001 ctcccggccc ttggcaccca tatgcccatg gaggccgccg agctttcaag gatgttcgga
     3061 ggaaccccac acacactctt cagggagcat cgctggcgaa cagacataga ggaacttggg
     3121 agggtgcctg catcattcgt gtcatcggtg acaggcggac gcctcaactt tgactacccg
     3181 gtgcaggtta accgtctcct gtcatcaggt ggccatgacc tgataatctc cgttggccag
     3241 gtggtgccgc atgaggtgac cggcatggcc aactacaaca agaacgtatt cgtgggctgc
     3301 ggcggcagcg aggctatcaa
//
LOCUS       DAOPXU010000181         3225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_40607_length_3225_cov_14.017035, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000181 DAOPXU010000000
VERSION     DAOPXU010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40607_length_3225_cov_14.017035"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PKK03_12090"
     CDS             complement(<1..353)
                     /locus_tag="PKK03_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006043963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNX85181.1"
                     /translation="MKDYNFSEIEKKWQDYWEKNKTFKTPDDLSNPNKYYILDMYPYP
                     SGAGLHVGHPEGYTATDIVARYKRMKGFNVLHPMGWDAFGLPAEQYAIQTGTHPAVTT
                     NKNCDTFRRQIKELGL"
     gene            496..568
                     /locus_tag="PKK03_12095"
     tRNA            496..568
                     /locus_tag="PKK03_12095"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:529..531,aa:Arg,seq:ccg)
     gene            complement(698..1177)
                     /locus_tag="PKK03_12100"
     CDS             complement(698..1177)
                     /locus_tag="PKK03_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX85182.1"
                     /translation="MARFVKTGKKSVKAISTSSLPDIVFMILFFFMAVTTMRETDVMV
                     TVRIPDATEGKVLEKKSLASFIYIGEPLEAFQALYGNEPKIQLNDSFKELADIQDFIA
                     QEREQLNEADKTKMLVVLKIDRDTKMGVVTDVKQALRRASALKIMYANTNVDKITSF"
     gene            complement(1182..1757)
                     /locus_tag="PKK03_12105"
     CDS             complement(1182..1757)
                     /locus_tag="PKK03_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX85183.1"
                     /translation="MARRQVPEINAGSMADIAFLLLIFFLAATTMDSDTGLMRQLPAI
                     PDPNVPQDVNKINDRNILEVKVNKDNLLLVEGQLTQFNQLREIAHNFILNPNDEPSMP
                     EREVVNIPYFGNVAVTKAVISLQNDIGTKYGVYLAVQNELIAARAKLRDDVARQEFGI
                     PYEKLDEDRKSAVDDYYKVPISEAEPKRIGE"
     gene            complement(1798..2259)
                     /locus_tag="PKK03_12110"
     CDS             complement(1798..2259)
                     /locus_tag="PKK03_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85184.1"
                     /translation="MSEKITRLTSILLWVLMGLTVIFAILFYVGGVVPGTEGTRYQEP
                     KVTNSFIVFAYILLVVTVLTLLFFALRNYVMNPKGLKGVLIALGVVAVLVGISALLAD
                     NTVLNLPHYKGKDNVPRTLFWTDIGLYVAYFLLAGAFLTIIYSVISKYFKK"
     gene            complement(2266..3045)
                     /locus_tag="PKK03_12115"
     CDS             complement(2266..3045)
                     /locus_tag="PKK03_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNX85185.1"
                     /translation="MKRVLVFMAFAALLLIGTAQTSQAQTDQTATTTQQLTEQTQTTT
                     PVEGEAVLHKEIKRLFIEGGAGFMGVILLCLILGLAIAIERIIYLNLATTNTEKLLAK
                     VDKALREEGIDAAKEVCRNTRGPVASIFYQGLDRAHEGVEMAEKSVVSYGGVQVGLLE
                     KNLSWISLFIALAPMLGFLGTVIGMIAAFDAIEQAGTVSATIVAGGIKVALITTVFGL
                     VVAIILQVFYNYLLSKIDGISNQMEDSSITLIDMLAKYNKK"
BASE COUNT          765 a          730 c          852 g          878 t
ORIGIN      
        1 aggcccagtt cctttatctg gcggcggaag gtgtcgcagt ttttgtttgt ggttacagcc
       61 gggtgggttc cggtctggat ggcgtactgt tcggccggca ggccgaaggc atcccatccc
      121 atgggatgga gtacgttgaa gcccttcatg cgtttgtacc gtgccacgat gtcagtggcg
      181 gtgtagccct ctggatggcc cacgtgcagg ccggcgccgg aagggtaggg gtacatgtca
      241 aggatatagt atttatttgg atttgacaga tcgtcaggag ttttgaaggt tttattcttc
      301 tcccagtaat cctgccattt cttttctatt tccgaaaaat tgtaatcctt catgtattgc
      361 tgatggttag cgcttatggg ttgcgaaaat aggaaaactt tggcagaaat cccgtccggg
      421 agcgggtggg gtttgccgat ttggctgatt aagttttgaa aatcggcttc ggcggtatat
      481 ttttgcaccc ggaacggtcc cgtagttcaa tggatagaat gacagattcc ggttctgttg
      541 gttgggcgtt cgagtcgccc cgggatcaca gcaaggcggc gcccctatcg ggcgccgtta
      601 tcgttcatac atgcatatgg ccggatgatg ataatcagaa acggtaacgg caggtctcag
      661 acctgccgct ccaacaaaaa tgattacaat attatatcta aaatgatgtg atcttatcca
      721 cgttcgtgtt ggcatacatg atctttagtg cgctggcgcg ccgcagtgcc tgcttcacat
      781 cggtgaccac acccatcttc gtatcccggt caatcttgag aaccaccagc atcttggtct
      841 tgtcagcctc gttgagctgt tcgcgctcct gcgcgataaa atcctggata tctgccagct
      901 ccttgaagct gtcattaagc tggattttcg gctcattgcc atagagagcc tggaatgcct
      961 cgaggggctc accgatatag atgaagctgg cgagcgattt tttctcaagc acctttcctt
     1021 cggtggcgtc aggtatgcga acggtgacca tcacatcggt ctcgcgcatt gtggtgacgg
     1081 ccatgaagaa gaagaggatc atgaagacga tgtcaggcaa tgatgatgta ctgattgcct
     1141 tgacggattt cttccctgtc tttacgaatc ttgccattct tctactctcc tatccttttg
     1201 ggttcagcct ctgatattgg caccttgtaa tagtcgtcga cagctgactt gcggtcttcg
     1261 tcgagctttt catagggtat cccgaactcc tgtcttgcaa cgtcatcccg aagtttggcc
     1321 cttgctgcta taagttcgtt ctgaactgcc aggtaaacac cgtatttggt gccgatgtca
     1381 ttctgcagtg agataactgc tttggtgaca gccacattgc caaagtaggg gatattgaca
     1441 acctctctct cgggcatgga aggttcatca tttggattga ggatgaaatt atgggcaatc
     1501 tctctcagct ggttgaactg ggtgagctgt ccttccacga gcaaaaggtt gtctttattg
     1561 acctttacct cgaggatgtt cctgtcattg atcttattga catcctgcgg aacgtttgga
     1621 tcagggattg cgggcagctg acgcatcagt cccgtatcag agtccatagt ggtggcggcc
     1681 aggaagaaaa taagaaggag gaacgcaatg tcagccattg agcctgcatt aatttccggt
     1741 acttgccttc ttgccatttg gtcagaaatt tactccgccg cagggcggac agttcagtta
     1801 ctttttaaaa tactttgaaa ttacagagta tatgatggtc aggaaagctc cggccagaag
     1861 gaaataggca acatagaggc ctatatcagt ccagaacagt gtacgtggta cgttatcctt
     1921 acctttgtaa tgaggcaggt tcaacacggt gttgtcagcc agcagtgcag aaatgccgac
     1981 cagcacggca acaactccca gcgcgatgag cacgcccttg agacccttgg gattcatgac
     2041 gtagttgcgc agtgcgaaga agagaagggt aaggactgtg accacgagca gaatatatgc
     2101 gaatacgatg aaagagttgg tcaccttcgg ttcctggtac cttgttcctt cagtacctgg
     2161 tacaacgccg ccgacgtaaa acaagatggc aaagatcact gtaaggccca tgaggaccca
     2221 caggaggatc gatgttagcc tggttatttt ttcagacata tctggttatt ttttattgta
     2281 tttggccagc atatcaatga gggtgatcga tgaatcctcc atctggttag aaattccgtc
     2341 gatttttgaa agcaggtagt tatagaacac ctggagaatg atagccacga cgaggccgaa
     2401 tactgtggtg ataagggcca ccttgatacc gccggccacg atggttgctg agactgttcc
     2461 tgcctgttct atggcgtcaa aggcagcgat catcccgata actgttccga ggaatccgag
     2521 catgggagcc agtgcgatga agagagagat ccatgagagg ttcttttcaa gcaggccgac
     2581 ctgtacgccg ccgtaggaaa cgactgattt ttcagccatt tcaactccct cgtgtgctct
     2641 gtcgaggccc tggtagaaaa tacttgccac cgggccacgg gtattgcggc aaacttcctt
     2701 ggcagcatca ataccctctt cacggagggc tttgtccacc tttgcgagga gcttctcggt
     2761 gttggttgtt gccaggttga ggtagataat tctttcaatt gcgattgcaa gaccgaggat
     2821 aaggcagaga aggataacac ccatgaatcc ggcgcctccc tcgatgaaca gtcgcttgat
     2881 ctccttgtgg aggactgctt ctccttctac cggtgtagta gtctgtgtct gttcggtaag
     2941 ttgttgtgtt gtagttgcag tctggtcggt ctgtgcctgt gatgtctgtg ccgtgccgat
     3001 aagcagcaga gctgcaaatg ccataaatac aagaactctt ttcatcatat cagattatta
     3061 aataggaata ttaggacaaa aataaaaaaa aaaggaaagt atacgacctg tatcgaattt
     3121 aaataaaaat taaggattgg cgagaggaag aataagtgtg gcgggggatt acctgcagtg
     3181 atccctgttc cgggtttcat gcaaagtaca tgaacgcctg cactg
//
LOCUS       DAOPXU010000182         3151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_42014_length_3151_cov_10.236434, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000182 DAOPXU010000000
VERSION     DAOPXU010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42014_length_3151_cov_10.236434"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PKK03_12120"
     CDS             <1..708
                     /locus_tag="PKK03_12120"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNX85186.1"
                     /translation="ISDLMKFSGKKNVQAKSIMILGGSRIGRHIALKLQHDCQVKLID
                     IDMEKCRDLAEALEETLVINGDGRNGDLLAEEGLQHMDAFVAVTGNSETNILACLLAK
                     KIGVMKTIAEVENMEYINLAENSGIDTIINKKIATASRIFRHTMNPNVTQVKMMTGTD
                     AEVIEYNVPENARITGGPLKLIDFPKDAIVGGGIRNYKPYIATGDTLIEANDKVVVFA
                     LPSAFDKLNRFLTGDRH"
     gene            complement(784..1398)
                     /locus_tag="PKK03_12125"
     CDS             complement(784..1398)
                     /locus_tag="PKK03_12125"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNX85187.1"
                     /translation="MLIKIYPENPNPAYVRRVTFVLEQGGVVIYPTDTIYAMGCDIRA
                     SKAIDRIARFKGLNPGNPDMSMIFESMSQLSEYTVIHDNNLFRLLKRNLPGPFTFIVP
                     ANSQIPAMFKHKKKTLGIRIPDNSITMAIVRDLGRPLMTTSIHEDDEIMEYITDPELI
                     HEKYDDFADLVVDGGFGSNEPSTVVDCTGPEPVILRQGMGILEF"
     gene            complement(1455..1554)
                     /gene="ffs"
                     /locus_tag="PKK03_12130"
     ncRNA           complement(1455..1554)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKK03_12130"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1670..2215
                     /locus_tag="PKK03_12135"
     CDS             1670..2215
                     /locus_tag="PKK03_12135"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HNX85188.1"
                     /translation="MEKKRSIWKETLNYGIIYGLITVVFSVLTYMFDLTFKTWIIWPS
                     MLLGIIVLFFLLRSYRDHYNGGYITYGKSVGAGVIISIYAAIITAIYIYVLYAFIDPG
                     LMDKSIAMAEAKMAEKGLPEEAIEKAMEMQAKMMKPWFTSLMGIVNSVFYGLILSLIV
                     SLFVMKKGNPLLDELEEEPKQ"
     gene            2217..>3151
                     /locus_tag="PKK03_12140"
     CDS             2217..>3151
                     /locus_tag="PKK03_12140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX85189.1"
                     /translation="MDLTVVVPAYNEAESLPELAEWIDRVCTGSSIGYEIIIIDDGSS
                     DGTWDAVKRISAANNRVKGVRFRRNYGKAAALQTGFEAASGDVVITMDADLQDSPDEI
                     PGLVKMIREEGYHVVSGWKKKRYDPFVKRITSRLYNYTARQFSGIKLHDFNCGLKAYD
                     GEVVKSIEVYGEMHRYIPMLVREAGFRNIGEKVVQHQARKYGITKYGLDRFLKGYLDM
                     LTISFITRFGKSPMHFFGSLGTLMFIAGFIMAAYLGIRKLVFVYQNMRAPLVTDSPYF
                     YIALAVMIIGSVFFVTGFLAELVNRRSSDRNRYLV"
BASE COUNT          834 a          755 c          804 g          758 t
ORIGIN      
        1 atctctgacc tgatgaagtt ctccgggaag aagaacgttc aggcaaagag catcatgatc
       61 ctcggcggca gccggatcgg caggcatatt gcgctgaagc tgcagcatga ctgccaggtg
      121 aagctgattg atatagatat ggagaaatgc cgtgacctcg ccgaggcgct cgaggagacc
      181 ctcgtcatca atggtgatgg caggaacggt gacctcctgg ccgaagaggg actccagcac
      241 atggatgcct ttgtggccgt caccggtaat tccgaaacaa atatccttgc ctgcctgctg
      301 gcaaaaaaaa ttggtgttat gaagaccatc gccgaggtgg agaatatgga atatatcaac
      361 ctcgcagaaa actcggggat tgacaccatc atcaacaaga agattgccac tgccagccgt
      421 atcttcaggc acaccatgaa ccccaacgtc acccaggtaa agatgatgac agggacagat
      481 gccgaagtga tagaatacaa tgtgcctgag aatgcacgaa tcaccggggg cccgctcaag
      541 ctgattgatt tcccaaagga tgccatcgtt ggcggaggga tacgcaacta caaaccatat
      601 atcgcaacgg gcgataccct gatagaggca aatgacaagg tggtggtatt tgcacttccg
      661 tcagcctttg acaagctgaa caggttctta acaggagacc ggcactagaa ccattgccgg
      721 gtagttatca acctattcgt gccacaggca gataagcagc aggatacctc tcctgccgga
      781 gattcagaac tccagtatac ccatcccctg cctcaggata acaggttccg gacctgtgca
      841 atcgaccacc gttgagggct cattgctgcc aaagccgcca tcaacaacca ggtcggcaaa
      901 gtcatcatat ttttcgtgaa tcagttcagg atcagtgata tactccatta tctcatcatc
      961 ctcatgtata gaggtggtca tcaatggtct tcccaggtca cgcactattg ccattgtgat
     1021 gctgttgtca ggtatgcgga tgcccagcgt cttcttttta tgcttgaaca tggcagggat
     1081 ctggctgttt gcaggcacga tgaaggtaaa ggggcccggc aggttgcgct tgagcagtct
     1141 gaacaggttg ttatcatgaa tgactgtgta ctcagacagc tggctcatcg actcaaaaat
     1201 cattgacatg tcaggattac ccgggttaag acccttgaac cttgctatcc tgtcaatcgc
     1261 ctttgatgcc ctgatgtcac atcccatggc ataaatggtg tcggtggggt aaatgacaac
     1321 cccgccctgc tccaggacga atgtcaccct ccgcacatat gcagggttcg ggttttccgg
     1381 gtatatcttt atcagcatcc cttcagtaaa gtgacggcaa atatataaac ttctgatgag
     1441 aggggtaaaa aaaagaaggg taagctactc acatcgcacc gctacaacct tctacccttg
     1501 ctgccttccg accctggggg agttcaaagg gagctggtcg tatgagactt accccgcaca
     1561 aaaatagtaa tttttccggc cagcgccaaa tgttaccgcc acaatacggt gatttcctat
     1621 tcacgcaaat aaagtatatt tgttttcata atctgaaaaa cgcattaaaa tggaaaagaa
     1681 aaggtcaatc tggaaagaga ctcttaacta cgggatcatc tatggtctca tcacggtagt
     1741 cttctcggtt ttaacctaca tgttcgattt aactttcaag acatggatta tctggccaag
     1801 catgcttctg ggcatcattg tcctcttttt ccttcttcgc tcataccgtg accactacaa
     1861 cggcggctac atcacctacg gcaagtcggt gggagccggc gtcataataa gtatttatgc
     1921 agcaatcata acagcaatat acatatatgt gctctatgcc ttcattgatc cgggtctgat
     1981 ggacaagtcg atagccatgg ccgaggcaaa gatggcagag aagggtcttc ccgaagaggc
     2041 cattgaaaag gcaatggaga tgcaggcaaa aatgatgaag ccgtggttca catctctcat
     2101 gggtatagtc aacagtgtct tttacggttt gatactgtcc ctgattgtat cactgtttgt
     2161 tatgaagaaa gggaatcccc tccttgacga gctggaagaa gaaccgaagc agtagtatgg
     2221 atcttaccgt tgtagttccc gcttataacg aagcggagtc gctgccggaa ctggcagagt
     2281 ggattgacag ggtttgcacc gggtcatcaa tcgggtacga gataatcatt attgacgacg
     2341 gcagctcgga cgggacctgg gatgcggtaa aacgcatctc agccgccaac aaccgggtaa
     2401 agggagtccg tttccgccgc aattacggca aggcggcagc cctccagacc ggtttcgaag
     2461 ctgcctccgg cgatgtggtg attaccatgg atgctgatct tcaggatagt cctgatgaga
     2521 ttcccggact ggtgaagatg atccgcgaag agggttacca tgtggtctcc ggctggaaaa
     2581 agaagagata tgaccctttt gtcaagcgaa tcacttcaag actatacaac tacacggcaa
     2641 ggcaattctc cggaataaag ctgcacgact tcaactgcgg gctgaaggct tatgacggcg
     2701 aggtggtgaa gagcatcgaa gtttacggtg agatgcacag gtatatcccg atgctggtgc
     2761 gcgaggcagg tttcaggaat ataggcgaga aggtggtaca gcaccaggcc cgtaagtacg
     2821 ggatcaccaa gtacggtctt gacaggttcc tgaagggtta tcttgacatg ctgacaataa
     2881 gctttataac gaggttcggc aagagcccga tgcacttttt cggctctctc ggaacgctga
     2941 tgttcattgc cggattcatc atggccgcct accttggcat cagaaagctg gtgtttgtat
     3001 atcagaacat gagggcaccc ctcgttactg acagtcctta cttttacatt gcactggcgg
     3061 taatgataat aggttcagtc ttctttgtca ccggttttct ggcagaactg gtcaacaggc
     3121 gttcatccga ccgtaaccgg tatcttgtga a
//
LOCUS       DAOPXU010000183         3114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_42764_length_3114_cov_10.179144, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000183 DAOPXU010000000
VERSION     DAOPXU010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42764_length_3114_cov_10.179144"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PKK03_12145"
     CDS             <1..495
                     /locus_tag="PKK03_12145"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNX85190.1"
                     /translation="EQVIINLLDSEVDRVVVVVGAHRDEIMKVTRRYDVFHCYNEDYR
                     SGMLSSVKCGIYSLPEGCLAALIMPGDQPMTGPGEINRVISSFAASDKGLVMAAFDGR
                     RGHPLIVDLKYTEEILSLPDGEGLRALAERHPGDVLESDTDDPSVIRDIDTREDYMNE
                     ISKT"
     gene            501..977
                     /locus_tag="PKK03_12150"
     CDS             501..977
                     /locus_tag="PKK03_12150"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNX85191.1"
                     /translation="MEEAIRFKLNGRDTELVTDPNQILLWVLRNNFGLTGTKFGCGIG
                     FCGSCTVLIDNEPVRSCMTAVGDIAGRSVVTIEGMETDGKLHPIQQAFIDHDALQCGF
                     CTPGMILTASGLLTKNPSPSREEIVAGLEENLCRCGAHTRIIDAVQDAAGKLKGGK"
     gene            980..>3114
                     /locus_tag="PKK03_12155"
     CDS             980..>3114
                     /locus_tag="PKK03_12155"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNX85192.1"
                     /translation="MKTISGTDKSGSANSGMKRRSFVKALGGGIVLFFAPWSAIDLMA
                     IPAEQRRSLTKDYNAFLHIAEDGTVSCFTGKIEMGQGVFTSLAQMMAEELNVPLEKVK
                     MVMGDTELCPYDAGTWGSLTTRAFGPAMLAAAAEARTVLIQMASEKLGVQPDRLEVKD
                     GVISEAGNPAASVTYAGLARGKKLERYMDIKPAVEDYRKYTLVGKPLKHSDARDKVTG
                     RAKYTGDLKLPGMLHARIFRPPSHGAKLVSVDLSGAEAVDGVKVLRDGDFIAVVSENR
                     DLADLAVVRINAEYAYDELKVNDLTLFDYINRGEYRSNEVASAGNMSEAPQLCDKLFE
                     HEYHDPYLAHAPVETHTALARWEGDKLTVWASTQSPFGLQDTLVREFGTTIEKVRVIT
                     PFLGGGFGGKASSQQGVEAARLARMADKPVMLAWTREEEFMYDAFHPASVIKVKSGID
                     STGKIMLWDYSIYLAGTRGSEATYDIPNVRITNYREDPTKYPVHPFATGPWRAPNNNS
                     NTFARETQIDIMAAAAGADPLEFRLRNLKDERMTGVLKAVAEKFGYVPGQKSARRGRG
                     IGMACGADAGTWVAEIAEVEVNMTTGDVKVIRIACAQDMGLCVNPQGTLIQMEGCLTM
                     ALGYTFTEEVKFEGGVVRDKNFDSYKIPRFSWVPQLECVIMDKMDQPPQGGGEPAIIA
                     VGAAVGNAIFNATGARLFRVPFTPERVLEA"
BASE COUNT          800 a          725 c          919 g          670 t
ORIGIN      
        1 gagcaggtca tcatcaacct tcttgactct gaagtcgaca gggtggtggt ggttgtcgga
       61 gcccacaggg atgagattat gaaggtaacc cgccggtacg acgtctttca ttgttacaat
      121 gaggattacc ggagcggaat gctctcatca gtgaaatgcg gtatctattc acttccggaa
      181 ggatgccttg cagcactgat catgccgggc gaccagccca tgaccggacc cggagagatc
      241 aacagggtga tcagctcttt cgctgcaagt gacaagggcc tggtaatggc agcattcgat
      301 ggcaggcgcg gccacccgct gattgtcgac ctgaagtaca cagaggagat actttcactg
      361 cctgacggag aaggactgag ggcgttggct gagagacacc cgggtgatgt cctggagtca
      421 gacactgatg acccctcggt aattcgtgat atagatacac gtgaagatta catgaatgaa
      481 ataagcaaaa cctgagaatt atggaagaag caatccggtt caaactgaac gggagggata
      541 cggagctggt gactgatcct aaccagatcc tcctctgggt tctgaggaat aatttcggtc
      601 tgaccggtac caagttcggc tgtggaattg gattctgcgg ttcatgtacg gtactgatag
      661 acaatgagcc agtgagatca tgcatgacag ccgtaggtga tatcgccggc agaagcgtgg
      721 taaccatcga agggatggag actgacggaa aactgcatcc cattcagcag gccttcattg
      781 atcatgatgc cctgcagtgc ggtttctgca ccccggggat gatacttact gcatcaggtc
      841 tcctcaccaa aaatccttct ccctcccggg aggagatagt ggcaggtctc gaggaaaacc
      901 tctgcaggtg cggagcccat accaggatca ttgatgcggt tcaggatgcc gcaggaaagc
      961 taaaaggagg aaagtgaaga tgaaaaccat ttccggaacc gataagagtg gttctgccaa
     1021 cagtggcatg aaacgcagaa gttttgtcaa agccctggga ggaggcatcg tcctcttctt
     1081 cgcaccatgg agtgcaattg acctgatggc cattcctgca gagcagcgca ggagcctcac
     1141 caaagactat aatgccttcc tccatatcgc agaggatggc acggtaagct gtttcaccgg
     1201 caagattgaa atgggtcagg gtgtgttcac atctcttgcc cagatgatgg ccgaggaact
     1261 caatgtgccc ctggagaagg taaagatggt tatgggagat actgagctgt gcccctatga
     1321 tgccggtaca tggggatcac tcaccacacg ggcattcggt ccggcaatgc tggctgccgc
     1381 agctgaagcc aggacagtcc tcatccagat ggcctccgaa aaacttggtg ttcagccaga
     1441 ccgcttggaa gtaaaggacg gtgttatctc agaagctggg aatcctgcag ccagtgtcac
     1501 ctatgcaggg cttgcaagag gaaagaagct tgagagatac atggatatca aaccagctgt
     1561 tgaagattac aggaagtata cacttgtggg caagcctctg aagcacagtg atgcccggga
     1621 taaagtcacc ggcagggcga aatataccgg tgacctgaag ttgccgggaa tgcttcatgc
     1681 caggatattc aggcctccgt cacacggggc gaagctggtc agtgttgacc tctccggggc
     1741 tgaagctgtg gacggagtaa aggtgttgag agacggcgat ttcattgcgg ttgtaagcga
     1801 aaaccgggat ctcgccgatc tggccgtagt gaggatcaat gctgaatatg cttatgacga
     1861 gctgaaagtc aatgatctga ctctgttcga ttacataaac cgcggtgaat accggtcaaa
     1921 cgaggtggcg tcggcgggga acatgtctga agcaccacaa ctgtgtgaca aactttttga
     1981 gcatgaatac catgatcctt atcttgccca tgcccctgtt gaaactcata ctgcccttgc
     2041 ccgctgggag ggggataagc tgacagtatg ggcttccacc cagtcacctt tcggcctgca
     2101 ggatacgctg gtcagggagt ttggaacaac cattgagaaa gtaagagtga ttaccccctt
     2161 cctcggcgga ggtttcggtg gaaaggcttc atcacagcag ggtgttgagg cagcgaggct
     2221 ggcacgcatg gcggataaac cggtaatgct ggcatggaca agggaggagg agtttatgta
     2281 tgatgccttc cacccggcat cagtgataaa ggtgaagtca gggatagaca gcaccggcaa
     2341 gataatgtta tgggactaca gtatctatct tgccggcaca cgcgggtcag aagccaccta
     2401 tgacattccg aacgtaagaa ttacaaatta ccgggaagac ccgacgaagt atcctgttca
     2461 tccgtttgcc accggcccat ggagagctcc caacaacaat tccaatacat tcgccaggga
     2521 gacgcagata gatatcatgg cagcggcagc cggtgcagat cctctcgaat tcaggctgag
     2581 gaacctcaag gatgaaagga tgaccggggt gttgaaggct gttgctgaga aattcgggta
     2641 tgtacccgga cagaagtcag cccgacgtgg aagaggcatc ggtatggcct gtggggcaga
     2701 cgccggcacc tgggttgccg agattgcaga agtggaagta aacatgacca ccggggatgt
     2761 gaaggttatc cggattgcat gtgcacagga catgggtctc tgtgttaacc cgcagggaac
     2821 actgattcag atggagggtt gcctgaccat ggctctcgga tatactttta cagaggaggt
     2881 taagtttgag ggcggcgttg tcagggacaa gaacttcgac agttataaga tcccgagatt
     2941 ttcctgggtt ccacagcttg agtgcgtaat aatggacaaa atggaccagc ctccccaggg
     3001 aggcggtgaa cctgccatta tcgctgttgg tgctgcagtg ggaaatgcca tcttcaatgc
     3061 caccggggca agactgttca gggtgccatt cacgccggaa cgggttctgg aggc
//
LOCUS       DAOPXU010000184         3110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_42844_length_3110_cov_13.914566, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000184 DAOPXU010000000
VERSION     DAOPXU010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42844_length_3110_cov_13.914566"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..646)
                     /locus_tag="PKK03_12160"
     CDS             complement(8..646)
                     /locus_tag="PKK03_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85193.1"
                     /translation="MTNQTDQHLEDIQAIRRLMESSSRFLSLSGMSGIIAGVLGIAGA
                     LGAQLIITRISAPVDWYARPFAEGPDGIREFLPLFVLMAVVLILAFSGAAFLSTRKAR
                     KSGIKAWTPVTRRMLVSLLIPLGTGGLFILLTAGTVPAGVIIASTLIFYGLAVISAGK
                     FTFGEIHWLGVLEVAAGLVCLALPQYSVLIWILGFGLLHIVYGLFMHLRYRG"
     gene            complement(766..1905)
                     /locus_tag="PKK03_12165"
     CDS             complement(766..1905)
                     /locus_tag="PKK03_12165"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HNX85194.1"
                     /translation="MKIPRIIVHPTAMKITTKIVDRTTPKIPRKIASLTTMKITTKIV
                     SLTVVVLAVMIHAGCERVIDYRGPETEPKAVIYALLEPDRLISVTVSESHSVFQVPWQ
                     PRQINDATVRVYRDGDLLETLAYKSPEPADENAPVSPYSLYSSVTNKPEFGHTYRIEV
                     LIPGYPAAYGETELPDPVDVEVADTSSSDIGYGNRQLNVKLRFRDPADEENYYRFTAP
                     ATMGNYYAFPGEPYDPGQKVYIRIDDLSYGALSDPIISPMQDDDIFGIYPQNEYYVFT
                     DELIPGKEYTLNLKYTGFNFNTEHYEFLRASFRLNSITRDLYLYLQSYSVYGQTEGNP
                     LAEPVPVYTNIINGLGIVGALSFSQDSLKIGDYPVEGVQYEMQPY"
     gene            complement(1911..>3110)
                     /locus_tag="PKK03_12170"
     CDS             complement(1911..>3110)
                     /locus_tag="PKK03_12170"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX85195.1"
                     /translation="IEDVGAKIDFDYYLSPKHSVKFGAGYTFHHFKPGVTTFRFDSGI
                     IGDTGLDTVVGDMKVRASEFIMYAEDNIDLTPKLKMNAGVRLSLFNVQDTSYFVFQPR
                     LSLRFMATEDLSFKLSYSKMGQFVHLLTTSAISLPTDLWLPVTRRYEPPVSHQVALGS
                     GFLIPGDLEVTVEAFYKTMDNLIEYKEGAFFGGTGAGWESKVEKGRGWAYGSEFMVEK
                     NYGKLTGWIGYTLSWTQRKFENLNYGNTFPAKYDRRHDVSLALTYKFNDRVDAGLVWV
                     YGTGNAATLGASQYPGGNLFAYDSRYDMITDFPGRNNYRLPSYHRLDLGVNLHKQKKH
                     GVRTWSFSVYNAYNRLNPFFVYWGERWVEEPDPDNPGGMIYYSKPELRKVTVFPIIPS
                     VSYSFKF"
BASE COUNT          763 a          812 c          765 g          770 t
ORIGIN      
        1 atctgtttca tcccctgtac ctgagatgca tgaatagtcc gtagacgata tgcaggagtc
       61 cgaatcccag aatccatatc aacacgctgt actgcggcag tgcaaggcag acaagacctg
      121 cagccacctc aagcactccc agccagtgta tctcgccgaa tgtgaatttt ccggcgctga
      181 taacggcaag tccgtaaaag atcagggtgg acgctataat aacaccggca ggaacagttc
      241 cggctgtcag aagaatgaac aatccgcctg taccgagtgg gatgagaagg cttaccagca
      301 tcctcctggt gactggcgtc catgctttga taccgctttt ccgggctttc cgggttgaaa
      361 ggaaagctgc ccctgaaaat gccagtatca gaaccacagc catcaataca aacaagggga
      421 ggaactcacg gataccatcc ggaccctctg caaacggccg tgcataccag tcaacaggtg
      481 cggaaatcct ggtgatgatg agctgtgcac ccaacgctcc ggcaatgcca agaactcctg
      541 cgataatacc ggacatgccg ctgagcgaca ggaacctgga ggaagactcc attagcctcc
      601 tgatggcctg gatatcttca agatgctgat ctgtctgatt tgtcattgtg ttttgtagta
      661 ctttgcgcta caaagttaac caatgttttt tatccctgca agaaaaagtg agattatttt
      721 tggggggcga taattgagga tgcctgtaag tcttaaacct gaaggtcaat acggctgcat
      781 ttcatactgc accccctcga cagggtaatc ccctatcttc agggaatcct gtgaaaatga
      841 gagcgctccc acaatcccca gtccgttgat aatattggtg tagaccggca ccggttcggc
      901 aaggggattt ccctctgtct ggccatagac cgaatatgac tgaaggtaca gataaaggtc
      961 cctggttatg gagtttaacc tgaaggatgc acggagaaac tcgtaatgct ctgtattaaa
     1021 attaaatccg gtgtacttaa ggttgagggt atactccttt cccggtatca gctcatcagt
     1081 gaagacataa tactcattct gagggtagat gccaaagatg tcatcatcct gcattggtga
     1141 aatgatcgga tcgctgaggg cgccgtagct caggtcatcg atgcggatat aaaccttctg
     1201 tccgggatca taaggttcac cggggaaggc ataatagttc cccatagttg ccggcgccgt
     1261 aaacctgtag tagttttcct cgtcagccgg atcgcgaaag cgcagtttca cgtttaattg
     1321 cctgtttcca taaccgatat cactggagct tgtatcagcc acctccacat ccaccgggtc
     1381 cggcagttct gtctctccgt aagccgcagg gtatcccgga atcaaaacct caatacggta
     1441 ggtatgcccg aattccggtt tatttgtcac agaggagtaa agcgaatagg gactcacagg
     1501 agcgttctca tctgccggtt caggactttt atatgcaagt gtctccagca ggtctccgtc
     1561 gcggtacacc cgaaccgtgg catcattgat ctgccttggc tgccatggca cctggaatac
     1621 agaatgactt tccgaaactg taaccgaaat aagcctgtcc ggttcaagga gggcataaat
     1681 gactgccttt ggctcagtct caggacccct gtagtcaatc accctctcac agccggcatg
     1741 gatcatgact gcaagcacga cgactgtcag ggatactatt ttcgttgtga tcttcatggt
     1801 tgtgagggag gctatcttcc ttgggatttt cggggttgtc cggtcgacta ttttcgttgt
     1861 gatcttcatg gcagtcgggt gaactattat cctcgggatc ttcatggctg tcagaacttg
     1921 aatgaatatg ataccgacgg aattatcgga aagacagtca cctttctcag ctcaggcttg
     1981 ctgtagtaga tcattcctcc cggattgtcc gggtcgggtt cctccaccca tctttcgccc
     2041 cagtaaacaa agaacgggtt gagccggttg taggcattgt acactgagaa gctccaggtg
     2101 cgtactccgt gtttcttctg tttgtgaagg ttcaccccca ggtcgagcct gtggtaagac
     2161 ggtaaacgat agttgttgcg cccgggaaaa tcagtaatca tatcatacct ggaatcatat
     2221 gcaaaaagat tcccgccagg gtactgcgaa gcgccaagcg tggcagcgtt cccggtaccg
     2281 taaacccata ccagtccggc atcaaccctg tcattgaact tataggtcaa tgcgaggctt
     2341 acatcatgcc ttcggtcata cttcgccggg aaagtgttcc cgtagttcag attctcgaat
     2401 ttgcgctggg tccatgacag tgtatatcct atccatcccg tcagctttcc gtagtttttc
     2461 tcaaccatga actctgatcc gtaggcccac cccctgccct tctccacttt cgactcccat
     2521 ccggcacccg tacctccgaa gaaagctccc tccttgtact caataaggtt atccatggtc
     2581 ttgtagaatg cttccaccgt aacctcaagg tctcccggta tcaggaatcc tgaccccagt
     2641 gccacctggt gcgagacagg tggctcatac cgccttgtaa ccggcagcca gagatctgtc
     2701 ggaagactta tggcagaggt ggtaagcagg tgcacaaact gccccatctt actgtatgac
     2761 agcttgaacg acaggtcctc ggtggccatg aaccggagcg aaagccgcgg ctggaagacg
     2821 aaatagcttg tgtcctgaac attgaagagc gacaggcgaa cgccggcgtt catcttcagt
     2881 ttcggggtga ggtcaatgtt gtcctcggca tacatgatga actcactggc ccttaccttc
     2941 atgtctccca caacagtatc aagcccggta tcgccaatta tgcccgaatc aaacctgaaa
     3001 gtggtgacac ctggcttgaa gtgatggaat gtgtaaccgg cgccgaactt cacggaatgt
     3061 ttgggcgaca ggtaatagtc gaaatcaatc ttcgctccca catcctcgat
//
LOCUS       DAOPXU010000185         2921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_47055_length_2921_cov_8.712840, whole genome shotgun sequence.
ACCESSION   DAOPXU010000185 DAOPXU010000000
VERSION     DAOPXU010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47055_length_2921_cov_8.712840"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..1165
                     /locus_tag="PKK03_12175"
     CDS             236..1165
                     /locus_tag="PKK03_12175"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNX85196.1"
                     /translation="MITYWQMIPNGLLPLNSFEKGCIVNVTDPTPSEIGILKTTLKVP
                     EDFLADILDVDERSRMEVEDDMLLMIYRIPYQNQSNGFPFTTIPLGLVLDKNSLVIIC
                     HRNNEVLDDFFSRSGKGITIETKIDLILHLFLRTTMFYHRYLKQINNQTAMIEQDLEK
                     STRNQELHRLLKMEKCLVYFTTSLRSNEMMMLKLKNSKWIRDYEFSSDLLEEATIENR
                     QAIEMAKIYSDIQSGMMDAFASVISNNLNIVMKSLTIVTIILMIPTLIASFYGMNVPN
                     RLEHNMNAFWFILGGSVVLATAGVLLLRRKKWM"
     gene            1191..2636
                     /locus_tag="PKK03_12180"
     CDS             1191..2636
                     /locus_tag="PKK03_12180"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNX85197.1"
                     /translation="MTSINIRSLLKITGLIMIITGGAFIACIPVALIYSESLGPFLWP
                     ALISFVPGLVLYMAMPRTSRDNINVRDGYLNVTLSWVLLALLGTMPYLFSGSVSGVTN
                     ALFETMSGFTTTGATVIGNVEILPKSILFWRSLTHWIGGVGVILLVIVILPNMKAGGY
                     HLFTLESSMKQKILPKTRSIAYIILVIYLAMTVLETVLLRFGDMNLFDSICHSFATVS
                     TAGFSTRNTSISEYSPYVQYVIGIFMFLGGVSYIVYYFLVRREFRKIAAFEELWFYIF
                     FVTGATILVTLILYTGTDRNFALSVRHGFFQVISQMTGTGFATTDYMLWPQGGWFIMI
                     LLMPAGGCTGSTTGGIKMARHLITLRNLKVVFQRFQHHHAVIPIKINDRIVPDNLNIT
                     ILSFTLLYIALTLVGMIAIHFTGVPLIEAVGASTSAISNVGPGLGASGNFGHYNGFTA
                     GAKFIMIALMLIGRLEIFTILALFTRSFWKN"
BASE COUNT          704 a          752 c          716 g          749 t
ORIGIN      
        1 cccacaacaa gtacctttcc catcacgtta tatttaggtt ctgtgcaaag gtaatttttt
       61 tggacatttt ttgcggcccg gggatgcggt tagcataaaa gttatatttt tgcacacgta
      121 tcaatacagt tgtcttttat ggacgattat cacgcgagcc gttttattaa tttagttctg
      181 cagaggtaag ccggaaacgg tacctccgcg gggcggaaaa gtaggaggta ctagaatgat
      241 tacttactgg cagatgatcc cgaacggtct ccttccgttg aactcatttg aaaaaggttg
      301 cattgtaaac gtcactgatc ccactccttc agagataggc atcttgaaga ccaccctgaa
      361 ggtccctgag gatttccttg ctgatattct tgacgttgac gagcgatccc gcatggaggt
      421 ggaggatgac atgctgctga tgatctaccg tatcccctac cagaatcaat caaacggatt
      481 cccgtttaca accatacccc tggggcttgt gctcgacaag aacagcctgg taatcatctg
      541 tcaccggaac aacgaggtgc ttgatgattt cttcagccgc agcggcaagg gcattacaat
      601 agagacaaag atcgatctca tactgcatct ctttctcagg accacgatgt tctaccaccg
      661 gtacctgaag cagatcaaca accagacggc aatgatcgag caggatctgg agaagtcgac
      721 ccggaaccag gaactgcacc gcctgctgaa gatggagaag tgtcttgtat atttcaccac
      781 ctcgctgcga tccaatgaga tgatgatgct caagctgaag aattcgaaat ggatacgtga
      841 ctacgagttt tcatcagacc tcctggagga agctaccatc gagaaccggc aggctattga
      901 gatggcgaag atttacagtg atattcagag cgggatgatg gatgcctttg cctcggtgat
      961 ctcgaacaac ctgaacatcg tgatgaagtc tctcacgata gtcaccatca tattgatgat
     1021 accgaccctc attgccagct tttacgggat gaatgtaccc aacaggctgg agcacaacat
     1081 gaacgcattc tggttcatac tcggtggttc ggtggtgctt gccactgccg gtgtgcttct
     1141 cctgagaaga aaaaaatgga tgtaacagtg tacggtctcg ccgccctgta atgactagca
     1201 taaacatcag atcacttctg aaaattaccg gcctgataat gatcataaca ggtggcgcct
     1261 tcattgcctg cattcctgtt gcccttatct actccgagag cctcggcccg tttctctggc
     1321 cggcacttat atcatttgtg ccggggctcg tgctctacat ggccatgcct cggacatcaa
     1381 gggataatat caatgtcagg gacgggtacc tgaacgtgac actgtcatgg gttttgctgg
     1441 cccttctcgg caccatgccc tatctcttca gcgggtcagt gtcaggggtc acaaatgcgc
     1501 tgtttgaaac catgtcaggc ttcaccacaa ccggggcaac cgtaataggc aatgttgaga
     1561 tactgccgaa gtcaatcctt ttctggcgga gcctgactca ctggatcggt ggcgtgggcg
     1621 tcatcctgct cgttatagtg attcttccca acatgaaggc cggggggtac catctcttca
     1681 ccctcgagtc atcgatgaaa cagaagatac tgccgaagac aaggtcaata gcatacataa
     1741 tactggtcat ctacctggcg atgaccgtac tggagacagt gcttctgagg ttcggagaca
     1801 tgaacctttt tgacagcata tgtcactcct ttgccactgt atccaccgca ggtttctcca
     1861 ccaggaacac cagtatctcg gaatactcac cctatgtcca gtatgtgatc ggaatcttta
     1921 tgttccttgg aggggtcagc tatatcgtat actatttcct tgtacgccga gagttcagga
     1981 agatagctgc ttttgaggag ctgtggtttt acatcttctt tgtcaccggg gccaccatcc
     2041 tggtgacgct gatcctttat accggcactg accgcaattt tgccctctcg gtgaggcatg
     2101 gctttttcca ggtcatctca cagatgacag ggactgggtt tgccacaacg gattacatgc
     2161 tctggccaca gggcggatgg ttcatcatga ttctcctgat gcctgccgga ggatgtacag
     2221 gttcaaccac cggaggaatc aagatggcaa ggcacctgat cacgctgcgt aacctgaagg
     2281 tggtctttca gcggttccag catcaccatg cagtgatacc gattaagatc aacgacagga
     2341 tagtaccaga taacctgaat attaccattc tgtcattcac ccttctgtac attgccctga
     2401 ccctggtcgg gatgattgcc attcatttca ccggggtgcc cctgattgag gccgtggggg
     2461 caagcaccag tgccataagc aacgtggggc cagggcttgg agcttcgggc aacttcggcc
     2521 actacaacgg cttcacggca ggagcaaagt ttattatgat tgccctgatg ctcatcggtc
     2581 gccttgagat tttcaccata ctggccctct tcacaaggtc attctggaaa aactaaacac
     2641 tgccggcgtc tcaaactgcc gaccggtact gcagtcccga tccgcctacc ggccgatacg
     2701 ggattggttc tcctcatttg caggagcccc aaactggtac tgctgactgc ctgctggtaa
     2761 tgcctacttt cccagatgtg ctctgatctt ttccaggttc tgcggcgtgc cgcagtcaaa
     2821 ccagtaaccg tagtcatgga ggcaggtcat gatcacgtgc ttcccagcca gcataaggta
     2881 gagcgatgtc agtgaataaa tgccctcctg catcagttca a
//
LOCUS       DAOPXU010000186         2862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_48571_length_2862_cov_10.600285, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000186 DAOPXU010000000
VERSION     DAOPXU010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48571_length_2862_cov_10.600285"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1781
                     /locus_tag="PKK03_12185"
     CDS             <1..1781
                     /locus_tag="PKK03_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX85198.1"
                     /translation="LAPGDYRFRVISSTNGNIWSNAEASMRIRISPPLWLSLPAYILY
                     LITATLISYYLIITYLKRKAVENQRKIEAIEAGKEREILNAKINFFTNITHEVRTPLT
                     LIKGPLDRILKAGSNNLHDHEENLQIIRKNTDRLLSLTNQLLDFRKTEKEMLKLNFIR
                     TDLYELVESIFNLFLPYSEEKNISVRLNSPVNHYELAIDREATIKILSNLLSNAIKFA
                     GSGVDIFLEPGSEKDAAVRIRVTNDGNLIPGELAEKIFEPFYQLDSNKPGEKGTGLGL
                     SLARSIAELHQGRLYLDTSVTDRNSFVLELPKFQEETISMAEITEGGANPAENEEFEV
                     FSSSAENSPNLLLVEDEVEMGRFIAKEISGEYNVILTHNGEEALNALKKFNIIVVVSD
                     VIMPVMNGYELCRQIKSTMEFSHIPVILLTASVHVNAKIEGLDSGADAYIEKPFATEH
                     LMAQILNLIKNRRREQQNFINSPLAHFRSVALNKTDEDFLKKLNSTLLDNIAETDLSV
                     ERISEIMGISISTLYRKVKALTDLNSVEYIRLTRLKKAAELLAEGNFRINEISYLVGF
                     SSPSYFATSFQKQFGISPSQFARKIK"
     gene            2087..>2862
                     /locus_tag="PKK03_12190"
     CDS             2087..>2862
                     /locus_tag="PKK03_12190"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNX85199.1"
                     /translation="MKKTFVVCLLICMSAGSACFAQSGQASNVFNADTAHVSPYNLSG
                     AAYPRIEADSRVTFRFYAPDAQKVQVSIVNVPFDMVKGEDGFWTYTSEPQDKGFHNYW
                     MVVDGAVVVDPATNAFMGYGHMCNGFEIPDPEGGFYELKDVPHGNVLIKNYYSKVAGS
                     WRHIFVYTPPMYDNTSARYPVLYLQHGGGEDERVWIEMGRTNLILDNLIAEGKIRPFI
                     VVMETSAVGPQFPMFQRPAAAATPAPQAARPAGQAPGAPAA"
BASE COUNT          828 a          659 c          643 g          732 t
ORIGIN      
        1 atctggctcc cggagattac agattcaggg taatttcatc aaccaacggc aatatatgga
       61 gcaatgccga agccagtatg aggatcagga tatctccccc attgtggctc tctttgccag
      121 cttacatttt atacctgatc actgctaccc ttatcagcta ctatctgata atcacctatc
      181 tgaaaagaaa agctgttgaa aatcaaagaa aaatagaggc cattgaggcc gggaaggaaa
      241 gggaaatact caatgccaag atcaatttct ttaccaatat tacccatgaa gtcaggactc
      301 cattgacgct catcaagggt cccctcgaca gaattttaaa ggctggttca aacaacctgc
      361 atgatcatga ggagaatctg cagatcatca ggaagaatac agacaggctt ctgagcctta
      421 ccaaccagtt gcttgatttc aggaagacag agaaagaaat gctgaaacta aatttcatca
      481 gaactgacct gtacgaactt gtggaatcaa tatttaacct gttccttcca tacagtgagg
      541 aaaagaacat atctgtgagg ttgaattcgc cggtgaatca ttacgagctt gccattgaca
      601 gggaagcaac aataaagatt ttgagcaatc tgctctctaa cgcgataaag tttgccggat
      661 ccggagtcga catctttctt gagccaggct cagaaaagga tgctgccgtg agaatacggg
      721 taaccaatga cggcaacctg atccccgggg agttagccga aaagatcttt gaaccttttt
      781 accagcttga ctccaacaaa ccgggtgaaa agggtaccgg tctgggattg tctctcgccc
      841 gctcgatagc tgagcttcac cagggaagac tctatcttga cacttcagta accgatcgca
      901 actcgtttgt tctggagctg ccgaaattcc aggaagagac gatcagcatg gcagaaatta
      961 cagaaggggg cgcaaacccg gctgaaaacg aagaatttga ggtgttcagc tcctctgcag
     1021 aaaacagtcc gaacctgctc cttgtggaag acgaggttga gatgggcaga ttcattgcca
     1081 aagaaatatc aggtgaatac aatgtaattc tgacccataa cggagaagag gcacttaatg
     1141 cattgaagaa gtttaacatc atagtggttg taagtgatgt gattatgccg gtcatgaacg
     1201 gatatgaact atgcaggcag atcaaatcaa ccatggaatt cagccatatt cccgtcatac
     1261 tgcttacagc ctctgttcac gtcaatgcaa agattgaggg ccttgactcc ggcgctgatg
     1321 catatattga gaagccattc gccactgagc atttaatggc tcagatactt aatcttataa
     1381 aaaacagaag gcgggaacag cagaacttca tcaattctcc gctggcacat ttcaggtcgg
     1441 ttgccctgaa caagactgat gaggatttcc ttaaaaaact gaactccacc ctccttgata
     1501 atattgccga aactgatctg agtgtggaga ggatatcaga gataatggga ataagcatct
     1561 caactcttta caggaaggta aaggcgctca ctgacctcaa ctctgttgaa tacatcaggc
     1621 tgacaaggct gaagaaggca gctgaactgc tggccgaagg caatttccgg attaacgaaa
     1681 tatcatacct ggtgggcttc tcttcacctt catattttgc caccagcttc cagaagcaat
     1741 tcggaatttc accctcccag tttgccagga agataaaata aacaacattt tacaagacca
     1801 tcccgattat ggcatgcgcc agtgccatga ccgttttttg cattccactc cactcaatta
     1861 tgctttttta actcttttag ggtcaacttt aaattttcag tcagcatttg cacaaaaaat
     1921 gaacccgttt cagacttttt attaggagtt ttacatcatt acgggctttc aatcccagat
     1981 aatcttgact gatatcaatc aactgaagaa catagtgaaa atgttgtctg aaagcctgcc
     2041 ttcatttctt atacgggaat ttaccaacct aacaggagaa aataaaatga aaaaaacatt
     2101 tgtcgtttgt ctgctgatat gcatgtcagc aggctcagcc tgttttgctc agtcagggca
     2161 ggcatcgaac gttttcaatg ctgacacggc tcacgtctcg ccctacaacc tctccggtgc
     2221 agcgtatcca cgcattgaag cagactcacg ggttacgttc cgcttttatg ctcccgatgc
     2281 acagaaggtg caggtatcca tcgtcaatgt tccttttgac atggtcaaag gggaagacgg
     2341 gttctggacc tataccagtg agccgcaaga caagggattt cacaactact ggatggtagt
     2401 cgacggggcc gtcgtagttg atccggctac gaatgcattc atgggttatg gtcatatgtg
     2461 caacggcttt gaaattcctg atcccgaagg tggattttat gaactgaagg atgtgccaca
     2521 cggaaatgta ttgataaaaa actattattc aaaggtggca gggtcatggc gtcacatctt
     2581 tgtctatact cctcctatgt atgataacac ttcagcccgc tatcctgtcc tttatcttca
     2641 gcatggcggt ggtgaggatg aaagggtctg gattgagatg gggcgcacca acctgattct
     2701 cgacaatctg attgcagaag gaaagatcag accatttatt gttgtcatgg aaacaagcgc
     2761 cgtcgggcct caatttccca tgttccaacg tccggccgct gctgctacac ctgcacccca
     2821 ggcagcacga cctgcaggtc aggctcccgg tgctcctgcc gc
//
LOCUS       DAOPXU010000187         2845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_49042_length_2845_cov_13.307527, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000187 DAOPXU010000000
VERSION     DAOPXU010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49042_length_2845_cov_13.307527"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PKK03_12195"
     CDS             <1..636
                     /locus_tag="PKK03_12195"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3410 domain-containing protein"
                     /protein_id="HNX85200.1"
                     /translation="LLTDCDIITFHVPLTHDGDDKTFHLVDRDILSRLGRQKIIINSS
                     RGEVVDNLALREALKSGRLRGAALDVWEGEPAADPQLIDLADIATPHIAGYSVDGKAN
                     ATISSVRVVADELGLPMHDWAPAELPQPAMPLIDLTGKGGAAPVEMVARAVNHTYPVE
                     EDDLLFRNHRENFEYLRDNYRIRREFPSYRVRTNDPETERILKELGFQITK"
     gene            657..2105
                     /gene="gnd"
                     /locus_tag="PKK03_12200"
     CDS             657..2105
                     /gene="gnd"
                     /locus_tag="PKK03_12200"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HNX85201.1"
                     /translation="MKADIGLIGLAVMGENLVLNMESRGYTVAVFNRTTEKVSAFVNG
                     RGKGKKLIAAFSLEEFISSLSRPRKVMLMVKAGKPVDDFIELLIPLLDKGDIIIEGGN
                     SHFPDTIRRTAYVESKGLLYIGTGVSGGEEGALLGPSMMPGGSREAWPHVKDIFHSIA
                     ARASDGAPCVTWIGDNGAGHFVKMAHNGIEYGDMQLICEAYHLMLSSGAFTYDEMADI
                     FDEWNKGELDSYLIQITGEILRFRDTDGQPLVTRILDSAGQKGTGKWTVISALDHGVP
                     LSLIGESVFARFISSMKKERVKASPLFSNPAGTTIKDKKQFVDDIRKALYASKIVSYA
                     QGFNLLRNAAAEYKWDLNYGEIAMIWRGGCIIRSAFLNKIYEAFRREPGLPNLIMDNY
                     FTGILRENSDSWRRVVAYAVATGIPVPTMSSALAWFDSYRSEWLPANLLQAQRDYFGA
                     HTYQRTDKPEGEFFHTNWTGRGGDTASSTYNA"
     gene            2166..>2845
                     /locus_tag="PKK03_12205"
     CDS             2166..>2845
                     /locus_tag="PKK03_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNX85202.1"
                     /translation="MIKNVNERAADNIRILSMAMVEKANSGHPGGAMGGADFIHILFS
                     EFLRFDPADPEWINRDRFFLDPGHMSPMLYSVLTLTGHFTVDDIMQFRQWGSVTPGHP
                     ELDIKRGVENTSGPLGQGHTMAVGAAIAEKFLTARFGRLFEHRIYAYISDGGIQEEIS
                     QGAGRLAGHLGLSNLVMFYDSNDIQLSTETSAVTSEDTAAKYKAWGWRVMEIDGHSHD
                     QIRGALRMA"
BASE COUNT          721 a          745 c          777 g          602 t
ORIGIN      
        1 ttattgacag attgtgatat cattacattt catgttcccc tcacccacga tggtgatgac
       61 aaaacattcc acctggtaga ccgggatatc ctttcgcggc ttggcaggca gaaaatcatc
      121 atcaattcat cgcgcggcga ggtggtagac aatctcgcgc tgcgtgaagc acttaagtcg
      181 gggagactgc gcggcgccgc ccttgacgtg tgggagggag agccggctgc cgacccgcag
      241 ctgattgacc tggcagacat agccacacct cacattgcag gttactccgt tgacggcaag
      301 gccaacgcca ccattagctc cgtccgggtg gtggccgatg aactcggatt gccaatgcac
      361 gactgggccc cggcggagct gccgcagccg gccatgccgc ttattgacct caccggcaag
      421 ggcggcgccg cacccgtcga aatggtggca cgggcagtaa atcatacata ccccgtggag
      481 gaggatgacc tcctcttcag aaaccacagg gaaaatttcg agtatcttcg cgataactac
      541 agaatcaggc gcgaattccc ctcatacagg gtaagaacca atgacccgga gacggaacgt
      601 attcttaaag aacttggttt tcagataact aaataagata aacaaattat atcaacatga
      661 aagcagacat tggactgata gggctggcgg tgatgggtga gaacctggtg ctgaatatgg
      721 agagcagggg ttatacagtg gcggtcttca accgcacaac agaaaaagta tccgcatttg
      781 tgaacggacg aggcaaggga aagaaactca tcgcggcatt ctctcttgaa gagttcatct
      841 caagcctcag ccggccgaga aaagtaatgc tcatggtaaa ggcaggcaag ccggtggacg
      901 acttcattga actgctgatc ccgctgctcg acaagggtga tataatcata gagggaggta
      961 actcgcattt tcctgacacc atcaggagaa ctgcctatgt tgaaagcaag ggcctccttt
     1021 atatcggtac aggggtttcg ggaggtgaag aaggcgcgct gctcggccct tcgatgatgc
     1081 ccggcggctc cagggaggca tggcctcatg tgaaggatat tttccattcc atcgcggcca
     1141 gggcttctga cggcgctccc tgcgtcacct ggattggcga caacggcgcc ggccacttcg
     1201 tcaagatggc acataacggc atcgaatatg gtgacatgca gctgatatgc gaagcttatc
     1261 acctgatgct cagctccgga gcattcacat atgatgagat ggctgatata ttcgacgagt
     1321 ggaacaaggg tgagcttgac tcctacctga tccagataac cggtgaaata ctccggttca
     1381 gggacaccga cggccagccg ctggtcacca ggatactcga ctcggcaggc cagaagggaa
     1441 caggcaaatg gaccgtcatc agcgccctcg accacggggt gcctctgagc ctcatcggcg
     1501 aatcggtctt cgcccgattc atctcatcaa tgaaaaaaga gagggtaaag gcatctcccc
     1561 tcttcagcaa tccggccggg acgaccatta aggataaaaa gcagtttgtt gatgatatcc
     1621 gcaaggcgct gtatgcatca aagatcgtct cctatgcaca gggtttcaac ctgctcagga
     1681 acgccgcggc agaatacaaa tgggacctca actacggaga gatagcaatg atatggaggg
     1741 gcggttgcat tatacggtca gccttcctca acaagattta cgaagctttc aggcgtgaac
     1801 cgggcctgcc caacctgatc atggataatt acttcacggg gatactcagg gagaacagtg
     1861 acagctggcg ccgcgtggtg gcctatgcgg tggcaacagg catacccgtc cccacaatga
     1921 gctcagccct cgcctggttt gacagctacc gttcagagtg gttgccagcc aacctcctcc
     1981 aggcacagcg tgattatttc ggggcacata cttaccagcg gaccgacaaa ccggaaggcg
     2041 aattcttcca tacaaactgg acgggacgcg gaggtgatac cgcctcttca acctataatg
     2101 cctgaacagg cgccggtaac acttttttct atatttttac cttaaacatt aaggcctgaa
     2161 tcattatgat caaaaatgta aacgaacgcg cagcagacaa tatccgtatc ctttcaatgg
     2221 ctatggtgga aaaagccaac tccgggcacc ccggaggagc aatgggcggt gctgatttca
     2281 ttcacatcct cttttctgaa ttcctgaggt ttgatccggc tgatcctgaa tggatcaacc
     2341 gtgatcgctt cttcctggat cccggacata tgtcgccgat gctctattcg gtgctgacac
     2401 ttaccggtca ttttaccgtt gatgacatca tgcagttccg gcagtggggc agcgtgacac
     2461 ccggacatcc cgaactggat ataaaacggg gtgtagagaa cacctccgga cctctcggac
     2521 agggtcatac catggccgtc ggcgccgcca tagcggaaaa gttccttacg gccaggttcg
     2581 gaaggctgtt tgaacacagg atttatgcat atatctctga cggaggaatc caggaggaga
     2641 tctcccaggg agccgggagg cttgcaggtc atctcgggct gagtaacctg gtgatgttct
     2701 atgactccaa tgatatccag ctctcaaccg agacctcggc agtgacttct gaagacaccg
     2761 cagccaagta caaggcttgg ggatggcgtg tgatggagat agacggccac agtcatgacc
     2821 agatacgggg cgcactccgg atggc
//
LOCUS       DAOPXU010000188         2824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_49602_length_2824_cov_12.529433, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000188 DAOPXU010000000
VERSION     DAOPXU010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49602_length_2824_cov_12.529433"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..187
                     /gene="rpmD"
                     /locus_tag="PKK03_12210"
     CDS             8..187
                     /gene="rpmD"
                     /locus_tag="PKK03_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNX85203.1"
                     /translation="MAKIKVTQIKSKIGQPESQKKILTALGIRKMHQTVEHEDTPQIM
                     GMVNKLKHLVKVEKL"
     gene            215..661
                     /gene="rplO"
                     /locus_tag="PKK03_12215"
     CDS             215..661
                     /gene="rplO"
                     /locus_tag="PKK03_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNX85204.1"
                     /translation="MDLSNLKPAPGSVKREKRIGRGEGSGHGGTSTRGHKGAKSRSGY
                     SRKIGFEGGQMPLQRRVPKSGFKNLNRVDYKAINLSTLEALATKLDTDRIDFETLLTA
                     GLVNKNALVKILGKGTLTRKLEVHAHAFSKSAQAAIEAIQGTAVKL"
     gene            665..1993
                     /gene="secY"
                     /locus_tag="PKK03_12220"
     CDS             665..1993
                     /gene="secY"
                     /locus_tag="PKK03_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNX85205.1"
                     /translation="MKFIQTIKNIFKIEDLRARIIFTLGIVALYRLGKYITLPGVDPS
                     MLDNLRSQTSSGIMGLLDMFSGGAFSQASIFALGIMPYISASIVIQLLGIVFPYFQKL
                     QKEGESGRRKMNQYTRYLTVFILILQAPTYLANLHAILPPEAFVLQGWFFRVSSVIIL
                     TAGTIFVMWLGEKITDKGIGNGISLIIMVGIIARFPVAIIFETGSRMNGSGGLVALLI
                     EIVFLFVVILGSVLLVQGTRRIPVQYARRIVGNKQYGGVRQYIPLKVNAAGVMPIIFA
                     QAIMMLPVIFAGFRESGSGFVVAFTNMYGFWYNLVTAILIILFTYFYTAVTINPVQMA
                     EDMKKNGGFIPGIKPGRKTVEFLDTIMSRITLPGSIFLAIIAILPAFAVNFGISGEFA
                     QFFGGTSLLILVGVVLDTLQQIESHLLMRHYDGLMKSGRVKGRTGAVTAI"
     gene            2011..2787
                     /gene="map"
                     /locus_tag="PKK03_12225"
     CDS             2011..2787
                     /gene="map"
                     /locus_tag="PKK03_12225"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNX85206.1"
                     /translation="MIYIKTDAEVELLREANQLVSKTLAEVAVHIRPGVTTLRLDAIA
                     EEFIRSHGALPAFKGYAGFPNTLCTSVNDEVVHGIPSDYALREGDIISVDCGVILNGW
                     YGDSAFTFPVGEVRPEILRLLDFTRASLEEGVKQAVAGNRVGDISHAVQTKAEGGGYS
                     VVRTLVGHGIGKHLHEGPEVPNFGRRGSGTRLERNVVICIEPMINMGTKNTRTDSDGW
                     TVRTGDGKPSAHFEYAVAVREGKPDLLTTFEYIEEVLTKR"
BASE COUNT          743 a          709 c          736 g          636 t
ORIGIN      
        1 aagtatcatg gcaaaaatca aggtaaccca gattaaaagc aagataggcc agcccgagtc
       61 acagaagaag atcctcacgg cactgggtat cagaaagatg caccagactg ttgagcacga
      121 agacacaccc cagatcatgg gtatggtaaa caaactgaaa cacctggtga aggtggagaa
      181 actctaagat attaacagca ttatatattt cgctatggat ttaagcaatt tgaaaccggc
      241 ccccggatca gtcaagagag agaagcgcat cggacgaggt gagggatcag gtcacggagg
      301 aacatcaacc cgcggacaca aaggggccaa gtcacgctcc ggctacagca ggaagattgg
      361 cttcgaagga ggccagatgc cactccagag aagagtgccg aaatcagggt ttaaaaacct
      421 caaccgcgtt gactacaaag caatcaacct cagtacactt gaggctctgg caacaaagct
      481 tgacacggac cgcattgatt ttgagactct gctgacagca gggctggtca acaaaaatgc
      541 acttgtgaag atactgggca agggtaccct taccaggaag cttgaggttc atgcccacgc
      601 tttcagcaaa tcggcacagg cagcaatcga agcaattcag ggaaccgcag ttaaactgta
      661 aggcatgaaa ttcattcaga ccattaagaa cattttcaag atcgaggatc tgagagccag
      721 gataatcttt acccttggca tcgttgcact ctaccgtctg ggcaaatata tcaccctccc
      781 gggagtcgat ccatccatgc ttgataacct gagaagccag acctcctcgg gtattatggg
      841 gctgcttgac atgttctcgg gcggagcctt ctcacaggcc tccatctttg ccctggggat
      901 catgccctac atctccgcct caatcgttat ccagctgctc ggcatagtct tcccctactt
      961 ccagaaactt cagaaggagg gtgagagcgg caggcgtaag atgaaccagt atacacggta
     1021 ccttaccgta tttatcctga tacttcaggc tccgacttac ctggcaaacc ttcatgcaat
     1081 attgcccccg gaagcatttg tcctgcaggg ctggttcttc agggtctcat cggtgatcat
     1141 attgaccgcc ggaacaatat ttgtcatgtg gctcggtgag aaaatcaccg acaagggtat
     1201 cggaaacggt atctcgctga ttatcatggt tggtattata gcccgtttcc cggtggcaat
     1261 catctttgag acaggatcac gcatgaacgg ctcgggcgga cttgtagccc tgctcattga
     1321 gattgtattc ctctttgtag tgatacttgg ttcagtgctc ctggtacagg gcacccgccg
     1381 catacccgtc cagtatgcaa gacgcatcgt gggcaacaaa cagtatggcg gcgtaagaca
     1441 atatattccc cttaaggtga acgcagccgg cgttatgcct atcatcttcg cccaggcgat
     1501 catgatgctg ccggtgatat tcgccggttt cagggaatca ggctcgggat tcgtcgttgc
     1561 atttacaaat atgtacggat tctggtataa cctggtgaca gcaattctca tcatcctctt
     1621 cacctacttc tacaccgccg ttaccattaa cccggtgcag atggctgaag acatgaagaa
     1681 gaacggtggt ttcattcccg gcatcaaacc gggacgcaag acggttgagt tccttgatac
     1741 catcatgtca cgaatcaccc ttccgggttc aatattcctc gcaataatag ccatcctccc
     1801 tgcctttgcc gtgaacttcg gcatcagtgg tgagtttgca cagttcttcg gcggcacctc
     1861 gctcctcatt ctcgtgggcg ttgtgcttga cactctccag cagatcgaaa gtcatctgct
     1921 catgcgccac tatgacggcc ttatgaaatc aggccgcgtt aagggcagga ccggagccgt
     1981 gacggctatt taatgcgatt tcgttctttg atgatctata ttaagacgga tgcagaagta
     2041 gagttactga gagaagccaa ccagctggtc tcaaaaactc ttgcggaagt agccgtgcat
     2101 atcaggccgg gggtgacaac cctccgtctt gacgcgatag ctgaggagtt catccgttca
     2161 cacggtgcac tccctgcctt caagggatat gccggatttc cgaacaccct ctgcacatct
     2221 gtaaatgatg aggtggtaca cgggataccg tcagactatg cactcaggga gggcgacata
     2281 atatcagtcg actgcggggt gatactgaac ggctggtatg gagacagcgc cttcaccttc
     2341 ccggtgggag aggtgaggcc tgaaatactg cgtcttctcg atttcaccag ggcctctctt
     2401 gaggagggcg tgaagcaggc tgtcgccggc aaccgcgtgg gagacatttc gcacgcagtg
     2461 cagacaaagg ctgaaggcgg aggttactcc gtggtgcgca cgctggtggg acacggaatc
     2521 gggaagcacc ttcatgaggg ccccgaggtg cctaacttcg ggagacgcgg ctctggcacg
     2581 agacttgaaa ggaatgtggt catatgcata gaaccgatga tcaatatggg taccaaaaac
     2641 acacgcactg acagtgacgg gtggacagtc agaacaggtg acggcaaacc gtcggcacat
     2701 tttgaatatg ctgttgcggt cagggaggga aaacccgacc tgctcacaac atttgaatat
     2761 atagaggaag tattaacaaa gaggtaagta tatggccaaa caaccatcaa tagagattga
     2821 cgga
//
LOCUS       DAOPXU010000189         2814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_49887_length_2814_cov_11.413193, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000189 DAOPXU010000000
VERSION     DAOPXU010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49887_length_2814_cov_11.413193"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..1596)
                     /locus_tag="PKK03_12230"
     CDS             complement(82..1596)
                     /locus_tag="PKK03_12230"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNX85207.1"
                     /translation="MKRTIIITSIIVAVAVIAMIVISKLTGKKDMSTLFTEVRQGDFE
                     VVVTTTGELQAEVSTDIKGPEGLNSRNMRFGSIKISDLVPEGTEVKAGDYVATLDRSS
                     VDNSLKDELDRLESYETDLLKKQLDTTISLGNLRDELLNLKFNMDEAEITLEQSKYEP
                     PTTIRQARISLDKAQRSYEQAVSNYDLKVRQAKADIWDTELQVSKQRRKVTEMQAIIQ
                     QFVISAPSDGMVIYKREWSGAKRKVGSEISGWDPVVATLPDLTSMISKTYVNEIDVSK
                     VKAGQRVRLTVDAFPEKTYTGTVISVANIGEQLPNTDAKVFEVITKIDGSDPILRPSM
                     TTGNQIITKTFNDVTFIPLETVFATADSIPFVYKKDGSRQVVVLGESNENDVIVEQGL
                     KEGDKLYLTIPEDADRFKLQGDDLIAVIKERKKQAAEEEAKRQQNGNRPQGMMPGSMT
                     PEQMREFMQNLTPEQREAMRQQRGAGRPGQMQGRPAGSGSDTTVRVQRVRTPNQ"
     gene            complement(1759..>2814)
                     /locus_tag="PKK03_12235"
     CDS             complement(1759..>2814)
                     /locus_tag="PKK03_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNX85208.1"
                     /translation="AQWNALKLLNEDGEFLSDADGREVLALAEAEDFDYVTYDRLGTE
                     TGDDSWTEKHIRMVTGMTLTDCDAIRKAGFTVAVDAVSSVGGIAIPALLRALGVRKVI
                     ELYCEPTGLFPHNPEPLPEHLSDISRTVKESGAHLGFVVDPDVDRLAIICEDGSMFGE
                     EYTLVSVADYVLSHVKGPTVSNMSSTRALRDVTESHGCSYTSAPVGEVNVVAEMKRTG
                     AVIGGEGNGGVIYPALHYGRDALAGIALFLTHLAKQGVRCSELRKKYPDYYIAKKKID
                     LPGRIEMEELREKLFMAYPEADCDTRDGIKLDLPTGWVQIRKSNTEPIMRIYAEERTM
                     EQAEKLAADVTVIVKNL"
BASE COUNT          597 a          791 c          648 g          778 t
ORIGIN      
        1 tcaagggcga tgcgtatgtc gtgtccgtat cttttgaaca tgttattcca tgtttgtttg
       61 cctcagaatc ctgtattcct gctactggtt gggagttctt acccgctgca ccctgacggt
      121 ggtatcgctg ccgctgccag ccggtctgcc ctgcatctgg ccggggcgtc cggcaccgcg
      181 ctgctgacgc atggcttcac gctgctcggg ggtcagattc tgcatgaact cacgcatctg
      241 ctccggggtc atgcttccgg gcatcatacc ctgaggccgg ttgccgttct gctgacgctt
      301 ggcttcctcc tcggccgcct gcttctttct ctccttgatg actgcaatca gatcatcacc
      361 ctggagcttg aacctgtcgg catcttccgg aatcgtcagg tataacttgt ctccctcctt
      421 caggccctgc tccactatca catcattctc gttcgactca ccgagcacca ccacctggcg
      481 tgatccgtct ttcttataga caaaggggat actgtcagcc gtggcaaaca ctgtctccag
      541 cggaataaag gtaacgtcat tgaatgtctt ggtaattatc tggttgcccg tggtcattga
      601 gggcctcaat atgggatcag atccgtcaat tttcgttatt acctcaaata ccttggcatc
      661 agtgttgggc agctgttcgc cgatgttggc aacactgatc accgtacctg tgtatgtctt
      721 ctcggggaag gcgtcaacgg ttaacctgac acgctgaccg gcctttacct tgctgacatc
      781 aatctcgttg acatatgtct tggatatcat cgatgtgagg tcgggaagag ttgccactac
      841 cggatcccaa ccggatattt ccgaacctac cttgcgcttg gcaccgctcc actctctctt
      901 gtagatgacc atcccgtcag acggagctga tatgacaaat tgctggatga tggcctgcat
      961 ctcggtaacc tttctccgct gctttgaaac ctgaagttcc gtatcccaga tgtcagcctt
     1021 tgcctgcctt accttaaggt catagttgct gaccgcctgc tcataagaac gctgggcttt
     1081 gtcaagtgat atcctggcct gcctgattgt agtcggaggc tcatattttg actgctccag
     1141 ggtaatctca gcttcatcca tattgaactt gagattcagg agctcatctc tcaggttgcc
     1201 gagagaaatt gtggtatcaa gctgtttctt cagcaggtcg gtttcataag actccagcct
     1261 gtcaagctca tccttgaggc tgttgtcaac actcgagcgg tcaagcgtgg cgacataatc
     1321 accggccttc acctcggtac cttcaggcac caggtcagag attttgattg atccgaaccg
     1381 catgttgcgg ctgttaagac cctccggtcc tttaatatca gtcgaaacct ctgcctgcag
     1441 ctcaccggta gtggtgacaa ccacctcgaa atcaccctga cgaacctcgg taaacagtgt
     1501 gctcatgtct ttcttgcccg tcagcttgct tatgacgatc atggcaataa cagccaccgc
     1561 tacaatgatt gaggtaatga taatagtcct tttcatctgc tgaattatct gtttgtatta
     1621 ttaaagccgt gaaattatta taaatttagg cttactgtgt tctttttaag gaaacattaa
     1681 ctttcattaa cttctgcccg ttgattgaga acaaaaactg ttcaaaaaag atctgaagtc
     1741 tgctccggag agccagattt atagattttt aacaatgaca gtaacatcag ctgccagttt
     1801 ttcagcctgt tccattgttc tctcctcggc ataaatacgc atgataggtt cggtgttgga
     1861 tttccggatc tgtacccacc ccgtcggaag gtcgagtttt atcccgtcgc gggtgtcaca
     1921 gtcagcctcc gggtatgcca tgaacagctt ttcgcgaagc tcttccatct caatacggcc
     1981 cggaagatct atcttcttct tggcaatata ataatcggga tatttcttcc gcaattcact
     2041 gcatctgaca ccctgttttg ccaggtgtgt caggaagagg gctattcccg ccagggcatc
     2101 acgtccgtaa tgcaacgccg ggtatatgac tcctccgttg ccttctccgc ctatgacggc
     2161 tcctgtgcgc ttcatctccg caaccacgtt aacctcaccc accggtgctg aggtatagct
     2221 gcagccatga ctttcagtca catccctcag cgccctggtt gatgacatgt tggataccgt
     2281 ggggcccttg acatggctca gaacatagtc tgcaactgat accagggtat actcttctcc
     2341 gaacattgat ccgtcttcgc atataatggc cagcctgtca acatcagggt caaccacaaa
     2401 cccgagatgt gcaccgctct ccttcactgt ccgggagata tctgacagat gctccggcaa
     2461 tggttcgggg ttgtgcggaa agaggccggt aggctcacag taaagctcaa tcacctttct
     2521 gacgcccagg gctctcagaa gtgcaggaat ggctattcct cccaccgagc tgacagcatc
     2581 aacggcaacg gtaaacccgg ccttccggat agcgtcacag tcagtcagtg tcatcccggt
     2641 taccatcctg atatgctttt cagtccagct gtcatcaccc gtttcagtac ccagtctgtc
     2701 ataggtcaca tagtcaaagt cctccgcttc ggccagggcc agaacctccc tgccatcagc
     2761 atcactgagg aactctccgt cttcattgag cagcttgagc gcgttccact gtgc
//
LOCUS       DAOPXU010000190         2793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_50481_length_2793_cov_11.667275, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000190 DAOPXU010000000
VERSION     DAOPXU010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50481_length_2793_cov_11.667275"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..564)
                     /locus_tag="PKK03_12240"
     CDS             complement(22..564)
                     /locus_tag="PKK03_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNX85209.1"
                     /translation="MRIIGGKYRGRLIEPPSGFAARPTTDFAREGLMNILNNRYDFSA
                     ISLLDLFGGTGAISFEFASRGTNDIDVVETDRRSCEFISRTLRDLGPEHARVHRLDVR
                     DWLKICHKQFDIIFADPPYALRWLTDIPDMVMASGAVHNSTLFILEHPKSMDFSGHPL
                     RTDHRKYGNVNFSFFNIPAS"
     gene            complement(555..1412)
                     /locus_tag="PKK03_12245"
     CDS             complement(555..1412)
                     /locus_tag="PKK03_12245"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HNX85210.1"
                     /translation="MTASQIYPLELIDETLDINATDNYDLTLELSEEGVSLAVLDLLR
                     GKYVMLRHYPLEGPPEGTVRTYTEILDSDDFLRRRYRKIRIITPSLNSTMIPAPVYEP
                     GLREDYFRFNFGITVEDGIVANNVPFPDAVVLFSPGREISGIIPSRWHGVTPWHHTKP
                     LLAHVSAASRNADDCYIHLHLEKNFATVIIAEKRNLTFCNSFAISSPQDGAYFLFSVL
                     DRKGIRHDETICISGLVEPYSETHMALLNFSTGIRFASPLIRQSFSYVMNEVRLHRWL
                     NLFTAASCE"
     gene            complement(1412..2047)
                     /locus_tag="PKK03_12250"
     CDS             complement(1412..2047)
                     /locus_tag="PKK03_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85211.1"
                     /translation="MKTALNIVFTLIIIVLAYFLFESIMKPIRFKNERESRESAIKEK
                     LIDIRTAQEAFKSVKGYYTGSFDTLITFLKTDSLPLVFKIGSLTDEMIASGITTEREA
                     VKKGLISRDTSYIPVRDSIFDKGYPIDQLRFVPGMEKTEFQMSAGRVMTTSMVLVNVF
                     EAYVLNDVFLSDLDRQLVVNYNDQRTKITGFPGMKVGDVKVPNNNAGNWED"
     gene            2127..>2793
                     /locus_tag="PKK03_12255"
     CDS             2127..>2793
                     /locus_tag="PKK03_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX85212.1"
                     /translation="MRQENIYNLLVKNLGYMPTQDQSDCMRLLSGFVADNTNDVIFLM
                     TGYAGTGKTSVIASLVATLNTLRQKSVLLAPTGRAAKVLRSYTGMEAYTIHRKIYRQK
                     SATDGVGKFVLDRNLHKETYFIVDEASMIPDESSEGSMFGSGRLLDDLLEYVYTGADC
                     KLILVGDVAQLPPVGSIVSPALDENVLRSTGFALQMFELRQVIRQSEGSGILMNATAV
                     RQQV"
BASE COUNT          676 a          650 c          757 g          710 t
ORIGIN      
        1 gtgaaggttg aaatgtgcag attatgaagc gggtatgtta aaaaaactga agtttacatt
       61 tccgtatttt cggtggtcag tccgtaaggg gtgccccgaa aaatccattg atttcgggtg
      121 ttcgagaatg aatagtgttg agttatgcac tgctcccgaa gccattacca tgtcagggat
      181 atcggtgagc cacctgagag cgtatggcgg gtctgcaaag atgatatcaa actgtttgtg
      241 acagatcttc agccagtctc gcacgtcgag gcggtgaacc cttgcatgtt cagggccgag
      301 gtcccggagg gttctgctga tgaattcgca gctgcgccgg tcagtttcta caacatcgat
      361 atcattggtt ccccgggatg caaactcgaa gctgattgct ccggttcctc cgaagaggtc
      421 gagcagggag attgctgaga agtcatacct gttattcagg atattcatca gcccttcgcg
      481 ggcgaagtct gtggtgggcc tggcggcgaa gcctgacggg ggttcaataa gcctccccct
      541 gtatttcccg cctattattc gcacgaggcc gcagtaaaga ggttcagcca ccggtgcagg
      601 cgcacctcgt tcatgacata gctgaagctc tgcctgatga gaggagaggc gaaccttatg
      661 ccggtggaaa agttcaggag cgccatgtgc gtttcgctgt atggctcaac caatccgctg
      721 atacagatgg tttcgtcatg ccggatgccc ttcctgtcaa gcacgctgaa gaggaaatag
      781 gccccgtcct gcggtgatga aatggcgaag ctgttgcaga aggtgagatt ccgtttttcc
      841 gcaatgatca ccgtggcgaa attcttttca aggtggagat gtatataaca gtcatcagcg
      901 tttctgcttg ctgccgacac gtgggcgagc agcggtttcg tgtgatgcca gggcgtcacg
      961 ccgtgccatc gcgaagggat tatcccggat atttccctgc ccggagaaaa gagcacaact
     1021 gcatcgggaa agggaacgtt attggctact atcccgtctt cgacggttat gccgaagttg
     1081 aaccggaaat aatcctcgcg tagaccgggc tcataaaccg gcgccgggat cattgtggaa
     1141 ttcagtgacg gagtgataat gcggattttc ctgtaacgtc ttctgaggaa atcatcggaa
     1201 tcaagaatct cagtgtaggt ccggactgtt ccttccggcg gcccttcgag gggataatgc
     1261 ctcagcatta catacttgcc ccgaagaagg tcaagcactg caagtgagac gccctcttca
     1321 gaaagctcaa gcgtaaggtc gtaattatct gtcgcgttta tgtcgagggt ctcgtcaatg
     1381 agctccaggg gatatatctg cgacgcggtc atcaatcctc ccagttcccg gcattgttat
     1441 tgggcacttt gacatcaccc accttcatgc ccggaaaccc cgtgattttg gttctctgat
     1501 cattgtagtt gacaaccagt tgcctgtcaa ggtcgctgag gaagacatca ttgagaacgt
     1561 acgcttcgaa cacattgacc agaaccatgg aagttgtcat cactcttccg gctgacatct
     1621 gaaattcagt tttctccatt ccgggaacaa acctgagctg atctataggg taacctttgt
     1681 cgaatatgga gtcacgtacg gggatgtagc tggtgtcacg gctgataaga cctttcttaa
     1741 cggcttctct ctcggtggtg attccggatg caatcatctc atcggtcagc gaacctatct
     1801 tgaacacaag aggaagcgaa tcagttttga ggaatgttat aagggtgtca aaactgcctg
     1861 tgtaatatcc ctttactgat ttaaaagcct cctgggcagt cctgatatca ataagtttct
     1921 cttttattgc agattccctg gattcccttt catttttgaa gcggataggc ttcatgatgc
     1981 tttcaaaaag aaaataggcc agcacgataa tgataagggt gaatacgatg ttgagagccg
     2041 ttttcatgac ttttatgatc gattgttgaa taactttgtg caaatttaaa aaaaaagcag
     2101 acggttatca ggtttgtcga aataaaatga gacaggaaaa catctacaat cttctcgtta
     2161 agaatctggg ttacatgccc acgcaggacc agtctgactg catgcgtctg ctgtccggat
     2221 tcgttgccga caacaccaat gacgtgatct tcctgatgac cggatatgcc ggtaccggga
     2281 agacgagcgt aattgcctcg cttgtagcga cgctgaacac actccgccag aagtcggttc
     2341 tgcttgcccc aaccggaagg gcggcaaagg tgctgaggtc ctacacgggc atggaggctt
     2401 acaccattca ccggaagatc tacaggcaga agtcggccac tgacggggta ggcaaatttg
     2461 ttctggaccg taaccttcac aaggaaacct atttcattgt cgatgaagct tcgatgatcc
     2521 ctgatgagtc atcggaaggc tcgatgtttg gaagtggccg gctgctggat gaccttcttg
     2581 agtatgtgta tacgggagct gactgcaagc tgatcctggt gggcgatgtt gcgcagttgc
     2641 caccggtagg atcaattgtg agtccggcac tggatgagaa tgtgctgcga agcaccggtt
     2701 tcgccctgca gatgtttgaa cttcgccagg tgatcaggca gagcgaaggg tccggtatac
     2761 tgatgaatgc cacagctgtc aggcagcagg taa
//
LOCUS       DAOPXU010000191         2760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_51387_length_2760_cov_10.249168, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000191 DAOPXU010000000
VERSION     DAOPXU010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51387_length_2760_cov_10.249168"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="PKK03_12260"
     CDS             complement(<1..1240)
                     /locus_tag="PKK03_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HNX85213.1"
                     /translation="MAAEIEESILMTTFPADTVFITDFGAVADDASFLNTGAIAAAIL
                     KCHSEGGGVVFVPEGRWTTGPITLMSNVNLHIAKNATLAFSTDYELYLPPVLTRWEGM
                     DCYNIHPLIYARDASNIALTGKGTIDGQASNEAWWWMNGNPDYGWREGMNNQVLSGRI
                     RLLAYEQNQTPVEERRFDLGGALRPQLINFMRCNTVLIEDLTLRNSPFWVIHPVLTDN
                     FILRGVTIRSAGPNSDGCDPESCNGVLIENCRFDTGDDCIAIKSGRNNDGRRWSVPSQ
                     NVIVRNCRMEDGHGGVVIGSEISGGFKNLFVEDCEMDSPELLRVIRIKTSYCRGGVIE
                     NINVRNVKVGRCEEAVLKINLMYEPRENCLRDFPPYVRNVTLENVTSARSRYGVYIIG
                     LDSMVNVSDVTVRNCTFNGVE"
     gene            complement(1456..2655)
                     /locus_tag="PKK03_12265"
     CDS             complement(1456..2655)
                     /locus_tag="PKK03_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HNX85214.1"
                     /translation="MKKVQSLICCLLAMTLFLSCGSSRNSGLDSPLWSVRMAESEMIR
                     NPSLWMADFVTEKKWNYTQGLMGLAFLKLYDVTDDKRYFNYAKGYADSLIDADGIIYK
                     YRMDNYNLDQVNPGKMMFILYDRTGDPRYEKVIQTLYRQLQNQPRIAEGGFWHKKVYP
                     DQMWLDGLYMSAPFYAEYALRYNHPDDFRDVVNQFLIIASRTYDPVTGLYRHAFDASK
                     KMAWADSITGQAPHAWGRAMGWFSMAIVDVLDFLPVDQPGRNELTGILQGMVQKLSEI
                     QDPATGGWYQVPDQSGAEGNYIETSCTAMFAYSIFKGVRMGYLDGKYLDIAEKAYQGL
                     LDNFIVTNPDGTVTMKNICGVAGLGGNPYRDGSYEYYIGEVIRDNDPKGVGPFILASL
                     EHERIRK"
BASE COUNT          643 a          741 c          664 g          712 t
ORIGIN      
        1 cctccacacc gttgaaggtg caatttctga ccgtcacatc cgaaacgttg accatactgt
       61 caagacctat aatataaacg ccgtacctgc tccgggctga agtcacattc tcaagcgtca
      121 cattccttac atagggcggg aagtctctca ggcagttttc ccgaggctca tacatgagat
      181 tgatcttcag cacggcctcc tcacacctgc caaccttcac attgcgtaca ttaatgttct
      241 ctattacacc gccccggcag taggatgtct tgatacgtat cactctcagc aactccgggc
      301 tgtccatctc gcagtcttca acgaagagat ttttaaatcc gccggagatc tcgcttccga
      361 tcactacccc gccatgaccg tcttccatcc tgcagtttcg tacgataaca ttctgcgacg
      421 gcactgacca tcttctgccg tcgttgttcc tgcccgactt gatggctata cagtcatcgc
      481 cggtgtcaaa gcggcagttt tctatcaaca caccgttgca tgattcagga tcacaaccgt
      541 cagagtttgg acctgcactc cggatggtca cccccctgag gatgaagttg tcagtcagta
      601 ccgggtgaat cacccagaag ggagagttcc tgagggtcag gtcttcgatc agcaccgtgt
      661 tgcagcgcat gaagttaatc agttgcggtc tgagcgctcc ccccaggtca aaccggcgtt
      721 cctcaacagg agtctggttc tgttcatacg ccagcagccg gatccttcct gagagtacct
      781 ggttgttcat cccctcacgc cagccgtaat cgggattgcc gttcatccac caccatgcct
      841 cgtttgaagc ctggccgtca atggtgcctt tcccggtaag ggcaatgtta gaagcatccc
      901 ttgcataaat aagagggtga atattgtagc agtccattcc ttcccagcgt gtcagaacag
      961 gagggaggta cagctcataa tcagttgaga aagccagtgt ggcattcttg gctatgtgaa
     1021 ggttaacgtt gctcatcaat gtgatgggac cggttgtcca tctcccttcc gggacgaaca
     1081 ccacacctcc tccttcgctg tggcatttca ggatggcagc agcgatggct ccggtattga
     1141 ggaaggatgc atcgtcagcc acagcgccga agtcagtaat aaatactgta tctgcaggaa
     1201 aagtcgtcat aaggattgac tcttcaatct ctgcagccat cttccagttg tcatcttcag
     1261 tacaggatgc caatacggca acgaggaaga tcaataaaac cggtttaata cattgcatga
     1321 tcagcaacct gttttttttc tgaaacaaag gtatatctgc accctgcaga ggcagtacac
     1381 ttttttggca aaagggtgga gaatttggtc ggagactttc agactttcag actttcagac
     1441 tttcagactt tccttctact tcctgatcct ctcgtgttcc aggctggcca ggataaaggg
     1501 tccgacgccc ttggggtcat tgtcacggat cacctcgccg atatagtatt catacgagcc
     1561 gtcgcggtaa ggattgcctc ccaggccggc tactccgcag atattcttca tggtcaccgt
     1621 accatccgga tttgtaacga tgaagttgtc caggagaccc tggtacgcct tttccgcgat
     1681 atcaaggtat ttaccgtcaa gatagcccat ccgcaccccc ttgaaaattg aataggcgaa
     1741 catggcagta cacgatgttt caatgtagtt tccttcagcg ccggactggt caggcacctg
     1801 gtaccatccg ccagtggcag ggtcctggat ttctgacagc ttctgcacca tgccctgcag
     1861 tatcccggtc agttcattcc ttccgggctg atccacaggc aggaaatcga gtacatcgac
     1921 tattgccatt gagaaccacc ccatggctct tccccaggcg tgaggagcct ggccggtaat
     1981 gctgtcggcc caggccatct tcttcgatgc gtcaaaggca tgcctgtaaa ggcctgtgac
     2041 aggatcatat gtgcgggaag caataatcag gaactgattc accacatcac ggaaatcatc
     2101 aggatggtta taccttaaag cgtattcagc ataaaaaggt gcgctcatgt aaagtccgtc
     2161 aagccacatc tggtcagggt agaccttctt gtgccagaag cctccttcgg ctatccgcgg
     2221 ctgattctgc aactgccggt aaagagtctg aatcactttt tcgtatctcg gatcaccggt
     2281 acggtcgtaa aggatgaaca tcatcttgcc cggattgacc tggtcaaggt tgtagttatc
     2341 catcctgtac ttataaatta ttccatcggc gtcaatcagg gaatcggcgt aaccttttgc
     2401 gtaattaaaa taccgtttgt catcagtgac atcatagagc ttcaggaagg caaggcccat
     2461 cagcccctgg gtgtagttcc actttttttc tgtcacgaaa tcggccatcc agagcgaggg
     2521 gttccggatc atctcggact cggccatccg tactgaccaa agcggcgagt caagcccgct
     2581 gtttctgctg cttccgcagg agaggaacag ggtcatggca agcaggcagc aaatgagtga
     2641 ttggactttt ttcatgaaga tggtaaaatt ttgccgggct cagccctgtt tctgtttcac
     2701 gtgtttgtcc ctgtattctg tgggagtcat tgcgaagcgt tgtttgaaga tgcgcgagaa
//
LOCUS       DAOPXU010000192         2708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_52856_length_2708_cov_15.150396, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000192 DAOPXU010000000
VERSION     DAOPXU010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52856_length_2708_cov_15.150396"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2333)
                     /locus_tag="PKK03_12270"
     CDS             complement(<1..2333)
                     /locus_tag="PKK03_12270"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNX85215.1"
                     /translation="MKIDRLLVGIVLILCALTTFPALAQDGDQILDGIGETGLISRYQ
                     FKGNDRDWSRNNLHAITRGTDFRFIPDSLFGNVLALSGESETYVSIPGEAVSGEESLT
                     ITGWIWLRDAKPGQLFFDFGKDSNSHFFAAPAGTKEKPGLQTAFVAGSKTYTTSFDAV
                     PLNKWSHLAVVLDIPAKTMSTYLNGMLVVEVKDAALDISQLFDNDSEKNNQLYVGKSI
                     DGEKTFLNAKLSDFRIYRIPLTPRQIGRIYFTALRGGNPQAGRRGQPEQTLQVFPVTT
                     PQLYNAFLVSVPDTQVETTAGNLPRLPRFVKGVYGNGVDGPDVRVIWPVPEDNSQALV
                     PGTYTVTGKVSGTSLQPKAVVTVRAAVAPVAPHRTLEAFRLDQVTLDADIHNHETKFI
                     ENRDKFLKGLEATNPDNFLYMFRNAFGQKQPEGAQPLGGWDSQQTKLRGHATGHYLSA
                     IAQAYASTGYNAELRAKFAGKMEYMVNTLYELSQMSGQPRTAGGEFSADPTAVPPAAG
                     KTGYDSDLSEAGIRTDYQNWGKGFISAYPPDQFIMLEKGATYGGSNAQVWAPYYTLHK
                     IMAGLMDIYEVSGNEKALETAKGMGTWVYARLSQLPEETLISMWNRYIAGEFGGMNEA
                     LARLSRITGDTSYMKAAQLFDNIRLFYGNADHTHGLAKNVDMFRGLHANQHIPQIIGA
                     IEIYRDTESPEYYHIADNFWDMVTHDYMYSIGGVAGARNPNNAECFTAQPASLYENGF
                     AAGGQNETCATYNMLKLTGSLFLFNQDAALMDYYERGL"
     gene            complement(2449..>2708)
                     /locus_tag="PKK03_12275"
     CDS             complement(2449..>2708)
                     /locus_tag="PKK03_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX85216.1"
                     /translation="VLKRLNVSPSCITDVIITHPHPDHIGGIHLFPNARVWMQKEDFV
                     YFVSGAWKKEGALSEFTDNDVRNLTEISLLGKLELTNFIYL"
BASE COUNT          587 a          763 c          702 g          656 t
ORIGIN      
        1 agcccgcgct catagtaatc catcagcgcc gcatcctggt tgaagaggaa gaggctcccg
       61 gtcagcttga gcatgttgta ggtggcgcag gtctcgttct gtccgcctgc ggcgaaaccg
      121 ttctcataca acgatgccgg ctgcgcggtg aagcactcag cattattcgg attgcgtgca
      181 cctgcaacac caccgatgct gtacatgtag tcatgggtga ccatgtccca gaagttgtca
      241 gcgatgtgat aatactcagg cgattcggta tcacggtaaa tctctatcgc cccgatgatc
      301 tgcggaatat gctggttggc atgcagccct cggaacatat ccacattctt cgccagcccg
      361 tgcgtatgat cagcattgcc atagaacagc ctgatattgt caaacagctg agcagccttc
      421 atgtagctgg tatcaccggt gatacgcgac aaccgtgcaa gcgcctcgtt catcccgccg
      481 aactccccgg cgatgtacct gttccacatg cttatcagcg tctcctccgg cagctggctc
      541 aggcgggcat atacccatgt acccatcccc ttcgctgtct ccagcgcctt ctcgttgccg
      601 ctcacctcgt aaatatccat cagcccggcc atgatcttgt gaagggtata gtacggcgcc
      661 catacctggg catttgagcc gccataggtg gctcccttct ccagcatgat gaactgatca
      721 ggcggatagg cgctgataaa acccttgccc cagttctggt aatcggtgcg tatccctgcc
      781 tcgctcaggt cagagtcgta gcctgttttt cccgccgcag gcggaacggc tgtcggatcg
      841 gccgaaaact ctccgccggc cgtccggggc tggcccgaca tctgcgacag ctcataaagc
      901 gtattcacca tatactccat cttgccggcg aactttgccc ggagctccgc attataaccg
      961 gtgcttgcat aggcctgggc gatggcgctg aggtaatgcc cggttgcgtg accgcgtagc
     1021 ttggtctgct ggctgtccca cccgccgagc ggctgcgcac cctctggctg cttctgcccg
     1081 aaggcattgc ggaacatgta aagaaaatta tccggattgg tggcctcgag gccctttaaa
     1141 aacttgtcac ggttctcgat gaacttcgtc tcgtgattgt ggatgtcagc atccagcgta
     1201 acctggtcaa gcctgaacgc ttcgagggtg cggtgcggtg caacaggcgc cacggctgcc
     1261 ctgacagtca ccactgcctt cggctgcagg ctcgttccgg aaacctttcc tgtcaccgtg
     1321 tatgtcccgg ggacaagcgc ctggctgtta tcctccggga ccggccagat cactctcacg
     1381 tcgggaccat ccacaccatt gccgtaaaca cctttcacaa aacggggcag cctggggagg
     1441 tttcctgccg tggtctcaac ctgggtgtca ggaacactta ccaggaacgc attgtacagc
     1501 tgcggcgttg ttaccggaaa aacctgcaga gtctgttcag gctggcccct gcgccctgcc
     1561 tgcggattgc cgcctctgag agcggtgaaa tagatcctgc cgatctgcct cggtgtcaat
     1621 ggaatacggt aaatacggaa gtcggagagc tttgcgttca ggaaagtctt ttcaccgtcg
     1681 attgatttgc caacatagag ctggttattc ttttccgaat cattgtcgaa cagctgtgaa
     1741 atgtccagtg ctgcatcctt aacttcaacg accagcatgc cattgaggta tgtactcatg
     1801 gtcttcgccg ggatgtcaag aaccactgcc aggtggctcc atttgttcag gggcactgca
     1861 tcaaaggaag tggtgtatgt ctttgatccg gccacaaacg cagtctgcag tccgggcttt
     1921 tccttcgttc ctgccggggc agcaaagaag tgcgagttgc tgtccttccc gaaatcaaaa
     1981 aacagctgtc ccggttttgc atcgcgaagc cagatccatc cggtgatggt gagcgattcc
     2041 tcgcctgaca ccgcctcgcc agggatggaa acatatgtct cgctttcgcc cgaaagggca
     2101 agaacattac caaagaggct gtcgggtatg aatctgaagt cggttccccg ggtgatggca
     2161 tggaggttgt tgcgcgacca gtccctgtca tttcccttga actggtagcg ggagatcagt
     2221 ccggtttcac ctattccgtc gaggatctgg tcaccgtcct gggcaagcgc agggaatgtt
     2281 gtcaacgcgc ataaaatcaa tacaatacca acgagcagcc tgtcaatttt catgttcagt
     2341 atcttttgtt ctggtttatc cgccaatgca tcagcaagtt taacaaattc tcatgacgaa
     2401 aatgagagaa ttgttaatgc tccgccaaac gaacatacca ttagcaggct ataaatatat
     2461 aaaattggtc agttccagct ttcccagcaa gctgatctct gtcaggttac ggacatcgtt
     2521 gtcagtaaat tccgataacg caccttcttt cttccaggcg cctgagacaa aatagacgaa
     2581 gtcctccttc tgcatccaga ccctggcatt cgggaaaagg tgaattcctc cgatatgatc
     2641 cggatgagga tgtgtaatta ttacatctgt tatacaggaa ggggatacgt tgagcctttt
     2701 cagcacac
//
LOCUS       DAOPXU010000193         2646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_54810_length_2646_cov_9.867619, whole genome shotgun sequence.
ACCESSION   DAOPXU010000193 DAOPXU010000000
VERSION     DAOPXU010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54810_length_2646_cov_9.867619"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PKK03_12280"
     CDS             complement(<1..1033)
                     /locus_tag="PKK03_12280"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNX85217.1"
                     /translation="MKKLIFIFLSVLFVNAGMAQTRVTGTVTASEDGSPIPYAVVLVK
                     GLRGVGTNTDADGKYALSNVPSDAVLVFSYVGYKTQEIQVSGRSVINVEMATDALALD
                     EVIVVAYGTSTKGTFTGAASVVKADAIRDVPTLSFEKAMGGKIAGLQITTLSGQAGST
                     SSIRVRGVGSMNASNEPLYVVDGVPVISGNVTQMGIYTSSNVMNSINPNDIESITVLK
                     DAAASALYGSRAANGVIVITTKQGKQGKPVVNFKASVGITPTFATDNYETASTEQQVE
                     LYYENFWNAAILYDGDNYEDASTSALAQLNKRFNKHGYTFAAPDNTVNSLTIGGERAG
                     RYFDWEDVLF"
     gene            1361..>2646
                     /locus_tag="PKK03_12285"
     CDS             1361..>2646
                     /locus_tag="PKK03_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85218.1"
                     /translation="MKLFKAFLPVMLIFFGCNATDTYDVLIRNGNIADGSGSPAFRGD
                     IGIMSDTIAAIGDLRKAGGKTEIDASGMTVAPGFINMLSWAVESLIEDGRSMGDIVQG
                     VTLEVLGEGDSWGPWSESMKKELKASQGDIKYDIGWTTLGEYLEYLEKKGVSTNIASF
                     VGTATVRIHTVGYDNRPPAAEEMDSMKLLVRQAMEEGAIGVSSALEYVPASFASTEEL
                     TELCRVAAEYDGLYISHIRNEDDTFLEAIDDFLKIPAATGIRSEIYHFKQVGRTNWDK
                     LDPAIAKIDSAREAGLHVTADMYNYPASSTGLGIIMPEWVQEGGFEKWVARLRDPKIR
                     KVIGPEIMKTILRKQGTPEKIIVIGFDTDSLKYLQGKTVAEIAAMRGTSPEETIMDLI
                     IDDGSNVSAVFFSMSEDNLRKEIAIPWMSFGSDGKS"
BASE COUNT          695 a          618 c          689 g          644 t
ORIGIN      
        1 ggaacagcac atcctcccag tcgaagtacc tgccggctct ctccccgccg attgttagtg
       61 aattgactgt gttgtcagga gccgcgaagg tatagccatg cttgttgaac cttttgttca
      121 gctgtgccag ggcgctggta ctggcatcct cataattatc accgtcatag aggattgctg
      181 cattccagaa gttttcataa tatagctcca cctgttgttc agtgctggca gtctcgtagt
      241 tgtcagtggc aaatgtcggg gtaataccca cagatgcctt gaaattgacc accggcttgc
      301 cctgtttccc ctgcttggtg gtgatgacga ttaccccgtt ggctgccctc gaaccgtaca
      361 gggccgaagc agctgcgtcc ttcagaacgg taatcgactc gatgtcattg gggttgatgg
      421 agttcattac attgctggaa gtgtagattc ccatctgtgt gacattacct gatatcacgg
      481 gaacaccatc aacaacataa agaggttcat ttgatgcatt catggagcca acgccgcgca
      541 ccctgattga tgaggtggaa ccggcctggc ctgaaagggt tgtgatctga agacctgcga
      601 tcttgccacc catggccttc tcgaatgaca gggtgggaac atccctgatg gcgtcagctt
      661 ttaccacact ggcggcaccg gtgaaagtgc cctttgtgga tgtaccgtag gcaacaacaa
      721 tcacctcgtc aagcgcaaga gcatcagtgg ccatctcaac attgatgaca gacctccctg
      781 atacctggat ctcctgtgtt ttgtagccga catagctgaa caccagcaca gcatcggaag
      841 gaacattgct gagggcatat tttccgtcag catctgtatt ggttccaact ccccgtagtc
      901 cctttaccag taccactgca taggggatcg gagaaccatc ttcagaggcg gttaccgttc
      961 cggtaacccg tgtctgcgcc atgccggcat tgacaaacaa taccgacagg aaaatgaaga
     1021 ttagtttttt catgggaaat tagcgttagt taataaaggt taactctcac aagttacaga
     1081 gaaatttatt ttataaaaaa aaatttgtat tacatcatac tgttttaaag catagaggtg
     1141 aagtaacatg gttaatgtac tgaaaaacac atcagatgcc agcacgaggc ttccggatgt
     1201 catccggaag attccgggcg gaaaccatcc tctctggaaa tttatgtcct acataatctc
     1261 ggaagaggga aaatttgcat tcatgctgca tgaaaattta tcgcgcaatc atattatttc
     1321 gttcgctttt gagtaaattt cactcaaatc tttgctcatc atgaaacttt tcaaagcttt
     1381 tttgccggta atgctgatat ttttcggctg taatgccact gacacgtatg atgtactcat
     1441 caggaacgga aatattgcag acggatcagg ctctccggcc ttcaggggtg atattggtat
     1501 tatgtctgac accattgctg ccattggtga cctgcggaag gccgggggta aaacagagat
     1561 agatgcctcc gggatgaccg ttgcgccagg gtttattaac atgctcagct gggcagtgga
     1621 atcacttatt gaggatggca ggtcaatggg cgacatagtt cagggggtga ccctcgaggt
     1681 gctcggtgag ggtgactctt ggggcccctg gagcgaaagc atgaagaagg aattaaaggc
     1741 atcacagggc gacataaaat atgacatagg atggacgacg ctgggtgaat acctcgaata
     1801 cctcgaaaag aagggggtct caaccaatat cgcatctttt gtggggactg caaccgtcag
     1861 gatacatact gttggatatg acaaccgtcc gcctgctgct gaagagatgg attcgatgaa
     1921 gctgctcgta aggcaggcta tggaagaagg cgccattggt gtcagctcgg ccctggaata
     1981 tgtcccggcc agcttcgcct ccaccgaaga gctgaccgaa ctctgccggg tggctgcgga
     2041 gtatgacgga ttgtatatct cacatatcag gaacgaggat gacaccttcc tggaagccat
     2101 tgatgatttc ctgaagattc ctgctgcaac aggcatcagg tctgagatat accacttcaa
     2161 gcaggtgggg cggaccaact gggacaaact tgacccggct attgcaaaaa tagattcggc
     2221 acgggaggca gggctccatg tcacagcaga catgtacaat tacccggcaa gctcaaccgg
     2281 acttggtatc atcatgcccg aatgggtgca ggaaggcggg tttgaaaagt gggtcgcaag
     2341 gctccgtgat ccgaagataa gaaaggtgat cgggccggag ataatgaaga caatcctgag
     2401 aaaacagggt acacccgaaa agattattgt catcggtttc gataccgact cactgaaata
     2461 cctgcaggga aaaacggttg ccgaaatagc tgccatgagg ggcacatcac cggaggagac
     2521 catcatggac ctcatcatcg atgacggaag caatgtctcg gccgttttct tctcaatgtc
     2581 agaggataac ctcaggaagg agatagccat tccctggatg agcttcggct cagacggcaa
     2641 atcgat
//
LOCUS       DAOPXU010000194         2619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_55647_length_2619_cov_17.774571, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000194 DAOPXU010000000
VERSION     DAOPXU010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55647_length_2619_cov_17.774571"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1883)
                     /gene="recJ"
                     /locus_tag="PKK03_12290"
     CDS             complement(177..1883)
                     /gene="recJ"
                     /locus_tag="PKK03_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNX85219.1"
                     /translation="MEKRWLLKERGDTRVVTTLATEMAVPAAVASLMAQRGITTKSQA
                     EEFFNPTLGSLHDPFLMKDMNRAVDRISTAISRNEKILVYGDYDVDGTTSVAMLYSFL
                     RERHSSLDYYIPDRYHEGYGLSLKGIDYAAEGNSRLIIALDCGIKAVDKVKYARSKGI
                     DIIICDHHLPGDGIPDATAVLDPKQPGCGYPYKELSGCGVGFKLMQAICKVQNIPFER
                     IIPYLDMVAVSIGSDIVPMTGENRVLAYYGLRQLNESPRLGFQKIIEKAKIRKPLAIE
                     DVVFRIGPRINAAGRMESGSRAVDLLVSQNPQEALTICDAIDINNDDRRKKDSEITSE
                     ARRMVSEDSRSGNTRTTVLFHQGWHKGVIGIVASRLIETYYRPTVILTESKDGFATGS
                     ARSVPGYDLYQAIESCSDLLESFGGHMFAAGLTLKQENLQQFRERFEKFVSETIRDEH
                     LVPSVNVDEEIPLEEVTMELYRNLEKFQPFGPENTAPVFVSRRVKTLSCQPVGTNGMH
                     LKLTFARQNGEPVGAIAFGQADMIETFRESGGVVDIAYSVELNEFRGKTSVQLNIKDI
                     KG"
     gene            complement(1895..2587)
                     /gene="lipB"
                     /locus_tag="PKK03_12295"
     CDS             complement(1895..2587)
                     /gene="lipB"
                     /locus_tag="PKK03_12295"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNX85220.1"
                     /translation="MPNLVIYDDLGLRDYKETWDYQESLFNAKVEEKRSPAADGAKTP
                     DHLIFVEHNHVYTLGKSGSEQNLLLDYIQLQARDASFYRIDRGGDITYHGPGQLVGYP
                     IFDLEEMKLGLRDYIHLLEEAIIMCISRFGIEGSRLEGATGVWIDPDKGARARKMCAI
                     GVRASRFVTMHGFALNVSTDLSYFNHINPCGFTDKGVTSIEKETGRKITMEDVKRVMY
                     NTMREVFGYEYY"
BASE COUNT          596 a          741 c          619 g          663 t
ORIGIN      
        1 ttccaccgca gcatcatggt gagacataat tactccatca aatttaggac aggaaagcga
       61 gaatgtcaag ggaaagatga taaaaaaagg cagcaggcag taggcagaag gcagtagtgt
      121 gacagcgttg gcaataacat gctgatttta tgactattgc ctaatgccta tcgcctctac
      181 cctttaatat cctttatatt gagctgtact gacgtcttcc cgcgaaactc attcagctcc
      241 actgagtagg ctatgtcaac aacaccgcct gactcacgga aggtctcgat catgtcagcc
      301 tggccaaacg caatggcacc cacaggctca ccattctgcc ttgcaaaggt gagcttgaga
      361 tgcatcccgt ttgtgcccac aggctggcat gagagggtct tgaccctcct cgagacaaac
      421 accggcgctg tgttctcagg accgaacggc tgaaacttct caagattccg gtaaagttcc
      481 atggtgacct cttcaagagg aatctcctca tcgacattga ctgacggcac caggtgctca
      541 tcccttatcg tctcgctgac aaatttctca aacctctcgc gaaactgctg caggttctcc
      601 tgcttcaggg tcagtccggc cgcaaacata tgccctccaa aactctcgag cagatcagag
      661 caggactcga tggcctggta aaggtcatac cccggcacac tgcgtgcaga gccggtggca
      721 aaaccatcct tcgactccgt cagtatcacc gtaggcctgt aatatgtctc aataaggcgt
      781 gatgccacaa taccgatcac ccccttgtgc catccctggt gaaacagcac cgtagtgcgg
      841 gtattgcctg acctgctgtc ctcgctcacc atacggcgcg cttcggatgt gatctcactg
      901 tcttttttcc tgcggtcgtc gttattgatg tcaattgcat cgcagattgt cagtgcctcc
      961 tgcggattct gcgataccag cagatcgacc gccctgctgc ctgactccat ccgtccggcg
     1021 gcgtttatgc ggggcccgat gcgaaacacc acatcctcaa tggcaagcgg tttgcggatc
     1081 ttggcctttt caattatttt ctgaaatccc aggcggggcg attcgttgag ctgcctcagc
     1141 ccatagtaag ccagcaccct gttttcgccg gtcataggca caatgtctga cccgatactt
     1201 accgccacca tatccaggta aggaatgatc ctctcaaacg ggatgttctg caccttgcat
     1261 atggcctgca tcagtttgaa tcccacaccg caccctgaca gctccttgta gggataaccg
     1321 caccccggct gcttggggtc aagtacagca gtggcatcag ggatcccgtc gcccgggagg
     1381 tgatggtcac aaataatgat atctataccc tttgaccgtg cgtatttcac cttgtcaaca
     1441 gccttaattc cgcagtcgag cgcaattatc agcctgctgt taccctcagc ggcatagtca
     1501 atacccttga gggaaagccc gtacccctca tggtaacggt cagggatgta gtaatcaagg
     1561 ctagaatgtc tctccctcag gaaggaatag agcattgcca ccgaggtggt gccgtcaaca
     1621 tcatagtcgc cgtaaacaag tatcttttcg ttccttgata ttgccgtgct gatcctgtca
     1681 accgccctgt tcatgtcctt catcaggaag gggtcatgca gggatcccag tgtggggttg
     1741 aagaactcct cagcctggct ttttgtcgtt atgccccgct gggccatcag gctggcgaca
     1801 gcggcaggca ccgccatctc tgtcgccagg gtcgtgacga cacgtgtgtc gcccctctct
     1861 ttcaagagcc accttttttc cattatattt atttttaata atattcatat ccgaagacct
     1921 cccgcattgt gttgtacatc actcgcttca catcctccat tgtaatcttt cgccccgtct
     1981 ctttctcaat gcttgtaaca cctttgtcgg tgaagccgca cgggttgata tggttaaaat
     2041 aggagaggtc agtgctgacg ttaagggcaa agccgtgcat cgtcacgaac cgcgatgctc
     2101 ttacgccaat tgcacacatc tttcttgcgc gggctccctt gtcggggtca atccataccc
     2161 ccgtggcgcc ctccaggcgg ctcccttcta taccgaaacg ggagatgcac atgataattg
     2221 cttcttcaag cagatgaata taatcgcgaa gcccaagctt catttcttca aggtcaaaga
     2281 tcgggtaacc gacgagctgg ccgggaccgt gataggtaat gtcacctccc ctgtcaatcc
     2341 tgtagaacga agcatcgcgg gcctgcaact ggatgtagtc aagcaacagg ttctgctccg
     2401 agccgctctt gccaagggta tacacatgat tgtgttcaac aaagatcaga tggtcaggtg
     2461 tcttagcccc gtcagcagca ggcgatctct tctcttctac cttggcattg aagaggctct
     2521 cctggtaatc ccaggtctcc ttataatccc tgaggccaag atcatcatat ataaccaggt
     2581 tgggcatgtt tatgttgtcg gttttccgtt atcggttat
//
LOCUS       DAOPXU010000195         2610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_55945_length_2610_cov_11.895499, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000195 DAOPXU010000000
VERSION     DAOPXU010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55945_length_2610_cov_11.895499"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="PKK03_12300"
     CDS             complement(<1..1319)
                     /locus_tag="PKK03_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HNX85221.1"
                     /translation="MVTVIIPALNEAETIGTVIGLVKRSPLVSDIIVMDDKSHDNTIT
                     VAREAGATVFTSTSLGKGASMREGILFAKNEFLLFLDADITTYPENIVELMTGPLADG
                     TADFVKSVFTRQAGRVTELVAKPLLRLLFPDLARFAQPLSGMVAGKKSMLQNLELEND
                     YGVDIGMLIDMHIMGVRISEVNIGYIENRMQSWEQLSKMSLEVSRAIIRRGEPLQPKE
                     GETLKKVNVLRTQMEYAVRENRQGFSRMVIFDMDNTLLKGSFITTASRILGFEEELIN
                     IVTHNDTAIMRTKLIATLMKGHTVKEVLDVVDTIPMVEDALEVVTELKKNGYICGIIS
                     DSYDVVTNHIKNKLGMDFSVANELEFSNSVATGEVKIPSYFLRTKQSQCSHEYCKSNV
                     IRQLSASYGISISNITAIGDSDNDVCMVRECGTGISFYSQNKYLNLVA"
     gene            complement(1313..1507)
                     /locus_tag="PKK03_12305"
     CDS             complement(1313..1507)
                     /locus_tag="PKK03_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85222.1"
                     /translation="MSGRLICTKLAGILSLVTLSGSAQTGKDTLRVLFVGNSYTCYEN
                     LPQVVMETVGSKVTSEAMPW"
     gene            complement(1510..2229)
                     /locus_tag="PKK03_12310"
     CDS             complement(1510..2229)
                     /locus_tag="PKK03_12310"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX85223.1"
                     /translation="MKHEYYLSVMIQIPDTEKGTRIMRLTPARCNIWVVTRNDRTIIV
                     DTGISFDRSRIEHALERKGLIPEAVILTHTHFDHAGNAAWLQAEYGAEVVVQEAESAM
                     LATGDMPIPNGTYTFTRGLVGIGRKMQPVFRYDPCVAEHVFTDQFDLNRFGINGFIIH
                     TPGHSPGGATVVIDDEIAIAGDSVIGTMPGSPFPPFADDVPLLLTSWKKIIGTGCRSF
                     LPGHGKQVSREEMINEYEKRK"
     gene            2286..>2610
                     /locus_tag="PKK03_12315"
     CDS             2286..>2610
                     /locus_tag="PKK03_12315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HNX85224.1"
                     /translation="MKPIHIYQVDAFTHGPFTGNPAAVCVLTDPVTEETMQKIAMENN
                     LSETAFITRGNDEWLIRWFTPMEEVDLCGHATLASAFVVLNIIDPQSETVVFRSRVMG
                     LLTVRK"
BASE COUNT          600 a          724 c          600 g          686 t
ORIGIN      
        1 gccacgaggt tcaggtactt gttctgcgaa taaaacgaga tgcctgtgcc gcactcgcgt
       61 accatgcaga catcattgtc actgtcgcct atggcggtga tgttgctgat gctgatgcca
      121 taactggccg aaagctgcct tatcacgttt gacttgcaat actcatggct gcactggctc
      181 tgcttcgtcc ttaagaaata tgaaggaatt ttcacctcgc cagtggccac gctgtttgag
      241 aactcaagct cgtttgccac ggagaagtcc atcccgagct tattcttgat atgatttgtg
      301 accacatcgt aactgtcgct gatgataccg cagatgtaac cgttcttttt cagttcggta
      361 accacttcaa gagcatcctc caccatcggg attgtatcta ctacgtcaag tacctcctta
      421 accgtatgac ctttcatcag ggtggcgatg agtttggttc tcattatggc agtatcattg
      481 tgggtcacta tgttgatcag ttcctcctca aatcccagta tccgtgatgc agtggttatg
      541 aaactcccct tcagcagtgt gttgtccatg tcaaagatca ccattcggga gaatccctgc
      601 cggttttcgc ggacagcata ctccatctgt gtcctgagta cgttcacctt tttcagcgtc
      661 tctccctctt ttggctgcag cggctcaccc cggcggatta tagccctgga cacctcgagg
      721 gacatcttcg agagctgctc ccatgactgc atccggtttt ctatgtatcc tatgtttact
      781 tcggagatcc tgacgcccat aatgtgcatg tcaatcagca ttcctatatc gacgccatag
      841 tcattctcca gttccaggtt ctgcagcatt gactttttgc cggctaccat accgctcaga
      901 ggctgggcaa acctggccag gtcagggaac agcagcctta gcagcggctt tgccaccagc
      961 tcggtgaccc ttccggcctg ccgggtgaat accgatttga cgaagtcggc cgtaccgtca
     1021 gcaagcggtc cggtcatcag ttcgacgata ttttccggat aggtggttat gtctgcatcc
     1081 agaaacagca aaaactcatt ctttgcaaac agtatccctt cgcgcatcga agcgcccttg
     1141 ccaagggagg tgcttgtaaa gacagttgct cccgcctcgc gtgctacagt aatggtatta
     1201 tcatgggact tgtcatccat gacaattata tctgaaacca aaggtgacct tttcaccagc
     1261 cctatgacgg tgccaatggt ttctgcctca ttcagcgccg ggattattac tgttaccatg
     1321 gcattgcctc tgacgtcact ttgcttccga cagtctccat taccacctgc ggaaggtttt
     1381 cataacaggt gtagctgttg cccacaaaca gaaccctgag cgtgtccttc ccggtctgtg
     1441 cagagccgct gagagtcacc aggctcaata tcccggcaag tttggtacaa attaatcttc
     1501 ctgacatctt tattttcttt tctcgtattc atttatcatc tcctccctgc ttacctgctt
     1561 gccgtgaccg ggaaggaagg atcggcagcc ggtgccgatg atcttcttcc agcttgtcag
     1621 aagcaatggg acatcatctg caaatggcgg gaatggcgag ccgggcattg tcccgattac
     1681 cgagtcgccc gcaatagcta tctcatcatc aatcaccacc gtagcccctc ccggtgagtg
     1741 acccggcgta tgtataataa acccgtttat cccaaaacgg ttcaaatcaa actgatcagt
     1801 aaagacatgt tcggcgacac acggatcata cctgaaaacc ggctgcatct tcctgccaat
     1861 ccccaccagg ccccgggtga aggtgtaggt cccgttcggt atcggcatat ccccggtggc
     1921 aagcatggcg ctctcggcct cctgcaccac cacctcggca ccatattccg cctgcagcca
     1981 ggctgcattg ccggcatggt caaaatgggt gtgtgtgagg atcaccgctt cggggatcag
     2041 cccctttcgc tccagcgcat gttctatccg gctcctgtca aaagagatgc ctgtatcaac
     2101 aataattgtc cggtcgttcc gcgtaacaac ccatatattg cacctggcag gcgtcagcct
     2161 catgatcctg gtgcccttct ccgtatcagg aatctgaatc atgacactaa gataatactc
     2221 atgcttcatc cattgtcaat ttgtgataaa caatggtaac tttgggactg caaaaatgat
     2281 cttaaatgaa gcccatacac atttaccagg ttgatgcatt cactcacgga cccttcaccg
     2341 gcaaccctgc tgcagtatgc gttctgactg acccggtgac agaagaaaca atgcagaaga
     2401 tagcgatgga aaacaacctc tctgaaactg catttatcac acgcggaaat gatgaatggc
     2461 tgataagatg gttcacaccg atggaagaag ttgacctgtg cggccacgca acccttgcct
     2521 cggcatttgt ggttctgaat atcattgatc cgcagagcga aacggtagta ttccgctcca
     2581 gggtgatggg acttctcact gtcaggaaaa
//
LOCUS       DAOPXU010000196         2595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_56432_length_2595_cov_10.583858, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000196 DAOPXU010000000
VERSION     DAOPXU010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56432_length_2595_cov_10.583858"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /gene="rlmD"
                     /locus_tag="PKK03_12320"
     CDS             <1..1401
                     /gene="rlmD"
                     /locus_tag="PKK03_12320"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNX85225.1"
                     /translation="QLPILRGVTITDIGSEGNAIAKVDDMVLFVPGMVPGDVVDVRVT
                     KKKKRYMEGVALEVITPSPGRAEPPCRHFGVCGGCRWQHLPYDRQLFYKAKQVTDNLT
                     RIGHINIGQVDPILGSEKIYGYRNKLEFTFSKRRWMTREEIGTEAVVTEPALGFHIPG
                     IFDKVLDIKECLLMSEPADTIRNEVRKYALEKRLPFYDFREHSGFLRNMIVRKSSTGE
                     VMVVLVTTDALPGERDLLLGFLTERFPEITSLYSIINTKRNDSISDLQPVHMAGSMFI
                     TEVMDGLRFRVGPKSFYQTNSGQSVRLYGIVKEFAQLTGRENVYDLYTGTGTIACYLA
                     SQAEGVTGIEYIEEAVADARVNAVENGISNAFFIAGDIKDTLTDELFLRRGKPDVIIT
                     DPPRAGMHADVVNAMLRSGADRIVYVSCNPATQARDIGILSAVYDVARITPVDMFPHT
                     FHVENVALLVKNARTG"
     gene            1544..1630
                     /locus_tag="PKK03_12325"
     tRNA            1544..1630
                     /locus_tag="PKK03_12325"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1578..1580,aa:Ser,seq:gct)
     gene            1841..>2595
                     /locus_tag="PKK03_12330"
     CDS             1841..>2595
                     /locus_tag="PKK03_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85226.1"
                     /translation="MKRMLSSIFSGLYSLETRSFLLFFLLCKYRFRHFTEPEISRYQA
                     RQIRQITKYAIRHSQFFHNHYKDCDLTYFTNLPTVNKKIMMDNLTDYNTVGLTREEII
                     NFCLEVEKSRDFSRRLNGLNVGMSSGTSGNKGVEILTRREEMYMKAALFARFHFPRKE
                     KLNLAFILRVSSPAFNLNILGHRMTYISQLDSTEEVNDQLQRLQPNVISAPPSMLKII
                     AREVEAGRLSVSPERLVSYAEILYPDVKKYLSDV"
BASE COUNT          652 a          624 c          715 g          604 t
ORIGIN      
        1 cagttgccca tactaagggg cgtcacgatt acggatatag gctcagaagg caacgctatc
       61 gcgaaggtcg atgacatggt gctcttcgtg ccgggaatgg ttcccggcga tgtggttgat
      121 gtccgtgtca caaaaaagaa gaagaggtac atggaagggg ttgcgctgga ggttattacc
      181 ccttcgcccg gcagggcaga accgccatgc aggcatttcg gggtgtgtgg aggatgccgt
      241 tggcagcatc ttccgtatga ccggcagctt ttttacaagg cgaaacaggt gacagacaac
      301 ctgacgcgga ttgggcatat taatatcggg caggttgacc cgatccttgg ttctgagaag
      361 atttacggct accggaacaa gcttgaattc actttttcca agaggcgatg gatgacacgg
      421 gaggagatcg ggacagaggc agtagtgact gagcctgccc tcggatttca catcccggga
      481 atctttgaca aggtgcttga catcaaagaa tgcctcctga tgtcagagcc ggcagatact
      541 atccgtaatg aggtcagaaa atacgcgctc gagaagcggc tgcccttcta tgatttcagg
      601 gaacacagcg gattcctccg caacatgatc gtcaggaaat cctcaacagg tgaggttatg
      661 gtcgtcctcg tgacaaccga tgccttaccc ggtgagcggg acctgctgct tgggttcctg
      721 accgaaaggt tcccggaaat cacttcgctg tatagcataa taaataccaa gaggaacgat
      781 agtatctccg acctgcagcc ggttcatatg gccggaagca tgttcatcac agaggtgatg
      841 gacggacttc gattcagggt tggcccgaag tcgttttacc agacaaacag cggccagtcg
      901 gtgaggttgt acgggattgt gaaagagttc gcgcagttga ccggcaggga gaatgtttat
      961 gacctgtata ccggtaccgg cactatcgcg tgttacctgg catcgcaggc tgagggggtg
     1021 acggggatag agtatattga ggaggcggtg gctgacgccc gggtcaacgc tgttgagaac
     1081 ggaatttcga acgccttttt cattgcgggc gacataaagg atacccttac tgatgagctt
     1141 ttcctcaggc gcgggaagcc tgatgttata atcactgatc cgccacgggc cggcatgcac
     1201 gccgatgtgg taaacgctat gctgcgctcg ggcgctgaca ggatagtgta tgtcagctgt
     1261 aatccggcca cacaggcacg cgacatcggg atcctttcag cagtgtacga tgtggcgcgc
     1321 atcaccccgg tcgatatgtt cccgcatacc ttccatgtgg agaatgtggc gctgctggtg
     1381 aaaaatgccc ggactggtta agtcagaata atgtaaacag tcatgagttt aaaagagaat
     1441 cagaattttg taaagtatcc aatgttttta tatttttgcc cctctgttga aaaccgggca
     1501 gattacattt accggtgaac aacaagacaa aatagtgttt tttggagcga tgggtgagag
     1561 gcttaaacca gcagtttgct aaactgccgt acgggtaacc gtaccggggg ttcgaatccc
     1621 cctctctccg ctgaagtcaa agcaggccgg gatacagata gtaaccggcc tttttttttt
     1681 gagctggccc ggatgagcca acacataaat gctgaccatg atatcaggaa gacatgcggg
     1741 tattcaagtc ctccccgggc aaccattgac aatgcccttg atgaattcct atatttgcat
     1801 taatactgtc tggaaggggg agtgctaatg accggttctg atgaaaagga tgctctcttc
     1861 catattcagc ggactgtaca gtctcgaaac caggagtttc ctcctgttct tcctcctctg
     1921 caaataccgg ttccgccatt tcacagagcc ggagatcagc cggtaccagg ccagacagat
     1981 ccgccagatc acaaaatacg ctatcagaca ttcgcagttt ttccacaatc actacaagga
     2041 ctgtgacctg acctatttca caaatctccc cactgtcaac aagaaaatca tgatggacaa
     2101 cctgactgac tacaatacag tcggattgac ccgggaggag ataataaact tttgcctgga
     2161 agttgagaaa tcgagggact tttcccgccg actgaacgga ctgaatgttg gcatgtcgtc
     2221 cggcaccagc ggcaacaagg gagtggagat cctgacaagg cgcgaggaga tgtacatgaa
     2281 ggcggccctg tttgcccgtt ttcatttccc gaggaaggag aagctgaacc tcgcatttat
     2341 cctcagggtg agctcccctg ctttcaacct gaatattctg ggccacagga tgacttacat
     2401 aagccagttg gattcgacgg aggaggtgaa tgatcagttg cagcggcttc agccgaacgt
     2461 catctcggca ccgccatcga tgcttaagat aatcgccagg gaagtcgaag ccggaagact
     2521 ttcggtctcg ccggagcggt tagtctccta tgcggagatc ctttatcccg acgtgaaaaa
     2581 gtacctgtcc gacgt
//
LOCUS       DAOPXU010000197         2578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_57010_length_2578_cov_8.003171, whole genome shotgun sequence.
ACCESSION   DAOPXU010000197 DAOPXU010000000
VERSION     DAOPXU010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57010_length_2578_cov_8.003171"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PKK03_12335"
     CDS             complement(<1..324)
                     /locus_tag="PKK03_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85227.1"
                     /translation="MKRVVIILLLGFLHAGVYGQRNDTVVADTVVADTVMAPVHVLKT
                     ATFDGQTYPFVELKEIIVIGKMPRGVRFDYRRHARLVYNVRRVYPFALIVRDEFGRIN
                     RLLETL"
     gene            430..1068
                     /locus_tag="PKK03_12340"
     CDS             430..1068
                     /locus_tag="PKK03_12340"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HNX85228.1"
                     /translation="MKHTPDTDQLDDPSLYEKVLTLPYDEIGPFVLGEMTRYTRPMLR
                     IWITFAATIFLSVWFWPGVAYHPEEPRIVAGLAAGLILIPLLLVPVHEGSHLIPFLLA
                     GARDIRFGADLRQGIIYVTAHRFVADKRLFSLVASTPFILITVAILAAILLSPPWWKW
                     VLSMTLLVHTTMCAGDAALTGFMSRFGKRRVYTWDDADRKEAYFYASAETIG"
     gene            1128..2570
                     /locus_tag="PKK03_12345"
     CDS             1128..2570
                     /locus_tag="PKK03_12345"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX85229.1"
                     /translation="MSSFTQSLKKFPRLFWISNIIELFERWGWYAFYNGFIALYLTTP
                     KEDGALGFTNVEKGTIMGTASMILYFLPVITGAVADRVGYKKVLITSFITYVISFFML
                     SRFETFGSIFAAFILLAVAGALFKPLISGTVARVTDRETASLGFGIFYMVVNIGGFIG
                     PFVASLLYRNNWDSVFYMSIAAMTLNLLLTIFFYKEPAVTGNGNSFVKNIGIAFRNIG
                     VTLTDWRFLLFLLIIGVFWTAYNQLYYSFPVFLESWVNLDGMSASLGLKPGTITTVTI
                     SSLASFFIILFQLIISSITARLRPLNSIMTGIFILAFGLGMMFANLNPWIIVLGVLIF
                     GIGEMASSPKVQEYLGGIAPPDRKALYMGTAFLPIALGHLGAGWISGKPYEVRADKLF
                     LLKKAVEEKGFDIPEISGSFTQTDYFNRAQELFGMDSHQLTRYLWDTYSPSRVWILFT
                     AIAIAASVLLYLYDRLILKGRDAAGNGSRQ"
BASE COUNT          627 a          701 c          605 g          645 t
ORIGIN      
        1 aagagtctca agaagcctgt ttatccggcc gaactcatcc ctgactatca gcgcaaacgg
       61 ataaacacga cgcacattat aaacaagacg ggcatgcctg cggtaatcaa acctgactcc
      121 tcttggcatc tttccgatga caataatctc tttcagctcc acaaacggat aagtctgccc
      181 gtcaaatgtc gcagtcttaa gcacatgcac cggcgccatg acagtgtcag ccacaacagt
      241 gtcagccaca acagtgtcat tcctctgtcc gtaaacgccc gcatgaagaa aaccaaggag
      301 cagtatgata accactcttt tcataattac ctgcaaattt aaccaattgt cgtgccaaag
      361 ggttcacggg aaggtaaaag tgagcattgt cattatgtat aaacagctat cagctatctt
      421 tgggaactca tgaaacatac accggataca gaccaacttg atgacccttc attgtatgag
      481 aaggtactga cccttccgta cgatgagatc ggtccttttg tgctcgggga gatgaccagg
      541 tataccaggc cgatgctccg tatctggatc acctttgctg caaccatctt tctgtcagta
      601 tggttctggc cgggagtggc ataccacccg gaagagccgc ggattgtcgc cggacttgct
      661 gccggactga tactgatccc gctgttgctg gtcccggtgc atgagggttc gcacctgata
      721 cccttcctcc ttgcaggagc ccgcgacata agattcgggg cagacctgcg ccagggaatc
      781 atttacgtaa cggcgcacag attcgtcgca gataagagac tcttctcgct ggttgcatcc
      841 acgcccttca tcctgattac agtcgctatc ctggcagcca tcctcctctc tccaccctgg
      901 tggaaatggg tgctgtcgat gaccctgctg gtacatacca ccatgtgcgc cggtgatgcc
      961 gcgctgaccg gcttcatgag caggttcggc aagaggaggg tatatacatg ggatgacgca
     1021 gacaggaagg aggcttattt ctatgcctcc gcagaaacca tcggttaaag aacaactgta
     1081 agacagtaat acatatttca taaatttaaa cccgcaaaac aaacctgatg agcagcttta
     1141 cccagtcatt gaaaaaattc ccgcgattat tctggatatc aaacatcatt gagctgttcg
     1201 aacggtgggg ctggtatgcc ttctacaacg gattcatagc gctttacctc accactccta
     1261 aagaggacgg agccctgggg ttcacaaacg tggaaaaggg aaccataatg ggtaccgcct
     1321 caatgattct gtatttcctt cccgttatca ccggtgccgt tgccgacagg gtcgggtaca
     1381 aaaaggtgct gattacttca ttcatcacct atgtcatctc attcttcatg ctgagccggt
     1441 tcgaaacatt cggttccatt ttcgccgcct tcatcctcct ggctgtagca ggcgctctct
     1501 tcaaaccgct tatatccggg accgtggccc gcgtcactga cagggagaca gcatctctcg
     1561 gattcggaat attctatatg gtggtgaata tcggcggctt catcggcccg tttgtcgcca
     1621 gcctgcttta caggaacaac tgggactcgg tgttctatat gtctatcgca gccatgaccc
     1681 tcaacctcct tctcaccatc ttcttctaca aggaacctgc cgtgacaggg aacgggaaca
     1741 gtttcgtcaa aaatataggg atagcattca gaaatatcgg ggtaaccctt accgactggc
     1801 gtttcctgct ctttctgctt atcataggtg tcttctggac ggcttataac cagctttatt
     1861 actcattccc cgtcttcctg gagtcatggg tgaacctcga cgggatgtca gcctccctgg
     1921 gacttaaacc cggaaccata accacagtca caatctcaag cctcgcgtca ttctttatca
     1981 ttctcttcca gctgattatc tcatcgataa cggccaggct caggcccctg aacagtatca
     2041 tgaccgggat ctttatcctg gctttcggac tgggaatgat gtttgcaaac ctcaatccct
     2101 ggataatagt ccttggcgtt ctgattttcg gcatcggcga gatggcttct tcacccaagg
     2161 tgcaggaata tctcggcgga attgctccgc ctgacagaaa ggctctctac atgggtactg
     2221 ccttcctgcc gatagccctt ggccacctgg gtgccggatg gatatccggc aaaccatacg
     2281 aggtaagggc tgacaaactg ttcctcctga aaaaggcagt ggaggaaaag ggatttgata
     2341 tccctgaaat ttccggaagc ttcacccaga ccgactactt taacagggca caggagctct
     2401 tcggaatgga ctcgcatcaa ctgacacgat acctgtggga tacatatagc ccctcgcgtg
     2461 tctggatact ctttactgcc atcgccattg ccgccagtgt tctgctttac ctttatgacc
     2521 gccttatcct gaaaggacgg gacgcggcag ggaacggatc caggcagtag gtattagg
//
LOCUS       DAOPXU010000198         2537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_58424_length_2537_cov_15.391217, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000198 DAOPXU010000000
VERSION     DAOPXU010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58424_length_2537_cov_15.391217"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2537
                     /locus_tag="PKK03_12350"
     CDS             <1..>2537
                     /locus_tag="PKK03_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX85230.1"
                     /translation="GIAIAIGVMVDIGIVDVENILRHLEMPENRGIKGKKLMRVIYEA
                     TTEVRAAVVTSIATTIVSFLPVFAMQAQEGKLFHPLAWTKTFALLSAFVLGIVVLPTL
                     VHIFFNISFDRRKIKIAWNSVLIIAGITLAIVFKSWPAIALVAVGVNNLLDHKWSVHR
                     KEYPNYINILITVLFATWYLSIEWLPLGAHNSEFANFLFVSGIVTVILLALMSMVRYY
                     EKILRWALENKRKFLLIPAVTLFFGLLVWQGADRILGFIPGGAEKLGWKNIRQTSFWQ
                     KTTAAFPGAGKEFMPSLNEGSFLLMPTSMPHSGIQMNLEYIETLDRRLSAIPEVEIAV
                     GKWGRVSSALDPAPVQMFENTINYRSEYILDENGHRMRFKVNKNGDYILAGEKTYNPG
                     TQDFRVIPADSLIPDRKGEYFRQWRPHIKRPDDIWQEIVKVTDIPGLTSAPKLQPIET
                     RLVMLSTGMRAPMGLKVYGPDLNSIEEAGLIFEKALKEVPSVKGSSVFYDRAVGAPYL
                     GINLNREAMARYGMSVSEVQEILQVAVGGMTLSTSVEGRERFPLRVRYARELRDNPED
                     LKKILVPSMNGAQVPLGSIAEIEYTRGAQMIRSENTFLVGYVIFDKTEGTAEVDVVKQ
                     ADKILKDKINSGEISLPAGVTFKFAGNYEHQLRATKRLALVIPVSLILILLLLYFQFR
                     TITASFIHFSGVFVAFSGGFIMLWLYGQDWFMNFSVAGLNLRDMFQMHEINLSVAVWV
                     GFIALFGIATNDGVIMGTYIHQVFEDLKPKTVHDVREAVVMAGKKRVRPAMMTTAVAV
                     IALLPVLSSTGKGADIMVPMAIPTFGGMIIQVMTIFVVPVFQAIWREG"
BASE COUNT          660 a          559 c          666 g          652 t
ORIGIN      
        1 ggtattgcca tcgccatcgg agtaatggta gacatcggga tcgttgatgt tgagaatatt
       61 ctccggcacc tcgagatgcc tgaaaaccgg ggaataaaag gcaagaaact tatgcgggtt
      121 atctatgagg ccacaacgga ggtcagggca gcagttgtta cctccattgc aactacaata
      181 gtcagcttcc tgcctgtatt tgcgatgcag gcacaggaag gaaaactgtt tcacccgctt
      241 gcatggacca aaacatttgc cctgctgtct gcatttgtcc ttggtattgt tgtgctgcca
      301 acactggttc atatcttctt caatatctca tttgaccgga ggaaaataaa gattgcgtgg
      361 aactctgttc tgataattgc cggcattaca ctagccatag tctttaaatc atggccggcc
      421 attgcattgg ttgccgtagg tgtcaataat cttctggatc acaaatggtc agtccataga
      481 aaagagtatc cgaattacat aaatatttta atcacggttt tgtttgctac ctggtacctt
      541 tcaatagaat ggctgccttt gggggcccat aacagtgagt ttgcgaattt cctgttcgtt
      601 tcagggattg tgacagttat tctacttgca ctgatgtcga tggtccggta ttatgagaaa
      661 attctcaggt gggcgctgga gaacaagcgt aagttcctcc tgatccctgc agtaactctc
      721 tttttcggtc tccttgtctg gcagggtgct gacaggattc tagggtttat tcccggcgga
      781 gccgaaaagc ttgggtggaa gaacatccgg cagacctcgt tctggcaaaa gacaacggcc
      841 gccttccccg gtgccgggaa agagttcatg ccgtcactga atgaaggctc atttctcctg
      901 atgcccacca gcatgcctca ttccggcatc cagatgaacc tcgaatatat tgagacactt
      961 gacagacggt tatctgcaat cccggaagtc gaaattgctg taggcaagtg gggaagggtc
     1021 agctcagctc ttgatccggc gcctgttcag atgtttgaaa acactattaa ctaccggtct
     1081 gaatacattc tcgatgagaa cggacacagg atgaggttta aagtgaataa aaacggagac
     1141 tacatacttg ccggtgaaaa aacatataac ccgggaacac aggacttcag ggtgattccg
     1201 gcagacagcc ttatccccga caggaagggg gagtatttca ggcaatggcg cccgcacata
     1261 aagaggccgg atgatatatg gcaggagatt gtaaaggtta ccgacattcc ggggctgaca
     1321 tctgccccga aacttcagcc gatcgagaca aggcttgtaa tgctttcaac cggcatgcgc
     1381 gccccgatgg gccttaaggt ctacgggccg gatctgaatt caatcgaaga ggccggactg
     1441 atattcgaaa aggcactgaa agaagttccg tctgttaaag gctcctccgt attctatgac
     1501 agggctgtcg gtgctccgta cctcgggatt aatctgaacc gtgaggcaat ggcccggtac
     1561 ggaatgtctg tcagcgaggt acaggaaata cttcaggtcg ccgtgggcgg aatgactctt
     1621 tcaacctcgg ttgagggcag ggaaagattc ccgctcagag tacgctatgc acgggaactc
     1681 cgggataatc cggaggacct gaagaaaatc ctagtaccgt caatgaacgg agcccaggtc
     1741 cctcttggca gtatagcaga aattgaatat acaaggggag cccagatgat acgaagtgag
     1801 aatactttcc ttgtcggtta tgtaatcttc gataagactg aaggcacagc agaagttgat
     1861 gtggtgaaac aggcagataa aatcctgaag gacaaaatta attcaggtga gatttcactt
     1921 cctgccggcg tgacttttaa atttgccggg aactatgagc accagctcag ggcaaccaaa
     1981 agactggcgc tggtgattcc tgtaagcctg atcttgatat tattgttgct ttacttccag
     2041 ttcaggacaa taactgcctc attcatacat ttttcggggg tctttgtcgc attctcaggt
     2101 ggattcataa tgctttggct ttatgggcag gactggttta tgaacttctc agttgcagga
     2161 ctgaaccttc gtgacatgtt ccagatgcat gaaataaacc tgagcgtagc ggtatgggta
     2221 ggtttcatcg cactgttcgg cattgccaca aatgacgggg taataatggg aacctatatt
     2281 caccaggtgt ttgaagacct taaaccgaag actgttcatg atgtgaggga ggctgtggtg
     2341 atggccggaa agaaaagggt ccggcctgca atgatgacaa ccgcggtggc tgttattgca
     2401 ctgttgcccg ttctttcatc aacaggaaag ggggcagaca taatggtccc gatggcaata
     2461 cccactttcg gaggcatgat aatacaggtt atgacaattt ttgttgttcc ggttttccag
     2521 gcaatctgga gggaagg
//
LOCUS       DAOPXU010000199         2473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_60756_length_2473_cov_12.866005, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000199 DAOPXU010000000
VERSION     DAOPXU010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60756_length_2473_cov_12.866005"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="PKK03_12355"
     CDS             <1..1035
                     /locus_tag="PKK03_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNX85231.1"
                     /translation="RVRASISAPQALKVQLDIGGVKYDLKKDDKGVWTGDSDPQDEGF
                     HYYQLVIDGAQVPDPGSMYFYGASRWGSGIEIPAYDQDFYALKKVPHGQVRENLYFSE
                     ITQSWRRCFVYTPPGYESSISTRYPVLYLQHGMGEDETGWSVQGRANLILDNLIADKR
                     AVPMIIVMDKGYASKPDQSSSRNAGGPQGRPGGISAFEEVLIKEIIPMIDTTYRTLSD
                     RSHRAMAGLSMGANQTIQITMNNLDLFSYIGGFSGTSNYPSSAEIDPATFMNGKFSDG
                     KALNEKINLFWLGLGTKEPDPFPGSVGAFRKMLEKTGVKYVYYESPGTAHEWLTWRRS
                     LYQFAPLLFR"
     gene            1083..>2473
                     /locus_tag="PKK03_12360"
     CDS             1083..>2473
                     /locus_tag="PKK03_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNX85232.1"
                     /translation="MKNLITILAFLAGFTLSSNSQDFEKQAPAGFDSVRAGIPHGKID
                     TIKYYSKVVGTTRKALIYTPPSYTRRSKYPVLYLLHGIGGDEKEWLNGGQPQVILDNL
                     YAEGKIEPMIVVMPNGRAMKDDSAGGNIMAPDKVEAFATFEKDLLYDLIPFIEKKYPV
                     LKDRDNRAIAGLSMGGGQALNFGLGNPDRFSWVGGFSSAPNTKPPEQLVPDPEKAKQA
                     LKVLWISCGDNDGLIIFSRRTHEYLTANDVPHIYYIEPGVHDFKVWKNGLYMFSQLLF
                     KPVDVSKFGKYSPLGTPAPSNVRGGKYPQIMPDGSALFRLKAPDAQKVQLDMAGKYDM
                     VRNGDGTWEVNTDSLTEGFHYYSLVIDGLTVADPASEAFYGMGRMVSGIEVPFSGDGY
                     YAAKSVPHGEIAIKRYFSTVFNTWRQFYIYKPAGYDRNTGQKYPVLYILHGGGEDERG
                     WATQGKTDLILDNL"
BASE COUNT          667 a          594 c          646 g          566 t
ORIGIN      
        1 cgggtccgtg caagcatatc agcaccacag gccctgaagg tacagctcga catcggaggt
       61 gtgaagtacg atctcaaaaa ggacgacaaa ggtgtctgga ccggtgattc agatccccag
      121 gatgagggat ttcactatta ccagctcgtc attgacgggg cacaggtccc cgatccggga
      181 agcatgtatt tctatggagc cagccgctgg ggcagcggga tagagatccc ggcctatgac
      241 caggatttct atgcgctgaa aaaagtaccg cacggccagg taagggagaa tctgtatttc
      301 tctgaaatta ctcaatcatg gagacggtgt tttgtctata ctccccccgg ttacgaaagt
      361 agtatctcaa cccgttatcc ggtgctctac ctgcagcacg gcatgggcga agatgagacc
      421 ggatggtcag tccagggcag ggccaacctg atccttgata acctgatagc cgataaaagg
      481 gctgttccga tgataattgt aatggacaaa ggttatgcat caaagccgga ccagtcatcc
      541 tcccgcaatg ccggagggcc acaggggagg cctggaggca tttcagcatt tgaagaggtg
      601 cttataaagg agataatccc gatgattgac accacgtacc ggactctgtc tgaccgcagc
      661 cacagggcta tggccggact ctcaatgggt gccaatcaga ctattcagat aacgatgaac
      721 aacctcgatc tgttttcata cataggcgga ttcagcggaa catccaatta tccgagttct
      781 gcggaaattg atcccgccac atttatgaac ggaaagttca gtgacggaaa agcattgaac
      841 gaaaagatca acctgttctg gcttggtctt gggaccaaag agccagatcc gttccccggt
      901 tcggtagggg ctttccgaaa gatgctggaa aagacaggag taaaatatgt ctattacgag
      961 tcacctggta cggctcatga atggcttaca tggagaagga gcctgtacca gttcgcgccc
     1021 ttgctcttca ggtaacagga gatactttgt ttttgccggt aaaaatgcaa atccatttgg
     1081 taatgaagaa tttaataaca atccttgctt tcctcgccgg gttcacccta tcatcaaact
     1141 cacaggattt tgagaagcag gctccagcag gatttgactc agtgagagcc ggaatccccc
     1201 acggtaaaat tgatacaatc aagtattact caaaggttgt gggcaccacg cgaaaggctc
     1261 tgatctacac tcctccctcc tacaccaggc ggagcaaata cccggttctc tacctgcttc
     1321 acggcattgg cggtgatgaa aaggaatggc tcaacggagg tcagccgcag gtaatcctcg
     1381 ataaccttta tgcagagggg aagatcgagc cgatgatcgt ggttatgcca aacggacggg
     1441 ccatgaagga tgacagtgca ggaggaaaca ttatggcgcc tgacaaggtg gaagcatttg
     1501 caacattcga aaaggatctt ctttatgacc tcataccctt cattgagaaa aaatatccgg
     1561 tgctcaaaga ccgtgataac cgtgcaattg caggtttatc aatgggcggc ggacaggcgc
     1621 tgaacttcgg tcttggcaat cctgacaggt tttcatgggt cggaggcttc tcatcagcac
     1681 caaatacaaa gcctccggaa cagcttgtac cagacccgga gaaggcaaaa caggcgttaa
     1741 aggttttgtg gatttcgtgc ggtgacaatg acggcctgat aatattcagc cgtcgaaccc
     1801 acgaatatct tacagccaat gatgttccgc atatatatta tattgaaccc ggggtgcacg
     1861 acttcaaggt ctggaagaac ggcctgtaca tgttttcaca gctcctgttc aaacctgtgg
     1921 atgtttcaaa attcggtaaa tacagtcctc tcggtactcc ggctccctcc aatgtcaggg
     1981 gagggaaata cccgcagatt atgcctgacg gcagtgccct gttccgcctg aaagctcctg
     2041 acgctcagaa ggtacagctg gatatggccg ggaaatatga catggtcaga aacggtgacg
     2101 gaacatggga ggtaaacaca gattccctga ccgaaggatt tcattattat tcccttgtca
     2161 ttgacggact gacagtagct gatccggcga gtgaggcatt ttatggcatg ggccggatgg
     2221 tcagcgggat tgaagtcccg ttcagcggtg acggctatta tgctgcaaag agcgttcctc
     2281 acggggaaat agcaattaaa agatattttt ccactgtctt caacacctgg cgccagttct
     2341 acatatacaa acctgccggc tatgaccgga ataccggtca gaaatatccg gtgctttaca
     2401 tccttcacgg cggaggggaa gatgagagag gctgggccac ccagggcaag actgacctca
     2461 ttcttgacaa cct
//
LOCUS       DAOPXU010000200         2440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_62070_length_2440_cov_13.246541, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000200 DAOPXU010000000
VERSION     DAOPXU010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62070_length_2440_cov_13.246541"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            317..1561
                     /locus_tag="PKK03_12365"
     CDS             317..1561
                     /locus_tag="PKK03_12365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNX85233.1"
                     /translation="MNANRKEATAARVESRLMGCSSDEEPGAGTMIFPQIGSHDRLVS
                     NRSSEFIRRYFPLANAESWNDWRWQLRNSFTSAEALSRVMTLTDDEINTLNRLKGRLP
                     LRITPYYLSLIYDSEPWSPLRRTMIPTSDEMIITDAEKADPLNEMGSSPVPGIVHRYP
                     DRALFTVTQFCSSYCRYCTRSHSVGKLGHLTKGEWELAFDYLREHTEIRDVIISGGDP
                     LTMNDSKIEYLLSNLRAIKHIEILRIGTKVPAVLPQRITPQLVNMLKKYHPLFLSVHF
                     THPDEMTPETRKACEMLADAGMPLGSQTVLLKGINDDPATMKSLMQKLLTVRVRPYYI
                     YQCDLIPGSSHFRTTIEKGIDIIENLRGHTSGYAVPQFVVDAPGGGGKIPILPAYVVE
                     SGPEKWVLRNYKKNRYTYPNKK"
     gene            1569..>2440
                     /locus_tag="PKK03_12370"
     CDS             1569..>2440
                     /locus_tag="PKK03_12370"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85234.1"
                     /translation="MRIGLTYDLRSWYLERGYTMDETAEFDKEETVVALENELVRLGY
                     ETVRIGNVFQLVEKLAAGERWDMVFNIAEGLYGDGRESVVPALLDQYRIPYVFSGPVV
                     MGVSLNKYLTRLVVAAAGVPVCPGIIANTLHDLNDLSSLEFPLFVKPVAEGTGKGITT
                     RSVVRDRESLVTLVSELLKAYNQPVLIEEYLPGREFTVGVTGNGAEAKVTGGMEVICK
                     DNLPYSVEVKENYENYVQYRVYDEDMRAECDAVALGAWRALGAVDAGRVDMKADRHGT
                     MCFIEVNPLAGMNPV"
BASE COUNT          643 a          625 c          624 g          548 t
ORIGIN      
        1 gaccggatga ttgaaaataa tctgaaaata atggctcaga aatatccata tatcagattt
       61 tgatttatgt ttgcgcctca aattccgggc atagatttca ccaccataat gaaggctgtg
      121 aataactcag actcagacag gttatgaaat tgaaaaccag atattatcag gcactgtttg
      181 catgttaata tcttttgacc ggagcagaaa ccgaaggtta tccaattaat aacttagcgg
      241 aaattttatg aaacatttta ataactaaaa cgaataatta acaaccagaa aaaaaggtaa
      301 agttttttac cgaagtatga acgctaacag aaaagaggca acggctgctc gtgttgagag
      361 ccgcctcatg ggttgttctt ctgacgaaga acctggcgcg ggtaccatga tcttcccgca
      421 gatcggatcc cacgatcgtt tagttagcaa ccggagttcg gaattcatac gccgctactt
      481 ccctcttgca aatgctgaat cctggaatga ctggcgctgg cagctgcgca actctttcac
      541 ttctgccgag gccctctcac gtgtcatgac actgaccgat gacgagatca ataccctcaa
      601 cagactgaag ggcaggttgc cgctaaggat cactccctac tacctgtcac tcatttatga
      661 ctctgaaccg tggtcaccgc ttcgccgaac catgataccg acgagtgatg agatgatcat
      721 taccgatgcg gaaaaggctg accctctcaa tgagatggga agcagccctg tgcccggtat
      781 agtccaccgt taccctgaca gggccctctt taccgtgaca cagttctgct cttcatactg
      841 caggtactgc acacgctcac attcagtggg caagctgggc catctgacca agggtgaatg
      901 ggaactggct tttgactatc tcagggagca taccgagata cgtgatgtca tcatcagcgg
      961 aggagaccct ctcaccatga atgacagcaa gatcgagtac ctgctttcaa acctgagggc
     1021 aatcaaacat attgagatcc tgagaatcgg aacgaaagtg ccggccgttt tgccgcagag
     1081 gatcaccccg cagctggtga acatgctgaa gaaataccat ccgctcttcc tcagtgtcca
     1141 cttcacccat cctgacgaga tgactcccga aacacgcaag gcatgcgaga tgcttgccga
     1201 tgcaggcatg ccgctgggaa gccagaccgt actgctcaag ggtatcaatg atgacccggc
     1261 aaccatgaag agcctcatgc agaagctcct caccgtgcgc gtaaggcctt actacatata
     1321 ccagtgtgac ctcatcccgg gctcaagcca cttcagaacc accatcgaaa agggtattga
     1381 catcatagaa aacctccgcg gacataccag cggatacgca gtgcctcagt tcgtggttga
     1441 cgcccccggc ggaggcggca agatacctat cctgccagct tacgtcgtcg agagcggccc
     1501 tgaaaaatgg gttctcagaa actataaaaa gaaccgctac acttacccca acaaaaagta
     1561 gttacgggat gaggattgga ctcacgtatg acctccgttc gtggtatctt gaacggggtt
     1621 ataccatgga cgagacggct gaatttgaca aggaggagac tgtggtcgcc cttgagaatg
     1681 agcttgtacg tcttggctac gaaaccgtac gcataggaaa tgtctttcag ctggttgaga
     1741 aacttgccgc cggcgaacgg tgggacatgg tattcaacat tgcagagggg ctctatggcg
     1801 acggccgcga gtcggttgta cctgccctgc ttgaccaata ccggattccc tacgtcttca
     1861 gcgggccggt ggtgatgggc gtctcactca acaaatacct caccaggctg gtggtggcag
     1921 cggcaggtgt gcctgtatgc cccggaataa ttgcaaacac cctgcatgat cttaacgacc
     1981 tctcttcact tgagttcccc ctcttcgtaa aacccgttgc tgagggtacg ggtaagggta
     2041 ttaccacccg aagcgtggta agagaccgtg aatccctggt aacgcttgtc agcgagctgc
     2101 tgaaagctta taaccagcct gttctgattg aagagtatct ccccggaagg gagttcaccg
     2161 tgggtgtcac cggcaatggc gccgaagcga aagttaccgg cggcatggag gtgatctgca
     2221 aggataacct tccttactcg gtggaggtca aggagaacta tgagaattat gttcagtaca
     2281 gggtatatga tgaggacatg agagcagagt gtgatgccgt tgcccttggc gcctggcgtg
     2341 ccctgggagc tgtagacgcc ggtcgcgtcg acatgaaggc agaccgccac ggaacgatgt
     2401 gcttcatcga agtcaatccc ctggcaggaa tgaaccccgt
//
LOCUS       DAOPXU010000201         2387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_64180_length_2387_cov_9.422384, whole genome shotgun sequence.
ACCESSION   DAOPXU010000201 DAOPXU010000000
VERSION     DAOPXU010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64180_length_2387_cov_9.422384"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1114
                     /gene="ftsY"
                     /locus_tag="PKK03_12375"
     CDS             149..1114
                     /gene="ftsY"
                     /locus_tag="PKK03_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNX85235.1"
                     /translation="MAFFGLFKKEEKEILDRGLSKTRENVFSRLTRAVAGKSKIDDEV
                     LDNLEEVLVSSDVGVETTLRIIDRVSKRVARDKYLTTTELNTILREEIAGLLEENKAT
                     HDVSSGFDAPLPSKPYVILVVGVNGVGKTTTIAKLAYNFRQAGRSVLLGAADTFRAAA
                     IEQLKIWSERAGVPMVSQQMGADPASVAYDTVKSAVAAGTDIVIIDTAGRLHNKVGLM
                     NELSKIRRVMEKVIPSAPHEVLLVLDGSTGQNAFEQARQFTAATEVNALAITKLDGTA
                     KGGVVIGISDQFRIPVKYIGVGEKMEDLRIFNRYAFVDSLFSDRA"
     gene            1114..>2387
                     /gene="rimO"
                     /locus_tag="PKK03_12380"
     CDS             1114..>2387
                     /gene="rimO"
                     /locus_tag="PKK03_12380"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005806287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNX85236.1"
                     /translation="MKHNRVNIVTLGCSKNTVDSQKLMRQLAAGGYNVMAESADPADI
                     VIINTCGFIHDAKEESVDTIIRHAEAIRREGRGRLFVMGCLVERYRNELQHELPEVDG
                     WFGVNRPQEVISQLGLAYRQELTGERIFSGPSHYAYLKVSEGCDRTCAFCAIPDIRGR
                     YISAPLEELVEEARLLASQGVKELILIAQDLTLYGTDLYGRQMLPELVRRLAAIDSLE
                     WIRLHYLYPADFPLEILEIMQENPKVCRYIDIPVQHISDRVLKMMRREHDSSGTRRLL
                     ADIRRMLPDAAVRTTVIAGHPGETEKDYKELLEFIREYRFERLGAFRYSHEENTHGAL
                     NYKDTIPERTKERRVAGIMGLQQEISAARNASMTGKVMKVIIDRREGLFHVGRSQHDS
                     PEVDQEILITTAGNIKPGIFIDVKITGSTEFDL"
BASE COUNT          618 a          570 c          700 g          499 t
ORIGIN      
        1 catatcagtt caaggaagaa gtagttcata acgaccacgt taaggacttt ttcacgaaga
       61 aataaggtca gtttttctga tcagttatac agggagacgc cggttagtgc gtctcttttt
      121 ttacatttgg ggcatcaaca gcagaagaat ggcatttttc ggattattta agaaggaaga
      181 gaaggagata cttgaccgtg gcctgagcaa aacacgtgaa aatgtctttt cgaggctcac
      241 cagggctgta gccggcaaat cgaaaattga cgatgaggtt ctggacaacc tggaggaggt
      301 acttgtctct tcggatgtag gggtggagac aaccctccgc ataattgaca gggtttcaaa
      361 gcgcgttgca cgcgacaagt acctgaccac cacggaactc aataccatcc tgcgggaaga
      421 gatagccggt cttctcgaag agaataaggc gacgcatgat gtctcctcgg gatttgatgc
      481 acctctgcct tcaaaaccat acgtgatcct cgttgttggc gtaaacggtg tgggcaagac
      541 cacaaccatc gccaaactgg catataattt caggcaagcg ggacgcagcg tgcttctagg
      601 ggcagccgac acgttccgcg ctgccgcgat agagcagctg aaaatatggt cagagcgtgc
      661 cggtgtgccg atggtgagcc agcaaatggg tgccgaccca gcttcagtgg cctatgatac
      721 cgtaaagtcg gcagtggctg ccggaactga tattgtgata atagatactg cgggacgtct
      781 tcacaacaag gtaggactga tgaacgagct atccaagata cggagggtga tggagaaggt
      841 gataccctcc gcacctcacg aagtgttact cgtccttgac ggatcaacag gtcagaatgc
      901 cttcgagcag gccaggcagt tcaccgcggc caccgaagtc aacgcactgg caatcaccaa
      961 gctggatggc actgcaaaag gcggggtggt gataggaatt tctgaccagt tcaggatacc
     1021 ggtcaaatac atcggggtag gcgagaagat ggaagacctg aggattttca accgctatgc
     1081 cttcgtcgat tcactcttca gcgaccgcgc ctgatgaaac ataacagggt caatattgtc
     1141 accctggggt gctcaaagaa taccgtcgat tcacagaaac tgatgcgcca gcttgcggcg
     1201 ggcggataca atgtcatggc tgaatcagcc gatcctgccg acattgttat catcaatacc
     1261 tgcggattca tccacgatgc gaaggaagag agtgtggata caatcatcag gcatgccgaa
     1321 gccatccgca gggagggccg gggcaggctg tttgtcatgg gatgcctcgt ggagaggtac
     1381 cggaatgagt tgcagcatga gctgcctgag gtggacgggt ggttcggggt gaacaggccg
     1441 caggaggtca tctcacagct tggcctcgca tacaggcagg aactgacggg tgaaagaatt
     1501 ttttccggac cctcgcatta tgcatacctg aaggtgtcag aagggtgtga ccgcacctgt
     1561 gccttctgtg caatacctga catacgcgga agatacatct cagccccgct ggaggagctg
     1621 gtggaggagg cacgcctgct cgcatcacag ggggttaagg aactgatact cattgcccag
     1681 gacctgactc tttacggcac cgacctgtac ggcaggcaga tgcttcccga gctcgtcagg
     1741 aggctggctg cgattgacag ccttgaatgg atcaggctgc actatctgta tcccgcagac
     1801 ttcccgctgg agatactgga gataatgcag gagaatccga aggtttgcag gtatattgat
     1861 atcccggtcc agcatatttc cgacagggtt ctgaagatga tgcgacgcga gcatgacagt
     1921 tcgggaacga ggaggctgct ggctgacatc cgccggatgc tgcctgatgc agcggtgcgc
     1981 accaccgtga ttgccggcca tcccggcgaa acagagaaag actataagga actgctggaa
     2041 ttcatccgtg agtaccggtt tgaaaggcta ggtgcgttcc ggtactcgca tgaggagaac
     2101 actcacggag cgctgaatta caaagacaca atccctgaaa ggacgaagga gaggagggtg
     2161 gccggcatca tggggctgca gcaggagata tcagctgcac ggaatgcctc catgaccggg
     2221 aaggtcatga aagtgatcat cgacaggaga gagggtctgt ttcatgtggg acgcagccag
     2281 catgattctc ccgaagtgga ccaggagata cttatcacca ccgccggaaa cattaaaccc
     2341 ggcatcttca ttgatgtgaa gataaccggg tcaacggaat tcgatct
//
LOCUS       DAOPXU010000202         2282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_68797_length_2282_cov_8.513247, whole genome shotgun sequence.
ACCESSION   DAOPXU010000202 DAOPXU010000000
VERSION     DAOPXU010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68797_length_2282_cov_8.513247"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2282
                     /locus_tag="PKK03_12385"
     CDS             <1..>2282
                     /locus_tag="PKK03_12385"
                     /inference="COORDINATES: protein motif:HMM:NF026036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNX85237.1"
                     /translation="WKNYRGGTVSTIWLFTQADRSVIRVPQPQGGCNDTHPMWVGDRI
                     YFLSDRNGEFNLFSCSGSGADIRQHTNFTDFPVLSASAATGRIVFEQAGYLHLYDIAR
                     GESQRIKTAIAADLLELRPRYVQGPGYIRSGHISPTGSRVVLGYRGEIITLPAEKGDP
                     RNITNTPGFHEKYPAWSPDGKTIAYFSDESGEYTLNLKSQDGKGEVKVYKLTGTGFYA
                     FPEWSPDSKRITFTDNGRNLWLLDVTTSVIKKIDSDEIYFPGPFREMHGDWSSDSRWV
                     VYTKMLNSFYRVVYLYSVDQQKSFPVTDGLSDASDPVFDPSGDYIYLFASTDAGPVIN
                     WFDLSSADMRMTNSIYLVTLRKDIISPFAKESDEEKGTEVKPDADKPAAVASDKSSGK
                     GSSKSAAKTSAAEKSDLLRIETDGLQERIVNVPVSAGNYSSLGVAGKGEILYIVSNPA
                     DQKSMLHKYNVSDRKDEEVMELDGYIVSADRKKMLYARGTTYGITAAGKKPEPGKGIL
                     NTGAISVRIDPKEEWPQIFGEAWRVNRDYFYDPGMHGADWKAMKDKYSQFLPHLTCRS
                     DLNRVIQWMCSELGVGHHRVGGGDRLNNPQRVGGGLLGADFSVENNRYRLRKIYGGLN
                     WNPDLRSPLTEPGLNVKTGEYILAVDGRDVTADENIHRFLENTDGKIVELTVGPNPDY
                     TGSRVIKVVPIGNEISLRNRDWVEGNLRKVTEATDGKVAYVYVPNTSGQGHEYFKRYF
                     FPQANRKAVIIDERFNGGGM"
BASE COUNT          610 a          556 c          639 g          477 t
ORIGIN      
        1 gtggaaaaac taccgcggag gaaccgtttc gaccatctgg ctcttcaccc aggccgacag
       61 gtcagtgatc agggtgccgc agccgcaggg aggatgcaat gacacccatc ccatgtgggt
      121 cggtgacagg atatattttc tcagtgaccg taacggagag ttcaacctct tttcatgcag
      181 cggcagtggc gctgacatca ggcagcacac caatttcaca gacttccccg tgcttagcgc
      241 ttcagctgca acgggcagga tagtctttga acaggccgga taccttcatt tatatgacat
      301 cgccaggggc gaatcacaaa ggataaagac agctatagca gctgacctgc ttgaactgcg
      361 cccgcgttat gtccagggtc cggggtacat ccggagcggg cacatctctc cgacgggaag
      421 ccgcgttgtg ttggggtaca ggggagaaat catcaccctt cctgccgaaa agggagatcc
      481 gaggaatatc accaacacac ccggttttca tgaaaaatac ccggcatggt cacctgacgg
      541 aaaaacaatt gcctatttct cggatgaatc cggtgaatac accctgaatc tgaaatcaca
      601 ggacggcaag ggcgaggtta aggtatacaa actgacaggg acgggattct atgccttccc
      661 ggagtggtcc cctgacagca aaagaattac cttcacagat aacggaagga acctgtggct
      721 tcttgacgta acaacctcag taataaagaa gatcgattct gacgagatat actttccggg
      781 accgttcagg gagatgcacg gcgactggtc atcagactcc aggtgggtcg tgtacacgaa
      841 gatgctgaac agcttttaca gggttgtgta cctctattct gttgaccagc agaaatcatt
      901 tcccgtcact gacgggctga gcgatgcctc tgatcccgtc tttgacccga gcggagacta
      961 tatctattta ttcgcatcca ctgacgccgg tccggtcatt aactggttcg acctttcaag
     1021 cgcagacatg aggatgacaa actcaattta tcttgtcacc ctcaggaagg acattatttc
     1081 gccctttgca aaggagagtg atgaagaaaa gggtacggaa gtcaaacccg atgctgataa
     1141 acctgcagcc gttgcgtctg acaagagctc cggcaagggt tcatcaaaga gcgcagcaaa
     1201 gacatccgcc gcagaaaaga gtgatctgct caggatagaa acagacggac tgcaggaaag
     1261 aattgtaaac gtaccggtat ctgccggcaa ttacagttca cttggggtag cgggaaaagg
     1321 tgaaattctt tatatcgttt ccaaccctgc agaccaaaag tcgatgcttc ataaatacaa
     1381 tgtctccgac aggaaagatg aggaggtgat ggagctcgac ggctatattg tgtcagctga
     1441 caggaagaag atgctgtatg ccaggggtac gacctacggg atcaccgccg cagggaaaaa
     1501 accggagccg ggcaagggca tactgaatac cggggccatc tcggtcagga tagatccgaa
     1561 agaagagtgg cctcagatat ttggtgaggc atggcgggtc aaccgcgact atttctatga
     1621 tcctggcatg cacggggccg actggaaggc aatgaaggac aagtattctc agttccttcc
     1681 tcacctgacc tgcaggagtg atcttaacag ggtcatccag tggatgtgca gcgagcttgg
     1741 cgttggccac cacagggttg gcggtggtga caggctgaac aacccgcaga gggtcggagg
     1801 cggcctcctc ggcgccgact tctcggtgga gaacaacagg taccggctga ggaaaatcta
     1861 cggcggactc aactggaacc ccgatctcag atcacctctt accgaaccgg gattgaatgt
     1921 taagaccggg gaatatatac ttgctgttga cggccgcgat gtcaccgcag atgaaaacat
     1981 ccacaggttt ctcgagaaca cagacggtaa aatcgttgag cttaccgttg ggccgaatcc
     2041 ggattacacg ggttcccgtg tgataaaggt tgtacccatc ggtaacgaga tcagcctgag
     2101 gaaccgtgac tgggttgagg gtaacctcag gaaggtaacg gaggcaaccg acggtaaagt
     2161 ggcttatgtg tatgtgccaa acacatccgg gcagggtcat gagtacttca agaggtactt
     2221 tttcccgcag gccaacagga aggctgtcat cattgacgaa cggttcaatg gcggggggat
     2281 ga
//
LOCUS       DAOPXU010000203         2247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_70533_length_2247_cov_10.699818, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000203 DAOPXU010000000
VERSION     DAOPXU010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70533_length_2247_cov_10.699818"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1617
                     /locus_tag="PKK03_12390"
     CDS             <1..1617
                     /locus_tag="PKK03_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85238.1"
                     /translation="LLLLFLPLNGQKKSELKEIWAEAESHYLYGEYELANPLYLILNE
                     LRPDNFNIKYKIGNCYLNIFDEKPKAIPFLEEAVRSTSYNSKTGKLKEMRAPLDAYFS
                     LANAYRITNRLDSAMNTYRFFSQLITEASSMQNEEFINQQIQACNVASDQMKNPVRVR
                     IQPLKEYINQGSVNDRPVVSFDGSTMAYTERRGLESVVYVTRREGTSWGTPDDITLEA
                     SMGTDCHTTSLNNDGTELYLFKNDNYDGNIYVTRYTDGKWSPIEKLNRNINTRFYESH
                     AAISSDGKKLYFTSNREGGLGELDLWVSEKDITGDWGLPSNLGNVVNTPYNEETPFIS
                     GDGNTLTFSSEGHGSMGGYDIFLAKLSNNQWTTPVNIGYPVSTTDDNIAFQPFDNGRL
                     GFYSIMTGYKKKEIALVDFNVPDEEERDTVTISFNPADLPYLTVVDSSMLITDLIVRD
                     VKDTDAEIDPDVLYYTVQVMALYNPVDPAYFEYAEISVFYNREDRFYRYTTGRFDTKP
                     SAYEEKDRLLRLGYPDDIFVKKVYRNDSGK"
     gene            1601..2131
                     /locus_tag="PKK03_12395"
     CDS             1601..2131
                     /locus_tag="PKK03_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX85239.1"
                     /translation="MILENDGIRLRAIEPEDLELLYRWENDETNWKQGNTLVPYSRYI
                     LKKYIAGSGKSIFETGQLRLMIDVLPDERTIGTLDLYDFDQYHNRAGVGILIADESDR
                     QKGYASAALACMIKYAFETIGLHQLWCNILESNAPSLNLFTHHGFSICGSRKEWAKVS
                     DGYETEHLLQLINPHK"
BASE COUNT          651 a          572 c          539 g          485 t
ORIGIN      
        1 ttgctgctcc tgtttttgcc gcttaacggg cagaagaaaa gtgagttgaa ggagatatgg
       61 gcggaagccg aatctcacta cctgtatggt gagtatgaac tggccaaccc gctttatctc
      121 attcttaatg aactcagacc tgataatttc aatatcaagt acaagatcgg caactgctac
      181 ctcaatatct tcgatgagaa gccgaaagcc attccgttcc ttgaagaagc tgtcagaagc
      241 accagttaca actccaaaac cggcaagctt aaggagatgc gtgctcccct tgatgcctac
      301 ttctcgctgg caaatgccta ccgcatcacc aacaggcttg acagcgccat gaacacctac
      361 aggttcttca gccagctgat aactgaagca tcatcaatgc agaacgagga gttcatcaac
      421 cagcagatac aggcctgcaa cgtggcctct gaccagatga aaaatcctgt cagagtacga
      481 atccagccac tgaaggaata tatcaaccag ggctcagtga acgacaggcc ggtggtcagc
      541 tttgacggca gcacaatggc atacaccgaa agaagaggtc ttgaaagcgt ggtctatgtc
      601 acccgcaggg agggaacatc atggggaaca ccggatgaca taacccttga ggcatccatg
      661 ggaaccgact gccacaccac ttcactgaac aatgacggca ccgaactcta cctgttcaag
      721 aatgacaact acgacggcaa tatctatgtg accaggtata ccgacggcaa atggtcgccg
      781 attgaaaaac ttaacaggaa tatcaatacc agattctacg aatcgcacgc tgccatatca
      841 tcagacggga agaaactcta cttcacctcc aaccgcgagg gcggcctggg tgaacttgac
      901 ctgtgggtct cggaaaagga catcacggga gactggggac tcccttccaa cctgggaaac
      961 gtagtgaata ctccctacaa cgaagagacg cccttcattt caggagatgg caacacactg
     1021 acattcagct cagaagggca cggatcgatg ggtggctatg acatcttcct cgcaaaactg
     1081 tcaaacaacc agtggaccac cccagtcaac ataggatacc ctgtcagcac gaccgatgac
     1141 aacatcgcct ttcagccgtt cgataacggc aggctgggct tctactcaat catgaccgga
     1201 tacaagaaga aggagatagc tctcgttgat ttcaatgttc cggatgagga ggaaagagac
     1261 actgtcacca tcagcttcaa tcctgcagat ctgccctatc tgacggttgt tgactcatca
     1321 atgctcatca ctgacctgat tgtcagggat gtaaaggata ctgatgcgga gattgacccc
     1381 gatgtgctct attacacggt gcaggtaatg gcgctgtata atccggttga tccggcctat
     1441 tttgagtatg cggagatctc ggtgttctat aacagggaag accgattcta ccgctacaca
     1501 accggcaggt ttgatacaaa gccaagtgca tacgaggaaa aggaccggct gctccgcctc
     1561 ggatatccgg atgatatctt tgtgaagaaa gtctacagga atgattctgg aaaatgacgg
     1621 gatcagactt cgcgcaattg agcccgaaga cctggaactg ttgtaccgct gggaaaacga
     1681 cgaaaccaac tggaaacagg gaaacaccct tgtaccctat tcgaggtata tcctgaaaaa
     1741 gtacattgcc ggatcaggaa aaagcatttt tgagaccggt cagctcaggc tcatgataga
     1801 cgttctgccc gatgaacgga caataggcac tcttgaccta tacgactttg atcagtatca
     1861 caatcgtgcc ggtgtcggta tcctcattgc tgatgagtct gacaggcaga agggatacgc
     1921 ttcggctgcc ctggcctgca tgataaaata tgcctttgaa accatcggtc tgcaccagct
     1981 ctggtgcaac atcctggaga gcaacgctcc cagcctgaat cttttcaccc atcacggctt
     2041 ctctatctgc ggaagcagga aagagtgggc caaagtgagc gacggttacg aaaccgaaca
     2101 ccttctccag cttatcaatc cacataaata aatactgccc agggggatgg gcagtattat
     2161 tgtctcttcc ggcaaaaaga agtaccattg tcagccggag agaggaaacg gggaatctaa
     2221 aaaacactaa tccttaaact cctctgt
//
LOCUS       DAOPXU010000204         2224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_71620_length_2224_cov_10.825265, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000204 DAOPXU010000000
VERSION     DAOPXU010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71620_length_2224_cov_10.825265"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..77)
                     /locus_tag="PKK03_12400"
     tRNA            complement(5..77)
                     /locus_tag="PKK03_12400"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:Gly,seq:gcc)
     gene            complement(176..>2224)
                     /gene="mutS"
                     /locus_tag="PKK03_12405"
     CDS             complement(176..>2224)
                     /gene="mutS"
                     /locus_tag="PKK03_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNX85240.1"
                     /translation="ERFGSRFYTYRLDDWIFTPDTANDRLLKQFGTTSLKGFGVQGMT
                     SGIIAAGAILWYLDMTRHENLGHINRLARIEEEKYVWLDRFTVRNLELFHSPYEGAKT
                     LIEILDRTLSPMGGRMMKRWLSLPLKDISVLNTRLDIVEFLIGHPDVKEEMEGLIREV
                     GDLERLLSKVAVGRVTPREVAQIRRALAAIGQLKTLCAASDFQPLVSMASGFDPCPEL
                     ADTIGRTLTPDPPVAVSKGHVIAEGISQELDDLRKIYYGGKDYLNDLQQREGIRTGIN
                     SLKISYNNVFGYYIEVRNAHKDKVPPDWIRKQTLVSAERYITEELKEYEEKILGAGEK
                     ILALETSLFNDLVVSIGPYINLMQADAATVGRLDCLRSFAVVSDEYGYIRPVLDDSHV
                     LDIKDGRHPVIERQLPPDDPYVPNDVWLDNDEQQVIILTGPNMSGKSALLRQTALIVI
                     MAQTGCFVPAKEARIGIIDKIFTRVGASDNLSLGESTFMVEMNEAASILNNLSDRSLV
                     LLDEIGRGTSTYDGISIAWAMVEYIHECRHRAKTLFATHYHELNEMESTFSRVRNFNV
                     SIRELDNRVIFLRKLKRGGSEHSFGIHVARMAGMPKSVVGRADEILKMLEEGRGGTNL
                     GKPVEEIATHREGYQLSFYQLDDPVLKQIRDELMGLDLDNLTPMEALNKLYNIRKLLR
                     "
BASE COUNT          487 a          684 c          517 g          536 t
ORIGIN      
        1 tctgagcggg aaacgagact cgaactcgcg gccccgacct tggcaaggtc gtgctctacc
       61 aactgagcta ttcccgcaat tgtgactgca aaaataatac ttttttcaaa aatacaaaca
      121 acgaaatttt aagcgacagg atcacattcc ggctactgat caaatggata aaaccttatc
      181 tcaataattt ccgtatgttg tacaatttgt tcaacgcctc catcggtgta aggttgtcaa
      241 gatcaagccc catcaattca tcacggatct gtttgagcac cgggtcatcc agctggtaga
      301 agctgagctg atacccctcc cggtgcgtgg caatctcctc aaccggcttg cccagattcg
      361 tgccgccacg cccctcctca agcatcttca gtatctcatc cgccctgcct acaacactct
      421 tcggcatgcc ggccatccgt gctacgtgta tcccgaaact gtgctcactc cctcccctct
      481 tcagcttgcg gaggaaaatc accctgttgt cgagctcccg tattgagaca ttgaaattcc
      541 tgaccctgga aaaggtgctc tccatctcgt taagctcatg gtaatgagtg gcaaagagcg
      601 tcttggccct gtgcctgcat tcgtggatat attccaccat ggcccaggca atggagatgc
      661 cgtcataggt gctcgttccc ctccctatct catcaagcag cacgagactc ctatccgaca
      721 gattattgag gatgctggca gcctcattca tctcaaccat aaaggtagac tcaccgagag
      781 acaggttatc cgatgcaccc acccgtgtaa atatcttgtc aattattcca atcctcgcct
      841 cctttgccgg cacgaaacag cctgtctgtg ccatgatcac tatcagggct gtctgcctga
      901 gcaacgccga cttacccgac atattcggac cggtcagtat gatcacctgc tgctcatcat
      961 tgtcaagcca tacgtcattg ggcacatacg gatcatccgg cggcagctgg cgctcaatca
     1021 ccgggtgacg cccgtccttg atatcaagta catggctgtc gtccagcacc ggccttatgt
     1081 agccatactc atcagaaacc accgcaaatg accgcaggca gtcaaggcgc cccacagttg
     1141 cagcatcagc ctgcatcagg tttatgtagg gccctattga aaccaccagg tcattgaaca
     1201 gcgaggtctc cagcgccagt atcttctccc ccgccccaag gattttctcc tcatactctt
     1261 ttagctcctc cgtgatatat ctttcggcgc tcaccagggt ttgcttgcgt atccagtccg
     1321 gtggcacctt gtccttatgt gcattgcgca cctcgatata ataaccgaat acattgttat
     1381 aactgatctt cagggagttg attccggtcc gaataccctc tctctgctgc aggtcgttca
     1441 ggtaatcctt ccctccgtag tagattttcc ggaggtcatc cagctcctgt gaaatgcctt
     1501 cggcaatcac gtgtcccttg ctgactgcaa ccggcggatc gggagtcagt gtccggccga
     1561 tggtgtctgc cagttcgggg cagggatcga atcccgaggc cattgagacc agcggctgga
     1621 aatccgaggc tgcacacagc gtcttcagtt ggccgatagc cgccagcgca cgccgtatct
     1681 gcgccacctc acggggagtg accctcccca ccgccacctt gctcagcagc ctctcgagat
     1741 cacccacctc gcgtatcagc ccctccatct cctctttcac atcgggatgg ccaatcagga
     1801 actccacgat atcaagcctg gtattcagta ccgaaatgtc cttcagcggc agtgagagcc
     1861 accgcttcat cattcttccg cccatgggcg aaagcgtccg gtcaaggatc tctatcagtg
     1921 tctttgcgcc ctcgtagggc gaatggaaca gttcgagatt gcggacagtg aacctgtcaa
     1981 gccatacgta tttctcctcc tcgatacgcg cgaggcggtt gatgtggcca aggttctcgt
     2041 gcctggtcat atcaaggtac cagaggattg cgcctgcggc gatgatgccg ctggtcatcc
     2101 cctgcacacc gaagcccttc agcgacgtgg tcccgaactg cttcagcagc ctgtcgttgg
     2161 ccgtatcggg ggtaaatatc cagtcgtcaa gcctgtatgt gtaaaaccgg ctgccgaacc
     2221 gctc
//
LOCUS       DAOPXU010000205         2170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_74347_length_2170_cov_12.135697, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000205 DAOPXU010000000
VERSION     DAOPXU010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74347_length_2170_cov_12.135697"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1028
                     /locus_tag="PKK03_12410"
     CDS             72..1028
                     /locus_tag="PKK03_12410"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNX85241.1"
                     /translation="MLRRKNYVFDHANLQFLQHRRNGREKLLRFALWLSLSIVATLFY
                     SLIFHRLFGSPKEELLQQQVEELRLQYTMLEKQIDHSTAAVAELRSTDNASYRAVLDL
                     PVIPADISEGGSGGTNRTADLTGYMNSDLMMKVRSKFDDLKTQTNIQYNSLTELTARA
                     SEWKEMWEHLPYIRPVNVTLRLGDGMKFREKHPVLGSPRWHFGQDFCCPIGTEVHATG
                     AGVVAYAGNQRDGFGIKVVIDHGYGYRTIYGHLSEFNVRQGQKVSRGDFIGLSGNTGT
                     STGPHLHYQIDLFGEHVNPLWYFQDNLTEDEYFMMLEYLSKP"
     gene            1135..2106
                     /locus_tag="PKK03_12415"
     CDS             1135..2106
                     /locus_tag="PKK03_12415"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNX85242.1"
                     /translation="MPRTKYKFNHETLSFDRIRLGLRQTFLRLFGYFVASLFLAGIYG
                     FIFAFIFDSPQEKALKREIAQLTLQYELMNREMENVEKVLTHLQETDDNLYRTIFETE
                     PVPSSYREGGIGGVNRYQELEGFSNSELVVETARRLDRIRKKIYVQSESFDELVAYAR
                     EKEEMLSSIPAIQPLSNKDLKRTASGFGYRIHPIYKISKFHSGMDFTAPTGTDVYATG
                     NGVIRTVKSARRELGNHVVIDHGFGYQTVYAHLDRFNVKEGQKVKRGDVIGFVGSTGL
                     STAPHLHYEVLANGQYVDPALYYFNDLTPEEYERMLEISGKSGQTFD"
BASE COUNT          569 a          504 c          568 g          529 t
ORIGIN      
        1 aaaattaaat caaatatttt acgtttgcag aatagtcatc ttaaaatggt ggggggtcca
       61 cggatctgct gatgcttaga aggaaaaatt acgtttttga tcatgcgaac ctgcagttcc
      121 tgcaacacag gagaaacggg agggagaagc ttctccggtt cgcactgtgg ttgtctcttt
      181 cgatagtagc cactcttttt tacagcctca tctttcaccg gttgttcggt tcgcccaagg
      241 aggagttgct tcagcagcag gtggaagagc tgaggttaca gtacaccatg cttgaaaaac
      301 aaattgatca ctccacagca gcagtggctg aacttcgttc cactgacaat gcctcataca
      361 gggcggtact cgacctgcct gtcataccgg ctgatatctc tgaaggagga tcgggaggta
      421 ccaacaggac ggccgatctg accggttaca tgaactctga tcttatgatg aaggtaaggt
      481 cgaagtttga tgacctgaaa acgcagacaa atattcagta caactcactt acagaactta
      541 cggcaagggc atccgagtgg aaggagatgt gggagcatct gccgtatatc aggcccgtaa
      601 atgtcaccct gcggcttggt gacggcatga agttccgtga aaagcatccg gtgcttggtt
      661 caccacgctg gcatttcgga caggatttct gctgtccgat cggcaccgag gtgcatgcaa
      721 ccggggctgg tgttgtggcc tatgcgggca accagcgtga cggattcggg ataaaggtgg
      781 ttattgacca cggatatggc tacaggacta tctacgggca cctgagcgaa ttcaacgtca
      841 ggcagggtca gaaagtctcc cggggtgact tcataggatt atccggcaac acaggaacat
      901 cgaccggacc ccacctgcat taccagatag acctttttgg cgagcatgtg aatcccctct
      961 ggtacttcca ggacaacctc acggaggatg aatatttcat gatgctcgaa tacctcagca
     1021 aaccctgacg ggaaaatttt aatcaatttt taacgttatc agcagtcata actgtatttg
     1081 tattttgcta ttatttctta tgtttgcagg gtatagagca tgccatccat aaaaatgccc
     1141 aggacaaaat acaagtttaa tcatgagaca ctcagctttg acaggatcag gctgggcctc
     1201 aggcagacat tccttaggct tttcggatat tttgtcgcat ccctgttcct cgccggaatt
     1261 tacggtttca tctttgcctt catctttgac tccccgcagg aaaaggccct gaagagggag
     1321 atagcacagc tcacccttca gtatgagctg atgaacaggg aaatggaaaa cgtggaaaag
     1381 gttctgacac accttcagga gacagatgac aatctctacc ggactatttt tgagaccgaa
     1441 ccggttccgt catcataccg tgaaggaggg atcggagggg ttaaccggta ccaggaactc
     1501 gaaggctttt cgaattccga actggttgtg gagactgcca ggcggcttga caggatcagg
     1561 aaaaagatat atgtccagtc cgagtcgttt gacgaactgg ttgcctatgc cagggagaag
     1621 gaggagatgc tgagcagtat ccctgcgata cagcctctct cgaacaagga tctgaagcgt
     1681 acggcatcag gttttggcta tcgcattcac ccaatataca agatatcgaa gttccattcg
     1741 ggaatggact ttacagcgcc cacgggaaca gatgtttatg ccacgggcaa cggggtcatc
     1801 cggacggtca agtcggcccg ccgcgaactc ggtaaccatg tggtcattga tcatgggttc
     1861 ggataccaga ccgtttatgc tcatcttgac cggttcaatg tgaaggaagg gcagaaagtc
     1921 aagagaggag atgtgatagg atttgtgggc agcaccggcc tttccactgc tccgcacctg
     1981 cactatgagg ttcttgccaa cgggcagtat gttgacccgg ctctctacta cttcaatgac
     2041 ctcacgcctg aggaatatga acggatgctc gagatttctg gcaagagcgg gcagaccttc
     2101 gactgattca tttaactgat catgccttac agggaaaaga aagtggaaaa gctcttttac
     2161 cagatcggtg
//
LOCUS       DAOPXU010000206         2162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_74758_length_2162_cov_17.269103, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000206 DAOPXU010000000
VERSION     DAOPXU010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74758_length_2162_cov_17.269103"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2027)
                     /locus_tag="PKK03_12420"
     CDS             complement(<1..2027)
                     /locus_tag="PKK03_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX85243.1"
                     /translation="MSHPRVSGAIGPGLKAEVPGIEAACRLNSIGTNVFYAEDLNSYE
                     ADVSLSDEFLFDVLPRQVVMGNPAEILKTPLACMVSEKIAKAIGGDVIGKVITLKRYP
                     DKKLTIAGVFKELPENTNYHYDLLISMVSTKHFTWDGTDNWMGNDRYYTCVKLEPGVA
                     PESLAPAVRKMQEVHQDIVRLETEYGGSILKYSFLPIRRLYSGGVTDMIIILSTIAIA
                     VLFVSLMNYILLTLSALVTRAKSSAIHKTFGAQARTLQLIVFVETLILFFISMAGAVL
                     IIAALKPVIESQLGHSIAAVMNPYVILPLLAIVTLLVLITGWFPGRFFSRIPVATVFR
                     SYKQKNNRWKLALLSVQFAGASFILTLMIFVTLQFGMMKNADHGYNVDGVYYGSTVGM
                     DGNRLQTILNELRALPEVENVGLGYGLPLNGASGNNVRPHDGQMELFNVADFYEADEN
                     FLSILDIKVSAGQNFSPGNTAVNDIVISEKGSDMLKMHFGWTDGVVGKQIHITEHGST
                     TVCGVFPDFIINSIAEPDTRPAVFFYLPEERFVQMKIEKPASSFLILVKVREGLESGM
                     KKEIADVMNLGMPQKDATVRSLEEDLENMYNAQKGFRNALMAGNAVILVILVIGLLGY
                     TTSEAGRHQKELAVRKISGATTSDILKVFILELEYIAIPSVLAGLIGVWFTV"
BASE COUNT          569 a          571 c          458 g          564 t
ORIGIN      
        1 acggtaaacc aaaccccgat caggcctgca agaacagatg ggatggcaat gtactccagc
       61 tcaagtataa aaactttaag gatatcagaa gtagtggcgc cgcttatctt tctgacggcc
      121 agttccttct gatgcctgcc ggcctcactt gttgtataac caagcagacc gataaccagg
      181 atgaccagaa taaccgcatt ccccgccatc aatgcattcc ggaacccctt ctgggcatta
      241 tacatgttct caagatcctc ctcaaggctc ctgacggtgg catctttctg aggcattccc
      301 aggttcatta catccgcaat ctcctttttc ataccagatt ccaacccctc acgcaccttg
      361 acaagtatca ggaatgaaga agccggtttc tcgattttca tttgtacgaa cctttcttct
      421 ggcaggtaga agaaaaccgc aggccgcgtg tcgggttccg ctattgaatt gattatgaaa
      481 tcggggaaaa cgccacagac agttgttgat ccgtgttcgg ttatatggat ttgttttccc
      541 acgaccccgt ccgtccagcc aaagtgcatc ttcagcatat ctgacccctt ctcactgatc
      601 acaatatcat ttacagccgt attgccgggt gaaaagtttt gtccggctga taccttaata
      661 tccaaaatcg aaaggaaatt ctcatctgcc tcgtaaaaat cagcaacatt gaacaactcc
      721 atctgtccgt catgtggtct tacattatta cccgaagcgc cgttaagtgg gagaccgtaa
      781 cccaggccta cattttccac ctcgggcaac gcccgaagtt cgttcagaat ggtctgaagc
      841 ctgttaccat ccatcccaac cgtagatccg taatagactc catccacatt gtacccatgg
      901 tctgcgttct tcatcatgcc gaattgcagt gttacgaaga tcatcagggt aaggatgaac
      961 gaggcgcctg caaactgaac ggagagcaat gccagtttcc acctgttgtt tttctgcttg
     1021 tatgaccgga atacggttgc caccggaatt cgcgaaaaaa acctgcccgg gaaccatccg
     1081 gttatcagta caagtaatgt aacaattgcc agcagcggta atataacata agggttcatc
     1141 acggcagcga tactgtgccc gagctgtgat tcaataaccg gcttcaatgc tgctatgatc
     1201 aggacagctc cggccattga aatgaagaaa agtatcaggg tttcgacgaa gacaatcagt
     1261 tgcaacgttc tggcctgtgc cccgaatgtc ttatggatgg cagatgactt tgccctggtt
     1321 acaagtgcac tgagggtcag cagaatataa ttcatcagcg aaacgaaaag cacagcgatt
     1381 gcaattgtgc tgagaatgat tatcatatcc gtcacacctc cggaatataa tctccttatt
     1441 ggcaaaaacg aatacttaag gatactccct ccgtactccg tctccagcct gacaatatcc
     1501 tgatgtacct cctgcatttt ccgtactgca ggcgcaaggc tctcaggagc gacaccgggc
     1561 tcaagtttta cacatgtata gtacctgtca ttacccatcc agttatctgt tccgtcccag
     1621 gtaaaatgct tcgttgatac catcgagatg agtaaatcat aatgatagtt tgtattctcc
     1681 ggcagctctt tgaatactcc tgcaattgtc agtttcttgt cgggatacct cttcagggtg
     1741 atcaccttcc cgattacgtc tccacctatc gccttagcaa ttttctccga taccatacat
     1801 gccagtggcg ttttcagaat ctcagccgga ttccccatga cgacctgccg gggcagaaca
     1861 tcaaagagaa attcgtcgct cagggacacg tctgcttcgt aactgttaag gtcttcagca
     1921 taaaatacat ttgtgcctat cgaattcagt cggcaggccg cttcaatacc ggggacctca
     1981 gccttcagac cgggtccgat ggcgccgctg acccttggat gactcatcag tttttccgat
     2041 gacttatccg gtttgaaatt ctcatgaaca acataaattc gtccggcatc cggatagaag
     2101 ctgtcgtaac tgtaataata caatacttcg gcaatgagca atatgccaaa agccagtccg
     2161 gc
//
LOCUS       DAOPXU010000207         2138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_76041_length_2138_cov_13.055209, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000207 DAOPXU010000000
VERSION     DAOPXU010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76041_length_2138_cov_13.055209"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PKK03_12425"
     CDS             complement(<1..290)
                     /locus_tag="PKK03_12425"
                     /inference="COORDINATES: protein motif:HMM:NF012310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNX85244.1"
                     /translation="MAQNYNFPELPYAYNALEPYIDAQTMEIHYTKHHRAYFDNFVKA
                     AGEAKIDNLTLEDIFSRISIYSPAIRNNGGGYYNHMLFWKVMGPNAGGQPEG"
     gene            478..1317
                     /locus_tag="PKK03_12430"
     CDS             478..1317
                     /locus_tag="PKK03_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNX85245.1"
                     /translation="MKWQGRRGSDNVEDRRGMSTGTKVVGGGIGTLVIVLLVYLLGGD
                     PSGILQQSGSTETAGPVEASEEENAMAQFVSVVLADTEEVWGKIFAQSGATYRQPTLV
                     LFRDQVQSACGYATAASGPFYCPGDEKVYIDLSFFDDLRAKYGVYGDFAIAYVVAHEI
                     GHHVQNQMGILDEVQNERQRLSEVRGNQLTVRLELQADFLAGVWAHYASKMFNSIEEG
                     DIEEALNAAASIGDDVLQKKYQGRVVPDSFTHGTAAQRSSWLRKGWETGDLNQGDTFS
                     GAI"
     gene            1486..>2138
                     /locus_tag="PKK03_12435"
     CDS             1486..>2138
                     /locus_tag="PKK03_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85246.1"
                     /translation="MKRFLILLVLVSAAAVVRGEEPLSSEGLQKKYPANVNKNQRPKS
                     LAASWPRWAVHSNLLGFVQFGPVVTAEYNITRQLTLSAHARFSSVGALTPILHQAEAD
                     NGGRPDNFSGIAFGGGPLWFFKFRKDKAYVGLIGEYEMSDVLYLEDYVNEWNQDNRKI
                     IAMVNAGYRFMFGQKLIPASSRKGDLFLRDGLFLNAGVYFGAERNQYRWAYTDPEADG
                     "
BASE COUNT          506 a          489 c          590 g          553 t
ORIGIN      
        1 tcgccttcag gctgaccgcc ggcgttggga cccatcacct tccagaagag catgtgattg
       61 tagtaaccgc ctccgttgtt gcgtatggca ggtgagtaga tgctgattct tgagaaaata
      121 tcctcaagag tcaggttatc aattttagcc tcgccggctg ccttgacgaa gttgtcaaaa
      181 taagccctgt gatgcttcgt gtagtgaatc tccatcgtct gggcgtcaat gtatggctcc
      241 agcgcattgt atgcatacgg gagctccgga aaattataat tctgtgccat aattttgttg
      301 tttacggtta ttaatcctag tacaattgtg aaagtcagaa tagccttcgt tttcattttc
      361 ttgttgttta tttgttaaac agatgatcag cgaaattgtt caatgctaaa tttttcaaat
      421 attgaatcga tttagtattt tcgtcaccat taagaataac tcataataca taagactatg
      481 aaatggcagg ggcgtcgcgg cagcgacaat gttgaagacc gcaggggtat gagtaccgga
      541 acgaaggttg tcggaggcgg gataggcacg ctagtaattg tgttgctcgt ttacctcctc
      601 gggggagatc cgtcgggcat tctgcaacaa tcgggctcca cagaaacggc tggtccggtg
      661 gaggcctctg aagaagagaa cgcaatggct cagtttgtct cggtagtgct ggctgacact
      721 gaagaggtct ggggcaagat attcgcccag tctggagcca cataccggca gcccaccctg
      781 gtgctgttca gggatcaggt ccagtcggcc tgtggctatg ccaccgccgc cagcggaccg
      841 ttctactgcc cgggtgatga aaaggtatac atcgacctca gtttctttga tgatctgcgt
      901 gcaaaatatg gtgtttacgg tgacttcgcc atagcttatg tcgtggcgca cgagataggg
      961 catcatgtcc agaaccagat ggggatcctt gatgaagtgc agaacgagcg tcagcgcctg
     1021 agcgaggtca gaggcaacca gcttactgtc aggcttgagc tgcaggctga ttttctggca
     1081 ggtgtatggg cccactatgc aagcaagatg ttcaactcca ttgaagaggg tgacatagag
     1141 gaagcactta atgccgcggc ttccatcggc gacgacgtgc ttcagaaaaa ataccagggc
     1201 agggtggtac ctgattcctt cactcacggc acggcagcac agcgctcgag ctggctccgc
     1261 aagggatggg agacgggtga cctgaaccag ggcgatacct tctcaggcgc aatctagaca
     1321 ccggcgacag gtacttttgc agcttccggg gataatgcgt gccactcact gcaccggccg
     1381 acctgcattg cattaccggt cagatgaggg ttgattatca catgcagatg taatatcatt
     1441 acctgcctga cgttttctct tttagacagt taaacaaatt tagtgatgaa gaggtttttg
     1501 atcctgctgg tccttgtctc tgctgctgcg gttgtcaggg gtgaggagcc gttgtcttct
     1561 gaaggattgc agaaaaagta ccctgccaat gtcaacaaga atcaaagacc caaatcactt
     1621 gctgccagct ggcccagatg ggcagtgcac tcaaacctcc tcgggtttgt gcagttcgga
     1681 cccgttgtga ctgctgaata taacattacc aggcaattaa ccctcagtgc acatgccagg
     1741 ttttcatccg tcggtgcgct gactcctatc ctgcatcagg ccgaggccga taatggtggc
     1801 aggcctgaca atttttcggg tattgcgttt ggcggcggac cgctatggtt tttcaagttc
     1861 cggaaagata aggcatatgt cggtctcatc ggcgaatacg agatgtcaga tgtgctttac
     1921 cttgaagatt acgtgaatga atggaaccag gataaccgca agatcattgc gatggttaat
     1981 gccggttaca ggttcatgtt tggtcagaag cttattcccg catcgtcgcg aaaaggtgat
     2041 cttttcctga gggatgggct gttcctgaat gccggggttt atttcggagc tgagaggaac
     2101 cagtatcggt gggcatatac tgacccggag gcagatgg
//
LOCUS       DAOPXU010000208         2129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_76552_length_2129_cov_13.405979, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000208 DAOPXU010000000
VERSION     DAOPXU010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76552_length_2129_cov_13.405979"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PKK03_12440"
     CDS             <1..390
                     /locus_tag="PKK03_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85247.1"
                     /translation="EKDLSNLRVIGYRNVWFRFLPSEANVFVPVSLNSMAVLNQSFRD
                     CYVSQVNASFPSPAYDGPFSHLSQQVWVEQFKQVQLILGKDFFYENESPKIRGTHGMV
                     YYKEMPVDEFLQHARELEKSVEGANIH"
     gene            complement(462..1718)
                     /locus_tag="PKK03_12445"
     CDS             complement(462..1718)
                     /locus_tag="PKK03_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNX85248.1"
                     /translation="MKTSGRAIISASLALLITFLTSCHATGQVVETGIEVMASNNFEI
                     LQGKRVGLVTNPTGVDRNLRSTVDIINSAPGVKLTALYGPEHGVRGEFTAGEYVESET
                     DPGTGLPVYSLYGKTRKPSPDMLNNIDILVYDIQDIGSRSYTFISTLGLLMEAAAEKG
                     IPVVVLDRPNPLGGIRMEGAVTRPGFFSFVSQFAIPYLHGMTVGELAMFLNGEGMLAN
                     GTRCDLHVVKMNGWSRKMMFEETGLQWIPSSPHVPHRDTPLYYPATGIAGELYAVSIG
                     VGYTIPFQTFAAEWINADSLAAALSALKLPGVIFRPVHYKPYYSTLQGKLVHGVQIHL
                     TEPSEAPLSLIQFYIMQEAHRLWPDENLFEICDPSRHQMFDKVCGTDEVRKAFTRAFR
                     VDDILNLWNSDIPAFRARASKYFLYK"
     gene            1921..>2129
                     /locus_tag="PKK03_12450"
     CDS             1921..>2129
                     /locus_tag="PKK03_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85249.1"
                     /translation="MKPLTYLVLFFVLFQFAGCQKKEVPAELTEKLEAIAAEMVPQHA
                     ESLCDVSLVMSEGGILTAKGETNLP"
BASE COUNT          526 a          509 c          542 g          552 t
ORIGIN      
        1 gagaaggatc tttcgaatct gagggtgata ggttaccgta acgtctggtt cagatttctg
       61 ccgtctgaag ccaatgtctt cgtgcccgta tccctcaact caatggcggt gctgaaccag
      121 tcgttccgtg actgctatgt cagccaggtg aacgcatcat tcccgagtcc cgcatatgac
      181 ggaccgttca gtcacctgtc ccagcaggtg tgggtggaac agttcaagca ggtgcagctg
      241 atactgggca aggatttctt ctatgagaat gagagtccca agatcagggg aacgcatggt
      301 atggtgtatt acaaggagat gcctgtcgat gagtttctgc agcacgcccg tgagcttgaa
      361 aagagcgtcg aaggggcaaa catccactga cagggtggtc aggtcagtgg caaaatacag
      421 gaatgcagag ctatagacct gaccgatgca ggccgggaaa ttcacttata cagaaaatac
      481 ttcgaggccc tggccctgaa tgccggtatg tcactgttcc acagattcag gatgtcatcc
      541 accctgaatg ccctggtgaa tgcttttctg acctcgtcag tgccgcacac cttgtcgaac
      601 atctggtgcc ttgacggatc acatatttca aagagattct cgtcaggcca caggcgatgt
      661 gcctcctgca tgatataaaa ctgtatcagt gacagtggag cttctgacgg ttccgtcagg
      721 tgaatctgca ctccgtgaac cagctttccc tggagtgtgc tgtaataggg tttgtaatga
      781 accggtctga agatcactcc ggggagcttc agtgcgctga gtgcggcagc gagtgagtct
      841 gcattgatcc attctgccgc aaaggtctga aagggtattg tgtatcccac cccgatactg
      901 acggcataga gttctccagc tatgccggtt gccggatagt acagcggggt gtcacggtgc
      961 gggacatgag gagaggaggg aatccactgc aggcctgtct cttcgaacat catcttcctt
     1021 gaccatccgt tcatcttcac cacatgaaga tcgcacctgg ttccattggc aagcatccct
     1081 tcgccgttga ggaacatggc gagctctccc acagtcatac cgtgcagata cggaatggca
     1141 aactggctga caaatgaaaa gaatccgggg cgggtgactg ctccttccat ccttatcccg
     1201 ccaagaggat tgggacggtc tagtaccacg acaggaatcc ctttttcggc agctgcctcc
     1261 atgagcaggc cgagagtact tataaaggtg tatgaccttg acccgatatc ctggatgtca
     1321 tagaccagga tatctatgtt attcaacatg tctggtgaag gttttcgcgt ttttccgtag
     1381 aggctgtaaa caggaagacc ggtgccggga tcagtctcgg attcaacata ttctccggct
     1441 gtgaactcac cgcgtacacc gtgctcgggc ccgtaaaggg ccgtcagttt aacccctggt
     1501 gcagagttta ttatgtcaac cgttgacctg aggttgcggt caaccccagt gggattggtc
     1561 accagaccca cccttttccc ctgaaggatt tcgaaattgt tactggccat tacctctatt
     1621 cctgtctcca caacctgccc ggttgcatgg caggaggtaa gaaatgtaat taaaagtgcc
     1681 agtgacgcag agatgattgc tcttcctgag gtcttcataa gctgttgatt ttatgttgta
     1741 taccggttgc ctggccggca taataaggcc gaaacaagca gttttcaatt ctgataatac
     1801 aagtcccggc actgccggta ataaaatttc tgtctgacaa attaaataaa attcaattgc
     1861 ctattgactt gaatattata aatatttaat tttggatttg taagatgtaa tacaactttc
     1921 atgaaaccct taacctatct cgtcttattt ttcgttcttt ttcagtttgc aggttgtcag
     1981 aaaaaagagg ttccggcaga attgacggaa aagcttgagg caatagcagc tgaaatggta
     2041 ccccagcatg ccgagtcact ctgtgatgtc agtcttgtta tgtctgaagg aggcattctt
     2101 acggctaaag gagaaacaaa tctcccgga
//
LOCUS       DAOPXU010000209         2058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_80631_length_2058_cov_13.923615, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000209 DAOPXU010000000
VERSION     DAOPXU010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80631_length_2058_cov_13.923615"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..>2058
                     /locus_tag="PKK03_12455"
     CDS             284..>2058
                     /locus_tag="PKK03_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85250.1"
                     /translation="MKNCLLHVVAVLFLLPVFASAQQPEMKNGVRPDGVVTVQDLHPS
                     EQAVHPAAARAMARGEKPGTFRNVSYELAPNVRMLTPAQKSRIRLQQDGILDLSGGAF
                     RPSPDSVYLDPESDLVMQAEPVDDIRMLALRPSMSAVFQDIDVPEQEVPLTLANTVSM
                     AEGVIESSLQLNNKYAVNLRFDSVTFVLDKTDKDSLLATLVGQILITNPRVEGKYTKN
                     NGYRLVFLASEQVDMKIYTSMKLKEEAKTPIWGTVIEMSDLGKCEIGLFILATLEGHV
                     TLAVEIHQGIEMAMGAKGNTFWYLPTSIKNVSTIDSWCDIGYDITSQMKAFIGFQCTA
                     NLKVKGYNALDVYVNGGMEGTVATDGMTLDADVGLRLKAGGKIVSKKFTLLDSYYSLW
                     KYKKPDFHGYSMVIHEACAWGDYVAGEVHTLVPGDVPGVTEKVPYKGSLTLLVSRPDG
                     SVDQITSVTGDDGVFIARNVPLKNGDKVRIRLPDVPNPGPELSVTIPFREVKLYAADY
                     FTGVAEGNVAGSGSKWAKLAGAQQGSSPAAASAFAGGSPADRFKGAVPQQEAVRRLNE
                     FRNNLTIYHGPIEFITSSGPEVRLAG"
BASE COUNT          496 a          484 c          548 g          530 t
ORIGIN      
        1 gtatttgccc tgggcataca tcttgtgctg aagctcacct atctcctcca gcatcttttc
       61 agtcttcttt ttaatctcct tcttgtccca ttccttgtta aatgtggggt gctctgatat
      121 tttcatgatt ttacgctttt gattcatcat ctgctaaatt aggaattaaa gctgaggaat
      181 tcagatttcg aacaaaaatg cacagttttt taaaattcag cattgctgat atattaaacc
      241 tgtgattatc tgcgttgtaa aaaagcacta acccctaaac atcatgaaaa attgtctttt
      301 acacgttgtt gctgtccttt ttctgttgcc tgtctttgct tcagcacagc agcctgagat
      361 gaaaaacggt gtcaggcctg acggtgtggt taccgttcag gacctgcatc catcggagca
      421 ggctgtacat cctgctgcag cgcgtgccat ggcgcgcggt gagaaacccg gtaccttcag
      481 gaacgtcagc tatgaactgg ctccaaatgt caggatgctt actcctgccc agaagagccg
      541 tatcaggctg cagcaggacg gcatactcga tctctccggc ggagctttca gacccagccc
      601 ggattctgtt tatcttgatc ctgagagtga ccttgtaatg caggcggagc cagtggatga
      661 catacgcatg ctggcactcc ggccatcaat gtcagccgtt tttcaggaca ttgacgtacc
      721 tgaacaggag gttccgctga cacttgcaaa caccgtgtca atggctgagg gtgtcattga
      781 aagttccttg cagctgaaca acaagtatgc ggtcaatctc cggtttgaca gtgttacttt
      841 cgtcctggac aagactgaca aggactcact tctggcgaca ctcgtcggac agatactgat
      901 cacaaatccc cgtgttgagg ggaagtatac gaagaacaac ggttaccggc tcgtcttcct
      961 ggcttcagag caggttgaca tgaagatcta taccagcatg aagctgaagg aagaggccaa
     1021 aaccccgata tggggaacgg tgatagagat gtcagatctt ggtaagtgcg agataggttt
     1081 gtttatactt gccaccctgg aaggtcatgt gaccctggcg gtggagatcc accaggggat
     1141 tgagatggct atgggagcca agggtaatac tttctggtac ctgcccacga gcatcaaaaa
     1201 cgtttcaact attgattcat ggtgtgacat tggttatgat atcacctcgc agatgaaggc
     1261 ctttataggc ttccagtgca ctgctaacct gaaggttaag ggttacaatg cccttgatgt
     1321 ctatgtcaac ggagggatgg aggggactgt tgccactgac gggatgaccc ttgatgccga
     1381 tgtggggctc aggctcaagg ctggcgggaa gatagtatca aagaaattta cgctgctcga
     1441 cagctactat tctctctgga aatacaagaa accagatttt cacggttaca gcatggttat
     1501 tcatgaagct tgtgcctggg gagactatgt tgccggggag gttcatactc ttgtgcccgg
     1561 agatgtaccc ggtgtgacgg aaaaagtacc gtacaagggc agcctcactt tgctggtcag
     1621 ccggccggat ggttcggtgg atcagatcac atccgtcacg ggtgatgacg gagtctttat
     1681 tgcacgtaat gtgcctctga agaatggtga caaggtgagg atcaggctgc ctgacgtgcc
     1741 aaatcccggt cctgagctca gtgtcacgat acccttcagg gaggtaaaac tctatgcagc
     1801 ggattacttc acaggtgtgg ctgaggggaa cgttgccggt tcaggcagca aatgggcgaa
     1861 acttgccgga gcacagcagg gatcgtcgcc tgccgcagca tccgcttttg caggcggatc
     1921 tcctgctgac cgcttcaaag gggctgttcc gcagcaggaa gccgtcaggc gtctgaatga
     1981 gttcagaaac aatcttacca tttaccacgg tccgatagag ttcataacca gttccggacc
     2041 tgaggtgagg ctggccgg
//
LOCUS       DAOPXU010000210         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_81109_length_2050_cov_14.401003, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000210 DAOPXU010000000
VERSION     DAOPXU010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81109_length_2050_cov_14.401003"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..581)
                     /locus_tag="PKK03_12460"
     CDS             complement(90..581)
                     /locus_tag="PKK03_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HNX85251.1"
                     /translation="MSQLRGPAELYQLLDSLQIPYEYIEHPPVPTVEEAMKYWSGIDS
                     GHCKNIFFRNHKGDRHYLVILYYDSKLNIRELEQKLRQGKLSFASDRRLMQYLGLTPG
                     SVSPFGLINDRSRHVHLFIDKTLINHERLSFHPNTNTASLIIPREGLFRFLEHTGNTW
                     ELF"
     gene            complement(578..982)
                     /locus_tag="PKK03_12465"
     CDS             complement(578..982)
                     /locus_tag="PKK03_12465"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HNX85252.1"
                     /translation="MKSFLHKATDLTPEIILSPAENRFIIAGKSAPEDVRGLYYPVIE
                     WMQEFVAEIKRGHNYTDANPLRLKLDLEYFNSSSAKFLFDIFGHLKEMSNEGIPVDIE
                     WHYDEEDTDLREAGEDLALLAELQFRYCPKTR"
     gene            complement(1045..1527)
                     /gene="rnhA"
                     /locus_tag="PKK03_12470"
     CDS             complement(1045..1527)
                     /gene="rnhA"
                     /locus_tag="PKK03_12470"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNX85253.1"
                     /translation="MSPSVVSIYTDGACSGNPGPGGYGVVLLSGSLRKELSGGFRLTT
                     NNRMELLAAITGLEALKLDGLVVRLYTDSQYLVNAVNNGWLFSWEKKRFAKKKNPDLW
                     IRFLEVYRRHRVTLVWVKGHNNDPENERCDTLAVAASKLPGLPEDEGYKANGDDEEII
                     "
     gene            complement(1524..2048)
                     /locus_tag="PKK03_12475"
     CDS             complement(1524..2048)
                     /locus_tag="PKK03_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNX85254.1"
                     /translation="MDMPGSIPIILDIKSKTGNIHPWKATLVSLSIFLAFLIFGEKLL
                     SIFGIDVGSFAIAGAFIIFLIGMEMVLGAEFFKHDSPGGGTIVPIAFPLIAGAGSITT
                     ILSLRAEYATINILIALMLNMVIIYTVLRLTTRIERLLGPTGLHILKKFFGIILIAIA
                     IRLFINNTGITLPQ"
BASE COUNT          512 a          501 c          520 g          517 t
ORIGIN      
        1 attcttttca tggcagtaag ggttaaaaat caagatgcaa gtccgtcata aagactgccg
       61 gacagtgcat agtgctgcat gccatgtgat cagaataatt cccaggtgtt gccagtatgc
      121 tccaggaacc tgaaaagccc ctcacgggga ataatcagtg atgcggtgtt tgtgttcggg
      181 tggaatgaca gccgctcgtg gtttatgagc gtcttatcta tgaagagatg gacgtggcgt
      241 gagcggtcat tgataagtcc gaacggagag accgaaccgg gcgtcagtcc gagatactgc
      301 attaacctcc tgtcggaggc aaacgagagc ttcccctggc gaagcttctg ctcaagctcc
      361 ctgatgttca gctttgaatc ataatagaga atcaccaggt aatgcctgtc acccttatga
      421 ttgcggaaaa agatgttctt gcagtggccg gagtcgatgc cggaccagta tttcattgct
      481 tcctcaaccg tcggcacggg aggatgctct atatattcat agggtatctg cagcgaatca
      541 aggagctggt aaagttctgc gggtcctcta agctggctca tctggttttc gggcaatacc
      601 tgaactgcag ttcagcaagc agcgccaggt cctcgccggc ctcgcgaaga tccgtgtctt
      661 cctcgtcata atgccattcg atgtccacgg gaatcccttc gttgctcatc tccttcaggt
      721 ggccgaagat gtcaaagaga aactttgctg aggatgagtt aaagtattca agatcaagct
      781 taaggcggag cgggttggcg tcagtgtaat tgtgtcccct ttttatttcc gcaacgaact
      841 cttgcatcca ttcaataacc gggtaataaa gacccctgac atcttcaggc gccgacttcc
      901 cggcgataat gaaccggttt tctgcaggag acaggattat ctccggcgtc aggtctgttg
      961 ctttgtgtaa gaaacttttc atctctcccc tttttttaca aaaattgcac tttttttata
     1021 aaagacaaat attactgtca cctgtcagat gatctcttca tcatcaccat ttgccttgta
     1081 gccttcatct tcaggaagac ccggaagctt tgatgctgcc accgcaaggg tatcacatcg
     1141 ttcattttca gggtcattgt tatgaccttt cacccacaca agggtcaccc tgtgacgcct
     1201 gtaaacctcc aggaaacgga tccacagatc agggtttttc tttttggcaa accgtttctt
     1261 ttcccagctg aagagccagc cgttgttgac cgcattgaca aggtattgtg aatcggtgta
     1321 caaccttaca accagcccgt caagtttgag cgcctcaagt ccggtgatgg ctgccagaag
     1381 ctccatccgg ttgttggtgg tgagcctgaa acctcctgag agctccttgc ggagcgaacc
     1441 cgaaagaagc accacaccgt agcctcccgg accgggattg ccgctgcagg caccgtcagt
     1501 atagatagaa accactgacg gactcattgc ggaagggtga tgcctgtgtt gttgatgaac
     1561 agccttatgg cgatggcaat aaggataata ccgaaaaatt tcttcagtat gtgcagcccg
     1621 gtgggcccca gcaaacgctc gatcctcgtc gtgagcctga ggacggtata tatgatcacc
     1681 atgttcagca tcagggcaat aaggatgttt atagttgcat actcagccct gagagaaagg
     1741 atggtggtaa tggatccggc accggcaatt agcggaaagg ctatcggcac aatggttccg
     1801 cccccgggcg aatcatgctt gaagaactct gcaccgagca ccatttccat cccgatcagg
     1861 aaaatgatga atgcacctgc tattgcgaac gaacctacgt caataccgaa tatgctgaga
     1921 agtttctccc cgaagataag gaatgcaagg aaaatggaaa gcgagaccag ggttgccttc
     1981 caggggtgga tgttgccggt cttagatttg atatccagga tgatagggat cgatcccggc
     2041 atgtcaatga
//
LOCUS       DAOPXU010000211         2000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_84223_length_2000_cov_11.296658, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000211 DAOPXU010000000
VERSION     DAOPXU010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84223_length_2000_cov_11.296658"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PKK03_12480"
     CDS             <1..825
                     /locus_tag="PKK03_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HNX85255.1"
                     /translation="LEGDITSITAVELIDQDPIGKSSRSNPVTYLKAFDDIRKLFAEQ
                     QASVQAGFKPAHFSFNTEGGRCEECQGEGIIRVEMQFMADVELTCEACKGMRYKRDIL
                     DVRFNGKNIYDVLGMTIEEAIEFFGKSKDKTCHRIAAKLRPLADVGLGYVRLGQSSST
                     LSGGESQRVKLAFYLSQEKSTEKMLFIFDEPTTGLHFHDISKLMKSLNALVEHGHSVI
                     LIEHNVEVIKCSDWVIDLGPEGGEKGGYVVFEGTPEQLAQCDESYTAKFLKQKLNC"
     gene            complement(842..>2000)
                     /locus_tag="PKK03_12485"
     CDS             complement(842..>2000)
                     /locus_tag="PKK03_12485"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNX85256.1"
                     /translation="NMSLTDKFTLEQAVITNVLPERILSSHMISISGENFNPDPQMNI
                     VEIGGSTAIVTNCTATQMTVSIPSELEEGFYRVSVTTIAGSPVTWDKEIEVVKSVWSR
                     LADFPASGRVAAAGFAAGGRIYFGTGLEPNLKDKNDFWEFDPATSAWTRKMDFPVLIT
                     YATAFSIEETGYFTMGKQGSVYYHMLYRYDPDDDLWTQMRSNPVNSSAMDSPGFVING
                     KAYVPAGGEMYEYDPASDQWTKKSYPEALGYFGGGSAFTINGKGYLGVGWAQEKSADV
                     SDFYEYDPGTDSWTRKASFPGTLRNNATSFSLPNGKGYIALGYSEEQSKYLNDVWEYD
                     PVLDTWTRTEDFPGSPRFGARAVVVGSGAYIIGGYGGVYEKDMWLFSPRGK"
BASE COUNT          522 a          505 c          485 g          488 t
ORIGIN      
        1 ctcgagggag acatcaccag catcaccgcc gtggagctga tagaccagga ccccatcggc
       61 aaatcaagcc ggtcaaaccc cgtaacctac ctgaaggcct tcgatgacat ccgcaagctt
      121 tttgccgagc agcaggcctc tgttcaggca ggattcaaac cggctcactt ctccttcaac
      181 actgagggag gccggtgcga ggagtgccag ggcgaaggaa tcatcagagt ggagatgcag
      241 ttcatggccg atgttgagct tacatgcgag gcatgcaagg ggatgcgcta taaacgcgat
      301 atcctcgacg tcaggttcaa cgggaaaaat atttacgatg ttctcggaat gaccatcgag
      361 gaggctattg agttcttcgg caaaagcaag gacaaaacct gccacaggat tgccgcgaaa
      421 ctaagaccgc ttgctgacgt gggacttgga tacgtcaggc tgggacagtc atcatcaacc
      481 ctctcgggcg gtgagagcca gagggttaaa ctggcattct acctcagcca ggaaaagagc
      541 accgaaaaga tgcttttcat ctttgatgag cccaccacag gcctccactt ccatgacatc
      601 agcaagctga tgaagtcgct caatgcactt gttgagcatg gacattcggt gattctcatc
      661 gaacataacg tagaggtgat caaatgttct gattgggtaa ttgaccttgg tcccgaggga
      721 ggagagaagg gcgggtatgt tgtgtttgag gggacacctg aacagttggc gcagtgcgat
      781 gaatcgtaca cagcaaaatt tctgaagcaa aagctcaatt gctgaccgga gatatcagga
      841 attattttcc ccgtggtgaa aacaaccaca tgtccttctc atagactcct ccgtaaccac
      901 cgatgatata agcgccagag ccaactacga cagccctcgc tccgaatcgg ggtgaaccgg
      961 gaaagtcctc ggttcttgtc catgtgtcaa gcaccgggtc atattcccat acatcattca
     1021 ggtattttga ctgctcttct gaataaccca aggcaatgta tcccttaccg ttaggcaggg
     1081 agaaggatgt ggcattattc cttagtgtgc cggggaaact ggctttcctg gtccacgagt
     1141 ctgttcccgg atcatactca tagaaatcac ttacatcggc agatttttcc tgagcccatc
     1201 caaccccaag gtagcccttg ccatttattg tgaacgccga accaccacca aaatatccga
     1261 gagcttcagg gtacgacttt tttgtccact ggtcagatgc aggatcatac tcatacatct
     1321 ctcctcctgc cgggacatat gcctttccgt tgattacaaa tcccggagaa tccatagccg
     1381 aggaattaac agggtttgaa cgcatttgtg tccataaatc atcatcagga tcatatctgt
     1441 aaagcatatg gtagtagaca gaaccctgtt tgcccatggt aaaatatcct gtttcttcaa
     1501 ttgagaaggc tgtggcatag gtaatcagta ccgggaaatc catcttcctt gtccaggcag
     1561 acgtcgcagg atcaaactcc cagaagtcat ttttatcctt aagatttggc tccaaaccag
     1621 ttccgaagta gatcctgcct cctgctgcaa aacctgcagc tgcaacccga ccgcttgccg
     1681 gaaaatccgc cagcctcgac cagactgact tgacaacctc aatttctttg tcccaggtta
     1741 ccggcgatcc ggcaatagtg gttactgata cccggtaaaa tccttcttca agttctgaag
     1801 gtatggagac tgtcatttgt gtggcggtac aattggttac gatcgcagta ctgcccccga
     1861 tctcgacaat gttcatctga gggtcgggat taaagttctc accagaaatg gatatcatat
     1921 gcgagcttag tattctttca ggaaggacat tggttatcac tgcctgctca agggtgaact
     1981 tgtcggtcag tgacatgttt
//
LOCUS       DAOPXU010000212         1992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_84727_length_1992_cov_12.380485, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000212 DAOPXU010000000
VERSION     DAOPXU010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84727_length_1992_cov_12.380485"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="PKK03_12490"
     CDS             complement(<1..1421)
                     /locus_tag="PKK03_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX85257.1"
                     /translation="MLLHQQFVRIAKKNASKLAVIDKTTGKSVTYSRALIGALILSSK
                     FSKYDKGFIGIMIPTSAGAALATVGALMSGRVPVMINYSTGAEANVRYARKKCRFTTV
                     ITSRSLLEKINCPLVEGMVMIEDLMAGVTTGDKLKAALKTKLPLGMLLNTVSRGSEDD
                     TAAMLFTSGSEKDPKAVPLSHRNLIANIESFSSYVRVSGEDKILANLVFYHIFGLTVN
                     LWVAFYHGMTMVTYANPLDFQKISTIAREEQPTIMVGTPSFFWGYLNKSEPGDFKSLR
                     LMVAGADKCPDALRDGYMKKHGVTLLEGYGATETSPVISVNSQEFNRAGSTGKVIPGI
                     QVRIESIESGEECKVREIGKILVKGPSVMRGYYDDPAQTAEVLKDGWYNTGDMGYLDE
                     DGYLWHAGRFKRFTKVGGEMVSLVKVENVMEKYLHQGVSCCVVEVPDDKKGSYIVAAV
                     SAEVNKTEILRRMMADLPSIALP"
     gene            complement(1720..>1992)
                     /locus_tag="PKK03_12495"
     CDS             complement(1720..>1992)
                     /locus_tag="PKK03_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85258.1"
                     /translation="ATKNKIRVLGLEINKTAYNTIMWILVGGLILLMAIGFLIFKRNL
                     VVLLRTEKDLKELKDEFAAYRQSSRIAREKVEMDLFRANQKLKGLV"
BASE COUNT          444 a          544 c          445 g          559 t
ORIGIN      
        1 ctcggcaggg caatgctcgg caggtcagcc atcatcctcc tgagtatctc ggtcttgttt
       61 acctctgcag atactgccgc cacaatataa gaacccttct tatcgtcagg cacctcaacc
      121 acgcaacaag acaccccctg atggaggtat ttttccatca cattctccac tttcaccagc
      181 gaaaccattt ctccacccac ctttgtgaac ctcttgaacc ggcctgcatg ccagagatag
      241 ccgtcttcat ccaggtagcc catatcgcct gtgttgtacc agccgtcttt caggacctca
      301 gccgtctgtg cgggatcatc atagtagcct ctcatgacgg aaggtccctt gacaagtatc
      361 ttgccgatct ccctgacttt gcactcttct cctgattcaa tactctctat ccttacctgt
      421 atgcccggga tcaccttccc cgtgctgccc gctctgttga actcctgtga attgaccgag
      481 atcaccgggg aggtttccgt ggcgccgtat ccctcgagaa gagtgacacc gtgcttcttc
      541 atatagccgt cgcgcagcgc atctggacac ttgtctgcac ctgctaccat cagcctgagt
      601 gatttgaaat cgcccggctc cgatttgttc aggtaacccc agaagaaact cggggtacca
      661 accatgatgg ttggctgctc ctcgcgtgct attgtgctta tcttctgaaa atcgagcgga
      721 ttggcgtagg tcaccatcgt catgccgtga taaaaagcca cccacaggtt gacggtcagg
      781 ccaaatatgt gatagaatac gaggttggca agtatcttgt cctccccgct gactctcaca
      841 tagctgctga agctttctat gttggcaatg aggttacggt gagacagggg cactgccttc
      901 ggatccttct cgctgccact ggtaaaaagc attgcagctg tatcatcctc gctgcccctg
      961 ctgactgtat tcagcagcat tcccagcggc agcttcgtct tcagtgcagc cttgagcttg
     1021 tcgccggtgg tgacgcctgc catcagatcc tctatcatga ccataccctc caccagcggg
     1081 cagttgatct tctcaagcag ggaacgcgag gtgataacgg tggtaaacct acacttcttc
     1141 cgggcatacc tgacgttagc ctccgctccg gttgagtagt ttatcatcac gggaacgcgt
     1201 ccgctcatca gcgctcccac tgttgcaagt gcagcgcccg cagatgttgg aatcattatc
     1261 ccgataaatc ccttatcgta tttgctgaat ttcgagctca gtatcagagc ccctatcaac
     1321 gcccttgaat atgtgacgct ctttccggtt gtcttgtcta ttactgccag cttcgaagca
     1381 ttcttcttcg ctatcctgac gaattgctga tgtagtaaca tgttgattcg gttttaattc
     1441 ttgtctttcc tctgtgtcag gggctgaaga taaagaatta aaccaataca aaatgcattg
     1501 atgaatattt cattcgaaat ttattgtaaa tatcacatga tcttatacca ttgtttatgt
     1561 gttctttaca aagctcttaa ggaaacatat tcaatgccat atatatttcg ttcgaatttt
     1621 gttccgaaat gcgaaaatgg ctttgtatga tttataagaa ggggcggagg gctgaaaagg
     1681 gcagctatgc cccggcagaa taccggcaaa agggtgctgt cagaccagcc ccttgagctt
     1741 ctggtttgcg cggaagaggt ccatctccac tttctcgcgg gcgatccttg acgattgcct
     1801 gtaggcagca aattcatcct tgagctcctt caggtctttc tctgtgcgga ggagtacaac
     1861 caggtttctt ttaaagatca ggaatccaat tgccatgagc agtatcaatc cgcccaccag
     1921 tatccacata atggtgttat aggctgtttt atttatctcc aggccaagta cccggatctt
     1981 gtttttcgtg gc
//
LOCUS       DAOPXU010000213         1971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_86094_length_1971_cov_12.148747, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000213 DAOPXU010000000
VERSION     DAOPXU010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86094_length_1971_cov_12.148747"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PKK03_12500"
     CDS             complement(<1..773)
                     /locus_tag="PKK03_12500"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX85259.1"
                     /translation="MMRIVICGPAHPYRGGIAAYNEKLAQQLLQEGDAAAIYTFKLQY
                     PSFLFPGKTQYDHGYAPEGVPITRAINSVNPLNWFNIGLRLRRTRPDLVIMRYWLPFL
                     APALGFIARLVRSNGHTRVITIFDNVVPHEKRFGDTMLTRFFVKSIDGALVMTRAVED
                     DLRQFAADMPCVISPHPLFDNYSVPVSRAEAIVRLKLCPADKFILFFGFIREYKGLDL
                     LLRAMAEQCVRDLGVKLIVAGEFYESEKPYFDLIHQLETG"
     gene            complement(770..>1971)
                     /locus_tag="PKK03_12505"
     CDS             complement(770..>1971)
                     /locus_tag="PKK03_12505"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX85260.1"
                     /translation="TKIPPDRRVPLTPSQVVELKDKYPDVEFYVQPSDIRCFTDDEYK
                     YLKIPIREDLSNCDILMGVKEVDKRAFIPGKTYLFFAHVAKKQEHNLEMLREILSRKV
                     KVIDYEYLTTDKGQRVVAFGRWAGIVGAYNALRARGIKTNRFKLKPAYQCHDLNEMWA
                     GLRLIQLKPGLKILVTGEGRVANGAMETLANCNNLLKVSIEDFLSKEYDVPVVCQVGP
                     QHYVKHHDGRPFDFNNFVQHPEDYRSDFLKYTKVTDVLIAGHFWDPRSPVFFTKEDVE
                     KPDFRISVIGDISCDIGGPIPTTLRATTIADPFYSYNRKKHSEEDAFSNPDNITVMSI
                     DNLPGELPRDASGDFGKQLIDNVLHDLFTGHDSPMIQRATITLGGRIEPQYGNLTGWV
                     WPEGEKP"
BASE COUNT          442 a          535 c          506 g          488 t
ORIGIN      
        1 cctgtttcga gctggtggat gagatcaaag taaggttttt cgctctcgta gaactcaccc
       61 gcaacgataa gcttcacgcc gagatcgcga acacactgct cggccatggc cctcaggagc
      121 aggtcaagtc ccttgtactc ccttataaac ccgaagaaga ggatgaattt gtcagccgga
      181 cacagtttga gacgtacgat tgcctcagcc cgggacaccg gaacgctgta gttatcgaac
      241 agaggatgcg gagagatcac gcagggcata tcagcagcaa actgcctgag gtcatcctcc
      301 actgccctgg tcatcaccaa tgcgccgtca atgctcttca caaagaaccg ggtgagcatc
      361 gtgtctccga accgtttctc gtggggcacc acattatcga aaatggtgat gaccctggtg
      421 tgcccgttac tccttacaag ccgggcaatg aaccccagcg ccggggcgag gaaaggaagc
      481 cagtaccgca ttattacaag gtccggccgg gtgcgtctga gacgaagccc gatattaaac
      541 cagttcagcg gattgaccga gttaatggct cttgtaatag gaaccccctc cggggcgtac
      601 ccatggtcat actgtgtctt ccccgggaag agaaaagagg ggtactgaag tttgaatgta
      661 tatattgcag cagcatcacc ttcctgaagc agctgctgtg ccagcttctc gttgtaggcg
      721 gctatcccgc cacggtacgg atgggcgggg ccgcagatga cgatccgcat catggcttct
      781 caccttcggg ccatacccag cctgtcaggt tcccgtactg cggttcaatc ctcccgccca
      841 aggtaattgt ggcgcgctga atcatgggtg agtcatgacc tgtaaaaaga tcatgcagca
      901 cattatcaat caattgcttg ccgaaatcac ccgaggcatc cctcggcagc tcaccgggaa
      961 ggttgtcaat tgacatgact gtgatattgt caggattgct gaatgcatcc tcctcgctat
     1021 gctttttccg gttatagctg tagaacgggt ctgcaatggt ggtggcccgc agggtggtgg
     1081 gtatcggacc gcctatatcg cagctgatgt caccaatgac cgatatcctg aaatcaggct
     1141 tctcaacatc ctccttcgtg aagaagacag gtgaccgggg atcccagaag tgtccggcga
     1201 tcagaacatc cgttaccttg gtgtatttca ggaaatcaga ccggtaatcc tcaggatgct
     1261 gtacgaaatt gttgaagtca aagggacggc cgtcatgatg cttcacgtaa tgctgcggac
     1321 ccacctggca gacaacggga acatcgtact cctttgacag gaagtcttcg atgcttacct
     1381 tcagcaggtt attgcagttg gccagggtct ccatggcgcc gtttgccacc ctgccctcgc
     1441 ctgtgaccag gatcttcagt cccggtttga gctgtatcag cctcagtccg gcccacatct
     1501 cattcaggtc atgacactgg taggctggtt tcagcttgaa cctgtttgtt tttatacccc
     1561 tggctctcag ggcattatat gcgccaacaa tacctgccca gcggccgaag gccaccactc
     1621 tctgaccctt gtccgtagtg aggtactcgt agtcaataac cttcactttc cggctgagta
     1681 tttccctgag catctcgagg ttatgttcct gtttcttggc cacatgggca aagaagaggt
     1741 atgtctttcc ggggatgaat gctctcttgt ccacctcctt gactcccata aggatatcac
     1801 agttactgag atcttcccga atgggaatct tcaggtattt gtattcgtcg tcagtgaagc
     1861 acctgatatc tgacggctgg acataaaatt ccacgtcggg atatttatct ttcagttcca
     1921 ctacctgtga cggtgtcagc ggcacccttc tgtctggcgg tatctttgtc t
//
LOCUS       DAOPXU010000214         1912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_90318_length_1912_cov_9.007001, whole genome shotgun sequence.
ACCESSION   DAOPXU010000214 DAOPXU010000000
VERSION     DAOPXU010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90318_length_1912_cov_9.007001"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /locus_tag="PKK03_12510"
     CDS             <1..1536
                     /locus_tag="PKK03_12510"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNX85261.1"
                     /translation="LALFAMYLVWLLNTTDFHGALAELRLKLPLLFFPVVAGTTERTD
                     RSLLRMVLLSFIAGCLFATVAGFAALAGLMPGEIRSSRELALFVPSIRLSILLCFAIF
                     SSLWLIFSDGGEERQAGTDGSTSADSDDITSAGGSHLPAWWQRLTARLLPGTGGRLYK
                     MMFAAAAAAMSYFLFRLLSVTGLVIFVLLLFLTGIFLITAGRRVRSGITFLAAAAAAT
                     AALTILSVSAWNRLHNPADPSLNGKRQFTLSGRAYIHYPEETLMENGYLVWVNVCEEE
                     LRQEWNRRSLIAYDGADAAGNELRVTLVRYITAKRMPKDSAAVASLSDSDIFNIEQGY
                     ANPLYARKGSPMARAYEIAWEIDRAAGGANPSGHSLTQRPEFYRAAAGIISKHPWTGV
                     GTGDLARAFSDEYASISSPLKPEYRLRAHNQYLTFGVTFGIPGMILAVALLLIPWLMS
                     GGRFSYLFLVFMTVILISMFNDDTFSSFTGAAFFSYFYTLLIVTSAGKVSIHPPRQRK
                     EEC"
     gene            1555..>1912
                     /locus_tag="PKK03_12515"
     CDS             1555..>1912
                     /locus_tag="PKK03_12515"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNX85262.1"
                     /translation="MRLNSKDRKFLELAIKTAGENMEDGGGPFGAIITRGEDILARAG
                     NRVVPGHDPTAHAEVMAIRMAAERLGTHDLADCTIYASCEPCPMCLGAIYWAGIRRLV
                     YASDRYSAASAGFSDNH"
BASE COUNT          399 a          516 c          533 g          464 t
ORIGIN      
        1 cttgcactct tcgcgatgta cctggtgtgg ctgctgaata caaccgactt ccatggagcg
       61 cttgctgagc tcaggcttaa gctcccgctg cttttcttcc ctgttgtggc cggcacaacc
      121 gaaaggacag acaggtctct tctccggatg gtcctgctct ccttcatcgc cggatgtctg
      181 ttcgccactg ttgccggttt cgccgcactt gccggtctga tgcccggaga gatacgctct
      241 tcgcgcgaac ttgctttgtt tgtaccgtca atacggctat caatacttct ctgcttcgcg
      301 attttttcct ctttgtggct tattttttct gatggagggg aggagagaca ggccggcaca
      361 gatggtagca cctctgctga ttcggatgac atcacctccg ccggcggatc acatttgccg
      421 gcctggtggc agcgtctgac tgcccggctt cttcccggca ccggcggaag actgtacaag
      481 atgatgtttg ccgccgcggc cgcagccatg agctatttcc tgttcaggct tctttcggtg
      541 acagggcttg tcatattcgt gctgctgctc ttcctgacgg ggattttcct gatcactgcg
      601 ggcaggcgcg tgcgttcggg cataaccttc ctggctgcgg ccgcggcggc aaccgctgca
      661 ttgacaatcc tgtcagtcag cgcctggaac aggctccata atcctgccga tccatcgctg
      721 aacgggaagc ggcaattcac attgtctgga agggcatata tccactaccc tgaggagacc
      781 ctgatggaga acggctatct ggtctgggta aacgtgtgcg aggaggaact gaggcaggag
      841 tggaaccgga ggagcctgat agcatatgac ggcgccgatg cagcaggcaa tgagctgcgc
      901 gtcaccctgg taagatacat caccgcaaag agaatgccca aggactcggc agcagttgca
      961 tcgctttcag acagtgacat tttcaatatt gaacaggggt atgcaaatcc tctttatgcg
     1021 aggaagggta gccccatggc cagggcatat gagattgcgt gggaaattga ccgtgcagcc
     1081 ggtggcgcca atccctcagg ccactctctt acgcagcggc ctgaattcta ccgggcagcg
     1141 gccggaataa tcagcaaaca cccctggaca ggagtgggga caggtgatct ggcccgtgcc
     1201 ttcagtgatg agtatgcatc aatctcctcc ccgttaaagc ctgagtaccg tctgagggcg
     1261 cataaccagt atctgacatt cggtgttacc ttcggcattc ccgggatgat actggccgtg
     1321 gccctgctgc ttatcccatg gctgatgagc ggtggccgct tctcatacct gtttctggtc
     1381 ttcatgacag tcatcctgat ctcaatgttt aatgatgata cattcagttc ttttaccgga
     1441 gcggcattct tttcttattt ttacaccctg ttaattgtca cttcagccgg aaaggtaagt
     1501 attcatcctc cccggcagcg aaaggaagaa tgttaattgt cacatttgac ggtaatgagg
     1561 ctcaacagta aggacaggaa attccttgaa cttgcaataa aaacggcagg ggagaatatg
     1621 gaagatggcg gcgggccatt cggtgcaata atcacaaggg gcgaagacat ccttgcccgg
     1681 gcaggcaacc gggttgttcc gggacatgat ccgacagccc atgccgaggt gatggccata
     1741 aggatggctg cagagagact tggcacgcat gacctggctg actgcaccat ttatgcttca
     1801 tgcgagccct gtcccatgtg cctgggagcc atttactggg cagggatcag gcgccttgta
     1861 tatgcctccg acaggtacag cgccgcctcc gccggattca gtgacaatca ta
//
LOCUS       DAOPXU010000215         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_95189_length_1849_cov_12.842252, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000215 DAOPXU010000000
VERSION     DAOPXU010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95189_length_1849_cov_12.842252"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..624)
                     /gene="trxA"
                     /locus_tag="PKK03_12520"
     CDS             complement(208..624)
                     /gene="trxA"
                     /locus_tag="PKK03_12520"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNX85263.1"
                     /translation="MKGLIIALVVIAVVAILLVRSYNKMKNAPAVADSELIEHLTAQN
                     FAHKTKNGVVLVDFWADWCMPCKMMAPILNEVAEATDGTATIYKLNVDEQQQVAAQYG
                     IRSIPTMILFKDGKEVERIVGVKSKEAVIASINKVQ"
     gene            complement(635..1573)
                     /locus_tag="PKK03_12525"
     CDS             complement(635..1573)
                     /locus_tag="PKK03_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX85264.1"
                     /translation="MENVNHYDVIILGGGPAGLTAAIYLSRARLSTLLLTEGTPGGQM
                     TLTHEIANYPGIEKINGYMLGSVMKKQATAFGAVVKGNTEISSFCLEGEVKSVTAGDG
                     QTYTAGAVILATGGRSRTLGVPGEDFFKGRGISYCATCDGDFFTGKEIVVVGGGNSAL
                     EEAVSLTKYATKVTVVHQFDHFQAFQYAVDEAKANPKLSFVMSSTIASFYGEEKLEGV
                     KIRNLVTGEDRDFKTAGVFIFIGYVPNTEWLRGYVNMNDRGEIITGADMSTNVEGVFS
                     AGDANAKRYRQVTTAVADGTVAALAAADYQHSQKRK"
     gene            complement(1577..>1849)
                     /locus_tag="PKK03_12530"
     CDS             complement(1577..>1849)
                     /locus_tag="PKK03_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNX85265.1"
                     /translation="CCGRMGPMCIPVYGIMEELEPVYTHVKCFDMEFDNPEAQVIRNA
                     PLTRGFMGLPYTVYYRNGKMVKATSSIQTMQQVKTILESEFGKPNA"
BASE COUNT          399 a          535 c          426 g          489 t
ORIGIN      
        1 tattttgatt cctttggaag agtaagaccc tctgagcggt tcaatcaact acttaaggaa
       61 cccgactatg aaagggcacg aaagggattc gacaggtaat gcctgccaaa cccctccgtc
      121 caattgacac aagaacttcc ggtctgtctg accggcctga ttgttcaccg gttccggtct
      181 gctgtccaca ccagccggcc cggtgtatta ctgaaccttg tttatgctgg caataaccgc
      241 ctccttcgat ttcactccta ctatcctctc cacttccttg ccatccttaa acaggatcat
      301 cgtcgggata ctcctgatcc cgtactgcgc agctacctgc tgctgttcat ccacattgag
      361 cttatatatt gttgcagtgc cgtcagtagc ttcagccacc tcgttaagga tgggcgccat
      421 catcttgcac ggcatgcacc agtcggccca gaaatcaacc agtacaactc cattcttcgt
      481 cttgtgcgcg aagttctgtg ccgtgagatg ttctatcagc tcactgtcgg ctactgccgg
      541 ggcattcttc atcttgttgt acgagcgcac aagaagtatt gcaactacgg caataacaac
      601 caaagctata atcagtcctt tcatcttcat ctacttattt tcttttttgt gaatgctggt
      661 aatcggcagc agcaagcgct gcgacggttc cgtcggcaac agccgtggtc acctgcctgt
      721 aacgttttgc gttggcgtca cctgcagaaa aaacgccttc gacatttgtt gacatatcgg
      781 ctccggtgat gatctcaccc ctatcgttca tgttcacata ccccctgagc cactcagtgt
      841 tgggcacgta accaataaag atgaagacac cagcagtttt gaaatcccta tcctcgcccg
      901 tgacaaggtt cctgattttt acaccttcca gtttctcttc tccgtaaaac gaagcgatgg
      961 ttgatgacat cacgaagctg agtttcgggt tggccttcgc ctcgtcaacg gcatactgaa
     1021 atgcctggaa atggtcgaac tggtgaacca cagtcacctt tgtggcgtat ttcgtaagtg
     1081 agacggcttc ctcgagagcc gagttgcctc cgcccacgac aactatctcc ttgcctgtga
     1141 agaaatctcc gtcacaggtt gcacagtatg atatgcctct tcccttgaag aaatcctcac
     1201 cgggcacgcc gagggtgcgc gaccggccac cggtggcaag gattactgca cccgcagtgt
     1261 atgtctgacc gtctccggca gtgacactct ttacctcgcc ctcaaggcag aacgagctga
     1321 tctccgtgtt gcccttcacc acggcgccaa aggcagttgc ctgtttcttc atcaccgagc
     1381 cgagcatata tccgtttatc ttctcgatgc ctggatagtt ggcaatttcg tgggtcagag
     1441 tcatctgtcc tccgggagta ccctcggtga ggagcagtgt gctcagcctg gcccgcgaga
     1501 ggtagattgc cgctgtcagc cctgccggac caccgccaag aatgattaca tcgtaatgat
     1561 ttacgttctc cattgcctat gcgttgggct tgccgaactc actttcgagg attgtcttca
     1621 cctgctgcat cgtctggatg ctggatgttg ccttcaccat ctttccattc ctgtaataca
     1681 cagtgtatgg caaccccatg aatccgcggg taaggggtgc attgcgtatc acctgtgcct
     1741 ccggattgtc aaactccatg tcgaaacatt tcacatgggt atagacgggt tcgagctcct
     1801 ccatgattcc gtaaaccggg atacacatcg ggcccatcct gccgcagca
//
LOCUS       DAOPXU010000216         1745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_104370_length_1745_cov_9.852071, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000216 DAOPXU010000000
VERSION     DAOPXU010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104370_length_1745_cov_9.852071"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PKK03_12535"
     CDS             <1..590
                     /locus_tag="PKK03_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85266.1"
                     /translation="LQRTFEAGYYIHHEEQQALFDYRFRSNQSLISHCNDSSALPILK
                     ELNDRTVELISVINNTEARLIAEAEGKPGVPAILTNQMAKTETGPVIQFKAIKQPFHT
                     APFRNFLMKDSGLRNELAAALKKYSDYLSDQLPADKFEMYAKLLEPSVYLQEVNPETD
                     RIPLITGLHMLELLKNSILAAEAYAIETVSGTNNQ"
     gene            602..1540
                     /locus_tag="PKK03_12540"
     CDS             602..1540
                     /locus_tag="PKK03_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85267.1"
                     /translation="MKQKIYTLGLIITSVIFLGALLKINHWPGGGHLLTLGIVTLVVV
                     FLPLALRNHYRTEGGRKNLLLYIVTWVTCLVMFGGMLFKLMHWPGAGKALLIALPFPY
                     VVFLPVFLAVTSKDKNFSIHNTVAVLFLLAGTSISSALLSLNVSKERLDDSMRLSRSY
                     NRIEAVLDEIPSAAGHTTAINKIDELLGIIDDYQRRIFSIEGMSEEDWNNDPWTFPFP
                     EKTSIADKTLFIGGKYPTYDTRLQTGLGELAGELQKSAANEEIAGKIPLILGFYETPG
                     DNLGWTQGMFVVNPRVWSLIYLDGLETNLKLIRAGI"
BASE COUNT          476 a          400 c          412 g          457 t
ORIGIN      
        1 gcctgcaaag gacttttgag gcaggttact acatacatca tgaagaacaa caggctctgt
       61 ttgattacag gttcaggtca aatcagtcat tgatcagtca ctgcaatgac tcctctgctt
      121 taccaatcct taaggagcta aatgacagga ccgttgaact gatatcagtc atcaacaata
      181 ccgaagccag gctcattgcc gaagctgaag gaaaacccgg ggtgccggca atactgacca
      241 atcagatggc aaaaactgag accggacctg taatacagtt caaggcgata aagcagccgt
      301 tccatactgc tcctttcagg aacttcctga tgaaggattc aggcctgcgg aatgaacttg
      361 ccgctgccct gaagaaatac tctgattatc tgtcggatca gttgcctgca gataagtttg
      421 aaatgtacgc gaagcttctt gaaccttccg tttacctgca ggaggtaaat cctgaaaccg
      481 acaggatccc gctgataacc ggactgcata tgttggaact gctgaaaaac agcatccttg
      541 ctgcagaggc atatgcaatt gaaacggttt cgggaactaa taaccaataa aacttaagga
      601 tatgaaacag aagatatata ccctggggct tatcattacg tcggtaatct ttcttggtgc
      661 tctgcttaag ataaatcact ggcccggcgg agggcatctt ttgactcttg gtattgtcac
      721 gctcgtcgtg gtattccttc cgctggcttt gagaaatcat tacaggactg aaggcgggag
      781 aaagaatctg ctgctttaca ttgtaacatg ggtcacatgt ttagttatgt tcggtggtat
      841 gcttttcaag ctgatgcact ggccgggagc gggaaaagcc ctgctgatag ccctcccctt
      901 cccgtatgtt gttttcctcc cggtttttct tgctgtgaca tcaaaggata agaatttcag
      961 cattcataat actgttgctg tacttttcct tcttgcaggc acgtcaatct cctctgcatt
     1021 gctgtcgctc aatgtttcga aggagaggct tgatgacagc atgcggttgt cgcggagtta
     1081 taacaggatt gaggcggtac ttgatgagat cccttctgct gcgggtcaca caacggctat
     1141 aaacaaaata gatgaacttc tgggcattat tgatgactat cagcgcagaa tattctccat
     1201 tgagggtatg tcggaggaag actggaataa tgatccatgg acattcccgt tcccggaaaa
     1261 aacatctatc gcagataaaa ccctgttcat cggagggaag tatcccactt atgacacaag
     1321 gctacagacc gggctgggag aactcgccgg cgaacttcag aagtctgccg ccaatgaaga
     1381 gattgccggc aaaataccat taatccttgg cttctatgag acgcccggtg acaacctggg
     1441 gtggacgcag ggtatgtttg ttgtaaatcc ccgcgtgtgg tcacttattt atcttgacgg
     1501 cctggaaaca aatctgaagc tgatcagggc aggaatctga ccttgtgaat aaaagccatc
     1561 ttccggatct gaagatatca tacatcttac aaaatacatc ctgacagcct catcccatga
     1621 gggtttgcgg aggttttggc acaccctttg cattacctcc attgaagagc gatcttcata
     1681 aaacgtaaaa agatgaaaaa gtcagttatc acatcggtcc tcgcagttct ggcagcctta
     1741 acgac
//
LOCUS       DAOPXU010000217         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_104450_length_1744_cov_9.012433, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000217 DAOPXU010000000
VERSION     DAOPXU010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104450_length_1744_cov_9.012433"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..414)
                     /locus_tag="PKK03_12545"
     CDS             complement(109..414)
                     /locus_tag="PKK03_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85268.1"
                     /translation="MKYTARHIQRFLETGKIDVTLWKIHFPLLLPSQVPSCEECSEFK
                     DHTCAGGREPVDCFLAKTRELIPDEKQKQKKRDPMLGLGAREPALRGTAQPRDQSKM"
     gene            853..>1744
                     /locus_tag="PKK03_12550"
     CDS             853..>1744
                     /locus_tag="PKK03_12550"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNX85269.1"
                     /translation="MKYSVLIIAALLITAALIAGCSQSQSATQQPSGTASTGVIKVGV
                     IASLTGPASNVGTNMWQSAQVAAEKINADGGITLKDGSKAQLRLIVGDDESTQQGGQK
                     AATKLITDDKVDILVGGYSSAVTSAYEQTIAEYKVPYIVTGASSPIITHRPDIDTSYI
                     FHHCPTTDSYGQYTTQFIDQVIRPAVNKKLGVADSRPFRLALLYQDTAFGKGIQTAVN
                     DTITKNKLNIVLVSQQSFRMGESDFRTQLTAIKAANPDAVYLGAFPNEGAPAITQARR
                     DISLNTIFLSAENNDNAQFYK"
BASE COUNT          439 a          477 c          430 g          398 t
ORIGIN      
        1 ttcactgttg gatacagggc atcttcactt tttacagcac ttcgtgtaca ctacaaatca
       61 gcccaggtgg taacttctca attgactgaa tataattacg tccggaactc acatctttga
      121 ctggtccctg ggctgggcgg ttccccgaag ggccggttcc cgggcaccaa gaccgagcat
      181 gggatcccgt ttcttctgct tctgtttttc atccgggatt aattcccgag tctttgcaag
      241 gaaacaatcg accggttccc ggccaccggc acaggtatgg tccttgaact cgctgcattc
      301 ttcacaggat ggcacctgag atggcagcag cagggggaaa tgaattttcc agagcgttac
      361 atcaatcttt cccgtttcga ggaaacgctg gatatgccgt gccgtatatt tcatgccggc
      421 agatatgggc agtgagggga tttagggttt gtggatgccg gaagtcatca gacctgagac
      481 tggagaagaa cccctcaggg tgcttcgact tgtcaccaga cccggaacgg agaatgctga
      541 atattcataa gacgcaaaga ggagaaggcc cgacataatc ctgtgaacaa atcacagatt
      601 ttcggccatc taactaagag ttcttcgatt tgccgcccga cccggtacaa gaatatacct
      661 tatcagggat cttaaagtgc accgcaggtc ttcaatccgg gaattcggtc catgcgtcag
      721 tcactctggt tggctgactg caccacatct gctgtcatgg tgcaccactt ccgcgaaaaa
      781 tccaatattt tttatatagt cagccgccca tcacatatat gccatgctat atcatagcat
      841 tgaggagatc ccatgaaata ttctgtcctt atcatagcgg cactgctcat cactgcggca
      901 ctcattgccg gctgttccca gtcgcagtcg gcaacgcagc aaccttccgg cacagcatca
      961 acgggtgtca taaaggttgg tgtcattgca tcgctgacgg gccccgcaag taatgtcgga
     1021 accaacatgt ggcagtccgc gcaggttgcc gctgaaaaga tcaatgccga tggcggcatt
     1081 actttaaaag acggatcgaa agcccagctc aggctcattg ttggcgacga tgagtcaacc
     1141 cagcagggag gccagaaagc ggcaacaaaa ctgatcaccg atgacaaggt agacatcctt
     1201 gtcggcggat actcgagtgc cgtcacctcc gcctacgaac agactattgc tgagtacaag
     1261 gtcccgtata tcgtcacggg tgcatcaagc ccgatcatca cgcaccggcc tgatatcgat
     1321 accagttaca tcttccatca ttgcccgaca accgatagtt acgggcaata cacaacacag
     1381 tttatcgatc aggttatccg gcctgcggtt aacaagaaac tcggcgttgc agacagccgc
     1441 ccgttccggc ttgccctgct ttaccaggac acggctttcg ggaaaggtat ccagaccgca
     1501 gtgaatgata cgatcacgaa gaacaagctc aatattgtcc ttgtctccca gcagagcttc
     1561 aggatggggg agagcgattt ccggacccag ctgactgcga tcaaggcagc aaaccctgac
     1621 gctgtgtatc tcggtgcgtt ccccaatgaa ggagcccctg cgatcacgca ggcacgcagg
     1681 gatatcagtc tcaacaccat cttcctctcc gcagagaaca atgacaatgc ccagttctac
     1741 aagg
//
LOCUS       DAOPXU010000218         1708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_107908_length_1708_cov_12.188143, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000218 DAOPXU010000000
VERSION     DAOPXU010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107908_length_1708_cov_12.188143"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1708)
                     /locus_tag="PKK03_12555"
     CDS             complement(<1..>1708)
                     /locus_tag="PKK03_12555"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNX85270.1"
                     /translation="GASCDWDRTLFTMDEPRYISVIKVFVDLYNKGLIYRGVRMVNWD
                     PQALTAVSDEEVVYTEEKSKLYYVRYRIVGEDNYIMVATTRPETILGDMAVCANPNDE
                     RYAHMKGKKVIVPMANREVPFIFDEYVDMEFGTGCLKITPAHDINDYEIGLKYNLKSI
                     DIFNDNGTISEAAGMFTGKDRFEARVLADEELRRTGALVKVEDYINKIGRSERTHAVI
                     EPKLSLQWFLKMKDISKPALDAVMDDTVRLYPDKFRNVYRHWMENVHDWCISRQLWWG
                     QRIPAWYYNNNEFVVAVTEDEALEKARNITGRADLTMNDLRQDDDVLDTWFSSWLWPI
                     TCFDGINNPDNEDIKYYYPSNVLVTAPDILFFWVARMIIAGYEYRGREPFGDVYLTGM
                     VRDKQKRKMSKSLGNSPDPIGLIDRYGADGVRVGMLLCSPAGNDLLYDDSLPEQGRNF
                     ANKIWNAFRLVMSMSPDDSVEQPAASKAAVTWMRNTIDKTLAEIEDNFVRYRISDALM
                     QAYKLFWDDFSGWYLEIIKPAFGKPIDGETRRETLAIFGDLMKILHPFMPFITEEIWQ
                     RLA"
BASE COUNT          397 a          467 c          420 g          424 t
ORIGIN      
        1 cagccagcct ctgccatatc tcctcggtga taaagggcat gaagggatgc agaatcttca
       61 tcaggtcacc gaagatggcc agcgtctcac ggcgggtctc tccgtcaatg ggcttgccga
      121 aggcgggctt gatgatttca agataccagc cggagaaatc atcccagaag agtttgtaag
      181 cctgcatcag tgcatcggag atgcggtagc gtacaaagtt gtcctcgatc tccgcaaggg
      241 ttttgtctat ggtattgcgc atccaggtga cagccgcctt cgaagccgcc ggctgttcaa
      301 cgctgtcatc aggactcatt gacatcacaa gccggaaggc attccatatc ttgttggcaa
      361 agttgcgccc ctgctccgga aggctgtcgt catacagcag gtcattcccc gcaggggaac
      421 agagaagcat gccaactctc acaccgtcag ccccgtacct gtctatgagc ccgatgggat
      481 cgggagagtt gccgagcgat ttcgacatct tgcgcttctg cttgtcgcgt accatgccgg
      541 taaggtatac atcaccaaat ggttcgcgac cgcggtactc atacccagcg atgatcatcc
      601 tggcaaccca gaagaaaagt atgtccggag cggtcacaag gacattggag ggataatagt
      661 acttaatgtc ttcgttatca gggttattaa tcccgtcaaa gcaggttatg ggccatagcc
      721 atgatgagaa ccatgtgtca agcacatcat catcctggcg aaggtcattc atggtcaggt
      781 ctgccctgcc ggtgatgttg cgggcctttt ccagcgcctc atcttcagtc acggcaacaa
      841 caaactcatt attgttgtaa taccatgccg ggatccgctg accccaccac agctgcctgc
      901 tgatgcacca gtcgtgcaca ttttccatcc agtgcctgta aacatttctg aacttgtcag
      961 ggtaaagcct gacggtatca tccatcaccg cgtcgagggc cggtttggag atatccttca
     1021 ttttaaggaa ccactgcagt gagagcttcg gttcaatcac cgcatgggtg cgctctgacc
     1081 tcccgatttt atttatgtaa tcttcaactt ttacaagagc gccggtacgg cgaagctcct
     1141 catcagcaag gacgcgggcc tcgaaacggt ctttgcctgt gaacatgcct gccgcttctg
     1201 agatggtgcc gttatcattg aaaatgtcaa tactcttcag gttatacttc aggccgatct
     1261 catagtcgtt tatgtcgtga gccggggtga tcttcagaca ccccgtccca aactccatgt
     1321 caacatattc atcgaagatg aacggaacct cccggttggc catgggtaca atgaccttct
     1381 tccccttcat gtgtgcatag cgctcatcat tcgggttagc gcatacagcc atgtcaccaa
     1441 ggattgtctc aggtctggtg gttgccacca tgatataatt gtcctcacca actatcctgt
     1501 agcgcacata ataaagtttc gacttttctt cggtgtagac aacctcttcg tccgagacgg
     1561 ccgtgagagc ctgcggatcc cagttgacca tgcgtactcc gcggtagatc aaccctttgt
     1621 tataaagatc gacaaagacc tttatcaccg atatgtaacg gggttcatcc atggtgaaca
     1681 gggtgcggtc ccagtcacat gacgcccc
//
LOCUS       DAOPXU010000219         1700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_108717_length_1700_cov_10.153191, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000219 DAOPXU010000000
VERSION     DAOPXU010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108717_length_1700_cov_10.153191"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PKK03_12560"
     CDS             complement(<1..307)
                     /locus_tag="PKK03_12560"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNX85271.1"
                     /translation="MTKRILIFATLPLLLLASCSPKPKEQWIPLFNGRNLDGWIIKIK
                     GSPLGENYKNTFRVEDSVMKVSYSEYDTFRYEFGHIYYKEPFSSYKLRVEYRFTGEQA
                     "
     gene            complement(408..>1700)
                     /locus_tag="PKK03_12565"
     CDS             complement(408..>1700)
                     /locus_tag="PKK03_12565"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNX85272.1"
                     /translation="NRFKDKVWRLLGRYKRVGTRARILAAQLESGIKGTVLEQSNMFE
                     ALKFRYFGPIDGHDVLHLTRVLEDLKTIPGPKLLHCVTVKGKGFAKAEEQQTIFHAPG
                     KFDKCTGELIRPAECPKHPLYQEVFGHTIVELARQNEKIVGITPAMPSGCSLNIMMKE
                     MPDRAFDVGICEQHAVTFSAGLAANGLLPFCNIYSSFMQRAYDQVVHDVAIQGLKVVF
                     CMDRGGLVGTDGATHHGFFDIAFMRNIPGMITSAPMDEVELRNMMYTAQLDSIKGPFS
                     IRYPRGTGVICDWRQPFEEIPVGRARLIGEGSDIAVLSLGHPGNFVVEAAGRIREKGI
                     SVLHYDMRFANPLDEEVLHSVMKRFKKIITVEDGMIKGGFGSAVLEFANDHGYAVEVR
                     RLGIPDYFVEHGSQQELWHECGYDAAGIEQAIVQAAGH"
BASE COUNT          400 a          460 c          417 g          423 t
ORIGIN      
        1 gtgcctgttc accggtgaaa cggtattcaa cacgcagctt gtatgatgag aacggttcct
       61 tgtaatagat gtggccgaat tcatacctga aggtgtcata ctctgaatat gagaccttca
      121 tgacactatc ttcaaccctg aatgtgtttt tgtagttttc tccaaggggt gatcccttga
      181 tcttgatgat ccagccgtcg aggttccggc cgttgaagag ggggatccat tgttccttcg
      241 gcttcggcga acaggaggcg aggaggagga gggggagggt ggcgaaaatc agaattcttt
      301 ttgtcatgga ttacaatgtt gaatgcccta cgggcaatgt tataagatca ggttctgtgt
      361 tacacgctcc attaaatcgc aaatcgcaaa tcgcacgtct gcaattctta atggcctgct
      421 gcctgaacaa tggcctgctc aataccagcc gcatcatacc cgcactcatg ccagagctcc
      481 tgctgggatc cgtgctcgac gaagtaatca ggtatgccca ggcgtctgac ctcaaccgca
      541 taaccgtgat cattcgcaaa ctcaagtacg gcgctgccaa atccgccttt tatcatgccg
      601 tcttccacgg tgatgatctt tttgaatcgc ttcatgacac tgtgcagcac ctcctcgtca
      661 agcgggttgg cgaaacgcat atcatagtgc aatacagaga ttcccttttc acgaatccgc
      721 ccggcagctt caacaacaaa atttcccgga tgtccaaggc tcagcacagc aatgtcagat
      781 ccctccccga taagccgggc gcgtccaacc ggtatctcct caaaaggctg gcgccagtca
      841 cagataactc ctgttccgcg gggataacgg atgctgaaag gtcccttaat actgtcaagc
      901 tgggcagtat acatcatgtt acgcagttca acctcatcca tcggcgctga ggtgatcatc
      961 ccggggatgt tgcgcatgaa tgcgatgtca aagaaaccgt ggtgtgttgc accgtcagtg
     1021 ccgaccagtc ctccccggtc catgcaaaat accaccttaa gtccctggat ggcgacatca
     1081 tgcaccacct gatcataagc gcgctgcatg aatgacgagt aaatattgca gaaggggaga
     1141 agcccgtttg ccgccagtcc tgccgagaag gtgactgcat gctgctcaca aatcccaacg
     1201 tcaaatgccc tgtcaggcat ctccttcatc attatgttca gcgaacaacc tgagggcatg
     1261 gcgggggtta ttcccactat tttttcattc tgccgggcca actctacaat tgtatgaccg
     1321 aagacctcct ggtagagcgg gtgtttgggg cattcggccg gtctgataag ttcaccggtg
     1381 catttgtcaa atttgccggg ggcatgaaag atagtctgct gttcctcagc tttcgcgaat
     1441 cctttcccct tcaccgtgac acagtgcagc agtttgggac ccggaatggt cttaagatct
     1501 tcaaggaccc gggtcagatg aagtacgtca tgcccgtcaa tcgggccgaa atacctgaat
     1561 ttcaatgcct caaacatgtt gctctgttca agtacagtac ccttgatgcc gctctcaagc
     1621 tgtgctgcaa ggatcctggc cctggtacca accctcttgt agcggccgag caggcgccat
     1681 accttatcct tgaacctgtt
//
LOCUS       DAOPXU010000220         1696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_109105_length_1696_cov_10.243754, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000220 DAOPXU010000000
VERSION     DAOPXU010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109105_length_1696_cov_10.243754"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PKK03_12570"
     CDS             complement(<1..187)
                     /locus_tag="PKK03_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85273.1"
                     /translation="MKATKLLLLIAAAALTFMVSCKGVEEPEVIPALYFMDTSDEIIG
                     KIVLDGTNTLSTVKDVAG"
     gene            complement(218..1549)
                     /locus_tag="PKK03_12575"
     CDS             complement(218..1549)
                     /locus_tag="PKK03_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX85274.1"
                     /translation="MKKALYIIGILAILTGCEVLNVEPEDAIPAGEAFKDKAGIEKGI
                     LGSYSSLQYLSYYGRTYLIFSDLAADNLSHPTDATNSDYAEVDNNAILAENSSISGIW
                     AACYDGINVANNVIVKVPEMADMTDTEKNRALGELYFLRALGHFNLVNYFGAIPIKIE
                     PTVGVSNLDAGRDPVNEVYAQIRSDLVLASKYLQPSVSTKTRASKYAALALLARLSLY
                     SGDYAMAREKADSVIKYGGYTLPGNYADVFSSDGSAETIFEIDFTELDRNRIAEHNFP
                     KTLNGRREVEPDASLITAYEAGDERKAVSIAYDGALAYANKYNDLSKGADNLIVLRLA
                     EMYLIRAEAEARLTGGNITAVRSDINAIRSRADLGPTGAVTADEMLLAIEQERRVEFA
                     FEGHRWFDLVRTGRALEVLPNVTRANQTLFPIPADEILTNNSPEMIQNPGY"
BASE COUNT          390 a          425 c          413 g          468 t
ORIGIN      
        1 ttcctgccac atccttgaca gttgagagag tattggttcc gtcgagaact attttcccga
       61 tgatctcgtc agaagtatcc atgaagtaaa gtgccggaat tacttcaggt tcttctactc
      121 ccttgcacga aaccataaag gtcagtgccg cagcagcaat cagtaaaagg agttttgtag
      181 ctttcattgt catgtgtttt agtttatcat caagatttta atatccggga ttttgtatca
      241 tttcaggact gttgtttgtc aggatctcat cggcagggat gggaaacagt gtctggttgg
      301 ccctggtgac attgggcagt acttccagtg ccctcccggt ccttaccagg tcaaaccacc
      361 ggtgcccctc gaaagcaaat tccacccgtc tttcctgctc aatggccagt aacatttcat
      421 ctgcagtcac agcacctgtg gggccaagat cagccctgct ccttatcgca ttgatatccg
      481 acctgactgc tgtgatattc cctccggtca gtctggcttc agcctctgcc ctgatcaggt
      541 acatctctgc caggcgcagg actatgaggt tatcggcacc cttgctcagg tcattgtatt
      601 tgttggcata tgccagggcc ccgtcatagg ctattgagac tgctttccgt tcgtcacccg
      661 cttcataggc agtgataagg cttgcatcag gttccacctc tcttctcccg ttaagggttt
      721 tcgggaaatt gtgctcggca attctgttcc ggtcgagctc ggtgaaatca atctcgaata
      781 tcgtctctgc tgatccgtca gatgagaaaa catcagcgta gtttcccgga agggtgtaac
      841 ctccgtattt gatcacactg tctgcctttt cgcgggccat cgcataatca cccgaataaa
      901 gggagagtct tgcaagaagg gcaagggcag catatttcga tgccctggtt ttggttgata
      961 cggaaggctg caggtattta gaggccagca caagatcaga cctgatctgg gcatacactt
     1021 catttaccgg gtcacgcccg gcgtcaaggt tgctgacccc taccgtgggt tcgatcttta
     1081 tgggtatggc cccgaaatag ttcacgaggt taaaatgtcc cagggcccgg agaaaataaa
     1141 gctcacccaa cgccctgttt ttttcggtgt cggtcatgtc agccatctcc ggaactttga
     1201 caatcacatt gttggcaacg ttgatgccgt catagcaggc agcccagatc ccgcttattg
     1261 aagagttttc tgcaagtatg gcgttgttgt caacctcagc gtagtcagaa ttggtggcgt
     1321 ctgtagggtg cgacaggtta tcagcggcga ggtcagaaaa aatcaggtaa gtcctgccgt
     1381 aataactcag gtactgaagc gaggagtaac tgcccagtat gcccttttca atcccggcct
     1441 tatccttgaa tgcctcacct gcagggatgg cgtcttcagg ttcaacgttc agaacctcac
     1501 atccggtcag gatggccagt ataccgatta tatacaatgc tttcttcata gcctagaatt
     1561 ttaaattgat gccaaaattg atactgcgtg cagccggata tgtgaagaaa tccgtcccga
     1621 tgaccgtatt gtcatcgccc aggtaattca cctcaggatc aagtcctgaa tagttggtga
     1681 atgtgaacag gttctg
//
LOCUS       DAOPXU010000221         1686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_110125_length_1686_cov_19.534641, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000221 DAOPXU010000000
VERSION     DAOPXU010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110125_length_1686_cov_19.534641"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="PKK03_12580"
     CDS             complement(<1..1214)
                     /locus_tag="PKK03_12580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TANFOR domain-containing protein"
                     /protein_id="HNX85275.1"
                     /translation="MPPYSNRLSDYIATPGKIYSVITPVATGFDRLEYPVFFHGVIMS
                     ADESVIIRTKSGYKPAAPIVIRGTQGPTGGIIFMPYTLTYSDILQIFPDQSLEYTGIT
                     REQVMQQGLPENTYRICFSIYDYDSSRPLTSVSACSNSFNVTWVEAPIIITPANKAQL
                     SETEAKNITFIWTRPPSAPVNTQYKLKIIELTPPDGNYQDKLQSAGYPVFFETTVFTN
                     TYLYSFANPQLIAGKTYAFMVTAIDPSNMTAFRNKGNSEPFIFSCRAGEQSQTPAGAD
                     SEFTFIIPRKLAQNKPDTLVVNENQDFLINWGWVKSVTPDSLALTDSEMIQSLGIEKY
                     VLDITRTKSNNPGTASFSFSKTFNKDSGGTITNKLQISKEAAQDAGFTDGDVYTASIR
                     QIGKTNNQIATYT"
     gene            complement(1306..>1686)
                     /locus_tag="PKK03_12585"
     CDS             complement(1306..>1686)
                     /locus_tag="PKK03_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85276.1"
                     /translation="DTDTKVAMLMYSLGIVKQKINVSLGANYTKMVNQNYTNQLAGGT
                     LGISSSLLKDKLTLNWNNAYMINKLNGDSGIIFNTSLNSNYRFLPRHSVTLNWNLIKN
                     SFADSSVVPSYNEIRGDLGYAFTF"
BASE COUNT          413 a          341 c          416 g          516 t
ORIGIN      
        1 gtgtaggttg ctatctggtt atttgtcttg ccaatttgtc tgattgaggc agtgtaaaca
       61 tccccgtcgg tgaatcctgc atcttgcgct gcctctttgc tgatctgaag tttattagtt
      121 atggttcctc cggagtcttt gttaaaggtc tttgagaaac tgaaggatgc tgtacccgga
      181 ttattgcttt ttgtcctggt aatgtcgagc acatattttt caatgcccag gctttgaatc
      241 atttccgaat cagtcagggc cagcgaatcc ggggtgacag acttgaccca tccccagtta
      301 ataagaaaat cctggttctc atttaccaca agagtatccg gtttgttttg agccagtttg
      361 cgcgggataa tgaaagtgaa ctctgaatct gctccggctg gtgtttgaga ttgctctccg
      421 gctctacagg aaaatatgaa cggttcactg ttgcccttat ttctgaaagc agtcatgttt
      481 gacgggtcaa tggccgtaac catgaaggca taggtcttcc cggcgataag ctgcggattg
      541 gcaaatgaat agaggtatgt atttgtgaaa acagttgtct caaagaacac aggataacct
      601 gctgactgta acttgtcctg atagttgcca tcaggaggcg tcagttcgat tattttcagt
      661 ttgtactggg tgttgaccgg tgcacttggg gggcgggtcc agatgaatgt tatgtttttt
      721 gcttcggttt cgcttagctg agccttgttt gcgggagtta taattattgg cgcttccacc
      781 caggtgacat tgaatgaatt actgcaggca gatacactgg taagcggtcg tgaggaatca
      841 taatcataaa ttgagaaaca gatcctgtaa gtgttttcag gcaacccctg ttgcataacc
      901 tgttcacggg taatgccggt atattccagg ctttggtccg gaaatatctg gagtatatcg
      961 ctgtaggtga gtgtgtatgg catgaaaata atgccgcctg tgggtccctg ggttcctctg
     1021 ataacgattg gtgcagccgg cttgtaaccg gattttgtac ggataatgac cgattcatcg
     1081 gcgctcataa tcaccccgtg aaagaatacc gggtattcca gcctgtcaaa gccggttgcc
     1141 accggggtga tgacgctgta aatctttccg ggcgtcgcta tgtaatcaga aagtctgttt
     1201 gaatatgggg gcatcacatt aacagtctgc acaaactgtg atctggccgt tacggaaaga
     1261 agaaaacaca acaggacgaa caatattcgt cctgaattcc taatcttaga aagtgaaggc
     1321 atatcctaga tctcctctta tttcgttata cgacggtacg actgaactgt cagcgaatga
     1381 attttttatc agattccaat tgagtgtaac cgaatgcctg ggaaggaaac ggtagtttga
     1441 attcagtgaa gtgttgaata tgattccgct gtctccgttc agtttgttaa tcatatatgc
     1501 attattccag ttcagggtga gcttatcctt aagcagggat gatgaaatac ccagagtacc
     1561 acctgccaac tggtttgtat agttctgatt gaccattttt gtatagtttg ctccaaggct
     1621 cacatttatt ttttgtttga caatgcccag ggagtacatc agcatggcga ccttcgtatc
     1681 tgtatc
//
LOCUS       DAOPXU010000222         1681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_110644_length_1681_cov_10.799508, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000222 DAOPXU010000000
VERSION     DAOPXU010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110644_length_1681_cov_10.799508"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1335)
                     /locus_tag="PKK03_12590"
     CDS             complement(133..1335)
                     /locus_tag="PKK03_12590"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNX85277.1"
                     /translation="MTRKELRIGLFGYGCVGRGLHDVLENSRGIKAEIVRICVKDRSK
                     ERRLPMSFFTFDKNDILNDSSINLVVELINDPEEAFAIVKQAMENGKSVVTANKVMVA
                     THLEELVRLQQENKVALLYEASAGASIPIIRNLEEYYDNELLHSLRGILNGTTNYILT
                     LMHNEGKDFAKALQEAQDKGFAEKDPGLDVDGWDAKYKLCIITAHAYGLFLDPDEVFH
                     YGIGNISGYDIQYAKEKGYKIKLVPYVGKTGEDTITGFVLPRFVSPDKYLYNVDHENN
                     GVITEAAFAEKQFFAGKGAGGHPTGSAVLSDISANSYGYRYEYKKNMQGTVRKFTLDH
                     KIEIYLRYHDERDRDLFRFSAVSEVYTNHRYSYVIGTVNLENLYRLRKDLGNMDVFLI
                     NTGRTVTG"
     gene            complement(1316..>1681)
                     /locus_tag="PKK03_12595"
     CDS             complement(1316..>1681)
                     /locus_tag="PKK03_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNX85278.1"
                     /translation="FRASSYQAYQGDKLVSRFNPYSYYCLTKLSDTHNAGRGRGGVRE
                     ALKAIRAKVLSVGISSDFLFPVYDQKFLADNVEGAYYKEIDSLYGHDGFLIERELLTG
                     IIREFWEKCSKDNYDKKRA"
BASE COUNT          391 a          448 c          369 g          473 t
ORIGIN      
        1 gtgaagggca attgcaggtc tatggcggaa tatatcatac gatttctgac ttcggtgtaa
       61 aggtatataa cgaagtgaaa tgagcccggc cctgattcaa aaataatgat gactccttca
      121 gtgtcttatc tgtcagcccg ttacagtcct gcctgtattg attaaaaaca catccatatt
      181 cccaagatcc ttcctcaggc gatataggtt ttcgaggttc accgtaccga ttacatagct
      241 gtacctgtga tttgtgtaaa cctcgcttac cgcactgaac ctgaagagat cccggtctct
      301 ttcatcatga tacctcaggt atatttcgat cttgtggtca agggtgaatt tccgtacggt
      361 gccctgcatg ttcttcttgt actcatacct gtaaccgtat gaattggcag aaatatccga
      421 gagcactgcg cttcctgtgg ggtgaccgcc agcacccttc ccggcgaaaa actgtttctc
      481 ggcaaaggca gcctcggtta tcacgccgtt gttttcatgg tccacattgt acaggtattt
      541 atccggtgag acgaaccggg gcagtacgaa acctgtgatt gtgtcttccc cggtttttcc
      601 cacatacggg accagcttga tcttgtagcc cttctccttt gcatactgta tgtcataccc
      661 ggagatattc cctatgccgt aatgaaaaac ttcatccgga tcaaggaaca gtccgtaggc
      721 gtgcgcagtt atgatgcaga gtttatactt cgcatcccat ccgtcaacat ccagtcccgg
      781 atccttctcg gcaaaaccct tgtcctgagc ctcctgcagc gccttcgcga agtccttccc
      841 ctcattgtgc atcagtgtca ggatatagtt ggttgtaccg ttgaggatgc cgcgaagcga
      901 atgaagcagt tcattgtcat agtattcctc aaggttcctg ataattggaa tgctggcacc
      961 ggcagatgcc tcatacagca atgcaacctt gttttcctgc tgcaatcgca caagttcttc
     1021 aaggtgggtg gcaaccatta ccttgtttgc ggttacaaca ctcttcccgt tctccatggc
     1081 ctgttttaca atggcgaatg cctcctctgg atcatttatc agctcaacaa ccaggttgat
     1141 tgaactgtca ttcaggatat catttttgtc aaatgtaaag aaggacatgg ggagccgtct
     1201 ctccttcgat ctgtctttca cgcaaatgcg gactatctcc gcctttatcc ccctgctgtt
     1261 ctccagcacg tcgtgcagtc cccggccgac gcatccatat ccgaacagcc ctatcctaag
     1321 ctcttttctt gtcataatta tcttttgaac atttttccca gaattccctg atgatgccgg
     1381 tgagcagctc cctttcaata aggaaaccgt catggccgta aagggagtca atctctttgt
     1441 agtaagcacc ttcgacattg tctgcaagaa acttctgatc atacaccggg aaaaggaagt
     1501 ctgacgagat cccgacagag agaaccttag ccctgatggc tttcagcgcc tcacgcaccc
     1561 cgcctcttcc gcgtccggca ttatgcgtgt ctgacagctt cgtcaggcaa taatatgaat
     1621 acgggttaaa ccggctgaca agcttgtcgc cctggtatgc ctggtatgac gaagccctga
     1681 a
//
LOCUS       DAOPXU010000223         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_114726_length_1643_cov_7.907431, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000223 DAOPXU010000000
VERSION     DAOPXU010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114726_length_1643_cov_7.907431"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..116
                     /locus_tag="PKK03_12600"
     tRNA            43..116
                     /locus_tag="PKK03_12600"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:77..79,aa:Ala,seq:tgc)
     gene            complement(779..1336)
                     /locus_tag="PKK03_12605"
     CDS             complement(779..1336)
                     /locus_tag="PKK03_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85279.1"
                     /translation="MKKVLFVIVAGIIASAHLNAQESSFLKGDKVLNLGLGLGSTLYS
                     GSYYVGQIPPVSASLEIGIVDGLAEKGVIGIGPYLGYSSYKYEYSGYGWKYSNIILGA
                     RGSFHYPLVDKLDTYAGVLLGYNIVSSKEFGDPIPGNDDSFASSGLAWSGFIGARYFF
                     SDAIAAMAELGYGIAWLNLGVALKF"
BASE COUNT          461 a          367 c          385 g          430 t
ORIGIN      
        1 tcgggagggt caagggttct gaatccggtt ctttacaaaa ttggggattt agctcagttg
       61 gccagagcac ctgccttgca cgcagggggt caagggttcg actcccttaa tctccacgga
      121 agagtcttca gaaatgaggg ctcttttttt tcagcctttt acgaagactt gattcagcaa
      181 tgtggctcag aaacaattgc ttctcaacag caaaaaatat tagtaatacc aagacagaac
      241 aatcccaggc gggattcact cctccccgga cttaatcaca tgataggagg tagattctat
      301 gtcattggaa aagtttatct gcagctttcc gggatcagtt gtaaccaggt ctgttacagt
      361 cactgtccat tttatgtctt tattgtcttt gcactggtag gctatggcaa tatcgtcgtc
      421 ttcatcagtt tcacttttcc cgacgctgat aatcttatac tcggtaacac tcagccacac
      481 gggatcaaag agggtaatta cgcccccctt gccatctatt ttgatctcaa cgttttcttc
      541 aatccagtca gtccactcgc cgtcatcatc gatgtagcag gaatagcctg agatgtaggt
      601 ctgtgcctta agggcaggga tgctggtgat aaacagcagg gccaggatat ttgtaatctt
      661 tttattgccg gtaaaaatta gccgggcata aaattagtga aaggaatagt tccggagaac
      721 tcattccctc actgtaaaaa gtgttttaca gccactccaa ttcctgacag gaagagactc
      781 aaaacttcaa agcaacaccc aggttgagcc aggcgatgcc ataaccaagc tcggccatcg
      841 cagcaatggc atccgagaag aaataccttg cccctatgaa gcctgaccag gcaagcccgc
      901 tggatgcaaa tgagtcatca tttccgggga tcggatcacc aaactctttg gaggagacaa
      961 tgttgtatcc cagcagtact cctgcataag tgtccagttt gtcgaccagc ggataatgaa
     1021 agcttcctct tgctcccagg attatgtttg aatatttcca gccatatcct gaatactcgt
     1081 atttgtagga tgaatagccg agatatggac ctataccgat cacccctttt tctgccagtc
     1141 cgtcgacaat accaatttca agggaagcag atactggcgg aatctgccct acatagtagc
     1201 tgcccgagta aagggttgat ccgaggccca gacccaggtt cagcaccttg tcacctttaa
     1261 gaaacgatga ttcctgtgca ttgagatgag cagaggcaat aatgcctgcc acgataacga
     1321 aaaggacttt tttcatgatg gttgtttttg gggttaaata caagcaatat aacaaccggt
     1381 gaagtctctt gttcaggagg gtgctgtctg ctgacgggct gggaggggtg gtgcgacagc
     1441 ctcaggatta gcgactgaga atgaataaca gatcgtgtaa tagcttaaat gacaggatgg
     1501 tatcggaagc ttgagtggta acaacgggga ttgcactgaa ataaaatctc tcaggagtga
     1561 tgtaaaaaat gcaacaaaaa tcgcggaatg cagttataat aggtacctgt aaaacaaaaa
     1621 ggcggtcccc attcatatgt tag
//
LOCUS       DAOPXU010000224         1638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_115301_length_1638_cov_13.975363, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000224 DAOPXU010000000
VERSION     DAOPXU010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115301_length_1638_cov_13.975363"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PKK03_12610"
     CDS             <1..391
                     /locus_tag="PKK03_12610"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HNX85280.1"
                     /translation="AIGLFAIFTILNLRTISFNVKNMAYLFAVIAISVLNALDLRGKF
                     PFMGVVIFNLMIIASAFILERLLARKKKYSTHPIIYDNLEMLKSQNNEALIADIAEIT
                     GLDVRKVKIQSVNYKERTAVLDIYYRT"
     gene            complement(392..1354)
                     /locus_tag="PKK03_12615"
     CDS             complement(392..1354)
                     /locus_tag="PKK03_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85281.1"
                     /translation="MFEEEDLLKASLRFDVVKFLKKAERDQSVDGILTLHFSETDELE
                     RKVTLKYRGQSRFERCKYPPTRITFKKPLYEVSDSGRIKNLKLVNQCQQGSSYGDYVI
                     REYLVYKLYNVLTDTSFRARLISLDIIDSENKRKTITQYGILLEPESLLEERLDLVEV
                     KTKNLSQSHMFPAMIDRLAIFNYMVSNWDWSVPGQHNVSVFTSVNPTLGGVGIPVPFD
                     FDLTGVVNAEYAIPPPDLGIETNRDRIFYGICRTEEVYRRELLAFREKKEELYSVITE
                     FPHLGRAEKRDIITFMDQFFDRLEKPRSVEGLIDYLLENCKGSR"
BASE COUNT          412 a          371 c          351 g          504 t
ORIGIN      
        1 ggcaatagga ttgttcgcga tatttaccat actcaatctc aggactataa gttttaatgt
       61 aaagaacatg gcatacctct tcgcggttat tgccatttct gtccttaacg cgcttgatct
      121 ccggggtaaa ttcccattta tgggtgttgt gattttcaat cttatgatta ttgcatcggc
      181 atttattctt gagcgcctgc ttgccaggaa gaaaaagtac agtactcacc cgatcattta
      241 tgataacctg gagatgctga agtcccagaa caatgaggca ttgatcgccg atattgccga
      301 aattaccggg ctcgatgtca ggaaggttaa aatccagagt gttaactata aggagaggac
      361 tgcagttctg gacatttatt acaggacctg atcaacggga acccttgcaa ttttcaagca
      421 ggtagtcgat cagaccttca acgcttcttg gtttttcaag ccggtcaaag aactgatcca
      481 tgaaagtgat aatatctctt ttttcagctc tgcccaggtg agggaattca gtgattaccg
      541 agtaaagttc ctccttcttt tccctgaacg ccagcagttc tctccgatat acctcctcgg
      601 tgcggcagat accgtaaaat atacggtcac gattggtttc tatccccaga tcaggcggag
      661 gaatggcata ttcagcattt accactccgg tcaggtcaaa gtcaaacgga accggtatgc
      721 ctactcctcc aagcgtggga ttcacggatg taaagaccga aacattatgc tgtcccggca
      781 cggaccagtc ccagtttgac accatataat tgaagatggc gagcctgtca atcattgccg
      841 gaaacatatg gctctgcgac aggttttttg tttttacctc aaccaggtct agtctttctt
      901 cgagaagact ttccggttcg agtagaattc catactgggt gattgttttc cttttgttct
      961 ctgaatcaat aatatccagg cttattaatc ttgccctgaa gcttgtatca gtaagaacat
     1021 tgtacagctt gtaaacgagg tattccctta tgacataatc accatatgat gatccttgct
     1081 ggcattggtt gaccagcttc aggttcttta tcctgccgga atctgatact tcgtacagag
     1141 gtttcttgaa agtgattcgt gtcgggggat atttgcatct ttcgaaccgc gactgaccgc
     1201 ggtatttaag tgttactttt ctttccagct cgtcagtctc actgaagtgc agggtcagaa
     1261 tcccatcaac agactgatct ctttctgcct ttttcaagaa cttcacgaca tcaaaacgca
     1321 gggaggcttt gagaagatct tcctcttcaa acaacgataa gtatctttcc ggaggtttct
     1381 gatcctgctg cactttattg tttgcatctt tccggctttt cggctcctgc cttgaagtat
     1441 cagccggatt taccgtcttt tttgcagttg tatcaatctg accgttagtc ggcaggactg
     1501 aaaacacgaa tataagaagc gagcttaaac acaggcgatg ctcagcgctg cacttgtcaa
     1561 gataggtccc ggctttcatt ttattctgta tctgaataac attatggaag caggatttgt
     1621 tctgccttca tttgatat
//
LOCUS       DAOPXU010000225         1609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_118649_length_1609_cov_9.823037, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000225 DAOPXU010000000
VERSION     DAOPXU010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118649_length_1609_cov_9.823037"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..631
                     /locus_tag="PKK03_12620"
     CDS             107..631
                     /locus_tag="PKK03_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNX85282.1"
                     /translation="MGKVDRDFYLADAVTVARVLPGMELIMTSGEKCLRYVITETEAY
                     LGDGDRACHASRGRTRRTEPMYLEGGHLYIYFVYGMHWMLNVVTGPADHPQAVLIRGV
                     SQYQGPGRVTKGLGVDGSFNREDLASSGRIWIEDTGFRPALIAGPRVGIDYAGEPWIS
                     KPWRFIIKNEKEKI"
     gene            628..>1609
                     /locus_tag="PKK03_12625"
     CDS             628..>1609
                     /locus_tag="PKK03_12625"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNX85283.1"
                     /translation="MKNGFRLWNNITGWAVFAVSLVIYLLTIEPTVSFWDCGEFILCS
                     YRLEVGHPPGAPFFMLLGRFFTLFAGGDTSKVAMMVNILSAFASAFTILFLFWTITRL
                     VRLATGDDETVTGGKSLATLAAGAIGALAYAFSDTFWFSAVEGEVYGTSSLFTAAVVW
                     AMLRWYDEADDPRSGRWIILIAYLMGLSIGVHLLNLLALPVIVLIWYFRKHKPSTRGF
                     VYALLAGFLILGVLNFVFVPGVPKVAGWFELFFVNTLGLPYNTGLYIYLIILTGLIAG
                     GIWYSLKKGRRILNFVITVIAVLVLGYSSFALIIIRANAHPPMNQNDPSDV"
BASE COUNT          355 a          426 c          458 g          370 t
ORIGIN      
        1 tggcagcaga aacatcgaga tggtccgcgg actgaagaag gagggagaca gcatactgga
       61 aaggtgggcg gaactccctg acaagataca gtactgaaca gcagcgatgg gcaaagtcga
      121 cagggatttc tatcttgcag atgcagttac agtcgcccgc gtgctgccgg gcatggaact
      181 gatcatgaca tccggggaga agtgcctcag atatgtaatt acggagacgg aggcctacct
      241 gggtgatggt gacagggcat gtcatgccag caggggaaga accaggagaa cggaaccgat
      301 gtacctcgag ggagggcatc tctacatata cttcgtctac gggatgcact ggatgctgaa
      361 tgtggttacc ggtcccgctg atcatcccca ggctgtgctc atcagagggg tttcacaata
      421 ccagggacca ggacgcgtca caaagggact gggagttgac ggttcattca acagggagga
      481 cctggcatcg tcaggcagga tctggataga ggacaccggc ttccgcccgg cgctgatcgc
      541 agggccgagg gtgggaatag actatgccgg tgagccatgg ataagcaaac catggcgctt
      601 cattataaag aacgaaaaag aaaagatatg aaaaacggat tcagactgtg gaacaacata
      661 acgggctggg cagtgtttgc cgtctcgctt gtcatctacc tgctgaccat cgaacccact
      721 gtcagcttct gggactgcgg ggagtttatc ctctgttcct acaggctcga ggtgggccat
      781 ccgcccggag ccccgttctt catgctcctg gggcgtttct tcaccctctt cgccggaggc
      841 gatacctcga aggtggccat gatggtaaac atactttccg cttttgcgag tgcctttaca
      901 atcctgttcc tgttctggac catcaccagg cttgtccgcc ttgccaccgg tgatgacgaa
      961 accgtaaccg gaggaaaatc cctggcaact ctggctgccg gagccattgg cgcactggca
     1021 tacgcttttt cggatacctt ctggttctcg gcggttgagg gtgaagtcta tggcaccagc
     1081 tccctgttca ctgctgcagt ggtatgggca atgctccgct ggtacgatga agccgacgat
     1141 ccccgctcag ggagatggat cattctgata gcctatctca tgggcctttc aatcggggtt
     1201 cacctgctga acctgcttgc cctgccggtg atagtcctga tctggtactt caggaagcac
     1261 aagccatcga cccgtggctt cgtctatgca cttcttgccg gtttccttat cctgggcgta
     1321 ctcaacttcg tgttcgtgcc gggtgtgcca aaggttgccg gatggtttga actcttcttc
     1381 gtaaacaccc tgggcctgcc atacaatact ggcctttata tctatctgat catccttacc
     1441 ggcctgatag ctggaggcat ctggtattcg ctgaagaaag ggcgcaggat cctgaacttc
     1501 gtcataacgg tgattgccgt cctggtgctg ggttactcat cgttcgccct gatcatcata
     1561 agggccaacg cccacccgcc catgaaccag aatgacccgt cagatgtct
//
LOCUS       DAOPXU010000226         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_121455_length_1585_cov_14.674510, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000226 DAOPXU010000000
VERSION     DAOPXU010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121455_length_1585_cov_14.674510"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PKK03_12630"
     CDS             complement(<1..335)
                     /locus_tag="PKK03_12630"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295947.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HNX85284.1"
                     /translation="MRTKEDIVNNWLPRYTGKELGSFGEFILLTNFTHYVETFARWND
                     VQVEGKDKNWPNATAGGVTIINFGMGSPNAATVMDLLSAIQPKAVLFLGKCGGLKKKN
                     KLGDLILPIA"
     gene            398..862
                     /locus_tag="PKK03_12635"
     CDS             398..862
                     /locus_tag="PKK03_12635"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HNX85285.1"
                     /translation="MKPLKLPPCSLRIKEGPGNRLVYDPLRKKYVALTPEEWVRQHFV
                     NYLTGFLGYPPGLIKVETSFRLHGMLRRADILVHDRDGEPVLIVECKAPQVKISQEVF
                     NQVINYNFSYCVRYLVVTNGITHFAGKIDTAGRSFNLLDHIPDYETITKQQP"
     gene            859..>1585
                     /locus_tag="PKK03_12640"
     CDS             859..>1585
                     /locus_tag="PKK03_12640"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HNX85286.1"
                     /translation="MIDVTCAVIRNEEGLVLAVRRGPGMGNAGKWEFPGGKTRAGEDH
                     EECILREIDEELGMDIVICGKLDYVEHDYGNKQIRLIPFICDTLASKPALREHDQYRW
                     MQPEKLTGLDMTAADIPVAAQYVAGHRCEVNRETEEDTARITAEAGEELAKMIARVSS
                     VEEITMVAQSAVSDTNLLSQLVTLCSSTEKRVGFMASWALSKVVDVDPKMMAPFLPLI
                     IDALPSAPNESVQRSFMRVLTKSE"
BASE COUNT          377 a          392 c          455 g          361 t
ORIGIN      
        1 gctataggaa gaataaggtc acccagcttg ttcttcttct tcagccctcc gcacttgcca
       61 aggaagagca cagccttcgg ctggatggca ctgaggaggt ccatcaccgt ggcagcgttg
      121 gggctcccca tgccgaagtt aatgatggtg actccaccgg cagttgcatt gggccagttc
      181 ttgtcctttc cctccacctg cacatcgttc cacctggcaa aggtttccac ataatgtgta
      241 aaattcgtca gcagaatgaa ctcgccgaat gagccgagtt cttttcccgt gtagcggggc
      301 agccagttgt ttactatatc ttccttggtc ctcatgttct cagatgctgt ttgtttatat
      361 ttgccctgcc taagggttac caaaaataca ataaaagttg aaaccgctga aactgcctcc
      421 ctgttcactg cggattaaag agggtccggg caacaggctt gtctatgacc cgcttcgaaa
      481 aaagtacgtg gcactcactc ccgaggagtg ggtcagacag cacttcgtca actacctgac
      541 cggtttcctg ggctatccgc ccggacttat aaaggttgag acctcattca gactccacgg
      601 gatgttgcgg cgggctgaca tcctggtgca cgacagggac ggggagccgg tgctgattgt
      661 cgagtgcaag gcgccccagg tgaaaatctc ccaggaggtc tttaaccagg taataaacta
      721 caacttcagc tactgtgtca ggtacctggt ggttaccaac ggcataacac acttcgcggg
      781 gaagattgat accgcaggac gaagctttaa tcttctcgat catattcctg attatgaaac
      841 cattacgaag cagcagccat gattgacgtt acctgtgccg tcatacggaa cgaggaggga
      901 ctggtgctgg cggtgcggcg cggtcccggg atgggcaatg cgggaaaatg ggaatttccg
      961 gggggaaaga cccgggcagg agaggatcat gaagagtgta tcctcagaga gattgacgag
     1021 gagctgggga tggacatcgt tatctgcgga aaactggatt atgtggagca tgattatggc
     1081 aacaaacaga tcaggctgat tcccttcatt tgcgataccc tggcgtcaaa gccggctctc
     1141 cgggagcatg atcagtacag gtggatgcag ccggagaagc tcaccggcct tgacatgacg
     1201 gcagcggata tacctgttgc cgctcagtat gtggccggtc acaggtgtga ggtaaaccgt
     1261 gagacagaag aggatacggc caggattact gccgaggccg gcgaggagct tgcgaaaatg
     1321 atcgccaggg tctcaagtgt tgaggagata accatggtgg cccagtcggc ggtatcagac
     1381 acaaacctgc tgtcacaact ggttaccctc tgttcaagca ctgaaaagag ggttgggttt
     1441 atggcctcgt gggctctctc gaaggtggtt gacgttgacc cgaagatgat ggcgccattc
     1501 ctcccgctga ttattgacgc acttccctcg gcacccaatg agagcgtgca gaggtcgttc
     1561 atgagggtcc tgacgaagag tgaaa
//
LOCUS       DAOPXU010000227         1542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1332
            NODE_126801_length_1542_cov_11.146604, whole genome shotgun
            sequence.
ACCESSION   DAOPXU010000227 DAOPXU010000000
VERSION     DAOPXU010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854537
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:26:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,502
            Genes (coding)                    :: 2,492
            CDSs (with protein)               :: 2,492
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126801_length_1542_cov_11.146604"
                     /isolate="AMR_MDS_1332"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="PKK03_12645"
     CDS             complement(<1..1093)
                     /locus_tag="PKK03_12645"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX85287.1"
                     /translation="MNRARTILIAVITCISVNISAQQVYDLQSCLKFALENNHNIRKA
                     QFDKEKSAEARHEILGALLPQISGTGNLNYNIQKSRFIMPNFINEFLPPNMQDPNAAK
                     YMTIEMGTNFSAGIGAALNQQIFNLSLFNAVDIARTAEELTSLAADAKEEDVISQTAS
                     LFYAVQATEYAAGMFGRSIEIIDSVLTSMETSYASGLIRKVDLDRLKVTRTNMATQRA
                     SVLNAVEVQKNLLKLQMGMDMSGRIDIEKINLEAFENQAKPGNQQNFDPNLQTSYKML
                     ARQHNLRLLQKKAAIYENYPVLTAMINYNYNGVSDEFFRGETNYWYGSSMAGLNLRIP
                     IFGGLSRSSKIKQADFEIRKIKEDGVILEQ"
     gene            complement(1093..>1542)
                     /locus_tag="PKK03_12650"
     CDS             complement(1093..>1542)
                     /locus_tag="PKK03_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX85288.1"
                     /translation="VHDLEKKISIMYSEDVNAIEGFKSGICGMGEVFHSLSPVFFDDL
                     RKMFPELSGTQHKAGFGSFLEGMKRNLEKGKKEKLYRQEINVEMISRFFISSVVSFFI
                     SSVLTRSEYSARDYFRSVIDYHLHAVVTPKGKRLLDKENLTTKYSEK"
BASE COUNT          361 a          377 c          333 g          471 t
ORIGIN      
        1 actgttcaag aatgactccg tcttccttga ttttgcggat ttcaaagtca gcctgtttta
       61 tcttcgagct tctcgaaagc cctccgaaaa tagggatgcg caggttcagg cctgccattg
      121 acgatccgta ccagtaattg gtttcacccc tgaaaaactc atcggatacc ccgttgtaat
      181 tatagtttat catcgccgtt aaaacaggat aattttcata aatggctgcc tttttttgaa
      241 gcagccggag gttatgctga cgggccagca ttttgtacga agtttgaagg ttgggatcga
      301 aattctgctg gttcccgggt tttgcctggt tctcaaatgc ttcaagattg atcttctcga
      361 tgtcaatccg gcctgacata tccatcccca tctgcagctt cagcaggttt ttctgcactt
      421 ccacggcatt gagaaccgat gccctctgtg tggccatgtt cgtcctcgtg acctttaacc
      481 tgtcaaggtc aactttcctt atcagcccgc tggcgtaact tgtttccatc gaagtcagga
      541 cagagtcaat gatttcgatg ctccggccaa acattccggc agcgtattcc gtcgcctgaa
      601 cagcatagaa caggctggca gtctggctga taacatcttc ctccttagcg tcggcagcca
      661 gtgatgtcag ctcttcggcc gttctggcga tatccaccgc attgaacagt gagaggttaa
      721 atatctgctg gttcagggca gcccctattc cggctgaaaa gttggttccc atttcaatgg
      781 tcatatattt ggccgcattg ggatcctgca tgttcggagg aaggaattca tttatgaaat
      841 tcggcattat aaacctcgat ttctgaatgt tgtaattcag gttgccggtg cctgatatct
      901 gcggcaacaa tgcaccaagg atctcatgcc tggcctcagc cgatttctct ttgtcgaact
      961 gagccttgcg gatattatga ttgttctcca gtgcaaattt caggcagctc tgtagatcgt
     1021 aaacctgctg tgcgcttata ttcacgctga tgcaggtgat tactgcaatt aaaatagttc
     1081 tggctctatt cattatttct cagaatattt tgtggttaaa ttttccttat caagcagtct
     1141 tttccccttc ggggtgacaa ccgcatgaag gtgatagtct attacggatc tgaaatagtc
     1201 tctcgccgaa tattcactgc gggtcaggac actgctgata aagaagctta caacagagct
     1261 tatgaagaat ctgctgatca tttcaacatt aatttcctgc ctgtacagct tttccttttt
     1321 ccccttttcc aggtttctct tcatgccttc gagaaaagac ccaaatccgg ctttgtgctg
     1381 tgtgcctgag agttcgggga acatctttct caggtcatca aaaaagaccg gactcagtga
     1441 atgaaagacc tcccccattc cgcagatgcc tgacttgaac ccctcaattg cattgacatc
     1501 ttcagaatac attatggaaa tctttttctc cagatcatgg ac
//
